LOCUS       DAOTGZ010000001        42922 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_3064
            NODE_923_length_42922_cov_6.823151, whole genome shotgun sequence.
ACCESSION   DAOTGZ010000001 DAOTGZ010000000
VERSION     DAOTGZ010000001.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868088
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 42922)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 42922)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:22:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,785
            CDSs (total)                      :: 2,751
            Genes (coding)                    :: 2,732
            CDSs (with protein)               :: 2,732
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 12 of 19
            Pseudo Genes (internal stop)      :: 5 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..42922
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_923_length_42922_cov_6.823151"
                     /isolate="AMR_MDS_3064"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..2090)
                     /locus_tag="P5123_00005"
     CDS             complement(<1..2090)
                     /locus_tag="P5123_00005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007935332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004356 - glutamate-ammonia ligase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine synthetase III"
                     /protein_id="HRX14674.1"
                     /translation="MSNFSNIFGVKVFNDAVMREMLPKATYKALKKTIDNNEKLAPDV
                     AEVVASVMKDWALEQGATHFSHWFQPMTGITAEKHDSFISPTGDGRVILEFSGKELIM
                     GEPDASSFPNGGIRATFEARGYTIWDCTSPAFVKDNTLCIPTAFASYTGEALDKKTPL
                     LRAMDAINKQGMRVLRALGDNESKKLITYVGAEQEYFIIDKDMFDARKDLKYCGRTLF
                     GAAPSKGQDMDDQYFTAIKVRVMDFMIDLDQELWKLGIPSKTRHNEVAPAQHEIAPIF
                     CTTNVGTDQNQLLMETIKKVAARHDLACLLHEKPFAGVNGSGKHNNWSMGTDTGRLLT
                     NPGNAPHKNDEFLLMLSVVMRAVDKYADAIRSSAAVPGNDHRLGANEAPPAIISIFVG
                     AQLEDIINQIVQKGCASSCISAEQMRLGASTLPDMDKDTTDRNRTSPFAFTGNKFEFR
                     MPGSSQSIAGPNFVINAAIAEVFSEVADRLESASDVKAEVQKIIVEYMKEHRRIIFNG
                     NGYSEDWVKEAEKRGLPHHRNLVDAKLALIAPKNVKMFSDMGVLSERECHARYEILLE
                     HYVNTIKIEAMTALELAKRDLLPSTFRFITDLAHSINAVKATGIAATLEAQESLIIEV
                     SDLASSAKKKIAVLDKAIEQTLAIDDVPKQAAAFRDNVVTAMNDLRLDFDRLELLVDS
                     EYWPLPTYGEMLFSV"
     gene            complement(2618..2986)
                     /locus_tag="P5123_00010"
     CDS             complement(2618..2986)
                     /locus_tag="P5123_00010"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRX14675.1"
                     /translation="MKVALKKLGVILFLLFFVLSFAGCEDDEDKAQGRISNMTDYDFL
                     NVKFADQNMGDVIANTISEYKDLKLEGGFLQFDVAIEGVYETYIYEEYLEAPKKDKKY
                     TFVITYSEYTEKFSGYPVED"
     gene            3494..5641
                     /gene="recQ"
                     /locus_tag="P5123_00015"
     CDS             3494..5641
                     /gene="recQ"
                     /locus_tag="P5123_00015"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: protein motif:HMM:TIGR01389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA helicase RecQ"
                     /protein_id="HRX14676.1"
                     /translation="MHKANQLLKEIFGYSSFRPVQQQAIKSVLDKHDTIAIMPTGGGK
                     SLCYQIPALIFNGLTLVVSPLISLMKDQLDQLLELGIEAGMLNSTLSDDEYAQTVSRI
                     KEGKIKILYCAPETLLQQRTINLLDSTKLSLIAVDEAHCISEWGHDFRPEYRRIAEVR
                     SRFNDAVCIALTATATDQVRLDIASQLKIKPDSVMVASFDRPNLFIDVIPKTDPQKQA
                     IDFIQQFSGQPGIIYCFSRRQVDELSEYLNNNGINNRPYHAGLTDNERQTNQNLFRND
                     DINIITATVAFGMGINKPDIRFVLHYDMPQNIESYYQQIGRAGRDGLPAHCRLLFGYG
                     DINKIQFFINQKNDSEKKTAYNKLNDLLSFLEKRECRRVPLMTYFSEKPSSQKCDNCD
                     NCLTPPPDEKEYTIQAQKFLSCIIRLDQRYGASLIIDVLRGSKSEKVKKSGALNISTY
                     GIGSELSKSEWMNLFRQMSSKGLLKVNTETGSLIVTKVGADILKGKQKFYGYITPAAS
                     PKASVKKSYLQNSELFEQLRTLRKQIADADGVPPYTVFPDKTLIEMTQFLPQDPDDLL
                     MIYGVGHNKLQKFGNQFLDIIKKFCINNPSNNHQRITANNKNRKESAHSISSKMYQAG
                     TPVHEIAQRLSIQTSTVIDHLYKWISEDNPLDCEALLDEFNISEELCFRVHSLFEKHG
                     VFKLKPVFEEAAGEIDYEILKILRLWYITGKNS"
     gene            5873..6718
                     /gene="dapF"
                     /locus_tag="P5123_00020"
     CDS             5873..6718
                     /gene="dapF"
                     /locus_tag="P5123_00020"
                     /EC_number="5.1.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011971823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008837 - diaminopimelate epimerase
                     activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopimelate epimerase"
                     /protein_id="HRX14677.1"
                     /translation="MKSLKFVKIHGIGNDYIYIDGINSTINKLSDLSIKISDRHFGVG
                     SDGLIALLPSQKADFRMRMFNADGSEAEMCGNGIRGLAKMIHDHGFSKKKQLTIETLA
                     GIMELELTLKDNKVSMVTVDMGEPILEREKIPMIGEPGHVIEETIELEDSVKFELTAV
                     SVGNPHAIIFVEDVDNFPIEKYGPLIEKHPLFPSRINVEFVKILSSNEAKQRTWERGS
                     GETMACGTGATAVTVAGVLTGRLDSQSTIHLLGGDLMIRWDKQTNHLFMTGPATEVFK
                     GEWYY"
     gene            complement(6744..7598)
                     /locus_tag="P5123_00025"
     CDS             complement(6744..7598)
                     /locus_tag="P5123_00025"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRX14678.1"
                     /translation="MSKTFIKLSGFVNYFALVVFLLFIPSITAIGADSRQLSLIRDFT
                     DFYPLWFKSLDLASKYEKFESNKVLTLYNSEKNIIARGPANKKEEGYLFYGRWIRFYP
                     GGGRLAVINYKNGYLEGNASGYYKNGSRAITAQYKAGYLHGKMTRYDSRGRLLDVIEY
                     DNGQAVKHEIKIMLLPSFQRPGNIPPSAVYNEKDDIWIQFSEKSGFTKIWYRNGEPFC
                     EIASFKNDTKIIYHGIAVYWDKKGNRILEGFFKQGKRHGTWQNYNESGAVINKTIYQN
                     GEASDSEE"
     gene            complement(7591..8172)
                     /locus_tag="P5123_00030"
     CDS             complement(7591..8172)
                     /locus_tag="P5123_00030"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRX14679.1"
                     /translation="MKYYKVIMNKIKDYRILIRRDLRERNRFFLLMVSLMLILNYFLY
                     CFHVEKNIFDIYPSFPVMEKREDITIYLPGVKGEILEETRLVELENSKEKTIEFIVAE
                     IVRGSKYENTRMVVPIEFNIRKVWIHGSECYIDIRLETLNPETPILPESEGNFKKAVE
                     KSITANYNEIKSVAFLENGISNRNLWEKTIINE"
     gene            8325..9977
                     /locus_tag="P5123_00035"
     CDS             8325..9977
                     /locus_tag="P5123_00035"
                     /inference="COORDINATES: protein motif:HMM:NF014877.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRX14680.1"
                     /translation="MKKDILPKPYQIPKMGTSGLRLKQEQYNRAGFLEQFTEGIARYF
                     KEITSTTKIAENNTHLLLGGDPREGNSERIKTMACILTAHGFTVIVPENGLASTPAMS
                     HAIRHLKTIGAIILTASHNPFTDVGIKINTANGAPALTDCTERINEFQNDVQDYLTQD
                     YETALTEGLIKTIDIISMYADLLDKMFSFKDMREKIEDNPVSAVFDSMNGAAGPFARE
                     VFVNRLGLDPLMLREEPRTDLGGFDDKGEPKHPEPDFDYVSDLINHNSTNQFSIAAAW
                     DSDVDRRLDGGSGFFLESADEFAIFARYSHLINIQSMFNDTIYFCRSTVTSHAIDLMS
                     NELTAIFKDKTIKTVQTPTGFKWIAELGNWGVEESNGVGNPALREKDGIFATVFLLKI
                     ILETGKTPLQLMEDIWQDHGRVYFTRGEVSGSDPIEKEALINILNGAQSQIGNKFGTL
                     TLENAGSWDYKHPETGEIAEKNAAYYFEFSDGNSIKARFSGTGSGGYTLRVYCSKFDK
                     NYSIPKTTIVQPMKDAFDAFLQNGGFKGKSDKYTDSNQPDIY"
     gene            10210..10674
                     /locus_tag="P5123_00040"
     CDS             10210..10674
                     /locus_tag="P5123_00040"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRX14681.1"
                     /translation="MEKNDIRTSIDVGTKWLEKIEEYSKAKKISISDVINSVLDKYMK
                     ELADKPETYNRAMKYQARGTDYQSVHIRLSFISYSRLRDVMLVFRYSLSYMLSLAMEF
                     FDEYMNESEKEFFPPSFHCSIISSTSVPKFFSNYWHFPPEEHLLYEKPFEAG"
     gene            complement(10708..11685)
                     /locus_tag="P5123_00045"
     CDS             complement(10708..11685)
                     /locus_tag="P5123_00045"
                     /inference="COORDINATES: protein motif:HMM:NF013700.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan DD-metalloendopeptidase family
                     protein"
                     /protein_id="HRX14682.1"
                     /translation="MLYSVKRKIVEKRHVLRRKIAKKWSSFLEHGHEKMTIMFIPHDE
                     KKIFNFQISKFTISFFVVLFMTVVTTSSFAYMKNNQIKSEEEQLIENYREIHNQLKSY
                     EKMTKEVSQLMSEIRPEIEDLYEMAAGSEDISEIWSMVQNYDAVSEEELAHLKKVLPS
                     EVFELKKLQSDIACATNTVKTVRNFVDVRSQVINDTPSIIPNRGHITSLFGWRRSPFG
                     TGRDFHTGIDIAAPEGTPIRATAPGKVIMVGWGGGYGKMVRVQHKYGFKTFYAHQSSI
                     IVKTDDYVKKGQVIGYVGHTGASTGNHLHYEVRLGNVPINPYPYMSRMW"
     gene            complement(11802..12323)
                     /locus_tag="P5123_00050"
     CDS             complement(11802..12323)
                     /locus_tag="P5123_00050"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRX14683.1"
                     /translation="MGSLRMLVLSMIMVSVFFVWTENKSDRLASGEKEIELVKDELVY
                     KIRYESRGSRSERKIGLLSYRSKKIPYLFNYIQYKDSAYKLANAKRRWGNVGYQPTIK
                     KEIEKSSEKAVNDGAPFALTKVKFADIPDDWAYVEWENGSAFIDLNRFNEIITMLPFK
                     DLPVLFGIKALKR"
     gene            12432..14045
                     /locus_tag="P5123_00055"
     CDS             12432..14045
                     /locus_tag="P5123_00055"
                     /inference="COORDINATES: protein motif:HMM:NF012356.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-amylase family glycosyl hydrolase"
                     /protein_id="HRX14684.1"
                     /translation="MKEKHIWWKHGVIYQIYPRSFYDSNNDGIGDIKGITLKLDYLKE
                     LGISGIWISPFYKSPKYDFGYDISDYRSIDPDFGTEKDFKELIKEAHKRKIHIILDMV
                     INHTSHQHPWFIDSSSSRQSAKNDWYLWVDGKKGKFPNNWMSAFGGHAWHWSIERQQY
                     YLHLFLKEQPDLNWRNPDVQKAVFSDIEFWLKLGVDGFRLDVINYIVKDKDFKNNPYF
                     LHKTKPRRHDLQHHQHDRNQPETHIILKKLRKLMGNYGETMLVGEVYPDEDVMKPEIP
                     ASYLGNGKDELHLAFDFSTIFAKFSAKCFRKMLTRWYHAIPQDGWPCHVLSNHDKSRA
                     ISRLAKGNTDKAKLLMTLLLTQYGTPFLYYGEEIGMTDVPIKRKDIVDPPGKQYWPFY
                     KGRDGVRTPMQWNSKKNAGFSNSKPWLPVSKDSVHINVKYQYDKHDSMLNYTKALIRL
                     RQTLTSLNRGSINFESSHSSVLAYSRTHQSESVMILLNFSRFKKSYNLPSNDYHIVLN
                     SLITNTTIAVRNNIVLQPYQALIIKKENS"
     gene            14146..15222
                     /locus_tag="P5123_00060"
     CDS             14146..15222
                     /locus_tag="P5123_00060"
                     /EC_number="1.1.5.-"
                     /inference="COORDINATES: protein motif:HMM:NF019607.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016901 - oxidoreductase activity, acting
                     on the CH-OH group of donors, quinone or similar compound
                     as acceptor [Evidence IEA];
                     GO_function: GO:0070968 - pyrroloquinoline quinone binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PQQ-dependent sugar dehydrogenase"
                     /protein_id="HRX14685.1"
                     /translation="MLLKRLLIIFVLINSPFSISAADPKPQLMYKSNSVIWGFTFINA
                     NEIIISEKSGKLGLFMVNSRQYREINHNIPFSVHGQGGLLDVAYDSSIMTLFITYARK
                     TKTDLYTTALASGKYSENTNSINFKEIFVSKAESPKGVHFGSRIALDKKSRQLYITIG
                     DRGLRKRAQDLAFHNGKILRLTYNGDAVSTNPFYNKKNALPEIFSYGHRNPQGIYIRK
                     DGHIFISEFGPKGGDEINIILPGKNYGWPLATYGKEYSGATIAPTHHPGTEQPVVWYV
                     PAISPSGMTIYEHDYFPQWTGQIFMGALSARHLHRLNIIQGENNQKVKMYSELNKRVR
                     HVSYNDVGQIFFSTDHGELYIISK"
     gene            15449..15880
                     /locus_tag="P5123_00065"
     CDS             15449..15880
                     /locus_tag="P5123_00065"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRX14686.1"
                     /translation="MTGTMTAQSSIYLSKKLTAAAKKLGERELITLVRKAESLLDQQK
                     SFRDSAKTNPLSVTVSDSTDGNSFRLLIGSTPKVFQRYEMRKMVKLCQKADNRIRGSQ
                     KLKIWLRNNRPDFFLDHKIGNNWDLALAEIYDYLINNYTVQ"
     gene            complement(15984..17441)
                     /locus_tag="P5123_00070"
     CDS             complement(15984..17441)
                     /locus_tag="P5123_00070"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004776790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RecQ family ATP-dependent DNA helicase"
                     /protein_id="HRX14687.1"
                     /translation="MINETKSPEWILRNIFKKNEFRASQKKIIESLMNGKNVLSVMPT
                     GMGKSMCFQIPALIFDGLTIVISPLIALMKDQVDRLKKTGIDAEFINSSLSYDQRESR
                     YRGIKQGKYKLLYISPERFRNSTFLKSVSFRKISMLVLDEAHCVSQWGNDFRPDYAKI
                     FEYRAILNNPVTAAFTATATTEVRNDILSKIGVCHDDVDVYNDGICRDNLNLSVYQVY
                     GELDKFEAITEYLKFRKGSAIVYFSLISSIEKCCDYLINKNMNFIVYHGKMDKYRRQD
                     AQNKFIQSKDQIMIATNAFGMGVDKEDIRTIIHAEIPDSIESYYQEIGRAGRDGNSSE
                     CRLYYDESDLAVQLQFLEWRNPDQKFLKRTLSLLQNSQAALNSLSYQDIQEQLVFKNR
                     SDHRLATGLSLLERFGYIEGEPGNGTLRLKHNGDDSLFDLKRIDDKMKYDRIRLLGML
                     NYVKEKGCRKNYIHDYFSSEKIVCGNCDNCMNENL"
     gene            17797..18414
                     /locus_tag="P5123_00075"
     CDS             17797..18414
                     /locus_tag="P5123_00075"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane beta-barrel protein"
                     /protein_id="HRX14688.1"
                     /translation="MKKGILILLVLFSTSMLSALNLQKSAGLTLNATRYSWEYEYGDN
                     TSQASFPYSVIGIEAFFDMTYFRVGLSYNSSMGDITFETETDDSSTSQDLEYEFSTCD
                     VMLIGKYPIKKGKFDIWPGLGINYSMNMTFNDEDGNEIEDTDMDDLYILAVLGMDYHI
                     DSKLYITGSLDFGYNINPKMYDNSSNEDDWSGYKSSIKIGAGYYF"
     gene            18642..20771
                     /locus_tag="P5123_00080"
     CDS             18642..20771
                     /locus_tag="P5123_00080"
                     /inference="COORDINATES: protein motif:HMM:NF013188.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation:proton antiporter"
                     /protein_id="HRX14689.1"
                     /translation="MRLLTIFLYLIPSMAFASQDTHSANITHMMTKLVFEIGVIIFAA
                     KLGGITAEKLKMPSVLGELIAGIIIGPYALGIIVFPGFPDGLFPISTATALPISPELY
                     GFATIASIILLFAAGLETDFTLFLKYSVTGLFLGIGGVVVSFALGALTTTFFMNVSFM
                     SPQALFLGVMSTATSVGITARILSERRKMDSPEGVSIMAGAVIDDILGIIILAIVLGI
                     SAAATHSSGNSVDWSNIAFVAIKAIGVWVFFTFLGIVFAYKISRFLKMFKSVSTFSVM
                     SLGLAFILAGIFEKAGLAMIVGAYVMGLSLSRTDLSFVIHDKLHTIQLFLVPIFFTVM
                     GMLVNPATLFSREVLLFGTVYTAGAVAAKIIGCGIPSLILRFNKLGSLRIGLGMVPRG
                     EVALIIAGIGLSYGILDNTMFGVGIMMTLLTTLIAPPLLNLSLKSDKSGTRKQVEKSN
                     QITTTFATPSASLTKIIEDRILENFHEEGFFINLIVTNVNNYHLKKDAININLTVSRF
                     VIEFKTDEADLPFVKNVIYETLLNFKTAITEVQSIVDSLDMRKEIVGKGRTNKSEVRK
                     HLKHSNIIIGLESNTKEGVIKELVSVFKDFKEIGDPRDIISALLEREKVMSTGLADGI
                     AIPHAKTEQVKSIQLVIGLAPDGVDFESLDGKPSKIFFLILSPLNESSSHLQMMSNIT
                     SIFYEKANRKELLSCSDTQSIMDFFSK"
     gene            21003..22046
                     /locus_tag="P5123_00085"
     CDS             21003..22046
                     /locus_tag="P5123_00085"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRX14690.1"
                     /translation="MNTTEKIEIYDYDSDTLISEFDENSSIELYSESGKHINSNELNH
                     DTFTVRTSEPIQNETLDNDHYVKQNLSEPSAIEDEISINKNKTTSRTEATTVNSNIKP
                     ISAPSHDKETRILPLSRLMIRADKIAYAIINHKELDASFFIAPDETPLTQDEIFEIVN
                     QAEKLEFIECEGIGNNNDECFSNIYTENISQEVKNANKSESNSLKNVKKQNLYYSSHF
                     IEKPNLDALPKPGLSEIERTSIEEDIFTEHSLVIEESIEDIKQKLLEYWYMQCEQCED
                     ISDTIVILENDSDVERFINNFPDIAKKEKLKTLLEYFGNLFEKLPEDTLNSFAKSEYF
                     DMYIDFMNQLQKN"
     gene            22067..23638
                     /locus_tag="P5123_00090"
     CDS             22067..23638
                     /locus_tag="P5123_00090"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="GAF domain-containing protein"
                     /protein_id="HRX14691.1"
                     /translation="MEKNEQKLKDISDIITELDNTLSNDTIFLDLEEDSKSGNEQYIS
                     RKNLLNASDKIILDIAKEISGANSLSELYEILIFTAIGQLGISSASIIHPSTVSTERW
                     ILAEHHGMRLRNRNLTFKLNDTILSKAINKKEIIYLDDYQSDINAQEEYQKFFSIGAS
                     LIAPAIINENLMFALVVGSKLKQEEFSDNDIVFLNALLDITSVIFSKISQIDKLKNEL
                     KDLATFQKRLDMIDNYEADVRKTSTHIEIDNIIQKELSSLQINSYALFFRNDSTNMFE
                     ILFTEKEDLLHLKRKGYEITSSNAFISYCFSNDTYTEIENPVTSQILKTVFNDDFLLR
                     INVFGVYPYLIKKQLLGFLLLLRVNLDVYKTHINQVKRFSRFIFSHLQTSKYLIYSKS
                     FSDVLQPLLTRMRELQISCQNSRIPLTLIIIKIGGYSNYIQSVTLQQIALCNTQIESI
                     FDEKLQDGDYAFRFDIDRYIVALPGRKKRAGLGYSTILKRLIDEIEEEFTVITAVHEI
                     PKDGSAFEEFLKELS"
     gene            complement(23641..24531)
                     /locus_tag="P5123_00095"
     CDS             complement(23641..24531)
                     /locus_tag="P5123_00095"
                     /inference="COORDINATES: protein motif:HMM:NF013700.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing M23
                     family metallopeptidase"
                     /protein_id="HRX14692.1"
                     /translation="MKKTILFISVLLIIKSGLALEPGLKNLSRSSEEIKNLRAEIRTT
                     IATVKSNRDEKQIPELKFYKYRVKKDDNFWNIIARTTLNIDTLMTVNQLENPSDISVG
                     STIFIPNMRGIVHKVNKNETINDIGLKYKVNPRYIKYVNKINKLDKEFIFIPCGNLSR
                     EERSAFLSPGFRKPLDKTRITSAYGNRIDPFLRIPSFHSGVDFKCSLGTPVKASKSGK
                     ITFCGSNGNYGKLVIIKHTNGYETYYGHLSKFKVKIGDTVTAGQIIALSGNSGRSTGP
                     HLHFEVRKGGSVINPMTVLR"
     gene            24972..25406
                     /locus_tag="P5123_00100"
     CDS             24972..25406
                     /locus_tag="P5123_00100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002770963.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polymer-forming cytoskeletal protein"
                     /protein_id="HRX14693.1"
                     /translation="MALAKSGGDTANNTIGESSYFTGKFHINGSLRIDGCFEGKYLKA
                     DQLYIGPKGRLKTNIEAVSVIVEGLIIGNINASSRVLLMPTAKVLGDIRTPELIIQNG
                     VILEGRCTIANDLKSSARELIDKEYLKNRFVPNEFVESRKRA"
     gene            25375..26394
                     /gene="pdxA"
                     /locus_tag="P5123_00105"
     CDS             25375..26394
                     /gene="pdxA"
                     /locus_tag="P5123_00105"
                     /EC_number="1.1.1.262"
                     /inference="COORDINATES: protein motif:HMM:TIGR00557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050570 - 4-hydroxythreonine-4-phosphate
                     dehydrogenase activity [Evidence IEA];
                     GO_process: GO:0008615 - pyridoxine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxythreonine-4-phosphate dehydrogenase
                     PdxA"
                     /protein_id="HRX14694.1"
                     /translation="MSLSNPENVHRFIAITVGDPSGIGPEVTLRALDQINQTDFTTVV
                     IGNYELLKNLSVSLGIKKPLKTVDNDFFHAPPDNLKDIFIYDIDSHLPIPTPRFPSVE
                     TGKESLAYIDKAIELWKKSYISGIVTGPVSKNLIEKTGIRFSGHTEYFAQMIGEDTPY
                     MIMYSDKYSVILVTTHLPIKDLANHITKDKIIKTAIEAQRSMTLIRNKKPKIAICGLD
                     PHCGDNGAIGSFDTSITSPLVQELQQNGMDISGPFAADTLFLPSEWEKYDIVIAHYHD
                     QGLIPFKMMAFDTGVNVTTGLSLIRTSVDHGTAFNIAGQGIASFSSMIQAIKLCKSLI
                     RNKSR"
     gene            complement(26391..26786)
                     /locus_tag="P5123_00110"
     CDS             complement(26391..26786)
                     /locus_tag="P5123_00110"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRX14695.1"
                     /translation="MKNRIKFCVVTVFMFTSFVSVVLANDLQIRSESPNFSDKDTLLI
                     EKSLIKISGDYRAEVIILTMNNKSEIYSRNKDGNVVMEEKQAYIRFMITVLEGDDKKS
                     VKFIELFAPSRKDLVFRTIRTINHELKDI"
     gene            27285..27773
                     /locus_tag="P5123_00115"
     CDS             27285..27773
                     /locus_tag="P5123_00115"
                     /inference="COORDINATES: protein motif:HMM:NF025910.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HRX14696.1"
                     /translation="MKRFILLFLFIFSQIEAAENSTLLYYQGRELALNGDIEAAAEKF
                     KQSIKLSPYYVLPHYGLGRCYLNMEGKRKEAIRELRISIKLDKKFSPAYFYLGLAYMF
                     EKQYAYALNQFKNALLYDNTRTEALYNIGAVYDLMGHPNKSLLYYDQYLRAIKTDSDY
                     RE"
     gene            complement(27826..29013)
                     /locus_tag="P5123_00120"
     CDS             complement(27826..29013)
                     /locus_tag="P5123_00120"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRX14697.1"
                     /translation="MYKKLALLLLITALSLGTLLAQEEPEAEAEAVTSDYSSLEEVTT
                     DSPTADIGLDAVDDSMDDSADDVAPVSGIDTVTEPTMTDTYPSTSTATTTTTVAKTYF
                     DGVNTYANSRVQFKLHTSDNFLADKLIYTINDAGENDYLSPFSIPDEGRHNIKYWGVD
                     SIGNVETPKVFTIYIDNTAPESIVTLEKPVYEIGGKYYISETNSISISTRDAMSGVNN
                     VQYSVNGTDFTGYSTSFNLTTEGDLDFRVRSEDNVSNISEEFRVYLPDESGTPTSMTK
                     SSLSFYVDKTAPTVDITADRPFIERKGKKVAGTDYIYTINANDTESGVREILYRIDGT
                     GAFRKYDGTFTLPTNGDHFIEAQAIDNVGNKSEIVILSIFVDVIPPSSGITVVEEEEV
                     VTE"
     gene            complement(29239..29682)
                     /locus_tag="P5123_00125"
     CDS             complement(29239..29682)
                     /locus_tag="P5123_00125"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRX14698.1"
                     /translation="MKQLRISVLLLLILLSTKIQADEFETIVRLSYYSKPVSTLEWSV
                     TVFRSGHTAYVQIENFRSNTRKRSIHPERYKKFITIMDREHLLNIDSDDRIADESCYY
                     KLYYKYRGVSKAVVIDPLKPHIDKKKGIPVIIRTIINYGILIMPE"
     gene            complement(29926..31122)
                     /locus_tag="P5123_00130"
     CDS             complement(29926..31122)
                     /locus_tag="P5123_00130"
                     /inference="COORDINATES: protein motif:HMM:NF023568.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipase"
                     /protein_id="HRX14699.1"
                     /translation="MLNYSHEEGRITGKNDIELYHQSWVSPKEKALLIIVHGLGEHCG
                     RYNHLVEQLNGKGITIYGMDLRGHGRSGGKRGHVDSFLDYVHDLKSFIELIKEKHGGL
                     PIYMLGHSLGGTIACRYALQYQEDLSGLIISAGAFIPKIEVSPFLHNVSMFLSKIVPG
                     MQRSNKLDVSFLSHDEEVAELYKADELVHDRITLKWYCEYNKNAENCLGRGMEIRLPT
                     LILHGGDDRIADIKGSKLLFDKIMSTDKEMKVFEDLYHEIMNENGKDRSKVLTTISKW
                     MSERVAAASKMIKSDKKSTQTKSAVSASANTEKKELKKPATKKAAKKSASKPAVKKVA
                     KKAAKKAAKKATVKKTAKKPAVKKSAKKAVKKAVTKKAVKKAAKKPVAKKATVKKTAK
                     KTVKKK"
     gene            complement(31418..33229)
                     /locus_tag="P5123_00135"
     CDS             complement(31418..33229)
                     /locus_tag="P5123_00135"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HRX14700.1"
                     /translation="MDFSVLHSLSVPCCIINSSGEIVFRNESFTVLTGSSESSELTLD
                     DSHELFNEYRKKIALNFSKTLSGLDTRFFVVLKGHNNKKIPAELYLYPMLIENERSIA
                     LIIRPVEDRVVSFDRSMQNPNEHASLYDYSPCPVVTVDTSARLVRISSSAENYFGTNR
                     DFALENPDCFLSFFTSYCSEKLKAAVVDICNGSKSFVRINDLKVNTKTGVENSCNAAL
                     YKIDSDINGHDIEIVFEDTTRLKMLESKLSKMNRVQIFSDMTKGLLHSFNNLINVIIN
                     RSQMLSQLTDKPNILDGLQSINESAVDAATQIRRIQEFITSDSVEYNQGSVEIIELIR
                     DAIEFASIHFKVERKEKGRSISISKLYYTKARVAGNLKILREIVVSMIFRISSYIGQS
                     GAVDIELKKDDDIIFTVSVKRGLVDNDPLETGEDFLPEIELRRIADKINVRIIEEESA
                     DIISLSAVIPAKMIVTGEDSASNSDKQKIRDCDILIVEDEFALTEILYELFDFMGNRV
                     IVHEDAESGLNEFKNGHFDIVISDYGLKGMTGTELLTEIRELRETTLTVLLTGWMIED
                     IKSYNKQIDLFLQKPFQIEVLLNQIALKLAEKKNKLK"
     gene            complement(33394..34797)
                     /locus_tag="P5123_00140"
     CDS             complement(33394..34797)
                     /locus_tag="P5123_00140"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRX14701.1"
                     /translation="MKNKCITIIAVLAYLLLYGCSSSYSSKVNDAESRFYGGDYLAGA
                     DMLSTLAKAESNERLLYMIEAGLMYHTAGDYKKSNEFMLKAASFAQDQKISIRKQAAS
                     VLLNERQGRYHGEEFERVLIHMYAGINFLMLKNYDSARVEFNRVSRRLDELRKNGESL
                     YKQNIMARYLTAICYEIIANEDHDEGALEFAYIEYKRIYEMRSDYEPVHDDLLRLAHI
                     MNDKEDLAKWKQKFKQKAVPSYKDKGELVIIHQTGRGAYKKSRGSLLADSNMKTAIIV
                     SVNGMTLEQGVTLAAIMVALGSIEHPIPDYISPGNKTAYLNVRTIDGKVLGKTQTLED
                     IEKTARLSAEKRYEDTREKVAMGIALKAVATVASSIAAEKIAEQTPLKDAAPLIGLLT
                     GAGVGSALASQIKPDLRCWRTLPSNLQISKIFLEPGKYTLRFEQVDANGKVFATEDQE
                     VEILKNSKTIINKRTLR"
     gene            complement(34804..35388)
                     /locus_tag="P5123_00145"
     CDS             complement(34804..35388)
                     /locus_tag="P5123_00145"
                     /inference="COORDINATES: protein motif:HMM:NF024436.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRX14702.1"
                     /translation="MKKYLICLTVLTISISSCVSAPEVVYEREVTEDHGSREWGALEV
                     KLVVEKMVESLYVFLKEDWNGPAYLQVKKFRNRTAEHIDTGVVTDEMTTELIKRRIKF
                     LDESLAEDSIKEMEKGMTGLYDQSTAVPTGFLKSPNFYLSGDIREAVSTRDGREIQLL
                     VVTMRLYNIKTGEIHWQENARFLKSKSKDKGKEF"
     gene            35723..36952
                     /locus_tag="P5123_00150"
     CDS             35723..36952
                     /locus_tag="P5123_00150"
                     /inference="COORDINATES: protein motif:HMM:NF024873.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease H-like domain-containing protein"
                     /protein_id="HRX14703.1"
                     /translation="MDIASKLGIYKKKSQPITENATKKSIIFPDESIADKLSINGGTV
                     WRFENRLSLFDVYSPVNISPIRELDAFLEINGFSNDFSLSDLLFFDLETTSLSIASGN
                     YPFVCGIGYTDGDDFVTEQLFMESYSDEKAILTYLLDFFNRSKAVVTFNGNSFDLPLI
                     KNRYMLNRIYGFPVNIPSIDLIVSSRRIFKSLYESCSLQTLEKNILGFERIDDIPGWM
                     IPEIYYTYQKDGDSSRLPGVIEHNMYDIFSMYILLQILSSIFSDIKKGNFRNFATSSL
                     FTIARCLFVNNTELFLQLAEYLGTDLFGDDTVFEKFSIVLKRNKEWYKAVDYWNKSGS
                     FFSLIELAKYYEHKEKKYIQALNVSNKANEILSKKETNKDENPDKVKKRRDLLQVRIN
                     RLKKKISASNNGTNPSL"
     gene            complement(36923..37084)
                     /locus_tag="P5123_00155"
     CDS             complement(36923..37084)
                     /locus_tag="P5123_00155"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRX14704.1"
                     /translation="MAAIPAESFTFQMGNISFQVERQNAEMFPGVEGEMIVIEYEIEE
                     SEAGVCPII"
     gene            37464..39902
                     /locus_tag="P5123_00160"
     CDS             37464..39902
                     /locus_tag="P5123_00160"
                     /EC_number="2.4.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008180653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycogen/starch/alpha-glucan phosphorylase"
                     /protein_id="HRX14705.1"
                     /translation="MDTESIRRGIGTNLVYSLAKDRFSATDRDIYESLALTTRDRLIE
                     RWIKTQQTYYDKDVKRVYYLSMEFLVGRTLGNSLINLDMMNQTSDAVKQIGYNLEELR
                     EIEFDAGLGNGGLGRLAACFLDSMATLAYPAYGYGIRYEFGIFKQKIRDGYQVEYPDN
                     WLRYGNPWELGRPEYLFPISFYGNVEYVPDTENTAVKKWVNCQKVYAMAYDTPIPGYK
                     NNTVNTMRLWAAKADREFDFLHFNEGDYNQAVANKANSENITKVLYPNDNTMQGKELR
                     LKQEYLLVAASLQDIIRRYKKYHDSFSQFHEKTAIQLNDTHPSLAIPELMRLLVDEER
                     LDWNSAWDITVKTFGYTNHTVLPEALEKWPVDLIKRVLPRHLEIIYTINMKFMELIKE
                     KYKDDAKRMAAMSIVEEGEYKNIRMANLSIVGSHSVNGVAALHTEIIKNSCFKYFYEL
                     WPERFNNKTNGITQRRWLKLCNPKLAELISSRIGENWVTNLFELKKLEDHINDSAFRE
                     SFTSIKKQNKIDFASYIEEHTGIKVNPDSIFDCQVKRIHEYKRQLLALLYAITAYHRI
                     KDDPSRDFVPRTMIFSGKAAPGYHMAKLIIKLATSIGDVVNNDPDVNGRLSVVFVENY
                     SVSIAERLIPAADLSEQISTAGFEASGTGNMKFALNGALTIGTLDGANVEIKEEVGDD
                     NIFIFGLTADEVESTKYSGYNAREYYHKNGELRRAVDAISNGYFCPSMPDLFKPITDA
                     LLNHGDPYMLLADYETYINMQEKVSTAFTDKEKWTTMSIYNVARMGKFSTDRTIREYA
                     EEIWKIDPVSVKID"
     gene            40120..41151
                     /locus_tag="P5123_00165"
     CDS             40120..41151
                     /locus_tag="P5123_00165"
                     /EC_number="4.6.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012437.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009975 - cyclase activity [Evidence IEA];
                     GO_function: GO:0016849 - phosphorus-oxygen lyase activity
                     [Evidence IEA];
                     GO_function: GO:0032555 - purine ribonucleotide binding
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate/guanylate cyclase domain-containing
                     protein"
                     /protein_id="HRX14706.1"
                     /translation="MKQNEISHLKQYIFFLLLSGLGGGFFGLVIPKYSDPQYSTALSF
                     CIGFLIGFCICVLAILFDNKFKIIIRKYKVPLLLELSITTLYYALIIPVVFYSIFYIF
                     RLQEEISTLLFPVMVYSLGASFFINMLAIIINLIGPPDFLRLISGRYSRPFEEERIFM
                     FIDIKGSTSLAENLGNLKFHSFLNDFIFFITPLILECKGEIYKYIGDEIIISWNPKKG
                     LKHLNCLRIFFLIVNAIQDKKSYFETEYNNVPEFRAGIHIGKVIVGEMGDIKKEIAYL
                     GDVVNTTARIVNVASERSIPLLGSAEIVNFVSNNKLKTDYLFTEIKEVMLRGKSNQSA
                     VYQITENIM"
     gene            41188..41946
                     /locus_tag="P5123_00170"
     CDS             41188..41946
                     /locus_tag="P5123_00170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013007836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HRX14707.1"
                     /translation="MKKKIEIQKLYKAFREKKVLQGIDLDIFEGEIISVVGKSGTGKS
                     VILKNLIGLMKPDTGKIIVDNEDFTQSSGDNFYRIQQKFGVLFQMAALFDSMNIFDNV
                     AFGLRRKKNDESFIKKRVPELLEQVGLKDIETKMPSEISGGMQKRVGLARALAMNPQI
                     MLYDEPTTGVDPITGSAVDKLILKMNNAFNITSIVITHDLESAFRISDRIAMLHEGNL
                     IFLGSPDQYKNSNDSVIRQFSAGQANPSEQTEIQ"
     gene            41946..42809
                     /locus_tag="P5123_00175"
     CDS             41946..42809
                     /locus_tag="P5123_00175"
                     /inference="COORDINATES: protein motif:HMM:NF021445.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidylglycerol lysyltransferase
                     domain-containing protein"
                     /protein_id="HRX14708.1"
                     /translation="MHSEPLDLHHKEILYNRLRAISIRLSEFSFANLYLFRKTHGYEV
                     VHDGDCIHITGFTGDKQKFVLPLCEKKEPDLEHIDELLDNYEMIFPVCDCWLRYFDPR
                     NFDIHHNEDDSDYIYTVEKMATYSGRHLSKKRNLLKQFNNSHQYTRQILNKDNIEIAI
                     KVLTNWQDETPADASDTDFHAAMEALENMEILGLFGFLYIIDDKPGAYVLGEDITDDT
                     FVLHFAKGLTEFKGVYQFMYNDLAKEIQGKYNYINFEQDLGLQSLRQAKSSYKPDHMG
                     MKYRIKRRRCN"
BASE COUNT        14144 a         8370 c         6932 g        13476 t
ORIGIN      
        1 acactaaaga gcatctcgcc gtaagttggc aaaggccagt actcggaatc aacaaggagt
       61 tccagacgat caaaatctaa acgaagatca ttcattgccg ttacaacatt atccctgaag
      121 gctgcggcct gttttggcac atcatcaata gcgagagttt gttctattgc cttatcaaga
      181 acagctattt ttttctttgc cgatgaggca agatccgaca cttcaattat taaggattcc
      241 tgagcttcaa gcgtagcagc aataccggta gcttttacag cgttaattga atgtgccaaa
      301 tctgtaataa atctaaacgt cgacggtaaa agatcccttt ttgcaagctc taacgcagtc
      361 atagcctcta ttttaatcgt gttaacatag tgttccaata atatctcata acgggcatgg
      421 cactccctct cggataacac ccccatgtcg ctaaacattt ttacattttt tggggcaatc
      481 aaagccagtt ttgcatccac aagatttctg tggtgaggaa gccctctttt ctcggcctct
      541 ttaacccagt cttccgagta tccattacca ttaaaaatta tacgtctatg ttccttcata
      601 tattcaacaa tgattttttg aacttcggcc ttaacatcag aagcactctc aagcctatcg
      661 gcaacttcgc taaagacttc ggcaattgca gcgttaataa caaaatttgg tcccgcaatc
      721 gactgtgaag acccaggcat acgaaactca aacttattac cggtaaaagc aaacggagac
      781 gttcggttac gatcggttgt atccttgtcc atgtcaggta aagtcgaagc accgaggcgc
      841 atctgctcag cagagataca agatgaagcg cagcccttct gaacgatctg atttataatg
      901 tcttcaagtt gagctccgac aaaaattgaa ataatcgcag gcggagcctc attagcacca
      961 agacggtgat cattacccgg tacagctgcc gaagatcgaa tcgcgtccgc gtatttatca
     1021 acagcacgca ttacaaccga aagcatcaac aggaattcgt catttttatg tggagcgttc
     1081 ccaggatttg ttaatagacg gccagtgtcg gttcccatag accagttatt gtgtttaccc
     1141 gatccattta caccggcaaa aggtttctca tgaagcagac aagccagatc atgacgtgcc
     1201 gcaacttttt taatggtttc cattaaaagc tgattctgat cggtacccac attagttgta
     1261 cagaaaatag gcgcgatctc gtgttgtgcc ggagcgactt cattatgtct tgttttagaa
     1321 ggtattccca acttccaaag ctcctgatcc aggtcgatca taaaatccat aacacgtact
     1381 tttatcgccg taaaatactg atcatccata tcttgaccct ttgaaggagc agcgccaaaa
     1441 agcgttcgtc cacaatactt cagatccttg cgtgcatcaa acatatcttt atctataatg
     1501 aaatattcct gctcggcacc gacataggta ataagttttt ttgattcatt atctccaaga
     1561 gcacgtaaaa cacgcatgcc ctgtttgttt atggcatcca tcgcacgcaa aagaggagtc
     1621 ttcttatcga gcgcctctcc ggtataagaa gcgaaagccg tcggaataca gagtgtattg
     1681 tccttaacaa aagctggtga agtacagtcc catattgtat aaccgcgtgc ttcaaaagtg
     1741 gctcttattc ctccgtttgg gaaggaagaa gcatccggct cgcccataat caattctttt
     1801 cccgagaact caagaataac acggccatca cctgttggcg aaataaatga gtcatgtttt
     1861 tcagcggtaa taccagtcat tggctgaaac cagtgagaaa agtgagtcgc tccctgctca
     1921 agagcccaat ctttcattac actcgcaact acctctgcga catcaggagc caacttttca
     1981 ttattatcta tcgttttttt aagcgccttg taagtggcct tgggaagcat ctcacgcata
     2041 acagcatcat taaaaacctt tactccaaaa atattgctaa aatttgacat attcagcacc
     2101 tctaacctgt aaatttctac ttaaagcttc aagcaaacat gatgtaccta tcaactttaa
     2161 ccatgttttt cgcaatacca ttttatactt tttggtacaa ttaagctgtt atttaacatt
     2221 ggtttaataa ttgctataat tatgcccgat tttaatatga tttgacttgt atgaattaaa
     2281 catctttata tccgttgtta tccatgagat tttaaacaag tgattaacat caacctagat
     2341 ttaaaaatga gaggagaggt tcttaatcta tagattctca cagtttatat caaaactata
     2401 gaatgaatta aatgcttgag aagccttgtc ttttatttat gatatgcgcc ttggcaaatt
     2461 ctgaaatatg aaataactac tgtttatgct tattaaattc ctgaaaattc atcaaataaa
     2521 caactttttt tcttaaaaat aaagagcctc cataaattta cagaggctct ttttttgatt
     2581 attatatact tttacaacac ctttcaatca caacaaatca atcttcaaca ggataaccgc
     2641 taaatttttc agtatattca gaataggtta taacaaaagt atacttttta tctttctttg
     2701 gcgcttccag atattcctca tagatgtaag tctcataaac cccctcgata gctacatcaa
     2761 attgaagaaa gcctccctcc agtttcaaat ctttatattc tgatatagta ttagcaatca
     2821 cgtctcccat attttgatca gcaaatttaa cattgagaaa atcataatcc gtcatatttg
     2881 atatacgacc ttgagcctta tcctcatcat cctcacaccc agcaaagctt aaaacaaaaa
     2941 acagcaaaaa cagaatcact cctaactttt ttaacgcaac tttcatttcg ttaatccccc
     3001 tttattttga cttaaggaag caacaaaaaa ataattctca cgttagcaat tcagcacaca
     3061 ccgtttaaga acttattaaa acacttagca gttatttctt catattttcc ttagttgtaa
     3121 gcaattttta taaaagcaat atataacata atctatatat aagcagtatt tatttattgt
     3181 caaccgacat cagctaactt tttaaaaaaa tttgaatgat tttatactct acacgtcatc
     3241 tcgcatatat gccgcataac gtcttttcat tgattttaat tagtactaac gtgtggataa
     3301 aaattacaca ctcaaaacta ttttaaaata atacttcaac aagaaagaga aaaaggttag
     3361 ttttttattt tacaaccagg agttttagtc tgccttaatc atatttattt cttccagcct
     3421 ttttattgac aagcaaagct caataacaat cattgatcta acaaagatta tactatcctc
     3481 ttatggaaca gatatgcaca aagccaatca attattaaaa gaaatattcg gttactcctc
     3541 tttcaggcct gttcaacaac aggctataaa gtctgttctg gacaaacatg acacgatcgc
     3601 gattatgcca accggtggcg gcaaatcact ctgttaccag ataccggcac tgatctttaa
     3661 cggcttgact cttgttgtat caccgcttat ttcattaatg aaagatcagc ttgatcagct
     3721 tttagaactg ggaatcgaag ccggaatgct taacagcact ctaagcgacg atgaatacgc
     3781 gcaaacagtg tcacgaatca aagaaggaaa aatcaaaatc ctctactgcg cacccgaaac
     3841 ccttttgcaa caaagaacaa ttaacctgct ggacagtaca aaactatctt taatagccgt
     3901 agacgaagca cactgtattt ctgaatgggg tcatgacttt agacccgaat accgccgcat
     3961 tgctgaagtc aggtcgcgtt ttaatgacgc agtatgcatt gcccttacgg caaccgcaac
     4021 agaccaggtt cgtctggaca tcgcatcgca actaaagata aaacccgatt cggtcatggt
     4081 agcctcgttt gatcgaccta atctttttat tgatgttatc cccaaaacag atccacagaa
     4141 gcaggcaatc gactttatac agcaatttag cggacaacct ggaattattt actgtttctc
     4201 aagacggcaa gtcgatgagc tctcagagta tttaaataat aatggcatca ataaccgtcc
     4261 ctatcatgct ggtcttaccg ataacgagcg gcaaacaaat caaaaccttt ttcgaaacga
     4321 tgatataaac attataacag caactgtggc atttggcatg ggcattaaca aacctgatat
     4381 tcgttttgtt cttcattatg atatgccgca aaacatagaa agctactatc agcaaattgg
     4441 tcgagccggg cgagacggcc ttcctgccca ttgccgtctg ctttttggat acggcgatat
     4501 aaacaagatc cagtttttta ttaatcaaaa gaatgactca gaaaaaaaaa cagcttacaa
     4561 caaattgaat gacctattga gctttctcga aaaaagagag tgcagacgcg tgcctcttat
     4621 gacctatttt tcagaaaaac cgtcatccca aaaatgtgac aattgcgata attgcttaac
     4681 tcctcctcct gatgaaaagg agtacactat tcaggctcaa aagttcttat cttgcataat
     4741 aaggcttgat caacgctacg gagcttcgct cattattgat gtgctgcgcg gctctaaatc
     4801 agaaaaggtc aagaaatctg gcgcattaaa tatatcaact tatggaatcg gatctgagct
     4861 ttccaagtct gaatggatga acctgtttag acagatgagt tctaaaggtc ttttaaaagt
     4921 taatacagaa acaggctccc taattgtaac aaaagtgggt gcggatattt taaaaggtaa
     4981 acaaaaattt tatggatata tcacaccggc tgcatcacca aaagcttccg taaaaaaaag
     5041 ttacctgcaa aacagtgaac ttttcgaaca acttcgtact ctccgaaaac agatagccga
     5101 tgccgacgga gttcccccat acacagtatt tcctgacaaa actcttatcg agatgacaca
     5161 gtttcttcca caagatccag atgaccttct tatgatatac ggagtgggtc acaacaaact
     5221 gcaaaaattt ggaaatcagt ttttagatat aattaaaaaa ttctgcatca ataatccaag
     5281 caacaaccac caaagaatta cagcaaataa taagaacagg aaagaatcag cacacagcat
     5341 aagctccaaa atgtaccaag ccggtacacc tgtccatgaa attgctcaaa gattatccat
     5401 ccagaccagt acggtaatcg accatcttta taaatggata tccgaagata accctctcga
     5461 ctgtgaggct cttttagacg agtttaatat ttcggaagag ctctgtttca gagttcactc
     5521 tctttttgaa aaacatggtg ttttcaagtt aaaaccggtt tttgaagaag ccgcaggcga
     5581 gatagattac gaaatactga aaatattgcg gttatggtat attaccggca aaaactcata
     5641 atagaaaact tcgaataatt accttttctg gaacggctat gtcgtttaaa gccaaattaa
     5701 agttattttt taaagactgc caatagttcc caacatataa tcataccgat aagtgacaat
     5761 tatttcctat aattaaaatt tttgaatatt agcgtagttt aatataaaaa tacttgaaag
     5821 ctgtttcgaa ttatctaata tatacaccat tctgctccat aaaggtataa aaatgaaatc
     5881 tctaaaattt gtaaaaattc atggaatcgg taacgactac atttatatag acggaatcaa
     5941 ttcaacaata aacaagctct ccgacttatc aattaaaata agcgatcgcc attttggtgt
     6001 tgggagtgac ggattaatag ccttacttcc ctctcaaaaa gcagatttca gaatgcgtat
     6061 gtttaatgcc gatggaagcg aggccgaaat gtgtggtaac ggcatacgag ggcttgccaa
     6121 gatgattcat gatcacggct tttcaaaaaa gaaacaactc actatcgaaa cacttgcggg
     6181 aatcatggaa ctggaactta ctctaaaaga caacaaggtg tccatggtca cagtagatat
     6241 gggagaaccc attcttgaaa gagaaaaaat cccgatgata ggtgaacccg gccatgtaat
     6301 cgaagaaaca atcgaactgg aagattctgt caagtttgag ttaactgctg tatctgttgg
     6361 aaaccctcac gcaataatat tcgttgaaga tgttgataat tttcctatcg agaaatatgg
     6421 cccattgatc gaaaagcatc cgcttttccc ttcacgaata aacgttgaat ttgttaaaat
     6481 cttatcttct aacgaagcaa aacagcgcac atgggaacgc ggttctggtg aaacaatggc
     6541 ttgcggcaca ggagcaacag ctgtaactgt tgccggtgtt ttgaccggtc gtttggacag
     6601 tcagtctacg attcatcttt tagggggcga tcttatgatc cgctgggata aacagaccaa
     6661 ccatctcttt atgactggtc ccgcaacaga ggtttttaaa ggggagtggt attactgaca
     6721 actattgaag ttttcaccac attttattct tctgaatcag aagcttcacc attttgataa
     6781 atagttttat taataactgc acccgattca ttataatttt gccaggtacc atgacgtttg
     6841 ccttgtttaa aaaaaccttc caaaattcga ttaccctttt tatcccagta aaccgctatt
     6901 ccatggtaga ttattttggt atcatttttg aaggaagcaa tttcgcaaaa cggttcacca
     6961 ttgcgatacc agatctttgt aaaacccgac ttctcactga actgaatcca tatatcgtct
     7021 ttttcattat aaactgctga gggcgggata ttgcccggcc tctgaaacga aggaagcaac
     7081 attattttaa tttcatgctt tacggcttgt ccgttatcat attcaataac atcaagcaga
     7141 cgcccccgag aatcataacg cgtcattttt ccatgaagat agccggcttt atactgtgcg
     7201 gttatcgcgc gagaaccgtt tttatagtaa ccagaggcat taccttcaag gtatccgttc
     7261 ttataattta taacagccag ccgccctcca ccgggataaa aacggatcca ccttccataa
     7321 aaaagatacc cctcctcttt tttattcgca ggcccccgag ctataatatt tttttcacta
     7381 ttatataaag ttaatacctt atttgactcg aatttttcat atttagaagc cagatccagg
     7441 cttttaaacc agagagggta aaaatctgta aaatcacgaa ttaatgacag ttgacgcgaa
     7501 tcagcgccaa ttgctgtaat agaggggata aataacaaaa agacaactaa agcgaaataa
     7561 ttaacaaaac cagacagctt aataaaggtc ttactcattt attattgtct tttcccaaag
     7621 attacggtta gaaatgccat tttcaaggaa agccacactc tttatttcat tataatttgc
     7681 ggtaatactc ttttcaacag cctttttaaa attaccttca gattcaggca aaataggagt
     7741 ctcgggattc aaagtttcaa gacgaatatc aatataacac tctgagccat gaatccacac
     7801 ttttctgata ttaaactcta tcggaacaac cattcttgtg ttttcatact ttgatccgcg
     7861 tactatttct gctactataa actcaatagt tttttcttta gaattttcaa gctctactaa
     7921 tcgagtctct tctaaaattt cgccttttac tcctggaaga tatattgtaa tatcttcccg
     7981 tttttccatc acaggaaatg aaggataaat atcaaaaata tttttttcaa catggaaaca
     8041 gtataaaaaa tagttcaaaa tcaacatcaa cgaaaccatt aaaagaaaaa atcgattacg
     8101 ttcacgcaaa tcacgacgta taagaatacg ataatccttg attttattca tgataacttt
     8161 ataatatttc atctataact ccagagcggg aaaaaccctt catttttagc aatttaatac
     8221 taaataacat ttgcaaagcg aatttattgt caaactttta tttattcttg aaaaatatgc
     8281 ctctaatttt atagcctgat ttcaacacga attggagcaa gaacatgaaa aaagacatac
     8341 tgccaaaacc ttatcagata cccaaaatgg gtacatccgg acttagactc aaacaggaac
     8401 aatataatag agccggcttt cttgagcaat ttaccgaagg cattgccaga tatttcaaag
     8461 agataacatc tactacaaaa attgctgaaa acaatacaca tctgcttctt ggaggagatc
     8521 caagagaggg caacagcgaa cgtattaaaa caatggcttg tatactaaca gcacacggtt
     8581 ttacagtcat tgttcctgaa aatgggttgg catcaacccc tgccatgagc cacgccatta
     8641 gacatcttaa aactatcgga gcaataatcc ttacagccag ccataatcct tttactgatg
     8701 tgggaattaa aattaatact gcaaatggag ctcctgcatt aaccgactgc accgagcgca
     8761 tcaacgagtt ccagaatgac gttcaagatt atcttactca agactacgaa actgctctaa
     8821 cagaaggatt aataaagacg atagatatta tttcaatgta tgccgatctt cttgataaga
     8881 tgttcagttt caaagatatg cgcgaaaaga ttgaagacaa ccccgtttcg gccgtttttg
     8941 attctatgaa cggagcagcc gggcctttcg cgcgtgaggt ttttgttaac aggcttggtc
     9001 ttgatccttt aatgcttcgc gaagaacctc gcaccgacct tgggggattt gatgacaaag
     9061 gtgaaccaaa acatcctgag cccgatttcg attatgtatc cgatttaata aaccataaca
     9121 gcaccaacca gttttccatc gcagcagcat gggattctga tgtagacaga cgtcttgatg
     9181 gtggttcagg tttctttctg gaatctgcgg acgaatttgc gatatttgcg cgttacagcc
     9241 accttattaa catacaatcc atgtttaacg acacaatata cttttgccga tcaactgtta
     9301 catcacatgc tatcgacctt atgagtaatg agctaacagc aatattcaaa gataaaacta
     9361 taaaaactgt tcagactcct accggattta aatggatcgc ggaattaggc aactggggag
     9421 tagaagagag caacggtgtt ggaaaccccg cgcttagaga aaaagacggt atttttgcta
     9481 cggttttttt attaaaaata attctggaaa caggaaaaac ccctctgcaa cttatggaag
     9541 atatatggca agatcatggc cgtgtttact ttacacgagg agaagtatcg ggatctgatc
     9601 ctattgaaaa agaggcttta atcaatattc ttaatggggc gcaatcgcaa atcggaaaca
     9661 agttcggcac gctaaccctt gaaaatgcgg gttcatggga ttataaacat cctgaaactg
     9721 gcgaaattgc ggaaaagaac gcagcctatt attttgaatt tagcgatggc aacagcatca
     9781 aggcaagatt ttcaggaaca ggttcaggcg gatacaccct tcgagtatat tgctcaaaat
     9841 ttgacaaaaa ctactcaatt cccaaaacca caatagttca acctatgaaa gatgcgtttg
     9901 atgcctttct tcaaaatggt ggatttaaag gtaaaagtga taaatataca gatagcaacc
     9961 agcccgatat ctattagaag ggaacttcga aaaattacca tttccagaac ggctatgccg
    10021 ttaaaagtcc attcataaaa gaatttttcg catttcacat gagaaaggag actctaactg
    10081 catagtctcc ttctcatttt aaacataatc aaaaaaatag tggctaacaa acccgatttt
    10141 tctaaaaaaa aattcaaatt ttcaattttt ttcagtctat aggtatataa actatactgg
    10201 agaaataaaa tggaaaaaaa cgacatccgc acatcaatcg atgtaggcac aaaatggctg
    10261 gaaaaaattg aagaatactc aaaggccaaa aaaataagca tttcagatgt tattaacagc
    10321 gttttggaca aatacatgaa ggagttggct gataaaccgg agacatataa ccgggcaatg
    10381 aagtaccaag cacgcggaac cgattaccaa tcggtacata tcagactgtc gtttatatca
    10441 tacagtcgtt taagagatgt aatgcttgtt tttcgttata gtctttcata tatgctatca
    10501 ttagcaatgg aattttttga tgaatatatg aacgagagtg aaaaagaatt ttttccaccc
    10561 tcattccact gtagtattat ttctagtact tctgttccca agtttttctc taattactgg
    10621 cactttccac cagaagagca cttactttat gaaaagccct ttgaagctgg ctaataatca
    10681 gaagaaatca ccatacaacg acaattttta ccacatacgg ctcatataag ggtaaggatt
    10741 gatcggaaca ttaccaagac gaacctcata atggaggtga ttacccgtag aagcaccggt
    10801 atgacctaca taaccgataa cctgcccctt ttttacataa tcatcagttt ttacaataat
    10861 actggactga tgcgcgtaaa atgtcttaaa cccatattta tgctgcacgc gcaccatctt
    10921 tccgtagccg ccaccccaac cgaccatgat tactttacca ggtgctgtcg cacgaatagg
    10981 cgttccctct ggtgccgcaa tatcaatgcc ggtatgaaaa tcccttccag ttccaaatgg
    11041 agatcttctc caaccgaaaa gcgaggtaat gtggcctcta ttaggtatta tcgaaggagt
    11101 atcgttgatt acctgacttc gcacatcaac aaaatttctt actgttttaa cggtatttgt
    11161 cgcgcatgca atatcgcttt gaagcttttt taattcaaaa acttctgatg gaagaacctt
    11221 tttcaaatga gcaagttcct cttctgaaac agcatcatag ttctgcacca tagaccatat
    11281 ttctgaaata tcctcagaac cggcagccat ttcatacaaa tcctctattt ccggacgaat
    11341 ctcagacata agctgagaaa cctcttttgt catcttttca taacttttta actgattatg
    11401 aatttcccga taattttcaa ttaattgttc ttcttcgctc ttaatctgat tatttttcat
    11461 ataagcaaac gacgaagtcg taacaactgt cataaaaagc acaacgaaaa atgaaattgt
    11521 aaatttggat atctgaaaat taaatatttt cttctcatca tgcggtataa acataatggt
    11581 cattttttcg tgcccatgtt ctaaaaatga tgaccacttt ttggcaattt ttcgtcttag
    11641 aacatgccgc ttctcgacaa tttttctttt tacggaatac agcataaatt gccctccctt
    11701 ttgtgaaatt attacatctt ttaactaaaa aatggtgtaa acttaattaa cccttcaatt
    11761 aacacttttt ggcaagtttt ttccttatat atttcaaaat tttatctctt taaggctttt
    11821 attccaaata aaaccggtaa gtccttaaag ggcaacattg tgattatctc attaaatcga
    11881 ttcaaatcaa taaaagcgct tccattttcc cattcaacat aagcccaatc atctggtatg
    11941 tctgcaaatt ttactttggt caaagcaaac ggagcaccat cattaaccgc tttttcacta
    12001 cttttttcta tttctttctt tattgtcggt tgataaccaa catttcccca cctacgtttt
    12061 gcatttgcca atttataggc actatcttta tactgtatat agttaaacag atacggaatt
    12121 ttttttgatc gataacttaa caggccgatt tttctttcag atcgagagcc acggctctca
    12181 taacggattt tataaacaag ttcatctttt accaactcta tttctttttc ccccgaagcc
    12241 aatcgatcac ttttgttttc tgtccaaaca aaaaaaaccg aaaccataat cattgataaa
    12301 accaacattc taagactacc cataggcacc ccatcatttt aacttactta attatcaaat
    12361 atttgacata ttatacaaac agaagtttca ttgccatcaa aaaaagattt gttagttact
    12421 aggagatttc aatgaaagaa aaacatattt ggtggaaaca cggagttatt tatcaaatat
    12481 accccagaag tttttatgat tctaataatg atggcattgg agacataaaa ggaattactc
    12541 taaaacttga ttatctaaaa gaacttggaa tatcaggcat ctggatttct ccattctaca
    12601 aatcaccaaa atatgacttt gggtatgaca tttcagatta ccgttcaatt gatcccgatt
    12661 tcggaaccga aaaagatttc aaagaactga ttaaagaagc acataaaaga aaaattcata
    12721 taattctgga tatggtaatt aatcacactt cgcaccagca tccctggttt attgattcat
    12781 cttcatcacg acaatccgct aaaaatgact ggtatctatg ggtcgatggc aaaaaaggaa
    12841 aatttccaaa caactggatg agcgcgtttg gtggtcatgc ctggcattgg tctattgaaa
    12901 gacaacagta ctacctacat ctttttctaa aagaacaacc ggatctaaac tggcgcaacc
    12961 cagatgtgca aaaagctgtt ttttcagata tagaattctg gcttaaatta ggcgttgacg
    13021 gttttcgctt ggatgttata aattacattg ttaaagataa agatttcaaa aacaatccat
    13081 actttcttca caagaccaag cctcgcaggc acgacttaca acatcatcaa catgaccgca
    13141 atcaacccga aacacatatt attttgaaaa aattacgtaa acttatgggt aattatgggg
    13201 aaaccatgct cgtaggagag gtttacccag atgaagatgt aatgaaacct gaaataccag
    13261 cctcttatct gggtaacgga aaagacgaat tgcatttggc atttgatttc tcaacaattt
    13321 ttgccaaatt ttccgcaaaa tgtttccgca aaatgttgac ccgttggtat catgccatac
    13381 cacaagacgg ctggccctgc cacgttttat ctaatcacga taagagcaga gctatatcgc
    13441 gcttagcgaa aggaaacaca gataaagcaa aattacttat gacccttctt ctaacacagt
    13501 atggaacacc ttttctgtat tacggagaag agatcggaat gacggatgtc cccatcaaac
    13561 gaaaggatat cgttgaccca ccgggaaagc aatactggcc cttttataag ggacgcgatg
    13621 gagttagaac acctatgcag tggaacagta aaaaaaatgc ggggttcagc aattccaaac
    13681 catggcttcc tgttagcaaa gacagtgtcc acatcaatgt aaaatatcaa tatgataaac
    13741 acgattcaat gttaaactat acaaaagcac ttatcaggct aagacaaacc cttactagcc
    13801 ttaatcgtgg ctcaattaat ttcgaatcat ctcattcatc tgtattagcc tatagcagaa
    13861 cacaccaatc agaatctgtc atgattttgc ttaatttcag tcgtttcaag aaaagctata
    13921 acctgccatc taatgattat catatagttt taaattcact aattacaaac acaacaatag
    13981 ctgtcagaaa caatatcgtt ctccaacctt atcaagcatt gattataaaa aaagagaatt
    14041 cctgattcac tgcagaaaaa agtaattatt tgaaataatt acttttttct gtatcattaa
    14101 tagaaataaa taaccttata tttattatat tatgaggata taacaatgtt actaaaaaga
    14161 ttactaatta tttttgtatt aattaattca ccattttcta tttccgcagc cgaccccaag
    14221 cctcaactaa tgtacaaatc aaattctgtt atttggggtt ttacatttat taatgcaaat
    14281 gagattatta tttccgaaaa atctggtaag ctcggtttat ttatggttaa ttcacgacaa
    14341 taccgtgaaa taaatcacaa tatcccgttt tctgttcatg gtcagggtgg tcttcttgat
    14401 gtagcatatg attcgagtat tatgacacta tttatcactt atgcaagaaa aacaaaaacc
    14461 gatctctaca caaccgccct agcctctggt aaatattcag aaaacactaa ctcaattaat
    14521 tttaaagaaa tatttgtctc aaaagccgaa tcacctaaag gtgttcattt tggatcccga
    14581 attgctcttg ataaaaaaag cagacaatta tatatcacaa tcggagacag gggcctaagg
    14641 aaaagggctc aagatctcgc atttcataac ggtaaaattc tccgattaac ctataatggt
    14701 gatgctgtat ctaccaaccc cttctacaat aaaaaaaatg cgttacctga aattttcagc
    14761 tacggacatc gcaacccgca aggaatatac ataagaaaag acggacatat atttatttca
    14821 gaatttggac ctaaaggtgg tgatgagata aatattattt tacccggaaa aaactacggc
    14881 tggcctcttg caacttacgg aaaagaatac tccggtgcaa ccatcgcccc tacacatcat
    14941 cctggaacag aacagcctgt tgtctggtat gtaccggcta tctcaccctc aggcatgacc
    15001 atatatgaac atgattattt tccacagtgg accgggcaaa tattcatggg agctttatcc
    15061 gcaagacact tacacagact caatataatt caaggtgaaa ataaccaaaa agttaaaatg
    15121 tactctgaac tgaacaaacg agttcgtcat gtttcataca acgacgtagg gcaaatattt
    15181 ttttcaacag atcatgggga actctatatt ataagcaaat aaaaaccgcc catccggata
    15241 aaatccgtcc ggatgtccta cactttttta aaatttttag tgacatataa ctaacctttt
    15301 gatattaatt tacggttagt taccggaagc actgcccgtc aggcaccata aatattcctg
    15361 tacgcaacta agcggtaacg gccctgataa cttataaatt caaacttgta actctattta
    15421 agcagttaca ataacaagga gaattaagat gacagggaca atgacagctc aatcatcaat
    15481 ttatctttca aaaaaactta cagcagccgc aaaaaaactt ggagagcggg agcttataac
    15541 tcttgttcgc aaagctgaat ctttattaga ccagcaaaaa tcattccgag acagtgctaa
    15601 aacaaatcct ctttctgtaa ccgtttcaga cagcaccgac ggaaacagct ttaggctttt
    15661 gataggaagc acacctaaag tttttcaacg ttatgagatg cgaaaaatgg taaaattatg
    15721 ccaaaaagcc gacaacagaa tcagaggatc gcaaaaactt aaaatttggc taagaaataa
    15781 tcgtccagat ttttttcttg atcataaaat tggaaacaac tgggatttgg ctttagctga
    15841 aatatatgat tatcttatca ataactatac agtacaatag ttcattaagc aaaatatttg
    15901 ttaagccaac ataaaacact atctacccgg catcgaaata aaccttgccg ggtaaataat
    15961 ttatttatta aagtaaaaac tatttataaa ttttcattca tacaattatc acaatttcca
    16021 caaacaattt tttcagatga aaaatagtca tggatataat ttttacggca acctttctct
    16081 tttacataat tcaacattcc aagcagcctg attctatcat atttcatttt atcatcaata
    16141 cgctttaaat caaacaacga gtcatctccg ttatgtttta acctcaatgt tccatttcct
    16201 ggctcacctt ctatatatcc aaatctttcc aataacgaca atcctgtcgc aagacgatga
    16261 tcagatcgat tcttaaaaac cagttgctct tgaatatcct gatagcttaa tgaattaagg
    16321 gcagcctgac tattttgaag caaagaaaga gtgcgtttca aaaacttttg atcaggattt
    16381 cgccattcta gaaactgtaa ttgaactgct aaatcagatt catcataata aagcctacat
    16441 tccgaagaat taccatcacg accagctctt cctatttcct gataatatga ttcaatagaa
    16501 tcaggtattt ccgcatgaat aattgttcta atatcttctt tgtcaacgcc cattccgaaa
    16561 gcgttagtcg cgatcattat ctgatcttta ctttgaataa atttattttg agcatcttgt
    16621 cttctatatt tatccatttt tccatggtaa acaataaaat tcatattttt attaatcaaa
    16681 taatcgcaac atttttcaat tgagcttatc aaactgaaat agacaattgc actacctttt
    16741 ctaaatttta aatattctgt gatcgcctca aatttatcaa gctcaccata aacctgataa
    16801 accgaaaggt ttaagttatc acggcaaata ccatcattat atacatcaac atcatcatga
    16861 caaacaccta tcttagataa aatatcatta cgcacttctg ttgttgctgt agcagtaaaa
    16921 gctgcggtca caggattatt gagtatggcc ctatactcaa atatctttgc gtaatcaggc
    16981 ctaaagtcat taccccattg acttacgcaa tgagcctcgt ctagaacaag cattgatatt
    17041 tttctaaatg aaaccgattt taaaaaagta ctatttctaa atcgttcagg tgatatatat
    17101 aatagtttat attttccctg ttttattcct ctataacggc tctcacgctg atcatacgaa
    17161 agtgacgaat taataaattc agcatcaata cccgtttttt tcaatcggtc aacttgatct
    17221 ttcattaagg ctatcaatgg agatattact atagtcaagc cgtcaaagat aagggcggga
    17281 atctgaaaac acattgattt ccccatgcca gtgggcataa ctgataaaac atttttccca
    17341 ttcatcaacg actcaattat cttcttttgc gaagccctaa attcattttt tttgaaaata
    17401 ttacgcaaaa tccattccgg acttttagtc tcatttatca ttaaacctaa acaccctatt
    17461 tctattaatt ctatactctt ggccattcaa accaacttgt taatatcata agatgatcat
    17521 tagttttttt cccatacaaa atcaaacaca attatagcgc taagttaaac aaccgattgc
    17581 atttgacatc tttaaaaatt tttttataat tctatttgat attgttacct ctaatatgtt
    17641 tattttaaat cctaattata aaaaaagtga tgacaaattt tccaagcaat gatacaattt
    17701 aactgtttat tatattacgg aatcttttaa aaacaactaa caaactgatt gtaaaataag
    17761 gttttttaaa agtacttcta caaaggagag atgtttatga aaaaggggat tcttattttg
    17821 ctcgttcttt tcagtacttc aatgctttcc gccttaaatc ttcaaaaatc agccggatta
    17881 actcttaatg ctacacgtta cagttgggag tatgaatatg gtgacaatac ttcccaggca
    17941 agttttcctt actctgttat cggaatcgaa gcatttttcg atatgaccta tttcagagta
    18001 ggccttagct ataacagtag catgggtgat attactttcg aaaccgaaac tgacgatagt
    18061 tccacttcac aagaccttga atatgaattt tcaacctgcg atgtgatgct tattggcaaa
    18121 tatcctatta aaaagggcaa atttgacatt tggccaggtc ttggaattaa ctactctatg
    18181 aatatgactt tcaatgatga agacggtaat gaaatagaag atacggatat ggacgacctg
    18241 tatattttag ctgttcttgg aatggattac catatcgatt ccaaactcta tattacaggt
    18301 agccttgatt ttggttataa catcaatcca aagatgtatg acaacagttc aaatgaagat
    18361 gactggagtg gatataagtc atctataaaa atcggtgcag gctactactt ttaataaatt
    18421 tcaaatacag ggaagaaata ttcttccctg taaatcttct gcaataatta tttattcact
    18481 aatcgctaaa tttattaaat attaattccg ctaactcttt tttttactaa cttcttgttt
    18541 ttttgtcgct taatttgtct aaaaaaagcc cattaataca tttgacaacc actgctattg
    18601 gaagcatctg gatatataaa attattccag gcaggtattt catgcgccta ttgactattt
    18661 tcctatatct aattccctct atggcttttg cctctcagga tacacattca gcaaatataa
    18721 cacatatgat gacaaaactt gtcttcgaaa tcggtgtaat catattcgca gctaaattgg
    18781 gaggcattac agcagaaaag ctaaaaatgc catcagtcct cggagagttg attgctggta
    18841 ttattattgg cccttatgct ctaggcatca ttgttttccc cggtttcccg gacggactct
    18901 ttccaatatc tacagcaact gcgctaccga tatcacccga actttatggc tttgccacta
    18961 ttgcatctat cattttactg ttcgctgctg gtcttgaaac tgattttacc cttttcttga
    19021 aatattcagt taccggactt ttcctgggaa ttggcggagt tgttgtttcg ttcgctctag
    19081 gggcattaac aacaactttt ttcatgaacg tctcttttat gtctccacaa gctctgtttt
    19141 taggtgttat gagtactgct acttctgttg gtataacagc ccgtattctt tctgaaagaa
    19201 gaaaaatgga ttcccccgaa ggtgtttcca ttatggcagg tgcggttatc gatgacatcc
    19261 ttggtataat tatcctggcc atagtgcttg gaatatcagc cgcggctaca catagctctg
    19321 gcaactctgt tgattggagc aatattgcat ttgttgccat caaagctatc ggtgtctggg
    19381 ttttctttac ttttcttgga atagtatttg catataaaat aagtcgcttt ttgaaaatgt
    19441 ttaaatcggt atccaccttt tctgtaatgt ctttgggcct cgcttttatt ttggctggta
    19501 ttttcgaaaa agccggcctt gcaatgattg ttggtgctta tgtaatggga ctatcacttt
    19561 cacgtacgga tctaagcttt gttattcatg acaaacttca cacaatacaa ctatttcttg
    19621 taccaatatt ttttactgtt atgggtatgc ttgtaaatcc agcaaccctc ttttctcgag
    19681 aagttctcct ttttggtacc gtttatacag ctggagcggt tgccgcgaaa ataattggtt
    19741 gcggcattcc atcgttaatt ttacgtttta acaaacttgg atcactccgc ataggtcttg
    19801 gtatggttcc acgtggagaa gttgccctga ttattgccgg tatcggatta tcttatggaa
    19861 tactagacaa taccatgttc ggcgtgggta tcatgatgac tcttttaaca acacttatcg
    19921 ctcccccgct attgaacttg tcattaaaga gtgataagtc gggcactaga aaacaagttg
    19981 aaaagtcaaa ccaaataacc acaactttcg cgacaccatc tgcaagttta actaaaataa
    20041 ttgaagaccg tatattagaa aattttcacg aagaaggttt ttttattaac cttatcgtta
    20101 ctaatgttaa taactatcat cttaagaagg acgcaatcaa cattaacctc actgtaagcc
    20161 gtttcgttat tgagtttaaa acagacgaag ccgatttgcc atttgttaaa aatgtaatct
    20221 atgaaacatt gcttaacttt aaaaccgcaa ttacagaagt tcagagtatt gtagattctc
    20281 ttgatatgcg aaaagaaatt gttggcaaag gccgcacaaa taagtcagaa gtacgcaaac
    20341 accttaaaca tagcaatatc attattggac ttgaatcaaa cacaaaagaa ggtgttataa
    20401 aagagcttgt ttcagttttt aaagatttta aagagatcgg tgatcctcgg gatattattt
    20461 ccgctctttt ggaacgtgaa aaagtcatga gtaccgggct tgctgatggc attgccatac
    20521 ctcatgcaaa aacagagcag gttaaatcca tacagcttgt tattggtctt gcaccagatg
    20581 gtgtagattt tgaatcactt gacggtaagc catcaaaaat attttttcta atcttgtcac
    20641 ctttaaatga atcatcctct catcttcaga tgatgtcaaa tattacttct atattttatg
    20701 aaaaagcaaa ccgaaaagaa cttctatcat gtagtgatac tcaaagcatc atggatttct
    20761 tttcaaaata ggcgtttgtt ctattaattt attgaaatta acacatttct attcacttta
    20821 ttaaatatgg cagtataatt taaaacggat atggttagga atctaaagtt attcatatat
    20881 tttataatga aaagtcaaag aagatatcag ataaagaagt aaaataaaaa gcagtttatg
    20941 ccgaaaatat tgataaccct atttacagaa ctgcatctgt ttcgcgttac caatgagagg
    21001 ccatgaatac gactgagaaa atagaaattt acgactatga ttcagataca cttataagcg
    21061 agtttgatga aaacagcagc atagagctat atagtgaatc tggtaaacat attaattcta
    21121 acgaattaaa tcatgacaca tttacagtta gaacctccga accaatacaa aatgagacct
    21181 tagacaatga tcattatgta aaacaaaatt tatcagaacc atctgctatc gaagacgaaa
    21241 tttctatcaa taaaaataaa actacatcca gaacagaagc aaccacagta aacagcaata
    21301 ttaaaccaat atctgcccca tcccatgata aagaaactcg tattcttcct ttatcccgtt
    21361 taatgatccg agccgacaaa attgcgtacg caattattaa ccacaaagag cttgatgcct
    21421 ctttttttat cgcgccagat gagactccat taacacaaga tgaaattttt gaaatagtaa
    21481 atcaggcgga aaaacttgaa tttattgaat gtgaaggtat cggaaataac aacgatgagt
    21541 gcttttcaaa catctacacc gaaaatatca gccaagaagt caaaaatgcc aataaatccg
    21601 aatctaattc tttaaaaaat gtaaaaaaac aaaatttata ttactcttca cattttattg
    21661 aaaaacccaa tttagacgca ctacctaaac ccggtttatc cgaaattgag cgaacaagca
    21721 tagaagaaga tattttcacc gaacattccc ttgttatcga agaaagcata gaggatatca
    21781 aacaaaaact tttagaatat tggtacatgc aatgtgagca atgtgaagat atttctgaca
    21841 caattgtaat tctagaaaac gatagtgatg tagaaagatt cattaataat tttcccgata
    21901 tagcgaaaaa ggaaaaactt aaaactcttc ttgaatattt tggtaatctt ttcgaaaaac
    21961 ttcctgaaga tacccttaat agctttgcaa agtcagagta ttttgatatg tacattgatt
    22021 ttatgaatca acttcaaaaa aactaaggaa gcaaagcact taacaaatgg aaaagaatga
    22081 acaaaaatta aaggacatta gcgatattat cacagagtta gacaacacat tatctaacga
    22141 tacaattttt cttgatcttg aagaagatag taagtctggc aatgaacaat atatttctcg
    22201 aaaaaacctg ttaaatgctt ccgataaaat tatacttgat attgcaaaag aaatatctgg
    22261 agctaattcc ctaagcgaac tttatgaaat tctaattttt accgcaattg ggcaattagg
    22321 tatatcctcc gcttcaatta ttcatccttc aactgtttca acagaacgct ggatccttgc
    22381 agaacatcac ggaatgaggc tacgcaatcg caacctcaca ttcaaattga acgatactat
    22441 tttatcaaaa gcaattaata aaaaagagat aatctatcta gatgattatc aatctgatat
    22501 aaatgctcag gaagaatatc agaaattttt ttcgattgga gcttccctta tcgctccggc
    22561 aattattaat gaaaacctta tgttcgcctt agttgttggc tcaaagctca aacaagaaga
    22621 attttccgac aacgatatag tgtttttaaa tgctttattg gatataactt ccgtaatttt
    22681 tagtaaaatt tcacaaatag ataaattaaa aaatgagctt aaagatcttg caacatttca
    22741 aaaaagattg gatatgattg ataattacga agcagatgtt cggaagacat caactcatat
    22801 tgaaattgat aatattatac aaaaagaact ttcatcattg caaataaaca gctacgccct
    22861 tttttttcgt aacgattcaa caaatatgtt cgaaattttg ttcaccgaaa aagaagatct
    22921 tttgcatctg aaacgcaagg gatacgaaat taccagctca aacgctttta tatcttattg
    22981 tttctcaaac gatacttata ccgaaataga aaatcctgta acatcccaga ttttgaaaac
    23041 agtttttaac gacgattttc ttcttagaat taatgttttt ggggtttatc cttatttaat
    23101 caaaaaacag ttattaggtt ttcttcttct gttaagagtc aatctggatg tatacaaaac
    23161 tcatataaat caagttaaaa gatttagccg gtttatcttt tctcacctgc aaacaagtaa
    23221 atatttaatc tattcaaaat ctttcagcga tgttttacaa cctcttttaa cacgaatgcg
    23281 ggaattacaa atatcatgtc aaaactcacg tattccacta actttgataa ttatcaaaat
    23341 tggcggttac tcaaactaca tacaatctgt aacgcttcaa caaatagctc tatgcaatac
    23401 tcaaatagaa tctatttttg acgaaaaact gcaagatgga gattatgctt tcagattcga
    23461 catagacagg tatatagtag ctcttcccgg ccggaaaaaa cgggctggtc ttggttattc
    23521 aacaattctt aaaagactta ttgatgagat agaagaagaa tttactgtaa ttacagctgt
    23581 tcacgaaatt cctaaagacg gctcggcttt tgaagaattc ttaaaagagc tatcatgatt
    23641 ctaccgcaaa acagtcattg gattaataac cgatccacct ttacgcactt caaaatgaag
    23701 atgtggcccc gttgaccgtc cactgttacc tgacagagca ataatctgtc cagcagtaac
    23761 cgtatctcca attttcacct taaacttact taaatgccca taatatgtct cataaccatt
    23821 tgtgtgtttt ataattacca atttaccata gtttccattg cttccacaaa aagtaatttt
    23881 tcccgatttt gacgccttta caggcgtacc gagtgagcac ttaaaatcaa ctccactatg
    23941 aaacgacgga atacgcaaaa aaggatcaat cctgtttcca taagctgaag ttattctggt
    24001 tttatctaat ggtttacgaa accctggact tagaaatgcc gaacgttctt ctcgcgacaa
    24061 gtttccgcaa ggaataaaaa taaactcttt atccaattta ttaatcttgt ttacgtattt
    24121 tatataacgg ggattaacct tatactttaa gcctatatcg ttaattgtct catttttgtt
    24181 taccttatgc actatccctc tcatattagg gataaatatt gttgagccaa cagatatatc
    24241 cgatggattt tctaactgat ttactgtcat cagagtatca atattcaatg tggttctagc
    24301 tataatgttc cagaaattat catctttttt tactctatat ttataaaact taagttcagg
    24361 tatctgtttt tcatcccggt tgctttttac agtagcaatt gtagttctta tctcagcacg
    24421 caggtttttt atctcctcac tgcttcgtga caagtttttt aatccgggtt ctaaagctaa
    24481 tccggatttt attattaata gaacagagat aaatagtatt gtttttttca tagaatgtga
    24541 cataacctct aattttttta tcggaagaaa acttcaataa tttgacaatt ttttttcaga
    24601 aaccaaaaat acctttactg atactatctt ttacaaatag tatcagtaaa gttcttgatt
    24661 ttattactgg taatctctaa taattaattt tcaacaagga acttgccctt gtaggttgtt
    24721 cttatcagaa caataagctt ttagcataag ataaaagccc catgggtaat acgaaaaatt
    24781 cctttaataa agccataaat atcgctggct tcaaagcgat atttattagc gttttttcga
    24841 gtgacaacga gaaaaattgt ctttaatagg ccttaaacgg catagccgtt ccagaaatgg
    24901 taatttttcg aagttcccta ctgttagttt tatataatct taacctattc gtatttatta
    24961 caggagatac tatggcactt gcaaaaagcg gtggtgatac tgccaacaat accattggcg
    25021 agagttcata ctttacaggt aaatttcata ttaatggctc acttcgtatt gatggttgtt
    25081 ttgaaggcaa atatctaaaa gctgatcaac tatatattgg accaaaagga cgtttaaaaa
    25141 caaatatcga agctgtttca gttattgtgg aaggcttgat aattggaaat atcaatgctt
    25201 ccagtagggt tttattaatg ccaacagcca aggttcttgg agatatcaga actcccgaac
    25261 taatcattca aaatggtgtt attctagaag gaagatgcac tattgccaat gaccttaagt
    25321 cttcggcaag agaacttatt gataaagagt acctgaaaaa caggttcgta ccaaatgagt
    25381 ttgtcgaatc cagaaaacgt gcatagattt atagcaatta ccgttggaga cccttccggt
    25441 attggtccgg aagtgacttt acgtgccctt gaccagatta accaaacaga ttttactact
    25501 gttgtaatag gcaattatga attgctaaaa aatctttctg tatcacttgg aatcaaaaaa
    25561 ccacttaaaa cagtagacaa tgattttttt catgcccctc ctgataattt aaaagatatt
    25621 tttatatacg atattgattc acaccttcca atccctactc ctaggtttcc ctccgtcgaa
    25681 accggaaaag aatcccttgc ctatattgat aaagctattg aattatggaa gaaatcttat
    25741 atttcaggaa tagtcacagg ccccgttagt aaaaatctta tagaaaaaac tggtatacgt
    25801 ttttcgggtc atacagaata ttttgctcaa atgataggtg aagacactcc atatatgatc
    25861 atgtattctg ataaatactc ggttatactc gttacgactc atttacccat taaagatcta
    25921 gcaaatcaca ttacaaaaga taaaattatc aaaactgcaa tcgaagcaca aagaagtatg
    25981 actcttataa gaaacaagaa acccaagatt gccatatgtg gtcttgatcc tcactgtgga
    26041 gataatggtg caataggttc attcgatact agtataactt cccccctggt tcaggaactg
    26101 caacagaatg gaatggatat ctcgggtcct ttcgctgcag acaccctgtt tttaccgtct
    26161 gaatgggaaa aatatgacat cgtaattgcg cattatcatg atcaaggatt gattcccttt
    26221 aaaatgatgg cttttgatac aggagtaaat gtaacaacag gattgtcgct aatacgtaca
    26281 tcagtcgatc atggaacagc ttttaatatt gcaggccaag gaattgcctc attttcttca
    26341 atgattcagg caataaaact ttgtaaatct ctaattagga ataaatctcg ctaaatatcc
    26401 ttaagctcgt gattaattgt tctgattgtt ctaaaaacca gatctttacg gcttggagca
    26461 aaaagttcaa taaactttac acttttcttg tcatcacctt ctagaacggt aatcataaat
    26521 cgtatatagg cctgtttctc ttccatcacg acattaccgt ctttattacg ggaatagatc
    26581 tcactcttgt tattcatggt taaaattatt acttcggcac ggtaatcacc gctgatttta
    26641 ataaggcttt tttctattaa taaggtatct ttatcggaaa aattgggact ttcactgcga
    26701 atttgcaaat catttgccaa aacaaccgac acaaagctcg taaacataaa aacagttaca
    26761 acacaaaact taattctatt tttcatttta tcttttaccg ataattataa tatgtgatta
    26821 ctattatgct aaaatggact tgttatggaa acaaacattt ttttgaaaac aatacctttt
    26881 tttatcttca tatggggatt agcagctgca tttttgatat ttagaccacg cattccagta
    26941 ttatggaaaa ttgctgcttt tctactttat ttattctata tttgggtgtt taagtcagat
    27001 atagaacaat tccttattat ggttggccaa aactggtata ttacgactat actgtttcta
    27061 aaagaatctc tttaccttac attttacctt cttattatct tttggccaat ttctttattc
    27121 ataatttttt acaaatccga tgattttgga gctgaaaggt tactaaaatt catgattttc
    27181 tttacgatta ttttttggat tattgttgct gtctattctt ataacaagag gggaatcgac
    27241 aacctgcttt ataacaagct aacagaacta ttcccgtttt taaaatgaaa agatttattc
    27301 ttttgtttct ttttattttt tcccaaattg aagcagcaga aaacagtaca ttactatatt
    27361 atcagggacg agagcttgcc ttgaatggag atatagaagc tgccgcagaa aaattcaagc
    27421 aatcaataaa acttagcccc tattatgtac ttccacatta tggtttaggg cgctgttatt
    27481 taaatatgga gggtaaaaga aaagaggcta ttagggagtt aagaatctcc ataaaactcg
    27541 ataagaaatt ttcaccagcc tatttttacc ttggtctcgc ttatatgttt gaaaagcaat
    27601 acgcctatgc acttaaccag tttaaaaacg ctctattata cgataacaca agaaccgaag
    27661 ccctttacaa tatcggagca gtttacgatc ttatgggaca tccgaacaaa tcattactct
    27721 attatgatca atatttgcgt gcaataaaaa cagattccga ctatagagaa taaacaaaag
    27781 agaccctttc gggtctcttt ttataacata acttactttt tttaattatt cagtaaccac
    27841 ttcttcttct tcaacaacgg taattccaga agatggagga ataacgtcaa caaatatcga
    27901 aagaatcacg atctcggatt tatttccaac attatctatc gcttgtgctt cgataaaatg
    27961 atcgccatta gttggcaatg taaaagttcc atcatacttt ctaaatgcac ctgtaccatc
    28021 tattcgataa agaatttctc gaacacccga ttcggtatca tttgcattaa tagtataaat
    28081 atagtcagta ccagcaacct ttttaccttt tctttctata aatggtctat cagctgtaat
    28141 atcaacagta ggagctgttt tatcgacata aaagcttaaa gaactttttg tcattgatgt
    28201 aggtgtacca ctctcatcag gaagataaac tcggaattct tcggaaatat tgctaacatt
    28261 atcttcagag cgaacacgga aatcaagatc accctcggta gtaagattaa aacttgttga
    28321 atatcctgta aaatcagtac cgttaacaga atattgaaca ttgtttacac cagacattgc
    28381 atcacgagta gaaatcgaaa tactgtttgt ttctgaaata taatatttac cacctatttc
    28441 ataaacaggt ttttccaaag ttactattga ttcaggtgct gtattatcaa tataaattgt
    28501 aaaaaccttc ggagtctcta cgttacctat actgtcaact ccccaatact tgatattatg
    28561 tcgtccctca tcaggaattg aaaatggaga aagataatcg ttttcaccag catcattaat
    28621 tgtatagata agtttatcag caagaaagtt gtcagaagta tgaagcttaa actgtactct
    28681 tgaattcgcg taggtattta ctccatcaaa ataagtctta gcaacagtag tagttgttgt
    28741 tgccgtagat gtagatgggt aagtatccgt cattgttggt tctgttacgg tatcaatacc
    28801 gctaaccggt gcgacatcat ccgcggaatc atccatggaa tcatcaacag catccagacc
    28861 tatatcagct gttgggctgt cggtagttac ctcttctagt gaagaataat cactggtaac
    28921 cgcctcagcc tcggcttcgg gttcttcctg agcaagaagt gttcccaaag atagggcggt
    28981 aatcagcaat aatagcgcta attttttata catgtaatcc tccgtttata aagtgacctt
    29041 gtaatagtga ataagtgctc tgtttaaaga cagataatgt atagtgaaga aaatatttta
    29101 actgttgtca atataaattt gcaggaacca tctaaaaagg gatattaatg cagctacaga
    29161 ttatgcttat tgattataag tatagcctgc acggactttt tattctataa atatatttac
    29221 tttccactca taagacaatt attctggcat aattaaaata ccataattga taattgttct
    29281 aataataacc ggtatcccct tttttttgtc aatatgcggt ttaagcgggt caattacaac
    29341 agccttgcta actccccgat atttataata cagcttgtaa taacatgatt catctgcaat
    29401 tctatcatca gaatcaatat ttaacagatg ttctctatcc attatggtta tgaacttttt
    29461 atatctttca ggatgaatag atcttttccg tgtatttgat cgaaaatttt ctatttgaac
    29521 ataagccgta tggccgctac ggaataccgt aacagaccat tccagagtag aaactggctt
    29581 tgagtaataa ctgagtctaa caattgtctc gaattcatct gcttgaattt ttgtactgag
    29641 caatattaac agcaataaaa ccgaaattct taattgcttc aaatttatat cctcgaaaac
    29701 aaacctattc taaagtctgt tactctaatc gacacaatat tctatgtatt ttatgtaaaa
    29761 cagacgttta ttgacagaaa tcgtgaaaat tttgggaata ataaaatgca ggattaatta
    29821 caatatccgg tcaaaagaac actgccgccg tataaactat gcagcagata atcaataaac
    29881 cggatattaa aaataggaaa ctctgttata aacaacagat gaaaactatt tctttttaac
    29941 tgtttttttt gcagttttct taactgtagc tttttttgct acaggctttt ttgcggcttt
    30001 ttttactgct tttttagtta cagccttttt cacagctttt ttcgctgatt ttttaactgc
    30061 aggtttcttt gcagttttct taactgtagc tttttttgca gctttcttag cagctttctt
    30121 agcaactttc tttacagccg gttttgaagc acttttttta gctgcttttt ttgtagccgg
    30181 tttttttaat tccttttttt ctgtatttgc ggaagcagat acagctgatt ttgtctgagt
    30241 tgatttctta tcagatttaa tcattttgga agcagcagcc acacgctcac tcatccattt
    30301 tgaaattgta gttaaaactt ttgatctgtc ttttccgttt tcgttcataa tctcatggta
    30361 aaggtcttca aagaccttca tctccttatc tgttgacata attttatcaa aaagaagctt
    30421 gcttcctttt atatccgcta ttcgatcatc tccaccgtgg agaattaaag ttggcagacg
    30481 aatttccatg ccccgaccca aacagttttc agcattttta ttatattcac aataccactt
    30541 caaagttata cggtcatgaa caagctcatc agccttgtat agttctgcaa cctcttcatc
    30601 atgtgacaga aaagatacat caagtttatt agaccgttgc atccccggta caattttaga
    30661 taaaaacatg ctcacattat gtaaaaaagg agaaacttca attttaggta taaatgcacc
    30721 cgcactgatt atcagacctg ataaatcttc ctgatattga agcgcataac ggcacgctat
    30781 tgttcctcca aggctatgac ctaacatgta tatcggcaat ccaccgtgtt tttccttaat
    30841 caattcaata aaagatttta aatcgtgtac ataatcaaga aaagagtcaa catgccctct
    30901 tttcccacca gaacgaccgt gcccccttaa atccattccg tatatcgtta taccttttcc
    30961 attcaactgt tcaacaagat ggttataacg cccacaatgc tcaccaagac catgaacaat
    31021 tataagcaaa gctttttctt taggcgaaac ccaagactga tgatataatt caatatcatt
    31081 tttacccgtt attcgtccct cttcatgact atagtttaac attacatgac tccatcgaca
    31141 aaaccactat ttactttaac cggttaaaaa ccatcaataa accagtttca gttgatgtat
    31201 aacatttata tatattatgt gcaagaaaaa aaccaccacc tgctacgaaa aattgatata
    31261 tttgcaaaac cacattgaaa acacctgatt ataagctttt atttaaatta atccctattt
    31321 ccaaacagac ttatacaaat agtagcaaat acacagatta cattttaata ttatcaatcc
    31381 gagcaggcga attacggaga gcaaaccacg cttaaatcta tttcaattta ttcttttttt
    31441 cggcaagttt taacgcaatc tggtttaaca atacttctat ctgaaacggc ttttgtaaaa
    31501 aaagatctat ctgtttatta tagctcttga tatcttcgat catccatccg gtcaataaaa
    31561 cagtcaacgt tgtctctcgc aactctctta tttcagtcaa taactctgta ccggtcatac
    31621 cctttaagcc ataatcagaa attacaatat caaaatgacc atttttaaac tcattcaatc
    31681 cagattcggc atcttcatgc acaataacac ggttacccat aaagtcaaat aattcatata
    31741 gaatttctgt taaagcaaat tcatcttcta ctataagtat gtcacaatcc cttatcttct
    31801 gcttatcact gttgctggca gaatcttcac ctgttacaat catttttgcc ggtattaccg
    31861 cgctcaaaga gataatatcc gctgattcct cttcaattat tcgtacattt atcttgtcag
    31921 cgatgcgtct aagttcaatt tcaggtaaaa aatcttcccc ggtctctaat gggtcattat
    31981 caaccaaacc acgttttacc gaaacagtga atataatatc atcatccttt ttcaattcaa
    32041 tatcaacggc tccactctga ccaatatatg atgatattcg aaaaatcata gaaacaacta
    32101 tctcgcgaag aatttttaaa ttccctgcta cacgtgcttt tgtgtaatac agtttactga
    32161 tagaaatcga acgacctttc tctttacgct cgactttaaa atgtattgat gcaaattcta
    32221 tagcatccct tataagctca attatttcaa cgctaccctg gttatattca acagaatccg
    32281 aggtgataaa ctcctgtatc ctgcgtattt gagtggctgc atctacggcg gattcgttta
    32341 tagactgtaa tccatcaagt atatttggtt tatctgtaag ctgagacagc atttgtgatc
    32401 ggttaatgat aacatttata agattattga acgaatgcaa cagtcctttt gtcatatcac
    32461 taaatatttg aaccctattc atcttgctta actttgactc aagcatcttc aacctagttg
    32521 tatcttcaaa aacaatttcg atatcatgtc cgtttatgtc agaatcaatt ttgtataatg
    32581 ccgcattgca cgagttttcc actcctgttt tagtgttcac tttcaagtca ttaatacgta
    32641 caaaagattt tgaaccattg catatatcta caaccgcggc tttaagcttt tcagaacaat
    32701 atgacgtaaa aaatgataaa aagcaatctg ggttctcaag agcaaaatca cgattagtac
    32761 caaaataatt ttccgcggaa gagcttatcc gtaccaaacg agcagaagta tcaacagtaa
    32821 caaccggaca tggagaatag tcataaagcg aagcatgttc gttgggattt tgcatcgaac
    32881 gatcaaatga cacaactctg tcttcaaccg gcctgattat taatgctata cttctttcat
    32941 tttcaataag catgggataa agatataatt cggcaggaat tttcttatta ttgtgacctt
    33001 ttaatacaac aaaaaaccga gtatcaaggc cggaaagagt cttggaaaaa ttaagagcaa
    33061 tttttttgcg gtactcatta aacaactcat gagagtcatc aagagttaat tcagaagact
    33121 cacttgaacc ggttaaaacg gtaaatgatt catttctaaa tacgatctct cccgaagaat
    33181 ttataataca gcacggaaca gaaagagaat gcagaacaga aaaatccacc cgtttctcct
    33241 aacaaacaat cctttatcta aatcactaat caaacgcata atacattatt acacgatatt
    33301 taagtaatat agttataata atgtcagttt tgatagtggg gtaaattaat caattattta
    33361 ttaggattta tacaatttaa tttataagcc cttctaccgc aatgttcttt tattaattat
    33421 tgttttacta tttttaagaa tttctacctc ctgatcttct gtggcaaaga ccttgccatt
    33481 cgcatcaact tgttcaaacc tcaaagtata tttcccaggt tccaaaaaaa ttttcgatat
    33541 ttgcaaattt gacggaagtg ttctccaaca cctaagatcc ggttttattt gcgaagcaag
    33601 tgccgatcca actccggcac ccgtgagcag gccaattaat ggagccgcat cttttaacgg
    33661 tgtctgttca gctatctttt ctgcggcaat agaggatgca acagtcgcca ctgctttcag
    33721 ggcaattccc attgccactt tttctcgagt atcttcatac cttttttcgg cacttaacct
    33781 agctgttttc tcaatatctt caagagtttg tgttttgccc aatacttttc catctatggt
    33841 tctcacattc aaatacgctg ttttgttgcc cggactgata taatcaggta ttggatgttc
    33901 aatactgcct aaagcaacca taatagcggc aagagtaaca ccctgttcaa gagtcatacc
    33961 gttaacagac actataatcg ccgttttcat attagaatca gcaagtaatg atccacgaga
    34021 ttttttatat gcacctcggc ctgtctgatg tattataaca agctctccct tgtctttgta
    34081 agaaggcact gctttttgtt taaatttttg cttccacttg gccaaatctt ccttgtcatt
    34141 catgatatga gccaatctca aaagatcatc atgtactggc tcataatccg atctcatctc
    34201 gtaaatacgc ttatattcta tgtaagcaaa ctcaagcgct ccttcgtcgt gatcttcatt
    34261 agcaattatc tcataacata ttgctgtcag atatctggcc ataatattct gtttataaag
    34321 actttcgcca tttttacgca actcatcaag gcgtctacta acacggttaa actctacgcg
    34381 agcactatca taattcttaa gcattaaaaa atttattccc gcgtacatat gtatcagtac
    34441 tcgttcaaat tcttcgccgt gatatcttcc ttgccgttca tttaataata ccgaggcagc
    34501 ctgctttctt atgctaattt tttgatcctg tgcaaaactg gcggccttaa gcataaactc
    34561 attacttttt ttgtaatcac ccgcggtatg atacataaga ccagcttcaa tcatatacaa
    34621 tagccgctcg ttgctctccg cttttgcaag agtagagagc atatctgcac cggccaggta
    34681 atcaccacca taaaaccttg attcggcatc gttaaccttc gatgagtatg atgagctaca
    34741 gccataaaga agcagatatg ccagaacagc aattattgtt atacatttat ttttcacgat
    34801 agattaaaac tctttgcctt tatcttttga ttttgattta agaaatcttg cgttttcttg
    34861 ccaatgtatt tctccggttt tgatattata caatcgcatt gttacaacca aaagttgtat
    34921 ctcacgacca tctcgtgtag aaaccgcctc tctaatatca ccagatagat aaaaatttgg
    34981 agatttcaaa aatccggttg gaacagcagt agactgatcg tataaaccgg tcatcccttt
    35041 ctccatctct tttatggaat cctcggccaa agattcatca agaaatttta ttcttctttt
    35101 tattaattct gtagtcatct catctgtgac aacaccggta tcaatatgct ctgctgttcg
    35161 attacggaat ttcttgacct gtaaataagc tggtccattc caatcctctt ttagaaaaac
    35221 atataacgat tcaaccattt tctcaacaac caacttgact tctagagcac cccattcacg
    35281 agatccatga tcttctgtta cttctcgctc atatacaact tctggtgcgc taacgcagct
    35341 gcttatacta attgtcaata ctgttaagca tattaaatat tttttcatca taaaactcct
    35401 tgcaaaatga taaaacagcc tatcattatt ttttatttag tcaacaagta ttaaacacca
    35461 aaacagacta ctatcagccc tttttttata cttttttgtt taattaagct taataagtca
    35521 tttttttcat cgaaaagtta ctatttatcg tctttaatgt tttagattac aataaagata
    35581 ttacgaaaat ttgagctttt gaaaccgata atcaataacc agtaattttt tcattgtcaa
    35641 ttgccttgtc ataattatta tctaatcatc tttatggact actaataagg ttaaacctgt
    35701 gtttttagga taatttatca ttatggatat tgcttcaaaa cttggtatat acaaaaaaaa
    35761 atcacagccg attacagaaa atgcgacaaa aaaatcgatt atatttcccg atgaatctat
    35821 tgcggacaaa ttgtctatta atggtggtac agtctggaga tttgaaaacc ggctctcact
    35881 ttttgatgtt tacagtccgg taaatatttc accaatcaga gaattggacg cttttttgga
    35941 aataaacggc ttttccaatg atttttctct gtccgatctt ctcttttttg atctggaaac
    36001 aacatcactc tccattgcat caggaaacta tccctttgtg tgcggcatcg gatatacaga
    36061 tggagacgat tttgtaaccg aacagctttt tatggaaagt tattctgacg aaaaagcaat
    36121 actcacatac cttttagact tttttaatcg ttccaaagct gttgttactt tcaatggcaa
    36181 cagttttgat ttaccattga taaaaaacag atatatgcta aatagaattt atggttttcc
    36241 ggtcaacatt ccttcaatcg acctaatagt ctcttcaaga agaattttca aatcactata
    36301 cgaaagctgc tcacttcaaa ctcttgagaa aaacattctg ggatttgaac ggattgatga
    36361 tattccaggc tggatgatac cggaaatata ttatacctat caaaaggatg gagattcatc
    36421 ccgactgcct ggggttatag aacataacat gtatgatatt ttttccatgt atattctttt
    36481 acaaattctc tcttcaatat tttccgatat taaaaaagga aacttccgca atttcgcaac
    36541 ttcttcttta tttacaatcg cccgttgcct atttgtaaac aatacagaac ttttcttaca
    36601 attagccgaa taccttggaa cagatctttt tggagatgat actgtatttg aaaaattttc
    36661 tattgtgtta aagcgcaata aagagtggta caaggcggta gattactgga ataaatcagg
    36721 ctcctttttt tcactaatag aactggcgaa atattacgag cataaagaaa aaaaatacat
    36781 tcaagctctg aatgtatcta ataaagctaa tgagattttg tcaaaaaagg aaaccaacaa
    36841 agacgaaaac cccgataaag ttaaaaaaag aagagatctt ctgcaagttc ggataaacag
    36901 actcaaaaaa aagatctcag catcaaataa tgggacaaac cccagcctct gattcctcaa
    36961 tttcatattc aataacaatc atttcacctt ccactccggg aaacatctcc gcattctgcc
    37021 tttcgacctg aaaactgata ttacccattt gaaaagtaaa gctttctgcc ggtattgctg
    37081 ccatgaagta ctctcctgca tcgaattacc cgacaaatat tctataccaa atgtcagata
    37141 taatcataac ataataatta atgaaaatag caagctaaaa tcacttatca ttcaaaaaac
    37201 aattgctttt ctgtcaaatc cttataacta ttctaaacaa ggtatatcat aaattattta
    37261 cttgtcaaaa aatttagtga aaattcattt taattggaac tctgttgttc aacaagctga
    37321 atacaacaaa tttggcttta gttaataaac tataagcact ttgcagggcg ttatcatgtc
    37381 ctaactaata ttaagaatta ccggaaggaa ttatggaaaa aagagaaatt aaactaccac
    37441 ctgttatcga aggccttgaa ggaatggata ccgagtcaat acgtagaggt atcggaacaa
    37501 atctggttta ttcacttgct aaagacagat tttcagcaac agatcgggat atttacgaaa
    37561 gtttagcgct caccacaaga gatcgcctta tcgaaaggtg gataaaaaca cagcagacct
    37621 actacgataa ggatgtcaag cgtgtatact acctctcaat ggaatttttg gtagggcgga
    37681 cactaggtaa cagcctgata aatcttgata tgatgaatca aacatcagat gctgttaaac
    37741 agatcggata caacctcgaa gagctacgtg aaattgaatt tgatgccggt cttggcaacg
    37801 gagggctggg aagattagca gcctgttttc tggattctat ggcaacactg gcttatcctg
    37861 catatggata cggaattcgt tatgaattcg gaatattcaa gcaaaagatc agggatggtt
    37921 accaggtaga ataccccgat aactggttac gttacggaaa cccttgggaa cttggaaggc
    37981 cggaatatct ttttccgatc agtttttatg gaaatgtcga atatgttcct gatacagaga
    38041 acactgctgt aaaaaaatgg gttaattgcc aaaaggtcta tgctatggcc tacgatactc
    38101 ctatcccagg atataaaaac aataccgtta atacaatgcg tctttgggca gcaaaagccg
    38161 accgagaatt tgatttttta cactttaacg aaggtgacta taaccaggct gttgcaaata
    38221 aagccaattc tgaaaatata acaaaagtac tctatcccaa tgataataca atgcagggta
    38281 aagagcttcg tttaaaacaa gagtacctac ttgttgccgc ctctcttcaa gatattatcc
    38341 gaagatataa aaaatatcac gactcatttt cacaatttca cgaaaaaaca gctatccagt
    38401 taaacgatac acacccttct ctcgcgatac ccgaacttat gcgccttctt gtcgatgaag
    38461 aacgtcttga ctggaattca gcatgggaca ttaccgttaa aacttttggc tataccaatc
    38521 acaccgttct tcccgaggct ctcgaaaaat ggcctgtaga tcttatcaaa agagttcttc
    38581 cccgtcatct tgaaattata tataccataa acatgaagtt tatggaactt attaaggaaa
    38641 aatataaaga tgacgcaaaa cgtatggccg ccatgtccat agttgaagaa ggagaataca
    38701 aaaacatacg catggctaac ctttcaattg tagggtcaca ttctgtaaac ggagttgcag
    38761 cgttgcatac agaaataatt aagaacagct gttttaaata cttttacgaa ctttggcctg
    38821 aaagatttaa taataaaaca aacggcataa cacaaagaag atggttaaag ctatgtaatc
    38881 caaaacttgc tgagcttatt tcctcacgaa taggcgaaaa ttgggtaaca aatctttttg
    38941 aacttaaaaa attggaagat cacatcaacg attctgcttt tagggaatca tttacctcta
    39001 ttaaaaagca aaacaaaatt gatttcgcta gttatattga agagcacaca ggaattaaag
    39061 ttaaccctga ttcaattttc gactgtcagg ttaaaagaat tcatgaatac aaaagacagc
    39121 tacttgccct tctttatgct attaccgcgt atcacagaat aaaagatgat ccttcacgag
    39181 actttgtgcc tcgcacaatg atattctccg gcaaggctgc tcccggttat catatggcca
    39241 agctgataat aaaacttgcc acatctattg gtgatgtagt aaataatgat ccagacgtaa
    39301 atggaaggct atccgtagta tttgtagaaa attactcggt gtcaattgcc gagcgcctta
    39361 tacctgcagc tgatctctct gaacagattt ctactgcagg gtttgaagcc tccggcacag
    39421 ggaacatgaa gtttgcttta aacggagctt tgactattgg aacccttgat ggagctaatg
    39481 ttgaaattaa agaagaggta ggggacgata atatctttat atttggactt actgcagatg
    39541 aggtagaatc gacaaaatac tccgggtaca acgcaagaga atactatcat aaaaacggag
    39601 aattaagacg agccgtcgat gcgatatcta atggctattt ttgtccatcc atgcctgatc
    39661 tgtttaaacc aataaccgat gcacttctta atcacggaga tccctatatg cttcttgcag
    39721 actatgaaac atacatcaac atgcaggaaa aagtcagcac tgcttttacc gacaaggaaa
    39781 agtggactac aatgtcaatt tacaatgttg ctcgaatggg taaattttca actgaccgaa
    39841 caatacgtga atatgcagaa gaaatatgga aaattgatcc tgtctcagtt aaaattgatt
    39901 aaacagctat agacgctcaa agaatcattt aaacgttcct ttatactgat ataagggaac
    39961 gttttgttat ttaatttatc acaatttcta ttattaaaat cgcgtatttc aacaaaactc
    40021 tttatttgag acggtatgat tgtttgaatt ttcgttaaca ttacctataa caacggaaat
    40081 ccagattttt acaaatttct gacgcatcag aggataatca tgaagcagaa cgagattagt
    40141 catttaaaac aatacatatt ttttttatta ttatccggat taggaggtgg tttttttggt
    40201 ctagttattc ctaaatatag cgatccccaa tattcgaccg cattaagttt ttgtattgga
    40261 tttttaatcg gattttgtat ttgcgtactg gctattcttt tcgataacaa atttaaaatc
    40321 attatcagaa aatataaagt gcccctgctt ttggagcttt ctataacaac gttatattac
    40381 gctttgatta ttcctgttgt tttttattcg atcttttata tttttagatt acaggaagaa
    40441 atcagcacat tactttttcc tgttatggta tacagcctag gagccagttt ttttataaac
    40501 atgcttgcga taattattaa tttgatagga ccacccgact ttttacgtct tattagtgga
    40561 cgttattccc gtcccttcga agaagagcga atttttatgt ttattgatat taaaggctct
    40621 acctcccttg ccgaaaacct cggcaacctg aaatttcact ctttcttgaa tgatttcatc
    40681 ttttttatta ccccattaat tttggagtgc aaaggagaga tatataaata tattggtgac
    40741 gaaattatta tttcgtggaa ccccaaaaag ggtttaaagc atttaaactg tctaagaatt
    40801 ttctttttga tagtcaacgc tatacaggac aaaaaaagct atttcgaaac cgaatataac
    40861 aatgttcctg aattcagagc aggtattcat ataggtaaag tgattgttgg tgaaatgggc
    40921 gatatcaaaa aagagatcgc gtatctaggc gatgttgtta acactacagc aagaatagta
    40981 aacgttgctt cggaacgatc tatacctctt ctggggtcag cagaaatcgt aaactttgta
    41041 tcaaacaata aactcaaaac agattatttg tttaccgaaa taaaggaagt aatgttaaga
    41101 ggaaagagta atcagtcagc agtttatcag atcacagaga atattatgtg atcttgttat
    41161 aaatagtttt taagtaaaca caaaacaatg aaaaaaaaaa ttgaaataca aaaactttac
    41221 aaggcattta gagaaaagaa agtattacaa ggaatagacc ttgatatttt cgaaggtgaa
    41281 attatctctg ttgttgggaa gtctggaacc ggaaaatccg ttatactaaa aaatcttatc
    41341 ggattaatga aacccgacac cggcaagatc attgtcgaca atgaagattt cactcaaagc
    41401 tccggagata acttttatcg aatacagcaa aaatttggag ttctttttca aatggcagcc
    41461 cttttcgatt cgatgaatat atttgataat gtcgcttttg gtctaaggcg taaaaaaaat
    41521 gatgagtctt tcataaagaa acgtgtccca gagttattag agcaggttgg cttgaaagat
    41581 atagaaacga aaatgccatc cgaaatatca ggaggtatgc aaaaaagagt cggtctggca
    41641 agagcgctag ctatgaatcc acaaattatg ctctacgatg aacctaccac tggtgtcgac
    41701 ccgattacag gaagtgcggt tgacaaatta atacttaaaa tgaataatgc atttaatatc
    41761 acttctatag tgataacaca tgatcttgaa tcggcattca gaatttctga ccgaattgca
    41821 atgcttcatg aaggaaacct aatttttttg ggatcaccgg atcaatataa aaattcaaat
    41881 gattctgtca tccgccagtt ttctgccgga caggctaacc catcagaaca aacggagata
    41941 caataatgca tagcgaacca cttgacctgc accataaaga aatactctac aataggcttc
    42001 gggcgatttc catcagatta tccgaattta gtttcgcaaa cctgtacctt tttcgtaaaa
    42061 ctcatgggta cgaagtagta cacgatggtg attgtataca tatcacaggt tttacaggag
    42121 acaaacagaa gtttgtactg cctctttgcg aaaaaaaaga accggatcta gagcatatcg
    42181 atgaacttct ggataattat gaaatgattt tccccgtttg cgattgctgg cttcgctatt
    42241 ttgatccccg caactttgat atacaccata acgaagacga ttctgattat atttatactg
    42301 ttgaaaaaat ggccacatac tctggacgac atttgtcaaa aaaaagaaat cttctaaaac
    42361 agtttaataa ctctcatcaa tatactcgtc aaattctgaa caaggataat atcgaaattg
    42421 ctataaaagt tcttactaac tggcaagacg aaacccccgc agatgcatca gatactgatt
    42481 ttcatgcagc catggaggct ctagaaaaca tggaaatact gggactcttt ggatttttgt
    42541 atattattga tgacaagccg ggagcatatg ttttgggtga agacattacc gacgacactt
    42601 ttgtactgca cttcgcaaaa ggactaacag aatttaaggg agtgtatcaa tttatgtaca
    42661 atgatcttgc caaagaaatt cagggtaaat acaattatat taattttgaa caagatcttg
    42721 ggctccaatc tctaaggcag gcaaaatctt cttataaacc tgaccatatg ggaatgaaat
    42781 atcgaattaa aagaagaagg tgtaattagg ttatatattt atttgaaaca tcattgattt
    42841 attaaacaac aatttcactc tcgtttcatg tggaggatac aaatgaaact aaaaactatt
    42901 gcaatatcaa ttgttttcat gt
//
LOCUS       DAOTGZ010000002        42885 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_3064
            NODE_924_length_42885_cov_5.263624, whole genome shotgun sequence.
ACCESSION   DAOTGZ010000002 DAOTGZ010000000
VERSION     DAOTGZ010000002.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868088
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 42885)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 42885)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:22:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,785
            CDSs (total)                      :: 2,751
            Genes (coding)                    :: 2,732
            CDSs (with protein)               :: 2,732
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 12 of 19
            Pseudo Genes (internal stop)      :: 5 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..42885
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_924_length_42885_cov_5.263624"
                     /isolate="AMR_MDS_3064"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..478)
                     /locus_tag="P5123_00180"
     CDS             complement(<1..478)
                     /locus_tag="P5123_00180"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRX14709.1"
                     /translation="MKKTKSLFIAITTLLFIFLIGGLSVYKIYELNFLNSLRLAIESI
                     ERKTATDNEKKINYILNSINNKSSNDIKKIADTLLVQSTDQDKNIIIALKDGTLLGHS
                     KLGEMERLSGNIATDEFVYNIDLIFQPVSINLDSTFQSDYYIIDYKPPFNKKETERL"
     gene            complement(1212..2222)
                     /locus_tag="P5123_00185"
     CDS             complement(1212..2222)
                     /locus_tag="P5123_00185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018249382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRX14710.1"
                     /translation="MILFLRLLFIVLSTAIPALFLISSSAIHAIIAGSAGFILSIPFL
                     ILMEYVSHTCSTRTLISAITGLLTGFSISLLVINGLSMLNLEEYFTFIPFIKTILYHI
                     IGFFIMMVIVINNEEISFFDKIIPAGTGEDDGAISYKILDTSVIIDGRIADICDTGFL
                     EGILVIPNFVLNELQMIADSADSIKRNRGRRGLDILNKMQKDHSIKVKISDMDFYDIN
                     EVDAKLVKLAKVMKAKVVTNDFNLNKVAEFHGVSVLNINQLSNSLKPIVLPGEQMHVA
                     LIKEGKDQNQAIGYLDDGTMIVVENGRRHMNEEVDVLVTSVLQTTAGRMIFARIKDES
                     EQ"
     gene            complement(2446..2928)
                     /locus_tag="P5123_00190"
     CDS             complement(2446..2928)
                     /locus_tag="P5123_00190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002978378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CarD family transcriptional regulator"
                     /protein_id="HRX14711.1"
                     /translation="MFKKGEKVVYPMHGVGLIDEIEDKEVLGQTTKYYIIQIINSGMK
                     VMIPVDKAEEVGLRKIISKSEIKKALSHLSSGETIKENDWKLRYQNNIEKVKSGSIFQ
                     VAEVARDLFRRGREKELSIMERKLYENAYNLVMHEVALTKNVDIEEAGDLVSEALSDS
                     "
     gene            complement(3108..5396)
                     /locus_tag="P5123_00195"
     CDS             complement(3108..5396)
                     /locus_tag="P5123_00195"
                     /inference="COORDINATES: protein motif:HMM:TIGR02074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PBP1A family penicillin-binding protein"
                     /protein_id="HRX14712.1"
                     /translation="MEKESSTTIFGKCIISLLMLGSIIGALIFGYIMSEIKNFTGISN
                     IKQFQLSVPTRLYDVNGELIAELFMEKRELVGFDEIPQTLINALLATEDQAFYNHFGI
                     NFGAIIRAMIKNIQAGEIVQGGSTITQQLAKRLFTESEKTYGRKALEAILALQIERKF
                     SKEEILEMYFNQIYLGHGCYGVATTARLFFDKEVKYLTAAESSVIAALPSAPGRYSPL
                     MNPRNAYEKNRDILNRMVQEGYLTKEQADTIYKEFWPDFIDSLKTEFPTKTAYTKNTD
                     KAPHFTAFIRQVLENRFGRDAVYNDGLIVYTTLDLRRQRSAEYHMKKGLKEQTKLSDS
                     LNAYTRGSVDRGLFGTYGLLRMIFSLPGVVIKHDIESRTRRAILDEVADAADLLSLLT
                     DSRINQEVIENFRSTTALGVVTNLQVEGALVSIEPSTGYISAMVGGSDFNVDNQLNRA
                     VSARRQPGSSFKPFVYGAGIDSHIITSGTTISDAPLVNIDSHGTTWAPGNYGGTYSGL
                     VNIKHALAQSINIIAVRIYDMVGPEKITEFSSRILKVPPFRFNSNPTMSLGTSELTPF
                     EMAGAYAIYANKGRDVIPHGIRLVLDKDGNEIANIEREIGDLLAIKERNNQMQVVSED
                     VAFIMTDLMRGVIDKGTARKKIREEERFTLPAAGKTGTTSNWTDAWFCGFTPDIVTVI
                     WMGYDKPFLSLGKHQAGGVVAAPIWARYMKDVYNGMPKPVFDGPPENVYRNGELYYIK
                     GTHGSGGGARVKMESIFDDYKE"
     gene            complement(5442..5645)
                     /locus_tag="P5123_00200"
     CDS             complement(5442..5645)
                     /locus_tag="P5123_00200"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRX14713.1"
                     /translation="MKKGPLTQFFYAIGVLATIIIAIRYILPLLLKITGWLLSIIITV
                     LIFALVVAAIIYSIAKLSEMIKK"
     gene            complement(5935..7062)
                     /locus_tag="P5123_00205"
     CDS             complement(5935..7062)
                     /locus_tag="P5123_00205"
                     /inference="COORDINATES: protein motif:HMM:NF012318.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trypsin-like peptidase domain-containing
                     protein"
                     /protein_id="HRX14714.1"
                     /translation="MKRKLIAIPVALILLIAAVIYFSCKEPDPENGLIGLDRKSPLRN
                     DSEIRKAWDIQKSFQKIYNLYENRIVFISTEDHVTLPSHPFYELFGIPRIQKQTGLGS
                     GFIISEDGFICTNFHVIAPQNRVVEKITVIIENDTYEAEVRGFSIEKDITILKIEPKE
                     PLNPVFFGDSDTVLVGDWAIAIGNPFGLSKSFTVGVVSATGRKNIDMSDNSSFIQTDA
                     AINPGNSGGPLINIHGEVIGINRLIYSKSGGYMGIGFAIPVNSIKELMIELKDKKYVQ
                     KGFVGIALTPIEKEYASKLGWSHSFGIIVAQVQQGGPAHIAGIRPLDIIYSANGKKIS
                     TIDDFVKIIEDSNPGEKVKISIWRNGRRMNFYVETTSKPAE"
     gene            complement(7135..7794)
                     /locus_tag="P5123_00210"
     CDS             complement(7135..7794)
                     /locus_tag="P5123_00210"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRX14715.1"
                     /translation="MKTIDHLINKNVYYLGNNYEYFKHISRFFALQRCKSIFISNKKE
                     AITSIDRELGNMRATSYAHHVQFNDTEKYIKNLTRILKNGDTPDYLIISFKPEFENFE
                     EGSMPLLNSNKDSIEEITNFYVTNTVLTVKYFLELMVAQGKGKIINITTDTSTNPLER
                     TLFSYLDSHILAVGEDLKSNGYSQIDCKVMHKRASAISYDRFVQKLSNALISSKSIIR
                     L"
     gene            complement(7826..8029)
                     /locus_tag="P5123_00215"
     CDS             complement(7826..8029)
                     /locus_tag="P5123_00215"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRX14716.1"
                     /translation="MKIIFKIMLFISLCFLIYGCSNKSYENRVNINQINADNSNNYYY
                     IFRDQITKERLHARKSEYGAFAD"
     gene            8422..9012
                     /locus_tag="P5123_00220"
     CDS             8422..9012
                     /locus_tag="P5123_00220"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRX14717.1"
                     /translation="MKVVHVLKRIETIDQDIKELKKLEKSIASNKSFSTPIFISIEQQ
                     INLLLDERIKLFGLELANPPQSLLDAVGEEEEEFKPLLNTKTTKKSAKKSSKKSSSAP
                     KKNKQQPKESTDDEDDDLPMRMLTQEQIDAKIDIMSSVEEEKPGLGLDKDKLNDDSVK
                     LLDIALDKGRSSDENVLPKEKKRVRFFKDNFPGGHY"
     gene            9064..10242
                     /locus_tag="P5123_00225"
     CDS             9064..10242
                     /locus_tag="P5123_00225"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRX14718.1"
                     /translation="MKDATIISYPGYHDDYLLSLTGLRSRYMVPFAGRYRVVDFTLRN
                     AFFCKAKNLFIFSRVNDDLAFYVESHPIVKTNKTTVVKTVISEKTEIRMIYDMVKSTQ
                     TAYYILHCGDNPGFINFYKLFEKYQTKKAATILFKLKLGDKASLAYTILITTRKFLLS
                     VLKKALNSNETSPNVFEMIVNMLINKGVLNSTFKATYWPIRSVPDYYDYNMFLFKDQA
                     SFNRIYEDNLLLSGIERDNTTQIGPFAKITRSYISDGCILNGTIDNSIIFPGVIVAER
                     AVVRDSILLPYVGIGQQSVIQRSIIDEFTDYNQSDILFNISEKVRIGSDELQLKNNDF
                     PRSIYNAITLIGKNSIIPSGVRVGAACYIASGVSSSVFDEKKTLYDGLSILNRLVKKE
                     "
     gene            10239..11369
                     /locus_tag="P5123_00230"
     CDS             10239..11369
                     /locus_tag="P5123_00230"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0035438 - cyclic-di-GMP binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PilZ domain-containing protein"
                     /protein_id="HRX14719.1"
                     /translation="MMGEVKKPRRYTASSSEEKISKYIEENRRRDGFHLKSMGKVFPI
                     MFVSYSKKRLEFSSPKQIHSKKLRLYWKNIYHCELDTVAIAEPRPSGEHFIYTVKIRH
                     LLTPESQRFELRYTVPADVYKIGKITIPVVEETAVSILNSDDYQNCKKEMLQKLEYYD
                     FFRIFPFQSAGAPSEVVFSMISNTILYLKDISQYNNFIEKNSAFFDVHKSLRSHLLNR
                     LKTLNLRYHSLLVRPFDYVTIAGSQFTLAYFVVARSSEEITNDDILYLDRIAHDFYQS
                     LQVSLYKVNNCQGRILNLSVTGARIFFKREMVNCISVSSEMSVVFSVVGVADISIAGE
                     VVYIEQKKNGVIIGFVFRKSPFGSRLGKFLHETFQNHGLSIC"
     gene            11393..13021
                     /locus_tag="P5123_00235"
     CDS             11393..13021
                     /locus_tag="P5123_00235"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRX14720.1"
                     /translation="MGFLSKITRIMLFVLFSSVALADEIAVMPYLVETTEVSARNSLD
                     DEYAGLLGIAAAVEYGLDFYSMDNLKKDLKNIGIDYHSILTADDLRLLAKRRMLKYIA
                     TGTIYKTSNGYTVKTVLYDSKTDQIVAKSSVSGRTLFDTAKKDLHDIFRYMPTAKTGS
                     KTVIAESVLLLDCSYNMINEKVALIDSLKKHYETIFRQYPGSKVYCLAFNSQMKSETR
                     SFFTSGALNSYLDNIKFNASNNDKSIRLALDSAVSNIKFQNGSLRYIVFPFNSNMKNI
                     SYFTRFANQVAARRATVYPVVGAKVDISNNAALTSLAERTGGNFYPLLYHQTFFDRKA
                     ESFHLFMRSGRLYFSELPYPQWNSEFKSGEVKQQFDVPTWLNPYNLEKYYRDGLKRVI
                     VKSNPLEVNSRFVFADIIDRFNKKNSRSGYSVTIARVMLSSVNGSVWVDVKDRESLGY
                     FSRLKKSGVYSLIGVRLQVDSDSVYGYSINPKAFVFSVPQDSVPELLKVDLNDLLGSP
                     SSYTEKGLLEPPVWFINVRVQMIDLKTDVKDIRS"
     gene            13262..14494
                     /locus_tag="P5123_00240"
     CDS             13262..14494
                     /locus_tag="P5123_00240"
                     /inference="COORDINATES: protein motif:HMM:NF024686.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chitobiase/beta-hexosaminidase C-terminal
                     domain-containing protein"
                     /protein_id="HRX14721.1"
                     /translation="MRIFAGFIIILIFMPVFAQSRQSVVITDFYNTSRIKTSDHYSTI
                     VPDNIRTELDRTGYYSVKRTVARNIELDEDGIKKAAVESNADYFITGEYTSRGGEIGV
                     SAHVYVKNIDKVVTIVMEPKRVGVFLNEIIDNISLKIGFELQKYRDRSGEMPVIEPAS
                     SDFSYYAEIIISSPFPDMPVYYTLDGTLPGPGNGFRYSNPFTVYNETVVKAVSIDKEG
                     VPSDVAVKNFRPLFVPSVFDFKILYGVMQFVSSGDDVESPIDTTIISVVPVFYLGGFD
                     TFKKTAVARNLGFGGWFDFANADLDGGYQYKIQGYSGGVFLRFNPFNNLSLEIPFTVG
                     KMRTSLIEEMSVYENIFSEPGDTLASDLETYCNSGIVATYRVSNFQFIASATMKYILS
                     DDDPTKILVYQAGAGVCY"
     gene            14481..15224
                     /locus_tag="P5123_00245"
     CDS             14481..15224
                     /locus_tag="P5123_00245"
                     /EC_number="2.7.1.33"
                     /inference="COORDINATES: protein motif:HMM:TIGR00671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004594 - pantothenate kinase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type III pantothenate kinase"
                     /protein_id="HRX14722.1"
                     /translation="MSATDLLLFDCGNSTINSAISRNGEFPDNFFTFNHKDNDIEDQL
                     VSYIRKNGIFRAVFVSVVPMLTQTLLSVFDKEGVACIEFYADQCGVSSLYTNPGSDRI
                     LFAYSIKTFYPGRAMCLIDAGTALTVDGLDRSGRFLGGFIMPGVKTAFTSIARGAALL
                     SGYELSISHSVGIDTESSFGSGLSVLYSGGLKAAIEKISALCGDKVDVVLTGGDGPLV
                     KELLSDCLLVYDPLILLKGLVVYYYNCSH"
     gene            15383..15829
                     /locus_tag="P5123_00250"
     CDS             15383..15829
                     /locus_tag="P5123_00250"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRX14723.1"
                     /translation="MNQVKKDLFMSLAEQTALRSGIEIYDIVVQFRKGVVRVDIRIDN
                     GATVSHNDCSLFCTDYIEVLKEHGLENEAAVEVSSPGLKRKIRNHKEWLRFEGAPVKV
                     TYSDEKGSVTVKGVAKEVLKDSVVVVENKKKHVIGYDTIRHANLDY"
     gene            15844..17163
                     /gene="nusA"
                     /locus_tag="P5123_00255"
     CDS             15844..17163
                     /gene="nusA"
                     /locus_tag="P5123_00255"
                     /inference="COORDINATES: protein motif:HMM:TIGR01953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006353 - DNA-templated transcription
                     termination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination factor NusA"
                     /protein_id="HRX14724.1"
                     /translation="MNVNFFEALHQIATEKGISGDELEQIVEASMVSAYKKQFGTTDN
                     MRVVFDRDKKTIAIASLKLVVRKPQNPGEEVGLDVAREVKPDIQIGDSIEMNDDPFKL
                     FGRIATQTAKQVIIQRIKEAEKNIIFVEFKAKEKQLINGFMQRRSNNTIFVDIGHGGV
                     EGILPRREQSPLEFYKEGDRIKALVLNVENKTKGPNIILSRVAPDFIKELFKMEIPEV
                     YDGIVEIKRVVRDPGMRTKVAVTSYERDIDAVGACVGMKGSPIQAIVRELEGEKIDII
                     EYSTEKRVLAENALTPARINEIYETRDGGLIAVVTSDQYNLAVGRAGHNVRLASQLCG
                     VEVRVKTEEQYRELVESDDSMLMSNQLFSEPVEDETSLTELDLDSRTIELLEKGGIFS
                     VEELVESTIDDIKNIDGIGEKTARRIFEILAEVVDFEDEEDEEDEEE"
     gene            17261..19855
                     /gene="infB"
                     /locus_tag="P5123_00260"
     CDS             17261..19855
                     /gene="infB"
                     /locus_tag="P5123_00260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013254614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003743 - translation initiation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006413 - translational initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-2"
                     /protein_id="HRX14725.1"
                     /translation="MKAEEIAKKNLVKTEDVFSICKDLGIECKDAESSLSDSDTFLIE
                     KKIETIIAEKKAKTQKKASTGVKKKIKLKRKVSADAESHVISEIKKHSDETAPEKTGA
                     DKTSQPKSAVKKSSERPADKSSASASRPVQGRPAASSSSRSNTARPQGSDSRRPASGN
                     RPTQGTRPAQGTRPGQAARPTQGARPAQGARPGQSSTGQRRPTGAGAAPFTKSTQEVP
                     VASEVGDKSGKSKRRNRERDKERENLKNKRSEKLDREQRVYNSHITKKRKPVVKQVRE
                     AVTPDSIEITENISVGDLAKKLNVKANEVIGKLMKLGVLATINQILDSETAEILAEEY
                     GTSVKVVSLYDETVIKEDEIQKDIDVIQRPPIVTVMGHVDHGKTKLLDTIRKSNVTAG
                     EHGGITQHIGAYKVKVHDQWVTFLDTPGHAAFTTMRARGARVTDIVILIVAANDGVMP
                     QTVEAINHAKEANVPIIVAINKIDLPEANPAKVRQELAGYDLVPEEWGGTTLYCEISA
                     KNDINVNALLEQVLMQAEILELKANPRLRAKGSVIEAKLDPGRGAVATLLVETGTLHV
                     GDSFVVGIYAGKIRAMFDDQGNSVAEAGPAMPVEILGLDGIPSAGDPFQVVASEKYAK
                     QIAGKRQELRRVESAKQVKKVSLEDLNDMIKEGEVQDVRLIIKADVDGSVQALKDALE
                     KLSTDEVRVKVIHAAAGGINESDVMLASASNAIVIGYHVRPTPKVSDLADQEKVSIKT
                     YNIIFEVTQDIKSAMEGMLKPDIEEESTGMAEVMQVFKISKLGTIAGCTVIKGKVHRT
                     NKVRLLRDGVVLFDGDLKSLKRFKDDANEVEMGQECGIGLNKFNDIKVGDIFESYRLV
                     EIAKKLGD"
     gene            19857..20222
                     /gene="rbfA"
                     /locus_tag="P5123_00265"
     CDS             19857..20222
                     /gene="rbfA"
                     /locus_tag="P5123_00265"
                     /inference="COORDINATES: protein motif:HMM:TIGR00082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosome-binding factor RbfA"
                     /protein_id="HRX14726.1"
                     /translation="MSSYRKEKLEELIKRLVAGLLLKEIKDSRIGFISISRVEISKDY
                     SSANVFFSVLGSEKNIKDSFFGLESAKGFIRKKIGKSLKLRHIPDLKFVYDDSVAEGV
                     NLVNLIDKVNAPKADSPDE"
     gene            20215..21120
                     /gene="truB"
                     /locus_tag="P5123_00270"
     CDS             20215..21120
                     /gene="truB"
                     /locus_tag="P5123_00270"
                     /EC_number="5.4.99.25"
                     /inference="COORDINATES: protein motif:HMM:TIGR00431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004730 - pseudouridylate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(55) synthase TruB"
                     /protein_id="HRX14727.1"
                     /translation="MNKTAFNDKVILIDKQKGLTSFDTISRLKKKVGIKKIGHSGTLD
                     RLASGLLVVATGKATKLTRYFLDSDKTYNAVVQLGIVTDTLDADGTVVSSASVTTEHM
                     AGVCAVLDSFVGKIEQVPPLYSALKIDGKRVSDRVRDGEALTLGSRSVNVYSIDLIST
                     DPVRSTFEMRVQCSKGTYIRSLARDIGQKLGCGASILDLRRTASGEFSVEQAVSVENV
                     SADADERNYIVTIESALKFMNQVTVDKSAVTRIMNGAWFDEGSLLSVDNRGSDYCAVS
                     DGKMVLAIAKINFNDWHVVYENVFN"
     gene            21303..21560
                     /gene="rpsO"
                     /locus_tag="P5123_00275"
     CDS             21303..21560
                     /gene="rpsO"
                     /locus_tag="P5123_00275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013290756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S15"
                     /protein_id="HRX14728.1"
                     /translation="MKEKKEVITPYKRHDSDTGSPEVQIALLTERINHLTEHFKVHKK
                     DFHSRRGLLKMVGQRRRLLDYLKKKDLGKYRELIASLGIRK"
     gene            21587..23671
                     /gene="pnp"
                     /locus_tag="P5123_00280"
     CDS             21587..23671
                     /gene="pnp"
                     /locus_tag="P5123_00280"
                     /EC_number="2.7.7.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014803154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004654 - polyribonucleotide
                     nucleotidyltransferase activity [Evidence IEA];
                     GO_process: GO:0006402 - mRNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyribonucleotide nucleotidyltransferase"
                     /protein_id="HRX14729.1"
                     /translation="MSHDYEVEVGGKKIIFSTGEMAKQAHGSVTVRLGDTMILATAVA
                     APSASEGRDFFPLQIEYKEKYYASGRIPGGYVKREGRPGDHEVLICRITDRPLRPLFP
                     KDFLNEVQIAITVISSDKKELADVLAINGASAALSISGIPFHGPVGAVRIGRIDGNFI
                     VNPTVVQTEESDIDLMVAGTERAVTMIEGSSKNISEADMLAAVECAHENIKIICAAQK
                     QLMEKHAKPFMEYKPKEKNNELASIVREKYYAEVEALMNIHMKKEREAAYNAIVEKAK
                     AELEERFCDDIGEVEPIIHDMDKDVVRQAILDGKRTDGRSHDQIRPITIRTSVLPGAH
                     GSALFTRGETQSLGTVTLGTEKDSMRIDSMNGDANRYFFLHYNFPPYSVGECGRFGGT
                     GRREIGHGMLAERALTYAIPDIKDFPYTVRVVSEILESNGSSSMASICSGSLALYDAG
                     VKTKSPIAGIAMGLVLEGDRHAVLSDIQGLEDHLGDMDFKVAGTEDGITAFQLDIKIE
                     GITSEIMREALEQARKGRLHILSEMSKALPVPREELKPNAPRISEIKIPTDKIGELIG
                     TGGKNIKGIIEETGSEINVSEDGTVRIYSSDQSILNATKELVERSIAEVEKMKIYEGT
                     VKRIADFGAFVEILPGKEGLLHISKIDHKRIKQVTDVLQVGDKVKVKVMNVDKGGRMD
                     LSRKDALPPESE"
     gene            23685..24953
                     /locus_tag="P5123_00285"
     CDS             23685..24953
                     /locus_tag="P5123_00285"
                     /inference="COORDINATES: protein motif:HMM:NF017044.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pitrilysin family protein"
                     /protein_id="HRX14730.1"
                     /translation="MKRYTLANGLVVIMQPIEHAVSASVGLWVKTGSRDELPEQYGYA
                     HFVEHMLFKGTPRYTAKELAQLVDRIGGQHNAATNKEYTCYYLNVVSDYCVTAVDVLA
                     DMFYNPLFIDAEIEKEKGVVIEEIGMYEDTPDEHIHDLFTSVMFNGHPLAHLILGTNE
                     SVSSISRNTITSFYDSCYGTGNSVLVVAGCFDLEEITLKIEECFAQKRGYEKNIDLSS
                     VYSQMNTERIYRHHVDRELEQVHFCLGVDGYKKQVADRWALYLVSTILGGSMSSRLFQ
                     RVREDEGLCYSVYSFHSSYRDTGAFGVYCGTSSEKYERALKLILDELDIIVNKGISES
                     ELDDAKRFMKGNLALSLESIEVRMGQLARDEIHYGAFSSYDDIVKNIFSVTTADFDRV
                     VRDLFNNKRISLVSIGNKVKYDDRPLYIGQ"
     gene            24963..25370
                     /locus_tag="P5123_00290"
     CDS             24963..25370
                     /locus_tag="P5123_00290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008593196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polymer-forming cytoskeletal protein"
                     /protein_id="HRX14731.1"
                     /translation="MAPRIDSLNSTIGDGSVFEGKFYINGSLKIDGKFEGEIKTDEEL
                     VVGETGKVRTNIEAKSVTVAGTVIGNIKALNEVRLLETAKVLGDIEAPNVSVQKGVVL
                     TGRINICGEYKKDLEKVVEDSYNSTPLEKYKKQ"
     gene            25488..26486
                     /locus_tag="P5123_00295"
     CDS             25488..26486
                     /locus_tag="P5123_00295"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: protein motif:HMM:NF013700.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M23 family metallopeptidase"
                     /protein_id="HRX14732.1"
                     /translation="MQKFTIRKLSVSRIHNEIMFVYRGKENIYVKKFTFSVNRLYHRL
                     LYFVGASVLTIALVIALPFGGKNADSSTPEDEELKNDILLSDETDFTEQDKRKPLAVQ
                     IYKVQQGDTLSEIAAKFGVSTDTICGSSGLTSYDFLKIGQVLKIPDRDGMLVTVKEGE
                     KLIDIAQKYKIPVENIMAHNQLLNPDFLPVGYDIFLPDAKPQNIIKGFMWPTFSRRIT
                     SAYGWRIHPISGRRQFHHGVDIRSRYQDIKAAKYGKVTFAGSMGGYGRAIIIAHPGNQ
                     KTLYAHLSKLYVKSGQYVRQGQMIARSGNSGYSAGPHLHFEIIENGKHINPRRHLK"
     gene            26623..28698
                     /gene="glyS"
                     /locus_tag="P5123_00300"
     CDS             26623..28698
                     /gene="glyS"
                     /locus_tag="P5123_00300"
                     /EC_number="6.1.1.14"
                     /inference="COORDINATES: protein motif:HMM:TIGR00211.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009345 - glycine-tRNA ligase complex
                     [Evidence IEA];
                     GO_function: GO:0004820 - glycine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006426 - glycyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine--tRNA ligase subunit beta"
                     /protein_id="HRX14733.1"
                     /translation="MWNRINFLLEIGSEEIPAGYISPACQVYSDLFEKKFNDASIKFG
                     AIHTFATPRRMVIAIEELADKQLSETVEVRGPAVEKAFDDNGKPTKALEGFLKASGAT
                     IDDIDKRDTGKGIYVFVNKTTQAGKTVDLLPSMIEEIIGSANFPKKMKWSNKSISYPR
                     PLRYLLTLFNDKCVEYSIDGINSNNKTRGHYIQHNGMIEVASIAGYPDLLKKNGVILD
                     QQERKQIIKNALVDTAKSLDAVLIEDEELFETVTYLVEDVNIVVCSFDKEFLQIPDIV
                     LIAEMKEHQKYFALRQKDGSLLNRFCVVSNNPATDFVKEGNERVIAARFSDAEFFYKE
                     DRKHKLADKVDMLKSVLFHKDLGSVYDKVERVRAVSREFVKRLKIDNNIAGKIDRAVT
                     LCKADLNTSMVFEFTSLQGKIGAIYALLDGEDFDVASAIDNHYRPRFQGDEKPSDIIS
                     CVLSLSEKVDNLFGAWSVGNVPKGSQDPYALRRQAYAIVDLLIYNKINIDLNNLFEVV
                     ALNYKDGAQLIEPILDFISVRAKTRFQEDNIQYDEIDAVLTTGSRDYYELYNRAASIH
                     EFRSRNDFSKMLASFKRMNNIITSYLKKNKDYQFGFDRNKCSTRQETALVDYFLDKKD
                     VISDLSSKHEYTKIFELLIGAAGIIDDFFDNVMVIDEDPVKRDLRLGLLNEILMLFSA
                     FIDFSRISD"
     gene            28703..29236
                     /locus_tag="P5123_00305"
     CDS             28703..29236
                     /locus_tag="P5123_00305"
                     /inference="COORDINATES: protein motif:HMM:NF014614.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cob(I)yrinic acid a,c-diamide
                     adenosyltransferase"
                     /protein_id="HRX14734.1"
                     /translation="MTTDNNLNGRVHAYVGNGKGKTTAACGLMMRTAGYGKLAVMVQF
                     LKKRQTGESMFAGWTNNIVIRQFGTEDFYRPGKSDILLYRRLCRQGLDFAIEKAVDAS
                     LIVLDEVLDAVDYGLIVIDDLFYLLHDCTLNGTEIILTGRKVDDTLANHCDLITEMTS
                     VRHYYDKGISAREGVEF"
     gene            29236..30219
                     /locus_tag="P5123_00310"
     CDS             29236..30219
                     /locus_tag="P5123_00310"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRX14735.1"
                     /translation="MRLLLVENEFSELSSFLNTHGYGVERTSASSFFDMLKVRQDFNF
                     IVLYMEECDKETASLIISYCLNNSLCFVVVSSVFTDELASWLKEHGVARFVTVDEIFR
                     NSQIFRFVLAYDDIEQLRIVHLGSDLASHRIIESISNSYGVYYLWVNDEKSLIDNVDC
                     STDMVLLDIGCVDFDTQHFLHKALYNRDFLKTICVPFSFNGDISITDINCGLNRLAKV
                     FLNLDELLGFLARYYHERELVCAMHALKEFVQKYCEDGCLSKSSLQVYHEKGDSFFEH
                     FLPDDDYFEGMRERSQRLNAVGYKSSMFRWLLSGWSRGKNGSQNVYMQIRD"
     gene            30311..30574
                     /locus_tag="P5123_00315"
     CDS             30311..30574
                     /locus_tag="P5123_00315"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRX14736.1"
                     /translation="MKVGEFLLENGLINSDQLDEALLLQKDNPDRVIGEILVTMGVFS
                     KEELIMAMEMYMVSTGFDLNFIDEWLDQDEIDLLSEKIDKQKM"
     gene            30646..31062
                     /locus_tag="P5123_00320"
     CDS             30646..31062
                     /locus_tag="P5123_00320"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017862332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047617 - acyl-CoA hydrolase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PaaI family thioesterase"
                     /protein_id="HRX14737.1"
                     /translation="MIEYEDKTCFGCGEGNPRGLQLKLKYDPDTKTAYGEYTAGQDLE
                     GPPNIIHAGILAAILDETMIMVNKNMEVVALTGEITVRYLQPAFIEENLYIRGWHVKK
                     NKRVIENRAEIENEMGKIVARAKGKYLEVDEIPQPE"
     gene            31067..31150
                     /locus_tag="P5123_00325"
     tRNA            31067..31150
                     /locus_tag="P5123_00325"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:31101..31103,aa:Leu,seq:caa)
     gene            31306..32829
                     /locus_tag="P5123_00330"
     CDS             31306..32829
                     /locus_tag="P5123_00330"
                     /inference="COORDINATES: protein motif:HMM:NF019344.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HRX14738.1"
                     /translation="MSVSSSDNQKKFDEFFQSMEQDIFANANSVQKDEGKPVKETEFS
                     FDLKPEELETYLNKYVVGQQNSIEVIATKVCTHFNRMKLEKELSDDERVTGSIKSNIL
                     LIGPTGVGKTYIIKLIADKIGVPFVKADATKFSETGYVGGDVEDLVRELVHEADEDIS
                     KAEYGIIYLDEIDKIASSSSGNGIDVSRSGVQRNLLKLMEEADVDLKTPHDLASQVEA
                     AMEAQRTGHVKRKKINTRNILFICSGAFSGLQDIIKKRMNLQAIGFDSGNSDKKAFLE
                     TEILAETRTEDLLEYGFESEFIGRLPVTVVLNDVNEDVLYQILKNRYSTVVNGKKLDF
                     LAYGIRLFFEDEALRILAKRAASEHTGARGLLSVFEKVLIKYEKIMPSLHYEELVVDA
                     DLVNNPEKKLKELLLNDNIKGFQSDFLAAHGIYLDFSDEAMELIQQRASVEHKSFKAI
                     CEDLFRDYAYAIKLLKREQFTITAEAVQDSAGYIEKYIKDNYKKSSGNASQDDTDFV"
     gene            complement(32884..33333)
                     /locus_tag="P5123_00335"
     CDS             complement(32884..33333)
                     /locus_tag="P5123_00335"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRX14739.1"
                     /translation="MKKLTIAIFIVCLNINAAEQSVVHKKIIDHLSYLGYLAKINNGT
                     IIVKHPTKPGFDIIQTRGGIVLRAWFKHKKNSLSQKTAYLELVNRINISSIVSKYTID
                     SQNDLVITACYPLPYYKESFSVFISAWETDFIKNSTAYAEELGKYIE"
     gene            33461..34504
                     /locus_tag="P5123_00340"
     CDS             33461..34504
                     /locus_tag="P5123_00340"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013700.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M23 family metallopeptidase"
                     /protein_id="HRX14740.1"
                     /translation="MFGSYKKIVFLVFLVTISSLSSQEKSVDRPRLIDKTSLKAPASF
                     TKQSVKSAVFDIHMARVTLCSRSFEQGDLAYLEIVPQDESNLDSIKITYYGKKKSKRT
                     IAYIPVTRLKWGYRAFIPLDPDSDPGKHTVSLYYKANGREVQKNLVFKVLKRNFEKST
                     YPLYFKGYSTAGKLKPETKKFIEESRIKKDKAFASREDDYINNSLSHPRDMHYITSSF
                     WAKRTYKRYRRLKNGKRKRLKDTVKIHRGIDLRGKRGTPVYAMARGKIVLADKLYYEG
                     NMVIIDHGNRIFSYYMHLDSFSVNEGDIVSSGCELGTVGSTGRSTAPHLHVSLVVAGV
                     QVDPLSLLVLPIR"
     gene            34662..34958
                     /locus_tag="P5123_00345"
     CDS             34662..34958
                     /locus_tag="P5123_00345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013646029.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015036 - disulfide oxidoreductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutaredoxin family protein"
                     /protein_id="HRX14741.1"
                     /translation="MKFDNFTRVEGDSCGTIQLFGLSTCIWCKKTKEYLNSKGIEYYY
                     VFVDLLNESEKEEAKKLIKKWNNDISYPTIIINESRSVIGFDEDEIDREIARNE"
     gene            34951..35466
                     /locus_tag="P5123_00350"
     CDS             34951..35466
                     /locus_tag="P5123_00350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013824720.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016730 - oxidoreductase activity, acting
                     on iron-sulfur proteins as donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin-thioredoxin reductase catalytic
                     domain-containing protein"
                     /protein_id="HRX14742.1"
                     /translation="MSKNNSNLEDSEIDGLYRIVERDAHEGGYLLNPDDSFTRELIRG
                     LVVNTKRYGYAACPCRLASGDEAEDRDMVCPCDYRDADLNEYGACYCALYVTEEVASG
                     KRKLRPLPDRRERELEKKKKDKGESEKNISGLSYPVWRCSVCGYLAARSMPPEKCPIC
                     KVSKERFEKFI"
     gene            35554..35856
                     /locus_tag="P5123_00355"
     CDS             35554..35856
                     /locus_tag="P5123_00355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011590400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Dabb family protein"
                     /protein_id="HRX14743.1"
                     /translation="MIKHIVMWKLKDDVDGLTKDQTALLLKKEIELMKEKIPQIVKIE
                     AGINYNHGPIAYDLCLYSEFENDDDLAIYQDHPDHAVVKELVVKYAENGVVVDYEI"
     gene            35960..36478
                     /locus_tag="P5123_00360"
     CDS             35960..36478
                     /locus_tag="P5123_00360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011628671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HRX14744.1"
                     /translation="MNGEIVITLDSEKAPLSAENFISYVKDGFFDGTIFHRVIPGFVV
                     QGGGFTQEMDQKKTKDPVKNEADNGLSNVRGSVAMARTQIVDSATSQFYINLKDNVML
                     DHKGKDEQNYGYCVFGHITSGMEFVDKIAGVKTGNSGFHRDVPEIPVVIKSAVLGDNG
                     KTVTFTIEQATE"
     gene            36484..37569
                     /locus_tag="P5123_00365"
     CDS             36484..37569
                     /locus_tag="P5123_00365"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009975 - cyclase activity [Evidence IEA];
                     GO_process: GO:0009966 - regulation of signal transduction
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HRX14745.1"
                     /translation="MENRDIEQYFKEMMSAEKSADHSIITSLEKKVYDMKNLIEIGIS
                     LSSNLDFKSLVDAVLYSCIGQLFIDKIAILLQVDIDSNCYYIHSHKGYDITSISDEII
                     LGENSVLSSFLQDHPEPHDYEALKKIPELSEDLSAVDDLSPILIVPMKSKNSLNGIII
                     VGNKMLGTPFTTDEKEFLRQLSRFSATAVENSRLYLMATQDRMTRLHVHHFFQDKLLE
                     EMKRSQRSGEPLSLFITDIDHFKKFNDVYGHQQGDTVLKEVARLMRESVRATDCAARY
                     GGEEFAVILPGASLELASDVANRLRKTIEQHEFPGQNEPLHVAISAGVAQYDPECDLH
                     KEDLIERADKALYKAKESGRNRVAVFR"
     gene            complement(37687..38097)
                     /locus_tag="P5123_00370"
     CDS             complement(37687..38097)
                     /locus_tag="P5123_00370"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRX14746.1"
                     /translation="MKKLFTITSIILALLIFISCEDDESETDGIKNAVLDLQDAYNSG
                     SEAEITVLFSEGSNAGGSGIASMETLKDSVGTANLEFTDISTSESGDTGSATAYHDDD
                     LGMEHKNTFTLVKSGEYWDFTEWIIDGTTTLDRK"
     gene            complement(38124..39164)
                     /locus_tag="P5123_00375"
     CDS             complement(38124..39164)
                     /locus_tag="P5123_00375"
                     /inference="COORDINATES: protein motif:HMM:NF016648.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FecR family protein"
                     /protein_id="HRX14747.1"
                     /translation="MITVKKLLLILFAMTFSLPAFSAMQIKFFVGEAKIKPFGSDTWQ
                     KASMNQKLVNGDTVKTGSKAYIQIDMDGNTIKVNENTQLAIGQDIDEEKKVQSLDMTR
                     GTVQLKMDKLQNNNSGFKVNTVSTVCAVRGTEFLVAAGYDGTSVLQVQKGRVAITGSS
                     RTVLVNANQETTVPYGGDPSEVEDIKISEWNKWISDSKNQLKGNEESVLNESLKKMKK
                     LDNDITELEEIKEFNQKEKIKYDELAKKFKADGNTAKFEENAKLSFQAAQKLHLASFR
                     AIVNAYKMGMVKDFSDLTYETIDRSNRTAEINKLYMEIDDLYKKYYIKYIKAAVEAKQ
                     KRDELRKKRSRR"
     gene            39551..40480
                     /locus_tag="P5123_00380"
     CDS             39551..40480
                     /locus_tag="P5123_00380"
                     /inference="COORDINATES: protein motif:HMM:NF012579.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HRX14748.1"
                     /translation="MEKVEIKSADFTAFFTAEDIICHSDLKKRNDLFSAILKNLAYNK
                     GIGNVNDAYNAVLEREEGYSTVLSKGLALPHARLSTISELVIGIATSKEGIVFDGDSE
                     NPVHVVIMILTPKDKPSLYLQVLSSVSKVLAQEGIIDKLAEFSDNNEMWRFLNRSGLD
                     FPDYICAGDIMNSDFSSLKENATLEQAIDLFVNKNMNDVPVLDADGDLVGIVSTSELL
                     RVCLPDYILWMDDLSPIINFEPFSHILQNESNTWLAEIMSYDFASVDEKDPAIMVAQE
                     ITKHKKGQAYVLRDKKLVGVITMQQFLNKILRE"
     gene            40484..41905
                     /locus_tag="P5123_00385"
     CDS             40484..41905
                     /locus_tag="P5123_00385"
                     /inference="COORDINATES: protein motif:HMM:NF015557.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SLC13 family permease"
                     /protein_id="HRX14749.1"
                     /translation="MHSDNEKITPRQSMIRGGILLAVTFFAGWLAYYAGLTENSQVMA
                     ASVFVLIIGATLLFWNARLAIAFIGIAILLGGRVLTLEQMIVSAELDIILFLVGMMTM
                     VGVLKDLGLFTWIIQLVINMKAMNGKLFCIIVVVMSAVMASVVDEVTSIVFILALIFQ
                     VCDTLKINPVPFVMIAVIGTNIGSSGTMLGNPVGILIGTKAGFSFADFVRWAAPVMFV
                     SLLAALFIVLVWYRKDIQLLTERLEARRKMQLGLGPLVRIPFKRGLFILVSAVTLIAL
                     HHKLETLLGVQKNTLLIVAPLAISGILMVWRSNRARHYIEAEVEWWTLLFFMMLFAVA
                     GSLEHTGVTEHVAHNFVGTFGNNPVVLTPIIIFVTALGSAFVDNIVFVAAFIPVIKEL
                     NMTPLWWAMLFGACFGGNITAIGSTANIVALGMMEKRYKKHIAFFEWIKIGAVVGFVT
                     CVVAWILILLLMPYMPEMNQIGH"
     gene            41966..>42885
                     /locus_tag="P5123_00390"
     CDS             41966..>42885
                     /locus_tag="P5123_00390"
                     /inference="COORDINATES: protein motif:HMM:NF015892.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HRX14750.1"
                     /translation="MKYLLVRGYYTDLFRKFFFIFIAVSLFCLNCCTGNVERDSEVLS
                     RNIVETVNGIIYEFKGNKNTLVFYLEGSGLHSVLGVKKNDGWSHRTFSFFVIKYFNAF
                     ADVVVIEKPKMKPGKDHSTDGEVLVAYTSSNLIDHYSVMIDDYLDKSDAKQVVIFGYS
                     EGGMLAPAVYRNLKNSDRINKLVIGGAGGMSQYEQFMVLAESYVKMPDKYRLECRDIQ
                     RVRSRVRTFPDSIEFSFLGLPYRRWNSFFDFSPADYYANIEIPVLFYHGTFDWSVPVE
                     STSMLEKLYPDKNFNFMYIDKMQHVPEDDKG"
BASE COUNT        13113 a         6835 c         9113 g        13824 t
ORIGIN      
        1 tgagtctttc agtttccttt ttgttaaacg gaggtttgta gtcaataatg taatagtctg
       61 attgaaaagt cgagtcaaga ttgattgata ctggttggaa aataagatct atattataca
      121 caaattcgtc cgtggcaata tttcccgaca atctttccat ttctccgagt ttggagtgcc
      181 cgagcagagt accgtctttg agtgcgataa taatattttt gtcttggtct gttgactgca
      241 cgagcagggt gtctgctatt ttttttatat cattactgga cttattgtta attgagttta
      301 aaatataatt aatttttttt tcgttatcgg ttgctgtttt tctttcaatt gattctatcg
      361 caagacgtaa agaattaaga aaatttagtt catatatttt ataaacggat aagcctccta
      421 ttaaaaaaat aaaaagaagg gtagttattg cgataaataa agattttgtt tttttcatga
      481 ctgtttcttc tttaatggga ttactgtatg tactgttaat agtatcggtc attataatta
      541 tttacacaag aaaagttata atatttcctt attgccgaaa attaaaaaaa ttgatttcta
      601 ctttatatta tcaggccgtt cttttctaga atatattact attttacagt ttcttgcaac
      661 aaaaatagta ctaaatcagt aacgaaagtt aatttgtagt ttaaagaatt gatccaaatt
      721 tctgaggaac ggtatcagta ttaaagtaga actcagagtt ctctctgtcg tatcttatac
      781 caatagtatt gaagaaatgc cttaatatgg ttatcgatgg agagaaaata ccggggtaaa
      841 atgatccagc gcttaaaaag actatacctg atctttctgg gttttttgaa aatttcttcc
      901 aaaatagagg ttgtgtgcgg tcgataaaag ctttcaggtg agaggggagt gaactgaaat
      961 atatgggtga acttataaaa atatttttac aattttctag aattggataa aatttctgca
     1021 tatcatcgtt tattacgcat tttccagaag tggcacacaa accacaaccg ttacaggggg
     1081 atatgttgta ttgtgacaga ttacagtgta taatatcagg ggtctcttta tttgctaacg
     1141 aattaacatg gttgtccagc agtgatgaag aaaaggatga tttggccgga cttccataaa
     1201 ttgctaagat tttattgttc agattcatct tttatacgag caaatatcat tcttcctgca
     1261 gtagtctgga gaacagaagt aaccaggaca tcaacttctt cgttcatatg acggcgaccg
     1321 ttttcaacta ctatcattgt tccgtcatca aggtagccta ttgcctggtt ttgatctttg
     1381 ccttccttga ttagcgctac atgcatctgt tcacccggca aaactatggg tttgagagaa
     1441 ttagagagct ggttaatatt taaaacggaa actccgtgaa actcggcaac tttgttaaga
     1501 ttaaaatcgt tggtaacaac cttagctttc attaccttgg ccagttttac aagtttggca
     1561 tcaacttcgt taatatcgta aaaatccatg tcagaaatct tgactttgat tgagtggtct
     1621 ttttgcattt tatttaaaat atccagcccg cgtctgccgc ggttgcgttt gatgctatca
     1681 gcagaatcag caatcatttg cagttcattc aaaacaaagt ttgggataac aaggatgcct
     1741 tcaagaaatc ctgtatcgca gatgtcagca atacgtccgt caataataac gcttgtatcg
     1801 aggattttgt atgatattgc accatcatct tcgcctgttc ctgctggaat gatcttgtca
     1861 aagaaagata tttcttcgtt atttattaca ataaccatca ttatgaaaaa tccgataatg
     1921 tgatacagga tagttttaat aaaaggaatg aatgtaaagt actcttcaag gttaagcatt
     1981 gagagaccgt taataaccag caaagagatt gaaaaaccgg tcaaaaggcc ggttatcgca
     2041 gaaattaaag ttcttgtact acaagtatgt gatacatact ccataagtat cagaaaaggg
     2101 atagacaaga taaatccggc agatcctgcg attattgcat gtattgcgga tgatgagatg
     2161 agaaaaaggg ctggaatagc cgtactaagt actatgaaca ataggcgtaa aaaaagtatc
     2221 atataattgc tcctgattta ataaataggt atagtcaaat attctaaatt tcttggaagt
     2281 gaccggaatt ttgatcaaag caaaaacatc ggtaatctat tagtgtcaga ttacctgata
     2341 ttttgcaatt ataaaaatta taagaagaag aaagttggtg gtgtagaaat aatcaggcag
     2401 ctttatgagt taacagttta aaggttcaat gactcaggat ggtttttaac tgtctgacag
     2461 tgcttcagaa accagatcac ccgcttcttc tatgtctaca ttttttgtta atgcaacttc
     2521 gtgcataacc agattatagg cgttttcata tagtttgcgt tccatgatgg aaagttcttt
     2581 ctcgcgccct ctacgaaaaa gatcacgggc gacttcagct acctgaaata ttgaaccgct
     2641 ttttactttt tcaatattgt tttggtatcg taatttccaa tcgttttctt ttattgtctc
     2701 acccgagctt agatgggata gggctttttt tatctctgat ttggaaataa ttttgcgtaa
     2761 acctacctct tcagccttat cgacaggaat catcaccttc attccgctat ttattatctg
     2821 tatgatatag tatttcgttg tttgacctaa cacttcctta tcttctattt catctataag
     2881 acccaccccg tgcatggggt atacaacctt ttcgcctttt ttgaacattc agttctccct
     2941 ctgataaacg gtataaaaaa atttaagcgg gtaatgatgt agcatttttg attgaaatcc
     3001 cgtttggtta gccattataa aaaaaatggg ttaaaaaatc aagatattca ttgatgttca
     3061 tgcaaataat tgattaaaaa tgtataatat aatgaattat ctaaaatcta ttccttgtaa
     3121 tcgtcaaaaa ttgattccat ttttactctt gctccaccac cggaaccatg tgttcccttg
     3181 atatagtaaa gctctccgtt acgatataca ttttcgggag ggccatcgaa aacaggttta
     3241 ggcatgccgt tatagacatc tttcatgtat cttgcccaga ttggagcggc aacaactccc
     3301 cctgcctgat gtttccctag agacaaaaat ggtttatcat accccatcca tattacagtt
     3361 acaatatcgg gagtgaaacc gcaaaaccaa gcatcggtcc agttggaagt cgttcccgtt
     3421 tttcccgcag ctggaagtgt aaatctctct tcttcgcgaa tttttttcct agctgttcct
     3481 ttatcaataa ctccacgcat aagatcggtc attatgaatg ccacatcttc agaaacaact
     3541 tgcatctgat tgttgcgttc ttttattgcc aaaagatcgc caatttccct ttcaatgttt
     3601 gctatttcgt taccgtcttt atccagaacc aacctgatac cgtgtggtat cacatcgcgc
     3661 cccttgtttg cataaatggc gtatgccccg gccatttcaa acggggttaa ctcagaagtt
     3721 ccaagagaca tcgttgggtt agagttaaat ctgaaaggag gtacttttaa aattctggac
     3781 gaaaactcag ttattttttc tggaccaacc atatcataga ttcgtacggc aataatgttt
     3841 attgattgag ccagtgcatg cttaatattt acaagacccg agtaggtgcc accataatta
     3901 ccaggagccc atgtagtgcc gtgagagtcg atgtttacaa gaggcgcatc tgatattgta
     3961 gttccgctgg ttataatatg ggaatcgata ccggcaccat agacaaaggg tttaaaggaa
     4021 gaacctggtt gtctacgggc agaaactgct ctgttaagct gattatctac attaaaatca
     4081 gatcccccga ccattgcgga tatataaccg gtggagggct cgattgatac tagagcgcct
     4141 tcaacctgca ggttagttac aactcctaaa gctgttgtac ttctgaagtt ttctataact
     4201 tcttgattta ttcgtgaatc ggtaagcaga gacaaaagat ctgccgcatc tgcaacttca
     4261 tcaagtatag cacgtctggt tcgtgattct atgtcgtgtt taataacaac tccaggaagg
     4321 ctaaaaatca ttctcaatag tccgtatgtg ccgaatagcc ctcggtcgac ggatccacgg
     4381 gtgtaggcgt ttaatgagtc agaaagcttg gtttgttctt ttagaccctt tttcatatga
     4441 tattctgcgg atcgctgtct tcgaagatca agagtcgtat atacaataag cccgtcattg
     4501 taaacggcat ctctgccaaa acggttttca agaacttgcc ggataaaggc tgtgaagtgg
     4561 ggagccttgt cggtattttt tgtataagcc gtttttgtcg gaaactctgt tttcagagaa
     4621 tcaataaaat cgggccagaa ctccttgtaa attgtatcgg cctgttcttt ggtaaggtag
     4681 ccttcctgta ccattcggtt caagatatca cggttctttt catacgcatt tcgcggattc
     4741 attaaagggg agtatcgtcc aggtgcagaa gggagagccg cgattactga tgattcagca
     4801 gcggtgaggt attttacctc tttatcaaaa aatagtcggg cagttgtggc gacaccatag
     4861 catccatgtc cgagataaat ctgattaaaa tacatttcca gaatttcttc ttttgaaaat
     4921 tttctttcga tttgaagagc aagtatagct tcaagggcct tgcgaccgta tgtcttttca
     4981 ctctcggtaa acaaccgctt cgccaactgt tgggttatag tggaaccacc ctgtacaatt
     5041 tccccggctt gaatgttttt gatcatcgcg cggatgattg caccaaagtt gattccaaaa
     5101 tggttataaa aagcctggtc ttcggtagcc agaagggcgt taatcagtgt ttgtggtatc
     5161 tcgtcaaaac caacgagctc ccgtttttcc ataaacagtt cggcgattaa ttctccgtta
     5221 acatcgtaaa gccgtgttgg cacagaaagc tgaaactgtt tgatattact tattccggtg
     5281 aaatttttaa tttcagacat aatatatccg aaaataagag cacctataat agaacctaac
     5341 attaataatg atatgataca ttttccgaaa atagtggtag aactttcttt ttccatttta
     5401 tttgtaatcc gtgttaaatt tcttttttaa aaattataaa attatttttt tatcatttca
     5461 ctaagtttcg cgattgagta tatgattgcc gcaacgacta gcgcgaaaat aagcacagta
     5521 attataatgc ttaagagcca tcctgttatt ttcaataaaa gtggaagaat atagcgaatt
     5581 gcaattatta tggttgcaag tacgccaatt gcataaaaaa attgggttaa tggtcctttc
     5641 ttcataattc cccacaggtt ttatagaata ttgttttatg agatatagac attattcgaa
     5701 aaatccggtt tatagtataa aatttctctt aatatataaa atacctaatt ttaaagaata
     5761 atttgttaga tatctgtcaa taataataca ggattagcca ttatcatgac attttgatac
     5821 gttgtttggt taattagggt ctaatttgcg atttcctgag ggcttattcg agaataataa
     5881 accttttgga gatttggttt tccagttttt tcaaaccatt acgtctattc cctattattc
     5941 tgcaggttta gaggttgttt caacataaaa attcattcgc ctgccgtttc tccatatact
     6001 tattttaact ttttctccgg gattggaatc ttcgataatt ttaacgaaat cgtctattgt
     6061 tgatattttt tttccatttg cagagtaaat tatatcaagt ggacgtatac cggctatatg
     6121 agcaggccct ccttgctgta cctgggctac gataattcca aatgagtgac tccagccaag
     6181 cttggaagcg tactcctttt ctattggagt gagagcaatg cctacaaatc ctttctgaac
     6241 gtatttttta tctttcagtt ctatcataag ctctttaatg ctattcacgg gtatggcaaa
     6301 accgattccc atatatccac cagattttga atagattaac ctgttgattc cgataacttc
     6361 tccatgaata tttatgagtg ggccacctga gttacctgga ttgattgcgg cgtcagtctg
     6421 aatgaaagag gagttatcac tcatgtctat gtttttgcgt ccagttgcac tcacaacccc
     6481 aactgtaaaa gattttgaga gaccgaaagg gtttccgatt gcgattgccc aatctccgac
     6541 cagaacagtg tctgaatcac caaaaaatac aggattaaga ggctctttgg gttctatttt
     6601 tagaattgtt atatcttttt caatactgaa tcctcttact tcagcttcgt atgtatcatt
     6661 ttcaataata acagtgattt tttcaaccac tctgttttgg ggagctatta cgtgaaagtt
     6721 tgtacatatg aaaccgtctt cagaaataat aaaacctgag ccaagaccgg tctgtttctg
     6781 aatacgtgga attccgaaca attcataaaa tgggtgactg gggagtgtta catggtcttc
     6841 agttgaaata aatacaattc tgttctcgta aagattgtaa attttttgaa atgatttttg
     6901 aatatcccat gcttttcgta tttcagagtc attgcgtaac ggcgatttgc ggtctaaccc
     6961 tattaatcca ttttccggat ctggttcttt acaggagaag tatataaccg ccgcaatcaa
     7021 tagtatcagt gcaacaggta ttgcaataag cttacgcttc atcattatcc cttgttttta
     7081 attgtttggt acagttggaa ttggctaata ttatagccaa gcataatcag aactttaaag
     7141 tctgattatg ctttttgatg atatcagggc gtttgaaagt ttctgtacaa agcggtcata
     7201 agaaattgct gatgctcttt tatgcataac tttacagtca atttgtgagt aaccgtttga
     7261 ttttagatcc tctccaacag caagaatatg ggaatcaagg taggagaaga gtgttctctc
     7321 caatggattt gtgcttgtat ctgttgtgat atttattatc ttccctttgc cctgtgcaac
     7381 catcaattct agaaaatatt taactgtaag taccgtgttt gttacgtaaa aattggttat
     7441 ttcctcaatt gaatccttgt tactgtttaa aaggggcata ctgccttctt caaaattttc
     7501 aaattcgggt ttgaagctta ttatcaaata gtcgggagta tcgccgtttt ttaaaattct
     7561 tgtaagattt ttaatgtatt tttcagtatc gttaaattga acatgatggg cgtacgacgt
     7621 agctcgcata ttaccgagtt cgcgatcaat actcgtaatt gcctcttttt tattgcttat
     7681 aaaaatggat ttgcaacgtt gcagcgcaaa aaaacgcgaa atgtgtttga aatactcata
     7741 attgtttcca agataataca catttttatt gattaagtgg tcgatagttt tcatttttta
     7801 agaactctcc aattttgtca ttttactaat ccgcgaacgc accatattca gatttgcgag
     7861 catgcagtct ctctttggtt atctggtcgc gaaatatata gtagtagttg tttgaattat
     7921 ccgcgtttat ctgatttatg ttgactcggt tttcatatga cttatttgaa cagccgtaaa
     7981 tcaaaaaaca gagcgagata aataacataa ttttgaaaat aattttcatg attttagtat
     8041 aaaaatcaat aatgaattat caaggatttt ttatttatat ttttggttat ttataaaaag
     8101 tgtgctttct gggttttaaa attataattt atgagttgtt tctgtttttt tgagagaaaa
     8161 ccacgactgc tttttttatt gctttttata ttattgttgt ttagttcttg ataaaaacag
     8221 taataatggc cttattgggt ctaacaatag ggaacttcga aaaattacaa tgttccagag
     8281 ggcagcatgg tttaggtgcc tattctaaag gaatttttcg tattacccgt gggattgttc
     8341 tgacaagaat aacctacaag ggaaagttcc ttgttgaaaa gttaataatt agaagttacc
     8401 aatagtaatc aggggtaagc aatgaaagtt gttcatgtct taaaacgtat tgaaacgatt
     8461 gatcaggaca tcaaagaatt gaagaaactg gaaaaatcga tcgcatcaaa taaatctttt
     8521 tcaacgccta ttttcatctc gattgagcag cagattaacc ttttgctgga cgaacgaata
     8581 aaactctttg gtttggagct tgctaatccc cctcaatctc tactggatgc tgttggagaa
     8641 gaagaggaag agttcaaacc tctattaaat accaaaacca ccaagaagag cgccaaaaaa
     8701 agcagtaaaa agtcttcatc tgcgcctaaa aagaacaaac agcaaccaaa ggaatctaca
     8761 gatgacgaag atgatgattt gccaatgagg atgttgactc aggagcagat tgatgctaaa
     8821 attgatatta tgtcttcagt cgaagaagaa aagccaggat tgggccttga caaggataag
     8881 ctcaacgatg attcggtgaa attgctcgat atcgcccttg acaagggacg gtctagcgac
     8941 gaaaatgttc tcccaaaaga aaaaaagagg gttcggtttt ttaaggataa ttttccgggc
     9001 ggccactact gagagtttcg ggtcgcgtca tatataataa gcctcacgat ataaaggttg
     9061 ccgatgaagg acgctacaat tatttcttat ccaggatatc atgatgacta tctgctttca
     9121 ctcacagggt tgcgctcaag atatatggtc cctttcgcgg ggcgctatcg tgtagtggat
     9181 tttacattac gtaatgcttt tttttgtaag gctaaaaatc tttttatttt tagccgtgta
     9241 aatgatgatc ttgctttcta tgttgaatct catccgattg tcaaaacaaa caaaacaacc
     9301 gttgttaaaa ccgttatatc cgaaaaaacc gaaatcagaa tgatttatga tatggtaaaa
     9361 agtacccaga cagcatatta tattcttcat tgcggtgaca accctggttt tatcaacttt
     9421 tacaagctgt ttgagaaata ccagacaaaa aaggcagcta caatactctt taaacttaaa
     9481 ttaggtgata aagcctctct tgcttatacg atattgatta caacgcgcaa gtttcttctt
     9541 tctgttttaa aaaaagcttt gaacagtaac gaaacctcgc caaatgtctt cgagatgatt
     9601 gtaaacatgc taattaataa aggggttttg aactcgactt ttaaagccac ttattggccg
     9661 attagatctg ttcctgatta ttatgattat aacatgtttc ttttcaagga tcaggcaagt
     9721 tttaaccgta tttacgaaga caatcttttg ttaagcggta tcgaacgtga caatacaact
     9781 cagataggcc cttttgccaa gataacccgt tcttatatct ctgatggatg catccttaat
     9841 ggaacaatag ataactcgat tatttttcca ggagtaattg ttgcggaaag agccgtggtt
     9901 cgtgattcta ttcttttgcc ctatgtcgga attgggcaac agagtgtgat tcagcgttct
     9961 ataattgatg aattcactga ttacaatcag agtgatatat tatttaatat ctcggaaaag
    10021 gtacgtatag gttctgacga attacagttg aaaaataatg attttcctcg ttccatatat
    10081 aatgctatca ctcttattgg taaaaatagt atcattcctt ctggagtaag agttggcgct
    10141 gcgtgttata tcgcgtcagg agtttcttcg tctgtctttg atgagaaaaa gactctttat
    10201 gacggacttt caattttgaa tagactggtt aaaaaggaat gatgggtgag gttaaaaaac
    10261 cgcgacgtta cactgcctct agctccgaag aaaaaatatc aaaatatatc gaagaaaata
    10321 gaaggcggga tggcttccat ctgaaaagta tgggcaaggt tttccctatt atgtttgtat
    10381 cgtatagtaa aaagaggctg gaattttctt cacctaaaca aatacactca aaaaaactta
    10441 gactttactg gaaaaatatc taccattgtg aacttgatac ggttgcaata gctgaaccac
    10501 gaccatcagg tgagcacttt atctatactg tcaagatcag acatctttta actccagaga
    10561 gccaacgttt cgagctacgc tataccgtac cagctgatgt ctataagata ggcaaaatca
    10621 ctatacctgt agttgaagaa acagcagttt cgatacttaa tagtgatgat tatcagaatt
    10681 gcaaaaaaga gatgctgcag aagcttgaat actatgactt tttcagaatt ttccccttcc
    10741 agagtgctgg agcaccaagc gaagttgttt tttctatgat ttcaaataca atactttacc
    10801 ttaaagatat ttctcaatat aataatttta ttgaaaaaaa ctcagcattc tttgacgttc
    10861 ataaatcttt gcgtagtcac ctgcttaaca ggctaaaaac tcttaatctg cgctaccact
    10921 cgcttttggt tagaccattt gattacgtga ctatagcagg ttcgcaattt accctagctt
    10981 attttgttgt tgcccgcagc agtgaagaga taactaatga tgacatctta tatcttgatc
    11041 gtatcgcgca tgatttttat caatcgcttc aggtttcatt gtataaagtg aataactgtc
    11101 aaggtagaat attgaacttg tctgtcacag gtgccagaat tttttttaaa cgtgaaatgg
    11161 taaactgtat aagtgtttca agtgagatga gtgttgtgtt tagtgtcgtt ggagtagccg
    11221 atatttcaat tgctggagaa gttgtttata tcgaacaaaa gaaaaacgga gtaattattg
    11281 gttttgtttt ccgtaaatca ccttttggaa gccgattggg gaagttcctg catgaaacat
    11341 ttcaaaatca tggactctca atctgttaat agccgttttt ttggagataa caatgggttt
    11401 tctgtcaaaa ataactagaa ttatgctgtt tgttttgttt tcttcggtag ctttggctga
    11461 tgagatcgcc gtgatgcctt atcttgtaga aactaccgaa gtttctgcaa gaaatagtct
    11521 ggatgatgaa tacgccggat tactcgggat tgccgcggca gttgaatacg ggctagactt
    11581 ttacagtatg gataacctga aaaaggatct taaaaatatt ggaattgatt atcacagcat
    11641 tttaactgcc gatgacctta gactgcttgc caagagaagg atgctcaaat atattgcaac
    11701 aggtacaatt tataagacat ccaacggtta cactgtaaaa actgttcttt atgatagtaa
    11761 aactgaccag atcgtagcca agagttctgt atccggccgt accctttttg acactgcaaa
    11821 aaaagattta catgatattt tcagatatat gccgactgcc aaaacgggtt caaaaaccgt
    11881 aatcgctgaa tcagttctat tacttgattg ttcttataat atgattaacg aaaaagttgc
    11941 cttgattgat tcactaaaaa agcattatga gacaatcttt cgacaatatc ccggtagcaa
    12001 agtatactgc ctcgctttta attctcaaat gaaatcagaa accaggagct tttttacatc
    12061 aggagcctta aatagttatc tggacaatat caagtttaat gcatctaata acgacaaatc
    12121 aattcgtttg gcactggata gtgctgtgtc aaacataaaa tttcagaacg gttccttgcg
    12181 ctatatcgta ttccccttca actctaatat gaaaaatatt tcatatttta cacgattcgc
    12241 aaatcaggtt gccgccagaa gggctactgt ttatcccgtg gtgggggcaa aggttgatat
    12301 ctcaaataat gctgccttaa catctcttgc cgagagaact ggaggaaatt tttatcctct
    12361 cttataccat cagacgtttt ttgaccgtaa agcagagagt tttcatcttt ttatgaggtc
    12421 cggacgtctt tatttctcgg aacttccata tccgcagtgg aattcagagt ttaaaagcgg
    12481 tgaagtgaaa cagcagtttg atgtcccaac ctggttaaac ccatacaacc tggaaaaata
    12541 ttatagagat ggccttaaaa gggttatcgt caagagtaat ccgcttgaag taaattcaag
    12601 atttgtattt gcggatatta ttgataggtt taataaaaag aatagtaggt ctggttacag
    12661 tgtaacaata gccagagtaa tgctcagttc tgtcaatggg tcggtatggg ttgatgtcaa
    12721 ggatcgtgaa tctttaggat atttttctcg gttaaaaaaa tcgggtgttt atagtttgat
    12781 tggagtccgg cttcaggttg atagtgactc tgtatatgga tacagtataa atccaaaggc
    12841 ttttgttttt tccgttcccc aggattctgt gccggagttg cttaaggttg atttgaacga
    12901 tttgcttggt agcccttctt catatacaga aaaagggctt cttgaaccac ctgtctggtt
    12961 tataaatgtc agggttcaaa tgatcgattt aaaaaccgat gttaaagaca taagatctta
    13021 aacaatatag aggttcttga taaagatttt ttaattaatg tcgacaaaaa catgtttatg
    13081 tgatatgatc atatttaatt gtatattttt ttatcgtaat tagttttaat tatcaaatgt
    13141 gattaattat atttaatgct ataatttgat aaaataacat tagttaagac gacagataaa
    13201 acaaaatgat aatttgaaat ctttaataaa cgagatttat actgcatttt ttgggagcgt
    13261 tatgaggatt tttgctggtt ttataataat attgatattc atgcctgttt ttgctcaaag
    13321 caggcagtcg gttgtgatta cagattttta taatacatca cgtattaaaa cttcggatca
    13381 ctattccacg attgtacctg ataatattag aaccgaatta gatcgaaccg gttattattc
    13441 tgttaaacgt acagtcgcga ggaatattga attagatgaa gatggtatta aaaaagctgc
    13501 tgttgaatca aatgctgatt actttattac cggtgaatac acttccagag gtggagaaat
    13561 tggtgtcagt gcgcatgtgt acgtaaaaaa tatcgataaa gtagttacga tagtgatgga
    13621 gcctaaaaga gttggtgttt tccttaatga aataatagat aacatttcct taaaaatagg
    13681 tttcgagttg cagaagtatc gtgataggtc tggagagatg ccggttattg aaccagcatc
    13741 ctctgatttt agttattatg ccgaaattat tatcagttcg ccctttccag acatgccggt
    13801 ttattatacc cttgacggaa ctcttcccgg gccaggaaac gggtttcgct atagtaatcc
    13861 ttttactgtt tacaatgaaa ctgttgtgaa ggctgtctcg attgataaag agggagttcc
    13921 gtcggatgtt gctgttaaga atttccgccc gctttttgtc ccttctgttt ttgattttaa
    13981 gattctttat ggagttatgc agtttgttag ttctggtgat gatgttgaat cgccaattga
    14041 cacaaccatt atttcagtag ttccggtttt ttaccttgga gggttcgaca catttaagaa
    14101 gacagctgtt gcacgaaatt tgggttttgg tggatggttt gattttgcaa atgctgacct
    14161 tgatggtggt tatcaataca agatacaggg atacagcggt ggtgtttttc ttcggtttaa
    14221 tccctttaat aacttgtctt tagagatccc ttttactgta ggcaagatgc ggacttctct
    14281 aattgaagaa atgtcagttt atgaaaatat tttttcagag ccaggtgata cacttgcaag
    14341 tgatcttgag acatattgta atagtggcat agttgctacg tatcgagtaa gcaattttca
    14401 atttattgca tcagctacaa tgaaatatat tctttccgac gacgacccta caaaaattct
    14461 tgtgtatcag gcaggtgctg gtgtctgcta ctgatttatt attgtttgat tgtggaaata
    14521 gtacaattaa ttccgcaata agccgtaatg gtgagtttcc ggataatttt tttacattta
    14581 accataaaga taacgatatt gaagatcaac ttgtctctta tatccgcaaa aatggaatat
    14641 ttagagctgt ttttgtgtct gttgttccca tgctaacaca aaccttgcta tctgtatttg
    14701 ataaagaagg tgtagcttgt atcgaatttt atgcggatca atgtggggtt tcttccttat
    14761 atactaatcc aggtagtgac cgaatactct tcgcctattc aataaaaacc ttttacccag
    14821 ggcgagctat gtgtctaatt gatgctggaa cggctttaac ggttgacggt cttgatagaa
    14881 gtggaagatt tttaggtggt tttattatgc cgggagtaaa aacagctttt acatctattg
    14941 ctagaggtgc cgcacttctt tcggggtatg agctatcaat ctcgcattct gttggaatag
    15001 ataccgaatc aagttttggt tccggtttat ctgttttata ttccggaggt ctaaaggcgg
    15061 caatagaaaa aatatcggca ttgtgcggag ataaggtgga tgttgttttg acgggtgggg
    15121 atggaccttt ggttaaagag cttttgtctg attgtttgct tgtttatgat ccacttattc
    15181 ttctaaaggg acttgttgtt tattactata attgttcgca ttgatctttt gaaaaggtat
    15241 taaccaaaaa aagacttgca atatagccgg cttatacaat agtagtattg taatgattaa
    15301 aagagtgggt ttccgctctt tttttatttg ggagactgcg tttctgttgg tcgcgttgct
    15361 acccttttgc caggtttaag atttgaatca agtaaaaaaa gacctcttca tgtcacttgc
    15421 cgaacagacc gcactccgca gtggaattga gatttatgat attgttgtgc agtttcgcaa
    15481 gggagtagtc agagtcgata ttcgtatcga taatggtgcg actgtttctc ataatgactg
    15541 ttctcttttt tgtaccgact atattgaagt tttaaaagag catggtcttg aaaacgaggc
    15601 tgccgtagag gtctcctctc ccggtttaaa acgtaaaata cgcaatcata aagagtggct
    15661 tcgatttgaa ggcgcacctg tgaaagttac ttattctgac gagaaagggt cggtaacagt
    15721 aaaaggggtt gcaaaagagg tccttaagga ttcggtggtt gttgtagaaa ataaaaaaaa
    15781 gcatgttata ggttatgata ctatccggca tgccaatctt gattactgaa ggtcatagaa
    15841 aagatgaatg taaacttttt tgaggcgttg catcaaattg caaccgaaaa agggatttcc
    15901 ggagacgaat tggagcagat tgttgaagca tcaatggttt ctgcctataa aaaacagttc
    15961 ggtacaaccg ataatatgcg ggttgttttt gatcgcgata aaaaaaccat agcgatagcc
    16021 tccttgaagc ttgttgttcg caaacctcaa aatccaggtg aagaggttgg tcttgatgtg
    16081 gcacgagagg ttaaaccgga tattcagatc ggtgatagca ttgagatgaa tgatgatcct
    16141 ttcaaacttt ttggtcgaat tgccacacag actgccaaac aggtgatcat acaaagaata
    16201 aaagaagctg agaaaaatat tatatttgtt gagtttaaag cgaaagaaaa acagctgatt
    16261 aatggtttta tgcaaaggcg ttccaacaat actatctttg ttgatattgg acatggtggt
    16321 gtagaaggta tattgccacg aagagagcaa tctccacttg agttttataa ggaaggggat
    16381 cgaattaaag cgcttgtttt aaatgtcgaa aataagacta aaggtccaaa tattattctc
    16441 tctcgcgttg ccccagattt tataaaagag ctgtttaaaa tggaaattcc ggaagtatat
    16501 gatggaattg ttgaaataaa aagggtcgtt cgtgatccgg gaatgcgtac caaggtagct
    16561 gtaaccagct atgagcgtga cattgatgct gttggtgcct gcgtgggaat gaagggaagt
    16621 cctattcagg caatcgttcg cgaacttgaa ggcgaaaaaa ttgatataat tgagtattct
    16681 acagaaaaaa gggttttagc tgaaaatgct ttgactccag ctcgcatcaa tgaaatttat
    16741 gaaactcgag atggtggttt aatagctgtt gttacttctg atcagtataa tcttgctgta
    16801 ggtcgtgcgg gtcataatgt gcggttggca tcacagctct gcggcgtaga ggttcgcgtt
    16861 aaaactgagg agcagtatag ggaacttgtc gagtctgatg attctatgct tatgtcgaat
    16921 cagcttttct cagaaccggt tgaggatgaa acttctctca ctgaacttga tcttgattct
    16981 cgtacaattg agcttctcga aaaaggtggt attttttctg tcgaagagct tgttgaaagc
    17041 acaattgatg atataaaaaa tattgatggt ataggcgaaa aaaccgcacg tcgaattttt
    17101 gagattttgg cagaagttgt tgattttgaa gatgaagaag atgaagaaga tgaagaagaa
    17161 tagaagttgg caaaacataa taaaaaagta attgtactta accggttgtt ttagacggtt
    17221 gtataaaata tgagttttaa aagtacccat aaggaattaa atgaaagcag aagaaattgc
    17281 aaagaagaat ttggtaaaaa ctgaagatgt gttcagcatc tgtaaagact taggtattga
    17341 gtgtaaagat gccgagagta gtctctcgga tagtgatacc tttctgattg aaaagaaaat
    17401 tgagactatt atagccgaaa agaaagctaa aactcaaaag aaagcgtcta ccggtgttaa
    17461 aaaaaagatt aaattgaaaa gaaaagtttc ggcagatgcg gaatctcatg taatttctga
    17521 aataaaaaaa cattccgatg aaactgctcc agaaaaaaca ggagctgata aaacttctca
    17581 accaaagagc gctgtaaaaa aatcttcaga gcgacctgct gataagtcta gtgcatctgc
    17641 gtctcgtcca gttcaggggc gacctgcagc ttcttcttca tcaaggagta atacagcacg
    17701 tccacaaggt tctgattcaa gacgtcctgc ttctggaaac cgtccgacac agggtactcg
    17761 tcctgcgcaa ggtacacgtc ctgggcaagc tgcacgtccg acacagggtg cacgtccggc
    17821 tcagggagcc cgtcccggac aatcttcaac cggacagcgt cgcccgacag gtgcgggagc
    17881 tgcacctttt actaaatcca cacaagaagt tcctgtagca tcagaagttg gcgataagtc
    17941 tggcaagtca aaaaggcgta atcgtgaacg tgataaagaa cgagaaaatc ttaagaataa
    18001 acgaagtgaa aaacttgatc gcgaacagcg agtatataat agccacatta caaaaaaacg
    18061 aaaacctgtt gtaaaacagg tgcgtgaagc agttactccg gatagtattg aaataactga
    18121 aaatatttct gttggcgacc tagccaaaaa acttaatgtt aaagcaaacg aagtaattgg
    18181 aaaactgatg aaattaggcg ttcttgccac aattaatcag atccttgact ccgaaactgc
    18241 agaaatttta gctgaggagt acggaacttc tgtcaaagta gtatcattat acgatgaaac
    18301 cgtaataaaa gaagatgaaa tacaaaagga tatagatgtt attcaacgac cgcctattgt
    18361 tactgttatg ggacatgtcg atcatggtaa aaccaagctt cttgatacaa taagaaaatc
    18421 aaacgttaca gctggtgagc atggaggcat aacgcagcat attggagcct ataaagtaaa
    18481 agtacatgat cagtgggtaa catttcttga tacgccaggc catgccgcct tcacaaccat
    18541 gcgagcaagg ggagcgcgtg taaccgatat tgtaatatta atagtagccg ctaatgatgg
    18601 cgttatgcct caaacagtag aggccataaa tcacgctaaa gaggcaaatg ttcctataat
    18661 tgttgcgatt aacaagattg atcttcctga agctaatcct gcgaaggtta ggcaagagct
    18721 tgccgggtat gatctcgtgc ccgaggagtg gggggggaca acactctatt gcgaaataag
    18781 tgctaaaaat gacataaatg taaacgcatt gcttgagcag gttcttatgc aggccgaaat
    18841 tcttgaactt aaggctaatc caagattgag ggcaaagggt tctgtaattg aagctaagct
    18901 tgatccgggt cgtggtgctg ttgctactct tcttgtagag acaggcacat tgcatgttgg
    18961 agactcattc gttgttggta tatatgctgg taaaataaga gccatgtttg atgatcaggg
    19021 gaactccgtt gctgaagcgg gacctgctat gcctgtcgag atattgggac ttgatggtat
    19081 accatcagca ggtgatccgt tccaggttgt cgcctctgaa aagtatgcca aacagattgc
    19141 gggcaagaga caggagttgc gacgtgttga atctgcaaaa caggttaaaa aggtatctct
    19201 tgaagatctt aacgatatga ttaaggaagg agaggtacag gatgtacgtc tcattattaa
    19261 agcagatgtt gacggttccg ttcaggctct taaagatgcg cttgaaaaac tgtctactga
    19321 tgaagtacgt gttaaggtga tacatgctgc agcaggtgga atcaacgagt cagatgttat
    19381 gctggcttcc gcctcaaatg ctattgttat aggatatcat gtcagaccta ctccaaaagt
    19441 atctgatctg gcagatcagg aaaaagtttc gataaagacc tacaatatta tttttgaagt
    19501 tactcaagac atcaaaagcg ctatggaagg catgcttaaa ccggatatcg aagaagaatc
    19561 tactggtatg gccgaagtca tgcaggtttt caagatttca aaattgggaa caattgctgg
    19621 atgtacagtg attaagggta aggttcatag aacgaacaaa gttcgccttt tgcgtgacgg
    19681 cgttgttctt tttgatggag atttgaaatc gcttaaacgc tttaaggatg atgcaaatga
    19741 agttgaaatg ggacaggagt gcggtatagg gctaaataaa tttaatgata taaaagttgg
    19801 tgatattttt gaatcttaca ggcttgtcga aatagctaaa aagttgggtg attaaattgt
    19861 catcataccg aaaagaaaag cttgaagagt tgataaaaag gttagttgca ggtcttttat
    19921 taaaagagat aaaagatagt cgaattgggt ttatttccat ttcaagagta gaaatttcaa
    19981 aggattacag tagtgctaat gtgttttttt cggttttagg tagtgaaaaa aacattaaag
    20041 actcattttt tggtcttgag tcggctaaag ggtttatacg caaaaaaatt ggtaagagtc
    20101 tgaaattaag gcatattcct gatttaaaat ttgtttatga tgattccgta gcggaaggag
    20161 taaatctggt aaaccttatt gacaaggtaa acgctcccaa agcagatagt ccggatgaat
    20221 aaaacggctt tcaatgataa agttatactt atcgacaaac agaaaggttt aacttctttt
    20281 gatacaatat ctcggctaaa aaaaaaagtc gggataaaaa aaatcggtca ttcagggact
    20341 cttgatcgtt tggcatccgg tcttctggta gttgcgacag gaaaagccac aaaattaacc
    20401 agatattttc tggattctga taagacttac aatgcggtag ttcagctcgg tatagttacc
    20461 gatactcttg atgccgatgg aactgttgtt agttctgcca gtgtcactac tgagcatatg
    20521 gcgggggtat gtgccgttct cgattcattt gtcgggaaaa tcgaacaggt acctccacta
    20581 tattccgcgt tgaaaataga tgggaaaaga gtgtccgatc gcgtacgcga cggtgaagct
    20641 ctaactttgg ggtcaagatc tgtaaatgtg tattccatag atctaataag tactgatccc
    20701 gtcagaagca cttttgaaat gagggtgcag tgctcaaaag gaacttatat tcgatcttta
    20761 gcgcgtgata tcgggcaaaa gctagggtgc ggagcttcca tactggatct tcgaagaaca
    20821 gcctccggtg aattttcggt agagcaagca gtttctgtgg agaatgtctc ggccgatgct
    20881 gatgagcgga attatattgt aacaattgag tctgcgttaa aattcatgaa tcaggttaca
    20941 gtcgataaga gtgctgtaac acgtattatg aatggagcct ggtttgacga gggatcgctt
    21001 ctgtctgttg ataaccgtgg gtcggattac tgcgcggtat ctgacggtaa aatggttctt
    21061 gcaatagcaa agatcaattt taacgattgg cacgttgttt atgaaaacgt gtttaactaa
    21121 aaaggagcgc tttgtcgggt aattgtaaat gtgttatccg ggtttgcgtt gtccctgtat
    21181 atgttttttc tctgaatatc gcgtttagcg tatgtggggt tttgatatat acaaaaattg
    21241 ggcggttaaa aaaattataa ctgctatgat aaaattatct ataactattt ggagaaaaca
    21301 aaatgaaaga aaaaaaagaa gtaatcacac cttataaaag acatgattct gacaccggtt
    21361 ctccggaggt tcaaattgct cttttaactg aaagaattaa tcatcttaca gaacatttta
    21421 aagtacataa aaaggatttt cattccagaa gaggcttact gaaaatggtc ggtcagcgca
    21481 gaaggttatt ggattacctt aaaaagaaag atctcggcaa atacagagag ctgattgcat
    21541 cactaggtat tagaaagtaa ttttgccgaa tacaaggagt tacacaatgt ctcatgatta
    21601 tgaagttgaa gtaggcggca aaaaaattat atttagtact ggtgaaatgg caaaacaagc
    21661 gcatggttca gttactgtgc gtcttggtga taccatgata cttgctacag cagttgctgc
    21721 tccaagcgca agtgagggac gtgatttttt cccactgcaa attgaataca aagaaaaata
    21781 ttatgcttcg ggtagaattc ccggtggata tgtgaaaaga gaggggcgtc ctggggacca
    21841 tgaagtattg atctgtcgta ttactgaccg ccctttacgc ccactattcc ccaaagattt
    21901 cttgaacgaa gtgcaaatag ccattaccgt tatttcatca gataaaaagg agttggccga
    21961 tgtacttgca ataaatggag cttcggctgc tctttcaata tcaggtattc ctttccatgg
    22021 tccggttggt gctgtgcgta taggacgcat agatggaaat tttattgtaa atcccactgt
    22081 ggttcaaaca gaggagagcg atattgatct aatggttgcc gggacagagc gtgcggttac
    22141 catgattgaa ggatcgtcta aaaatatttc agaagccgat atgctagctg ccgttgaatg
    22201 tgcacatgaa aatatcaaaa ttatctgtgc ggcacagaaa cagcttatgg aaaaacatgc
    22261 taagccgttt atggaatata agccgaaaga aaaaaacaac gaattagcat cgatagttag
    22321 agagaagtat tacgctgaag ttgaagcatt gatgaatatc cacatgaaaa aagagcgtga
    22381 agctgcctat aacgcaatag ttgaaaaggc aaaggcagaa cttgaagagc gtttttgtga
    22441 tgatattggt gaagttgaac ctattattca tgatatggat aaagatgtgg tacgtcaggc
    22501 catacttgat ggaaaaagaa ctgatggcag atctcatgat cagatacgcc cgattactat
    22561 aagaaccagt gttcttcccg gagctcacgg ttctgccctt tttacacgtg gtgaaactca
    22621 aagtctgggt actgtaactt tgggtaccga aaaagattca atgcgtattg attcaatgaa
    22681 cggtgatgca aaccgttatt tctttttaca ttataacttt cctccatatt ctgtgggaga
    22741 gtgtggacgt ttcggtggta ccggacgacg tgagatcggt catggaatgt tggccgaacg
    22801 agcattaact tatgctattc ctgatattaa ggattttcct tatactgtta gagtagtatc
    22861 tgaaattctt gaatctaatg gatcatcatc tatggcatct atctgctcag gttcactagc
    22921 tctttatgat gcgggtgtaa aaacgaagtc accgattgcg ggtattgcaa tgggacttgt
    22981 tcttgaaggt gatcgccatg ctgtcttgtc tgatattcaa gggttggaag atcatcttgg
    23041 agatatggat ttcaaggttg ctggaacgga agatggtatt accgcgtttc agctggatat
    23101 caagattgaa ggtataacat ctgaaattat gcgagaagca cttgagcaag ctcgtaaagg
    23161 acgtcttcat atcctctccg agatgagtaa ggccctgcct gtacctcgcg aagagctaaa
    23221 accgaatgct ccacgtatca gtgagattaa gatccctact gacaaaatag gggagctaat
    23281 tggtactggt ggtaaaaaca tcaagggtat catagaagag acaggttcgg aaatcaatgt
    23341 gagtgaggat ggcacagtta gaatatattc ttcagatcag agtatactta atgccacaaa
    23401 agaacttgtt gagcgttcta ttgccgaagt cgaaaaaatg aagatttatg agggtactgt
    23461 taagcgtata gccgattttg gagcttttgt tgaaattttg cccggtaaag aggggttgtt
    23521 gcatatatcc aaaattgacc acaaacgaat caagcaagtt accgatgttt tacaagttgg
    23581 tgacaaggtc aaggtaaagg ttatgaatgt tgacaaaggt ggccgtatgg acctaagccg
    23641 taaggatgca cttccaccgg aatcggagtg atgttatcag tacgatgaaa agatatactc
    23701 ttgcgaacgg tcttgttgta attatgcagc ccatagagca tgcagtgtcg gcttcagttg
    23761 gtctttgggt taaaactgga tcgcgagatg aattgcctga acagtatgga tatgctcact
    23821 ttgttgagca tatgctgttt aagggaactc cccgatatac cgcaaaagag ttggctcagc
    23881 ttgttgatcg catcggcggt cagcataatg cggccacaaa taaagaatat acctgctatt
    23941 atttgaatgt agtatcagat tattgtgtaa ccgctgtaga tgttcttgct gatatgtttt
    24001 ataaccccct ttttattgac gcagaaatcg aaaaagagaa gggtgttgta attgaagaga
    24061 ttggcatgta cgaagacact ccggatgagc atattcacga tctttttact tccgtaatgt
    24121 ttaacggaca ccctttagct catctaatat tgggaaccaa tgagagtgtt tcctcaattt
    24181 cacgcaatac tataacctca ttttatgatt cttgttacgg tactggcaat agcgtacttg
    24241 ttgttgcagg ttgcttcgat ttggaagaga ttactttgaa aattgaagag tgttttgcgc
    24301 aaaaaagagg atatgaaaag aatattgacc tctcttctgt gtattctcaa atgaatactg
    24361 agcggatata ccggcaccat gtggataggg aacttgaaca ggtccatttt tgtctaggtg
    24421 ttgacgggta taaaaaacag gttgcagata gatgggctct ttatcttgtg agtacaatac
    24481 ttggtgggag catgtcttct cgtctcttcc agagggtacg cgaagatgaa ggactctgtt
    24541 attccgttta tagttttcat tcttcttata gagataccgg agcttttggt gtatattgtg
    24601 gcacatcttc tgaaaagtat gaaagggcac ttaaacttat acttgacgaa ctggatatta
    24661 ttgttaataa gggtataagc gagagtgaac tggatgatgc aaaaagattt atgaaaggta
    24721 atcttgcact ttcacttgaa agcattgaag ttcgaatggg gcaacttgct cgggatgaga
    24781 tacattacgg agcttttagc tcttatgatg atatagtaaa aaatattttt tctgttacaa
    24841 cggctgattt tgacagagtt gtgcgggatc tttttaataa taagcgaatt tcactggttt
    24901 caatcggaaa taaagtaaag tacgacgaca ggccgcttta tatcggtcaa taatgaggaa
    24961 taatggcacc aagaattgac agtttaaaca gtacaatagg cgatggatcg gtttttgaag
    25021 gtaaatttta tataaatgga tctttaaaaa ttgatggaaa gtttgagggt gaaattaaga
    25081 ctgatgagga acttgttgtt ggtgaaaccg gcaaggttag gacaaatata gaagccaaat
    25141 cggttactgt tgccggtaca gttataggca atatcaaagc acttaatgag gttagacttc
    25201 ttgaaacagc aaaggttctt ggtgatatag aggctcctaa tgtttctgtg cagaaaggtg
    25261 ttgtattaac aggacgtata aatatttgtg gtgaatataa aaaagatttg gaaaaagtcg
    25321 tggaagattc atataactcg actcctttgg aaaaatataa aaaacagtaa taataaggcg
    25381 tgatatagat cggatattgc acttttttcc aaccgaatgg acggatttta aaaaaagtgt
    25441 gcaaattttg aagatgtgcc gatatatacg gtatacggta taaatatatg cagaaattta
    25501 caatacgaaa gctctctgtt tcccgaatcc ataatgagat tatgtttgtt tatcgtggaa
    25561 aggaaaacat atatgttaag aagtttacat ttagtgtaaa tcgtctgtat caccgtttgc
    25621 tctattttgt tggagcttca gttttgacta ttgccttggt aattgctctg cctttcggag
    25681 gaaagaacgc cgattcgagt acaccagaag atgaagaact taaaaatgat attcttcttt
    25741 ccgatgagac tgattttaca gaacaggata aacgtaagcc attggctgta cagatttata
    25801 aagtgcaaca gggagatact ctaagtgaaa tagcggcaaa gtttggagta tcaactgata
    25861 caatctgcgg atcgagtggg cttacttctt atgattttct aaaaataggt caggttttaa
    25921 agatcccaga tcgagacggt atgcttgtaa ccgttaaaga aggcgaaaag ctaatagaca
    25981 ttgcccaaaa gtacaagatt cctgttgaaa atattatggc tcataatcag ctattaaacc
    26041 ctgacttttt gccagtaggg tatgatattt ttcttcccga tgctaagccg caaaatataa
    26101 taaagggttt tatgtggcct acattctcac gtagaattac ttcggcgtat ggttggcgaa
    26161 ttcatccgat aagtggtcga cgtcagtttc atcatggagt tgatattcgt tcgcgttatc
    26221 aagatataaa agcagctaaa tatggcaaag taactttcgc tggctcaatg ggagggtatg
    26281 gtcgtgcaat cattatagct cacccaggca atcagaagac attatatgcg catttgtcaa
    26341 aattatatgt taaatctggc caatatgtac gccaaggaca aatgattgcg cgtagtggta
    26401 actccggtta ttcagctggg cctcacctcc attttgaaat aattgaaaat ggtaaacata
    26461 taaatccgag acgtcatctc aagtaatctt tgcttgtgac tgtttataat tgcgtgtcat
    26521 aagaaaaaaa tacttgcttc aattaacctc tcctttaaag aatctaaaaa caaagcagtt
    26581 atgctatttt cttatctaat tatatttttc cggagataaa aaatgtggaa tcgtattaat
    26641 ttcttactcg agataggttc ggaagaaatt cctgccggtt atatatcgcc tgcctgccaa
    26701 gtgtacagcg atttgtttga aaaaaagttt aatgatgctt caataaaatt tggtgcgatt
    26761 catacatttg ctactccaag aagaatggtt atagccattg aagagctagc agataagcag
    26821 ttaagcgaaa ctgttgaggt gcgtgggccg gccgtagaaa aggcttttga tgataacggt
    26881 aaacctacaa aagccttgga aggtttctta aaagctagcg gtgcgacaat tgatgatatt
    26941 gacaaacgtg ataccggtaa aggtatctat gtgtttgtaa ataaaacaac tcaggctgga
    27001 aagactgttg atttattgcc gtcaatgatt gaagaaatta ttggttctgc aaattttcca
    27061 aaaaagatga aatggagcaa taagtcaata tcttaccctc gacctttgcg atacctgctt
    27121 acactattta atgataaatg tgttgaatac tcaattgacg gtataaattc taataataag
    27181 acccgtggac actatattca gcataatgga atgatagagg ttgcatcgat agctggatat
    27241 cctgatctgt tgaagaaaaa cggagttatt cttgatcagc aagaacgtaa acagatcata
    27301 aaaaatgctt tagtagacac agcgaaatct ttggatgctg tgctgataga agatgaagaa
    27361 ctcttcgaga cagtaacata tttagttgaa gatgtgaata tagttgtttg ttcttttgat
    27421 aaagaatttt tacaaattcc agatatagtt ttgatagcag agatgaaaga acatcaaaag
    27481 tattttgctt taagacagaa agatggatct ttgctcaacc gattttgtgt tgtaagtaat
    27541 aatcccgcaa cggattttgt taaagaaggt aatgagcgtg taattgcagc ccgatttagt
    27601 gatgctgaat ttttttacaa agaagaccgt aagcataaac ttgcagacaa ggtagatatg
    27661 ttaaagagtg ttctgtttca taaagacctc ggttctgtat atgataaggt tgaacgtgtt
    27721 agagctgttt ctcgagagtt tgttaagcga ttgaaaattg acaataatat agccggtaag
    27781 attgataggg cggttacgct ttgtaaagca gatcttaata cctctatggt tttcgaattt
    27841 acctctctac agggtaaaat tggagctatt tatgccttgt tggatggtga agattttgat
    27901 gtggcttctg cgattgataa tcattacagg cctcgctttc aaggggatga aaaaccttct
    27961 gatataatat cgtgtgtgtt atctctttcc gagaaagttg ataatctttt tggtgcctgg
    28021 tcggttggta atgtgcctaa aggatcgcag gatccatatg cattgcgccg acaggcttat
    28081 gctatagtag atcttttgat ttacaataag ataaatattg atttaaataa tctttttgaa
    28141 gtggttgctt taaattataa agatggcgcc cagttaattg agccaatact ggattttatt
    28201 tctgtaagag ccaaaactcg ttttcaggaa gataacattc agtatgatga aatagacgct
    28261 gttttaacta caggctcgcg ggattattat gaattgtata atagagcagc cagtattcat
    28321 gaatttcgtt ccagaaatga tttttcaaag atgcttgcca gtttcaaaag aatgaataat
    28381 attattacgt catatttaaa aaagaacaag gattatcaat ttggtttcga cagaaataaa
    28441 tgttcgacaa gacaagagac agcgttagtt gattattttc tggataagaa agatgttata
    28501 tcagatttat cttcaaaaca tgagtatact aagatatttg aattactgat aggtgctgcg
    28561 ggaattattg atgatttttt tgataacgtc atggtaattg atgaagatcc ggtaaaaagg
    28621 gatttgcgac ttggcttatt aaacgaaatt ttaatgctat tttctgcatt tattgatttt
    28681 tcgcgaatat cagattaatt ctgtgacaac agataataat ttaaatggta gagttcatgc
    28741 ttatgtaggt aatggcaagg gtaaaactac tgccgcctgt ggtttaatga tgagaactgc
    28801 cggttatggc aaattagctg tgatggtaca gtttttgaaa aaaagacaaa ccggtgagtc
    28861 catgtttgcc ggctggacaa ataatatagt tatacgtcag tttggaactg aagattttta
    28921 tcggccaggt aagtctgata tattgcttta cagaagatta tgtaggcaag ggcttgattt
    28981 cgctattgag aaggctgtag atgcttcttt gatagttctt gatgaggtgt tagatgctgt
    29041 cgattacggg cttattgtga tagatgatct tttttattta cttcatgatt gtaccttaaa
    29101 tgggacagag attattctta ccggacgtaa ggttgacgat acattggcaa atcattgtga
    29161 tttaataact gaaatgacaa gtgtgcgtca ctactatgat aagggtataa gtgctcgaga
    29221 gggcgtggag ttctaatgcg gctgcttttg gttgaaaatg agtttagtga gttgtcttca
    29281 ttcttgaata cgcatggtta tggggttgag aggacttctg cttcttcatt ttttgatatg
    29341 cttaaggtga gacaagattt taactttatt gtattgtaca tggaggaatg tgataaggag
    29401 actgcttcat taattatctc ttattgtttg aataattcat tatgctttgt tgttgtctct
    29461 tctgttttca cagatgaatt agcttcatgg ttaaaggaac atggtgtagc caggtttgta
    29521 actgttgacg aaattttcag gaattcgcaa atttttcgtt ttgttttggc atacgatgat
    29581 attgagcagt taagaatagt acatcttggt tctgatcttg ccagtcaccg gataatagaa
    29641 tctataagca atagttatgg agtttattac ctttgggtaa atgacgagaa gtcactaatc
    29701 gataatgtag attgttctac tgatatggtt ttattggata ttggctgtgt ggattttgac
    29761 acacagcatt ttttgcacaa ggctttgtat aatcgtgatt ttcttaagac aatatgtgta
    29821 ccattctctt ttaacggaga tatatcaatt actgatatca actgcggttt aaacaggtta
    29881 gccaaagttt tccttaatct tgatgagttg ttgggatttc ttgcaagata ttatcatgaa
    29941 cgagaacttg tttgcgctat gcatgcactg aaagagtttg tacagaagta ttgcgaagat
    30001 ggttgtctgt caaagagttc tcttcaagtt tatcatgaaa agggtgattc ttttttcgaa
    30061 cattttttgc cagatgatga ctattttgaa ggtatgcgcg aacgatcgca acgcttgaac
    30121 gccgttggtt ataaatccag catgtttaga tggcttcttt caggttggag ccgtggaaaa
    30181 aacggatctc aaaatgttta tatgcagatt cgtgattagt tgaacgataa gtatgatttt
    30241 tcttgacgtt gccggttcgc aggattacaa taataattat agtataaatt ggttttaaaa
    30301 ggaatatatt ttgaaggttg gtgagttctt acttgaaaat ggtctgataa attctgatca
    30361 acttgatgag gcgttgcttc ttcagaaaga taaccctgac cgggttatcg gtgagattct
    30421 ggttactatg ggtgtttttt cgaaagaaga gctgataatg gctatggaga tgtatatggt
    30481 cagtaccggt tttgatctca attttattga tgagtggctt gatcaagacg agattgatct
    30541 gctttcggaa aagattgata aacagaaaat gtaaaaaaaa aaagattaca gctataaaaa
    30601 ataaagttat tgacagatag agtgcgattt ctgatagtta ttcgcatgat agaatatgaa
    30661 gataagacct gctttggctg tggcgaggga aacccgcgtg gcctgcaatt aaaattaaaa
    30721 tatgatcctg ataccaagac tgcttatggt gagtatactg ctggtcagga tcttgaaggc
    30781 cctccaaata ttattcatgc cggtattcta gcggctattc ttgatgagac catgattatg
    30841 gtgaacaaga atatggaagt tgttgcatta acaggagaaa taactgttcg ctatttacag
    30901 ccagctttca tcgaggagaa tctttatatt cgaggatggc acgtgaaaaa gaataaacgt
    30961 gttatcgaaa atcgtgctga aattgaaaat gagatgggaa agattgtggc acgagctaaa
    31021 ggcaagtatc ttgaggtcga cgaaatacct cagcctgaat aaattggccg aagtggtgga
    31081 attggtagac gcagcggact caaaatccgc cggaggcaac tctgtggggg ttcaagtccc
    31141 cccttcggca atagtgatct taaaacaaaa aagccgtctt gcagacggtt tttttatgtt
    31201 taaataaagt ctttaaaagg tgcttgcata tatacgtaac tattatgttt tgtggttatc
    31261 ttaacagaag tgtttaagtg aataaattaa atatttgagg tatctatgtc agtatcgtct
    31321 tcagataatc agaaaaaatt tgatgaattt tttcaaagca tggagcagga tatttttgca
    31381 aatgcgaata gtgtacaaaa agacgagggt aaacctgtaa aggaaaccga atttagcttt
    31441 gatttaaaac cagaagagct agagacgtat cttaataaat atgttgttgg acaacaaaat
    31501 tccattgaag ttattgctac aaaggtatgt actcatttta acaggatgaa actcgaaaaa
    31561 gaactttcgg acgatgaaag ggttacaggc tcgatcaaga gtaatatttt gctgattgga
    31621 cctaccggag tcggtaaaac atatataata aaactcatag cggataagat aggtgttcct
    31681 tttgtaaagg ccgatgcgac taaattcagt gaaactggtt atgttggtgg tgatgtagag
    31741 gatcttgtgc gtgaacttgt tcatgaagca gatgaggata tttccaaagc ggaatacgga
    31801 ataatatatc ttgatgagat tgataaaatt gcttcttctt cgagtgggaa tggaattgat
    31861 gtttcgaggt ctggtgttca gcgcaatctt ttgaaattaa tggaagaggc tgatgtcgat
    31921 ttaaaaactc ctcacgatct tgcatcacag gtagaagccg ctatggaggc acagcgaaca
    31981 ggacatgtta agagaaaaaa aattaatacg cgcaatattc tttttatatg ttcgggagct
    32041 ttttcaggat tacaggacat aattaaaaaa agaatgaacc ttcaggctat aggttttgat
    32101 agcggaaata gtgataagaa agcattttta gaaacggaaa tactggctga aacacgaact
    32161 gaagatcttc ttgagtacgg gttcgagtca gaatttattg ggcgtttgcc tgtaaccgtt
    32221 gttttgaacg atgttaacga agatgtattg tatcagattc ttaaaaatcg ttacagtacg
    32281 gtagttaatg gcaaaaaact cgattttctg gcttacggta ttaggctctt cttcgaagat
    32341 gaggctttgc gtatattggc aaagagggca gcttcggaac atactggggc aagaggtttg
    32401 cttagcgtat ttgaaaaagt tttgattaag tatgaaaaaa taatgccgtc acttcattat
    32461 gaagagcttg tcgttgatgc tgatctggtg aataatccgg aaaagaagct aaaagagcta
    32521 ttgttaaatg acaatataaa gggatttcag tctgattttt tggcagctca tggaatatat
    32581 ctggattttt cggatgaggc aatggagctt atacaacaaa gggcttcggt tgaacacaaa
    32641 agttttaaag caatatgtga ggatcttttt cgcgattatg cttatgccat taaattatta
    32701 aaacgggagc agtttacaat tactgcggag gcggtacaag attccgcagg ttatattgaa
    32761 aaatatataa aagataacta taaaaagagt tcagggaatg catcacagga tgatactgat
    32821 tttgtttaag ataacgcatt aagctgtttg gaatatcagt aaatatgggc aaataaagtt
    32881 aaactattcg atatatttac ctagttcctc ggcgtaagcg gtactgtttt ttataaagtc
    32941 agtttcccaa gccgaaataa atactgaaaa ggattctttg taatatggga gtggatagca
    33001 tgctgttatt acaaggtcgt tttgcgagtc aattgtgtat tttgagacta tcgaactgat
    33061 atttattcta tttacaagtt caagataagc cgtcttttgt gataaagaat tttttttgtg
    33121 tttaaaccaa gcacgaagca ctattcctcc acgtgtttga attatatcaa aaccgggttt
    33181 ggtcgggtgc tttactatta tagtaccatt gttgatttta gcaagatagc caagatatga
    33241 cagatggtcg atgatttttt tatggactac cgattgttca gcagcattga tatttaagca
    33301 gactatgaat attgcaatag tcagtttttt catgttttct cccaacaaat taaggctatg
    33361 gattatgtta atggagtgat tatttttgta aacgtaaaat aaataaaaat ttcagataat
    33421 gcttaattgt atgtctgtta aaactgtatt tgaggttttt atgttcggtt cttataaaaa
    33481 gatagtattt cttgtttttc ttgttactat ttcaagtttg tcctcacagg aaaaatctgt
    33541 tgatcgacct agattgatag ataaaacatc tttaaaagct ccagcttctt ttactaaaca
    33601 atcagtaaaa tcagcggttt ttgatattca catggcaaga gtgactcttt gtagccgctc
    33661 ttttgaacag ggagatttgg catatcttga aatagtacct caggatgagt ctaatcttga
    33721 tagtataaag ataacatatt atggtaaaaa aaagagtaaa cgaactattg catatattcc
    33781 ggtaacacga ttgaaatggg ggtatcgtgc ttttattccg ttggatcccg actcggatcc
    33841 tggtaaacac acggtttctc tttactataa agcaaatggg cgtgaagtcc agaaaaatct
    33901 tgtgtttaag gttttaaaac gtaattttga gaaaagtact tacccgttat attttaaagg
    33961 ttattcaact gctgggaaat taaaacccga gactaaaaaa tttatcgaag aatccagaat
    34021 aaaaaaagat aaggctttcg cgtcaagaga agatgactat ataaataaca gtctgtcgca
    34081 tccgagagac atgcattata taacatcatc tttttgggca aaacgaacat ataaaagata
    34141 cagaagactt aaaaacggta agcgaaagcg gttaaaggat actgtaaaaa ttcatagggg
    34201 cattgactta agaggaaaaa gggggactcc tgtttacgca atggctcgag gcaagatagt
    34261 gctcgcggat aagctctatt atgagggtaa tatggtaatt attgaccatg gtaaccggat
    34321 attttcttat tacatgcatc ttgacagttt cagtgtaaac gagggtgata ttgtttcctc
    34381 gggttgtgag ttagggacgg tgggatcaac tggaagatcg actgctccgc atcttcatgt
    34441 ctctttggtg gttgccggtg tgcaggtaga tcctctttcg cttctagttt tgcctattag
    34501 atgattcatt atgtgttgtt gttttaaaaa ttaaaatctt tatggcaaaa atattttttt
    34561 aaaagaagga ttttaagata ttacagtata aaatagactt tatagaattt ataatgtttg
    34621 atataatgac tctgttttgc attttaggag gaaaacaaag gatgaaattt gataacttta
    34681 ctcgtgtaga aggcgattct tgcggaacaa ttcagctgtt tggactatca acctgtattt
    34741 ggtgcaagaa gactaaagaa tatctgaata gtaagggtat tgagtactac tatgtttttg
    34801 ttgatttatt gaatgagagt gaaaaagaag aagctaaaaa actgattaaa aaatggaata
    34861 atgatatttc atatccaacg ataataataa atgaaagtcg ttcagttatc ggatttgatg
    34921 aagatgagat agaccgggag attgcacgca atgagtaaaa acaattcgaa tttagaagat
    34981 tctgaaattg acggtcttta ccgaatagtt gagcgtgatg ctcatgaggg cgggtatttg
    35041 ctgaatcctg atgattcatt tacacgagaa ttgattcggg gattggttgt aaacaccaag
    35101 cgctatgggt atgcggcatg tccttgcaga ttagcatctg gtgatgaggc cgaagaccgg
    35161 gatatggtat gcccttgtga ttatcgtgat gcagatttga atgagtacgg agcctgttat
    35221 tgtgctctgt atgttactga agaagttgct tctggtaagc gaaagttaag gccattaccg
    35281 gatagacggg aaagagagtt ggaaaagaaa aaaaaagata aaggtgaatc agaaaaaaat
    35341 atttcaggat tatcttatcc ggtatggaga tgtagtgtgt gcggttatct tgcggctaga
    35401 agtatgccgc ctgaaaagtg ccctatttgt aaagtgtcaa aagagcgatt tgaaaaattt
    35461 atctgattga attgattatg cgtaaatgag gatgatttat ttgttttttt gatctaatat
    35521 tataaataaa atttatataa tggcaggtgt gatatgatta agcatatagt catgtggaaa
    35581 cttaaagacg atgttgatgg tttaacaaag gatcaaacag ctcttttgtt aaaaaaagaa
    35641 attgaattaa tgaaagaaaa aattccgcaa atagttaaaa ttgaggctgg aattaattat
    35701 aatcacggac ctattgcgta tgatctttgt ctttattcag aatttgaaaa cgatgacgat
    35761 cttgccattt accaggatca tccggatcat gcggttgtta aagaacttgt tgtaaaatat
    35821 gctgaaaacg gagttgtggt tgactatgag atataaaata atattcaata tattattgtt
    35881 gtgtattgca ctttctgttt tatcttgtat taaaaacaat gaagagagta gtaaaaataa
    35941 taaggagaaa aagatactaa tgaatggcga aatagtaata acacttgatt cagaaaaagc
    36001 acctttgtcg gccgaaaact ttatatcata tgttaaggat ggtttttttg acggtacaat
    36061 atttcatcgt gtaataccgg gattcgttgt acaggggggg ggatttactc aggaaatgga
    36121 tcagaaaaaa acaaaagatc ctgtaaaaaa cgaagcagat aacggattat caaatgttcg
    36181 tggatcagta gcgatggctc gaacacaaat tgttgattcg gctacttctc agttttatat
    36241 aaatttaaaa gacaatgtaa tgcttgatca taaaggaaaa gacgaacaga attacggcta
    36301 ttgtgtattt ggtcatatta catcgggaat ggaatttgta gataaaattg ctggagtcaa
    36361 aactggaaac tctggttttc atcgagatgt tcctgagata ccagtagtta tcaagagtgc
    36421 agtattgggt gacaatggta agactgttac gtttacaata gaacaagcca cagagtaggt
    36481 attatggaaa accgtgacat cgagcagtat ttcaaagaga tgatgagtgc cgaaaaatcg
    36541 gctgaccatt cgattattac ttctcttgag aaaaaagttt atgatatgaa aaatttaata
    36601 gagattggga ttagtctatc atcaaatctt gattttaaga gtcttgttga cgcggttttg
    36661 tatagttgta tagggcaact gtttatagat aaaattgcta ttcttttaca agttgatatc
    36721 gattcgaact gttattatat acattcacat aaaggatatg atattacttc cataagtgat
    36781 gaaattatcc tgggagaaaa ttctgttttg agtagttttc ttcaggatca ccctgagcca
    36841 catgattatg aggctttaaa aaaaattccc gagctttctg aagatctaag tgcagtagat
    36901 gatttatccc caatattaat tgtaccgatg aagtcaaaga atagtcttaa tggtataata
    36961 atagtcggga acaaaatgct cggtacaccg tttactaccg atgaaaagga gtttttacgg
    37021 cagttgtcaa ggttttcagc gactgctgtt gaaaattctc gactctatct gatggccact
    37081 caggatcgaa tgactcgttt acatgtacat catttttttc aagacaagtt attggaagag
    37141 atgaagcgct ctcagcgaag cggcgagcct ctctctctgt ttataacgga tattgaccat
    37201 tttaaaaagt ttaatgatgt gtatggtcat cagcaggggg atactgtatt gaaagaggta
    37261 gcacgtttga tgcgtgaatc ggtgagagca actgactgtg ctgcacgtta tgggggggaa
    37321 gagtttgctg tgattttgcc cggcgcttcg cttgagttgg cttctgatgt tgccaatcga
    37381 ttacgtaaga caattgaaca gcatgagttt ccggggcaaa atgagccttt gcatgttgcg
    37441 atatcagccg gcgtcgcaca gtacgatccg gaatgtgatc tacataagga agatcttata
    37501 gaacgtgctg ataaggctct ttacaaggcc aaggagtctg gaagaaacag ggttgctgtt
    37561 tttagatgaa attattttaa cgttatatta aaaaaagtga tgaacaaaaa aagttcatca
    37621 cttttgctat tgcaaactat taaattgtca aatgtaatat gactaattca gttcaaaata
    37681 taaatcttac tttcgatcaa gagttgtagt cccgtcaata atccactctg taaaatccca
    37741 gtattcacca gatttaacaa gggtgaaagt gtttttatgc tccattccta aatcatcatc
    37801 atggtatgca gttgcggaac cagtgtctcc actttcggac gttgagatgt ctgtaaattc
    37861 aagattagct gttcccacgg aatcttttaa agtttccata cttgctatgc ccgaaccccc
    37921 ggcattggag ccttctgaaa agagtactgt aatttcagcc tcacttcctg aattgtaggc
    37981 atcctgaagg tcgagaaccg cattctttat gccatcagtt tcgctctcat cgtcttcaca
    38041 ggaaataaag atgagtaatg caaggattat agatgtaatt gtaaataatt ttttcattta
    38101 tttggtctcc taataataac attttaacgt ctacttcgtt tttttctaag ctcatctctc
    38161 ttttgttttg cttcaactgc tgctttgatg tatttaatat agtacttttt ataaagatcg
    38221 tctatttcca tatataattt attaatttct gctgtacgat tagatcgatc aatggtttcg
    38281 tatgttaaat ccgaaaaatc tttaaccatg cccattttat aggcgtttac tatcgcccta
    38341 aaagaggcga gatgaagttt ctgagctgcc tgaaaggaga gttttgcatt ttcctcaaat
    38401 ttggctgtgt ttccatcggc tttgaatttt tttgccaact catcgtattt gatcttttct
    38461 ttctggttaa actcttttat ttcttccagt tctgttatat cattgtcaag ttttttcatt
    38521 tttttcaaag attcgttaag aactgattct tcattgcctt taagctgatt ttttgagtca
    38581 ctaatccatt tgttccactc ggagatttta atgtcttcaa cttctgatgg gtcacctcca
    38641 tatggcacag tggtttcctg gttagcgttt acaagcacag ttcgtgaaga acctgtaata
    38701 gctacccgtc ctttttgtac ctgtagaaca gatgttccgt cataacctgc tgcaacaaga
    38761 aattctgtac ctctaacagc acagactgtc gacacagtat taactttaaa acctgaatta
    38821 ttgttttgaa gcttgtccat tttaagttga actgtgccac gcgtcatatc gagagattgc
    38881 actttcttct cttcatcgat atcctgtcca atcgctaatt gagtattttc attaacctta
    38941 atagtgttgc cgtccatatc gatctgaatg taggcctttg agcctgtttt aacggtatca
    39001 ccgttaacaa gtttttggtt catactggct ttttgccaag tatcagagcc gaatggcttg
    39061 attttcgcct ctccgacaaa aaactttatc tgcatggcag aaaatgccgg taaggaaaag
    39121 gtcatggcga atagtattaa taggagtttt tttacagtaa tcatatctta ccttcttttt
    39181 tgttggatta accgttttgc gatgaataaa ataatattaa aatacggaaa atcccttttt
    39241 ttaagtaaaa gaaagaccat cttttggaac aaaagtaatt gtatagttac aaaaaatgat
    39301 taaaacaaaa tctttagcat ccatttattc attgttatac ggtattggct aattttcaag
    39361 ccaattaatc atttttctaa atttaaggca ctttattgct cttgacattg aacataattt
    39421 gaagatatgt cgtattaata tgactgttat cggttgcatt tgcaatttta taattttgcc
    39481 tgaatagata atatgattct cttgacgtaa ttttatgatt atctgtatat aagtcatacg
    39541 ggggtaattt atggaaaaag tagagataaa aagtgctgat tttacagctt tttttaccgc
    39601 tgaagatatt atctgtcata gtgatttaaa aaaacgaaat gatttgtttt ctgcaatatt
    39661 gaaaaatctt gcttataata aaggcattgg aaatgtaaat gatgcgtata atgcagtgct
    39721 cgagcgggaa gagggttata gtacagtcct ctctaaagga cttgctctac ctcatgctag
    39781 gttatcaaca attagtgagt tggtaatagg aattgctaca tcgaaagagg ggattgtttt
    39841 tgatggtgat tctgagaatc ctgttcatgt tgttattatg atactcacgc ctaaagataa
    39901 gccttcgctc tatttacagg ttttaagttc tgtatcaaag gtacttgccc aagaggggat
    39961 aattgataaa cttgctgagt tttctgataa taatgagatg tggcgttttt taaaccgaag
    40021 cgggcttgat tttccggatt atatttgtgc tggagatatc atgaatagtg atttttcgtc
    40081 tttaaaagaa aacgctacgc ttgaacaggc aatagatctt tttgttaaca aaaatatgaa
    40141 cgatgttccc gttctagatg cagatggtga tcttgtaggt attgtttcga caagtgaatt
    40201 attgagagta tgtcttcccg attatattct atggatggat gatctatctc ctattataaa
    40261 ttttgaacct ttttcgcata ttcttcaaaa tgagagtaat acttggcttg cggaaattat
    40321 gtcgtatgat tttgcatcgg tagatgaaaa agatccagcg attatggttg ctcaggaaat
    40381 aacaaaacat aaaaaaggtc aggcctatgt tttgcgtgat aaaaaattgg taggtgtgat
    40441 taccatgcag caatttttaa acaagatact cagggagtaa aaaatgcata gtgataatga
    40501 aaaaataaca ccccgccaat ccatgatcag gggaggcata cttcttgccg taactttttt
    40561 tgctggatgg ctggcatact acgcgggatt aacagaaaat tcacaagtta tggctgcgtc
    40621 agtatttgtt ttgataatag gtgctactct gcttttctgg aacgcacgcc ttgcaatagc
    40681 ttttattggt attgccatat tattaggtgg gcgggttttg actctggaac agatgattgt
    40741 atcggcagaa cttgatatca ttttattttt ggttggcatg atgactatgg ttggtgtgtt
    40801 gaaagacctt ggtcttttta cttggattat acagcttgtt ataaatatga aagctatgaa
    40861 tggcaaacta ttttgtataa ttgtggttgt gatgtcggct gtaatggcga gtgttgtaga
    40921 cgaggttact tcgattgttt ttattttagc acttattttt caggtttgcg atacactgaa
    40981 aattaatccc gtacccttcg taatgattgc ggttatagga acaaatattg gatcaagcgg
    41041 aactatgctt gggaatcctg ttggcatttt aattggtaca aaggccggat tctcttttgc
    41101 tgattttgtt cgctgggcag ctccggtaat gtttgtatcc cttttggctg cgctttttat
    41161 tgtactagtc tggtatcgaa aagatataca acttcttaca gagagattgg aggcacgtag
    41221 aaagatgcaa cttggtcttg ggccgcttgt ccggattccc tttaaacgtg ggcttttcat
    41281 tcttgtgtct gctgttactt tgattgcttt gcatcataag ttggaaactc ttcttggggt
    41341 acagaaaaat acacttttaa ttgtagcgcc tctagcaata tctggaattt tgatggtttg
    41401 gagaagtaat cgtgcaaggc attatataga agcagaagtt gagtggtgga ctttgctttt
    41461 ttttatgatg ctttttgctg tagcgggttc gcttgaacat acaggtgtaa cagaacatgt
    41521 tgcgcataac tttgttggaa cgtttggaaa taatcctgtt gtactaactc cgattataat
    41581 atttgtgaca gcgctcggtt cggcatttgt tgacaatatc gtttttgttg cggcattcat
    41641 acctgttatt aaggaattaa atatgacacc cttatggtgg gccatgcttt ttggtgcctg
    41701 tttcggtgga aatattactg ctattggctc taccgcgaat atagttgcgt tgggtatgat
    41761 ggaaaaaaga tataaaaagc atatagcttt ttttgaatgg ataaaaatag gtgctgttgt
    41821 tggatttgtt acctgtgtgg ttgcctggat attgattctt ctgttgatgc catatatgcc
    41881 ggaaatgaat caaataggac attaatttag acatgcctgg ggtgttattt gcgcttacaa
    41941 gttatttgct gaggtgatag caggtttgaa atatttgtta gtccgaggtt attatactga
    42001 cttgtttaga aagttctttt ttatctttat tgcagtatcg ctattttgtt taaactgttg
    42061 tacaggtaat gttgaacgtg attctgaggt gctatcaagg aatattgttg aaactgtaaa
    42121 tggtatcatt tatgagttca agggaaataa aaacactctt gttttttacc ttgaaggtag
    42181 tggattgcat tcggtacttg gggtaaaaaa gaatgatggc tggagccata gaactttttc
    42241 tttttttgtt ataaaatatt ttaatgcctt tgctgatgtt gttgttatag aaaaaccgaa
    42301 aatgaaacca ggtaaagatc attccactga cggtgaagta ctggttgcgt atacttcaag
    42361 taatttaatt gatcactatt ctgtcatgat tgacgattat cttgataagt cagacgctaa
    42421 gcaagttgtt atttttggat attctgaagg tggaatgctt gctccagcag tttatagaaa
    42481 tctgaaaaac agtgatcgta ttaataaact tgtaatcggt ggtgcaggcg gcatgagtca
    42541 gtacgagcaa tttatggtgc ttgccgagtc ttatgtaaag atgccggata aataccgtct
    42601 tgagtgccgg gatattcaaa gagttagaag ccgggttagg acttttcctg attcaattga
    42661 atttagtttt ttgggattac cgtacagaag atggaacagt ttttttgatt tttctcctgc
    42721 tgattattat gcaaatatcg aaataccggt attattttat catggcactt ttgactggag
    42781 tgtgccggtt gaatcgacta gtatgcttga aaaattatat cctgataaga attttaattt
    42841 tatgtatatc gataagatgc agcatgtgcc agaggatgat aaggg
//
LOCUS       DAOTGZ010000003        42493 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_3064
            NODE_945_length_42493_cov_6.931830, whole genome shotgun sequence.
ACCESSION   DAOTGZ010000003 DAOTGZ010000000
VERSION     DAOTGZ010000003.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868088
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 42493)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 42493)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:22:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,785
            CDSs (total)                      :: 2,751
            Genes (coding)                    :: 2,732
            CDSs (with protein)               :: 2,732
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 12 of 19
            Pseudo Genes (internal stop)      :: 5 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..42493
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_945_length_42493_cov_6.931830"
                     /isolate="AMR_MDS_3064"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..460
                     /locus_tag="P5123_00395"
     CDS             <1..460
                     /locus_tag="P5123_00395"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRX14751.1"
                     /translation="SIVFMCTILIAQDNQNSNQHDEANPYWPMNVIFTNATTASFADP
                     YNEEKSKVAKAIEDTNNKLMEKIDSKRDEFETLFLAKKKDRKKIIQCTKELYALQIDL
                     ELANMEARKKMDDIDRKMVDELTGKVNIWTEEMLKDESKYKRFINELKPD"
     gene            483..1358
                     /gene="argB"
                     /locus_tag="P5123_00400"
     CDS             483..1358
                     /gene="argB"
                     /locus_tag="P5123_00400"
                     /EC_number="2.7.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013537251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003991 - acetylglutamate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006592 - ornithine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetylglutamate kinase"
                     /protein_id="HRX14752.1"
                     /translation="MQKYIDKINTLIESFPYIKKFSGKTFVIKYGGNAMVEDKLKRAF
                     ALDMVLLKYVGIRPVIVHGGGPQIANLLSRVGKESVFQNGLRVTDSETMDIAEMVLVG
                     QVNKEIVNMINMEGGRAVGLSGKDANLILADKIYHQDGENQVDIGHVGTVRKVNPSII
                     ETLDKENYIPVIAPIGVNEQGQTFNINADTAAGKIAEALNAEKLILLTDIEGIKKDDK
                     LVSSATIDEIKQMISDKVIKGGMIPKTECCISAITNGVHKTSIIDGRVEHSVLLEIFT
                     DSGIGSEIMLKHEDI"
     gene            1345..2733
                     /locus_tag="P5123_00405"
     CDS             1345..2733
                     /locus_tag="P5123_00405"
                     /inference="COORDINATES: protein motif:HMM:NF027464.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4846 domain-containing protein"
                     /protein_id="HRX14753.1"
                     /translation="MKIYNSFKYKRGLTIFATAFFFIPFLTVLISNSCTKETSKSFET
                     EDNPFTLIMTNDTYIASELSMPVGKPPLKDYYNAQNFGIRNKNFDNKYHLGEDWNGIG
                     GGDTDLGDPVYSIGNGLVIFAANAGPGWGNVVMILHRLKNDKLICSLYGHLHDYHVKT
                     GDFVKHRFQIGNIGNADGLYYAHLHFELREDISLGVEGGYSNSTKGYLNPTDFIISYN
                     NQFLESPFLIKDGSSVIERFLTPAGFQRELFRKGSYENFIQNLPLKPYGHKVRYFNKK
                     IKEPKGIYISVLDYDTGDRDLLQCADAVMLLRAEYFFNKSDFSNIHFTFLSDGKPRFY
                     NSFGGKLNDYNNFRKYMNYIFSYANTASLIKELRPVKIMDVKPGDVFIQKGSPYGHAI
                     IVIDTAVSASGEKMFMLAQSFMPAQEIQILINPTDSTISPWYRTDSKKIITPEWVFRS
                     DDLKRFNKPNNL"
     gene            2847..4445
                     /locus_tag="P5123_00410"
     CDS             2847..4445
                     /locus_tag="P5123_00410"
                     /inference="COORDINATES: protein motif:HMM:NF014801.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016773 - phosphotransferase activity,
                     alcohol group as acceptor [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FGGY family carbohydrate kinase"
                     /protein_id="HRX14754.1"
                     /translation="MKRLSLGLDSSTQSMTGVLIDIDNGEIVYSKSLDYASDPRLNCY
                     GINHKEYLVPPREAGEADQPPLMYIASIDALFEDMKKDGIDMSAIKVINTSGQQHGHL
                     YLNKKAKSYFSSLNNAGNDSGDLVSIIGSSFSYQTAPIWKTSNTETQANAIREAAGGK
                     DKMIMISGSDSPLRFTGAVIRRVGEQFPQVYQQTEKIQLISSFIPAVLCGNSEVPIDF
                     GNACGMSLMNYEQRNWSNTLIDAVSSSLPGGNSSLTAKLPDITSPDSIVGSIATYFKE
                     KYGFNECVIAAGSGDNPQTKVLIKGDLLSLGTSFVNMVATDGTSYDMGGFANAMYDGV
                     GRPFMFGCRTNGAMVWDRVRANYGLTKKDYKKAEEALKSAPPANGIFMWQVDNESFPP
                     SKKFDPVRIGSDSGSLEFDYSGIIDSSLAAVFTYSQAFARKTSEPLYITGGPTSSAGI
                     MRRAAGIWGRPVVTIGSVGAGLGTAVAGAQALSKHTKESFDAEQLSASVLPRGEAINP
                     IEEDVKKYKEYLPKFKETFEKYAM"
     gene            complement(4499..6265)
                     /locus_tag="P5123_00415"
     CDS             complement(4499..6265)
                     /locus_tag="P5123_00415"
                     /inference="COORDINATES: protein motif:HMM:NF012437.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate/guanylate cyclase domain-containing
                     protein"
                     /protein_id="HRX14755.1"
                     /translation="MKKKYNKEHDKNKLSSCRIFYVFIIGFFLLSCVLFGLIGFKWIM
                     SNVQLSGNNQIVEPLLKSMEHIYVLVSFVLFMAVSLIMLPFAKKKGLDIKRQRSKRLF
                     YSPLLSAGFLLVVWAVPLFYLVYFVVLNKSFDFVIAGMFLSLILICLGVSSISFVICD
                     YLVARIFIPRFVDNSDVNSCSSAFLPGLIFKSIMLFISTIVVPSAILMQYIYNISIGS
                     KNIEAFDGIKTYYIIFLVVTSVLYALIVKYHRGLILSMTNVITKMVRGDYSGRNLITS
                     SDEVGFLAEIINNSVISLKEKEFIKDTFGDMVDPVVRNFLLKGSLNLEGDKVFVTLMV
                     VDICNYTDTVSDLKAEDQITYLNIFFDRMSGLIESTGGLVNKFIADSLLAVFGAPVSN
                     GKHSNIALECADNLRDYLITLNRELSEKNLPLIDYSIGLHTGEVIAGNVGSKSRMQYT
                     ITGKSVNTAFGISQIAQMYRVPVLLSKSTYSDLDNSDDLNLRYVDCVKLKGETEPVTV
                     YEYFESDKEKNKKHKKRYLSEYNKGIEYYRDGKFNEALKCFLYCHEEMPWDYLSAMYI
                     KRCNKLIKKPPTNWSSVAIINR"
     gene            complement(6297..6983)
                     /locus_tag="P5123_00420"
     CDS             complement(6297..6983)
                     /locus_tag="P5123_00420"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012745.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HRX14756.1"
                     /translation="MELIALPIYNQATITPRRIAELYTYVQGVNDVLIVDDGSDDGIG
                     DLIKTGEKLKYIRHESPLGYGGSFITVMEYAFKHDFERVYFLDISNENFTEPVVLMRE
                     ILDSGADIVNISRKQMKQKTDDYLIINPSEPVSQKINSVTGFCLNDVFSPFKGFAINS
                     LKEMTLEEFDESLILQLWIQSAHYKKKVREVYCSKILDGTINDISILSHDPEYYLNFI
                     QGELLLYPQN"
     gene            complement(6983..7483)
                     /locus_tag="P5123_00425"
     CDS             complement(6983..7483)
                     /locus_tag="P5123_00425"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2127 domain-containing protein"
                     /protein_id="HRX14757.1"
                     /translation="MKKPGITLVSVIYFIFTLLCSLICLSYGIALVLEHFGFASNLWF
                     TIINMVFKFFFSDLIIMIFIGFAIGVVSAWVSLQLLKRKKWARLFFICFSIILIPWIV
                     YRLINAFTDMPFYVSLEAIFGSNPLTKYPSIIYFFIPFVLVLLEILLVYTIVLLVGKT
                     ARQEFK"
     gene            7668..8678
                     /locus_tag="P5123_00430"
     CDS             7668..8678
                     /locus_tag="P5123_00430"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRX14758.1"
                     /translation="MSEEITEIYKHNIFIKCRFKSSARNLYGLILIDFNPSGAVDNYQ
                     AIASTFSEIYEINPQSGWEDYEDQIVQTKWNGKYNNNITRDFQLQLDSLFDDTFQRTQ
                     LFEAANDYDYGSAEQILYDTARRILQDKNLKLELNIQEVSADEITMIRNQRETERQQK
                     YQQDKESEFSIEEGSVVLNSSLVLSPVNGKPLYDLKIGERIMIKLDPSSSKNQQYISL
                     YNLKTDANTYLSVPAEVIDIKAASKAEPVQILTRIEDSVYAKAVEDERQVKLRMFNAA
                     TDRSIQVMKNSSKKAVSTQTIANYNRQDSVLNENSSITIVLALALALLLFLIIIAIYL
                     LL"
     gene            8703..10031
                     /locus_tag="P5123_00435"
     CDS             8703..10031
                     /locus_tag="P5123_00435"
                     /EC_number="6.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011309506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004356 - glutamate-ammonia ligase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006542 - glutamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine synthetase family protein"
                     /protein_id="HRX14759.1"
                     /translation="MDRDKDYIIKLSHEKNVKFIKLWFTDILGMLKSFTITIDELEDA
                     LCEGIRFDGATLGGFIRTNEHEMIALPDASTFQILPWRPNEDSVARMFCDIYTSDMKH
                     YEEDSRYILRKTLKAASDAGFTFYTGPEIEFFFFKSTEKPELLDKGGYFDLTPLDLAA
                     DYRRMTVLTLEKMGINVISSHHEGAHSQHEIDLRHEDALTTADNIMTFRIVAKEIAQV
                     NNIYASFMPKPLSYQNGSGMHLHLSLFDKDDNNAFFSTKDKNNLSDTAKHFIAGLLTY
                     VPEFTAITNQWSNSYKRFVYGHEAPTHISWSNCLNSALIRIPEMRKEKPDSMRIELRN
                     PDPSCNPYLVFSAILAAGMDGINNKLELEPALEDLQNNSKIADPAKLGCKKLPSHLSE
                     ALLNLQKSDFMKKTLGTKVIEKFIENKVLEIAEQNSYVTDYDIMKYYPLL"
     gene            10109..10429
                     /locus_tag="P5123_00440"
     CDS             10109..10429
                     /locus_tag="P5123_00440"
                     /inference="COORDINATES: protein motif:HMM:TIGR00377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043856 - anti-sigma factor antagonist
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="STAS domain-containing protein"
                     /protein_id="HRX14760.1"
                     /translation="MKITVNTQNNTYFLILDGKFNIDHIDVFDENFHSIIKNKPDAIA
                     INLQNVEYIDSSALGSLIKGLNLCKKLNIKFYLCDLKENINLVFKLSYLDKFFNIVSS
                     SEIN"
     gene            complement(10457..11098)
                     /locus_tag="P5123_00445"
     CDS             complement(10457..11098)
                     /locus_tag="P5123_00445"
                     /EC_number="2.5.1.9"
                     /inference="COORDINATES: protein motif:HMM:TIGR00187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="riboflavin synthase"
                     /protein_id="HRX14761.1"
                     /translation="MFTGIVEEIGKVSEIRKESNGVRLGIRSETVISDANIGDSIAIN
                     GVCQTVESINGDIFTVFVSRVTLDLTNLSNLKTEDNVNLERALTTSSRMGGHIVQGHI
                     DGTGNVLTVNKSINAIDFRIGCSSKLMMYIVEKGSVCVNGVSLTVVSVFEGGFSIYLI
                     PESIKKTNLYSAVAGNKVNIEVDILAKYIEKMVTNDSTKEDFLDRLERNGFLG"
     gene            complement(11098..12249)
                     /gene="ribD"
                     /locus_tag="P5123_00450"
     CDS             complement(11098..12249)
                     /gene="ribD"
                     /locus_tag="P5123_00450"
                     /EC_number="1.1.1.193"
                     /EC_number="3.5.4.26"
                     /inference="COORDINATES: protein motif:HMM:TIGR00326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008703 -
                     5-amino-6-(5-phosphoribosylamino)uracil reductase activity
                     [Evidence IEA];
                     GO_function: GO:0008835 -
                     diaminohydroxyphosphoribosylaminopyrimidine deaminase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     diaminohydroxyphosphoribosylaminopyrimidine
                     deaminase/5-amino-6-(5-phosphoribosylamino)uracil
                     reductase RibD"
                     /protein_id="HRX14762.1"
                     /translation="MNPDTLFMEKALKMAFSSVGITSPNPPVGAVIVKNNKIIGSGST
                     STFGGNHAEVNAINSCTESTEDSVMYVTLEPCCHFGKTPPCTMAIIAAKIKKVVIAAI
                     DPNPLVCGKGILELENAGIETIIDKKNGSFARELLEPFKTYLEEKRPYIVHKAAMTLD
                     GCTASHNKDSKWITSESSRALSHRLRSVVDAVIVGKGTYIVDDPRLDVRNEYLDNKKV
                     NVYSNESFLFSELISNDFEVKKRTPLRVLAGFPDRFENRSFFYDDNYLIFCSESDHDC
                     DIPEYVDREKIIPIGFSGKKFIEKMLKILYDQGVMFAMVEGGARLNASFHEAVLPDSY
                     LYFFAPKVLGGGHPLFKGSGEEMIKNSRTVNHKALALIDGDLAVYGEFK"
     gene            complement(12262..13161)
                     /gene="secF"
                     /locus_tag="P5123_00455"
     CDS             complement(12262..13161)
                     /gene="secF"
                     /locus_tag="P5123_00455"
                     /inference="COORDINATES: protein motif:HMM:TIGR00966.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031522 - cell envelope Sec protein
                     transport complex [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein translocase subunit SecF"
                     /protein_id="HRX14763.1"
                     /translation="MKQKILPFLRFKYVAYAVSAGLMALFLAITIPQGGLNWGIDFVG
                     GVKIRAQFESGVSISDVRSTLQKSGLNASVQTIGNDENNEFVISTKLIEGSENTESTY
                     ELLWNTLKADYPAVVQLANDSVGPTIGDYLKQSAFKLVIIAVVLMMIYLAFRFEFKYA
                     LGAMIALAHDLVLAVLFCGMMNVEINVQVIAALLTIFGYSVNDTIVIFDRIRENAENE
                     TSPIFADIINIGISQSISRTLLTSLTTLFAVLSLYLLGGDTINDFAMVLLFGIFVGTY
                     SSIYVASPVVLKYTKIMDKIKHK"
     gene            complement(13217..14869)
                     /gene="secD"
                     /locus_tag="P5123_00460"
     CDS             complement(13217..14869)
                     /gene="secD"
                     /locus_tag="P5123_00460"
                     /inference="COORDINATES: protein motif:HMM:TIGR01129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031522 - cell envelope Sec protein
                     transport complex [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein translocase subunit SecD"
                     /protein_id="HRX14764.1"
                     /translation="MVKGFKGRFIIILLVTVLSVLIILPTFAEKTLKLTVVSGEMDRA
                     KKELASRFSEDSYTFDYEGNNTIYMKGYSLTHAVMNEVKMLFDYVEEAEFETHWAEKY
                     LLASKIKLGLDLQGGMNLVIQADYKKMEEKIGRPLKEEDKKRITAQAIEKINSRINMF
                     GVSESVVRPKGTEAIELQLPGVSDPQTVKNTIGNTGQVEYRIVDDEWTEKVNNYHKAN
                     NIKLPNDRFKLRDIATEIAVATGLPENFQIFYQYERNPDDGKLYPSYPMVLSKTVSMS
                     GDDIKRANVTQDQFQKPAVSFELTTKGAEKFAKVTGENVNKRMAIVIDEQVRSAPNIN
                     ERIVSSSAQITGNFTYEESEILASIIEEGALPVELIFVEERTVGPSLGQDSITAGFWA
                     IVIGLAGIMVFMLIYYKFSGLLANLCLLLNMLFLLAIFSWIKATLTLPGIAGLILTVG
                     MSVDANVIIYERIKEEVKNGKSPRVAVSNGFGRAFWTIFDANLTTLIAAFILSQVGTG
                     PIKGFAVTLAFGIVCSMFASLYVSRFIFELITAKTSIKKLSI"
     gene            complement(14883..15452)
                     /locus_tag="P5123_00465"
     CDS             complement(14883..15452)
                     /locus_tag="P5123_00465"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRX14765.1"
                     /translation="MICRRVYIFALIALLSFSSLICQTTDDVATEEEISSEELVDSSD
                     ESVVTTPKTDKEEASRKKEPVPAPKTVAPSSGLVLLDITDALLYDSRVPDIELEKREV
                     MIADIDEPSVEEVPDKGDNVATKNEVVATEDGTKKDGSFRTMNRAVKLWLPRVLIILL
                     VLILIVLYNSKSKKKRRRVFRKIPSKRRL"
     gene            complement(15457..15801)
                     /gene="yajC"
                     /locus_tag="P5123_00470"
     CDS             complement(15457..15801)
                     /gene="yajC"
                     /locus_tag="P5123_00470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009708268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit YajC"
                     /protein_id="HRX14766.1"
                     /translation="MNFTVMAQAGKAAPASGGLISFAPFVLIIVIFYFLIIRPQQKKE
                     KERKRMINELRVGDKVLTIGGIQGVITGIRENEDIVTVKISDNAKVDFIRSAIQNKVL
                     PPSADDRNKAKK"
     gene            complement(15935..16339)
                     /locus_tag="P5123_00475"
     CDS             complement(15935..16339)
                     /locus_tag="P5123_00475"
                     /inference="COORDINATES: protein motif:HMM:NF015871.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OmpH family outer membrane protein"
                     /protein_id="HRX14767.1"
                     /translation="MKPLVLFFVKFFCLIFFVSCNALVKTESPSFSTAYVDLEVLYHF
                     VLQNDPDYKMYQQNSEKPVSIKLLNNSDEIKRSVALKVEDAVRYIASENGFKLVINKK
                     STIVYADKQLDITQSVINELRLRHNRRSLDKR"
     gene            complement(16427..19093)
                     /gene="bamA"
                     /locus_tag="P5123_00480"
     CDS             complement(16427..19093)
                     /gene="bamA"
                     /locus_tag="P5123_00480"
                     /inference="COORDINATES: protein motif:HMM:TIGR03303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamA"
                     /protein_id="HRX14768.1"
                     /translation="MIKKPLISFYIILIIAAIPFFKGGLLMAQEQMPSRFKGKTIVNV
                     DFIGKKKLENGKEEDIAIRNLDKADLEAHVLTEKGYPLDPKVITDDINALFKKADLLD
                     VRAEVTEQGEGVVVRFIIIERPVISMINFLGMDTLSETDLSEEIPVKIGESYREFQIP
                     VAEKIIREKYIEEGLFNVMVTTVIKHVDDEENNNVTVSFRIDEGEDIAVHKITLFGAY
                     KLGDDLIRNVIETKEKSLFTESKFNVATYEADKQKILTLYRQEGYLDADIIDEEVVYD
                     WVDPEKKEKRGIFITLRINEGERYYFEKYTVEGNSILSTERIMKLFELKHQEETPLTR
                     FADKVLKLVGQDVEKDTICNDTFFQMDRYNLAFEYGKQGYLFTRIMPEKNIIEREVVI
                     DGRKEIRKYVHYHLTIAEGTQSYIENIIVSGNDKTKEHVIRREILFKEGELYDAEKIQ
                     VTREVLYRLGYFEEVTIDIRPGSSNEKVNIIVGVKEQNTGTLSLGGGFSSDVGFSIFA
                     SVAENNLFGYGYRAEVKVEYGEYNKSATVTFTDPWFYQIGDNPVSLTLSIFYSLQKIR
                     TTSIFPDVSADEIPYYEKQTIGYSLGLGYRFWIYYSVGTIWSHSFKSVLNPSGNASEE
                     VMRLEEIGLQEKRKVTFYTSYNSTDDNLNPTKGITAYMGLSMVGGALLWGDDHFVKFS
                     PKIEYFYSPFTLPYLKDYPCVIQLRANAHFMMPPIGDSLVERSQTQNENPWIEAEDRL
                     FVGGPETLRIWKYYDDDLPLSWQNRLFHQITYGSEFRIPVHRQYFWLAFFFDAGALYS
                     DKYWDEYSEYSDDIYSDKENGYLVDIRDIHTVDPLKYFRYSYGFGFKVQIPMLPMRFW
                     FGKKAVWEGLSEGGLKPLEGIEFQLQLGDIRY"
     gene            complement(19237..19647)
                     /locus_tag="P5123_00485"
     CDS             complement(19237..19647)
                     /locus_tag="P5123_00485"
                     /inference="COORDINATES: protein motif:HMM:NF014525.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biopolymer transporter ExbD"
                     /protein_id="HRX14769.1"
                     /translation="MRKVRLIPRHSPKSGIDLTALIDLVFLLVVFFMVTSSMGKVSSI
                     NVTLPYAKNSGESDSSVSVLTVDSQNRVYLNDMLLDSDNLDEFFVKEAEKLKNDTLFI
                     RGDKSADYGVIVKIMDSLSSAGINKFTFSTSTQK"
     gene            complement(19644..20276)
                     /locus_tag="P5123_00490"
     CDS             complement(19644..20276)
                     /locus_tag="P5123_00490"
                     /inference="COORDINATES: protein motif:HMM:NF013760.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_process: GO:0015031 - protein transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MotA/TolQ/ExbB proton channel family protein"
                     /protein_id="HRX14770.1"
                     /translation="MDLVDWLMESGIILNLLISIPTWIMLLPIGLCSLLLLAVFIERM
                     WSYRNYSGTMALSVSSAVMLFNGGQVKDAIAVLKKDTSSISQIFLTMVEVKSVKEREK
                     LVDESVHVLASKIERFTGLISTISTVSPMFGLLGTVTGMMKSFSALAKTSQQAQTLLA
                     SGIAEALVTTATGLLVAIPAVVFYNYMVSRSSVLLKNIEIALNSIMDDGK"
     gene            complement(20305..21876)
                     /locus_tag="P5123_00495"
     CDS             complement(20305..21876)
                     /locus_tag="P5123_00495"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRX14771.1"
                     /translation="MLNSIFAKTFISRLLSFVVFVFLLAFPVCAQSYGDPVDELLFSS
                     EDPEEDTDDGKTGSAKEEIPAKFEKANLDMHVVYGQYNNMLSVINLSQERRGFAYILN
                     SNYKRSNDYGYKDTVYNNSSFFENSLEFSGNLSFDDSFSSTLTVGVENDSRGMFDKID
                     YSREEKDKFTIYSKNIRKFSQNYEAYIVFDAAFYKHRLERRDEEAESIRNYLTRYAGE
                     VGGEYIWSSSNRISYMVQGSQYNYSETGIGDDRFIHGQIVDDFKITGFFGANVGLNTA
                     WSKDSGLFAWDAQGIPLPLMPMAGITFFGNDYYTVYSNYKYTLESFRPEEYYFSLNYI
                     SPTYNLPPAKVHYADLQLDLMMPGAFSVKSVLSYEKSNNFYNYLPSEVSENLLTAHAV
                     PASKWSFTTDLDMNLIGKKVRFLGSYHLKYFQAEENITYQPEVTYIMGLRYDGSLLNV
                     DWTNHFLGEVYVNPETGEKLDNATVGYLDIQFKATESFYAYLRLENLYNEMYYIRDGY
                     PESGLTILFGLRVIL"
     gene            complement(21887..22207)
                     /gene="secG"
                     /locus_tag="P5123_00500"
     CDS             complement(21887..22207)
                     /gene="secG"
                     /locus_tag="P5123_00500"
                     /inference="COORDINATES: protein motif:HMM:TIGR00810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecG"
                     /protein_id="HRX14772.1"
                     /translation="MGILTTILTAVFVIVCVLAILLILLQSDKSSGMGIIGGSSQSTF
                     GSSTADVVTKITGFLVALFLVIALTLGGLYTYSKSASRKALEESSVSSSESSTAGTDG
                     SERE"
     gene            complement(22299..23051)
                     /gene="tpiA"
                     /locus_tag="P5123_00505"
     CDS             complement(22299..23051)
                     /gene="tpiA"
                     /locus_tag="P5123_00505"
                     /EC_number="5.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010258377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004807 - triose-phosphate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="triose-phosphate isomerase"
                     /protein_id="HRX14773.1"
                     /translation="MSRRVIISGNWKMYNTTAEAKELVEGLLAGAGDLGSKEMLIFPP
                     SIHLKEVVSMCSGSKIQVGAQNMYFKEEGAFTGEISPKMVKDSGAEYILIAHSERRHV
                     FGETDKLINDKVKAAFQFDLKPVLCIGELLEDYEAGKSGEVCRNQLVEDLKDISADQM
                     KSMIIAYEPVWAIGTGKVATPEIAESIHKTVREVLKELYSEEIAETVPVLYGGSVKPD
                     NAAGLLSQPNIDGVLVGGACLKADSFLGIAKA"
     gene            23476..24300
                     /gene="lgt"
                     /locus_tag="P5123_00510"
     CDS             23476..24300
                     /gene="lgt"
                     /locus_tag="P5123_00510"
                     /EC_number="2.5.1.145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011416157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008961 -
                     phosphatidylglycerol-prolipoprotein diacylglyceryl
                     transferase activity [Evidence IEA];
                     GO_process: GO:0009249 - protein lipoylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prolipoprotein diacylglyceryl transferase"
                     /protein_id="HRX14774.1"
                     /translation="MWQNLPSHIDPVIISIGSFSIHYYGLMYIVAISLSYFLTHYRIK
                     HENLSYNKEDIESLYVWGVIGMLLGARLGYVLFYDFTYFMTHPLQILLPFKFEGGFTF
                     TGFRGMSYHGGALGAALSAYLYIRRHKFDFFELADLVTPTLAIGYTFGRIGNFINGEL
                     YGRVTDSAIGMYFPRDGSKSLRHPSQLYEAFFEGIVLFTILWLIKKNKHFKYMMLPLY
                     FIGYGFFRFFIEFFREPDAHLGFIAGPFSMGQILCILMMIGGLLFALIIKKFYKKS"
     gene            24379..25215
                     /locus_tag="P5123_00515"
     CDS             24379..25215
                     /locus_tag="P5123_00515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013245087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HRX14775.1"
                     /translation="MKRKIIKINEDLCNGCGQCIPDCPEGALKLIDGKARLVSDLFCD
                     GLGACIGHCPLNAISVEEREAEPYNEALVMKEQIIPKGINVIREHLAHLHNHAETAFL
                     NQAIEILKQQGIAVPDYIEMQPKGCPGCSEHDLSYKDKDKATEQTEAIKQKSELRQWP
                     IQLHLINPSASYFDNCDLLISADCVPYSFGSFHQDFIKDKKVVTLCPKLDHSNDRYID
                     KLTELFKNNEIHSVNVVIMEVPCCGGTSYITQEAIKNSGKNIIYKETVIGIDGSILAE
                     KG"
     gene            25235..25642
                     /locus_tag="P5123_00520"
     CDS             25235..25642
                     /locus_tag="P5123_00520"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRX14776.1"
                     /translation="MDKKQIIEKLKSEHREIVHLLTNAYSGSGIATSSWKDAVIQAKK
                     LFEDHLDHEDQTIYNDEFFSEVFGGTYNKTAEKFQNEMKDITVFVENFFRKYKETTDH
                     KDFNSDYAKLVNALEKRITAEEKILFKEFEEAS"
     gene            complement(25671..26516)
                     /locus_tag="P5123_00525"
     CDS             complement(25671..26516)
                     /locus_tag="P5123_00525"
                     /inference="COORDINATES: protein motif:HMM:NF038196.1"
                     /note="N-terminal region resembles flavodoxins. C-terminal
                     ferrodoxin region binds two 4Fe-4S clusters; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EFR1 family ferrodoxin"
                     /protein_id="HRX14777.1"
                     /translation="MKNIDIIFFEGDGNSSRVSKLLSASLKNSKTRIIDAATLLKTGG
                     YLDSSSALIGLVFPLYLGSPPSTVKMFLDQSRNLTSKHFFIVVLNPIPFKVFSQSASV
                     VTKCIDKKKGNVVALFNIVIPFSGVPDYSAEFLNKLENCVLESVSRISNIIEKARSAP
                     LTLTIFGRILTIMCSFMYKILKIYGLKLFVRSSCDSCGLCIRGCPVSAIEMDRGIKFK
                     NHCVLCMRCVNTCPQEAIGTKRLTRFFKEPRIESANSFDNKKSHKITRLLENLSKAYF
                     ENDQY"
     gene            complement(26513..28540)
                     /locus_tag="P5123_00530"
     CDS             complement(26513..28540)
                     /locus_tag="P5123_00530"
                     /inference="COORDINATES: protein motif:HMM:NF014774.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(Fe-S)-binding protein"
                     /protein_id="HRX14778.1"
                     /translation="MENFQIPDYEILPSFLFRVGSLSSFMPLLIIIGILTVLAINFIE
                     YRYRSSLWKRSNLKGGESKFSSRFINATKLLFFGKATGTRIFDRIYRILLFAAVVTLI
                     LLYLALMLDVLLIKPVMNTTFIQGYYYYLWSIVVNCAAIVLFFLTIVSIYLRLIVRPA
                     YLRTNKTDMLFLFGMMLFFVALIINDALRIAIMDRPVYESGFFAAYAVSWFFKNSDPV
                     LLENYYIAGWTISLLLAVILILLSSIKRSFFSIYLPIHLLNTKPVSLQQKNFYNPKEI
                     IPLKKSGFGQNRLSDFSSDLLRSTDACTECGKCDEKCPAMIAELPLSPKAVIGKLNLL
                     LEKKEAQLSDYINADELFSCFDCGLCTEVCPSGVDPLSVITGLKRHYVLEKGQIPPPL
                     GQLFRTMNHTSASVDNCYNAKSLNDSELPYVQDDIDFDYLLFAGCNYSFDPDKYKNLK
                     FLIKLFLHHGIKVAVLGKEECCCGDAALRGGETALFEKMGKINLRVFEKYKIKKIITV
                     CAHGYHTLKNEYPILAQSEKLPFPEVFHYTEIIRKLIEEEILVFKKSINKNIAFHDPC
                     YLGRFNDMYKTHRVILDSIPGVHITEMRRSGPLSVCCGASGAVNNFEKRKAYKLGEFR
                     AMDAYSNGAHLVTTACPFCYDSISEGVKDINQQGIDVCDIIALLSMSIDEI"
     gene            28744..28817
                     /locus_tag="P5123_00535"
     tRNA            28744..28817
                     /locus_tag="P5123_00535"
                     /product="tRNA-Asp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:28778..28780,aa:Asp,seq:gtc)
     gene            29055..29801
                     /locus_tag="P5123_00540"
     CDS             29055..29801
                     /locus_tag="P5123_00540"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRX14779.1"
                     /translation="MKKIFYMLLISSVVVCISTNVVSKNGKIQSVVSKGTYVPADPIK
                     RALLPKMPVPASYEDYAILQSIDETTNIVIGKFSQGIRRITWISDNNNDGKVDTLIHY
                     FTDDNNFHVVTSPEKIYSTEAFNELKLDIINGSQKKIASNKEGINFYKVLMDKHPEMI
                     TVAKDKKGYRINLIDPDDRGRVRATLSCSNNGINGNDLVLQLNYRHRGNAMLSPVIVY
                     GVYCSNSKDPVVKEAVSDILDYIQKRYPTI"
     gene            complement(29852..30727)
                     /locus_tag="P5123_00545"
     CDS             complement(29852..30727)
                     /locus_tag="P5123_00545"
                     /inference="COORDINATES: protein motif:HMM:NF015637.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MYG1 family protein"
                     /protein_id="HRX14780.1"
                     /translation="MDIKSIAVHDGVFHADDIFAAAIIRMIWPEVKIIRSRNMDVLQG
                     ADLRIDVGGKNCPSSGDYDHHLENGAGTRSNGIPYASCGLIWKEFGMRLAKTRFRFEH
                     IDRKIIQSIDATDCGYSFGEENNSFAHFTISDIFDSFNPSWFKEDNSHDEIFEKAVVI
                     AIQILKNELEKAEGFELSRDYIYAAIDQSVNPFYIVLDKYVPWQHIIINETEFIYVIF
                     PSTTGDWRVRAVPRQLSSFESRKPLPKKWAGKREEELARITGVKDATFCHPARFIAGA
                     QTLEGALMLAEKALE"
     gene            complement(30866..31573)
                     /locus_tag="P5123_00550"
     CDS             complement(30866..31573)
                     /locus_tag="P5123_00550"
                     /EC_number="2.1.1.193"
                     /inference="COORDINATES: protein motif:HMM:NF008700.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (uracil(1498)-N(3))-methyltransferase"
                     /protein_id="HRX14781.1"
                     /translation="MNILLFSDSDQYDDSLIKLDGRRHKHIRHVLKAVKGDNLRVGQI
                     GGKTGRGIIEFINEEYVLIKPEIFENPPEKIPLTLIISMSRPKSMKKVIRYGTAMGIS
                     SFVIINSWKADKSYWQSPFFLEQNIKEEMLMALEQSGDTCYPEISFYRLFKPFVEDVL
                     PKLVKKICLVAHPYAEKPAPSSINCPSVVAIGPEGGFIPYEIEKFEEAGFFSVNMGPR
                     ILRVENAVAAITARLFT"
     gene            complement(31579..32433)
                     /locus_tag="P5123_00555"
     CDS             complement(31579..32433)
                     /locus_tag="P5123_00555"
                     /inference="COORDINATES: protein motif:HMM:NF019844.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0070064 - proline-rich region binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SH3 domain-containing protein"
                     /protein_id="HRX14782.1"
                     /translation="MNIKYVILFFLLVVAGCSKKTEENVVPGERVGPVSAKISETSLI
                     EEVGEENIRRKQIPAGEGEFENGHVLYENSNDEMEILWSGQYEKPVTTVILRKKNSKW
                     KTAEGITVGMTLEELEKINGKPVIFLGFDWDYGGYVMSFNGGVLEKYKGLLNIRIYYT
                     QETDTESIDFNEYSQVIGDREIVSDNPVLKKMKIEIIEMRINLISGNGEDITGVVKAQ
                     NGLVLRSKPSTDGEKITAIPDGTTIKITSLDGPEDEIAGFKAKWIEVEYQGQSGWVYG
                     AFVEYINQ"
     gene            32873..33439
                     /locus_tag="P5123_00560"
     CDS             32873..33439
                     /locus_tag="P5123_00560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010254130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="manganese efflux pump MntP family protein"
                     /protein_id="HRX14783.1"
                     /translation="MDIFTLLIIASALSIDALSVSIANGIIIPQLKTRQAIYISFSFG
                     FFQSIMPVIGWFVGKTFAEYIQVYDHWIAFALLSSVGIKMIHEAYTQSENKTCKNCLQ
                     PKILLLMSLATSIDALAIGISFSILKTDILFPAIVIGVVTFINCFAGITIAHKIGSFF
                     GKKMEYAGGIILIAIGVKIVIEHICLCI"
     gene            complement(33450..35156)
                     /locus_tag="P5123_00565"
     CDS             complement(33450..35156)
                     /locus_tag="P5123_00565"
                     /inference="COORDINATES: protein motif:HMM:NF019874.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cache domain-containing protein"
                     /protein_id="HRX14784.1"
                     /translation="MVKRLILVLILTSVVLLLTLTAYTVISSYGQYKNDSRIIIENSK
                     KNQFKRLKDHSDLSLYSINYQIEHEEKQIKDRLSSEINMLYDRLDSLYRKNGKGDRDS
                     LLLLFKKETASLGKRFYAVSYKGTALIYPENLEFEGRDISFLKDDNGKQIIIEELEAI
                     NKNKSAFVFSHWENSKKNGMVMIKKATCLMRFDPLDLYIGLSDIVPDVDKAGIAEAIR
                     RAEYQSRKAGYGFLSIDYNGNNLVYPDGIYKERNISDIKDLKGNYITPLILESIGKTD
                     GLFIESSRKFENETIKDTIMYCHDIPALGAVIALETVIEISEANRAQIESLNSRALFR
                     VLIIIFIVVSIILISASVLHTVYKTSEVDVTPLVDNCKQPIKQMKRLDPDKYYLPEHK
                     NCIYAVNKLLDELEKKELAFDQLMLFDKLTGLLNEKSFYIDLERELLRYKQFKSGFSL
                     ILLDGDDFKQKTSDFDAKQGSNLLVLIKESISKEIRDIDSAARIKFDTFAVLLPRTSR
                     LQAKLVADRIRRNIENATQLSFSAGVVSVRLGDGSGDAMDRAMRALSRAKQDGKNRTV
                     MI"
     gene            complement(35349..35873)
                     /locus_tag="P5123_00570"
     CDS             complement(35349..35873)
                     /locus_tag="P5123_00570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007784067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydratase small subunit"
                     /protein_id="HRX14785.1"
                     /translation="MKSFGGNILFLDRSDINTDEIIPAKYLTEITKTALKPFLLEDLI
                     LEGFDPAVDINGKQVIVTRANFGCGSSREHAPWALEVNGIYVVIAENFARIFRQNMYN
                     CGMCAIELKTETIDHLFNIYKGISASVDIDLDRGLLTFSGGGKVETVEFQMAPFDLEL
                     IKAGGWVEYADSKY"
     gene            complement(35884..37170)
                     /locus_tag="P5123_00575"
     CDS             complement(35884..37170)
                     /locus_tag="P5123_00575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008515839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydratase large subunit"
                     /protein_id="HRX14786.1"
                     /translation="MGKTIAQKIFDSHLVDMPYEGVNVLRLDAVFCHEITTPIAITDL
                     EARGKDRVFDPSKIKAVIDHVTPAKDSKTAEQGKILRDWALRHKIKDFFDIGRNGVCH
                     AIFPEKGFVRPGYTVIMGDSHTCTHGAFGAFAAGIGTTDLEVGILKGVCAFKAPKTFK
                     FVLKGNLKEGVYAKDVILYIISQLGVNGATNRVMEFTGPVIDSMSMESRMTLCNMAIE
                     AGGTVGICYPDMVTVDYLWPFINNEFENKEAALKEYSEWISDSDAEYEKVIEYDLSSL
                     DPKATFGYKPDQVKSISEMEGTVVDQVYIGSCTNGRIEDLREAAKVLKDKKIADSVRG
                     ILSPATPAIYKQAMDEGILEIFFEAGFCITNSTCGACLGMSNGVLAEGEVCASTTNRN
                     FSGRMGKGGTVHLMSPASAAATAIAGKITNSPLYKG"
     gene            complement(37213..37770)
                     /locus_tag="P5123_00580"
     CDS             complement(37213..37770)
                     /locus_tag="P5123_00580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013757887.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HRX14787.1"
                     /translation="MKYKFGEKIREIREKKKITMKEVAEKASVSESLMSQIERNKISP
                     AIDTLLRIADVLDIDLEYLFSDYKKNRKVNIVRSQERDEYRVKDVFFEQLCRTPGNSE
                     HGIEAYIMKISVGGESKSEEYGHSGKELGVIIEGSGEFKIGSESYILNEGDSISFDSD
                     IPHRLINKGQKHLKAFWVITPPRMF"
     gene            37978..38913
                     /locus_tag="P5123_00585"
     CDS             37978..38913
                     /locus_tag="P5123_00585"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRX14788.1"
                     /translation="MKIFYILLLTLLFLMPSACKKDKQEHTKSSGKESEIYYNTLDRA
                     TKAFEQENYKETEKLIKRAINLEPGMPMAYYMYGMLYVHSNDFLKAGQNFEMALKRST
                     LSEKKRLIDEIDSMTGFIADDDDVNLLEKATNLLDKGNYKQSLLIVEKLLTKYPYNPD
                     VLYLCGTNGIKKGNKDASLHLEKAIKVHPAHQLSFKSLMDYYKETRSNKKLTQLYETY
                     IDYFGESADICTEYAIHVYNCEDTEKALILLDQNIDKFPDYHINHFFAGKIHYSLNKN
                     DLGLKHLSAFIEKSKGTDTDAKLVKEAEQMLIKLK"
     gene            38961..39404
                     /locus_tag="P5123_00590"
     CDS             38961..39404
                     /locus_tag="P5123_00590"
                     /inference="COORDINATES: protein motif:HMM:NF014711.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="divergent PAP2 family protein"
                     /protein_id="HRX14789.1"
                     /translation="MLFELISNKPLVNSIIAFITSQILKSIYFYFSGAKKQLHEFFGT
                     GGMPSSHSATVSALSTTIGITSGWGSVSFAISCVFSSIVIYDAATLRRAAGNNAKKIN
                     EITTKLTESLGPQTIKLKTDIGHTVPEVVAGIALGTLLSVLSFMF"
     gene            complement(39405..40901)
                     /locus_tag="P5123_00595"
     CDS             complement(39405..40901)
                     /locus_tag="P5123_00595"
                     /inference="COORDINATES: protein motif:HMM:NF012245.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein"
                     /protein_id="HRX14790.1"
                     /translation="MYENDELLAIVGVDLNLESVKELVFNTVLFESGYLFLLDDKGDI
                     ILHNKGPMVTPIGETEKISDGDLTEVVRQNSKESVISVESVDGDILLLKKLKNGWTMG
                     GKIRMDEVTASHSKVRIFISLVMVGVIALIIGASFFLQKFIKAKTGNILTVLDYVRSG
                     NFTKKITDVSKSEIGLLNDGINNMIDEIRELALNVSTVSSSVADTAETLDGSMIQTNS
                     IFNNIQSSVLTINDDIMAQATATEEIMASMDNFRDNFKHLNVISNVVAKKANDAVNKN
                     KDWLKNIEILGSTSKKRISSTEDVEQSISKLGQRVFEINDIVTIISDIVEQTNLLALN
                     ASIEAARAGDAGKGFAVVAEEVRSLAEQSQREASSIQQIVKNINLEIETTTEHLNYLR
                     ENTTIEKEALQKADILFKDIYGTFDIIITQINEMNLKIEDMDRIQESVAAGVAEITES
                     ADQNAVSVEKIAVSVKEEGAIINKISESGVELKKLSTQLRTLIGKFVI"
     gene            complement(40914..41531)
                     /locus_tag="P5123_00600"
     CDS             complement(40914..41531)
                     /locus_tag="P5123_00600"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRX14791.1"
                     /translation="MFFGKKSNKGSVKTTKRYLSISHIITGLLIIVSVISAGSSVLVI
                     SAYLFRNLTENAVKSTETILEKVSQTNETELVLVSSMAQSLYLDFKEELGRTSRSERE
                     IDYYDNLLESYSNEIEWVLLSSPDFVYSAYIYLDMDYCGSPYNVQFSRSHDGTIKRQP
                     MVSASIYFLVTGEADHRNWYNTVKYETKKDTWIRGSYDRNEVLMT"
     gene            complement(41950..>42493)
                     /locus_tag="P5123_00605"
     CDS             complement(41950..>42493)
                     /locus_tag="P5123_00605"
                     /EC_number="4.1.1.32"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013314927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004611 - phosphoenolpyruvate
                     carboxykinase activity [Evidence IEA];
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="phosphoenolpyruvate carboxykinase
                     domain-containing protein"
                     /protein_id="HRX14792.1"
                     /translation="TEVGATGVNRQPWANAPFIPGALGDYMDAQFKFFNNAELKEKPI
                     MAGLNYFLTEGSRGGEGKKLLGEKKDVKVWLGWLELRANGDVDAIETPIGFIPLYEDL
                     KKLFAGIDKPYSEELYVKHFSFYVDNMVARLDLQKDAWAKEEKVPSRIFDIYEDQKKG
                     LLALKEKFGAVVTPEQLAKA"
BASE COUNT        13953 a         8599 c         6719 g        13222 t
ORIGIN      
        1 atcaattgtt ttcatgtgca ccatattaat tgcacaagat aatcaaaata gcaaccaaca
       61 tgacgaagcg aatccatatt ggcccatgaa tgttattttt acaaatgcca caactgcttc
      121 ttttgctgac ccgtataatg aagaaaaatc aaaagtagca aaagcaatcg aagataccaa
      181 caataagtta atggaaaaaa ttgactctaa aagagatgaa ttcgaaactc tatttcttgc
      241 taagaaaaaa gaccgtaaaa aaattattca atgcacaaaa gagctttatg ccctccaaat
      301 tgatctcgaa cttgccaata tggaggccag gaaaaaaatg gatgatattg accggaaaat
      361 ggtggatgag ctaaccggta aagttaacat ctggacagaa gaaatgctaa aagatgaatc
      421 taaatacaaa cggtttatta atgaattgaa accggactaa tcatatatct caggagccga
      481 ttatgcaaaa atacattgat aaaattaata ccctaattga atcctttcca tacataaaga
      541 agttttctgg aaaaaccttc gttataaagt atggcggtaa cgccatggtc gaagacaaac
      601 taaaacgcgc attcgctctt gatatggttc ttttgaaata tgtaggcatt cgccctgtta
      661 tcgttcacgg tggcggcccc caaattgcaa acctgctttc ccgtgttggt aaagagtctg
      721 tctttcaaaa tggattacgt gttacagatt cagagacaat ggatattgcc gaaatggtac
      781 ttgttggcca ggttaataaa gagattgtca acatgattaa catggagggt ggacgtgctg
      841 ttggtctttc aggaaaagac gcaaatctga tacttgccga caagatttat catcaggatg
      901 gagaaaatca agttgatata ggccacgtag gaaccgttcg caaagttaac cctagcataa
      961 tcgaaactct ggataaagaa aattatatac cggtaattgc ccctattggt gtaaacgagc
     1021 aaggacaaac ttttaatatt aacgccgata cggccgccgg gaaaattgct gaagctttaa
     1081 acgccgaaaa actgattctt ttaacagata ttgaaggaat aaaaaaagat gataagctag
     1141 tatcttcggc taccattgat gaaataaagc agatgataag tgataaagta atcaaaggtg
     1201 gcatgattcc taaaacagaa tgctgcatct ctgctatcac aaacggtgta cacaaaactt
     1261 caattatcga cggtcgcgta gagcactctg ttttattgga aatatttacc gattctggta
     1321 tcggttcaga aataatgcta aaacatgaag atatataata gttttaaata taaacgcgga
     1381 ttaacgattt tcgccaccgc gttttttttt attccgtttt taaccgtgct aatttcaaac
     1441 agctgcacta aagagacctc caaatctttt gaaactgaag acaatccttt tacattaatc
     1501 atgaccaatg atacttatat tgcctccgag ctatcaatgc cagtaggcaa acctccgctt
     1561 aaagattact ataatgccca aaactttgga atacgtaata agaattttga taataaatac
     1621 catctgggag aagactggaa cggtattggc ggcggagata ccgatcttgg tgatcctgtg
     1681 tacagcatcg gcaatggtct tgtgattttt gcagcaaacg ccggtccagg atggggcaac
     1741 gttgtaatga ttttacatag actaaaaaat gataaattaa tctgctcact ttacggtcac
     1801 ttgcatgact accatgtaaa aacaggtgat tttgtaaagc accgttttca aataggtaat
     1861 ataggaaatg cggacgggct ctactatgca catcttcatt ttgaattaag agaagatatt
     1921 tcccttggtg tggaaggcgg gtattccaat tcaacaaaag gatatcttaa tcctacagat
     1981 tttataattt cctataataa ccaattttta gaatctccat ttttaataaa agatggatct
     2041 tccgttatcg aaagattttt aactcctgca ggcttccaga gagaactttt tagaaaagga
     2101 agttatgaaa attttattca aaatcttccc ctgaaacctt acggacacaa agtacgctat
     2161 tttaataaaa agatcaaaga accgaaaggt atttatatat ctgttctgga ttacgataca
     2221 ggcgaccgtg atctgcttca atgcgctgac gcagtaatgc ttttaagagc cgaatatttt
     2281 ttcaataaat cggatttttc aaacattcat ttcacatttt taagcgatgg taagccacgt
     2341 ttttacaatt ctttcggagg caagctaaat gattataata attttagaaa gtatatgaac
     2401 tacatttttt cttacgccaa cactgcttct cttataaaag aacttagacc cgtcaagatt
     2461 atggatgtta aaccagggga tgtttttata cagaaaggtt ctccatacgg tcatgcaata
     2521 attgtaattg ataccgctgt ttcagcttcg ggtgaaaaaa tgtttatgct tgctcaaagc
     2581 tttatgccag ctcaagaaat tcagatatta attaatccca cggattcgac gatatctccg
     2641 tggtatagaa ccgattcaaa aaagatcata acgccggaat gggtttttag atcagatgat
     2701 ttaaaacgat ttaataaacc taataattta taaatccttt taaaaagcct ttttttaaaa
     2761 ataattgaca gaagcgtgtg ccgagtctcc catattgtat gtattttagg aattttatat
     2821 tttatcagat agaaaaggag aataacatga aaagactttc acttggtctt gattcaagca
     2881 cccaaagtat gaccggagtg cttattgaca ttgataacgg cgaaatagtt tacagcaaat
     2941 cactcgatta tgcatcggat cctcgattaa actgctacgg aattaatcat aaagagtacc
     3001 tcgttccccc aagagaagca ggtgaagctg atcagccccc attgatgtat attgcctcta
     3061 ttgatgcgct ttttgaagac atgaaaaaag acggcataga catgtcggca attaaggtta
     3121 ttaacacatc aggccaacag catggacacc tttatcttaa taaaaaagca aaatcttatt
     3181 tttcttcact aaataatgcc ggcaatgata gtggagacct tgtttcaata atcggctctt
     3241 cattctcata tcagaccgct cccatttgga aaacatccaa taccgaaaca caagctaacg
     3301 cgatcaggga agccgctggc ggaaaagaca aaatgataat gatttccgga tcagattctc
     3361 cattacgttt tactggtgca gtaatccgcc gtgtcggcga acagtttcca caagtttacc
     3421 agcaaaccga aaaaattcaa cttatatcaa gttttatacc ggctgtttta tgtggcaata
     3481 gtgaagttcc tattgatttt ggtaacgcat gcggtatgtc tctgatgaat tatgaacaac
     3541 gcaactggtc aaatactctt attgacgctg tgtcatcttc tttacctggc ggcaacagtt
     3601 ctttaactgc gaaacttcct gacataacct cccccgactc tattgtaggt tctattgcca
     3661 catattttaa agaaaaatac ggatttaacg agtgtgttat tgcagcggga tcaggcgaca
     3721 acccacagac taaagttctt attaaaggtg acctgcttag ccttggaaca agctttgtaa
     3781 atatggtagc aactgatggt acatcatacg atatgggcgg ttttgctaat gccatgtacg
     3841 acggtgttgg acgaccattt atgtttggtt gtcgaacaaa cggcgcgatg gtatgggatc
     3901 gagttcgtgc aaattacggt ttaacaaaaa aagattataa aaaagcggaa gaagctctaa
     3961 aatcggcacc acctgcaaac ggaattttta tgtggcaagt agataacgag tctttccctc
     4021 catctaaaaa atttgacccg gtaagaattg gttcagactc cggttctctg gaatttgatt
     4081 attctggaat cattgactct tctctcgcgg ccgtttttac ctattcacaa gcattcgcgc
     4141 gtaaaacaag cgagcccctt tatattacag gtggacctac atccagcgcg ggaataatga
     4201 gaagagccgc aggcatttgg gggcgtccag ttgtaactat tggttcagta ggggcaggtc
     4261 ttggtactgc tgtagcaggg gctcaggctc tttccaaaca tacaaaagaa agctttgacg
     4321 ccgagcagct ttccgcgtct gttcttccaa gaggagaggc tatcaaccca attgaagaag
     4381 atgtaaaaaa atataaagaa taccttccta aattcaagga aactttcgaa aaatacgcaa
     4441 tgtaaatatt ttgccctctg caaaaacaga gggcaatttt tattttattc aaatcatatt
     4501 atcgattaat tatagcaacg ctactccagt tagttggtgg tttttttatc agcttgttgc
     4561 accgtttaat gtacattgct gataaataat cccaaggcat ctcttcatga caatataaaa
     4621 agcattttaa agcttcattg aattttccgt cgcgataata ttctatccct ttattgtatt
     4681 cactaaggta acgtttttta tgttttttat ttttttcttt gtctgattca aaatactcgt
     4741 aaacagttac aggctcggtc tctcctttta gtttcacaca atcaacatat cttaaattca
     4801 aatcatcact attatctaaa tctgaataag tgcttttgct tagcaatact ggaacacggt
     4861 acatctgggc tatctgactt attccgaaag ctgtatttac agactttccg gttattgtat
     4921 actgcatacg gctttttgac cccacatttc cagctattac ttctccagta tgcaacccta
     4981 tactataatc aataagaggc aaattctttt ccgatagctc tcggttaagt gtaatcagat
     5041 aatctctcag attatccgca cactctaagg caatatttga atgttttccg tttgagacag
     5101 gtgctccaaa tactgctaaa agagaatccg ctataaactt gttaaccaga ccgcctgttg
     5161 attcaattaa accgctcatc ctgtcaaaga aaatattcaa ataagttatc tggtcttcgg
     5221 ctttaaggtc tgaaaccgta tcggtataat tacaaatatc cacaaccatc aaggtcacaa
     5281 aaactttatc accttcaaga tttaatgaac cttttaaaag aaaatttctt acaacagggt
     5341 ctaccatatc tccaaaagta tcttttataa actctttttc ttttaaagat ataactgagt
     5401 tatttattat ttcagctaaa aatccaacct catctgacga tgtgattaaa tttcttccag
     5461 aatagtcacc tcttaccatt tttgtaataa cattcgtcat agacaatatt agtcctctat
     5521 gatatttcac tatcagcgca tacaaaaccg aagttacaac taaaaatata atataatatg
     5581 ttttaatacc atcaaatgct tctatatttt tactacctat agaaatatta taaatatatt
     5641 gcattaaaat tgctgaagga acaacaatag ttgagataaa cagcataata cttttaaaaa
     5701 ttaagcctgg taaaaaagct gacgaacaac tgtttacatc actattgtca acaaaacgtg
     5761 gaataaagat tcgagcgacc agataatcac atataacaaa agagatagaa gatactccca
     5821 aacatatcaa aattaacgac aaaaacattc cggcaattac aaaatcaaaa gatttattta
     5881 ataccacaaa ataaaccaga taaaacaaag gcaccgccca aacaactaaa agaaaacccg
     5941 cgctaagtaa aggagaataa aaaagccttt tactcctctg tctttttata tctaagcctt
     6001 tttttttggc aaaaggaagc attattaatg aaacagccat aaacaaaaca aacgagacaa
     6061 gtacatatat atgttccatc gatttgagta atggctcaac tatttgatta ttcccgctta
     6121 actgtacgtt tgacatgatc catttaaacc cgattaagcc aaataacaca caagataaca
     6181 aaaagaaacc aattataaag acataaaaaa tccgacaaga tgacagcttg tttttatcgt
     6241 gttccttatt gtattttttt ttcatcaaag tactccgtta tatctaatgg tccaatttag
     6301 ttctgagggt aaagcagcaa ttctccctga ataaaattca aatagtattc tggatcatgc
     6361 gaaagaatcg aaatatcatt aatggtacca tctaatattt tactacaata tacttctcga
     6421 accttttttt tataatgtgc tgattgtatc cacaattgaa gaatcagcga ttcatcaaat
     6481 tcttcaagag tcatctcttt taatgaatta atagcaaatc ctttaaacgg agaaaaaaca
     6541 tcgttcaaac agaacccggt aacagaattt attttttgtg aaacaggttc agatggatta
     6601 attattagat aatcgtcagt tttttgtttc atctgtttac ggctaatatt aacaatatca
     6661 gcgccactat ccaatatttc acgcatcaat accacaggtt cggtaaagtt ttcattcgat
     6721 atatcaagaa aatagacacg ctcaaaatca tgtttaaaag catactccat tacagtaata
     6781 aaagaaccac catatcccag cggactttca tgacgtatat acttaagttt ttcaccagtt
     6841 ttaataagat ccccgatacc atcatccgaa ccatcatcaa ctattagtac atcattaaca
     6901 ccctgaacat aagtatacaa ctccgctatt cttcttggag ttattgttgc ctgattatag
     6961 attggtaatg ctatcaactc cattatttaa actcctgccg agctgtttta ccaactaaaa
     7021 gaactattgt gtaaaccagc aaaatctcta acaataccag tacaaacggt ataaaaaaat
     7081 agattatgga aggatatttt gttaatggat tagagccaaa aatagcttct aaagaaacat
     7141 aaaacggcat atctgtaaaa gcattaatca gccgatatac aatccaggga atcaaaataa
     7201 tcgaaaaaca tataaaaaaa agtcgagccc atttttttcg ttttaaaagc tggagtgaaa
     7261 cccacgctga gacaactcca atagcgaaac cgataaaaat cataattata agatctgaaa
     7321 aaaagaattt gaaaaccata tttattattg taaaccacag atttgacgca aaaccgaaat
     7381 gctctaaaac caaagctatt ccgtatgaca aacatattaa tgagcataaa agcgtgaaaa
     7441 taaaatatat tacagatacc agagttattc ctggtttttt cataagcaac accccatagc
     7501 ataatttatt aattttttta tatctgactt ttttatccag tttatatgta aaatcaagca
     7561 ctttaactgt aaaaaccact actatttaaa attacagctc aagacctaaa agcgggcttc
     7621 ttaatattat tgacaactga aaattagcta atttaatgga ctctattatg agcgaagaaa
     7681 ttacagaaat atataaacac aatatcttca taaaatgccg ctttaaatca agcgcgcgga
     7741 acctttatgg cctaatactt atcgatttta acccttccgg cgctgttgac aattaccagg
     7801 ctattgcttc tactttttct gagatatatg agattaatcc gcaatctggg tgggaagact
     7861 acgaagacca gattgtacaa accaagtgga acggtaaata caacaataat attacccgtg
     7921 actttcaact gcaacttgac tccctttttg atgacacttt tcagcgaact cagcttttcg
     7981 aggccgccaa cgattatgat tacgggtctg ccgaacaaat tctttacgac actgcaagac
     8041 gcatattaca ggacaaaaac ctaaagcttg aactaaacat acaagaagtt tcagctgatg
     8101 aaataacaat gattcgaaat cagcgtgaaa ctgaacgcca gcaaaaatac caacaggata
     8161 aagaatccga attttctatc gaagagggct ctgtagtgtt aaattcatcc cttgtgcttt
     8221 ctccagttaa cggaaaacct ctttatgatt tgaaaattgg cgaaaggata atgataaaac
     8281 ttgatccttc atcatccaag aatcagcaat atatttctct ttataatttg aaaacagatg
     8341 caaatacata tctttctgtt cctgctgaag ttattgacat caaagccgca tcgaaagcgg
     8401 aaccagttca aattttaacc agaattgaag attctgttta cgcaaaagca gtcgaagacg
     8461 aaaggcaggt aaaactaaga atgtttaatg ctgcaacaga ccgctccatt caggttatga
     8521 aaaattcatc aaaaaaagct gtatcaaccc aaactatagc aaactataat cgtcaagatt
     8581 cggttttgaa cgaaaactca agcattacaa tagtattggc tcttgcactt gctcttcttt
     8641 tatttttaat tattattgcc atatatcttc ttttatagaa tcataactta acaggtaata
     8701 aaatggatcg cgataaagac tatatcataa aactttctca tgagaaaaat gtaaaattta
     8761 tcaagctctg gtttacagac attcttggta tgcttaaaag ttttactatt acaattgatg
     8821 aattggaaga tgcattatgc gaaggtatcc gatttgatgg tgcaactctt ggtggattta
     8881 tacgaacaaa cgaacacgag atgatcgctt tgcccgacgc gtctactttt caaatacttc
     8941 catggcgtcc aaacgaagat tccgtagcta ggatgttctg tgatatttat acaagtgata
     9001 tgaaacatta tgaggaagat tcacgttata tattacgtaa aacacttaaa gctgcttcag
     9061 atgccggatt tacattctat accggacctg aaatagaatt tttctttttc aaatcaacag
     9121 agaaacccga gttacttgat aaaggtgggt actttgactt aacacctctt gaccttgcag
     9181 cagattatcg aagaatgact gttttaaccc ttgaaaaaat gggtataaac gtaatttcat
     9241 cacatcatga aggagctcat agtcagcacg agatcgacct tcgtcatgaa gatgcattaa
     9301 caacagctga caatattatg acatttagaa ttgttgccaa ggagattgcc caggtaaaca
     9361 atatttatgc ttcttttatg ccaaagcctt tgtcttacca gaacggttct ggcatgcatc
     9421 ttcatctttc actttttgat aaagacgata acaacgcatt tttttcgaca aaggataaaa
     9481 acaacctatc agacaccgcc aaacatttta tagccggatt actaacatat gttcctgaat
     9541 ttacagccat aactaatcaa tggtcaaact catataaaag atttgtttac ggacacgaag
     9601 cccccacaca tatttcatgg agcaactgtt taaacagtgc attaatacgt ataccagaga
     9661 tgcgtaaaga aaaacccgat tccatgcgaa tagagctaag aaatccggat ccttcatgca
     9721 acccatatct ggtcttttca gctatactcg ccgctggcat ggatggtatc aataataaac
     9781 ttgagctcga acctgcactt gaagatcttc agaataattc gaagatagcc gatccggcca
     9841 aacttgggtg taaaaaactg ccatcccatc tttctgaagc tttgttaaat cttcaaaaaa
     9901 gtgattttat gaaaaaaact ttaggaacaa aagtaataga aaaatttatt gaaaacaagg
     9961 tactggaaat agcggagcaa aacagctatg taacggatta cgatattatg aagtattatc
    10021 cgttacttta attgttgacg tattattacc ctataacaga atagtttaat gaaattcaaa
    10081 gaaattagta attattaacg gtatttttat gaaaataact gttaatactc aaaataacac
    10141 ctatttttta attctggatg gcaagtttaa tattgatcat attgatgttt ttgatgagaa
    10201 ctttcattcc ataataaaaa acaagcccga cgccattgcc ataaaccttc agaatgtgga
    10261 atatatagat tcatctgcgt tagggtcatt aataaaaggc ttaaatcttt gtaaaaaact
    10321 taatattaag ttctatcttt gtgatttaaa agagaacatt aatctcgttt ttaaactatc
    10381 atatctggat aagtttttta atatagtttc ttccagtgaa atcaattaat cgcacacata
    10441 tttgacgact atctgtttac cctaaaaacc cgtttctttc aagcctgtcc aaaaaatctt
    10501 cttttgtaga gtcatttgta accatttttt ctatatattt tgccaagata tctacttcaa
    10561 tattgacctt gttaccagca acggcagagt acaggttagt tttttttata gattccggaa
    10621 tcaggtatat actaaagcct ccttcaaata cggatacaac agtaagagat acaccattaa
    10681 cacaaactga acccttttca acaatataca tcatcagttt agagctacag ccaatacgga
    10741 aatcaattgc gttaatgctt ttattaaccg ttaaaacatt acctgtaccg tctatatgtc
    10801 cttgtacaat atgccctccc attctgctcg aagtagttaa tgccctttcc aggtttacat
    10861 tatcttctgt tttaagattg gaaagatttg ttaaatccag agtcactcga gaaacaaaca
    10921 cagtgaatat atcaccatta atagattcaa ccgtttgaca aaccccattt atagcgatag
    10981 aatcgcctat attagcatca gagattaccg tttcggaacg tattcccaac cttactccat
    11041 ttgactcttt tcttatttcc gatactttac ctatctcttc gactatacct gtaaacatta
    11101 tttaaactcc ccataaacag ccagatcacc gtcaattaac gctaaggcct tgtgatttac
    11161 cgtccttgaa ttctttatca tctcttcccc acttcctttg aaaagcgggt gcccgccacc
    11221 aagaaccttg ggagcaaaaa aatagagata cgaatccggt agcacagctt catgaaaaga
    11281 tgcattcaat cgggcaccac cctcaaccat tgcgaacatt acaccctggt cataaagaat
    11341 ttttaacatt ttttcaataa actttttacc ggaaaaacca atagggataa tcttttctct
    11401 atcaacatac tctggtatat cgcaatcatg atcagactca ctacagaata tcagataatt
    11461 atcatcataa aaaaaagatc tattttcaaa ccgatctggg aagccggcaa gtactcgcaa
    11521 gggagttctt ttttttactt caaaatcatt acttattaat tcagaaaaca gaaaagattc
    11581 attagaatat acatttacct ttttattatc aagatactca tttctaacat ccagtcgtgg
    11641 gtcatctaca atgtaagttc ctttacctac aataactgcg tctacaacag atcgtaaccg
    11701 atgcgacaag gctcgtgaac tttctgaagt aatccattta gaatctttat tatgagaggc
    11761 tgtacatccg tcaagggtca ttgcggcctt atgtactata taaggccgct tttcttccaa
    11821 atatgtttta aatggctcaa gaagctctcg agcaaaagat ccattttttt tatcgataat
    11881 tgtttcaata ccggcatttt ccagctctaa aattccttta ccacaaacca gaggatttgg
    11941 atcaatcgcg gcaatgacta ctttttttat tttggcagcg attattgcca tagtacaggg
    12001 tggagttttt ccaaaatgac agcatggctc aagcgtaaca tacattacag aatcttcagt
    12061 tgattcagta cagctattaa tcgcgttaac ctctgcatga tttccaccaa aagttgaagt
    12121 tgaaccgctt ccgattattt tattattctt aacaattacg gcaccgacag gaggattggg
    12181 agaggttata ccgacactgg agaaggccat ttttaaagcc ttctccataa agagggtatc
    12241 aggattcaaa agattacatc cttacttatg ttttattttg tccataattt ttgtatactt
    12301 taacacaact ggcgatgcaa catatattga agaatatgta ccaacaaata ttccaaaaag
    12361 aagaaccatt gcaaagtcgt taattgtatc gccacccaga agataaaggg acaacaccgc
    12421 aaaaagtgtg gttaaagagg ttagaagtgt tcggcttatt gactggctta ttccaatatt
    12481 gattatatca gcaaatatgg gagaagtttc attctctgca ttttcccgta tacgatcaaa
    12541 tattacaatc gtatcgttaa ccgaataacc gaatatagta agaagtgcag ctattacctg
    12601 aacattgatc tctacattca tcataccaca aaacagcact gccaacacaa ggtcatgcgc
    12661 aagagcgatc attgctccaa gggcgtattt gaactcgaaa cggaaagcca aatagatcat
    12721 cattagaaca acagctataa tgacaagttt aaaggccgac tgtttaaggt aatcaccgat
    12781 agtcgggcct acagaatcat ttgcaagttg aacaactgca ggataatccg ccttgagggt
    12841 attccataat agttcataag tagattccgt attctcagaa ccttcaatca attttgtaga
    12901 tattacaaac tcattgttct catcgttgcc gatagtttga acagatgcgt ttagtccact
    12961 tttttgcaga gtggaacgaa catcggatat tgacactcca ctttcaaatt gagcacgaat
    13021 cttaaccccg cctacaaaat cgatacccca gtttaggcca ccttgaggaa tcgttatcgc
    13081 aagaaacaga gccatcaatc ctgcagagac agcatacgct acatatttaa accttagaaa
    13141 aggtaatatc ttctgcttca aagtaatctc cttattaggt aataattatc actaatacaa
    13201 ttctgtttta caacagttat atacttaatt tctttatact tgtcttcgct gtaataagtt
    13261 caaagataaa tcttgataca tacaacgatg caaacatact acatacaata ccaaaagcaa
    13321 gagttacagc aaagccttta attggaccag taccaacctg agacaagata aatgctgcaa
    13381 ttaacgtagt taagtttgcg tcaaagattg tccagaatgc gcgaccaaat ccgttagaca
    13441 ccgcaacacg aggactcttg ccgtttttaa cttcctcttt tatgcgttca tatataatta
    13501 cattcgcgtc aaccgacata cccacagtca agattaaacc agcaatacca ggtaatgtta
    13561 aagttgcttt gatccacgaa aatatcgcca ataaaaacaa catattcaac aaaagacata
    13621 aattagcaag caatccagaa aacttgtaat atattaacat aaaaaccatt attccggcta
    13681 atccaataac aatagcccaa aagccggcag ttattgaatc ttgaccaagt gacggtccaa
    13741 ctgttcgttc ttcaacaaag ataagctcaa caggaagagc tccctcttca ataatacttg
    13801 caagaatttc actctcttcg taggtaaaat ttccggttat ctgtgccgaa ctgctaacaa
    13861 tccgctcatt tatattcgga gcacttctaa cctgttcatc aataacaata gccattcttt
    13921 tgttaacatt ttcgccggtt accttggcaa acttttcggc accttttgta gttaattcga
    13981 aagacaccgc aggtttttga aactgatcct gggtaacatt agctcgttta atgtcatctc
    14041 cggacatgct tacggtttta ctaagaacca tcgggtaaga tggatagagt tttccatcat
    14101 caggattacg ttcgtattgg taaaaaatct gaaaattttc cggtaaacct gtagctaccg
    14161 caatctcggt tgcgatatca cgcaatttaa aacgatcatt cggtaacttt atattatttg
    14221 ctttatggta attatttact ttttcagtcc attcatcatc gacaatacgg tattcaacct
    14281 gaccggtgtt accgatcgtg ttttttactg tttgcggatc actgacacca ggaagttgta
    14341 attctatagc ctctgttcct ttaggtctta caacagattc agaaacacca aacatattaa
    14401 ttcgactgtt aatcttctca attgcctgag ccgttatccg ttttttatct tcttctttaa
    14461 gaggacgccc gatcttctct tccatcttct tataatcggc ctgaattaca aggttcattc
    14521 caccctgcaa gtcaagtccc aacttaattt tacttgctaa cagatatttt tcagcccagt
    14581 gcgtctcgaa ctccgcttcc tcaacgtaat caaagagcat tttgacctcg ttcattacgg
    14641 catgagtaag cgaatacccc ttcatataaa tcgtattatt tccttcataa tcaaacgtat
    14701 atgaatcttc agaaaaacgg gaagcaagct ctttcttggc tctatccatt tctccggaaa
    14761 caactgtcag ttttaatgtc ttctctgcaa atgtaggtaa aatgatcagt acagataaaa
    14821 ctgtaaccaa cagaattatt ataaatctgc ctttaaaacc cttaaccatg taagcctctt
    14881 aattataaac gacgttttga aggtatcttt ctaaaaaccc ttcttcgttt tttctttgat
    14941 tttgaattgt aaagcacaat aagaatcaac acaagtagta ttattagcac acggggaagc
    15001 cacagtttca ccgctctatt catagtacga aaactaccat cctttttagt gccatcctca
    15061 gttgccacga cttcattttt cgtagcaaca ttgtcacctt tatctggcac ttcttccaca
    15121 gatggttcat caatatccgc gatcatcact tcgcgttttt ccagttcaat atccggaaca
    15181 cgtgaatcgt ataacagtgc atctgttatg tctaaaagaa caagtccgga cgatggagca
    15241 accgtttttg gagcaggcac cggttctttt tttcgcgaag cttcctcttt atcggtcttt
    15301 ggtgttgtca ccacagattc atcagaagaa tcaactaact cttctgatga aatttcctct
    15361 tcagtcgcaa catcgtctgt tgtctggcat atcagtgaag aaaatgacaa cagagctatt
    15421 aatgcaaata tgtagactct tctacaaatc attttattat ttttttgcct tattgcgatc
    15481 atctgcagac ggcggcaaaa ccttattctg tattgctgag cgtataaaat caacttttgc
    15541 gttatctgat attttaactg taacaatatc ttcgttttct cttattccgg ttattactcc
    15601 ctgaatacca cctatagtta aaactttatc tccgacccga agctcgttta tcatacgttt
    15661 acgttctttt tctttcttct gctgtggtcg aataatcaga aaataaaaaa tcacaattat
    15721 taagacaaaa ggcgcgaaac tgatcaatcc accacttgcg ggggctgctt tacccgcctg
    15781 agccattaca gtaaaattca atttattcct ccaacaagat cacacaagta aacattaaaa
    15841 taacgcataa acactattat ttaattaatc aaaaaactac ttccgctaaa ttttaatcag
    15901 actattaatg tcagttttta tgtcaataaa taatttagcg cttatcaagc gatcgcctgt
    15961 tatggcgcaa tctcagctca ttaatgacgg attgagtaat atcaagctgt ttatcggcat
    16021 atacaattgt cgatttttta ttaataacca atttgaatcc gttctcagac gctatatatc
    16081 tcactgcatc ttccactttt aaagcaactg aacgctttat ctcgtcagaa ttattcaata
    16141 gctttattga aactggtttt tcactgtttt gctgatacat tttataatca ggatcatttt
    16201 gtaaaacaaa atgatatagg acttctaaat ccacataggc tgtcgaaaaa gatggagatt
    16261 ctgttttaac taaagcgttg catgaaacaa aaaaaattaa acagaaaaat ttgacaaaaa
    16321 acaaaaccag gggtttcaaa aaaatcctcc agcctactgt tttaacttgt ttcgtttaca
    16381 acaggtaatt ttaaaaatgc aacaattgag aatagcacta tttcttttaa taacggatat
    16441 cacccaattg cagctgaaac tcgattcctt caagcggctt aaggccaccc tcacttaatc
    16501 cttcccaaac agccttttta ccaaaccaga accgcattgg aagcataggt atttgtacct
    16561 taaaaccaaa tccatatgaa taacggaaat actttaaagg atcgaccgta tgaatatccc
    16621 tgatatcgac aaggtaacca ttttctttat cagagtatat atcatcagaa tattctgaat
    16681 actcgtccca atatttatcc gaatagagag caccagcatc aaagaaaaaa gcaagccaaa
    16741 agtattgtct atgaaccggt attctaaatt cagatccgta tgtaatctgg tgaaaaagac
    16801 gattttgcca agacaaaggt aaatcatcat cgtaatattt ccatattctt aaagtttccg
    16861 gtcctccaac aaacaaccgg tcttcagctt ctatccaagg attctcattt tgagtttggc
    16921 tcctttctac caaagaatca cctataggag gcatcataaa atgtgcgtta gctcttaact
    16981 gaataacaca aggataatct ttcaaatacg gaagagtaaa cgggctgtaa aaatattcaa
    17041 tttttggaga aaatttgaca aagtggtcat ctccccataa taaagctcca ccaaccatag
    17101 acaatcccat ataggcggta attcccttag ttggattcaa attatcatcc gtagagttat
    17161 atgatgtata aaatgtaact ttccttttct cttgaagccc tatctcctca agacgcataa
    17221 cctcttctga cgcattgcct gaaggattaa gtacggattt gaaagaatga gaccatatag
    17281 tacccactga ataatatatc cagaaacggt atcccaatcc aagagaataa ccaatagtct
    17341 gtttttcgta atagggtatc tcatcagccg atacatcagg gaaaattgat gtcgttctta
    17401 ttttttgcaa tgaatagaat atcgacaagg tcaacgaaac tggattatcg cctatctgat
    17461 aaaaccatgg atcagtaaac gtaactgtcg cggacttatt atattctccg tattccacct
    17521 taacttcagc tctataccca tatccaaaga ggttattctc tgccacactg gcaaaaatag
    17581 aaaaaccaac atctgaacta aatccgcccc ccaaagaaag tgttcctgta ttctgctctt
    17641 taacaccaac aattatatta actttttcat tactgctacc agggcgaata tctattgtta
    17701 cctcttcaaa ataaccaagt cggtacagaa cttcacgtgt tacctgtatt ttttcagcat
    17761 cgtagagttc tccctcttta aaaaggatct ctcttcttat tacatgctct tttgtcttat
    17821 cattaccact aacaataata ttctcaatat atgattgcgt gccttcagca attgtaaggt
    17881 gataatgaac atattttcga atctcttttc taccatcaat tacaacttca cgctcgataa
    17941 tatttttttc gggcataata cgtgtaaata gataaccttg ctttccatat tcaaaagcaa
    18001 gattataacg atccatctga aaaaaagtat cattacatat tgtatctttt tcaacgtctt
    18061 gtcctactaa tttcaaaacc ttgtcagcaa accttgtcag aggagtctct tcctgatgct
    18121 ttagttcaaa aagtttcatt attcgctcgg ttgaaagaat cgaattgccc tcaaccgtat
    18181 atttttcaaa atagtatcgt tcgccttcgt taattcgaag agttatgaag attccacgtt
    18241 tttccttttt ttcaggatca acccaatcgt aaactacctc ttcatcgata atatccgcgt
    18301 caagataacc ttcctgtcga taaagggtta atattttttg tttatctgcc tcatacgtcg
    18361 caacattaaa cttgctttca gtaaataatg atttttcctt tgtttcgata acatttcgaa
    18421 tcagatcatc tcctagttta tatgctccaa acagggttat cttgtgaaca gcaatatcct
    18481 ctccttcatc tatacgaaag gaaaccgtta cattattatt ctcttcatca tcgacatgtt
    18541 tgattacagt tgtaaccatt acattaaaga gaccctcttc tatatatttt tctcgaatta
    18601 ttttttcagc tacaggaatc tggaattccc tataagactc accaatctta acaggaattt
    18661 cttcagacaa gtcagtttca gacaaggtat ccatacctag aaagtttatc atactaataa
    18721 caggtctttc aattataata aaacggacta caacaccctc tccttgttcg gtaacctcag
    18781 ctcgtacatc aagtagatca gcttttttaa aaagggcatt aatatcatcg gtaattactt
    18841 tggggtctaa cggatatcct ttttcagtca gaacatgagc ttcaaggtca gccttgtcaa
    18901 gattacgtat ggctatatct tcttcttttc cattttctag cttctttttt ccgataaaat
    18961 ctacattgac aatggtttta cctttaaagc gtgaaggcat ttgctcttgc gccataagca
    19021 aaccgccctt aaaaaaaggt atagcggcaa ttatcagaat tatataaaac gatattaggg
    19081 gcttttttat cattagaacc acagaaaacc ttacaaacaa tatttatatc gagttcacac
    19141 acaaaatctt gttacaacac ttctaaaaaa agataacaag gttactaagc ctgatacatt
    19201 tatcaagcca tactgtcaag aatttaagag agaaggctat ttttgagtag aagtagaaaa
    19261 tgtaaacttg ttaatcccgg ccgagcttaa tgaatccata attttaacaa taacaccata
    19321 atcagccgac ttatctccac gaataaacag agtatcattt tttagttttt cggcctcttt
    19381 aacgaaaaat tcgtcaagat tatcactatc aagaagcata tcattaagat atacacgatt
    19441 ttgactatca actgtaagca ctgagaccga tgaatcggac tccccactat tttttgcata
    19501 cggcaaggta acattgattg atgacacctt acccatactt gatgtaacca taaagaaaac
    19561 caccaacaaa aaaaccaaat caatcaacgc agttaaatct ataccgcttt tcgggctatg
    19621 cctaggaatc aacctaacct ttctcatttt ccatcatcca taatcgaatt caaagctatt
    19681 tcaatatttt tcagaagcac tgaggagcgt gaaaccatat agttataaaa taccactgcg
    19741 ggtatagcca ctaacagacc cgtagcagta gtaaccaatg cttccgcaat accacttgca
    19801 agaagagttt gtgcctgttg acttgtcttt gccaaagcgc taaatgattt catcatcccg
    19861 gtaactgtgc ccaaaagtcc aaacattgga gacacggtcg aaattgtcga aattagacct
    19921 gtaaaacgtt caatttttga cgcaagaaca tgaacactct catcaacaag tttttcacgc
    19981 tctttaacgg attttacttc gaccattgtt aaaaatatct gtgatataga agaggtatcc
    20041 ttttttaaaa cagcgatagc atcctttacc tgtccaccat taaagagcat taccgcagaa
    20101 ctaacgctta aagccattgt tccagaatag ttacgatacg accacattct ctcaataaaa
    20161 acggcaagaa gcaacaatga gcatagtcca atgggaagca acattatcca agtgggtatt
    20221 gatatcaata aatttagaat tattccagat tccattagcc aatctaccag atccaattaa
    20281 tatttccctt gtgcagggat gaacttatag tattactctc aaaccaaaaa gaatggtcaa
    20341 acctgactcg ggataaccat cacgaatata gtacatttcg ttataaagat tctcaagcct
    20401 tagataggca taaaaacttt cagtagcctt gaactgaata tcaagatatc cgacagtcgc
    20461 attatccagc ttttcccctg tttccgggtt cacataaact tcacctaaaa aatggttagt
    20521 ccaatcaaca tttaacaagg agccatcata acgcaatccc attatatatg ttacttccgg
    20581 ctgatatgtt atgttttctt ctgcttggaa gtatttaaga tgataactac ccaaaaatct
    20641 tacttttttt ccgattaaat tcatatccaa atcagttgta aaactccatt tagacgcagg
    20701 cacggcatga gctgtaagca aattttcact aacttctgaa ggcaaataat tataaaaatt
    20761 attagatttt tcgtaagaaa gaacactttt tacagaaaaa gcacctggca tcattaaatc
    20821 gagctgcaaa tcagcataat gtacctttgc tgggggtaaa ttataagttg gtgaaatata
    20881 gttcaaagaa aagtagtact cttccggacg gaaagattca agagtatatt tataatttga
    20941 gtatactgta taataatcgt ttccaaaaaa agttattccc gccataggca tcaacggcag
    21001 tggaatacct tgggcgtccc aagcgaaaag acctgagtct ttgctccagg cagtgtttaa
    21061 acctacattt gcaccaaaaa atccagttat cttaaaatca tcaactatct gtccatgaat
    21121 aaatcggtca tcacctatgc cggtttcaga atagttatac tgtgatcctt gaaccatata
    21181 gctaatacgg ttagaagatg accaaatata ttcaccgccg acctcacccg catagcgtgt
    21241 caaataattc cgaattgatt cggcctcttc gtcgcgtctt tctaaacggt gtttgtaaaa
    21301 tgccgcatca aaaactatat atgcttcata attttgagaa aattttcgaa tattttttga
    21361 ataaatcgta aatttatctt tttcttcacg ggaataatct attttatcaa acataccccg
    21421 agagtcattc tcaacaccta cagtcaaggt tgatgaaaaa ctatcgtcaa aagagagatt
    21481 gcccgagaat tccagggagt tttcaaaaaa acttgaatta ttatatactg tatccttata
    21541 tccataatcg tttgagcgtt tataattaga gttcaatata taggcgaaac ctcgcctttc
    21601 ttgtgaaaga ttaattacag aaagcatgtt attatactgt ccgtaaacaa catgcatatc
    21661 tagatttgcc ttttcaaact ttgcagggat ttcttcttta gcagagccag ttttgccatc
    21721 atcagtatct tcttcaggat cttcagacga gaacagaagc tcatctacag gatcaccata
    21781 cgactgtgca caaaccggaa aagccaataa aaaaacaaag acaacaaaag agagcaggcg
    21841 agagataaag gttttagcaa atatgctatt cagcataaaa agccctctac tcccgctcag
    21901 aaccgtccgt accggcagta gaactttctg aagaggaaac agagctttcc tcgagagcct
    21961 tacgggaagc agacttggaa tatgtgtaca atccccccag agtcaatgct attactagaa
    22021 aaagagcaac aagaaagcct gttattttgg ttacaacatc tgcagtagaa gaaccaaagg
    22081 ttgactggct tgaaccacca ataatcccca taccagaaga tttatctgat tgaagaagaa
    22141 taagtaatat agccagaaca cagacaataa cgaaaacagc agtcaaaata gtagttaata
    22201 tacccataaa aaaatccttt aatataaggt tttattctgc acgcagacca aacatgatgt
    22261 cgttcttcgt gcagatgata aattttttct aattagaatc aggccttcgc gatacctaaa
    22321 aacgaatctg cttttaaaca cgcgccaccg acaagtacac cgtctatatt tggttgtgac
    22381 aaaagacccg cagcattatc cggttttact gagccgccat ataaaacagg cactgtttca
    22441 gcaatttctt cagaataaag ttcttttaaa acttcacgaa cagttttatg aatcgattct
    22501 gcgatttcag gagtagcaac ctttccagtg ccaatagccc aaacaggttc atacgcaata
    22561 atcatcgatt tcatctggtc agcactgata tctttcaaat cttcaacaag ttgatttcgg
    22621 caaacttcac ccgatttacc agcttcgtaa tcttctaaaa gctcaccaat acataaaaca
    22681 ggttttaagt caaactgaaa cgcagcttta accttgtcat tgattagttt gtcggtttca
    22741 ccaaaaacat gtcttctttc tgagtgtgca attaaaatat attccgcccc tgaatcttta
    22801 accatttttg gagaaatctc tccagtaaaa gctccctctt ctttaaaata catgttttgg
    22861 gcaccaacct gtattttact accagaacac atagaaacaa cttctttaag atgaatcgaa
    22921 ggaggaaaaa tcagcatctc ttttgaacca agatcacccg caccggcaag aagaccctct
    22981 acaagctctt ttgcttctgc ggttgtatta tacattttcc agtttcctga aataattact
    23041 cttctagaca taatatcacc ttttatagaa taaatttatt aacagaactt ttcttaaatt
    23101 taatttttta gaaaagttca acagtttttt acaaaacagt tcaaagggca atattaatca
    23161 acatattttt tatttttaaa ttattaacct actcaaacat ataatttgca agaccgaaaa
    23221 ttttcagaaa atccagcatg taattatcca tttgatgcat ctcatacata agtctgcata
    23281 tcgccaagcc cataaatgaa gcgtacttgt tattcaatgt tccaaataca ataaagtttc
    23341 gtatttgtca gttgtattta taccaattaa ttatattgac aaggaaatct ttaaaggttg
    23401 taacgtttga ttagtaaagt ttgatattta atgaacttta tagacacatc ttacaccaaa
    23461 attgaggaaa acactatgtg gcaaaattta ccatctcaca ttgatccggt aattatttca
    23521 atcggttcat tttccataca ctattatggc ctgatgtata ttgtggctat ctctctctca
    23581 tattttctta cccattacag aattaaacat gaaaacctat cctacaataa agaggatatc
    23641 gaatctcttt acgtctgggg tgttattgga atgcttcttg gagctaggct cggttatgta
    23701 ctcttctatg atttcacata cttcatgaca catccacttc aaatattact tccttttaaa
    23761 tttgaagggg gatttacttt tacaggattt aggggcatgt cttatcatgg tggtgctctt
    23821 ggagccgcat tgtccgcata tttatatata cgtagacata aatttgattt ttttgaatta
    23881 gcggaccttg ttacaccgac acttgcaatt gggtatacct ttggtcgtat tggaaacttt
    23941 atcaatgggg aactttacgg ccgggtaaca gactcggcta taggaatgta ttttccgcgt
    24001 gatggttcca aaagccttcg tcacccctct caactgtatg aagcattttt cgaaggaata
    24061 gttcttttta cgattctttg gcttataaag aaaaataaac atttcaagta tatgatgtta
    24121 cctctctatt ttataggata cgggtttttt cgctttttta ttgaattctt tcgcgaacca
    24181 gacgctcatc taggtttcat tgccggtcct ttctcgatgg gacaaatatt atgtattctt
    24241 atgatgattg gcgggcttct tttcgcatta ataatcaaga aattttataa aaaatcgtaa
    24301 taaagcctac cgaattgacc tgaatcaatc attttcattg ttgccccgtt taatataatt
    24361 acattaacgg aggcaataat gaaacgaaaa ataataaaaa tcaatgaaga cctttgcaac
    24421 ggttgtggac aatgtattcc tgactgccca gaaggcgcac ttaagcttat agatggcaaa
    24481 gcacggctag taagtgatct tttttgcgat ggacttggcg cctgcatagg ccactgccca
    24541 ttaaacgcaa tcagcgtaga agagcgcgaa gctgagcctt ataatgaagc tctcgttatg
    24601 aaagagcaga taattccaaa agggattaat gtcattagag aacacctagc tcacctgcac
    24661 aatcacgccg aaacagcttt ccttaatcag gctatcgaaa tactaaaaca acaaggcata
    24721 gctgttcccg attatattga aatgcagcca aaagggtgcc caggatgttc tgaacacgat
    24781 ctttcgtata aagacaaaga taaggcaacc gaacaaacag aggcgataaa gcaaaaatct
    24841 gaattacgac aatggccgat acagcttcac cttatcaacc catctgcttc gtatttcgac
    24901 aactgtgacc ttcttatatc ggccgattgt gttccgtatt cttttggttc ttttcatcaa
    24961 gactttatca aagacaagaa agttgtcact ttatgcccta aacttgatca tagcaacgat
    25021 cgttatatcg ataagttaac agaattattc aaaaataacg agatacattc ggttaatgtt
    25081 gttattatgg aagtaccctg ttgcggtggt acaagctata ttactcaaga agccattaaa
    25141 aactcgggga aaaatataat ttataaagaa acagttatcg ggatcgatgg atcaatttta
    25201 gctgaaaaag gatagaacct acaggaggcc caacatggac aagaaacaaa ttattgagaa
    25261 actcaagtcc gaacacagag agatagtgca cctgcttaca aatgcttata gtggcagtgg
    25321 aatagcaact tccagctgga aggatgcggt aattcaggca aaaaaacttt ttgaggatca
    25381 tcttgatcat gaagatcaaa ccatttacaa tgacgagttc ttcagcgaag ttttcggtgg
    25441 cacctataac aaaacagccg aaaagtttca aaatgaaatg aaggatatca ctgtttttgt
    25501 tgaaaacttt tttagaaagt acaaagaaac aactgatcac aaagatttta attcggatta
    25561 tgccaaattg gtaaatgcgc ttgaaaagcg aattacagcc gaagaaaaga ttctttttaa
    25621 agaattcgaa gaagctagct gatggttggg gcgaaaagcc ctaaccaatc ttaatattga
    25681 tcattttcaa aataggcttt tgaaagattc tctaagagcc tggttatttt atgactcttt
    25741 ttattatcaa acgaattagc agactcaata cgcggttctt taaaaaaccg agtcaacctt
    25801 tttgttccta ttgcttcttg aggacaggtg ttaacacatc tcatacaaag cacacaatga
    25861 tttttgaatt ttattcctct gtccatttct atagccgata ctggacatcc tcggatacac
    25921 aaaccacaac tatcacaact gcttctgaca aataacttta ggccatatat tttcagtatt
    25981 ttgtacataa acgaacacat aatggtcaga atgcgaccaa aaatcgttaa ggttaaaggg
    26041 gcagaacgcg ccttctcaat tatattcgaa attcgggata cactctctaa aacgcagttt
    26101 tcaagtttat ttaaaaattc tgctgaatag tccggcactc ccgaaaaagg tatgacgata
    26161 ttaaacaatg caacaacatt accttttttc ttatctatac attttgtaac aacacttgca
    26221 gactgagaaa agactttaaa aggaatgggg ttaagaacaa caataaaaaa atgctttgat
    26281 gttagattac gactctgatc taaaaacatc tttacagtag atggtggcga acctaaatac
    26341 agcggaaaaa caagaccaat tagcgcagat gaactatcta aataaccccc tgttttcaac
    26401 agagttgctg catcaataat gcgagtttta ctgtttttta atgacgcaga aagaagtttt
    26461 gatactcgtg acgagttgcc atccccttca aaaaaaataa tatcaatatt cttcatatct
    26521 catctatgga cattgacaaa agagcaataa tatcacaaac atctattccc tgctgattaa
    26581 tatcctttac tccttcagaa atactatcat aacagaaagg acatgccgta gtcacaagat
    26641 gggctccgtt tgaataagca tccatggctc taaactcacc cagcttataa gcctttcttt
    26701 tctcgaaatt gttaacagct cccgaggcac cacaacagac agataaaggg cccgaccgtc
    26761 tcatctcggt aatatgcaca ccaggaatcg aatccaaaat aacacgatga gttttataca
    26821 tatcattaaa acgacctaaa tagcatggat catgaaatgc aatattttta ttaatacttt
    26881 tcttaaatac aagtatttct tcttcaataa gctttctgat tatttcagta tagtgaaaaa
    26941 cttcgggaaa cggtaatttt tcagattgcg caagaatagg atactcgttt ttcagggtat
    27001 ggtatccatg agcacagacc gttatgattt tttttatttt gtatttttca aaaacacgta
    27061 gattaatctt acccattttt tcgaaaagag ctgtttcacc acccctcaag gccgcgtctc
    27121 cgcaacagca ctcttctttt ccaagaacag caaccttaat accgtgatgt aaaaataatt
    27181 ttattaaaaa tttcaaattt ttatatttat cagggtcaaa tgaatagtta catccagcaa
    27241 ataaaagata atcgaaatca atatcatcct gaacatacgg aagttcacta tcgttcaggc
    27301 tttttgcatt gtagcaatta tcaacagaag cagatgtatg gttcatggtt ctaaaaagtt
    27361 gaccaagcgg agggggaatt tgaccttttt caagaacata gtgacgtttt aaacctgtta
    27421 ttacagataa aggatccact ccagacggac atacttcggt acacaaccca caatcaaagc
    27481 aagagaataa ttcgtcagca tttatataat ctgataattg agcctctttt ttttcgagta
    27541 aaaggttcag cttacctata acagctttgg gagaaagagg cagttcagcg atcattgctg
    27601 gacatttttc atcacatttt ccgcattcgg tacatgcatc agtactgcgt aaaagatcag
    27661 agctaaaatc agacaacctg ttttgcccaa aaccgctttt ttttaacgga attatctctt
    27721 tgggattata aaagttcttt tgttgtaacg aaacaggttt tgtattcaac agatgtatag
    27781 gaagataaat tgaaaagaaa ctccgcttta ttgagcttaa cagaataagg ataacggcta
    27841 aaagcaaact aatagtccaa ccagcgatat aataattttc aagtaacaca ggatcactat
    27901 ttttaaaaaa ccaggataca gcgtatgcag caaaaaaacc actttcataa acgggtctat
    27961 ccataattgc tattcgcagc gcgtcattaa taatcaaagc aacaaaaaac agcatcatgc
    28021 caaacaggaa aagcatatcg gttttgtttg tacgtaaata cgcaggacga actatcaaac
    28081 gcaagtatat gctaacaatg gttaaaaaga acaacactat agcggcacaa ttaacaacaa
    28141 ttgaccataa ataatagtaa tacccctgta taaaagtggt attcattacc ggcttaatca
    28201 gaagaacatc cagcataaga gctaaataca ataatatcag agtaacaaca gcggcaaaaa
    28261 gtagtattct gtaaatacga tcgaagattc gggttccagt tgcttttccg aaaaacagga
    28321 gttttgtcgc atttataaaa cgggatgaga attttgattc acccccctta agatttgatc
    28381 ttttccaaag agaacttcta tacctgtatt caataaagtt tattgccaga acagttaaaa
    28441 tgccaataat tattaataaa ggcataaatg acgaaagaga accaacccga aaaagaaaag
    28501 agggcagtat ttcatagtcg gggatttgga aattttccat attgactcaa gctccagtat
    28561 gcctcccatg gatatatgct ggtcgggtta ccgtatagat caaatttatc ttttgataat
    28621 tgcagttaac cgcttgatgt caacaaaaat tagccgtttg accaattgaa ataattttaa
    28681 aattaatctt ctttttttat tgacaggata gggccaaata ttgtcttatc ttttacgaac
    28741 caaggggtag tagctcagtt ggttagagta catgcctgtc acgcatgggg tcgcgagttc
    28801 gagtctcgtc taccccgctt ggataaagga cgaaaagtta ttacttttcg tccttttttt
    28861 ttgtttaata cttctatagt tttttaatta actagttgaa atttttactg ttttatgata
    28921 tttaataaca ggggtatcac tcaactatta ttgattctgt tcgatttact gctataaatt
    28981 cttttttttc aggttactag tcagcactgc ggtaaatatg taaaagttat taattattaa
    29041 attggagtat taacatgaaa aaaatttttt acatgcttct aatttcatct gtagtagtct
    29101 gcatctctac caatgttgta tcaaaaaacg gaaaaattca atccgttgtg tcaaaaggca
    29161 catacgttcc tgctgatccc ataaaaaggg cattactgcc aaaaatgcct gtcccggctt
    29221 cttacgaaga ctacgccatt ttacaatcca ttgacgaaac aactaatatt gttattggca
    29281 aattttcaca aggtatacga cgtattactt ggatttctga taacaataac gatggaaaag
    29341 ttgatacgtt aattcattat tttactgatg acaataattt tcatgtagtc acaagtcccg
    29401 agaaaattta cagtactgaa gcttttaacg aacttaaact ggatattatt aacggatcac
    29461 aaaagaaaat tgcgtctaac aaagaaggaa ttaactttta taaagttctt atggataaac
    29521 accccgagat gataaccgtt gcgaaagaca aaaaaggtta ccgcattaat cttatagatc
    29581 ctgatgatcg ggggagggta cgcgctactt tatcctgttc aaacaacgga ataaacggaa
    29641 acgatcttgt attgcaactt aattaccgcc atagaggcaa cgcgatgtta agcccggtca
    29701 ttgtttacgg cgtctattgc tccaacagca aagatcctgt tgtaaaagaa gctgtaagtg
    29761 acatactgga ctatattcaa aaacgttacc ctacaatatg aatcaattca aattgatgtt
    29821 tacacaagac cccgcaaacg gggtcttttt cttattcaag cgctttttcg gccaacataa
    29881 gagcaccttc aagagtttgc gctccggcta taaatctcgc tgggtgacaa aatgttgcgt
    29941 cttttacgcc cgttatccga gccaactctt cttccctttt tccggcccat tttttgggaa
    30001 gaggttttct tgattcaaac gaacttaact gcctgggtac agctcttacc cgccaatctc
    30061 ctgtagttga gggaaaaatt acatatataa actctgtctc gttaataata atgtgttgcc
    30121 atggaacata cttgtccaaa acaatataaa atggattcac agattgatca attgcagcat
    30181 atatataatc tctcgatagc tcaaacccct ccgctttttc cagctcattt ttcaaaatct
    30241 gaatagcaat aactacagcc ttttcaaata tctcatcatg actgttatcc tctttaaacc
    30301 atgagggatt aaatgaatca aaaatatctg aaatagtaaa atgcgcaaaa gaattgtttt
    30361 cttcaccaaa actataacca cagtcagtcg catctataga ctggattatt tttctatcaa
    30421 tatgttcaaa tctaaagcgg gttttagcaa gacgcatacc aaactctttc catatcaaac
    30481 cacatgaagc gtaaggaata ccattcgacc ttgtgccggc tccgttttca agatgatggt
    30541 cataatctcc tgaagatgga caattctttc cgccaacatc aattcttaga tcagcgcctt
    30601 gcagaacgtc catgtttcgc gaacgaatta tttttacctc cggccagatc attctgatga
    30661 tcgcggcagc aaatatatcg tccgcatgaa aaactccatc atgtactgca atacttttga
    30721 tatccacata aaactccatt ttttaatatt atggtaatct ttaataatta actttttccg
    30781 cggttttttg agcttgcgag aaaatttgcc acaaaaagcc atataatcgc tggttaaagg
    30841 caatacaatc atctcgaagt ttgcttcagg taaacagtct tgctgtaatt gccgcaaccg
    30901 cgttttcgac ccgcaaaatt cgaggaccca tgtttacaga aaaaaagcca gcttcttcaa
    30961 atttctcaat ttcgtatggt ataaaaccac cctctggtcc aatggcaaca acagaagggc
    31021 aattaattga agatggagcc ggtttttcag cataaggatg agccaccaga catatcttct
    31081 taaccagttt tggcaaaaca tcttcgacaa aaggcttaaa aagcctataa aaagatattt
    31141 ccggatagca agtgtcacca gactgttcaa gcgccataag catctcttct ttaatatttt
    31201 gttcaaggaa aaatgggctt tgccaataac tcttgtccgc cttccagcta ttaataatca
    31261 caaaactgct tatacccatg gctgtaccat acctaattac ctttttcatg gattttggcc
    31321 gactcattga aattattaga gttaaaggga ttttttctgg tgggttttca aaaatttcag
    31381 gcttgattag aacatactct tcattaataa attcgattat tcctctacct gtcttgcctc
    31441 cgatttgtcc tactcgcaag ttatcacctt taacagcttt taaaacatgg cgaatatgct
    31501 tatgtcgtct cccatctaac tttataagag aatcatcata ttgatccgaa tccgaaaata
    31561 gcaaaatatt cattagactt attgatttat atactcaaca aaggcaccgt aaacccatcc
    31621 agattgacct tggtactcta cctcaatcca tttcgcctta aatccggcta tttcgtcttc
    31681 agggccatcc aatgatgtta tcttaatggt tgtaccatca ggtattgctg ttattttttc
    31741 tccatcagta gagggcttac ttctaagaac aagtccattt tgagctttta caacacctgt
    31801 aatgtcttca ccgttaccag aaattagatt aattctcatt tcaattattt caattttcat
    31861 tttctttaaa acaggattat ccgatactat ttccctgtca ccgattacct gtgagtattc
    31921 attaaagtca atagattctg tgtccgtttc ctgtgtgtaa tatattctta tattcaagag
    31981 ccctttatac ttctccagaa ccccaccgtt aaacgacatt acatatccac cgtaatccca
    32041 gtcgaatccc aagaaaatta ccggttttcc atttattttc tccaattctt ctaatgtcat
    32101 cccaacagta atcccttcgg ccgttttcca ctttgaattt ttttttcgta aaattactgt
    32161 tgtaaccggc ttttcatact gtccgctcca taatatttcc atttcatcat tactgttttc
    32221 ataaagaaca tgaccgtttt caaattcacc ttctccggca ggaatctgtt ttcgtcttat
    32281 attttcttca ccaacctctt caataagcga cgtttccgaa attttagcag aaacgggacc
    32341 aaccctttcc cccggcacaa cattttcttc ggtctttttt gaacatccag caacaaccaa
    32401 caaaaaaaac agaataacat attttatatt catttaaact ctccagtata taatgagtaa
    32461 tttagaaata ttgccgaact ccagaagact aaaccaacca tttaacataa taaacaccaa
    32521 ttatcataat aaacaccaat tatcaagttg tcactctaaa aacggccgaa attttaaact
    32581 taataattac cagattttaa ttatattaaa caaattggct caatttactt tttgaatatc
    32641 atttatattc aaaataaaca tcaatcatga atatcttagc atcactgctt ttacattgta
    32701 aatttccccg cgcagaaccg tcaacaccta ttggtttatt ggtaataaca aaatagtgga
    32761 tttatttgac aaataaaact aaatggttat taattaaaaa accagtaatg tattagactt
    32821 gctagcagaa aatcatacca aaaaaatata attcagctca aggtaataga caatggatat
    32881 ctttactctt cttataatcg cttcggccct ttctatagac gcgttaagcg tttcaattgc
    32941 caacggaata attattcccc aactaaaaac aagacaggct atatatatat ctttctcttt
    33001 tggatttttt caatcaatta tgccagttat tggttggttt gttggcaaaa cattcgcgga
    33061 gtatatacaa gtatatgacc attggattgc ttttgcgcta ctttcgtcgg taggaataaa
    33121 aatgattcat gaagcgtata ctcaaagtga aaacaagaca tgcaagaact gtcttcagcc
    33181 aaaaattctt ttattaatgt cattagcaac aagtatcgat gctctggcaa tagggatcag
    33241 tttttcaata ctaaaaacgg atattctttt cccggccatt gttattggag ttgtaacttt
    33301 tataaactgt ttcgcaggaa taactattgc tcacaaaata ggatcatttt ttggaaaaaa
    33361 aatggaatat gctgggggga taatcttaat tgctataggt gttaaaattg taatcgagca
    33421 catctgtctt tgcatctgac ttttctcgat tatatcatta cagtccgatt tttaccatcc
    33481 tgcttagcac gtgacaatgc acgcattgcc ctgtccatag catcaccgga gccatcccct
    33541 aggcgaacag aaacaacacc cgcgctaaaa cttaattgtg tagcgttttc tatattccgg
    33601 cgtattctat cagcaacaag ttttgcctga agacgacttg ttcttggaag taaaactgca
    33661 aaagtatcaa attttattct tgcagcagaa tcgatatccc taatctcttt ggatatggat
    33721 tctttgatca atacaagtag gtttgacccc tgtttggcat caaaatccga tgttttttgc
    33781 ttaaaatcat ctccatcaag aagtattaat gaaaaaccgc ttttaaactg tttataccta
    33841 agtaactccc tttccaaatc tatataaaaa cttttttcat ttagcaaacc tgttaactta
    33901 tcaaatagca tcaattggtc aaaggctaac tctttttttt caagttcatc aagaagttta
    33961 ttaacagcat agatacagtt tttatgctct ggaagatagt atttatcagg atctagcctt
    34021 ttcatctgct taatcggttg cttacaatta tcaactagcg gggttacgtc tacctcgctt
    34081 gttttatata cagtatgcaa cactgaagca cttattaaaa ttatagacac aactataaat
    34141 attataatta atactctaaa cagggctctt gaattaagac tttctatttg agctctattt
    34201 gcctcggaaa tttctataac cgtttctaat gctataacag ctccaagagc cggaatgtca
    34261 tgacagtaca ttattgtatc ttttattgtc tcattttcga attttctgga agattctata
    34321 aacaatccat cggttttccc aatagactca agaattaatg gcgtgatgta atttcctttc
    34381 aaatctttaa tatcacttat atttctctct ttataaatgc catcaggata tacaagatta
    34441 ttaccgttat aatcaataga taaaaaccca taacctgctt ttctcgattg atattcagct
    34501 cttcttatag cctctgcgat tcctgcttta tcgacatccg gaacaatatc gcttaaacct
    34561 atgtacaaat caagagggtc aaagcgcatt aggcaagtcg cctttttgat cattaccatc
    34621 ccattttttt tactattttc ccaatgtgaa aaaacaaaag ccgatttatt tttattgatt
    34681 gcctccagct cttctataat aatctgcttt ccgttatcat cctttaaaaa acttatatca
    34741 cgaccttcaa actccaagtt ttcgggatat atcaaagcag tacccttata cgatacagcg
    34801 taaaaccttt tacctaaaga ggccgtctcc tttttaaaca acaacagcag gctatcacga
    34861 tcccccttcc cgtttttccg ataaagagaa tctagcctgt cataaagcat gtttatctca
    34921 gaagataatc tgtcttttat ttgtttttct tcatgttcta tttgataatt tatagaatag
    34981 agcgacaaat cggaatgatc tttaagtctc ttaaactgat tttttttgct attctcaata
    35041 attattcgtg aatcattttt atattggcca tacgaagata taacagtata ggctgtcaaa
    35101 gtaagaagta aaaccacaga tgttaatata agaaccaaga ttaaacgttt aaccattaga
    35161 attttctgcc tataacatca aaacttaaaa ttatccttac agaatgcttg attatcaatc
    35221 agcctgcaag gataattttt ttaagttaat taacggaaaa ttcctgtttt aaatacaact
    35281 acaaccttgt tcaataccat gccgttttga catgtaacca gatatccgtt gcaatttgca
    35341 gccaaatatc agtatttact gtcagcatat tcgacccaac cacctgcttt tattagctca
    35401 aggtcaaaag gcgccatttg aaattcgact gtttcgacct ttccaccacc agaaaatgtt
    35461 agcaatcctc gatccagatc aatatcaacg ctagccgata ttcccttata aatattgaaa
    35521 aggtgatcaa tagtctcggt cttgagctca atcgcacaca tcccgcaatt atacatattt
    35581 tgcctaaaaa tacgggcaaa attctctgca attacaacat aaatgccgtt aacttcaaga
    35641 gcccaaggag catgttcacg ggacgagcca caaccaaagt tggctctggt aacaataacc
    35701 tgtttgccat ttatatcaac agcagggtca aacccttcaa gaatcaggtc ttcaagaagg
    35761 aaaggcttca atgccgtttt ggtaatctct gtaagatatt ttgctggtat tatttcgtct
    35821 gtattaatat ccgaacgatc cagaaagagt atatttcctc caaaactttt cataattatc
    35881 tccttatcct ttgtacaatg gtgaatttgt tatctttcct gcgattgcgg tagccgccgc
    35941 gctagctgga ctcattagat gaacagttcc gccttttccc atacgtccgc taaaatttcg
    36001 gttagtggta gacgcacata cctccccttc agcaagaact ccattactca taccaagaca
    36061 ggcaccacat gttgaatttg taatacagaa tcctgcttca aaaaatattt ccaaaatacc
    36121 ctcatccatg gcctgtttat aaatagctgg ggtagctgga gacaatattc ctctgacgct
    36181 atcagcaatc tttttatctt tcagaacctt ggctgcttca cgcaaatcct caatacgacc
    36241 gtttgtgcac gacccaatat aaacctggtc cacaactgtt ccttccattt cgctaatact
    36301 ttttacctga tctggcttat aaccaaatgt cgcctttgga tcaagagaag aaaggtcata
    36361 ttcaattacc ttctcatact cggcatcact gtcagaaatc cattcagaat actcttttaa
    36421 agcggcctct ttattttcaa actcgttatt gataaacggc cacaggtaat caactgttac
    36481 catatcagga taacagatac cgacagtgcc gcctgcttca atagccatat tgcatagagt
    36541 catacgagat tccatactca ttgaatcaat taccggccca gtaaactcca tgactctgtt
    36601 tgtcgctccg ttaactccaa gctgagagat tatgtaaaga ataacgtcct tggcatacac
    36661 accctcttta agattacctt ttaaaacaaa tttgaatgtc ttaggagcct tgaacgcaca
    36721 aacccccttc agaatgccca cctctaaatc tgttgttcct attccagccg cgaaagcacc
    36781 aaacgctcca tgagtacagg tatgagaatc tcccataata acggtgtatc cgggtctcac
    36841 aaaacctttt tcaggaaata tagcatgaca gactccgttt cgccctatat cgaaaaaatc
    36901 ctttatttta tgacgaagag cccagtcacg aagaattttt ccctgttcag cggttttaga
    36961 atcttttgct ggggtcacat ggtctataac tgccttgatt tttgaaggat caaaaacacg
    37021 gtctttaccg cgcgcttcta ggtcagttat cgcaatagga gtagtaatct catgacaaaa
    37081 aactgcatca agtcttaata cattgactcc ctcatagggc atatctacca gatgtgaatc
    37141 aaaaattttt tgagcaatgg tttttcccat aattgcctcc taactaaatt aatatgtcat
    37201 cggaacatta cattaaaaca ttcttggcgg tgttatcacc cagaatgcct taagatgctt
    37261 ttgtccttta ttaataagcc tgtgtggtat atccgaatca aaagagatgc tatcgccttc
    37321 gttaagaata taagattcac ttcctatttt gaattcacca ctaccttcaa ttattacacc
    37381 caactctttt cccgaatggc catattcttc tgatttactt tctccaccca ccgatatttt
    37441 cataatgtag gcttcaatac catgttcaga atttccagga gttctacaga gctgttcgaa
    37501 aaagacatcc ttcaccctgt attcatcacg ctcctgagag cgaacaatgt ttaccttacg
    37561 gtttttttta taatcactga aaagatactc gagatcgata tccaaaacat cagcaatacg
    37621 caaaagagta tcgattgccg gcgatatctt atttctctct atctgagaca taagactttc
    37681 actaacagaa gccttctcgg ctacctcttt catagtgatc ttcttttttt ccctgatttc
    37741 ccgaatcttt tcgccaaatt tatatttcat atcgctcttt atatatattt aagatacttt
    37801 agtatatata aactatcgtc aaaattgtca aggaaaaaca ggccattttt tacgatttac
    37861 aacaaaatta gtaatttttc attgttctct attataaact ttacagacaa aaatcaaata
    37921 tcataatgtg tttttaaaaa acagaaacat aaattatttt acataaggct taaaaaaatg
    37981 aaaatcttct acatattact gcttacatta ctattcttga tgccttcagc atgcaagaaa
    38041 gataaacaag aacacacaaa gtcttccggg aaagagtcag aaatatacta taacactctt
    38101 gatcgcgcga caaaagcttt tgagcaggaa aactataaag agacggaaaa actgattaaa
    38161 agggcaatca atctggaacc gggaatgccc atggcgtact acatgtacgg tatgctgtac
    38221 gttcacagca atgattttct taaagccggt cagaattttg aaatggcact taaaagatca
    38281 accctgtccg agaaaaaaag attaattgat gaaattgact ctatgaccgg ttttatagca
    38341 gatgatgatg acgtgaattt attggaaaaa gctacaaact tgttagacaa aggtaattac
    38401 aaacaatctc ttttaattgt cgagaaattg ctgactaaat acccttataa tcccgacgtt
    38461 ttgtaccttt gcggcacaaa tggaatcaaa aaaggcaata aagatgcgtc tttacatctg
    38521 gaaaaagcca tcaaggttca tcctgcgcat caactatctt ttaaatcatt aatggattat
    38581 tacaaagaaa ccagatcgaa taaaaaattg acgcaattat atgaaacata tattgattat
    38641 ttcggtgaat ctgctgatat ttgtacagaa tatgctatac acgtttataa ttgcgaagat
    38701 acagaaaaag cattgattct tctggatcaa aatattgaca aatttcccga ttatcatatt
    38761 aatcattttt ttgcgggtaa aattcactac tctttgaaca agaacgatct cggattaaag
    38821 catctttctg ccttcattga aaaatcgaaa ggaacagata ctgatgcgaa acttgttaaa
    38881 gaagccgagc aaatgctgat aaaattgaaa tagattattt taattcccta agcaaccgct
    38941 aaaaatttct ggagaaaaat atgctttttg aactaatcag caacaaaccc ctggtaaact
    39001 cgattattgc ttttataaca agccagatat taaaatctat ttacttctat ttctcgggcg
    39061 caaaaaaaca gttacatgag ttttttggca ccggtggaat gccctcttca cattcggcaa
    39121 cggtatctgc tctatctact acaataggaa tcacttccgg ctggggaagt gtttcatttg
    39181 caatatcatg cgtgttcagt tcaattgtaa tatacgacgc agccacctta agacgagctg
    39241 ccggaaacaa tgcaaaaaaa ataaatgaaa taaccactaa actaactgaa agtcttgggc
    39301 cacagactat aaaacttaag accgatattg gtcacaccgt acctgaggta gtagccggaa
    39361 tcgcgttagg aactcttttg tcagtattat catttatgtt ttaattatat tacaaatttg
    39421 ccaataagag ttcttaattg cgttgaaagt ttcttaagct ccacaccgct ttcggatatt
    39481 ttgttaatta tcgccccctc ttctttaacc gatactgcta tcttctcgac agaaacggca
    39541 ttttgatcag cagactctgt tatttcggca actcccgctg caacactctc ctgtatacga
    39601 tccatatcct ctatcttcaa attcatctcg ttaatttgag taataataat atcaaaagtt
    39661 ccgtatatat ccttgaataa aatatctgct ttttgcaacg cctctttttc aatagtagta
    39721 ttttcacgca aataatttaa atgctcagtt gtggtttcaa tttcaaggtt tatgtttttt
    39781 acaatctgtt gtatgctcga agcctctcgt tgcgattgct cggccaacga cctgacctct
    39841 tccgcgacaa cagcgaaacc cttaccagcg tcaccagcgc gagcagcttc aatactggca
    39901 tttaatgcta acagatttgt ctgctcaaca atgtccgaaa taattgttac tatatcattt
    39961 atctcaaaga ccctctgtcc caacttactt atggattgtt ccacatcttc ggttgaagat
    40021 attcgcttct tagaagtact tcccaatatc tctatatttt taagccaatc cttattctta
    40081 ttcaccgcat cattggcttt tttagctaca acattgctga ttacgtttaa atgtttaaag
    40141 ttatcacgaa aattatccat cgaggccatt atctcttctg tggcggtagc ctgcgccata
    40201 atatcatcat taattgttag tactgaactc tgtatgttat taaaaatgct attggtttga
    40261 atcatagaac cgtcaagagt ctccgctgta tccgctactg acgaacttac agtacttaca
    40321 tttagcgcaa gttctcttat ctcatcaatc atattgttta ttccgtcgtt caacaaacct
    40381 atctcgctct ttgaaacatc agtaattttt tttgtaaaat taccacttcg cacataatca
    40441 agaacagtta aaatatttcc cgttttagcc tttataaact tttgcagaaa aaaagaagct
    40501 ccgataatca acgcaataac accaaccata acaagcgaaa taaaaatcct taccttggaa
    40561 tgacttgctg ttacttcgtc catgcgtatc ttgccgccca ttgtccatcc gttttttagc
    40621 tttttcaaca gcaaaatatc tccgtcaacc gattcaacag aaattacaga ctctttactg
    40681 ttttgccgca caacctctgt taagtctccg tcactaattt tttctgtttc tccgataggc
    40741 gttaccattg gccctttatt gtgaagaatt atatcccctt tatcatccaa aagaaaaaga
    40801 taaccgcttt caaaaagcac tgtgttaaaa acaagctctt taacagactc caggttaaga
    40861 tcaactccta ctattgcaag aagctcatcg ttctcataca caggaatcag gtattaagtc
    40921 attaaaactt catttctgtc gtaagatcct cttatccagg tatctttttt cgtttcatat
    40981 tttacggtat tataccagtt acgatgatct gcctctcctg ttacaagaaa ataaatagac
    41041 gcagaaacca tgggctgtct cttgattgtg ccatcatgac ttctggaaaa ctgtacatta
    41101 taggggcttc cacaataatc catatcaaga tagatatagg cgctgtatac gaaatcaggg
    41161 ctacttagca atacccattc aatttcattt gaatacgatt caagcagatt gtcataatag
    41221 tctatttctc tttcactacg gctggttcgc ccaagttcct ctttaaaatc cagatataac
    41281 gactgagcca tactgctaac aagaacaagt tctgtttcgt ttgtctgtga caccttttct
    41341 aaaatggttt ctgtactctt cacagcattt tcagtcaagt ttctaaacag atatgctgat
    41401 attacaagca cagaacttcc cgcacttata actgaaacta ttataagcaa cccggtaatt
    41461 atatgagata tcgaaagata tcttttggtt gttttaaccg atcccttatt agattttttt
    41521 ccaaaaaaca tatttcctct taaatttgtt ataagcaaaa ttataaaatt tatgatgaaa
    41581 gcctgacatt ttcgaacctt acatcactat tttattaata gcacctcttt agataatgta
    41641 agaaagtttt tagcgaaaac aaaaaaagat tctgaagttt ccaaattttg acaactttcg
    41701 atatcaggtt ttccaggtta gcagcgtaat tatattttgt attagcttga ttttgagatt
    41761 atgttattac gtgcaactga ttagctgagc ttaagtgaaa cagaataatc acgcaggtaa
    41821 ggtgtttagg atttatatgg gtttataaga ggtaattaac aaaagaaaga ctttagatta
    41881 atatattatc aaggagataa ataaaaaggg cctttcgacc ctttttattt attatattgc
    41941 tgtatctttt tatgcttttg caagttgttc tggtgtaaca acagcaccaa atttttcttt
    42001 aagagcaagc agaccttttt tctgatcttc atagatatca aagattcttg aaggaacttt
    42061 ttcctctttt gcccacgcgt ctttctgaag gtcaagacga gcaaccatgt tatcaacata
    42121 aaaagagaaa tgtttaacat aaagctcttc tgaatatggt ttatcgatac ctgcaaaaag
    42181 tttctttaga tcttcataaa gtggaataaa acctattggt gtttcaatag catcaacatc
    42241 tccgttagca cgaagctcaa gccatccaag ccacacctta acatcttttt tctcaccaag
    42301 aagttttttg ccttcgccgc ctcgagatcc ttcggtcaaa aagtagttca aaccggccat
    42361 aataggcttt tctttaagct cggcattatt aaaaaacttg aattgggcat ccatatagtc
    42421 accaagcgcg cctggaataa acggagcatt tgcccaaggc tgtcggttta caccagttgc
    42481 tccaacctct gtt
//
LOCUS       DAOTGZ010000004        41473 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_3064
            NODE_994_length_41473_cov_5.206335, whole genome shotgun sequence.
ACCESSION   DAOTGZ010000004 DAOTGZ010000000
VERSION     DAOTGZ010000004.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868088
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 41473)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 41473)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:22:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,785
            CDSs (total)                      :: 2,751
            Genes (coding)                    :: 2,732
            CDSs (with protein)               :: 2,732
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 12 of 19
            Pseudo Genes (internal stop)      :: 5 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..41473
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_994_length_41473_cov_5.206335"
                     /isolate="AMR_MDS_3064"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..564)
                     /locus_tag="P5123_00610"
     CDS             complement(<1..564)
                     /locus_tag="P5123_00610"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRX14793.1"
                     /translation="MADYIQLKRGGYLVDTCLGPVQIGVPPETIKDTMIMERGVPSFF
                     VLPATLFDWIKGISVAEIEFPLYFNFFVRKQKTHVICRKEQIVRMREVLQESLFGPEN
                     LDVHVDFETDYPGSKNRYFDLKKEIDYFRSSFSLDDLVTFLPFKNNSISIGKKKSGKN
                     ITINYNESNSTYEIIQNGDLLCCTPSVI"
     gene            complement(733..1668)
                     /locus_tag="P5123_00615"
     CDS             complement(733..1668)
                     /locus_tag="P5123_00615"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRX14794.1"
                     /translation="MKIKLLIVMFVFFCVTTLSSDEGNIFFVCDNVRIVIKEWSGDNF
                     KIGEDEYGYDMFLYPSKKGDYQITTTLYPYNSDKTSNEQLKSIITKQGQELLPSAVEK
                     ELSIEELNKNGKIKGYFYFLTDKNDVSNKEGEYAYLIQGIIRYNYLLGSFTILSNDKK
                     VKTAFLSDIYDLVNDRLEIGETKNFEKIKLNPSEIKKYNLSHIKNFYSNHQALFYNNS
                     NNYRHILPVISEKYTQSTDNNGEQGTVFYYYFDEKLDDGHKSFLTKLFFGQGKPGSKE
                     YPELMLIKDNYLIIFSFPYQSKDGKEIYDIVKKKL"
     gene            complement(1684..2529)
                     /locus_tag="P5123_00620"
     CDS             complement(1684..2529)
                     /locus_tag="P5123_00620"
                     /inference="COORDINATES: protein motif:HMM:NF012955.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HRX14795.1"
                     /translation="MKKITLNDQPFSLKNDGALQLFFVGTGSAFTKFNYQTNLLIIKG
                     DDHLLVDCGTKCPQAFNDLGSSITEVQNFLITHSHADHIGGLEEVALMNKYVTKKKPV
                     MTINETYQYILWEMSLRGGAAYNEENAGDILTFSDFFGINRPKVLQGYQRETLEANVG
                     SINLKIFRTKHIPDNSRDWESSFWSCGVIIDDKVMYTSDTRFDRELVEYFDSRFELEA
                     IFHDCQLFTGGVHASIDELNGFSDNIKRKINLVHYGDNYKDFIQKSADYGLNGFTEPH
                     TYYTF"
     gene            3191..4567
                     /gene="dgt"
                     /locus_tag="P5123_00625"
     CDS             3191..4567
                     /gene="dgt"
                     /locus_tag="P5123_00625"
                     /inference="COORDINATES: protein motif:HMM:TIGR01353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008832 - dGTPase activity [Evidence IEA];
                     GO_process: GO:0015949 - nucleobase-containing small
                     molecule interconversion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dNTP triphosphohydrolase"
                     /protein_id="HRX14796.1"
                     /translation="MMINNEFWESCVVTKRLLSNEKAFDNESRSPFDMDHDRIIYSNF
                     FRRMHDKTQVFPYAPLSNSGQARSRLSHSLEVSCVARSLGNLLGHYLVKNGAKIRPYD
                     IGMIVASACLAHDIGNPPFGHSGEYAIQSWISNNLEFALKDKWEKNDIMNFEGNAQGF
                     RILSRLESCQRPGGLRPTVSTLAAFMKYPCASIDTDKNSNNPGLKKFGFFKADRESFD
                     DVFDSFNIPRQKNCVNAYTRHPFAFLTEAADDICYAIVDLEDAYHLSVIGFDSVYDLL
                     MPIVSSNSSFKDNKEFDKDIRVARMRSAAISVLIDQTIDVFTDNIELFEKYSFSTPLI
                     NKIDLSSSYSVIKNFSFNKIYNSSRVLEIECAGFKTIGGLFDMFVNAVLTDKPVAQEK
                     ISRQLIPSQYFKRHENSIQSNNHNQLIERLSTYERILSITDYICGMTDRYAVELYQRI
                     SGIRLPVY"
     gene            complement(4577..5833)
                     /locus_tag="P5123_00630"
     CDS             complement(4577..5833)
                     /locus_tag="P5123_00630"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009975 - cyclase activity [Evidence IEA];
                     GO_process: GO:0009966 - regulation of signal transduction
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HRX14797.1"
                     /translation="MFKILHIETSTLYQKLISDICHELDIDYVSCSSSKDALAVLSTD
                     NISLIVTAMEIREGSIERFIELINRSYSKVPIVVITGNDTFEDRKKMYDLGIVDYIVK
                     KSSRDEIRDYLKAFAVKSKTQYLTDMYIAVCDDSKVDRKITERIFRINSINRVDFYED
                     PEEMFEAGRTYDIYLVDLILKDSSGHQVIKKLRSDGCKSVIIAISGIDNVKTISHVLS
                     LGADDYITKPYNNDIFMARIRTCARSYSLYKEVKEKTLELEKMAKSDGLTGLLNHMEI
                     FRQLDLEIEKKGRYGDKLSLIMLDLDFFKNINDTYGHLFGDKVLKSVSSIIQKNIRNV
                     DLAGRYGGEEFMIILPKASLKQAVETAERIRDSIEKLAFENTDLNVTISGGVAECIKE
                     TSKELVSIADNLLYKAKESGRNNIQF"
     gene            6154..8223
                     /locus_tag="P5123_00635"
     CDS             6154..8223
                     /locus_tag="P5123_00635"
                     /inference="COORDINATES: protein motif:HMM:NF017775.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein/permease"
                     /protein_id="HRX14798.1"
                     /translation="MKKNRQKKMHNVIHYLKLTHQYWFPSGKLSIPSFTLLLTALICF
                     IASMLYLFLNLIVASGKFIPRLTSFLSSVFSVPLLTVPEDFLSRIAPGLTEWLAGIRG
                     GNLNTFFIASSVVAIVIFLLFRKKIKGRKLPWIILFVLLTLSMMVSGVNVILSYTGRF
                     WQTALQQRDTVSFWRFIFVHLGMYVVAMPIVVFYPWIQGILGIKWRKWLTDKFVGSYM
                     KNRAYYEISNNKEIDNPDQRISEDLNRFTVESLDYLLIILGAIIDLTAFTLILWSISQ
                     KLTGILIAYSFFGTACTIFIGKKLIGLNFEQLRREANFRYGLVHIRDNTESIAFYSGE
                     STEKKNLAVRFKSVVSNYIRLIGWERNLKFFTKYYHYMQVLIPYIIMAPAYFREEIEF
                     GVMYQASFAFAMIIGAFSIIITYFNSITRYVAGIQRLTSFEDVLINSRGIRKIEGQKG
                     SYKIKREKAPLIEVSNLTLKTPDFSKSLARNLSFRIDKGESLLIVGPSGVGKSSLIRT
                     IAGLWESGDGTIKTPEKKETFFVPQKPYILLGSLREQLLYPTFEKDITDQQITETLKT
                     VNLADLPSRIHEAAPEYSDPFDAELHWEEILSLGEQQRLAFARLIIANPNFAILDEAS
                     SALDVDNEKNLYSILNKSTVTYISIGHRPQLSYYHDSVLQLSEDGSWQLLTPDEYRKK
                     LADSLQN"
     gene            8257..9738
                     /gene="glpK"
                     /locus_tag="P5123_00640"
     CDS             8257..9738
                     /gene="glpK"
                     /locus_tag="P5123_00640"
                     /EC_number="2.7.1.30"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016548307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004370 - glycerol kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006071 - glycerol metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol kinase GlpK"
                     /protein_id="HRX14799.1"
                     /translation="MAKYILSIDLGTTGNRVFIFNDKGTPVTSSYAEFKQYFPKPGYV
                     EHDAMEILDSVKNLVKDVLLKTSDVDKKDISCIGITNQRETTLLWDKKNGNPVYNAIV
                     WQCRRTSPYCESLIADGHQDMIRAKTGLVIDAYFSATKIKWLFDNVEQVKDTAKRGDL
                     LFGTIDSWILWNLTGGISHKTDYTNASRTMIYNIHELKWDTELLSLFDIPSTILPEVH
                     PSSSNFGKTKNFGVLPDGIPVTGIAGDQQAALAGQNCVNTGSIKNTYGTGCFILQNTG
                     AMPVASRHGLITTLAADDQGQLAYALEGSVFIGGAVVQWLRDYMHFFDNASQSSKMCE
                     NLNDDNLVFVPAFTGLGAPHWNMDARGAIFGITRDTTREQIVRAALKSIAFQSYDAIV
                     AMKNDSGKEFTTLRVDGGATANDYLMQFQADILRHPVHLPMITESTALGAAYLAGVGA
                     GIYKKVSEIAVNNTITRSFEPVMSSDQAESEITAWNKAVKRIL"
     gene            10015..10212
                     /locus_tag="P5123_00645"
     CDS             10015..10212
                     /locus_tag="P5123_00645"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRX14800.1"
                     /translation="MAKQLLGDILVDNGEISQKQLDEALAIQKKEGGLIGIILVNLGY
                     LNEQSLVKYLAIQAERVVQSE"
     gene            10223..10915
                     /locus_tag="P5123_00650"
     CDS             10223..10915
                     /locus_tag="P5123_00650"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRX14801.1"
                     /translation="MDKITYHQSYQKFRDDNDYFISVFLSDIMDYYKSVKYRYSSIDA
                     AELLIDKLDFYHDIYHHYYTSFVIGAILDFKYALNNYINSDHKFIVRENLDLLIQQIA
                     SRYNRNRRVSLGKINDGKRWVYVTFVRDEVKLISYYDLIVKGCKVKDGYVNFFNRQYK
                     IYYPTPDRSTPTFFIIPDRHGDHCFAADTILQRIQVKGADIIKTYSNVRNSSNKGVFR
                     YKGKRYYTLTPA"
     gene            complement(10924..11541)
                     /locus_tag="P5123_00655"
     CDS             complement(10924..11541)
                     /locus_tag="P5123_00655"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRX14802.1"
                     /translation="MSKGLVVEKTDKHGNIDTIVLSDVKQSLLAGKFVILPVDGIYCT
                     ASILSEKNYFKLSDQGFTDIFVMIKDFSDLERYSRIEKKDYDFLHRLWPDDVYVKLHS
                     TFEESFDTDFFYYIPKTGYLNTLLNQVDQPIMYSFKNNRKSKPYYSITDLKRAYSRVA
                     DRMLIINQYCKEHLSPTFIDIRNKRIQIVSPGRVDDEDIKSLYYL"
     gene            complement(11526..12083)
                     /locus_tag="P5123_00660"
     CDS             complement(11526..12083)
                     /locus_tag="P5123_00660"
                     /inference="COORDINATES: protein motif:HMM:NF016314.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF501 domain-containing protein"
                     /protein_id="HRX14803.1"
                     /translation="MNIWGDTATEEDRRVTGCQINRKNADPYGIIHRCPWNFPVAVSQ
                     KIVDGEGNLHYGNLSNPLWLTCPYLNYEIHELENEGMIRAVNEFILTDREAMERMQDA
                     HAHFYYYRKHLYRHQSGGGISDSLIENFNVGIGGISDTRFIKCLHLHFAHYLVYKQNV
                     AGKIVEELLNKKIYCKDGRCLCLKD"
     gene            complement(12080..12523)
                     /locus_tag="P5123_00665"
     CDS             complement(12080..12523)
                     /locus_tag="P5123_00665"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRX14804.1"
                     /translation="MLNEKNKATLIILVSVLFGIYCFVFGQSGLLERMYLHNQRDILS
                     EEIALLEADNDILFEYLLSNRAGRQFDKEAALQGFIRDGQKRVIINQKDENIEKKSKI
                     TRITLEKSHSETAQLRILWIILSFFIIFLYVVRKQSLATKKGQEK"
     gene            complement(12504..13808)
                     /gene="eno"
                     /locus_tag="P5123_00670"
     CDS             complement(12504..13808)
                     /gene="eno"
                     /locus_tag="P5123_00670"
                     /EC_number="4.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012281796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004634 - phosphopyruvate hydratase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopyruvate hydratase"
                     /protein_id="HRX14805.1"
                     /translation="MFIEDVHGREIIDSRGNPTVEVDVVLSTGELGRAAVPSGASTGV
                     HEAVELRDGDDRYLGKGVEKAVANINTEIYEHITGFSAIDQIEVDRAMMELDGTKNKG
                     RLGANAILGVSLALAKAAADDCSLPLYRYIGGVAACELPVPMANILNGGAHSDAPIDI
                     QEYMIMPVGASSLKEGLRMVAEVFHTLKKILKEMGLSTAVGDEGGYAPNLDNNEHPLQ
                     VIIQAIEKAGFKPGVDKDIAIALDPASSEFYDDKKKKYVFAKSDKRELSSDEMVDYWE
                     DLVNRYPIISIEDGMAEDDWDGWKKLTDRIGDRVQLVGDDLFVTNTERLKKGIEMGVA
                     NSILIKVNQIGTLTETLEAIEMAKKAGYTAVISHRSGETEDTTIADIAVATNAGQIKT
                     GSASRTDRIAKYNQLLRIEEQLGAAGTYKGKEVFYNLAKRKK"
     gene            14331..14921
                     /locus_tag="P5123_00675"
     CDS             14331..14921
                     /locus_tag="P5123_00675"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRX14806.1"
                     /translation="MLKYSKAIEERLESIRSGTPTKNTKKKRRFSRGILLADIILIIA
                     IFFYFNSREDETSLSNGSVVIDGVNYRISSLVNKDDMIITVSLSSREKKQILFENPVA
                     NIVVSYKGVTVLKEYCGIGIESVELESDEARNFFVKVPVSKISDEMKKRGIETEPVKR
                     SFFNKKQGQKFLIRGYFLNSERVELEIVTEVNLKHE"
     gene            14914..15354
                     /locus_tag="P5123_00680"
     CDS             14914..15354
                     /locus_tag="P5123_00680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007289564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytidine/deoxycytidylate deaminase family
                     protein"
                     /protein_id="HRX14807.1"
                     /translation="MSRPDWNTYFMQIAELVATRSTCIRRSVGAVIVKDKRVLTTGYN
                     GAPRGIPHCTEKTCLRTIHKVPSGTMHELCRGVHAEQNAIIQAAFHGIKIEGAAIYVT
                     TQPCSICTKLLINSGITTFYYKNPYNDELALQLMEEAGVERIIL"
     gene            15351..16166
                     /locus_tag="P5123_00685"
     CDS             15351..16166
                     /locus_tag="P5123_00685"
                     /inference="COORDINATES: protein motif:HMM:NF024824.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HRX14808.1"
                     /translation="MKIIAVILLLFCVTINAQETEAVSDPPLEETSTEAKSAGTDSSV
                     SGSESTASPMADHMKEFTGKNFKKGMSYYFQKKFEMAEYHLQEELKENPENYFAYSFL
                     GDIFLIKKRYDEAITLFKRALDLRPDYAENHFRLGQAYYYKNIGNIAISHFTEAYNLN
                     PQIKYSHYHIGLTHLMLLRDKENTIKSWMFYLSIAPEDPQYEKIRRAIELLKDPAFVL
                     PPVGSDIPLEEVLHLGGSVLTKTERSDKDDKGAGHETKKTVDHKADIERDDDL"
     gene            16179..18638
                     /locus_tag="P5123_00690"
     CDS             16179..18638
                     /locus_tag="P5123_00690"
                     /inference="COORDINATES: protein motif:HMM:NF024824.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HRX14809.1"
                     /translation="MIRLPKDLKTQIAIAAGLVMAVGLTAIIAVSVAGDNDLEDIENL
                     QKERIELFEKLLETDPNDVDTMLKLARLYKKIEDYKNAIKYYEKVLEIENDNYEALHE
                     LGYLYSRIGDYDKAIEYLKLAQKYSPDKPKAYNKLGNVYDDQEKELLARDEYTKAKTV
                     APEDQEAYLNLAKQKLKKGDVSGALSEIEDGMKKAPDSAEGFFNLGEVHYKAGNYDKA
                     IEAYNKAIALDPSNAKYYRALGDAYHKNGNRAGAIAAYKKAMDIDPTDFKTAEKIGDI
                     NYEGKDYKDAVASYAHALKYNYDSEILRKKYDDAKSKLASSSIVADKSSGTGDGSGDG
                     SGGGTGSGGETGSGGVSAKSETGYGTSGESDGSGSRDADMDSAITGGTGASAGAAASV
                     AGAASKWRETGDEHRKKREFDDAVKAYRKAVEEDDLDDHSHYWLGRIYYHNKMDEEAK
                     NSFQKAILANPKNADAHYRLGQIYFFEKKYSAAIKAFNQAIANQPEFPKALSARGTAY
                     LRLGDKAEAIKNYKAALAQDANTPGVHFNLGDIYLDSKNFPEALKYFSDHAKLNPSSA
                     EVHFKLGETYHEMGNKTTAVDFYQKAIKFDPNYYQAYFNLGLLYNSRSEFSTAVTNLK
                     KAQSLKPADPAILYELGKAYEGLKDEPAAIDYYTQAIKRNPNYFKAYLNLGLLYKKNG
                     LYDKAIDLYRSAVINNPRSYNAHMNLADTYHGAKMYSNAIDSYNSATELKPGDPVVYF
                     RKGMVYRDSGQYDLAIDSFNRATRLDTKNYKSHYELGMLYYRNVKNNVKAVDHFKKVL
                     AIKPDHPKSSNIHELIDLLSK"
     gene            18649..19092
                     /locus_tag="P5123_00695"
     CDS             18649..19092
                     /locus_tag="P5123_00695"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRX14810.1"
                     /translation="MRIVIFSLLLSVFFTGCVGATSTKSLSNRTESIDSVMSREDKEL
                     LLKYRQEGFADLNSYSVIIVRPIENEDTIEEITEQAQRRSFVSFQKYLRERGVRIDSN
                     TNTKLLNLINEKGSLKRIQDKDKSRVVYIFVVEEDGLKRNIDSIK"
     gene            complement(19160..19711)
                     /locus_tag="P5123_00700"
     CDS             complement(19160..19711)
                     /locus_tag="P5123_00700"
                     /inference="COORDINATES: protein motif:HMM:NF018894.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0035438 - cyclic-di-GMP binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PilZ domain-containing protein"
                     /protein_id="HRX14811.1"
                     /translation="MNKMICFNKLSNIIQNEIIEYHEKKVRSGNTKDLNDSVKQWFDN
                     CFELWLRNKGYVGDNDNKRMFTRIEIEIPLKVSQTLIDDDETDSPEILTKILNISRGG
                     MFFKSPVPFKVSSIIRVEICLSDVEEIWYNIEALAMITRCVKISDNEFGVGVTFSSIY
                     NESMDSLSLFIFKHLVYYIQLEE"
     gene            19997..21724
                     /locus_tag="P5123_00705"
     CDS             19997..21724
                     /locus_tag="P5123_00705"
                     /EC_number="3.5.1.28"
                     /inference="COORDINATES: protein motif:HMM:NF013670.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008745 - N-acetylmuramoyl-L-alanine
                     amidase activity [Evidence IEA];
                     GO_process: GO:0009253 - peptidoglycan catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HRX14812.1"
                     /translation="MYFPKNDIFRSMLFCFLFFFATIAFSQTTKSARIKAILDAEDNA
                     DTRYEIEQIVKTCKYRYSDLYSKLEKGDKITIFIDPAHGKLSNGQWQGGGATFRQSTT
                     EKPEEFYSIPLSRELYKLLVSNKYIRVATTRDYMSVLREESNTYNDISFTETINLANK
                     TNSFLIVSSHLNNISPIHKADGLVNMYGIHITKDRNGSRYISHITSVQRGFLTLYNKF
                     DPTGFSRRIAERTKENHISINMYPNAWDDSIVADDRFSYFSEYPFSVIYESGFISNPV
                     EERFLRIPENQKLIAQQQYLALVASVADIFGVDISNGSLRKAFDQPENLVNDIILARL
                     AVYYIKKNDRKKAIPICNYIAENTNKDIKVSNYYKFLATRLSSVETNIANAYRNRKKT
                     KLFNSYLRKARKAVGGAHIFSSTRKSIIALQRGRKGGHYVASGRRSLPYSKPLTTKTE
                     KHSVTTPFLLTIKPGQTLKEAIDQSIAPSDNYKKKVYSAFSDVKVTTVKWVRNYSKKN
                     KKYIWVKKRYKVKKTFKEGIYVVKLDKKFNVISVKEVKRVTFDPTKYQNQEYFKNSCL
                     SNAVKDRSL"
     gene            21699..22640
                     /locus_tag="P5123_00710"
     CDS             21699..22640
                     /locus_tag="P5123_00710"
                     /inference="COORDINATES: protein motif:HMM:TIGR01212.1"
                     /note="This family includes YhcC from E. coli K-12, an
                     uncharacterized radical SAM protein; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01212 family radical SAM protein"
                     /protein_id="HRX14813.1"
                     /translation="MLSKTDHCNIYNGKPYNYFGDYLRSKYGFRIFKLPINAFLGCPN
                     RINGHGCIFCSESGSASPSIGNHESIYKQMQNAKTSFNRTSVETRYIAYFQAYTNTAG
                     NINTLKKLYDEALSFPDVMGLMIGTRPDEINPEITDLIAEYNVPDFELWVELGMQSAH
                     DRSLDYLNRMHSFSQTRDSIILLSNRGIQVSVHIILGIPGESWADMMQTADTISSLPV
                     AGVKIHHLHVLKNTPLYSMYTNGEVKLLEFKSYISILTDFIERLRKDITIHRIAGDAL
                     EDVLVAPKWGLEKGTVQQRLIDEFNRRCTWQGFLYKD"
     gene            complement(22634..23005)
                     /gene="rnpB"
                     /locus_tag="P5123_00715"
     ncRNA           complement(22634..23005)
                     /ncRNA_class="RNase_P_RNA"
                     /gene="rnpB"
                     /locus_tag="P5123_00715"
                     /product="RNase P RNA component class A"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00010"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00010"
     gene            23165..23872
                     /locus_tag="P5123_00720"
     CDS             23165..23872
                     /locus_tag="P5123_00720"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HRX14814.1"
                     /translation="MLTVDCIKKNFCDNKVLQSVSFHIPQGQVVGLLGENGAGKTTLF
                     RIICSLISADEGNVYINGQALEEDRICYPSVFFGGSSGLYSRLSARENITYFANLNGV
                     YGDVLNNKIDYFSNLLDMNDYIDRKTLQFSHGMKQKTALVRMAVINSPLMLLDEPTTG
                     MDFPSIVSVADFIQHQKNESKTILYSSHNISELSSICQRVIILHKGVIKADIDLIKYS
                     ANKDNIVKKAYLRILEK"
     gene            23869..24999
                     /locus_tag="P5123_00725"
     CDS             23869..24999
                     /locus_tag="P5123_00725"
                     /inference="COORDINATES: protein motif:HMM:NF024091.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HRX14815.1"
                     /translation="MIKSIFIIFKKESIDAIRDVRSLIITFIFPLIVFPAVILLTGSK
                     TDTIYNISVSGIDESIIAHLQADPLLLVSKNEITDKNASLFNYRIDASLSMYNKANFK
                     AIEIEYNNSYKRSIEALEYLKVKLTEYQMNNILENPDIKHTGFIVTFMPIHDIYEGAS
                     MFILYTLLPVLIIIISITSPIAVAADVFAGEKERNSLELLLCNPVSKFAVVLGKYFTV
                     FLFGFMGVLCFLAGFFISVSIDSTFLGISELIITSKQISYSLFLVAILSFVTSSIEVC
                     ISITAKSVKEAQLYILPLTVIFSAINYFSDKSFFSVTDTNISSRLDYIPVLNIMTSMR
                     TVMMNNDINNPLAILLTNIVASIIVLYISKLLLNREKIIYRS"
     gene            complement(25136..25495)
                     /locus_tag="P5123_00730"
     CDS             complement(25136..25495)
                     /locus_tag="P5123_00730"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRX14816.1"
                     /translation="MLKKKSNYLSILKLELEDLKTDIELLIKKCCEDKEEDNISNYVF
                     MENLTLFNNEILGVNVFHAIIDDFASKIDEYSDLEEMIDKLKDTFMDKMKEFGVSKAI
                     YLSILRKMDKVKSYVMQ"
     gene            complement(25500..26627)
                     /locus_tag="P5123_00735"
     CDS             complement(25500..26627)
                     /locus_tag="P5123_00735"
                     /inference="COORDINATES: protein motif:HMM:NF015722.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SUMF1/EgtB/PvdO family nonheme iron enzyme"
                     /protein_id="HRX14817.1"
                     /translation="MKKIAILLFLPVILFSQPEKVFVVKSILDQNRFVFQSEKRLTNR
                     HYNIHLNSVNSGEVTIQSGSRVNSFTNYKYEYIAITSAVVLKKLKTGDTFTFHSKPEK
                     KIPVYNDSFTSEKKSYDKSIVSEVDKREMVLVSGGITLIGSDLGQPHESPVHNKIIKS
                     FYIDKYEVSNRDYLVFCKENAVNAPISWTNGEFPQKMADFPVMVTYYEAEAYAKWAGK
                     RLPTEFEWERAANGVEATQKTVTNLGVIEKPFKTRYPWGNDLHNHVNYNEFWENNTNI
                     PFERGLLPVYYYKERNVSFYGVVNMCGNAAEWTSSWYEKYPGANCSSIRFGQQVKVVR
                     GGDYKSSADEITISSRAYGGLPDLHSDNRSGFRCVKDINRR"
     gene            complement(26629..27288)
                     /locus_tag="P5123_00740"
     CDS             complement(26629..27288)
                     /locus_tag="P5123_00740"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012376.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042578 - phosphoric ester hydrolase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase family protein"
                     /protein_id="HRX14818.1"
                     /translation="MNYLIGDIHGEFSKLLQLIKIIEKDFEPENDELIFLGDYIDRGP
                     DSYKVVEYLYKLSIKYKTIFIMGNHEQMLYDTIYKNESKAIYYQNGGQTTYDSYMANT
                     GSFVIPEHHKKILFSGIHYVERTDFIAVHAGINPNYENPVMCDEFDLMWIRKEFFNDK
                     KKWKKTVIFGHTPTFFITKERNTPYIDENKNIIGIDTGAVYYGYLTAIRMPDRKIYQV
                     G"
     gene            complement(27289..28374)
                     /locus_tag="P5123_00745"
     CDS             complement(27289..28374)
                     /locus_tag="P5123_00745"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRX14819.1"
                     /translation="MEYHDIIKKSIEKLNQDFEIRNSLFLSIIHRNIDALIKNAPSWF
                     GDNPPHEFVDVLNSLVNFSVLIAEQVTNVSELINEKTYQTFSPYSILKVIRYDFLELV
                     VFKRMVISGDKNIEISTSKEVFKDSLYHIFFSISQFITDNTSVETRISEEGSSVLISL
                     IFSDLSEKMPDIKTLLRLFITDDKSDIGTKESHGLKIGLNTAVENIRRIGGMIHIDNL
                     SDYSRLQIIISFPTLSFLQTISDVRKYDIPEPLEKKEGTILVSFADKITELLLSENFT
                     KNGYNVIVGSISKLNLLESYSKVTHIVVDYDSIVDIYESPEDFYAIHSSKRLLVIHKK
                     SVDPVDFDNVTYLSIPLEIDKILEKLD"
     gene            28736..29059
                     /locus_tag="P5123_00750"
     CDS             28736..29059
                     /locus_tag="P5123_00750"
                     /inference="COORDINATES: protein motif:HMM:TIGR00377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043856 - anti-sigma factor antagonist
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="STAS domain-containing protein"
                     /protein_id="HRX14820.1"
                     /translation="MDISIREHGDNKIISVSGEVDLYNVSELKNALFSLTDGSNSSVV
                     VDMKEVNYMDSSGIGALVAGQKKMKAHNGSFALMNISDDVLNILKLATLDKFFKIYYN
                     ESELV"
     gene            complement(29062..30624)
                     /locus_tag="P5123_00755"
     CDS             complement(29062..30624)
                     /locus_tag="P5123_00755"
                     /inference="COORDINATES: protein motif:HMM:NF018884.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016791 - phosphatase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SpoIIE family protein phosphatase"
                     /protein_id="HRX14821.1"
                     /translation="MKNKKNIADAKKELKNSKKKRVIFSLKFKFSIAIIVLTVTIISS
                     MAYYFLQKETLLLTDNIKDNARLMMNTFSAVTQTSLGDNDLVIYDTINIFKQNKSFRY
                     AYVTDPEGSVIISDNPDKIPDNSKDEHLKKAINNSTIKNVLEQEIKDNQDENGKIYDF
                     IMPVYSRLNDSRIAIIRLGFSDALIRDQIKKLQNTIIVIALIFIGASVIASFILSYIT
                     TNPLKHLSNGVAIIATGNMDHKITIKSKDEIGRLASEFNSMTLELKEAKNKEIESRLL
                     AEQLELAKEIQEGLNPMGFYNSRGVQIQGYTRAAKGVGGDYFDYIDIDDSRVGVLISD
                     VSGKGVPASLVMVMIRTVFVSSVDQNSTSVQCKNIVTAVNNSLSADFAIDKFATMMFI
                     IFDRASGTLSFTNAGHGPLFCYRAKEKHCTVTTLEGMPIGIVDEASYLQSSTRFDVGD
                     IVVMYTDGITEMRNSNREEYGRTRMQKILMQNCEKSAEEIVKTIIEDVEEFRGETPPH
                     DDMTLVVVKRTE"
     gene            complement(30632..32557)
                     /locus_tag="P5123_00760"
     CDS             complement(30632..32557)
                     /locus_tag="P5123_00760"
                     /inference="COORDINATES: protein motif:HMM:NF024824.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HRX14822.1"
                     /translation="MKKQSLHYLFFVLILFFFNLYGQTVSELDNRAQRYYEAGEYERA
                     IVDWITALEKSPGNKQIQHKVEMVYNRKRTRDLAFERSKKFLRIARTHLEKKEAQKSL
                     DNADKSIENFITAYRITPRDPEMQILRARVERMRADALQELERQRLHAQMKKKYDDHY
                     AAALEFMENKEYENAITEWDAMLVIFADDNIAKEGKRKATLALQSRMRFEQIKRLTEN
                     GIRLFEETKYQESLHDFEEILKLDPGNPDAENYIAKIDDILEEEQKLERKRLQAERYY
                     SSGLQNSNEYKFDQAREDYEMVISLIEDYKDAKSRLAGLDKLEEEYNERIRQQNLEKI
                     NRQFQAGLLYLAQGDYESAVTAFENIIEIDPANDQARRYVKTSKEALAQEKEDIIDES
                     SPYYSLVSTLISSGKLYYERQEYRKSLDQWERILNLFPNNTFATEYLLKCQLKLNPDI
                     YKKFAAEQIEEGKKMLKQKNYPRALRRFSIIKSISPDYPDIDNLIASAKPSKPQIPIP
                     AGTPPPRITVSPAEIEQRYNRGIKLYRNGDFKKALSEFKWVVNNDPQNTRAIINVNKI
                     ESEIRLAGNPAKKRSATLSEEQKKLVRQYYFAGINYYSQNKFAEAVSEWRKVLAIDPN
                     HDKARNNIRNCLLLMKQ"
     gene            complement(32621..33064)
                     /locus_tag="P5123_00765"
     CDS             complement(32621..33064)
                     /locus_tag="P5123_00765"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRX14823.1"
                     /translation="MKLNRIVQFLIIALLLTNCTKQKSNNPDTDDMEKIKKLFTVFEV
                     ALKKNDYSLYRNVFTESSTKDDFEKKFGDNYYKNIQIMNIVFKGEQIIGDEVAFVNNV
                     TIAADKYARDNDEYIGMIRADAVVFTPKEKSVQWKILHMITVHNE"
     gene            complement(33051..33617)
                     /gene="amrA"
                     /locus_tag="P5123_00770"
     CDS             complement(33051..33617)
                     /gene="amrA"
                     /locus_tag="P5123_00770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006927544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AmmeMemoRadiSam system protein A"
                     /protein_id="HRX14824.1"
                     /translation="MDISKSVKQSLLKIARLTIASQLGIKMPYSAESIDTSDLIFNKK
                     CGAFVTLHLNGSLRGCIGYIVGIKPLLQAVKDMAYSAAFKDPRFLPLTSAEFEQIDLE
                     ISVLSEIEEVTDISRIVVGRDGIIITKDHSSGLLLPQVATENNWDLETFLGHTCLKAG
                     LDAESWKDESTVIEKFSAEVFGENNEIE"
     gene            complement(33630..34442)
                     /gene="amrB"
                     /locus_tag="P5123_00775"
     CDS             complement(33630..34442)
                     /gene="amrB"
                     /locus_tag="P5123_00775"
                     /inference="COORDINATES: protein motif:HMM:TIGR04336.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AmmeMemoRadiSam system protein B"
                     /protein_id="HRX14825.1"
                     /translation="MYIRQTAYNGTFYPSDKAKLAAEIDDYLENASSVIKHPTAKGII
                     VPHAGYVYSGQIAAYSYMSSSDCKPSKIIVLAPSHRYSFEKGSVIPDGLYRTPLGDVK
                     IDVSAADLSQKSSMTFLKAAHDSEHSIEVQVPFLQTVFPNADLIPVLIGSQDPSICTS
                     IASDIASIYEDEKQKPLVVISTDLSHFHRYDEAVQIDNTTMEIITEFNEDTLYHSVQN
                     GSEACGFAAIYTGFKLLKKFGAKTCEKLMYKNSGDASGEKNRVVGYMAAVIY"
     gene            complement(34444..35112)
                     /locus_tag="P5123_00780"
     CDS             complement(34444..35112)
                     /locus_tag="P5123_00780"
                     /inference="COORDINATES: protein motif:HMM:NF016051.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopantothenoylcysteine decarboxylase"
                     /protein_id="HRX14826.1"
                     /translation="MASDKDFSYLVSAGPTREWIDPFRFISNPSSGKMGIAIADEVYK
                     HSNRIKLLHGVIDNNLLKNKAYECKGCETTNDLLRAMEKELSSDMIIFMAAAPSDFRP
                     VNYSDQKIKKNCDRININFEKNPDILMHIAQLKLSGNYKNIITVGFAAETHNTENYAL
                     SKLKQKSLDFICLNDISKKGVGFAGETNEVTIYCKSGEKILIPMDTKVNVAKQIFHNV
                     TRRI"
     gene            complement(35105..35644)
                     /locus_tag="P5123_00785"
     CDS             complement(35105..35644)
                     /locus_tag="P5123_00785"
                     /inference="COORDINATES: protein motif:HMM:NF014494.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavoprotein"
                     /protein_id="HRX14827.1"
                     /translation="MKIVLGVTSSIAAYKACDITRLFVKEGHDVFVVMSKNALNLVSP
                     LTFRTLSGNPVYHETFLPDVYNMGHIALKENANIFVCAPATANFIGKAASGIADDLIT
                     TTFLSMKTPIVIAPAMNPDMWSNPAVQHNISVLKKRNITIIEPDTGDVVCGDTGYGKL
                     APVNIIVKRSLELLENQNG"
     gene            complement(35641..37554)
                     /gene="mutL"
                     /locus_tag="P5123_00790"
     CDS             complement(35641..37554)
                     /gene="mutL"
                     /locus_tag="P5123_00790"
                     /inference="COORDINATES: protein motif:HMM:TIGR00585.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006298 - mismatch repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair endonuclease MutL"
                     /protein_id="HRX14828.1"
                     /translation="MKTDSKTIPSIHELPEHVKRMIAAGEVIEGPHSVIKELIENSAD
                     SGAGRISVKIEDGGMRRIVVTDDGCGILPEELKLAITEHATSKITSVDDIQSIYSYGF
                     RGEALSSIAAVSHITILSRPHNLDEGAKLVSDDNISVLPFAGPVGTTVIVEHIFYNTP
                     ARKKFLKARSTEIRLIRESFIKIAIANPAIVMTLENEDKVLMSLPAREHIRERLADIY
                     GSNEASRLLEGSVQDLKVNINGFISSLEGLKKSRSMQFLFVNGRPVEYKNLSFHVNRA
                     YDSMLPKGLHPVLFLFITIPPDLIDVNVHPCKREIKLFDTRYIDSLIYGFVKKTLGQN
                     EIHLDSSVFTNFKNDNISERELHSSSYENEADHDQSNLWQIADNDLNSKNVSQKNNEG
                     SRISKNDEINGGFNNLGNCEIMNDYTDDPLKTGPSKTVSYFNDGRDIYNQLHNWYYIG
                     TLFKTYLLVEKEESLKIVDFHAAHERIIYDRLIKNEEKPSVQKLIFPEEVNMDIQEVS
                     FVSDNLDVLLSMGFDLDIFSQESLVIRGIPETLDPSQAVTCIREFTQSISDLSLDRNA
                     EDYFIQIKKHAAERVACHSAKRAGDPMSRIDAESLLVKIFSGSYPLRCPHGRPFVADI
                     SRKDFEKLFRRLI"
     gene            complement(37541..38032)
                     /locus_tag="P5123_00795"
     CDS             complement(37541..38032)
                     /locus_tag="P5123_00795"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRX14829.1"
                     /translation="MKKSLFLIILALMLASCGLTDSIIVKQVNPDSMIHYSNIYSFDG
                     ELADEDYYVLYFTAGDRIPLRIVLNNDYVRVDENSDINMIAKRNIYFMIKNPENSSNR
                     SNYKISRNSRIFISFDAVRWSSIHNSETLKEIAGFDEESVSFGFEATNQDGIRSQLTI
                     ENR"
     gene            38482..39390
                     /locus_tag="P5123_00800"
     CDS             38482..39390
                     /locus_tag="P5123_00800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013313269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor RpoD/SigA"
                     /protein_id="HRX14830.1"
                     /translation="MSSKNDTEISPVVDKSTVKLKRNRKKGFDSSLAWYLDKINKIKL
                     LTREEEDYYARKALSGDPEAKEMLIKSNLRFVVSIAKKYQTSGISFLDLINEGNMGLI
                     KAAEKFDPDKGFHFISYAVWWIRQAIILAISQKTSLIRIPLNRTADLQKIEKVHKRLE
                     HEFGREPTPQEISEFLDMDNEQINHLRNISQDYISLDTPYGDSDDTTMISTVEDIHTD
                     APYKDLIDESLREELSSVLDTLTVSEKDILVKRYGLDGNKPMSLHQIGEQYSLSKERI
                     RQIEKKAIRKLRHPSRSFKLKSYITD"
     gene            39477..40559
                     /locus_tag="P5123_00805"
     CDS             39477..40559
                     /locus_tag="P5123_00805"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRX14831.1"
                     /translation="MKFLFLGIVLGGLLAFIPYSEIDNGARVTPEWFGSVEKIRSIDP
                     SNNINKTKGYFLYPSLDHAYLLNADGSFHKEYSGDENSRFAFSGDKEYYIQFEKVGSH
                     ISFLNRTGAKFWITETPQYPYLSHSAKLVLLLVSDLHTIFLNDKNGKPVGIQKIEGRM
                     CTNISFSKNDYAIAGFMSSEYYIVDSSGNLVLHGKTPEGTIVKSCVLSDNSSYAAIHY
                     GTVNEDGCMIINISNIPDGDFELSVKMESVHKTRTGIAISDTGTLHLLDGLYYRMFDN
                     KGNPLFAKEIEPQSHGHAQISLYNGLAIASYRLFGGKSILSIYDNDLNILHSETFEDT
                     ALNIEITGDVLFAAGTKNLSAYSLAR"
     gene            complement(40495..41373)
                     /locus_tag="P5123_00810"
     CDS             complement(40495..41373)
                     /locus_tag="P5123_00810"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF021616.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family A protein"
                     /protein_id="HRX14832.1"
                     /translation="MTSLFTVIIPVFNREKTIRRAVDSVINQTFSDFKFVIVDDGSTD
                     KTSSILSKYKDSRITVIRTENSGVSSARNTAVKDLITPYTAFLDSDDEWLTNKLENDY
                     QFIQENPQYHVFQSQEIWYRYGKRVNPKLKHKKKSGSIFNESLEQCMISPSSVVIHKS
                     VFENHGLFDPNMPVCEDYDLWLRITPFYEIGLIDKQTIIKYGGADDQLSRSVTAMDRF
                     RIYSMLKLLNNTKYGEYQKKIEPVLKRKINIVMAGAIKNSNKNLFKNLNETLNIIEQG
                     CTPRDFSYLLQKEHHL"
BASE COUNT        13109 a         7623 c         7206 g        13535 t
ORIGIN      
        1 aattacagaa ggtgtacaac ataataaatc accgttctga attatctcgt atgtactatt
       61 tgattcgtta tagtttattg taatattttt accgcttttc tttttaccaa tggagatact
      121 gttattttta aacggaagga atgtcaccag gtcatcaagt gaaaaactgc ttctgaagta
      181 atcgatctct tttttaagat caaaataacg attcttactc cccggataat cggtttcaaa
      241 atcgacatgt acatcaagat tttcgggacc gaaaagtgac tcttgtagta cctctctcat
      301 tcggactatc tgctctttac ggcatattac atgagttttc tgttttctaa caaaaaaatt
      361 aaaatagaga ggaaattcaa tttcagcaac acttatccct tttatccagt caaaaagagt
      421 tgcaggtaat acaaaaaaag agggtacgcc tctttccata atcattgtgt cttttatggt
      481 ctcgggtgga acacctatct ggacggggcc aagacatgta tctacaagat aaccaccccg
      541 ttttagttgt atataatcag ccatatgcct catcaattgc tcgcaaaatc tattgaatgc
      601 agtttaagtt tgcttaaatt gcattcaata gatttgcaga ttattaaagg actctatttt
      661 aaccagagag catcagagag tcaaataatt tattaatttt agcacaattg ggcttttggt
      721 tggaaggtca ttttacaatt tctttttaac aatatcatat atctctttgc catctttgct
      781 ctgataagga aacgaaaaaa taatcaggta gttatcctta attaacatca actcagggta
      841 ctcttttgaa cctggcttcc cctgcccaaa aaaaagtttg gtcaaaaatg atttatgacc
      901 atcgtccagt ttctcgtcaa aatagtagta aaaaacagtg ccctgctctc cgttgttatc
      961 cgtgctctgt gtgtattttt ctgaaataac gggtaaaata tgtctgtagt tattcgagtt
     1021 attataaaaa agagcctgat gattgctata aaagttttta atatgtgaaa gattatactt
     1081 ttttatttca gaagggttta attttatttt ttcaaaattc ttggtctccc ctatttctaa
     1141 tcgatcatta acaagatcgt aaatatcaga tagaaaagca gttttaactt tcttgtcatt
     1201 tgacaaaatt gtaaaggagc ccaatagata attataacga attatcccct gtatcagata
     1261 agcatattct ccttctttgt tagacacatc gtttttatca gttaaaaaat agaaatatcc
     1321 tttgatttta ccattcttat tcaactcttc gatagatagc tccttttcaa cagcacttgg
     1381 taaaagttct tgcccctgtt tagtaataat actttttagt tgttcattac ttgttttgtc
     1441 agagttgtag ggataaagcg tggttgtaat ttgataatca ccttttttac ttggataaag
     1501 aaacatatcg tagccatatt cgtcttcacc aattttaaaa ttatctccac tccactcttt
     1561 tataacaatc ctgacattat cacatacaaa aaaaatattt ccttcatcag aactgagagt
     1621 tgtaacacaa aaaaatacaa acataacaat tagtagctta attttcataa tatttcctgg
     1681 attttaaaat gtataataag tgtgaggctc ggtaaatccg tttaatccat aatctgctga
     1741 cttttgaata aaatccttgt agttatcacc atagtgaaca agattaattt ttcgtttaat
     1801 attatctgaa aaaccattaa gctcatcaat tgatgcgtga actccacctg taaaaagctg
     1861 acagtcatga aaaattgctt ctaactcgaa acgtgaatca aaatactcaa ctagctctct
     1921 gtcaaaacgt gtatcactcg tatacattac tttatcatcg atgataactc cacaactcca
     1981 gaacgagctt tcccaatccc ttgaattatc aggtatgtgt ttggtacgga aaattttcag
     2041 attaatagag ccaacgttag cttcaagtgt ttcacgctga tatccctgta aaacctttgg
     2101 cctgtttatc ccgaaaaaat ctgaaaaagt aagaatgtca ccggcgtttt cttcgttata
     2161 tgcagctccg cctcttagcg acatttccca taaaatatat tgataggtct cgtttattgt
     2221 cataacaggt ttttttttgg taacatattt attcatcaag gcaacttcct ctaagccacc
     2281 tatatgatca gcatgcgaat gtgtaataag aaagttctga acttctgtaa tggaactacc
     2341 aagatcatta aatgcttgtg ggcattttgt accgcaatcg acaagaagat gatcatcccc
     2401 tttaattata agaagatttg tctggtaatt gaattttgta aaagcactac cggtaccaac
     2461 aaaaaacaac tgaagagctc catcgttttt taacgaaaag ggctgatcat ttaaagtaat
     2521 ttttttcata aaaccgcctt atggtgcttt aatttacttg atgatctatg gaaataataa
     2581 ccatacaatt ttgatctgct ttgaacaata atgtcaataa aaaaaaggat caccaattga
     2641 cagaataact atatttactg ttgttttaat tgacttaagt ttaagtgttt acttaaagtt
     2701 gttcgtaaat tgattacagg gaacttcgaa aaattaccat ttctggagcg gctatgccga
     2761 ttgaagccaa ttaaagacaa tttttctcgt tgtcactcga aataacgctc ataaatatcg
     2821 cttgttattg attatttttt tcgcaagctc aaaaaatcgc tgaaatttta agctttaaag
     2881 acagcttcaa atttcagctt agtaagcgat atttatggct ttattaaagt aattttttgt
     2941 attacccgtg ggtcttttat cttgtgctaa aagcttattg ttctgataag aacaacctac
     3001 aagggcaagc tcctctgctg gtcgtccagc accattttct ttaaaaatgg tgctggacga
     3061 ccagcagatg caacctggtt aaaaagttaa ttattagaga ttgcctgcat ttaatgccaa
     3121 atacaaaaat ccagagttag aaagggcaag ttgtagtaaa ggttacaaat tccagtttca
     3181 cagaggtttt atgatgatta ataacgagtt ttgggaaagt tgtgttgtta caaaaagact
     3241 attatcgaac gaaaaagctt ttgataacga atctcgttca ccttttgaca tggatcacga
     3301 tagaataatc tattccaatt tttttagacg catgcatgat aaaactcagg ttttcccata
     3361 cgctccgctc tcaaattctg gtcaggcgcg gtcaaggctt tcccattcac ttgaagtttc
     3421 atgcgtggca agatcgctcg ggaacctttt ggggcactac cttgttaaaa atggtgcaaa
     3481 aataagacct tacgatattg gcatgattgt tgcgtcggcc tgtctagcac atgatatagg
     3541 caatcctcca tttggtcact ccggtgaata cgctattcaa agctggatat cgaataatct
     3601 tgaatttgcg cttaaagata aatgggaaaa aaacgatata atgaattttg aaggtaacgc
     3661 gcagggtttc agaatcctct ctcgacttga aagctgtcag cggccaggcg gcttacgacc
     3721 gacggttagt acgcttgccg cctttatgaa atacccctgc gcatctatag atacagacaa
     3781 aaacagtaat aacccaggat taaaaaaatt tggttttttc aaggccgacc gtgaaagttt
     3841 cgatgatgta tttgattcat ttaatattcc gcgtcaaaaa aattgcgtta acgcgtatac
     3901 tcgacatcca tttgcatttt taacagaggc tgcagatgat atctgttacg caatagtcga
     3961 tcttgaagat gcttatcatt tgagtgttat cggttttgat agtgtttatg atcttttaat
     4021 gccgattgtc agttcaaatt caagttttaa ggacaacaaa gagttcgaca aggatatcag
     4081 agtcgccaga atgcgttcag ctgcgataag tgtattgata gatcagacca tagatgtttt
     4141 tacagataat attgaacttt ttgaaaaata ttctttttct actccattga taaataaaat
     4201 tgatctttca tccagctaca gtgtaataaa gaatttttct tttaacaaaa tttataattc
     4261 aagccgtgta cttgaaatag agtgtgctgg attcaaaaca attggaggcc tttttgatat
     4321 gtttgttaat gcagttttga ctgataagcc tgtggcacaa gaaaaaatca gcagacagct
     4381 tataccttca caatatttca aacggcatga aaatagtatt cagtcgaaca atcataatca
     4441 gcttatagaa agactttcga cttacgaaag aattttatcc attaccgatt atatctgtgg
     4501 aatgaccgat cgttatgctg tcgagttgta ccagcgaata tcaggtatta gattgccggt
     4561 ctattagaaa agcattttaa aactgaatat tgttacgtcc actttctttg gctttgtaaa
     4621 gaagattatc ggcaattgaa acaagctcct ttgatgtttc cttaatacat tcggccacac
     4681 cgccgcttat agttacatta agatccgtgt tttcaaatgc aagtttctct atagaatcac
     4741 gaattctttc ggcggtttct acagcctgtt ttaacgatgc ctttggtaaa ataatcataa
     4801 attcttcccc gccgtaacgt cccgccaaat caacatttct gatatttttt tgtattattg
     4861 atgataccga tttaagcacc ttatctccga aaagatgacc ataggtatca ttaatatttt
     4921 taaaaaaatc aagatcaagc ataatcagag aaagtttatc tccgtaacgg ccttttttct
     4981 caatttcaag atcaagttgc ctgaagattt ccatgtggtt cagaagtcct gttaatccgt
     5041 ctgatttggc cattttttca agttccaggg ttttctcttt aacttctttg tataatgaat
     5101 aggaccttgc gcaagtgcgt attcgtgcca taaaaatatc gttgttgtat ggctttgtaa
     5161 tataatcatc agctccaagc gataagacat gagaaatggt ttttacgtta tcaatgcctg
     5221 aaatggcaat tataacactt ttgcatccat cggatcttaa tttttttata acctgatgtc
     5281 ccgatgaatc tttgagtata agatctacaa gatagatatc gtaggttctt ccggcttcga
     5341 acatctcttc agggtcttca taaaaatcta cacgattaat tgaatttatt ctgaaaattc
     5401 tttcagtaat tttccggtca acttttgaat catcacaaac agcaatatac atatcagtaa
     5461 gatactgggt ttttgattta accgcaaagg cctttaagta gtcacgaatc tcgtctcgag
     5521 atgatttttt cacaatataa tcgacaatac caagatcgta cattttttta cggtcttcaa
     5581 atgtatcgtt accggttatt acaacaatag gtacttttga ataggatctg tttataagtt
     5641 ctataaaacg ttcaatactg ccctcgcgaa tttccattgc ggtaactatt aatgaaatat
     5701 tgtcggttga caatactgca agcgcatctt ttgatgaaga gcagctgaca taatcgatat
     5761 caagctcatg gcagatatcc gatatcagct tttgataaag agtactggtt tcaatatgca
     5821 gtattttaaa cataaagatc ctttgccaaa ttatatattt atgtgtctgt taagcccgtt
     5881 gatatcttaa aaaaaacatg atgaaaataa aaaatgcaaa ttaataatat ggataatttc
     5941 tagtgtataa caatagctaa tttctattta tagagcaact attttttact tgccataaat
     6001 tttcttgtga cataacaggt attagcaata ataaataact gtggcaaaaa aaaattctgt
     6061 tttaaataat ttctattata atcgactaat tgattcgttt tatatcggga attaaaggaa
     6121 ttacttttta tgctgccaaa tatacggtaa acaatgaaaa aaaacagaca aaaaaaaatg
     6181 cacaatgtta ttcactatct taaattaaca catcaatact ggtttccgtc aggcaagtta
     6241 agcattccct ctttcaccct tcttcttact gccttgatat gttttatcgc ttctatgcta
     6301 tatctttttc tgaatcttat tgtagcatcc ggaaaattta ttccccgttt aactagtttt
     6361 ctatcttcag ttttttcggt gccattatta accgtacccg aagatttctt gtccagaatc
     6421 gctccagggc ttacagagtg gctcgcaggc attagaggag gaaatctaaa tacctttttt
     6481 attgcaagct cagtcgtagc tattgtgatt tttctgctct tccgtaaaaa gataaaaggt
     6541 cgcaaactgc cctggattat tcttttcgta ttgcttacac tatccatgat ggtaagtggt
     6601 gttaacgtaa ttttaagtta taccggtcgg ttttggcaaa cagcactgca gcagcgggat
     6661 accgtaagct tctggcgttt tatttttgtt catttgggaa tgtatgttgt tgcgatgccg
     6721 atagttgtgt tttacccctg gatacagggc atacttggaa taaaatggcg taaatggctt
     6781 accgacaagt ttgttggatc gtatatgaaa aacagagcct attacgagat aagcaataat
     6841 aaagagattg ataatcccga tcagcgtatt tcggaagacc ttaaccgttt taccgtcgaa
     6901 agtcttgact atttgcttat aatactaggc gccattattg accttacagc atttaccctt
     6961 atactctggt caatttccca aaaattgaca ggcattttaa ttgcatactc gttttttgga
     7021 acagcttgca ccattttcat tggaaaaaaa cttatcggtc ttaactttga acagcttcgc
     7081 cgtgaggcaa actttcgtta cggccttgtg catattcgcg acaacaccga gtccatcgcc
     7141 ttttacagcg gtgaatcaac cgagaaaaaa aatctggctg tacgctttaa aagtgttgtt
     7201 tcgaactata ttcggcttat tggttgggaa agaaacctaa agttttttac caaatattat
     7261 cactacatgc aggtacttat tccctatata atcatggctc cggcctactt tcgagaggag
     7321 atcgaattcg gagtaatgta tcaggccagt tttgcctttg caatgataat cggtgccttc
     7381 tccataatta tcacatactt caattcaata accagatatg ttgccggaat tcagcgtcta
     7441 acatcctttg aagatgtact catcaattca cgtggtattc gtaaaattga gggtcaaaag
     7501 ggatcttaca aaataaaacg tgaaaaagca ccgctaattg aggtttccaa tcttacactt
     7561 aagactcctg atttttccaa gagtcttgca cgtaatctct ctttccgtat agataaggga
     7621 gagagtcttc ttatagttgg tccttctggg gttggcaaaa gttctttgat acgaactatt
     7681 gcaggcttgt gggaatcagg cgatggtact atcaagactc cagaaaagaa agagacattc
     7741 tttgtaccac agaaaccgta tattcttctc ggttctcttc gcgagcaact gctctaccca
     7801 acttttgaaa aagatataac ggatcaacag atcacagaaa cgctaaaaac cgttaatctc
     7861 gctgatcttc cgtccagaat acacgaagcg gctcctgaat acagtgatcc tttcgatgcc
     7921 gaactgcatt gggaagagat actttctctt ggagagcagc agcgactcgc tttcgcaaga
     7981 ctgattattg ctaacccaaa ctttgcaatt cttgatgagg cttcgagcgc ccttgatgtt
     8041 gacaacgaaa agaatctata ttcaatatta aacaagtcca ctgttacata tatctctata
     8101 ggtcacagac ctcagctttc ttattatcat gactccgtat tgcaattgag cgaggacggc
     8161 agttggcagt tattaacacc ggatgaatat aggaaaaaac tggcggattc cctgcaaaac
     8221 tgagacaggg ataataattc ataacaaggt ggtcttatgg caaaatatat cttatccatt
     8281 gatcttggaa caacaggcaa cagggtattt atttttaacg ataaaggtac acctgttaca
     8341 agctcttatg ccgaatttaa acagtatttc ccgaaacccg gctatgtaga gcatgacgct
     8401 atggagattc ttgactctgt taaaaatctt gttaaggatg ttctgttaaa gacgagtgac
     8461 gtagataaaa aagatattag ctgtatcgga ataacaaatc agcgagagac aacgctgtta
     8521 tgggataaaa aaaacgggaa tcctgtttat aatgcgattg tatggcaatg ccgtcgtaca
     8581 tcgccctatt gtgaaagtct aatagctgat ggccatcagg acatgataag agcaaagacc
     8641 ggacttgtta ttgatgccta tttttcagct acaaagatta agtggctatt tgataatgtt
     8701 gaacaggtta aagatacagc aaaaagaggc gatctgcttt tcggtaccat tgacagctgg
     8761 atactgtgga acctgactgg aggcatttct cataagaccg attacacaaa tgccagccgt
     8821 accatgatct ataatataca tgagttaaaa tgggatactg aacttctatc gcttttcgac
     8881 ataccatcaa caatactacc tgaagttcat ccatcttcgt ctaattttgg caaaaccaaa
     8941 aattttggtg tgttacctga cggtataccc gtaacaggta ttgcaggtga ccaacaggca
     9001 gcccttgccg gacaaaactg tgttaataca ggtagtatta aaaatactta cggaaccggc
     9061 tgctttatat tgcaaaacac aggtgccatg ccggtcgctt ctcgacatgg attaatcaca
     9121 accctggcag ctgatgacca agggcaactt gcatacgcgc ttgagggctc tgtgtttatt
     9181 ggtggggctg ttgtccagtg gttacgtgat tatatgcatt tttttgataa tgcatcgcaa
     9241 agctcgaaaa tgtgcgaaaa tcttaatgat gataatctgg tttttgttcc ggcttttaca
     9301 ggtcttgggg ctcctcactg gaatatggac gcgcgagggg cgatattcgg cattactaga
     9361 gacacaacac gcgaacaaat tgtgcgcgcg gctttaaaat cgattgcatt tcagtcttat
     9421 gatgcaatag ttgccatgaa aaatgactcg ggaaaggagt tcaccacctt acgtgtagac
     9481 ggcggagcta ccgcaaatga ttacttaatg cagtttcagg cagatatatt gcgacatccg
     9541 gtacatttac caatgataac cgaatcaaca gccctaggag ctgcatatct agccggagta
     9601 ggcgctggta tttataaaaa agtcagtgaa atagcggtta ataatactat aacacgttct
     9661 ttcgaacctg ttatgagttc cgaccaggct gaatcagaga taactgcctg gaacaaggcg
     9721 gttaaacgta ttctgtaatc gattggtcag ttatacgcgg cataaaaagt atttttgcga
     9781 cttaattatt gatctttttt cgtttattta ttacttttgc aatataacta ttttgtaatc
     9841 aggcagttta tactctgctt ttttattacc aaaatagctt atgtaactat ctaacagtgg
     9901 atttttcatg gtctctctgt tggccgttaa taaccgcttt gttaaagcaa agtgaaaaca
     9961 aaatattaaa agcatattat ctgatatgct ttactggttt tttctggagg agatatggca
    10021 aaacaacttt taggcgacat tcttgtcgat aatggtgaaa tttctcaaaa gcagcttgat
    10081 gaagcgcttg cgattcaaaa aaaagaaggt ggacttatcg gaataattct agtaaacctt
    10141 ggatacctta acgagcagtc tctggttaaa tatctcgcta tacaggccga aagggttgtt
    10201 cagtcagaat aattcaatcc taatggacaa gataacctat catcaatcct atcaaaaatt
    10261 cagagatgat aatgattact tcatctctgt ttttttatcg gatataatgg attattacaa
    10321 atctgtaaaa taccgctact cctctattga cgcggcagag ttattaattg ataaactcga
    10381 tttttatcat gacatttacc atcactatta tacatcgttt gttattggag ccatacttga
    10441 tttcaaatat gcattaaata attatattaa tagtgatcat aaatttattg tcagggaaaa
    10501 tctcgatttg cttattcaac agatcgcttc acgatataac cgaaacagaa gggtttctct
    10561 tggaaagata aacgatggta aaaggtgggt ttacgtcacc tttgtccgtg atgaggttaa
    10621 gctaatatca tactatgatc ttatagtaaa aggttgtaaa gttaaagatg gatatgtaaa
    10681 cttctttaat agacagtata aaatatacta ccctactcca gacaggagca cacccacatt
    10741 ctttatcatt cctgataggc atggtgacca ctgtttcgcg gccgatacta ttcttcaacg
    10801 catacaggtg aaaggagcgg atataataaa gacctactct aatgttcgaa actccagtaa
    10861 caagggtgta ttccgttaca aaggtaagag gtactacacc ctaaccccgg cctgattgac
    10921 taatcaaaga taatataatg attttatatc ctcgtcatca acccgtcccg gtgaaactat
    10981 ctgtattctt ttatttctaa tatctatgaa tgttggcgac agatgttctt tacagtactg
    11041 attaattata agcatacggt cggcaactct tgaataggca cgttttagat cagttattga
    11101 gtagtaaggt ttgcttttcc tgttgttttt aaagctgtac ataatgggtt ggtcaacctg
    11161 atttagcaaa gtgttcaagt aacctgtttt tgggatataa taaaaaaaat cggtatcaaa
    11221 tgattcttcg aaggtgctgt gcaattttac gtaaacatcg tctggccaaa gtcgatgtaa
    11281 aaaatcataa tctttttttt caattcgtga ataacgttca agatctgaaa aatcctttat
    11341 cattacaaaa atatccgtaa aaccctggtc gcttaactta aaataattct tttctgataa
    11401 aatggaggcg gtgcaataaa ttccatcaac tggtaaaatt acaaattttc ccgccagaag
    11461 agactgcttt acatcagaga gaacaattgt atcaatgttc ccgtgcttat ccgttttctc
    11521 tacaactaat cctttagaca taaacacctt ccatccttac aatatatttt tttgtttaaa
    11581 agttcttcca caatctttcc ggctacattt tgtttgtaga caagatagtg ggcaaaatgg
    11641 agatgcagac atttaataaa acgtgtatca gaaataccgc caataccgac gttaaaattc
    11701 tctataagag aatcagatat tcccccgccg ctttgatgcc tgtacagatg tttgcgatag
    11761 taataaaaat gagcatgcgc atcctgcatc ctctccattg cttctctgtc ggtaaggata
    11821 aattcgttaa cagctctgat cataccctca ttttccagtt cgtgtatctc ataattaaga
    11881 taagggcaag tcaaccaaag cgggttggaa agattaccgt agtgtagatt accttcaccg
    11941 tcgacaatct tttgacttac agcaaccggg aaattccacg gacatctgtg aataattccg
    12001 tatggatccg cgtttttgcg gttaatttga caaccagtaa cacgccggtc ttcttccgtc
    12061 gcggtgtctc cccagatatt cacttttcct gacccttttt tgtagctaac gactgtttcc
    12121 taacgacata caagaatata ataaagaatg agagaataat ccacaaaatt cttagctgcg
    12181 cggtctcgga atggcttttt tccagtgtta tgcgggttat ttttgatttt ttttcgatgt
    12241 tttcgtcttt ttgattaatt attacccgtt tctgtccgtc acggataaat ccttgaagtg
    12301 cggcttcctt gtcaaactgt cgtcctgcac gatttgacag tagatactca aacaggatat
    12361 cgttgtctgc ttcaagcaga gctatctctt cagaaagaat atctctctgg ttatgaagat
    12421 acattctttc gagaagaccg ctttgtccga agacaaagca gtaaatcccg aatagaacag
    12481 aaacaagtat tattagagtg gccttatttt tttcgtttag caaggttgta aaagacctct
    12541 ttgcctttat atgtacctgc tgcacctaac tgctcttcga ttctaagcaa ttggttatat
    12601 tttgcgatac gatcggttct tgaagccgaa cctgtcttaa tctgacccgc atttgttgca
    12661 accgcgatat cagctattgt tgtatcttcg gtctcgcctg aacggtgaga gataactgca
    12721 gtataacctg cttttttagc catttcgatt gcttcaaggg tctctgtcaa agtacctatc
    12781 tggttaacct tgataagtat agagttggca actcccatct cgataccttt tttaagacgc
    12841 tctgtgttag taacaaaaag gtcatcccct actagctgga cacgatcgcc gattcggtca
    12901 gtaagctttt tccaaccgtc ccagtcatct tctgccatac cgtcttcaat cgaaataatg
    12961 ggatatctgt ttacaagatc ttcccagtaa tctaccatct catcagaaga aagttcacgt
    13021 ttgtcagatt tagcgaatac atattttttc tttttgtcat cgtaaaactc ggaagaagct
    13081 ggatcaagcg caatagctat atccttgtca acaccaggtt taaaaccggc cttttcaatt
    13141 gcctgaatta tcacctgaag aggatgctcg ttattatcaa gatttggtgc gtatccgccc
    13201 tcatccccaa cggcagttga aagacccatc tctttaagaa ttttttttag ggtatggaag
    13261 acttctgcaa ccatgcgtaa accctctttc aaagacgagg ctccaactgg cataatcata
    13321 tactcctgaa tatcgatagg agcatccgaa tgggcgccac cattaagtat attagccata
    13381 ggaacaggaa gttcacaagc agcaactccc ccgatatacc tgtaaagagg aagcgagcag
    13441 tcgtcagcag cagccttcgc aagagcaaga gaaactccaa gaatggcatt tgcacccaat
    13501 cgccccttgt tttttgtgcc gtcaagctcc atcatcgcac ggtcaacttc gatctgatca
    13561 atagcagaaa atcctgttat gtgctcatat atctcggtat taatatttgc aacagccttt
    13621 tctacacctt ttccaagata acggtcatca ccgtctctaa gttcaacagc ttcatgaaca
    13681 cctgttgacg cccctgaagg aacagcagca cgtcccaact ctccagttga aagaactaca
    13741 tcaacctcga cagtcggatt accacgggaa tcgattatct cccgtccatg tacatcttct
    13801 ataaacatat tgttctcctt aaaaagtaat attgtttttt taaattaaat atagatctaa
    13861 agccaagaac acagaaacat caccatgcta ataatcaaag gaatttttat atttttcgaa
    13921 aaaaaagtaa tcgatttaat acaaatatta ttgatactct attaacgtca attaaaagct
    13981 taaaacattt tgaaagttaa gtctaaaaat gtcttaatat ttacttgcat atccaagctc
    14041 ggttgaaaag ccaattattg tctttttaac cattattcaa aaatatttaa aatttgatgc
    14101 aaccgtttgc tttttttgtt tgaaataata ttaggaaact ttgaaaaatt acaatatttc
    14161 agagagtaac attggttatt agtttgttta gcatttaaca tatataccga taataaatat
    14221 gtatattgtt ttggtaggaa tgatttttaa aaaatcatca attattattt caatttaggt
    14281 tgcaattcaa agttttacaa atataattat ttgttttgtc aggaggccct ttgctaaaat
    14341 attcaaaagc catcgaagaa aggcttgaat cgattcgttc gggaaccccg acaaagaaca
    14401 ccaaaaaaaa gagaagattt tcaagaggaa ttcttcttgc agatattata ctgattattg
    14461 cgatattttt ttattttaat tcacgtgaag acgaaacatc tctttctaac ggttcggtgg
    14521 ttattgacgg agttaattat cgtatcagct cgcttgttaa taaagatgac atgattatta
    14581 ccgtctcatt gtcttccaga gaaaaaaaac agattttgtt tgaaaacccg gttgcgaata
    14641 ttgttgtatc ttacaagggt gtaacggtac taaaggagta ttgcggaata ggaattgaat
    14701 cggtcgagct tgaatcagat gaagcaagaa atttttttgt gaaagttcct gtttctaaaa
    14761 tttctgatga gatgaaaaag agaggaatcg aaactgaacc ggtgaaacgt tcctttttta
    14821 ataaaaaaca gggtcaaaaa tttctaatac gcggttattt tttgaatagt gaacgcgtag
    14881 agcttgaaat tgtgacagag gttaatttaa aacatgagta gacctgactg gaacacttat
    14941 tttatgcaga tagctgaact ggttgcaaca cgatcaacct gtatacggcg ttctgttgga
    15001 gcggtgattg ttaaagataa aagggtttta acaaccggct ataacggtgc tccccgaggc
    15061 ataccccact gcaccgaaaa aacttgcctt cggactattc acaaagttcc ttcaggtact
    15121 atgcacgaac tttgccgtgg tgttcatgct gagcaaaacg cgattatcca ggcagcattt
    15181 cacggaataa agatagaagg tgccgcaatt tatgttacaa cccaaccatg ttcaatatgt
    15241 accaaacttc taatcaatag cggtataaca actttttatt acaaaaaccc ctacaatgat
    15301 gaactggcgt tacagcttat ggaagaagcg ggagtcgaaa ggattatact atgaaaataa
    15361 tcgcggtgat acttttactt ttttgtgtta caattaatgc ccaggaaaca gaagcagttt
    15421 ctgatccacc acttgaagaa acttctactg aggctaaatc agctggtaca gattcttctg
    15481 tttcaggctc agaatctaca gcttctccca tggctgacca tatgaaagag tttaccggaa
    15541 aaaactttaa aaaaggaatg tcctactatt ttcaaaaaaa attcgagatg gctgaatatc
    15601 atctgcagga agaattaaaa gaaaaccctg agaattattt cgcttactca tttcttggag
    15661 acatattttt aataaaaaaa agatacgatg aggccattac actttttaaa agagctctcg
    15721 atcttagacc cgattatgcc gaaaaccact ttcggcttgg acaggcatat tattacaaga
    15781 atatcggcaa tattgctata agccatttta cagaagcata caatcttaac cctcaaatca
    15841 aatattcaca ttatcatatt gggttaaccc atcttatgct tttgcgagat aaagaaaata
    15901 ctataaaatc atggatgttc tacctttcta ttgctccgga agatcctcag tatgaaaaga
    15961 tccgccgtgc aattgaatta ctgaaagatc cagcttttgt tttgcctcct gtaggtagcg
    16021 atattccgct tgaagaggta ttgcatcttg ggggctcagt tttaaccaag acggagagaa
    16081 gcgacaagga tgacaaagga gctggacatg agacaaaaaa aaccgtcgac cacaaagccg
    16141 atattgaaag agacgatgac ctgtaggggg aaaaatatat gataagatta ccaaaagacc
    16201 ttaagacaca aattgcaatt gctgctggtt tggttatggc agttggatta accgctatta
    16261 ttgcggtatc agttgcaggt gataacgatt tagaagatat tgagaacctt cagaaagaac
    16321 gaatagaact gtttgaaaaa cttcttgaaa cagaccctaa tgacgtcgat acgatgttaa
    16381 aacttgcaag gctttataaa aaaattgaag attacaaaaa cgcgattaag tattacgaaa
    16441 aagttttaga gatcgaaaac gataattacg aggctcttca tgaactgggt tatctttatt
    16501 cacgtatcgg cgattatgat aaagcaatag aatatcttaa attagcacag aagtatagcc
    16561 cagataagcc aaaagcttat aataaactcg ggaatgttta tgatgaccaa gaaaaagagc
    16621 ttcttgccag agatgaatat acaaaggcta agactgtagc acctgaagac caggaagcct
    16681 atcttaatct tgccaagcaa aaactgaaaa aaggcgatgt ttccggtgcc ctttcggaaa
    16741 ttgaagatgg tatgaaaaaa gctccggatt cggctgaagg cttttttaat ctgggtgaag
    16801 tacactataa agcgggcaat tatgacaagg ccattgaagc atacaataaa gccattgctt
    16861 tggacccctc taatgccaaa tattaccgcg ctcttggcga cgcgtatcac aaaaacggaa
    16921 accgcgcagg agcaattgct gcttataaaa aagcgatgga tatcgaccct acagatttta
    16981 aaaccgctga aaaaatcggt gacattaact acgaaggcaa agattataaa gacgctgttg
    17041 caagctacgc tcatgctctt aaatacaatt atgacagtga aattttaaga aaaaaatatg
    17101 atgatgctaa atccaaatta gcatcatcat ccattgttgc tgataaatct tcgggaactg
    17161 gagacggttc cggagacggt tcaggcggcg gaactggttc aggtggtgaa actggttcag
    17221 gtggggtatc cgcaaaaagt gaaaccggtt acggcacatc gggtgaatca gatggctccg
    17281 gaagtcgtga tgcagatatg gattctgcca ttacaggcgg tacaggtgct tcagcaggtg
    17341 cagcagcctc tgttgcaggg gcggcttcaa aatggcgcga aaccggtgat gaacatcgta
    17401 aaaaaagaga atttgatgat gcagtaaaag cttacagaaa agcagtagaa gaagatgatc
    17461 tcgatgatca ttcacattat tggcttggaa gaatttatta ccataacaag atggatgaag
    17521 aagcaaaaaa ctcatttcaa aaagccatac ttgcaaatcc aaaaaatgcg gatgctcatt
    17581 acagactagg acaaatctat ttctttgaga aaaaatatag tgccgcaata aaagcattta
    17641 accaagcaat tgctaatcaa ccagaatttc caaaagcgtt atctgctagg ggtactgctt
    17701 acctgcgact aggagataaa gccgaagcga taaaaaatta caaggcagcc cttgcgcaag
    17761 acgcgaatac ccctggagtt cattttaatc ttggagatat atatctggat tcaaaaaatt
    17821 ttcctgaagc tttaaaatac ttctctgatc atgcaaaact taacccctct agtgctgaag
    17881 ttcactttaa acttggggaa acatatcatg aaatgggaaa caagactacg gctgttgatt
    17941 tttatcaaaa agctataaaa tttgatccca attattatca agcctacttc aatcttggac
    18001 ttctttataa ctccagaagc gaattttcta ctgccgtcac gaatctaaaa aaagctcaat
    18061 cattaaaacc agcggatcca gcaattcttt acgagctggg taaagcttat gaagggctta
    18121 aagatgagcc agccgcaata gattattaca cgcaggctat taaaagaaac cctaattatt
    18181 tcaaagctta tttaaacctg gggcttcttt acaagaaaaa tggcttgtac gacaaagcaa
    18241 tcgatttata ccgaagtgca gtaatcaata atccaagatc ttataacgcg catatgaatc
    18301 ttgctgacac ttaccatgga gccaagatgt acagtaatgc aatagattct tataacagtg
    18361 cgaccgaatt aaaacctggt gaccctgtag tatacttcag aaaaggtatg gtatacaggg
    18421 atagtggtca gtacgatctg gcaattgact cctttaacag agcgacacgt cttgacacaa
    18481 aaaactataa atcacattat gaactaggaa tgctctatta cagaaatgtg aaaaacaatg
    18541 tcaaagcagt tgatcatttt aaaaaggttc ttgcaattaa accggatcat cccaaatcat
    18601 ctaatattca cgaattgatt gatctattat ctaaataagg agttaccgat gcgtatagtt
    18661 attttttcac tgcttctttc tgtatttttc acaggttgtg ttggagcaac ttcaacaaaa
    18721 agtttatcga atcgtacaga gtcaatcgat tctgtaatgt caagggaaga caaagagctt
    18781 cttttgaaat acagacagga aggttttgcc gatcttaaca gttattcggt aataattgtt
    18841 agacctatcg aaaatgaaga tactattgag gagattaccg aacaggctca aagaagatca
    18901 tttgtctctt ttcaaaaata cctaagagaa cgtggtgtaa gaatagattc caatacaaac
    18961 acaaagctat tgaatcttat taacgaaaaa ggatccttaa aacgcattca ggataaggat
    19021 aaatcacgtg tagtttatat ctttgttgtc gaagaggacg gattaaaacg caatatcgat
    19081 tcaatcaaat aatgagctat ctgacaaaaa gctgaactat attaacaccg ttatatttca
    19141 gctttttgtt tattacacac tactcttcaa gttgaatgta atatacaagg tgcttgaaga
    19201 taaacaagct caagctatcc atagattcat tatagatcga actaaaggtt acaccgacgc
    19261 caaattcatt gtcactgatt ttaacacatc tggtaatcat tgccaaggct tcaatattat
    19321 accaaatctc ctctacatct gaaagacaaa tctctactct gataatagac gataccttaa
    19381 aaggaacggg tgatttgaaa aacattcctc cccggctaat attgagtatc ttggttaaaa
    19441 tttctggtga atctgtttca tcatcgtcaa ttaaggtttg agaaaccttt aatggaatct
    19501 cgatctctat acgggtaaac attcgcttat tatcattatc accaacgtat cctttattgc
    19561 gaagccatag ttcgaaacag ttatcaaacc actgttttac agaatcattt aaatcttttg
    19621 tattaccgct acgtactttc ttttcatgat attcaattat ctcattttga attatattag
    19681 aaagcttgtt aaaacatatc atcttgttca tggcaacctt gaagttcttg acaattatta
    19741 tacattttga ataatactaa taattatgga taaatcaact gaaataaaaa aaaattatgt
    19801 cttattatca gagattcacg atgttttgaa aattacaatt ttgcaacaag acactctatt
    19861 gacagtaaaa cacttaatat gttcaatttt tcactgttaa aaaccgattt ttaaataaga
    19921 tgttatcgtt atttatttac tgctattaac agcaaaatct tttcgcttta attcttgatt
    19981 accggagtgg gtttaaatgt attttccaaa aaatgatatt ttccgttcaa tgctgttttg
    20041 ttttcttttt ttctttgcaa caattgcttt ttctcaaaca acaaaatcgg ctagaatcaa
    20101 ggcaatactt gatgccgaag ataatgcgga tacacgttac gaaattgagc aaattgtaaa
    20161 aacctgtaaa tatcgttatt cagatcttta tagtaaactc gaaaaagggg acaaaatcac
    20221 catatttata gaccctgctc atggtaaact ttcgaatggt caatggcaag gaggcggtgc
    20281 gacttttaga cagagtacta ccgaaaaacc ggaagagttc tactcgatac ccctttcccg
    20341 tgaactttat aaacttcttg tgtcaaataa atatatacgt gttgccacca cacgcgacta
    20401 tatgtcagtt ttaagagaag agtcaaatac gtataatgat atatccttta ccgaaacgat
    20461 taatcttgca aacaaaacca actctttttt gattgtatca tcacatctta ataatatatc
    20521 gcccattcat aaggctgatg ggcttgttaa tatgtatggt atacatatta ccaaggatcg
    20581 taatggttca agatatattt ctcatattac gtcagttcaa cgagggtttt taactttata
    20641 taataaattt gaccctacgg gttttagccg cagaatagca gaacgcacaa aagagaatca
    20701 tatatcgatt aacatgtatc caaacgcttg ggacgacagt atagttgcgg atgaccgatt
    20761 ttcctatttt tctgagtatc ctttctctgt aatatacgaa tcaggtttca tttctaatcc
    20821 ggttgaagag agatttttaa ggataccgga aaatcagaaa ttgatagctc aacagcaata
    20881 tctggcgctt gttgcatctg ttgcggatat ttttggagta gatatatcca acggcagttt
    20941 acgaaaagcc ttcgatcagc cagagaatct tgtaaacgac atcattcttg ctcgactggc
    21001 tgtatactat atcaaaaaaa atgacaggaa aaaagctata ccaatttgta actatatcgc
    21061 agaaaataca aacaaagata taaaggtctc taactattac aagtttcttg caacccgctt
    21121 aagctcagtt gagacaaaca ttgccaatgc ttatcgtaat cgtaaaaaaa caaaattatt
    21181 caattcctat ttaagaaagg ctcgaaaagc agttggtggc gctcatatat tctcttctac
    21241 tcgtaaatct ataattgcat tgcaaagagg aagaaagggc ggacattatg tagcctctgg
    21301 cagaagatca cttccctatt ccaaaccgct tacaactaaa accgaaaaac actctgtaac
    21361 tactccgttt cttttaacaa taaaacctgg ccaaacatta aaagaagcga tagatcagtc
    21421 tatcgcacct tcagataatt ataaaaaaaa ggtttatagc gcattttcag atgtaaaagt
    21481 aacaactgtt aagtgggtta gaaattattc taaaaagaac aagaagtata tttgggtaaa
    21541 aaaaagatat aaggttaaaa aaacttttaa agaaggtatc tacgtcgtaa aattggataa
    21601 aaaatttaac gttatctctg tgaaggaagt aaaaagagtt acttttgacc caacaaaata
    21661 tcaaaatcag gaatacttta aaaattcctg tctttcaaat gctgtcaaag acagatcact
    21721 gtaatattta taacgggaag ccatacaact attttgggga ttatcttcga agtaagtatg
    21781 gctttcgaat ctttaaactg cctataaacg cgtttttagg ttgcccaaac agaatcaacg
    21841 gtcatggttg cattttctgt tcagaatcag gttccgcctc gccttctatc ggtaatcacg
    21901 aatctatata taagcagatg caaaatgcga aaacatcatt taatcgcaca tctgttgaaa
    21961 cccgatatat tgcctatttc caggcatata caaatactgc tggtaacata aatacattga
    22021 aaaagcttta cgatgaggct ttgagcttcc cagatgtgat gggattaatg ataggtacac
    22081 gtccagatga gattaatcca gaaattacag atcttatagc tgaatacaat gttcctgatt
    22141 ttgaattgtg ggtcgaactg ggtatgcaat cggcacacga tagaagcctt gactatttaa
    22201 acagaatgca cagttttagc caaacgcgtg attcgatcat acttctttcc aatagaggta
    22261 tacaggtctc tgttcatatt attcttggca tccccggtga aagctgggct gatatgatgc
    22321 aaactgcaga tacaatatct tctttgcctg tggctggagt aaaaattcat catcttcatg
    22381 tattaaagaa tacacctctt tacagtatgt acacaaatgg tgaggtgaaa ctccttgagt
    22441 ttaaatctta tatatctatt ttaactgatt ttatcgaaag attaagaaaa gatataacta
    22501 ttcaccgtat agccggtgat gctcttgaag atgttttagt cgcacctaaa tggggattag
    22561 aaaaaggaac agttcaacaa aggttaattg acgaatttaa cagacggtgt acttggcaag
    22621 gctttttata taaagattga gaggaaaata agccgaattc tgtctaagta cggttatcta
    22681 tcttgcatta acattgctgt tactgtcttt gcggcctacc cgtattcaaa agcgagcaac
    22741 ttcatgaata cctatttggc cttgcaccgg atggggttta cactgccacc gaaccttgcg
    22801 gccggtgcgg tgagctctta cctcgccttt tcacccttac ctaaaaacat ctgcatgcag
    22861 atgtttttaa ggcggtatat tttctgctgc actttccgtc gcctcgcggc gccctgacgt
    22921 tatcaggcat cccgctctgt ggtgttcgga ctttcctcac ttcatccgca atagcggata
    22981 atcgcgcaac cgtaaattcc tctcaatctt tagtacaaag ctgaattatt aataaataat
    23041 agcaataatt tttttatgtt gattaattaa ccagttttga ggattcagtt attagataaa
    23101 taatgtaaaa caatcaccat aaattcaaaa tttgatttta tattactaat attttaggaa
    23161 aaaaatgctt actgttgatt gtataaaaaa aaatttctgc gataataaag ttctacagag
    23221 cgtttccttt catattccac agggacaggt agttggtttg cttggtgaga atggagcagg
    23281 aaaaacaacg ctgtttcgta ttatttgttc actaatttca gcagatgaag gcaatgtata
    23341 tatcaatggc caggctcttg aggaagacag gatttgttac ccatctgttt tttttggtgg
    23401 cagttcaggt ctatattcca gacttagcgc cagagagaat atcacatatt ttgcaaattt
    23461 aaacggagtt tatggagatg tactgaacaa taaaatagat tatttttcaa atctacttga
    23521 tatgaatgat tatattgacc ggaagactct tcaattctca catggaatga aacaaaaaac
    23581 cgcactggta aggatggccg taataaattc acctctaatg cttttagatg aacctactac
    23641 cggaatggat ttcccgtcaa ttgtttcagt tgccgacttc atacaacatc aaaaaaatga
    23701 atctaaaact atactttatt caagtcataa tataagtgaa ttatccagca tatgtcaacg
    23761 ggtcattatt ctgcataaag gtgttatcaa ggcagatata gaccttatta aatacagtgc
    23821 caataaagat aatattgtta aaaaggcata tttaaggatt cttgaaaaat gattaaatct
    23881 atttttatta tttttaaaaa agagtctatt gacgccatac gtgacgtcag atcactgatc
    23941 attactttca tatttccatt aatagttttc cccgccgtaa ttttattaac agggagtaaa
    24001 acggatacga tttataatat ttccgttagt ggtatagatg agtcaatcat tgcccactta
    24061 caagccgatc ctttgctgtt agtctcaaaa aatgagatta cggataaaaa tgcttcactt
    24121 ttcaattata gaattgatgc atcacttagt atgtataata aagctaattt taaagcgatc
    24181 gaaattgagt ataataacag ctacaagcga tccattgaag ctttagagta tttgaaagta
    24241 aaactgaccg aatatcagat gaacaatatt ctcgaaaatc ctgatataaa gcatactgga
    24301 tttattgtca ccttcatgcc gattcatgat atatatgaag gagcaagtat gtttatttta
    24361 tatactcttc ttcctgtttt gataataatt atttctataa catcaccaat tgcggtcgcc
    24421 gccgatgttt ttgctggtga aaaggagcga aattctcttg aattgttgct ttgtaatcct
    24481 gtttcaaaat ttgctgtagt attgggaaaa tattttactg tttttctctt tggatttatg
    24541 ggggtattat gttttcttgc aggttttttt atttcagtgt ctattgatag cactttttta
    24601 gggatatccg aactgattat aacttcaaag cagatatctt acagcctgtt tcttgttgct
    24661 atactctctt ttgttacttc atctatagaa gtttgtataa gtataacagc aaaaagtgta
    24721 aaagaggcac agctctacat cttgccactt acggttattt tctctgctat caattatttt
    24781 tcggataaaa gttttttttc agtaactgat accaatattt catcaagact tgattatata
    24841 cctgtattaa acataatgac ttccatgcgt acagttatga tgaataacga tataaataat
    24901 ccccttgcta tattactcac gaatatagta gcctctataa tagtccttta tatttcaaaa
    24961 ttattattaa accgtgaaaa aattatatac aggtcttgaa ttattttctt gacattatac
    25021 tatttttaag tttgttttaa tcaaaagtct gaatttaagg agatcctcaa tggaaactat
    25081 cttgcttatt attcttgtag ttattttgat ttggtttatc atgagaatgc tttaattact
    25141 gcatcacata gcttttaacc ttgtccattt ttcttaaaat ggacaagtaa atagctttag
    25201 aaacaccaaa ctctttcatt ttgtccataa atgtatcttt taatttgtct atcatttctt
    25261 caagatcgga atattcatca attttagaag caaaatcatc aataattgca tggaatacat
    25321 tgactcccag aatttcatta ttaaaaagag taagattttc catgaaaaca taatttgaga
    25381 tattatcttc ctctttgtct tcgcaacact tctttatcag taattcaata tcagttttta
    25441 aatcttcaag ttcaagtttt agaatagaca ggtaattgct tttctttttt aacacaacat
    25501 tacctccggt ttatatcttt tacacatcta aatccagatc tattgtcaga gtgaagatct
    25561 ggcaaacctc cataagctcg agaactgatt gtaatttcat cagcactact tttgtaatca
    25621 cccccacgaa cgactttaac ctgttgtcca aaacgtattg aactacaatt tgctccagga
    25681 tatttttcat accatgaaga tgtccattcg gcagcgttac cacacatatt aactactcca
    25741 tagaatgaaa cgttacgctc tttataataa taaacaggaa ggagacctct ttcaaatgga
    25801 atgttggtat tattttccca aaattcattg tagttaacat gattatgcag gtcatttccc
    25861 caaggatatc ttgttttaaa tggtttttct ataaccccta aattggtaac tgttttttga
    25921 gtagcctcta caccattagc agctctttcc cattcaaact ctgtgggaag tcttttacca
    25981 gcccacttgg cataagcctc agcttcataa taggtaacca ttaccgggaa atcggccatt
    26041 ttttgtggaa actcgccatt agtccatgat atgggtgcat ttactgcgtt ttctttgcaa
    26101 aaaacaagat aatcgcgatt actgacttcg tatttgtcta tataaaaact ttttataatt
    26161 ttattatgta ctggtgattc atggggttgg cctagatcag aaccaatcag agtaatacca
    26221 ccactcacaa gaaccatctc tcttttatcc acttccgaaa ctatcgattt atcataactt
    26281 tttttttcac tagtaaatga gtcattataa accggaattt ttttttcggg ttttgaatgg
    26341 aaagtaaagg tatcaccggt ttttaacttt tttaatacta ccgctgatgt aatcgcgata
    26401 tattcatact tgtaatttgt aaaactgtta actctgctac ctgattgaat tgtaacttct
    26461 ccagagttaa cagaattcag gtgaatatta taatgcctat tggtcagtct tttttcagat
    26521 tgaaaaacaa acctgttttg atcaagaatg cttttaacga caaaaacttt ttccggctgc
    26581 gaaaagagga ttactggtaa aaagagtaaa attgcaattt ttttcatatt atcccacctg
    26641 gtaaatcttg cgatccggca ttcttattgc ggttagatat ccgtaataga ccgcaccggt
    26701 atcaatacca attatattct tattttcgtc aatataggga gtattcctct cttttgtaat
    26761 aaaaaaagtt ggagtatgac caaagattac cgttttcttc catttttttt tgtcattaaa
    26821 aaactcttta cgtatccaca taagatcaaa ttcatcacac attacaggat tttcataatt
    26881 agggtttatt cctgcgtgaa cagctataaa atcagttctt tcaacatagt gaatacccga
    26941 aaagaggatt tttttatggt gttcgggtat tacaaatgat cctgtattgg ccatataact
    27001 atcgtaagtc gtctgaccac cgttttgata ataaatggcc ttcgattcat ttttatatat
    27061 tgtatcgtac agcatctgct catgattccc cataatgaag atcgttttat actttatgga
    27121 taatttgtac aaatactcaa caaccttata agaatcaggt ccacgatcga tgtaatcacc
    27181 taaaaaaatc agttcatcat tttccggttc aaaatctttt tcaattattt taataagttg
    27241 caataacttg gaaaactcac cgtgtatatc acctattaga tagttcattc agtcgagttt
    27301 ctccagtatc ttatcaattt cgaggggaat cgagagataa gttacattat caaaatctac
    27361 tgggtcaaca gattttttat gtattaccag aagtctctta gaagaatgaa tcgcataaaa
    27421 atcttcaggt gattcataaa tatctactat agaatcgtaa tcaactacaa tatgtgttac
    27481 ttttgaatag ctttctagca gattcagttt ggatattgat ccaacaatta cattataacc
    27541 gtttttggtg aaattttcag acaataagag ctctgttatc ttgtctgcaa aactgaccag
    27601 aatggttccc tctttttttt caaggggttc tggaatgtca tattttcgaa catcactgat
    27661 agtctgcaaa aaggaaagtg ttggaaacga gataataatt tgaagtcgac tatagtcgga
    27721 aaggttatct atgtggatca ttccgccaat gcgtcttata ttttcaactg cggtgtttag
    27781 tccgattttt aatccatggg attctttggt tccgatatcg cttttatcat ctgtaataaa
    27841 caaacgcaaa agtgttttta tatctggcat tttttctgac aggtcactaa aaattaaaga
    27901 aattagcact gaagagccct cttcgctaat tctggtttct actgaggtat tatcagtaat
    27961 aaattgtgaa atagaaaaaa atatatgata aagtgaatct ttaaacacct cctttgatgt
    28021 ggaaatctcg atgtttttat cgccgcttat taccatcctt ttaaaaacga caagttctaa
    28081 aaaatcataa cgtatgactt ttaaaattga gtatggagaa aatgtctgat aagtcttctc
    28141 attaatcaac tcgcttacat tcgttacctg ctctgctatt aaaacagaaa aattaacaag
    28201 agaatttagc acatccacaa attcatgagg cggattatca ccaaaccagg atggggcatt
    28261 tttaatcaag gcgtctatat ttctatgaat tatactcagg aaaagcgagt tgcgaatctc
    28321 aaaatcttga tttaacttct caattgactt tttaattata tcatgatatt ccatatagca
    28381 tatgccaaac ctttgttcca agagccataa ttagctaact tttagtaata ctatacccat
    28441 ttttgagaaa aggtcaataa ataataaatt ctgaaaaaca gatatttaat tataaaagta
    28501 aagaattctt tataaaaaat acagtttttg ccgttagtta agtcagttag ctttattcaa
    28561 atagtgttat ttgttatttt tcaattattc gtatataatt ctattattgt agtctgatat
    28621 ttattatttt taaatattta gaacaaaaca tcatatttgt accaaggtta attaaaaaaa
    28681 actttacata atagttaaca atgtcaatat gtcagtcaat caactggagg attttatgga
    28741 catatctatc cgcgaacatg gcgacaataa aattatcagt gtaagcggtg aagttgatct
    28801 ctacaatgta agtgagctga aaaatgcatt atttagttta acagacggct cgaatagcag
    28861 tgttgtagtt gatatgaaag aagtaaacta tatggattct tccggtatag gagctcttgt
    28921 ggctggtcag aaaaagatga aggctcataa tggctcgttt gccttaatga atattagtga
    28981 tgacgtactt aacatactaa aacttgcaac acttgataaa ttttttaaaa tatattataa
    29041 cgaaagtgaa cttgtttaat tctactcagt acgtttaacc acaacaaggg tcatatcgtc
    29101 gtgaggggga gtttcccctc gaaattcctc aacatcctct attattgttt tcacaatttc
    29161 ttctgcgctt ttttcacaat tttgcattag tattttttgc attcttgttc taccgtattc
    29221 ttcgcgattt gagtttcgca tttccgtaat accgtcggta tacataacaa caatatcacc
    29281 tacatcgaac cgggttgatg actgtaaata ggatgcttcg tctactatac ctattggcat
    29341 accttccaaa gttgtgactg tacaatgttt ttcctttgcc ctgtagcaaa aaagaggtcc
    29401 gtgaccggca tttgtaaatg acagagtccc acttgcacgg tcaaaaatta taaacatcat
    29461 tgtagcaaat ttatctatag caaaatcagc acttaaagag ttgttcactg cagttacaat
    29521 gtttttgcat tgaacagaag tggagttttg atcaaccgaa gatacaaaaa cagtacgaat
    29581 catcaccatt acaagtgatg ccggtacacc tttaccagag acatcactaa tcaaaacacc
    29641 aactctggaa tcatctatat cgatatagtc aaaataatct ccccccaccc ccttcgcagc
    29701 tctagtatat ccctgaatct gtacaccacg gctgttgtaa aaacccatcg ggtttagacc
    29761 ttcctgaatt tctttggcaa gctcaagctg ttccgccaaa agccggctct cgatttcctt
    29821 gttcttagcc tctttcaact cgagagtcat agagttaaac tcactagcca aacgtcctat
    29881 ctcgtccttg gattttattg ttatcttatg atccatatta ccagttgcta ttatggcaac
    29941 accatttgaa agatgtttta gaggattagt tgtaatgtaa gataatataa aagaagcgat
    30001 tacagaagca cctataaata ttaatgctat aacaataatt gtgttttgaa gtttttttat
    30061 ctgatcacga atcaaggcgt cagaaaagcc aagacgtata atcgcgatac ggctatcgtt
    30121 caatctgcta taaacaggca ttataaaatc gtaaattttt ccgttttcat cctgattatc
    30181 ctttatctct tgctctaaaa cattttttat cgtgctattg ttaatagctt tttttaaatg
    30241 ttcatcttta ctgttatccg gaattttatc aggattgtct gagattatta cgctaccctc
    30301 tggatcggtg acataggcgt aacggaaaga tttgttctgt ttaaaaatat ttattgtatc
    30361 ataaattaca agatcattat cgcctaacga agtttgagtt actgcagaaa aagtattcat
    30421 cataaggcgg gcattgtcct ttatattgtc tgttaacaaa agagtctctt tttgaagaaa
    30481 ataatatgcc attgatgaaa taatagttac ggttagaaca atgattgcaa ttgaaaattt
    30541 aaatttaagg ctaaaaataa cacgtttctt tttactgttt ttcagctctt tttttgcatc
    30601 cgcaatgttt tttttatttt tcatcataag ctcactgttt cattaaaaga agacaatttc
    30661 gtatattatt tcttgcttta tcatggttgg gatctatagc aagtactttt cgccattcgc
    30721 ttacggcttc agcaaattta ttctgagagt agtagtttat tccggcaaaa tagtattggc
    30781 gaaccagttt tttttgttcc tcactaagtg tagcagatct tttcttagcg ggattcccag
    30841 caagacgtat ttctgattca attttgttaa catttattat cgctcttgtg ttttgtggat
    30901 cattattaac aacccattta aactcgctta atgctttttt aaaatctccg ttacggtata
    30961 attttatacc ccgattatat ctctgttcaa tttcggccgg tgatacggtt atacgaggag
    31021 gtggagtacc cgcaggtatt ggtatttgag gtttagaagg tttagctgac gcaataagat
    31081 tatcaatatc tggataatct ggagaaattg attttataat ggaaaaacgc cttaatgccc
    31141 taggataatt tttttgtttg agcatttttt tcccttcttc tatctgttct gcggcaaatt
    31201 ttttataaat gtctggattc agtttcaact ggcattttag caaatattca gtagcaaaag
    31261 tattgtttgg aaacagatta agaatacgtt cccactgatc cagtgatttt cggtattcct
    31321 gtctttcata atatagtttt cctgaactta tgagagtcga tacaagagaa taatatggag
    31381 aactttcatc aattatatct tctttctctt gagcaagagc ttctttagaa gttttaacat
    31441 aacgtcttgc ctgatcgttt gccggatcaa tctctattat attttcaaag gcggttacag
    31501 cactctcgta gtctccctgg gctagataaa gaagacctgc ttgaaactgc ctgttaattt
    31561 tttccagatt ttgctgacgt attctttcgt tgtactcttc ttcaagcttg tcaagacctg
    31621 ctaatcgaga ctttgcatcc ttataatctt ctataagaga aattaccatc tcgtaatctt
    31681 cgcgtgcctg atcaaatttg tattcattac tattttgtaa acccgaacta tagtaacgtt
    31741 cagcctgtag tcttttacgt tcaagcttct gttcttcttc cagaatatca tctattttgg
    31801 cgatatagtt ttcggcatca gggttgccag gatctaattt tagtatctct tcaaaatcgt
    31861 gaagcgattc ctgatatttg gtctcttcaa agagtctgat tccgttttcg gttaaacgtt
    31921 taatctgttc aaaacgcata cggctctgta aagcaagagt ggctttgcgt ttaccttctt
    31981 tagcgatatt gtcgtccgca aatatcacga gcattgcatc ccattctgtt attgcgtttt
    32041 catattcttt attttccata aattctagag ctgctgcgta atgatcgtcg tatttctttt
    32101 tcatttgagc atgcaatcgt tgacgttcta actcttgtaa agcatcagct ctcatacgtt
    32161 cgacacgagc gcgtaaaatt tgcatttcag gatcacgcgg agttattcta taagcagtaa
    32221 taaaattttc tattgattta tcagcattat caaggctttt ttgggcttct tttttttcca
    32281 agtgagttcg tgctatgcgc aaaaactttt ttgatcgctc aaaagcaaga tcgcgtgttc
    32341 tttttcggtt ataaaccatt tcaactttgt gttgaatctg tttattgcct ggtgactttt
    32401 caagagctgt aatccaatca actatagctc gttcgtattc accagcttca tagtaacgtt
    32461 gagctctatt atcaagttct gaaacagttt gtccgtaaag attaaaaaaa aagagaatca
    32521 atacaaagaa aagataatgt aaagactgtt ttttcatttt atcttatcca aaaaatgatg
    32581 taaaaaaata atttttggat aatatccaca tttgcttgga ttactcattg tgtactgtaa
    32641 tcatatgtaa aattttccac tgtaccgatt tttccttggg agtaaacaca acagcgtctg
    32701 ccctaatcat tccgatatac tcatcattat cacgagcata tttatcggca gcaattgtta
    32761 cattattaac aaaagcaacc tcatcaccaa ttatctgttc acctttgaat acaatattca
    32821 tgatttgaat gtttttataa taattgtctc caaatttctt ttcaaaatca tctttagtcg
    32881 aagattcggt aaatacattt ctgtatagtg agtaatcgtt cttctttaac gcgacctcga
    32941 aaaccgtaaa gagtttcttt atcttttcca tatcatctgt atctggatta ttagatttct
    33001 gtttagtgca atttgttaac aacaaagcaa taattaaaaa ttgcacgatt ctattcaatt
    33061 tcattatttt ctccgaaaac ctctgcagaa aatttttcaa ttactgttga ttcatctttc
    33121 catgattcag catcaagacc agctttaaga catgtgtgcc ctagaaaagt ttccagatcc
    33181 caattatttt cggtagccac ctgcggcaaa agaagacccg aactatgatc tttagtaatt
    33241 attattccat ctctaccaac aacaatccga ctgatatctg taacttcttc aatttcagaa
    33301 agtactgata tttccaggtc aatttgctca aattcagccg atgttaaagg taaaaatctt
    33361 ggatctttga aagcggctga ataagccatg tcttttacag cctgtaaaag aggttttatg
    33421 ccgactatat aaccgataca cccacgaagc gacccattta aatgaagggt tacaaatgct
    33481 ccacattttt tattgaagat aagatcgctt gtatcgattg actctgcaga atatggcatc
    33541 tttatgccta gttgtgaagc aatagttaat cgtgcaatct ttagaagaga ctgttttaca
    33601 ctcttggaaa tatccattat agacctctct taataaataa cagcagccat ataaccgact
    33661 accctgtttt tttcacctga agcatcaccc gaatttttgt acataagctt ttcacacgtt
    33721 tttgccccaa attttttcag aagtttaaat cctgtataga tagccgcgaa gccgcaggct
    33781 tcactcccat tttgaaccga atgatacaaa gtatcttcgt taaattctgt tataatctcc
    33841 atggtagtat tatctatctg aaccgcttca tcgtatcgat gaaaatgaga aagatcggtt
    33901 gagataacta caagaggttt ttgtttctca tcttcgtata ttgatgctat atcagacgca
    33961 attgaggtgc atattgaagg atcttgcgat ccaattaata ctggaattaa atcagcgttt
    34021 gggaaaacgg tctgaagaaa aggtacctga acctctatag agtgttcaga atcgtgcgca
    34081 gcttttaaaa aggtcattga gctcttttgt gacaaatctg ctgctgatac gtcaatttta
    34141 acatctccta atggagtacg atacagaccg tcaggtataa ctgatccttt ttcaaaagaa
    34201 tatctatgtg atggtgctaa aacaataatt ttggatggtt tgcaatctga tgaggacatg
    34261 taagaataag ctgctatctg tcctgaatag acatatccgg catgcggaac aattataccc
    34321 ttggcagttg ggtgttttat tacagatgat gcgttttcaa gataatcatc tatctcagcc
    34381 gcaagtttag ccttatccga aggataaaat gttccattat aagctgtttg cctgatatac
    34441 atttcagatt ctcctggtaa cattatgaaa aatttgctta gcaacgttaa cttttgtgtc
    34501 cattggaatc aggatttttt ctccggattt acagtagatg gtaacttcgt tagtttctcc
    34561 cgcaaaacca accccttttt ttgatatatc attaagacaa ataaaatcaa gagatttttg
    34621 ttttagtttt gaaagagcat agttttcagt attatgtgtt tctgcggcaa aaccaacggt
    34681 aattatgttc ttatagtttc ctgatagttt caattgggca atatgcataa gaatatctgg
    34741 atttttctcg aagtttatat taatacggtc acaatttttt tttatttttt gatcagaata
    34801 gttcaccgga cgaaaatcag acggagctgc cgccataaaa attatcatat cggaagataa
    34861 ttctttttcc atagcacgaa gaagatcatt cgtagtttcg caacccttac attcataagc
    34921 tttatttttt aatagattat tatctataac tccatgaagt aattttatac ggtttgaatg
    34981 tttataaact tcatctgcga ttgctattcc cattttgcca gatgacggat tcgaaataaa
    35041 cctaaaagga tcaatccatt cccgggtagg tcccgcactt acaagataag aaaagtcctt
    35101 gtcactagcc attctgattt tccaacaatt caagagatct ttttacaatt atatttactg
    35161 gagccaactt tccatatcca gtatcgccac acactacatc accggtgtca ggttcaataa
    35221 tggttatatt ccgctttttt aagactgaaa tattatgttg aacggctgga ttagaccaca
    35281 tatcaggatt catggccgga gcgataacaa tgggagtctt catcgataaa aaggtagtag
    35341 taataagatc atcagcaatt ccagatgcgg cttttcctat aaaatttgca gtggcaggag
    35401 cacaaacaaa aatattggcg ttttctttaa gtgcaatatg ccccatatta tagacatcgg
    35461 gcaaaaaagt ttcatgatat actgggtttc cggataaggt tctaaatgtc aaaggtgaaa
    35521 caagattaag tgcgtttttt gacataacca caaatacatc atgcccctct tttacaaaaa
    35581 gcctagtaat atcacaagcc ttataggccg ctatagaaga ggtaactcca agtacaattt
    35641 tcatattaac cttctgaaga gcttttcaaa atctttacgg ctaatatcgg ctacgaatgg
    35701 gcgtccatga ggacagcgta acggataaga tccggaaaat atttttacaa gcaatgattc
    35761 agcatctatg cgactcatcg ggtcaccagc ccgtttggca ctgtgacagg ccactctttc
    35821 ggccgcatgt tttttgattt gtataaaata atcttcggca ttacggtcaa gagacaaatc
    35881 tgaaatagat tgcgtaaatt cccgaataca agtaacagcc tgtgatggat caagcgtctc
    35941 ggggatgcct ctgattacaa gtgattcttg tgaaaaaatg tccagatcga atcccatgga
    36001 caatagaaca tcaaggttat cagaaacaaa agaaacttcc tgaatatcca tatttacctc
    36061 ttcagggaaa atcaactttt gaacggaggg cttttcttcg ttctttataa gcctatcata
    36121 aataattctt tcatgagccg catggaaatc cacaattttt aaagactctt ccttttctac
    36181 cagaagatat gttttaaaaa gagtaccaat ataataccag ttatgaagtt gattataaat
    36241 atctcgacca tcattaaaat aacttacggt tttcgatggc cctgttttta atggatcatc
    36301 ggtataatca ttcataattt cacaattacc aagattatta aaaccgccat ttatctcatc
    36361 gtttttgctt attcgcgaac cttcgttatt tttttgtgac acattttttg aattcagatc
    36421 gttatcagca atttgccata aattagattg atcgtggtct gcttcattct cataactact
    36481 tgaatggagt tccctttcgc taatgttatc atttttaaaa ttggtaaaaa ctgaggaatc
    36541 cagatgtatc tcgttttgac caagagtttt tttgacaaaa ccatatatca gactgtcgat
    36601 atagcgagtg tcaaagagtt taatttctct tttacaggga tgaacattaa catcaataag
    36661 atcagggggg atagtgatga aaaggaacag aacggggtgt aatccctttg gcaacattga
    36721 atcataagcc ctgttaacat ggaaggaaag atttttatac tctactggtc gaccgttaac
    36781 aaagagaaac tgcatagacc ggctcttttt taatccttcc agagatgata taaacccgtt
    36841 aatattaacc ttgagatctt gaacggaccc ttcaagaagt ctggaggcct cattcgaacc
    36901 ataaatatca gcgagccgtt ccctaatgtg ctcacgcgca ggaagagaca tcaatacctt
    36961 gtcttcattt tctagcgtca tcacaatagc cggattagct attgcaattt taataaaact
    37021 ttcgcgtatt agtcttattt cggtagagcg agcttttaaa aatttttttc tagcaggtgt
    37081 gttataaaag atatgctcga caataacagt tgttccaact ggcccagcaa aaggtaatac
    37141 agaaatatta tcgtcagata caagttttgc gccctcatcc aaattatgtg gacggcttaa
    37201 aattgtaata tgagaaacag ctgcaatgct tgataaagcc tctccacgaa atccataact
    37261 atatattgat tgaatatcat caacagacgt gatcttggat gtcgcgtgtt ctgttattgc
    37321 aagtttgagc tcctccggaa gaatgccgca tccatcatca gttacaacaa ttcgacgcat
    37381 tccgccgtct tcaattttga cactgattct acctgcccct gaatccgcgg agttctcgat
    37441 aagctctttg ataaccgaat gaggtccttc gataacctca ccggctgcta tcatccgctt
    37501 aacatgttcg ggaagctcgt gtatactggg aatagttttg ctatctgttt tcaattgtta
    37561 gctgagacct gattccatcc tgatttgtag cttcaaaacc gaaacttaca ctctcctcat
    37621 caaaaccggc aatctctttc aatgtttcac tattatgtat tgaagaccac ctgacagcat
    37681 caaaactgat aaaaattcgt gagttgcgtg atattttata attacttcga ttggaactat
    37741 tctctgggtt tttaatcata aagtatatgt ttctttttgc tatcatattg atatcactat
    37801 tttcatctac cctaacataa tcgttattaa gtactattct taagggaata cggtcgcccg
    37861 cagtaaaata aagtacgtaa tagtcttcat cggccaattc accatcaaaa gagtaaatgt
    37921 tgctatagtg aatcatactg tcaggattca cctgtttaac aattatggaa tcggttaatc
    37981 cgcacgatgc taacattaaa gctaatataa ttaaaaaaag actttttttc ataatacatt
    38041 cctctgcaca caattcttta tatccatcaa gaatcgaacc atgctatttt tttattctct
    38101 tatttcaaga ttaaattaaa atcaattatt gagaattatt tggttacggt taataaactc
    38161 tcctttttga atatattttg tataaattca taaaaaagat aaaatttcag gtattgattg
    38221 caaaaataga tatatataag gctatagact agttattcca tataatgata caatttttgt
    38281 ctaatcaatt acaacaaaac agatactgga aagtttattc attcttttta cggactatta
    38341 taacaaaata ttcataaata tcaagtattg atgccaaaaa gcacttaaag gctgtttttt
    38401 aaataggata taaaatttga cttgatttat ataatacatc tgttatagtc acgttattct
    38461 acttttctaa taagggaaat tatgtctagt aaaaatgata ccgaaatcag ccctgttgtc
    38521 gataaatcga ctgtaaaact taaacgcaac cgaaaaaagg gttttgactc ttctttagct
    38581 tggtatcttg acaagattaa caagataaaa ctcttaactc gggaagaaga ggactattac
    38641 gcgcgaaagg ctcttagtgg agatcctgaa gcaaaggaga tgttgatcaa gtctaatctt
    38701 cgtttcgttg tttccatagc gaagaaatac cagacgagcg gtatttcttt tttagattta
    38761 attaacgaag gcaatatggg gcttatcaaa gccgctgaaa aatttgatcc agataaaggt
    38821 ttccatttta tatcatacgc ggtatggtgg atcagacagg caataattct tgccatttca
    38881 caaaagacat cccttatccg tattcccctt aaccgaaccg ctgatcttca gaaaattgaa
    38941 aaagttcata aacgtcttga acatgagttt ggtcgggaac ctacacctca agagatatct
    39001 gaatttcttg atatggataa cgaacagatt aatcacctaa ggaatatttc acaagattat
    39061 atttctctcg atactcctta cggtgattcc gatgatacaa caatgattag cactgttgag
    39121 gatatacata ccgatgcccc ctacaaggat cttattgatg aatctttacg tgaagagcta
    39181 tcatcagtgc ttgacacttt gactgtatca gaaaaagata ttttggtaaa gcgatacgga
    39241 ttggacggca acaaacccat gtcgcttcac cagatcggag aacagtacag cctatccaaa
    39301 gaacgtattc gtcaaattga aaaaaaagca atacgaaagc ttcgccatcc atccagaagt
    39361 tttaagctta aaagttacat tactgattga tgttcaaaaa aataaaattt gttgacgcta
    39421 ttaactgaag ctctgtaatt aacagggctt ttttattact gatacgaggc atatgtatga
    39481 aatttttatt tttgggaatt gttcttggag gattattggc tttcattccc tactccgaaa
    39541 tagataacgg tgcccgtgtt actcctgaat ggtttggttc cgtagaaaaa ataagatcca
    39601 tagatccgtc caacaatatt aataaaacca aaggttattt cctttatcct tctcttgacc
    39661 acgcttatct tcttaatgca gatggttcgt ttcataagga atattcaggt gatgaaaact
    39721 cacgtttcgc gttttcaggt gacaaagaat attatatcca gttcgaaaaa gttggttcac
    39781 atatatcgtt tctaaatcga actggtgcca aattctggat taccgagacc ccacagtacc
    39841 catatctttc acattcagct aaacttgtcc ttcttctcgt ttctgatctt cacacaatct
    39901 ttttaaacga taaaaacgga aaaccggtgg gtattcagaa aattgagggg cgaatgtgca
    39961 ccaatatttc attttccaaa aatgattacg cgatagccgg ctttatgagt tcagagtatt
    40021 atattgtgga tagctctgga aatcttgtat tacatggtaa aactcctgaa ggaacaattg
    40081 ttaaaagttg tgtattaagt gataactcct cttacgctgc gattcactat ggaactgtaa
    40141 acgaagacgg ttgtatgata attaatattt ctaatattcc tgacggcgat tttgagctat
    40201 ctgtaaaaat ggagagcgtt cataaaacac gaactggaat tgcaatctcg gataccggga
    40261 cattgcatct gcttgacgga ctatattacc gaatgtttga taataaaggt aatccgttat
    40321 tcgcgaaaga gatcgagcca cagtctcacg gtcatgcaca gatttcgctt tataatggtt
    40381 tagccattgc aagttatcga ctctttggtg gtaagagtat cttgagtatt tatgataacg
    40441 acctaaatat actgcattct gaaacatttg aagatacggc tttgaatatc gaaatcacag
    40501 gtgatgttct ttttgcagca ggtacgaaaa atctctcggc gtacagcctt gctcgataat
    40561 gttcaaggtc tcattcaagt ttttgaaaag gtttttatta ctgtttttta tagctcctgc
    40621 cataacgatg tttatctttc gtttaagaac cggctctatt tttttttggt attcgccata
    40681 ttttgtgtta ttaagtagtt taagcatact gtaaatgcga aaccgatcca tggcggtgac
    40741 tgatcgggat agctgatcat ctgctcctcc atattttatt attgtctgtt tgtcgattag
    40801 ccctatttcg taaaatggtg ttattctaag ccatagatca taatcttcac aaacaggcat
    40861 atttggatca aatagaccat ggttttcaaa aactgattta tgtatgacta ctgacgaagg
    40921 agatatcata cactgctcta gggattcatt aaaaattgag ccggattttt ttttatgttt
    40981 aagcttaggg ttaacccttt tgccgtaacg gtaccatatc tcttgcgatt gaaaaacatg
    41041 gtattgtggg ttttcctgaa taaattggta atcattttca agtttgttag taagccattc
    41101 atcgtcagag tccagaaatg cggtataagg tgttattaaa tctttaacag ctgtatttcg
    41161 tgcggacgat actccactgt tttctgtacg tattactgtt atgcggctgt ctttatattt
    41221 ggaaagaata ctggaagttt tgtctgttga gccatcgtct actataacaa atttaaaatc
    41281 cgaaaaggtc tgattgatta cagagtctac ggctcgacga atcgtttttt ctcggttaaa
    41341 taccgggatt attactgtaa atagtgaagt catatatagt ctttatccga tattgttaat
    41401 atgaaaccta atggcaacct caaaaaatta acttttcaac aaggaacttg cgaaaaattc
    41461 ctttaggata ggc
//
LOCUS       DAOTGZ010000005        36435 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_3064
            NODE_1257_length_36435_cov_5.397636, whole genome shotgun sequence.
ACCESSION   DAOTGZ010000005 DAOTGZ010000000
VERSION     DAOTGZ010000005.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868088
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 36435)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 36435)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:22:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,785
            CDSs (total)                      :: 2,751
            Genes (coding)                    :: 2,732
            CDSs (with protein)               :: 2,732
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 12 of 19
            Pseudo Genes (internal stop)      :: 5 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..36435
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1257_length_36435_cov_5.397636"
                     /isolate="AMR_MDS_3064"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..427
                     /locus_tag="P5123_00815"
     CDS             <1..427
                     /locus_tag="P5123_00815"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="PAS domain-containing protein"
                     /protein_id="HRX14833.1"
                     /translation="LFDVHVSATTVYDKEGNPVALTSASIEVTEQKNAERLLSESEER
                     FNLAMEVKNEGIWDWHIVSNDVIFDNRYYTMAGYDPGEFDQKLCSWTERVHPDDLSIC
                     NSAIEEYFIGKTECFDAEFRFKRKDGSWMWIQGRGKIVE"
     gene            545..1117
                     /locus_tag="P5123_00820"
     CDS             545..1117
                     /locus_tag="P5123_00820"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: protein motif:HMM:NF024970.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HRX14834.1"
                     /translation="MHHRVKNNIANIANLLSLQVGSTSNMEVKTALQDAASRVQSMRI
                     LYDRLLVSDDLREIPVKNYVDTLIDSIIQVFFDRKGISIKRTITDFNIAAKQAVIIGI
                     IINELLTNVFKYAFKDREHGNVLVLLKKNKNVVNLTIEDDGMGIESKENVNNSQGFGL
                     TIVKMLVEQLNGTKSIENRNGTRTEIRFEI"
     gene            complement(1158..3035)
                     /locus_tag="P5123_00825"
     CDS             complement(1158..3035)
                     /locus_tag="P5123_00825"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HRX14835.1"
                     /translation="MNSELLTALIPSTLLVFCSGILFVGTILSLYVYLTSKEMLYLST
                     AILGLLGTSLTAFEASVIILGIMQYPVAAFSFHRLQALTVTLFIPAMLFFVFQITNIS
                     VKQKKIFFVLLSTLLFSLLCIVIAFWHPDYFLLIKNHPSQYTALWNIGRGIAGPLYKI
                     RDVIIAFIALLSIICLAIESFSRHKKRHIQLAFTGAIIAIITGIADMAVASRELANGG
                     LFSIRVFSFFSIGLSCFIILGMIGVMKFFADRSREVEKARKLESLGILAGGIAHDFNN
                     TLATIVGNISLLRYTEPDPEQIEKSFFEIENAAYKARKLTNQLLTFSKGGGPVKAVTD
                     TKSILMDTVSFHLSGSAIKVAYDIPENLWNLNADSGQITQVIQNIVINAKQAMRSSGT
                     LTIRCVNIPSFTRPDNGKIIDEAVKIAFHNSGPIIPKKDLKKIFNPYFTTKSDGNGLG
                     LSICYSIIEKHNGYIGVTSEHNRGTEFIIYLPASKMKKTDNLPEKTTKLYDNKTALIL
                     EDDIGVQIVLSQMCKHINIEAIITSTGEEAIEKLKNCIISKTIPDIFIFDLTIIGGMG
                     GVETAKEVHTLVNDPKIIISSGYSNDPVIENYKNYGFCDYMQKPYRMQDFKDKILSVI
                     I"
     gene            3271..4653
                     /locus_tag="P5123_00830"
     CDS             3271..4653
                     /locus_tag="P5123_00830"
                     /inference="COORDINATES: protein motif:HMM:NF039907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TldD/PmbA family protein"
                     /protein_id="HRX14836.1"
                     /translation="MIDEKSISSLKGLVDSAGTDYAEIRISEIDKTIFSLRGDSVETV
                     SSGISLAGSVRIFDRGNWTFLSFNSIEEVQNHLLRAKELSMKLPAQKKELKKQSKPVS
                     IRKKSSPVIDFAEITPEQKFALCNEYNNILKGSPRIQTTRTMYRDAKSRDIYVNSEGS
                     DMYYEKLHCGVSLSAIAKEGNEIQTYQESFAGYGGFELVNNRHAEAEKVIAVALELLE
                     AQPIEGGKYSVITDPRLTGVFIHEAFGHLSEADFVYENNRMKKLMTLGKVFGPQFLNV
                     VDDGSIEDAVGYIPLDDEGVEPRKNYLIKNGELCGRLHSRETAGLMNEELTGNARAIS
                     AMAQPIVRMTNTYIENGKNTKDELFEEIGDGVYAVNAFGGQTNLEMFTFSSAYGRIIK
                     DGKPGKMVKNVMLSGNVFDTLKNVRMAANDMQLFGGLGGCGKGGQGPLPVSFGGPHLL
                     IDNVLIGGNQ"
     gene            4650..5942
                     /locus_tag="P5123_00835"
     CDS             4650..5942
                     /locus_tag="P5123_00835"
                     /inference="COORDINATES: protein motif:HMM:NF039907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TldD/PmbA family protein"
                     /protein_id="HRX14837.1"
                     /translation="MKGLNVDFLNSKCRWYDIIEVESRSTPVQFKNNKLYSIRESSSS
                     GLGLRVNSGGKTGITYTNDTKRVDQLAERAVALAAYGDKEDFDLPCGDTEYQRPVRPQ
                     KKEFCVDSEIEKGKAVIATIEEKYPDAEINMSITSGNSSSVLRNSNGLVYSDDSFSYS
                     ASCSVSLVLDDGTRIETGTSKVAYNPEPLNGFSEEIVSIIDMSLKTAKLDSGRIPMIL
                     TPRALRSILSILMGGLSAEQIYRKVSPYENKIGNSIFNKAFTLFDDPLMKDSPYSYGC
                     DDEGVASEKRALIENGTVNGFIADLKFASLLGVLPTGHSSRSYASKPGASFSNIEIAA
                     GDIPYENIIKECSTAIVVNQFLGLGQSNTYKGDFSANVDLGFLVKNGEIVGRLKDCMV
                     SGNAFDLFAGDIRVSSDVKEIPGARLPYMLFDGVNFTG"
     gene            complement(5961..6797)
                     /gene="purU"
                     /locus_tag="P5123_00840"
     CDS             complement(5961..6797)
                     /gene="purU"
                     /locus_tag="P5123_00840"
                     /EC_number="3.5.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018616576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008864 - formyltetrahydrofolate
                     deformylase activity [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formyltetrahydrofolate deformylase"
                     /protein_id="HRX14838.1"
                     /translation="MNIHRLLIDCTDNVGLVNKITGVLASHGVNILENDEFVEKDQNH
                     FFMRTEFTGLFDSGKLYSDLSTQLPENSIINLTGKRKKNIIVMVTKEHHCLGDVLIRH
                     AFGDLDANILAIISNHQNLRELSERMNIPFHYISHENLSREEHEKMVIKQVNQYNPEY
                     IVLAKYMRILTPAFTSLYPNRIINIHHSFLPAFIGANPYKQAFKRGVKIIGATAHFVN
                     HELDEGPIIAQDVVSVNHTNSAEEMARKGRDVEKIVLSRALGLVFEDRVMVNGNKTIV
                     FE"
     gene            complement(6794..7837)
                     /locus_tag="P5123_00845"
     CDS             complement(6794..7837)
                     /locus_tag="P5123_00845"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRX14839.1"
                     /translation="MKHLILITILIASMFYITSCSTLLPKKTTEEPTAIEPDSEEPET
                     EPENEFEKFELLNISKGEHADKIVLSWEADPEVTEEIIYHIYRADKFDSPYKKIAEAS
                     GSTWEDINTTKGLKLYYRIVPVVKTAELSTEEDTELTTVISTPEKMDTDHSNFGYARS
                     SYPKGTTFNALTRTYNKGRPKMSAAERKKANAEMEKLKERVMHSVKLRMMLAMVTPHM
                     NSGRVVALTDFSQIVVDKKNRLIYLFDKDYTYAVVLYSSHVTRNISSRTNKALYTRLA
                     LNSVSFSVKSDEIEVTDDKGFTRILPSFKSLALATQYFPKTRNWKNETMIFPTRDKEL
                     QREIQKAQGSPTR"
     gene            complement(8006..8758)
                     /locus_tag="P5123_00850"
     CDS             complement(8006..8758)
                     /locus_tag="P5123_00850"
                     /inference="COORDINATES: protein motif:HMM:NF027875.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycogen-binding domain-containing protein"
                     /protein_id="HRX14840.1"
                     /translation="MRFYILLSILFLHTIMLSAPDTVHEYSFKTLTKAQAPYEIKIIP
                     HIVRNKSTETGTLFTYDSRGSKSVLIAGDFSQWTPRPMKKGTNGIWYYFLSEYTSEAA
                     LRYKFLVDGIWISDPENPEAIDDGSGSFLSIAYPASSPEDKTVTYRFIRKNGIDYVEF
                     RTYNREASYISIAGDFNNWNPENNPLVKDENNIWRTKINLPEGKYRYKFIIDGNWSPD
                     IYNSDTAYDGMGGIASLIVVSPVTKRDIIILN"
     gene            9059..12394
                     /locus_tag="P5123_00855"
     CDS             9059..12394
                     /locus_tag="P5123_00855"
                     /inference="COORDINATES: protein motif:HMM:NF024824.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HRX14841.1"
                     /translation="MAVIKEKPEPVTYSAEELAEIERIVGTLPGSTEKSSDGTLMSLN
                     DEPPEELSSQPDQADYSAYNNDDLEDLALEPDSFIEEDGFEVEELNPEDSSENISLDA
                     DDLSALPELDDSSISFDIGEQEPSNIEPDMPFDLEDEISNDDISALPDEDISLDNFND
                     IGADLSSDEGLDELGAGFDLSDADDDAISDLDDGSMMDQLNDLTADEGDSIDLDDLAD
                     DEFLSASSGLSSGDSDDSVTIDGDDEMSLPDLNSLDMDDSSSIEEGDLTELPDVGFDD
                     LDSDSVENDASEVDDFSDQDIDIGLDDLSDDIGELPDIDHTDRSNADDISNLDDMDDI
                     GAMPIDEDIESSSILDDLEQIGTGDSGMSSGLDIDMPEIEPVDDHIATEGSSASKSGD
                     SLELSADELNKIKKSLLLYPAGLTAAVKDTILNDRLSPRDTRRVVDMILEGRPDHNIH
                     RFLEKKLGKAIDLEHGGVKRRVILSRPEYSSEGRARQKILLRRTRNIGLGMVLIAVLS
                     AVGYRTLYIPYRANSLIAKGTELIRRHNEPKFPDYKEAEQIFQRVHDDYISNYIRAYN
                     EYGRAYFDRKQYMESLLKLNRGYELVSDNFTGKDSIQLLNNLGYFYSSNKGVKFDDFF
                     NKSIKKNLDEWYFKKLSRLDVVNNQYDLAIDFYKKALSRDSKNMQALLGIGDVYMNQG
                     EFLKARAYYENILKIDPDSVAGHSGLFNLFVERDNFPEAISVYVNLRNKGMLENVPSP
                     LLAKGANYFLGKAKSDKQNIRIDYGIESPRLKDINDQPFPVVVEILKALRNKDPDYPP
                     LYVAYARLSIKEGNFRLAKEYLENGMRRSEKAGYSYFAGNHLLGEYYYYTRQPALSFK
                     HFKQALVDYSNPPEYAFEPYYKETENPGNTNAMLGNIFYYFFDRVKYQTGDQEMMTEM
                     DLRKNEELMGNFSIARSYYERALEMGFESPELHYNLGRVYYLNGEYSRTRDQWLNLYD
                     EFTVSPELMLGLGNVFYKMGNYEAAKAQFMKLASIYEYEAESIYQVVPDNREHKKIFQ
                     TLATAYNNLGAVYQQQNILDQSAICYWNSIELSRKIGEESAFARVNLGRGVKEREMPA
                     EPIFDDNIPYSISYYTEEMRWSR"
     gene            13124..13858
                     /locus_tag="P5123_00860"
     CDS             13124..13858
                     /locus_tag="P5123_00860"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRX14842.1"
                     /translation="MNAIIKITGFAMILLGSVSIAFAETLGSSNDLPFNENQEEVNVS
                     IKQNYASQKKHGKKTETLFGSEEFSVTGFGGPIYSMSNVEGKNYHFVGGRGGAVFNNT
                     FVLGGAGYGLTYPYKRQDFSDSAYQGSEDEVEMGYGGLLIGFNMFQKSIINVSFLTVV
                     GAGGLVVTDSRDDESDADSDYNTSNSDEFFVCEPTLMMHMNITKWMRIGAGVSYRYTR
                     GIDLAEFSDSDFSNISYVVAAEFGWF"
     gene            13997..15052
                     /locus_tag="P5123_00865"
     CDS             13997..15052
                     /locus_tag="P5123_00865"
                     /inference="COORDINATES: protein motif:HMM:NF013125.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lamin tail domain-containing protein"
                     /protein_id="HRX14843.1"
                     /translation="MKRGLLVVLSVIMLVISCEFDPANFDVTVGKDSDASITDIIIPD
                     KQYTIDFNGDDIRLIVDIVEEELSELRTETVFKGETLKVDGKIASNGISVCDYSNSTI
                     NPVVFIITAEDGTVRNFNVTISAVDSDIGDNQDGSSNDVIKPGLRDNCAGLMPGTNLR
                     ITEVIFHGDSVDDEYLEIYNPSGEDINLNRSSSPQDEFRIYRLTRSGNLNHVFSFPKD
                     SYIASGQTLRIANSNSIKYVESDFLFDDKKGLIASNEGVLLLTVFTCNSGIIVDLVCY
                     GISTDWNMWPEVIVDTNGPSIYSSVEEAVGNAGSPVLLNIYWPSNPVAIETGVYGIRR
                     TDKSQDTDTESDWGVIQ"
     gene            15049..16254
                     /locus_tag="P5123_00870"
     CDS             15049..16254
                     /locus_tag="P5123_00870"
                     /inference="COORDINATES: protein motif:HMM:NF019509.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospholipase D-like domain-containing protein"
                     /protein_id="HRX14844.1"
                     /translation="MKFVRILLLWAVFSSCAIPEPWVPEKTSVGIVCEVSFDPYVSEQ
                     LVVELIKNSEKTVEMALYGFSNEKIADELVNASKRGVVVRCVTDYDSQNEEGWKILFD
                     AIKRGERGLDVRLVNLNGIMHNKYIIVDGKSVVTGSTNFTSGMFKHFNNLVVFDSQSM
                     ALDFVKDFDVLYAGFSGPDKGCSLELNSDNNGFNRLYGDGIRWSEKNHVVDDLNVAVY
                     FTPYRAIFPSYLADSPRTFSFFDYDIGTESCASYANAMNTVFALIELAKHNIYILSFA
                     FTDKVMMDLLVDATERGVEVNVCSDYSQFRSSYSHSGKSVEALAEKINSFKLCRKDDG
                     GLLHHKVILIDGDIVVLGSLNFSSNAVNSNDENFLIIKNAESLYGLFLAEAGRVEKYC
                     REIEAADAL"
     gene            16244..17992
                     /locus_tag="P5123_00875"
     CDS             16244..17992
                     /locus_tag="P5123_00875"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRX14845.1"
                     /translation="MLYKHCIMAILLIVYACEVPEGKSDISIEKGDQTFIQKLEKMDN
                     PIGILPLRFKRDYTSSIVYQNENDYYSYFVFDETNALMILSDNPLYYICFPTKDSYFH
                     LLSEYRQLSKKTEPSKKNPNSIPEEYDYGNRRLKLSFIPNEARYSSRGGELIDINKDN
                     NDPEKLISIASKVGMFVPDFKEERIYNILAKRLRKLSVELTQVAAKVSFPPDFDASYL
                     TYHIEPNIPLGFAFFSGEKLIVDEEESDKIAAEDFIMFCSVPLGNSQKISESYLSKTE
                     SGRNGFIITIRGDIEEFKSFLSSEGFPFDNAGELPVSLVLEDKFDEGLYLECSSKESM
                     CYTISVNKGPDVPVKPDKIGDGRYYFKNIIRGNEYCVRAWRSERKIELLAESIIYCPK
                     IIISELMFWGSSYRKEDGANDDDWIEIVNISTDPVNLSEYDFIIINTALTQNFWFGPS
                     NHGTESHNALNFVLNPGERCVVALMVDDKDGVSGEFYFDQGCYPLDSKIVKKGTLSKN
                     SIGKGYQISIYNNEKLVTNVVTSDKYGSKLPFTSMVRLADGEFATSKNDSGYPAGVVY
                     EKRNFCSPGYAAPGEM"
     gene            17940..19388
                     /locus_tag="P5123_00880"
     CDS             17940..19388
                     /locus_tag="P5123_00880"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRX14846.1"
                     /translation="MKNETFVHRGMLLLVRCSLFLIIISFNISVFSQSNASLIVYSQN
                     AAFFADNMDYTSYSEDDYEKYRIDQNDNSDDRSIFYLSKIGIGADYATDRFNCVVRLD
                     KDYLWGGFDKYNSSNSGLSVNTLYAKVTLLPFVFLKTGRFYSSPGGALYDYVFGDTMD
                     GVRLDVVSPLGKIPLFLNLEAEVYAQNGKPAGIYGNLKDDESMPLNDFNGDTSSYRCG
                     IVTGVEPVRVIGNFLRYGASRDSGAQIAENGRSPLNKCDEDYLILTGLRIGTAEKTKV
                     LSWDITGLYSQGRDSRYSDAIMYKGYAVLAAVNMGNKQNDGLNHLFFHTGFYSPWFCG
                     MRSNTPGGVATDQMKGYVYAPVAGYNGFIDNSKRAGDGQGYDLTAPKAYLKTGGRVSL
                     SSFSFRMSYISIFVNESEDIWMRKNPLFTYIGSEGEFELVYSPGVFALSMGISLFKPS
                     EYYEKPNDDLSASGTDILYAFYTKLDYSFRLL"
     gene            19517..20734
                     /locus_tag="P5123_00885"
     CDS             19517..20734
                     /locus_tag="P5123_00885"
                     /inference="COORDINATES: protein motif:HMM:NF019310.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HRX14847.1"
                     /translation="MNYLSFFRKNPGVLVIGFMLTFFSSFGQTFLISLFVPDIMKELA
                     MSKSTFGSVYGAATILSSMTLAYVGKYADTMDIRKYTAATLLLLAISSAVTAVSVNIV
                     LVFVGFWGLRLAGQGLMSHISSTSISKIFNSERGSALSITSLGFAAGEMVFPVIAGIL
                     ILYSGWRFSMFVNAITALLLIPVIYIFMTKVAFHKTGESNIRKVRSQEFSRKDLFGDL
                     NFYIIALCSSVIPFVVTGLFFHQGALAQFKEWPMTLLATCMTGYASARAVFSIVGGRL
                     IDRFSARVLFPVFLMIFGLALLTVTFFACPVAAAIYLFLTGTAIGISGPVKTAVLAEY
                     YGTENIGGIRSLFSTLMVLCTAVSPALFGLILDNDLPFTYITGGSFILVTFCIGAGYR
                     LSIASESFLGSEA"
     gene            complement(20795..21379)
                     /locus_tag="P5123_00890"
     CDS             complement(20795..21379)
                     /locus_tag="P5123_00890"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRX14848.1"
                     /translation="MHLKRFFFLILLFLLNGCFSDFDYNFLDLSDDDTTLPVPTHSIY
                     INLSNNSSYWFEALLTISSINVINDSINRNTDYDNSILKPDESSEIGLIHFDSDYNDE
                     ELNNCIRFFNLILEFADGSKVYIEGLPEDLSDKNDAIYYGLGYHQGVNSGLVSSLDSD
                     NQYSAITYTITVNDKDDIEIHLNEDNSKVYSPED"
     gene            21677..22414
                     /locus_tag="P5123_00895"
     CDS             21677..22414
                     /locus_tag="P5123_00895"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRX14849.1"
                     /translation="MRSGWKFVIMSVLVLTIFGCTSVNIQKAEVSRVKTVALVGFEMY
                     RQTDGSTFGIVGDLVSSKKLSKNEKDLAESCYKVLTAQLGKEQKWKFVSIDKIRNNKG
                     YGKFFKENGRASKLILNIGKRYKIDGIATPKAISDMSYEERQNLIKELGVDAIASFDA
                     FIYQGESLSIAGFEYSKYRITLDSFEVYSNTSKDPIVSIKDLIGDTPETGHFAANINN
                     LVKLKTNTDKGILGATSEIAKSISEEM"
     gene            22811..23236
                     /locus_tag="P5123_00900"
     CDS             22811..23236
                     /locus_tag="P5123_00900"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRX14850.1"
                     /translation="MGRSIFLALVLSLFGCAQLHNVHITEFEAINTEGEPFEIIVDEA
                     GFSSDEAMSLVQESHKKSNFGQSSESVESLRAILALSQFGPKTGNPVFYTDYLDEIEK
                     KILEKCPSGKVVNLVSIRETRKSIIVSSEVVRIHGVCLK"
     gene            23233..23568
                     /locus_tag="P5123_00905"
     CDS             23233..23568
                     /locus_tag="P5123_00905"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRX14851.1"
                     /translation="MKKCMMMLLLMFFMLSLTMCTTLHSVSLSQIPKNRNQNIYAEAS
                     DWAFLGINFSNDIVEEAQSNLRAKCPNGKITGILTKFADYNYLFVVKREVTLNAFCVS
                     AEQTKGDME"
     gene            23565..23897
                     /locus_tag="P5123_00910"
     CDS             23565..23897
                     /locus_tag="P5123_00910"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRX14852.1"
                     /translation="MRHAILLVTIILILFATACTHAVHSYNVGNYRDTNGRIVSLAQS
                     EPISVKKSRHVILGFVFDTDYVKEAYDELLSRCENGEITGIQVRHITDLSFLNYTDHI
                     QITARCVR"
     gene            complement(24077..24592)
                     /locus_tag="P5123_00915"
     CDS             complement(24077..24592)
                     /locus_tag="P5123_00915"
                     /inference="COORDINATES: protein motif:HMM:NF040334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6144 family protein"
                     /protein_id="HRX14853.1"
                     /translation="MANKATRMLSNMSGQEKEQIIIKVGHIDETATPLKQARYINNFL
                     CAAEEMGVKTKNCMRKCGSDCISNNAIKIAKKLYSKSANISEFLEKLNEADIGGRNLH
                     LDNGHIIGIYKKCYCNIPKKVDVINQNYCECSAGWFEALFSTVFDKKVKVTIIDTITN
                     GAPECTFRIAY"
     gene            complement(24680..25558)
                     /locus_tag="P5123_00920"
     CDS             complement(24680..25558)
                     /locus_tag="P5123_00920"
                     /inference="COORDINATES: protein motif:HMM:NF018184.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HRX14854.1"
                     /translation="MNWIERMSKAMLFIDENIIEDIDIETIAATAQTTPAHFQRMFSI
                     ITGITPGDYIRHRRLTLAAQEINSTEISIIDAALKYGYETHSSFTKAFTKMHGFAPLY
                     ARKSGQSIKAYPPISFTLSIKGDTSMDYKIRDLKKTRLIGKTVNVSSANGENFKTIPT
                     FWENLAKQNMVEKIISFADRKGQMKGSLIGVCYDFDIDNESFKYMVGVESSSTDLPEG
                     FAEAELTHIKYAVFEANGPQPEAIQKTWKRIFSEWFPATKYIKANAAEMEVYLDKEPA
                     CECCEIWIPIETDTNA"
     gene            complement(25644..26816)
                     /locus_tag="P5123_00925"
     CDS             complement(25644..26816)
                     /locus_tag="P5123_00925"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRX14855.1"
                     /translation="MILKNSLFTNIFFLLLLLPLSYEEIHFAQETYVQPKPEQQQQKH
                     KPLDRKRSFKKFQTEPQEDSSIIDFYIKSSFELYHFAVLDDENNEKSTHDYTGRINKM
                     NYSFSPVYLASFNLYAAWSIFQFEGIYKTNRIIKGGGTIEKGDDAVSTITNDSQISEA
                     LGFGIQVFNLHTSFRTVHFNFGKADVVNAINDNTVQSQDLLLKVNEADIRYNSSYRLA
                     AFGPTSVVRYRFFLGYRYMQYSLPRIVYTFRKAEDTDQTDKKYIYKDETNPQEIPIKL
                     HMAGYGFETSVQERGSNLEIMSDLAFFYGAGNTKFKTRNSGELKERFLSSIQFQVKPG
                     LSYKLSKGMFKTSFKVQYEFNLLVPNPVNFSKNSNLYTFGQADLYHGLFVGFDLLF"
     gene            complement(26820..27224)
                     /locus_tag="P5123_00930"
     CDS             complement(26820..27224)
                     /locus_tag="P5123_00930"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRX14856.1"
                     /translation="MKRKTTLLLLILVLVATVILNCSRQKEDALIGCFKVEAGLKFKI
                     DKNTDQYFFTILDKDNPSESEKMYVPPREDLQALFEKDTETIKSYLVGENDSFGIFQI
                     NDTDKFMGEKPKSEFFVLMGFLQMYAEKTECE"
     gene            27457..28176
                     /locus_tag="P5123_00935"
     CDS             27457..28176
                     /locus_tag="P5123_00935"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRX14857.1"
                     /translation="MVKKSSIKKSKVENLESRGYVDDNFDYTRPYDILELEMMLKSVN
                     AVKRTQAALVIGYNRLSEMVPNLIISLQKETKLYSKIAICNALYVIGDASIDPLLDNL
                     GLIGNNQYNELPDKAFGKGGYPLPRDIVARTLVRMGEKALIKTVNSSDNKPRGWLLEA
                     VDVMGHISFYNQSTIASGKLLELYYENINDLLLRWKLIRAFSAFELDEILAILQKEVE
                     ESRVPAIKWEAERSLRMIGDE"
     gene            28178..29110
                     /locus_tag="P5123_00940"
     CDS             28178..29110
                     /locus_tag="P5123_00940"
                     /inference="COORDINATES: protein motif:HMM:NF015722.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SUMF1/EgtB/PvdO family nonheme iron enzyme"
                     /protein_id="HRX14858.1"
                     /translation="MSEHKSCFKSSDLLMTIKIAMFGILAILAIILFVVKINRQYDLN
                     KNTDIFVYIEGGSFVQQDTDAVSGKVYASFDHTISSFSIAKYETTYQLWHDVYRWAKK
                     SGYFFANPGKEGAWGMPGHLAVRYLGEPVTSVSFYDVVVWCNAYSQMKKLMPVYNNEN
                     GEPIRDSRDSNMDELAKMVVNRSANGFRLPTEGEHRFAATCRGKYGNPAVNQNMSYSP
                     KPVALTDANELGLCGMAGNVWEWCWDWDGSLPKNAQLDYHGPDTGTGKIIAGGRAFYS
                     GMLFAGNRVGIMPDYYNGYIGFRLVKNLTDRDLK"
     gene            29674..30270
                     /locus_tag="P5123_00945"
     CDS             29674..30270
                     /locus_tag="P5123_00945"
                     /inference="COORDINATES: protein motif:HMM:NF024242.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HRX14859.1"
                     /translation="MITVTGSLAWIVNGIRFFSFGTVYSHVAGLFFTIIIFGTVLICF
                     YFFLNYPSVILGNRDIQGRTQKSPSEKYLKTRMAIHEVERIMSLVEKVMKDEKLLESE
                     EISLNSIACKVGCEPYILSQAINRKTGGNFYNYVNSYRIAYSKEFLRSPEHVSMTVLE
                     IAYRSGFSSKSSFNEAFKKHTGVTPTQFRNRSLKHCEI"
     gene            30367..31233
                     /locus_tag="P5123_00950"
     CDS             30367..31233
                     /locus_tag="P5123_00950"
                     /inference="COORDINATES: protein motif:HMM:NF015722.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SUMF1/EgtB/PvdO family nonheme iron enzyme"
                     /protein_id="HRX14860.1"
                     /translation="MRKVKKCFIVLFVLLSTLSLFPKSGIKNTFPLSDMIFVKGGSFI
                     QQDANAVSGKVNKSFIHNVSSFYIGKYEVSYEIWKSVYDWAVIKGYTFDNVGSPGMVE
                     EVKIKDTSKLPITNISYYDALVWCNAYSEKFQYEPVYYSADGEVVRDIRNLKREEKKN
                     IYANWEANGYRLPTEGEWYFAARSRGAEGNIKIKQKMTDRIYDVDDISCNKLGLYGVD
                     HNVWEWIWDWYGTLPSGKKRDYRGPNSGDGKITLGGQAYICDHIISGHRLGVITSIKN
                     SFIGLRVARSGQ"
     gene            31340..32008
                     /locus_tag="P5123_00955"
     CDS             31340..32008
                     /locus_tag="P5123_00955"
                     /inference="COORDINATES: protein motif:HMM:NF012257.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0030552 - cAMP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HRX14861.1"
                     /translation="MNPLELLQQNELFGELDFTFINNIAKNAHYHHATKGELLFIEGE
                     PGDSFYLIADGRVRLFKSAGNDRETTIKTALPGEIIGEVILFESGVYPVSAQAVTDSK
                     LLFLHRSEFMGFLEDSAFRNAFLAVLMRKQRYLAERIKVLSSLDVEERFLLYLSKLPA
                     ENDAIKITENKSVIAGEIGTIPETFSRMIKKMKQRSVLEWKGDLVKIDKNYMDSVLEE
                     ILIS"
     gene            32016..32735
                     /locus_tag="P5123_00960"
     CDS             32016..32735
                     /locus_tag="P5123_00960"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRX14862.1"
                     /translation="MSSGITEESRLEDNRIIRYLRDENVFTEERVRHAYRMLSKQTHP
                     DITGKDSSDFIRLREEYERVLEGIDSLARYFSVEVSASPAYDEKYCRLMFYLSLKRYM
                     TVGLYSVKLRIRESVRQRNRIILEDVVKRAQDYDPSFIPLFLNYNRNLLRRFSLWYSS
                     SGRRNKNGRNYFIKGINFFLDYELRGSREMFNITTSYLNDAVYELGDGDNDPVNTAMY
                     GISCWLLRELKRKSLFDRSAI"
     gene            32814..33455
                     /locus_tag="P5123_00965"
     CDS             32814..33455
                     /locus_tag="P5123_00965"
                     /inference="COORDINATES: protein motif:HMM:NF012876.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0098561 - methyl accepting chemotaxis
                     protein complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein"
                     /protein_id="HRX14863.1"
                     /translation="MKKPRTTIRKRMIFYFLLIAIANIFVAGEIIYEMKSEDYRNIVV
                     QKMYQVQQGVLEPEVIFEIIDQWIRKFSVMISILIAVSAVILFLFVVQIASPLHYMIK
                     QAVKMSEGDLSGMIKVNTNDELAEMAMLVNDMSQNLQEVLAQLERISEDVQYTVQLHF
                     RNINRHSKLPAVFKKETLNLHKIQQDMILLKSCFKLYRVSFKPDKKSGTDNRD"
     gene            33541..34104
                     /locus_tag="P5123_00970"
     CDS             33541..34104
                     /locus_tag="P5123_00970"
                     /inference="COORDINATES: protein motif:HMM:NF020136.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CIA30 family protein"
                     /protein_id="HRX14864.1"
                     /translation="MKKFVFCVVFLSLIIFPVFNVGGQALESPDKWFTYNDKGDGGDS
                     IIKLSKGKQTIGGKEYLVISCTGEVTKKFEYGFIGFGIRLEDENLKRFQNAKGIKFKV
                     IGDGKSYRVRTESSAVTDYDYHGKVFSAKGSVTEISVPYSSVKQEGWGAPKGSFKKNT
                     LVQISFQTVGQPHESVSLKVFDLQPMN"
     gene            34548..35531
                     /locus_tag="P5123_00975"
     CDS             34548..35531
                     /locus_tag="P5123_00975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004510689.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidylglycerol lysyltransferase
                     domain-containing protein"
                     /protein_id="HRX14865.1"
                     /translation="MGTLHIGEYELMPFDISAKAVMDKYLAQLDLDTSDYTFAANYLW
                     LSNGTGFYSIINETFCFFLLTSGELSMLLPPVGKKANVIDAMLVCFQLMDDNNSSPFY
                     SKIEYVDEILVSDFINNLEDGAEIFDMLEEYIIERKLVDYLYDANDLIELRGGSYSSK
                     RNEINKFMRINPDHVLDTLDVEKHGAGILNLVNKWIADRMKYMPREEADAFLDGIYSE
                     RIAVKRMLKDYKALDLTGLVIIINGEIKGFTVGEKVNSNTASVIIEKTDFEIFGCAQY
                     IFREFVRILANDYNVTSINVGDDMGFENLKKVKLSYRPSKLLPKYTIYKRY"
     gene            35531..35989
                     /locus_tag="P5123_00980"
     CDS             35531..35989
                     /locus_tag="P5123_00980"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024917.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HRX14866.1"
                     /translation="MMQVSIRSADKSDIKELVEIEKSSFVPSDGPLSSKSFRYHIERS
                     NILFIASVSINDKYVAAGYALVLPRKNSMRLYSIAVNPVYRGKGVARELLKEVIQCAA
                     KRKVASVYLEVRPDNVAAILLYKKFGFCKTAELSDFYGEGSHGIRMIAYL"
     gene            complement(35986..>36435)
                     /locus_tag="P5123_00985"
     CDS             complement(35986..>36435)
                     /locus_tag="P5123_00985"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HRX14867.1"
                     /translation="AKESVIYNIKNLNTSKEYDITKALFCVISPETDTQLTIKNHLNR
                     NGYHNIIQIEDSSKAFDVIKKNNPDIIICELISTDLCRQINSDPETESTPLLLLTRIK
                     EKRLLRSLSENGISALLLKPVNETELIGLAKYHIKNNFLIRKLLNSN"
BASE COUNT        11076 a         5687 c         7828 g        11844 t
ORIGIN      
        1 tctttttgat gtacatgtat cggctacaac ggtctatgac aaggaaggca accctgtggc
       61 gttgacatcc gcctccatag aggtgacgga gcagaaaaat gccgagaggt tgttaagcga
      121 aagtgaagaa agatttaatt tggccatgga ggtcaagaac gagggtattt gggattggca
      181 tatcgttagt aatgacgtta tttttgataa ccgatactac acaatggccg gttatgaccc
      241 gggtgaattt gaccaaaagt tatgttcatg gactgagcgt gttcatcctg atgatttatc
      301 gatttgtaat tctgcaattg aagaatattt tattggcaaa accgaatgtt ttgatgcgga
      361 gtttcgtttt aaacgtaaag acggatcatg gatgtggata cagggacgag gtaaaatagt
      421 tgagtgagat gaaaatgggg agccattaag aatcgtaggc actcatacag atattacgaa
      481 acgcaaacag gtagaggaag agaaccaaaa acagcttgcc gagaaagagc tgctgttgcg
      541 tgaagtgcat catcgcgtta aaaacaatat tgccaatata gcaaatctat tatcattaca
      601 ggttggctct acctcgaata tggaagtcaa gactgcgtta caggatgccg cttctcgtgt
      661 gcaaagcatg cgtatactat atgacaggct tttggtatcg gacgatcttc gcgaaatacc
      721 ggtaaaaaat tatgtggata cccttataga ttctataata caggttttct ttgatcggaa
      781 aggtataagc attaaaagaa caattactga ttttaatatt gctgcaaaac aggcggtaat
      841 tatcggtatc attattaatg agctgttaac aaatgtcttt aaatatgcgt ttaaagatag
      901 agagcatggt aatgttcttg ttttgctgaa gaaaaataaa aatgtggtta acttaactat
      961 cgaggatgac ggtatgggaa ttgagtctaa agaaaatgtt aataacagtc aaggatttgg
     1021 tttaaccatt gtcaaaatgc ttgtggagca gttaaacgga accaaaagta ttgaaaatag
     1081 gaatggaacc agaactgaaa ttcggtttga aatataatta ggataaagtt ccgaatagta
     1141 actgaaaagc agattgctta aatgataaca gaaagaattt tatctttgaa gtcttgcatt
     1201 ctataaggtt tttgcatata atcacaaaat ccataatttt tatagttttc gattacggga
     1261 tcgtttgaat agccgctgga aataataatt ttggggtcat taacaagagt gtgtacttct
     1321 ttagcggttt cgactcctcc catcccgccg attattgtaa ggtcaaagat aaaaatatcg
     1381 ggtatggttt tgctaattat acaattttta agtttttcga tggcttcttc accagtagag
     1441 gtaattatcg cttcaatatt aatatgttta cacatctgtg ataaaacaat ttgtacgccg
     1501 atatcgtctt ctaaaataag agctgtttta ttatcgtata atttggttgt tttttctggc
     1561 aggttatctg tttttttcat tttacttgca ggcaagtaaa taataaattc tgtgccacga
     1621 ttatgttcag aagtaacgcc aatatatccg ttatgctttt cgatgattga atagcaaatg
     1681 gataaaccaa gaccgttgcc gtcacttttt gttgtaaagt agggattgaa tatttttttt
     1741 aaatcttttt ttgggataat tggtccactg ttatgaaaag ctatttttac agcctcatcg
     1801 attattttgc cgttatcggg tcttgtaaaa cttggaatat ttacacagcg tatcgttaag
     1861 gttcctgaac ttcgcattgc ctgcttggca ttgatgacga tgttttgtat tacctgtgtt
     1921 atttgaccag agtcggcgtt taagttccat aaattttcag ggatatcata ggcaaccttt
     1981 attgcgctac cggatagatg aaacgaaacg gtatccatca agatgctttt tgtgtcggtt
     2041 actgctttaa ccggacctcc tcctttggaa aaagtgagta attggtttgt aagttttcga
     2101 gccttgtaag cggcgttttc aatctcaaaa aacgactttt ctatctgttc tgggtcaggt
     2161 tctgtatatc gaagcaatga tatattgcct actatagtag ccagtgtatt gttgaagtcg
     2221 tgggcaattc caccagccaa gattcctaaa gattcaagct ttctggcctt ttcaacttct
     2281 cgtgaacggt cagcaaaaaa tttcataacg ccgatcatgc caagaattat gaagcatgac
     2341 aaaccaatac tgaaaaatga aaaaactctt attgaaaata gtccaccgtt agccagctct
     2401 cttgatgcaa cagccatatc ggctattccg gtaatgattg cgattatggc gccagtaaag
     2461 gcaagctgga tatgcctttt tttgtgtctg gaaaaagatt cgattgccag acaaataatg
     2521 gaaagtaacg caataaatgc tattataaca tccctgattt tatatagagg accggcaatg
     2581 ccacggccta tgttccataa agctgtatat tgtgatggat ggtttttgat aagtaaaaaa
     2641 tagtcgggat gccaaaacgc tataacaata cataatagag aaaaaagtag agtggacagt
     2701 aaaacaaaaa atattttttt ttgttttaca gaaatgtttg taatttgaaa cacaaaaaaa
     2761 agcatagccg gtatgaatag tgtaacggtt aaagcctgga ggcggtgaaa agaaaaggcc
     2821 gctacaggat attgcattat tcccagaatg attacagagg cctcaaacgc agtaagagat
     2881 gtgcccagaa gccctaatat tgctgttgat aggtagagca tctcttttga ggttaggtaa
     2941 acgtacaggg aaagaattgt tcctacaaaa agtattcctg agcaaaaaac aagcaatgtt
     3001 gatggtatta aagctgttaa tagttcactg ttcattcctg gatcctgtgt attatcatgt
     3061 aaataaatat atggttacat agtataatgt caaacagtaa tttatgcata aagaactttg
     3121 atataagttt cttattttga gtttaaaatt atcagaatat atgaaagcgg ttttgtacgt
     3181 atgtttatac gatatcatga attggttgac agttaatttg gttataaagt aaatttatag
     3241 aaataacatt aaaaccgatc ggaggattga gtgatagacg agaagagtat aagctcactt
     3301 aaagggctgg ttgacagtgc cggcactgat tacgcggaga ttcgtatttc cgagatagac
     3361 aaaacgattt tctctcttag aggtgatagt gtggagaccg tttcgtcggg aatttcttta
     3421 gcgggttcgg ttcgaatatt tgaccgggga aactggacct ttttgtcgtt taattccata
     3481 gaagaagtac aaaatcattt attaagagcg aaagagctgt ctatgaaatt gccagcccaa
     3541 aaaaaagagc taaaaaaaca gtcgaaacct gttagcataa ggaaaaaaag ttctcctgtt
     3601 atagattttg ccgagattac ccctgaacaa aaatttgccc tttgtaacga atacaacaat
     3661 atactaaaag gttcccctcg catacagact acacgtacca tgtaccgtga tgcaaaatcc
     3721 agagatattt acgttaatag tgaaggctct gatatgtatt atgaaaaatt acattgcggg
     3781 gtctctcttt cggcaatagc taaagagggt aacgagatac agacctacca agagtctttt
     3841 gccggttatg gagggtttga gcttgttaat aacagacatg ctgaggccga gaaggtaatt
     3901 gcagttgcgt tagaactttt ggaagcacag ccgattgaag gtggcaagta ttctgtaata
     3961 accgacccgc gccttaccgg agtatttata catgaagcat ttggacatct ttcagaagcc
     4021 gattttgtat atgagaacaa ccgaatgaag aagctgatga cgctgggtaa ggtttttggg
     4081 ccacaatttt taaatgttgt tgatgatggt tcaattgaag atgcagtcgg ttatatcccc
     4141 ctggatgatg agggagtcga accgcgtaaa aattatttaa taaaaaacgg tgaactatgt
     4201 ggtcgtcttc actctcgtga aaccgcgggc ctaatgaatg aagagctaac aggtaatgcc
     4261 agagcaattt cagccatggc acaacctatt gtacgaatga ccaataccta tattgaaaat
     4321 ggaaaaaata caaaagacga gctttttgaa gagatcggtg acggagttta tgctgttaat
     4381 gctttcggag gacagacaaa tctggagatg tttaccttct cttcggcata cggtcgaatt
     4441 atcaaagacg gaaaaccggg aaaaatggtg aaaaatgtga tgctctcggg taatgttttt
     4501 gatacgctga aaaatgttag aatggcagcg aatgacatgc aactatttgg aggattggga
     4561 ggctgtggaa aaggtggaca aggaccgttg cctgtatcgt ttggcgggcc acatctgcta
     4621 attgacaatg tacttatcgg aggcaaccag tgaaaggatt gaacgttgat tttttgaatt
     4681 caaaatgccg ctggtatgat ataattgagg ttgaaagccg gtctactcct gtacagttta
     4741 aaaacaataa actttacagt ataagggaaa gttccagcag tggtttggga cttcgtgtaa
     4801 acagtggcgg caaaacagga attacttata caaatgatac aaagcgtgtg gatcagcttg
     4861 cggagcgcgc tgttgcgtta gccgcatatg gagataagga agattttgat ctcccatgtg
     4921 gtgatacaga atatcagaga ccagtacgac cccaaaagaa agagttttgt gttgatagtg
     4981 aaattgaaaa aggaaaagct gtaatcgcaa cgattgaaga gaaatatcca gacgcggaga
     5041 ttaatatgag tattacctcc ggtaatagta gttccgtttt acgaaactca aacgggcttg
     5101 tttatagtga tgattcgttc tcttattcag cttcttgttc tgtttcgctt gttttggatg
     5161 acggaaccag gatcgagaca ggaacaagca aggttgctta caatccggag cctctaaacg
     5221 gtttttcgga agagatagta tccataattg atatgagttt aaagacagca aaattggaca
     5281 gcgggcgaat accaatgatt ttgacaccaa gagctcttcg ctcaatattg agtattctta
     5341 tgggaggttt atcagctgaa cagatatatc gtaaggtttc tccctatgag aataagatag
     5401 gcaactctat ttttaataag gcgttcactc tttttgatga tcctttgatg aaggattctc
     5461 cttatagtta cggttgcgat gacgaaggtg tagcttctga aaagagagct cttattgaga
     5521 acggtacagt aaatggtttt attgccgatc tgaaatttgc ctcgcttctt ggggtgttgc
     5581 ctactggtca ttcttcacga agctatgcgt ctaaaccggg agcatctttt tccaatatcg
     5641 aaatagcggc aggcgatatt ccgtacgaaa atataataaa agagtgttca accgctatag
     5701 tggttaatca gtttctgggt ttggggcaat ctaatacata taaaggtgat tttagtgcaa
     5761 atgttgatct gggttttttg gtaaaaaatg gagagattgt tgggcgatta aaagattgta
     5821 tggtaagtgg taatgctttt gatctttttg ctggtgatat ccgggtatca tcggatgtaa
     5881 aagagatacc cggggcaagg ctaccctaca tgttgtttga tggggtaaat tttactggct
     5941 gattcatgta aaagtgccgt ttattcaaaa acaatagttt tatttccgtt aaccataact
     6001 cggtcttcaa aaacaagccc caaggctctt gaaaggacaa ttttttcgac atcacgtcct
     6061 ttgcgagcca tttcttccgc agaatttgtg tggtttacgg agacaacatc ttgggcaatg
     6121 ataggccctt cgtccagttc atgatttaca aaatgtgctg ttgctccaat aattttaaca
     6181 ccacgtttaa atgcctgctt ataggggttt gctccaataa atgccggtaa aaaagagtga
     6241 tgaatattta tgatccgatt tggatagaga gaggtaaacg ccggtgttaa aattctcata
     6301 tatttggcta gtacgatata ttccggatta tactggttta cctgcttgat taccatcttt
     6361 tcgtgttctt cgcgactgag gttttcgtga gaaatgtaat gaaagggaat gttcattctt
     6421 tcagacaact ctcgtaaatt ttgatgattg ctgattatag cgagaatgtt ggcgtcaagg
     6481 tcaccaaaag catgtcttat tagaacatct cccaagcagt gatgctcttt ggtgaccata
     6541 acgataatgt ttttcttgcg ctttcctgtc aggttaatga ttgagttttc cggcaattgg
     6601 gtactcaaat cagagtaaag tttaccggaa tcaaaaagac cggtaaactc ggttctcata
     6661 aaaaaatggt tttgatcctt ctcgacaaat tcatcattct cgagaatatt gaccccatga
     6721 ctggccaaaa cccctgttat tttgttaaca agcccaacat tgtcggtaca gtcaattaat
     6781 agccggtgaa tattcatctt gttggacttc cctgtgcttt ttgaatttct ctttgaagct
     6841 ccttgtccct tgttggaaaa atcatagttt cgtttttcca gtttcgggtt tttggaaaat
     6901 attgtgttgc aagggctaaa cttttaaatg agggtaaaat tcttgtaaaa cccttgtcat
     6961 cagtaacttc aatttcgtca cttttaacgc tgaacgagac gctgttaaga gcaaggcgag
     7021 tgtaaagagc tttatttgtt ctggatgaga tattgcgtgt tacatgagag ctgtataaga
     7081 ccacagcata agtgtagtct ttgtcgaata gataaattaa acggtttttt ttgtcgacaa
     7141 ctatctgaga aaaatctgta agtgctacaa cacgacccga gttcatatga ggagtaacca
     7201 ttgccagcat cattctaagt ttaacagaat gcatgactct ttcctttagt ttttccattt
     7261 ctgcgttagc ttttttgcgc tcagcggcac tcatcttcgg ccgccccttg ttataggttc
     7321 gtgtaagtgc gttaaaggtt gtaccttttg gataagatga acgggcatag ccaaaattac
     7381 tgtggtcggt atccattttt tcaggtgtag aaataacagt tgttaattca gtgtcttctt
     7441 cggtagacag ttcagcagtt tttactacag gaacaattct ataataaagc ttaagacctt
     7501 ttgtcgtgtt gatgtcttcc caggttgaac cggaagcttc tgcaattttt ttatatggag
     7561 aatcaaattt gtcggctctg taaatatgat aaataatttc ttcagttact tcgggatcgg
     7621 cttcccagct aagtacaatt ttgtcggcat gttcgccctt tgatatattt agcaattcaa
     7681 atttctcgaa ctcgttttca ggttcagttt caggttcttc tgaatctggt tctatagctg
     7741 ttggttcttc agttgttttt ttgggtagca aagttgaaca tgacgtaata tagaacattg
     7801 atgcaatcaa tatcgtgatt agaataagat gtttcataaa gcctccagtt ttataattgt
     7861 gatgcgacta tttctatagt gaatatgtgc tacaaatcaa gtcttttgtt aaaaaaaata
     7921 tggcatatca gataatttga gtttgataat gtataaattt agtattaaat taaaatgata
     7981 tcaaaatcgg tagactttgc caaagttaat ttagaatgat aatgtcacgc tttgttacag
     8041 gtgaaaccac aataagagag gcaatgccgc ccatgccgtc atacgcagta tcgctattat
     8101 agatatcagg agaccaattg ccatcaatta taaatttgta tctatatttc ccttcgggaa
     8161 gattgatttt tgtacgccaa atattgtttt cgtcttttac aagcgggtta ttctcaggat
     8221 tccagttgtt aaaatcgcct gcgatcgaaa tatatgatgc ttcacgatta taggttctaa
     8281 attctacata gtcgataccg ttttttctta tgaaacggta tgttacggtc ttgtcttctg
     8341 gtgaagaagc cggatatgct attgataaaa aagatccact accatcatca attgcctccg
     8401 gattttccgg atctgatatc catataccgt ctacaagaaa cttgtatcgc aaagcagctt
     8461 cagaggtgta ctcggataaa aaatagtacc agattccgtt tgtacctttt ttcataggcc
     8521 ttggtgtcca ttgactgaag tctccggcaa ttaaaacaga tttggagcca cggctgtcgt
     8581 aagtaaaaag agtcccagtt tcggtacttt tgttgcgtac aatatgcgga atgattttaa
     8641 tctcataggg tgcctgggct tttgtcaggg ttttaaaaga gtactcgtga actgtgtccg
     8701 gggccgatag cataatagta tgcagaaaaa gtatactaag taggatgtaa aacctcattg
     8761 agacctcttt taagcatgtt aatatcaaat tttaagttga tctacttata ttttcgtaga
     8821 aacaggtaaa actattgatt aaaaaaagag ttttgttgat ttaaagcgaa ttataaagaa
     8881 ttaagtttca tttagtagtc aggtaaagtt accgataatt aaaaaggtat tgatttttat
     8941 agtatggata ttatatctga ttatgtcgct tgtaattctc catatggaac gaaaattggt
     9001 ttattatcaa taaaacggat gattatgctg atgtacaaaa tagtagttcg ggtagttaat
     9061 ggcggtaatt aaagaaaagc cggagccggt aacctactcc gccgaggagt tggctgaaat
     9121 tgaaagaata gtcggaaccc ttcccggtag caccgagaag agctctgacg gtacacttat
     9181 gtctttaaat gatgagcccc ctgaggaatt atcttctcaa ccggatcagg cagattattc
     9241 tgcttacaat aatgatgatt tggaagatct tgctttagag cctgattcat ttattgagga
     9301 agatggattc gaagttgaag agttgaatcc ggaagattcc tcagaaaata tcagtctgga
     9361 tgcggatgat ttatccgctt tacccgaact tgatgacagc tcgatatcat ttgacattgg
     9421 cgagcaggaa ccctccaata ttgaacctga catgcctttt gatctggaag atgaaattag
     9481 caatgatgat atttcagctt taccggatga agatatttct ttggataatt ttaatgatat
     9541 tggtgctgat ctatcttcgg atgaaggatt ggatgagtta ggtgccggat ttgatctttc
     9601 tgatgctgac gatgatgcta tctctgacct agatgatggg tcaatgatgg atcagcttaa
     9661 tgatttaact gcggacgagg gagactccat agatttggat gacttggctg atgatgaatt
     9721 tttatcagcg tcatctggtt tgtcttccgg agacagtgat gactctgtta cgattgatgg
     9781 tgatgatgag atgtcactcc ctgatctcaa ttctcttgat atggatgatt cttcttctat
     9841 tgaagaaggt gatctcacgg agctaccgga tgtcggtttt gatgatttgg actctgattc
     9901 ggtagaaaat gatgctagtg aggttgatga tttttcagat caggacattg atattggtct
     9961 tgatgatttg agtgatgata ttggtgaatt gccagatatt gaccatacgg acagaagtaa
    10021 tgccgatgat atctcaaatc ttgatgatat ggatgatatc ggagccatgc cgatagatga
    10081 ggatattgaa tcaagttcga ttttggatga tcttgaacag attggaacag gtgattccgg
    10141 tatgtcttct gggctggata ttgatatgcc agaaattgaa cctgttgatg atcatatcgc
    10201 aacagaagga tcctctgctt caaagagtgg agactctctt gaattaagtg ctgatgaact
    10261 taacaagata aaaaaatcac ttctacttta ccctgccggt ttaaccgcag cggtaaaaga
    10321 tacaattctt aatgatcgtt tgtcgccaag agatacgcgc cgtgttgtag atatgattct
    10381 tgagggacgc cctgatcata atattcacag gtttcttgag aagaaactgg gcaaggctat
    10441 cgatttagaa catggaggag tgaaaagacg agttattctc tctcgacctg agtatagcag
    10501 tgaggggcgg gcacgccaga agattctctt gcgccgaacc cggaatattg ggctagggat
    10561 ggttttaatt gccgtgttgt cagctgtggg ttatagaaca ctttatattc cttacagggc
    10621 aaattccctt attgcaaaag gaaccgagtt aataagacgt cataatgagc ccaagtttcc
    10681 cgattacaag gaagcggagc agatattcca gcgtgttcac gatgattata tatccaatta
    10741 tataagggcg tataatgagt atggacgagc atattttgac cgtaagcagt acatggaatc
    10801 gctgcttaaa cttaatcgcg gttatgaact ggtttcggat aattttaccg gcaaagattc
    10861 aatacagctt ttaaataatc tgggatattt ctattcttcg aataaaggtg tcaagttcga
    10921 tgattttttc aataaatcga taaagaaaaa tcttgatgag tggtatttca aaaaattatc
    10981 gagattggac gttgttaata accagtacga cctggcaata gatttttata aaaaagcatt
    11041 gagccgtgat agcaaaaata tgcaggcctt attgggtatt ggtgatgttt atatgaacca
    11101 gggcgagttc ttaaaagctc gcgcttatta tgaaaatatt ttgaaaattg atcctgactc
    11161 tgttgccgga cattcgggtc tttttaatct ttttgtggaa agggataatt ttccggaagc
    11221 tatttccgtg tatgtgaatc tgcgcaacaa agggatgctt gaaaatgtgc catcaccgtt
    11281 attggcaaaa ggggcgaatt atttcttagg aaaagctaaa agtgataaac agaacatacg
    11341 tattgattat ggtatagaat ctccacgatt aaaagatatt aatgatcaac catttccggt
    11401 tgttgtagaa atcctcaagg cgctaaggaa caaggatccc gattatccgc cgctttatgt
    11461 agcatacgcg cgactttcga taaaagaggg aaactttagg cttgctaaag agtatctgga
    11521 aaacggtatg aggcgttctg aaaaggctgg atacagttat tttgccggaa atcatctttt
    11581 aggagaatat tactactata cacgtcaacc agctttgtca tttaaacatt ttaaacaggc
    11641 tttggtcgat tacagtaatc cgccagagta cgcgtttgaa ccgtattaca aagaaactga
    11701 aaatcctgga aatactaacg caatgctggg taatatattt tactatttct ttgacagggt
    11761 aaaatatcag acaggtgatc aggagatgat gaccgagatg gatctccgca aaaatgaaga
    11821 gctgatgggg aatttttcta tcgcaagaag ctattacgaa agagctttgg agatgggatt
    11881 cgaatcgcca gagttacatt ataatcttgg aagagtctat tatcttaatg gagagtattc
    11941 tcgaacacgt gatcagtggt taaatcttta tgacgaattt actgtttcac ccgagttaat
    12001 gcttgggctt ggaaacgtat tttacaaaat gggtaactac gaagcggcca aagctcagtt
    12061 tatgaagctt gcttccatct acgaatatga agccgaatca atttatcagg tagtgcctga
    12121 taatcgggaa cataaaaaga tctttcaaac attggcaacg gcttataata atcttggggc
    12181 agtttatcaa caacaaaata ttcttgatca gagtgctatt tgttactgga attcaataga
    12241 gctttcccgt aaaattggag aggagagcgc ttttgccaga gtaaatcttg gtcgtggagt
    12301 taaagaacgt gaaatgcccg cagagccaat ttttgatgac aatattccct acagtattag
    12361 ctattataca gaagagatgc gatggagtcg ctagtttcgt ttgcttaagg taagcattca
    12421 tcagtttgtc cttgattttt atcatggtaa taaatggaat agttcacaac tgtcgttcac
    12481 aactgtcgtt cacaactgtc aattttttct ttttaatatg actattaaac taatagttat
    12541 tggaattccg catttaatga tcaattgaga gcgaaagtaa taatttaaag ctatagctcc
    12601 atatgtatta agaatttcca ttacatgtaa tgaaaatatt atatttaata ggtatcccca
    12661 attgattata taaaaatatg aatcaataaa accaaaatca agatgaaata ttgctataat
    12721 tacatagctt agaaatatta tactcgatag aataaggcat atataggcaa gaatgtattt
    12781 tatgtttctt tttgttacta gtgccgagat tgccaataca agggttaata ccaacaccgt
    12841 attctccttt cgctctttgt agcgaacatc tgctgtttag ttgctacatt aaaaaataaa
    12901 actaacaatg taattgtcaa taccttaaaa tcagtttgtt aacaataata atcaagcata
    12961 acaaagagaa aactaaaaat agttattggt gcacattgca taaactcaca taaatttata
    13021 tcaaaaaaaa gattttttga agaatacggt gtcatttttt tcgagattcc tgttatatat
    13081 ataaacgtgg gtgaacaagt accctttatt atgtgaggta attatgaatg caataattaa
    13141 aataacaggt tttgcgatga ttcttttggg ctcggtatca attgcttttg cagagacttt
    13201 gggaagtagc aatgatttac cgtttaatga aaaccaggaa gaggttaatg tcagtattaa
    13261 acaaaactat gcttcccaaa aaaagcatgg caaaaaaaca gaaactcttt ttggctctga
    13321 ggagttttct gtaacaggtt ttggtggccc gatctattcg atgtcaaatg ttgaaggcaa
    13381 gaactatcac tttgtcggtg gtcgtggagg agcagtattt aacaacactt ttgttttggg
    13441 cggagccgga tacggactta cctatccata taagcgtcaa gatttttccg attctgccta
    13501 tcagggctcg gaagatgagg tggaaatggg atacggaggt cttctgattg gttttaacat
    13561 gtttcaaaaa agtataatca atgtgtcttt tctaactgtg gtcggtgcag gtgggctggt
    13621 tgtaacagat agtcgcgatg acgagagtga tgctgatagc gattataaca caagcaatag
    13681 cgatgaattt ttcgtttgtg agccaacttt gatgatgcat atgaatataa ccaaatggat
    13741 gcgtattggt gctggtgtat cttatcgtta tacacgcggt atagatcttg ctgaatttag
    13801 tgattcagat tttagtaata tatcctatgt ggttgccgct gaatttggat ggttttgaaa
    13861 aatttagtag atttgaatta gttaagtaat gtcaaaaccc gcgaaagcgg gtttttttgt
    13921 gtttttttaa aaaagttcgc cctttttttt gatttcatcg ttatagtata tgatttcaaa
    13981 aaaaggaggg tagaatatga aaagggggtt gttggttgtt ttatcagtaa tcatgttagt
    14041 aatatcatgt gaatttgatc cggcaaattt tgatgttaca gtcggaaaag attcggacgc
    14101 gtccataacc gatattatta taccagataa acagtacact attgatttca acggtgatga
    14161 tattaggtta atcgttgata tagttgaaga agagctttcc gaattgcgaa cagagactgt
    14221 ttttaaaggc gaaactctta aggttgacgg gaaaattgcg tcaaacggaa tatcagtctg
    14281 tgattattct aattctacta ttaatccagt tgtatttatt attaccgcag aagacggtac
    14341 tgtgagaaac tttaacgtga caatatcggc ggttgattcg gatattggtg ataaccagga
    14401 tggctcatca aacgatgtga taaaacccgg gcttcgtgat aactgtgccg ggttaatgcc
    14461 gggtacgaac cttcgcataa cagaagttat ttttcatgga gactcggttg atgatgaata
    14521 tttagagata tataaccctt caggcgagga tattaatctt aaccgtagca gttcacccca
    14581 ggacgagttc agaatatatc gtttaacaag atcgggaaac cttaaccatg ttttttcttt
    14641 ccctaaagac agctatattg cttcaggaca aacactcaga atagccaata gcaatagtat
    14701 caaatatgtc gaaagtgatt tcttatttga tgataaaaaa gggcttattg cttctaacga
    14761 aggggtattg cttttaacag tttttacctg taatagtgga attattgttg atctggtttg
    14821 ctatggaata tcgacagatt ggaacatgtg gccggaagtt attgtcgata ctaatgggcc
    14881 atcgatatat tcgtcggtag aagaagcggt tggaaatgct ggaagcccag ttcttcttaa
    14941 tatttattgg ccatcaaatc cggttgctat agaaaccggc gtatatggaa taaggcgaac
    15001 tgataaatcc caggatacag atactgaatc tgattgggga gtaattcagt gaaatttgta
    15061 agaatactat tgctttgggc tgtttttagc agctgcgcga ttccagagcc atgggtgccc
    15121 gaaaaaacat ctgtcggtat tgtgtgtgaa gtatcttttg atccgtatgt tagcgaacag
    15181 ttggttgtcg aactaattaa aaattcagaa aaaacagtag aaatggcgtt atacggtttt
    15241 tcaaatgaaa aaatagcaga tgaacttgta aatgcttcta aaagaggcgt tgttgtgcgg
    15301 tgtgtaaccg attatgattc acagaacgaa gagggttgga aaattttatt tgatgctata
    15361 aaacgaggag agagggggct tgatgtaaga ttggtgaatc taaatggaat aatgcataac
    15421 aagtatatta ttgtagacgg taaaagtgtg gttacaggtt ctaccaattt tacatcgggt
    15481 atgtttaagc atttcaataa tcttgttgta tttgatagtc aaagcatggc attggacttt
    15541 gttaaagatt ttgatgtctt gtacgctgga ttttctggtc cggacaaagg atgctctctt
    15601 gaactgaatt ctgacaataa tggatttaat agattatatg gagatggaat ccgatggagc
    15661 gaaaagaatc atgttgtaga tgatttaaat gtggctgtat attttactcc ttaccgtgcc
    15721 atatttcctt cgtatttggc agattcgccg cgtacatttt ctttctttga ttatgatatc
    15781 ggtactgaaa gttgtgcatc atatgcgaat gcaatgaata ctgtgttcgc attgattgaa
    15841 ttggcaaaac ataacattta tattcttagt tttgcattta ccgacaaggt aatgatggac
    15901 ctgcttgtgg atgcaactga gcgtggtgtt gaagttaatg tgtgtagtga ttattcacag
    15961 tttagatcta gctattcgca ttctggaaag agtgtcgaag ctcttgccga aaagataaat
    16021 tcatttaaac tatgtcgtaa ggatgatgga ggcctattgc accataaagt aatattaatt
    16081 gatggcgata ttgtagtttt gggttcgtta aattttagca gtaacgcagt aaattcaaat
    16141 gatgaaaatt ttctaattat aaaaaatgcg gaatcgttat atgggctctt tttggccgaa
    16201 gctggccggg tagaaaagta ttgccgtgaa atcgaggccg cggatgcttt ataaacactg
    16261 tattatggct attcttctga ttgtttatgc atgtgaggtt ccagaaggta aaagtgacat
    16321 tagtatagaa aaaggagatc agacctttat ccagaagctt gaaaaaatgg acaatccaat
    16381 tggtattctt ccgttaagat tcaaacggga ctatacttcg agtatcgtct atcagaacga
    16441 aaatgattat tattcatatt ttgtttttga tgaaaccaat gccttaatga ttttatcaga
    16501 taatccgctc tattatatct gttttcctac taaagacagt tattttcatc ttctttccga
    16561 gtatagacaa ctttctaaaa aaacagagcc aagtaaaaaa aatccgaaca gtattccgga
    16621 agaatatgat tatggtaaca gacgcttaaa gctctctttt attcctaatg aagcaagata
    16681 ctctagcagg ggtggtgaac ttattgatat taacaaagat aataatgatc cggaaaaact
    16741 gatatctatt gcttcgaaag taggaatgtt tgtcccggat ttcaaggaag aacgtattta
    16801 caatattctt gcgaaacgtt taagaaaatt atctgtagaa ttaacgcaag tcgccgcgaa
    16861 ggtttctttc ccgccagatt ttgatgcctc atatttaacg tatcatatag aaccaaatat
    16921 tcctcttgga tttgcatttt tttcgggaga gaagctgata gttgatgaag aagaatcaga
    16981 caagatagct gctgaagatt ttattatgtt ttgttcagta cctcttggca attcacagaa
    17041 gataagtgaa tcgtatctta gtaaaacaga aagtggtcgt aacggattta taataaccat
    17101 acgcggtgat atagaagagt ttaaatcctt tctatcaagt gaaggttttc cttttgataa
    17161 cgctggtgag ttgccggttt cacttgttct tgaggataaa tttgatgaag gtctatatct
    17221 cgaatgtagc agtaaggaaa gcatgtgcta tacaatctct gtaaacaaag gtccagatgt
    17281 tcctgttaaa ccggacaaaa tcggcgatgg gcggtattat ttcaaaaata taattcgagg
    17341 aaatgaatat tgtgttaggg catggcgttc tgaaagaaag atcgagcttt tagcagaatc
    17401 gataatatat tgtccgaaga taataattag tgaacttatg ttttgggggt cttcttatcg
    17461 caaagaagat ggagcaaacg atgatgactg gattgaaata gtaaatataa gcactgatcc
    17521 ggttaatctt tctgaatatg atttcattat aataaataca gctcttactc aaaatttctg
    17581 gtttgggcct tctaatcatg gaacagaatc acataacgct ttaaatttcg ttctaaatcc
    17641 aggtgaacga tgtgttgttg ctcttatggt tgatgataaa gatggtgttt caggtgaatt
    17701 ttattttgac cagggttgtt atcctcttga ttcaaaaatt gtgaagaaag gaactctatc
    17761 caaaaattca atcggaaagg gatatcaaat ttcaatatat aacaatgaaa aacttgttac
    17821 aaatgttgtt acgagtgata aatacggctc caaacttccg ttcacatcaa tggttcgact
    17881 tgctgacggt gagttcgcaa cttcaaaaaa tgattctggt tatccggcag gggttgtcta
    17941 tgaaaaacga aacttttgtt caccggggta tgctgctcct ggtgagatgt agtctgtttt
    18001 taataataat ttcttttaat atttcggtgt tctctcaatc aaatgcttca ctgattgttt
    18061 attcgcaaaa cgcggctttt tttgcagata atatggatta tacgagttat agcgaagatg
    18121 actatgagaa gtatcgaatt gaccaaaatg ataacagtga tgacagatcg atattttatt
    18181 taagcaaaat cggtattgga gcagattacg caactgatag gtttaattgt gtagttcgtc
    18241 ttgataaaga ttatttatgg ggtggatttg ataagtacaa cagcagtaat tctggattat
    18301 ccgtaaatac attatatgca aaagtaacac ttttgccgtt tgtttttttg aaaacgggaa
    18361 gattttacag ctctcctggt ggagcactct atgattatgt gttcggagac accatggatg
    18421 gtgttagact ggatgtagtt tcgccgttag ggaagatccc gctgtttctg aatctggaag
    18481 cggaagtata tgcgcaaaac ggcaagcctg caggaatata tgggaatttg aaggatgacg
    18541 aatcgatgcc gttaaatgat tttaatggtg atacttcaag ttatcgttgt ggtattgtta
    18601 ctggagttga accggtcagg gtgataggta actttcttag gtatggagca tcgcgagaca
    18661 gtggtgcgca gatagcggaa aatgggaggt cacctttaaa taagtgcgat gaagattacc
    18721 tgattttgac gggtttacgg attggtactg ccgagaaaac aaaagtatta tcatgggata
    18781 ttaccgggct ttattcgcaa ggtagagata gtcgttattc ggacgctatt atgtataaag
    18841 gctatgcagt gctggctgca gttaatatgg gtaataagca gaatgatggg cttaatcatc
    18901 ttttttttca cactggtttt tatagcccct ggttttgtgg tatgcgttct aatacacccg
    18961 gaggagtagc tactgatcag atgaaaggtt acgtttatgc gccagttgct gggtataacg
    19021 gctttatcga taatagcaaa cgagcgggtg atggccaggg gtatgatctg actgctccta
    19081 aggcatatct aaaaactggt gggcgagtca gtttatcatc tttttcgttt agaatgagtt
    19141 atatctcaat ttttgttaat gagagtgaag acatctggat gcgaaaaaat ccgttgttta
    19201 catatattgg atccgagggt gaatttgaac tggtctattc tccaggagtg tttgccctga
    19261 gtatgggtat ttcgttattt aaaccatctg agtattatga aaagcctaac gatgatttgt
    19321 cggcatcggg aacagatatt ttgtacgcat tttatacaaa actggattat tctttcaggc
    19381 ttttgtaggc cttttatgtg atttttaaag aaaaatgttg aacaaatggt tgttgttgaa
    19441 gaaatgtcca tgtcaagatt ttgttaattg ccggatcaaa gtggttcatg gcgctgatat
    19501 attaaggatt gattagatga actatttatc tttttttaga aaaaatcccg gggtgcttgt
    19561 tatcgggttt atgcttactt ttttttcaag ttttggtcaa acatttctta tatctctttt
    19621 tgttcctgac atcatgaaag agcttgcaat gagcaagtcg acttttggtt ctgtttatgg
    19681 agccgcaaca atattaagtt ctatgactct cgcttatgtc ggtaaatatg ctgacaccat
    19741 ggacatacgc aaatatacgg ccgctactct tcttctgctg gcgatctctt ctgctgttac
    19801 cgctgtatct gtaaatattg tgttagtttt tgtcggtttt tggggattgc gcttagcagg
    19861 tcaagggctg atgtctcata tttcgagtac atccatatca aagattttca attcagaacg
    19921 gggtagcgcg ctcagtataa cttcactggg attcgcagct ggtgagatgg tttttccggt
    19981 tattgctggg attcttatcc tctattcggg gtggcgtttt tccatgtttg ttaatgcgat
    20041 aaccgctctg ttgctgatac cagttatata tatattcatg actaaagtag cttttcataa
    20101 aaccggtgag tcaaatatca gaaaggtgcg atcacaagaa ttttcgcgaa aagatctttt
    20161 tggagacctt aatttctaca ttatagcctt atgctcttca gtcattcctt ttgttgttac
    20221 gggacttttt tttcatcagg gcgcccttgc ccagttcaag gagtggccta tgactttgct
    20281 tgcaacttgt atgactggtt atgcgtcggc aagagcagtt ttttctattg taggaggaag
    20341 actcatcgat agattttcag caagggtgct tttcccagtg tttctgatga tttttggtct
    20401 tgcactttta accgtgactt tcttcgcttg cccagtggcc gctgctatat atcttttttt
    20461 aacaggtact gcaatcggaa ttagcggccc agtcaagaca gctgttttgg ccgaatatta
    20521 tggaaccgaa aacatcggag gcatcagatc tcttttctca acattgatgg ttctctgtac
    20581 agcagttagt ccagctcttt ttggtttgat actggataat gatttgccgt ttacatatat
    20641 tacaggtgga tcatttatac ttgttacatt ctgtattggt gcaggatacc gtttaagtat
    20701 tgctagtgaa tcatttttgg gtagtgaagc ttagagatta aaaaatagcg cttttttaat
    20761 ctcttttcga acataaacat taatttgtac cggtttaatc ttctggagaa taaactttgg
    20821 aattatcttc atttaaatga atttcaatat catctttatc gttgactgta attgtatatg
    20881 taattgcaga gtactgattg tcgctatcaa gtgagctaac taaaccgctg tttactcctt
    20941 gatgataacc taggccataa taaatagcat cgtttttatc tgatagatct tccggaagcc
    21001 cttcaatata aacttttgaa ccatctgcaa attctaaaat cagattaaaa aatcggatgc
    21061 agttgttcag ctcctcatcg ttataatctg agtcaaaatg tattaaacca atttcagagg
    21121 attcgtcagg tttaagaatg gagttatcat aatcggtgtt tctatttatt gaatcgttta
    21181 ttacattgat gctggaaatt gtaagtaatg cttcgaacca gtaagaagag ttgttggata
    21241 gattgatgta aatagagtgt gtgggtactg gtaatgtcgt atcatcatct gacaaatcta
    21301 aaaaattata atcaaaatct gaaaagcatc catttaataa aaataacagt attaagaaga
    21361 aaaatctttt taaatgcata attaactcct tgttgtttca caaaattgtg taagagcttg
    21421 caataatttt gagtatgcta ttaaatatta tttgtcaata gttttaattt ttatacagaa
    21481 aaattctaaa accgagtaaa atactacttt gcttgaatag cataattcaa ataaattgac
    21541 acttaactgg aaactgtttg ctgatagtta ttagaaatat taagagtaat aaatatcaga
    21601 gtaaacaaaa aaagcttgta actttaaaaa tataattata accaatataa aatatttatt
    21661 aaaattggag cctattatga gatctggatg gaaatttgtt attatgtctg ttttggtttt
    21721 aactattttt ggatgtacta gtgtaaatat tcaaaaagct gaagtatcaa gggtgaaaac
    21781 ggttgcgctg gttggttttg aaatgtacag acagacagat ggttcaacgt ttggtattgt
    21841 tggggatttg gtttcttcaa aaaaattgag taaaaatgag aaagatttgg ctgaaagctg
    21901 ttataaagta ttaactgctc aattgggtaa agaacagaaa tggaaatttg tttctattga
    21961 taaaattaga aacaataaag gttatggcaa attctttaaa gaaaacggaa gagcaagtaa
    22021 acttatcttg aatattggta aaaggtataa gatcgatggt attgccactc caaaggcaat
    22081 tagtgacatg tcttatgaag agagacaaaa tttgataaaa gagcttggag tagatgcaat
    22141 tgcaagtttt gatgctttta tatatcaggg cgagtcgctt agcattgctg gttttgaata
    22201 tagtaagtat cgcataactc ttgattcatt tgaggtttat agcaatacca gcaaggatcc
    22261 aatagtgtcc ataaaagatc ttataggcga tactccggaa acgggtcatt ttgctgctaa
    22321 cattaataat ctggttaagc ttaaaacaaa tactgataag ggtattctag gtgcgacatc
    22381 agaaatagcc aagagtattt ctgaagaaat gtagttgtta tataattaaa tatatatcgt
    22441 ttttaaaata gttggtatta caacatgtcc atggtatttt ttagattatc acaccatgga
    22501 catcgtattg tttataaaga taaattatct ccggtttaat gattggctaa aagccaatta
    22561 acgggcttcg gatcagaata tagaggtcct tgcagatgcc ccatccatgt atattcacta
    22621 atatttataa tctctatttt ttttgggcat ttgttatcag ataattgtta taaaaaacag
    22681 gggaaaagag gcatcgtata cggtttattt taagtgttaa tttaaaaaag caggtttagg
    22741 attgcataat tagatagaga taaattacat agatatagaa tatgaaaaat acactgcaag
    22801 gatggataat gtggggagat caattttttt agctctggtt ctgtcacttt ttggctgcgc
    22861 acagctacat aatgtgcata ttacagagtt tgaggccata aacaccgagg gcgaaccgtt
    22921 tgaaattatt gtagatgagg ccggtttttc aagtgatgaa gccatgagtc ttgttcagga
    22981 atcacataaa aagagtaatt ttggacagtc aagcgaatca gtagaatcat taagagcgat
    23041 tcttgcttta tcacagtttg gccctaaaac cggaaacccg gttttctata ccgattatct
    23101 ggatgaaatt gaaaagaaaa ttcttgaaaa atgtccaagc ggaaaggttg ttaatcttgt
    23161 aagtatccgt gagactcgta aaagcataat agtttcgagc gaggttgtta gaatccatgg
    23221 agtgtgtcta aaatgaaaaa atgtatgatg atgctactgc taatgttttt tatgttatct
    23281 ttgactatgt gtacaacctt gcatagtgtt tcgttgtcgc aaattcctaa aaatcgcaac
    23341 caaaatattt atgctgaagc tagtgactgg gcgtttttgg gaattaattt ttctaatgac
    23401 attgttgaag aggcgcaaag taatttaagg gcgaaatgcc ccaacggtaa gataacagga
    23461 atattaacaa agtttgctga ttataattat ctttttgttg ttaaaaggga agttacttta
    23521 aatgcttttt gcgtatctgc agagcagact aaaggtgata tggaatgaga catgctatat
    23581 tgcttgttac aattattttg attttgtttg cgacagcctg tacccacgcc gttcattctt
    23641 ataatgtcgg caattatagg gatacaaatg ggcgcatagt ctctcttgcc caaagtgagc
    23701 caatatctgt aaagaaaagc agacacgtaa ttcttgggtt tgtttttgac accgattatg
    23761 ttaaagaggc atatgatgaa cttctgagcc gttgtgaaaa cggagaaatt accggcatac
    23821 aggttcgcca tataaccgat ttaagctttc taaactatac agatcatatt cagattacag
    23881 caagatgtgt tcgctaggca accttagtca ggtaatatta agcataaatt taacagcagg
    23941 atgttttatt actatcctgc tgtctttgtg atcggctttc agtttaaact atttattgat
    24001 gttttaattc tgaaaaagct gaaagactgc ctgggtagga gccgtttaca aactgtggac
    24061 tgaggttgga taaatcttaa taggcaattc taaaggtgca ctctggggct ccgtttgtaa
    24121 tggtatcgat aattgttact tttacttttt tatcaaatac agttgaaaaa agtgcttcaa
    24181 accatcctgc agagcattca caatagtttt ggtttattac atcaactttt tttggaatat
    24241 tgcagtagca tttcttgtat attccaatta tatgtccatt gtctaaatgc agattgcgtc
    24301 ccccgatatc ggcttcgttt agtttctcaa gaaattcgct aatatttgcc gatttagaat
    24361 atagtttttt ggctattttg atggcgttgt ttgatatgca gtcacttccg catttccgca
    24421 tacagttttt tgttttaact cccatttctt cggcagcaca taaaaaatta tttatatatc
    24481 tggcctgttt aaggggtgtc gcagtttcat caatgtgacc tactttaatt ataatctgct
    24541 ctttttcttg tccgctcatg ttagataaca tacgtgttgc tttgttagcc atggttatct
    24601 cctgtaagat tatttatcat gatttgattt ggagggttat gttgaataac cctccggttt
    24661 acaaaattta ttgtaaatat caagcgttag tatcggtttc tattggaatc catatttcac
    24721 agcattcgca tgcgggctct ttatccaagt agacttccat ttcggcagca ttagctttga
    24781 tatattttgt tgcaggaaac cactccgaga aaatacgctt ccatgttttt tgtatagcct
    24841 cgggttgcgg gccgttagct tcaaaaaccg cgtattttat atgtgtaagt tcagcttcag
    24901 cgaaaccctc aggtaggtct gtcgagctac tttcgacacc gaccatatac ttaaagctct
    24961 cattatcaat atcgaaatca tagcagaccc cgataagaga acccttcatc tgtccttttc
    25021 ggtcggcaaa tgagataatt ttttcaacca tattttgttt ggccagattt tcccaaaaag
    25081 ttgggatagt cttgaagttt tcaccattag cggatgatac attaactgtt ttacctataa
    25141 gtcttgtttt ttttaaatct ctgatcttgt agtccatgct tgtgtctcct tttattgaaa
    25201 gtgtgaagga gatgggagga tatgccttga tgctttgccc gcttttgcgc gcgtaaagag
    25261 gggcaaaacc gtgcatcttg gtaaaggctt tggtaaacga tgagtgtgtt tcgtaaccat
    25321 actttaacgc ggcgtctatt attgagattt ccgtggaatt gatctcttgt gccgccaacg
    25381 taagacgacg gtggcggata tagtcgcccg gagtaatacc ggttataata gaaaacattc
    25441 gttgaaaatg tgccggtgtt gtttgtgcgg ttgcggcaat ggtttcgatg tcaatatctt
    25501 caatgatatt ttcgtctata aataacattg ctttactcat ccgttctatc cagttcatcc
    25561 ttttctcctt ttgattaata taaaagagcg ttgctggaat gtctttgcct taaatgttct
    25621 tggtggacaa acagtgcgtt tttttaaaaa agaagatcaa accccacaaa aagtccgtga
    25681 taaagatctg cctgaccaaa cgtgtacaaa tttgaatttt tagaaaagtt tacaggatta
    25741 ggaacgagaa gattaaattc gtactggact ttaaatgaag ttttaaacat accttttgat
    25801 agtttataag agagaccggg ttttacttga aactgaatag aagataaaaa tcgttctttt
    25861 aattcaccac tgtttctggt tttaaatttt gtatttcccg cgccgtaaaa aaaagcaaga
    25921 tcagacatga tttctagatt tgatcctctt tcttgtacac tcgtttcaaa tccataacca
    25981 gccatgtgga gcttgatagg tatctcctga gggtttgttt cgtctttata aatatatttt
    26041 ttatctgttt gatcagtatc ttcggctttg cgaaaggtat aaacaatccg cggaagactg
    26101 tattgcatat agcggtaacc gagaaaaaaa cggtaccgga caactgatgt tgggccaaat
    26161 gcagcgagac gatatgagct gttataacgg atatcagctt catttacttt taacaataga
    26221 tcttgtgatt ggacagtatt atcgttaatc gcgttaacaa catctgcttt accaaaatta
    26281 aagtgaaccg tgcgaaacga ggtatgaaga ttgaacacct gaattccaaa ccccagagct
    26341 tctgagattt gagaatcgtt ggttattgtg gaaacagcat cgtctccttt ttcgattgta
    26401 ccgccgcctt taatgatcct gttagtcttg taaattccct caaattggaa tatgctccat
    26461 gctgcgtata gattaaaaga tgccaggtat accggggaaa acgagtaatt catcttatta
    26521 attctgccag tgtaatcatg tgtggatttc tcgttgttct cgtcatcaag cacagcaaaa
    26581 tgatacagtt caaaagatga cttgatgtaa aaatcaataa tgctactgtc ttcttgaggt
    26641 tcggtttgaa attttttaaa acttcttttt cgatctaaag gcttatgttt ctgttgttgt
    26701 tgctcaggct ttggctgaac atatgtctcc tgagcgaaat gtatctcttc ataagaaagt
    26761 ggtagcaata ataacaaaaa aaatatattt gtaaataatg aattttttag gatcatatat
    26821 cactcacatt ccgttttttc tgcgtacatt tgtaaaaatc ccattaacac aaaaaattcc
    26881 gattttggtt tttcgcccat aaatttgtct gtgtcgttta tttgaaaaat tccaaaagaa
    26941 tcattttctc caacaagata agatttgata gtctctgtgt ctttttcaaa tagtgcctga
    27001 aggtcttcac gaggcggcac gtacattttt tctgattctg aaggattgtc tttgtctaaa
    27061 atagtaaaga agtactggtc tgtatttttg tctattttaa acttgagtcc ggcttctacc
    27121 ttaaaacagc cgataagcgc gtcctctttt tgtctgctgc aatttaatat tacagttgca
    27181 actaaaacca gaattagcag taataatgtt gtttttcttt tcataaattc tcctgttacc
    27241 tgcttggata aaaagcccga tagggttgaa aactaagaat ttattatttg tgtttggaaa
    27301 gcaactaaaa attgttaatt gtcatcagta tttttgagtt tacaggatta tctgaaaatt
    27361 ttatgtaata ggtaatagtt gggatatgaa aatttcgaag tatgttatat ataagctttg
    27421 gtgctgctat aataacaggg gatgataatc agaataatgg taaagaagag tagcattaaa
    27481 aaaagcaaag tggaaaacct tgaatctaga ggatatgtgg atgataattt tgattatacc
    27541 cgaccgtatg atattctaga gctggaaatg atgctaaaat cggtgaatgc tgttaagcga
    27601 acgcaggcag ctcttgtgat aggttataac cgcttatctg aaatggttcc gaatcttata
    27661 atatccttgc aaaaagagac gaagctttac agcaagattg caatttgtaa tgccctttat
    27721 gttataggtg atgccagtat agacccgctt cttgataatc tcggtcttat tggtaataat
    27781 cagtacaatg aacttccaga taaggctttc ggtaaaggtg gctatcctct tcctcgagat
    27841 attgttgctc gtactcttgt gcggatgggg gaaaaagcat tgataaaaac agtaaatagt
    27901 tctgataata agcctagggg ctggcttttg gaagccgtgg atgtaatggg acatatatcg
    27961 ttttataatc aaagtactat agcgtctgga aaacttcttg agctctatta cgaaaatata
    28021 aacgatctac ttttaagatg gaaactgatt cgtgcttttt cggcttttga attagacgag
    28081 attcttgcca tattgcaaaa agaagttgaa gaaagcagag tgcctgctat aaaatgggag
    28141 gccgaacgca gcctgagaat gattggagat gagtgatatg tcagaacata aatcttgttt
    28201 taaatcttca gatcttctta tgaccatcaa gattgccatg ttcggtattt tggcaattct
    28261 tgcaataatt ctctttgttg tgaaaattaa ccggcaatat gatttgaata aaaatactga
    28321 catatttgtc tatattgagg gtgggtcctt tgttcagcag gatacagacg ctgtatccgg
    28381 aaaggtttat gcgagtttcg atcatacgat ttcatctttc agtattgcaa agtatgaaac
    28441 gacatatcaa ctttggcatg atgtttatag atgggctaag aagagtggat atttttttgc
    28501 caatccaggt aaggaagggg cttggggaat gccagggcat cttgctgtaa gatatcttgg
    28561 tgaacctgtg acatctgttt cattctatga tgtggtagtc tggtgtaatg cttatagtca
    28621 gatgaagaaa ttaatgccgg tctataataa tgaaaacgga gaacctattc gtgactcgcg
    28681 ggattcaaat atggatgagc ttgcgaaaat ggttgtaaac aggagtgcta acggttttcg
    28741 gttgcctacc gagggcgaac acaggttcgc tgccacatgt cgtggcaaat atgggaatcc
    28801 tgctgttaat cagaatatgt cttattcacc gaagcctgta gccttgacag acgcaaacga
    28861 actggggttg tgcggtatgg cgggtaatgt ctgggagtgg tgttgggatt gggatgggtc
    28921 actcccgaaa aatgcacagc tggattatca tggacctgac actggtacgg gaaagattat
    28981 tgccggagga agggctttct attcgggaat gcttttcgcg ggtaaccggg ttggaatcat
    29041 gccggattat tataatggtt atatcggatt cagactggtt aaaaacttaa cagacaggga
    29101 tttaaaatga atcccgtaat ttttgaattc atgacattat tgattttggg agtactactt
    29161 gtctttgtct ttattaccta ttttgtaaaa aaactccgtt gccctatgga aaaccggtat
    29221 ttgtctatat tgctggtgtt atttggtttt agaactcttt tcgaacaatt tagtctggaa
    29281 ccctggtata gtcactatga ttataatgta tggcaatcca ttaatttcgt gttcgcgcct
    29341 ctgacctata tttatattaa aacattattt tgcaaatcgt ctattaagca tgttccggtt
    29401 ataaaatata aatttccaca ctttatattg gcagttgctg tttttataat tttattgcca
    29461 tttttaatta atagaatgat tgccaacagt aaattgcaga ctataaatgg gttttttgaa
    29521 atagaaaata tcgttaatat agttgttccc attttaaatt attttcaggt ttttgtctat
    29581 tatatcatga tgtcacatct gttgttaata aacaaaagat cttgcaatga tagcaataac
    29641 aagtcggaca tgataagata tcggtgggct ttaatgataa ccgtgacagg tagccttgct
    29701 tggatagtga acggtataag gtttttctct tttggaactg tttactctca cgttgccgga
    29761 ctcttcttta ctattataat tttcggtact gtgttaattt gtttttactt ttttctaaat
    29821 tatccgagtg ttattcttgg aaaccgtgat atacaaggca gaactcaaaa aagcccatct
    29881 gaaaagtatc ttaagacaag aatggctatt catgaagttg aacgtattat gagccttgtt
    29941 gaaaaggtta tgaaagatga aaagctgctg gaaagtgaag agatcagctt gaatagcatt
    30001 gcctgcaagg tggggtgtga gccttatatt ctatcccaag cgattaatcg aaaaactggc
    30061 ggaaattttt ataattatgt taacagttac cgtattgcgt attcaaaaga atttttgcga
    30121 agtccggagc atgtttcaat gaccgttcta gagatagcat acagatcagg gtttagctcg
    30181 aaatcatcat tcaatgaagc ttttaagaaa catacaggag tgactcctac tcaattcaga
    30241 aatagatctt tgaaacattg tgaaatatag aaaatatttc aaaaatttgt atggttttat
    30301 aaggagagac gatttattta ataaacagtg gtactgtttt tgcatgaagc aaaaggagga
    30361 cctaatatgc gaaaagttaa aaaatgtttt attgttttgt tcgtactgtt atcaacttta
    30421 tcactgtttc caaaatctgg aataaaaaat acgtttccat tatctgatat gatttttgtc
    30481 aagggtggga gttttattca gcaggatgcc aacgctgtta gcggaaaggt caataagagt
    30541 ttcattcata atgtctcttc attttatatt ggaaaatatg aagtatctta tgaaatatgg
    30601 aaaagtgttt atgactgggc tgtaattaaa gggtacactt ttgataatgt agggtcgccc
    30661 ggaatggtcg aagaagttaa aataaaagac accagtaaac ttccgataac aaatatcagc
    30721 tattatgacg cactggtctg gtgcaatgcc tatagcgaaa aatttcaata tgaacctgtc
    30781 tattattcag ctgacggtga agttgtacgc gatataagaa atctgaagcg tgaagaaaaa
    30841 aagaatattt acgctaattg ggaagctaac gggtatcggt tgccaacgga aggagagtgg
    30901 tatttcgcag ccaggagtag aggggcagag ggcaatataa agattaagca aaaaatgaca
    30961 gaccgtatat atgatgtaga tgatatatcg tgtaacaaat tggggctata cggtgtggat
    31021 cacaatgtat gggaatggat ctgggattgg tatgggacgt tgccatctgg taaaaaacga
    31081 gactatagag gtccaaatag cggtgatggc aaaataaccc tcggtgggca ggcctatata
    31141 tgtgatcata taatatctgg ccatcgtttg ggtgtaataa caagcattaa aaacagtttt
    31201 attggtttgc gtgtcgcaag gagtggtcag taaatctttg acttgtcaat gtggtgcata
    31261 attgaattat aaaacgattc attgttgtaa caatatgaaa agaattgact aattttcggt
    31321 ggttgcagat aaattaatca tgaatccact tgaactttta caacaaaacg agctttttgg
    31381 tgaacttgat tttactttta ttaacaatat tgccaaaaat gcccattatc atcacgcgac
    31441 caagggcgag ctgcttttca tagagggaga gccaggtgat tccttctatt taatagcgga
    31501 tggtcgagta aggcttttca aaagtgccgg caatgaccgg gaaactacta tcaaaacggc
    31561 tctgccaggt gagataatcg gagaagttat tctattcgag agcggcgttt atccggtttc
    31621 ggcacaggcc gttaccgata gtaagctgct ttttcttcat cgttcagagt ttatgggttt
    31681 tttggaagac tcggcgttta gaaatgcttt tttggctgtg ctgatgcgta agcagcgtta
    31741 tcttgctgaa agaataaaag tgctatcttc acttgatgta gaggaaaggt ttctcttata
    31801 tctttctaag cttcctgcgg aaaacgatgc aattaaaatc accgaaaata aaagtgttat
    31861 tgcaggtgaa ataggcacta tacctgaaac tttttcgaga atgatcaaaa agatgaaaca
    31921 acgatcggta ctagagtgga aaggtgatct tgttaaaata gataagaact atatggattc
    31981 ggtactcgaa gagatactga taagctgagg ataatttgag ttcaggtata acagaagaat
    32041 cccgcttgga agataacagg attatccgtt atttgcgtga tgaaaatgta tttactgaag
    32101 aacgggttcg gcatgcctat cgaatgcttt cgaagcaaac acatccggat ataacaggta
    32161 aagacagtag cgattttatt cgccttcgcg aagagtatga gcgagttttg gaaggtatag
    32221 attcgctggc cagatatttt tctgttgagg tttctgcatc tccggcatat gatgaaaaat
    32281 actgccggct gatgttctat ttgagtctta aaagatatat gactgttgga ctctattcag
    32341 taaaactgag aattcgtgag agcgttcggc agcgtaacag gattattctg gaagatgttg
    32401 ttaagcgggc acaggattat gacccctcgt ttatacccct ctttttgaac tataaccgca
    32461 atcttttgcg ccgtttcagc ctctggtata gcagttccgg cagaagaaat aaaaacgggc
    32521 gtaactattt tataaagggc attaattttt ttctagatta cgaactaaga gggagtcgcg
    32581 agatgttcaa cattacgact tcatatctga atgatgctgt ttatgaatta ggtgatggag
    32641 ataatgaccc ggtcaatacc gctatgtacg gaatatcatg ttggcttttg cgcgagctaa
    32701 aacgcaagag cctgtttgac cgctctgcaa tctgaactgg aatataaaaa aaaggattgc
    32761 tctatttttt ggattatgat tgtttaatga ataccaaact agagcaggtg ttaatgaaaa
    32821 agcctcggac tacaatacga aaacgcatga ttttctattt tctcttgatt gctattgcca
    32881 atatatttgt tgcaggcgag ataatttacg agatgaaaag tgaggattat cgcaatattg
    32941 ttgtacaaaa gatgtatcag gtgcagcagg gagttttgga gccggaagtc attttcgaga
    33001 ttattgacca gtggatcagg aagttttcag taatgatttc aattttaatc gcggtatcgg
    33061 cggttattct ttttttgttt gttgtacaaa tagcgtcgcc acttcactac atgattaagc
    33121 aggcagtcaa aatgtccgaa ggcgacttga gtggaatgat aaaggttaat acaaatgatg
    33181 agcttgctga aatggctatg cttgttaacg atatgtctca aaaccttcag gaggttctcg
    33241 cgcaacttga aagaataagc gaggatgttc agtacactgt tcaacttcat tttcgtaata
    33301 ttaatcgcca ttcaaaattg ccagcagttt ttaagaaaga gacactaaat cttcataaaa
    33361 ttcagcagga tatgattttg ctgaaaagct gttttaaact ttatagagtg agtttcaagc
    33421 cggacaaaaa aagcgggaca gacaacagag attagttttg tttttgacag gatattagat
    33481 atttgcgagg aaggtaatta agttgcaagt gcaaccggtt acaaaaatag gggtaccgaa
    33541 atgaaaaagt ttgttttttg tgttgttttc ttgtcattga ttatttttcc ggtttttaat
    33601 gtcggaggcc aggctctcga atcaccggac aaatggttca catataacga taagggagac
    33661 ggtggtgact cgattataaa actttcaaaa ggtaagcaga caataggcgg caaggagtat
    33721 ctggtgatca gctgtaccgg tgaagtaaca aagaagtttg agtatggctt tatcggtttt
    33781 ggtattcgcc tggaagatga aaatttgaag agatttcaaa atgctaaagg tataaagttc
    33841 aaggttatcg gtgatggcaa gtcttatcgt gtacgaactg agtcttccgc tgtgacagat
    33901 tatgattatc atggaaaagt ttttagtgca aagggatccg tgactgaaat atcggtacct
    33961 tattcaagtg ttaaacagga aggttgggga gcgcccaagg gtagttttaa aaagaatact
    34021 cttgttcaaa tttcgtttca gacagtaggg caacctcatg aatctgtatc gcttaaggtt
    34081 tttgatcttc aaccaatgaa ttaaagattg gatttaattc tgtttaatag aaaagtttat
    34141 gtggtaaaaa atatttgtag ggttttaggg gagatatttg gttcagaccc gtcaatttat
    34201 gatcttgttt gtctcttaat gatgaaaggc aagtgaagat tttgacgggt ttttttgtgt
    34261 tcatgataat tcaatataat ctgttgtttt atgtgccagt ttggttttgc aatatgattt
    34321 atgacaagtt gtatttagtt ggaaatgtgt ataggtaatt tgtggaatgt cactgaaatg
    34381 atgttaccct ttcgaaagta gtaattttcc gaagtttcat gtaaatagtg ttgattattt
    34441 gaaagttatt ggttatctgc tcatggttta acagaaaatt atttatgttt tatgtatgat
    34501 ttctgattct tagccagagt gtacataatc caaacaagga gaaagctatg ggaactctgc
    34561 atataggtga atatgaacta atgcctttcg atatcagtgc taaggctgta atggataaat
    34621 atcttgctca gcttgatcta gataccagcg attatacatt tgcggcaaat tatctttggt
    34681 taagtaatgg aacaggtttt tattcaataa ttaatgagac tttctgtttt tttctgctta
    34741 ccagtggtga actctcaatg ctactgccac cagttggtaa gaaagccaat gtaattgatg
    34801 ccatgctagt ctgctttcaa ctgatggatg ataataacag ctctcctttt tattcaaaaa
    34861 tagaatatgt ggacgagata ctagtgtcgg attttatcaa taatcttgaa gatggtgcag
    34921 agatattcga tatgcttgaa gagtatatta ttgagcgtaa acttgttgat tatctttatg
    34981 atgccaacga tcttatcgaa ttgagagggg gctcctactc ctccaagaga aatgagataa
    35041 acaagtttat gagaataaat ccagaccatg ttcttgatac tcttgatgta gaaaaacacg
    35101 gagcagggat acttaaccta gtaaacaaat ggattgccga tcgtatgaaa tatatgccgc
    35161 gcgaagaagc agacgctttt cttgacggaa tatactctga aagaattgcg gttaaacgaa
    35221 tgcttaaaga ttataaggcg ctggatctta caggtcttgt gattattatt aatggagaaa
    35281 tcaaggggtt caccgttgga gaaaaggtta atagtaatac tgccagtgta attattgaaa
    35341 aaaccgattt tgaaatattt ggatgcgcac agtacatttt tagagaattt gttagaatac
    35401 ttgcgaatga ttataatgtt acatctatta atgtaggaga tgatatggga tttgaaaatc
    35461 ttaaaaaagt taaactctct tatcgtccta gcaagctttt accaaaatat acaatatata
    35521 agcgatatta atgatgcaag tatctattcg ttcggctgat aagagtgata taaaagaact
    35581 tgtcgaaata gaaaaatcct cgtttgtgcc gagcgacggg cctttgtcct ctaaatcttt
    35641 tagataccat attgagcgca gcaacattct ttttatagcc tcggttagta ttaatgataa
    35701 atatgttgct gccggatatg ctttggtttt gccaagaaaa aattccatgc gtctttattc
    35761 gattgcggta aatcccgttt acaggggaaa aggggtagca cgcgaacttc taaaagaggt
    35821 gatacaatgc gctgctaaac gtaaagttgc tagtgtttat ctggaggttc gtcctgataa
    35881 tgtagcagcg atacttttgt acaagaaatt cggtttttgt aaaacagccg agttgagtga
    35941 tttttatggc gagggtagcc acggcattag gatgatcgct tatctttagt tggaattaag
    36001 caatttcctg ataagaaaat tgttttttat atgatatttt gcaagtccga taagttcggt
    36061 ctcgttaaca ggttttagta gcagggcaga tattccgttt tctgaaagag agcgcaaaag
    36121 tcgtttttct tttatacggg ttaaaagcaa cagaggcgtt gattctgttt caggatccga
    36181 gtttatttga cggcaaagat cagtggatat tagttcgcag attattatgt cgggattgtt
    36241 tttcttgata acatcgaagg ctttggatga gtcctctatc tgtataatgt tgtgataacc
    36301 gtttctgtta aggtgatttt ttatagttag ttgagtatcg gtttcagggc taataacgca
    36361 aaacagggct ttggtaatat cgtattcttt agacgtgtta aggtttttga tattgtaaat
    36421 aacagattct ttagc
//
LOCUS       DAOTGZ010000006        33261 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_3064
            NODE_1467_length_33261_cov_8.216708, whole genome shotgun sequence.
ACCESSION   DAOTGZ010000006 DAOTGZ010000000
VERSION     DAOTGZ010000006.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868088
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 33261)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 33261)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:22:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,785
            CDSs (total)                      :: 2,751
            Genes (coding)                    :: 2,732
            CDSs (with protein)               :: 2,732
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 12 of 19
            Pseudo Genes (internal stop)      :: 5 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..33261
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1467_length_33261_cov_8.216708"
                     /isolate="AMR_MDS_3064"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(156..848)
                     /gene="lepB"
                     /locus_tag="P5123_00990"
     CDS             complement(156..848)
                     /gene="lepB"
                     /locus_tag="P5123_00990"
                     /EC_number="3.4.21.89"
                     /inference="COORDINATES: protein motif:HMM:NF021981.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006465 - signal peptide processing
                     [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="HRX14868.1"
                     /translation="MNIKNSVPESFKNKFSSLIEYMKGKEFRSNVIFVIFLILIRLFV
                     LGNYSVPTGSMNPTILEGDKFFSNKLAYSLKLPFCKIHPLRWAVPKRGDIIAFLYPGD
                     ESIDYTKRVIGLPGDKILVEGESLSINGQKISKTKIGEDEEFIYYEEDLLGVKHMIQH
                     TKLGHDSIYEKTVPANHIFVMGDNRNNSSDSRVWGFLPIENVTGKLIFRWMSTDPQQF
                     LKIRFERIGRIK"
     gene            complement(1030..3270)
                     /locus_tag="P5123_00995"
     CDS             complement(1030..3270)
                     /locus_tag="P5123_00995"
                     /inference="COORDINATES: protein motif:HMM:NF013727.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015616 - DNA translocase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA translocase FtsK"
                     /protein_id="HRX14869.1"
                     /translation="MFTFLKNSSKKSIPIEQKSRILEQKASFTHIPLHSEIKVHVDAK
                     LPWITKETIVESNSQPYDYLNHISTASPFSTDKTFESSGSFLDEFKKDPDFNMDINEI
                     EIPLSDKRIFMKDVLFDESLLDSNTPAFNEPIEVVNNMQPANAKETTIFDAIAKAPSN
                     RFTLDEDETYKDNFEEMICEQPEETHLEYDTEDENINDKKDILESESLIDQSASLAKA
                     INEPPAKLETPKSTVDTIHVDEKNDKDISQLSAKNLRTESYDDDDECDNYRHKFHTYN
                     VNDEYIIPSYCFSVSQEIDSHIWQKEIERNSVLLVKTLQDFGIESKVTRVNRGPVITL
                     YEIQIAAGIKVNRVSNLSDDIAMALAASRVRIVAPIPGKSAVGVEIPNRSRETVTIGD
                     IFNDSNYQKADMQLKVGLGKDILGKIVMLDLKKQPHLLIAGATGSGKSVCVNTLISSM
                     VFNYDPDRVRFIMIDPKMVELQLYNGLPHQLTPVITEPDIAPKALKWAIYEMERRYRL
                     LSEIHTRDIERYNQKVESYKGQSDREKLPYIVIIVDELADLMMVAPKEIENYITRIAQ
                     KARAVGIHLVLATQRPSVDVITGIIKANFPARIAFQVAQKTDSRTILDQNGAETLLGR
                     GDMLYQSPVSSFPVRIQGAYISEDEIIDLVRHAQKYGNPHYIDIEEEVDKRETVETEG
                     TDAEQDEMFVEAMKIIEETQKASASYLQRRLSIGYNRAARIIERMEELGYVGPQQGSK
                     PRDVLI"
     gene            complement(3427..4266)
                     /locus_tag="P5123_01000"
     CDS             complement(3427..4266)
                     /locus_tag="P5123_01000"
                     /inference="COORDINATES: protein motif:HMM:NF014703.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl-diphosphate phosphatase"
                     /protein_id="HRX14870.1"
                     /translation="MRNTITTFIILGIAQGVAEFLPISSSGHLVILQQIPWIKNSIDS
                     VANNQMLFINVALHLASLAAVIVFFRKDIVKLVTDSVSALFKKNFSDNSLIISTYIII
                     ASIPAAIIGLLFSSYIEDNLMFLLPVSILLIINGLILLYTKRIKRKDRQLEQMGFFTA
                     FLIGCCQAVAVLPGISRAGMTISGGMIRGIESDKAARFSFLMSIPVIAGASLLESVKA
                     FSRGLDSSFFIPLGVGSVACFVSALLALRLLFIIIRKTAFPVFGFYTIAVGLLGVAVN
                     MFF"
     gene            complement(4282..5970)
                     /locus_tag="P5123_01005"
     CDS             complement(4282..5970)
                     /locus_tag="P5123_01005"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR00649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004540 - ribonuclease activity [Evidence
                     IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease J"
                     /protein_id="HRX14871.1"
                     /translation="MKKTNKKSNRKPGTNFKPVKIIPIGGLDAIGRNMTIFERDNTIL
                     VVDCGIMFPTPEMPGIDFVIPDFSYLEQNREKIKGLVITHGHEDHIGAVPFFLKKFMV
                     PVYGSQLTLGFIKKRLEERPVGKEPEFHILEGGDRHEIGCFEVESIPVNHSIVGGMGL
                     AIRSKTGTIVHSGDFKIDHTPEDGRRTDIARFAEIGKEGVLLLMSDSTNAERPGYTGT
                     EKALNSKLFEIFATAKARIIVATFASNISRIQQILNAAQKYNRKVIISGLTMQKNIEI
                     ARSLGYLTYNDSLIAELNEVNTIPLKKQTIICTGTQGEPMSALTRISNGTHKHFKGCT
                     GDTVIITASVIPGNERDIGVVINSLIKQDTVVHYEKSKDIHVSGHASQEELKLMMGLV
                     KPQFFMPIHGEHKHLKAHASLAVDMDIKPSGIIVAENGDVLELTPRYFKKKSHIPIKT
                     VYSEGNYVGDISGDIIKDRQTISADGIVIISIVLAENMLVHKPVVKAKGFSPSLDARF
                     MQRLQEETEYRLDKILGKQVSPEGVSVNLRRSLKSMIFKQTRSNPLIEVYVIEV"
     gene            complement(5967..6401)
                     /gene="dut"
                     /locus_tag="P5123_01010"
     CDS             complement(5967..6401)
                     /gene="dut"
                     /locus_tag="P5123_01010"
                     /EC_number="3.6.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011917485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004170 - dUTP diphosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0006226 - dUMP biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0046081 - dUTP catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dUTP diphosphatase"
                     /protein_id="HRX14872.1"
                     /translation="MINIKIKKSKGAKEPEYQSQGSAGADVFSLLEKDAVINPGDTTL
                     IPTGLFLEIPEGYEVQIRPRSGLALKHGITLLNSPGTIDSDYRGELKIIVTNMGKDSF
                     TITNHMRIAQMVVSRVYRACFCEDTDLSQTERGSGGFGHTGI"
     gene            6797..8620
                     /locus_tag="P5123_01015"
     CDS             6797..8620
                     /locus_tag="P5123_01015"
                     /inference="COORDINATES: protein motif:HMM:NF013961.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglutaminase-like domain-containing protein"
                     /protein_id="HRX14873.1"
                     /translation="MNFIRIILIILILGYGCSSGKSSNHWGEPAQQMEGLPESVIEYF
                     PLKKSEDMVSLSFFFDNETLSKDWIVIGGNPVITNNPAIEGSFSLCISQSLPKATKRG
                     GVSLVLDLPEGDYSVSFYYRNSLDSENGDFFVFEIDGEKKAESTGFSDLWQKHTSVFR
                     SNGEVSMSWYVIGGSGEPNEHLYNTAWIDCVSIVPDQVAKVDIIPRGPQLAVVDETTI
                     EYKAVALRSDNSVKNDIDFDYELEFDGGSGTFNNGVFIGTSSGKVFVTAKTDTLSAVS
                     EPITVLNSNHIEQTVVYNDTNYSGAEITGDGSPQVISDDKIEIALPVYSSFKADAFFT
                     VKGYNHYTGAEKYIFVRVKKGEYEYDTFLSGDFYQRIWLPYGAGIYNVEIYRCTVQAA
                     AVDEEYDGDIFVYSWTNESDILYRFEIENTRNEDGKFLYPSGYIQSDSVEIYNLMQKE
                     SYYLGDDLLKRIVRLHDYTVAFLQYDMDSLTPAQRKKQDALSVLYYKTAVCEGYVSLY
                     SALLRAAGIRTRAVAGTGYQGDSGQLHAWCNVEYNSNLYLVDATWDDPSPNGGNSGFV
                     SRKYLMLETLTGDYGSHEPADERPGRYGSSQSDKFFDISVQ"
     gene            8899..9525
                     /locus_tag="P5123_01020"
     CDS             8899..9525
                     /locus_tag="P5123_01020"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRX14874.1"
                     /translation="MSNTTQSPQTFETKMQNISEYLKGRVLDPAEQEKKNILAEAREE
                     SSRIIEEARKEAEKIVAEAKEHAEREKNSLHSALRIASKQAVDTLKQALEKSLLKQLV
                     AEPVSGVMSSEEVVKSFITEVIKTTDGQSAEIMLSDDLKKKISDYVKSEIITKSSSKI
                     ALSAENIPSGFAVVLSDKQLMLDFAEESLVELLSGFVRSELREYLFEK"
     gene            9535..10317
                     /locus_tag="P5123_01025"
     CDS             9535..10317
                     /locus_tag="P5123_01025"
                     /inference="COORDINATES: protein motif:HMM:NF022415.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2764 family protein"
                     /protein_id="HRX14875.1"
                     /translation="MKLNYYIVGSLPALTWDGDIRGTLQEYLNENGCMLDSFNDGIDN
                     ILLYNEIRTLEMILNNNEAEMFDPSIISKERAIAFLEQPMLNRPDEYPDYIEDYFVRY
                     GDTPSRLENLNELYLDYFTTLCDSNIPFFNFFGKTALIIRTIVSAYRLQSKNISLDEK
                     LVGDEEVVQAIIAHKNSADFGLAQVFPEIGRLQSIMEKNTLEKEKELDLFLLDLLSEY
                     RADDPFGDHVIYLYIYSLFLRDRWVMLDSTTAESLVQEIIQG"
     gene            10333..12093
                     /locus_tag="P5123_01030"
     CDS             10333..12093
                     /locus_tag="P5123_01030"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013181698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit A"
                     /protein_id="HRX14876.1"
                     /translation="MALEKATGKIVAAQGNLMTVEFTGIIRQNELVYVKTGGQSLKGE
                     VIEINGNLAKVQVFEPTRGVRYGDLVEFDEELLSVELGPGLLKSIYDGLQNPLEDVAK
                     EAGYFLPRGMYLTPLDREKKWSFTPMVKVGDPVERASYIGWVPEGVIEHKIMVPFRFQ
                     GKGTIVEIKDAGEYTIDDEIAKIKDENGNVQSITMAQKWPSKFPLRFGKRRYPDQPII
                     TGSRIIDGPFPVAKGGTFCTPGPFGAGKTVLQHQISKNAEAQVVIVVACGERAGEVVE
                     ILREFPLLDDPQTGKKLIDRTCIICNTSSMPVAARESSVYVGTTIGEYYRQMGLDTLL
                     LADSTSRWAQALREMSGRLEEIPGEEAFPAYLSSRIAEFYERAGLIENVMGDIGSLTM
                     GGAVSPAGGNFEEPVTQSTLAVVGCFLGLSRSRSDARRFPAIDPLISWSKYFDRFGET
                     GDDDLKKKVKIIHKAQTVLKESDEIGKKMSVVGEEGISLEDIVVFLKGEFYDSVFLQQ
                     NAFDKVDTTCDVNRQLELAEVVGTVIDTKFKFNTRDDARKFFVDLSNQVIQWNYTAKG
                     DPDYKVQKNAILEQVRGEKE"
     gene            12090..13391
                     /locus_tag="P5123_01035"
     CDS             12090..13391
                     /locus_tag="P5123_01035"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002669361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit B"
                     /protein_id="HRX14877.1"
                     /translation="MIKAYNKIERIKGSIAQVKAEGVFLGELAQVKMKSGGSTLAEVI
                     SFDGDRVNLQIYQGTKGVSTGDQTRFIGKPMQVRFGDSLLGRVFNGSGDPIDEGPEVM
                     EESVDIGGPSFNPARRIIPRRMIKTNIPMIDMFNTLVISQKIPIFSIPGEPYNELLAR
                     IASQTDADIIVLGGMGLKYDDYEFFRQYFTKSGAMEKTCMFVHMASDPVVECLLVPDM
                     ALAVAEQFAVQDKNVLVLLTDMTSFADAGKEISISMEMVPSNRGYPGSLYSDLASRYE
                     KAVDIDGSGSITLIAVTTMPGDDVTHPVPDNTGYITEGQFYLHNGVIDPFGSLSRLKQ
                     QVMGKVTREDHGDLANAMIRLYADAKKARDRESMGFRLSEWDQKLIKFSVMFEERMMD
                     LGVNLDIEDQLNLGWEILASCFGKNETGIKNEWIQKYGKWS"
     gene            13404..14024
                     /locus_tag="P5123_01040"
     CDS             13404..14024
                     /locus_tag="P5123_01040"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: protein motif:HMM:NF002565.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit D"
                     /protein_id="HRX14878.1"
                     /translation="MAEIKLNKTELKSQQMLMDQLSKYLPTLQLKKQQLQVEVNSVKN
                     YFDELNQKMDKMKNDFLSWSILLSQREPVDIKEFLAVEEVISLQENIAGVEVPFFDKI
                     IFTDREYSSFSTPLWFDKAIESLKELITVREQMRITEIRQQLLEEELRQTNIKVNLFE
                     KRLIPRCKNNIKKIKIFLGDQEIASICNAKIAKDKLKRKELTAEAV"
     gene            14025..15908
                     /locus_tag="P5123_01045"
     CDS             14025..15908
                     /locus_tag="P5123_01045"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRX14879.1"
                     /translation="MIQRMQKVLFVGPESKRENFLKKLQKAGVLQIDDYTGSKFNKSD
                     NFVDTTKTDRIVQSWKLLKQYQTLAENIMHHNKKTDNISAVVDDVLAIERELGDLYDE
                     QKSLESKIAMIEPLGNFDLDVIRNIENLSSSMIQFWEVSEKNYSRLDIPKKAFVIDVQ
                     NRGGRIYFITIYHKVLQIDKTSEIVITESLKELQYQLVNNERKQSEFKNKLIDFTVYL
                     DDIHLLYLSELNELNYKKALGAMITPLDGMLFALQGWTSKEDLDSLRKLSDNNEAELI
                     EIEPDKGEKEPTLLKNKGLGEMGQDLVLFYDTPSSKEWDPSTWVFLSFIVFFAMIMGD
                     GGYGITLFSLLLFFRIKNRKASASTKRFLNMGMVLTFATFIYGALFDNIYGVNFFIPK
                     GLSFPEFMLPAVEWINNFKGLESAQKNSALMKTSVLIGMVHISLSLILKVLRDFSNRV
                     FIKPLSNIAWIAIIWAFYFWYSSGAQTSGLLQTTPGLVLTAGLGIVFLTSAGTLHPGK
                     LIVGGLGGIYNGVQFFSDVLSYIRIFALGLSGSLIAVVFNDLAGMVFNSGMNIFLAGL
                     LGALILIFGHLLNIGLCLMGAVIHGLRLNFLEYYRWSFDGDGRPFKPFSDLLLRGVAA
                     KNQ"
     gene            16027..16446
                     /locus_tag="P5123_01050"
     CDS             16027..16446
                     /locus_tag="P5123_01050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010882742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP synthase subunit C"
                     /protein_id="HRX14880.1"
                     /translation="MINAQFVGAILALGLGAIGSSIGCGLAGMTSHGVMAKVDEGHGL
                     ILGLSAMPASQTIYGFVLMILLQGSTVQASTAFGIGLGAGLAFMTSAIYQGKACSSAI
                     LGVSKKSSISGKVFLAPGIVESFALFALVGGIVLLQG"
     gene            complement(16636..17589)
                     /locus_tag="P5123_01055"
     CDS             complement(16636..17589)
                     /locus_tag="P5123_01055"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRX14881.1"
                     /translation="MKLSQDIIESVFRQIETRNHASNNQAIIHSDELKNILSAQLGVS
                     KDIIAQVVSILLDAHKILHIEIIAEDTARNIDRIDGFVVTELSVIRNLKSYFEKQLII
                     LYEKQYHKSMGAASIIKELFGQMHTINMTEIGQILNKTIILGEYEKMIEKDFTTYSHE
                     WQEKKMIELAVERGFRWHSDIPELNEENKSLETDVKIESADNDDQTISAIIPEGMRAV
                     DTEEYTDFTDKSQKYPLNRILNIYGIDFFTKIYFRRYKFGYIQQLIEDRQITRKSDLF
                     QIKEILAVVKKNILKDRALTDHREEIYALDKAITHAMFIHN"
     gene            17760..18443
                     /locus_tag="P5123_01060"
     CDS             17760..18443
                     /locus_tag="P5123_01060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020886144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:1990397 - queuosine salvage [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="queuosine precursor transporter"
                     /protein_id="HRX14882.1"
                     /translation="MSNEVLWIVFLIVDLLFTLITFKIFGKKGLFALIAANIILCNIQ
                     VAKTIELFGFTVTLGNILYGSIFLSTDLLSEFFGKKEARQGVIIGFFFMLFMTVVMQL
                     ALFFVPTEEGLVVHEALTVIFSVMPRIAAGSLAAYLVSQLHDVWAFNFWKVKTGGKKL
                     WLRNNLSTLVSQAIDSLIFCFIAFWGMEQSILIEIIITTYIMKVFVAIADTPFIYIAK
                     KICPSDQIA"
     gene            18485..19339
                     /locus_tag="P5123_01065"
     CDS             18485..19339
                     /locus_tag="P5123_01065"
                     /inference="COORDINATES: protein motif:HMM:NF024118.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HRX14883.1"
                     /translation="MKKISAKLKFWGVRGSIPVPGNDFACYGGNTSCVELRCGETIIV
                     LDAGTGIRPLGLSYLKEFGDKPVHFNILISHSHWDHLQGFPFLSHIYIPGNSVTFYGG
                     HTVNTLEKLVMTQLSEEFHPKQLYELSADIKFVELKKNNFSINDVQISFTHLLHPGLS
                     LGYRICYKNHVFAYVTDNEILADEKMAHHNWENIGSIIKGADVVIHDAQYTDEEYKSK
                     LGWGHSSIENVVKICNKFRVKNLYAFHHDPLHTDKDIKKMIKNARKLANKDLKVYAAR
                     EGVELLFY"
     gene            complement(19343..20434)
                     /locus_tag="P5123_01070"
     CDS             complement(19343..20434)
                     /locus_tag="P5123_01070"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRX14884.1"
                     /translation="MKKALPLLLLICFSCSKTPEEKFQEAVLKYNESKDPEARLVIEE
                     NYFNSLGAVQIDKADNYIAGHNCIILEKESLTTIITSDGFKEPWSEDILHASYNPETK
                     VLCTSDGYKAEFFVFGRNEETQEQTVKRVNTIELTKDEEKPIDAFYVEKDSLYYFYNN
                     RIFTADISSGEANNLLPDTTFSAPFKKDQFRVSLKIRSDHLAVCIGNAGSYNLSLYSL
                     TTNKKVFQNKKTTSIFFALLNDRILYIGGKTGAWVIYRHFFTYDKPEQVILFKTLRDI
                     SISGETIFYVDADDFHITDTFFTKQLSEKGLYSIKRLINDYLLIAHNGTLYLVQTKKY
                     FDTNKHFEELVPDFFEVEEISAEDISDNG"
     gene            complement(20431..21804)
                     /locus_tag="P5123_01075"
     CDS             complement(20431..21804)
                     /locus_tag="P5123_01075"
                     /inference="COORDINATES: protein motif:HMM:NF024299.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HRX14885.1"
                     /translation="MNIVNITLFFIIVSLISSFVYYYKTMRLPARVRRAQEMIKNGND
                     KEASEIIKTVLNKKKDYVPARYLRAQMLHQQGQFVLAISEFNAILQTPDFSRHVNELK
                     IHYELAELYNSTKAWKKEIDEYKIILTFNPDDINANHRLGLSYYKQNRYKDAKDALMR
                     AINSDPSLDDCYLPLGISCYQIADYNNAENFLLKAIERPYKQEEAHYYLGLILKGKKD
                     YDNAIISFERSKTDSKLFRKSLMRIGEIYFETANYDQAITTLETGLSSLKPRDEESIE
                     FRYLLAESYEMDNKVQEAVHHWEKIQSEQPNYRSTQIKLDEYKALLEDVYMKQIFTSS
                     FDALQPLLGEIIARLNYNIISKTFLNKNQAYYKVYHTKRINEPPILIFFARSTHEISE
                     NDINFFSRLLRDEKCKSGIYITTSRYGLKAKSAATAKSIELYAKDFLSKSIEKIKGKA
                     KVKGSET"
     gene            complement(21813..22280)
                     /locus_tag="P5123_01080"
     CDS             complement(21813..22280)
                     /locus_tag="P5123_01080"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRX14886.1"
                     /translation="MSNNFGYPDQEVLIRALATYTSTKVSKSISIIRGMIRMSIEAIC
                     EKNKTTLKEVKLLEEQERDSFYMDTMVYFFEKMNLDNITRKKMKYDFLQIYERWKESK
                     REEIDIVDPDKALSLKSTDVPADTINDSVLDTTESIDFIDNLDSLDDFDIFSN"
     gene            complement(22625..23692)
                     /locus_tag="P5123_01085"
     CDS             complement(22625..23692)
                     /locus_tag="P5123_01085"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRX14887.1"
                     /translation="MRKIYLSIIILLLPVFSVQSGTVTVTEGSYATPEITDTINEQMS
                     EIAEDINEDSIIMLSGKQNNLAQATHNAAGGLFLNGSLFSAMDSRLGSVSIGFAAGAE
                     NPFVLEEFPNNIRQGYDKKSSFAVSAFTVDCNVNADNLPFIKIKGIVFNTKIGYFKAD
                     SLITDVKFDSFLFGVGARYKLFEIPVQNPFFQLRSLTAGTGIYYSSATLSFFSDTLEK
                     EITADNGITTESSTDVSFQMDTYNVVTPIDLVTTANAFHFLNIIAGFGVDLNLGSSTL
                     NADADIKTSSFDDQGNPITSDNPAKLNLTDSKTDDHGSYLRYKMIFGLGFEFGPGRIE
                     IPAVFYPGNGWAASISIGASI"
     gene            complement(23961..26549)
                     /gene="clpB"
                     /locus_tag="P5123_01090"
     CDS             complement(23961..26549)
                     /gene="clpB"
                     /locus_tag="P5123_01090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015948179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent chaperone ClpB"
                     /protein_id="HRX14888.1"
                     /translation="MRMDKLTIKAQELIALAQENATKSDHPEVQPIHLLTALTQQDGS
                     IFTSMCQKIGVPVDLIATECTEAVKKLPRVTGAGPGGGSLSQTFREIMNTAWNEAQKL
                     QDKFLSSEHLVLALTLVKNTASYKILHANGFNYENVLRVLSEIRGNRSVNDENAEDKY
                     NALEKFARDLTRLARQNKLDPVIGRDDEIRRVMQVLTRRTKNNPVLIGEPGVGKTAII
                     EGLAQRIVSGDIPENLRDKRVVSLDMGALIAGAKYRGEFEERLKSVIEEVEGSAGEVV
                     LFIDELHTLVGAGASEGSVDASNMLKPALARGELQCIGATTLDEYQKYIEKDKALERR
                     FQPVMTSEPSVEDTIAILRGLKERYEVHHGVRISDAAVVAAAILSDRYITNRFLPDKA
                     VDLIDESASLIKMEIDSMPIEIDEIERRSRQLIIEREAVKKEKDATSKEHLDKIEQEL
                     ANLNEKKSTLMAQWQNEKEIITGVRRAKEEIEHLRNEEQQAERAGDLNRVAEIRYGKI
                     VELEKYLQVQQLALSEIQSQKKLLKEEVGEEEIASVVARWTGIPVSKMLEGETQKLLK
                     MESLLEKRVVGQHQAITAISDSVRRSRSGIQDPDKPVGTFIFLGPTGVGKTETAKAVA
                     EFLFNDERAIVRIDMSEYMEKHAVARLIGAPPGYVGYDEGGQLTESVRRHPYSVILFD
                     EIEKAHPDVFNIFLQILDDGRLTDSKGRTIDFRNTIIIMTSNIGSTYISDESIPIETR
                     NENVHRELKMHFKPEFINRVDETIIFNPLSKDHIAGIVKLQLVMLAQRLKKQQNIELE
                     ISKDALAILAKKGWDPQFGARPLKRTIQRELLNPLSLKILSREILPGSTVKISSKGDG
                     ILINTV"
     gene            26790..28409
                     /locus_tag="P5123_01095"
     CDS             26790..28409
                     /locus_tag="P5123_01095"
                     /inference="COORDINATES: protein motif:HMM:NF012730.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006811 - monoatomic ion transport
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ion transporter"
                     /protein_id="HRX14889.1"
                     /translation="MINPDSRFKIIWEVYILLLTIVVAVQAPLMIVFEINPKGILFFF
                     DMIISLSFMVDIVLNFNTKIIEKRRVIEDRKGVTKAYLKGWFWIDFLATIPFDIFYVT
                     FQLGSLSRILRLFRLARLFRLLRLIRIAQTMKRVNNSNLFNPSVLRLVFMVFWIFFAA
                     HFIASAWIVILGNPNNLDNVTRYIRSVYWTVTTLTTIGYGDITPVTNAQTMFVIIIEI
                     MGAGMYGFIIGNIANLIANIDIAKAQYKEKLEKINTFMKYRNLPSPLQNRINEYYNYL
                     WESRRGYDESSVLLDLPISLKTQVALYINKDIIAKVPIFKGAEEAFIKEVILNLEPVV
                     FTPGDTIVYKGDIGFDMFFISKGSVDVVSEDESIVYATLTTGQFFGEIALLFSSPRTA
                     TIKAREYCDMYRLKKEIFDDILERYPDFEKQIKELADKRKAEIMAEQIGINKNIKTEV
                     VDSPDTVMNLRAERSENGITLSWNGPHGEIPCHYEIIRKQSDNRWKILDSSVKVEIFS
                     DKDADPDEVNVYKVRAVNVGGPGPWSNSVKI"
     gene            complement(28641..28904)
                     /locus_tag="P5123_01100"
     CDS             complement(28641..28904)
                     /locus_tag="P5123_01100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020585599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRX14890.1"
                     /translation="MQNLDARDFELPPSTVLQQVTSDHIAIVKIVKSRIIMKDGEKIL
                     QIADKIRKKLPYTTVSLRTTAPVCSKTEEFLKQNGIMIQKSLF"
     gene            29133..30521
                     /locus_tag="P5123_01105"
     CDS             29133..30521
                     /locus_tag="P5123_01105"
                     /inference="COORDINATES: protein motif:HMM:NF014385.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TolC family protein"
                     /protein_id="HRX14891.1"
                     /translation="MKKLVALLLFFVFTINLYSSSELDYDIYLGFVKNNLPELIKTRL
                     NEEIARREVQKASGINDTKINGSLYYYKNDPTQVQQSQGVEDTTGLTGEAEVTKKILP
                     SGTELFGGAALSRSVVDRDPQQTAYQPSLTVGVRQPLLYNFLGSVDRFSEQNARLGYE
                     IERIQTKITENSLLNSYALLYHQWIVYSRIAEINRKGVVNARTVYNQMLRKKRSGLAE
                     NDEVQKSYSSLLSFRQKHEQSMIDEIQVREKIAVLTGLDSIKPESSQFDRQYSLVKEF
                     SFDLRNFNTTTSSKYIDLLAERMKLSRETAENKLMPQVDAVASVTKWDESDSFSDSVS
                     YGKTDYKIGVELSHSFGNNSAKAEYEISKLEIVKIQEDMRSAINSFNTEIRQMRDESE
                     RIKTIIIYKERNIRALRSQLATETQKYQSARIDLAYLINTRNSIAAEESSLLLQKYNL
                     ISLYYAYSELVE"
     gene            30605..30970
                     /locus_tag="P5123_01110"
     CDS             30605..30970
                     /locus_tag="P5123_01110"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRX14892.1"
                     /translation="MAGNKSRSLRNMKIVSLIDYRQKKLKDQYDQDCLDEENFIMESI
                     FELARDYDDEREWRNNYILNIINSDEFVDDYFFGREWNENDRLVKYLLMMMPGYNPEK
                     LYTSNDIDLLMVEGKFPVD"
     gene            31046..31651
                     /locus_tag="P5123_01115"
     CDS             31046..31651
                     /locus_tag="P5123_01115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013433103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fumarylacetoacetate hydrolase family protein"
                     /protein_id="HRX14893.1"
                     /translation="MNPSKIVCVGLNYKRHAAELSLSVPEEPVIFLKPPSSMIGSGDL
                     IHYPDKVTRVDYEAELGVVIHKTCRNVDKDSALRYIYGYCCVNDVTARDIQTKEVQWT
                     RAKSYDTFCPVGTIVKKENVDPSNLKICSYVNDEIKQNSNTSDMIFTVAECISFISSI
                     MTLLPGDLILTGTPEGIGPLNRGDQVRVEIEGFPVLINSVS"
     gene            31913..>33261
                     /locus_tag="P5123_01120"
     CDS             31913..>33261
                     /locus_tag="P5123_01120"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: protein motif:HMM:NF012492.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HRX14894.1"
                     /translation="MMTFDTMGLGPLILDAIDNLGFATPTPVQEKVIPFVLQNRRDLT
                     ALAQTGTGKTAAFGLPILEQVSENKNVQALILAPTRELCIQISKDMLSFARFKPEINI
                     VPVYGGEKIELQINKLKRGAQIVVGTPGRLNDMLRRKYLDVSSIKWLVLDEADEMLTM
                     GFREELESILMTTPDTKQTLLFSATMPDSLKDNFMKNPEEIVIGKRNAGNTNIEHRVY
                     MVKSPDRYLALKRVVDNAPDIYGIVFCRTRNETKEVSDKLIKDGYSCDALHGELTQAQ
                     RDHVMKRFREKHLRLLIATDVAARGLDVNDLTHVINYNLPDDVEVYTHRSGRTGRAGK
                     TGISAVIVNTREQRKIEQIERTMKIHFERALVPGGREICETRLFDLISRVEEVKLKEE
                     QINSFIEPIIEKLSVFERDELIKRFVSVEFNTFLEYYKNAKDINAKSTGNSRDRGSG"
BASE COUNT         9993 a         5765 c         6753 g        10750 t
ORIGIN      
        1 caaataaaaa aattaccaaa aaagtttcag ttagtatgat tgaacttcgt aatgaaagtg
       61 aatgtgataa gtttatacaa aaagtaatgt cactatacta aatatcagaa atatccctta
      121 aaaagtatca actcttttaa agggatattt tttgattatt ttatacggcc gattctctca
      181 aatcttattt taagaaattg ctgaggatct gtagacatcc atctaaaaat caattttccg
      241 gttacgtttt caattggtaa aaatccccag actctgctgt cagagctgtt gtttcgatta
      301 tcgcccatta caaatatatg attagctgga acagttttct cgtaaatgga gtcgtgtccc
      361 agtttagtat gctgaatcat atgttttaca cctaaaagat cttcttcata gtagataaac
      421 tcttcatctt caccaatctt tgttttggat attttttgtc cattaattga cagactttcg
      481 ccttctacca aaattttgtc accaggcaaa ccaataaccc tttttgtgta atcgatgctt
      541 tcgtcaccgg gataaagaaa cgcgataata tctccgcgtt taggtacagc ccaacgaagc
      601 gggtgaattt tacaaaaagg aagcttaagg ctataggcaa gcttattaga gaagaattta
      661 tcaccttcca gtattgtagg gttcattgat cctgtgggta ccgaatagtt acctagaaca
      721 aaaagacgta tcagaatcag aaaaattaca aaaataacat tgcttctgaa ttctttacct
      781 ttcatatact ctattaatga tgaaaacttg tttttaaaag actcgggaac cgaatttttt
      841 atattcattt gtaacctctg ataatttact ggtttgtaaa cagccggaat aataaaaaaa
      901 gttaaagcct ttttcatagg ctccataata aatcaataat taagatacct ataagcttta
      961 ccatattgaa tcagtcaata ataaatagaa tttgatttag gatggatatt tatgtcaata
     1021 ctgtattaat cagattaata cgtcacgagg cttgctgccc tgttgtggcc ccacatagcc
     1081 gagctcttcc atgcgttcga ttattctggc agcccgattg tacccaatac tgagtcgacg
     1141 ctgaagataa gaggccgatg ctttttgagt ctcttcaata attttcattg cctctacaaa
     1201 catctcatcc tgttctgcat cagttccttc tgtttcaacg gtttctctct tgtcgacctc
     1261 ttcttcgata tctatatagt gtgggttgcc gtatttttga gcatgacgaa caagatcaat
     1321 tatctcatct tccgagatat aggctccctg tatacgtacc ggaaaagagc tgacaggtga
     1381 ctgataaagc atgtctcctc gtcccaatag tgtttccgcg ccgttttggt caagaattgt
     1441 gcgtgaatca gttttctggg caacctgaaa agctatacga gcaggaaagt ttgccttaat
     1501 tattcctgta attacatcaa ctgatggacg ttgtgttgca agcacaaggt gtatgccaac
     1561 agcacgagcc ttttgagcta ttcgagtgat ataattttcg atctccttgg gggcaaccat
     1621 catcagatcc gcaagctcat ctacaataat cacgatatat ggtaatttct cgcggtcact
     1681 ttgtcctttg tacgattcaa ccttctgatt ataacgttcg atatcccttg tgtgaatttc
     1741 ggataataac ctataacgtc gttccatctc atagattgcc cattttaacg ctttgggagc
     1801 gatatcaggt tctgtaatta ccggagtcag ttggtgcgga aggccattat aaagttgcag
     1861 ttcgaccatt ttggggtcta tcataataaa ccgaaccctg tcagggtcat aattaaaaac
     1921 catgctcgat atcaatgtgt taacacatac agattttcct gatcctgtgg ctccagctat
     1981 aagtaggtga ggctgttttt taaggtcgag catgactatc ttgcccagaa tgtcttttcc
     2041 cagaccgact tttagttgca tatccgcttt ctgatagttt gaatcgttaa agatatcacc
     2101 gatagtaacc gtttctcgtg aacgatttgg aatctcgacc cctacggctg attttcccgg
     2161 tattggtgca acgattcgta cccgagatgc tgctaaagcc attgctatat cgtctgataa
     2221 atttgaaacc ctgtttacct tgatgccagc cgcgatttga atctcataga gtgtaataac
     2281 tggccctctg ttaacccttg ttacttttga ttcaatacca aaatcctgta aagttttaac
     2341 aagaagtaca gagttacgtt caatttcttt ttgccaaatg tgtgaatcta tttcttgaga
     2401 tacagaaaaa cagtaagacg gaataatata ttcatcatta acgttatacg tatgaaattt
     2461 atggcgataa ttatcgcatt catcgtcgtc atcataggat tctgttctaa ggtttttcgc
     2521 ggacaattgt gatatatctt tatcattttt ttcatctaca tggattgtat caaccgtaga
     2581 ttttggtgtc tctaatttag ctggaggctc attaattgct ttcgctaaag acgcggactg
     2641 atcaatcaga gattcacttt caagaatgtc ttttttatca ttgatattct catcctctgt
     2701 atcgtactca aggtgtgttt cttcaggttg ttcacagatc atttcttcaa aattgtcttt
     2761 ataagtctcg tcttcatcaa gagtaaaacg atttgaaggt gctttggcta tggcatcaaa
     2821 aatggtggtc tcttttgcat tagccggttg catattgtta acgacttcta ttggctcatt
     2881 gaaagccggt gtgtttgagt caagaagaga ttcgtcaaat agaacgtctt tcatgaaaat
     2941 acgtttatcg cttaagggga tttcgatttc gtttatatcc atattaaaat caggatcttt
     3001 tttaaattca tcaagaaagc tcccactgct ttcaaatgtt ttatctgtag aaaaaggtga
     3061 tgcagttgaa atgtgattaa gataatcata tggttgagaa tttgattcta caatggtctc
     3121 ttttgttatc cagggcaatt tggcgtctac atgaacctta atctcgctgt gcaaaggtat
     3181 atgagtaaag gaggcctttt gttcaagtat ccgggatttc tgctctatcg ggatactctt
     3241 ttttgatgag ttttttagga aagtgaacat ggaaactcct cgggttttga ttgataaagg
     3301 tatacctgaa aaatagagac ataccacact aatgattatc gaaagaaata ggattaccct
     3361 tgagcttgtt atttttgaaa aaatgaacca aaatagagaa ctatataacc gcctgagttt
     3421 tcgggatcag aaaaacatat ttaccgctac tcctaaaagt ccgactgcaa ttgtataaaa
     3481 accgaataca ggaaaagcag tctttctgat tataataaat agaagtcgca aagccaaaag
     3541 agccgaaaca aaacaggcta cagaaccaac accaagcgga ataaaaaatg aactatccag
     3601 tcctctcgaa aaagctttaa cagattctag cagacttgct ccggcaataa cgggaataga
     3661 cattaaaaaa gagaagcgag cggctttatc tgattcaatt ccgcgtatca ttcctccgct
     3721 tattgtcata cccgctcgag atattccagg caatacagct actgcctgac agcatcctat
     3781 caaaaacgcg gtaaaaaatc ccatctgttc taattgtcgg tcttttcttt ttatccgttt
     3841 agtatataat agtattaaac cgttaataat tagtaaaatt gaaacgggta aaagaaacat
     3901 caaattgtct tcgatatatg atgaaaaaag aaggccgatg attgccgccg ggatagaggc
     3961 gataatgata tatgtactga ttattagaga attatcagaa aaattctttt taaagagagc
     4021 gctaacagaa tctgttacca gttttacaat gtctttgcga aaaaaaacta taacagccgc
     4081 aagtgaggct aaatgaagag ctacgtttat aaaaagcatc tgattattgg caacagaatc
     4141 tatagaattt ttaatccatg gtatttgctg taaaattacc agatgtcctg aagaagatat
     4201 cggcaagaat tcggcaactc cctgtgcaat accaaggata ataaaagttg tgatagtatt
     4261 tctcattaat ggactccagc gttaaacttc gattacataa acttcaatta gagggttgct
     4321 gcgtgtctgt ttaaaaatca tcgattttaa acttctcctc agattgacag aaacaccttc
     4381 tggtgaaacc tgtttcccaa gaatcttgtc aagtctgtat tcggtctctt cttgcagccg
     4441 ttgcataaat cttgcatcaa gtgaaggtga aaacccttta gcttttacta cgggtttatg
     4501 aactaacatg ttttccgcaa gaacaatgga tattatcact attccgtctg ccgatatagt
     4561 ctgccgatcc ttaataatgt caccagaaat atcgcccaca taatttcctt ctgaataaac
     4621 tgttttgatt ggaatatgac tctttttctt gaaatagcga ggagttagct ccaaaacgtc
     4681 gccgttttcg gcgacaataa ttcccgaggg tttgatatcc atgtctacag caagtgaagc
     4741 gtgtgctttt aaatgtttat gctcgccgtg tattggcata aaaaattgtg gtttcacaag
     4801 tcccatcatc agtttcagct cttcttgtga ggcatgaccc gatacatgaa tatccttgga
     4861 tttttcatag tgtactactg tatcctgttt tatcagtgaa tttattacga cccctatgtc
     4921 cctctcgtta cctggtatta cggatgctgt aattattaca gtatcgccgg tacaaccttt
     4981 gaagtgttta tgagtgccgt tagagattcg agtcaaagct gacatcggtt cgccctgtgt
     5041 tccggtgcaa attatggtct gtttttttaa aggaattgta ttaacttcgt tcagttcggc
     5101 tatcagtgaa tcattatagg taagataccc caaacttctg gctatttcga tattcttttg
     5161 cattgtaagg ccggaaataa taacctttcg gttatatttc tgcgccgcgt taagtatctg
     5221 ctgaatacgt gaaatatttg aggcaaatgt tgcgacgatg atccgagctt ttgccgtagc
     5281 aaagatttca aaaagtttgc tgttaagtgc tttttctgtt cctgtatatc ctgggcgttc
     5341 agcgtttgtg gagtctgaca taagtagcag tactccctcc ttgccaattt cagcgaatct
     5401 tgcaatatct gtgcgccggc catcttcagg agtgtgatct attttaaaat cgcccgagtg
     5461 aacaatagtt ccggttttag acctaattgc aagacccatt cctcccacaa tagagtgatt
     5521 tacagggata ctctcaactt caaagcatcc tatttcatga cggtcaccgc cttccagaat
     5581 gtgaaactcg ggttccttgc caaccggacg ctcttcgaga cgttttttaa taaatccaag
     5641 agtcagttgt gatccataaa caggaaccat aaactttttc aaaaaaaagg gaacggctcc
     5701 aatatggtcc tcatgtccgt gtgttataac cagacctttg attttttccc ggttttgctc
     5761 caaataagaa aaatctggga ttacaaaatc aattccaggc atctctggtg ttggaaacat
     5821 gattccacaa tcgacgacca aaattgtgtt atcacgttcg aaaatagtca tgttacgccc
     5881 tattgcatcg agtcctccaa tagggatgat ttttaccggt ttaaagttgg taccgggttt
     5941 tctgttgctt tttttgttag tttttttcat atacctgtgt gtccaaaacc tccggagcct
     6001 ctttctgttt gtgaaaggtc tgtatcttca caaaaacagg cacggtacac cctggaaacg
     6061 accatttgag ctattcgcat atggttggta atagtaaagc tatccttgcc catattcgta
     6121 actatgatct ttaattctcc ccggtaatca ctgtcgatag tgccgggaga atttaacagg
     6181 gttattccat gcttaagagc taaaccgcta cgcggtcgaa tctgtacctc atagccctcc
     6241 ggtatttcca gaaaaagtcc ggttggtatt aaagttgtat cccctgggtt gatgactgca
     6301 tctttttcaa gtaaagaaaa aacatcggct cccgcggatc cctgactttg atactccggt
     6361 tctttggctc ctttggattt tttaatttta atattaatca tataattatt atagggaatg
     6421 ccgcaattat tctgttacag aaagtgagac cgttgaaaca gaaaatattt aagctcccaa
     6481 ttttgttcca tttatttcaa aataacctga atccaggatt ggtactaatc agtagtattt
     6541 tttataataa taaccataaa aaatatcata aattagttca tcctgctttc gaaggcaaca
     6601 aaaaagtgta tatttgatgt aactctatgc cagatcaagg tttaagtata aataatagtt
     6661 ttatttgctt atttataaat taaattgtgt aaaagactat tggtaattat tacaacagtt
     6721 tgtcaataga aacggttaag aaggcatgca ataattgctt tttaagccat gtttaaccaa
     6781 atacgggaag attttaatga attttataag aattatttta attatactta ttcttggtta
     6841 tggatgttcg tcaggaaaaa gctctaatca ctggggagaa ccagcccagc agatggaagg
     6901 acttccggaa agtgttatag agtattttcc actaaaaaaa agtgaagata tggtttcttt
     6961 atcgtttttc ttcgataacg aaaccctatc gaaagattgg atagtaattg gtggtaatcc
     7021 tgtgataacc aacaatcctg ccatagaagg gtcgttttcg ttatgtataa gccaatcgtt
     7081 gcccaaggca acaaagcgag gtggtgtttc ccttgtttta gatcttccgg aaggtgacta
     7141 ttcggtctct ttttattatc gaaacagtct tgattctgaa aatggagact tctttgtgtt
     7201 cgaaatagac ggtgagaaaa aggcagagag tacagggttt tcagatttgt ggcaaaagca
     7261 tacttctgtg ttccgttcaa atggtgaagt tagcatgagt tggtatgtga taggcggctc
     7321 gggtgaacca aatgagcatc tttataatac agcctggatt gactgtgtca gtatagtccc
     7381 tgaccaggtt gccaaggtag atattattcc aagagggcca caactggcag tagtagacga
     7441 aactaccata gaatataagg ctgttgcctt gcgtagtgac aattctgtga aaaatgatat
     7501 tgattttgat tatgaactgg aatttgatgg tggcagtgga acatttaata atggggtttt
     7561 tatcggaact tcatctggaa aggtttttgt gactgcgaaa acagatacat taagtgcggt
     7621 ttcagaacct attacagttc taaatagtaa tcacatcgag caaacggttg tatataacga
     7681 caccaattac tcgggtgccg aaattaccgg cgacggatct ccgcaagtta taagcgatga
     7741 caagattgag atagcgttac cggtttattc aagttttaag gcggatgctt tttttacagt
     7801 taaaggttac aaccactata caggagctga gaaatatatt tttgttcgtg taaaaaaagg
     7861 tgagtatgag tatgatacat tcttaagtgg tgatttttac caacgaatat ggctccctta
     7921 tggcgctggg atttataatg ttgagatata cagatgtacc gtgcaggctg cggctgttga
     7981 tgaagagtat gatggagata tttttgtata tagctggaca aatgagtcag atattctcta
     8041 tcgttttgaa attgaaaata cgcgaaatga agatggaaaa tttctttacc cttcaggata
     8101 cattcaatca gattctgttg aaatatataa tttgatgcaa aaagagagtt attatcttgg
     8161 agatgatctt ttaaaacgta ttgtaagact tcacgactat acggtagcat ttttacaata
     8221 cgatatggac tctttgactc cggctcaaag aaagaaacag gacgcgcttt cggtacttta
     8281 ttataaaaca gctgtttgtg aggggtacgt ttcattatat tctgccctcc ttcgggctgc
     8341 gggaataaga acacgagcgg ttgccggtac tggatatcag ggcgactctg gtcaattgca
     8401 tgcttggtgt aatgttgagt ataacagcaa cctatatctt gttgatgcta catgggatga
     8461 tccaagccct aatgggggca attctggctt tgtcagtaga aaatatttga tgcttgagac
     8521 cttaactggg gactatggta gccatgaacc agccgatgaa agacccggtc gttatggcag
     8581 tagtcagagc gataaatttt tcgatatttc tgtgcagtga attttttttg atttttaatc
     8641 tttatatggt tattttccac ctgaaccgtc tattttatgg caccgtttga aacaggttca
     8701 agatatttaa aaaagataat tgacggtatc tgttaaaggc aaattaattg ttttagtggc
     8761 tcttaacgct ctattttgta gaatggaagc ggagggagta actgtttagt ttaacatcca
     8821 gcggggcagg caatgagccc gtatgtcggg aaccggcgct tccgtctgta aaaaccgatt
     8881 attcttccgg aggacgtaat gagcaatacg actcaatcac cacaaacatt tgaaacaaaa
     8941 atgcagaata tctctgaata cctaaaaggg cgggttcttg atccggcgga acaggagaaa
     9001 aaaaacattc tcgctgaagc cagggaggag agcagtcgaa ttattgaaga ggctcgcaaa
     9061 gaggccgaga agattgtcgc tgaggccaaa gaacatgccg aacgtgaaaa aaattccctt
     9121 cattcagctt tgcgtattgc ctctaaacag gcggtcgaca cattaaagca ggctctggaa
     9181 aaatcgcttt taaagcagct cgttgccgaa ccggtttccg gtgtgatgag ttcggaagaa
     9241 gtagtaaagt cgtttataac agaagtaata aaaacaacgg acggacagag tgccgagatt
     9301 atgctttctg atgatcttaa gaaaaaaatc tcggattacg ttaaaagtga gataattaca
     9361 aaaagttcgt caaaaatagc cctttcggca gagaatatcc cgtccggctt tgctgtggtt
     9421 ctgtctgaca agcagcttat gctggatttt gccgaggaat cgttagtaga acttctttcg
     9481 ggttttgttc gaagtgaatt gagagaatat ctttttgaaa aatagcaggt gcttatgaaa
     9541 ttgaactact acatagtcgg ttcgctgcct gcattgacct gggacggcga tattcgcgga
     9601 actcttcaag agtatcttaa cgagaatggt tgtatgcttg attcgtttaa tgatgggatt
     9661 gataatattc ttttatacaa tgagattcgt acgcttgaga tgatattgaa caataacgag
     9721 gcggaaatgt ttgatccgtc cattatatct aaagagaggg cgattgcttt tttagaacaa
     9781 cccatgttaa acagaccgga tgaatatccg gactatatcg aagactactt tgtaaggtat
     9841 ggagatacgc cttctcgctt ggaaaatctg aacgaacttt atctagatta tttcactact
     9901 ctatgtgata gtaatatccc tttcttcaat ttttttggaa aaaccgctct gattatacga
     9961 accattgtgt cggcatatcg ccttcaatca aaaaacatct ctttggatga aaaactcgtt
    10021 ggagacgaag aggttgtaca ggctattatt gcccataaaa attcagctga ttttggtctt
    10081 gctcaagttt tcccagagat cggccgactg caatcgatca tggaaaaaaa tactcttgag
    10141 aaagagaaag agttagatct atttcttctt gatctgttat cagagtatag agccgacgat
    10201 ccttttggag atcatgttat ctatttatat atttattcac ttttcttgag agaccgctgg
    10261 gttatgcttg atagcacaac ggcggaaagt ttggtacagg aaataattca ggggtaaaga
    10321 ggagaatcat ccatggcatt agaaaaagct acaggtaaaa ttgttgctgc acagggaaac
    10381 ctgatgacag tagagtttac cggaattata cgacaaaacg agctggttta tgtcaagacc
    10441 ggcggtcaaa gccttaaagg agaagtcata gagattaacg gaaaccttgc caaggttcag
    10501 gtttttgaac ctacaagggg tgttcgttat ggagaccttg tagagtttga tgaggagctt
    10561 ctttcagtag aattgggtcc aggattgcta aagagtatct atgatggtct tcagaatccg
    10621 cttgaggatg ttgccaaaga agccggatat tttcttcctc gcggtatgta tctgacaccg
    10681 cttgatcgag aaaaaaaatg gtcatttaca ccgatggtca aagtgggaga tcccgttgaa
    10741 agagcttcgt atattggttg ggttcctgaa ggtgtaatcg aacataagat aatggttcct
    10801 ttcaggtttc agggaaaggg gaccattgtc gagataaaag atgcaggcga atatacaatt
    10861 gatgatgaga tagcgaagat taaagatgaa aacggaaatg tgcaatccat aaccatggct
    10921 caaaagtggc cttcaaagtt ccctcttcgt tttggtaaaa ggcgttatcc tgatcaacca
    10981 attattacag gatcgcggat cattgacgga cccttcccag ttgctaaagg cggaacattt
    11041 tgtactcccg ggcctttcgg agcaggcaag acagtgttgc agcaccagat ttcaaaaaac
    11101 gcggaggctc aagttgttat tgttgtcgct tgcggagagc gcgctggtga ggtcgttgaa
    11161 atattaagag aatttcctct acttgatgat ccgcaaacag gaaaaaaact tatcgataga
    11221 acatgtatta tttgtaacac atcatcaatg ccggtagcgg ctcgtgaatc ttcagtctat
    11281 gttggcacaa caatcggtga atattataga caaatgggtc tggatacact tcttcttgcg
    11341 gactcgacat cacgatgggc acaggctttg cgcgaaatga gtgggcgcct tgaagagata
    11401 ccgggcgaag aggctttccc agcctacctt tcatcccgaa tagcagagtt ttacgaacgt
    11461 gccggactaa tcgaaaatgt aatgggcgat ataggttctc ttaccatggg tggcgctgtt
    11521 tctcctgcag gcggtaactt tgaagaacct gttacccagt cgacgcttgc ggttgtaggc
    11581 tgttttctag gtctttcaag atcacgctca gatgcgcggc gtttccctgc tattgatcct
    11641 ctaatatcgt ggtccaagta ttttgatcgt ttcggagaaa caggtgacga cgatcttaag
    11701 aagaaagtaa aaatcattca taaggctcag acagttttga aagagtctga cgagatagga
    11761 aaaaaaatgt ccgttgttgg tgaagagggt atttcgctgg aagacattgt tgtttttcta
    11821 aaaggtgaat tctatgattc ggtattttta cagcaaaacg cttttgataa agtagatacc
    11881 acctgcgacg ttaatcgaca gcttgagttg gctgaggttg ttgggactgt aatagacaca
    11941 aaatttaaat ttaacacaag agacgatgca agaaaattct ttgtggatct ttctaaccag
    12001 gtgatacagt ggaactatac cgctaaaggg gatcccgatt ataaagtaca aaaaaatgca
    12061 attttagaac aagtccgggg tgaaaaagaa tgattaaagc atataacaaa atagaaagaa
    12121 ttaaaggttc catcgcccag gttaaagccg aaggtgtgtt tttgggcgag ctggctcagg
    12181 ttaaaatgaa gagtggcggc tcgacacttg cagaggttat ctcttttgat ggggataggg
    12241 taaacctcca gatttatcag ggtacaaaag gagtctcgac tggtgatcag acccgattta
    12301 ttggcaaacc aatgcaggtg cgttttggtg attctctttt gggtcgggtc tttaacggtt
    12361 ctggtgaccc tattgatgaa gggcccgagg tcatggaaga gagtgttgat ataggtggtc
    12421 catcttttaa tccggctcgc agaattattc cgcgtcgaat gattaaaaca aatattccca
    12481 tgatagatat gtttaatact ttggtaatat ctcaaaagat tccaattttt tcgattccgg
    12541 gcgaaccata caatgaactg ttggcgcgaa ttgcaagtca gacagacgct gatattattg
    12601 tactaggcgg tatgggtctt aagtatgacg attatgaatt tttccgccaa tatttcacca
    12661 aatcgggtgc tatggaaaag acatgtatgt ttgtacatat ggcttctgat cctgtagttg
    12721 aatgcctttt ggtacctgat atggctttgg ccgttgctga acagtttgct gtacaggata
    12781 aaaatgtatt ggttctgctt accgacatga catcgttcgc agacgctgga aaggaaatat
    12841 caatttcaat ggagatggtt ccttctaacc gtggttatcc cggatcactc tattcggatc
    12901 ttgcttcacg ttacgaaaaa gctgtcgata ttgacggatc aggttcgatt accttgattg
    12961 cagtaaccac aatgcccggc gatgatgtaa cgcacccagt tcctgacaat accggttata
    13021 ttacagaggg tcagttttat ctgcataatg gagttattga tccatttggt tctctttcaa
    13081 ggttaaaaca gcaggttatg ggtaaggtaa cacgtgaaga tcacggtgac cttgcaaatg
    13141 cgatgattag attatatgct gacgccaaaa aggcaagaga ccgtgaatca atgggtttta
    13201 gattgagtga atgggatcag aaacttatta aattttctgt aatgtttgaa gaaagaatga
    13261 tggaccttgg tgtaaacctc gatatagagg atcagcttaa tcttggctgg gagatacttg
    13321 cctcatgttt tggaaaaaac gaaacaggta tcaagaatga gtggatacaa aagtatggaa
    13381 aatggtcctg acaggagtat ttgatggccg aaattaaatt aaataagaca gagttaaaga
    13441 gtcagcagat gctgatggat cagctttcaa aatatcttcc taccttgcag ctaaaaaagc
    13501 aacagctgca agttgaagta aactctgtta aaaattactt tgatgaattg aatcagaaaa
    13561 tggataaaat gaaaaatgat tttttatctt ggtccatttt gctgtcgcaa agggagccgg
    13621 ttgacattaa agaattttta gctgtcgaag aagttatctc tttgcaggaa aacatcgcag
    13681 gtgtggaggt tccttttttt gataagataa tttttactga tagagaatac tcatcgtttt
    13741 cgacaccgct ctggtttgat aaagccattg aatcattaaa ggaattaatt actgtgcgtg
    13801 aacagatgag aattaccgag atacggcagc agcttttgga agaggaactg cgccagacaa
    13861 acatcaaggt taatcttttt gaaaagcgtc ttattccacg atgtaaaaac aatattaaaa
    13921 aaattaaaat ttttcttggc gatcaggaga ttgcttccat ttgtaatgcc aagattgcaa
    13981 aggacaaact taaacgtaaa gaacttacgg cggaggcggt ttaagtgata caaaggatgc
    14041 agaaggttct ctttgtgggg ccggaatcta aaagagaaaa ttttctaaaa aaactacaaa
    14101 aagccggagt tcttcaaatt gatgactata cgggctcaaa gtttaacaaa tcagataatt
    14161 ttgttgatac taccaaaacc gatcggattg tacagtcttg gaaattatta aagcagtatc
    14221 aaactttggc tgaaaatatt atgcaccaca ataaaaagac agataatatc tctgctgttg
    14281 tggatgatgt gttggcaatc gagcgggagc ttggtgatct ttatgatgag caaaaaagtc
    14341 ttgaaagcaa gattgcaatg attgaaccct tgggtaattt tgatcttgat gttatcagga
    14401 atatagagaa tctttcttct tcgatgatcc agttttggga ggtttctgaa aagaactatt
    14461 cccgtcttga tattccaaaa aaagcttttg taattgatgt tcaaaacaga ggtggccgga
    14521 tttattttat tacaatttac cataaagttc ttcagataga caagacctct gaaattgtga
    14581 taactgaatc cttaaaagaa ctgcaatatc agcttgttaa caacgaacgc aaacagagcg
    14641 aatttaagaa taagcttatt gattttacag tatatcttga cgatattcac ctcttgtatt
    14701 tatcagaatt aaacgagtta aattataaga aggctcttgg tgcaatgatt acccctctgg
    14761 acgggatgct ttttgcgttg cagggatgga catcaaaaga agatcttgat tcgttacgaa
    14821 aattatcaga taataatgaa gcggagctta tagagataga gccggataag ggagaaaaag
    14881 aacccactct tctaaaaaac aaggggctcg gtgaaatggg acaagatctt gttctttttt
    14941 atgatactcc ttcaagtaaa gagtgggatc caagcacatg ggtttttctc tcttttattg
    15001 tattctttgc tatgattatg ggagacggtg gatatggcat tacgcttttt tctttattgc
    15061 tcttctttag aataaagaat cgaaaggcat cggcttcgac taaaagattc ttgaacatgg
    15121 gtatggttct tacttttgct acttttattt atggggcgct gtttgataat atctatgggg
    15181 tcaatttttt tatacccaaa gggctctctt tccctgagtt tatgcttccg gcagtagagt
    15241 ggattaataa cttcaagggg ctcgaaagcg cgcaaaagaa ttctgcactt atgaaaacat
    15301 cagtattgat tggtatggtg catatctcgc tctcgctgat tttgaaagtg ctacgagatt
    15361 tttctaatcg tgtttttatc aaacctcttt cgaacattgc gtggattgct ataatctggg
    15421 ccttctattt ctggtattcg tcgggtgcac agacttcagg tctattacag acgacacctg
    15481 gattggtttt gacagcaggc ttgggaattg tatttttaac aagcgccgga acattgcatc
    15541 ctggcaaact gatagttggt gggcttggag gaatatataa tggagtacag tttttttcag
    15601 atgtactgag ctatatacga attttcgcac tgggattatc gggttcacta atagcggttg
    15661 tttttaatga tttagccgga atggttttta attcgggaat gaatatattt ttagccggat
    15721 tattgggtgc tttgatttta atttttgggc atcttttaaa tatcggactt tgtctaatgg
    15781 gtgctgtgat ccatgggctt cgtttaaatt ttcttgagta ttacagatgg tcttttgatg
    15841 gtgacggacg accgtttaaa ccattttcgg atcttttgtt aagaggagtt gctgctaaaa
    15901 atcagtgatt ctatgtctaa tttttaaata atgagcatta tgttagagta aaaaacaggg
    15961 taattattac tctgaaaaaa taaagagtac gctcttaaat gaataaggag attatatatg
    16021 tctgaattga ttaatgcgca gttcgtagga gcgattttag cgctcggtct tggagcaata
    16081 ggaagttcga taggatgtgg tcttgcgggt atgacatctc acggcgttat ggcaaaagta
    16141 gacgaggggc atggactgat tcttggtctc tctgcaatgc cggcttcaca aacaatttac
    16201 gggtttgtgt tgatgatact tcttcaaggc tcaactgttc aggcttctac tgccttcggt
    16261 attggtcttg gtgctggctt ggcctttatg actagtgcaa tttatcaggg aaaggcttgt
    16321 agctcggcga ttttgggagt ttcgaaaaaa tcttccatta gcggtaaggt gtttcttgcg
    16381 ccaggtattg tagagtcatt tgctcttttt gcgcttgttg gtggtatcgt tctgcttcag
    16441 gggtaataga tcattatagg taatagagag tatttgaaaa attagtcggg gttgtttgta
    16501 ttgaatcagt ataaacctgc cggtttttca atgctctctg agtctatggc aatactcttt
    16561 gctaaagatc tattctttaa gaaagcatgt attattcctt agtaatcttg taagaaaaaa
    16621 atttcaaaaa aaccatcaat tatgtatgaa catggcatga gttattgctt tatccaaagc
    16681 ataaatttct tcacgatgat cggtaagagc tcgatctttt aaaatgttct tcttaaccac
    16741 tgccaatatc tcttttatct gaaaaaggtc tgattttctt gttatttgcc gatcttcgat
    16801 tagctgttga atataaccaa acttgtatcg gcgaaaataa attttcgtaa aaaaatctat
    16861 tccgtaaata ttaagtatgc ggttgagcgg atatttctga gatttgtctg tgaaatctgt
    16921 atattcttca gtatcaacag ctctcatacc ttccggaata atagctgaaa ttgtctgatc
    16981 atcattatcg gcagattcta ttttaacatc tgtttctaac gacttgttct cttcgttaag
    17041 ctcgggaata tccgagtgcc aacgaaatcc tctctcgaca gcgagctcta tcatcttttt
    17101 ttcttgccac tcgtgagaat atgttgtaaa atccttttcg atcatttttt cgtattcgcc
    17161 aagaattatt gtcttgttta gaatttgccc aatttctgtc atattaattg tatgcatttg
    17221 accgaaaagt tctttgatta ttgatgcggc acccatgctc ttgtgatatt gtttttcata
    17281 aagaataatc agctgttttt caaaatagct tttaagattt cgtataacac tcaactctgt
    17341 tactacaaat ccatctatgc ggtcaatgtt tctggctgtg tcctcggcga taatctcgat
    17401 atgaagtatt ttatgcgcgt ccagaagtat ggaaacaact tgtgcgatta tatctttaga
    17461 tacgcctagc tgagccgata gtatattttt aagttcatca gaatgaatta ttgcctgatt
    17521 attgctggca tgattacgag tctctatttg cctaaaaaca gattcgataa tgtcttgaga
    17581 tagtttcatt gattcttccc agtacagtag tgttattaca tatacagtta accaacactc
    17641 ttatacgctt gtcaattcat aatatcacaa ggtggataat gaaacaaaat tttatgttgc
    17701 gttaaattac cggaatattc agagttggca tatttgatat atattggaga atttagaaaa
    17761 tgagtaacga agtgctttgg attgtttttt tgattgttga tcttctattt acattaataa
    17821 catttaagat atttggaaaa aaggggctgt ttgccttaat agctgcaaat attattctat
    17881 gtaatataca ggttgccaaa acaatagagt tgtttggttt tacagttacc ctaggaaata
    17941 ttctttacgg ttcaattttt ctttctacag accttctctc agagtttttt ggaaaaaaag
    18001 aggctcgtca aggagttata atcggatttt tcttcatgtt gtttatgacg gtagtgatgc
    18061 aactagctct tttctttgtt ccaacagaag aaggattggt tgtacatgaa gctttaacag
    18121 tgattttttc tgtaatgccg cgtatagctg ccggatcatt ggctgcatat cttgtttcac
    18181 agctgcatga tgtctgggca tttaattttt ggaaggtaaa aaccggtggt aaaaaactct
    18241 ggttacgtaa taatctatca actttggttt cacaggctat tgattcgctt atcttctgtt
    18301 ttattgcttt ctggggcatg gagcaatcaa tattaattga aataattatt acaacttata
    18361 taatgaaggt ttttgttgca attgccgata ctccttttat atacattgca aaaaaaatat
    18421 gcccctcaga tcagattgcc tgataaagtg gtcatatata aattgataca agcgggtttc
    18481 tttcatgaaa aaaatcagcg caaaattgaa attttgggga gttagaggtt caatacctgt
    18541 accaggaaat gatttcgcgt gttatggagg gaacacctct tgtgtggaac tgcgttgtgg
    18601 tgaaaccatt attgttctgg atgccggaac cggaatacgc cctttaggtt taagttatct
    18661 aaaagagttt ggtgataaac cggttcattt taatattctt atttcgcatt ctcactggga
    18721 tcacctgcag ggctttcctt ttttatcgca tatttatata ccgggtaatt ccgtcacgtt
    18781 ttacggaggg catacggtca atactcttga aaaactggtt atgactcagt tatccgaaga
    18841 gtttcatccc aaacaactat atgaattgtc cgcagatata aaatttgtag aacttaaaaa
    18901 gaataatttt tcgatcaatg atgttcaaat atcttttaca catcttcttc atccagggtt
    18961 atcgttgggt tatcgtatct gttacaaaaa tcatgtgttt gcttatgtaa cagataacga
    19021 aattcttgcg gatgaaaaaa tggcgcatca taactgggaa aacataggtt caattataaa
    19081 aggtgccgat gttgttattc atgacgccca atacactgac gaggagtata aatcaaagct
    19141 gggttggggg cactcttcca tagaaaatgt agtaaagatt tgcaacaaat ttcgcgtaaa
    19201 aaacttatat gcttttcatc atgatccttt gcacacggat aaagatatta aaaaaatgat
    19261 aaaaaacgcc cgcaaattag ctaataaaga tttgaaagtt tatgctgctc gtgaaggcgt
    19321 agagcttctt ttttactaat cattagccat tatcagaaat atcctcggca gatatctctt
    19381 caacttcaaa aaaatctgga actaactctt caaaatgttt gtttgtatcg aaatattttt
    19441 tagtttgcac aagatataat gtaccattgt gcgcaattaa tagataatcg ttaataagtc
    19501 ttttaatgga gtaaagacct ttttctgaaa gctgttttgt aaaaaatgtg tcggttatat
    19561 gaaaatcatc ggcatcgaca tagaaaattg tttcgccgct tattgaaata tccctaagtg
    19621 ttttaaataa tattacctgt tccggtttgt catatgtgaa gaaatgtcgg tatataaccc
    19681 aagcgcctgt tttgccacca atatacaata tacggtcatt cagaagagcg aaaaatatag
    19741 atgttgtttt tttgttttga aacacttttt tgtttgtggt tagtgaatag agagatagat
    19801 tatatgaacc cgcgtttcct atgcagacag caagatggtc tgaacgaatt tttaatgaca
    19861 cgcgaaattg atctttttta aagggtgctg aaaaagtcgt gtcaggtaag agattgttag
    19921 cttctccgct ggagatgtct gctgtaaaga ttctgttgtt gtaaaaataa tacaatgaat
    19981 ctttttcgac gtaaaaagcg tcaatcggct tttcttcatc tttagtaagt tcaatagtat
    20041 taactcgttt gacagtctgt tcctgggtct cttcgtttct tccaaataca aaaaactcgg
    20101 ccttgtatcc gtcggaagta cataaaactt ttgtttcagg attatacgaa gcatgaagaa
    20161 tatcttcgga ccagggttct ttaaaaccat cagaagtaat tatggtagtc aaggactctt
    20221 tttcaagaat aatgcagtta tgccccgcaa tatagttatc agctttatct atttgaacgg
    20281 cacctagtga attgaaatag ttttcttcaa tgacaagtct ggcttcaggg tcttttgatt
    20341 cgttatattt taaaacagct tcctggaatt tttcttcggg cgttttggaa caggaaaaac
    20401 aaataagaag tagaagaggt agagcttttt tcatgtttca gatcctttta ctttggcctt
    20461 gcctttaatt ttttcaattg attttgacag aaaatctttg gcatatagtt caattgattt
    20521 tgcggtcgcc gctgattttg cctttaaacc gtatcttgac gtggttatgt atatgccaga
    20581 tttacatttt tcatcacgca aaagccgcga aaaaaagtta atatcgtttt ctgaaatttc
    20641 gtgagttgat ctggcaaaaa atattaatat tggtggctca ttaattcttt tagtgtgata
    20701 caccttgtaa taggcctgat tcttattcaa gaatgttttg ctaatgatat tatagttcaa
    20761 acgtgctata atttctccca aaagcggctg aagcgcgtca aaagaagaag tgaaaatttg
    20821 tttcatataa acatcttcta gcaacgcctt gtattcatca agtttaatct gggtgctacg
    20881 gtagttgggt tgttcggact ggattttttc ccagtgatga acagcctctt gtactttgtt
    20941 atccatctcg taagattcag ccaaaagata acgaaattca atcgattctt cgtctcgtgg
    21001 cttgagggag cttaaaccag tttcaagggt tgttatggcc tggtcatagt ttgctgtttc
    21061 aaaataaatt tctccaatgc gcataagact ttttcgaaac agtttgctgt ctgttttgga
    21121 tctttcaaac gagattatgg cattatcgta gtctttttta cctttcagaa tcagccccaa
    21181 gtagtagtgg gcttcctctt gcttgtaggg ccgttcgatt gcttttaaaa gaaaattttc
    21241 ggcattatta taatctgcta tctggtaaca tgaaattcct aaaggtaaat aacaatcatc
    21301 aagcgaaggg tctgagttga tagcacgcat caaagcatcc ttggcatctt tataacggtt
    21361 ttgtttataa tagcttaaac caagcctgtg attagcatta atatcgtctg gattgaatgt
    21421 cagtataatt ttgtattcat caatttcttt tttccaggcc tttgtactgt tataaagttc
    21481 tgctaattcg taatgaattt taagctcgtt gacatgacgg ctgaagtcgg gtgtttgaag
    21541 aatagcatta aattcggaaa ttgccagtac aaactgtcct tgttgatgaa gcatttgtgc
    21601 ccgtagataa cgtgctggaa catagtcctt ttttttgtta agaacagtct taattatttc
    21661 gctggcttcc ttgtcgttgc cgtttttaat catctcctga gcccttctga cacgggctgg
    21721 cagtctcatg gttttgtaat aatagacaaa agaggagatc agagagacta ttataaaaaa
    21781 aagggttatg ttgactatat tcatatttct ctctaattgc tgaaaatatc aaaatcatcc
    21841 aatgaatcaa gattatctat gaaatcaata gattcagttg tatcaaggac ggaatcatta
    21901 atagtatcgg ctggaacgtc cgtggattta agggaaagtg ccttgtcagg gtcaacaatg
    21961 tcaatttcct ctcttttaga ctctttccat ctctcatata tttgcaaaaa gtcatatttc
    22021 attttttttc tggtaatatt atcaagattc atcttttcga agaaatagac cattgtgtcc
    22081 atatagaaag aatcacgttc ctgttcttca agaagtttta cctctttaag ggttgtcttg
    22141 tttttttcac agatagcttc tatactcata cgaatcattc cgcgaatgat tgatattgat
    22201 ttagaaacct ttgtcgatgt ataagttgcc agggctctga ttaaaacttc ttggtcagga
    22261 tagccaaaat tgttggacat tattttattc cttggttgac tgtgaaaagt gcctgcgtta
    22321 ttcatacaat ataattttgt tataaaaaaa atcagtaaag aattaggagc tattcaaaaa
    22381 aaagacaatt tcatagtaaa gtggtaaata aatagttcaa gaaaaaaaaa cgaaaattgc
    22441 aatctgttca aaaaatgata agatagccga tggtaaaggc tgggttttta tagcgaatct
    22501 gatattaacg aagagaaaca aagcccgcaa aggcggaagg attgatcagt tctgagcttt
    22561 tctttaaaaa atggataaaa acagacagcc tagataaagt caaataaaag ccttgatgat
    22621 tgtattagat gctggctcca atggagatgg acgcagccca accattgcca ggataaaata
    22681 cagctggtat ttcaattctt ccagggccaa attcgaaacc taatccgaat atcattttat
    22741 atcttagata agagccatga tcatctgttt tgctatcggt taaattaagt tttgccggat
    22801 tgtctgaggt aatcggatta ccctgatcat caaaagagct tgtttttata tctgcgtcag
    22861 catttaatgt agagctgcca agatttaggt ctacaccaaa accggcaata atgttcagaa
    22921 agtggaaagc atttgctgtt gtgaccagat caatgggggt tactacattg taagtgtcca
    22981 tttgaaatga aacatcagtg gatgattcgg ttgtgattcc gttgtccgca gttatttcct
    23041 tttctaatgt atcggaaaaa aacgagagag ttgcgctgct gtagtagatg cctgttccgg
    23101 cagttagaga acgtaactga aaaaacggat tttgtacggg tatttcaaaa agtttatatc
    23161 gtgctccaac accaaacaga aaagaatcaa atttaacatc agttataaga gaatctgcct
    23221 taaagtaacc tatttttgtg ttaaagacaa taccttttat tttaataaaa ggtaaattgt
    23281 cggcgttgac attgcaatcg acagtaaagg cgcttacagc aaagctcgat tttttatcgt
    23341 aaccttgacg tatgttgtta ggaaattctt ccaaaacaaa agggttttca gcaccagccg
    23401 cgaatccaat actgacagag cctaatcggc tgtccattgc tgaaaagagc gatccattaa
    23461 ggaaaagacc gcctgccgcg ttatgtgttg cctgggcaag attattttgc tttcctgata
    23521 gcatgataat tgaatcttca tttatatctt ctgcaatttc actcatctgc tcgttaatgg
    23581 tgtctgtaat ttcgggggta gcataagagc cttccgttac ggtgacagtt ccagattgta
    23641 cagaaaaaac tggtaaaagt agtatgataa tagataaata aatttttctc attgaactac
    23701 tccggctaat aaaaagttat tatctgaaaa ttaatattat tttctttaat ctatctttaa
    23761 acgacatgac tgttacgtaa atgataattt ttagaagatc tcttaacaaa tatattattc
    23821 attcacaata aaaaatagta taaaattatc tattataaaa aaaaagcctg aaataacagg
    23881 cttttttatt taaaggagca cagaaagtaa aaacaagcca aattgcattt tgagcatgcc
    23941 atatctgtaa aatatagcaa ttaaactgta ttgataagaa taccgtcacc tttcgaactt
    24001 atttttactg ttgatcccgg taatatttct cttgaaagta ttttaagaga aagcggatta
    24061 agaagctctc tttgaattgt acgttttaat ggacgcgctc caaattgggg gtcccatcct
    24121 ttttttgcca gaatcgcaag tgcgtctttt gatatttcaa gttcaatatt ctgttgtttt
    24181 ttaagccgct gtgcaagcat taccagttga agctttacta tgcccgctat atgatccttg
    24241 cttaaaggat taaatattat tgtttcgtca acccgattaa taaattcggg tttaaagtgc
    24301 attttaagtt ctcggtgaac attttcgttt cttgtttcta tgggtatcga ttcatcgcta
    24361 atatatgtcg acccaatgtt tgatgtcata ataatgattg tattgcgaaa atcaatggtt
    24421 ctacctttgg agtcagttaa tctgccatca tcaagaattt gcaaaaatat gttgaaaaca
    24481 tctggatgtg ctttttctat ctcatcaaag agaattacag aatatgggtg acgccgaaca
    24541 gattcggtta actgacctcc ttcatcatat ccgacatatc ctggaggggc tccgattaat
    24601 cgtgctactg catgtttttc catgtattcg ctcatatcta tacgaactat ggctctttcg
    24661 tcattaaata aaaactcggc tacggctttt gctgtttcag ttttacccac acctgttgga
    24721 cctagaaaaa taaacgtgcc aactggttta tcaggatctt gtatgccgga tcttgagcgt
    24781 cttacagaat cagaaatggc tgtaattgcc tggtgttggc cgaccaccct cttttccagc
    24841 aaggattcca ttttaagaag tttctgggtt tcgccttcaa gcatttttga caccggaatt
    24901 cctgtccagc gtgctacaac agaggctatt tcttcttcgc ctacctcttc ttttagcaat
    24961 tttttttgcg actgtatttc agataatgct agttgctgta cctgaagata tttttccagt
    25021 tctacaattt ttccgtacct gatttctgct acacgattaa ggtctccggc tctttcggcc
    25081 tgttgttctt cgttgcgcag atgttctatt tcttcttttg ctcgacgcac acctgttatt
    25141 atttcttttt cattttgcca ttgagccata agtgtgcttt tcttctcatt aagattagcc
    25201 agttcctgtt caattttatc aagatgttct tttgatgtgg catctttctc ttttttgact
    25261 gcttcacgtt cgataattag ctggcggctt cttctttcaa tttcatcaat ttcaataggc
    25321 attgagtcaa tttccatttt tatcaatgaa gctgattcgt ctataagatc cacagcctta
    25381 tctggtaaaa accggttagt tatatagcgg tcagaaagaa tggcagcagc aacaacggcc
    25441 gcatcagaga tgcgtactcc atggtgtacc tcatatcttt cttttagtcc gcgtaaaatt
    25501 gctattgtgt cttctacaga aggctccgat gtcattacag gttgaaatcg tctctcaaga
    25561 gccttatcct tttcgatata tttctgatat tcatccagtg ttgttgcgcc aatacactgt
    25621 agctctcctc ttgctagcgc aggtttaagc atatttgacg catcaacaga accttcagaa
    25681 gcgcctgctc caaccagagt atgaagctca tcaataaaca gaactacttc gccggccgaa
    25741 ccttcgacct cttcaataac agattttaac cgttcttcaa attcaccgcg gtatttggcg
    25801 ccagcaataa gagctcccat gtcaagcgac acaaccctct tgtctcgcaa gttttcaggt
    25861 atgtcgccgc ttacaatgcg ttgggcaagg ccttcaatga ttgccgtttt tccaaccccc
    25921 ggttcgccta ttaaaacagg attgttttta gttcgtcttg tgagcacttg cataactcgg
    25981 cgtatttcgt cgtcacgacc gattacggga tcaagtttgt tttgtctggc aagtcgtgtc
    26041 aaatcacggg caaatttttc taaagcgtta tatttgtctt ccgcattctc gtcattaacc
    26101 gatctgtttc cacgtatttc agatagtact cgaagtacgt tttcatagtt aaatccgtta
    26161 gcgtgcaata tcttatatga agctgtattt ttaaccaggg taagagcaag aaccaggtgt
    26221 tcgctgctca aaaacttgtc ctgaagtttt tgtgcttcat tccaagctgt attcatgatt
    26281 tccctgaatg tttgtgacag agatccacca cctgggcctg ctccagtaac acgaggaagt
    26341 ttttttactg cttcggtgca ctcagttgct atcaagtcga caggaacgcc aattttttga
    26401 cacatggatg tgaaaatcga tccatcttgc tgtgttaaag cagtaagcaa gtgaattggt
    26461 tgaacttcgg gatgatccga tttggttgca ttttcttgag caagagctat cagctcctga
    26521 gcctttatgg ttagtttgtc cattctcata attaagacct ccaatttagc actcaacata
    26581 taagagtgct aacaaaatat acaaacatgg tttgatttcg tcaatgcttt tttgtgtaaa
    26641 agcggaagta gataataatg attcttacga aatagtgttt gaattccgat agatatagta
    26701 tagattcttc attcaattaa catatagcct taaagaattt cttaaaccat gatgtatcct
    26761 taaaaataat gagaattaag gtttttacaa tgataaatcc tgacagtcgt tttaagatta
    26821 tctgggaagt atatatactt ctgcttacta tagttgttgc cgttcaagca ccattgatga
    26881 ttgtatttga gattaatcca aaaggaatat tgttcttttt tgatatgata atcagtttat
    26941 cgtttatggt tgatatagtg cttaatttta ataccaagat aattgaaaaa cgcagggtta
    27001 tagaagaccg aaaaggggta acaaaagcat accttaaagg ttggttttgg attgattttc
    27061 ttgcgacaat tcccttcgat attttttacg taacctttca acttggatcg ttatcaagaa
    27121 ttttgagatt attcagattg gcgcgccttt ttaggctttt acgattgatt cgaatagcgc
    27181 aaacaatgaa acgcgttaac aattccaatc tgtttaaccc aagtgtatta agacttgtgt
    27241 ttatggtctt ttggattttt tttgcggcac actttatcgc atcagcttgg attgttatat
    27301 tgggaaaccc aaataatctg gataatgtaa ctcgatatat tagatctgtt tactggactg
    27361 ttaccacctt aaccacgatt gggtatggag atataacacc ggttacaaat gcccaaacga
    27421 tgtttgtaat tataatcgag attatgggtg ccggtatgta cggttttata attggtaata
    27481 tcgcgaacct gattgccaat attgatattg ccaaggctca gtataaggag aaactcgaga
    27541 agattaatac ctttatgaag tatcgcaacc ttccttcacc tttgcaaaat cggataaatg
    27601 agtattacaa ctatctttgg gagagcagac gtggatacga tgaaagttca gttcttctgg
    27661 atttaccaat ttcattaaag actcaggttg cactgtatat taataaggat attatagcaa
    27721 aggtgccaat ttttaaaggc gcagaggaag catttatcaa ggaagtaatt ttgaatcttg
    27781 agccagttgt gtttacgcca ggagatacaa ttgtttacaa aggtgacatt ggttttgata
    27841 tgttttttat cagcaaggga tctgtagatg ttgtttcaga ggatgaatcc attgtttacg
    27901 cgaccttaac taccggacaa ttttttgggg aaattgctct tttattttct tctccgcgaa
    27961 ctgcaactat aaaggctcga gaatattgtg atatgtatag attaaaaaaa gagatctttg
    28021 atgatattct tgagcgatat cctgattttg aaaagcagat caaggagttg gcggacaagc
    28081 gaaaggccga aattatggcg gaacagatag gaattaataa aaatattaaa acggaagtag
    28141 tagattctcc ggatactgtt atgaatttac gggctgaaag gtcagaaaat ggcattacgc
    28201 tttcttggaa tggaccacac ggagaaattc cctgccatta cgaaataatt cgtaaacagt
    28261 ctgataatcg ttggaagatt cttgactctt cggttaaggt tgaaattttt tctgataaag
    28321 atgctgatcc agacgaagtg aatgtttata aggttagagc tgtaaacgta ggtggacctg
    28381 gtccatggag caattcggtt aaaatatgat tctgatacag taactaatag tcacaaagtt
    28441 gaataaaatt atgacaacct ctactgatta actttataac aaggttgcgc gagcagtcca
    28501 gcaccatttt ctttataaaa atggtactgg acgatcagca aaggagcttg tgaaaaattc
    28561 ctttagaata gacgcttaaa ccatgtcgcc ctctggaaca ttgtaatctt tcgaagttcc
    28621 cttattagta aaatgcttta ttaaaaaagt gatttctgta tcataattcc attttgtttt
    28681 aaaaattctt ctgttttact gcaaacaggt gccgttgtgc gtagagagac tgttgtataa
    28741 ggaagctttt tgcgtatttt atcggctatt tgtaaaattt tttccccgtc tttcattatg
    28801 attctacttt taacaatctt tactatagct atatgatcgg acgtaacttg ttgtagaact
    28861 gttgaaggtg gaagttcaaa atcacgggca tcaagatttt gcatgggggc tcctgttttt
    28921 tgagataatt gaaatttttt gcttgtgtcg atctaacaag aaataaatca ttatatgaat
    28981 ctttaaaatt tcaataaaaa taaagcgatc ttgcgattat ttagcaagtc atgttttgtt
    29041 ggtttcatgc ttaatgataa gaatttagct agactgttgt tcataataaa cagtatgttt
    29101 gctattatgt acgtgtgccg gagaactaat tcatgaaaaa actagtagcc ctattgttgt
    29161 tctttgtgtt taccataaat ctttattcgt caagtgaact tgattatgac atttatctgg
    29221 gtttcgtaaa aaacaatttg cccgaattga ttaaaacccg gttaaatgaa gagattgcaa
    29281 gacgtgaagt tcaaaaagca agtggtataa acgatacaaa aattaatggt tcgttatact
    29341 attataaaaa tgacccaaca caggtacaac aatcacaggg ggtcgaagat actactgggc
    29401 taactggaga ggctgaagta acaaaaaaaa ttcttcccag tggaacggaa cttttcggag
    29461 gagccgcatt atcgagatca gttgttgaca gagatccgca acagactgct tatcagcctt
    29521 ctttaacagt tggagtcaga caacccctgc tttataattt tttgggaagt gtagacagat
    29581 tttcggaaca aaacgccaga cttggatatg aaattgaacg tatacagacg aaaattacag
    29641 aaaattcttt gcttaacagc tatgctctgt tatatcatca atggattgta tattcgcgaa
    29701 ttgccgaaat aaatagaaaa ggcgtagtta atgcgcgcac tgtctataat cagatgttgc
    29761 gtaaaaagag aagcggattg gccgaaaacg acgaggtaca aaaatcgtat tcatctctac
    29821 tttcgtttag acaaaaacat gagcaaagca tgattgatga aatacaagtc cgagaaaaaa
    29881 ttgctgtatt aacaggtctt gattccatca agcctgaatc gagtcaattt gacaggcaat
    29941 attctctagt gaaagagttt tcttttgatt tacgaaattt caacacaaca acatcgtcaa
    30001 aatacattga tcttctggcc gaaagaatga agttgagtcg tgaaaccgca gaaaataaac
    30061 ttatgcctca agttgatgca gtcgcttctg ttacgaaatg ggatgaaagt gactcttttt
    30121 cagattcggt ctcttatggt aaaacggatt acaaaatcgg ggtagagcta tcccactctt
    30181 ttggaaataa tagcgctaaa gcagagtacg aaatatccaa attggaaatt gtcaaaattc
    30241 aggaagatat gcgatccgcg ataaactcat ttaatacaga aataaggcag atgcgcgacg
    30301 aatctgaaag aattaaaact ataataatct ataaagagag aaatattagg gcattgcggt
    30361 cacagttggc taccgaaaca cagaaatatc agtcggccag aatagatctg gcatatctga
    30421 ttaatactag aaacagcatt gctgccgaag agagctctct gcttttgcaa aaatataatt
    30481 taatatctct ctattatgcg tattctgaac tggttgaata aaaaatgctt aaaatgaaat
    30541 aatgaaatgt gaaaaatttt gtcaggtctg ttcttttttg ctatattgta aatgaggtat
    30601 atatatggcc ggaaacaaaa gcagatcttt acgaaatatg aaaattgtga gtcttattga
    30661 ttatcgccaa aaaaagttaa aggatcaata tgatcaagac tgtcttgatg aagaaaattt
    30721 tatcatggaa tctatttttg aactagctcg tgattatgat gatgagcggg aatggcgaaa
    30781 taattatatt ctgaatatta ttaattctga cgagtttgtt gatgattatt tttttggtag
    30841 ggagtggaac gagaacgata gacttgttaa gtatttactg atgatgatgc ccggttataa
    30901 cccagaaaaa ctatatactt caaacgatat tgatcttcta atggttgagg ggaaattccc
    30961 tgttgattaa ataaagctac tgctattaga taccctttta aaaaattaaa ccttaaaatt
    31021 tttatctctt ttcaatggag ttctgatgaa tccttccaag atagtttgtg tcggtcttaa
    31081 ttataagcgt cacgcagcag aattatcgct gtctgttccc gaagagccgg ttattttttt
    31141 aaaacctcca agttccatga taggatctgg cgatttgata cattatcctg ataaagtaac
    31201 aagagttgat tatgaggcag aactgggagt tgttattcat aaaacttgtc gtaatgtgga
    31261 caaggattcg gctcttagat atatttatgg ttactgttgt gttaatgatg tcacggcacg
    31321 tgatattcaa accaaagagg ttcagtggac cagagcaaaa tcgtacgata ctttctgtcc
    31381 ggttggcact atcgtaaaaa aagagaatgt cgatccgtca aacttaaaga tatgctctta
    31441 tgttaatgac gagataaagc agaattcgaa tacctctgat atgattttta cggttgcaga
    31501 gtgtatatcc tttatttcaa gcataatgac actgttgccg ggggatctga ttcttaccgg
    31561 tactccggaa ggtattggac ctttaaacag gggcgatcag gtacgtgtcg agatcgaagg
    31621 tttcccggtt cttataaata gtgttagttg aaaaggatat agatggtttt ttcgaagatt
    31681 ttgattgtat ttatgtttac aaatgattat atggaataac tatggtgtaa ataattgttt
    31741 taggaacaac gtccgaggaa tataccgcta ttatcctgcc cctggtttta tcagattcat
    31801 tattatcaga acttttcacc agtagatcat atatcagcag aatagaacag cagtgctttt
    31861 tttaggtaaa caatgcaaat gttccaaaaa aaatggacaa tttggagaaa taatgatgac
    31921 ttttgacaca atggggcttg gccctctgat tttggatgct attgataatc tgggttttgc
    31981 cacacctacg cctgttcagg agaaagtaat accatttgta ttgcaaaacc ggcgtgattt
    32041 aacagcactt gctcaaaccg gtacaggcaa aacagcggca tttggtttgc ctatacttga
    32101 acaggttagt gaaaataaaa atgtgcaggc gctgattctt gccccgacta gagaattatg
    32161 tattcaaata tcaaaagata tgctctcttt tgcaagattc aaaccagaga taaatatcgt
    32221 acctgtttat ggtggagaga aaatagagtt acagattaat aaactaaaaa ggggtgctca
    32281 aatcgttgtt ggaacacctg gtcgtcttaa cgacatgctt cgccgtaaat atttggatgt
    32341 ctcttcaatt aagtggctcg ttctggacga ggccgacgag atgcttacta tgggttttcg
    32401 ggaagagctg gaatcgattc taatgacaac tcctgataca aaacagactc ttctcttttc
    32461 ggcaacaatg cccgattctt taaaagacaa ctttatgaaa aacccggaag agattgttat
    32521 tggtaagaga aatgccggta acacaaatat tgaacatcga gtttatatgg ttaagtcacc
    32581 agatcgttat ttggctttaa aaagggttgt cgataatgct cctgatattt acggaatagt
    32641 tttttgccgc acaagaaacg agacaaaaga agtttctgac aagctgatta aagatggata
    32701 cagctgtgac gcacttcatg gtgaattaac gcaggctcaa cgcgatcatg ttatgaagag
    32761 attcagagaa aagcatttac gtcttttgat cgctaccgat gttgccgcaa gaggattgga
    32821 tgtaaatgat ctgacacacg ttatcaatta caatttgccg gatgatgttg aggtttatac
    32881 tcacagatca ggacgaacag gaagagccgg caaaactgga atctctgctg taattgttaa
    32941 tacacgagaa cagagaaaaa tagaacagat tgaacggact atgaaaatac attttgagag
    33001 ggcacttgtg cccggaggcc gcgaaatttg tgaaacaagg ctttttgatc ttatcagccg
    33061 ggtcgaagag gttaaactta aagaagaaca gattaatagt tttatagagc ccattattga
    33121 aaaattgtca gtttttgaaa gagatgaatt gattaaacgt tttgtttctg ttgagtttaa
    33181 tacttttctt gaatactata aaaatgctaa agatataaat gccaaaagta caggaaattc
    33241 aagagacagg gggtctggtt t
//
LOCUS       DAOTGZ010000007        32703 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_3064
            NODE_1513_length_32703_cov_8.384985, whole genome shotgun sequence.
ACCESSION   DAOTGZ010000007 DAOTGZ010000000
VERSION     DAOTGZ010000007.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868088
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 32703)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 32703)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:22:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,785
            CDSs (total)                      :: 2,751
            Genes (coding)                    :: 2,732
            CDSs (with protein)               :: 2,732
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 12 of 19
            Pseudo Genes (internal stop)      :: 5 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..32703
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1513_length_32703_cov_8.384985"
                     /isolate="AMR_MDS_3064"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(118..1704)
                     /locus_tag="P5123_01125"
     CDS             complement(118..1704)
                     /locus_tag="P5123_01125"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRX14895.1"
                     /translation="MKIVLSVFIIFFISFQSFSNGAEIIYKNSAEFRKAEFKHIISGK
                     APSGSCNAITRREFSSRLSGDTVNENNGFNTSMDFTLTPYAAAFFSPYATYGKTYPLQ
                     MVYLHDKSIDALKIKADIEYEESLYLKFTYSLSSSAKSISDGWNAHHPLNPDFYSPAD
                     SPDEGYISYAKNHFSLTIGRFRGGIGHGLIGNTFQNGKAPYYDQIQLNLYSTKFKFYF
                     MLGSSNHQLSKEEDFIQDRRSDYTDYEKTWGTAYNNRHKQSKISDNSKMFAFHRVEVS
                     PFDKFSFGIGEMNLIGGKFPDLNQINPLGIYHDTYDPDFHSYTFSIDASFVPSPNHFL
                     FSELLINEIEVTGERNEDPTALGAQIGYWYILPTKSSSFHRIAFELTHIDTWTYSDNV
                     PYLTMYQRQYRNQFNYDIPLGYSYGGDCEQFSILYTYISGNNTMIDVTLSHLSKGEIN
                     FSPLEDGTMPYEYADKWQARPSGTVEQWSSIDVTSSIFLDSTKSIETTAMYSYIQNFN
                     HKKDNTNHLLYISAGLSWHF"
     gene            complement(1701..2021)
                     /locus_tag="P5123_01130"
     CDS             complement(1701..2021)
                     /locus_tag="P5123_01130"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRX14896.1"
                     /translation="MSVSSEEMIEVEAHTAAFFIFSPPKEAIYTLSVYNMSTDFDLTL
                     GIYCDTELFSDQIVIAVSKNLYLEDETIEISLSPDEKYLIEIWNTYEDTGSCRFNITE
                     KVAP"
     gene            complement(2222..2812)
                     /locus_tag="P5123_01135"
     CDS             complement(2222..2812)
                     /locus_tag="P5123_01135"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRX14897.1"
                     /translation="MLINKYLLISFLTLFISLNLTGQTEQPADSSEKASASWGVKLMK
                     AEFENEDFYIGCVPFFESATNVFFNGMYLYSNSNLTWGLTDFFFDFGLGAGLRYYPLN
                     NKILSIYGGGELSTFFFNNATTTGRSGADLDFIINNESSVFLGGEIFYRKVYRVAEFI
                     ESDHWYITSRGWSVNGGFRARFSIFKDGIKKMPNPI"
     gene            complement(2857..3252)
                     /locus_tag="P5123_01140"
     CDS             complement(2857..3252)
                     /locus_tag="P5123_01140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013297070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="secondary thiamine-phosphate synthase enzyme
                     YjbQ"
                     /protein_id="HRX14898.1"
                     /translation="MTTIQLKTNSHDQLIDITADVRKVIKDSDIKNGICIVYTPHTTA
                     AITINENADPDVITDMVTGLNSLGFEKLPFRHMEGNSPSHLKSSLIGCSEQIIVENGT
                     PVLSTWQGIFFCEFDGPRSRKVFITLTGK"
     gene            complement(3260..4519)
                     /locus_tag="P5123_01145"
     CDS             complement(3260..4519)
                     /locus_tag="P5123_01145"
                     /EC_number="2.7.2.4"
                     /inference="COORDINATES: protein motif:HMM:TIGR00657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004072 - aspartate kinase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate kinase"
                     /protein_id="HRX14899.1"
                     /translation="MKIFKFGGASVKNAESFSSVTNILKNQTTKHDTLVIVISAMGKT
                     TNLLEKLTDAYFEHNIEEVKNLYKQTRDFHYLQSIKLFGDTSLKHIEIMDILYKLNER
                     LQENVSDNYDYEYDQIVSYGELLSSRFLSSFLEKNGFSNTLLDSRKIIITDSSYRSAS
                     IKWDITKTNIDLKVKAHPGHIYVTQGFIGGTEDKTTTTLGREGSDFSASIFAFCTNAK
                     EVTVWKDVPGILNADPLFFPDAVKIDFLSYHDAIELAYYGAKVIHPKTVKPLQNKGIP
                     LYVKSFIQPSGSGTKISEISSHTKVPSFIIKKQLILLTISPKDFSFVTEKSLSFIFAL
                     IADNSLKINMMQNSALSFSLCLDTNSRIKNIIQTLSIDFSVKYNENIELITIWHYNDN
                     TIRTLLKNKTVLLEERTRHTVQFLVKG"
     gene            4818..5714
                     /locus_tag="P5123_01150"
     CDS             4818..5714
                     /locus_tag="P5123_01150"
                     /inference="COORDINATES: protein motif:HMM:NF014069.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HRX14900.1"
                     /translation="MNISEALKKSGLSVIESLFSDLHSGKDLDMESAEKLIDSLIQEI
                     SHQKEGMLYLPDPEKTSEYLIYHTLHVLMLSLIMGYRTGIEGMELRALGLGAVLHDLG
                     KINTPENLVWKQDGDNEYEKTVLSEHPEMVIHWLTQKNIISDEILVIIKGHHERYDGL
                     GYPSGVSTRDFNQSSNIVSICNYYSFLTINITEKPGEEPRNAFFTISKEMGKRFSPKI
                     INQFISVMGPMLMDGPIYRKTALVLLDTKEVAAVVKTNGFADTLPEIIVLTNCQGKKL
                     ERPVSVDLKKDGSRRIIKLLKA"
     gene            5718..7241
                     /locus_tag="P5123_01155"
     CDS             5718..7241
                     /locus_tag="P5123_01155"
                     /inference="COORDINATES: protein motif:HMM:NF025885.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008134 - transcription factor binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HRX14901.1"
                     /translation="MRQNIDYLYAVGDIFTSGNNLDDLLKEAIELTLKKLSIIRLMVH
                     IYQDDIEEIVVDIYSGYSADEIKRGVYKKGEGIVGKVISDGKPVIIPRIGSCPEFLNK
                     TGSRSTQADLDGAFVCVPVRLKDKTIGTISADFSDVEEDFLSYAAEQLAVIAILSAHE
                     ISSRIEKQKSERQLVEENRRLNARLSEKIKNIKIIGNSVLMHELFEKIALVSETNTTV
                     LITGESGTGKELVADAIQKNSLRSDKPFIKVNVAALPENLIESELFGHEKGSFTGAVQ
                     TKKGRFELANGGTIFLDEIGDLALPLQVKLLRVLQERFVERVGGVETIPIDVRVIAAT
                     HQNLEKKIELNEFRSDLYYRLNVFPVNVPALRERKADILALVNHFCEKFSKETQKSIN
                     RVSSEAIDMLVAYHWPGNIRELENCINRAVILAEEGVIRSYHLPPTLQIAKNVSGSGS
                     FENMVNNFEKEIIIDYLKITEGNISKAAEMLGTTKRILTYKVKKLEIDCDRFKKIRS"
     gene            7280..9691
                     /gene="lon"
                     /locus_tag="P5123_01160"
     CDS             7280..9691
                     /gene="lon"
                     /locus_tag="P5123_01160"
                     /EC_number="3.4.21.53"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008728303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endopeptidase La"
                     /protein_id="HRX14902.1"
                     /translation="MSKDNKNNEEITIPSESLENDLVSIDHILPKQIHIIPIRYRPIF
                     PGIITPLIISKGRFSDAIDQVLDNSRSIGLVLLRDDEVDDIKPQNIFQIGTGAKILKK
                     IKLPDGGINVLINSLKRFRIVKVIQEKPYLIAEVEYLDDKLPPKSRRNELKAFTRSVL
                     SQLKQLSENNPLFTEEMKLTMVNVDEPGKIGDFVSSILNLEKEEYQTILETLDVVERL
                     RLVLHMLQKELEVIEIQKKIQNQINDKIDKQQREYFLREQLKVIKQELGLDADERSRE
                     ISEMEAIISELDVEEEVLEKLTEEIEKLKNMEPNSSEYTVTRNYLDILLALPWNEKSE
                     DNLDLDRSEKILNKDHFGLDDVKERILEFLAVRKLKPDASGSIVCLVGPPGVGKTSLG
                     KSIARALNRKFFRMSLGGMRDEAEIKGHRRTYVGAMPGKIMQGIKICKTKNPVFMLDE
                     IDKLGQSYQGDPASALLEVLDPEQNSTFRDHYLDVPFDLSDVFFLTTANSLDTIPRVL
                     LDRMEVIRLSGYISEEKYEIAKRYLLPKQLEKHGLLKNDVKIDKNGFMFLIQSYAREA
                     GVRNLERVVEKICRKTASIKARKNKLPGDNLSYEQIREYLGSERFVDDEKFEIDRPGL
                     INGLAWTSMGGAILVVEAISIKQTKNAGLKLTGQLGDVMSESANIAYSFIRQYLSEDE
                     EMAKLFDSSLIHLHVPAGATPKDGPSAGITMASAIYSLVTKRIAKKSFAMTGELTLSG
                     KVFPIGGLKEKLIAAKRAKIKNIIYPSLNEKNLEEVPDNVKKGLKFYPVSEISEVLEI
                     LFPKK"
     gene            9704..10375
                     /locus_tag="P5123_01165"
     CDS             9704..10375
                     /locus_tag="P5123_01165"
                     /inference="COORDINATES: protein motif:HMM:NF022179.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="late competence development ComFB family
                     protein"
                     /protein_id="HRX14903.1"
                     /translation="MNIRNMMEGIVSSLVLEVLKKEERLNESKEYFEDICAYVLNRLP
                     AKYVTSDRGILHGILETKFSAQQKSDIIMLVYEAMTIIHERRDKNHKHIHISKGAPNF
                     FFPHLLGEVLEKTTFSKIPGVKVTLLKDKSPVKMVSDGWDNPFHTNNVTGSYYHFWPL
                     FDEESDPLLEQKFLVTFEHEKFKPFEQDIYVRPVSLTSGAIETRIVAVVLLDLKEGED
                     LSFLE"
     gene            complement(10387..10851)
                     /gene="smpB"
                     /locus_tag="P5123_01170"
     CDS             complement(10387..10851)
                     /gene="smpB"
                     /locus_tag="P5123_01170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013276571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SsrA-binding protein SmpB"
                     /protein_id="HRX14904.1"
                     /translation="MSQQKEFDIAKNKKARFQYDIIETFEAGIVLKGTEVKSIRNGKV
                     NISESYGRIRDGEVYIMQMNISPYEQGNRFNHDPAQPRKLLLHKREISKLYGKIMEKG
                     LTLVPLKLYFKNGKAKIELALAKGKTLYDKRHSIKQKQDKREMERAIKEKNR"
     gene            11281..13662
                     /locus_tag="P5123_01175"
     CDS             11281..13662
                     /locus_tag="P5123_01175"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HRX14905.1"
                     /translation="MKNILIVEDEKIIALDIKNAVQKYGYNVIGSVTSGEDAVEFCKR
                     SLPDIILMDIHLDGEMDGIDAALAIKENLEIPVIFLTVSVDSVTLERAKKVEPYGYIN
                     KPVKDVEIFTTLEMALHKFRTELKLRQNEQWLSAMLNGIGEGIISVDIDGVITFMNRI
                     AEKMTGWIASDAIGKHKNEILIKESADQKKKRIAFDRTARNSLLVMLQNYILKSKYGK
                     EVPVDINNSTILDDDGEITGNIYTIRDITERKKKEEIIRNAAEEWRVTFDSLTDSIFL
                     VNEDGEVIRCNNEATLVLKKEYNDIVGYSVRKLFGQSGDYIYSCFLEIKEELETRIFE
                     QRMGDNWYEGKFDPIIDKKEEFTGAVIILSDISEKKRAQQEIEKYKNHLEELVEIRTE
                     ELKQVNKDLENEISLRWLIAEQMVQMKEYAEAASKSKSEFLANMSHELRTPLNSIIGF
                     AKLLKMGIDPEDSAQYIGNIITSGEHLLSMINDILNLSKMEAGKLKIERKKSDIIQVS
                     AVSMDIVSIQAENKDINLSMSREFEERELFVFADEKRIQQVMLNLLSNAIKFTDAGGK
                     VTVNIRKNSKTCYVDVIDNGCGIDKDMQKTIFDKFNQVDNSLNKRNEGTGLGLAISKS
                     LIESHFGHFYVLSEPGQGSTFTFSLPVYDSEKHEDSLSSLKNNYPQILKEKRLLFILN
                     SQKQITTCVEFMTTNGQEYTILKRSTVSGEALKKDDIGAIVVETAENIGNEQKFVELI
                     KAMNNVPVIAISSTLDMNYKEVFLSMGYDLFYLIPVDYLEIVNDLSKIYGEKR"
     gene            13659..14921
                     /locus_tag="P5123_01180"
     CDS             13659..14921
                     /locus_tag="P5123_01180"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hybrid sensor histidine kinase/response
                     regulator"
                     /protein_id="HRX14906.1"
                     /translation="MNNSKQVLAQAGTFTEPVLIVGDKAENQALLKGICKKINVACDI
                     ASNGKEALELVKDKSYAVYIVDLMMPVMDGRTFVKELKKIYSSAVIIIQTALDTPDTI
                     IELMKKGVYDYIIKPIDPELFQEVLLKSLEYKYLKDLEHQQSLSAGEKIKAQIDWLNY
                     KENRRLSAKDTAESKSIYNVKTSLAQGAGFGTLITLIDIMKASAMETEDGKIAVDKDV
                     VDMMISNNEYCRAQIDGLNFATRIMERDFEFEDYSIGRFISDLPKMTERVIPYLKEKN
                     LKITYPEPTEDAKLRFNKKAISLVVEEMVVNAYKYSRKNSTINLFARFREGYFWFSVK
                     NSIDKEPYGGVPPEYEKLVLEPFFRIHPPDETVSKVERIGFGLGLAVADYVAKKHGGI
                     FIIRDVKDMIKKDPEPCVLAEFLLPVFG"
     gene            complement(14940..17666)
                     /locus_tag="P5123_01185"
     CDS             complement(14940..17666)
                     /locus_tag="P5123_01185"
                     /EC_number="4.6.1.1"
                     /inference="COORDINATES: protein motif:HMM:NF013463.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004016 - adenylate cyclase activity
                     [Evidence IEA];
                     GO_process: GO:0006171 - cAMP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I adenylate cyclase"
                     /protein_id="HRX14907.1"
                     /translation="MDTEILEKNRKAFLLFNDKKVERFKALLPSSNTARILDSMLYLL
                     HTNQNRLPGFVNEFTPHGIHGFSPSESAQKYLKSLFPTISFKSEETKPFIQCIALIGS
                     AGTIAYNKKSDFDFWVCIDKKSVPENRLQHFRKKVQDIQGWAEHHLGLEVHLFINDIN
                     DLQQNIFDEDEEEAFGSTLGALLKDEFYRSSIIIAGKIPFWWAVPLGTNDESYKKIFA
                     ALPPEERNNKYIDIGNLHNITKDDFIGAALFQIVKSLGNPFKSILKLGVLEKYLLESD
                     TVELISHKLKKLVLAGKLSNTVLDSYLLMFYEVFQYYSKTLEDKSSLKILKINLYLKI
                     NPSLSKYTSFKNSSRLPYKALEMYKYTKYWKWNNAVLEDLDSFMNWDFVKVTKFWNQV
                     RRFMLISYQKISRQFVNFDLKSKISESDYKLLSRKIRGYFSFSKDKIERYISFTDTSC
                     EPYLYVDVYKDKDNNIMWRLYKKVGDVSENQSNVVLRIESSLIRLLAWAAINGLYQGG
                     VTRINMINTQNNTNTETIGFTNLMSDLFSKKELKAKNSYYLHESFPLINLIVVNFFIK
                     QKNSILTIDHLYHSSWGESFHKHYTDPDSITDILFDVIKGGVNHKSSFDSYCCFYADD
                     DYKAMVKNIYQLFKKAYMHIVLSKQKNVRFISRVSNNYIIITRKEQRIFTQQCDSITN
                     FYSHLSSSPVKEVATQVYSQNDIYLEKLAYLTENTKSEEITIYYETIGSISFVYIFNE
                     EKNIFASIKPQTELNKFLAHMYHYTHRAIENLKLNNPFAYIDDKSISIKELLTSKGGT
                     FQLKSGHKPLTLQPPSARGYRLICLKNGSYKLGLPDGSNTKPAELKDIVFTCINHSIK
                     ESRVFPQLGLTLIQAVELEGFEHNAAMTSNIFFREKFLIDNLLEQTFKSLKK"
     gene            complement(17882..18889)
                     /gene="holA"
                     /locus_tag="P5123_01190"
     CDS             complement(17882..18889)
                     /gene="holA"
                     /locus_tag="P5123_01190"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: protein motif:HMM:TIGR01128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009360 - DNA polymerase III complex
                     [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit delta"
                     /protein_id="HRX14908.1"
                     /translation="MAQFEKIQGSDKLFSKYEKISPSGAFLFLGEEEAEKEKCVNLFK
                     TNFFDSQPNMQIFYCDTDDFLKGADFVLSEDMFESKKAAVFKKIEAVLKKKQNISLLC
                     DILNRASNSIMIILETNSNRIPSTLLKTGIPFESAIFWKKFENDLLKYIVSELKKRGK
                     TIDSHGASVILSLCGRNIAIIDDALKNIIYGTSNNKIEIEDIKHLIQNNRSLSVFECI
                     DSIFQKDKNSISVIQKTLDGGTHELVIISMIYNRLLQMETYYELKDKGHSTNEALSTL
                     SIQPRQQAFFEQSLRKFSKDSVKNNLIQLAITEKKLKSSPSSKNILANHLMDLLYSLI
                     K"
     gene            complement(19025..19981)
                     /gene="sppA"
                     /locus_tag="P5123_01195"
     CDS             complement(19025..19981)
                     /gene="sppA"
                     /locus_tag="P5123_01195"
                     /inference="COORDINATES: protein motif:HMM:TIGR00706.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006465 - signal peptide processing
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptide peptidase SppA"
                     /protein_id="HRX14909.1"
                     /translation="MKKIYILMFLFLFSSCISIPFSRGIEPLTEQTMVKGSKDKILVI
                     NINGTIHDSQSKKTFLGSAETNISSRVAEELDKAAEDSSIKSIIFRIDSPGGSVTTCD
                     IIHNLITEHKKKSNQYIVAHMGSIAASGGYYLSVTADKIIAHPTTVTGSIGVVAFMVN
                     MEGLLQKIGIEDQTIKSGSAKTMGSPFKKMTKQDRALFQKIIDSMYNRFITVILEGRP
                     NLTRKSLLKIADGRVMISSDALEHGLIDDIGYWKEAVKAAEKGAGINNPTIITYSRPG
                     NYHPNAFTIKSGSESLSLNLISIDSSEFLTKYGISFMYLWKM"
     gene            20212..20568
                     /locus_tag="P5123_01200"
     CDS             20212..20568
                     /locus_tag="P5123_01200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004786672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polymer-forming cytoskeletal protein"
                     /protein_id="HRX14910.1"
                     /translation="MVRTSIQEDTIVNSLIGEGAEFKGEFKINGLLRIDGKFTGTIET
                     DGKVLVGKGGEAITDIEASIVVVGGSVKGNIYATEKVVLLSSGNVSGSIITPSLIMED
                     GVKLEGKCTINKSPVK"
     gene            20569..21015
                     /locus_tag="P5123_01205"
     CDS             20569..21015
                     /locus_tag="P5123_01205"
                     /inference="COORDINATES: protein motif:HMM:NF015822.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF327 family protein"
                     /protein_id="HRX14911.1"
                     /translation="MRIGPAFWPEKNKIKSKKKEASSSSVSGSSFASILEETVETEYS
                     SSIEELMNDLGGQEKNLLDNQTLADLQKYKKTIQKILELITAEGFETKTYRRTRSTNR
                     TDFFIVKKINEKLYRLAAALTSSDNKAFCLVKECEDIRGLIFDLLH"
     gene            21050..22012
                     /locus_tag="P5123_01210"
     CDS             21050..22012
                     /locus_tag="P5123_01210"
                     /inference="COORDINATES: protein motif:HMM:NF024575.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRX14912.1"
                     /translation="MNDIVGHFNQLQHLRSLAFSQRLPQTFLFCGPAGIGKRKAADFF
                     AALLVCQRKDEPPCGICSSCKSLQNGSSQDYIVLEPDEKGRIAVGSDNENGTVRWLTH
                     RLTEKSSSGRYIVIIDGLDRISEAGQNILLKTVEEPGKGVVIIQISSSKSRVLPTILS
                     RSMVINFHSLTAEQISKILKTDPEPHSYEDLALIVSGGSVSVTKDLIEKQILPPILDI
                     ASAIRETVINKAVFNENLSEFDAYNGDFGLYDILINMFHYNIKLVIEKKLQYNRYVEK
                     FYIDDYVLLQRLIKRLLQLKNDLKFNVNSKYALKAALYEMSENI"
     gene            22117..22986
                     /gene="ricT"
                     /locus_tag="P5123_01215"
     CDS             22117..22986
                     /gene="ricT"
                     /locus_tag="P5123_01215"
                     /inference="COORDINATES: protein motif:HMM:NF041131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="regulatory iron-sulfur-containing complex
                     subunit RicT"
                     /protein_id="HRX14913.1"
                     /translation="MEYTCLKMRKNDVIEFVESNNIFLKAGSVCIAETEHGTDMGTVL
                     EIRKRIDIDPAHVKGKVLRAAEKEDYLELPKIEEMENKAFESCRQKAKEKKLEMKLVG
                     VKAVFDKTKIIFYFVAENRIDFRELVRDLAAVYRTRIEMRQIGVRDESRMIGGYGPCG
                     RSLCCTKFQGNFAPVSIKMAKEQNLNLNSLKISGTCGRLLCCLDYEYEVYNMLNSELP
                     QQGTKIKIRNQEYTVESVDTLKESVRIYFEGAFLSIKKRELSYNGKSYSVTNEVIERI
                     THHDEETEDSFGL"
     gene            23063..23362
                     /locus_tag="P5123_01220"
     CDS             23063..23362
                     /locus_tag="P5123_01220"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRX14914.1"
                     /translation="MRAGLIAMAIFIFTLYESCTTISILNLEFIETEEILQLKNSEDL
                     IGVEEVVDRDAVKNVQPRIVERNNKYSKQQYEKSIEKSKRKIMINNCPAQFAEPD"
     gene            23430..24311
                     /locus_tag="P5123_01225"
     CDS             23430..24311
                     /locus_tag="P5123_01225"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: protein motif:HMM:NF013827.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rhomboid family intramembrane serine protease"
                     /protein_id="HRX14915.1"
                     /translation="MTRNPLMSYVVIKIAILNIILSLILGFNTPFVTDYLALNPVSVF
                     YKGYVWQVITYMFGHYNFMHLFLNMFVLFMFGMQVEEAMGKKKFLFFYLFCGIGAGLI
                     IFVTSLIALYQTNVLEYTLGASAAVYAVFVAFAFYYPDAQILLFFVIPIRAKYMVLLY
                     IGFDLYSYMYQTGNISHTGHIGGVIFALIYFLIWGRSSRRGIVSKLVNEVTKKNNEIK
                     EKTALENETQMKKEIISKLRKNSNVERLTEDEYQFVKYCHIMNDEDINYEGDILTFKE
                     ISNKQFITEVRKLIKLD"
     gene            complement(24308..25291)
                     /locus_tag="P5123_01230"
     CDS             complement(24308..25291)
                     /locus_tag="P5123_01230"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF020459.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GSCFA domain-containing protein"
                     /protein_id="HRX14916.1"
                     /translation="MKLFRSLVQLPDFNFTICHKDSIFSSGSCFSEEIANRLHVNGFQ
                     VLKNPSGILFNPVSIETSIERIIHNVQPDSLSEFHENNGIFYSLLNHSSVNAENINSL
                     KNAIITNQKNSAEFIKNCSVLIITYGSSLCYKHIKSGITVGNCHKIPNTEFSKYFLSH
                     NQIINSIINTISLIRETNSKVKIIFTVSPVIHNANGLVDNMRSKASLISAIYEAISEI
                     DSVWYFPSFEIITQELRDYRFYKDDMSHPSDTAVDYVWDLFKNSLFSDESLFIAKECE
                     KLYLNFLHKPFDSKSSEYKLFVEKTIQKQMSISQKLKRNIFEDEMKNFITV"
     gene            complement(25288..26274)
                     /locus_tag="P5123_01235"
     CDS             complement(25288..26274)
                     /locus_tag="P5123_01235"
                     /inference="COORDINATES: protein motif:HMM:NF013718.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase PAP2 family protein"
                     /protein_id="HRX14917.1"
                     /translation="MHQIDIYLTQVIQSFPSFLLPVSQFMDFIGSSNGYILLILIVLF
                     YFDYKLAVNITLLISFSFVVNSILKIGIRIPRPFEVEDSVRNYLNVNRFIGYGMPSGH
                     SQSAMVLWLFANKKILNNILKLLILIFLLIMGLSRIYLGVHWTSQVIVGFFIGYIYWL
                     FSIKFQNTQFIKHFQKMNSQFKMVTITIFSLLITLGALSNMMIFNDFVSSNVFLLGKD
                     VFTGSAFFAGSGCGAVKLLDNYSIRYNRYRFAGTIRFITGTSLILILFIPSKIAINHF
                     SKLDSSPFYSVSLLYALYFTIYIFSFFGFYLVQLFFNRLRLVFLEPKGEVLQ"
     gene            26566..27069
                     /locus_tag="P5123_01240"
     CDS             26566..27069
                     /locus_tag="P5123_01240"
                     /EC_number="2.1.1.63"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015396616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003908 -
                     methylated-DNA-[protein]-cysteine S-methyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylated-DNA--[protein]-cysteine
                     S-methyltransferase"
                     /protein_id="HRX14918.1"
                     /translation="MEHYKVCVKTPLGILEIIESNNKINSVRFKNKNEDILNNTPLLT
                     EAKKQIEAYFKGNLFKFDLPLHIEGTVFQKRVYKSLQMIEYGKTKSYGQIIDAEENAD
                     LGEIKNNKSNHYARAVGRAVSKNNILIILPCHRVINTNGTLGGFSSGLWRKVYLLNME
                     KQYFCKI"
     gene            complement(27099..27950)
                     /locus_tag="P5123_01245"
     CDS             complement(27099..27950)
                     /locus_tag="P5123_01245"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRX14919.1"
                     /translation="MNLKEKILIRKHLVDKDYNAVLALTETISKKEKNGYVFYLMAKS
                     QYLLGYYNESLKSIKRALIYKDTDFDIFLLAGRIYFKLNYFKKAEEFYKYVLKIRPDN
                     VEAYASYAFLLYIIGNDNKTEQYINKAFNIDNDNLFVLLTHFKIILYNNGFKKDAYVL
                     EDVLTHSSSDVYYLVHSALKMAYNNKKRASRQLLIKSSIKKHDPMLYKALSKAISYKK
                     NKKKATLNKKAGLLKIMITIMISSVLFALNKDLFLYWIFLCLIMLISKTVSLVSRLFS
                     NEKGLHE"
     gene            complement(27956..29137)
                     /locus_tag="P5123_01250"
     CDS             complement(27956..29137)
                     /locus_tag="P5123_01250"
                     /inference="COORDINATES: protein motif:HMM:NF012894.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015288 - porin activity [Evidence IEA];
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HRX14920.1"
                     /translation="MKKFLFILLLFFNSLKADPVNLKWKLNEGDRLEVLKTAEVESYI
                     NAKTNAVYSERNIIDLTCKKDNGNLFNVDGTFSVFTKMKNEPTFKKTDENSSSFWIKD
                     NGNFKVPKEFLMPNLRHIPGFPDKKVNIGESWTMDGEIIIDTFSVPFSITMPVKYTLK
                     ETVKSGPITIGHIEYSYLINQDIKPGSVPKDFPLRITGTDKGIIKWDITNNRPLDFFN
                     KYHILFIFNQSGITTTYEWKMNILTKHTIYPFITPEEKTIKKNELEEDIKDKNITIEE
                     NEKGLIIRLGEVLFDFDSANIKPETRKILNDVLTVIKAKYPDREIIVEGHTDNTGNPH
                     YNQQLSEKRASNVANEIINQVEHDKVSYIGHGEKFPIEKNNTAEGRKRNRRVDIIIKL
                     N"
     gene            29219..30706
                     /locus_tag="P5123_01255"
     CDS             29219..30706
                     /locus_tag="P5123_01255"
                     /inference="COORDINATES: protein motif:HMM:NF015380.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="clostripain-related cysteine peptidase"
                     /protein_id="HRX14921.1"
                     /translation="MKKIWVFILFISLTGCFSSSDKDIYHYKIDGLVNKGTWTIMIYM
                     CADNNLETYSLLDVQEMVEGFKTDNGYQIIIMLDRADGSVQGSTNEIGPFEENFSDTR
                     LYMLVDNGIKRLSGKDHFPEITTSGAFEADMGDPENLKKFITFCKNEYKADKYALFFW
                     NHGTGPRSFSNSLPEQRAICVDYGANDSSLGTGEISAYLSKNESVELIGFDACYMGSL
                     EVAYQFAPQHEGFSAKIMVASADEEWAPGWNYKYILNRLSKQNGDRYINEKNYVIEET
                     ENKIIYNPLNMNAADFASIIVEEYYAAVTEQDAFDQVFAAYNLYNIYEIKNIFDSYSG
                     KISLNKEEIVNNRNNFVHYFNFDSDFGQLETPFFDYYDIVNHLEKNLIIVKGDLSNAV
                     DEAVLFSFAGSSLRHKGFVNGCSGLSIFFPDGDTLFDNGNEYFSCWDYQWWYSPFVLE
                     NSDGYGNLYWCRDNASEGNGIVENWFEILEYWFGEESLNENNYIY"
     gene            30684..31214
                     /locus_tag="P5123_01260"
     CDS             30684..31214
                     /locus_tag="P5123_01260"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRX14922.1"
                     /translation="MKTTIYIKNAGFMFIIVLSILSCSKGSSVVRGKSDGGKLRKVEH
                     CKTFNYEIVMVNDKKQGVISIDGQQFPQIFIFVQDKDHAYLFAYDLKKSSVLGYNKTD
                     KIKIKNSDTKITDEELSRGWYLGSNFKVGTPLCWIFVQWNGGSAFISSEGINEIISDE
                     PFNSIFPLSIRNKNLY"
     gene            31434..31868
                     /locus_tag="P5123_01265"
     CDS             31434..31868
                     /locus_tag="P5123_01265"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRX14923.1"
                     /translation="MAVAFVSCGASVPTEVDVIEGDSPAAIAATINKTLEPVVIDGFA
                     FNSARVPSLMVVSGISSVVMSAQKSVPAGFVLTVEGHNDKFEGKSAIGHRRAKAVMYE
                     LRALGMDLGNVRAVNSYDKKLVPEKDAGAPLQRRVTFRVVKK"
     gene            32073..32570
                     /locus_tag="P5123_01270"
     CDS             32073..32570
                     /locus_tag="P5123_01270"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRX14924.1"
                     /translation="MKKLFLMASIVLMTVVLASCGATVPSPVEVTAETPDEEIAKALT
                     ATYLSALVIDEFTFGDQIADLTEVEVVSKAVNACLADATFLELNKKGYKLYAVGHACK
                     FGSRGANMQMGLKRARQVVNELRALNVNVSLMGAKSLGDTVMTDSEAAGHPKQRRVTF
                     AVAKR"
BASE COUNT        10803 a         4882 c         6335 g        10683 t
ORIGIN      
        1 atgggcttta aacggcatag ccgttccaga aatggtaatt tttcgaagtt ccctataatt
       61 tgaaattaat gaattgtgtt atcagattaa aagtgaaaat acttcgatat tcattaatca
      121 aaaatgccat gataatccag ccgatatata caaaagatga tttgtgttat cttttttatg
      181 attaaaattt tgtatgtagc tgtacatcgc agtagtttca atggatttag tagaatccag
      241 aaatatagaa gaggtcacgt ctatggaact ccactgctcg actgtaccag agggtcttgc
      301 ctgccattta tcagcgtatt cgtaaggcat tgtaccatct tcaagtggtg aaaaatttat
      361 ttcacctttt gaaagatgtg ataatgttac atctatcatg gtattgttcc cagaaatgta
      421 tgtgtaaaga atggaaaatt gttcacaatc tccaccgtaa ctgtatccta aaggaatatc
      481 ataattaaac tgattgcgat attggcgttg atacattgtt agatatggaa cattgtcgga
      541 ataggtccag gtatcaatat gggttagttc aaaagcaatt ctgtgaaagg aggagctttt
      601 tgttggcaaa atgtaccagt aaccaatttg agcgcctaat gccgttgggt cttcgtttct
      661 ttcacctgta acttctatct cgttaattaa tagctcagaa aacagaaaat gattaggtga
      721 tggaacaaaa ctggcatcaa tactgaaggt atagctgtga aaatcgggat cgtatgtatc
      781 atgataaata ccaagaggat ttatctggtt aagatcagga aacttacctc cgataaggtt
      841 catttctcct attccaaaag aaaacttgtc aaaggggctt acttcaactc tgtgaaaagc
      901 gaacatctta gagttatctg aaatttttga ctgtttatga cgattattgt atgccgtacc
      961 ccaggttttc tcataatctg tgtaatcgga tcttctatct tgaataaaat cttcttcctt
     1021 acttaactga tgatttgaag accctagcat aaaatagaat ttgaatttag tagaatatag
     1081 atttaattgt atctggtcat agtacggagc cttaccattt tgaaaagtat tacctattag
     1141 accatgaccg attcctccac gaaaccttcc aattgttaaa ctaaaatgat tcttggcgta
     1201 tgatatatat ccttcatcag gggagtccgc gggactgtaa aagtctgggt ttaaagggtg
     1261 gtgggcgttc cagccgtcag aaatactttt agctgaagaa cttaagctat aggtgaattt
     1321 aagatataaa gactcttcat attcaatatc cgcttttatt ttaagtgcgt caattgattt
     1381 gtcatgaagg taaaccatct gtaagggata tgtttttccg taagtagcgt aaggactaaa
     1441 aaatgcagca gcatatggag ttagggtgaa atccatacta gtattaaagc cattgttttc
     1501 attaacggta tctcccgata gacgacttga aaattctctt cgagttattg cgttacaact
     1561 acccgaagga gccttccccg aaataatatg tttaaattcg gctttgcgga attcggcaga
     1621 atttttgtaa atgatttctg cgccgtttga gaaagattga aaagaaatga aaaatataat
     1681 aaatacagat agaactattt tcatggggca accttttcag taatgttgaa tctgcaagag
     1741 ccggtatctt cataagtgtt ccatatctct atcagatatt tttcatcggg tgatagtgat
     1801 atttcgattg tttcatcttc aagataaagg tttttgctga cggcgataac aatctgatca
     1861 gaaaacagtt cggtatcgca atatattcca agagtcagat caaaatctgt actcatgttg
     1921 taaacagaaa gcgtatagat agcctctttg gggggtgaaa agataaaaaa tgcggctgta
     1981 tgagcctcta cctcaatcat ctcttctgaa gaaacagaca caggttctgc ttcaaagtca
     2041 ggaggaggag gtattgactc acatgaaaaa aagaataaat gagataaaac tagaaagatt
     2101 gagaatttgt tcataataac ctaattataa tatatggatt tatattactg gaaaagttgt
     2161 tatcagtttt aataacagat aaaaataata ctaatataaa caatttgaaa gttgctactg
     2221 tttatatggg attaggcatt ttttttatac catctttgaa aatactgaat cgcgctctga
     2281 atccgccgtt tacagaccaa cctcttgatg taatgtacca gtggtctgac tcaataaatt
     2341 cagcaacacg ataaactttg cgataaaaaa tttccccacc taaaaaaaca ctggattcat
     2401 tattaatgat aaaatcaagg tctgctccag atcgtccagt tgttgtcgcg ttattaaaaa
     2461 agaaggtaga aagttctcct ccgccataaa tactgagaat tttgttattt aatggataat
     2521 aacgtaaacc tgcacccaaa ccaaaatcaa agaaaaaatc agttaagccc caggtcaagt
     2581 tagaattaga atagaggtac attccattaa aaaaaacatt tgttgcagat tcaaaaaaag
     2641 gaacacagcc aatgtaaaaa tcctcgtttt caaattcggc tttcattaac tttacgcccc
     2701 aggaagcgct ggctttttca gaagaatccg ctggttgctc ggtttgtcca gttagattta
     2761 aagagataaa taaagtaaga aaggaaatta ataggtattt atttataagc atataataac
     2821 tccgttttct tatttttacg atacaaattg acgaccttat ttcccggtta aggtaatgaa
     2881 cacttttcgg cttcgggggc catcaaactc acagaaaaaa ataccttgcc atgtacttaa
     2941 aaccggtgtt ccgttttcaa ctataatttg ctcggaacag cctatcaagg acgatttaag
     3001 atggcttggc gagtttcctt ccatatgtct aaaagggagt ttttcgaaac caagagagtt
     3061 aagaccggtt accatgtcag taataacatc cggatcggcg ttttcgttta tggtgatcgc
     3121 tgcagttgtg tgcggagtgt atacaataca tatgccgttt ttaatatctg aatctttgat
     3181 gactttacgt acatcggcag taatgtcaat taattgatcg tgcgaatttg tttttaactg
     3241 aatagttgtc ataatcggat tatcctttaa caagaaattg aacagtatgg cgcgttctct
     3301 cttctaatag cactgtctta tttttaagca aagtacggat tgtgttgtcg ttatagtgcc
     3361 aaatggttat caattcaatg ttttcgttat acttcactga aaaatcaatc gagagtgtct
     3421 gaataatatt tttgattctg ctatttgtgt caagacaaag tgaaaaagag agggcgctgt
     3481 tttgcatcat gttaattttt aatgaattat cagcaatcag tgcaaaaata aaactaaggg
     3541 atttttcggt aacaaaagaa aaatccttcg gagaaatagt aagaagtatt aattgctttt
     3601 ttataatgaa agatggaacc tttgtgtgag atgaaatttc tgaaattttt gtgccagacc
     3661 cagatggctg aataaaggat ttaacgtaaa ggggaatacc tttgttttga agcggtttga
     3721 cggttttagg gtgaataact tttgctccat aataggctaa ttcgatagcg tcgtgatatg
     3781 acaaaaaatc tatttttaca gcatcaggaa aaaaaagagg atccgcgttt agaattcccg
     3841 gtacgtcttt ccagacagtt acttctttag catttgtaca aaatgcgaaa atagaggcgc
     3901 taaaatcaga accctctcga cctagagtag ttgtagtttt gtcttctgtg cctccaataa
     3961 atccttgtgt tacataaata tgacccggat gagcctttac ttttaaatca atatttgttt
     4021 ttgtaatatc ccattttatg gatgctgatc ggtaagagga gtctgttatt ataatttttc
     4081 tagagtctag tagggtgttg gaaaagccgt ttttttcaag aaaagatgat aaaaatcttg
     4141 atgacaaaag ttcaccatat gagactatct gatcatactc gtaatcataa ttatcggaca
     4201 cattttcttg taaccgctcg ttaagtttat atagtatatc cataatttca atatgtttta
     4261 gcgaagtatc gccgaatagt ttgatacttt gaagataatg aaaatcacga gtttgtttgt
     4321 ataaattttt tacttcttca atattatgtt caaaatatgc atcggttaat ttttccagaa
     4381 ggttggttgt tttacccata gccgaaatta caataacaag ggtgtcatgc tttgtagttt
     4441 ggtttttaag aatatttgta acagaagaaa aagattcagc gttttttacc gaagctccgc
     4501 caaatttgaa tattttcatg ccggttcctt aattgttgtt taagatataa ttaatcctgt
     4561 attctatcag aaagcaaaat atgattctgt aaagtgtata tttgctacat gatcatgata
     4621 agataattct tcatgatagt acaagagcaa attttagaag ttctattttt aactcttttt
     4681 tgtttgactt tgaatatatt ttatcatgat cattcaataa taagcctaac tgggcttaat
     4741 ttatgtttgc taattgtatg caacaagaat gttgatgtct gttatcggaa cagaatagat
     4801 tatatagagg tgtagttatg aatatctccg aggcgcttaa aaaaagtggg ctttctgtta
     4861 ttgaatccct gttcagtgat ttgcatagcg gtaaagatct tgatatggaa tcggcagaaa
     4921 aattaataga ttcccttata caagaaattt cacaccagaa agaggggatg ctctatttgc
     4981 cagatccgga aaaaacctcg gaatatttga tttaccatac gttacacgtt ttgatgttat
     5041 ctcttatcat gggttacaga acaggaatag agggtatgga gcttagagct cttggtcttg
     5101 gagcagtgct tcacgatctg ggaaaaatta atacacctga aaatctggtc tggaaacagg
     5161 acggcgataa tgagtacgaa aagactgttt tatccgaaca tccggaaatg gttattcact
     5221 ggcttacaca gaaaaatata atttcggatg agatactcgt aataataaaa ggccatcatg
     5281 agcgctatga tgggcttggg tatccgtcag gtgtttcaac ccgtgatttt aaccaatctt
     5341 cgaatattgt ttcaatatgc aattattata gttttttaac aataaacata acggaaaaac
     5401 caggagaaga accgagaaat gctttcttta cgatcagtaa agagatggga aagcgcttct
     5461 ctccaaaaat aatcaatcag tttatttcgg taatgggacc tatgcttatg gatggcccta
     5521 tttaccgtaa aacagcttta gttttgcttg acactaaaga ggtcgctgct gttgttaaga
     5581 ctaacggttt tgccgacaca ttgccggaaa taatcgtttt gacaaactgt cagggtaaaa
     5641 agttggagcg tccggtaagt gttgacctga aaaaagacgg gagccggcgc ataataaaat
     5701 tattaaaggc ctgattaatg cggcaaaata ttgactatct gtatgctgtt ggtgatatct
     5761 tcacctcggg taataatctt gatgacctgt taaaagaagc tatcgagctg actttaaaaa
     5821 agctgtcaat tatcagatta atggtgcaca tatatcagga tgatatcgaa gagatagttg
     5881 ttgatatcta ttcaggttat tctgctgacg agataaaaag gggagtttac aaaaaaggtg
     5941 aaggaattgt tggaaaggta atcagtgacg gtaagcctgt aataattcca agaataggca
     6001 gttgtccgga atttcttaac aaaacgggat cgagaagcac tcaggcagat cttgacggtg
     6061 catttgtatg tgttccggtt agactcaaag ataaaactat aggaacaatc tcggcggatt
     6121 tttcggatgt cgaagaagat ttcttaagct atgctgcaga acagcttgcg gtaattgcaa
     6181 ttttaagtgc ccatgagata agtagccgta tagaaaaaca gaagagtgaa aggcagcttg
     6241 ttgaagagaa ccggcgtttg aacgcacgtt taagcgaaaa aattaaaaac ataaaaatta
     6301 ttggcaattc tgttttaatg catgagctgt ttgaaaaaat agcgttggta agtgaaacaa
     6361 acacaactgt tttgattaca ggagagagtg gaactggcaa agaacttgtc gcggatgcta
     6421 tacagaaaaa cagtttgcga agtgataaac catttataaa agtcaatgtt gcggctttac
     6481 cagagaatct cattgaaagt gaactttttg gacacgaaaa aggctctttt acgggcgctg
     6541 tacagacaaa aaaagggcgt tttgaactgg caaacggtgg aacaattttc ctggatgaaa
     6601 ttggagactt ggcacttcct ttacaggtta aactgttacg ggttctgcag gagagatttg
     6661 tcgaaagagt aggaggagtg gaaactattc ctattgatgt cagggtaatc gcggcaactc
     6721 atcagaatct tgaaaaaaag atagagctta atgaatttcg cagcgatctt tattacagat
     6781 taaatgtgtt tccagttaat gttcctgctt taagagagag aaaagctgat atactggcat
     6841 tggttaacca tttttgcgaa aaattttcaa aagagactca aaagagtata aacagggtct
     6901 cttctgaagc aatcgatatg cttgttgctt atcattggcc cggaaatatc agggaattgg
     6961 aaaattgtat aaacagagca gtaattctcg cagaagaggg ggttattcgc agttatcatc
     7021 tgcctcctac tttacagatt gctaaaaatg tttccgggtc gggttctttt gaaaacatgg
     7081 ttaataattt tgaaaaagag ataatcattg attatcttaa aattacagag ggcaatattt
     7141 ccaaagcagc ggaaatgctt ggaacaacaa aaagaatttt aacatataaa gtaaaaaaac
     7201 ttgaaataga ttgtgaccgt tttaaaaaaa taagatcctg aaattttcag ttacaataaa
     7261 tttataaaca gaggttctaa tgagtaagga taacaagaac aatgaagaaa taacaattcc
     7321 ttcagagagt cttgaaaatg atcttgtttc aattgatcat attctaccga aacagattca
     7381 tattattccc atcaggtatc gtccgatttt tccgggaata attacaccct tgattatatc
     7441 aaaagggcgt ttttctgatg ctatcgatca ggtgcttgat aattctagat caataggtct
     7501 tgttttgctg cgagatgatg aagttgatga tataaagccg cagaatatat ttcagattgg
     7561 tacaggcgca aaaattctta aaaagataaa actaccagat ggtggaataa atgtcctaat
     7621 aaactctttg aaaagattcc gtattgtcaa agttatacag gagaaacctt atcttatagc
     7681 tgaagttgaa tatcttgatg ataagcttcc gcctaaatca aggcgtaacg agctaaaggc
     7741 tttcacgcgt tctgttctaa gtcagcttaa gcagttatcc gaaaacaacc ctctttttac
     7801 ggaagagatg aaacttacca tggttaatgt tgatgaaccg ggtaaaattg gtgattttgt
     7861 atcttcgata cttaatctgg aaaaagaaga gtatcaaaca attctggaga cgcttgatgt
     7921 tgtagaaaga ttacgtctgg ttttacatat gcttcaaaaa gagctggaag ttattgagat
     7981 acagaaaaaa atccagaatc agataaatga taaaatagat aagcagcaaa gagaatattt
     8041 tttaagagaa cagcttaagg ttataaagca ggagcttgga cttgatgctg atgaacgcag
     8101 ccgagagatt agtgaaatgg aagcgattat cagtgaactt gacgtcgaag aagaggtatt
     8161 ggagaagctg actgaagaga tagaaaagct taagaatatg gaacccaatt cttctgaata
     8221 tacagttaca agaaattatc tggatattct tttagccctt ccatggaatg aaaaatctga
     8281 agataatctg gatcttgaca gatcagaaaa aattcttaat aaagatcact ttggtcttga
     8341 tgatgtgaag gagcgtattc ttgagttttt agctgtgagg aaactaaaac cggatgcttc
     8401 ggggtcaata gtttgtcttg tggggcctcc gggagttggt aaaacatctc ttggaaaatc
     8461 cattgctaga gctcttaatc gaaaattttt tagaatgtct cttggtggaa tgcgtgatga
     8521 agccgaaata aagggtcacc gtcgaactta tgttggggca atgccgggca aaataatgca
     8581 gggtattaaa atatgtaaga cgaaaaatcc tgtctttatg ctcgatgaga ttgataaact
     8641 ggggcaaagc tatcagggcg atccggcttc ggcacttctg gaagtgcttg atcccgaaca
     8701 gaattcgaca ttcagggatc actatctgga tgttcctttt gatttatcgg atgtgttttt
     8761 tttgacaaca gccaactctc tcgatacaat accgagggtt ctgcttgatc gaatggaagt
     8821 tatcaggtta tctggttata tttcagaaga aaaatacgaa atagcaaaac ggtatctgct
     8881 tccaaaacag cttgaaaaac atggattgct taaaaacgat gtgaagattg ataaaaacgg
     8941 ttttatgttt ttgattcaat cctatgcacg cgaggcgggt gttcgtaatt tggagcgtgt
     9001 cgtagagaaa atatgtcgaa aaaccgcctc gataaaggcg agaaaaaata aattaccggg
     9061 tgataatctg tcttatgagc aaattcgtga atatttaggt tcggagcgtt ttgtagacga
     9121 tgaaaaattt gaaatagacc gaccagggct tatcaatggt cttgcttgga cctccatggg
     9181 aggagcaatt cttgttgtgg aggccatatc cattaaacag acaaaaaacg ccggattaaa
     9241 acttacaggt caactgggtg atgttatgtc agagtcagca aatattgctt acagtttcat
     9301 aaggcagtat ttatctgaag acgaagagat ggcaaaactt ttcgatagca gcctgattca
     9361 cctgcatgtt ccggctggag caacacctaa agatgggcct tccgcgggta taaccatggc
     9421 atcggcgata tattcgcttg taactaaacg gatagccaaa aaatcatttg caatgaccgg
     9481 cgagttgaca ctttcaggga aagtttttcc tataggtggc ctaaaagaga aattaatagc
     9541 agcaaaacgc gcaaaaataa aaaatattat ttacccgtca ttaaatgaga aaaacctgga
     9601 agaggttccg gataacgtaa aaaaaggatt aaagttttat cctgtttcag agatttcaga
     9661 ggttctggag attctttttc ctaaaaaata gcaggaggga gaaatgaaca ttagaaatat
     9721 gatggaaggg attgtatcat cccttgttct tgaagtgctg aaaaaagagg agagactaaa
     9781 cgagtctaaa gaatattttg aagatatttg tgcatatgtt ttaaaccggt tacccgctaa
     9841 atatgtgaca agtgacaggg gaattcttca tggtatacta gagacaaaat tttctgctca
     9901 acaaaaaagt gatataatca tgcttgttta tgaagctatg acaattattc atgaaaggcg
     9961 tgataaaaat cataaacaca ttcatatttc caagggtgct ccaaacttct tctttcctca
    10021 cctgcttggc gaagttttgg aaaaaacaac tttttccaaa atccctgggg taaaggttac
    10081 tcttctaaag gataagtcgc cggtaaaaat ggtttcagat gggtgggata acccttttca
    10141 tacaaacaat gtaacaggct catactatca tttttggcct ttgtttgatg aagagagtga
    10201 tcctctgtta gaacaaaaat tcttggtaac ttttgagcat gaaaaattca agccttttga
    10261 acaagatatt tatgttagac ccgtctcgtt gacaagtgga gcgatagaaa caagaattgt
    10321 cgctgttgta ttactggact tgaaagaggg tgaagatttg tcgtttcttg aataggtgat
    10381 ggatctttaa cggtttttct cttttattgc tctttccatc tccctcttgt cttgcttttg
    10441 ctttattgag tgccgtttat cgtaaagggt tttacctttg gctaaggcga gctctatttt
    10501 agcctttccg tttttgaagt agagtttaag cggaacaaga gttaaaccct tttccatgat
    10561 ttttccgtaa agcttgctta tctctcgttt gtgtagtaga agttttcgcg gttgtgcagg
    10621 atcatgatta aaccgatttc cctgttcata aggagaaata ttcatttgca ttatataaac
    10681 ttcaccatcc ctgattcttc cgtaagactc gcttatattg acctttccgt ttcgaataga
    10741 ttttacttcg gttcctttta aaacaatacc tgcttcaaaa gtttctatga tatcatattg
    10801 aaaacgggct tttttatttt ttgcaatatc gaattctttc tgttggctca tggtttaaaa
    10861 ctaatctctt tataataaat ggtttttaaa aatactgaaa caaggctaaa ttatggagcc
    10921 attacttgtt atatagagtc aattacaatt ttttattcca aattggtaaa ctcgtcatgc
    10981 aatttgataa ttgagtaggc ctaacctgcg caatcggtac cagcgcagcc tgtggaggca
    11041 agttgctgtc ttgtaaaagt aaaatataac taaaataatg ataatttcaa aacaacataa
    11101 ctttagaagt tcattaaaaa gagcataaaa aatgaattgg taacttgcat taatcaaaac
    11161 taatacatat attgtaggta ataatttgcg aaaatacagt attctttgta tttttttaat
    11221 tcataattaa ttttttataa actcctgata caaaaggata ttttccgaaa tgttgccaat
    11281 atgaaaaaca tacttattgt agaagatgaa aaaattatag ctctggatat caaaaatgcc
    11341 gtacaaaagt atggatataa tgttattggt tctgtaactt ctggtgaaga tgcggtcgag
    11401 ttttgtaaac gctcgttgcc cgatataata ttaatggata ttcatcttga tggagagatg
    11461 gatggtatag atgcggcttt ggccataaaa gaaaatctgg agattcctgt tatttttctt
    11521 acagtttctg ttgattcggt gaccttagag cgtgctaaaa aagttgaacc atatgggtat
    11581 attaacaaac ctgttaaaga cgtcgagata ttcaccacct tggagatggc gcttcacaag
    11641 tttagaaccg aactgaagct taggcaaaac gaacaatggc tgtcagcgat gttaaacgga
    11701 ataggtgaag gcattatctc ggttgatatc gacggtgtga ttacatttat gaaccgtatc
    11761 gctgaaaaga tgaccggctg gattgcttct gatgcgattg gtaaacataa aaatgaaatt
    11821 ttgattaaag agagcgcgga tcaaaaaaag aaacggatcg cttttgaccg aactgcccgt
    11881 aattcccttc ttgtaatgtt gcaaaactat attctcaaat cgaaatatgg aaaagaagtt
    11941 cccgtagata taaataattc tacaatactt gatgatgacg gagaaattac aggaaatata
    12001 tatactatca gggacatcac cgaaagaaaa aagaaagagg agatcatccg taatgcagct
    12061 gaagagtggc gtgttacatt tgattcgtta accgacagta tcttccttgt aaatgaggat
    12121 ggagaggtta tacgctgcaa caacgaagct actttggttc ttaaaaaaga atacaatgat
    12181 attgttggat attccgtaag aaaactattt ggccaatcag gtgattatat ttacagctgt
    12241 tttttggaaa taaaggaaga gctagaaact cgtatctttg aacagcgtat gggtgataac
    12301 tggtacgagg ggaagtttga tccaataatc gataaaaaag aagaattcac gggagcagta
    12361 ataatacttt ctgatatttc tgaaaagaaa cgtgctcagc aagaaattga aaaatataaa
    12421 aatcatctgg aagagcttgt agagataaga acagaagagc ttaaacaggt taacaaggat
    12481 ttggaaaacg agatatcctt aagatggtta atagccgagc agatggttca aatgaaagag
    12541 tatgcggaag cagcgagcaa atcaaagagt gaatttttag cgaatatgag ccatgaatta
    12601 cgtacacctc tgaattcgat tattggtttt gcaaaattat tgaagatggg tatagatcct
    12661 gaagattcgg cacaatatat cggtaatata ataacctcgg gagaacacct gttgtcaatg
    12721 ataaacgata tccttaatct ttcaaaaatg gaagccggaa aactgaagat agaaaggaaa
    12781 aaatcggata ttattcaggt cagcgctgta tccatggaca tagtaagtat acaggcagaa
    12841 aataaagata ttaatcttag tatgtcaaga gagtttgaag agagagaatt gttcgttttc
    12901 gcagatgaaa aacggataca acaggttatg cttaatctat tatcgaatgc tattaaattt
    12961 accgatgccg gaggaaaagt tacggttaat attcgcaaaa acagtaaaac ttgttatgtg
    13021 gatgtaattg ataatggatg tggtattgac aaagatatgc aaaaaacaat ttttgacaag
    13081 tttaaccagg tcgataacag cctaaacaaa agaaacgaag gaacaggact tggcttggct
    13141 atttcaaaat cattaatcga atcgcatttt ggccactttt atgtattaag cgagccaggt
    13201 caaggaagca ctttcacatt ttctctaccc gtgtacgatt cagagaaaca tgaagattca
    13261 ctctcatctt tgaaaaataa ttatccgcag atattaaaag aaaaaaggct gctttttata
    13321 ttaaattctc aaaaacagat tacgacttgt gtagaattta tgactacaaa cgggcaggag
    13381 tatactattt taaaaagaag caccgtctct ggcgaagcct tgaaaaaaga tgatatcggt
    13441 gcaatagtag tcgagacggc agagaatatt gggaatgagc agaagtttgt agaactgatc
    13501 aaagcaatga acaatgttcc ggttattgcg atttcaagca ctcttgatat gaactataaa
    13561 gaggtttttt taagtatggg atacgatctt ttttatctta ttccggtaga ttatctggag
    13621 atagttaatg atcttagtaa aatttatgga gaaaaaagat gaacaactca aaacaggttt
    13681 tagctcaagc tggaacattt acggaaccgg ttttaattgt aggggataag gctgaaaacc
    13741 aggcattgct gaagggaata tgtaaaaaaa taaatgtagc ctgtgatata gcctcaaacg
    13801 gcaaagaggc tttagaatta gtaaaagaca agagctatgc agtttatatt gtggatttaa
    13861 tgatgccggt aatggatgga cgtacctttg taaaagaatt aaaaaaaata tattccagtg
    13921 ctgtaataat tatacaaacc gcgctagata caccggatac aataatagaa ttaatgaaaa
    13981 aaggtgtata tgattacata atcaaaccta ttgatccgga actttttcag gaagtattgt
    14041 taaaatcatt ggaatacaaa tacttgaaag atcttgagca tcagcaatcg ttaagtgccg
    14101 gtgaaaaaat taaggctcaa atagattggc taaactataa agaaaacaga agactctctg
    14161 caaaagatac tgcggagtca aagtctatat ataatgtaaa aacttcgctg gctcagggag
    14221 ctggctttgg aacactgatt accttaatag atatcatgaa ggcttcggcg atggagaccg
    14281 aggacggcaa gattgctgtt gataaagatg tagttgatat gatgataagc aataatgaat
    14341 attgtcgtgc gcaaatagat ggtttaaatt ttgcgacaag gatcatggaa agggactttg
    14401 aatttgaaga ttactcaata ggcaggttta tttccgatct tccaaaaatg accgagagag
    14461 taattcctta cttgaaagaa aagaatctga agatcaccta tccggaaccg acagaagacg
    14521 caaaattaag atttaacaaa aaagctattt ctcttgttgt ggaagagatg gttgtaaatg
    14581 catataaata ctccagaaaa aacagcacaa tcaatctttt tgcccgtttc cgtgaggggt
    14641 atttctggtt ttctgtaaaa aacagtattg ataaagaacc ttacggcgga gttcctccag
    14701 aatatgaaaa acttgtactt gaaccatttt ttcgaattca tcctcccgat gagacagtat
    14761 caaaagtaga aagaatcggt tttggattgg ggttagcggt tgctgattac gttgccaaaa
    14821 agcatggggg aatttttatt attcgtgatg tgaaggatat gataaagaaa gatcccgaac
    14881 cttgtgtttt ggcggaattt cttttacccg tatttggctg aaaataattt tttggtgatt
    14941 tactttttta acgatttaaa cgtctgttca agtaaattat caattagaaa tttttctctg
    15001 aaaaatatgt tagaggtcat ggcagcgttg tgttcaaaac cttcgagttc taccgcctga
    15061 ataagggtta aacccaactg ggggaaaacc cgactctctt ttatggaatg atttatgcag
    15121 gtgaacacaa tgtccttcag ttcggcaggc tttgtatttg atccatccgg caaccctagt
    15181 ttgtatgagc cgtttttaag acatatcaag cgatatccac gggcagatgg aggttgcagt
    15241 gttaagggtt tatgacccga tttaagctga aaagtcccac cttttgaagt taacaactcc
    15301 tttattgaaa tagatttatc atctatgtag gcaaatggat tattcagttt aagattttca
    15361 atcgctcggt gtgtataatg atacatatgt gctagaaatt tgttaagttc cgtttgtggt
    15421 tttattgagg caaaaatatt cttctcttca ttgaaaatat acacgaacga tatggatccg
    15481 atagtttcat aataaattgt gatttcttca cttttagtgt tttcggtaag ataagccaat
    15541 ttttccagat agatatcgtt ttggctgtat acttgggttg ctacctcttt gactggtgaa
    15601 gatgagagat gagagtagaa attggtaatt gaatcgcatt gttgagtgaa tatacgttgc
    15661 tcttttctgg ttattataat gtaattgttt gatactcgtg aaataaatcg aacattcttt
    15721 tgttttgaaa gtacaatatg catgtatgct tttttaaaaa gctgatagat attttttacc
    15781 atggctttgt aatcatcgtc agcataaaag caacaataac tatcaaaaga tgatttatga
    15841 tttaccccac cttttataac atcaaaaaga atatcagtta ttgaatcagg atctgtataa
    15901 tgtttgtgaa aagattcgcc ccaagaagag tggtaaaggt gatctattgt taaaattgaa
    15961 tttttttgtt taataaaaaa attgactaca ataagattaa ttaaaggaaa agattcgtgt
    16021 aaataatagc tattttttgc ttttagctct tttttggaaa atagatctga cataaggttc
    16081 gtaaacccaa ttgtttcggt attggtgttg ttttgggtat tgatcatgtt tatgcgggtg
    16141 actccacctt ggtaaagccc gttaatagcg gcccaagcta atagcctgat tagtgaggat
    16201 tcaatgcgca gaacaacatt tgattggttt tcagaaacat caccaacttt tttgtaaagt
    16261 ctccacataa tattgttatc tttatctttg tatacatcta catagaggta tggctcacag
    16321 gaggtatcgg taaaggatat atatctttca attttatctt ttgaaaagga aaagtaaccg
    16381 cgaattttgc gacttaataa tttataatca gattcggaaa tttttgattt aaggtcaaaa
    16441 ttcacaaact ggcgagatat tttttgatag ctgataagca taaatctacg aacctggttc
    16501 cagaattttg tgaccttgac aaaatcccag ttcataaaag agtctaggtc ttcgagaacg
    16561 gcattattcc atttccagta ctttgtgtat ttgtacattt ccaaagcttt atatggaagc
    16621 cgggagctgt ttttaaacga agtatattta gaaagtgaag gatttatttt gagataaaga
    16681 ttgatcttta gaatttttaa cgatgatttg tcttctagag ttttgctgta atactgaaat
    16741 acttcataaa acataagaag atatgagtcc agaactgtat tggataattt tccagccaaa
    16801 acaagttttt ttaacttgtg actaattaac tcaacagtat cgctttcaag aagatatttt
    16861 tccagaacac caagttttaa tatggactta aacggatttc ctagggactt tacaatctga
    16921 aagagagcag caccgataaa gtcatctttg gtaatattat gtagattccc gatgtcgatg
    16981 tatttattgt ttcgttcttc aggcggaagg gctgcgaata tttttttata cgattcatcg
    17041 tttgttccca ggggaacagc ccaccaaaac ggtatttttc cagcaataat tattgagctt
    17101 ctgtaaaatt catcttttag aagagcacct agggtcgagc caaaagcttc ttcttcatcc
    17161 tcatcaaaaa tattctgttg caagtcgttg atatcgttta taaacagatg aacttcaaga
    17221 cccaggtgat gttcagccca accttggatg tcttgaactt ttttcctaaa atgctgtaat
    17281 ctgttttctg gaacagattt tttgtcaata cagacccaaa agtcaaaatc ggatttttta
    17341 ttataggcaa ttgtcccagc acttccaatt agagctatgc attggataaa gggtttggtc
    17401 tcttcggatt tgaatgatat tgttgggaaa aggcttttca gatatttctg cgcagactct
    17461 gaaggcgaga agccgtgaat gccgtgagga gtaaattcgt taacaaagcc gggaagacgg
    17521 ttttgattgg tatgaagcag ataaagcatg ctgtcaagta tgcgtgcagt atttgaagag
    17581 ggcaaaagag ctttaaatct ttctaccttt ttatcgttaa agaggagaaa cgcttttcgg
    17641 tttttttcaa gtatttctgt atccacgtat aaatcctgtg ttaattagcc catgtgggcc
    17701 ataaatatca ttgttaaaaa ataaatagag gttccatctc tattataaaa cctttcaatt
    17761 ggcaaccttt aataaaacta aaacgtactt ttcaaaaaaa ttttttaaac agtttttcaa
    17821 ttgtttgcag gaaaatgact ataaaattgt agtttaaagg aacttcaaaa aaaatatttc
    17881 gttatttaat aagagaatat aagagatcca ttaaatgatt ggcaagtatg ttttttgaag
    17941 atggtgatga ttttagcttt ttttctgtta tagctagttg aatcaggtta tttttaacag
    18001 aatcttttga aaattttcga agactttgtt caaaaaaagc ctgttggcgc ggttgaatcg
    18061 atagcgttga taaggcttcg tttgttgaat gacctttatc ttttaattca taatatgttt
    18121 ccatttgtaa aagacgatta taaatcatgc tgatgattac aagctcgtga gtacctccgt
    18181 ctaaggtttt ctgaataaca gagatagaat ttttatcttt ttgaaaaata gaatctatac
    18241 attcaaaaac cgataaggat cgattgtttt gtatcaggtg tttgatatct tcgatttcaa
    18301 ttttattatt agaggttcca taaataatgt ttttcaatgc gtcgtcaata atggcaatat
    18361 tacggccgca aagtgagagt ataaccgaag ccccgtgaga atcaatggtt ttgcctctct
    18421 tctttagctc gcttactata tattttaaaa ggtcgttttc gaattttttc cagaaaattg
    18481 cactttcgaa tggaattcct gtttttaata gagtagaggg tatacggttg gaatttgttt
    18541 ccaaaataat cattattgaa tttgatgctc ggttcagaat atcgcaaaga agtgatatat
    18601 tctgtttttt tttcaataca gcttcaattt ttttaaaaac agctgctttt ttgctttcaa
    18661 acatgtcttc agaaagtaca aaatccgctc cctttaaaaa atcatctgtg tcacagtaaa
    18721 aaatctgcat atttggttgt gagtcaaaaa agtttgtttt aaaaaggttt acgcacttct
    18781 ctttttcagc ctcttcttct cccaagaaaa ggaaagctcc ggatggagaa attttttcat
    18841 atttactgaa aagtttgtct gaaccctgaa ttttttcaaa ttgtgccata aaattactta
    18901 actatttccc gattgttccg ataaaaatag agtagaaact catactcctt tgtgttccgg
    18961 tttcttgaaa aggaatacat actatttcaa agcaaacggg atgcgtcgtc ccgttttctt
    19021 ttttttacat cttccataaa tacatgaatg agataccata tttggttaaa aactctgagc
    19081 tgtcaattga gataagatta agagacaggc tttcgcttcc gctcttaatt gtaaaagcat
    19141 ttggatgata attaccgggc cgactgtagg taataattgt cggattgttg attccagcac
    19201 ctttttcagc tgcttttacg gcttccttcc agtagccaat atcatcgata agaccatgtt
    19261 ccaaagcatc agatgatatc atgaccctgc catcagcaat tttaagaaga ctttttctag
    19321 tgaggttggg cctaccttcc aaaatgacag ttataaagcg attatacatc gagtcgataa
    19381 ttttctgaaa aagtgctcta tcttgtttgg tcattttttt gaaaggagat cccatggttt
    19441 tagcagaacc ggatttaatc gtctggtctt cgatgcctat tttttgtaat aacccttcca
    19501 tgtttaccat aaaagcaaca actccaatgc ttccggtaac ggttgttgga tgcgcaataa
    19561 ttttatcagc tgttacagac aggtagtaac caccggaagc ggctattgat cccatatgcg
    19621 ctactatgta ttggttactc ttttttttgt gttcagtaat aagattatgt ataatatcgc
    19681 aagttgtgac tgatccacca ggagaatcaa tacgaaaaat tattgattta attgaagaat
    19741 cctctgctgc tttatcaagt tcttcagcta ctctcgagct gatatttgtt tcggccgaac
    19801 ctaaaaaggt tttcttgctt tgagaatcat ggattgtgcc gttgatgttt atcacaagaa
    19861 ttttgtcttt cgaacccttg accatagttt gctctgttaa tggctcaata cctctggaaa
    19921 acggtattga aatacagctg ctaaacagaa atagaaacat taatatgtaa atctttttca
    19981 ttgttattgc cctctttatt cagaaataga ttatcctctg tttttcttac agcaaatcaa
    20041 cctctttttg tacttgaccg ctttttgtta atgcgatttt actgtagagt aatttttatg
    20101 cttatctgga acttttattt tttattaaag agagctatta tctttccagg tttgtacaat
    20161 gtagttagtt tcccattaaa taattatttg attttgacat caagaggtaa tatggttcgg
    20221 acaagtatac aggaagatac aatagttaac tccttaatcg gtgaaggcgc cgaatttaag
    20281 ggagaattta aaataaacgg gcttttgcga atagacggta aatttactgg cactattgaa
    20341 acagacggaa aagttctggt tggtaagggt ggtgaggcca taaccgatat agaagccagt
    20401 attgtcgtgg ttggaggttc tgtgaaaggg aatatctatg ctaccgaaaa ggtcgtttta
    20461 ttatcaagtg gcaatgtaag cggaagcatt attactccca gtttaataat ggaagacggt
    20521 gttaaactgg aaggaaagtg cacgataaac aagagtccgg taaaatagat gagaatcggt
    20581 cccgcttttt ggcctgaaaa aaacaagatc aagtctaaaa aaaaagaggc ttccagcagt
    20641 tctgttagcg gatcatcttt tgcttctatt cttgaagaaa cagtagagac agaatatagt
    20701 tcctctatag aagaattgat gaatgatctt ggaggtcagg aaaaaaatct tctggataat
    20761 cagactctcg cggatttgca aaaatataag aaaaccattc agaagatact ggagttgatt
    20821 acagccgaag ggtttgaaac taaaacgtac cgtcgaacca gaagtacaaa tcgaacagat
    20881 ttctttattg tcaaaaaaat aaacgagaaa ctttatcgtc ttgcagcggc tctcacttct
    20941 tcggataaca aggcgttttg tcttgttaaa gagtgtgaag atattcgagg tctgattttt
    21001 gatcttttac actaatacag aaacttgaaa ttttaaaaga gatatatttt tgaatgatat
    21061 agtcggacat tttaatcagc ttcagcatct tcggtcgttg gctttttcac aaaggcttcc
    21121 gcagaccttt cttttttgcg gacctgcagg cattggaaag cgaaaagccg ctgatttttt
    21181 cgcggcattg cttgtatgtc aaagaaaaga tgagcctcct tgcggaattt gctcctcttg
    21241 taaatctttg caaaacgggt cttctcagga ctacattgtt cttgagcctg atgaaaaagg
    21301 acgaattgct gttggatctg ataatgaaaa cggaacagtt cgttggttga ctcatagatt
    21361 aactgaaaaa tcttcaagtg gtcgatatat cgtaattatt gatggtttag accgaattag
    21421 tgaagctggt cagaatatac tgttgaaaac tgttgaagaa ccaggaaaag gagtagtaat
    21481 aattcagata agctcttcaa aatcaagggt gcttccgaca atattatcca gatccatggt
    21541 aataaacttt cattccttaa cagccgaaca aatatctaaa attttaaaga ctgatccaga
    21601 gcctcattcg tacgaggatt tggccctgat tgtttcggga ggatccgttt ctgtgacaaa
    21661 ggatcttatt gaaaaacaga tactccctcc catacttgat atagctagtg ctattcgaga
    21721 aacagttata aataaagccg tatttaatga gaatttatca gaatttgatg cttataatgg
    21781 tgattttggt ttgtacgaca ttcttattaa tatgttccat tataacatta aacttgttat
    21841 tgaaaaaaaa ttgcagtata acagatatgt ggaaaaattt tatatagatg attatgtact
    21901 tttgcaaaga ttaataaaaa ggctgcttca gttaaaaaat gatttaaagt tcaatgtgaa
    21961 ttcaaaatat gctttaaaag ccgctttata cgaaatgagt gaaaatattt agctggttac
    22021 acaggtgtaa agactcaatt attatcagta attgctaata tggattttcg ctaaaaatct
    22081 aataagttta tatggttgtt gttaacggag ataattatgg aatatacgtg tcttaaaatg
    22141 cgaaaaaacg atgtaattga gtttgttgaa tcgaataata tattcttaaa agcaggaagt
    22201 gtctgcattg ctgaaactga acatggaaca gacatgggta ctgttcttga aattagaaaa
    22261 cgcatagata ttgatccggc acacgtgaag ggaaaggttt taagagcagc tgaaaaggaa
    22321 gattatcttg agttaccaaa aatcgaagag atggaaaaca aggcttttga aagttgccgt
    22381 caaaaggcta aagaaaaaaa actggaaatg aagttagttg gagtcaaggc tgtatttgat
    22441 aaaacaaaaa tcatatttta ttttgttgct gaaaatcgta ttgattttag agagctggtc
    22501 cgagatctgg ctgctgtata tcgaacccgt atcgagatga gacaaattgg agttcgtgat
    22561 gaatcaagga tgatcggtgg atacggcccg tgtggcaggt cgttgtgttg cacaaaattt
    22621 cagggaaatt tcgcccctgt ttccataaaa atggcaaaag aacaaaatct taatctgaat
    22681 tctttaaaga tatcgggaac ttgcgggcgg ctattatgtt gccttgatta tgagtatgaa
    22741 gtatataata tgttaaatag tgagttgcct caacagggca ccaaaataaa aattcgtaat
    22801 caagaatata ctgttgaatc ggttgatact ttaaaagaat ctgtcagaat atatttcgaa
    22861 ggtgcttttt taagcataaa aaaaagggaa ttatcttata acggaaaatc atacagtgta
    22921 acaaatgaag tgatagaaag aataacacat catgatgaag aaactgaaga tagttttggc
    22981 ttataaataa tgtttacaag taatgattaa ttccgggttt atcatattaa ggtatgtttc
    23041 gaaaaagggg tcaattttac ttgtgagagc cggtttgatt gctatggcaa tctttatttt
    23101 tacattatat gaatcatgta ccactatatc gatattaaac ctagagttta ttgaaactga
    23161 ggaaatatta caactgaaaa attctgaaga cctcatcggt gtagaagaag ttgttgatcg
    23221 agatgctgta aaaaatgtac aaccgcgtat tgttgaaaga aacaataaat acagtaaaca
    23281 gcaatatgaa aaatcgatag agaaatcaaa acgaaaaatt atgataaata attgccctgc
    23341 tcaatttgcc gaacctgatt gattttttgt gtagttatta aatgataata tgatcttgaa
    23401 atatcaaaag cataataaaa ggagatttta tgacgcgtaa tccattaatg tcttatgttg
    23461 taataaagat agcaatacta aatataatat tatcattgat tttggggttt aatactcctt
    23521 ttgtaacaga ttatttagcc ttaaatccgg ttagtgtctt ttataaaggt tatgtttggc
    23581 aagtgataac ttacatgttt ggtcattata attttatgca tctttttctt aacatgtttg
    23641 ttttgtttat gtttgggatg caggtagaag aagcaatggg taaaaaaaag tttctgttct
    23701 tttacctttt ttgtggtatt ggcgctggtt taataatttt tgttactagc ttaattgctt
    23761 tatatcagac aaatgtatta gaatatactc ttggcgcttc agcagctgtt tatgctgttt
    23821 tcgttgcgtt tgctttttat taccctgatg cccagatttt gttgtttttt gttataccca
    23881 tccgggcaaa atatatggtt cttttgtata tcggttttga tctatattca tatatgtatc
    23941 aaaccggaaa tatttcacat acaggtcaca tcggtggagt aatttttgca ttgatatatt
    24001 ttctgatttg gggcagatct tcaagaagag gtatcgtttc aaaactggtt aacgaggtca
    24061 ccaaaaagaa taacgaaata aaggaaaaga ctgcactgga aaatgagact cagatgaaaa
    24121 aagagataat ttcaaaattg agaaaaaata gtaatgttga acgactgact gaagatgaat
    24181 accagtttgt aaaatactgc catattatga atgatgaaga tattaattac gaaggtgata
    24241 ttcttacttt taaggagata tcaaataaac aatttattac cgaagttcgc aaacttataa
    24301 aacttgatta aacagtaata aaatttttca tctcgtcttc aaaaatattt cttttcagtt
    24361 tctggctaat gctcatctgt ttttgaatag tcttttcaac aaacagtttg tattccgaag
    24421 attttgaatc gaaaggctta tgtaaaaagt ttaaataaag tttctcgcac tcttttgcta
    24481 taaataaact ttcatcggaa aataaactat ttttgaataa atcccagaca tagtcaacag
    24541 cagtatctga aggatgtgac atatcatctt tataaaaacg gtaatctctt aattcttgcg
    24601 taattatctc aaacgatggg aagtaccaga ctgaatcaat ctcagatatt gcttcgtaaa
    24661 tagcacttat aagggaagct ttacttcgca tgttgtcaac cagaccgttt gcattgtgaa
    24721 ttacgggact gactgtaaat attattttaa ctttgctgtt tgtttcccta ataagactta
    24781 tagtgtttat gattgaattt attatttgat tatgagacaa aaaatatttt gaaaattccg
    24841 tgttaggaat tttatgacaa ttgccaactg taatcccact ctttatatgt ttgtaacata
    24901 atgagcttcc gtaagttata attaaaacac tacaattctt gataaattcg gcactgtttt
    24961 tttggtttgt aataatggcg ttttttaaag aatttatatt ttctgcatta acagaagagt
    25021 ggttaagcag agaataaaaa attccattat tttcatgaaa ttctgataaa ctgtccggtt
    25081 gaacattgtg aataattcgt tctattgatg tttcaatgga aactggatta aacaaaatac
    25141 ccgaaggatt ttttaaaacc tgaaaaccgt ttacatgcaa tctatttgca atttcttcac
    25201 taaaacatga gccagagctg aaaatggagt ctttatgaca aattgtgaaa ttgaaatccg
    25261 gaagttgaac taaagatcta aaaagcttca ttgtaaaact tctcctttag gttccagaaa
    25321 aacaagccgt aaacggttaa aaaaaagttg aaccaaataa aatccgaaaa atgagaaaat
    25381 gtatattgta aaatatagag cgtataaaag cgatacagaa taaaaaggag aggaatcgag
    25441 tttagaaaaa tgatttatag caatttttga aggtataaaa agtataagta ttaaagatgt
    25501 tccggttata aagcgtattg ttcctgcaaa tctgtatctg ttataacgta ttgaatagtt
    25561 gtctaatagc ttaactgctc cacaaccaga gcccgcaaaa aaagcgctac cagtgaaaac
    25621 atcctttcct agtaaaaaaa catttgatga gacgaaatca ttaaaaatca tcatattgct
    25681 tagagcacca agagttatta ataaggaaaa aatggttatt gtaaccattt tgaactgtga
    25741 attcattttt tgaaagtgct taataaattg agtgttttgg aattttatac tgaatagcca
    25801 ataaatgtat cctataaaaa aaccgacaat tacctgactt gtccagtgaa cacctaagta
    25861 tattcttgat aaacccatta tcaataaaaa gataagtata agtaatttca aaatattgtt
    25921 tagaattttt ttatttgcga ataaccaaag aaccattgca gattgtgaat gtcccgaagg
    25981 cattccatat ccaataaagc gattaacatt taaataattt ctaactgaat cttcaacctc
    26041 gaaaggacgt ggtatacgaa ttccgatttt caagatgcta ttaaccacga aagaaaagga
    26101 tataagcagg gtaatattta ctgcgagttt ataatcgaaa tagaatagaa caatgagtat
    26161 taaaagtatg taaccgtttg atgaacctat aaaatccatg aattggctaa caggtaataa
    26221 aaaagatgga aaggattgaa taacctgtgt aagataaatg tcaatttgat gcataagttc
    26281 ctcgtaaaaa tattatggta atctcaattg attaactttt gttgttctta ttctaagcaa
    26341 ttggctttta gcgcaagaga aaaaaccaac gtgtaatacg aaaactttat ataattataa
    26401 ataataaccg ctatcttcta aaaatggcgc tttcaaagtt cttttatgct catcaaagac
    26461 agtaataatt attttttaca attgtataaa aaagttcaag acattctaaa tgataaaagt
    26521 taattatcag agattacact aatatagttg ccttttggaa agattatgga acattataaa
    26581 gtttgtgtta agacaccctt gggaattctt gaaataatcg agagcaataa caaaataaac
    26641 tcggtacgtt tcaagaataa aaatgaagat attttgaaca acacacctct tttgactgag
    26701 gcaaaaaaac aaattgaagc atactttaaa ggaaaccttt ttaaatttga tcttccattg
    26761 catattgaag gaacagtttt tcagaagaga gtatataagt ctttgcaaat gattgaatat
    26821 ggcaaaacca aaagctatgg ccaaattatt gatgctgaag agaatgcaga tttaggagaa
    26881 ataaaaaaca acaagagtaa tcattacgct cgagcggttg gaagagctgt gtcaaaaaat
    26941 aatattttga ttattttacc ttgccataga gttattaata ctaatgggac attaggaggc
    27001 tttagtagcg gattgtggag aaaagtatat ttgttaaata tggaaaaaca atatttctgt
    27061 aaaatataga agttctgtca agtgaagaaa tgaagaactc attcatgaag ccctttttca
    27121 ttggaaaaca agcgtgaaac cagagacact gttttagata taagcataat aagacaaaga
    27181 aaaatccagt acagaaatag atctttattt aacgcaaaaa gcacagaact gatcataatc
    27241 gttatcatga tttttagcaa accggctttt ttgttcaagg tagctttctt cttgtttttt
    27301 ttataagata ttgccttcga tagggcttta taaagcatag gatcatgttt ttttatacta
    27361 cttttaatta gtaattgccg agatgctctt tttttattgt tatacgccat tttaagagct
    27421 gaatgaacta aataataaac atctgaagaa ctgtgagtca gcacatcttc taaaacatag
    27481 gcatcttttt taaaaccatt attatacaag attattttga agtgagttaa caaaacaaaa
    27541 aggttgtcgt tatcaatatt aaaagcttta tttatatact gttcggtttt gttgtcgtta
    27601 ccgattatgt acagtaaaaa ggcatacgat gcataggctt caacgttatc aggtcttatc
    27661 tttaaaacat acttgtaaaa ctcttcggct tttttgaaat agtttaactt aaaatagatt
    27721 cttccggcta gaagaaaaat gtcaaaatca gtatctttat aaatcagtgc tctttttatc
    27781 gattttaatg attcattata atatccaagt aaatattgcg attttgccat cagataaaac
    27841 acgtatccgt ttttttcctt tttggatatt gtctctgtta atgctaaaac agcattataa
    27901 tctttgtcga caagatgttt tctgattaat attttctctt ttaggttcac ggatcctagt
    27961 ttaactttat tattatatcg acacggcggt ttctttttct tccttcagcc gtattgtttt
    28021 tttcaatagg aaatttctct ccatgtccta tataagatac tttgtcatgc tcaacttgat
    28081 taataatttc gttagcgaca tttgatgctc gcttttctga taattgctga ttataatgag
    28141 ggttgccagt attatccgta tggccttcta cgataatctc tctgtcggga tattttgctt
    28201 taatcacagt caatacatca tttagtattt tacgtgtttc tggcttaatg tttgctgagt
    28261 caaaatcgaa aagaacttct cccaagcgaa taataagtcc cttttcgttt tcttcaatag
    28321 taatattctt atctttaata tcttcttcaa gttcattttt tttaatggtt ttttcttctg
    28381 gagtaataaa tggataaatt gtatgttttg tgagaatatt cattttccat tcataagtgg
    28441 ttgttatacc gctttgatta aaaataaaaa gaatatgata tttgttaaaa aaatcgagag
    28501 gccggttatt tgtaatgtcc catttgatta ttcctttgtc ggtacctgtt attcttaacg
    28561 gaaagtcttt tggtacggat ccgggtttaa tatcttgatt tatcagatat gaatattcta
    28621 tatgccctat tgtaatagga cctgatttta cagtctcttt tagtgtatac ttaaccggca
    28681 ttgtaattga aaaaggtacg ctaaaagtat ctataattat ttcgccatcc attgtccagg
    28741 attctccaat atttactttt ttatcgggaa atccaggaat gtggcgtaaa ttaggcatta
    28801 aaaattcttt tggtactttg aaatttccat tatccttgat ccaaaatgat gaactgtttt
    28861 cgtcagtttt cttaaaagtt ggttcgtttt tcatttttgt gaatacagaa aaggtgccgt
    28921 caacattgaa caagtttcca ttatcttttt tacaggtaag gtcaattata tttctttcgg
    28981 aatatacagc attagtttta gcattaatgt aagattcaac ttcggcagtt tttagaactt
    29041 caagacgatc gccttcgtta agtttccatt tcaaattaac cggatcggct ttgagagaat
    29101 taaaaaaaag tagtagaata aacaggaatt ttttcataat atacctcaat agatgttcgg
    29161 attatatatt attggaatta aacagaacag aacaaatttc ttaaaataag gtaaaaatgt
    29221 gaaaaaaatc tgggttttta ttctttttat ctctcttaca ggatgttttt cttcctcgga
    29281 taaggatatt tatcactata aaatcgatgg cttggttaac aagggtacct ggaccattat
    29341 gatatatatg tgtgctgata acaatttaga aacatactca cttttggatg ttcaagagat
    29401 ggtagaaggg tttaaaactg ataatggata tcagattata ataatgttag atagggctga
    29461 tgggtcagta caaggaagta cgaacgaaat tggtcctttt gaagagaatt tttctgatac
    29521 aagactttac atgctggttg ataatggaat aaaaagactt tcaggaaaag atcattttcc
    29581 tgaaatcact acttctgggg cttttgaagc tgacatggga gatcctgaaa atcttaaaaa
    29641 atttatcaca ttttgtaaaa atgaatacaa agctgataaa tatgcccttt ttttctggaa
    29701 tcatggaacg ggtcctcgtt ctttttctaa tagcttacct gaacaaagag caatatgtgt
    29761 tgattatgga gcaaatgatt catctttagg tactggtgaa ataagtgcat atctttcaaa
    29821 aaatgagagc gtagagttaa taggttttga tgcctgttac atgggttctc ttgaggttgc
    29881 ctatcagttt gctccgcagc atgagggatt ttcagcaaaa ataatggtag cttcagcaga
    29941 tgaggaatgg gcgccaggtt ggaactataa atatatatta aacagattgt ccaaacagaa
    30001 tggtgataga tatattaatg aaaaaaatta tgttattgaa gaaaccgaaa ataaaataat
    30061 ttacaatcct ttgaatatga acgctgcaga ttttgcttca ataattgtcg aggaatacta
    30121 tgccgcagta acagagcagg atgcttttga tcaggttttt gcggcttata atctttacaa
    30181 tatttatgaa ataaaaaata tttttgactc atattctgga aaaatatcgt taaataaaga
    30241 agaaatagtt aataaccgaa ataattttgt acactatttc aattttgatt ctgattttgg
    30301 tcaacttgag actccttttt ttgattatta tgatattgtt aaccatcttg aaaagaattt
    30361 gattattgta aaaggggatt tgagcaatgc ggttgatgag gctgttcttt tttcttttgc
    30421 cggatcaagt ttaaggcata agggatttgt taatgggtgt agcggattat ctattttttt
    30481 tcctgatggg gatactcttt ttgacaatgg caatgaatat ttttcgtgtt gggactatca
    30541 gtggtggtac agtccgtttg ttttggaaaa cagtgatggt tatggcaatc tgtattggtg
    30601 tcgtgataat gctagcgaag gtaatggaat tgtagaaaac tggttcgaaa ttttggaata
    30661 ctggtttgga gaggaaagtt taaatgaaaa caactatata tattaagaat gctggtttta
    30721 tgtttataat tgttttgtcg atattaagct gtagcaaagg atcaagtgtt gtgcgaggta
    30781 agtcagatgg cggaaaactt agaaaagttg aacattgcaa aacttttaat tatgaaatag
    30841 tcatggttaa tgataaaaaa cagggtgtta taagtataga tggtcaacaa tttccacaaa
    30901 tatttatttt tgttcaagat aaagatcacg cttacctttt tgcctatgat ttaaaaaaaa
    30961 gttctgtttt gggttacaac aaaactgaca aaatcaaaat taaaaatagt gatacgaaga
    31021 ttacggatga agagttgtcg aggggttggt accttggatc caattttaaa gtaggtactc
    31081 ctctttgttg gatttttgtg cagtggaatg gaggtagcgc ttttattagt tcagaaggta
    31141 taaatgaaat tatctctgat gagccattta attcaatttt tccactaagt ataagaaaca
    31201 agaatttata ctaaatatat gctttgattt tatatcaaaa atgtagatat tcttgttatc
    31261 aacaagaatt aaacatacat aaagaacgta ttgataggat ataatattca attaattgta
    31321 aattaatgaa aatttacttg attttcttcg cgttctttat tatttgtaat gcaactatct
    31381 aaattaagga gtaaaaaatg aaaaaagttc ttttatctct tgctgcatta tttttggcag
    31441 ttgctttcgt ttcttgtggt gcttcagttc caacagaagt agatgttatt gaaggggaca
    31501 gccctgctgc aatcgcagca actattaaca agactttaga gcctgtagtt attgatggtt
    31561 ttgctttcaa tagcgctcgt gttcctagtc ttatggtagt ttctggcatc tctagtgttg
    31621 ttatgtctgc acagaaaagc gttccagctg gtttcgttct tactgttgaa ggtcacaatg
    31681 acaagttcga aggcaaaagc gccattggac atcgtcgtgc gaaagctgta atgtatgagc
    31741 ttcgcgctct tggcatggat cttggtaatg ttagagctgt taatagctat gacaaaaaac
    31801 tagttcctga aaaagatgca ggtgcgcctc tacagcgacg tgtaactttt agagtagtta
    31861 aaaagtaact gttcttaagt agatagttat ttgttatttc tattggagag ggcagtgccc
    31921 tctctgatat tttcttaaaa attcgcgttg ttttgatgtt ctgatttttt aagagtgctg
    31981 tgttgttata tttataaata ggcttaagta tttttataga tgcaaatata aaaatagaaa
    32041 ataaatttta aaactgtttt aaggagaagt ttatgaaaaa gttattttta atggcttcaa
    32101 ttgttttgat gactgttgtg cttgcttctt gtggagccac tgttccgtct ccagttgaag
    32161 taactgcgga aactccggac gaagaaattg caaaagcttt aacagctact tatctgtctg
    32221 ctcttgtaat tgatgagttt acttttggtg atcaaattgc tgatcttaca gaagtggaag
    32281 ttgtgtctaa agctgtaaat gcttgtcttg cagatgctac ttttcttgag ctgaacaaga
    32341 aaggctacaa gctttatgct gttggtcatg cttgtaagtt tggttcaaga ggcgcaaata
    32401 tgcaaatggg tcttaaacgt gcccgtcagg ttgtgaacga gcttagggct cttaacgtga
    32461 atgtttcgct tatgggtgca aaatctttag gtgatacagt aatgactgat tctgaagctg
    32521 ctggacatcc taagcagaga agagttacat ttgctgtagc taaaagataa ttattttatt
    32581 tagatagttt tgatataaag ggccttcatt ttgaaggtcc tttttgtgtc aaaaaaacct
    32641 gaaatttgaa ttaattgtct tgacctgcga aagggtgcat aatatttttg ctctgaaatt
    32701 gac
//
LOCUS       DAOTGZ010000008        30867 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_3064
            NODE_1654_length_30867_cov_7.197423, whole genome shotgun sequence.
ACCESSION   DAOTGZ010000008 DAOTGZ010000000
VERSION     DAOTGZ010000008.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868088
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 30867)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 30867)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:22:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,785
            CDSs (total)                      :: 2,751
            Genes (coding)                    :: 2,732
            CDSs (with protein)               :: 2,732
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 12 of 19
            Pseudo Genes (internal stop)      :: 5 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..30867
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1654_length_30867_cov_7.197423"
                     /isolate="AMR_MDS_3064"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..764)
                     /locus_tag="P5123_01275"
     CDS             complement(<1..764)
                     /locus_tag="P5123_01275"
                     /inference="COORDINATES: protein motif:HMM:NF013109.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015116 - sulfate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0008272 - sulfate transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SulP family inorganic anion transporter"
                     /protein_id="HRX14925.1"
                     /translation="MNQQFVPKLFSLVKSGIPAKQIITDIQSGVLVGIIALPLALAFA
                     IASGVSPERGIITAIVAGFIISFLGGSRVQIGGPTGAFIVLVFSINAQYGITGLLTAT
                     AMAGIMLIIMGALRMGDLLKYIPYNLIAGFTGAIALIIFSTQMKNFFGMQIDDLPAEF
                     AGQWISYLKHLSTINIYSASIGVMTICVILLVRKKIPRVPGALVCVIIFTSIVYLLKI
                     PVETIGSRYGEISGSIGFEGLTVPSVEMIKALLLPS"
     gene            complement(1105..2121)
                     /locus_tag="P5123_01280"
     CDS             complement(1105..2121)
                     /locus_tag="P5123_01280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012501567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SPFH domain-containing protein"
                     /protein_id="HRX14926.1"
                     /translation="MGLWDKAKGQLRSVIEWNNPSEDIIFYRWSSDGDEIKNASKLIV
                     NPGQGCIFVYEGKVEAFFEEEGIVELKTANIPFWTTISKFMQKFESEHKVGIYFYKKT
                     QILNQKWGTPSVIKYMDPKYNFEVGLRAYGNYAFQINDPGHFFCNIMGVGTEYTTAQF
                     RDVMGSKIIEPLTDYFAEAGFGYSDIDKERQEISAGIKERLLKEFSDLGFEIIDFKIE
                     GTSFDDNTMKRINRIADMTAEAEALKRVGMNYADQQKLEAMKLAAQNEGGGAGLGMGM
                     GAGMGMGQMMAGMMGSQQSSPQVSNDSGDPTAKLKKLKEMMDMELISKEEFEQKKKEI
                     LGSL"
     gene            complement(2482..2622)
                     /locus_tag="P5123_01285"
     CDS             complement(2482..2622)
                     /locus_tag="P5123_01285"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRX14927.1"
                     /translation="MTILLIAGGSLLVGFGIICYFLSDRRSLERKDSETWENLWMSRL
                     DR"
     gene            complement(2789..4273)
                     /gene="lysS"
                     /locus_tag="P5123_01290"
     CDS             complement(2789..4273)
                     /gene="lysS"
                     /locus_tag="P5123_01290"
                     /EC_number="6.1.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002188823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004824 - lysine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006430 - lysyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lysine--tRNA ligase"
                     /protein_id="HRX14928.1"
                     /translation="MSEKPADIREQRIEKIKKMRESGVNPYPNRFERKDKIADVRAAF
                     DAEKPVNVVVAGRIKSKRVMGKASFGNVEDFSGSIQFYAARDLMGEEGYNLYKTHDLG
                     DHVGIEGETFLTQKGEMSIKVKRLVLLSKCIQPLPVVKEKDGQVFDEFSDTELKYRRR
                     YVDLIVNQKTRRDFLLRSRIVSSIRRFLETDGFLEVETPMMHSVVSGAAAKPFITHHN
                     TLDLELYLRIAPELYLKRLIVGGFERVFEMNRNFRNEGIDTRHNPEFTMVEIYQAYAD
                     YKDMMDLTERLFTGLAEEYFESTEIPYQGQTINLKGPWRRVRFVDAIKEVTGLDFSLI
                     KTASEAVAAAKSVGIETDESMGIWKIADEVLGERVEKTFQNPTFLIDYPKELSPLSKS
                     FEDNPDYVERFELFIAGREIANAFTELNDPFDQKERFEGQSQMRDKGDDEAMMVDDDY
                     ITALEYGLPPTGGLGIGIDRLVMLFVDTASIKDTILFPLLRPEV"
     gene            4552..4863
                     /locus_tag="P5123_01295"
     CDS             4552..4863
                     /locus_tag="P5123_01295"
                     /inference="COORDINATES: protein motif:HMM:NF017415.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phasin family protein"
                     /protein_id="HRX14929.1"
                     /translation="MSNILKDIGELFISAGDEIEKKTHSFKEKHQEDFDKFKTNVKQG
                     KEDFQKKFKDEVSSAKERLSDALSNFGSSSKKEIDELKAKLDELSAKLDELSKSDGNN
                     S"
     gene            5311..5778
                     /locus_tag="P5123_01300"
     CDS             5311..5778
                     /locus_tag="P5123_01300"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRX14930.1"
                     /translation="MRRFKLIIFIMLSTYCTTLLSEETDLAAGIRLEAGKDGNYGATA
                     RYKLGEPSYIEGLFLFNEEGFEISALYEFQIRPEFFPESLFLFFGGGAHIGFWGEDDP
                     TVFGPDGIVGVLYSAKDYPINFSLDWHPLYNIVSEKKKRMWGNKLGISARYCF"
     gene            5929..7812
                     /locus_tag="P5123_01305"
     CDS             5929..7812
                     /locus_tag="P5123_01305"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRX14931.1"
                     /translation="MSNLDKTRDNIKTSQLSESERKAIFQKFVEHGGKVEDTKPRRHK
                     TFDRNKQKEFLQKSEERKNKLAQQKPRIKTKKIISENISNKQSVSSQGPNFSQNPVIK
                     FLTLLRIRFNLLIHGITDFSGLEIKSSFIKKFNQIYKPSIIDLQLVYLDIFKKTGHSI
                     SEQIVEKLDRERPLLFELIEFAANIYDREITSDIIDEYIAFGEKDYKTFEMAHALTEY
                     FKRIYLIINFQDTLINAYEKAISHHSSLVKKTDSLNQHKRKIKQSINIICNKLFPQLY
                     WLFCFFNSGIVSLYDISIIDHMLGLTADMKPGSRQPHSPSRLSAELAVKTKEQKEREI
                     SSEMEQAREEIKKKSAPVVPDEVKRGLKLMTQIKRNELAKKFIKSNYIISEYKSDPIV
                     KSFLLFREFDEEYSILFTTNKIKISTPSDRRNSSDYRLKLSELYNKIRHIDNQYREYF
                     STSELYHETEEDKPISQNQYYHYSKRLTELENDLKKRSRDIRNDLKNYFEAIIDLFEE
                     LHKDMEGQQKIVINPQQEIELDYELEGNKKLKGKKIYQALLEVIDFSYAFVYRLSSGN
                     DLSESRITKEAVPIQESSTTGETQPPAEATEPVIPTITANPNDTEISDQSDSIISELE
                     NFL"
     gene            complement(7859..8686)
                     /locus_tag="P5123_01310"
     CDS             complement(7859..8686)
                     /locus_tag="P5123_01310"
                     /inference="COORDINATES: protein motif:HMM:NF017266.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016645 - oxidoreductase activity, acting
                     on the CH-NH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MnmC family methyltransferase"
                     /protein_id="HRX14932.1"
                     /translation="MKEKIILNKTEDNSLTLYLPEYGENTHTIHGAYTEALNKHIIPS
                     GICNKSGDLCILDIGFGLGYNILALLDSFYNSPFSYLRIISFEKEKGIADYLDMVKFN
                     DKRDDLYAMVKDAFNKGSFSNDKIKIEICFGDARQNIKKISLKNSVDAVFHDPHSPGK
                     NSELWTINFFKHLHACMKDDAYLTTYSFAPQIRYGLIKAGFFVGSWTGNCFEKEGTIA
                     SKSIFKDCFSDDYIRLLEADKRSTPYLDNETMDLERETIRINRIEAAKKRKELIQQI"
     gene            complement(8661..9074)
                     /locus_tag="P5123_01315"
     CDS             complement(8661..9074)
                     /locus_tag="P5123_01315"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRX14933.1"
                     /translation="MKKRTKNRVIIVVFISILVLSCSGLSTKEESYHDNSLNVYVRIE
                     GRRVDEMSDNRLIQELEKKARNRALGLMIIVIEDRIADHIYKERIISNLRTFEMKSEL
                     LSFTINDYYCDGYFKFKTKGFEESLIDYERKNNIK"
     gene            complement(9098..9391)
                     /locus_tag="P5123_01320"
     CDS             complement(9098..9391)
                     /locus_tag="P5123_01320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013504793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF167 domain-containing protein"
                     /protein_id="HRX14934.1"
                     /translation="MNLKKKNNCYQLTFKVTPNSSKNALEKDADGNLRLRLVAPPVDG
                     KANKACIAFFSKTFKIPKSSIKIVKGETSRYKTILFDGCYEKDIAIIIDRIIA"
     gene            complement(9369..10241)
                     /locus_tag="P5123_01325"
     CDS             complement(9369..10241)
                     /locus_tag="P5123_01325"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRX14935.1"
                     /translation="MKKILLLSVIFLTTNLFPYNWESKDYSVRRSASRDIARVTDPAG
                     RVFFVKGDSRVNKARAEDIEKLVMLFYKMKYVSVDRIIFNVKGSSLRILVVPRAYKYN
                     ESDLLPYMTAGLVFDYSADVLNYNFRLKSGEIFMKLTGVFIDEEMLSKKIEEALKDPG
                     AYVARRDSEYLLNKLDELEVKVMQLEEQNKRIIKNFSDYQKATNQYIRNLQTEDERIR
                     FAIIAFQNDRFFAGEKPVKPEIVEAVIKLRLRNPSLDIEDFMDKVEEAGIEVSKKEVN
                     IILIAYRNEFKKEK"
     gene            complement(10241..11707)
                     /locus_tag="P5123_01330"
     CDS             complement(10241..11707)
                     /locus_tag="P5123_01330"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRX14936.1"
                     /translation="MGNEKQVKRDLIEKSKSEFLPAEFHDPDSGVVTYSIEEEYAKTQ
                     KNRSLRLYGLVLLFFAAIAVASLVWIVVLQRQGDNLVVDISDFKDIKALDVVNRIKEL
                     ETRINELKYELKAIYEKRDDEIARLNREFRSQREALLAVDDEVLHDEPTGSEKESSIF
                     DDEPSSSEEASDTGVGVAEESSIVTTSSSKDEVVAVDSRFRSKSGTIRDKYDLLAKNK
                     EEEIRKAEEELENLRKKAGADVESAGAILGEKDELYNLQMNKMRKEYEDQKSETTRKH
                     QDELDQIVNRYNPHFNESRLQAIIGRRVINVRIPSEVLSGIEKDLQRERAVTKDDISQ
                     LRRSVSDEMALLKKLKTVPYTNSVPRSLLHMESFTKITVAIYDRIVTKLIERVRRKNS
                     MINAYNYAFSSKVKRDGENGFVLDARNKNRIIMYIDDTYEIRDGDTAYIFRSDESEIA
                     TVTIEKGPDLYYGKVVTMSADARINPFDKIMLKVVRER"
     gene            complement(11686..13392)
                     /locus_tag="P5123_01335"
     CDS             complement(11686..13392)
                     /locus_tag="P5123_01335"
                     /EC_number="4.6.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012437.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009975 - cyclase activity [Evidence IEA];
                     GO_function: GO:0016849 - phosphorus-oxygen lyase activity
                     [Evidence IEA];
                     GO_function: GO:0032555 - purine ribonucleotide binding
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate/guanylate cyclase domain-containing
                     protein"
                     /protein_id="HRX14937.1"
                     /translation="MKRTVRGLFFRSVNRTRVVPVRLKIIVIFVMFIILSNLSTNFIN
                     LMYNRNHQLMLMKKILAKDLKDLHVYSNTRYEISTFNGNIEASLNNIKSKALYEMKNR
                     KAVFLAVKPNGQILLDASHEGPVWNEFDPVALEKMKESMEKEGQGFLTVNNGDFEYFG
                     IYKYNSKWDAFLFRAEEYGEFNKESVETIKMISLVILVGTIVLTIVGILIIGHLLRFI
                     GILTNSIMKMVKNQQLELIHLENAPNDDITYLGMAFNSLSSTIETLLQIFKRFTNKDI
                     AYKAYKERIIRLEGTQKELTILFSDIKGFTTITETLGTDIIKLINMHYDKAIREIIRY
                     DGAIGSIIGDAILAMFGTMDEVVTQNKSLSAVAAAYELQSIAESLRLEMTKKKESIIR
                     VKGGLTREEEQVYNALMIEIGVGLDGGEVFYGNIGSYVRMTNTVIGDNVNSASRLEGL
                     TRIYSVPVICSEYVKNDILQNTSERGITFVEIDIVQVKGKTIGKKIYWPIPEQYMSGS
                     LKKDIARFSKALKLYYAGKWVSANKEFKQVNLPLAREFINRTESKAAPKNWTGIWAMK
                     SK"
     gene            complement(13666..14391)
                     /locus_tag="P5123_01340"
     CDS             complement(13666..14391)
                     /locus_tag="P5123_01340"
                     /inference="COORDINATES: protein motif:HMM:NF024824.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HRX14938.1"
                     /translation="MQKMIFSVIMVFSLTVSLSAGYDEALELYNRGEYMKSIYAVADV
                     LLIEKDMDPSSENYKLRFLAAHCHWRLGNYDKAMTHFIKTREIRPDSLDPLIDMALMN
                     LEANRNSHAEAYAKAVLDKDPKNSLALYVFGGVMASRGNYAAAQDYYQRIIAAETEHY
                     AAYNALGKVLMSQKKYGNAETAFSTAVALNSNSAEVYNNLAICYYRLEKVDLAKETIE
                     KALTLDPDNDSIRRNKQLIDKGI"
     gene            complement(14572..16536)
                     /locus_tag="P5123_01345"
     CDS             complement(14572..16536)
                     /locus_tag="P5123_01345"
                     /inference="COORDINATES: protein motif:HMM:NF024753.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PQQ-binding-like beta-propeller repeat protein"
                     /protein_id="HRX14939.1"
                     /translation="MRKKTVILIAILCFAVGFSVLNASDWPVYKGNIFFTGNNDEIVV
                     PNGNLKWLYQAEDRVYNPIVSDGFVYFIDQKANLYCLNENSGKLIWKINVRSISDQFK
                     KLSRSAGKIKYPLIKGDYIYLTDPVAVYCINKKNGRVLWARTAYNMSPSASMDVKKGD
                     LSGTTTNAFVDGIYSDPVLTGNEIYYGTRKVFVSRDVRDGKEKWKNSSVKSYSGFPTF
                     YDDYLIVQSMDYQTMEYKVLLLEGATGKTIWEKRLPQPFKIFPPVVYKRRIYIPVSQS
                     LYCLDIDNGKELWKKDYGGIISSLPGFTDRAVIFTIDNKRLVITDPENGSIENSIDIG
                     DKSSPGYVMVRDQFYIAYNDKGGVNRAYGSVRAINYSDKSELWKFKTPFPGAVSQPVA
                     SNGILFLPAGNYLYAIGAEAYPRVVTGGSSVYDEPESGSDSTATEKDPTGYADKEISP
                     PDDKKIKMRDLDLDVTDEGGNKVPAYADIEQRDSEGRPLYKERQPVRDGKIKVPDGDN
                     VHVVVGSDGYIPEGFDLDRDEKKRDVKLEKIESGKTYVVKDVTFEYNKAYLRKSSLPI
                     LNRLLDIMVKNPGLKLEIRGHTDSKGNDKYNQKLSERRADAVSEFMIKNGISPERLRS
                     IGFGETKPVASNDTEEGRQKNRRTEFIFKE"
     gene            complement(16757..16951)
                     /locus_tag="P5123_01350"
     CDS             complement(16757..16951)
                     /locus_tag="P5123_01350"
                     /inference="COORDINATES: protein motif:HMM:NF016028.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SlyX family protein"
                     /protein_id="HRX14940.1"
                     /translation="MISDERIEELEIKFSYLDESVRILNETVIEKDREIDSLKKRVKI
                     LENSLEVLGEEISNEKPPHY"
     gene            complement(16944..17693)
                     /locus_tag="P5123_01355"
     CDS             complement(16944..17693)
                     /locus_tag="P5123_01355"
                     /inference="COORDINATES: protein motif:HMM:TIGR00530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipid acyltransferase family protein"
                     /protein_id="HRX14941.1"
                     /translation="MKFLYSLYRIIIYPFLLLMTALISICVIIVSVFSEKAAYVLPIV
                     HSRLLIFFIPIRLKIFGKKNLEKGQSYVFVINHNSALDIGAVYAGLKRDIRWVMKKEL
                     TDVPVFGTACRKLGNIPIDRKNPNKAVESINIAKQKIRDGVCLAFFPEGTRRDDVLLG
                     SFKKGAFHFAIDIGIPVVPITLVGMEKIMPKTSRLFYSGKVKMFIHSPIASTSYTKQS
                     VDEFSDKARLVIESTLKREYPDNYFKKVIND"
     gene            17986..20406
                     /gene="leuS"
                     /locus_tag="P5123_01360"
     CDS             17986..20406
                     /gene="leuS"
                     /locus_tag="P5123_01360"
                     /EC_number="6.1.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014624035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004823 - leucine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006429 - leucyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="leucine--tRNA ligase"
                     /protein_id="HRX14942.1"
                     /translation="MNENYDFKSIETKWIKKWDESKEFRVKEDPSFPADKRLYVLDMF
                     PYPSGSGLHVGHPEGYTATDIFTRFKKMNGYNVLHPMGYDSFGLPAENYAIKTGTHPA
                     VTTRTNIDRFREQIKTFGFAYDWDREISTCEPDYYKWTQWIFLQLYKKGLAYESEMPI
                     NWCPDCLTGLANEEVKEGCCDRCGSQIERRRIRQWVLKITEYAERLLADLDGLDWSES
                     IKAMQQNWIGRSEGAEVDFVIEKTGEKLRVYTTRPDTLFGATYMVLAPEHSAVQQITS
                     AEQKEQVDNYIIEAAKKSDLQRTELNKDKTGVFTGAYAINPVNGSKIPIWISDYVLIS
                     YGTGAIMAVPAHDTRDFEFAKKYDIPIIQVVSLDGSLNDLDEAMTQEGIAVNSGQFDG
                     LKTLEFKSKIIEWLEKECKGQKAINYKLRDWIFSRQRYWGEPIPLVHCDKCGIVPVKE
                     EDLPLTLPEVDSYTPTGTGESPLAAIKEWVNTKCPVCDGDAKRETNTMPQWAGSCWYY
                     LRYMDPANSKALVDPDILDYWGSVDLYVGGAEHAVLHLLYSRFWHKVLLDCGVVSFKE
                     PFQKLRNQGMILGENNEKMSKSRGNVINPDDVIAEFGADSLRMYEMFMGPLAADKPWN
                     TSSIFGVKRFLDKVWRLQDKISSDSQLSPEALKMVHKTIKSVTYKIDNLDFNTAISQL
                     MILLNTLQKEPAVTRETYQTLLTLLHPFAPFITEELWEQYGNGKSMLKTNWPSFDPDL
                     AVDDVITVVFQVNGKVRSREELSADSSKEEMLEIAKNNDKIKQYLDGKTIIKEIVVLG
                     KLVNLVVK"
     gene            20648..20821
                     /gene="nrdD"
                     /locus_tag="P5123_01365"
     CDS             20648..20821
                     /gene="nrdD"
                     /locus_tag="P5123_01365"
                     /EC_number="1.1.98.6"
                     /inference="COORDINATES: protein motif:HMM:NF024984.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008998 - ribonucleoside-triphosphate
                     reductase activity [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic ribonucleoside-triphosphate reductase"
                     /protein_id="HRX14943.1"
                     /translation="MKTKMVPTEIYSRVVGYYRPVNQWNKGKQSEFADRKEYDVNTLT
                     LASEQTEKEAIAV"
     gene            complement(20859..21713)
                     /locus_tag="P5123_01370"
     CDS             complement(20859..21713)
                     /locus_tag="P5123_01370"
                     /inference="COORDINATES: protein motif:HMM:NF020132.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxymethylpyrimidine/phosphomethylpyrimidine
                     kinase"
                     /protein_id="HRX14944.1"
                     /translation="MPARLILNFLENVEKSQSPAKKIIVQCAVLSVRLNSKFLHIISA
                     TDSSCGAGTQQDVRVAMDHNVWPVTTVSGVTAQTFDGVIGVEPVSNKIFEKQLECGLS
                     FKCGGLKIGALLSIDQMKMVWHYIKKNPELPVVIDPVFSPTEGRPFYSAEMQLFLANE
                     LLPLSCLVTPNLEELKILTQMEVLTVIETVKIIGDKYKGKPLFYIKGGHEETASKYIK
                     EYLVGEEIYTLKKARWNLQYTHGTGCAMATATACNIIKGKSIKKSAFRASKYITNLYK
                     RINRELCE"
     gene            complement(21604..22863)
                     /gene="thiC"
                     /locus_tag="P5123_01375"
     CDS             complement(21604..22863)
                     /gene="thiC"
                     /locus_tag="P5123_01375"
                     /EC_number="4.1.99.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013450881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphomethylpyrimidine synthase ThiC"
                     /protein_id="HRX14945.1"
                     /translation="MTQISRIKKGDISPDIEEVARKEQRSIEFISEGIIAGKIVIPKN
                     CNRTFSAEGVGKGLKTKVNANIGTSPDCNNVANEMKKLETAIKYGAHSVMDLSIGGDL
                     NGMRRKVLENSPIMVGTVPLYAVFAETVRDGGSIKDIDGDRLFGAIEQQAKEGVDFMT
                     VHCGVNCETFSSHDPDKRAAGIVSRGGALTRRWMRETGKDNPLYTQFDRLLDIAYKYD
                     VTISLGDGLRPGGMPDATDPLQLTELAALGRLVDRCRERDVQVMVEGPGHVPIQDIPF
                     NMEIQKKVCKEAPFYVLGPLVIDCAPGYDHITAAIGGAVAAWHGADFLCYVTPAEHLT
                     LPDEDDVREGVVASVIAAQAGDVAKGVPGAYDRNRLMTEYRKKLDWEKMYACSIDPEL
                     PRKRREESESSEKDYCTMCGSLCALKQ"
     gene            complement(22867..23481)
                     /gene="thiE"
                     /locus_tag="P5123_01380"
     CDS             complement(22867..23481)
                     /gene="thiE"
                     /locus_tag="P5123_01380"
                     /EC_number="2.5.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010662570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004789 - thiamine-phosphate
                     diphosphorylase activity [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine phosphate synthase"
                     /protein_id="HRX14946.1"
                     /translation="MEDFGLYVIITDPDYPVSKITEICVKYNVGMLQLREKELCDKEL
                     LKTLELMLSITKGSSTKVVINDRVDLAIASGADGYHLGQDDIPFVNAREVAGNRNLVC
                     GLSTHSLEQAKEAVKLKPDYIGFGPVYPTFAKKNPDAPVGCDQLKQVLDLSDCPVVAI
                     GGINRDNISEVLETGAKNIAMIKGLVKSANIEEEISFYSSLLAR"
     gene            complement(23483..24253)
                     /locus_tag="P5123_01385"
     CDS             complement(23483..24253)
                     /locus_tag="P5123_01385"
                     /EC_number="2.4.2.59"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005857953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfide-dependent adenosine diphosphate thiazole
                     synthase"
                     /protein_id="HRX14947.1"
                     /translation="MEVTVSRGIIRSFFAKLEDNLDIDVAIVGGGPSGLTAAYYLAKA
                     GKKVALYESKLAPGGGMWGGAMLFNQIMVQKEAIDIIEEFDIRYTEYDDNNVMLDSVE
                     ATSALINRAVKNGVVIFNGVKVEDVVVHSETRKVNGVVINWTPVVNIGMHVDPLVVRA
                     KAVLDGTGHPCEIVSTLARKNDIKLNTPTGGVMGECSLSVVEGERTTIENTKEVYPGM
                     YVSGMSANGVSGSFRMGPIFGGMLMSGRKVANLIMEKL"
     regulatory      complement(24307..24400)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00059"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="TPP riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="thiamine pyrophosphate"
                     /db_xref="RFAM:RF00059"
     gene            24835..25086
                     /locus_tag="P5123_01390"
     CDS             24835..25086
                     /locus_tag="P5123_01390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007293028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008219 - cell death [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system
                     prevent-host-death family antitoxin"
                     /protein_id="HRX14948.1"
                     /translation="MTQITANKLKTRGVSILNEETSDGNEVIITVRGENKFVVIPIEK
                     YNYLRECELEAALLETKKNIKDGKYFIYQSANVTPRAAI"
     gene            25498..25944
                     /locus_tag="P5123_01395"
     CDS             25498..25944
                     /locus_tag="P5123_01395"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRX14949.1"
                     /translation="MKLENDFTDMISYLIEFDCRFIVVGGFALAFNGFIRNTGDIDFF
                     VEPTIKNSEKVFNALKSFGAPIQDINPHDFTQKGTIYQIGLPPIRIDIINSIDGVSFE
                     EAYATKKIKKIDGLKVPYLSIELLKKNKTASGRSKDLFDIEELNKL"
     gene            25941..26174
                     /locus_tag="P5123_01400"
     CDS             25941..26174
                     /locus_tag="P5123_01400"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRX14950.1"
                     /translation="MNSANAKEKRKQERLKKWSSCLIRNNEVIAGKEITNNNEPDIDR
                     CLSQLHELRRMAWPELDEDYRMEKSIAKIIRPS"
     gene            complement(26355..26798)
                     /locus_tag="P5123_01405"
     CDS             complement(26355..26798)
                     /locus_tag="P5123_01405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018205510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Rrf2 family transcriptional regulator"
                     /protein_id="HRX14951.1"
                     /translation="MKLSTRSRYGVRLLIELGLNQDKGSVQLNSIAQSQDISEKYLGQ
                     LVIQLKGAGIISSERGKTGGYRLVKEARLLTLREVVEKLEGGLSLVDCVDDTVDCNRQ
                     NICVTRDLWKELSECMIDFLESVTIQDLVDRTVKARLKNNISYDI"
     gene            complement(26795..27724)
                     /gene="cysK"
                     /locus_tag="P5123_01410"
     CDS             complement(26795..27724)
                     /gene="cysK"
                     /locus_tag="P5123_01410"
                     /EC_number="2.5.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010262619.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004124 - cysteine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006535 - cysteine biosynthetic process
                     from serine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine synthase A"
                     /protein_id="HRX14952.1"
                     /translation="MKIYNDITETIGKTPLVRINKMNKKGYAEVVVKLESFNPVSCVK
                     ERIAKSMIESAEQEGKINSDTVIIEPTSGNTGIGLAFVCAVKGYRLILTMPDTMSAER
                     RHILKIFGAELVLTEGAKGMKGAIEKAEELARENKNSYIPQQFDNPANPQVHRETTAE
                     EIWSDTDGKVDIFVAGVGTGGTITGVGEFLKQKNSAVKIIAVEPIESPVISGGKPGPH
                     KLQGIGAGFIPKNLNVDVIDETIQVKQTDAGDIAREAAKREGILVGISSGAALWVALE
                     VSKRPENKDKRVVVLLPDTGERYLSTWLFEDSK"
     gene            complement(27957..28094)
                     /locus_tag="P5123_01415"
     CDS             complement(27957..28094)
                     /locus_tag="P5123_01415"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRX14953.1"
                     /translation="MMLFGKAHLIKIQSGLFGEILDYSMSRATAARLLIYGKNGMTEI
                     M"
     gene            complement(28225..29064)
                     /locus_tag="P5123_01420"
     CDS             complement(28225..29064)
                     /locus_tag="P5123_01420"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRX14954.1"
                     /translation="MKNSYRILALLFFFVACTNDVDDNNNKRSFLMGFTPWPYDSTVE
                     AVEWIYDAINKNGDIISHHMEEGVPWPESYAGESFADEFQTEINSRVERYQADKKILL
                     SVSPLNMGRDGMALYRGISEGMVLPSPWSDYALNSNEVKTAYLKYTERMIELFKPDYL
                     IIGIEVNLLIRNSPVLWEPYIELHNYVYTQIKKKYPDLPTGVSVFCVPYFPDWSPEDD
                     LEEQLIGLKQLEASSDFIAFSVHPFMSALTAEKFPDDYLKKLFEITTKPVAVSESSYP
                     AQY"
     gene            complement(29061..30017)
                     /locus_tag="P5123_01425"
     CDS             complement(29061..30017)
                     /locus_tag="P5123_01425"
                     /inference="COORDINATES: protein motif:HMM:NF013775.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphotransferase"
                     /protein_id="HRX14955.1"
                     /translation="MNIQIAKEICKKYLNTDPLECYRFTTGLRNYSFYLQTENDKYVV
                     KICKQDNLQSLKNSVYWIGRLKTLNIQIPDIVTMDLNCQTPFVIYTYIEGDDLGNVYQ
                     RLNESEKKEIAQNLVNIQREVKKLGQADGYGYLSNMTDPNKKDCWLDVIKEHINRSRS
                     RIKENGDFSESYADKVEKLLPDFFSYFETIPPLAFFDDATTKNVLINDGKFSGIIDFD
                     YICFGDELYTIALTKMSLLNSNYSLTYIDYWLEYRGLNQSEQAALDLYTLIFCIDFMS
                     EKGMCFNKAQPLKVSEHEVEQLKNIYDEYMTKIMLNAQRTGL"
     gene            complement(30047..30538)
                     /locus_tag="P5123_01430"
     CDS             complement(30047..30538)
                     /locus_tag="P5123_01430"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRX14956.1"
                     /translation="MLGNKVTVKKPFVDIKEYLLSELTGWFVYEAESSSALKLYFYNN
                     RKIEIPLIGKKTRFLAKTIIENNFEIIKQKGIDAIENGPVEHKTCFTVYLIDKEYIEV
                     RKRYRSQKYIYSMCFEKKLGSISSRYFFEFKYNGKKITINSYAFRKNQGLFWGFVNYI
                     HNS"
BASE COUNT         9811 a         6373 c         4707 g         9976 t
ORIGIN      
        1 aatgacggta acagtaatgc tttaatcatc tctactgacg gtacagtaag cccttcaaac
       61 cctatgctgc cgcttatctc accatatctt gaaccaattg tctctaccgg aattttcaat
      121 aaatatacaa tgctggtaaa aattataaca caaaccagag ctcccggaac acgaggaatt
      181 ttttttctaa caagcaatat cacacaaatt gtcatgacac caatagaagc agaatagata
      241 ttaattgtag aaagatgttt taaataggat atccactgcc cagcaaattc tgctggtaaa
      301 tcgtcaattt gcataccaaa gaaatttttc atctgagtcg agaagattat aagcgcaata
      361 gcacctgtaa atccagcaat cagattgtaa ggtatatact taagaagatc acccatccgc
      421 aatgcgccca ttattataag cataattccg gccatggctg tagcagttaa taaaccagtt
      481 attccgtatt gagcattaat tgaaaaaaca agaacaataa atgctcctgt tggtccccct
      541 atttgtacgc gacttccacc aagaaacgaa ataataaaac ccgctacaat tgccgtaata
      601 atgccacgct ccggagatac tccgctggca attgcaaaag ctaaagccag tggtaacgca
      661 attattccga caagaactcc cgactgaatg tctgttatga tttgttttgc gggaatacct
      721 gacttaacaa gtgagaataa ttttgggaca aactgttggt tcatattgca cctcatacaa
      781 ccatatataa tcacaatggt ttgttaaaaa tcttgttaaa acctcaaata attttctttt
      841 caacaaagtg catatgtaaa aagtctagct acttttcatt cataaaaagt gatacaggac
      901 aaatgaacaa tgtaacttgt gaaaaattta tctatgttat tggcaacaag attattagtt
      961 ataaaaaaag atcagcctaa gctgatcttt acatacataa acgcggttca tatttgtaca
     1021 atgttttttg agtaaatatt tcaattgcaa aaaaaatgtt ttgcaaacga ttgcttgttt
     1081 tactatttcc tggaaacaat cattttataa tgaaccaaga atctcttttt tcttttgttc
     1141 aaattcttct ttcgaaataa gctccatatc catcatctct tttaatttct tcaacttagc
     1201 agtcgggtca cctgaatcat ttgaaacctg aggcgaagac tgctgacttc ccatcatacc
     1261 ggccatcatc tgtcccatgc ccattcctgc acccataccc ataccaagtc cggcaccacc
     1321 gccctcattt tgagcagcaa gtttcatagc ttcgagcttt tgctgatccg catagttcat
     1381 accaacacgc tttaaagctt ctgcttctgc agtcatatca gctatgcgat taatacgttt
     1441 cattgtatta tcatcaaaac ttgttccttc gattttaaaa tcgataattt caaaaccaag
     1501 atcagaaaac tctttaagca gtcgctcttt aattcctgca gatatctcct gtcgctcttt
     1561 atctatatca gaataaccga atccagcctc ggcaaaatag tctgtcaacg gctctataat
     1621 cttagagccc ataacatcac gaaattgagc tgttgtgtac tcggtgccta cacccataat
     1681 attgcaaaag aaatgcccag ggtcattgat ttgaaaggcg taatttccat atgcacgcaa
     1741 tccaacctca aaattatatt ttggatccat atattttatt acactgggag ttccccattt
     1801 ctggttaaga atctgtgttt tcttataaaa atagatacct accttatgct cactttcaaa
     1861 cttttgcata aacttgctga ttgttgtcca gaaaggtata tttgctgttt taagctcaac
     1921 aataccctct tcttcaaaaa atgcctctac tttaccctca tatacaaaaa tacacccttg
     1981 tccggggtta actatcagct ttgatgcatt tttaatctcg tctccatcag aactccatcg
     2041 ataaaaaata atgtcttcag acggattatt ccattcaatt accgaacgta attgcccttt
     2101 tgccttatcc catagtccca ttttttttct cctgatttat tcagataacg gatcgaatag
     2161 ataagtagtg atcctttttt tagtgaataa ctatcagata acatataatc atggagtcag
     2221 tcaaatatta attaacaata cttgccctaa aaattattaa ctgctatatc tgttaagaag
     2281 gaaggggcca ttgatatgga tactttaaaa cattgataaa aaccaaaagg gaacttcgaa
     2341 aaattagctg aaaattttaa actaatagtg tatctagcta atcgtaaatt ttaaagttcc
     2401 cttagtgaat atctggtttt ttatcaagat taaatattga aaagtggtta aacagcagtg
     2461 tacagaaaaa tcgggataat atcaacggtc caaacggctc atccaaagat tttcccatgt
     2521 ttcagaatct ttacgctcaa gagaacgtct atcggaaaga aaataacaaa taatcccaaa
     2581 accaaccaga aggcttcctc cggcaataag cagtatagtc atccaaaacc tctcataaca
     2641 agacaaactg ataacattac agatgttcaa ataaatccgc acaggtttgc tgtcaagcat
     2701 tatgaaaaaa aataaccctt tcgggctatt ttgttttatc aagacaggaa atctctaaaa
     2761 tacaaatcat aaggcattat tggccaaatt atacttccgg tcttaacagc ggaaaaagaa
     2821 ttgtatcctt aatagaagca gtatcgacaa aaagcataac aagcctgtca ataccgatac
     2881 ctagcccccc tgttggagga agtccatact caagagcagt tatataatca tcatcaacca
     2941 tcattgcttc atcatcaccc ttgtctcgca tttgagattg gccttcaaat ctctcttttt
     3001 gatcaaatgg gtcattcaac tcggtaaacg cgttggcgat ctcgcgaccc gctataaaaa
     3061 gctcaaatct ttcaacataa tcaggattat cctcaaaaga ttttgataac ggagataatt
     3121 ctttggggta atcgattaaa aaagtcgggt tttgaaacgt tttctctaca cgttcgccca
     3181 aaacttcatc agcgattttc catattccca tggactcatc agtttctatt ccaacagatt
     3241 ttgcagcggc tacagcttcc gaagcagttt ttataagcga aaaatctaat cctgttacct
     3301 cttttatggc atctacaaac cttacccgtc tccaaggtcc ctttaggttt atggtctgtc
     3361 cctgataagg tatttcggta ctttcaaaat actcttctgc caatcctgta aaaagacgct
     3421 ctgttaaatc catcatatcc ttataatcag cataagcctg gtaaatctcg accatagtaa
     3481 actcaggatt gtgtctggta tctattcctt cattacgaaa atttctattc atctcaaaaa
     3541 cacgttcaaa accacccaca attaaacgct ttaaataaag ctcgggagct attcttaaat
     3601 atagttcaag atccagtgta ttgtgatgtg ttataaaagg tttagcggcg gcaccactaa
     3661 caaccgaatg catcattgga gtctctactt ctaaaaaccc atctgtttca agaaaacgcc
     3721 taatagatga aacaatacgg ctacgtaaaa gaaaatctct tcgtgttttt tgattaacta
     3781 tcaagtcgac atatcttctt ctatatttta actctgtatc tgaaaactcg tcaaagacct
     3841 gaccatcctt ttcttttaca acgggcaaag gttgtataca cttggaaaga agaaccagtc
     3901 tttttacttt aatagacatt tcaccttttt gagttaaaaa ggtctcacct tctatcccga
     3961 catgatcacc caaatcatga gttttatata ggttataccc ctcctcaccc attaaatctc
     4021 ttgccgcata aaactgaatt gaacccgaaa agtcctcaac atttccaaac gatgctttac
     4081 ccatgaccct tttggattta attcttcctg ctacaactac attaacaggt ttttcggcat
     4141 caaaagccgc tcttacatcg gcaattttat ctttacgctc aaaacggtta gggtaaggat
     4201 ttacaccgct ttcacgcatt ttctttattt tttctatcct ttgttctctt atatcagccg
     4261 gtttctcact cattacaaag taccccttat cataaataca aaattaatct taaatttctc
     4321 agatttaatg tataacttat ccatctgcaa acacaatttt atttcataaa acccgttttg
     4381 acttaaaagc tattatttaa acaaatagat ctcataaaat agcgacattg tctccattgt
     4441 tgaataatta atccatattt tattccgtat ttttaattgc aagttaataa atatcttgaa
     4501 ttatttctaa atataaatac ccttaagtta gttacaataa aggagatcat tatgagtaat
     4561 attttaaaag acattggcga gttatttatt tcggctggag acgaaatcga gaaaaaaacc
     4621 cattctttta aagagaagca tcaggaagat tttgacaagt tcaaaacaaa tgttaagcag
     4681 ggtaaggaag attttcagaa aaaattcaaa gatgaagtat catctgccaa agaacgtctg
     4741 tcggatgcgt taagcaattt tggttcttct tcaaaaaaag agatcgacga gctaaaagct
     4801 aaactggatg aattgagcgc aaaacttgac gaactttcaa aatccgatgg taataacagt
     4861 taacaaccag aagattctaa aacggccgat caaaatgcaa tatcttgttt ataaattgat
     4921 atctttgcaa tctaagatat tcaagtgatt tgtcagttta tactgatatt attttttagt
     4981 taaaaatttc tgaatactcc tgtttacttc aaaagatagc agttcaaact ctggaataat
     5041 taatattttt accagataaa tttctacaag cagttcatat atttacaaat ctaattacgc
     5101 aacaatgaat catacagttc tgaaaaaaac ttgtcaaatt cgacactttc caattaaata
     5161 tcaactggtt ttattattaa attctctatt gttacctagt taacattttt gatcaactga
     5221 taataatgga atttcagcaa atgcaattag caatttcttt agatttttct atcataagtt
     5281 gtccgacaac aattcaattg gagagttatt atgcgaagat ttaaacttat catatttatt
     5341 atgctttcaa cttactgcac gacattgctt tcagaagaaa cagacttggc tgccggtatc
     5401 agacttgaag ctggaaaaga cggcaattat ggtgcaacag cccgttataa attaggtgag
     5461 ccttcttaca ttgaaggact tttccttttc aatgaagaag gtttcgaaat ttctgccctc
     5521 tatgaatttc aaataaggcc tgaatttttc cctgaaagtt tattcctctt ctttggagga
     5581 ggtgcccata taggtttctg gggggaggat gacccaactg ttttcggccc agacgggatt
     5641 gttggagttt tatactctgc aaaagactac cccattaatt tttctcttga ttggcatcct
     5701 ctatataata tagtttcaga gaaaaaaaag cgcatgtggg gcaataaact tggaatttca
     5761 gcccgctact gcttttaata aattgatttt tttattagcc tgttcttgtt aggcatgtgt
     5821 attaagttta taaccttatt acgcaaaatg gattgacttt ttgtcctttt gtttactatt
     5881 aatgtattat actccttttt ttatgatttc atattttcag gtatgctaat gtctaacctt
     5941 gacaaaacac gagataatat aaaaaccagt caattgtctg aatcagagcg taaggctatt
     6001 tttcagaaat ttgttgaaca tggcggcaaa gtcgaggaca caaaaccaag acgtcataaa
     6061 acttttgaca gaaacaagca aaaagaattc ttacaaaaaa gtgaggagcg caaaaataaa
     6121 cttgcgcaac aaaaaccaag aataaaaacc aaaaaaatta tttccgaaaa catctcaaac
     6181 aaacagtctg taagctcaca agggcctaat ttttcacaaa atcctgtaat aaaattttta
     6241 actctcctta gaatcagatt caatttatta attcatggca ttaccgattt tagtggcctt
     6301 gaaataaaat catcgtttat taaaaaattt aatcaaatat ataaaccatc aataattgat
     6361 ttgcaactgg tatatcttga tatttttaaa aaaaccggtc attccatctc tgaacagata
     6421 gttgaaaaac ttgatcgtga acgccctcta ctttttgagt taatcgagtt tgctgcaaac
     6481 atttacgaca gggaaataac atctgacatt attgatgaat atatcgcttt tggtgaaaaa
     6541 gattacaaga ctttcgaaat ggctcatgct ttaactgaat atttcaaacg aatatatctt
     6601 ataattaact ttcaggatac attaattaat gcatacgaga aagctatttc tcaccatagt
     6661 tctttagtaa aaaaaaccga ctctctgaat caacacaaac gcaaaataaa acagagcatt
     6721 aatatcatct gtaacaaatt atttccgcaa ctatattggc tattctgttt ttttaattcc
     6781 ggaattgttt cactttacga catatccata attgaccata tgctgggact tactgctgac
     6841 atgaaacctg ggtcaagaca accgcacagt ccaagtagat tgtctgccga gcttgccgta
     6901 aaaaccaaag agcaaaaaga acgcgaaata agttcagaga tggagcaggc tcgcgaagaa
     6961 attaaaaaga agagtgctcc tgttgttcct gacgaagtca aacgcggact aaaattgatg
     7021 acccagatta aaaggaatga acttgcaaaa aaatttatca aaagtaatta cataatttct
     7081 gaatacaagt cagatccaat cgtcaaatct ttcttattat tccgcgaatt cgatgaggag
     7141 tattcaatct tatttactac aaacaagatt aaaatttcca ctccatctga ccgacgtaac
     7201 agctcagact acagattaaa actgtcagaa ctgtacaaca aaattcgcca tattgataat
     7261 caatacaggg aatatttttc aacttcagag ctttaccatg aaacagagga agataaacct
     7321 atttcacaaa accagtatta tcattacagc aaaagattaa ccgaactaga aaacgatctt
     7381 aaaaaaagat caagagacat acgtaatgat cttaagaact acttcgaggc aattattgat
     7441 ctttttgaag aactgcataa agatatggag ggccaacaaa aaattgtaat taatccgcaa
     7501 caggaaattg aactagacta tgaacttgag ggaaataaaa aacttaaagg taagaaaatc
     7561 tatcaggcac ttcttgaagt catagacttc tcttacgctt ttgtttacag actctcttct
     7621 ggaaacgatc tttcagagtc aagaattaca aaagaggctg ttccgataca ggaatcttcg
     7681 accacgggtg aaacgcaacc tccggcagaa gcaacagaac cagttattcc caccattaca
     7741 gctaatccaa acgatacaga aatttccgac caatcggatt caataatcag cgaattagaa
     7801 aattttcttt aaaaacaact aagtcatcta attcgtttta tcatcaaaac agcttctatt
     7861 aaatttgttg tattagttct ttcctttttt ttgccgcttc tatacgattg attcttattg
     7921 tttctcgttc aagatccatt gtttcgttat caagatatgg tgttgatctt ttatccgcct
     7981 ccaaaagtct tatataatca tcagaaaaac agtctttaaa tatacttttc gaagcgatag
     8041 ttccctcttt ttcaaaacag ttccctgtcc atgatccaac aaaaaaaccg gctttaatca
     8101 aaccataacg aatttgtggt gcaaatgaat aggttgttaa ataagcatca tctttcatgc
     8161 atgcatgcaa atgtttaaaa aaattaattg tccaaagttc gctgttttta cccggagaat
     8221 gaggatcatg aaaaaccgcg tctacactgt tttttagact aatttttttt atattttgtc
     8281 gtgcgtcacc aaaacaaatt tcaattttta ttttatcatt actaaatgaa cctttattaa
     8341 aagcatcttt aaccattgca taaagatcat cccgcttatc gttaaactta accatgtcaa
     8401 gataatctgc tatacctttc tccttttcaa aacttattat tcgcaaataa ctaaaggggc
     8461 tattataaaa actatccaat agagccaaaa tattatatcc aagaccaaat cctatatcta
     8521 gtatgcataa gtctccactc ttattacaaa taccagaggg aataatatgt ttgtttaatg
     8581 cttcggtgta ggctccatgt atggtatgtg tattttcacc atattcagga agataaagtg
     8641 tcaggctatt gtcttctgtt ttatttaata ttattttttc tttcataatc gatcagcgat
     8701 tcttcaaaac cctttgtttt aaatttaaaa tatccatcac aataatagtc atttatagta
     8761 aatgacaata gctcagactt catctcaaat gttctcaaat ttgaaatgat tctttcttta
     8821 taaatatgat cagctattct atcttctata acgataatca ttaaacctaa agctcgattt
     8881 cttgcttttt tttccagttc ctgaatcaga cgattatcgc tcatttcgtc aactcggcga
     8941 ccttctatcc taacataaac atttaagctg ttgtcatgat agctctcttc ttttgtgctt
     9001 aatccggagc aagacaagac aagaatcgat ataaaaacaa ctatgatcac tctatttttt
     9061 gttctctttt tcattttatt atgattttcc gtaattatta agcaattatt cgatcaataa
     9121 taattgcaat atctttctcg tagcatccgt caaataaaat ggtcttataa cgtgaagtct
     9181 cccctttgac aatttttatg gaagacttgg gaattttgaa ggtctttgaa aaaaaagcaa
     9241 tacaagcctt gtttgccttt ccatcaacag gaggtgcaac caatcgcaat ctcaaattac
     9301 catcggcatc cttttccaaa gcattttttg aagaatttgg agtaacttta aaagttagct
     9361 gataacagtt atttttcttt tttaaattca ttccgataag caatcaatat tatattaact
     9421 tcttttttag atacctcgat accggcctct tcaactttat ccataaaatc ttcaatgtca
     9481 agagagggat tacgtaaacg caacttaatt actgcttcaa caatttccgg ttttacaggt
     9541 ttttcaccgg caaaaaatct gtcattttga aaagcaataa tcgcaaatct tatcctctca
     9601 tcttctgtct gcaaatttcg gatatattga ttcgtcgctt tctggtagtc cgaaaaattc
     9661 tttattattc ttttgttctg ctcttcaagc tgcataacct ttacttccag ttcgtcaagc
     9721 ttgtttaaaa gatattctga atccctgcga gcgacatagg cacccggatc ttttaacgcc
     9781 tcttctatct tttttgacag catttcttcg tcaataaaaa cacccgttaa tttcataaat
     9841 atttcgccac tttttaagcg aaaattatag tttaacacat ccgcgctgta atcgaaaacc
     9901 aacccggctg tcatataagg taatagatct gattcattat atttatatgc tcttggtacc
     9961 accaaaattc ttaacgatga tcctttaaca ttaaagatta ttctatcaac agagacatac
    10021 ttcattttat agaacagcat tacaagtttt tctatatctt ctgcacgagc tttattaaca
    10081 cgagaatccc ctttcacaaa aaaaactcgc cctgcagggt cagtaactcg cgcaatatcc
    10141 cgcgaagcac ttctacgtac agaataatct tttgactccc aattatacgg aaacagatta
    10201 gtggttaaaa aaataaccga caataataat atttttttca ttatctctcc cgcaccactt
    10261 ttaacataat tttatcaaaa ggattaatcc ttgcatctgc agacatggta acaacttttc
    10321 cataatatag atccggtccc ttttcaatcg taacagttgc tatttcactc tcatcacttc
    10381 taaaaatata ggcggtatca ccatctctaa tttcataagt atcatcgata tacataatta
    10441 ttcgattctt atttcgtgca tccagaacaa atccattttc accgtctcgc ttaactttcg
    10501 agctgaaagc gtaattataa gcattaatca ttgaattttt tcgccttact cgttcgataa
    10561 gttttgttac aattctatca tatattgcca cggttatttt ggtaaaagat tccatatgaa
    10621 gcagtgatcg aggcactgaa ttagtgtaag gaactgtttt aagttttttt agcaaggcca
    10681 tttcgtccga tacacttctt cgtaattgag aaatatcgtc ttttgttaca gcgcgttctc
    10741 gttgaagatc tttttcaatg ccagataaaa cctctgaagg aattcgcaca ttgattaccc
    10801 ttcgaccaat tatcgcctgt aagcggcttt cattaaagtg tggattataa cgattaacaa
    10861 tctgatccag ttcatcttga tgttttcggg ttgtctcgct tttttgatcc tcatactctt
    10921 tgcgcatctt gttcatctga agattatata gttcatcctt ttccccaaga atcgctccag
    10981 ctgattctac atcagcacca gctttttttc tcaagttttc caattcttct tcggcttttc
    11041 ttatttcttc ttctttattt ttggcaagca ggtcatattt atccctgatt gttccacttt
    11101 tcgaacgaaa ccgtgaatct acagcaacca cttcatcttt agatgaagaa gtagtaacaa
    11161 tagatgattc ctctgccaca ccgacccctg tgtcagatgc ttcttcacta gaagaaggct
    11221 cgtcgtcaaa aatgctagat tctttttctg atcccgtcgg ttcatcgtgc aatacttcat
    11281 catcaaccgc aagcaaagct tctctctgag agcgaaactc cctgtttagc cttgctattt
    11341 catcatcacg cttttcgtaa attgctttaa gttcatattt cagttcatta attcttgttt
    11401 caagttcttt aattctgttt acaacatcaa gagccttgat atctttaaaa tcacttatat
    11461 caactacaag attatcaccc tgcctttgca aaacaactat ccaaacaagc gacgccacgg
    11521 caattgccgc aaagaaaaga agtacaagac cataaagccg aagacttcgg tttttctgtg
    11581 ttttagcgta ttcctcttca attgaatagg taacaactcc tgaatcggga tcatgaaatt
    11641 cagccggtaa aaattctgat ttcgattttt caataagatc cctttttact tgcttttcat
    11701 tgcccatatg cctgtccagt ttttaggagc agctttggat tctgtcctgt ttataaattc
    11761 ccttgctaat ggtaaattga cctgtttaaa ttccttatta gcagataccc acttgccagc
    11821 ataatagagc tttaaggcct tactgaaacg agcgatatct ttcttcagac ttcctgacat
    11881 atattgttca ggtatgggcc aatatatctt tttacctatt gtttttcctt taacctgaac
    11941 aatatcaatt tcaacaaaag ttattcctct ttccgatgtg ttttgcaaaa tatcgttttt
    12001 aacatattct gaacaaatta ctggaacact atatattctt gtaagacctt ccagacgaga
    12061 cgccgagtta acattatctc cgataacggt atttgtcata cgtacataag aacctatatt
    12121 accgtaaaag acttcccctc catccaagcc tacaccaatt tctatcatta acgcgttata
    12181 tacctgctct tcttcccttg taagaccacc tttcaccctt attatagact ctttcttctt
    12241 ggtcatctct aagcgcaacg attcagcgat tgattgtaac tcataggcag ccgcaacagc
    12301 agaaagtgat ttgttctgtg taacaacttc atccatagtg ccaaacatag ccaaaatggc
    12361 atcaccaatt attgatccaa tagctccatc atatcggatg atttccctta tcgccttgtc
    12421 ataatgcatg tttatcagtt taattatatc agtaccaaga gtttccgtta ttgtggtaaa
    12481 acccttaata tctgaaaata gaattgtcag ctctttttgt gtaccttcaa ggcgaattat
    12541 cctttcttta taagccttat atgctatatc cttattggta aaccttttaa aaatttgaag
    12601 aagagtttca attgtcgatg ataacgagtt aaacgccata cccagatagg ttatatcgtc
    12661 gttaggcgcg ttttcaagat gtataagctc taactgctgg tttttaacca ttttcataat
    12721 tgaatttgtt aatatgccaa taaacctaag aaggtgccca attataagaa tccctacaat
    12781 tgttagaaca atggttccga ccagaataac aagtgaaatc atttttatcg tttcaacaga
    12841 ttccttgtta aattcgccat actcttctgc cctgaacaga aaggcatccc attttgagtt
    12901 atatttatat ataccgaaat attcaaaatc accgttattg actgttaaaa aaccctgccc
    12961 ctctttctcc atggattctt tcattttttc aagagctacg ggatcaaatt cgttccagac
    13021 aggtccctca tgggaagcat caagcagtat ttgtccgtta ggtttaacag ccaaaaaaac
    13081 cgcttttcga tttttcatct cgtaaagagc tttgcttttt atattgttta aagaagcttc
    13141 gatattgcca ttaaaagtcg aaatttcata acgggtatta ctataaacat gcagatcttt
    13201 aagatctttg gccagaattt tcttcatcaa cattagctga tgatttctgt tatacattaa
    13261 atttatgaag tttgtactaa ggttggaaag gataataaac ataacaaata ttacaataat
    13321 tttaagacga accggcacaa cacgcgttct gttgacgctt ctaaaaaaaa gacctctgac
    13381 agttcttttc attatgtttt tatccaatat aatgattaag gaattccgtc taacagatac
    13441 ggaaaagttt atggcaaatt attctaaaaa aatccctatt ggattgtatt ttacaatgct
    13501 ttacaaatta tttcaatctt tttttaatta atttaaaaaa actactaaaa ttattacaaa
    13561 tttttactta aatcgtgctt tttttatcta tttccataaa aaaagaacgg ccacatagac
    13621 cgtccttctt tattttttcc ttgtaaacaa aagatatact aaatttcaaa ttcctttatc
    13681 tatcaattgc ttatttcttc ttattgaatc gttatcagga tcaagagtaa gagctttttc
    13741 tatagtctct ttagcaagat ctactttttc aagcctataa taacatatcg ccagattatt
    13801 atatacttcc gcagaattgc tgttaagagc aactgctgtg gaaaatgctg tctcggcatt
    13861 tccatatttt ttttgactca tcaaaacttt tcccaaggca ttatatgcag cgtaatgttc
    13921 agtttccgca gctataatcc gttgataata gtcttgggct gctgcgtaat tgccacgtga
    13981 agccattacc ccaccaaaga cataaagagc tagactattt tttggatctt tatctaaaac
    14041 tgccttcgcg tatgcctcag catgcgaatt tctattagct tccagattca taagagccat
    14101 atcaatcagt ggatccagac tatccggtcg aatctcacgt gtttttataa aatgggtcat
    14161 agctttatca taatttccaa ggcgccagtg acaatgtgca gccaaaaatc ttaacttata
    14221 attttccgat gaaggatcca tatccttctc tattaacaga acgtccgcaa cggcataaat
    14281 agacttcata tattcaccgc gattatagag ctccaatgcc tcatcataac cggccgataa
    14341 agaaaccgtt aaagaaaaga ccattattac tgaaaaaatc attttttgca ttttcatatc
    14401 catacctttc aaatatctaa atctgctata atcttactat ttacaaattt tataaagatc
    14461 catttaaacc atttcaaaaa tttagtcctt tatttatctt ttaaaaagtt cctatactgt
    14521 caaatattta tctatattta tacattatct ttgaaaatat accatatatg actattcctt
    14581 aaaaataaat tctgttctac ggtttttttg gcgtccctct tctgtatcat tcgaagcaac
    14641 aggttttgtc tcaccaaacc caatagagcg caaacgttcg ggactaatac cattttttat
    14701 cataaattcg ctaaccgcgt cagctcgacg ttctgacaat ttctgattat acttatcgtt
    14761 gcctttacta tcagtgtgtc cacgaatttc cagttttaat cccggatttt ttaccattat
    14821 atccagaagt cgattaagta ttggaagtga cgattttctt agatatgcct tgttgtattc
    14881 aaaagtcaca tcctttacca cataagtttt accactttca atcttttcaa gcttaacatc
    14941 tcgttttttt tcatctcgat ccagatcgaa tccttccggt atatatccgt cagaacccac
    15001 aacaacatga acattatcgc catcaggaac tttgattttt ccatccctga caggttgcct
    15061 ctccttgtaa agaggtcttc cttccgaatc ccgctgctct atatcggcat atgcaggcac
    15121 cttgtttcca ccctcatcag taacatcaag atccagatcc cgcatcttaa tttttttgtc
    15181 atcaggagga gaaatttctt tatcagcata acccgttgga tctttttccg tagcagttga
    15241 gtcagaaccg ctttcgggct cgtcataaac cgaacttcca ccggtaacaa ctcgcggata
    15301 agcctcagcg cctatagcgt aaagataatt gcctgcaggc aaaaacaaaa ttccgttaga
    15361 cgcaacaggc tgagaaacag ctccaggaaa aggggtctta aacttccaaa gctcagactt
    15421 gtcactatag ttaatcgcac gaaccgatcc ataagctctg ttgactccac ccttgtcatt
    15481 atatgctata taaaactggt ctctgaccat tacataacct ggagagctct tatcaccaat
    15541 atcaatactg ttttcaattg aaccattttc tggatctgta ataacaagac gcttgttatc
    15601 tattgtaaaa ataacagctc tgtccgtaaa acctggcaaa gaagaaatta ttccaccata
    15661 atcttttttc cacaactctt ttccgttatc aatatcaaga caatagagcg attgtgaaac
    15721 cgggatgtat attcttcttt tatatacaac aggagggaat attttaaacg gttgaggcag
    15781 tcgcttttcc cagatggttt ttcctgttgc accttccaac agtaaaacct tatactccat
    15841 tgtctgataa tccatggatt gaacaattaa ataatcatca tagaaagttg gaaaccctga
    15901 ataactttta acactgctgt ttttccactt ttccttccca tctcgaacat cacgtgacac
    15961 aaacactttt ctggtgccat aatatatctc gttaccggtt aagaccggat cactgtaaat
    16021 tccatccaca aacgcattag tagtagttcc gctaagatct ccttttttaa catccataga
    16081 ggctgatgga ctcatattat aagcagttct ggcccataaa actcttccat tttttttatt
    16141 tatacagtac acagcaacag gatcagttaa atatatataa tcacctttta ttagcggata
    16201 cttgattttg cctgcagacc tggacagttt cttgaactga tccgatattg accttacgtt
    16261 tattttccag atcaactttc cactattttc attcaaacag taaagatttg ctttttgatc
    16321 aataaaatag acaaagccgt cagaaactat cggattgtaa accctatctt cggcctgata
    16381 aagccattta agattaccat taggaaccac gatttcatca ttattccccg taaagaaaat
    16441 attgccctta taaacaggcc aatccgaagc atttaaaacc gaaaacccta ccgcaaaaca
    16501 gagaattgca atcaagatta ctgttttttt tcgcatagcg gctcctcctg gtactgctta
    16561 aggattatcc tatccctgtt tactagtata tcaacaattt ttcggtaatt aattgcaaac
    16621 agttaacagt ttattattac gtcacaaaat taccatgcat tgcgcatagc tttgattttt
    16681 atttagcacg atcggaattt tttatgaaag aaacttggca agcagtgatt cagattaaag
    16741 ctaatatttg gtataatcag tagtgtgggg gcttctcgtt tgaaatttct tcacctagaa
    16801 cttccaggct gttttccaga atcttaaccc tttttttaag agaatctatc tctctatcct
    16861 tttctataac cgtttcgttt agtattctta ccgattcatc aaggtagcta aatttaatct
    16921 caagctcttc tattcgctcg tcactaatca tttataacct tcttgaaata attatctggg
    16981 tactcgcgtt tcagcgtaga ctctatgacc aaacgcgctt tatcagaaaa ttcatcgaca
    17041 ctctgttttg tataactcgt tgacgctatg ggtgaatgta taaacatctt aacttttccc
    17101 gaataaaaga gcctgcttgt ttttggcata attttttcca taccaacaag agtaatcgga
    17161 acaactggta ttcctatatc tattgcaaaa tgaaaagccc cctttttaaa agagcccaaa
    17221 agcacatcat cccgcctagt gccttcagga aaaaaagcaa gacatactcc atccctgatt
    17281 ttttgttttg caatgtttat actttctact gccttgttgg ggtttttacg atcaattgga
    17341 atatttccca atttacggca agccgtacca aagaccggaa catcggtaag ctcttttttc
    17401 attacccagc ggatatccct ttttaatccg gcataaaccg ctccaatatc caaagcactg
    17461 ttatggttaa taacaaaaac atacgattga cctttttcaa gatttttttt accaaatatc
    17521 ttaagacgaa ttggtataaa aaatataagc aaacgggaat gaactatagg aagaacatag
    17581 gcggcctttt cgctgaaaac agaaacaata ataacacaaa tacttataag agctgtcatt
    17641 aacagcaaaa atgggtaaat aattattcga taaagagagt aaagaaattt catttaagct
    17701 tccaacaaga attcatattt atataccata taacagtaac acgttattaa gggcaataaa
    17761 aaaaaccaca ttccttatct atcattaaat cacaactttt gaaattactt gaattattac
    17821 tgcgatttca agctataaag ttttcatgat tagctcttga ctttatttat acataatcag
    17881 aattgtgaat atacagtatc ttcgataatc cggattttct ggaagataca attgatatat
    17941 aatattatcg ctttgaaaac agcgatattt cagggttaat ttgatatgaa tgaaaactac
    18001 gactttaaat caatcgaaac aaaatggata aaaaaatggg acgaatccaa agagtttcgt
    18061 gtaaaagaag atccatcttt cccagctgat aaaagattat atgtacttga tatgtttccc
    18121 tatccatcgg gatccggtct tcatgtgggt caccctgaag gctatacggc aaccgacatt
    18181 tttacccgtt ttaaaaaaat gaacggatac aacgttcttc accccatggg atacgactct
    18241 tttggtcttc ccgccgagaa ttacgcaata aaaaccggta ctcacccggc ggtaacaacc
    18301 agaacaaata ttgaccgttt tcgtgaacag atcaagacat tcggttttgc ctatgactgg
    18361 gatcgcgaga taagcacatg tgaacccgat tattataagt ggactcagtg gatattttta
    18421 cagctgtata agaaaggtct ggcttatgaa agtgaaatgc cgataaactg gtgtcctgat
    18481 tgccttaccg gtcttgcaaa tgaagaagtt aaagagggct gctgtgacag atgcggatcc
    18541 cagatagaga ggcggcgcat acgccagtgg gtattaaaaa taactgaata tgccgaacgt
    18601 ctgcttgccg atcttgacgg tcttgactgg agcgaatcaa taaaagcaat gcagcagaac
    18661 tggattggca gaagcgaagg ggccgaagtc gattttgtaa tcgaaaaaac cggcgaaaaa
    18721 ctccgcgttt acacaacccg ccctgacaca ctttttggcg cgacatatat ggttcttgct
    18781 ccagaacact cagctgtaca acagatcacc tctgctgaac agaaagagca ggtcgataac
    18841 tatataattg aagctgctaa aaaatcagac cttcaacgca ccgagttaaa caaggataag
    18901 accggagtct ttaccggtgc ctatgcaatt aaccctgtta acggctctaa aattccgatc
    18961 tggatttctg attatgttct gataagctac ggtaccggtg caattatggc cgttcccgcg
    19021 cacgacacac gtgactttga atttgccaaa aaatacgata ttcccataat ccaagttgta
    19081 agcttagacg gttcattaaa cgatctggat gaagcaatga cccaggaagg catagctgtc
    19141 aattccggac aatttgatgg gctaaaaaca ttagaattca aaagcaaaat aattgaatgg
    19201 cttgaaaaag agtgtaaagg tcaaaaagca attaattaca agttacgaga ctggatattt
    19261 tctcgtcaaa gatattgggg cgagccaatt cctcttgtac actgtgacaa atgcggtatt
    19321 gtgccggtta aagaagagga tcttccttta acactacctg aggttgattc ttatactcca
    19381 accggaacag gagagtctcc tcttgcagca attaaggagt gggtcaatac aaaatgccct
    19441 gtttgtgacg gtgacgcaaa acgggaaaca aacacaatgc cccagtgggc tggaagctgc
    19501 tggtactacc tccgttatat ggatcctgca aacagcaaag ctcttgttga tcccgacatt
    19561 cttgactact ggggaagtgt agatctttac gtaggcggtg ctgagcacgc cgttcttcac
    19621 ctgctgtaca gccgtttctg gcacaaagtg cttttagact gtggagttgt ctcttttaaa
    19681 gagccttttc aaaaattacg aaaccagggt atgattcttg gtgagaacaa tgaaaaaatg
    19741 tccaaatcac gcggaaacgt tataaaccca gatgatgtta ttgccgagtt tggtgccgac
    19801 tcgcttcgaa tgtatgagat gtttatgggt cctcttgctg ccgataagcc ctggaacacg
    19861 agcagcatct ttggagttaa gcgttttctg gacaaggtat ggcggctgca ggacaaaata
    19921 tcttctgact cgcaactttc acctgaagca cttaaaatgg ttcataaaac aattaaaagt
    19981 gttacttata aaatcgataa tcttgatttt aatacagcca taagccagtt aatgatatta
    20041 ctaaacacat tacaaaaaga accagcggtt acacgagaga cataccagac tttgttaacc
    20101 ttactacacc cttttgctcc atttatcacg gaagaactct gggaacagta cggaaacgga
    20161 aaatcaatgc ttaaaaccaa ctggccatct ttcgatccag atctcgccgt tgacgacgtt
    20221 attactgttg tatttcaggt aaacgggaaa gtccgctcac gcgaagaact atcggcagac
    20281 tcaagcaagg aagagatgct tgaaattgca aaaaataatg ataaaattaa acaatatctt
    20341 gacggaaaaa caattataaa ggaaattgta gttcttggaa agcttgtaaa cttggttgtt
    20401 aagtaaacac cgctatcatt agtacttgtt tatattatca cattaacaat tctaaaatat
    20461 atgtataaat ttcttcggcg ttacagatag atcctctatc tgtaacgttt ttttaatctt
    20521 tattcacaac ttctcttatt atttttttca gttttccgac aatattaata actttttatg
    20581 aatattattt gacaagtatt atgtcacatt ccataattag taaaaaacag atgcaggggt
    20641 tacttcaatg aaaactaaaa tggttcctac agaaatatat tcacgcgtag ttggttatta
    20701 ccgcccagta aaccagtgga ataaaggcaa acagtccgaa tttgctgata gaaaagaata
    20761 cgatgttaac accctaaccc ttgcctctga acaaactgaa aaagaagcaa tagctgttta
    20821 ataattcaat agcggaacgt ctttaaaaaa tgttccgctt attcacacaa ctctctatta
    20881 attcttttat acaaatttgt aatatacttt gatgctctaa aagcagattt ttttatgctt
    20941 tttcctttaa ttatattgca tgcggtagcc gtagccatcg cgcagccagt tccatgtgta
    21001 tattgcaaat tccaacgcgc tttctttagc gtataaattt cttcgcctac gagatattct
    21061 tttatatact tgctggcagt ttcttcatga ccccctttta tataaaacaa cggcttccct
    21121 ttatatttgt cccctattat tttaactgtt tcaatcactg ttaaaacttc catctgtgtt
    21181 aatattttta actcttcaag gtttggagta accagacaag acagaggcaa aagttcattt
    21241 gccaaaaaca attgcatctc tgcactgtaa aagggtctac cttcagtagg tgaaaatact
    21301 gggtctatca ctactggtaa ttccggattt tttttaatat aatgccaaac catcttcatc
    21361 tgatcaatag acaaaagtgc accaatttta agaccaccgc atttaaagga taaaccacat
    21421 tctagctgtt tttcaaaaat tttattactt acaggctcta cccctattac tccatcaaaa
    21481 gtctgggcgg taactccact aactgttgtt accggccaga cattatgatc catagctact
    21541 ctcacatcct gttgggtacc agccccgcag gaagaatcag tcgccgaaat tatatgaaga
    21601 aatttactgt ttaagcgcac agagagaacc gcacattgta caataatctt tttcgctgga
    21661 ctctgactct tctcgacgtt ttcgaggaag ttcaggatca atcgagcagg catacatttt
    21721 ttcccaatca agtttcttac ggtactctgt cattagcctg tttcgatcat atgcacccgg
    21781 aactcctttt gctacatcac ccgcctgtgc cgcaataacc gaggctacta ctccctcacg
    21841 aacatcatct tcatctggca aggtaagatg ttcggccggt gtaacgtaac aaagaaaatc
    21901 agccccgtgc caagccgcaa ccgccccacc aattgccgcc gtaatatgat cgtaacccgg
    21961 tgcgcaatcg attacaagcg gtccaagaac ataaaatggt gcctctttgc atactttctt
    22021 ctggatctcc atattaaatg gtatatcctg tattggaaca tgccccggtc cttcgaccat
    22081 tacctgaaca tcacgctctc ggcacctatc aacaagacgg cccaaagccg caagctccgt
    22141 aagttgaagc ggatcagtcg catctggcat gccaccagga cgcaagccgt cgcccagact
    22201 gatcgttaca tcatatttat aggctatatc caatagtcta tcaaattgag tataaagagg
    22261 attatccttt ccggtctctc tcatccagcg cctagtcagt gctccaccac ggcttactat
    22321 accagctgcc cgtttatcag gatcatgact ggaaaaagtt tcacaattaa caccgcagtg
    22381 aactgtcata aaatctacac cctcttttgc ctgttgctct attgcaccga aaagacgatc
    22441 accatcaata tccttgatag aacccccatc tcttacggtc tcggcgaaca ctgcatatag
    22501 tggaactgtc cccaccatta ttggagaatt ttcaagaacc ttgcgtcgca tgccattaag
    22561 gtctccacca attgagagat ccataacgct atgtgctcca tattttatcg ctgtctcaag
    22621 cttcttcatc tcgttagcaa cattattgca atcaggtgat gtaccaatgt tggcattcac
    22681 cttagttttt aatcccttgc caacaccttc cgcgctaaaa gtacgattac agttttttgg
    22741 tataacgatc tttccggcaa taattccttc actaataaat tcaatcgatc gctgttcctt
    22801 tcttgcgacc tcttctatat caggagaaat atcccctttt tttattctgc ttatctgtgt
    22861 cattttttac ctcgcaagaa gagacgaata aaaagaaatc tcttcttcaa tatttgcaga
    22921 cttaacaagc cctttaatca tggcaatatt tttggctcct gtttctaaaa cttcactaat
    22981 attatcccga tttattcctc cgattgccac aaccggacaa tcagataaat caagcacctg
    23041 ttttagctga tcacacccta ctggagcatc aggatttttt tttgcaaaag tagggtatac
    23101 tgggccaaat ccaatataat ccggtttcaa ctttactgct tcttttgcct gttccagcga
    23161 atgcgtagat agcccacaaa caagattacg atttccagca acttcccgcg cgttaacaaa
    23221 aggaatatca tcctggccaa gatgataacc atcggcaccg gatgcaatag ccagatcaac
    23281 tcgatcattg attactactt ttgtagaact acctttggta atagagagca ttagctctaa
    23341 agttttgaga agctccttgt cacaaagctc tttttctcgt agttgaagca ttccaacatt
    23401 atactttaca caaatttctg ttattttcga aacgggataa tcaggatcag taatgattac
    23461 ataaagacca aaatcttcca tattaaagtt tttccataat taaattggct acttttcgtc
    23521 ccgacataag catgccgcca aagataggcc ccattctgaa acttccgctt actccgttag
    23581 ccgacatacc tgatacgtac attcccggat agacttcctt tgtattttca atagttgttc
    23641 tttcgccttc aacaacagaa agtgaacact cacccattac tccacctgtc ggtgtattaa
    23701 gctttatgtc atttttacgt gctaatgtag aaacgatttc gcaaggatgg cctgtaccat
    23761 ctagaacagc tttggctctg actacaagag gatcaacatg cattccaatg tttactacag
    23821 gagtccaatt aataacaaca ccgttaactt ttctggtctc actgtgaaca acaacgtctt
    23881 caaccttgac accgttaaaa attaccacgc cgtttttaac ggctctgttt attaaagccg
    23941 aagttgcttc tactgaatcc agcataacat tgttatcatc atattcagta tatctgatat
    24001 caaactcctc tataatatct atagcctctt tttgcaccat aatctgatta aagagcattg
    24061 ctccacccca catgccgcca cccggcgcca attttgattc atacaaagcg acctttttcc
    24121 cggcttttgc caaatagtag gcggcggtaa gaccagatgg tcctcctcca acaatcgcaa
    24181 catcaatatc cagattgtct tcaagctttg caaaaaatga gcggataata ccgcgtgaaa
    24241 ctgttacttc catattgtat cctcccggaa aattattgga ggataatgct gtgataaaga
    24301 aagaggtaaa aaagagggtt tctttcccta cgctggcatt atccagatca ggttatacgg
    24361 gtttaatctc agccccaaag gagcaccccg ggtaaaagca gtagctttca cctgatacaa
    24421 tgattattaa ttcctgtttt cagtcaagca atatcataat tcaatttact accggtttta
    24481 ttacatatta cttgaaagca ttagtaaact actatagctt caattttatt tgcatttaat
    24541 tttgattttg attttaaatt catattatgt ttagcatacg gttgtttctg tattctgctt
    24601 aaaatcaata ttatattttc cccgcaaaat ttttttaggt ttgataaaag aatcagcgcc
    24661 aaactgtcgc ataatcatag ttatgacgaa tagtatcaat tatttaaaac tgtcaaaagt
    24721 actactctaa atatgctatt tcacaattat agattcccga accctggtta aaaatgcttg
    24781 atgaacagtc cttttaacag tactatacat aataccatta aaaggattat aaatatgaca
    24841 caaataaccg caaacaaatt aaaaaccaga ggggtctcaa tactaaacga agaaacctct
    24901 gatggaaatg aagtaattat aactgtaagg ggagagaata aattcgttgt tattcctatc
    24961 gaaaaatata attatttaag agaatgtgaa ttagaagcag ctttgcttga aactaaaaag
    25021 aacattaaag acggtaaata ttttatctat cagtccgcaa acgtcacacc ccgtgcagca
    25081 atataaacac cgacagatca aaatacagtt attttcttta atattttcat tgaccagctg
    25141 ttaatattat aaattatttt gatcattgtt ttatttttat agctatccac acacaagatt
    25201 gctttttttt gacttgatga tattttaatc aaattttcag aatcacacat ttaacaaact
    25261 aacacattac ttatttaaca agtacaaatt tatggttagt aaaacaatgg gttgtattat
    25321 cagtatttct aatgacggct ggccatgttt tatatacaac ttttaaataa taactaattt
    25381 tgcaatgaag aagtactgtt ttataatcca atgttacaaa tatgattata ttacaaggta
    25441 attaattact tgactaacaa actaattaac ataaccatat tactatggtt ggattttatg
    25501 aaacttgaaa acgattttac agatatgatt tcttacctga ttgagttcga ctgccgcttt
    25561 attgtagtcg gtggcttcgc attagcgttt aacggattta ttcgcaatac tggcgatatt
    25621 gattttttcg tagaaccaac tataaaaaac tctgaaaaag tctttaatgc attaaaatct
    25681 ttcggtgctc caattcagga tataaatcca catgacttta cccaaaaagg aaccatatat
    25741 cagatcggac ttccaccaat acgaatcgat atcattaatt caatagacgg tgtttctttc
    25801 gaagaagcat atgcaacaaa gaaaataaaa aaaattgacg gattaaaagt accttatcta
    25861 tccattgaac ttcttaaaaa aaataaaact gctagcggcc gctcaaaaga tttatttgat
    25921 atagaggaac ttaacaagct atgaatagtg caaatgcaaa agagaagcgt aaacaggaac
    25981 gattaaaaaa atggtcgtct tgtctaatac gtaacaacga agttattgcc ggcaaagaga
    26041 ttacgaataa taacgaacct gatattgacc gctgtctctc acaacttcac gaactacgca
    26101 gaatggcttg gcctgaactt gatgaagatt accgtatgga aaagagcatt gcaaaaataa
    26161 tccggccttc ttaacagaat attaattatt ctgtaaactc ggaaactaat tacgcttcca
    26221 tctgcagatt atttatatgt ttatctttct tataaaacta tacaaatatc tctcatttta
    26281 gaaaagcttc tatcgcaatg caccagaata tgaaactgaa taatccgatt ataaaagtaa
    26341 agaaaaatta acaatcaaat atcataactg atattatttt tcagacgagc cttcactgtt
    26401 ctatccacaa gatcctgtat cgttacactc tcaagaaagt ctatcataca ctctgataat
    26461 tccttccaga gatctcttgt aacacatata ttttgcctgt tgcaatcaac ggtgtcatca
    26521 acacagtcaa caaggctcag acctccttca agcttttcga caacttcacg cagtgtcagt
    26581 aaccgagctt cttttacaag tcggtatcca cctgttttgc cacgttcaga ggatatgata
    26641 ccggcacctt taagttgaat taccagctgg ccaagatatt tttctgagat atcctggctt
    26701 tgcgctatgg agtttaactg taccgaaccc ttgtcctgat taagtccaag ttcaattaga
    26761 aggcgtactc cataacgtga gcgcgtggat aatttcattt gctatcctca aaaagccaag
    26821 tagaaagata acgttcacca gtatctggta acaacacaac tactcttttg tctttattct
    26881 ccggcctttt tgaaacttca agagctaccc ataatgccgc cccactggaa ataccaacaa
    26941 gaatgccctc tcgttttgcg gcttcacgcg ctatatcccc tgcatctgtc tgcttaacct
    27001 gaatagtttc atcgattaca tcaacgttaa gattttttgg gataaatcct gctccgattc
    27061 cctgaagttt atgtgggcct ggttttcctc cagaaattac tggcgattca attggctcta
    27121 ctgcgattat ttttactgca ctgttttttt gttttagaaa ttcaccaaca ccagtaattg
    27181 ttcctcccgt accgacacca gctacaaaga tatcgacttt accgtcggta tcactccaaa
    27241 tctcttcggc tgttgtttca cgatgaacct gcggattagc aggattatca aactgctgag
    27301 gtatataaga atttttattc tcacgagcaa gttcttctgc tttctcgatt gcacctttca
    27361 tacctttcgc gccttcggtt aaaactagct cggcaccgaa tatctttaat atatgtctac
    27421 gttcggcact catggtgtcc ggcatagtca gaatcaggcg ataaccctta acagcacaga
    27481 caaaagcaag gcctattcca gtattaccac ttgttggttc aataatgact gtatcactgt
    27541 taattttacc ctcttgctct gctgattcaa tcatcgactt tgcaatgcgt tctttaacac
    27601 acgataccgg attaaaggat tcaagcttta caacaacttc ggcataaccc ttcttgttca
    27661 ttttattaat tctcacaagt ggcgttttac caatagtttc agtgatatcg ttgtaaattt
    27721 tcatattacc tccattttct gacaataatt ttaatatcta tcagtatata tattcttgtc
    27781 aaccttatta tcactatttc tatagtttta gtagtattta caatttttgt ttaatttgat
    27841 tattaaaaat gctgaggaag gtatacacaa taaagggcaa atccgaaaac gacattgcgc
    27901 ctatttgcgg attaagtatt ggcctttcat tttaacaact caaaataatt acggttttac
    27961 attatttctg tcataccatt ttttccataa atcaaaagcc gcgcggctgt tgcccgactc
    28021 atcgaataat ccagtatctc gccaaacaat cccgattgga tctttattaa gtgcgccttc
    28081 ccaaagagca tcataatcat gtatcgaaaa ccagattaca aataatgact catatatatt
    28141 tgattcatta agaaggctag atagaaattt ttcctgtttt tctggtgttc cattaaaatc
    28201 aattattggt tctgttattg tctgtcaata ttgtgccgga tagctgcttt ccgaaacggc
    28261 aacaggttta gtcgttattt cgaataattt ttttaaataa tcatccggaa atttttccgc
    28321 agttaaggca ctcataaaag gatgaaccga aaaagctata aaatcggagg aggcttcaag
    28381 ctgttttaat ccaataagtt gctcttctaa atcatcttct ggcgaccaat caggaaagta
    28441 gggcacacaa aatacagaaa cacctgttgg caaatctggg tacttctttt taatctgggt
    28501 atacacataa ttatgcaact ctatatatgg ctcccataaa acaggactat tgcgaataag
    28561 cagattaacc tcgataccga taatcaggta atccggttta aataattcaa tcattctttc
    28621 tgtatatttt aaatatgccg tttttacctc attgctgttt aaagcatagt cactccaagg
    28681 tgaaggcaaa accataccct cagaaattcc tctgtatagc gccataccat cacgtcccat
    28741 atttaagggt gaaaccgaca gcaatatttt tttgtctgct tggtatcgct caacgcgcga
    28801 attaatttct gtctgaaatt catcagcaaa cgattccccc gcatatgact caggccaggg
    28861 aacgccttct tccatatgat gcgagatgat atcgccattt ttattaattg cgtcgtagat
    28921 ccactctact gcttcaaccg tggaatcata aggccaggga gtaaatccca ttaaaaaaga
    28981 acgtttattg ttattatcat caacatcatt tgtacaagct acaaagaaaa acagcaatgc
    29041 caagattcta taagaatttt tcataaacct gtcctttgcg cgtttaacat tattttcgtc
    29101 atatattcat cgtaaatatt tttcaactgt tcaacttcat gctcgctaac ctttagcggc
    29161 tgtgctttat taaaacacat tcccttttcg ctcataaaat ctatacaaaa gataagggta
    29221 tacagatcaa gagccgcctg ttccgattga tttaaaccac gatattcaag ccagtaatct
    29281 atatatgtta aactataatt tgaatttaat agtgacattt tagttaaagc tatggtataa
    29341 agttcatctc caaagcagat ataatcaaaa tctataatgc cagaaaactt cccatcattt
    29401 ataagtacat tttttgtcgt ggcgtcatca aaaaacgcaa gaggaggaat tgtttcaaaa
    29461 tatgaaaaaa aatcaggcaa cagtttttca actttatcag catatgactc tgagaaatca
    29521 ccgttttctt ttattctgct tcttgatcta tttatatgct ctttaattac atccagccag
    29581 caatccttct tgttcggatc cgtcatatta gacaaataac catatccatc tgcttgccct
    29641 aacttcttta cctctctctg tatattaacc agattttgtg ctatctcttt tttctcagat
    29701 tcatttaacc gctgataaac attaccaaga tcatctcctt cgatataagt ataaattaca
    29761 aatggagtct gacaattcag atccatggtt acaatatccg gtatctggat atttaatgtt
    29821 ttaagtctac caatccaata tactgaattt ttcaatgatt gcaaattatc ctgtttgcat
    29881 attttcacaa catacttatc gttttctgtt tgcagataaa acgaataatt tctcaggccg
    29941 gttgtaaatc tgtaacactc taacggatca gtattcagat attttttaca aatttctttt
    30001 gcaatttgta tgttcacaga cgacctcatg cattaagaat tatgaattat gaattatgga
    30061 tataattaac gaaaccccaa aaaagcccct ggtttttccg aaacgcatat gaatttattg
    30121 ttattttttt cccgttgtat ttaaattcaa aaaaatatcg tgatgatatt gatcccaatt
    30181 tcttttcgaa acacattgag taaatatatt tttgacttcg atatcttttt cgaacttcta
    30241 tatattcttt atcaatcaaa tatactgtaa aacaggtctt gtgctctaca ggcccgtttt
    30301 caatcgcatc gatacccttt tgttttatta tttcaaaatt attttcaatt atagtctttg
    30361 caagaaatct tgtttttttc ccaattaatg gtatctcaat cttccgatta ttataaaaat
    30421 ataattttaa agctgatgaa gactctgctt cataaacaaa ccaccctgta agttcactca
    30481 gtaaatattc ctttatatca acaaacggct tttttactgt aactttatta cccagcaaat
    30541 aggaatattc accagaaagc aaccaaaaac aaattaagaa aattatggtc attgcaatat
    30601 aaaaacataa agcaaatttc tgctcttcta ctaaacccca aaaaccactc aatatcaagt
    30661 atattggcca aatccatatc agagataaga atagacatag gcctctaatt acaattgatg
    30721 gttttgcaac cagagtttta tcttccattt cgaaccttta cctttttttt ttattatcga
    30781 ttgggtgctt caattaatta ctggtatcaa aactgtacat tttgaatttt acaagtcatt
    30841 aacttttaaa tattagcaaa ctttatt
//
LOCUS       DAOTGZ010000009        30454 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_3064
            NODE_1685_length_30454_cov_4.931248, whole genome shotgun sequence.
ACCESSION   DAOTGZ010000009 DAOTGZ010000000
VERSION     DAOTGZ010000009.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868088
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 30454)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 30454)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:22:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,785
            CDSs (total)                      :: 2,751
            Genes (coding)                    :: 2,732
            CDSs (with protein)               :: 2,732
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 12 of 19
            Pseudo Genes (internal stop)      :: 5 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..30454
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1685_length_30454_cov_4.931248"
                     /isolate="AMR_MDS_3064"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..325)
                     /locus_tag="P5123_01435"
     CDS             complement(<1..325)
                     /locus_tag="P5123_01435"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRX14957.1"
                     /translation="MNTKWIIALIFLNLWTHLLSEPYDIIGRWEIRDSDDTNSISVIY
                     EFTKSSVTIYSVINKSVKAKSEYQYTIINNLLYIYRYKKDNNADIIKKNYYIIKEATK
                     DLIKLE"
     gene            647..1144
                     /locus_tag="P5123_01440"
     CDS             647..1144
                     /locus_tag="P5123_01440"
                     /inference="COORDINATES: protein motif:HMM:NF016985.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar protein FlgN"
                     /protein_id="HRX14958.1"
                     /translation="MELADKLVIVLSEQAALYRDMESIEQQKKEIIIARDGKELERLV
                     SRQEKIITDIDILEKRRIKIMDEISFSSGRREPVITARQAAVCSDRRSGELIIRYANE
                     LRNIVKRIKKLSDVNERCMKDNMEFFTLIIDSLRQTVRGEEGYSASGVAPKRSAESAL
                     FNLTV"
     gene            1157..3064
                     /gene="flgK"
                     /locus_tag="P5123_01445"
     CDS             1157..3064
                     /gene="flgK"
                     /locus_tag="P5123_01445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017852413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009424 - bacterial-type flagellum hook
                     [Evidence IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook-associated protein FlgK"
                     /protein_id="HRX14959.1"
                     /translation="MSSTFMGLEIGKRGLSSHQQALQVTGHNISNAENKEYSRQRVVI
                     TSADPIYEPAFNRAERAGQIGQGSTVARIERIRDAFVDDRIVTEKNSLGYWQTKDKYV
                     YQIENIYNEPSDNSIRTKSDEFWKSWQELSKYPEQRSTREVVKEKGVALSNEINHVSR
                     QLLDLQRDANREVALRVEQINEYGTTIRDLNMRILKAESLGDSPNDLLDRRDALIEKL
                     SQLVDVSVGRSDEDELIVYIGSENFVQGEVLHKIAVKEDPDNQSFYKVVWQKTEADVT
                     LKSGELQALLDVRDKVLQQNIDDIDSMAMNMMDLTNEVHRDGFGKNGRTNIDFFRTIS
                     LSENIEGNADLNNDGEDDVSAIFKVAGKNRIDASAAIGINGTLLFRKNDGTETLLPVD
                     YVSTDTANSVIQKINNAKLGVVAYMTHDGNFALKATAALDNPSRNFMIRHLEDSGQFL
                     VGLTGVLQNSGPDGAYEYTELNAVRNLAPEGDHITLTPRKNPSVYMAVSKEIIDDVDN
                     IASAQGIDSDGSGDYNLSNGVGDGQNAMAIASIRHKDAMVQTSSTFDDFYISLISRIG
                     SEGEESRDRVNNQEILLSNLGNLRESISGINLDEEMANMVAFQHGYNASARMISTFDK
                     MLETIIGLGGR"
     gene            3064..4326
                     /locus_tag="P5123_01450"
     CDS             3064..4326
                     /locus_tag="P5123_01450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004781212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook-associated protein 3"
                     /protein_id="HRX14960.1"
                     /translation="MYRVTNKMITNTMTHNLQRHKREMDQAQNSLATGKKVRTPRDNP
                     IATTNQMQYQTRLNEVNQYIENISESKSFLEQTDSSLQSMVRIFQRIRVLAVQGAHGI
                     YSSFERKEAAATEINQLLEELVAISNSKDATGRSLFGGYETGSTENPNPFVPIYMTLT
                     AGNQGDAMTGVEYRGDMGRLTREVAKGEYMDISAPGSYAMWATNQVVTSNFDASEYSS
                     NANQKIRIDGVELNIAPGDNIDIIIDKINNAGLSVRASKGGRNNLIMETTAPHKMWLE
                     DVGDGQILKDIGMINPLKDQPPNNYAPTATVDGHSIFDMVIQLRDDLVRGDQELVGGR
                     DLGLIDMGLENILKHLAETGAKQNRIEQLATRSEYMKTNVTEILAKTEGIDYTEEIMN
                     FKWIESVHQYALSVGAKTIKPTLMDFLR"
     gene            4382..4828
                     /gene="fliW"
                     /locus_tag="P5123_01455"
     CDS             4382..4828
                     /gene="fliW"
                     /locus_tag="P5123_01455"
                     /inference="COORDINATES: protein motif:HMM:NF009793.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006417 - regulation of translation
                     [Evidence IEA];
                     GO_process: GO:0044780 - bacterial-type flagellum assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar assembly protein FliW"
                     /protein_id="HRX14961.1"
                     /translation="MTINTKQFGSIDIDESEIISFPDGLFGFESLKKFTLIEEPDSPM
                     MWLQSCSESDLAFVILRVVDFMKEYDLSISQSDLESVGTEDPSELDVFAIVTIPLENP
                     SEMTANLMGPVIINMKDKKGRQVISLCDKYKTKHRVLDELQAKTGA"
     gene            4832..5068
                     /gene="csrA"
                     /locus_tag="P5123_01460"
     CDS             4832..5068
                     /gene="csrA"
                     /locus_tag="P5123_01460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010573184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_process: GO:0006109 - regulation of carbohydrate
                     metabolic process [Evidence IEA];
                     GO_process: GO:0006402 - mRNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbon storage regulator CsrA"
                     /protein_id="HRX14962.1"
                     /translation="MLVLARRTNESIMIGDDIEIVVIDIKGDQVKLGIKAPKKVTVHR
                     KEIFDEIKQENIAAISTPFKPDTLRDLSDFFKKK"
     gene            5273..7207
                     /locus_tag="P5123_01465"
     CDS             5273..7207
                     /locus_tag="P5123_01465"
                     /inference="COORDINATES: protein motif:HMM:NF016611.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HRX14963.1"
                     /translation="MKKILLFAVVLSATFLVAQPKNARYYNKEGWNYLEKRDPVKAMA
                     SFKSAVSQNKDYKEALTGLGFSYLMRGAADDAFEIFNNLSKKYPEDEKILLGRAASLG
                     EMGRYEEALNDYARVTEIRGENSEALYGTALIYYRMGKNVQAKRTIDESLRVNPYHYD
                     TLILDARIHLDEQRLDQAEGIIQKAINVRQEFPDAYVQYGKLLLNRYKKESNPAFLSD
                     SREEFNRAISINPFDPESIRMAGIIDLINDDASSARDYFSKALDINGNDFATLYNLGY
                     SYELLANTDKAADFYQRATNLAPDDVFMNSKMQTFLIENNFAFGNPQRVQYARLNYSE
                     YKKNRSRNMSDYALMHLRYCVVLNPMDQDARKELAAFYNIAGFNRLYINELKVIQRLH
                     PDNSNREALNLAVIRRRDRLYNRSGYTLDMPPRNVVKVLVLPFYDRSGNFSMFDKGDV
                     FSDNLVFALSQFGRIQILDAAERKIIIQKMNNYDNISEALAKISDEDIDFIVHGSFNA
                     GKTYIDSEYNIVSNHTGAVHKTFELHESGESALNRINFRAARDIADAVPLRGKILKVQ
                     NGEPLVNLGLYDGIEKGANLFVYSSSGNTAKGRRIMLKVKETDAFLSTAAVSKSSEAD
                     MLVTGMDVYELKIRRARLIK"
     gene            complement(7331..8209)
                     /locus_tag="P5123_01470"
     CDS             complement(7331..8209)
                     /locus_tag="P5123_01470"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRX14964.1"
                     /translation="MFAQKFEKWEIFEQKGIIQYEEQMYDFADEHLNRALEMNPKAYR
                     SAKYLGFLYHYKEDYNRALTFFLLSLDTNPKQDDAHYMVGEIYDRKMKTAEALSHFKK
                     SIEINDNYYKSHLGCARTESILGNTKQSDYHFNRAFEINREKSMNLFSQARDKRTKKA
                     NKSSLALLEKALTLNPAHKEIYFELSSVYRAEKEFESAIRTLERLKYYLPHETKIYVM
                     IANIYYSERVLHRRNNDYQAALFNIERALEFDPDNLQYLELAQELYKLNGMNKEYEET
                     SKKVLRLTLEQFEDTK"
     gene            8302..8374
                     /locus_tag="P5123_01475"
     tRNA            8302..8374
                     /locus_tag="P5123_01475"
                     /product="tRNA-Phe"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:8335..8337,aa:Phe,seq:gaa)
     gene            8643..9905
                     /gene="serS"
                     /locus_tag="P5123_01480"
     CDS             8643..9905
                     /gene="serS"
                     /locus_tag="P5123_01480"
                     /EC_number="6.1.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013777373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004828 - serine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006434 - seryl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine--tRNA ligase"
                     /protein_id="HRX14965.1"
                     /translation="MLDPKFVRENYDYIEKMLKLRKMEGVCDINELKSLDDKRRDLSS
                     EMNSLREERNSVSKSIGIRKQKGEACDDLMEHVQTINDKIEAITDSLNKYDTAYNDIM
                     LSIPNRLHESVPEGTDESFNKVVREWGEKPAFDFEPKPHYDLGADLKILDFERGVKLA
                     GARFYVYRGLAARLERALISLMLDTHTNEHGYTEVFAPFLVNDESMIGTGQYPKFKDE
                     YYRLDRDNLSLIPTAEVTLTNLYRDEILTKEDLPLYVAGQSSCFRREAGSAGRDTRGL
                     IRVHQFQKVEMVKFVDPESSFDELEKLVANAEAILQKLGLHYRVLLLCSGDTSASSCK
                     TYDLEVWMPGLNRYVEISSCSNFLDYQARRAMIRCKSKDGGKPEYLHTLNGSGVAAGR
                     ALAAVMENYQHKDGSIGVPEALKKYLER"
     gene            complement(9920..10240)
                     /locus_tag="P5123_01485"
     CDS             complement(9920..10240)
                     /locus_tag="P5123_01485"
                     /inference="COORDINATES: protein motif:HMM:TIGR00377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043856 - anti-sigma factor antagonist
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="STAS domain-containing protein"
                     /protein_id="HRX14966.1"
                     /translation="MEISNRTNNQYQIFDIKGNIAFEETQEMEDYIYTNISVDKNKTI
                     INLKEVPYLNSSALGAFVRILQTLKNKKMDLFIMNINSDIDNLFKITGVNKYFNFIKE
                     SDIN"
     gene            10826..11116
                     /locus_tag="P5123_01490"
     CDS             10826..11116
                     /locus_tag="P5123_01490"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRX14967.1"
                     /translation="MSSKAVVTKSNRKGRAKRKVNLIRLHTAMNKLASDANDKEIVKR
                     FKILIDTSDEDLSKARVDEILKTPQKVEINSFHESLQPYVKHFLFLAKKNSK"
     gene            11125..12342
                     /locus_tag="P5123_01495"
     CDS             11125..12342
                     /locus_tag="P5123_01495"
                     /EC_number="5.4.2.12"
                     /inference="COORDINATES: protein motif:HMM:NF003242.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cofactor-independent phosphoglycerate mutase"
                     /protein_id="HRX14968.1"
                     /translation="MSSKYLVIVGDGMADLPHEQLNGQTVIEASFMPNSDKIASFVCG
                     LTSTVPDGMNPGSDTANLSIFSYNPAQYYTGRAPLEALNLGIDLGPNDMAFRMNFVSI
                     VQNRMDDFTADHIDSQYGKLIVDQLKKLNHPDIEFYAGVSYRNIMVWRNYPYDEMPLT
                     VPPHDIQTMDVSSYLASGSGADLLREIACEARLVIADVVSEDHGLGGNPTDIWLWGAG
                     KKPALKRFSEKYSISGVTVSAVDLIHGIGRAAGLNPVTVDGATGYLDTNYKGKVDAAL
                     NAFETHDFVYLHIEAPDETGHEGSLEKKRQAVEDFDLKVVGPMLEGMKKFNDYTILLM
                     PDHPTPVSVRTHTSDPVPFAFISTEPQFVDVHRKYSASSYSEKEAKNTGLFVKDASML
                     LDIIIKKKISSIM"
     gene            12351..12911
                     /locus_tag="P5123_01500"
     CDS             12351..12911
                     /locus_tag="P5123_01500"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRX14969.1"
                     /translation="MSSKRANLFIVVLLILLIECTGVAPEHRDEKSNENVVPSDDYII
                     DEIEEFTFDDVEEQSGDPRPEVTSLPGAAFRIHLSSFSYRVEQLRYFNRLSLVRNDNR
                     EGGLRSIAARYNRTDSVRSAHVLISIFPSTGTLKQYRLIKSTYIKELDALIMKDLSTM
                     RFSIVRNKIPSVLYIAYTIQLSDQRR"
     gene            complement(12988..13665)
                     /gene="rpe"
                     /locus_tag="P5123_01505"
     CDS             complement(12988..13665)
                     /gene="rpe"
                     /locus_tag="P5123_01505"
                     /EC_number="5.1.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004246629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004750 - D-ribulose-phosphate 3-epimerase
                     activity [Evidence IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribulose-phosphate 3-epimerase"
                     /protein_id="HRX14970.1"
                     /translation="MSPFLIAPSILAADFARLGEDTDRVIKAGADMIHFDVMDHHYVP
                     NLTFGSMVCKALREYGITAPIDTHLMTTPVDDLIINFAKAGSTYISIHPEATHHVDRS
                     LELIHSCGCQAGIVLNPATPVEWLTYTIDKVDMILVMSVNPGFGGQKLIPSTFAKIKA
                     IREIIDSSGRDIRLEVDGGITEENIGSLAKAGADTFVSGSAVFHSKDCSATINKMRME
                     IDKAIKS"
     gene            13891..14760
                     /locus_tag="P5123_01510"
     CDS             13891..14760
                     /locus_tag="P5123_01510"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRX14971.1"
                     /translation="MKSVDINYFTDNQAGEAATIQLKKLGLNVNRADIDKISSISPDP
                     DKVTLIVADIDSMPVSDIIEKFNSLPKLHNIVKYIVLDSSLIVHAGDYRGSAMHTEFI
                     EKPYNRYEFALLIEKSIIVEKYRQIMKQLSNEYEKRIEQYENIFHISNSGVIATDQDK
                     KAFEKILDFEKNLIKKQEELNEKLHDFSSFRQKDLLETQNLLFANTMLDRLREKELMD
                     ANELIHAQERVLEYSSSRLGDNLKLVEAQEKVVELSRDEAISLHHTIENLIKEKKYLI
                     EENQKLKDLLQKK"
     gene            14898..15176
                     /locus_tag="P5123_01515"
     CDS             14898..15176
                     /locus_tag="P5123_01515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010515454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRX14972.1"
                     /translation="MEWEKLLRNSVKDGTIRELYLRKIPVLKNCDNWNKIEPIGYIDT
                     PMKYTHYKGILVMYQDRIFFVTSKTMEALSEFVKWNIRNKIRVIPEKK"
     gene            complement(15178..16929)
                     /locus_tag="P5123_01520"
     CDS             complement(15178..16929)
                     /locus_tag="P5123_01520"
                     /inference="COORDINATES: protein motif:HMM:NF025011.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helicase-associated domain-containing protein"
                     /protein_id="HRX14973.1"
                     /translation="MDPAKIKELQKIIGASGSTSKSAIPAALMKLGGINSVLSSLRPD
                     ELQIFSFISTDEEGRTFSEIEKATGFDIATIESVCDNLKDKLLIYIFKNRKHLNNRFD
                     KVYLYEPIYNIVNHFTSKDINEQLISSKPRQGSDSSSIKIKDKKEILKTIYNHGGIIS
                     LNNLQNDLDLTKYDQILTEFEEKGYIKINLKLTYPFATIILLSNDTMEHYIETENIEG
                     QDIFVKNGYSLLVNTLYISDIIRSNGLYLTQQNHFRKTDLKKLHMSVIEMQTADGLIY
                     DVEESTQLSLYLLYLLETLEITKTNVFFSIEPISKLMHMPHKMLSEIIKRISGTVGND
                     NFKAPLQVPTKKDIEFCVTTLRKKSIPANLLFAYYLHRSITPIIDTADNIDNSVDSLR
                     KSFDQTINFLVLCGIIDIKKGLLSISDTLEAPATKEPKTVFINPDFSMIIPKLDTSAY
                     AQYILLSFSSITKNDVIINAKITKESVLHAYKRGFKTETVITILEKVSANTLPQNMSF
                     LINEWIQQTLKIDINYGILLEVNHAAFLDELDFKHKDTVIRRVSDTCAIVKKETIDEI
                     IRLGEKHKAIIELENIT"
     gene            17155..18264
                     /gene="lepB"
                     /locus_tag="P5123_01525"
     CDS             17155..18264
                     /gene="lepB"
                     /locus_tag="P5123_01525"
                     /EC_number="3.4.21.89"
                     /inference="COORDINATES: protein motif:HMM:NF021981.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006465 - signal peptide processing
                     [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="HRX14974.1"
                     /translation="MYRYLKIRKNEGLYGRFSVIRGRSIPLFYIKQLLVLIFVAYSTI
                     FLAVTTPFLEKNSMTVYIIGIFILVFYTSRLLFHAIYHLYYRPRSFLTTVERIIINDY
                     HHSIMIERDDIEAIVRTFTNLLLIRTSRKTYLFPLMLIDKSQIQTLVTSFTDLFPART
                     RIIDRFFEVVHAITLALIMAVHIIQYCAQNYFIPTGSMRNTLIENDHIFAEKISYGIT
                     IPQMLGMESSMKLSWTRTRAIKRFDIVIFTPPSKSSDFHGNEYIKRIIALPGEHFSIR
                     NGSVYINGKKLYEPYTIGLTEYLGDEGLVIDGVVPSDSIVVLGDNRTDSQDSRIFGYV
                     PINNIKARAFFLYFNSDFSSGVSTRDRFSRFGFIR"
     gene            18343..20145
                     /locus_tag="P5123_01530"
     CDS             18343..20145
                     /locus_tag="P5123_01530"
                     /inference="COORDINATES: protein motif:HMM:NF013100.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein"
                     /protein_id="HRX14975.1"
                     /translation="MLLIFTGLLIVRVAVLHFSDRIVLKSDPKIENLRRGYIKDSAGN
                     ILAISIETRSLFANPEKIEDPKKTALQLAEILQIRPEYIYSRLTLPRRFIWLRRKLPE
                     ATASRIEALKINGLGFKKEFKRMYPYETLASHIIGFTDIDNNGLSGIEFSQNDYLSGT
                     ADVENADINSLNYGFNINLTIDKNIQYIAEKYLNEAVTIHNAERGAVVITDVRNARIL
                     ACAVNPAYNSNYYFRYTGKQRASYSVVQSFEPGSTMKIFSAAAWLESGRADMNKLYHG
                     SGEIDIYDVTIRSTSVHGWITMFDAVRLSCNVSVIRAMQEIRKDELDAMLRKFGFGSK
                     VCRDFPGESPGLLRPVNEWSGISKASISIGQEMSATSLQLAAAFGAVANGGVYIQPSL
                     IDSIELNNGHKIQQFYPKSKGRIISRQNGEILRQMMKAVVETGTGKNAAVPGYEVAGK
                     TGTAQISSSTGGYLEGEYTASFGGFAPYDDPLIAVVVVLDRPKGQVHGGEVAAPVFSK
                     VVSEVLPLMGAGAALVTNIDPREISARSPHFDGEHMPDLYGYRLSELTPLFEHIQKKY
                     AVQFEFSGSGVVTSQSPEAGTVLQNKQKIELHLR"
     gene            20148..22007
                     /locus_tag="P5123_01535"
     CDS             20148..22007
                     /locus_tag="P5123_01535"
                     /inference="COORDINATES: protein motif:HMM:NF014075.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF115 domain-containing protein"
                     /protein_id="HRX14976.1"
                     /translation="MSYFEKNITILQKKDPALVVSLKKAKTEFISVCDTKTGDKVPLV
                     TLNEGKRPVAVHSKFDPDREAARLIDGLDLNTHNLFVVIGFGFGYHVQNLLERINGDS
                     LVLAVEKELGMLKAALTERDLTKSLSDERLLLLVDPDEDIIAQTLKGRSSRSVVFVTH
                     RGSFQIDQDYYSNVNRLMRSYLSSKEVNIATLAKFEKLWTLNCAKNALQHISLPGAKI
                     FYEKFEGLNAIIIGAGPSLRYSLEALRQNQNSVVMVALDTSYKILIENGITPHFCLCV
                     DPQLINARYFEGVPERDTVLVADPTAHPSVFRLFKGPQVVCSMPFDTMKWLEEYTGEK
                     GEVAHGGSVSTNAYDFAKRLGVNNIYLVGQDLSFTERLAHCRGSYLDEMMFVRIDRFF
                     NQEMINRRQLTASPKIMVPSIDGEKVHTNQKMVIFQKWFEKRNDAHLFNSSVNGIKLN
                     GIKDIELNAAFTACENNVSEMVKSIYLEASIPHETKIEIVKKVSGRVVLLRNENNVVM
                     ERLENAVNTAEQLIEALKKRSKQVGALVTRLDTIDEFIASKSSAKSMIGMSIQRTIHT
                     ITEGYDTDEFSGSGHEKIARKSLFLYKSLLEGCLFAEKVLNKSEKIAKTFICN"
     gene            complement(22033..22809)
                     /locus_tag="P5123_01540"
     CDS             complement(22033..22809)
                     /locus_tag="P5123_01540"
                     /inference="COORDINATES: protein motif:HMM:NF024929.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M15 family metallopeptidase"
                     /protein_id="HRX14977.1"
                     /translation="MNNFISKYIIFNTLFVCFALISSCAQYQIGSEKFFSQFSQRVIV
                     DCDMTLDEALANVPEECPESVVDNLALIEVFYYSFDNRIHRGQILADYRHAEDIHAIF
                     TVILRERFPIYGCIPIDHSSYEWVQFNTVPEGNSYSFHYRNIVYKNTLSYHSFGHAID
                     INPIINPFRKWGTTFPKGGTYDPQKPGTLYEEHPVVLAFKKHGWYWGGNWRRRDYQHF
                     QKPIKGENDIHIVSNPGIIRWPNKQFSESDGSERYYTTNP"
     gene            22976..23203
                     /gene="rpsU"
                     /locus_tag="P5123_01545"
     CDS             22976..23203
                     /gene="rpsU"
                     /locus_tag="P5123_01545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250651.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S21"
                     /protein_id="HRX14978.1"
                     /translation="MASPLIVNVDNGDLEKAFKNLKKKMALEGIFKELKRRRYFEKPS
                     QEKKRKREEAERRRLKKIRRLAAQNRRSSRK"
     gene            23265..24554
                     /locus_tag="P5123_01550"
     CDS             23265..24554
                     /locus_tag="P5123_01550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008671826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="replication-associated recombination protein A"
                     /protein_id="HRX14979.1"
                     /translation="MTLFNDNSSLVPLAERCRPHTLKEYIGQLHLVDNDKILFRMIEE
                     KRLFSMILWGPPGCGKTTLARIISDTLNLDPHYLSAVSAGVADVRKIIEKGRTNRLHG
                     VASLLFLDEIHRFNKAQQDSILQAVESGDIVLIGATTENPSFSIIPPLLSRARVFKLE
                     PLKTGELRSILDNAIKNDSLLKIYDIADNLRDEIVKGGGGDARKMFNLLESACALSLG
                     QTLTADAVEQAVAASSYYYDRSGERHYDTISAFIKSIRGSDPDATIYYLARMLNGGED
                     PLYIARRMVVHAAEDIGNASPTALTIAVSTMTAVKNIGMPEARIVLSQCALFLASSPK
                     SNASCMAIDEALDFVKSDVSQIPMHIRNAPTKLMEEFGYHKGYKYPHDYPGNFVQENY
                     FPEELKDKVFYNPSENGSEKSIKERLAQLWPRRNRKG"
     gene            complement(24555..25097)
                     /locus_tag="P5123_01555"
     CDS             complement(24555..25097)
                     /locus_tag="P5123_01555"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRX14980.1"
                     /translation="MTTKLLNIFMLLLITGCALIQRAPFGKKAVYNATSSSISVSLNE
                     IDSHPTYELFFMLNEKESEIKASINGQIINAAGSSVEGRFFFIIEKKYDTSSSDSIYT
                     SYIELGRNFDNSWNSSESVQISSGSDGLTSLDPGIYRIRITIFSDNIFDCKISLFTSA
                     KEAHFSDKAVFDSDVSENKD"
     gene            25338..28085
                     /locus_tag="P5123_01560"
     CDS             25338..28085
                     /locus_tag="P5123_01560"
                     /inference="COORDINATES: protein motif:HMM:NF014516.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HRX14981.1"
                     /translation="MYLKSMEIFGFKSFADRTPVTFEPGITVVVGPNGCGKSNIVDSA
                     KWVLGEKQAKNIRGTRMEDIIFTGTEHRKPLSLAEVSLTLDNSTRLLNFDSESVTVTR
                     RIFRDGESEYLINKSPVRLKDIEQLFMDTGIGKTSYSVMEQGRIDMILSSRAEDRRYL
                     FEEAAGISRYKVQKRDSLKKLSDTSENLDRINDIIHEIDREKQNKAKQAEKTKEYIKF
                     KSELKEFDLKLQALKYRDLHKRLSKIVENRDKLVEESKAISQKISGISEENERDEKRK
                     NEIQLQLFELDKKMHTYRIKVEDIDDKREKNNKLIQENREQRERLLAEVEERRISYKN
                     LTEEKARNQKNGSEIEQKIAEDRNQLKIFFERRKQKIEQIHNNRETIDKNRLFVTDSE
                     KTLKSLRDELEVVIKQLIDAIDKRKAELLDSETERQSVRASIHEQVADIREKLSAAIN
                     AIEEGGDCCNLLSSIDIDKLNESISRFESFEDGFRSILFDKTGIHARKESIDKKIADC
                     ALRIEEARAQNISLEEQNVTLQGELEDVNEMITRIEKDLSRNDNERVWIEKHVQGLDR
                     QIEDIEKHIKNVHTEIKRFEEKTEQLLAEIKDWEEKLVEFSERSVSLKKRIEESSHKR
                     EEIDKKIVSRKQSSARDAEKLNEVNTKISDLEKKSVEIEFKKNSAEEYIWTEYELKPE
                     SIKKVKVDELELEKINEQIGKLKKSIERLGPINNLAIEEYNDLKKRFDYYSEQRDDIM
                     KAREDIISVIEDINQTSVRMFVETFEQIQKNFTEIFKQLFEGGHAEVILADPENVLES
                     GIDITVRPPGKKPKTINLLSGGERSMTAIALLFSTYMVKPSPFCFLDEIDAALDEENV
                     RRFLKMMRQFSQKTQFIIVTHNKKTMTIGSSIYGVTMEEPGVSKLVSMRLGSDNSSNL
                     A"
     gene            28370..28918
                     /locus_tag="P5123_01565"
     CDS             28370..28918
                     /locus_tag="P5123_01565"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRX14982.1"
                     /translation="MNITADGILGSARKINQKRTSLSETSGPKSTAGKSDSVQIETRL
                     NGRLDTLQADLKATQTSLTRNQIIHAGLKDLAEDFRKGGGRSAEIISQVTYNGEQPLS
                     ELIGDNTLNEKFLDELMKGTVIRINKDIADLSRMQVESENIFASSLISDPSSRMTDMF
                     QNNVSGESISSLNADVVMRLTR"
     gene            28964..29902
                     /locus_tag="P5123_01570"
     CDS             28964..29902
                     /locus_tag="P5123_01570"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRX14983.1"
                     /translation="MVRNIFFTVIVLLSFSLYAAHSISGSDYLLFQLGDPSVLPGSGW
                     VNHADATQLLEWGRAKGLNLVEDMAPVANRRASFIAASSASLQVTGLKAHATYTLWID
                     FVKFRSEKNPGIYSKLHVYADGRQIDELVWGKLPPDTLYRIDLPLDLTYDGDVVITFK
                     EYAMNYGYWGVWDMVLATGDLPDGSYFKGAAPSVSPEDSSVSDKGKVSDLKESSDDSR
                     IVVDKTKKDIEKPATVTKKTPVKPKPAVRKPSSKTKPKSPSKKPTVSKPKPKDVLPKK
                     PSVKTPSVTSPVEPIVPFVDEPIVDRPVIPDKPKSR"
     gene            complement(29961..>30454)
                     /locus_tag="P5123_01575"
     CDS             complement(29961..>30454)
                     /locus_tag="P5123_01575"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRX14984.1"
                     /translation="EITDFRTSIDVEVSRLEIIEEYAAKHDMDISDVMNTVLDMYSKK
                     LAKNPVCNDTLNKYHERGTIYKSVHIRFSSITKRKLKDIANLYCYSLSYMLFLAMDFF
                     DEFMVGVKEFIRPCFHASFVYHFFGFPYFINSWGMNFYEGQGQPTGQESKKILNLPYL
                     NKL"
BASE COUNT         9667 a         5035 c         6314 g         9438 t
ORIGIN      
        1 cctcaagctt gattagatct ttagtcgctt cttttatgat atagtagttc ttctttataa
       61 tatcggcatt attgtctttt ttatagcggt atatgtatag aagattatta ataatagtgt
      121 attggtattc acttttagct ttaacggatt tatttatcac tgaatagatt gtgacactgc
      181 tctttgtgaa ttcataaata acagaaatcg aatttgtatc gtcactatcc ctgatttccc
      241 aacgtcctat aatatcgtaa ggttcagaaa gtaggtgcgt ccataggttt aaaaaaataa
      301 gggctataat ccatttagta ttcactggtt tttttctagg tcttatttgg taactcatta
      361 tcccgaaact gcctctttaa gaaagcgttt ttaaatctat aaaatgtttg aaggttttcg
      421 acaatctatc gacaacccat ttataaaata tatgtgattg tgcagtcgat acactacaca
      481 taaatatttt atccccaaac gatattaaac tgtcaagaat aatcatttgg caaaaatgtg
      541 atttatgaga catattttgc taattgtatt ccagaaatgt gccgaagtgt catataagga
      601 tgcctgtttg ttattggtgt ttgaaacagt actccgggag gtggatatgg agttagcaga
      661 taaactggtt attgttcttt cggaacaggc tgctttatat agagatatgg aatcgattga
      721 acaacaaaaa aaagagataa taatcgcacg tgacgggaaa gaacttgaaa gactggtttc
      781 cagacaggag aaaattatca ctgacattga catacttgaa aaaagacgga ttaaaataat
      841 ggatgaaatt tcgttttcaa gtggtagaag agaacctgta attacggcaa gacaggctgc
      901 cgtttgttca gatagacgtt caggtgaact gataatacgc tacgcaaatg aattaaggaa
      961 tatagtaaaa aggataaaaa aattatctga tgttaatgaa agatgtatga aagataatat
     1021 ggagtttttc acattgataa ttgattcgtt gcgacagact gttcgaggtg aagaagggta
     1081 ttcggcttca ggggttgcgc ccaagcgttc ggcagagtcc gcgcttttca accttactgt
     1141 atagtggagg aataagatga gttcaacatt tatggggctg gagatcggaa agcgtggttt
     1201 atccagtcat caacaggcac ttcaagtaac tggtcataat atatcaaacg cagaaaacaa
     1261 ggagtattcg agacagcgtg ttgtcattac ttctgctgat cctatttatg agccggcttt
     1321 taaccgagca gaaagggctg ggcaaattgg tcaaggttct acagtagcac gcatagagag
     1381 aataagagac gcctttgttg atgatcgcat tgtgaccgaa aaaaattctt taggttattg
     1441 gcaaaccaaa gacaaatatg tttatcagat agaaaacata tataacgagc cttcggacaa
     1501 ttcaattcgt actaaatctg acgagttttg gaaatcatgg caagagctat ctaaataccc
     1561 tgaacagcga tcaacacgag aagtagttaa agagaaaggg gtagcgcttt ccaatgagat
     1621 taatcatgtt agccgtcaac ttcttgattt gcaacgtgat gcaaaccgcg aagtagcatt
     1681 aagggtagaa cagataaatg aatacggtac aacaattcgt gaccttaata tgcgtatatt
     1741 gaaggctgaa tcgcttggcg atagtcctaa tgaccttttg gatagacgtg acgcgttaat
     1801 cgaaaaatta tctcaactgg tcgatgtctc tgtaggtcga agtgatgaag atgaattgat
     1861 tgtttacatc ggatctgaaa actttgttca gggagaggta ttgcataaga ttgctgtaaa
     1921 ggaagatcct gataatcaga gtttttacaa ggttgtttgg caaaaaacag aagctgatgt
     1981 gactttaaaa agcggcgagc tacaggcttt gcttgatgta agggataagg tgcttcagca
     2041 gaatattgat gatatagatt ccatggctat gaatatgatg gatctgacaa atgaagtgca
     2101 ccgtgacggt ttcggtaaaa acggaagaac caatatcgac ttttttcgca ccataagcct
     2161 ctctgaaaat attgagggaa acgctgattt aaataatgac ggtgaagatg atgtaagtgc
     2221 catatttaag gtagcaggta aaaacagaat cgatgcgtca gcagctatcg gaattaacgg
     2281 aacgctacta tttcgtaaaa atgatggcac agaaacgctt cttcctgtcg attatgtttc
     2341 aacagataca gctaattcag taatacagaa aattaataat gcaaaacttg gtgttgtagc
     2401 ctatatgaca catgacggta atttcgcgtt aaaggcgacg gctgctctag ataatccatc
     2461 cagaaatttc atgattcgtc acctagaaga ttccggccag tttttggttg gtttaaccgg
     2521 agtacttcaa aattctggtc cggatggagc ttatgaatat acagagctta atgcagtacg
     2581 taatcttgct ccagagggtg atcatataac actaacccca agaaaaaatc catccgtata
     2641 tatggcagtc agtaaagaaa ttattgatga tgtggataat attgcgtctg cgcaaggaat
     2701 agattctgac ggcagcggag actataacct ttcaaatggt gtaggagacg gtcaaaacgc
     2761 tatggctata gcttctattc gtcacaagga cgcaatggtt cagacaagct ctacttttga
     2821 tgatttttat atttcgttaa tttctcgtat cggtagcgaa ggcgaagagt ctcgtgatcg
     2881 tgtaaataat caggagatac tcctttcgaa tctgggaaat ctgcgcgaat caatatcggg
     2941 tataaatctt gatgaagaga tggccaatat ggttgctttt caacatgggt ataacgcttc
     3001 agcgcgtatg atttcgacat ttgacaaaat gcttgaaact ataatcggtc ttggaggcag
     3061 ataatgtacc gcgtaacgaa taagatgatc acaaatacaa tgacgcataa tcttcaacgg
     3121 cataaacgtg aaatggatca ggcacagaat tcgcttgcta ccggaaaaaa agttcgaact
     3181 cctagggata atccaatagc aacaacgaat caaatgcagt atcagacacg gttaaacgag
     3241 gtaaaccagt atattgagaa cataagcgaa agtaagtctt ttcttgaaca gactgactct
     3301 tcgttgcagt cgatggtacg aatttttcaa agaatacgag tcctcgctgt tcagggggct
     3361 catggaatat attcatcatt tgagcgaaaa gaggctgccg ccacagagat caatcagttg
     3421 ttagaagaat tggtagctat ttccaattcc aaagacgcga ctggaagatc tcttttcggt
     3481 ggatatgaaa cagggtcaac agagaacccg aatccttttg taccgatata catgactctt
     3541 actgctggta atcaaggtga cgcgatgaca ggagttgagt acagaggaga tatgggaaga
     3601 ttgacgcgtg aagttgctaa gggagaatat atggatatca gtgctcccgg aagttatgcg
     3661 atgtgggcaa caaatcaggt cgttacatct aactttgacg cctcagagta ttcatcaaat
     3721 gccaatcaaa aaatacgtat cgacggggta gaactgaata ttgcgcctgg agacaatatc
     3781 gatataatta tcgataaaat taataatgcc ggtctttctg taagggcatc taaaggcgga
     3841 cgaaataatc ttattatgga aacaacagca cctcataaga tgtggttaga agatgtagga
     3901 gacggtcaga tcttaaaaga tatcggtatg ataaaccctc taaaagatca gccgcctaac
     3961 aattatgctc caaccgccac agttgacgga cactcaatat tcgatatggt tatacagctc
     4021 agggatgatc tagtacgtgg agaccaggag ctggtgggtg gaagagacct gggattaatt
     4081 gatatggggc tcgagaatat tctaaaacat cttgcagaga caggagccaa gcaaaataga
     4141 attgaacagt tagctacacg ttcggaatat atgaagacaa atgtaacgga aattcttgct
     4201 aaaaccgaag gtattgatta tactgaagag ataatgaatt ttaaatggat cgagagcgtt
     4261 catcagtatg ccttgtctgt tggtgcgaaa accataaaac caacattgat ggattttctg
     4321 aggtaataaa gtttattaaa atagctctgt gatgcttgaa aaacgaggtg cttctttgac
     4381 tatgacgatt aatactaaac aatttggatc catcgatatc gatgaatccg aaatcatttc
     4441 ttttcctgac ggtctttttg gttttgaatc attaaaaaaa tttacattaa tcgaagaacc
     4501 tgattctccc atgatgtggc tgcaatcctg ttctgaaagt gatttggcgt ttgtaatttt
     4561 acgggttgtc gattttatga aagagtatga cttgtcaatt tcgcagtctg atctggaatc
     4621 ggttggtaca gaagatcctt cagagctgga tgtttttgca atagtgacta tccctcttga
     4681 gaacccttca gaaatgaccg ccaatttgat ggggcctgta ataataaaca tgaaagataa
     4741 aaaaggacgc caggtgatat ctttatgtga taagtataaa acgaagcatc gcgtactgga
     4801 cgagcttcaa gcgaaaacgg gggcctaaaa tatgcttgta ctggcgcgaa gaacaaatga
     4861 aagtataatg attggcgatg atatcgaaat agttgtaata gatataaagg gagatcaggt
     4921 aaaacttggt ataaaagctc caaaaaaggt aactgtacac cgaaaagaga tatttgacga
     4981 gataaagcag gagaacatcg cggcaatttc tacgccgttt aaacctgata ctctacgaga
     5041 tttgtcagat ttttttaaaa aaaagtaaaa ctaggctata acaaagtgtc atcagagaaa
     5101 gatgtctaat taagttattt atgtagaaat taaaaaagag agccgcgatc acgcggctct
     5161 cttttttact tgcaatatga tcgctatcag ttattatgag acataattat taatttcttt
     5221 gttgataagg cttaagtaag gctgttcaag atatgtgtag tatgaggtta ttatgaaaaa
     5281 aattctgcta tttgcagttg ttttatcggc tactttcttg gtcgctcagc ccaaaaatgc
     5341 ccgatactat aataaggaag ggtggaatta tcttgagaaa agggaccctg tgaaggcaat
     5401 ggcgagcttt aaaagtgctg tatctcagaa caaggactac aaagaggctt tgacagggct
     5461 cggtttttct tacctaatgc gtggtgctgc ggatgatgct tttgaaatat tcaataattt
     5521 gtcaaaaaaa tatccggagg acgagaagat tcttcttgga cgagcagctt cgttaggaga
     5581 gatggggcgt tatgaagagg ccttaaatga ttatgcgcgg gtcactgaaa tcaggggcga
     5641 gaacagcgaa gctctttacg gaactgcctt aatatactat cgaatgggta aaaatgttca
     5701 agcaaagaga accattgatg aatcacttcg agtaaatcct tatcattatg atacattgat
     5761 acttgatgcg agaatacatc ttgatgagca gcgtcttgat caggcggaag gtataattca
     5821 aaaagctatt aatgttaggc aggaattccc tgatgcttat gtgcaatatg gaaaattact
     5881 tttaaatcga tataaaaaag agtcaaatcc tgcattttta tcagattcgc gtgaggaatt
     5941 taaccgtgca atttcgataa acccctttga tccagagtca attagaatgg caggaataat
     6001 agatcttatc aatgatgatg cctcttcggc acgcgattat tttagcaagg ctcttgatat
     6061 aaatggcaat gatttcgcaa ctctgtataa tttgggatac agttatgaac ttttagcaaa
     6121 tactgataag gccgctgact tttaccaacg agcaacaaat cttgcaccag atgatgtatt
     6181 tatgaacagc aagatgcaaa cattcttgat tgaaaataat tttgcatttg gtaatcctca
     6241 gagggttcaa tacgctcggt taaattacag tgaatataaa aaaaacagat cgcgaaatat
     6301 gtctgattat gcgttaatgc atttacgcta ttgtgttgtt ttaaatccaa tggatcagga
     6361 tgctcgtaaa gagttggctg cattttataa tattgcgggt tttaacaggc tttatatcaa
     6421 tgagttaaag gttatacaac gtcttcaccc tgataacagt aacagggagg ctcttaacct
     6481 tgctgttata cgtcgaagag acagactcta taacaggtca gggtacacac tcgatatgcc
     6541 tccacgtaat gtcgttaagg ttctggtttt accgttttat gaccgttcag gtaattttag
     6601 catgtttgat aaaggtgatg tgttttcgga taatcttgtt tttgctctat ctcaatttgg
     6661 gcgaattcag attttagacg cagccgagcg aaaaataatt attcagaaga tgaataatta
     6721 tgataatatt tccgaagcac ttgcgaaaat ttcagacgaa gatatagatt ttattgttca
     6781 cggttctttt aatgctggga agacatatat agattccgag tataatattg tcagtaatca
     6841 taccggtgct gttcataaaa cttttgagct tcatgaatca ggtgaatcgg ctttaaacag
     6901 gataaatttt cgtgctgcac gcgatattgc cgatgcggtt ccgttacgtg gaaaaatatt
     6961 aaaagttcaa aacggtgaac cattggtcaa cctggggctt tacgacggaa ttgaaaaagg
     7021 agctaatctt tttgtgtaca gctcttctgg aaacactgca aagggaaggc gcataatgct
     7081 aaaagtcaag gagaccgatg cctttttatc cacggctgcc gtttcgaaga gcagtgaggc
     7141 cgatatgctt gttacaggta tggatgttta tgaacttaaa ataagacgtg caaggcttat
     7201 aaagtagatc aattgacaat tttttagttt acagtgtaga atcgtttatc taatttatta
     7261 gacttgaacc aggtttatta cgaatcttgt atattatggt ctgtttttaa atttagttag
     7321 ctgatattaa ttatttggtg tcttcaaact gttctagagt caatcttagt actttttttg
     7381 aagtttcttc atactctttg ttcattccgt taagtttgta tagctcttga gccaattcga
     7441 gatattgaag attgtcgggg tcaaattcaa gagctctttc tatgttaaaa agtgctgcct
     7501 ggtaatcatt gtttcttctg tgcagtactc tctcagaata atagatattg gcaatcatta
     7561 catagatttt tgtttcatga ggtaaatagt atttaagtct ttcgagggtt cttatcgcac
     7621 tttcaaactc tttttctgcc cgataaacac ttgaaagctc aaaatatatt tctttgtgcg
     7681 caggattaag tgtcagagct ttttcaagca gtgcaagtga cgacttatta gcttttttgg
     7741 ttcttttgtc tctggcctga ctgaaaaggt tcatgctttt ttcacggtta atttcgaatg
     7801 ctctgttaaa gtgataatca gattgttttg tgttcccaag aatgctttcg gttcgggcac
     7861 accctaaatg agatttataa tagttatcgt taatttctat ggatttttta aaatggctca
     7921 gtgcctctgc tgttttcatt ttgcggtcgt atatttctcc aaccatatag tgggcatcat
     7981 cctgttttgg gttggtatcc aaagatagca aaaaaaaggt tagcgctcgg ttgtaatcct
     8041 ccttataatg ataaagaaaa cccaaatact tggcgctgcg gtaggcttta ggattcatct
     8101 ccagagcccg gttcagatgt tcatcagcga aatcgtacat ctgttcttca tattgaatta
     8161 ttcccttttg ttcaaatatt tcccattttt caaatttttg agcaaacatc tgtgatgata
     8221 aaataaataa tattaacgaa aaaaaggcaa tattcttgac ataaactatt gataaaatat
     8281 aaatgtcaca gtttcgaacg aggtcaggta gctcagtcgg tagagcaggg gactgaaaat
     8341 ccccgtgtcg gcggttcgat tccgtccctg accaaaagcc ggattttatc cggctttttt
     8401 tattgtgaaa ttttatcttc tttattgaac tgctcataaa tacccgctct gtctgttttt
     8461 tttatttggc ggttcgcccg gataaaatag aacttaaaac atttatggtt taccggtatg
     8521 tactctttct tatagtatcg gcaacaattg atctgttttg tattacagaa atgaacttcg
     8581 aaaacactat ttccgtttgg atttgtttat tgtaaaatat ctgtttatag ggggaaaagg
     8641 cgatgcttga tcctaaattt gtaagagaaa actatgatta catcgaaaaa atgcttaagt
     8701 tgcgtaaaat ggaaggcgtt tgtgatatta atgaacttaa atcattggat gacaaaagaa
     8761 gggatctctc atctgagatg aattctcttc gcgaagagag gaatagtgta tccaagtcta
     8821 ttggcatacg aaaacagaaa ggtgaagcct gtgatgacct aatggagcat gtacagacca
     8881 ttaatgataa aattgaagca ataacagact ctctgaataa atatgatact gcttataacg
     8941 atataatgct ctcaatacct aaccgtttgc atgagagtgt cccagagggt accgatgaaa
     9001 gttttaacaa ggtagttcgg gaatggggag aaaagccagc ttttgatttt gaacccaagc
     9061 cacactatga tcttggagcc gatttgaaaa tattggattt tgagcgtggc gtaaaacttg
     9121 ccggcgcacg tttctacgtt tatagaggac tagctgctcg gcttgagcgt gcacttattt
     9181 cgctaatgct tgatactcat acaaacgaac atggatatac agaagtcttt gccccatttc
     9241 tagtaaacga tgagtccatg atcggtacag gtcaatatcc aaaattcaag gatgaatatt
     9301 acaggcttga tcgtgataat ctgtcgctaa tccctacggc tgaagttaca ctgacaaatc
     9361 tttaccggga tgaaatatta actaaagagg atctcccatt gtatgttgcg ggtcaatctt
     9421 cttgctttag gcgtgaagcc ggatcggctg ggcgagacac acgcggtctt atacgtgtgc
     9481 atcagtttca gaaagttgag atggtaaagt ttgtcgatcc agagagctca tttgatgagc
     9541 ttgaaaaact ggtagctaat gcggaagcaa ttttacaaaa actgggtttg cattaccgag
     9601 tattgcttct atgttcagga gatacaagtg cttcgtcatg taaaacttat gaccttgaag
     9661 tctggatgcc gggattgaac cgttacgtag agatttcttc atgttcaaat tttcttgatt
     9721 atcaggctag aagagccatg atccgatgca aaagtaagga tggcggtaag ccggaatatc
     9781 ttcatacttt aaatggatcc ggagtagcgg ccggaagagc tctagccgca gtaatggaaa
     9841 attatcagca caaggatggt tcaataggcg tgcctgaagc tttgaagaaa tacctagaaa
     9901 gatagttttt agatgattat cagtttatat cagactcttt tataaagtta aaatatttgt
     9961 taacaccagt tattttaaag aggttatcta tatctgagtt tatgttcatt ataaaaagat
    10021 ccattttctt gttttttagc gtttgcagta ttcttacaaa tgctccaaga gcggaactgt
    10081 ttagataggg aacctcttta agatttataa tagttttatt tttatcaacc gatatatttg
    10141 tgtatatata atcttccatc tcttgagttt cttcaaaggc tatattgccc ttgatgtcaa
    10201 agatctgata ctgattattt gttctgttag aaatttccat ggccaccctg ttaaagtttg
    10261 atagtataat aaaattcaag cacggttaat aaggacaggc ttctgtatca aagtaacaaa
    10321 aaaaaataat taatcaaaaa aattgataaa aatctacacc aaagttataa ttttttcttt
    10381 tttagcgcat tgtatacata gtctgtaccc tttttttgag cgttgcggta tataatagtg
    10441 atttagtctg tttatttcga ctccacatct gctacatttc atggtctcct ccattttctg
    10501 atctaatatt attgtcggat gataattcat tattataaag tgtgtaatat taacggtaat
    10561 tatttagggt atttagagag agcttaacca gcaattaaaa cttgaaaatg aaccttttgt
    10621 atgtattata gtcatattta ttattgggca attttaacgg actactgttg ataatttgct
    10681 gattttctta ttttatctgt ctctgttata ttttgttgta tatttatatt tcggtcgttt
    10741 tgacgataag aataaaaaaa atgtagttta caatagggag tcttttttta gtatcggtta
    10801 ctaatagttt tcaggagtag tgctaatgtc atctaaagct gttgttacaa aatcaaaccg
    10861 taaaggccga gcaaaacgta aagttaatct tatacggctt cacactgcga tgaataaact
    10921 tgcgtctgac gcaaatgaca aagagattgt taaaagattt aaaatactta ttgatacatc
    10981 tgatgaggat cttagcaagg ctagggtgga tgaaattcta aaaacaccgc aaaaagttga
    11041 aataaactct tttcatgaaa gtctgcaacc gtatgtgaag cattttctgt ttctagctaa
    11101 gaaaaacagc aagtaaggat tcaaatgagc tcaaaatatc ttgtaatagt cggtgacggc
    11161 atggcggatc ttccgcatga gcaattaaac ggtcaaaccg ttatagaggc atcttttatg
    11221 cctaattctg ataaaattgc ttcttttgtc tgtggcctga cttcgactgt acctgatggt
    11281 atgaatcccg gaagtgatac tgctaatctt tccattttca gttataaccc tgctcaatat
    11341 tatacaggac gagcccccct tgaggcctta aatcttggta ttgatcttgg gccaaacgat
    11401 atggctttcc gtatgaattt tgtctctatc gttcagaatc gtatggatga ttttactgct
    11461 gatcatatcg attcccaata tggtaaattg atagttgatc aactcaaaaa gttaaatcat
    11521 ccagatattg aattttatgc aggcgtttct tatcgtaata taatggtttg gcgaaattat
    11581 ccttatgatg aaatgcctct tactgttccg cctcacgata ttcagactat ggatgtttca
    11641 tcttatcttg caagtggttc aggagccgat ctattgcgcg aaatagcgtg tgaggctcgt
    11701 ctggttatag ccgatgttgt atctgaagat cacgggcttg gtggtaatcc tacggatata
    11761 tggctctggg gagctggtaa gaaaccagca ttaaaaagat tttcagaaaa atattccata
    11821 agtggagtta ccgtttccgc agttgatctg atacatggga tagggcgtgc tgctggtttg
    11881 aatccggtaa ctgttgatgg cgcaacaggt tatcttgata caaactataa aggtaaagtt
    11941 gatgcagcgc ttaatgcgtt tgaaacacat gattttgtat atctgcatat cgaagctcct
    12001 gatgagacgg gtcatgaggg gagtcttgag aaaaaaagac aggccgtaga agactttgat
    12061 ttgaaggtag tcggaccaat gcttgaggga atgaaaaaat ttaatgatta tacaattctt
    12121 ctaatgcctg atcatcccac tccagtgtct gtaagaactc atacttcgga tcctgttcca
    12181 ttcgcattta ttagtaccga accacagttt gtggatgtcc atagaaagta ttcagcttct
    12241 agttattctg aaaaggaagc taaaaacacc ggattattcg tgaaagatgc atctatgctg
    12301 cttgatataa tcattaagaa aaaaatttcc agtataatgt gaagtgcggc atgagttcga
    12361 aacgagccaa tttgttcata gtagtattat taatattgct tattgaatgc actggtgtcg
    12421 cgccggaaca tagagatgaa aaatctaatg agaatgttgt tccttcagat gactatataa
    12481 tagacgagat tgaggagttt acatttgatg atgtagaaga gcaaagcgga gatccaagac
    12541 ctgaggttac ttcgttacct ggagcagcat ttagaattca tctgagctcc ttttcgtaca
    12601 gagttgaaca gctaaggtat tttaaccggc tttctcttgt gcgaaacgac aatcgggagg
    12661 gtggtttaag aagtattgct gcgcgttata acagaaccga ttcagtaaga agcgctcacg
    12721 tactaatctc tatatttcct tctaccggga ccttgaagca gtacaggttg ataaagtcaa
    12781 catacatcaa ggaacttgat gccttgatta tgaaagatct gtcaactatg cgtttttcaa
    12841 ttgtaagaaa taaaattcct tctgttctat atatagcata tactattcaa ttatcagatc
    12901 aaagacgtta aaactgtaat tgatgtttta gccattttgt ttttatataa tcatttatca
    12961 cattcaatct gtttagtata tgttttatta actttttatc gctttatcaa tctccatgcg
    13021 cattttatta attgtagcac tgcagtcttt ggagtgaaaa accgccgatc ccgaaacaaa
    13081 ggtgtctgca ccggctttag ccaaggatcc gatgttctct tccgtgatac ctccatcaac
    13141 ttcaagtctt atatccctgc cggatgagtc tataatctct cttattgctt taatttttgc
    13201 aaatgtcgat gggattaatt tctgaccgcc aaaacctggg ttaacagaca taacaagaat
    13261 catgtctact ttatcaatag tatatgttaa ccattcaaca ggagtcgctg gattaagaac
    13321 tatcccggct tgacagccgc aactgtgtat tagctccaga gaccggtcaa catgatgtgt
    13381 ggcttcaggg tgaatgctaa tataggttga ccctgcttta gcaaagttta taataagatc
    13441 atcaacagga gttgtcatta gatgcgtatc gataggggca gtgatcccat attcacggag
    13501 agccttgcaa accattgaac caaatgtcaa atttggtaca taatggtggt ccataacatc
    13561 aaaatgtatc atatccgcgc ctgcttttat tacgcggtca gtatcttcgc ctaatcttgc
    13621 gaaatctgca gctaaaatag atggagcaat taaaaaaggg ctcatttgta cctcttaagc
    13681 ttctcttggc ataagcttct tcactgtatt agaatatgcc caaactaaaa aagggttgag
    13741 tttttttcaa tcttttaaaa tgattgaatt gagttaacaa ttattattta tttataaggt
    13801 gggctctaga tacaaaaata atattcgatt ttaacattat ttaagaaatt aacaaaaaaa
    13861 tggtctaaaa aggactaaac tggagaaatc atgaaaagcg tagatatcaa ttactttacg
    13921 gataaccagg caggtgaagc agcaaccatt caactgaaaa aactggggct taacgttaat
    13981 cgggctgata tcgataaaat ttcttccata agtcccgatc ccgataaggt aactcttatt
    14041 gttgccgaca ttgattcaat gccggtaagc gatattatag aaaagtttaa tagtttacct
    14101 aaactccata acattgttaa atacatcgtt ctcgattcat cacttattgt gcatgccggc
    14161 gattaccgag gttctgcaat gcatacagaa tttattgaaa agccttataa ccgttatgaa
    14221 tttgcattat taattgaaaa aagtattata gtcgagaaat atcgtcaaat catgaaacag
    14281 ctttctaatg agtatgaaaa aagaattgaa cagtacgaga atatattcca cataagcaat
    14341 agtggtgtaa tagctactga tcaagataaa aaagcctttg aaaaaatact ggattttgag
    14401 aaaaatctaa taaagaagca agaggaatta aacgaaaagc ttcatgattt ttcatctttc
    14461 aggcagaaag atcttcttga aacacaaaac ctgcttttcg caaacactat gcttgaccgt
    14521 cttagagaga aggagcttat ggatgctaat gagctgattc acgcacagga gagggttctt
    14581 gaatattcta gtagcagact tggagataat cttaaacttg ttgaagctca ggagaaggtt
    14641 gtagaattaa gtcgtgatga ggctatttct cttcatcata caatagaaaa tttgataaaa
    14701 gagaaaaagt accttattga agagaatcag aaattaaaag atctgttaca aaaaaaatga
    14761 actagccgtt atttattaat gagttggcgt ccatatatgg attttataaa aataaaaata
    14821 tttcttattt caaaatgatg ctagctttgt aatgatatgt ttaaaaaatt gtgtttaatc
    14881 gtaaaaggaa caaatagatg gaatgggaga aacttctaag gaattctgta aaagacggca
    14941 ctataaggga gttatatttg cgtaagattc cggtactaaa aaactgtgat aattggaata
    15001 aaattgagcc tataggttat atcgatactc cgatgaagta tacccattat aaaggaattt
    15061 tggtaatgta ccaggatcgg atcttttttg ttacttcgaa aaccatggaa gctctaagtg
    15121 agttcgtgaa gtggaatatt cgcaataaaa tcagggttat tcctgaaaaa aaatagatca
    15181 ggtgatgttt tcaagctcga taatagcctt gtgcttttca ccaaggcgaa taatttcgtc
    15241 tatggtctcc tttttaacta tggcacaagt gtctgatacg cgcctaatta ctgtatcctt
    15301 atgtttgaaa tctaattcgt caagaaacgc ggcgtggtta acttcaagca atataccata
    15361 attgatatct atcttaagag tctgctgaat ccactcattt attaaaaacg acatattttg
    15421 tggaagtgtg tttgctgata ctttttcaag tatggtgatc actgtttctg ttttaaagcc
    15481 tcgtttgtaa gcatgtaaaa cagattcttt ggttatttta gcgttaataa taacatcatt
    15541 ttttgttatt gaactaaagg agaggagtat gtactgggca taagccgatg tatcaagttt
    15601 gggaataatc atcgaaaaat cgggattaat aaaaacagtc tttggttcct tcgtggccgg
    15661 agcttccagc gtgtctgaaa tggacaataa accttttttt atatcaataa ttccacataa
    15721 aacaagaaag ttaatcgtct ggtcaaaaga ttttcttaat gaatcgactg agttgtctat
    15781 attatcggca gtatcgatta ttggagtaat tgaacgatga agataatatg cgaaaagcag
    15841 gttggccgga atacttttct tccgtaaggt tgtaacacaa aactcaatat cttttttggt
    15901 aggaacctgt aaaggtgcct taaagttatc attgccaaca gttcctgata ttcgtttaat
    15961 tatttcggaa agcattttgt gaggcatatg cattagtttg ctaatgggtt caatggaaaa
    16021 aaagacattt gtttttgtaa tttcaagagt ctcaagcaga tacaaaaggt aaagtgatag
    16081 ctgagtgctt tcctcgacgt catatatcaa accatcggct gtttgcatct cgataacaga
    16141 catatgcagt ttttttaagt cggtttttct aaagtggttc tgctgagtaa gataaagacc
    16201 gttagatctt ataatatcag atatgtacag ggtattaacc agcaatgaat agccattttt
    16261 gacaaaaata tcctgtcctt caatgttttc ggtttctata tagtgctcca tggtgtcatt
    16321 gctaagcaag ataatggtag caaaagggta ggtgagttta aggttaattt ttatataacc
    16381 tttttcttca aattcagtaa gaatctgatc atattttgta agatctaaat cgttttgtaa
    16441 attattaagg ctgattattc caccgtgatt atatatggtt tttaatatct cttttttgtc
    16501 cttaattttt atagaggaac tgtcagatcc ttgccggggt ttgctgctta ttagctgctc
    16561 attgatatct ttagaggtga aatgattaac aatgttgtag ataggttcat aaagatacac
    16621 cttgtcaaaa cggttattta ggtgtttccg gtttttgaat atgtatataa gaagcttatc
    16681 ttttagatta tcgcaaacag actcaattgt cgcaatatcg aaacctgttg ctttttcaat
    16741 ttcactgaat gtgcgtccct cttcgtcggt agagataaat gaaaaaatct gcaactcgtc
    16801 tggccgcaat gatgaaagca cggaatttat tccgccaagt ttcatcaggg cagctgggat
    16861 ggccgatttt gatgtagatc cagaggcacc gattattttt tgtagctctt taatcttagc
    16921 cggatccatt ttttccagag ttttttttaa tttttccata aataaaaaat tgtagacagt
    16981 gatatcggtt tttcataaca aagacaagtt cggttaagat gagatcccag ataagctgct
    17041 accagttaaa ttacaaaaga acaaccttat taattaaatt atacttatga tcataattct
    17101 gaatggtatg aatagatcaa gtcaaataag tttttatcgc aagaggctct cttaatgtac
    17161 cgttatttaa aaattagaaa aaacgaaggt ctttacggtc ggttctcggt tatacgagga
    17221 agatcaatac ccttgtttta tataaaacag cttcttgttc tgatttttgt agcatattct
    17281 acaatttttt tagcagttac aacccctttt ttagaaaaaa actcaatgac cgtatatatt
    17341 atcggcattt ttatccttgt tttttataca tctaggctat tatttcatgc aatttatcat
    17401 ttatactatc gtccacgctc atttttaacc acagtcgaac gaataattat taatgattat
    17461 caccattcca taatgatcga acgtgacgat atagaagcga ttgtcaggac tttcaccaat
    17521 ctgcttttga tacgtacaag ccgtaaaact tatcttttcc ctttgatgct aattgataaa
    17581 tcacaaatac agacccttgt aaccagtttt actgatcttt ttccggctcg cacgcgaata
    17641 attgacagat tttttgaagt tgtacacgca ataacacttg cattgattat ggcagttcat
    17701 ataattcagt attgtgcaca gaactatttt attcctacag gatccatgcg caataccttg
    17761 attgaaaatg atcatatctt tgctgaaaaa atcagctatg gcattacaat ccctcaaatg
    17821 cttggaatgg aaagttcaat gaaactatca tggaccagaa caagagccat aaaacgattc
    17881 gatatcgtta ttttcacacc tccaagcaaa agctccgatt ttcatggtaa tgagtatatt
    17941 aaacgaataa ttgccttgcc gggggaacac ttttcgatac ggaatggatc ggtatatatc
    18001 aatggtaaaa aactttatga accgtatacc ataggtttaa ctgaatatct tggtgatgaa
    18061 ggtttggtta ttgatggagt tgttccatcg gactcgattg tggtattggg tgataatcgt
    18121 acggattcac aggatagcag aatttttgga tatgtgccga taaataatat taaggcgcgt
    18181 gctttttttc tgtattttaa ctctgatttt agttccggag tgagtacacg ggatagattt
    18241 tcccgttttg gttttatacg ctaattattg gattggttgt aatcaaggag actccccatt
    18301 accggtaaaa catttaaaat aagaattttt gtcttttcta ttatgctttt gatcttcaca
    18361 ggattgctta tagttagggt cgctgtactg cattttagtg accgaattgt attaaagagc
    18421 gatccgaaaa tagaaaacct ccgaagagga tatataaaag attcggctgg aaatatatta
    18481 gcaataagta ttgaaacacg ctcgcttttt gctaaccccg agaagattga agatccgaaa
    18541 aaaacagcct tacagttggc agagatacta cagatacgcc ctgaatatat ttattcgaga
    18601 ctaactctgc cacgacgctt tatctggcta cgaagaaaat tacccgaggc tacagcttct
    18661 aggatagaag ctcttaaaat aaacgggctt ggcttcaaaa aagagtttaa gagaatgtat
    18721 ccgtatgaga cactcgcatc gcatataatc ggatttactg atattgataa taatggactt
    18781 tccggtattg aattttctca aaatgattat ttgtcaggca cagctgatgt agaaaacgca
    18841 gatataaata gtctgaatta cggttttaac ataaatttga ctatagacaa gaatattcag
    18901 tatatagctg aaaaatattt aaatgaagct gtgacaattc ataatgccga gaggggggcg
    18961 gtagttatta ccgatgttcg taatgcccgt atactcgcct gtgctgtaaa tcctgcctat
    19021 aattcaaatt actatttccg atataccggt aaacagcgtg cttcgtattc tgttgtgcaa
    19081 tcatttgaac ctgggtcaac catgaaaatc ttttctgctg cggcatggct tgagagtggc
    19141 agggcggata tgaataaact ttatcatgga agcggtgaga tcgatattta tgatgttact
    19201 ataagaagta cttctgtgca tggttggata acaatgtttg acgcggtacg gctttcttgt
    19261 aacgtttcag ttatcagagc tatgcaggag attcgcaagg atgaacttga tgccatgcta
    19321 cgaaaatttg gatttggaag caaagtgtgt cgtgattttc ccggggagtc tccggggtta
    19381 ctgaggcctg ttaatgagtg gtccggaata tctaaggctt caatttctat aggtcaggag
    19441 atgtcggcta cttcgttgca gttagctgcc gcttttggcg ctgttgcaaa tggcggtgtt
    19501 tatatacagc cctctttaat tgattctatt gaactaaata atggacataa aattcagcaa
    19561 ttttatccaa agtcaaaagg gcggataatc agtcgtcaaa atggtgaaat tttgcgtcaa
    19621 atgatgaagg ctgttgttga gacaggtact ggaaaaaacg ctgctgtacc tggatacgaa
    19681 gttgcaggta aaacggggac agcgcagata tcgtcttcaa ccggtggata tcttgaagga
    19741 gagtatacgg cctctttcgg tgggtttgcg ccttatgatg atcctttgat agcggtggtc
    19801 gtagttcttg accgaccaaa aggtcaagta catggtggag aggttgctgc tcctgttttt
    19861 tcaaaggtgg tttcagaagt attaccctta atgggggcgg gtgcggcctt agtaactaat
    19921 attgatccgc gtgaaatatc ggcaaggtct ccgcattttg atggagaaca tatgcctgat
    19981 ctttatggtt atcgtttatc cgagttgaca ccattgtttg aacatattca aaaaaaatat
    20041 gctgttcaat ttgaattttc tggatctggt gttgtaactt cacagtctcc tgaagcggga
    20101 actgttttgc aaaataaaca aaaaatagaa ctgcacttac ggtaaacatg tcatattttg
    20161 aaaaaaacat aacgatatta caaaaaaaag atccggcttt ggttgtaagt ttaaaaaaag
    20221 caaaaacaga attcatttca gtatgtgata ctaaaacagg cgacaaagtg cctcttgtaa
    20281 ctcttaatga gggcaaacga cctgtcgctg ttcatagtaa atttgatcct gacagagagg
    20341 ccgcaagatt aattgacgga ctggatttga atacgcataa tctttttgta gtaataggat
    20401 ttggatttgg ttaccatgta cagaatctct tggaacgcat aaatggagat tctcttgtct
    20461 tagctgtaga gaaagaactt ggaatgctta aagctgcttt aaccgagaga gatctcacga
    20521 aatctttgtc agatgagaga ctgcttttac tggttgatcc ggatgaagat attattgcgc
    20581 aaaccttaaa aggacgttcc tcgcgcagcg ttgtttttgt tactcatcga ggttcgtttc
    20641 aaatagatca ggattactat tccaatgtta atcgtcttat gcgatcttac cttagttcaa
    20701 aagaggtaaa tattgcaacg cttgcgaaat ttgaaaaact ttggacctta aactgtgcaa
    20761 aaaatgcgtt gcaacacata tcgttgcctg gagcgaaaat attttatgaa aaatttgagg
    20821 ggttaaacgc gataattatt ggggctggcc cttctttaag gtattctctg gaggcgttaa
    20881 ggcaaaatca gaactcggtc gttatggttg ctttagatac atcttataaa attctaatag
    20941 aaaatgggat cactcctcat ttttgtcttt gtgttgatcc tcagcttatt aatgcacgtt
    21001 attttgaagg agtgcctgag cgtgatactg tattggtggc cgatccgaca gctcatcctt
    21061 cagtttttag actattcaag ggtccacagg ttgtttgttc gatgcctttt gatacaatga
    21121 agtggctcga agagtataca ggtgaaaaag gtgaggtggc tcacggtggc tcggtttcta
    21181 ctaatgctta cgatttcgca aaacgattag gtgttaataa tatttatctt gttggacaag
    21241 atctttcttt taccgaacgt ttagctcatt gccgaggatc ttacttggat gagatgatgt
    21301 ttgttagaat tgatagattt ttcaatcagg agatgattaa caggaggcag ttgaccgctt
    21361 cacctaaaat aatggttcct tcgattgatg gtgaaaaagt tcatactaat cagaaaatgg
    21421 ttattttcca gaaatggttc gaaaaacgta atgatgctca tttgttcaat agtagtgtta
    21481 acggaataaa attgaatgga attaaagata ttgaacttaa tgcagcattt actgcctgtg
    21541 agaataatgt gtctgaaatg gtaaaaagta tatatttaga agcatctatt ccgcatgaaa
    21601 caaaaattga aattgttaaa aaagtaagtg gaagagtagt actccttagg aacgaaaaca
    21661 atgtggttat ggagcgcctt gaaaatgctg taaatacagc agaacaacta attgaggcat
    21721 taaaaaaacg atctaagcag gttggggctc tcgttaccag acttgacact attgatgagt
    21781 ttatagcttc aaagtcatct gccaaatcga tgatagggat gtccatacaa cgaacaattc
    21841 atacaataac ggaagggtac gatacggatg aatttagtgg ttctgggcat gaaaaaattg
    21901 ccagaaaatc acttttcttg tataagagtc ttcttgaagg atgccttttc gctgaaaagg
    21961 tattaaacaa aagcgaaaaa attgcaaaaa catttatttg taattaaata tccatgattg
    22021 taagagtaca atttatggat tagttgtata atagcgctcg gatccatcgg attctgaaaa
    22081 ttgtttattt ggccatctaa taataccagg attagagact atatgaatgt cattttcgcc
    22141 tttaattggt ttctgaaaat gttgataatc ccttcgtctc caattgcctc cccaatacca
    22201 gccatgtttt ttaaaagcca gaactacagg gtgctcttcg tatagtgttc caggtttttg
    22261 agggtcatag gttccgcctt tagggaatgt tgtgccccat ttacgaaaag gattaatgat
    22321 gggatttatg tcaattgcgt gtccaaagct atgataagag agagtgttct tatagactat
    22381 attgcgataa tgaaaactgt agctgttccc ctcgggaact gtattaaatt gaacccattc
    22441 atacgaggag tgatctatgg ggatgcatcc atagataggg aagcgctcgc ggagtataac
    22501 agtaaagatt gcgtgaatat cttcagcatg gcgataatcg gctagaatct gtcctcggtg
    22561 aattcgattg tcaaaactgt aataaaacac ttctatcaat gcgagattat caacaacact
    22621 ttcagggcac tcttcaggaa catttgcgag agcttcatct agtgtcatat cacagtcgac
    22681 aattacacgc tggctaaact ggctgaagaa tttttcggaa cctatctgat actgcgcaca
    22741 tgaagagatc aaggcaaaac agacaaataa agtattgaaa ataatgtatt ttgatataaa
    22801 attattcata aagaaacata ttttttgcaa ccggtttatt gtcaaggtaa aaattaccat
    22861 gttgtgtttt ttgcagacaa agatatttga aaatcgttaa gaatatatgc tattttaagt
    22921 tgacatgcct aagtagcctt cattaaatga caaaaaaatg aaaggatgtg attatatggc
    22981 gagtccgcta attgtaaacg tggataacgg tgaccttgag aaagcattca agaacctcaa
    23041 aaagaagatg gctcttgaag gtatttttaa agagctgaaa agacgccgct acttcgagaa
    23101 gccgtctcag gaaaagaagc gtaaacgtga agaagctgag cgcagacgat taaaaaagat
    23161 cagacgcttg gctgctcaga acagacgctc ttcgagaaag tagtttcgac gatcactata
    23221 atccggaagt aatattccgg attatttaat taccccgcaa aattatgaca ctctttaacg
    23281 ataacagttc attggtgcct cttgccgaaa gatgtcgtcc acatacactt aaagagtata
    23341 ttggacaact tcatcttgtg gataatgaca agattctttt tcgaatgatt gaggagaaac
    23401 gccttttttc catgatactt tggggtccgc caggttgcgg taagactact cttgcccgaa
    23461 taatctcgga cacgctcaat ttggatcctc actatttgtc tgcagtttct gcaggtgttg
    23521 ctgatgtacg aaaaattata gaaaagggac gaacgaacag attgcatggt gttgcgtctt
    23581 tgttgtttct tgatgagatt caccggttta acaaggcaca gcaagactct attttgcagg
    23641 ctgtcgaatc tggtgatatt gtgttaattg gtgcaacgac cgaaaacccc tcattttcga
    23701 ttattcctcc tcttctttct agagctcggg ttttcaaact ggagccgctt aaaactgggg
    23761 agttgcgttc catacttgat aatgctatta aaaatgattc tttgcttaaa atatatgata
    23821 tcgctgataa tctgcgagat gaaattgtaa aggggggcgg tggcgatgcc agaaagatgt
    23881 ttaatttact tgagtcggcc tgtgctctat ctttggggca gactttaaca gctgatgccg
    23941 ttgagcaggc tgttgcggca tcttcatatt attatgatcg ctcgggcgaa aggcattacg
    24001 atacaatttc agcatttatc aagtcaataa gaggttcgga ccctgacgcg actatatact
    24061 atcttgcgcg tatgcttaat gggggggaag acccgcttta tatagcaaga agaatggttg
    24121 tacatgcggc ggaagatata ggaaacgcat caccaacagc tttaacaatt gctgtttcta
    24181 caatgaccgc ggttaaaaac ataggaatgc cagaagctcg tattgtactc tcacaatgcg
    24241 cacttttttt agcctcttct cctaaaagta atgcgagttg tatggcaatc gatgaggctc
    24301 ttgattttgt gaaaagtgat gtgtcacaaa ttcctatgca tattagaaat gctccaacaa
    24361 aacttatgga agaattcggt taccataaag gatataagta tccccatgat tatcccggaa
    24421 attttgtaca ggaaaattat tttccggaag aactcaagga taaggtcttc tacaacccga
    24481 gtgaaaacgg aagtgaaaaa tcaataaaag agcgacttgc tcagttgtgg ccaagaagaa
    24541 atcgcaaggg atgactagtc cttgttttcc gaaacgtctg aatcaaatac agctttatct
    24601 gaaaaatggg cttctttcgc agaggtaaat agtgatattt tgcagtcgaa aatattatca
    24661 ctgaaaattg ttattctgat gcggtatatc cctgggtcaa gtgaagttag cccgtcggaa
    24721 ccgctactaa tctgaacaga ttctgaactg ttccatgaat tatcgaaatt tcgacctagt
    24781 tcgatataag aggtataaat tgaatcgctt gaagacgtgt catatttttt ttcaatgatg
    24841 aaaaaaaacc ttccttcgac cgacgaaccg gcagcattaa taatttgtcc atttatactg
    24901 gcctttatct cggactcttt ttcattaagc atgaaaaaaa gttcataggt tggatgtgag
    24961 tctatttcgt tcaaggagac tgaaatgctg ctacttgtgg cattataaac agcttttttc
    25021 ccgaatgggg ccctttgaat caatgcacag cctgttatta aaagcagcat gaaaatattt
    25081 aaaagtttag ttgtcatttg acccccgttt tttacaatat ccagagttac tcagggattg
    25141 tcaaaaaaaa attgttgcct tcagcacctt ccttttgatt actagtaggc tctgctggtt
    25201 taacttttca aatattaaat gtatttgaat gctatgaaga atatagtcgt agcatgaaag
    25261 ccagtgtaaa taatagctct ctgtaaagat tttgattttg tggtattttt accagccagc
    25321 ttgaaaatgg agaaaacatg tatcttaaat ctatggaaat attcgggttt aaatcatttg
    25381 cagacagaac tcccgtcact tttgaacctg gtataactgt tgttgtcggt ccaaatgggt
    25441 gtggcaagtc caacattgtg gattcggcaa aatgggtact tggtgaaaaa caggcaaaaa
    25501 acattcgtgg aacacggatg gaagatataa tttttacagg tacggagcat cgtaaaccac
    25561 tgtctcttgc ggaggtctcg ctaaccctcg ataatagtac ccgtctactc aattttgact
    25621 ctgaatctgt tacagttacc cgacgtatct ttcgtgatgg tgaatctgaa tatctgataa
    25681 acaagtcacc agttagatta aaggatattg aacagctctt tatggatact ggtattggta
    25741 agacgtccta ttctgttatg gaacaggggc gtatcgatat gattttgtct tcaagagctg
    25801 aagaccgaag atatcttttt gaagaggcag cagggatttc acgatataaa gttcaaaagc
    25861 gtgattctct aaaaaaactc tcggatacta gtgaaaattt ggatcgaata aatgatatta
    25921 ttcatgagat agaccgggaa aaacagaata aagccaaaca ggccgaaaaa acgaaagagt
    25981 atatcaagtt taagtcggag ctgaaagagt ttgatttaaa acttcaagcc ctaaaatacc
    26041 gtgatttaca taagagactt tcaaagattg ttgaaaaccg tgataaactt gtcgaagaga
    26101 gcaaggctat ttcacagaag atttctggta tttctgaaga aaatgaacgt gacgaaaaac
    26161 gtaaaaacga gatacagttg cagctatttg agctggataa aaaaatgcat acttatcgta
    26221 taaaggttga agatattgat gataagcgtg aaaaaaataa taaactaata caggaaaata
    26281 gagaacaacg cgagcgtctg ttagctgaag ttgaagaacg tcgtataagc tataaaaatc
    26341 tgacagaaga gaaggctcgt aatcaaaaaa acggatcaga gattgaacag aaaatagctg
    26401 aagatcgaaa tcagcttaag atatttttcg agcgacgaaa gcaaaaaatt gaacagattc
    26461 ataataatcg cgagacaatt gataaaaata gactttttgt taccgatagt gaaaaaacat
    26521 taaaaagcct tcgtgatgag cttgaagtgg ttattaagca gcttattgat gcaatagata
    26581 agcgtaaggc tgaattgctg gattcggaaa cggaacgcca gagtgttaga gcaagcattc
    26641 acgaacaggt cgcagatata agagaaaaat tgtctgcggc gataaatgca attgaagagg
    26701 gtggagattg ctgtaacctt ctttcctcta tcgatataga taaactaaac gaatcaattt
    26761 caagatttga aagttttgag gatggtttta gatccatact ttttgacaaa accggtattc
    26821 acgcaagaaa agagtcgata gacaaaaaaa ttgctgattg tgcactccgg attgaagagg
    26881 caagggcgca gaatatctct cttgaagaac agaatgtaac attgcaaggt gaacttgaag
    26941 acgttaatga gatgattaca agaattgaaa aagatttatc ccgaaatgac aatgaacggg
    27001 tatggattga aaaacatgtt cagggacttg atcggcaaat tgaagatatt gaaaaacata
    27061 taaaaaatgt tcacactgaa ataaagcgtt ttgaagaaaa aacagagcag ttacttgccg
    27121 aaatcaaaga ttgggaagaa aagcttgtcg aatttagtga aaggagtgtt tctctcaaga
    27181 aaagaataga agagagttca cacaagcgtg aagagataga taaaaaaatc gttagtcgaa
    27241 agcaaagttc tgcccgtgac gctgaaaagc ttaatgaggt aaataccaag atttctgatc
    27301 ttgaaaaaaa atcggttgaa atagagttta aaaaaaattc ggctgaagag tatatttgga
    27361 cagagtatga actgaagcct gaatctatca agaaggtcaa ggttgatgag cttgaacttg
    27421 aaaagattaa tgagcagatt ggaaaattaa aaaaaagtat agaaagactc ggaccaatta
    27481 acaaccttgc cattgaggag tataatgatc taaaaaaacg tttcgactat tatagtgagc
    27541 aacgtgatga catcatgaaa gcacgtgaag atattatatc tgttatagaa gatattaacc
    27601 agacatcggt aaggatgttt gttgagactt ttgagcagat tcagaaaaat tttacagaaa
    27661 tattcaaaca attgtttgag gggggacatg ctgaagtaat tttagctgat cctgaaaacg
    27721 ttttagaatc aggaattgat attacagtac gccctccagg taaaaaacca aaaacaatta
    27781 atcttctttc gggtggagag cgcagtatga ctgcaattgc gttgcttttt tcgacatata
    27841 tggttaaacc atcaccgttt tgttttcttg atgagattga cgctgctctt gatgaggaaa
    27901 acgtaaggcg ctttttgaaa atgatgcgtc agttttctca aaaaactcaa tttataatag
    27961 taacgcataa taaaaagaca atgacaatcg gttcgtctat ttacggggtt actatggaag
    28021 agccgggtgt ttcgaagctt gtatctatga gattgggcag tgataattcg tcaaatcttg
    28081 cttaatgttg ccttgaataa cttgttttaa tcgtgtttaa agtgtatatt tatatctctt
    28141 ccatgagtta tgtctcaaaa aagtcttaac tcttttttat cttggtcgat atattaatat
    28201 gtctcatata ttggatcaat ttaaataatc gggccgacat taggaattat gcctattaat
    28261 tgattaatat ggatatacaa aaaaataaag tcttgacgta aaagactcgt ttgtgtatat
    28321 tgtagtgtta ggcaaaaagg attttgtttg ttcacaaagg agtgtgacta tgaatattac
    28381 agctgatggc attcttggtt ctgccagaaa aatcaatcaa aaacgaacct ctttatcaga
    28441 aacttcaggt ccgaagagta cagcgggcaa gtcggattct gtgcagattg agactcgcct
    28501 taacggccga cttgacactt tacaggctga tctgaaagcg acccagacat cacttacgcg
    28561 taatcagata atccatgctg gattgaaaga tcttgctgaa gatttccgta aggggggcgg
    28621 gcgtagtgca gagattattt cacaggtaac ttataatggc gaacaacctt taagtgagtt
    28681 gatcggtgat aatactctta atgaaaaatt tcttgatgag ttaatgaaag gcacagttat
    28741 aagaatcaat aaggatatag ctgatctttc gcgtatgcaa gtagagtcag aaaatatctt
    28801 tgcatcaagt cttatttctg atccatcatc aagaatgacc gacatgtttc aaaacaatgt
    28861 ttccggagaa agcatctcca gtcttaatgc tgacgttgtt atgagattaa cacgctgatt
    28921 gatgtttttt gttttgtgta aaagctggat tactagagga agcatggtaa ggaatatatt
    28981 ttttacagta atagttttgc tctctttctc tttatatgca gctcattcta tatccggcag
    29041 tgattatctt cttttccagt tgggagatcc ttctgtgttg cctggttcag gttgggttaa
    29101 ccacgctgat gccacacagc tgcttgaatg gggacgagca aaggggctga atcttgttga
    29161 ggatatggct cctgttgcta acagacgcgc ctcctttatt gccgcttcat cggcctcttt
    29221 acaggttacc ggtctaaaag ctcatgctac atatactctc tggatagatt ttgttaagtt
    29281 tcgttccgaa aaaaatccgg gaatttactc aaagcttcat gtttacgcgg atgggcgtca
    29341 gattgatgag cttgtatggg gaaagttacc tccagacact ctttaccgta ttgatttacc
    29401 ccttgattta acttatgatg gtgatgtggt tattaccttt aaagagtatg ccatgaatta
    29461 tggatattgg ggagtatggg atatggttct ggcaacgggt gacttgcctg atggatcata
    29521 ttttaaaggt gctgctccat ctgtttcgcc tgaggattct tctgtgagtg ataagggtaa
    29581 agtctctgat ttgaaagaaa gtagtgatga tagccgtatt gttgtagata aaacaaaaaa
    29641 agatattgaa aagcctgcca ctgtaactaa aaaaacacca gtaaaaccta agcctgctgt
    29701 acgtaaacca tcatcgaaaa ctaaacccaa aagcccgagt aaaaaaccaa ctgtgtcgaa
    29761 acccaaacca aaagacgttt tgccaaaaaa accaagtgtt aaaacaccat cggtgacttc
    29821 tccagttgaa ccgatagttc cttttgtaga cgaacccata gttgatcgcc cggtaattcc
    29881 ggataaacca aaatcccgtt gatttcattt ttaattattg ttttctttca agtttatgaa
    29941 atatttacgt tataggtcat ttataattta tttaaatatg gtaaattcaa tattttcttg
    30001 gattcttgtc cagtaggttg gccttgcccc tcgtaaaaat tcattcccca gctgttaata
    30061 aagtatggaa acccaaagaa gtgataaaca aaagaagcat ggaagcaggg acggatgaac
    30121 tccttaacac caaccataaa ctcatcgaag aaatccattg caagaaatag catgtacgac
    30181 aaactgtaac agtaaagatt ggcgatgtcc tttagttttc gctttgtgat acttgaaaac
    30241 cggatatgca ccgatttgta aattgtgcct ctttcgtgat acttgttcag agtgtcatta
    30301 catacgggat tcttagccag ttttttcgaa tacatatcaa gaacggtatt cattacatcg
    30361 gaaatatcca tatcatgctt tgcagcatac tcttcaatta tttcaagtct gcttacttcc
    30421 acatcgattg atgtcctaaa atctgtaatt tcca
//
LOCUS       DAOTGZ010000010        26365 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_3064
            NODE_2124_length_26365_cov_5.557849, whole genome shotgun sequence.
ACCESSION   DAOTGZ010000010 DAOTGZ010000000
VERSION     DAOTGZ010000010.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868088
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 26365)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 26365)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:22:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,785
            CDSs (total)                      :: 2,751
            Genes (coding)                    :: 2,732
            CDSs (with protein)               :: 2,732
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 12 of 19
            Pseudo Genes (internal stop)      :: 5 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..26365
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2124_length_26365_cov_5.557849"
                     /isolate="AMR_MDS_3064"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..462)
                     /locus_tag="P5123_01580"
     CDS             complement(<1..462)
                     /locus_tag="P5123_01580"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRX14985.1"
                     /translation="MKNLFKFLALILSAMIILSFAGCSSDSDSATKLQTENVDDVDPA
                     AEKPENDSNENTDTDDDELEKAIEEINSKNSAATVINETGVGDTINYSDMSSLDNDPT
                     GLHITEDYDGDGIPNASEIVTNPYVADYPRIVTRISPPIIMELQPTTTTEVD"
     gene            complement(847..1830)
                     /locus_tag="P5123_01585"
     CDS             complement(847..1830)
                     /locus_tag="P5123_01585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014253469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 43 protein"
                     /protein_id="HRX14986.1"
                     /translation="MKSRELATHIYTADPSAHEFNGRLYVYPSHDRDIDVAYSDEGDE
                     YLMEDYHVLSMDGPDAEVIDHGIALHVDDVQWASKQMWAPDAHEKNGKYYLYFPARDK
                     ENNFRIGVAVGDKPEGPFVPEKDYIKGSFSIDPCVFKDDDGSYYVGFGGLWGGQLEKW
                     QTGSFVADAEGPKGDEPALCAKIAKLTGDMLQFDGGVHDIQVVDKDGKPLTAGDEERR
                     YFEGPWIHKYNGKYYFSYSTGTTHCIVYATADNVMGPYTYQGVILTPVEGWTTHHSIV
                     EFKGKWYLFYHDCERSKGINNKRSVKMAEITYNEDGTIQTLVPDESLIGKR"
     gene            2166..3041
                     /locus_tag="P5123_01590"
     CDS             2166..3041
                     /locus_tag="P5123_01590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014269762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VIT1/CCC1 family protein"
                     /protein_id="HRX14987.1"
                     /translation="MKISDETRKIVLSMQINELTEYHIYTRIAGFVKDVKNQKILITI
                     GNEELKHSEIWSEITGKQVKPKKIKILFFTFIARIMGYTFALKLMENGEDDASEIYNK
                     ISTEVPQAKQIAKDEDHHEQQLMEMLDEERLQYVGSMVLGLSDALVELTGTLAGLTFA
                     LQNNRLVALSGLITGISATLSMTSSEYLSARSDGHHNPLKSSLYTGSVYLITVILLVL
                     PYLVLPQNAFIPALAILLATVVIVILFFTYYISVAKDLPFGKRFAEMAGISLTVAAIS
                     FAIGLSVKYLLGIDI"
     gene            complement(3063..3587)
                     /locus_tag="P5123_01595"
     CDS             complement(3063..3587)
                     /locus_tag="P5123_01595"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRX14988.1"
                     /translation="MVSDLSAELKRITFVTRAIYVAMLFTYPFYYFLLATTVGLHDVS
                     DNKDIWTLPVIVTAVGVFVLSGLGAYIAHSVYSGAEFYEGRFGKFFKVRIKQNGGIQP
                     SSIFALYVVALTIIQASSVLLFALSTVLQNAWYYQFFIMPVIFFFAISYPKTSKLEEL
                     IKGAERTVQKPGQE"
     gene            3734..4258
                     /locus_tag="P5123_01600"
     CDS             3734..4258
                     /locus_tag="P5123_01600"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRX14989.1"
                     /translation="MKAIKFFTYFAHIHNILGGLLIVAAIFIFLISSSDYAAQNIILI
                     AFNLAVAITCFTGSIMTLSINRYPVGITILGTLTALQIFSFATNNILVNINLIPSFGV
                     IFNMGQGAEKTIEFFFQIIPHALIDTAYPAQSAFGISIVPLILLFIIFPAYKRLKAEL
                     EELHKERLKAEISK"
     gene            complement(4260..4922)
                     /locus_tag="P5123_01605"
     CDS             complement(4260..4922)
                     /locus_tag="P5123_01605"
                     /inference="COORDINATES: protein motif:HMM:NF013045.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA];
                     GO_process: GO:0009451 - RNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pseudouridine synthase"
                     /protein_id="HRX14990.1"
                     /translation="MNTVSVIYQDEHIVAVNKPSGMFVHRTTLADESVFLLQTVRDLI
                     GKHLYPVHRIDRPTNGLVVFALSSESAASLCDNFRYNRIEKKYLAVVRGWVENGTIDY
                     SLLNERGNKRNEAVTDYLLKQKKEYPFAVGKHQTSRYSLVEVSPKSGRMHQIRRHFKH
                     IFHPVIGDTTYGDGTHNRIFREQFSRHNLQLQAVSLSLSHPDTNKFLTISLPTDMQLT
                     LP"
     gene            complement(5008..5817)
                     /locus_tag="P5123_01610"
     CDS             complement(5008..5817)
                     /locus_tag="P5123_01610"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRX14991.1"
                     /translation="MRKTSMFLSMTLAALMITGSLYSETYYTLRAGGGIAGGGDKIMG
                     RDYDYVSSDGGDGPAIAVSLSASLLNVRSAKNSLFFGAQITANMSEIPVSSDSEAPVS
                     GSYDVYWTQNELDAKYLYSISRTVGVFVGPRLVMNLSYGEYPLTNTESEYSDDFFYRD
                     NLAGITAGLNLRKHLFGTSFIVSTIGYGALIGVNSTTYKNETVDGDQTNMMNSLSLGY
                     AYYSVDSKTMFDVSVVAVFYLRGTPIMTSFDDEIEAGRITLMVGVTRPFKN"
     gene            complement(6026..7348)
                     /locus_tag="P5123_01615"
     CDS             complement(6026..7348)
                     /locus_tag="P5123_01615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008507220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015205 - nucleobase transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NCS2 family permease"
                     /protein_id="HRX14992.1"
                     /translation="MEKLFKLSANNSSVKNEIIGGVTTFLAMMYIIVVNPAILKEAGL
                     PFGAVLTATVLVSAFSSIAMGLYAKSPIAIAPGMGLNAFFTYGVVIGMKVPWETALGA
                     VFWSGIVFIILSVFNVRTHIVKAIPKQLRFAVAAGIGLFITFIGLINGGFIVDNPATL
                     ISFKGLNAVTVTFAAGLFLTAVLVVKRIKGALIIGIAATTLLCIPIGRFYGDGSWISP
                     VQSSMLVQYNGFFASPDFSLLFKLDFIGSIKLAMWPVIFAFLFTDMFDSISTFIGVAE
                     AADLLDKEGEPLHVKECLIVDAASTTVSGLLGTSAGTSYIESATGVEEGGRTGLTAVI
                     TGLLFLPFMFLSPLLSVVPGVATATALVLVGVFMMKPVSLINWTKFDDAIPAFLALVL
                     IPLTYSITQGIVWGFLSWTVIKLLTGKHKEVTPTLIVIDILAVLALVL"
     gene            7695..8075
                     /locus_tag="P5123_01620"
     CDS             7695..8075
                     /locus_tag="P5123_01620"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRX14993.1"
                     /translation="MNLYPSMPPVIKRKRSLFTSLFYTLSLLFIFGTAIFILYTNSRA
                     AKSAIKTEFNDINGKIISKFTLGEDISPKRYLVIQQPNNMITMLTVETEIYNNLQLNS
                     PVIYSYSSSNGSLKLKKIRQNDSN"
     gene            8224..8694
                     /locus_tag="P5123_01625"
     CDS             8224..8694
                     /locus_tag="P5123_01625"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRX14994.1"
                     /translation="MSDNNKSKTDEKLKERIANLSQNLETDELQSIVRQMEILIHNRE
                     VVREFQERKAARKLARDNSTEEENLDVKIIESEDGKSFRLFVGNFPKMFMLDEMRRMV
                     KLCHSSKTPEEGAKKLFVWINNNRKDFFMDIQTNNKEQVFLNIRNCIVERYTVK"
     gene            complement(8699..9103)
                     /locus_tag="P5123_01630"
     CDS             complement(8699..9103)
                     /locus_tag="P5123_01630"
                     /inference="COORDINATES: protein motif:HMM:NF033749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriohemerythrin"
                     /protein_id="HRX14995.1"
                     /translation="MAFIDWSDEYSVSVDIFDKEHQSLIAIINELHQGLTSGFGIKEM
                     TYILDRLLEYTSKHFYHEEKEMHAYAYPDFDVHKKAHEQLVADVKKYKDRLESGEQAF
                     SLELLAFLKKWLVNHILGTDKKYSKFFAGKNI"
     gene            9485..12214
                     /locus_tag="P5123_01635"
     CDS             9485..12214
                     /locus_tag="P5123_01635"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRX14996.1"
                     /translation="MKISDIIQKDSDRVFVTDFGCFIIFTGNTLDDSQPFIRIGNWMD
                     MPVELIPLVENIIITDDMPGNPAQEQFNIDINTQSSNRYIGSHYTLKRFLDFQKIFGL
                     DLKNVTIVDVEKDIPEISSRSLSDRESFIGIFYRDGNFKISHNKKDLFDLQKLLEKTY
                     NTNRVHDDLYENTKSNRAHFGSGFTIADSLPLFFDQGSYFLYMLPQNYQQVFDQYGIN
                     PRKISAIAHPSANYLNLTAFLRWINHSSSKLTILSDDKPQMNIIKKLFSRANLTCRSF
                     SMSKTEVTPGMIVNEINGSGNLEVTFNSETSQTVNFLYFNNSTDIDKIIKQTADFFVI
                     PYSVFECCNLVLKSTKTPYFILEDSNPNFSKLSSLTHSALFKDVNYQVIKYESSNDCL
                     DFFNSIISNDIVNALMNPDNPDNNKMINDFFENESSPFDRTNCISLIKYLIHISTNRK
                     ISSYLKKLIQNYAVQNSVHNLKAMDIAFSSKIIVADEGVFTVYTPISRSNRPKNYIPM
                     VPVQIPTDESSYKNSIEYRDYAAKIENDRKRLHALVQLYLGNGKTKPVRSLKKAINER
                     KEIYKQNILSREDINKLHINQIFDDNNQTDVSKTKGIKPLFTKIIRRIKTAVFNNQRY
                     NKYVQNKNEYAAHKELKGHHSSLIQRIIHPFWYSSPIIFFRSLNRILTAFLIIVLVIV
                     LFSLYWKAYQKKPFIDTIAELISRGSKTTASENITSSQNDESFDALANNNSTDDPEKN
                     GTLITDSDHNTEATNLEDNTEIKPVTPPTEISDLEERLEGNDNEEIIEKFKIKITDTE
                     ILYYLNEIAVINGYRPLGPDEVKGKNPHWIFPNSIFTLPDGERFIVRDGDSMWKIAEL
                     KLTKMSIEFHKAVESIKSFTGTKRPDELFEKANKHSFGDAQKNILKLLEDRFK"
     gene            12359..12952
                     /locus_tag="P5123_01640"
     CDS             12359..12952
                     /locus_tag="P5123_01640"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRX14997.1"
                     /translation="MDSTDKNNYYIEPVEIEIYLKKAGTVKTIIKDLYIELVDEDPLP
                     ENAIKIFDYFREKDQPIDLIELQNRFPEMILPVIESYYRFIDLYEKLSMHFRSALSGS
                     IDSLRQALYMTELLMKYEPTLCSLKLIGDFSTYNLNYLIRKLNQNNEEFLLEDSTVEY
                     LIKRHRSKHENDEDGSNKEFEKLVELWQYNLKEKPIL"
     gene            complement(13064..13303)
                     /locus_tag="P5123_01645"
     CDS             complement(13064..13303)
                     /locus_tag="P5123_01645"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRX14998.1"
                     /translation="MDDLHKRMLNKALGRDAFQQQAGGGGRGVGELDQLVRQSVSDLY
                     MALIESQTDPAQAIDLLAQHVAMMVEMINEFKKAK"
     gene            complement(13324..14541)
                     /locus_tag="P5123_01650"
     CDS             complement(13324..14541)
                     /locus_tag="P5123_01650"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRX14999.1"
                     /translation="MKKKLLSAIILFYLVPCFGYTDQTTTERIIKENKLFVEFLNICL
                     SNFSDKDSEKLDTFYNVYQSHFNGEVAFLQSDYHRAFKDVYKSQDANVHLYNKVLNEY
                     YLDESKIVLDRLAPGIIKSKNSAARQYLTLGYRDRALARNVQMVGNASRPHLRSHKIY
                     AFIEAIKYSRRSMRYALLALFESQDIETKKLIYKHLFEIERENNNPFYNRFLSKDDKA
                     LVAEINRDYEDYENEYGPKLEAELKRYEETGKIPDRTGGSASGTELDSDPAGGGVSVA
                     PGNPEAVDYLYENKIERRLRFRQEQRVANYVRNGEFYLANEVIVKYVNDFNFKLIAAT
                     IEVMRAREDKVKYKLDYDKLYIQHLDNYSRLARESVLDSFADRVRVVDDITKEGGEKK
                     DAKDPVLSDDPDA"
     gene            complement(14713..16515)
                     /gene="pepF"
                     /locus_tag="P5123_01655"
     CDS             complement(14713..16515)
                     /gene="pepF"
                     /locus_tag="P5123_01655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004075352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oligoendopeptidase F"
                     /protein_id="HRX15000.1"
                     /translation="MNSKAIPNRSQIEESDKWNIKKVYESIEQWQKDYSEIEKMIEKY
                     DSLKGTAGNSVNKCLELIEFDLEVSRKIDKVYLYAHLLNDQDKTNDSGETLYQRAVTL
                     FTKISEKSSFIVPEIQSLDDEILEKYLESQELTQYRFYLEKIIRFKPHTLPKESEEIL
                     AMSGEVFSTAGKIFSQLDNADLRFGDIVNGKGENIELSHGNFISLLHDCDAAVRKNAF
                     HQYYDAYDNHKYTLASSLSFSVKKDWLKARLRKYSSCREAALFTDNVPIAVYDNLIES
                     VKKSGDVIKRYMSLRKKALNVKELHFYDTYVPIINDVDFCMGYKEAVETCVAAVSPLG
                     ADYASIMKEGLLGGWVDRYENRGKRSGAYSSGCYDSDPYILMNYDKTNINSLYTLMHE
                     AGHSMHSYFSRQKQPYVYSDYTIFVAEVASTFNETLLSDYLLKKYADNKKMKAYILNR
                     EIDNIRGTLFRQTMFAEFEHLSHAEVEKGNPLTIDKFREIYRGLLEEYFGDSIVIDDT
                     LELECLRIPHFYSAFYVYKYATGISAAVSLASGVLQGKQGACDNYRQFLTLGGSMYPI
                     EELKVAGVDMSRPDAVNSTLSRFKELVLEMESLI"
     gene            complement(16539..19190)
                     /locus_tag="P5123_01660"
     CDS             complement(16539..19190)
                     /locus_tag="P5123_01660"
                     /inference="COORDINATES: protein motif:HMM:NF015714.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ComEC/Rec2 family competence protein"
                     /protein_id="HRX15001.1"
                     /translation="MLYKNFFLTAGGLLAGIALYTFTSVPIIYALLAFCISLPICMFI
                     LYCELKWKEFSLIFSILGFVVFGMASGYMRMHIAMQPVNIDLDAYISNPEGTIRIYGT
                     VTGEPILRNNVETELLLSVKKVYDFTSNKWVDTDNIKVLVKILKNDTTKASFFETLSD
                     NEIAGTYLDINAQKISPAAPLNLSEFDYINYMQKNNIGVLLSCGSRDVGFAKRADFFS
                     LKDIALKLKEYFIQTYRRTVPSPESRIVTSVILGTRRSLDMIEYEEEKITDIFKKSGV
                     TVFIENSGLHVVILSLMLYTLFWGIGFKTRHLAPAIIGLILLFTLLSGARPAAVQALT
                     VTAIAIAVFSYLHYNIRKSAIIGLSMTVIVICLYNPFSLFTFQFMLTFGSILVLIGLT
                     PPVLRWLNMLHGFTLIIFFIYYLLLVYLINIGAQYFFRPEYLIVIAGLAWGIVRIGRL
                     LNNRFPLFWNLSFISFPVPIKAIVAFLLSYQAGVLIPLSVWDTGLFPTSSLVLLWAAV
                     PVIGMILFLGTITGLTGLIPFFGEWIALPLGGASWLLSRFLLIITHAGSDFLKSPTLP
                     KPDFSWIIYYFIILLLCLYLFKLLPYFKAVFYKIDNHLNSFLLRALPFLAALVILLTP
                     LVISITAVEREMQMIVYHTQESPVVSLITYTGDSALIISPAESINTDSVSSGVRSMQS
                     TSISTLFTSQVNSQIISDIATLKKQGFKPSTLLLPKSLANETNSFAGLPKEIEKAFVS
                     FYNLTPSSRIIFGSEGFEEEYMILSIFGQRVLLLNSLKGFDINLIDDGSGFSLIVIPE
                     INSEQEHDFAIDIINGTKTDQVALINGMSKFSKMFMQNLSVREIDLYRTGSNGALFFN
                     ISQNHFTVDTLSEKRKREQIVIPALFD"
     gene            complement(19190..20986)
                     /locus_tag="P5123_01665"
     CDS             complement(19190..20986)
                     /locus_tag="P5123_01665"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRX15002.1"
                     /translation="MSYKRAVIVVHGSGLLFHETLDKLIDLPQIEVCYSGGIYYFKSS
                     DRLIIRRLLSFPRESKLITSYQKLLIAKMLKASLSPVTAPKGAFYEYNEEELHYFFGD
                     FGLPFSYFERKYRTKLLEQRSLRELDRTLLKMEIMKLSLLDTEGVMLHEDEIRKKYST
                     DIEFSSIYELVNSIQVRYESGCSADSAFAAALTAYKLKHKKNNCKYGKDYVYGFVQYY
                     EGLRSLEKFSPASLYINDIPPHVIPSFEDDLAYLKEKNVVLQEYNVQECRQGEDELIK
                     NLYHKGLIKKTNVFNHKRGLLSTESVMESFAKETIPLKKGTQTLLKILRSDGDRTSKE
                     FDMIEELLGSPISIIEIIETVAKSVSSDSLIRSIRGKKWFTDIQKLNDYHASIEDKLL
                     ESVFELRCSVPYDNENYLFEKDFSILIAKSVKPGRKRPALTVGEAAFYYRRKFPQASY
                     LLYCFGSGLIFFRWLKYRSVALNPFLLMSRIGIDGDGSETASPTCRARDNSLYPRQLL
                     GNVSSHNFKRFVSYLTLLIRDKMGCKVILKDVSEQIDVLHKQRIMKVALFFIICLILG
                     LSVLVTNEQYRFEEIEETNRDFYNTAPIPGDQ"
     gene            21183..21716
                     /locus_tag="P5123_01670"
     CDS             21183..21716
                     /locus_tag="P5123_01670"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRX15003.1"
                     /translation="MKEYSEKQILNAKDPAQFIDRCVNSNVSRGKKIVLGRKWREKTG
                     FTMEDIEYARNRHPYWKEKKMAGWRERNEKRWNAYNFTSKESLESDYWTKNNFANLRE
                     YIKKDKKNSKGVYNAKDRELAEFFRTSIPSIQHMRRKSNMVTKIMGKRKIKDTVKNRL
                     DFMQLGETTLREMLNKK"
     gene            complement(21775..23592)
                     /locus_tag="P5123_01675"
     CDS             complement(21775..23592)
                     /locus_tag="P5123_01675"
                     /inference="COORDINATES: protein motif:HMM:NF012711.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="long-chain fatty acid--CoA ligase"
                     /protein_id="HRX15004.1"
                     /translation="MYFDDYQSLPQIFFLNAEKNPDKPLLSVKVKGQYKDIKWDTVKE
                     EVYVIASYLASRGVSKGTKVAVYSENRPEWWVYDLAVLSLGAITVPLHAVSAVGTVEL
                     ILHKSGAQVCFVDTEERLNTLQLMSRKLPLLKEYIMFDKIETKKKNIKYYGDLIEKKL
                     KPADYKRVDAAVKKVKREFTATLIFTSGTTGEPKGVELTQHNFLSNTGQIEEAMGKYL
                     DETDAFLSFLPLSHVLERTAGYYYPMTTCKKVYFAESITTIQQDLVATRPTILVSVPR
                     IYEKMHSAIVGKVTKASPVKKAVFKFSVAMGQKAVPYHCKKKPLPFYLKKPHAIAEKL
                     VLSKLRVALGMDNLKFAISGGGPIAYSDMTFFVGMGVTVLEGFGLTETSPVTNLVLPD
                     DMKLGSVGKPFKNTKIKISDEGEILIKGPQVMKGYYRNAKATKEVMTKDGYFKTGDIG
                     IVDEEGFLYITGRIKDIIVTAGGKNISPQPIEQILRTSRLIEQIAIIGDNKKFLSALI
                     LPDFEELSARLAARGVGSGMNKKDIVKQPEAIALMREEIDSLTASMSRVELIRRFTLM
                     SGEWTIDGGELTASLKIKRNVIQEKYKEVIDKMYLDDEE"
     gene            23923..24462
                     /locus_tag="P5123_01680"
     CDS             23923..24462
                     /locus_tag="P5123_01680"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024917.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HRX15005.1"
                     /translation="MERRYLKCRELSQKRAIEIITMINSIWPPKDILSEIQYSERYIA
                     KNENNDLILYLDSGKIFAHAMSFIREIKTDYGLMKILAIKGIAVKPEYRDKNFGKKIV
                     LDAFSSLDTKNLNLSLFQTGVPSFYKKLGARTVKNRFINSLNNEDPQSNPWWDKEVMI
                     YPGSAEFPDGVIDLLGSGY"
     gene            24470..24898
                     /locus_tag="P5123_01685"
     CDS             24470..24898
                     /locus_tag="P5123_01685"
                     /inference="COORDINATES: protein motif:HMM:NF021612.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction resolvase-like protein"
                     /protein_id="HRX15006.1"
                     /translation="MFFSQPEIIAIIVLITALLVLIFIAGRKNGSSSNEKRWQHQLKF
                     IKKDIADSSRSVIRGQVSEQIAPFLPGFPFSPSGCRFIGKPVDFIVFNELEDPDKCSI
                     ILVEVKTGSSSLNRNERAVKSAVKSGRVHYYEYRFNGADN"
     gene            25023..25493
                     /locus_tag="P5123_01690"
     CDS             25023..25493
                     /locus_tag="P5123_01690"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRX15007.1"
                     /translation="MKEDNPNIRTCVSVQVDLLDKIIDFSDTNNIPVSDIINVLLQKQ
                     VEKLASNPQNREGAMKYQDRGLQYKPVHLKISYIQYRRLRDIMFLFRITLSYMLFLAM
                     QYFDTYLNEFKNNINDKFPPVCHCSILTFFDSLPVYAHYWGIPPAEHTISIPAG"
     gene            complement(25563..>26365)
                     /locus_tag="P5123_01695"
     CDS             complement(25563..>26365)
                     /locus_tag="P5123_01695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012389299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="TCP-1/cpn60 chaperonin family protein"
                     /protein_id="HRX15008.1"
                     /translation="RRKAMMEDIAILTGGQVISEEIGRKLDSATIEDLGQAKKIIVEK
                     ENTTIIEGIGKDTQVKDRIAQIKKQIEDSSSEYDTEKLKERLAKLAGGVAVINVGAAT
                     EVELKEKKARVEDALSATRSAVEEGIIPGGGLTLLSTQKAVEEAAKSLDGDEKLGAMI
                     IFKAVEAPMRQIAVNAGLEGSVIVEKAKSEKAGIGFNAADLKWENMIEAGVIDPAKVV
                     RSALQNAASVSGMLSTTEVLITDAKEENDNMPMGGGMPGMGGGMPGMM"
BASE COUNT         8738 a         5365 c         4358 g         7904 t
ORIGIN      
        1 gtcaacttcg gttgtggttg taggctgcag ttccattatt ataggcggcg atattctggt
       61 aacaatacgg gggtaatccg cgacataggg gttggtcact atctcgctgg cgtttggtat
      121 gccgtcaccg tcatagtctt ccgttatatg caggccggta gggtcgttat caagagacga
      181 catatcggaa taattaatgg tgtcccctac tcctgtttca ttaataactg ttgcagcact
      241 gttttttgaa ttaatttctt ctatagcttt ttccagctcg tcatcatcag tgtctgtgtt
      301 ctcattactg tcattttccg gcttttcagc agccgggtcc acatcatcaa cgttttcggt
      361 ttgcagtttt gttgccgaat cagaatcgga actgcaacct gcaaaagata aaataatcat
      421 agctgacaaa atcagcgcca agaatttaaa tagatttttc ataactcctc ccagtaattt
      481 taatttattt atatttatta tattttttat tgcacatgcc aattagctga ttgttgttga
      541 cgagatatgt tttacaggca agtcttagga tagcttgcct attaatacgg atttttttat
      601 attttgttaa atttttttta ttttttttac gttttttcag atcctaatta agaggtaaga
      661 tttatccgat acctgacttt tacccacatc tattatcttg caaatgtcta acactaagcc
      721 cctttgggtt gggggcatat agattaagat ttgctggcag aagtcaggtc tgacacgctg
      781 ggcaaacaaa aagccggaca gttatctgtc cggctttcaa aattccttaa cataataaat
      841 cgctgtttat cgttttccga taagagattc gtcaggtacc agagtctgaa tggttccatc
      901 ttcattgtat gtaatttcgg ccatttttac actgcgcttg ttattgattc cttttgaacg
      961 ctcacagtca tgataaaaaa gataccattt acctttaaac tccacaatag aatggtgagt
     1021 cgtccatccc tctacaggag taagtataac cccctgatat gtataaggcc ccataacatt
     1081 atctgctgtc gcgtacacta tacaatgcgt tgttcctgta gaatatgaaa agtagtactt
     1141 gccattatat ttatgaatcc acggtccttc aaagtaacgg cgttcttcat caccggcagt
     1201 taacggttta ccgtctttat caactacctg tatatcatgc accccaccgt caaactgaag
     1261 catatcgccg gtaagtttcg ctattttggc acataaagcc ggctcatctc ccttagggcc
     1321 ttccgcatcg gcaacaaacg aaccagtttg ccatttctct aattggccgc cccataatcc
     1381 cccaaatccg acataataag atccgtcatc atctttgaaa acacatggat caatactaaa
     1441 acttcctttt atgtaatcct tctcaggcac aaaaggcccc tccggtttgt cgcctacagc
     1501 cacaccaata cggaaattat tctctttgtc ccgagcagga aaatacaaat agtatttacc
     1561 gtttttctcg tgcgcgtccg gcgcccacat ctgtttggag gcccactgaa catcatcaac
     1621 gtgcagagct ataccatgat cgattacctc ggcatccggg ccgtccattg aaagaacatg
     1681 ataatcttcc attaagtact catcaccctc atcagaatat gctacatcaa tatcgcggtc
     1741 atgtgaaggg taaacataca gtcttccatt aaactcatga gcagacggat ccgccgtata
     1801 tatatgagtt gctagttccc ttgatttcat ataaaactcc tttcaaatta ttttcgtatt
     1861 ctccgacttt ttcaaatgat tagaatatcc cctgattgtc aagatatctt tgatactcct
     1921 gattgttgaa agatataatc aaaaccatga tgtttcaagt tttttcatat aacaagggtg
     1981 tctccaaaaa tttacaaatt tacatattat atcaatatca aggttgactc tcttattaaa
     2041 ttagtgacaa tagagaatag catgtgtaaa ttatcccaag attatccagt aaacgacccg
     2101 aatttaatgg aaaaaactaa taccggcaat tcggcaatga caccatgaaa cctgatggag
     2161 acaacatgaa aatatccgac gaaacaagaa aaatagtgct atcaatgcaa atcaacgaat
     2221 taaccgaata ccacatctat acccgaatcg caggatttgt taaagatgtt aaaaaccaga
     2281 aaatactcat aacaattggc aatgaagaac tgaagcacag tgaaatttgg agcgagatta
     2341 caggcaaaca agttaagccc aaaaaaataa aaatactttt ttttactttc atagctagaa
     2401 taatggggta cacttttgct cttaaattaa tggaaaatgg cgaagatgac gcttctgaaa
     2461 tatataataa aatttcgacc gaagtgccgc aagcaaaaca gatagctaaa gatgaagatc
     2521 atcacgaaca acaactcatg gagatgcttg acgaagagag actgcaatat gtcggctcta
     2581 tggttcttgg ccttagcgac gcgttagtag aattaactgg cacccttgca ggattaacct
     2641 tcgctcttca aaataacagg cttgttgccc tttcaggact tattactgga atatcagcaa
     2701 ctctttcaat gacatcaagt gagtatctct ccgcacgttc ggacgggcat cacaatccac
     2761 taaaatcatc actatataca ggttctgttt atctgattac ggtaattctt ctggtgttac
     2821 cctatcttgt tcttccgcaa aacgcattta ttccggcatt ggcaatactt cttgcaactg
     2881 ttgttattgt aatactgttt ttcacctatt acatatccgt cgccaaggat cttccttttg
     2941 gcaaaagatt cgcggagatg gcaggcataa gtctgactgt tgccgcgata tcttttgcta
     3001 ttggtctttc ggtcaaatat ctactgggta tcgatattta gagaataaag ttttacaaat
     3061 cactactcct gacccggttt ttggacagtt ctttcggcac ccttgatcag ctcttccagc
     3121 ttgctggttt taggataaga tatcgcaaag aaaaagatta caggcattat aaaaaactga
     3181 taataccagg cattttgcaa gacggtactc aatgcaaaca gcaataccga gcttgcctga
     3241 attattgtca aggctacaac ataaagtgcg aatattgatg agggttgaat tcctccattc
     3301 tgctttattc tgaccttgaa aaacttgcca aaccgccctt cataaaactc tgctccggaa
     3361 tatacagaat gagcgatata tgcccctaaa ccggataaaa caaaaactcc cacagctgtc
     3421 actataacag gtaatgtcca aatatcttta ttatcagaaa catcatgcaa acccacagtt
     3481 gttgccagca aaaagtagta aaacggatat gtaaaaagca tcgctacata tatagcccgg
     3541 gttacaaatg ttattctttt tagctctgca gatagatcag aaaccataac tgttctccat
     3601 attttttttc tactctacaa gaccttagtt ttcgattcaa ctctttttaa agtgaacttg
     3661 ccaaaaaaga attgaatact ctttcggttg tataaaaatg gcaaataaac tgtttgttaa
     3721 taaggaagcg acaatgaaag ctatcaaatt ttttacatac ttcgcacata tacataatat
     3781 tctcggtggt ctgctaatag ttgccgccat ttttattttt ttgatcagct caagcgatta
     3841 tgctgcgcaa aatattattc ttatcgcctt taatctggct gtagctatca cctgtttcac
     3901 cggaagcata atgacccttt caattaatcg ttaccctgtc gggataacca ttttgggaac
     3961 acttacagcc ctgcaaattt tttcttttgc tacaaataac atattggtta acataaacct
     4021 tattccatcg tttggtgtaa ttttcaacat gggacagggt gccgaaaaga caatagagtt
     4081 ctttttccag ataatccccc acgctttgat tgacacagcc tatccggcgc aatccgcttt
     4141 cggcatcagt atagtaccac taattctatt atttataatt tttccagcat acaaaaggtt
     4201 aaaagccgag ctcgaagagc ttcacaaaga gagattaaag gctgaaattt caaaatagac
     4261 taaggaagag tcaattgcat atcagttggt aaacttatag ttaagaattt attggtatcc
     4321 ggatgtgaca ggctcagaga aaccgcctgt aattgcagat tatgcctgct aaattgttca
     4381 cgaaaaatcc tgttatgcgt cccatctccg tatgttgtat caccaataac gggatgaaat
     4441 atatgtttaa aatgccttcg tatctgatgc attctacctg acttgggcga cacttcaaca
     4501 aggctgtatc gtgaggtctg atgttttcca acggcaaaag gatactcttt tttttgttta
     4561 agcaaataat ccgttactgc ttcatttctt ttattgcccc tttcgttaag cagggaataa
     4621 tctatcgttc cgttttcgac ccacccacgc acaaccgcca gatatttttt ttctattcta
     4681 ttatatctga aattatcaca tagcgaagcc gccgattcgg agcttaaagc aaaaaccaca
     4741 agtccgttag ttggtcggtc aattcggtgt acaggataaa gatgtttacc aatcagatct
     4801 cttacggtct gcaatagaaa taccgattcg tccgccaagg ttgtgcggtg aacaaacata
     4861 cctgaaggct tgttcaccgc cacgatatgt tcatcctgat atattacaga aacagtgttc
     4921 ataaagttac tattatccag gaccaatatt tattccaagg aacttaaaaa attatactat
     4981 tgtacagatg tctctcaagt tttcgcatca atttttaaaa ggacgagtaa cgcccaccat
     5041 aagtgttata cgtccggctt caatttcatc atcaaacgat gtcattatag gagtacccct
     5101 taaataaaat acagctacta cagaaacgtc aaacatagtt tttgaatcta cagagtagta
     5161 agcataacct aaagataaac tattcatcat attggtctga tcaccatcta ctgtctcatt
     5221 tttataagtg gtgctattaa caccgattaa tgctccatag ccgatagttg atacaataaa
     5281 agatgtacca aaaagatgtt tacgtaaatt taaaccggcg gttattccag ccaggttatc
     5341 cctataaaaa aaatcatccg agtattccga ttcagtgttt gttaaaggat attctccata
     5401 cgaaagattc atcactagtc ttgggcctac aaatacacct accgtacggg atatcgaata
     5461 cagatacttt gcatcaagct cattttgagt ccagtaaaca tcataactac cactcacagg
     5521 agcctcacta tcgctgctca ccggaatctc cgacatattt gcagtaattt gtgctccaaa
     5581 aaaaagggaa tttttagccg accgtacatt tagtagtgac gcggaaagag acactgctat
     5641 agccggacca tcccctccat ccgaacttac ataatcataa tcacgaccca ttattttatc
     5701 gccgccgccc gcaattcctc cgccagcccg aagagtataa taagtctcgg aatatagact
     5761 gcctgtaatc atgagggccg ctaaagtcat acttaaaaac atagatgttt ttctcataac
     5821 tcttcccccg gcacgcatat aaaacggaga agccgttagt acagcttctc cgatataata
     5881 cgaaataaat actctaaagt caattcaaag tattatgaac tcttaataat tgacttttta
     5941 aatataccac atattctcac caaaaagaga catcttgcaa gtaagaaaag aaatttgcga
     6001 aatttttatt tttcgaaatt cccagttata aaaccagcgc caacacagcc agaatatcaa
     6061 tcactatcaa ggttggcgta acttctttat gttttccggt taaaagcttt attacagtcc
     6121 agcttaaaaa tccccaaact ataccctggg taattgaata ggtaagagga atcagaacaa
     6181 gcgccaaaaa agccggaatg gcatcatcaa atttggtcca gttaataaga gaaaccggct
     6241 tcatcataaa aactccgaca agtacaagag ctgtggcagt agcaaccccc ggaactacag
     6301 agagcaatgg agaaagaaac ataaacggaa gaaacaaaag accagtaata actgcggtta
     6361 aaccggttcg accgccttcc tcgacaccag ttgccgattc tatatagctt gttcctgccg
     6421 acgtacccaa aagtcccgaa acggtagtag aagcagcatc cacaataaga cactctttaa
     6481 cgtgaagagg ctctccttcc ttgtcaagaa gatcggctgc ctcggcaact ccaataaatg
     6541 tcgaaatact gtcaaacata tcggtgaata aaaatgcgaa aattacaggc cacattgcaa
     6601 gtttaattga acctataaaa tcaagtttaa aaagcagtga aaaatcagga gaagcaaaaa
     6661 atccattata ttgaaccagc atggaacttt gtaccggaga gatccatgaa ccatcgccat
     6721 aaaaacggcc tataggtatg caaagaaggg tggtagccgc aataccgatt ataagtgcgc
     6781 ccttgattct tttaacaacc agaactgctg tcaaaaacag accagccgca aaagttactg
     6841 taacagcatt aagcccttta aacgaaatca gggtagccgg attgtcaaca atgaatccac
     6901 cgtttattaa accgataaat gttataaaca gaccaatacc agcggccaca gcaaaacgaa
     6961 gctgttttgg aattgcctta actatatgag ttcttacatt aaaaactgat aagataataa
     7021 atacgatacc cgaccagaaa accgccccca atgcagtctc ccaaggcacc ttcatgccaa
     7081 ttacaacgcc gtatgtaaaa aaagcgttta gccccatgcc tggagcaatt gcaattggtg
     7141 acttcgcata aagacccatg gcaatactgc tgaaagcaga aaccagcact gtagcggtta
     7201 aaaccgcacc gaaaggcaat ccggcctctt ttaaaattgc cgggttaacc actatgatat
     7261 acatcatagc aagaaatgtt gtgacaccgc caataatttc gtttttaaca gacgaattat
     7321 ttgcagaaag cttgaacagt ttttccatta cacccctctc taataatttg aaaaaatttt
     7381 taaacaataa cgctatgtga tcattattta taataattat tctgagataa agtgttttta
     7441 cgtatataaa gagaaaagtt aaaaaatttt acataaaaaa tgcttttcag aatagcataa
     7501 aaagcaaaat agtgataatc agattatggt aaagttttat tgaaaagacc tgttttttgt
     7561 gcttatatac atggaaaaga agcggtaatc atgttatcag cgatatctgc aataactctt
     7621 ttttaacaaa atggctattt ttgtctctta acatacggct aaatttaatc ttaacaatgg
     7681 agttcacact taagatgaat ctttatccaa gcatgccacc tgtaataaaa cgaaagcgct
     7741 ctctcttcac gagcctgttt tatacattat ccctgctttt catttttgga acagccattt
     7801 ttatactgta cacaaatagc agagcagcaa aatcagcaat aaaaactgaa tttaacgata
     7861 ttaatggcaa gattatcagc aaatttacac ttggagaaga tatttctcca aaacgctatc
     7921 ttgttataca acagccaaac aatatgataa caatgttaac cgtcgaaacc gaaatatata
     7981 acaacttaca attaaactca ccggtcatct attcctattc atcctctaat ggctctttaa
     8041 aactaaaaaa aatacgccag aatgattcta actgatcatt tcttgcccta aagtaaccaa
     8101 ttataatgtc cgaaaataga aaagagtgct taataatctt tcagcgttca cttaaaagaa
     8161 acaccgtatc tcccgcgcca agagcctctg aacgaaacaa tacggcagaa aaatggaggc
     8221 attatgtccg ataacaataa atccaaaaca gatgaaaaac taaaagaacg tatagctaat
     8281 ctatcacaaa atctggaaac tgatgaactt caatctattg tgcgccagat ggagatacta
     8341 atacataatc gcgaagttgt tcgcgaattt caagaacgca aagccgcacg aaaacttgct
     8401 cgtgataact cgaccgaaga agaaaacctg gatgtaaaaa taatagagag cgaagacggg
     8461 aagagctttc gcctattcgt tggaaacttc ccaaaaatgt ttatgttaga cgagatgaga
     8521 aggatggtga agttatgcca ctcatcaaaa acccccgaag agggggcaaa aaaacttttt
     8581 gtatggatca acaataacag aaaagacttc tttatggaca tacagaccaa taacaaagaa
     8641 caggttttct tgaatatccg taactgcatt gttgaacgat atacggtaaa ataggaagtt
     8701 atatattctt gccggcaaaa aattttgaat acttcttatc tgttcccaaa atatggttaa
     8761 caagccattt cttcaggaac gcaagaagct ccaaagaaaa agcctgttct cctgattcca
     8821 acctatcttt atactttttt acatcggcaa caagctgttc atgagctttt ttatggacat
     8881 caaagtccgg atacgcatag gcatgcatct ccttctcttc atgataaaaa tgcttactcg
     8941 tgtattctaa aagcctgtca agtatatatg tcatctcttt aataccaaac ccagaggtta
     9001 gaccttggtg cagttcgtta ataattgcaa ttaaagactg atgttcctta tcaaagatat
     9061 caacagaaac actatattca tcactccaat caataaaagc cataataatc ctcctaaata
     9121 tcacttactg tataatatta gcgcaaactc tatatctatc aacaagtttt tgatcatatt
     9181 tagcaaaagg aacttcgaaa aattactaca cctataactg taagagatgt taacgacgat
     9241 atcatttttt acacacatca taaatttaat ctaaaaaccc acccatgctg attaaaataa
     9301 taggacgtga aaattaaatc taatcaatta tacgacataa gcaaattttc cgcaccagac
     9361 ataaattact ttacaaactg ctgcgtatta tgttacattc gattggtttt cattattaat
     9421 tagcatttaa cagcactaaa aataattatt ccaaaaaatg gctctaaggc ttaaccggat
     9481 atttatgaaa atttctgata ttatacaaaa agacagcgac agggtttttg tcaccgattt
     9541 tggatgtttt atcatcttta ccggcaacac tcttgatgat tcacagccct ttatccgtat
     9601 aggcaactgg atggatatgc ctgtcgaact gataccgctt gtcgagaata taataattac
     9661 agacgatatg cctggcaatc cggctcagga acagttcaac atcgatataa acacacaatc
     9721 atctaaccgt tacataggta gccactatac tctcaaaaga tttcttgatt tccaaaagat
     9781 attcggtctt gatcttaaaa acgtgacaat tgtagacgtc gagaaggata ttcccgaaat
     9841 aagctccaga tctttatctg accgcgagtc ttttattgga atcttttatc gtgacggaaa
     9901 cttcaaaata tcacacaaca aaaaggatct ttttgaccta caaaaactgc ttgaaaagac
     9961 atacaacaca aatcgtgttc atgatgattt gtacgaaaac acaaaatcaa atcgagcaca
    10021 tttcggctct ggttttacaa tcgcggattc actccccctc ttttttgatc agggaagtta
    10081 ttttttatat atgctgcccc aaaactatca acaggttttt gaccaatatg gaataaaccc
    10141 acgcaaaata tcggctattg cgcatccttc ggccaattac ttgaacctga ctgccttctt
    10201 gcgctggatt aatcatagtt cttcgaaact cacaatttta tctgacgaca agcctcaaat
    10261 gaatatcata aaaaaacttt tttccagagc aaatctcaca tgccgttctt ttagcatgtc
    10321 aaagaccgaa gtaacccccg gcatgatcgt caacgaaatt aacggatctg gcaatcttga
    10381 agttactttt aactcggaaa caagtcaaac tgtaaacttt ttatatttta ataactctac
    10441 ggatatagac aaaataataa agcagacagc agattttttt gttattccct attctgtttt
    10501 tgaatgctgc aacctggttc ttaaatcaac aaagaccccc tattttatac ttgaagatag
    10561 caatcccaat tttagcaagt tatcgtcttt gactcattcc gctcttttta aagatgttaa
    10621 ttaccaagta atcaagtatg aatcatcaaa cgattgtctt gattttttta attccataat
    10681 ttcaaacgat atcgttaatg ctttaatgaa tccggataat ccggataaca acaaaatgat
    10741 aaacgatttt tttgaaaatg aatcttctcc gtttgatcgt acaaactgta tatcgctgat
    10801 aaaatacctg attcatattt caacaaaccg taaaatatca tcgtatctta aaaagcttat
    10861 tcaaaactac gcagttcaaa acagcgtgca taaccttaaa gcgatggata ttgctttttc
    10921 ttccaaaata atcgtagccg acgagggagt tttcacagta tataccccca tatcacgttc
    10981 aaaccgccct aaaaactata ttccaatggt acctgttcag attcctactg acgaatcgag
    11041 ctataagaac agcatcgaat acagggacta tgcggcaaaa atagaaaatg acagaaaaag
    11101 acttcatgca ttggttcaac tttaccttgg aaacggcaaa accaagccag taagatctct
    11161 taaaaaagca attaacgaaa gaaaagagat ttataagcag aatattctat cacgtgaaga
    11221 tattaacaaa cttcatatta atcagatttt tgatgacaac aatcaaaccg atgtttctaa
    11281 aactaaagga attaagcctc tatttacaaa aattatacga agaatcaaaa cagctgtttt
    11341 taataatcaa aggtataata aatacgttca aaacaagaat gagtacgccg cccacaaaga
    11401 acttaaaggg catcactcat ctctaattca gcggattata caccccttct ggtacagttc
    11461 ccccataatt ttcttcagat ctctaaacag gatattaaca gcatttttaa ttatagtgct
    11521 tgttattgtt cttttttctc tatattggaa agcttatcag aaaaaaccat ttattgatac
    11581 cattgccgaa cttattagtc gtggttcaaa aactacagca tcagagaaca ttacgtcatc
    11641 ccaaaacgat gaaagttttg acgcattggc aaataacaac agtaccgatg acccggaaaa
    11701 aaacggcaca ttaattacag attcagatca taatacagaa gccacaaatt tagaagacaa
    11761 cactgaaatc aaaccggtca ctcctccaac cgaaataagt gatcttgagg agcgtcttga
    11821 aggtaatgac aacgaagaaa ttattgaaaa atttaaaata aaaattacgg ataccgagat
    11881 actctattat cttaatgaga tcgcagttat taatggttat cgccctctag gaccagatga
    11941 agttaagggt aaaaaccctc attggatttt tccaaattct atctttacac ttcctgatgg
    12001 tgaacgtttt atcgttagag atggtgattc catgtggaaa attgccgaac taaaacttac
    12061 aaagatgtcg atagagtttc acaaagctgt ggaaagcatt aaatcgttca ccggcacgaa
    12121 acgtcctgac gaactttttg aaaaagccaa taaacatagc tttggtgatg cccagaaaaa
    12181 tattcttaaa ctattggaag atcgctttaa gtaatgaata cttataattt tatcagaaaa
    12241 cagctttgaa aattaccaca actgtttgcg tttaaccgca ggtacaaatt attttgattt
    12301 tagtaatttt gaaacaccca tttttaaagc gaatttagat taccagcaat ggttggcaat
    12361 ggactcgaca gataaaaaca actattatat tgaaccagta gagattgaaa tatatctcaa
    12421 gaaagccggt acggttaaaa caataatcaa ggatctctat atagaacttg ttgacgaaga
    12481 ccccttgcca gaaaatgcca ttaaaatatt tgactatttt cgagaaaaag accaacctat
    12541 tgatttaata gagttacaaa accgcttccc cgaaatgata cttcccgtta ttgaatctta
    12601 ttacagattt attgatttgt atgaaaaact cagtatgcat ttccgttcag ctctctctgg
    12661 tagcattgac tcattacgac aggctctcta catgaccgag cttcttatga aatacgaacc
    12721 cactctatgt tctcttaaac taattggtga tttttcgacc tataacctca attacctgat
    12781 acgaaaactg aatcaaaaca atgaagaatt tttactagaa gacagcacgg tggagtatct
    12841 tataaaaagg cacagatcta aacacgaaaa tgatgaagat ggatccaata aagagtttga
    12901 aaaacttgta gagttgtggc aatataacct gaaagagaaa cccatactat agaattattt
    12961 gccaccatta ataatgtaat aagctacatc tcccagtatt acaatccata ctaaaacgga
    13021 aaataaaact agggcatacc aaccggcctt ataaacaagc tttttatttt gcctttttaa
    13081 actcattaat catctctacc atcatagcaa catgttgtgc caatagatct atagcttgcg
    13141 cgggatcagt ctgtgactct atcaaagcca tataaagatc cgaaaccgac tgccgaacaa
    13201 gctggtcaag ctctcctact ccacggccac caccacctgc ttgctgttga aaagcgtctc
    13261 ttcctagcgc cttattaagc attcgtttat gcaaatcgtc cattaaatct ccccgcaaaa
    13321 caatcaggca tcaggatcat ccgataacac cggatctttc gcgtcttttt tttcaccacc
    13381 ttctttggtg atatcatcta ctactctaac acggtcagcg aaactgtcaa ggacagattc
    13441 tcgggctaaa cgtgaataat tatctagatg ctggatataa agcttatcat aatccagttt
    13501 atatttaacc ttgtcttcgc gggcacgcat aacctcaatc gtagcggcaa tcagcttaaa
    13561 attgaagtca tttacatact tgacgataac ttcattcgca agataaaatt caccgtttcg
    13621 aacataattg gcgactctct gttcctgacg aaaccgcaag cggcgttcga tcttgttttc
    13681 ataaagataa tctacagctt ccggattgcc tggagcaacc gaaacaccac ctccggccgg
    13741 atcactgtcc agttcggtgc cacttgcaga tcccccggtt ctatcgggaa tttttccagt
    13801 ctcctcataa cgctttaatt ctgcttcaag cttaggaccg tactcatttt cataatcttc
    13861 ataatcacga ttaatctcgg caacaagggc tttatcgtct ttggacaaga acctgttgta
    13921 aaaaggatta ttgttttcac gttcaatttc aaaaagatgt ttataaatca gttttttggt
    13981 ctcgatatcc tgactttcga acagcgccaa caaggcataa cgcatggaac gacgagagta
    14041 ttttatagct tctataaacg catatatttt atgggacctt aaatgcggcc ttgaagcgtt
    14101 accaaccatc tgcacattgc gtgctagagc acggtcacga tatccaaggg ttaaatattg
    14161 tctagcggca ctgtttttgg atttaattat tcccggagca agacgatcca gtacgatctt
    14221 ggactcatca agataatatt catttaaaac cttgttgtac aaatgaacat tcgcgtcttg
    14281 tgatttatat acgtccttaa acgcacgatg ataatcagac tgcagaaaag ccacttcgcc
    14341 attaaaatgg ctctgataaa cattataaaa tgtatccagc ttttccgaat ccttatcaga
    14401 aaaattactt aaacatatat ttaaaaattc aacaaacaat ttattttctt ttataatgcg
    14461 ctctgttgtt gtctgatccg tataaccgaa acatggaaca agataaaaga gaataatagc
    14521 tgacaatagc tttttcttca ctgcactccc tccggggatg ttttaaccga gtatataacg
    14581 gtgaattatg taattttctc aattattaaa tcatcactct aatatatcgg taaaaaaggt
    14641 ctatttctaa aaaagaatta caacaatcat ttttatcctt tttcagataa aaatattccg
    14701 actccttgtc ggctatataa gactctccat ctcaagaaca agctccttaa aacgcgacaa
    14761 ggtactgttt acggcatctg gtctggacat atctactccg gctactttaa gttcttcaat
    14821 cggatacatg cttcccccaa gagttaaaaa ttgacggtaa ttatcacaag ccccttgctt
    14881 tccttgcaat actcccgacg caagcgaaac agcagctgat atacccgtag cgtacttata
    14941 tacataaaat gccgagtaaa aatggggaat acgtaggcat tccagctcta gagtgtcatc
    15001 aataactatg gagtcaccga agtattcttc tagaagcccc cgatagatct cacgaaattt
    15061 atcaattgtt aaaggattgc ccttctcaac ttcagcatga gaaagatgtt caaattcagc
    15121 aaacattgtt tgccggaaaa gcgttccgcg aatattatca atttccctat ttaagatata
    15181 agccttcatt tttttattat ccgcgtattt ttttaaaaga taatcagata aaagcgtttc
    15241 attgaatgta gaagcaacct cggcaacgaa aatagtataa tcgctgtaaa catagggctg
    15301 tttttgcctt gaaaaatagc tgtgcattga atggccggcc tcatgcatca aggtatatag
    15361 actattgata ttggttttat cgtaattcat aagtatgtag ggatcagagt catagcatcc
    15421 agaagaataa gccccacttc ttttgccacg gttttcatat ctatcgaccc atcctccaag
    15481 aagcccttct ttcattattg aggcataatc ggcacctaaa ggagaaacgg ctgcgacaca
    15541 tgtctcgact gcttctttat agcccataca aaaatcgaca tcatttatta ttggtacata
    15601 agtatcataa aaatgcagtt ctttaacatt tagagctttt tttcgcaaag acatataacg
    15661 ttttattaca tcacctgact tttttacaga ttcaataaga ttatcataca ctgctattgg
    15721 tacattatcc gtgaatagag cggcttctcg acatgatgaa tactttcgca aacgcgcttt
    15781 aagccagtct tttttaacag aaaacgacaa tgaagacgcc aatgtatatt tatgattatc
    15841 ataagcatca taatactgat gaaaagcgtt cttacgtaca gcagcatcgc agtcatgtaa
    15901 taggctaata aaattaccat gagacagctc gatattttca cccttgccgt ttacaatatc
    15961 tccaaagcga aggtcggcat tgtccagctg agagaatatt ttacctgcag tactaaaaac
    16021 ctcgcctgac atggccagaa tctcttcaga ctcttttggc aatgtatgag gtttaaatct
    16081 tataattttt tcgagataaa aacggtactg agtcaattcc tgggattcaa gatatttttc
    16141 aagaatctca tcatcgagtg actgaatttc cggcacaata aaactggatt tttcggatat
    16201 ttttgtaaat agcgttacag cacgctgata aagtgtttct ccggaatcat tggttttatc
    16261 ctgatcattt aaaagatgcg cataaagata caccttgtcg attttacggc tgacttcaag
    16321 gtcaaactct atcaattcaa gacatttatt cacagaattt ccggccgttc cctttaatga
    16381 atcatatttt tcgatcatct tctcaatttc agaataatcc ttttgccatt gttcaataga
    16441 ctcataaact ttttttatgt tccatttatc cgattcctct atttgacttc tatttggtat
    16501 tgctttagag ttcatttggc tctccatttt tttatattct aatcgaaaag tgcaggaatt
    16561 actatctgct ctcttttcct tttttcagac agagtatcta cagtgaaatg gttttgtgaa
    16621 atattaaaaa acagtgcacc attactacca gtacgatata aatcaatttc ccgaacagag
    16681 agattttgca taaacatctt actaaatttt gacataccat ttattaaagc cacttgatca
    16741 gttttagtac cattaataat atcaatggca aaatcatgct cctgttcaga attaatttcg
    16801 ggaataacta tcagtgaaaa accggaaccg tcatctatta aattgatatc aaaacccttt
    16861 aacgaattca acaaaaggac tctttgtccg aatatcgaca gaatcatata ctcttcttcg
    16921 aaaccttcag acccaaaaat tattcgtgat gaaggtgtaa ggttgtaaaa agatacaaat
    16981 gctttttcta tttccttagg cagccctgca aaactgtttg tctcattcgc gagactttta
    17041 ggtaaaagca gcgtagaagg cttaaaacct tgctttttta acgtagcgat atcagaaatt
    17101 atttgcgagt taacctgaga agtaaacagg gtcgatatac ttgtagattg cattgaacga
    17161 acacccgagc taaccgaatc tgtatttatt gactcggctg gtgagataat caatgctgaa
    17221 tcaccggtat aggttataag agacaccacc ggactttctt gggtgtgata gacaatcatt
    17281 tgcatttcac gctcaacagc agtaatactg attaccaaag gagtcaaaag aatcactaaa
    17341 gctgctaaaa atggcaatgc tcttaataaa aaggagttta aatggttatc gatcttataa
    17401 aaaacggctt taaaatatgg taacagttta aacagataaa gacataacaa tagaataata
    17461 aaataatata ttatccaaga aaaatctggc tttggtaaag ttggtgattt taaaaaatca
    17521 ctccctgcat gagtgattat caatagaaat ctggaaagta gccagctagc accacctaaa
    17581 ggcaaagcta tccactcacc aaaaaatgga ataagacctg tcaaaccggt tatggtacct
    17641 aaaaaaagta tcatccctat tacgggtact gcagcccaaa gcaaaaccag tgaacttgta
    17701 gggaaaagcc ctgtgtccca taccgataga ggtatcaaaa caccagcctg atatgataat
    17761 aaaaatgcga cgatagcctt tatcggtact ggaaaagaga taaacgacaa gttccagaat
    17821 agaggaaagc ggttatttaa caaacggcca atacgtacaa ttccccatgc tagaccagca
    17881 attactatca agtattcagg gcggaaaaag tattgtgcac caatattgat taaataaacc
    17941 aaaagcagat aataaataaa aaatattatt aatgtaaacc catgcaacat gttcaaccag
    18001 cgtaaaaccg gaggagttaa cccgataaga accaaaatgg aaccaaatgt cagcataaac
    18061 tgaaaagtaa agagtgaaaa agggttataa agacatatca cgataactgt catcgatagt
    18121 ccaataattg cggactttcg aatattgtaa tggagatatg aaaaaacagc tattgctatt
    18181 gctgttacgg tcaaagcctg aacagctgct ggccgcgctc cacttaatag tgtaaaaagc
    18241 agtattaacc caataattgc tggtgccaaa tgacgagtct tgaatccaat tccccaaaaa
    18301 agggtgtaaa gcattaatga aagaataact acgtgaagac ccgaattttc aataaatact
    18361 gtaactcccg attttttaaa tatatctgta attttttctt cttcatattc gatcatatca
    18421 agtgagcgac gtgtcccaag aattacagat gttacaatgc gactctcggg cgaaggtact
    18481 gtacgcctgt atgtttgtat gaaatattct ttgagtttca aagcaatatc ttttagggaa
    18541 aaaaagtccg ctcttttggc aaaacccaca tcacgactgc cacatgacag aagtactccg
    18601 atattgtttt tttgcatata attaatataa tcgaactcgc tcaggttaag gggcgcggca
    18661 ggactaattt tttgcgcatt tatatccaga tatgttccag caatttcatt atctgataga
    18721 gtttcaaaaa acgaggcctt ggttgtatcg ttcttaagaa ttttcacaag aacttttata
    18781 ttgtctgtat cgacccattt attagaggta aaatcataaa ctttttttac tgaaagcaaa
    18841 agctcggttt ctacattatt gcgaagaata ggttccccag taacagttcc ataaatcctt
    18901 attgtgcctt ctgggttact aatataagca tcaagatcaa tatttactgg ctgcattgct
    18961 atatgcattc gcatataacc cgaggccata ccaaaaacaa caaaacccag tattgaaaaa
    19021 attaaagaaa attctttcca cttaagttca caataaagta taaacataca aataggtaaa
    19081 gagatacaaa aagctaaaag agcatatatt attggaacgg aagtaaacgt ataaagagca
    19141 atacccgcaa gaagtcctcc tgctgttaaa aaaaagtttt tataaagcat tattgatctc
    19201 cgggtatagg ggctgtatta taaaaatctc tatttgtctc ttcaatttct tcaaagcggt
    19261 attgctcatt tgttacaagc acgctcaagc ctaatattaa acatataata aaaaaaagag
    19321 ctactttcat aattctttgt ttatgcagga catctatctg ttctgataca tcttttaaga
    19381 tcaccttgca acccatttta tctctaatga gcagcgttaa gtacgataca aaacgcttaa
    19441 aattatgaga actaacatta cccagaagct gtcgaggata aagagagtta tcacgagccc
    19501 ggcacgttgg tgaagctgtt tcagatccgt caccatctat acctatacgg ctcattaaca
    19561 agaaaggatt taaggcaaca gacctgtatt ttaaccatct gaaaaatata agcccggatc
    19621 caaaacaata gagcaaatat gacgcttgtg gaaatttacg cctataatag aaagcggctt
    19681 caccaacagt taaagccggc cttttccgtc caggttttac cgatttcgct attaatatcg
    19741 aaaaatcttt ttcaaacaga tagttttcat tatcatatgg gacagaacat cgaagttcga
    19801 aaacggactc aagaagttta tcctcaattg aggcatgata atcgtttaat ttctgaatat
    19861 cggtaaacca tttttttcct cttattgaac gaataaggga atccgaagaa acggactttg
    19921 ctactgtttc aataatttcg attatagaaa ttggactacc cagcaactcc tctatcatat
    19981 caaattcttt agacgttcga tcgccatctg agcgcaaaat ttttaacagt gtttgtgtgc
    20041 ctttttttaa aggaattgtc tctttagcaa atgattccat aactgactcg gtactcagta
    20101 agccgcgttt atggttaaat acattagttt tttttattag tcctttatgg taaagatttt
    20161 ttatcaattc atcttcaccc tgtcgacact cttgaacatt gtattcttgc aatactacat
    20221 tcttctcttt caaataagcc agatcatcct caaaggaggg tataacgtgc ggcggaatat
    20281 cgttgatgta aagagatgca ggtgaaaatt tttcaagtga tcgcaaacct tcgtaatact
    20341 gaacaaatcc ataaacataa tctttgccat acttacagtt gttttttttg tgttttagtt
    20401 tatacgctgt tagtgcagct gcaaaagctg aatcagcact gcatcctgat tcataacgaa
    20461 cctgtatcga atttacaagc tcataaatac tactgaattc tatatctgtt gaatattttt
    20521 ttcgtatttc atcttcatga agcataactc cttcagtgtc taatagggaa agtttcatta
    20581 tttccatttt taaaagagtc cgatcaagtt ctcgtaatga tctttgctca agcagttttg
    20641 tccgatattt gcgttcaaag taactaaatg gcaggccaaa atctccaaaa aaatagtgca
    20701 gctcttcttc attatattca taaaatgcac cttttggtgc agtaacaggc gaaaggctgg
    20761 cttttaacat ctttgcaatt aaaagctttt gatacgaagt aatgagcttc gactccctgg
    20821 gaaaggagag aagtcgacga attatgagac gatctgacga tttaaaataa tagataccac
    20881 ctgaataaca cacctcaatt tggggcaaat caatcaattt gtccagagtt tcatggaaaa
    20941 gaagaccact accgtgaacc actattacag cacgcttata cgacataatt tatcctgttt
    21001 attatattaa caaattgact cttttacaat aaaaccaaaa agccccctct tttctacaaa
    21061 taactctttt tatagtctac aaatttttaa taaaagagca ttcaaaaaaa tttgatattt
    21121 attttggata tagtacattt atcacattaa caactactga aagtatttat cagggggata
    21181 ccgtgaagga atatagtgaa aaacaaattt taaacgcgaa agatcccgcg caatttattg
    21241 acagatgtgt aaattcgaat gtttcaaggg gtaaaaaaat agttttgggc cgaaaatggc
    21301 gcgaaaagac aggttttaca atggaagaca tcgaatatgc aagaaatagg cacccctact
    21361 ggaaagagaa aaagatggcc ggttggcgtg aaagaaatga aaaaagatgg aatgcatata
    21421 actttacgtc aaaagagtca cttgagtctg actattggac taaaaataat tttgccaatt
    21481 tgcgagaata tatcaaaaaa gacaaaaaaa attcaaaagg ggtttacaac gcaaaagata
    21541 gggaacttgc tgaatttttt agaacatcta ttccatcaat acaacacatg cgaagaaaat
    21601 ccaatatggt tacaaaaatc atggggaaaa gaaaaataaa agacacagta aaaaatagac
    21661 tggattttat gcagttagga gaaaccactt tgagagaaat gcttaacaaa aagtagaaaa
    21721 gtagatcaat tattcaaaag cccaagcata aactcgggct tttatataat gctcctattc
    21781 ttcatcatcc agatacattt tatctatcac ctctttgtat ttttcctgaa taacattccg
    21841 ctttattttt aatgatgcgg tcaactcgcc tccgtcaatc gtccactcgc ctgacattaa
    21901 tgtaaacctt ctaatcagtt ccacacgtga cattgaagca gtaagagaat ctatctcttc
    21961 tctcatcaaa gcgattgcct caggctgttt aacaatatcc tttttattca ttccagaacc
    22021 aacacctctc gcagccaaac gagccgataa ttcctcaaaa tcaggtagaa tcagagcact
    22081 aagaaatttt ttattatctc cgataatagc gatctgttca ataagccgcg aggttctaag
    22141 tatctgttca attggctgag gtgaaatatt ttttccacct gcagttacaa taatatcctt
    22201 aattcgacca gtaatataca gaaaaccctc ttcgtcaaca atgcctatgt ctccagtttt
    22261 aaaataacca tcttttgtca taacctcttt tgttgcctta gcatttcggt agtaaccctt
    22321 cattacttga ggccccttga tgagaatttc cccttcatca ctgatcttga tttttgtatt
    22381 tttaaaaggt ttacctaccg accccaactt catgtcatca ggcagaacaa ggttagtaac
    22441 aggagaagtt tcggtaagac caaagccctc aagcacagtc acccccattc caacaaaaaa
    22501 tgtcatatca ctatacgcta tggggcctcc tccagaaatt gcaaatttca gattatccat
    22561 acccaaagct actctaagtt ttgacaatac cagtttctct gcaatcgcat gaggtttttt
    22621 aagataaaat ggcaacggtt tctttttgca gtgataaggt actgcctttt gccccatcgc
    22681 aacggaaaac ttgaaaacag cctttttaac aggagaggct tttgtaacct tgccaacaat
    22741 tgctgaatgc attttttcgt atattctggg aacgctcaca agaatagtcg gacgagttgc
    22801 aacaagatct tgctgtatgg tagtaatact ctctgcaaaa tagacctttt tacaagtggt
    22861 catgggataa tagtaaccag ccgttctttc aagaacatgc gaaagtggca agaaagataa
    22921 gaaggcatct gtttcatcca aatatttgcc catagcttct tctatctgac cggtatttga
    22981 taaaaaatta tgttgagtta actcgacccc cttaggctct ccggttgttc cggaagtgaa
    23041 aataagagtg gcggtaaact cacgctttac ttttttaaca gctgcatcga cacgtttata
    23101 atcagccggt ttaagctttt tctctatcag gtctccataa tacttaatat ttttcttttt
    23161 tgtttcgatt ttatcgaaca taatatattc ttttaacagg ggcagctttc ttgacataag
    23221 ttgaagtgta ttcaaacgtt cttcagtatc cacaaaacat acttgtgcac cacttttatg
    23281 caaaatcagt tcaaccgtac caactgcact tacagcatgc aaaggaacag taatagctcc
    23341 gagtgataat acagcaagat cgtataccca ccattcaggg cgattttcgg aataaacagc
    23401 aaccttggtt ccttttgaca caccacgaga agccaaatat gatgcgatga catagacttc
    23461 ttctttcacc gtatcccatt ttatatcttt atactgtccc tttaccttaa cagacaacaa
    23521 gggtttatcc ggattttttt ccgcgttaag gaaaaagatc tgtggcaggg attgatagtc
    23581 atcaaaatac atgctgtgtt ctcctttgtt ttatttttac tgaatataaa taagttgttt
    23641 gtaaagatag gctgctaaac agacattatt tagcaagctt ttagattaca tttatttact
    23701 agtctgtttt tttcaataac tttatttata acgtaaccca acaattattg cagataacgt
    23761 taaattttta tagaagccca taatcttttt gtttacaaaa gttgtgtcac ttgtcatcct
    23821 gataatcaat taacttctac aaatttataa tgatgctttt ttgagaacaa tggttaaaat
    23881 gtataataca gtataataat taaaacatat tggaaattac tgatggaaag aagatatcta
    23941 aaatgtcgcg aacttagcca aaaaagagcc atagaaataa ttacaatgat taacagtatc
    24001 tggcctccca aagatatatt atcggaaata cagtattctg aacgctatat tgcaaaaaat
    24061 gaaaataacg atctgatatt atatcttgat agcggtaaaa tattcgcaca cgcaatgtca
    24121 tttatcaggg agataaaaac tgattatggt ttgatgaaga ttttagcaat taaaggtata
    24181 gctgtaaaac ctgaatatag agataaaaac tttggcaaaa aaattgttct ggacgctttt
    24241 agtagcctag atacaaaaaa tttgaactta tctctttttc aaactggagt tccctctttt
    24301 tacaaaaaac ttggagctcg aaccgtaaaa aacagattta ttaattctct taataacgaa
    24361 gatcctcaat ctaatccctg gtgggataaa gaagtcatga tttatcctgg ttcagccgaa
    24421 tttccagatg gcgtaataga tcttcttgga tcgggatatt agatagacca tgttcttttc
    24481 acaacctgaa ataattgcta ttatcgtttt gatcactgct cttcttgtat taatctttat
    24541 cgctggacgt aaaaatggca gttcatccaa cgaaaaacgg tggcagcacc aattgaaatt
    24601 tattaaaaag gatattgccg attcttccag gtctgtaatt agaggacagg tgtcggagca
    24661 gatagcccct tttctacccg gttttccatt tagcccttct ggatgcagat tcattggcaa
    24721 gccggttgat tttattgttt ttaacgaact cgaagatccg gataagtgct caattattct
    24781 tgtcgaggtt aagaccggtt cgtcttctct taaccgtaat gaacgtgccg tcaaatcggc
    24841 tgtcaagagt ggccgcgttc actactacga atatcgtttt aacggtgctg ataactgaaa
    24901 acgtggctag caatccacaa aaattaaaaa ttttcttcta aagcgacata atagtaaaaa
    24961 taattttaaa aaaaatcaaa aaaaacagtc tctatatata taaaatataa tggaggctat
    25021 aaatgaaaga agataaccca aatattagaa cttgtgtaag cgttcaagtt gatctgttag
    25081 acaagattat tgatttttca gacaccaaca atataccagt atccgacata atcaatgtac
    25141 tcttgcaaaa acaagtagaa aagctggcta gcaatccgca aaaccgcgag ggagccatga
    25201 aataccagga tcgcggacta caatataagc cagtgcatct aaaaataagc tatatacaat
    25261 acagaagatt gagagatatt atgtttttat tccgaatcac tctctcttat atgttatttc
    25321 ttgccatgca atacttcgac acttacctaa acgagttcaa aaacaatata aacgacaagt
    25381 ttcctcccgt atgtcattgc tcaattctca ctttttttga ttctttacct gtctacgctc
    25441 attattgggg aatcccacca gctgaacata caatttcaat accggctgga tagcaatccg
    25501 ggcaaaatga aaatggcgcc ccagaaggag cgccatatat aaaacaaggg gcaatatcta
    25561 aattacatca taccaggcat tccgccgccc attccaggca ttccgccacc cattggcata
    25621 ttatcattct cttcttttgc atctgttatt aaaacttcgg tagtcgaaag cattcctgaa
    25681 acagaagcag cattttgaag tgccgaacgc acaacttttg ctgggtcaat aactcctgcc
    25741 tcaatcatgt tttcccattt aagatctgca gcattaaagc caatacctgc tttttcactc
    25801 ttagcttttt caactatcac agaaccttca agaccagcat taaccgcgat ctgtcgcatt
    25861 ggagcttcca cagctttaaa gattatcatt gctccaagtt tttcatctcc atcaagtgac
    25921 tttgcagctt cctcaacagc tttttgtgta gaaagcagag ttaatccgcc accagggatg
    25981 attccctctt caacagcaga acgagtagct gatagagcat cctcgacacg agctttcttc
    26041 tctttaagct caacttcagt tgctgctcca acattgatta cagccacacc acccgcaagt
    26101 ttagcaagac gctctttcag tttttcagtg tcatattcag aactcgaatc ttcaatctgt
    26161 tttttaattt gagcaatacg atctttaacc tgagtatctt ttccgatacc ttcgataatt
    26221 gttgtattct ctttttcaac gataatcttt ttggcctgac caagatcttc aatagtagcg
    26281 ctgtccagtt tgcgtccgat ctcttcagaa ataacctgtc caccggttag tatagcgata
    26341 tcttccatca ttgcttttct tctgt
//
LOCUS       DAOTGZ010000011        26035 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_3064
            NODE_2165_length_26035_cov_5.947729, whole genome shotgun sequence.
ACCESSION   DAOTGZ010000011 DAOTGZ010000000
VERSION     DAOTGZ010000011.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868088
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 26035)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 26035)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:22:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,785
            CDSs (total)                      :: 2,751
            Genes (coding)                    :: 2,732
            CDSs (with protein)               :: 2,732
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 12 of 19
            Pseudo Genes (internal stop)      :: 5 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..26035
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2165_length_26035_cov_5.947729"
                     /isolate="AMR_MDS_3064"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(1..1257)
                     /locus_tag="P5123_01700"
     CDS             complement(1..1257)
                     /locus_tag="P5123_01700"
                     /inference="COORDINATES: protein motif:HMM:NF018884.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016791 - phosphatase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SpoIIE family protein phosphatase"
                     /protein_id="HRX15009.1"
                     /translation="MSDFSFDTLKKDYNNLRKLIEITKLINATLDIGKLLKAVMKAIK
                     DILHTEASSLLFYDELTDELVFKAVTGEKSDMLSEQYRFKTSQGIAGWCAANREALIA
                     NDVYSDPRFNPNFDKITGFKTNAIICAPLLFKGKLIGVIEGVNPIGRVMFTENDMKLF
                     TLFAEQAVMAVQNAIIFEKSIERTKMQHEISSAQKLFEPFLKPITIERDEINTTMICR
                     PAGEFCGDIFTSRSEKDHVAIMLADAGGYSLPGAITASYIAGKITAFGRVCRNCSEVI
                     RYFQSSRKDITVPKEASIFYAHLFTNRNCLEYTLSGNIRLILVQDRKIKLIKANSDIN
                     TGTIKRSKIEFSKGDTAIIFTEGVTRIKNKHGEQFGIKRLASLVQADLSSNEILDTII
                     QQTSLFSHKLRTFEDISMICIKRNQD"
     gene            complement(1254..2087)
                     /locus_tag="P5123_01705"
     CDS             complement(1254..2087)
                     /locus_tag="P5123_01705"
                     /inference="COORDINATES: protein motif:HMM:TIGR00696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="WecB/TagA/CpsF family glycosyltransferase"
                     /protein_id="HRX15010.1"
                     /translation="MSTEDVYYDSWKENRDLILEYNQVDLEDVTVSNIKGIGVDNVST
                     KQAIVKIMEMIEHGGVHHVISLNPYKLIRYRANIDLNIIYGKASLKFATGAGIRKLSR
                     IVRQPLKERIHFISFLMELIRLSEIKEYTIFIVGAKPEITEKAYSNILKSFPKIRIVG
                     RHGGYFNPQREQSVIEAIRKSEANIVLVGMGFPKEDRWISKLRMNLQNSVIIGIGGSL
                     DIISGENRKAPAFFMQRGLDWFYRIITKPWRYGRLLRIMFFFFTTFVGSLFISNRRYL
                     R"
     gene            2300..3139
                     /locus_tag="P5123_01710"
     CDS             2300..3139
                     /locus_tag="P5123_01710"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF019846.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HRX15011.1"
                     /translation="MNYTEKISRYIELNLKENLTIEREMCNKKQHDFVSKAIETYRPE
                     SVLKAGLGNGELLLKMAEVGIKVTVVEPSIEVINNFVKKNKDNVLIDNIQFINGDLNS
                     LPVDYYAFGMIVCMDYLDFQNTAAVFDEFKRAIDFEGVLVLGGIVLNNDDIDGIYDDL
                     YHIIMPMHNDFYLPTDLNTVLGLKHFSFIQNEVFNIPVELESYKSFAKKYGAEFSFSE
                     YNKADLFIEENRAQLAKMYEYDGNGAVEPYLLSLYKSEKPEKQEYEKEVEDYKRKTGR
                     AGA"
     gene            3271..4362
                     /gene="hydE"
                     /locus_tag="P5123_01715"
     CDS             3271..4362
                     /gene="hydE"
                     /locus_tag="P5123_01715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018249419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="[FeFe] hydrogenase H-cluster radical SAM
                     maturase HydE"
                     /protein_id="HRX15012.1"
                     /translation="MNYEIKYDTRDNIASLLSENNPKQVELIRKMAHDVLLENCGSGV
                     YYRGLIEFSNRCVSDCYYCGIRRSNADVNRFDLTMDEIVDSALWCAKNGYGSIVLQSG
                     ENSSRDFIDFVCRAVETIKKRSISEELPNGLGVTLCIGEQSEADYARLFAAGAHRYLL
                     RIETSNRELFKTIHPEAQSFDNRVECLRRLKRTGFQVGTGVMYGLPGQTYQMLADDIL
                     FFKDMDIDMLGMGPYIRHSSTPLKKNENQFIIEGDDLLRVSLLMIAACRLVLKDINIA
                     ATTALQTIDPTGREKGLSFGANVVMPQLTPVDYRKSYLLYDNKPCQEDTKEECKNCLS
                     ARVGSVGREVHYNEWGDSPHFKRKGESKN"
     gene            4359..5426
                     /gene="nadA"
                     /locus_tag="P5123_01720"
     CDS             4359..5426
                     /gene="nadA"
                     /locus_tag="P5123_01720"
                     /EC_number="2.5.1.72"
                     /inference="COORDINATES: protein motif:HMM:TIGR00550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008987 - quinolinate synthetase A
                     activity [Evidence IEA];
                     GO_process: GO:0019363 - pyridine nucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="quinolinate synthase"
                     /protein_id="HRX15013.1"
                     /translation="MKELIEQIKQYKKILGEDLVMPAHHYQRPEVVALSDFAGDSYKL
                     AVEASRHTSDFILFCGVRFMAEGASVLAADNQKIVVPELLAGCPMADMIDKSMAQKAY
                     DIISPLSNRRIAPVVYMNSYADVKSFCGEKDGAVCTSSNALKICSHFIEKGEAVFFFP
                     DYNLGINTADTLGLEDYEVVRIGRDLVIEERVDYKNVKMYLWDGFCHVHKVFTVDDVK
                     KAREQYSGIKIIVHPESDRAVVQASDMNGSTAFILDSIRDSEPGSVWGVGTEVNFVRR
                     IASDFGPNSTHNKTIVALRDSPCFNMEKTSLEKVAASLKSIVDYKNGEGELVTELRVQ
                     PEFKDGAKKALQTMMDIVQKA"
     gene            complement(5423..6727)
                     /locus_tag="P5123_01725"
     CDS             complement(5423..6727)
                     /locus_tag="P5123_01725"
                     /inference="COORDINATES: protein motif:HMM:NF015764.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HRX15014.1"
                     /translation="MKKLYPFSLLAIIMFLQLAIYGSYRPILSTYLTKVLNFSGTQAS
                     VIISTTVIAGIFSPLAASFVVDRLIKARHLLSILHFTAAFFSFFTAINTSFKSVLFSY
                     LFYMLLFAPTMGLVNAVTFKNLKSRREHFGSIRVFGTLGWMASGLILGQLYIKFFNGP
                     VSHIFFAASIMGIIISIICFFLPDIPLPPRSRKSIKDIIPASAFKTIARPELLSLTVV
                     FFLSSTMDQFYFFGTGPYLEHLGYSYGNNLAMQTLGQVLEIVSIPVLGFILGGFLINH
                     KGGSFYFKGIGFKKSFLLGLAFQVTRYTIFTLTGSKLGIVTALLCNGFIYTFFYATAT
                     IYVDTQCTEENRSGVHQIINMIFFGFSGLTGTITAGFLTDYYSKNGTTDYTMFWMVPT
                     GLSIIALILTVIFIKDVPVKVSTAKSISGTVDDTASGPHETV"
     gene            complement(6812..6885)
                     /locus_tag="P5123_01730"
     tRNA            complement(6812..6885)
                     /locus_tag="P5123_01730"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(6849..6851),aa:Pro,seq:cgg)
     gene            7088..7270
                     /locus_tag="P5123_01735"
     CDS             7088..7270
                     /locus_tag="P5123_01735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017873577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRX15015.1"
                     /translation="MLGFQDWGVTLAYVGTIVSAVICVVYGIVNWNRPREDEESEINE
                     ELEWEKNDPELKEGNI"
     gene            7270..8871
                     /locus_tag="P5123_01740"
     CDS             7270..8871
                     /locus_tag="P5123_01740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005998596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium:solute symporter family protein"
                     /protein_id="HRX15016.1"
                     /translation="MNMIFLGFVVILYLSVIAFLGYRGYKRTKSTSDYLLAGRKIHPM
                     IMAISYGATFISTSAIVGFGGAAAVFGMGVLWLTVLNIFVGIFIAFVFFGLRTRKMGH
                     NLDAHTFPELLGRRFDSQFLQTAGGLLIFLAMPLYAAAIILGGAQFMSTTLAIPYEVS
                     LVVFVGIVAMYVIMGGMRGVMYTDAFQGTVMFVSMTILLVTIYSSTGGIVAAHTKLTA
                     LAPQAVKIWGGAGHQGWTAMPVFWSPLWQQLVTTIVLGVGIGVLAQPQLVVRFMTVKS
                     GHELNRAVLAGGVFIMMMTGVAFVVGALANVYFFEQTENIALLAAGKKTDAIIPLFIK
                     DAMPWWFTSLFMITLLAAAMSTLSSQFHSMGSAFGRDFLEKGLKVKIKDSITVNKLAM
                     IAAILFSTLLAFGLPLFYQEGTAIIATGTAIFFGLCASAFLPMFVGALYFKRMSATAA
                     LASFWAGIGVSLFALIFIHIKESKPLGLCNLLFGTDTLIPVLQNLDPLIMALPVSVIT
                     AVVVTFFSKPPSQRVTDQAFKNIKK"
     gene            8899..9927
                     /locus_tag="P5123_01745"
     CDS             8899..9927
                     /locus_tag="P5123_01745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010260111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HRX15017.1"
                     /translation="MRKILIIGGTGTISSAVVKKAVEDGEEVFLLNRGNRISIFNDKR
                     INHFNCDINDTDTVNSLLGDTIFDVVADFISFVPEHLERNFFLFKKRTKQYIFISSAS
                     AYQKPLGHYLITEGTPLSNPYWEYSRNKILCEEYLINKYRAEGFPVTIVRPSHTYDER
                     SIPLGVHGKNGSWQVANRMFEGKPVIIHGDGTSLWTMTHNSDFAMGFNGLFGNIHAIG
                     EAVHITSDETVTWNQVYESIAAALGVELKAVHVASEFLALSGDYDLTGSLIGDKANSV
                     VFDNSKIKRLVPGFCAKTRFDTGIKQTIENVLSNKELRIEDPEFDRWCDHLISLYLKA
                     SADVRALA"
     gene            complement(9958..11088)
                     /locus_tag="P5123_01750"
     CDS             complement(9958..11088)
                     /locus_tag="P5123_01750"
                     /inference="COORDINATES: protein motif:HMM:NF024242.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HRX15018.1"
                     /translation="MIFKRKTLIYLSIILICAAIIVPDFINRGKNLVPSHSFSLTTIT
                     DQPDQGNSNISLYETDKGQISFKFKLNKGYTFPYAGIRLKSAKDYLFKTTNHDFLRIV
                     ANSDSACTIMINIITDVPIYDSKNGLYHRRAVYFSTMAKIEEGYNTINTALSDFQIPE
                     WWFTKNRIERSKAKNIRLKAFGNMEILVNSNNQSQQGNIVVKSISLEKKISNLLITNF
                     IKLFSITFFIVILVYFVKLRATFKKAKQTVLTHKKIHLDNEKERIKANIVNFLDQNYS
                     DPDLNISYMSKKTNISIYLIPSLLKEISDCTFKQYVNNLRVNKAKDMLKNEDTKIIDV
                     AYSVGYNTVTHFNKVFKSLEGISPKDYRKKYQSIKPEYSEQQ"
     gene            11388..12404
                     /locus_tag="P5123_01755"
     CDS             11388..12404
                     /locus_tag="P5123_01755"
                     /EC_number="1.1.1.94"
                     /inference="COORDINATES: protein motif:HMM:NF000942.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009331 - glycerol-3-phosphate
                     dehydrogenase complex [Evidence IEA];
                     GO_function: GO:0047952 - glycerol-3-phosphate
                     dehydrogenase [NAD(P)+] activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0046167 - glycerol-3-phosphate biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent glycerol-3-phosphate
                     dehydrogenase"
                     /protein_id="HRX15019.1"
                     /translation="MSKISVIGAGAFGTSLAMHCKSLGFDVRVWCFEKDLPGIVAEKG
                     ENTIYLPGYTFSKSVRFVNDPLEAVEGADLVLFVPPSAHLRKTAELFKGHITDKQLIL
                     TATKGIEHGSCMLMSQVMEDIYPEFRDNMAFLSGPSFAKDVAAGYPTDLSCAAFDIEV
                     AKKIQKMLHSSIIRIYTNDDVIGVELGGSVKNVLAIACGVSDELGFGLSARAALMTRG
                     LAEITRLGTAMGANPLTFQGLSGVGDLILTCTGDLSRNRTLGKKLAEGKTAEEIVSSQ
                     TAVAEGYVTAKPVVQLAEKYGIEMPISEAVYKVCYEGADLKKSVLKLMNRSEKDEFEG
                     INVC"
     gene            12470..13213
                     /locus_tag="P5123_01760"
     CDS             12470..13213
                     /locus_tag="P5123_01760"
                     /inference="COORDINATES: protein motif:HMM:NF015763.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRX15020.1"
                     /translation="MTLFVELMRSMDNRLNELLRNLSEQQSISVILILMLVSFAYGVI
                     HSMGPGHGKALVASFFLKEEHPLRRSLFLAAVISVIHTGSALVIAYLFNFVLTSVRGM
                     FRIKLQGYFIAVSGILIVCVGLFFLIRKFMKKKDEHVRSFEKKGNVFLVGFSAGIVPC
                     PAALMIMMFAFTAGIPLIGLYSVIFISLGMFVLLSVIGTLIIRSRKGIMDISSARFKK
                     SENVALFFEYFSIVAIICIGLIMSLRILI"
     gene            13210..13788
                     /locus_tag="P5123_01765"
     CDS             13210..13788
                     /locus_tag="P5123_01765"
                     /inference="COORDINATES: protein motif:HMM:NF017985.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1007 family protein"
                     /protein_id="HRX15021.1"
                     /translation="MNRGLTLFLRLFLTGIVLFISITAFSHPHLFIKPSLELFVEDGS
                     VSGIRIVWEWDKWWSAEVVAECDSDKNGVFDAKETKIVFEDFFSAVKSFGYFTAIYVD
                     GKSVKIDEVLQFAAFIDKDKIVTYSFVIPFAKPVKGTGASVKIDFNDETVYTAFDRGI
                     KIMPNDTFNVTDFKSSEHGFYGASVSFKVKGK"
     gene            13789..15624
                     /locus_tag="P5123_01770"
     CDS             13789..15624
                     /locus_tag="P5123_01770"
                     /inference="COORDINATES: protein motif:HMM:NF016190.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIM44-like domain-containing protein"
                     /protein_id="HRX15022.1"
                     /translation="MKVSAKRIIRASLFLIIIFSALYLYARAGGAGGASSGGSRGSSG
                     GSGDGELIFYLIYLLIRILISLPFPFNVISIGLILLVAAIVLNKGKKQVEEKSVFNNI
                     PTDKRPSKQGKIPSRFMADNPGFDQTQFLESVKNAFLQIQDAWMNKDLKNVRKFLSDG
                     VYQRFITQFKMMDLLDQTNRLSDITVNYTFIDKVESDSNYDVLHVAISASLKDNFICG
                     LDSSLNSGGYENFVEYWSFIRRKGVQKKGMFDKQSCPNCGASLEEGLGEVCVCPYCGS
                     LLNSGEYDWVLSEITQAVDYAGNKAGFFSSRSLRQQVKRLVLENSDFSVQFVEDKASN
                     GFLQYITALTLKEPAIMRRFVSDNLYESVKGNFDGRQIVYNRIYLNDVSLVGIIEEED
                     YNRLYVIIKYSFQRVELIDKKIRIVDPVVISDSKVVVMKRKKGVSPAKGSLYSHNCPN
                     CGGKLSDTLDIHCPYCSVVLNSSDIEWTVDDIMHLDKYRDFLDNNSSLFEYSVSIKTI
                     DSMYDVRDYALNNVLVVAAADGRLDESEKQFVDNLVKKWKYNPEKIKGLYDQAFSGNL
                     TIMMPDNTKKRQKIFELMCKAAAVDGDIDPQEQKILDEVRSYYLS"
     gene            complement(15673..17739)
                     /locus_tag="P5123_01775"
     CDS             complement(15673..17739)
                     /locus_tag="P5123_01775"
                     /EC_number="3.6.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012827074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium-translocating pyrophosphatase"
                     /protein_id="HRX15023.1"
                     /translation="MKMYNLVYLVPIGAILAIVFALFQTIKIKKSNTGNDKMREIAGY
                     IREGAMAFLKTVYTRLFFFVIIISILLVIGYYSKGNLMLTAVSFAVGAFLSALSGFLG
                     MRVATLSNVRTAEAARSNLNKALTVAFTGGSVMGMNVVGLALFGISILFIIYSNLFGF
                     NSMSLQNIVLPILSGFSLGASSIALFARVGGGIYTKAADVGADLAGKIEAGIPEDDPR
                     NPATIADNVGDNVGDVAGMGADLFESYAGSIISAMILGLATFDDKIEYVLIPLFIATA
                     GIIMSLACTFLVHTKENGDPQHALNKGTFSAAILTAASSIAIIKYFSVDAIIRGETIS
                     ASQMAIRLSVTTALGLISGVAIGLLTEWFTSKGRRWVMQIAKASEMGAATNIIKGLSV
                     GMYSTVLPILILAATIVISFNLAGVYGIAIAALGMLATTGIQLAVDAYGPIADNAGGI
                     AEMSELPKEVRERTDKLDAVGNTTAAIGKGFAIGSAALTALAFFSSFMKIAEIQSIDI
                     STPLVMAGVFIGGMLPFLFTALAMDAVGEAASEMIQEVRRQFHEIKGLMEGTAKADYA
                     RCVTISTQAAIKKMMLPTVIALVTPVLTGFWGGAEMLGGLLAGATVTGVLMALFQSNA
                     GGAWDNAKKSIEEGAHGGKTSTAHSAAVVGDTVGDPFKDASGPSLNILIKLLSVIALI
                     IAPMIV"
     gene            complement(17995..19353)
                     /locus_tag="P5123_01780"
     CDS             complement(17995..19353)
                     /locus_tag="P5123_01780"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase dimerization/phosphoacceptor
                     domain -containing protein"
                     /protein_id="HRX15024.1"
                     /translation="MINGNDLFLLMFSLSQSKEINTIKKQFIEAINSLSDNFSWSYGD
                     NPQGEIYFKIASGADSFGFLNLDKDSGKPDNLTLTNISNATALLAILLQNAVRSKLLE
                     QQNTELLSEINDKNIFLQNLINTVPSGLYLYHIDKNWFEILNPRFKEITGYTSKAADD
                     ESLIYLEKIIHPDDIKKLVEKHDILLKQKDDSIRISTFRINTESSGLKWIRTHDIPFK
                     RDKDGKLLTILGIADDITDEIMFTNQLEASLKEKQVLIREIHHRVKNNLQIVSSLLNL
                     QASSIRDTPYATFFDECRSRVMSMAHVHEQLYRYDNLYNINFDKYMFDFVKDIHNSFD
                     SDLFHVKIDIDCTNVMLPIDQAIPLGLIINEFLLNSYKHAFTDIKDSLISISITQVAD
                     QYTLIYKDNGKGYEDKDNTRPVPGLGLDLVNSLILQIRATFEHINHSGTQYKIIFSRH
                     TR"
     gene            complement(19466..20125)
                     /locus_tag="P5123_01785"
     CDS             complement(19466..20125)
                     /locus_tag="P5123_01785"
                     /inference="COORDINATES: protein motif:HMM:NF025774.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MEDS domain-containing protein"
                     /protein_id="HRX15025.1"
                     /translation="MKIKSSDQPELNLGFGDHVCNWGIHIAGLYENDTQRDDIIYGFL
                     NQGLIEKDIDLFCPVERSIDGFMDSFASHCGRKKSELLTNDNFQILPAKDIYYPNGTF
                     SPFEMDKNLNSFFEESQKNGPRNIRATAEMVWALEAVPGREHLFAYESRLNYFIPGKP
                     WVSICMYNTTKFSGSTIMDVLRTHPYTISNGILMQNPYFIDPDIWLAENAPQFLYSKD
                     S"
     gene            complement(20449..21783)
                     /gene="gdhA"
                     /locus_tag="P5123_01790"
     CDS             complement(20449..21783)
                     /gene="gdhA"
                     /locus_tag="P5123_01790"
                     /EC_number="1.4.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010964055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016639 - oxidoreductase activity, acting
                     on the CH-NH2 group of donors, NAD or NADP as acceptor
                     [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-specific glutamate dehydrogenase"
                     /protein_id="HRX15026.1"
                     /translation="MKLLANVMDQVQKRNPSEPEFHQAAKEVLESLEMVAEKNPQWIK
                     EGIFDRIVEPERQIMFRVPWMDDNGKVHVNRGFRVQFNSAIGPYKGGLRFHPSVNLGI
                     IKFLGFEQIFKNSLTGLPMGGGKGGSDFDPKGKSDAEIMRFCQSFMSELFRHIGENTD
                     VPAGDIGVGGREIGYMYGMYRKIRNDFTGILTGKGLAWGGSLARTQATGYGLCYFMDV
                     AMQAIKGKSFKGSTVVVSGSGNVAIYATEKVQELGGKVVALSDSAGYIHDPDGIKLDT
                     VKQIKEVERKRISEYIKHHPNAKYTEGCAGIWSIKCDIALPCATQNELDGEAAKTLVN
                     NGCFAVGEGANMPSTPEAVDLFIEKGIVFGPGKAANAGGVATSGLEMCQNSMRYSWSF
                     EEVDSKLKGIMEDIFKNASAAAKEYGQENNLVIGANIAGFKKVAEAMSAQGF"
     gene            complement(22264..22995)
                     /locus_tag="P5123_01795"
     CDS             complement(22264..22995)
                     /locus_tag="P5123_01795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013658431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HRX15027.1"
                     /translation="MILLENIKKNFGKLKVLKGVDLKVDEGEVVVIIGPSGSGKSTLL
                     RCINFLETAEKGSRISIDGKVIHRQKNDINMLRRNVGMVFQHFNLFPHMTVLQNVMEG
                     PTQVLHMKKDKARKQGIELLEKVGLADKHNAYPQMLSGGQKQRVAIARALSMNPQIML
                     FDEPTSALDPELVGEVIEVMADLAREGMTMIVVTHEMWFAKEAADRVCVMDDGLIIEE
                     GTPEQIFDSPTTERAQQFLQKIVVG"
     gene            complement(23008..23658)
                     /locus_tag="P5123_01800"
     CDS             complement(23008..23658)
                     /locus_tag="P5123_01800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004619425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HRX15028.1"
                     /translation="MGESLKAIITRFPTFWKAGLITLQLTSAGILFGLLIGLLFGLMR
                     VSEIKFMRYPAKAYIFLIRGTPLLLQLFIIYYGITSIITIRSFPSAVIALGIHNGAYI
                     AEIFRGAIQSIDKGQMEAARSLGMSKTKAMERIIMPQAFKRAIPSLGNQFIIALKDSS
                     LASTITISELLLRARQIGNTHFILMEMLIVVAIWYLILTSLFTYLIHRLEVHFKTS"
     gene            complement(23819..24568)
                     /locus_tag="P5123_01805"
     CDS             complement(23819..24568)
                     /locus_tag="P5123_01805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014186843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HRX15029.1"
                     /translation="MKLKLLILTLIIVLSASCAKKDENVIRFAGSGGYPPFNFMSEDN
                     EVIGFDVDVAHEIAKRMGKEMVYVTTSWDGIIEGLRAGRYEGILGSMAITEERLKVVD
                     FSTPYYYSGAQIIAKKDSSITGPDKLNKKHTVGLVTATTFAQDAEKLGVKINLYEDDN
                     QTLMELINGRVDAVITDRVVGLNAIKKIKGGSDLTLAGSLLRTEECAIALQKDSPLKD
                     EINKILDDMRKDGTLKSISTKWFDGEDITVK"
     gene            complement(25093..25362)
                     /locus_tag="P5123_01810"
     CDS             complement(25093..25362)
                     /locus_tag="P5123_01810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014256567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5320 domain-containing protein"
                     /protein_id="HRX15030.1"
                     /translation="MPGFNGTGPFGNGPMTGRGLGPCGQGRAAGYGYGRGMGFGRRGA
                     GFGFGARFFGAYERPYEPTLEELRQQRDLLEKQIESMQQQDNNNL"
     gene            complement(25450..25911)
                     /locus_tag="P5123_01815"
     CDS             complement(25450..25911)
                     /locus_tag="P5123_01815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020253411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF134 domain-containing protein"
                     /protein_id="HRX15031.1"
                     /translation="MSRPRKTRTIEGPPYCKSFKPQGIPTRYINTIVITVEEYECVRL
                     ADYNNLEHQDASSKLGISRSVFTRMLQNARNKIAKAIIDGCELLIEGGEFDFENNRIR
                     CLACYNTYQAPINEVSLTVCPNCSSDKLQNLNGQYAQRVVRGGRRHRGGRG"
BASE COUNT         7483 a         4623 c         5644 g         8285 t
ORIGIN      
        1 ctagtcttgg ttgcgtttta tgcaaatcat ggatatgtcc tcgaaagttc tgagtttatg
       61 tgagaaaaga gaagtctgtt ggattattgt atcaagtatc tcattgcttg ataaatcggc
      121 ttgaacaaga cttgcaagtc gcttaatgcc gaactgctca ccgtgtttat ttttaatgcg
      181 tgtcacacct tcagtgaaga ttattgcggt atcacctttt gaaaattcaa ttttagatct
      241 tttaatagtg cctgtattaa tatccgaatt tgccttgatc agtttgattt tccggtcttg
      301 cactagtatt aatctaatgt tgccagataa tgtgtattca aggcaatttc ggttggtgaa
      361 gagatgtgcg taaaaaatcg aagcttcttt cggaacagta atatctttgc ggcttgactg
      421 aaaataacga attacttccg agcagttgcg gcatactctg ccgaaagctg taattttccc
      481 ggcaatatat gaggcggtta ttgctcctgg tagggaataa ccccccgcat cggcaagcat
      541 tattgcgaca tgatcctttt cagaacggct tgtgaagatg tcaccgcaga attctccggc
      601 aggacggcaa atcattgtgg tatttatttc gtcacgttca atggttatcg gttttaaaaa
      661 aggttcaaaa agtttttgag cagagctgat ttcgtgctgc atttttgtgc gctcaatgga
      721 tttttcaaaa ataatggcgt tttgtacagc cataactgcc tgttcggcaa acagggtaaa
      781 aagcttcatg tcgttttctg taaacattac tctaccgata ggattcactc cctctattac
      841 gcctatcagt tttcccttga aaagcaaagg ggcacaaatt attgcatttg ttttgaaacc
      901 ggtaatcttg tcaaagttgg gattaaaacg ggggtctgag taaacatcat tggcaataag
      961 agcttctctg ttagcggcgc accatcctgc tatgccctgt gaagtcttga atcgatactg
     1021 ttcggagagc atatccgatt tttcaccggt tactgctttg aaaaccagct cgtctgtaag
     1081 ctcatcgtaa aagagaaggg agctggcttc agtatgcaaa atatctttaa tagccttcat
     1141 gacagctttt aaaagtttac caatgtcaag ggtcgcgttt atgagcttgg taatttcgat
     1201 taatttgcga aggttattgt aatctttctt tagagtgtca aaagaaaagt cactcatcgc
     1261 aaataccttc tgttgctaat aaaaaggctt cccacaaagg ttgtaaaaaa gaaaaacatt
     1321 attcttaata gtcggccgta acgccaaggt ttagtgatga tgcggtaaaa ccagtcaaga
     1381 ccacgctgca taaaaaaagc aggagctttt cggttttctc ctgaaatgat gtccagactt
     1441 ccaccaatac cgataattac agaattttgc aggttcattc ttaatttcga gatccaacga
     1501 tcctctttgg ggaaacccat tccaaccagt acaatattcg cttcagattt ccggatagct
     1561 tcgattacag attgttcacg ctgaggatta aaatagccgc catgccggcc tacaatacgt
     1621 attttgggaa aagatttgag tatgttggag taggcttttt cggttatttc tggttttgct
     1681 ccaacgataa agattgtata ctctttaatt tcggatagtc gtataagttc cattaaaaag
     1741 ctgatgaaat gtatacgctc tttgaggggt tgccttacta ttcgtgaaag tttgcgtatg
     1801 cccgcacctg tggcaaattt aagtgatgct ttgccataga ttatgttaag atcaatattg
     1861 gctctgtatc ttataagttt atatgggtta agtgaaataa catgatgaac accaccgtgt
     1921 tcgatcatct ccataatttt tacaatagcc tgttttgtgg aaacattgtc gactccaata
     1981 ccttttatat tggaaacagt tacgtcttca aggtcaacct gattgtattc gagtatcaaa
     2041 tcacggtttt ctttccatga atcgtagtag acatcttctg tgctcattgg tacctcataa
     2101 cctgaatact gttaagtaaa attataataa acggatatca gccggggttt atccaggtaa
     2161 ataaatatcc cacattagaa gattaggttg cctggactct aatctagaaa ttttatactt
     2221 gctttataaa agtttggtgt caacaataaa ttaagccgtc aaataatgat ttgcggtatc
     2281 aacagattgg agaatcagga tgaactatac agagaagatt tccaggtaca ttgagttaaa
     2341 tcttaaggaa aatcttacta ttgagcggga aatgtgcaat aaaaaacagc acgattttgt
     2401 gtcaaaggcc attgaaacat accgtccgga atccgtttta aaggctggtc ttggtaatgg
     2461 tgaattgctt ttgaaaatgg cagaggttgg aataaaagta acggttgttg agcccagtat
     2521 agaggtaatt aacaattttg taaaaaagaa caaagataat gttctaatag ataatattca
     2581 gtttattaat ggagatttga attcgctgcc tgtcgattat tacgcttttg gaatgatagt
     2641 ttgtatggat tatcttgatt ttcaaaatac tgcagctgtt tttgacgagt ttaaaagggc
     2701 aatagatttt gagggagtgc ttgtgcttgg aggtattgtt cttaataacg atgatattga
     2761 tggtatttat gatgatttat accatataat aatgccaatg cataatgatt tctatttgcc
     2821 aaccgattta aatacggttt tagggttaaa acatttttct tttatacaaa atgaagtttt
     2881 taatataccg gtagaactgg aaagttataa aagctttgct aaaaaatacg gagctgaatt
     2941 ttcattttca gaatacaaca aggcagatct atttattgaa gaaaaccgtg cacaattggc
     3001 aaaaatgtat gaatatgacg gcaatggggc agtagagcct tatcttttat cgctgtacaa
     3061 gagtgaaaaa cctgaaaaac aggagtatga aaaagaggta gaagattata agcgtaagac
     3121 aggacgggca ggagcctgat tctaaaagta tcggaaaaaa agcggttaaa aagtgttgac
     3181 ctgataggcc gagatttaat ctttaaaaag aattataatt agcttaagtc aaggctaaaa
     3241 ttttagttta ggtctattgt ggttaacata atgaactatg aaataaaata tgatactcgc
     3301 gacaatattg cttcgcttct ttctgaaaat aatccgaaac aagttgagct gatacgcaag
     3361 atggcgcacg atgttttact tgaaaattgt ggcagtggag tttactatcg cggacttata
     3421 gagttttcga accggtgtgt cagcgattgc tattattgcg gtattcgtcg ttctaacgcc
     3481 gatgtgaaca gattcgattt aaccatggac gagattgttg attcggcatt atggtgcgcg
     3541 aaaaacggtt atggctcgat agttttacag tctggtgaaa acagcagtcg ggattttatt
     3601 gattttgtct gccgtgctgt agaaacaata aagaaaaggt ctatttcgga agaacttccg
     3661 aatggtttgg gtgtaaccct ctgtataggt gagcagtcag aggcagatta cgcgagactc
     3721 ttcgctgctg gggctcatcg ttatctttta cgaatcgaga cttctaaccg ggagttgttt
     3781 aaaactattc atcctgaagc ccagtctttc gataaccggg ttgaatgtct gaggcggcta
     3841 aagcgaaccg gctttcaggt gggaaccggg gttatgtacg gtttacctgg tcagacttac
     3901 cagatgcttg ctgatgatat tctgtttttt aaagatatgg atatagacat gctgggcatg
     3961 gggccatata ttagacatag ctccaccccc cttaaaaaaa atgaaaacca gtttattatc
     4021 gagggagatg atcttttgcg tgtttctctt ctgatgatag cggcttgccg gcttgtgctt
     4081 aaggacataa atatcgctgc tacaacagct cttcagacaa tcgatccgac tggtcgagaa
     4141 aaggggctca gtttcggggc aaatgtggtt atgccacagc ttactcctgt tgattaccgt
     4201 aagagctatc ttttgtatga taacaaacca tgtcaggaag ataccaaaga ggagtgcaaa
     4261 aactgccttt ccgcgcgagt tggaagtgtt ggaagggaag ttcactacaa cgagtggggt
     4321 gactccccgc attttaaaag aaaaggggaa tcaaaaaatt gaaagaacta attgaacaga
     4381 ttaaacaata caagaagatt ctcggagaag atcttgttat gccagctcac cattatcaac
     4441 gccctgaagt tgtcgcgctg agcgatttcg cgggcgattc gtataaactg gctgttgaag
     4501 cctccagaca tacttcggac tttattctat tttgtggtgt tcgttttatg gcagagggag
     4561 ccagtgtttt ggctgccgat aaccagaaga tagtcgtgcc cgagcttttg gcggggtgcc
     4621 ccatggcgga tatgatagac aaatcgatgg ctcaaaaggc ttatgatata attagtcctc
     4681 tctcaaacag gagaatcgct cctgtagttt atatgaattc gtatgcggat gtgaaatctt
     4741 tttgtggtga aaaggatggt gctgtatgta ccagctcgaa tgcgctgaaa atatgttcgc
     4801 atttcattga gaagggtgag gctgttttct tttttccgga ttacaatctt ggtataaata
     4861 ctgctgatac tcttggtctc gaagattatg aggtagtgcg tattggacgc gatcttgtca
     4921 tcgaagaacg tgtcgattac aagaatgtga agatgtatct ttgggatggt ttttgtcatg
     4981 ttcataaggt atttactgtt gatgatgtta aaaaagcacg cgaacaatat tctggaatca
     5041 aaataatagt tcatcccgaa agtgacagag cggttgttca agcatcagat atgaatggct
     5101 cgactgcatt tattcttgat agcataaggg attcagaacc cggttctgtt tggggagttg
     5161 gtaccgaagt aaattttgta agacgtatag cttcggattt tggcccaaac agtactcata
     5221 acaagacaat tgtagccctt cgtgactctc cctgttttaa tatggaaaag acttcacttg
     5281 aaaaggttgc tgcttcttta aaatccatag ttgattataa aaatggagag ggtgaactgg
     5341 ttactgagct cagagtgcaa ccagaattta aggatggagc caaaaaggct ttgcagacga
     5401 tgatggatat tgttcaaaaa gcctagaccg tttcgtgagg gcctgaagct gtgtcgtcta
     5461 cagtgccgct tatcgatttg gcggtactaa ctttaaccgg gacatctttt ataaagatta
     5521 ctgttaaaat taaagctatt atcgatagac ctgtgggcac catccagaac attgtatagt
     5581 cggttgtacc attttttgaa tagtagtcag tcagaaaacc cgctgttatt gtgcctgtaa
     5641 gccccgagaa cccaaaaaat atcatgttaa ttatctgatg tactccggat ctgttctctt
     5701 cggtgcactg cgtatcgaca taaattgttg ctgttgcgta aaagaaggtg taaataaacc
     5761 cattacatag aagtgcagta actatcccaa gtttgcttcc tgtaagtgta aatatggtgt
     5821 agcgcgttac ctgaaaagca agacccaaaa gaaaactctt tttaaagcca atacccttga
     5881 aataaaaaga tcctccttta tggttaatca aaaaaccacc caaaataaac ccaagaaccg
     5941 gaatagaaac tatttccaga acttgtccga gtgtttgcat ggcaagatta tttccgtacg
     6001 agtaacccag atgttccaga tatggccctg tgccgaaaaa gtaaaactga tccattgtgc
     6061 ttgaaaggaa aaagactact gtaagcgata atagctctgg ccgggctatt gttttaaatg
     6121 cgcttgcggg aattatatcc ttgatgctct ttctgctacg tggtggcagg ggaatatccg
     6181 gtaaaaagaa acagattatg ctgataatga tgcccataat ggaggctgca aaaaagatat
     6241 gagatactgg gccattgaaa aatttaatat atagctgtcc caaaatcagt ccggacgcca
     6301 tccaacccaa agttccgaaa accctgatag aaccgaagtg ctcacgtctg gacttgagat
     6361 ttttaaatgt aactgcatta acaagcccca tcgtaggtgc gaagagtaac atgtaaaaca
     6421 gataagagaa gagtacagat ttaaaagagg tattgatggc cgtaaaaaaa ctgaagaacg
     6481 cggcagtgaa atgaagtatg gataaaagat gtcttgcttt aattagcctg tcgacaacaa
     6541 aacttgcggc aagaggagaa aatataccag ctattacagt ggttgagatt attaccgaag
     6601 cctgtgttcc gctgaagttg agtactttgg tcagataggt gcttagtatg ggacggtaag
     6661 agccgtaaat cgcgagttgc aaaaacatga taattgccag aagtgagaaa ggatacagtt
     6721 ttttcataat aagagatgta actctgccgt taatttttga agatcataag ataagaggtt
     6781 ccgataactg actgaaactt taaaattata ttcggggtga ctggatttga accagcggcc
     6841 ccttcgtccc gaacgaagtg cgctaccgga ctgcgctaca ccccgaataa ttaatatctg
     6901 ttttttagtt ttcaggtgtc aagtttattt gtctgctctg tattttgaac ttggtaaaaa
     6961 aataaaaaaa gaggttagta cacagattat ttttcaatag ttgacataat tcaaattatg
     7021 tataaaaatg ccagttaaag accggctggt cggacggctg atctatatta aaagaggagg
     7081 tattgccatg ctgggttttc aggattgggg ggttaccctg gcttacgtag gcacaatagt
     7141 ctccgcggtt atatgtgtag tgtacggtat agtgaattgg aatagaccgc gtgaagatga
     7201 agagtcagaa atcaatgaag aacttgagtg ggaaaaaaat gatccggagc tcaaagaagg
     7261 aaatatataa tgaatatgat ttttttgggg tttgttgtta ttctttatct ttctgtaatc
     7321 gcttttttgg gttatcgtgg atacaaacgc acaaaaagca catctgacta tttgcttgcg
     7381 ggtcgtaaaa tacatccaat gataatggca atatcttatg gagcaacttt cataagtacc
     7441 tccgcaatag ttggttttgg cggtgccgca gctgtttttg gtatgggagt tttatggtta
     7501 actgtattga atatttttgt tggtatattt atagcctttg tatttttcgg tttgcgaaca
     7561 agaaaaatgg ggcacaatct tgacgcacat acctttccgg aactgcttgg aagaagattc
     7621 gattcccagt ttttacagac ggccggaggc ttgctgatat ttttggcgat gcctttatat
     7681 gccgctgcaa ttattctggg tggagcacag tttatgtcca caaccctggc aattccgtat
     7741 gaggtttcgc ttgttgtttt tgtgggtatt gtagcgatgt atgtcattat gggtggtatg
     7801 cgcggtgtta tgtataccga tgcttttcag ggtaccgtca tgtttgtcag catgacaatt
     7861 cttcttgtca ctatatactc ttccacagga gggatagttg ccgcgcacac aaaacttacc
     7921 gctttggctc cgcaggcagt aaagatctgg ggaggtgcag gacatcaggg gtggacggca
     7981 atgccggttt tttggtcccc gctttggcag caactggtaa ctacaatagt gcttggtgtt
     8041 ggtatagggg ttttggctca gccacagctt gtcgttaggt ttatgacggt aaagagcggt
     8101 catgaactaa atagggctgt tcttgctgga ggagtcttta ttatgatgat gactggtgtt
     8161 gcttttgtag tgggagcgtt agcaaatgta tatttctttg agcaaaccga aaatattgcg
     8221 cttcttgcgg ctggtaagaa aactgatgcc attataccat tgtttatcaa agatgcaatg
     8281 ccgtggtggt ttacttctct ttttatgatt acacttcttg ctgcagccat gtcaacttta
     8341 tcgagtcagt ttcattctat gggatcggct tttggtcgag attttcttga aaagggtctt
     8401 aaagtaaaaa ttaaggattc aataacagta aataaacttg caatgattgc agcgattctt
     8461 ttttcaactc ttttagcctt tggattacct ctgttttatc aagagggaac agcaattatt
     8521 gcaactggaa ccgctatatt ctttggatta tgtgcttcag catttttacc gatgtttgtc
     8581 ggagctctct attttaaacg aatgagcgct actgcggccc ttgccagctt ctgggcaggt
     8641 attggagtaa gtcttttcgc gttgattttt atacatatca aggagtcaaa acctcttgga
     8701 ttatgtaatt tactctttgg tactgatacg cttatacctg ttttgcagaa ccttgatcct
     8761 ttgattatgg ctttgccggt atctgtaatt acggctgttg ttgttacctt cttttctaag
     8821 cctccttcgc aaagagtaac tgatcaagcg tttaaaaata ttaaaaagta gaaatatgag
     8881 caaacttaag gaatagcaat gagaaaaata ttgataattg gcggtactgg caccataagt
     8941 agtgccgttg ttaaaaaggc tgttgaggat ggggaagagg tttttctgct taacaggggt
     9001 aaccgaattt caatttttaa tgataagcga ataaatcatt ttaattgcga tattaatgat
     9061 actgatactg ttaattcttt actgggagat accatatttg atgtggtggc tgactttatc
     9121 tcatttgtgc cggagcatct tgaacgtaac ttctttttgt ttaaaaaacg tacaaagcag
     9181 tacatattta taagttcagc ttcagcatat cagaaaccgt taggtcatta tcttataaca
     9241 gaaggaacgc cgctaagcaa tccatattgg gaatactcta gaaacaaaat attgtgcgag
     9301 gagtatttga ttaacaagta tcgggccgaa gggtttccgg ttaccattgt tcgccctagc
     9361 cacacctacg acgaaagatc gataccactt ggggttcatg gtaaaaatgg tagttggcag
     9421 gttgcaaacc gaatgtttga aggaaagccg gtcattattc atggtgacgg aacatcattg
     9481 tggaccatga ctcataatag tgattttgcg atgggattta atggcctttt cggaaatata
     9541 cacgcaatag gtgaagcagt tcatataact tcagatgaaa cagtaacatg gaaccaggtt
     9601 tatgaatcaa ttgccgctgc acttggtgta gagttaaagg ctgttcatgt cgcatcagaa
     9661 tttttggctc tatcaggtga ttacgattta acaggatcgc ttattggcga taaagcaaac
     9721 agtgttgtat ttgacaatag taaaataaaa aggctggttc ctggattttg cgccaagacg
     9781 aggtttgata caggtatcaa gcagactatt gagaatgttt taagtaataa agaattacgg
     9841 atcgaggatc ccgagtttga caggtggtgc gatcatctaa tttcgttata tttaaaggct
     9901 tcggccgatg tcagagctct tgcttgaatt ctgtagcagt agagcagtta agctgctcta
     9961 ctgttgttcg gaatactcag gtttaatgga ttgatatttt ttacgataat cttttggaga
    10021 aataccttca agagatttaa acaccttatt aaaatgagtt acggtattat atccaaccga
    10081 ataggcaaca tcaataatct ttgtatcttc gttcttaagc atatcttttg ccttgttaac
    10141 ccgcagattg tttacgtatt gcttgaaagt acaatcagat atttctttaa gaagcgatgg
    10201 aatcagatat attgatatat ttgttttttt agacatataa cttatattta gatctggatc
    10261 tgaataattt tggtcaagaa aattaactat gtttgctttg attctctctt tttcgttgtc
    10321 taaatgtatc tttttatggg ttaatactgt ttgtttagct ttcttgaaag tggctctaag
    10381 tttaacaaaa tataccagta taacaataaa aaatgtaatt gaaaatagtt ttataaaatt
    10441 tgtaattagc aggttcgaaa tttttttttc gagggatatc gatttaacta caatgttgcc
    10501 ctgttgtgac tggttgtttg agttaaccag tatttccatg tttccaaagg cctttagtct
    10561 gatatttttg gccttgcttc tttcaatacg gttttttgta aaccaccatt cgggaatctg
    10621 aaagtcggat aatgctgtat taatggtgtt ataaccctct tcaatttttg ccatggtgct
    10681 gaaataaacg gcacgtctgt ggtaaaggcc attttttgaa tcataaatag gtacatctgt
    10741 tatgatgttt atcattatag tgcatgccga atcagagtta gcgacgatac gtaaaaaatc
    10801 atgatttgtt gttttgaata ggtagtcttt agctgattta agccgaatac cagcatatgg
    10861 gaaagtatag cccttgttga gtttgaattt aaacgaaatt tgccctttat ccgtttcata
    10921 taaactgatg tttgaatttc cttgatcggg ctgatcggtt atagttgtca aagaaaaaga
    10981 atgagaagga acaagattct ttccacggtt tataaaatca ggtactatta ttgcagcgca
    11041 tattaatata atagataaat aaatcagagt ttttctttta aatatcatgc tgggaatcct
    11101 gaatttctaa tctttgtatt cagacggtgg ctttgtttga aacattattt atgagattat
    11161 taaacttgta aaaaacaaga ataatttacc aacttcattt ttttggaaat tttatacttg
    11221 attccttgat tacctgctct taaaacaaat aggtctttat ctttaaatta ctattatatt
    11281 atcataaata ccaaatattg gcagcttttt gtgaaaattc cttcgtaagc aagttgtagt
    11341 ttttagtttt ggttttaaga ctaattaaaa aaaaagcgga gttaaatatg tcaaaaattt
    11401 ctgttattgg tgccggcgcg ttcgggacct ctttggccat gcattgtaaa agtcttgggt
    11461 tcgatgtaag agtctggtgt tttgaaaagg atttaccggg aattgttgcc gaaaaaggcg
    11521 aaaataccat ttatttgccc ggttatacat tttcaaagtc tgtgcgcttt gttaatgacc
    11581 ctcttgaagc cgttgaggga gcagatcttg tactctttgt cccgccttcg gcacatttaa
    11641 gaaaaacagc agaactgttt aagggacata taaccgacaa acagctgatt ctgaccgcta
    11701 caaagggaat tgaacatgga agctgtatgc tgatgtctca ggttatggaa gatatttatc
    11761 cggaatttag agacaatatg gcatttttat ctggaccgtc gtttgctaaa gacgttgcgg
    11821 ctgggtatcc gacagatttg tcgtgtgcgg cgtttgatat tgaagtcgca aagaagattc
    11881 agaagatgct tcattcgtcg ataataagaa tttatacaaa tgatgatgtg ataggtgttg
    11941 aacttggagg atcagtcaaa aatgtgttgg caatcgcttg cggtgtcagt gacgaacttg
    12001 gttttgggct atcggctcga gccgcattaa tgacaagagg gttagccgag ataacaaggc
    12061 tgggtacagc tatgggggca aatccgctta cgtttcaggg gttatccggt gtgggtgatc
    12121 ttattttgac atgtacaggt gatctttcgc gaaaccgtac tttgggcaaa aaacttgccg
    12181 agggaaaaac cgcagaggaa attgtctctt cgcaaacagc tgttgcggaa ggctatgtta
    12241 ccgcaaagcc ggtggttcaa ttggctgaaa aatatggaat tgagatgccg atctcagaag
    12301 ccgtgtacaa ggtctgttat gagggagccg atttgaagaa gtccgtattg aaacttatga
    12361 acagatcgga aaaggatgaa tttgaaggta taaatgtctg ttaaatggtg aaaggtgcat
    12421 gatatgagtt tttgcctatg atggtatctc tggtaaaagg agaaatgtaa tgacattatt
    12481 tgtagaacta atgcgaagta tggataaccg tttaaatgaa ttgctgagaa acctgtctga
    12541 acagcagagc atttcggtta tactgatact tatgttggtc tcgtttgctt acggtgtaat
    12601 acactcgatg ggcccggggc acggcaaggc gctggttgct tcgttttttt tgaaagagga
    12661 gcatccattg aggcgttcgc tttttttagc agctgttatt tcggttatac ataccggatc
    12721 agcattggta atagcttatc tttttaattt tgttctgaca agcgtgcgag gtatgtttag
    12781 aataaaattg cagggttact ttattgcagt gagtggtatt ctgattgtgt gtgtgggcct
    12841 ctttttcctg atacgtaagt ttatgaaaaa gaaggatgag catgtcaggt cgtttgaaaa
    12901 aaaaggaaat gtctttttgg taggtttttc cgccggcatt gtgccttgcc ccgctgcatt
    12961 gatgatcatg atgtttgcct ttactgcggg cattccgctt ataggtttat attctgtaat
    13021 attcatctct ttgggcatgt ttgttctatt gtctgtgatt ggcacattaa taataaggtc
    13081 cagaaaagga attatggata tctcttctgc cagattcaaa aagtcagaga atgtggctct
    13141 cttctttgag tatttttcca ttgtcgctat tatatgtata ggtttgatta tgagtttgag
    13201 gatattgata tgaatcgtgg tttgactctg tttttgcgct tgtttctgac cggaattgta
    13261 ctgttcataa gcatcactgc tttttcacat ccgcatcttt ttattaaacc atcactagag
    13321 ctttttgttg aggacggttc ggtttcggga atacgaattg tctgggaatg ggataagtgg
    13381 tggagtgccg aagttgttgc cgagtgcgat tcggataaaa atggtgtgtt tgatgctaaa
    13441 gagacaaaga ttgtttttga ggatttcttt agcgctgtga aatcgtttgg ttactttact
    13501 gcaatttatg ttgatggcaa aagtgtaaaa atagacgagg ttttgcaatt cgcggccttt
    13561 attgacaaag ataaaatagt tacctactct tttgttatac ctttcgcaaa gccggttaag
    13621 ggaacaggtg cgagtgtaaa aatagatttt aatgatgaga ccgtttacac cgcctttgat
    13681 cgcggaataa aaataatgcc taatgataca tttaatgtaa ctgattttaa atcatctgaa
    13741 cacggattct acggggcatc ggtatcattt aaggtaaaag gaaaatagat gaaagtttct
    13801 gctaaaagaa ttattcgagc gtcactcttt ttaataatta tcttttcggc tctgtatctg
    13861 tatgccaggg ctggtggagc aggtggtgcg tcttccggag gaagcagggg ctcctcggga
    13921 ggaagtggcg acggcgaact gatattttat ctgatctatt tattgataag aattttgata
    13981 tctctgcctt ttccatttaa cgttattagc ataggattaa ttcttttagt cgcggctatt
    14041 gtacttaaca agggcaaaaa acaggtcgaa gaaaagtccg tctttaacaa tatacccact
    14101 gacaaaagac cttcaaagca gggaaaaatt cccagtcgct tcatggccga taatccgggg
    14161 tttgaccaga cgcaatttct tgaaagtgta aaaaacgcat ttttgcagat tcaggatgcc
    14221 tggatgaaca aggatttgaa gaatgtcaga aaatttttat ctgacggtgt gtatcagcga
    14281 tttattactc agttcaagat gatggatctt cttgatcaga ctaaccgatt gtcagatatt
    14341 actgtgaatt atacttttat tgataaggtt gaatctgata gtaattatga tgttttacac
    14401 gttgccattt cggcgtcgct aaaggataac tttatttgcg gccttgattc ttcattgaat
    14461 agcggtggct atgaaaattt cgttgagtac tggtctttta taaggcgtaa aggtgttcaa
    14521 aaaaaaggta tgtttgacaa acagagttgc ccgaattgtg gtgcttctct tgaggagggt
    14581 ttgggagaag tttgtgtttg cccttactgt ggcagtcttt tgaacagtgg tgaatatgac
    14641 tgggtgcttt ctgaaataac gcaggctgtc gattatgcgg gtaataaagc cggattcttt
    14701 tcaagccggt cacttcgtca acaggttaaa aggctggtac ttgagaatag tgatttttcg
    14761 gtacaatttg tggaagataa ggcaagcaat ggatttttgc aatatataac tgctctgaca
    14821 ttaaaagaac ctgctattat gagacggttc gtttctgata atctttatga aagcgttaag
    14881 ggtaattttg acggaagaca gatcgtttat aacagaattt accttaacga tgtatctctt
    14941 gtcggaataa ttgaggaaga agattataac aggctgtatg ttattatcaa gtattcgttt
    15001 cagcgagtcg aactaattga taaaaagatt agaattgtgg atcctgttgt gattagtgat
    15061 tcaaaagtag ttgtaatgaa acgcaaaaaa ggcgtctctc ctgcgaaggg ttctctctat
    15121 tcacataatt gtccaaactg cggaggcaaa ttaagtgata ctctggatat acattgccct
    15181 tactgtagcg tagttcttaa cagctccgat atcgagtgga ctgttgacga tataatgcat
    15241 cttgacaagt atagagattt tctggataac aacagctctc tttttgagta cagtgtatct
    15301 attaagacga tagactcaat gtacgatgtg agggattatg ccttaaacaa tgttcttgtt
    15361 gttgcggcgg cagatggaag acttgatgaa tcagaaaagc agtttgtcga taatcttgtg
    15421 aaaaaatgga aatataatcc ggaaaaaatt aaaggattat acgatcaggc attctctggc
    15481 aacttgacga ttatgatgcc ggacaacaca aagaagcgac aaaaaatatt tgagctcatg
    15541 tgcaaggccg cggctgtaga cggagatata gatcctcagg aacaaaagat actcgatgaa
    15601 gtgcgaagct actatctttc ataattacga gttttttgat agcggcttca ccctttaatc
    15661 ttttttatac gattatacaa tcattggagc tattatcagg gctataaccg ataacagttt
    15721 tattagtatg ttaagcgaag gtcctgatgc gtctttaaac gggtcaccaa ctgtatcacc
    15781 caccactgcc gcagaatggg cggtgctagt tttgcctccg tgggcgccct cttcgatgct
    15841 cttcttcgcg ttatcccagg ctccacctgc gtttgactga aaaagagcca taaggacgcc
    15901 agtgacggta gctccggcca gaagtccacc aagcatctct gctccgcccc aaaaaccggt
    15961 tagtacagga gttacaagag caattactgt tggtaacatc atttttttta tggctgcctg
    16021 tgttgaaatt gtcacacatc gtgcataatc ggctttggca gtcccctcca taagaccttt
    16081 aatttcatga aattggcgac gaacttcctg aatcatttcg gaagctgctt ctccaaccgc
    16141 gtccatggcc aaagcggtaa aaaggaaagg tagcattcca ccgataaaga ctccggccat
    16201 cacaagaggc gtggagatat caatggattg aatttctgcg attttcataa aagatgaaaa
    16261 gaaagcaagg gccgtaagag cggctgaacc gatagcaaaa ccttttccta ttgcagcggt
    16321 tgtattacct acggcatcaa gtttgtctgt acgttcacga acctctttgg gaagctcact
    16381 catctctgca attccaccag cgttgtcggc aatagggccg tatgcgtcta ctgccagttg
    16441 aatccctgtt gttgcaagca tgcccagcgc agcgatagct atgccatata ctccggcaag
    16501 attgaaagat atcacaatgg ttgcggcaag aataagtatg ggtaaaactg ttgagtacat
    16561 tccaacggat agccctttta taatattggt ggcagcgccc atttcactgg cctttgctat
    16621 ctgcattacc catctgcgac cttttgatgt gaaccattca gtcaagagcc ctattgcgac
    16681 accagatatt agaccaagtg ctgttgtaac gcttagccgt attgccatct gagatgccga
    16741 aatagtttcg cctcggatta tggcatcgac tgaaaaatat ttgattatgg ctatggatga
    16801 cgcagcagta agaattgcag cactaaaagt tcctttattt agggcgtgtt gagggtcacc
    16861 gttttccttt gtatgaacaa gaaaagtgca ggcaagtgac attattattc cagcggtagc
    16921 aataaagaga ggtattagaa catattctat tttatcgtca aatgtggcaa gacccagtat
    16981 cattgctgag ataatagacc cagcataaga ttcaaaaagg tcggctccca tgcctgcaac
    17041 atcacccacg ttgtctccta cattgtctgc aatagttgcg ggatttcgcg gatcatcttc
    17101 gggaataccg gcttctattt tgccggcaag atcggcaccg acatcggctg ccttggtata
    17161 gattccacca cctacgcgag caaaaagagc tatggaagag gcaccaagtg aaaatcccga
    17221 tagaatgggt aatacaatgt tttgcaaaga catggaattg aatccgaata aattggaata
    17281 tattataaaa agaatggata tgccgaatag agccagcccc acaacgttca tgcccattac
    17341 tgatccgccg gtaaatgcta cagttaatgc tttattgaga ttgcttcttg cggcctcggc
    17401 tgtacgtaca ttgctaagtg tagctacacg catgccaaga aatccggaca aagctgatag
    17461 aaaagcacca actgcaaaag agacggcagt tagcattaga ttgccttttg aataatagcc
    17521 gattacgagt aagatggata taattataac gaagaaaaac agtcgtgtat atacagtctt
    17581 tagaaaagcc atggcgcctt ctcgtatgta gccggcgatt tcgcgcattt tgtcgttgcc
    17641 agtattagat tttttgattt tgattgtctg aaaaagagca aaaacaatcg cgagtattgc
    17701 gccgataggt acaagataaa caagattata cattttcatt ttcctttata tatgggcttt
    17761 aaacggcatt gccattatgg aaatggtaat ttttcgaagt tcccttatgt tttaataatg
    17821 tgggcaaact ttcatgtttg tgttagccga tatattgata cagtttaatc gattaagtat
    17881 gatttgaaaa caaaatcgat atttggaagt aaaaataaca tcgcaaatgt acagcgtcaa
    17941 cttcttttga ggcggtatta tgattatggc tttgagcagt tgtttttgaa gaaattagcg
    18001 ggtgtgccgt gaaaagatta ttttgtattg tgtgccggag tggtttatat gttcaaatgt
    18061 ggctctaatt tgaagtatga ggctgttaac tagatcaaga ccaagcccag gaactgggcg
    18121 ggtgttgtct ttgtcttcgt aacctttgcc attatcttta tatattagtg tgtattgatc
    18181 agcaacctga gttatcgata tgcttatgag agaatctttg atatctgtga atgcgtgttt
    18241 gtacgaatta agcagaaact cattaattat tagaccaaga ggaattgcct gatcaatggg
    18301 aagcattaca tttgtgcagt cgatatcaat ttttacatgg aataaatcag aatcaaaact
    18361 attatgaata tccttcacaa aatcaaacat atacttatca aaattgatat tgtaaaggtt
    18421 atcatagcga tagagctgtt catgaacgtg cgccatgctc ataacccggc ttcggcactc
    18481 gtcaaaaaat gttgcgtagg gtgtatccct gattgatgat gcctgaaggt taagcaggct
    18541 tgaaacaatc tgaagattat tttttacgcg atggtgaatt tcccgaatta gtacctgctt
    18601 ttctttaagg gaggcttcaa gctgatttgt aaacattatt tcgtcagtaa tatcatctgc
    18661 aattccaagt attgttaaaa gcttgccgtc tttatcacgt ttaaacggga tatcatgcgt
    18721 acgtatccat tttaagcctg atgattccgt atttattctg aaagtggata tgcgtattga
    18781 atcgtctttc tgttttaaaa gtatgtcatg cttttcgacc agttttttaa tatcgtcggg
    18841 atgaattatt ttttccaggt atatcagaga ttcatcgtct gccgctttgg aagtatagcc
    18901 tgttatctct ttgaaacggg ggttcaggat ttcgaaccag tttttatcaa tatgataaag
    18961 gtaaagcccc gagggaaccg tgttgataag attttgtaaa aaaatattct tgtcgttgat
    19021 ttcgcttaga agttcagtat tttgctgctc aagcaatttt gaacgaaccg cgttttgcag
    19081 cagtattgca agtaaggcag tggcattgga aatatttgtc agagtcaggt tgtccggttt
    19141 tccggaatct ttatcaagat tcaagaaacc aaatgaatcg gctccggagg caattttaaa
    19201 ataaatttca ccttgcggat tgtcgccgta actccagctg aagttgtctg ataatgagtt
    19261 tatggcttca ataaactgtt tctttatagt attgatctct tttgattgag agagactgaa
    19321 cataagcaga aagagatcgt ttccgtttat catgattacg ccggctcctg ttttttgata
    19381 ctgcaaataa tctttataaa aaaacgtaaa tcacgggtta cgttaataat aaaacagccg
    19441 tgattacttg tggaaaaccg gcattttagc tgtctttgct gtacagaaat tgtggagcat
    19501 tttcggccaa ccagatgtcc gggtcaataa agtaggggtt ttgcataagt atcccgttag
    19561 atatggtgta aggatgagtt cgtaacacat ccatgattgt agagcctgag aactttgtgg
    19621 tgttgtacat gcagatgctg acccaaggct ttccgggaat aaagtagttc aatcttgatt
    19681 cgtaggcgaa aaggtgctcg cgtcctggaa ctgcctcaag tgcccatacc atttcggctg
    19741 tagctcttat attcctggga ccgttttttt gcgactcttc gaaaaaactg ttaagatttt
    19801 tgtccatttc gaaaggagaa aatgttccgt tcggataata gatatctttg gctggcagta
    19861 tctggaaatt gtcgtttgtt agaagttcac tctttttgcg gccgcagtgg ctggcaaaag
    19921 aatccataaa gccatctatt gaccgctcta cagggcaaaa aaggtcgata tccttttcaa
    19981 taagaccctg attcaaaaag ccgtaaatta tatcgtcacg ctgtgtatcg ttttcgtaaa
    20041 gaccggctat atgtattccc cagttacaaa catgatctcc aaaaccgaga ttcagttcgg
    20101 gttgatctga tgattttatt ttcatgtcta tcctcccctt attactataa tataagtaat
    20161 atttaaggtt taatcaaggt aatacccgca ataattttaa aactttattt ataccaggtg
    20221 caaaaaaaaa taacctttaa tatcaattaa gagatgagcc cttttgaatc aggttatttc
    20281 tttgaaattt tctgtggaca catcaataaa ttatagtgac gaggtgactg gttgtcaaaa
    20341 tttgagagaa attctgctta aatgtgggta aaaaattaaa aggaggatct tttgatcctc
    20401 ctttatgaga tatttaacta attgcttaag tctgaatcag aaatcagatt agaatccttg
    20461 cgcagacata gcttcggcaa cctttttaaa gcccgcgatg ttcgctccga taacaaggtt
    20521 gttttcctga ccatactctt ttgcggccgc gcttgcgttt ttaaaaatgt cttccatgat
    20581 gcctttcagt tttgaatcta cttcttcgaa agaccatgag tagcgcatgc tattctggca
    20641 catttcaaga cccgatgtcg ctacaccacc tgcgttagca gctttaccag gaccaaatac
    20701 gatacccttt tcgataaaaa gatcaacagc ttcaggagta gaaggcatgt ttgcgccttc
    20761 gcctacagca aaacagccat tgttcacaag tgtttttgct gcttcgccat caagttcgtt
    20821 ttgtgttgcg cacggaaggg caatgtcaca ttttatgctc cagatgccag cgcaaccttc
    20881 tgtgtatttc gcgtttggat ggtgcttgat atattcactg atacgttttc tttcgacttc
    20941 tttgatctgt tttactgtat caagtttgat tccgtcagga tcatggatgt atccggctga
    21001 atcacttaga gcaacgactt tgcctccaag ttcctgtacc ttttcagtgg cgtaaattgc
    21061 aacgtttcct gaacctgaaa caacaaccgt agagcctttg aatgatttac ctttgatagc
    21121 ttgcatggct acgtccatga aatagcagag accgtaacct gtagcctgtg ttcgggcaag
    21181 gcttccaccc caagcaaggc cttttcctgt tagtatgcct gtgaaatcgt ttctgatttt
    21241 acggtacatt ccatacatat aacctatctc tcggccgcct acacctatat caccggcagg
    21301 tacatctgtg ttttcgccta tatggcgaaa aagctcactc ataaagcttt gacaaaaacg
    21361 catgatttcg gcatcggatt taccttttgg gtcaaaatcg ctaccgccct tgccgccgcc
    21421 cataggaagg ccagttaaag agtttttgaa aatttgctcg aagcctaaaa atttgataat
    21481 gccaagatta actgaagggt gaaaacgaag accgcccttg taaggtccaa tggcgctgtt
    21541 aaactgaaca cgaaaacctc tgtttacatg aactttaccg ttatcgtcca tccaaggaac
    21601 acggaacatg atttgtcttt ccggttcaac gattcggtca aaaattcctt ccttgatcca
    21661 ctgaggattc ttttcggcca ccatttcgag tgactctagt acttcttttg ctgcctgatg
    21721 aaattcaggt tcgcttgggt ttcttttttg aacctgatcc ataacgtttg ctaaaagttt
    21781 cattattttc tcctaattta aaggtagtgc tgcaatataa acagaaatag tacgattagg
    21841 tattgattct gtttgagtga taaatgttaa gaataagtaa aaaacgttta ttattgaaac
    21901 atattggaac aatttaacat gcaattaaca ataatagggc atgatgcaaa caatgagcac
    21961 aatgtgaaga aatatttttt ttaattgtta cataaatgtt aaattaatca aagagaataa
    22021 acaagagatg tatccgggta ttatgctttg gttaactaaa gatactgtga tgaaacgatt
    22081 tggttttgtg aaaaaataaa tctgtgtttg gggcgtgttt ggtgccgaat gattatgtaa
    22141 ataatgttcg cgtagtaaaa atttcctgat tataaagctc ttaacaacga ccatagaatc
    22201 attttggatt tcaagtcgtt gttaagtaaa tcttgtaagt tgcattcaag ttatttattt
    22261 gagttagcca accacaattt tttgtaaaaa ttgctgagct cgctcagttg ttggtgaatc
    22321 aaatatttgc tcgggtgtgc cttcttcaat aatcagaccg tcgtccataa cgcatacacg
    22381 atcggctgcc tcttttgcaa accacatttc gtgtgttaca acaatcatgg tcattccttc
    22441 tcttgccaga tcggccataa cttcgataac ttcgccaaca agctccggat caagagccga
    22501 tgtgggttca tcaaacagca ttatttgcgg attcatggat agggctcggg caatcgcgac
    22561 acgctgtttt tgcccaccag agagcatttg agggtaggca ttatgcttgt cggctaaccc
    22621 gactttttca agaagttcga tgccctgctt tcgagcctta tcttttttca tatgtagcac
    22681 ctgggttggc ccttccataa cgttttgtag gactgtcata tgggggaaaa ggttaaagtg
    22741 ttgaaaaacc atgccgacgt ttcgtcgaag catattgatg tcattttttt gacgatgtat
    22801 cactttgccg tctatagaga tgcgacttcc tttttctgct gtttcaagaa aatttataca
    22861 gcgtagcaaa gtacttttgc cagaaccact tggtccgatt attacaacga cctcgccttc
    22921 gtcgacctta agatcgacac ccttaagaac cttaagcttg ccaaaatttt ttttgatatt
    22981 ttcgagtaaa atcatccgtt gctccgttta acttgttttg aagtggactt ccagtcgatg
    23041 tatcagatac gtgaaaagtg aagtaagaat cagataccaa atcgccacaa cgattagcat
    23101 ttccatcaga ataaaatggg tgttaccaat ttgccttgct cttaaaagta gttctgaaat
    23161 ggttattgtg cttgccaaag atgaatcttt aagtgcgatt atgaattggt taccaagaga
    23221 aggtatcgcc cttttgaatg cttggggcat tattattctt tccatcgctt ttgttttgct
    23281 catgcctagt gaacgggcgg cttccatttg ccctttgtcg attgactgga ttgctcctct
    23341 gaagatttca gcaatatagg ctccgttatg aataccgagc gcgattacgg cagatggaaa
    23401 agaacgtata gtgataattg acgtgatgcc gtaatagatt ataaaaagtt gcaaaagcaa
    23461 gggggttccg cgaataagga atatatatgc tttcgcagga tatctcataa acttgatttc
    23521 agaaacacgc attaaaccaa aaagaagacc aataagtaag ccaaaaagta ttcctgccga
    23581 tgttagctgt agggttatca gaccagcttt ccagaaggta ggaaaacgag tgatgatggc
    23641 tttaagactt tcgcccatag aaaatagaga tggattgata ttaacaaccc agtctataaa
    23701 tgatgacgat aataaaatat ttgtcgataa aaattccatt atgttttccg atcatcctgt
    23761 tgtttttagc agaaatcatc tgtaatgatt acaggtgatt tctgctcgtg gcatgtgatt
    23821 acttgacagt gatatcttca ccatcaaacc atttagtgct tattgacttg agtgtgccgt
    23881 ctttacgcat gtcatcaaga atcttgttaa tttcgtcttt aaggggactg tccttttgaa
    23941 gtgctattgc gcactcttct gttcttaata gtgaaccggc gagtgtcaga tcagatccgc
    24001 ctttgatttt ttttattgca ttaaggccaa caacacggtc tgtgataaca gcgtccacac
    24061 gtccatttat aagctccata agtgtctgat tgtcatcttc gtaaagattg atttttactc
    24121 ccagtttttc ggcatcttgc gcgaaagttg tcgcggtgac aagacccaca gtatgtttct
    24181 tgttaagctt gtcggggccg gttattgaag aatctttttt tgcaataatt tgcgctcctg
    24241 agtagtagta gggagtcgag aagtctacaa ctttgagtct ctcttcggta attgccattg
    24301 atccgagtat gccttcgtag cggccagctc tcagcccttc gatgattcca tcccatgatg
    24361 ttgtaacata aaccatctct ttgcccatgc gtttcgcgat ctcgtgagcg acgtccacat
    24421 caaaaccgat aacttcattg tcttcactca taaagttaaa gggagggtag ccgcctgatc
    24481 ccgcaaaacg gattacgttt tcatcttttt ttgcgcatga tgcgcttaat acgatgatta
    24541 aagttagtat taaaagttta agtttcatta gagtctcctt tatcttatat ttataaggtc
    24601 ataattataa ttgcttttgc tttgttatac ttgcgctgta taaatataaa tataatatct
    24661 gtaataagac agtgccaggt atagattgct atccgtgata atgcttggta aaccatgtaa
    24721 gcgatatttt gtttagcaaa gtcatttaag tgatatttaa aaccaaacaa ttgaccgcaa
    24781 acagtgtagt gaaatcatta tgtaagattt agaagttatg gttcgaagag tgacacgtaa
    24841 aagatcatac gtgtgtcacg gtttaaataa gatgttttaa aaagatttga aagctgtgat
    24901 attgacatta tgtaatttat tgaacttttt aaagttttca tttcttgata attgaggttt
    24961 gggaaaagaa ggtcaaggat ttttttatta taacacaacc ctatactctg aaaattaatc
    25021 agataaactt gttaaaggga catccggtat atttaaccgg atgtttgttg tagcaaataa
    25081 gagtagaact aatcagagat tattattgtc ctgctgttgc atcgattcga tctgtttttc
    25141 aagaagatct ctctgctgtc ttagctcttc aagtgttggt tcgtatggtc gttcgtatgc
    25201 tccaaagaat cttgctccga aaccgaatcc tgcacctcga cggccaaatc ccataccgcg
    25261 tccataaccg taaccagcgg ctctcccctg accacatgga cctaatccgc gtcccgtcat
    25321 tggaccgttt ccaaaagggc ctgttccatt aaatcctggc ataattgttt acctcctgtt
    25381 atataataat tttttacagc taagctgtaa aaactgaaac ttgcataatc gttagttcgg
    25441 tcgccctttt tagcctcggc cgcctctgtg tcttcgaccg ccacggacaa ctctttgcgc
    25501 gtattggccg ttcaagtttt gcaatttgtc agagctgcag tttggacaaa cagttaacga
    25561 tacctcattt attggagcct gataggtgtt atagcatgcg aggcatctga ttctgttgtt
    25621 ttcgaaatcg aactcaccac cttcgattaa gagctcgcaa ccgtcaataa ttgcttttgc
    25681 tattttgttg cgggcatttt gaagcatacg agtaaatact gatctggaga tacccagttt
    25741 ggatgaggca tcctgatgct ctagattatt ataatcggca aggcgcacgc actcgtactc
    25801 ttcgacagta ataactattg tatttatata ccgcgtagga ataccctgtg gcttaaaact
    25861 tttgcaatag ggcggacctt caattgtcct agtttttctt ggtcttgaca ttttgcacct
    25921 atgttcatta ctaaatataa gttacgcacc tatgtgcgta atgtcaactt tttttttatt
    25981 acatagtcca aacaattgcg caatcttcaa gcgcgattgt ttggacggtt tcggg
//
LOCUS       DAOTGZ010000012        25468 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_3064
            NODE_2253_length_25468_cov_5.034785, whole genome shotgun sequence.
ACCESSION   DAOTGZ010000012 DAOTGZ010000000
VERSION     DAOTGZ010000012.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868088
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 25468)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 25468)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:22:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,785
            CDSs (total)                      :: 2,751
            Genes (coding)                    :: 2,732
            CDSs (with protein)               :: 2,732
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 12 of 19
            Pseudo Genes (internal stop)      :: 5 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..25468
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2253_length_25468_cov_5.034785"
                     /isolate="AMR_MDS_3064"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..316
                     /locus_tag="P5123_01820"
     CDS             <1..316
                     /locus_tag="P5123_01820"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRX15032.1"
                     /translation="LGFIHDSSPCKTELRSETIMRAVILSTKYKDDYRSYHFYWFITD
                     LILSIGAMIIGESLYYSNIVAQSGRVFPILLFMLFAICIIIISIIYVGSHIKELKKFF
                     RV"
     gene            709..2793
                     /locus_tag="P5123_01825"
     CDS             709..2793
                     /locus_tag="P5123_01825"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis protein CheA"
                     /protein_id="HRX15033.1"
                     /translation="MSDQAILEVFHNEAREIIENIETELVSLEESYDSEKVNTLFRYF
                     HTLKGSSGIAGLMEVSEFTHEIESLLDRVRSEEIQVTPSLIDTVLASVDLVKVMIFDE
                     DQADGLSSQKEKILTFIGKNSDEVTDSSRPLPQKRKLNYFRVKINFKDNVFEYGIDPL
                     MIIEDLVTVGTVIEEKIYRSRVPALDEINPEKCYLSWMYIIGTKHTKKKINEIFLFVF
                     DDNDIEITDITEDYREDITSDVNEEKKIGNILVQKGLITEDELLFALSNMKKEEKVGK
                     ALVRLKLVTEKDIEKALGVQVEVRKKIESTTIRVERAKLDGIMNLLGEIVIGQSSIVR
                     FADELDEDKAYALKNSLYGLNRSTRQFQEQIMAMRMIPVGPTFSQFKRFVRDTSRQIG
                     KEIRLEIMGKETELDKTVIEEITDPLKHMIRNSIDHGIESTEERIKLGKDPTGIITLN
                     AYHQEGCIFIEISDDGKGLDADELRSIAVSKGIIAKDKELTEDESYALIFKPGFSTAN
                     AVGDLSGRGVGMDVVKSNIDALRGSVQIITEKNRGTTFRIKLPLTLAIIDGMLIKVGK
                     SVYIVPLLSILESIRPRKEDVKTIKGQGEVVMVRNEYISLIRLYKYFDQVPAFENPWE
                     ALVVIVESNGEKLALMIDDLIGQQQIVIKSLDNTVTKDRSISGAAILGDGNVSLIIDI
                     HGLLSEISSLRG"
     gene            2795..3061
                     /locus_tag="P5123_01830"
     CDS             2795..3061
                     /locus_tag="P5123_01830"
                     /inference="COORDINATES: protein motif:HMM:NF013868.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043856 - anti-sigma factor antagonist
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="STAS domain-containing protein"
                     /protein_id="HRX15034.1"
                     /translation="MSELIMEQNSSVKTVSNIKKQLLQIIDSEKELVIDFKSITSIDL
                     AIAQLLISAVKYARKKSVAVKFKNVSDEIRNQLQLTGLIKRNKE"
     gene            3065..3436
                     /locus_tag="P5123_01835"
     CDS             3065..3436
                     /locus_tag="P5123_01835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015923976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HRX15035.1"
                     /translation="MKNIIVIDDSPTIRTSVQFALKQLGHNIRQAENGLKGLEVVDEV
                     MDQKEDLALCVVDINMPEMDGITFVKEFRKKDKFTPVLVLTTESEEDKMKEGKAAGAS
                     GWMVKPFKPNDLIGVVQKLIR"
     gene            3447..3953
                     /locus_tag="P5123_01840"
     CDS             3447..3953
                     /locus_tag="P5123_01840"
                     /inference="COORDINATES: protein motif:HMM:NF013731.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis protein CheW"
                     /protein_id="HRX15036.1"
                     /translation="MEKIDRKKTVAEQIDDEANQYVTFLISGESYGVEVLCVKEILGM
                     TDVTPVPNTLSFMKGVINLRGEVVPVVDMRIKFGMSEKEYDAFTVIIIVEVKRRLIGM
                     IVDAVSDVADIPIDSIQNTPHFTTRIETDFIKGIGQLAEQLIIILDVDKILTSDEFQK
                     IDQEDVSE"
     gene            3953..4528
                     /locus_tag="P5123_01845"
     CDS             3953..4528
                     /locus_tag="P5123_01845"
                     /inference="COORDINATES: protein motif:HMM:NF015908.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis protein CheD"
                     /protein_id="HRX15037.1"
                     /translation="MKQLIDSNYRMPHYSIDPGEFMVSGERTLLESVAGKTLVVALYH
                     EKLKMGGLANFIVPGNIESGSIAESTVCETGIGYIEFLLADIVKSGGRRDELVAKIFG
                     ASCKRSDHDFCKTNIQFVHEYFENEKIPVIAEDLGREYYRRVLFVPVTGELYSKRILS
                     VPEMQLIYKREKDYIDREINGLQTETEFIMF"
     gene            4596..5423
                     /locus_tag="P5123_01850"
     CDS             4596..5423
                     /locus_tag="P5123_01850"
                     /inference="COORDINATES: protein motif:HMM:NF013867.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008983 - protein-glutamate
                     O-methyltransferase activity [Evidence IEA];
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein-glutamate O-methyltransferase CheR"
                     /protein_id="HRX15038.1"
                     /translation="MSPLPILDKLTDDEFAFFKAIVYREAGIKLSDMKVALLQSRIMR
                     RMRALQISTFRDYRNYLNDYYQDEIVDFINAVTTNKTEFLRENKHFDFMLSTALPNFE
                     KSNREEIRIWSAGCSTGEEPYSIAVTCCEYFNKNKIPVKILATDIDTQVLIQGYTGIY
                     PKNTIDVFTPTIQQRYFNAINTEKGVQYQIVPEVKKMITFKRLNLLDNTYPMRKKFDI
                     IFCRNVIIYFDKDTQRVLFEKMYNYIADDGFLFIGHSENLSGVNSNFKSVGHTIYRK"
     gene            5440..5940
                     /locus_tag="P5123_01855"
     CDS             5440..5940
                     /locus_tag="P5123_01855"
                     /inference="COORDINATES: protein motif:HMM:NF015908.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis protein CheD"
                     /protein_id="HRX15039.1"
                     /translation="MLSEYKSKLNRTVVKIFAGEYYIGCNSEIIKTTIGGCIAVCIWC
                     ANGSYGGMNHYMLPYKMNRSDYFPEGHTLYGINAVRELVDNLRRVGDKISDYEAAIIG
                     GSNIDLNDRSINNINMARLLLEEMDIRVIREDTGGSCIRKVSFDTYSGDIVIDKIDKT
                     SQLVFV"
     gene            5977..7020
                     /locus_tag="P5123_01860"
     CDS             5977..7020
                     /locus_tag="P5123_01860"
                     /EC_number="3.1.1.61"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004091451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0008984 - protein-glutamate methylesterase
                     activity [Evidence IEA];
                     GO_function: GO:0050568 - protein-glutamine glutaminase
                     activity [Evidence IEA];
                     GO_process: GO:0006482 - protein demethylation [Evidence
                     IEA];
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA];
                     GO_process: GO:0018277 - protein deamination [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis response regulator protein-glutamate
                     methylesterase"
                     /protein_id="HRX15040.1"
                     /translation="MADIRVLCVDDSALIRKLMNERLGSVDGIDVIATAPDPFIAAEK
                     IKTLNPDVVTLDIEMPRMDGLTFLEKLMKIKPLPVIMASSLTDKGAEQTIKAIKSGAF
                     DFVLKPQPGSEEHSVESFIMELAQKIKSAAKESVRKQRFNGIAKAKSVTQITGDCEAT
                     KRIIAIGASTGGTEVIATILTALPEKTPGIVITQHMPPKFTRAFAERIDSMAKINVKE
                     AEDGDVLKNSTAYIAPGGTQMSVRHIKDCFRIIVNDDPPLNRHKPSVDVLFNSVAECN
                     AKDTIGIILTGMGADGADGMKKMHDSGCYTIAQDEKSSVIFGMPNEAIKRGGVDKVEN
                     IEGIIQLLTKTIS"
     gene            complement(7024..7332)
                     /locus_tag="P5123_01865"
     CDS             complement(7024..7332)
                     /locus_tag="P5123_01865"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRX15041.1"
                     /translation="MIKVSGSTIIVLNEKATLEDKIDLENTIDALIAEGFNKIYLDLG
                     QTLHLPSELMGLLMWKKKVLNEGNIDIVISRISSTLKTVFETAHLIDFFEINNETLVV
                     "
     gene            complement(7572..9710)
                     /locus_tag="P5123_01870"
     CDS             complement(7572..9710)
                     /locus_tag="P5123_01870"
                     /inference="COORDINATES: protein motif:HMM:NF015983.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HRX15042.1"
                     /translation="MKDNILLVTPPLLQLNTPYPALLYLMGYLRQKGYTCTHYDLNIE
                     CAHSIFNSEFISKMYEVAISKKLEQPFHDFYENRERYISTIDPVIKFLTGANRTYAHR
                     IISRDLLPEGPHFDKVENSEIFSIFDTLDKGRYLATLYIEDIALAIRCCLDKGFEIVK
                     YKESTALGIPFFKTIQNELIKKPSQIEKTYLDLLRKKIVKSKPTIIGFTIPFPGTLFS
                     VLRCSQMIKNEFPDIIICAGGGFVNTELRYITSTDFFKYIDYLFFDDGEIPFEKLLEY
                     RTGKIDKTEIHNCLSLQGNSIANTVFKKSPISKNIMFPDYTDTDPSKYISMFDSENPM
                     MRLWNDGFWNKLTIAHGCYWSRCTFCDTSLDYINNFHPLHIEDIIARIEKCIADTGET
                     GFHFTDEAAPPALLKKIAIELLKRNISITYWTNVRFDTCYTADLAKLLAMSGCIALAG
                     GLEVASPRILSLIDKGVTVNQIAKATYNLSSAGILVHAYLMYGYPGQTAQETVDSLET
                     VRQLFANGCIDSAYWHKFTLTCHSRVFSNPEKYGIKIKKHDHDFCMNDIAFNEINKTN
                     HDHFTIPLNRALYNYMKMRELDKPVQYWFGEKTAPSSIKSDFIAKSLNNSAQTVYKSI
                     FIARKPEICCDSDCTTISFYTYGESYEINVSKKIGSDIEKFYNSHDLYMELAFDEAIQ
                     ILAPILKTDKNNIFFEKTFIFLREFSLFVL"
     gene            complement(9713..10399)
                     /gene="recO"
                     /locus_tag="P5123_01875"
     CDS             complement(9713..10399)
                     /gene="recO"
                     /locus_tag="P5123_01875"
                     /inference="COORDINATES: protein motif:HMM:TIGR00613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RecO"
                     /protein_id="HRX15043.1"
                     /translation="MTITSTEGVVLSSKISSEADIIASVFTETEGLTRFIIKGLKKSK
                     KRDISASDPGTLLNISFYKKTGSSIYYANNFSVIRHNIGNSITYENSMNAFLILELIM
                     KSSAPHMPHLYTYLMLKKAIESVCVTQFPFHLSLAFFCHLIKQQGILPDPLSFPVIKD
                     RNRSFKMNINDNGFVFLKTALTNKFDNIACSLFNEKDIKQTLYTLIIYFENYYHIEIL
                     SKSTLFNEKV"
     gene            complement(10404..11240)
                     /locus_tag="P5123_01880"
     CDS             complement(10404..11240)
                     /locus_tag="P5123_01880"
                     /inference="COORDINATES: protein motif:HMM:NF015436.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin family protein"
                     /protein_id="HRX15044.1"
                     /translation="MESDTEPGSTISPFLSFFKRSPRKQRGKEREILDFVNNGLEESK
                     KQMIQGIMKLSELCAREIMIPRVDIVSIRCNTSLKEIITIASDAGHSRIPVYDSTIDE
                     ITGILYVKNLLTYIIDKRRKFDLKRELHKPFFIPETMPLDELLVDFKKKRQHLAIVVD
                     EYGGVAGLVTMEDILEEIVGDINDEFDEHEAPEFVKLTKNSFDVDSRMSITDLNGEMG
                     VSLPTDEFDTIGGLVFDLFGKIPEKNDSIEYGSLRFEVKEIEGTRINRLTIRKTKSRK
                     ND"
     gene            complement(11285..11833)
                     /locus_tag="P5123_01885"
     CDS             complement(11285..11833)
                     /locus_tag="P5123_01885"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRX15045.1"
                     /translation="MAKAMSLLKAYKTNQIPMEGGFIISSFFTGSTIYTIYELTAFKN
                     VKDIFQGPDGLTFKTDGNRCHILVEPPTYSKNYVEPVHREDGASIPYRFDECHYHAGK
                     RQEKIYISKEPHMLYSSFTILNAMGDNYCFIFFPTKDVYMAIRKFMADSLYNDCGLRK
                     EDCRIVTEKFLETLHKFTVWSS"
     gene            12240..13001
                     /locus_tag="P5123_01890"
     CDS             12240..13001
                     /locus_tag="P5123_01890"
                     /inference="COORDINATES: protein motif:HMM:NF024206.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ankyrin repeat domain-containing protein"
                     /protein_id="HRX15046.1"
                     /translation="MYKMMQVLVVVLVSLFVVSCASGDKRPVNMELTNAANTGDLDKI
                     KKMVEEDGADVNSIDRKGYSTLHHASNKGYLDIVNYLNEKSVNVKTEDSGKAHPLHHA
                     AYMGHIDIVRQLVESGSDVNSMTEKGFTPVERASYRGHIDVVKYLVAKGADESIATVS
                     GDTPLHMAAFAGHVEIVKFFATRDKFDVNVVETRGYTALHYAAFKNRQDVVAVLLENG
                     ADATIASADGITALQLAERTKNAALISMIKSKIAE"
     gene            13054..13989
                     /locus_tag="P5123_01895"
     CDS             13054..13989
                     /locus_tag="P5123_01895"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRX15047.1"
                     /translation="MIYYKSSDFTIDSLINAKKIRCFVIFSAFGEREADLLCSKISLI
                     KNECNALVDKIIVSHRRIGNNEDKTEIKLKDEFDDVCLLNCNSYSVPDMRDEAGKGAD
                     MRRALYFCYKVLKANSHDCIVFLDADVDTELFRSEYFYALAGPIINAEAQFCKASFFR
                     KSGRVKKFTAQPLFSVINHPMLNGLSQFAYPLSGEVAGSFEFFASAGFAQRYGVETAL
                     LIDAVMQKIKMADVNLGNYDHIHGSDEDICRMSFGVIRTYLRKLEDYGVISLNENSAI
                     SNDFKRTYIDENLEMRSESDSLNEIISEPLQKIIS"
     gene            complement(13986..15062)
                     /locus_tag="P5123_01900"
     CDS             complement(13986..15062)
                     /locus_tag="P5123_01900"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRX15048.1"
                     /translation="MQYLKHTNRRFRLSNYLKAIIILLFIIHNPVLLAQKMSVGFVKY
                     VDISSKSRGSYSNYLANLLSARLSEDPTFSVKFIKQELTLEDIQSRNLAAFTTLSRNN
                     DCMYIVAGTYSVNHLNGELIYNSLIFNSQTKKFTLFETLQSEPGARLVSDTQKASLKI
                     HSNILSDYKVSSGFKDNTDYSKFCGLILGLNIGYTGFLTEWKDNYDNTTVLMPYLGYE
                     FQTSSGKTFLSVKADAIFFDSVDDIYDSDEDHYKNFSLVQAYGSSLDIFYTYGSYFRA
                     GVNAGGGYCIQTNYSESDGGSEEEEINENSSSDNKSGYLKGGLFIEAVPSALKITLGV
                     SFFHSFHDESIDSFIYYIGAGIRF"
     gene            complement(15073..16236)
                     /gene="argJ"
                     /locus_tag="P5123_01905"
     CDS             complement(15073..16236)
                     /gene="argJ"
                     /locus_tag="P5123_01905"
                     /EC_number="2.3.1.1"
                     /EC_number="2.3.1.35"
                     /inference="COORDINATES: protein motif:HMM:TIGR00120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004358 - glutamate N-acetyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006526 - arginine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional glutamate
                     N-acetyltransferase/amino-acid acetyltransferase ArgJ"
                     /protein_id="HRX15049.1"
                     /translation="MSNKIFSFSAVKSGIKYSDKYDFALIYSNAPCNCAATFTTNKVC
                     AAPVIISKERMANPIQAIVVNSSNANACTGEMGLENARSITSTIGSTLNIAPESILAA
                     STGVIGVQLPAQKMIDSIPDLTSNLSENNQNLFAQAIMTTDTVPKYVKKTFIANTKTY
                     TIEGFAKGSGMIAPNMATLLSFIVTDAPVEKNLLRDIFHDTIRKTYNCITIDGDMSTN
                     DSAFILSPLNDNYLDENGSNIFKDTLYQLLEELALILVKDGEGATRCVKINVKNALNN
                     DEARICACSVAESLLVKTAIFGKDPNWGRIACAAGYSGAQLDQNILSIYFNDIPLLLS
                     GTPVGYDKDKLMEIMNSESYEINIDIGSGNGSWSYWTSDISYDYVKINSEYTT"
     gene            16695..17027
                     /locus_tag="P5123_01910"
     CDS             16695..17027
                     /locus_tag="P5123_01910"
                     /inference="COORDINATES: protein motif:HMM:TIGR00377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043856 - anti-sigma factor antagonist
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="STAS domain-containing protein"
                     /protein_id="HRX15050.1"
                     /translation="MDLSTEEYDKLTIVKVSGEFHIGNVNKFEELWHTLIEKKPPVIA
                     IDCSNLEYIDSSAIGTLVKFLNNAMSKKIKLIFFDLNKAIQNIFKTARLDNFFTITTR
                     EQLEREYL"
     gene            17172..18242
                     /locus_tag="P5123_01915"
     CDS             17172..18242
                     /locus_tag="P5123_01915"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRX15051.1"
                     /translation="MKRYICFTAVSLLLMISCQRWNVNELKKNILFEIPAGTEPGEVN
                     LKFNNEGYVDLTFDVRVSNGKVFCSDNIAKRLQIFNLEGQPLMAIGDMEDGQVNNLIM
                     RNFSFGVIGNVISDQTGNVFVQNTILPGDRDGQKVEVDSATGMSPSYILVFNNDGRIM
                     YSLGQNGTADVPFYSIYNMFTDERSRLFVISKSYDNWSVFRYDGKERDFALHLDKNYF
                     KEKEKNENLAGRVENIIIFKNGEKLLISVAYYDNVRFKYRKIYEYKIIENRLGGAVIE
                     LPDPRNELFSLLEDKYIILWDVEKRDIRFTIWSLQENIINNLLIQMQGPRPFYEKVLV
                     DEMGRLYTMIVFDNSIEIKEWK"
     gene            18246..19778
                     /locus_tag="P5123_01920"
     CDS             18246..19778
                     /locus_tag="P5123_01920"
                     /inference="COORDINATES: protein motif:HMM:TIGR00196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0052855 - ADP-dependent NAD(P)H-hydrate
                     dehydratase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-hydrate dehydratase"
                     /protein_id="HRX15052.1"
                     /translation="MRLLDAAQMRGLDQTAIIKYNTPARDLMNCAALRAVDVVKSIIP
                     DNSRCAVICGNGNNGGDGFAVARLLNNANYRVSVFFDNDKRKSLSDESGLNHDLVSEL
                     VKPEFFPDNFDSFSVIIDAYLGIGIKGKLRENAKTLVEAVNRSGATVISLDIPSGLPT
                     EGYIPSNCVAIKADYTVTFGFPKVSMFSRRGREYCGTVITADIGLPSHEIFNNKPACY
                     IYEKKNFSDSSLLYQNDALYKTRAGCVTVFGGFRGYEGAAILSVKALSECGNGMTYLA
                     IDSDSDLSGREIPPEVMLHRLDADYKILCDYLLERSKTVVCGPGLGLQVKAKSLLSHI
                     LLRAAEGKIENLILDGDALTIVADTDINLVRKAGKACRLFLTPHSMEAARLSNVDVDE
                     IDGDSVGSAIEISKKYDAYVVLKGPATVTAYNDEVIINTSGNPLLATGGSGDVLTGII
                     ASFIRDDESIIDSVSSAVFLHGYCADYYYGKYRITSYKASDTINMIRPALKSLLESID
                     DH"
     gene            complement(19749..20630)
                     /locus_tag="P5123_01925"
     CDS             complement(19749..20630)
                     /locus_tag="P5123_01925"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024880.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HRX15053.1"
                     /translation="MKFDPLLKKCPLCDSTSISLKYNIIQSHSFNVYECQSCLFIFMN
                     PAFSDTFIASLYSDDYYSGKADYSYIDERDNHKFNSYVWKARIKKIHKYKKSGTFLDI
                     GCSFGGLLSSAQKYYDVYGVEISDFASKYCESINMKVHKGTLNDAPFKENSFDVITMI
                     ELIEHIKDPVNFIKKTASILKPGGLCVIQTADMGAFQAVSQAQNYHYFLPGHLSYFTA
                     CNLSSLLTKSGFTNIKIYRPVDFGLIPKLRKSRGQFKNFKDYKRWLTIAKYHFLGYFK
                     KKGRPMTSSMVIYAFKK"
     gene            complement(20679..21323)
                     /locus_tag="P5123_01930"
     CDS             complement(20679..21323)
                     /locus_tag="P5123_01930"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024880.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HRX15054.1"
                     /translation="MIILSDSSWNTHYSRERSLLGYPDENLVRLITSYKNSNDIDHKL
                     AIDIGAGSGRHVKLLKDFGFKNIFATDITEKAALINSNIAVPAIRCDTSKSPFKDNSF
                     DLAICWGSLHYDTQEGVTMQISEIERILKPGGQLFGTFRKADDNYLVREKKLSDNCWL
                     ISVNDLESCPVTFADENYLKILFKNFSFFEFGYMQRTRLNDLKIISHYFYKAIL"
     gene            complement(21336..22535)
                     /locus_tag="P5123_01935"
     CDS             complement(21336..22535)
                     /locus_tag="P5123_01935"
                     /inference="COORDINATES: protein motif:HMM:NF024925.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-grasp domain-containing protein"
                     /protein_id="HRX15055.1"
                     /translation="MFFFKRKRKPQKLFVSIAAGANQIPLIKAAREMGYRVISVDQDT
                     AAPGFIFSDIRIQESIKNTNEIYQKLRELLLDGKISAILTRSFAEAVFSTAELCDKLS
                     IPFIKPENVENFIDKQKMKTLFIDNNILTPDYSIYNPRRKKGYNFPAIVKPVVGHAKS
                     GVKLVNSLDDLIKFTNPYIKDKKQLIIEEYIDGDEIIVFGIVKKGTFYLYEITDKTTT
                     PPPYFVDIMHSSPSKHSSLYQEITEIAQKTATAFDINCSPLVLECKIRDNNIYVLEAV
                     AEFGGEYIPEVIISHTTGHNFFKAAIAAMTDDNFTPPSYKKNQNSSIVRYITGKKGRF
                     DTYTPLDTAFAKISLFKIFKNSNDPVNSPKTNHDRIGVVAACAKTKEIATERCEKAIN
                     KLNIQYK"
     gene            complement(22536..23474)
                     /locus_tag="P5123_01940"
     CDS             complement(22536..23474)
                     /locus_tag="P5123_01940"
                     /inference="COORDINATES: protein motif:HMM:NF012692.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HRX15056.1"
                     /translation="MHIGFDIGGTNIKAVITDKEGKVAASLKHPTGSNREEILNNIKL
                     VVDGLLDQTNSSLEELGPVGIGIPGSVDSEKGIILISPNIPSINDCNFASLVEEKIGK
                     KTYILNDATAALLGEWWQGNGKNYSNWIMVTLGTGIGGGAIVDGRLLEGRDGFAAEFG
                     HISIDLNGIPCSCGNRGCFERYASASSIAKLATEQISDYPDSRLNGETITAELLYSFA
                     KKGDIFAKHMMSQCARYLAFGVNSLLNIFNPEAVIFAGGLSHASDLLFPLMQKELDNI
                     GFKGIIEDIHFHTVLHEDQGPSLGAIKYVIEKTADN"
     gene            23916..24770
                     /locus_tag="P5123_01945"
     CDS             23916..24770
                     /locus_tag="P5123_01945"
                     /inference="COORDINATES: protein motif:HMM:NF014826.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PHP domain-containing protein"
                     /protein_id="HRX15057.1"
                     /translation="MKVIDLHLHSSCSDGIYSVKELAKKAYQEKISVLSLTDHDTVIG
                     ISEMKYYANKYSLSFISGIEFSCDFEDGDYHLLGYGIDHENSELIKEINYYSQERRNR
                     IDKIISKLNDNGIKISKDDVIKCGAAESPGKPHIARALIMKGYATNINDAFDHYLGKN
                     GMGNVPKSKISREKAYYLINKSGGTAVLAHPASLNRNVEQLKQIIEDDKKRGLYGIEL
                     FSQMHSDLQVEQYRKMADDCGLVVSGGSDFHGDKGETLGCYGNGRKIPFESVKPLLDR
                     FEIFVKKN"
     gene            complement(24732..>25468)
                     /locus_tag="P5123_01950"
     CDS             complement(24732..>25468)
                     /locus_tag="P5123_01950"
                     /inference="COORDINATES: protein motif:HMM:NF012797.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="RNA methyltransferase"
                     /protein_id="HRX15058.1"
                     /translation="LKKAVISGPKKSGIALVCGEKIITDNFAYFKNSIVCAVIYDGFS
                     EKNSGVNQIIDQLDKDSKLLLLKKSLYNDLDISGSKKLLLFVECPKNRYLETIDLTRP
                     SLIIPFQDPANTGSVMRSAAAFGITQIVVTAEAANPYNYKSIRTSGGAVFNLDIFDAP
                     SLIETADYFLRNGVKIISLDSSGKPVSDFSFPETFTVIPGIEGPGLPDKLLSHAVSIP
                     MTDKVESLNASTTVSILLYEYFKSIQ"
BASE COUNT         8105 a         4037 c         5088 g         8238 t
ORIGIN      
        1 attaggtttt attcatgatt cttcgccatg taaaacagag cttcgttctg aaacaataat
       61 gagagccgtt attctttcaa ctaaatacaa ggatgattat aggtcatacc atttttattg
      121 gtttataaca gatttaattc tcagtattgg agcaatgatt attggagaga gtctctatta
      181 ttcaaatatt gttgctcaat caggtcgtgt tttcccaata ctacttttta tgctttttgc
      241 gatatgtata ataatcattt caataatcta tgtaggctca catataaaag aactaaaaaa
      301 atttttcagg gtttaacctg ttttgcaagg gcaacatctc ttaaatttta ttagtaatcc
      361 taaggtttta atttatttct aaccatgagt atcgataaat atctataatt tctcatcctg
      421 ccgtgattac cccaaatatt ccttttaggg aacttaacca ttttgcaatt tatttatacc
      481 tgaatcgtta atctgatatt ttttatatgt taacaataaa aaatctgcaa aaacatggat
      541 tttgtttgaa ctcttattat attgttttga taagaagttt atcaaaggct atatgaaaac
      601 gtatcagtta acgttcttct tatactaata cttttgtatt agttattttt tattttccat
      661 aatgaaggga atatatgatt cccttaaatt gtatttgcgg gggtaaagat gtctgaccag
      721 gccattttag aggttttcca taatgaagct cgtgaaataa tagaaaacat agaaaccgag
      781 cttgtatccc tggaggagtc gtacgattct gaaaaagtaa ataccctgtt ccgttatttt
      841 catacactaa aaggaagttc cggaatagcc ggtttaatgg aagtatccga gtttactcat
      901 gagatagagt cacttcttga tcgtgtacgt tcggaagaga ttcaagtaac tccttcgctg
      961 attgatacag ttttagctag tgttgatctt gttaaggtaa tgatatttga tgaggatcaa
     1021 gcagacggtt tgtcttctca gaaagagaag attctaactt ttattggtaa aaatagcgac
     1081 gaagttacag atagctccag accattaccg caaaaaagaa agctcaatta ttttcgggta
     1141 aagatcaatt tcaaggataa cgtttttgaa tacggtattg atccactaat gattattgaa
     1201 gacctggtta ctgtaggtac agttatagaa gaaaagatat atcgatcacg ggttcctgct
     1261 cttgatgaga ttaatcccga aaaatgttat ttatcatgga tgtatataat cggaacaaag
     1321 cacacaaaaa agaagataaa tgagatattc cttttcgttt tcgatgataa cgatattgaa
     1381 ataactgata taaccgaaga ctacagagag gatatcacat ctgatgttaa tgaagagaaa
     1441 aagatcggta atattctcgt tcaaaagggc ttaatcaccg aagacgaact tctatttgct
     1501 ctttccaata tgaaaaaaga ggaaaaggtc gggaaagcgc ttgtaaggtt aaaacttgta
     1561 accgaaaaag atatcgaaaa agctcttggt gtacaggtcg aagtccgcaa aaaaattgag
     1621 tctacaacga tacgggtcga gcgggcgaaa cttgacggaa tcatgaattt actaggagag
     1681 attgttattg gtcagtcatc aattgtaagg tttgcagatg aactggatga agacaaggcc
     1741 tatgcgttaa aaaattcttt atatggtcta aatcgatcta caagacagtt tcaagaacag
     1801 ataatggcta tgcgtatgat tccggtaggt ccaactttta gtcaattcaa aagatttgtt
     1861 cgtgatactt cgagacaaat cggcaaagag attcgtttgg agataatggg aaaagagaca
     1921 gaacttgaca agactgttat cgaagagatt acagatcctc ttaagcacat gattagaaac
     1981 tccattgatc atggaataga gtcaacagaa gagcgtatca agcttgggaa agaccctaca
     2041 ggtattataa cccttaacgc ctatcatcag gaaggatgta tctttattga aatatctgat
     2101 gatggcaaag ggttagatgc tgacgagctt aggtcaattg ccgtttcaaa aggtattatt
     2161 gcaaaggata aagagctcac agaagatgag tcatacgcac tgatttttaa accaggattt
     2221 tcaactgcta acgcggtggg tgatttatca ggacgcggag tgggtatgga tgttgtaaaa
     2281 agcaatattg atgccttgcg tggatcagta cagatcatta ccgaaaaaaa tcgaggaaca
     2341 acttttcgta taaaacttcc gcttacttta gcaataattg atggaatgct tatcaaggtc
     2401 ggaaagagtg tttatatagt gcctctactt tccatcttgg aatcgataag acctcgcaag
     2461 gaggatgtaa aaactatcaa ggggcagggc gaagttgtaa tggttcgaaa cgagtatatt
     2521 tctttgatac gattatataa gtatttcgac caggttcctg cttttgaaaa tccatgggag
     2581 gctttggttg ttattgttga gtcaaacgga gaaaagctgg ctttgatgat tgatgattta
     2641 attggtcagc aacagatcgt aatcaagagt ttagataata cagtaacgaa agatcgttca
     2701 atatccggag cggcaatact gggagacggt aatgtctctt taataattga tatacatggt
     2761 cttttaagtg agatcagttc tttgcggggt taatatgtcc gaacttatta tggaacaaaa
     2821 cagtagtgta aaaactgttt caaacattaa aaagcagctt ttacaaataa ttgattctga
     2881 aaaggagtta gttatcgatt ttaaaagtat caccagtata gatttggcta ttgctcagtt
     2941 gttaatatca gcagtaaaat atgccagaaa aaaaagtgtg gccgttaagt ttaaaaatgt
     3001 atcggacgaa atacgaaatc aacttcagct tactggtcta atcaaacgga acaaggagta
     3061 atttatgaaa aatatcatag tcattgatga ttcaccgact atacgcacaa gcgttcaatt
     3121 tgctcttaaa caacttggac ataatatacg tcaggccgaa aatggtttaa aaggtctgga
     3181 ggttgtcgat gaagtgatgg atcaaaagga agatttggcg ctttgtgttg tagatataaa
     3241 tatgcctgaa atggatggta ttacattcgt taaggagttt aggaaaaagg acaagtttac
     3301 tccggttctt gtccttacaa ccgaatcaga agaagataaa atgaaagagg gtaaagctgc
     3361 tggagcatcg gggtggatgg ttaagccgtt taaacctaat gatcttatag gtgtagttca
     3421 gaagctaatt agataagggg tgcacaatgg aaaaaataga cagaaaaaaa acagtcgccg
     3481 aacagataga tgatgaagca aatcagtatg taacgtttct gatatccggt gaatcctatg
     3541 gagttgaggt gctttgtgtt aaagagattc tgggaatgac tgatgtgact cctgttccta
     3601 atactctttc ttttatgaaa ggagttataa atcttcgtgg agaggttgtg ccagttgttg
     3661 atatgagaat caaatttggg atgagcgaaa aagagtacga tgcatttaca gtaataatca
     3721 ttgtagaggt aaaaaggcga cttattggaa tgattgttga tgctgtatca gatgtcgctg
     3781 atattccgat cgattctata cagaatactc ctcattttac aacaagaata gagaccgact
     3841 ttataaaagg aattggtcag ttggcagaac agttgataat tattcttgat gtcgataaaa
     3901 ttctaactag tgacgagttt caaaaaattg atcaggaaga tgtttcggaa taatgaagca
     3961 gttaatagat tcaaactata gaatgccaca ttactcgata gatcccggcg agttcatggt
     4021 gtcaggtgag agaacactgc tggagtctgt agcagggaaa actttggttg ttgcgctgta
     4081 ccatgaaaaa ctgaagatgg gcgggttggc aaatttcatt gttcccggta atattgagtc
     4141 aggttcaatt gccgaaagta ctgtttgtga aactggaatt ggttatattg aatttttgct
     4201 tgctgatata gttaaaagcg gtgggcggcg tgatgagcta gtggcaaaaa tatttggcgc
     4261 ttcttgcaaa agaagcgatc atgatttttg caagacaaat attcagtttg tacatgagta
     4321 ttttgaaaac gaaaaaattc cggttattgc cgaagatctt ggtcgtgaat attatagacg
     4381 tgttcttttt gttccggtaa cgggagaatt atattcaaaa agaatactat ctgttccaga
     4441 gatgcagttg atatacaaaa gagagaagga ttatattgac cgtgaaatta acggcctgca
     4501 aacagagaca gaatttatca tgttttagca agcttatcag tataattttt ttataatcat
     4561 aattcttgcc ctgatttaat tttaaggaaa aaaacatgag cccattgcca atattagata
     4621 agctgactga tgatgaattt gcgtttttta aagctattgt ttaccgtgaa gctggtataa
     4681 aattatcaga tatgaaagtt gcgctactgc aatcacgaat tatgcgacgc atgagggctt
     4741 tacagatttc cacttttcgc gattatcgaa attatcttaa tgattattat caggatgaaa
     4801 ttgttgattt tataaacgca gtaacaacaa ataaaaccga gtttttacgt gaaaacaaac
     4861 atttcgattt tatgctttct acagctttgc caaattttga aaaaagcaat cgtgaagaga
     4921 tacgcatttg gagtgctggt tgttcaaccg gtgaagagcc ttattcaata gctgtaactt
     4981 gctgtgaata ttttaataaa aataaaattc cggtcaaaat tctggcaacc gatattgaca
     5041 cgcaggttct tatccaggga tatacaggaa tttacccaaa aaacacaatc gatgttttta
     5101 ctccgacgat tcagcaacgt tatttcaatg caattaacac agaaaagggc gtacaatatc
     5161 aaatcgtgcc tgaagtaaaa aagatgatta ctttcaaaag gctaaatttg ctggataata
     5221 cctatcccat gagaaaaaaa tttgatatca ttttttgcag aaatgttatt atctattttg
     5281 acaaagatac tcaaagggta ctttttgaaa aaatgtacaa ttacattgct gatgatggtt
     5341 ttctctttat agggcattca gaaaatttgt caggtgtaaa cagtaatttt aaaagtgtcg
     5401 ggcatacaat ataccgtaaa taggtcaata agatcaatta tgttaagcga atataaaagc
     5461 aaactaaacc gaacagttgt aaaaattttt gcaggtgagt attatatagg gtgtaactcc
     5521 gagataatta agactactat cggtggatgt attgctgtgt gtatatggtg cgcaaatggt
     5581 tcttatggag gtatgaacca ttatatgttg ccctataaaa tgaacagaag tgattatttt
     5641 ccagagggcc atactttata tggaataaat gccgtaagag agctagttga taatcttaga
     5701 agagttggtg ataaaattag tgattatgag gcagcaatta ttggcggttc aaacatcgat
     5761 ttaaatgata ggtctatcaa taatataaat atggcaaggc tgcttttgga agagatggat
     5821 attagagtta taagagaaga tactggtggt agctgtatca gaaaggtatc ttttgatact
     5881 tattctggag atatcgtaat agacaaaatt gataaaacca gtcagttagt gttcgtataa
     5941 tgttgaatgt aaataaatcc aagaaagagg ttgaagatgg ctgatatccg tgtactttgc
     6001 gttgatgatt cagcacttat tcgaaaactt atgaatgagc gtttaggttc tgttgacgga
     6061 attgatgtta ttgcaacagc acctgatcca tttatagccg cagaaaagat taaaacactt
     6121 aatcccgatg ttgtaacttt ggacattgag atgccaagaa tggatggtct gactttcctt
     6181 gaaaaactga tgaaaatcaa gcctttaccg gtcattatgg ccagttctct tacagacaaa
     6241 ggtgcagaac agacgataaa agcaattaaa tctggtgctt tcgattttgt attaaaacct
     6301 caaccaggat cagaagagca ttctgttgaa agtttcataa tggaattagc acaaaaaata
     6361 aagagtgcgg cgaaggaatc agttcgtaaa cagcgtttta acggaattgc taaagcaaaa
     6421 tctgttactc agattactgg agactgtgaa gcaaccaagc gaataattgc gattggtgca
     6481 tctacgggag gcacagaagt tatcgcaaca atactgaccg cacttcccga aaaaaccccg
     6541 ggtattgtta ttactcaaca tatgccaccc aagtttacaa gggcgttcgc ggagagaatt
     6601 gattcaatgg caaaaatcaa tgttaaagag gccgaggatg gtgacgtgct aaaaaattca
     6661 acagcttata ttgcccccgg tggtacacaa atgtctgtac gacatatcaa ggattgtttc
     6721 agaattattg ttaatgatga tccacctttg aacagacata aaccatcggt agatgtattg
     6781 ttcaattccg tggctgagtg taacgcgaaa gatacaatcg gaataatcct gactggtatg
     6841 ggagcagatg gtgccgatgg tatgaagaaa atgcacgatt caggatgtta tacaatagct
     6901 caagatgaaa aatcatcagt aatttttggt atgccaaacg aagccataaa aagaggtggt
     6961 gttgataagg tcgaaaatat tgagggaatt attcagttgc taaccaagac cataagttag
     7021 aatttaaaca actaaagttt cgttattgat ttcaaaaaaa tcaattaaat gcgctgtttc
     7081 aaatacagtt tttaaagtag aacttattct actaattaca atatcgatat taccttcgtt
     7141 aagaaccttt tttttccaca ttaataaacc cattaattcc gaaggcaaat gtagggtttg
     7201 acccagatct agatatatct tgttaaatcc ttcagcgatt aacgcgtcaa ttgtgttctc
     7261 aagatcaatt ttatcttcaa gcgtagcctt ttcatttaat actataattg tagatccgct
     7321 cactttaatc atatataacc tgtatgcatt tattgattac aattacatac attgtaaaaa
     7381 tgaggtaata gctgtcaatc ttaattgaga ctataggttt taaaagttac accctgtgct
     7441 ttatttacga tgaattatat ataacgttaa aagattttgt taaacgtgca acggtaaaaa
     7501 taattaagct gcaaaagtta gtgaaaatag agttacaaaa agggaatcaa gtaacatatt
     7561 taggaaaatc tttatagcac aaacagagag aattctctaa gaaaaataaa tgttttttca
     7621 aaaaaaatgt tattcttatc agtttttaaa atgggtgcaa ggatttgtat cgcttcatca
     7681 aatgctaatt ccatataaag gtcatgactg ttatagaatt tttctatgtc tgatccaatt
     7741 ttctttgata cattaatctc ataactttct ccatatgtgt aaaatgagat tgtggtgcaa
     7801 tctgaatcac agcaaatctc tggtttgcgt gcaataaaaa ttgatttata tacagtttga
     7861 gcactgttgt tcagagattt tgctataaag tcgcttttaa tggatgatgg agcagttttc
     7921 tccccaaacc agtattgtac cggtttgtcc agttctctca ttttcatata attatagagg
     7981 gctctattaa gtggaattgt aaaatgatca tggtttgttt tgttgatttc gttaaatgct
     8041 atgtcattca tacaaaaatc atggtcgtgt ttttttattt ttattccata tttctctgga
     8101 ttcgagaata cccgactgtg acaggttagc gtgaatttat gccagtacgc agaatctata
     8161 catccgttag caaagagttg tctgacagtt tcaagagaat ccaccgtctc ttgtgctgtt
     8221 tgccccggat aaccatacat caagtaagcg tgaacaagta tccctgctga tgacaagttg
     8281 tatgtagctt ttgcaatctg gttaacagtt actcctttat caataagtga caatattcta
     8341 ggtgaagcta cttcaaggcc acctgcaagg gcaatacatc ctgacatggc aagtagcttc
     8401 gcaagatcag cagtgtaaca ggtatcaaag cggacatttg tccagtatgt tatgctaata
     8461 ttgcgtttta aaagttcgat ggcaattttt ttaagaagtg ctgggggagc tgcttcatcc
     8521 gtaaaatgga aacctgtttc tccggtgtcg gcgatacatt tttcgattcg tgcgataata
     8581 tcttcaatgt gcaatggatg aaaattgttg atatagtcga gtgaagtatc gcaaaaggtg
     8641 catcttgacc agtaacatcc gtgggcaatt gtaagcttgt tccaaaatcc gtcgttccag
     8701 agacgcatca tggggttctc tgaatcaaac attgaaatat attttgatgg gtctgtgtcc
     8761 gtataatcgg gaaacatgat attctttgaa atgggacttt ttttaaaaac agtatttgct
     8821 atagaattgc cctgtaaact aagacagttg tgaatctcag ttttatcaat cttacctgtg
     8881 cgatattcaa gcagtttttc aaatggaatc tccccgtcat cgaaaaaaag ataatcaata
     8941 tatttaaaga agtctgttga agttatgtat cttagttcgg tatttacaaa tccgcctcca
     9001 gcacatatga ttatgtccgg aaattcattt ttgatcatct ggctgcaccg taaaactgaa
     9061 aaaagagtac caggaaaagg aattgtgaaa ccaattatag ttggcttact cttcactatt
     9121 ttttttctta aaagatccag atatgttttt tcaatttgtg aaggtttctt tattaactca
     9181 ttttgaatgg ttttgaaaaa tggaattcct aaagcggtag attccttata tttcacaatt
     9241 tcaaaacctt tatccagaca acatcttatt gcaagggcga tatcctctat atacagagtt
     9301 gccagatatc gtcctttgtc tagagtatca aaaatggaaa aaatttcaga attttcaact
     9361 ttatcaaagt gcgggccttc gggtaaaagg tctcttgaaa ttattctatg agcgtaggtt
     9421 ctgttggctc cggtaagaaa tttaattacg ggatcaattg ttgagatgta acgctctcgg
     9481 ttttcataaa aatcatgaaa tggttgttcg agtttcttac taatggcaac ttcgtacatt
     9541 ttggaaatga attcggaatt gaagatagag tgagcacatt cgatatttaa atcataatga
     9601 gtacaagtat aacctttttg acgtagatag cccatgagat acaagagagc cggataaggg
     9661 gtattaagtt gaagtagtgg gggggtaact aacagaatat tgtctttcat tattatactt
     9721 tttcgttaaa gagagtggat ttggataaaa tttctatatg gtaataattt tcaaaataga
     9781 taataagagt atagagcgtt tgtttgatat ctttttcatt gaaaagcgaa caagcaatat
     9841 tgtcaaattt atttgttaaa gctgttttta aaaaaacaaa accattatca ttaatattca
     9901 ttttaaaact acgattgcga tcttttataa ccggaaatga taaggggtca ggaagaatac
     9961 cttgttgttt tattaaatga caaaaaaaag ccaaagacag gtgaaacgga aactgggtta
    10021 cacaaacaga ttctattgct ttctttaaca taaggtacgt gtaaagatga ggcatatgtg
    10081 gtgctgaact tttcattatt agttcaagta taagaaaggc gttcattgag ttttcatagg
    10141 tgattgaatt accaatatta tgccttatta cagaaaaatt attagcataa tagatagatg
    10201 atcctgtttt tttgtaaaaa gatatattta gcagcgttcc tgggtctgat gcgcttatat
    10261 ctcttttttt tgatttttta agacctttga tgataaaccg agttagcccc tctgtttcag
    10321 taaaaacaga ggcgataata tctgcttcgc ttgaaatctt ggaagaaagt actactcctt
    10381 cggttgaagt tattgtcatt tatctagtca tttttacggg attttgtttt tcttattgtt
    10441 aacctgttaa ttcgtgtacc ttcgatctct ttgacttcga atctcagtga gccatattca
    10501 atagaatcgt ttttttcggg gattttaccg aaaagatcaa aaacaagacc gccgattgta
    10561 tcaaattcat ctgttggtag agaaacaccc atttcgccat taaggtcggt aattgacatt
    10621 cgcgaatcaa catcaaaaga atttttggtc agctttacaa attcaggcgc ctcgtgttcg
    10681 tcaaattcgt catttatatc accaacaatc tcttcaagaa tgtcttccat cgttacaagc
    10741 ccagcaactc cgccatattc atcaactaca atggctaaat gctgtctttt tttcttgaaa
    10801 tcgacaagta actcatctag gggcattgtt tcgggtatga aaaaaggctt atgaagttcc
    10861 ctttttaaat caaatttgcg tcgtttgtca ataatatagg tcaaaagatt ttttacgtaa
    10921 agaatacctg ttatttcatc aatagttgag tcataaacag gtattcggga atgcccagcg
    10981 tctgaggcta tggtaataat ttcctttaga gaggtattac atcttatcga aactatatcc
    11041 acacgaggta tcataatttc ccgtgcacaa agctccgaca gcttcatgat accttgaatc
    11101 atctgttttt tagattcttc tagcccgtta ttaacaaaat cgagtatttc cctctctttc
    11161 ccacgctgtt tccggggtga acgtttgaaa aaagacaaaa atggagaaat ggtactccca
    11221 ggttccgtgt cagattccat atcggtttaa aaaaactccc ttaataatca gttttaagca
    11281 acaattatga agaccatacg gtaaacttgt gtagagtttc aagaaatttt tctgttacta
    11341 ttctgcaatc ttccttacga agcccacaat cgttatataa tgaatctgcc ataaactttc
    11401 tgatggccat ataaacgtct ttggttggaa agaagataaa acagtagtta tctcccattg
    11461 cgtttagaat ggtgaaagat gaatagagca tatgaggttc tttggatata taaatctttt
    11521 cctgccgttt gccagcgtgg tagtggcact catcaaatct ataagggata gaggctccgt
    11581 cttcacggtg tacaggctca acataatttt tagagtatgt tggaggttct actagaatat
    11641 gacatctgtt accatcggtt ttaaaagtaa gcccgtcagg tccttgaaaa atatccttaa
    11701 catttttaaa cgctgttaac tcgtaaatag tgtatattgt agatcccgta aagaaagagg
    11761 agataataaa tccgccctcc ataggtatct gattagtttt gtaagccttt aatagggaca
    11821 ttgcttttgc catgacatca cactccagaa gtttgtcgat ttcgttgata ttgtgtaatt
    11881 gattagtaca tataacaata taaacctcgt ttaatatact atttatgtgt aattatatca
    11941 atgttttttt atctgaaaaa gctaaaaaga aatagtacta tttataaaaa agtgttggta
    12001 caaagctgaa aaggactcaa attgatataa aaagggcttg cattgatgat aataactctg
    12061 gtataatgtc aaaatgtctc aaggataatc ggttttacgc catccttgag agtttggaaa
    12121 gtttttttaa gaaagctgga tccggagcag taatagctct atcatggggg tctttcttaa
    12181 taaaaacaat gggggaatta cctctggaaa taataaatgt attttaaaag gagattgtta
    12241 tgtacaaaat gatgcaggtt cttgttgttg ttctggtctc attgtttgtt gtgtcctgtg
    12301 cttccggcga taaacgtcct gttaacatgg aacttaccaa tgccgctaat accggagatc
    12361 ttgataagat caaaaagatg gtagaagaag atggagcgga tgttaactcg atagaccgaa
    12421 agggatacag tactcttcat catgcttcca acaaaggata tcttgatatt gtcaattatc
    12481 taaacgaaaa atcggttaat gtaaagacag aggatagcgg taaagcgcat ccactacatc
    12541 atgccgcgta tatggggcat atagatatag tacgtcaact ggttgaatca ggttctgatg
    12601 ttaattcaat gaccgagaaa ggatttacac ctgttgaaag agcttcttat cgaggtcata
    12661 tcgacgtagt gaaatatctt gttgctaaag gtgcagatga gagtatcgct actgtttcgg
    12721 gagatacacc tttgcatatg gccgcttttg ccggacatgt agagatcgtt aagtttttcg
    12781 caactcgtga taaatttgac gtgaatgtag tcgaaactag aggatatacg gctttgcatt
    12841 atgcggcttt taaaaacagg caagatgttg ttgcagtttt attggaaaat ggcgccgatg
    12901 ctacaatagc aagtgctgac ggaattactg ctttgcaact tgcagagaga acaaaaaatg
    12961 ctgcattaat ctccatgatt aaatcaaaaa tagctgaata aatcaacaag attacaggtg
    13021 ggatatccgc ctgtaatctt ctccggatca caaatgatct attataaatc ttccgatttt
    13081 accatagaca gtcttattaa cgcaaaaaaa atcagatgtt ttgttatatt ttcggctttc
    13141 ggtgaaaggg aggccgattt gttgtgctca aaaataagct taatcaagaa tgaatgtaat
    13201 gccttggtcg ataagattat cgtatctcac cgacgtattg gtaataatga agataaaact
    13261 gagattaagt taaaggatga atttgatgat gtttgcctct taaattgtaa cagttattct
    13321 gttccggata tgagagacga ggcgggtaag ggggccgata tgcgacgcgc tctctatttt
    13381 tgttataagg ttcttaaagc aaatagtcat gattgtattg tttttcttga tgctgatgta
    13441 gacactgaat tatttcgcag tgaatatttt tatgctttag ctgggccaat aattaatgca
    13501 gaagctcagt tttgcaaagc ctcttttttt agaaagtctg gtcgtgtaaa gaagttcaca
    13561 gcacagccac ttttttcggt gattaatcat cctatgttaa atggtttatc acaattcgcg
    13621 taccctttgt ctggtgaggt tgcgggtagt tttgagtttt ttgcatctgc tggttttgct
    13681 cagcgttatg gagtcgaaac ggcattgcta atcgacgctg ttatgcaaaa aattaaaatg
    13741 gccgatgtta atctcggtaa ttatgaccac attcatggta gtgatgagga tatttgcaga
    13801 atgagttttg gggttataag aacttatcta agaaagcttg aggattatgg ggtaataagc
    13861 ctgaatgaaa attccgcaat ttcgaatgat tttaaaagaa cttatataga cgaaaatctg
    13921 gaaatgcgct cggagagtga ttcattaaac gaaattattt ccgaaccatt acaaaaaata
    13981 atcagttaaa accttattcc ggctcctata taatagataa aactgtctat actttcatca
    14041 tgaaaagagt gaaaaaatga taccccgagt gtaattttca aagcagaggg aacggcttca
    14101 ataaataatc cgcctttcaa atatccactt ttattgtcag aactgctatt ttcattgatt
    14161 tcctcttctt cacttccacc gtcagattct gaataattcg tctgtataca ataaccgcca
    14221 ccggcattaa ctccggctct gaaatatgat ccatatgtat aaaaaatgtc taatgaagag
    14281 ccgtatgctt gaacaagtga aaagttttta taatgatctt catctgagtc atatatgtcg
    14341 tcaactgagt caaagaaaat tgcatcggct ttgaccgaaa gaaaagtttt tccggaagat
    14401 gtctggaatt cataccccag atagggcatt aatacagttg tattgtcgta attatctttc
    14461 cattcggtta aaaaaccggt atagccaata tttaaaccca gaattagtcc gcaaaacttg
    14521 ctgtaatcgg tgttatcttt aaagcccgaa gaaactttat aatctgaaag aatatttgag
    14581 tgtattttca atgaagcttt ctgggtatcg cttacaaggc gggcgcctgg ttcagattgt
    14641 aaagtttcaa agagggtaaa ttttttagtt tgtgagttaa aaataagtga gttgtagatc
    14701 aattcaccgt taagatgatt taccgaatat gtgcctgcaa ctatatacat acaatcgtta
    14761 tttctagaca aagtggtaaa tgctgccaga ttgcggcttt gaatatcttc aagagtcagc
    14821 tcttgtttta taaattttac agaaaaagtg gggtcttcgg ataatcgtgc ggatagcagg
    14881 tttgccaggt agtttgagta agaacctcta ctttttgaac tgatatctac atactttaca
    14941 aagcctacac tcattttttg tgcaagcaga acaggattat gtataataaa aagtaaaatt
    15001 attatagctt ttagataatt ggataatctg aatcttcgat ttgtgtgttt taagtattgc
    15061 atatcctcta ccttaggttg tatactcact atttattttt acataatcat acgatatgtc
    15121 agatgtccaa tagctccagg atccattacc ggagccaata tcaatattta tctcgtatga
    15181 ttcactgttc ataatttcca ttaatttgtc tttgtcatac ccaacaggag taccactcaa
    15241 caagagagga atatcattga aatatatgga gagaatattc tgatccaatt gggctccgga
    15301 ataacctgct gcgcaagcta ttcttcccca attcggatcc ttgccaaaaa ttgctgtttt
    15361 tacaaggagt gattcggcaa ctgagcaggc acagatacgg gcttcgtcgt tattcaaggc
    15421 atttttgaca ttaattttta cacatcttgt tgcaccttct ccatctttaa ctagtatcag
    15481 ggcaagctct tcaagtaatt ggtaaagcgt gtctttgaaa atatttgagc cgttctcgtc
    15541 cagataattg tcgtttaaag gagaaagaat aaatgcacta tcgttggtgg acatgtcacc
    15601 atcaattgta atgcagttat aggtttttct tatcgtatca tgaaaaatgt cacgtaataa
    15661 atttttttca acaggggcat ccgttacaat aaatgacaat agagtagcca tgtttggggc
    15721 aatcatgcca gagcctttcg caaatccttc tattgtgtag gttttagtgt tggcaataaa
    15781 tgttttctta acatattttg gtacagtatc ggttgtcatg attgcttgag caaacagatt
    15841 ttgattgttt tctgaaaggt ttgaggttag atcaggtatt gaatcaatca ttttctgcgc
    15901 gggaagttgt accccgatta caccagtcga tgcagctaaa atggattccg gggcaatatt
    15961 taaggtcgat ccgattgtcg acgtgatact tcttgcgttc tcaagcccca tctctcctgt
    16021 acatgcattt gcgtttgatg aattgacgac tatagcctgt attgggttgg ccatacgctc
    16081 ctttgaaata atcacgggtg cggcacaaac tttgttggtt gtaaaggttg cagcacagtt
    16141 gcaaggggca ttgctgtaaa taagagcgaa atcatattta tcgctatatt tgatgccgct
    16201 tttgactgca gaaaaagaaa aaattttatt gctcatagag cctccgtgta gcaaggtaat
    16261 agtattaatg atttgaaggc agatctcgta tctgcaacgg ctaacatcat gtattcttac
    16321 acttatttaa gttaaattaa aaaaaatagc tagctttgtc actaattttt catctcacta
    16381 ttttgtgtaa gatctttttt tttgatatcg tgaaaaaagt aatcattaaa aagcgctatt
    16441 tgttagcgat tttcaagtca taaatataat aattgcttct ttattggttt tttgatatca
    16501 aattaaaaaa ttgttgatta tagtgagaga aaatttatat ttgatgtagt tgtacaaaaa
    16561 agtttttacg tatcaaaaac tgttcttcaa aagaatttgc tgaattattt accgattatt
    16621 gctgtttttg tggttatttt tgatgaataa tctgaatgac taataattga ttatgtttta
    16681 cgggagatta tgaaatggat cttagtactg aagagtatga taaacttaca atcgtcaagg
    16741 tttctggtga atttcatatc ggaaatgtga ataaatttga agagttgtgg catactctta
    16801 ttgaaaaaaa acctccagtg atagccattg attgttcaaa tcttgagtat attgactcat
    16861 cagcaattgg aacgctagtc aagtttttga ataatgcgat gagtaaaaaa attaaactga
    16921 ttttctttga tttgaataag gcgatacaga atatttttaa aaccgcacgt ctggataatt
    16981 ttttcacaat aaccactcgt gaacagttag agcgtgaata tttgtagcca taggtgttct
    17041 gacagttttt ttatcttgaa attaggtcat gttagaagtt aatgtgaaga atcttgtttc
    17101 ctgaggtatt gttacaatac ctcttatttt tattcaaaat gtataaagta ataccccttg
    17161 gagaaagaat tttgaagcga tatatctgtt ttacagctgt ttcactgtta ttgatgatct
    17221 cttgccaaag atggaatgtt aatgaactta agaaaaatat tctttttgaa ataccggctg
    17281 gaacagagcc tggagaggta aatctaaaat ttaataatga aggttatgtc gatcttacct
    17341 ttgatgtgcg agtaagtaac ggaaaggttt tttgctcgga taatatcgcg aagcgacttc
    17401 agatttttaa tctggagggg caacccttaa tggctattgg tgatatggag gatggccaag
    17461 taaataatct cattatgcgt aatttctcat ttggagtaat tggcaatgtg atctccgatc
    17521 aaactggaaa tgtttttgtt caaaacacca ttttacctgg agatagagat ggtcagaaag
    17581 tagaagtgga ttctgccact ggaatgtctc catcttatat tctcgtattt aataatgacg
    17641 gccgtatcat gtacagcctg gggcaaaatg gtacagcgga tgttcctttt tattccatct
    17701 ataatatgtt tacggatgag agatcgcgcc tgtttgttat atcaaagtcg tatgataact
    17761 ggtctgtatt tcgctacgat ggtaaggaac gtgatttcgc tcttcatcta gataaaaatt
    17821 attttaaaga aaaagaaaaa aatgaaaatc ttgccggtcg tgttgaaaac attattattt
    17881 tcaaaaatgg tgagaaactg cttatctctg tcgcttatta cgataatgta cgctttaagt
    17941 atcgaaagat atatgaatac aaaattatcg aaaaccggct gggaggtgct gttattgaac
    18001 tacctgaccc tcgaaacgaa cttttttctc ttcttgagga caaatatatt attctatggg
    18061 atgtagaaaa gagggatatt cgttttacta tatggagcct tcaagaaaat attataaata
    18121 atcttctcat ccagatgcag gggccaaggc ctttttatga aaaagttctt gtagatgaaa
    18181 tggggcgttt gtataccatg atcgtttttg acaatagtat agaaatcaaa gaatggaaat
    18241 agaatatgcg tcttcttgat gctgctcaaa tgcgtggtct ggatcaaact gcaataatta
    18301 agtacaacac tcctgcacgg gatctgatga attgtgcagc gttaagggcg gtagatgtag
    18361 taaaaagtat tattccagat aattcacgat gtgctgttat ttgtggtaat ggaaataatg
    18421 gaggagatgg gtttgccgtt gcccgtcttt tgaacaacgc taattatcgg gtttcagttt
    18481 tttttgataa tgacaagcgc aaatctttat cagatgaatc tggcttgaat catgatcttg
    18541 taagtgaact agtgaagcct gaattttttc ctgataattt tgactccttt tctgtaatta
    18601 ttgacgccta tcttggtatt ggcattaaag gtaaacttcg cgaaaacgca aaaactttgg
    18661 ttgaggccgt aaacagatca ggagctacag taatatcgct cgatatcccc tccggtttac
    18721 cgacagaagg ttatatccca agcaattgtg ttgcaataaa agcagattat acagtgacct
    18781 ttggttttcc gaaagtatca atgtttagcc gacgtggtag agagtattgt ggaacagtta
    18841 taaccgcgga cattggcttg ccttcacatg aaatttttaa taataaacca gcttgctata
    18901 tttatgagaa aaaaaatttt agtgatagct ctctgttgta tcaaaatgat gccctgtata
    18961 agacaagagc cggttgcgtc accgtttttg gcggatttcg aggttacgaa ggcgcggcga
    19021 ttttatcagt aaaagccttg agtgaatgcg gtaacggaat gacttatctc gctatagata
    19081 gtgattctga tttgtctggt cgtgaaattc ctcccgaagt catgttgcac cggcttgatg
    19141 cagattataa aatactttgt gattaccttt tagaaaggtc aaaaactgtc gtttgtggtc
    19201 cgggattagg tttgcaagtt aaagccaagt ctcttttgtc acatattctt ttaagggcag
    19261 ccgaaggtaa aattgaaaat ttaattttgg atggtgatgc tcttactatt gttgctgata
    19321 ctgatattaa tttggtaaga aaagcgggca aggcttgccg tctcttcctg actccacaca
    19381 gcatggaagc agcaagactt tctaatgtag atgtagacga gattgacggt gacagtgttg
    19441 ggagtgcaat tgaaatcagt aaaaaatatg atgcgtatgt agttctaaag ggacctgcta
    19501 cggtgacagc atataatgat gaagtaatta tcaatacctc tggtaatcca cttttggcta
    19561 ctggaggatc aggagacgtg cttacaggaa ttattgcttc atttattcgt gatgacgaat
    19621 caattattga ttctgtctct tctgctgttt ttcttcatgg ttattgtgcg gattattatt
    19681 acggcaaata tagaatcaca tcttacaaag cttcggatac tatcaatatg atccgacctg
    19741 ctttaaaatc acttcttgaa agcatagatg accattgatg atgtcattgg ccggcctttt
    19801 tttttgaaat accctaaaaa atgatatttg gcaatagtta gccaacgttt gtagtccttg
    19861 aagtttttaa attgcccacg cgattttcta agtttcggta ttaagccgaa gtcaactgga
    19921 cggtatatct tgatatttgt aaatccgctt ttggtcagaa gggaagacag attgcatgct
    19981 gtaaaatagg aaagatgtcc aggtagaaag tagtggtaat tttgagcttg gctgacagct
    20041 tggaaagcac ccatatcggc agtctgtata acacatagcc cacctggctt gagaatggat
    20101 gcggtttttt taataaaatt cacaggatcc tttatatgct caataagctc aatcatggtg
    20161 ataacatcaa atgaattttc tttaaatggg gcgtcgttca gcgttccttt gtgtactttc
    20221 atattgattg attcgcaata cttagatgcg aagtctgaaa tttcaactcc gtaaacgtca
    20281 taatattttt gtgccgacga tagcagacct ccaaatgagc agcctatatc aagaaaggta
    20341 ccggattttt tatatttatg tatttttttt atacgggctt tccatacata tgaattgaat
    20401 ttatgattat cacgttcgtc gatataagaa taatctgctt ttcctgaata gtagtcatct
    20461 gagtaaaggg aagcaataaa tgtatctgaa aaggctggat tcataaaaat aaataagcag
    20521 ctttggcatt cgtaaacatt aaagctgtgt gattggatga tattgtactt tagagaaatt
    20581 gatgtagagt cgcaaagtgg gcattttttt agaagcggat caaatttcat tttaacctaa
    20641 aaaaaatcaa ataatctgtt tataataata catataattt ataatatggc tttataaaaa
    20701 tagtgcgaaa tgatttttaa atcgtttaga cgggttcgtt gcatataacc gaattcaaaa
    20761 aagctaaaat ttttgaaaag aattttcaaa taattctcgt cagcaaaggt tactgggcaa
    20821 ctttcaagat cgttaacgga tataagccag cagttatcag agagtttttt ttcacgcaca
    20881 agataattgt catcagcttt tctgaatgtg ccaaataatt gtccacccgg tttgagaatt
    20941 ctttctattt cggaaatctg catagtgact ccttcctgtg tatcgtaatg aagagagccc
    21001 caacatattg ctaggtcaaa actgttgtct ttaaatggag atttggaagt atcacagcga
    21061 atagctggta cggcaatatt agagtttatc aatgctgctt tttcagtaat atctgtggca
    21121 aatatgtttt taaaaccaaa atctttcaag agtttaacgt gacgtccgct tccagcccca
    21181 atgtcgattg caagtttatg atcaatatca tttgaatttt tataggatgt aattagtcgt
    21241 acaagattct catcgggata ccctagcagg gatcgttctc tgctatagtg tgtgttccat
    21301 gaagaatcag acagaataat catataacca gttgcctatt tatattgaat atttaatttg
    21361 ttgattgctt tttcacatct ttctgtggca atctcttttg tctttgcaca tgctgcaaca
    21421 acgccaattc tatcatgatt tgtcttgggt gagttgacgg gatcattgct gtttttaaat
    21481 attttaaaga gtgatatctt tgcaaatgca gtatcaagtg gtgtataagt atcgaaacgg
    21541 ccttttttac ctgttatata acgtactatc gatgagtttt ggttcttttt gtaagatggt
    21601 ggagtgaagt tgtcgtcagt catagcagct attgcggcct taaaaaaatt atgtccagtt
    21661 gtatgagaaa ttataacttc aggaatatat tctcccccaa attcggcaac ggcttcaaga
    21721 acataaatat tattatctct tatcttgcat tctaacacaa gcggtgaaca gttgatgtca
    21781 aaagctgttg cagttttttg agctatttct gtaatttcct gataaagact ggaatgttta
    21841 gaaggcgagg agtgcattat gtctacaaag tacggtggag gtgtagttgt tttatcagtt
    21901 atttcataaa gataaaatgt accttttttt actattccga atacaattat ttcatcgccg
    21961 tcgatgtact cttcgattat gagctgtttt ttatctttta tgtaagggtt agtaaattta
    22021 atcagatcat ctaaagaatt aacaagtttt actccagact tggcatgccc aacaactggc
    22081 ttaacgattg cgggaaaatt gtatcctttt tttcgacgag gattgtaaat tgaataatca
    22141 ggcgttagga tattattatc aatgaaaaga gtcttcatct tttgcttgtc aataaagttc
    22201 tcgacgtttt ctggcttgat aaagggaatc gatagtttat cgcacaactc ggcagtagag
    22261 aaaactgcct cggcaaaaga acgagtgagt atggcactga ttttcccatc tagaagtagt
    22321 tcgcgtaatt tttgataaat ttcgtttgtg ttttttattg attcctgaat acgaatatca
    22381 gaaaaaataa aaccaggagc cgcagtatct tggtcgaccg agatcaccct gtagcccatt
    22441 tcccttgcgg ctttaatcaa aggtatttga ttggcacctg ccgcaataga aacaaataat
    22501 ttttgtggtt ttctttttct tttgaaaaaa aacatttaat tgtcagccgt tttttcgatt
    22561 acatacttaa ttgctccaag cgagggcccc tggtcttcgt ggagtacagt atgaaaatgt
    22621 atatcttcaa ttattccttt aaatccgata ttatcaagct ctttttgcat aagtggaaaa
    22681 agaagatctg aagcgtgaga tagtcctcca gcaaaaatta cagcttcggg attaaaaata
    22741 ttaagaagcg aattgactcc aaatgccaga taacgggcgc actgggacat catatgtttt
    22801 gcaaaaatat cccctttctt agcaaaacta tataataatt ctgctgttat tgtttcgcca
    22861 ttaagcctgg aatcggggta gtccgatatc tgttctgttg caagttttgc tattgatgag
    22921 gccgatgcgt atctttcgaa gcatccacgg ttgccacatg agcaagggat gccgtttaag
    22981 tctatactaa tatgaccaaa ctcggcagca aaaccgtctc gtccttcaag aagtcggccg
    23041 tccacaatgg cgccaccgcc aatacctgtt cccagcgtta ccatgatcca gttcgagtag
    23101 tttttaccgt ttccttgcca ccactcccca agcagagccg cggtagcatc attaagaata
    23161 taggtttttt tcccaatttt ttcctcaacc agagaggcaa aattgcaatc attgatcgat
    23221 ggaatgttcg ggcttatcag tattatccct ttttccgagt ctactgaccc tggaattcct
    23281 atgccaacag gaccaagttc ttcaagagag gaatttgtct gatctaaaag accatctaca
    23341 acaagtttaa tattgttaag gatctcttca cggttagatc cggtcggatg ttttaaagaa
    23401 gctgctactt ttccttcttt gtcggttata acggctttga tgttggtgcc gccgatatca
    23461 aatcctatat gcatcgtata ctccgtttat ttttgtctga atattgcatt gaaaataagc
    23521 taattattgc aaaatagcta aattccagca gttttgtagt ccctatagct tattattaaa
    23581 gatcagcata ctttatgaaa aaataaaagt atacagttta aaaatagtta tttgagatat
    23641 ctaaatatat tatgactgac atggaaaagc tgataatata agattctccc ttgataggca
    23701 ctaaaatggt gttaaagttg atttatagtg tgtaaacagt ttaaaattgt caaacaactt
    23761 gttaatgatt cttgtgaaaa cacaaaaaat attgtaataa tacaaaatca agagggctga
    23821 taatattaaa tggctttatg aataatgatt aatttgaatg acaatgtagg gattgaagat
    23881 taatttatgg aaatcgatca aattaggaga atactatgaa agttatagat ttgcatttac
    23941 atagcagctg ttcagacgga atatactccg taaaggaact tgctaaaaaa gcatatcaag
    24001 aaaaaatatc tgttttatca ttaacggatc atgatactgt tattggaata agcgagatga
    24061 aatattacgc caacaaatat agtttatcct ttatttcggg tatagaattt agttgtgatt
    24121 ttgaagatgg ggattatcat cttcttggat atggtattga tcatgaaaat tcagaactga
    24181 ttaaagagat caattattac tcacaggaaa ggcgcaatcg catagataaa ataatatcaa
    24241 aacttaatga taacggaata aaaatcagca aggatgatgt gataaaatgc ggggctgctg
    24301 aaagcccggg aaaaccacat atagctaggg cactcatcat gaaggggtat gctacaaata
    24361 taaatgacgc gtttgatcat tatcttggta aaaatggcat gggtaatgtt ccaaagtcca
    24421 agataagccg tgaaaaagca tactatttga ttaataaaag tggcggtaca gcagttttgg
    24481 cacacccggc atcgttaaac cgaaatgtag agcagttaaa acagataatt gaggatgata
    24541 aaaaaagagg tttatacggg atagagctgt tttctcaaat gcatagcgat ttgcaggtcg
    24601 aacagtatag aaaaatggcg gatgattgtg ggcttgtagt aagtggtgga agtgatttcc
    24661 atggagataa gggagaaacg ctgggatgct atggaaatgg gagaaaaatt ccctttgagt
    24721 ctgtaaaacc tttactggat agatttgaaa tattcgtaaa gaagaattga aactgttgtt
    24781 gaagcattga gagactccac cttgtcggtc atcggaatcg atacagcgtg agagagcagt
    24841 ttgtcaggaa gccccggacc ctctatgccg ggaataacag taaaagtttc tggaaaacta
    24901 aaatcagata ccggtttacc agagctatca agagagatta ttttgacacc gtttcttaaa
    24961 aaataatctg cggtctctat aagagaggga gcatcaaaaa tatccaggtt aaaaacggct
    25021 ccacccgaag ttctaatgga tttataattg taaggatttg ccgcctcagc tgtgacaaca
    25081 atttgagtaa tgccgaatgc tgcggccgag cgcataacgc ttcccgtgtt agccggatcc
    25141 tgaaaaggaa taatcaagga ggggcgcgtc agatcgatag tttccagata tctgtttttg
    25201 gggcattcaa caaaaagaag aagttttttg ctacctgata tgtctaagtc gttataaagc
    25261 gatttcttta gtaataagag ttttgagtct ttgtcaagtt gatctataat ttggtttaca
    25321 ccgctgtttt tttcgctgaa cccatcatag attacggcac agacaatact gtttttaaaa
    25381 taagcaaaat tatcggttat tattttttct ccgcaaacaa gagcaattcc actttttttg
    25441 ggtccagata ttacggcttt ttttaact
//
LOCUS       DAOTGZ010000013        24215 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_3064
            NODE_2434_length_24215_cov_4.661507, whole genome shotgun sequence.
ACCESSION   DAOTGZ010000013 DAOTGZ010000000
VERSION     DAOTGZ010000013.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868088
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 24215)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 24215)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:22:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,785
            CDSs (total)                      :: 2,751
            Genes (coding)                    :: 2,732
            CDSs (with protein)               :: 2,732
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 12 of 19
            Pseudo Genes (internal stop)      :: 5 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24215
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2434_length_24215_cov_4.661507"
                     /isolate="AMR_MDS_3064"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..225
                     /locus_tag="P5123_01955"
     CDS             <1..225
                     /locus_tag="P5123_01955"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRX15059.1"
                     /translation="DTGLYNRNALIERYHSENNVSVRKTILFVIYFMEKDNAPEIFKS
                     LNFEDEELKQYSECLVNDHNSKIYDLSIRF"
     gene            228..818
                     /gene="nadD"
                     /locus_tag="P5123_01960"
     CDS             228..818
                     /gene="nadD"
                     /locus_tag="P5123_01960"
                     /EC_number="2.7.7.18"
                     /inference="COORDINATES: protein motif:HMM:NF000840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate-nucleotide adenylyltransferase"
                     /protein_id="HRX15060.1"
                     /translation="MLQNRIAIFGGTFNPVHNGHLQNASHIADEFQLDRILFVPAKIP
                     VHKRINNDPGPEERSYMLSLAIRADSRFELSTLEIERNSESYSIYTVRSVIKNYPDSK
                     IFFIVGSDSFNTITGWKNYGELISLVHFIVMKRPGDSIREEVLDIIPSVSIAKNVEID
                     VSSTKIREFIKEKKDCAKLLPDKVLSYINEKEFYLL"
     gene            815..1933
                     /locus_tag="P5123_01965"
     CDS             815..1933
                     /locus_tag="P5123_01965"
                     /inference="COORDINATES: protein motif:HMM:NF024791.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0070726 - cell wall assembly [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LCP family protein"
                     /protein_id="HRX15061.1"
                     /translation="MKKIIIALFIALSLALFITNAVVKMNLNRRNIISQLQSESSMIN
                     VLVAGANNFNDNRFSFFMLVSINPDKNSVGLTFIPPTYSVDANDDGSGVKLNSVDFND
                     FSIVSKAIERDIGLKVPFYVSLYSVDAQRITDLVEGISLFIFESTKLLKGLSSGVNYL
                     DGKKSLEYINNVENNSIYYKYDRVMDIVLSLSKNRELYRKFINRPFIERASETLRTNL
                     TIPELESLLQLLVDNTAVHWTLLPGQLEPDGVYSMDEIARKMYRDTFLRKLVVDSETE
                     KNIKVTILNGTTVSGLAMRTRKTLMRDGINVVQFGTYHDQNLQKTLIIDQKGDSERLD
                     LISSKLEIENRHHVIDSTQLHDILVIIGNDLGVLNDEK"
     gene            1920..2288
                     /gene="rsfS"
                     /locus_tag="P5123_01970"
     CDS             1920..2288
                     /gene="rsfS"
                     /locus_tag="P5123_01970"
                     /inference="COORDINATES: protein motif:HMM:TIGR00090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0017148 - negative regulation of
                     translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome silencing factor"
                     /protein_id="HRX15062.1"
                     /translation="MMKNDNKETLIEIKDILEEKKGVDITFMDLSGIHSYLHYFVIVT
                     ANSRIHSRTLSAEVSRKLVEAGFNTRVTPNEPSGWTIVDAFDIVVHIFLEEERDYYNL
                     EYLWADAILPEVASIGGQTV"
     gene            2388..3011
                     /locus_tag="P5123_01975"
     CDS             2388..3011
                     /locus_tag="P5123_01975"
                     /inference="COORDINATES: protein motif:HMM:NF012722.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase dimerization/phospho-acceptor
                     domain-containing protein"
                     /protein_id="HRX15063.1"
                     /translation="MFDKNTNKIIESLTRNLSHEVKNPLTTIKGYAQLIELKSNDEFA
                     LKAVSIMNEQIEAIENQFNEIYSVFQKKHGTDTEFDLVSLVSASVEGVSANLDIEFAS
                     EVFENRITCDQEMLVRMLDIFFTGINFEYFNDISIKVCCGNDNLKIIYNNVSIPEEEM
                     EMLFLPYSSKTLFKQGTAFYELYYICMTENFDITIEKFPSLSFIIKF"
     gene            3011..4432
                     /locus_tag="P5123_01980"
     CDS             3011..4432
                     /locus_tag="P5123_01980"
                     /inference="COORDINATES: protein motif:HMM:NF025885.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54 dependent transcriptional regulator"
                     /protein_id="HRX15064.1"
                     /translation="MNDFQKIVVADDEPSICSLLVDLLSDRYDVITVNRGDHVLDKLK
                     TEGARIVILDINLPGLDGIEVLKQIKEYSSRVTVVMLTASNSVSMATTAMRLGAYDYI
                     VKPFDNDKLLIILKNITDKLQLENEVEDLRGEISKNLKFDTLVGRSREIKSVYATIEK
                     VLNTNSTILISGASGTGKGVLARAIHYNSERSDLPFKTLDCSTIPADLIGSELFGHEK
                     GSFTGAISRKIGKFEAVGSGTLFLDEIGNLSFDMQAKLLMVIQEREFERIGSNTVIPV
                     TARIIAATNRNLPDMVKEGLFREDLYYRLNVVPIFLPPLENRRDDIPEFIEYFLKKYN
                     NEYGRSVSIDMEARVYMSDHSWPGNVRQLENTIRRFVLLADHDKLSKNDVERILVANS
                     DTTDLVQVTSDQQPSYSLQIVNRDGQQRSFEDMEREIILKSLEFNQYNISKTAHHLKL
                     ARKTLHNKIHNYGIVIEKRGMIN"
     gene            4723..6066
                     /locus_tag="P5123_01985"
     CDS             4723..6066
                     /locus_tag="P5123_01985"
                     /inference="COORDINATES: protein motif:HMM:NF021028.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="InlB B-repeat-containing protein"
                     /protein_id="HRX15065.1"
                     /translation="MRIIFKLLVFTLFLMSAVFFSCEESGEGTDNKDKTKPQYFKVIF
                     ESNNGLEVKEQNVEKGKTVKEPDTPIKEGYFFSGWCSDIELTEMWDFNTKVDSDLTLY
                     AKWTDVLPEKFAVTFVSNGGSAVSQQMVIFGATAISPIDPLKSGYVFDGWYSDNSLTT
                     VFDFSETITSDVTLYAKWSPVVINHIVTFESNDGSSVLPQTVSSGGNASRPADPVKGG
                     FTFEGWYSDRLLTTAFDFSDEITSDITLYAKWSPETNSGHTITLTYDGPNVSPRVVYV
                     SWIEDESENVLQNIFICDRILPNLTGKDGLTGVALPFWKQNKYDVNMVDGMTGASTQV
                     SKTVTRTLDIGTTRRFRVYFEIDRSRNGNTYFNDRPSFIYRSSIIDLDNIATEYTLSL
                     YGYMGNGTQSGTFGQHVNVGQNVPGYGPYVFMNSLNYLEPLNDMVTQIKVIVEEN"
     gene            6396..7934
                     /locus_tag="P5123_01990"
     CDS             6396..7934
                     /locus_tag="P5123_01990"
                     /inference="COORDINATES: protein motif:HMM:NF012245.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein"
                     /protein_id="HRX15066.1"
                     /translation="MLKRLILKKFENSHIMEQRRAAALLYFYIIVIGLLFCAGLFTAV
                     LNPSSAVRVISGVSTIAGFIILGISRLKAGNFRQAVNFFLIPTVIQVIMIRFYIGFNF
                     PASGFTSYIYYFLFVIAFAATFSKKISVPLISVLFFVSNIIFYLVVSKGLKDVLLQIS
                     TDGIIHSSAAMIVIALVCYIQINLSQAFMENLKDESNLNKTQLEKIKYLFSSIKNSTQ
                     RLKDASEKFSVTSQSISSGAREQSAVLEESSAAMEEMTATISMISDDITKQAKTIDDV
                     DKSVNNLSELIAQVASHAEHIMKESNRSIREGDNAASNSSLAMDGIKRIQQSSEKIRD
                     IILLISKLADQTNLLALNAAIESARAGDAGYGFAVVADEISKLAENSTSSAREISDLI
                     NETSSVIAQNYDFFSGFEKQIHDMRDTLRNTANLSKQMNEAAHNQLGFSNNVKSSMNM
                     INSVVSKIADATKEQTQTSLTLSRSLESASEITQSYAINAEDLNTITQELKNITKVLN
                     EEMK"
     gene            complement(7959..8639)
                     /locus_tag="P5123_01995"
     CDS             complement(7959..8639)
                     /locus_tag="P5123_01995"
                     /inference="COORDINATES: protein motif:HMM:NF014244.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-2 protease family protein"
                     /protein_id="HRX15067.1"
                     /translation="MDYTNDNIIRVIIAASWVIPLLFSVIPHEVAHGYIALRLGDNTA
                     KEMGRISLNPLRHIDFYGTIVIPAFLILSNTGFVFGYARPVPVNFANFKNYRKGMILV
                     SLAGPLTNFILAAIFLAIIIMLKSMSIPKNIISVFLYTSFVNTALLNTVLGIFNLIPL
                     PPLDGGKIVIGLLPQKAAAKYAKLDSKAGFIILIILLILPRIFGDEYNLIGRFISFFT
                     DSLFNLVF"
     gene            complement(8717..10042)
                     /locus_tag="P5123_02000"
     CDS             complement(8717..10042)
                     /locus_tag="P5123_02000"
                     /inference="COORDINATES: protein motif:HMM:NF024206.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ankyrin repeat domain-containing protein"
                     /protein_id="HRX15068.1"
                     /translation="MKLLLQNKRKTILFVIAFLFFITITADDSAKTSTIRWESSPGAI
                     GYMVTLKDSKGKIIINKRVDTNAANIPVTGGSYFIRIQPINKFNKIDSEDSSWQKISV
                     KTIGRPFINSITPDYLTPDSRENRITANGKNFTEGMSVTLVNDKTRHNVSSMAILSDK
                     RFTFSFNPSLSGSGLFKIEITTADGTKFITDSSIAIGLEAAALRHSSEKSPSSVASGD
                     NLIYYIIKGNNSKVEELLKAGVNPDTTDKNGFYAVHYAAFFGRALILAKLKNYGANLN
                     VSDKGGAYPIHHAAYKDRLDTVNYIVINGLNDVNSRARNGYAPLDRAAIKGNSRIIDI
                     LIKSGAKPDYTFNSGENALHMAVYSGDMDSVKYLLATGKIDVNYQEKRGYTVLHYAAW
                     FGHPEICDYLIRHGADKNVKSKKGETALMLAENTRKKSNRDKIIELLKE"
     gene            complement(10020..12107)
                     /locus_tag="P5123_02005"
     CDS             complement(10020..12107)
                     /locus_tag="P5123_02005"
                     /inference="COORDINATES: protein motif:HMM:NF016648.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FecR domain-containing protein"
                     /protein_id="HRX15069.1"
                     /translation="MRTIKRRKKEIALVLFCLTAAVISSGLLYRSFSGTNESITKPIG
                     SVAFKVKDVQRKLFKSDLWEIVYNNSEVYNKNKVKTSENSFVSINLHSGSEIELDENT
                     LVYLEFDGEITTIELLEGSIHTKNVDSASKLRIKSDKTVIDSSNSSASFYKDKDGSIK
                     LTVHDGEVKISSADGTKNVKAGDSALVDEEEITVIKNMIRIDSPTSNRYYISNINDAN
                     ISFSWTDPANDGPYVLLISNNSSFDKYNTYNSKSKSIDLKLETGKYYCMVKNDSGDKQ
                     SSVIKFSIIYDSKPYLILPENNGTIDSYSTNPVISFSWGASTMPSKYTVEIYSDSELK
                     NIVIKKDTETKSIDADTLTYGTYYWLVKAHYSISPDDTIVSDIGSFQINKSSTISQPV
                     LLQPLNNETFSSDFFKKNRIMFSWNRNADIAQYEIQISTDMDFNSIYYESNTINSSIK
                     AEKHMPPGTYYWRLKYFDEAKKKEVYTDIRSFIVISETNIEPLSPGNRAVISSDRTIR
                     IHWKDPSMWNNYKVELSTDHEFRDIRESIVTRNNYADVHVAEGTYFWRVSRLDDKGRP
                     ISTYSGGTFAVSSSVTEETSRITIIEPKNGSIIDMTNKSVIRISWRDEDKPDKYKVEL
                     IQNGIVKFLTETTKTSLNFAELHKLDRGNFSIRITSVVSKKSGAYTETDFEIVLKPLK
                     KPELITDEIIITE"
     gene            complement(12183..13784)
                     /locus_tag="P5123_02010"
     CDS             complement(12183..13784)
                     /locus_tag="P5123_02010"
                     /EC_number="4.6.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012437.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009975 - cyclase activity [Evidence IEA];
                     GO_function: GO:0016849 - phosphorus-oxygen lyase activity
                     [Evidence IEA];
                     GO_function: GO:0032555 - purine ribonucleotide binding
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate/guanylate cyclase domain-containing
                     protein"
                     /protein_id="HRX15070.1"
                     /translation="MRHISQFKLLFIISLVLITALLSLLFLSSSFYKSELDNRVTEEL
                     LLRSSLIASSFQTSFESFESVAKYIDSDFSSSNELFNISSSIISITITDYDNKTPKTS
                     INPIYESSGMVSLKKIADAVNINFKRILYVTKNRTLITDFSEQLGTPALSLAISTDLQ
                     ENPDRIVIITFDPRPSFGQLPSNGLINSFLVNSQGAVIAAETTENLEYTKKTGLLDNI
                     DKIFTAVADNRVSLYQDNNSKEYYISHKRIGQHECAAVSIALRDNLLDSASMILYRNI
                     LIMLIIIALSVLCSFIFLKGASNPINKGSSKNRKSGKTSSFSTKHDKALLAYFNICSL
                     SSLATLYKTETALALINNCEKRFNNAIRSTNGVKSASLGDVFTVFFKSTKSVKHGCHN
                     AALCALMIRKEIFKFNTKYKLKKPLKMRCGIDNGIFVTGTGDLSYNKTLGIIGDTVKK
                     VSQIEHLNDYYETDILVSEYIYSLLKDKFYFEAVKPIILKGSSQKLLLWALLGLKKDS
                     NAPKNISELQKRIARVSNKSNIENK"
     gene            14036..15088
                     /gene="selD"
                     /locus_tag="P5123_02015"
     CDS             14036..15088
                     /gene="selD"
                     /locus_tag="P5123_02015"
                     /EC_number="2.7.9.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010528032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004756 - selenide, water dikinase
                     activity [Evidence IEA];
                     GO_process: GO:0001887 - selenium compound metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="selenide, water dikinase SelD"
                     /protein_id="HRX15071.1"
                     /translation="MDYPEFDLLTTVETGGCSAKLPAGALKELLAGIPQLKSEKLIIG
                     NDCSDDALVFKINDSTALIQTTDFFPPLCSDPYTFGYIAAVNALSDVFAMGGEALTAL
                     NIVMFPTASIDKSVLKSILTGGADAAAEAGAVLAGGHTIDGPVPVYGLSVTGSVHPDR
                     IMRNSGAKPGDLLILTKGIGTGALTAALRTGEIVAEDYSDALLSMKQLNMVASRILQE
                     FDVCAATDITGFSLAGHALEMALASKVHLKMYSEEIPLLDNAYRVTETGCIPCASFRN
                     LKFVEDRVQFSDKVDYNRKMLSVDAQTSGGLLVSINADKATDLNNALLAAGYDYSTII
                     GEVFESGYGNTPIEVV"
     gene            15089..16114
                     /gene="mnmH"
                     /locus_tag="P5123_02020"
     CDS             15089..16114
                     /gene="mnmH"
                     /locus_tag="P5123_02020"
                     /EC_number="2.5.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF008750.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 2-selenouridine(34) synthase MnmH"
                     /protein_id="HRX15072.1"
                     /translation="MAIYLEPEPFLEKSAFLKIIDVRSPGEFEAGHIPDAVNIALFDN
                     EERAVVGTIYKQQGRELAVLKALEIVGPKLVGYVTEAQKYVVNGEILVHCWRGGMRSE
                     SFAWFCETIGLKVYLLKGGYKSYRHYIRQSFLLCDRLVVLGGFTGSGKTEILNELGKS
                     GEQVIDLEGFANHKGSAFGAIGQKEQPTTEQFENNLAQEWLKFDMRKPIFIEDESLKI
                     GTVVINENLYSRMRSTKLYVVDIPRSERIKRIERDYIIVPAEKLVSIVKRIERRLGGD
                     RCRECCELFVSGDYKGAIDILLVYYDKGYSHGMSKRNQDMVHSVKLESGDPVKAAKAL
                     KDAVYNG"
     gene            complement(16127..17173)
                     /locus_tag="P5123_02025"
     CDS             complement(16127..17173)
                     /locus_tag="P5123_02025"
                     /inference="COORDINATES: protein motif:HMM:NF012678.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030554 - adenyl nucleotide binding
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron-containing alcohol dehydrogenase family
                     protein"
                     /protein_id="HRX15073.1"
                     /translation="MEIVVPELVRIKPQALQRLGKYLRTSNCSEIALFWGDGLKQLFN
                     STVEVSLQSSGITVLHEKNVNTTSIEEIFENSLDIPSATSAIVAIGGGQVIDYCKYIS
                     FITKKKLFTVPTIISNDSFCSPVASLYVRNKKRSISTNLPYSVIIDTDILQNAPQKYI
                     FSGMGDLLCKTTAIFDWKLSYKKTGEYVNDFSVVIVRTALDAFINFPDKDLSNPDYVR
                     TIGSSLMMTGIAMEIAGISRPASGSEHLISHAYDKISKSPSLHGIQTGIAAYAVSFVQ
                     KETHNEIRKSMESCGFDKFAAENPLNREELITAVRHAPSIKENFYTILSQPGALEEII
                     NFINSDELMRSMAV"
     gene            complement(17199..17993)
                     /locus_tag="P5123_02030"
     CDS             complement(17199..17993)
                     /locus_tag="P5123_02030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020063446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IIA family hydrolase"
                     /protein_id="HRX15074.1"
                     /translation="MSLAIISDMDGVIYRGGNLVDGAKEFVSALKEKNIPFLFLTNNS
                     EQTQIDLVLKLKNMGIEGLTEDNFITSAMATATFLKSQKKSGTAYIIGGAALSNELYK
                     AGYSLTETNPDYVIVGKTKSLNFDMLRRAINLINAGAKFIATNPDIIDPVENGIEPAC
                     GTIIAAIQAATGKAPYIVGKPNALMMMVAKQMLGVHSAETVMIGDRMDTDIISGLEAG
                     MRTCLVLSGVSTISTLDTFPYKPTYIFNNVGEIDLNLFEKETPDNL"
     gene            18372..20615
                     /locus_tag="P5123_02035"
     CDS             18372..20615
                     /locus_tag="P5123_02035"
                     /inference="COORDINATES: protein motif:HMM:NF012551.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endo-1,4-beta-xylanase"
                     /protein_id="HRX15075.1"
                     /translation="METRFYFRTFHMIAIIVLITAFFGGCTKKSDLRKAAEKQGILIG
                     TAVDTRALEDEKYYKTLVQNFNVVTAENVMKWETIHPRRDVYDFTEGDKLLAFAKKHG
                     MKMRGHTLVWHNQNPNWLLSQPWKADELYKVLEEHIKTVVTHYKGEVYAWDVVNEALD
                     VDGYRETLWYLYLGPEYIEKAFRWAHEADPDALLYYNDYSNGEINDKSNYMYNMLKDL
                     QADGVPVHGVGLQMHLSLEFGFDFESLYMNVNRLHELGLHVDYTEIDVRIKTPASESD
                     YREQAMRYKKMMEIMLAFDNSYAYTVWGVSDAHSWVPSAFRGTGDALLFDKEIEPKQA
                     YHMLVETLEKGPVELNYSKNYEDKFRSRKAIPPFRAHAVASAPKIDGNPNDKAWEKGV
                     TYPFNYNQLNLYDQRIPDSNDLWGEWTVVYKGNTIYGIIRRNDEKTRTDHKLDYENDN
                     IEVFFHLGDVFAQLRSRVGHDWESHAYDGERKIAWNKEGTVCEYSVTLPIDDLTGMIS
                     GWNISLSDNDGKENRDHQLYPINGQNDGWQGKNLGTMQYVGDSPRVPEDPRVVMPFIA
                     APASSEPVLDGSFSDDEWIDAVKYQFAYNQINPKKQSIGLKNRAISGSWGIVRKGNYL
                     YGFVLRNDAKTVTSNADPALNDNVAIYLEIEDGNAVVMRTVVGKDWEESGLNGEHQAK
                     WSDDGSVLEFRIELPSNVASRKMIGFNIALSDNDGGDKLDYKLYPITGDDKSQEAKGL
                     AELELQL"
     gene            complement(20710..21438)
                     /locus_tag="P5123_02040"
     CDS             complement(20710..21438)
                     /locus_tag="P5123_02040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012548158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HRX15076.1"
                     /translation="MNKTIFLLLITTICTSCSKKDIDPLTGEPYPTANKICALTFDDG
                     PSNDPELTTRVLDTLEKNSVKATFFLVGQNFNDSTREIVMKISKSGHEIGNHSWTYDG
                     MQDMSAQQVKTYVKKTSDIIEEYAGVKPKFFRAPNLSASKTMYESIDLPFASGVLGFD
                     WPGGGGDTPEKIIDHVMSGMRDGAIILLHDVQPKPHPTPEAIETLIPKLKREGYEFVT
                     LSELFERKGIKPDSSSGFMYVYAE"
     gene            complement(21556..22674)
                     /locus_tag="P5123_02045"
     CDS             complement(21556..22674)
                     /locus_tag="P5123_02045"
                     /inference="COORDINATES: protein motif:HMM:NF024242.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HRX15077.1"
                     /translation="MNKNKILILLLFIFLSISISIAYLIFTASRNIFPETDTRIYPIT
                     DHNDSGNSEIISINTNNGQIDLQYILRDGFAFPYAGVGIEILSSVPLYSSGFDFFEIT
                     AKTSKKTSLKINIITKVNLSDKKNTSYPHKISYYYYTSIIDITPEFSTIRVPLSDFLT
                     PEWFLVKHKIEKKSIDSIGKNPLWSIEILNSSIDELNTPLGISITYFGFKKDIFYYSI
                     LSMLIISVLALLGLFLSHILKSHKAVKYAQDIIRIHSNLNLGNEKEREAGKLFEYLAA
                     NYNDPYISVQQISEALNLSPYKIPKIINKYTNLSFKQYINLIRVDEAKNLLINSDSKI
                     IDIAFAIGYNSIAHFNKLFKMQESLSPKEYRQKYQTGN"
     gene            complement(22701..23573)
                     /locus_tag="P5123_02050"
     CDS             complement(22701..23573)
                     /locus_tag="P5123_02050"
                     /inference="COORDINATES: protein motif:HMM:NF018963.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1460 domain-containing protein"
                     /protein_id="HRX15078.1"
                     /translation="MKDIYSSFPVFSALILLMLIMPTSVNCSADNIELQSRERSGQRT
                     LFNRIMQSSTGRSTSLTFLMDYFTGTPYVPHTLENNETEKLIVRLDALDCVTYVETVT
                     ALALMFATTDHSYESFCNHLTNIRYRNGIIDGYSSRLHYFSDWLHENERRGFISNITK
                     KLGGKPDTRIISAMSSSDKRYPMLKEKKQLAKIRNIEANLSKKTFYYLKTIEVKQKSH
                     LIPDGAIIAIVTSKRSIFISHVGFARFINGKLMFQHASFSHKKVVISKKNLHDYLVSD
                     KSRIGIMVAVITTK"
     gene            complement(23747..23819)
                     /locus_tag="P5123_02055"
     tRNA            complement(23747..23819)
                     /locus_tag="P5123_02055"
                     /product="tRNA-Asn"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(23784..23786),aa:Asn,seq:gtt)
     gene            24008..>24215
                     /locus_tag="P5123_02060"
     CDS             24008..>24215
                     /locus_tag="P5123_02060"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRX15079.1"
                     /translation="MDTLELPDYNREEEEGISDQKLEELRKKTFTPDLTGLISRVTSH
                     NEDQSVTTDSEIESVSTDDRFNLLK"
BASE COUNT         7667 a         3985 c         4762 g         7801 t
ORIGIN      
        1 gatacaggac tttataaccg taatgccctt attgaaagat accattccga aaataatgtt
       61 tcggtgcgca aaaccatact gtttgtgata tatttcatgg aaaaagataa tgctcccgaa
      121 atattcaaga gcctaaattt cgaagacgaa gagctaaaac aatattccga atgtcttgtt
      181 aatgatcata attccaagat ttatgatcta tcaataaggt tttaatcatg ttacaaaaca
      241 gaattgccat tttcggcgga acatttaatc ctgttcataa cggccatctg caaaatgcga
      301 gccatatcgc agatgagttc caacttgaca gaattctctt cgttccggct aaaattcctg
      361 ttcacaaaag aataaataat gaccccggtc cagaggaacg ttcttacatg ttaagccttg
      421 ctattcgggc agacagtcgt ttcgagctgt ctactctcga gattgaaaga aatagtgaat
      481 catattcaat atatacagta agatcggtta ttaaaaacta tcctgattcc aaaatattct
      541 ttatagtagg aagtgacagt tttaatacaa ttaccggatg gaaaaactat ggagagttga
      601 tttcacttgt tcattttatt gtgatgaaaa gaccgggtga cagcattcga gaagaagttc
      661 tagatataat accatctgtt tcaattgcga aaaatgtaga aattgatgtc agctcaacca
      721 aaattaggga atttatcaaa gaaaaaaagg attgcgcgaa gctgctacct gataaagtat
      781 tatcatatat taatgaaaag gagttttacc tgctttgaaa aaaattataa ttgctctctt
      841 tattgctcta tcccttgcac tgtttattac aaatgctgtt gtaaaaatga atcttaaccg
      901 acgtaatatt atttctcagt tacagtcaga gagcagtatg ataaatgtac ttgttgctgg
      961 ggcgaacaat tttaatgata acaggttttc gttttttatg ctagttagca taaaccctga
     1021 taaaaatagc gtaggtttaa cttttattcc gcctacatac agtgtagacg caaacgatga
     1081 cggatccggg gtaaaactta acagtgttga ttttaacgat ttttcaattg tatcaaaagc
     1141 aatagaacga gatattggct taaaggttcc tttttatgtt tcgctatatt cggtagatgc
     1201 gcaacgcatt accgaccttg ttgagggcat aagtcttttt attttcgaat ctacaaaatt
     1261 actaaaaggt ctttcttctg gtgtcaatta ccttgatggt aaaaagagtc ttgaatacat
     1321 aaacaatgtc gaaaataatt cgatatacta taaatacgac cgagtgatgg atattgttct
     1381 ttctctttct aaaaatcggg agctttacag aaagtttata aacagaccat ttatagagcg
     1441 agcctccgaa actttacgca caaaccttac tatacccgag cttgaaagtc ttttgcaact
     1501 tttggtagat aatacagctg tacattggac ccttttacct ggccagcttg aacctgatgg
     1561 ggtctatagt atggacgaga tagcccgaaa aatgtatcgc gatacgtttt tacgtaaact
     1621 tgttgtcgat tctgaaacag agaaaaatat caaggttaca attcttaatg gaacaacggt
     1681 gtcgggattg gcaatgagga caagaaagac cttgatgcgt gatggcataa atgtggtaca
     1741 gtttgggaca tatcatgatc aaaatttgca aaaaacactt attattgatc aaaaagggga
     1801 ttcggaacgt ctggatttaa tcagttcgaa acttgaaatt gaaaaccggc atcatgtaat
     1861 tgacagcacg cagcttcacg atattcttgt aatcattgga aatgatttgg gagtcttgaa
     1921 tgatgaaaaa tgataataaa gaaacactga tcgaaataaa agatattctg gaagaaaaaa
     1981 agggtgttga tataactttc atggatttaa gtggaattca ctcctatttg cattattttg
     2041 taattgtaac agctaattca agaattcata gtcgaactct gtctgctgaa gtttcaagaa
     2101 aattggtaga ggctggattt aatacccgtg ttactccgaa tgagcctagt ggatggacta
     2161 tagttgatgc tttcgatata gttgtgcata ttttcttaga agaggaaaga gactattata
     2221 atctggaata tttgtgggca gatgctattt tgcccgaagt tgcaagtatt ggtggtcaaa
     2281 ccgtataata ttatgcagcg tagacaattt caatggctat cattgttaag atagtctctt
     2341 gatatataca attgaagtga tttgttttaa tccaaagaag gacatttatg tttgataaaa
     2401 atacaaataa gattatcgaa tcattgactc gtaatctgtc acacgaagta aaaaatccgc
     2461 tgactacaat aaagggttat gctcaactta tagagttaaa atcaaatgac gaatttgcct
     2521 taaaagctgt aagcattatg aatgaacaga tagaagcaat tgagaatcag tttaacgaga
     2581 tttactctgt tttccaaaaa aaacatggaa cagataccga gtttgacctg gtatccctgg
     2641 tgtcggcttc tgtagaaggt gtatctgcta atcttgatat agaatttgcc agtgaagtct
     2701 ttgaaaatag aattacatgt gatcaggaaa tgcttgtaag aatgcttgat atctttttta
     2761 ccggaattaa ttttgaatat tttaatgata taagcattaa agtatgttgt ggtaatgata
     2821 atttaaaaat aatctataat aatgtttcga ttcctgaaga agagatggag atgctttttc
     2881 tcccatatag tagtaaaact ctatttaaac agggtactgc tttttatgaa ctttactata
     2941 tttgtatgac agaaaatttt gatataacaa tagaaaagtt tccatcgctc tcctttataa
     3001 ttaaattctg atgaatgatt ttcaaaaaat agttgttgcc gacgatgaac catcaatttg
     3061 ctctctgcta gttgatcttt tgtctgatag atatgatgta atcacagtga acaggggtga
     3121 tcacgtccta gacaagctga aaactgaagg agctcgaata gtaattctgg atattaacct
     3181 gcccggtctt gacggaattg aagttttaaa gcagataaaa gagtactcct cccgcgttac
     3241 tgttgtaatg ctcaccgcgt caaacagcgt ttcaatggct actactgcaa tgcgacttgg
     3301 ggcctatgat tatattgtta aaccttttga caatgataag ctgttaatca tccttaaaaa
     3361 cattacagat aagttgcaac ttgaaaacga ggtcgaagat cttagagggg agataagcaa
     3421 aaatcttaaa tttgatactc ttgtgggtag atcccgtgaa ataaaatcag tttatgctac
     3481 aatcgagaag gttttaaata caaattccac aattctgatc tctggagcaa gtggtacggg
     3541 taaaggggtt ctggctcgtg ctattcacta taatagtgag agaagtgatt tacccttcaa
     3601 gacgcttgat tgtagtacta ttcctgctga ccttataggg tcagaactat ttggccacga
     3661 aaagggaagt tttaccggtg ccatatcccg aaaaattgga aaatttgaag ctgtaggtag
     3721 tggcactctt tttctggacg aaattggaaa cttaagtttt gatatgcagg ccaagttgtt
     3781 gatggtgata caggagagag agttcgaaag aattggaagc aatacggtta ttccggtaac
     3841 agcccgtatt attgcggcaa caaaccgtaa ccttccagac atggttaagg agggactttt
     3901 cagagaggat ctctattatc gccttaatgt agttccaata tttttgccac cactcgaaaa
     3961 ccgaagggat gatatacccg aatttataga atactttctt aaaaaatata ataatgaata
     4021 tggacgttct gtttcaattg atatggaggc tcgtgtttat atgtcagacc attcttggcc
     4081 tggaaatgtg cgtcagcttg aaaatacaat acgcagattt gttctacttg ctgatcatga
     4141 taaactatca aaaaatgatg tcgaaagaat acttgttgca aacagtgaca ctactgattt
     4201 agtacaggtg acatcggatc aacaaccatc ttattcgtta cagatagtaa atcgtgacgg
     4261 acagcagcgt tcgtttgaag atatggaacg tgaaattatt ttgaagtcac ttgaatttaa
     4321 tcaatataat atttccaaaa ctgcgcacca tcttaaatta gcccgcaaga cgcttcacaa
     4381 caaaatacac aactatggta tagtaattga aaaacgaggc atgattaact aaatccgtca
     4441 agttatgctc tgttattaca gatcgatgtg tatgttcatt cctatcattt tgtaaatact
     4501 tagtcacaat ttattgcaaa acattaagaa tatttcttaa acatattgag ttgtatacaa
     4561 taaaaaaccg acaatccata cggattgtat attcaaaaaa aaagagttga ttatattttc
     4621 aggaaacaat ataaggcgag tcaatacagg gttagttaat tatcttgcct aattattccg
     4681 acacccctga ccttgagtct aattaatggg ggccacacaa tcatgagaat tattttcaaa
     4741 ctacttgttt ttacactttt tttaatgtcc gctgtttttt ttagctgtga agaaagtgga
     4801 gagggtactg acaataagga caaaacaaag ccgcaatatt tcaaagttat atttgaatca
     4861 aacaatggtt tagaagttaa agagcagaac gtagaaaagg gcaaaacggt taaggaacct
     4921 gatactccaa ttaaagaagg atacttcttt tctggttggt gttctgatat tgaattaacc
     4981 gaaatgtggg attttaacac aaaagttgat tctgatctta cgttatacgc gaagtggaca
     5041 gacgtattgc ccgaaaaatt tgctgtaact tttgtttcaa acggtggaag tgctgtgtca
     5101 cagcaaatgg taatatttgg tgctacagca attagcccaa ttgatccgtt aaagagtgga
     5161 tacgtttttg atggatggta ctccgataat tcgcttacaa cagtctttga tttttcagaa
     5221 actataactt cagatgttac attatacgcg aagtggagtc ccgtagttat aaatcatatt
     5281 gttacttttg aatcaaacga cggaagttcg gtgttaccgc aaactgtttc tagtgggggt
     5341 aatgcatccc gtccggctga ccctgtaaaa ggtggattta cctttgaggg ctggtattct
     5401 gacagattac ttacaaccgc ttttgatttc tcggatgaga taacctcgga tataacttta
     5461 tacgcgaagt ggagtcctga aacaaattct ggtcatacca taacattaac ttatgatggg
     5521 ccaaatgtta gtcccagagt tgtttacgta agctggatag aggatgagag tgaaaacgta
     5581 cttcaaaata tctttatttg cgatcggata ttacccaatc ttacagggaa agatggttta
     5641 acgggagttg cattgccttt ctggaaacaa aacaagtacg atgttaatat ggtagatggt
     5701 atgaccggag cttctactca ggtaagtaaa accgtaactc gtacgctgga tattggaaca
     5761 acaaggcgtt tcagagtcta tttcgagata gacaggtcgc gtaatggcaa tacatatttc
     5821 aatgatagac catcgtttat atatcgctcg tcaattattg atcttgataa tattgctaca
     5881 gaatacacat tatcgctata cggttatatg ggcaatggca cgcagagtgg aacatttggc
     5941 caacatgtga atgtggggca aaatgttccg ggttatggac cttatgtatt tatgaattcg
     6001 ttaaattatc ttgaaccctt aaatgatatg gttacacaaa taaaggtaat agtagaagag
     6061 aattaaaatc ctgtaagctt tcaataattg attatccgga tacagatttt tctaatttgt
     6121 gtccggatac tgtaatacat acttttttgt attagacaaa ttcccaaaac atcacaaaca
     6181 tgatgcttga taaaaatata gcatggttgt gcttgtatta atttatattt attgacatca
     6241 gctgtatatt gatctataca tttctacgtg gtacagagta attgttttac taccataatc
     6301 agacataatc atataaaaac cgtcattaac ttaatttact ttggaattag aagtgagtat
     6361 tcgaaattat aatgtataaa tattaaggtg tggatatgct taaaagatta atactaaaaa
     6421 aatttgaaaa cagtcatata atggagcaac gtagagccgc ggctctgctt tatttttaca
     6481 ttatagtaat aggtctgctt ttttgtgcgg ggctatttac tgctgtttta aatccctcaa
     6541 gcgcagtaag agtaatctcg ggagtaagca caatcgcagg ttttattatc cttggcataa
     6601 gcaggttaaa agctggaaat tttagacaag ccgtaaattt tttccttatt cctactgtta
     6661 tacaggttat tatgatcagg ttctatatag gttttaactt tccagcatca gggttcacct
     6721 cttatatcta ctattttttg tttgttatag cttttgccgc aacattcagt aaaaaaattt
     6781 ccgtgccgct tatttcagtg ctattttttg tatccaatat aatcttttat ctggttgtaa
     6841 gtaagggact taaagatgtt ctgttacaga tttctactga tggaattatt cacagctctg
     6901 cggcaatgat cgttattgct ctggtatgtt atattcagat caatttatca caggcattta
     6961 tggaaaatct gaaagatgaa tcaaacctta acaaaacaca gcttgaaaag ataaaatatc
     7021 tattctcttc aattaaaaat tcaacgcaaa ggcttaaaga tgcgtccgag aagttttctg
     7081 ttacttcaca atccatctcc agtggtgcac gtgaacagtc ggctgttctc gaagagtcat
     7141 cggcagccat ggaagagatg acagctacaa tatcaatgat cagtgatgat attactaaac
     7201 aggcaaagac aatagacgat gtagataaat ctgttaataa tctgtcagaa ctaatagctc
     7261 aggtagcctc gcatgccgaa catatcatga aggagtcaaa cagatctatc agggaagggg
     7321 ataatgcggc gtctaactct tcattggcaa tggatggtat aaaaagaatt cagcaaagtt
     7381 ctgaaaaaat acgtgatatt atacttctta tttcaaaatt ggcagaccag acaaatttgc
     7441 ttgcccttaa cgctgcgata gaatcggcaa gagcagggga cgcgggttac gggtttgctg
     7501 ttgttgccga tgagatctca aaattagctg aaaacagtac atcgtcggca cgagaaatat
     7561 cagatttgat aaacgaaaca tcatctgtta tagcacaaaa ctacgatttt ttctcaggat
     7621 ttgaaaaaca gattcatgat atgcgggaca cattgagaaa tactgctaat cttagcaaac
     7681 agatgaacga agcagcacat aaccagctgg gttttagtaa taatgttaag agttccatga
     7741 atatgataaa ctctgttgtc tccaaaatag cagacgcaac aaaagagcag acacaaactt
     7801 ctcttacact atcaagatca cttgaaagtg caagtgagat tacacagtca tatgcgatta
     7861 atgcagaaga tttaaataca attacacagg aacttaaaaa tatcacaaag gtacttaatg
     7921 aagagatgaa gtaaaaaaaa tagagagtac aatatgtctt aaaaaacgag gttaaataaa
     7981 gagtctgtaa aaaaactgat aaacctgcca atcaggttat attcgtctcc aaatatacgg
     8041 ggaagtatca agagaataat caaaataata aatccagctt tactatcaag cttggcatat
     8101 ttcgcagcag ctttttgagg aagcaaacca attacaattt tacctccatc cagaggaggc
     8161 aacggtataa gattgaaaat acccagaact gtatttaaaa gggcagtgtt tacaaaactt
     8221 gtatataaaa aaactgaaat gatatttttg gggatagaca tagattttag cataatgata
     8281 attgctaaga atattgctgc caaaataaag ttagttaatg gacctgccaa cgaaacaaga
     8341 atcatgcctt tgcgataatt cttgaaattt gcaaaattta caggaacagg tcgggcatat
     8401 ccgaaaacaa atccggtatt ggaaagaatt aaaaacgcgg gtataacaat agtgccataa
     8461 aaatctatat gtctaagcgg attaagagaa attctgccca tctcttttgc tgtgttgtct
     8521 ccaagacgta aagcaatata gccatgagcc acttcatgag gaatgacaga aaatagtagt
     8581 gggataaccc aagatgctgc aattattact ctgatgatat tatcgtttgt atagtccatc
     8641 acctattgat aaagtatact gttttcaagt caactgtttt gcttgtaata acaagttcac
     8701 gaaaataata agtaaattat tcttttaaca actcaattat tttatctcga ttagacttct
     8761 ttctagtgtt ttcagccagc attaaggctg tttctccctt tttcgatttt acatttttat
     8821 cggctccatg acgaataagg tagtcgcata tctcgggatg cccaaaccag gcggcatagt
     8881 gtaaaacagt ataacctctt ttctcctgat aattgacatc aattttacct gtggcaagta
     8941 aatattttac cgagtccata tcgcctgaat agacagccat atgaagagca ttttctccgc
     9001 tgttaaaagt gtagtctggt ttggctccac tttttattag tatatcaata atgcggctat
     9061 ttccctttat tgccgccctg tcaagcggag catagccgtt tcgtgctctg ctgttaacat
     9121 cattaagacc atttataact atataattga cagtgtccaa tctgtctttg tatgcggcat
     9181 gatgtattgg ataagcgcct cccttgtcac tgacgttaag gtttgcaccg taatttttca
     9241 acttggcaag aatcaaggct ctaccaaaaa aagccgcata gtgaactgca taaaaaccgt
     9301 ttttgtcagt tgtatcagga tttacgcctg cttttaatag ctcttcgact ttggaattat
     9361 taccttttat aatataatag atcaggttat cgcccgatgc gactgaagac ggacttttct
     9421 ctgaagagtg ccgcaaagca gcagcttcaa gaccaatcgc aattgatgaa tcggtaatga
     9481 atttggtgcc atcagcagtt gtaatctcta tcttaaaaag accagaacca gacaacgaag
     9541 ggttaaagga aaatgtaaat cgtttatcag ataatattgc cattgatgaa acattatgtc
     9601 ttgtcttatc gttaacaagt gttacactca tgccttctgt aaaatttttt ccgttagctg
     9661 taattctgtt ttcacgggaa tcaggggtaa ggtagtcggg ggtaatcgag ttgatgaagg
     9721 gccgtcctat tgttttgact gatatttttt gccatgaact gtcttctgaa tcgattttgt
     9781 taaatttgtt gattggttgt attcttataa agtatgatcc accagtaacg ggtatattgg
     9841 ccgcatttgt gtctactctt ttgttgatga taattttgcc tttgctgtct tttaacgtta
     9901 ccatataacc aatagcaccg ggtgaagatt cccatcttat tgtagaggtt tttgcagagt
     9961 catcagcagt aatagtaata aaaaacagga aagctattac aaaaaggatg gtttttcgtt
    10021 tattctgtaa taataatttc atcagtaatt aactccggtt tttttaacgg ttttaagact
    10081 atttcaaaat cagtttctgt gtatgcgcca gattttttac tgacaacaga tgtgattcta
    10141 atggaaaaat ttcctctatc tagtttatgc agctctgcaa aattaagact ggttttggtg
    10201 gtttcagtta aaaactttac aattccgttt tgaataagtt ccaccttgta tttgtctggt
    10261 ttatcttcat ctctccatga tatgcgaatt acagatttat tggtcatatc aataattgat
    10321 ccgtttttgg gttcaattat ggttatacgt gacgtttctt cagtcacact actgcttaca
    10381 gcaaaagttc caccagaata tgtcgaaatt ggtcgaccct tgtcgtcaag gcgtgaaaca
    10441 cgccaaaaat aggttccttc cgctacatgc acatctgcat aattatttct tgtaacgatt
    10501 gattcacgta tatctctgaa ctcgtgatct gtagaaagct caaccttata attattccac
    10561 atggatggat ctttccagtg aatacgtatc gtgcgatcac tggagattac ggcacgattg
    10621 ccaggcgaca aaggctcaat attggtttct gagatcacta taaaagaacg aatatctgta
    10681 tacacctctt ttttcttggc ttcatcgaaa tattttagtc gccagtaata tgttccggga
    10741 ggcatatgtt tttctgcttt tattgatgaa ttgattgtat tagactcata ataaatggaa
    10801 ttgaaatcca tatcagttga gatttgaatc tcgtattggg ctatatcagc atttcggttc
    10861 caactgaaca ttatgcggtt ctttttaaag aaatcactgc taaaagtttc attgtttaaa
    10921 ggttgaagta gtactggttg tgatattgtt gaagatttat tgatttgaaa tgatcctata
    10981 tcactgacaa ttgtatcatc gggagaaata ctgtaatgag ccttgactag ccagtaataa
    11041 gttccatagg taagggtgtc tgcgtcaatt gatttggttt cggtatcctt tttaattaca
    11101 atgtttttta attcggaatc tgaatagatt tccacagtgt actttgaggg cattgtggat
    11161 gctccccaac tgaaagatat tactggattt gtagaataac tatcaattgt tccattattt
    11221 tcaggaagaa taaggtaggg cttagaatca taaataattg aaaacttgat tactgaactc
    11281 tgtttgtcgc cggaatcatt ttttaccata cagtaatatt ttccggtttc aagtttaagg
    11341 tcaatagatt ttgattttga gttatatgtg ttgtatttgt caaaagagct gttattcgat
    11401 attaaaagta cataaggtcc gtcattcgcg ggatcggtcc aactgaaact gatattggca
    11461 tcgttaatgt ttgaaatata gtatctgttt gaagtaggcg agtctattcg tatcatgttt
    11521 tttattacag ttatctcttc ctcatctact agagcagaat caccagcttt gacgtttttt
    11581 gtgccatcag ctgaacttat tttgacctct ccgtcgtgaa ccgttagttt aatagagccg
    11641 tctttatctt tgtaaaacga agcagagctg ttactggaat caatcacagt tttgtcagat
    11701 tttattctaa gtttactggc ggagtcaacg tttttagtat gaattgaccc ttccaataat
    11761 tcaattgttg taatctcacc gtcaaattca aggtaaacaa gtgtattttc atcaagctca
    11821 atttccgagc ctgaatgaag atttatcgaa acgaaagagt tttcagaagt tttaacctta
    11881 ttcttgttgt atacttcaga attattataa acaatttccc aaaggtcaga tttaaaaagt
    11941 tttctttgaa catcttttac cttgaaagct accgatccga tgggttttgt tatgctttcg
    12001 tttgtaccgg agaatgatcg gtataataaa cctgaagaga taactgcggc tgttaaacaa
    12061 aatagtacta aagcgatttc tttttttctt ctttttattg tacgcacggt tataaccttt
    12121 ggcaacctct aataatcaac taatatacaa gggtatatcc ttgtatgctg ttcttatcag
    12181 aattatttgt tttctatatt actcttgttt gatacgcgcg caattctttt ttgaagctct
    12241 gatatgtttt ttggcgcgtt actgtctttt tttaatccaa gaagagccca taataataat
    12301 ttttgagagc taccctttaa tataatcggc tttacagctt caaaataaaa cttatctttt
    12361 aataatgagt aaatatattc actaacaagg atgtccgtct cataataatc atttaaatgt
    12421 tcaatctgtg aaactttttt gaccgtatca ccaattattc ccaaagtttt attgtaagat
    12481 aggtctccag tgcctgtgac aaatattcca ttgtctatac cgcatctcat ttttaacggt
    12541 ttttttaatt tgtacttggt attaaattta aatatttctt ttcttatcat aagggcacaa
    12601 agagcggcat tgtggcagcc atgttttaca gatttggttg atttaaaaaa aacagtaaat
    12661 acatctccca ggctggcgct cttgactcca tttgtagagc gaattgcgtt attaaatctt
    12721 ttttcacaat tatttattaa ggccagggct gtttctgttt tataaagagt tgccagggag
    12781 gatagtgaac agatattgaa ataggcgagt agtgccttgt catgttttgt ggagaaggat
    12841 gaagttttac ccgattttct gtttttgcta gatcctttat tgattggatt tgacgcgcct
    12901 tttaaaaaaa taaatgagca taaaaccgag agagctatta taataagcat tataagtata
    12961 ttgcggtaaa gaatcatgga tgcagaatcc agaagattat cgcgaagtgc gattgacacg
    13021 gccgcacatt catgttgccc aattcttttg tgtgaaatat aatactcttt ggagttgttg
    13081 tcttgatata atgaaaccct gttgtcagca actgcggtga aaatcttatc tatattatca
    13141 agtaaacctg ttttttttgt gtattcaaga ttttcagttg tttcggctgc tataactgca
    13201 ccttgactat tcactaaaaa cgagtttatt aatccatttg aaggtaattg gccgaaactt
    13261 ggacgtggat caaaagtaat aattacaatt ctatccggat tttcctgaag atctgtagag
    13321 attgccagtg atagtgccgg tgttccaagt tgttcagaaa aatcagttat taaagttcta
    13381 tttttagtta cgtaaagaat tcttttaaag ttgatattga cagcatctgc gatttttttc
    13441 agacttacca ttccactgga ttcataaata ggatttattg atgtttttgg ggttttatta
    13501 tcataatcgg ttatagttat cgaaataatt gagctactga tattaaacag ttcatttgaa
    13561 ctggaaaaat ccgagtcaat gtactttgca acagattcaa aagattcaaa agaggtctga
    13621 aatgatgagg ctatcaaaga agagcggagg agcagttctt cagttactct gttatctagt
    13681 tctgatttat aaaatgatga acttagaaaa agtaatgata gtaaagctgt tataagtaca
    13741 aggcttataa taaaaagaag tttgaattga gatatatgtc tcattttgtc cttcgtagca
    13801 ataattgaaa ttgaggatag ctctcttttg tgaataacgg aaaaaaatca ttttagattt
    13861 tttgcaggtt aaataacaaa attagctcca aatttgtata ttatgatcag tttatagatt
    13921 attgtcaacc atacttcgaa aaacagttgt gcaataagca tatattgaag atttgtatgc
    13981 tggtaggctt gtatcgatga ggtagaagcc taaagtaatt aataagaggt atattatgga
    14041 ttatccggaa tttgatctgc taactactgt tgaaacaggc gggtgttccg ctaagcttcc
    14101 tgccggagca ttaaaagagc ttcttgctgg tattcctcag ttgaagtctg agaaattgat
    14161 aataggtaat gattgcagtg atgatgcact tgtcttcaaa attaacgatt caactgcatt
    14221 aatacagaca acagatttct tcccaccact ttgctcggat ccatatacat ttggctatat
    14281 agccgctgtt aacgccttga gtgacgtgtt tgcaatgggg ggagaggcgc taaccgcact
    14341 taatatagtt atgttcccta cagcttcaat agataaatcg gtgttaaaat ctattcttac
    14401 tggtggtgca gacgcggctg ccgaggctgg cgctgtactg gctggcggtc ataccattga
    14461 tgggcctgtg ccggtttacg ggttgtcagt aacaggatct gttcatcccg acaggattat
    14521 gagaaacagt ggcgcaaaac cgggcgattt acttatttta actaaaggga ttggtactgg
    14581 agctttaaca gcagctttgc gcaccggaga aatagtagct gaagattaca gtgacgcact
    14641 tttgtcaatg aaacagctta atatggttgc ttcacgtatc ttgcaagaat ttgatgtttg
    14701 tgccgcgacc gacataacag gattcagcct cgctgggcat gctcttgaaa tggcattggc
    14761 ttcaaaagtt catttaaaaa tgtatagcga agagattcct cttcttgata atgcttatcg
    14821 agtgaccgaa actggctgta tcccttgcgc gtcgtttaga aatttgaaat ttgttgaaga
    14881 tcgtgtgcaa ttctctgaca aagttgatta caaccgaaaa atgctttctg tagatgccca
    14941 gacttccggc gggctacttg taagtatcaa tgccgataag gcaacagatc ttaacaatgc
    15001 tctgctggca gctggatatg attattcaac aataattgga gaggtctttg aatcaggtta
    15061 tgggaatact ccgatagagg tagtataaat ggcaatttat ttagaacctg agccgtttct
    15121 tgaaaagtcg gctttcttga aaattattga tgtaaggtca ccgggtgaat tcgaagccgg
    15181 acatatccca gatgctgtta acattgcgct ttttgataat gaagagcggg cagtggtagg
    15241 tacaatttac aaacagcagg gtcgtgagct ggctgtgtta aaagcattag aaattgttgg
    15301 cccaaaactt gttggttatg tgacagaagc tcaaaaatat gtggtaaatg gcgaaatcct
    15361 cgtacattgt tggcgagggg gaatgcgcag tgagagtttt gcctggtttt gtgaaactat
    15421 aggattaaag gtctatctgc ttaaaggcgg gtataagtct tataggcatt atattcgtca
    15481 aagtttttta ttatgtgata gactggtagt tctgggaggt tttacaggtt cgggaaaaac
    15541 tgagatatta aatgagcttg gaaaatcggg agagcaggtt atcgatcttg aaggattcgc
    15601 gaatcacaag ggatcggcat ttggagccat cggacaaaaa gagcagccta caaccgaaca
    15661 gttcgaaaac aatttggcac aagagtggct taaatttgat atgcgaaaac ccatttttat
    15721 cgaagatgaa agccttaaaa taggtacagt tgttattaat gaaaatctat attccaggat
    15781 gcgctccaca aaactttatg tcgtagatat accccgaagt gaaaggataa agcgtatcga
    15841 gcgtgactat attattgttc ctgccgaaaa gcttgtttca atagttaaaa gaatcgaacg
    15901 acgtctggga ggagaccggt gccgtgagtg ttgtgaactg tttgtatcgg gagattataa
    15961 gggcgcaatt gatatccttc tggtatatta cgataagggc tactctcatg gaatgtccaa
    16021 acgtaatcag gatatggtac attccgtaaa acttgaatcc ggagatcccg ttaaagcagc
    16081 aaaggcatta aaagatgctg tttataatgg ttaaaacctg gattttttat accgccatag
    16141 agcgcattaa ctcatcactg tttataaaat tgattatttc ttctaacgcg ccgggttgcg
    16201 aaagtatggt ataaaagttt tccttgattg aaggggcatg acgtaccgct gtgattagtt
    16261 cttccctgtt aaggggattt tcggcggcga atttatcaaa accacagctt tccatagatt
    16321 ttcgtatttc attgtgtgtc tctttttgta caaagctaac agcatacgcg gcaatgcctg
    16381 tttgtatccc atgtaaagaa ggtgatttag atattttgtc ataagcgtgt gatataaggt
    16441 gttcgctacc actcgccgga cggctgattc ccgctatttc cattgcgata cctgtcatca
    16501 taagagaaga accgattgtc ctgacataat ccggatttga aagatctttg tccgggaaat
    16561 tgataaaagc gtcaagtgct gttctaacaa ttacaaccga aaagtcatta acatattcgc
    16621 cagttttttt atatgaaagt ttccagtcaa aaatggctgt tgttttgcat agcagatcac
    16681 ccatgccgga gaaaatatat ttttggggag cattttgtaa aatatccgtg tcaatgatta
    16741 cactgtatgg caaatttgta ctgatggatc tttttttatt ccgtacgtaa agtgaagcaa
    16801 caggagaaca aaaactgtcg ttagatataa tagtaggaac agtaaatagc ttttttttgg
    16861 tgataaacga gatatatttg cagtaatcta tcacctgacc gcctccaatt gccacaattg
    16921 ctgaggttgc tgaggggata tccaagctgt tttcaaagat ctcttcgatt gaagttgtat
    16981 taacattttt ttcatgtaat actgtaatac cggaagattg cagagaaact tcaactgtgc
    17041 tattgaatag ctgtttgaga ccgtcacccc agaaaagggc aatctctgaa cagtttgatg
    17101 tacgcaaata tttaccaagt ctttgtaaag cctggggttt aattctaaca agttctggaa
    17161 caacgatttc catgttttct ccgttttatc gatataggtt acaagttatc tggagtctct
    17221 ttttcaaaca gattcagatc aatttctccg acattattaa aaatatatgt aggtttataa
    17281 gggaatgtgt ctaatgtgct tattgtagaa acaccagata atacaagaca ggttcgcatg
    17341 cccgcttcaa gaccgctaat aatatcggtg tccattctgt caccgatcat tactgtttct
    17401 gccgagtgaa caccgagcat ctgtttggct accatcatca taagagcatt aggtttgcct
    17461 acaatatatg gagccttgcc agtagcggcc tgaatcgcgg cgataatagt accgcatgcc
    17521 ggttctatac cattttcaac aggatcaata atgtctggat tggtggcgat aaatttagca
    17581 cccgcattaa taaggttaat agctcttcga agcatatcaa agttcaggct tttggtttta
    17641 ccaacaataa cataatctgg atttgtctcg gtaagtgagt aacctgcttt atatagctca
    17701 ttagaaagag cggctccgcc aataatatac gcggtaccag attttttttg actttttaaa
    17761 aaagtagccg ttgccattgc tgatgtgata aagttgtctt ccgtaagacc ttcaataccc
    17821 atgttcttta gttttaatac cagatcgatc tgcgtctgct ccgagttatt ggttaagaac
    17881 aagaatggaa tattcttctc ttttagggca gagacaaact ctttggcgcc gtcaacaaga
    17941 ttgcctcctc tgtatattac tccatccata tccgaaatta ttgctaatga catagaaaac
    18001 tccatttatt ttttaaatca gctaatcctt gttataaagg tttaaacatt aataagagtc
    18061 aattagaaaa aatctaaaaa ttaaccgaca aatttcgaaa aatattgggt ttcgcaaaaa
    18121 aatgattatt ttctagttca gtttaaggtt gttacaaaaa gatatgaatt aaaaaccaag
    18181 aaagaagtag attaatatat aaaaaagtgt tagaaaagga tcatataaca tatttagaat
    18241 tggtttatga gatgtatcat tacgaaaatg agactgttta aagaactact tacaaaccgt
    18301 tttatcttca atattggtta ttagtccgga atagatattt atccggttat ataaattaca
    18361 aggagactta aatggaaaca agattctatt tcagaacctt tcacatgatt gcaattattg
    18421 ttctgattac tgcttttttt ggtggatgta caaaaaaatc ggatcttcga aaggctgccg
    18481 aaaaacaagg tatccttata ggtacagctg tcgatacaag agcgcttgaa gatgagaagt
    18541 attataaaac actggtacag aatttcaatg tagtaactgc agaaaacgta atgaaatggg
    18601 aaaccattca tccccgaaga gatgtttacg attttactga aggagataaa cttctggctt
    18661 tcgcaaaaaa acatggtatg aaaatgcgtg gccatacact tgtttggcac aatcagaatc
    18721 ctaattggct tttgtctcaa ccatggaagg cagatgagct atataaggtt ttggaagagc
    18781 atattaaaac tgttgtaact cattataaag gtgaagttta tgcctgggat gtcgttaacg
    18841 aggcactcga tgttgatgga taccgtgaaa cattatggta tctctatcta gggcctgaat
    18901 atatcgagaa ggcattcaga tgggctcatg aagcagaccc ggacgctctt ctctattata
    18961 acgattatag taacggtgaa ataaacgaca aatccaacta tatgtataat atgcttaagg
    19021 atcttcaggc agacggtgtt ccggtgcatg gagttggtct tcagatgcat ttgagtctcg
    19081 agttcggttt tgactttgaa agcctgtaca tgaatgtgaa tcgtctgcat gagctaggtc
    19141 tccatgtcga ttataccgaa attgatgttc gtatcaaaac tcccgcatcc gagtctgatt
    19201 acagagagca ggcaatgagg tacaaaaaaa tgatggaaat catgctcgct tttgataact
    19261 cgtatgcata tactgtctgg ggagttagtg atgctcactc atgggtgcct agcgcgtttc
    19321 gcggaacagg tgatgctctt ctttttgata aggagataga gcccaaacag gcttatcata
    19381 tgcttgtcga aacacttgaa aaagggcctg tggagcttaa ttacagcaaa aattatgagg
    19441 acaagtttag aagccgaaaa gcaattcctc cttttagagc tcacgccgta gcgtctgcac
    19501 cgaaaattga tggcaaccca aatgacaagg cttgggaaaa aggtgttact tatcctttta
    19561 actataatca gcttaatctt tatgatcagc gaattcctga cagcaatgac ctttggggtg
    19621 aatggacggt tgtatacaag ggaaatacaa tatatggcat aattcgccgt aatgatgaaa
    19681 agactcgtac cgatcataag cttgattacg agaatgataa tattgaagtc ttttttcatt
    19741 taggtgatgt atttgcacag ctgcggtcga gagtcgggca tgattgggag tctcacgctt
    19801 atgatggcga acgaaaaata gcttggaata aagagggtac agtatgtgaa tattctgtaa
    19861 ctcttcctat agatgactta acaggtatga tttcaggatg gaacatttct ctttcagata
    19921 atgacggaaa ggagaatcgt gatcatcaat tgtatccgat taatggacaa aacgatggat
    19981 ggcaggggaa aaatttggga acaatgcaat atgttgggga ttctcctcga gttcccgaag
    20041 atccacgcgt tgttatgcct tttatagcgg cacctgcgtc atctgagcca gtgcttgacg
    20101 gatcattttc ggatgatgag tggattgatg ctgttaagta tcagtttgcc tacaatcaga
    20161 ttaatccaaa aaaacagagc ataggtctta aaaatagggc aatatctggg tcttggggaa
    20221 tcgtaagaaa agggaattat ctttacggtt ttgttttgcg caacgacgct aaaactgtaa
    20281 cttctaatgc ggatccggct ttaaatgaca atgttgccat ttatcttgaa attgaagatg
    20341 gaaatgctgt ggtcatgcgt acagttgttg gtaaggattg ggaagaatca ggattaaatg
    20401 gtgaacatca ggcgaagtgg agcgatgacg gttcagtact tgagtttagg attgaattgc
    20461 cttcgaatgt tgcttcaaga aaaatgattg gctttaatat cgcgctttct gataatgatg
    20521 gtggtgataa actcgactat aagctatatc caataaccgg tgacgacaag agccaagagg
    20581 ctaaaggact tgccgagttg gagctacaac tctaaatagc tcataataat tttaggtaac
    20641 ttctataaaa ggatcccggc taataaaccg ggatctgtta ttttaaaaat aaaatctttt
    20701 gataactatt tactcggcat agacatacat gaatccggaa gagctgtcag gtttaatgcc
    20761 tttacgctca aaaagctcac tcaaggtaac aaattcataa ccttcacgtt taagtttcgg
    20821 aataagagtc tcaatagctt caggtgttgg atgcggtttt ggttgaacat catgaagaag
    20881 gattatagcg ccatcgcgca tgccactcat aacatgatcg ataatttttt caggtgtgtc
    20941 gccaccaccg cctggccagt caaaaccgag cactccactt gcaaatggca aatctataga
    21001 ttcatacatt gttttacttg cggatagatt tggtgctctg aaaaattttg gtttaactcc
    21061 ggcatactct tcaatgatat ctgatgtttt tttaacataa gttttcacct gttgtgcgct
    21121 catgtcttgc atcccatcat atgtccatga atgattacca atttcgtgac cggattttga
    21181 tattttcatg acaatttcac gtgttgaatc attaaaattt tgaccaacaa ggaagaatgt
    21241 ggcttttaca ctgttttttt cgagcgtatc aagtactcga gtagtaagct ccggatcatt
    21301 acttggaccg tcgtcaaatg ttagagcaca gatcttattg gcagtcggat acggctcacc
    21361 agtaagtggg tcaatatcct ttttagaaca tgaagtacaa attgttgtaa ttaataaaag
    21421 aaaaattgtt ttattcatga ttcctccgat tttttttcaa atgattactc gattacaaat
    21481 tttttttcta tctctttttt ggataattag cactgttttt tttatatgat agaattttac
    21541 cagttgtaga aattattaat taccggtttg atatttttgg cggtattcct tgggagaaag
    21601 tgattcctgc atcttgaaaa gtttattaaa atgagctatt gaattgtacc caatagcaaa
    21661 tgcaatatca ataatcttac tatcactatt aataagcaaa ttttttgctt catcaacgcg
    21721 tatcagatta atatattgct taaatgaaag attcgtatac ttattaatta tttttggtat
    21781 tttgtaaggt gataagttga gagcttccga tatttgctga accgaaatgt atggatcatt
    21841 gtaattagct gcaagatatt cgaagagttt acccgcttcg cgttcttttt cattaccaag
    21901 gttaagattt gaatgtattc tgataatgtc ctgcgcatat ttaacagctt tgtgtgattt
    21961 tagaatatga gataaaaata atcccaataa tgccaaaacg gaaataatta acatggataa
    22021 tatggaatag taaaatatat cttttttaaa tccaaaatat gttatactta taccaagtgg
    22081 agtgttcagt tcatcaatag agctattcag tatttcaatt gaccaaagag gattcttgcc
    22141 aatcgaatca atgctctttt tctcgatttt atgtttaaca agaaaccatt ctggagttaa
    22201 gaagtcgcta agaggaactc taattgtcga aaattctggg gtaatgtcaa ttatggaagt
    22261 ataataatag tatgatattt tatgagggta gcttgtgttt tttttatcag agagattgac
    22321 ttttgtgatg atattaattt ttaaagaagt ttttttagat gtttttgcag tgatttcgaa
    22381 aaaatcaaat ccagatgaat atagaggtac agaagaaaga atttcgatgc cgacacctgc
    22441 atagggaaat gcaaagccat ctcgaagaat atattggaga tcaatctgac cgttgttcgt
    22501 gtttatcgag attatttcag aatttccaga gtcattatgg tcagtaattg ggtaaattct
    22561 cgtatcggtt tcaggaaaaa tattacgact cgcggtaaat attaaatagg ctatacttat
    22621 ggagatagaa agaaagatga aaagcaataa aataagtatt ttgtttttgt tcattttata
    22681 ggatgccatt tttaaaaaaa ttatttggta gtgataacag caaccattat cccaatacga
    22741 cttttatcag atacgagata atcatgtaag tttttttttg aaatcacaac ttttttatgt
    22801 gagaaagaag cgtgttgaaa cataagttta ccattaatga atcgagcaaa accgacatgc
    22861 gaaataaaaa ttgatctttt acttgttaca atagctatta ttgcaccatc aggtataaga
    22921 tgggatttct gtttaacctc tattgttttt aaataataaa aggttttttt tgacaggttc
    22981 gcttcaatat ttctaatttt agctagctgt tttttttctt ttaacatcgg gtatcgttta
    23041 tctgaactac tcatcgcgga tattattctt gtatcgggtt ttccaccaag ttttttggtt
    23101 atgtttgata taaatcctct acgttcattt tcatgtagcc agtcactaaa atagtgcaaa
    23161 cgtgatgagt atccatcaat aataccgttt cgatatctta tatttgttag gtggttacag
    23221 aaagattcat aagaatgatc tgttgttgca aacattagtg ctaaagcggt aactgtttca
    23281 acataggtaa cacaatctaa ggcgtcaagc cttacaatta atttttcagt ttcgttattc
    23341 tcaagggtat gcggaacata cggagttccc gtaaagtaat ccattagaaa tgttagagaa
    23401 gttgatctac ctgtactact ttgcatgatt ctattaaaaa gagtacgttg cccagatctt
    23461 tccctggact gtagttcaat attatcagct gagcagttta ctgaagtcgg cataataagc
    23521 ataagcaaga ttaaagcaga aaacacagga aaagagctgt aaatatcttt caaacaacca
    23581 aggagccgaa taattccaag tttaactact ttaaataacg ataaggtgac catgaggtaa
    23641 tatttattac tggaacggta tttagtcaac aaatatgaag attaaatgtt attttaaaag
    23701 atttttttaa aaaaaaagat ccacttttat aaagcggatc tttatgctcc cccggcaaga
    23761 ctcgaacttg cgacaaatag attaacagtc tactgctcta ccaactgagc tacaggggaa
    23821 tatgaactta caaaagttaa tcagtaagta tggtggatat atataaatgg ctgtgtatac
    23881 tgtcaagtaa attcggaaaa ataatattga tttttttatc ggttaccaat aaaaaatttt
    23941 gtaaacatct tgccacaatc gtatgataat agaagtaata cggtaaaaga ttcttttagg
    24001 agtaattatg gatacacttg aactgcccga ttataaccgg gaagaagagg aggggatttc
    24061 ggatcagaaa ctggaagaac tccggaaaaa aacctttact ccggatttaa ctggtctgat
    24121 atcccgtgtt acatctcata atgaagatca atccgtaaca accgattcag agatcgaatc
    24181 agtttcgaca gatgacaggt tcaatctgtt aaaaa
//
LOCUS       DAOTGZ010000014        23490 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_3064
            NODE_2540_length_23490_cov_5.372818, whole genome shotgun sequence.
ACCESSION   DAOTGZ010000014 DAOTGZ010000000
VERSION     DAOTGZ010000014.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868088
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 23490)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 23490)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:22:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,785
            CDSs (total)                      :: 2,751
            Genes (coding)                    :: 2,732
            CDSs (with protein)               :: 2,732
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 12 of 19
            Pseudo Genes (internal stop)      :: 5 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23490
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2540_length_23490_cov_5.372818"
                     /isolate="AMR_MDS_3064"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..573)
                     /locus_tag="P5123_02065"
     CDS             complement(<1..573)
                     /locus_tag="P5123_02065"
                     /inference="COORDINATES: protein motif:HMM:NF017508.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trigger factor family protein"
                     /protein_id="HRX15080.1"
                     /translation="MNILSEKKHENAIVELEIEIPVEKVELEYKSVFEKIRKNAKVDG
                     FRKGKAPVQMIETKFREYADEEVAENLAKSTVFDAVKEKELMPIMEPKIHYDRVLRNE
                     PFKYKAFFELMPTVELGEYKGLKVDEAACEITELDVQNEIDISRERTATTEVLSDPET
                     AVENGHLVKFQLKRIDNVEPGQIDSVEFREY"
     gene            complement(905..976)
                     /locus_tag="P5123_02070"
     tRNA            complement(905..976)
                     /locus_tag="P5123_02070"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(942..944),aa:Gly,seq:gcc)
     gene            complement(980..1061)
                     /locus_tag="P5123_02075"
     tRNA            complement(980..1061)
                     /locus_tag="P5123_02075"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1025..1027),aa:Leu,seq:tag)
     gene            complement(1178..1597)
                     /locus_tag="P5123_02080"
     CDS             complement(1178..1597)
                     /locus_tag="P5123_02080"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRX15081.1"
                     /translation="MKIVHILKKYLVLRLFCLVILLFFCLPTLIVSEDFEKDFRLTTV
                     KKDFETYRDRDVSFVLRLQYCDLNYNEISFYDMENINLRFSIKDYQENPVLKRKLRNI
                     SRGLYYRVKCKLIEMDEDGTISADLIDFTPYRLEQLP"
     gene            2119..3624
                     /locus_tag="P5123_02085"
     CDS             2119..3624
                     /locus_tag="P5123_02085"
                     /inference="COORDINATES: protein motif:HMM:NF014600.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine carboxypeptidase family
                     protein"
                     /protein_id="HRX15082.1"
                     /translation="MKKILIFTLSFILVSTISEYAFGETARRYMIITNSVFCKTAPSS
                     DSTDMEADIGFGSVILGEKVIQNETDFDYIKLSGKEDCYVPLEMTIEVPLVSFSSEKG
                     ALSSENGKDLPIFFVPNDLMPINKMYSFGDNRVLAKKSILKDLYAMLSDAKKNGHKFY
                     VANGFKDLQVRIKEYEEAIRLNPKQTLVPKPVHSSSATGLVIDLTDNVTGSNFEYELK
                     NDQAWKWIYENSSRYGFRTSGKSFRFEYSGEKSRRKPKTDKIFDEAINKRIEIPSLCT
                     VHVLKNSTNKRASFIWVHHNEKSAGKTLKYALSKYGGHSIYIINGDKRNVYIPYNRRS
                     YFIDPNRIYTPKGIEKYLDRHHRKLNKNGRRSVYKKAEKLKDTILSSIDWTEKKYLIA
                     LHSSRPGSDFSIEEFNAKNSYLTYTNPDHNPKNLFFTTSLQDYYFFKSRGYNVVYQRS
                     IEDDGSLSIYAAKNNLPYINIEVVEGDDKFQQKMLDLVIECVNQHFKEKSL"
     gene            3684..5345
                     /gene="ilvD"
                     /locus_tag="P5123_02090"
     CDS             3684..5345
                     /gene="ilvD"
                     /locus_tag="P5123_02090"
                     /EC_number="4.2.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013176307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004160 - dihydroxy-acid dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009097 - isoleucine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009099 - valine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxy-acid dehydratase"
                     /protein_id="HRX15083.1"
                     /translation="MRSDQFKTGIGKAPHRSLLKASGLSDEEIRRPIIGIANSANEIV
                     PGHIHLDTIVEAVKKGILAAGGTPLEFTTIGVCDGLAMNHIGMKYSLGSRELIADSIE
                     IMAMAQPFDGLVIVPNCDKIVPGMIIAAARLNIPTIMISGGPMLPGKYVGESVGLDNV
                     FEAVGKAAKGDMTEEQLYSLECSACPGVGSCAGLYTANSMNCLSEILGISLPYNGSIP
                     AIHADRIRLAKKAGAQIMELVDKQIKPRDLITKDSIYNALAVDMAIGGSSNTALHIPA
                     ISHYAECPITLKDINQVAEKVPHLCSLAPGGTDYIVDLHRAGGIPSVVNELMKHGLIR
                     ENCMTVTGKTIGENCKNSPIKDTSVIRTFEKPVHKDGGLAVLTGNIAPDGAIVKKGAV
                     DPSMLKHSGPAKVFHSEEDAVDAIMTGKIVKGDVVVILYEGPKGGPGMREMLTPTATI
                     AGMGLDKEVALITDGRFSGATKGASIGHISPEAASRGPIAAVRTGDIIEIDIDNNSLN
                     VKLSDEEIQKRIAELPEYVSPIKRGYMNRYSKLVSSADKGAVFEL"
     gene            complement(5585..6226)
                     /locus_tag="P5123_02095"
     CDS             complement(5585..6226)
                     /locus_tag="P5123_02095"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRX15084.1"
                     /translation="MEKNILKSIKDELESNFHQPADFLFYYGFLSAVATNPYMIEQKI
                     WIKYLIKRGLGSWDKKNRDNVAGFKNFLVKTAKELSLAIEGKKFSLHIEMWSDYCEKG
                     LTFASGFWEGMCLWTDFDEYSNNNRFHLLLMPINTLIFPEFYAKYYAVRREDISAVVQ
                     AALRELPRRLYSLKDYLKKGKRNKSLIFKRVIRKSKSVRMAIFPFNCDIKPLS"
     gene            6704..7093
                     /locus_tag="P5123_02100"
     CDS             6704..7093
                     /locus_tag="P5123_02100"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HRX15085.1"
                     /translation="MNKTQNIKKVLLLDDQQMILTTLSRMIEILGGTVTTSTDGKDTL
                     ELYKNSFEKNDPFDLLILDISIDNGIGADIIIDDLLKIDPDVKAIVSSGDPNHDAVIN
                     YKERGFASVLLKPFTIDKLKDILDGLD"
     gene            complement(7118..9202)
                     /locus_tag="P5123_02105"
     CDS             complement(7118..9202)
                     /locus_tag="P5123_02105"
                     /inference="COORDINATES: protein motif:HMM:NF012869.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HRX15086.1"
                     /translation="MTNIQLYKKVAFFVRPYRFFFIGALFLSIVAVCAELVVPVLTRQ
                     AIDKHIGRRYYYITKKDAALFETNNLSNEIKMFSDGSLLVDYGSISELSKKNRQIIAE
                     NKDLFELFYLVSSDKYSGPLLETMIKKEGYYLVPYRNLVSLNRDAIRQLRAHDIESVV
                     IIFMIIIGLTVVGFITAYAELLLVSYSSNHVIHDIRMALYKKVVNLPVRYFEEHKTGK
                     IVTRISNDIENIQEFFAAVFVNIIKDIAIITGIVAVLIVINLRLGLVTMAMLPVAAFI
                     SAIFRKKMRRSFTRVREALAVVNGRLSETLSGIAVIQIFKQQSAFLNVFNKESKNYRN
                     ASSRQVFINSVFTPMISMLRYCGMGYIIYLGSNDVARGFITLGTLVVFIAYLEKFFRP
                     IQDIAEKIQIVQSALASADRVFKLMDQEPEYDCSQSGSCRELVAKKSEYFIEFCNVWF
                     AYREEDWVLRDFSMKISHGEVVAIVGETGCGKSTIIKLLSRMYMIQKGAIYINGININ
                     SISKKSLRRLIGVVQQNVTLFSDTVRYNILLGREPAGGRSFEDVCNYASCTSFIEKLP
                     YKADTILSEEGKSLSFGERQLISFARIVAYDPAIFILDEATSNIDTETEHAIQKALNR
                     ILKEKSSIVIAHRLSTIRHSDKIVVISNGSITESGSHNDLMSKKGRYYRLYMIQYKEH
                     LEEDDIKSLEVK"
     gene            complement(9199..10926)
                     /locus_tag="P5123_02110"
     CDS             complement(9199..10926)
                     /locus_tag="P5123_02110"
                     /inference="COORDINATES: protein motif:HMM:NF012869.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HRX15087.1"
                     /translation="MTLFRRPLYYLLKRRYYFAAGIISLLLVDIFQLFIPRAVKKMID
                     DLFALDTSRIHIWFSHILLLALGMAIFRFFWRMLLVRTSFYIEEQLRNDLYKKYLYMD
                     QPFFDRRSTGSLIALATNDLQAIRMMFSMGVVSACDGIFLTILSVVFMFSMNSRLTMY
                     VLIPLPVITFFLIIAGKKIFGGFTRVQEYFSDLTGRLQEYVSSIVLIKNFTLENESIR
                     SVEKSSVKICDQNFSLMRVWGALFPLMALVTGIAAAFAGIRGGTMTIMGELSLGEFTA
                     FFSYISILAWPMMAIGWVMNLFQRGRASMKRINDALDANAVVRDKPLIAEPQYLPKPT
                     FELKDVSFTYPEALHPALKNISMIIPYGCRIGITGKTGSGKSTLADLLLRRYDPSTGS
                     VLLNGQNYRDLPVKYILSHIKSSPQQNTLFADTLRQNIVFGSSNDKEEQLRIACSDAK
                     LSDTVNDLPKKLDTMLGEKGVNLSGGQKQRVALARLLLSPAPLCLIDDSLSALDSETE
                     KSVYNSLLKIPGSMVIISHRVSAISNCDIIYVLDEGTIAEKGTHEELVKQKGLYSDIY
                     TRQLVQNRL"
     gene            complement(11053..12297)
                     /locus_tag="P5123_02115"
     CDS             complement(11053..12297)
                     /locus_tag="P5123_02115"
                     /inference="COORDINATES: protein motif:HMM:NF024853.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin domain-containing protein"
                     /protein_id="HRX15088.1"
                     /translation="MNKTYILLSIFSFVGLILAVILLADHYIPQKTAEFLACGDGLQG
                     SCSELSSSPYSMFFGIPIASYGFFFYLWIFLSLHLFRESGERYWKTFMYLMIPISVAG
                     IFFDIVLGALLVYLQVFCQYCVMTYVVNIAILAVVILAYRKMRASGDMSFKEALFGFA
                     KISGKNDDRKFSTGTFVYFTAILFIFVVAFSLYLEERSSEGSELNKKLTGYVEKYKEK
                     EILEIDYPETGFVVGKKDAVLTVHVFTDPFCPACRNFNMIEKVLVKKYKGKINFKQYY
                     YPLNSDCDPAKSGIVINGICAVTRLFYAADMLNIYPKIFEKFYEKFDEVSELSSSGAH
                     IDKIIEIVAESDEQKKALIEMFNSDKPSERIIKHLDLAKKLKISATPTLIINGRLMKG
                     YPKDKFMDAILRYELSESIKNN"
     gene            12609..13463
                     /locus_tag="P5123_02120"
     CDS             12609..13463
                     /locus_tag="P5123_02120"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013672.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HRX15089.1"
                     /translation="MKLSFFKTFKELAINTSILAIFFVFICTITLILYLRVSTLGEND
                     DPGFITKNPNEYREIDAIPILLYHDIDGNGPYSITKEQLRKHFEFFRLNNIDVISLKE
                     LIRLQHENRSPENKSVVITVDDGYISAYTKILPLMQEFDYPVTLFIYTNFIFEKSDKH
                     MTWTMLKEMEKYGFDIQSHTVSHADLVKLIGKGDTESLNSIEYELTQSKKIIEDKLDK
                     EVTLLAFPYGYYTPKLLDMARNSGYIKTFSTDYGANISLHSNYCYRRHHIKKDFNETN
                     LKKIIQMR"
     gene            13539..14285
                     /locus_tag="P5123_02125"
     CDS             13539..14285
                     /locus_tag="P5123_02125"
                     /inference="COORDINATES: protein motif:HMM:TIGR01856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004401 - histidinol-phosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol-phosphatase HisJ family protein"
                     /protein_id="HRX15090.1"
                     /translation="MIKYDLHTHSQFSPDAEDTVDLLCLTAIKKGITHLAITDHFDAH
                     KDDTYPFYYNYEKQSIAIDNARVKYKDKLTLLKGIEYSQPYNTPEIFAQTIKLEFDII
                     IGSIHKIGHYNDYDKDNDTKYKKYFTNIKRMIEHGGFDVLGHIDFPARYYSGYYSHSR
                     ELEDAVKALCKSEVIPEINSSPLRRGMPFAMPDELILQMYTDFGGRYITTASDAHNAN
                     DIGADFDYIQKLIKRFNLLPVYFKDRKMFE"
     gene            14443..15678
                     /locus_tag="P5123_02130"
     CDS             14443..15678
                     /locus_tag="P5123_02130"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HRX15091.1"
                     /translation="MITEKKILIAEDDESSAELLRAVCEEHGYTTDIASNGKDAYKMY
                     RQDNYPLVITDLEMPDMSGHDLIKNIKKINDRQVIIVSTMHADSETIISVMREGVYDY
                     IIKPINLTIIIQQIKRAFEADELYRSRELIEQERIIKLENQLEWVQWNKEIINRDNDR
                     IDTALFDSLNNSFNQGAGFGALLSLINLVASSIKKEGDHYVVPSDIFDLIKSNAAIAE
                     KTLNFFSDLSLLMKNDISISNSTCMDIYDLLLEIVNSDNIKNLSNLRGHTILLSNIPA
                     DIKDTVCKINPRYISTAINELFVNALKFSTSNSKITVMLRYSGGKFLISVLNTPQPTK
                     SDIIGIPPEYQNVVFEPFFRLVKVVYEDYGTLDYGLGLTLVDKIVRSHGGRVDIGNVI
                     DHTDPSRDPVTKVLAQIVL"
     gene            complement(15754..16791)
                     /gene="ilvC"
                     /locus_tag="P5123_02135"
                     /pseudo
     CDS             complement(15754..16791)
                     /gene="ilvC"
                     /locus_tag="P5123_02135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011134143.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ketol-acid reductoisomerase"
     gene            17032..18150
                     /locus_tag="P5123_02140"
     CDS             17032..18150
                     /locus_tag="P5123_02140"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRX15092.1"
                     /translation="MDRELRKHFLNLLIYSSLNTQQLNYLGEQIDNSFNLYRESGFGD
                     TIPIPRQSAAKILVNYFTAEEDIVNLFTTLLANQGKRFYNSELRLNGYEDFIKFLAKK
                     KWIFDNQVFQFFRDPFFENEINVLKSIRIIDLRKKDEIDSLLKKIEDVTQTLGDKDLE
                     WSVNLRLYDLDREISQLIRKIIGMLLIRQNLQSITFDIFTCLKELAINASKANYKQLF
                     DKLVTKPEGIDANKNYSLFLKRFKEEIAENGNRNLIKLAQQKDRYINITFQSTNQGIG
                     VWVTNSQNITAVEKKAILKKLGYKKTDIFAYADDKNSEGAGLGIGLVLSLLRIYSDED
                     IPLKVVFYPNFIKIGFFLDRSLTAARLQEKLETQKEQN"
     gene            18576..19763
                     /locus_tag="P5123_02145"
     CDS             18576..19763
                     /locus_tag="P5123_02145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020611744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="homocysteine biosynthesis protein"
                     /protein_id="HRX15093.1"
                     /translation="MKTYEEINRKIASKTAVVMNAEEIIDYVEKKGLDKAAEEVDVVT
                     TATFGPMCSSGCFLNFGHSSPKIRITEAWLDDVLVYSGIAAVDAFLGATQLRHNDPAN
                     MYYPGDFRFGGAHVIEKLVRGEKVQLFALSYGTDEYPCKELRTYITIDDLNQAQMVNP
                     RNCYQNYNIAVNCSDRSIYTYFGHLKPNMQNLCYCSAGQLSPLLNDPYYKTIGIGTRI
                     WLAGSHGHIYAEGTQHSPDCLRSENGVPREGAGTLSLTADMKKMDPEYVRAVSLKGYG
                     VSLSLGVGVPIPILNKEILKSTTVRDRDIYAPLIDYSSDYPLATGRILTEYNYESLKS
                     GEVEYNGKKIEVGSISSYSKALKIANRLKEEISVGSFTLAEPHTPLPVTGSMNRMKIQ
                     QER"
     gene            19764..20192
                     /locus_tag="P5123_02150"
     CDS             19764..20192
                     /locus_tag="P5123_02150"
                     /inference="COORDINATES: protein motif:HMM:NF020940.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HRX15094.1"
                     /translation="MITRKYLLKFPEKAVGKPLIYHLVKDYDLTVNIYRAKIDPDDIG
                     YMVLDVSGRESDIERAKIFFDENEIIVTETMNGMRWDKEKCVSCGTCITHCPSNALYI
                     VDRKTMEVSFNNEKCIECMNCISHCPFKACSSIFSSSISE"
     gene            20189..20932
                     /locus_tag="P5123_02155"
     CDS             20189..20932
                     /locus_tag="P5123_02155"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="UPF0280 family protein"
                     /protein_id="HRX15095.1"
                     /translation="MTPEENKFTYKDSNFTIHCSFFSEAKDEIISQRTILEKYLEKNP
                     NFKSSLTPLTPTRDAPLIVKTMCAAAEICNTGPMAAVAGTIAQFAWQKVFDIATNDKI
                     IIENGGDIYLSSNSPIYIGIFAGKSILSGKLALKITPEETPLGICSSSGTMGHSLSMG
                     NCDLATVFAKNNAIADCAATMAANIVKTVDDLEKAADYLNSINEVRGGLIIKDDRIVI
                     VGETPEIIKNSDKKLTTKITKAEGSIFSF"
     gene            complement(21012..21299)
                     /gene="gatC"
                     /locus_tag="P5123_02160"
     CDS             complement(21012..21299)
                     /gene="gatC"
                     /locus_tag="P5123_02160"
                     /inference="COORDINATES: protein motif:HMM:TIGR00135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030956 - glutamyl-tRNA(Gln)
                     amidotransferase complex [Evidence IEA];
                     GO_function: GO:0050567 - glutaminyl-tRNA synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0006424 - glutamyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatC"
                     /protein_id="HRX15096.1"
                     /translation="MSIDISIVERTAELSRLHLDEDEKKQFVSQISDILEYVEKINTL
                     DTSEVEPTDHIIDNVNVFRDDEIGTSLNHDDIKHNAPQFDRGHFVVPKIIE"
     gene            complement(21313..21954)
                     /locus_tag="P5123_02165"
     CDS             complement(21313..21954)
                     /locus_tag="P5123_02165"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRX15097.1"
                     /translation="MASFFIFTLFSCASGVCLESEIVPESMIYSTEGEVGVTHSMFCD
                     ITVEYLDSKKWSRLNHGKPYGGVFASNQRIFPWYPFLVTVKNSGSQIISLGDFTLSFG
                     DYNVSALGKEDLKKLSRVLGVADLSALTNFRLYHDFQKSFDRMDIAGESTPYSFSRIL
                     PGDRLIFVVIFEAPPARFKRFSFKAQITGEKEKKIVELPLARRVYRVNDNRLN"
     gene            complement(21991..22383)
                     /gene="rpsI"
                     /locus_tag="P5123_02170"
     CDS             complement(21991..22383)
                     /gene="rpsI"
                     /locus_tag="P5123_02170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012743748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S9"
                     /protein_id="HRX15098.1"
                     /translation="MAQTKIYATGRRKESVARVYMSQGTGKITINRQPINEYFRRQTL
                     ELLIRQPLESTDSLANFDFEVFVKGGGWTGQAGAVRLGIARCLVQSDEKNKKSLRAAG
                     FLTRDARQVERKKYGHKKARKSFQFSKR"
     gene            complement(22399..22833)
                     /gene="rplM"
                     /locus_tag="P5123_02175"
     CDS             complement(22399..22833)
                     /gene="rplM"
                     /locus_tag="P5123_02175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014856352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L13"
                     /protein_id="HRX15099.1"
                     /translation="MIQKTYSMKASDIEKKWYVVDAQDKVLGRLATKVASIIRGKNKP
                     GFTPNLDMGDNVIIINAEKVKLTGQKSVQKEYFSHSQYPGGIKFTNIEKYMETNPAFV
                     IEHAVKGMLPKGRLGREMYKNLKVYAGSEHPHAAQKPENIEL"
     gene            23201..>23490
                     /locus_tag="P5123_02180"
     CDS             23201..>23490
                     /locus_tag="P5123_02180"
                     /inference="COORDINATES: protein motif:HMM:NF013468.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003725 - double-stranded RNA binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Sua5/YciO/YrdC/YwlC family protein"
                     /protein_id="HRX15100.1"
                     /translation="MIYHIHEETPNKRHISQAADILCSGGILIYPTDTVYSFGCDYSN
                     RESIAKINRLINRDPKKPLSIIINDISVMHNYVRHVSNEAFKIIKKTAPGPFT"
BASE COUNT         7802 a         4654 c         3988 g         7046 t
ORIGIN      
        1 atattcacgg aattcaacac tatctatctg gcccggctca acattatcaa ttcttttaag
       61 ctgaaattta acaagatgtc cattttcaac cgctgtttca gggtcagaaa gaacttctgt
      121 tgtagcagtg cgctctcttg aaatatcaat ttcattttga acatctaact ctgttatttc
      181 acatgctgct tcatcaactt taagtccctt atattcaccc agttcaactg taggcataag
      241 ttcgaaaaaa gctttatatt taaacggttc gtttctcagc actctgtcat aatgaatttt
      301 aggctccatt ataggcataa gttccttttc tttaacagcg tcaaaaaccg tactttttgc
      361 aagattttcc gctacctctt catcagcata ttccctgaat tttgtttcaa tcatctgaac
      421 aggagctttt ccctttcgaa aaccgtcaac cttggcattt tttcttattt tttcaaaaac
      481 agacttgtat tcaagttcaa ctttttctac aggtatttca atttcaagct caactatagc
      541 gttttcgtgc tttttttcac ttagaatatt caatgtacca tacccctttt aaaatattgt
      601 atcagcaatt tctatttata ttcatttata aaaatgtata aaaatgttta tgctaatttt
      661 aatatagatt ttcagcaatg tatatcaagt ttaaactata aattgacaat aagtatatga
      721 gtcaaacatt ttttccgatt atgtcctttt tatactctga tcagatcatg attcagcagg
      781 gagatcttag tgaaacagag aaaggaattt gaaaatttcc ggtattgcgg tcagtttctt
      841 ttcccgacaa ccggttgcta taaaaaaaaa agactgcctc aaaaagcagt cttttttaat
      901 gatgagcggg aaacgggatt cgaacccgcg acgtcaacct tggcaaggtt gcactctacc
      961 actgagttat tcccgcaact gcgagtgaag ggagtcgaac ccccacgccg aaagacacta
     1021 gatcctaagt ctagcgcgtc taccagttcc gccacactcg cgtcaataaa tcacatagat
     1081 atatctgccc ttatttttgt caaataaatt tcgaaacgat tagaattttt gtcccatttg
     1141 gttgaataaa aaattttttg gcacaaaacc tttcatatca ggggagctgt tcaaggcgat
     1201 acggagtaaa atcaatcaaa tcagctgaaa tagtgccatc ttcatccatc tcgatcagct
     1261 tgcactttac acgataatag aggccacggg atatatttcg gagtttacgt ttaagaacgg
     1321 gattttcctg ataatctttt atgctaaagc ggagattaat attttccata tcgtagaaag
     1381 atatttcgtt atagtttaag tcgcaatact gtaaacgcaa aacaaaggag acatccctgt
     1441 ctctgtaagt ttcgaagtct ttttttactg ttgtcaggcg aaaatccttt tcaaaatcct
     1501 ccgacacaat caaagtaggc aaacaaaaaa atagcaaaat aacaagacaa aacagccgca
     1561 aaaccaaata cttttttaag atatgaacga ttttcattaa tgactccgga aagtactagt
     1621 tactaatgaa tcggtccatt cttgtattgt ttaaactgtt tatcaaaatt atttcgattt
     1681 attgcactat gttgaattcg gtagaacaga tattcaacaa aaacccacac catcaagctg
     1741 gaccaaaaaa ttataactgc ctgcataatt gtgattttca taacggactc ctttgtactg
     1801 tcttatataa agcattttgc gtgccacatt tacgattttc ttaacaattt gtttgtttat
     1861 ttccatatta ttaaggattt ttctcaacat ttttctctta ttgtttacga ataattcgca
     1921 aaaatcagag aaaaagccta tttggtctat acgtatcatt aatacaacgt acattatata
     1981 aaacaacaca gggcaattat cagacacaat aaaaccaatc agtaaaaatt tgtgaaaaat
     2041 aattgtactt ttatcagcaa caaaagatta tgatttaatt ggtatttttt aaaactaatt
     2101 cgatgttcgg agtctatctt gaaaaaaatc cttattttca ccctctcttt tatcttggtt
     2161 agcaccattt ccgagtatgc ctttggagaa acagcccgca gatatatgat aatcacaaac
     2221 agtgtcttct gcaaaactgc accctcttcc gactcgacag acatggaagc cgacattggg
     2281 tttggatctg ttatcctggg agaaaaagtt attcaaaacg aaacagattt cgattatata
     2341 aagctttccg gaaaagaaga ttgttacgtt ccgttagaaa tgacaataga agttccgcta
     2401 gtttcttttt cctctgagaa aggagccctt agcagtgaaa acggaaaaga tcttcccatt
     2461 ttttttgtac ctaatgatct tatgccgatt aacaagatgt acagttttgg agataatcgg
     2521 gttcttgcca aaaaatcgat attgaaagat ctttacgcga tgctctctga cgcaaaaaaa
     2581 aacggacata aattttatgt ggcaaacggc tttaaggatc ttcaggttag aataaaggag
     2641 tacgaagaag cgatcaggtt aaaccctaaa cagaccttgg taccaaaacc ggttcactcc
     2701 tcatcggcga ctggtctggt tatcgacctg acagataatg taacaggttc caattttgag
     2761 tacgaattga aaaatgatca agcatggaaa tggatatatg aaaactcttc acgttacgga
     2821 ttcagaacaa gcggcaaatc tttccgattt gaatacagcg gtgaaaaaag tcgacgaaaa
     2881 cccaaaacag ataagatttt tgatgaagcg attaacaagc gcattgaaat tccctcttta
     2941 tgtactgttc atgtactaaa aaactctacg aacaaaaggg caagtttcat ctgggttcac
     3001 cacaacgaaa aatcggccgg taaaaccctt aaatatgcac tttcaaaata tggtggccac
     3061 agcatttata ttataaacgg agacaaacgc aatgtctata tcccatacaa ccgacgaagt
     3121 tactttatag acccaaaccg gatttacacc cccaagggaa tcgagaaata ccttgataga
     3181 catcatcgaa aactgaataa aaatgggcgc cgctccgttt ataaaaaagc agagaagtta
     3241 aaagatacga ttctctcttc aattgactgg accgaaaaaa aatatctaat agcccttcat
     3301 tcaagccgtc cgggatcaga tttttcaatt gaagagttta atgctaaaaa ttcttatctt
     3361 acttacacta atcccgacca caaccctaaa aacctgtttt tcaccacatc tctacaagat
     3421 tattattttt tcaaaagtag aggatataac gtagtttacc aaaggtccat cgaagatgat
     3481 ggttctttaa gtatatatgc ggcaaaaaac aacctaccgt atataaatat agaggtcgta
     3541 gagggagatg acaagttcca gcaaaaaatg cttgatctgg taatcgaatg tgttaaccaa
     3601 cactttaagg aaaaatccct ttaatgcaaa tcgtgaatac tttttagaaa agtatccgtg
     3661 aaaatatact ccgggaggaa caaatgagaa gtgatcaatt taaaacaggt attggtaaag
     3721 ctccgcaccg ctcattactt aaagccagcg gactttcaga cgaagagata aggcgcccca
     3781 ttattggtat tgctaactcg gcaaacgaga ttgtgccggg tcatatacat cttgatacta
     3841 tagtcgaagc agtaaaaaag ggaatattgg cggctggcgg tacccctctt gaatttacaa
     3901 ctatcggggt ctgcgacggc cttgctatga atcatatcgg aatgaaatat tctctgggtt
     3961 cacgtgaatt gattgccgac tctatcgaaa taatggcaat ggctcaaccc tttgacgggc
     4021 ttgtaattgt accaaattgt gataaaattg tgccaggtat gattattgct gctgcccgat
     4081 taaacatacc tacaatcatg attagcggag gccccatgct tcctggaaaa tatgttgggg
     4141 aaagtgttgg ccttgataat gttttcgaag ctgttggcaa agcggcaaaa ggcgatatga
     4201 ccgaagagca gctctattca cttgagtgct cagcttgtcc tggtgttggt tcttgcgcag
     4261 ggctatacac cgccaattct atgaactgcc taagcgaaat tctgggtatc tctttgccat
     4321 ataacggttc aataccagca atacatgcgg ataggatccg tcttgcaaaa aaagctggtg
     4381 cacaaatcat ggaactagtg gataaacaaa ttaagccacg tgatcttatc actaaagatt
     4441 caatttacaa cgcattggca gttgatatgg ctattggtgg ctcatcaaat actgctttgc
     4501 acatacccgc catatctcac tatgctgagt gtccgatcac actaaaagat attaaccagg
     4561 ttgccgaaaa agttccgcat ctttgctcac tggctccagg gggaacagat tatatagttg
     4621 atctgcaccg agctggcggt attccatctg ttgtaaatga actcatgaag cacggactta
     4681 ttagggaaaa ctgtatgact gtaacaggaa aaaccattgg ggaaaactgt aaaaacagtc
     4741 caataaaaga cacatcagtg attagaactt tcgaaaaacc tgtacataaa gacggagggc
     4801 ttgctgtatt aacaggaaat atcgccccgg atggagcaat cgtaaagaaa ggtgctgttg
     4861 atccttcaat gctgaaacat tcgggacctg ccaaggtttt tcattctgaa gaagatgctg
     4921 ttgatgcaat aatgaccggc aaaatagtaa aaggtgatgt tgttgtaatt ctttatgaag
     4981 gccctaaagg cggaccagga atgcgcgaga tgttaacccc cacagcaacc attgcaggta
     5041 tgggtctgga taaagaagtc gctcttatta cagacggccg tttttctggc gcaacgaagg
     5101 gcgcctctat cggccatata tctccggaag ctgcatcccg aggaccaatt gccgccgtaa
     5161 gaactggaga tattatcgag attgacattg ataacaactc gctgaatgta aaattatctg
     5221 atgaagagat acaaaaaaga attgcggagc ttccggagta tgtctcaccg atcaaacggg
     5281 gatatatgaa tcgttactca aaacttgtct cttcggccga taagggagca gtttttgaat
     5341 tatagaaaat taccaaaatt aaactttgga taattactat aattatttaa gtaattccgt
     5401 ttatatgcct actttaaatt gattttcgcc agttcattgt tggcagataa aataataaag
     5461 attaccaaaa ataatagccg tttgtccaaa tttaaacggc tattttttta tttcacacct
     5521 gcaagtgaac tcttagatat ctcctcctca gaatcaggcg tgcatatatt ttacaaatta
     5581 tggttcaaga caagggttta atatcacaat taaaggggaa aatcgccatc ctgacagatt
     5641 ttgatttacg gattaccctt ttaaaaatca gtgatttgtt tctctttcct ttttttagat
     5701 aatccttaag agaatagagc ctacgcggaa gctctctaag tgccgcctga actactgcgc
     5761 taatatcttc ccgtcttacc gcatagtatt tagcgtaaaa ttcagggaag ataagagtat
     5821 tgataggcat caagagcaga tgaaatctgt tattattgct gtattcatca aagtcagtcc
     5881 acaaacacat cccctcccaa aaacccgagg caaaagttaa acctttctcg caataatcac
     5941 tccacatctc aatatgtaat gagaattttt taccctctat tgcaagtgaa agctctttag
     6001 cagttttaac caaaaaattc ttaaacccag caacattatc cctgtttttt ttatcccaac
     6061 tgccaagacc tcgcttgatt agatatttta tccatatttt ttgctcaatc atgtaaggat
     6121 tagttgcaac agccgataaa aaaccataat agaacaaaaa atccgctggt tgatgaaaat
     6181 tagattctag ctcatctttg atacttttaa gaatattttt ttccattact acctccgttt
     6241 gaccaccagg tctttatata taaactggat ttttctgatt ttgttaaatt ttttttaaaa
     6301 aaattgctta atgtaaggtt actattatca ctctttttat caggaacttc gaaaaaacgc
     6361 tcaaaattat cgcttgttat tgattatttt tttcgcgagc tcaaaaaagc gctaaaattt
     6421 aagctttaaa gacagcttca aatttcagct tagtaagcga tatttatggc tttataaaat
     6481 gaatttttcg caagttcctt gttaaaagtt aatttttaga tgttgcctat cactatttaa
     6541 actaaaaatg ttgactatca tcgaacttga tattttcttt atcttaatct cataataact
     6601 tggcaaagaa agaattgtat atataaaaag ttagaatcat tcttctctca agagaacttt
     6661 gccaaagagt acttaagaaa tttaaccgga aatgattagg tgtatgaata aaacacaaaa
     6721 tataaagaag gtacttttac ttgatgatca gcagatgatc ctgacgaccc tttcaagaat
     6781 gattgaaatt ttaggcggta ctgttaccac ttctactgat ggcaaagata ctctagaact
     6841 atacaaaaac tcatttgaaa aaaatgatcc ttttgatcta cttatacttg atatttcaat
     6901 agacaacggt attggtgcag atataattat tgatgatctg ttaaaaattg atccggatgt
     6961 aaaagccatt gtttcaagcg gagatcctaa ccatgatgca gttataaact acaaagaaag
     7021 aggttttgct agtgtacttc ttaaaccctt cacaatagac aaactaaagg atattcttga
     7081 cggtttagat taaatagatt attcacacaa tacttgttta ttttacttcg agacttttaa
     7141 tatcatcttc ttcaagatgt tctttatact gaatcatata cagcctgtaa taacgacctt
     7201 tctttgacat taaatcgtta tgcgatccgg actctgttat tgatccattt gaaataacca
     7261 ctattttatc actgtgacgt attgtggaaa gtctgtgggc aataactatg gaactttttt
     7321 cttttagaat acggtttaat gccttttgaa ttgcatgttc agtttcggta tcaatattag
     7381 atgtcgcttc atccagaatg aaaatagcgg ggtcatatgc cactatgcgc gcaaaagata
     7441 tcagctgtcg ttcaccaaaa gagagagatt tgccttcttc cgaaagtatg gtgtcggctt
     7501 tatacggcaa tttttcaata aatgatgtgc aacttgcgta attacagaca tcttcaaaag
     7561 accttccgcc tgcaggttcc cgacctagaa gtatattgta acgtaccgta tccgaaaaca
     7621 gagtaacatt ttgctgaaca acaccgataa gtcggcgcag agatttttta ctgatactgt
     7681 ttatgttaat tccattaata tatatagcac ccttttgaat catatacatt cggctaagca
     7741 acttgattat cgtactcttt ccgcatccag tctctcctac tattgcaaca acttccccat
     7801 gagaaatttt catggaaaaa tctctaagaa cccagtcttc ctccctatat gcaaaccata
     7861 cattacaaaa ttctataaaa tattctgatt tttttgcaac gagttctcga caactgccac
     7921 tttgactaca atcatactca ggctcttgat ccataagttt aaaaacccga tcagcagatg
     7981 caagagcgga ttgaactatt tgaattttct cggctatgtc ctgtataggt ctgaaaaatt
     8041 tctcaagata ggctataaag actacaagag tacccaaggt tataaacccc cgagctacat
     8101 catttgatcc tagatatatt atatacccca taccgcaata tcgcagcata ctgatcatcg
     8161 gagtaaatac agaatttata aaaacctgac gagatgacgc attacgataa tttttgctct
     8221 ccttgttaaa tacattaaga aaggcactct gctgtttgaa tatctgaata accgcaattc
     8281 cagaaagtgt ttcggacaat cttccattga caaccgcaag agcctccctg acacgagtaa
     8341 atgatcgacg catcttttta cgaaaaattg ccgatatgaa agccgcgaca ggaagcattg
     8401 ccatagtcac aagtcccaaa cgcaaattta taacaattaa tacagcaaca ataccggtta
     8461 ttatcgcgat atccttgatt atattaacaa ataccgcggc aaagaactcc tgtatgtttt
     8521 cgatatcatt acttattcgg gtaacaattt tccctgtttt atgttcttca aaataacgaa
     8581 ctggaagatt aacaactttt ttatacaaag ccatgcgtat atcatggata acatgattgc
     8641 ttgaatagct tacaagtaac aactctgcat aagctgtaat aaaaccgaca acagttaacc
     8701 caatgataat catgaatatt ataacaacac tttctatgtc atgggcacgc aattgacgaa
     8761 tagcatccct gtttaacgac acaagattgc ggtaaggaac aagataatat ccctcctttt
     8821 ttatcattgt ttcgagtaat ggtccactat acttatcact cgaaaccaga taaaaaagct
     8881 caaatagatc tttattttct gcgattattt gacggttctt cttgctgagt tcactaattg
     8941 aaccgtaatc gactagaagt gacccatcac tgaacatttt gatttcattt gatagattat
     9001 ttgtttcaaa aagagctgca tctttttttg ttatgtaata ataacgcctg ccgatatgct
     9061 tgtctattgc ctgccttgtt aataccggta caactaactc ggcacatacc gcaactatac
     9121 ttaaaaatag agcgccgata aagaaaaagc ggtatggtct tacaaaaaag gcaacttttt
     9181 tatataattg aatatttgtc ataatctgtt ctgtaccagc tgccttgtat aaatatcaga
     9241 atatagccct ttttgcttta caagctcttc atgtgtaccc ttctcggcaa ttgttccctc
     9301 atcaagaaca taaattatat cgcaattcga aatggctgat accctgtgtg aaataatcac
     9361 cattgaaccc ggtattttga gaagagaatt atataccgat ttttctgttt cgctatcaag
     9421 cgcagagagt gaatcatcaa taagacagag aggtgccgga gacaaaagaa gtcttgcaag
     9481 agctaccctc tgtttctgtc cacccgaaag attgaccccc ttttcaccaa gcatcgtgtc
     9541 cagttttttt ggaagatcat ttacagtatc actcaattta gcatcactac aagcgattct
     9601 taactgctct tctttatcat tactcgatcc gaaaactatg ttctgtctaa gtgtatccgc
     9661 aaacagagtg ttttgttgcg gtgatgactt gatatgagac aatatatact ttactggaag
     9721 atctcgataa ttttgtccgt ttaacagcac agaaccagtt gaagggtcat aacgcctaag
     9781 caaaagatcc gcaagggtgc tcttacctga accggttttg cctgtaatac cgattctaca
     9841 gccataaggt attatcatag aaatattttt taacgcggga tgaagcgcct ctggataagt
     9901 aaagcttaca tctttaagtt caaacgttgg ctttggtaag tactgaggct cggctatcaa
     9961 tggtttatct ctaacaacag cattagcatc cagcgcgtcg ttaattcttt tcattgaggc
    10021 gcgccctctt tgaaaaagat tcattaccca gcctattgcc atcataggcc atgcaagaat
    10081 ggatatatag ctaaaaaaag ctgtaaactc tcccagtgaa agttccccca taatagtcat
    10141 ggttccaccg cgaatacctg caaaagcagc cgctatgcca gttacaagag ccattaaagg
    10201 gaataaggcc ccccacacac gcataagaga aaaattctga tcacaaattt ttacagaaga
    10261 cttttcgact gaacggatac tctcattttc gagtgtaaaa ttttttataa gtacaatact
    10321 agaaacatat tcttgcaaac gccctgtcaa atcagaaaaa tattcctgaa cgcgagtaaa
    10381 accaccgaat atttttttcc ctgcgattat caaaaaaaag gtaattactg gcaagggtat
    10441 aagaacatac atggtaaggc gggaattcat tgaaaacata aaaactactg atagtatcgt
    10501 aagaaaaatt ccatcacagg ccgaaaccac tcccatactg aacatcatac gaattgcctg
    10561 aaggtcattt gtagcaagag ctataagaga gcctgttgaa cgtctgtcaa aaaacggctg
    10621 atccatatat aaatattttt tataaagatc atttcgaagt tgctcttcga tataaaatga
    10681 tgttctgacc agaagcatac gccaaaaaaa acgaaaaatt gccattccca gagcaagaag
    10741 aagaatatgt gaaaaccaga tatgtatacg actagtatct agagcaaaaa gatcatcgat
    10801 catttttttc acggcacgag gaataaagag ctgaaagata tcaaccagca gaagagaaat
    10861 tatacccgct gcgaaatagt agcgcctttt aagaagataa tagagaggac gtcgaaatag
    10921 tgtcatatcc tattattaaa cagcttataa aattgtaaac caatttagca taaaactaat
    10981 taagccatat tgaagtatta acttcgatat ggcttaaata attaattaca atcaccaatg
    11041 attactgcta atctaattat tttttatcga ttcgctcaat tcgtaacgca atatggcatc
    11101 cataaatttg tcctttggat accccttcat aagccttcca ttgatgatca aggtcggtgt
    11161 agcactgatc ttcagctttt tggcaagatc aagatgtttt atgattcttt cagagggctt
    11221 gtcagaatta aacatctcaa ttaaagcttt tttctgttca tcactctctg caacaatttc
    11281 aataatctta tctatatgcg caccggaaga ggaaagttcg gatacttcat caaatttttc
    11341 ataaaatttt tcaaaaattt tgggataaat atttaacata tccgcagcat aaaacagacg
    11401 tgttactgcg caaataccat ttataactat tccggatttt gccggatcac aatcgctgtt
    11461 taaagggtaa tagtattgtt taaaatttat ctttccttta tactttttta ctaaaacttt
    11521 ttcgatcatg ttaaaattac gacaagctgg acaaaaaggg tctgtaaaaa catgaacagt
    11581 taaaacagca tcttttttgc caacaacaaa cccagtttcc ggataatcaa tttcaagaat
    11641 ctctttttct ttatatttct cgacatagcc tgtcagtttt ttgttaagtt ctgatccttc
    11701 actgcttcgc tcttcgagat aaagagaaaa agcaacaaca aatataaaca gtatcgcagt
    11761 aaaatatacg aaggtacctg tggaaaattt acgatcatca ttttttccag aaatcttagc
    11821 aaatccaaaa agtgcttcct tgaagctcat atcacccgat gcacgcattt tgcgatatgc
    11881 caagattact acggcaagaa tagctatatt tacgacatat gtcatgacac agtattgaca
    11941 aaatacctga agatagacaa gaagcgctcc aagaacaata tcgaaaaaga tcccagcaac
    12001 actaatgggt atcataaggt acatgaatgt tttccagtag cgctcaccag actcgcgaaa
    12061 cagatgcaaa gaaagaaata tccataagta gaagaaaaaa ccgtaagatg cgatcggaat
    12121 tccaaaaaac atagaatagg gcgatgaaga aagttcggaa caggagcctt gtaatccgtc
    12181 accacaggct aaaaactcag cagttttttg aggaatatag tgatcagcaa gaagaataac
    12241 agccaaaatc aatccaacaa atgagaagat cgataaaaga atatatgttt tgttcatttt
    12301 taccacctag caagattcca aatatgaaat tattagctat tatttattta cagtgtgtca
    12361 acacataaat ttcatcagat aactcaaaaa ccataacgat acaataacat ttttttgatt
    12421 attattacgc aaacgcgtgc atacactaga aacttcgaaa cattatcata cttcagaggg
    12481 taatatcgtt taattgatat taatatcaat tttaagttta ttagttgtca ttggaaaggc
    12541 attacactat ttatgagtga tgattccata attacaaata tataaatgtt atacaaaggg
    12601 taactatttt gaaattatct ttttttaaga ctttcaaaga gctggcaata aacacatcca
    12661 ttttagccat attttttgtt tttatttgca ccataaccct tatattatat ttgagagtca
    12721 gcactcttgg cgaaaatgat gatcccgggt tcatcaccaa gaaccccaac gaataccggg
    12781 aaattgatgc tattccgata ttgctttacc atgatataga cggcaatggt ccttactcta
    12841 tcacaaaaga acagctcaga aaacattttg aattttttcg attaaataat atcgatgtta
    12901 tatctttaaa agaacttatt aggctacaac atgaaaatcg cagccctgaa aacaaatcgg
    12961 tcgttataac agtagacgat ggttatattt ccgcatatac caaaatactt cccctgatgc
    13021 aggaatttga ttatcctgtt acgcttttta tttacactaa tttcattttt gaaaaatcag
    13081 ataaacacat gacctggaca atgctgaaag agatggaaaa gtacggattt gacattcagt
    13141 cgcataccgt atcacacgct gatttggtta aacttatcgg aaaaggggac actgaatctc
    13201 tcaattcgat cgaatacgag ctaacacaat caaagaaaat tattgaagac aagcttgaca
    13261 aagaagtaac cttactcgca tttccatacg gatattacac tcctaaactt ttggatatgg
    13321 cgcgaaactc cggctatatt aaaacatttt ccacagatta cggtgcaaat atttcattac
    13381 acagtaacta ctgttatagg cgtcaccata taaaaaaaga tttcaacgaa actaatttaa
    13441 aaaaaataat tcaaatgaga tgataaaaaa aataaccatc ataagttgaa ataaaagatt
    13501 atcttgactt tactgaccta aaaaatatct gtatccacat gattaaatat gatttacaca
    13561 cgcactcgca attttctcca gatgctgaag atactgttga cctgttatgc cttacggcta
    13621 ttaagaaggg aatcacacac ctcgcgatta ccgatcattt tgacgctcat aaagatgata
    13681 cttatccttt ttattacaat tacgaaaaac agagtatcgc gattgataac gcacgtgtta
    13741 agtacaaaga taaacttacc ctgttgaaag gcattgaata cagccaaccc tataacacac
    13801 cagaaatttt tgcccagacc ataaaacttg agtttgatat tataatcggc tcaattcata
    13861 agataggtca ttataacgat tacgataaag acaatgatac aaaatataag aagtatttca
    13921 caaatattaa gcggatgatt gaacatggcg gcttcgatgt attaggccat atcgattttc
    13981 cagctcgcta ttattcaggt tactactcac acagcaggga attggaagat gctgtaaaag
    14041 cactatgtaa aagcgaagta ataccggaga taaatagctc tcctttacgc agaggcatgc
    14101 cttttgcaat gccggacgaa ttgatattgc agatgtatac cgactttgga ggacgttata
    14161 tcactaccgc gtcagacgct cacaatgcca atgatattgg tgccgatttt gattacattc
    14221 aaaaacttat caaaaggttt aatctgctcc ccgtctattt taaagatcgt aagatgtttg
    14281 aataatatct cacaacacca ctcgtctgaa acataaaaca agcaaaatat tgacaaaaag
    14341 gttttttaat aatatcttta gtttaaataa gccatgagtt cggctaataa cgaatctgta
    14401 cccagattat ctgggttaca aagcctataa aatggataaa ttatgataac cgaaaaaaaa
    14461 atactaatag ctgaagatga cgaatcttca gccgaactgc tccgagcagt atgcgaagaa
    14521 catggttaca ctaccgacat agcttctaac ggtaaagatg catacaaaat gtacagacaa
    14581 gacaattacc cacttgtaat aaccgacctt gagatgcctg atatgtcggg acatgatcta
    14641 attaaaaaca ttaaaaaaat aaatgatcgt caggtaataa ttgtctctac catgcatgcc
    14701 gactcggaaa caataatttc tgttatgcga gaaggtgttt acgactacat cattaaacct
    14761 attaatttga caattatcat ccagcagatt aaacgagcat ttgaggctga tgagctttat
    14821 cgctcaagag aactaattga gcaagaacgg ataatcaaac tggaaaatca gctcgagtgg
    14881 gtacaatgga acaaggagat tattaaccga gacaatgaca gaattgacac agcacttttt
    14941 gacagtctta acaatagttt taatcaggga gccggctttg gagctcttct aagccttatt
    15001 aatcttgttg cttcttcaat aaaaaaagag ggggatcact atgtagtgcc ttccgatatt
    15061 tttgatctta tcaaatcaaa cgccgccatt gcagaaaaaa ctttgaactt cttttctgac
    15121 ctatcactat taatgaaaaa cgatataagc ataagcaact ccacatgcat ggatatttac
    15181 gaccttcttt tagagattgt taattccgat aatattaaaa acttatccaa tttaagaggc
    15241 catacaattc ttctgagtaa tattccggct gatattaaag acacagtctg caaaatcaat
    15301 ccaagataca tatctaccgc aataaacgag ctttttgtaa acgctttaaa attttcgact
    15361 tctaattcta aaatcaccgt aatgcttcgc tactcgggtg gaaaatttct aatatcagta
    15421 ctcaatacgc ctcaacctac taaatcagat attataggaa taccaccaga ataccagaat
    15481 gtggtttttg aacctttttt cagacttgtt aaggtagtat atgaagatta tggaactttg
    15541 gattacggtc tgggtctaac attggtagac aaaattgtcc ggagccatgg aggccgtgta
    15601 gacataggaa atgttattga tcatacagat ccatcacgcg atccagtgac taaggttctc
    15661 gctcagattg ttttgtaaac aaggggattg aattttagac aataaaaaag aacccttgcg
    15721 ggttcttttc ttatctaaag aaaacttgtt taatcagttt ttatctttat caacaatttt
    15781 gcctttttcg atccaaggca tcaaagctcg aagcttagca ccaacctttt caattgggtg
    15841 ctccatccag atagctcgct tggctttaaa agaagcctgc tttactttat tttcaagcat
    15901 ccagtcacgg gcaaattccc cctcctgaat acgacgcaaa acttctttca tctttggctt
    15961 gaccgtctca tcaataacga atgggcctgt tacataatca ccatattctg cagtatttga
    16021 aattgaataa cgcatatttg taaatccacc ctcgtaaata aggtctacaa taagcttcat
    16081 ctcatggata cactcaaaat aagccatctc ttccggatat ccagcctcta caagagtttc
    16141 gaaacctgcc ctcataaggc ttgttactcc accacaaagt acagcctgct cgccaaaaag
    16201 atctgtttcg gtctcgtctt taaaagtagt ctcaataact ccagcacgag caccaccgat
    16261 accattagca taagcaagtg ccaaatcaag agccttaccg ctggcatttt tttcgatcgc
    16321 tactagacat ggtacccctg cccctttttg atactcggca cgaaccatgt gtcccggacc
    16381 tttaggagca accattacaa catcaacatc agccggagga ttaatctgac cgtatattat
    16441 attaaatcca tgagcaaaca taagcatttt acccgaagtc aggtttggtt tgatgtcaga
    16501 gtcataaacc tccccctgaa tttcatcagg tacaagtatc atgatcatat cggcccattt
    16561 aactgcttca cctacttcac gcggagttat acccgctgct tcgacctttt tccatgacgg
    16621 actaccttta cgtaaaccga ctgtaacttc aacgccggaa tctttaaggt tatttgaatg
    16681 accgtagcct tggctaccat aaccgataat agctactttt ttacccttta aaagaccaag
    16741 attggtatca gattctctat ataccttcat aaatccccct gattgcattt ttttactact
    16801 atccctgaaa aggtgaaacc gtcaataaaa taatccagct gcttgaaaaa agttaagtat
    16861 ttattaacaa actttaatat tacttaagtc tctttatcga aagatagata attttaaatt
    16921 gtaaaaattt attatagatt atttttaata ttagcttgac taagacagtt tatgggattt
    16981 tatgataaat atcagtacaa tatagagcaa agacaaacat aatggtatca tatggatcgt
    17041 gaacttcgca aacatttttt aaacttgctc atatactctt ccctaaatac tcaacaactg
    17101 aattatttgg gcgagcaaat agacaacagc tttaaccttt atcgggaatc cggcttcgga
    17161 gatacaatcc ctatcccccg tcaatcggca gcaaaaatcc ttgttaacta tttcactgcc
    17221 gaagaggata ttgtgaactt gtttacaacc cttctagcaa atcaaggtaa acggttttac
    17281 aactcagaac tacgactaaa cggatatgaa gattttataa agtttttggc aaaaaaaaag
    17341 tggatatttg ataaccaggt ttttcagttt tttagagatc cttttttcga gaatgaaata
    17401 aacgtattaa aatcaatacg cataatcgat ttacgaaaaa aagacgaaat agattctttg
    17461 ctaaaaaaaa tagaggacgt tacccaaacc ctaggcgaca aggatcttga gtggagcgta
    17521 aatcttcgac tttatgacct tgacagggag attagccaac tcattcgcaa aataatcgga
    17581 atgcttctta ttaggcaaaa tcttcagtct ataacctttg atatttttac atgtcttaaa
    17641 gaactggcta taaacgcaag caaggcaaac tataagcagt tgtttgacaa gcttgttaca
    17701 aaacctgaag gcatagatgc caataaaaac tactcactat ttttaaaaag attcaaggaa
    17761 gaaattgctg aaaacggaaa tcgcaaccta atcaaacttg ctcaacaaaa agaccgctat
    17821 ataaatatca ctttccaatc cactaaccag ggtattggag tttgggtaac aaacagccaa
    17881 aacataactg cggtagagaa aaaagctatt ctcaagaaac ttggctacaa aaagacagat
    17941 atctttgcct atgccgacga taaaaatagt gaaggtgctg gactaggcat tggcttggta
    18001 ttgtctctgc ttcggatcta ttctgatgag gatatccctc taaaagttgt cttttaccct
    18061 aacttcataa agattggttt ttttctggac agatccctga ccgcggctcg actacaggag
    18121 aaactcgaaa ctcaaaagga acagaattaa aaaagaccct ttgcacaata aaaaccgtcc
    18181 ggacgttatg cactttttat ttttttatgt ttacaaattc actattctta gtttaaagat
    18241 atttatatta attattttaa aaagcagttt aactttttta tttttgtctt ttcccttctt
    18301 tactatcttg caatcgacaa aatttatagt caaaactgtt atgtaaaaca tattttgtta
    18361 cccattttgc tcctcctaat acaaaagcaa tatagactta acatagttcc actgcattca
    18421 gatcatggtc accctaaagg tgaccatgtc tttatcaact ttcttcgtaa aaatcacttg
    18481 aaaatcagcc taccgattct taaatgatca catcttttgt tttggactat agcgaaagac
    18541 gatttgcaac cttgcttttt taaggatata catatatgaa aacctacgaa gagataaacc
    18601 gaaaaatcgc atctaaaacc gcggtggtca tgaacgcgga agagataatt gattatgtag
    18661 aaaaaaaggg tctcgataaa gctgccgaag aagttgatgt tgttacaaca gcgacctttg
    18721 gacctatgtg ctcatcagga tgttttctga actttggcca ctcgtctcca aaaattcgaa
    18781 ttacagaagc ctggctagac gatgtgcttg tgtattccgg aattgctgcc gtggatgcat
    18841 ttcttggagc gactcagctt aggcataatg accctgcaaa tatgtattac cctggagact
    18901 tccgcttcgg cggggctcat gtaatagaga agctggttcg cggagaaaag gttcagctgt
    18961 ttgcattatc ttacggaacc gacgagtacc catgcaagga actgcgcaca tacataacta
    19021 tagatgatct gaaccaggcg cagatggtca atccacgaaa ctgctatcaa aactacaaca
    19081 tagccgtcaa ctgttcggac agatctatat atacctattt tggccatctt aaaccaaata
    19141 tgcagaattt atgctactgc tcagctggtc aattatctcc tcttcttaat gatccttatt
    19201 ataaaactat tggtattggc actcgcatat ggcttgccgg ttcccacggc cacatctatg
    19261 ccgaggggac acaacattca cccgactgtt tacgtagcga aaacggtgtg ccacgagagg
    19321 gagcgggcac cctgtcactt acagctgaca tgaaaaaaat ggatccggaa tatgttagag
    19381 cagttagtct aaaaggatac ggcgtttctc tttccttagg tgtcggagtt ccaatcccta
    19441 tactcaacaa ggaaatacta aaaagcacaa ctgtccgcga cagggatatt tatgctccat
    19501 tgattgacta ttccagcgac taccccctcg cgacaggaag aatacttacc gagtataact
    19561 atgaatcact aaaaagcggc gaagtagagt ataacgggaa aaaaattgaa gtcggctcca
    19621 tctcatcata cagcaaagcc cttaaaatag ccaatcgatt aaaagaagag atcagtgttg
    19681 gaagttttac actcgctgaa ccacacaccc cacttccggt aacaggatca atgaatcgaa
    19741 tgaagataca acaggagcgc taaatgatta caagaaaata tcttcttaaa tttcctgaga
    19801 aagcagttgg caaacctttg atatatcatc tggtcaaaga ttacgatctt acagttaata
    19861 tttaccgggc aaaaattgac cccgatgata ttggctatat ggttttggac gtctctggtc
    19921 gggaatcaga tattgagcgt gccaagattt tctttgatga aaacgaaata atcgtaaccg
    19981 aaaccatgaa tggtatgaga tgggataaag agaaatgtgt aagttgtgga acatgtatta
    20041 cacattgccc atctaatgcc ctctatattg ttgaccgaaa aactatggaa gtctctttta
    20101 acaatgaaaa atgtatagaa tgcatgaact gtataagcca ctgccctttt aaggcatgca
    20161 gctcaatttt tagtagttcc atatcagaat gactccagaa gagaataaat tcacatataa
    20221 agactccaat tttacaatac attgctcctt tttctctgag gcaaaagatg agataatttc
    20281 tcaacgcaca atacttgaaa aatacctaga aaaaaacccc aattttaaat catcattaac
    20341 gccattaaca cctaccagag atgcgccatt aattgttaaa accatgtgtg ccgcggctga
    20401 aatatgtaat accggcccca tggctgcagt tgcaggaaca atagcacagt ttgcctggca
    20461 aaaagtcttc gatatcgcca ctaatgataa aataatcatc gaaaacggtg gagacattta
    20521 cctgtcttcc aattcaccta tatatatcgg tatttttgca ggtaaaagca ttttatcggg
    20581 gaagctcgct ctcaagatta ctcctgaaga gactccatta ggaatatgtt cgtcatccgg
    20641 aaccatgggg catagtctta gtatgggaaa ctgtgatcta gcaactgttt tcgcaaaaaa
    20701 taacgctatc gctgactgtg cagccactat ggccgcaaat attgtaaaga cagtagatga
    20761 tttggaaaaa gcagctgatt accttaattc aataaacgag gttcgaggtg gtcttattat
    20821 taaagacgac cgaatagtca ttgttgggga aactcctgaa ataattaaaa acagcgacaa
    20881 aaaattaaca actaaaataa caaaagccga aggatcaatt ttttcatttt gaaataatga
    20941 ataactatta cgattaacct tttaggtaag tctttagtta aagctcttct ttaagttgct
    21001 tattcagttt gttattctat tatttttggc actacaaaat gtcctcgatc aaattgagga
    21061 gcgttatgct ttatatcatc atgattcaat gaggttccaa tttcatcatc acgaaaaaca
    21121 ttaacattat caataatatg atccgtaggc tcgacctcgg aagtgtctag agtattaatt
    21181 ttctcaacat actcaagaat atcggatatc tgtgaaacaa actgcttttt ttcatcttca
    21241 tcaaggtgaa gtcttgaaag ctccgccgta cgctcaacaa tacttatatc aattgacatc
    21301 tgttctctcc atttagttta accggttatc atttactcta tatactcgcc gcgcgagtgg
    21361 cagttcaact atttttttct ctttttcacc agttatttgt gccttaaagc taaaacgttt
    21421 aaatcgagcg ggaggtgcct cgaaaattac cacaaaaatc aagcggtctc ctggtaaaat
    21481 tcgtgaaaaa ctgtatggag tagattctcc ggcaatatcc atgcgatcaa aacttttttg
    21541 aaaatcatga taaagccgga aattcgtcaa agcagaaaga tcagcaacac ctaaaacacg
    21601 ggaaagtttt tttaaatctt ctttaccgag tgcggaaaca ttgtaatcac caaaactcaa
    21661 tgtaaaatca ccaagagaaa taatttgcga cccgctattt tttaccgtaa ccagaaacgg
    21721 ataccacggg aaaattctct gattagaagc aaaaacacca ccgtaaggtt taccatgatt
    21781 taatcgactc cattttttag aatcaaggta ctcaacggtt atatcgcaaa acatcgaatg
    21841 cgttacaccc acctctcctt ccgtggaata tatcatgctt tcgggtacga tttccgattc
    21901 cagacagacc ccacttgcgc acgaaaatag cgtaaatata aaaaaagatg ccataaggca
    21961 tcttttgaaa tatctgtaaa aatgcattga ttaacgcttt gaaaattgaa aactcttacg
    22021 ggctttctta tgtccgtatt ttttacgctc aacctgacgc gcgtcccgag taagaaaccc
    22081 tgcggcacga agtgattttt tattcttttc atcagactga accagacatc gagcaatacc
    22141 cagacgaaca gcaccggctt gtcctgtcca accaccccct ttaacaaata cttcgaaatc
    22201 gaagttcgca agagagtcag tagactcaag aggctgtcta attagtagtt caagagtctg
    22261 acgacgaaaa tattcgttta ttggctgacg atttattgta atcttgccgg taccctgact
    22321 catgtaaaca cgggctacag actcttttct tctgccggtt gcgtatattt tggtttgtgc
    22381 catctatgat acctctgatt ataattcaat attttcaggc ttttgagcag catgtggatg
    22441 ctcactgccc gcgtaaacct taagattttt atacatctca cggccgagac gaccttttgg
    22501 aagcataccc tttactgcat gctctattac aaatgcagga ttagtttcca tatatttttc
    22561 aatatttgta aactttatac cacccggata ctgagaatgg ctaaaatatt ctttctgcac
    22621 acttttttga ccagtaagtt taactttttc cgcattaata attattacgt tgtcacccat
    22681 gtcaagattg ggagtaaatc caggcttgtt ttttcccctt attattgagg caaccttggt
    22741 tgcaagccgt cccagaacct tgtcctgagc atcaacaaca taccacttct tctctatatc
    22801 ggaagctttc atgctatatg ttttctgaat catgccttga ttctcctgaa actatttgtt
    22861 ttacagcctg tcgaacataa aatccgctcg aaaaattacg gatgaagcga catgttactg
    22921 tttgatttac tgtatgattg ggggtataca gcaagaacac aaactaaacc cgcactctat
    22981 tatgtcaaga ttaattaact cttttgcgca aaacagtatc accaactcac tttcctgtta
    23041 taaagctata aaatttctgt tttatattcc actttatcat ccgataagca aacatataat
    23101 acaaaaaatt atattgttat aggctcacta taatttggaa tttatgtacc atttagtact
    23161 tatcagaagt tcactatttt taagtaatcg gtataagaaa atgatttatc acatacatga
    23221 agagacccca aacaagcgcc atatatcaca agctgccgat atcttgtgtt caggaggaat
    23281 actcatatac ccgacagata ctgtttattc atttggatgc gattactcaa accgggaaag
    23341 catagcaaaa ataaacagac ttatcaatag agatccaaag aaaccgcttt caattataat
    23401 taacgatatt agtgtaatgc ataattacgt gcgtcatgtg tcaaacgagg cattcaaaat
    23461 tattaaaaaa accgcacctg gtccattcac
//
LOCUS       DAOTGZ010000015        22620 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_3064
            NODE_2701_length_22620_cov_4.538843, whole genome shotgun sequence.
ACCESSION   DAOTGZ010000015 DAOTGZ010000000
VERSION     DAOTGZ010000015.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868088
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 22620)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 22620)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:22:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,785
            CDSs (total)                      :: 2,751
            Genes (coding)                    :: 2,732
            CDSs (with protein)               :: 2,732
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 12 of 19
            Pseudo Genes (internal stop)      :: 5 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22620
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2701_length_22620_cov_4.538843"
                     /isolate="AMR_MDS_3064"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..393
                     /locus_tag="P5123_02185"
     CDS             <1..393
                     /locus_tag="P5123_02185"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRX15101.1"
                     /translation="SIKREWMDIISLYSQQNHISKSHIICHVLRKHMHKLLQTPQYNM
                     HPMKSQRKGDNYVSQHIAIKPELFEKLSDIKLMYRYTLSYLLGDAMSFFDKYMSDYAE
                     SSKTINDKKRPRPRYKYVLNATISQNLH"
     gene            626..877
                     /locus_tag="P5123_02190"
     CDS             626..877
                     /locus_tag="P5123_02190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015868394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-only hydrogenase system regulator"
                     /protein_id="HRX15102.1"
                     /translation="MEERSVGVIAIIIRNRNASANAVNETLAPFADIIIGRMGLPYLE
                     RNLNIITLMVDAPTDLIGSLTGKLGMIDGVTVKSTLAKI"
     gene            892..2436
                     /gene="hydG"
                     /locus_tag="P5123_02195"
     CDS             892..2436
                     /gene="hydG"
                     /locus_tag="P5123_02195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009610104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="[FeFe] hydrogenase H-cluster radical SAM
                     maturase HydG"
                     /protein_id="HRX15103.1"
                     /translation="MNSMKTADILSVKNWKDNLVKQDEIDRYLEDGKDFIDDDAIEAE
                     IEKNRGASTERIRQIIKKAYEIKLMDQSDVAALLSVTDDTLKEEIFKAAREIKTKVYD
                     NRVVTFAPLYCGSKCVNNCKYCGFRSDNNSIERRVLTMQEIEEETRVLAGKIGHKRLV
                     VVYGEHPETDADYIAQSMKHIYSVKERVRNGSGEIRRVNINAAPLPISDLDKLRKAGI
                     GTYQVFQETYHHKTYKEVHPEGTLKGNYPWRLYALHRAMDAGIDDVAIGALFGLYDWK
                     FEIMGMVAHARDLEKRYGLGPHTVSVPRLEPANDTDFSWVKHRVSDDDFRFIIAVIRL
                     AIPYAGLIVTNRESPEMLQSVIKMCTQRDADTRVGIGAYTDAYPLEIENGGNDAQKGE
                     RQQFILGDTRSLDAIIRELAEMGHIVSFCTAGYRCGRTGKKIMDALTSGQEGCFCKLN
                     AVLTFQEWLEDFATPETKVIGDEIIEKEMDEIRARVPKDFSEAQLKHLEKARQRILAG
                     ERDIYF"
     gene            complement(2543..4201)
                     /locus_tag="P5123_02200"
     CDS             complement(2543..4201)
                     /locus_tag="P5123_02200"
                     /inference="COORDINATES: protein motif:HMM:NF021619.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PocR ligand-binding domain-containing protein"
                     /protein_id="HRX15104.1"
                     /translation="MKKLLLVAEDRIFAIDKKWSYNKHGFNLVYTDSGDHAIKTVVND
                     SSINLVLVDLSFDNGMHACDCASEILLKKNLPIVFLTGIIEDHLIQKCEQIESYGFIE
                     KYSAVSLHVAVIKAALRMHQNRKSYLNKNIFKKNGVVDFLSYKTSRDVIRSHNCSSNA
                     IMEANGVIDFETVFHIDQIQKLQNDFSDATNVASIITLPDGTPVTEPSNFCRLCEEII
                     RKTEKGLAECIRYNPIFGEKTEDSPVILTCKSGGLWDSGAPICLEGQHIANWLIGQVR
                     NGSYPEEGMREYAKHIGTDDETLIKAFKDVPTMSYEQFAKITRALNTLVDQFFSTAYS
                     NFQQACSIEAMQHNEDLLKEACNQNRILLRELQHRAKNSFALISSMITLVIDTITDEE
                     ARSALKAVSYRISAISDLYSLLYTTDLVKEVHLDEYLIKLCRSFEGMTDNICFNYSFD
                     NVITSVRTAIPVGIIVTELVTNAIKHAFPDSCSGSVWIRLIDEQNKIILEISDNGIGY
                     NSGTGQISCDTFGLKFITALVGEIDGKLTMESKNGSMVRIEFDN"
     gene            complement(4524..5582)
                     /locus_tag="P5123_02205"
     CDS             complement(4524..5582)
                     /locus_tag="P5123_02205"
                     /inference="COORDINATES: protein motif:HMM:NF024242.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HRX15105.1"
                     /translation="MAAALYVRPVSLRSVTLSIIFISFSGMQFSSVMFYTRLVYQIPG
                     IIYVTPALVALIPVALYLYTQTFTCERKRIFVSDFPHFIPFLIIAIISLFEFISPLET
                     KLQHIDKLYTDYSPFSILVVLNMIYLIVYFIICIISLHKVYLKENIVHKRIMRIFMFF
                     LSIIVLSILKVVGLVFSSVMNNGLDFMMTATVIFSIYLIYFFFLSQRFPVLLAYGSLA
                     PSHNSLFAGKVDNSKRTRTTLDAINIEHTAKEIAMLMEEEKIYCDEDLTLKRLSRAMN
                     ITPHKLSAFLNDYYGKNFNTFINSYRIKHACELLNDPSVSTLSAAFASGFNSYTAFYT
                     AFKKHTGKSPGNYKTLSK"
     gene            5786..7111
                     /locus_tag="P5123_02210"
     CDS             5786..7111
                     /locus_tag="P5123_02210"
                     /inference="COORDINATES: protein motif:HMM:NF014443.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015379 - potassium:chloride symporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TrkH family potassium uptake protein"
                     /protein_id="HRX15106.1"
                     /translation="MKQRSPKFILVVSFLILIATGTVLLSLPAATNKPISVMDALFTA
                     TSAVTVTGLIVLDTPVDFTIFGQAVILILIQMGGLGIMTFSLGLISLISRSLSIKWQF
                     TLMDIYGNNHKLPILKILKQILIFTVILEIFITVLLLPFFMMRFPFGMALWHSLFHAV
                     SSFCNAGFSTFSDSLCGFSDSYYLNIVISISIVFGGIGFLVIHDVVNNLRKKTIKKIS
                     VHTKIVLLTTILLILSGAIFFYYYEYNFILKNSPIGEKITVSFFQSITCRTAGFNTID
                     IGKLQNNTLFFMIILMFIGGSPGSIAGGIKTTTLWVIVAIIISQLRRHEQIKFFNRAV
                     SRSTAERATTLFILAMAIITTATLIVSGFEAHEGSVHFLDVLFEMGSAFGTVGLSTGI
                     TTELNTVSRIITIIVMLTGRLGPLTFLAVFSEGYITNKIKYPEEHIMIG"
     gene            7123..7815
                     /locus_tag="P5123_02215"
     CDS             7123..7815
                     /locus_tag="P5123_02215"
                     /inference="COORDINATES: protein motif:HMM:NF014325.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TrkA family potassium uptake protein"
                     /protein_id="HRX15107.1"
                     /translation="MKRCIVIGLGNFGYTTAKTLYENGIDVMAVDSHEEIVSAYKDDL
                     GVTICCDATNMEQLLSLQIKNFDAAVIAIGQNMTASILISLHLQELKIKRIIVRAISD
                     DHAKILSKLGVSDIVYPEIDTAIKTANKLMMKNVADFLHISDEYSVIEIPAPASFIDK
                     NLAELNITGNYNCQVLGIKYRKPIKDDENNEDYNVDKTIIAPHAGDIIKKHSLIIVLG
                     KKNDIIRIQKIS"
     gene            complement(7858..8817)
                     /locus_tag="P5123_02220"
     CDS             complement(7858..8817)
                     /locus_tag="P5123_02220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008591479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HRX15108.1"
                     /translation="MKVKFWGVRGSLPTPLTMDTIQNKIFRALQRVKPSDLESNETID
                     NYIDSLPVSIKGTYGGNSTCIQIIPDNGDVICIDCGTGFKNFGASLMQREFKSGQGFA
                     SVFVSHTHWDHIQGIPFFLPMFIPGNKFTFYSPFDDLQQRIEYQQDFSHFPINLEMMQ
                     AKKEFVAVPQEGEIFLNGMKVINKRMRHPGASYGYRFEENGKVFCFTADCEFNLDEMD
                     EIDSYREFFQDADVVLFDAQYTFNESINKIDWGHSSASIAIDIAARFNVKKVILYHHD
                     PDYSDDKLDNVLANARSYVAMNVGKKGVELEVELALEGAEIEI"
     gene            complement(8908..9234)
                     /locus_tag="P5123_02225"
     CDS             complement(8908..9234)
                     /locus_tag="P5123_02225"
                     /inference="COORDINATES: protein motif:HMM:NF017902.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlbF family regulator"
                     /protein_id="HRX15109.1"
                     /translation="MEECLELAGYLGSRIAENQICLRYKILSRKINSEPDKKKKIDDF
                     ENCVARINKLIKEEKAVPEELQKEYEEIASMVNSDNDLLLFIEAQKSYLALIQSLIER
                     IIPNQV"
     gene            9468..9824
                     /locus_tag="P5123_02230"
     CDS             9468..9824
                     /locus_tag="P5123_02230"
                     /inference="COORDINATES: protein motif:HMM:NF016408.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polymer-forming cytoskeletal protein"
                     /protein_id="HRX15110.1"
                     /translation="MAEVKEIIEDENKIETVIAEDIHFRGTLKFKKSLKIKGRFEGKI
                     ESEGQVIIGQEATISANIEASTILVNGSVNGKLKANKSIELYKMSSTSGDIISPDLQI
                     EKGAVFNGTCLMPEKK"
     gene            9821..10453
                     /locus_tag="P5123_02235"
     CDS             9821..10453
                     /locus_tag="P5123_02235"
                     /inference="COORDINATES: protein motif:HMM:NF024957.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane beta-barrel protein"
                     /protein_id="HRX15111.1"
                     /translation="MRPLIIFLIIISASSPLFSAGRYTVGVGGGYGVNGIIDSGFFKW
                     ENEGCYRVELSLEKNVSPHITFETGLWYTSHSFQFYEIEDSSESIDIKTNNRSEYLQI
                     PLLFKPGIPFKHTKISFPVGLNFGYLINSEIGDDYESSVQEYVNSNTVSAIAGLEFAV
                     NFSFSTDIYLGVLAEMQLVDYIKNRGEKEEDEYSYLYDMIFRSGVRYRFR"
     gene            complement(10450..10956)
                     /locus_tag="P5123_02240"
     CDS             complement(10450..10956)
                     /locus_tag="P5123_02240"
                     /inference="COORDINATES: protein motif:HMM:NF013401.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HIT domain-containing protein"
                     /protein_id="HRX15112.1"
                     /translation="MRKYLFNVDKYRYVTGDRPKVDCILCEMINGNPDITNLILYRSK
                     FNLVTLNLYPYNPGHLMIAPLRHIEELTLLSAKEAADSHKLLVFCLKVLSEEFNAHGF
                     NVGYNLGDESGASISHIHQHIVPRYKNEVGFIDVLAGDRIAVVDPLVQFEKLKIRFDN
                     ADSFLSVD"
     gene            complement(10959..11441)
                     /locus_tag="P5123_02245"
     CDS             complement(10959..11441)
                     /locus_tag="P5123_02245"
                     /inference="COORDINATES: protein motif:HMM:NF016489.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidylglycerophosphatase A"
                     /protein_id="HRX15113.1"
                     /translation="MKRVAHFFYTGFYTGYMPFAPGTFGTLLACAIYCSLYMTFGDNV
                     RFINMGLVLIAAYPAVLLGNQAERYFEAKDPQVVVLDEMMGYWISIFFFPFNLKIVLA
                     AFVIFRIFDILKPWPIGSSQQLKGGLGIMIDDYIAGVFTNAVIWVIIGFSHFTGIAIF
                     "
     gene            complement(11529..12182)
                     /gene="pgsA"
                     /locus_tag="P5123_02250"
     CDS             complement(11529..12182)
                     /gene="pgsA"
                     /locus_tag="P5123_02250"
                     /EC_number="2.7.8.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004426523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008444 -
                     CDP-diacylglycerol-glycerol-3-phosphate
                     3-phosphatidyltransferase activity [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-diacylglycerol--glycerol-3-phosphate
                     3-phosphatidyltransferase"
                     /protein_id="HRX15114.1"
                     /translation="MNIPNLISMSRIFLIPVFLYLIFQPEWSYKFWALIVFGVASITD
                     FFDGWSARKLKLESEFGKFLDPLADKFLVIAALICFLILDPLIPLWMVLVIIGRDLLI
                     TFIRYLAVRKGNSIRTSHFGKVKTAFQMVSIPVIIVVFIAKSTGLDYKAIPTDQTTFL
                     TAVDFMMSGELNKIMVSLPYWLMFIITMLTGISGLRYICSNGSVLLPPYKFKNKLTD"
     gene            complement(12179..13465)
                     /gene="rimO"
                     /locus_tag="P5123_02255"
     CDS             complement(12179..13465)
                     /gene="rimO"
                     /locus_tag="P5123_02255"
                     /EC_number="2.8.4.4"
                     /inference="COORDINATES: protein motif:HMM:TIGR00089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008172 - S-methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016782 - transferase activity,
                     transferring sulphur-containing groups [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0018339 -
                     peptidyl-L-beta-methylthioaspartic acid biosynthetic
                     process from peptidyl-aspartic acid [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S12 methylthiotransferase
                     RimO"
                     /protein_id="HRX15115.1"
                     /translation="MTYHIISLGCFKNQADSETANSYLVSYGFLPVDSPEEADIIIIN
                     TCGFIEDAKKESISVILDASDITQGDGYSSNKKLVVAGCLSQRYMCDLKKDIPEVDFF
                     YGVIDGDFVPAMCEALSLKYLGGSAPKLLRVPLIASPYEYIKIAEGCSNNCSYCAIPL
                     IRGKHVSYPPEQILTDVRQAIERGAMELIIIAQDTAVYNYGSDNIVTLLRKISAIAEN
                     RWIRLLYLHPDHVTDQLLELFNELPNLVPYIEIPFQHVASGIVSSMNRKGSVETYTEL
                     LNKIRRIDPRIAIRSTFIAGYPGETDEQFEELLSFVRESNIDRGGVFIYSPEENTPAA
                     ELDNIPYELKVERYNQLSEELESGFLTALERRIGETVPVLVEEQTGEESWIGRSWYDA
                     PEVDGVFFLTGKGKLLNSIVRASVTECINFDLVGKI"
     gene            complement(13471..14616)
                     /locus_tag="P5123_02260"
     CDS             complement(13471..14616)
                     /locus_tag="P5123_02260"
                     /inference="COORDINATES: protein motif:HMM:NF024252.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HRX15116.1"
                     /translation="MESIGEKFRSLREARNLTIRMVCNDTNISPHYLEALENEDFDHF
                     PSETYITGFIRSYAEYLKTDPEEIIRAYKGYKIGESVTPLEELTRPTSSGLDINSEAF
                     AKGLKYFVIILFGVLIIGGGSFAVKNFMSSKIDVTRDDSIDEIKEEEAEESSKQSEHE
                     QIRNLKLSGNKGFVLAYTSEAVKFLVDSKECTFVVESVGVDEAVVEILPVKQKVTLLL
                     DRSIPVTFENTSREVYLTLRGYTENRAKIMVTLGGKLEDDSALADGEEVSDPKEALAV
                     STQVRATDKSNLKIVFEAIFLSKTYLEIYLDGNMKTRGIIQAGAREVWEANEYIQIKI
                     GNAGGMKAKINGELYEFGLAGQVANKVITWKKDPNDPNLYNIVVNDW"
     gene            complement(14623..15312)
                     /locus_tag="P5123_02265"
     CDS             complement(14623..15312)
                     /locus_tag="P5123_02265"
                     /inference="COORDINATES: protein motif:HMM:NF015510.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane lipoprotein carrier protein LolA"
                     /protein_id="HRX15117.1"
                     /translation="MRKYIWCAAIAVVFFTGISVNAQKFEFVTVSDVVRNVRDRFSKV
                     EGYQADFTMVTLKGSGSVTQKGVIQSLQPNYLRIMFNAPRNQQVVSDGKMMWVYIPSM
                     NVVAEQDLKDDKGTEFSAVSYVGLRRLFAKYHYKFASKEQPALMSDGKKYYTLLLQQK
                     ESRSGYKTINLWINEDYLITRAEGETSTGKKITISFDNINTNAKLLKGNFKFDIPSNA
                     KVIKNPMISEE"
     gene            15891..16079
                     /locus_tag="P5123_02270"
     CDS             15891..16079
                     /locus_tag="P5123_02270"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRX15118.1"
                     /translation="MQTKIVTRLSMPICDIEEMAFDEEIALFAEKHNLLESEIIYFSA
                     GESKIKEVLIKHEPSLSV"
     gene            complement(16128..16598)
                     /locus_tag="P5123_02275"
     CDS             complement(16128..16598)
                     /locus_tag="P5123_02275"
                     /inference="COORDINATES: protein motif:HMM:NF024258.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YfcE family phosphodiesterase"
                     /protein_id="HRX15119.1"
                     /translation="MGKVLVVSDSHGNCRALEAIIDAEPDFEVLVYCGDGIKDIAFLP
                     LRESVTVVAVPGNIDRFSPMYNEELVITNIFGVMTAVTHGHLYGVKHTMGQLILMARQ
                     LDASLVLFGHTHEPVVIEHENRMFVNPGVSGAGQYAVLEITNKVASTKLKKVGL"
     gene            16858..18654
                     /gene="lepA"
                     /locus_tag="P5123_02280"
     CDS             16858..18654
                     /gene="lepA"
                     /locus_tag="P5123_02280"
                     /EC_number="3.6.5.n1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015769439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation elongation factor 4"
                     /protein_id="HRX15120.1"
                     /translation="MKKNNIRNFSIIAHIDHGKSTLADRIIDSCTPIKSRIKVDQMLD
                     TMDLEKERGITIKSNAISLVYKAINGEDYVFNLIDTPGHVDFTYEVSRSLAACDGVLL
                     VVDASQGVEAQTIANMYMALENNLEIMPVINKIDMPAADIDRTKESIEKTLGLDPEDA
                     IAVSAKEGINIDMLLEAIVEKIPAPQGDENKPLQALIFDSYFDQYVGAVAKIRVFEGK
                     ISTNDDIIFMSNDKKFTVNEIGVFGFKPEKRPSLKAGDVGYLIANIKSVTDTQIGDTV
                     THVSNRCEKPLSGFKEAKSMVFAGLYPMFSDDYENLREALQKLQLNDASLLFEPDNSY
                     ALGFGFRCGFLGLLHMEIIQERLEREFDLSLVTTAPSVEYHVKTIQGEEMIIDNPVKF
                     PEPGSIEYIKEPYVRTTIITPKEFIGNLMNLSIDCRGIHKEMKYIDENRVEIINDMPL
                     AEIVFNFYDKLKSLSKGYASFDYELIDYRESKMVKADILVNGESVDALSFIVHTDKAY
                     QRGKDIIEKLKDIIPRHQFKIPIQAAIGAKIIARETINALRKNVTAKCYGGDISRKRK
                     LLEKQKEGKKRMKLIGSVEIPQEAFLSILRID"
     gene            complement(18786..19712)
                     /locus_tag="P5123_02285"
     CDS             complement(18786..19712)
                     /locus_tag="P5123_02285"
                     /inference="COORDINATES: protein motif:HMM:NF025598.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4340 domain-containing protein"
                     /protein_id="HRX15121.1"
                     /translation="MNKKKLYSILTIVVLLLLIFGKGFFSGVSLPEFKKWEEDPKIVT
                     IYRPGGDVLKLEKKDDKWYINNTDMVASETEAETIVSKLKTLKIFDRVSSSDDASYRY
                     ELDEEQAIRVTIESANNKLFEIMVGKQSTGMNQSYVKFVDDSSIYLAGTITPRDLRKS
                     VFEMRSKEIARFSYRDIESFSVSYEGSSFTLLPYDEEVEAPDGTVQNERRWKFKTGSE
                     KVNMNSAIAMMNAVNPFIADQLSEEAIEDVSDPLGVMEVVINGKTVTINIVKKDDQKY
                     YYLKRTDSDDVYKLYETRVNHIFRKKEALIAR"
     gene            complement(19712..21835)
                     /locus_tag="P5123_02290"
     CDS             complement(19712..21835)
                     /locus_tag="P5123_02290"
                     /inference="COORDINATES: protein motif:HMM:NF021351.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gldg family protein"
                     /protein_id="HRX15122.1"
                     /translation="MAVRESITNAMRDMLVSVKNFFVTDAYNYVFYFIILILLNVFAS
                     SYNLRFDLTDGGVYSLSDVSKRSVKNITEPLSVRVFFSEDLPAPYSNVRLYLKDILEE
                     YKSAADGNFTFEFVDMSKDEGKNIARSYGIQPIEIRQLSGDEYKAKSAYMGMVFIHGD
                     IIDKIPQVARLKGLEYMITTKITKMRGKVDVLSGSESVKVTLYVSDKLEMFNINGLNK
                     LETAVKEVVDSASKENSGKVEFSSVSSVDDQTIEMLVSKFGATPLSWEDTRLPDGRRL
                     AAGQGVITVTLEYGEKKSAINLQVAQTPFGMMLSGVEELDENVKSTIDSLISSNMVIG
                     YVVTGATKDVQDEQAGGALLNLLSDMYEIQMIDLAQGAIPETISTVIINGPVLPLAPA
                     ELYAIDNYIMRGGNVLFFTDMFYEIPQNRQQQQMQQPPQFRPIENGIPGLLETYGVKV
                     NQDYVMDMQSFVMQQPGMDPVNLYFAPEIFRENLNKDNPSTSYLNYMLMVKASSIELD
                     KQKLESKNIKSEVLVSSSPESWQMTGEINLSPYGLEIPEKDAMESYPLAVILDGKFES
                     HFANGKPDDGEKKESSSLMVEKHLKESISNSKIAVVSTSEITSGNLQNINNNDPRQMF
                     VYNNALFLHNLVDYMSGFDDVPQMRSKGLESNLKEVSETSKNVFKIINIAALPLLSVI
                     MVLVIIRLRMMRRKKIERRYAKEVK"
     gene            complement(21837..22574)
                     /locus_tag="P5123_02295"
     CDS             complement(21837..22574)
                     /locus_tag="P5123_02295"
                     /inference="COORDINATES: protein motif:HMM:NF024091.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HRX15123.1"
                     /translation="MNNNMINFKYSFRQTRALFGKELRSYVGSPILYIIMTVFLLLTG
                     VFYFKDFYIIGRADMRQFFNVLPFFFMFFIPAITMRQIAEERSSGTLEMIITMPVSAS
                     NLVLGKFFASVAIVMIMMAPTLFYVLSLGVAGVPDYGMIVAGYIGTIFLAASYVAVGI
                     FASSLTKNQIIAWLVSTAFCVFFTMIDYFIQFIPGKIFAFLNYLSASYHFQGLAKGVL
                     EFRNITYLITVSLFFIILSVRSIEERR"
BASE COUNT         7478 a         4472 c         3856 g         6814 t
ORIGIN      
        1 agcataaaaa gagagtggat ggatataatc agtttatatt cgcaacagaa ccatatttca
       61 aaatcacata taatttgcca tgttttgcga aaacacatgc ataagctatt acaaacgcct
      121 caatataata tgcacccaat gaaatcacaa agaaaaggcg acaactatgt atcccaacat
      181 attgcgatta aaccagaatt atttgaaaaa ttatcggata taaaattaat gtatcgatat
      241 acgttgtcct atcttcttgg agacgcgatg agcttttttg ataagtatat gtctgattat
      301 gctgaatctt caaaaaccat caatgataag aaaagaccaa gaccaagata caaatatgta
      361 cttaatgcaa ctatttctca aaatttacac taattgccaa taattaaaat ttattatata
      421 tttctccata tcttattgat tttattaaat actattttat aatgtcaaaa tataacctgc
      481 ggtcaggatg aagtaatttc tgattctgaa gccaagggcc ctatgtacct ctttttgttt
      541 gcctacatgc ttaaatcagg catttttaag caattaaaac cgtacaaatg gctcctccgc
      601 agaatttctt aatggaggga tttacatgga agaacgctct gtaggcgtaa tagcaattat
      661 tatccgcaac agaaatgctt ctgctaacgc tgttaacgaa acattggcac cttttgcaga
      721 tatcataatc ggcagaatgg gcctacctta cctcgaacgt aatctcaata tcataacact
      781 aatggtagat gcaccaaccg acctaattgg ttctcttacc ggtaaactgg gcatgataga
      841 tggtgttacc gtaaaatcga cattggcaaa gatataacac aggagacaat catgaatagt
      901 atgaaaacag ctgatatttt atctgtaaaa aattggaaag ataatcttgt taaacaggac
      961 gaaatagatc gttatcttga agacggcaag gattttattg acgatgatgc cattgaagca
     1021 gaaattgaaa aaaatagggg cgcctctacc gaaaggattc gtcaaattat taaaaaagct
     1081 tatgaaatca aacttatgga tcagtccgat gttgcggctc tcttatctgt aaccgatgat
     1141 acgctaaaag aagaaatttt taaagctgcc cgcgagatca aaactaaggt ttatgataac
     1201 agggtggtaa cctttgctcc tctctattgc ggaagtaaat gtgtaaataa ctgtaaatac
     1261 tgcggtttca ggagtgacaa taactcaata gaacgacgtg ttttgaccat gcaagagata
     1321 gaagaggaaa caagagttct tgccggcaag atcggccata agagacttgt tgttgtatat
     1381 ggcgaacatc cggaaacaga cgcagattat attgcacaaa gcatgaagca tatatattct
     1441 gtcaaagagc gagttagaaa cggctcaggt gaaatacgcc gtgttaacat taatgcggca
     1501 ccgttgccga tttctgacct tgacaaactt cgaaaagctg gaataggaac ctatcaggta
     1561 tttcaagaga cttatcatca taagacatac aaagaggtgc atcccgaagg gacattgaag
     1621 ggcaactatc cctggagact ttacgcactg caccgagcta tggatgccgg aattgacgat
     1681 gtcgcaatag gcgccctttt tggtttgtat gactggaaat ttgaaataat gggtatggtg
     1741 gcacacgccc gggatcttga aaagcgatat ggcctaggac ctcatactgt ttccgtacca
     1801 cgccttgaac ctgcgaacga taccgacttt tcttgggtta aacacagagt cagcgatgac
     1861 gatttccgtt ttataattgc ggtaatcaga ctggcgattc cttacgccgg actgattgta
     1921 acaaaccgag aaagccccga aatgttgcaa tctgtaatta aaatgtgtac acaacgtgat
     1981 gctgacacac gtgttggaat cggagcatat acagatgcct atcctttaga gatcgaaaac
     2041 gggggaaatg acgcgcaaaa aggcgaacgc caacagttta ttcttggaga tacccgaagc
     2101 cttgacgcaa ttataagaga gctcgcagaa atgggtcata tagtatcctt ctgtacagcc
     2161 ggataccgct gcggccgaac cggcaagaag ataatggacg cgcttacctc cgggcaggag
     2221 ggatgttttt gtaaactgaa tgcggtttta acatttcagg agtggctcga agatttcgcg
     2281 actccggaga ctaaagttat tggcgatgaa attatcgaaa aagagatgga tgagatcaga
     2341 gcacgtgttc ctaaagattt ttcagaagct caattaaaac atcttgagaa agcgagacaa
     2401 agaatattgg caggtgagcg tgatatctat ttttagatat tcattgtttt ttgttttgta
     2461 aacaactatt acttaaaaga ttatacgcat gctaataaat catacccggt atcacatcaa
     2521 ccgataccgg gttaaaatct gtttaattat caaattcgat tctaaccata ctaccgtttt
     2581 ttgattccat ggttaactta ccatcaattt cgccaacaag agctgtaata aactttaatc
     2641 caaaagtatc acatgaaatt tgccctgttc cagaattata accaattcca ttatccgata
     2701 tctcaagaat aatcttgttt tgctcatcaa taaggcgtat ccataccgaa cccgaacaac
     2761 tatccggaaa agcatgctta attgcattgg taacaagttc tgttacaata ataccaaccg
     2821 gtattgcagt tcgaacggaa gttatcacat tatcaaatga atagttaaaa caaatattat
     2881 ctgtcattcc ttcgaaagac ctgcaaagct tgattagata ttcatcaaga tgaacttctt
     2941 taacaagatc tgttgtgtac aatagtgagt ataaatccga aattgcactt atacgataac
     3001 ttacggcttt taaagcacta cgcgcctctt cgtccgttat cgtatctata accaaagtaa
     3061 tcatgcttga aattaacgcg aagctattct tggctctatg ctgaagttct ctaagaagta
     3121 ttctattttg attgcaagct tccttgagta agtcttcgtt atgttgcatg gcttctattg
     3181 aacaagcttg ttggaaattg ctgtaagctg ttgagaagaa ctggtcaaca agtgtattta
     3241 gcgcacgagt tattttcgca aattgttcat aagacattgt tggaacatct ttgaatgctt
     3301 ttattagagt ttcatcgtct gttccaatat gtttagcata ttcgcgcatg ccctcctcag
     3361 gatagctacc attgcgaacc tgtccgataa gccagttagc aatatgctgc ccctcaagac
     3421 atattggtgc accagaatcc caaagaccgc cacttttaca tgtcagtatt acaggagaat
     3481 cttccgtctt ctctccaaaa ataggattat aacgtataca ttcagcaaga cccttttcag
     3541 ttttacgaat gatctcttca cataacctgc aaaaattact tggctcggta acaggagttc
     3601 cgtctggcaa ggtaatgata gatgcaacat ttgttgcgtc cgaaaaatca ttctgaagtt
     3661 tttgtatctg gtcgatatgg aatactgttt caaagtcaat aacgccatta gcctccatta
     3721 tagcgttaga agagcagtta tgacttctta taacatcacg tgaggtctta tatgacaaga
     3781 aatctacaac tccatttttt ttgaaaatat ttttatttaa atagcttttt ctattttgat
     3841 gcattcttaa agccgctttt atgaccgcca catgaagtga tacagccgag tacttctcga
     3901 taaatccgta agattctatt tgctcgcatt tttgaataag atgatcttct atgatgccgg
     3961 ttaagaatac aataggtaga ttttttttta acaatatttc tgatgcgcaa tcacaggcgt
     4021 gcatcccatt atcgaaggat aagtcgacaa gaacaaggtt tatagagcta tcatttacta
     4081 ctgtttttat tgcatgatca cccgaatctg tatagaccaa attaaatccg tgtttattgt
     4141 aactccattt tttgtctatc gcgaaaatcc ggtcttcggc aacaagaagc aattttttca
     4201 taatcagtat cctgatggca accggacaca cgcgataagt ccgatatagt tttatttttt
     4261 ataatattta atatttgata tttgatattt gatatttgat aatttaaaga aaaatgacat
     4321 atttctttta tagcacttta ttcataatta tctaaataga tgaacctatt ttaatcattt
     4381 tttttaattt tgtcaataaa agaattattt tttgctatat aaatttttag caaaattaaa
     4441 caaatatctt tacctgatta acaagcaaag cttatttgaa gtaaatcttt tgattcatct
     4501 gataagcaat ttacaatttt tgattattta gacaatgttt tatagttacc gggagatttt
     4561 ccggtatgct ttttaaaggc cgtataaaaa gctgtataag agttaaaacc agaagcgaat
     4621 gccgcggaca atgtactaac tgaaggatca ttaagtaatt cacacgcatg ctttattcta
     4681 taactgttta tgaatgtatt aaaatttttc ccataataat catttaaaaa agcggacaac
     4741 ttatgaggag taatattcat agctcgagat agccttttta aagttaggtc ttcatcgcaa
     4801 taaatttttt cctcctccat aagcatagct atctccttag ctgtatgctc tatattaatt
     4861 gcatcaagag tagttcttgt tctttttgag ttatcaacct taccggcaaa caatgaattg
     4921 tggcttggtg caagagaacc gtatgccaat aaaaccggaa aacgctgtga aagaaaaaaa
     4981 aagtaaatca ggtaaataga gaagattaca gttgcggtca tcatgaaatc cagtccgtta
     5041 ttcataaccg atgaaaagac cagaccgacc acttttaata ttgataaaac aattatacta
     5101 agaaaaaaca tgaaaattct cattattcgt ttgtgcacga tattttcttt tagatatacc
     5161 ttgtgaagac tgattataca tataataaaa tagactatca ggtaaatcat attaagtaca
     5221 acaagaattg aaaaaggtga ataatcagta tacagtttat caatatgctg caattttgtt
     5281 tccaacggag agataaattc aaacaacgat ataatagcga ttatcagaaa cgggataaag
     5341 tgcggaaagt cagatacaaa aatacgtttg cgttcacagg taaaggtctg cgtgtataaa
     5401 tagagtgcaa ccgggatcag agccacaagg gctggagtta cgtatattat acccggaatt
     5461 tgataaacaa gccgcgtata aaacatcaca ctcgaaaact gcattccgga aaaagagata
     5521 aaaataatac ttaaagtaac cgaacgtaac gacacaggcc gcacataaag tgctgccgcc
     5581 attaataagc ccaagcctac ggtaaacaat acaaaataag attcgattcc cattcactta
     5641 ctttaaaact gcacttaaaa acgtcaataa atatcctgtt caaattacct gtaatatttc
     5701 tctttaccag aataaaaaga aagtttttaa tacataatta atgaaaaatc gttgaagcct
     5761 agtgtgcaat agatagcatg tgccaatgaa acagagatcc ccaaaattta ttcttgtagt
     5821 tagctttctg attcttatcg ccactggtac ggtactactc tccttgcctg ccgcaacaaa
     5881 taaaccgatt tccgttatgg atgcactttt taccgcaacc tcggctgtta cagtcaccgg
     5941 attgatagta cttgatacac ccgtcgactt tacgatcttt ggacaggcgg taatactgat
     6001 tctgatacaa atgggcggcc ttggcataat gaccttttca cttgggttaa tttcacttat
     6061 aagcagatcc ctgtcaataa aatggcaatt cactttgatg gatatatacg gaaacaatca
     6121 taaactaccc atacttaaga ttttaaagca gatactgatt ttcaccgtta tacttgagat
     6181 atttattacg gttttacttc tgcctttttt tatgatgagg ttcccctttg gaatggctct
     6241 ttggcattca ctttttcatg cagtttcatc tttctgtaat gccgggtttt cgacattttc
     6301 tgattcttta tgcggctttt cggacagcta ctacctaaat atagttatct cgatatcaat
     6361 agttttcgga ggtatcggat ttttagtaat acacgatgtg gttaataatc taagaaaaaa
     6421 aactatcaaa aaaatttcag tacatacgaa gattgtttta ctaacaacaa tattattgat
     6481 cctttcaggg gcaatattct tttactatta tgagtataac tttatcctga aaaacagtcc
     6541 cattggtgaa aaaataactg tttcattttt ccaatcaata acctgccgca ccgcaggctt
     6601 caatactatt gatataggga aacttcagaa taacaccctt ttcttcatga ttatattaat
     6661 gtttataggt ggctctcccg gctcaatagc aggtggaatt aaaaccacca ccctttgggt
     6721 aattgtggca atcataatct ctcagcttcg tagacacgaa cagataaaat tctttaaccg
     6781 tgctgtttca cgatcgactg ccgaaagagc tacaaccctg tttatattgg ccatggcaat
     6841 aattacaaca gcaactctta tagtatccgg atttgaagct catgaaggaa gtgtacattt
     6901 tctggatgtt cttttcgaaa tgggttcggc ctttggcact gttggacttt caaccggaat
     6961 aactacagaa ttgaatacgg ttagccgaat tattacaatt atagtaatgc tcactggccg
     7021 tcttggtcct ttgacctttc ttgctgtttt tagtgaaggt tacataacca acaaaataaa
     7081 atatccggaa gagcatatca tgattggtta aggagaattt gaatgaaaag atgcatcgta
     7141 ataggacttg gtaactttgg ctatacaacc gctaaaacac tgtacgaaaa cggtattgac
     7201 gttatggctg ttgactctca cgaagagatc gtaagcgcat acaaggatga cctgggtgtg
     7261 accatttgct gtgacgccac taacatggag cagcttcttt ctcttcagat taaaaacttt
     7321 gacgcggcag tcatagctat cgggcaaaac atgactgcaa gcatactgat ctctctacat
     7381 ttacaggagc tcaaaataaa gcggataata gtgagggcaa tatccgatga tcacgcgaag
     7441 attctttcga agcttggagt cagcgatatt gtttatcccg aaatagacac tgcaattaaa
     7501 acagccaaca aactaatgat gaaaaacgtc gccgattttt tacatatatc tgatgaatac
     7561 agtgttattg aaatccctgc accggcaagc tttattgata aaaatctagc agaacttaat
     7621 attacaggta attataactg ccaagtgtta ggcataaaat atcgtaagcc gattaaggat
     7681 gacgagaata acgaagacta taatgttgat aaaacaataa ttgctcctca tgctggtgac
     7741 attatcaaaa aacattccct tattattgtt cttggtaaaa aaaacgatat aatcagaatt
     7801 caaaaaataa gttgatcact tttttaagca ataaatgaca ttcaatcatg atttctatca
     7861 aatttcaatt tccgcaccct ctagggcaag ctcaacctca agctcaaccc ccttcttgcc
     7921 aacattcatt gcgacatagc ttcttgcgtt agccaaaaca ttatccaact tatcatcgct
     7981 gtaatcagga tcatggtgat aaagaatgac tttttttaca ttgaaacgag cggcaatatc
     8041 tatcgcaatt gaagctgacg agtgccccca atctatttta ttgattgact cgttaaatgt
     8101 atactgggca tcaaaaagaa caacatcggc atcttggaaa aattcacgat aactgtcaat
     8161 ttcatccatt tcatcaagat taaattcaca atctgccgta aagcagaata ctttgccatt
     8221 ttcttcaaac ctgtagccat acgaggcgcc gggatggcgc atgcgtttgt taatcacctt
     8281 cataccgttt aagaatattt ccccctcttg gggaacagca acaaactcct tttttgcctg
     8341 catcatctca agattaatgg gaaaatgtga aaaatcctgt tgatattcaa ttcgctgttg
     8401 taaatcatcg aaaggagaat aaaaggtaaa tttgttaccc ggaataaaca tcggtaaaaa
     8461 aaacggaatc ccctgaatat gatcccaatg tgtatgtgaa acaaaaacgg aagcaaatcc
     8521 ctgcccgctt ttgaattcac gctgcattaa agaggcgcca aaatttttga atcctgtacc
     8581 acaatcgata cagataacat ctccgttatc tggaataatc tgaatacatg tagagtttcc
     8641 accataagtg ccttttatcg acacaggaag ggaatctata taattatcaa tagtttcatt
     8701 tgactcaaga tctgatggtt ttacccgttg taaggccctg aagattttgt tttgtattgt
     8761 atccattgtt aatggagttg gcaatgatcc ccgaactccc caaaatttca ctttcatcgc
     8821 tatctgcctc tgtttagcat tgttcctata ctgattttcg gcagttatcc ccatacagaa
     8881 aagaaagaaa atatgcttaa tctattgtta tacctgatta ggaataattc tttcgattag
     8941 gctctgaatc agagccaaat aacttttttg ggcttcaatg aaaagcaaaa gatcattatc
     9001 agaatttacc attgaagcaa tttcctcata ttccttttgc agctcttctg gtacggcttt
     9061 ctcttccttg ataagtttat ttatacgggc aacacaattc tcaaaatcat ctatcttctt
     9121 ttttttatct ggttcgctat taatcttgcg agacagaatt ttatatctta agcaaatctg
     9181 attctcagca attctggaac ccaaataccc agcaagctcc aaacattctt ccatattaac
     9241 tccatttttt tgtttttttt tacaaaatga caactttgca caataattta gaacaattct
     9301 tatgaaaagg cacgcaatac cgtggtcaat aattatttcc actttgttta ataatcttgc
     9361 atattctgat tattgtccta agtttgtctt cttacgataa tctgtcttga caaaaatcaa
     9421 cagatagata ttttttaact aatagagata aattagcgga gaataatatg gccgaagtta
     9481 aagaaatcat tgaagatgaa aacaagattg agacagttat tgcggaagat atacattttc
     9541 ggggcacact gaagtttaaa aaatccctaa aaataaaggg tcggttcgaa gggaaaatag
     9601 aatctgaagg tcaggtaata atcggacagg aagcaacaat tagcgcaaac attgaagctt
     9661 cgacaatact tgtaaacggc tcagttaacg gaaagttaaa agccaataaa agcatagaac
     9721 tttataaaat gagttcaacc tctggcgaca ttatttctcc ggatcttcaa atagaaaaag
     9781 gtgctgtttt taacggcacg tgtttaatgc ccgaaaaaaa atgagaccgt taattatatt
     9841 tttaatcatt atttccgcct cttcaccact cttttccgcc ggccgctata cggttggtgt
     9901 aggtggtggt tatggagtta acggtataat cgattctggt ttttttaaat gggagaatga
     9961 ggggtgctac agagtagagt tatccctaga aaaaaatgtt tctccacata tcacgtttga
    10021 gaccggttta tggtatactt cacactcttt tcaattttat gaaattgaag atagcagcga
    10081 gagtattgac ataaaaacaa acaacagatc agaatatcta caaatccccc ttctgtttaa
    10141 acctggaatt ccttttaaac acaccaagat ttcgtttccc gttggcttga atttcggtta
    10201 tcttattaat tcggaaatag gtgacgatta cgaatctagc gttcaggaat atgttaacag
    10261 taatacggta tcagcaatag caggtttgga atttgcagtt aacttttcat tttccacgga
    10321 tatttattta ggagttcttg ccgaaatgca gctggttgac tatataaaaa accgcggcga
    10381 aaaggaagaa gatgagtatt cttatcttta tgatatgata tttcgaagcg gcgtccggta
    10441 cagatttcgt taatctaccg ataagaaaga gtcggcatta tcaaaacgta tttttaactt
    10501 ctcaaattga actaacggat ccacaacggc aatacgatca cccgccaaaa cgtcaatgaa
    10561 tccgacctca tttttgtatc tgggaacaat atgctgatgt atatgggata tacttgcccc
    10621 actttcgtct cccagattat accccacgtt aaaaccgtgg gcattaaact cttcactaag
    10681 aactttcaaa cagaaaacca aaagcttatg agaatccgca gcctctttgg cacttaatag
    10741 tgttaactct tcaatatgcc gtagaggcgc tatcatcaaa tgcccgggat tataaggata
    10801 caaattcaat gttacaagat taaatttgct tcgataaagt ataaggtttg taatatccgg
    10861 atttccattg atcatctcgc acagaataca atcgacctta ggcctatcac cagtaacata
    10921 cctgtattta tcgacgttaa aaagatattt tctcataatc agaaaattgc tattccagta
    10981 aagtgtgaaa atccaataat cacccatata acagcattgg taaatacacc agcaatataa
    11041 tcgtcaatca tgattcccaa accacctttc aactgctgag aactgccaat aggccaaggc
    11101 ttcaaaatat caaaaattct aaatatcaca aatgctgcca agactatctt aagattaaac
    11161 gggaaaaaga aaatcgagat ccaatacccc atcatctcgt ccaataccac tacttgcggg
    11221 tcttttgctt cgaaataacg ttctgcctga ttgccaagca ataccgcagg ataagcagct
    11281 attaaaacaa gccccatatt aataaaacga acattatcac caaaagtcat ataaagagaa
    11341 cagtagatag cacaagccaa taatgttccg aaggtacccg gagcaaaagg catatatccg
    11401 gtgtaaaagc cggtataaaa aaaatgagct actcgtttca ttgttatctc ctataatttc
    11461 atattaaatc aaaatagact atgaataata ttaacattca ttgtctaaaa aaaataaagt
    11521 gcttaaaatc aatctgttaa cttattttta aatttataag gcggcaaaag tacagaaccg
    11581 tttgaacaaa tatatcgaag acctgaaata ccggttaaca ttgtaataat aaacataagc
    11641 cagtaaggca aagagaccat aatcttgttt aactcacctg acatcataaa atccactgca
    11701 gtcaggaatg tagtctgatc agtcgggata gccttatagt caagacctgt actcttcgca
    11761 ataaagacaa caatgatgac tggaatagaa accatttgaa aagcagtttt aacttttccg
    11821 aaatgagaag tcctgatgga attgcctttt cgtacggcaa gatagcggat aaatgtaatc
    11881 agtaaatcac gaccaataat aacaagaacc atccaaagcg gtattaaagg gtccaaaatc
    11941 aggaaacata tcaaagccgc gattactaaa aacttgtctg cgagcggatc aagaaatttt
    12001 ccgaattcag attcaagttt caatttacga gcactccagc catcaaaaaa atcggtgatt
    12061 gaagcaactc cgaaaacgat caaggcccag aacttgtaac tccattcagg ttgaaaaata
    12121 agataaagga aaactggtat taaaaaaatt cgagacattg aaatcagatt aggtatattc
    12181 atattttacc gaccagatca aaatttatac attctgtaac tgatgctctc actatagaat
    12241 ttagcaattt tcccttgccg gtcaagaaaa aaactccatc cacctcaggt gcatcgtacc
    12301 aacttcttcc aatccagctc tcttcccctg tctgttcttc aacaagaaca ggtacagtct
    12361 caccaatccg acgctctaac gccgttaaaa atcccgactc gagctcttca gagagttggt
    12421 tataacgctc gacctttaat tcgtagggaa tattatccaa ttcagccgcg ggggtgtttt
    12481 cttcaggaga atatatgaaa actccgcctc ggtcaatatt cgattctcga acaaacgata
    12541 acagctcttc aaactgttca tcagtttctc cgggataacc cgcaataaat gtagaacgta
    12601 tggcaatacg agggtctatt cggcgtattt tattcagtaa ttcagtgtat gtttctaccg
    12661 agccttttcg attcatagaa gaaacaatgc cagaagcaac atgttgaaac ggtatctcga
    12721 tatagggaac cagatttggt aactcattaa ataattcaag aagctgatca gttacatgat
    12781 caggatgcag atataaaaga cgaatccagc gattttcggc tatagctgat atttttcgca
    12841 aaagtgtaac tatattatcg gaaccgtaat tatatactgc tgtatcctga gcaataataa
    12901 tcagttccat ggcaccacgt tcaatagcct gacgcacatc cgtaagtatc tgttcgggtg
    12961 gatatgagac gtgttttcca cgtataagcg gtatcgcgca atatgaacag ttgtttgaac
    13021 agccttcagc aattttaata tattcgtagg gtgaagcaat taatggaacg cgaagcaatt
    13081 ttggagccga accgcctaag tattttaaag agagtgcctc gcacatagcc ggtacaaaat
    13141 cgccatctat tacaccataa aagaaatcga cttcaggaat atctttttta agatcgcaca
    13201 tataacgttg tgagagacaa cccgcaacga caagcttttt gtttgaagaa tagccgtcgc
    13261 cctgtgtaat atcggatgca tcaagaatta cagatatgga ctcttttttt gcgtcttcaa
    13321 taaagccaca tgtattaata attattatat ctgcttcctc aggagaatca accggtaaaa
    13381 aaccgtatga aacaagatag gaattggctg tttcagaatc tgcctgattt ttgaaacagc
    13441 ccagagagat aatatggtag gtcaattatt tcaccaatcg ttaacaacaa tattgtataa
    13501 attaggatca tttggatcct ttttccaagt tataaccttg tttgcaacct gaccggcaag
    13561 tccaaactca taaagctctc cattgatctt tgccttcatt ccgccagcat taccgatttt
    13621 gatttgtatg tactcattag cttcccacac ttcacgtgct ccagcctgga taatacctct
    13681 ggttttcata ttgccatcga gatatatttc aagatatgtt ttactaagaa atatagcctc
    13741 aaacactatt tttaaattcg atttgtcagt agcccgaacc tgagtactta cagccaaagc
    13801 ttctttagga tcagaaacct cttcaccatc ggcaagagca ctatcgtctt caagcttacc
    13861 acctaaagtc accattattt tagcacggtt ttcggtataa cctcttaacg tcagataaac
    13921 ttctcgggaa gtattttcaa aagtaacagg aatcgagcga tctaatagaa gagtcacttt
    13981 ctgtttaaca ggaagtatct cgacaacagc ttcatcaaca cctactgact caacaacaaa
    14041 tgtacattct ttagaatcga caagaaactt aacagcttca cttgtatatg caagaacaaa
    14101 tcccttatta ccacttaatt ttaaatttcg aatctgctcg tgttcagatt gcttactact
    14161 ctcttcggct tcttcttctt taatttcatc aatagaatca tcgcgtgtta catcaatttt
    14221 tgacgacata aaattcttta ccgcgaatga tcctccccca ataattaaaa caccaaaaag
    14281 tattattaca aaatacttta aaccctttgc aaaggcttct gaattaatat ccagccccga
    14341 gctggtaggt cttgttaact cttctaaagg agttacagac tcaccgatct tgtaaccttt
    14401 atatgccctg atgatctctt caggatcagt ttttaaatat tcggcataac ttcggataaa
    14461 ccctgtaata tatgtttcac taggaaaatg gtcaaaatct tcattttcaa gtgcctcaag
    14521 ataatgagga gaaatattgg tgtcgttgca caccatacgt attgttaggt ttcttgcttc
    14581 ccggagcgaa cggaattttt cgccaatact ttccatacgg atctactcct ctgatatcat
    14641 tggatttttt ataaccttgg cattcgaagg gatatcaaac ttaaagtttc ctttaagcag
    14701 ttttgcattt gtattaatat tatcaaacga tattgtgatt tttttccctg ttgaggtttc
    14761 accctcggcg cgagttatca ggtaatcttc gttaatccag agattaatgg ttttataacc
    14821 gcttcgactc tctttttgtt gaagcaaaag agtataatat ttttttccat cggacatcaa
    14881 cgcaggctgt tctttggaag caaacttata atggtacttg gcaaaaagac gtcttaaccc
    14941 aacatatgaa actgctgaaa actcggtacc tttatcgtct ttaagatcct gctcggcaac
    15001 cacattcatg gaaggaatat atacccacat catcttgcca tcggatacca cctgttgatt
    15061 acgaggtgcg ttaaacatta ttctaaggta atttggttga agcgactgaa taacgccttt
    15121 ttgtgtaact gaacccgacc cctttaaagt gaccatggta aaatcagctt gatatccttc
    15181 gactttagaa aaacggtcgc ggacattacg aacaacatcg ctgaccgtaa caaattcaaa
    15241 tttctgagca ttaacgctaa ttcctgtaaa aaacacaacc gctattgcgg cacaccatat
    15301 atattttctc ataccagatc ccgtctacct caattagaac ttgcttaaaa gcgagacata
    15361 cagggataca cttataaaac agcgtaaaaa agtcaatagt aaaacctgac ccgcatactc
    15421 atcttttgac agaatagaat agttgacttg tattattttg catatatagt tatttgattt
    15481 gcagaccgcc aacgagctcg atagaataat taatatcgat tacccctgct ttgaatatcc
    15541 ccgttaaaaa ctttaaactc gcttaataaa tgcaaggaac ctctattgat taacttatca
    15601 actcggaact tgcgaaaaat tccaataaaa taggtaatta aacaataata ccctccggaa
    15661 aaatgtaatt tttcgaagtt ccctgttatc aatttcactt attaatgaaa aacatatctt
    15721 gcaaaaaaac aggattatca aaaaaaatta caaaaaacca tttttttaca ttagaccgac
    15781 attctgttac aaaaaaaata ttcttcgcca attttagctt tacaaattat atttttaaaa
    15841 cttaattgtt acatcttcct ttctgaaatt ttctctacag ggttattctt atgcaaacaa
    15901 aaatagtcac tcgtctctca atgcctatat gcgatatcga agaaatggct tttgacgaag
    15961 agatagccct ctttgcggag aaacacaacc ttcttgaatc tgaaataatt tacttttcag
    16021 ccggcgagtc taaaattaag gaagttctga taaaacacga accatcactt tcagtttaaa
    16081 tatcacaaaa acagtttaat aataggggct taattttttc aaaaacttta aagccctact
    16141 tttttcagtt tggtacttgc tactttattt gtaatttcca aaacagcata ttgacctgct
    16201 ccgcttactc caggattaac aaacatcctg ttttcatgtt caattactac aggctcgtga
    16261 gtatgcccga acaacacaag agaggcatca agttggcgtg ccattaaaat cagttgcccc
    16321 atggtatgct ttacaccata caaatgccca tgggtcactg ctgtcatgac tccaaaaata
    16381 ttggttatta caagctcttc attatacatg gggctaaatc tgtcaatatt tcctggaaca
    16441 gctacaacag taaccgattc tctcaaaggt aaaaaagcaa tatcttttat tccatcaccg
    16501 caataaacca gaacttcaaa atctggttca gcatcaataa tagcttcaag tgccctgcaa
    16561 ttaccatgag aatccgaaac aaccaatacc ttgcccatca actacctgcc ataaccaaaa
    16621 atatagaaac aacagtcacc attgcccttt atttatcaat aaaaaacaga cttaaactcc
    16681 taaaccttct cgaaggtttt tattacaaaa aaagtacttg cccctaatcc tcgcaatata
    16741 taatcagcac aatagaggtt ttttatgcaa aacttaacta tgttttctta agcaaactac
    16801 caattactct gcttatatac aataaaatat caaggatacg gtaacacaat acgttctatg
    16861 aaaaaaaata atattcgcaa cttctcaatc atcgcacata ttgatcatgg aaagtccact
    16921 cttgcagaca ggataattga ctcgtgtact ccaataaaat cacgcattaa agttgatcaa
    16981 atgctagata caatggatct tgaaaaagag cgcggaataa ccattaaatc aaatgctata
    17041 tctcttgtat ataaggcaat aaacggagaa gattacgttt ttaacctgat agatacaccg
    17101 ggtcatgttg attttacata cgaagtatca cgatctttgg ccgcatgtga cggggtactt
    17161 cttgttgttg atgcgtcgca gggagtcgaa gctcaaacaa tagccaacat gtatatggct
    17221 cttgaaaaca atctcgaaat aatgccagta attaataaaa ttgatatgcc cgccgcagat
    17281 attgatcgca caaaagagtc gattgaaaaa acactgggac ttgaccctga agatgcaata
    17341 gctgtttctg ccaaagaggg tataaacatt gatatgcttc ttgaagcaat agtagaaaaa
    17401 attccggctc cgcaaggaga tgagaacaaa ccactacaag cattgatttt tgacagttac
    17461 tttgaccagt atgttggagc agtcgccaaa atcagggttt tcgaagggaa aatctcaaca
    17521 aatgatgaca taattttcat gtctaatgat aaaaagttta cggttaacga aataggagtt
    17581 tttggtttta aacccgaaaa acgccccagc ctgaaagcag gagacgtagg ataccttata
    17641 gcaaatatta agtccgtaac cgacactcaa atcggagaca cagttactca cgtctctaac
    17701 cgttgtgaga aaccactcag cggtttcaaa gaagcgaagt caatggtctt tgctggcctg
    17761 tatccaatgt tcagtgatga ttatgagaac cttcgtgaag cacttcaaaa actgcaactt
    17821 aacgatgcat ctcttctgtt tgaacccgat aactcttacg ctcttggctt tggatttaga
    17881 tgtggttttt tagggctact ccatatggaa ataattcagg agaggcttga gagagagttt
    17941 gatttatcgc ttgttacgac agcaccaagt gttgaatatc acgtaaagac aatacagggc
    18001 gaagagatga ttatagataa tcctgtaaaa tttcccgagc ctggaagcat agaatacata
    18061 aaagagccct atgttcgaac aacgatcatt accccaaaag aatttattgg taaccttatg
    18121 aatctttcca ttgattgccg tggaatacat aaggagatga aatatattga tgagaacagg
    18181 gtcgaaataa taaacgatat gcctttagca gaaattgttt ttaattttta cgacaaactt
    18241 aaatcgttat ccaaagggta tgcttcattt gattatgaac taatagatta tcgtgaatcc
    18301 aaaatggtca aagccgacat tctggtaaac ggtgaatctg ttgacgccct ctcgtttatt
    18361 gtgcataccg acaaagctta tcaacgagga aaggatatta tagaaaaact taaagatatt
    18421 attccacggc atcagtttaa aatacctatt caggcagcaa ttggcgctaa aataatcgca
    18481 cgcgaaacca taaatgcttt acgcaaaaac gtaactgcaa aatgttatgg tggtgatatt
    18541 tcacgaaaaa gaaaacttct tgaaaaacaa aaagagggga aaaaacgaat gaaactaatt
    18601 ggttcggtag agatccctca ggaagctttt ttatcaatat taagaattga ctagttggat
    18661 aattttgtat ggatattaaa aaccgtcttc ttttgagaag acggtttttt tatagataat
    18721 caatttttaa atcacatatt ttaaatagca gtaagaaccc ttacccattt atttttagaa
    18781 aaaatctatc tggcaataag cgcttctttt tttctgaaaa tatgatttac acgagtctca
    18841 tataatttat agacatcatc agaatctgtt ctttttaagt aatagtattt ttggtcatct
    18901 ttttttacaa tattaatagt cactgttttc ccatttataa ctacctccat aacacctaga
    18961 ggatcggaaa catcttcaat agcctcctca ctaagttgat cggctataaa tggattaaca
    19021 gcgttcatca ttgcaatggc actgttcata ttaacttttt cggaaccagt tttaaatttc
    19081 catctgcgct cgttctgcac agtaccatcc ggtgcttcaa cctcttcatc gtaaggcaaa
    19141 agcgtaaaag aggaaccttc gtatgacaca ctaaaagatt caatatctcg gtatgaaaat
    19201 ctggcaatct ccttacttct catttcgaaa acacttttac gtaaatcacg aggtgtaatt
    19261 gttccggcaa gatatatgga agaatcgtcc acgaatttaa cataagattg attcataccg
    19321 gtactctgct taccgaccat aatctcgaaa agcttattgt tcgccgactc aatagtaacc
    19381 cttattgcct gttcttcgtc caattcataa cgatacgaag catcatcaga cgaggagacc
    19441 ctgtcgaaga tttttaaagt ttttaatttt gaaacaatgg tctctgcctc tgtttcagag
    19501 gcaaccatat cggtattatt gatataccat ttatcgtctt tcttttcgag ttttaataca
    19561 tcacctccag gccgataaat agtaacaatt ttgggatcct cttcccattt tttaaattct
    19621 ggaagagata cgcccgagaa aaaaccttta ccaaaaatca gcaagagcaa aacaactatt
    19681 gttagaatac tgtataattt tttcttgttc atcacttaac ctctttcgca tatcgccgtt
    19741 cgattttttt tcgtctcatc attctaagcc tgataataac gagaaccata ataacagaaa
    19801 gcagcggaag ggcggctata ttgattattt taaaaacatt tttactggtt tcagatactt
    19861 ccttaagatt gctttcaaga cctttgcttc gcatctgcgg tacatcatcg aaaccgctca
    19921 tataatctac aagattatgt aaaaacagcg cattattata aacaaacatc tgacgtggat
    19981 cgttgttatt aatgttctga aggtttcctg aagttatttc cgaagtagag acaacagcaa
    20041 ttttggaatt actgatcgat tctttcagat gtttctcaac catcaacgaa gatgactctt
    20101 tcttttcacc atcatcgggt ttgccgtttg caaaatgaga ctcaaactta ccatcaagaa
    20161 ttacagcgag tggataagac tccatagcat ccttttcagg aatttccaga ccatatggtg
    20221 aaagatttat ctctcccgtc atttgccatg actctggtga agacgagacc agtacctcgg
    20281 acttgatatt ttttgactca agcttttgtt tatcaagttc aattgaagag gcttttacca
    20341 taagcatgta atttaaatag cttgtagaag gattatcttt attcaggttt tccctaaata
    20401 tttcaggagc gaaatagagg ttaaccggat ccatacctgg ctgctgcatt acaaacgact
    20461 gcatatccat tacataatct tggtttacct taacaccata agtctcaagc agtcccggta
    20521 tcccattctc gataggtcta aattgaggtg gttgctgcat ctgttgctgt tgtctgtttt
    20581 gaggtatttc gtaaaacata tctgtaaaga agagaacgtt tccacctcgc attatataat
    20641 tatcaattgc gtacagttca gcaggcgcaa gcggcaaaac aggaccatta ataatcactg
    20701 ttgatatggt ttcgggaatc gctccctgag ccaaatcaat catctgaatt tcatacatat
    20761 ccgatagaag gttcaaaagt gcaccaccag cctgctcatc ctgaacatct ttggtagcac
    20821 ccgttacaac gtaaccaata accatattac ttgaaatcag agaatcaatt gtcgacttta
    20881 cattttcatc caactcttca acccctgaga gcatcatacc aaacggagtc tgggcaacct
    20941 ggagatttat agccgatttt ttttctccat attctagcgt aacagttatc accccttgac
    21001 cggcggccaa tcttctcccg tcaggaagac gggtatcttc ccaagaaagc ggagttgcac
    21061 cgaattttga aacaagcatt tcaatcgtct gatcatctac agatgatact gacgagaact
    21121 cgacttttcc ggagttttct tttgaggctg aatcgacaac ctctttgaca gcagtttcaa
    21181 gtttgtttaa accattaata ttaaacatct ccaatttatc tgaaacataa agtgtaacct
    21241 taaccgattc acttcctgaa agcacatcga cttttccacg cattttggtg attttggttg
    21301 ttatcatata ctcaagacct ttaagacgag caacttgcgg tatcttgtcg ataatatcac
    21361 catgaataaa gaccatcccc atatacgcag atttagcctt gtattcgtct ccgctaagct
    21421 gacgaatctc tatcggctgt attccatacg atctcgcgat gttctttcct tcatcttttg
    21481 acatatcgac aaattcaaaa gtaaaattac catcagccgc gcttttatac tcttcaagaa
    21541 tgtctttcaa gtataaccgc acattactat atggagccgg aagatcttcc gaaaagaaaa
    21601 ctcttacgga taatggctct gtaatatttt taactgacct tttactgaca tccgaaagag
    21661 aatatactcc cccatcggta agatcgaaac gaagattgta tgatgaagca aatacattca
    21721 aaagtataag aattataaaa taaaatacgt aattatacgc gtctgtgaca aagaaatttt
    21781 ttacagacac caacatatca cgcatggcgt ttgttatcga ttctcgtact gccatattat
    21841 ctcctctcct ctattgatcg tactgaaagt ataatgaaaa agaggctcac cgttattaag
    21901 taagttatgt tgcgaaattc cagtacccct tttgcaagtc cctgaaaatg atacgaggca
    21961 cttaaatagt ttaagaatgc gaatatcttt cctggaataa attgtataaa ataatcaatc
    22021 atggtaaaga aaacacagaa agctgtagaa acaagccacg ctataatctg attctttgtt
    22081 agcgacgaag cgaaaatacc cactgcaaca taagatgctg caagaaaaat tgttcctata
    22141 taaccagcaa caatcatacc ataatccggc acaccggcaa ctccaagcga caacacataa
    22201 aaaagagttg gagccatcat aatcatcaca atagcgacag aagcaaaaaa cttacctaaa
    22261 acaagattag atgccgaaac aggcattgta ataatcatct ccaaagttcc actgcttcgt
    22321 tcttcagcaa tttgccgcat tgtgatagcc ggaataaaaa acatgaagaa aaaaggaagc
    22381 acgttaaaaa attgacgcat atccgcacga ccaataatat aaaaatcctt aaaatagaat
    22441 acacctgtca aaagtaaaaa cacagtcatt attatgtaca aaatcggact tcctacataa
    22501 ctcctaagct ctttaccaaa aagagctctt gtctggcgga agctatactt aaagtttatc
    22561 atattgttat tcattttcac cccccagagt cagatctcta aaaacctgtt caagactgtt
//
LOCUS       DAOTGZ010000016        22314 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_3064
            NODE_2750_length_22314_cov_5.074217, whole genome shotgun sequence.
ACCESSION   DAOTGZ010000016 DAOTGZ010000000
VERSION     DAOTGZ010000016.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868088
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 22314)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 22314)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:22:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,785
            CDSs (total)                      :: 2,751
            Genes (coding)                    :: 2,732
            CDSs (with protein)               :: 2,732
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 12 of 19
            Pseudo Genes (internal stop)      :: 5 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22314
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2750_length_22314_cov_5.074217"
                     /isolate="AMR_MDS_3064"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..792
                     /locus_tag="P5123_02300"
     CDS             <1..792
                     /locus_tag="P5123_02300"
                     /inference="COORDINATES: protein motif:HMM:NF015113.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="His/Gly/Thr/Pro-type tRNA ligase C-terminal
                     domain-containing protein"
                     /protein_id="HRX15124.1"
                     /translation="KSFLGAAELEQADDKTVESVTGSAVGFAGPVNCDAKRIVFDYGV
                     AQVQGAITGANKTDYHFAGVRPGRDFVISEEADIVEAIDGDLCPECGKPMITRKGIEV
                     GHIFKLGKKYTESMKVNVLDNNGKAVVPLMGCYGIGVTRTLAAVIEQHHDEKGLIWPK
                     AVAPFDVHLVGIGKSDDEKTAIDEVYTTLLSDGLEVLYDDRKLSPGVKFADADLLGLP
                     VRITCGKSYFQDGELEIKIRKTGEQLTVKPDDLLKKVREIFAGLD"
     gene            919..991
                     /locus_tag="P5123_02305"
     tRNA            919..991
                     /locus_tag="P5123_02305"
                     /product="tRNA-Ala"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:952..954,aa:Ala,seq:ggc)
     gene            complement(1070..3694)
                     /gene="adhE"
                     /locus_tag="P5123_02310"
                     /gene_synonym="adhC"
     CDS             complement(1070..3694)
                     /gene="adhE"
                     /locus_tag="P5123_02310"
                     /gene_synonym="adhC"
                     /EC_number="1.1.1.1"
                     /EC_number="1.2.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004618488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004022 - alcohol dehydrogenase (NAD+)
                     activity [Evidence IEA];
                     GO_function: GO:0016620 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, NAD or NADP as
                     acceptor [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006066 - alcohol metabolic process
                     [Evidence IEA];
                     GO_process: GO:0015976 - carbon utilization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional acetaldehyde-CoA/alcohol
                     dehydrogenase"
                     /protein_id="HRX15125.1"
                     /translation="MKTLTEKKIMTEINSDTPSVNKIIKNVKKAQQQYSKFTQQQVDH
                     IFYSAAMAANAKRLELAKMAVAETGMGIVEDKVIKNHFASEYIYNKYKNLKTCGIIED
                     DPIQGITKVAEPLGIIAGIIPTTNPTSTAIYKALMALKTRNGIIFSPHPRAKNCTIEA
                     AKIVLNAAVKAGAPDGIIGWIEDPTVEMSNQLMTDSEISTILATGGPGMVKAAYSSGK
                     PAIGVGSGNTPVIIDETADIPMAVASILISKTFDNGVICASEQAIVVHEKIYDDFKKE
                     LTKQGAYVLNKKERNLVASTIIIDGRLNAKIVGQSAYTIAEIAGIQVDKKTKVLIGEA
                     EAIDHKEPFAYEKLSPVLGLYKASDFGDALEKADALVHFGGLGHTSVLYVHSNETNKI
                     ETFGQRMKTGRTLINMPSSQGAIGDLYNFALDPSLTLGCGSWGGNSISQNVTPEHLVN
                     IKSVARRKENMLWFRLPEKVYFKAGSLNYALDDLADKKRAFIVTDDFLYKNGYLNGLT
                     EKLNTMGIAYSIFSDVKPDPTVETALEGAKKMGMFEPDVIIAMGGGSPMDAAKIMWLL
                     YEHPETDFESMSMRFMDIRKRVFTFPKMGQKAIFVAIPTTSGTGSEVTPFAVITDEKR
                     GVKYPIADYALTPNMAIVDANLVRDMPKGLTAFSGIDALTHALEAIVSVLATEYTTPI
                     AMEAARLIFEYLPKAYVNGNNMKAREKVHHAANMAGMAFSNAFLGVCHSMAHKLGSFH
                     NLPHGLANALLINEVIRYNSVLVPNKQGTFPQYEVHEAAERYARLSDYLGLGGSTIED
                     KVQKIIDKIDELKKQIKIPDCINDAGVEENEFMKTLDDMAEKAYDDQCTGANPRYPLI
                     EEIKDMYKTAFYGKSQEK"
     gene            complement(3706..4341)
                     /locus_tag="P5123_02315"
     CDS             complement(3706..4341)
                     /locus_tag="P5123_02315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013254280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="redox-sensing transcriptional repressor Rex"
                     /protein_id="HRX15126.1"
                     /translation="MKPVPYPTIQRFPLYLEVIKQLQIAGIKTISATDIAQNLGFTNI
                     QVRKDIGYTGIIGKPKIGYNIKELEKVITSFLGWQNTNDAILIGVGALGSALLGYKGF
                     EDYGLNIISGFDVNKKIVGKKIHGKTIMHIDDLKSQVKRLKVSIGILTVNQSSAQQVT
                     DLMVKAGIRGIWNFSSENLKVPNDIVVQRENLSASLALLSVKLSHKFKTKQ"
     gene            complement(4676..5308)
                     /gene="prfH"
                     /locus_tag="P5123_02320"
     CDS             complement(4676..5308)
                     /gene="prfH"
                     /locus_tag="P5123_02320"
                     /inference="COORDINATES: protein motif:HMM:TIGR03072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003747 - translation release factor
                     activity [Evidence IEA];
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor H"
                     /protein_id="HRX15127.1"
                     /translation="MKLQISSGRGPVECERGVYLFYEKYLSPLVAKADIKSDIISSIK
                     GVEVNCFKSLTIDIQVNIESDLGKKLESLCGTILWICQSPYRKGHRRKNWFINISFLD
                     EKEGLTFHENELVYEISKSSGPGGQNRNKVETAVKVIHKPSGITATATEERSQKLNKE
                     MALKRLHKVFQQKIETTEKQEKNQQWSHHNYLIRGNPTLVFKGEKFELSR"
     gene            complement(5317..6366)
                     /locus_tag="P5123_02325"
     CDS             complement(5317..6366)
                     /locus_tag="P5123_02325"
                     /inference="COORDINATES: protein motif:HMM:TIGR03073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA ligase RtcB family protein"
                     /protein_id="HRX15128.1"
                     /translation="MNSKISIIASSTSWIESSAIDQMEALARLKGVEYIIGLPDLHPG
                     RGIPIGAVCITKDIIYPHIIGNDIGCGMSLFKTNIRSNKFSTNKIADKLEKNIGNENS
                     GSIGGGNHFVEFLVVESIDDQMVEDTLIDTKSIYLLVHSGSRGYGNMIYNHYLREHNA
                     QHGFETESKLYKNYLKDHDNACEKASENRNNIAIDICHNSGINLEEKPVIESIHNYVE
                     MLTIDNREYIIHRKGASSALCNYAVIPGSRGDFSHIVKPVNAGVKSGFSLPHGAGRKW
                     NRNQCKDRLLNKYTKESIKRTKLGSRVVSANSDILFEEAPEAYKKIAEVIGDMEKAEL
                     LQKYVTLRPLITYKA"
     gene            complement(6701..9265)
                     /locus_tag="P5123_02330"
     CDS             complement(6701..9265)
                     /locus_tag="P5123_02330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020585504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HRX15129.1"
                     /translation="MNRIGKNILLVEDEMLTATLEKSELEKYGYSVIHVATAIEAIKT
                     ALETDKQLDLILMDIDLGHGMDGTEAAEKIVKDKEIPIVFLSSHTEPSIVEKTEKITS
                     YGYVVKNSGIVVLDASIKMAFKLFEAKCRLQVEIENHKRIEKELQESEERFKALHNAS
                     FGGIVIHDKGVIIDCNNGLSLMTGYTIDELIGMDGLLLIAEEDRDYVLNKIVTGYEQA
                     YEAKGCRKNGEIFPMRLEARNVPYKGKMVRTVEFRDLTEVKNTEEALKLNELKWKSYI
                     EYAPYAIFVSDKEGRYIDVNPMASKITGYSREELLCMTILDIVPQNRRKEGYEHFKNV
                     IKNGIYEKEVSFLAKNGEHRYWNVAAQKISDNIYLAYCNDTTENRKSHKQLRLSEERY
                     RNLFTTMAQGVVYQDVNGAIISANPAAEEILGITFDKMRGITSIDPRWQAVDENMNKL
                     PGEEHPSMIALKTGKVIKNFIQGIYNPKRNDYVWIIINSVPLFKEGEDKPYQVYSLFL
                     DITERKHYEEALRKSEAYTKAIMDNLPIGIAINTVTPIVKFTYMNDNFPVFYHTTRKK
                     LFENNSFWETVYKDKNFRDKLQKQVEMDCASGDPTRMQWDDIPFISDGKTYYICARNI
                     PLPNSNEMISTVWDATNRKETELELTRQLNEKEIILKEVNHRIKNNIHSIEAILSLQL
                     ADTKSLESKEIVADVLRRIQSMRIIYDKLLLSRDYKNTSVKAYLEDLLSAIIQVFSDK
                     KNIKITTSIFDLNMNAKILFPLGSIVNELVTNSIKHSFETKNKGTIQVILIKKETLIE
                     LTVINDGDRLPKDFDPERNSGFGLMLVKMLSQQLQGSFTIDNNDTGTISIVTFPCE"
     gene            complement(9427..10212)
                     /locus_tag="P5123_02335"
     CDS             complement(9427..10212)
                     /locus_tag="P5123_02335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004329076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005575 - cellular_component [Evidence
                     IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR02757 family protein"
                     /protein_id="HRX15130.1"
                     /translation="MDFKSKQKIAQLLEAEYVNNNTPEFIECDPIQIPHNFKKQEDIE
                     ISAFLTAQIAWGKRSMIIRNGYNLMNIMDNSPYDFVLNASNNELKKIDGFVHRTFNAD
                     DCRTEILALRRFYMKYSNLGAYFESVFNTEGSIRNTLIQFRDDFLNSNHPPRFTRHIS
                     DVRKKSAAKRLNLLLMWMCRNDGRGVHFGLWNIPTSALMIPLDVHCGNAARSMGLLNR
                     KQNDWLAVEELTLKLREFDPHDPVKYDFSLFGLSIQGKLKTCD"
     gene            10721..11302
                     /gene="ruvA"
                     /locus_tag="P5123_02340"
     CDS             10721..11302
                     /gene="ruvA"
                     /locus_tag="P5123_02340"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: protein motif:HMM:TIGR00084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009379 - Holliday junction helicase
                     complex [Evidence IEA];
                     GO_function: GO:0009378 - four-way junction helicase
                     activity [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration protein RuvA"
                     /protein_id="HRX15131.1"
                     /translation="MIGSLKGSIQKLKTSSLIIDVSGVGYFVSISLNTYYNLADKQTV
                     FLYIYTYIKEDKFDLYGFLTEEEKDIFKVLISVTGVGPSLALAILSGMSFTELLNVIR
                     NRDVAMLTKIPGIGKTKAEKILLDLQSKAKKLNLQSSETISGGNPFLEDAVDALVALG
                     FEERKSRIAVADCIGSNNPEDLQEIIKGSLKLL"
     gene            11349..11879
                     /locus_tag="P5123_02345"
     CDS             11349..11879
                     /locus_tag="P5123_02345"
                     /inference="COORDINATES: protein motif:HMM:NF015513.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HRX15132.1"
                     /translation="MDQKKKKKESVNTIKLIILGMLMERNLYGYEINKIIRERFQGYV
                     DIKFGSIYYAIKEAIKKGYVKKYETIKDTPTPPKDIFKIEQAGRKQFKILLRRYFSEN
                     FIHFDVDIALMFLNSLTDDQKETFIEERKELLKERLADIKKAINGDDGSDKDYMHIYT
                     YLENHLKAENNWVKSL"
     gene            complement(11981..13042)
                     /locus_tag="P5123_02350"
     CDS             complement(11981..13042)
                     /locus_tag="P5123_02350"
                     /inference="COORDINATES: protein motif:HMM:NF012257.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030551 - cyclic nucleotide binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic nucleotide-binding domain-containing
                     protein"
                     /protein_id="HRX15133.1"
                     /translation="MNYKLLYEKGHDPDTVYIMQSGEVIFFATNDDRYRITGQNLIIG
                     TSEIILSNLLGIPTKRVETAVVPQNTDVKYVKIDTFLEQFKQEGYLINIAKVTAQKLR
                     LTNSIIKKNQDILLSGKKTLKQLFTQYYSIVSILRDEYNKRKLPWLKDTINKYTSSIQ
                     FLKAESYAKADIPVKIYETEALSEKTIDLPPDHILCKEGDNDNEMFILQSGTLEVTVN
                     SNRVALINEVGSTIGEISLLLGEKRSATIKTLTPVRVTKLNLNDLKTIAKTDIETILQ
                     IVYSLTKKFYNNISLIRDINASVLNKKLEEEEGKISERKEISSISHDLEKLKFHVDEL
                     LYSHNADYIREKLDRFLKI"
     gene            complement(13039..13395)
                     /locus_tag="P5123_02355"
     CDS             complement(13039..13395)
                     /locus_tag="P5123_02355"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HRX15134.1"
                     /translation="MANIMIVDDSKFMRSIIKETLIENNHTIVAETDNGVDAITLYND
                     LHPDIVTMDITIHGKDGIQTIEDIKKDNPDAKIIVVSALSKKTLQNSSRDLSVTAYVT
                     KPFKKEDLQNAVNSLI"
     gene            complement(13724..14314)
                     /locus_tag="P5123_02360"
     CDS             complement(13724..14314)
                     /locus_tag="P5123_02360"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF014591.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Maf family protein"
                     /protein_id="HRX15135.1"
                     /translation="MIIILGSSSPRRHYFFSLLFDEFQQITPEIDEAPFSNETALDYA
                     LRISSEKASWIIKEKVTSDNFFVVTADTVVSLDNKILGKPKNRAEAENMLTRLSGQRH
                     EVITSISMAHPAKESLKIVSDYDVTEVIFKKLSNSEISRYLNSIDWHDKAGSYAIQEH
                     PELIISSVNGSISNVVGFPVRVFLKYCALKTPEIFK"
     gene            complement(14311..16356)
                     /locus_tag="P5123_02365"
     CDS             complement(14311..16356)
                     /locus_tag="P5123_02365"
                     /EC_number="2.7.6.5"
                     /inference="COORDINATES: protein motif:HMM:TIGR00691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0015970 - guanosine tetraphosphate
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional (p)ppGpp
                     synthetase/guanosine-3',5'-bis(diphosphate)
                     3'-pyrophosphohydrolase"
                     /protein_id="HRX15136.1"
                     /translation="MSEYNYNDIFDLFEDLLQIISVKHPDIDVTLIKKAFAVAESAHR
                     DQKRLSGEPYIIHPINVAIILAKHSLDSSSIIAALLHDVVEDTVTGLDSIADTFGSEI
                     ALLVDGVTKISSLKNRTKSHEQAQTLRKMLLATIDDPRVIIIKLADKTHNMRTLEFQP
                     KPKQLKIAQEVMDIYAPLAGRLGMSKIRSELEDLAFYILHRDEFLDITAKLSKQEEEL
                     DEYLNEIHSTLHSNISKMNIKADISGRIKHLYSIYKKMVDQQKPFEEIFDVRAIRIIT
                     EEVKDCYAILGIVHTIWPPVPSRFKDYIAVPKSNMYQSLHTTVTGPGKHFLEIQIRTK
                     EMDITAEMGIAAHWMYKENGKKEKSKMSDLSLLKSINKWRSELNDTREFMKVLKMDLY
                     NDEIFVFTPKGKIIRLAKGATPIDFAYAIHSEVGHHCSGAKINHKIKPLRTKLSNGDI
                     VEITTNPAKHPSENWLKIVRSSNARYKIRSWLKKQQPTDSDEASSKSKKDTKSEPDKI
                     ASFTIDMAEMPKISKSPDNLKSGILVEDNNNVPIRLAQCCQPIPGDPVIGFISKDKRI
                     SVHKKGCPSLKRLNIEPERFISIAWSNSHHVHPVKIAIEAIDRPNLLADITTEISQSR
                     INIMKLEAQESKAGFAVIRLIVEVKSVDHLHFVIDRLKSVKDVVEVYKLKEKVIKRK"
     gene            complement(16663..17241)
                     /locus_tag="P5123_02370"
                     /pseudo
     CDS             complement(16663..17241)
                     /locus_tag="P5123_02370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004512841.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(17238..18434)
                     /gene="rlmD"
                     /locus_tag="P5123_02375"
     CDS             complement(17238..18434)
                     /gene="rlmD"
                     /locus_tag="P5123_02375"
                     /EC_number="2.1.1.190"
                     /inference="COORDINATES: protein motif:HMM:TIGR00479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008649 - rRNA methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (uracil(1939)-C(5))-methyltransferase
                     RlmD"
                     /protein_id="HRX15137.1"
                     /translation="MGKRTRKNKELILDIAEKYRGTGETLCSHFGECGGCLFQDISYE
                     NQLEIKAQYLNQVCADVLPRIENIHATEPFGYRNRMDYVAAFGCRGFRKRGSFKQVVD
                     ITECPLLQNNSEEVWKRIHNSIHEIEDYDYLSHHGFLRYTVLRQGFFSGETMLNFVVS
                     EKKDLEIFEKILNSVDSLVNSSSILLNDSLTDLSFGPEIHTIKGGSITESFDLENSPH
                     NNNKSIKFTIHPNTFFQSNSKVAVNMYRKIRELVSGRVLDLYCGVGSISLFCADQCES
                     ITGVDIVEDSINSAKQNMQLNNIENASFVCADARPFIKENSKSFDTLILDPPRAGINP
                     RMYKYLHSLSPSRIIYMSCNPVTFKSDYENLKEQYSLESFEVFDMFPQTPHMESLALL
                     VKRGNI"
     gene            18777..19874
                     /gene="ispG"
                     /locus_tag="P5123_02380"
                     /gene_synonym="gcpE"
     CDS             18777..19874
                     /gene="ispG"
                     /locus_tag="P5123_02380"
                     /gene_synonym="gcpE"
                     /EC_number="1.17.7.1"
                     /inference="COORDINATES: protein motif:HMM:NF001540.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046429 - 4-hydroxy-3-methylbut-2-en-1-yl
                     diphosphate synthase activity [Evidence IEA];
                     GO_process: GO:0044237 - cellular metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin-dependent
                     (E)-4-hydroxy-3-methylbut-2-enyl-diphosphate synthase"
                     /protein_id="HRX15138.1"
                     /translation="MKERTRKASIGSVQIGGGLPVLVQSMCNIPLSETDSVINQIGQL
                     HDRGARLVRFSVRNMNEAAYIRKVINSSEIPVCADIHFDYKLAIAAIEAGVAKIRLNP
                     GNIKSEDGVRRVVEAAKSNGTAIRIGVNGGSVNRTGFSGSEERILVDHAFEHLRILEN
                     NGFYNTVISIKSSDVLSTLKANRLLATECDYPIHIGLTEAGYGNNALIKSSVTIGALL
                     LDGIGDTVRVSMTGDPLQEIDAAYEILKAVHRLDYGINIISCPTCGRTDRLIDLEALA
                     RDIDKAVNAKYLKLVRDQKKILNIAVMGCEVNGPGEASEADIGVAGCGRGQLALFLRG
                     KIIKKVNQDRVVDQILEYTGKIVAGISLPAE"
     gene            complement(19903..20742)
                     /locus_tag="P5123_02385"
     CDS             complement(19903..20742)
                     /locus_tag="P5123_02385"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HRX15139.1"
                     /translation="MKKNKQNISLLKVENIKLKFGSRMILSDVNFSVEPGTITVITGK
                     SGCGKTTLLGIISGLLKPNDGKVYFKGKNILRWGDIRRSFYRSKSIGFIFQFFNLLND
                     YSAFENILLPTMFNPFARNASKKAKELIEYLNLKAISSNHPETLSGGERQRIAIARAV
                     INNPDIILADEPTGNLDDESAEDINNLFIKLAKENNKAFVIVTHDHRIVENSDFHYHL
                     SDTALVLQAKTKNRSKKPAAKKSSSIAKIKPAGKTSQNKSDSSSRKNTKKTAKKTSAK
                     KTK"
     gene            complement(20752..22083)
                     /locus_tag="P5123_02390"
     CDS             complement(20752..22083)
                     /locus_tag="P5123_02390"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRX15140.1"
                     /translation="MVHLLRSIYYGLRSSFKSIITSFFSSFGILFIITFLIIYLSFRT
                     SISDFIDENLLGSLEIDEIEILPINEKADNVYAASSRQGAKLTIDQVKAVREMDDFSE
                     VFTLIMLDYRVRLRGEMFGNRSTMILPLFAIEPEFFKNKIPKWDQFYYKPGRPLKMIA
                     PKFTLVMLNNYLYFNGLPQFKIEDLKGFPMEIQIETLKSPRPFPGAKIEFKEDKGKTH
                     TDLDVSVFSFTNVTERIGAIVPMDFITYFSKRAEVSSGRWKRGYHYLQFYAKVKDIKK
                     LPETVDKLEKLGLQVKSDRGIADKANQVMKIIDAFSLTIVGILILLTIVSIFNANLNV
                     VYHMSHKFSLTRILGVTKIRIIITFIVEAAIVGAIYGIIGYYAGNYLFSYAAESISSF
                     LPDIAGIEFVAQRNKEVFMIAMGLSALVSASSALLPAIFASNINLFKAVRR"
BASE COUNT         6590 a         3874 c         4145 g         7705 t
ORIGIN      
        1 aaaagctttt tgggcgcagc cgagcttgaa caggctgatg ataaaactgt agagtctgta
       61 acaggtagcg ctgtcggttt tgcggggcct gttaattgcg atgcgaaacg cattgtgttt
      121 gattacggtg ttgctcaggt tcaaggagcg attaccggtg cgaataaaac agattatcat
      181 tttgcgggag tacgcccagg gcgggatttt gtgattagtg aagaggcaga tattgtagaa
      241 gctatagatg gcgatctttg cccggaatgt ggtaagccca tgattacacg taaaggtatc
      301 gaggtcgggc acatatttaa acttggtaaa aaatatacag aatcaatgaa agttaacgtt
      361 cttgataata acggtaaggc tgttgtacct ttgatggggt gttacggaat cggagtgact
      421 cgaactttgg ccgcggtaat agaacaacat catgatgaga agggattaat atggccaaaa
      481 gccgttgctc cttttgatgt tcaccttgtg ggaatcggaa aaagtgatga cgaaaaaaca
      541 gcaattgatg aagtatatac gactctttta tctgatggtc tggaagtatt atatgacgac
      601 agaaaattaa gtccgggagt aaagttcgct gatgctgatc ttttgggctt gcctgtaaga
      661 ataacttgtg gaaaatcata ttttcaagat ggtgaacttg agataaaaat ccggaaaacc
      721 ggagagcagt tgacagtaaa accggatgat cttttaaaaa aagtcaggga aatatttgca
      781 gggcttgatt aaaaatttgc taataaccct cgtcggtctg gttgataata agcaatttta
      841 tgttttttta gtatccaatt gagatttaaa agctatatgc ttgactaaaa aatgtcaatt
      901 ttatagttgt taatttatgg ggatgtagct cagctgggag agcaccacaa tggcattgtg
      961 ggggtcgcgt gttcgatcca cgtcatctcc aaaaattaca atattacaaa aggggctctg
     1021 cgatttcgct gagccccttt tgatgtgaaa ttataattct tagtactgac tatttttctt
     1081 gagatttacc ataaaaagct gttttataca tatccttaat ttcttcgatt agtggataac
     1141 gcggattagc acctgtacac tgatcgtcat acgctttttc tgccatatcg tctaatgttt
     1201 tcatgaactc gttttcttct accccagcat cgtttatgca atcaggaatt ttaatctgtt
     1261 ttttgagttc gtcaatttta tctataatct tttgaacctt gtcttcgata gtacttcctc
     1321 caaggccaag gtaatctgaa agtcttgcat atctttctgc agcttcatga acctcgtact
     1381 gcggaaaggt gccctgtttg tttggcacta aaacactgtt ataacgaatt acttcgttta
     1441 tcaaaagtgc gttggctaga ccatgaggaa gattgtgaaa agagcccaat ttatgcgcca
     1501 tggagtgaca aacgccaaga aatgcgttag agaaagccat ccctgccata tttgcagcgt
     1561 ggtgaacctt ttcgcgagcc ttcatattat ttccattaac ataagctttt ggtaaatatt
     1621 caaaaataag ccgagctgct tccatcgcta ttggggttgt gtattctgtt gccagaacgc
     1681 tgactattgc ctcaagggca tgtgttagtg cgtctatgcc ggaaaaggcg gtaaggcctt
     1741 tgggcatatc tcttacaaga ttcgcatcaa ctattgccat attaggtgtc agggcataat
     1801 cggctattgg atacttaaca cctctctttt catcagtaat cacggcaaaa ggtgtgacct
     1861 ctgacccggt tccactcgtt gtgggtatgg cgacaaaaat agccttttga cccatttttg
     1921 ggaaagtaaa aactcttttt ctaatatcca taaaacgcat tgacatggat tcaaaatcag
     1981 tttcgggatg ctcatataaa agccacatta ttttcgctgc atccattggt gagccaccac
     2041 ccatggcaat aattacgtcc ggttcgaaca tgcccatctt ctttgcacct tctaaggccg
     2101 tttcgacagt tggatcgggt tttacatcag agaaaataga gtaggctatt cccatagtgt
     2161 ttagtttttc agttaaacca ttaaggtagc cgttcttata tagaaaatca tctgtaacaa
     2221 taaaagcacg tttcttgtcg gcaagatcgt ccagcgcgta atttaatgag ccggccttga
     2281 aataaacttt ttccggtaat cggaaccaga gcatgttttc ttttcgtcgg gcaacagatt
     2341 tgatgtttac cagatgctcc ggcgttacgt tttggctaat actgttgcca ccccagctac
     2401 cgcaaccaag ggttagggaa gggtcaagag caaaattgta taagtctcca atcgcgcctt
     2461 ggctagacgg catatttata agagtcctgc cggttttcat acgttgtcca aaagtttcaa
     2521 ttttatttgt ttcgttgcta tgaacataaa gaacgcttgt atgaccaaga ccaccaaagt
     2581 gaacaagtgc atctgctttt tccaaagcat caccaaagtc agatgccttg tataatccaa
     2641 gtacaggcga gagtttttca taagcaaatg gttctttatg gtcaatagcc tcagcttcgc
     2701 cgataagaac ttttgttttt ttatctactt gaataccagc aatttctgca atagtatatg
     2761 cgctctggcc aacaattttt gcgttaagac gaccatcaat aattattgta cttgcgacaa
     2821 gatttcgctc ttttttgttt aaaacgtagg ctccttgttt ggtgagctct ttcttgaaat
     2881 catcgtatat tttttcatgt acaacaatgg cttgctcgga agcacaaatt actccattat
     2941 caaatgtttt gctgataaga attgatgcga cagccattgg tatatcagct gtttcgtcaa
     3001 taattactgg agtgtttcca gaacctactc ctattgcggg tttcccagac gagtaggccg
     3061 ctttaaccat gcctggaccg ccagtggcaa gaatagtgct tatttcagag tcagtcatca
     3121 gttggttgga catttcgaca gttggatctt caatccagcc gattatacca tcaggagcac
     3181 ctgcttttac agcagcattt agcactattt ttgcagcttc aatcgtacag tttttagccc
     3241 tggggtgtgg tgagaaaatt atgccattac gagtttttag agccataaga gctttgtaaa
     3301 ttgcagtgga tgtcggattt gttgttggaa taattcctgc aattatgcct aagggttcag
     3361 ctacttttgt tatgccttgt atcggatcgt cttcaataat tccgcatgtc tttaagtttt
     3421 tatatttgtt gtaaatatat tctgaagcaa aatgattttt gatcaccttg tcttctacaa
     3481 tacccattcc ggtttcggcc acagccattt tggctagttc cagccttttt gcgtttgctg
     3541 ccatagctgc cgagtaaaaa atatggtcga cctgctgttg tgtaaatttt gagtattgtt
     3601 gttgagcttt ttttacgttt ttaatgattt tattgaccga tggcgtatca ctgtttattt
     3661 ctgtcatgat tttcttttcg gttaatgttt tcatatttgc ctccattact gtttggtttt
     3721 gaacttatga gaaagcttta cagagagtaa agccagactg gccgacagat tttcccgttg
     3781 aacaactata tcattaggaa ccttaaggtt ttcagatgag aaattccata ttccacgaat
     3841 accagctttt accattagat ctgttacctg ttgcgccgaa ctttgattta cagtcagaat
     3901 cccgatagat actttcagtc gtttgacctg cgatttaagg tcatcaatgt gcatgattgt
     3961 tttgccgtgg atttttttcc ctactatttt tttattaaca tcaaatcctg aaatgatatt
     4021 caaaccataa tcttcaaacc ccttgtatcc tagcagtgca gatcctaaag caccaactcc
     4081 gatgagaatg gcatcatttg tattttgcca tccaagaaaa gaggttatga ctttttccaa
     4141 ttctttaata ttatatccaa ttttaggttt gccgatgatt cccgtataac ctatatcttt
     4201 gcgcacttga atatttgtaa aaccaagatt ttgtgcaata tctgtggcag atatggtttt
     4261 aatacctgct atttgtagtt gctttataac ttcaagatac aaaggaaatc tttgtattgt
     4321 cggataaggt actggtttca ttgctgctcc tttaatcgat aaatatatat cgttataaat
     4381 atatcgatat atatttatct aaaagctgtc aatatatttg cacttttttg tgattaaaat
     4441 cacaaaaaag tgcaaaccgt ccagacggtg aataatctaa tgaggaagtt gtttaatctt
     4501 gatttatgcc tgttttgtta aaaatatttt ggataatatt tcttaaaatt cagaccgtat
     4561 atatgtagga agatatagag tagaactaac tatttttatg agattctaag agttattagt
     4621 ttgtcgaaaa aacaatgaag tgacttttaa agccacttca ttgttagact atttattacc
     4681 ttgataactc aaacttttca cctttaaaaa caagagttgg gttgcctctt attagataat
     4741 tgtgatgtga ccattgctga tttttttcct gtttttcagt agtttcaatt ttttgttgaa
     4801 aaactttatg aagacgtttg agcgccatct ctttgtttag tttttgagaa cgttcttctg
     4861 tagctgtagc agttatcccc gaaggtttat gaataacctt gactgcggtt tcgaccttat
     4921 tacggttttg accacccggc ccagaacttt tacttatctc ataaacaagc tcattttcgt
     4981 gaaaagtaag accttctttt tcgtccaaaa agctgatatt gataaaccag tttttacgcc
     5041 tatgaccttt tcggtaaggg ctttggcata tccaaaggat agttccacat aaactttcaa
     5101 gttttttacc taagtccgat tctatattta cttgaatatc aatcgttagt gatttaaaac
     5161 agttaacttc cactcccttg atagatgaaa taatatcaga cttgatgtcc gcctttgcaa
     5221 caagcggtga caaatacttt tcataaaaga gatatactcc acgctcacat tctacgggtc
     5281 ctcgaccaga actaatctgt aatttcataa cacctcctat gccttataag taatcagagg
     5341 ccgaagcgtt acgtacttct gcagcagctc tgctttttcc atatcaccga taacttcagc
     5401 tatctttttg taagcctcag gtgcttcttc aaatagaata tcactgttgg ctgatactac
     5461 acgactgcct agcttagttc gtttgatcga ctcttttgta tacttgttaa gtaacctgtc
     5521 tttgcactga tttcggttcc attttcgacc agctccatga ggaagagaaa aaccgctttt
     5581 tacgcctgca tttacgggtt ttacaatatg gctaaaatcc cctctgctgc ctggtatgac
     5641 tgcatagtta caaagtgctg atgatgctcc tttgcggtgt atgatgtact ccctattatc
     5701 gatagttagc atttctacat agttgtgaat gctttcaata accggttttt cttccaaatt
     5761 aatgccagaa ttatgacaga tatctatagc aatattgttt ctgttctctg aagctttttc
     5821 acaagcatta tcatgatcct taagataatt cttgtaaagt ttgctttctg tttcaaaacc
     5881 atgctgcgca ttatgttcac gaagatagtg attgtaaatc atattgccat acccacgaga
     5941 accgctgtgc acaagcagat agatactctt tgtatcaata agagtgtctt ctaccatttg
     6001 atcatcaata gattccacca cgagaaattc gacaaagtga ttacccccac cgattgatcc
     6061 tgaattttca ttgccaatat tcttttcgag tttatcagca atcttgtttg tagaaaattt
     6121 gttagacctt atgtttgttt taaaaagtga cattccacaa ccaatatcat ttcctataat
     6181 atgtggatag attatatctt tggtaatgca gactgctcct ataggaatac ctcgaccggg
     6241 gtgtagatcg ggtaacccga taatatattc tacacctttg agccgagcga gagcttccat
     6301 ttggtctatc gcacttgact ctatccacga tgtggaagag gcaatgatac ttattttact
     6361 gttcatttaa taatttccta ttatttgaga gaatatgaca tattaaaaga ttatgacata
     6421 actcaatttt gccattgtta atataacaag gcgcaagtaa aaacggtctg ttcaaataca
     6481 gacttgatta aaatttagat tgtaatattg aaaggggagt gcctgaggta agaaaccaaa
     6541 gagggtaaag tacaaatctt tattttctta tcttgcagga agccctttgt tattttgatt
     6601 gatcatatta gcctccgata atgatttaat tgttgaataa atatagagac agattttata
     6661 ttgtcaagat aaaatttcaa ataagttaat tccttcataa ttactcacat ggaaaagtga
     6721 cgatactaat agtaccagta tcattgttat caattgtaaa agatccttgc agttgttgtg
     6781 agagcatctt taccaacatc aaaccaaatc cgctgtttct ttccgggtca aaatcttttg
     6841 gaagtcggtc tccatcattt attactgtga gttctattaa cgtttctttt tttattaata
     6901 taacctgaat ggttccttta ttttttgttt caaaagaatg ctttatagaa ttagtaacaa
     6961 gttcattaac tatagaccct aaagggaaaa gtatttttgc gttcatgttt aaatcaaaaa
     7021 tacttgtggt aatttttata ttttttttgt cggaaaaaac ctgaataatt gcggatagta
     7081 gatcttcaag ataagctttg accgacgtgt ttttatagtc acgagatagt aatagcttat
     7141 cgtaaattat acgcatgctt tgaattcgtc ttagtacgtc agcaacgatt tcttttgatt
     7201 ccaaactttt tgtgtcggct agctgtaaag acaatatcgc ttcaattgaa tgtatattgt
     7261 tttttattcg atggtttacc tcctttagaa taatctcttt ttcattaagt tgtctggtca
     7321 attcaagttc cgtttctttt cggtttgttg cgtcccacac agtagatatc atttcatttg
     7381 aatttggcag ggggatgttt cgcgcgcaga tgtaataggt cttaccatcg ctgataaagg
     7441 ggatatcatc ccattgcata cgggttggat ctcctgatgc acagtccatc tctacctgtt
     7501 tttgcagctt atcacgaaaa tttttgtctt tgtaaacagt ttcccaaaag gagttgtttt
     7561 caaaaagttt tttacgtgtt gtatgataaa atacaggaaa attatcattc atatatgtaa
     7621 atttaactat aggtgtaact gtatttattg ctataccaat aggtaaatta tccataattg
     7681 cctttgtgta ggcctcgctt tttcttaatg cttcttcata atgcttacgt tcagtaatat
     7741 ctagaaaaag tgaatatact tgatagggtt tatcttctcc ctctttaaaa agtggaacag
     7801 aattgatgat aatccaaaca tagtcgttac gtttcggatt gtaaattcct tgaataaaat
     7861 tctttataac ttttccggtt tttaaagcga tcatagaggg gtgctcttcg ccaggcagtt
     7921 tattcatatt ctcatcaaca gcttgccagc gtggatcaat tgaggtaatc cctctcattt
     7981 tatcaaatgt aatgccaagt atctcttcgg cagctggatt ggcggagatt attgctccat
     8041 taacatcttg atagacaact ccttgggcca tagttgtgaa aaggttgcga tacctctctt
     8101 cacttaatcg taactgttta tgcgatttcc ggttctcggt cgtatcattg caataagcaa
     8161 gataaatatt gtcagatatt ttttgagcag caacattcca gtagcgatgt tcaccatttt
     8221 ttgctaaaaa tgagacttct ttctcataaa taccattctt aataacattc ttgaaatgtt
     8281 catagccttc ttttcttctg ttttggggga cgatatcaag tatagtcatg caaagaagtt
     8341 cctcacggct atagccagtt attttcgaag ccataggatt aacgtcaata tatcttcctt
     8401 ctttgtctga gacaaatatt gcgtatggag cgtattctat ataacttttc cattttagct
     8461 cgttcagttt tagtgcttct tcggtgtttt taacctcggt caaatcacga aattcaacag
     8521 ttcgaaccat cttgcctttg tatggaacat ttcgtgcttc aagtcgcatc gggaatattt
     8581 caccgttttt tcgacatccc tttgcttcat aagcttgttc atatcccgtt actattttgt
     8641 ttaatacata atcacggtct tcttcggcaa ttagcagaag accatccatt ccgataagtt
     8701 catcaattgt atatccggtc atcaaagata aaccgttatt acaatcaata atcacccctt
     8761 tgtcgtgtat tactatgcca ccaaaagagg cattatgcag tgctttaaag cgttcttcac
     8821 tctcctgaag ttccttttcg attcttttgt ggttttcgat ctccacttga agccgacatt
     8881 ttgcttcaaa caatttaaac gccattttga ttgatgcgtc cagcaccaca atgcctgaat
     8941 tcttaacaac atagccataa gatgtgattt tctctgtttt ctctacaatt gatggttccg
     9001 tatgggaaga aagaaataca attggtatct ccttgtcttt tacgatcttt tctgctgctt
     9061 cagttccatc cattccgtga cctaagtcaa tatccattaa aatcagatca agctgtttgt
     9121 cagtttcgag ggcagtttta atggcctcaa tcgcggtggc tacatgaata acagaatagc
     9181 catatttttc aagttcactt ttctctagtg tagccgttaa catctcgtct tcaacaagaa
     9241 gtatattttt gcctattcta ttcatatcag tttaatcccg gaatagattt tgttgtcgag
     9301 ttgcatggac ataatagggt aaaagggtcg actactgaaa gatatgaaaa taaacagtaa
     9361 agtcaaatat gatgaagtgt gtgccgtaaa aatatggatt tttaatagag tgattacaaa
     9421 tttatattaa tcgcaggttt ttagctttcc ctgaatactt aatccgaaga gagaaaagtc
     9481 atattttacc gggtcgtgcg ggtcaaactc ccgaagtttt agagtcagct cttctacagc
     9541 cagccaatca ttttgtttgc ggttaagtaa acccatgctt ctggctgcat ttccgcagtg
     9601 tacatcgagc gggatcatta atgccgaagt tggaatattc cataatccga aatgaacacc
     9661 gcgtccatca ttacggcaca tccacattaa taaaaggttc aatcttttag cggctgattt
     9721 ctttctaaca tcggaaatat gccttgtaaa gcggggaggg tgatttgaat taagaaaatc
     9781 atccctaaat tggattaagg tattgcgaat tgaaccttca gtattaaaaa cagactcgaa
     9841 ataggctcct agattgctat atttcatata aaatcttcgc aatgccaaaa tttcggtacg
     9901 gcaatcatcc gcgttaaatg tacggtgaac aaatccatct attttcttta actcattatt
     9961 tgaggcgttt aatacaaaat cataagggct gttatccata atgttcatca gattgtaacc
    10021 gtttctgata atcatgcttc tttttcccca agctatctgg gcggtcagaa aagctgaaat
    10081 ttctatatct tcctgttttt tgaaattatg gggtatttgt atagggtcac attcaataaa
    10141 ttcaggagtg ttgttgttta catattcagc ttcaaggagc tgagctattt tttgcttact
    10201 tttaaaatcc atgattaaca tttctgcttt gaaatgtata aatgcaataa aaaactttat
    10261 aatttaaaaa aacatgaaaa aaacttcgtt ttccggttat caagttaaaa aaaaatcata
    10321 tttagtagaa aaaatattag tatttattac tttaaattaa actattccaa atgtgtcact
    10381 tgcggcattt tgtatatcac ttattatgat aggtaaactt atataataaa aatactaatt
    10441 tataggaatt tttattgaaa actactattt ttgtgatata ttagtaatat cggcttgaca
    10501 atatactata aataatgtaa acaaaatgca ttgtatgtta aggaaaaagg ctattcattc
    10561 ttgatatttt gataattgcc atttaactat agaaatttca tttttatttt aaaattatca
    10621 aaaaaaacat aagcaaaaca ttgaaaatgt ttgcgaaaaa ttcttaaact ttaaaaaaaa
    10681 cttgctaaat aaaattaaaa ttctctaatt gtaataaatt atgataggtt cgctaaaagg
    10741 cagtatacag aaacttaaaa ccagttcttt gataattgat gtatccggtg taggatattt
    10801 cgtgagtata tctttgaata cgtattataa tctagcagac aaacaaactg tttttttgta
    10861 tatttatact tatattaaag aagacaagtt tgacctttat ggctttttga ctgaagaaga
    10921 aaaagatata ttcaaggtac ttatttcagt tacaggtgtt gggccatcgc ttgctcttgc
    10981 catcttgtcg ggtatgagtt ttactgaact gctaaacgta attcgaaata gggatgtagc
    11041 aatgcttacc aaaattccgg gtattggtaa aacaaaagct gaaaagatac ttcttgattt
    11101 gcaaagcaag gcaaaaaaac tgaatttgca aagtagtgaa acgataagcg gtggtaatcc
    11161 ttttttggaa gatgcagtag atgctcttgt cgccctcggt tttgaagaga gaaaatcaag
    11221 aattgctgtt gcggattgta ttggatcaaa taatcctgaa gacttacagg aaataattaa
    11281 gggttcgtta aagttattat aacagatttg aaattttttt aaagcatact attaaaaagg
    11341 agactattat ggatcaaaag aaaaagaaaa aagagagtgt aaatacaatc aaactgatta
    11401 ttcttggaat gttaatggaa cgtaatcttt acggttatga gataaataaa attatcagag
    11461 aaagatttca ggggtatgtt gatattaagt ttggttctat ctattatgca attaaagaag
    11521 ccattaaaaa gggttatgta aagaaatatg agacaattaa agataccccg acacctccaa
    11581 aagatatttt caaaattgag caggctggaa gaaaacagtt taagattctt ttaagaagat
    11641 atttttcgga aaatttcatc cattttgatg ttgatattgc attaatgttt ttaaattctc
    11701 ttactgatga tcaaaaagaa acatttattg aggaaagaaa agagctttta aaagagagac
    11761 ttgctgatat caaaaaagct ataaacggcg atgacggaag cgataaagat tatatgcata
    11821 tttatactta ccttgaaaat catttgaaag ctgaaaacaa ctgggttaaa agcctttaag
    11881 ttattcaata tcggtatttt tttcatatat tgcttttcct tgattatcag ggaaaagctt
    11941 tttttttatc ttttttaaga ttatgaacat gttttgagta tcaaattttt aaaaaccgat
    12001 ccagtttttc tcgaatatag tcggcattgt ggctgtataa cagctcatct acatggaatt
    12061 ttagtttttc aagatcatgg gatatgctac tgatctcttt tcgttcactt atttttcctt
    12121 cttcctcttc gagtttcttg tttaacaccg atgcattgat gtcacggatc aagcttatat
    12181 tgttatagaa tttcttagtt agcgagtata ctatttgtaa aatagtttca atatctgttt
    12241 tggctatcgt tttaagatca ttaagattta acttcgtaac gcgaacagga gtcaaagtct
    12301 taattgttgc gcttcttttt tcgcccaaaa gaagtgaaat ttcaccaata gtcgaaccaa
    12361 cctcatttat caaagcgaca cggttactgt ttactgtaac ctcaagagtt cctgattgta
    12421 aaatgaacat ttcattgtcg ttatcccctt ctttgcagag tatatgatca ggtggaagat
    12481 cgattgtctt ctcgcttaag gcctctgttt cataaatttt aactgggata tcggctttag
    12541 catatgattc tgcttttaaa aactgaattg aagaggtgta tttattaatt gtatctttta
    12601 accagggtaa cttcctttta ttatattcgt cgcgtagtat tgatactatt gaataatatt
    12661 gtgtaaatag ttgtttaagt gttttctttc ctgataaaag aatatcttgg tttttcttga
    12721 taatggaatt ggtaagtcgc agtttttgtg ccgtaacttt ggcaatgttt atcaagtatc
    12781 cttcttgctt aaattgctca agaaaagtat ctatctttac atattttaca tcggtatttt
    12841 gcggaactac cgctgtttcc acccttttgg taggaatacc tagcaaattt gatagaataa
    12901 tttcgctggt tcctataatc aggttttgcc cggttatccg gtaacgatca tcgtttgtgg
    12961 cgaaaaaaat cacttctccg gattgcataa tatatacagt atcaggatca tgtccctttt
    13021 cgtataatag tttgtaattc atataagact gttaacagca ttttgaaggt cttctttttt
    13081 aaagggcttt gtgacatacg cggttacgga caagtcacga ctgctgtttt gaagtgtttt
    13141 tttacttaat gccgatacaa caattatttt tgcatctgga ttgtccttct ttatatcctc
    13201 gattgtctgt ataccgtctt tcccatgaat agtgatatcc atagtgacaa tatcaggatg
    13261 taaatcgtta taaagagtaa tggcgtctac tccattgtcc gtttcggcga ctatcgtatg
    13321 gttattttcg attagcgtct cctttattat tgaacgcata aatttggaat catcaactat
    13381 catgatattt gccatagaaa tcctcttggt atgttatttt atagtttcaa gtatgttatt
    13441 aatgaaaaat cagaatatta cactgtaatc tgatttattt ctaaagaata tgaatataaa
    13501 gttataataa cttcaaataa taaatatatt aacagtgaac atgagtaaat atattaaaaa
    13561 tagtattata agccacacta actggtaaat agaaataaat aattcgaaat ttctttgtta
    13621 tttttccaaa taccgaccta actatatcgc tcttaaataa tattatattc aaatatcact
    13681 ccattatcaa taataataaa ataaacaaaa caaaaaaata gtcctattta aatatttcag
    13741 gcgtttttaa tgcgcaatat tttaaaaaaa ctcgtacagg aaaccctacc acattggata
    13801 ttgaaccatt tactgaagaa attatcaact cgggatgttc ctgaattgca tatgatccgg
    13861 ctttatcgtg ccaatcaata gagtttaggt acctacttat ttctgaattt gaaagttttt
    13921 taaaaattac ttctgttaca tcataatcgg atacaatttt caatgattct ttggcagggt
    13981 gagccattga aatggaggtt attacctcgt gacgttgacc gcttagtctt gtaagcatgt
    14041 tctctgcttc tgcacgattt tttggtttac cgagtatttt gttatctagg cttacaactg
    14101 tatcggctgt cactacaaag aaattatcag atgttacttt ttctttaata atccaggatg
    14161 ctttttcaga ggagattcta agagcataat caagagctgt ttcattagaa aacggggctt
    14221 cgtcaatttc tggagttatt tgctgaaatt cgtcaaaaag aagagaaaaa aaatagtggc
    14281 ggcgaggtga cgatgaacct aaaattataa tcattttcgt ttaattactt tttcttttag
    14341 tttatatact tcaactacat cttttacgct tttaagtcta tctataacaa aatgtaaatg
    14401 atccacactt tttacttcga caatcagcct aatgactgca aaacctgcct ttgactcctg
    14461 agcctcaagt ttcattatgt taattcggct ttgacttatc tctgtggtta tgtcagcaag
    14521 caaattaggc cggtcgatgg cttcgattgc tattttcaca ggatggacat gatgagaatt
    14581 agaccaagca atagagataa atctttccgg ttctatatta agccgtttaa gtgacggaca
    14641 tcctttttta tgtactgaaa ttcttttatc cttagagatg aatccgataa ctggatctcc
    14701 tggtattggt tggcagcatt gcgcgagccg tatagggaca ttgttgttat cttccaccag
    14761 aatacctgac ttcaggttat cgggagattt cgatattttt ggcatttcgg ccatatcgat
    14821 agtaaaagat gcgattttgt cgggctctga ttttgtatct tttttggact ttgatgatgc
    14881 ttcgtcagaa tcggttggct gctgtttttt tagccagctt cttattttat atcttgcgtt
    14941 tgatgatctt acaattttta gccagttttc ggacggatgt tttgccggat ttgttgttat
    15001 ttcaacaatg tctccgttgg ataattttgt tcgaaggggt tttatcttgt ggttaatctt
    15061 tgcacctgaa cagtgatgac caacttctga gtgtatggca taggcaaagt caataggggt
    15121 ggcacccttt gcaagcctta tgattttgcc tttaggtgta aaaacgaata tttcgtcgtt
    15181 atacaaatcc attttgagaa ctttcataaa ttcacgggta tcgtttagtt ccgaacgcca
    15241 tttattaatg ctcttcaata gtgagagatc agacatcttg gatttttctt ttttcccatt
    15301 ctctttatac atccagtgag cggcaatacc catttcagcg gttatatcca tctcttttgt
    15361 gcgaatttgt atctcaagaa aatgcttgcc ggggccggta acagttgtat gaagtgactg
    15421 atacatgttc gattttggta cagctatata atctttaaat cttgatggta caggcggcca
    15481 aatagtatgc actataccta gaatagcgta acaatctttt acctcttctg taattatcct
    15541 tatcgctcta acgtcaaata tctcttcaaa cggtttttgc tgatctacca tttttttgta
    15601 tatagaatag agatgtttga tgcgtcctga aatatcagct ttgatattca ttttacttat
    15661 atttgagtgc agggtggagt gtatctcgtt aagatattca tctagttctt cttcttgctt
    15721 agacagtttt gcagttatat ccaggaattc atcacggtgt agaatataaa aagcgagatc
    15781 ttcaagttca gaccttattt tggacatacc caggcgaccg gcaagaggtg catatatgtc
    15841 cattacttcc tgggcaatct tgagctgttt cggcttcggt tgaaattcaa gagtacgcat
    15901 attatgggtc ttgtctgcaa gcttaatgat aataactcgc ggatcatcta ttgttgcaag
    15961 cagcatcttg cgcagtgttt gtgcctgttc atgcgatttt gtacggtttt taagcgacga
    16021 aatctttgtt actccgtcta ctaggagagc aatttcggaa ccaaaggtgt cggctataga
    16081 gtcaagacca gtaacggtgt cctcgacaac atcatgaaga agtgctgcta taatggatga
    16141 tgagtctaga gaatgtttgg ctaatattat tgcaacgttt atgggatgga ttatataggg
    16201 ttcaccagaa agtcgttttt gatcacggtg agcagattcg gcaactgcaa aagccttttt
    16261 tataagggta acatctatat cgggatgctt tacggagatg atttgaagca aatcttcaaa
    16321 aagatcaaaa atatcattat agttatattc ggacattaat tgtgttcacc cgttttgaac
    16381 tgtcgctttg gagaatggct gctacgctaa aatataaaaa aagttatact cttaagactt
    16441 ctccacaaac agtattttct cattcacacc gttaaatata gtaacaaaag agtcaagagg
    16501 gaagtgttaa aaaaaatcat atttttttaa ttatacttaa gggatttaga aattgtccga
    16561 tattattaaa gattgattat catactccat gtaccatttg tttaagggct tgcgcggacg
    16621 gcactccggt gtaagccctt aaaacaaaat ggtttaacga cttatataat cgaaaatagc
    16681 tttgtcatac gaagacgtct ttttgaaaac tttgcatgca agctcgcgat ttgtatcaac
    16741 cgatactgac atttcgtttt cttccatctc tcgttctatt ctggtatagt cttctggatc
    16801 tgttatcact gtaaccgatt caaaattctt ggctgcgcta cgcaacatgc taggaccacc
    16861 gatatcaata ttttcgatag cctcttcaag tgtgacaccg gatttcgcca ctgttttttc
    16921 gaaagggtac agatttacaa ttatcatatc aatcggggtg ataccatgct gctccagagt
    16981 cttcatatgc tcaatgttgg agcgaactgc cagaagtcca ccatgaactt ttgggtgaag
    17041 agtctttact ctgccatcca ttatttcagg aaacccagta tgatctgaga tatccttaac
    17101 ggaaataccg ttctctttta aaagctttgc ggttcctcct gttgaaatga tttgtacgtt
    17161 gttttgcgat aaaaatcttg caaaaggtac aattccgtct ttatccgaaa gactgataag
    17221 agctgttttg attcgactca tatattacct cttttaacta atagtgccaa tgattccatg
    17281 tgtggcgttt gtggaaacat atcaaaaacc tcaaaacttt cgaggctgta ttgttctttg
    17341 agattctcat agtccgactt aaaagtgact gggttacatg acatgtatat tattcttgaa
    17401 ggcgaaagtg aatgcaggta tttatacatt ctcggattta tgcctgcccg agggggatca
    17461 agtattaatg tgtcaaaaga tttactattc tctttaataa atggacgcgc atcggcacat
    17521 acaaaagagg cattttcaat attattaagc tgcatgttct gctttgccga atttattgaa
    17581 tcttctacaa tatcaactcc tgttatactt tcgcattgat cagcgcaaaa caggctgatt
    17641 gaaccgacac cacaatatag atcaagaact cgacccgaaa cgagctctcg gattttccgg
    17701 tacatgttaa cagcaacttt tgaatttgat tggaaaaaag tatttggatg aattgtgaat
    17761 tttatcgatt tattattgtt atgagggctg ttttccagat caaagctttc tgtaatagaa
    17821 ccacctttta ttgtgtgtat ttccggaccg aaactgaggt cggtaagtga atcattaaga
    17881 agtattgatg atgagttaac caggctatca acagagttta aaattttctc aaatatctct
    17941 aaatcttttt tttcagatac aacaaaattt agcattgttt cacctgaaaa aaacccttgt
    18001 cttagtactg tgtatcttag aaatccgtgg tgagaaagat aatcgtaatc ctcaatctca
    18061 tggattgagt tgtgtattct cttccatact tcttcagagt tattttgaag aagtggacat
    18121 tcggtgatgt cgaccacctg tttgaatgat ccgcgtttac gaaaaccacg gcaaccaaat
    18181 gcagcaacat agtccattcg attgcggtaa ccaaagggtt ccgttgcatg tatattctct
    18241 attcttggca aaacatctgc acaaacctga ttcaaatatt gcgctttgat ttctaactgg
    18301 ttttcataag atatgtcctg aaaaaggcat ccaccgcatt caccaaaatg gctgcaaagt
    18361 gtttcacccg tccccctgta tttttcagct atatcaagaa taagctcctt gttttttctt
    18421 gttcgtttac ccattcaggc tccattatat ttaatttatc tttgcaacag ttacaatcaa
    18481 gatgtccaat aaagtataaa tcgataattt atagtatttg aaagattggt ttccttaatc
    18541 ggttattgat tattaatctt gctaaaattg ctgttggtaa atagatatgc actaagttta
    18601 atgattaaca atttaggata ttgtgaaaat ttatgtttat cagtaaagtc tttttattta
    18661 tggttcttga aatcgcggtt ttattgttta agggaatttc gaaaaatatt gtatctgaaa
    18721 ttactgtaag ttgagattat attactgaaa tatattgtgg gtatttgagg ttaagaatga
    18781 aagagagaac aagaaaagca agtattggtt cagtacagat tggaggaggg cttccagttt
    18841 tagtacagtc tatgtgcaat attcctctta gcgaaacaga cagtgtcata aatcagatag
    18901 gtcagttgca tgataggggt gcgcggttgg taaggttttc cgtgcgtaat atgaatgaag
    18961 ccgcctatat aagaaaagtt attaattctt cggaaattcc ggtatgcgcg gatattcatt
    19021 ttgactataa attagctatt gcggcaattg aagctggagt cgccaagata aggcttaatc
    19081 ctggaaatat taaatctgaa gatggagttc gtagggtagt ggaagctgct aagagtaatg
    19141 gaacggcgat acgtatcggt gtaaacggcg gctctgttaa ccgaacgggg ttcagcggat
    19201 cggaagaacg cattttggtt gatcatgcct ttgaacacct tagaattctt gaaaataatg
    19261 gtttctataa cactgttata tcaataaaat catctgatgt tctttcaact ttaaaagcaa
    19321 acaggcttct tgctacggag tgcgattatc cgattcatat tgggcttacc gaagccggtt
    19381 atggtaataa cgcactgata aaaagctctg taacaatagg agcattgctt cttgacggaa
    19441 ttggcgatac agtcagggtg tcaatgactg gagatccttt gcaagagatt gacgcggcat
    19501 atgagatact caaggctgta caccggcttg attatggaat taatattata tcgtgtccaa
    19561 cttgtggacg aactgataga cttattgatt tggaggcatt agcccgagat attgacaaag
    19621 cagttaatgc caagtatttg aaattagtaa gggatcaaaa aaagattctc aatattgccg
    19681 taatgggatg tgaagtaaac ggtccgggtg aggcatccga agcagatatt ggagtagctg
    19741 gttgtggtcg tggacagctt gccctatttt tgcgtggaaa gataataaag aaggttaatc
    19801 aggacagggt tgtagaccag attttggaat acacgggcaa aatagtggcc ggtattagtt
    19861 taccggccga ataggtctat tttgtattaa attgttacca ttctattttg tcttttttgc
    19921 actggttttc tttgctgttt tcttagtgtt ctttctgctt gatgaatcag atttattttg
    19981 actggttttg cctgctggtt ttatcttggc tatagagctt gatttctttg cagcaggctt
    20041 ttttgatctg ttttttgttt tagcctgaag aacaagagcg gtgtcactaa ggtgataatg
    20101 aaaatctgag ttttcgacta ttctatggtc atgtgtcaca attacaaagg ctttgttatt
    20161 ctccttggcg agttttataa aaaggttgtt tatatcttct gctgactcgt catcaaggtt
    20221 cccggtaggt tcgtctgcaa gaataatgtc gggattattt ataacggctc ttgcgatagc
    20281 aattcgctgt ctttctcctc cagaaagtgt ttctggatga tttgaagaaa ttgctttaag
    20341 gttaagatac tcaattagtt cttttgcttt ttttgaggca tttcttgcaa aagggttaaa
    20401 cattgttggc agaagaatgt tttcaaaagc actataatcg ttaagaaggt taaaaaactg
    20461 aaatataaag ccaatgcttt tgcttctata aaacgaacgg cgtatatcgc cccaccttag
    20521 aatgtttttt cctttaaagt aaaccttgcc gtcattgggt tttagcaatc cggagattat
    20581 tccaaggagg gttgttttgc cgcatcctga ttttccagtt attactgtaa tagttcccgg
    20641 ttccacagaa aaattaacat cgctaagaat catccttgaa ccgaatttca gtttaatgtt
    20701 ttcgacttta agtagtgata tgttctgttt gttttttttc attaaaatac cctatcgtct
    20761 cactgcttta aatagattta tgttagatgc aaatatagct ggaagtagcg ccgaagaagc
    20821 ggaaacaaga gcagataatc ccatagctat cataaatact tccttgttac gttgagcaac
    20881 aaattctata cctgctatat ccggtaaaaa tgaagatatt gattcggctg cataagagaa
    20941 gaggtaattg cccgcgtagt atccaatgat tccatagatc gcaccgacaa ttgctgcttc
    21001 tacaataaaa gtgataatta tccgtatttt ggttactccg agtatacgag ttaatgaaaa
    21061 tttatgactc atatggtaaa cgacattcag attggcgtta aagatagata caatcgttaa
    21121 aagaataaga attcctacaa tagttaatga aaaagcatcg ataattttca ttacctggtt
    21181 tgctttatcc gcgattccac gatcagactt aacctgtaaa ccgagttttt ctagtttatc
    21241 aacggtttcg ggtaattttt taatatcctt aacctttgca taaaattgaa gataatggta
    21301 gccgcgtttc catctaccag aggagacctc ggctcgtttg gaaaaatatg ttataaaatc
    21361 cataggcaca atggctccaa tacgttcggt gacattggtg aaactgaata cagatacatc
    21421 cagatcagta tgcgtttttc ccttgtcttc tttgaattct attttggctc cagggaaagg
    21481 tcgtggagat ttgagagttt cgatctgtat ttccatggga aagcctttca ggtcctcaat
    21541 tttaaattgt ggaagcccat tgaaatagag gtagttattg agcatgacca atgtaaattt
    21601 tggcgctatc attttaagag gtcggccagg tttgtagtaa aactgatccc atttgggaat
    21661 tttgttttta aaaaactcgg gttctatcgc gaaaagagga aggatcattg tgcttctgtt
    21721 gccaaacatc tctccacgaa gcctgacccg gtaatcaagc ataattaaag taaaaacttc
    21781 tgaaaaatcg tccatttctc gaacggcttt gacttgatca attgtcagtt tcgcgccctg
    21841 tcgtgatgaa gctgcataaa cattgtccgc tttttcatta atcggaagaa tttctatctc
    21901 gtcgatttca agtgaaccta acagattttc atcaataaaa tcactaatcg atgtacgaaa
    21961 agagagataa ataattagaa atgtaataat aaaaagtata ccgaagcttg aaaaaaaaga
    22021 ggtaattatt gatttgaatg aactgcgtaa accgtaataa attgagcgta atagatgtac
    22081 cataattaat cctgatttga tttttattca attatttgtc tttatgcctt taaagacata
    22141 ttcgtaaaca tttaacattt atagagaact tcgaaaatta ctgtgttaat gagagaaatc
    22201 gtcattaaat tacatgttta ttgaaaatta tcgcaagttc ctagttaaaa tattaattat
    22261 taaaggttcc ctataattaa aaaaatattt gtaaacagta aaaaaagaaa aatt
//
LOCUS       DAOTGZ010000017        21146 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_3064
            NODE_2998_length_21146_cov_6.866768, whole genome shotgun sequence.
ACCESSION   DAOTGZ010000017 DAOTGZ010000000
VERSION     DAOTGZ010000017.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868088
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 21146)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 21146)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:22:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,785
            CDSs (total)                      :: 2,751
            Genes (coding)                    :: 2,732
            CDSs (with protein)               :: 2,732
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 12 of 19
            Pseudo Genes (internal stop)      :: 5 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21146
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2998_length_21146_cov_6.866768"
                     /isolate="AMR_MDS_3064"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(170..1804)
                     /locus_tag="P5123_02395"
     CDS             complement(170..1804)
                     /locus_tag="P5123_02395"
                     /inference="COORDINATES: protein motif:HMM:NF024883.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HRX15141.1"
                     /translation="MLMTTDSVVLYSEQIAGMESAKILVVDDEPSVRFTLVKFLTLKG
                     FRVIEASNGQEAFDMLYRYSFDLVITDLQMPIADGRFLMKKMAESFPNIPRIILTAHE
                     EEDDLLLALKTGAYDFLKKPIPDLSLLVHSVNRALERKKLHDERSNTIKQMEHINEVV
                     SMLNQGKSIEEIFTMLTESLNHIIAFNRITVFDFFAESGVLSVRLSKSDRSEKLKVGE
                     ELIIRGKVLDKIMKNREVVIIPDLHAFFASDDYPSIFYKVLTEGFTSAIITPLITEDS
                     VHGFLVLASVNLGYYTAEDARFLTAIAGQISFSLERGRLLSELSTHTRHLEKMIQIRT
                     FEIMKTQKITIFALSKLAEIRDNETGEHLERMRNYSLLLSQLLKYSGNYPEINNEFLK
                     NIYDSSILHDVGKVGIPDAILLKPAALTPNEFDMMKTHTSIGYSALDNASRSLGADSF
                     LDMSKDIALYHHERWDGKGYPSGKTGKDIPLCARIVTIGDIYDALTSKRPYKDEFPHQ
                     KAVEIMQQEFYRFDPGIFQIFMENNNEFERIQKQFS"
     gene            complement(1847..2590)
                     /locus_tag="P5123_02400"
     CDS             complement(1847..2590)
                     /locus_tag="P5123_02400"
                     /inference="COORDINATES: protein motif:HMM:NF012321.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="vWA domain-containing protein"
                     /protein_id="HRX15142.1"
                     /translation="MARKAVIILIFFMFFASSAYAASEGVDLVIVVDQSKSIKKNLPV
                     IKDFIAKRIFDQIAQPQDTVYLLSFDGQFYFKRKLSGSSNIFDIGYALDSVQAVGGYT
                     DITNAVIEMSSFIDKQTDPKRKKIVFFLTDGLNEPPSYSKYPSKLDPALFAETLHLKK
                     DGGWSVFITGIGAKTSAPDLAQILEGKYAQISSAPTLEEFDTNITQQLKIVRKRTGNP
                     FSATVVATVAIAIAAASVGGVALFRKYKV"
     gene            2883..3374
                     /locus_tag="P5123_02405"
     CDS             2883..3374
                     /locus_tag="P5123_02405"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRX15143.1"
                     /translation="MKQFFKKPSLLILVMTIFTAVAFTSCDDLLDKAKDAAEDALTSD
                     VTAKCEGSYNASVIGLDDYGFRFCDNYTQETDLRSAEDDCGTLPGTKYTADATCNKAD
                     ALAKCEVTTVDKVKYTKYYYVSSGSYDPSEFSTVNSALEKHCEDVLGKLGDAEYTFLA
                     EKE"
     gene            3830..6115
                     /locus_tag="P5123_02410"
     CDS             3830..6115
                     /locus_tag="P5123_02410"
                     /inference="COORDINATES: protein motif:HMM:NF024754.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UvrD-helicase domain-containing protein"
                     /protein_id="HRX15144.1"
                     /translation="MSSNLNQNQQKAVTTTEGPLLILAGAGSGKTRVITYRIAHLIRE
                     KGVPPYSIFAVTFTNKASAEMKERVSELIGPSGDQVFVKTFHSAAVYILRRWGNAINI
                     PSSFSIYDSSDQESVIKELLRKRNVDPKKVKPSAIANQISSVKDKAELLTGADISALL
                     PDHYGFNFVELYEEYQSELRARNALDFNDLLIMTYKLLNESSETLEHLQNKWRYFMVD
                     EYQDTNRAQYLIAKLLAAKSRNICVVGDDDQSIYSWRGADIRNILSFEDDYPEAEIIT
                     LNENYRSTSPILEAAHSVIQNNTERKDKRLVAVRGEGEIPVYCTVNNEYGEAEFVINT
                     INTLKSQENFNNRDFAIFYRTNAQSRIFEDFLRRSNMPYRLIGGQKFYDRKEIKDVMA
                     YLRFTANNLDTVSLLRIINTPARGLGAKTIETLRNIAYINQLSEWETIDRNIPLGKKQ
                     PKGIVQFQKLMRKLIDLKNRIKDQQAKLSDLAHCAIEDSGYLASLESSDTPENKMRIE
                     NIKELVNSIYEYEQRAEIPSLEEFLQEVSLLTSEENPNHNEMYDPDNCITMMTVHNAK
                     GLEFPVVFLTGMEEGMFPHWNSSETDEDIEEERRLAYVGITRAMNRIFLTAAQFRRSY
                     SGELSYKECSRFLDEIPAHLLLTKEYTENSSSGFASREANRFMSKSSSNFSSYKKEGS
                     VSARSKIENYKNNNLSKASLSTNSSSSAFSQRQRVRHPKFGEGTIIAITGSGDNVKLT
                     INFSGVGLKSFLEKYTPLEAV"
     gene            6120..7979
                     /locus_tag="P5123_02415"
     CDS             6120..7979
                     /locus_tag="P5123_02415"
                     /inference="COORDINATES: protein motif:HMM:NF013790.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRX15145.1"
                     /translation="MTISEIVEKLIAAKEAYYETSSPIMSDSDFDSLEDELKNLDPQN
                     PYFGIVGTGSQDNKIRHKVPMLSMQKAKTVDEVQKWMEKLAIDNQTGYCIQPKIDGLS
                     ASCCYENGRLLYVATRGDGATGQDITHIADHVQDIPENIEFTKNRIEVRGELYLPKDT
                     KYDTQGRPLRNNCVGLINRKESREELHFVRFAAFQIICENLVETESEAIELLSVNGFN
                     TVSTQIALSINQIDEYFSKYLIELRNSWKYETDGLVITVNNRNLFEDIDSRWVVDHHH
                     HYAIALKPPAEAKETKLIRVEWQISRQGNAIPVAVFDPVFIGGARIERASLSNYETVN
                     KMGISSGNRLLIERANDVIPYVRENLDLDESSEPGSDLIISNCPSCQTKLEEQGVHLH
                     CPNKECPETSIQKILFWVRESGIEQIAEATIRSLYNKKIIHNIKDLYNIKEDDLKGVE
                     GFAQKKISSFLTEVSTSKRMSAREFIAKLGIPLVQKKALAKLKIFSMKDFYDFDDPSY
                     IIGQNIISWKKNDTNISLVKDLSEALDIIDENAADTKPKVCMTGKGPLPRKELISEIE
                     NMGYEFSETITSDTSILLCENSNGTSSKLQKARKNGIKLINYSDFFVNNMKSN"
     gene            8147..9154
                     /locus_tag="P5123_02420"
     CDS             8147..9154
                     /locus_tag="P5123_02420"
                     /inference="COORDINATES: protein motif:HMM:NF012742.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000976 - transcription cis-regulatory
                     region binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HRX15146.1"
                     /translation="MGEKTVTISDIAHIAGVSKSTVSRALNDSPLISDKVKENIHEIA
                     LQHNFQFNQAARNLSRQKTDTLAFLFPHYRGSDHYTKQPFPMELLRGIIFATNKYGYN
                     LLLSESNFESWRDSYKYVNSKQADGLIIISPDPHDNTIIELIEKDIKFVTWGIPENKG
                     YCTVNSNNLDGGLIATEHLIKKGRKKICFLGMEEDNFETVMRFLGCDHATQQSNINVC
                     SSCIAYGTNSQSSGYENMKKIIQQQPDIDGVFCCSDLIALGAIEALTEYGKSVPNDVS
                     VIGFGDLPLAQMVTPKITTIRNDIWRAGEQMVENLDHCLKTGEIRNSIIPVELVIRET
                     T"
     gene            complement(9170..10507)
                     /locus_tag="P5123_02425"
     CDS             complement(9170..10507)
                     /locus_tag="P5123_02425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019668533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M48 family metallopeptidase"
                     /protein_id="HRX15147.1"
                     /translation="MKKLVPLLIIICFLATEFLFASVSDNSVLAKLWTPENIFWAIVI
                     FITFDFVLELALDILNYRNMRPELPEELKDIYDEEKYAKSQLYEKARFKIGLIKSIFS
                     YVLIMTVLVINGFAIADNISRSLVEHPVFTALVFFSILYIVSDILSLPFQIYSVFSIE
                     ERFGFNKMTPALFVADKIKGYLLSALIGGGLLSLIIFIYLYTGSMFWVYAWGLVTTFT
                     VFITMFYSTLIVPLFNKQRPLPEGELRTAIEEYSKNAGFKLDNIYEIDGSKRSTKANA
                     YFSGFGFKKRIVLFDTLIKEMTTGEIVAVLAHEIGHYKKKHVLISMVMGILQTGAILF
                     IFSILAKSPQLSAALGVQQHSFHIALITFGILFTPINLVLGMALNVFSRKNEYEADAF
                     AGKSYDANELASALKKLSVKNLSNLTPHPAYVFFHYSHPPVLKRLNALKAIKK"
     gene            complement(10563..10982)
                     /locus_tag="P5123_02430"
     CDS             complement(10563..10982)
                     /locus_tag="P5123_02430"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRX15148.1"
                     /translation="MNIFILSVQFEGEDALEAFGDITRTLRQNLKTLQSGNYTDDLKK
                     LKIYLGVGGSLGDFVEPNGCHAVTIHGETCIVDVVMHKEIWEKGKKSIVQFLADQVKR
                     AFVKIVNTMKADNLMIRDERLLRDVNRAVDLWLKNYL"
     gene            complement(11106..14168)
                     /locus_tag="P5123_02435"
     CDS             complement(11106..14168)
                     /locus_tag="P5123_02435"
                     /inference="COORDINATES: protein motif:HMM:NF016292.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate binding protein"
                     /protein_id="HRX15149.1"
                     /translation="MEIILVISRRFAHFVITASIVFYGNILFSMEKNILVLHSYHPSY
                     VWTDSVQKGIESVFPLTSSKVLLSVEYMDTKRFTLEEIAPTFATSLKVKYVSKDFDAI
                     IIADNNALTFFHDQRDTLFKDVPAVFCGINDYTDDMLKNDSLLTGVAEKTNSLGTLKL
                     MQKIFPERQKIVVIVDTTVTGEAEYQRFKRYSKEIEGLDFETWMDYSPDKIIQGLSRL
                     DDNCMVLSIIYLQSSDGSYVNFSSYLEMISKESPVPVFALWDFYFQKENAAFGGDIVS
                     ASDQGQLAAQMAKQIIEGVSVKDIPVIKGNTSIPVIDYRQLTKFRIAKGAIPKNVTYL
                     FDQDNFLNEKLYQFIRTILLVSVVGALCVLVMINIYSRRKTEKSLIDAQNTLAALFEN
                     IPGMAYRSKSENGFPFLFVSRGSTHITGYHAKELNDRTLLDLIVHDDSETVNDVQKEI
                     DKSINERTPFELEYRIRKKDGSVISVWNHGIYNNNGKFIEGLILDISDRVAANMAMQE
                     SEGWFQKILNSHVDGIVIHEHDTLRTLDVNDSALEMFGYEKAEFTKMYLSDIVSGNPP
                     YTTESFLYRYKITDDLRNQQFECEVCKKDGSLFWCEIFVKSIEINDNKYILSFIRDIT
                     ERKKSRMQLKEKEEQLRHAQKMDAIGQLAGGIAHDFNNMLGGILGAAELMLIDPQDSE
                     LVKEYARLIIDTTAHASDLTENLLAFSRKGKVLSTPVDCHKIIEDTIKILKRSIDRRI
                     VLQKDLRAQKTMIIGDPGQIQNIVLNLGINARDAMPDGGRLSIATSNVTLDKQKCESS
                     GFDMHEGDYLCITISDTGTGIEASVIDKIFEPFFTTKTIGAGTGLGLAAAYGSVTEHK
                     GSISVSSKKGEGSVFTILLPLSDKNRVSCDEVVGEVPLGKGTVLLAEDESFVRNTAVH
                     ILEDLGYTVIIAENGKDAFHLYKIHREKIDLVLLDMIMPGYSGEECFYKIREIDPFMP
                     IVVCSGFAHEQDISKMTEEGLNGFLLKPYRRFELAQMLYKVLNKEDCV"
     gene            14312..15028
                     /locus_tag="P5123_02440"
     CDS             14312..15028
                     /locus_tag="P5123_02440"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="GGDEF domain-containing protein"
                     /protein_id="HRX15150.1"
                     /translation="MKVHKKILLSVNNPEIVNILKKLLEPEFEISVSDNNTSDSADII
                     ISDNSSFVLPDPDINSVTGLPGLNTIISSLKKMLFENKSHTVIACNINHFKQYNAAQG
                     YEQANALLKFLADLVKNGIIKSGSSKNFIGHAYNDFFISIIDSDKAVTVSDYIITRFD
                     EYISEQGNTSTNSNSDSFMNREGDIKPLPKTSIRLAGVDINKYEPEQYLLVLNTIDAL
                     LQRAKLEEYSCIFFDRRTKP"
     gene            15332..16576
                     /locus_tag="P5123_02445"
     CDS             15332..16576
                     /locus_tag="P5123_02445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015235192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HRX15151.1"
                     /translation="MENLSVLQKQYEDLIAEGLNLNIERGQPADENFDIANPMLTIVD
                     DNDLVTPSNISIRNYPGGPAGIPEIRQICGDLLGIKSEETIVGNNSSLAMLVNTLMWA
                     LIKGINSSSSPWITQKPKIIVTVPGYDRHFTLLEALGFEIITVPMEGDGPSVDKIEQL
                     ASSDSSVKGLIFVPTYSNPTGETISDKKLIHLAEMKTAAEDFTIFADDAYTVHHLSDE
                     YDEAKNIIRECEKAGNPDRAYVFGSTSKITFSGAGIGFMGSSINNVNYICKLLTAQSI
                     GPNKVEQYRHYKFFKQFPGGVEGLMMKHAQILKPKFDAAQKVLNEELGGKELADWTNP
                     NGGYFISLDTRYPVAKRVVELCKKAGVALTPAGATFPQGNDPKNSNIRIAPTRPSVED
                     VTKAMKVLCACIKLASAEHLEN"
     gene            16867..17733
                     /locus_tag="P5123_02450"
     CDS             16867..17733
                     /locus_tag="P5123_02450"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012472.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HRX15152.1"
                     /translation="MIEKMEFGRTGHKSTRLLFGAAAFYNTTDPDDVKKTMDTVDRFG
                     INHIDTARGYNKSEEMLGPWLKHNRERFFLATKTEARSFKDAMTDLEKSLDFLKTDHV
                     DLWQMHCLAEPEEWEKARDEENGAIRAFREARDKGLVRFLGVTSHGMKAPVVLKSAVD
                     YFDFDSVLLPYNYPIIQDNAYNKAFCQLYEDCKKRNRAIQIIKTIAIGPYPDAEGQTY
                     NTWYKPLVSQSDINTAVSWAMNLENSFINSAAEMSLLEKIAQAADTNRSPVTDEQMRQ
                     LAEKTGMTNLFA"
     gene            complement(17846..20761)
                     /locus_tag="P5123_02455"
     CDS             complement(17846..20761)
                     /locus_tag="P5123_02455"
                     /EC_number="6.1.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015869067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0004832 - valine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006438 - valyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="valine--tRNA ligase"
                     /protein_id="HRX15153.1"
                     /translation="MKENLATVYNSSEIEKDALKSWSESDSFAADPSKPGDSFAIVIP
                     PPNVTAALHLGHALNNTLQDIMIRKNRMEGKNTLWMPGTDHAGIATQTVVEKRILAEE
                     GKKRTDFEREEFVARVQQWKDEYEERIINQLKAMGCSCDWNRTRFTMDKVCAKAVREI
                     FFRLFDDGLIYRGKRQVNWDPATQTVLADDEVEHETIEGFFWYLKYPLVDPVEINGEK
                     ITHITVATTRPETMLGDTGIAMNPSDPRAEKLTGKKVRLPIVGREIPIVADLHVVLPD
                     PESSDEKARFSTGFLKVTPAHDPYDWEIGVRHNLQVINIMAPDGSISDSHGWDDAVGT
                     EGEQFVGMDRFEARAAIVDWFRNENLLEQVKEYAHDVGHSYRSHVPIEPYLSDQWYIA
                     VKKPVDRLKDKFGTDKIGETEIAQNSLAGMALKPLLDEKLTIIPDRYNKNYRVWLENL
                     RDWPISRQLWWGHQIPVWSKAGKHENIFGDAVRLLVFDSAEGPVSYICVKSENEAIEK
                     EIESQGWVRDNDVLDTWFSSALWPFSTLGWPDENESLKKYYPGSVLCTAREIITLWVS
                     RMVMMGQYVLGEIPFKDVFIHAMIQDGEGRKMSKSLGNGIDPLEIIDSHGADAMRFAL
                     AAMTTMTQDVRMPIEKMTLPDGREVNSSPKFDIGRNFCNKMWNASRFAMANLENIAEI
                     DKTELEIEDVWILSRLHKTIASVTTQIDEYRFSDPINGLYRFFWNEVCDWYLELIKPR
                     MWDDNRKGSAQRVLAYVLDCSLRMLHPFLPYITEGIYQKLNGFMSERSIPGVADLKSS
                     ELLITAEWPKANFGFYNDETEKQMELLQDIIKMIREIRTKYQIKPKDKVKVTVNVSGE
                     KSVILSDNERLISNIAGLEEFIVTEKLDSKPERSAVAVGEGIELFVHNVVDTEAEKQR
                     LNKQKDKLIQEIERAEKKLGNENFISRANPDVVEKEKEKLSVLKEQLEDIEKNLQSL"
BASE COUNT         6617 a         4450 c         3788 g         6291 t
ORIGIN      
        1 tctatcacat aatatcagtg tatacaatca accatttttg tttcaaaaaa agtaaaatcg
       61 tggctcattt tacctgtttc agcatgaatg gcaagggttt aacaattagc actgctattc
      121 ttaaaaaaga attatatgta agcaaaaaaa gacctgttta ctgcgagatt tacgaaaact
      181 gtttttgaat ccgctcaaat tcattgttgt tttccataaa aatttgaaaa ataccgggat
      241 caaagcgata aaactcctgc tgcataatct ctaccgcctt ttgatgggga aattcatctt
      301 tatagggtct ctttgaagtt aaagcatcgt aaatatcacc tatcgtgacg attcgtgcgc
      361 atagaggaat atctttgcca gtttttccgc tagggtagcc tttcccatcc cagcgctcat
      421 gatgatacag ggcgatgtcc tttgacatat caagaaacga atccgcccca agacttcgtg
      481 acgcgttatc caaagccgaa tagccgatac tggtatgggt tttcatcata tcaaattcat
      541 ttggtgttaa tgcggcaggt ttaagcagta tcgcgtctgg aattccaact ttgccaacat
      601 catgaagaat ggaagagtca tagatgtttt tcaaaaattc gttatttatt tcaggataat
      661 tacctgaata ttttaacagc tgcgataaaa gcagagaata gtttcgcata cgttcaagat
      721 gctcgccagt ttcgttatcc ctgatttcgg ccagtttgct taacgcaaaa attgtgattt
      781 tttgagtttt catgatctca aaagttctta tctgaatcat tttttccaga tgacgcgtat
      841 gcgtcgaaag ctctgacaag agccttcccc tctcaagact aaaagaaatc tgacccgcaa
      901 ttgcggtcaa aaaccgggca tcttcggccg tataatatcc gagatttacc gaagccaaaa
      961 ccagaaagcc gtgaacagag tcttcggtaa tcaatggagt aattatcgct gatgtaaagc
     1021 cctcggtcaa aaccttgtaa aatatggaag gataatcatc agaagcaaaa aacgcatgca
     1081 aatcaggaat aatcacgact tcgcgatttt tcatgatttt atctaaaact tttccacgga
     1141 taatcagctc ttcaccaacc ttcagttttt cagatctgtc agattttgac agacgaacag
     1201 agagcacacc cgattctgca aaaaaatcaa aaactgttat tctgttaaag gcaatgatat
     1261 ggttaagcga ctcggtaagc atggtaaata tctcttcaat gcttttgccc tgattaagca
     1321 tcgacaccac ttcattaata tgttccatct gcttaattgt gtttgaacgt tcgtcatgca
     1381 gtttttttct ttctaatgca cggtttacag aatgaacaag aagtgataaa tcaggaatag
     1441 gttttttcaa aaaatcatag gcacccgttt ttagggccaa gagcaggtcg tcctcttctt
     1501 cgtgggcagt caagattatg cggggaatat taggaaacga ttcggccatt tttttcataa
     1561 gaaaacgacc gtcggctata ggcatctgga gatctgtaat aaccaagtca aacgaataac
     1621 ggtaaagcat atcaaaggct tcctgcccgt tagaagcctc aatcacgcga aaccccttca
     1681 aggtgagaaa ctttacaagc gtaaaacgca ccgaaggctc atcatcaact acaagaatct
     1741 tcgcgctttc cattcccgca atctgttcac tgtataaaac aaccgaatcc gtagtcatta
     1801 gcattaccaa gttgtcctgc tgaaatttaa aaaaatttta tctttttcac actttatatt
     1861 ttctgaaaag agctacaccc ccgacactag ccgccgctat cgcgatcgct acagttgcta
     1921 ccacagtagc cgagaaagga ttaccggttc tttttcgaac aattttcaac tgctgggtga
     1981 tatttgtatc aaattcttca agagtaggtg cggaacttat ttgagcatat ttgccttcca
     2041 atatttgtgc aagatcggga gcactcgttt ttgcgccaat accggtaata aagacgctcc
     2101 aaccaccatc tttttttaga tgaagcgttt cggcaaacag ggcaggatct aacttgctgg
     2161 gatacttgct ataagagggt ggctcgttaa gcccatctgt taaaaaaaac actatttttt
     2221 tacgtttggg atccgtttgt ttatctataa aactgctcat ttctattaca gcattggtga
     2281 tatcggtata accacctacc gcctgaaccg aatcaagggc gtagccaata tcaaaaatat
     2341 tcgaacttcc actcaatttt cgcttgaaat agaactgtcc atcaaaagag agcaggtaaa
     2401 ccgtatcctg aggctgggca atctggtcaa aaatcctttt tgcgataaaa tccttgatta
     2461 ctggcagatt tttcttgata cttttactct gatcgacaac gattacaagg tcgacaccct
     2521 cagatgcagc ataagccgaa gaggcaaaaa acatgaaaaa aatcagaata ataactgctt
     2581 tcctagccat taacacactc ctggacggtt gaaagaccat tttccttact gtaggggttt
     2641 ttaagacctt tcattataac taacttttat ggaaatgcaa gttattaatc tgatcagacg
     2701 gtaaaatttt cagaaaaatg gcctattatt ccactataaa ctgaaaaatt tcttttagta
     2761 acaattaatt tttaatggtt cttaatattc ttcttgcaaa acagcagtga tgagctatat
     2821 ttattcaata gcttagtatg cgttttttta tacgcaaaca gcataattta aggagactaa
     2881 ttatgaaaca gtttttcaaa aaaccaagcc ttttgattct tgttatgact attttcacag
     2941 cagttgcctt tacttcatgt gacgacctgc ttgacaaagc aaaagatgca gcagaagacg
     3001 cgcttacaag cgatgttaca gcgaaatgtg aaggcagtta caatgcaagc gttatagggc
     3061 tcgatgatta cggattcaga ttctgtgata actacacaca ggaaactgac cttcgcagcg
     3121 ccgaagatga ttgcggtacg ttaccgggaa caaaatacac tgctgatgca acctgtaata
     3181 aagcagatgc tcttgcgaaa tgtgaagtta caactgtaga taaagtcaag tacaccaaat
     3241 actattatgt atccagtggt tcttatgacc cgtccgagtt ttccacagta aatagtgctt
     3301 tagaaaagca ttgtgaggat gttctgggca agcttggtga cgctgaatac acatttcttg
     3361 cagaaaaaga ataaaccgaa aaaaccataa aacaactcac tatccgcgga agaaatctat
     3421 tcttccgcgg tttagttttt cagattatct ttacctcctc tctttttatg aaaatcagac
     3481 ctgatttatc gatcttggcg ggggtactga atataattct tacctgttgt gtcatgtctc
     3541 aaaaaaacat tttcatcggc acccaagcat caaaaatgga cttctgcttc aaatgctgct
     3601 ccatttataa atatttgaac ctcagataat taactaataa tcaagggact tacaaataat
     3661 cttttttaag aaaaaaatca gaagaaatat cttctattag tgcataattt gtgcatacca
     3721 taatattcat ggtaattttt gtaattttcg tattaataaa tttgacacca ttttaccggt
     3781 taaaccataa tctttaagat aactgagccg acaaaaggag caggcattaa tgagtagcaa
     3841 tcttaatcaa aatcaacaaa aagcagttac aacgactgaa ggccccctat taatactggc
     3901 gggtgccggt tcgggcaaaa ccagggttat cacatacaga atcgctcatc ttatcagaga
     3961 aaaaggcgtg cctccctact ccatctttgc tgttactttt acaaacaagg cctcggccga
     4021 gatgaaagag agggtctccg aacttatagg cccgtcgggc gaccaggttt tcgtaaaaac
     4081 ctttcattcg gccgcggttt acatattgcg gcgctgggga aacgccatta atatcccatc
     4141 atcgttttct atctatgact cttctgatca ggaatctgta attaaagagc ttcttcgcaa
     4201 aagaaatgtc gatcctaaaa aggtaaaacc gtcggctatt gccaatcaga tatcttcggt
     4261 taaagacaag gcagaacttt taaccggtgc cgacatttcg gctctgttac cagatcatta
     4321 cggatttaac tttgtcgaac tttacgaaga ataccagagc gagcttaggg ctcgtaatgc
     4381 cttggatttt aacgacctgc ttattatgac ctataaacta ttaaatgaga gtagcgaaac
     4441 gctggaacat ttgcaaaaca aatggcgcta tttcatggtg gacgagtacc aggatacaaa
     4501 ccgcgcacag tatcttattg ctaagctgtt ggcggcaaaa tcgcgcaata tctgcgttgt
     4561 gggtgatgac gatcaatcaa tatattcatg gcgcggagcc gacatacgca atattttatc
     4621 ttttgaagac gactatcccg aagccgaaat aatcacactt aacgaaaact accgctcgac
     4681 ctctcctatt cttgaagcag cccactctgt tattcaaaac aataccgagc gcaaggacaa
     4741 gaggcttgtt gcagtaagag gtgaaggaga gattcctgtt tactgtaccg ttaacaacga
     4801 atacggagaa gcggaattcg ttatcaacac cataaacacg ctgaagagcc aggaaaactt
     4861 taataaccga gactttgcaa tattttacag aacaaacgcc caatcacgta tctttgaaga
     4921 ttttttacgc cgttcaaata tgccgtaccg gctaataggt ggacaaaaat tctacgaccg
     4981 taaagagatc aaggatgtaa tggcttatct gcgttttaca gccaataatc ttgatactgt
     5041 atcgctatta agaataatca atacacccgc tcgtggactt ggcgcaaaga ctatcgagac
     5101 tcttcgaaat atcgcctata tcaatcagct tagcgaatgg gagaccatcg accgcaacat
     5161 tcctcttggt aagaagcagc ccaagggaat agttcagttt caaaaattaa tgcggaaact
     5221 tattgacctt aagaacagaa tcaaggacca acaagcaaaa ctttcagacc ttgcacattg
     5281 cgctatcgaa gacagcggtt atcttgcttc gcttgagtct tcggacactc cggaaaacaa
     5341 gatgcgcatc gaaaacatca aggaacttgt gaattcgata tatgaatacg aacaaagagc
     5401 agaaatacca tctcttgagg agtttctgca agaggtatct ctgcttacat ctgaagaaaa
     5461 cccaaaccat aacgagatgt atgatcccga caactgcata accatgatga cagttcataa
     5521 tgccaaggga cttgagtttc cggtcgtttt tttaaccggc atggaagagg gaatgtttcc
     5581 tcactggaac tcatccgaaa ccgatgaaga tatcgaagaa gagcgccgtc tagcttatgt
     5641 cggtataacc agggcaatga accggatctt cttgaccgcg gcacagtttc gcagaagcta
     5701 ttcgggagag ctttcataca aggagtgctc ccgctttctt gacgagatac ccgcgcacct
     5761 tctcttgaca aaagagtata ccgaaaacag ctcttccggt tttgcttcac gtgaagcaaa
     5821 tcgctttatg tcaaaatcat cttctaactt ttcttcgtac aaaaaagagg gttctgtttc
     5881 ggcaaggtca aagattgaaa actataaaaa taacaacctt tccaaagcga gcctctctac
     5941 caattcgtca tcaagcgctt tttcacaaag acagagggtt aggcatccaa aatttggcga
     6001 aggcacaatt atcgcgataa caggcagtgg cgataatgtt aaacttacaa ttaacttttc
     6061 cggtgttggc ttaaaatcct ttttggaaaa atatactcct cttgaggctg tttaaaacca
     6121 tgacaatatc tgaaatagtt gaaaaactga ttgccgctaa agaggcatat tacgaaacat
     6181 cttcgcccat tatgagcgat tccgattttg attcactcga agacgagctt aaaaatcttg
     6241 atccacaaaa tccatacttc ggtattgttg gaaccggttc tcaagacaac aagatacgcc
     6301 acaaagttcc tatgctatcg atgcaaaagg caaaaacagt tgatgaagta caaaaatgga
     6361 tggaaaaact tgcaattgat aatcaaaccg gctattgcat acaacctaag attgacgggc
     6421 tctcggcaag ctgctgctac gaaaacggca ggctgctata tgtcgctaca cgcggcgacg
     6481 gcgctaccgg acaggatatc acacatatag cggatcatgt acaggatata ccggaaaata
     6541 tagaatttac taaaaaccgg attgaggtta gaggtgagct ttatctgcca aaggacacga
     6601 aatacgacac acagggccgg cctcttagaa acaactgcgt tggccttatt aaccgcaagg
     6661 agagcaggga ggaactgcat tttgtacgtt tcgcggcttt tcagataatt tgcgaaaatc
     6721 ttgtcgaaac agaatcagaa gccattgagc ttctttccgt aaatggtttt aatactgttt
     6781 cgacacagat agccttatca ataaatcaga tcgatgaata ttttagcaaa tacctgattg
     6841 aacttagaaa ttcgtggaag tatgaaactg acggtctggt tattacagta aacaacagga
     6901 accttttcga ggatatcgac tcacgctggg ttgtagacca ccatcaccat tatgctattg
     6961 cacttaagcc ccccgccgaa gctaaagaga caaaattaat cagggtagag tggcaaataa
     7021 gtcggcaagg caacgccata cctgttgcgg tatttgaccc cgtatttatt ggtggagcac
     7081 gtattgaacg ggcttctctt tcaaactatg aaacagttaa taaaatgggg atatcctctg
     7141 gcaaccgtct gttaatcgaa agagccaatg acgtaattcc atatgtcaga gagaaccttg
     7201 atcttgatga atcctcagag cccggctctg accttatcat ttctaactgc ccttcatgcc
     7261 aaaccaaact tgaagaacag ggagtccatt tgcactgccc caacaaggag tgtccggaaa
     7321 catccataca aaagatactc ttctgggtaa gggaaagcgg catagaacaa atagccgagg
     7381 ctaccataag atccttgtat aataaaaaaa ttatccataa cattaaagat ctttataata
     7441 taaaagaaga tgatcttaaa ggagtcgaag gttttgctca aaagaaaata agttcttttc
     7501 ttaccgaggt atcaacgtct aaaagaatga gcgcgaggga atttattgcc aaactgggta
     7561 ttcccttggt gcagaaaaaa gcactggcta aacttaaaat tttttcaatg aaggattttt
     7621 atgattttga tgacccctcc tatatcattg gtcaaaatat tattagttgg aaaaaaaacg
     7681 acacaaacat aagccttgta aaagatcttt ctgaagcgct tgatattatc gatgaaaacg
     7741 ctgctgatac aaaaccaaag gtttgtatga ccggcaaagg accccttccc cgcaaggagc
     7801 taattagcga aatagagaac atgggttacg agttttcaga gactattaca tctgatacgt
     7861 caattttatt gtgcgaaaac agcaatggaa caagctccaa acttcagaaa gcacgaaaaa
     7921 atggcattaa actgataaat tattctgatt ttttcgtaaa caatatgaaa agcaactaat
     7981 atgaaaatct ccttgatttt ttttcctgtt ttttgataaa tcataatatc acacaatcgc
     8041 ttgaatagta tacttaaatc gggatcaatt ttccataata accttttaac agaagcgatt
     8101 gttattatac cgttactcag ataaacgtat aaacaaggag ccttttatgg gcgaaaaaac
     8161 agtaacaatc tcggatattg cccatatagc cggggtctcg aaatcaaccg tatcacgggc
     8221 tcttaacgat agccccctga taagcgataa ggttaaagaa aacatacacg aaattgccct
     8281 tcagcataat tttcagttta atcaggccgc aagaaactta agtcgccaaa aaaccgacac
     8341 acttgctttt ctttttccac actatcgggg aagcgatcac tatacaaagc aaccttttcc
     8401 aatggagctt ttacgcggaa ttatttttgc tacaaataaa tacggatata atctgttatt
     8461 aagcgaatca aacttcgaat catggagaga ttcgtataaa tatgtaaact ctaagcaagc
     8521 tgacggatta ataattattt ctcctgatcc tcacgacaat acaattatag agcttataga
     8581 aaaggatatc aaatttgtta catggggtat tcccgagaac aagggttact gtactgttaa
     8641 cagcaacaat cttgacggtg gccttattgc aaccgagcat ctgattaaaa agggccgcaa
     8701 aaagatctgc tttttgggaa tggaagaaga taatttcgaa accgttatgc ggtttcttgg
     8761 atgtgaccat gcaacacaac agagcaatat taacgtttgc tcttcctgta tcgcttacgg
     8821 aacaaattct caatcttccg gttacgaaaa catgaaaaaa atcatacaac agcaaccgga
     8881 catagacgga gttttctgct gcagcgacct gattgccctt ggagcaatcg aagctcttac
     8941 cgaatacggg aaaagtgttc cgaatgatgt gtcagtaatc ggcttcgggg acctgcccct
     9001 cgcacagatg gttacaccaa aaataaccac tatccgcaac gatatctgga gagccggtga
     9061 acagatggtg gaaaaccttg atcactgtct taaaacaggt gaaatacgaa actccatcat
     9121 cccggtagag ctggttatac gagaaaccac atagttaaac caaacccctc tattttttta
     9181 tcgcttttaa agcattaagc ctttttaaaa caggcgggtg cgagtaatga aaaaacacgt
     9241 atgctggatg cggtgtcaag ttgctaagat ttttaacaga cagctttttt aatgcagaag
     9301 caagttcgtt ggcgtcataa gactttcccg caaaagcatc ggcctcgtac tcattttttc
     9361 tggaaaaaac atttaaagcc attcccagta caagattaat cggtgtaaac agtataccaa
     9421 aagtaataag cgcaatatga aaactgtgtt gctgtacgcc taaagccgcc gaaagctgag
     9481 gactttttgc caaaatagaa aagataaaaa gaatggctcc tgtttgcaat atgcccataa
     9541 ccatagaaat taacacatgc ttctttttgt agtggccaat ctcgtgcgcg agaactgcaa
     9601 ctatttcacc tgtagtcatc tctttgatca gggtatcaaa aagtacaatt cgctttttaa
     9661 atccaaaccc ggagaaatag gcatttgcct ttgttgaccg ctttgatccg tctatttcgt
     9721 aaatattatc aagtttgaat ccagcgtttt tgctgtactc ttcaattgct gtgcgcaact
     9781 caccttcagg caaaggccgc tgtttattaa acaaaggaac gatcaatgta gaatagaaca
     9841 tagttataaa aacagtaaaa gttgttacaa gtccccacgc atatacccag aacattgagc
     9901 cggtataaag atatatgaat ataataagtg aaagcaagcc tccaccaatc aaagcactta
     9961 acaagtatcc ctttattttg tcggctacaa aaagagcggg tgtcattttg ttaaatccaa
    10021 acctctcctc tattgagaaa accgaatata tctgaaaagg caaagataaa atatctgaca
    10081 cgatatatag tatgctaaag aatacaaggg cagtaaacac aggatgctca accagagagc
    10141 ggcttatatt gtccgcaatg gcaaaaccgt taataacaag aaccgtcatt attaaaacat
    10201 aactgaatat acttttaatc agtccaattt taaaacgagc cttttcataa agctgtgact
    10261 ttgcatactt ctcttcatcg tatatatctt tcagctcttc aggaagctct ggtctcatat
    10321 tgcgataatt taaaatatct agagccaact ctaacacaaa atcaaaagtg ataaaaatga
    10381 ctatcgccca aaaaatattt tccggtgtcc ataatttggc taaaactgaa ttatccgata
    10441 cagacgcaaa aagaaattcc gttgctaaaa agcagattat tataagcaaa ggcactaatt
    10501 ttttcattac tattcccttt cgtattttta tttttaacta catctctata ttattaagca
    10561 tgttataggt aattttttaa ccacaagtca acagcccggt ttacatctct taaaagtctt
    10621 tcatccctta tcattaaatt atcggctttc atggtgttaa caatctttac aaaagccctt
    10681 tttacctgat ccgctaaaaa ctgaacaata gacttttttc ctttttccca aatctcttta
    10741 tgcataacga catccactat gcaggtttct ccatgaattg ttactgcatg acagccatta
    10801 ggttcaacaa aatctccaag gctgccgcca acaccaaggt aaattttaag tttttttaaa
    10861 tcatcggtat aatttccgga ttgtagcgtc ttaaggtttt gccgcaatgt tctggttata
    10921 tctccaaagg cttccagagc atcctctccc tcaaactgaa cagataatat aaaaatattc
    10981 atttctgcat cctgattcaa gaataggggc tttaactttc aagttctttt gcgatttcat
    11041 acttaatcat aaaaattgtt gatgcaagaa taattttata ttcttgctct attttatgtt
    11101 gttcttcaca cacagtcttc tttattaagc actttgtaaa gcatttgagc cagctcaaaa
    11161 cgcctgtacg gctttaacag aaacccgtta agaccctctt cagtcatttt acttatatcc
    11221 tgttcgtgag caaaacccga gcatacaacg attggcataa atggatcaat ttctcttatc
    11281 ttgtaaaagc actcctctcc actgtaccca ggcataatca tatccaaaag aacaaggtca
    11341 atcttttccc tgtgaatctt atagagatgg aacgcatcct ttccattttc ggcgattatt
    11401 actgtgtaac caagatcctc cagaatatgc acagctgtat ttcttacaaa agactcatct
    11461 tcagccaata aaaccgtacc cttgccaaga ggaacctcac caacaacctc atcacatgat
    11521 actctatttt tatccgataa aggcaacaaa attgtaaaga ctgatccttc tcccttcttg
    11581 ctactgacag atattgaacc cttatgttca gtaacagatc catatgcagc cgccaaacct
    11641 aatccagttc cggcacctat ggtttttgtt gtaaaaaacg gttcgaatat tttatcgatt
    11701 acggaagctt caatacctgt tcctgtatca gaaattgtta tacagagata atccccttcg
    11761 tgcatatcaa atcccgaact ttcgcacttt tgcttatcaa gagttacatt tgatgtcgcg
    11821 attgaaagcc ttcctccgtc aggcattgcg tcacgagcgt taatacctag gtttaatact
    11881 atattttgta tctggcctgg gtctccaata atcattgttt tttgtgcgcg cagatctttt
    11941 tgtaaaacaa ttcgcctgtc tatacttcgt ttaagaattt ttattgtatc ttcgattatt
    12001 ttatgacaat caacaggtgt agacagaact tttcccttac gactaaatgc cagcaaattt
    12061 tctgtcaggt cagacgcgtg agcagttgta tcaattatca atcttgcgta ttctttaaca
    12121 agttcactgt cctgcggatc tatcaacatt aactcggcag cgcccaatat tccaccaagc
    12181 atattgttaa aatcgtgcgc aattccacca gccaactgac caatagcatc cattttttga
    12241 gcatgccgta gctgctcctc tttttctttt aactgcattc gggatttttt tctttcggtt
    12301 atatcccgaa taaatgaaag tatatacttg ttatcgttta tttcaatact cttaacaaat
    12361 atttcacacc aaaatagtga tccgtctttt ttacaaactt cacattcgaa ttgctgattt
    12421 ctaagatcat ctgtaatctt gtacctgtaa agaaaactct ctgttgtgta gggcgggttt
    12481 ccacttacaa tatcagataa atacatttta gtgaattcag ccttttcata accgaacatt
    12541 tcaagagcac tgtcattcac atcaagcgtt cttaacgtat catgctcatg tatcacaata
    12601 ccatcaacat gactattcaa tattttctga aaccatccct cactttcttg cattgccata
    12661 tttgcagcaa ctcggtcact aatatccaga ataagtcctt cgataaattt tccattgtta
    12721 ttataaattc catgattcca gaccgatata accgacccgt ctttttttct tattctgtac
    12781 tcaagttcaa aaggagttct ttcgtttata cttttatcta tctctttctg tacatcattt
    12841 acggtttcgc tatcgtcatg aacaataaga tccaaaagag tgcggtcgtt taactctttt
    12901 gcatgataac cagtgatatg ggtagagcct ctgcttacaa acaagaatgg aaatccattc
    12961 tctgattttg atctgtaggc catgcctgga atattttcaa aaagtgcagc cagagtgttt
    13021 tgtgcatcta tcaaagattt ttccgtttta cgccgcgagt aaatattaat cataaccaaa
    13081 acacataacg ctccaacaac actgacaagt aatattgttc ttatgaattg gtataacttt
    13141 tcgttcaaaa agttatcttg atcaaaaaga tacgttacat tcttcggaat cgcccctttt
    13201 gctattcgaa attttgttaa ttgtctgtaa tcgattaccg gaatagaagt atttcctttt
    13261 atcaccggaa tatcttttac agaaacacct tcaattatct gctttgccat ttgtgccgca
    13321 agctgtccct ggtctgaagc cgatacaata tcaccgccaa aagcggcatt ttctttttga
    13381 aaatagaagt cccaaagcgc aaataccgga acaggggatt ctttgcttat catctccaaa
    13441 taactgctga aattgacata acttccatca ctactttgaa gataaattat agacaaaacc
    13501 atacaattat catcaagacg gctaaggcct tgaatgattt tatccggact gtaatccatc
    13561 catgtttcga aatctaaacc ctctatttct tttgaatatc ttttaaatcg ctgatattcg
    13621 gcttcaccag ttacggtagt atccacaatc acaacaattt tttgacgttc aggaaatatt
    13681 ttttgcatca gctttaatgt tcccagactg ttggtctttt cggcaactcc agttaaaaga
    13741 gaatcatttt taagcatatc atcggtatag tcatttattc cacaaaaaac tgcaggaaca
    13801 tccttgaaaa gggtatctct ctgatcatga aaaaaagtca aagcattgtt atctgcaata
    13861 attattgcat caaagtcttt agagacatac tttactttta gtgatgtcgc aaatgtaggt
    13921 gcaatttctt ccaaggtaaa gcgttttgta tccatatatt cgacagacaa caatactttt
    13981 gaagatgtga gtgggaatac ggactctatt cctttttgga cactgtctgt ccatacatat
    14041 gaagggtgat acgaatgaag aaccagaatg tttttttcca ttgaaaacaa aatatttcca
    14101 taaaacacaa tagaggctgt aataacgaaa tgtgcaaaac gcctgctgat taccaaaata
    14161 atctccataa atcaatgctg gatatcgaca aactactatt tattgtataa gatatatttg
    14221 caacatattt aggtttgaca taaacgaaat taacagctat tttactatca atagggaatt
    14281 tttaaaactt ccctattccg gagaaaataa aatgaaagtt cacaaaaaaa tccttttatc
    14341 cgtaaacaat ccggaaattg ttaatatttt aaaaaaatta ttagagcctg agtttgaaat
    14401 atcggtctct gacaataata cttctgattc agccgatatt ataatctctg acaacagcag
    14461 ttttgtttta cctgatccgg acattaactc tgttactgga cttcctgggc taaacacaat
    14521 aatttcgtct ctcaaaaaaa tgctttttga gaacaaatcg catacagtga tagcgtgcaa
    14581 tattaatcat tttaagcaat ataacgctgc tcaaggctat gagcaggcaa atgctttgtt
    14641 gaaatttctt gcagatcttg taaaaaatgg cattattaaa tccggttctt ccaaaaactt
    14701 tatcggacat gcttacaatg atttctttat cagcattatt gactctgaca aagcagttac
    14761 ggtttccgac tacataatca cccgttttga tgagtatata tccgaacagg gcaacacttc
    14821 tacaaacagt aattctgact cttttatgaa tagagaaggc gatattaaac cacttcctaa
    14881 aacaagtatt cgtttagcag gtgtagacat caataaatat gaacccgagc agtatttgct
    14941 agttttgaat acaatagacg cccttttgca aagagcaaaa cttgaagaat acagttgtat
    15001 cttttttgat cgccgcacca aaccttagat aaattttgag ttttatttaa ttaatgctat
    15061 tttttgttgc aatatgctaa cactttttca cattgacttt aggtttaaaa aattgctatt
    15121 tgtcaattaa acggatcata accggattaa tatctgttta cactttaagg ccggttatat
    15181 atgtctgact ttactcttcc gactgtcgct cttgcgactt ccacgatatt tataaccgcc
    15241 agaccgtaat aaacagattt tttttaaaac tccggttcaa ttaattcagt taattatttt
    15301 aaaaaataga cccaaaattc tggaggaatt catggagaac ctatctgttt tgcaaaaaca
    15361 gtatgaagac ttgattgcgg aaggccttaa tctgaacatc gaacgaggac aacctgccga
    15421 cgaaaacttt gatatcgcta accccatgct aactattgtt gatgacaatg atttggtaac
    15481 accctcaaac atttctatcc gcaactatcc tggaggacca gccggaattc cggagataag
    15541 acaaatctgt ggagaccttc tgggcattaa atctgaagag accattgtag gcaacaactc
    15601 cagtcttgca atgcttgtga atacactgat gtgggctcta attaagggaa taaacagcag
    15661 ttcttcgccc tggattactc aaaagcccaa aataattgtt actgttccag gttatgaccg
    15721 ccattttacc cttttggaag ctttaggatt cgagatcatt actgttccca tggagggtga
    15781 cggaccatca gtagacaaga tcgagcaact ggcttcgtct gattcttcag taaaaggcct
    15841 gatatttgtt ccgacataca gcaaccccac aggtgaaaca atatccgata aaaaactgat
    15901 tcatcttgca gaaatgaaaa cagccgctga agattttaca attttcgctg atgacgccta
    15961 tacagttcac catctttctg acgaatatga cgaagcaaag aacataattc gcgaatgcga
    16021 aaaggccgga aaccctgata gagcttatgt tttcggatct acaagcaaga ttacattcag
    16081 cggcgccgga atcggcttta tgggatccag cattaataac gtcaactata tctgtaaact
    16141 gcttacagct cagtctattg gacctaataa agttgaacaa tatcgacatt ataagttctt
    16201 caaacaattc ccaggtggtg ttgagggttt gatgatgaag cacgctcaaa tattaaaacc
    16261 aaaatttgac gctgctcaaa aagtattaaa cgaagaactt ggcggcaaag agcttgcgga
    16321 ctggactaat cctaatggcg gatattttat cagccttgac acaagatatc ctgttgccaa
    16381 aagagttgtc gaactctgca aaaaagctgg ggtagcatta acgcccgctg gtgcaacctt
    16441 tccacagggg aacgacccaa aaaactcgaa tatccgtatc gcaccaacac gaccaagcgt
    16501 agaagatgtt accaaggcta tgaaagttct ttgcgcgtgc atcaaactcg caagcgccga
    16561 gcatctagaa aattgatatt ctgttataaa gaggtgtcct ttacaggtca cctctttata
    16621 atttattttt ttcaacaatt tcagtttagc aatcctgatt gttttactct tagtcgaatg
    16681 cgccaactag taatattttc aagtcactaa atgtaacatt atatagccaa ttaaaaaact
    16741 gttgaaaaat ttgacgattc tgcattttca aagaccggtt tatcatcctt gacggctaat
    16801 ttgagagaga gtatattact tacttgtgta aagattaaaa gaaaacatca aaaagaggct
    16861 cttgtcatga tagaaaaaat ggaatttgga agaaccggac ataagagtac acgactgctt
    16921 tttggagcag cggcatttta taacacaact gatcctgatg atgtaaaaaa gactatggat
    16981 actgttgacc gctttggaat aaaccatatt gatactgccc gtggatacaa taagagcgaa
    17041 gagatgctcg ggccgtggct aaaacataac cgtgaaagat tttttttggc aacaaaaaca
    17101 gaagccaggt ccttcaagga tgcgatgacc gatcttgaga aatcactgga ttttttgaaa
    17161 accgaccatg ttgacctttg gcagatgcac tgtcttgctg aacccgagga gtgggaaaaa
    17221 gccagggacg aggaaaatgg agccatcagg gctttcagag aagccagaga caaggggttg
    17281 gtacgatttc tcggtgtaac aagccatggt atgaaagccc ccgtcgtgct taagtcggcc
    17341 gtggattact tcgattttga ttcagtacta cttccataca actatcctat tattcaagat
    17401 aatgcttaca acaaagcttt ctgtcagtta tatgaggact gcaagaaaag gaacagggca
    17461 atacaaatta taaaaacaat tgccatagga ccatatcctg atgctgaagg ccagacatat
    17521 aacacatggt acaaaccact tgtcagtcag tcggacataa acacagctgt gagctgggcg
    17581 atgaaccttg agaactcatt tataaacagc gccgcggaga tgtcactgct agaaaaaatt
    17641 gctcaggccg cggacaccaa tcgttcaccg gtaaccgatg agcaaatgag gcagcttgcc
    17701 gaaaaaaccg gcatgacaaa tctatttgcc taaaatttca ccaacactat aagcccttat
    17761 cgaataatct ttccagcagg aataatcttg cagattttta ttaatcttag cttgttagta
    17821 acagaactaa caagctaatc atctattata gcgattgaag attcttctca atatcctcaa
    17881 gctgctcttt caatacagaa agcttctcct tctccttttc aacgacatcc gggttagcac
    17941 gtgaaataaa attttcattg ccaagtttct tttcggcccg ttcaatctcc tgaatcaatt
    18001 tatccttctg tttgttcagt cgctgttttt ccgcctcggt atctaccaca ttatgaacaa
    18061 aaagctctat accctctcct acggcgacgg cagaacgctc tggttttgag tccaatttct
    18121 ctgttactat aaactcttcc agcccggcaa tgttagaaat taatctttca ttatcactaa
    18181 gtattacaga tttttctcca gatacattaa ctgtaacctt aactttgtcc ttcggtttaa
    18241 tttgatattt agttcttatc tcgcgaatca ttttaattat gtcctgaagc agttccatct
    18301 gtttttctgt ttcatcatta taaaaaccaa agtttgcttt tggccactcg gctgttatta
    18361 acaattcgga tgacttcaga tcggcaactc caggtatgga tcgctcactc ataaaaccgt
    18421 taagtttttg atatatacct tcggtaatat atggtaaaaa cggatgaagc attcttaatg
    18481 aacagtcaag cacataagcc agaacccttt gagcacttcc tttgcggtta tcatcccaca
    18541 ttctgggctt aattagttca agataccagt cacaaacctc attccagaaa aaacggtaaa
    18601 gtccgttaat aggatcactg aatctgtact catcgatctg tgtagtgaca gaagcaattg
    18661 ttttgtgaag ccttgagagt atccagacat cttctatttc cagttcggtt ttatcaatct
    18721 cggcaatatt ctcaagattg gccattgcga aacgtgacgc gttccacatc ttgttgcaaa
    18781 aatttcgtcc tatatcaaat tttggagagc tgttaacttc tcgcccatct ggtaaagtca
    18841 ttttttcgat aggcatgcga acatcctgtg tcatggtagt catggccgcc agtgcaaatc
    18901 tcattgcatc agcgccgtga ctgtctatta tttccagagg atctattcca ttacccaaag
    18961 actttgacat ctttcggccc tctccgtcct gaatcattgc gtggataaaa acatctttaa
    19021 acggaatctc acccagtaca tactgcccca tcattaccat gcgtgagacc cacagagtaa
    19081 taatttctcg tgctgtacaa agtaccgatc ccggataata tttttttagc gactcgtttt
    19141 catcgggcca tccaagtgta ctgaaaggcc aaagggccga gctgaaccaa gtatcaagta
    19201 cgtcgttatc ccttacccag ccttgtgact cgatctcttt ttctattgct tcgttttctg
    19261 attttacaca aatatagctt acaggccctt ctgctgaatc gaaaaccaac aatcgtaccg
    19321 catcaccgaa tatgttttca tgcttgccag ctttactcca aacagggatc tgatgtcccc
    19381 accacaactg acggcttatt ggccaatcac gcaaattttc caaccacact cggtaattct
    19441 tgttgtagcg gtcaggaatt attgttagct tttcatcaag aagcggtttt aacgccattc
    19501 ccgcgaggga attctgtgct atctcggttt cgccgatttt atctgttcca aatttatctt
    19561 tcagtctgtc aactggcttc tttacagcta tataccactg atctgaaaga tacggctcga
    19621 taggaacatg gcttcgataa ctgtgtccga catcatgggc gtactcttta acctgttcca
    19681 aaagattttc atttcgaaac cagtccacaa tcgcggctct tgcttcgaac cgatccatac
    19741 caacaaactg ctcgccttcg gtaccaacag catcatccca accatgagaa tcgctaattg
    19801 atccgtccgg agccataata ttaataacct gtaaattatg acgaacaccg atttcccagt
    19861 cataggggtc atgggccggt gtaactttta aaaatcccgt gctaaatcga gccttctcat
    19921 cactgctttc tgggtcaggc aaaacaacgt gaagatctgc aacaattgga atctcgcgac
    19981 caacgatagg caaccttacc tttttacctg tcagtttttc cgctcgaggg tcagatggat
    20041 tcatcgctat accagtatcg cccagcattg tctctggtcg agtagttgcg acagtaatat
    20101 gtgttatttt ctctccgtta atctctacag gatcaaccag aggatatttt agataccaga
    20161 aaaagccctc tatggtctca tgctcaactt catcatcagc caaaacggtc tgtgtcgcgg
    20221 gatcccagtt aacctgtctt tttccacgat aaatcaaacc gtcatcaaaa aggcgaaaga
    20281 atatttccct tacggccttg gcgcaaactt tatccatggt aaaacgagtt ctattccagt
    20341 cacagctgca tcccatcgcc ttaagctggt ttataatacg ctcctcgtat tcatccttcc
    20401 actgctgtac tctggcaaca aactcttccc gttcaaaatc tgttcgcttt ttcccttctt
    20461 ctgcgagtat tcttttttcg acaaccgttt gggtggcgat ccccgcgtga tcagttccgg
    20521 gcatccataa tgtgtttttt ccttccattc ggtttttacg aatcattata tcctgaaggg
    20581 tattgttaag ggcatgccca agatgcaatg ctgcagttac gttcggtggt ggaataacaa
    20641 ttgcaaacga atcacctggt tttgaaggat cagcagcaaa gctatccgat tctgaccaac
    20701 tttttaaagc atctttttct atttcgcttg aattatagac tgtggctaaa ttctctttca
    20761 tatatatctt cctggttagt ttgtatagtt acctattttg acaacagtct tatatagata
    20821 agtttttaga caattaaatt tttaaaaaat agctaggatt gacaaatgca aacaatctag
    20881 ttcattatgc ttggcataac catgaaatat tcacatgatt tatcatggtt tttttaactt
    20941 tctctttaaa actgtaaaaa ttgattgttg attatattta taatgcttct tatattttac
    21001 caagccctag tagttgactt taattaagcg ataatagatt ctaaaataga tgattttcga
    21061 tgtttttttg agaaacattg agttattttt cgatatctaa aagagtggtg ttcttaatta
    21121 ttagctatat taatttatgg agaatt
//
LOCUS       DAOTGZ010000018        20307 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_3064
            NODE_3189_length_20307_cov_5.912157, whole genome shotgun sequence.
ACCESSION   DAOTGZ010000018 DAOTGZ010000000
VERSION     DAOTGZ010000018.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868088
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 20307)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 20307)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:22:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,785
            CDSs (total)                      :: 2,751
            Genes (coding)                    :: 2,732
            CDSs (with protein)               :: 2,732
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 12 of 19
            Pseudo Genes (internal stop)      :: 5 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20307
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3189_length_20307_cov_5.912157"
                     /isolate="AMR_MDS_3064"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(67..1638)
                     /locus_tag="P5123_02460"
     CDS             complement(67..1638)
                     /locus_tag="P5123_02460"
                     /inference="COORDINATES: protein motif:HMM:NF012894.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015288 - porin activity [Evidence IEA];
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HRX15154.1"
                     /translation="MFLRITGLLLLLMFFSLEAGIYEVPGHDIKSYEADTEILRKKIR
                     KEPSVPEHSKNLILFLMSIEKFDDAVKEADIHLAKNSDQEIEYCRIIALASSSQYDKA
                     DNALRKYINKYKHSKEERKLLNDRRRMYKRALETKGELLGLDIVSDEEEVLGVDKSKA
                     ALITYNRRLKTVMLRSLFDAKSAGRLTLPKNTDYDDIINISISSNGKSVLASFRKGSK
                     AVIKESSFDRGWKDWTESKELNEGDLNSYPCFIKGSSEILFVSNRKGSSGLDVYYSKK
                     TRSGWSKPKSVEGANTISDEASVFATGDSDLVFVSSNGRPGLGGFDIYSGVLNLDSTP
                     SIAINRNVVYVNSYRNEVAPPYYDIKESNFFITHNNADRTTIYKADKLWKDAIKYEPV
                     KEPEIKEPEVKMPETGKLVAHDLRFDTGSAYIRPSSYKFLEQLVKYMRDNPHKIVVID
                     GHTDNVGTEDFNIKLSLERAESVAKYLERNGIPRERIKTNGYGTSKNIDTNSTEEGRQ
                     RNRRVEISFIDKVSK"
     gene            complement(1629..3860)
                     /locus_tag="P5123_02465"
     CDS             complement(1629..3860)
                     /locus_tag="P5123_02465"
                     /inference="COORDINATES: protein motif:HMM:NF019335.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015267 - channel activity [Evidence IEA];
                     GO_process: GO:0015891 - siderophore transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HRX15155.1"
                     /translation="MRYLIAIFFIFACGTLMAGTVSGKVTDTAGRPVAGLVVGLDNNF
                     YTAETASDGSFVFENVEPGDYYLHLSNATWGHHRFGIRVKSNFRAVFEISKKQNSADP
                     VELNEVPPLFDTRYRISNEQITRYPMRGIGDSMHLLQTIPGVGGGYSLASVPIIRSTN
                     PLLNKYYIDGIPINNPYHFALSFAPVISAISDPAIDQVVLYKGDAPVRMGDSSGNVID
                     IRTAEPSQAGIQGKVILDGILPILPTVAVMGVIDPRWSVVAVGRRSTSDILLDVEDNN
                     YFLQDYYLKTTYVLNDSHRFRFIANGAGETFKWDDALSESGYSLFALQWDYRINRIFY
                     LETSLSRYQINRELDIDVENGASIVFDPVEYRVYQSLSAGMGSFAVSGGYEYTSYSNG
                     VVSNISMEGLATPDILSSEAETESTEFAIDGYGLRFFADFSARFDRVWFLAGVNYDKF
                     EVQNNSDFGYQAELGFNAYRTTSVYLKAGRFIAHPDMIYYLGAVDADSENPEMVTPAQ
                     LKNTITETYSLGVISYDLLKTRLQAEFFFTTHQNLFPGGSIITVNDDSYRKAAQLHKF
                     ALEDDGYTYGLELGANRSFLRRYNATVAYTFQSSTRNLEDDNRYVELFGDNAKEINSE
                     YNQSHILRLMADARFGRWVPALLFNAYSSLPYTEVTGSREIEGLGTQTLYGKENDARY
                     SMHHRLDAKVNYYASDDLRFYAEIWNIYLNRENQIADYKNRSHREVYDLPVFFWMGME
                     LCF"
     gene            complement(3865..4308)
                     /locus_tag="P5123_02470"
     CDS             complement(3865..4308)
                     /locus_tag="P5123_02470"
                     /inference="COORDINATES: protein motif:HMM:NF015506.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="energy transducer TonB"
                     /protein_id="HRX15156.1"
                     /translation="MMTLKNIFYFMLFMLFFSCGSSFYQTRDNVSGNTEVLNYEYSNL
                     YYSHKLKKEHFSLNIELNKPAVVTLSNIDFKPVERHSPVIGASLVRVTVDKNGRVINY
                     SIVKYAGAGLDGYLESVIRSSKFRPVEHKEEPQECDFILKVVFRN"
     gene            complement(4322..5146)
                     /locus_tag="P5123_02475"
     CDS             complement(4322..5146)
                     /locus_tag="P5123_02475"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRX15157.1"
                     /translation="MCLNLLRFVILSGLIILLGSFSAFCDIQRGGYVLGSFSPIGLSP
                     GNRVLYDYEPKGSVDASTESDITSLMDYSLEAGYFHGLLMADARFYSTSVDNQKIEYS
                     EYFRDETISYKINTLDVRAGLRLSDLGNSSFTYPYIGYRYFSYSSDYLDTQLKGHGFT
                     VGLTGFYTFFPRFDFEPALYVNAYLGNYRFTDLESDMTSDFERSKSIAAGATFGVGVH
                     YEPYNVTMLAIVRGSIDQISHDAKADDGWVECGIRHTGFYAGLAVMYQRYNFKYNR"
     gene            5350..5847
                     /locus_tag="P5123_02480"
     CDS             5350..5847
                     /locus_tag="P5123_02480"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRX15158.1"
                     /translation="MNKNIISIIVAMAILIPALIASYITFTNIRDEIRLNLFGKISTA
                     DVTSHRMRPVTADKIVYELTYNFTIDGNEFSMSKDSDDQNWASLPKKQWENAKETGKV
                     DILYLPSMPSINHPVFSDSSFFYETGVTLIVLLVIAIGCGFSINHQLKIINANHQKNK
                     GQQSL"
     gene            complement(5804..6439)
                     /locus_tag="P5123_02485"
     CDS             complement(5804..6439)
                     /locus_tag="P5123_02485"
                     /inference="COORDINATES: protein motif:HMM:NF013247.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class IV"
                     /protein_id="HRX15159.1"
                     /translation="MQCLLSEAIRLDNGILYNMDYHNRRCNSARRELFKACNPIDLSE
                     HINVDQKTSGMYKVRVVYDVEVISVNFEPYHIKPVKSLRVVECPNISYRYKYCDRSII
                     NELMQMRKDCDDIIITVNGIISDASSSNLVFVENETGAYFTPKTCMLRGTTISRLIEE
                     GAVKEELLNITDISRFSEVHLVNAMVDMCQKVVPVRNIKGIAGLYSSDDSR"
     gene            complement(6544..7521)
                     /locus_tag="P5123_02490"
     CDS             complement(6544..7521)
                     /locus_tag="P5123_02490"
                     /EC_number="2.6.1.85"
                     /inference="COORDINATES: protein motif:HMM:NF005486.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminodeoxychorismate synthase component I"
                     /protein_id="HRX15160.1"
                     /translation="MITEKDSYYRCPALFDAINNLAVSRIPFFCLISAFADYGYLFPL
                     DNKEACPIVFSFCGNKISSYDNNAIITNFKPPLYNRYQSAFDKVQQYQYDGCTYLVNL
                     TFTSDITLSGDLYDIYNNSQASYRILFPERFVCFSPECFIKIRDGRIITYPMKGTIDA
                     SIKDAESILINDQKEEAEHITIVDLLRNDLSRVADDVQVDQYRYIQKIMSRNGALYQT
                     SSCISGVIGGDWHGRLGSILQQLLPAGSVTGAPKKKTVQIIQEVEDHERGFYTGIAGF
                     YDGSFFDSAVLIRYIEKTDRGYIYKSGGGVTIYSDADSEYKELINKIYV"
     gene            complement(7539..8849)
                     /locus_tag="P5123_02495"
     CDS             complement(7539..8849)
                     /locus_tag="P5123_02495"
                     /inference="COORDINATES: protein motif:HMM:NF014069.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HRX15161.1"
                     /translation="MFKKSYDNSLLLNEILESMGTIIEFDLEDYETHQHAVRVAEGAV
                     FVAEKMGLDADTIQKLYFAALLHDIGKITVSADLLHKRERLTDDEYSQVKNHTVTGSR
                     IIASLPGMTSLASWVRWHHEWWDGSGYPDGLKEAEIPLPVQILSILDMFDSIQTPRAD
                     RDPRQKEEAVRIITEQRGTHFNPKLVDIVLELIHDSHVSISEKPHQDFAALKKKYVDV
                     PFHDSDKNFWQSSNIESLYYILKLFADVIDSKNEYTRGHSTRVSVLAKILCEKMGMPH
                     EDLLKVEIAGLLHDAGKVTIPIEILNKQDSLTEEEWAIVRAHTTKTFEIIGYSAALRE
                     IAFIAACHHERYDGNGYPMKLAKDEIPFLSHVIAVADTYDAVTSARAYHPQRSAEYAY
                     NIIREVSGRQLHPDVVETLLAIPPKHIEGIVDMHISSYAPVQIS"
     gene            complement(8965..9153)
                     /locus_tag="P5123_02500"
     CDS             complement(8965..9153)
                     /locus_tag="P5123_02500"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRX15162.1"
                     /translation="MSCCGENSSADHECCGGDDQKKCCSESVISPEEQLAELFVYKET
                     LEKELAKVNGLIAQISGE"
     gene            9629..10609
                     /locus_tag="P5123_02505"
     CDS             9629..10609
                     /locus_tag="P5123_02505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007059330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DHHA1 domain-containing protein"
                     /protein_id="HRX15163.1"
                     /translation="MRESHFIEELIDILKKEDTVFIQPHNYPDHDGVASAFGLQSLFK
                     FFDIKSHLVYEGEIQRDSLKQMIDSLDIEIHHISEYNMNEQHKIVVVDGCKGNKNVTD
                     LIGDEIAVIDHHLVKSPEDVPLSDIRPDYGACASIISSYYDLLNIDIPQKSATALIIG
                     IVMDTALLTRGTSEQDVSSYSNLYSKADMRLVNSILRNFIQIKDLHFYKEAINNVQIK
                     KEVAFCHFPNGCNQNLLGILGDFFLALQEIDFVILSANNGGLINFSLRNERDDWNAAA
                     IIQEVLKGIGFGGGHADMAGGIIPDSRNFSEEKIKNLFYSSLFGLNTDNR"
     gene            10615..11829
                     /locus_tag="P5123_02510"
     CDS             10615..11829
                     /locus_tag="P5123_02510"
                     /inference="COORDINATES: protein motif:HMM:NF012422.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LuxR C-terminal-related transcriptional
                     regulator"
                     /protein_id="HRX15164.1"
                     /translation="MVNILAVIAFFCIVILIIQLVYALVKIKGFQKVLFSGIATCLIW
                     WLTCCLIGYGTNELTTVWFCFKASVPSFTFLHCFMLHLVLIFTNSRYNRMILAVIYLP
                     SIIFTYLGITSEFVYGAFAKIGNFWTGSPSGSILTLLFSVQYLSYYIISIALLIMFAR
                     RTQSHRLSKISIILAASLFTTILLFNIEPFLLPLISNYKSLMISPNAAIIWVTGVWYA
                     IIHYKLFSYSANSLLETILQNVEFALFVSNSDGQIIRKNSTAETLTKYNNKPIINLKE
                     LIPVDEFGGNNKKRRCKYLLRQNGKPVLVKLSENPVRDNYGDLTMTAATGLIEGLTLF
                     HEVYNLTKREMEVAACLLNGMTAPQISKKYGTAINTIKSQMSSLYSKTGVNSKIELIR
                     KMEDSSVIQKPS"
     gene            complement(11814..13256)
                     /locus_tag="P5123_02515"
     CDS             complement(11814..13256)
                     /locus_tag="P5123_02515"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRX15165.1"
                     /translation="MRKIAIIISIFLFLFTDVIFAVPRAALYSVQSGSESVNALVLET
                     EKIVRMAIEDLGCMQIVETNELIKAASSIELSDTIPGRYFDISRQAGAQFSILFSIYQ
                     TGALSHGEIAIEAHDNALKKHNLKIRVESRILQNVPYLLLRELLSYYSKFPVYYTSVQ
                     KGSGLFLISAGTYSGIQEGQTYKTVSGEYFTVVTAGRYESYVKSKNTLSSTGELPHFA
                     DVGDLEKWNNGIIADNIDRRYGAAYNYSRRSEPVKQYMESLVVVNPLGNILLPGYGSY
                     LNTGYMGFENPKPSSLNMVYTSGLYLTALLYAPVATKFSGNFFPWIQDSDKSDKVYKR
                     QKFLWAMIPITFTVSYLDHLAIQYQRESVLPPGFSNPLAPYVHSLIIPGGGHFYRGDR
                     IAGWSYYTAQSAALYFAFTDDSKDNQKRALLAMGGLKIVELLHLAFAKSSYEFYNREI
                     YSESTTTFFSSLDSDFEGNPRVSAGFQLGF"
     gene            complement(13253..14713)
                     /locus_tag="P5123_02520"
     CDS             complement(13253..14713)
                     /locus_tag="P5123_02520"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRX15166.1"
                     /translation="MISAIIAGFNIRLAAAPMIVSTGLATDTQRSLIMAEFFETHFNN
                     IVRIGGDFDIVKSDSLMQQLKDSNLLEESALMRFMKTAGIDVVVRGSISDRGNSILIN
                     LYAVSYDVPFNGNIFARYRVVVPLKNVSYSSRELSYCMEEHTGRFMAALYSEYKYPVR
                     VQIRNGALNYNDFKVKKNSYNLFRVLDNTGNIILYEKAGRLNVNRGKLALNNFTEDGN
                     YFILRSFDGDSEFLREFYYGRKTEIVFRPPSLEETLITALATPVISVLSPVASPAAYY
                     ASSDFTGLGLWAVNNAPYIYIGAQGLINRPSVLRDEKKDISHFQKSSFYFGIYYFAAA
                     GASMYADAAGSRAANSAAAYNPVDPYMGSEYTALYFSILGSGGGHFYKGYRSWGYLYF
                     HLDNMLLFTSLYYLLDKETYNGSSYSESASEKKYGYIAAGAALLVRAFEIYHCNSLGF
                     NIKNGKTVQSDFSIVPLVAEDPNGKLIAGFLVEKSL"
     gene            14961..16211
                     /locus_tag="P5123_02525"
     CDS             14961..16211
                     /locus_tag="P5123_02525"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HRX15167.1"
                     /translation="MANDVTVLIVEDDELTSADLQTKLNKLGYMNVIRAADSVSAIST
                     IRQYHPDLVIMDVQLKGNTDGIETASYIFEEFNIPVLFLTACSDTEIQKKARISRPYA
                     YMVKPLDIRILASSIESALYRHSIEQENQALQNLRRAAEQANHSKSLFLANMSHELRT
                     PLNSILGFSKLMKMGYDPDEYHDHLDRIISSGDHLLNLINEILDYMKIHAGKAKLNLQ
                     CINPEPVINECLKSVSYCANEKNIEIKCNIADFPITVFADKRRLKQIVLNLLTNAAKF
                     TPSGGEINIRLYEKESQCCIEVQDNGPGIAAEDQSLIFEQFEQISKDSSCEDTGSGLG
                     LAITRILVELHGGIIEIESSPGKGSLFRVILAALHQIQPVAEQVTSQDDCMFVPLKGT
                     LLVVEDNSGNREFLTKYFEKSGKM"
     gene            16211..16492
                     /locus_tag="P5123_02530"
     CDS             16211..16492
                     /locus_tag="P5123_02530"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HRX15168.1"
                     /translation="MAVKDGYECLELFEDGNTKNIVLILMDLRMRGLSGIDTVQELSL
                     RNIDIPVIAVSAFLDEELEKSSAKSRFTESVQKPINITELNSKIKAIFD"
     gene            17079..18035
                     /locus_tag="P5123_02535"
     CDS             17079..18035
                     /locus_tag="P5123_02535"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRX15169.1"
                     /translation="MKKLLSLLLAMSLLLTFAACDDEEEAPDRGTDPFNYFIDYQSES
                     GWKYFSSTVKNYSSDYKYLKVTTFDNPANSNGTYRDVSKITVKQDGSDDKIFTYNSDG
                     LLTEYKIDRNTTKFTFSDDSSFEFTGGTGSYNDDNMLAALNHTSGDVEEKNAYTYDSN
                     NRLTKQTVSTTDAGYTDEDIYSISYEDDYVKEFTWNRNDNDLEKRTFSFDRETKEPVS
                     CSIKTYTDAGREDSSAKIEYEYGDDGYVSKASLTGTDLSGTDLDQTEEYTWSENGFLD
                     KVVYKDKKAGTTITYDYEYSKGVLLSIKKDGSDYKTYEITER"
     gene            complement(18145..>20307)
                     /locus_tag="P5123_02540"
     CDS             complement(18145..>20307)
                     /locus_tag="P5123_02540"
                     /inference="COORDINATES: protein motif:HMM:NF012245.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein"
                     /protein_id="HRX15170.1"
                     /translation="ETVMFKKMKLGTKLITITGAIIITTALVITVAAVVQSSKALSNE
                     AFNKLDAIHAEKHNELMKYFAEMKNQTLVISQSRDVAESIFALVRYHNEMNIQADQNF
                     DMSGTGAGLTRSYNDIYSEVDTQLAKYVDIFGYYDVYIVCYPHGHVMYSHEKNSDLGT
                     NLKYGEYRESQLSRVWDLARNSDEPVVVDLSPYAPRGGEPAMFSGAPIIVDGNRVGVI
                     VLQVNNEEINELVGDAHGMGKTGEVYCVGQDHLMRSDSKLDPEDTMLKREVKTAAAEK
                     ALNKQEGMEIVKNYHNVNAYTVFSSVGISEVFKTDFDWAIIAEVTEAEILEPVNNLII
                     VISIISLVVVAIAVIITILFSRSISTPLKYGINFAEIVSKGNFTQQMNKEYLRRGDEI
                     GDLARAMDDMSQDLDKTIGNIIYAAQNLVQAVQEISAGNENLSQRTSEQASSLEEIAS
                     TIEQATATINQNADSSRSANKLTQETSVTAGKGGEVIAKAVEAINEVNASSKKIEEII
                     TVIDEIAFQTNLLALNAAVEAARAGEQGRGFAVVAGEVRNLAQRSGNAAKEISTLIKE
                     TVNKVETGTSLANQSGDLIKQIINSIESVTKTVSEIAAASDEQKQGIGQINMAVTELD
                     NMTQQNAGLVEETASASEEMSSQAQELLSMMQKFKINDNGRNNGLSGSGNNIKLSVNR
                     LSEKKVQQTEAVKKDKDGGGKDNKDIEEILSDEGFKQF"
BASE COUNT         6341 a         4306 c         3602 g         6058 t
ORIGIN      
        1 catatggaaa catagaaata ttaccatttc tggaacggct ataccgttta aagccaaatc
       61 atagtattat ttactcacct tatcaatgaa tgaaatctca acacgacgat tacgctggcg
      121 cccctcttcg gtactatttg tatcaatatt ttttgaggta ccatagccat tagtctttat
      181 tcgttcacga ggtataccat tacgttcaag atacttcgca acagattcgg ctcgctcaag
      241 agacaacttg atattaaaat cttccgttcc cacattgtcg gtatgaccat ctatcaccac
      301 tattttatgt ggattatcac gcatatattt tacaagctgt tcaagaaatt tatatgaaga
      361 aggacggata tatgcggaac ccgtatcaaa tcttagatca tgtgccacaa gttttccagt
      421 ctcaggcatt tttacctcag gttctttgat ttctggctct ttaacaggtt cgtatttaat
      481 agcatctttc catagcttat cagctttata gattgtagtt ctgtcagcat tattgtgtgt
      541 tatgaaaaaa ttagactctt ttatatcata atagggtgga gcaacctcgt ttctgtaact
      601 gtttacataa acaacattac gatttattgc tatcgaaggt gttgaatcaa gattcaacac
      661 acccgagtaa atatcaaaac cgcctaatcc cggtcttccg tttgaagaca caaagaccag
      721 atcgctatca cctgtagcaa agacagacgc ctcatctgaa atcgtatttg ccccctcaac
      781 agattttggt ttactccatc ctgatctggt tttttttgaa tagtaaacat caagaccaga
      841 actacctttt ctgttgctaa caaacaaaat ttcagaagat cctttaataa aacaggggta
      901 cgagttaaga tcgccttcat ttagctcttt actttcagtc cagtctttcc aacctctatc
      961 aaaactactt tcttttatta cagccttgct tcctttgcga aaagaggcca aaactgattt
     1021 tccgtttgaa cttatcgaaa tattaattat atcatcatag tcggtatttt ttggcaaagt
     1081 taagcggcca gcggattttg catcaaataa agatctaagc ataactgttt ttaaacgccg
     1141 attgtaagtt attagtgccg cttttgattt atcaacacct aaaacttctt cctcatcaga
     1201 cacaatatca agtcctagaa gctctccttt tgtttctaga gctcttttgt acattcgtcg
     1261 ccggtcattt aatagttttc tttcctcttt actatgcttg tatttattga tgtattttct
     1321 taatgcatta tcagctttat catattgcga tgatgaagca agagcaatta ttcgacaata
     1381 ctctatctcc tgatcactgt ttttcgcaag gtgaatatcg gcttccttta ctgcatcatc
     1441 aaatttttct attgacataa gaaaaagaat aaggttttta ctgtgttcag gaacggaggg
     1501 ctcttttctg atctttttcc gtaatatttc cgtatcagct tcgtaagatt ttatatcatg
     1561 accaggaact tcatatatcc ccgcctctag cgaaaaaaac attaataata ataaaaggcc
     1621 tgttatcctt aaaaacataa ttccatcccc atccagaaga aaaccggaag atcatatacc
     1681 tctctgtgtg aacggttttt ataatcagca atttgatttt ctctatttaa ataaatattc
     1741 catatttctg cataaaatct caaatcatca gaagcatagt agttaacctt agcgtcaagc
     1801 cgatgatgca tgctgtaacg ggcatcattt tccttgccat agagtgtttg tgttcctaac
     1861 ccctctattt cacgtgagcc ggtaacttca gtataaggca aagagctata agcgttaaaa
     1921 agtagagctg gcacccagcg accaaaacgt gcatcagcca ttaaccttag tatatgcgac
     1981 tgattatact ccgaattaat ttctttggcg ttatctccga ataactccac ataacgattg
     2041 tcatcttcaa gatttcttgt ggaagactga aaagtataag ctactgtcgc gttatatcga
     2101 cgcaaaaaac ttctatttgc tcccaattcc agaccgtatg tatagccgtc atcttcaagc
     2161 gcgaatttat gcaattgagc cgcttttcga taagagtcat cattaacagt aattatggaa
     2221 ccgccaggaa aaaggttctg gtgggtagta aagaaaaatt cggcttgcag cctagtttta
     2281 agcaaatcgt aactgattac acctaaagag tatgtttcgg taattgtgtt ttttaattgg
     2341 gcgggtgtaa ccatctctgg attctcggaa tcggcatcaa cagcacccaa ataatagatc
     2401 atgtctggat gagcgatgaa tcgaccggct tttaaataaa cagaagtagt ccgataagcg
     2461 ttaaaaccca attccgcctg atatccaaaa tcactgttat tctgaacttc aaatttatcg
     2521 tagttaactc cggctaaaaa ccagacccta tcaaacctag ccgaaaaatc tgcaaaaaaa
     2581 cgtagaccat aaccatctat agcaaactca gttgattctg tttcagcctc tgaagacagg
     2641 atatcaggtg tagccaagcc ctccatcgaa atattgctga ctactccatt ggaataagag
     2701 gtatactcgt atccaccgga taccgcaaaa gagcccatgc ccgccgataa tgactgataa
     2761 actcggtatt caaccggatc aaaaacaatc gatgcaccgt tttcgacatc tatatcaagc
     2821 tctcggttaa tctgatatcg ggaaagggaa gtttccagat aaaaaatcct gtttattcga
     2881 taatcccatt gaagtgcaaa aaggctgtat ccgctttctg acaatgcgtc atcccattta
     2941 aatgtctcac cagccccatt cgctataaac ctgaagcgat gtgagtcatt caacacataa
     3001 gttgttttca ggtaatagtc ctgaagaaaa taattattgt cctccacatc aagaagaata
     3061 tcgctagtag aacgtcttcc aaccgcgact accgaccatc gaggatcaat cacccccatg
     3121 acagcaacag ttggaagaat gggaagaatc ccgtctagaa taactttgcc ttgtatcccg
     3181 gcttgagacg gttcggcagt ccttatatca ataacattac cggaactgtc tcccattctt
     3241 accggtgcat ctcccttata gagaacaacc tggtctattg ccggatcact aatagccgat
     3301 ataaccggtg cgaatgataa tgcaaagtga tatggattat taatcggaat accatcaata
     3361 tagtatttat ttaaaagagg gtttgtgcta cgaataatgg gaaccgaagc taatgaatat
     3421 ccacctccaa caccgggaat cgtttgaagc agatgcatgc tatcacctat gccacgcata
     3481 ggatatcttg ttatctgttc attactaatg cggtaacgtg tatcaaaaag tggggggact
     3541 tcattaagtt caacaggatc ggcactgttt tgctttttac ttatttcaaa aactgcacga
     3601 aaattactct taactcgtat tccaaaacga tgatgtcccc aagtagcgtt ggacaaatgg
     3661 agataataat ctcctggctc tacattttca aaaacgaatg atccgtctga agctgtttct
     3721 gctgtataaa aattattatc tagcccaaca accaaacccg caacaggacg gccggctgta
     3781 tctgtcactt tacccgaaac cgtacctgcc atcaacgtgc cacatgcaaa aataaaaaaa
     3841 atagcaatca aatatctcat tttattaatt cctaaaaaca actttaagta taaaatcaca
     3901 ctcctggggt tcttctttat gctcaacagg cctaaatttt gaagatctta taaccgattc
     3961 aaggtatccg tccaaaccag cacccgcata tttgacaatt gaataattga taactcgtcc
     4021 atttttatcc actgttacgc gcacaaggga cgccccgatc accggcgaat gcctttcgac
     4081 tggtttaaaa tcaatattcg acaaggtaac aactgcaggc ttattaagct ctatatttaa
     4141 ggagaagtgc tctttcttaa gtttatgcga atagtacaga ttactatatt cataattcaa
     4201 aacttcggta ttgccagaca cattatctcg tgtctgataa aaagatgagc cacatgaaaa
     4261 aaacagcata aaaagcataa aataaaaaat atttttcagt gtcatcatcc gcgtcctctt
     4321 tttaacggtt atatttaaaa ttatagcgct gatacataac agcaagtccg gcataaaaac
     4381 ccgtatgtct aataccgcac tctacccaac catcatcggc ttttgcgtca tgagaaatct
     4441 gatcaattga cccgcgcact attgcaagca ttgtaacatt atacggctca taatgaacac
     4501 caaccccaaa agttgctccc gcggctattg atttgcttcg ttcgaaatct gatgtcatat
     4561 ctgattcaag atcagtaaaa cggtagttac caagataagc attcacatac aaagcgggtt
     4621 caaaatcaaa acgggggaaa aaagtgtaaa agcctgtaag gccaacagta aaaccatgcc
     4681 ctttaagttg cgtgtccaga taatcactcg aatacgaaaa ataacgatat cctatatagg
     4741 gatatgtgaa agatgaattc cccaaatcag aaagtcttaa tcctgctcta acatcaagtg
     4801 tattaatttt ataagaaata gtctcatctc gaaaatattc agaatattct attttctgat
     4861 tatcgaccga agtcgaatag aatctggcat ctgccatcaa taatccatgg aaatatccgg
     4921 cttcaaggga ataatccatt aacgacgtga tatcggactc tgtagaagcg tcaacactgc
     4981 cttttggttc ataatcataa agaacccgat tacctggaga gagcccaatt ggagaaaaac
     5041 tgcccagcac gtaaccgcca cgctgtatat cgcaaaaggc agagaaagag cctaacagga
     5101 taattaaacc cgataaaatc acgaaacgaa gcagatttaa acacatattc tgactcctat
     5161 ttaacagttc tctcataaaa agcgtaaaaa ccattttgtc aatcagatta tgatttatat
     5221 ggtttgcgag cgataaacaa tcagcagtat attattatag acgtaaaagc ctatattatt
     5281 taccttgaca ccataatcag tttttcgaaa atgttcactg ttttttatcc ttgaaaatga
     5341 agaaaattaa tgaataaaaa tataatctca ataatagttg ctatggctat attaattccg
     5401 gctcttatag catcatatat aacatttaca aacattcgag atgaaataag actaaacttg
     5461 tttggtaaaa tttctactgc cgatgtcacg tcacacagaa tgaggcctgt taccgccgac
     5521 aaaatagtgt acgagcttac atacaacttc actatagacg gcaatgaatt ttccatgtca
     5581 aaagacagtg acgaccaaaa ctgggcttcg ttgccaaaga aacagtggga aaacgcaaaa
     5641 gagactggca aggttgatat actctatttg ccatcaatgc cgtcaattaa ccaccccgtc
     5701 ttctcggata gttcattctt ctatgaaact ggcgtaaccc tgattgtttt acttgtaatt
     5761 gcaataggtt gcggtttttc tataaatcac cagttaaaaa ttattaacgc gaatcatcag
     5821 aagaataaag gccagcaatc cctttaatat ttcttacagg aacaaccttt tgacacatgt
     5881 cgaccatagc gttaacgaga tgaacctccg aaaaacgact tatatctgtt atattaagca
     5941 gctcctcttt cacagcacct tcttcaataa gccttgaaat tgtggtcccc cgcagcatgc
     6001 acgtttttgg agtaaaataa gcacctgttt cattttcaac aaaaacaaga ttggatgatg
     6061 aagcgtcact tataataccg ttaactgtaa ttattatatc atcacagtct tttctcattt
     6121 gcattaattc atttattata ctgcgatcac aatatttata acgataagag atgttcggac
     6181 actcaacaac ccttagcgac tttacaggtt ttatatgata tggctcaaaa ttcaccgaaa
     6241 ttacttccac atcgtaaaca acacggactt tatacattcc gcttgttttt tgatcgacat
     6301 taatatgttc actcaagtct attggattac aagctttaaa gagctcacgc ctggctgaat
     6361 tacagcgcct attgtgataa tccatattat aaaggattcc gttatctaac ctaattgctt
     6421 cggaaagaag acattgcatt tcttaactcc gtttcttaat tacttggaac atcgataaat
     6481 ttcgatattc cagagagtaa tattatttta gtgataaggt caattttcgc aagttccttg
     6541 tttttaaaca taaatcttgt taatcaactc tttatattcg gaatcggcat cactatatat
     6601 cgttactcca ccgccacttt tatatatata acccctatca gttttttcaa tatatctaat
     6661 caaaacagcc gaatcaaaaa aactgccatc gtaaaaccct gctatgcccg tataaaaacc
     6721 gcgctcatgg tcttccactt cttgaattat ctgtaccgtt tttttcttgg gggcacctgt
     6781 aacggaacct gccggcagta gttgttgcaa aatagatcct agacggccat gccagtctcc
     6841 ccctatgact ccgctgatac acgagcttgt ctgataaaga gctccatttc ttgacattat
     6901 tttttgtata taacggtatt ggtctacctg aacatcatcc gccactctag aaagatcatt
     6961 tcttagaaga tcgacaattg taatatgctc cgcctcttcc ttttgatcat taatcagaat
     7021 tgattccgca tcttttattg acgcgtctat cgtacctttc atgggatatg taataatcct
     7081 gccatcccta attttaataa agcattcggg agaaaaacag acaaaccttt ccggaaacaa
     7141 tatccgatat gaagcctgtg agttattata tatatcatat aaatcaccac ttagagttat
     7201 gtcagaggta aaagtcagat tcaccagata ggtacagcca tcatattgat attgctgaac
     7261 cttatcaaat gcactttgat atctattata aagtggaggt ttgaagtttg taataattgc
     7321 attattatca taactgctaa ttttgttacc acaaaagcta aaaactatcg gacacgcctc
     7381 tttattatca agtggaaaca gatatccata atcggcaaat gccgaaatta gacagaagaa
     7441 ggggatacgc gatacggcca gattatttat tgcatcaaac agcgccgggc atctataata
     7501 agaatctttt tcagtaatca tattccagaa gataagactc agcttatttg gacaggagca
     7561 tacgacgaaa tatgcatatc cactataccc tctatatgtt ttggaggtat tgcaagaagc
     7621 gtctcgacaa catccggatg aagttgacgg cccgatactt cacgtataat attataggcg
     7681 tactcggcac ttctctgagg atggtaggcg cgcgctgaag tcactgcgtc gtatgtatcg
     7741 gcaacagcta tcacatgtga caaaaagggg atttcatctt ttgcaagctt cattggatat
     7801 ccgttaccat cataccgttc atgatggcag gcagcaataa aggcaatttc ccgtaatgct
     7861 gccgaatatc cgataatctc gaaggttttg gttgtatgag cacgaactat cgcccactcc
     7921 tcttctgtta acgaatcctg tttgttaaga atctctattg gtatggttac cttgcccgcg
     7981 tcatgcaaaa gtcccgcgat ttcgaccttg agaagatctt catgaggcat ccccattttt
     8041 tcacacaata tcttggctaa aaccgaaacc cgggtagaat gaccacgcgt atattcgttt
     8101 tttgaatcta taacatcggc aaatagtttc aaaatataat agagactctc tatatttgaa
     8161 gattgccaga agtttttatc gctatcatga aaaggcacat caacatattt tttctttaag
     8221 gctgcaaagt cctggtgcgg tttttctgat atcgatacat gcgaatcatg aataagctca
     8281 agaacgatgt ctaccaattt aggattaaaa tgagttccgc gctgttcggt aataatacgt
     8341 acagcctcct ctttttgacg gggatccctg tcggcacgag gcgtctgtat cgaatcaaac
     8401 atatcgagta tacttaaaat ctggacaggt aaaggaattt cagcctcttt taacccgtca
     8461 gggtagccac ttccatccca ccattcatga tgccacctga cccaagaggc cagagaagtc
     8521 attccaggaa gtgaagcaat tattcgactt ccagtgacag tgtggttttt aacctggctg
     8581 tactcgtcat cggttaatcg ttctcttttg tgcagcaggt cagcagaaac cgtaatttta
     8641 ccaatatcat gcaaaagagc cgcgaaatag agcttttgta ttgtatccgc gtccagaccc
     8701 atcttttcag ccacaaagac agctccttcc gcgacacgca ccgcatgctg atgagtctcg
     8761 tagtcttcaa ggtcaaactc tataatggtg cccattgatt cgagaatctc attcaaaagc
     8821 agagaattat catagctctt tttaaacata taggcaccct aattgtttat gacccttatt
     8881 ttcaagaatt caaatatata cccgtagtta actttgtcaa gaaaagcctg tataacgtga
     8941 caaaaagctt ttatagacaa ataattattc gccgctgatt tgtgctatca gtccattaac
     9001 ttttgcaagc tccttttcaa gagtctcttt atagacgaaa agctcggcaa gctgctcttc
     9061 agggcttata accgattcag agcaacattt tttttgatca tctccaccgc agcactcatg
     9121 atctgcactg ctgttttctc cacaacacga catattaacc tcccaggtta tttatgtttt
     9181 tctaatactt taaaaatttg atattatact gaattataaa ttttttacca tacttactag
     9241 taatatcaca aaatcaataa tattaaatta ctatttttca ataaaaattt atgataaatg
     9301 atcgtgtgtg gttataaaaa caagatctgt ttactaaaac caaacaataa atggatcttc
     9361 ttgtcattaa ttccaaaatt aataaaaagc tcaaagcgta aaatattatt tagtagacaa
     9421 cactatagtc actatggtat attcagcatg attatttaaa cttaaacttt ttcatgacct
     9481 atatcgtcaa aaatgttgtt acaggttttt tctattacct gttttttatc agagacattc
     9541 aacctatctt gttaagcttt atttccaatc gaattccatt tgcttatgct gactttatat
     9601 ccctgctttt catttttatg gaggcaatat gcgtgaaagt cattttatag aagagctgat
     9661 cgacatcttg aaaaaagagg atactgtctt tattcagcct cacaattacc ctgatcatga
     9721 cggtgtcgcc agcgcctttg gcctgcaaag ccttttcaaa ttttttgata taaaatcaca
     9781 tcttgtatac gaaggggaaa ttcaaagaga ttcgttgaag cagatgatag acagccttga
     9841 tatagagata caccatatca gcgaatataa catgaacgag caacacaaga ttgtcgttgt
     9901 ggacggatgc aaaggaaata aaaatgtcac cgacctgatc ggcgatgaaa ttgctgtgat
     9961 tgaccaccat cttgtaaaat ctcccgaaga tgtaccctta agtgatatca gacccgatta
    10021 tggtgcctgc gcttcgataa taagctcata ctatgatctt cttaatatcg acattccaca
    10081 aaaaagtgcc accgccctta ttataggcat agtaatggac accgctcttc ttacccgagg
    10141 tacatcagaa caggacgtaa gctcatattc caatctttac agtaaggcag atatgaggct
    10201 tgtgaattca attcttagaa actttataca aatcaaggat cttcattttt acaaagaagc
    10261 cataaacaat gttcaaatca aaaaggaggt tgccttttgt cactttccaa acggctgtaa
    10321 tcagaatctt ttggggatat taggtgactt ttttttggca ttacaggaga tcgattttgt
    10381 aatattgagt gcaaacaacg gtggattgat aaatttctcg ctcagaaacg agcgtgatga
    10441 ctggaacgcc gccgcgataa ttcaagaagt tctaaaagga ataggctttg gaggaggtca
    10501 cgcagatatg gcaggcggga ttattcccga cagccgcaat tttagcgagg aaaaaataaa
    10561 aaatttattt tactcttcgc tattcggttt aaatacggat aacaggtaaa actcatggtt
    10621 aatatacttg cggtaattgc gtttttttgc attgtcatac tgattattca gttagtttat
    10681 gcccttgtta aaattaaagg atttcagaaa gttctttttt caggtatagc cacttgcctg
    10741 atctggtggc ttacatgctg cttgattggt tacggcacaa acgaattaac cacagtatgg
    10801 ttctgtttta aagcttcagt accatcattt acttttcttc actgttttat gctgcattta
    10861 gttctcattt ttacaaatag cagatacaac cgaatgattt tggccgttat ttatcttccc
    10921 tcaataatct ttacctattt aggtataact tctgaatttg tttacggtgc gtttgccaaa
    10981 ataggcaatt tctggaccgg tagtccaagc ggaagcattc tgacccttct tttttctgtg
    11041 caatatcttt catattatat aatttccata gcacttctta taatgttcgc aaggagaact
    11101 caaagtcacc gattatcaaa aatttcgatt attcttgctg cttcactttt tacaactatt
    11161 cttcttttta atattgaacc gtttttgtta cctcttatca gcaattacaa aagtctgatg
    11221 atctctccaa acgcagcaat aatctgggtt acaggcgtct ggtatgccat tattcactat
    11281 aaactgttca gctactcggc caactcgttg ctagaaacaa ttcttcagaa cgtcgagttt
    11341 gccctgtttg tgagcaacag tgatggacag attatcagaa aaaacagtac ggcagaaaca
    11401 ctgaccaaat acaataataa accgatcata aatctgaagg aacttatacc tgtggatgaa
    11461 tttggcggta acaacaaaaa aaggcgctgc aaataccttt taagacaaaa cggaaaacct
    11521 gttctggtta agttatccga aaaccctgtt cgcgacaact atggagacct tacaatgacc
    11581 gctgctaccg gattgatcga gggccttacc ctttttcatg aagtttacaa cctgaccaaa
    11641 agagagatgg aggtcgccgc gtgccttctg aacggtatga ccgcgcccca aatttcaaaa
    11701 aaatatggaa ccgcaattaa tacaattaaa agtcagatga gctctctata ctcaaaaacc
    11761 ggagtaaact cgaaaattga actgataaga aaaatggaag actcttcggt tattcaaaaa
    11821 cccagttgaa aaccggcact taccctcggg ttgccttcga aatcggaatc cagagagcta
    11881 aaaaacgtag tggtactttc actatatatc tcgcgattat aaaattcata agaacttttt
    11941 gcaaatgcaa gatgcaataa ttctactatt tttagaccgc ccatagccag caaagccctc
    12001 ttttgattat cctttgaatc atctgtaaaa gcaaaatata gcgccgccga ctgcgcagtg
    12061 taatagctcc atcctgcgat ccggtcgcca cgataaaaat ggccgccccc gggaataata
    12121 agcgaatgaa cataaggagc cagcggattc gaaaaacctg gcggaagcac cgattcacgc
    12181 tgatactgga ttgccagatg atcaagatac gagactgtaa aagttattgg aatcatcgcc
    12241 cataaaaact tctgtcgttt ataaacctta tccgatttat ccgagtcttg tatccatgga
    12301 aaaaaattac cgctaaattt tgttgccacc ggagcatata aaagtgctgt aaggtacaaa
    12361 cctgatgtgt aaaccatatt caaagatgag ggttttggat tttcaaaacc catataacct
    12421 gtatttagat acgatccgta acctggcaag agaatgtttc caaggggatt aactacgacg
    12481 agtgactcca tatactgttt aacaggttca cttcttctag agtaattata agcagccccg
    12541 taacgcctgt cgatattgtc agctattata ccgttgttcc acttttcaag atcgccaaca
    12601 tcggcaaaat ggggcaattc gcctgtagat gaaagagtat tttttgattt aacataagac
    12661 tcatatcgtc cggccgtgac gactgtaaag tattcacccg ataccgtttt gtaggtctgc
    12721 ccttcctgaa ttccactata tgttcccgcg cttattaaaa ataatccgct acccttttga
    12781 acagaggtat agtaaaccgg aaatttagaa tagtacgaaa gcaactctct taaaagaaga
    12841 tacggaacat tttgaagtat gcgactttcg acacgaatct tcagattatg tttttttaag
    12901 gcattatcat gggcttctat ggcaatttcg ccatgagaaa gggctccggt ctgataaatt
    12961 gaaaatagta tagagaactg cgcgccggcc tgacgcgaga tatcgaaata cctaccagga
    13021 atagtatcac ttaactcgat ggaagaagcc gccttgataa gctcgtttgt ttcgacgatc
    13081 tgcatacagc caagatcttc aatagccata cgaactattt tttctgtctc aagaacgagg
    13141 gcattaaccg attcagaccc cgactgtaca gaatagagcg ctgctcgcgg aacagcaaaa
    13201 attacatcgg taaataagaa aagaaaaatt gaaattataa tcgcgatttt cctcataagc
    13261 tcttctccac taaaaacccc gcaatcagct ttccgttagg atcctccgcg acaaggggaa
    13321 caatggaaaa atcactttga actgtttttc catttttaat gttaaacccc aatgaattgc
    13381 agtgatatat ctcaaaagca cgcactagaa gagcagctcc ggctgcgatg tatccatatt
    13441 ttttttctga tgcagattcg gaataggaag aaccgttata agtttcctta tccaaaagat
    13501 aatagagaga ggtaaaaaga agcatattgt ccagatgaaa atagagataa ccccaagagc
    13561 gatatccttt ataaaaatga cctccgccgc ttcccaatat ggaaaaatag agagcagtat
    13621 attcactacc catataggga tcaacaggat tgtacgccgc ggcgctgttt gccgcccggg
    13681 acccggctgc atcagcgtac attgaagcgc cggctgcagc aaaataatag attccaaagt
    13741 aaaaggagct cttttgaaaa tggctaatat cctttttttc atcgcgtaaa accgaaggtc
    13801 tgtttatcaa accttgagcg cctatataga tatagggcgc gttattaact gcccaaagac
    13861 ccaaccccgt aaaatcggag gaagcgtagt aggcggcagg agaggcaact ggcgaaagaa
    13921 cagaaataac tggtgtcgca agagcagtaa taagagtctc ttctaaagat gggggccgga
    13981 atactatttc ggtttttctt ccatagtaga actctcgtaa aaattcggag tctccgtcaa
    14041 atgatcttag aataaagtag tttccgtctt cggtaaaatt atttaacgca agtttgcccc
    14101 gatttacgtt taatcgcccg gctttttcgt aaagaataat attcccagta ttatctaaaa
    14161 cacgaaaaag attataactg tttttcttta ctttaaaatc attatagttc aaagctccgt
    14221 ttcttatctg aacacgaacc ggatatttat actcactgta aagagcagcc ataaatcgcc
    14281 ctgtatgctc ttccatgcag tagcttaatt cacgtgaaga ataagaaaca ttttttaacg
    14341 gaacaacaac ccggtaacgc gcgaagatat taccgttaaa cggaacatcg taagaaacag
    14401 catataaatt gatgagtatt gaattacctc tgtcgcttat agatcctctt acaacaacat
    14461 caatgcctgc cgttttcata aaccgcataa gagccgactc ttccagcaga ttgctatcct
    14521 taagttgctg cattaaagaa tcggacttta ctatatcaaa atctccacca attcgtacaa
    14581 tattattaaa atgtgtctca aaaaattctg ccattattaa agagcgttga gtatcagtcg
    14641 caagaccggt ggaaacaatc atcggagctg cagcaagccg tatattaaaa ccagcaataa
    14701 tagcggagat cattaacagc atcagaattt gttctgtttt ttttggtaaa atcacagtat
    14761 ctcacatccg tttaacatat ttgcaacgca taaaaagctc ttcttaacca cataccatta
    14821 ctgcaacaca agagacaatt cttttttacg ggataaagct gtcacatatt ttgttgtcat
    14881 agctgttgtt attcattata ctttacaatt aagggatttt acggaacaaa accggattta
    14941 acacacaaca ggattggact atggcgaatg atgtaactgt tcttatagtt gaagatgacg
    15001 agttaacatc tgctgatctg cagacaaaac tcaacaaact gggatacatg aacgtaatcc
    15061 gggctgccga ttcggtttcg gccatatcta ccattcggca ataccatccg gatcttgtaa
    15121 taatggatgt tcagcttaaa ggtaacacgg acggaatcga aaccgcttcg tatatttttg
    15181 aagaattcaa tatccctgtt ctctttctta ccgcctgcag cgatacggaa atacaaaaaa
    15241 aagcgcgtat cagccgcccg tacgcctaca tggtaaagcc tcttgacata cggattcttg
    15301 cgtcatccat cgaatcggct ctttaccgtc acagcataga acaggaaaac caggcactac
    15361 agaacctaag acgtgccgca gaacaggcta accactcaaa atcgctcttt cttgccaata
    15421 tgagccacga actgcgaacc cctcttaact cgatactcgg attttcaaaa cttatgaaaa
    15481 tggggtatga ccccgacgag tatcacgatc atcttgacag aatcatttca agcggtgacc
    15541 atctgctaaa tcttatcaac gaaattcttg attatatgaa aattcacgcg ggaaaggcaa
    15601 aacttaatct tcagtgcata aaccccgaac cggttattaa cgagtgcctt aaatctgtca
    15661 gttattgtgc taatgaaaaa aatatagaga tcaaatgcaa tatagccgac tttccgataa
    15721 ccgtttttgc cgacaaacga agattaaagc agatagtact taatttgcta accaatgccg
    15781 cgaagtttac cccatcgggc ggagagataa atatcaggct ctatgaaaaa gagagccaat
    15841 gttgtataga agttcaagat aacgggcccg gcattgctgc tgaagatcaa tcgcttatat
    15901 ttgaacagtt cgaacagatt tccaaagatt catcctgcga agatacggga agcggtttag
    15961 gtcttgccat aacacggata ctggtcgaat tacacggtgg aatcatagag attgagagca
    16021 gcccaggcaa gggttctctt ttcagggtga ttcttgcagc gctacaccag attcagccag
    16081 ttgcagaaca ggttacgtct caggacgact gcatgtttgt accgttaaaa ggtactttgc
    16141 ttgttgtaga ggacaacagt ggcaacaggg aattcttgac aaaatacttc gaaaaatcgg
    16201 gcaaaatgta attgcagtaa aagacggcta tgaatgtctt gagctttttg aagacggaaa
    16261 cacaaaaaac attgtactga ttttaatgga tctgagaatg agggggttga gcggaattga
    16321 caccgtacaa gagcttagtc ttcgcaatat tgacattcca gtcatcgctg taagcgcgtt
    16381 tctggatgag gagcttgaaa aaagtagcgc aaaaagcaga ttcactgaat cggtacaaaa
    16441 acctatcaat attaccgagc ttaacagcaa gattaaggcg atttttgatt aatctttgct
    16501 taatatctta tccggacatt cagaattttt cacacctttg cctggttaat aaaacatcgg
    16561 cagcctctaa aaaattaaca ttaccaaccg aaacttacat aaaatttctt taaaacagtt
    16621 tttttgagcg gcgccatttt aaaaaaaact gtaatttttc gaatttctct attgttaatt
    16681 tctcacaact gcactgcaag cagttgcaca attcgtgttt ttcctgaaaa aaaactttgc
    16741 caactttttt ctgtaactca ctgtcttttt tttcacctga attaaccata ccgttttaag
    16801 atatgaacct gcttgatagt gaaaatccat gagccgccat atcatggaag gcaaatattt
    16861 atcaaaaaag agttgaatta attaagtatt tttgtatctc ataaacattg tgagttttta
    16921 agccctattt gcggttaaca ttaattcgtt tagaatagtt cctaaacgaa ttcaatagga
    16981 attttgctaa actctttaat gcagtttata aaaccatttt ccgcaaaaca aaataacttt
    17041 taagtaacac tatttttgac aaacaaggag aattaaaaat gaaaaaacta ttatcactac
    17101 ttttggctat gtcgcttctt ttgacctttg ctgcctgcga cgacgaagaa gaggctccgg
    17161 acagaggtac agatcctttt aactatttta ttgattacca gtcagaatcc ggttggaaat
    17221 atttctcttc aacggtgaaa aattactcat ctgattacaa atacctgaaa gtcacaactt
    17281 ttgataatcc tgcgaattct aatggcactt atcgggacgt ttccaaaatt actgtaaaac
    17341 aggatggttc tgacgataag atctttactt ataatagtga tggactgctg acagagtaca
    17401 agattgacag aaatactaca aaattcacat tcagtgatga ttccagtttt gagtttaccg
    17461 gtggaaccgg cagttataac gacgacaaca tgctggctgc ccttaaccat acttcaggag
    17521 acgttgaaga aaaaaatgcg tacacctacg atagcaataa cagattaaca aaacaaaccg
    17581 tttctactac agatgccggt tatacagatg aagatatcta ctctataagc tatgaagatg
    17641 attatgtaaa ggagttcacc tggaatcgaa atgataatga tctggaaaag agaacattct
    17701 ctttcgatag agaaaccaaa gaacctgtgt cttgttcaat aaaaacttat acagacgcgg
    17761 gaagagaaga ttccagtgca aaaattgagt acgaatatgg tgacgacggt tatgtctcaa
    17821 aagcatctct gacaggaaca gacttgagcg gaactgacct tgatcagact gaagagtaca
    17881 catggagcga aaacggtttt cttgacaaag tcgtttacaa agacaagaaa gccggcacaa
    17941 ccattactta tgattatgaa tacagcaaag gtgttcttct aagcatcaaa aaagatggaa
    18001 gcgactataa gacttatgag attactgaaa gataattttt tgaattttta tcaaattttg
    18061 taaaacatga ggcgccgctt gcagcggcgc ttttttaatg gcaatattat taactacggc
    18121 aaaacagtcc ttttgcacat cgtcttaaaa ctgcttaaaa ccctcatccg aaagaatctc
    18181 ttctatatct ttattatctt taccgccgcc atctttatcc tttttaacag cctctgtttg
    18241 ctgaaccttt ttttcgctta accggtttac ggaaagctta atgttattac cggaaccgct
    18301 taagccgtta tttcggccgt tatcattaat tttgaatttc tgcatcattg agagaagctc
    18361 ctgtgcctgg cttgacatct cttctgatgc cgaggcagtc tcttcaacaa gaccggcatt
    18421 ttgctgtgtc atattatcca gctcggtaac ggccatatta atctgcccga tcccctgttt
    18481 ctgctcatca gaagccgcag caatttcgga tacggtcttt gttaccgact ctattgaatt
    18541 tattatctgt ttaataagat caccactctg gttcgcaaga gacgttccgg tctcgacctt
    18601 gtttactgtc tctttaatca gggtacttat ctcttttgcc gcattaccag atcgctgagc
    18661 caggttacga acctcacccg cgacaacagc aaaaccacgc ccctgctcac cggcacgcgc
    18721 ggcctcgact gcggcattaa gagccaaaag atttgtctga aacgcgatct cgtcaattac
    18781 tgtgataatc tcttcaattt ttttactgga cgcgttaacc tcgttaattg cttcaaccgc
    18841 tttggcaatc acttcgccgc ctttcccagc agttaccgaa gtctcttgtg ttaacttgtt
    18901 cgcgcttcta gagctatcgg cgttttgatt aatggttgca gttgcctgtt caattgtcga
    18961 tgctatctct tcgagcgatg atgcctgttc ggaagtacgc tgtgacaggt tttcattacc
    19021 ggccgatatt tcttgtacgg cctgtacaag attttgcgct gcataaataa tattgcctat
    19081 agttttatcc agatcttgcg acatatcatc cattgcgcga gcaaggtcgc ctatctcgtc
    19141 accgcgtctc aagtactctt tattcatctg ttgcgtaaaa tttcccttag atactatttc
    19201 cgcaaagtta atgccatatt ttaaaggtgt ggatattgat cgtgaaaaca aaattgtaat
    19261 tattacagca atagctacca caacgagcga aattattgaa ataacaataa tcaaattatt
    19321 tacaggttca agaatctctg cttcggttac ctcggcgata attgcccaat caaaatcggt
    19381 cttaaagact tccgaaattc caacagaaga gaagacagta taggcgttca cattgtgata
    19441 gtttttaaca atttccattc cctcctgttt attcagagcc ttttcggcag ctgctgtttt
    19501 aacctcccgt ttcagcattg tatcttccgg atccagtttt gaatcactac gcatcagatg
    19561 atcctgcccc acgcagtaaa cctcaccggt tttgcccatc ccatgggcgt ctccaaccag
    19621 ttcgtttatc tcttcattat taacctgcag aacaattact ccgaccctat tgccatctac
    19681 aattattggc gcgccactaa acatagcggg ttctccacca cgaggagcgt aaggagacag
    19741 gtccacaact accggctcat cgctattacg agccagatcc cagacccttg acaattgcga
    19801 ctccctgtat tcaccatatt ttaaatttgt accaagatca ctgttttttt catggctgta
    19861 cattacgtga ccatgtggat aacatacgat ataaacatcg tagtatccaa atatatctac
    19921 atattttgcc aactgggtat caacttcact ataaatatca ttatacgaac gggttaaccc
    19981 agcccctgtt cccgacatat caaaattctg atctgcctga atattcatct cgttgtgata
    20041 gcgcacaagc gcaaaaattg actcggcaac atcacgactt tgtgagataa ccagtgtctg
    20101 gtttttcatc tcggcaaaat atttcattaa ttcgttatgt ttttccgcgt gaattgcatc
    20161 caatttgtta aaagcttcgt tggatagagc ttttgatgat tgcacaacag cagccacggt
    20221 tataaccaga gccgttgtta ttattatcgc accagtaatt gttatcagtt tggttccaag
    20281 cttcattttt ttaaacataa cagtctc
//
LOCUS       DAOTGZ010000019        20074 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_3064
            NODE_3241_length_20074_cov_4.812528, whole genome shotgun sequence.
ACCESSION   DAOTGZ010000019 DAOTGZ010000000
VERSION     DAOTGZ010000019.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868088
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 20074)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 20074)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:22:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,785
            CDSs (total)                      :: 2,751
            Genes (coding)                    :: 2,732
            CDSs (with protein)               :: 2,732
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 12 of 19
            Pseudo Genes (internal stop)      :: 5 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20074
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3241_length_20074_cov_4.812528"
                     /isolate="AMR_MDS_3064"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..192
                     /locus_tag="P5123_02545"
     CDS             <1..192
                     /locus_tag="P5123_02545"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HRX15171.1"
                     /translation="KHTIIEENRVREVYNWLKFFTLQTIKDEVALSGFSVVDYYSDVS
                     GAPFDEESETMALVLKKNN"
     gene            complement(244..578)
                     /locus_tag="P5123_02550"
                     /pseudo
     CDS             complement(244..578)
                     /locus_tag="P5123_02550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010869618.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF86 domain-containing protein"
     gene            complement(571..867)
                     /locus_tag="P5123_02555"
     CDS             complement(571..867)
                     /locus_tag="P5123_02555"
                     /inference="COORDINATES: protein motif:HMM:NF014021.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase family protein"
                     /protein_id="HRX15172.1"
                     /translation="MHSSEYILKLLRKEKVRLRSLGVTDIGLFGSYLRGEAKKKSDID
                     ILINISEDSPLTLFSLIELEQNLSEKLNKQVDLVIKRDLKPGIGETILNEVKYA"
     gene            1091..3364
                     /locus_tag="P5123_02560"
     CDS             1091..3364
                     /locus_tag="P5123_02560"
                     /inference="COORDINATES: protein motif:HMM:TIGR01056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003916 - DNA topoisomerase activity
                     [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase 3"
                     /protein_id="HRX15173.1"
                     /translation="MIVCIAEKPSVAREIATVLGARSRHDGYLEGNGYCVTWTYGHLC
                     ELKTPDEYDPKWKRWSLDMLPIIPAKHQTKLIKRKGISKQFSVIKNLLKNCSYVINCG
                     DAGLEGELIQRWVLQQAGYKKEVRRLWISSLTEEAIREGFTSLKSSDEYDSLYYAALS
                     RSVGDWLLGMNASRLYTLKFSGGAGVLSIGRVQTPTLALIVQRHLEIQNFVPEPYYLL
                     KTIYRDTHFTCDKKKISEQYEAQRLCKKTENEPFKITDVSEKKVTEQPPLLYDLTSLQ
                     VDCNKRFGLSADKTLQTVQSLYEKKMVSYPRVDTRYLPDDLYPKVPSILKGLNNYNEY
                     TSRLDMSSLSHSKRIFNNVKVTDHHAIIPTGKSGALAPGIELKIYDLICRTFIAAFYP
                     PAILHKTKVIGETAGIIFIAQGSRVEESGYRILFENDTFKEKGKNEEEPLPLFTVGES
                     GPHNPYVDSKMTKPPAAYTEATLLRAMESAGKQVDDELLRELMKENGLGRPSTRANII
                     ETLFRRNYIRRDKKNIIPTQTGIELIGIIENPLVKSAELTGIWEGKLKQIERGEFPAA
                     EFITSMEEMIVSLIAEVKKQSGTVAIEQKKKKTYAKKSVKKTAGASIALKSKSTVKKQ
                     SVIIEGLACPCCKIGKIVRGRQAYGCNRYKEGCAFVVPFEIRGIKITDKHFHDLLTKG
                     KTSKIKGLKGKNGNVDVSLFIRNNHLEYHGSKENFSDGIAPDMPCPLCGIGKLVKGRF
                     AWGCSRYNDGCRYLIPF"
     gene            3632..5332
                     /locus_tag="P5123_02565"
     CDS             3632..5332
                     /locus_tag="P5123_02565"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRX15174.1"
                     /translation="MKNDLFIKYSDFGKKASIKAIDRALRKANWKNFKASRDISPLRS
                     VLSKIEDKKGVTSLHFFCTSKIENMGARLAYRTPEYRSKNRYTSVRCDGAYAAFFFFV
                     DKECDISIIEMLSGLEVNRLTLRAESDNFDARWSPDGHRIAFFIDDKTFCLLDPFASE
                     VGIKYCTIESACSKSGWCFSPDSTFAAFSVNDRYNGCPLTRVELKNMKIILYNDGFSI
                     PESLSQRINSLDLVLSEKLRWNKELGIIYGTSGLLGVVASFDENSCELVSYSNYRVGI
                     VWSSRSNYLAHLGPCVAMTDSDKNTEIFQNSIVNNAPIAVFTSDKRRLALVMQKNNLS
                     SDILIYDGNFRTGVVETSILNSDQISAGAVLCWSHCSGYMAVLTGKNRLQIWNVSANP
                     YLSSTTEIDNGVRAVIAVCKNNFLVVGDSWLAVVDSKDGSVRCKTDFKLKSKHMLHLD
                     PMKKIHHFVATEGSVVNDFANIYENFSSDALQVFHRLRVGNIICSQQTRFLLKEELCY
                     ILHNRYTWPFHWGAGDRVQADVRFDSILVGNEDLMAQRHIIEIDRKNVSFCMTNNRLH
                     "
     gene            5458..6618
                     /locus_tag="P5123_02570"
     CDS             5458..6618
                     /locus_tag="P5123_02570"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: protein motif:HMM:NF012492.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HRX15175.1"
                     /translation="MNLIDLDIDQRLVDALNKDNIIEPTVIQTMAIGAVFSKKSLIIE
                     SATGTGKTFAYLLPIMSQIDTVTDNLQVLILAPTHELVMQIYELIKCTANEANMPIRS
                     AGFIGKVHINRQVEKLKVKPHVIVGTPGRILDLFKIKKLKLHNVSTVVIDEASSLMLS
                     ESRHAVKTISASLMRDTQRIFVSALFSDQAKKRVLEICPKVEHIRAAEKTNSDIQHCY
                     IEITDSREKFKTLRKFLAAFDKGKTLIFVKKNPDVAIFANKLEFHKVAVLQLSGEMSK
                     IDRQKNLAQFENKKDGVLIATAVAARGLDIDSVDRVVHYDIPIESSDYLHRSGRTGRA
                     GRKGISVAIADSREARRLLRITEEAEISTKRMKMSNGKFCEYIIENNKLEQK"
     gene            6615..6929
                     /locus_tag="P5123_02575"
     CDS             6615..6929
                     /locus_tag="P5123_02575"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRX15176.1"
                     /translation="MRKVIFVAMFIIFIVGYTLVYSEDERGSCEIYADIVCDICGEDH
                     TSCIQMRDKAVEIKEHSLCAKAVKHLPSAYKNASDLSRSLLKKAVCDKKMPETTKAPG
                     QP"
     gene            complement(6926..7138)
                     /locus_tag="P5123_02580"
     CDS             complement(6926..7138)
                     /locus_tag="P5123_02580"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRX15177.1"
                     /translation="MKSNNILNIRLFNKVILFVIESTLGILYLGNKLHGFIGIILGLA
                     VLALFIFNKTGFFPPYKLHNRKRIKH"
     gene            7349..8368
                     /locus_tag="P5123_02585"
     CDS             7349..8368
                     /locus_tag="P5123_02585"
                     /inference="COORDINATES: protein motif:HMM:NF015951.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF368 domain-containing protein"
                     /protein_id="HRX15178.1"
                     /translation="MSYVFNILKGGVVGLANLIPGVSGGTMALILGIYERMIAGLNSI
                     SLDLFLSFFKLLTFKKKNWELFLIELEKIDFKFLVSVFGGALLAILAFASIMVYSLNN
                     YHDQTYGFFWGLVLPSVWVPFKLIKKITPAVSIAFVCATLLVVSTDFLMSDNEQIQKE
                     QQKYEIAIEKTQSGSIMDEFDINPLSLFLLFVAGAVSVSAMILPGISGSFVLLLMGQY
                     FIVLKAVSSFNIVYLAVFSVGLVFGLLSFTKLLHYLLKKFHDITMGALTGLVFGSLWV
                     IWPFKTSYLVGEVGYADGYPQLVYLSNVIPEKLTLVELSSLLSVLAGITVVLVMIKIE
                     KANNQ"
     gene            complement(8513..9355)
                     /locus_tag="P5123_02590"
     CDS             complement(8513..9355)
                     /locus_tag="P5123_02590"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRX15179.1"
                     /translation="MKKVLVALIVLLFLVSCGQNVVITTKAKGNILTNVELKYKNVDS
                     TRPVELKNGERVILDADNFQTFYVDLSGTSDADHIQLISEMEQGLKVMLVFADSKTEK
                     ALAKVEVPKEGIEGYRQEDVVTYVYDRRGKQVGEKRVRKGEKVPFGDTKYTDTSLIPI
                     DADLTSLASRENDVTVYVELDYSSYEMDPAKNTREYTSDELSKKDYKLDVVHNINYRE
                     VYFTTPRRSKMIKATVIKPAAKAETSDDLDMDSEDDFLSDTEDKPKPKDATTSDDFDL
                     DIDI"
     gene            9813..12005
                     /locus_tag="P5123_02595"
     CDS             9813..12005
                     /locus_tag="P5123_02595"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRX15180.1"
                     /translation="MMILHAATVFICLSIVMLFRRADRANTRIIKLKRFTDNSFKEFK
                     KLTDSENRRYNDSTIEMDLLLKKAHSISGSIRTSIDDLEKRLHGLNSEKLGLKKVEDD
                     LRVVSAAADQVNYQIKYIESAKADLGSVVKKINALNENLITIEKNNSTLYQSFNDSLR
                     TRSRELSEELAQEVKKLKASVGDKESVIIAQAQDKIDILGKSFEQSINNMEKGITTAG
                     DAIMQNIRQKLDVLTRTVNNIEETVNTSEKRVYSDLTEAIENAEHEINQIFEKIQNVE
                     ISIDQSRTKLIASFEEETNKIRNSMDSLSLHSIAKKDEIIMAARQEADEIKKIVDKFN
                     EQYNRFEVNLNAVAQEQMETIVHEYESIESNFDKFTQKLEMLDEKIAERQRMQAEKNR
                     EEFSYMEERLAEIKNEIVTYEKSNKVFERTDEMMSRVEGALEEYNAVFEKVQSESSVI
                     EGFVKDLDQFKEMKRELEKELKVYNAKREKLQNVATDIQALFDIAEDVSSKSEFLEDN
                     AVNIDKVNRRIDDLNNTYQALGVKIKELQDFEKNIEKNVSNIDRIEATVNSIDKRVQL
                     FTVSVEKSENKINKLKEYLTSIEEATYVLKTKEHEINHLKERFEEVDMLAAFVDKRLD
                     QLKIAMNRIESMRSDIDSTDSRLQEMFKETDKRMQQFSEFLQTVEPDNLITKQMRGDF
                     VPGSKSINDGMVRTVRELSNKGWTAIDISKKLLLDENSVRLIINTAAQ"
     gene            complement(11999..12772)
                     /locus_tag="P5123_02600"
     CDS             complement(11999..12772)
                     /locus_tag="P5123_02600"
                     /inference="COORDINATES: protein motif:HMM:NF013672.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HRX15181.1"
                     /translation="MARLIGFLICLIILQYSCSSLNSIIVSKENRYKSLPPGRLVCPE
                     SRLKTNKKVIALTFDACGGRGACGYDKEIIDYLKNEKIKATLFLNIRWIKKNRSAFDD
                     LVSNPLFQIENHGLKHKPASINGASMYGVRGTSNIRELVEEVEMSARIFKEYGAKKPK
                     YFRSGTAWYDETSIRIIESMGYKILNFDTNSHDFDKTKTATEISEKILKAKEGSIVIM
                     HMNHPRRNTAEALKLAVPQLREMGYKFVKINEYRVQSIY"
     gene            complement(12781..14658)
                     /locus_tag="P5123_02605"
     CDS             complement(12781..14658)
                     /locus_tag="P5123_02605"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HRX15182.1"
                     /translation="MRNTTVLIVEDNPIEAKDIAAAISKAGYPDSIVCSSGEEAIQYC
                     ESNTPLFILMDIGLSGEIDGIETAEKIIKEHNIPVIYLTASSDDETIDRTKDTYPYGF
                     LLKPVSNRFMHITIEMALNRYDLESQLVKNRNLVNLTLNSILEAVIAVEQGGTIIYIN
                     DQALKIIGLDKKEALKKNIKDIYLTTDPQSGALLDCTPMTVMETGESVIYRESRELIS
                     KNNHKTPISENCSPLINSRHQLFGAVLVFQDISLKKQSDNEKLKNRKMESMSKLARGL
                     AHDFNNILTGVIGNVSMLRMIMETTEPPVQEIINEIEKSSNRARDLANQIRLFAKGSA
                     PIKKAADLNSIITDTGTFILSGSDLKLNISTNANTDHLKFDPDQMSQVFENMFLNVKE
                     ASQGPNTVNVIITKEIIGSTSAYNLQSGNYIRIDINDFGKGITTESADQIFDPFFTTK
                     SKAGGMGLSNAYNTVKAHGGTIVLKSLQSPTSFSIFLPIEIQTKLQNKNIIPIGSHII
                     IVDDEKSILIMLEKMLKALHYTPHLFTHPKTALDYINANRDLCPKIKTFILDLSIPGS
                     EGAVSLLKSLKPEFPDATYILISGHQTENPVIADYQKFGFNALIQKPFSFDDLKILLN
                     R"
     gene            complement(14748..16271)
                     /locus_tag="P5123_02610"
     CDS             complement(14748..16271)
                     /locus_tag="P5123_02610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018250086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YifB family Mg chelatase-like AAA ATPase"
                     /protein_id="HRX15183.1"
                     /translation="MLSKTISLGINGIDAHLVEVEVDTVRGLPGFTIVGLPDSSVKEA
                     KDRIRSAIENSGFLFPPKNYIVNLAPAEFKKQGSNFDLPIAIAILVHSGQTDIHTTPY
                     PMVGELSLDGTLRAVQSTISMTLTLHKKNFDHFIVPYDNRYEANAIDNIKIYPAKSLN
                     EVIKILTTNTKPFTDTRQETQKYFSIDFSTIAGQEGIKRAAEVATAGYHNFLMYGPPG
                     SGKSMIAKAIQGILPKLTKNQAIETTIIHSNAGVLPINSGLITTPPFRSPHHTSSHIS
                     IVGGGRIASPGEISLSHNGILFLDELTEFQGFLLQTMRQPLEDGVITISRAAGTSVYP
                     AKFMLVGACNPCRCGYIFDPEIECTCTNAMIRSYYSKISGPIIDRIDIEHYVPRIAYE
                     KLTNNSGTETSATIRERITECRLIQLERFKNRNYSFNGQMDTEDVRKYCQLSNEDENF
                     LINSSQKMRLSARSYFRILRVARTIADLDKKEGIKKNHILEALSYKNLPRNYEMFLS"
     gene            complement(16357..17889)
                     /gene="dacB"
                     /locus_tag="P5123_02615"
     CDS             complement(16357..17889)
                     /gene="dacB"
                     /locus_tag="P5123_02615"
                     /EC_number="3.4.16.4"
                     /inference="COORDINATES: protein motif:HMM:NF014200.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004175 - endopeptidase activity [Evidence
                     IEA];
                     GO_function: GO:0009002 - serine-type D-Ala-D-Ala
                     carboxypeptidase activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine
                     carboxypeptidase/D-alanyl-D-alanine-endopeptidase"
                     /protein_id="HRX15184.1"
                     /translation="MTNFLKHYLLLLILQFFLSCNINFENRNHTFANEIENSFKPSQV
                     VIGNDTLPKNSVPDFNHFSPSNTGFIVYDTQQKKVLFSHNRKKLFMPASTAKIITATT
                     AFMVLGDNYRYNTSVYYDGEITDGVINGNIYLRGEGDPSFTASDMVNMISKLKKKGVT
                     TITGNFYYDNLSLTSIPIINPLFDEDEAYNSGISALSCEYNYISVNWTRPDKDGNILF
                     NVTPDLPYLKIERGEKELQKHEKFSCISDKYQTIWHLCKNINEFGSHRLPVRNTALHT
                     SLLFQKIAAIHGINLPYPQQNNTPKSSKLLVQHKGETVSSIIKTTLEFSVNQSAELLF
                     LSSASKIVGNKLNYSESSKTILNYIKSKFTKVDWEGFTITNGSGLSTTSRTSPEQLLA
                     FLIYADSKSYSGRELEYYMLPSGWGWSLKRRFNNPDNEFTLYAKTGTIHYANALAGIF
                     YTKSGKRLLFVFLNSDNYRRKVFDKDTEKRGEKAQKEAKKWHNAYLRAMDATIDNWIR
                     TL"
     gene            17980..18432
                     /locus_tag="P5123_02620"
     CDS             17980..18432
                     /locus_tag="P5123_02620"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRX15185.1"
                     /translation="MIDTTICIKPELLSKLEKFALENGMKTVDYALLLLNKLVNSKIY
                     QDLSKKKAVAYQPRCTDGILLKMHIYPDDNFYEILLDSRKIFKCSVSYLLAFAIEEHD
                     CLVDSEKDSKNYTILKVIVCNIPVLIISWGDVSCHKDIVKAIENFEDG"
     gene            18443..18973
                     /locus_tag="P5123_02625"
     CDS             18443..18973
                     /locus_tag="P5123_02625"
                     /inference="COORDINATES: protein motif:HMM:NF024824.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HRX15186.1"
                     /translation="MRTKILTTVMTIIVIIFCAIIYLTVNKGEDISDTAAINFSSKML
                     RQGEVEVALSILNKKIKAGTDNPLIFYNAGVVLLNKREYSKAVELFSESIRLDPSVSA
                     LNNRAFCYTTLNESKKAIDDYTAILEIDENQAASYLRRGQLYFNIGDFKSAKVDLEKA
                     SSLGLKEAKKLLKKME"
     gene            complement(19002..>20074)
                     /locus_tag="P5123_02630"
     CDS             complement(19002..>20074)
                     /locus_tag="P5123_02630"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRX15187.1"
                     /translation="IVIDGVSSDCGEHISMALNGTNLYISYYDSTHDMVKLAHSNDSG
                     TTWEVYLVDKTTCGGTSVAVSGATVHLAYYAENSYASLFSIDIEAAAPITGFNLGGST
                     HDFTLTIDGVPNNITLPDNANTQIDNIVTAINNAYNPDIVAYNYNGRRVAVFEQTISD
                     GNGIGFTNGIATSVDGILFTVPTTVANGDASIQELRYAKSTSLHTWDIQSIESAGYGL
                     APSINADGSNLGVTYVTNDATGTIRYAYSSNNGANWTSYGDLFTPGSTSGDYFGALRF
                     DGSGSALFAFYDEDSQVLMFTKGDYNGTDLDAAQIVIDNNTGRGTFPAMAVDGSDVFI
                     IYFDSVDEDLMIIKSEDLGSSW"
BASE COUNT         6307 a         3217 c         3909 g         6641 t
ORIGIN      
        1 aaacatacaa ttatagaaga aaatcgtgta agagaagttt ataactggtt aaagtttttt
       61 acattacaaa caataaaaga tgaggtcgca ttaagtgggt tttctgttgt tgattattac
      121 tctgatgtga gtggagctcc ttttgatgaa gagtctgaaa caatggctct ggttcttaaa
      181 aaaaataatt agttggatta agttatgcca tgacatataa attatatttt agttgtatca
      241 gatttaatct acaaagttat ttaatttatt gatcagctga gggatatctt tttgtattgt
      301 tttccataat attgatgtat cgacaatgtc atatccgtgt attaggcggt cacgcatgcc
      361 agcgagtatt ttccatggaa tatcagggtt ttttctctga tttctactgg tattcttttt
      421 gcagcttctc ctatgatttc aaaacaacgt attacggcgt atagagtctt attatcgttg
      481 ctaaaggtta taaaatccaa gccttttgta aaatcacaga tgtaatccac ggatattttg
      541 atgtcatcaa tgtaatcaga gtatatccgt ttatgcatac tttacctcat ttaaaattgt
      601 ttcaccaata ccaggtttga gatctctctt gataactaaa tcaacttgtt tgttaagttt
      661 ttctgataaa ttttgctcta attctatcag cgaaaacagt gtaagtggag aatcttcaga
      721 aatatttata aggatatcaa tgtcactttt ctttttggct tcaccacgta aatatgatcc
      781 aaatagaccg atgtctgtca ctccgaggga gcgtaatctt actttttctt ttcgaagaag
      841 tttcagtatg tattctgatg aatgcatttt tactatatcc tatttaaaat ataatcaatc
      901 tttatggtaa gaaaataaat acggcaatca tttttttaaa caatggctct ggttcttaaa
      961 aaaaataatt agttgtatta agttatgatt tacgttgcaa atcatctgtc cgtttaaatt
     1021 cattatgatt gacttataca ggtaaataca gtagttatgc catggcagct gtttgcttgg
     1081 aggtcgatga ttgattgtct gtattgctga aaaacctagt gtagcacgtg aaatagcaac
     1141 agttttgggt gctcgctcaa gacatgacgg gtatctcgag ggtaacggtt actgtgttac
     1201 ctggacatac gggcatcttt gtgagctcaa aacacctgat gagtacgacc caaaatggaa
     1261 aagatggtct cttgatatgc ttccaattat ccctgctaaa caccaaacaa aactgattaa
     1321 gcgcaagggt atatcaaagc aattttcggt tataaagaat ttgttgaaaa attgctcgta
     1381 tgtaataaac tgcggtgatg ctgggctcga aggtgagctg attcagcgtt gggttcttca
     1441 acaggctggt tacaaaaaag aggtccgtcg tttatggata tcatctttaa ccgaagaagc
     1501 catacgtgaa ggatttactt ctcttaaaag ctctgatgaa tacgattcac tttattatgc
     1561 tgctttatcc agatcagtag gcgattggtt gctcggcatg aatgccagcc gcctttatac
     1621 tttaaaattt tccggtggag caggggttct ttcaataggt cgcgtacaga ctccgactct
     1681 agctcttatt gttcaacgtc atctcgagat acaaaatttt gtcccagagc cctattatct
     1741 tttaaaaacc atataccgtg atacccattt tacctgtgac aaaaaaaaga taagtgaaca
     1801 atatgaagca cagagattat gtaaaaaaac tgaaaacgaa ccatttaaaa tcaccgatgt
     1861 ctcagaaaaa aaagttaccg agcaaccccc tcttttgtat gatcttacat ctttgcaagt
     1921 tgactgtaac aaaagatttg gtttgtcagc cgacaaaaca ctccagacgg ttcagagcct
     1981 ttatgaaaaa aaaatggtta gttatcccag agtcgatacg cgttatctgc cagatgattt
     2041 gtatcctaaa gtcccatcaa tactcaaagg gctaaataat tataatgagt atacatcgcg
     2101 tcttgatatg tcatctcttt cgcattcaaa aagaattttc aacaatgtaa aagttactga
     2161 tcaccatgca attatcccaa caggcaagag tggcgccttg gctccgggta tagaattaaa
     2221 gatatacgat cttatttgtc gaacttttat tgcagctttt tatcctcctg caatattgca
     2281 taaaacaaaa gttatcggag aaactgcagg tataatattt atcgctcagg ggagtcgcgt
     2341 agaagagtcg ggatatcgta ttcttttcga aaacgatacg ttcaaggaaa aagggaaaaa
     2401 cgaagaagag ccacttccac tttttaccgt tggagaaagt ggtccacata atccatatgt
     2461 cgattctaag atgacaaagc ctcctgcagc atataccgaa gcaacattgt taagggcgat
     2521 ggagtctgca ggtaagcaag tcgacgatga gttgcttagg gaattaatga aggaaaatgg
     2581 actagggcgc ccgtctactc gtgcaaatat tatagaaaca ctttttcgaa gaaactatat
     2641 aaggcgtgat aaaaaaaata ttattccgac acagaccgga atcgagttaa tcggaataat
     2701 cgaaaatcct ttggtaaaat cagctgagct aaccggtatt tgggagggta agcttaaaca
     2761 gatcgaaaga ggcgagtttc ctgctgctga gtttataacc agcatggagg agatgatagt
     2821 atcgcttata gctgaggtta aaaaacagag tggaacggtt gcaatagaac agaaaaagaa
     2881 aaaaacttat gcaaaaaagt ccgttaaaaa aacagccggt gcaagcattg ctttaaaaag
     2941 caaatcgact gtaaaaaaac agtcagttat aattgaagga ctcgcttgtc catgttgcaa
     3001 aataggaaaa attgtgcgtg ggcgtcaagc atatggttgt aaccgatata aagaaggatg
     3061 tgctttcgtg gtaccttttg aaataagagg aataaaaatc acagataaac attttcatga
     3121 tcttttaacc aaagggaaaa cctcaaaaat aaaaggtcta aaaggtaaaa atggaaatgt
     3181 cgatgtaagt ctttttatac gtaataacca tttggaatat catggtagca aagaaaattt
     3241 ctccgatggt atagctcccg acatgccttg tcccctttgc ggaattggca agctagttaa
     3301 aggacgtttc gcttggggct gtagccgtta taatgatggt tgtaggtatc tgattccatt
     3361 ttagatgctt aattgtcgta ttatgtcaca taataagggt taactacact ggattgttag
     3421 ccagaaaacc ataaatatgt caccttgtaa tctttgaaat atgcgttttt tctaattaat
     3481 gctataaagt caatactttc acctgctttt taaattcagg tctttactgt tctcgagatt
     3541 tgtaattaat gacatgtttt gtgatctgtt aaaaaaaatt actttatttt ttcatattgc
     3601 atattataat aagagagtag gttataatat gatgaaaaat gatttattta taaaatatag
     3661 tgacttcgga aagaaagctt caatcaaggc tattgatcga gctttgcgga aggctaattg
     3721 gaaaaatttt aaggcttctc gagatatatc gccccttagg tcagttttgt ctaaaattga
     3781 agacaaaaaa ggggtcactt ctttgcattt tttttgtact tctaaaatcg aaaacatggg
     3841 tgcaaggctt gcatatcgta ctcctgaata ccgatctaaa aatcgttaca cttctgtaag
     3901 atgtgatggg gcttacgctg cttttttctt ctttgttgat aaagaatgtg atatttcaat
     3961 tatagaaatg cttagcggat tagaagttaa tcgattaacc ctgagagctg aatctgataa
     4021 ttttgacgca agatggagcc ccgatggaca tagaatcgct ttttttatcg atgataaaac
     4081 gttttgtctt ttagatccat ttgcatccga ggtgggcatc aagtattgta ctattgagag
     4141 tgcttgttcc aaaagtggat ggtgtttttc tccggattca acattcgctg cattttctgt
     4201 taatgatcgc tataatggtt gtcctcttac ccgagttgaa ctaaaaaata tgaaaattat
     4261 attatataat gatggtttct ccattcccga gtctctatca caaaggataa atagtcttga
     4321 tcttgtttta tctgaaaaac ttcgatggaa caaagaactc gggataattt atggtacctc
     4381 tggtcttctt ggtgttgttg catcctttga tgaaaattct tgtgagcttg tgtcttattc
     4441 aaattaccgt gtgggtattg tctggagtag cagaagtaat tatcttgccc accttggccc
     4501 atgtgttgcc atgaccgatt ctgataagaa tacagaaata tttcaaaatt ctatagttaa
     4561 caatgcacca attgctgttt tcacatcaga taaaagacgt cttgctctgg tgatgcaaaa
     4621 aaataattta tcttctgata ttcttatata tgacggtaac ttcagaacag gagtagttga
     4681 aacatctatt ttaaatagtg atcaaatttc tgcaggtgcg gtattgtgtt ggagtcattg
     4741 ttctggttat atggctgttt tgacaggaaa aaataggctg caaatttgga atgtgtccgc
     4801 taatccttat ctatcatcaa ctacagaaat tgataatggt gtgcgtgcag tgattgcagt
     4861 atgtaaaaat aattttcttg tcgttgggga tagttggcta gctgttgtag acagcaaaga
     4921 tggatctgta cgttgtaaaa cagactttaa actaaagagt aaacatatgc ttcatcttga
     4981 tccaatgaaa aaaatacatc atttcgtcgc taccgaaggt tcagttgtta atgattttgc
     5041 aaatatatat gagaattttt cttcagatgc tttacaggtc tttcaccggt tgagagttgg
     5101 gaacattatc tgttcgcaac agaccagatt tctgcttaag gaagaacttt gttatatttt
     5161 acataaccgc tatacgtggc cgtttcattg gggggccggt gatcgcgttc aggcagatgt
     5221 acgatttgac tcgattcttg ttggtaatga agatctgatg gctcaaaggc atattataga
     5281 aatagaccgt aaaaatgtct cattttgtat gacaaataat cgattgcact aattcacttt
     5341 tagacttgac cccttgttac atatcaaaaa gagtaaaatt taccaaattt tcgtggcaaa
     5401 gtaaacaata cattaagata gcaccaagaa aattatctct tttatatcga ggaaattgtg
     5461 aatttaatag atctagatat tgaccagcgt ttagtcgatg cattaaataa agataacatt
     5521 attgaaccaa cagtgataca aaccatggct ataggagcgg tattttcaaa aaaatctttg
     5581 ataattgagt ccgcgactgg aaccgggaaa acttttgctt atctgcttcc gataatgagc
     5641 cagatagaca cagtaactga taatcttcag gtgttgatac tggctcctac acatgaactt
     5701 gttatgcaaa tttacgagtt aattaaatgt acagccaatg aagcgaatat gccgatacgt
     5761 tcagctggtt ttattggcaa ggtccacata aatagacagg tcgagaaatt aaaagtaaag
     5821 ccccatgtaa ttgttggaac tcctggccgt atcctcgatc tttttaagat aaaaaaactt
     5881 aaactgcata atgtttctac tgttgtaata gacgaggctt catctcttat gctttctgaa
     5941 tcccgacatg ctgttaaaac aatttctgct tcgttaatgc gtgatactca acgtattttt
     6001 gtttctgctc ttttttcaga tcaggcaaaa aaacgggtat tggaaatatg tccaaaagta
     6061 gaacatattc gagcggcaga aaaaacaaat tctgatattc agcactgtta tatcgaaatc
     6121 accgatagtc gcgaaaagtt taaaacatta cgaaagtttc ttgcagcatt tgataagggg
     6181 aaaactctaa tatttgtaaa aaaaaatccg gatgtagcaa tatttgctaa taaacttgaa
     6241 tttcataagg ttgcagtgct tcaattgtct ggagagatgt cgaagataga tcgacaaaag
     6301 aatcttgcac aatttgaaaa taaaaaagat ggtgttttga tagcgacagc agttgcagca
     6361 agagggctgg atatcgattc ggtggataga gtagttcatt atgatatacc aatagagagc
     6421 tcagattatt tgcatcgaag tggacgtact ggccgtgccg gcagaaaagg tatatctgtt
     6481 gctattgctg atagccgtga ggcaagaaga cttttgcgta ttactgaaga ggctgaaatt
     6541 tctacaaaaa ggatgaaaat gtcaaacggt aagttttgtg agtatattat tgaaaataac
     6601 aaactggagc agaaatgaga aaagttatat ttgtcgctat gtttattatt tttatagttg
     6661 gctatacttt ggtctattct gaggatgaac gtggaagttg tgaaatttac gctgatattg
     6721 tgtgtgatat ttgtggtgaa gatcatacaa gctgtattca gatgcgtgat aaagcagttg
     6781 aaataaagga gcactcccta tgcgccaaag ctgttaaaca tctgccatct gcatataaaa
     6841 atgcttctga tttaagtcgt tcattattga aaaaagctgt ttgtgataaa aaaatgcctg
     6901 agacaaccaa ggctccagga cagccttagt gttttattcg ttttctgtta tgcagtttat
     6961 agggtgggaa aaatccggtt ttgttaaaaa taaaaagagc taatacagct aatcccaata
     7021 ttattccaat aaagccgtgc agtttgttgc caagataaag aatacctaaa gttgattcta
     7081 taacaaaaag gataacttta ttaaatagtc gtatattcaa gatattattg gatttcataa
     7141 cgaccccact atgataaatt atttgctttc gagacaaagc tagtgaaata tgtatctcaa
     7201 gtcaattaaa tacttcatgt atatttaata tggataaata ttcaaatcaa taattttagt
     7261 tatactattt tcctcgatgc tttaaatctt gcaatttaga ttgtttgaat ataatttgcc
     7321 aaatattata cttaattagg agttatatat gagttatgtt tttaacattt taaagggagg
     7381 agtcgttggt cttgcaaatt tgatcccagg ggtttccggg ggaacaatgg ccttgattct
     7441 tggtatttat gaaaggatga ttgcgggatt gaatagtata tctttggatt tgttcctatc
     7501 cttctttaag ctcttgactt tcaagaaaaa gaattgggag ctttttttaa tagagcttga
     7561 aaaaatagat tttaagtttc ttgtctctgt ttttggcgga gctttattgg ctattttggc
     7621 ttttgcttct attatggttt actctttaaa taattatcac gatcagactt atggtttttt
     7681 ctgggggctg gttttaccat cagtatgggt tccgtttaaa ttaataaaaa aaataactcc
     7741 ggcggtttcc atcgccttcg tgtgtgcaac tttgttagtt gtttcaaccg attttttgat
     7801 gagcgacaat gaacaaatac aaaaagagca acaaaaatac gagatagcta tagagaagac
     7861 gcaatcaggc tcgattatgg atgagtttga tattaaccca ttgtcgcttt ttttgctttt
     7921 tgttgcagga gccgtttctg tttcggcaat gattttgcca ggaataagcg gttcatttgt
     7981 tttgttgcta atgggacaat acttcattgt tctaaaggct gtttcgagtt ttaatattgt
     8041 ttatctggca gttttttctg taggccttgt ttttggatta ctctcgttta ctaagttact
     8101 tcattatttg ttaaaaaaat tccatgacat cacgatggga gctcttacag gcctagtttt
     8161 tggttcactt tgggttattt ggccttttaa aacatcctat cttgttggag aggtaggtta
     8221 tgccgatggt tatccccagt tggtttatct gtccaatgta atacctgaaa aattaacatt
     8281 ggtagaactt tcttctttac tatcggtatt agctggaata acagttgttc tggtaatgat
     8341 caaaatagag aaggctaata atcaataata aagattattc agtaatatct atacaaggaa
     8401 ttttaccata aaaagatatt gtgtatttgt ttgcggtatt gagacaatat gataaaagaa
     8461 gagagtgtta catacactct cttcttaata gtctgtgttt aaaaaaacag ttttagatgt
     8521 ctatgtctaa atcaaaatca tcagaagtag ttgcatcttt tggttttggt ttgtcctctg
     8581 tatcggataa gaaatcgtct tcactatcca tgtcaaggtc atcagatgtt tctgctttag
     8641 ctgccggttt gataaccgta gctttgatca tttttgaacg acgaggggta gtgaaatata
     8701 cctcccggta gttgatgttg tgaacaacat caagtttgta gtctttcttg gataattcat
     8761 cactagtgta ttcgcgagtg ttttttgcgg gatccatttc gtaagatgaa tagtcaagtt
     8821 caacataaac agtcacatcg ttttcacggc ttgctaaaga tgtaagatct gcatcaatag
     8881 gaatcaagct tgtatcagtg tatttagtgt cgccaaaagg aactttctca cctttacgta
     8941 cacgtttttc tccaacttgt ttgccgcgac ggtcataaac ataagtaaca acgtcttctt
     9001 gacggtaccc ttcgatacct tctttgggaa cttctacttt tgccaaagct ttttctgttt
     9061 tgctgtcggc aaaaacaagc ataaccttaa gaccctgctc catttcactg ataagctgta
     9121 tgtgatcagc atcagatgtt cctgaaagat ctacatagaa tgtctgaaaa ttatcagcgt
     9181 ctagtattac tcgctcgcca tttttaagtt cgacggggcg ggttgagtcg acgtttttat
     9241 atttcaattc gacatttgtt aggatattgc ctttggcctt agtagtaatt actacatttt
     9301 gaccacaact aaccaaaaat aagagtacta taagtgcgac taaaactttt ttcataatct
     9361 aaccacctgt taaaatactt ccataatctt tactatttac taactgtttc ggcaggaagt
     9421 caactttttt ttactatacc gtgctttaaa tagcagtgtt cagttagttt caggtagtta
     9481 tcggaaacaa agtcaagtaa agctttttag agtggttttg cacgataaat cgtataaaaa
     9541 aagaacaaaa attaagctta aaatgaatgg aaatattaat tgacaagaat tatgtctcat
     9601 tttagtatta ataaattctg aaaacttttt ggggctgtat tggtaattct ctcttggtta
     9661 ccaccttaca gtaggttttc attaatcagt tagtatttat agcagattgc gacatataaa
     9721 gtggtgtctg acgagcaggt taatgattat tgcttgtcta ttcaatcaat agggattatc
     9781 gttatcagtc aatttaaagt tatggggtta tcgtgatgat tttacatgca gcaacagtct
     9841 ttatctgttt gtcaatagtg atgcttttta gacgggcgga tcgagccaat acaagaatta
     9901 tcaagttgaa aagatttact gataactcgt ttaaagaatt caaaaaattg acggattcag
     9961 aaaataggcg ttacaacgat tcaacaatcg agatggattt acttttaaag aaggctcatt
    10021 ctatttctgg ttcaattagg acttcgattg atgatcttga aaaaagattg cacgggctta
    10081 actcagaaaa gttgggctta aaaaaagttg aggatgacct tagagtagta tctgctgccg
    10141 cagatcaggt taattatcag attaaatata tagaaagcgc taaagcagat cttggttctg
    10201 tggtaaaaaa gattaatgct ctcaatgaaa accttataac tatagagaaa aataattcaa
    10261 cactttacca gtcatttaac gactctcttc gaactcgttc ccgagagtta tcggaagaat
    10321 tggctcagga agtaaaaaag cttaaagcgt cagtagggga taaagagagt gtaataattg
    10381 ctcaggcaca ggacaaaatt gacattctgg gtaagagttt tgagcaatcc ataaataata
    10441 tggaaaaggg aataactaca gccggtgatg caattatgca aaacatacgg caaaaacttg
    10501 atgtattaac aagaactgta aataatatag aagagaccgt aaatacctct gaaaaacgtg
    10561 tatatagtga tttgaccgaa gctatcgaaa atgctgagca tgaaattaat cagatatttg
    10621 aaaaaataca aaatgtggaa atatccattg accagtcacg cacaaaatta attgcttctt
    10681 ttgaagaaga gacaaacaag atcagaaaca gcatggattc tctatctttg cattcgattg
    10741 ccaaaaaaga tgaaataata atggcggcac gacaagaagc tgatgaaatt aaaaaaattg
    10801 tagataagtt taacgaacag tataaccgtt ttgaagtaaa tcttaatgcg gttgcgcaag
    10861 agcagatgga gacaattgtt cacgagtatg aatcaattga gtctaacttt gacaaattta
    10921 ctcaaaagct tgaaatgctt gatgaaaaga ttgctgaacg acagcgtatg caggcagaaa
    10981 aaaaccgtga ggaattctct tacatggaag agagactcgc ggaaattaaa aatgaaatag
    11041 tgacttacga aaagagtaat aaggtatttg aacgcacaga tgagatgatg tcgcgtgttg
    11101 aaggtgctct agaagagtat aatgctgttt ttgaaaaggt tcagtctgag tcttccgtta
    11161 tcgaaggttt tgtaaaggat cttgaccagt ttaaagagat gaaaagagaa ctggaaaaag
    11221 agttaaaagt ttacaatgct aaaagagaaa aattgcagaa cgtagcaacc gatatccaag
    11281 ctctgtttga cattgcggaa gatgttagct ccaagagtga atttctggaa gataatgcag
    11341 taaatattga taaggtaaat cgtcgtattg atgatctaaa caatacttat caagcattag
    11401 gggtaaaaat aaaagaactc caagatttcg aaaagaatat agaaaagaat gtttctaata
    11461 ttgatagaat agaagctact gttaactcaa tcgataagcg tgttcagctc tttactgtgt
    11521 cagtcgagaa atctgaaaac aagataaata aactgaaaga gtatctgact agtattgaag
    11581 aagctactta tgttttaaaa actaaagaac acgaaattaa ccatcttaaa gaacgttttg
    11641 aagaggtcga tatgcttgct gcctttgttg acaagcgtct tgatcaacta aaaatagcca
    11701 tgaatcgcat tgaaagtatg cgttcggata tagatagcac agacagtcgc ttgcaagaaa
    11761 tgtttaagga aacagataag cgtatgcaac aattttctga gtttcttcaa acagttgaac
    11821 ctgataatct tattactaag caaatgcgtg gggattttgt tccaggatca aagtcgataa
    11881 atgatggtat ggtgcgcaca gtccgagagt tatcgaataa aggttggacc gcgatcgata
    11941 tatcaaaaaa actgctgctg gatgaaaatt cggtaagatt aattatcaat accgctgctc
    12001 aatagataga ttgtactctg tattcgttaa ttttaacgaa tttatatccc atttcccgta
    12061 gttgtggaac tgcgagtttt aaagcttcag cggtgttgcg tcgagggtgg ttcatatgca
    12121 ttataactat ggaaccttcc tttgctttta atatcttttc agaaatttct gtggcagttt
    12181 tggttttatc aaaatcatga ctgttggtgt caaagttcaa aattttatag cccatcgact
    12241 caataattct aattgaagtt tcatcgtacc atgctgttcc tgatctgaaa tatttgggtt
    12301 tttttgctcc gtattctttg aatattcggg cagacatctc tacctcttcg acaagttctc
    12361 ttatattgct tgtaccacga acaccgtaca tagaagcgcc attgattgaa gctggcttat
    12421 gttttaatcc atggttttca atttggaaaa gaggattact cacgagatca tcaaaagcag
    12481 aacggttttt cttaatccac ctgatgttca aaaaaagagt ggcttttatt ttttcatttt
    12541 tcagataatc aataatttct ttatcgtatc cacacgcgcc acgcccccca catgcgtcaa
    12601 atgttagagc tataaccttt ttatttgttt taagtcgtga ttcagggcaa actaaccttc
    12661 ctgggggaag agatttgtat ctgttttctt tagagacaat aatagagttt aaagaagaac
    12721 aagaatattg gagaataatt aaacatataa gaaaaccaat aagacgagcc ataagaacaa
    12781 ttacctgttt aataaaattt taagatcatc aaatgaaaat ggtttttgaa ttaaagcgtt
    12841 aaaaccgaat ttttggtaat cagcgataac tggattttct gtttgatgtc cggaaatcag
    12901 gatatacgtt gcatcgggaa attcaggttt aagtgatttc aaaagcgata ccgcgccttc
    12961 cgatcctggt atggagagat caagaataaa tgtttttatt ttagggcata gatctctatt
    13021 cgcgtttatg taatctaaag cggttttagg atgtgtaaaa agatgtggag tgtagtgcaa
    13081 tgctttaagc attttttcta gcattatcaa tatgcttttt tcatcatcga caatgataat
    13141 atgtgaacct attggaatta tgtttttgtt ttgaagtttt gtttgtattt cgattggcaa
    13201 gaaaatggaa aacgatgttg gtgactgaag tgatttgaga acaattgttc caccatgggc
    13261 tttaactgtg ttgtaagcat ttgatagacc catgcctcct gctttagatt tggttgtgaa
    13321 gaatggatca aatatctggt ctgcgctttc tgttgtaatg cctttaccaa aatcatttat
    13381 atcaatacgg atatagttac cagattgcag gttgtaagcg gaagtcgagc ctataatttc
    13441 cttagtaatt ataacattta ccgtatttgg gccttgagat gcttctttta catttaaaaa
    13501 catattttca aatacttgac tcatttgatc gggatcaaac tttaaatgat cggtattagc
    13561 attagtgctg atattaagtt taagatctga acctgaaagg ataaaggtac ctgtatctgt
    13621 tattatagag tttaaatcag ctgctttctt gatgggagca cttcctttgg caaaaagacg
    13681 aatctgattt gcaagatcac gcgccctatt tgatgatttt tcaatctcat tgattatttc
    13741 ttgaaccggt ggttctgtag tttccataat cattctgagc attgaaacgt ttccgattac
    13801 acctgttaga atattgttaa aatcatgcgc caagcctcga gcgagtttgc tcattgattc
    13861 cattttgcga tttttaagtt tttcgttgtc ggactgtttt tttaaggata tatcctgaaa
    13921 tactaaaaca gcaccgaata gctgatgacg actatttatc aggggagaac agttttctga
    13981 aatgggggtt ttgtgattat tcttcgatat aagttctctt gattcgcgat atattacaga
    14041 ttctccggtt tccataactg tcattggcgt acagtcaagc aatgctccag attgtggatc
    14101 cgttgtcaga tatatatcct ttatgttttt cttgagtgct tctttcttgt ctagaccgat
    14161 tattttaaga gcctggtcat ttatgtatat aatagtgccg ccctgttcaa cggcaattac
    14221 tgcttcaaga atgctattaa gtgtaaggtt aactagattg cggtttttta cgagttgtga
    14281 ttccaaatcg taacggttta gggccatttc gatagtgata tgcataaaac ggtttgatac
    14341 gggtttaagt aaaaatccat acgggtaggt atcttttgtt ctatcaattg tttcatcatc
    14401 actgcttgcg gttaggtaaa taactgggat attgtgctct ttgattatct tttcagcagt
    14461 ttctatgcca tcaatttcgc ctgaaagacc aatatccatt agaataaata gtggagtgtt
    14521 tgattcgcaa tactgaatcg cttcttcacc gctgctgcaa actattgaat cagggtatcc
    14581 ggctttagag atggccgcgg caatatcctt tgcctcaatg ggattatctt caacaatgag
    14641 tactgtagta tttctcattt gcggctgcct ttgtttagga taacaatatt gataattgct
    14701 gtatggttat tcgtcaagta gttactgatt attaatgtga ttatctatta tgataaaaac
    14761 atttcataat tccgtggtaa atttttgtaa gataaagcct caagaatatg attttttttg
    14821 ataccttctt ttttatcaag atcagcaatg gttcttgcaa ctctaagtat tcgaaaatag
    14881 ctacgtgctg aaagtctcat tttttgtgat gaattaatca ggaaattttc atcttcattt
    14941 gataactgac aatatttacg aacatcttct gtgtccattt gaccattaaa cgagtagttt
    15001 ctatttttga atcgctcaag ctgaataagc cggcattcag tgattctctc tcttattgtc
    15061 gctgatgttt ctgtacctga attgttggtc agtttttcgt aagcaattct aggaacatag
    15121 tgctcgatat caatccgatc aattattggt cccgagattt tactgtaata tgatcgaatc
    15181 atagcatttg tacaggtgca ttctatctct ggatcgaaaa tatatccgca tctacaggga
    15241 ttacatgcac cgaccaacat aaacttagca ggatatacgg acgttccggc tgctctggat
    15301 attgttatca caccatcttc aagtggttgt ctcatagttt gcagaagaaa accctgaaat
    15361 tccgtcaact cgtcaaggaa caggattcca ttatgtgata atgatatctc gccgggcgat
    15421 gcgatacggc cgccacctac aatcgagata tgtgatgatg tatggtgtgg tgatcgaaaa
    15481 ggtggagtag tgattagacc gctatttata ggaagtacac cggcatttga gtgaataatt
    15541 gtagtttcaa ttgcctgatt ttttgttagt tttggcagta ttccttgaat agccttcgct
    15601 atcatacttt tacctgatcc tggaggtccg tacattaaaa aattatgata tccggccgtt
    15661 gcaacttcgg cggctctttt gattccctcc tgtccagcaa tagtcgaaaa atcgattgaa
    15721 aaatattttt gagtctcctg acgggtatcc gtgaaaggtt tggtatttgt tgtcaaaatt
    15781 tttataactt cattaaggga ttttgcggga tatattttga tattatctat agcatttgcc
    15841 tcgtaacgat tatcatatgg aactatgaaa tgatcaaaat tttttttgtg taatgttagg
    15901 gtcatagata ttgttgactg cacagctctt aaagtcccat caagagagag ttcgccaacc
    15961 ataggatacg gagtggtgtg aatgtcggtt tgtccagaat gaacaagaat tgctattgct
    16021 ataggtaaat cgaagtttga gccctgcttt ttaaattcag caggagcaag gttaacaata
    16081 tagtttttcg gaggaaacaa aaatccgctg ttttcgattg cggatctaat tcggtctttt
    16141 gcctctttta ccgatgaatc aggtaggcca acaattgtaa agccgggtaa tcctctgact
    16201 gtatcgactt caacttcaac taaatgtgcg tcaatgccgt taataccaag agatatagtt
    16261 tttgataaca tgtggttcac cgtgtactat agaaaatttg gtttaaagtc ccatgtaata
    16321 gtagacggat ttttttaaaa aaagggcgaa ttttttttat aaagttctta tccagttgtc
    16381 aattgttgca tccatagctc gtaaatatgc gttatgccat ttttttgctt ctttttgggc
    16441 tttttctcct cttttttcgg tgtctttatc aaaaactttg cgacggtagt tatcggaatt
    16501 taagaataca aataatagcc ttttgcctga ttttgtataa aaaattccgg cgagggcatt
    16561 cgcatagtgt attgtgcctg tttttgcata aagtgtaaat tcattatctg gattattgaa
    16621 tcttctttta agtgaccagc cccaacctga aggcaacata tagtattcca attcgcgacc
    16681 agaatatgat tttgagtcag catatattaa aaatgcaaga agttgttccg gagaagttct
    16741 gcttgttgta cttaggcccg aaccatttgt tatagtgaat ccttcccaat caactttagt
    16801 aaatttgctt ttaatgtagt ttaatattgt tttactactt tcggagtagt ttagtttatt
    16861 tcccacaatt ttacttgcac tggatagaaa aagcaattcg gcagattgat tgacgctgaa
    16921 ttcgagagtt gttttaatga ttgatgaaac agtttcccct ttatgttgaa caagtaattt
    16981 ggaacttttt ggagtattat tttgttgtgg gtatggtagg tttattccgt gtattgcagc
    17041 gattttttga aatagcagtg acgtgtgtag tgctgtgttt cgtactggaa gtctatgact
    17101 accaaattca ttaatatttt tacaaagatg ccaaattgtc tgatacttat ctgaaataca
    17161 gctgaatttc tcatgttttt gcagttcctt ttctcctctt tctattttta gataagggag
    17221 atctggagtg acattaaaaa ggatattccc gtctttatca ggtctagtcc agtttacaga
    17281 aatatagtta tattcacatg ataaggcgct aataccagaa ttgtacgctt cgtcttcgtc
    17341 aaaaagtgga ttgataatgg gtattgatgt taaagataaa ttatcataat aaaaatttcc
    17401 agttattgtt gttacccctt ttttttttaa ttttgaaatc atattgacca tatcggatgc
    17461 tgtaaaagat ggatcacctt ctccccttag gtaaatatta ccattaataa ctccatcagt
    17521 aatttcacca tcataataaa cagaagtgtt gtagcggtag ttatcgccaa gaaccataaa
    17581 ggctgttgtt gcagtaataa tttttgcagt agaagctggc ataaagagtt tttttcgatt
    17641 atgagaaaat aatacttttt tttgttgagt atcgtatact atgaaacctg tatttgaagg
    17701 agaaaaatga ttaaaatccg gtacgctatt ttttggtaac gtatcattac cgataactac
    17761 ctgagaaggt ttgaagctat tctcaatttc attagcgaaa gtatggtttc tgttttcgaa
    17821 atttatattg caagaaagaa aaaactgtaa aataagaagt aaaagatagt gtttaaggaa
    17881 atttgtcaca atatacctgt ttttattaaa aatatcggtt tttttaaaaa aaattttaaa
    17941 agtttagaga aaattacgta ttaatctttg gaggttccaa tgattgatac gacaatttgt
    18001 ataaaaccag aattgctaag caaactggaa aaatttgctt tagaaaatgg gatgaaaact
    18061 gtagattatg ctttattatt actaaataaa ttggtcaata gtaaaatata tcaggatttg
    18121 tccaaaaaaa aagcagtagc ataccagcct aggtgcacag atggaatatt attgaaaatg
    18181 catatatatc cagatgataa tttttacgag attcttttag attcaagaaa gatttttaaa
    18241 tgttcagtat cttatttgct cgcgtttgcg attgaagaac atgattgttt ggtagattcg
    18301 gaaaaagatt cgaaaaatta tacaatactt aaggttatag tttgcaatat tcctgttttg
    18361 attatttcat ggggtgatgt ttcttgtcat aaggatatag ttaaggctat agagaatttc
    18421 gaagatggtt aagaggggtg ctgtgcgtac aaaaatatta acaacggtaa tgaccataat
    18481 agtaataatt ttttgtgcta ttatctattt aaccgttaat aaaggtgaag atatttcgga
    18541 tacagcggct ataaatttct cttctaaaat gctaaggcag ggtgaagtcg aagttgcgct
    18601 ctcgattctc aacaaaaaaa ttaaggctgg tacagataat cctttaatat tttataatgc
    18661 tggcgttgtt ctactaaata aaagagagta tagtaaagca gttgaattgt tttcggaatc
    18721 aattcggctt gatccaagcg taagtgcttt aaataacagg gctttctgct acaccacttt
    18781 aaatgaaagt aaaaaggcca tagatgatta tacagcaatt ttagaaattg atgaaaatca
    18841 ggcagcttcg tacttacgaa gagggcagtt gtactttaat ataggagatt ttaagtctgc
    18901 taaggttgat ttagaaaaag cctcttcctt aggtttgaaa gaggcgaaaa aattattaaa
    18961 aaaaatggaa taattccgat aactatctgg ctttttatca tttaccacga actacccaga
    19021 tcttcggatt ttattatcat tagatcctca tcaacagaat caaaatagat aataaaaaca
    19081 tcactaccgt ctacagccat agcaggaaag gtgcctcggc cagtattatt gtcgataact
    19141 atctgggcgg catccaaatc tgtcccatta tagtcacctt tggtaaacat cagaacctga
    19201 ctgtcctcat cataaaaagc aaaaagagca gagcctgagc catcaaaacg aagtgcgcca
    19261 aaataatccc ctgaagtaga tcccggggtg aataaatctc cataagatgt ccagttcgct
    19321 ccgttattgc ttgaatatgc ataacggata gttccagtag catcatttgt cacataagta
    19381 acaccaagat tactgccatc agcattaata gaaggtgcaa gaccatagcc agcgctttcg
    19441 atactctgaa tatcccatgt atgcagactt gttgatttag cataacgaag ctcttgtatc
    19501 gaagcatctc cgttggcaac tgtagttggt actgtaaata gtatgccatc aacagaggtc
    19561 gctatgccat tagtgaaccc tattccgttg ccatcactta ttgtctgttc aaatacagca
    19621 acccgtcgtc cgttatagtt ataggctact atgtcaggat tataggcgtt attaatagct
    19681 gttacaatat tatcaatttg agtgttagca ttatcaggta atgtaatgtt atttggaact
    19741 ccatcaatag ttagagtaaa atcatgagta gaaccgccca aattaaaacc ggttattggc
    19801 gctgcagctt cgatatctat cgaaaatagt gaagcataac tattctctgc atagtaggct
    19861 aaatgcacag tagctcctga aacagctact gatgtaccgc cgcaagtggt tttatccaca
    19921 agataaactt cccaggtagt tccactgtca ttcgaatgag ccagtttaac catgtcgtga
    19981 gtactgtcgt agtacgaaat atataggttt gtaccgttaa gagccatcga gatatgctcg
    20041 ccacaatcag aggagactcc gtcgataact atcg
//
LOCUS       DAOTGZ010000020        19846 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_3064
            NODE_3289_length_19846_cov_4.790511, whole genome shotgun sequence.
ACCESSION   DAOTGZ010000020 DAOTGZ010000000
VERSION     DAOTGZ010000020.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868088
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 19846)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 19846)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:22:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,785
            CDSs (total)                      :: 2,751
            Genes (coding)                    :: 2,732
            CDSs (with protein)               :: 2,732
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 12 of 19
            Pseudo Genes (internal stop)      :: 5 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19846
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3289_length_19846_cov_4.790511"
                     /isolate="AMR_MDS_3064"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..343
                     /locus_tag="P5123_02635"
     CDS             <1..343
                     /locus_tag="P5123_02635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019917133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ATP-dependent Clp protease proteolytic subunit"
                     /protein_id="HRX15188.1"
                     /translation="YIKADVATLCVGQAASMASLLLCAGAKGKRSALPNSRIMIHQPL
                     GGFQGQASDVEIHAREMLSVKSRLNGIMANHTGKTIEQVEADSDRDNFMSAADALEYG
                     LIDRILERKAQ"
     gene            473..1729
                     /gene="clpX"
                     /locus_tag="P5123_02640"
     CDS             473..1729
                     /gene="clpX"
                     /locus_tag="P5123_02640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013276522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit
                     ClpX"
                     /protein_id="HRX15189.1"
                     /translation="MAKKTKDENELICSFCGKNQEIVKKLVAGPGVFICDECIELCNE
                     ILEEDLVSDDQIGSIESLPKPAQLKAVLDQYVIGQEPAKKVLSVAVYNHYKRIISNKK
                     LHKDDVEIEKSNILLVGPTGTGKTLLAQTLARTLRVPFAIADATSLTEAGYVGEDVEN
                     ILLRLIRNADGDIEKAESGIIYIDEVDKITRMSENRSITRDVSGEGVQQALLKLIEGT
                     VANVPPQGGRKHPHQEYLTINTKNILFICGGAFIGLEKIIQGRIATKVIGFDAEIKTK
                     KELKLTGLLSHMQVDDLVKFGLIPELIGRLPVFAALDELDADILKRILTEPKNALIRQ
                     YKKMFELEDVELEFTDEAIREIADIAIARETGARGLRSVLEMIMVDLMYDVPSKEDVK
                     KIIINAEVVRGNEKPKLEQNKTKKSA"
     gene            1746..4160
                     /gene="lon"
                     /locus_tag="P5123_02645"
     CDS             1746..4160
                     /gene="lon"
                     /locus_tag="P5123_02645"
                     /EC_number="3.4.21.53"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009708154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004176 - ATP-dependent peptidase activity
                     [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endopeptidase La"
                     /protein_id="HRX15190.1"
                     /translation="MPVKIDEKKRYPLMPLRDVVVFPHMVIPLFVGRDKSVSALDEAM
                     KSDRLVVLAAQIDPKISEPEGKDVYSVGTICEILQMLKLPNGTIKVLVEGITRVYLTE
                     FYSDEKIYRTDIRLFDYTSEYNADLSEKRSLLLTKFEKYISSNRKIPKEAIKTVSSIE
                     DISHLVDAVAAHINMRLELRQNLIELADPEKRIDRMIELIDGAIEVLELEKKIQGQVR
                     KQMEKTQKDYYLNEQMKIIKRELNNGDDELDEIEELKKEISAEHMPEEIQQKGLKEIR
                     RLEKMPPLSAESAVVRNYIEWLKDLPWTKSTEDNSDISKAKEILDGDHYGLEEVKERI
                     LEFIAVRHLSKSIRGPIICFVGPPGVGKTSLGRSVANALNRKFIRISLGGVRDEAEIR
                     GHRRTYVGSMPGKIIQAIKKAGTRNPVILLDEVDKMSTDFRGDPSSALLEVLDPEQNS
                     TFVDHYIEVPYDLSEVMFITTSNVQHQIPLPLQDRMEIIEISSYTEDEKLNIARKYLL
                     PKQLQSNGITEKHLSLSDEQILYIIRHYTRESGVRNLERTIGKICRKTAREVVEKGDG
                     FEQIIDQEAVRRLLGPIVFKYGEKEKFNDIGLANGLAWTAVGGDVMQIEASLIPGKGQ
                     LMLTGKLGEVMQESAQAAYTYVKANSTFYSIEPKLISENDLHLHVPEGAIPKDGPSAG
                     ITMAVVIASLYSRIPVKCDVAMTGEITLRGKVLPIGGLKEKVLAAHRAGIKCIIIPED
                     NRKDLEKIPANILSELSIHPVSKMEEVLFLALETPQDFFIKEDFRKELFARMESCDSG
                     RVEIQN"
     gene            complement(4345..5100)
                     /locus_tag="P5123_02650"
     CDS             complement(4345..5100)
                     /locus_tag="P5123_02650"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024880.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HRX15191.1"
                     /translation="MKLYSDLAEHYFYIENKHRNISNDVGLIKQLLPRNSRSRILDLG
                     CGTGEHLALLSKEGFLCHGIDNSSEMIENAKKRNGVNISYEIGSMNNFDYFNRFDMVI
                     CLFGSFNYMLTNKDIDAVMCNTYRSLKPGGLAMFEIWNAYPLHKIEHKPLSHVSTTYA
                     HDNKIVRERGFTLTEKQPRTIVDVFYNYHVFSMGKVKRLEDRHTMRAYYQKEIEKFVK
                     DAGFKIIDRFANSMKERFHQFSNKMILVMEKPF"
     gene            complement(5100..6845)
                     /gene="ptsP"
                     /locus_tag="P5123_02655"
     CDS             complement(5100..6845)
                     /gene="ptsP"
                     /locus_tag="P5123_02655"
                     /EC_number="2.7.3.9"
                     /inference="COORDINATES: protein motif:HMM:TIGR01417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008965 - phosphoenolpyruvate-protein
                     phosphotransferase activity [Evidence IEA];
                     GO_process: GO:0006808 - regulation of nitrogen
                     utilization [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate--protein phosphotransferase"
                     /protein_id="HRX15192.1"
                     /translation="MPDKIIRGITASPGIRIGKAFLFKGTKVVIPKYHITDGEIQGEI
                     ERFEDALAKTKKEIVGIQSQVARSLTKDLADIFTSHLMVLEDPMIEQKAIDQVRDEKK
                     NVEWILNDISLELIKSLDSIEDDYLRERILDISDIHKRLIANLQEVEHTTLAKLDEEV
                     IVFAAELTPSDTALLDRDNVLAFVTNAGGKTSHTAIIARALEIPAIVGTRNGTSNIKN
                     GDRVIVDGVKGIILVNPTKDEIRDYVTQQQELLLLEHELSKLTHLPSVTLDDTEIKIF
                     GNIELPEEMSQIQDHGAEGIGLYRSEFLFMDSFLSDEETQYQQYMKVVNFFYPASVTI
                     RTIDVGGDKVYGFTEQYKERNPFLGCRAIRFSLANEDMFRTQLRAILRASHKKNVKLM
                     FPMISTVEEIIWARKITNEIKDELRKEGKDFDENIQLGIMIEVPSAAINSELLAKYSD
                     FFSVGTNDLVQYTLAVDRIGEQVAYLYNPVDLSVLKMLQLINESSIKKSVPVSVCGEI
                     AGEPLYTMVLLGLGFRTLSMASTYMYPVKRIIRSVTIKECEELVDTLMNIETTSDADS
                     YLQNYVREKFPNLVK"
     gene            complement(6958..8139)
                     /locus_tag="P5123_02660"
     CDS             complement(6958..8139)
                     /locus_tag="P5123_02660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014270903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation:proton antiporter"
                     /protein_id="HRX15193.1"
                     /translation="MNLQINTLLAIILGGGWLFSRLFSKIKMPPVLGMVIFGMICSVT
                     IRPYSDPVIYELEPYLKSFALIVILLRAGLGIHREVLKKVGKSALLMAFIPCLVEGTA
                     LIFTIRYFFGFSWEISGLTAFMLSAVSPAVVIPSMLDLKNRGHKEVPTIIMAGASADD
                     VLAITLFSVFLGLSTNSETSAANAVLFVPISLIVGIGSGIITGLFLGAWFKHHHEKIR
                     ATEKTLIILIFATLLVETGNLLHFAALLGIMTCGFILLERHEKIANELANKLSHIWII
                     AEIILFVLIGFSLDISVAYKAGLKGLIVISIGLLFRAAGVILASSFSSLNYKERIFCA
                     IAYMPKATVQAALGGVALANGIPSGSEILAIAVISILFTAPAGLLGIRFFSKRLLKEP
                     Y"
     gene            complement(8246..9292)
                     /gene="argC"
                     /locus_tag="P5123_02665"
     CDS             complement(8246..9292)
                     /gene="argC"
                     /locus_tag="P5123_02665"
                     /EC_number="1.2.1.38"
                     /inference="COORDINATES: protein motif:HMM:TIGR01850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003942 -
                     N-acetyl-gamma-glutamyl-phosphate reductase activity
                     [Evidence IEA];
                     GO_process: GO:0006592 - ornithine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyl-gamma-glutamyl-phosphate reductase"
                     /protein_id="HRX15194.1"
                     /translation="MIKAAIIGATGFGGCGLLEILARHPHIKATQLFAKNETGMKIAD
                     IYPHLEKQYDLEVMPLEKIDYSQIDIAFFSTPDQAGMNLIGEFISRDIKVIDYSGDFR
                     FDTFEEYSVYAINKSMNPKHNSIECLEKSVYGLCEKNREEISKAAIVGNPGCFAISLI
                     LPLLPLCENRLVKTDTIICDSKSGVSGAGKNPGAANFYPQRYENINTYREGSHQHLVE
                     VEMNINKSSKTNYKLFFVPQVIPMTRGILSNIYADIDTSIDQESIIKLYRDYYADAPF
                     VTITDKSPGTAHVRGSNECIIRPLLDKRTGKLLVTSAIDNLMKGQSGNAVQCANIMLG
                     LNETDGLDFPSNYP"
     gene            9590..10597
                     /locus_tag="P5123_02670"
     CDS             9590..10597
                     /locus_tag="P5123_02670"
                     /EC_number="1.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009432370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004073 - aspartate-semialdehyde
                     dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0016620 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, NAD or NADP as
                     acceptor [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0008652 - amino acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate-semialdehyde dehydrogenase"
                     /protein_id="HRX15195.1"
                     /translation="MKKYNVVILGATGAVGLEFRKLLVERKFPIDKIKFLGKSTVGQQ
                     VDFGDTKITVEAVEDGCFDGYDIALFSGGGAISLEVAKKAVAAGCVVIDNSSAWRMDP
                     DVPLVVPEVNAHAVESHKGIIANPNCTTIQMVVCLQAIEKLSHIKKVHVSTYQAASGA
                     GASGMAELEKQFKQIVNGEEPTVSKFAYQLAMNIIPQVDVFTDNGYTKEEMKMFHETK
                     KIMESDIDVSATCVRIPVMRNHSEAIWLETEKKLDPDEVRKAFESAPGVKVIDDPASQ
                     KYPMPLWGEGEDDVFVGRIRKDLTNPKGLTFWCVGDQIKKGAALNAVQIAELLIDKGL
                     V"
     gene            complement(10674..11342)
                     /locus_tag="P5123_02675"
     CDS             complement(10674..11342)
                     /locus_tag="P5123_02675"
                     /inference="COORDINATES: protein motif:HMM:NF016501.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar filament outer layer protein FlaA"
                     /protein_id="HRX15196.1"
                     /translation="MKKIAIVLLILIFSVSLYSQDADTPKETPSDDPTSSALGDQIYQ
                     EVVLEDFEKTEYSDKDVSIRVTRDQKVSIALRDVFPAPVKDSKKYLGVKVYGRQGDYI
                     TVTFPKKLIIEKHCQSISMWVYGKNFSGELSLILQDADKKIHRLSFGKLNFLGWRKLT
                     VRLTKDIRQEDKYLSQHREMAILKLIYNPSSLKRLPSWNFFYVDDISAKVRDKYKDEQ
                     DDDW"
     gene            11604..14921
                     /gene="carB"
                     /locus_tag="P5123_02680"
     CDS             11604..14921
                     /gene="carB"
                     /locus_tag="P5123_02680"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020777576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006807 - nitrogen compound metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbamoyl-phosphate synthase large subunit"
                     /protein_id="HRX15197.1"
                     /translation="MPKRDDIHKILIIGSGPIVIGQACEFDYSGSQACKALKEEGYQV
                     VLVNSNPATIMTDPEMADSTYIEPITPEFVKRIIEIEKPDALLPTVGGQTALNTAMSL
                     YKMGVLDENKVQLIGANVEAIEKAEDRDLFKKAMIKIGADVPKSMIASNLEEAMAVLE
                     EIKLPLIIRSAFTLGGSGGNIVYNKEDYVDLVMNGIDESPINQVIVEESVLGWKEFEL
                     EVMRDTADNVVIVCSIENIDPMGVHTGDSITVAPQQTLTDREYQNLRDLSLKIIREIG
                     VDTGGSNIQFAVNPENGRVIVIEMNPRVSRSSALASKATGFPIAKIAAKLAVGYTLDE
                     IRNDITRETPASFEPSIDYVVLKVPRFNFEKFPGADNRLGPQMKAVGEAMAIGRTFKE
                     SLQKAMRSLEDDRYGLGSDGNIKIDSYLRSLPADKLEDVLEYNLMNPFTGRIFYVKLA
                     LEFGWSVEKVYNLSGIDPWFLYQIKELVDKENFFKEQTKVNGLSVEILKGMKESGYSD
                     RQIAYLLNADSLNTLYSGGAEKQKEYAESMNRFEDDVRSFRMENSIIPGYRLVDTCAG
                     EFEAYTPYYYSSYDSDDESKKSGKKSVMILGGGPNRIGQGIEFDYCCCHASYALEEEG
                     YESIMVNSNPETVSTDYDTSDKLYFEPLTLEDILHIYNKEKPEGVIVQFGGQTPLKLA
                     RRLLQNGVNVLGTSPDSIDRAEDRERFSEVVNKLNLKQPENGMAFTREEAFAIADRIG
                     YPVLVRPSYVLGGRAMAIIYNRDSLADYIENAVDLSPEHPVLVDDFLEEAVEVDVDAI
                     CDGDEVYVAAIMRHVELAGVHSGDSACVIPPVGIAPDMVKLIEKQTEALAFELGVMGL
                     LNIQFAVKGDDLYILEVNPRASRTVPFVSKAKGVPVAKIALKVMTGRKLKDFNLHERK
                     EIDYVCVKEAVLPFKKFPGVDTVLTPEMKSTGEVMGVADNFNEAYYKASLAAGDHFPL
                     NGTVFVSLNKESRDDLVDGIRLLAKAGYKVAATSGTADYLKTAGIGCDKVFKVKEGRP
                     DILDLVKNGDIHVIFNTPAGYRSKHDSNSIRQVAVRYKIPIVTTVPGIKAAVDGMLHM
                     KNNDVVNVRPIQQYHAE"
     gene            15042..15212
                     /locus_tag="P5123_02685"
     CDS             15042..15212
                     /locus_tag="P5123_02685"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRX15198.1"
                     /translation="MDVGSINRSMQSVVQLGDMLKDISARQTGLTEKMIGVAVEQRVS
                     SDTLGTLADYRA"
     gene            complement(15274..15633)
                     /locus_tag="P5123_02690"
     CDS             complement(15274..15633)
                     /locus_tag="P5123_02690"
                     /inference="COORDINATES: protein motif:HMM:NF012241.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp20/alpha crystallin family protein"
                     /protein_id="HRX15199.1"
                     /translation="MTNATESVKYNYITPAADIYEEETKFVLKADLPGVSKEGLDVTV
                     ENNLLEITGKTDPELFIHTDESREEGIYAYKRSFKIDRNIDTGRISASLQDGVLTLEL
                     AKSEEVKPKKIEISYKH"
     gene            complement(15647..16039)
                     /locus_tag="P5123_02695"
     CDS             complement(15647..16039)
                     /locus_tag="P5123_02695"
                     /inference="COORDINATES: protein motif:HMM:NF012241.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp20/alpha crystallin family protein"
                     /protein_id="HRX15200.1"
                     /translation="MLTTYNVLHDINSLLDSFIEPRHAVHELPNVDFEEKEGEINVKY
                     LVPEAKIEDIDIQLENNILKVSYERKQDESERRYIRKERTSNRFSRAIKLPYKVDGDN
                     AQASLKDGVLKVTLKKSPDALPKKIEIK"
     gene            16346..16657
                     /locus_tag="P5123_02700"
     CDS             16346..16657
                     /locus_tag="P5123_02700"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRX15201.1"
                     /translation="MTEALQFNEVFNLVSDFFCVPILILIFSLRELPRYWLFLTALGC
                     VILSHLFTVIESFWYPEYFDIAEHFILLIAGIFFFTGIFKYFFFSSDNDNDNDNDSIN
                     N"
     gene            16626..17549
                     /locus_tag="P5123_02705"
     CDS             16626..17549
                     /locus_tag="P5123_02705"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HRX15202.1"
                     /translation="MIMITIPLIINLASVLIYSSAMILLAIAVFLHKIYWKIAFFLMF
                     CLFSLFLVTLFNSLSLTGVVDNFDLFEEYFEILIIPYFLLYIFMFSSYKEISKRKNRE
                     IKLKKLVSERTVLIKEIHHRVKNNLQTIAALIEMQLDKVENSCAVKELTTINNRIRSM
                     GLLYTMLCRSDNLANVDVGMYIRSVTGYIVESFHLENRIKIEVKTINYIFPIDYTIIF
                     GLITNELVTNSIKYAYKDEQSGEIYISLEKGRDNSFCLVVRDNGAGFNEKPQEHKGLG
                     LVVVEALVKQLDGELSLNHDKGTTWEIKFSL"
     gene            complement(17558..18328)
                     /locus_tag="P5123_02710"
     CDS             complement(17558..18328)
                     /locus_tag="P5123_02710"
                     /EC_number="2.4.99.-"
                     /inference="COORDINATES: protein motif:HMM:NF013915.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0008961 -
                     phosphatidylglycerol-prolipoprotein diacylglyceryl
                     transferase activity [Evidence IEA];
                     GO_process: GO:0042158 - lipoprotein biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prolipoprotein diacylglyceryl transferase"
                     /protein_id="HRX15203.1"
                     /translation="MFPVVFQINLGGTPLVIGSYGIMLGIAFYCAYCLLEREIILNKK
                     NPDLAFYILLAAVLLGLIGAKIFYIFENFQEFRSAPLQTTFSRTGLVVYGGLLLALSG
                     CAIIIRIYKYSVIEVFDLSSPAIAVGFAFGRIGCHVSGDGCHGIEAYNFLTLPYPNGI
                     VPAFSPVYPTSLFESFFYFIIASSLIILRKRKRPSGFIFAIFLISMGLIRFFVEFIRV
                     NPILVLGLTQAQIIAFGLITTGVAILIWQRVHLKEAHS"
     gene            18558..19325
                     /locus_tag="P5123_02715"
     CDS             18558..19325
                     /locus_tag="P5123_02715"
                     /inference="COORDINATES: protein motif:HMM:NF014600.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M15 family metallopeptidase"
                     /protein_id="HRX15204.1"
                     /translation="MKKFIVAVTIVLFYILSLPGAPLIALYGDVDEASYLSGKFNPST
                     HSQFVNLDDLNIDTDGRKHYLRRSTAVALKKMVVAFKKDNPNIRLYVTSSTRNFNYQK
                     AIWDAKFNGSRKVDGKALNKTEPNHLKRALTILQYSSMPGTSRHHWGTDLDFNSLVNS
                     YYENGEGLIIYNWLKKNAPDFGFYQPYTAGRKQGYNEEKWHWSYLPESSIYLRRWMKI
                     FGNDTSFFSKEGSFGGSEYADRLAPIYVMGINPECVR"
     gene            19371..19739
                     /locus_tag="P5123_02720"
     CDS             19371..19739
                     /locus_tag="P5123_02720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015676999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRX15205.1"
                     /translation="MDKKERVALIRKGNELYNNGDYTAAEKIFIKTGYKDGIIRIGDY
                     LYYDQKMPLAAYKYYKLAGCNSKISEIYDRMVFAMKCLMEDKSSEDLRPKIHLEPVDV
                     HPMLKIAAEEILRNNGEIQE"
BASE COUNT         5928 a         3534 c         4314 g         6070 t
ORIGIN      
        1 atacatcaag gccgatgtgg cgactctttg tgtcggtcaa gccgcttcta tggcttcatt
       61 gctactttgt gccggagcga aaggtaaacg atcagctctt ccgaattcaa gaatcatgat
      121 acaccaaccg cttggtggat tccaaggaca ggcttccgat gtcgagattc acgcacgcga
      181 aatgctgagt gttaaatcaa gattaaacgg tattatggcc aatcataccg gcaaaaccat
      241 agagcaggta gaggctgatt cggatcgcga taatttcatg agtgccgctg atgctcttga
      301 atatggtcta attgacagaa ttcttgagag aaaggctcaa taatccgatt gtaacaataa
      361 tatgatattt tttccatgta aagggagtgt ctgatttaaa tatgagtagt gtttcagtta
      421 gatcctgttt gtgggttggc tgaccttgat gatacatgga aatggagtaa aaatggcaaa
      481 aaaaacaaaa gatgaaaacg aactgatctg ttcgttttgt ggtaaaaacc aggaaattgt
      541 aaaaaaactg gtggcaggac caggagtttt tatatgtgac gagtgtattg aactttgcaa
      601 cgaaattctt gaagaagatc tagtttcaga cgatcagatt ggttcaatag agtctcttcc
      661 aaaaccggca cagttaaagg cggtacttga tcaatatgta attggccagg aaccagcaaa
      721 aaaggtatta tccgtagctg tttataacca ttataaacga attatttcta acaagaagtt
      781 gcataaagat gatgtcgaga ttgaaaagag taatattctt ctcgttgggc cgacaggtac
      841 aggaaaaaca cttttggcac agactctggc aagaactctc agggttcctt tcgcgatagc
      901 cgacgcgacc tctttaacag aagctggtta tgttggtgaa gatgtcgaaa atattctttt
      961 gcgacttata cgtaatgccg acggtgatat cgaaaaggcg gagtcgggta taatatatat
     1021 agatgaagta gacaagatta caaggatgtc agagaatcgc tccattaccc gggatgtgtc
     1081 gggagagggc gtacagcagg ctcttttgaa attgatagaa ggcactgtag caaatgttcc
     1141 tccacaaggt gggcgtaagc acccgcatca agagtatctg acaataaaca caaaaaatat
     1201 cctttttata tgcggcggtg catttattgg tctcgagaag ataatccagg ggcgtatagc
     1261 tacaaaagta attggttttg atgccgagat aaaaacaaag aaagagctca agcttaccgg
     1321 actactgtct catatgcagg tggacgatct tgtaaagttt ggcttgattc ctgagttgat
     1381 tgggcgcctt cctgtatttg ctgctcttga cgaacttgat gctgatatac ttaaacggat
     1441 actcaccgag cccaagaatg cccttatccg acagtataag aagatgtttg aacttgagga
     1501 cgttgagttg gagtttactg acgaggccat acgagaaatc gccgatatcg ccattgcccg
     1561 cgagactggt gcgcgcggct tgcggtcggt tcttgagatg ataatggttg atcttatgta
     1621 tgatgttcct tcaaaagagg atgtaaaaaa gataatcatt aatgccgagg ttgtacgagg
     1681 taacgaaaaa cctaaactag agcagaacaa gacaaaaaaa tcagcctgaa aaaggagagt
     1741 cttctgtgcc tgttaaaata gacgaaaaaa aaagatatcc gttaatgcca ttgcgtgatg
     1801 ttgttgtttt tccgcatatg gttattcctc tctttgttgg tcgtgacaaa tcggtcagtg
     1861 ctcttgacga ggcaatgaag agtgatcgcc tcgtagttct cgcggcacag attgacccaa
     1921 aaatttccga acctgagggt aaggatgtct actctgttgg aacaatttgt gaaattttgc
     1981 aaatgcttaa acttcctaac ggcacgataa aggttctggt cgagggtatt actcgtgttt
     2041 atcttaccga gttttatagc gacgagaaaa tttaccgtac agacatacgt ctttttgact
     2101 acacatcgga atataatgcg gatttatctg aaaaaagatc cctgcttttg accaaattcg
     2161 aaaaatatat ttcgtctaat cgtaaaatac ctaaggaagc tatcaaaact gtatcatcca
     2221 ttgaggatat ttcgcatctt gttgatgctg tggcggcgca tataaatatg cgacttgaac
     2281 ttcgacaaaa tctaatagag cttgctgacc cagagaagcg aattgatcgt atgatcgagc
     2341 ttatcgacgg tgcaattgaa gtacttgaac tcgaaaaaaa gattcaaggt caggttcgca
     2401 aacagatgga aaagactcaa aaagattact atcttaacga acaaatgaag ataatcaagc
     2461 gtgaactcaa taatggagac gatgagcttg acgagataga agagttaaag aaagaaattt
     2521 ccgcagaaca catgcctgaa gaaattcagc agaaaggttt gaaagagata cgtcgtctag
     2581 agaaaatgcc tcctctttcg gcagaatctg ccgttgtgcg caactatata gaatggctta
     2641 aagatttgcc atggactaag agtacagaag ataatagtga tatttcgaag gcaaaagaga
     2701 ttctcgatgg cgaccactac ggtcttgaag aggtcaagga aagaatacta gagttcatag
     2761 ctgtgcgtca tctttcgaaa tcaattcgtg gtccaataat ctgttttgtg gggcctccgg
     2821 gagttgggaa aacttcactg gggcgttcgg tagctaacgc attaaaccgc aagtttattc
     2881 gtatctctct tggaggggtc agggatgaag ccgaaataag aggacataga agaacgtatg
     2941 ttgggtcaat gcccggaaag attattcagg ctataaaaaa ggctggaacc cgaaatcccg
     3001 taattttact tgatgaagtc gacaagatgt ctacggactt tagaggcgat ccgtcttcag
     3061 cactgttaga ggttcttgac cctgaacaga atagcacttt tgttgatcac tatatcgagg
     3121 ttccgtatga tctttcagag gtgatgttta taacaacatc gaatgttcag catcagattc
     3181 ctcttccgtt acaggatcgc atggagataa tcgagatttc aagttatacc gaggatgaaa
     3241 aactcaatat agctcgtaaa tatttgctgc ctaaacagct gcaatcaaac ggaattaccg
     3301 aaaaacactt aagcttgtct gacgagcaga ttttgtatat tataaggcac tatacaagag
     3361 agtctggtgt acgtaatctt gaacgtacta ttggcaaaat ttgtcgcaag accgctcgcg
     3421 aagtagtcga aaaaggtgac ggctttgaac agataattga tcaagaagcc gtgcgacgtt
     3481 tgttggggcc gatagttttc aaatatggag aaaaagagaa gtttaacgat ataggtcttg
     3541 ctaacggcct tgcttggact gctgttgggg gagacgtgat gcagattgag gcatccctta
     3601 tccctggaaa aggacagctt atgcttacag gaaagctggg cgaggttatg caagagtcag
     3661 ctcaggctgc gtatacatat gtcaaagcta acagcacctt ttacagtatc gagcccaagc
     3721 ttatttccga gaatgatctg catttacatg tacccgaggg agctattcca aaagatggac
     3781 cttctgccgg tattacgatg gctgttgtta tagcatcttt gtacagcaga ataccagtta
     3841 agtgtgatgt tgcgatgact ggagagatca cactcagagg aaaggttttg ccgatagggg
     3901 gattaaaaga aaaggttctt gctgcgcata gagccggtat aaaatgtatt ataattcccg
     3961 aagataaccg aaaggatctt gaaaagattc cggcaaatat tttatccgaa ctgagtattc
     4021 atcctgtatc taaaatggag gaggttctgt ttttggctct tgaaaccccg caagacttct
     4081 ttataaaaga ggattttcga aaggagcttt ttgccagaat ggaaagctgt gattcaggaa
     4141 gagttgagat tcaaaattaa agatgggctg taaattaatc tattaataaa aacttacaaa
     4201 aaaatgttag aaaaagttgt aaatatcgtt taccaaattg aaatttttga tcttgtgaaa
     4261 aaataatcaa taatttgcaa tattttatgg ttgttttgag gttaagtcat tgcagtttta
     4321 gaagtcggta gagaaaaacc ggttttagaa cggtttttcc ataaccagta tcattttgtt
     4381 tgaaaactgg tgaaatcgtt ctttcattga atttgcaaaa cggtctatta ttttaaatcc
     4441 ggcatcttta acaaattttt ctatctcttt ctgataatag gctctcatcg tgtgtcggtc
     4501 ttccagtctt tttacctttc ccatgctaaa tacatgataa ttgtaaaaaa catcaacgat
     4561 tgttcggggt tgtttttcag ttaaggtaaa acctctttcg cggacaatct tgttatcgtg
     4621 tgcgtaagtt gtggaaacat gactaagagg tttatgttca attttatgca gagggtaagc
     4681 gttccatatt tcgaacatgg caagtccacc tggctttagt gaacggtatg tattacacat
     4741 aacagcatct atatccttgt tggtaagcat ataattgaaa gatccgaaaa gacaaattac
     4801 catatcaaat cgattaaaat aatcaaagtt attcatggag cctatttcgt aactgatgtt
     4861 taccccattg cgttttttag cgttttcgat catttcggaa gagttgtcaa tgccgtggca
     4921 taagaacccc tcttttgaaa gcagagcaag atgctcacca gtgccgcagc cgagatcaag
     4981 tatgcgcgat cttgagtttc ttggaagtag ctgtttgatt agtccaacat catttgatat
     5041 attacgatgc ttgttttcga tataaaagta gtgttctgct aaatcggaat aaagtttcat
     5101 tattttacca gatttggaaa cttctccctt acatagtttt gtaaatagct gtcggcgtca
     5161 gaagtcgttt cgatattcat tagagtatcg acaagttctt cacactcctt tatagttaca
     5221 gatcttatta ttctcttaac agggtacatg tatgtcgaag ccattgataa tgttctaaat
     5281 cccagaccta aaagtaccat cgtatagaga ggctcaccgg ctatttctcc gcaaacagag
     5341 acggggacac tttttttaat gctagattca ttgatcagtt gtagcatttt cagtacggaa
     5401 aggtctaccg gattataaag ataggcaact tgttctccga tacgatcaac agccagtgtg
     5461 tactgaacca aatcatttgt tccaacagaa aagaaatcag aatattttgc tagtaattcc
     5521 gaattgatag ctgctgacgg aacctcgatc atgataccca gttgaatgtt ttcatcaaag
     5581 tcctttccct cttttctcag ttcgtctttt atttcattgg ttattttacg agcccatatt
     5641 atctcttcca cagtagaaat cataggaaac attagcttta catttttttt gtgactggca
     5701 cgtaatattg cccgtagctg cgttctaaac atatcctcgt ttgccagaga aaaacgaatg
     5761 gcacgacaac cgagaaaagg gttgcgttct ttatattgtt cggtaaagcc gtaaacctta
     5821 tcaccaccta catctattgt gcgtattgta accgaagcag gatagaagaa gttgactact
     5881 ttcatgtatt gctgatattg agtttcttca tcagagagaa agctgtccat gaaaagaaat
     5941 tcagaacggt aaagaccgat gccttctgct ccgtgatcct gaatttggct catctcttca
     6001 ggaagctcaa tattaccaaa aatttttatt tcggtatcat caagagtaac agaggggaga
     6061 tgagttagtt ttgataattc atgctctaaa aggagtaact cttgttgttg tgttacataa
     6121 tcgcgaattt catctttggt cggattgacc agaataatcc ctttaacacc gtctacaata
     6181 acccgatcac catttttaat gttggatgtg ccgtttcgtg tgcctacaat agctgggatt
     6241 tcgagcgcgc gagcaattat tgcggtatga gaggttttac ccccagcgtt tgtgacaaag
     6301 gccaggacat tgtcgcgatc gagtagcgct gtgtcagaag gagtaagttc tgcagcaaaa
     6361 acgattacct cttcatcaag tttggcaagt gtggtatgct caacctcttg taaattggca
     6421 atcaaacgtt tatgtatatc ggaaatatca agaattcttt ctcgaagata atcatcttca
     6481 atagaatcga ggctttttat gagctctaaa gagatatcat ttaaaatcca ttcaacattt
     6541 tttttctcat cacgaacctg atcgatggct ttctgttcga tcattggatc ttcaagtacc
     6601 ataaggtgac ttgtgaaaat atcggccaga tcctttgtga ggcttctagc tacctgagac
     6661 tgaattccga cgatctcttt tttcgttttt gccagtgcgt cctcgaaccg ctcaatttcg
     6721 ccctgaatct ctccatccgt aatatgatac tttgggataa caacttttgt acccttgaac
     6781 aagaaggctt taccaatgcg tattccagga gatgcggtta ttcctctgat tattttatct
     6841 ggcatgaata ttttccgatt agaatgattt acgcaatggt aatttatttt taaaaaacat
     6901 gtttgtcaag ttaataatat caaagtacta aaagcaattt tagcgttaaa aaggcgccta
     6961 atatggctct ttcagaagtc gtttgctaaa aaaacgtatg cctaataggc cggccggcgc
     7021 tgtgaaaagt atcgatatca ccgcaatagc caaaatctct gagccagacg gaataccgtt
     7081 tgccaaagct actcctccga gtgccgcctg aacagtcgct tttggcatat aggctatagc
     7141 acaaaatata cgttctttat aatttagact gctaaatgat gaagcaagaa taacccctgc
     7201 agctctaaaa agtaacccaa tagatataac gataagccct tttaagccgg ctttgtatgc
     7261 gaccgaaata tccagagaaa atccgattag tacaaataga attatttcgg caatgatcca
     7321 tatgtgtgaa agtttattgg caagctcatt ggcaattttt tcgtgacgtt cgagtagaat
     7381 aaatccgcag gtcattattc ctaaaagcgc cgcgaaatga agtaaattac ctgtctctac
     7441 aagcaatgtt gcgaatatca aaataataag ggtcttttca gtggctctga ttttttcatg
     7501 gtgatgcttg aaccatgccc ccagaaaaag ccctgttatg ataccggaac cgataccgac
     7561 tatcaaagat atcggaacga aaagaactgc atttgccgcg gatgtttcgc tgtttgttga
     7621 aagacctaaa aaaaccgaaa agagagtaat ggccaacacg tcatctgcag aagctccggc
     7681 catgattatt gtgggaactt ctttatgccc tcggtttttt agatccagca tagatggtat
     7741 aacgactgcc ggtgatactg ccgaaagcat aaaggcggtt agaccgctta tctcccaact
     7801 aaaaccaaaa aaatagcgga tagtaaatat aagagcagtt ccttctacaa gacacggtat
     7861 aaaagccata agaagagccg attttccgac ttttttcaat acttcgcgat gtatgccaag
     7921 accggcacgt aagaggataa caatcaaggc aaaagacttt agataaggtt cgagctcata
     7981 gataacagga tcgctgtatg gccgtatggt tactgagcat atcattccga aaataaccat
     8041 gcctaaaaca gggggcattt ttattttgga aaacaaccgg ctaaaaagcc aaccgccacc
     8101 gagtattatt gcaagtaatg tgttaatctg tagattcata ataccatcca tgaaacagga
     8161 tagggtgctg atagattaat atcaaaacgg tgaattatat tgcagggggt caccgtttta
     8221 aataatatct gattaaaatg ctttatcatg gatagttaga tggaaaatcg agaccatctg
     8281 tttcgtttaa tccaagcatt atatttgcac actgaaccgc attaccggat tgacctttca
     8341 tcagattatc aattgccgag gttacaagaa gtttgccggt tcttttatcc agcaatgggc
     8401 ggataataca ttcgtttgag cctcgtacat gggctgtgcc tggtgatttg tcagttattg
     8461 taacaaatgg agcatcggcg taatagtctc tgtacagttt aatgatggac tcttgatcaa
     8521 tagaagtatc aatatcggcg tagatattgg ataagattcc gcgagtcatt gggataactt
     8581 gcggtacgaa aaacagttta taattggttt tactactttt attaatattc atctctactt
     8641 ctacaagatg ctgatgagaa ccttcgcgat atgtattaat attttcgtat ctttggggat
     8701 aaaaatttgc tgctcctggg tttttaccag caccggacac gccagatttg gaatcgcata
     8761 ttattgtatc tgttttcaca agtctgtttt cacaaagagg taaaagtgga agtataagtg
     8821 aaattgcaaa acaacctgga ttaccgacta ttgccgcttt tgaaatttct tctcggtttt
     8881 tttcacataa tccataaacc gatttttcga ggcattctat tgagttgtgt ttcggattca
     8941 tggatttatt aatcgcgtaa acagagtatt cttcaaaggt gtcaaagcgg aaatcaccac
     9001 tgtaatcaat aacttttata tcacgtgaaa tgaattcgcc aataaggttc atgcctgctt
     9061 ggtcaggagt cgaaaaaaaa gcgatatcta tctgtgaata atcaatcttc tcaaggggca
     9121 taacttcaag atcgtattgt ttctcaagat ggggatatat atccgctatt ttcatgccag
     9181 tttcgttttt ggcgaaaagc tgtgtagctt taatatgggg atgccgtgcc aaaatttcca
     9241 aaagaccgca gccgccaaat ccggtagctc caattattgc cgcttttatc attatatttc
     9301 ctccccatat ttaagtgatt attgccagct cgttaagtaa cacatttata ctatcgatta
     9361 gtttattaac acatccggtg taaagtattt tttgaccaaa tgttgattat ttattgacat
     9421 atgcagactc tgtttcgaat ttagtattgg aaattagtgt ttgtttcgga aaatttctta
     9481 tagtttttta agtaacgata tttagtgata aaattttctg tgttacatag attgaaagtt
     9541 cactgttaat taaaccgctt ttgcggataa ataaaatgtg gagataatga tgaaaaagta
     9601 caatgttgtt attttaggtg caaccggcgc tgtcggtctt gagtttagaa aattacttgt
     9661 tgagcgcaag tttcccattg ataagataaa atttttggga aaatctaccg tgggtcagca
     9721 ggtagatttt ggtgatacca aaattactgt tgaggctgta gaggacggat gttttgacgg
     9781 ttacgatatc gcgctttttt cgggtggggg agcaatttcg ttggaggttg ccaaaaaggc
     9841 tgttgcagca ggatgcgtgg ttatcgataa tagctctgct tggcgtatgg atccagacgt
     9901 tccgttagtg gttcctgaag taaatgcaca tgctgttgaa tcacataaag gtattatcgc
     9961 aaacccaaat tgtaccacta ttcagatggt tgtctgtttg caagctatcg aaaagctgtc
    10021 acatattaaa aaggtgcatg tttcgaccta tcaggcggca agtggagcag gtgcgagcgg
    10081 aatggctgaa ctggaaaaac agtttaagca gatagttaac ggtgaagagc caaccgtaag
    10141 taagtttgct taccagttag caatgaatat cattccgcag gtagatgtct ttactgataa
    10201 cggatatact aaagaagaga tgaaaatgtt tcatgaaaca aaaaaaatta tggaatcgga
    10261 tatcgatgtg agtgcaacat gtgtgcgtat acctgttatg cgtaatcact ccgaggctat
    10321 atggcttgag accgagaaaa aacttgatcc ggatgaagtt cgtaaggcgt ttgaatcggc
    10381 tccaggggta aaggtgatcg atgatcccgc ttcacagaaa tatccaatgc ctctttgggg
    10441 tgaaggcgaa gatgatgttt ttgtcggtcg aatacgcaaa gacctgacta accctaaggg
    10501 cttaactttc tggtgcgttg gtgatcagat caaaaaaggg gctgcgctaa acgcggttca
    10561 gattgccgag cttctaatcg ataaaggact tgtttgatat tagattaacg ttaaaaaata
    10621 aaaaaaaccg gcctatagcc ggttttttat taaatatccg ttgtgctatt atctcaccag
    10681 tcgtcgtctt gctcatcttt gtatttgtcg cgaacttttg cggaaatatc gtctacgtag
    10741 aaaaagttcc agcttggtaa acgcttaagg ctgcttgggt tgtaaatcaa cttaagaata
    10801 gccatttcgc ggtgttgtga aagatatttg tcttcctgtc ttatgtcttt tgtcaatcgt
    10861 acggttagtt ttctccaacc taaaaaattt aattttccga atgaaagacg atggatcttc
    10921 ttgtcggcat cttgaagaat aagtgaaagt tcaccagaga aattcttacc gtaaacccac
    10981 attgatattg attggcaatg tttttcaatg atgagttttt tggggaacgt tacagttata
    11041 tagtcaccct gtcgaccgta aaccttgaca cccagatact ttttggagtc tttaacgggc
    11101 gctgggaaaa cgtctctaag agctatagaa actttttgat ctcttgtgac gcgaatggag
    11161 acatctttgt ctgagtattc agttttttca aaatcttcca gaactacttc ctggtatatc
    11221 tggtcaccca aggccgatga agttggatca tctgaaggtg tctcttttgg tgtgtcagca
    11281 tcctgtgagt ataatgaaac cgaaaagatc agtatgagca acacaattgc tattttcttc
    11341 ataaggtagt ctcctctaaa attttacggt tttaatgccc aaaacgtaaa caaaaagcgt
    11401 aacaggcacc gcatatgcca aaagataaca ttattacagc aaatgtcaac aataatcact
    11461 tggctgttat atacttgcaa aatgataatt aatatgataa tatagtgata aatctttatt
    11521 ttttagactt gaaaaaatgg tgagggctaa acaattggct gtggacactt tctgcaaaaa
    11581 tatctttatc cccggagtat tctatgccaa aaagagacga tatacataaa attttgatta
    11641 ttggttccgg tccaatagtg attggacaag cttgcgagtt tgattactca gggtcacagg
    11701 cctgtaaagc tctcaaagaa gagggttatc aagtagttct cgttaactct aaccccgcca
    11761 ccattatgac cgatccagaa atggccgaca gtacctacat cgagcccata acccccgaat
    11821 ttgtaaaaag aataattgaa attgaaaaac ccgatgcact gcttcctact gttggtggtc
    11881 agacagcatt aaatacagcc atgtctcttt acaaaatggg agtccttgat gagaataagg
    11941 ttcagctgat aggtgcaaat gtcgaggcga tcgaaaaggc tgaagaccgt gatcttttta
    12001 agaaagcgat gattaaaatt ggtgcggatg ttccaaaatc tatgatcgct tctaatcttg
    12061 aagaggctat ggcagttttg gaagagatca aactgccgct tattatacgt tcagcattta
    12121 ctttaggggg cagtggcgga aatatcgtct ataataaaga agattatgta gaccttgtta
    12181 tgaacggaat agatgagtct ccaataaatc aggtaatcgt tgaggagtca gttcttggct
    12241 ggaaagaatt cgagcttgag gttatgcgtg atactgcgga taatgttgtc attgtatgtt
    12301 ccattgagaa tatcgatccg atgggggttc acactggtga ttccattact gtagcccctc
    12361 agcagacatt gacagaccgc gaatatcaga atcttaggga tctttctcta aaaattatcc
    12421 gagaaatcgg tgtcgataca ggtggatcca atatacagtt tgcagtcaat cctgaaaacg
    12481 gaagagtaat cgtaatcgag atgaatcctc gagtaagccg atcatcggct cttgcgtcaa
    12541 aggctactgg gtttccgata gcaaagatag ccgctaaact tgctgttggt tatacccttg
    12601 atgaaatcag aaacgatatt actcgtgaga ccccagcttc gtttgagccg agtattgact
    12661 acgttgtgtt aaaggttccg agattcaatt ttgaaaagtt tcctggcgca gataaccgcc
    12721 ttggccctca gatgaaagcg gtcggagagg ctatggcaat tggtcgtact ttcaaagagt
    12781 ctctccagaa ggcaatgcgt tctcttgagg atgacagata tggacttgga agtgatggta
    12841 atatcaaaat agattcgtat ttacgaagct tgccagcaga taagttagag gatgttcttg
    12901 agtataatct gatgaatccc tttactgggc gtatctttta tgtgaagtta gcgcttgaat
    12961 ttggatggag tgtcgaaaaa gtatacaatc ttagcggtat agatccgtgg tttctttatc
    13021 agataaaaga gcttgttgat aaggagaact tttttaaaga gcagactaaa gttaatgggt
    13081 tgtctgttga aattctcaag ggtatgaaag agtccggtta ttcggatcgt caaatagctt
    13141 atttattaaa cgcggactcg ttgaatactt tatattctgg cggagctgaa aagcagaaag
    13201 agtacgccga aagcatgaac aggtttgagg atgatgttcg atcttttaga atggaaaaca
    13261 gtattatacc tggttataga cttgtcgata cctgcgctgg tgaatttgaa gcctatacgc
    13321 cctattacta ctcctcttat gattcggatg atgaaagtaa gaagagcgga aagaaaagtg
    13381 ttatgatctt gggaggcggg ccaaaccgta ttggccaggg tatcgagttc gattattgct
    13441 gctgtcatgc ctcatacgca ctcgaagagg aggggtatga gtctattatg gttaactcca
    13501 accctgagac cgtttcaacc gattatgata catccgataa actctacttt gagccgctaa
    13561 cacttgaaga tatactacat atatacaata aagaaaaacc ggaaggtgtc atcgtgcagt
    13621 tcggtggaca gacgcccctt aagctggcac ggcgattgtt gcaaaacggt gttaacgtac
    13681 tggggacttc acccgactcg attgaccgcg cggaggatcg tgagcgtttt tctgaagttg
    13741 ttaacaagtt aaatttaaaa cagccagaaa acggaatggc gtttacaagg gaagaggcct
    13801 ttgcaattgc cgatagaatt ggttaccctg ttttggtgcg tccctcctat gtgttaggag
    13861 gccgtgcgat ggctattatt tataatcgtg acagtttggc cgattatatc gaaaatgcgg
    13921 ttgatctttc tccggagcat ccagttcttg ttgatgattt tcttgaagaa gctgtcgagg
    13981 tcgatgttga tgcgatttgt gacggtgatg aagtttatgt tgccgctatt atgcgacatg
    14041 tagagcttgc aggcgttcac tctggtgaca gcgcatgtgt aattcctcct gtgggtatcg
    14101 cgcctgatat ggtaaagttg attgaaaaac agacagaggc tcttgctttt gaacttggag
    14161 taatgggtct tttgaatata cagtttgcgg ttaagggaga tgatttgtat atactcgagg
    14221 tgaatccgcg ggcatcccgt actgtgccgt ttgtttcaaa agcaaaaggc gtacctgtgg
    14281 ctaaaatagc gctgaaggtt atgactggtc gtaaactcaa ggattttaac cttcatgagc
    14341 gcaaagaaat agattatgtt tgtgtcaagg aagctgtgct tccattcaaa aagtttccag
    14401 gtgttgacac ggttcttaca ccagaaatga aatcgactgg tgaggttatg ggtgtcgccg
    14461 ataattttaa cgaagcctat tacaaggctt ccttggctgc tggagatcat ttcccattaa
    14521 acggaacggt ttttgtctcg ttaaacaaag agagtcgtga tgatcttgtg gatggtataa
    14581 ggttattggc aaaggcggga tacaaggtgg ccgcgacctc cggcacggcg gactatttaa
    14641 aaacggccgg tataggttgt gacaaagttt tcaaggtaaa agaggggcga cccgatattc
    14701 tggatcttgt aaaaaatggc gatattcacg ttatttttaa tacacctgcc ggttatcgat
    14761 ctaagcatga cagtaattcg atccgtcaag tcgctgttcg ctacaagatt cctatagtga
    14821 caacagtgcc gggaataaag gcggctgttg acggtatgct gcatatgaaa aacaatgatg
    14881 tggtaaatgt taggcctatt cagcaatatc atgctgaata gtgaaatttt ttaaaaaaat
    14941 actcattaga cttgattgtg gtgtgaagat tgccgaaaat tttatattga cgagttattg
    15001 ggactgggtt tttggcttgg atcctgttcc gggaggtcat tatggatgta ggttcgatta
    15061 accgctccat gcagtcagta gttcagcttg gggatatgct caaggatatt tcggcccgac
    15121 agactgggtt aaccgagaag atgatcggtg ttgcagtcga acaacgtgtt tcgagtgaca
    15181 ctctgggcac attggcagat taccgtgctt aacgaaaaga agagcggcta atgagccgct
    15241 cttttagtct cgttgtctag ttaataatga aaatcaatgc ttataactta tctcgatttt
    15301 cttgggcttg acctcttcgc tttttgctaa ttcaagagtc agtaccccat cttgcagtga
    15361 ggcgctgatt cgtccagtgt caatattacg atcgattttg aacgagcgct tatatgcgta
    15421 aattccttct tcccggctct cgtctgtgtg gataaaaagt tccggatcag tcttgcctgt
    15481 aatttcaagc aagttgttct caactgttac gtccagcccc tcttttgata ctccaggcaa
    15541 atcggctttt agaacaaact ttgtctcttc ttcataaata tctgcagctg gagttatata
    15601 gttatatttt acagattcag tggcgtttgt catggctata cctcccttat ttgatctcaa
    15661 ttttttttgg aagtgcatcc gggctctttt taagagtgac ttttagaacc ccatctttta
    15721 gagatgcttg agcattgtct ccgtccacct tgtatggaag cttgatggct cttgaaaagc
    15781 ggttggatgt tcgctctttt ctaatgtatc tgcgttcaga ctcgtcctgc ttcctttcat
    15841 agctaacttt tagaatgttg ttttccagct ggatgtcgat gtcttcgatt tttgcttcag
    15901 gaaccagata cttaacgtta atttcgccct ctttctcttc aaaatctacg tttggtaatt
    15961 cgtgaacagc atgacgcggc tcgataaaag agtctaaaag agagttgatg tcatgaagta
    16021 cattataagt ggttaacatg gcccacctcc tataaataag tgctttgtta ttagcactca
    16081 tctttattga gtgctaataa caaattagtg actaatctct aaatcgtcaa taattttttt
    16141 aaattttttt agaatggtga ttttttttta aatattattc ttaggagatt aagcttaatt
    16201 gaatatcttg tcaggtgtat gaatttaagt gaaagaattt atatccgcta atttcgtttt
    16261 cgtcttgatt ttgttttcat acctattata atactattat gaccggcttt agttttgccc
    16321 aataattaaa tatacggtaa aatatatgac agaagcactt cagttcaatg aggtttttaa
    16381 ccttgtttcg gatttctttt gtgtacccat tctaatcctt attttttctt tgcgagaatt
    16441 gccgcggtat tggctatttt taacagcact tggttgtgtt atacttagtc atctttttac
    16501 cgtaattgaa tctttttggt accctgaata ttttgatatt gcagagcatt tcatactgtt
    16561 gatagctgga attttctttt ttacgggtat ttttaaatat ttctttttct ccagtgacaa
    16621 tgacaatgat aatgataacg attccattaa taattaatct tgcttcagtg ctgatatatt
    16681 cttcggcaat gatccttttg gctattgctg tatttttgca taaaatctat tggaaaatcg
    16741 catttttttt aatgttctgc ctgttttctc tctttctcgt tactcttttc aactctttgt
    16801 ctttaaccgg agtagtcgat aattttgatc tatttgaaga gtactttgaa atactcatta
    16861 ttccatattt tttgctctat attttcatgt tttcgtctta taaagagatt tcaaaacgaa
    16921 aaaataggga aataaaactc aaaaaacttg tttccgagag aacagtactc atcaaagaga
    16981 ttcaccatag ggttaaaaac aatctgcaga ctatagcggc attgattgaa atgcagttag
    17041 ataaagtgga aaacagctgc gcagtcaagg agttgaccac cataaataac cggatacgct
    17101 caatggggct tctgtatacc atgctttgcc gttctgataa ccttgcgaat gtcgatgtcg
    17161 gtatgtatat ccgtagtgtt accggctata tcgttgaatc atttcatctg gaaaacagaa
    17221 tcaaaattga agttaaaacg attaattata tctttccaat cgattatact ataatcttcg
    17281 ggttaatcac aaacgagctg gttacaaatt ctattaaata cgcttataaa gatgagcaat
    17341 ccggtgaaat ttatatttct ctggaaaagg ggagagacaa ctcattttgt cttgttgttc
    17401 gggataacgg ggcggggttt aacgaaaaac cccaagaaca taaaggcctt ggactggttg
    17461 tagtcgaggc gcttgtaaag cagcttgatg gagagctctc tttgaatcat gacaaaggta
    17521 caacatggga gataaaattt tctctatgag aaactgttta tgaatgggct tctttaaggt
    17581 gtactctttg ccagattaaa atagctacgc cggttgtaat cagtccaaac gcaattattt
    17641 gagcctgagt tagacccaaa acaagaattg ggtttacacg tataaactcg acaaaaaaac
    17701 gaatcaaccc catggaaatc aaaaaaatag cgaaaataaa accgctcggt cgttttctct
    17761 tgcgtagaat aattagcgaa cttgcgataa taaaataaaa gaaagattca aaaagtgatg
    17821 tggggtaaac cgggctaaaa gcgggaacaa taccgttggg atagggcagt gtcaaaaaat
    17881 tgtatgcttc aataccgtga catccgtcac cagaaacatg acacccaata cgtccgaaag
    17941 caaacccaac agcaatagcc ggtgaactca agtcaaatac ctctataacc gaatacttat
    18001 atatacgtat aataatggca cagcctgaaa gagctaaaag aagtccaccg tatacaacaa
    18061 gccctgttcg cgaaaaagtg gtctgtaagg gggccgacct gaattcctga aaattctcaa
    18121 atatgtaaaa gatttttgca ccgataagtc caagtagaac tgctgcgagt aggatgtaaa
    18181 aagcaagatc aggatttttt ttattaagta taatttcacg ttccagtagg cagtaggcac
    18241 aatagaaagc gattccaagc attatgccgt aagaaccgat aacaagtggt gtgccgccaa
    18301 gatttatctg aaaaactact ggaaacatga ttttctcctg agttggtttg tttttattgt
    18361 gttaactgtt aaagtatatg caaagaattg tcgggagtaa taagtttgta aagcctttta
    18421 gtggtaacaa gaaaaattat atagagtgcg gtctgcttat cgattgactg tttgtaacta
    18481 tagttataag cggtttctat aatagaatat actatttaca gagaaacaga gttctgatgt
    18541 ttatatacgg agtgtttatg aaaaaattca ttgtggctgt tacaattgtt ctgttttata
    18601 tcttgagttt gccaggggct cccttgatag ccctttacgg tgatgtggat gaagcaagtt
    18661 atctgtcagg gaagtttaac ccttctactc attcacagtt tgttaatctt gatgatctga
    18721 atatcgatac cgacgggcgt aaacactatt tgcgtcgttc gactgctgta gccttaaaga
    18781 aaatggttgt agcttttaaa aaagataatc caaatattcg tctatatgtg accagttcta
    18841 caagaaattt taactaccaa aaggcgattt gggatgccaa gtttaacgga tcccgtaagg
    18901 ttgatggtaa ggctttaaat aaaaccgagc ctaaccattt aaaaagagcc cttactattc
    18961 ttcagtattc ttccatgcct ggtacttcca gacatcattg gggtacagat cttgatttta
    19021 attcacttgt taattcttat tacgaaaatg gtgaggggct aataatatac aattggctta
    19081 aaaaaaatgc tccggatttt ggtttttatc agccatatac cgctggtaga aagcagggat
    19141 ataacgaaga gaagtggcac tggagttatt tgccggagtc gtcgatatat cttcgacgtt
    19201 ggatgaaaat atttgggaat gatacttcgt ttttttctaa ggaaggcagt tttggtggtt
    19261 ctgagtatgc ggataggctt gctccgatat atgttatggg tataaacccg gaatgtgtta
    19321 ggtagagcgg tttttttgtt tattgaaaat ccggttacga tgaggtagat atggataaaa
    19381 aagagagggt ggctctgatc cgaaaaggta acgagttata taataatgga gactatactg
    19441 ctgcagaaaa aatctttatt aagactggtt ataaagacgg aattattcgt attggtgatt
    19501 atctctatta tgatcagaaa atgccccttg cagcgtataa gtattataag cttgctggct
    19561 gtaattcaaa gatatctgaa atatatgatc gaatggtctt tgctatgaaa tgccttatgg
    19621 aagataaaag tagtgaagac ctgcgcccaa aaattcatct tgaacctgtt gatgttcacc
    19681 ctatgttaaa aattgccgcc gaagaaattt tgcgaaataa cggagaaata caggagtagt
    19741 tgagtctggt atgtccatga atgtgtccct agtgttgtat gaagtggcgt tgggttttga
    19801 tatattgttc aagtgttgtt agctttgtca aaactgtttt tatctc
//
LOCUS       DAOTGZ010000021        19650 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_3064
            NODE_3333_length_19650_cov_5.065425, whole genome shotgun sequence.
ACCESSION   DAOTGZ010000021 DAOTGZ010000000
VERSION     DAOTGZ010000021.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868088
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 19650)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 19650)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:22:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,785
            CDSs (total)                      :: 2,751
            Genes (coding)                    :: 2,732
            CDSs (with protein)               :: 2,732
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 12 of 19
            Pseudo Genes (internal stop)      :: 5 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19650
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3333_length_19650_cov_5.065425"
                     /isolate="AMR_MDS_3064"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..399
                     /locus_tag="P5123_02725"
     CDS             <1..399
                     /locus_tag="P5123_02725"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRX15206.1"
                     /translation="RTGNIIKCYTTDWNDTENYVAASEITIDLDSDLDLAKFKGAMPY
                     GYVSFSQPNSTYEDINIEGGLDASTMSLINWGEGGTIEVWRYDEMQSQWVLDESSTLQ
                     DVYGYVREVVNPVTGARYLIKQNSVEVIEE"
     gene            574..1767
                     /locus_tag="P5123_02730"
     CDS             574..1767
                     /locus_tag="P5123_02730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013388068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HRX15207.1"
                     /translation="MKKEVKIYFVILALTALGLALSHDLFSNYFKDAYNVTTLQRGSI
                     ELPREFPGVLCFLLVAMLSFISDLKIAFIAQILAFAGVFVLGFVTPSFLWMLFFLFIN
                     SMGMHLFLPLQDSIALSLVDKNRVGKRMGQFKGTMTAFQMIGALIAFVGFKSGVFSFT
                     GKIKHIFIISGFIFLVVAVLFLVLDRMINHKGIHHVKTRFVVRKEYSLYYVLVVMFGV
                     QKQMMMVYAPWVLIELLHKKADTISMLYMAGSFAGIFFIPALGRWVDRFGVRKLLFAD
                     AISFIGVYFAYGALVMGFTGGFFAMAGLPVLAAYLLFIVDRMSTQMAIIRVVYLKQIA
                     LTEKDITPTISLGISVDHVVSISCAVIAGWVWTQFGAHYLFFGVGALSFVNLWVAFRV
                     KESRK"
     gene            1829..3103
                     /locus_tag="P5123_02735"
     CDS             1829..3103
                     /locus_tag="P5123_02735"
                     /inference="COORDINATES: protein motif:HMM:NF040521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C45 family autoproteolytic
                     acyltransferase/hydrolase"
                     /protein_id="HRX15208.1"
                     /translation="MKKILSLLLVFLFVGCNDSGNKDKPSDISTDNISLVDNGSYYQV
                     VLDYRDKTPYEMGQQYGTVISSKVEGYDKLIDPYLIDLANNSGMDFSNSVILSIVSKR
                     INDIKTQIPKEYMDELDGLASVLVNSDSMVIGDGKLSKEEFLLVSLLPDVVRGTACSA
                     LSVFGDRSESGSPVVARVLDWFGGNSDQLVKIQAVVVYKKSTGSVCTVGYLGFMGVIT
                     GFNDKGVFAGILDSPTGATYNSEGKYSYPFDIRYALENNDSLQTTADYLSKHPYTYSH
                     LVFLADKESSKVLENNISDDFRQLREDMSPLNGGIVWGLENTVAAVNSFLLKDNFDNH
                     TVYTGNYNRWEYFYNQISALAGQKLDSEDLKTLITYRSDSGALHQYGSIYVTSTQQMI
                     LFSPESFKLEVFFRPEGGLPETPVFEDIAVTW"
     gene            complement(3113..3727)
                     /locus_tag="P5123_02740"
     CDS             complement(3113..3727)
                     /locus_tag="P5123_02740"
                     /inference="COORDINATES: protein motif:HMM:TIGR01352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0031992 - energy transducer activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="energy transducer TonB"
                     /protein_id="HRX15209.1"
                     /translation="MNRFLVSLYASVIAHLAVFGIAKYYSDTNFFTKPSIKQQNSLQE
                     VTVNIISNSSEFTEQKPTETIDKKNKSNFTVIFSNKEASFFIKNETLEINEDIKTKKP
                     GSKKNNSDLSALTRLADIIAHNNPPNYPYSARRFGHEGEVILEIEITSDGKSGHVSVI
                     KSSGYPSLDRAAIDAAKGWIFFEGESPFNGSSVKITQTIRFKLN"
     gene            complement(3724..4920)
                     /locus_tag="P5123_02745"
     CDS             complement(3724..4920)
                     /locus_tag="P5123_02745"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRX15210.1"
                     /translation="MKIISTYIAALLLIVLLAACDDDKPSKTELPIIDYAFVLTSDYT
                     TGSFSLIDLNSYKTFENPTPGYVHADASVRIFNNIVYIINRAGRDSIQVLDPKKNHEI
                     VTEFSTGTSSNPQDIAIYRDTSGKNKAYVSVYGNSKLLVVNPETGKKSGSIDLSSYSA
                     AGMNHPHMCMMHVHENLLFVALQRLDSSFKVSNYSSVVVIDAEQDIVIKEIRLSWTEN
                     DETVNALNPYSRFKIAPDYINNSGKELIYISCPGEFGYFYKEDGGVVAIDPETLECLP
                     GYLVSESDLNTEITDFQFGPDQKLYILTSSSDFRSNLLLYDLQNESVSKTILSNKGTN
                     GNLCSLSLHTANLLFVSDRTATNPGIHIIDISEDPKHLFDGKPLNTGLPPVMVDFNIS
                     PGRNIR"
     gene            complement(4954..7116)
                     /locus_tag="P5123_02750"
     CDS             complement(4954..7116)
                     /locus_tag="P5123_02750"
                     /inference="COORDINATES: protein motif:HMM:NF019335.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor plug domain-containing
                     protein"
                     /protein_id="HRX15211.1"
                     /translation="MISTPLETRYTTLIQPWDLSVLILTQLAKLIVNSNFWQPFITII
                     IIFEFCCCTILCAQEENNAITVFAPRLTETEKLAQGVNPVTIIVPDSSAVQSSSVSQL
                     LNRVAGIKINRTGGAGSRSMVSIRGSNYNQILICVDGIPVTDPLHGEIDIDSILVASI
                     KRIEIYKGITPLRFGKTGMSGVINIVTDSSEVENLSANIFQNGKSENRIKTAIGSFDT
                     YHLSFKRLSSTKKYSYLLFTAYDSCKNDFYFHNDNGTPVINTEDDKTEKRENSQYFAG
                     KVNLNGKYNFDSFSLSLNNGFMYKKNGVSGINSNTALDSSFTSKKYDSSAAFDFNHFG
                     STSNRLDAKINYSYREQILSDPENEIMMTNKKETGTFHTAGCALYFNSVIDKLNSIFD
                     VTASYSRETFTKKTEDYYDDSTTYPTQKRDNYAGGIGNEFSFFDGRISVLPACRITVA
                     HDNFYTQRTVLDNSPEKDTNNRYLLHYSTSARLNIIDRKRTSLAFFASFSQTGRHPSF
                     MELFGNENGVLGNPDLADENAFNREAGISLSSSENKVLPVIKFSTLFFYNTIDDTILF
                     IQNSQSTMLAQNIGKALIYGTEVSGEVLVKKRLKLSGEYTFQHTEDQSDIPYYKGNQL
                     PGRPTHQFNGLSRLIFQNNELQYSIDVIGSNYLDRANSNFYYIDRRIYHNLLWEIRPL
                     KDLSLIAEINNLLDKKSRDTIGYPLPGRAFYLTAEYLF"
     gene            complement(7183..8073)
                     /locus_tag="P5123_02755"
     CDS             complement(7183..8073)
                     /locus_tag="P5123_02755"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRX15212.1"
                     /translation="MRNFLPIVILTLYVSSSTGCDSGTASDKKESPSETETAPAATLC
                     DGSSADGSLSPEAITMLPLPDYCGTIITTGTKITFKSLSDNIEISVTQDAEATPVWQS
                     TNDFTFNETGTFYLAARPSADCADSDIYRRNYTVLDSFFGAADTPNNNAVHMDSSVLT
                     GWATGYKDYLPSKDVSDEWKDPVLATGKASGTSTDIVSLGNNGSIVLTFNQPITNGTG
                     IDFAVFENSFSNYFLELAWVEVSSNGKDYVRFDSISLTEEPVDGYGTIDPTLIHGLAG
                     KYRQGYGTPLIFPSSRIIEQ"
     gene            8399..9394
                     /locus_tag="P5123_02760"
     CDS             8399..9394
                     /locus_tag="P5123_02760"
                     /inference="COORDINATES: protein motif:HMM:NF013465.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ABC transporter substrate-binding protein"
                     /protein_id="HRX15213.1"
                     /translation="MKYRSVLLLFLLSSGFIMSCSRLPQDNSDANIIYVSILPQKYFA
                     DRICGDKFDVRVMVQPGKSPATYEPLPAQITELSKAKMFFAIGVPFENSFLPKIRSAI
                     PALAIIDTSCSIAKRRLEDHDHHDDHDPHEKNIAGDLKITGDSCNSIKSDIKKHDAKQ
                     DLVKRSNHGKSAEDPHVWMSPVLATVVAQTMLDEIIKIDPGNEDYYKQNFLLLEADLE
                     SLHKELTESLKSLKGKTMLVFHPSFGYFADEYKLKQVAIEAGGKEVEPAGLVKIIEFA
                     KKEKVSMLFVQPEFSQRSARAVADAVGAKVVVASPLDYEYITNMRKIAIRLRELK"
     gene            9391..10137
                     /locus_tag="P5123_02765"
     CDS             9391..10137
                     /locus_tag="P5123_02765"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HRX15214.1"
                     /translation="MTENNIIEIKDLSFAYGNESILSHINCEIKDKELVSIVGPNGGG
                     KTTLVRLMAGLLYPVKGKVYIEGVSTTERHPIVGYVSQNSMIDDKFPVTVQDIVLSGT
                     MKSGFGFYNKNDRKKANSALESVGMSNFVKKRHSDLSGGQRKRVIIARALAGGAKILL
                     LDEPTANLDANSSADLIELIKDLNKTITVVLVTHDYGFVSDITNRVFCINNHLHEHPL
                     KEAEERFAMNLKRVDHTVVLNKCKGCEGKQ"
     gene            10134..11021
                     /locus_tag="P5123_02770"
     CDS             10134..11021
                     /locus_tag="P5123_02770"
                     /inference="COORDINATES: protein motif:HMM:NF013143.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_process: GO:0030001 - metal ion transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter permease"
                     /protein_id="HRX15215.1"
                     /translation="MSSFIAALLNPDMVFIRYALFAGLISAVPFGITGSFIVVKRIGY
                     VAGAISHAVLAGIGFAMYVNAKYDKEVLSPMLGAFIAAVTAGIIISFVSRYAKEREDT
                     IIGTVWAFGMAIGLLFIHSTPTYIDPMSYLFGNIQLLGSLELKLIIILAVIVVFVVVL
                     FYNQLLLVSYDPEFAALRGLNVYFYQVMLIMLISCTVVLMASSVGIVMVIALLTIPSG
                     IASFFTIKLWHMIIVSSIVCAVSVVSGLAFSYMLAMPSGAVIIVVCGIIYFAAMMLNA
                     IFGIIQKNRAVGKRDQIKP"
     gene            complement(11006..11896)
                     /locus_tag="P5123_02775"
     CDS             complement(11006..11896)
                     /locus_tag="P5123_02775"
                     /inference="COORDINATES: protein motif:HMM:NF012472.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HRX15216.1"
                     /translation="MRNLNSHPRFGLGCWPLCGKGWKGATSESAINLIIRAIELGVVF
                     FDTAPVYGFGLSEKLLGEALDRTPSSISNKIVVATKCGLKIDNGRVTHDLSYDFILRD
                     CEESLKRLNRESIELYQLHWPDPQTPLDESFKALTRLLDEGMVKKIGVCNFDIPRLEE
                     TLKLGPVCSSQNRYNFLQQESEELIDFCYDNRIDFIAYSPLAQGLLTPAVNEEYRFAK
                     NDIRRLNPLFDINGNICTKPDDTKGTNFTQCLKKRNSLGDNTVKEALKFVTNNKKISR
                     VLVGTSRTKHLEEILNYGFI"
     gene            complement(11880..12758)
                     /locus_tag="P5123_02780"
     CDS             complement(11880..12758)
                     /locus_tag="P5123_02780"
                     /inference="COORDINATES: protein motif:HMM:TIGR00180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000910 - cytokinesis [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ParB/RepB/Spo0J family partition protein"
                     /protein_id="HRX15217.1"
                     /translation="MAKRLGKGLGAIITTAPPTRAADHVEETIAHVAGESVVEIPLDN
                     ITPNPDQPREHFDEIEIENLAESIRSVGLIQPVLLRKSGDKYIIIAGERRFRAAKVLE
                     MDTIRAIVVKATEEENVTIALIENIQRQDLDPIEEAKAYKVLINRFNLKQNDVAKRVG
                     KERATIANSLRLLSLPDIIINAISAGQISQGHAKVLLGVPGSQKEYYFNQILSQNLSV
                     RALENIIKADQDKLSGESKAKKPHKDPHIRKMEEKLVSTLGTKVEIKHSSTGKGRIEI
                     NYYSLDDFERILEKFE"
     gene            complement(12751..13512)
                     /locus_tag="P5123_02785"
     CDS             complement(12751..13512)
                     /locus_tag="P5123_02785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008497649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HRX15218.1"
                     /translation="MGRVISVSNQKGGVGKTTTTVNLAAFLGAKGKKVLIIDIDPQGN
                     AGFGVSVNAEELQTTIYEVLIGEIGIEEAIFNTEYPNLDIVPANIHLSGAQIDMMDLE
                     GKEFKLKEAVKKIADFYDYVLIDCPPSLGLLTLNALVAADTVLIPLQCEYYALEGLSQ
                     LLRIISMVQENINKQLGIEGVVLTMYDSRTKLSQHVVTDVKEYFKDKVFETIIPRNVK
                     LSEAPSFGQPIGVYDSGCPGSETYEKLAEEVINNG"
     gene            complement(13554..15482)
                     /locus_tag="P5123_02790"
     CDS             complement(13554..15482)
                     /locus_tag="P5123_02790"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRX15219.1"
                     /translation="MHQSNMRLEEKTKRMIARKDIPDSKMEAVNALLKNAGLQPHEKF
                     NAVIQLIQDCPDKPRPVKSVTASKPKTTILPKSASRRTETPVKLSKKTTVIPSPASGS
                     YHVNALLKAYKETGLFKKRYLIESSNFLKFWIKKRIVPSRKLFRVFRTIYSYQEKIAA
                     VLPQVMEDIVNDPAITSPIIFNHLRLLRKWMLNIPFLSAGYNQCLWFDTAAFEAELLP
                     YARMVFSFSMIETELRENIILMFESKLREQGDYKKDIISENDPESSSKQKRNLAKEKA
                     VYDIIVTLRSFMPDANTEGSLDRYLKNATKIANLRDLTVVLVETLIFRRQMSRTELKR
                     YYSIKAPDADPTRWECSRELLKNAGKDSESLRKASLEALKKELEPYDEMVELINLKID
                     GQELTYKAFSDNWHIFSNKRQEFGNYPEENFFFFLDDCTNYFVHVYMPLLNGSPQKFV
                     SSDQTTFEGAIFTPGYFESKISMLLDIQRDLFAYRTKNPNSLISLDEVRKIQQGKLAT
                     MTHIKSFVDHMGAVFYAFAVELHAVYRAHQRFMELHPSTRPESSKEPLRPGVVHDHED
                     DGFLLPFYDMKIAKQDKLPPLARTIVGKYIMSDSVRDGLFKCMLSFCYQMAYECRFLS
                     LEHDLDRRTEIRRSIEAI"
     gene            15743..16687
                     /locus_tag="P5123_02795"
     CDS             15743..16687
                     /locus_tag="P5123_02795"
                     /inference="COORDINATES: protein motif:HMM:NF038353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FxLYD domain-containing protein"
                     /protein_id="HRX15220.1"
                     /translation="MSNLFRCSSCGSPDVRLNKQNQYQCSYCGSLYVHKERASVYAAK
                     EIGNNKSKILLVSAAILFITLLSIAVYYVSVENRAGKNQTVNINSVSVNQDKEDPNKT
                     TVDGSVARQDVLTDNTPGAAKKAEAEVGPLAAVRDSIGGIYFSGFYQNIGEAVITYPK
                     VTVVLFDKYGKELKRAAGYGTRSFLLPGEKTIVRALVSGAPNYDKIEVLCEPQSASKF
                     TRLSRIKLTILESKMNFSGRGRLDITGKVKNIDNKDCRMVKIEALVYKEGKVIGSGLA
                     HITDTIRSSETATFSMYIPSKIDSADSFVLDWQGTPVR"
     gene            16805..18328
                     /locus_tag="P5123_02800"
     CDS             16805..18328
                     /locus_tag="P5123_02800"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRX15221.1"
                     /translation="MNNIKHWLDKTSERGLAYHFFLFLIVAILLKLVYLFASPIWRVD
                     IFFYIQGAKTMLDGGVLFKNFVEIKPPGIFFIYYWLSLVFGYSNILVSIKLLALLSQS
                     LTALFVSTTASRIYTKKTGFAVGMAFLLSSSICYSFWPPNIMLIALTPLLVSIWLCFL
                     YIQKPMIAYLFFTGLCMGISSVISTNLILFTLIVPILCLMSYRSLKKFIAGCLIAFAG
                     FIVVWLLLAVHFYLNNALTDWLWWNFKWASIYGGTRPIYLRIFNFFWGMIRSWQILPL
                     YVLSFYSLYIITKKRLYQNHQDYLVLGILFVISLLSRLAFGKSEPRYYLYMVPFMVLL
                     LPLAIKGFKKILVKKTTFIILAYIIIALSYTSFEGISKPYRRIVTRKVLHEAIKEFSE
                     PTDRIFVWSEGYEIYLMSERSMAGSFFSPAQHLANGYIWMRNGFRNIDVPWQKFMIEF
                     ATDQPELIIDLTGNFKGEGEEYYDKSLFGYMKSFIKEVNEKYSIVSEFGSAVIWKKK"
     gene            complement(18391..>19650)
                     /locus_tag="P5123_02805"
     CDS             complement(18391..>19650)
                     /locus_tag="P5123_02805"
                     /inference="COORDINATES: protein motif:HMM:NF013080.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LTA synthase family protein"
                     /protein_id="HRX15222.1"
                     /translation="TYPFFKHNIKKSNNVDLKRHSTDRPSIIFIMIESFNASVVNSHT
                     ESGKEITPNFNRLVKDGLYIPHFYGNSIQTCKGQYAALFSGIPSYKGKVFVHYKNNDF
                     ISLASVLKNNGYQTLFYQAYRRLHSDNTAAFLSSNSFDKVLTADPHDKDGNPVYSTKE
                     RQTWGIEDLEFYNRFLKYAETKLSDEKPFFAVLMTIVNHRNFNNYPEEKWHMYKNPRT
                     IREHYHNSVHNSDKALGSFIDSIRKSEKLKNSIILISGDHSYPLGDHGIVGNENGYYE
                     ESFRIPFLVLHKGNIKPVTLDKTAFSQLDIAPSLCDLLGIENESNAFQGISFFSFPEK
                     THPIPLIQPYSGRYLSIVVHPWKYIYHHRTGREYLFNLDDDPEEKNDLSSKKAYNQKK
                     EDLRKHLKTIYINQTLLEKNIMVRSSN"
BASE COUNT         5460 a         3498 c         4240 g         6452 t
ORIGIN      
        1 cgtactggta atattatcaa gtgctacaca acagattgga acgataccga aaattatgta
       61 gccgcatccg agataactat tgaccttgat tctgatctcg atcttgccaa gtttaagggg
      121 gcaatgcctt acggttacgt ctccttcagt caaccgaata gtacttatga agatataaat
      181 atcgaaggcg gtcttgatgc cagtacaatg tctcttatta actggggcga gggtggcact
      241 attgaggttt ggcgttacga cgaaatgcag agccaatggg ttcttgatga gagcagtacc
      301 ctacaggatg tctatggtta tgtgcgtgaa gttgttaatc ctgtaaccgg tgctcgctat
      361 cttatcaaac aaaacagtgt cgaggttatt gaagagtaaa tacagtctaa taaattataa
      421 aattgtaata ttatttccgc ggcaatgccg cggatttttt ttgcttttag aaaactttga
      481 taaattatta gagaagactt tttatgtatt gacgctgtag taaggtaaat ttttatacgg
      541 ctatcataaa ttgtgaccga ccgtggaggc gccatgaaaa aagaagtgaa gatctatttt
      601 gtaatacttg ctctaacggc tctcggtctg gctctgagcc acgatctttt ttcgaattac
      661 ttcaaagatg cttataatgt tacaactctt cagcggggaa gtatcgagct tcccagagag
      721 ttcccgggtg tgctatgttt tctgttagtc gcgatgcttt cttttattag tgatttaaaa
      781 attgccttta tcgcgcaaat ccttgctttt gccggtgtct ttgtgcttgg ttttgttacc
      841 ccctcgtttt tatggatgct cttttttctt tttatcaatt caatgggcat gcatctcttt
      901 ttaccgcttc aggacagtat tgccctctcg cttgtggaca agaaccgtgt tggtaagcga
      961 atggggcagt ttaagggaac catgacagcg tttcagatga ttggagcatt gattgccttt
     1021 gtggggttta aaagtggtgt tttttcattt accggaaaga taaagcatat ctttattatc
     1081 tccggtttta tttttcttgt cgtggcagtt ctttttcttg ttttggacag gatgataaat
     1141 cacaagggaa ttcatcatgt aaagacacgt ttcgttgtgc gaaaagagta cagtctgtat
     1201 tatgtgcttg ttgtgatgtt tggtgtacaa aaacagatga tgatggttta tgctccatgg
     1261 gttctgatag aacttctgca caagaaagcc gatactatat cgatgctcta tatggctggc
     1321 tcctttgcag gaatattttt tataccggct cttggcagat gggtcgaccg ttttggtgtg
     1381 cgaaagcttc tattcgcgga tgccatctcg tttattggtg tatactttgc ttatggtgct
     1441 cttgttatgg gtttcaccgg tggttttttt gctatggcag gattacctgt tttggcagct
     1501 tatctgctct ttattgtaga ccgtatgtct acacaaatgg cgattatacg tgttgtttat
     1561 cttaagcaga tcgctttaac cgaaaaagac attacaccta ccatctcttt aggaatcagt
     1621 gtcgatcatg tcgtttcgat cagctgtgct gtaattgcag ggtgggtgtg gacacaattt
     1681 ggcgcacact atcttttttt tggtgttggc gcactctcct ttgtaaacct ttgggttgcg
     1741 tttcgagtaa aagagagtcg taaatagatt tgttgacaga gtaataatta tatgtttttt
     1801 tgtatgaaaa aatgtaaaag gtaattatat gaaaaagata ctgtctttgc ttcttgtttt
     1861 tttatttgtt ggctgtaatg attcaggcaa taaggataaa ccttccgaca tctcgactga
     1921 taacatttct ttagttgata atggatcata ttatcaggtt gttctggatt atcgggataa
     1981 aacaccttat gaaatggggc agcagtatgg tactgttatt tcttcgaaag ttgagggcta
     2041 tgacaaactt attgatccgt atctgattga tctggcaaat aactcgggta tggacttttc
     2101 gaatagtgta atcttgtcta ttgtcagcaa aagaattaat gatattaaaa cacaaatacc
     2161 taaagaatat atggatgaat tggatggtct tgcttcggta ctggtgaata gtgattcgat
     2221 ggtaatcgga gatggaaaac tttccaaaga agaattttta ttagtaagcc ttctaccgga
     2281 tgttgtaaga ggtaccgctt gctccgctct gtcagttttt ggagaccgtt ctgaaagcgg
     2341 aagtccagtt gtcgcaaggg ttcttgactg gtttggaggc aacagtgatc agcttgttaa
     2401 aattcaggca gttgtggtat ataaaaaaag cacaggctct gtatgcacag taggctatct
     2461 cggatttatg ggggtaatca cagggtttaa tgacaaaggg gtttttgctg gtattcttga
     2521 ttcacctacg ggggcaactt ataattctga aggtaagtat tcgtatccat ttgatataag
     2581 atatgcctta gaaaacaacg attcactgca aacgacagct gactacttgt caaaacaccc
     2641 atacacgtat tcacatcttg tgtttttggc ggataaagag tcttccaaag ttcttgaaaa
     2701 caatatatca gatgatttta gacagctaag ggaagatatg tctccgctaa atggaggcat
     2761 tgtttggggg cttgaaaata cagttgctgc cgtaaactcg tttttactaa aagataattt
     2821 tgataatcat actgtttaca caggtaatta caatagatgg gaatacttct acaatcaaat
     2881 ttcagctttg gctggtcaga agcttgattc agaagatcta aaaaccttga taacatatag
     2941 aagtgatagc ggagctttgc atcaatatgg atcgatatat gttaccagca cccaacagat
     3001 gattcttttt tctcccgaaa gttttaaact ggaggtcttt tttagaccag aaggggggtt
     3061 gccagaaaca ccggttttcg aagatattgc tgtgacctgg taaattaact catcaattaa
     3121 gcttaaaacg gattgtctgt gttatcttaa cggacgatcc gttaaacggt gattctcctt
     3181 caaagaatat ccatcccttt gcggcatcaa tagctgcacg gtccaaggat gggtaccccg
     3241 agctttttat taccgaaaca tggccgctct tgccgtcaga cgtaatttct atttcgagta
     3301 tgacttctcc ttcgtgaccg aatcttcgag cactgtatgg gtagttgggc gggttgttgt
     3361 gtgcaataat atcggcaagt ctggttaggg ctgacaagtc agaattattt ttttttgaac
     3421 cgggtttttt tgttttaata tcttcgttta tttcaagtgt ttcatttttt ataaaaaaag
     3481 atgcttcttt gtttgaaaat attacagtaa agttgctctt gttcttttta tcaatggttt
     3541 cggtaggttt ttgttcagta aattcagatg aattgctaat aatattgact gtaacctctt
     3601 gtaagctgtt ctgttgtttg atgctgggct ttgtaaaaaa gttggtatca ctataatatt
     3661 ttgctatacc aaagacagct aaatgagcga taacacttgc gtataacgac acaagaaagc
     3721 ggttcatctt atgttcctgc caggggaaat attaaaatcg accatcactg gcggaagtcc
     3781 ggtgttaaga ggtttgccgt caaaaagatg tttgggatct tcactaatat caattatatg
     3841 tatgccgggg tttgttgcgg ttcggtcgct gacaaataga agatttgccg tatggagtga
     3901 taaagaacac aaatttccgt ttgttccttt gtttgacaag atagttttcg ataccgactc
     3961 attttgcaga tcatagagca aaaggtttga tctaaaatca ctacttgagg tgaggatata
     4021 cagtttctga tccggtccaa actgaaagtc ggtgatctct gtgttaagat cagattctga
     4081 aaccagatat cccggaaggc attcaagggt ctccggatct atcgcgacga ctcctccatc
     4141 ctccttgtaa aaatagccga actctcccgg gcaagatatg tagatcaatt ctttaccgct
     4201 gttgttaatg taatcgggag caattttgaa tcgcgaatag ggattaagtg cgttaactgt
     4261 ttcgtcgttt tctgtccagc tgagtcggat ttctttgatt acgatgtctt gttcggcatc
     4321 aataacaaca acactgctat aattgctgac tttaaatgaa gaatcaaggc gctgaagggc
     4381 aacgaaaagc aggttttcgt ggacatgcat catacacatg tgaggatgat tcattccggc
     4441 agccgagtat gatgacaggt ctatcgaacc actttttttg ccagtttcgg gattaacaac
     4501 aagcagctta gaatttccat aaacagaaac ataggcttta ttttttcccg atgtatcacg
     4561 gtaaatggct atatcctgtg ggttggaaga ggtgccggta gaaaattctg ttactatctc
     4621 gtgattcttt ttagggtcga gcacttgtat actgtccctg ccagcccggt tgattatata
     4681 tacaatattg ttaaatattc taactgacgc gtcggcgtga acatagccgg gagttgggtt
     4741 ttcaaatgtt ttgtaagaat tcaaatcaat aagagagaaa gaaccggttg tataatcgct
     4801 tgtaagcaca aatgcgtaat caattatagg taattctgtt tttgatggtt tatcatcgtc
     4861 gcaggctgct agcagcacta tcaataatag tgctgctata tatgttgaaa ttattttcat
     4921 aaggttattt tctccttgtg aatcttgcag aatttaaaac agatattcgg cggtcaggta
     4981 aaaagcgcgc cccggtaaag gataacctat cgtgtctcgg ctttttttat caagcagatt
     5041 gttaatttct gcaattaaag acaggtcttt gagtggtcta atctcccaaa gtaaattgtg
     5101 ataaatacgc ctgtcaatat aataaaaatt actgttggca cggtcaagat agttgctgcc
     5161 aattacatct attgagtatt gcagctcgtt attttgaaaa atcaatctgg ataaaccgtt
     5221 aaattgatga gttggtctgc ctggaagttg attgccttta taatatggta tatcactttg
     5281 gtcttctgta tgctggaaag tgtattcgcc gcttagtttt aatctttttt tgactaagac
     5341 ttcaccgcta acttcggtgc cataaattaa tgcttttccg atattttggg ccaacattgt
     5401 gctttgagaa ttctgtataa aaagaatcgt atcgtcaatt gtgttataaa aaaacagtgt
     5461 gctaaacttg atgacaggta atactttatt ttctgatgat gataagctaa taccggcttc
     5521 cctgttaaat gcattttcat ccgcaagatc gggatttcct agaacgccgt tttcatttcc
     5581 gaatagctcc ataaaagaag ggtgtcttcc ggtttgcgaa aaagaggcaa aaaacgcaag
     5641 gcttgttctt tttcggtcaa tgatgtttaa acgtgcggaa gtactgtaat gtagaaggta
     5701 gcgattgttg gtgtcttttt cgggactatt gtcaagaacc gttctttgtg tgtaaaaatt
     5761 gtcgtgtgcg acagttatgc gacatgcagg caaaacggaa attcttccat caaaaaaaga
     5821 aaattcgttt ccgatgccgc ctgcatagtt atcccttttt tgtgtggggt aggttgttga
     5881 atcatcataa taatcttcgg ttttttttgt gaatgtttca cggctgtacg aggccgttac
     5941 atcaaaaatg ctgttaagtt tatcaatgac ggaattaaaa tagagagcac agcctgctgt
     6001 gtgaaaggta ccggtctctt ttttattggt catcatgatc tcgttttcgg gatctgacaa
     6061 aatttgctct cgataagagt agttaatttt tgcgtccagc ctgttgcttg ttgagccaaa
     6121 atggttaaag tcgaaggccg ctgatgaatc atattttttt gatgtaaagc tgctatcaag
     6181 agccgtgtta ctgttaattc cggatactcc gttttttttg tacataaatc cgttattcaa
     6241 tgatagtgaa aaagaatcga aattatattt accgttaagg ttcaccttgc cggcaaagta
     6301 ttggctgttt tcccgttttt ctgttttgtc atcttcggtg ttaattactg gggttccatt
     6361 gtcattatga aaataaaaat catttttaca cgaatcgtat gctgtgaaga gaagataaga
     6421 atattttttt gtactggata atcttttgaa agagagatga taggtgtcaa aagaaccaat
     6481 agcagttttg atgcggtttt cagatttgcc gttttgaaaa atatttgccg ataaattttc
     6541 tacttccgag ctgtcagtaa caatattaat aacaccgctc atacctgttt taccaaaacg
     6601 cagtggagtt atgcccttgt atatttcgat tcgcttgatt gatgctacta atattgagtc
     6661 gatgtcaatt tcgccatgaa gggggtcagt aaccggaatg ccgtccacac atattagaat
     6721 ctggttatag ttgctgcctc ttatcgagac catgctgcgt gagcctgctc cgccagtgcg
     6781 gttaattttt atacccgcga cccggtttaa aagttgagac accgatgacg attgcacagc
     6841 ggatgagtcg ggaacaatta ttgtaaccgg attaacacct tgtgccagct tttccgtttc
     6901 ggtaagcctg ggagcgaaaa cagtaattgc attattctct tcttgcgcgc aaagtattgt
     6961 acagcaacaa aattcaaaga tgataatgat agttataaac ggttgccaaa aattagagtt
     7021 cactattagc tttgccaact gcgtcaagat caaaaccgac agatcccatg gttggataag
     7081 ggtcgtatat cgggtttcca aaggagtcga aatcatttcc gtctccggtt atatcaacaa
     7141 ttctgataaa tcgtatttca ttaatatcta ctcgattatc gattactgct ctatgatacg
     7201 agagctcgga aagatcaaag gggttccata tccttggcga tatttgccgg caagcccatg
     7261 tatcagggtt ggatcaatag tgccgtaacc gtctactggt tcttctgtca agcttatgct
     7321 gtcgaaacga acatagtctt tgccgttaga cgaaacctct acccatgcaa gttcaagaaa
     7381 ataattgcta aaagaatttt caaataccgc aaaatcgata ccagtgccgt ttgtgatggg
     7441 ctggttaaat gttaaaacta tggatccgtt gttgccaagt gaaactatat cggtgcttgt
     7501 tccggatgct ttgccggttg ccaagacggg gtccttccat tcgtcgctta catctttact
     7561 tggcaaatag tccttatagc ctgttgccca accggtcaaa acggaagagt ccatatgtac
     7621 agcgttgttg tttggcgtat cggcggctcc aaaaaaacta tccaatacgg tatagtttcg
     7681 gcggtaaata tcgctatctg cgcaatcggc tgatggtcgc gcagcaagat aaaaggtgcc
     7741 ggtttcgtta aacgtaaaat cattggttga ttgccatact ggtgtagctt ccgcatcttg
     7801 agtaacggat atttcgatat tgtcactaag gcttttaaaa gtaatctttg taccagttgt
     7861 aataattgtt ccacagtaat cgggtaaagg taacattgtg atggcttctg gagaaagaga
     7921 cccgtccgca gaggagccgt cacagagggt ggcggcgggt gctgtttcag tttcgcttgg
     7981 tgattctttt ttgtctgatg ccgtgcccga atcacagccg gttgaactgg aaacataaag
     8041 agttaatatt actataggaa gaaagtttct catgaattga tccatatgct aatctgtaat
     8101 aaacagattt tctttttatt cgaaaaatta tcgattgtct gaattaaaag agagcttgca
     8161 aagctgtcaa gtaagttagc cctgtacttt tttaaattat tctgttgttg acaaaagaaa
     8221 tagtcattag ctaaaattat tatcaggaaa cttcgaaaaa ttacaatgtt ccggagggtt
     8281 acatggttta agtgcctatt ctaaaggaat ttttcgcaag ttccttgtta taaagttaat
     8341 taatagaggt tgtcatcatt aattgcagat cttaataaag tttgcctacg aggatcatat
     8401 gaagtacaga tcggttttac tattgtttct tctgtcgtcg ggatttataa tgtcgtgttc
     8461 tagattgcca caggataatt cggatgccaa tataatttat gtaagtatct tgccccagaa
     8521 gtattttgct gaccggattt gcggggataa atttgatgtc cgggtaatgg ttcaaccagg
     8581 aaagagccca gccacatatg agcctttacc tgcacagatt acagaattat ccaaagccaa
     8641 gatgttcttt gccataggag tccctttcga aaactccttt ttgcctaaaa ttcgtagtgc
     8701 tataccagcc ttggcaataa tagatacatc atgtagcatt gccaaacgga gactagaaga
     8761 tcatgaccat catgatgacc atgatcctca tgaaaaaaac atagctggtg atcttaaaat
     8821 aacaggtgat tcttgcaatt ctatcaagag tgatataaaa aagcatgatg ccaagcaaga
     8881 tctggttaag aggtcaaatc atggcaagtc ggccgaagat ccgcatgtct ggatgtcccc
     8941 agtattggca actgttgttg cacaaaccat gctagacgag ataataaaaa ttgatcccgg
     9001 taacgaggac tactataaac agaacttttt gctgcttgaa gccgatcttg aatctcttca
     9061 taaagagtta actgaaagtc taaaaagtct taagggaaaa acaatgctgg tttttcatcc
     9121 atcatttgga tatttcgctg acgagtataa gctaaaacag gtggctatcg aagcaggtgg
     9181 aaaagaggtc gagcccgcgg gacttgtcaa gataatcgag ttcgcgaaaa aggagaaagt
     9241 gtccatgctt tttgtgcaac cagagttttc gcagaggagt gctcgtgccg tagcagatgc
     9301 ggttggtgcc aaagttgttg tcgcatcgcc tcttgattac gagtatatca cgaatatgag
     9361 aaaaattgcg atccgattaa gagagttaaa atgacagaaa acaacattat agagataaaa
     9421 gatctatctt ttgcatacgg caacgaaagt attctttcac atattaattg tgaaattaaa
     9481 gataaagagt tggtttcgat agttgggcct aatggaggcg gaaaaaccac tctagtcaga
     9541 ttgatggcag ggctactcta tccggttaaa ggcaaggttt atattgaagg ggtatccaca
     9601 accgagaggc atccgatagt ggggtatgtt tcgcaaaaca gcatgatcga tgacaagttt
     9661 ccggtaacgg ttcaggacat agtactaagc ggaacaatga aatccggctt tggattttat
     9721 aataaaaatg atcgtaaaaa ggcaaatagt gctcttgaaa gcgttggtat gagtaatttt
     9781 gtaaaaaaac ggcacagtga tctttcgggt ggccagcgta agcgagtgat aatagcgaga
     9841 gcacttgctg gtggagcaaa aattctgctt cttgatgaac caacggctaa tcttgatgcc
     9901 aattcttctg ctgatctgat agaattaatc aaagacctta acaaaaccat aactgttgta
     9961 ctggtgactc acgattacgg atttgtttct gatattacca accgtgtttt ttgtataaat
    10021 aatcatcttc acgagcatcc attaaaagag gccgaagagc gtttcgcaat gaaccttaaa
    10081 cgggtcgatc acacagttgt actcaataaa tgtaaaggtt gcgagggaaa acaatgagtt
    10141 catttatagc agcgcttttg aatcctgata tggtttttat acgttatgcc ttgtttgccg
    10201 gactaatatc tgcggtacca tttggaataa ctggaagctt catcgttgta aagcgtatag
    10261 ggtatgtggc tggagccata agtcatgctg ttcttgccgg tatcggtttt gcaatgtatg
    10321 taaatgcaaa gtatgataaa gaggtgttaa gcccaatgct tggtgcgttt atcgcggccg
    10381 tgactgccgg aattattatc tcttttgtgt ctcgttatgc caaagaacgg gaagatacga
    10441 ttatcggaac agtttgggca ttcggtatgg ctattgggct tctgtttata cacagtacac
    10501 cgacttatat tgatccaatg agctatcttt ttgggaacat ccagctttta ggatcgcttg
    10561 agcttaaatt gataattatt cttgctgtta ttgttgtctt tgtggtggtt ctgttttata
    10621 accagcttct tcttgtgtct tatgatccgg aatttgcggc tttaagagga ctcaatgtct
    10681 atttttatca ggtcatgctt ataatgctta tttcgtgtac agtagttctt atggcaagtt
    10741 ctgttggtat tgtaatggtt attgccttgt tgaccattcc gtcgggaata gcctcatttt
    10801 ttacaatcaa gctttggcac atgataattg tttcgtcaat agtttgtgcg gtctctgtag
    10861 tgtctggatt ggcattcagt tatatgttag cgatgccgtc tggtgcggta attatcgtag
    10921 tgtgcggaat aatctacttt gcggctatga tgctaaacgc catattcggt attattcaaa
    10981 aaaacagagc cgttggtaag cgtgatcaaa taaaaccgta attcaagatc tcttccaggt
    11041 gtttagttct ggatgtgcct accagtacac gtgatatttt tttattgttg gtcacaaact
    11101 taagggcttc tttgacagtg ttgtcaccaa ggctgtttct tttttttagg cattgggtga
    11161 aattcgtacc ttttgtatca tccggtttag tgcagatgtt gccattaata tcaaacagag
    11221 ggttaagcct gcgaatatcg tttttggcaa aacggtactc ctcgtttact gctggcgtaa
    11281 gtaagccttg cgcgagaggg ctgtaggcga tgaagtctat cctgttatca taacagaaat
    11341 cgattaactc ttcagattcc tgctgcagaa aattgtaccg gttttggctt gagcagacag
    11401 gtccaagttt caaggtctct tcaagtctgg gaatatcaaa gttacagact ccaatttttt
    11461 taaccatacc ttcgtctaaa agccgggtca gggctttgaa gctctcgtca agcggggttt
    11521 gcgggtctgg ccagtgcaac tggtacagct cgatgctctc tctgtttaat cttttcagtg
    11581 attcttcaca atcgcgcagt ataaaatcgt aagaaagatc gtgagttacg cggccgttat
    11641 cgattttaag cccgcatttt gtggctacaa caattttgtt tgatatgctg gaaggagttc
    11701 tatcaagagc ctcgcccagt aatttctctg aaagcccaaa accgtagacg ggtgcggtgt
    11761 caaaaaaaac aacaccaagc tcgatcgctc tgataatcaa attaattgcc gattcagatg
    11821 tggctccttt ccagcctttg ccgcaaagag gccaacatcc gagtccaaaa cgcgggtggc
    11881 tattcaaatt tctcaagaat tcgttcaaag tcatcaagag agtagtagtt tatttcgata
    11941 cggcctttac cggtggagga gtgttttatt tcgacctttg ttcccagagt cgagaccagc
    12001 ttctcttcca tttttctgat atgtggatct ttgtgcggct tcttggcttt tgattcgcct
    12061 gaaagcttat cctgatcagc cttgatgatg ttttctaggg ctcgcacact caaattttga
    12121 gaaagaatct gattgaagta gtactctttc tgactgcccg gtacgccaag cagaactttt
    12181 gcgtgcccct ggctaatctg tccggcagat atggcgttga ttatgatatc gggtaacgag
    12241 agtagtcgca gtgagttagc gattgtagca cgctctttac cgacgcgttt ggctacatcg
    12301 ttctgtttaa gattaaaacg gttgatcagc accttgtatg ccttggcttc ttctatcggg
    12361 tcaaggtctt gacgttgaat attttcaatc aaagctattg ttacgttttc ttcttcggtg
    12421 gcttttacaa ctattgccct tatggtatcc atctccagaa cttttgccgc tctgaaacgg
    12481 cgttccccag caataataat atatttgtca cccgatttac gcagtaaaac cggctgaatt
    12541 aagccaaccg atcgtatcga ctcggctagg ttttcaatct caatttcatc aaaatgctcg
    12601 cggggctggt ctgggttggg agttatgttg tctagaggaa tttcgacaac agattcaccg
    12661 gcaacatgcg cgatagtctc ttcaacatga tcagcagctc ttgtcggtgg agcagtcgtt
    12721 atgattgctc ccagaccttt tccgagtcgt ttagccatta ttgattacct cttcggcaag
    12781 tttttcgtat gtttcacttc ccggacaacc cgagtcgtaa acaccaatag gttgtccaaa
    12841 agatggagcc tctgacagct tgacgtttcg gggaataatc gtttcaaaga ccttatcctt
    12901 gaaatattct ttaacgtctg ttacaacatg ttgcgacagt ttggtacgtg aatcgtacat
    12961 agttagcaca acgccttcta tgcccagctg cttattgata ttttcttgta ccatcgaaat
    13021 aattctaaga agctgactta aaccttccaa ggcataatat tcgcactgaa gcggtataag
    13081 aacagtatcg gccgcgacca aagcattaag tgttaaaagg ccaagagatg gagggcaatc
    13141 gattagaaca tagtcataaa agtcggcaat tttttttaca gcttctttga gtttaaattc
    13201 ttttccttcc agatccatca tgtctatctg tgctccggaa agatgaatat ttgcaggaac
    13261 aatatcaaga tttggatatt ctgtattgaa tatagcttct tctatgccta tctcaccaat
    13321 aagcacttcg tagattgttg tctgaagctc ttcagcatta accgaaacac cgaacccggc
    13381 gtttccctgc ggatcaatat caataattaa tactttttta ccttttgctc ccagaaaagc
    13441 cgcaagattt acggttgttg tggttttacc cacaccgcct ttctggtttg aaactgatat
    13501 tactcttccc atggtataac ctcttttgcg gggattgttt gcttattgtt ttatcagata
    13561 gcttcaattg agcgtctgat ctctgtgcgt ctatctaaat cgtgttctag tgacagaaaa
    13621 cggcattcgt aagccatttg atagcagaat gacagcatac acttaaaaag accgtccctt
    13681 actgaatcac tcatgatata ttttccgaca atggttctag cgagcggtgg aagtttatcc
    13741 tgtttggcaa tcttcatatc gtaaaacgga agaagaaaac catcatcctc atggtcatga
    13801 acaacacccg gtcgcaaagg ctcttttgat gactcaggcc ttgttgaagg atgtagctcc
    13861 ataaaacgct gatgagcacg gtaaaccgca tgaagctcaa cagcgaaagc ataaaaaact
    13921 gcccccatat ggtcaacaaa agattttata tgagtcatag ttgccagttt tccctgctga
    13981 atcttgcgta cctcgtcaag cgatataagc gaattagggt ttttggtgcg ataagcgaac
    14041 agatcacgct gaatatccag cagcatggat attttagact caaaatagcc gggtgtaaaa
    14101 atggcgcctt caaatgtggt ctgatcggaa gatacaaatt tttgtggtga tccgttcaaa
    14161 agcggcatat atacatgaac aaaatagttg gtacagtcat caagaaaaaa gaagaagttt
    14221 tcttccggat agtttccaaa ctcctggcgc ttattgctaa aaatatgcca gttgtcgcta
    14281 aaggctttgt atgttagttc ttgcccgtca attttgagat taataagttc gaccatctcg
    14341 tcatagggct ctagctcttt cttaagagct tccagactgg cttttcttaa tgattcgcta
    14401 tctttaccag cattttttaa tagctcacgg ctgcattccc agcgtgttgg gtcggcgtct
    14461 ggagctttta ttgaatagta gcgtttaagt tcggtacggc tcatttgacg tcggaagata
    14521 agtgtctcaa ccaatactac agttaggtcg cgtaaatttg caattttcgt tgcgttctta
    14581 agatagcgat ccaaagatcc ttccgtgttg gcgtcgggca tgaaagagcg caaagtaaca
    14641 attatatcgt aaacagcttt ttctttggca aggttacgtt tttgttttga tgaactttcc
    14701 ggatcattct ctgatatgat atctttcttg taatcaccct gttcacgcag cttggactca
    14761 aacattaaaa taatgttttc tcgtaattcg gtttctatca ttgaaaaact gaataccatt
    14821 cgtgcgtaag gaagcagttc ggcctcaaac gcagctgtgt caaaccagag acattggttg
    14881 tagcctgcgc ttaaaaaggg aatatttagc atccatttgc gtaacagcct aaggtgattg
    14941 aaaattattg gtgaggttat tgcaggatcg tttacaatat cttccataac ctggggaaga
    15001 acggcagcaa ttttttcctg atatgagtat atggtacgaa aaacacggaa tagtttgcgt
    15061 gaaggaacaa ttcgtttttt tatccagaat tttaaaaaat ttgatgattc gataagataa
    15121 cgttttttaa aaaggcctgt ctctttgtat gctttgagca gtgcgttaac atgataagat
    15181 ccggacgcgg gagagggaat aacagtagtt ttttttgata acttaaccgg tgtttccgtt
    15241 ctgcgtgaag cagatttggg taatatggtc gtctttggtt tggatgctgt aacagatttt
    15301 acagggcgtg gtttatcagg gcagtcctgt attagttgta taactgcgtt aaatttttcg
    15361 tgaggctgaa gacctgcgtt ttttagcaaa gcatttaccg cctccatttt ggagtcggga
    15421 atatccttgc gagcgatcat tcttttagtt ttttcttcaa gtcgcatatt gctttgatgc
    15481 atcaattact ctctcgtgcc acttttttag ccgattcttt ggtaaatttt tcattagaat
    15541 agtcattaat gtcaattatt aatcgaaagt atttataaca gcgcagaaac cttggtattt
    15601 ctctttttta ttggaattga atatatcgca aaatttgttg accgaaagct aagtctacat
    15661 tatcgtaagc gaacattata aaagtacagt atttgagtag gaacttggtt taatagttaa
    15721 ttgttagagg gtaccaaaaa ctatgtcaaa tttatttaga tgctcctcct gtggcagtcc
    15781 cgatgtgcgg ttaaataaac agaatcagta tcaatgttcg tattgcgggt cactttatgt
    15841 acataaagag cgtgcgtcgg tttatgccgc aaaagagata ggtaataata agagtaaaat
    15901 tcttctggtt tctgctgcca tattgtttat aacgcttttg agtatcgctg tctattatgt
    15961 gagcgttgag aatagggccg gaaaaaatca aactgtcaat attaatagtg tatcggtgaa
    16021 tcaggataag gaagatccga ataaaacgac agttgatggc tccgttgctc ggcaggatgt
    16081 actgactgat aatacacctg gtgctgccaa aaaagctgaa gctgaagtcg ggcctctcgc
    16141 agcagtcagg gattccatag gcggtatata ttttagcgga ttttatcaaa acattggcga
    16201 ggctgttatt acctatccta aggttactgt agtgcttttt gacaaatatg gtaaagaact
    16261 taaacgtgcg gcgggatatg gaaccagaag ctttttgctg ccgggtgaga aaacaatagt
    16321 cagagcatta gtatctggtg ctccgaacta tgataaaatt gaggtactct gtgaacctca
    16381 atcagcatca aaattcacaa ggttgtcgcg tattaaactg actattttag agtccaagat
    16441 gaatttttca ggccgaggac gccttgacat tacaggtaaa gtaaaaaata ttgacaacaa
    16501 agattgccga atggtcaaga tcgaagctct tgtttataaa gaaggaaagg tcatcggttc
    16561 cggacttgct catataactg atacaatacg ctcatccgaa acggcgactt tttcgatgta
    16621 cataccttca aagattgact ctgccgattc ttttgttctt gattggcaag gcacacctgt
    16681 caggtaatgt tgttatagcg gtgtacaaga taataacgtt ttataaagag ttatattacc
    16741 tgtattgaaa tgaagataat gaagattttt tggtaaagcg gtttgattct gtggagagaa
    16801 taatttgaat aatataaaac attggttaga taaaacttca gaaagaggat tggcgtatca
    16861 ttttttcctg tttttaatag tggctatttt gttaaaactg gtttaccttt ttgcctcacc
    16921 tatctggagg gtggatatct tcttttatat tcaaggtgcc aaaaccatgc ttgacggagg
    16981 tgttcttttt aaaaactttg tcgagattaa acccccaggt attttcttta tttattactg
    17041 gctaagtctt gtttttggat attcgaatat tttggtttca ataaaattgt tagctcttct
    17101 gtcacagagc ttgactgctt tgtttgtttc aacaactgcg tcacgtattt atacaaaaaa
    17161 aacaggtttt gcagtgggga tggcttttct tctatcaagc tctatatgtt atagtttttg
    17221 gcccccaaat attatgctta ttgcgcttac tccgttactt gtgtcaatct ggttatgttt
    17281 tttatacata caaaaaccta tgatcgctta tctctttttt actgggcttt gtatgggtat
    17341 ttcttctgtg ataagcacca atttgattct cttcactctt attgttccca tactttgttt
    17401 gatgagttat aggtccctta agaagtttat agccggatgc ttaatagcgt tcgcaggatt
    17461 tattgtagtc tggcttttgc tggcagtaca tttttatctg aataacgcgt taactgattg
    17521 gctatggtgg aatttcaaat gggcctctat atatggaggc acacgtccaa tatatttaag
    17581 gatttttaat tttttttggg gtatgatacg gtcatggcag attttacctc tatatgtgtt
    17641 atcattttat tctctgtata taattacaaa aaaaaggttg tatcagaatc atcaggatta
    17701 tttagttctt ggtatattgt tcgttatctc gctcttatca cgattggctt ttggtaaatc
    17761 tgaaccgcgt tactaccttt atatggttcc atttatggtt cttcttttgc ctttggcaat
    17821 aaaaggcttt aaaaaaattt tggtaaaaaa aacaactttt atcattcttg cttatatcat
    17881 aatcgcatta tcttacacta gttttgaagg tatatcaaaa ccttacagac ggattgttac
    17941 aagaaaagtc ttacatgaag cgataaaaga attctcagaa ccgactgata gaattttcgt
    18001 ttggagtgaa ggctatgaga tatatttaat gtctgaaagg tcaatggctg gcagtttttt
    18061 ttcgcccgcg cagcatcttg ctaacggata catctggatg cgtaacggat ttcgtaatat
    18121 tgatgttcca tggcaaaaat ttatgattga gttcgccacc gatcaaccgg aactgattat
    18181 tgatttgact ggaaatttta agggtgaagg cgaagagtat tatgataaat cattatttgg
    18241 ctatatgaaa agtttcatta aagaggtaaa cgaaaaatat tctatagtat cagagtttgg
    18301 aagtgccgtt atatggaaga agaaataatc gattttgatc acaattttat ggttttgtga
    18361 tatattgata ttgcgacatc ttgtataaaa ttaattgctt gaacgaacca ttatgttttt
    18421 ttcaagaaga gtctgattaa tatagatagt tttgagatgt ttccttaaat cttctttttt
    18481 ttgattatac gctttttttg atgacaaatc gtttttctct tcgggatcat cgtccagatt
    18541 aaatagatat tccctgccgg tacggtggtg gtagatgtat ttccatgggt gtactactat
    18601 tgacagatat cgaccgctgt agggttgtat taaaggaatg ggatgagttt tctcgggaaa
    18661 agagaaaaag gagatgccct gaaaggcgtt cgattcattt tctattccta gtagatcgca
    18721 tagtgatgga gcaatgtcaa gttgagagaa ggcggttttg tcaagagtta cgggttttat
    18781 attgcctttg tgtaaaacaa gaaacggtat tctaaaagat tcctcgtagt atccattttc
    18841 gttacctaca atgccgtgat caccaagcgg atatgagtga tcacctgaaa ttaatattat
    18901 ggaatttttt agtttttcgg atttgcggat tgaatcaatg aaagacccca gagctttatc
    18961 cgaattatgg acagagttat ggtaatgttc gcgaattgtg cgtgggtttt tgtacatgtg
    19021 ccatttttct tcggggtagt tgttaaaatt acggtgatta actatagtca tcagaactgc
    19081 aaaaaagggt ttttcgtcag ataacttggt ttcggcatat tttaaaaatc tattataaaa
    19141 ttctaaatct tcgattcccc atgtttgtct ctcttttgtt gagtaaactg gattgccgtc
    19201 tttatcgtgc ggatcagcag taagaacctt gtcaaaactg ttggaactta aaaaagctgc
    19261 cgtgttgtca gaatgtaaac ggcgataagc ctgataaaaa agggtttggt atccgttatt
    19321 ttttaaaaca gacgcaagag aaataaaatc gttatttttg taatgaacaa aaacctttcc
    19381 tttatacgaa ggaattcccg agaaaagagc cgcatattgg cccttgcaag tctgtataga
    19441 gttcccgtaa aaatgaggaa tatatagccc atcctttaca agcctattaa agtttggtgt
    19501 tatctcttta ccggattcag tatggctgtt aacgacagaa gcattaaaag actcgatcat
    19561 aataaaaata attgagggtc tgtctgtcga atgtcttttc aagtcgacgt tgtttgattt
    19621 tttaatgttg tgtttaaaga acgggtaggt
//
LOCUS       DAOTGZ010000022        19500 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_3064
            NODE_3362_length_19500_cov_5.867678, whole genome shotgun sequence.
ACCESSION   DAOTGZ010000022 DAOTGZ010000000
VERSION     DAOTGZ010000022.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868088
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 19500)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 19500)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:22:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,785
            CDSs (total)                      :: 2,751
            Genes (coding)                    :: 2,732
            CDSs (with protein)               :: 2,732
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 12 of 19
            Pseudo Genes (internal stop)      :: 5 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19500
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3362_length_19500_cov_5.867678"
                     /isolate="AMR_MDS_3064"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..143
                     /locus_tag="P5123_02810"
     CDS             <1..143
                     /locus_tag="P5123_02810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017322794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="tRNA (guanosine(37)-N1)-methyltransferase TrmD"
                     /protein_id="HRX15223.1"
                     /translation="HQVPSVLLSGNHAEIAKWRLNKRVEKTRRVRPDLYKQYEIIKEK
                     QR"
     gene            149..508
                     /gene="rplS"
                     /locus_tag="P5123_02815"
     CDS             149..508
                     /gene="rplS"
                     /locus_tag="P5123_02815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002761126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L19"
                     /protein_id="HRX15224.1"
                     /translation="MNAVEKLGLELSEVKREVDFEVGDTVRVHYKIIEGTRERIQIYE
                     GAVIAINNKCAGKTFTVRRVSFDVGVERVFPLLSPRIAKIEVIRKAKVRRSKLYYLRE
                     RTGKSAKLKERRHISKK"
     gene            516..1142
                     /locus_tag="P5123_02820"
     CDS             516..1142
                     /locus_tag="P5123_02820"
                     /EC_number="3.1.26.4"
                     /inference="COORDINATES: protein motif:HMM:NF000595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004523 - RNA-DNA hybrid ribonuclease
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HII"
                     /protein_id="HRX15225.1"
                     /translation="MTAESPSFEYEQIYSKGGPIAGLDEAGRGALAGPLYVGCVVFPP
                     SYYLNPPSEILVINDSKKIPKKRRLQVYQIVIDHALFYDFEPSCPDEIDTYNINGATR
                     LAVNRLLDRVPFEICTAILDGNFRFASKHPLVSLKKGDSLSLSVAAASIIAKVQRDSF
                     MSDLKSEKYPYGFDKNVGYGTRFHLDAIGKYGPSDQHRRSYEPVRSLF"
     gene            1139..2101
                     /locus_tag="P5123_02825"
     CDS             1139..2101
                     /locus_tag="P5123_02825"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRX15226.1"
                     /translation="MKIENAIKIEGALSRNRALTSLSPGDSVRVVILEKIDNARAVVN
                     LLGNKVTAQFSEGVPNEKTVLLTITGNSGGKLVFKRQTGSSEAMTFKSVLLGDKIPDQ
                     LFLRSIGAGVSDIFTFNRLLLQHRLKRRITDHKKIFSRLLSQGFSEKELSSLSIFGLK
                     KMPSIITHFSDLFVENSVSDSELEELETKLGNLSNEDLQMLFDSVFDESSALFLLNID
                     STYRWFNILFEEDYICISFEFEKSGLIQLVAKEDTTDINISLFFESGDFRKMFMSEFE
                     NLDKIIRNSNKKIRLVTWLLSDSVTMLNKEINRAVQNPVLDIRA"
     gene            2098..2361
                     /locus_tag="P5123_02830"
     CDS             2098..2361
                     /locus_tag="P5123_02830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013135737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="EscU/YscU/HrcU family type III secretion system
                     export apparatus switch protein"
                     /protein_id="HRX15227.1"
                     /translation="MINKSKKSIALKYDAKIDAAPYVVVKACNELSDIMLKIAREKGI
                     PEYKDDDLAEALYVLPEGQQISPDLYKAVAEVIAYCYSIKGMD"
     gene            2368..3531
                     /locus_tag="P5123_02835"
     CDS             2368..3531
                     /locus_tag="P5123_02835"
                     /EC_number="3.1.4.-"
                     /inference="COORDINATES: protein motif:HMM:NF014069.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HD-GYP domain-containing protein"
                     /protein_id="HRX15228.1"
                     /translation="MNRILVEELKPGMRFTKEVMIDSSNVLAAANVPIQESDIKRLMK
                     WGISEVTTEGQLLTGAAGGGSVAISEQFNKVLDDYNVLISLKDQLIEVHTRTVKEVSR
                     IHTAIRNDRMFNIQELTKCVEDIYNLIRQNRNVFLFVQRQENEKDTMAVHSVNSAIYA
                     MVIGLTLEYSRSKVKDLGIGVLLINAGMVQIPLYIVNKEGDLNERELQQIKSHPVLGY
                     QVLKNLGNISEDIAQISLQHHEQFNGGGYPKRLKGIEISEAARIASVADNYEAMVEKK
                     SYRPRHFYYTAMKQLVATGARKFDPVILRMFISILSVYPIGSLVELNNKKIGVVIGTI
                     KDNPLRPIVKMVRDEKGERVKGVEIINLVEEFDLFITQTIDEYKAGINLSELF"
     gene            3531..3884
                     /locus_tag="P5123_02840"
     CDS             3531..3884
                     /locus_tag="P5123_02840"
                     /inference="COORDINATES: protein motif:HMM:NF009150.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004518 - nuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YraN family protein"
                     /protein_id="HRX15229.1"
                     /translation="MDDRADKGGRGEQLAVDYLENQGFTILTQNYRYGRYAEIDIIAQ
                     KDDLLLFVEVRSQDTFQMGSALHSVRQSKIDKLRKCARSYLAFVYPQDREVDCRFDLI
                     TIDKGELTWHQDISR"
     gene            4185..4352
                     /locus_tag="P5123_02845"
     CDS             4185..4352
                     /locus_tag="P5123_02845"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRX15230.1"
                     /translation="MKNLMLKEGREIESGLGVERSSTRVDKYKQLISDESYIDFAITR
                     IATDLSHYLTK"
     gene            4571..7318
                     /locus_tag="P5123_02850"
     CDS             4571..7318
                     /locus_tag="P5123_02850"
                     /inference="COORDINATES: protein motif:HMM:NF012602.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-dependent oxidoreductase"
                     /protein_id="HRX15231.1"
                     /translation="MPVTRRNFLKIAIGLLGMAAAGGITLFEYTRRIPLFKKRSNSIR
                     SFCPFCSLNCAVKIYYTDTKITSVQPEKKSFESNGRICPKAHMLREMFNRPDIISFPL
                     VREAGSDVFLKTDYSDVLNRCAETINRTREETFITESSGFKINSFSGAAVIAGDKIGC
                     EDAYVINKFSRVIGISRVGSGALIRHNSSVMALCSTFGIAAQHNPVYDIVNSDVVFII
                     GANPARTNTMQLCSIQEARKRGATIIVADPIAGETAALADIYLPLLPGTDTILLCGLI
                     RYLLHNNRYDAEYLRDNTDASFLVNESFLVNADGTFSGYNAESAYDTLTWSYIIDERG
                     NPRTDSSMKKINCVFQILKKSYSAYTPDNVLNMTGLGADLFLKAAETLASVSLPAKSC
                     SVIFGGGFTQSSGGQGVRAVSILQLLCGNTGIAGGGIYGINDRSNQQGVSDQIGNWNC
                     LPGYLPIPDSSKMENDYESYIKNNSKKSNDFSVSNYWNNFQGYFESQMRCWFKSISPE
                     KAFSILPRGSSNYSFSKLLDDVGKGSIKGLIVFDEDIFAAPVSENRIINALNKLSWII
                     MFVSNQNRLSRLIGKLSFSKNASVILVPLPSYINSEMHFVSSDRRFNAYDGSAVFKRG
                     AAMYPQIAFALQGIAKKIAEIHKANGGVFSDPIIDSEWRYSTIKSIAKELGGVNRANG
                     SSDNNIEQVFSESYVCGNWLYSDIIGDKRLLKRDKYSQNGLLRDTDRCWPSGEQVLFN
                     RAGVNFKDGSPRHGKCIVFEDVKKIKNEELARKRPFRFTRDGVAYLFAREMISGPLPI
                     SGLEIKDDNNDFLLFTSSWSESSFAEMFNYYNEYYPEDCILMNRESAVTAGFENGKNV
                     VVQSSVAKITVPLYITERIANKRAVNGIGVVFKELLDNIDSDIITPVTVRIYKEISNG
                     R"
     gene            7308..8084
                     /locus_tag="P5123_02855"
     CDS             7308..8084
                     /locus_tag="P5123_02855"
                     /inference="COORDINATES: protein motif:HMM:NF024644.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S dicluster domain-containing protein"
                     /protein_id="HRX15232.1"
                     /translation="MAGEKCILIDSSKCCGCGSCYFSCKSWNSIPASGGSRITDGEFS
                     VIRDMRPQNWLIVKRSADDGRLRAWPDLCRHCSDALCIKHCPVEAISNQDGWVTINRD
                     ICIGCGACEVVCPYGAISVYRGDSKDVLKKNRAYKCDGCSENYSEKPACVVSCPTGAL
                     EYGNRSRLIRKAMRRVKKLKKEYPETKLYGINEYKGQNVLFINMKTVEHENNDEQLGY
                     NLLQVKTLYKTVSMFTRSFSVMSGHIYGFLHFIVTGKKNV"
     gene            8287..8733
                     /locus_tag="P5123_02860"
     CDS             8287..8733
                     /locus_tag="P5123_02860"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRX15233.1"
                     /translation="MKKIEVLIFVVVFLSVSSCSTLQSFFKSEMTVAQAIMAKDRMDK
                     ITNPAQKDIMYKDLQNKIIVVPRAKVLDIIESINVDYSYAVIARVETEKGPVDLYLYS
                     KDQKLIASLSKESSEISAKGEFSKFFQLLDDSYAKIELVKAKINKL"
     gene            complement(8820..10121)
                     /locus_tag="P5123_02865"
     CDS             complement(8820..10121)
                     /locus_tag="P5123_02865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015711986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylacetate--CoA ligase"
                     /protein_id="HRX15234.1"
                     /translation="MTSCFNDYEIQHLDRDALEALQLIRFKKIVKQALKTEFYKQRLG
                     SVGIKDQSDITHINDITKIPFTTKEDLRDTYPYGLLAVDKEEVVRMHASSGTTGKPTV
                     IFQTQTDLDRWTNLTVRSLLVTGCTKKDVFQNMMTYGLFTGGIGLHYGAEKLGMFVIP
                     ASSGNTKRQFMLMKDFQTTVVHATPSYLLHLFSEMESMGYSRKDFVIKKAMIGAEPHS
                     EEIRQKIEDYLQIDAYNSYGLSEMNGPSVAFECEYKKGMHLWEDSYIMEVVDKEKLTP
                     VPEGTTGELVLTNLIRESTPIIRYRTRDLTKIIPGECPCGRTHRRIERILGRTDDMLI
                     INGVNVFPSQIEEVIMKMPNIGNNYRIVVRKKGTLDELIVDTEVNSDIISDDTRKLYA
                     LRDQIRDELKASITISPQIEFHEPGHLPVQQGKAIRVIDER"
     gene            complement(10118..10684)
                     /locus_tag="P5123_02870"
     CDS             complement(10118..10684)
                     /locus_tag="P5123_02870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020260621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="indolepyruvate oxidoreductase subunit beta"
                     /protein_id="HRX15235.1"
                     /translation="MKYDIILCGVGGQGVLSVAAAIATAAGIENLNVRQSEVHGMAQR
                     GGAVLSHLRISDTEIHSDLVAEGSADMVLSMEPLESLRYISFLKPEGIVISAQEPFIN
                     IPNYPELDQIHTEIKKMKNSKLVDTAHIVKESQNPRASNMAIVGAASRYLPVKTESLK
                     KAVAILFKNKSEDIINKNIRAIELGGSI"
     gene            complement(10684..12288)
                     /locus_tag="P5123_02875"
     CDS             complement(10684..12288)
                     /locus_tag="P5123_02875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010258269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-dependent enzyme"
                     /protein_id="HRX15236.1"
                     /translation="METYALLGDEAIALGAVDAGLSAAYGYPGTPSTEIMEYLQQIEK
                     KEGKPLARWSANEKTAYEEALGASFYGKRTLVTMKHVGLNVAADAFMNSALLDIRGGL
                     VIAIADDPGMHSSQNEQDSRYYAEFAHIPCFEPVNQQQAYDVMSEAFDLSEKLHVPVM
                     VRLVTRLSHSRAAIKTSSRKKENTGVKAPDVKQWMLLPALARKQWHSLLEKEKEMKSI
                     SETTDWNPLVLKNSKIGVITSGLGKNYFLENLDQCSQEFNHLHIGYYPLPYDKINKIA
                     SLSETLIVIEEGEPLIEKQLRGILPLTIDVKGKLSGAIPREGELTPDTIRSVLGLSPK
                     ASAISSSMPLPGRPPQLCKGCPHDDTYTFIKEVVSELKESAVMSDIGCYALGALPPLQ
                     VPETIVCMGASITMAKGAAESGMKNVMAVLGDSTFFHSGLTGLVDCVADNAPVTILVL
                     DNSTVGMTGGQETILPSERIKQIALGVGVHPDHVHVVNAHKNDHDSNAALLRKELDFN
                     GVSLIIPVRECIEYFRKKRKAEKGDN"
     gene            complement(12288..13895)
                     /locus_tag="P5123_02880"
     CDS             complement(12288..13895)
                     /locus_tag="P5123_02880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012956584.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium:solute symporter family protein"
                     /protein_id="HRX15237.1"
                     /translation="MNYTVLIIIILIYLTVIAYLGYRGYTASKSSSDYMLAGRNTHPF
                     IMAMSYGATFISTSAIVGFGGVASLYGMSLMWLCFANIFVGIFIAFVFYGKRTRLMGL
                     NLDAHTFPELLGRRFDSRFIQLFAGLMIFIFMPIYAAAVLIGAARIIESFLGVPYELS
                     VLVFSVLVGGYVIMGGLRSVMYSDAIQGSLIFIGMVTLLIYTYTKLGGVFEAHSSLTQ
                     LANAIPENIKNSGVFSWTSSPQTGSSLWWVIYSSLVLGVGLGVLSQPQLAVRFMTVKS
                     NKELNRAVAIGALFIFATVGTTYLVGPLSNVFFFEKYGHIAFEAAGKNLDRVIPVFIG
                     ETMPIWFVYLFMMVILSAAMSTLSSQYHAIGTAISRDIIEYGIFKKKKIKETTIVLLT
                     RCGILVSLIVTIILSLELGGGIIARATAIFFGLMASCFLAPYTLLLFWKKITAKGAIS
                     GILGGFFVSLFCFLFLHGKEAGVFGLCKALTGSETLLPGVWAVVDPIVFALPASVLLT
                     FFVSLFTKVHNENTVEKAFKGIIQEKY"
     gene            complement(13892..14092)
                     /locus_tag="P5123_02885"
     CDS             complement(13892..14092)
                     /locus_tag="P5123_02885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012065833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRX15238.1"
                     /translation="MSKYGDLSVILAILFSLISLVLCIVYGALNWNKGGNISPEEAQK
                     EQSWDKEEAHISAELGQGGCDK"
     gene            complement(14254..15570)
                     /locus_tag="P5123_02890"
     CDS             complement(14254..15570)
                     /locus_tag="P5123_02890"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRX15239.1"
                     /translation="MKKLYTFLLLSIFAATDILASGLSTCDFNYINDLVGGRATGMGG
                     AYTAISDDPSGAFYNPAGLVYAIDNQISLSVNSYKNRKSVYKSVIGSNDYEQEVSSFY
                     PSFFGVTQSFGQVKIGFTFATLNNEILDQDSYFDDLTLPIDGTDAPTTYNMNYNITEN
                     TILAGPSVALFATDNLSFGLTLYIMKKAKQEIALQMITYEYNGAKRYSQDSTYITEDV
                     WAIKPVLGTQYMPNKYIALGLSISPGFVLSHTKDTQLFRKPYAGGNDMNQTNPDGDPL
                     ITFKTADNDIDDSKIPTLVRLGAAYYPSNKLIFTTDIIAHIGSQNLQNDVENTFNIAL
                     GTEYYLTASFPIRFGIFTNFANTPNIDKSKANQEAHTNLLGLSSSLSWQTRNSSITLS
                     GFYQASEFFGISPLGEGEAQVYAGSTTVQEMQMTLYSVSLTGSARY"
     gene            complement(15581..16402)
                     /locus_tag="P5123_02895"
     CDS             complement(15581..16402)
                     /locus_tag="P5123_02895"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: protein motif:HMM:NF013594.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M48 family metalloprotease"
                     /protein_id="HRX15240.1"
                     /translation="MRTKQKYLASIFFVTFAILSCTVGDNVRKRETEQHQQVTQELLY
                     GRLLSKEILSRYKVYNNPEASVYINKVGRGVALFAGRSELTYYFAILDSETINAFAAP
                     GGYIFITKGSIMAMENEAELAAVLAHEIGHVNNRHIMRQIPLPKGNNIADTIASILVA
                     QGTVVSAAMNETVKKASTLLFENGYRIQDEYEADQSAISYLIETGYDHRALIQFMSRI
                     ESIKKADAKLQVYHTHPPTLDRINMINSFLKEQNSASITGKLVTERFQKFKTSIH"
     gene            complement(16444..16938)
                     /locus_tag="P5123_02900"
     CDS             complement(16444..16938)
                     /locus_tag="P5123_02900"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRX15241.1"
                     /translation="MKIRLLSFLIFFSVMPLFAQTAYTTSLKTVIYSEPSLGSEKVAQ
                     LTQGTAITINKTHGKWVYISSASKNGWIQKAFTSTVKPKGKVSILGNANRTSRMKARE
                     RASSDVTAASARGFMQTENSKGRSRPNQEALKFDPADIDKMESVTLSEDELITFLRQG
                     GLLD"
     gene            complement(16950..19208)
                     /locus_tag="P5123_02905"
     CDS             complement(16950..19208)
                     /locus_tag="P5123_02905"
                     /inference="COORDINATES: protein motif:HMM:NF017076.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CHASE2 domain-containing protein"
                     /protein_id="HRX15242.1"
                     /translation="MNFIKIITRFNKKRKAKYKAFFVIALVMTILFAFLHIGFSSYFY
                     NKSYDLMVKNIGSSIDEPHEDIVLILIDQTSMIWGEELQLGRWPWPRNIYSDIITYIN
                     EKDPPKAIFFDIMLSARDRSNDTNDILLAETIALNNNVFHNVFFSTNNELTTGMKMPD
                     DVTQNHSIVIDNAPSDFFDNDNYNEVELPLPCLRAEVSCDTKQTDVHDKRPTAMGVSV
                     VTTVFPDDDGIYRKSKPVAKYGNHFYPSMALSAILAYQGLSNKVTWQNNNIVAGNYTI
                     PLDTNGNYLINYHTKNRIKSYSMSWLLDSAIDYYSDNNTDIKLPPGSFKDKIVIIGVS
                     APAGQDLKNTPISKTTPGPEIQANMISNILQNNHIINSSGTSSFLLSILLMFITVYIV
                     VFKGNPLVKILSNVIIYTVFAVVSAFIFYKTNYIVNSIFGLTTGVIATTGSYMFLSIT
                     EGAERRKYSKILGSMIDPIIVKEALNDLDALKRGGEKRITAFFSDVASFSTISEKLTS
                     AELAALLNEYLSAMTKILKTHRGTLDKYIGDAIVGIFGAPVGYSKTALLACKASLKMQ
                     QELSILREKWIADHAYCPEAREMQFRIGLNTGIAKVGFMGTSELASYTMMGDTVNLAA
                     RLESAGKDYGVHTLISQYTQKEVKNEMVTRLLDKVRVKGKEKPVAIYELIGEKGSISQ
                     KILDFIELYEQGVELYQNRQWLKAAKTFTKANKTVQNSDKSCSLLIARCSYYHKRTPP
                     TTWDGAFTRTHK"
BASE COUNT         5716 a         3487 c         4189 g         6108 t
ORIGIN      
        1 aacaccaggt tccatcggtg cttttaagtg gaaaccatgc ggaaattgcc aagtggcggt
       61 taaataaaag agtggaaaaa accaggcgtg tgcgcccgga tttgtataaa caatatgaaa
      121 ttatcaaaga gaaacagagg tgatatagat gaatgctgtt gaaaagctgg gattagagct
      181 ttctgaagtt aaaagagaag tagattttga agttggagat accgtacgtg ttcattacaa
      241 aattattgaa ggaactcgtg agcgtattca gatctatgaa ggagctgtaa tcgcgattaa
      301 caataaatgc gctggtaaaa cctttactgt tcgccgtgta tctttcgatg tcggtgttga
      361 gcgtgttttc ccgctgcttt cgccacgtat tgctaaaatt gaagttattc gtaaggccaa
      421 ggttcgaaga tccaaacttt attatcttcg tgagcgtact ggtaagtcgg ctaaacttaa
      481 agaaagaaga catatttcca agaaataggc tttttatgac ggctgagtct ccctcttttg
      541 aatatgaaca gatctattcc aaagggggac ctatagccgg ccttgatgag gcaggccgcg
      601 gagctctagc cggtccgctt tatgttggtt gtgttgtttt ccctccttct tattatctga
      661 atcctccttc agaaatactt gtgattaatg attctaaaaa aattcctaag aaacgacgtt
      721 tgcaagttta tcaaattgta atagaccatg cactttttta cgattttgaa ccctcttgcc
      781 cagatgaaat tgatacatat aatataaatg gtgctacgcg ccttgcggtt aatcgcttac
      841 ttgaccgtgt cccctttgaa atttgtactg ctattttgga cggaaatttc agatttgcat
      901 caaagcaccc acttgtttcg cttaaaaaag gtgattctct ttcattatca gtggcggcag
      961 cttcaattat agcgaaggtt cagagagatt catttatgag tgatctgaaa agtgaaaagt
     1021 atccttatgg tttcgataaa aacgtaggtt atggcaccag gtttcacctt gatgccatcg
     1081 gtaaatacgg gccatctgat cagcatcgaa gaagctatga acctgtcagg tcgctgtttt
     1141 gaagatcgaa aacgccataa aaattgaagg tgctctttct cgaaacaggg ctttgacatc
     1201 attatcgcct ggagacagtg tgcgagtggt gattctcgaa aagattgaca atgcgcgtgc
     1261 tgtagtgaat cttttaggta acaaggtgac agcgcagttt tcggaaggtg ttcctaatga
     1321 aaaaactgtt ttgctaacga ttaccggtaa tagcggaggc aaattggttt ttaagcgaca
     1381 aaccggctct tctgaagcaa tgacgtttaa atctgtttta ttaggcgaca agataccgga
     1441 tcagctgttt ttgcgttcta ttggagcggg ggtttcggat atttttactt ttaaccgtct
     1501 gctgctacaa caccgcttaa aaaggcgtat aacagaccac aaaaaaatat ttagccggct
     1561 actatcacag ggttttagcg aaaaagagct ttcgagtctt tccatttttg gattgaaaaa
     1621 gatgccatct ataataacac atttttcaga tttgtttgtt gaaaatagtg tatccgatag
     1681 cgagctagag gagctggaaa ccaaactagg gaatttaagc aatgaggact tgcaaatgct
     1741 atttgattct gtttttgatg aatcatcggc gttgtttctt ttaaatattg acagcacata
     1801 ccgctggttt aatatcttgt ttgaggaaga ttatatctgt atcagtttcg agttcgagaa
     1861 atctggttta attcaactgg ttgcaaaaga agatactacg gatataaata tttcactgtt
     1921 tttcgaatcc ggtgactttc gtaaaatgtt tatgtcggaa tttgaaaatc ttgacaagat
     1981 tattcgtaac agcaataaaa agattaggct tgtaacttgg ctcttgtcgg attcggtgac
     2041 aatgcttaat aaagagataa accgtgctgt gcaaaatccg gttttggata taagagcttg
     2101 ataaataaaa gtaaaaaatc tattgctctg aagtatgacg caaaaattga tgcagctcca
     2161 tatgttgtag ttaaggcttg caacgagctg tctgatataa tgttaaaaat tgcccgtgaa
     2221 aagggaatac cagagtacaa agacgacgat ttggctgaag cgctttatgt gttgccagag
     2281 gggcagcaaa tttcacctga cttatataag gcggtggcag aagttatagc atattgttac
     2341 agtattaaag gaatggatta ggcctgaatg aaccgtattc ttgtagaaga gcttaaaccc
     2401 ggcatgaggt ttaccaagga agtcatgatt gacagcagca atgtccttgc cgctgccaat
     2461 gtgcctatcc aagagtcgga tattaagcgt cttatgaaat ggggaataag cgaggttacc
     2521 accgaaggtc agcttttaac gggggcagct ggcggtggta gtgttgccat atctgaacag
     2581 tttaacaagg ttctggatga ttataatgtc ctgatctctc ttaaggatca gctaattgaa
     2641 gttcatactc gtactgtaaa agaagtgtct cgtatacata cggcgatacg caatgatcgc
     2701 atgttcaata tccaggagtt aactaaatgt gttgaggata tatataacct gattaggcaa
     2761 aatagaaatg tttttctttt tgttcaacga caggaaaatg aaaaagatac aatggcagta
     2821 catagtgtta actcggccat ttatgcaatg gtaataggat tgactctcga gtattcacgt
     2881 tcaaaggtaa aagatcttgg cattggcgtt ctgttgatta atgcgggtat ggttcagatt
     2941 cctttatata tcgtgaataa agagggtgac ctgaacgagc gtgaattgca acagattaaa
     3001 agccatccgg ttttagggta tcaggtcttg aaaaatttag gaaatatatc agaggatata
     3061 gcacagatat ccttacagca tcatgagcaa tttaacggag gggggtatcc caaacggctt
     3121 aaaggtatcg aaatatccga agccgcgcgt attgcatctg ttgctgataa ttatgaagct
     3181 atggttgaaa aaaagagtta tcgccctagg catttttact atacagctat gaaacaatta
     3241 gttgctaccg gcgcccgtaa atttgatccg gtaatactta gaatgtttat atctattcta
     3301 tctgtatatc cgataggttc tcttgtggaa cttaacaata aaaaaattgg tgttgtgata
     3361 ggcactatca aggataatcc tttgcgacca atagttaaaa tggttaggga tgagaaaggt
     3421 gaacgggtaa agggtgtcga aattatcaat cttgttgagg agtttgatct ttttattaca
     3481 cagacgattg acgagtacaa agctggcatt aatctttccg agctatttta atggatgatc
     3541 gggcagacaa aggtgggcgt ggtgaacaac ttgctgttga ttatcttgaa aatcagggat
     3601 ttaccatatt aacacaaaac tatcgctatg gacgttatgc cgaaatagat attattgctc
     3661 aaaaggatga tttgctgctt tttgtcgaag ttcgatcaca ggatactttt cagatgggtt
     3721 cagccctcca ttccgttaga cagagtaaaa tagataaact tcgtaaatgt gcccgctctt
     3781 atctcgcttt tgtttatccc caagataggg aagtagattg ccgttttgat ttgataacca
     3841 tagacaaggg tgaattaacc tggcatcagg atatatcaag ataaattgaa ttaattctta
     3901 atcactctcc ataatcaata aaatgcataa taatcacctc taaaaattgg tattttcatt
     3961 aaaaacagat aaaaattgag attttatcat gctttatgac taaaaagagg cattagaagt
     4021 tcatttttag tggttttctt gtgtttgagt tgtgtttggt ttattcgttt ttttttatca
     4081 attataaaga aatactaatc aaaaacggca ttttgcccga actttttatt aggtatgatc
     4141 tccctgttca aggaggaaca ggatgccgaa aaaggagttt cgtcatgaaa aatctcatgc
     4201 tcaaggaggg acgtgagatt gagtcaggtt tgggtgtaga gcgcagcagt acaagagttg
     4261 ataaatacaa acaactaatt tctgatgaga gctacatcga ttttgctatt acgcgtatag
     4321 caacggatct gtctcattac ctcactaaat gaatgccctg caatcatggc cggctatatt
     4381 agccggcaac tcctcacata aaagccggta aaacggtttt tatgctttta acggtcaaaa
     4441 aaattgtgtt gaaatgaacc cagtacagga taaagtagtc cgatattcca gttaagccga
     4501 atttgcagtt tatggctgaa aacaacaaaa attatcaata atattgcgtg cttgccgtgt
     4561 cggaggatgg atgcctgtaa ctcgtagaaa tttcttgaag atcgcgattg gccttttggg
     4621 tatggctgcc gcagggggta taaccctttt tgaatataca agacgtatcc ctctctttaa
     4681 aaaaagaagc aatagcataa ggtctttttg tcctttttgc tctcttaatt gtgcggtaaa
     4741 aatatattat actgacacaa aaattacatc tgtacagccc gaaaaaaaat catttgaaag
     4801 taacggacga atctgcccta aagctcatat gcttcgtgaa atgtttaacc gacccgacat
     4861 aataagcttc cctcttgttc gagaggccgg ctctgatgtt tttctcaaaa cagattatag
     4921 cgatgtatta aatcgttgtg ccgaaacgat taatagaaca cgggaagaaa cttttataac
     4981 agaaagctca ggttttaaaa taaattcgtt ttctggggcg gctgtaatcg cgggtgataa
     5041 aattggttgt gaagatgctt atgtaattaa taaatttagc cgtgttatcg gaatttcacg
     5101 tgttggcagt ggcgctctta ttcgccataa tagctctgta atggctttgt gctcgacttt
     5161 tggtatagcc gcacagcaca atcctgtgta tgatattgta aattcggatg tagtttttat
     5221 aattggtgcc aatcctgcac gtaccaatac aatgcagctc tgctcgattc aggaagctcg
     5281 aaaacgtgga gcaacaatta ttgtagctga tccaattgcc ggagaaactg cagctctggc
     5341 tgacatatat cttccgttgc ttcccggtac agacactatt ttgctgtgcg ggcttatacg
     5401 ttatctgtta cataataacc gctatgacgc ggagtatcta agggataata ccgacgcgtc
     5461 atttcttgtt aatgagtctt ttttagtaaa tgcagatgga acctttagcg gatataatgc
     5521 cgaaagtgca tacgatacat tgacctggtc atatattatt gatgaacgtg gaaatcctcg
     5581 aaccgatagc agtatgaaaa aaattaattg cgtgtttcaa attctaaaga aaagttattc
     5641 tgcatatact cccgataatg ttttaaatat gactgggctt ggtgcggatc tttttttaaa
     5701 ggctgccgaa acactagcct ctgtttcgct tcctgctaaa agttgctctg ttatctttgg
     5761 gggaggtttt actcagtcat caggtggaca gggtgttcga gccgtttcga ttttacagct
     5821 tttatgcggg aataccggta tagcaggtgg tggaatatac ggcatcaatg atcgatctaa
     5881 tcaacaagga gtaagtgatc agataggtaa ctggaactgc cttcctgggt atcttcccat
     5941 tcctgatagt agtaaaatgg aaaatgatta cgaatcttat attaaaaata acagcaaaaa
     6001 gagtaatgat tttagtgtaa gtaattattg gaataatttt caaggatatt ttgaatcaca
     6061 gatgaggtgt tggtttaaat ctataagtcc tgaaaaggct ttttccattc tgccacgcgg
     6121 atcttcaaat tacagttttt ctaaacttct tgatgatgtg ggaaaaggat caattaaagg
     6181 attgattgtt tttgatgagg atatctttgc ggctccagta tctgaaaacc gtataattaa
     6241 tgccttgaat aaattatcat ggataataat gtttgtatcc aatcaaaatc ggctaagtcg
     6301 cttaatcggc aagctttcct tctctaaaaa tgcttctgtt attctcgttc cccttccgtc
     6361 ttatattaat tctgaaatgc acttcgtatc ttcagaccgt cgattcaatg cttatgacgg
     6421 aagtgcagtt tttaaacggg gagccgctat gtatccgcaa attgcattcg cgttacaagg
     6481 tattgcaaaa aaaatagcgg agattcacaa agccaacggg ggagtcttca gtgatccgat
     6541 tattgacagt gaatggagat attctacaat aaaatctatc gcgaaagagc ttggtggagt
     6601 aaacagagca aatggtagtt ctgataataa tatagagcaa gttttttctg aaagttatgt
     6661 ctgcggcaac tggctctact ccgatattat tggtgataaa aggcttttaa aacgggataa
     6721 gtattcacaa aatggattgc ttcgtgatac tgataggtgt tggccgagcg gtgaacaagt
     6781 tctttttaat cgagctggag taaattttaa ggacggaagc cctcgacatg gtaaatgtat
     6841 tgttttcgaa gatgtaaaaa aaattaaaaa tgaagaactt gcacgcaaac gaccttttcg
     6901 ttttacaagg gatggggtag cctatctttt tgcacgagaa atgatttccg gaccacttcc
     6961 aatatctggc cttgaaataa aagatgataa taatgatttt ttacttttta catcgagttg
     7021 gagtgagtcg tcgttcgcgg aaatgttcaa ctattataac gagtattatc cggaagattg
     7081 tattctgatg aatcgagaga gtgctgtgac agctggtttt gaaaacggaa aaaatgttgt
     7141 tgttcaatct tcggtagcta aaataactgt tccactttat ataaccgaac gaattgccaa
     7201 taaacgtgca gttaacggta ttggtgttgt ttttaaagag cttttggaca atattgatag
     7261 tgatattatt acacctgtaa cggtcaggat ttacaaggag atctcgaatg gcaggtgaaa
     7321 agtgtatctt gatagattcg tcaaaatgtt gcggctgtgg aagttgttat tttagttgca
     7381 aatcttggaa ttcaataccg gcaagtggag gttccagaat tactgacggt gagttctctg
     7441 ttattcggga tatgcggcca caaaattggc tgattgtaaa gagaagtgcg gatgacggac
     7501 gtttgagggc atggccggat ttatgccgtc actgtagcga tgctctatgc atcaagcatt
     7561 gcccagtgga ggctatttcg aatcaggatg gctgggtaac cataaatagg gatatttgta
     7621 tcggttgcgg tgcttgtgag gttgtttgcc catatggagc tatctctgtt tacaggggag
     7681 attcaaagga cgttttgaaa aagaatcgag cctataaatg tgacggatgt tcagagaatt
     7741 attcagaaaa acccgcgtgc gttgtctctt gtccaacggg agcgttggaa tacggtaacc
     7801 gttcacggct gatacgtaaa gcgatgcgtc gcgtaaagaa gttaaaaaaa gagtatcccg
     7861 aaacaaagct atatggaata aatgaataca agggacagaa tgttctgttt ataaatatga
     7921 aaaccgttga gcatgagaat aacgatgaac agctggggta taatcttttg caggtaaaaa
     7981 ccctatataa aactgtttca atgtttactc gttcattttc agtaatgtct ggccatatat
     8041 acggattttt acattttata gttacaggca aaaaaaatgt gtagtataag tatacgattt
     8101 taacaattac cgtatattat ggttaaattt ttcatagaat gtgtaaaagt attaaaaaat
     8161 cttggctaga tggtaattac ttgaaaaagt aattatcaat agttgcttgc agactgtaat
     8221 tttttgttgc gcaataagta atatattaaa aatatagcgt taattaaggt ggttagagag
     8281 gcttatatga aaaaaataga ggtattgatt tttgttgttg ttttcttatc tgttagcagt
     8341 tgctccacac tgcaaagttt ttttaaaagt gaaatgaccg ttgcacaggc aattatggcc
     8401 aaagatcgaa tggataaaat aacgaatccc gcacagaaag atattatgta caaggatttg
     8461 caaaataaga ttattgttgt gccccgcgcg aaggttttgg acataataga gtccatcaat
     8521 gttgattatt cttatgctgt tatagctcga gtagaaaccg aaaaaggacc ggttgatctt
     8581 tatctttaca gtaaagatca aaagctgatt gcatcattaa gcaaggaaag ttcagagatt
     8641 tctgctaagg gagagttttc caaatttttt caattgctcg atgattctta tgccaaaatt
     8701 gagcttgtaa aggcaaaaat caataaattg taatcacgtt ttcgattttg gaataatgaa
     8761 tatggtttaa ttatcaaata tagcagggct ttccattgag agaaagccct gttgttgttt
     8821 tagcgctcgt ctatgacgcg aatagcctta ccttgctgaa cgggaagatg ccccggttca
     8881 tgaaattcta tctgcggaga gattgttata gatgctttta gctcgtccct gatctggtct
     8941 cgtaaagcat aaagttttct tgtatcatct gatattatat cagagttgac ttcggtgtca
     9001 acaatcagtt cgtccagcgt tcccttcttt ctgacaacta ttcggtagtt gttaccgata
     9061 tttggcattt tcataattac ttcttctatt tgcgaaggaa aaacatttac accgttaata
     9121 ataagcatgt cgtcggtacg acccagaata cgttctatac gtctgtgggt tcttccgcat
     9181 ggacattcgc cggggattat tttagttagg tctctggtgc ggtatcttat tataggtgtt
     9241 gattcacgaa taagatttgt aagaacaagt tctcccgttg taccttccgg tacaggggta
     9301 agtttctctt tatctaccac ttccataatg tatgaatctt cccaaagatg catgcctttc
     9361 ttatattcgc attcaaaggc tacactgggg ccgttcattt cagacaggcc gtatgagtta
     9421 tacgcatcaa tttgcaggta atcttcaatt ttttgacgaa tctcttccga gtgcggttca
     9481 gcacctatca tagctttctt gatgacaaag tcttttcggg aataacccat tgactccatc
     9541 tctgaaaaca gatgtaacag atagcttgga gtagcgtgga caacagttgt ttgaaaatct
     9601 ttcattaaca taaactgtcg tttggtgtta ccgcttgacg cgggaattac aaacatacca
     9661 agcttttctg caccataatg cagaccgatg cctcctgtaa aaagaccgta ggtcatcatg
     9721 ttctggaata catctttttt tgtgcatcct gtaaccagaa gagatcttac agtaagattt
     9781 gtccagcggt caagatcggt ctgggtttga aatatcactg tgggttttcc ggtcgtaccg
     9841 cttgaagcat gcattcggac tacttcttct ttatcaacag cgagaagtcc atacggatag
     9901 gtatcgcgta aatcttcttt tgttgtaaat ggaattttgg taatatcgtt gatatgtgta
     9961 atatcagatt gatcttttat tcctacgctt ccaagtcttt gtttatagaa ttcagttttt
    10021 aatgcctgtt taactatttt tttaaagcga ataagctgca gtgcttcaag agcatcccgg
    10081 tcaagatgtt gaatttcgta atcgttaaag caagaagtca tattgatccg cccagttcaa
    10141 ttgcacgaat gtttttgttt ataatatctt cgcttttgtt tttaaagagt atcgctacag
    10201 ctttttttag tgattcggtt ttgacgggaa gatatcggga tgctgctcct acaattgcca
    10261 tgtttgaagc ccttgggttt tgagactctt ttactatatg tgcggtatca acaagctttg
    10321 aatttttcat tttttttatt tcagtgtgaa tttgatctag ttcgggataa tttggtatat
    10381 tgataaaggg ctcttgagcc gagattacta ttccttctgg ttttagaaac gaaatatagc
    10441 gtaaactctc aagtggttcc atgctgagta ccatgtccgc agatccttcg gcgacaaggt
    10501 cgctatggat ttcagtgtcc gatatgcgta agtgagaaag aacagctcca cccctttgag
    10561 ccataccatg aacttcggat tgccgtacat taaggttttc gattccggcg gcggtagcta
    10621 ttgctgctgc tacagataaa acaccttgac cgccgactcc gcagagtata atatcatatt
    10681 tcattagtta tctccttttt cggcttttct ctttttacgg aaatattcga tacattcacg
    10741 aaccggaatt atcagcgaga ccccgttaaa atctagctct ttacgtaaaa gagccgcatt
    10801 tgagtcatga tcatttttat gggcgtttac aacatgaacg tggtcgggat gaactccaac
    10861 acctaacgcg atttgcttta ttctttcaga aggtaaaatt gtctcttgcc ctcccgtcat
    10921 acccactgtt gaattgtcca gaacgagtat ggtaaccggg gcgttgtcag ctacacagtc
    10981 tactagtcca gtaagccccg agtgaaaaaa agtgctgtca ccaagaactg ccataacgtt
    11041 tttcattccg gattcggcgg ctcccttggc cattgttatt gacgcaccca tacaaacaat
    11101 agtctcggga acttgtagtg ggggtagcgc tccaagggca tagcagccaa tatcagacat
    11161 taccgcactc tctttaagtt ctgatacgac ctctttgata aaggtatagg tgtcatcatg
    11221 aggacatccc ttgcaaagtt ggggagggcg tccgggaaga ggcatgctac ttgaaattgc
    11281 actagctttg ggtgaaagac caagcacgga tcgtattgtg tcgggtgtaa gttccccttc
    11341 tcgtggaatc gctccagaaa gtttgccctt tacatcgata gttaaaggca gtatgcccct
    11401 taattgtttt tcgatcaaag gctcgccttc ttcgattaca atcagtgttt cgctcaaaga
    11461 agcaatttta ttgattttat cgtaaggtaa aggataataa ccaatatgaa gatggttaaa
    11521 ttcttgggaa cattgatcaa gattttctaa aaaataattt ttaccaagac ctgatgttat
    11581 tacaccaatt ttgctattct tgagaactag tgggttccag tcagttgttt cagaaatgct
    11641 tttcatctct ttctcttttt ctaacaaaga gtgccattgc tttcgtgcaa gggcaggcaa
    11701 aagcatccat tgctttacgt cgggagcctt gaccccagtg ttctcttttt ttcttgaaga
    11761 agtttttatt gctgcgcgag aatgagagag gcgtgttaca agacgcacca taacaggaac
    11821 atgaagtttt tctgaaaggt caaaagcttc actcattaca tcgtaggcct gctgctgatt
    11881 taccggttca aaacagggaa tatgcgcaaa ctcggcgtaa tagcggctgt cctgttcatt
    11941 ttgggaagag tgcattccag ggtcatcggc aattgctata accaggccac cccgtatatc
    12001 aagaagagcc gaattcataa aggcatcggc tgcaacattt aagcctacat gtttcattgt
    12061 tacaagagtt ctttttccgt aaaaagatgc tccaagagcc tcttcgtatg ctgttttctc
    12121 gtttgcgctc caacgggcaa gtggttttcc ctcttttttc tcgatctgtt gaaggtattc
    12181 cattatttcg gttgaaggag taccgggata accgtatgcc gcggagagtc ccgcgtctac
    12241 agcaccaagg gctatggctt cgtcgccaag aagtgcgtaa gtttccatta atatttctcc
    12301 tgaattattc ctttgaaggc cttttcgact gtgttttcgt tatgaacttt ggtaaaaagg
    12361 cttacaaaaa atgttagcaa tacggaagcc ggcagggcga atacaatggg atcgacaaca
    12421 gcccacacgc cgggcagaag ggtttctgat ccagttagtg ctttgcataa accaaaaact
    12481 ccggcttctt tgccatgcaa aaatagaaag cagaataggg aaacaaaaaa accgcctaat
    12541 ataccactta ttgctccctt tgcggtaatt tttttccaaa agagaagtag ggtgtaggga
    12601 gccagaaagc atgatgccat taatccaaag aaaatggctg tagcacgggc aataataccc
    12661 cctccaagtt caagagaaag aataatggta acaataagtg atacaagaat tccacatcgc
    12721 gttaacaaca caattgtggt ctctttaatt ttcttttttt tgaatatgcc gtattcaatg
    12781 atatctcggc taatagctgt gccgattgcg tgatattgcg agcttaaagt cgacatagcg
    12841 gctgaaagaa ttaccatcat aaaaaggtaa acaaaccata tcggcatggt ttctccgata
    12901 aaaacaggaa taactcggtc aaggttttta ccagcagctt caaaggcgat atgaccatat
    12961 ttttcgaaga aaaacacatt cgatagagga ccaacaagat aagttgttcc gacggtcgca
    13021 aaaataaaga gggctccaat cgctacggct cgattaagct ctttgttaga tttaactgtc
    13081 ataaacctga cggcaagttg tggttgtgaa agtactccaa gaccgactcc aagaactagg
    13141 gacgaataga taacccacca tagggatgac ccggtttgag ggcttgatgt ccagctaaaa
    13201 actccggagt ttttgatgtt ttcagggata gcatttgcaa gttgagtcag agagctgtgt
    13261 gcttcgaaaa caccgcccag ttttgtgtat gtatatatta gcagggtaac cattcctata
    13321 aaaatcaaag atccctgtat ggcgtctgag tacattacac ttcttaatcc acccataata
    13381 acatatccac ccacaagaac agaaaaaacg agaaccgaca gctcatatgg aacaccaaga
    13441 aagctttcaa tgattcgcgc agcaccgatt aatacggccg cagcataaat tggcataaaa
    13501 ataaaaatca taagaccagc aaaaagttgt ataaatcgtg aatcaaaacg tcgacccaaa
    13561 agttccggaa aggtgtgagc atcaaggtta aggcccataa gtcgggttct tttcccataa
    13621 aatacgaatg ctatgaaaat tccgacaaat atattcgcga aacagagcca cataaggctc
    13681 attccgtaaa gtgacgcgac tccaccaaaa ccgacaatgg cggaagtaga tataaaagtt
    13741 gcaccgtaag acattgccat aataaagggg tgagtattgc gtccggcaag catataatcg
    13801 gaagagcttt tggaagcagt gtatcctcga tatcctagat aagcgataac tgttagatat
    13861 attagaataa tgataattaa tacggtatag ttcatttgtc gcatcctcct tgcccaagct
    13921 cggcactaat gtgagcttct tccttgtccc aagattgctc tttttgagcc tcttccgggc
    13981 taatattacc gcccttgttc cagttaagag caccgtaaac tatacataaa acaagagaaa
    14041 ttagtgaaaa gagaatggcc aatattacgc ttaaatctcc atatttggac atgacagcct
    14101 ccaggaatga ttttttacat tcttaactta tgcaattttt tcgtacagaa aaaaaccaga
    14161 cggacggttt gaattgggga ttttttggag atggtaaaag taagttttaa atggaaatag
    14221 gataagtaag gccgggtgtt ccggccttat aaattaatag cgagcagaac cggttaggga
    14281 tactgagtag agtgtcattt gcatctcttg aacagttgtg ctaccagcgt aaacctgggc
    14341 ttctccttct cctaaaggtg aaattccgaa aaattcagaa gcctggtaaa aacccgaaag
    14401 tgttatcgat gagttacgag tttgccagct taaacttgaa gatagtccta ataggttggt
    14461 atgagcttcc tggttagctt tgctcttatc gatattcggg gtgttagcga aattggtgaa
    14521 aattccaaac cgaattggaa aagatgctgt tagataatac tcggttccta aagcaatatt
    14581 gaaagtgttt tctacatcgt tttgaagatt ttgagatcca atatgagcga tgatgtcggt
    14641 ggtaaagatc agtttgtttg atggataata ggcggctcct aacctgacga gtgtaggaat
    14701 cttagagtca tcaatatcat tgtctgctgt tttaaaagtt atcaaaggat caccgtcggg
    14761 attagtctga ttcatgtcat tgccgcccgc ataaggtttt ctgaaaagct gagtatcttt
    14821 tgtatgcgat agtacaaatc ccggagaaat actaagtccc aatgcgatat acttgtttgg
    14881 catatactgt gtgccgagta ccggttttat cgcccagaca tcttcggtaa tataggtgct
    14941 atcttgtgag tatcttttag ctccattata ctcgtaagtt atcatttgta gggcaatttc
    15001 ctgttttgct tttttcataa tatacagagt caatccaaag gagaggttgt ccgttgcaaa
    15061 taatgcaaca gaaggcccgg cgagtatggt gttttctgta atattgtaat tcatgttgta
    15121 tgttgttggt gcatcggttc cgtctattgg cagtgtcaga tcatcaaaat atgaatcctg
    15181 atcaagaatt tcgttgttta gtgtagcaaa ggtgaaacca attttgactt gcccaaaact
    15241 ttgagtaact ccgaaaaaac tgggataaaa tgaagagacc tcctgttcat aatcattact
    15301 gccgataacg cttttatata cagacttacg gtttttatag ctgttaaccg aaagtgatat
    15361 ctgattgtca atggcgtaaa caagaccggc aggattataa aaagctcctg atggatcatc
    15421 cgatattgct gtgtaagctc ctcccattcc ggtagcacga cctccaacca aatcatttat
    15481 ataattaaaa tcacaagttg aaaggccact ggccagaata tccgtggctg caaagataga
    15541 taaaagtaaa aatgtgtata gctttttcat ttaatcctcg ttaatgaatt gatgttttaa
    15601 atttttgaaa tcgctcggtg acaagttttc cagttatgga ggcactgttc tgttccttga
    15661 gaaatgagtt aatcatgttt atcctgtcaa gtgttggagg atgtgtgtga tagacttgaa
    15721 gctttgcgtc ggcttttttt atagattcga ttctggacat gaattgaatc agcgcacggt
    15781 gatcataacc ggtttcaatc agatagctta ttgcactttg gtctgcttca tattcgtctt
    15841 gtatacggta tccattttca aatagaagag ttgatgcttt ttttactgtt tcgttcattg
    15901 cagcagagac aacagttccc tgagcgacca gaatggaggc gattgtatcg gcgatattgt
    15961 ttcctttcgg gagtgggatt tgacgcatga tgtgacggtt attaacatgg ccgatctcat
    16021 gagcaagcac cgcagctagt tcggcttcgt tttccatggc cattattgag cccttggtaa
    16081 taaagatgta gccacctgga gctgcgaacg cattgattgt ttcactgtca agaatggcaa
    16141 aatagtaggt tagttcgctg cgacctgcga aaagagctac gcctcgacct accttgttga
    16201 tgtaaactga tgcttcagga ttgttgtaaa ctttgtatcg ggacagtatc tcttttgaaa
    16261 gcagtctgcc atagagaagt tcctgggtta cctgttgatg ctgttcggtt tcacgttttc
    16321 gtacattgtc acccacggta catgatagaa tggcaaatgt tacaaaaaaa atcgaagcca
    16381 aatatttttg ttttgttctc attttagaac cttaatctgg gatttgcaag atttcataaa
    16441 tcattaatcc agaagaccgc cttgccgaag aaaagtaatt aactcatctt cacttaaggt
    16501 gacactttcc attttatcaa tatctgcggg atcaaattta agcgcttcct gattaggacg
    16561 actgcgacct ttggagtttt ctgtttgcat aaaaccccta gcggatgcag ctgtcacatc
    16621 agatgaggct ctttctcgtg ctttcattcg tgatgtgcgg ttggcatttc ctaaaataga
    16681 tacttttccc tttggtttaa cagttgatgt aaaggctttt tgaatccagc cgttttttga
    16741 agctgaactg atatagaccc acttgccgtg agttttgtta atggtaatgg cagttccttg
    16801 tgttagttga gcaacttttt cggatcctag cgacggttcg gagtatatta cagtctttag
    16861 agaggtggtg taggctgtct gggcaaacaa cggcataacg ctaaaaaaaa ttaaaaatga
    16921 aagtagtcgg attttcatga taacgcctcc tatttatgtg ttcttgtgaa tgcgccatcc
    16981 caagtggttg gcggtgtgcg tttatgataa tatgagcagc gggctattaa gagtgaacaa
    17041 gacttgtcgc tgttttgaac agttttgttt gcttttgtaa aagttttagc ggctttaagc
    17101 cactgacggt tttgatacaa ttcaacaccc tgctcatata gttctataaa atccagtatt
    17161 ttttgagata tgcttccttt ttccccaatt aattcgtata tagctaccgg tttctctttc
    17221 cctttgacac gaaccttgtc aagtagccgt gtgaccattt cgtttttgac ctctttttgt
    17281 gtgtattgac tgataagagt gtgtacgccg taatcttttc cggcgctttc aagacgggca
    17341 gcaagattaa ctgtgtcgcc catcatcgtg taagaagcta actctgaagt tcccataaac
    17401 cctactttag cgattccggt attaagacca atgcgaaact gcatttctcg ggcttcaggg
    17461 cagtaggcat gatcggctat ccatttttca cgaagaatgg aaagttcttg ttgcattttc
    17521 aatgaagcct tacaggcaag aagtgcggtt ttagagtatc caactggagc accgaatatt
    17581 ccgacaatcg cgtcgccgat atacttgtca agagtgcctc tgtgtgtttt aagaattttg
    17641 gtcattgcag atagatattc gttaagtaac gctgcaagtt cggcactggt aagtttttca
    17701 ctgattgttg aaaatgatgc aacatcagaa aaaaaagcgg taattctctt ttcaccgccc
    17761 ctcttgagtg catcaagatc atttaaggct tcttttacta tgattggatc tatcatgctt
    17821 ccaagaattt tgctatattt tcttctttcg gctccctctg ttatgcttaa aaacatataa
    17881 gaaccggtgg tagcaattac accagtggtt aagccaaaaa tcgaattaac gatataattg
    17941 gttttataaa aaataaaggc cgaaactacg gcaaacacag tatatattat aacattggac
    18001 aaaattttta ccaaggggtt gcccttgaaa acaacaatat aaacggtgat aaacatcagt
    18061 aatattgaaa gtagaaaaga gcttgttcca gaactgttga tgatatggtt gttttgaaga
    18121 atgtttgata tcatgtttgc ttgtatttcg ggaccggggg tggtttttga tataggcgtg
    18181 ttttttagat cctgtccggc tggtgccgaa acgccgataa ttacgatttt atccttgaat
    18241 gaacccggtg gtaatttgat atcggtatta ttatcgctgt aatagtctat tgccgaatca
    18301 agaagccagc tcattgaata gctctttatt ctgtttttgg tgtgatagtt tataagataa
    18361 ttcccgtttg tatccagagg tatggtgtag ttaccggcaa ctatattgtt gttttgccag
    18421 gttactttgt ttgataatcc ctgatatgct agaatggcgg aaagtgccat ggagggataa
    18481 aagtggttgc cgtattttgc tacgggtttt gattttcggt atataccgtc atcatcagga
    18541 aatactgttg ttactacaga tacacccatt gctgtggggc gtttgtcatg aacatctgtt
    18601 tgttttgtat cgcaacttac ttcggctcta aggcatggaa gtggaagttc tacctcgttg
    18661 tagttatcat tgtcaaaaaa atctgatgga gcattatcta tcacgatgga gtggttctgt
    18721 gttacatcgt caggcatctt catgccggtt gttaactcat tgtttgtact gaaaaaaaca
    18781 ttatgaaaaa cattattgtt tagtgcgata gtttcggcaa gcagaatgtc gttggtgtcg
    18841 tttgagcgat ctcgagccga taacattata tcaaaaaata tcgctttggg aggatctttc
    18901 tcgttgatat aagttatgat gtcactgtaa atattccttg gccatggcca tctgccaagc
    18961 tgaagttctt cgccccatat catggatgtc tgatcaataa ggatcaggac aatatcttcg
    19021 tggggctcgt ctatgctcga accaatattt ttaaccataa ggtcgtaact tttgttgtaa
    19081 aagtaggagg agaaacctat atgaagaaag gcaaagagaa tggtcattac caaagctatg
    19141 acaaaaaatg ccttgtattt agcttttctt tttttattga atctggtaat tattttaata
    19201 aaattcatat gaatccttga ttcaaaacag attttaccta aaagatagtc atataaaatg
    19261 atttgtaaat aactttttat gaatttttat agactaacca aataaatcca cttcgctttt
    19321 tggaaaaaaa catttgatta ttctactatg tgaacataat ttttgtggtt ctattgaata
    19381 ttgctttccg gataaaagct attaattaaa gcagcaatca agttaaagct tgacttataa
    19441 tggagtggtt aaaacgatat atagagattt aaagttgaaa aaacccggct gtacggttta
//
LOCUS       DAOTGZ010000023        19248 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_3064
            NODE_3419_length_19248_cov_4.538790, whole genome shotgun sequence.
ACCESSION   DAOTGZ010000023 DAOTGZ010000000
VERSION     DAOTGZ010000023.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868088
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 19248)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 19248)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:22:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,785
            CDSs (total)                      :: 2,751
            Genes (coding)                    :: 2,732
            CDSs (with protein)               :: 2,732
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 12 of 19
            Pseudo Genes (internal stop)      :: 5 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19248
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3419_length_19248_cov_4.538790"
                     /isolate="AMR_MDS_3064"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..327
                     /locus_tag="P5123_02910"
     CDS             <1..327
                     /locus_tag="P5123_02910"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="triose-phosphate isomerase"
                     /protein_id="HRX15243.1"
                     /translation="TAAESRAVAMLKPNIVVAEPTDLIGTGMTSDDAYIETSIRAVKE
                     IDPNIFVLAAAGISSGADVYRVIRAGAEATGSSSGITLAEDRAAMIDEMIGAARRAWD
                     ERATAR"
     gene            complement(324..944)
                     /locus_tag="P5123_02915"
     CDS             complement(324..944)
                     /locus_tag="P5123_02915"
                     /inference="COORDINATES: protein motif:HMM:NF012497.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRX15244.1"
                     /translation="MKRFVNRSSVEGTVRAPSSKSMMQRAVAIASLGKSSCRLNGLSG
                     CADLFASIRAAEALGTKVELDGSSALITPRFRKASDTVDCGEAGLTVRMFAPIAALQN
                     HKFVFTGQGSLLKRPIDMVEDALIKMGVSCSTQNGFLPMCIEGPVSNNEITVDGSVSS
                     QLITGLLIAYAHCDKSSRIIVNNLTSKPYIDMTIEILSLSYHAAFA"
     gene            complement(913..2205)
                     /locus_tag="P5123_02920"
     CDS             complement(913..2205)
                     /locus_tag="P5123_02920"
                     /inference="COORDINATES: protein motif:HMM:NF013375.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate kinase"
                     /protein_id="HRX15245.1"
                     /translation="MIYAVCGKPVLHSKSPLIFNNYFKSCGSSDVYTRIAAESGHEII
                     SFLRSSGIAGCNVTAPFKDQVIPFLDRVDDEALRLQSVNTIKNRNGVLTGFSTDSYGV
                     YYTLLNCGKKYKDKRAVLLGAGGAARAAAVALDRLGLRVTVVNRTFEKAVSAANLCGG
                     TAVTPDNAEEAIRKADVVINTLLPEVDIIKSEWFKEDVLALDAIYKESVFQQKAMKAG
                     VRYVRGEEWLYYQADRAAEHFFGKRFGDIKPPESFMEPVKIKTGTITLTGFMGSGKSS
                     IAKNLAFCTEIPCYDMDEIIENSEGVSIADIFKTQGEEWFRQKECELLEKLCENNEKK
                     IISCGGGVVTRKANRDILRKKCLNIWIYADMDKSLERVANTNRPLASDGAEQFKALFE
                     SRKELYAGCADMTIINSDKDVNQSVRRINEEICEQIIC"
     gene            complement(2206..2853)
                     /locus_tag="P5123_02925"
     CDS             complement(2206..2853)
                     /locus_tag="P5123_02925"
                     /inference="COORDINATES: protein motif:HMM:NF013640.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I 3-dehydroquinate dehydratase"
                     /protein_id="HRX15246.1"
                     /translation="MSKLCVAVSEDSIEDIVRECSSADISEIRLDYASFSEKEINEIF
                     SSAKRKLVVTYRPGKVEEEFRQNMLITAVKAGAYYVDLEIENSREFNAPIIDAARLVG
                     CKVIISYHNYQLTPDNRELNEIIDKSISYGADIVKIACLARTQKDAARLLALYQREEE
                     MIILGMGDYGKITRVASLYLGAPFSFVAPDSGRTTAPGQLTFSQMQSIKDLIGDL"
     gene            complement(2825..3871)
                     /gene="aroB"
                     /locus_tag="P5123_02930"
     CDS             complement(2825..3871)
                     /gene="aroB"
                     /locus_tag="P5123_02930"
                     /EC_number="4.2.3.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012610567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003856 - 3-dehydroquinate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009423 - chorismate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-dehydroquinate synthase"
                     /protein_id="HRX15247.1"
                     /translation="MSDIFRIDYKTSGNSSKVLVGESLSSLSNYIPLKKSVIITDSNV
                     YMYYKDQFPDCPVLVMECGEEHKTIDTVAQLHNELIELGCDRSSYIVGIGGGIVTDTA
                     GFVASTFLRGVGFGFVSTTLLSQVDASVGGKNGVNHHGYKNMVGVFNQPDFVICDIEM
                     LDTLPDKEYINGLGEVVKHALIADNNMYDLINENIDLINERDKKILRQLIIRSIEIKT
                     EVVTEDEREQGRRRILNFGHTLAHALEKCIKIDHGLAVAQGMQFAAQISCIRGLISRE
                     DVDSIKKMILSLNMNLDFSVDIDLIKEAFFKDKKRESNDIFFVLLDAIGSAVVQKLTM
                     AEVEEYVETLCCGK"
     gene            complement(3864..4688)
                     /gene="aroF"
                     /locus_tag="P5123_02935"
     CDS             complement(3864..4688)
                     /gene="aroF"
                     /locus_tag="P5123_02935"
                     /EC_number="2.5.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006422823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003849 - 3-deoxy-7-phosphoheptulonate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0009423 - chorismate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-7-phosphoheptulonate synthase"
                     /protein_id="HRX15248.1"
                     /translation="MKNLKLASINHTEGERTIIKIGEHTIGKDFIIVGGPCSVENETQ
                     LMETAKAVKSAGAQMLRGGAFKPRTSPYDFQGLGLEGLRLLDKAKQKYDLPIVTEVVD
                     PRDVSWVCEFADILQIGARNMQNFSLLREVGLSGKPVILKRGMYSTLAEWVNCAEYIL
                     KEGNPNVILCERGIRTFETYTRNTLDLSIVPAAKEVTHLPVFVDPSHGTGKYSLIEPM
                     CYAAVAAGADGLLIEVHNCPEKALSDAKQQYTPEVFSHLVKKINKAVKCMRGLTDV"
     gene            complement(4851..7058)
                     /locus_tag="P5123_02940"
     CDS             complement(4851..7058)
                     /locus_tag="P5123_02940"
                     /inference="COORDINATES: protein motif:HMM:NF012544.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HRX15249.1"
                     /translation="MSDQKKFGTFAGVFTPAVLTILGVIMYLRLGWTVGSLGLGKAIV
                     VILLAHIVTLTTGLSLASLATNVRVGAGGFYAFISRSLGAEAGGAIGIPLYISQALSI
                     SFYIMGFSEAWQLLFPSHNHLIVLLSVLILMALLSFIGANSALKMQYIIMILIVLSIV
                     SFFLSPIKIDTEVQLFKKGVVNADFWFVFAVFFPAVTGIGSGVAMSGDLKNPKKSLPL
                     GILAAIGTGFVIYMTLAIWYAHIASAKKLTEDFYVMVEYSRFPILVILGLMGATVSSA
                     LGSLVGAPRILQALANDRVIPLSSIFSKMSKNGTPRMATLLTVMIVIGSLFFGDLDSI
                     AALLTMFFLITYAVINFTVFVEKLIRIPSFRPSFNVPMIIPAAGILWCTGVMFLINIY
                     FAITAYIVIVILYFIYVRRGLNAPFGDVRSGIFTALAEWAVRTSFRLPQHQKTWKPDL
                     IVPVQNDKQWSKRKEFVQAIVNPGGSIRLVSVNETDFHGKNVISSVINRYLKTSDKDK
                     RQYLKHFPGFEDRFLELDHIKKELMKEGVFSTATVVDAVSFLEGLSIVIQTSRGMFFP
                     PNSILLSMSDDPKKDVRLMEILAISVREKLGVILLNCNSETGFGDRKSVNIWLRVDSP
                     NKNLAVLTGLQLGKNWRCKLRLVSSVLNDQEYDHAVTQLKEVKELGRLHANTQITVIR
                     KSFREALDDVSRADLNIFGISQNVDIGFIRSISSSVSGSSLFIMDSGQERIDV"
     gene            7272..7700
                     /gene="fliS"
                     /locus_tag="P5123_02945"
     CDS             7272..7700
                     /gene="fliS"
                     /locus_tag="P5123_02945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015675765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009288 - bacterial-type flagellum
                     [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar export chaperone FliS"
                     /protein_id="HRX15250.1"
                     /translation="MALQSNPYDQYKKTQVNTASQGKLIVMLYDGAIKFLTIAAESME
                     PKTYDIVNHNIIRAQDIITELISSLNLDSGGEIANNLLSLYLYFKKRLLEANMQKDSK
                     ILEEVMKHLRELRDAWNEISNKESSNDNAGDRKGSFSIEG"
     gene            7690..8154
                     /locus_tag="P5123_02950"
     CDS             7690..8154
                     /locus_tag="P5123_02950"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRX15251.1"
                     /translation="MRDDIKKMFLDLNNCYTQKITHYKDLLRNCRECSFQVSSGNYSR
                     IEDLLEESNITITEIDNIDYQIAAIKTSICSKCGIEYYNFESYFFYKNDFAESSHTKV
                     LIDYMKPLLRQSIEQRDSLVKKIQVEQNILAGSIDSLSRTRAINTHFHLLQG"
     gene            complement(8179..9021)
                     /locus_tag="P5123_02955"
     CDS             complement(8179..9021)
                     /locus_tag="P5123_02955"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRX15252.1"
                     /translation="MSKKDDLIDDFTNLDYGFSRLKIDRNRSDGIFLNIFDAGGIRYL
                     MKKCGLHDHLKKKRFYPYTIIVFRDENDIHNLRILESERNVPLVDLRVSEKRFVKTGF
                     DGQTLSFDMVAIEWLSTRDPRLFTFKSNRPQLPGQTHPSLGCLSLMMKMMYLCSRRIH
                     KDAFMDVPDHLHLALMYSSFFHFLDPAAEAVIRAVKRGLKGYSLSEIAWGAVTGSIFD
                     KKTGELFVYKPSEQLYPVSRRMKRYFSSKPYQEAVKNSNPGQFTLNRDNLDKKIESAK
                     KKYL"
     gene            complement(9021..10163)
                     /gene="prfB"
                     /locus_tag="P5123_02960"
     CDS             complement(9021..10163)
                     /gene="prfB"
                     /locus_tag="P5123_02960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013176031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003747 - translation release factor
                     activity [Evidence IEA];
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 2"
                     /protein_id="HRX15253.1"
                     /translation="MFKVLEVNVGIEISDLLKKISVLATKASDTYNGIGIDGKKKQLE
                     KLESMTYTDGFWNDKENSAQVNKEISILKKKVDPWEQIITDIKDAKELLEVAAEEGDA
                     NLIEEITEQLKHFEERFENLENLELLSGEDDLNNAFITVHAGAGGTESQDWALMLLRM
                     YIRWAEKSGYDAETIDYMAGEEAGIKQATVLIKGDYAYGLLNCEAGVHRLVRISPFDS
                     NKRRHTSFASVEVHPEISEDIDIDIVEADLRIDTYRASGAGGQHVNTTDSAVRITHIP
                     SGIVVSCQNERSQHKNKASAMKVLKSKLYEKQKIEMQERIDEKAGEKKDIAWGSQIRS
                     YVFQPYTMVKDHRTGEETGSVEYVLDGDITKFIYAYLRFKNTQKSE"
     gene            complement(10363..11493)
                     /locus_tag="P5123_02965"
     CDS             complement(10363..11493)
                     /locus_tag="P5123_02965"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA photolyase"
                     /protein_id="HRX15254.1"
                     /translation="MIKSDSIKAVIYNNSEVNEYVDKFLNLQNVDYYCYDNDDELNRI
                     IANVSKKYESKEVIIFKKFLGRIFQMCPGTPEMICCNYRLVNTGFNCLYNCAYCYLQI
                     YLNSFGIQLFSNMDQLYSHIDPFFDQIKSDYIYRIGTGEFTDSLMFDDLTGIGKKLIE
                     IFASYPSIMLELKTKSSNIDHLLDIEKKGNSVIGFSINTDTNSKLYEEGAATVKERLI
                     AAAKAAKAGYYTAFHFDPVIIYDNWQEEYLAVVDMLFDYLPATSIAWISMGGFRYQGS
                     FIKTVRNNFPGEKMSTGEFFPCKDMKYRYQWHLRSKIYRTLYNAIRVKTDHPYVYMCM
                     ESSEMWKEVFGKNLETSEHLEKDFSDHLRNMLVMDDRLGIDN"
     gene            11553..11728
                     /gene="ssrS"
                     /locus_tag="P5123_02970"
     ncRNA           11553..11728
                     /ncRNA_class="other"
                     /gene="ssrS"
                     /locus_tag="P5123_02970"
                     /product="6S RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00013"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00013"
     gene            11920..14673
                     /locus_tag="P5123_02975"
     CDS             11920..14673
                     /locus_tag="P5123_02975"
                     /inference="COORDINATES: protein motif:HMM:NF015644.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UPF0182 family protein"
                     /protein_id="HRX15255.1"
                     /translation="MNKRISVTAAIVIFMIYVLLNTGSFITDYWWFDHYQNLKTFFTL
                     FITGLTVKLIFATIFLIIISINTFIVIRLSGGKHNAYSSVISYLPPPLQNTKYITIVI
                     FAVLVFLAYIMGSGAKPFWSEILIYFNSSHSSSLPVDPVFNRPVEFYFFSLPFYNFIH
                     GWFIGTLILISIVTALSYILFGGISFKFGRPAVSKPARAHLSVLGSLFMLIISAGYYL
                     SAFDILFTQRGKFFGAGYTAVHSQLFAYRACMILAIIASVLIIVSIFRKTIKFALLTA
                     GSTFALFVILGSIVPSFQQRFTVEPNELERERPFILNNIKFTRLAYGLSDITIREFEN
                     NGQLSREDIENNQDVLSNIRLWDWRPLKQTYNQLQGLKPYYSFMDVDVDRYEVNGKLS
                     AVNIAGRELLNSELAPNSQTWVNNHLVYTHGYGVVMNRVDRVTTEGQPDLIIKNIPPQ
                     SDFIDIKRPQIYYGEHNNDYIITNSEVNPGEFDYPSGDTNSYNTYEGTGGVILDSFFK
                     KLIFAVSFSDINILISNNISNKSRIHYFRNITKMAKRIAPFLEIDTDPYIIISDNGEL
                     YYIIDAYTISDGFPYSTPLNGSKTNYVRNSVKIVINAYDGNINFYINDDTDPIIKTYS
                     SIFPEIFSPLSKMPQDIKKHLRYPETMFDIQAQMLLRYHMTDANVFYNNEDMWELPKQ
                     IYDSSEQLMESYYLITKLPGETETEFVLMLPFTPFQKNNMLGFLIARCDNDKYGQMTL
                     YRLPKEKLSYGPMQLEARINQDSDISKQLSLWSQKGSRVIRGNMLAIPINESLLFVEP
                     LYLKAESSEMPELKQVIVCFRDSIVMEETLTKALEKTFGSTMSASEDSTSSELDTYTD
                     KDKDKVKSVISETVKTLSRQAYEIYTRAENRLRSGDFEGYGAAVSELGEVLKKLDDQA
                     KSE"
     gene            14806..16011
                     /locus_tag="P5123_02980"
     CDS             14806..16011
                     /locus_tag="P5123_02980"
                     /EC_number="2.7.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003739718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004618 - phosphoglycerate kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate kinase"
                     /protein_id="HRX15256.1"
                     /translation="MAIKNIEALKISGKKVLMRVDFNVPIKDGVITDDTRIRAALPSI
                     NYVLKNGGKAILISHLGRIKSEDDKKTKSLKPVAVKLSEHLGKEVKFVPFTRGAEVEK
                     AVSELKDGELMMIENTRFEDYADGQEVKKESKCDEELGKYWASLGDVFINDAFGTAHR
                     KHASNVGISQHIESAAGFLLDKEIKFIGGVVDDPQKPLIAILGGAKVSDKIGVIESLI
                     DKADKIIIGGGMMFTFLKAMGKNIGKSLLEEDKLDLAKSLMDKAKSKGVELILPVDTI
                     AAKEFKNDTDFRTVSCDDMANDEMGLDIGEASIKLFTEKLTGAKTVVWNGPMGVFEME
                     NYAQGTIGVCKAIASLDAITIIGGGDSAAAAEQLGFADKFSHISTGGGASLEYLEGKV
                     LPGIAALDK"
     gene            complement(16114..17592)
                     /locus_tag="P5123_02985"
     CDS             complement(16114..17592)
                     /locus_tag="P5123_02985"
                     /inference="COORDINATES: protein motif:HMM:NF012245.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein"
                     /protein_id="HRX15257.1"
                     /translation="MSLSNSTARTNRFVLTLNWILDSFLIIGYLGELIKGGRSLTFVL
                     VFITIILIPMITATLIFKKDNNSLSMRNVTLGGYFILYVFVLFSTERVLVYTYMFPII
                     SMYILYFNLKLITVSCVFIIVANVSRVIWLITFMGMRDSSITTDFTIQLASVFLYSFS
                     LIVSTKLSNKYNDEKMESINDYTSRQKEIIDDILHIAKIIDVNSEQVKVIVDELAETA
                     GQVRAAVNEIAMGADTTSDSIQKQSELTTTIHRTIENTSRLAEDLRNISSLTVETVND
                     GKKIVQQLSANGKTVERESRYSVESMSALKDKSNEIQSISKMISDISEQTNLLSLNAA
                     IEAARAGESGRGFAVVADEISKLAEQSKMSAGEINHIVSDLQTTADRSLEAVDNLTRV
                     NSEHSVYVQNTENILNSITQKMDEVNATVILVEKRINEILGSNDQIVESIMSISQVAE
                     ETSASSQLTTDMTKQNIHRSQKATELVSELMESSKMMDKYIK"
     gene            complement(17950..>19248)
                     /locus_tag="P5123_02990"
     CDS             complement(17950..>19248)
                     /locus_tag="P5123_02990"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_component: GO:0098561 - methyl accepting chemotaxis
                     protein complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein"
                     /protein_id="HRX15258.1"
                     /translation="NASIVSSKYSNTSGVFDILANKLNEMSNFINQNLEIIVSVVRPI
                     TEGVQMLIKANENVLFEIGEGIKSLDELNEVLDKQIESIKELFTRAEISGKEIKGQKD
                     MLSEVSQMIVHMDSDAQQAIQGSANVADLARNLQEEVTHFLSIHNYNDDFIDRVNVTK
                     EKALRIWQNAENVNNRSKNQLEFSKNCQNFNEKIIAESKQLKETAEILHKQTGNNSKV
                     SEDISANLTRLTQQLNDIEKNMNNSSRTIQKFNDDYKQIDNILEFLKNILKSMHLIGM
                     YSRIESARDVEEFSGFMNISANITNLQKEIQNNIPVIEKNITDTHQLIDSVNLAFKNI
                     SSDFDIISGSSDQIISHLEDIVSVNRRTETTSSDMLENSSRIITFLNTLDDYLIDLMK
                     VVEFPIEGSLQNIERGKRMENIFTDIVKDVQNMLASSVSE"
BASE COUNT         5997 a         3995 c         3239 g         6017 t
ORIGIN      
        1 acagcggcgg agtccagagc ggttgcaatg ctgaagccga atattgttgt tgcggaacct
       61 accgatttga tcggaacggg catgacaagc gatgacgctt atattgaaac atcgattcgc
      121 gcggtgaagg aaatcgaccc caatatcttt gtattggcag cggccggaat cagcagcggc
      181 gcagacgttt accgtgtcat tcgcgcgggc gctgaagcaa cagggtcttc cagcggcatt
      241 acgctggccg aagaccgcgc ggcgatgata gacgagatga ttggagcggc tcgcagagca
      301 tgggatgagc gcgccactgc ccgttaagca aaggctgcat gataagatag tgaaagtatc
      361 tcaatagtca tatctatata aggcttgctt gtcagattat tcacaattat tctggatgat
      421 ttatcacaat gagcataagc gattaaaagc ccagtaatca actgcgaaga aacactgccg
      481 tcaacagtaa tttcattatt actaacaggc ccttcaatgc acattggcaa aaaaccgttt
      541 tgtgttgaac atgaaactcc catctttatt agagcatctt caaccatatc tattggacgt
      601 tttaaaagag aaccttgacc tgtaaaaaca aacttgtgat tctgtaaggc tgctattgga
      661 gcaaacattc ttactgttaa tcctgcttct ccgcaatcaa cagtatcaga ggctttacgg
      721 aatcttggtg taattaacgc agatgagcca tcaagctcga cctttgttcc tagagcctct
      781 gcggctctta tcgaagcaaa aagatccgcg caaccactta atccgtttaa cctgcagcta
      841 cttttaccta atgatgctat agcgacagct cgttgcatca tactctttga cgaaggcgcc
      901 cgaacagttc cctcaacaga tgatctgttc acaaatctct tcatttatcc tccgtacact
      961 ttgattaacg tctttatcac tattaataat tgtcatatca gcacatccgg catatagttc
     1021 tttacgagat tcaaaaagag ccttaaattg ctcggcacca tctgaagcaa gaggacggtt
     1081 agtattagcc accctttcaa gagatttatc catgtcggca tatatccaga tattcaaaca
     1141 ttttttgcgc aaaatatctc tgtttgcttt ccttgtgaca acaccgccac cgcatgatat
     1201 tatctttttt tcattatttt cacaaagctt ttcgagcagt tcacactcct tttgacgaaa
     1261 ccactcctcc ccctgtgttt taaatatatc ggcaatggat acgccctcac tattttctat
     1321 aatttcatcc atatcataac agggtatttc cgtacaaaat gccagattct ttgcaataga
     1381 acttttacca gatcccataa atcctgttaa agtgatcgta cctgttttaa tttttacagg
     1441 ttccataaac gattcaggtg gcttaatatc gccaaaacgt ttaccaaaaa aatgctcggc
     1501 cgcgcgatca gcttgataat agagccactc ttctccacga acataacgca caccggcctt
     1561 catcgccttt tgttgaaaaa cagactcctt gtatattgcg tccaaagcca gaacatcctc
     1621 tttaaaccac tcacttttaa taatgtcaac ttcgggtaaa agagtattta ttacaacatc
     1681 cgccttacgt attgcctctt cagcattatc cggtgtaaca gcagtaccgc cacaaagatt
     1741 tgctgccgaa acagctttct cgaatgtgcg attcaccaca gttactctga gcccaagtct
     1801 atccaatgcc acagcagcag ctcttgccgc tccccctgcg ccaagaagta cggctcgttt
     1861 atccttatat tttttaccac aatttaacaa cgtatagtat acaccgtatg aatcagttga
     1921 aaaaccagtt aaaacaccat tacggttttt tatggtattt accgactgaa gtcgtaaagc
     1981 ctcatcatct acacgatcca aaaaaggaat aacctgatct ttgaagggtg ctgttacgtt
     2041 acaaccagcg attcctgatg aacgtaaaaa agatataatt tcatgcccgc tttctgcagc
     2101 aatacgagta tatacatcag atgacccaca actcttaaaa tagttattaa agatcaacgg
     2161 gcttttagaa tggagcaccg gtttaccgca tacggcataa atcatctata aatctccaat
     2221 cagatctttg atcgactgca tctgagagaa tgtaagctga ccaggcgcag tcgttcgacc
     2281 actatccgga gccacaaaac taaaaggagc tcccagataa agcgacgcta ctcttgttat
     2341 ttttccgtaa tctcccatac ccaatattat catctcttct tcacgctgat agagagccaa
     2401 aagtctcgct gcatcttttt gggtccttgc caaacatgca atctttacta tatcagcacc
     2461 atagcttatt gatttatcga taatttcatt taattcgcga ttatcgggag tcagctggta
     2521 gttatgataa gaaattatta ccttgcatcc gaccaaacgt gcagcatcaa ttatgggagc
     2581 gttaaattca cggctgtttt ctatttccag atcaacataa taggcgcctg ccttaaccgc
     2641 agtgataagc atattttgtc tgaactcttc ttctactttt cctggcctgt atgttacaac
     2701 taacttcctc tttgcagatg agaagatctc atttatctct ttttcagaaa aagacgcgta
     2761 atcaaggcga atctcggata tatcagccga tgaacactcc ctgacaatat cttcaatact
     2821 atcttcactt accgcaacac agagtttcga catactcctc gacctccgcc attgttaact
     2881 tctgaaccac agcgcttcct atagcatcaa gaagcacaaa gaatatatcg ttcgattcac
     2941 gcttcttatc cttgaaaaac gcctctttta ttaaatcaat atcgaccgaa aaatcaagat
     3001 tcatattcag cgataatatc atttttttta ttgaatctac atcttcacgc gaaataagcc
     3061 ctcttataca tgaaatttgg gccgcaaact gcatgccctg tgcgacggca agaccatgat
     3121 caattttaat gcatttttca agcgcatgtg caagagtatg gccaaaattc agtatacgtc
     3181 gacgtccttg ttcacgctcg tcttctgtca cgacttcggt tttgatttct atagatctta
     3241 taatcagctg gcgaagaatc tttttatccc gttcgtttat taaatcaata ttttcgttta
     3301 ttaaatcgta catattgttg tcagcaataa gagcatgctt gacaacttct ccaagtccat
     3361 ttatatactc tttgtcagga agagtatcca acatttcgat atcacagata acaaaatcgg
     3421 gctgattaaa aacaccaacc atgtttttat aaccatgatg attaacaccg tttttcccac
     3481 cgacacttgc atccacttga gacagtaaag tagttgaaac aaatccaaag cctactcccc
     3541 gcaaaaatgt actggcaaca aaaccagcag tatctgtaac tataccaccg ccaattccaa
     3601 caatatatga agagcggtcg caaccaagtt caataagctc attatgaagc tgtgcaacag
     3661 tatcaatagt tttatgttcc tctccgcact ccattacaag aacgggacaa tcaggaaact
     3721 gatccttata gtacatgtac acattggaat ctgtgataat tacgcttttt tttaacggaa
     3781 tataattcga caaagaagac aaagattcgc caacaagtac ttttgatgaa ttaccggaag
     3841 ttttgtagtc gattctgaag atatcagaca tctgtaagcc cccgcatgca ttttacggcc
     3901 ttattaattt tttttacaag atgagaaaaa acttcaggcg tatattgttg cttagcatct
     3961 gacagagctt tttccggaca gttgtgaacc tcgatcaaaa gtccatccgc accggcggcc
     4021 acagcagcat aacacatcgg ctcaattaat gagtacttgc cagtaccgtg tgaaggatct
     4081 acaaaaaccg gaagatgggt tacctcttta gccgcaggta caatgcttaa atccaaagtg
     4141 ttacgggtat aggtttcaaa agtacgaata ccccgttcac agagaattac attcggatta
     4201 ccctctttaa gaatatattc agcacagtta acccactccg ccaaggttga atacatacct
     4261 cgcttaagaa ttacaggttt tcccgaaagg ccaacttcgc gaagaagcga gaagttttgc
     4321 atatttctcg cgccaatttg caatatatcg gcaaactcgc aaacccatga gacatctcgc
     4381 ggatctacaa cctcggttac aattggaaga tcatactttt gtttagcctt atcgagtaaa
     4441 cgcaaaccct caagcccgag cccttggaaa tcgtaaggag atgttcgggg tttaaaagcg
     4501 cctccacgaa gcatttgtgc tccggcactc tttacggctt ttgccgtttc cattaattgt
     4561 gtctcgtttt cgaccgaaca tggacctcca acaattataa aatccttgcc aatagtatgc
     4621 tcgcctattt taattattgt tctctcaccc tcggtgtgat tgatagaagc caatttaaga
     4681 tttttcataa tacgccctta gacagataca acagattatt ccgtagtctg cattataact
     4741 tttcatgcta ctttttatcc ttacaaaaat gagtcaacat tatttttaaa ctgttcattt
     4801 ttaacataat tattcgttcc cagcttacca tgaacatcta atttatttgg ttaaacatct
     4861 attcgctctt gtcctgaatc cattataaaa agagaactcc ctgaaaccga agagcttatg
     4921 cttcgtataa aaccgatatc cacattttgt gatataccga agatattaag atctgccctg
     4981 gatacatcat caagagcttc tctaaacgat tttcttatta cggttatctg ggtattggca
     5041 tgaagccgac ctagctcctt aacctctttt aattgagtga cagcatgatc gtattcttga
     5101 tcgtttaaaa ccgaacttac tagtcggagc ttacatctcc agttttttcc taattgcaaa
     5161 cccgttaaca cggccaaatt cttgttaggc gaatccactc gaagccatat attcacgctc
     5221 tttcgatccc caaaccctgt ttccgaatta cagttaagca gaatgactcc tagtttttct
     5281 ctaacagaaa tcgccaaaat ttccatcaat cgcacatctt tttttggatc gtcactcatt
     5341 gatagaagaa tactgttcgg agggaaaaac attccacgtg atgtctgtat tacaatactt
     5401 agaccttcca gaaaactcac agcatctacc acagttgccg tcgaaaaaac tccctctttc
     5461 ataagctctt tttttatatg gtccaactct aaaaaacgat cttcaaaacc tgggaaatgt
     5521 ttaagatatt gtcgcttatc cttgtcagaa gttttcagat accggttaat aacagatgat
     5581 atcacgttct taccatgaaa atcagtttca tttactgata caagtcgtat cgaaccacca
     5641 ggattaacta ttgcctgtac aaattccttt ctttttgacc actgtttatc attttgaaca
     5701 ggtacaatca gatcaggctt ccaagtcttt tgatgctgcg gtaatcgaaa tgaggttctt
     5761 actgcccact ccgctaaagc cgtgaatatt ccacttcgaa catcaccgaa aggggcattt
     5821 aaccccctgc gcacataaat aaaatatagt ataactatta caatatacgc cgttatggca
     5881 aaatagatat ttataaggaa catgacacct gtacaccata aaattcccgc tgcgggaatt
     5941 atcataggca cattaaaaga tggtctgaaa gagggaattc ttatcaattt ttccacaaaa
     6001 acagtgaagt taattacagc gtatgtaatc aaaaaaaaca ttgttaataa tgcggctatg
     6061 gaatccagat caccaaaaaa aagtgatcca ataacaatca tgaccgttaa taatgttgcc
     6121 attcgcggtg ttccgttttt actcattttt gaaaaaattg atgataatgg aatcacacga
     6181 tcattggcaa gtgcctgaag aattcttgga gccccaacca gagaacctaa tgcagacgaa
     6241 acggttgctc ccataagccc taaaataaca agtatgggga aacgtgagta ttcaaccatt
     6301 acataaaaat cttcggtaag ttttttagca gacgcaatat gagcatacca tattgccagg
     6361 gtcatataaa taacaaaacc tgttccaatt gccgccaaga ttcccagtgg aaggctcttt
     6421 tttggatttt tcaagtcacc agacatcgct acacctgaac cgatacctgt aacagcaggg
     6481 aaaaaaaccg caaagacaaa ccaaaaatca gcattaacaa cacccttttt aaacagttga
     6541 acttcggtat ctattttaat tggagatagg aaaaaggata ctatcgacaa tactatcaaa
     6601 atcattatta tatactgcat cttaagagca gaattagccc caatgaacga aagcagagcc
     6661 attaaaatca gaactgacaa tagtacaata aggtgattat gtgacgggaa aagcagttgc
     6721 caagcttcag agaaacccat tatataaaaa gatatgctca gagcctgtga tatgtataag
     6781 ggtatgccaa tagcccctcc agcctctgca ccaagagagc gtgatataaa agcgtaaaac
     6841 cccccagccc ccactcttac attagtagcc agtgatgcca aagataagcc ggtagtcaaa
     6901 gtaactatat gcgccaacaa gatcacaact atagccttgc caagcccaag cgatcctacg
     6961 gtccagccaa ggcgtaaata cataataacg cccagaattg tcaaaacagc cggagtaaat
     7021 accccggcaa aggttccaaa ttttttctga tccgacatgc aaatgctcct ttaacatgat
     7081 acaatcagac cttataatga ttttttttca atcaataaac aatttaacca gtaaaactaa
     7141 atatccttcg tatatcattg tatggttttt cgcatatttt ttattttttc ttattataat
     7201 gaaaaaaaac ttaagtcgct atcattttta accgattctc aagataagga tttattgacc
     7261 gggaggattg gatggcgctt cagagtaatc cgtacgatca gtacaaaaag acacaagtta
     7321 atacggcgag tcagggcaaa ctcatcgtaa tgctctatga tggagcaata aagtttttaa
     7381 caattgccgc cgaaagcatg gaaccaaaga cttacgacat tgttaaccac aatattatcc
     7441 gcgcacaaga catcatcacc gagcttatct cttcattaaa tcttgattca ggtggtgaaa
     7501 tagcaaataa tcttcttagc ctctatcttt actttaaaaa aaggcttctt gaagcaaaca
     7561 tgcaaaaaga tagcaaaatt ttggaagagg ttatgaagca tctcagggaa cttcgcgacg
     7621 catggaacga aatctcaaat aaagagagtt caaatgacaa cgcgggtgac cggaaaggga
     7681 gtttttcaat tgagggatga tattaaaaaa atgtttcttg atttaaacaa ctgctacaca
     7741 caaaagatta cccattacaa agatcttttg cgaaactgca gagagtgctc atttcaagta
     7801 agttccggaa actatagcag aatagaagat ctgcttgaag agagcaatat aactattacc
     7861 gaaattgaca atatcgatta tcagattgct gccataaaga catcaatatg ttctaaatgc
     7921 ggcatagagt actataattt tgagagctac tttttttaca aaaatgattt cgcggagagc
     7981 agtcatacca aggttttaat agattatatg aaacccttgt taaggcaaag catcgagcaa
     8041 cgagattcgc ttgtaaagaa gattcaggtt gaacagaata ttttagcggg ttcaattgat
     8101 tcgttatcac gtacacgagc cataaatacg cactttcatc ttttacaggg atagcatatc
     8161 tcctgaaatt gaattccgtt ataagtattt ttttttcgcg gattctatct ttttatctag
     8221 attatcccgg tttagtgtaa actgaccggg attactattt tttacggcct cctgatatgg
     8281 ttttgaagag aagtatctct tcattcttct tgaaaccggg tacagctgct cggaaggttt
     8341 atatacaaaa agctcacctg tttttttatc aaaaattgat cccgttactg cgccccaggc
     8401 aatttctgaa agactataac ctttcaatcc tctcttaaca gctcttatta cagcttccgc
     8461 agcgggatca agaaaatgaa agaaactcga atacataaga gctagatgaa gatgatctgg
     8521 tacatccata aatgcgtcct tatgtattcg ccgtgagcac aaatacatca tcttcatcat
     8581 aagagaaaga cagcctagtg aaggatgagt ctgccccggt aactgcggcc gatttgactt
     8641 aaaagtgaag agtcttggat cacgagttga aagccattcg atggcgacca tatcaaaaga
     8701 caaggtttgt ccgtcaaaac ctgttttgac aaatcttttc tcggatacgc gtaaatctac
     8761 cagtggtaca tttcgctcgc tctctagaat tcgcaagttg tgaatatcat tttcatctcg
     8821 aaaaacaatt atggtatagg gatagaacct cttttttttt agatggtcat gcaaaccgca
     8881 ttttttcatc agatatcgaa taccacccgc gtcaaaaata ttcaaaaata ttccgtcact
     8941 acggttacgg tcaattttta acctcgaaaa tccatagtct aggttggtaa aatcgtctat
     9001 caagtcatct tttttactca ttattcactc ttctgggtat ttttaaaacg cagataagca
     9061 tatataaatt ttgtaatatc accatcaagc acatattcca cacttcccgt ctcttcgcct
     9121 gtacggtgat cttttaccat ggtataaggc tgaaaaacat aggagcgtat ctgacttccc
     9181 catgcaatat cctttttttc accggccttt tcatctatac gttcctgcat ctcaatcttt
     9241 tgcttctcgt atagctttga ttttaaaacc ttcattgccg acgccttgtt cttatgctga
     9301 gacctctcgt tttgacaaga aacaacaatt ccagatggaa tatgagttat ccttacggca
     9361 gagtcggtcg tattaacatg ttgaccacct gcccctgaag cgcggtatgt atctattcgc
     9421 aaatcagctt caactatatc gatatcaata tcttcagata tttcaggatg cacctcaaca
     9481 gaggcaaacg atgtatgtct tcttttattc gaatcaaacg gtgaaatacg aacaagccga
     9541 tgtaccccag cttcacaatt taatagtccg tatgcatagt caccttttat taaaacagtt
     9601 gcctgtttaa ttccagcctc ttcacccgcc atataatcga tggtttccgc atcatatcct
     9661 gatttctcgg cccaacgaat atacatgcgt aaaagcatta atgcccaatc ctgtgattcc
     9721 gtaccaccag cacctgcgtg tacggttatg aaagcattgt ttaagtcatc ttcccccgat
     9781 aataattcaa gattttcaag attctcaaaa cgctcttcga agtgttttag ctgctcggta
     9841 atttcttcta tcaagttagc atcaccctct tccgcggcaa cttcaagcaa ctcctttgcg
     9901 tccttaatgt cagtaattat ctgttcccaa ggatcaactt tttttttcag gatactgatc
     9961 tctttattta cctgagcact gttttctttg tcgttccaga acccatcggt atatgtcatg
    10021 ctctcaagtt tttcaagctg cttcttctta ccgtcaattc cgattccatt ataggtgtct
    10081 gaagcttttg tagcaagaac tgatattttt tttaataaat cactgatttc aatacccaca
    10141 ttaacctcta gtactttaaa tatttgtatt tttcaataat agatatatct atcattatta
    10201 aatatatttt tcattattag atatatttat caatacatac acgaataaga tcatataaac
    10261 attaccgaaa tatagattat ttttaaatct tgaatattcc tgtaattttc gaattcaata
    10321 tttctattta taaactgtac ccgtaaaagt ctttaagcaa atttaattat ctattcctag
    10381 tctatcatcc atgacaagca tattccgcag atgatccgaa aaatcttttt ctagatgttc
    10441 agatgtctct aaattctttc cgaacacctc tttccacatc tctgaactct ccatgcacat
    10501 ataaacataa ggatgatcag tttttacccg tatggcattg taaagagttc tataaatctt
    10561 agaacgtaaa tgccactgat acctatattt catatcttta catggaaaaa attcgcccgt
    10621 actcatcttc tcacccggaa aattattccg tactgttttt ataaaagagc cctgataacg
    10681 gaacccaccc atggatatcc atgctatcga agttgccggc agataatcaa aaagcatatc
    10741 gacaacagct agatactctt cttgccaatt atcataaata attaccggat caaaatgaaa
    10801 cgccgtataa taacctgctt ttgccgcttt tgcggctgct attagcctct ccttgactgt
    10861 tgcggcaccc tcttcataaa gctttgaatt agtatccgtg tttatgctaa agcctattac
    10921 gctatttcct tttttttcaa tatcaagaag atgatctata ttcgaagatt tcgttttcag
    10981 ttcaagcata atcgatggat aagaagcgaa aatctcaatc aattttttac caattccagt
    11041 taagtcatca aacattagtg aatcggtaaa ttcaccagtc cctatcctat aaatatagtc
    11101 actttttatc tgatcgaaaa aaggatcaat atgactgtaa agctgatcca tatttgaaaa
    11161 aagctgtata ccaaatgaat ttagataaat ctgcaagtag cagtaagcac aattatacag
    11221 gcagttaaat ccggtattta ccaaacgata gttacaacat atcatttccg gagttccagg
    11281 gcacatttga aatattctgc ctaaaaattt tttaaaaatt atcacttctt tggattcata
    11341 ttttttactg acatttgcaa tgattctatt taactcatca tcattgtcat aacaatagta
    11401 atcgacattc tgcaaattca aaaacttgtc aacatattca tttacctcag aattattata
    11461 gattacagct tttatactat cagatttaat catctttact ccagcaaata taaaattttc
    11521 ttgacgacta tttacaggct ggtctattga ctccaagcct gcgttgttcg tgttaggatt
    11581 aatgcccttt aacctattta agctcaggga tctcaatatt cagatctgta ggccatgccc
    11641 gaaagggact gctgaattgt ctgtgaggga acccacctgt actacaggtt aagagcggct
    11701 tttttctcac ggcaaacgcg ggtttttaac aacatacctt tgaactaatt aatttcatag
    11761 aaatatatcc cgccgtaaac aggctttatg tctactgttt ttttcttgcc gtttaaggat
    11821 actttatctt atttctgaat atcagggttg acttatcata gttaataatg ttaacctatc
    11881 gatattatta acatcaactt ataccttatg gagcaatcta tgaataaacg tatttcagta
    11941 acagcagcta ttgtcatttt tatgatatat gttcttctta acactggatc ttttattaca
    12001 gattattggt ggtttgatca ttaccaaaac cttaaaacat tctttactct ttttattaca
    12061 ggtctcacag ttaaactgat atttgcgaca atttttctaa taattatttc gattaatact
    12121 tttatagtaa taagactctc agggggaaaa cataacgctt attcttctgt aattagctat
    12181 cttcctcccc ccctacaaaa cactaagtac attactattg taatatttgc tgttttagtt
    12241 tttcttgcat acattatggg atcgggagct aaaccattct ggagcgaaat actgatctat
    12301 tttaatagtt cacactccag tagccttccg gttgatcccg tgttcaatcg accggttgaa
    12361 ttttactttt tttctctacc tttttataat tttattcatg gttggtttat aggaacttta
    12421 attcttattt caatcgttac cgctttatct tatattcttt tcggtggtat cagttttaaa
    12481 tttggacggc ccgctgtttc aaaaccggca agagctcatc tatctgttct tggttcgctt
    12541 tttatgctta ttatttcggc aggttactac ctttcggctt ttgacatact gttcacacaa
    12601 agaggaaaat ttttcggagc cggttataca gctgttcatt cccagctatt tgcctaccga
    12661 gcctgtatga tccttgctat aatagcttcg gttctaatta ttgtctccat attcagaaaa
    12721 accataaaat ttgcgctttt gactgccggc tcgacctttg cacttttcgt cattttagga
    12781 tctattgtac catcttttca acaacgtttt accgttgaac ccaacgagtt agaacgtgaa
    12841 cgccccttta ttttaaacaa tattaaattt acaagacttg cctatggttt atcagatatt
    12901 actatccgtg aatttgagaa taacggtcaa ttatcaagag aagatataga aaataaccag
    12961 gacgttcttt cgaatatcag gctttgggac tggaggccat taaaacagac atataaccag
    13021 ttacagggct taaaacctta ctactctttt atggatgttg atgttgaccg ctatgaagta
    13081 aacggaaaac ttagtgccgt gaatattgcc ggacgtgagc ttttaaattc agaacttgcc
    13141 cccaattctc aaacgtgggt caataatcat cttgtatata cccatggtta cggtgtggtt
    13201 atgaaccgag ttgatagagt aacaaccgaa ggacaacccg accttattat aaaaaatata
    13261 ccaccacaat ctgattttat agatattaag cgcccgcaaa tttattatgg cgagcataat
    13321 aatgactata ttattaccaa ctccgaagtg aatcccggag aatttgacta tccatcgggt
    13381 gatacaaaca gttataacac ctacgaagga actggtggcg tgattcttga ttcttttttc
    13441 aagaaactta tttttgccgt atctttttca gatattaaca tattaatatc taacaatata
    13501 tcgaataaat ccagaataca ctattttcgt aatataacaa aaatggccaa acgtatagct
    13561 cctttcttgg aaatagatac cgatccttat ataataattt cggataatgg tgaactatat
    13621 tacataattg atgcctacac tattagtgat ggttttccat actccactcc acttaacggg
    13681 agtaaaacaa actatgtgag aaactcggtt aaaatcgtta ttaacgctta cgatggaaat
    13741 ataaatttct atataaatga tgataccgat ccaataatta aaacctattc ctctattttt
    13801 ccggaaattt tttcaccttt atcaaaaatg ccacaagata ttaaaaagca tcttagatat
    13861 cctgaaacta tgttcgatat tcaggcacag atgttattac gttatcatat gaccgatgcc
    13921 aatgtttttt ataacaatga ggacatgtgg gaacttccca aacagatata cgatagtagc
    13981 gaacaactta tggaaagcta ttatctaata acaaaacttc ccggagaaac cgagaccgag
    14041 tttgttctta tgcttccatt cacaccattt caaaaaaata atatgttggg ctttctcatc
    14101 gccagatgtg acaacgataa atacggacag atgacacttt acagacttcc caaagagaaa
    14161 ctaagctacg gcccaatgca gcttgaagca agaataaacc aggattcaga tatatccaaa
    14221 cagctttccc tatggagcca aaaaggatct cgtgttattc gtggcaacat gcttgcaatc
    14281 cctataaacg aatcgctact ttttgtggaa ccactctatc ttaaagcaga aagcagtgaa
    14341 atgccagaac taaaacaggt aattgtctgt ttcagagata gtatagtgat ggaagagaca
    14401 cttacgaaag ctcttgaaaa aactttcggc tctacaatga gtgcgtccga agactccaca
    14461 tcatcagaat tagacacata cacagataaa gataaagata aagttaagtc agtaatttcg
    14521 gaaaccgtta aaaccttgag ccgtcaggcc tatgaaattt acactagagc cgaaaacagg
    14581 cttcgttcgg gtgactttga aggatacggt gctgctgtgt cagagcttgg tgaagtttta
    14641 aagaaacttg atgatcaggc caaaagcgag tagttttttt gtgaaaaata tcacttgact
    14701 tatatatgca ttaattcggt ttaatagttt caagagaatg tgatgcattt tcttaagaat
    14761 attccaatgc cggcttaaaa agcttggcta taaagaggtt aaccaatggc tataaaaaat
    14821 attgaggcgc taaaaatttc aggaaaaaaa gtactgatgc gtgttgattt taatgtgcct
    14881 atcaaagacg gagtcattac agatgataca cgtattcgcg cagcccttcc ttccattaac
    14941 tatgtgttaa aaaatggcgg caaggctatc cttatttcac atttgggtcg tataaaaagt
    15001 gaagatgaca aaaaaacaaa atctttaaaa cctgttgcag tgaaactttc cgagcatctg
    15061 ggtaaagaag ttaaatttgt ccccttcacg aggggagctg aagttgaaaa agctgtcagc
    15121 gaattgaaag acggcgaact tatgatgatc gaaaacacac gttttgaaga ttacgccgat
    15181 ggacaagaag taaaaaaaga gtctaaatgc gatgaagaac ttggtaaata ctgggcctct
    15241 ttaggggatg tttttataaa tgatgccttt ggaactgcgc accgtaagca tgcttctaat
    15301 gtaggtattt cacaacatat cgaatcagct gccggatttc ttcttgataa agagattaaa
    15361 tttataggtg gagttgttga tgacccacaa aaacctctaa tcgctattct cggtggagct
    15421 aaggtctctg acaaaatagg tgtcattgag agcctgattg acaaggctga taaaattatt
    15481 atcggtggcg gaatgatgtt cacttttctt aaagccatgg gtaaaaatat aggtaaatca
    15541 cttcttgaag aagacaaact tgatcttgcc aaatcattaa tggataaggc aaagtcaaag
    15601 ggtgtagagc ttatacttcc tgttgataca atcgcggcta aagaatttaa aaacgataca
    15661 gattttagaa cagtatcatg tgatgatatg gcaaatgatg agatgggtct tgatatcgga
    15721 gaagcttcca taaaactttt cactgaaaag cttaccggag ccaagaccgt tgtctggaac
    15781 ggacctatgg gtgttttcga aatggagaac tatgctcagg gtacaatcgg agtttgtaaa
    15841 gcaatagctt cgctcgatgc cattaccatt attggtggtg gagattctgc agccgcagca
    15901 gaacagcttg gttttgcgga taaattttct catatttcga ccggaggcgg tgcttcactt
    15961 gaatatcttg aaggcaaagt tcttccgggt attgctgctc tagataagta atctttttta
    16021 ctacagaaaa tataaaaata agactgccat caggcagtct tatttttata tttataactt
    16081 cgactaattt ctcacttttc gactattatt tctttattta atatatttat ccatcatctt
    16141 agaagactcc atcaattcac ttacaagctc tgtagctttt tgagacctgt gaatattctg
    16201 ctttgtcata tctgtagtaa gttgggaaga cgcagaggtc tcttcggcaa cctgcgatat
    16261 actcataatg ctttctacaa tttggtcgtt tgaccccagt atctcattta ttcttttttc
    16321 tacaagtatt actgtcgcat ttacttcatc cattttttgc gtaattgaat ttaatatgtt
    16381 ttccgtgttt tgtacataaa ccgaatgttc actgtttact cgtgttaaat tatcaacagc
    16441 ctcaagtgac ctatcggctg ttgtttgcaa atcgcttact atatgattaa tctcaccggc
    16501 agacatctta gactgctcag ccaactttga tatctcatcg gctacaacag caaaaccacg
    16561 accggattca ccagcccgag ccgcctcgat ggctgcattc agcgacagaa gatttgtctg
    16621 ttcagaaata tccgatatca tttttgatat actttgtatc tcatttgatt tatcttttaa
    16681 agcactcata gattcaacac tgtaacgact ttctcgctcg acagtttttc cgttagcact
    16741 aagttgctgc acaatttttt taccatcatt tactgtttca accgttaatg atgagatatt
    16801 tctaagatcc tctgccaaac gtgacgtatt ttcgattgta cggtgtattg ttgtagttag
    16861 ctcggactgt ttttgaatac tgtcacttgt cgtatctgca cccattgcta tctcgtttac
    16921 tgcggctctt acttgccctg cagtttctgc aagctcatca acaataacct taacctgttc
    16981 tgaattaaca tcaattattt ttgcgatatg aagaatatca tcaataatct ctttctgtcg
    17041 tgaagtgtaa tcattgatac tttccatctt ctcatcgtta tacttattgg acagttttgt
    17101 tgagactatc aaagagaaac tatacaaaaa cactgaagca agttgtattg taaaatcggt
    17161 agttatagag ctgtctcgca ttcccataaa ggtaattaac cagattaccc ttgaaacatt
    17221 tgcaacaata ataaatacac agcttactgt aataagcttt aagttaaaat agaggatata
    17281 catcgaaata atcgggaaca tgtatgtata aacaagaact ctttcggtcg agaaaagaac
    17341 aaatacatag agtataaagt atcctcccag cgtcacattt ctcatggaca gggagttatt
    17401 atctttttta aatatcaaag ttgcggtgat cattggaatc agaataattg ttataaatac
    17461 cagaacaaaa gttaggctgc gcccaccctt tatcagttcg cctaaatagc ctattatcag
    17521 aaaactgtca agaatccagt ttagtgttaa aacgaaacgg ttggttcttg ccgtcgaatt
    17581 tgaaagggac atacatgctc catgcaaaag agtaataaca ttacacacaa aacaataact
    17641 tataagaatg catgataaat tatcggcatt ttgtaactct ttaataaaat atcaaagaat
    17701 aataataaaa aataatgtcg caaataaata cgtaataaca acctaatcaa gagaaaaggc
    17761 tatttcaact tgtgaacttc gaaaagaaat tattctaaca aaatatgaag ggcaccactc
    17821 gggaaacgga taacaatttt agatgtcagt ggaagacgat tcaaaattag caacagcttg
    17881 aatcaagctc accttttatc ttaaccgatt aatatcttat tttttggtct gttcagtgca
    17941 aaaaatattt tactcactaa cactgctagc caacatattt tgaacatctt tgactatatc
    18001 ggtaaagatg ttttccatcc ttttgcctct ttcaatattt tgcaacgatc cctctattgg
    18061 aaactctaca actttcatca gatcgattaa gtaatcgtca agggtattca aaaaagtaat
    18121 gattctggaa gaattttcaa gcatatctga acttgtagtt tcagtacgcc tattcactga
    18181 tacaatatct tccagatgcg aaataatctg gtcagagctt cctgatatta tatcaaagtc
    18241 tgaagagata ttcttgaaag caagatttac agagtcaata agctggtgag tatcagtaat
    18301 atttttttca ataacaggaa tattgttttg aatttctttt tgaagatttg ttatgttagc
    18361 tgaaatattc ataaatcctg aaaactcttc tacatcacga gcagattcga tgcgagaata
    18421 cattccaata aggtgcatcg actttaaaat attttttaaa aactcaagaa tattgtctat
    18481 ctgcttgtaa tcatcgttaa atttttgaat agtacggcta gaattattca tgttcttttc
    18541 gatatcattc agctgctgtg tcaagcgtgt aaggttagca gaaatatctt cggaaacctt
    18601 actgttatta ccggtctgtt tgtgtaaaat ttctgctgtc tctttaagtt gttttgattc
    18661 agcaataatc ttttcattga agttttgaca gttcttagag aattccagct ggtttttgga
    18721 acgattattt acattttcag cgttttgcca aatacgtagc gccttctctt ttgtaacgtt
    18781 aacccggtca ataaagtcat cgttataatt atgtatagat aaaaaatgtg taacctcttc
    18841 ctgaagatta cgtgcaaggt ctgctacatt tgcagagccc tgtatggcct gttgagcatc
    18901 gctatccata tgaacaatca tctgactaac ctcggataac atatctttct ggcccttaat
    18961 ctctttacct gatatttccg ctctggtaaa aagttcttta atcgattcga tctgcttatc
    19021 caaaacctca ttaagttcat ccagactctt aattccttca ccaatttcaa ataatacatt
    19081 ttcgttggcc ttaataagca tctgcacacc ctctgtaatt ggacgtacaa cactcactat
    19141 tatttcaagg ttttggttaa taaagttaga catttcgttt agtttattgg caagaatatc
    19201 aaaaacaccc gaagtatttg aatacttgct cgatacgata gatgcgtt
//
LOCUS       DAOTGZ010000024        19223 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_3064
            NODE_3422_length_19223_cov_4.978610, whole genome shotgun sequence.
ACCESSION   DAOTGZ010000024 DAOTGZ010000000
VERSION     DAOTGZ010000024.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868088
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 19223)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 19223)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:22:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,785
            CDSs (total)                      :: 2,751
            Genes (coding)                    :: 2,732
            CDSs (with protein)               :: 2,732
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 12 of 19
            Pseudo Genes (internal stop)      :: 5 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19223
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3422_length_19223_cov_4.978610"
                     /isolate="AMR_MDS_3064"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..676)
                     /locus_tag="P5123_02995"
     CDS             complement(<1..676)
                     /locus_tag="P5123_02995"
                     /inference="COORDINATES: protein motif:HMM:NF014385.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TolC family protein"
                     /protein_id="HRX15259.1"
                     /translation="MKKKIILFFILYPVVIFSQKSDNAKLTLDNCIQIAMQNHRDLRV
                     IEEDDSISYADYRISTAQRLPSVSLGVTTKEIKDEGLESGELPIPGKDTRYGVVAGLT
                     FTYDIYKPGRSESIRISKKNLEKNAIENNIKKNNIKISVQKSYYNYILAYESNRLMDL
                     SFKRYSRILRKITIESQAGLVGPVQMSDWQIKMSSVELDYEESVQNLESARTDLFLAM
                     GLEVDNK"
     gene            933..1005
                     /locus_tag="P5123_03000"
     tRNA            933..1005
                     /locus_tag="P5123_03000"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:966..968,aa:Thr,seq:tgt)
     gene            1016..1097
                     /locus_tag="P5123_03005"
     tRNA            1016..1097
                     /locus_tag="P5123_03005"
                     /product="tRNA-Tyr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1050..1052,aa:Tyr,seq:gta)
     gene            complement(1133..1330)
                     /locus_tag="P5123_03010"
     CDS             complement(1133..1330)
                     /locus_tag="P5123_03010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016280598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1858 domain-containing protein"
                     /protein_id="HRX15260.1"
                     /translation="MAEITKDMTFGDLLQQKPEAAEILASYGLHCIGCHIAVFETIEQ
                     GAKAHGLSDEQITEMMDKIIA"
     gene            complement(1351..3954)
                     /locus_tag="P5123_03015"
     CDS             complement(1351..3954)
                     /locus_tag="P5123_03015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013968601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016772 - transferase activity,
                     transferring phosphorus-containing groups [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PEP-utilizing enzyme"
                     /protein_id="HRX15261.1"
                     /translation="MNKSVIFFNKSITPVDEEVREKLGIRGRRAVDLANVGLPIVPGF
                     IIDSELTKKLPEVNIKQIIKTNVGLIEKEMKKVYGDPSNPLLLKVVLSSDLNVPYFPS
                     FHNIGLNDDTVKGFASQTSDEKFAYGEYAYMLRGLGTKLCDADGAIFSASKKDSGKFD
                     AQNALKAIDADKKHVDCLLDGDIYDQLISILKRSSEKFCDSDIDVDNSMSIMVQAMVY
                     GNLGENSYSGSYYTRDIINGDSAISGSFLQNEFDIFNKKPKEISKIGKEYYNRLQEIG
                     NKIEEQFKEIRSIKFTIEEGNLWLIEQREVDENSTQSHLLMLLDLQKRDIIDKDFLVK
                     HIQPAQLNELLHPIIDNSSVKTIKKIQGGISGSTGACIGRVFFSTEKLLEEHKRAIMH
                     GKETNFILCMEATYAEDVKAIEVAKGVISTEGGYSSHAPVVARSLGKVAMVQPNMKIR
                     EKSFTIGNVTVNEGAYVSLNVPYYEEPTIFFGKAGLIEPDFEKNGLLELVNILQEYID
                     DFDVRANADQPREAKLSRNFQAQGIGLCRTEHMFFNEKRIMKFREMILAGSESERRKV
                     LKQLQPMQRSDFYELFKIMEGFPVTIRLLDAPLHEFLPRTDDSMKEFIKYMQTKNKSL
                     KASEIQATCADLSEMNPMLGHRGCRVAITYPEIYEMQCRAIFEAVCMLKKEGIEAVPE
                     IMIPVVMTETELKFVKNGKKIEGTTVKGIRDIRNEVLEEYGVDEIKYSVGSMIELPAA
                     AFRAGKIAQYAEFFSFGTNDLTQTTYGLSRDDVNSFFPSYTLYDLVKNNPFQVLGDEV
                     KEIIGVATQRGRLTRPNLKLGLCGEHGADPENIEFCKRIGLNYVSTSPYSIPLAKLAI
                     AQLNLTEREEE"
     gene            complement(4007..4939)
                     /locus_tag="P5123_03020"
     CDS             complement(4007..4939)
                     /locus_tag="P5123_03020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015712230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRX15262.1"
                     /translation="MLNSTYFALGSKNSFNLALRHFYKVNDLIDDIESHQNIADLINV
                     QLDEEEIQMDQVLPVIGSIIRDKYQYTYHSFDIPVSINDFAKITDETSKWTALDVVMV
                     YYNPNGQIYVINPKNEDAWERVRELACDHLVVIYAKYLKGDGDPKIEKEAITAIEEMI
                     AGKDVYINKDFVDASVAPRRAAKPSKPAPQAVAGAPKPTGKRRATPKYSVQVTNELFH
                     NGNVEAWKKIIQSFNAKHPDLEVIIFYEGELINDINALFKWGKVKHHGLIMFQVVGEN
                     IRNVSKLQKYLFEGASQRFEQFLKGQVGQVLNLF"
     gene            complement(5162..7105)
                     /gene="cfpA"
                     /locus_tag="P5123_03025"
     CDS             complement(5162..7105)
                     /gene="cfpA"
                     /locus_tag="P5123_03025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002769742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytoplasmic filament protein CfpA"
                     /protein_id="HRX15263.1"
                     /translation="MSITNFPQSPNKVHPEIPNAVGSRNSLAQEGRDKINEAITILDE
                     TTDKVMNHIEHKLPEEVLVKMDITGGIREKLYNYINQTYVNMFNRYTVTMEDEFIKKV
                     RDFVDREESKGLARYTPREMTELLDKIGGADKFNTGEVEKSMINMYGHLQGHIQRGMN
                     DLENETNAILRQKTDVGAFIRGENAYAILKCVIKDNEMRPKYVNDVKLSLNILDSELI
                     SPIYHYQVTVETLLKDAIQKHVQDLIDRQIDDLKEELVDQGHSEMTGSEIMFEKIKRV
                     ENFTDDEMEDEKSKRYTILAKKFLDKIEGLRAEIDIEDYDPLNIRENIKFVIDEENIR
                     NRGYNTTINAITSILDTSKLGYQVCDNMKNARVCFIKEYEELDKTVLPDERYDIKLCY
                     MDQNQIREACKEFDRQMDAFTREIQLLTDVADAHYQSTKRFRALKDFDDLSKRLMSRE
                     WLRRREAERQDPAYVHPDDIGMLYCENSFVEKNNRTYEDRIKYLKNKLRHLREHLNKI
                     HGYQNPVERVILDEKILFVTKKFNEFTYEVNPHHIQPGLILDLNVTTIKRKQFMLKNM
                     ANVLNEFLYGVSKGFQDAAFAQFKRRRSTIRTDIDQSFVTEEDTDSIFQMAYGAAAEA
                     ASDVAGTADLTPKKGRGSGLKEL"
     gene            complement(7264..8445)
                     /locus_tag="P5123_03030"
     CDS             complement(7264..8445)
                     /locus_tag="P5123_03030"
                     /inference="COORDINATES: protein motif:HMM:NF012321.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HRX15264.1"
                     /translation="MKVKVLSLLLSFLILPVLFFSCSSDDVEPQKELRANQDIILVLD
                     TSLSMIGKAKGARDIFPQVKERTARFIDTVSTGDTFTFITFDTEPRVYPTIKVKNESD
                     LNVIKSFVSQVEAKGQWTFTMEMFRDVLKKADEIRRLNLLEKSKAEEEGVEFNERKQI
                     VIILSDALDDPPPSQKSERLSVKDVASEHTGEDWFVYFVSLGQLTDNEKLQALQDELS
                     SDTSATVGVLNASSDVGDGFSTIEQETKERKSDISGSSRPVYKAAWFYLILIFLVILA
                     IGIIYAIHSARIKLSGMLEYRNKVVLGSKFQKINLERYDSRRINIGKDSLCELRLADF
                     ETHLPVCLEAALVKGELFVFVNTDRGIKVQITEGKGDPYLSDGAVFEAGGYLFRYVTR
                     K"
     gene            complement(8895..9698)
                     /locus_tag="P5123_03035"
     CDS             complement(8895..9698)
                     /locus_tag="P5123_03035"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRX15265.1"
                     /translation="MKYLLDKPSKLNGVAMFQPYFARLEKHLIKIYQTGDKKSVKKTT
                     KSSKTAVKKSTEKSLVKTTKSGGDYEDLIKAVNGMLPEGNIITRSKICDSTGISVDTA
                     DFIVSEKRFKGMELIFSEEMPVELVRGSYSICQKVNRKQLVETLINVANCKKVGQYEK
                     KNEPPFHASFVIAFDSTYQMDELKKAVKEIYHENSVDPRFEMDLMIVLGKGIVIKDWS
                     KKGNYIALDTKEDSLMWFYIIMNEYLSLATSQDFDLRNYIRNPKVYDEF"
     gene            9900..10760
                     /locus_tag="P5123_03040"
     CDS             9900..10760
                     /locus_tag="P5123_03040"
                     /inference="COORDINATES: protein motif:HMM:NF014234.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004665 - prephenate dehydrogenase (NADP+)
                     activity [Evidence IEA];
                     GO_function: GO:0008977 - prephenate dehydrogenase (NAD+)
                     activity [Evidence IEA];
                     GO_process: GO:0006571 - tyrosine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prephenate dehydrogenase/arogenate dehydrogenase
                     family protein"
                     /protein_id="HRX15266.1"
                     /translation="MPNFFKQTKDISVGIIGGTGGMGKLFAHFFERNGIRCEAASRST
                     SLGIEECASRNDIVIITVPIASTLETIRKVAPLVKEDGLLMDLTSLKKREVECMLDNS
                     KCEVIGCHPVFGPSVSSFEKQVIVLTPSRGDKWLNLLKYLFDKENAVVKISTPEEHDR
                     IMSVVQGLMHFTSITLIKTLMKTEMPSEIFNEYSSPVYRIRNDFASRILNQNPELYAD
                     IELFNPETVPILKKYIQSCRELMEVVENNDRDGFINEFNTASGYIGDYKDTAEKKTNQ
                     IIDFVASLKG"
     gene            10764..11480
                     /locus_tag="P5123_03045"
     CDS             10764..11480
                     /locus_tag="P5123_03045"
                     /inference="COORDINATES: protein motif:HMM:NF012304.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004523 - RNA-DNA hybrid ribonuclease
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="reverse transcriptase-like protein"
                     /protein_id="HRX15267.1"
                     /translation="MIKIYTDGSFINGKCGFGAVISNNGTIMEEISGAVTDPQSLRHR
                     QVAGEVEAVLRALKWCSNNGVNECEIYFDYQGVQSWATGGWKRKTALTQTYHDLLVKS
                     EIKIKWVKVKAHSGDPMNDKADRLAKAGCGLANNEVMIKKDPLEITTNVLLDQAEKIG
                     IDFTEYLNSEDIDAYYSGTYNDMYSRVEIGSGKDRIGIVDIYNTKKKSLKPDFRGFKN
                     DFDKKIINDFWDRFLKASLN"
     gene            11622..14837
                     /locus_tag="P5123_03050"
     CDS             11622..14837
                     /locus_tag="P5123_03050"
                     /inference="COORDINATES: protein motif:HMM:NF013069.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HRX15268.1"
                     /translation="MKKIIEYFIANPLIVNVITIFVLLSGFTMYQAIQKEGYPPVEEP
                     GCSISAEYPGASPSDVELNVAMKIEEKIENISGIKSYTSTSYENYCQLTVFFENDEDY
                     AEIRDNIRREIDAITDFPAEMTDAPKIHEWGSKYMDLMQIGIYSKEADYSELKKRYED
                     LKERIEELPHISEVDAWGDRDREIHIKADLDKLNNLYITFNSITEAIRTNNIQISAGS
                     VKEIDSEKSLITLSQFEKPYDINDVILRSNLEGDMIKLSDVAKVEDTFANIDSVIRFN
                     GHNGFAMAIYKKEKADIITSVEAVRKVINDYQKEIGTERVGITILLDNSEQTKSRLSI
                     VKNNALGGLLLVALMLFLFLNFKNALWTVLGIPFSICFGMIFLKASGTTVNSVSLLAM
                     IIVLGMIVDDAIVISENIHRHRILNGTGADSTVKAVLEVGFAVLTTILTTLVAFVPLF
                     FMEGVIGSYVKEVPLVISLLLIGSLLESLFILPSHISHHLTTVQRILLGCTIGAATGY
                     FTSQLAGQSLFIGFIASVIGIALFGTLFAFFYKEQTRIEERHYVVALRNIYGRFLTHV
                     LHFRYIALLGLFVILFAGLYLLQNMKFEMFPSVEANRLTIKGETKDNRSVSYTSEKLS
                     EIEKFIETTYDKKTVSSYVSINGTSSNPEKFTLILFLTPESGRDVKTKSIIDSLRDKF
                     DKDIFENMNFGTSDGGPVIGNNVEIEISGNNDIDRNNIATMIEEDLKMMKGTNEVFRS
                     DSSSKTQVQIKPRYEDMARLGISPVKLATIIRVAFEGHIVTYLKSPEGDIPYRLVLDD
                     NYRNRVSTLDKLMVQASTRNLYNISSMITVAEKEAVTQIKRYNGKRTATIWADFDTHK
                     ITANEIYTLLSEKYRDIPKKYHGIRINLGGAAELSNEALSSLKSTMIVAVTAIFVLLV
                     FLFRSLSQPFIVIVAIPFGMLGVIFAFNIHGMPMSFMGLMGAIGLSGVVVNDSLIMVD
                     YINQLKKSNDKKSLKEIIICGSTTRLRPIILTTATTFAGLLPTAYGIGGKDQFILPTA
                     ISLSWGLFFSTILVLILLPCFYLIEQDISKWTKRAMAIIMQ"
     gene            14985..15422
                     /locus_tag="P5123_03055"
     CDS             14985..15422
                     /locus_tag="P5123_03055"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRX15269.1"
                     /translation="MKIECTKKEYEILAQLVYLGEYMINGILMPEDWDKDITKVKNDI
                     LSLAKDHDFIQNVTKDKDDSYNLTADYESEVMDYVSDYDDECFWQELPIRLAQRDLVA
                     EFGESQLEKMSPNEIHDRETLLLTKYQEELEENGIMNIGIIAK"
     gene            complement(15462..16301)
                     /gene="nadC"
                     /locus_tag="P5123_03060"
     CDS             complement(15462..16301)
                     /gene="nadC"
                     /locus_tag="P5123_03060"
                     /EC_number="2.4.2.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013451430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004514 - nicotinate-nucleotide
                     diphosphorylase (carboxylating) activity [Evidence IEA];
                     GO_process: GO:0019363 - pyridine nucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carboxylating nicotinate-nucleotide
                     diphosphorylase"
                     /protein_id="HRX15270.1"
                     /translation="MGDFAISDIETLIKLAIDEDIRTGDVTGDAVFKKDEVSEAYIVS
                     KDDGIFCGGFIPGYVYGYLDKSINCRTFFEDGSQIKKGDVVMEFNGPTIQLLSGERIV
                     LNFMQRMCGIATETDRVVSQLKGSSIKILDTRKTLPGFRLLDKYSVKTGGGTNHRMGL
                     FDMVMIKDNHIKSAGSIAKAVALVRKRWNNFYKIEVETTTLDEVREAVQSGADIIMLD
                     NMNREMMQEALKIIMKKAEVEISGNMSAEKITQLKDLDVDYISIGALTHSVKAFDLSM
                     KFR"
     gene            complement(16351..17358)
                     /locus_tag="P5123_03065"
     CDS             complement(16351..17358)
                     /locus_tag="P5123_03065"
                     /inference="COORDINATES: protein motif:HMM:NF024491.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospholipase D-like domain-containing protein"
                     /protein_id="HRX15271.1"
                     /translation="MFRFKFIFAFLVLVITFNSYSESRWYSVIFTTPGNIENERSVVE
                     YFCKVIKSSKRNIDAAFFKIDSPDVIEAFIGQAAKGVRVRIVTDDGQTSSKTIDYLKA
                     NGVSVESDGRSSFMHNKFMIIDSTYVWTGSLNVTNNGFSKNNNNSILIKSNDLAKIYS
                     YEFREMYEYGVYQNRKEQKFFKLPDNRYYVKIGDTNINCYFSPDNDIERILLKRILKA
                     KKSVVFMAFSFTSEAIGDALVTVHKKGIKVSGIFEKRGTGSKHSEYSKLNSAGIRVIK
                     DSNPAVMHHKVIIIDGETVITGSYNFSKNAAKRNDENVIIIKNRQIAEEYLKEFIRLY
                     K"
     gene            complement(17351..18298)
                     /locus_tag="P5123_03070"
     CDS             complement(17351..18298)
                     /locus_tag="P5123_03070"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRX15272.1"
                     /translation="MNIMEEKTPKGYILKINKLKMNFLVFDRLGSTVYADSEQIESIH
                     KIPHIVFYGSGFILKRRIVGEYNLLKVSRGKILIPYYVKNNKDLHYHLLPTNEQSDMI
                     PVKVIKIEKPLEFIRYIESGNKPDYVIIEENIDETDQAVLYDRYAQGNFIRIDIERAD
                     LVSRENYENVDEDYHSVNLNMMSGNPVFLSIIHIQNFNLSGVRQLLLDFDLDEEQISY
                     MIAYMTIQINNSSFPSPKKNMEKMLQLRKEMNFYQFLLIHDKENCSELIDEITDSATL
                     NSFLTLLAKVSSMTDDESNLDMVDIQNMLYEKKEALAGV"
     gene            18650..>19223
                     /locus_tag="P5123_03075"
     CDS             18650..>19223
                     /locus_tag="P5123_03075"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRX15273.1"
                     /translation="MPINIARRSVYQAALLSVSSESAPNCATNLVSESGVWKPRKGNS
                     TFDFVILDFSCEVNANFIEISAQENFPQNFRIEISSDNDVWQILQTENGFILNDENYT
                     LHFPLKTFRYIKLFIHYNDSAGEVPVIKNITAGISGITTVEVNDTYQKESSLANLFDG
                     SDSPFSASCTKPERLEMVVDLNTVSPVTAVG"
BASE COUNT         6035 a         3794 c         3187 g         6207 t
ORIGIN      
        1 ttttattgtc tacttcaagc cccatcgcta aaaacagatc ggttctggca ctttcgagat
       61 tctgaaccga ttcttcataa tccagttcga cagatgacat ttttatctgc caatcactca
      121 tctgtaccgg tcctacaagc cctgcctgcg actcaattgt tatttttctt aaaattcgcg
      181 aataacgttt aaatgataaa tccattaaac ggttggattc ataagcgaga atataattat
      241 aataagattt ttgaacggat atttttatat tgttcttttt tatattattt tctatagcat
      301 ttttttcaag attttttttg gaaatcctga tactctcgga tcttccaggt ttataaatat
      361 cataagtaaa agtcaaacct gcaacgacac catatcgagt gtccttacca ggaattggca
      421 attcacctga ctcaaggcct tcatccttaa tctctttagt agtaacgccc agactgaccg
      481 aaggtaatct ttgtgccgtt gatattctgt aatcggcata cgatatggag tcgtcctctt
      541 ctataacacg taaatcacga tgattttgca tagctatctg gatacagtta tccagtgtca
      601 gttttgcatt atcagatttc tgtgaaaata taacaaccgg atacaatata aagaataata
      661 ttattttttt tttcataaat aagtttacca tttattaagc ttgtaccact tctcagtata
      721 ttttttatgg tgtcaaacac atttaccaac aagaaagttt gaaatcagcc aaaatatgga
      781 gtatagttat tgctgatgca ttgtataaaa tatcatgtat gtaatattgt tttgggtaat
      841 tcttttcatc aaggttacta ataatttttt ttgtttatgt ggatttttct tgacacttta
      901 gagctcacat taaatatgtg atatcaatga atgctggtgt agctcaactg gtagagcaat
      961 tgatttgtaa tcaataggtt gggggttcaa gtcctctcac cagctcattg aaaacggtga
     1021 ggttcccgag tggccaaagg gaacagactg taaatctgtc ggcgtcgcct tcgaaggttc
     1081 gaatccttcc ctcaccaata aaaaagccgg ttcttacgaa ccggcttttt tattatgcaa
     1141 ttatcttgtc catcatttca gtgatctgtt catcacttaa gccatgcgcc ttggctcctt
     1201 gctcaattgt ctcaaatact gcaatgtgac aaccgataca atgaagacca taagacgcca
     1261 gaatttcggc agcttcaggt ttctgctgaa gcagatcacc gaaagtcata tctttggtta
     1321 tctcagccat caaatcattc ctccgataaa ttattcttct tctctttccg tcaaattaag
     1381 ctgtgcaatt gcaagttttg ccaaaggaat cgaatatgga gatgtagaaa cataattcaa
     1441 accgattcgc ttacaaaact caatgttttc aggatcagca ccatgttcac cgcataaacc
     1501 caactttaaa ttaggcctgg ttaaacgccc tctctgagta gctactccaa ttatctcttt
     1561 tacctcgtca ccaagcacct gaaaagggtt gttttttaca aggtcataaa gagtgtaact
     1621 tggaaagaaa gagttcacat cgtcacggga taggccatac gtagtttgtg tcaaatcatt
     1681 tgttccaaaa ctaaaaaatt cggcgtactg cgcaattttt cctgctcgaa aggccgccgc
     1741 aggaagctca atcattgaac caacactata ttttatctcg tcgactccat actcctctaa
     1801 aacttcattt cttatgtctc ggatcccctt aactgttgta ccttcaattt tcttaccatt
     1861 ttttacgaat ttcaactcag tttcagtcat tacaactggt atcattattt caggaacagc
     1921 ctctataccc tcttttttaa gcatacagac tgcttcgaaa atagctctgc actgcatttc
     1981 ataaatttcg gggtaagtaa tcgctacacg gcatcctcta tgacctagca ttgggttcat
     2041 ctctgaaaga tcagcgcacg tagcttggat ttctgatgct tttaagctct tgtttttggt
     2101 ctgcatatat ttgataaact ctttcattga gtcatcagta cgaggaagaa attcatgtaa
     2161 aggagcatcc agcaggcgga ttgttacggg gaatccctcc attattttaa acaattcata
     2221 gaaatcagaa cgctgcatgg gctgcaactg tttcaaaacc tttcgccttt ctgattctga
     2281 tcctgctaaa atcatttcac ggaatttcat tattcttttt tcattaaaga acatgtgctc
     2341 ggtacggcaa agcccaatac cctgtgcctg aaaattacga gataacttgg cctcacgcgg
     2401 ttgatcagca tttgctctga catcaaaatc atctatatac tcttgaagaa tattaacaag
     2461 ctcaagtaac ccattctttt caaaatcagg ttcaataaga cccgccttac caaaaaatat
     2521 ggttggttct tcataatagg gaacattcaa cgatacataa gctccctcgt ttacggttac
     2581 atttccaatg gtaaaacttt tttcgcgtat tttcatattt ggttgaacca tggcaacttt
     2641 tcccaaagaa cgggcaacaa ccggcgcgtg agaagagtaa cctccttctg tcgagattac
     2701 tcctttcgca acctcgatag ctttaacatc ttcagcatat gtggcttcca tacataaaat
     2761 aaaattggtc tctttaccat gcattatcgc gcgtttgtgt tcttccaata acttttcggt
     2821 tgaaaaaaac actcttccaa tacatgctcc ggtagatccc gaaataccac cctgtatttt
     2881 ttttattgtt ttaacagaag aattatcaat aatcgggtgc aaaagttcat taagttgcgc
     2941 tggctgtata tgcttaacca gaaagtcttt atcaattatg tcgcgttttt gaagatcaag
     3001 caacattaac agatgagact gggttgaatt ttcatcaacc tcacgttgct caataagcca
     3061 taggttaccc tcttcaatag taaatttaat ggatctaatc tctttaaact gctcttctat
     3121 tttatttcca atttcctgta gacggttgta gtactccttg ccaattttag aaatttcttt
     3181 gggttttttg ttaaagatgt caaattcatt ttgcaaaaat gatccggata tagcactatc
     3241 tccgtttatt atgtcacgag tatagtaact tcctgaatag gaattttcac caaggtttcc
     3301 ataaaccata gcctgtacca ttatagacat agaattatct acatcaatgt cagaatcaca
     3361 gaatttttct gaagaccttt ttaaaataga tataagttga tcatatatat caccgtctaa
     3421 aagacaatct acatgttttt tatctgcatc tattgccttt aacgcatttt gtgcgtcaaa
     3481 ctttccgcta tctttcttgg atgctgaaaa aatagctcca tcggcatcac aaagttttgt
     3541 tcctagacct cttaacatat atgcatattc tccatatgca aacttctcat cacttgtctg
     3601 tgaagcaaac cctttaacag tgtcatcgtt taagccaata ttgtgaaacg aaggaaaata
     3661 tgggacattt agatcagaac ttaaaaccac cttcagcaac agaggatttg aaggatcacc
     3721 ataaactttt ttcatctctt tttcgattag accgacattc gttttgatta tctgtttaat
     3781 gttcacttca ggaagctttt ttgttagctc agagtctata ataaaaccag gcacaatagg
     3841 aagaccaaca tttgccaaat ctactgcacg tcgtccacgt atacccaatt tttctcttac
     3901 ctcttcatcc acaggggtaa tgcttttatt aaagaaaatt acacttttat tcatctactc
     3961 ctccaaaaat aaccgggaac cccggactaa ataacaataa ataattttaa aaaagattta
     4021 agacctgacc aacctgacct ttaagaaact gttcaaaacg ttggcttgca ccctcaaata
     4081 gatacttttg aagttttgaa acatttcgaa tattttcacc aacaacctgg aacatgatta
     4141 atccatggtg tttaacttta ccccacttaa aaagagcatt aatatcatta atcagctcac
     4201 cctcataaaa aataataact tcgaggtcag gatgttttgc attaaaactc tgtatgattt
     4261 ttttccatgc ctcaacatta ccattatgaa aaagctcatt tgtaacctgc acagagtatt
     4321 ttggagtagc tcgacgtttt cctgtaggtt taggtgctcc agcaacagcc tgcggagcag
     4381 gtttgcttgg tttagcagct cttcgtggtg caacagacgc atccacaaaa tccttattga
     4441 tataaacatc ttttccagca atcatctctt caattgctgt aattgcttct ttttcaattt
     4501 ttggatcccc atcacctttc aagtattttg cgtatattac aacaagatga tcacaagcaa
     4561 gttcgcgaac tctttcccaa gcatcctcat tttttggatt aattacataa atctgcccat
     4621 taggattata gtacaccatt actacatcca aagcagtcca ttttgaagtc tcatctgtta
     4681 tcttagcaaa atcatttatt gaaacaggaa tatcaaagga gtgataggta tattggtact
     4741 tatctctgat aattgaacct ataactggta aaacctgatc catttgaatc tcttcttcat
     4801 caagttggac atttattaaa tctgcaatat tttgatgaga ttcaatatca tcaatcaaat
     4861 cattaacttt gtaaaaatgt cgcagcgcaa gattaaagga attctttgag ccaagcgcaa
     4921 aatatgttga atttaacata ataaattctc ccccaaatat ttagatgctg tttaactttt
     4981 tcataataga ttattttttg caagtatttt gttaacagat atataaaatt taaatataag
     5041 cagtattgaa tgccttaact tgtttttttc ttacaattag aagtaaatta catctgtttt
     5101 tgaacgaata aaaaaaggag tgctcacgca ctccttttta ttgtttttaa ttatttgcct
     5161 tttaaagttc cttaagtccg gaacctcttc cttttttagg tgtaagatcc gctgttccag
     5221 caacatcaga agccgcttca gccgcagcgc catatgccat ctgaaaaata ctatctgtat
     5281 cttcttctgt aacaaatgac tgatcaatat ccgtacgaat agttgatcta cgtcttttaa
     5341 actgtgcgaa cgcagcatct tggaatcctt tagatacacc ataaagaaac tcatttagca
     5401 cgtttgccat attcttaagc ataaactgct tacgcttaat tgttgtgaca ttaaggtcaa
     5461 gtatcagacc tggctgtata tgatgagggt taacctcata tgtgaactcg ttaaactttt
     5521 tagtaacaaa aagaattttt tcatcaagaa taactcgctc aacagggttt tgataaccat
     5581 gaatcttgtt taaatgctca cgcaaatgtc taagcttgtt tttaagatac ttaatacgat
     5641 cttcatatgt tctattattc ttttctacaa aagagttttc gcaatagagc ataccgatat
     5701 catcagggtg aacataagcg ggatcctgac gctcagcttc acgtcttcgc aaccattcac
     5761 gactcattag acgttttgaa agatcatcaa aatccttaag agctctaaac cgcttggtag
     5821 attgataatg agcatccgca acatctgtta aaagttgaat ctcgcgggtg aaagcatcca
     5881 tctgacgatc aaattcttta caagcttcac gtatctgatt ctgatccata taacaaagtt
     5941 taatatcata tcgctcatca ggaagaacag tcttgtcaag ttcctcgtac tccttgataa
     6001 agcagacacg ggcatttttc atgttatcac aaacctgata acccaacttg gatgtatcaa
     6061 gaatcgatgt gatagcattg attgttgtat tgtaaccgcg gtttcgtata ttctcttcat
     6121 caataacgaa cttgatattc tcacgaatat taagtgggtc ataatcctca atatctatct
     6181 cagctcgcaa accctcaatc ttatcaagga atttctttgc aaggatagta taacgtttag
     6241 acttttcgtc ttccatttca tcatcagtga agttttcgac acgtttgatc ttctcaaaca
     6301 ttatctctga cccagtcatt tctgaatgcc cctgatcaac aagttcttct ttaagatcat
     6361 caatctgacg atcaatcaag tcctgaacat gtttctgtat agcatctttc aaaagagtct
     6421 ctacagtaac ctgataatga tagatcggag atatcagctc ggaatcaagt atgttcagcg
     6481 acagtttaac gtcattaaca tactttggtc tcatttcatt atctttgata acacatttaa
     6541 gaatcgcata tgcattctca ccacggataa aagctccaac atctgtcttt tggcgaagaa
     6601 tagcgttagt ctcattctca agatcgttca taccgcgctg aatatgtccc tgaaggtgac
     6661 catacatgtt gatcattgac ttctcaactt cacctgtatt aaacttgtcc gcgccaccta
     6721 tcttgtcaag caactcggtc atctctcttg gagtataacg agccagcccc tttgactctt
     6781 cacggtctac gaaatcacga acttttttaa tgaactcatc ttccattgtt acagtgtaac
     6841 ggttaaacat attcacatac gtctggttaa tgtaattata aagcttctca cggataccac
     6901 cggtgatatc catcttaaca agaacttctt ccggaagctt atgttctata tggttcataa
     6961 ccttgtcggt agtttcgtca agtatagtaa tcgcctcatt aatcttgtca cgcccttcct
     7021 gagccaacga gttacgagag ccgaccgcat taggtatctc cgggtgaact ttattaggac
     7081 tctgcggaaa atttgtaatc gacatgtata atcctcctta tttttttcga acaacgatac
     7141 aactataata tcatttaact atttagatac aacgcattcg catagaattt aatttataac
     7201 agctaattgt caagctatta atcaattttg tcctgaaaat tatttgacaa cccgcccatg
     7261 ctcctatttg cgggttacat acctgaacaa gtaacctcca gcctcaaaaa cagctccatc
     7321 actcagataa ggatccccct tgccttcagt gatctgaact ttaattcccc tatcagtatt
     7381 aacaaagaca aacaattctc ctttaacaag agctgcttcc aagcaaaccg gcaaatgagt
     7441 ctcaaaatcc gcgagacgta actcacaaag actatcttta ccaatattta tgcgcctaga
     7501 gtcatatctt tcaagattta ttttttgaaa ttttgatccc aaaacaacct tgtttctgta
     7561 ctcaagcata cctgacagct tgattcttgc cgaatgtatt gcgtatatta ttccaatagc
     7621 tagtataaca aggaagatta atatcaaata gaaccatgct gccttgtaaa ccggccgact
     7681 acttccagat atatccgact ttcgctcctt agtttcctgt tcaatcgtag agaacccatc
     7741 cccaacatct gacgacgcat tcagaacacc gacagttgcc gatgtgtcag acgaaagttc
     7801 atcctgaaga gcctgtaact tctcattatc tgtcaattga ccaaggctta caaagtaaac
     7861 aaaccaatcc tctccggtat gctcacttgc cacatcctta acacttaatc tctcactctt
     7921 ttgcgaaggc ggaggatcat caagcgcgtc actcaatatt ataacaatct gctttctttc
     7981 attgaactca acgccttcct cttcagcctt tgacttttca agcagattca aacgtcttat
     8041 ttcatcagct ttcttaagca catcacggaa catctccatc gtaaaagtcc actgcccctt
     8101 ggcttcaact tgagatacaa aactcttgat aacattcaaa tcgctctcat ttttgacctt
     8161 aatggttggg taaacccgag gttcagtgtc aaatgttata aaagtgaagg tatcaccggt
     8221 agatacagta tcaataaagc gagctgtccg ctccttaact tgcgggaaga tatcccgagc
     8281 acctttggcc tttccaatca tcgaaagaga agtatcaaga accaggataa tatcctgatt
     8341 tgcgcgtaac tctttctgtg gctcgacatc gtcggaagag cacgaaaaaa acagaaccgg
     8401 gagtattaaa aaagataaca ataaagacaa aactttgact ttcataccat gcctcaacat
     8461 ataattaaag ataaacttca aacacaaaaa aactgctatt acaattgcat aacacctcat
     8521 tgaccttgca aagaacacag gaacaatata atatgggaac ttcgaaaaat tacaatttct
     8581 ggaacagcta tggcatttaa agcccatata taaagaaatt tttcgcaagt tccttgttaa
     8641 aaagttaatt attagagatt accataactt ataatcagag caaactaata tactccgaca
     8701 aaattcaata ataacactaa ataataaacg cgaacataga tatgtcaata actatttaaa
     8761 ccctcactat aaagcgctgt ctcataacac aaaaaatttc taaactgatc attatgcgac
     8821 atattatcga ataatcactt aaataaaaat tggcaaagcc ggcacggtcg gaatttttaa
     8881 gaaactcttt gatgttaaaa ttcatcgtac acttttggat tacgaatata attacgcaaa
     8941 tcgaaatctt gactggtcgc aagggacaaa tactcattca tgattatgta gaaccacata
     9001 agactatctt cttttgtatc aagcgcaatg tagtttcctt ttttactcca gtcctttatc
     9061 acaatacctt taccaagaac aatcatgaga tccatttcga aacggggatc aacgctattt
     9121 tcgtgatata tttccttaac cgctttcttc agttcgtcca tctgatatgt agagtcgaag
     9181 gcgataacga atgaagcgtg aaaaggcggc tcgttctttt tttcgtactg ccctactttt
     9241 ttgcaattag cgacattaat taatgtttca actaactgtt ttctgttaac tttttgacaa
     9301 atgctatacg agccacgaac aagttcaaca ggcatttctt cagaaaaaat aagctccata
     9361 cctttaaaac gtttctcaga aactataaaa tcggccgtat ctacagatat acctgtagaa
     9421 tcacatatct tgctacgtgt tatgatgtta ccctcgggca gcattccgtt aaccgctttg
     9481 ataagatcct cataatctcc accactcttg gtggttttca caagactctt ttcagtactt
     9541 ttttttactg ctgttttaga agacttagtg gtcttcttta cacttttctt atcacctgtt
     9601 tggtatattt ttattagatg cttttcaagt cgggcaaaat agggctgaaa catggcaact
     9661 ccgtttagtt ttgaaggttt atccagcaaa tatttcatta ccggtacatt tttcgggatc
     9721 accgatccct ttatgttact atcggtataa aaaaggcatt aataaatcaa ttatttttct
     9781 tgaaagaaga gctttataaa agtcttatga tctaaaggtt gcatcagaaa aataatccgt
     9841 ttggacggat tatcaatgca cctgttttta cttcatatat aatagaccag gataagaaaa
     9901 tgccaaattt ttttaaacag acgaaagata taagtgtagg aataataggc ggtaccggcg
     9961 gtatgggcaa actttttgcc catttttttg aacgcaacgg gataagatgt gaagccgctt
    10021 caagatcaac cagccttgga attgaagagt gcgcctcaag aaatgacatt gtaattatta
    10081 ctgtgccgat agcttcgacc ctagaaacaa taagaaaagt agcccccttg gtaaaagagg
    10141 atggcctgct tatggatctt acctcattaa agaaaagaga ggtagaatgc atgctcgata
    10201 attcaaaatg tgaggttatc ggttgccatc cagttttcgg accttcggtt tctagttttg
    10261 aaaaacaggt tatcgtatta acaccttctc gcggtgataa gtggctaaat ttgctaaaat
    10321 atctttttga caaagaaaat gccgttgtca agatatcaac tccagaagag cacgacagaa
    10381 tcatgtcggt tgttcagggg cttatgcatt ttacctcaat tacattgata aaaacactaa
    10441 tgaaaactga aatgccatct gaaattttta acgaatactc ctctcccgtt taccggatac
    10501 gtaatgattt tgccagtcga atacttaatc aaaatcccga actttatgct gatattgaac
    10561 tttttaaccc tgaaacagtt ccgattttaa aaaaatatat tcagtcatgc cgtgagctta
    10621 tggaggtagt agaaaataat gatcgtgatg gctttataaa tgaatttaat accgcgtcag
    10681 gctatattgg cgactacaaa gataccgcag aaaagaaaac caatcagata atcgattttg
    10741 ttgcttcatt aaaaggttaa tttatgatca agatatacac cgatggttcg tttattaacg
    10801 ggaaatgtgg ttttggagct gtgatctcga ataacggcac gattatggaa gaaatttccg
    10861 gtgctgttac agaccctcaa agtctacgcc atcgccaggt ggcaggtgaa gttgaagccg
    10921 tgttgcgagc actcaaatgg tgttcgaata atggcgtaaa tgagtgtgaa atctattttg
    10981 attatcaggg tgttcaaagt tgggcaaccg gagggtggaa acgcaaaacc gctctgactc
    11041 agacttatca cgacctgctt gttaagtcgg agataaaaat caaatgggtt aaggtaaaag
    11101 cccactcggg tgacccgatg aatgataagg ctgaccggct tgcaaaagca ggatgcgggt
    11161 tagcaaataa cgaagttatg ataaaaaaag atcctttaga aatcaccacc aatgttttac
    11221 ttgatcaggc cgaaaaaata ggcatcgatt ttaccgaata ccttaatagc gaagatattg
    11281 atgcctatta ttcaggtaca tataatgata tgtattcaag agtcgaaatc ggatcaggaa
    11341 aagatcgcat aggtattgta gacatataca acacaaagaa aaaaagtctt aaacctgact
    11401 ttcgtggctt caaaaacgat tttgataaaa aaattatcaa tgatttttgg gatagattct
    11461 tgaaggcttc tcttaactga atattcttca atattgtata gatatactgt ctctgataat
    11521 actattttac catttttttt acaccaatag ggcgctatca ctagttattt tgttttatta
    11581 aatacaattc taaatcttct atttcccatt taggttttca gatgaaaaaa attatagaat
    11641 acttcatcgc caatcctctt attgtaaacg taataacgat atttgtactg ctatccggtt
    11701 ttacgatgta tcaggctatt cagaaagagg gctatccgcc tgtagaagaa ccggggtgtt
    11761 caatcagcgc ggaatatccc ggagcatcac cttctgatgt cgaactaaat gttgcaatga
    11821 aaattgaaga aaagatcgaa aacatttccg ggataaaaag ctatacctca acatcttacg
    11881 aaaactattg ccagttaact gttttttttg aaaacgatga agactatgcc gaaatacgcg
    11941 acaatataag acgcgaaata gacgccatta cagatttccc cgccgagatg accgacgcac
    12001 ccaagataca tgagtggggc tccaaatata tggatctgat gcaaatcggc atctattcaa
    12061 aagaggccga ttactctgaa ttgaaaaaga gatatgagga tttaaaagaa aggattgaag
    12121 agttacctca tatttctgaa gttgatgctt ggggtgacag ggatcgtgaa attcatatta
    12181 aagcagatct tgataaacta aataatctgt atataacttt taattctata accgaggcaa
    12241 ttcgaactaa caatattcag atatctgccg ggtcagttaa agaaattgat tcggaaaaga
    12301 gtttaattac actctcacaa tttgaaaaac catatgacat taatgatgtt attcttcgta
    12361 gtaatttaga aggggacatg attaaacttt cagatgttgc taaagttgag gatacatttg
    12421 ctaatataga ctctgttata cgttttaatg gtcataatgg atttgcaatg gcaatttaca
    12481 aaaaagaaaa agctgacata attacctctg ttgaagcagt tcgaaaagtc atcaatgatt
    12541 atcaaaaaga aattggaacc gaacgtgttg gaataactat acttctggat aattccgaac
    12601 agacaaaatc acgtttaagc atagtaaaaa acaatgctct tggtggatta ttgcttgttg
    12661 ctcttatgct ctttcttttt ctgaatttca aaaacgcatt atggactgtc ttgggaattc
    12721 ctttttccat atgttttgga atgatctttt taaaggcaag tggtactacg gtcaactcgg
    12781 tttctctact tgctatgatc atcgttcttg gaatgattgt agatgacgca atagtaattt
    12841 cggaaaatat tcacagacat agaatactta acgggacagg agccgacagt actgttaaag
    12901 ctgtacttga agttggattt gcagttttaa caactattct aacaaccctt gtagcttttg
    12961 ttcctctttt ttttatggaa ggagtcatag gatcatacgt aaaagaggtt ccacttgtaa
    13021 tttctcttct tcttataggt tcgcttcttg aatctctttt tattcttcca tcacatatct
    13081 ctcatcatct caccactgta cagcgtatac ttcttggttg cactattggt gcagcaacag
    13141 gatattttac atcacaatta gcaggacaat cgttatttat cggatttata gcctctgtaa
    13201 tcggtattgc actatttggt acactatttg ctttctttta caaggaacag accagaattg
    13261 aagagcggca ttatgttgtt gctcttagga atatttacgg ccgatttttg actcatgttc
    13321 ttcattttag atatattgca cttttagggc tctttgttat attgtttgcc ggtctttatc
    13381 ttttacaaaa tatgaaattc gaaatgtttc catctgtcga agcgaacaga ttaacaatta
    13441 agggggaaac aaaagataat cgttctgtca gttatacctc tgaaaaatta tccgaaatag
    13501 aaaagtttat cgaaacaacc tatgataaaa aaacagtgtc gtcatatgtt tccataaacg
    13561 ggacatccag caatcctgag aaattcactc tgatactctt tttaacaccc gaaagtggaa
    13621 gagatgtaaa aaccaaatca attatagatt ctttacgcga taaattcgat aaggacatat
    13681 tcgaaaacat gaattttgga acatctgacg ggggtccagt aatcggaaac aatgttgaaa
    13741 tagaaatcag cggtaataac gacattgaca gaaacaatat tgctactatg attgaagaag
    13801 acctaaaaat gatgaaggga acaaacgagg ttttccggag tgacagtagt tctaaaactc
    13861 aggtacagat aaagcctcga tacgaagata tggcacggct tggaataagc cctgttaagc
    13921 tggctacgat aatccgtgtt gcctttgaag gtcacatagt aacatatctc aaatctccag
    13981 agggagatat cccatatcga ttggttcttg atgataatta ccgtaacaga gtatcgaccc
    14041 ttgataaact aatggttcag gccagcactc gtaacttgta caatatatca tctatgatta
    14101 cagttgctga gaaggaagcc gtaactcaaa ttaaaagata taatggcaaa cgcacagcaa
    14161 ccatctgggc cgattttgac acacataaga ttactgctaa cgaaatttac actttgcttt
    14221 ctgaaaaata cagggatatc ccaaagaaat accatggcat aagaatcaac ttaggaggcg
    14281 cggctgaatt aagcaacgaa gctctaagct cgcttaagag cacaatgatt gtagcagtaa
    14341 cagcgatttt tgttcttctt gtgtttcttt ttcgttcttt atcgcaaccg tttattgtaa
    14401 ttgttgctat tccgtttgga atgcttggtg ttattttcgc ttttaatata catggaatgc
    14461 ccatgtcttt catgggttta atgggagcga taggtttatc cggagttgtt gtaaatgatt
    14521 ctctgattat ggttgattac attaatcagt taaaaaaaag taatgacaaa aagtctctta
    14581 aagaaattat tatttgcgga tcgacaacca gacttagacc gataatctta acaactgcaa
    14641 caacttttgc aggactattg cctacagcct acggtatagg aggtaaagat caatttattt
    14701 taccaactgc catatcgcta tcctgggggc tgtttttttc aacgattctt gttcttatac
    14761 ttttaccatg tttttatctt attgagcaag atatttcaaa atggactaaa agagcaatgg
    14821 ctattatcat gcaatgattc tgtgaactat atcagaagtc ataatcagag accagcaatt
    14881 tattggcttt ttgtgacaat ttttctcgca agctcaaaaa agtgaactaa tagagtttgc
    14941 catatatttg tccataaaaa ttaaacacat taacggagat aggcatgaag atagaatgca
    15001 caaaaaaaga gtacgaaatt ttagcacaac ttgtttatct gggagaatat atgattaacg
    15061 gtatattgat gccagaggat tgggacaagg atatcaccaa agttaaaaat gatatattgt
    15121 ctcttgcgaa agaccacgat tttattcaaa atgttactaa agataaagac gatagttata
    15181 atctgacagc ggattatgag tctgaagtaa tggattatgt ttcggattat gatgatgaat
    15241 gtttctggca ggaattaccc atcagactgg cacagcgtga tcttgttgcc gaatttggag
    15301 aaagtcagct tgaaaagatg tcacctaatg aaattcacga cagagaaacc ctgcttctta
    15361 caaagtacca ggaagaattg gaagaaaacg gaataatgaa tatcggaatt attgcaaaat
    15421 aatattcagc cgcctatggc ggctgataaa caaagtattg cttatcgaaa tttcatcgaa
    15481 aggtcaaaag ccttaacgga atgggtcagt gccccaattg atatatagtc aacatcaaga
    15541 tctttcagtt gcgtaatttt ttcagcactc atatttccgc ttatttcgac ttccgctttt
    15601 ttcatgatga tttttaaagc ttcttgcatc atttcgcgat tcatgttatc aagcattatt
    15661 atatcagcac cactttgtac cgcttccctc acctcgtcta gagtagttgt ttcgacctca
    15721 atcttataaa agttattcca gcgttttctc acaagagcaa cagcttttgc tattgaccct
    15781 gcagatttga tatggttatc cttgatcatt accatatcaa atagccccat tctatgatta
    15841 gtacctccac ccgtttttac agaatacttg tcaagcaaac gaaaaccggg aagagtctta
    15901 cgagtatcaa gtatcttgat actgcttcct ttaagttgag atactaccct atctgtttca
    15961 gtggcaattc cacacattct ttgcataaaa tttaaaacaa ttctttcacc agaaagcagt
    16021 tgtatagtcg gaccattaaa ctccataaca acatcccctt tctttatctg actcccatct
    16081 tcaaaaaaag ttcgacagtt tattgatttg tccagatagc cataaacata accggggata
    16141 aatcctccgc aaaatattcc gtcatcttta gatactatat aggcctcaga tacctcatcc
    16201 tttttaaata cagcatcgcc agtaacatcg cctgttcgaa tatcctcatc tatcgcgagt
    16261 ttaattaaag tctcgatatc actaattgca aaatcaccca ttttattctc cgatttagta
    16321 aagagtacac taattgcttg taaaacataa ctatttatat agtctgataa attcttttag
    16381 atactcttca gctatttgtc tgttttttat aatgataaca ttttcatcat tgcgtttggc
    16441 agcatttttt gaaaagttgt acgagccagt aataaccgtt tcaccatcaa taattattac
    16501 cttgtggtgc ataactgcag gattagaatc ctttataaca cgtatgccgg ctgaatttaa
    16561 ttttgaatac tctgagtgct tgcttcctgt acctcttttt tcaaaaatac cggaaacttt
    16621 tatccctttt ttgtgcacag tgacaagagc gtcaccaatg gcttcgcttg taaacgaaaa
    16681 agccataaaa accactgatt tttttgcttt aagtatgcgt ttcaataaaa tacgctcaat
    16741 atcgttatcc ggagagaaat aacagtttat attagtatcc cctattttca cataatatcg
    16801 attatcagga agtttaaaaa atttttgttc tttgcggttt tgataaactc cgtattcata
    16861 catttctctg aattcataac tataaatttt cgcaagatcg ttagatttaa tcaatatgga
    16921 gttattatta tttttagaaa agccattatt tgtgacattt aacgatcctg tccagacata
    16981 ggtcgaatca ataatcataa acttgttatg cataaacgaa cttcttccat cggactcgac
    17041 agatacacca tttgctttga gataatctat tgttttagaa gacgtctgtc cgtcatcagt
    17101 aacaattcga accctgactc ctttggcagc ttgaccaata aatgcctcta ttacatcggg
    17161 cgaatctatt ttaaaaaaag cggcatctat attccgtttt gaacttttga ttactttgca
    17221 aaaatattct acgactgagc gctcattttc aatattacct ggagtcgtga atattacaga
    17281 gtaccaacga ctttcagaat agctgttaaa ggttatgaca agcacaagaa aagcaaatat
    17341 aaacttaaat ctaaacacca gcaagtgcct cctttttctc ataaagcata ttctggatat
    17401 cgaccatatc aagattactt tcatcatcgg tcattgaaga aacttttgcc aacagtgtta
    17461 gaaaagagtt taacgtagcg ctatctgtta tctcatcaat taattcagaa caattctcct
    17521 tatcatggat tagtagaaat tgatagaagt tcatctcttt tcgaagctga agcatctttt
    17581 ccatattttt ttttggtgag ggaaaagaac tgttattaat ttgtattgtc atatatgcga
    17641 tcatgtacga aatctgttct tcatccagat caaaatcgag aagaagctgc cttactccgg
    17701 ataaattgaa attttggata tgtattatgg ataagaaaac cggatttcca gacatcatat
    17761 taagatttac actatggtaa tcttcatcaa cattttcgta attttctctt gatacaagat
    17821 cagccctttc aatatcgatc cttataaaat taccctgcgc gtaacgatca taaagtactg
    17881 cttgatcggt ttcatctata ttctcttcta ttattacata atccggttta tttccagatt
    17941 caatatatct tataaattcc agcggcttct caatttttat taccttgacc gggatcatgt
    18001 ctgactgctc gttagtaggt aaaagatgat aatgaagatc tttgttgttt tttacataat
    18061 aaggaataag tatcttccct cgggaaactt ttaagagatt atactcgcct acaatacggc
    18121 gtttaagaat aaatcctgaa ccataaaaaa caatatgagg aatcttatgt atgctctcta
    18181 tctgttcgga atcggcgtat acggtactcc caagtctgtc aaaaaccaga aaattcattt
    18241 tcaacttgtt tatcttcaaa atgtaccctt ttggggtttt ttcttccatt atattcacga
    18301 ttcggtctcc aaaaccacat tatctggcat tcatttcata aggaaaatca ctttttttga
    18361 tattccttta ttagaaccag ctactaaata attattggca atctctgata attaactttt
    18421 taacaaaaaa cttgcgaaaa aatcctttat atatgggctt taaacggcat agccgttcca
    18481 gaaatggtaa tttttcgaag ttcacaattg tcactctaag gtctaaataa aacatttgac
    18541 aattgttttt tttatcataa ttagacattg gatgtttatt agcctgcaac ccgtttatga
    18601 ctagcagcca taaaacgccc gactatatat caatatcagg agtatcctaa tgcctattaa
    18661 tattgcccgc agatcagtct atcaggcggc tcttttatct gtatcttccg aaagtgctcc
    18721 caattgcgct acaaacctag tttcagaaag cggagtttgg aaacctcgga aaggtaacag
    18781 cactttcgat tttgtgatac ttgatttttc atgcgaggtt aatgcaaatt ttatcgaaat
    18841 atcggctcaa gaaaattttc cccaaaattt tcgcatcgaa ataagttctg acaatgatgt
    18901 ctggcaaatt ttgcaaacgg aaaacggctt tattcttaac gatgaaaact ataccttgca
    18961 ttttcctcta aagactttta gatatatcaa gctttttatt cattataacg actcggcagg
    19021 agaagttcct gttataaaaa atataacagc cggaattagt ggtatcacaa cagtcgaagt
    19081 aaatgatact taccaaaaag aaagctcgct ggccaatctt tttgatggat cagatagccc
    19141 ctttagtgca agttgcacca aacctgaacg acttgagatg gtagttgacc ttaatacagt
    19201 ttctccggtt accgcagtag gtc
//
LOCUS       DAOTGZ010000025        18952 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_3064
            NODE_3500_length_18952_cov_5.972218, whole genome shotgun sequence.
ACCESSION   DAOTGZ010000025 DAOTGZ010000000
VERSION     DAOTGZ010000025.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868088
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 18952)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 18952)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:22:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,785
            CDSs (total)                      :: 2,751
            Genes (coding)                    :: 2,732
            CDSs (with protein)               :: 2,732
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 12 of 19
            Pseudo Genes (internal stop)      :: 5 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18952
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3500_length_18952_cov_5.972218"
                     /isolate="AMR_MDS_3064"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(141..1970)
                     /locus_tag="P5123_03080"
     CDS             complement(141..1970)
                     /locus_tag="P5123_03080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020613154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histone-lysine N-methyltransferase"
                     /protein_id="HRX15274.1"
                     /translation="MLKSISSYSLVEPDEPALYREQFPFTKIPRILFDSSTVFPSPPK
                     ELWITDTTFRDGQQSRPPYTPGQIADIYTLLHKLGGQKGLIRQSEFFIYSDRDRQAVE
                     LCLEKGYQFPEVTSWIRADKKDFALVKSFNLKETGILTSVSDYHIFLKLKRTREKTMN
                     DYLEIVQTSIENGIVPRCHFEDITRADIYGFCLPFAEKLLRIAEEAKMPVKIRLCDTM
                     GYGVTFPGAVLPRSVPKMVDAFHSKLGYPAEWLEWHGHNDFHKVHVNAATAWLYGCAS
                     VNASLLGYGERTGNPPLEAAVIEYMSLTGDDLIDTRVITEIARYFEQTVKAEIPANYP
                     FVGADFNTTRAGIHADGILKNQEIYNIFDTDALLNRPIRVMVTDKSGLAGIAQWLNEN
                     IPAIVAGEREQVSKRHPGIKHINNWVMQQYEQGRTTSISPDELLIQAKHFIPSLFESE
                     FDSARNEAIAIGKKIAEKIATAPEVESLEVSKLEDFLEKIVKKEGSIQMLAVTKTDGY
                     RLTQVHTQHGEKALFRNLMNKDFKNHVWFCEVLETGEAYYSDLFFSKYTKRLIITAAV
                     PIKDKNGNVKAVMDVDFIFNELVKLITDIPDEIIDERSDKKTD"
     gene            2321..2521
                     /locus_tag="P5123_03085"
     CDS             2321..2521
                     /locus_tag="P5123_03085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002477819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0001072 - transcription antitermination
                     factor activity, RNA binding [Evidence IEA];
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HRX15275.1"
                     /translation="MEQGTVKWFNESKGFGFIQKDNGNDIFVHHTGINAEGFKTLEEG
                     ARVEFRVENSEKGERATSVTQL"
     gene            2684..3145
                     /locus_tag="P5123_03090"
     CDS             2684..3145
                     /locus_tag="P5123_03090"
                     /inference="COORDINATES: protein motif:HMM:TIGR01909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C-GCAxxG-C-C family protein"
                     /protein_id="HRX15276.1"
                     /translation="MNTTIERKADASERVQSALEHYDQGYSCSQSIVLAFSDLFDLDP
                     KHATLISSGFGGGMGRLRRTCGALTGAYMILGLKFGNHSPDDNDSKLDGYRVVRKLTE
                     QFEAIHSTSVCKELLANHSKNQNDPAKRHHKHICRKAIEDAARILESLITE"
     gene            3203..3889
                     /gene="ispD"
                     /locus_tag="P5123_03095"
     CDS             3203..3889
                     /gene="ispD"
                     /locus_tag="P5123_03095"
                     /EC_number="2.7.7.60"
                     /inference="COORDINATES: protein motif:HMM:NF013308.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050518 - 2-C-methyl-D-erythritol
                     4-phosphate cytidylyltransferase activity [Evidence IEA];
                     GO_process: GO:0019288 - isopentenyl diphosphate
                     biosynthetic process, methylerythritol 4-phosphate pathway
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-C-methyl-D-erythritol 4-phosphate
                     cytidylyltransferase"
                     /protein_id="HRX15277.1"
                     /translation="MKNRNHALILAGGSGHRTGCDIPKQMISINDIPLIIHTLRKFEN
                     CNEIDTITVVSHNSYVEDIYKLCLKHDISKIIKCVMGGSTRQESSFLGIRALDARCDD
                     IVIIHDAARPYLNESIIKSAINEAREHHAASAVISVTDTVYIAQNGSIVSVPDRKTVY
                     NVQTPQSFSYQTIVEAHKKALDDKISDATDDIILALNTGIEPVLFEGDVTNKKITTKE
                     DLEEAQRLLF"
     gene            complement(3886..4296)
                     /locus_tag="P5123_03100"
     CDS             complement(3886..4296)
                     /locus_tag="P5123_03100"
                     /inference="COORDINATES: protein motif:HMM:NF018894.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0035438 - cyclic-di-GMP binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PilZ domain-containing protein"
                     /protein_id="HRX15278.1"
                     /translation="MSRAFDKRDTDRISTEAFFVQYKLANDDNVFTVEVLNVSAGGMC
                     FLRDSVLYKKDEIRILFPFKSRKMLLKGVVVRVEGREVAIQFQDKQERIDTFVKIFNN
                     EYREISEENKQHSKSKQSLFYNLNEFKNKDDLPL"
     gene            complement(4298..4756)
                     /locus_tag="P5123_03105"
     CDS             complement(4298..4756)
                     /locus_tag="P5123_03105"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF025054.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HRX15279.1"
                     /translation="MVNIDKIKESDIGDILNLLAPYVKKGIVLERSREDIMAELGAFF
                     IARDNEKAAGVVSYHDYDNNLFEIRSLVVAEEYARSGIGSALVNHVVTLLRERQPQSR
                     IFALTYVPEFFSKLNFELIDKNYFPEKIWKDCVNCPRVDCCNEIALINRG"
     gene            complement(4767..5405)
                     /locus_tag="P5123_03110"
     CDS             complement(4767..5405)
                     /locus_tag="P5123_03110"
                     /inference="COORDINATES: protein motif:HMM:NF016895.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SPOR domain-containing protein"
                     /protein_id="HRX15280.1"
                     /translation="MQNIDFYSGNAQQPNIPDFISRDPEYFSRMARSYEKEGIRKKNR
                     ATRFLVLVLSLCIISFTSGLVVGIKFSGGSEKEIVDPVTRQAMSDIGDKVTDIVGNNE
                     SEGSTEDLPAAEAESLAGKNSDTKSAITSFPKEEYPFVIRIGNKFSANQSQEIADYLS
                     GKGHTVILARQEGFFRIYAGPYKNRREAELSLNRINYYRDNKLFHSALILKR"
     gene            complement(5561..6292)
                     /locus_tag="P5123_03115"
     CDS             complement(5561..6292)
                     /locus_tag="P5123_03115"
                     /EC_number="5.4.99.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR00093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pseudouridine synthase"
                     /protein_id="HRX15281.1"
                     /translation="MAEKMRLNRFLACCGLGSRRKVEKFIEAGDVRVNGESVTLACKV
                     DPENDLVTYKGEPVVQSGVLYYVMLNKPKGYITSVGDPLGRPTIVDLIHDKYWKAGVF
                     PVGRLDKDTEGLLLLTNDGFLAQALMHPSSECKKKYIVSLDRSLEEEDKAKIEKGLRL
                     REFKTGPCSIEYISEQKHSVYMTISEGKKRQIRVSFKKFDYKVKRLIRISLGPLVLGN
                     LHRGMFRDLRPIEIKKLKKYFDKDK"
     gene            complement(6296..7000)
                     /locus_tag="P5123_03120"
     CDS             complement(6296..7000)
                     /locus_tag="P5123_03120"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0070064 - proline-rich region binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SH3 domain-containing protein"
                     /protein_id="HRX15282.1"
                     /translation="MKRSMFLWLIILLTACAVDKSDSTIDEDIETKEVFAVALNDYSA
                     VRFQPQIHSSRTDYLMKGDTVKIIMKSAEESRIARIKDYWYYVQLSSGIVGWTFGSNI
                     RILKEGDGFSIEDYQKQVAQENIERIVKELTGKWWSVTSSGNFTSHSLILYEDKKYRS
                     SRGGVVKKGEYSLDMDKNLIIFSDGATFGNDLTLVQRGVEILIEKETEDYKYRFKKIS
                     SETDDEEKPESYDAEI"
     gene            7450..9354
                     /locus_tag="P5123_03125"
     CDS             7450..9354
                     /locus_tag="P5123_03125"
                     /inference="COORDINATES: protein motif:HMM:NF012437.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009190 - cyclic nucleotide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0035556 - intracellular signal transduction
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate/guanylate cyclase domain-containing
                     protein"
                     /protein_id="HRX15283.1"
                     /translation="MAKKYNISIGFKLITLMSLLITLSLGGIIYFATYYFRDDNEKRA
                     EEVTLDRAELIALQIRTDFKSIIGNSTLLLEKSLLLKTKTEISDTDLLYIAQLTDENS
                     PAITQQLLNDTALLTMDATMVSPDSVVSAEIDAIKGGFNTETKVFNGSLYFKRPVIGI
                     ITPHITEEPGRASSILLIFISMNRFLEAVKTQTIYQSFIVNGTGELIAHHDPELVTAK
                     ANFMNMPLVSTMIESTIDFGKKAFKDEEGNSFIGSFKKTGFSDIGVVTYVDESTAFAA
                     VKNLQERNILLTIIVINIAIIFLFIFSRTLTKPVKALSAAANKIKNGEFDIQLKPTTR
                     DEIGYLTESFGEMALGLSEREKLKNALGKFANPEIAEQALKGEIQLGGERKHAAIFFS
                     DIRNFTSISEKLEPEEVVEFLNEYMSLMVNCVNKTHGMVDKFIGDAIMALWGVPFSHG
                     NDLQNSIDCALLMRKELIKFNKNRGGDKKPIIKIGCGINVGPMVAGQIGSDEKMEYTV
                     IGDTVNLASRIESLNKPFGTDILISEDAYTVVKDRYLTVPMRQILVKGKEKAQQIYAV
                     LSHKDDPNGIKSIEELRNVLDITEVQISDSDFPIENEQKFEILNNNHKDSKSHEKLFN
                     KKDVLYEILD"
     gene            9338..11419
                     /locus_tag="P5123_03130"
     CDS             9338..11419
                     /locus_tag="P5123_03130"
                     /inference="COORDINATES: protein motif:HMM:NF016648.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FecR domain-containing protein"
                     /protein_id="HRX15284.1"
                     /translation="MKSLIKEYTLPFFNLIAIIIAAFLLYLNYTYTLTHSSTVQIGTI
                     IFKQKIAERRYSQQVIWEGITNNSPVYNFDAIRTDKGALAVIELDNGTSIQLEEETMI
                     IIQQSDLGLDIDFENGSINAQKSDDDSSLNIRSGDTVVAVNDGSLNVNKNAGTRDLNL
                     NVSSGTAALNSGGKISQIDSTKIVNISSGEAKIGDKQIILQSPAPAKTFITANPLDIN
                     FNWTSSKESTSNLQISKDKNFSNIMYTVPSATGKATARLENGTYYWRVTLNGTNSETR
                     RISVIRETTPVILRPLNGQSFNYTKKPPVIIFKWNGADTASQYTVEIARMESMNTLHK
                     SINSLESFISVSDLQPGKYYCRLKARYALADRKHELLSPVISFTVNQKSQLDAPKLFS
                     PVNTLINANKKPTVFNWQPVGGADKYKIAISGSSGPLFSAITTSNYFAYNTLLNPGDY
                     TWSVTAMDTEGAESQLNNSAKFTVSSTGKITTISPSSSSRFKTGEAVNLTWRDENNGN
                     NYLVIISKNSLFATTYRERKVSSRFTTFSDLESGNYYWKVYLLNDDDRRLIASNADSF
                     SIYGNVVRPIPVSPINTKFPHDTSSISLRWKPVPDATHYEIEIYQESSLISKLLKRAT
                     TASTSYSLNIASYAPGSYSWKVRSIQKSGSVIVTSSDFSKEVFTKESLKEPEKKEYKI
                     EILNPSKIYIE"
     gene            11458..12306
                     /locus_tag="P5123_03135"
     CDS             11458..12306
                     /locus_tag="P5123_03135"
                     /inference="COORDINATES: protein motif:HMM:NF024895.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane beta-barrel protein"
                     /protein_id="HRX15285.1"
                     /translation="MNCYRRHIILRLIKLPRQVLFMLLLGFIYLSNNSILLSKPKIEK
                     TPRIEWRAISAASRYRVQITKSGSTIIDTKTVSNFISAPLHHGVYKFKITAYNQKGET
                     IGESGWEPLIIQSWSLVSKQYYYVGVGVGFEYITPSWNKINENGNSWNVYGGYDLPIQ
                     SIQLEAILDYQSFSPIYKEDKVSTEIDMYSLNCGVFYFSRLSDLFTAGVRLSPGLSYT
                     TITIEDTEETKEYNSIGLSVILGIGLRFNMSYGFIEHGVEYKQTFLNGEDFMSVRPYL
                     RGGARF"
     gene            complement(12471..15104)
                     /gene="alaS"
                     /locus_tag="P5123_03140"
     CDS             complement(12471..15104)
                     /gene="alaS"
                     /locus_tag="P5123_03140"
                     /EC_number="6.1.1.7"
                     /inference="COORDINATES: protein motif:HMM:TIGR00344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004813 - alanine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006419 - alanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alanine--tRNA ligase"
                     /protein_id="HRX15286.1"
                     /translation="MLVKELRASFIEYFVSKGHRQIHSSPLIPKDDPTLLFTTAGMVQ
                     FKPMFAGIVKTDYKRAASVQKCLRTSDLENVGRTKRHCTFFEMLGNFSFGDYFKKEAI
                     EMAWEYSVKVLKLDPEKIWVSVYLDDDEAFDIWNKIVGIPAERIVRLGKEDNFWGPAG
                     DSGACGPCSELYIDKGLENGCGDKDCKPGCDCERFMEYWNLVFNQFHQDTDGNQTPLP
                     QTGIDTGMGLERLATITQNVSSIYETDEFTRMIEFVCDNLGVEYKGKKAVAVNVIVEH
                     ARALTFAMADGAYPSNEGRGYVLRRILRRALRFARTLGVDEPFIYKMVDVIVEIFGEF
                     YPEIVSASMNIKKVLEGEEKRFLETLENGILRIEQLIVDAKKTGSILSGEDAFQLYDT
                     FGFPVEMTTEIALEEGVTVDMDAFSKEMEKQRQRGKDSWKSADGSYEKLVSEICNGIE
                     ESVFTGYSDYSTESKICVLASGSELKEVLSEGMEGYIVLDKTPFYGESGGQVGDHGLI
                     ENTSFKAEVMDTQKSNGVIIHHVKILSGSCSKNDNVTAQIDQIRRSLIKANHTVTHLL
                     QAALRKVLGDHVKQSGSIVEPERMRFDFTHFEAMTESEIRDVESLVNRKIWENLEVKT
                     SEMPIKEALELGAMAEFGEKYGATVRVVASGDFSLELCGGTHVNRTGEIGIFKILKES
                     SPGAGTRRIEGVTLKGVLDRFNHIDAIKNKVIELTGTNEINVTDKVASIIEENGRLRK
                     EIASLKKADIASLVDQIIEKGFKIEKTICVFHKFDNVDNEVLRDAADRIRQKLNNSIV
                     FFASETQGKVMMLCAASKEAVEAGVDCGGIIKQCAPIVGGGGGGRKEMAQAGGKNPDK
                     IEELFDEVKLKVQKTLGKSTN"
     gene            complement(15110..15748)
                     /locus_tag="P5123_03145"
     CDS             complement(15110..15748)
                     /locus_tag="P5123_03145"
                     /inference="COORDINATES: protein motif:HMM:NF014668.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006282 - regulation of DNA repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RecX family transcriptional regulator"
                     /protein_id="HRX15287.1"
                     /translation="MDRIARISFSGNSIRILFESGLSIPLPVTIEYSSKYSVGDSLDE
                     AQILELKKKSEKHLCRDKALRYIANPHTESQIRVYLKKKEFSNEAIKDALVFLKKMNY
                     INDEAYAKRYLNNMLKRKVVGRKYAENRLKQTGVDKVTIERVIREESLLFDNIDALYE
                     FACKKYESISGKKNCYAKLVYALNSRGFSSSDIFTVLEKMKNSGYTFENNMR"
     gene            complement(15755..16498)
                     /gene="pssA"
                     /locus_tag="P5123_03150"
     CDS             complement(15755..16498)
                     /gene="pssA"
                     /locus_tag="P5123_03150"
                     /EC_number="2.7.8.8"
                     /inference="COORDINATES: protein motif:HMM:TIGR00473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003882 - CDP-diacylglycerol-serine
                     O-phosphatidyltransferase activity [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-diacylglycerol--serine
                     O-phosphatidyltransferase"
                     /protein_id="HRX15288.1"
                     /translation="MNLAWIPNTLTMGNLVFGFLSIVYASKGQFVLSGFLILFAALFD
                     GFDGQVARFLKVESRIGAELDSLADCITFGVAPGYLAYSAYFCDMVITLNYNSIPAGA
                     FLATLFPVCGAYRLARFNVSHDSKHFCGLPSPIAGIAIALVPISANASVFSDTAICVT
                     DYLYIFVPAFVIIALLMISTMSFSKPQAVLLAKLHGIKLAVFIVVLASLLFAFRKYML
                     FIVFSLLFLYVMSALISFVIQIIQDFRLK"
     gene            complement(16605..16688)
                     /locus_tag="P5123_03155"
     tRNA            complement(16605..16688)
                     /locus_tag="P5123_03155"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(16652..16654),aa:Leu,seq:cag)
     gene            complement(16750..>18952)
                     /locus_tag="P5123_03160"
     CDS             complement(16750..>18952)
                     /locus_tag="P5123_03160"
                     /inference="COORDINATES: protein motif:HMM:NF013440.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0032784 - regulation of DNA-templated
                     transcription elongation [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="GreA/GreB family elongation factor"
                     /protein_id="HRX15289.1"
                     /translation="DQAKSVHYLKLALEGYIKNSDVKGINEVWPKIVDKCWDDVSYFD
                     RIDRMLADLQRKDVSVALLKALYEKYKNVDPEIAINYLKKTLNYAPKDNDLRHALISL
                     YKTVYKDHSQLNRFIKLSNLDNTNTPVVAAIKAFENNIIFDSDTYVFHRTWGLGQIVS
                     IDDENVIINFEGKEDHRMSVNMALQSLLALNNDHLYVRQHLEPEKMQKLFKDDVIEFI
                     KIILKSNDDTVSVPELKRDLIPVYVSVKNWSRWWTKVRGLIKKDPSIGFAPNKKDQLV
                     LRENPISYADELLKKFSNADSFSGRLDIAHEFVNNVDVNEGSEQVVYFIDYFSSAASS
                     KSATKLILAYFILSDLEKFSKEKSDLGSIKKQVVSYIEESDDLPIICMKISSYDNKKD
                     FVNLIREHRENWVSIVSKILFEIPVRIHKYIFNLLIIEKEYGTINDFIERITAASKEN
                     PEVFLWVARNILLKSWIYEWLDYSKAGLVLSLFRVYNSIDKSETKGNRLKNMAYEILF
                     ENDLELLHEIVSENEEQFLGKLYDIAANTEHIDESEMDKFEEVIREKFPNFNAERSGS
                     AVIEESEEEKIFVTAAGLERKKKELNHMRNVEMVQIQKGLASTVDVSGDLRENVDYNA
                     LLEQQSIIKKSISKLDSELKVAEIIDPAKADKDVVTIGSVVTLMNKDDNQSMVITILG
                     PWDVDFEKGVLSYRSDVAVTLRGKKIGDEVTLHLGGRESQYVIKELSVYKG"
BASE COUNT         6053 a         4046 c         2991 g         5862 t
ORIGIN      
        1 gtaaatcact gtttttttca ataaaaagat acagctcata atcacataat accttttcac
       61 tggttgtaga aaatttcata aaacacctgc tttaatggtt aatcacgatt ttcaatccta
      121 caaccttatc aaaaataatt tcaatcagtt tttttatcac tccgttcatc aataatctca
      181 tccggtatat cggtaataag tttaaccagc tcgttaaaaa taaaatcgac atccatgaca
      241 gcctttacat tgccgttttt gtcttttatc ggaacagcag cagtaattat caaccgcttt
      301 gtgtatttgg agaaaaacag atccgaatag taggcttcgc ccgtttcaag aacttcacaa
      361 aaccagacat gatttttaaa atctttattc atcagatttc taaacagagc tttctcccca
      421 tgctgcgtgt gaacctgtgt caaacggtat ccatcagttt tggtaacagc cagcatctgt
      481 atggatccct ctttttttac aatcttttca agaaaatctt ccagtttcga aacctcaagc
      541 gattcaacct ccggagctgt tgcaattttt tcagcaattt ttttgccaat tgcgattgcc
      601 tcatttctcg cgctatcaaa ctctgactca aacagcgacg gaataaaatg ttttgcctgt
      661 atcaatagct catccggaga tatgctggtt gtacgcccct gttcatactg ctgcatcacc
      721 caattattaa tatgttttat tcctgggtga cgtttagaaa cctgttcgcg ttctccggca
      781 acaattgccg gtatattttc attaagccat tgcgcaatgc cggccaatcc cgatttgtcc
      841 gttaccatca cccttattgg tctgttcaaa agagcatcgg tatcaaatat attataaatt
      901 tcctgatttt ttaaaatgcc atccgcgtga ataccggcac gtgttgtatt aaaatctgct
      961 ccaacaaatg ggtaatttgc aggtatttcg gccttaactg tctgctcaaa atagcgcgca
     1021 atctcggtaa ttactcttgt atcaatcaaa tcatcaccag tcaggctcat atattcaatg
     1081 acagccgctt caagcggagg atttccggtt cgctctccat acccaagcag agacgcgttt
     1141 accgaagcac aaccgtaaag ccaggctgta gccgcgttaa catgcacttt gtgaaaatca
     1201 ttatgtccat gccactcaag ccattctgcc gggtatccca gtttggaatg aaaggcatca
     1261 accatcttgg gaacactcct tggtaaaaca gcacctggaa aagtgacacc ataacccatt
     1321 gtgtcacaaa gccttatctt aactggcatc tttgcctctt cggcaatacg taacagcttt
     1381 tctgcaaatg gcaggcaaaa cccgtaaata tctgccctgg ttatatcttc aaaatgacag
     1441 cgcggaacaa ttccattctc aatggacgtc tggactatct ccaagtagtc attcatcgtt
     1501 ttttcacgcg tacgcttcag ttttaaaaaa atatggtagt ccgagacaga cgtaagtatg
     1561 ccagtctcct taaggttaaa agattttacc aaagcaaaat ctttcttatc agcacgaatc
     1621 cagcttgtta cttctggaaa ctgatatcct ttctcaagac aaagctctac agcctgcctg
     1681 tctctgtcac tgtatataaa aaattcagac tgtcttataa gccctttttg cccacccagt
     1741 ttatgtagca gtgtataaat atcagcaatc tgtccgggtg tgtatggagg tctggactgc
     1801 tgaccatccc gaaaagtcgt atctgtaatc caaagttctt taggaggaga cggaaaaaca
     1861 gtagaagaat cgaataaaat acgaggaatt ttggtaaaag ggaactgttc cctgtaaagc
     1921 gcaggttcat ctggctcaac taatgagtaa ctactaattg attttagcat tttgccctcc
     1981 attcggacat taaattagca atatcatgcc ttttttgtta attatttgta cagcaatttt
     2041 tatatcttgt catttcaata ttgtaaaata tctgaattct ctatttatat gatttttctc
     2101 aaatatttca ctttttttat gatatttatg taattcgctc gacattaata catgtcacat
     2161 ctatgttgtc ttaaatgcaa aagtattatg catgtcagta gcttaaaatt atcccgcttt
     2221 cctctcccag ttcaagttct tcgggcaaaa ggcatgtcaa tttgaaagtc tacaagcata
     2281 tctttattgt atttaattta tttattatgg agttctaata atggaacaag gaactgtaaa
     2341 atggttcaac gagagcaaag gttttggatt tattcaaaaa gacaacggta acgatatatt
     2401 cgttcaccac acaggtatta atgccgaagg cttcaagaca ctggaagaag gcgcacgcgt
     2461 tgagtttcgt gtagaaaaca gcgaaaaagg cgaaagagcc acttctgtta cacagctgta
     2521 attaaaactt aattgtttaa tcatagacag cacaaaccgg cgtttacgcc ggtttttttt
     2581 gtattaaaat tgcaaaaaaa accaacttga taagaatgtc actattccta tctatcatta
     2641 acttgctcaa attttattta tgattaacta cacaaaggca actatgaata cgactataga
     2701 aagaaaagca gacgcttcag aacgagtgca aagcgctctt gagcattacg atcaaggata
     2761 ttcctgttca caatcaatcg tactcgcttt ttcagacctc tttgatcttg accccaaaca
     2821 tgcaaccctc atctcttcag gtttcggagg aggaatgggg cgtttacgtc gcacctgtgg
     2881 agcattaacc ggcgcttata tgattcttgg tttaaaattc ggaaatcatt cacctgacga
     2941 taacgattca aaacttgacg gttaccgtgt agtacgaaaa ctaacagaac aattcgaggc
     3001 tattcactct accagcgttt gcaaagagct tcttgcgaac cactccaaaa accaaaacga
     3061 cccggccaaa agacaccata aacatatttg ccgcaaagcg atagaggacg cagcccggat
     3121 actggaaagt ttaattacag agtagcatta ataactttgt taattatttt caaaaactat
     3181 ttataaagct cgagcatttc atatgaagaa cagaaaccat gcattgatac ttgccggcgg
     3241 atcgggacat cgtacaggat gcgatatccc aaaacagatg atctcgatta atgatattcc
     3301 cttaataata catactttac gtaaattcga aaactgtaat gagattgata caataactgt
     3361 cgtttctcac aatagttatg ttgaagatat ctataaatta tgcctaaagc acgatatttc
     3421 aaaaattata aaatgtgtta tgggcggaag tacccgacaa gaatcttcat ttttgggtat
     3481 tagagcactt gacgcccgct gtgacgacat tgttattatt cacgatgcgg ctcggccata
     3541 tttaaacgaa tcaataataa aatctgccat caacgaagca cgtgagcacc atgcagcttc
     3601 ggcagttatt tctgtaaccg acacagtcta tattgcccaa aacggatcca tagtgtctgt
     3661 gcctgatcga aaaacagtgt ataatgtgca aacacctcaa tcttttagtt atcaaaccat
     3721 tgttgaagcc cacaaaaagg cattggatga caaaataagt gatgcaactg acgatattat
     3781 tctggctttg aacacaggaa tagagcctgt tttattcgaa ggagacgtta ccaacaaaaa
     3841 aataacaacg aaggaagatt tggaagaagc acaacggctg cttttttaaa ggggtaaatc
     3901 atctttgttt ttaaactcgt ttaagttgta aaagagggac tgcttacttt tactatgttg
     3961 tttattctct tcactgattt cacgatattc attgttaaat atttttacaa aagtatctat
     4021 tctttcctgt ttatcctgaa attggattgc cacctcgcgt ccttcaactc tgacaaccac
     4081 accctttaaa agcatttttc ggcttttgaa gggaaagagt attctaatct cgtctttttt
     4141 atacagcaca gaatctctta agaaacacat gccacctgca ctcacgttta aaacctctac
     4201 ggtaaaaaca ttatcatcgt tggccagctt atactgtaca aaaaaagctt ccgtacttat
     4261 tctgtctgta tcacgcttat caaaagctcg agacatctta acctctattt atcaaagcaa
     4321 tctcattgca acaatcaacc cgcggacaat ttacgcaatc tttccatatt ttctcaggaa
     4381 aataattttt atcgattaat tcaaaattca atttgctgaa aaattctgga acataggtca
     4441 aagcaaaaat tcgtgattgt ggctgccgtt ccctaagcag agttacaaca tgattcacta
     4501 aggcacttcc gattcctgat ctggcatact cttctgcgac aacaagtgaa cgtatttcaa
     4561 aaaggttgtt atcgtaatca tgataagaca caaccccagc tgctttttca ttatcacggg
     4621 caataaaaaa agcgcccagc tcagccataa tatcttcacg tgatcgctca agaacaattc
     4681 cctttttaac ataaggcgct aaaagattta aaatatcgcc tatatcggac tctttgattt
     4741 tatctatatt taccattcat tattaatcag cgttttaaga tcaaagcact atgaaacagc
     4801 ttgttgtccc tgtaataatt gatcctgttt agactcaatt cggcttctct tcggtttttg
     4861 taaggccccg cgtaaattct aaaaaaacct tcttgtctcg caagaattac agtatgtcct
     4921 tttccactta aataatccgc tatctcctga gattgatttg cagaaaactt attacctata
     4981 cgaataacaa acggatactc ctctttcgga aaagaggtta ttgctgattt agtatcggaa
     5041 ttcttaccag caagactctc ggcttcggca gcaggaagat cctctgtaga accctcactt
     5101 tcattattgc caactatatc cgtaacttta tctcctatgt cactcatagc ctgacgagtc
     5161 acaggatcaa ctatttcttt ttccgaccca ccgctaaact tgatacctac aaccaagccg
     5221 gatgtaaatg aaataataca taaagaaaga accaaaacca aaaaccttgt tgccctgttt
     5281 ttttttcgga tcccctcttt ttcataactt cttgccattc ttgaaaaata ttctggatcc
     5341 ctggatataa aatcaggtat attaggctgt tgcgcattac cggaataaaa atctatgttt
     5401 tgcatacaaa tcacctgttt aagcaaaagt aagctcatcg ttaacgcgaa aatgagacat
     5461 atacttatat tttcggtata tgatgaaaaa cagttagatt tttttattat gtcatcaaca
     5521 aaaagaacag tcaatagtgt taaatccgga ctaaacccta ttatttatcc ttatcaaaat
     5581 attttttcag tttttttatc tctattggcc ttaaatccct gaacatgcct ctatgcagat
     5641 tacccagaac aagaggcccc agcgaaattc taataagcct tttgaccttg taatcaaact
     5701 ttttgaatga gactcttatt tgccgctttt tcccctctga aatggtcata tatactgaat
     5761 gtttctgttc acttatatat tcaatggagc atggaccagt tttgaactcc cgtaatcgaa
     5821 gccctttctc aattttagcc ttatcttctt cttccaaaga gcgatcgaga gaaacgatat
     5881 acttcttctt acattcactt gagggatgca tcaatgcttg cgccaaaaat ccatcgtttg
     5941 taagaagcaa aagcccttct gtatctttgt caagcctgcc aacggggaaa acaccagcct
     6001 tccagtattt atcatgaatc aaatcgacaa ttgtaggcct tcctagaggg tcacccacac
     6061 tggtaatata acctttcggc ttgtttagca taacataata gagaacccct gattgcacaa
     6121 caggttcacc cttgtatgtt acaagatcat tttcaggatc caccttgcat gcaagagtca
     6181 cagactcgcc attgaccctt acatcccctg cttcaataaa tttctcaacc tttcggcgcg
     6241 aacccagacc gcaacaggct aaaaatctgt ttaaacgcat tttttcagcc atcagttata
     6301 tttccgcgtc atatgattct ggcttctctt cgtcatcagt ttcactagaa atcttcttga
     6361 atctgtattt atagtcctct gtctcttttt ctatcaatat ttctactccc ctctgcacaa
     6421 gggtcagatc gttaccaaaa gtagctccat ccgaaaaaat tatcagattt ttatccatat
     6481 caagagaata ctcacctttc tttacaacac cgccgcgtga agatctatat tttttgtcct
     6541 catacaaaat caaagaatgg ctggtaaagt tacctgaaga tgtaacactc caccattttc
     6601 cggtaagctc cttaacaatt ctctctatat tttcctgagc aacctgtttt tggtaatctt
     6661 caattgaaaa accatcaccc tctttcaaaa tccttatatt tgaaccgaaa gtccatccaa
     6721 caatacctga agatagctga acgtaatacc agtaatcttt gattcgcgca attcgggact
     6781 cttcagccga tttcattata atctttacag tatcaccctt cataagataa tctgtgcggc
     6841 tcgaatgtat ctgcggctga aaccgcactg ccgaataatc attaagagct accgcaaata
     6901 cttctttggt ttcaatatcc tcatcgatgg ttgaatcact cttatccacc gcacaagccg
     6961 ttagcaatat aatcaaccac aaaaacatac ttcttttcat tacttactcc cataaatatg
     7021 ctacatttta gctacaaata cctaatttta aaacaattgt tttgactttc atattggtca
     7081 acataaattt ggaactcaac agggctcttc acaatactgt ataaattatt cgattttatt
     7141 attaatctgg caatatttta tttaaaaatt tgaccattag caaatccgat tctttattgc
     7201 ttctaactgc cgattacaat attttaatca tctatagttg acatcaaaat attacaaaat
     7261 tattttgttc aaaagccaaa tttatgccga ttatataata attggtgctt ttttatatat
     7321 aaatattata tttttgatgc atgatatcgg taataattac aaattaccgc tctttgtgtc
     7381 ctaacaaacg attatgacaa tactgcataa gaaattatca gataaatgct atggaaacca
     7441 ggttatataa tggcaaaaaa atacaatatt tcaataggtt ttaaacttat tactctgatg
     7501 tccctcttga ttactctatc acttggggga ataatatatt ttgccaccta ctattttcgc
     7561 gatgataacg aaaaaagagc tgaagaggtc accttagatc gtgctgaatt aatcgccttg
     7621 caaatcagaa ccgactttaa atcaataatt ggaaacagca ctctacttct tgaaaaatcg
     7681 ctactactca agacaaaaac cgaaatttca gatacagacc tactctacat tgcccaacta
     7741 actgatgaaa actcaccagc tattacacaa cagttattaa acgacacagc tctacttaca
     7801 atggacgcaa caatggtttc tcccgactct gttgtaagcg cagaaattga tgcaataaag
     7861 ggcggattta acaccgagac gaaagtattc aacggatcct tatactttaa acgccctgtt
     7921 attggtataa ttacacccca tattaccgaa gaacctggca gagcatcttc catacttctg
     7981 atattcatat ccatgaacag atttcttgag gcagtcaaaa cacagaccat ttaccaaagc
     8041 tttattgtta acggaacagg tgaacttatt gcacaccatg accctgaact tgtaacggca
     8101 aaagctaact tcatgaatat gcctcttgta agcaccatga ttgagagtac cattgatttt
     8161 ggcaaaaagg cttttaaaga tgaagaaggt aacagtttta taggatcgtt taaaaaaaca
     8221 ggtttttcag acataggggt cgtaacttat gtagatgaat caactgcttt tgccgcagtc
     8281 aaaaatcttc aggaaagaaa tattttacta acgattatag ttataaatat cgccattatc
     8341 tttcttttta tcttttccag aactctaaca aaacccgtta aagcattatc agctgcggcc
     8401 aacaagatta aaaacggaga atttgatatt cagctcaaac cgacaacgcg ggatgaaatt
     8461 ggttacctaa cagaatcatt tggagaaatg gctcttgggc tttcagaacg tgaaaaactg
     8521 aaaaacgcac tgggcaaatt tgccaacccc gagatagcgg aacaggcgct taaaggagaa
     8581 attcagctgg gtggagaaag aaaacacgca gcaattttct tttcggacat aagaaatttt
     8641 acctcgatat ccgaaaaact cgaaccggaa gaggtagttg aatttttaaa tgaatatatg
     8701 agtcttatgg ttaactgtgt taacaagaca cacggtatgg tagataaatt tattggcgac
     8761 gccattatgg ctctttgggg cgtacccttc tctcatggca acgaccttca aaactccatc
     8821 gactgcgcac ttttaatgag aaaagagctt attaaattca ataaaaaccg cggtggagat
     8881 aaaaaaccaa taatcaaaat tggttgtgga ataaacgttg gccccatggt agcaggtcaa
     8941 ataggttccg atgaaaaaat ggagtacacc gttattggtg acaccgttaa cctagcatct
     9001 cgaatcgaat ctcttaacaa accttttgga actgatattt taatttctga agatgcttat
     9061 actgtagtaa aagaccgtta tttaactgta cctatgcggc aaatactggt aaagggaaaa
     9121 gaaaaagctc aacaaattta tgccgtactc tcacataaag acgatccgaa tggcattaaa
     9181 tcaattgaag agttgcgaaa tgttcttgat atcaccgaag ttcaaatatc tgactctgat
     9241 ttccctatcg aaaatgaaca aaaatttgaa atactgaaca acaaccataa agattcaaaa
     9301 agtcacgaaa aactatttaa taagaaagat gtgctctatg aaatccttga ttaaagaata
     9361 cacattacct ttttttaacc ttatagcaat aataattgcc gcttttttgc tgtatttaaa
     9421 ctacacgtat accttgacac actcgtctac cgttcagatt ggcacgatta tattcaaaca
     9481 aaaaatcgca gaaagaagat actctcaaca agttatctgg gaagggatta ccaataactc
     9541 gccggtatat aacttcgacg ccattcgcac cgacaaggga gctcttgccg taattgaact
     9601 ggataacgga acatctatcc aactcgaaga agaaacaatg ataataattc aacaatctga
     9661 tcttggactg gatattgatt tcgagaatgg atcaattaac gcacaaaaaa gtgatgatga
     9721 ttcctcactt aacattcgtt ctggcgatac agtagtagct gttaacgacg gaagcctcaa
     9781 tgttaataaa aatgccggca cacgggatct taaccttaac gtttcatctg gcaccgcagc
     9841 tttaaattca ggtggcaaaa tatcacaaat tgacagcaca aagattgtaa atatttcttc
     9901 tggtgaagcc aaaatcggag ataaacagat tattttacaa tcaccggctc ctgctaaaac
     9961 attcatcacc gcaaatcccc tggatattaa tttcaattgg acaagttcca aagaatcaac
    10021 ttcaaacttg caaatttcaa aagataaaaa cttcagtaac ataatgtata cagtaccttc
    10081 tgcaacaggt aaagcgacag caagacttga aaatggcaca tattactggc gagttacact
    10141 taatggaaca aacagtgaaa cacgccgaat ctcggttatt agagagacta ctcctgttat
    10201 tttaagacct ttaaacggac aaagtttcaa ctataccaaa aaacctcctg ttatcatttt
    10261 taaatggaat ggtgccgaca cagcctctca atacacggta gagattgccc gaatggaatc
    10321 aatgaatact ttacataaaa gcataaatag tcttgaatcc tttatttcgg tttctgatct
    10381 tcaacccgga aaatattatt gtcgtttgaa agcacgttac gcccttgctg accgcaaaca
    10441 cgaactattg agccctgtta tctcatttac tgttaatcaa aagtcccaac ttgatgcacc
    10501 taaacttttt tcaccagtta atactttaat taatgccaac aaaaaaccca cagtttttaa
    10561 ttggcaacct gttggcggag ccgataaata taaaattgct atttctggct catccggacc
    10621 tcttttttct gctataacaa catcaaatta ctttgcttat aatactcttc ttaatccagg
    10681 tgactacaca tggagtgtta ccgctatgga taccgaagga gctgaatctc agcttaataa
    10741 ttctgcaaaa tttactgtat cttcaactgg aaagataaca acaatatcac catcttcatc
    10801 aagtcgattt aaaacaggtg aggccgtcaa tctgacatgg agagacgaaa acaatggaaa
    10861 caactatctt gtcataattt caaaaaactc tctttttgcg acaacttaca gagagcgaaa
    10921 ggtctcttca agatttacaa ctttttctga tcttgaatct ggtaactatt actggaaggt
    10981 ctatctgctt aacgatgacg atcgccgtct tatcgccagc aacgctgatt ctttcagtat
    11041 atacggaaac gtagttcgtc ctattcctgt ttctcccata aatacaaagt ttccacacga
    11101 cacatcgtcc atctctttga gatggaaacc agttccagac gcgactcatt acgagataga
    11161 aatatatcaa gaatcatcac taatatcaaa attattaaaa agggctacaa cagcctctac
    11221 ctcttattca cttaacattg catcttacgc tccgggtagt tattcatgga aagtcagatc
    11281 tatccaaaaa agtggctctg ttattgtcac cagcagtgat ttttcaaaag aggttttcac
    11341 aaaagaatca ttaaaagagc cggaaaagaa agagtataaa attgagattc ttaatccgtc
    11401 aaaaatatat atagaataag agattgcctt acaatcagct gacattggat attaataatg
    11461 aattgttata gaagacatat tatactacgg ttaataaaac tgccaagaca ggtattattt
    11521 atgctgcttt tggggtttat ctatttatcc aataattcta tactgttatc taaacccaaa
    11581 atcgaaaaaa ctccacgcat tgaatggcga gccatttcag ctgcttctag atacagggta
    11641 caaataacca aaagcggttc tacaattatt gacacaaaaa cagtttccaa ttttatatcc
    11701 gcaccgttac atcatggtgt ttataaattt aaaataaccg catacaacca aaaaggcgaa
    11761 acaattggtg aatccggctg ggaaccgcta ataattcaaa gctggagtct tgtaagcaaa
    11821 cagtactatt atgttggtgt cggtgttggg tttgagtaca taactcctag ttggaacaag
    11881 ataaacgaaa atggtaatag ttggaacgtt tatggaggtt acgacttacc cattcaaagc
    11941 atacagctcg aagcaatact agattatcag tctttctctc ctatttataa agaagataag
    12001 gttagcaccg aaatagatat gtactccctt aactgtggag tgttctattt ctcacgactt
    12061 tctgatcttt ttactgctgg agtcaggtta tcacccggat tatcatatac aactataaca
    12121 atagaagata ctgaagaaac taaagaatat aattctatag gtttatcagt tattcttggt
    12181 attggtttaa gatttaatat gagctacgga tttattgaac atggagttga atataaacag
    12241 acatttctaa atggggagga tttcatgtcg gtccgcccct atttaagagg cggagcccgt
    12301 ttttaacttg ttatagtaca caaatgcttt tgttgtgctg gggaactccg gaaaagtaca
    12361 atgttccgga gggtaacatg gttaaaatgc ctactctaaa ggaacttttc ggatgatcct
    12421 ttgctggtcg tccagcacca tttttataaa gttaatttat atgccttaac ttaattagta
    12481 gattttccta atgttttttg aaccttcaac ttaacttcat caaaaagttc ttcaatttta
    12541 tcggggttct tacctccagc ctgagccatc tcttttcgtc cgcctccacc gccgccgact
    12601 attggtgcac attgtttgat tattccgcca caatcgaccc ctgcttctac cgcttcttta
    12661 gaagccgcgc ataacatcat aactttccct tgagtttcag acgcaaagaa cacaatactg
    12721 ttattgagtt tttgtctaat tcggtcagct gcatccctta gcacttcatt gtctacatta
    12781 tcaaacttgt gaaaaacaca aattgttttt tctattttaa aacctttttc aattatttgg
    12841 tcaactaacg aggcaatatc agcttttttc aaagacgcaa tctctttacg taatcggcca
    12901 ttctcttcaa ttattgaagc gaccttgtct gttacgttga tctcatttgt accagtcaac
    12961 tcgataacct tgttcttaat cgcatcaata tgattaaacc tgtccagaac tcccttcaag
    13021 gtaactcctt cgatacgtct ggtaccagct cctggtgatg actccttcaa tatcttgaat
    13081 attccaatct cgcctgttcg gttaacgtga gttcctccgc aaagctcaag actaaaatct
    13141 cccgaagcta ctaccctaac tgttgcacca tatttctcac caaactctgc catcgctcca
    13201 agttcaagtg cctctttaat aggcatttca gaggttttaa cttccagatt ctcccaaatc
    13261 ttgcgattta caagactttc aacatccctg atttctgatt cagtcatcgc ttcaaaatgt
    13321 gtgaagtcaa atcgcatgcg ttccggttca acgattgatc cggattgttt aacatgatca
    13381 ccgagcactt ttcttaaagc agcctgcaga aggtgtgtca ccgtatggtt cgccttaatt
    13441 agtgagcgtc taatctgatc aatctgagct gtaacattat catttttcga acaactaccc
    13501 gataagatct ttacgtgatg aattattacg ccattacttt tttgagtatc cattacctcg
    13561 gctttaaaag aggtattttc gataagccca tgatctccaa cctggccacc cgactcgccg
    13621 taaaatggtg ttttatcaag aacaatatag ccttccatgc cttctgataa tacttctttc
    13681 aattcgcttc cggaggccaa tacacaaatt tttgattctg tactataatc actataaccg
    13741 gtaaaaacag attcttcaat tccgttacaa atttcactta ccagcttttc gtaagatccg
    13801 tccgccgatt tccaactatc ctttccgcgt tgtcgctgtt tctccatctc ttttgagaaa
    13861 gcatccatgt ctacagtaac accctcttca agagcaattt ctgttgtcat ttccacagga
    13921 aaaccaaatg tatcatatag ctggaacgcg tcctctcctg acaatatcga tccggttttt
    13981 tttgcgtcaa ctataagctg ctcaattctt aaaattccat tttcaagagt ttccagaaag
    14041 cgtttctctt caccttcaag aaccttttta atattcatgg aagccgagac aatttcaggg
    14101 taaaactctc caaaaatctc gacaattaca tccaccatct tgtaaataaa tggttcatca
    14161 acccccaaag ttctggcaaa acgcaatgca cgacgcaaaa ttctccttaa cacatatcca
    14221 cgaccctcgt ttgagggata ggcaccatca gccattgcaa aagtcaaagc tcgtgcgtgt
    14281 tcgacaatta cattaacagc aacagctttt tttcccttat actcaacacc aagattatca
    14341 caaacaaact ctatcattcg tgtaaattca tcagtttcat aaatagatga tacattttga
    14401 gtaatcgttg ctagtcgctc tagacccata ccagtgtcaa ttccggtttg cggtaatgga
    14461 gtttggttcc cgtccgtatc ctggtgaaat tgattaaaaa ccagattcca gtactccata
    14521 aatctttcac aatcacaacc aggtttgcaa tctttatcac cacatccatt ttccaagccc
    14581 ttatcaatat aaagctccga acatggtcca caagctcctg aatctccagc cggtccccaa
    14641 aaattgtcct ctttaccaag acgaacaatt ctttcagccg gtattccaac aattttattc
    14701 catatatcaa aagcttcatc atcatccaga taaaccgaaa cccaaatttt ttccggatca
    14761 agtttcaaca ccttaaccga atactcccaa gccatttcga tagcttcttt tttaaaataa
    14821 tctccaaaag aaaagttacc caacatttca aagaatgtgc aatgtctttt ggttcttccc
    14881 acattttcca aatcacttgt acgaagacat ttttgaacag atgctgctct tttataatcg
    14941 gttttgacaa tcccggcaaa cattggttta aattgaacca taccggccgt agtaaataag
    15001 agtgttgggt catccttggg tataagcgga cttgaatgaa tttgtcgatg acctttagat
    15061 acaaaatatt caataaaaga agctcttaac tctttaacaa gcacaatgat tacctcatat
    15121 tgttttcaaa agtatatccg gaatttttca ttttttccaa aacagtaaaa atatcagaac
    15181 tcgaaaatcc ccggctattt aaagcgtaaa ccagttttgc ataacaattc ttttttccac
    15241 tgattgactc atatttttta caagcaaatt cataaagcgc gtcaatatta tcaaacaaaa
    15301 ggctctcttc cctaataact cgctctatag ttactttatc aacaccagtt tgtttcaatc
    15361 tattttcagc atactttctg ccaacaactt ttcttttaag catattgttc aaatatcgct
    15421 tagcatatgc ttcatcatta atataattca tcttttttaa aaacactaaa gcatctttaa
    15481 tcgcctcatt tgaaaactct ttttttttca aatatacgcg aatctggctt tcggtatgcg
    15541 gattggcaat ataacgcagt gctttatcgc gacacaaatg tttttcagat ttttttttaa
    15601 gttccagaat ttgagcttca tcaaggctgt ctcctacaga atattttgat gaatattcta
    15661 tggttacagg aagaggtata gacagtccgg attcaaacaa gatccgtata ctgttaccag
    15721 aaaaagatat ccgcgcaata cgatccattt attactattt taaacggaaa tcctgaataa
    15781 tttgaattac aaagctgata agggcggaca ttacatagag gaaaagaaga ctaaacacaa
    15841 taaataacat atatttgcga aaagcaaaaa gcaggcttgc caaaactaca ataaaaactg
    15901 caagtttaat tccatgaagc tttgccagca ggacagcctg tggcttagaa aaactcattg
    15961 tagatatcat taaaagtgcg atgattacaa aagctggaac aaaaatataa agataatcag
    16021 taacgcaaat agctgtatca gagaaaaccg aagcattagc actgatcgga accagagcta
    16081 ttgcaattcc agcgattggt gaaggtaacc cgcaaaaatg tttcgaatca tgtgaaacat
    16141 taaatcgtgc caaacgataa gctccacaga cagggaaaag agtagccaga aaagcacccg
    16201 caggaataga attatagttt aatgtaatca ccatatcaca gaaataggca ctatatgcca
    16261 aatacccagg agcaacacca aaagtaatac aatcggctaa tgagtccagt tcagcaccta
    16321 tacggctttc gactttcaaa aaacgggcaa cctgaccgtc gaaaccatca aaaagagccg
    16381 cgaaaagtat aagaaaacct gaaagcacaa actgcccctt ggaagcatac acaatcgata
    16441 aaaacccaaa aaccagattt cccatcgtta aagtattagg tatccaggct aaattcatgt
    16501 agagctccca atattaaaaa tttcaggtca taattccaaa ataatttgca gctttttatt
    16561 tggtaatctt tcattgattt ataaatcaat tttataatac aaaatgccgc gagagggatt
    16621 tgaaccctca caggcgaatg cccaccagac cctgaacctg gcgcgtctac caattccgcc
    16681 atcgcggcaa caataatttt ataactatag atttatcata ttttatgtca agatgtttac
    16741 caattcaaat caacccttat aaactgataa ctccttaatt acatactgac tttctcggcc
    16801 tcctagatgg agtgtaactt cgtcaccgat ttttttacca cgcaaagtaa ccgcaacatc
    16861 tgaccgatac gacaacacac ccttttcgaa gtccacatcc catggaccaa gaatagtaat
    16921 aaccatggat tgattatcat ccttgttcat cagcgtaaca acagaaccga ttgtcacaac
    16981 atctttatca gctttagccg gatcaattat ttctgccact tttagctctg aatcaagttt
    17041 ggaaatggat tttttaatta ttgactgttg ttcaagtaaa gcgttataat caacattctc
    17101 gcgcaagtca cccgacacat cgacagtact tgccaagcct ttttgaatct gaaccatctc
    17161 gacatttcgc atatggttta gttctttttt cttacgctct aatcctgctg ctgtaacaaa
    17221 gatcttttct tcttcacttt cttcaataac tgcagaaccc gatctttcag cattaaaatt
    17281 aggaaatttt tccctgatta cttcttcgaa cttgtccatt tcactttcgt caatgtgttc
    17341 tgtattagct gctatatcgt acaacttgcc caagaactgt tcttcgtttt ctgatacaat
    17401 ttcatgtaac aattcaaggt cattttcgaa taaaatttcg tacgccatat ttttaaggcg
    17461 attgccttta gtctcggatt tatcaattga attataaacc ctaaaaagag atagcacaag
    17521 accagcttta gaatagtcaa gccactcgta tatccagctt ttcaaaagaa tatttctggc
    17581 aacccacaag aagacttcgg gattctcttt tgacgcagcc gtaattcttt caataaaatc
    17641 attaattgta ccatactctt tttctataat tagcaaatta aatatgtatt tgtgaattct
    17701 aacaggtatc tcaaataaaa ttttgcttac aatacttacc caattttcac gatgctccct
    17761 gataaggttt acaaaatcct ttttattatc ataggaagat atcttcatgc aaattattgg
    17821 caaatcgtcc gattcttcaa tgtatgaaac tacctgcttt ttaatactac ccaaatctga
    17881 tttttcttta ctgaatttct ccaaatcaga taaaataaag tatgccaata tcagttttgt
    17941 cgcggattta ctagacgcgg cactggaaaa ataatctatg aaatacacta cctgttcaga
    18001 accttcgttt acatcgacat tattaacaaa ctcgtgcgct atatcaaggc gcccactaaa
    18061 tgaatccgcg ttagagaact ttttcaaaag ttcatccgca tacgaaatcg gattttcacg
    18121 caaaacaagc tgatcctttt tatttggagc aaaccctatc gatggatctt tcttaataag
    18181 accgcgtacc tttgtccacc atcttgacca gtttttaaca cttacataaa ccggtatcaa
    18241 atctcttttt agttcgggaa cgcttaccgt atcatcattt gattttagaa taattttgat
    18301 aaactcgata acatcatctt taaaaagctt ttgcatcttt tcaggttcta gatgctgtct
    18361 tacataaaga tgatcattat tgagtgctaa taatgactgc agagccatgt ttaccgacat
    18421 acgatggtct tctttaccct caaaattgat aataacattt tcatcatcaa ttgatactat
    18481 ttgacctagc ccccaggtgc gatgaaaaac atatgtatct gaatcaaaaa taatattatt
    18541 ttcgaaagct tttatcgctg ccactactgg agtatttgta ttatcaagat tcgataattt
    18601 gataaaacga tttaactggg aatgatcttt gtatacagtt ttatataacg aaatcaatgc
    18661 atgacgtaaa tcattgtctt ttggtgcata attgagtgtt tttttaagat agttaattgc
    18721 tatctcggga tctacattct tgtatttttc ataaagagct ttcaataaag ctacagacac
    18781 atcttttcgc tgaaggtctg caagcattct gtcaatacga tcaaaatatg atacatcatc
    18841 ccagcattta tctacaattt ttggccaaac ttcattgatt ccttttacat cactattttt
    18901 aatataaccc tcaagcgcca atttcaaata gtgaacactt ttagcctgat cc
//
LOCUS       DAOTGZ010000026        18836 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_3064
            NODE_3525_length_18836_cov_5.391779, whole genome shotgun sequence.
ACCESSION   DAOTGZ010000026 DAOTGZ010000000
VERSION     DAOTGZ010000026.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868088
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 18836)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 18836)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:22:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,785
            CDSs (total)                      :: 2,751
            Genes (coding)                    :: 2,732
            CDSs (with protein)               :: 2,732
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 12 of 19
            Pseudo Genes (internal stop)      :: 5 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18836
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3525_length_18836_cov_5.391779"
                     /isolate="AMR_MDS_3064"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1269
                     /locus_tag="P5123_03165"
     CDS             <1..1269
                     /locus_tag="P5123_03165"
                     /inference="COORDINATES: protein motif:HMM:NF015568.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit IIC"
                     /protein_id="HRX15290.1"
                     /translation="ALVFGIKGSTALKSALTLGIAFIGIFMTFDFFVKIINPVMQAII
                     ERSGLNLPVLDTGWPPLAGITWSYSLAPLILFILIVINIILLVGRLTKTVNIDIWNYW
                     HVIFLATLIHHTTENPWLAIIIASAAFILVLKLAEWSAPLVNKLTGMNGICIPHLSGI
                     VHYPIAVVINLLLDRIPGLRSVKANPEHLKKKLGLLGEPMIIGLIIGILLGLAGGYEF
                     RDILNLSFGFAAVVFILPKMGEILGSSLIPISEGMKLFITRKFSHLGATFIGLDVAVL
                     FAVPAVLVTALLLMPVSIILAIFLPGINFIPIGDLTNLLVPVAFIAVATKGNIIKSFI
                     IGIPVVIINLYYASMFAPIVTDMARVSNYKIEGYDGVFTSFLDAGNPLRSWIVLLLEK
                     DILSIALVPVVILMVFITWKSSSSQKIKNK"
     gene            complement(1475..3022)
                     /gene="rseP"
                     /locus_tag="P5123_03170"
     CDS             complement(1475..3022)
                     /gene="rseP"
                     /locus_tag="P5123_03170"
                     /inference="COORDINATES: protein motif:HMM:NF014244.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RIP metalloprotease RseP"
                     /protein_id="HRX15291.1"
                     /translation="MNPDFFVYILWAGLLLGCSIFVHELGHLLGGKMVGIKARVFSLG
                     YGKGFIKKKWGDTTYQITLIPFGGYCAFYGDVPGATLEGKKFEFFTASPWRRIVTVVM
                     GPLFNLFFGIILFFALNLAGYEVETNRIMIPESSSMELPAQKAGLKSGDTVVAIDDKE
                     ISSFMEIQSAVVFSDGKPLNFTVARDGKKINLKVTPVQYSNGGYYSIGIMPYGTDVLI
                     TGLEPSDVAQKAGLQVYDRVKAINGESLKGSGHFSEILQNNGEVPLVLQIERSGENMD
                     IEITPRFREQLVLTEFHSTIFPDESFDINVSDLDTIKTAIKKGTVSVNGKNVTDFDDF
                     LSIIESLEGGKAVIDTKGGSYEAVVKYRKSGFIGVATFAAPEMIPVKHGLAGAFKKSL
                     TDPYDFIVQNLQGMKMLFTGKISAKDNVSGPVQIAKLAGDTMRYRGVREYLLFMARIS
                     IILMIMNLLPIPAVDGSHIVFYLIEAVRGKPLPAGIMEKVQAFGFFLLIGLGVLVLFN
                     DISKLVF"
     gene            complement(3105..4265)
                     /gene="dxr"
                     /locus_tag="P5123_03175"
     CDS             complement(3105..4265)
                     /gene="dxr"
                     /locus_tag="P5123_03175"
                     /EC_number="1.1.1.267"
                     /inference="COORDINATES: protein motif:HMM:TIGR00243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0030604 - 1-deoxy-D-xylulose-5-phosphate
                     reductoisomerase activity [Evidence IEA];
                     GO_process: GO:0019288 - isopentenyl diphosphate
                     biosynthetic process, methylerythritol 4-phosphate pathway
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-deoxy-D-xylulose-5-phosphate reductoisomerase"
                     /protein_id="HRX15292.1"
                     /translation="MPSDVIILGSTGSIGCSTLRVIDEYPDLFSVKGLVCNCNVQTLG
                     EQLERYKPRFCGITSPRDDAQIESLKICFPKTRFFTGDEAALDVVSEGADICVSAIVG
                     AAGFLPSLYALKSCKRIALANKETLVMAGDLFFEQAASCETEIIPVDSEHGALFALLD
                     CMEPEHIDSVILTASGGSLRDYPLEKLAHVTPEEALAHPTWNMGSKITIDSATLMKKG
                     FEVMEAHHLFSLPYERIKVLVHPESLIHSLVQTTCGALHAHLGIADMAFPISYAMLYP
                     RIRPNNFEKLDLVKAGSLTFRELDKRRYPALDLCYNAGRAGGAATVVLNAANEIAVHS
                     FLSHRIGFSSITEVTLHAVDSLCNLPASDVQSVLDADRLARETAREYIDKME"
     gene            complement(4266..5150)
                     /locus_tag="P5123_03180"
     CDS             complement(4266..5150)
                     /locus_tag="P5123_03180"
                     /EC_number="2.7.7.41"
                     /inference="COORDINATES: protein motif:HMM:NF013327.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidate cytidylyltransferase"
                     /protein_id="HRX15293.1"
                     /translation="MTSRTWDTFQRIISALIALPVYFFLIFFDQYYTLGILLISLAVT
                     LSCLYEFYQIASSKGGGTPFIIPGLAAGFVINVIIYMFSFGRVYGFERYLAIFDVRIV
                     LAIFVFLGVIISALHIVIRPLSDGIFSLATTMFGIIFIVLPFSHIILMKALPDGIYYI
                     FILHLVIMFNDTFAYFGGVFFGTHKTGFPVSPNKSWEGYFSGMLFSTVVIIIANEAFQ
                     IFFDRHLFSLPEAILLGMLLSLLGNIGDLVESAIKRDAEIKDSGRIIPGHGGMWDVFD
                     ALIFVFPVFYYYLKLKGV"
     gene            complement(5147..5863)
                     /gene="uppS"
                     /locus_tag="P5123_03185"
     CDS             complement(5147..5863)
                     /gene="uppS"
                     /locus_tag="P5123_03185"
                     /EC_number="2.5.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008592728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyprenyl diphosphate synthase"
                     /protein_id="HRX15294.1"
                     /translation="MPSLKDSVNKSAVPRHIAVIMDGNGRWAKKRGLPRADGHRKGAL
                     VIETLMDAAMDLSVGCVSLYAFSTENWSRPSTEINALWELLCSFFAEKLPVMHQKGIR
                     VYHSGSKKRLPARVCSVIDKAVKQTAGNKGLKMNFCLNYGSRQEIVDAVNNWSAKAKP
                     GEKFTQKKMAENLYVSDLPDVDLLIRTSGECRISNYLLWQCAYAEFIFLKVLWPDFKP
                     SHLYRAVIEFQKRNRRFGGL"
     gene            complement(5863..6420)
                     /gene="frr"
                     /locus_tag="P5123_03190"
     CDS             complement(5863..6420)
                     /gene="frr"
                     /locus_tag="P5123_03190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011344678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008135 - translation factor activity, RNA
                     binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome recycling factor"
                     /protein_id="HRX15295.1"
                     /translation="MVNDILKDVESRMSKSVKALEKEFVAIRTGRANPSILDSVSIDA
                     YGSPMPLNQVATVSCPEARLIMVQPWDKGNIGAIEKAILKADLNLNPSNDGNVIRIQI
                     PELTEERRKELVKQAKNKAEDCRVAIRNVRRDGNDMVKELEKDKSISEDDSKKEQDNI
                     QKLTDKYTEMVQKLTDNKEKEILSI"
     gene            complement(6472..7188)
                     /gene="pyrH"
                     /locus_tag="P5123_03195"
     CDS             complement(6472..7188)
                     /gene="pyrH"
                     /locus_tag="P5123_03195"
                     /EC_number="2.7.4.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015413303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033862 - UMP kinase activity [Evidence
                     IEA];
                     GO_process: GO:0015949 - nucleobase-containing small
                     molecule interconversion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UMP kinase"
                     /protein_id="HRX15296.1"
                     /translation="MSEANPYKRVLVKLSGEVLAGSASAGLDPETVKKVASEVVLAAE
                     SGVQIALVIGGGNIFRGGLGESLGMDRVTGDHMGMLATVINSLALADSISSAGRNAVV
                     MTSFAMETVADYYTVRKASALLADNKIVIVAGGTGNPYFTTDTAATLRAVELKCDLMM
                     KATKVDGVYDKDPAKHSDAKKIDKISYIDVIQNNLRVMDLTAISLCMENMLPIRVFNL
                     FRDNCIKDALNGYAGGTDIL"
     gene            complement(7191..8033)
                     /gene="tsf"
                     /locus_tag="P5123_03200"
     CDS             complement(7191..8033)
                     /gene="tsf"
                     /locus_tag="P5123_03200"
                     /inference="COORDINATES: protein motif:HMM:TIGR00116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation elongation factor Ts"
                     /protein_id="HRX15297.1"
                     /translation="MAEITASMIKELRERTQAGMLDCKKALTECDGDMEKAAEFLRKK
                     GLAAANKRAGREAKEGLVFVKLSDDGKKGSIVELNCETDFVAKNEEFHNLGNKITSEI
                     FDNSLKSGAEIPAALDETIKGAIATTGENMGIGSFDTIEIDKGAIASYIHSNGKIGVL
                     VALASDASDSAILCELGRELAMQAAAAFPQYVTSDEVPETVKDKEREIYKEQMKNSGK
                     PENIIDKIVEGKLSKFYSDVCLVDQVFIKDSAKKVSDVVKEYSTKAGSPIKIEKFIRV
                     QIGG"
     gene            complement(8151..8948)
                     /gene="rpsB"
                     /locus_tag="P5123_03205"
     CDS             complement(8151..8948)
                     /gene="rpsB"
                     /locus_tag="P5123_03205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005398068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S2"
                     /protein_id="HRX15298.1"
                     /translation="MAVITMKELLESGVHFGHQTRRWNPKMQQFIFTSRNGIHIIDLQ
                     KTMQRLKATYSIMKDLSEKGGKILFVGTKKQARGAVKEYAEKCGMYYVSERWLGGLLT
                     NYKTVSQSIKRLKSLQRMRDNDDWDAETKKERLELQRELEKKEKFLSGIADMDKLPDA
                     LFVVDPKREQIAVNEAHKLNIPIFAVVDTNCNPEEVDYPIPGNDDAIRAIALFLRVMV
                     DAIEEGRSGGMSAVEDDSDMDDDNAEASSSDSEESEVAEVSVEDTKE"
     gene            complement(9329..9565)
                     /locus_tag="P5123_03210"
     CDS             complement(9329..9565)
                     /locus_tag="P5123_03210"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRX15299.1"
                     /translation="MRFAKENTFYLILFIIVGGLLGGALGSLLAEIHPALVFLKKNLT
                     GFIGVNLDFIRLEVSLSLSSLLGIVSGIVIFAKA"
     gene            complement(9615..10841)
                     /locus_tag="P5123_03215"
     CDS             complement(9615..10841)
                     /locus_tag="P5123_03215"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRX15300.1"
                     /translation="MAIDKGSLDDFKFLNSVSEDFALSAQKVNDSSLQRKLHTENAIV
                     SLEDAERAGGMLLNELKIGSRESILLRSRHDAILNICRILKMNLDRQKILIDKLFTVK
                     PKNGFTVNHHYLDELDRLRKLLTQSLSKAFGILTEIVATGNEMILLDKLIQTSKKYQK
                     KGIIRLKKLTFTALKDAETAIEGSKLNHDRGAELINEIKRIPDYVEDKNTFALRAIVD
                     MANEGAELALHVNSSSKSQLEFAEKVNTVTGQLHDESLHIRKLVSDKHSLFTKNNELI
                     SQVAVIVAVEFFEYLSAMDIARHLKENDDLYDPEVIEAIYDLTAFVENACEDVESVAS
                     LNYDMTEAVAVNAKIEEKTVELTQKETGCFDMTRHQVMLMTEKTRFPIDGSARNIENG
                     RKVSDYVQRLLDSLTR"
     gene            complement(10849..11211)
                     /locus_tag="P5123_03220"
     CDS             complement(10849..11211)
                     /locus_tag="P5123_03220"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRX15301.1"
                     /translation="MKRDLSVGSLHIELLRESPEQLRMVWRGRSEDRDPADYLNPFLI
                     SSIEVLSDELLVCDFRELEYINSSTIPPILRMIKKLHIDGKSARIVYDSNSSWQTASF
                     TAFRIITDTMNLIEISGE"
     gene            complement(11201..11692)
                     /locus_tag="P5123_03225"
     CDS             complement(11201..11692)
                     /locus_tag="P5123_03225"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HRX15302.1"
                     /translation="MSAVVSMSASMPPTWSLVKEIRKNSSEYLQKGSHSSVDFDAVIM
                     VVSELVENAVKYGVRASDGTCVDFSLSVHGSRVKITVKNGIEKLEDYESVIFALDAIH
                     GPRSSEELYRSRLLQIYDSPIDEKGRLGLYRIAYEGGFDIACQLESDLIKLTAWRDFN
                     HEA"
     gene            complement(11951..13234)
                     /locus_tag="P5123_03230"
     CDS             complement(11951..13234)
                     /locus_tag="P5123_03230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020612025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="malic enzyme-like NAD(P)-binding protein"
                     /protein_id="HRX15303.1"
                     /translation="MNDLKNSALKYHSEPVPGKIEVVPSKPCKTASDLSLAYTPGVAK
                     PVLEIADNPESAYQYTSKGNLVAVVSNGSAILGLGNKGALASKPVMEGKGVLFKRFAD
                     IDVFDIELDTADPEKIIETVKMMEPTFGGINLEDIKAPECFIIEESLKKLCSIPVFHD
                     DQHGTAIISTAGLINALEIAKKNEGEIKVVFNGAGAAGVACAKLFLSFGVLRTNLIMC
                     DSKGVIYKGRKEGMTPEKEYFAVETDSRTLADALKGADVFVGVSVKDCVTPEMLKSMN
                     STPVVFAMANPDPEISYEDAISARDDVIMATGRSDYPNQINNVLGFPFIFRGALDVRA
                     SDITEGMKIAAARALADLAKEPVPDSISSAYDGKKFSFGSEYIVPKPFDPRVIEWVAV
                     AVAKAACEEKIAAKPISDWNAYSAQLRKRMEKYWK"
     gene            complement(13443..14396)
                     /locus_tag="P5123_03235"
     CDS             complement(13443..14396)
                     /locus_tag="P5123_03235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014455016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase family protein"
                     /protein_id="HRX15304.1"
                     /translation="MNYRRLGRSGLKVSEIAYGSWLTFANQVELDLAEKIIDKAVELG
                     INYIDTADVYAIGAAETLLGKILPRLNRRQLVIATKAFWPMSDSPTDQGLSRKHVFDS
                     LYGSLDRLNISYVDIFYCHRYDEETPLEETVEAIDDLIRQGKALYWGTSEWSAEQITA
                     AFNICQSRGFHTPIVNQPNYSLIVRGIEKQILPTCEALGMGTANFSPLGQGVLTGKYS
                     GGKIPAGSRGSDDSLNMYMKDSIANMDLLERVDKLKDIASEYEATIAQIAIAWILRNP
                     GISSVIAGASSIDQLESNVKASGIKLSEKHILQINALFPAE"
     gene            complement(14516..16945)
                     /locus_tag="P5123_03240"
     CDS             complement(14516..16945)
                     /locus_tag="P5123_03240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008825357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 3 C-terminal
                     domain-containing protein"
                     /protein_id="HRX15305.1"
                     /translation="MNGEESFNELLKNMSLEEKASLMSGSSFWLTEGVKRLGIPPIRV
                     ADGPHGLRKQPEGGDHLGIGGSIPSTCFPTAVTTASSWDVSLLHRIGEALGEECLQEK
                     VSVVLGPGVNIKRSPLCGRNFEYFSEDPLLAGEMSAAFINGVQSKGVGTSLKHFAANN
                     QEYFRFIIDTIVDDRALREIYLRPFEIAVKKSQPWTLMCSYNRINGKYSSDNKWLLTD
                     VLKNEWKHEGIVVTDWGACNNRVEGIKAGLELEMPGSGGINDRMIVKAVRDGKLDEAD
                     LDRCVLRMLDFINKASGVLEQHSSYDVNAHHDLAREAAEESMVMLKNESVLPLDKEAN
                     FCVIGDMAVKYRYQGAGSSQIVPTRLSNPFKELCLVCGREVAYSKGYDSSTDKTDRKL
                     IDQALAVSDKTETVIVFAGLPNIFESEGFDRDHMRLPDNQNELIEELIRAGKKVIVVL
                     SNGAPVELPWLNECHGLIEMYLGGQAGSEAIAKIIFGEVNPSGKLAETFPLKLEDTPC
                     YSYFPGNPQQVEYRESLYVGYRYYNSMDKPVAFPFGFGLSYTQFEYSDIKLSSSEIGD
                     DQNLSVVCNIKNIGNIAGKEVVQLYINDLESSFFRPAMELQGFTKVFLNPGESKQIEF
                     ELARDNFAFFDTATKTRQVEPGLFTVMIGASSRDIRLKADVNVVSGYKPVVDESLRSF
                     MDPYYRSEKPRGVFEVNDAAFEALLGNTIPPVQKTRRPFHRNSALGHIRETWMGRRLW
                     KIIAREFMNQMSVSDDETVNLMARRMLDEMPLRALVLLSGGVFTDKKLKACLDLLNGR
                     LVRGLLGMIRN"
     gene            complement(16945..17589)
                     /locus_tag="P5123_03245"
     CDS             complement(16945..17589)
                     /locus_tag="P5123_03245"
                     /inference="COORDINATES: protein motif:HMM:NF012655.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HRX15306.1"
                     /translation="MPVKRMIYQTPETRERILAMAQKLFLESGLFEVQMTDVARQTGI
                     SRNSLYRYFRDKTDLAFTLMEHRLKELVSNFEQLRDSLQGENYRAKIRDYFLKSWLNP
                     DYKETLLFLAEFDAYFSGVRQVLDQKERLQKTFGVSKDDLLTELVRKGQQEGSVRKDV
                     DAHFLMVTIVNGLRGLHQRLQLREESLLEVQGMESKHMMSHFLDIVITGIKGDR"
     gene            17858..18355
                     /locus_tag="P5123_03250"
     CDS             17858..18355
                     /locus_tag="P5123_03250"
                     /inference="COORDINATES: protein motif:HMM:NF025070.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis protein CheX"
                     /protein_id="HRX15307.1"
                     /translation="MKTPDEVKDLACQYFEKASDSIAFIFKSLLGLSVEPKRLYAKRL
                     NSLPNDIIINIDYRSGETEGFISFFFSLKIAMHICEKLVPSVDTTSFSEDHLDILGEM
                     GNMISGNAISKFQLINQDMRIQTPYHSGYFNILAAEKTHWIFSSNYECDLGKVGILLA
                     QKKDI"
     gene            complement(18383..>18836)
                     /locus_tag="P5123_03255"
     CDS             complement(18383..>18836)
                     /locus_tag="P5123_03255"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRX15308.1"
                     /translation="ADRLYPVLWIVVTDVSNRGKLQKLENELFQFSQPNLGRLPGFTE
                     DYALLLSRPGINELDIEKGKNNYLKEAAFLLHKIADFCDYLVRNPDANFEHPVNFSRG
                     YESADKKKKLTEEFGALTGFGAIFKIRHYCEDLISDFRLGAFRPANSR"
BASE COUNT         5638 a         4378 c         3294 g         5526 t
ORIGIN      
        1 gctcttgttt tcggcataaa aggctcaacc gcgttaaaat cggcacttac tctaggtatc
       61 gcttttattg gtatttttat gacctttgat ttttttgtaa aaataatcaa tccggtcatg
      121 caagcaatta tcgaaagatc aggacttaac ctgcccgtac tcgatacagg atggccgcct
      181 cttgccggga taacctggtc atattcactt gctccactga ttttattcat attgatagta
      241 atcaatatta ttcttcttgt aggccggtta accaagactg taaatatcga tatatggaat
      301 tactggcatg taattttttt ggcaacgctg atacaccata caacagaaaa tccgtggctg
      361 gcaataataa tcgcttccgc agcgttcata cttgtgctta aacttgcgga atggagtgct
      421 cctctggtta acaaattaac aggcatgaac ggcatctgca ttcctcatct ttcgggtata
      481 gttcattatc caatagcagt tgtaataaac cttctacttg atcgcatacc cggtttaaga
      541 tcggttaaag ccaatcctga acatcttaaa aagaagctgg ggcttttggg agaaccgatg
      601 attattgggc ttataattgg aattctgctt ggtcttgcag gcggttacga atttagggat
      661 attttaaacc tctctttcgg ttttgcggct gttgtcttta tactgcccaa aatgggtgaa
      721 attctgggat catctctgat accaatatca gaagggatga agctttttat tacccgtaaa
      781 ttttctcatc tcggagcaac ttttataggc cttgatgtcg cagtgctttt tgctgtacca
      841 gcagtacttg taacagctct tctgttgatg cctgtatcta taattcttgc aatattttta
      901 ccgggcatta atttcatacc aattggagac ctgacaaacc ttctggttcc tgtggcattt
      961 attgctgtgg caacaaaggg caatattata aaatcattta taatcggcat acctgttgtt
     1021 ataataaatc tctattatgc ttcaatgttc gcccctatag tgaccgatat ggcacgagta
     1081 tcaaattaca aaatagaggg gtatgacggt gtttttacaa gttttcttga cgcgggaaat
     1141 ccacttagaa gctggatcgt tcttcttctt gaaaaagaca tcctaagcat agcgcttgtg
     1201 cctgttgtta ttttaatggt ctttattacc tggaaatctt catcatccca aaagataaaa
     1261 aacaaatagt aacagcatgt cagtactaat gacagctata ataaatatat cagcaaaact
     1321 tatttattaa ctaaaagact ccgtttgtta gcggagtctt tttatgttta agagtttatg
     1381 gttacaaggg aactccaaaa aattgctatg ttccaatagg taatatgttt acaaaagtaa
     1441 tttataaatt tcaccataat atatacacaa taacttaaaa tacaagtttt gaaatatcgt
     1501 taaaaaggac aagaactccc aagcctatca acagaaaaaa gccaaaagcc tgcacctttt
     1561 ccattattcc agcaggtagc ggtttacccc ttaccgcctc aatcaaataa aatacaatgt
     1621 ggctcccgtc cacggcgggt attggcagca agttcataat catcagaatt atggatattc
     1681 gtgccataaa aagcaggtac tcacgtaccc cacggtagcg cattgtgtca cctgccaact
     1741 tagctatttg tacaggccca gacacattat ccttggcgct tatcttgcca gtaaaaagca
     1801 tcttcatacc ttgaagattt tgtacaataa aatcgtaagg gtcggtcagt gatttcttaa
     1861 acgctccggc taatccatgc tttacaggaa tcatttctgg tgcagcgaat gtggctacac
     1921 cgataaaacc actttttcgg tatttaacaa ccgcttcgta agagccgccc tttgtatcta
     1981 taacagcctt gccaccctcc agactctcaa taatagatag aaaatcatca aagtcggtaa
     2041 catttttacc gttcacagac accgtcccct tttttatagc cgttttaatc gtatccagat
     2101 cagaaacatt gatatcaaac gactcatcag gaaaaatagt actatgaaac tctgttaaaa
     2161 ccagctgctc ccgaaatctt ggagtaattt caatatccat attttcaccg gagcgttcga
     2221 tctgcaatac cagcggtacc tcgccgttat tttgcaatat ttcggaaaaa tgaccagaac
     2281 ctttcaacga ctctccgttt attgccttaa cccgatcata aacctgcaaa ccggccttct
     2341 gcgccacatc ggaaggttca agtcctgtaa tcaaaacatc ggttccataa ggcataatac
     2401 ctattgagta gtaccctcca ttgctatact gaaccggagt aacttttaaa tttatttttt
     2461 tcccatcacg tgcaacagta aaattcagcg gtttaccgtc agaaaaaacc acagccgact
     2521 gaatttccat aaaagaggat atctctttat catcaattgc gactacagta tcgccacttt
     2581 tcagaccagc cttttgagcc ggcaattcca tggagctgct ttcgggtatc attattctgt
     2641 ttgtctctac ttcataaccg gcaagattaa gagcaaaaaa aagtataatt ccgaaaaaca
     2701 ggttaaaaag aggccccatg accacagtta caatacgtcg ccagggagaa gcagtaaaaa
     2761 attcaaattt tttgccctct aaagttgcac caggaacatc accgtaaaaa gcgcaatatc
     2821 caccaaaagg tattaaggtt atctgatatg tggtatcacc ccactttttt ttaatgaaac
     2881 cctttccgta tcccaacgaa aaaacacgag ccttgatacc aaccattttg ccacctagca
     2941 gatgccctaa ctcatgaaca aagatagaac aacccaaaag cagtccagcc catagtatat
     3001 aaacaaaaaa atccggattc atcgcaaaac ctcatattaa tatattattt tacacaaata
     3061 attttaaaag ttcatttctt caaaatagtc tttttcggag tactttattc cattttatca
     3121 atatactctc tggccgtttc tcttgccaac ctgtcagcat caagaacaga ttgcacgtcg
     3181 cttgccggca aattgcaaag cgaatccact gcgtgcagtg ttacttcagt gatacttgaa
     3241 aatcctatac ggtgagataa aaacgaatgt acggcaatct cgttcgcggc atttaaaaca
     3301 acggtggctg ccccgccagc tcgtccggca ttgtaacaaa gatccaacgc aggataacgt
     3361 ctcttgtcta gctcgcgaaa ggttaaagat ccagccttaa caagatctag tttttcaaaa
     3421 ttattgggtc tgattcttgg atagagcatg gcatacgaaa tagggaaagc catatccgct
     3481 atacctaaat gcgcatgcaa agcaccacag gtagtctgaa caagcgagtg tatcagactt
     3541 tctggatgca ccagcacttt tatgcgttca tacggtaaag aaaaaaggtg atgcgcttcc
     3601 attacctcaa accccttttt catcagggta gcggaatcaa ttgttatctt gcttcccata
     3661 ttccaggtgg ggtgagcaag ggcttcttcg ggagttacat gggcgagttt ttctaaagga
     3721 taatctctca gacttccacc ggatgctgtg agaataacag aatcaatatg ctctggctcc
     3781 atgcaatcta aaagcgcgaa aagagcgccg tgttcactgt cgactggaat tatttcggtt
     3841 tcgcaactcg cggcttgttc gaaaaaaaga tcaccggcca taaccagagt ctctttattg
     3901 gcaagcgcta tccgcttaca gcttttcaaa gcataaagcg agggcaaaaa acccgcggcg
     3961 ccaactattg ccgaaacaca aatatcggca ccctctgata caacatcaag tgccgcctca
     4021 tcacctgtaa aaaatctggt ctttgggaaa catattttta acgattcaat ctgagcatca
     4081 tcacgaggtg atgttatacc acaaaaacga ggtttgtacc gctcaagttg ttcgccaaga
     4141 gtttgcacat tacaattaca aacgagccct tttaccgaaa agagatctgg atactcatca
     4201 attactctaa gagtgctgca acctatcgag cctgtagatc cgagaattat tacatccgaa
     4261 ggcatttaca ctccctttag tttaaggtag tagtaaaaca caggaaaaac aaatatcaaa
     4321 gcatcaaaga catcccacat tccgccatgc ccaggaataa tccttcccga gtccttgatc
     4381 tcggcatctc ttttaatagc tgattcaacc agatctccaa tattgcccag aagagacaga
     4441 agcataccca atagtatagc ttcaggcagg gaaaaaagat gtcggtcaaa aaagatctgg
     4501 aatgcttcgt tagcgataat tatgacaacc gtgctaaaca gcatacccga aaaatagccc
     4561 tcccaagact tgttgggcga aacgggaaaa ccggttttat gtgtaccaaa gaaaacccca
     4621 ccgaaatagg caaaggtatc gttaaacatg ataacaagat gcaaaataaa aatgtagtat
     4681 attccgtcgg gcagcgcttt cattaaaatt atatgtgaaa agggaagaac aataaatatg
     4741 ataccaaaca tggttgtagc aagagaaaat ataccgtcgg acaaaggcct tataacaata
     4801 tgcaacgcgc taattataac acctaaaaac acaaatattg ccagaacaat gcgcacatca
     4861 aaaattgcga gatacctctc gaatccataa acacggccaa aactgaacat atagataatc
     4921 acgtttataa caaagccagc ggccaaaccg ggaataataa aaggagtccc tccccccttt
     4981 gaagaggcaa tctggtaaaa ttcataaagg catgaaagag taactgcaag agaaattagc
     5041 aatattccaa gagtatagta ctggtcaaaa aatattagaa aaaaatagac cggtaacgca
     5101 attaaggcac ttattattct ttgaaatgta tcccatgtac gtgaagtcat aatcctccaa
     5161 accttctatt ccttttttga aattcgatta ccgctctgta caaatgagac ggtttaaaat
     5221 cgggccaaag tacctttaag aaaataaatt cggcataagc gcattgccat agcagataat
     5281 tactaattct acactctccc gaggttctaa ttaacaggtc aacatcaggc aaatcggaaa
     5341 cataaaggtt ttctgccatt tttttttgag taaacttttc tccaggctta gcttttgccg
     5401 accagttgtt aactgcgtca acaatctcct gtcgtgatcc gtaattaagg caaaaattca
     5461 ttttaagccc tttgtttccg gcagtctgct taaccgcttt atcaatcacc gaacagaccc
     5521 gagccggaag tctctttttt gacccggaat ggtaaacgcg aatacccttt tgatgcataa
     5581 ccggtagttt ctcggcaaag aaagagcaaa gcagttccca caaggcatta atttcagtag
     5641 aaggtcttga ccagttctca gtagaaaacg cgtaaagcga aacacaccct accgacaaat
     5701 ccatggctgc gtccattaaa gtctcgatta ccaaggcccc ttttcgatga ccatcggccc
     5761 taggcaaacc acgttttttt gcccacctac cgttaccatc cataattact gctatatgac
     5821 ggggaaccgc ggatttgtta acagagtcct ttaaagaggg cattatatgc ttaagatttc
     5881 cttctctttg ttatctgtca gtttttgaac catttcggta tatttatcag taagcttctg
     5941 aatattatcc tgttctttct ttgaatcatc ttcagatatg cttttatctt tttccagctc
     6001 tttaaccata tcgtttccgt cacggcgaac attacgtatt gcaactcggc aatcttcagc
     6061 cttattcttg gcctgtttta ccagttcttt tcgacgctct tcggtcaatt ccggaatctg
     6121 aatacgaata acattaccat catttgaagg attcagattc aagtcggctt ttaaaattgc
     6181 cttctcaatg gcacctatat ttcctttatc ccatggttga accattatca gacgagcctc
     6241 cggacaagat acagtcgcga cctgattaag tggcattgga gacccataag cgtctattga
     6301 aaccgaatcc agaatagatg ggttagcacg tccagttcta atagcaacaa actccttctc
     6361 aagagcctta acgcttttac tcattctcga ttcgacatct tttagtatat cgttaaccat
     6421 aaataacctc cgaaaacctt tttattgcgg atttttaata cagcaaccaa attaaagtat
     6481 atctgtacca ccagcatatc cgtttaacgc atccttaata cagttatcac gaaacagatt
     6541 aaatacacgt attggcagca tgttctccat gcacagcgat attgctgtta agtccattac
     6601 tctcaagtta ttctgaatca catcaatata gcttattttg tcaatttttt ttgcgtcaga
     6661 atgtttggca ggatctttat cataaactcc gtctaccttt gttgctttca tcataagatc
     6721 gcatttaagc tcgacagctc ttaacgttgc tgctgtatct gttgtaaagt agggattacc
     6781 tgttccaccg gctacaatca caatcttgtt atcggcaaga agcgcagaag ctttgcgtac
     6841 cgtatagtaa tcggccactg tttccatggc aaaagatgtc ataacaaccg cattacgtcc
     6901 ggcagaagag atgctatcag ctaaagccaa tgaattaata acagttgcca gcatccccat
     6961 gtgatcacct gttacacgat ccataccaag gctttctccc aaaccgcccc taaaaatatt
     7021 gcccccgcca ataacgaggg caatctgaac accgctttca gcggcaagga cgacttcaga
     7081 ggcaaccttt tttacagttt caggatcaag cccagccgaa gcagaaccgg caagaacctc
     7141 accgctaagc ttgaccagaa cccgtttata agggtttgct tcactcatca tcaaccgcca
     7201 atttgaacac ggataaattt ttctattttt attgggcttc cagccttagt actgtactct
     7261 ttaacaacat cggaaacctt tttcgcgcta tcttttataa aaacctggtc aaccagacaa
     7321 acatccgaat agaattttga aagtttacct tcaactatct tgtctataat attttccggt
     7381 tttcccgaat ttttcatctg ctctttgtaa atttcacgtt ccttatcttt aacagtctca
     7441 ggcacttcgt ctgaagttac atattgtgga aaagcagcag cggcctgcat agcaagttca
     7501 cgaccaagtt cgcacaaaat agccgagtca gaagcatctg aagcaagagc aaccaacaca
     7561 ccaatcttac cgttactatg aatgtatgaa gctattgctc ccttatcgat ttcgatagta
     7621 tcgaatgaac ctatgcccat attttcgcct gttgtagcaa tagcaccctt gatagtctca
     7681 tcaagcgctg ctggtatttc agctccgctt tttagagagt tgtcaaaaat ctcggatgtt
     7741 atcttgttac ccaaattatg aaactcttca tttttagcaa caaaatcagt ttcgcagtta
     7801 agctcaacaa tagaaccttt tttgccatca tcagaaagtt tgacgaatac aagaccctct
     7861 tttgcttcac gtccggctct tttatttgcc gcagcaaggc ctttttttcg taaaaattcg
     7921 gcggcctttt ccatatctcc atcacattcg gtaagcgctt ttttacagtc aagcatacca
     7981 gcctgtgttc tttctctcag ttcttttatc attgatgcag ttatttcagc cataactacc
     8041 tcctctatcc ttaatataac acttttataa cggtgtttat gatcttacac ggcacctgaa
     8101 attcaggtac cgtgcaaatc aatagaagat ccgctaatct tctttcagct ctactctttg
     8161 gtatcttcta cagaaacctc agcgacttct gattcttcag aatcagaaga tgaagcctcc
     8221 gcattatcat catccatatc agagtcgtct tcaacagctg acattccacc cgaacgcccc
     8281 tcttcgatgg cgtctaccat gacacgcaag aaaagcgcaa tagcgcgtat tgcgtcatca
     8341 ttaccaggga taggataatc cacctcttcg ggattacagt ttgtatcaac aacagcaaaa
     8401 attggaatat taagcttatg agcctcgttt acggcaattt gttcgcgctt tggatcgaca
     8461 acaaacaacg cgtctggaag cttatccata tctgcgatac ctgataagaa tttctctttc
     8521 ttttcaagtt cacgctgaag ctcaagacgt tcttttttgg tctcggcatc ccagtcatca
     8581 ttatcacgca ttctttgaag actttttaga cgctttatcg attgagaaac agtcttgtag
     8641 ttggtcaaaa gacctccaag ccaacgctcg gaaacgtagt acatgccaca tttttcggca
     8701 tattctttta ctgcaccgcg tgcctgcttt tttgttccta cgaataaaat ttttccgcct
     8761 ttttcagaca gatctttcat aatggaatat gtagctttta gccgctgcat tgtcttttga
     8821 agatcaataa tatgaattcc attacgggat gtgaaaataa actgctgcat cttggggttc
     8881 cagcgacgag tctgatgacc gaagtgtaca cctgattcca agagctcttt cattgttata
     8941 acagccaatg aaaaccccct ttttaaacct gattttattt tttacggata taccgcctgt
     9001 ctggacacaa ttcagatacg atctgaatat aattctgatg caatctgaat ataattctga
     9061 tgcaatctga atataattct gatgcaatct gaatataacc caaacataat acaaagaaac
     9121 gcctggggtt aactaacctc aaaccgtact tcgtacagat aacgcgccgc atagctttct
     9181 tttttaacca atcaagatca aaaaacgaat tgagttcaac cggaaataaa taccgattta
     9241 tacgtcttta ataataatgg aaacccttac aagaatatcc gattacaata aagacgaaat
     9301 agctgcgggc gcagccggcc tttcgacctt acgctttcgc gaaaatcact attcctgaaa
     9361 caattccaag aagtgacgag aggcttaaag atacctctaa acggataaaa tcgagattaa
     9421 ccccgataaa ccctgtaaga ttttttttga gaaaaacaag tgccggatga atttccgcca
     9481 gaagcgaacc taaagcgcct cccagaagac ctccgacaat gataaacaat ataagataaa
     9541 aagtgttttc ctttgcaaat ctcacagcaa cacgattatc gaatggctgt ttttgtcaag
     9601 tacaaagatt caatttaccg agtcaaactg tctaacagac gttgaacata gtcactgact
     9661 ttgcgtccat tttcgatatt tcgagcggat ccatcaatag gaaagcgggt cttttcggtc
     9721 ataagcatga cctgatgacg cgtcatatca aaacaaccgg tctctttctg tgttagttcg
     9781 acagtttttt cttcaatttt cgcattaaca gctacagctt ctgtcatatc ataattgaga
     9841 ctggcaacag actcaacatc ctcacacgcg ttttctacaa aagccgtaag atcatatatg
     9901 gcctcaataa cctcgggatc gtaaagatca tcgttctctt taaggtgacg cgcaatatcc
     9961 attgccgaaa ggtattcaaa gaactctaca gcaacaatta ccgctacctg tgaaattagt
    10021 tcgttatttt tagtaaacag ggagtgctta tcggaaacca atttacgtat atgaagtgac
    10081 tcgtcatgaa gctgaccggt tacagtattg accttttcgg caaactcgag ctgcgatttt
    10141 gaactactgt tcacatgaag agcaagttcg gcaccctcgt tagccatatc aactatagca
    10201 cgaagggcaa atgtgttctt gtcttctaca tagtccggga ttctcttgat ctcgtttatc
    10261 agttcggcac cgcgatcatg gttgagtttc gatccctcga tggcggtttc ggcatcctta
    10321 agagcggtaa atgtaagctt ctttaaacga attatccctt ttttttgata tttttttgac
    10381 gtctgaataa gtttatcaag gagtatcatc tcgttaccag ttgcaacgat ttcggttaat
    10441 ataccaaaag cctttgataa cgactgtgtt aacagttttc ttagcctatc tagttcatca
    10501 agatagtgat gatttacagt aaagccattc ttgggcttaa cagtaaaaag cttatctatt
    10561 aaaatctttt gacgatccaa attcattttt aaaatgcgac aaatattcaa aatggcatcg
    10621 tgacgacttc tcagaagaat actctccctg ctgccaattt ttaactcatt caacagcatg
    10681 ccaccggctc tttcggcatc ttcgagtgaa acaatagcat tttccgtatg aagcttacgt
    10741 tgcagtgaag aatcattaac cttttgggca ctcaacgcaa aatcttcgct aacggagttc
    10801 aaaaacttaa aatcatccag tgacccctta tctattgcca taatctgcct actctcccga
    10861 aatctcgatc agattcatcg tatcagtaat aatacgaaac gcagtaaaag atgccgtttg
    10921 ccatgatgaa ttgctgtcgt atactattct agcagacttc ccatcaatat gcaacttttt
    10981 tatcatacga agtataggcg gtatagtaga agagttgata tactcaagtt ctctgaaatc
    11041 acaaacgagc aactcatcgg aaagaacctc aattgatgag ataagaaaag gattcagata
    11101 atcggcagga tctctatcct cgctgcggcc tcgccatacc attcgtaact gttccggact
    11161 ttcgcgcaaa agttctatat gcagcgaacc aaccgacaaa tcacgcttca tggttaaagt
    11221 ctctccaggc agtcagcttt atcaagtcgc tttccagttg gcaggcaata tcaaaccccc
    11281 cctcgtaagc tatacggtag agccccaaac gccccttttc atcaattgga gaatcatata
    11341 tttgtagaag acgcgatcta tatagttctt cactggatct gggtccatga attgcatcaa
    11401 gggcaaaaat tacagattca taatcttcaa gtttttcaat accgtttttt acagttattt
    11461 ttacacggga accgtgaaca ctcaggctaa aatcgacaca cgtaccgtca gaagccctaa
    11521 caccatattt tacggcattt tcaactagct ctgaaacaac cataatcacg gcatcaaaat
    11581 caacagacga atgtgagccc ttttgaagat attcagagct atttttcctg atctctttta
    11641 caagcgacca ggttggcggc atggaagcac tcatggagac cacagcactc attcggtatc
    11701 ctccggtgat aagcaatcac ggaaaaaacc ctcagaacag acaggtttat atcttttgta
    11761 atcaaaaact aaagcagata atttacaaag gatgggtttt aacaaactct aaacctagct
    11821 gtttatgtaa aacatcaccc attcacaaac acctgttgac tataatatac aaagaaatta
    11881 ccgaaattca atttattgcc agataattat caacaaaatc gcatgttttt tgatactaaa
    11941 gtaccagtaa ttatttccaa tacttctcca ttcttttacg caattgcgcg gaataggcat
    12001 tccaatcact gatcggtttt gcggcgattt tttcttcaca ggcagccttg gcaaccgcaa
    12061 cagcaaccca ttctattaca cgcggatcaa aaggttttgg aacaatatac tctgatccaa
    12121 aactgaactt ttttccatca taagcactgc taatactgtc aggaaccggc tcttttgcca
    12181 aatcggcaag tgcccgggcg gcagcaatct tcataccttc agtgatgtcg gaagcacgaa
    12241 catctaaagc tccacgaaaa ataaagggaa accctaacac attatttatc tgattaggat
    12301 agtcactgcg accggtagcc ataatgacat catcacgggc gcttatcgcg tcttcataag
    12361 atatttccgg atcaggatta gccattgcaa ataccacggg tgtcgaattc atagacttaa
    12421 gcatctcggg tgttacacaa tctttgactg aaacacccac aaagacatca gcacctttta
    12481 aagcatccgc caaggtgcga gaatcggttt caaccgcgaa atactctttt tcaggtgtca
    12541 taccctcttt acgcccctta tagataaccc ccttgctgtc acacattatc aagttagtac
    12601 gaagaacccc aaaactcaga aatagtttcg cgcaagcgac tcccgccgcg cctgcaccgt
    12661 taaaaacaac ctttatttcg ccctcatttt ttttagcaat ttccaaggca tttatcaaac
    12721 ccgccgtaga aattattgcc gtaccatgtt gatcatcgtg aaaaacaggt atggaacaga
    12781 gcttttttag gctctcttct ataataaaac attctggtgc ctttatatcc tcgagattta
    12841 ttccgccaaa tgttggctcc atcattttaa cagtctcgat tattttttct ggatctgccg
    12901 tatcaagctc gatatcaaaa acatcaatat cggcaaatct tttgaaaagt acacccttgc
    12961 cttccataac cggcttagaa gcaagagccc ccttgttgcc cagccccagt atggcacttc
    13021 cgttagatac aaccgctaca agattgcctt ttgaagtata ttgatatgcc gattccggat
    13081 tatcagcaat ttctaaaacg ggttttgcga cacccggagt atatgccaat gacagatcag
    13141 acgcagtctt gcagggcttg cttggaacaa cctctatttt tcccggcaca ggctcgctgt
    13201 gatattttaa cgcgctattt tttaaatcat tcatttttaa acctcgatca tattacgata
    13261 cggctaagaa accggtgaaa cagaactcta atttttttat gaggtcatac ttattgcagt
    13321 agcttttaaa tcaagatttt atttaaacga aattttttat ctctttatcg ataaaaaaac
    13381 agggtatgaa taaccctgct ttttaactta gattatttat aatcacataa gcaccaaaga
    13441 tattactcgg cgggaaaaag cgcgtttatt tgcaaaatat gtttctctga taattttatg
    13501 cctgatgcct ttacgttgct ttcaagctga tcaattgaag atgctcctgc aatcacggat
    13561 gatatacccg ggtttcgcaa tatccaggca atcgcaatct gtgcaattgt cgcctcatat
    13621 tcacttgcta tatctttaag cttatccaca cgctcaagca gatccatatt ggcaatagaa
    13681 tctttcatat acatgtttaa cgaatcatca gaaccgcggc tacccgctgg aattttgccc
    13741 ccactatact taccagttaa gacaccctgc cccaatggag aaaaatttgc tgttcccatt
    13801 ccaagagcct cgcatgttgg caaaatctgt ttttcgattc cgcgaacaat caggctatag
    13861 ttgggttgat tgacaatggg agtgtgaaac cctcggcttt gacagatatt aaaagcagcg
    13921 gttatctgtt cggcactcca ctcactcgtt ccccaatata acgccttgcc ctgacgtata
    13981 agatcgtcta ttgcctctac agtttcttca agcggagtct cttcatcata ccggtgacag
    14041 taaaaaatat cgacataact gatattcagc ctgtcaaggc ttccataaag cgagtcaaaa
    14101 acatgcttac ggcttaatcc ttgatccgtt ggtgaatcac tcataggcca aaaggcctta
    14161 gtagcaatta caagttgacg gcgatttaaa cgtggtaata ttttaccaag cagtgtctct
    14221 gccgcaccga tggcataaac atccgccgtg tcgatatagt tgataccaag ttcaacagct
    14281 ttatctatta tcttttcagc cagatcgagc tctacctgat tagcaaatgt taaccatgat
    14341 ccataagcaa tttcactaac cttaagcccg cttcttccta atcttctgta gttcataaaa
    14401 atcttcctta taaaattgaa tcctaaaaaa aaccagcgaa ttccaagatt ttcttttcta
    14461 taaaaatacc ttagattctc ttactttaaa gcaaccgtca tagcaacctt tctgatcagt
    14521 ttctgatcat tcccagtaat cctcggacaa gccttccgtt aagcagatca agacacgcct
    14581 tcaacttttt gtctgtaaaa actcctccgc ttaaaagaac aagcgccctt aacggcatct
    14641 catcaagcat acggcgcgcc ataagattca cggtttcatc atccgaaacg ctcatctgat
    14701 tcataaattc acgcgcaatg attttccata accgtctacc catccacgtt tctcgaatat
    14761 gccctaaagc actgtttcga tgaaatggtc gtctcgtttt ttgaacaggt ggaatagtat
    14821 ttcctagtag agcctcaaaa gcagcatcgt tgacttcaaa tactcccctg ggtttttctg
    14881 acctataata gggatccata aatgacctaa gcgattcgtc cactaccggc ttgtatccgg
    14941 aaacaacatt gacatctgct tttaatctga tatcacgcga tgaagcaccg atcataaccg
    15001 taaaaagtcc tggctcgacc tgacgggttt tagtcgcggt atcaaaaaaa gcaaagttat
    15061 ctctggcaag ctcaaattca atttgtttag attcgccagg attaagaaat acttttgtaa
    15121 acccttgaag ttccatcgca ggacgaaaaa acgagctttc taaatcattg atatacaact
    15181 gtactacctc tttaccagca atattaccga tgtttttaat attacagact acagataaat
    15241 tttgatcatc acctatttcc gaagaggaga gcttaatatc agagtattca aactgcgtat
    15301 atgataatcc gaatccgaac ggaaaggcca ccggtttgtc catactgtta taataacggt
    15361 atcccacata gagactttct cgatattcaa cctgctgcgg atttcccgga aaataactat
    15421 aacaaggggt atcttccagt ttaagcggaa aagtctcggc cagttttcca ctgggattta
    15481 cttcaccaaa gataattttc gctatagcct ctgatccagc ctgtccacca aggtacattt
    15541 cgataaggcc atgacactca tttaaccatg gcagctccac aggcgctcca ttgcttagta
    15601 caactataac ttttttacca gcccggatca gttcttcaat aagctcattc tgattgtcag
    15661 gcaatctcat atgatcccgg tcaaaacctt cggactcaaa aatattgggt aatccggcaa
    15721 aaacaataac cgtttcggtt ttgtcactaa ccgcaagagc ctgatcaatc aattttctgt
    15781 ctgttttgtc agtcgaactg tcataaccct tagagtaggc gacctcacga ccacacacca
    15841 gacatagctc cttaaatgga ttgcttaaac gggtaggtac tatctgagag ctgcctgctc
    15901 cctgataacg gtatttaacg gccatatcac caataacgca aaagtttgcc tctttatcaa
    15961 gaggcagcac cgattcattt ttaagcatca ccattgactc ttcggcggct tctcgtgcca
    16021 aatcatgatg cgcgtttaca tcataactgc tgtgttgttc aagaactcct gatgccttgt
    16081 ttataaaatc taacattcga agcacacatc tgtcaagatc tgcctcgtca agttttccgt
    16141 cacgaaccgc ctttacaatc attctgtcat taattccgcc gcttcctggc atctccagtt
    16201 caagcccagc cttaattccc tctacgcgat tattacaggc accccaatcg gtaacaacta
    16261 tgccctcatg tttccactcg ttttttagaa catccgtcaa aagccactta ttatcacttg
    16321 agtatttacc atttattcga ttgtacgaac acatcaatgt ccagggctgc gattttttta
    16381 cagcaatctc gaaaggacgc agatatattt cgcgcaaagc ccgatcatca acgattgtat
    16441 ctattataaa gcggaaatac tcctggttat tagcagcaaa atgcttaagg cttgtaccaa
    16501 cacccttact ctgaacgcca ttgataaacg ccgcactcat ctcccctgcc agtaaaggat
    16561 cctcagaaaa atattcgaaa ttacgtccgc aaagcgggct tctttttata ttgactcctg
    16621 gccctaacac aacagaaact ttttcctgaa gacactcctc tcccagggct tcaccaatgc
    16681 ggtgcagaag agaaacatcc caagagcttg cagtggtaac tgcagtaggg aaacatgtcg
    16741 aaggtatgga tccaccgatt cccaaatggt ctcccccttc gggctgtttg cgcagaccat
    16801 gcggcccatc tgcaacacga atagggggta ttccaagccg cttgaccccc tctgtaagcc
    16861 agaaactgct tccgctcata agagaggcct tctcttccaa gctcatattc tttaaaagct
    16921 cattgaagct ctcttctcca ttcatcatcg gtctcctttt attcctgtaa taactatatc
    16981 aagaaaatga ctcatcatat gttttgattc cattccctga acctctagca gtgactcttc
    17041 gcgtaactga agacgctgat gcaaacctct aagtccgttt actatggtaa ccatcaagaa
    17101 atgggcatct acatcttttc gtacagaccc ctcttgctgc cctttgcgca caagctcggt
    17161 taacagatca tctttgctta caccgaatgt tttttgcaac ctctctttct gatcaagcac
    17221 ctgccttact ccagaaaaat aggcatcaaa ctcggcaaga aaaagaaggg tttctttata
    17281 atcagggttc aaccagcttt ttaaaaagta gtctcgtatt ttcgcgcgat aattttcacc
    17341 ctgcaagctg tcacgcagtt gttcaaaatt actaacaagc tcttttaagc gatgctccat
    17401 taatgtaaaa gcaagatcgg tcttgtctct gaaatatctg tacaatgaat ttcggcttat
    17461 acctgtttga cgcgcgacat ctgtcatctg tacttcaaat agcccagact ccaaaaacag
    17521 cttttgtgcc atagcaagta tgcgttcacg cgtttcgggt gtctggtaaa tcattcgttt
    17581 tacgggcata acgttcaccc tcccttatgt tacatgtgta atacatcgcc tttccctctt
    17641 atttgtcaag aaggaagtac caaaaatatc aatgtagggt tggcatcggc cttaagactg
    17701 ttttgtatgt cgatttctaa atgaacactg ttcaattaat tcttttattg acataatttt
    17761 cgaaaagcgg ataattagtt ttaaatacgc atgcacaata ctgataagta cattttcaaa
    17821 cacattgtac cataagttat taccccttgg agccataatg aaaacacctg acgaagttaa
    17881 agatcttgca tgccaatact ttgaaaaagc ctcggactcg attgccttta tttttaaatc
    17941 cctacttggt cttagtgttg aaccgaagcg cctttacgcg aaaagactca actctcttcc
    18001 taacgatata ataataaaca tcgattacag atcgggcgaa acagaaggct ttatctcgtt
    18061 tttcttttca ttaaagatag ccatgcatat ctgcgaaaaa ctggttcctt ccgttgatac
    18121 gacatctttt agtgaagatc atctggacat attaggtgaa atgggtaata tgatttccgg
    18181 aaacgcaata agcaaatttc aactaatcaa ccaggatatg cgcatacaaa cgccttacca
    18241 ttcgggatat tttaatattt tagcggccga aaagacccac tggatattct ccagcaatta
    18301 tgaatgtgat ttggggaagg tgggcatact gcttgcgcaa aaaaaggata tttaatattc
    18361 acttatcttg ttgcaagagc tgctatctgc tattagctgg cctaaaggcg cctagcctga
    18421 agtccgagat cagatcttca caataatggc gaatcttgaa tattgctcca aaacctgtta
    18481 aagcaccaaa ctcttcggta agtttctttt ttttatcggc actttcgtac ccacgggaga
    18541 aatttacggg atgttcaaaa ttggcatccg ggttacgtac aaggtaatca caaaaatcgg
    18601 caatcttgtg taataaaaag gctgcttcct ttaaataatt gtttttaccc ttttctatat
    18661 ccaattcatt aattccaggt cttgaaagca gaagtgcata atcctctgta aatcccggaa
    18721 gccttccaag atttggttga gaaaactgaa aaagctcgtt ttccagtttt tgaagcttac
    18781 ctctgtttga gacatctgtt actactatcc atagaaccgg gtaaagtctg tccgca
//
LOCUS       DAOTGZ010000027        18652 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_3064
            NODE_3572_length_18652_cov_4.851428, whole genome shotgun sequence.
ACCESSION   DAOTGZ010000027 DAOTGZ010000000
VERSION     DAOTGZ010000027.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868088
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 18652)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 18652)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:22:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,785
            CDSs (total)                      :: 2,751
            Genes (coding)                    :: 2,732
            CDSs (with protein)               :: 2,732
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 12 of 19
            Pseudo Genes (internal stop)      :: 5 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18652
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3572_length_18652_cov_4.851428"
                     /isolate="AMR_MDS_3064"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            607..1425
                     /locus_tag="P5123_03260"
     CDS             607..1425
                     /locus_tag="P5123_03260"
                     /inference="COORDINATES: protein motif:HMM:NF013250.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-alcohol phosphatidyltransferase family
                     protein"
                     /protein_id="HRX15309.1"
                     /translation="MKIEEYRKLCQKNENENYFFDKYVFRKISIFFTIFFTFTGVNAN
                     TVTFLSLLCSIGAAWFALFNTDTALSLVVILIFLYYILDHVDGELARYLINKGKMKPS
                     LVGAYFDQLVHKYSANLILFSFGYAVFTEYGYTWALFTGFICCIGLSGFPNLLNYKVI
                     LQKIITDPNIMQKAEYKELLDRCDQKGNSIKIIQKGLGLKKYKKIAGEILVFPGGVLL
                     CAIAALLDLVFKDIIYMGFGFNFRLLLIVGLTPVYLLKVVLDWRKWMKQLKNAG"
     gene            complement(1430..2626)
                     /locus_tag="P5123_03265"
     CDS             complement(1430..2626)
                     /locus_tag="P5123_03265"
                     /inference="COORDINATES: protein motif:HMM:NF016357.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0047355 - CDP-glycerol
                     glycerophosphotransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-glycerol glycerophosphotransferase family
                     protein"
                     /protein_id="HRX15310.1"
                     /translation="MVIDILLDILLIFLSLVAPKKKGSFLMGASRGDQFIGNPAYFYR
                     YLLDANHQNTYWITGCKELFASMESDGYPVLYKYSFKAFTWILRSEFLVIEQMPRDIL
                     YFGLSSIGCFNYVQTFHGIALKKIGLDAAREGKGIAGFNLFKIKIFDRFANMLKRDFK
                     KYFLYRPYKYIIATSQMTADICKSAFGSKRVHILGYPRNDIFYQSYPIPLKVLDLTKN
                     YRKVFTYVPTFRDNYTMVRPFSDKFYAQIEKEFKKRNWLLAIKKHPYDKNIVVPRNLE
                     YIKDCSDIVVDLQPLLLVTDVLITDYSSVTNDFCLTGRPVIFYTYDVEEYRNRCRSLY
                     LEPDEAYSGPFALDEDALGKLALSVERWSKEKKFKEQYKEMVNRLNTYKDGNSSERLL
                     RFLQDR"
     gene            3132..3755
                     /locus_tag="P5123_03270"
     CDS             3132..3755
                     /locus_tag="P5123_03270"
                     /inference="COORDINATES: protein motif:HMM:NF013921.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - transposition, DNA-mediated
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HRX15311.1"
                     /translation="MMRKPREIVDGSTYHVSAKINRGEFIFKDRADLKILFFKLVKRT
                     HGKYKFHVQGITVMDNHIHMTIKPSKGEKLGNIMNSILSTFARRYNVIINSHGHVWYD
                     RFKSKVIVTFTQYINTLRYICNNPLRAGMVNDPLSYKFCAVNALYTKEKSELVSIIQS
                     IIDFKNKELLNIYDDYIKEFDVNKAIEKDITIGFYPNNPGRPKKDKK"
     gene            complement(4301..5731)
                     /locus_tag="P5123_03275"
     CDS             complement(4301..5731)
                     /locus_tag="P5123_03275"
                     /EC_number="2.7.7.13"
                     /EC_number="5.3.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004287271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004476 - mannose-6-phosphate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mannose-1-phosphate
                     guanylyltransferase/mannose-6-phosphate isomerase"
                     /protein_id="HRX15312.1"
                     /translation="MVNIVLCGGSGTRLWPLSRKLYPKQFVPLFEGKSLFQKTVVRNS
                     SICDKFIFVTNRDLHFMTADQSEDSGVSSIKNMTRFILEPLGRNTAPAIALACMDCNK
                     DDVVLVTPSDHLIKDTENYLANAIEAEKLATEGYLVTFGIAPSYPETGFGYIEAGKKL
                     GDNDNYLIKAFKEKPDENTAKEYLQQGNYYWNSGMFIFKAKVFLDELKKYAPEIYEAS
                     RNAYNNSERRFEQDTDSLFVPYEKMAAIPADSIDYAVMEKSDKGAVIRSDFGWNDLGS
                     FDSLYEVLPKDNAGNTVDTNHVSIDSKNNLIIGCDRTISTIGISDSIIIDTDDALMIA
                     KRGDSQKVKEIVDILGDGGLHKTELTEVHRTVHRPWGTYTVLEESSNFKIKRIMVKPG
                     RRLSLQKHMHRSEHWVVVTGTATVEIEGKEQIVRPNESVYIKIGDSHRLTNNGMVPLN
                     IIETQVGQYVGEDDIIRVDDDYMRKM"
     gene            complement(5765..7093)
                     /locus_tag="P5123_03280"
     CDS             complement(5765..7093)
                     /locus_tag="P5123_03280"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010932849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose/GDP-mannose dehydrogenase family
                     protein"
                     /protein_id="HRX15313.1"
                     /translation="MKITVVGTGYVGLVTGACFADMGNDVWCIDIDENKIENLKKGII
                     PIYEPGLEEIVKRNYSEGRLNFSTSVSDGLEKSLFAFIAVGTPPGEDGSADLQYVLSV
                     ANDIGNNMKGYTIVINKSTVPVGTAEKVREKIKVVLAERDSSDIEFDVVSNPEFLKEG
                     DAVDDFMRPERVIVGCDNVRVAELMKELYSPFTRSEDRFYSMDIPSAELTKYAANCML
                     ATRISFMNEMSRICDATGANVEMIRKGIGSDSRIGRSFLYAGIGYGGSCFPKDVKALV
                     RTSKQLGMESKILNAVEEVNLEQRQIFAQKIVDHYGGAVKGKKFAVWGLAFKPQTDDM
                     REAPAVDIINYLYKLGAIFTAYDPIAVNNAKPLLPDSGIDYVNNNYDCLKDADALIIM
                     TEWHHFRRPDFERIKSLLRQPVIFDGRNQYKCSSMKEREFTYISVGRPEV"
     gene            complement(7316..8707)
                     /locus_tag="P5123_03285"
     CDS             complement(7316..8707)
                     /locus_tag="P5123_03285"
                     /inference="COORDINATES: protein motif:HMM:NF014740.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide biosynthesis protein"
                     /protein_id="HRX15314.1"
                     /translation="MNKGAVLIYGAGSAGQSIATELLESSITVAGFVDDDSKLIGTSI
                     NGIRVLGESKDIEKIVESDNIEQFIVAIPSANTEQINNALYRVLQYDRNINVKILPYV
                     TRFFDSPLINELEDSSYIKLIDRDEITCDTTLLSKEYCGKKILVTGAGGSIGSELCRM
                     LMRLNIDSLICLGHGEGSIYNLSQTMTGFNKKISVSYVIADVRDGSSIDSILKKFKPD
                     VVFHAAAHKHVPLMEFNIREACLNNIGGTSTLLNSSEKNEVKSFVFISTDKAVEPSSV
                     MGATKRAGEILTMSYNTKMKTSVVRFGNVLGSRGSVIPLFNNQVDRGGPVTITHPEMR
                     RYFMTIPEAALLVIHAAVLKSHPLYILNMGREYLIKEIAENIIKSKGLTPEIDIKIEY
                     TGLRPGEKLREKLSYDNEKKGETVNPNITGLHPEYCCNEESQLFIDNYLKEISKNNVV
                     IMDLIKELNIIIG"
     gene            complement(8709..9860)
                     /locus_tag="P5123_03290"
     CDS             complement(8709..9860)
                     /locus_tag="P5123_03290"
                     /EC_number="2.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012673735.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DegT/DnrJ/EryC1/StrS family aminotransferase"
                     /protein_id="HRX15315.1"
                     /translation="MEIPFHKVDITEEDIDAVVESMRSGWLTMGPRTFEFEKLFAAFL
                     EKDEAVAVSSCTAALHLALVAENIGPGDEVIIPITTFVATAEAVLYTGATPVFADIEK
                     DNHTIDTNHVRNLITENTRAIIPVHYAGNAADMDPIMSIAAEHNLVVIEDAAHAFPAT
                     YKGRALGTIGDYGCFSFYATKTITTGEGGMIVGANSRKLDRIRSLRLHGITRDAWNRY
                     GKGGSPVYDVTENGYKYNMTDTAAALGLSQLRRCGSSLQKRQQIAKIYDKYFSDNPYI
                     LPYEIENYVECAFHLYPLKINIDELKIDRHNFSKKLLEKGVSSSLHFIPFYEFSYYKD
                     KLDAHNYPGADWVYKREISLPIYPGLSDNEVNYVAKTVIKTAEDCVEGR"
     assembly_gap    9913..10012
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(10353..11606)
                     /locus_tag="P5123_03295"
     CDS             complement(10353..11606)
                     /locus_tag="P5123_03295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007085719.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mechanosensitive ion channel"
                     /protein_id="HRX15316.1"
                     /translation="MLVSYLNELLSQFNFDPAYLNMITYFVLAFIVFLLSFTANFITK
                     KIILKAASWYIKNNRFTWDNIMLERKVFSRLSHCVPAVIIYIFINFFPDYLQEPLNRG
                     VKVYIIFIALITLYALLDAVNDIYNSYSISKTRPIKSFLQVAKIFATVIAIVLMIAGI
                     MGKSPSLLLGGIGALSAVLMLVFKDSILGLVGGVQLSANDMVRVGDWIEMPGYGADGD
                     VIDISLNTVKVQNFDKTITTIPTYALVSNSFKNWRGMQESGGRRIMRSVHIDVSSIKF
                     CTDGMLDSYLKIFHLRDYIDCRQKEIEEYNKSFGADPSVPINGRRMTNIGVFRHYIAN
                     YLKNHPKIHDGMIQIVRQLPPSEKGLPLQLYVFTNDTNWVNYEEIQADIFDHILAAAS
                     WFDLKIFQNPTGNDFRFALNKNGTN"
     gene            complement(11668..13626)
                     /gene="recD"
                     /locus_tag="P5123_03300"
     CDS             complement(11668..13626)
                     /gene="recD"
                     /locus_tag="P5123_03300"
                     /EC_number="3.1.11.5"
                     /inference="COORDINATES: protein motif:HMM:TIGR01447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009338 - exodeoxyribonuclease V complex
                     [Evidence IEA];
                     GO_function: GO:0008854 - exodeoxyribonuclease V activity
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease V subunit alpha"
                     /protein_id="HRX15317.1"
                     /translation="MSKKNGTTIDNAKDIKLTSSTTFNNIKSKDDFYNRYSQLFIEEI
                     FCEADRLSSDYILEQEEFYDPLYHLILLALSRVTLNAHVCYQLGVSPFDDDNLYEYND
                     SVVNMTNELADQLVKFVSEDFFNKFKTVGTGSSVTPVVYFNGLLYFYKQFFQEKKCAL
                     LLLDKASQIKNDVSEEIDKVSKQIFAYNENNKEADWQYIAARAALKRNLTIITGGPGT
                     GKTTTVYKIYMLLAAIERMKGNSFTAVLTAPTGKAAARMFTAFSKARSVVTEDMSMEK
                     NLKKWLKDDDIEALTVHRLIGSARQHIDQHIEKLPYNLVIVDEASMMSLSLLYKLLLR
                     LRDDSILILLGDSRQLASVDAGAIMADICYRNNGKLYSNEFKKFAVNCGVNIDETLCG
                     DNINGLTDSVIYLQKSYRFSITGGIGMCAEAVKTGNHKLFMELCSKNNSQVVICDGGK
                     NIRESILQVIGKIEKDTGIQYGSFKSQQNIKDKIESFNRLMFLCAVRKSDRGVESVNN
                     TLKNIITNDLKISGNAEPLIVTGNMYDLGLYNGDTGLVINEKGVDWFYLENESGESLK
                     RVNYSLISSCEPVYSMTVHKSQGSEFDNIVIILPDYMSPVLSRELIYTALTRARERVY
                     IISSSEVLEEAVKNERKRMSGLGYFLER"
     gene            complement(13623..17237)
                     /gene="recB"
                     /locus_tag="P5123_03305"
     CDS             complement(13623..17237)
                     /gene="recB"
                     /locus_tag="P5123_03305"
                     /EC_number="3.1.11.5"
                     /inference="COORDINATES: protein motif:HMM:TIGR00609.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009338 - exodeoxyribonuclease V complex
                     [Evidence IEA];
                     GO_function: GO:0008854 - exodeoxyribonuclease V activity
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease V subunit beta"
                     /protein_id="HRX15318.1"
                     /translation="MTDTKNLYKELDLSKPPIPGRNLVEAGAGTGKTFSIALLFLWQV
                     IYREMSVDQILVVTFTDAATSELKDRIRKFLNIALDVYAGNNDESNEMACVRNICMYK
                     DPDSNQIERRKIITARAIHNFDDVSIFTIHSFCQRMLSENAFETGSPFTTSVIENQDE
                     VVRQLSDDFYRSYTYKQSDLSFALLEEYGVSLKYITKLAREYTKHISLKITPSISQQL
                     VKNVAISFASDNLEELYVKTKENYNEKISQIDPSWEKEVETFFKDDFKFKKKYKFENL
                     IENLSQFFNNTETDYPLSDNFRDIISFFSRKTIIEKAFLKKFEEEPVQHPFFDFCEEL
                     DEICLQYEGQKDEESTARKTALIHYMLKIIKTRLDSHKKERHLISYNDMLSNLDKAVN
                     NNKDLIKTVSNRFSSVFIDEFQDTDPMQYNIFSTLFSNMNTYLYFIGDPKQAIYSFRG
                     ADLETYIEAAKSCKERYSLTTNFRSTSDIIDPINRIFSNNNPFIEKSIAYRKALSAKG
                     KSSDFIIEDGRSNFIVSLIDNFNKSESEQHIADNIALRIKEILALSEKKSAMIGDKSD
                     DGEKIKRPIMPADIAVLTRTCKQGSKVAASIREQGINAIEINPVSVYSSEEFFEIELL
                     AESLSDPYCMKKLSALLCTRICGLTVNDIDDIAHNDSEKYNGYLLDIRRYAELWKKSG
                     FNTAVCAVFEDFDTYNRILSLSNGDRIITNYNQLIEIFNRYCLRNSASPDELLFYIQM
                     LKNEDIDDDDREQPVRMESDEQAVIVSTIHKSKGLEYPIVFVPYCWNNSEPFANNNFV
                     YHKNGERCLEIVGGNDCDNYRKCANEILAENIRLLYVSLTRACNRLYLYWGNVKNNKS
                     NFYSSALDYLFFANMLDESEDDNSDDYVQKLRSLLKTDKPDLSGILKEKFDGLNIDIE
                     HIDNSEHDSDKLFSKTEKVTNFKSRVFDRAIDRSWRINSYSYITSGIYDESGHETHLP
                     EQKRDENMLPPGASAGNCFHKVFENIDFINDIGEQLHIIEDALNRYGFDLCYKDYMQQ
                     CVSSVLKAEVMPGFKLSMLNSRDRVHEMEFYFAINNVDITRLVKIINKEYPEFVTNET
                     MIVSEINGFMKGFIDLTFVYKDKIFIADWKSNYLPGTYSESEVKDSMRRHLYHIQMII
                     YSIALWRYIKSRCGSFDYSMFGGVLYLYVRGMREDCSSGVYFDRPKEQFLIEVERCFV
                     "
     gene            complement(17239..>18652)
                     /locus_tag="P5123_03310"
     CDS             complement(17239..>18652)
                     /locus_tag="P5123_03310"
                     /EC_number="3.1.11.5"
                     /inference="COORDINATES: protein motif:HMM:NF037683.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="exodeoxyribonuclease V subunit gamma"
                     /protein_id="HRX15319.1"
                     /translation="LPMRSIPFKVIAVIGFNEAAFPRKGRKGSYDLLSRYPAIGDRSM
                     RFDDRYLFLETILSARKFLYFSYQSFKESDNSKLFPSPLLLELLHYLEENCDNFEAEK
                     FIVVHKLQPFSSGYLDNSDSRHFTYDSRQIERAKIYYGGEKNSYSYFSDSLSSTPEHN
                     FQTTNIGMIIDFFENPCRFLLRKRVGIDFTIHGNEYNNSEDLSLGNSSKGQIIKMLVN
                     RLIDGKCIDDYYRVLSMNGLLPLFASGKYEYLKIKQGSQLIFDELNKLTKVNSFYECE
                     ATYSSSINTEYKKDSAFSVSSNLDFVNEEGRFVIIPNGLKYIKYNIARIWLEHLLFQF
                     SKNSCKQTFVVSEEGILLFNIIKKSDADNYLSNILSLYSQGLKSPLRFSLNDSLGLYK
                     EINKESKKEKSVDDLLSELMKTPYENTDKVYNDYIAKQKRYQELCFENSIDLYTKEFA
                     ENSLQFFNGIKESCPEILKV"
BASE COUNT         5709 a         3707 c         3086 g         6050 t
ORIGIN      
        1 attggtatgt tgagccttta gtccggaaac gacaagggta aatatcaagt ctttgcgtct
       61 gataatttca tgtaaatatt ttttcataat tgatccgaaa ccagcaaacg tgctggtttt
      121 actttttatt atttgttgct ataaaataag aaaatttgga tttttacctt ttctgatatt
      181 tgtcgattcg gtgcaacctt tttttgttca ttatggggac ggtgcattat gcattatggg
      241 gacggtgatg tttcgggatg gcatttggga cggtgatgtt ttcggtccgg acgacgggga
      301 cggtggtgtt ttccggacaa cggggacggt gatgttttcg ggatggcgac ggggacggtg
      361 atgttttgtt aaagtctata gttttatagt tacccgtctt gtttgtctca atattggttt
      421 gaaaaatggt ttgactcatt ttccatgaaa aagtgtattg agattatacg aataattttg
      481 ggacagtagt aatctggctt gggaaacgga gacagagatg ttttggttcg gcgcggcgga
      541 tctggaatca gggacggtgg tgtttcagag acagtgataa ttagtaagta aaaacaaggc
      601 aaatccatga aaatagaaga atacagaaaa ctttgtcaaa aaaatgaaaa cgaaaactat
      661 ttttttgaca aatatgtttt tagaaaaata tccatatttt ttaccatttt ttttactttt
      721 actggtgtaa acgcaaatac agttacattt ttgtcattat tgtgcagtat aggtgccgcc
      781 tggtttgccc tttttaacac agatacagcc ctttcactgg ttgttattct tatattttta
      841 tattatatcc tagatcacgt ggatggggag cttgcccgct atcttattaa caagggcaag
      901 atgaaaccat cacttgttgg tgcatatttt gatcagctag tacataaata ttcggcaaat
      961 ctgattcttt tttcatttgg atacgctgtt tttacagaat acggatacac gtgggcacta
     1021 tttactggtt ttatctgctg tattggccta tccggttttc ctaacctgct aaattacaaa
     1081 gtaatattgc aaaaaatcat cactgacccc aatattatgc aaaaagcaga atataaggag
     1141 ttgcttgatc gctgtgatca gaaaggtaat tcgattaaaa tcattcaaaa aggattgggg
     1201 ttaaagaaat acaaaaaaat tgcgggggaa atacttgtgt ttccgggcgg tgttttactc
     1261 tgtgcaatag cggcactctt ggatcttgtc tttaaggata tcatctacat gggattcggt
     1321 tttaatttta gacttctgct catcgtcggt cttaccccgg tgtatcttct taaggtggtt
     1381 ctagattgga gaaaatggat gaaacagcta aaaaacgcgg gttaaaccac taacgatcct
     1441 gtaaaaatct taataatctt tccgatgaat ttccgtcttt ataggtattt aaccggttta
     1501 ccatctcttt atattgctct ttgaactttt tttctttgct ccatctttca acagaaagag
     1561 caagtttacc aagagcgtct tcatccaaag caaatgggcc tgaataagcc tcgtcgggct
     1621 ccagatacag gctacggcat ctatttctgt actcttctac atcataggtg taaaatatca
     1681 caggacgccc agtgaggcaa aaatcgttgg taacactgga atagtcggta attagaacat
     1741 cagttaccag taataatggc tgaagatcta caacaatgtc tgagcaatcc tttatatatt
     1801 caagatttcg gggaaccaca atgtttttat cgtatggatg tttcttgata gccaataacc
     1861 aatttctttt tttaaattct ttttcaattt gtgcataaaa cttatccgaa aatggtctaa
     1921 ccatcgtata gttatcacga aatgttggaa cataagtaaa aacttttctg taatttttcg
     1981 tcaaatctaa cacttttaac ggaattggat aactctgata aaaaatatcg ttacggggat
     2041 aacccaagat gtgtactctt tttgacccaa acgcgctttt acagatgtca gccgtcattt
     2101 gggaagtagc aataatatat ttataggggc gatacaaaaa gtatttcttg aaatcccttt
     2161 ttaacatgtt ggcaaaccta tcaaagattt ttatcttaaa cagattaaaa ccagctatcc
     2221 ccttgccttc acgcgccgca tctaagccta tttttttaag agctatcccg tgaaaggtct
     2281 gtacataatt gaaacaacca atgcttgata aaccgaaata aagaatatct cgtggcatct
     2341 gttcaataac aagaaattct gagcggagta tccatgtaaa tgctttaaac gaatatttat
     2401 ataaaaccgg atacccatca ctctccatgg atgcaaaaag ctctttgcag ccggttatcc
     2461 agtatgtgtt ttgatgatta gcatcaagca ggtatctgta aaaatatgcc ggatttccaa
     2521 taaactgatc cccgcgtgaa gcacccatca aaaaagaccc ttttttttta ggtgcaacca
     2581 acgacagaaa aataagaaga atatccaata atatatctat caccatcatt aaatgtttgt
     2641 aaaagctcaa gtttttatgt caagtatttc tgagttctaa ttttatattt taacatatgg
     2701 gaacatatgg ggacggtggc gtttccgagc atgtggggac ggtgatcctt ccgagcatat
     2761 ggggacggtg gcgttttggg gcaacgggga cggtgatcat tccgagcatg aggggacggc
     2821 ggtgttttgg ggcaacgggg acggtgatca ttccgagcat gaggggacgg tggcgtttcc
     2881 gagaatatag ggacggtggc ctttccgagc atatggggac ggcggtgttt tgacataaaa
     2941 gagacagaga ctaagatgta attcattcat tttttttgcc ttttcaaaaa aaaattttaa
     3001 caaaatcaat aaaaattcgt ttatatagtc agtggcattt tttgacaccc ttaatcttat
     3061 acttaataac ctcagtcaaa gcacattcct aaaaattaat taaactaata attatatgta
     3121 aaggacggtt catgatgaga aaaccaagag aaattgttga tggatcaaca tatcatgttt
     3181 ctgcgaaaat aaatcgtggg gaatttatct tcaaagacag agccgatttg aaaattctat
     3241 ttttcaaact tgtaaagaga actcatggaa aatataaatt tcatgttcaa gggatcactg
     3301 tgatggataa tcatatacat atgacgatta aaccgagtaa aggggaaaag ctcggtaaca
     3361 tcatgaattc aatactaagt acatttgcaa gaaggtataa cgttattatt aacagccatg
     3421 gtcatgtttg gtatgatcgt tttaaaagca aagtaattgt tacttttact caatacataa
     3481 acactctaag atatatttgc aataatccat tacgagcagg catggttaat gatccattaa
     3541 gttataaatt ctgtgctgtt aatgctctgt acaccaagga gaaaagcgaa ttggtatcaa
     3601 taattcaaag cataattgat ttcaaaaaca aagagctatt aaatatttat gatgattata
     3661 taaaagaatt cgatgtcaat aaggctattg aaaaagacat aacaataggt ttttatccta
     3721 ataatccagg gcggcccaaa aaggataaaa aatgatattt ttggcaaaaa ttataaaata
     3781 tggaattttc agttaagcga agaatcagag agttctgaag gagagagtgt caggagagtg
     3841 taaaaaagat tgtgtaaata atcatttacg tatactatat ttcactcttt catcaaaatg
     3901 aattacaatt tccacttgta tttaactgag ttcttcgatc tgagcattgt ccattttctg
     3961 caagtaaaac cgaaacgaca ttatcacttt agatcagtaa cggtctttgt cacttattag
     4021 cagcaaatac ctccgccccc gttttaccgc atctgttttt taacactgtc cctttagtca
     4081 cgtacgaaaa caccaccgtc cctgtacgac caataacttt tcgtaacacc accgtctctg
     4141 tacgaccaat aacttttcgt aacaccaccg tccccattaa acctccgtct aaaacctccg
     4201 tccccgtttt acatcacatt gcggaacact gtcccttttg tcccctacga aacgccaccg
     4261 tccctgaagc tcggtccctg agctcggtcc ctgtaaaagc tcacatttta cgcatatagt
     4321 catcatcaac gcgaattata tcatcttcgc caacatattg accaacttgt gtctcgatta
     4381 tgttcaaggg aaccatccca ttattggtaa gtctgtgaga atcaccgatt ttaatgtata
     4441 ctgattcatt tggtcgtact atctgttctt tgccctctat ctcgacagtc gcggttcctg
     4501 taacgactac ccaatgctca ctgcggtgca tgtgtttttg taaagaaagt cggcgaccgg
     4561 gtttaaccat aatgcgctta attttgaaat tactgctctc ttctaatacg gtgtacgttc
     4621 cccatgggcg atgcaccgta cgatgtactt cggtaagttc tgtcttgtgt aaaccaccgt
     4681 cccctaagat atcgactatt tcttttactt tttgtgaatc accgcgtttt gcaatcatca
     4741 aggcatcatc agtatcaatg ataatcgaat cactaattcc gatggtagaa attgttctgt
     4801 cgcaaccaat tattagattg tttttactgt cgatagaaac atgattggta tcaacagtat
     4861 tacccgcatt atccttaggc aaaacctcgt acaaagagtc aaaactcccc agatcattcc
     4921 agccaaaatc agatctgatt actgctccct tatcagattt ttccattacg gcataatcaa
     4981 tactgtcagc aggtatggcc gccatttttt catatggaac gaagagtgag tctgtatcct
     5041 gctcaaaacg gcgttcagaa ttattatatg catttcgaga tgcttcataa atctcaggtg
     5101 catatttttt taattcatca agaaaaactt ttgctttaaa gataaacata ccgctattcc
     5161 agtaataatt tccctgttgc aaatactctt tggcagtatt ttcatctggt ttttccttga
     5221 acgctttaat tagataattg tcattgtccc caagtttttt tccggcttcg atatatccaa
     5281 atcctgtttc agggtatgag ggagcaatcc cgaatgtgac aagatatcct tcagttgcaa
     5341 gtttttcagc ctcgatcgcg tttgcaaggt aattttctgt atcttttatc aaatgatcag
     5401 aaggagtaac aagaacgaca tcatctttat tacaatccat acatgcgaga gctatagctg
     5461 gtgctgtatt tcgtccaaga ggttccagta taaatcgggt catgttttta atagagctaa
     5521 ctccggaatc ctcactctga tcggcagtca taaaatgcag atccctgtta gtaacaaaaa
     5581 taaatttatc gcatatagaa ctattacgaa caactgtttt ttgaaatagc gatttgcctt
     5641 caaacaaagg gacaaattgt tttggataaa gcttcctcga taatggccaa agtcttgtac
     5701 cgcttccacc acataacact atattaacca ttattaacct tcctgtattg taaaaataat
     5761 tattctaaac ttcaggtctt ccaacactga tatatgtgaa ttctctttct ttcatcgaag
     5821 agcacttgta ctgattacgc ccgtcaaata taacaggttg tcttaataga ctctttattc
     5881 tttcaaaatc cggacgtcta aaatgatgcc actcggtcat tattattaaa gcgtcagcgt
     5941 cttttaggca atcgtaattg ttgtttacat aatcaattcc agaatcaggc aataaaggtt
     6001 ttgcgttatt aaccgctatc ggatcatatg ctgtgaaaat tgctcccaat ttataaaggt
     6061 aatttataat atctacagct ggtgcttcgc gcatgtcgtc agtctgaggt ttaaaagcca
     6121 atccccatac ggcaaatttt tttcctttaa cagctccacc ataatggtca actatctttt
     6181 gagcaaaaat ctgtctttgc tcaagattta cctcttctac agcattaaga attttagact
     6241 ccatgcccaa ttgttttgag gtacgtacaa gtgccttaac atcttttggg aaacaggaac
     6301 caccatagcc aataccggca tacaaaaatg aacgcccaat tctggaatca gatccaattc
     6361 cttttcttat catttcgaca tttgctcccg tagcatcgca aatacgtgac atctcgttca
     6421 taaaactgat tcttgttgcc agcatacagt ttgccgcgta tttagtcaat tcggctgagg
     6481 gtatatccat actataaaat ctatcttcac tgcgcgtaaa tggcgaatag agttctttca
     6541 ttagttctgc aacacgcaca ttatcacaac caacaataac acgttccggt ctcataaaat
     6601 catcaacagc gtcaccctct tttaaaaact ctgggttaga aacaacatca aattcaatat
     6661 cactactatc tctttcagct agaactactt ttattttttc acgcactttt tcagcagtac
     6721 ctactggaac tgttgacttg ttaattacga tagtataccc tttcatatta tttccaatat
     6781 cattggctac acttaataca tattgaagat cagctgatcc atcttctcct gggggcgttc
     6841 ctaccgcaat gaaagcaaat aaggattttt ccaagccgtc gctaacggag gtagaaaagt
     6901 ttaatctacc ttcagaataa ttacgtttaa caatttcttc cagtcccggc tcgtaaatag
     6961 ggattatacc ttttttaaga ttctcaatct tattctcatc tatatcaatg caccagacat
     7021 cattacccat atcagcaaaa catgccccgg tgacaagccc tacgtatcct gttccaacaa
     7081 ctgtaatctt catagcttct cctaaatgtt gcagtattac aacactgttg caaaatcaga
     7141 acatgtttac aacagtaaca attttaaaat catgactatc agatatttta tccatgattt
     7201 tacaagataa atttttacat cattgattat ggcaatagaa tttcattggc aggtacttta
     7261 ttcggcacta tgttaattat ttcaaataca aatacgaata tagtaatatt tgcagttaac
     7321 caattataat gtttaattct tttattaaat ccataatgac tacattattc ttggatattt
     7381 cttttaaata attatcgata aataattgcg actcttcatt acaacaatat tcaggatgca
     7441 atcctgtaat atttggattt accgtttcac cttttttttc attatcataa gaaagctttt
     7501 ctctgagctt ttctccaggc cttaatccgg tatattctat tttgatgtct atttcaggtg
     7561 tcagtccttt cgatttaata atattttctg cgatctcttt tattaagtat tctcttccca
     7621 tattgagaat ataaagagga tgactcttca acacagccgc gtgaattaca agtaaagcag
     7681 cctccggtat tgtcataaaa tatctacgca tttcaggatg agtaattgtt acaggccccc
     7741 ctcggtctac ctggttatta aaaagtggta taacagaacc gcgactacca agaacgttac
     7801 cgaatctgac caccgatgtt ttcatttttg tgttatacga catagtgaga atttcaccgg
     7861 cacgttttgt tgctcccata accgatgatg gttctaccgc tttgtcggta gatataaaaa
     7921 caaatgattt tacttcattt ttttcagaag aatttaatag agtacttgtg ccgccaatat
     7981 tattgagaca tgcttctcta atattgaatt ccataagcgg aacatgctta tgtgctgcag
     8041 cgtgaaaaac aacgtccggt ttgaattttt ttagaatcga atctatcgaa gagccatctc
     8101 gaacatccgc gattacataa ctaacactga tttttttatt gaatccggtc atggtttgtg
     8161 aaagattata gatagatcct tctccatggc caagacaaat aagactatct atatttaacc
     8221 tcattagcat acggcaaagc tctgatccta tgctcccccc agcaccggtt actaaaattt
     8281 tttttccaca atactctttt gatagcagtg tggtatcgca agtaatttcg tcccgatcaa
     8341 taagtttaat atatgatgag tcttccaact catttattaa tggagaatcg aagaatctgg
     8401 ttacataggg aagtattttg acattaatgt tgcgatcata ctgtagaacc ctgtaaagtg
     8461 cgttattgat ctgttcggta ttcgcggaag gtatcgcaac gataaactgt tcaatgttat
     8521 cactctctac aattttttca atatccttac tctctccaag cactcttatt ccgtttatac
     8581 tggtaccaat aagcttactg tcatcatcca caaatcctgc tacagtaatc gaactttcca
     8641 ataactcggt ggcaatactt tggcctgcag agcctgcgcc ataaatcaaa acagctcctt
     8701 tattcatttt atctcccttc cacacaatct tcggctgttt taatcacagt ttttgctacg
     8761 taattaactt cgttatctga taatcctgga taaataggca aagaaatttc acgtttatat
     8821 acccagtcgg caccaggata attatgggca tcaagtttgt ctttataata gctgaattca
     8881 taaaaaggaa taaaatgcaa cgagcttgat actccttttt ctaacagttt ctttgaaaag
     8941 ttatgcctat caatttttaa ctcatcaata tttattttta atgggtaaag gtggaaggca
     9001 cattctacat aattttcaat ttcatatggt agtatatatg ggttatcaga gaaatatttg
     9061 tcatatattt ttgcgatttg ttgacgtttt tgcaaggaac tgccgcatct acgaagttgt
     9121 gataatccca gcgcggccgc tgtgtcggtc atattgtatt tatatccgtt ttcagtaaca
     9181 tcgtaaacgg gacttccgcc ttttccgtat cggttccatg cgtcacgggt tattccatgc
     9241 aggcgaagag agcgtattct gtcaagcttt ctggaattgg caccaacaat cattccgcct
     9301 tcgccagtag ttatagtctt tgttgcgtaa aaactaaaac atccgtaatc acctattgtc
     9361 cccaaggcac gccccttata agtcgcagga aacgcatgtg ctgcatcctc gataacaaca
     9421 agattatgct cagcggcaat actcattatt ggatccatat cagccgcgtt tccggcatag
     9481 tgcaccggta ttatcgccct ggtattctct gtaatcaggt ttctgacatg atttgtatct
     9541 attgtatgat tatctttttc aatatcagca aaaacaggag ttgctccggt atataataca
     9601 gcctccgcag ttgctacaaa tgtggttatc ggaatgatta cctcatctcc tggacctata
     9661 ttttcggcaa caagtgcgag gtgaagtgct gcagtacaag aacttaccgc aacagcttca
     9721 tctttctcca aaaaagccgc gaataatttt tcaaattcga aagttcgagg ccccatcgta
     9781 agccaaccac ttcgcatact ttcgacaact gcatcgatat cttcttctgt gatatccact
     9841 ttatgaaaag gaatttccat ataattttcc tgccgggaat ataaagattc aaaacagagc
     9901 aaataattat gcnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     9961 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nncatataat
    10021 tttcctgccg ggaatataaa gattcaaaac agagcaaata attatgcccg tgcgttattg
    10081 agtcaaacaa aaatcatcac cgcctcaaat accagagtaa ttacccgaaa aacaggtcac
    10141 cgtccccaat ttcccggcag atcacagaca gaaaacgtca ccgtccccgt tttccggcaa
    10201 attacagaca gaatacgtca ccgtccccgt tttccggcaa atcacagaca gaaaacgtca
    10261 ccgtccccaa tttcccggca gatcacagac agaaaacgtc accgtcccca atttcccggc
    10321 agatcacaga cagaatacgt caccgtcccc aatcaattag ttccgttttt gttcaatgcg
    10381 aaccggaaat catttcctgt cggattctga aatattttta aatcgaacca tgatgctgca
    10441 gcaaggatat gatcaaaaat atcggcctga atttcttcat agtttaccca atttgtatcg
    10501 tttgtaaaaa cataaagctg cagcggcaga cctttttcgg aaggcggcaa ctgtcttaca
    10561 atctgaatca ttccgtcatg aatcttaggg tgattcttaa gataatttgc aatataatgt
    10621 ctaaaaaccc caatattggt catacgtcgc ccgtttatcg gtaccgatgg atcagctcca
    10681 aaacttttgt tgtattcttc gatctctttt tgacggcaat caatataatc acgcaaatgg
    10741 aaaatcttta agtacgaatc aagcatcccg tcagtacaga attttatgct gctgacatca
    10801 atatgcacag atcgcataat ccgtcgccca cccgactcct gcatacctct ccagttttta
    10861 aaagagttcg aaacaagcgc gtatgtcgga attgttgtaa ttgttttatc aaagttctga
    10921 acttttactg tatttaacga aatatcaatt acatctccat ctgcaccata tccgggcatc
    10981 tctatccagt caccaacccg taccatatcg ttagccgaca attgaacacc gcctacaagc
    11041 cccaagatgg aatccttaaa aaccagcatt agtactgcag ataatgctcc aatacctcca
    11101 agaagcagcg aagggctttt tcccattatt ccggctatca taagaacgat tgcaattacg
    11161 gtagcaaata ttttggccac ctgtaaaaaa ctttttatag gacgggtttt ggatattgag
    11221 taggagttgt atatatcgtt gacggcatca agaagcgcgt acaaagttat aagagctata
    11281 aaaataatgt atacctttac ccctctattt aacggttcct gcaaatagtc aggaaaaaaa
    11341 ttaataaaaa tataaattat aacggcagga acacagtggg ataaacgtga aaaaacctta
    11401 cgctcaagca taatgttatc ccaggtaaaa cggttatttt taatgtacca ggaggcagct
    11461 ttaagaataa tcttttttgt gatgaaattt gccgtaaaac ttaatagaaa cacgataaaa
    11521 gccaatacaa aatatgtaat catgttcaga taagcaggat caaaattaaa ttgtgacaac
    11581 aactcattta aataagaaac cagcatttta aaccccgttg atattttttc tatattgttt
    11641 aattattttt ttaactgtca agaaatttca ccgctccaga aaatatccaa gccccgacat
    11701 tcttttccgt tcatttttca cagcttcttc aagtacttca ctacttgaaa taatataaac
    11761 gcgttctcgt gctcgcgtca aagccgtata tataagttca cgagatagaa ccggcgacat
    11821 gtaatcaggc aatatgataa caatattgtc aaattccgag ccctgactct tatgtactgt
    11881 catgctgtaa acaggttcac aactgctgat taacgagtaa tttacacgct ttaacgattc
    11941 tccgctctca ttttccagat aaaaccagtc tactcctttt tcattaatta caagtcctgt
    12001 atcaccatta tataaaccca gatcgtacat gtttcctgtt acaattaacg gctcagcgtt
    12061 tccagatatt ttcaaatcat ttgtaataat gtttttaagg gtgttgttca ccgactcgac
    12121 ccctctatcg ctctttctaa ctgcgcaaag aaacataagt ctgttaaaag attctatttt
    12181 gtcttttatg ttctgctgac tcttaaatga gccatactgt atgccggtat ctttttcaat
    12241 tttacctata acttgtagaa tgctttctcg tatgttttta cccccatcac agattactac
    12301 ctgtgaattg ttttttgaac ataactccat aaaaagttta tgattacccg ttttaacagc
    12361 ctcagcacac ataccaatgc ctccggttat cgaaaacctg taactttttt gcaaatagat
    12421 cacagaatca gttaatccgt ttatattatc gccacatagc gtttcatcaa tattaacgcc
    12481 acaatttacg gcaaatttct taaactcatt tgaataaagt tttccattat tgcgatagca
    12541 tatatctgcc atgatcgcac cagcatcaac agacgccagc tgcctgctat cacctaacaa
    12601 tatcagtatg ctatcatctc gaagccgaag cagtagtttg tagagcaatg acaatgacat
    12661 cattgatgcc tcatccacaa tcacaagatt ataaggtaat ttttctatat gttgatcaat
    12721 atgttgccga gccgaaccaa taagacggtg aacagttaac gcctcgatat catcatcttt
    12781 aagccatttt tttaaattct tttccatgga catatcttcg gttacaacgg accttgcctt
    12841 actaaaagca gtaaacattc gagccgcagc ctttccggtc ggagccgtta gcacagcagt
    12901 aaagctgttc cccttcatcc tttcgattgc tgccaaaagc atatatattt tgtaaacagt
    12961 tgttgttttc ccagtaccag gaccacctgt gataatagta agatttcttt ttaatgcggc
    13021 acgagcagcg atatattgcc aatcagcttc tttattgttt tcgttatagg caaatatctg
    13081 tttcgaaact ttatcaatct cttcagaaac atcgtttttt atctgtgatg ctttgtccaa
    13141 taataacaag gcacattttt tttcctgaaa aaattgtttg taaaaataga gaagcccgtt
    13201 aaaatagaca acaggagtta cagagctgcc tgtgccgacg gttttgaatt tattaaaaaa
    13261 atcttcacta acaaatttta caagctgatc tgccaattca tttgtcatat taaccacaga
    13321 gtcattatac tcgtaaagat tatcatcatc gaaaggacta acaccaagct ggtagcagac
    13381 atgcgcattt aacgtaaccc ggcttaatgc taaaagtata agatggtaaa gaggatcgta
    13441 aaactcctcc tgttccagaa tataatcact gcttaatcga tccgcttcac aaaatatttc
    13501 ttctataaat agttgcgaat atctgttata gaaatcatct tttgacttaa tattattgaa
    13561 agtggtagag cttgttaact ttatatcttt agcattatcg atagttgttc cgttcttttt
    13621 gctcatacaa aacacctctc gacttcaatc agaaattgtt ctttaggtcg gtcaaaataa
    13681 acaccactgc tgcaatcctc tctcatgccg cgaacataaa gatataaaac cccgccaaac
    13741 atcgaatagt caaaacttcc acagcgagat ttaatatatc gccaaagagc aatcgaataa
    13801 ataatcatct gtatatgata taaatgcctt ctcatagaat cttttacctc tgactcggaa
    13861 tatgttccag gcagatagtt gctcttccag tcagcgataa atattttatc tttataaacg
    13921 aaggttaaat ctataaagcc cttcatgaaa ccgtttattt ccgaaacaat cattgtttcg
    13981 tttgtaacaa attcagggta ctctttattt atgattttga caagccttgt tatgtcaaca
    14041 ttgttaatag cgaaatagaa ctccatctca tgcacccgat cacgagaatt taacattgaa
    14101 agtttaaaac ctggcatcac ctcagccttt aagacagaag atacacattg ctgcatataa
    14161 tctttatagc acagatcaaa gccatatcgg ttaagcgcat cttcaatgat gtgaagttgc
    14221 tccccaatat cgtttataaa atcaatattt tcaaatactt tatgaaagca attaccagcc
    14281 gatgctccgg gtggcagcat attctcatca cgtttctgtt ccggcaaatg agtctcgtga
    14341 cccgactcat catatatacc agaggtaata tagctgtaac tattgatcct ccaacttcta
    14401 tctatagctc tatcgaagac tctgctttta aagttggtta ctttttcagt tttagaaaac
    14461 agtttatccg aatcatgttc actattatca atatgttcga tatcaatatt taagccgtca
    14521 aatttttctt ttaatatacc tgataaatca ggcttgtcgg ttttcagcaa agagcgcaat
    14581 ttttgcacat aatcatccga attatcatct tcactctcat caagcatgtt ggcaaagaaa
    14641 agataatcaa gggcggacga atagaagttg gatttattat tttttacatt tccccaatac
    14701 agatatagac gattgcaggc gcgtgtcaac gatacgtaga gcaatctaat attttcggca
    14761 agtatctcgt tagcgcattt tctgtaatta tcgcaatcgt ttccaccaac aatttccaga
    14821 catctttcac cgttcttatg ataaacaaaa ttattattag caaatggttc tgaattattc
    14881 caacaatagg gaacaaaaac tatcgggtac tccaagccct tactcttgtg aatcgtagat
    14941 actataacgg cctgttcgtc gctctccatc cgcaccggct gttctctgtc atcatcgtcg
    15001 atatcttcat tttttagcat ttgaatatag aaaagaagtt catcgggact cgcactattt
    15061 cgtaaacagt agcgattaaa tatttctatc aattgattat agttggtaat tattctatca
    15121 ccattgctca aagaaagaat tctattgtat gtatcaaaat cttcaaaaac agcacagacc
    15181 gctgtattaa aacctgattt tttccataac tccgcgtatc ttcttatatc aagaagatag
    15241 ccgttatatt tttcactatc attatgagca atgtcgtcaa tatcgttaac agtaagtccg
    15301 caaattcttg tacataaaag tgccgaaagt tttttcatgc agtatgggtc agaaagagat
    15361 tctgcaagaa gctcaatttc aaaaaactcc tcactgctgt aaacagatac gggatttatt
    15421 tctattgcgt ttattccctg ttcacgaata gacgctgcga cttttgatcc ttgcttacag
    15481 gttctagtaa gaactgcaat atcagcaggc ataataggcc gtttgatttt ttcaccgtca
    15541 tcagatttat cgcctatcat agcactcttt ttttctgata aagcaagaat ctctttaatt
    15601 cttaacgcaa tattatctgc aatatgttgt tccgattcag acttgttaaa attgtctatc
    15661 aaagaaacaa taaagttcga acgtccatct tcgataatga aatcactact tttcccctta
    15721 gcggataacg cttttctgta tgcgatgctc ttttcgataa acggattatt attgctaaat
    15781 atgcggttta tgggatcgat aatatcagat gtcgatctga aattagttgt taatgaatat
    15841 cgttctttac agctttttgc agcctctata taggtctcaa gatccgcgcc tctaaaagag
    15901 taaatagcct gtttgggatc accaataaaa taaagataag tgttcatatt actgaaaagt
    15961 gtagaaaaaa tattgtactg catcggatcg gtatcctgaa attcatcaat aaaaacagag
    16021 ctaaatcgat tgcttacggt tttaatcaag tctttgttat tgtttacagc cttatcaaga
    16081 tttgaaagca tatcgttata gcttatcaga tgtctctctt ttttatgcga atcaagtctt
    16141 gtttttatga tctttaacat ataatgtatc agagctgttt ttctggccgt actctcttca
    16201 tctttttgac cctcatattg caggcatatt tcatcgagtt cttcacaaaa atcaaaaaaa
    16261 ggatgttgta caggttcttc ttcgaatttt tttaagaacg ccttttcaat tatagtcttt
    16321 ctgctaaaga aagatattat atcacgaaaa ttatctgaaa gaggataatc tgtttcagtg
    16381 ttattaaaaa attgtgataa attttctatg agattttcaa atttgtattt cttcttaaat
    16441 ttaaaatcgt ctttaaaaaa tgtttcgacc tctttttccc atgaaggatc aatttgtgaa
    16501 attttttcgt tatagttctc ttttgtttta acataaagct cttcaagatt atcactggca
    16561 aaagatatgg ctacattttt tacaagctgt tggcttatac tgggagttat ttttagagag
    16621 atatgttttg tatattctcg ggcaagcttt gttatatatt taagggacac tccatactct
    16681 tctaacagag caaaagagag atcgctctgc ttgtaagtat acgatcgata aaaatcatct
    16741 gaaagttgac gtacaacttc atcctgattt tcaataacag atgtcgtaaa aggtgatccg
    16801 gtttcaaagg cgttttcgct cagcatacgc tgacaaaaac tatgtattgt aaagatagat
    16861 acatcatcaa aattatgtat tgctcttgcc gtaataattt tacgtcgctc gatctgattt
    16921 gaatcgggat ctttatacat acaaatattt cttacacaag ccatttcgtt tgactcatca
    16981 ttattgccgg cataaacatc aagcgcgata tttaaaaact tccttatacg atcttttaat
    17041 tcactagtag cggcgtcggt aaatgttact acaagaatct gatctacact catctctctg
    17101 tatataacct gccacaagaa aagcagggcg atcgagaatg ttttaccagt accggcgcct
    17161 gcctctacaa gatttcgtcc tggaatgggt ggtttggaaa gatcaagttc tttatataaa
    17221 ttttttgtat cagtcatctt ataccttcaa tatttcggga caactctctt ttattccgtt
    17281 aaaaaactga agagagtttt ccgcgaactc ttttgtatat aggtcaatac tgttttcgaa
    17341 acagagttct tggtatcttt tttgtttggc tatatagtcg ttataaactt tatctgtatt
    17401 ctcataagga gttttcataa gttctgataa aagatcatca acacttttct ctttttttga
    17461 ttctttgttt atttccttgt acagacctaa cgaatcgttt agagaaaaac gaagcggact
    17521 ttttaacccc tgactgtata agctcaaaat attagataaa taattatcag catcactctt
    17581 ctttataata ttaaagagta gtattccctc ttccgaaaca acaaaagttt gtttgcaact
    17641 gtttttgcta aactgaaaga gtagatgttc tagccaaatt cgtgctatat tatatttaat
    17701 atatttaagg ccatttggaa taataacgaa acgtccttct tcatttacaa aatccaaatt
    17761 cgaagataca ctaaaagcag aatccttttt atactccgta tttatactac tgctatacgt
    17821 agcctcacat tcataaaagg aatttacctt ggtaagttta tttaactcat caaatatcag
    17881 ctgtgagccc tgcttaatct taaggtattc atattttcct gacgcaaaaa gaggcaatag
    17941 cccgttcata gataatactc ggtaataatc atctatacac ttaccatcga taagccgatt
    18001 aaccagcatc tttataatct gacctttaga gctgttaccc aatgataaat cttcgctatt
    18061 attatattca ttaccatgta tagtgaaatc tataccgacc cgctttctta acaaaaaacg
    18121 acatggattt tcaaaaaaat ctataatcat acctatatta gttgtttgga aattatgttc
    18181 aggagtcgaa gagagcgaat cagaaaaata tgagtaacta tttttctcac ccccgtaata
    18241 tatttttgct ctttctattt gacgagaatc ataagtaaaa tgtcgactgt cactgttatc
    18301 aagatatccc gacgaaaacg gctgcaactt atgaactaca ataaattttt cggcttcaaa
    18361 attatcacag ttttcctcaa gataatgtaa aagctctaac aagagcggag atggaaaaag
    18421 tttactatta tctgattctt taaacgattg gtaactaaaa tagagaaatt ttctggcact
    18481 taaaattgtc tcaagaaaaa gatatctgtc atcgaatctc attgatcggt cgccaatagc
    18541 aggataacga ctcaaaaggt cataacttcc ctttctgccc ttgcgtggaa aagctgcttc
    18601 attgaaccct ataacagcaa taaccttaaa aggtattgaa cgcatcggca gc
//
LOCUS       DAOTGZ010000028        18283 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_3064
            NODE_3662_length_18283_cov_7.014922, whole genome shotgun sequence.
ACCESSION   DAOTGZ010000028 DAOTGZ010000000
VERSION     DAOTGZ010000028.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868088
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 18283)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 18283)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:22:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,785
            CDSs (total)                      :: 2,751
            Genes (coding)                    :: 2,732
            CDSs (with protein)               :: 2,732
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 12 of 19
            Pseudo Genes (internal stop)      :: 5 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18283
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3662_length_18283_cov_7.014922"
                     /isolate="AMR_MDS_3064"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(43..234)
                     /locus_tag="P5123_03315"
     CDS             complement(43..234)
                     /locus_tag="P5123_03315"
                     /inference="COORDINATES: protein motif:HMM:NF016629.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rpn family recombination-promoting
                     nuclease/putative transposase"
                     /protein_id="HRX15320.1"
                     /translation="MNEHDKLFKEIESVKENAADLICSTFPVDILAELDISTLILDNN
                     CYVDDNLNEHFADLVYFRF"
     gene            427..714
                     /locus_tag="P5123_03320"
     CDS             427..714
                     /locus_tag="P5123_03320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012805666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0110001 - toxin-antitoxin complex
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system Phd/YefM family
                     antitoxin"
                     /protein_id="HRX15321.1"
                     /translation="MNISKDIRPISYIKSHATDILKQINESRRPVYITQNGEARAVLV
                     DSNSYESMQKTIMLLKLVSQAEKDIIDGKYNDHDSFFTDLEKELEITFNSK"
     gene            711..1037
                     /locus_tag="P5123_03325"
     CDS             711..1037
                     /locus_tag="P5123_03325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013134705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008657 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) inhibitor activity
                     [Evidence IEA];
                     GO_function: GO:0042803 - protein homodimerization
                     activity [Evidence IEA];
                     GO_process: GO:0009432 - SOS response [Evidence IEA];
                     GO_process: GO:0030541 - plasmid partitioning [Evidence
                     IEA];
                     GO_process: GO:0051290 - protein heterotetramerization
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HRX15322.1"
                     /translation="MKNYEVQWTSTAQNDVRNIFSFIINDGISRSKQLLNQLRDRGNK
                     LKTNPEQGRIVPELAENNIYTYREVFHKPWRIIYRIENNRVYIVSVIDGRRNIEDILL
                     RRILEI"
     gene            1224..2921
                     /gene="recN"
                     /locus_tag="P5123_03330"
     CDS             1224..2921
                     /gene="recN"
                     /locus_tag="P5123_03330"
                     /inference="COORDINATES: protein motif:HMM:TIGR00634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RecN"
                     /protein_id="HRX15323.1"
                     /translation="MLQKLHIKNFVLIEELTMEFSEGLNILTGETGAGKSILIDAISA
                     ILGGKVTVSSIRTGCDRAILEGTFDITTLPLLKEILDNSGIDTEDDFLVIKREIYSGG
                     KGRCFANGTQIPIALLKDISDYLIVIHGQNEHVSMTKTSTHRELLDSYACNGELLDAV
                     KTYYNELHDLKEKISSTEMDEQERNKRIDYLTFALNEIEEANLSLNEEEELKDESSLL
                     SHAEKLCSEVAESLDALKGDQGVLRKLESIDSSLSSVSQIDPNIASELENIRTALYQL
                     EDSAAFFRGYSDSIDFSPERLNEIEERLALISGLKKKYGKSTDEILSFADNCRNELDM
                     ISNYDYEQEKLKEIYRETVTKTRDTALKLSRSRQKAAQELEQKVISELNDLNMKGAQF
                     KILVEQETSGSGEIEVGGKTYMLYPHGLDKIEFMLAANRGEALTQLRKAASGGEMSRI
                     MLAIKKVLLDADIVDSLIFDEVDTGISGQTADIVGRKLHSLAERRQVFVITHLAQIAA
                     MSDSHYIVQKQEKDGRTRTTVNKLSIKEKIREVARMLAGNNITETTLLQAQELIENR"
     gene            complement(2923..6168)
                     /locus_tag="P5123_03335"
     CDS             complement(2923..6168)
                     /locus_tag="P5123_03335"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HEAT repeat domain-containing protein"
                     /protein_id="HRX15324.1"
                     /translation="MEKDFLQSIKLEISRNFSLTAYERIAFHKILNIYKSDNGAEILL
                     RDIGKPGAIRLSALEVISGFNYKDAFPVIKEILNTSEKKEELCLVFDFIENNGTAYFL
                     SDLENFAKKRGANTFPAEINRAVFLTGLLVKRIDPEEDFSGADYGLPDKDEEDQPIVV
                     KGKELVSRYTEMLIGIAQNGENPVRLRATAITALDHYTELPFYQKMLEEDNDTISFAV
                     FQTVAQFAHREMKIYQDLNQKDVFTHVPPKEDNFMLNVRVLLSTFSNRFDSFSNETKV
                     AFIKVLLACNHREYLVYIMKTLTSGDNNLIDMTLYTILAAIDYLNNPDKLFRNLIAIP
                     SLTDRDNQIIVETFVKYFSKSQRNQSSNLIRDKLYNYIIVMLDNFFKSYCDNYIVPEI
                     KEKDYIDEFQAIRKFVLEKCDPFLKRRIIKFLTTEDIDLKTLLIEIAERITFVNESEE
                     KALALLLEVLHEPDAKAREISAQRLNDIDFEKKYLKLRLIRLCHIIGELRIKDASNSL
                     GKMFDYIKKFYDEELFCAITYSLSQLSYPYMLGELEYLFLNSDSDDEALKMLHYLSQF
                     SEQRATIIMLDFLKSRFGDVSPLILRVLQVIAKKDIKSDKSVNEFSKIIVDKNRNSEI
                     RASAVHLIGKTGFERDVLYLHELFMEENDNIVKEAVVQALDFIGIINPTIRKKEIAPF
                     LKGYLKDSSIKVRIYSCIILLQSGDLGALSLLKDMMIIRNKDIQREILLVLGSYMTIE
                     LAFFLLSLFNSDYGITRDIVPLFNYLSPEDKKEIDHFIVNIFKKHEGTRVDFAEKSIL
                     ESNQKYNEQLSTFVSDARPVMYVVVKDYHTILTRYRSTELSMLVKNSLMRLVDVIKAN
                     DGVVVQVTNGCIDSYFKTLPDSAMAALEVQALEKDINFTVPEDEHLEFFIYTEHADLC
                     LTGNEILLNSVSNLKTALSSGLKNRVFLNRKTADGINMAFKCETYPETPFDPRGREFG
                     FRELLNALNFNALVDEMLARIRREMNDRIQRKKEVDRTSVKHKVYLSKEAQQISDSIN
                     ELGKVLKKDLSDILQYVKKRSTDRELIKQVDGMLADAYKRFTIEKNKINLE"
     gene            complement(6200..6631)
                     /locus_tag="P5123_03340"
     CDS             complement(6200..6631)
                     /locus_tag="P5123_03340"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRX15325.1"
                     /translation="MRKKILFCGLMFSLLLNAAEIIELTNSEFTDSISNKSSLLRVQL
                     LESFVNKTVLGEGVVESVESVDAKEGYNSKKIVTIRHKAANSNLLIIFRLFSSNDLSS
                     IELVGKRVQFKGKLVLTVPLDSTRTIFACDILLEELSIYAD"
     gene            complement(6675..7934)
                     /locus_tag="P5123_03345"
     CDS             complement(6675..7934)
                     /locus_tag="P5123_03345"
                     /inference="COORDINATES: protein motif:HMM:NF012318.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trypsin-like peptidase domain-containing
                     protein"
                     /protein_id="HRX15326.1"
                     /translation="MKWIMLKITLFFTFAVIIAAESPGFSDAVSRSLPSVIYIESYLI
                     SNGKKKQSGFGSGTVFSDRGHIITNHHVVKNADYYEAVMYDGTRCTFKKYPGNRYYIS
                     DSETDLALMRLDCKNYLYKPIEKEKDNNLKKGDWVIAIGNPYGLSNSVTKGIVSSTNR
                     SDVGFTNIEDFIQSDVPINPGNSGGPLINTSGKMVGLNTAIRTVSGGFQGISFSIPVN
                     IVESVYTDLLRFGHVRRGWIGVMVTEKSLAATGRKAVTVVSTLENSPAQLIGLQKGDI
                     IAEVDNNTIESKSELTKIVKNKPIGARVDLCVFRSGILKRFSFTMNEKDYFAKVGEAI
                     NTLYSVYGIRLDINASNDKIIIADMSRFRYSENKLLQGDVITALNGQSVENLSDFASV
                     LRRYKYKISFLEVNRNEKKIIIRLKNY"
     gene            complement(8005..8823)
                     /locus_tag="P5123_03350"
     CDS             complement(8005..8823)
                     /locus_tag="P5123_03350"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRX15327.1"
                     /translation="MVKSLSKLLAVSLIIATAISIFAQEGGGGAGAPAGVDEYALDPA
                     SEYSTADRPEDNYLARFHALDKIERLQKANLDKIFVLNVIKENFKDKGWDGDYNKVYE
                     GYKRAMDLYYRRQPIYSAVKLEENKKHINQLYQKVSDEYKKDTIEMLNLCATHILELT
                     LQANTASNPNSNRRLFDQILRLRVAYSEVDNAEEWRVKRQYHSSVLHYRIAKKYAIAI
                     LEDVKPDEYIGKYQVHKADNKNRVLEGETKASEVIDRKEDIKKDIDESSRPESY"
     gene            complement(9139..10467)
                     /locus_tag="P5123_03355"
     CDS             complement(9139..10467)
                     /locus_tag="P5123_03355"
                     /inference="COORDINATES: protein motif:HMM:NF025042.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerate kinase"
                     /protein_id="HRX15328.1"
                     /translation="MSKADNIIHEIYRAAIDAVNPYRACMEKISFSDGLLKIRGVSRT
                     YRIEDYESVYIIGAGKATASMALALEKILGDYISEGVISVKYGYTEKLDRITQIEAAH
                     PVPDENGITAAKKVYDIAVKAGENDLIISLISGGGSALLPLPVEGISLDDKNIVTEAL
                     LSGGASIDQINCIRKHISIVKGGKLAKAAYPATVLNLMISDVPGDCIDVIASGPFTLD
                     RSTFAEARQILEGLPANCEIPESVRRHINEGCEGNIRENPDEKAAFFANVTNCVLLSN
                     FTALDFAASIARNAGYNTLILSSSIEGNVGEAVGFHIAVVKEIQATGNPVKPPACIIS
                     GGESFVRVEGSGKGGRNTEFVLRALTQMDGMKNIWAASIATDGTDGLTDAAGAVINYK
                     SYIKMLERGLQPEKFLKNSDSYSFFEQSGDLVKTGPTNTNVMDIHIFIVE"
     gene            10720..11856
                     /locus_tag="P5123_03360"
     CDS             10720..11856
                     /locus_tag="P5123_03360"
                     /inference="COORDINATES: protein motif:HMM:NF023462.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3524 domain-containing protein"
                     /protein_id="HRX15329.1"
                     /translation="MNNKKLHILAVEPLYTGSHRDWLDNLVTHSRHRFDLITMPAGFW
                     KWRIQGGALYLADQIKTDSYDLILTTSMLNTSLFLSLNRHNDIAKTRTAVYFHENQLN
                     YYPDDSKNQSAIKNDYSYAMISITNLIIADTVFFNSNYHRDIFFEQLPRFMSKLPDYN
                     SLPFKELKSKCKILPVGIKTELNDSNLTEVSTSADPEVNNNKIPVILWNHRRDADKNP
                     DGFLSFLKIIDNKNCKFKLILLGAENKSDKVFNQIEQLFKDRIIFNGFASTKTEYTRL
                     LKQAHFAPITSDHEYFGISVMEAAYNGVIPLLPSSLVYPTLYHPFNELFYTPHDYLAL
                     AELFIKLSKSDNYLLKKNLHNHALKYSWNFLIKKYDDYLLQASI"
     gene            12123..13016
                     /locus_tag="P5123_03365"
     CDS             12123..13016
                     /locus_tag="P5123_03365"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HRX15330.1"
                     /translation="MCNIKGILVIPDKLNDIYAFDSIISLEKEIYAKSIHTLDISSVS
                     FIEPYSMTNLLLIARRYLKNTGNRLKLTQIPLPIHQYLTRMNFFKYPIFDEPEPLDSK
                     KMLTRSALSKRILEITEIPNLERESVRVISQAVSLFRRRGAYILKHWFCPDTVNYFVT
                     VISEVCQNVFEHSMDSGFLALQTYTYNRENVVRLVIADSGIGIEESFSAEQKKTYGTG
                     SALIKNALTAPISGKREFGYGLCRVNSIIKEMKGNLFIRSDRSSVAFVYNKGYSDNAY
                     SFLKDDLEPFSGTQISISLYG"
     gene            13020..13568
                     /locus_tag="P5123_03370"
     CDS             13020..13568
                     /locus_tag="P5123_03370"
                     /inference="COORDINATES: protein motif:HMM:NF025574.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="STAS-like domain-containing protein"
                     /protein_id="HRX15331.1"
                     /translation="MIISVKKIAKALKSDSPDLLTRPTGRRFFEQAIKKLENASTDET
                     VICDFSGIEVIDPSFVDEFIVKLIKTSQDDKKPFYIRLKNISSVVEENIRSVFTSYCE
                     FAKTKFSVITEDITKDNTHIIGITSDTEREIIEIMRINGTMATETISKHLGLDSIDTA
                     HILEQMHKDRLVKKDSDRYLVV"
     gene            13569..14036
                     /locus_tag="P5123_03375"
     CDS             13569..14036
                     /locus_tag="P5123_03375"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRX15332.1"
                     /translation="MNKDYTYTFDKNRESLHDLLTAYKQFNTYFWIMVNREKKTLMFI
                     MASNPRHFRLIPEEETAGFRPMKRVELSVNKMYTAYISPSHLLAAYGPTLGINFPQKT
                     ITVQNKLKTPKHNYYLIKAPIRYLPDIIQAVKGILDLEPQIKYEYVEKYLLEH"
     gene            14217..15200
                     /locus_tag="P5123_03380"
     CDS             14217..15200
                     /locus_tag="P5123_03380"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRX15333.1"
                     /translation="MALEDTNRMVLALKLAGNDADKARRLVSGELKDVRVVYGRFSIE
                     AADVFGVFELFFHLQDENLLHVSLVTFSDPQEYEKTKLTDNWKLYYTILKQLQETVSV
                     TESEDFLKQLTDSIDGYQLIYDVVDNDEERITDTLVEIIEKSYGLPGAECSVDFADLS
                     TLEMADTGISLKLASDKKTEVKQDKPEIEKLAAHTVEGALVIAPVKGTYINDIRIGDI
                     VKVSLINHDDVSKKIITALNAFDSENNFLPIRGRVKEIIPLPTGGYKIYCLIAKNILA
                     RIVEEENIKAEMWDFSKITKGKNDRSFKNLGVIFALTLIVIASLAVLAALL"
     gene            15207..16169
                     /locus_tag="P5123_03385"
     CDS             15207..16169
                     /locus_tag="P5123_03385"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRX15334.1"
                     /translation="MRFISIRFFLCLAAVIFLQSQMPGWLSITDNANNHYYVTPTGKI
                     ITNETPDFTYKPVSFEGIDYYLQQGEILSQKFNKFEGLLLLKSIRYLGDIDERIMFES
                     ARAASLITKLKNREGDRFKKLNSNASILLYSFNKQTTLFNDFSNYRINLNDAQIFILN
                     RAVRDDEFSSNDSFSAGLKFSATGDDSTSEYDAVIFASVIKNRYSKYKSVEHFRKHLV
                     QRRGSDSFERDTIKKDENYLLSSFTFTKTGTKENKLLKDTPSFNGKEALSAEDSGKKQ
                     FVGYEVLTCGKNSGLLLQIMVSGENGAAYDNKIKDIALSLEATL"
     gene            complement(16159..16953)
                     /locus_tag="P5123_03390"
     CDS             complement(16159..16953)
                     /locus_tag="P5123_03390"
                     /inference="COORDINATES: protein motif:HMM:NF015114.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRX15335.1"
                     /translation="MRAKIVFVLLVFFSPLYAESPMFRQLMEGTLEQKTQAMYHLGYA
                     KSRKPFWLYVKYLSFMPQERDEMSAIQCREAAADALGRIKDPRAAKYLLERWEVEKQP
                     IVKRKIIFSLRNYRDEKIADVIRAGLKDEDERVVFESVVSSAFYKNESLSTDIKSFMD
                     KTEDTALKAACAFSLIMLNYEVTDNAKFLRESLTNKNPEVRYWCAFFLGEADRVEAIR
                     DLERALDIEYRYYVARQIDLAIIRLRFEIKANRDLREYNEHEHIIK"
     gene            17303..18172
                     /gene="metF"
                     /locus_tag="P5123_03395"
     CDS             17303..18172
                     /gene="metF"
                     /locus_tag="P5123_03395"
                     /EC_number="1.5.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011889947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004489 - methylenetetrahydrofolate
                     reductase (NAD(P)H) activity [Evidence IEA];
                     GO_process: GO:0009086 - methionine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylenetetrahydrofolate reductase [NAD(P)H]"
                     /protein_id="HRX15336.1"
                     /translation="MHINKLLNKNRPSFSFEFFPPKTDAGFEKLYSTIKDLTPLNPTF
                     VSITYGAGGSTRQKTHDLVMKLHKETELTVIPHLTCIGSTMNEIDEIVSDYHDNGISN
                     ILALRGDPPKDTENFVKTKDGFEHAADLVSFIKKNYPNMGIGVAGFPEGHPLTPNRLL
                     EMDYLKQKVDAGADYICTQLFFHNDDLYDFIERCELSGIDIPVLAGIMPITTISGMKR
                     MAELSAGSRFPAKLLKQIYASSSDNEIKQIGINWATNQVKDLVKNDIPGVHFYTLNNS
                     EATLLIYRGLSSD"
BASE COUNT         5998 a         3725 c         3005 g         5555 t
ORIGIN      
        1 attaattcat cgtcgtagcg aaaaatatca gtcaaaatat aactaaaatc tgaaataaac
       61 cagatcggca aaatgctcat taagattatc atcaacataa caattattat cgagaattaa
      121 tgtgctgata tccaactctg ccaagatatc caccggaaat gtagagcaaa tcaaatctgc
      181 agcattctct ttaacgctct ctatctcttt aaagagctta tcgtgttcat tcatctttca
      241 cctcttttcc cttacatata ccaacttatt ttcagagaaa attgacgaaa aaaataatat
      301 gtttggaaaa aaattttaca attctgctta tttcctgtta ttaatgctat aagctatcat
      361 aaaattgtta ttgaccaacc taaccaaatt gagtactata tttagactct ttacagtgga
      421 gatgaaatga atatttctaa agacatcaga cctatttctt atataaaatc acacgcaacc
      481 gatatattaa aacagataaa cgaatctcgc cgccctgtgt atattacaca aaatggagaa
      541 gccagagcag ttcttgttga ttctaacagc tatgaatcga tgcaaaaaac aatcatgttg
      601 ttaaaactcg tttctcaagc tgaaaaggat attattgatg gcaaatacaa tgatcacgac
      661 tcctttttta ccgatctgga aaaggaactg gagattacgt ttaatagtaa atgaaaaact
      721 atgaggttca atggacgagt actgctcaaa atgatgttcg caatattttc tcttttataa
      781 ttaatgacgg tatttctcgc agcaaacaac tattaaatca acttagagat cgtggaaaca
      841 aactaaaaac aaatcccgag cagggaagaa tcgttcccga attagctgaa aacaatattt
      901 atacataccg agaggtattc cataaaccgt ggcggatcat ttacagaata gaaaataatc
      961 gagtatatat agtttccgtt atcgatggca gaagaaatat tgaagatatt ctattacgta
     1021 gaattttaga aatataatgg ttcaaaatta ttcaaacaga gaatcattta ttaacaaaaa
     1081 aataataatt ctgcttattt cccggtatta atgctataag ctgtcataaa attttggttg
     1141 aacattcttc cgcggccttt aatctgactc tgacggatag ccatgtaatc agaccgtgaa
     1201 agtctttaaa caagcaggtt attatgcttc agaaactaca cattaaaaat ttcgtactta
     1261 ttgaagagct caccatggaa tttagcgagg ggctcaatat tcttaccgga gaaaccggtg
     1321 ccggtaaatc gatactcatt gacgcaatat cggcaattct gggcggcaag gtaactgtca
     1381 gctcaatccg tacagggtgt gaccgtgcca ttcttgaagg aacatttgat ataacaacac
     1441 tacctcttct aaaagagatt cttgataatt cgggtatcga caccgaagac gattttctgg
     1501 taattaaacg cgagatctat tccggtggaa aaggacgctg ttttgcaaac gggacgcaga
     1561 taccgatagc tctcctgaaa gatatctccg actatctgat agtcatccac ggacaaaacg
     1621 aacatgtctc tatgacaaaa acatcgacac acagagaact tctggatagt tatgcctgca
     1681 atggagagct ccttgacgca gtcaaaacat attataacga gctccacgac cttaaagaaa
     1741 aaatatcatc aaccgaaatg gatgagcaag agcgtaacaa acgcatcgat tatctgacat
     1801 tcgctctaaa cgaaatcgaa gaggccaacc tctcattaaa cgaagaagaa gagctcaaag
     1861 acgaatcctc gcttctctct cacgccgaaa aactctgctc agaggttgcc gaatcacttg
     1921 acgccctaaa aggtgatcaa ggagttttac gaaaactcga atcaatcgat agctctctaa
     1981 gctcggtatc acaaattgat cccaatatcg cttccgagct cgaaaacata cgcaccgcgt
     2041 tataccagct tgaagactcc gcggcatttt ttagaggcta cagcgattct atagattttt
     2101 caccggaaag gctaaacgaa attgaagaac ggcttgcact aatatctggg cttaaaaaga
     2161 aatatggaaa atcgactgac gaaatactct ctttcgccga caattgtcgt aacgaactgg
     2221 acatgataag caattacgat tacgaacagg aaaagttaaa agagatttac cgtgagacag
     2281 taaccaaaac acgagataca gcgcttaaac tttcccgatc ccgtcaaaaa gcagcacaag
     2341 agcttgaaca aaaagttatt tccgaactta acgaccttaa catgaagggt gcccagttta
     2401 agattcttgt cgaacaagaa acatctggta gcggagagat tgaggttggt ggaaaaactt
     2461 atatgctata tcctcatgga ctagacaaaa tagagtttat gcttgccgca aatagaggcg
     2521 aggcactaac ccagctgcgc aaggccgcat cgggtggaga gatgtctcga ataatgttgg
     2581 cgataaagaa ggttctgctt gatgctgata ttgttgactc tcttattttc gatgaagtgg
     2641 ataccggaat aagtggtcaa acagccgata tagtcggtcg caaactgcac tcacttgccg
     2701 aacgacgtca ggtttttgtg attacacacc tggctcaaat agcagcaatg agcgactcac
     2761 actatattgt gcaaaaacag gaaaaagatg gtcgaactcg tacaacggta aacaagctct
     2821 ctataaaaga aaagatcaga gaagtagcac gaatgcttgc aggtaataat atcaccgaaa
     2881 ctactcttct ccaggctcaa gagctgatcg aaaaccgcta gcttattcca gatttatttt
     2941 gtttttttca attgtaaatc ttttataagc atccgcgagc ataccatcta cctgtttaat
     3001 aagttctcgg tcggttgatc ttttttttac atattgtagt atatccgaca agtccttttt
     3061 taacacttta cccaattcat ttattgagtc cgaaatctgt tgagcttcct ttgaaagata
     3121 aaccttatgt tttaccgaag tgcgatctac ttcttttttg cgttgtatac gatcattcat
     3181 ctcccgcctt atacgggcta gcatttcatc gacaagagca ttaaaattaa gcgcatttaa
     3241 cagttcacgg aatccaaact cgcgtccgcg gggatcaaaa ggagtctctg gatatgtttc
     3301 acatttaaaa gccatgttta ttccatcagc tgttttacga ttaaggaaaa ccctgttttt
     3361 tagaccacta gataacgcag tttttaaatt cgaaacacta ttaagtaaaa tctcgtttcc
     3421 tgttaaacat aaatctgcgt gttctgtata aataaaaaat tctagatgct catcctcagg
     3481 aactgtgaaa ttaatatcct tttcaagcgc ctgaacttct aatgcggcca ttgcactatc
     3541 aggaagcgtc ttgaagtacg aatcgataca accgtttgta acctgcacaa caaccccgtc
     3601 atttgccttg ataacgtcga ccaaccgcat taatgaattt tttacaagca tggacaattc
     3661 tgttgaacga tatcttgtta aaattgtatg ataatctttg acaacaacat acataaccgg
     3721 tcgcgcatca ctaacaaagg ttgacaactg ttcgttatac ttttgatttg attcaagtat
     3781 cgacttttcc gcgaaatcga cccttgttcc ctcatgcttc ttgaaaatat ttacaataaa
     3841 atgatcaatc tcttttttat cttcaggaga cagataatta aatagtggca caatatcacg
     3901 tgttattcca taatccgagt taaaaagcga cagcaagaaa aacgccaact caattgtcat
     3961 ataacttccc agaacaagaa gtatctctcg ctgtatatct ttatttctaa ttatcatcat
     4021 atccttaagc aaagaaaggg ctcccaaatc acccgattgc aaaagaataa tacatgaata
     4081 tattctgact tttattgaag agtctttcaa ataacctttt aaaaatggtg ctatctcttt
     4141 ttttcggata gttgggttaa taatcccgat aaaatccaga gcctgaacaa ctgcctcttt
     4201 tacaatatta tcattctctt ccatgaatag ctcatgcaaa taaagtacat cccgctcaaa
     4261 accggtctta ccaatcaaat gaaccgcaga agctctaatc tcagaattgc gatttttatc
     4321 cacgattatc ttagaaaatt cattaacaga tttatcactc ttaatatctt tttttgcaat
     4381 tacctgaagc acacgcaaaa ttagcggtga cacatctcca aaacggctct tcaaaaaatc
     4441 gagcataata attgttgctc tctgttcaga aaattgcgaa agatagtgca gcatctttaa
     4501 tgcttcatca tctgaatcac tattaagaaa aagatactcc agctcgccaa gcatgtaagg
     4561 ataacttaac tgtgataggc tgtatgtaat cgcgcaaaac aactcttcgt cgtaaaactt
     4621 ttttatgtaa tcaaacattt tgccaagcga attagaggca tctttaattc ttagctctcc
     4681 gattatatga cataacctta tcaatcttaa ctttaaatat tttttctcga aatcaatatc
     4741 gttaaggcgt tgtgcgctaa tctctcgagc ttttgcgtct ggctcgtgca acacttcaag
     4801 aagcaaagct aatgcttttt cttcactttc attaacaaat gttattctct cggctatttc
     4861 tataagtaaa gtctttaaat cgatatcttc tgtagtcaga aattttatta tacgccgttt
     4921 taaaaacgga tcacattttt ccagaacaaa ttttctgatt gcctgaaatt catcgatata
     4981 atctttttct tttatttccg gtacaatata gttatcacag taactcttga aaaaattgtc
     5041 caacattaca attatgtaat tataaagttt atctcgtata aggttcgagc tttgatttct
     5101 ttgtgattta gaaaaatatt taacaaaagt ttcaacaatt atctgattgt ctctgtctgt
     5161 taatgacggt atggcaataa gattacgaaa cagcttatca ggattattta aataatctat
     5221 agcggcgaga atagtataca aggtcatatc tataagatta ttatcacctg aagtcaatgt
     5281 cttcatgata tataccaagt attcacggtg attgcaggcc agaagcactt ttataaaagc
     5341 cacttttgtt tcgtttgaaa acgagtcaaa gcgattacta aacgtgctta gcaaaactcg
     5401 cacattgagc ataaagttat cttctttagg aggaacatgt gtaaatacat ctttttgatt
     5461 aagatcctga taaatcttca tttcacgatg tgcaaactgc gcgacggtct gaaataccgc
     5521 gaaagagatt gtgtcattat cttcttcaag cattttttga taaaacggca attcagtata
     5581 atggtcaagt gccgtgattg ctgttgcgcg aagcctcact gggttttccc cgttttgagc
     5641 tatacctata agcatttctg tataacgtga aacaagctct ttgcccttaa caactattgg
     5701 ctgatcttcc tcatccttat ccggtaatcc ataatccgca ccactaaaat cttcttcagg
     5761 gtctattcgc tttacaagca agcctgttaa aaatacagcc ctatttatct cggccggaaa
     5821 tgtattagcg cctctttttt ttgcaaaatt ttcaagatca gataaaaaat atgctgttcc
     5881 attattttca ataaaatcaa aaaccagaca taattcctct tttttttcac ttgtatttaa
     5941 gatctcttta ataacaggaa aagcatcttt ataattaaag cccgaaataa cctcaagagc
     6001 gctaagtctt atagcacccg gttttcctat atctcttaaa agaatctcgg caccattatc
     6061 gctcttgtat atattaagaa ttttatgaaa cgctatccgc tcatatgctg ttaaagaaaa
     6121 attgcggcta atttcaagtt ttattgattg aagaaaatct ttttccattg aatcctctta
     6181 aaacatggat tttacagatt taatccgcgt aaatcgaaag ttcttcaagc agaatatcac
     6241 aagcaaaaat cgttctggta ctgtcaagtg gtaccgttag aacaagcttt cctttaaatt
     6301 gcactctttt cccaacaagc tcaattgatg ataaatcatt tgaagaaaaa agtctgaaaa
     6361 taattaacag atttgaattt gcagctttat gtcttatcgt tactattttc ttgctgttat
     6421 aaccttcttt cgcgtctact gactctactg attctacaac accttcacct aaaacagtct
     6481 tattaacaaa agattcaaga agttgcactc ttaatagaga agatttgttt gatatactat
     6541 cagtaaattc actgtttgtc agctcgatta tttctgctgc gttcagcaat aaagagaaca
     6601 ttaaaccgca aaataatatt ttttttcgca ttctaaatcc tgtttcttct ttcccgatga
     6661 tttggttata tcttctaata atttttcagt ctgataatta tttttttttc attcctgtta
     6721 acttcaagaa atgaaatttt gtatttatat cgtcttaata cagaagcaaa atcactcagg
     6781 ttctcaacac tttggccatt caatgcagta ataacatctc cctgcaacaa cttgttttcc
     6841 gagtatctaa atcgcgacat atccgctata ataattttat cgttagacgc gttaatatca
     6901 agacgtattc cgtaaacaga gtacaaggtg tttattgctt ctccgacttt ggcgaaataa
     6961 tctttttcat tcattgtaaa tgaaaatctt tttaaaatac cgcttctaaa gacacataga
     7021 tccacacgtg cacctatcgg tttatttttt acaattttgg ttagctcact tttagattca
     7081 atagtattgt tatccacttc ggcaataata tcaccttttt gcaaaccgat aagctgtgct
     7141 ggtgaatttt caagtgtcga tacaacggtt accgccttac gtcctgttgc agctaagctc
     7201 ttttcggtaa ccatgacccc tatccatccc cgtctaacat gtccaaaacg caacaaatcc
     7261 gtatatactg actcgactat attcacgggt atcgaaaacg atattccctg gaaaccacct
     7321 gatacggtac gtatagcagt attaagacct accatctttc cagatgtatt aatcaatggg
     7381 cccccagagt tccccggatt tattggaaca tctgactgaa taaaatcttc tatatttgtg
     7441 aatcccacat ctgatcggtt tgtagatgag acaattcctt tagtaacact atttgataag
     7501 ccatagggat ttccgatggc aataacccaa tcaccctttt ttaaattgtt gtctttttcc
     7561 ttttcaattg gcttgtacaa gtaattcttg caatcaaggc gcatcaaggc aagatctgtt
     7621 tccgaatctg atatatagta tctattaccc ggatattttt taaatgtaca ccgagttcca
     7681 tcatacatta ccgcttcata ataatccgca ttttttacaa catgatgatt agtaattata
     7741 tgccctcgat cagaaaaaac tgtccctgac ccaaaacccg actgtttttt tttaccatta
     7801 gaaattaaat aggattcaat atatataacg gagggcaaag atctgctaac ggcatcggaa
     7861 aaacccgggc tttcggccgc gatgattaca gcaaaagtga aaaagagagt tatcttaagc
     7921 attatccatt tcattacttt gacctgataa aaaaataagg aagggagaac ccttccttat
     7981 tttcggctaa aaatattcta tctattagta agattctgga cgtgaactct catctatatc
     8041 ttttttaata tcttctttac gatcgattac ttcggaagcc ttggtttcac cttcaaggac
     8101 gcgatttttg ttatctgcct tatgcacctg atatttgcca atatactcat caggtttaac
     8161 atcttctaga atagctatcg cgtatttttt tgctatacga tagtgaagaa ccgatgagtg
     8221 atactgacgc ttaacacgcc actcttcagc attatccact tcgctgtaag caacacgcaa
     8281 tctaagaatc tggtcaaata gtcgtctgtt gctgttaggg ttagaagctg tatttgcctg
     8341 taaggttaac tcaagaatat gagtagcaca aagatttaac atctcaatag tatctttttt
     8401 atactcgtct gataccttct gataaagctg attaatatgt ttcttgttct cttcaagctt
     8461 tactgcagaa taaataggct gacgtctgta ataaagatcc atcgcccttt tataaccttc
     8521 gtaaaccttg ttgtaatctc cgtcccaacc cttatcctta aaattctctt ttataacatt
     8581 aaggacaaat attttgtcga gatttgcttt ttgaagtctt tcgattttat ccagagcgtg
     8641 aaaacgcgca agataattgt cctcaggtct gtccgcagta gagtactcgg acgccggatc
     8701 gagcgcatat tcgtcgacac ctgccggagc accggcaccg ccgccgcctt cctgtgcaaa
     8761 aatgcttatt gctgttgcaa taattaatga aacggccaat aatttcgata atgatttaac
     8821 catgatctta actcctgtat actattatat atgctgtacg gcgtccttgc cagaatatac
     8881 ttgtgttgag tataattaat gaatttattt caggcaagaa tttttttact tttgaaacat
     8941 taattcattt aatttcgccc ctttgtgccg tgtttgttaa ataaatcata ctacttaagc
     9001 cttttcaaag cgtaatacga cataacaaac agagctatca aaaagccgta taaccggcta
     9061 tatacataat cgaaaaataa gtgcacatac cttaattaat aagtgaaaat tatttaaact
     9121 ttcgtgaaaa ccgcaaaatc actctacaat aaaaatgtgt atatccataa cattggtatt
     9181 ggtagggcca gtctttacaa gatcaccact ctgttcaaaa aacgaataag aatcactgtt
     9241 ttttaaaaac ttctcaggct gcaaacccct ctccaacatc ttaatataac tcttataatt
     9301 aattaccgcc ccagcggcat cagttaaccc atctgttcca tctgttgcta tgctggcagc
     9361 ccatatattc ttcattccat ccatctgagt cagagcccta agcacgaatt cggtattccg
     9421 accacctttg ccactccctt ctactctaac aaaactttct ccaccgctta tgatgcaagc
     9481 cggtggctta acgggatttc cggttgcctg tatctctttt accaccgcta tatgaaaccc
     9541 aactgcttcc cctacgtttc cttcaattga tgaactcaaa atcaaggtat tataccctgc
     9601 attacgggca atactcgctg caaaatcaag agctgtaaaa ttggaaagca aaacacaatt
     9661 agtaacattt gcgaaaaaag cggccttctc gtccgggttt tcccttatat ttccctcaca
     9721 accctcatta atatgtcttc gtacagattc tggaatctca caatttgcag gtaaaccttc
     9781 cagtatttgt cgtgcttctg caaaagttga tctgtccaga gtaaaaggtc cagaagcaat
     9841 aacatcaatg caatctccag gaacatccga aatcatcaga ttcaaaacgg tggcggggta
     9901 agccgcttta gccagttttc cacctttgac tatcgagata tgttttctta tacagttaat
     9961 ctggtcaata gaggctcctc ctgaaagaag tgcctctgta acgatgtttt tatcatccag
    10021 ggaaattcct tcaaccggaa gtggtaaaag cgccgaaccg ccccccgaaa taagtgaaat
    10081 aatcaggtca ttttccccgg ctttaacggc aatatcatac acctttttgg cagcggtaat
    10141 tccgttttca tcaggcacag gatgagccgc ctctatctgg gtaatacgat ccagtttctc
    10201 tgtatacccg tatttcacag aaatcacacc ttcgcttata tagtctccca atattttctc
    10261 aagagcaaga gccattgacg cagttgcctt gccagcccca attatgtaaa cactctcata
    10321 gtcctctata cgataggttc tgctcacacc ccggattttg agcaagccat cagaaaagga
    10381 tatcttttcc atacatgccc tgtatggatt cacagcatca atcgcagctc tataaatttc
    10441 atggatgatg ttatctgctt tgctcatttt taaaaaactc cacacattac gcatttactg
    10501 ccaaacggga tttatgtatt acgattatcc atattagtga atgaaaacca cttgatgcaa
    10561 acgttcataa attattacat gtcaatacaa tgatatgtta agacaaccat taacaatata
    10621 tcatgattat ccggagagat caacaaaaca cgacacagtt ttgttccaat tgtttatata
    10681 attatcctta attacaaaaa ctgttcggct aaatagaaaa tgaacaacaa aaaacttcat
    10741 atcctggcgg tagaacctct ctataccgga tcacaccgtg attggctgga caacctagtt
    10801 acacacagcc gtcaccggtt tgacctaata acaatgcccg ctggattctg gaaatggcga
    10861 atccagggag gagccctgta tctggcagat cagattaaaa cagacagtta tgatcttatt
    10921 cttacaactt caatgctcaa cacatcgctt tttctatctc ttaacaggca caacgatatt
    10981 gctaaaacca gaactgctgt ctactttcat gaaaatcagc taaattacta cccggatgat
    11041 tcaaaaaacc agtctgctat caaaaacgac tactcctacg caatgattag tataactaat
    11101 ttaatcatag cagatactgt cttctttaat tcaaactatc accgtgatat tttctttgaa
    11161 caacttcccc gttttatgtc taaacttcct gactacaact cgttaccgtt taaagaactc
    11221 aaatcaaaat gcaaaattct tccagtcgga ataaaaaccg aattaaatga ttcgaattta
    11281 acggaagtat cgacttcggc tgatccagaa gttaataata acaaaattcc agtaattctg
    11341 tggaaccacc gtcgtgatgc agacaaaaat cctgatggct ttttgtcttt tttgaaaatt
    11401 atcgataaca aaaattgtaa atttaaatta attttactcg gagcagaaaa taaatctgat
    11461 aaggttttta accaaattga gcaactattt aaagacagga taattttcaa cggcttcgcc
    11521 tcaaccaaga ccgaatatac aaggcttcta aaacaagctc attttgcgcc aataacatct
    11581 gatcatgaat actttggtat tagcgttatg gaggcagcat ataatggtgt tattccacta
    11641 ttgccctctt ctctggttta tccaacactc tatcaccctt ttaatgaact cttttatact
    11701 cctcacgact acctagctct tgcagaacta tttattaaac tatcaaaaag tgataactac
    11761 ctattgaaga agaatcttca caaccatgca ttaaaataca gctggaactt tcttataaaa
    11821 aaatatgatg attatctttt acaggcctct atctgaaagc ggtttaaagg tatttttact
    11881 accggatcta tttgtggtaa tctttgataa ttagtttttc aagctcatta ttaaacaatg
    11941 ccacttgcac gcttaagcct tcctgctgac aaaatttcct tctttaaaaa aaagaacaat
    12001 atgccgatta ttaacaaagg ttattcttga aaactgcgtt gttgttatgg tgctattgcc
    12061 tcgcaaaacg tatactggac agatgaaccg aaatattaaa accatactgg ctgttatcag
    12121 aaatgtgtaa tattaaggga attcttgtaa ttccggataa acttaacgac atctacgctt
    12181 ttgattcgat aatatctctt gaaaaagaga tatacgccaa aagtatacac actctggaca
    12241 taagctcagt tagttttatt gagccctact ctatgaccaa cttattatta attgcccgta
    12301 gatatcttaa aaacaccgga aatcgattaa agctgacaca gattcccttg ccgattcacc
    12361 agtatttgac gcgaatgaat tttttcaagt atcctatttt tgacgaacca gaaccattag
    12421 atagcaaaaa aatgttaacc aggagcgcgt taagcaaacg catactcgag ataaccgaga
    12481 ttccaaacct cgaaagagag agcgttaggg taatctcgca agcagtaagt ctttttcgaa
    12541 gacgtggcgc ttatatatta aagcactggt tctgccctga tacggtaaat tactttgtga
    12601 ccgtaatctc agaagtttgt cagaatgttt tcgaacatag tatggattcg ggattccttg
    12661 ccttacagac atacacttac aatagggaaa acgtggtaag gcttgtaatc gccgattccg
    12721 gtattggtat cgaagagagc ttttcagcag aacaaaaaaa aacatacgga accggctcag
    12781 cccttatcaa aaatgctctt accgcgccaa tatccgggaa aagagagttc ggatatggtt
    12841 tatgccgtgt aaattcgatt attaaggaga tgaagggaaa tctttttatc agatctgaca
    12901 ggagctccgt tgcctttgtt tataacaagg gttattcaga taacgcttac tcctttttaa
    12961 aagatgatct tgaacctttc agtggtacgc agatttccat ttcactttat ggttaagtta
    13021 tgataatttc ggtaaaaaaa atcgccaaag ccctaaaatc tgattccccc gatctgctta
    13081 cacgaccaac cgggcgaaga ttttttgagc aggctataaa aaagctggaa aacgccagta
    13141 cagacgagac agtgatctgt gatttttcag gcattgaggt gattgacccc tcttttgtgg
    13201 atgaatttat tgtcaaactt ataaaaacat ctcaagatga caaaaaaccc ttttatatca
    13261 gattaaaaaa tatatcttcg gttgtagagg aaaacataag atctgtattt acatcctatt
    13321 gtgaatttgc caaaactaaa ttttcagtaa ttactgaaga cataaccaaa gacaacaccc
    13381 atattatagg aataacttct gatacagaac gggaaatcat tgaaataatg agaataaatg
    13441 gcacaatggc aactgaaacg atatcaaaac atcttggact ggattctatt gacactgcgc
    13501 atattcttga acaaatgcat aaagatagac ttgtaaaaaa agacagcgac agatatttgg
    13561 tagtataaat gaataaagat tatacatata cttttgataa aaatcgcgaa tcacttcacg
    13621 atcttttgac ggcatataaa caatttaata cctatttctg gataatggta aatcgcgaga
    13681 aaaaaacact gatgtttatt atggcctcta accccagaca ttttagatta ataccagaag
    13741 aagaaacagc cggatttcga cccatgaaac gggtcgagct aagtgtaaac aaaatgtaca
    13801 ctgcatatat ttcaccgtct catcttctag cggcttacgg cccaacactg gggataaatt
    13861 ttccacaaaa aaccattacc gttcaaaata aacttaaaac acccaaacat aattattatt
    13921 taataaaagc gccaatcaga tatttacccg acataattca agctgttaag ggaatacttg
    13981 acctagaacc acaaataaaa tatgaatatg ttgaaaaata cttgcttgaa cactagagaa
    14041 cttcgaaata ttaccttttc aggaacggct ataccgttta aagcccatat ataaagcaat
    14101 ttttcgcaag ttccttatag aaaagtcaat tattagagat ttccactagt tttcagtgat
    14161 cctattgtcg attctaatat aacggaatgt aaaaagacag aacagaaaga ggaaatatgg
    14221 cgttagaaga tacaaacaga atggttctgg cactgaaact ggccggtaac gacgcagata
    14281 aggctcgtcg ccttgtatcg ggcgaattaa aggatgttcg cgttgtgtac ggccgttttt
    14341 ctatcgaggc agccgatgtt ttcggtgttt ttgaactttt ttttcacctc caggatgaaa
    14401 atttgttaca tgtttcactt gtaacctttt ctgaccccca ggaatacgaa aaaacaaaac
    14461 ttactgataa ctggaaatta tactatacca tattaaaaca gctacaagag acagtttctg
    14521 taacagaatc agaggatttt ttgaaacagc taacagactc aattgatggc taccaactta
    14581 tatatgatgt ggttgacaac gatgaagaga gaatcactga cacacttgta gaaatcatag
    14641 aaaagtctta cggactccct ggagcggaat gttctgtcga ttttgccgac ttgtcaacac
    14701 ttgaaatggc tgataccgga atatctctaa aacttgcatc agacaaaaag accgaagtaa
    14761 aacaggataa acccgaaata gagaaacttg ctgcccatac agttgaaggt gctctcgtaa
    14821 tcgctccagt aaaaggaact tacataaatg acattagaat aggggatatt gttaaggtat
    14881 cacttattaa tcacgatgat gtatcaaaaa aaattattac agctcttaat gcttttgatt
    14941 ccgaaaataa ttttttaccc atacgcggac gtgtaaaaga aataattcca ctgccaactg
    15001 gaggctacaa aatatactgt cttattgcaa aaaacattct tgcccgcata gtcgaagagg
    15061 aaaacataaa ggcagagatg tgggatttct caaaaataac caaaggcaaa aacgaccgaa
    15121 gtttcaaaaa ccttggtgta atatttgctt taacactaat agttattgcc tcattggccg
    15181 ttcttgccgc tcttttgtga ggatagatga gatttatttc aatccggttt tttttatgtt
    15241 tggccgcggt tatttttctt caatcacaga tgccaggatg gctctcaata accgataacg
    15301 ctaataatca ttactatgtc actcctaccg gaaaaattat cacaaacgaa acccccgatt
    15361 ttacttacaa gcctgtttca tttgagggca tcgattacta cctgcaacaa ggtgaaatac
    15421 tttcgcaaaa atttaataaa tttgaaggcc ttctgctttt aaaatcaatt agatatctag
    15481 gagatatcga tgagcgaata atgtttgaat cggcacgcgc ggcctcttta attaccaagc
    15541 tgaaaaaccg tgaaggtgac agattcaaaa aacttaacag taatgcatct attttattat
    15601 acagcttcaa taagcaaaca accctattca atgatttttc aaattacagg ataaatttaa
    15661 acgacgcgca gatatttatt ttaaacaggg ctgtacgtga tgacgagttc tcttctaacg
    15721 acagtttctc ggcaggatta aaattctctg caactggtga tgactctact tcagaatacg
    15781 atgctgtaat ctttgcatct gttattaaga acagatactc aaaatacaaa tcggtagagc
    15841 attttagaaa acatcttgtt caaagaagag gctcggacag ttttgaaaga gatacaatta
    15901 aaaaagatga aaattacctt ttatcttctt ttactttcac aaaaacggga acaaaagaaa
    15961 acaagctctt aaaggatacc ccatcattta atggaaaaga agccttatca gcagaagact
    16021 ctggtaaaaa acagtttgtc ggatacgaag tactgacctg tggcaaaaac agcggcctac
    16081 ttttacaaat tatggtatcc ggtgaaaatg gtgccgcgta tgacaacaaa ataaaagata
    16141 ttgccttgtc acttgaagct actttataat atgttcgtgc tcattatatt cacgaagatc
    16201 acgattagct tttatttcaa accttaatct tataatagcc aagtcaattt gacgggcaac
    16261 atagtaccgg tattctatat ctaaagccct ttctagatca cgaattgctt ctaccctgtc
    16321 agcttctccc agaaaaaaag cacaccagta cctaacctca ggattcttgt tcgttaatga
    16381 ttcacgcaaa aatttagcat tatcagtaac ttcataatta agcataatca aagaaaacgc
    16441 gcacgctgct ttcaaagctg tgtcttccgt tttatccata aacgatttaa tatccgtgct
    16501 taaactttca ttcttataaa aagccgagct gacaaccgat tcaaaaacaa ccctctcatc
    16561 ttcatctttt aaacctgcac ggataacatc ggctatcttt tcatctctgt aatttcgcaa
    16621 agaaaaaatt atctttcttt tgactatcgg ctgtttttca acctcccatc tttcaagaag
    16681 atatttggca gctcgaggat cctttatgcg cccaagtgcg tcagcagccg cttcacggca
    16741 ttgtattgcc gacatttcgt ccctctcctg aggcatgaaa ctaaggtatt ttacatatag
    16801 ccaaaacggc tttcgacttt tggcataacc caagtgatac atagcctggg ttttttgttc
    16861 aagagtcccc tccataagct gtctgaacat agggctctcc gcgtaaagag ggctaaaaaa
    16921 aaccagtaaa acaaacacta ttttcgcgcg catatctgct tcctccagac tattcaaaac
    16981 tatgataatc atatttcttt tcaactaata ttatcggatt ttatagctac gaatatgatt
    17041 tcatccagca tgtatttttt cttcgataca aaaaatattc acatccaaac atctgcaaaa
    17101 tttccgaaaa tcagtttttc tgcatgctaa cataattaga tataaacaaa acaatcgata
    17161 tagcaatcat aaaattcagt taagactact ttaaaaatca aaataggttg aagaaattag
    17221 gcatgatttt tagtatagaa aacttctagt aaataccaat tttgtaataa taatgaattt
    17281 ttatcaacaa aacggagcca ttatgcatat taataaactt ttgaataaaa accgaccatc
    17341 tttcagtttt gaattttttc ctccaaaaac agatgccgga tttgaaaaac tctattcaac
    17401 tataaaggat ctaactcctc ttaatcccac atttgtcagt attacttacg gcgctggtgg
    17461 ttctacacgt caaaaaaccc atgatcttgt aatgaaactc cataaagaaa cagaattaac
    17521 cgttattcca cacctgacat gtatcggctc aacaatgaat gagatcgacg aaatagtgtc
    17581 agactatcat gacaacggta tcagcaatat actcgcatta agaggtgatc cgcccaagga
    17641 taccgaaaac tttgtcaaaa ccaaagacgg ttttgaacat gcagccgatc tggtttcgtt
    17701 tataaaaaaa aattatccca acatgggtat cggcgtagcg ggatttcccg aaggacaccc
    17761 actaacaccc aaccgcttac tggagatgga ttaccttaaa caaaaagttg atgcgggtgc
    17821 cgactatatt tgtacacagc tattttttca caatgatgat ctttacgatt ttatagaacg
    17881 ctgtgaactg agtggaatag acattccagt acttgccggg attatgccga taaccacgat
    17941 cagcggcatg aaacgaatgg ccgaactgtc tgcgggatca cgctttcccg ctaaactgct
    18001 taaacagatt tatgccagca gttcggataa cgaaataaaa cagataggta ttaactgggc
    18061 aacaaaccag gtaaaagacc ttgtaaaaaa cgatattcct ggtgtacact tttatacgct
    18121 taataattca gaggccacac tgcttattta ccgaggttta tcatccgatt agtcaaacgt
    18181 agtacatttt gccctgtaaa attatagttt aaatcaaaaa acagaagaat cagccgtcct
    18241 caaggatttt ttactgttga aatcctcggc tgttaaagaa gat
//
LOCUS       DAOTGZ010000029        18141 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_3064
            NODE_3710_length_18141_cov_5.291994, whole genome shotgun sequence.
ACCESSION   DAOTGZ010000029 DAOTGZ010000000
VERSION     DAOTGZ010000029.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868088
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 18141)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 18141)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:22:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,785
            CDSs (total)                      :: 2,751
            Genes (coding)                    :: 2,732
            CDSs (with protein)               :: 2,732
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 12 of 19
            Pseudo Genes (internal stop)      :: 5 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18141
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3710_length_18141_cov_5.291994"
                     /isolate="AMR_MDS_3064"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            75..145
                     /locus_tag="P5123_03400"
     tRNA            75..145
                     /locus_tag="P5123_03400"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:107..109,aa:Gly,seq:tcc)
     gene            339..620
                     /locus_tag="P5123_03405"
     CDS             339..620
                     /locus_tag="P5123_03405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014803609.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRX15337.1"
                     /translation="MTKEKIFCANCRNCIVIRQHEAEPDKYVLRVKCKLRKWVKRSGE
                     EKLYKYFTVARRIMDDCDNYEESGELFPYIKNLRKELPIKDEIYSTKDQ"
     gene            624..1940
                     /gene="rsxC"
                     /locus_tag="P5123_03410"
     CDS             624..1940
                     /gene="rsxC"
                     /locus_tag="P5123_03410"
                     /inference="COORDINATES: protein motif:HMM:TIGR01945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="electron transport complex subunit RsxC"
                     /protein_id="HRX15338.1"
                     /translation="MFNPSFKGGVHPPENKKLTEERSIENLSVPHTCFVPVQQHIGKP
                     AIPVVQVGDYVQVGQLIAKADGFISSNIHASIPGKIASITNGPTPASPSGLRIQIETH
                     GNFTSEKTPDKIEWDKLPPKDLLDKVFDAGIAGMGGASFPTHVKLSPPSNVKIDVLII
                     NAAECEPYLTVDDALIKRSYKEIIEGIMITRTILGVKNVIIGVESNKKRSYKTIKEYL
                     KKIGEKNIKISMLKTKYPQGAEKQLIFSLTHRTVPSGGLPMEVGTVVQNIGTCFAIQQ
                     AVCYGTPLFERLVTVSGSLINKPGNYKIRIGTTIADIIRECGGLKEQPHKIVMGGPMC
                     GQAVESTDIPIVKGTSGLLFLSKEESSAGRYANCIRCGRCVQACPINLLPFEIAANIE
                     ISRPECTERFSPLDCIQCGACSFICPSRRPVSHFVKVAQDYIRSKR"
     gene            1950..3020
                     /locus_tag="P5123_03415"
     CDS             1950..3020
                     /locus_tag="P5123_03415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013780781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RnfABCDGE type electron transport complex
                     subunit D"
                     /protein_id="HRX15339.1"
                     /translation="MDQQIYTLSLAPHTKNRQNLPVVMWLVVLSLLPSTVFAVYTFGM
                     HVLLVILTAIFSACAAEYLFRLLLKRELTIFNGSAAITGLLLAMNLPPMVPLWIPAVG
                     SVFAIIIAKELFGGLGHNIFNPALVGRAFLMASWPALMTTKWITFPNGTLFSSSFNAY
                     SVLPKPALDALTGATPLAALKNVPSLAKSFDVHSQYNIYQTLFDTSNFKALAIGNIGG
                     CIGETSALLLLTGALLLLFTRIINWRIPFSYIATFALLIFTYYKLTGFFMPYHALVYH
                     LFSGGLILGAFFMATDMVTSPVTGKGMLIFGAGCGLLTFAIRIWGGYPEGVSYSILLM
                     NAAVPLIDKYCVPKIFGKRKQA"
     gene            3020..3673
                     /locus_tag="P5123_03420"
     CDS             3020..3673
                     /locus_tag="P5123_03420"
                     /inference="COORDINATES: protein motif:HMM:NF016121.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FMN-binding protein"
                     /protein_id="HRX15340.1"
                     /translation="MKDIIKPAAVLAAVTFCASLLLATVFAVTKERIAFLDSEAEKNA
                     LAIVLPGYSITDTAKTKDNKIYWVGEKTENGETINGFAFISESPGYSGTIRCMIGVDS
                     NMTILGISILKQTETPGLGARSVEIASKNTFWDVITGSAVDEGIQFPWFQQQFAGLNG
                     NKTVQVVKNGDWTPEMASDLVSQNAVSAITGATITSKAVVDCIHKGISEYNDIIKKK"
     gene            3689..4288
                     /locus_tag="P5123_03425"
     CDS             3689..4288
                     /locus_tag="P5123_03425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019245128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0022900 - electron transport chain
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="electron transport complex subunit E"
                     /protein_id="HRX15341.1"
                     /translation="MSIKKEFLKGLVEKNPVMVLGIGLCPALAVTTSVSNALWMMIAT
                     TFVLTCSNILVSLVKDYIPSHIRIPVFITIIATFVTVVEITLKAYQPEVYTSLGIFIP
                     LIVVNCIILGRAEEFASRNGVFISIIDALGMGIGFGLTLTILAFFRETLGANKLFGYT
                     LIPGFKPAGMMAIAPGAFITLGLMLWAMNRIMVSRKHKK"
     gene            4321..4920
                     /gene="rsxA"
                     /locus_tag="P5123_03430"
     CDS             4321..4920
                     /gene="rsxA"
                     /locus_tag="P5123_03430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006315707.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0022900 - electron transport chain
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="electron transport complex subunit RsxA"
                     /protein_id="HRX15342.1"
                     /translation="MEIKLFITILFSTIFINNYVLSQTLGICPFLGVSKKTDSAAGMG
                     FAVIFVMTLASVITYFIYKYILLPFSITYLRTIAFILVIASIVQLVEMAINKLSPSLY
                     RALGIFLPLITTNCAVLGVAVLNVDSGFITKEFGLLMTIVQGFGAGVGFLLALMLMSG
                     IRERLETADIPFNLRGMPISFIIAGLLSLAFLGFAGMSF"
     gene            4920..5552
                     /gene="thyX"
                     /locus_tag="P5123_03435"
     CDS             4920..5552
                     /gene="thyX"
                     /locus_tag="P5123_03435"
                     /EC_number="2.1.1.148"
                     /inference="COORDINATES: protein motif:HMM:TIGR02170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050797 - thymidylate synthase (FAD)
                     activity [Evidence IEA];
                     GO_process: GO:0009265 - 2'-deoxyribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent thymidylate synthase"
                     /protein_id="HRX15343.1"
                     /translation="MNKVQIQVQLLSITPDCEKLIEEAGRTCYLSFDKTGSNTSFISN
                     AIRRGHLSIIEHACASFRIKGASRAFTHQLVRHRVASFSQQSQRYVDENNFNYTIPPS
                     IETNAQALKLYNSFMEQARDVYTNLRSMNILKEDARFVLPNALESEIVFTANFRELRH
                     LFDLRLKKSAQWEIRMAALQMLQIVKNIAPQVFGDYIIDSENMTAVSENI"
     gene            5571..6266
                     /locus_tag="P5123_03440"
     CDS             5571..6266
                     /locus_tag="P5123_03440"
                     /inference="COORDINATES: protein motif:HMM:NF014762.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent transcriptional regulator"
                     /protein_id="HRX15344.1"
                     /translation="MEKSCNQNHSKNEKHYYDTSLSANMEDYIETIALLSKKNRVVRV
                     KDIARELNIKMPSVSAALQKLKELNLLDYEKYGFIELTDYGFKIAEKIYDKHSLIAGF
                     LHDLLKLDSNQSSEEACRLEHYLSAESCSQIYKLMQFYKNESAVSSEWTIKLNSIMNC
                     RTLINYTVGDKLKIIDQGEVNDSICPVGALCTIVEINNDCSVLSLSCNGKNMDIPFEA
                     ACTIYASYAETGL"
     gene            6263..6907
                     /locus_tag="P5123_03445"
     CDS             6263..6907
                     /locus_tag="P5123_03445"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRX15345.1"
                     /translation="MKRITIVSFVIILLVSVSFFFIRATTMMPDCSALESVSKSCSNF
                     KYNKLFTRALMTALEEKDKPACYYIMKLFYHRNRLNDESFNLHYRLSLNLINSAGYNT
                     EEIPILLTMLMIRESGKYFEDDDQLICLFLSSLRNIDLVGADAISMYYFGKRHYNLSA
                     TESIRLAVLCSLPNESAYSQSDQFRQTAREVVLILFRRNVISESEYKTIIHEFR"
     gene            complement(6900..8567)
                     /locus_tag="P5123_03450"
     CDS             complement(6900..8567)
                     /locus_tag="P5123_03450"
                     /inference="COORDINATES: protein motif:HMM:NF012503.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016831 - carboxy-lyase activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0019752 - carboxylic acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal-dependent decarboxylase"
                     /protein_id="HRX15346.1"
                     /translation="MIDKLDADIEHLRKLFVFPESQDRFLEFGKQILDMMYNSFKQKG
                     GLHSSITLDELSSLFSDISIPRDPKLLRDIFPEIQNKIAAHSVKVYSPYYIGHMTSAI
                     PYFTILIEMIVSSLNQNQVKIETAKASTYVERELLCWIHRLIYRKNPRFYKETIQNHR
                     VALGNVTSDGTMANLTAMLVARNLLFKEDGRFPGIASAGLAEAYNHYNCEKTVLLMSK
                     RGHYSFDKIADLCGIGDNNIIKIPVENGTNKIDINVLSKELNSIEDYNKNHEKKIKIL
                     SLIGIAGTTETGNIDNLYEMNKLAKKHKTRFHVDAAWGGPVLFVEDYAHLFKGIEEAD
                     SVTFDSHKLLYLPLSMGMVLFNSEKDLQSLEHTTQYVVRRDSRDQGRFTIEGSRPFAA
                     LRPWVALKVIGSEGFRLLFEKAFTISSYFKTQIDLHSNFESLNEPELFIFNYRFIPNR
                     IKKHLESLMAEKSEASLKQATRINNELNALNIQLHREIRNNDLSFVSRTVIESTKYSP
                     DEIVVLRAVTINPLTTNSIIKEIIAEHNRLGLRIFNKSWKKKFKNIT"
     gene            complement(8557..8997)
                     /locus_tag="P5123_03455"
     CDS             complement(8557..8997)
                     /locus_tag="P5123_03455"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRX15347.1"
                     /translation="MKTKDSTIMFAASAVIALAAIIILVASVYSRMFYEKRINNLMNG
                     SPNKLTEAYILLLDPSPFAGYTNIDGSSILTSTLSVLNTHYALNGQIDEKYIEDVKTL
                     LGYRMMGTTLGLKSSVYFFLISAALSIAGLISRKKENSLKQNDR"
     gene            complement(9063..9776)
                     /locus_tag="P5123_03460"
     CDS             complement(9063..9776)
                     /locus_tag="P5123_03460"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF016347.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RsmE family RNA methyltransferase"
                     /protein_id="HRX15348.1"
                     /translation="MPQYPVDDNFDFSVGKEVRLYGANFHHIINVRRHKNGDLIVVRP
                     KDGSFISGELVSVQKESCIICVTDIKPAVQNYLKLTLAMPILKGKSNDFVIEKCVESG
                     VTGIIPVFYNRCVPESSSINKKQERWQKIIEETFKQCMAPVMPLLMPSISSKELFVTK
                     DVTSLIADVHASDKLDAKPFTDEIIVSIGPEGGFDESEKTQAAQNGWKSFRLSVNQLK
                     AETACIVIPAVVRELSLFS"
     gene            complement(9776..10537)
                     /locus_tag="P5123_03465"
     CDS             complement(9776..10537)
                     /locus_tag="P5123_03465"
                     /inference="COORDINATES: protein motif:HMM:NF028441.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_process: GO:0042953 - lipoprotein transport [Evidence
                     IEA];
                     GO_process: GO:0044874 - lipoprotein localization to outer
                     membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane lipoprotein-sorting protein"
                     /protein_id="HRX15349.1"
                     /translation="MRKIQILLLIIIIPLTASAQDTRIVEITAQEILARVDDVMAYPS
                     GKLEGKLMHIFPDGRSVTRDILGYIDKDNSLFTFSSSSRGNEIRVLYNLQGEDIWVYN
                     LHSLKLFHKADVDRYDRVFDSNYSFIDISNADLQSNYTGSILRKTTYKGSEVVILSLT
                     PIFKNGLYGKLTLYVTASNYLPLRIDYHDNDMAILKTLSFTKMSTFEKRHFPVRYDML
                     HISSGTLSIIEFTDYDKKVTFPLSTFRHENMSSVK"
     gene            complement(10587..11207)
                     /locus_tag="P5123_03470"
     CDS             complement(10587..11207)
                     /locus_tag="P5123_03470"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRX15350.1"
                     /translation="MTFVETLQNLKWADYLYCLLFDNERLSVMLEEGKPFLPATIVFP
                     VIHALFVILSGSVLNNDGSLFFIKLSYGFISLSILWVLVNIFVTCLSSFTLSGMKIRP
                     DMMRIFSIINLSYFPRLFVLPITVFLVSIHPENSSPGLWSTLSSFVLTIYSIVITVKT
                     ICFLHKTTMMKSFLSVVMSLFFAICALLLVAVCASMIMFYMLSQNM"
     gene            complement(11204..12142)
                     /gene="sppA"
                     /locus_tag="P5123_03475"
     CDS             complement(11204..12142)
                     /gene="sppA"
                     /locus_tag="P5123_03475"
                     /inference="COORDINATES: protein motif:HMM:TIGR00706.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006465 - signal peptide processing
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptide peptidase SppA"
                     /protein_id="HRX15351.1"
                     /translation="MDKNRQLLTVIVLFLALTAILSIVRISISMNNSGAKSKLGFPDN
                     SPGIGVIQIYGPITTSGESGAFSQSGGSDAIVEKLDQMQNDPKIKAVVIRINSPGGTV
                     AATQEIFRKITEMRQQNIPVVASMSDLAASGGYYVASACSYIFANQGTITGSIGVIIS
                     SPNFKDLFEKYGISMNVIKSGRYKDILSSSRELEDDERELLQSVIDNTYRQFLKDVSL
                     GRNMPIDEFSDYADGRIFSGSQALEYRLVDEIGTYSDSIKKAKGLARLEDNAPVYLES
                     TSPFDQFFKSLEGRISSFDTPAALLNHSLVEYRYVQ"
     gene            12509..13729
                     /locus_tag="P5123_03480"
     CDS             12509..13729
                     /locus_tag="P5123_03480"
                     /inference="COORDINATES: protein motif:HMM:TIGR01499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Mur ligase family protein"
                     /protein_id="HRX15352.1"
                     /translation="MSFENHLNSFINNENSKGNFRNYNPESIKKAAAVIGNPQNEYKI
                     FHIAGTNGKGSSALYIAQILERSGYKTGLFTSPHLLAVNERIAINRQNISDIDAVKII
                     DKITAACDTSGLTWFDMITLVAYEYFREKQVQFAVIECGLGGRLDSTNIMQDSNPLIT
                     SISLDHTLLLGNTLDKITSEKAAIIKSSGIAVTSNTEKEIVDVIEDQSKKANASLKIY
                     GRDFIYNIREDNKLLVYYGSRSFNGINLSLTTPEQALNFSSALALLVASKTEFDESIL
                     YQDPFEQPFGRFTVLNTKPLIVFDCAHNISSIKAMLDTCKSRQMTKGTVFLSLMADKE
                     CETIVSMIKKSSFSLVYVLLNDQRSWIPDNGSCTIMEEDNALSYIDHDGINLFCGSFR
                     LFNKTADIINKTKN"
     gene            13752..14615
                     /locus_tag="P5123_03485"
     CDS             13752..14615
                     /locus_tag="P5123_03485"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRX15353.1"
                     /translation="MKNELNEIINECVRLSENLYLGTDKDEVDKEFQKLKNRHEFALS
                     KTEYKSEELKPVYTSFLNSFNADFFSLYSDLTITEMLKTKCYQAGDNSILNFVDLPNI
                     EELSTDTTHRIKLNLQKDNIYLYLYKNSETNICFRTFSSSPFSTIDNFNKTVDKLNNF
                     ILNLNNQSYDYTWFNSIINDKLINYKNDLFLFYYNFKKLYGIFSHLGIDTVEDIAEYI
                     INRINEKYPDAIMVYQLSLEKVCVFFQSEVQDKRIIFEYNEIPIPYVMKKVLVKGSSK
                     FEKLFEYIAED"
     gene            complement(14725..16533)
                     /gene="rpoD"
                     /locus_tag="P5123_03490"
     CDS             complement(14725..16533)
                     /gene="rpoD"
                     /locus_tag="P5123_03490"
                     /inference="COORDINATES: protein motif:HMM:NF004208.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor RpoD"
                     /protein_id="HRX15354.1"
                     /translation="MSKSDVALDSNAEIQKLINLGKTNGEITYDEINDILPDKLLNSE
                     KIDDVFILINQLGIEVVEESTRKGAVLADVHGAAAIIAEKKPATKKKAAKSAKKAAKK
                     KDDSAIDDPIRLYLREIGKVSLLSGEEEVDLAKKIEEGEILIEEAVIRSTLLINDLIK
                     NHPKVKQGKIKLTDILKINRLYYFSSFDLKALEERYYTNMDIIIKEDKLMIQAMTRLK
                     KTDIDEETIKKAEEQYKKSYEVIVDHIKAIDIHENEIGKLSSKIQSMVSRIKDTKKFF
                     SDIEKKYKTDIKTIKHDARKLEKDEDTAEILTKYKVNDSEIINEIVKDIRNNERKIRR
                     IEQEAGTNAEDILEWGKQINMGQRKISVAKKQLINANLRLVVSIAKKYANRGMHFFDL
                     IQEGNIGLIKAVDKFEYRKGYKFSTYATWWIRQAITRAISDQARTIRVPVHMIEQINK
                     ITRETRLFQQEFGREPVPEELADRLGWPVAKVKQVKNVAREPISLETPVGEEEDSLLG
                     DFIEDKDVDSPANAAAYQLLTEHINKVLSTLPEREKRVIRMRFGLEDGYQHTLEEVGY
                     VFNVTRERIRQIEAKALRRLRHPTRSRKLKDYLEYI"
     gene            complement(16793..>18141)
                     /locus_tag="P5123_03495"
     CDS             complement(16793..>18141)
                     /locus_tag="P5123_03495"
                     /inference="COORDINATES: protein motif:HMM:NF019880.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="toprim domain-containing protein"
                     /protein_id="HRX15355.1"
                     /translation="IDIFELGFAPDSWDYIKNSLDKKSIALTEGLECGLLGHKKETGR
                     YYDMFRNRLMFPIRDINGKCLGFGGRVLDDSQPKYLNSPESAVFSKRSVLYGLNLAAE
                     EIRSLNRVIVVEGYLDVIGAYQSGIKNVVAPLGTALTEAQLTVLSHMCSEIVLLFDSD
                     AAGAKAALRTVSASHDFNASIRIGSLPEGDPFEYILKNGPRKLLMHVDTACSPAEFRL
                     RSHFKANSHKETKLLLREMFEILNDYQYEAEKEEGITIIAALLNIRKDSVQLDYQKYK
                     GVKPAATVTATPLKQKNFVQRSVEELIVLMLNYPSLIEKAMMDFHEFDIDDPLLKNLI
                     NTIFDLYEHDENVTGDKIFDIIEDNNEKRFLSERLSADYNIESPESAYTEIYLNMKIH
                     AIDVKLTKFNEALKKCKDSSDSSRILTEIDIWRREKEKLSGYIYSKNASMLSVQNH"
BASE COUNT         5607 a         3507 c         3234 g         5793 t
ORIGIN      
        1 aggatttttt aaaaaaagat tttgaatatc gattgacaga aaacgggggc gtaaatatat
       61 agtctactat aaccgcgggt gtagttcaat ggtagaactt cagccttcca agctgatagc
      121 gtgggttcga ttcccatcac ccgctcatat ttttcttcac ttttaaaact ctccaggcac
      181 cctgaaaaaa tgttcatcct atgaaatatt aaaactatag gaacaaaaat agtaaaaata
      241 gttaaaatat aattgtctgt tctgcttcaa aaaaaatact ttacaatagc atacatatct
      301 cttcaattga ctgataagtt tcgtatcggg agacaactat gactaaagag aagattttct
      361 gcgctaactg cagaaattgt attgtaataa gacaacatga agcagaaccg gataagtatg
      421 tactacgtgt aaaatgtaag cttcgtaaat gggttaaacg ttctggagaa gaaaagctct
      481 ataaatactt taccgttgct cgccgtatta tggatgactg tgacaactac gaagaatctg
      541 gagagctttt tccatacatt aaaaacctcc gcaaagagct tccaatcaag gatgaaattt
      601 actctaccaa ggatcagtag tcaatgttca atccttcttt caagggtggt gttcaccccc
      661 ctgaaaacaa aaaactcaca gaagagaggt ctattgaaaa cctctctgtt cctcatacct
      721 gttttgttcc tgtacaacag catataggta aaccagccat acctgttgta caggtcggtg
      781 attatgttca ggtcggtcaa cttatagcca aagctgacgg ttttatcagc tctaatattc
      841 acgcttccat acctggaaag attgcctcta tcacaaatgg tccaacaccg gcctctccat
      901 ccggtcttcg catacaaata gagactcacg gcaattttac cagcgaaaaa acacccgata
      961 aaattgaatg ggataaactt cctccgaagg atcttctaga caaggtcttt gatgctggta
     1021 ttgccggaat ggggggggca tcattcccta ctcatgtaaa actttcgcca ccttcaaatg
     1081 tcaaaataga tgtattaata attaatgctg ctgaatgtga gccctattta actgttgatg
     1141 atgcccttat taaacgttca tacaaagaga ttatcgaagg tatcatgata accagaacaa
     1201 ttctgggagt caaaaatgtt attatcgggg tcgaatcaaa caaaaaaaga tcatacaaaa
     1261 caataaaaga gtatcttaaa aaaattggcg aaaaaaacat aaaaatctcc atgctgaaaa
     1321 cgaaataccc gcaaggagca gaaaagcagc ttatattttc acttacgcat cgtactgttc
     1381 catccggtgg gcttccaatg gaagttggaa ctgtagtaca aaatattggc acctgtttcg
     1441 caatacaaca agccgtctgt tacggaaccc ctctttttga acggcttgtt accgtaagcg
     1501 gttctctaat aaacaaaccg ggtaactata aaattagaat tggtacaaca attgccgata
     1561 taattcgtga atgtggcggt ctaaaagaac agccgcacaa gatagtcatg ggtgggccaa
     1621 tgtgtggtca agccgttgaa agcacggaca tcccgattgt aaaaggtaca tccggcctcc
     1681 tctttctttc aaaagaagag agctcagcag ggcgctatgc aaactgtata cgatgtgggc
     1741 gctgtgttca agcctgtccc attaatcttt taccattcga aatagcagca aatatcgaga
     1801 tcagccgtcc tgaatgcacc gaaaggtttt ctccactaga ctgtatccaa tgcggagcat
     1861 gttcctttat ctgtccgtca cgtcgtccgg tcagtcactt tgttaaggtc gcgcaagact
     1921 atattagatc aaaaaggtaa ctttttatta tggatcagca gatatacacc ttatcactcg
     1981 cccctcatac aaaaaacagg caaaatcttc ctgttgttat gtggcttgtt gtcttgtcgc
     2041 tcttgccttc aacagttttc gccgtctata cctttggcat gcatgttctt ctggttatat
     2101 taaccgccat tttttctgct tgtgcagccg agtacctttt tcgtctttta ttaaaacgtg
     2161 aattaactat atttaacgga tcagcagcca ttactgggct tttacttgca atgaacctgc
     2221 ccccaatggt tcctctctgg ataccagcag tcggttccgt ttttgcgata attattgcta
     2281 aagaactttt cggtggcctt ggtcacaata tctttaaccc tgctcttgtc gggcgtgcat
     2341 ttcttatggc ttcatggccc gccttgatga ctacaaaatg gattactttc ccgaacggta
     2401 cattgttcag cagctcgttt aacgcctatt cagttttgcc gaagcccgcg cttgatgcgc
     2461 ttaccggagc gactccgctt gctgctctta aaaacgtacc gtctttagcc aagtcgtttg
     2521 atgtacactc gcaatataat atttatcaaa ccctatttga tacatcaaat tttaaagctt
     2581 tggcaatcgg taatataggt ggatgtattg gtgaaacatc ggctcttctt ttgctaacag
     2641 gcgccttgtt gctgctcttt acacgaataa ttaactggcg cattccattt tcatatatcg
     2701 caacatttgc tctattgata tttacttatt acaaattaac aggttttttt atgccgtatc
     2761 acgcgcttgt ttaccatctc ttttctggtg gtcttatact cggcgctttt ttcatggcaa
     2821 ccgatatggt aacttcgccg gtcaccggta aggggatgct tattttcgga gcaggatgcg
     2881 gattattgac atttgctata cgcatctggg gaggttaccc tgaaggagta tcctactcaa
     2941 tactattaat gaatgctgct gttccattaa tcgataaata ttgtgttcca aaaatatttg
     3001 ggaagaggaa gcaggcctga tgaaagatat tattaaacct gcggctgtcc ttgcggcggt
     3061 tacattttgc gcttcacttc ttttggcaac agtatttgct gttacaaaag aacgtatcgc
     3121 ttttcttgac agtgaggccg aaaaaaatgc gctggctata gttttaccag gctactcaat
     3181 caccgatacg gccaaaacaa aagataataa aatatactgg gtaggcgaaa agaccgaaaa
     3241 cggtgaaacc attaatggtt tcgcctttat aagtgaatct ccaggttatt ctggcacaat
     3301 acgatgcatg ataggtgttg actctaacat gactatttta ggcatatcga ttcttaaaca
     3361 gaccgaaact cccggtcttg gagctcgttc agtagagata gcatcaaaaa ataccttttg
     3421 ggatgtaatt accggttcag cggttgatga aggaattcag ttcccctggt ttcagcaaca
     3481 gttcgccggt ttaaatggta ataaaactgt tcaggttgtc aaaaacggtg actggacacc
     3541 ggaaatggct tcagatcttg tttcgcaaaa tgctgtttca gcaataacag gagcaactat
     3601 tacatcaaag gctgtagttg actgtattca caaaggtatt agcgaatata acgatataat
     3661 taaaaaaaag taataaatag agattgttat gagtattaaa aaagagttct tgaaaggtct
     3721 tgtcgagaaa aatcctgtta tggttttggg tatcggtctt tgccctgcct tggcggtaac
     3781 tacttcggtt tctaacgcct tatggatgat gatagcaaca acttttgtct tgacctgctc
     3841 aaatattctg gtatctcttg ttaaagatta tataccgtca catatacgaa tacccgtttt
     3901 tattacaatt atagctacct ttgttactgt tgtcgaaata acattaaaag cttatcagcc
     3961 agaagtatat acaagtcttg gtatctttat tcctcttatc gttgttaact gtatcatcct
     4021 tgggcgtgca gaagagtttg cttcccgtaa cggtgttttt atatcaatta ttgatgcctt
     4081 gggcatggga ataggttttg gtttaacgct taccattctt gctttttttc gggaaacact
     4141 tggggcaaat aaactttttg gatacacact gatcccgggt tttaaacctg caggaatgat
     4201 ggctattgcc cctggagcct ttatcacctt gggtctaatg ctctgggcaa tgaatcgaat
     4261 tatggtttcg cgcaaacata aaaaatagtc ataaactttt gattaccggt aaacaataat
     4321 atggaaatta aactttttat aacaattctc ttctccacaa tatttattaa taactatgtt
     4381 ctttcgcaga cccttggtat ttgtcctttt cttggtgtat caaagaaaac cgattctgcg
     4441 gcaggaatgg gtttcgccgt aatctttgtc atgacattgg cttccgtaat tacatatttt
     4501 atttacaagt acatacttct gcctttcagt atcacctatt tgcgtaccat tgcctttatt
     4561 cttgttatcg cgtcaattgt tcaacttgtc gaaatggcta ttaacaagct ctcaccctct
     4621 ttataccgag cacttggaat ctttttacct ctgataacaa caaactgtgc ggttttaggt
     4681 gttgctgttt taaatgtcga ttccggattc ataacaaaag agttcggcct attaatgaca
     4741 attgttcaag gattcggtgc cggtgtaggt ttcttgctgg cacttatgct tatgtctggc
     4801 attcgagaac gtcttgaaac tgccgatata ccattcaatt tacgtggtat gcctatatct
     4861 tttataatag caggtctgct ttcactggct tttcttggat ttgcagggat gagtttttaa
     4921 tgaataaggt tcagatacag gtacaacttc tgtcaattac ccctgattgc gaaaaattga
     4981 tcgaagaggc aggccgaact tgctatctct cttttgacaa aacaggctct aataccagct
     5041 ttattagtaa cgcaattcgc cgtggacatt tatctattat tgaacatgcc tgcgcttctt
     5101 ttcgtattaa gggagcatca agagccttta cacatcaact tgtgcgtcat cgcgttgcta
     5161 gtttttcaca acagtcacag cgctatgtag acgaaaataa ttttaattat acaattcccc
     5221 cgtctatcga gaccaatgct caggcattaa aactttataa ttcatttatg gaacaagctc
     5281 gtgatgtcta taccaacctg cgctcaatga atattctaaa agaggatgca aggtttgttt
     5341 taccaaatgc tcttgaaagc gaaattgttt ttacagctaa tttcagagag ctaagacatc
     5401 tgtttgattt acggctaaaa aaatctgcgc aatgggaaat acgtatggcc gctcttcaga
     5461 tgcttcaaat agtgaaaaac attgctccac aagtttttgg tgattatatt atagactctg
     5521 agaacatgac agcggtttca gaaaacatct aaacgggagc agcattattg atggagaagt
     5581 cgtgtaacca aaatcactca aaaaatgaga aacactatta cgatacatca ctttcggcga
     5641 acatggaaga ttatatcgaa acgatagctc tgttgtctaa aaaaaacagg gttgttcgag
     5701 ttaaggatat tgctcgtgaa ctgaatataa agatgcccag tgtatcggct gctcttcaaa
     5761 aacttaaaga gttaaacctt ctagactacg aaaaatacgg atttatcgag cttactgact
     5821 acggatttaa aattgccgaa aaaatatatg ataagcactc gcttatcgca ggctttttgc
     5881 atgatctgct aaagctagac agcaatcagt catcagaaga agcatgtcgg cttgaacact
     5941 acttgtctgc ggagagctgc agccagattt ataaactaat gcagttttat aaaaacgaat
     6001 ctgcggtaag ttcggaatgg acaattaaac ttaacagcat tatgaactgt cgaacattaa
     6061 taaactatac tgttggggac aaactgaaaa tcatagatca gggagaagtg aatgactcca
     6121 tttgtccggt tggtgctcta tgcacaattg tcgaaataaa taatgactgc tctgttctaa
     6181 gcctctcttg taacggaaag aatatggata tccccttcga agctgcttgt acaatatatg
     6241 caagttatgc cgagacaggc ttatgaaacg aattactatt gtttcatttg taataattct
     6301 actggtttcc gtttcatttt tttttattag agccaccaca atgatgcctg actgctccgc
     6361 tcttgaatct gtttcaaaat cttgttcaaa ttttaaatat aacaagttat ttacaagagc
     6421 ccttatgact gctctggaag aaaaagataa accagcctgt tattatataa tgaaactctt
     6481 ttaccacaga aacagactca atgatgaaag ctttaacctt cactaccgtt tatcactaaa
     6541 tttaataaac agtgctggat acaataccga agaaattcct atactattga ccatgctaat
     6601 gatacgtgaa tctggaaaat attttgaaga cgatgaccag ctaatatgtc ttttcctttc
     6661 ttcattaaga aatatcgatc ttgttggcgc cgacgcaata tcaatgtatt attttggtaa
     6721 acgtcattat aacctttcgg caactgaatc aatcagactt gctgtgttgt gctctttacc
     6781 taatgaatcg gcctattctc aatcagatca gtttcgacaa acagcaagag aagttgtgtt
     6841 aattcttttc aggcgaaacg taatatccga atccgaatat aaaaccatta ttcatgaatt
     6901 taggtaatat ttttaaactt ctttttccat gatttattaa atattctcaa gccaagacgg
     6961 ttatgttcag caattatctc tttaattatg ctgttggttg taagcgggtt tattgtcaca
     7021 gcccttaata caactatctc gtctgggcta tattttgtcg attcgataac tgtccgagat
     7081 acaaacgaaa ggtcattgtt acgtatttct cgatgaagtt gtatatttaa tgcattcagt
     7141 tcattgttaa ttcttgttgc ttgtttaagt gaagcttcac ttttttcggc catcaatgat
     7201 tctagatgct tttttattct gtttggaata aagcggtaat taaagataaa tagttcgggt
     7261 tcgtttaaac tttcaaaatt ggaatgaaga tcaatctggg ttttaaaata agagctaatt
     7321 gtgaaagctt tttcgaatag aagcctgaaa ccttcagaac cgattacctt taaagcgacc
     7381 catggtctaa gagctgcaaa tggtcgtgat ccctcaatgg taaaacgccc ctgatcccgt
     7441 gagtcacgac gaacaacata ttgtgttgta tgctccagtg actgtaaatc cttttcactg
     7501 ttaaacagaa ccatacccat agataaagga agataaagca gtttatgcga atcgaaggta
     7561 acagaatcgg cttcttcgat gcctttaaaa agatgagcat aatcctcaac aaaaagaacc
     7621 ggacctcccc aggccgcatc cacatgaaaa cgggtcttat gctttttagc caacttattc
     7681 atctcgtaca aattgtcaat attgccggtt tcggttgtac ctgctattcc tattagagat
     7741 aatatcttga tctttttctc gtgatttttg ttataatctt caatcgagtt tagctctttt
     7801 gaaagaacat ttatatcgat tttattcgta ccattttcaa caggtatttt gataatatta
     7861 ttatcaccta tgccgcaaag gtcggctatt ttgtcaaacg agtagtgccc tctctttgac
     7921 ataagtaaca ctgttttttc gcaattatag tgattatagg cctcagcaag cccagcagat
     7981 gctattccag ggaaacgtcc atcttcttta aacagaaggt tgcgtgccac aagcatagca
     8041 gttaaattag ccattgttcc gtcactggtg acattgccaa gagcgaccct atgattctgt
     8101 attgtctctt tataaaagcg cgggttttta cggtaaatca gtctgtgtat ccagcaaaga
     8161 agctctctct ctacataagt tgatgcttta gcagtctcga tctttacctg attttgatta
     8221 agtgagctaa ctatcatttc tataagaata gtaaaatatg gaattgcact tgtcatgtgc
     8281 ccaatataat agggactgta aaccttaaca gaatgggcag cgatcttgtt ttgaatttca
     8341 gggaaaatat cacgtaaaag ttttgggtcg cgaggtatcg atatatcgga gaaaagagac
     8401 gaaagctcgt caagagtgat agagctatgc agcccgccct tttgtttaaa ggagttatac
     8461 atcatatcca gaatctgctt accaaactca agaaaacggt cctgcgattc aggaaaaaca
     8521 aagagcttgc gaagatgttc tatgtctgca tcaagtttat cgatcatttt gtttcaaaga
     8581 attctctttt tttcggctaa taagtccggc tatggaaaga gcagcactta tcaaaaaaaa
     8641 gtaaaccgag ctttttagac caagagttgt acccatcatg cggtaaccta aaagcgtctt
     8701 aacatcttca atgtactttt catcaatttg accattaagg gcgtaatgcg tattaagaac
     8761 agacaatgtt gaagttaaaa tcgaagatcc atcaatattt gtgtatcctg caaatggtga
     8821 tggatccagt aacaagatat aggcttctgt caatttattg ggcgaaccgt tcatcagatt
     8881 attgattcgt ttttcataaa acattctaga ataaacagat gcgacaagaa taattattgc
     8941 tgccaaagca ataacggctg aagctgcaaa cattattgta ctgtcttttg ttttcataaa
     9001 tactccagag agataaaaac agtgttaaac ctgcgtcttg tttacggcat ctttctcaat
     9061 cgtcaagaaa ataaactgag ctcgcgtact actgctggta ttacaataca ggcggtttct
     9121 gcttttaatt gatttaccga cagcctaaag gacttccatc cattttgagc agcctgtgtt
     9181 ttttcgcttt catcaaaacc accttctggt ccaatagaga caataatctc atcggtaaac
     9241 ggttttgcat caagtttgtc actagcgtgc acatctgcta tcagagatgt aacatctttc
     9301 gtaacaaaaa gctctttcga gctgatagac ggcataagaa gcggcataac cggagccata
     9361 cattgtttaa aagtttcttc gattattttt tgccaacgtt cctgtttttt atttatagaa
     9421 ctactttcag gaacgcatcg gttgtaaaaa acagggatta ttcctgttac gccactctca
     9481 acacattttt caattacaaa gtcgttgctc ttacctttta agataggcat agcgagagtc
     9541 agttttagat aattttgcac ggcgggttta atatcggtaa cgcaaattat acagctctct
     9601 ttttgtacgg aaacaagttc accgcttata aatgaaccgt cttttggtcg tacaacaatc
     9661 agatccccat ttttgtgacg tcgaacatta ataatatggt gaaagttggc tccgtatagc
     9721 ctaacctctt ttccaacact gaaatcaaaa ttatcatcga ccgggtactg gggcattatt
     9781 taacagaact catgttttcg tgtctaaagg tagacaaggg aaaggttact tttttatcat
     9841 aatctgtaaa ttcaattatg gacagtgttc cgctggatat atgaagcata tcatatctta
     9901 caggaaaatg tcttttctca aaagttgaca tttttgtaaa cgagagtgtt ttaagtatgg
     9961 ccatatcgtt atcatgataa tctattcgaa gaggaagata attggaagct gtcacataaa
    10021 gggttagttt accataaagg ccattcttga atattggggt caatgaaaga attactacct
    10081 cactcccctt gtatgtagtt tttcttaaaa tggaaccagt atagttactc tggagatcgg
    10141 cattagaaat atctataaaa gagtagttag aatcaaaaac tctatcgtat ctgtccacat
    10201 ctgctttatg aaaaagttta agagaatgca ggttatatac ccagatatcc tcaccctgca
    10261 gattataaag aacacgaatc tcattaccgc gtgaactact cgaaaaagta aatagtgaat
    10321 tgtctttgtc aatgtatcca agaatatccc ttgttacaga acgaccgtct ggaaagatat
    10381 gcatcagctt accttcaagt ttgccagagg ggtaagccat aacatcatca actcgcgcaa
    10441 gaatttcctg ggcagtaatc tcaactattc ttgtatcctg tgcagaagct gtgagaggaa
    10501 taatgataat taaaagcaat atttgaattt ttcgcattaa ataaatttcc gttaatttaa
    10561 aatatataat tgtatagaat cagtaatcac atgttttgtg acagcatata gaacataatc
    10621 atagaagcac aaacggcaac taaaagtagt gcacagatag caaaaaaaag cgacataaca
    10681 acggataaaa aacttttcat catggttgtt ttatgcagaa aacagatggt ttttacggta
    10741 attacaattg aataaattgt cagaacgaaa gatgagagag tcgaccataa gcccggtgaa
    10801 ctgttctcag ggtgtataga taccaaaaaa accgttattg gcaaaacaaa tagtcttgga
    10861 aaataagata aatttattat ggaaaagatt ctcatcatat ccggtcttat tttcattccg
    10921 ctaagggtaa aagaagacaa acaggtgaca aaaatattaa caagcaccca aagtatggaa
    10981 agtgatataa aaccgtaaga taattttata aaaaacagag agccgtcgtt gttaagaaca
    11041 gaacccgaca atattacaaa cagggcgtga ataaccggaa acacaatggt cgccggtaaa
    11101 aaaggtttac cctcttccaa cattactgat aatctttcgt tatcaaaaag cagacaataa
    11161 agatagtctg cccatttcag gttttgcaaa gtttcaacaa atgtcattgt acgtacctgt
    11221 attctactag tgagtggttt aataatgccg ctggtgtgtc aaaagatgaa attctacctt
    11281 ccaatgattt aaaaaattga tcaaaggggg aagtcgattc cagataaaca ggagcgttat
    11341 cttcgagtct ggccagtccc tttgcctttt taattgagtc agaataggta cctatctcgt
    11401 ccacaagacg gtactcaaga gcctgagatc cgctaaagat acggccatca gcataatcac
    11461 taaattcatc aattggcata ttacgcccaa gagaaacatc ttttaaaaat tgtctgtatg
    11521 tattgtctat aacagactgt aatagttcac gttcgtcatc ttcaagctct cgtgatgaag
    11581 aaagaatatc tttgtatctt ccacttttga ttacattcat actgataccg tatttttcaa
    11641 aaagatcctt aaaattgggt gacgaaatta ttacaccaat tgatccagtt atggtaccct
    11701 gattcgcaaa aatataactg cacgcactag ctacataata gccaccagaa gcagctaaat
    11761 cactcataga agctacgacc ggaatgtttt gttgacgcat ctccgttatt tttctgaata
    11821 tttcctgggt agcagctact gtgccacctg gagaattaat gcgtattaca acagccttaa
    11881 tttttggatc attttgcatc tgatcaagtt tttcaacaat cgcatcagaa ccaccagatt
    11941 gcgaaaaagc tccgctttca ccagacgtgg taatcggtcc gtaaatctgt ataacaccaa
    12001 ttccgggcga attatcagga aagcccagtt tgctttttgc acccgaatta ttcattgaaa
    12061 tactgattct aacaattgac aagatagctg ttaaagccaa aaataatact attaccgtta
    12121 aaagttgtct gtttttatcc atattgtgtt attgacctga aaatagatag gattaccgta
    12181 tactttttaa atagaaactt aaatagaaac ggcagctcta tatacctctc ttaccttaca
    12241 tttattttca attacttttt ttaacaaccc gttaaaattt gatgcaaccg gtctacttta
    12301 cattgattaa atttatatca gtgaatttcg atttattacc attcactgaa cagcgatgcc
    12361 gcttaaaggc gtgtcaaata gagttctatt aaatattatt gtattgacca attacattat
    12421 tcaataactc tgtttaatct ggttaacagt aaactgtatc agttatacgt gtatttatca
    12481 tatttttaac gaggtagcga cggcaattat gtcattcgaa aaccatctaa acagttttat
    12541 aaataatgaa aacagcaagg ggaattttag aaattacaat cccgaatcaa taaaaaaagc
    12601 tgcggctgtt attggaaacc ctcagaacga atataaaatt ttccatattg ccggaacaaa
    12661 tggaaagggc tcatcggcac tttatatcgc ccaaatttta gaacgctccg gttataaaac
    12721 tggtctattt acttctcctc atcttcttgc tgtaaacgaa agaattgcca taaacaggca
    12781 aaatatatct gatattgatg cggtaaaaat aattgataaa ataacagctg cttgcgatac
    12841 atctggttta acctggtttg acatgattac tttagttgcg tatgagtact tcagggagaa
    12901 gcaagttcaa tttgcggtaa tagaatgtgg tcttggcgga agacttgatt ctactaatat
    12961 tatgcaagat tcgaatccac ttatcacatc aatttctctt gaccacacat tgcttcttgg
    13021 taatacatta gacaaaataa cgtccgaaaa agccgcgata ataaagagtt caggtattgc
    13081 tgtaacatca aataccgaaa aggaaattgt tgatgtaatt gaagatcaaa gcaaaaaagc
    13141 caacgcctct ctaaaaattt acggtcgtga ctttatttat aatattagag aagacaataa
    13201 acttttagta tattacggct caagatcttt taacggaatt aacttgtctc tcacaactcc
    13261 ggagcaggct ctcaattttt catcagccct agccctgctt gttgcatcta agactgaatt
    13321 tgatgaaagc attctatacc aggatccttt tgaacaacct ttcggcagat ttaccgtgct
    13381 taatacgaaa ccgttgattg tttttgattg cgcgcataac atcagttcca taaaagccat
    13441 gttagacacc tgtaaatccc gacaaatgac aaagggaaca gtatttcttt cgcttatggc
    13501 agataaagag tgtgaaacaa tagtaagtat gataaaaaaa agttcttttt ctcttgttta
    13561 tgttctttta aatgatcaac gttcatggat tccagataac gggtcttgca caattatgga
    13621 agaggataac gccttgtctt acattgatca tgatggaatt aatctttttt gcggttcatt
    13681 cagattattt aacaaaacgg ccgatattat aaataaaaca aaaaactgat ttcaaaattc
    13741 cggtaaaata aatgaaaaat gagttaaatg aaataataaa tgaatgtgtg cgtttaagtg
    13801 aaaatcttta ccttggaaca gacaaggatg aggtagataa agaatttcaa aaattgaaaa
    13861 acagacacga atttgcgctt tccaaaacag aatacaaaag tgaagagctc aaaccagttt
    13921 atacatcatt tctaaattcg tttaatgccg attttttttc tctctattca gatttaacca
    13981 ttaccgaaat gctaaaaact aaatgctatc aggccggaga caattcgatc ttaaattttg
    14041 tagatcttcc aaatattgaa gaattatcaa cagacacaac tcatcgtatt aagcttaacc
    14101 tgcaaaagga caatatatat ttatacctat ataaaaattc tgagacaaat atatgtttca
    14161 gaacttttag ctcgtcacct ttttcaacta ttgataactt caataaaact gttgataaac
    14221 taaacaattt tattcttaat ctaaataatc aaagttatga ctatacctgg tttaatagta
    14281 taattaatga caaacttata aattataaaa atgatctttt cctattctac tataatttta
    14341 aaaaattgta cggaatattt agccatcttg gaatagatac tgtggaagat atcgcagaat
    14401 atattataaa tcgcatcaat gaaaagtatc cagatgccat tatggtttat cagttgtcac
    14461 tggagaaagt atgtgtgttt tttcaaagcg aagtacagga caagcgaata atatttgaat
    14521 acaatgaaat accaattccc tatgtcatga aaaaagtact tgttaagggc tcgtccaagt
    14581 ttgaaaagct gtttgagtat attgcagagg attaggttat aacgttatta ttattattat
    14641 tattattatt tgtgacacaa aaaaaccctt taaggaaagg gtttttttat ggaaaaaagt
    14701 attagtctaa aacgacaata attatcaaat atattctagg tagtccttaa gtttccttga
    14761 tctggttggg tggcgcaaac gtcttagagc cttggcttcg atctgtctta tgcgttcacg
    14821 tgttacgtta aacacgtatc ctacctcttc gagtgtgtgc tgatacccgt cttccagacc
    14881 gaatctcatt ctaattaccc gtttttcgcg ttcaggcagc gtgcttaaca ccttgtttat
    14941 atgttcagtc agtagctgat acgctgccgc attagcaggt gaatcaacat ccttatcttc
    15001 aataaaatct ccaagaagcg aatcttcttc ctctccaaca ggagtttcaa gagatatagg
    15061 ctcacgcgcc acatttttaa cctgtttaac ttttgctaca ggccatccaa gacgatctgc
    15121 caactcttca ggaaccggtt ctcttccaaa ttcttgttga aataaacgcg tttcacgagt
    15181 tattttatta atctgttcga tcatatgtac aggaacacga atagttcgcg cctggtcaga
    15241 tattgcgcga gtaattgctt gacgaatcca ccaggtcgca tatgtcgaga acttataacc
    15301 tttgcgatat tcaaacttat ccaccgcttt gataagaccg atattgcctt cctgtataag
    15361 atcaaaaaaa tgcattccac ggttagcgta cttttttgca attgatacaa ctaatctcaa
    15421 gtttgcgttt atcagctgtt ttttcgcaac agatatcttg cgctgtccca tgtttatctg
    15481 tttgccccat tcaagaatat cttctgcgtt tgtaccagct tcctgttcta tacggcgtat
    15541 ttttctctcg ttatttctta tatctttaac aatttcatta atgatctccg aatcattaac
    15601 tttgtacttc gtaagaatct cagccgtatc ctcatctttt tcgagtttac gcgcatcatg
    15661 tttaattgtt tttatgtcgg tcttgtattt cttttcaata tcagaaaaga atttcttagt
    15721 atctttgatt cttgacacca tggactgaat tttcgaagaa agttttccaa tctcgttttc
    15781 atggatatca attgccttta tatgatcaac aatgacctcg taagattttt tgtactgttc
    15841 ttcagccttt tttattgtct cttcgtcaat atcagtcttt ttgagacgag tcatggcctg
    15901 aatcatcaat ttatcttctt tgataattat atccatattg gtataataac gctcttcgag
    15961 tgctttcagg tcaaacgagg agaaatagta taaacgattt atctttagta tatcggtcag
    16021 ttttatcttt ccctgtttta ctttgggatg attcttgata aggtcattta ttagtagagt
    16081 agagcgaata accgcttctt caataagaat ctcaccctct tctatctttt tggccaaatc
    16141 gacctcttcc tcgccggaaa gcagtgatac ctttcctatt tcacgaagat aaagacgtat
    16201 aggatcatcg attgccgaat catccttctt tttggctgct ttttttgcgc tttttgctgc
    16261 tttctttttt gtggcaggct tcttttctgc aataattgcg gctgcaccat gaacatccgc
    16321 cagaaccgcg cccttacgtg tagattcttc aactacttca attcccaatt gattaattaa
    16381 aataaataca tcatctattt tttcagaatt gagcagcttg tcgggaagaa tgtcgttaat
    16441 ctcgtcgtaa gttatctcac catttgtttt gccaagattt ataagcttct ggatctcggc
    16501 attagaatcc aaagcaacat cacttttgct catattacct ctttttaacg taattcacgg
    16561 gcctttaatt cttttttaaa agacgctctc ttttgctatc ctgttgtttt aaaggatctt
    16621 aaccagcatc ataataaaat acaatatgta aaagcccttc tgataatcgg ctttaagcct
    16681 gaaataaaaa tctacaaata tactaatatc acgtttggct ataattaact gctgttttta
    16741 aataaattca aattgctcca ttttagtaaa cagtagaata gaaatgttac cttcaatgat
    16801 tttgaaccga taacatggaa gcatttttac tataaatata tcccgatagc ttctcttttt
    16861 cccttcgcca aatgtctatc tcagtcaaaa tacgcgaact atcactgcta tctttacatt
    16921 tttttagtgc ttcattgaac tttgttagtt taacatcaat agcgtgtatc ttcatgttaa
    16981 gatagatttc tgtataggcc gattcaggac tttcaatatt ataatcggca gagagacgtt
    17041 cagaaagaaa ccgcttttcg ttattatctt caattatgtc aaatatttta tcacctgtta
    17101 cattttcgtc atgctcgtac agatcgaaaa tggtgttgat caaatttttt aaaagtgggt
    17161 cgtcaatatc aaactcatga aagtccatca tggccttttc aataagcgaa ggatagttaa
    17221 gcataagtac aatcagctct tctaccgatc tttgtacaaa atttttctgt tttaaaggtg
    17281 ttgctgttac tgtagcagct ggttttacac ctttatactt ctgataatcg agctgaacac
    17341 tgtcttttct gatattaaga agagcagcta taatagtaat accctcttct ttttcagcct
    17401 cgtattgata atcattcaaa atctcaaaca tttcacgcaa taacagcttt gtttccttgt
    17461 gactatttgc cttaaagtgg ctacgaagac gaaattccgc cggcgaacat gctgtatcga
    17521 catgcataag cagctttcgc ggtccatttt ttagtatata ttcaaacgga tcaccttcgg
    17581 gtagtgagcc gatacgtatt gaagcattaa agtcatgcga ggccgacact gttcttaaag
    17641 cagcttttgc gccagccgcg tcagagtcaa aaagaagcac aatttcgcta cacatatggg
    17701 ataaaaccgt aagctgggct tcagtaaggg ctgtaccaag tggcgcaaca acattcttta
    17761 tacccgattg atacgcccct attacatcaa gataaccttc cacaacaata acacgattaa
    17821 gggagcggat ctcttcggct gcaagattaa gcccgtacag cacggacctt tttgaaaaaa
    17881 cagcactctc cggtgaattc aggtactttg gttgcgaatc atcaagaact cttccgccaa
    17941 acccaagaca tttgccatta atatcccgta tgggaaacat taatcgattg cgaaacatgt
    18001 cataatagcg tccagtctct tttttgtggc caagaagacc acattcaaga ccttcggtta
    18061 aagcaatgct ctttttatct agactgtttt ttatataatc ccagctatct ggagcaaaac
    18121 cgagctcaaa aatatcaatt g
//
LOCUS       DAOTGZ010000030        17978 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_3064
            NODE_3760_length_17978_cov_5.403113, whole genome shotgun sequence.
ACCESSION   DAOTGZ010000030 DAOTGZ010000000
VERSION     DAOTGZ010000030.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868088
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 17978)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 17978)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:22:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,785
            CDSs (total)                      :: 2,751
            Genes (coding)                    :: 2,732
            CDSs (with protein)               :: 2,732
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 12 of 19
            Pseudo Genes (internal stop)      :: 5 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17978
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3760_length_17978_cov_5.403113"
                     /isolate="AMR_MDS_3064"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..205)
                     /locus_tag="P5123_03500"
     CDS             complement(<1..205)
                     /locus_tag="P5123_03500"
                     /inference="COORDINATES: protein motif:HMM:NF013057.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L18"
                     /protein_id="HRX15356.1"
                     /translation="MKNVSAKERRYIKRRLRVKNKIKTEDLTRKRLCIYRSNKSMYAQ
                     IIDDSEKVTLVGISTLSKEFSGKK"
     gene            complement(209..754)
                     /gene="rplF"
                     /locus_tag="P5123_03505"
     CDS             complement(209..754)
                     /gene="rplF"
                     /locus_tag="P5123_03505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018133943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L6"
                     /protein_id="HRX15357.1"
                     /translation="MSRIGKKPISIPEKVTYEISGNKITVKGPRGEDCCEFCDGLVFK
                     EEDGALCITKASDRQDDKDLNAKYGLFRSLISNIFIGVSTGFEKKLEINGVGYRVQQQ
                     GNDIQLNLGFSHPVIFKAPAGIKIEVVDQTHLTISGFNKQQVGQVAANIRELRAPEPY
                     KGKGIKYSTEIIRRKAGKAGK"
     gene            complement(779..1180)
                     /gene="rpsH"
                     /locus_tag="P5123_03510"
     CDS             complement(779..1180)
                     /gene="rpsH"
                     /locus_tag="P5123_03510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012648680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S8"
                     /protein_id="HRX15358.1"
                     /translation="MSSISDPIADMLTRIRNAVMANKSKVVVPHSNMKESIAKILKDE
                     GFIRNYKIIEDSVQNTISIQLKYTDSGESVIQHLKRKSSPGRRVYIKTEDLKPVLNNV
                     GIWILSTSKGVITNKSAKVQNIGGELLCEIA"
     gene            complement(1196..1381)
                     /locus_tag="P5123_03515"
     CDS             complement(1196..1381)
                     /locus_tag="P5123_03515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002557082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type Z 30S ribosomal protein S14"
                     /protein_id="HRX15359.1"
                     /translation="MAKESMIAKHLRGAKFKVRNHNRCAVCGRPRGFLRRFDMCRVCF
                     RKYAAEGKIPGVTKSSW"
     gene            complement(1392..1934)
                     /gene="rplE"
                     /locus_tag="P5123_03520"
     CDS             complement(1392..1934)
                     /gene="rplE"
                     /locus_tag="P5123_03520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009524371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L5"
                     /protein_id="HRX15360.1"
                     /translation="MSARLFDLYKKDVKKQLMDTFKYKSSMEIPKIEKVVLNIGMGDA
                     HANSKRLASTMDELAAITGQKPVKTVAKKSIANFKIRDGYDVGCKVTLRGDTMYEFLD
                     RLINFSLPRVRDFKGISPKSFDNFGNYNFSVKEQIIFPEIDFDKAEYIYGINITVVTT
                     GKSKEEAKALLDGFGMPFWE"
     gene            complement(1947..2267)
                     /gene="rplX"
                     /locus_tag="P5123_03525"
     CDS             complement(1947..2267)
                     /gene="rplX"
                     /locus_tag="P5123_03525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013176369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L24"
                     /protein_id="HRX15361.1"
                     /translation="MFKTKIKKEDQVVVISGSDKGKRGKVLFVDNEKNRVLVEGVNKK
                     KKHIKKAQSPNGQGGILDMEFPMKLSKVMFFCEKCKKGVRVGKEISGDNKYRICRKCG
                     KRLD"
     gene            complement(2281..2673)
                     /gene="rplN"
                     /locus_tag="P5123_03530"
     CDS             complement(2281..2673)
                     /gene="rplN"
                     /locus_tag="P5123_03530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002628222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L14"
                     /protein_id="HRX15362.1"
                     /translation="MIQMQTYLNVADNSGVKKVQCIKVLGGTNRRYATVGDIIVVAVK
                     DSHPSYGLKDSTGKKVHTKSVLKAVIVRTTKPVRRPDGSYIRFDDNAVAIIDAKNEPR
                     GTRIFGPVARELREKNFMKIVSLAPEVL"
     gene            complement(2683..2940)
                     /gene="rpsQ"
                     /locus_tag="P5123_03535"
     CDS             complement(2683..2940)
                     /gene="rpsQ"
                     /locus_tag="P5123_03535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005950435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S17"
                     /protein_id="HRX15363.1"
                     /translation="MENKKPRRQMVGKVTSNKMDKTAVVLTEKMAMHPLYKKYVKQSK
                     KIKCHDEDNTLSIGDTVRIEETRPLSKDKRFTLVEIIEKVK"
     gene            complement(2959..3159)
                     /gene="rpmC"
                     /locus_tag="P5123_03540"
     CDS             complement(2959..3159)
                     /gene="rpmC"
                     /locus_tag="P5123_03540"
                     /inference="COORDINATES: protein motif:HMM:TIGR00012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L29"
                     /protein_id="HRX15364.1"
                     /translation="MKGHLEELTSDELIKALNESKSKIQEARFKVVTGNMDNTKLIRE
                     EKKKIARILTLKKEYDLGKRKK"
     gene            complement(3172..3582)
                     /gene="rplP"
                     /locus_tag="P5123_03545"
     CDS             complement(3172..3582)
                     /gene="rplP"
                     /locus_tag="P5123_03545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011698419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L16"
                     /protein_id="HRX15365.1"
                     /translation="MLMPKKVKHRKIHRGRLKGSAQRGSSISFGEIAIKALERGKLNS
                     RQIEAARISITRKVKRGGKLWIRVFPDQPFTKKPAETRQGKGKGNPEGFEAHVLPGRI
                     LFELAGVNNEIGIAALKAAASKLPIKTKVITLED"
     gene            complement(3601..4287)
                     /gene="rpsC"
                     /locus_tag="P5123_03550"
     CDS             complement(3601..4287)
                     /gene="rpsC"
                     /locus_tag="P5123_03550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002775071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000312 - plastid small ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S3"
                     /protein_id="HRX15366.1"
                     /translation="MGQKVNPIGLRVGIVKTWSGIWFDEKKQYRKNLHEDLKIESFIK
                     KEHKNAGIAKVIIERLADKVNIHIHAARPGILIGKKGAFHEVIQANIQKLASKKVYVY
                     IVEVKKVERNSQIVAQQIAQQLEGRFPYRRAVKQAITAATRSGCKGIKVMVSGRLNGA
                     EMARSESYKEGRIPLHTLRADIDYGFAEALTTFGLIGVKVWIYNGDILSKNDAEQNQE
                     DKYAVKRKTK"
     gene            complement(4299..4634)
                     /gene="rplV"
                     /locus_tag="P5123_03555"
     CDS             complement(4299..4634)
                     /gene="rplV"
                     /locus_tag="P5123_03555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002974367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L22"
                     /protein_id="HRX15367.1"
                     /translation="METKAIAKTVRMSPMKARLVADNVRGMSFPVAVDMLSYMPNSSA
                     AIILKTLKSAGANAKNINPDLQEADLFVKKITIDEGVRLKRFRPRARGRASRIRKKTS
                     HVTVVLSDE"
     gene            complement(4651..4935)
                     /gene="rpsS"
                     /locus_tag="P5123_03560"
     CDS             complement(4651..4935)
                     /gene="rpsS"
                     /locus_tag="P5123_03560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013176376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S19"
                     /protein_id="HRX15368.1"
                     /translation="MARSVKKGPFIDIKLYKKIEEMNSSGAKKTIKTWSRRSTIFPEM
                     IGHTLMVHNGKSFIPVYVTENMVGHKLGEFSPTRNYRGHSSKNDKVAKKR"
     gene            complement(4938..5762)
                     /gene="rplB"
                     /locus_tag="P5123_03565"
     CDS             complement(4938..5762)
                     /gene="rplB"
                     /locus_tag="P5123_03565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014624190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L2"
                     /protein_id="HRX15369.1"
                     /translation="MGIKNYRPITPIQRYKTTLDYADITVDSPFKGLTKGKSQKAGRG
                     AGNQISVRRKGGGHKRKYRVIDFKRNKIDVPGKVETIEYDPNRSANIALILYKDGERR
                     YIVAPNNLSVGDEIVSGEKVEVRVGNAMPLKNIPVGTSICNVELSLGKGGQLARSAGG
                     AAQITGREGKYCMVKLPSGEMRRVLKECMATIGEIGNKDHMNVTIGKAGRSRWMNKRP
                     KVRGCAMNPVDHPLGGGEGKSAGKRHPVSPTGVPAKGYKTRKKNKYSDNMIINRRK"
     gene            complement(5783..6064)
                     /gene="rplW"
                     /locus_tag="P5123_03570"
     CDS             complement(5783..6064)
                     /gene="rplW"
                     /locus_tag="P5123_03570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005376990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L23"
                     /protein_id="HRX15370.1"
                     /translation="MNLNDVLIRPVITEKTTVQQEDGKYVFKVDRRANKVLVKQAVKS
                     LYNVDAEKVNISIVRGKKKRNKFTIGFKPSWKRAVVTLKQGDKIDLYEK"
     gene            complement(6074..6691)
                     /gene="rplD"
                     /locus_tag="P5123_03575"
     CDS             complement(6074..6691)
                     /gene="rplD"
                     /locus_tag="P5123_03575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014454653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L4"
                     /protein_id="HRX15371.1"
                     /translation="MIVDKYSVKGEKIGQVELSDKVFSDKINDVVVYEYIKAANANLR
                     QGTHKTKERAEVSGGGVKPWRQKGTGRARSGSNRSPIWVGGGTVFGPRVRSYRIELPK
                     KVKKKAFRSILSIKAQQGDIVVVEDFNVDSGKTKEMNSVLKKLNVERGIFVSSGDDTM
                     IKRSIKNIADVKYNNASRLSGRDVFYSKKVLMTESAVNAINQNFA"
     gene            complement(6709..7326)
                     /gene="rplC"
                     /locus_tag="P5123_03580"
     CDS             complement(6709..7326)
                     /gene="rplC"
                     /locus_tag="P5123_03580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013008473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L3"
                     /protein_id="HRX15372.1"
                     /translation="MKKALVGKKISMTQVYDENGVVVPVTVIELGPNVVLQKKTVEKD
                     GYSALKVGFGSIKEKNCPAPLLKDFKKKSVEPKRVLREIDAFDESLDVGSVIDCSVLD
                     GIEKVNIVSTSKGKGFAGSIKRHGFGGGRATHGSHFHRAPGSIGACAYPGEVWKGQRM
                     PGRKGSDRVTVKNLKVVKVIKDKNLVLVSGAVPGRKDSIVLVCES"
     gene            complement(7435..7752)
                     /gene="rpsJ"
                     /locus_tag="P5123_03585"
     CDS             complement(7435..7752)
                     /gene="rpsJ"
                     /locus_tag="P5123_03585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008593919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S10"
                     /protein_id="HRX15373.1"
                     /translation="MAKLAEQRIRVKLKSFDVKLLDKSAAKIVATTNRSGAKVAGPIP
                     LPTKIEKFCVLRSPHVNKTSREQFEVRTHKRLVDIVNPNSDTVEALMKLELPAGVSVD
                     IKS"
     gene            complement(7854..9074)
                     /gene="tuf"
                     /locus_tag="P5123_03590"
     CDS             complement(7854..9074)
                     /gene="tuf"
                     /locus_tag="P5123_03590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011199348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor Tu"
                     /protein_id="HRX15374.1"
                     /translation="MAKEKFDRSKPHLNIGTIGHIDHGKTTLTSAITNCMALQFGGKN
                     KAVAFENIDNAPEERERGITIATSHQEYETDVRHYAHVDCPGHADYVKNMITGAAQMD
                     AAILVVAATDGPMPQTKEHVLLARQVNVPYIVVFLNKVDMLDADERDELVELVEMEIR
                     ELLSTYEFPGDDTPIIPGSALKALEDSAAGNKDSEWVQGVVKLIKTIDEYVPEPERAT
                     DKDFLMPVEDVFSITGRGTVATGRIERGIVHTGDNVEIVGFKETASTVVTGVEMFRKL
                     LDEGRAGDNVGCLLRGTAKDDIERGQVLAKPGSITPHTDFECDVFILSKDEGGRHTPF
                     FGNYRPQFYFRTTDVTGVIVLPEGVEMVMPGDHVKKMGVQLITPVAMDETLRFAIREG
                     GRTVGAGVVSKITK"
     gene            complement(9251..11332)
                     /gene="fusA"
                     /locus_tag="P5123_03595"
     CDS             complement(9251..11332)
                     /gene="fusA"
                     /locus_tag="P5123_03595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012282468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor G"
                     /protein_id="HRX15375.1"
                     /translation="MGRDLPLHRTRNIGIMAHIDAGKTTLTERILFFTGKTHKIGEVH
                     EGAAEMDWMDQEKERGITITAAATSCMWNNTRINIIDTPGHVDFTVEVERSLRVLDSA
                     IAVFDGVAGVEPQSETVWRQADRYHIPRICFVNKMDRIGADFAYCCDMIEKKLHALPL
                     PLQIPMGAEDKFKGSIDLITMKAIYNEGDHGEKLILSDIPEEFAAEAGSMRDMMLEIV
                     AENDDELMEKYLEGSEITVDEIRSSIKKSVAERKYFPVFCGSALKDKGVQPVLDAITY
                     YLPSPKDVPPMHGHLPDSEEIVYRESSDKEKFAALIFKVRSDTYVGRLTYMRIYSGTI
                     KVGDQLFNITQNKKERIGKILRMHANNREEMQEAFAGDIVAIVGLKFAKTGDTVCDAS
                     NPVILEKMDFAEPVISIVIEPKTKVDKEKIEQALVRLEDEDPTFRVVKDEDTGQNLIS
                     GMGELHLDIIVDRLMREFNVNANVGQPQVSYKETITAETSVEKKYESVIGGKTIKAAV
                     SVSISPLQSGSGIVVESSLNKDSIASEIRDAVESGVIDGTQAGTLAMFKVDDLKIKIN
                     SCVYDDTEGAVFACRAAAGMAVRESLAKAEPVLLEPIMRVEIMSPEDYTGDIISDINM
                     RSGRIESMDMEKSIKVLHAYAPMSEMFGYATALRSMSQGRANYTMQFSRYEAVGKQKA
                     QTIINKIMGRI"
     gene            complement(11482..11952)
                     /gene="rpsG"
                     /locus_tag="P5123_03600"
     CDS             complement(11482..11952)
                     /gene="rpsG"
                     /locus_tag="P5123_03600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006831874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S7"
                     /protein_id="HRX15376.1"
                     /translation="MSRRRRPVRREVLPDPKFSSQLVTKFINVMMYDGKKGVSESIFY
                     DAMDIIKSKVDQNPVEVFEKALDNVKPLLEVKSRRVGGATYQVPVEIRPERRQALGMR
                     WLIIASRARAEKTMSQRLAAELIGAFNNTGASIKKKEDTHKMAEANKAFAHYKW"
     gene            complement(12188..12562)
                     /gene="rpsL"
                     /locus_tag="P5123_03605"
     CDS             complement(12188..12562)
                     /gene="rpsL"
                     /locus_tag="P5123_03605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012538144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S12"
                     /protein_id="HRX15377.1"
                     /translation="MPTINQLIKNGRESKGKRYKSPALKSCPQKRGVCTRVMTVTPKK
                     PNSALRKVARVRLTNGIEVTAYIPGIGHNLQEHSAVLVRGGRVKDIPGVRYHIVRGAL
                     DTLGVNDRKRGRSKYGTKRPKA"
     gene            complement(12792..17144)
                     /gene="rpoC"
                     /locus_tag="P5123_03610"
     CDS             complement(12792..17144)
                     /gene="rpoC"
                     /locus_tag="P5123_03610"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002973914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000345 - cytosolic DNA-directed RNA
                     polymerase complex [Evidence IEA];
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta'"
                     /protein_id="HRX15378.1"
                     /translation="MKDFNNFDSIKIQLASPEIIREWSYGEVKKPETINYRTLKPERD
                     GLFCEKIFGTTKEWECYCGKFKSIRYAGVICDRCGVEVTHYKVRRERSGHIELATPVA
                     HIWYYRSVPSRLGLLLNIAVSDLKKVLYYEKYVVTDPGEAEAENISRGSLLEEDEYYE
                     LYEKYGDTMIADMGAAAIKELLLDIDLDAESRNLRALILDLQANGKAIDKKLVKRVEV
                     IEAFRDSENKPEWMILDVVPVIPPELRPMVQLDGGRFATSDLNDLYRRVINRNNRLKR
                     LLALRAPDIIVKNEKRMLQEAVDALFDNSRRKRVVKGKGNRPLKSLSDMLKGKQGRFR
                     QNLLGKRVDYSGRSVIVIGPDLKLHQCGLPKRMAIELFKPFIMRRLVDKGFVPNIKSA
                     KRMVENEKPEVWEVLEEVVSEHPVLLNRAPTLHRLGIQAFEPVIVEGKAIRLHPLVCL
                     AYNADFDGDQMAVHLPLSPRAQLECWTLMLSTNNLLSPANGEPIVTPSQDIVLGLYYL
                     TAIFEDDNEKPKVYDNYDEVMMAVDRRVLKLRSKIGFYFDDEIVETTAGRLLFNKSLP
                     EGYKYINHTVGKSEIAEIIADVVRIYGATETVKLLDVIKALGYKYGTHFAPTIAISDI
                     IVPDEKKAMITAANKEIEDIENNYRMGMRTNGERFNLVVNKWTGVIDDVTNVMMKNFE
                     KAEGGFNNVYVMAKSGARGSRQQIKQLAGLRGLMAKPSGEIIDVPIRSNFKEGLNVLE
                     YFISTNGARKGLADTALKTADAGYLTRRLVDISQDVVINEIDCGTTIGIDVSAIKEGD
                     EIIESLGSRVVGRNLLYDLYHPVTNEIIAKADQIINEDTARKIDELKIDTIEIRSVLT
                     CESRHGVCAKCYGRNLASTRGVEIGEAVGIIAAQSIGQPGTQLTMRTFHIGGIATGSV
                     NETDIRFPYPLYLKNITFRTIETDDNKFVSVRRGYMEIQRNVFEYEIAKGDEILVAEG
                     EKVYPGTIIARHADKTEEISRFSGIIKLHSTKVTILGDVHSVPVNVGAVIYSHVGEFY
                     DRNEVLATVDPMMEPIITEIDGTIEFYDIEKNKSMVEETDPVSGSKKRIIVEFKTEKL
                     QPRIDVFGKSGEPTTYLLPKGAYIETEHGASVKAGAVLAKIPKEAERTKDITGGLPRV
                     AELFEARVPKDKATLAEIDGTVIIGDTVKNKRKIKIENEDESREYSIPSSKFLRVQDK
                     DFIRKGEKLDDGAIDPHDILRIKGRPELQKFLVNEVQEVYRLQGVGINDKHIEVIVRQ
                     MLRKVKISDPGDTNFVIEQIVDKFDFKFENDKVVAEGGSPASAEPVLMGITKASLNTE
                     SFISAASFQETTRVLTDAAIKAKVDNLRGLKENVIIGHLIPAGTGMPDYNNISVYQNE
                     PGDLDISEEESLEQEEAEKAAVLAAVETEATIGKEQTDEETAVKNLFKDVVADSDVED
                     DLPEPDESDDEDID"
     gene            complement(17232..>17978)
                     /locus_tag="P5123_03615"
     CDS             complement(17232..>17978)
                     /locus_tag="P5123_03615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017214121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta"
                     /protein_id="HRX15379.1"
                     /translation="HGNKGVISRIMSEYEMPYLEDGTPIDIVLNPLGVPSRMNLGQLL
                     ETELGWAAHELDLYMETPIFDGAKPYEIKEYLKKAGLPEDSKAVLYDGRTGIPFENRV
                     MTGYVYILKLAHMVDDKIHARSTGPYSLVTQQPLGGKAQFGGQRLGEMEVWALEAYGA
                     AYTLQELLTVKSDDMLGRAKMYEAIVKGHHTTSPGVPESFNVLVQELRGLALDVRIYD
                     IHGKEAVITEWGDMFGKNRKKIKLDTIKND"
BASE COUNT         5216 a         4157 c         2837 g         5768 t
ORIGIN      
        1 cctttttgcc ggaaaattct ttagaaagtg tagaaatacc aacaagtgta accttctcac
       61 tgtcatcaat tatctgtgca tacatcgact tattcgaacg gtatatacat aggcgtttgc
      121 gagtaagatc ttcagtttta attttatttt ttacacgaag acgtctttta atataacgac
      181 gctcttttgc actgacattc ttcatatttt acttacctgc cttaccagct tttcttctga
      241 ttatttctgt actgtacttg atacccttgc ctttatatgg ctctggagca cgaagctctc
      301 tgatatttgc agcaacctga ccaacctgct gtttattaaa gcctgaaata gtaagatgag
      361 tctgatcaac aacctcaatc ttgattccag caggtgcttt aaatataaca ggatgagaaa
      421 atccaagatt taactggata tcatttcctt gttgctgaac acggtatcca acaccattta
      481 tctctaattt tttttcaaat ccggtagaaa ctccgatgaa aatatttgaa ataagcgacc
      541 taaaaagacc gtattttgca ttcaaatctt tatcatcttg acgatcagat gccttagtaa
      601 tgcatagtgc accatcttct tctttgaaaa ctaaaccatc acagaactca caacaatctt
      661 caccgcgtgg tcctttaaca gtaattttat taccggatat ttcatacgta accttttcag
      721 gtatactaat cggttttttt cctatacgtg acatatttct acactcaaac tataaatttt
      781 atgctatttc acacagtaac tcgccaccaa tattctgaac tttggcagac ttatttgtaa
      841 taacaccttt agacgtagac aagatccaaa taccaacatt attaagaacc ggttttaaat
      901 cttcagtttt tatataaaca cgtcttccag gtgaactttt tctttttaaa tgctgtatta
      961 cagattcacc gctgtctgta tatttaagtt gtattgaaat tgtattctga actgaatctt
     1021 caattatttt ataatttcgt ataaatccct catctttcag aatctttgca atagattctt
     1081 tcatattgga atgaggcaca actaccttgc ttttatttgc cattacagcg tttcgaatac
     1141 gtgtaagcat atcagctatt ggatctgaaa ttgaactcat attactcctc ttatcttacc
     1201 aggatgattt cgtaacaccc ggaatttttc cttctgcagc atattttcta aaacagacac
     1261 gacacatatc aaaacgtcga agaaaaccac gaggtcttcc gcaaaccgca catctgttat
     1321 gatttctcac tttgaattta gcacctcgaa gatgctttgc tatcatactc tctttagcca
     1381 tcagtcaacc cctactccca aaacggcatg ccaaaaccat caagcaatgc cttagcttct
     1441 tctttagatt ttccagtagt aacaactgtt atattaatac catatatata ttccgcttta
     1501 tcaaaatcga tctcggggaa aataatctgt tctttaacag aaaaattata gttaccaaaa
     1561 ttatcaaacg actttggaga aataccttta aagtcacgaa cacgaggaag cgagaaattt
     1621 attaatcgat ctaaaaactc gtacatggta tcaccacgca aagtgacctt gcaacctaca
     1681 tcatagccat ctcgtatttt gaagtttgca atcgactttt ttgcgacagt tttaacaggc
     1741 ttttgtccag ttatagcggc caactcatcc atagtcgaag caagtcgttt agaatttgca
     1801 tgggcatcac ccataccaat attaagtaca actttttcga tttttggaat ttccatcgaa
     1861 gatttatact taaaagtatc cataagctgc ttttttacat cttttttata aagatcaaat
     1921 aatcttgctg acatctcaat taccctttag tccaatcttt ttccgcattt tcggcaaata
     1981 cggtatttat tatcgccaga aatctcttta ccaacacgga cgcctttttt gcatttctcg
     2041 caaaaaaaca ttactttcga gagtttcata ggaaattcca tatcaagtat cccgccctga
     2101 ccgttaggtg actgagcttt tttgatatgt tttttctttt tgttaacgcc ttcgacaaga
     2161 actcgatttt tttcattatc aacaaaaaga acttttcctc ttttaccctt atctgaacca
     2221 gaaataacaa caacctggtc ttcttttttt attttagtct taaacatatt attaatcctc
     2281 ttaaagtact tcgggagcca aagaaactat tttcataaaa ttcttctcac gcaattccct
     2341 tgcgaccggt ccaaagatac gtgtgccgcg aggttcattt ttggcatcaa ttatcgcgac
     2401 agcattatca tcaaaacgga tataagaacc atcaggacga cgcacaggtt tggtagtacg
     2461 aacaattact gcctttagaa cagatttggt gtgtactttt ttaccagtac tgtcttttaa
     2521 accataagac gggtgagagt cttttactgc aacaacaata atatcaccaa ccgttgcgta
     2581 tctacgatta gtaccaccaa gtactttgat acactgtact ttttttacac cgctgttatc
     2641 agcaacattc aggtatgtct gcatctgtat catgataaat ccctacttaa ctttttcgat
     2701 tatttcaaca agagtaaaac gtttatcctt ggatagcggt cttgtctctt cgatacgaac
     2761 tgtgtcacca attgacaaag tattatcttc atcgtgacat ttaattttct tactctgctt
     2821 aacatatttc ttataaagag gatgcattgc catcttctcg gtaagaacaa cagctgtttt
     2881 atccatttta ttactggtga ctttacccac catctgtctt cgtggctttt tgttttccat
     2941 caaaaattac ctgctctatt actttttacg ttttccaaga tcatactctt ttttaagagt
     3001 gagtattcgc gcaatctttt ttttctcttc acgaattaac ttggtattat ccatgttgcc
     3061 agttacgact ttaaatcggg cttcctgaat cttagacttt gactcattaa gcgcctttat
     3121 tagctcatca ctggttaatt cttcaaggtg tcctttcata ttaaaaacct attaatcttc
     3181 aagtgttata acttttgttt taattggcag tttagaagct gcggctttca aagcagctat
     3241 accaatctca ttgtttacac cggcaagttc aaaaagaatt cgacctggta aaacatgagc
     3301 ttcaaaacct tccggatttc ccttaccttt tccctgacga gtttcagcag gtttttttgt
     3361 aaaaggttga tcaggaaaaa ccctaatcca taattttcca ccacgcttta ccttacgtgt
     3421 aatagaaata cgagcagcct caatctgacg gctattaagt tttccgcgct ccaaagcctt
     3481 aatggctatc tcgccaaaag aaattgaaga acctcgttga gcggatccct ttaggcgacc
     3541 gcgatgtatc tttctatgct taactttttt aggcattaac attatttata accctaacca
     3601 ttactttgtc ttgcgtttta ctgcgtattt atcttcctga ttttgctcgg catcattttt
     3661 tgaaagaatg tctccgttat aaatccagac tttaactcca atcaaaccga aagttgtcaa
     3721 agcttctgca aaaccgtagt caatatcggc tctaagtgta tgaagaggaa ttcgcccctc
     3781 cttgtacgat tcacttctag ccatttcggc tccgtttaat cgaccagaca ccataacctt
     3841 tattcccttg caaccggaac gtgtggcagc agttattgcc tgcttaactg ctcttctata
     3901 aggaaaacgt ccttcaagct gttgtgcaat ttgttgagca acaatttgag aattacgctc
     3961 aacttttttt acttcaacaa tataaacata aacttttttt gacgcaagct tctgaatatt
     4021 tgcctgaata acctcgtgaa aagcaccttt ttttccgata agaattcctg gacgcgcagc
     4081 gtggatatga atatttacct tatcggcgag tctttcaata ataaccttcg ctataccggc
     4141 attcttatgt tcttttttaa taaaagattc aatctttaga tcttcatgaa gattttttcg
     4201 gtactgtttc ttttcatcaa accagatacc gctccatgtt ttaactattc cgaccctaag
     4261 tccaattgga tttacttttt gtcccatgaa atattccttt attcgtctga taatactaca
     4321 gtaacatgac tggttttctt tcgtattctt gatgcacggc cacgggcacg cggtctaaac
     4381 cgctttagtc ttacaccttc atcaatcgta atcttcttaa cgaaaagatc tgcttcctga
     4441 agatcaggat taatattctt tgcgtttgct ccggctgact taagtgtttt aagaatgatc
     4501 gcagcagaac tgttaggcat atatgataac atatccacag cgaccggaaa agacataccc
     4561 ctaacgttat cggccactaa tcttgccttc ataggagaca tgcgcaccgt ttttgctatt
     4621 gcttttgttt ccatcttcta taatcccaat ctatcttttt ttagcaactt tatcattttt
     4681 acttgaatgg ccacggtagt ttctcgttgg agagaattct cccagtttgt gaccaaccat
     4741 attctctgta acatataccg ggatgaaact tttcccattg tgaaccatta aagtatgacc
     4801 gatcatttca ggaaaaattg ttgaacgtct cgaccaggtc ttaatagttt ttttagcccc
     4861 agaagaattc atctcttcaa ttttcttata gagctttatg tcaataaatg gtcctttttt
     4921 taccgatcta gccatattta cttccttcta ttgataatca tattatcact gtatttattt
     4981 ttctttcgag tcttgtaacc tttagcaggt acacctgtag gagaaacggg gtgtctcttt
     5041 ccagcactct taccttcacc accaccaaga gggtgatcta cagggttcat tgcgcaacca
     5101 cgaacttttg gtcttttatt catccaacgt gaacggccag ctttacctat agtaacattc
     5161 atatgatcct tgttacctat ttcaccgata gtagccatac actctttaag aactcgtctc
     5221 atttcgcctg aaggaagctt gaccatacaa tactttcctt cacgaccagt aatctgagca
     5281 gctccaccag cagaacgcgc cagctgtcca cccttaccaa gactaagctc aacattacag
     5341 atactagtac caactggaat gttttttaga ggcatagcat ttccaactct tacttcaacc
     5401 ttttcacctg agacaatttc atcaccgaca gaaagattat ttggagcaac tatatatctt
     5461 ctctctccat ccttgtaaag gataagagct atattagcag aacgatttgg atcatactcg
     5521 atagtttcta ctttaccagg aacatcaatt ttgtttcttt tgaaatctat aacccggtac
     5581 ttacgcttat gcccgccacc ttttctacga acagaaatct gattcccggc accacgtccg
     5641 gctttctgag atttaccttt ggttaaaccc ttaaatgggc tatctacagt aatatctgca
     5701 taatcaagag tagtcttata tctctgaatc ggagtaattg gtctgtaatt tttaattccc
     5761 attgctatac cctaatgact gattattttt catacaaatc aattttgtct ccctgtttaa
     5821 gcgtaactac agcacgcttc cacgagggct taaaaccgat tgtaaactta tttctctttt
     5881 ttttaccgcg aacgatagat atgttaacct tttcagcatc aacattatac agacttttta
     5941 ccgcctgttt taccaaaact ttgtttgctc gacgatctac tttaaaaaca tactttccat
     6001 cttcttgttg aacagttgtc ttttcggtaa tcaccggacg tattaaaaca tcattcaaat
     6061 tcatcatcat accctatgcg aaattctgat ttatagcatt tacagcactt tctgtcatca
     6121 acactttttt agaataaaaa acgtcacgac cagaaagtct agaagcattg ttatatttaa
     6181 catcagcaat attttttatg gatctcttaa tcatggtatc atctccagaa gagacaaaaa
     6241 tacctctttc gacattaagt ttctttaaga cagaattcat ctctttggtt ttacccgaat
     6301 ccacattaaa atcttcaaca acaacgatat ccccttgttg agctttaatg gacaatattg
     6361 aacggaaagc ctttttcttt accttttttg gaagctcgat acggtacgaa cgcactcttg
     6421 gtccaaaaac ggtaccacca ccaacccaaa ttggagatcg atttgatcct gaacgcgcac
     6481 gtccagtacc tttttgacgc caaggcttaa ctccaccacc gctaacttca gctctctctt
     6541 tagtcttatg agttccctgc cgaagattcg cattagcagc ctttatatac tcatagacaa
     6601 caacatcatt aatcttatcc gaaaaaacct tgtcagacaa ttctacctga ccaatttttt
     6661 caccttttac agaatattta tctacaatca tcttagtact ccaccttgtc agctttcaca
     6721 aacaagaaca attgaatctt tgcgaccggg aacggcaccg gatacaagaa caagattctt
     6781 atccttgata acttttacaa ctttaagatt ttttacagta acgcgatcag agccttttct
     6841 tccaggcata cgctgacctt tccagacttc acccggatag gcacaagctc caattgaacc
     6901 gggagcacgg tgaaaatgcg aaccgtgtgt cgcacgacca ccaccaaaac catgtctctt
     6961 aatacttcca gcaaatccct tacccttgga tgtcgaaaca atatttactt tttcaattcc
     7021 atccaaaaca ctacaatcga ttactgaacc gacatcaaga gattcatcaa aagcatctat
     7081 ttcgcgaaga acacgtttag gctcgacact cttcttttta aagtctttta ataaaggagc
     7141 aggacagttt ttttctttaa tagatccaaa accaaccttt aacgccgaat atccatcttt
     7201 ttcgacagtc tttttttgta acacgacatt gggaccgagc tctataacag tcaccggcac
     7261 aactacgcca ttttcatcgt agacttgagt catgctaatt tttttcccaa ctaacgcctt
     7321 tttcatcaca tttttcccat atataattga ttatacaccg gccatggtca acaagaaccg
     7381 ggtaacgata tcaacacaat tctcaaaact attttagaga tactacggca attcttacga
     7441 cttaatatct acagaaacac cagcaggaag ttcaagcttc atcaacgctt caaccgtatc
     7501 agagtttgga ttaactatat caaccaaacg cttgtgagtt ctaacctcaa actgctcacg
     7561 agaagtctta ttaacatgcg gcgaccgcaa tacacaaaac ttttcaattt tagtaggtag
     7621 cggaatagga cctgcgacct tcgctccact tctatttgtt gttgcaacaa tctttgctgc
     7681 cgatttatca agtaacttta catcaaaaga ctttagtttt accctgattc tctgttccgc
     7741 taacttagcc acgtttttcc tcaaattgta aaattattat tagttgccag gccttatctg
     7801 ttcagcctga caactattat tatacggcat ttattcgtca aataaatttt aaattatttt
     7861 gttatttttg atacaacacc tgcaccaaca gttcttccac cttcgcgaat cgcaaaacga
     7921 agagtctcgt ccattgctac cggagtaata agttgaacac ccatcttttt aacatggtca
     7981 ccaggcataa ccatctctac tccctcagga agtacgatta caccggttac atccgttgta
     8041 cgaaagtaaa actgtggacg ataatttccg aagaaaggag tatgtcgacc accctcatct
     8101 tttgaaagga tgaaaacatc acattcaaaa tctgtatgtg gagtaataga acccggctta
     8161 gcaagaacct gaccgcgctc gatatcatcc tttgcagttc cacgcaaaag acaaccaacg
     8221 ttatcacccg ctctaccctc atcaagaagc ttcctgaaca tctctacacc agtaacaaca
     8281 gtagaagcag tctctttgaa accaacgatt tcaacgttat cgccagtatg gacgattcca
     8341 cgctcaatac gtccagttgc aacagtacca cgtcctgtaa ttgagaaaac atcctcaaca
     8401 ggcataagaa aatccttatc agttgcacgc tctggttctg gcacatactc atcaatggtt
     8461 ttaataagct taacaacgcc ctgaacccat tctgaatctt tgtttcctgc agctgaatcc
     8521 tcaagagctt taagggcaga accaggaata atcggagtat catctccagg aaattcgtaa
     8581 gtacttaata actcacgtat ctccatctcg acaagctcta ccagctcgtc acgctcatca
     8641 gcatcaagca tgtcaacctt attaagaaat acaacaatat aaggtacgtt tacttgacga
     8701 gcaagaagaa cgtgctcttt tgtttgcggc attgggccat cagtagcagc aactacaaga
     8761 attgcagcat ccatctgagc ggcacctgtt atcatgtttt ttacatagtc ggcgtgtcca
     8821 ggacagtcaa cgtgtgcata gtgacgaaca tctgtctcgt attcctggtg agaagtagcg
     8881 atagtaatac cacgctctct ctcttctgga gcattatcaa tattttcaaa tgcaacagct
     8941 ttgttttttc cgccgaattg aagagccata cagtttgtga ttgccgaagt aagagtagtc
     9001 ttgccgtggt caatatggcc aatcgttcca atgtttaagt gtggcttgga tctgtcaaat
     9061 ttttcctttg ccatgtgaat tcacctcttt tattacttga atacaaatct attattaatt
     9121 tatttaatag caatagtgac ctgaaagtaa caaaattaaa aattatatgc cttttgtgtc
     9181 aacaaaatta atccattaat cttaattaat cgaaatatta gaaaaaaaag atcaaaatgt
     9241 cacaagatta tcatatccgc cccataatct tgtttattat agtttgcgct ttctgtttac
     9301 caactgcttc atagcgacta aactgcatag tgtagttggc tctaccctgg ctcatggatc
     9361 ttaatgctgt agcatatccg aacatttcag acattggcgc ataagcatgc aatactttaa
     9421 tgcttttttc catatccata ctctcgattc gtccagaacg catattaata tcacttatta
     9481 tgtctccagt atagtcttca ggtgacataa tctcaacacg cataataggt tcgagcagaa
     9541 ccggttcagc ttttgcaaga gattctctaa cagccatacc ggccgcagcc ctgcatgcaa
     9601 acacagcacc ctcggtatca tcatatacac atgagttaat cttaattttt aaatcatcta
     9661 ccttgaacat tgccaaggta ccagcttgcg ttccatctat cactccagat tcaacagcat
     9721 ctctgatctc ggaagctata ctatctttat ttaaggatga ttcaacaaca atgcccgagc
     9781 ctgattgcaa cggagaaata gagaccgaaa ccgcagcctt aattgtttta ccgccaatca
     9841 cactttcata tttcttctcg acagaggtct cggcagtaat tgtctcttta tacgatacct
     9901 gcggttgacc aacattggca tttacattaa attcgcgcat taaacgatct acaataatgt
     9961 ccaggtgcag ctcacccata cctgatatta gattttgacc cgtatcttca tctttgacaa
    10021 cacgaaaggt cggatcttca tcttctagcc gaacaagtgc ctgctcaatt ttttctttat
    10081 caacttttgt ttttggttca attacaattg agataacagg ctccgcaaaa tccatttttt
    10141 caagaataac gggatttgac gcatcacata cggtatcacc ggtttttgca aattttagac
    10201 ctacaatcgc aacaatatca ccggcaaaag cctcctgcat ctcttccctg ttattggcat
    10261 gcattctcag tattttgcca attctctctt ttttattctg tgtaatattg aaaagctgat
    10321 caccgacctt aattgttcct gaataaattc tcatgtatgt taatcggcca acatatgtat
    10381 ccgatcgaac cttaaatatc aaagcagcaa acttttcttt atctgaggat tcgcgatata
    10441 ctatctcttc agaatctgga aggtgcccat gcattggagg cacatccttt ggagaaggaa
    10501 gatagtacgt tatggcgtca agaactggct gcacaccctt atcctttagc gctgaaccgc
    10561 aaaatactgg aaaatatttt ctttcagcca cagatttttt tattgaagaa cgtatctcat
    10621 caactgttat ttctgatcct tccagatatt tttccataag ctcatcatca ttttcagcaa
    10681 caatttcaag catcatatca cgcattgagc ctgcctcagc agcaaattct tcaggtatat
    10741 cagataaaat caacttttcg ccatgatcac cttcattata tattgctttc atggttatca
    10801 agtctattga gcccttaaac ttgtcttcgg ctcccattgg tatctgaagc ggcaaaggta
    10861 acgcgtgcaa ttttttttct atcatatcgc aacaatatgc aaaatcagca ccgattcgat
    10921 ccatcttgtt tacaaaacat attcttggaa tgtggtatct gtctgcctgc cgccaaactg
    10981 tttctgattg cggctctacg ccagcaacac cgtcaaaaac tgctattgcc gaatcaagaa
    11041 cacgtaaaga cctttcaacc tctacggtaa aatcgacatg tccaggtgta tctattatat
    11101 taattcgcgt attattccac atgcagctcg ttgcagcagc cgtgattgta atacctcgct
    11161 ctttctcctg atccatccag tccatttcgg ccgcaccctc atgaacttcg ccaatcttat
    11221 gggttttccc agtaaaaaac aagattcttt cggttaacgt ggttttcccc gcatcaatat
    11281 gtgccattat acctatgttt cttgtccgat gaagtggtag atctcgtccc ataaatcctc
    11341 ttaaatcaca atcctaataa taaaaacacc tgtctctgca gtagctgaaa caggtgtgtc
    11401 catataatac ataaaggtta tactgggtaa aacaaagaaa ctatgaaacc cagattaacc
    11461 aaatacttaa agctgatatt attaccattt ataatgagca aaggccttat ttgcctcagc
    11521 cattttatgt gtatcctctt ttttcttgat cgaagcacct gtattattaa aagctcctat
    11581 caactcggca gcaagacgct gagacattgt tttctcagct cgtgctctag aagcaatgat
    11641 cagccacctc atcccaagag cttgtcttcg ttctggacga atttcaactg gaacctgata
    11701 agtagcacca ccaactcgac gagattttac ttcaagaagt ggcttgacat tatcaagagc
    11761 tttctcaaaa acctcaacag gattctgatc gaccttcgat ttgattatat ccatcgcatc
    11821 ataaaatata ctttctgata cacctttttt accatcatac atcataacat ttatgaattt
    11881 ggtaactagc tgagaactaa atttcggatc aggcagaacc tctctgcgta ccggtcttct
    11941 tcttcgcgac ataatattcc tctttaacag acagcatcaa tagaacaaat gaataaaaat
    12001 tcttgttaaa cttacgctgt aaaagttata atattttcta attataaata aagacaaagt
    12061 aaacccggat gcaaagccat cctgattctc cataaacaat aaaagacaac cgaaacccgc
    12121 tcccggaaga tgcaactctt tccctggatt actcagcatg tacgtgtctc agttatgcaa
    12181 ttagagctta agcctttggt ctttttgtac catatttaga acgaccacgt ttacgatcat
    12241 tcaccccaag cgtatcaaga gcgccacgaa caatgtgata acgcacacct ggaatatcct
    12301 ttacccttcc accacgaaca agaactgcgg agtgttcctg aagattatga ccaattccag
    12361 gaatatacgc tgtaacctct attccgtttg ttaaacgaac acgggcaact tttcttaacg
    12421 ctgagttagg tttttttggt gttacagtca taacacgcgt acaaacccca cgtttttgcg
    12481 gacatgattt taatgcaggc gacttgtaac gtttaccctt actttctcgt ccatttttta
    12541 ttagctgatt gattgttggc attttatatc ctcataaaaa ttatatatca attattgaaa
    12601 acctaacatc ttagatagat gcttaaaaac cattaagtca aaattggtca ggttatgact
    12661 atatcttcgt agagccgtta attgtcaaga aaattaatgc aaaagctcat cgccagcagt
    12721 cgcatcatga cagatattaa ccgaaaacaa ctttagaccg aaacatcatt ttgatatttc
    12781 ggtctattgt attaatctat atcttcgtcg tcagactcat ctggttcggg aagatcatct
    12841 tcaacatctg aatcggcgac tacatctttg aaaagattct taacagcagt ctcttcatct
    12901 gtctgttctt ttcctattgt agcttcagtt tcaaccgcag ctaaaactgc tgctttttcg
    12961 gcctcttcct gctccaggct ctcttcctca gaaatatcaa gatcacccgg ttcattctga
    13021 taaacagaga tattgttata atcaggcata cctgtaccag ccggtataag atgaccaata
    13081 attacatttt cttttaaacc tcggagatta tcaacctttg ccttgatagc tgcatcagtt
    13141 aatactcttg ttgtctcttg gaacgaagct gcagaaataa aggactcggt attaagcgat
    13201 gcttttgtta tacccatcaa aacaggttct gccgaagcag ggctaccacc ttccgcaaca
    13261 actttgtcat tctcaaattt aaaatcaaac ttatcaacta tctgttcgat tacaaaattt
    13321 gtatcaccag gatcagagat tttaacctta cgaagcatct ggcgaacaat aacctctata
    13381 tgcttatcgt taatgccaac accctgtaaa cgatatacct cctgaacctc atttacaaga
    13441 aacttttgaa gttctggacg acctttgatt cttaaaatgt catgagggtc aatcgctcca
    13501 tcgtcaagtt tttccccttt tcggataaaa tctttatcct gtactcggag aaatttggat
    13561 gatggtatag aatactcgcg gctctcatcc tcattctcaa ttttaatttt tcgcttattc
    13621 ttaacggtat ctccgatgat aactgttcca tcaatttcgg caagagttgc cttatctttt
    13681 ggtacgcgag cctcgaacaa ttcggcaaca cgtggtagac cacctgtaat gtcttttgtt
    13741 ctttcagcct cttttggtat ttttgcgaga acagcacctg ctttaacaga agcaccatgc
    13801 tcagtttcaa tataagcccc tttcggcaac agataagttg ttggttcacc agatttacca
    13861 aatacatcta tacgcggttg caatttttct gtcttaaact caacaataat ccgcttttta
    13921 gatccactga cagggtctgt ctcttcaacc atactcttat ttttttcaat gtcatagaac
    13981 tctatggtac catcaatttc ggttataata ggttccatca tagggtcaac cgtagccaaa
    14041 acttcattac ggtcatagaa ttctccaaca tgagaatata tcaccgcacc tacgttaact
    14101 ggaacagaat ggacatctcc aagaatagta accttggttg agtgcagttt gataatacct
    14161 gaaaaacgcg aaatctcttc ggttttatcg gcatgtcttg caataattgt tcccgggtat
    14221 actttttcac cttctgcaac aagtatttca tcacccttgg caatttcgta ctcaaaaaca
    14281 ttacgctgaa tttccatata accacgacgt actgaaacga acttgttatc atcggtttca
    14341 attgtgcgga atgtaatatt tttaagatat aacgggtatg ggaatcgtat atcagtttcg
    14401 ttaacagaac ctgtagcaat acccccaatg tgaaatgttc tcattgtaag ctgggttcca
    14461 ggctgcccta ttgattgtgc cgcgataatt cctaccgctt cccctatttc cacacccctt
    14521 gtcgaagcaa ggtttcttcc gtaacattta gcgcatacac catgacgtga ctcacaagtt
    14581 aaaacagaac gtatttcaat tgtatcaatt tttaactcgt caatctttct ggcagtatct
    14641 tcatttatta tttgatcagc tttagcgata atctcatttg taacaggatg ataaagatca
    14701 taaagaagat ttcttccaac aactcgacta ccaagagatt cgattatctc atcaccctct
    14761 tttatcgccg atacatcgat acctatagtt gttccacaat caatctcgtt aataacaaca
    14821 tcctgtgata tatctacaag tcgtcttgta aggtatccgg catccgcagt ttttagggct
    14881 gtatcagcaa gtcctttacg agcaccgttt gtcgaaatga aatattcaag aacattcaat
    14941 ccttctttaa agttagatcg aataggtaca tcaataattt cgccactcgg ttttgccata
    15001 agtccgcgta atcctgccaa ctgcttaatc tgttgacgac tacctcgagc acccgactta
    15061 gccattacgt aaacattgtt aaaaccacct tcggcttttt caaagttttt catcataacg
    15121 ttggttacat catcaataac accagtccat ttgtttacaa ccaggttaaa acgttcacca
    15181 tttgtacgca tacccattct ataattgttt tctatgtctt ctatctcttt gtttgcagct
    15241 gtaatcatcg cctttttttc atctggaaca ataatatccg atattgcgat tgtcggagca
    15301 aaatgcgttc catacttgta accaagagct ttgattacat ctagaagctt aacagtctct
    15361 gttgcaccgt aaatacgaac tacatcagcg attatctcgg ctatttcact tttcccgaca
    15421 gtatgattta tgtatttgta accttcagga agcgacttgt tgaaaagaag tcgtcctgcc
    15481 gttgtttcta caatttcatc gtcaaaatag aagccaatct ttgaacgcag ttttaaaaca
    15541 cgacggtcaa cagccatcat aacttcatcg tagttatcat aaacctttgg tttctcattg
    15601 tcatcttcaa atattgctgt aagataatac aatccaagaa ctatatcctg tgaaggcgta
    15661 acaataggtt caccgtttgc aggcgataga agattgtttg tagagagcat aagagtccag
    15721 cactcaagtt gggcgcgcgg agataaaggc aagtgcacag ccatctgatc gccatcgaag
    15781 tcggcattat aagcaagaca aaccaaagga tgcaaacgta ttgcttttcc ctctacgatt
    15841 accggctcaa aagcctgaat accaagacgg tgcaatgtcg gagctctatt aagcaataca
    15901 ggatgttcag aaacgacctc ttccagaact tcccatactt caggtttctc gttttcgacc
    15961 atacgtttag ccgattttat attgggcaca aaccccttat caacaagacg gcgcataatg
    16021 aaaggcttaa aaagttcgat tgccatccgc ttaggaagtc cacactgatg tagtttcaaa
    16081 tcgggaccga taacaataac cgaacgaccg gaataatcga cacgtttacc cagaaggttt
    16141 tgcctaaaac gcccctgctt acctttaagc atatctgaaa gtgatttaag aggtcggttt
    16201 ccctttcctt ttacaactct cttgcgtcgg ctgttatcaa ataacgcgtc tacagcctct
    16261 tgaagcatac gtttctcatt ttttacaatg atatctggag cacgtagagc caataaccgt
    16321 tttaagcggt tattacggtt aattactcga cgataaaggt cattaagatc cgaagttgcg
    16381 aaacgtcctc cgtcaagctg caccatcgga cgaagctctg gtggaattac aggtacaaca
    16441 tcaagtatca tccactcagg tttgttttcg gaatctctga aagcctcgat aacctcaact
    16501 cgcttaacaa gttttttatc gatagcttta ccattcgcct gtagatccaa tatcagtgcc
    16561 cgcagattac gtgattcggc atcaagatct atatcaagaa gcagctcctt tattgctgct
    16621 gctcccatat cggcgatcat cgtatcacca tatttttcgt agagctcgta atattcatct
    16681 tcttcgagca aagatcccct gcttatgttt tcagcctcgg cctcacctgg atctgtcact
    16741 acatattttt cataatacag cactttttta aggtcactaa ccgctatatt aagcagcagt
    16801 ccgagccttg aaggtactga acggtaatac catatatgag ctaccggtgt tgcaagttca
    16861 atatgaccac ttctttcacg acgaacctta tagtgtgtta cctcaacacc acaacggtca
    16921 caaattacac ccgcgtaacg gatcgattta aacttaccac agtaacactc ccactccttt
    16981 gtggtaccga atattttttc acaaaataga ccgtcccttt caggtttaag tgttctgtag
    17041 ttaatagttt ccggcttttt aacttcacca tacgaccatt ccctgatgat ttccggtgaa
    17101 gcaagctgta tcttaattga atcaaagtta ttaaaatctt tcatcttcta tccttgatct
    17161 ttttatagga taatactgat tactgctatt atttcactgc cattcatgaa aaagcaatgg
    17221 cagatcaatt cttaatcatt cttgatagta tcaagtttaa tttttttgcg atttttaccg
    17281 aacatatcac cccattcggt aattactgcc tctttgccgt ggatatcata aatacgcaca
    17341 tccaacgcaa gaccgcgaag ctcctgaaca agtacattaa aagattctgg aacccccgga
    17401 gaagttgtat ggtgtccttt aacaattgcc tcgtacatct tcgcgcgacc aagcatatca
    17461 tcggacttaa cagttaaaag ctcctgtagt gtatacgcag cgccgtaagc ttcaagagcc
    17521 caaacctcca tctctcccaa acgctgacca ccaaattggg ccttaccgcc aagaggctgc
    17581 tgtgttacaa gagagtatgg ccctgttgag cgcgcgtgaa ttttatcatc aaccatatga
    17641 gctagtttaa gaatataaac ataacccgtc attacacggt tttcgaaagg aattcccgta
    17701 cggccatcat aaagtaccgc ttttgaatct tcaggaagac ctgctttttt aagatattcc
    17761 ttgatttcat acggtttagc accatcaaaa ataggagttt ccatatagag atcaagttca
    17821 tgagctgccc aaccaagctc ggtttcaagc aactgtccta gattcatacg agacggtaca
    17881 ccaagaggat taagcacgat gtcaattggt gttccatcct ccagataagg catctcatat
    17941 tcagacataa tgcgggaaat aacacccttg tttccatg
//
LOCUS       DAOTGZ010000031        17879 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_3064
            NODE_3791_length_17879_cov_4.949338, whole genome shotgun sequence.
ACCESSION   DAOTGZ010000031 DAOTGZ010000000
VERSION     DAOTGZ010000031.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868088
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 17879)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 17879)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:22:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,785
            CDSs (total)                      :: 2,751
            Genes (coding)                    :: 2,732
            CDSs (with protein)               :: 2,732
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 12 of 19
            Pseudo Genes (internal stop)      :: 5 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17879
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3791_length_17879_cov_4.949338"
                     /isolate="AMR_MDS_3064"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1599)
                     /gene="pilM"
                     /locus_tag="P5123_03620"
     CDS             complement(<1..1599)
                     /gene="pilM"
                     /locus_tag="P5123_03620"
                     /inference="COORDINATES: protein motif:HMM:NF022554.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pilus assembly protein PilM"
                     /protein_id="HRX15380.1"
                     /translation="MFEKLLAIDIGTSSIKALSAKRGFKSNEIIHFSHRMINPESADQ
                     QAEIEEALTDLMSEFTDNDYLLITNIPLKSVILRNFSFPFSEKSKISEILPMEAEETL
                     PYDLDDIDISFQLANEGTEEGKIIAAAIQKEHLNQFKVVIGTTGITPHYIGIESNSLF
                     ESLLPETLQFDSFLQIDIGHSKTTVNLVHRKKIAATRLIPGGCSSIISKMASILKINY
                     YEAFGKFENLHLDLSDIENNYSSNFYKSLKITKKQLQEIYDFSILEFESIIDQIRLTI
                     QAFTAANHSVDFERIYLSGGASLLPRISDFFANAMEIPVGSLPNVQLPNQIAQARFSS
                     CYGLIELYRNKTDYINFCENHKNSSFGSIQIEKLYPALFFTSLSAIMLIIVLILNLFF
                     TSREKALTRDLLQKQYTTLFKSQPTDDDPIMSANKLLKKELDKLNQIRNLIPEENSTI
                     ELISAISAQMPEESDIEIQSLVISDKDIKMSGTVETSSTLSDFMTKLKSVSLFESAID
                     RTTSTGSISRFSIEIKLKQNTAKKG"
     gene            complement(1727..3010)
                     /locus_tag="P5123_03625"
     CDS             complement(1727..3010)
                     /locus_tag="P5123_03625"
                     /inference="COORDINATES: protein motif:HMM:NF015867.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system protein GspK"
                     /protein_id="HRX15381.1"
                     /translation="MRSLRLHHCLTGTNRMINIKKHISQIRQMYRQRAAKLEKHGLFG
                     SNEKNRGFVLIIVLIVSTLLISVSTDFLVSAQNNINYMIKFKNEAQAEAAAQAALNIA
                     HIILDIDQKGVTPPMIPVKNKDKNIDTFGDIWAFDFPEFELANATVKIIIEDEQSKIN
                     LSAIATEYVPQTTFFGIINRFFMNMGFPMDYADAVADWVDRDNSRTGNGAETYDHYST
                     LQTPYSAANAPLESIDRLLLVRYFSPAIYYGFSGGNMAQEKEEGMLVESNDEIMSLDV
                     LSIMESVSDDSEETTEEEESGESEEEDIPIGPESSRRLDKYLRVNGDPKLFNSQVNKI
                     NINTAPYRVLRSIAPDMTDNDAKQIILRRRINPFKKVDDVKEFIRDDLIRRNCLTVNS
                     NLFRITVHAYVNRHKVTITAIYDRSLKYYSYYALH"
     gene            complement(2967..3641)
                     /locus_tag="P5123_03630"
     CDS             complement(2967..3641)
                     /locus_tag="P5123_03630"
                     /inference="COORDINATES: protein motif:HMM:NF023044.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin-type N-terminal cleavage/methylation
                     domain-containing protein"
                     /protein_id="HRX15382.1"
                     /translation="MKKFIKRLKSNEGFTLLELLVAVTISSIVMMMIYGANRTIMFAI
                     HDMSGIADFYENTNMAIRRMDRDLSCTLQDRYNKELFLVGENNPYPPYMGKINMVTVV
                     KSEIYVAGSYSEPAFNSDIKEISYFLKPDPSYPDMFFLMRKESIEYNKDDENKPQGID
                     SILLENVIDMRIEYTEDKINDWSQNWDSRDRNRFPFGIRVTLTLRNYRGQDEIFTFTS
                     LLNRYQ"
     gene            complement(3638..4036)
                     /locus_tag="P5123_03635"
     CDS             complement(3638..4036)
                     /locus_tag="P5123_03635"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin-type N-terminal cleavage/methylation
                     domain-containing protein"
                     /protein_id="HRX15383.1"
                     /translation="MTSNQCGNPVFNKLGFALIEILIAVTILSLVVLPLYKGISTGSM
                     AISQNKNLTKAIVIARSKLNELKIQKMQNNVIAGSEVPEYPDFTYEISIEKFEHELLG
                     PIPANIAVVTVFWMENNAERSYSIEYIYPL"
     gene            complement(4002..4601)
                     /locus_tag="P5123_03640"
     CDS             complement(4002..4601)
                     /locus_tag="P5123_03640"
                     /inference="COORDINATES: protein motif:HMM:TIGR02532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system protein"
                     /protein_id="HRX15384.1"
                     /translation="MRILPAGNNRKQSNQGFSLIEILIVIALLSIVSIVIVPRVSNIF
                     DNSQKNLSLVSSMVVKTFDDAFINNRINFLAVHLYFTSVTEFDDQNKEILERSNGISV
                     VNVSPEGLLIDSQNKMLKPRQFSSGFMINRVLLDDGSDISSGTALIPFYPDGFSKNAI
                     IHVTLNDTERYSIIISKLMKYPYTKEGHIDFESMWKSGF"
     gene            complement(4576..5055)
                     /gene="gspG"
                     /locus_tag="P5123_03645"
     CDS             complement(4576..5055)
                     /gene="gspG"
                     /locus_tag="P5123_03645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010940994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015627 - type II protein secretion
                     system complex [Evidence IEA];
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system major pseudopilin GspG"
                     /protein_id="HRX15385.1"
                     /translation="MLILKKLFKHISTVIKKLKARDGFSLLEIMIAVTIMSILAIAIV
                     PQFMDMPDKAKVSKANEMIKSLELSLNLYMMDNGTYPTTDQGLAALIEAPTSEPVPNN
                     YKTGGYIQSSSVPKDPWKNDFIYVSPGEEGRPYEIICLGADNQEGGEGVNADITSWE"
     gene            complement(5216..6469)
                     /locus_tag="P5123_03650"
     CDS             complement(5216..6469)
                     /locus_tag="P5123_03650"
                     /inference="COORDINATES: protein motif:HMM:NF012694.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system F family protein"
                     /protein_id="HRX15386.1"
                     /translation="MIYQYEALNRRGERLSGIVDATSETKAKLMLKQEGLYVSKIGPK
                     SSNEATIEQKNSNSSISRFLHSASGIIARKMASKQVGIFSRQLATLLSAGMPLLRAIA
                     DIMDQTDHQYFKIVIADVKENLENGAALSNCLAKHRGVFSNMYINMVRVGENLGSLDE
                     VIERLADIEEKNATLKSKVQSALWYPGFMIFFSTCIVIFLLVVIVPSLAEMFEDMGHD
                     LPLPTQIIMGISSFLSAFLPLVLLSIGAGIFFLYRYVHTEKGRHKFDELKMNIPIFKI
                     LYNKMIVYRFTQNMGIMLSNNVDILKSFEIVEKIVGNSIIEKKINEAAFKIREGTPVS
                     KALAAADFLPKMVLGMINAGEASDKLDTMLVKIGEVYETEVDLTITSLTNMIEPLIIV
                     FMGIAVGGIVLSVILPIFQMNLIVQ"
     gene            complement(6466..8172)
                     /gene="gspE"
                     /locus_tag="P5123_03655"
     CDS             complement(6466..8172)
                     /gene="gspE"
                     /locus_tag="P5123_03655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020675249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015627 - type II protein secretion
                     system complex [Evidence IEA];
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system ATPase GspE"
                     /protein_id="HRX15387.1"
                     /translation="MIRSKKKIGEILVANSQISEQELKSALEVQITQNIRLGEILLKR
                     GVITDEALYSALSEQYGIPYDAKPEFHDKQGILEKVPIANLKRNKIAPYYVKKKQLNV
                     ATSDPLNFQPIDDMATLFPEYTVSPVVCRPEEIERIIINHFGAQSDENTSEMFNELKD
                     SDFDMLGGGAFSETEDILDMANEAPIIRFVNKAITEAVQERASDIHIEPYEKTLIVRY
                     RIDGILYKMHEPPKKLQSAIISRIKIMSNLNIAENRLPQDGRIQLKIGKKDIDIRVSI
                     FPTHYGERVVMRLLNKSDMSFDLETIGFSSDMLQKFKKTLDKPHGVILVTGPTGSGKS
                     TTLYSALTRLNNIDVNILTVEDPIEYQLQGVGQMQVNSKIGLTFASGLRSILRQDPDI
                     IMVGEIRDIETAEIAIQSALTGHRVFSTIHTNDAASAVTRLVDMGIEPFLISSSVNAF
                     LAQRLIRRICPECKTAYKPTAKELSELKIKVSSLKNGKLFHGKGCDKCLGTGYSGRTG
                     IYELLVVSNAIKKLIVSNADAGTIKEQAVKEGMQTLYLDGLSKVIDGTTTIEEVLRVS
                     NT"
     gene            complement(8173..9966)
                     /locus_tag="P5123_03660"
     CDS             complement(8173..9966)
                     /locus_tag="P5123_03660"
                     /inference="COORDINATES: protein motif:HMM:NF012485.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="secretin N-terminal domain-containing protein"
                     /protein_id="HRX15388.1"
                     /translation="MKINKKASLILIMICIMTLSLFISYEGNPPRLGIRNLYAQRKAT
                     FALNINDQDINEFLKMMSSLIGKNIIADNKVRGKITVISAKRIPVSEAYNIMKSILQL
                     QGFAVIESENLIKIVTIDDAISSNTTLLVDEDLNVSTREGDTITYIYNVKEGDAYKIR
                     NILNTLKSRTTKVLSYEDLNYILLSGMASEIEGLIKVAEALDKAPASSEEEGDESISK
                     GNIHVVHLQYANAKELAEVLSRVPFSETAKINTEPRTSPEPPSKTARSSRAAAAATRQ
                     TAPSSQQPKFSIIANNDTNSLIITATTEEFRQIKAIISQLDIVREQVLIEALVIEVSA
                     ENGWGFGIDWMLGAPVGQRGSDTGGLIGGSQIFNSNSANFTDKKIAGKDVVLPLNQGF
                     QLGFLSDRAQLGFMLLNAQATDKNFSILSTPQIMTVDNQEAEINVGDQFPVPSNNIVN
                     SDGTSSFSYDYKDAGLKLKITPHITKGGKVTLQLYQEVNSVKSAGTTLSNTYIPPDIS
                     KRDIKTIVSVDDGKTVVVGGLINNNKSESETKVPILGDIPVLGWLFKYKEVKYTKTNL
                     LVFITPHIVTDADKLHRITQEKKEEQKLLNR"
     gene            complement(9985..10824)
                     /locus_tag="P5123_03665"
     CDS             complement(9985..10824)
                     /locus_tag="P5123_03665"
                     /inference="COORDINATES: protein motif:HMM:NF022800.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system protein N"
                     /protein_id="HRX15389.1"
                     /translation="MNNQLKFHAATLLSVTLLAGLTSFCINMIIGNIIFAPQIDTKKA
                     VSRTRIEQSVTRSQSSQSILESTLFKLAEISDADGEETADSPAPVSDLSLLGTITGPA
                     SIARALISKRGEKNSEIFRIGQNVYGYTLTWIETTKVYLKKNDQKMILDMYPPKNAFK
                     PASSSSSSSGSSMTTTLSRSELKQNLKGSMDTMLKGLVVSPYRKDGQIVGYRLKRVSS
                     QNMLYKYGMRSGDILQKINGHQITSTQKLFELWQTIPEESKVIIEINRRGNDETLEFI
                     IND"
     gene            11402..12100
                     /locus_tag="P5123_03670"
     CDS             11402..12100
                     /locus_tag="P5123_03670"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRX15390.1"
                     /translation="MYVIYIALVCVVAGIIFLVKSTQTEKVALSDKKNGKFTASQYRG
                     GASPSSSSPLNTSGKSVIRTNEYVYSVEDSAQMANRGMESGYQISSEPGLAELNDSVE
                     YSSNADIGKESDKQYLTPGSVKANEIETVLYEDPDCAINFTLNRDLADPSVFTNLRRK
                     GSGALSLGSDSLSLRFDKTLYRFDFNRIKDIRGDKNGALIYPDNIAYPLLLIAPDCDD
                     FSERLLSAYNQFLK"
     gene            12216..13169
                     /locus_tag="P5123_03675"
     CDS             12216..13169
                     /locus_tag="P5123_03675"
                     /inference="COORDINATES: protein motif:HMM:NF025910.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HRX15391.1"
                     /translation="MYYRYKKNKGPGVLFKVLFVALFFGVIFYFTNRYQEYLFFWRYT
                     VNQISSGINSASLVEDEQRRIEILRESYKKSLDYAEANPLSREAFFTLANATASLAEA
                     ESSIKFSSYILNDNSSVFDRESKDLLLESMKALKKGIALDERQVVADSILVLASKLDF
                     YTGYSEMEKIFSYITLVSDPASLSDVEDRRFYGLMLVLKGDVDAGVAFLRNHGQIASF
                     SDRLFFSGALKKAGQYTDAIVEYKELLSEQPSKEHEVYILYNLGEIYYEQSLYNEALE
                     ELLKLQLVKPDDAKARNLIGQIYLKLGDKKSAEAYLGGFGG"
     gene            13643..14668
                     /locus_tag="P5123_03680"
     CDS             13643..14668
                     /locus_tag="P5123_03680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010574148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0000902 - cell morphogenesis [Evidence
                     IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein"
                     /protein_id="HRX15392.1"
                     /translation="MLFNSFYGMFSNDMGIDLGTANTLVHVKGQGIVLSEPSVVAQQT
                     STGKVLAVGHEAKRMLGRTPGDIVAIRPMKDGVIADFETVEKMIRYFITKVHKRKTLV
                     RPRVVIGVPSGITEVEKRAVRESAEQAGAREIFLIEEALAAAIGANIPIHEPAGHMIV
                     DIGGGTTEIAVISLGGMVIADSVRIAGDEFDEAIIKYMRNQYNLVVGERMAEEVKFRL
                     GNIFPDKKVESMDLKGRDAISGLPRTLEIDSSEIRKALKEPADQILDGIKHALERTPP
                     ELAADIVERGIVLTGGGALLKGLDKYISKDTGVPVIRAENPLTCVVLGAGKFLEELKN
                     LYRSNLK"
     gene            14808..15668
                     /gene="mreC"
                     /locus_tag="P5123_03685"
     CDS             14808..15668
                     /gene="mreC"
                     /locus_tag="P5123_03685"
                     /inference="COORDINATES: protein motif:HMM:TIGR00219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreC"
                     /protein_id="HRX15393.1"
                     /translation="MEFFIRHKAIVTLISSFIFCTVSLSVQSSTFTLSLEGLGSLFLM
                     PFQKSYNFVQKGAQVMWAGFTELDDLREELNQAHVKLQEYEAATEDLTEIRKENERLR
                     LLLGFNEKIVYKSIPAQIISKDPDNWFRTIVINKGSDDGIRPNMPVVAFTGEKKAVVG
                     KIIEVRGSLSRVQPLISPTMKVGVRLQSSRYPGLMEGYSLNSGLCIVDYINKSAFLQK
                     GDHVITSGQGGIFPPGLLVGTIEDSITMDSSAFKQALVRPLIDYNHVEEVFVILKIPD
                     LDLIELIERH"
     gene            15671..16174
                     /gene="mreD"
                     /locus_tag="P5123_03690"
     CDS             15671..16174
                     /gene="mreD"
                     /locus_tag="P5123_03690"
                     /inference="COORDINATES: protein motif:HMM:NF016019.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreD"
                     /protein_id="HRX15394.1"
                     /translation="MNVSYIFTAIILIVSLIMQGHSSFDVLRIGGVKPDMTFIAIVYY
                     SYSFGSFYGEATALFSGFFHDSMSNSPLGLLTLPKVIVAFAIGLFGRAVMKSNVLTII
                     PLVFGASVLKGVITLLLCMIFHEASVSDIAYIILPESFYNALLAIPLFLVFDKIYENE
                     LGREGSY"
     gene            16174..>17879
                     /gene="mrdA"
                     /locus_tag="P5123_03695"
     CDS             16174..>17879
                     /gene="mrdA"
                     /locus_tag="P5123_03695"
                     /EC_number="3.4.16.4"
                     /inference="COORDINATES: protein motif:HMM:TIGR03423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein 2"
                     /protein_id="HRX15395.1"
                     /translation="MTHSSFRTKMVEAFRIRVLFCYAVVLFTLVVIVFQFINLQLVQG
                     ADYELKARTNMESFVPIVASRGEIFDRTFSTTNTNNRVIVSNRPSFNISTVPAKFNSK
                     KEMQQALEKLSEIVDVDVNAVMKDYNASNPWQRVLVLEDVSFEKIVKIASHKQIYRNI
                     DWEVAPVRVYNYGSMFSHIVGYIGSISKKEFLELRDQGYQYYQKLGKSGVERQYDREL
                     RGVDGSVKRIVDVKNRTEGEEIGFQPVSGNNLVLTMDLEIQKAAYEAFEGFVGSCVVI
                     KPATGEIVALVSRPGYDPNAIISRDNSDIIRKLYNDKERPFLNRVIQSKYPPASTFKL
                     VTAIAALEEEKWEAERIVNCPGYFVLKGLRDTVIYDYAVFGNINLYEAIGKSASVYFY
                     TMGHKIGPTTIMNYANHFGLSERSGIDLPGEVSGFVPSQKWKRKAFGEGWYDGDTVNL
                     SIGQGFLAVTPISMANLVCAIVNNGIIYKPHVVKEIRSPDNNQVLKSIEPEKIREIPL
                     SPSTLNVIKTGMRLSVKSGTSRRLAYLPVPFAGKTGTAQTRSVRDSKYTQHGWFVGYG
                     PHG"
BASE COUNT         4880 a         3352 c         3799 g         5848 t
ORIGIN      
        1 ccccttttta gctgtgtttt gcttaagctt gatttcaatt gagaaacgtg aaattgaacc
       61 agtcgaagtt gtacggtcaa tggccgattc gaaaagagat acagatttga gtttagtcat
      121 aaagtcggat aatgtcgagc tggtttcaac tgttcctgac attttaatat ccttatctga
      181 tataacaagg ctttgtattt cgatgtcgct ttcttcaggc atttgagccg aaatagctga
      241 tattagctca attgtactgt tctcttctgg tatcaaattg cgtatctgat taagtttatc
      301 caactctttc tttaaaagct tgtttgccga catgataggg tcatcatccg tgggttggct
      361 tttaaaaagg gtagtatact gtttttggag cagatctctt gttaatgctt tttcacgaga
      421 tgtgaaaaaa aggttcaata ttaatacaat aatgagcatt attgccgaca gtgaagtaaa
      481 gaatagagcc ggatacagtt tctctatctg gatcgatccg aaagaactat ttttatgatt
      541 ttcacaaaaa ttaatataat ctgttttgtt tcggtaaagt tcgatgagtc cgtaacagga
      601 actgaatctt gcctgagcaa tctgattagg taactgcaca tttggaagcg aaccaaccgg
      661 aatttccatg gcattagcaa aaaaatcgga aattctaggc aacagggagg ctcctccaga
      721 aagataaatc cgttcgaaat cgacagagtg atttgctgct gtaaaagcct gaattgtaag
      781 ccggatttga tcaattatgg attcgaattc aagtatgctg aaatcatata tctcttgcag
      841 ctgttttttt gtaattttta gtgatttgta aaaattggag ctgtagttgt tttcaatatc
      901 agagagatcc agatgaagat tctcgaattt gccaaatgct tcataatagt ttatcttgag
      961 tatagatgcc atttttgaaa taatagatga acatccgcca ggtatcaggc gagtggcagc
     1021 aatttttttc ctatgtacca gattaacagt tgtttttgag tgaccgatat ctatctgcaa
     1081 aaaagagtca aactgtaaag tttctggtaa tagagattca aaaagactgt ttgattctat
     1141 tcctatgtaa tgcggtgtga tgcctgtagt accgattaca actttaaact gatttaggtg
     1201 ctccttttga atagcggcag caattatctt accctcttca gtgccttcgt tagcgagctg
     1261 gaaagatata tcaatatcat caagatcgta aggaagagtc tcttcggctt ccattggcag
     1321 tatttctgag atctttgatt tctcggaaaa cgggaaagaa aagttcctta aaataacaga
     1381 ttttaaagga atatttgtaa ttagcaaata atcattatca gtaaattcac tcatcaggtc
     1441 tgtaagagcc tcttctatct cggcctgttg atcggcggat tcgggattga tcatcctgtg
     1501 agaaaaatga ataatctcgt ttgatttaaa gccccttttg gcagatagtg ccttaataga
     1561 agatgttcct atatctatag ccaataattt ttcaaacaaa atgcacctca agtatcggtt
     1621 ttatattaaa tggttgtttc caatttgtaa acactttgat ctaacgtcgt ttcccattca
     1681 gaagtactca tgaccggccc gttgtaaagt aatttttaat ctagttttaa tgcaaagcat
     1741 aatagctgta gtacttgaga gacctgtcat aaattgctgt tattgttact ttatgacgat
     1801 tcacataggc atgaaccgtt attctgaaaa gattagagtt aactgtaagg cagttacgtc
     1861 gtatcagatc atcacgaata aattccttaa catcatcaac ttttttaaaa ggattaatgc
     1921 gtcttcgtaa aattatctgc tttgcgtcgt tatcggtcat atcgggtgct atcgaacgaa
     1981 gaacacggta cggtgcggta tttatattta tcttgttaac ctgactgtta aataacttgg
     2041 ggtcaccgtt gacccttaga tacttgtcaa gtctacgtga actttccggg cctattggga
     2101 tatcctcttc ctcggattca cccgactctt cctcctctgt cgtctcttca gaatcatcag
     2161 agaccgactc cataatggat agtacatcaa gtgacattat ttcatcattt gattctacaa
     2221 gcatgccttc ttctttctct tgagccatat tgccaccgct aaaaccatag tatatagccg
     2281 gtgaaaaata gcgtaccagc aacagtctgt ctatactttc aagaggagcg tttgcggccg
     2341 aatagggtgt ctgtagggta gagtagtgat cataggtttc ggctccattg cctgtacgac
     2401 tgttgtcgcg gtctacccaa tcagcaacag catcagcata gtccattgga aaacccatat
     2461 tcataaaaaa acggttaatt atgccaaaga aggttgtttg tggaacgtat tctgtcgcga
     2521 tagcacttag gtttattttg gattgctcat cttcaataat tattttaaca gtagcgttcg
     2581 ccagttcgaa ttccgggaaa tcaaatgccc agatatcacc aaaggtatcg atattcttat
     2641 ctttgttttt tactggtatc atggggggag tcacaccttt ttggtcaata tcaagaataa
     2701 tatgtgcgat attcagggct gcctgtgctg ctgcttcggc ctgagcctca tttttgaatt
     2761 taatcatgta gtttatatta ttttgggcac tcacaagaaa atcggttgat acggatatca
     2821 gcagcgtact gacgatcaga acaatgatta gtacaaaacc cctgttcttt tcattgctgc
     2881 cgaacaagcc gtgcttttcc agtttagcgg ctctttgacg atacatctgt ctgatctgac
     2941 taatatgttt ttttatattt atcattctat tggtacctgt taagcaatga tgtaaacgta
     3001 aagatctcat cctgtcccct atagtttcgc agggtaagag tcacccttat tccgaaagga
     3061 aagcggttgc ggtctctgct gtcccaattt tgagaccagt cattaatctt gtcttcggtg
     3121 tactctattc tcatatcgat tacgttttca agcagtatcg agtcaatacc ctgtggctta
     3181 ttttcatcat ctttgttgta ttcgatggat tcttttcgca taagaaaaaa catatcagga
     3241 taagatggat caggtttaag aaagtatgat atttctttaa tgtcagaatt aaaagcaggt
     3301 tcggaatagc tgcctgcaac atatatctcg gatttaacaa cggttaccat gttgattttt
     3361 cccatatagg gtgggtaggg attattttca ccgactaaaa aaagttcttt gttatatctg
     3421 tcttgcaagg tgcagcttaa gtctctgtcc attcgacgta ttgccatatt tgtattctca
     3481 taaaaatcgg caattccaga catatcatga attgcgaaca taatggtgcg gtttgcgcca
     3541 tatatcatca tcatgactat actggatata gtcaccgcaa caagcaattc aagtaaggtg
     3601 aatccttcat ttgatttaag ccgtttaata aattttttca taatggataa atgtattcga
     3661 tagagtagga ccgttcagca ttattttcca tccagaaaac agttactact gcgatattag
     3721 ctggtatggg tcccagaagt tcatgttcaa atttttcgat acttatctca taggtaaaat
     3781 cgggatattc cggtacttcg gatcctgcaa taacattgtt ttgcattttt tgaatcttaa
     3841 gttcatttag ttttgatctg gcgataacaa tagccttggt tagattttta ttttgagaga
     3901 ttgccataga gcccgtggat attcccttgt aaagaggaag aactaccagg ctgagaattg
     3961 ttacggcaat aagtatctct atcagggcaa aaccgagttt attaaaaacc ggatttccac
     4021 attgattcga agtcaatatg cccctccttt gtataaggat atttcatcag tttagaaata
     4081 attatagaat aacgttcggt atcgttgaga gtaacatgga ttatagcgtt tttagaaaag
     4141 ccgtcaggat aaaagggaat aagagccgta ccggatgaga tgtcgcttcc atcatcaagc
     4201 agaacacggt taatcataaa cccagaagaa aactgtctcg gtttaagcat cttattttgc
     4261 gagtcaatca acagaccttc gggcgacaca ttaaccacgg atattccgtt tgatctttcg
     4321 aggatctcct tgttttggtc gtcaaactcg gttacggaag taaaatagag gtgtaccgca
     4381 agaaaattga ttctgttatt gataaaagca tcatcaaagg ttttcacgac catggaagag
     4441 acaagtgaca gatttttctg tgaattgtca aaaatattgg ataccctcgg tacaatcact
     4501 attgaaacga tagagagcag ggcgataaca atcaggatct ctatgagaga gaaaccctga
     4561 ttgctctgct tccggttatt cccagctggt aatatccgca tttactcctt ctccgccttc
     4621 ctgattatcc gcgccaagac agatgatctc gtaaggtcgt ccctcttctc cgggagatac
     4681 gtatataaaa tcatttttcc atggatcttt gggtacagag cttgactgga tatagcctcc
     4741 agtcttgtag ttgttgggta ccggctccga tgttggagct tcgattaatg ccgcaagccc
     4801 ttgatcagtt gtagggtagg ttccattatc catcatgtag aggttcagcg atagttcgag
     4861 agattttatc atctcattag ccttggatac cttagctttg tccggcatat ccataaactg
     4921 gggaactatg gcgattgcaa gaatactcat tattgtaacc gcaatcatta tttcaagaag
     4981 cgagaaaccg tctcgagcct ttagcttttt gattactgtt gatatgtgtt taaacaattt
     5041 tttcagtata agcatgataa tctccttttt ttgggaactt tgaaaaatta cttttttcag
     5101 aatggattcg actttttgta aaatataaaa aactctacca actaagaaac atgttatagc
     5161 gtttttgttc catttttttt aaaaaatttt tatccgcaca aaaataatat gcgatttact
     5221 gcacaattag attcatctgg aaaattggca aaataacgga taatactata ccgccaaccg
     5281 ctattcccat aaatactata ataagaggtt ctatcatatt agtcaaactt gttattgtca
     5341 ggtcaacctc ggtttcgtaa acctctccaa ttttaaccag catcgtatca agtttgtcac
     5401 tggcttcccc cgcgttaatc attcccaaaa ccatttttgg aagaaaatct gcagctgcaa
     5461 gggcttttga aactggagtt ccttctcgta ttttgaaggc tgcctcattg atttttttct
     5521 caataatgga gttacccact attttctcta ctatttcgaa tgattttaaa atatcaacat
     5581 tgttgcttaa cattataccc atgttctgag taaacctata aacgatcatc ttgttgtaca
     5641 aaattttaaa gataggaata ttcatcttca gttcatcaaa tttatgtctc cctttttctg
     5701 tgtgcacata gcggtacaga aaaaaaatac ccgcaccaat tgataagagt accagaggaa
     5761 gaaaggccga caaaaacgag cttattccca taatgatctg tgtaggcaga ggaaggtcgt
     5821 gtcccatatc ctcgaacatc tctgcaagag aaggaacgat aactaccagg agaaaaatca
     5881 ctatacatgt ggaaaaaaag atcataaatc cgggatacca gagtgccgat tggaccttac
     5941 tttttagggt agcatttttc tcttcaatat ctgccagacg ctctattacc tcatcaaggg
     6001 atcccaggtt ttcacctaca cgaaccatat tgatatacat gttcgagaag acacccctgt
     6061 gtttcgcgag acagttagac agcgctgctc cgttttcgag attctctttt acatcggcaa
     6121 ttactatctt aaaatattgg tgatcggtct gatccataat gtctgctatt gctcgcaaaa
     6181 gtggcatacc cgctgataga agtgttgcaa gttggcgaga aaaaatacca acctgctttg
     6241 atgccatttt tcgcgctatg atgccacttg cggaatgaag aaatctggaa atagatgaat
     6301 tagagttttt ttgttcaatt gtggcttcgt ttgatgattt agggccgatt tttgatacat
     6361 aaagaccctc ttgtttaagc attagcttgg cttttgtttc acttgtcgcg tcaacgatgc
     6421 ctgagagtct ttctcccctg cgattaaggg cttcgtactg gtatatcatg tgttactaac
     6481 ccttaagacc tcttcaatag tagttgttcc gtcaataacc tttgacaacc cgtctaggta
     6541 cagggtttgc atgccctctt ttacagcctg ttcttttatt gtcccggcgt cagcattaga
     6601 tacaataagc tttttaatgg cattggatac tactaacagc tcgtatatac cggtacgtcc
     6661 gctgtagccc gtaccaaggc atttatcaca acctttgcca tgaaaaagtt tgccattttt
     6721 aaggcttgaa actttgatct taagctctga gagttctttt gctgtaggct tataagctgt
     6781 tttgcattcg gggcatatac gtctgataag acgctgtgca agaaaagcgt taactgatga
     6841 tgatatcaga aatggctcga tgcccatatc tacgagtcgt gttacggccg atgcagcgtc
     6901 gtttgtgtgt atggtcgaaa atactctgtg gcctgtaaga gccgactgta tggcaatttc
     6961 ggcagtttct atgtctcgaa tctcacccac cattatgata tccggatctt gacgcagtat
     7021 ggagcgaagc cctgatgcaa aggttaaacc gattttagag ttcacctgca tttggcctac
     7081 accttgaagc tgatactcta ttggatcttc tactgtaaga atgttaacgt ctatattgtt
     7141 aagtctggtt aatgcgctat atagcgttgt ggatttacca cttcctgtcg ggccggttac
     7201 caagattact ccatgaggct tatcgagtgt tttcttgaat ttttgcaaca tatcactgga
     7261 aaatccgatt gtctcaaggt caaagctcat atcagattta ttaagaaggc gcattacaac
     7321 acgctcacca taatgagtgg ggaaaatcga aacacggata tcaatatcct tcttgcctat
     7381 cttaagctga attcgaccat cctggggaag acgattttcg gcgatgttaa ggttagacat
     7441 tatttttata cgcgaaatta tcgcactctg cagctttttt ggaggttcat gcatcttgta
     7501 cagaatgccg tcaatgcgat agcggacaat gagtgtcttc tcgtaaggct ctatatgtat
     7561 atcactggct cgttcttgta cagcttcggt gatcgccttg ttaacaaaac gtattattgg
     7621 cgcctcattg gccatatcga ggatgtcttc tgtttcgcta aaggcaccac cgccaagcat
     7681 atcgaaatca gaatccttga gttcgttaaa catctcggag gtgttttcgt ctgattgtgc
     7741 tccaaagtgg ttgattatta ttcgttcgat ctcttcagga cgacagacta ccggagaaac
     7801 agtatattca gggaagaggg tagccatatc atcaattggt tgaaagttta ggggatccga
     7861 ggtggcaaca ttcaactgtt ttttcttcac ataatagggg gctattttgt tacgcttcag
     7921 atttgctatg ggaacctttt caagaattcc ctgtttatca tgaaactctg gtttcgcatc
     7981 atagggaatt ccgtactgtt ccgaaagggc actgtataga gcttcatcag taattacgcc
     8041 tctttttaac agaatctctc cgaggcgtat attctgtgtg atctgaacct caagagctga
     8101 ttttagctcc tgttctgaaa tctggctgtt ggcaaccagt atttcgccaa tttttttctt
     8161 agatcttatc atttatctgt ttaacagttt ctgttcttct ttcttttctt gggttattcg
     8221 atgcagcttg tccgcatccg ttacaatatg aggcgtaata aacacaagta aattggtctt
     8281 tgtgtattta acttccttat acttaaatag ccatccaagt accggtatgt ctccgaggat
     8341 aggcactttc gtttcggatt cagatttgtt gttatttata agtccaccga caacaactgt
     8401 tttgccgtcg tctaccgaga ctattgtctt tatatcccgt ttgcttatat ctggtggtat
     8461 atatgtgttg ctcagagttg taccggccga cttgaccgaa tttacttcct ggtacagctg
     8521 caatgttacc tttccaccct tggtaatatg tggtgtaatc ttcagtttca atccggcatc
     8581 cttatagtcg tagctgaagc tcgaagtacc gtcagagttc acgatattat ttgagggaac
     8641 gggaaactgg tcgccgacat taatctctgc ttcctgatta tccactgtca ttatttgcgg
     8701 tgtagaaagt atgctgaagt tcttgtcggt ggcctgcgcg ttaagcagca taaagcccag
     8761 ctgtgccctg tcgctcaaaa agcccagctg aaatccctgg ttaaggggaa gcacgacatc
     8821 cttacccgcg attttcttgt cggtaaagtt cgccgagtta gagttgaata tctgagaacc
     8881 gccaataagc ccaccagtgt cggatccgcg ctggccaacg ggggcgccaa gcatccagtc
     8941 gataccgaaa ccccatccgt tttcggccga tacctctatc acgagtgctt cgattagaac
     9001 ttgttctcgt acaatatcaa gctgtgatat aatggccttt atctgtcgaa attcctcggt
     9061 tgttgcagtt ataatcaatg aatttgtgtc attattggca ataattgaaa acttaggttg
     9121 ctgcgaagag ggagctgtct gtcttgtagc tgcagccgct gctcttgaag atcttgccgt
     9181 tttggaaggc ggctccggag atgttcgtgg ttcggtgttt attttggcgg tttccgaaaa
     9241 aggtactcta gaaagaacct ccgcaagctc tttcgcgttg gcatattgaa gatgaacaac
     9301 gtgaatattt cccttgctga tactctcgtc gccctcctcc tcggaagatg ctggtgcttt
     9361 gtcaagtgcc tctgcaacct tgattagtcc ttcaatttca ctggccatgc ccgagagcaa
     9421 tatatagtta agatcctcat aagatagaac cttggtagtt ctagatttaa gagtgttaag
     9481 aatattccgt atcttatagg cgtcaccctc tttgacattg taaatgtaag ttattgtgtc
     9541 gccttcacga gtagagacgt taagatcctc gtcaacaaga agggttgtgt ttgaagatat
     9601 cgcgtcatca atggtaacaa tttttataag attttcgctc tcgataaccg caaagccctg
     9661 aagttgcaga attgacttca taatattgta ggcttctgaa accggaattc ttttagcaga
     9721 gattacagtg attttgcccc ggaccttgtt gtcggctatt atgtttttgc cgatcagaga
     9781 gctcatcatt tttagaaatt cgtttatatc ctgatcgtta atattcagcg cgaaagtagc
     9841 tttacgttga gcatataagt ttcttatacc aaggcgtggg ggattaccct cgtaagatat
     9901 aaaaagcgag agtgtcatga tgcaaatcat aatcagtata agcgatgctt ttttgttaat
     9961 tttcataatg tcctgtttgt ccggttaatc atttattata aattcaagcg tttcgtcgtt
    10021 accacgacga ttaatttcaa taattacttt actctcttca ggtatggtct gccatagttc
    10081 gaaaagtttt tgagtgctcg taatctggtg tccgttgatc ttctgtaaaa tatcaccgct
    10141 tcgcatgccg tatttgtaaa gcatattttg gctactcact ctttttagac ggtaacctac
    10201 tatctgccca tctttacggt atggagagac aactagacct tttagcatgg tgtccatact
    10261 accttttaag ttctgtttga gttcggaacg gcttagtgtc gttgtcatgc ttgaaccgga
    10321 tgatgaactg cttgaagaag cgggcttaaa agcatttttc gggggataca tgtccagtat
    10381 catcttttga tcattttttt taagataaac cttggtagtt tcgatccagg ttagggtgta
    10441 accgtagaca ttctgtccga ttctgaatat ttcagagttt ttctcgcctc gtttgcttat
    10501 aagggctcga gcgattgaag caggccccgt tatcgtacca agaagtgaca aatcagagac
    10561 gggtgccggt gaatctgcgg tctcttcgcc atctgcgtcg cttatttcgg caagtttaaa
    10621 aagagtgctt tcaagtatgg attggctaga ttgtgatcga gtaacagact gctcgattct
    10681 tgttcgcgaa actgcttttt ttgtatcaat ttgcggcgca aatatgatat tgccgataat
    10741 catgtttatg caaaaagagg tcaaccctgc caatagtgta acagaaagca gtgttgccgc
    10801 gtgaaatttt agctggttat tcatataata atctctaatg tttaacgttt cgcaattttg
    10861 tatgaaaaaa ctgattacat agttgctatt tgtactcatg aactacgcaa gtataatcta
    10921 aatttgtgac tcttttggta atattttaag tgctcgctta gcgcaacctt attttttttt
    10981 ctgagactac tctgtcaata caatttcatt tgatttcggc tttcagcgtt tgccaaatag
    11041 tattttgcta aggtattttg taagaattat gctaatagct tgttatcgat tatggtttat
    11101 cgccctgaaa tagaagaatc atctgtaacc gtcggctttt tgaaacacta acattctgtc
    11161 cggattatat cttgacagaa gaaaccgtgt aattagtatc agatgatatt gttggcgtct
    11221 tgttacaggt gataagaaaa gaacacgatt cttgattgac ttttggttaa cgtgttgtgc
    11281 taaaatgaac cgcacttgat agtttttgat taattttata taaatgaaaa acagtaatat
    11341 ttacaagctc tatagttgtg ttttgctgta acagggtgga aaattttctg gaggaatggc
    11401 aatgtatgtt atttacatag ctcttgtttg tgttgtggcc ggtataatct ttcttgttaa
    11461 atctactcaa accgaaaaag ttgcgttatc tgataaaaaa aatggtaaat tcacggcttc
    11521 gcaatacaga ggtggggctt ctccgtcgtc atcatcccct ttaaacacat ccggcaaaag
    11581 tgttattcgc acaaacgagt atgtctactc tgtagaagat tctgctcaaa tggccaatag
    11641 gggcatggaa tctggatatc aaatctcttc ggaaccgggg ctggctgaat taaatgattc
    11701 tgtcgagtat tcttccaatg ccgatatagg taaagagtct gataaacagt atcttactcc
    11761 gggttcagtg aaggccaacg aaattgaaac agtcttgtat gaagatccgg actgtgcgat
    11821 caattttacg ttaaacaggg atttggcgga tccttctgtt tttacaaatc ttcgcagaaa
    11881 aggctccgga gcgttgagtc tgggctctga ttccctgagt cttcgttttg acaagactct
    11941 ctatcggttt gattttaacc gcataaagga tattcgtggt gacaaaaacg gagcgctgat
    12001 ttaccctgat aatattgcgt atccgctttt gctgatagct cctgattgtg atgacttctc
    12061 tgaaaggctg ttgtcggcat ataatcaatt tctgaaatag ggctgttctg tctttatcaa
    12121 tgaaagtcta tccgggttgc agacttttgg tgtattaaat aaatctgtcg tccgctggtg
    12181 ttgtctaaaa tattgaattg ctggggtttt tttggatgta ttatcgatat aaaaagaata
    12241 aagggcctgg tgtgctgttc aaggttttgt ttgtagcact gttttttggg gtgatcttct
    12301 attttacgaa tcgttatcaa gagtatctct ttttctggag atatacagta aatcagattt
    12361 cgtctggaat aaactctgca tcgcttgtgg aagatgaaca gaggcgtatc gagatattga
    12421 gagagtctta taagaaaagt cttgattacg ctgaagctaa cccgctttcc agggaagcgt
    12481 tttttactct tgcgaatgct acggcaagtc ttgctgaggc agaatcttct ataaaatttt
    12541 cgtcttatat attaaatgat aacagttcgg tatttgatcg agagtccaag gatcttcttt
    12601 tggaatcgat gaaagctttg aaaaaaggga tagctcttga tgaacgtcag gtggttgccg
    12661 atagtatcct ggtgcttgct tccaaattgg atttttacac cggatattct gaaatggaga
    12721 agattttttc gtatattact ctggtttctg atcccgcttc actgtccgac gttgaggatc
    12781 gccgttttta cgggcttatg cttgttctta agggagatgt ggacgcaggt gttgcatttt
    12841 taaggaatca tggacaaata gcatcttttt ctgacagatt gttttttagc ggcgcgctaa
    12901 aaaaagcagg tcagtataca gacgcaatcg tggagtataa ggagcttctt tccgagcagc
    12961 cgtcaaaaga gcatgaggtc tatatccttt ataatctggg cgagatatac tatgagcaat
    13021 cactttataa cgaagccctt gaggagcttc ttaagcttca gctcgtaaaa ccggatgacg
    13081 ccaaggcgcg gaatttgata gggcagatat acttgaaact tggcgataag aaatccgcag
    13141 aggcgtatct gggtggtttc gggggatgat aaagagagtg tagacctctt ttaaaaacct
    13201 tgacaaaaag cagaataaaa aaatagtatc acttctgagc gttcgtttgt ttgctggttt
    13261 ttgttttcta tgataatcat tttactgtcc ggtttcctcg agctgggaac agagtgagga
    13321 tttttaaaac atttaataat tacatgcgtc aggctcggct gttctttttg gcggtcggtt
    13381 tttatagatt cacagagcac tgagattttt ctgttacgca aagcctggta tttctgtttt
    13441 ctaagtcagg attttgggtc tcaaatacaa tagacttaaa aattaaggtt tttgcagctt
    13501 gtcttgatcg gttcattttt atgggtaatt cgacaatatc actatgataa atgataatgt
    13561 atgaagatat cttgatagac aagaagtttg taagctggtt ttgtcatgtt agctgatgtg
    13621 attgattcta aagaggtgtg aaatgctttt taattcattt tatggtatgt tttctaatga
    13681 tatgggtatc gatctgggta ccgcaaatac tcttgttcac gttaaagggc agggtattgt
    13741 gctgagcgag ccgtctgttg ttgctcagca aaccagtaca ggcaaggtgc ttgctgtagg
    13801 ccatgaggcc aagagaatgc ttggacgtac tccaggtgat attgttgcca ttcgtcctat
    13861 gaaggacgga gttatcgcag attttgaaac tgtagaaaaa atgattcgct attttattac
    13921 caaggtgcat aagcgaaaaa ccttggttcg cccgcgagtt gttataggtg ttccttcggg
    13981 gattaccgag gtagagaaaa gagccgttcg tgaatctgcc gaacaggccg gagcacgtga
    14041 aatatttctt atagaagagg ctcttgcggc tgctattgga gcaaatattc caattcacga
    14101 gccagctggc catatgatag tcgatattgg tggaggaaca accgagatcg ccgttatttc
    14161 tcttgggggt atggttatcg ctgattccgt tcgtattgct ggtgatgagt ttgatgaagc
    14221 aataatcaaa tatatgagaa atcagtacaa tcttgttgtt ggagagcgga tggctgaaga
    14281 ggttaaattc cgtttgggta atatttttcc ggataaaaag gttgagtcaa tggatcttaa
    14341 aggacgtgat gctatatccg gacttccacg tacactcgag atcgattcat cagaaattcg
    14401 taaagcctta aaagagccag ctgatcagat tctcgatggt ataaaacatg cgcttgaaag
    14461 gactccgccg gaactggcag ctgatatcgt tgagcgtggt atcgtgttaa caggtggagg
    14521 cgctcttttg aaggggcttg acaagtatat aagcaaggat accggtgttc cggtaattag
    14581 agcggaaaat ccgctaacct gtgtagttct tggagccgga aagtttcttg aagagctcaa
    14641 aaatctttac cgatctaact tgaaataaaa tttgttaata ttataaaaac cggcggctgc
    14701 tgattctttg gcaaaagccg gttatgcgcc gggtttttat cttaagcata aaatgataaa
    14761 aacctttttt ttagttaatt ctctgtatca gttaatttgg tgtgtttatg gaatttttta
    14821 tacgacataa ggctattgtt actctgataa gctcctttat cttctgtacg gtttcgcttt
    14881 ctgttcaatc ttcgacattt accctgtctc tggaagggct tggaagtctg tttcttatgc
    14941 cgtttcaaaa aagctacaac tttgttcaga aaggagctca ggtcatgtgg gccggcttta
    15001 cggagcttga cgatttgcgc gaagagctta accaggcaca tgtcaagttg caggagtatg
    15061 aagcggcgac ggaagatctc actgagattc gcaaagaaaa cgaacggtta cgtcttttac
    15121 taggttttaa tgagaagatt gtttataaat caataccggc acagatcatt tctaaagacc
    15181 ccgataactg gtttcgaacc atagttatta acaagggatc cgatgatgga attcgtccca
    15241 acatgcctgt tgttgcattt acgggggaaa aaaaggctgt agtcggaaaa atcattgaag
    15301 tgcggggttc gctttctagg gttcagccgc ttatatctcc gacaatgaag gttggagtgc
    15361 gtctacagtc tagccgttat ccagggctca tggagggcta ttctctgaat tctggtctct
    15421 gcattgttga ttatattaat aagtcagctt ttttacaaaa aggtgaccat gtaataacct
    15481 caggacaggg tggtatcttt cccccaggcc tacttgttgg tactattgaa gattcaatta
    15541 ctatggattc tagcgctttt aagcaggcgc ttgtaagacc gcttatcgat tataatcacg
    15601 tagaagaagt ttttgttata cttaaaatac cggatctgga tcttatcgaa ttaattgaaa
    15661 ggcactaaaa atgaatgttt cttatatctt tacggcaata attcttattg taagccttat
    15721 tatgcagggt cactcatcgt ttgatgtctt gcgtattggt ggcgttaagc ccgacatgac
    15781 ttttatagcc attgtctact actcgtactc ttttggatca ttttatggtg aagcaacggc
    15841 tcttttttcg ggcttttttc atgattcaat gtcaaattcg cctttgggtt tgttaactct
    15901 acctaaggtt atagtagcat tcgcgatagg cctctttggc cgtgcggtta tgaaaagcaa
    15961 tgttctaaca ataatcccac ttgtttttgg cgcttctgta ctaaaaggtg taattactct
    16021 gcttctttgt atgatatttc atgaggcttc ggtttcagat atagcttata ttattcttcc
    16081 cgagtctttc tataacgcgc ttttggccat tcctcttttc cttgtttttg acaagattta
    16141 cgagaatgaa cttggtcggg agggaagtta ctaatgactc atagctcttt tcgaaccaag
    16201 atggtggagg catttcgtat ccgtgtactc ttctgctatg cggttgtttt gttcactctg
    16261 gttgttattg tttttcagtt tattaatctt cagcttgtgc agggtgccga ttacgagcta
    16321 aaagccagaa ccaatatgga aagttttgtt cctattgttg catcgcgtgg agagattttc
    16381 gatcgcacat tctctacgac caataccaat aatagggtaa tcgtatcaaa ccggccttct
    16441 ttcaacatct ctacagtacc agctaaattc aactcgaaga aagagatgca acaggctcta
    16501 gaaaaattat cggaaatagt ggatgtcgat gtcaacgctg tgatgaaaga ttataatgcc
    16561 tctaacccgt ggcagcgagt cctcgttttg gaagatgtct ctttcgaaaa aattgtaaag
    16621 attgcttctc acaaacagat atatagaaac atagactggg aagttgctcc ggttcgggtt
    16681 tataattatg gctccatgtt ttcgcatatt gttggttata ttggaagtat ttcaaaaaaa
    16741 gaatttctgg aattacgtga tcaaggttat cagtattacc agaaactagg taaatcgggt
    16801 gttgaacgac agtacgatag agaattgcgt ggtgttgatg gaagcgtcaa acgtatagtt
    16861 gatgtaaaaa accgtacaga gggcgaagag ataggttttc aacctgtatc tggtaataat
    16921 cttgttttga ctatggatct agaaatacaa aaagctgcat acgaagcctt cgaagggttt
    16981 gttggttctt gtgttgttat aaaacccgcg acaggtgaaa ttgttgctct ggtcagtcgg
    17041 cctggttatg atcctaatgc gataatatct cgtgataata gcgatattat cagaaaatta
    17101 tataatgata aagaacgtcc atttttaaac cgcgttattc agtcaaaata tccaccggcg
    17161 tcaactttta aacttgtgac agccattgct gctcttgaag aggagaaatg ggaggcagag
    17221 agaattgtaa actgtccggg atattttgta ttaaagggtc ttagggatac tgttatctat
    17281 gattatgctg tttttggcaa tataaacctt tatgaagcca ttggaaaatc agcttctgta
    17341 tatttttata cgatgggtca caagataggc ccaacaacca ttatgaacta tgctaatcat
    17401 tttggtctta gtgaacgaag tggtattgac cttcccggtg aggtttccgg ttttgtgccg
    17461 tctcagaaat ggaagcgaaa ggctttcggt gagggatggt atgacggtga tactgtaaac
    17521 ctatctattg gtcaggggtt tttagccgtt actcctatca gtatggcaaa tcttgtatgt
    17581 gcgattgtaa acaatggaat tatctacaag cctcacgttg tcaaagaaat tcgttcacca
    17641 gataataatc aggtattaaa atcgatcgag cccgaaaaga ttagagagat tccgttgtct
    17701 ccctctactt tgaatgttat aaaaactgga atgagacttt ccgtgaaaag tggtacttct
    17761 cgtcggcttg cttatttgcc ggtacctttc gcgggtaaaa ccggaaccgc ccaaaccaga
    17821 tcggtgcgcg attcaaaata tactcagcac ggatggtttg ttggttatgg accgcatgg
//
LOCUS       DAOTGZ010000032        16855 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_3064
            NODE_4104_length_16855_cov_4.729583, whole genome shotgun sequence.
ACCESSION   DAOTGZ010000032 DAOTGZ010000000
VERSION     DAOTGZ010000032.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868088
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 16855)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 16855)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:22:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,785
            CDSs (total)                      :: 2,751
            Genes (coding)                    :: 2,732
            CDSs (with protein)               :: 2,732
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 12 of 19
            Pseudo Genes (internal stop)      :: 5 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16855
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4104_length_16855_cov_4.729583"
                     /isolate="AMR_MDS_3064"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            244..792
                     /locus_tag="P5123_03700"
     CDS             244..792
                     /locus_tag="P5123_03700"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRX15396.1"
                     /translation="MKIQKIQKILSISLILVLTVLMSCEIEDDNDENSNNGLDFRIAA
                     VNVNGIVSDTNSETYSYNDRGYLTTLLVKDGDGNTISMTSFEYSSEGLLVKKVQTSGG
                     ETVITQNEYDENRKIRSTMKDQSEIILGIINFYYDDRGRIAKKEEKDASGFIKGTADY
                     IYEEGAPKGDSEDITLRILKNR"
     gene            complement(890..1477)
                     /locus_tag="P5123_03705"
     CDS             complement(890..1477)
                     /locus_tag="P5123_03705"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRX15397.1"
                     /translation="MEFVEKIVSRRFFIILFFLVLSLFIINRLGASKNGYSIGDIGPA
                     GGIVFYDKGDNSDGWRYMEAAPKAVGRAVWGCYRYSMGGTGGKTVGTGRENTINHVEH
                     CSEKIFAAKMAHSYRGGGKDDWFLPSKDELEMIYTNLISKGLIENENHHYWSSTSYSI
                     HYAWNIATYERNKGKQYYWGKYNAFYVMPVRYFSE"
     gene            complement(1668..2450)
                     /locus_tag="P5123_03710"
     CDS             complement(1668..2450)
                     /locus_tag="P5123_03710"
                     /inference="COORDINATES: protein motif:HMM:NF012422.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HRX15398.1"
                     /translation="MFAVLLSIFSRLFLSVQYLADYGINNSGITITASILDIISLVIT
                     TLALPCLIHTYLEKKIPMFVTPISIIQIFSLPFIIFYFDGTYQSSFLYAVIVCGALFL
                     IVHMYCLILMAINLYNKTLFQRSYLVYTFILGIGYLVPGILDIFWPFFPKFIFLKEQG
                     FNFSIFIPLLISFLVIPRSYHYLIKIGSDISENISYMLKKYQFTVREKEVFYLILKGY
                     TNNQIAANLQISDSTVKKHVYSMFQKTDSENRIDLINCCRDN"
     gene            complement(2730..2954)
                     /locus_tag="P5123_03715"
     CDS             complement(2730..2954)
                     /locus_tag="P5123_03715"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRX15399.1"
                     /translation="MKTRQKKPMGFKEMLDKYISIKGLDIVVHLKDGKSIELNKNRDL
                     IEDEIVMFDKKNLETRIAIPDVSSIDLYAM"
     gene            3354..3695
                     /locus_tag="P5123_03720"
     CDS             3354..3695
                     /locus_tag="P5123_03720"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRX15400.1"
                     /translation="MGKITRTYIFFIMFFLGCSSNYVQSTNNIAKFKTEVIPYDKSLL
                     LCKITGNSMDSALYIKSYELVYNIDNVHIYINKSLKKTGISFPYFIQFIISKNINYVY
                     LGDSEIIWRRE"
     gene            3802..4536
                     /locus_tag="P5123_03725"
     CDS             3802..4536
                     /locus_tag="P5123_03725"
                     /inference="COORDINATES: protein motif:HMM:NF013980.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M48 family metallopeptidase"
                     /protein_id="HRX15401.1"
                     /translation="MNKTILKEFNIRYKADKRSRSVKIRAAEFNTLDITYPPSLSLNV
                     VNDIIVSRADSIRNAYNKVKASSMSVDSFVREKNNLLRIADSVLYFEKSSDPKLNGVT
                     KIKNGDRFFYFSKNDGLKDPDTARLAAKSIIMFCTKCAKKILPQTVKKLADKHGFSYN
                     NLSFRNQKRRWGSCSHDNNLSLNVRLMLLPYKLIEYVLIHELVHTIHKNHSSTYWDAV
                     IRILPDAKLLRKQLKKYDFTFLDRNN"
     gene            4564..5292
                     /locus_tag="P5123_03730"
     CDS             4564..5292
                     /locus_tag="P5123_03730"
                     /EC_number="2.7.1.107"
                     /inference="COORDINATES: protein motif:HMM:NF013391.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase"
                     /protein_id="HRX15402.1"
                     /translation="MNFLRFVYYFLRRYYDSVNYAIEGILHAAKTQSHVRFHLSAACF
                     LLLVCFSIGISPSEFVILTTLATIVIVAEMLNSAIEVTVDIASPEHSEKARIAKDIAA
                     GAVLVAAAAAAIIAFFIFYPYFHYFIEYGIVIAQHGVVDLIFGSTMIVLITVIMMKAY
                     VGKGHPLRGGLPSGHSALAFTWWVNIVFIYKDFYLTVIFFACAIAIAVSRVVRKIHTI
                     YEVCIGIMTGTVITWGLYLIFYKG"
     gene            complement(5289..6176)
                     /locus_tag="P5123_03735"
     CDS             complement(5289..6176)
                     /locus_tag="P5123_03735"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRX15403.1"
                     /translation="MIKSGFFHIVAILLVLQPALSQNEIVELQVSHGKSYFGIPLRWT
                     AYEGTDRYDIYRSEYKKGKYDRIATVTKKTNYTDTNCEPGVEYYYKVLPVINGRKTQF
                     SHEKSGYRLIDVSERFSLNSVLNKKKQEISFISTDDKRRLNLLESEYIGWLRIRFILL
                     ISRPYFYSNKVIVLSDFDQFTASESQNTINFLPADDDYRLTFYARRPFALLQRTGDQQ
                     LFDRLIRNSIAFCVYQGDVKIRDSKGRSKYVPQYEAIGLATHYYKYSEDWAWRTVLFS
                     SEREEIMQQMHQIEEGSKN"
     gene            6403..6768
                     /locus_tag="P5123_03740"
     CDS             6403..6768
                     /locus_tag="P5123_03740"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRX15404.1"
                     /translation="MDYSQFSQLNDQTDMDIIEFGQQTYLIGCKYDEIASSLLKRCSD
                     KMFEHEADISVCDGSNCAIFRSKSLYIDVKTGEVIVQTIDGKSAPFKMLMIEAKEMLI
                     NYLHIHVKTADIHNNFFNS"
     gene            6891..7823
                     /locus_tag="P5123_03745"
     CDS             6891..7823
                     /locus_tag="P5123_03745"
                     /EC_number="3.1.3.16"
                     /inference="COORDINATES: protein motif:HMM:NF018884.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004722 - protein serine/threonine
                     phosphatase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PP2C family protein-serine/threonine
                     phosphatase"
                     /protein_id="HRX15405.1"
                     /translation="MSDIQYKLMKNANLSIEDIIRQYSMIEAEAEEVRNNDKLTVKNL
                     FGSFYGKKLNVGIGFQRAYGSILSGDYFELYKLPDSNYLFVFADISGHGLPAYTTLIR
                     LRSAITSTLKMYEKRYRNNQHFEYIDIVREIGNAFTDILDASQSKDFASVIFTFISNE
                     NDKFHLTFFSRGMHFPFIARRFENSLMDIYNLNNREKGWIPAKSHLLGSEIRDLLGEK
                     YNKYISCHFVIYEGDSLFFFSDGLIEGTAIDNPHEEYGTERLMDCLREHIHLYPQAIV
                     NTMYEKIYEFIGRPERQFDDMTAVLIDFPLVRAN"
     gene            7855..9411
                     /gene="malQ"
                     /locus_tag="P5123_03750"
     CDS             7855..9411
                     /gene="malQ"
                     /locus_tag="P5123_03750"
                     /EC_number="2.4.1.25"
                     /inference="COORDINATES: protein motif:HMM:NF011080.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004134 - 4-alpha-glucanotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0005980 - glycogen catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-alpha-glucanotransferase"
                     /protein_id="HRX15406.1"
                     /translation="MQKTERNTGLLLHITSLGGDYGIGSLGKNARLFIDYLNQTGVKY
                     WQILPFNPVVRNFGYSPYASTSTFAGNPLFIDCADVIDMFDFLSVDMLPPVMEPSDFI
                     SFETVDDEHNRFFSRVYHAFSQKASLQHKDEFNLFCESNSTWLDDYGLFTVLSDIYQT
                     NSWINWPEKYARRDLGSITKAFEEFKDGINQVKFIQYVFFKQWENFRKHCKEKSVQII
                     GDIPIYVSYDSSDAWANPELFLLNSKGIPDPVAGVPPDYFSKTGQRWGNPIYRWFKDD
                     KPNHEVFEWWHLRVKHLLKITDLIRIDHFRAFESYWAIPEKEETAVIGSWEKGPGRLL
                     FDYLKEKMGNLPFIAEDLGIITKEVVALREELGFPGMKVLQFAFDQNPDNDYLPHSLQ
                     DTNTVLYTGTHDNNTSNGWFYGEDINKETREYIIEYLGMDSDSCFHKKLIKTALRSTA
                     ETVIIPVQDLLGFSSEFRMNTPGTMKHSNWRWQLKDINRIYEESWFLKRCNAIYNRLK
                     PVDHKSKEPS"
     gene            complement(9687..10277)
                     /locus_tag="P5123_03755"
     CDS             complement(9687..10277)
                     /locus_tag="P5123_03755"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GGDEF domain-containing protein"
                     /protein_id="HRX15407.1"
                     /translation="MQQRTMELAKRSEELEIALQNVQQANKKLEVLAVMDELTGLYNR
                     RAFMTITKQNIELKNRKKSDVLLVFFDLDGLKQINDNFGHSSGDIAIKAFSDILSKTF
                     RKSDIIARLGGDEFTVLAIDCTIKEYNTILKRIDKSINEYNLKSGNKFKLAMSSGAAP
                     CKDSDQCTIENLMEEADAELYKAKRKKKNTRDKISP"
     gene            complement(10486..10908)
                     /locus_tag="P5123_03760"
     CDS             complement(10486..10908)
                     /locus_tag="P5123_03760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000626853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system HicB family
                     antitoxin"
                     /protein_id="HRX15408.1"
                     /translation="MIKYAAQIVYDKEDNCYNVSFPDLKGCFTYGETMEEAQHNAKEA
                     LSGYLESLDMRKIKIPVPSKHNGKTLYYIKPEKNVSFAIWLKLKRQEKGYTQKDMANI
                     LNIKYQTYQRFENTQQSNPTLKTITKIENVINEEIIRV"
     gene            complement(10905..11084)
                     /locus_tag="P5123_03765"
     CDS             complement(10905..11084)
                     /locus_tag="P5123_03765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009001994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003729 - mRNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system HicA family
                     toxin"
                     /protein_id="HRX15409.1"
                     /translation="MTGKEIINLLKKSGWKLDRINGSHHIMVKDCDTIVIPVHGKRDI
                     PKGTLNQILKQGGLK"
     gene            11302..12426
                     /gene="aepY"
                     /locus_tag="P5123_03770"
     CDS             11302..12426
                     /gene="aepY"
                     /locus_tag="P5123_03770"
                     /EC_number="4.1.1.82"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004072699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033980 - phosphonopyruvate decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0017000 - antibiotic biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonopyruvate decarboxylase"
                     /protein_id="HRX15410.1"
                     /translation="MKVDKLLGFAEDLGIDFFTGVPDSQLKALCDTLFDRFKSSDNHI
                     VAANEGAAVALASGHYLATGKPALVYMQNSGIGNAVNPVCSLTNDKVYAIPMLFVVGW
                     RGKPGVHDEPQHVFQGEITVKLLELLEIKTFILTKETSDSDFETIGNEIKKVLASGKS
                     AAIVVEKDALQTDVKPDFNNSFSMTREEALGVIIEKSEDSDIFVSTTGKMSRELFETR
                     ERLNQGHSKDFLTVGSMGHSVMIAAAISLNKKNRRVFCLDGDGALLMHGGSLAVNGTL
                     ACPNMVHIVINNRAHESVGGMPTAAAHIDLVAMALSCGYKKSFRAENEEELSAILNKI
                     NKTEGPVFVEVNTNIKSRKELGRPTTTPVQNRDAFMNFLK"
     gene            12448..14466
                     /gene="aepX"
                     /locus_tag="P5123_03775"
                     /pseudo
     CDS             12448..14466
                     /gene="aepX"
                     /locus_tag="P5123_03775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009537382.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0050188 - phosphoenolpyruvate mutase
                     activity [Evidence IEA];
                     GO_process: GO:0019634 - organic phosphonate metabolic
                     process [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate mutase"
     gene            14476..15579
                     /locus_tag="P5123_03780"
     CDS             14476..15579
                     /locus_tag="P5123_03780"
                     /inference="COORDINATES: protein motif:HMM:NF012678.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonoacetaldehyde reductase"
                     /protein_id="HRX15411.1"
                     /translation="MTKKQHLITGKDGYKKINEILQKHGSAKFMLVCSKRNNFVKQYL
                     KESKLNHVIFSDFQSNPLYKNAVAGVSLFKKEKCDFVIAIGGGSAIDIAKCVKLFCKM
                     DDSKNYIDQQYMDTEIPLMVLPTTAGAGSEATRYAVIYRDDVKQSVTHNSIVPDYVIL
                     EPAFLSTVPDYHKKSAMLDALCQAIESMWSVNSTEESRDYSRSAIKLILENYKAYLAD
                     DQNAASDIMYAAYLSGRAINITQTTAAHAMSYKLTSLYRIAHGHAVALCLPHVWLYIF
                     ENIDKTTDPRGISHLRDILTELDTLFSSDDTSETIEQFYAFVKSMELKPFGNDLEILV
                     NSVNTDRLKNNPVNPGTDGISEMYKTIMKEIIR"
     gene            15634..>16855
                     /locus_tag="P5123_03785"
     CDS             15634..>16855
                     /locus_tag="P5123_03785"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRX15412.1"
                     /translation="MKLPAFAFKTIIALSRLLPFYLLLYKDNRQQILKDLYYNYHHKY
                     DKLLTKIKKNGFGNISCNDLKTIIPFLEKAGYHEELQKSLSEAIKKYPDNSFFNMYFA
                     RFYHFKNNIDEAFRMATKSLELDSQNTQSAALMITLKYINDKRNKADKFALELLTKYP
                     LNIKVLLSVCYRCASKDQFKAILEIWQTALKKGNNSNKLYVQTVPHLVTAAARANMFK
                     EGIDLSFKASLMILQKKVILTKFPKSLRNKYSDIAIRDICEVFNSCKVPFFFAAGTAL
                     GFIREGKVLEHDADIDVGIMEKDWAADIIMDAFNRHPHFLLEEPHPDNPKIGLVHCGG
                     ADIDIFRFYKENDSFYHNGGFVRWKNSIFNTKKYSFDRAEFFLPDPADKYLTENYGNW
                     KLPDPQFDAFLYAPN"
BASE COUNT         5542 a         2950 c         3125 g         5238 t
ORIGIN      
        1 tttacgcccc cgttttctgt caatcgatat tcaaaatctt tttttaaaaa atcctaatca
       61 tggaaccaag ttatgaacga tctttacaag aattactgat aacaacatgt aattttatcc
      121 gcattatttg aaaaggtttt aaagcaaaaa gactgattaa gccgactata tatatagtga
      181 cttatacttg attttaataa attattattt gtgaaaaata actcaagtga aaagggagat
      241 aatatgaaaa tacaaaaaat acaaaaaata ctatcgattt ctttgatact ggttttaaca
      301 gtcttaatgt cttgtgaaat agaagatgat aatgacgaga attcaaataa cgggttagat
      361 tttcgtattg ctgctgttaa tgtaaacgga atcgtttccg atacaaattc tgaaacctac
      421 tcttataatg atagaggcta tttaacaaca ttactggtta aggatggaga tggaaacaca
      481 attagcatga cttcctttga atattcttca gaaggattat tggttaaaaa ggttcaaaca
      541 agcggtggag aaactgtaat aacgcaaaat gagtatgacg agaatcgaaa gataagatct
      601 actatgaaag atcaaagtga aattatacta ggaatcataa atttttatta tgatgataga
      661 ggacgtatcg caaaaaaaga ggagaaggat gcatcaggtt ttataaaagg aacggcagat
      721 tacatttatg aagagggtgc tccaaagggt gattctgaag atataactct cagaatatta
      781 aaaaatagat aatttttata tatatgttca cttttgttaa tagtcaaatt ccgggacaaa
      841 attatttcct aatgtcgcgg aaatcatcat cactcatcat cactcaatat tattcactaa
      901 aataccttac aggcattacg taaaatgcgt tgtactttcc ccaatagtat tgtttaccct
      961 tatttctttc ataagtagca atattccatg cataatgtat cgaatagctt gtagaactcc
     1021 agtaatgatg gttttcgttt tctatcagac ctttagaaat caagtttgta taaatcattt
     1081 ccaattcatc tttagaaggc aagaaccaat catcttttcc gcctcctcgg taagaatgcg
     1141 ccatttttgc agcaaatatt ttttctgaac aatgctcaac atgattaatg gtattttctc
     1201 ggccggttcc tacggtcttc ccacctgttc ctcccatcga ataacgataa cacccccaaa
     1261 cagctcttcc tacagctttt ggagcagcct ccatatagcg ccaaccgtca ctattgtctc
     1321 ctttatcgta aaaaacgatg ccaccagccg ggcctatgtc tccaattgaa tatccatttt
     1381 tgctggcacc caaacgatta attataaaca atgacaaaac taaaaaaaag agaataataa
     1441 aaaatcgcct acttacaatt ttttctacaa attccataat aaatacctca taaatttgaa
     1501 ttaatggtta atatttatat agaaatgtgc aaaattatgg tgaagttata tttaaccaac
     1561 cgcacaccca aattgtaata aaatttttaa ccatttatgg gatatttata catctaatta
     1621 cagatattgt aaatgggaca aaagttctaa ttgggtaatt taatcatcta attatcacgg
     1681 caacagttaa tcaggtcgat tctgttttca gaatctgttt tttgaaacat gctataaaca
     1741 tgttttttaa cagtagaatc tgatatttgc agatttgcag ctatctgatt gttggtgtaa
     1801 cctttcaata tcagataaaa aacctctttt tctctcaccg taaactggta ctttttaagc
     1861 atataagaaa tattttctga tatatctgat ccgatcttaa ttagataatg atacgaacgg
     1921 gggataacaa gaaaagaaat caaaagaggg ataaagatgg aaaaattgaa gccttgctct
     1981 tttaaaaaaa tgaacttagg aaaaaaaggc caaaagatat ccagtatacc aggaaccagg
     2041 tatccaatgc ctaaaataaa cgtataaaca agatacgatc tttgaaaaag ggtcttatta
     2101 taaagattaa tcgccattag tatcaaacaa tacatgtgca caattaaaaa aagagcccca
     2161 caaactataa ctgcataaag aaaagaggat tgatatgttc catcaaaata gaaaataata
     2221 aacggcaatg aaaatatttg aataatagaa attggagtaa caaacattgg aatttttttt
     2281 tcaagatagg tgtgaattaa gcatggtaaa gctaatgtag ttattacaag agatataata
     2341 tctaaaatag atgcggttat ggtaataccg gaattattta ttccataatc agccagatat
     2401 tgaacactca agaaaagtct tgaaaatatg gataacaata cagcaaacat gtataccaag
     2461 cctgccttca atgttatcaa atgatatttt gataccaagg gagtaaagaa acatatagca
     2521 cagcagaaag cagtaaaaac tattaaattt aaccataata cgatagtgct catattatca
     2581 caatcctttc agataaatag agatcaacta atatgggtga taacgggtta tcgtgtcaag
     2641 cacgagagca aatgattaat caaaaaggga ttgccgctta ggcaatccct ttttgttttg
     2701 ataatcaatc aacaaaattt tacttagtat tacatggcat aaagatcaat agatgagaca
     2761 tcaggtatgg cgattcttgt ctcaagattt tttttatcaa acatgactat ttcgtcttca
     2821 ataagatctc tgtttttatt aagttctatt gatttgccgt ctttaagatg cactacgata
     2881 tcaagccctt taattgaaat gtacttatcc aacatttctt taaatcccat cggttttttt
     2941 tgccgggttt tcatcattcc tcctgtcgta ttatgacaat tatgacatac tgcattataa
     3001 atattattaa catctggttg taaaccgctc agaagggttt aaacaaaaag ggttaaatat
     3061 tttaccgcaa ggatgtctgt attattaaga tcacaatcag ctgtattatg tctcattaaa
     3121 attatcggca ctttcctgtt tttgttaagt aaattgtctg taatatcaaa aaaaattgaa
     3181 attaggagca accgcatgat ttaccgatat gtttattgta gtttacaaat gcaacaatag
     3241 aaaagaaaac ggtgagtgcc gtaaaggtaa ttctttgtaa ttcggaatct attgacataa
     3301 ttgggaaatc caatactatt aaggtatccg tataaaaggt taaggagtca gttatgggta
     3361 aaattacacg cacatatatt tttttcatta tgtttttttt aggttgtagc tctaactatg
     3421 ttcaatcaac aaacaatatt gctaaattca aaacagaagt tattccatac gataagtctt
     3481 tactattgtg taaaatcaca ggcaattcca tggacagtgc cctatatata aaaagttacg
     3541 agttagtcta taatatagat aatgttcata tttacataaa taagagtttg aaaaaaacag
     3601 gtatcagttt tccctatttt atacagttta ttatatctaa aaacataaat tacgtttatc
     3661 ttggtgattc tgaaataata tggagaagag aataggtata tgagtaatga gaggccggag
     3721 tcgtgaaaca gaacagtaaa gtacgattga attgtacaga tgaacgcgca gaaatcatac
     3781 ataaggttgt tgagtaccat aatgaataaa accatattaa aagagtttaa catacgttat
     3841 aaagccgata aaagatcaag atccgtaaaa atcagagcgg ccgaatttaa tactttggat
     3901 attacttacc ctccgtcact ttctttaaat gtcgtaaacg atattattgt aagccgtgca
     3961 gattcgatac gtaacgccta taacaaagtt aaggccagta gcatgtctgt agacagcttt
     4021 gttcgcgaaa aaaataattt attacgtatt gcagactcag ttctctattt tgaaaaaagc
     4081 agtgacccta aacttaacgg agtaaccaag atcaaaaatg gtgatcgttt tttctatttt
     4141 agcaaaaatg atggtttaaa agatcccgat acagctcgcc ttgcggcaaa atcaatcatt
     4201 atgttttgta caaaatgcgc aaaaaaaata ctgccacaaa cagttaaaaa acttgcggat
     4261 aaacatggtt tctcttataa taatctctcc tttagaaatc aaaaaaggcg ttggggaagt
     4321 tgctctcacg acaacaattt atctttaaat gtcagactca tgcttttgcc gtataaactt
     4381 attgaatatg tgctgataca cgagcttgta catacgatac acaaaaatca cagttcgaca
     4441 tattgggacg ctgttataag aattttaccc gacgctaaac tgttacgtaa acaattaaaa
     4501 aaatatgact ttaccttctt agaccgaaac aactgaatat aacaaaactt acgtggagcc
     4561 ataatgaact ttttacgctt tgtctattat tttctaagac gttactatga ctctgttaat
     4621 tatgctatag agggaatttt acacgccgcg aaaactcaaa gccatgtaag gttccatctc
     4681 tccgcggcat gtttccttct gcttgtctgt ttttcgatcg gtatttctcc gtcagaattt
     4741 gttattttga caacacttgc aacaattgtt attgtcgcag agatgcttaa cagcgcgatc
     4801 gaagtaactg tagatattgc ctctcccgag cacagtgaaa aagctcgaat cgcgaaggat
     4861 attgctgcgg gggcggttct ggtggcggca gcagctgcag cgattatcgc tttttttatt
     4921 ttttatccct attttcacta ttttattgag tacggaattg ttatagcaca acatggtgtg
     4981 gttgatctta tttttggttc aaccatgatt gtactgataa ccgtaataat gatgaaagcc
     5041 tatgttggca aggggcatcc tcttcgcggc ggacttcctt ccgggcatag tgctcttgct
     5101 tttacctggt gggttaatat cgtttttatt tacaaggact tttatctgac agttatattc
     5161 tttgcatgtg ccatcgcgat tgcggtaagc agggtggttc ggaaaataca tacgatttac
     5221 gaggtttgta ttggcataat gaccggaaca gttataacct gggggcttta cctgatattt
     5281 tataaaggtt agtttttaga accttcttct atttgatgca tttgttgcat aatttcttcc
     5341 ctttcagatg agaaaagaac agtacgccat gcccaatctt cggaatattt gtagtagtgc
     5401 gtggcaaggc ctattgcctc gtattggggc acatatttgc ttcttccctt cgaatcacga
     5461 attttcacat caccctgata cacgcaaaaa gcaatgctgt tgcgtattag cctgtcaaaa
     5521 agttgttggt cacctgttct ttgaagcaac gcgaaaggcc gtcttgcgta aaaggtaagc
     5581 ctgtaatcgt catctgcagg taaaaaattt atcgtatttt gagattcact ggctgtaaac
     5641 tggtcaaaat ccgaaagaac aattaccttg ttactgtaaa aatagggacg tgaaattagc
     5701 agaataaacc ggattcttag ccagccgata tattcggact cgagaagatt taagcgcctt
     5761 ttatcatcag tactgataaa agatatttcc tgtttttttt tgttaagaac agaatttaaa
     5821 gagaaccgtt cagaaacgtc aattaacctg tagccagatt tttcatgcga aaactgggtt
     5881 ttacgcccgt tgattacggg taaaacctta taatagtatt ccacaccggg ttcgcagttt
     5941 gtatcggtgt aatttgtttt ttttgttacg gtagctatac ggtcgtattt accttttttg
     6001 tattcggatc tataaatatc atatctgtcg gtaccctcgt aggcagtcca acgcagagga
     6061 ataccaaaat atgacttgcc gtgtgaaacc tgcaactcta ctatctcgtt ttgagataaa
     6121 gcaggctgca atacaagcag tatggcaacg atatgaaaaa atcctgattt aatcattaat
     6181 tccttgttta aaagtaaatt attgaggtcc ctgcatataa gactttacca ataacttatt
     6241 accctatgac agtcaaaaca attttttttc acgaaactct ttttgtagtg aacttagaaa
     6301 aattacaatg tttcaaaagg aacatggatt aagagtcgat tctatagtaa tttaatttac
     6361 gaccgataat caaaccaagc gcataaaaaa acggagaaaa caatggatta ttcacaattt
     6421 agtcaactga atgaccaaac ggatatggat attatcgagt ttggccagca gacctatttg
     6481 atcggttgca aatatgatga aatagccagt tctcttttaa aaagatgttc cgacaaaatg
     6541 tttgagcatg aggccgatat ttctgtttgt gatggtagca attgtgcaat tttcaggtca
     6601 aaaagtcttt atatcgatgt taaaacaggt gaagttattg tacaaacaat cgatggcaaa
     6661 tcggcacctt ttaaaatgct aatgatagag gcaaaagaga tgctgataaa ctatctgcat
     6721 attcatgtca aaactgctga tatacataac aattttttta atagctaaaa gctcatttga
     6781 gagaacttcg aaaaattaca attttctaaa gggaaattta taagagattt tcttgacact
     6841 ttttgtgtat taaaagcaag ttaaactcag gaaacaaaga atagggtaat atgtcagata
     6901 ttcaatacaa attgatgaaa aatgcgaatc tctctataga ggatattatt cgacagtact
     6961 caatgataga ggctgaagcg gaagaggttc gtaataatga taagctgaca gtaaaaaacc
     7021 tttttggcag tttttatgga aagaaactga atgtagggat aggttttcaa cgcgcgtatg
     7081 gatcgatatt atcgggtgat tatttcgagc tttacaaact tcctgattcg aactatctct
     7141 ttgtttttgc cgacatcagt ggtcacggtc ttccagccta taccactctt atcaggttaa
     7201 gatccgctat tacctcgaca cttaaaatgt acgaaaaacg ctacagaaac aatcaacatt
     7261 ttgaatatat tgatatagtg cgtgagattg gtaacgcctt taccgatatt cttgatgcgt
     7321 cacaatcaaa agactttgcc agcgtaatat ttacttttat aagcaacgaa aatgataagt
     7381 ttcaccttac ttttttttcc aggggaatgc atttcccttt tatcgcgcgc cgctttgaaa
     7441 actctttgat ggatatttac aatcttaata accgggagaa ggggtggata ccagctaaaa
     7501 gtcacctgtt aggctcggag ataagggatc ttttaggcga aaaatacaac aaatatatat
     7561 catgtcactt tgtaatatac gagggtgatt ctctcttctt tttcagtgat ggcttaattg
     7621 agggaaccgc tattgacaac cctcatgaag aatacggcac tgaacgtttg atggactgtt
     7681 tacgcgaaca catacacctc tacccacagg ccatagttaa tacaatgtac gaaaagattt
     7741 acgaatttat cggacgtcct gaaaggcagt ttgacgacat gacagctgta cttatagatt
     7801 tccccttagt gcgtgcaaat taaaaaatta ttatcggggt tctcgtaaaa agatatgcaa
     7861 aaaactgaac gcaacacggg tcttctttta cacataacgt cacttggcgg tgattacgga
     7921 atcgggtcac ttggtaaaaa tgcaaggctc tttatagatt atcttaacca gacgggagtc
     7981 aagtactggc aaatacttcc ctttaatcct gttgtacgta attttgggta ttcaccctat
     8041 gcatcaacct ctacctttgc gggaaatccg ctgtttattg attgtgccga tgtaattgat
     8101 atgtttgatt ttttgtctgt ggacatgctt cctccggtca tggaaccttc agattttatc
     8161 tcgtttgaaa cagttgatga tgaacataac cgttttttta gcagggtata tcacgcattt
     8221 tcacaaaaag cttcattgca gcataaagac gagtttaatc ttttttgtga atcaaacagc
     8281 acctggcttg atgattacgg tctgtttacg gtactttcag atatttatca gaccaacagc
     8341 tggataaact ggcctgaaaa atatgcgcgt cgtgatttgg gatctattac aaaagcattt
     8401 gaagaattca aagatggtat taaccaggta aagtttatcc aatacgtttt ttttaaacag
     8461 tgggaaaact ttaggaagca ttgtaaagaa aaatctgttc aaataatcgg cgatatacca
     8521 atctatgtca gttacgacag ctctgacgcg tgggcaaatc cggagctttt tcttttaaac
     8581 agcaaaggca ttcccgaccc tgtcgccgga gttccacctg actattttag caaaaccggc
     8641 cagaggtggg ggaaccccat ttaccgatgg tttaaagatg acaagccaaa tcatgaagtt
     8701 tttgaatggt ggcatttgcg cgtaaaacac cttttaaaaa ttaccgactt aataagaata
     8761 gatcattttc gtgcttttga atcttactgg gccatacccg agaaagagga gactgctgtg
     8821 attggtagtt gggaaaaagg tccaggacgt cttttgtttg attatttgaa agaaaagatg
     8881 ggtaatttgc cctttattgc agaagatctt ggaataatca cgaaagaggt cgtagcactt
     8941 cgcgaagaac ttggatttcc aggcatgaaa gttttacaat tcgcctttga tcagaatccg
     9001 gataatgatt atttgcctca ttcgttacaa gacacaaata ctgttttgta taccggaaca
     9061 cacgataaca ataccagtaa cggctggttt tatggcgaag atattaataa agaaactcgg
     9121 gaatatatta ttgaatacct tggcatggat agtgatagct gttttcataa aaagctcatc
     9181 aagacagctt tgcgcagcac agcagaaacg gtaataatac cggttcagga tcttcttggt
     9241 ttttcatccg agtttagaat gaatacgcca ggcactatga aacattcaaa ctggcgttgg
     9301 caactaaagg atatcaatag gatttacgaa gagtcatggt ttttgaaaag atgcaatgcc
     9361 atttacaaca gattaaaacc tgttgaccat aaaagcaaag aaccatcctg attattgtac
     9421 aatttaatta tatcacataa ttatttcata tttatttttg gaacgaaggc tttgattgat
     9481 aagagcgtga ccttctaaaa attactttta acagaacagc tatgttccgc aaaggtgagc
     9541 ctgtaaaatt ttctaatgag acataagata tcttatcgga agttacatat tgcctcaagt
     9601 aaaaaggtac tccaaacaca tcttgttgcc tcatagaaaa aagtactcca aacttttcta
     9661 acttgtttat ttatttcaga gcgattctat ggtgaaattt tatctcttgt attctttttt
     9721 tttctttttg ctttatataa ttctgcgtca gcttcttcca ttaaattttc tatagtacat
     9781 tgatcagaat ctttgcatgg agcggctcca ctggacattg caagtttgaa tttgtttcca
     9841 ctttttagat tatactcatt aattgattta tcaattcttt tcaaaatggt gttgtattct
     9901 ttgatggtac agtcaattgc aagtacggta aattcgtctc ctccgagtct tgctatgata
     9961 tccgattttc tgaaagtttt tgaaagtatg tcagaaaaag ctttaatcgc aatgtcgccg
    10021 cttgaatgtc caaaattatc attaatctgc tttagaccat ctagatcaaa aaacacaagt
    10081 aagacatcag attttttacg atttttcagt tcaatattct gtttggttat tgtcataaac
    10141 gcgcgacgat tgtataaccc ggtaagttcg tccatcacgg caagcacttc aagtttttta
    10201 ttagcctgtt ggacattttg caatgcaatt tccagttctt cagaacgttt ggcaagctcc
    10261 atggttcttt gttgcaccat ttgctccagt ccttcagaat gtttcttaat attttttagt
    10321 agacgagctc cttgcaatgc acccgataag tgatcactta gtacttctag taccgggaaa
    10381 taaatctatc aaccggtaca ttaaatagtt tcgctagtaa cttggcgtta ttaatactaa
    10441 ttgatcttat tcctctttcc atataagtaa accaataaag ggtctttaca ctctaataat
    10501 ttcttcattt attacatttt ctatttttgt tatggttttt aatgttggat ttgattgttg
    10561 tgtattttcg aatcgttgat aggtttgata ctttatattc aaaatatttg ccatgtcttt
    10621 ctgcgtataa cctttttctt gtcgtttaag ctttaaccat attgcgaaag agacattttt
    10681 ctctggttta atataatata atgttttacc gttatgtttt gaaggtacgg gtatttttat
    10741 ttttctcata tcaagtgatt caaggtaacc gcttaacgcc tctttagcat tgtgctgcgc
    10801 ttcttccatt gtttccccat acgtaaagca tcctttcaag tcgggaaaac taacattgta
    10861 gcagttatct tctttatcgt atactatttg cgcggcatat ttaatcattt tagcccacct
    10921 tgtttcaaaa tttgattcag tgttcctttt ggtatatctc ttttgccgtg tacagggata
    10981 acaatagtat cacaatcttt gaccataata tgatgtgaac catttatacg gtcaagtttc
    11041 catccactct tttttaataa attaattatc tctttaccag tcatgattaa acatacagct
    11101 taatagctgt attgtcaata ataaagctgt aaaatttatc tttgaagtaa acttatctat
    11161 tataaatgat aaataccatc ttctatggtg aagtaaatgc ttccggtttt tatcttgaca
    11221 gtaaccatat ttgacaaatc agtaatatat tctaaaaagt ggttttgaca cataaaaaat
    11281 agttaatttg gagtattatt catgaaagtt gacaaacttc tggggtttgc cgaagatttg
    11341 ggaattgatt tttttacagg agttcccgac tctcagctga aagcactttg tgacaccctc
    11401 tttgacagat ttaaaagcag tgataatcat atagtagccg ctaatgaagg cgccgctgtt
    11461 gctcttgcct cgggacacta ccttgccaca ggcaagccgg cactggttta tatgcaaaac
    11521 agcggaatcg gtaacgcggt gaacccggtg tgttcgctta caaacgacaa ggtttacgca
    11581 ataccaatgc tttttgtagt tggttggcgc ggcaaacccg gagtgcacga tgagccgcag
    11641 catgtttttc agggtgaaat aaccgtaaaa ctgcttgagt tattagaaat taaaacattt
    11701 attttaacaa aagagacctc ggacagtgat tttgaaacca taggtaatga aattaaaaag
    11761 gtactcgcta gcggcaagag tgccgcaatt gttgtggaaa aggacgcgct gcaaacagat
    11821 gtaaaacccg attttaataa cagcttcagc atgacacgcg aagaggctct tggtgtaatc
    11881 attgaaaaat cagaagatag tgatattttt gtttcgacaa ccggcaagat gtcgcgtgag
    11941 cttttcgaaa cacgcgaaag attaaatcag ggacactcga aagattttct gaccgttggt
    12001 tctatgggac acagcgtaat gatagcagct gccatttccc ttaataagaa aaaccgacgg
    12061 gttttctgtt tggacggtga cggggcgctt ttgatgcatg gcggaagcct tgctgtaaac
    12121 ggcaccctcg cctgccctaa tatggttcac atagtaataa ataaccgtgc acacgaatct
    12181 gttggtggaa tgcccactgc cgcagcccat attgatcttg ttgctatggc gttgtcttgc
    12241 ggctataaaa aatcgtttag agccgaaaac gaagaggaac tttcggcaat acttaacaag
    12301 ataaataaga cggaaggccc cgtatttgtt gaagttaaca caaacataaa atcgcgtaag
    12361 gagcttggac ggccgacaac tactcctgtt caaaacaggg acgcgtttat gaatttttta
    12421 aaatagatct ggagcaaaat aataatgatt aaggtactta tcttgaactc cggtttaggg
    12481 aaaagaatgg gtaaacttac cgaagaccga ccaaagtgca tggtaactgt caatgacggt
    12541 ttgagtacgc tagattttca attgaaaatt attgaaaaaa aaggaatcag tgaagtggta
    12601 atgaccactg ggccttttga agagaagctt attgatcata ttaagacaaa ctatccttct
    12661 ttgaatgtcc gatatgttaa taaccctgat tacaaagaga ccaactatat ctattcgata
    12721 gcgttagcca aagaatttct ggactctgat attgtttcga tgcacgggga tcttctattt
    12781 gaagagagtg tttttgacga tattctggaa agccgtaaaa gtgtgatggt taccgacagc
    12841 ggacttcccc tgcccgaaaa agattttaaa gccgttgtcg cggataagcg tataagtaaa
    12901 atcggtatcg atttttttga taatgccgtt tcggcccaac cgctttataa gctgcttaaa
    12961 aacgactgga gaatctggct ggacgaaatt atgaaatttt gttcaaatgg taaaagatcg
    13021 gtatacgcgg aaaacgcgtt taacgagatt tcggatcaac tgcaactgga aacactggac
    13081 ataaagggcc gtctttgttg tgaaatagac accgaagaag acttgctaaa aatcagagaa
    13141 attatttaaa aaaggaatat ttacaagatg aaaaaagtat atttaagcat gagttcggac
    13201 attattcata acggacatat aaaaataatc tcaaaagcat ccgcgctggg agagcttact
    13261 gtggcagtat taaccgacga ggctgttgcc aagtacaaac gttaccccat gataccattc
    13321 gaagagcggg ttcagataat cggaagcata aaaggtgtta caaatgttgt agagcaaaca
    13381 acgatagatt atacagaaaa ccttttaaaa tataaacctg atattgttgt tcatggtgat
    13441 gactggaagg agggttttca gcagccgatt cgcgaaaagg ttatcgaaac ccttaaaacc
    13501 tatggcggcg agcttgtaga gttcccctac acccaagaag agaaccttat agctattgaa
    13561 aaaaacgcgc gcaaaatact ctccatgccc gaaaagcgtc gcccaagact tcgtgaactg
    13621 ctaagacata agggctgcct ttcgattatg gaagcacata acggccttac tggtttaatt
    13681 gtcgaagata ctcaggtcca aaacaatggt gaaataaagc aatttgacgg aatgtgggta
    13741 tcaagtttat gtgattcgac agccaaggga aagccggata tcgagcttgt agacacaact
    13801 tcacgattga aaacgattca ggagattatg gaggttacaa caaagcccat aattcttgat
    13861 ggagatacag gtggtcttgt agagcatttg cagttttttg taaagacact cgaaagaatc
    13921 ggtgtttcgg caattataat cgaagacaag gtaggcctta agaagaattc cctttttgga
    13981 acagaagtcg agcaggtaca ggctcctatt tgtgattttg ccgaaaaaat tcgaataagc
    14041 aaggcttcgt tatcgactac tgactttatg ataatagcgc gaatagaaag ccttattctt
    14101 gaacagggaa tggaagatgc tcttaaaaga gctttcgcct atgtagaggc cggtgctgac
    14161 ggtattatga ttcattcacg tcagaagact cccgacgaga ttatggaatt tataaaatct
    14221 ttccgtgcaa aagacaagca aaccccgatt gttattgtgc ctacaacatt taattcggta
    14281 accgaatccg aatggcagaa tatgggagta aatataatta tttacgccaa ccagttgata
    14341 cgaagcgctt tcccggcaat gaaaaagact gccgaaacca ttcttaaaaa taaccgttgc
    14401 atggaagccg acgaggcgtg catgccaatt aaagagatat taacattaat acctgaggca
    14461 gaataggata tcccaatgac aaaaaagcag catttaatta caggtaaaga cggctacaaa
    14521 aaaattaatg aaatattgca aaagcatggt tcggcaaaat ttatgcttgt atgctctaaa
    14581 cggaataatt ttgtaaaaca gtacttaaaa gaatcaaagt taaaccatgt gatattcagt
    14641 gattttcaga gtaaccctct ttacaaaaat gctgttgctg gtgtctcttt atttaaaaaa
    14701 gaaaagtgtg actttgtaat tgccattggc ggaggaagcg cgattgatat agccaagtgt
    14761 gttaaacttt tttgtaaaat ggatgattcc aaaaattata ttgatcaaca atacatggat
    14821 acggaaattc cgctaatggt tttgcctaca actgccggag ccggcagtga ggcaacaaga
    14881 tatgctgtaa tataccgtga cgatgttaaa cagtcggtta ctcacaactc tattgttccg
    14941 gattatgtta ttcttgaacc cgcgtttctt tctactgtac ctgattatca caaaaaaagt
    15001 gcaatgcttg atgctctttg tcaggcaatc gaatcgatgt ggtcggttaa ctcgacagaa
    15061 gagagccgtg attattcacg aagtgccata aagctgattt tagaaaatta taaggcgtat
    15121 ttagcagatg atcaaaacgc cgcaagtgat attatgtatg ccgcctacct tagcggaaga
    15181 gcaattaata taacacagac aaccgccgca cacgccatga gctataaact gacatcgctt
    15241 taccggattg cccatgggca tgcggtcgca ctctgcttgc ctcacgtttg gctctatatc
    15301 tttgagaaca ttgataaaac aactgatcca agagggattt ctcaccttag agatatttta
    15361 actgaacttg atacgctttt cagctcggac gatacgtcag aaaccatcga acagttttat
    15421 gcttttgtta aatctatgga actaaagcct ttcggaaatg atcttgaaat tcttgtaaat
    15481 tccgtaaaca ccgaccgcct taaaaataat ccggttaatc ctggtacaga tggtatttca
    15541 gaaatgtata aaacaataat gaaagagatt atacgataaa aatttttttt tataaaaaat
    15601 ctttaaattt ttattttctt aaaaggaaat tcaatgaaat tacccgcgtt cgcgtttaag
    15661 acaatcattg ccttaagtcg acttttacct ttttaccttt tactatataa agataacaga
    15721 caacaaatac taaaagatct ttattataat tatcaccata aatatgataa acttcttaca
    15781 aaaattaaaa aaaatggttt cggtaatata tcttgtaacg atttaaaaac aataattcca
    15841 tttcttgaaa aagccgggta tcatgaagag ttgcaaaaga gtctttcaga agccataaaa
    15901 aagtatcctg ataactcttt ttttaatatg tatttcgcca ggttttacca tttcaaaaac
    15961 aatattgacg aagcttttag aatggcaact aagtcactgg aacttgactc gcaaaacaca
    16021 caaagtgccg cattaatgat tacacttaaa tacattaatg ataaacgtaa taaagccgat
    16081 aaattcgcgc ttgagctttt aacaaaatat ccgttaaaca ttaaggtttt attaagtgta
    16141 tgctaccgat gcgctagcaa agatcagttc aaagccattc tggaaatttg gcaaacagca
    16201 ttaaaaaaag gaaacaatag taacaagctt tatgtgcaaa cagtgcccca tcttgtaact
    16261 gctgctgccc gggctaatat gtttaaagaa ggtatcgatt tatcatttaa agcttcgctg
    16321 atgattttac aaaaaaaagt tattttaaca aaatttccca aaagtcttag aaacaaatat
    16381 tccgatattg ccattcgcga tatttgtgaa gtgtttaaca gttgcaaagt gccctttttc
    16441 tttgcagccg gcacagcact cggttttata agagagggca aggttctgga gcatgacgcg
    16501 gatatagatg tgggaatcat ggaaaaagac tgggctgctg atataatcat ggatgctttt
    16561 aacagacatc cgcatttttt acttgaagag cctcatcccg ataatccaaa gattggactt
    16621 gttcactgtg gtggcgcaga tatagacata ttcagatttt acaaagaaaa cgactcattt
    16681 tatcacaacg gaggttttgt ccgttggaaa aacagtatat ttaatacaaa aaaatacagt
    16741 tttgatagag ccgaattctt tcttcctgat ccggcagaca aatacttaac cgaaaactat
    16801 gggaactgga aactgccaga ccctcagttt gacgcatttt tgtatgcgcc gaata
//
LOCUS       DAOTGZ010000033        16580 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_3064
            NODE_4208_length_16580_cov_5.647020, whole genome shotgun sequence.
ACCESSION   DAOTGZ010000033 DAOTGZ010000000
VERSION     DAOTGZ010000033.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868088
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 16580)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 16580)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:22:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,785
            CDSs (total)                      :: 2,751
            Genes (coding)                    :: 2,732
            CDSs (with protein)               :: 2,732
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 12 of 19
            Pseudo Genes (internal stop)      :: 5 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16580
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4208_length_16580_cov_5.647020"
                     /isolate="AMR_MDS_3064"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            74..1147
                     /gene="prfA"
                     /locus_tag="P5123_03790"
     CDS             74..1147
                     /gene="prfA"
                     /locus_tag="P5123_03790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010253797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016149 - translation release factor
                     activity, codon specific [Evidence IEA];
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 1"
                     /protein_id="HRX15413.1"
                     /translation="MLEKLKALESRFKKIEQEMSDPDIIKDQTRFRDLNREHSRLSPV
                     IESFAEYKKAVSELADTKKMLEDERDSDMREMLAAEKRDLEEKIPRMEQDLTIMLLPK
                     DPHSGKNVIIEIRAGTGGEEAALFVSDLYRMYTRYADLKKWKIEIMDQNQTEIGGLKE
                     VTFLVSGADVYDELKYESGAHRVQRVPETESGGRIHTSAVTVAVMPEAEDTDVEIRQE
                     DLRVDVYRASGHGGQSVNTTDSAVRITHIPTGLIVTCQDEKSQLKNKQKAMKVLRARL
                     FELEEQRRQSEQSQMRKSQVGSGDRSERIRTYNFPQSRLTDHRIGLTLYSLESVLEGE
                     LEPVIVPLKEYEIEQLLKSNDTV"
     gene            1144..1968
                     /gene="prmC"
                     /locus_tag="P5123_03795"
     CDS             1144..1968
                     /gene="prmC"
                     /locus_tag="P5123_03795"
                     /EC_number="2.1.1.297"
                     /inference="COORDINATES: protein motif:HMM:TIGR03534.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA];
                     GO_process: GO:0018364 - peptidyl-glutamine methylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor N(5)-glutamine
                     methyltransferase"
                     /protein_id="HRX15414.1"
                     /translation="MTINSLISQCANRLRDAGIASYQLDARIIIAYVLDITSEKLLAE
                     GNSLVAEDHVARICSLCDRRQRREPLAYITGIKDFYSLKFMVTPDVLVPRPETELLVE
                     YLIDNCPKNSTVLDLCTGSGAIAVTLKYERPDLTVTGSDISVSALEVARKNCKFNSVD
                     KINFIESDLFEGITNKYDVIVSNPPYIPLSCKESLEPELGYEPQIALFSGEDGMDLPT
                     KIINQARDYLFSNGFLVMEIDSRSGEKIILAAKEKNMGCELMSDLAGYDRIAVIRI"
     gene            2002..3360
                     /locus_tag="P5123_03800"
     CDS             2002..3360
                     /locus_tag="P5123_03800"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRX15415.1"
                     /translation="MEYSLSDGTRPRFLVIKYILRVMAPYIFLLFFLIAINFGFNIYS
                     SWGGVNLVVKLYIYTFAFFLVYTISISLPPELRSAYSRYGLLTKFIYVLESRIFPFAL
                     IYIVTAIYSVVENMGSQYWPYDSFAGIFDDAISNLVIYSVVLMYVLKIKKRPYVTVPV
                     YLFLIYLYYMINQRFYQNFYAGNAMVAFKILKAALLFFLMTWSTKLKASRFFLNLCFS
                     LLLAIALNALIIGIYKLSYNNVSNRVLKNSIALKLTKFGRPSALSPLMKDVLDKKDIT
                     LLDQIYPYVKYYGKNFDISEIQWREFLFHDDVSFANRVASYMLKEGVSAHPAEIIDFV
                     KKNIKQDPDSIRNSEFLIRLTAVCLNEYGSNKELVEAMHMSDDVFLIWAIKVMGETGD
                     CHLISEIVVYLNHIDSDISDAAYNALKLLTGIDPYAQSQIPYNSVRSLKLFNDYLHKS
                     CK"
     gene            complement(3329..3955)
                     /gene="nth"
                     /locus_tag="P5123_03805"
     CDS             complement(3329..3955)
                     /gene="nth"
                     /locus_tag="P5123_03805"
                     /inference="COORDINATES: protein motif:HMM:TIGR01083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003906 - DNA-(apurinic or apyrimidinic
                     site) endonuclease activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease III"
                     /protein_id="HRX15416.1"
                     /translation="MESKADMLYKLLKKKYGETNPELTYSNLYELTIAVVLSAQTTDK
                     QVNKVTPELFLKYPDFKSLSNASLDEIEKIVHSTGFYHSKAKNILGLAKALSEKSFIL
                     PDSIEELIKLPGIGRKTANVVISQGFGKPGLAVDTHVGRIARRIGLTRSKNPDIIEKE
                     LKSMLAPEKWLEFHLLMINHGRKTCFARKPDCSICVAYSICNFYASNH"
     gene            complement(3955..4557)
                     /gene="rdgB"
                     /locus_tag="P5123_03810"
     CDS             complement(3955..4557)
                     /gene="rdgB"
                     /locus_tag="P5123_03810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017726680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016462 - pyrophosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RdgB/HAM1 family non-canonical purine NTP
                     pyrophosphatase"
                     /protein_id="HRX15417.1"
                     /translation="MIEIITASTNSHKIDEMNNIAGASAICNFTAMSRLNNYPKDIIE
                     DGNTFKENALIKAKALTEYTDKPIIADDSGLVIDALNGEPGIFSARYHGLESDDEKNR
                     LILELLKDVAEKERTARFVCLLAFIDSKRNTHFFEGTCEGMISKKIIGENGFGYDPIF
                     YVSEYKTTMANVAPETKNRISHRALALQKFLEFIKTMVQI"
     gene            4887..6848
                     /locus_tag="P5123_03815"
     CDS             4887..6848
                     /locus_tag="P5123_03815"
                     /inference="COORDINATES: protein motif:HMM:NF013736.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HRX15418.1"
                     /translation="MPEERSHFIVVLVYFLIKISLHNIVAFFCFIKPYSAVKDILLQF
                     QRGRYSMADSAHVLKPSACFGDLFELLRSTGRYLDNLVGSQNDEIEKFRELYNNVILS
                     ANSYFVVLNARDEVIFANKSFSANFRYTDDGILGLRLEDIFYIIPGRFSDAIDNVKKR
                     GQSAVLRKIKLISRRKISLIADIKISRVTVQGEKQYIVVMDDITSQWRKDYQISLISQ
                     ISESLQRDEEVERILYAILTAVTSGSGLGFNRAMLFLYNEQKKILTGKMAVGPDSMEE
                     AVQIWGSVQNEELNLLGGGPDAPPVLQTNGAFYKQVVTSNFAINGNTLFADVFNKRAP
                     VHIYDAASDPRVDPETRKFMDVSEFVVVPLCSANKALGVVVADNKFNGAPIYEDHVEL
                     LSIFAVQASLSIDSYNSLTSVKERMEHIQAKQEAIVESEKMAAVGRIAAHIAHEIRNP
                     LVTVGGYARRILQSVKKPQKTLEDIEKAASIILHESERLEKILSNVMDFSRPSPLIRE
                     YNNLNDVVKDTYYLLKNVLQERKITATLDLEQKLPLVKSDFNQLKQVVLNLLQNAMDA
                     TPAGGSIILKTGLTETHIYIRIKDSGTGISPEAMDKIFDPFFTSKITGVGLGLAVVKK
                     IVNDHNGEIVISNNSEGGCQFSVELLRPS"
     gene            6887..7252
                     /locus_tag="P5123_03820"
     CDS             6887..7252
                     /locus_tag="P5123_03820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012056688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HRX15419.1"
                     /translation="MSRILLVEDEKNQRELYAAELRDDGYEVEEASDGKMAVEKVKSS
                     KYDLVVLDIRMPEMDGIEALGKILSRDKKIPIVIHTAYSNYKSNFMTWTADAYVTKSS
                     NLDELKTKIAEILAARGQA"
     gene            complement(7258..7629)
                     /gene="queD"
                     /locus_tag="P5123_03825"
     CDS             complement(7258..7629)
                     /gene="queD"
                     /locus_tag="P5123_03825"
                     /EC_number="4.1.2.50"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020268275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0008616 - queuosine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6-carboxytetrahydropterin synthase QueD"
                     /protein_id="HRX15420.1"
                     /translation="MFFLTVEDHFSSAHQLRGYKGKCENLHGHNWKVLLTVKGEILNE
                     IGLLIDFHDLRKMLKETLALIDHCNINETVHFKSTNPSSEELAKFIYLQMESLLEKEK
                     TVSVKTVTVFESEKCSCSYEK"
     gene            complement(7649..8680)
                     /locus_tag="P5123_03830"
     CDS             complement(7649..8680)
                     /locus_tag="P5123_03830"
                     /inference="COORDINATES: protein motif:HMM:NF013227.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DegT/DnrJ/EryC1/StrS aminotransferase family
                     protein"
                     /protein_id="HRX15421.1"
                     /translation="MSIVSNKPTITRKELEGVLDCLINDELTSGEAVKIFEKALCELT
                     GYKNSVAINSATSAYHLAFTALGIEEGDEIILPSYFSSHALNAIKICRAIPVLIDIDD
                     NSLSASPDKYAIKINDKTKAIIMGHTGGIYIDSSSFKELTVPVIEDISHCIGIESKEG
                     NEGYFGSIAVCSFSPNDMITTGNGAAVFTNNTRFYSTIREKRYNEETTHFDYSMTDFQ
                     AAMGISQISRIQDFIRRRIEIAKIYYDRVRLTQHSVLLPFSSDYIYQSFPVIFDATYD
                     KIVKFFKKNGIELYSPVPKPLHSYLNLKGMDYPNSDRMCKKMFSLPIYPTLTKNEIEK
                     IGRTVAKFI"
     gene            8954..9127
                     /locus_tag="P5123_03835"
     CDS             8954..9127
                     /locus_tag="P5123_03835"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRX15422.1"
                     /translation="MVTLKITMFQWLHLLSVCFKDNFRVTDEVFILGYKLIVLLRITS
                     KDDFLVTRLIIGG"
     gene            9184..10023
                     /locus_tag="P5123_03840"
     CDS             9184..10023
                     /locus_tag="P5123_03840"
                     /inference="COORDINATES: protein motif:HMM:TIGR00413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="septal ring lytic transglycosylase RlpA family
                     protein"
                     /protein_id="HRX15423.1"
                     /translation="MRVYGVLSKVFLVIIMLVFSAGCAQSKNMTRNDTVVEDMPYDSD
                     SEDVSFSGDGLDDIGSGDGGLDDGYITAAGAGGGGADFDQTGNASWYGRAFHGRKTAS
                     GESFDMYEMSAAHKSYPFGTVLEVKNLDNGKSVQVRVNDRGPYVDGRIIDVSYAAGRD
                     LGMLTTGTARVGLKVVSSPGAAPVSGGVPIDDSYGYGSPDVGAPVSTGAYSLQCGAFY
                     SKKNADNLRDKLSGMFENRIEVIHEDDLYKVRILDIPSKEEANRFKSILSGEKIPGYL
                     IKN"
     gene            10229..10301
                     /locus_tag="P5123_03845"
     tRNA            10229..10301
                     /locus_tag="P5123_03845"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:10262..10264,aa:Thr,seq:ggt)
     gene            10481..10633
                     /gene="rpmG"
                     /locus_tag="P5123_03850"
     CDS             10481..10633
                     /gene="rpmG"
                     /locus_tag="P5123_03850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009179755.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L33"
                     /protein_id="HRX15424.1"
                     /translation="MRQIVLLQCSECKRKNYATMKNKRNTQGKLQLKKYCKFDRRHTE
                     HKETKA"
     gene            10643..10825
                     /gene="secE"
                     /locus_tag="P5123_03855"
     CDS             10643..10825
                     /gene="secE"
                     /locus_tag="P5123_03855"
                     /inference="COORDINATES: protein motif:HMM:TIGR00964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecE"
                     /protein_id="HRX15425.1"
                     /translation="MKQFIEYLKGSREEMKKISWPNREEVSRFTFVTIVTVFIVAMIL
                     WVMDTVLMQLISLVMK"
     gene            10822..11373
                     /gene="nusG"
                     /locus_tag="P5123_03860"
     CDS             10822..11373
                     /gene="nusG"
                     /locus_tag="P5123_03860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011937986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006353 - DNA-templated transcription
                     termination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination/antitermination
                     protein NusG"
                     /protein_id="HRX15426.1"
                     /translation="MMEMKWYVIHTYSGHENKVKIAVENKIRNLGLEERIGEIRIPTY
                     DDPYLDKKGKKRTRSKKTLPGYILINMDLDNETWGIVKSTPGVTNFVGGFGDGKPKPL
                     REDEVGALLFEGSTSKATKERATVGIDFSVGEHVKVIEGPFNTFTGVIEEIFPEKGRL
                     RIKVEIFGRGTPVELDFIQVNKI"
     gene            11417..11851
                     /gene="rplK"
                     /locus_tag="P5123_03865"
     CDS             11417..11851
                     /gene="rplK"
                     /locus_tag="P5123_03865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015746337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L11"
                     /protein_id="HRX15427.1"
                     /translation="MAPKKKKLATQIKLQIPGGQANPAPPVGPALGQHGLNIMDFCKA
                     FNDRSKDQMGTILPVVISVYEDRSYDFIIKTPPAAVLIKKVLKLQKGSAEPNKTKVAT
                     ITRAQLEEIAEMKKPDLNSNDMDAACNIIAGTARSMGVDVSN"
     gene            11936..12619
                     /gene="rplA"
                     /locus_tag="P5123_03870"
     CDS             11936..12619
                     /gene="rplA"
                     /locus_tag="P5123_03870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011345204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L1"
                     /protein_id="HRX15428.1"
                     /translation="MKRGKKISSARQKVDKTALLTPEDAIALMKELKFANFDESVEIA
                     VKVVHKSYQNIRGFVTLPAGNGKDVRVLVLCTGDKQDEAKAAGADFVGADDLIEKIQG
                     GWTDFDAVVATPDLMPKVGKLGPVLGRKGLMPKPKAGTVTTDVKGIIGELKSGKLEYR
                     SDKTGVIHLGIGKCSFEDSKIASNIVAVYQQIMRDKPSDAKGTYVRSLCISSTMGPGI
                     KINHKGIDK"
     gene            12619..13137
                     /gene="rplJ"
                     /locus_tag="P5123_03875"
     CDS             12619..13137
                     /gene="rplJ"
                     /locus_tag="P5123_03875"
                     /inference="COORDINATES: protein motif:HMM:NF000955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L10"
                     /protein_id="HRX15429.1"
                     /translation="MVKQYKVDFVNEIKDALEENKNFILTKYSGIKVSELTALRSQIR
                     EKGATYKVVKNNLFKRALDDKGYSSIEEHLKGPIGVAFAGDQIGDVAQVLKAFKKEQK
                     LFEYFLGVIEGTVYDSAALAKIADLPSKEVLLSQIMSLINGPATGIAVGSKEVMNSLA
                     RAINAVAEKNNA"
     gene            13265..13645
                     /gene="rplL"
                     /locus_tag="P5123_03880"
     CDS             13265..13645
                     /gene="rplL"
                     /locus_tag="P5123_03880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018214412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L7/L12"
                     /protein_id="HRX15430.1"
                     /translation="MAKLTTEELLDAIGGLTLLEASELVSAMEEKFGISAAAPVAVAA
                     APAAGGGADAAEEKSAFDVVLTNFGANKIGVIKEVRAITGLGLKEAKALVEEGGAVKE
                     GVSKDEAADIKSKLEAAGATVEVK"
     gene            13823..>16580
                     /gene="rpoB"
                     /locus_tag="P5123_03885"
     CDS             13823..>16580
                     /gene="rpoB"
                     /locus_tag="P5123_03885"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017214121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000345 - cytosolic DNA-directed RNA
                     polymerase complex [Evidence IEA];
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta"
                     /protein_id="HRX15431.1"
                     /translation="MQKTKKVVNFGKINPNVELPNLVEVQLNSFEWFLQAGLPQKKRH
                     YQGLNSVFADIFPIESPHEDLVLEFIDYEVGDPKYSEAECKERDVTFASPLKSTIRLI
                     KKDSMEVREQSVYMGDIPLMTERGTFIINGAERVVVNQLHRSPGIFFAFDDANRVYSS
                     RVIPDKGSWLEIEMDSKGFIIARIDRKKKFPVTILVKALGYETNEDIIKLFYPVEEIK
                     LKDEESYEAALGRRVASDVVSGETGEVIIEAGNRINIDTVDIFKEEKIKSVELISFPN
                     NKDDMYLVTTLQKDADLLAKQNVVDAEELSQSQLALLTIHELMRPGEPSTLENAENEF
                     QRLFFNPKTYTLGNVGRYKINKKFGYEDDDLSSTLTKRDILETVKYVFYLIAEADGFI
                     VDDIDHLGNRRVRSVGELLTNQIKVGFQRMERVIKERMTIQDLDLVTPQALISIKPIS
                     AVINEFFGSSQLSQFMDQTNPLSELTHKRRLNALGPGGLSRDRAGFEVRDVHHTHYGR
                     MCPIETPEGPNIGLILSMSTYSRINDYGFLETPYKIVKDGTVTSSVKYLTATEEDRYN
                     VAQANASIDDNGVFTENLIPCRYRGDFPYRKPEEIQYMDVSPQQIFSVSTCLIPFLEH
                     DDANRALMGSNMQRQAVPLMVEEAPLVGTGIETYAAMDSGVMIRAKNAGDVEYADANI
                     VKVRRKDNGELDIYPLVKFKRTNQGTCFNQKPAVRVGDKVKKGGIIADGPSTDNGRLA
                     LGKNILVAFMPWMGYNFEDAILLSERLVKDDVYTSLHIEQFEVEARETKLGPETITRD
                     IPNLSEKAFKDLDADGVVRPGAYVKPNDILVGKVTPKGEQDITPEYKLLHSIFGEKAR
                     EVRDTSLRVPNGMEGVVIDIKRFSRENGDELSAGVEELVKVFIAKKRKISVGDKMAGR
                     HGNKG"
BASE COUNT         4942 a         2884 c         3604 g         5150 t
ORIGIN      
        1 ctaaaggaat ttttcgcaag ttccttgttg aaaagttaat ttttagaggt tgccatcagg
       61 gagtgctatt tgtatgcttg aaaagttaaa ggctcttgag agccgtttta aaaaaattga
      121 acaggagatg agtgatcctg atataataaa ggatcagacc cgcttcaggg acttaaaccg
      181 agaacattcc agattatcac ctgtaattga atcgttcgct gaatataaaa aggctgtatc
      241 tgaactagcc gacactaaaa aaatgctcga agacgagcgt gatagtgata tgcgagaaat
      301 gcttgctgca gaaaagcgtg atcttgaaga gaaaatccca cgaatggaac aggatctcac
      361 cattatgctt ctacctaagg acccacattc tggtaaaaac gtaataattg aaatccgggc
      421 aggtaccgga ggtgaagagg ctgctctgtt tgtctctgat ctttatagaa tgtacacccg
      481 ttacgccgat ctaaaaaaat ggaagatcga aatcatggat cagaatcaga cagaaattgg
      541 tggattaaaa gaggtaactt tccttgtttc tggcgcagat gtatacgatg aattgaaata
      601 tgagtctgga gctcacagag tgcagcgggt tcctgaaaca gaatccggtg gacgaatcca
      661 cacttctgct gtaaccgtag ctgttatgcc ggaagccgaa gacacagatg tcgaaatacg
      721 tcaggaagac cttagagtgg atgtttatcg cgcttccgga catggggggc agtctgtaaa
      781 tacgaccgat tccgcagtac gaataaccca tatcccaact ggccttatag tgacatgcca
      841 agatgaaaaa tcacaattaa agaacaagca aaaagccatg aaggttttgc gagcacgact
      901 cttcgagctt gaagagcagc gacgccaaag tgaacaatcg caaatgcgta agtcgcaagt
      961 cggttcgggc gaccggagcg agcgaatacg tacatataat tttccacagt cgcgtcttac
     1021 ggatcaccgt ataggcttaa cgctctattc actggaatca gtattggaag gagaacttga
     1081 accggttatt gttcctttaa aagaatatga aatagaacag cttttaaaaa gtaatgatac
     1141 tgtatgacaa ttaattctct gatttcgcaa tgcgccaatc gtttaagaga cgctggtatt
     1201 gcaagttatc aacttgacgc gcgaataatt atagcatatg tgttagatat aacttccgag
     1261 aagttgcttg cggagggaaa ttccttggtt gctgaagatc atgttgctcg gatatgcagt
     1321 ttatgtgatc gtcgtcaaag aagagaaccg cttgcttata ttactggaat aaaagatttc
     1381 tattctttaa aatttatggt aacaccggat gtgcttgttc caaggcctga aacagaactt
     1441 ctagtagaat atctgattga taactgtcca aagaacagta ctgttcttga cctttgtacg
     1501 ggaagcggag caattgctgt aacattaaag tatgagcgtc ctgatctgac ggttacgggt
     1561 tccgacattt ctgtttcagc actcgaggta gccagaaaaa actgtaaatt caattctgtt
     1621 gacaaaatca actttataga gtctgatctc tttgaaggta taaccaataa atatgatgta
     1681 attgtttcaa atcctcctta tattccgtta tcgtgtaaag agtcactcga accggaactg
     1741 ggatacgagc cgcagattgc ccttttttcg ggtgaagatg gcatggattt gccaacaaag
     1801 atcattaatc aagccagaga ttatcttttc agcaatggct ttctggttat ggagatagac
     1861 agtcggtcag gagaaaaaat tatcctggca gcaaaagaaa agaatatggg ttgtgagtta
     1921 atgtcggatc ttgcgggtta tgacagaatt gcagtaataa ggatatgatt cagacagatt
     1981 attaacagga acagttattt tatggaatac tcattatctg acggaactag gcctcgtttt
     2041 cttgtcataa agtacatatt gagggtgatg gctccctata tatttcttct gtttttcctt
     2101 attgcaatta atttcggttt caatatttac tcttcttggg gcggagtaaa cctggttgta
     2161 aaactataca tatatacttt tgcatttttt cttgtttata ctatttcaat atcgttgccg
     2221 cctgaattgc gttccgccta tagtcgctat ggtctgctta caaagttcat ctatgtgctt
     2281 gaaagccgta tttttccttt cgcacttatt tatattgtta cagccatcta ttctgttgtc
     2341 gagaacatgg gtagccaata ctggccttat gactcatttg ccggaatctt cgatgatgcg
     2401 atatcaaacc tggtgatcta ttcggttgta ttgatgtatg tgctaaaaat taaaaaacgg
     2461 ccatacgtga ctgttccggt atatcttttc cttatatatc tttattatat gattaaccag
     2521 cggttttatc agaactttta tgctggtaac gcaatggtag cttttaaaat acttaaggct
     2581 gctctactct tcttcttgat gacctggagt acaaagctca aggcatcacg tttttttctc
     2641 aacctttgtt tttctctact tttagctatt gctttgaacg cattgattat tggtatatac
     2701 aagcttagct acaataatgt ttcgaaccgg gtactaaaaa acagtattgc cttgaaactg
     2761 acaaagtttg gtcgcccatc ggctctttct cctcttatga aagatgtgct tgataagaaa
     2821 gatattaccc ttctggatca aatatatccc tatgttaagt attatggaaa aaactttgat
     2881 ataagtgaaa tacagtggag agagtttcta tttcacgatg atgtatcttt tgccaaccga
     2941 gtggcttcat atatgcttaa agagggcgtt tctgctcatc ctgccgaaat aattgatttt
     3001 gttaaaaaaa atattaaaca agaccctgac agtattcgta attccgagtt tttaatacgg
     3061 ttaactgctg tttgcttaaa tgagtatggt tctaataagg agcttgttga agctatgcat
     3121 atgtctgatg atgtattcct gatatgggca ataaaagtga tgggtgaaac tggcgattgt
     3181 catcttattt cagagattgt ggtttatcta aatcatattg attcagatat ttctgatgct
     3241 gcgtataatg cactaaaact tctaactgga atagatccct atgctcagag tcagattcct
     3301 tataattcag tcagaagtct aaagctcttt aatgattact tgcataaaag ttgcaaatag
     3361 aatatgcaac acaaattgaa cagtcaggtt ttctagcgaa gcatgttttg cgaccatgat
     3421 taatcattaa caggtgaaac tccagccatt tttccggggc aagcatcgac ttcagctctt
     3481 tctctattat atcaggattt ttggatctcg taagaccgat tcttcttgct attcgaccca
     3541 catgtgtgtc aacagcaagg ccgggttttc caaatccttg tgaaattaca acattcgcgg
     3601 tttttcttcc tattcctgga agttttatga gctcttctat ggaatctggt aaaataaagc
     3661 ttttttcact taaagccttt gcaaggccaa gtatgttctt cgccttgctg tggtaaaaac
     3721 cagtgctatg tacaattttt tctatttcgt ctagtgaagc attgcttaaa gatttaaaat
     3781 cggggtattt taaaaaaagc tcgggagtta ccttgttaac ctgtttgtct gttgtctggg
     3841 ccgataagac taccgcaatg gttaattcgt aaaggttgct ataggtaagc tcgggatttg
     3901 tttcgccata cttttttttt agcagtttgt acagcatgtc agcttttgat tccattatat
     3961 ttgaaccata gttttaataa attctaaaaa tttttgtaat gcaagagctc tgtggctaat
     4021 acggtttttg gtttccggtg caacattggc cattgtagtc ttgtattcag aaacataaaa
     4081 aatgggatca tatccaaaac cattctcgcc gattattttc ttgctaatca tgccttcaca
     4141 tgtgccttca aaaaaatggg tattgcgttt gctgtcaata aatgctaata aacaaacaaa
     4201 tctggcggtt ctctctttct ccgctacgtc tttcagtaat tcaagtataa gtctgttttt
     4261 ctcatcatca gattcaagcc cgtggtatct tgctgaaaaa attccgggtt ctccgtttaa
     4321 tgcatctata acaagaccgg aatcgtcggc aattatcggt ttatccgtat attctgttag
     4381 agcttttgcc ttaattagtg cgttttcttt gaaggtattt ccgtcttcaa ttatatcttt
     4441 cggataatta ttcaaacgag acatagcggt aaagttacaa attgcggatg ctccggcaat
     4501 attattcatc tcatctatct tgtgtgagtt agtgctggcg gtgattattt ctatcatttc
     4561 atttgcctta aatagtattt cttagttatc ttgatacccc tcttttccca ttcagtaaat
     4621 ttcatgtcaa tttttaaaat ttctttttca aaatagtgaa tttagaataa tttaaataca
     4681 tttctagtta tgtgcttcaa aattcgtcta aacgaatata ttttctataa acggtggttt
     4741 ttacaaaata ctctttgaaa ataagttgaa aaaaaaaggc aatcagttac attctagtta
     4801 tgttaattaa aaagattctt cttaatctat cattatatat tcctgatgta gcccttattc
     4861 tgtttttgac agaggagggt atatttgtgc ccgaagaacg aagccatttt attgtggttc
     4921 tcgtctattt tctgataaaa attagcctgc ataatattgt cgcttttttt tgttttatca
     4981 aaccttattc tgcggtaaaa gatatactgc ttcaattcca acgagggcgt tacagcatgg
     5041 cggatagtgc gcatgtttta aaaccttctg cctgctttgg agatctgttc gaactgctcc
     5101 gttctacagg aaggtaccta gataatctgg tcggttctca aaatgatgag atcgaaaagt
     5161 tcagagaact gtataataat gtgatattat cagcaaacag ttattttgta gtgcttaacg
     5221 ctcgtgatga agtgattttt gctaacaaaa gcttttcggc gaactttcga tataccgatg
     5281 atggaatatt ggggctcaga ctcgaagata tattttacat tatacctgga cgattctctg
     5341 atgcgataga taatgtaaaa aaaagaggtc agtccgcggt attgcgaaag atcaagctga
     5401 tttcgcggcg aaagatatca ctcatagctg atataaaaat atcgcgggtt actgttcagg
     5461 gagagaagca atatattgta gttatggatg atattacttc ccaatggcgc aaagattatc
     5521 aaatctcgct tattagccaa atatccgagt cacttcaacg tgatgaagaa gtagaacgaa
     5581 tcctttatgc tatattgacc gccgttactt ctggttccgg acttggtttt aaccgagcaa
     5641 tgttattctt atataatgaa cagaaaaaaa ttctaaccgg caagatggct gttggtccag
     5701 attcaatgga agaagccgta caaatatggg gttctgttca aaacgaagag ctaaatcttc
     5761 ttggcggtgg gcctgatgct ccgcctgttc tccaaacaaa tggagccttt tacaagcagg
     5821 ttgtaacaag taattttgca attaatggaa atacactttt tgccgatgtt tttaataaac
     5881 gtgctccggt tcatatatac gacgcggcaa gtgatcctcg tgtggatccc gaaacacgaa
     5941 agtttatgga tgtttcagag tttgttgtag tgcctctgtg ctcggccaat aaggctcttg
     6001 gagtcgttgt ggcagataac aagtttaatg gtgcaccaat atatgaggac catgtcgagc
     6061 ttctctctat ttttgctgtt caggcttctt tatcaataga cagttacaat tctttaactt
     6121 ctgttaaaga acgaatggag catattcagg ctaagcagga ggctattgta gagagcgaaa
     6181 agatggcagc tgttgggcga atagccgcgc atattgccca cgaaataaga aaccctcttg
     6241 tgactgttgg tggctatgca agacgcatat tgcagtccgt aaaaaaaccg caaaaaactt
     6301 tggaagatat cgaaaaagct gcttcgataa ttcttcacga atccgagcgc ctggaaaaaa
     6361 tactctctaa tgttatggat ttttcccgtc cgtcacctct gattcgtgaa tataataatc
     6421 ttaacgacgt tgttaaggat acctattatc tgttaaaaaa tgtattacaa gaacgaaaaa
     6481 taacagctac gcttgatttg gaacagaaac ttccgttggt aaagtcagat tttaatcagt
     6541 taaaacaggt tgtgcttaat ttgttacaaa atgccatgga tgccacacct gccggaggct
     6601 ccattatact taaaacagga ctgacggaaa cacatattta tatacgaatc aaggatagtg
     6661 gtactggaat cagtcccgag gctatggaca aaatatttga tccttttttt acttccaaaa
     6721 taactggcgt aggccttgga cttgctgtgg ttaagaaaat agttaatgat cataacggtg
     6781 aaatagtaat aagcaataat tctgaaggcg gttgtcaatt ttcagttgaa ttattacgcc
     6841 cttcataata tagtaatact tataacaaag agatgacgga ggagtcatga gcagaatatt
     6901 gttggtagaa gatgaaaaaa atcagagaga gttatatgct gccgaattac gtgatgatgg
     6961 ttacgaagta gaagaggctt ctgatggcaa aatggcagtt gaaaaggtta aatcaagcaa
     7021 atatgatctt gtagtgcttg acatccgtat gccagagatg gacggaatcg aggctcttgg
     7081 aaagatactg tcgcgtgata aaaaaatccc tattgttata catacagctt attcaaacta
     7141 taaaagcaat tttatgacat ggacagcgga tgcctatgtg acaaaatcat ccaatcttga
     7201 cgagttaaaa actaagattg cggagattct tgccgcgcga ggtcaggctt aggctaatta
     7261 tttttcatag ctacagctgc atttttccga ttcaaaaact gtaacagttt ttactgatac
     7321 agttttttct ttttcaagca gagattccat ctgcagatat atgaattttg ccaactcttc
     7381 cgaactgggg tttgtgcttt tgaagtgcac agtttcgttt atgttgcagt ggtcaataag
     7441 ggcgagtgtt tcctttagca ttttgcgcag atcatgaaag tctataagaa gacctatctc
     7501 gttaagaatt tcacccttaa cggttagcaa tactttccaa ttgtggccgt gcaggttttc
     7561 acatttgcct ttatagcctc gaagctggtg cgctgacgag aagtgatcct caactgttaa
     7621 aaaaaacatg gtttctctga tcctttttct atatgaattt agctacagtg cgaccgattt
     7681 tttcaatttc attttttgta agggttgggt aaatcggtaa cgaaaacatc ttcttgcaca
     7741 ttctgtccga gtttggataa tccattcctt taaggtttaa atagctgtga agaggtttcg
     7801 ggactggtga atagagttca atgccgtttt tcttgaaaaa tttcactatt ttgtcataag
     7861 tagcatcaaa aataaccgga aatgattgat aaatatagtc actactgaag ggcaacagaa
     7921 ctgaatgttg agtcaaccga accctgtcgt aataaatttt ggctatttct attcttcttc
     7981 ttatgaagtc ctgaatacgg ctaatttgtg atatgcccat cgctgcctgg aaatcggtca
     8041 ttgaatagtc aaaatgagta gtttcttcat tatatctctt ttctcgtata gttgagtaaa
     8101 aacgtgtgtt attggtaaaa acggcagcac cgttacctgt agttatcata tcatttggag
     8161 aaaatgaaca aacagcaatt gaaccgaagt atccttcatt tccttctttt gattcaatac
     8221 ctatacagtg tgaaatgtct tcaattacag gcactgtaag ctctttaaaa gaagatgagt
     8281 ctatgtatat tcctccggtg tgacccataa taatcgcttt tgtcttgtcg ttaatcttta
     8341 tagcgtattt atcgggggag gccgacaggg agttatcgtc aatatcaata agaaccggta
     8401 tggctcggca tatcttaatg gcgtttaagg cgtgtgatga aaagtatgaa ggtagtatta
     8461 tttcgtcacc ctcttctatg cccagagcag taaaagccag gtggtaggcc gaggttgccg
     8521 agttaattgc gacactattc ttgtatccgg ttaattcgca aagagctttc tcgaaaatct
     8581 tgaccgcttc tcccgaggtg agctcgtcgt ttataagaca atcgagtacc ccctctagct
     8641 ccttgcgcgt aattgttggc ttatttgata caatactcat atagttctcc tttagtaaag
     8701 aaggcagtat agctgataat tttaaatatg tttatagaaa tttctctagg ttagtacaaa
     8761 tgaatggcgg ttaccctgtg gtcaagaacg tttttcttaa cctctgaggc aaacatgaaa
     8821 cgagtatttg tataaaagaa aacgataatt gtctcagctt ggtgtttttt atgtgattgg
     8881 tctctttatt ggattatgtg tattaaagct attttgtaaa acgtccgatt attatggagt
     8941 gtgttgtttt attttggtaa ctttgaaaat cactatgttt cagtggcttc atcttttaag
     9001 tgtctgtttt aaagataatt ttcgtgttac cgatgaagtt tttattttag gctataagct
     9061 tattgttctg ctaagaataa ccagcaagga tgattttctt gtaacacggt taattattgg
     9121 tggttgactt ttgtttgtta tttaccgcgg cctgatgccg ctattgattt acgggaggtt
     9181 cgcatgagag tatatggagt cttaagcaaa gtttttttag tgataatcat gcttgttttt
     9241 tcagcaggtt gtgcgcagag caagaatatg acacggaatg acactgtcgt cgaggatatg
     9301 ccttatgata gtgatagtga agatgtttca ttctcgggtg acggcttgga tgatatcggt
     9361 tcgggtgatg gtggtctgga cgacgggtat ataactgctg ctggagctgg tggcggtgga
     9421 gccgatttcg atcagactgg caatgcctcg tggtatggcc gggcttttca tggccgcaag
     9481 actgcttctg gtgagtcttt cgacatgtat gaaatgtctg ccgctcacaa aagctatcct
     9541 tttggtactg tgcttgaggt taaaaacctt gataatggta aaagtgttca ggttcgagtc
     9601 aatgaccgcg gaccatatgt tgatggcaga ataattgatg tctcatacgc agctggacgc
     9661 gatctgggta tgcttacaac gggaactgcc agagtggggc taaaggttgt gtcgtcacct
     9721 ggcgctgcac cggtatcagg cggtgtgccc atcgatgatt cttatggata tggctcaccc
     9781 gatgtaggag cgccggtttc aacaggtgcg tattctttac agtgtggggc cttttattcg
     9841 aaaaaaaatg ccgataattt gagagataaa ctttctggaa tgtttgaaaa ccgtattgag
     9901 gttatacatg aagatgatct ttataaagta cggatacttg atattccttc taaagaagag
     9961 gctaataggt tcaagagtat tctttccggg gagaaaatcc caggttatct gattaagaac
    10021 taaaattaag tttacataat cacaatttat acgattcaaa attttgatta ataacaggaa
    10081 gtctgttctt cttacagact tccttcgttc tttccattct ctaaaatcat accgaaaaca
    10141 ccatttttaa aaaaaagtct tgatattccg cattaattaa aaaaatattg acagaaaatg
    10201 gcactattgg ctatatcgga cagactatgc ccgcgtagct cagtaggtag agcacatcca
    10261 tggtaaggat ggggtcacca gttcgaatct ggtcgtgggc tcggtagttt gctggttatc
    10321 ctgcgaaaaa tgataaaggc gcttccaaaa acatcattaa actataaagg tttctgtgtg
    10381 ctttctcttg gctttacggg tatgcagttg tttttccctt tttctgttcg gttgatttgt
    10441 tttggggttg tcttaaatac taacatacgg aaggcgtatt atgagacaga ttgttttact
    10501 ccagtgttca gaatgtaagc gtaaaaacta cgctacaatg aaaaacaagc gtaacactca
    10561 gggcaagttg cagctgaaaa aatattgcaa gtttgatcgt cgtcatacag agcataaaga
    10621 gaccaaagca tagggagata tcgtgaaaca gtttattgaa tatctaaagg gttcgcgtga
    10681 agagatgaag aaaatctcat ggccgaaccg ggaagaggtt tcccgattta cctttgttac
    10741 cattgttacg gtgtttattg tagccatgat tctttgggtt atggatacgg ttctaatgca
    10801 gctaataagt ctggtgatga aataatggaa atgaagtggt atgtaataca tacatactcc
    10861 ggtcacgaga acaaggtaaa gattgctgtc gaaaacaaga ttcgtaatct gggtctcgaa
    10921 gagagaatcg gtgagataag aataccaacg tatgacgatc cctatcttga caagaaaggt
    10981 aaaaagcgta ctcgttccaa aaagactctt ccgggttaca ttctgatcaa tatggatctg
    11041 gataatgaga cttgggggat agttaagagt actccgggtg taacaaattt tgtaggtggt
    11101 tttggcgatg gcaaacccaa gccgcttcgt gaggacgaag ttggcgctct tcttttcgag
    11161 ggaagtactt cgaaagcaac taaagagcgc gcgactgtag gtattgattt ttcagttgga
    11221 gagcatgtga aggtaattga aggtccgttt aatactttta caggcgttat tgaggagatt
    11281 tttccggaaa aaggcaggtt gcgtatcaaa gttgaaatat ttggacgtgg aactcctgtg
    11341 gaacttgatt ttattcaggt aaataaaata taatcattaa tttgatgaat tacattcgta
    11401 tagaaaggaa tataaaatgg caccgaaaaa aaagaaacta gcaactcaga taaaacttca
    11461 aattcctgga ggtcaggcta atccggctcc tccggttggt ccagcgcttg gtcagcatgg
    11521 tcttaatatt atggatttct gtaaggcttt taatgatcga agtaaggatc agatgggaac
    11581 tatacttcca gtcgtaataa gtgtttatga agacagaagt tatgatttca ttataaagac
    11641 tccgcctgcc gcggttctta ttaaaaaggt tctgaaactt caaaagggtt ctgccgagcc
    11701 gaataagacc aaggttgcaa ctattacccg agctcagctt gaagagattg cagaaatgaa
    11761 aaaaccggat ttaaattcaa acgatatgga tgccgcatgt aatattattg cgggtacagc
    11821 ccggtctatg ggtgttgatg tttcaaactg atttaaaagt tttggttaat aatggatttt
    11881 gttgtcattt tgaaacgaca gcgaatgtcc taaaaataca agcaatgagg atacaatgaa
    11941 gcgaggaaaa aaaatcagca gtgcgagaca gaaagtcgac aaaaccgctc ttcttactcc
    12001 tgaagacgcg atagcactga tgaaggaact caagttcgct aattttgatg agtctgtaga
    12061 gattgctgtc aaggttgtac acaaaagtta tcagaatatt cgaggatttg ttacacttcc
    12121 tgcgggtaac ggtaaagatg ttcgtgtact tgttctttgt acaggcgata agcaggacga
    12181 ggcaaaagcg gccggagcag actttgtcgg agccgatgat ctgatcgaaa aaattcaggg
    12241 cggttggaca gattttgatg cggtagttgc tactcctgat ctgatgccta aagttggaaa
    12301 gcttggacca gttcttggta gaaagggatt aatgccaaaa cctaaggcgg gtacagttac
    12361 aactgatgta aagggaataa tcggagagct taaaagtggt aagttggaat atcgttctga
    12421 taaaacgggc gttatccatc ttggtatagg aaaatgctct tttgaagatt caaaaatcgc
    12481 atcaaatatt gtggctgttt atcagcagat tatgagagat aaaccatcag atgccaaagg
    12541 aacttatgtc cgatctttgt gtatctcttc aaccatgggt cctggtatta aaatcaatca
    12601 taaggggatc gataagtaat ggtaaaacaa tataaagtag atttcgttaa cgagataaag
    12661 gatgctctag aagagaataa gaactttatc cttacaaaat actccggtat caaagtttca
    12721 gagcttactg ctcttcgctc acaaatccgg gaaaagggcg ccacatataa agttgtcaaa
    12781 aacaatcttt ttaagcgagc tcttgatgat aaaggctact cctcaatcga agaacatctg
    12841 aaaggaccta tcggtgttgc ttttgccggt gaccagatag gtgatgttgc acaggttctt
    12901 aaagctttta aaaaagaaca gaagcttttc gaatatttcc tgggagtaat tgagggcact
    12961 gtatatgaca gtgcagcgct agcaaaaata gctgatcttc cttcaaaaga agttctgctt
    13021 tctcaaatca tgtctcttat aaatggtcca gctaccggta tcgcggttgg ttcaaaagag
    13081 gttatgaatt ctctggctcg cgcgataaat gcggtagctg agaaaaataa cgcgtaatta
    13141 tgttcattac ataatatagg gtcgaaaagg ttggttttta attaagctga ttttcagccc
    13201 cttataggtt tcacacagta agtgtgttaa cttgaagaaa ttcactaagt ataaggagtt
    13261 tacaatggct aaacttacaa cagaagaatt gcttgatgca attggcggtc ttacacttct
    13321 agaggcatcg gaacttgttt ctgctatgga agaaaaattc ggtatctctg ctgctgctcc
    13381 agttgcggtt gctgctgctc ctgctgcagg tggcggtgct gatgctgcgg aagagaaatc
    13441 cgcgtttgat gtcgtactta ctaactttgg tgcaaacaaa atcggtgtta tcaaggaagt
    13501 tcgtgctatt actggtcttg gacttaaaga agcgaaagct cttgtagaag agggtggagc
    13561 tgttaaagaa ggcgtttcta aagatgaagc tgctgacatt aagtcaaaac ttgaagctgc
    13621 gggtgcgact gtagaagtaa aatagttcca ttttgcggac aatgttcgca aaatggggtt
    13681 tataaaattc gtaatatgat acaatttgca atcttgcaat tgtaaataga cggaattttg
    13741 tactcctaaa aaataggttg ggttgattgt atcaatccga cctattttgc tctttcaata
    13801 ctaaaatctc ttgaggtcaa aaatgcaaaa aaccaaaaag gttgtcaatt ttggtaagat
    13861 aaatccaaat gttgaactgc caaaccttgt tgaagtccag ctgaattcat ttgaatggtt
    13921 tttacaggcc ggactgcctc aaaaaaaacg acattatcaa ggacttaatt cagtatttgc
    13981 cgatattttt ccgattgaaa gcccgcatga agatcttgta ctggaattta ttgattatga
    14041 agtcggtgat ccgaaatatt cggaggctga gtgtaaagaa cgcgatgtta cttttgcctc
    14101 tcctcttaaa tctacaataa gattaataaa aaaagacagc atggaggttc gcgaacagtc
    14161 tgtttatatg ggcgatatac cgcttatgac tgaacgcgga acttttataa taaacggtgc
    14221 ggagagggta gtcgtaaatc aacttcaccg ctctccgggt atattttttg ctttcgatga
    14281 tgcgaaccgt gtttatagct cgcgtgttat acctgataaa ggttcttggc ttgaaataga
    14341 gatggattca aaagggttta ttattgcccg tattgaccgt aaaaaaaagt ttcccgttac
    14401 aatattagtt aaggctctcg gctatgagac caatgaggat attatcaagt tattttatcc
    14461 tgttgaagag atcaagctta aagatgaaga gtcttatgaa gctgcgcttg gtcgtcgtgt
    14521 agcgtctgat gtagtaagtg gcgaaactgg tgaggttatc attgaagctg gtaacagaat
    14581 aaatatcgat accgttgata tttttaaaga agaaaagata aaaagtgtag agttaataag
    14641 ttttccaaac aacaaagatg atatgtatct tgttacaact ctacaaaagg atgcggatct
    14701 tcttgcaaaa cagaatgttg tcgatgctga agagctctct caaagtcagc ttgctctact
    14761 cacaatacat gaacttatgc gtcctggtga gccttctaca cttgaaaatg ccgaaaacga
    14821 attccaaagg ttgttcttta accctaagac ttatactctt gggaatgtcg gtcgttacaa
    14881 aattaataaa aagtttgggt atgaggatga tgatctctca tcaactctaa ccaaacgtga
    14941 tatactggaa accgttaaat atgtttttta tcttattgct gaggcggatg gttttatcgt
    15001 ggatgatatt gaccaccttg gaaaccgtcg cgtcagatct gtaggtgagc ttttaacaaa
    15061 ccagattaaa gttggttttc agagaatgga gcgtgttatt aaggaaagaa tgacgataca
    15121 ggatcttgat cttgttacgc cacaggctct tattagtatt aaacctataa gtgcagtaat
    15181 aaatgagttc tttggctcca gtcagctttc gcaatttatg gatcagacaa atccattgtc
    15241 tgaactgact cataaacgaa gacttaatgc gcttggtcct ggaggtttgt caagagatcg
    15301 tgccggtttc gaggttcgcg atgttcacca tactcattat ggccgaatgt gtcccattga
    15361 aactcctgag ggtccaaata ttggactgat actatcaatg agtacatact caaggataaa
    15421 tgattacggt tttctagaga ctccttacaa gatagttaag gacggtactg tcacttcatc
    15481 tgttaagtat ctgacagcaa cagaagagga cagatacaat gtagcacagg ctaacgcttc
    15541 aatagatgac aatggtgtct tcactgaaaa tcttatacct tgtcgttaca gaggcgattt
    15601 cccttatcga aaacctgaag agattcagta tatggacgtg tctccccaac agatcttttc
    15661 ggtttcaaca tgtcttattc cttttcttga gcatgatgat gccaatcgcg cgcttatggg
    15721 ttcaaacatg caacgtcaag cagttccgct aatggtagaa gaggcgcctt tggtaggtac
    15781 cggtatagag acctatgcgg caatggattc aggcgtaatg attcgtgcca aaaatgctgg
    15841 tgatgttgag tatgctgatg cgaacatcgt taaagtgcga agaaaggata atggtgagct
    15901 tgatatttac ccacttgtaa aattcaagag aacaaaccag ggtacctgtt ttaatcaaaa
    15961 gcctgctgta agagttggtg ataaggttaa aaagggtgga ataattgcag atggaccttc
    16021 aactgacaat ggacgccttg ctttgggcaa gaatattctt gtggcattta tgccttggat
    16081 gggatataac tttgaagatg cgattctgct atctgaacgt ttagtaaaag atgacgtata
    16141 cacatcgtta catattgaac aattcgaagt tgaagctcga gaaacaaaat tggggcctga
    16201 aaccattacg cgagacatac ctaatcttag cgaaaaggcc tttaaagacc ttgatgccga
    16261 tggagttgta cgtccaggtg catatgttaa acctaatgat attctggttg gaaaagttac
    16321 tcccaaaggt gagcaagata ttactcccga gtacaaactt cttcattcca tattcggaga
    16381 aaaggcgcgg gaggttcgtg atacttctct gcgtgttcca aatggaatgg aaggtgtagt
    16441 tattgatata aaacgctttt cacgagaaaa cggtgatgag ctttcagctg gcgtagaaga
    16501 gcttgttaaa gtctttatcg cgaaaaaacg aaaaatatcg gttggggaca agatggcggg
    16561 tcgccatgga aacaagggtg
//
LOCUS       DAOTGZ010000034        16438 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_3064
            NODE_4256_length_16438_cov_5.017030, whole genome shotgun sequence.
ACCESSION   DAOTGZ010000034 DAOTGZ010000000
VERSION     DAOTGZ010000034.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868088
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 16438)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 16438)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:22:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,785
            CDSs (total)                      :: 2,751
            Genes (coding)                    :: 2,732
            CDSs (with protein)               :: 2,732
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 12 of 19
            Pseudo Genes (internal stop)      :: 5 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16438
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4256_length_16438_cov_5.017030"
                     /isolate="AMR_MDS_3064"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..209)
                     /locus_tag="P5123_03890"
     CDS             complement(<1..209)
                     /locus_tag="P5123_03890"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium transporter"
                     /protein_id="HRX15432.1"
                     /translation="MNTLIVPELRELIASGDYDTLREFLADEHPAVIAEMISALHKDE
                     VWAVLQYAEVDIRSEIYSHLDETLQV"
     gene            complement(528..1430)
                     /locus_tag="P5123_03895"
     CDS             complement(528..1430)
                     /locus_tag="P5123_03895"
                     /inference="COORDINATES: protein motif:HMM:NF013088.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HRX15433.1"
                     /translation="MNHMKNLSQETIGEVFNLGAMLLWGMMPVLVRSSSGIVPPLFFS
                     GSSIFLAGLVCFFVLMVQKKIGEIFCAAAFRDMMMSTLIISVLFYGVLFFAGQKTSAG
                     NIAIIGQSEVVWTFMFFTLLGFEKLTLKKTLGAAVVVCGVLMIVIKSFSGDFVVWDFV
                     LLLVFAITPLGNYFQKRALKRVSSITQLCFRSLVGGSLSLIASLIFERGHFTEASAVP
                     IVVLVLLNGILALGLSKIMFLESLKRLEVSKTIAMGAAAPAVTMTAAYFILNEKPDIL
                     QLLGLALVVLGILVMINKSRSIRL"
     gene            1636..2514
                     /locus_tag="P5123_03900"
     CDS             1636..2514
                     /locus_tag="P5123_03900"
                     /inference="COORDINATES: protein motif:HMM:NF014046.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ARMT1-like domain-containing protein"
                     /protein_id="HRX15434.1"
                     /translation="MKSFIDCIPCFVRQSLDACRMITSDEETIRVLMPKLLAELSSTD
                     LTMPPPAVVSRVHRIIRKELNNNDPYRKLKKQSTQKALELSKSAEDMIAKSNSPFDCA
                     VRFAIAGNILDFGAKTDWNEDRVMNSFKLAMEKQLTDNQVTELYSKIEMAKTVLVLGD
                     NAGEALFDRLLIEKFPGDAKVYYAVKGSPVINDVTEEDALDVDLDKVACIISNGADIP
                     GTMLSECSDEFKQLFKEADVVVSKGQGNFETLNENDREIFFILQIKCASLAHRNGYTE
                     GDWVVTSTTSLKELND"
     gene            2507..2701
                     /locus_tag="P5123_03905"
     CDS             2507..2701
                     /locus_tag="P5123_03905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003322371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005575 - cellular_component [Evidence
                     IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YwbE family protein"
                     /protein_id="HRX15435.1"
                     /translation="MINQKREFIKPGLKVAVVQKQDQRSGALTEGTVESILTNSGFHP
                     HGIKVRLTDGTVGRVKKILE"
     gene            complement(2693..3259)
                     /locus_tag="P5123_03910"
     CDS             complement(2693..3259)
                     /locus_tag="P5123_03910"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF014573.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008753 - NADPH dehydrogenase (quinone)
                     activity [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0050136 - NADH dehydrogenase (quinone)
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase"
                     /protein_id="HRX15436.1"
                     /translation="MIIVYAHPNKEGHCGEILRLVRSLLNDKNISYELLDLYAMHYDP
                     VLKPEEHYTSGHRDVSKENETIQGLFKNESRFIIIYPTWWNGMPAILKGFFDRLMIPR
                     FAYSYEKTIPIKLLSGKAAVFSSFGGPRVFGKFYAGDRSLKQISKDILAFCGIRAKCY
                     AIDNARKLTPSQGKKIERKVNKALSWLL"
     gene            complement(3261..3410)
                     /locus_tag="P5123_03915"
     CDS             complement(3261..3410)
                     /locus_tag="P5123_03915"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRX15437.1"
                     /translation="MSVNDKKTEIKDFEKKRIEKIIKKESDEDSVDALKDLVNRFRDD
                     TLTGR"
     gene            3599..4762
                     /locus_tag="P5123_03920"
     CDS             3599..4762
                     /locus_tag="P5123_03920"
                     /inference="COORDINATES: protein motif:HMM:NF017885.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XRE family transcriptional regulator"
                     /protein_id="HRX15438.1"
                     /translation="MSIGSRIKQRRLNLNYSLDDLSRLLATKGTPISKAALSKYELGK
                     STPKALNLFNICNVLDTTPEYFLKNNTFEINWIAFRKTSKFTVKEENRVKSIAKEQIE
                     AQMFLDGFIDNSKKKITLPKYKISTLEDAETAAEKLRTEWKLNSWPIESITSLLEEKE
                     IFVVDVDSEKGFDGLSGYADGKIPIIITIGKNTADRKRLNLSHELGHLVLDIKNLKEE
                     DTAFRFAGAFLIPKDCLFNALGHKRRHIDIRELIILKEEYGISIQALIRRCRDLDIIN
                     QQEYKRLNIYMRANDLHLVEPANCNNKEIPTKIKSKLFRAIAEGLTTETEVLSRFPGL
                     SREIRGDLMVYSFKDKADEMNRIMEESAKNILDEYIDGGSLSDFEIFDDVMEI"
     gene            4763..5104
                     /locus_tag="P5123_03925"
     CDS             4763..5104
                     /locus_tag="P5123_03925"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008352700.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004521 - endoribonuclease activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system PemK/MazF family
                     toxin"
                     /protein_id="HRX15439.1"
                     /translation="MEPQKGEIWLVNFDPTVGSEVSKKRPAIVMSSNVIGILPLRIIV
                     PVTSWQEKFANNPWLIKIEKNLTNNLNNDSTADAFQVKSISNERFIKKIGSISDSLVN
                     EIKMGIILCIG"
     gene            complement(5227..6867)
                     /locus_tag="P5123_03930"
     CDS             complement(5227..6867)
                     /locus_tag="P5123_03930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012061122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 43 protein"
                     /protein_id="HRX15440.1"
                     /translation="MKTIVNPVLPGFNPDPSIIRVGKDFYIATSTFEWYPGVQIHHSR
                     DLVNWNLVTRPLRRDSQLNMMGEGYSMGVWAPCLSYDDGLFYLCYTDVKEPGTMHNYL
                     VTSDSIEGDWSDPVYLDSRGFDPSMFHDDDGRKWYTVMYLDMVAWNNMMNLNWGMQDT
                     VSAKRILESYDKYQKEFPFFKGILLQEYDHSKKSLVGPVKKIFDKAIGVTEGPHLYKR
                     NGYYYLVTAEGGTSYEHAVTMARSKSIAGPYEIHPQNPVITSKGTDALVQKAGHGDIV
                     ECENGDVYLVHLGSRPVEEKKRCILGRETSIQKMMWGDDDWLRLEAGGNTPLAEVTVP
                     DYGDTKKIIYPDRDDFDSDKLNINFQWLRGDVFGKMASLTERPGFLRLKGREAVYSKY
                     TQALVARRQQSFKYSAQTAMEFDPNEENQMAGLVVYYCNELFYYLYMGYDYSIGAYIS
                     VMSNNLGDLDLFAAGRVAVKADRVYLKAEVNYSELQFYYSTDDVSWIRVGDVLDMSLL
                     SDDVSGGFTGAFVGMACHDPATMKVYADFDYFEYVEKV"
     gene            7121..9394
                     /locus_tag="P5123_03935"
     CDS             7121..9394
                     /locus_tag="P5123_03935"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: protein motif:HMM:NF012492.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HRX15441.1"
                     /translation="MNISQLVDYLKEQPDFMQNVTMWVRNDAKPGKYLKYPDFLSDRL
                     TGYYINKGIKELYTHQRLACDAVQKGEDVVVVTPTASGKTLCYNLPVINHMLDNPEAR
                     ALYLFPTKALAQDQMNEVMNICEALEAPIKTYTFDGDTPGTARKAVREAGNIVVTNPD
                     MLHAGILPHHTMWIKLFENLKYIVIDELHTYRGVFGSHLANLLRRLRRICAFYNCHPQ
                     FILCSATINNPKEHAEKLLERPVTLIDDNGAPQSERHYVVYNPPVVNKQLGIRSSSVN
                     EAAKIGAQILKNKIPLIIFARSRMRVEIISTYLKERCRGVTIKGYRGGYLPNERRAIE
                     KGLREGSIDGVVSTNALELGIDIGMLDAVITTGYPGSISSLHQQFGRAGRRDKPSLAI
                     MVATSSPLDQYFASNPYFLISSNPENAVINPDNLLILMDHIKCAAFELPFKANEIFAE
                     HLGTTREMLKYLEDEGILKQVDNTYHWMNQIYPANEVSLRSAAQENFVIVDTTAKNRV
                     IGEVDYNSAPSLIHDDAIYIHQGKQFYIDKLDWERRTAFCHETDSEYYTDANVKVDIH
                     VIGQFETSDYGKAVLSKGELNVREKAMMFKKIRFKTHENLGWGKIDLPEQEMHTSSCW
                     IDFDEEFLYTFGGKETAGHLLHSLAYLFNNIAPIFTFCDIRDIHTFQMARSNHSGKPS
                     VFIYDTLPGGSGISHRVFEILPLIAQAANESLQKCSCDNGCPGCIGPVSPDSDNIKNK
                     LSQLLELFEINTGDNLH"
     gene            complement(9488..9877)
                     /locus_tag="P5123_03940"
     CDS             complement(9488..9877)
                     /locus_tag="P5123_03940"
                     /inference="COORDINATES: protein motif:HMM:NF022666.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3015 family protein"
                     /protein_id="HRX15442.1"
                     /translation="MKRIICLVLYLIFSTGLFAESAGVILVLSSLSSTGLTAVSALSD
                     MVGKRSARLERMREFVDKNGLYLASDVAGSGNEYLGCVADIAGVKPDKRIIFFRVVRE
                     NYVTVFPDENVDSNYSAGVLIDIAEKL"
     gene            complement(9908..11542)
                     /locus_tag="P5123_03945"
     CDS             complement(9908..11542)
                     /locus_tag="P5123_03945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010940844.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HRX15443.1"
                     /translation="MLQVTDVSLAFNKSPLFKNVNLKFTPGFCYGLIGANGAGKSTFL
                     KILSGEIKPDSGSISHGPDERIAILKQDQFAFEEHTVLDTVFMGYTKLYEIVKERETL
                     YAKGEFSEEDGIRAAELEEIFGEMDGYEAESNAAILLSGLGIMDELHTKKMKELEGGE
                     KVRVLLAQALFGNPDILLLDEPTNNLDLESITWLENFLYNFDNTVIVVSHDRHFMNRV
                     CTHIADIDFQKIQLYAGNYDFWAEVSQLAMRQKKDENKKKEDKIKELQAFVERFSSNA
                     SKAKQATSRKKILDSLTLEDIPATSRRFPWVAFKPERECGKVILEVEGLCKSIDGVEV
                     IKDFDLVVNQKDKIAFVGPNNIAKSVLFDMLCGNMVPDAGTIKWGETITTSYFPKESA
                     SYFDVDYDLINWLRQYSKEKDENFVRGFLGRMLFTGEDALKKVSVLSGGEKVRCMLSK
                     MMLSGANALVLDEPTNHLDLEAITALNNGLIAFTEVILFSSHDHQFIDSIANRIIELT
                     PNGYIDRMMRFDDYIENEDVRKLRDSLYNGHDRIII"
     gene            11780..13480
                     /locus_tag="P5123_03950"
     CDS             11780..13480
                     /locus_tag="P5123_03950"
                     /EC_number="2.7.1.90"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018604412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diphosphate--fructose-6-phosphate
                     1-phosphotransferase"
                     /protein_id="HRX15444.1"
                     /translation="MSTLTDLLIGMEKHKSIFENEIRNLSIPTCSDFGSSENPQPISF
                     TPSGEKLESKDSDKVKQAFQNSFEQAVLKSSGKNPSPLSNKRVAVLFSGGPAAGGHNV
                     VYGLKKILGVNNTLLGVKAGPKGLIQGDLFEITDQNIAPVINTGGFDFLGSDRTKIKS
                     DEQFQSVKKTCIDNNLDAIVVVGGDDSNTNAAILAEFLSKDVKSDGSGVQVIGVPKTI
                     DGDLQIGDILPISFGFDTATKIYAEMVGNILQDTPSSRKYWHFIKVMGRSASHVALEV
                     ALQTKPAIALISEEVAEKSIPLSAIVDQIAKAVVNRSNKGINHGVVLIPEGIIEFIPE
                     MNALIAELNDKVAMFHSELSAKNNEEKRNFMYAKLTPEHAQLMASLPDYIEDMLLADR
                     DSHGNLQVSLIPTERLLIDMVKVRVSELDQSAPFATLNHFFGYEGRCGAPTLFDAAYC
                     YNLGLTAGSLILSGKTGYMASIADLNGGGEPLGIPLPGLLNIERRQGKDEFVIEKALA
                     DTKSPAMEFFTSRREAWASEDLFSSPGPRQLWGPVSRQIPITVALNRKYSSLDFNIGK
                     "
     gene            complement(13596..15626)
                     /locus_tag="P5123_03955"
     CDS             complement(13596..15626)
                     /locus_tag="P5123_03955"
                     /inference="COORDINATES: protein motif:HMM:NF012861.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005247 - voltage-gated chloride channel
                     activity [Evidence IEA];
                     GO_process: GO:0006821 - chloride transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chloride channel protein"
                     /protein_id="HRX15445.1"
                     /translation="MSRISSFLLTIIERFTKTQHSVIIVAAIITGIIAGFAAVGMRLM
                     IHLISDTVFAGNGSFLEAVSRAPWYLVLFIPALGGALVGPITYIFAREAKGHGVPEVM
                     QSLIVKGGIIRARVAVVKALTSSITIGTGGSVGREGPIIQVGASVGSAVGQFFRMSNR
                     RLKTLVGCGAAAGIAAAFNTPVAGALFAAEIILRNFSFYQLSPIVIASVTATVVSHTI
                     MGNYPEFEVPQFAMASYYELFLYFGLALLCAVVAYLFIKILYFSEDFFDEKIKIPIYL
                     KPVLGGLIIGAIGLVFPRILGAGYESIDQALHGDIFWKLALVLLALKLVATPLTLGSG
                     GSGGIFAPSLFLGAMAGAFFGGIVNIIFPGMVSPASAYALVAMGGVVAATTHAPITAI
                     IIVFELTHDYKIILPIMITCMVSVLIVTKFQRESIYTLRLVRRGIKLKEGTDHNVLRS
                     VLVRSVYKSRFISLCVYDKFQDIIDIFIEANQPIIPVLSDGRLKGIISINDIKSHFNK
                     VQNLAGVMVADDIADRDYKTVTLDDNCDTVVEIMRKEKVEGVAVVSADDSSKLLGMIW
                     REDILDVYNTEVRSLEMTGTIADIMTSNNEEEMQVMEKYSIAQIHVPLEFVGKTIREL
                     NVQQNYGIIVLLVFKSDSHKKRRMPEADYCFSKDDMAIIAGDSEFIKRVRDL"
     gene            15920..>16438
                     /locus_tag="P5123_03960"
     CDS             15920..>16438
                     /locus_tag="P5123_03960"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HRX15446.1"
                     /translation="MITFESISLNFDENILFENFNYKFNEGSKTLIHGNSGKGKSTLL
                     RMILGFQTEYRGNIYFNGIPLSPETIALVRSQCAYVSQDVDLPDDTVKKVIDSIFMLK
                     TNRKISPDFDLFNSMAKDFQLPTDILTKPTSELSGGERQRTGLIIAFMLKRPVLLLDE
                     PTSAVESSMKNTI"
BASE COUNT         5372 a         3552 c         2917 g         4597 t
ORIGIN      
        1 acctgcagag tctcgtcaag atgactatat atttcagatc ttatatctac ttctgcatat
       61 tgcagtaccg cccaaacctc atccttatga agagccgata tcatctcggc aataacggca
      121 gggtgctcgt ccgcaagaaa ctctcgaagg gtatcataat cacccgaggc aataagttca
      181 cggagttcag gcacaatgag tgtattcatg gtctcctccc tacggcaaat tatgccgaag
      241 agaaaactcc attaacttat aatatcaggt taacgcgtca tcaatcggca aaaaaattta
      301 ctggtacaat tataacaaat aaagtaattt gagttatctg ctggctgata ctgccaccat
      361 ccgaactgct cattatttcc tctataaaaa atagattttt aactaaagaa acagcattac
      421 tgttattgga tcactcagaa attgattgta atgatgattt tatagtatcg ataaaatcat
      481 cattacactt gtcattttac aagaagttat cgaaatgtca agttacatta aagccgtata
      541 gaccggcttt tattaatcat gaccagaatc cccaatacaa caagagcaag tcccaacaat
      601 tgcaaaatat cgggtttttc attcagaata aagtaggcgg cggtcatagt taccgctggc
      661 gcggctgccc ccatagcaat tgttttggat acttcgagcc tcttgagcga ttcaagaaac
      721 attattttgg acaatccaag cgccaaaata ccattcaaca aaactaacac aacaattggt
      781 acagcagatg cctcagtgaa atgtccccgt tcaaaaatca agctggcaat aagggaaagt
      841 gatcccccca caagacttct aaaacagagc tgagttattg aagagacgcg tttaagtgcc
      901 cttttttgaa aatagttacc caaaggcgtt attgcaaaaa ccagaagaag tacaaagtcc
      961 caaactacaa aatcacctga aaagcttttt attacaatca tcaatactcc acaaactaca
     1021 accgccgcac caagagtttt ctttaatgtt agtttttcaa aacccaaaag tgtaaagaac
     1081 ataaatgtcc agacaacttc ggattgtccg attatggcaa tattgccagc actcgtcttc
     1141 tgccccgcaa aaaagagaac accgtaaaaa agaaccgata ttataagagt actcatcatc
     1201 atgtcacgaa aagctgctgc acagaaaatt tcgccaattt ttttttgaac cattagcaca
     1261 aaaaagcaaa caaggcctgc caaaaatata gagctacccg aaaagaaaag cggaggcact
     1321 atgcctgatg acgagcgaac aagaacaggc atcatccccc aaagcagcat tgcacctagg
     1381 ttaaaaactt cgcctattgt ctcttgtgat aaattcttca tatggttcat taacatctcg
     1441 ttctggttta ttttaaatcc tttaacttat aaagacagtt atcttgttat tctccataac
     1501 atgataattt ttcaaagttt tctgttaata aatttttatc tataattacc agcttcctta
     1561 atataatatc ttgacccata catggattaa gatattagtg ttaactaaca agtttgagcc
     1621 tcaaccggag atattatgaa atcatttatt gactgtatac cctgctttgt gcgccaaagc
     1681 cttgacgctt gccgtatgat aacatctgac gaagagacaa taagagtact tatgccaaaa
     1741 ttattggccg agctttcttc aactgacctg accatgccac ctccggcggt tgtaagccgc
     1801 gttcatcgaa tcattcgtaa agaattaaac aataatgatc cttaccgcaa gctcaaaaag
     1861 cagtcaaccc aaaaggctct tgaactttca aaatcggctg aagatatgat cgcaaaaagc
     1921 aattcaccat ttgattgcgc ggtacgtttc gcaatagccg gaaacattct ggattttggt
     1981 gcaaaaacag actggaatga agatcgcgta atgaactcat tcaaattagc catggaaaag
     2041 caacttacag acaatcaggt cactgaacta tactctaaaa ttgaaatggc gaaaacagta
     2101 ttggttcttg gagacaacgc tggagaagca ctttttgacc ggttgctgat tgaaaaattc
     2161 cccggagacg caaaagtata ctatgccgtt aaagggagcc cggttataaa cgatgtcaca
     2221 gaagaggatg cccttgatgt cgatctggat aaagtcgcct gcataatatc aaacggagcc
     2281 gatattcccg gcacgatgtt aagtgaatgc tctgatgagt ttaaacaatt attcaaagaa
     2341 gctgatgttg ttgtatccaa aggacagggg aattttgaaa cattgaacga aaacgatcgt
     2401 gaaattttct tcatattgca gattaagtgc gccagtcttg cacatcgtaa cggatacacc
     2461 gagggcgatt gggtagtaac atcaacaacg tctctgaagg aactaaatga ttaatcaaaa
     2521 aagagagttt attaaacccg gtctaaaagt ggctgttgtt caaaaacagg atcagcgaag
     2581 cggtgcctta acagaaggca ctgtcgagtc catattaact aactcaggct ttcatccgca
     2641 tggaataaag gtccgtttga cagatggaac agttggtcgg gtaaaaaaga tattagagta
     2701 accaagatag agccttatta acttttcttt cgattttttt accttgtgat ggagtcaatt
     2761 ttcgtgcatt atctatcgca tagcacttag ctctgattcc gcaaaacgcc agaatatctt
     2821 tacttatctg ttttaaagat ctgtcaccgg catagaactt tccaaaaaca cgcggtccac
     2881 caaaagagga aaaaacagcc gccttgcccg aaagaagttt tattggtatt gttttttcat
     2941 acgaataggc aaaacgggga atcatcagac gatcaaaaaa gccctttaaa attgccggca
     3001 taccattcca ccaggtagga tatataataa taaatctgct ctcattttta aacaagccct
     3061 gaatggtttc attttctttt gaaacatctc tgtgtccaga tgtgtaatgt tcttcgggtt
     3121 taagcaccgg atcatagtgc atagcgtaaa gatcaagtag ctcataagaa atatttttgt
     3181 cgtttaacag agatctgacc aaacgtagta tctctccaca atgcccctct ttgtttggat
     3241 gagcgtaaac tataatcata ttatctccct gttaaagtgt catccctaaa gcggttaacc
     3301 agatctttta gagcgtcgac agaatcttca tcagactctt ttttaattat cttttcaatg
     3361 cgtttttttt cgaaatcttt tatttcggtt tttttatcgt ttactgacat ggatactcct
     3421 tttttacgga tttatgatat cttggctata ctcagaatat aaagaattaa atgcaacatc
     3481 aaaatgatac gagtcaatta acgcttattt gttaattatg taaacataag ttgacaaata
     3541 ttgaataata tgttattatg tctacataca aactaacact ttatatggtg aattttatat
     3601 gagcataggt agtcgaatta agcaaagaag actgaatctc aactattctc ttgatgattt
     3661 aagcagatta ttagcaacaa agggaacccc gatttcaaag gctgcattat caaaatatga
     3721 gcttggaaaa agcaccccaa aagctttaaa tctttttaat atatgcaatg ttttagatac
     3781 tacaccagaa tattttttaa aaaacaatac ttttgagatt aattggatag catttagaaa
     3841 aacatctaaa tttactgtta aagaagaaaa tcgagtaaag tccattgcta aagaacaaat
     3901 tgaagctcaa atgttcttgg atggttttat agacaattcc aaaaagaaga taactttacc
     3961 taaatataag atcagtacgc ttgaagatgc tgaaactgct gctgaaaaat taagaactga
     4021 atggaaattg aatagttggc ctatagaaag tataacaagc cttttagaag agaaggaaat
     4081 ttttgtggta gatgtagatt ctgaaaaggg gtttgatggc ctttccggat atgctgatgg
     4141 taaaatacca attattatta ctataggaaa aaacacagcg gatagaaaaa gattaaattt
     4201 atcacatgaa ttaggtcatt tagttcttga tataaaaaat ctaaaagaag aagataccgc
     4261 ttttagattt gccggtgcat ttttaattcc aaaagattgt ttgtttaacg ctcttggaca
     4321 taaaaggcgg catatagata ttagggaact aattatttta aaagaggagt acggcataag
     4381 tattcaggct ctaatcagaa gatgccgcga tctggatatt atcaatcaac aagaatataa
     4441 aagattaaat atctacatga gagcaaatga cctacacctt gtcgaaccag caaattgcaa
     4501 caacaaggaa ataccaacaa aaattaaaag taaattattt agagcaatag cggaaggctt
     4561 aactacagaa acagaagttc tcagcagatt tcctggttta tcaagagaaa taagggggga
     4621 cttaatggtg tatagtttta aagataaagc agacgaaatg aatagaatta tggaagaatc
     4681 tgcgaaaaac atattagatg aatatatcga tggtggttca ttaagtgatt ttgaaatatt
     4741 tgacgatgtt atggaaatat aaatggagcc ccaaaaagga gaaatttggt tggtaaactt
     4801 tgacccaact gttggttctg aagtaagcaa aaaaagaccc gcgattgtga tgagtagcaa
     4861 cgtaatagga attttacctc ttcgaataat tgttcctgta acatcctggc aagaaaaatt
     4921 cgctaataat ccttggttaa taaaaattga gaaaaacttg accaacaatt tgaataatga
     4981 ttcaacagcc gatgcctttc aagtaaaatc aatttcaaat gaaagattca ttaaaaaaat
     5041 tggaagtatc tctgatagct tggtaaatga gatcaaaatg ggcattattc tctgcatagg
     5101 gtaaaattat aagaatttca ctttgtttat ctgaattata tatatcagtt aatacattct
     5161 gataattaca tatttaaaag gtctttccaa aatcaaataa aaaaaaatat atcaaaccaa
     5221 tacagttcaa accttctcca catactcgaa ataatcaaaa tcggcataaa ctttcattgt
     5281 cgcgggatca tggcaagcca ttccgacaaa agcgccggta aacccaccgc taacatcatc
     5341 ggagagaagg ctcatatcca aaacatctcc aaccctaatc caggaaacat cgtcggtcga
     5401 gtaataaaat tgcagctccg aataattaac ctctgctttt agataaactc tatcggcctt
     5461 aaccgcaact cgaccagcag caaaaaggtc aagatcacca agattatttg acataacaga
     5521 aatataagct ccaatcgaat aatcgtaccc catatacaaa taataaaaaa gttcattaca
     5581 ataatagacg accagtccag ccatctggtt ttcttcattt ggatcaaact ccatcgccgt
     5641 ctgtgcactg tacttaaatg actgttggcg tcttgctaca agagcctgcg tatattttga
     5701 ataaacggcc tccctacctt ttaatctaag aaacccaggc cgttctgtta aagatgccat
     5761 ttttccaaag acatcacctc ttagccactg aaaattaata ttcaatttgt ctgaatcaaa
     5821 atcatccctg tccggataaa taattttttt cgtatcacca taatccggca cagtcacttc
     5881 ggccaaaggc gtattgccac cggcctcaag tctcagccaa tcgtcatcac cccacatcat
     5941 tttctgtatg ctcgtctctc gtccaagaat acaacgtttt ttctcttcaa caggtctgct
     6001 tccaagatgc acaagataaa catcgccgtt ttcacactcg actatatcgc catgaccagc
     6061 cttttgcact aacgcgtctg tacccttgga agtaattacg ggattttgcg gatgtatctc
     6121 gtaggggcca gcaatagact tagaacgagc catggtaacc gcgtgttcat aactagttcc
     6181 accttcggct gttacaagat aatagtagcc gtttctttta tatagatgag gcccttcggt
     6241 aacacctatt gccttgtcaa aaattttctt aactggtcca acaaggctct tcttgctatg
     6301 atcatattct tgaagaagta ttcctttaaa aaacggaaac tccttctggt acttgtcgta
     6361 actttcaaga atccgcttag ccgacacagt atcctgcata ccccagttta aattcatcat
     6421 attattccag gcgaccatat caagatacat taccgtatac cattttcggc cgtcatcatc
     6481 atgaaacata gagggatcaa acccacgact atcaagataa acaggatccg accaatcacc
     6541 ttcgatagaa tccgacgtaa caaggtaatt atgcattgtt ccaggctcct taacatcggt
     6601 ataacagaga taaaaaagcc cgtcatcata actcaaacag ggagcccaca cccccataga
     6661 ataaccttca cccatcatat ttaactgaga atcccttcga agtggccttg ttacaagatt
     6721 ccagtttaca agatcacgag aatggtgaat ctgaacccca ggataccact caaatgtaga
     6781 agtcgcgata taaaagtcct ttccgactct aattattgac ggatcaggat taaaaccggg
     6841 caaaacagga tttacaattg ttttcattat tatctccact ataggcatga tcaaagttta
     6901 tttataatat tagcgtgatt actaataatc aaacaaaaaa tgatttgatt aaggaaactt
     6961 ctaaaaatta caatgttcaa gaaggtgata tggtttaagt gcctactcta aagaaatttt
     7021 tcgcaagttc ctttgctgaa aaattaattt tttaaggttg ccttaattaa atactgcttt
     7081 caaactacta cacaaatatt tagcaattat ggtgcaaaca atgaatatct cccaactcgt
     7141 agactatctt aaagaacagc ccgattttat gcaaaacgta accatgtggg tccgtaatga
     7201 tgctaaaccc ggtaaatatc taaaatatcc cgattttctt tcagacaggc ttaccggcta
     7261 ttatattaat aaaggcatta aagagctgta cacccatcag cgccttgctt gtgatgccgt
     7321 tcaaaaggga gaagatgtcg ttgttgttac ccctactgct tctggtaaaa cactctgtta
     7381 taatcttccg gttataaatc atatgctcga taatcctgaa gcccgtgccc tgtatctctt
     7441 ccccacaaag gctctcgctc aagaccagat gaacgaagta atgaatattt gcgaagctct
     7501 ggaagcaccg attaaaacct atacctttga tggagacacc cccggcaccg cacgaaaggc
     7561 tgtacgcgaa gccggaaaca ttgtagtaac aaatccggat atgcttcatg ctggtatttt
     7621 gcctcaccat acaatgtgga tcaagctttt tgaaaatctg aaatacatag taatcgatga
     7681 actgcacacc taccgcggtg tttttggaag ccacctggca aaccttttac gtcgcctcag
     7741 aagaatatgc gctttttaca actgtcatcc gcaatttata ctctgttcgg caacaatcaa
     7801 caatcccaaa gaacatgccg aaaagctgct cgagcgacca gttactctta tcgatgacaa
     7861 cggcgcaccg caaagtgaac gccactatgt agtttataat ccgccggtag ttaataaaca
     7921 gcttggcatc cgctcgtcat cggtaaacga ggccgctaaa atcggcgcgc aaatacttaa
     7981 aaacaagata cctctgataa tctttgcccg gagccgcatg cgtgtcgaaa taatctcgac
     8041 atacctgaaa gaacgctgcc gtggtgtaac aataaaaggg tatcgcggcg gttatcttcc
     8101 taacgaaagg cgtgcaatcg aaaaaggcct tcgcgaaggt tccattgacg gtgttgtttc
     8161 gactaacgca cttgagcttg gcatcgatat tggcatgctc gatgcggtta ttacaaccgg
     8221 ttatccgggt tccatttcat cgcttcacca gcaatttgga agagccggtc gtcgcgacaa
     8281 accttctctt gcaataatgg ttgcaacatc ctctccgctg gatcagtact ttgcatctaa
     8341 tccctatttt ttaatcagct caaatccgga aaacgcggtt attaatcccg ataatctttt
     8401 aatattaatg gaccatataa aatgcgccgc atttgaactt cctttcaaag ctaatgaaat
     8461 atttgccgag catcttggaa caacccgcga aatgttaaaa tatctcgaag acgaaggaat
     8521 acttaaacag gtcgataaca cctaccactg gatgaatcag atatacccgg caaacgaggt
     8581 gagcctgcgt agcgctgctc aagaaaattt tgttattgtc gatactaccg caaaaaaccg
     8641 ggtaatcgga gaggtcgatt acaactctgc tccgtctctg atacatgatg acgcaattta
     8701 catacatcaa ggcaaacaat tttatatcga caaactggac tgggagcggc gaacagcctt
     8761 ctgtcacgaa acggactccg aatactatac cgatgcaaat gttaaagttg atatacacgt
     8821 aatcggtcaa ttcgaaacct ctgactatgg caaggcggtt ctttcaaaag gagagctgaa
     8881 cgtcagggaa aaagccatga tgtttaaaaa aataagattc aaaactcacg aaaatcttgg
     8941 ctggggcaaa attgatttac cagagcaaga gatgcacact tcaagttgct ggatagattt
     9001 tgatgaggag tttctttaca ccttcggcgg caaagaaaca gcagggcatc ttcttcactc
     9061 tcttgcctat ctttttaaca atatcgctcc aatttttacg ttttgcgata tccgggatat
     9121 acatactttt cagatggctc gttctaacca cagcggcaaa ccgtcagtat ttatttacga
     9181 cacactaccg gggggatcag gtatttcaca tcgagttttc gagatacttc cactgatagc
     9241 acaggctgca aacgagtcgt tgcaaaaatg ctcctgcgat aacggctgtc ccggatgcat
     9301 cggaccggtt tcacccgatt cagacaatat aaaaaacaaa ctgtctcagc ttctagagct
     9361 atttgaaata aatacaggcg acaatttgca ttaataattt ttactaatca gcatgcgagc
     9421 cataagagct gcaatttatt attttttaac aggataattg cgaaatattt ctttgtgcaa
     9481 ataaaactta tagcttctct gctatatcga taagaacacc ggccgaatag ttgctatcaa
     9541 cattctcgtc gggaaaaaca gtaacataat tttcacgaac aactctgaaa aatattattc
     9601 ttttatccgg tttcacaccg gcaatatcgg caacacaacc aagatactca tttccgcttc
     9661 cggctacgtc tgacgcgaga tagagtccgt ttttatctac aaactcacgc atcctctcta
     9721 aacgcgcgct tcttttgcct accatatcag aaagcgccga aacagcagtc aacccagtcg
     9781 aagacagtga agacaaaacc agtatcaccc cagcagattc ggcaaatagt cctgttgaaa
     9841 atataagata taaaacaagg caaattatcc ttttcattta tgcccccgta aaaaccgaaa
     9901 atatgaatta tatgattatt ctatcatgac cattataaag agaatcacgc agcttacgca
     9961 catcttcgtt ttcaatatag tcatcgaaac gcatcatcct gtctatatat ccattaggag
    10021 ttaactcaat tattctgttt gctatagaat caataaattg atggtcatga gatgaaaaaa
    10081 gaataacctc tgtaaaagcg attaatccat tgtttaacgc tgttatggcc tcaaggtcca
    10141 agtggttggt aggctcatca agcaccaaag catttgcccc acttaacatc attttagaga
    10201 gcatgcagcg caccttctct ccccccgaca aaaccgatac tttttttaaa gcgtcctcac
    10261 cagtaaaaag catgcgcccc agaaaaccac gaacaaaatt ctcatctttt tctttcgaat
    10321 attgacgtaa ccagtttata agatcgtagt caacatcaaa atacgacgcg ctctccttag
    10381 gaaaatagga tgtcgttatt gtttcgcccc attttattgt accggcatca ggaaccatat
    10441 taccgcaaag catgtcaaaa agaactgatt ttgctatatt gttcggcccg acaaaggcaa
    10501 tcttgtcttt ctgattaact acaagatcaa aatcctttat cacctctaca ccgtcaatac
    10561 tcttgcaaag cccttctacc tcaagaataa cctttccaca ctcacgctcc ggtttaaatg
    10621 ccacccaggg aaaccgccgc gatgtggctg gaatatcttc cagagttaaa ctatcgagaa
    10681 ttttttttcg tgatgttgcc tgtttggcct ttgaagcatt agagctgaac ctttctacaa
    10741 aagcctgaag ttctttgatc ttatcttctt tctttttatt ctcgtctttc ttctgacgca
    10801 ttgcaagctg gctaacctcg gcccaaaaat catagttacc tgcatacagc tgtatcttct
    10861 ggaaatctat atcagcaata tgagtacaaa cacggttcat aaagtgacga tcgtgagaaa
    10921 cgacaataac cgtattgtca aagttataca aaaagttctc aagccatgta atagattcaa
    10981 gatccaggtt gtttgtgggc tcatcaagaa gaagaatatc cgggtttcca aaaagcgctt
    11041 gagccaaaag cacacgaacc ttttcaccac cttcaagctc tttcatcttt ttggtgtgaa
    11101 gctcatccat aatgccaagc cccgacagca gaatcgcggc attagattcg gcttcataac
    11161 cgtccatctc tccaaatatc tcttccagtt cggcagctct tattccatct tcctcactga
    11221 attcaccctt ggcataaaga gtctcacgtt ctttaacaat ttcgtacagc ttggtatacc
    11281 ccataaaaac agtatccaat actgtatgct cttcaaacgc aaactgatcc tgctttaaaa
    11341 tcgctattcg ctcatccggc ccatgcgaaa tagaaccgct atccggtttg atttcaccgg
    11401 ataatatttt taaaaaagtt gacttgcccg cgccattggc tccaattaga ccatagcaaa
    11461 atcctggagt aaactttaaa tttacatttt taaatagagg cgatttgtta aacgccaaag
    11521 aaacatctgt tacctgaagc atatattacc tgttttttat taaaattcga gcccttaagc
    11581 tttataacca ctacaccaat gaagagctta acggtcaagc ccttcattgc ctttttattg
    11641 ccttccggct ccaaatttat cttgacacta accttagccg cttttcaatt caaaaaaacg
    11701 ggataccgta catttttaac actcaatctc ttcaatacgg tcttgtcccg gtaaaaaaca
    11761 gttaacgatg gagacaataa tgagcacatt aacagatctt cttatcggca tggaaaagca
    11821 taaatcgatc ttcgaaaacg aaattcgcaa tctttccatt ccgacatgtt cggattttgg
    11881 atcatccgaa aatcctcaac caataagctt cacaccgtct ggtgaaaaac tcgaatcaaa
    11941 agatagtgat aaagtaaaac aggctttcca aaattcattc gagcaggctg ttctaaaatc
    12001 ttcgggaaaa aacccttctc cgctctcaaa caagcgtgta gcggttctct tttccggtgg
    12061 acctgcagcc ggcggccaca acgttgttta tggactaaaa aaaatcttgg gtgtgaacaa
    12121 tactcttctc ggagtaaaag ctggccccaa gggtcttatt cagggtgacc ttttcgaaat
    12181 aaccgatcag aatattgcgc ctgtaataaa tactggcggt tttgactttc ttggtagcga
    12241 ccgcacaaag atcaaaagcg atgagcagtt tcaaagtgta aaaaaaacct gtatagacaa
    12301 caaccttgac gccattgttg ttgttggcgg tgatgattcg aacacaaatg cagctattct
    12361 tgcagagttt ttgtcaaagg acgtaaaaag tgacggctcg ggagtacagg taattggtgt
    12421 tcccaaaaca atcgacggag accttcaaat cggcgacata cttccaattt ctttcggttt
    12481 tgatactgcc acaaagatct atgccgaaat ggtaggaaac attcttcagg acactccatc
    12541 ttctcgcaaa tattggcact ttataaaggt tatgggaaga tccgcttcgc atgttgccct
    12601 tgaggttgcc ttgcaaacta aacccgcgat cgccctgatt tcagaagagg ttgccgaaaa
    12661 atcgatacca ctttctgcca ttgttgatca gatagcaaaa gcggttgtaa accgctcaaa
    12721 taaaggcatt aatcacggag ttgttcttat tcctgagggc atcatcgagt tcattccgga
    12781 aatgaacgca cttatcgccg agcttaacga taaagttgct atgtttcatt cagagttatc
    12841 tgcaaaaaat aatgaagaga agcgtaactt catgtacgcc aagcttactc cagaacacgc
    12901 tcagcttatg gcatctcttc cggattatat cgaagacatg ctgcttgcgg atcgcgacag
    12961 tcatggaaac cttcaggttt cacttatacc aacagagcgt cttttgattg atatggtcaa
    13021 ggtgcgtgta agcgagcttg atcagtcggc tccattcgct actctaaacc actttttcgg
    13081 ttatgagggt cgttgcggtg ctccaactct ttttgacgca gcttactgtt ataaccttgg
    13141 actaaccgca ggctcgctta tactgtctgg taaaaccgga tacatggcaa gtattgctga
    13201 tctaaacggt ggtggagagc cacttggtat tcctcttcct ggccttttaa acattgaacg
    13261 ccgtcaaggt aaagatgagt ttgttatcga gaaagctctt gccgacacca aatcaccagc
    13321 aatggaattt tttacttcaa gacgcgaagc atgggcaagc gaagacctgt tttcttctcc
    13381 aggaccgcgc cagctttggg gaccggtatc acgtcaaata ccaataactg tagccctaaa
    13441 cagaaaatac agcagtctgg attttaacat aggcaaataa gcctctatat ttccactgac
    13501 tgattaataa taaaaggtga tccagttggg tcacctttta tttattcaat agcatttggg
    13561 atcctctaat aattagctca tcttatcaaa tttttttaaa ggtcacgaac ccgttttata
    13621 aactcactgt ctcccgcaat tatcgccata tcatctttac taaagcagta atcagcctcg
    13681 ggcattctgc gctttttatg gctatcagac ttaaaaacca gcaagactat tattccatag
    13741 ttttgctgca cattcaattc acgtatggtt ttaccgacaa actctaatgg aacatgtatc
    13801 tgagcaatgg aatacttttc catgacctgc atctcttctt cattattcga agtcataatg
    13861 tccgcaatcg ttcccgtcat ttccagactg cgaacctcgg tattgtaaac atccagaata
    13921 tcttcacgcc atatcattcc taaaagcttc gaggagtcat cagcagatac aacagcaacc
    13981 ccctcaacct tctctttccg cataatttcg acaactgtat cacagttgtc atcaagagtt
    14041 accgttttgt aatctctatc tgcaatatca tcggcaacca ttaccccggc aagattctga
    14101 accttattga aatgactttt tatatcatta atacttatta taccttttaa acgaccatca
    14161 cttaacacag gtataatagg ctgatttgct tcgataaaaa tatcaattat gtcttgaaat
    14221 ttgtcgtaca cacacaaact tataaaccgg cttttgtaaa ccgaacgaac cagaaccgaa
    14281 cggagcacat tgtgatcagt gccctcttta agctttatgc ctcgtcgtac aagacgaaga
    14341 gtgtagatag actctctttg aaattttgtt actattaata cactcaccat acaggttatc
    14401 attattggca agataatctt ataatcgtgt gtaagctcga acactataat gatagccgtt
    14461 ataggagcat gtgtcgttgc ggcaacaact ccacccatgg caacaagagc ataggcactt
    14521 gcaggcgaga ccattccggg aaaaattata ttaacaatac cgccaaaaaa agcccctgcc
    14581 atagcaccta gaaaaagtga tggcgcaaaa ataccaccgg accctccaga gcccaaagtc
    14641 aaaggagttg caacaagttt taacgccaac aaaacaagtg ccagtttcca aaaaatatca
    14701 ccatgaagag cctggtcaat cgattcgtaa ccggctccca gtatacgagg aaaaacaagt
    14761 ccaattgcac cgataatcag accaccaagt actggtttta aatagatagg aatctttatt
    14821 ttttcatcga aaaaatcttc tgaaaagtag agtatcttaa taaaaagata tgcaacaaca
    14881 gcacaaagaa gagccaagcc aaaataaaga aatagctcat aatagcttgc catagcaaac
    14941 tgtggcactt caaattccgg gtaattaccc atgattgtat gtgaaacaac tgttgctgtt
    15001 accgaagcta ttacgatagg agagagttga taaaacgaaa aattccgcaa tattatctcg
    15061 gcagcgaaca gggctcccgc gacaggtgta ttaaatgccg ctgcaatacc cgcagctgca
    15121 ccacacccga caagagtttt taatctgcgg tttgacattc taaaaaactg tcctaccgcc
    15181 gaaccgacac tcgcgccgac ctgaataatc gggccttcac gccccacaga acctccagtt
    15241 ccgattgtta tagatgaagt caaggcctta actacagcca cccgcgcccg tataatgcct
    15301 cctttaacta tcaaagactg cataacctcg ggaactccat gcccctttgc ttctcgtgca
    15361 aagatatatg ttatcggccc aacaagcgcc cctcccagag ctggaataaa aagaacaaga
    15421 taccagggag cacgactgac agcttcaaga aaacttccat tcccagcaaa aaccgtatcc
    15481 gaaatcagat gtatcataag ccgcatgcca acggccgcga aaccggcgat aatacccgta
    15541 ataattgcgg caactattat taccgaatgc tgtgtttttg taaagcgttc aataatagtt
    15601 aatagaaacg atgaaatacg gctcatcaaa acctccgagg aaaaatcaaa agatgcaata
    15661 aattttattt aatagagact agtgtcaaga gtaaattact agcctataat aatatgaatt
    15721 tctaaaccaa atttttcaaa attatttaaa ctatttctct tgtttgaata aagacattca
    15781 aaacttttaa ttaaagaata ttgcttttaa agaaaaaacg gttgcataag attaaaagaa
    15841 aaaaaatata agacacttaa atacatttga aaagtattga aactaaaaga taccctcttt
    15901 caaataaaca ggaacaatca tgataacttt tgaatccata tctctaaact ttgacgagaa
    15961 tatacttttc gagaatttca actataagtt taatgaaggc tcaaaaacac ttatacacgg
    16021 taattccgga aaaggtaaaa gcacacttct tcgaatgatt ctgggttttc aaaccgaata
    16081 cagagggaat atatacttta acgggatccc cctgtcacca gaaaccatag ccttggtacg
    16141 gtcacaatgc gcctatgtaa gccaggatgt tgaccttccc gacgacacgg ttaaaaaggt
    16201 aattgattct atttttatgc ttaaaaccaa ccgtaaaatt tcacccgact ttgatctttt
    16261 taactctatg gcgaaagatt tccagttgcc aacagatatt ctcacaaaac ccacatcaga
    16321 gctatcgggt ggtgaaaggc aaagaaccgg actgattatt gcatttatgc ttaaaagacc
    16381 ggttctgctt cttgacgagc caacttcagc tgtagaatca agtatgaaaa acacaata
//
LOCUS       DAOTGZ010000035        16216 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_3064
            NODE_4328_length_16216_cov_4.892890, whole genome shotgun sequence.
ACCESSION   DAOTGZ010000035 DAOTGZ010000000
VERSION     DAOTGZ010000035.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868088
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 16216)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 16216)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:22:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,785
            CDSs (total)                      :: 2,751
            Genes (coding)                    :: 2,732
            CDSs (with protein)               :: 2,732
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 12 of 19
            Pseudo Genes (internal stop)      :: 5 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16216
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4328_length_16216_cov_4.892890"
                     /isolate="AMR_MDS_3064"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1156
                     /gene="mgtE"
                     /locus_tag="P5123_03965"
     CDS             <1..1156
                     /gene="mgtE"
                     /locus_tag="P5123_03965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012173841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015095 - magnesium ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015693 - magnesium ion transport [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="magnesium transporter"
                     /protein_id="HRX15447.1"
                     /translation="TLRRDEIVKLLTHMSPDDRADLFKHMPEELGESILPAMAHVERE
                     DIRRLSSYEEGTAGAVMTSDYATLPPGLTAQQAIDHLRQVAPNKETIYYSYVIDEYRH
                     ILGFVSLKDLIVSHKDSLIDDIMNAEVITARVGDDQEDAARNIQKLDLIALPVLNEQD
                     ALVGIITHDDAIDIITQEHTEDMEKFMAISGSHEDAVYMKMTILNHFRNRAPWVVVLA
                     IFGLFSGFIVQNFEGLLMQFAILSVFMPMLAGTGGNTGSQSATLVVRALALKEICARD
                     TIRILSKELGIAFLLATLLGLLAYGRVIFFGAGSYIPPQYGLSQIAMAISIALGLQVV
                     SSTLIGALLPLGASKFKLDPALVAIPALTTIVDITGLVIYFTTVKLLLNI"
     gene            1256..3754
                     /locus_tag="P5123_03970"
     CDS             1256..3754
                     /locus_tag="P5123_03970"
                     /inference="COORDINATES: protein motif:HMM:NF013188.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation:proton antiporter"
                     /protein_id="HRX15448.1"
                     /translation="MNIFPVHDPVLIFSIVMLSVLIAPLVSKRLKLPGIVGLIIFGVI
                     LGPHVVNLLERDKTIELLGTIGLLYIMFQAGLEINLEEIKRNKHHSIIFGILTFLIPL
                     FLGTVSAYYILDMNLPASILLASMFSSHTLLTFPIVSRLGLSKKKAVAATVGGTIITD
                     TLAFVVLAVIIAMNSGGLTLLFWPKLIGYSIVYTASVVILLPRLTAWFFRNYFNDSGV
                     EDYVFVLTAVFIAAFFSHLIGLEPIIGAFLAGLTLNPLIPEKSVLMNRIQFVGNALFI
                     PFFLISVGMLIDPVVLYTNTTAVKVSAVMIVIALLSKDLAARLFAKISSFSKNNRGLI
                     FGMSVSQAAATLAAVLVGYRVGIFSADVLTGTIMMIIVTCFVGSVVTQRYAKKIILQE
                     TQSPSESETGRVDRILIPIARESNVTGLMDFAFLLHSKTGQEPLYPLHVTVDSSDVQQ
                     GIIEGETILTKAGIRASAVQKTTLPLNKIDTNISSAILRAIDEQRISKIVMGWNESGK
                     LTGSVLNTIAEQIAKNCSRMIYIYKHIKPVNTMNRIVLIVPPYIYKQYGFNETISSLI
                     GLSGAISADWLIIAEEESNEDIAVYFNKLQKKVNTYNILSWKNLSGHLKNVIGADDFI
                     IQLVPRRGSISWRLDFEKMPTVLCRSFPNNNLLALYPSTVSDDSYETIVSDDYDVSKP
                     SNLMGELLPVSNILFNLDYDDPSRAISQIADIIGTANRNQIFNTINSVLNEYPLELTE
                     DVVLVHSRTDLLDDYLLFTAVSNQGFVYNTLSEGGRYRIMIVLLSPLSKSTQSHLNVL
                     SQISRFVMVQKMIDALLISENRDQFLKHVSVEDS"
     gene            4040..4762
                     /locus_tag="P5123_03975"
     CDS             4040..4762
                     /locus_tag="P5123_03975"
                     /inference="COORDINATES: protein motif:HMM:NF015437.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LuxR family transcriptional regulator"
                     /protein_id="HRX15449.1"
                     /translation="MNLNDFIHCTNRAQSFEEVVFLFEKFISQYGFTNYVMGDMSLDS
                     PVQRAESFGTLTNYSPEWLEHYKNNNYVAVDPVIKKALISHVPFTWEEAKCEDKTQDS
                     LNMMNEAIEFNLVNGIGLSVHRPFGRIIGFGLATSEKNPHMDKDTMAILHSVCVHFCN
                     VYENLIGINISEYPVCLTVREKEVLYWISRGSTKKNIAEHFKVSESCVKRHCENILQK
                     LSVKNLAHAVLKGVRSGLISPY"
     gene            5033..5668
                     /locus_tag="P5123_03980"
     CDS             5033..5668
                     /locus_tag="P5123_03980"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRX15450.1"
                     /translation="MKISEEVKKVLNAIDKALLLKPFFRVLQNGAFFKVGYSWVILRV
                     GGCFFVLFFLIKWIMVWPDVFKLSEMPNIMLLAILLMLVSLIVVYAIFNAIMVKADEI
                     CAISTEKDYIVIIIVTKIIKLAGEISAFVIWFLGLIIFLLGSSEFGADLLSGIITFDF
                     MEEFSAGAGSAFAYLIKFGFYGFFALAASYFIADLIGVMVDTANNTKKMDR"
     gene            6055..6588
                     /locus_tag="P5123_03985"
     CDS             6055..6588
                     /locus_tag="P5123_03985"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRX15451.1"
                     /translation="MKKNQFEYPAKIVFKRGIKKTIRNISICILLCFVSYAGINDTFG
                     PTPLYIRVISYAIPLIVVYLVIIIIWSELLKKYYVIVDEQRVIYNSLFRKKTFYWVDV
                     ISWSYTKLASENYITFQTRQSVNEYNNSTLLYKIFVKNNNTAFGINLDMCQNPDRATV
                     LKIIEEILLYHGWSRTN"
     gene            6895..7554
                     /locus_tag="P5123_03990"
     CDS             6895..7554
                     /locus_tag="P5123_03990"
                     /EC_number="1.11.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012280978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008379 - thioredoxin peroxidase activity
                     [Evidence IEA];
                     GO_function: GO:0051920 - peroxiredoxin activity [Evidence
                     IEA];
                     GO_process: GO:0098869 - cellular oxidant detoxification
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peroxiredoxin"
                     /protein_id="HRX15452.1"
                     /translation="MDNQSSMPNMPLLGDDFPELSVQTTHGPMNIPGDLKGSWFVLFS
                     HPADFTPVCTTEFVAFQARYDEFEKLGCKLIGMSVDQIFSHIKWVQWIKENLDIEIKF
                     PVVAANDAVALKLGMLHPGKGTNTVRAVFICDPQGKVRLIMYYPQEIGRNMDEVVRAV
                     KALQISDSQGAVPAGWPDNELIKDRIIVSPPKDEKTAAERMKDSSLECYDWWFCHKPL
                     K"
     gene            7771..13503
                     /locus_tag="P5123_03995"
     CDS             7771..13503
                     /locus_tag="P5123_03995"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HRX15453.1"
                     /translation="MPKGEKSAVDCLSNSKLLSKKAPFAYVCHQIMRDKDGKFSNYKI
                     IEVNSVFETISGFKTKNIINKNVSTLISETSEQSFDWKTFYKDILDHNKNKINEQTLN
                     LLDRWYKTQSCMLDNDTLLVMFVDITKEKVQLDELENFFSVNLDLLCIADTDGKFLKV
                     NREWEKVLGYSVDDLEGTRFLAFVHPDDIEKTMSAINMLASDEKVLNFTNRYKDIKGD
                     YHYIEWRSHPHGDLIYAAARDITQQKEAELDLRKSRDRFQSLVQNIPGNVYRCLYDED
                     WTMLYIGRDFDNISGYPSIDFINNSKRSFESIIYEDDRSMVRHEIDRAIEQKKFWNIE
                     YRVKRKDCGLSWVQERGRAVYDSNGCIEYLDGFMLDITEQKLFKEQMVVNENRFRQLF
                     NNMSSGVAIYEVVDSGNDFVFKEFNKAAETIEGVYKADVVGKKVCEVFPSIKNFGLLD
                     VFKRVWKTGVAENHPVSLYHDGRIDGWRENFVTRLSENEIVSIYEDVTEKKRAEKALI
                     QSKEQYELAISGTNDGIYDWDMHSGYLFLSKRWKEIIGYSEDELEYNIETFYSLICDE
                     DKRRVEKHLSDYLEGEIEKYSIEFRMKHKDGSIRWILARGEVVRDEKGVPYRMAGSHS
                     DITEQKNAQKELEESRYRLELVMDAGEHGFWDWNLETKDFFFSPTYFTMLGYDNNELP
                     MSLDLFASLIHPDEREYVLRSIDESIKKGETYDLEFRLLCKSGYYKWVSAKGKGYVDA
                     QTGKVVRAIGIHIDIDERKKSEERIRKSEEQFRLLFEQSPLGIYIAREDGTILDANKK
                     LLELLGSPSVEATKSINVLKFEPLVKNGYADSFIKCIKTKSMVKLELDYKSKWGLDNY
                     YSSYLIPLVDNEGRASIVYTLMENITDRKNIEEKLKASERNFRTFFETMDDMIFIGDK
                     KGNIFYTNKVVENKLGYSREELASMHMLDVHPNEKRKEAENIFERMFAGSLAVCPLPL
                     QKKDGALIAVETRIWFGQWDGKECIFGISKDISAEQAALQKFNKLFNNNPALMAVTSV
                     PDGRFVDVNDSFVKVLGYSKNEVLGVLSRDINIFGNNDTLDKIGRQFKEKGSVKNLEV
                     RVCKKNGELLDGLFSGELIETQGQSYYLTVLIDITARREAIEALRKSEENQSILLDNI
                     QTQVWYLTDPVTYGAVNSAHAEFSGKSKEDLAFKNLYDIFPKENADICSLSNEEVFSQ
                     GHAVFTEEWMPHHSGDNRLLSIAKIPRLREDKTVEYVVCSAEDITERKIAEDNLVIAL
                     QQSDILRERAEEATKAKSQFLANMSHEIRTPLNGVIGFTELLLKTPLNDVQQQYAYNA
                     NTAGQTLLAIINDILDFSKIEAGRLDLDLVESDILLLLDECADIIKYHASQKGLELLL
                     DIEPGMPRYAMLDPVRLKQILINLLGNAVKFTGSGEVELTVKFNRIDDKNGIYNFTVR
                     DTGIGISREQQNKLFKAFTQADSTTTRKYGGTGLGLVISSLLVEKMGGKLTVESKEGK
                     GSIFQFEISTDYSLNKAANDKMNLKKIDVKNVLIVDDNGRNRMILRHNLEYLGINCEE
                     AWDGKSAIEMVRGGRSFDLYIVDYLMPGMNGLETIIRLRKETDALKKTAVILYSSADE
                     IKNSADLKNNGVNYSLVKPVKADDLIKILQGIGQADSVTEEKLNNSFVDVEEPVSKDK
                     INILVAEDVIMNVELMKAMLLEYVPNANIEVALDGHEAVNAVKNKLFDLVFMDVQMPR
                     LDGVEATKQIRENVDNKKIPIVALTAGATKTDRDKCLESGMNYFLGKPVNSTQLKEIL
                     RAILVSGHDDKVFLDTGSFDKKNLFDRLHGNMENYNKILEIAYKQFEQDIVELEQVYD
                     LADYDNLLKKIHRLKGASLNMGFVNLAELVKQFETAAKEDPTGDHSIKQIIEEWMLLK
                     KMLGKELGYE"
     gene            13496..13885
                     /locus_tag="P5123_04000"
     CDS             13496..13885
                     /locus_tag="P5123_04000"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HRX15454.1"
                     /translation="MSENAKKKILLVEDVMTNRFLLESFLTDYDCVSVGNGDEMYVEL
                     EKDRPDLILMDIGLPGENGLELTKQLARNPEFGDIPVIFLTSHSDKREILEAAKAGGY
                     DYILKPADDSILSARIEMALARHKKNK"
     gene            13909..14811
                     /locus_tag="P5123_04005"
     CDS             13909..14811
                     /locus_tag="P5123_04005"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009975 - cyclase activity [Evidence IEA];
                     GO_process: GO:0009966 - regulation of signal transduction
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HRX15455.1"
                     /translation="MDYQEKPSVMVVDDEASNVFLLKNILTDYTVTCAFSAQEMFENI
                     DAAAPKVIILDVMLPDQNGFDIAKKLSSDKRYSSIHIIFLTAKTESTDVVSGFKHGGY
                     DYIKKPFDEIELLARVEAAMKRAFEREKLESEVFIDPLTELYNRRYLTDYIRSESSRA
                     LRSKENFAVAMLDIDHFKSINDNLGHPCGDHILREFARIIADELREYDVAIRYGGEEF
                     LVVFPGESKINAVSVLERIQDKVLSGSYIWEDTPVSFSFTAGIADFMEIDHTSMPFED
                     LINKADHRLYHGKHDGRNCIVIND"
     gene            complement(14989..>16216)
                     /locus_tag="P5123_04010"
     CDS             complement(14989..>16216)
                     /locus_tag="P5123_04010"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRX15456.1"
                     /translation="AAIRLTNGTQSGTVSITDSFIRGGDGNTNAWAICFNDESLSLNE
                     FSFIIERNDIAAGKGNTPNKENAGIEISSINSTLLNDITDTTIDISDNERICGPAQNT
                     NSYDHGIYIKPLSNSTFSNFKVTIQNNGIISAGSVITTNAGYKYGIRFRSTNMPTNTL
                     GSGIIIHNNEMIRSHNIDSTGQPLNITTVFTYSTDTDISVKIYNNHIIAETFHSDVSR
                     ATAISCDNFKNFECINNIITSELSSGNASRGLYIAGGNNSVANVYNNLIYVLGSNGGD
                     AIFDYYQNPDSVNIKNNIIMLNNSLNYCIFSYSSNNRFIQNNYLCPYNNQFYQLDSGI
                     SYNNISSFETDIPSCVNNFTGSSPTFDSNWRLTSASENDPLKSDGIGISGVVSDIDGN
                     PRPGSDGTFAVGPYEY"
BASE COUNT         5091 a         2506 c         3420 g         5199 t
ORIGIN      
        1 gaccctacga cgcgatgaga ttgtcaaact tctaacacac atgtctccgg acgacagggc
       61 tgaccttttc aaacatatgc ccgaagagct tggcgaatca atacttcccg caatggcgca
      121 tgtagagcgc gaggatatac ggcgtctctc ttcttacgaa gagggaaccg cgggcgctgt
      181 tatgacatcg gactatgcta ccttaccgcc agggcttaca gcccagcagg caatagatca
      241 tcttcgtcag gtcgcgccaa acaaagagac aatttattat tcttacgtga tagatgaata
      301 tcgtcatatt cttggttttg tttctttaaa agatctgatt gtatcacata aagactcact
      361 gatagacgat atcatgaatg cagaggttat tactgcacgc gttggcgatg atcaggaaga
      421 tgccgctcgt aatatacaaa aacttgacct aatcgctctt ccggtattaa atgaacaaga
      481 cgcacttgtt ggaataatca cgcatgacga cgcaatagat attatcacgc aggagcatac
      541 cgaagacatg gagaaattca tggctatcag cggtagccat gaagatgctg tttatatgaa
      601 aatgacaata ctaaaccatt ttcgcaaccg agcgccttgg gtagttgtgc ttgcgatttt
      661 tggactcttt tccggtttta ttgtgcaaaa ctttgagggt ttgcttatgc agtttgcaat
      721 tctttctgtg ttcatgccca tgcttgcggg aacaggtggt aataccggta gtcagtcggc
      781 tacacttgtt gttcgtgcgc ttgcattaaa ggagatctgt gcccgagata caatacgtat
      841 tttgtctaaa gagctcggga tagcttttct attggcgact cttctgggtt tgcttgctta
      901 tgggcgagtg atattttttg gggctggttc gtatattcct ccgcaatacg gactgtcaca
      961 aattgccatg gcaatatcaa ttgcacttgg tcttcaagtt gtctcttcta ccctgatcgg
     1021 agcattgctt ccgcttggcg catctaagtt taaacttgat ccggctcttg tggctattcc
     1081 ggccttgaca acaatagtgg atattaccgg tcttgttatc tattttacaa ccgttaagtt
     1141 gctgttgaat atatgattgc tttctgtcaa taatcgaagc caattatatt aggtgagctt
     1201 cgataaaggt attattttta gaaattcctt attaagccca tagtcagagg tttttatgaa
     1261 tattttccct gtgcacgatc ctgtacttat tttttcaatt gtaatgctct cggttcttat
     1321 cgcgcctctg gtctctaaac gattaaaatt acccggtata gttgggctta tcatttttgg
     1381 tgtgattcta gggcctcatg ttgttaatct gttggaacgc gacaagacaa tagaacttct
     1441 tggaacaatt ggtcttttgt acataatgtt tcaggctggg ttagagatca acctggaaga
     1501 gatcaagcga aacaagcacc atagtataat atttggtata cttaccttct tgatcccctt
     1561 gtttcttgga acagtctctg catactatat tcttgatatg aatcttccgg catcaattct
     1621 tttagccagt atgttctctt cgcatacact tttgaccttt cccatagtta gccggcttgg
     1681 tctttcaaaa aagaaggcag ttgccgcaac agttggtggt acaataataa cagacacttt
     1741 agcttttgtt gtgcttgcgg ttattatcgc aatgaatagt ggcggactca cactgctttt
     1801 ttggccaaaa ttgattggat actccattgt ttataccgca tctgttgtta tacttctgcc
     1861 tcgattaaca gcctggtttt ttcgtaacta ttttaacgat tccggagtgg aggattatgt
     1921 ctttgtattg actgctgttt ttatcgcggc gtttttttcg catcttatag gtcttgagcc
     1981 gatcattgga gccttccttg ccggactaac gcttaaccca cttattcctg aaaaaagtgt
     2041 gcttatgaat cgtatacagt ttgtgggaaa tgctctcttt attccgttct ttcttatctc
     2101 tgtaggaatg cttatagatc cggtagtgct ctatacaaat acaactgccg taaaagtttc
     2161 ggcagtaatg atcgttattg ctctattatc taaagattta gccgcgcgac tgtttgctaa
     2221 aatttcgagc ttttctaaaa ataatcgcgg gttgattttt ggtatgagtg taagtcaagc
     2281 tgctgctact cttgcggctg tccttgtggg atatcgggtg ggaatattct ctgccgatgt
     2341 tttgacagga actatcatga tgattatagt aacctgcttc gtcgggtcgg tagtgaccca
     2401 gcgttatgca aaaaaaatta tcttgcaaga gactcagtct ccatcggagt ccgaaacagg
     2461 gcgtgttgat cgaattctta tacctattgc cagagagtct aatgttacag ggcttatgga
     2521 ctttgcattt ttgcttcatt ccaaaaccgg acaagagccg ctttatccat tacatgtaac
     2581 tgtggacagc agtgatgttc agcagggaat tatagaagga gagacaattc ttacaaaagc
     2641 gggtatacga gctagtgcag tacaaaaaac aacacttccc ttaaacaaga tagataccaa
     2701 tatctcttcc gcgatactta gggcgattga tgaacaacga atatctaaaa ttgttatggg
     2761 gtggaacgag tctggtaaac tgactggatc tgttttgaat actattgctg agcagattgc
     2821 aaaaaattgc agtcgtatga tctatattta caaacatata aaacctgtaa ataccatgaa
     2881 ccgaatagtg cttattgtcc ctccttacat atacaagcag tatggattca atgaaaccat
     2941 aagttctttg atcggtctaa gcggagcaat atcggcggat tggcttatta ttgctgaaga
     3001 ggagagcaat gaagatattg ctgtctattt caataaactt caaaagaaag taaatacata
     3061 taatattctt agttggaaaa atttatctgg tcatcttaag aatgttattg gagcagatga
     3121 ttttataata caacttgttc cgcggcgcgg cagtatatcc tggcggcttg attttgaaaa
     3181 aatgcctact gtgttgtgcc gttcattccc gaataataat ctgttggcat tgtatccctc
     3241 aacggtcagt gacgacagtt acgagacaat tgttagcgat gattatgatg tctccaaacc
     3301 ttcgaactta atgggagaac tgttacctgt tagtaatatt ttattcaatc ttgattatga
     3361 tgatccatca agggcaatct cccaaatagc ggatattatc ggtacagcca acagaaatca
     3421 gatatttaat acaattaata gtgtgctaaa tgaatatcct ttagaattaa cggaggatgt
     3481 tgtacttgtt catagtcgta ccgatctgct ggatgattat ttgctgttta cggctgtaag
     3541 taatcagggg tttgtttata atactttaag tgaagggggg cgataccgca ttatgatagt
     3601 gcttctatct cctttgtcaa aatccacaca aagtcattta aacgttctat cgcagatttc
     3661 acggtttgta atggtacaga agatgattga cgcgctttta atttcagaaa accgggatca
     3721 atttttaaaa catgtttcgg ttgaggatag ctaatgaatt ggcgttggca gattataccg
     3781 atggaaattg gttagtcttg tgaacaattt agcgaaaaga ttttaatggt aaagataata
     3841 catgacaata attattaaag atgactttag taaagtattt aattaatcaa ataacgagtt
     3901 gacaaattta attgtggcta tataaagata tatagccatt ggttatggtt ttgataagca
     3961 cacgttattt gagtaatcaa ctttcaaata ctgttgtata atgtgaatta attatttgtg
     4021 tctttgtatg aggttctcta tgaatttgaa tgattttata cattgtacaa acagggcgca
     4081 aagttttgag gaagtagttt ttttatttga aaaatttatt tctcagtatg gttttacaaa
     4141 ttatgtcatg ggtgatatgt cgctggattc tccggtgcaa agagcggagt cttttggaac
     4201 gttaacaaac tatagtcctg aatggcttga acattataaa aacaataact atgttgcagt
     4261 agatcccgtt ataaaaaagg ctttgatctc acatgtccct tttacatggg aggaggctaa
     4321 gtgtgaagat aaaactcaag attctctaaa tatgatgaat gaagcaatag aatttaatct
     4381 tgtcaatggt atcggattat ctgttcaccg tccctttggt agaataatag gttttggtct
     4441 agctacatcc gaaaagaatc cgcacatgga taaagacaca atggctattt tgcattcggt
     4501 ttgtgtacat ttttgtaatg tttatgagaa tttgatagga ataaacataa gtgaatatcc
     4561 tgtttgtctg actgtcagag aaaaagaggt cttgtactgg atttcccgtg gatcaacaaa
     4621 aaaaaacata gccgaacatt tcaaagttag tgaatcctgt gttaaacgcc actgtgaaaa
     4681 tatcttgcag aagttgtctg taaaaaatct tgctcatgct gtacttaagg gtgttcggag
     4741 cggattgatc tccccatact gataattatt ctccttctaa tataaattaa taattaacaa
     4801 tatttttata aaaaaccgcc tatattggcc gatcggccaa ttgacaaaaa agcccaaccg
     4861 tgctattgat agaaaaggtt taaacaaatc gtggtgttgt tgtttttaag atcaagaatc
     4921 tatctttttt tacctgtttt gattctggaa taaaattacc accagaaact ttacgtttta
     4981 caaagctttt ttgctattgt gtattttaat tataaaatag aaggtgaaaa caatgaaaat
     5041 cagtgaagaa gttaaaaagg tattgaatgc gattgataaa gcccttttgc taaaaccttt
     5101 ttttagagta cttcaaaatg gtgctttttt taaggttggt tattcttggg ttattttgag
     5161 agtaggcgga tgcttttttg ttttgttttt tttaattaag tggatcatgg tttggcctga
     5221 tgtttttaag ttatcagaaa tgccaaacat catgctttta gccatattgc tgatgctcgt
     5281 ctccttgata gtcgtttatg ctatatttaa tgcaataatg gtgaaggctg atgagatttg
     5341 tgccatatca acagaaaagg attatatagt tataattatt gtaacaaaga ttattaaact
     5401 tgctggagag atttctgcgt ttgtaatctg gtttttgggt ttaattatct ttcttttagg
     5461 ttcatcagag tttggggctg atttacttag cggaataata accttcgatt ttatggaaga
     5521 attttcggct ggtgcaggtt ctgcgtttgc ttatttaata aaatttggtt tttatggctt
     5581 cttcgcttta gccgcctcgt attttattgc ggatcttatt ggagttatgg tagatactgc
     5641 taacaatacc aaaaaaatgg atcgttaatt gcatctcttt ttgtcctaac gcattatttg
     5701 ttaattcctt cgagttaaca aataatgcgt ttgcatctct ataatagtaa tcataattaa
     5761 aatttaattg ctcaattgct ctattatcct tacaggtata tggaaggcaa aaagttataa
     5821 gtgcctgact ctttttgtgt tcacacttgg atttttaatc tgtttatatc agattaaaaa
     5881 tcgtgatact gatttcaact atattggtca tacgccggat tatcatattt atgaagaaaa
     5941 atccaaaaag atacagaagg atatattggg agaatactta tataatataa tggacaagct
     6001 ggacaagaaa attccccttt atttctaatc gaataattat tacaggataa aaacgtgaaa
     6061 aaaaatcaat ttgagtatcc agctaaaata gtatttaaaa gaggtataaa gaaaacgatc
     6121 aggaacatat caatttgcat tttgttgtgc tttgtttcat atgctggtat taatgacacc
     6181 tttgggccta cgccgttata tattcgtgta atctcttatg ctattcccct aattgttgtt
     6241 tatcttgtaa taattattat atggtcagag ttgctaaaaa agtattatgt gattgttgat
     6301 gagcaaagag taatatataa ttctctgttt aggaaaaaaa cattctattg ggtagacgtt
     6361 ataagttgga gttacaccaa acttgcttca gagaattaca ttacatttca aactcggcaa
     6421 agtgttaacg aatataataa ttctactcta ttatataaaa tatttgttaa aaacaacaat
     6481 actgcttttg gaataaatct ggatatgtgc caaaatcctg atagggctac tgttttaaag
     6541 attatagaag agattcttct ttatcatggc tggagtcgga caaattgaaa ttttataaat
     6601 aataacgata attattatca ttaagtagtt gacattattt ttttatacat tatgttgtct
     6661 ttaatagata ataataagag ctgttaaggg ttaatttttg agatttgtaa ttaataaggt
     6721 gatttcttgc cgattatggt taacttgtta gcatgtaaat ttattctttc acttgttaac
     6781 ctgctaatta attttatttc cagtttgaat agttaaaaaa gtaatagaaa gtaatagaaa
     6841 gtaatagaaa gtaatagaaa gtaatagaaa gtaataaata taggaggttt caatatggat
     6901 aatcaatcca gtatgccaaa catgccgttg ttaggggatg acttccctga actgtcggtt
     6961 cagacgactc acggaccaat gaatattcca ggtgatctta aaggctcatg gtttgtgcta
     7021 tttagccacc cggctgattt tacaccggtt tgtacaacag agtttgttgc gtttcaggca
     7081 cgttatgatg agtttgaaaa acttggctgc aagcttatag gtatgtctgt tgaccagata
     7141 ttttctcaca tcaagtgggt tcagtggatc aaggaaaatc ttgatatcga aataaagttt
     7201 cccgtagttg cggctaacga tgctgtggcg cttaaacttg gaatgcttca tccaggaaaa
     7261 ggtacaaata cggttcgtgc ggtatttatc tgtgatcctc agggtaaggt tcgcctgatt
     7321 atgtactatc cacaagagat aggccgtaac atggatgagg ttgttcgtgc tgttaaggct
     7381 cttcaaatat ctgattctca gggtgcagtg ccggctggtt ggcccgacaa tgagcttatt
     7441 aaggatcgca ttattgtgtc gcctccgaaa gacgagaaga cagcggctga acgtatgaaa
     7501 gattcgtcac ttgagtgcta cgactggtgg ttttgtcata agcccttaaa atagcttatt
     7561 tacaagttaa taaaactgca attttaacgc ccccgaatag ttaaaatttg ctgaaaagcc
     7621 actatatgat atagtggctt ttcttttggt agttatttga tttgatagtt ttaaaaaatg
     7681 cctaattatg attaatttga ttttgtctgg aaaaattcat attttgaaaa gtgttgtttc
     7741 ttattttctg tgtttctctg gaggaagtta atgcctaagg gtgaaaaatc cgcagttgac
     7801 tgtttatcaa atagtaaatt attaagtaaa aaagctccct tcgcgtatgt ttgtcatcaa
     7861 attatgcgtg ataaagatgg taaatttagt aattacaaga taatcgaagt aaatagtgtt
     7921 tttgaaacaa tttcaggttt taaaacaaaa aatataatta ataaaaatgt ctctaccctt
     7981 atttcggaaa cgagcgaaca atcgtttgat tggaaaactt tttataaaga tattctggac
     8041 cataataaaa ataaaattaa tgagcagact ttaaatcttt tagatcgctg gtacaagact
     8101 caaagttgca tgctagataa cgacactctt ttggtaatgt ttgttgatat tacgaaagag
     8161 aaggtgcagt tagatgaact tgagaacttt ttttctgtga atcttgatct gttatgtatt
     8221 gccgataccg atggaaaatt tctcaaggta aaccgtgagt gggaaaaagt tctggggtat
     8281 tctgttgatg accttgaggg tacaaggttt ctggcctttg tacatcctga tgatatagaa
     8341 aaaacaatgt cggcaataaa catgttggct tctgatgaaa aagtattaaa cttcacaaat
     8401 cgttataaag atataaaggg tgattaccat tatattgagt ggcgatctca tcctcatgga
     8461 gatttaattt atgccgcggc ccgcgatatt acccagcaaa aagaggccga gctagattta
     8521 cgcaaaagtc gtgacagatt tcagtcgttg gttcaaaaca tacccggaaa tgtttaccgt
     8581 tgcctatatg atgaagattg gactatgctt tatatcggcc gtgattttga taacattagc
     8641 ggatatcctt caatagattt tattaacaat agtaaacgtt cttttgagag tattatttat
     8701 gaagatgatc gctctatggt taggcatgaa atagatagag caatagagca aaaaaaattt
     8761 tggaatattg agtaccgtgt aaaaagaaaa gattgtggct taagttgggt tcaagagaga
     8821 gggcgagcgg tatacgacag taacggatgc atagaatatc ttgatggatt tatgttggat
     8881 attactgagc aaaaactttt taaagagcag atggttgtaa acgaaaaccg gtttcggcag
     8941 cttttcaata atatgagtag cggtgtagca atttatgaag ttgttgatag tggtaatgat
     9001 tttgttttta aggaatttaa taaggctgcg gaaactattg aaggagtata taaagcggat
     9061 gtggttggaa aaaaagtctg tgaagttttt ccttcaataa aaaattttgg gcttcttgat
     9121 gtatttaaaa gagtatggaa aactggtgtg gccgaaaacc acccggtatc gttatatcat
     9181 gatggaagaa tcgatgggtg gcgtgaaaac tttgttaccc gtttatcgga aaatgaaatt
     9241 gtatcaatat atgaggatgt gacagaaaaa aaaagagccg aaaaggcttt gattcaatcc
     9301 aaagagcagt atgagcttgc tataagtgga acaaatgatg gaatatatga ctgggatatg
     9361 cactctggtt atttgtttct gtcgaaaagg tggaaggaaa ttattggtta tagtgaagat
     9421 gaacttgaat acaatattga aacattttac tcacttattt gtgatgaaga caaacgccgt
     9481 gtagagaaac atctatcaga ctatcttgaa ggagaaattg aaaagtattc aatagagttt
     9541 cggatgaagc ataaggatgg ttctattagg tggatattgg caagaggtga agtagtccga
     9601 gatgaaaagg gtgttccata tcgaatggca ggatcgcata gcgatattac cgagcagaag
     9661 aacgcgcaga aagaattgga agaaagccgc taccgacttg aactggtaat ggatgcgggt
     9721 gaacacgggt tttgggattg gaatcttgaa actaaagatt ttttctttag cccaacatat
     9781 tttaccatgc ttggttatga taataatgaa ctaccgatga gtcttgacct atttgcaagt
     9841 cttattcatc ctgatgagag agaatatgta ttacgatcaa ttgatgaaag cataaaaaaa
     9901 ggtgagacat acgatttaga attcagacta ttgtgtaaat cgggatacta taagtgggta
     9961 tctgcaaagg gcaaaggtta tgttgacgca caaacaggta aggttgtcag agcaatcgga
    10021 attcacattg atattgatga aagaaaaaaa agtgaagaaa gaatacgaaa aagtgaagag
    10081 cagtttcgtc tgctttttga acaatctcca ttgggaatat atatagcgcg agaagatgga
    10141 accattcttg atgcaaataa aaagcttctt gaattattgg gctctccatc ggtagaggca
    10201 acaaaatcca taaacgtttt aaaatttgag cctcttgtaa aaaatggata tgccgatagc
    10261 tttataaaat gcattaaaac taaaagcatg gtcaagttag agcttgatta taaatcaaag
    10321 tggggacttg ataattatta ctcctcttac ctgattcctc ttgttgataa tgagggccgt
    10381 gcctctatcg tatacacgct tatggaaaac atcacagatc ggaaaaatat agaagaaaag
    10441 ctaaaagcaa gtgaacgtaa ttttagaact ttctttgaaa ccatggatga tatgatcttt
    10501 atcggtgata aaaagggtaa tattttttat actaacaaag ttgttgaaaa taaacttgga
    10561 tatagtcgtg aagagttggc ctcaatgcat atgctggatg ttcacccgaa tgaaaaacga
    10621 aaagaagctg aaaatatatt tgaaaggatg tttgctggtt cattagcagt ttgtcctctt
    10681 ccattacaaa aaaaggatgg agcattgatt gctgttgaaa cacggatctg gtttgggcaa
    10741 tgggatggta aagagtgtat atttggtatt tcaaaagata tatccgcaga acaggccgcg
    10801 ttgcaaaagt tcaataaact tttcaataat aatcccgcgc ttatggctgt gacatcggta
    10861 cccgacggac gatttgtgga tgtaaacgac tcatttgtaa aggttttggg atatagtaaa
    10921 aatgaagtac ttggagtatt gtcacgggat attaatattt ttggtaataa tgatactctt
    10981 gataagatcg gtagacagtt taaagaaaaa ggttcggtta aaaatcttga ggttagagtc
    11041 tgtaaaaaga atggagagct gttggatgga ttgttttcag gcgagctgat cgaaacacaa
    11101 ggacaaagct actacttgac tgtattaatt gatattaccg cacgtcgaga agcgattgag
    11161 gctttacgaa aatcggaaga aaatcagagc atactgctag ataatattca aacgcaggta
    11221 tggtatttga ctgatccggt tacttacggt gctgtaaata gtgctcatgc cgagtttagt
    11281 ggaaaaagca aggaagatct tgcttttaag aatctttatg atattttccc aaaagaaaac
    11341 gcggatatat gcagtttatc aaatgaagaa gtttttagtc aaggacacgc tgttttcact
    11401 gaggagtgga tgcctcatca cagcggagat aatcgtctgc tttccatagc caagataccg
    11461 cgactcagag aagacaagac agttgaatat gttgtttgca gtgcagaaga tattaccgaa
    11521 agaaaaattg ccgaagataa tctggttatt gcattgcaac aatcggatat acttcgggaa
    11581 agagcggaag aagccacaaa agcaaagtct cagtttttag caaatatgag ccatgaaata
    11641 agaacaccat taaatggagt aattggtttt acggagcttt tgttaaaaac tccgcttaat
    11701 gatgtacaac agcagtatgc ttacaatgcc aatacagccg ggcaaactct tcttgccata
    11761 attaatgata ttctggattt ttcaaaaatt gaggctggtc gacttgacct tgatttagtc
    11821 gagtcagata tattattatt actggatgaa tgcgctgata taataaaata tcatgcatcg
    11881 caaaaaggcc ttgaactgct gcttgatatc gagcctggca tgccgcgata cgcaatgctt
    11941 gatcctgttc gcttaaaaca gatattgatt aatctgcttg gaaatgctgt aaagtttacc
    12001 ggaagcggag aagtcgagtt aactgttaaa tttaaccgaa tagatgataa aaatggaatc
    12061 tataatttta ctgtgcgcga taccggtatt ggtataagtc gtgagcagca aaataaactt
    12121 tttaaggctt tcactcaggc cgatagtacc actacccgta aatatggcgg aacgggctta
    12181 ggacttgtaa tttccagttt gcttgtcgag aagatggggg gtaaactgac agtagaaagt
    12241 aaagagggga aggggagcat ttttcagttt gagatttcta ccgattattc cttaaacaaa
    12301 gctgcgaatg ataagatgaa tcttaaaaag atagatgtta aaaatgttct tattgttgat
    12361 gataatggcc gtaacagaat gatattaagg cataatcttg aatacttggg tataaattgt
    12421 gaagaggctt gggacggcaa gagtgcaatt gagatggtaa gagggggacg ctcgtttgat
    12481 ctttatatag tggattatct tatgcccgga atgaacggtc tggaaaccat cataaggctt
    12541 agaaaagaaa cagatgcgtt aaaaaagaca gctgtgatac tttacagttc ggcggatgaa
    12601 attaaaaaca gtgcggatct aaaaaataac ggagtgaatt attcactggt taaacctgtt
    12661 aaagcagatg accttataaa aattttacag gggatagggc aagcagattc tgtcacagaa
    12721 gaaaagctta acaacagttt tgttgatgtg gaagaacctg ttagtaaaga taagattaat
    12781 attcttgtag ccgaagatgt aattatgaat gttgaactta tgaaagctat gcttctggag
    12841 tatgttccta acgcaaatat agaagtagcg ttagatggcc acgaggcggt taatgctgta
    12901 aaaaataaat tatttgatct ggtatttatg gatgtacaga tgccgcgtct tgatggagta
    12961 gaggcaacaa aacagattag agaaaatgta gataataaaa aaattccgat tgtagcgtta
    13021 accgcaggtg ctacaaaaac agatcgcgat aaatgtcttg aatcgggtat gaattatttt
    13081 cttggtaaac cggtgaattc aacacaacta aaagaaattt tacgagctat ccttgtaagt
    13141 ggacatgatg ataaagtgtt tttggacact ggaagttttg ataaaaaaaa tctttttgat
    13201 cggcttcatg gtaacatgga aaactataat aaaatactcg aaattgctta taaacaattt
    13261 gaacaggaca tagtcgagct ggagcaggtt tatgatttag ctgattatga taaccttttg
    13321 aaaaaaattc atcgattaaa aggagcttcg ttaaacatgg gttttgttaa ccttgccgaa
    13381 cttgtaaagc agtttgaaac tgctgctaag gaagatccga ctggtgatca cagcattaaa
    13441 cagataatcg aagagtggat gctgcttaaa aagatgttag ggaaggagtt gggatatgag
    13501 tgaaaatgca aaaaaaaaga ttttattagt ggaagatgtt atgaccaacc ggtttttgtt
    13561 ggaatctttt ttaaccgatt acgactgtgt ttcagttggt aatggtgatg aaatgtatgt
    13621 tgaacttgaa aaagataggc ccgaccttat acttatggac attggactgc ctggagaaaa
    13681 cggattggag cttacaaaac agctggctcg aaatccggaa tttggtgaca tacctgtgat
    13741 atttttaact tctcactctg acaagcgtga aattttagaa gctgcaaagg caggcggcta
    13801 tgattatatc ttaaaaccgg cagatgattc tattttatca gctcgtattg agatggcttt
    13861 ggccagacac aaaaaaaaca agtaaactga gcaactatgg agagtattat ggattatcaa
    13921 gaaaaaccgt ctgttatggt cgttgatgat gaagcttcta atgtttttct tcttaagaat
    13981 atactgacag actataccgt aacatgcgct ttcagcgcgc aagagatgtt tgaaaatatt
    14041 gatgcagccg cgccaaaggt cattattctt gatgttatgc ttcctgatca aaacggattc
    14101 gatattgcca agaagctctc ttctgataag cggtacagct cgatacatat aatatttttg
    14161 actgctaaaa ccgaatcaac ggatgtagtt tccggattta aacacggcgg ttatgattat
    14221 ataaaaaaac cttttgatga gatcgagttg ttagcgcgag ttgaagctgc tatgaaaaga
    14281 gcctttgaaa gggaaaaact tgagagtgaa gtttttattg atccgctgac agaactgtat
    14341 aatcgtcgat atttaaccga ttacataaga agtgaatcat cacgggcgtt aaggtcaaaa
    14401 gaaaattttg cggttgcgat gcttgatatt gatcacttta agtctattaa tgataatctg
    14461 gggcatcctt gtggagatca tatcttaaga gagtttgcgc gaataattgc agacgaactt
    14521 cgtgaatatg atgttgccat aaggtatggt ggagaagagt ttctagttgt ttttcccgga
    14581 gagtcaaaaa taaatgcggt ttctgtttta gaacgtatac aggacaaggt gctgtcggga
    14641 agctatatct gggaagacac tccggtaagc ttcagtttta cagcaggtat tgccgatttt
    14701 atggaaattg atcacactag catgcctttt gaggatctga ttaacaaagc cgatcatcgt
    14761 ctttatcatg gaaaacatga tgggcgtaat tgtattgtta ttaatgatta gtagtatgtt
    14821 tttgctggct tcttagccac ctagattgaa aaaatagagg ttaaaattgt ttaaaattcg
    14881 tttataatag aaatactaag atattgtatc aggaaataga gaaaagagat aaaattataa
    14941 tttctgcctg gcttgttagc caggcagagg gatgtttctg agcgaaaatc agtactcgta
    15001 agggccaaca gcgaaggttc catcagaacc gggacgagga ttcccgtcta tatcggaaac
    15061 cacaccagaa attccgatgc catctgattt aagcggatcg ttttccgaag ccgaagtaag
    15121 ccgccagttc gaatcgaatg taggacttga gccggtgaag ttgttaacgc agcttgggat
    15181 atctgtttca aaactgctga tattattgta actaatacca gaatctaact gataaaattg
    15241 attattatag ggacacaaat aattattttg tataaatctg ttgtttgagc tgtatgaaaa
    15301 aatgcaataa tttaggctgt tgtttagcat gataatatta tttttaatat taactgaatc
    15361 tgggttttgg taataatcga atatcgcgtc accaccgtta gaaccaagta cataaataag
    15421 attattatac acattagcaa cactattatt tcctccagca atatataggc ctcttgaagc
    15481 attgccgcta gataattcgg atgtgataat attattgatg cactcgaaat ttttgaaatt
    15541 atcacaagaa atagcagttg ctcttgaaac atccgagtga aatgtctcgg caattatatg
    15601 gttgttgtat atttttacgg aaatatcagt atctgtcgaa tatgtaaaaa cagttgttat
    15661 attaagcggt tggcctgtcg agtcaatgtt atgagatcgt atcatttcat tattatggat
    15721 tattattccg gatcctaaag tgtttgtcgg catatttgtc gatctaaaac gtataccata
    15781 tttatatcct gcattagttg taataaccga gccagcacta ataataccgt tattttggat
    15841 agtaacttta aaatttgaaa aagtactatt acttaatggc tttatataga tgccatgatc
    15901 ataagaattt gtattttgtg ctggtccgca aatccgttca ttatcactaa tatcaatggt
    15961 tgtatctgtt atgtcgttaa gtaaagttga atttattgaa ctgatttcaa ttccggcatt
    16021 ctctttattc ggtgtattcc ctttcccggc tgcaatgtca tttctttcta taatgaatga
    16081 aaattcgtta agacttaatg attcatcatt gaaacatatt gcccaggcat ttgtgttgcc
    16141 gtcacctcct cgaataaacg agtcagtgat cgaaacggta ccgctctggg ttccgttggt
    16201 tagccttatt gcagcc
//
LOCUS       DAOTGZ010000036        16182 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_3064
            NODE_4340_length_16182_cov_4.834501, whole genome shotgun sequence.
ACCESSION   DAOTGZ010000036 DAOTGZ010000000
VERSION     DAOTGZ010000036.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868088
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 16182)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 16182)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:22:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,785
            CDSs (total)                      :: 2,751
            Genes (coding)                    :: 2,732
            CDSs (with protein)               :: 2,732
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 12 of 19
            Pseudo Genes (internal stop)      :: 5 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16182
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4340_length_16182_cov_4.834501"
                     /isolate="AMR_MDS_3064"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(247..1569)
                     /locus_tag="P5123_04015"
     CDS             complement(247..1569)
                     /locus_tag="P5123_04015"
                     /inference="COORDINATES: protein motif:HMM:NF025704.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4398 domain-containing protein"
                     /protein_id="HRX15457.1"
                     /translation="MIKKTVLHILIASLVFMLACGGAAVPIREMTEAKIAITKAQEVE
                     ASKYSRRNYEAALKYLTDAHSNVEEGKEEDARANAEEAKRLAMMAFDGAAPKMVEVSI
                     KDAQAAIERAAEANAEEFAPDEYTRAQVEVRQAELHLSGNKYSVARTSALASISSAEE
                     ARAIALSKKNLIRDSIVEVKTTLGKAKEYGADKDEPAKFSDALTGLKKSADLFKEDKL
                     KESYTELKSSKVLADELYLVSAQKASMRNINLADKQLKTAKKSAAAASHKDELAASAE
                     MLSAARSQHSDTLFEESINSSNESLRLSSIVAGSAPVVDEGPVMDMAGKHKVEKNDFL
                     RKLARRFYRDEKKWRLIYRANKDQIKNPDLIFPNQILDIPETDSLKSLPESSSDSDVD
                     SVESEVVSPDDYDTDTEDDSYEEDDVDPATMEPSAEISEEDTIEVIPE"
     gene            complement(1597..1932)
                     /locus_tag="P5123_04020"
     CDS             complement(1597..1932)
                     /locus_tag="P5123_04020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000406877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043856 - anti-sigma factor antagonist
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="STAS domain-containing protein"
                     /protein_id="HRX15458.1"
                     /translation="MEINQREINEIVLLDIAGEIDLYNAPEIKDIINKLVEEQKYNVI
                     INLEKVTYIDSSGIGALISSLSNLKKYQGGLKIINVFASVRKVFELTKLTSFFEIFDS
                     EEEALASFK"
     gene            complement(2124..3161)
                     /locus_tag="P5123_04025"
     CDS             complement(2124..3161)
                     /locus_tag="P5123_04025"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF021127.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042578 - phosphoric ester hydrolase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0045227 - capsule polysaccharide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CapA family protein"
                     /protein_id="HRX15459.1"
                     /translation="MRRTILFTFLVTVFVFSESRAVFAENFTILTMGDLLIANAVEKR
                     MDSKGKDYPFKKLRPYLEKYDIVVANLETPVTLRGNPHPSKKWTFKTRPDTAGHLKTL
                     NIHVLSLANNHLMDYGTQGLEDTVKFVKEAGFYYSGAGINLDEARKPAILNKNDSKLV
                     FLSYCERPPMDMYAKKTSAGIAPIDIDIIRSDIKRYKGHNVFVFVSLHWGIERTRYPR
                     KDQITTAHAIIDAGAEAIIGHHPHIPQGIEVYKGKPVIYSLGNAVCGFYNREYTSNIM
                     VSFHYSKGKMEKLEIIPMAGDNYKIHFQPYVERGAEARVTLAKIIEISAPFKTAISIE
                     NSRGVVRFDNP"
     gene            3324..4013
                     /locus_tag="P5123_04030"
     CDS             3324..4013
                     /locus_tag="P5123_04030"
                     /inference="COORDINATES: protein motif:HMM:TIGR00044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YggS family pyridoxal phosphate-dependent
                     enzyme"
                     /protein_id="HRX15460.1"
                     /translation="MYIIENYNRIRSHIDTIAVSCGRDPNTITLLPVSKTFPAKTVQD
                     SIDAGLKLFGENKIQEASSKMELLQGDFSFHLIGHLQSNKAKHAVKLFEMIHSVDKLS
                     TAKKIAREAIKIGKTQKILVQVNTSGEESKFGCTPGEALEIFTGLKGAEGLDVQGLMT
                     IGPLTDDLEKIRASFIMLRKLRDSLQDTLSMRLPHLSMGMSGDYKIAIEEGATILRIG
                     SAIFGTRSYPT"
     gene            4060..4845
                     /gene="proC"
                     /locus_tag="P5123_04035"
     CDS             4060..4845
                     /gene="proC"
                     /locus_tag="P5123_04035"
                     /EC_number="1.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010990394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004735 - pyrroline-5-carboxylate
                     reductase activity [Evidence IEA];
                     GO_process: GO:0006561 - proline biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyrroline-5-carboxylate reductase"
                     /protein_id="HRX15461.1"
                     /translation="MKKNTGFIGLGNMGGALIRGLSSTYGKTQMFGYDNEASKLKEFE
                     GMITACSSAQELMDKCDIIILAVKPYTVRPILEKLTSNKTSVIVSIAAGVTLETMALS
                     TSADQKIIRVMPNTPALSGEGMTVLCPNRNVSEEELTEVSSIFAKTGKTMITEEKHMD
                     AVTAMSGCGPAYFFTFIQAMADGGVKLGLSRENAVLLAAQTALGAAKMVMTSNEDPIT
                     LRNRVTSPGGSTIAAVHVLEQNGFSGIVMDAVEEAARVSSQLG"
     gene            complement(4846..6462)
                     /gene="pgi"
                     /locus_tag="P5123_04040"
     CDS             complement(4846..6462)
                     /gene="pgi"
                     /locus_tag="P5123_04040"
                     /EC_number="5.3.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004238275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004347 - glucose-6-phosphate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate isomerase"
                     /protein_id="HRX15462.1"
                     /translation="MRKDLTKSAQWKALAKHRRKIEKVSLKQLFAEDPERAKKYTIKD
                     EDIYFDYSKNLINDNTMGLLLSLAESCELSSEIENMFTGKKINETENRSVLHTALRNR
                     SNAPVMVDGVNIMEDVNRVLEKMRAFSDGIRSGERTGFTGKKFKNIVNIGIGGSDLGP
                     VMVTEALKPYVNRDLKLYFVSNIDGTHLAEVIRDIDAEETLFIVASKTFTTLETMTNA
                     HSARKWLLEKLANEESIAKHFVALSTNADAVSAFGIDTQNMFEFWDWVGGRYSLTSAI
                     GLSIMIALGYENFSSLLDGFHAMDNHFRTKPFGENIPVLMALLGVWYNNFFDAQSYAL
                     LPYDQYMHRFAAYFQQGDMESNGKSVSRNGKVVDYQTGPVIWGEPGTNGQHAFYQLIH
                     QGTKLIPADFIGFAQSHNPIGDHHIKFMSNFFAQPEALAFGKTREQLIAEGEKEELIP
                     FKIFEGNRPTNSIMAKKLTPFVLGKLIAMYEHKIFVQGVIWDIYSFDQWGVQLGKVLA
                     EKIIPELTSDEPLKHDSSTSALINYFRQNR"
     gene            complement(6455..9289)
                     /locus_tag="P5123_04045"
     CDS             complement(6455..9289)
                     /locus_tag="P5123_04045"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HRX15463.1"
                     /translation="MSETKGYKTLKQILQMSSRPECRYADFISELVGLLVKSGLCRSA
                     VIHLNQPNSNFAARYALADSESALLNNEKTDRILNLLYDNPCVTSIHEDGQDFTLVPV
                     KTAYMVYGSFILFHENDKLDSSALEFFESSAGFFALYLERLYLEPSKKTIERDLIYDD
                     NFIRVIDAIPEPLFFKGLDSRYKFCNKAFLNFVKKEKLEVIGKKSIDVLPGTQFEFHT
                     GIDAKIVRTGKSQEYECDFESSNGDIRHILIRKNFLKGSDGDVVGFVGVIRDITDLKK
                     IEEQYEDKNQALMKINSALLSTQKEIMQVNKKLSENEEKYRLLVENQREFVVKLDITG
                     QFIYSSSSFDKFVNNKSPDTEGKTFQDYLIGADKVRFQSMLRDAAVTGSETEIEMRMH
                     VGGVFHWISWSFKSIKSSARELDFIIGVGRDVTLRRKTEIELENYRSLLEAAFAQSPN
                     PLILIKYPDKKIMIMNEAAQRFFELAQDSYEDRMIEDIDFPDQVYVAGEKVDIINMLT
                     SEKIYYDSFSNTELHVKHSSGHNFFALLYSTPIYNQIGELISYLMIFPEITDLKEAEK
                     RIEEQNREIQKIQKLESIGVLAGGIAHDFNNILGAVMGNLSLARFYGGDEKITALLSK
                     AETAIGRAKELTNQLLTFSKGGAPVRKTASLNEIVRESALFSIHGSKAVIEFRLDQNL
                     KPGLIDSGQISQVIQNLVMNASESMTDGGVITVSTINVEICGDDLPLEPGEYVKVTVS
                     DHGNGVSPEIADRVFDPFFSTKNDGNGLGLTTSFNIIKKHSGYIGFNSREKGTDFFIY
                     LPVSTEVVPEIHASYEKRDKYDGKILLMDDDPEIRDVGRNLLKLIGFEVNTVNDGKEL
                     LSIVNSSKDAGIKYDLYVMDLTILGGMGGEETIGELLKIDPDARVIVSSGYANNEVLS
                     EYRKYGFCGVLEKPYTITDVKKVLKEINYA"
     gene            complement(9447..11312)
                     /gene="htpG"
                     /locus_tag="P5123_04050"
     CDS             complement(9447..11312)
                     /gene="htpG"
                     /locus_tag="P5123_04050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013252905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone HtpG"
                     /protein_id="HRX15464.1"
                     /translation="MSQHQFQTEVSQLLHLIIHSLYSHKEIFLRELVSNSSDALDKLK
                     HLTLTDDAYKQVEFDPRIDISFDEKAKKLTISDTGIGMNENDLVENLGTIANSGTKKF
                     VEQLTGDAKRDSNLIGQFGVGFYSCYMVADKVEVVTRKAGENDAYLWSSDGKTGFDIE
                     KAERDTHGTSITLHLNEEGVEYVSRWEIQNIVKKYSNHIPFPIFLHYEDTRTEGEGDD
                     AKTITEQKEEQINAASAFWKRSKSDLTDNDYKEFYKDLSHDYEDPMFWSHIHAEGTLD
                     YTTLFYIPSKAPFDLYRADYTPGVKLYVKRVFITDDEKELMPTYLRFVKGIIDSEDLP
                     LNVSREILQQNRVLAKIKTNSVKKILSEFTQYAKDSEKYEQFYKEFGRPLKEGLYQDY
                     ENRDTLLELVRFKSTKVEGLTSLADYASRMQGDQNTIYYITGENEASLRLSPLLEMYK
                     KKDIEVLILDDEIDEIVIPSVNKYKDFELKSVNRSDAADELKTDDDKKAEENLKPLIE
                     RIKKVLGDKVKDVKASSRLSDSPSCIVADTSDPTFQMQAMLKAMGQDAPSSKPILEIN
                     PGHSIIEKMQKMEDGDLFNDSVLLVFEQALVVEGLKLENPADFVKRMNDVINRAL"
     gene            complement(11538..12302)
                     /locus_tag="P5123_04055"
     CDS             complement(11538..12302)
                     /locus_tag="P5123_04055"
                     /inference="COORDINATES: protein motif:HMM:NF014826.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PHP-associated domain-containing protein"
                     /protein_id="HRX15465.1"
                     /translation="MIFKSDLHIHSCLSPCGSLDMSPLDIVKIARDNGLNLIAISDHN
                     SALNCPALKEVCDQYDDISCLFGIEVTSVEEVHNLTLFDDLEAVLDFGSFLYERLDTI
                     PNNPEAFGDQVYVNGKNEIIGEVDKYLAGATSLSVEELLVEVHNRGGLFIPAHVDRAS
                     YSLTSQLGFIPDNPYDALEFSKFYYRDQAVGSNNQSVINSEGYPHITNSDSHYPDTLG
                     MVYTEFDIEFPSINCIREALKNGRFNICTPWNKKFA"
     gene            complement(12299..12631)
                     /locus_tag="P5123_04060"
     CDS             complement(12299..12631)
                     /locus_tag="P5123_04060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013970156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRX15466.1"
                     /translation="MRVSEVAAKTGIEIVTKSFNDIEVANGYTSDLLSDVMGFAEEES
                     LLITIQAHKNTVAVASQLDLAGIVICNGRKIPEDMRLAAEQEGIAILQTEENQFVTSH
                     KIYSALRE"
     gene            complement(12618..13559)
                     /locus_tag="P5123_04065"
     CDS             complement(12618..13559)
                     /locus_tag="P5123_04065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013970157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HRX15467.1"
                     /translation="MPHRFPILSEDSADKVPSLTMEIIFRLKVKDAMMTSLWTATKKH
                     TFREIQTVMRDNEISGVPVLDDNRLVGIVSVSDIINALDSGHIEEPIEAYMSKQLIVL
                     EDDMPLSFAVSYYNKYPYRRFPVINKNKEFVGILTTKDVLTAILNEMNNEINILENQL
                     HHDKVELPNQIIREYIIKQFDFEKAGQASFKLKKLLKEKEVSRKIIRRASVAAYEMEI
                     NVAIHSNGGKITFVLEKDSITIITKDTGPGIADVAQAIEEGYSTANDWIRSLGFGAGM
                     GIPNTKRVSDEFKIESEPGKGTIVKSVIYLEETDESQ"
     gene            complement(13561..13905)
                     /locus_tag="P5123_04070"
     CDS             complement(13561..13905)
                     /locus_tag="P5123_04070"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRX15468.1"
                     /translation="MRKKVLLSDVVTAVKGRVLNNGDADYVRWVVSSGLMSDVLTTEE
                     EEILLLSSLTTTQVVRTADMVGAHAILLACGKPISSDTVSLASELGITLISCEIPVFE
                     AGHLLAPLFFGD"
     gene            complement(14245..14544)
                     /locus_tag="P5123_04075"
     CDS             complement(14245..14544)
                     /locus_tag="P5123_04075"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRX15469.1"
                     /translation="MNEINKRLGDSNWDYKIASTVIGRKKSQRNRMVALLMVIVFFSG
                     LLTYITSFNADSEYYINNQIYSVIEKSYSDNSDLYAIIETEQSLDSLIYSELASR"
     gene            complement(14544..15065)
                     /locus_tag="P5123_04080"
     CDS             complement(14544..15065)
                     /locus_tag="P5123_04080"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor"
                     /protein_id="HRX15470.1"
                     /translation="MISERDFAQIVGKTKSIVLSAVAKNLPERFHHLIDDAVQETYLR
                     AYRALVNDKFKGESELSTWLYAIARNEALRITKKSLREEQKQKKFSQKSMERIENKDV
                     KDSDDVFAQKEAYQNYLTRLPRIQKEVVDLYLRGFTEKEIAASLSIAGGTVKSRLSRA
                     RELISRMRNSEGK"
     gene            complement(15062..15538)
                     /locus_tag="P5123_04085"
     CDS             complement(15062..15538)
                     /locus_tag="P5123_04085"
                     /inference="COORDINATES: protein motif:HMM:NF019430.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Spy/CpxP family protein refolding chaperone"
                     /protein_id="HRX15471.1"
                     /translation="MIYNKVMIFFTALLLLTSCEKFPFGKDNQNSVKSERFGMSRRIR
                     LHGSRAENMDLERLCRGLDLTEEQGLEIKKINEIFSGRHLAIREVLEPLEDNLRVELR
                     RDKVDLNKVKDILKEMSEPEISRRLLMIEHRIEVEKVLSPEQLSRARRHNRQFRKR"
     gene            complement(15676..>16182)
                     /locus_tag="P5123_04090"
     CDS             complement(15676..>16182)
                     /locus_tag="P5123_04090"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GGDEF domain-containing protein"
                     /protein_id="HRX15472.1"
                     /translation="NRIQKAIFNSKYDSLTGVYTRGYFEDLCKVTMDQAYRGGLPLQI
                     VLIDLNDFKMINDTYGHSAGDNALKFFASEYIALKREADLFCRYGGDEFVALFFNCDM
                     DGVSLFFELLEETLRKNTFSAADTSLNISISWGCASVHRDGETLTELVNCADKRMYED
                     KRRRKKVI"
BASE COUNT         4811 a         3532 c         2781 g         5058 t
ORIGIN      
        1 cacctgcagc ataaccaata tatgcagttg ctgccgactg acccacactt aaaagaccct
       61 gacccaggct catggtaccg tcagcaacca gaatggcact ttcggcagcc gaaagggcaa
      121 cagctaccca cggttgacat catagtataa ttacctcatt attttggttc acaatttttt
      181 ttgagcaaac aaaaagacgc ctcaaagaga cgtctcttta tatgtttcaa atctgtataa
      241 gccaaattac tccggaataa cttctattgt atcttcttca gatatctcgg cagaaggttc
      301 catggttgca ggatcaacgt catcctcttc gtagctgtca tcttctgtat ccgtatcata
      361 atcatcagga cttacaactt cactctcaac agaatctacg tcactatctg aagatgattc
      421 aggcaacgac ttaagcgaat cagtttcagg tatatcaaga atctgattag ggaaaatcag
      481 atcaggattt tttatctgat ccttgttggc tctgtaaatc aaacgccatt ttttctcatc
      541 acgataaaaa cgacgagcaa gctttcttaa aaagtcattc ttttctactt tatgtttacc
      601 agccatatcc ataacagggc cttcatcaac aactggagca gaaccagcta ctatcgaaga
      661 aagtctcaac gattcatttg aagagttaat tgattcttcg aatagtgtat cgctatgctg
      721 tgaacgggct gccgaaagca tctctgccga agcagcaagt tcatccttat gtgaagcagc
      781 agcagctgat ttttttgccg tcttaagctg tttgtctgca aggttaatat tgcgcatcga
      841 agctttctga gcagaaacca gataaagctc atcagctaaa actttggatg atttaagttc
      901 tgtataactt tctttcagtt tatcttcttt aaaaagatca gccgattttt taagacctgt
      961 taaggcatca gaaaattttg caggttcatc cttatcagcg ccgtattctt tagcctttcc
     1021 caatgttgtt ttaacttcaa ctatagaatc acgtataagg ttctttttgg aaagagcgat
     1081 cgcacgagcc tcttcagccg acgatatcga agctaaagcc gatgttctgg caacagagta
     1141 cttatttcct gaaagatgaa gttctgcctg acgtacttca acctgggctc tggtatactc
     1201 gtctggagca aactcttcgg cattagcctc tgcagctctc tcaatagcgg cctgagcatc
     1261 ttttattgat acctcaacca tcttcggagc agcaccatca aatgccatca ttgcaagacg
     1321 ctttgcctct tcggcatttg ctctagcatc ttcttctttg ccttcttcaa cattagaatg
     1381 agcatcagtc agatacttaa gcgcagcctc gtaatttctt cgtgaatact tggatgcttc
     1441 aacttcttgt gcttttgtga ttgcaatctt ggcttcggtc atctcgcgga ttggaacagc
     1501 tgctccaccg caagcaagca taaataccaa agaggcaatc aaaatatgca gtactgtttt
     1561 ttttatcatt ggagtctcct accagttctg gtgaatttat ttgaatgacg ccagagcttc
     1621 ctcttccgaa tcaaaaatct cgaaaaacga agtcagcttt gtgagttcga ataccttgcg
     1681 aacagaagca aatacattaa ttatttttaa tcccccctga tactttttca ggttggacag
     1741 actggatatc aacgctccta ttcctgaaga gtcgatatat gttacctttt cgagattgat
     1801 tatcacattg tacttctgct cttctaccaa tttattgatg atatccttta tctccggcgc
     1861 attataaaga tctatctcac cggcaatatc cagaagaaca atctcattta tttctctctg
     1921 attaatctcc attaaaacaa ctccttaatt attgtggctc tctctataaa cgccatgcgt
     1981 tcaataatat ttccatttta tcaagctgtc aagataatta atggttaatt tgcatttcta
     2041 gcacaaattt taccagatat aactataaaa acaaaggccg taaaatgtac ggctattttg
     2101 ccttttatgt ctctaataat catctacgga ttgtcaaatc gcacaactcc cctgctattt
     2161 tctatggaga tggctgtttt gaaaggagca gaaatttcga ttatttttgc cagtgttacg
     2221 cgagcctctg ctcccctctc tacatagggc tggaaatgga tcttatagtt atccccggcc
     2281 atgggtatta tctcgagctt ttccattttt cccttagaat agtggaaaga gaccataata
     2341 ttacttgtat actccctgtt atagaagccg catacggcgt ttcccaatga atatattaca
     2401 ggcttaccct tataaacttc aattccctgt ggtatatggg gatggtgacc tattatcgcc
     2461 tctgcaccag catcaattat cgcatgagcg gtcgttatct gatcttttcg tggatatctt
     2521 gtacgctcga taccccagtg aagcgacaca aacacaaaaa cattatgccc tttgtaacgt
     2581 ttaatatcgg aacgaataat atcaatatca ataggagcta taccggcaga tgtttttttc
     2641 gcgtacatat ccattggcgg cctttcgcag tatgataaaa aaacgagttt agaatcattt
     2701 ttatttaaaa tagctggttt gcgtgcctca tcaagattta tacctgcacc gctataataa
     2761 aaaccagcct ctttaacaaa ttttaccgta tcttcaagcc cctgcgtgcc ataatccata
     2821 agatgattat ttgccaaaga caaaacatga atgttcaggg ttttcagatg ccctgccgta
     2881 tctggacggg ttttaaatgt ccatttctta gatggatgcg gatttcctct caatgtgacc
     2941 ggtgtttcaa gatttgcaac gactatatca tatttttcaa gataaggacg aagcttttta
     3001 aacggatagt ccttaccttt gctatccata cgcttttcaa cagcattagc tatcagaaga
     3061 tctcccattg ttagaattgt gaaattctca gcaaatacag ctctactttc ggaaaagaca
     3121 aatacggtta caagaaacgt aaaaagaatg gttcttcgca aaacaagcct ccaaaatact
     3181 atttgacatg ccaactttat atgtttatat aattagtgta aggttttaac gtcaaaccaa
     3241 aaccggagaa tataccacaa acatgatctt gttttatcat aaacatttcg ggcaaaacac
     3301 tattactccc ggcaggaaat acaatgtata taattgaaaa ctataaccgt atacgctctc
     3361 atattgatac aatcgccgta tcatgtggtc gtgaccccaa tacgattacc cttcttcccg
     3421 tcagcaaaac ctttccggca aaaaccgtac aagattctat cgacgcaggg cttaaattat
     3481 tcggtgaaaa caaaattcag gaggcctctt caaagatgga gcttctccaa ggtgatttca
     3541 gttttcacct tattggacat ctgcaatcta acaaggctaa acatgccgta aaactttttg
     3601 aaatgatcca ttctgttgac aaactttcaa cagcaaaaaa aatagcacgc gaagcaataa
     3661 aaatcggcaa aacacagaag atactggttc aagtaaacac ctcgggagaa gagagcaaat
     3721 ttggctgtac acctggcgaa gcactcgaaa tttttacagg tttaaaggga gcagagggtc
     3781 tggatgttca aggtttaatg actattggcc ccctaactga tgatcttgaa aaaatacgtg
     3841 cctcttttat catgttacgc aaacttagag attcacttca ggacacacta tccatgcgac
     3901 tcccccatct ttcaatgggg atgtctggag actacaagat cgcaatcgaa gagggagcaa
     3961 caatattaag aatcggatcg gctatttttg gaacaagaag ttaccctacg taatctgtta
     4021 aacatatact tgcacaaata aaatatacga gagataaaga tgaaaaaaaa cacaggattt
     4081 atcgggcttg gaaatatggg tggagccctt atacgcggac tttcttcaac atacggcaaa
     4141 acccagatgt ttggatacga taatgaagca tcaaaattaa aagaatttga gggcatgata
     4201 actgcttgta gttcggctca ggagctaatg gacaagtgtg atattatcat tttggctgta
     4261 aaaccctaca cagtgcgtcc cattctggag aaactaacct ccaacaaaac atcggttatt
     4321 gtttcaatcg ccgcgggtgt aactcttgaa accatggcac tttccacatc ggctgaccaa
     4381 aagatcatac gcgtaatgcc aaacacaccc gctctttcgg gtgaaggcat gacagtttta
     4441 tgccccaacc gtaatgtcag cgaagaagag ctaaccgagg tcagctcaat attcgctaaa
     4501 acaggcaaga ccatgataac cgaagagaaa catatggacg ctgtaactgc aatgtcggga
     4561 tgcggaccgg cctatttttt tacttttatt caggccatgg ccgacggagg tgttaaattg
     4621 ggtcttagca gggaaaacgc ggttttactt gccgcccaaa cggctcttgg ggcagccaag
     4681 atggttatga catccaatga ggatcccata acattaagaa accgtgtgac ttctccaggt
     4741 ggcagtacaa tcgctgctgt tcacgtactg gaacagaacg ggttttccgg aattgtaatg
     4801 gatgccgtcg aagaagctgc acgtgtatca tctcaattgg gctgattacc tattctgcct
     4861 aaaatagttt atcagagccg atgtagacga atcgtgttta agcggttcgt ctgatgtcag
     4921 ctctggtatg attttttcag caagaacctt gccaagctga actccccact gatcaaaaga
     4981 gtagatatcc catattactc cctgaacaaa tatcttatgc tcatacattg cgataagttt
     5041 acccaataca aacggagtaa gtttttttgc catgatcgaa tttgtggggc ggttgccttc
     5101 aaatatttta aacggtatca actcctcttt ctcaccctcg gcaattagtt gctccctggt
     5161 ttttccaaaa gccaaagctt ctggttgagc aaaaaaattg gacataaact tgatatggtg
     5221 atcacctatc gggttatgac tctgagcaaa acctataaaa tcggctggta ttaattttgt
     5281 tccctgatga atcaactgat aaaaagcgtg ctgtccgttt gtaccaggct ctccccaaat
     5341 tacagggcct gtctgataat caacaacctt gccattacgt gaaactgatt taccgtttga
     5401 ttccatatcg ccctgctgaa aataagcagc gaaacgatgc atatactgat cataaggtaa
     5461 aagcgcgtaa ctctgtgcgt caaagaaatt attataccat acgccaagaa gagccataag
     5521 aacgggaata ttctctccaa agggcttggt tctaaagtga ttatccattg catgaaaacc
     5581 atcaagaagt gaagaaaaat tttcataccc aagtgctatc attatggaaa gaccaatcgc
     5641 ggaagtcaaa gaatacctgc caccaaccca gtcccaaaac tcgaacatgt tttgggtatc
     5701 tataccaaat gccgatacgg catccgcatt ggttgaaaga gcaacaaaat gttttgctat
     5761 tgattcttcg tttgcaagtt tttctagcag ccatttgcga gcactgtggg catttgtcat
     5821 tgtctccaac gtggtgaatg ttttagaagc aacaataaaa agggtctctt cagcatctat
     5881 gtcccttatt acctctgcaa ggtgagttcc atctatgtta gaaacaaaat aaagttttaa
     5941 atcacgatta acataaggct taagagcctc ggtaaccatt actgggccca gatccgaacc
     6001 gccaatacca atattaacta tatttttgaa tttttttcct gtaaatccgg ttctttcacc
     6061 tgaccttatc ccgtcactga atgcacgcat tttctcaaga acgcgattta catcttccat
     6121 aatattaacg ccgtcaacca taaccggagc attcgagcga ttacgcaaag ctgtatgcaa
     6181 aacagatctg ttttctgttt cgttaatttt tttcccggta aacatgtttt caatttcact
     6241 tgaaagttcg caactctcgg ctagtgatag aagaagcccc atcgtattat cattaataag
     6301 gttttttgaa taatcgaaat agatatcttc gtctttaata gtgtactttt tagctcgctc
     6361 aggatcctcc gcaaaaagtt gttttaaaga gaccttttca attttacgcc ggtgtttggc
     6421 aagcgccttc cattgtgcac tttttgttaa gtctttacgc ataatttatc tccttgagta
     6481 cttttttaac atcggtaatc gtatatggtt tttcaagaac accgcaaaaa ccgtatttgc
     6541 gatactctga caatacctca ttgttagcat atccgcttga aacaatcacc cgggcatcag
     6601 ggtcaatttt aagaagctca ccaattgtct cttcaccacc cattccaccc agtatggtaa
     6661 gatccatcac atataaatcg tatttaattc ctgcatcctt cgaactattg actattgaaa
     6721 gtagctcctt gccatcattg acggtattta cctcaaatcc gatcagtttt aaaagatttc
     6781 tgccaacatc ccttatttcc ggatcgtcat ccattaacag tatttttccg tcatatttat
     6841 cacgtttttc gtaactagca tgtatttcgg gaacaacttc tgtcgaaaca ggaagatata
     6901 taaagaaatc tgttcccttt tctctggaat taaaacctat gtatccactg tgctttttga
     6961 ttatattaaa cgaagtggtc aagccaagac cattaccatc atttttagtt gaaaaaaacg
     7021 gatcaaatac acgatcagct atctcggggc taacaccatt tccatggtct gatactgtaa
     7081 ccttgacata ttcaccaggc tccaatggaa gatcatcacc acaaatttct acgttaattg
     7141 tagaaacggt tatcactcca ccgtcagtca tcgactctga agcattcata acaaggtttt
     7201 ggataacctg actaatctgt ccgctatcta ttaatccagg ttttagattc tgatcaagcc
     7261 taaactcaat aacagctttt gatccatgta ttgaaaaaag tgccgattct cgtacaatct
     7321 cgtttaacga ggcggttttc cgaaccggag ctcccccttt ggagaatgtc aaaagctggt
     7381 ttgtaagttc tttcgctcgc ccaattgctg tttcagcttt acttagcagt gcggttatct
     7441 tttcgtcacc gccataaaaa cgtgcgagcg atagattccc cattactgct cccagtatgt
     7501 tgttaaaatc atgagcgatt ccaccagcga gaacacctat tgattcaagt ttttgtattt
     7561 tttgtatttc acggttttgc tcctcaatgc gtttctccgc ctcttttaga tcagtaatct
     7621 cgggaaatat cataagatac gatatcagtt cacctatctg gttataaatt ggtgtagagt
     7681 ataacaatgc aaaaaagtta tgcccagacg aatgcttgac atgaagttcg gtatttgaaa
     7741 aagagtcgta atatattttc tctgatgtaa gcatattaat tatatcaact ttttctcccg
     7801 caacgtatac ctggtcggga aaatcgatat cttctatcat tcgatcttca taagaatctt
     7861 gggctagttc aaaaaatctt tgagccgcct cattcattat catgattttt ttatccggat
     7921 attttatcag tataagagga ttaggactct gtgcaaaggc agcctccaaa agtgatcggt
     7981 aattttccag ttctatctct gttttacgac gcagagtaac atcccgtccc acaccgataa
     8041 tgaagtcaag ctcccttgcc gaacttttta tcgatttaaa tgaccatgat atccagtgaa
     8101 atactccacc cacatgcatt ctcatctcga tctcggtttc acttcctgtt accgctgcat
     8161 cccgcaacat ggactgaaat ctgaccttat cagcgccaat tagataatcc tgaaaggttt
     8221 ttccttctgt atcgggactc ttgttattga caaacttgtc aaacgaactg ctcgaataga
     8281 taaattgacc ggttatatcc agtttaacaa caaattcacg ctggttttct acaagaagac
     8341 gatacttctc ttcgttctcg gaaagttttt tattaacttg cattatctct ttctgtgtcg
     8401 aaagcagagc cgagttaatt ttcattaatg cctgattttt atcctcatat tgctcttcga
     8461 ttttcttcaa atctgttata tcacggatca ctcctacgaa gcctacaacg tcgccatcac
     8521 tacctttaag aaaattttta cgtatcaata tatgtctaat atcaccatta ctactttcaa
     8581 aatcacattc atactcctga ctttttccgg tacgcactat ttttgcgtct attccagtat
     8641 gaaactcaaa ctgtgttcca ggcaacacat ctatactttt ttttccaata acctcaagtt
     8701 tttctttttt aacaaaattt aaaaatgcct tgttgcaaaa cttgtaacga ctgtctaaac
     8761 ctttgaaaaa aagtggctcc ggaatggcat ctataacacg tataaaatta tcatcataaa
     8821 tcaaatcacg ctctatagtc tttttcgaag gttcaagata taacctctca agatacagag
     8881 caaagaatcc agctgaagac tcaaaaaact ctaatgcaga tgaatcaagt ttatcatttt
     8941 catgaaaaag aataaacgaa ccgtaaacca tataagctgt cttaacagga accaacgtga
     9001 aatcttgacc atcttcatgg atagatgtca cgcacggatt atcatataat agatttaaaa
     9061 ttcgatcagt tttttcatta ttcaaaagag ctgattcgct atcggccaaa gcataacgag
     9121 ccgcgaaatt gctgttaggc tggttcaagt gtataaccgc agaacggcat aagccggatt
     9181 ttacgagtaa tccaacaagc tccgaaataa aatccgcata cctgcattca ggacgcgaac
     9241 tcatttgcaa tatctgcttt aatgttttat acccttttgt ttcactcatc catagttcct
     9301 gataaaatag accaatattt gcattatttc aataatatca tgtatgtaaa tatacgcaac
     9361 aattattcat ggtcagcaat aaaacagcgc tgcacatacg tacagcgcct tattaaaagc
     9421 attggcaaaa cgttttttag cctattttat aaagctctat ttataacatc attcatgcgt
     9481 tttacaaaat cggcaggatt ttcaagttta agcccttcaa caacaagagc ctgctcaaaa
     9541 acaagcaata cagaatcatt aaaaaggtcg ccatcttcca ttttctgcat cttttcaatg
     9601 atagaatgcc caggattaat ctccagtata ggcttcgaag atggagcatc ttgtcccata
     9661 gccttaagca ttgcctgcat ctgaaaagtc gggtcagatg tatctgcaac aatacacgac
     9721 ggagaatctg acagacgaga agaagccttt acatccttaa ctttatcacc gagtactttt
     9781 tttattcttt cgataagcgg tttaagattt tcttcagctt ttttatcatc atctgttttc
     9841 aattcatcag cagcatcact gcgatttaca gatttaagtt caaaatcctt gtacttattc
     9901 actgaaggta tcactatttc gtctatttca tcatcaagaa tcaatacctc gatatctttc
     9961 ttcttgtaca tttcaagtag aggggataac cttaaagatg cttcgttttc acctgtaatg
    10021 taatagatag tattctgatc accttgcatt cttgacgcgt aatcagcaag cgaagttaaa
    10081 ccttccacct ttgttgactt gaatctgacc agctctaaaa gagtgtcacg gttctcataa
    10141 tcctgataaa gcccctcttt caaaggacgt ccaaactcct tataaaattg ctcgtatttt
    10201 tcagaatcct tggcgtattg tgtaaactca gaaagaattt tttttactga attggtttta
    10261 atttttgcca aaaccctgtt ttgctgaaga atttctcttg atacattaag tggtaaatct
    10321 tctgagtcta ttattccctt aacaaaacgt aaatatgtag gcattaactc tttttcatca
    10381 tcagtaataa agacacgttt cacatagagt tttaccccgg gagtataatc ggctctgtaa
    10441 agatcgaaag gcgctttaga aggaatgtaa aaaagagtag tataatcaag ggtcccctca
    10501 gcatgaatgt ggctccaaaa catgggatcc tcataatcgt gcgacagatc cttgtaaaac
    10561 tccttataat cgttatcagt caagtcactc ttcgaacgct tccaaaatgc cgaggcagcg
    10621 ttaatctgtt cttctttttg ttctgttatg gtttttgcgt catcaccctc accttctgta
    10681 cgggtgtctt cgtagtgcaa gaagataggg aaaggtatgt ggtttgaata ttttttaaca
    10741 atattctgta tttcccagcg actgacatat tctacgcctt cttcgttaag atgaagagta
    10801 attgatgttc catgagtatc tcgttcggcc ttttcgatgt caaaacctgt tttaccgtca
    10861 ctactccata agtatgcgtc attctcccca gccttacggg taacaacctc taccttgtcg
    10921 gcaaccatat aacagctgta aaaaccgaca ccaaactgac ctataagatt agaatctctt
    10981 ttcgcatctc ctgtcagttg ttctacaaat ttctttgtac ccgaattggc aatggtacca
    11041 agattttcaa caagatcatt ttcattcatt ccgatccctg tgtcggaaat cgtaagtttt
    11101 tttgcttttt cgtcaaagga gatatcaatt cgcggatcga attctacctg cttgtatgca
    11161 tcatccgtca atgtcaaatg cttgagcttg tcaagggcat ccgaagagtt tgaaacaagc
    11221 tctcttaaaa agatctcctt atgagaatag agtgaatgaa tgatcaagtg cagtaactga
    11281 ctgacctcgg tttgaaattg atgttgtgac attttacact ccctgttgtt ttgatattat
    11341 ttatgaccaa aagcttacca tagacaaata ccaaaccaac atttttctta attgatgatc
    11401 tttgaattat ggtcacttct gttgataaac atttcagtgg atgcttacgc tattttcagt
    11461 aactggttaa aattatccac ctgaaacacg ttttcttgaa gttctcagat agtttgtttt
    11521 tttgagtagg tttttagtca agcaaatttc ttgttccatg gtgtgcaaat attaaacctg
    11581 ccatttttca aggcttctcg tatacagttt atggacggaa attcaatatc aaattcggtg
    11641 taaaccatgc caagagtatc gggatagtgc gaatctgaat tggttatatg tggatagcct
    11701 tccgagttta taacagactg attatttgaa ccaaccgcct gatccctgta ataaaacttt
    11761 gaaaattcaa gtgcgtcata gggattatca ggtataaaac caagctgaga agtgagggaa
    11821 tacgaggctc tatcgacatg agcgggtata aaaagccctc ccctgttatg tacttcaact
    11881 aacagctctt ctaccgaaag agaagttgct cctgcaagat atttatcgac ttcaccgatt
    11941 atctcgtttt ttccgttaac ataaacctga tcgccaaaag cttccggatt gttaggaatg
    12001 gtatccaatc tttcgtacaa aaaagagccg aaatccagaa ctgcttccag atcatcaaag
    12061 agagtaaggt tatgtacttc ttctaccgag gtcacttcaa taccgaaaag acagcttatg
    12121 tcatcatatt gatcgcaaac ctctttcaaa gccgggcagt taagcgcact attgtgatca
    12181 cttatcgcaa tcaaatttaa accgttatcc cttgcaattt ttactatatc aaggggtgac
    12241 atatcaagag atccgcacgg tgaaagacag ctatgtatgt gcaaatccga tttaaaaatc
    12301 attcacgaag agccgagtat atcttatgcg atgtgacaaa ctgattctct tcagtctgca
    12361 ggatagctat cccctcctgc tcggccgcaa gtcgcatatc ttctggaatc ttgcgcccgt
    12421 tacaaataac gataccggca agatcaagct gtgaagcaac ggcaacagta ttcttgtgag
    12481 cttgaatcgt tatcaacaga gactcttctt ccgcgaatcc catcacatca cttaacaggt
    12541 ccgaggtata accatttgcg acttcaatat cattgaatga ttttgtaact atttcaatac
    12601 cagtttttgc tgctacttca ctgactctca tctgtttcct ccagataaat aactgatttc
    12661 acaatggtgc ccttgcccgg ttcagactct attttaaact catcagaaac tcttttggta
    12721 ttcggtatgc ccattcctgc gccaaaccct aaagaacgga tccaatcatt agccgtagaa
    12781 tatccctctt ctatcgcctg agccacatcc gctattcccg gcccagtatc ctttgttatt
    12841 attgttattg aatctttttc cagtacaaaa gttatttttc cgccgttcga gtgaatcgca
    12901 acattgattt ccatttcata ggcagcaact gaagcgcggc gaattatctt gcgagacacc
    12961 tctttttctt taagcagttt ttttagttta aacgaagcct gccctgcctt ttcaaaatcg
    13021 aactgtttga tgatatactc gcgaataatc tggttaggca attccacttt atcatgatgc
    13081 agttggttct caagtatatt tatctcattg ttcatctcgt taagtatcgc ggtcaaaaca
    13141 tcttttgtgg tcagaatccc cacaaactct ttgtttttat ttattaccgg gaaacgtcga
    13201 tatggatatt tattataata tgatacggca aaagagagag gcatatcatc ttcaagaact
    13261 attagctgct tcgacatata tgcttcgata ggctcttcaa tatgaccaga atcaagagca
    13321 tttattatat cacttacact gactataccc acaagcctgt tatcgtctaa aacgggtact
    13381 ccagatattt cattatcacg cattacagtt tgtatttcac gaaaagtatg cttttttgtc
    13441 gcggtccaaa gactagtcat cattgcgtcc ttaaccttaa gcctgaaaat aatctccatt
    13501 gtaaggctag gtactttatc cgcgctatct tctgacaaaa taggaaatct atgtggcata
    13561 ttaatctcca aaaaaaagtg gagcaagcaa gtgccccgct tcgaataccg gaatttcaca
    13621 actaataaga gttattccca attctgatgc cagtgaaacg gtatcggaag agataggctt
    13681 accacaggcc agcaatatgg catgagcacc caccatatct gccgttctca caacctgagt
    13741 tgttgtaaga gagcttaaaa gtaaaatttc ttcttcctcg gtagttaaaa catcactcat
    13801 taaccccgac gacacaaccc agcgaacata atcggcatct ccattattta aaacacgccc
    13861 cttaacagcg gttaccacat cagacagtaa aacttttttt ctcataaacg ctcctgcgta
    13921 tttgtgagca aatttgaaaa cagattagat aattacataa tcagtaaaat catctgataa
    13981 aaaacagcat ttcctgtcca taagatattt taataacttg agtcaatcaa gaagttttta
    14041 tattataaag tcatcaagtt gtgaaataaa gcaggcacgc acatgaaatc ggtttcatta
    14101 aatagtagtg aacttcgaaa aattaccata ctacaaaagg tgtcatcgtt taactgccta
    14161 tactaaaagg attttcttgc aaactccttt ggtggaaaat taattattag aaattttaaa
    14221 taaaataata ggtattaaaa acaatcatct gctggcaagc tcgctataaa taagtgagtc
    14281 caaactttgt tcggtttcga taatagcgta taggtctgaa ttgtcgctgt aacttttttc
    14341 aattacagaa taaatttgat tattgatata atattcacta tcagcgttga acgaagtaat
    14401 atatgttaat agtccggaaa aaaaaacgat caccattaac aaagcaacca tacgattgcg
    14461 ctgacttttt tttcgcccaa ttaccgttga agcaatttta taatcccagt tgctgtcacc
    14521 aagtcgttta ttaatctcat tcattatttt ccctcactat tacgcatcct tgaaataagc
    14581 tcacgtgcac gcgaaagcct tgatttaaca gttccacccg caatagataa agatgcagca
    14641 atctcttttt ctgtaaaccc cctcaaataa aggtccacga cctctttttg aattcgaggt
    14701 agcctagtta aataattttg ataagcctct ttctgggcaa aaacatcatc tgaatcttta
    14761 acgtctttat tctctattct ctccatcgat ttttgagaaa atttcttctg tttttgctct
    14821 tccctaagag acttttttgt tattcgcaag gcctcgtttc tggcaatagc atagagccag
    14881 gttgacaatt cggactcacc tttgaacttg tcgtttacca gcgcccggta agctcgcaga
    14941 tatgtctctt ggactgcgtc gtcgataaga tgatggaatc tttccggcaa atttttcgca
    15001 acggctgaaa gaactatcga tttggttttg ccaacaatct gagcgaaatc tctctctgaa
    15061 atcatctttt tctaaactgc cgattatgtc tgcgtgccct actaagctgt tcaggactca
    15121 gaactttttc cacttcaata cgatgttcta tcatcaacaa ccggcgacta atttcaggtt
    15181 cggacatttc ttttaaaata tctttaacct tattcaagtc aactttatct cgacgcagtt
    15241 caaccctaag attatcttca agcggttcaa gcacttctct gatagctaaa tgacggccgg
    15301 aaaaaatttc atttattttt tttatttcta aaccctgctc ttcggtcaaa tccaatcccc
    15361 tgcaaagtct ttccaggtcc atattttctg ctctactgcc atgaagccga atgcgtctag
    15421 acataccgaa acgctcactt ttaacagaat tttggttgtc ttttccaaac ggaaattttt
    15481 cacaggaagt caacaaaaga agagcagtaa aaaaaatcat aactttatta taaatcatat
    15541 tttactccat atttacaata tataacttag acctgttttt attcattttg gttccgtttt
    15601 tattgtcagt taagctatat cttcagcgcc tctttgcatt tttaaagtgg tactgaacga
    15661 gggacaacga gataatcaga taactttttt tcgcctacgt ttatcttcgt acattcgctt
    15721 atctgcacaa tttacaagtt cggtcaaggt ttccccgtcc cggtgcaccg acgcgcatcc
    15781 ccagcttatc gagatattaa gactggtatc ggccgcagaa aaagtgttct ttctcagggt
    15841 ctcttctaaa agttcaaaaa agagacttac tccgtccata tcacagttaa aaaatagtgc
    15901 cacaaattca tctcctccat agcggcaaaa gagatcggct tcccgtttta gggctatata
    15961 ttccgaggca aaaaacttga gggcattatc acccgccgag tggccatagg tatcgttaat
    16021 cattttaaaa tcattcaaat ctattagcac tatttgcaag ggaagccccc cacggtaagc
    16081 ctgatccatt gtgaccttac aaagatcttc aaaataacct cgtgtataaa caccagtcag
    16141 actatcatat ttggaattaa atatcgcttt ctgtattcta tt
//
LOCUS       DAOTGZ010000037        16021 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_3064
            NODE_4399_length_16021_cov_5.731367, whole genome shotgun sequence.
ACCESSION   DAOTGZ010000037 DAOTGZ010000000
VERSION     DAOTGZ010000037.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868088
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 16021)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 16021)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:22:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,785
            CDSs (total)                      :: 2,751
            Genes (coding)                    :: 2,732
            CDSs (with protein)               :: 2,732
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 12 of 19
            Pseudo Genes (internal stop)      :: 5 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16021
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4399_length_16021_cov_5.731367"
                     /isolate="AMR_MDS_3064"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..574)
                     /gene="trmD"
                     /locus_tag="P5123_04095"
     CDS             complement(<1..574)
                     /gene="trmD"
                     /locus_tag="P5123_04095"
                     /EC_number="2.1.1.228"
                     /inference="COORDINATES: protein motif:HMM:NF000648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0052906 - tRNA
                     (guanine(37)-N(1))-methyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(37)-N1)-methyltransferase TrmD"
                     /protein_id="HRX15473.1"
                     /translation="MKTLNILTLFPEFFRSPFETGLLSKFLQKGDLLLNLVDIREFSD
                     DSFKRCDDYPYGGGSGMVLKPEPLFKALDSLDNRGTVILTSPSGEPLNQEKVKEFAGL
                     EDITIICGHYEGVDQRVVDEYVDFEISVGDYVLSGGEFAALIITDAVVRYLPGFMSNP
                     ESLVEESFEGSLLEYPHYTRPENFREHQVPS"
     gene            complement(571..1095)
                     /gene="rimM"
                     /locus_tag="P5123_04100"
     CDS             complement(571..1095)
                     /gene="rimM"
                     /locus_tag="P5123_04100"
                     /inference="COORDINATES: protein motif:HMM:TIGR02273.1"
                     /note="Essential for efficient processing of 16S rRNA;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome maturation factor RimM"
                     /protein_id="HRX15474.1"
                     /translation="MSDSYYTVAKVSSPFGLDGGLKLSLITDFPGRFVSGRRLFVDIN
                     GFYREVIIKSFTVTSGKHGTMFLEGVCTRADAESLRGYNLVIPESEVRDVGCDLADDV
                     FQYCDLMGCEVLYKGSSFGTVIDITEGGGGELLIIESKGREYMIPFVSEMVDTSAVRD
                     HKITISPAEGLLEF"
     gene            complement(1092..1328)
                     /locus_tag="P5123_04105"
     CDS             complement(1092..1328)
                     /locus_tag="P5123_04105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010257846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="KH domain-containing protein"
                     /protein_id="HRX15475.1"
                     /translation="MDYKELVDFLVKSLVDNPDDVEINQIEGEKSTILELSVAGDDIG
                     KVIGKHGRIARAIRTVLNAASAKSGKRVVLEILD"
     gene            complement(1339..1602)
                     /gene="rpsP"
                     /locus_tag="P5123_04110"
     CDS             complement(1339..1602)
                     /gene="rpsP"
                     /locus_tag="P5123_04110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006001995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S16"
                     /protein_id="HRX15476.1"
                     /translation="MVKIRLQRVGTKKRPFYRLVAIDHRKRRDGAVLESLGQYQPVIK
                     GEQLTLDEERVLKWLNVGAQPTDTVLDLLKKQGLWKKFSSASK"
     gene            complement(1699..2706)
                     /locus_tag="P5123_04115"
     CDS             complement(1699..2706)
                     /locus_tag="P5123_04115"
                     /inference="COORDINATES: protein motif:HMM:NF015734.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PASTA domain-containing protein"
                     /protein_id="HRX15477.1"
                     /translation="MEDNQKKSGFFMRNRKYFLLMVVYVTLFMIVSTFMIVKLNQPAG
                     KVRVPDVKGKIFNNVYNSLISRELKPEISFFDAQDVDSGIVLKQHPAPGSIVPRESRV
                     KLVISRNNQKVEVPDVTGGPLIIAKNKLSTLHIGEKKVSLLPGVVSYIPSSEHEENTV
                     IAQSPRAGEKVAPHSRINLLVSTGVLGRDSKMPQLSGQYIDLCYDLLLSLGVSVRQEI
                     VPADSVEDSGRILKQSIEPGSDVSKGEEIVLTVAYFERENRYYQSYEKVSFSIPSDSK
                     DVLYEAWVEDEEHKRIRFSKVCKANEKITFIFHRTGNARIEIVKDKVKEKIITIDVDS
                     F"
     gene            3086..4294
                     /locus_tag="P5123_04120"
     CDS             3086..4294
                     /locus_tag="P5123_04120"
                     /inference="COORDINATES: protein motif:HMM:NF018884.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016791 - phosphatase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SpoIIE family protein phosphatase"
                     /protein_id="HRX15478.1"
                     /translation="MDNRDLLKSVPVFYGINDEDEDLILPLTRQLQYKKGSIIINEGS
                     EGSSMYILIEGTVKVTKAENKDSEILIATLEAGSYFGELSLVDDMPRSANVIADDDCS
                     LLHLKKRDLDSLLENNLKIANIFYKNCLTETFSRFRKAIQNFTFSQHILREKSETLDE
                     INRDLSLAKEVQSYFINAGTNSPDKSGIKYSYIYSPCQEIGGDFLNVVPVDGEYINII
                     IADVVGHGITAAMATGVLKSAFSIFSEQFSNEPAKLMTGINKHYFEIMSRFYATCYYA
                     NVDIRNMTISFAKGGHHHPLFWKESKGQFIDIESSGIGIGIMETAHYKDVTYPIEKGD
                     KILFFTDGILEQHNKQRQMFGEEKLHAVFKHAILEDVPDKLQYLQQEFNAFCDGSEIE
                     DDITLLMLEI"
     gene            4420..4902
                     /locus_tag="P5123_04125"
     CDS             4420..4902
                     /locus_tag="P5123_04125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000956229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis protein CheD"
                     /protein_id="HRX15479.1"
                     /translation="MSIELVNVGVAQLKIASSPVILRTILGSCVGICIYDRNKKMGGL
                     AHVLLSKATNDSQPEKFAETAIPALIEQLLRQGCKRDFMSAKIAGGASMFKFASQTPL
                     GLIGENNISMTKKILEEKKIPIVGEDTGGSVGRVIDFFLEDGRLKVKAGGHEEYYYKV
                     "
     gene            4923..5783
                     /locus_tag="P5123_04130"
     CDS             4923..5783
                     /locus_tag="P5123_04130"
                     /inference="COORDINATES: protein motif:HMM:NF020252.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HDOD domain-containing protein"
                     /protein_id="HRX15480.1"
                     /translation="MHEHIKERIKTVIANLNALPSMPEVVTRIINMVNDPNVDFKLVA
                     EEISRDQAITTNILKIANSAYFSKGKEIASVEKALVTLGMKEVKDIVVVAATKQVLDK
                     PVIGYDLAKGELWKHNVAVAMLAKKIAIDKKKKNLADLVFTGGIIHDVGKTVLAIYVA
                     STFKDLIQSTVDNQITFAQAEQLLLGFDHQEIGQIILKKWQFPESLQNIVRYHHDADD
                     APQSDMEAVAIVHVANTICQMAGIGIGGDGLYYELSGKAIEATGLDDTELQHYYGITP
                     ELLKMAKQIM"
     gene            complement(5785..6960)
                     /locus_tag="P5123_04135"
     CDS             complement(5785..6960)
                     /locus_tag="P5123_04135"
                     /EC_number="2.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014810852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HRX15481.1"
                     /translation="MPIADDIKESINKSSFIRKMFEEGIELKRKLGEENVFDFTIGNP
                     DLNPPDAYYKIAGDIALDRRKNIHGYMPNAGYDNVRKAMAEKASREHACEVNYQNIVM
                     TVGAAGGLNVLFRALLNPGDEIVVPKPYFAEYGAYAGNHRAVLKPVNSGSDFSLDVKA
                     VEDALTDKTKIVLINSPHNPTGRVYDEESLKNLAQMLKKQKNVGRHIYLVADEPYREI
                     TYDGIVVPPVLSTYEESFVVTSFSKTLSLPGERIGYIAQNPKMQDSALIMNALILCNR
                     ILGFVNAPAFIQRVVGEIVDEKVDVAIYDSRRKIFVKGLEDAGLEYAKPEGAFYLFVK
                     SPLDNDIDFVDHLKKYGILSVPGSGFGGAGYIRFAYCVDEDIIKRAMPLLKKAVSEL"
     gene            complement(7196..7858)
                     /locus_tag="P5123_04140"
     CDS             complement(7196..7858)
                     /locus_tag="P5123_04140"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRX15482.1"
                     /translation="MKKIFLILLVVSCARQVEINQKIEPEKPKETGAVCIRLSLPLKN
                     IARFHDPDELYIYSETDVSGELIKSDYHVDDRFYFFELVPGRYHIAVLEYSVNDADVR
                     KGYYLLTKRSSVESAFSVISNKLYYPGELSGIFLRYTSANSDIQADRYLEYFIPESAG
                     GAFYLSSKGKDTGHYKILSNTILFYPFDDNTVSIIKRDITKLSDNHKIKNYHVVTSLN
                     RQ"
     gene            complement(7859..8842)
                     /locus_tag="P5123_04145"
     CDS             complement(7859..8842)
                     /locus_tag="P5123_04145"
                     /inference="COORDINATES: protein motif:HMM:NF013862.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="patatin-like phospholipase family protein"
                     /protein_id="HRX15483.1"
                     /translation="MFDFFSKMSRGKIGVALSSGGAKGLAHINVLEFLDEHDVKIDYI
                     AGSSIGALVGAVYYSGTLSQFKNEFGSFSWRDIVHLVDPVFPKSGIIAGKKIMKYLER
                     FIDPDINIEELPGKLAIVATDYFTGNPVIMREGNLLKAVRGSISIPGVFVPYDYDGRI
                     LIDGGVANPIPVDVVKKMGAKRVVAVNLHPSAKVIHGKKKLKKLKNIAFRRKKRTMGN
                     RIASHIKINASGWFSFLSSYLKLDNGNEPNEDPKGYEMPSIIDVFMQSMEVMEYMNTF
                     ALLRYNKPSVLIEPAVREINGLDFDSGKEVLRIGQQAVEENRKKLLRKIVR"
     gene            complement(8884..9777)
                     /locus_tag="P5123_04150"
     CDS             complement(8884..9777)
                     /locus_tag="P5123_04150"
                     /inference="COORDINATES: protein motif:HMM:NF016915.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HRX15484.1"
                     /translation="MYRHYRTVLIILLCMNILSCSRSVKLQSTCCQLKYPILFVHGIA
                     LPDDMKLVPYWGSIPDHLRNRGATVFLSNHNAWAEHKVAAQFLKDRVEQIIAQTGAQK
                     VNIIAHSKGGIESRYMISRLDMGDKVASLTTISTPHRGSMVADYLFEKIDSRQMGGKF
                     IITLASVVGVVMGDSRPDPDSSGRSLTKEYMESFNENVPDMPQVYYQSYISLITEEYP
                     SLFWQKLYELHKNLDGPNDGLVSEQSAMWGVYKGVVKVGEVSQINHGDIVDLYQFSGR
                     RVPDLHLFYVDMVHDLKDMGF"
     gene            complement(9779..11635)
                     /locus_tag="P5123_04155"
     CDS             complement(9779..11635)
                     /locus_tag="P5123_04155"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRX15485.1"
                     /translation="MTGSVNVHSRGSLLMHNINYSELDSFYESRFCLCTGENDFETIS
                     FFLAEAGFQYFDYISPEKVTSDFLVKNRFDIIIFQVAGEDHFLLLNEFRKSNTDAALI
                     LVSDSAVPTMLQLTLKVETVLSLSFDPQDIIKAVKKALGSKNNFEIGMQFFDALFGMQ
                     MCTHRDLHQRTFEHAIRTTQIYGKFLLFLMETEVIKLTSWTLKNCLMASLVHDIGKLL
                     VMQGILYKEGKLTELEYQQIKRHPWHSVTALLGGQDIEFFAGDGPIETVSGYNDKNLS
                     EKTKQWVFKVIDNDEDVYKDLDNFFESLYRKPFIHSLNKDLLYIVFRHHDSIEKSYHN
                     YDDLQLFSKIIDRDLDEKLNEKSYLDVVTNALSLCDFYDALLDKKRDYRKRPFNRSFV
                     LYFIYLETRNGRFFPFLAEEFVKYIIRNEISSEENVFCYNNDPEITLQVIKDIDSLFR
                     IAPGQEHEFNGFAIEKKELFHEYSRTFDENLLVKTNSLWVDYYSVKHSEIVNRFYNSL
                     KEANLVSKEIKDFTVQEVKVFDMLFNFYRSYPSSIKQKKLIDYFVDSVIDRKVTQNTK
                     KRIIEHLQSEEIDTVTDIKKMLIRSGYDRLNLFEVFKDYNEELLLNEFNQGL"
     gene            complement(11739..12107)
                     /locus_tag="P5123_04160"
     CDS             complement(11739..12107)
                     /locus_tag="P5123_04160"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HRX15486.1"
                     /translation="MSRIMIVEDEVILTMAIQQTLESAGFEVTPPVLSGEEAISRVQS
                     DKPDLIVMDVTLQGELDGIETTELILKKHDIPVIITTAHSDPSMTDRISKCGSRGFLL
                     KPVNFKEMVGLIRDILDNKE"
     gene            complement(12150..13073)
                     /gene="trxB"
                     /locus_tag="P5123_04165"
     CDS             complement(12150..13073)
                     /gene="trxB"
                     /locus_tag="P5123_04165"
                     /EC_number="1.8.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012995873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004791 - thioredoxin-disulfide reductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin-disulfide reductase"
                     /protein_id="HRX15487.1"
                     /translation="MNDLIILGAGPGGMSAAIYAVRSGLKTLVIEKFMPGGQVLNTAD
                     VENYPGFEDPITGYELVTAMEKQVRRLGAEIITADIQSVERDEKEDKMVCKDASGNVY
                     EARAVIAATGSRYKRLGVPGEKEYTGKGVSYCATCDGAFFRDKVTAVVGGGNTALDEA
                     LFLTRFASKVYLIHRRDKFRASQILVNRVMEHPKIEIVFNSIVTSVNGENKVNSATLK
                     DTVDGSERDLTLDGVFIFVGYNPVTEMLPDSIKDEWKAIKTDINMGTSIPGLYAAGDI
                     RATSRKQIITAAADGATAAMAAYEYITHEEL"
     gene            13256..14500
                     /gene="icd"
                     /locus_tag="P5123_04170"
     CDS             13256..14500
                     /gene="icd"
                     /locus_tag="P5123_04170"
                     /EC_number="1.1.1.42"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017250757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004450 - isocitrate dehydrogenase (NADP+)
                     activity [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isocitrate dehydrogenase (NADP(+))"
                     /protein_id="HRX15488.1"
                     /translation="MFENISTRRAIMGEKIKLEKGIITAPDNPVVLFIEGDGIGPDIW
                     NATRIVIDAAVQKAYNGSRQIDWKEILAGEKSFNKTGEWLPDETVNLIKEYKVAIKGP
                     LTTPVGGGIRSINVALRQVLKLFACVRPVRYFSGVPSVLKNPERTDMVIFRENIEDVY
                     SGIEWQEGTDDAKKVIDFINNQMGKNIAADSGIGIKPISKTNSQNLIRSAIQYAIEND
                     KKSVTLMHKGNIMKFTEGAFKQWGYDLVKAEFSDTVITEDELWDKYDGKQPEGKVVVK
                     DRIADAIFQQVLLRPTDYDVIATPNLNGDYISDALAAQVGGLGIAPGANIGASEAVFE
                     ATHGTAPKYAGQDKVNPGSLILSGEMMLRFMKWSEAADLVVSAMEKTISQKKVTYDLE
                     RQMEGATLLKCSEFANAIVSNM"
     gene            complement(14549..15970)
                     /locus_tag="P5123_04175"
     CDS             complement(14549..15970)
                     /locus_tag="P5123_04175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013265238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HRX15489.1"
                     /translation="MKVKLILPSLTEAGSAFWRPIKYSLFPPLGLATLAAFLDKDDEI
                     DLQDQHVEKLNLDDSPDLVIIQVYITNAKRAYSIAKHYRDKGVFVILGGLHVTALPDE
                     AEEYADSIFIGPGEQTFPEFLNDFKSKTVKKRYYSGNGRTIQNLPPVRRDLIKREKYL
                     VPNSLVISRGCPHHCDFCYKDSFFKGGISFYTHRVDEILTEISRLPGKHLYFLDDHLL
                     GNPKLVSELFEGMRGMNRVFQGAATIKSVIEGDLIAKAAKAGLRSLFLGFETFSVENL
                     KQSNKYQNLKQNYEYAVKRLHDLGIMINGSFVYGFDNDASDVFKRTVDWGIQNAVTTA
                     TYHILTPYPGTRLYNSMFEGGRIITNNWDMYDTRHVVFKTRGLSARELENGYQWSYKE
                     FYKWNNILKASFSHDTIKHKMKHLLYAGGWKKFEEAWKVIINCGGLKKMLPLLELLLC
                     ETSGSRTVKEDKSVQNPAVMSNQ"
BASE COUNT         5003 a         3514 c         2818 g         4686 t
ORIGIN      
        1 ccgatggaac ctggtgttcc ctgaaatttt ccggtctagt ataatgagga tactccaata
       61 aggaaccttc aaaagactcc tcgaccagcg actcgggatt agacataaac cccggcaaat
      121 aacggacaac agcgtctgtt ataatcaacg cagcaaactc cccgccggac aacacataat
      181 ctccaacaga gatctcaaaa tcaacgtatt cgtccactac cctttggtct acaccttcat
      241 aatggccaca gataatggta atatcttcta aaccggcaaa ctcttttacc ttttcctgat
      301 tcaaaggttc acccgaaggt gaagttaaaa taactgtgcc tctgttatcg agagaatcca
      361 acgctttaaa aagtggctca ggtttaagaa ccattccact tccaccaccg tatggataat
      421 catcacatct tttaaatgaa tcatccgaaa actcccggat atcaaccaaa ttcagtaaca
      481 ggtcaccttt ttgcaaaaac tttgacaaaa ggccagtttc aaatggactc ctgaagaatt
      541 cgggaaaaag agtcaaaata ttcagagttt tcaaaattcc aataatccct ctgccggact
      601 tattgtgatt ttatgatcac gaaccgcgga tgtatctacc atttcagaca cgaagggaat
      661 catatactcg cgacctttgc tctcaataat aagcagctcg ccgccacctc cttcagtaat
      721 atcaataact gtaccgaaag aagatccctt gtataaaacc tcacagccca tcaagtcgca
      781 atactgaaaa acatcatcag ccagatcaca accaacgtca cgaacctctg actccggaat
      841 aacaagatta tatccgcgta acgactccgc gtccgctcgg gtacaaacac cctctaaaaa
      901 cattgtacca tgcttaccgg atgtaaccgt aaaagattta ataatcactt cccggtaaaa
      961 accattaata tcaacaaaca atcgtcgccc agacacaaaa cgtccgggaa aatccgtaat
     1021 caaagacagc ttcaatccgc catcaagtcc gaaaggcgaa gacaccttgg caactgtata
     1081 gtaactgtca ctcaatccaa tatttccaac actacccgtt ttccggattt tgcagaagct
     1141 gcatttaaaa cagtgcgtat agcacgagct atacggccat gcttaccgat aactttacca
     1201 atatcatcgc ccgctacact cagctcaagt atggttgact tttcaccttc gatctgatta
     1261 atttcgacat catcaggatt atcaacaagc gacttaacta aaaaatctac aagttcttta
     1321 tagtccactc atccctcctt attttgaagc agatgaaaac tttttccaaa gaccttgttt
     1381 cttaagaaga tcaagaactg tgtcggtagg ttgagctcct acatttaacc acttaagcac
     1441 cctttcttca tcgagtgtaa gctgctcacc tttaataaca ggctgatact gacctagact
     1501 ttcaagaacc gcgccatcgc gtcgttttct atgatcaata gcaactaaac ggtaaaaagg
     1561 tctttttttt gtacctacac gctgcaaccg aatctttacc aattgaattt cctccaaata
     1621 tagttagagg acgattattg agaatcgccc ttttttgtca agattaatat ccgaacattt
     1681 taagccatgc cgctcatcct agaaagaatc gacatcgata gtaattattt tttccttcac
     1741 cttgtcttta actatctcta tgcgggcatt accggttcta tgaaaaataa atgtaatttt
     1801 ttcgtttgct ttacaaactt tggaaaatct tattcgctta tgctcttcat cctcaaccca
     1861 cgcctcgtaa agaacatcct tggagtcgct cggtatcgaa aaagagacct tctcgtatga
     1921 ctgataataa cgattttctc gctcaaaata ggcaactgtt aaaactatct cttcaccttt
     1981 tgatacatca gagcccggct ctattgactg tttcaatata cgacccgaat cttccaccga
     2041 atctgcaggt actatctctt gacgaacgga aactccaaga gaaagaagca aatcataaca
     2101 aagatctata tattgacctg aaagctgtgg cattttcgaa tcacgcccca gaacacctgt
     2161 cgaaacaaga aggtttatcc tggaatgcgg agccactttt tcacccgcac gaggactttg
     2221 cgctataacc gtattttctt catgttctga tgagggaata tacgaaacga ctcccggcaa
     2281 aagagatact tttttttcac caatatgtaa agtagacagt ttgtttttag caataatcag
     2341 aggcccaccc gtaacgtcag gaacttctac cttttggttg ttgcgactga taaccagttt
     2401 caccctgctt tctcgcggaa caatgcttcc aggagctgga tgttgtttta acacaatgcc
     2461 gctgtctacg tcctgagcat cgaaaaaact tatttcgggc ttcaattcac gacttattaa
     2521 tgagttatag acattattaa aaattttgcc tttaacatcg ggcactctaa cctttccggc
     2581 aggttgatta agctttacaa tcatgaaagt tgaaacgatc atgaaaagag tcacataaac
     2641 aaccatcaac aagaagtatt ttctgttacg cataaaaaaa ccggattttt tttgattatc
     2701 ttccatgaaa tcctcaacac aacctatatt tataaactaa tcgcctgtct cattatcagc
     2761 gtctctgcta aaaggaactg ctgaccacct ggaatacgta gaccgaaact gatcagacag
     2821 ctggtatatc aacaatggct cactttaccg tcaagtatta attacagaat gaaaacggag
     2881 ctttcaaccg cccagcctta ctttttcgag ctcatttaaa gaattatact ttacatacac
     2941 atagtctaat catattttat aataagattt ttggcgagtt ttttgatttt gttgtcaaaa
     3001 ctggcaaata agctgccaaa caccgaagtg gctctatttc aaacaacatg atagggtttt
     3061 ctgtaactta atatgtgggg ttaaaatgga caacagggat cttttaaaaa gcgtacctgt
     3121 tttttacggc attaatgatg aagacgaaga tctgatttta ccacttacgc ggcaacttca
     3181 gtacaaaaag gggtccataa taatcaatga aggttccgaa ggaagttcaa tgtatattct
     3241 cattgaagga accgttaagg tgaccaaagc cgaaaacaaa gattccgaaa ttcttatcgc
     3301 caccctagag gccggctcct atttcggaga gctctctctt gttgatgaca tgcctcgctc
     3361 agcgaacgta atagcagatg acgactgttc cctgctccat ctaaagaaaa gggatcttga
     3421 cagtcttctg gaaaacaatc ttaaaattgc caatatcttt tataaaaact gcctaaccga
     3481 aactttttct cgcttccgaa aggctataca gaatttcacc ttttctcagc acatattacg
     3541 tgaaaaatcg gaaactcttg atgaaatcaa cagggatctt tctcttgcga aagaggttca
     3601 gagttatttt atcaatgcag gaacaaacag tcctgataaa tcgggcatta aatactccta
     3661 tatatacagt ccttgtcaag aaataggcgg tgactttttg aacgttgttc cggtcgatgg
     3721 cgaatatata aatatcatca ttgccgatgt agtagggcat ggcataactg cggcgatggc
     3781 aaccggagtt ttgaaaagcg cattttcaat attttctgaa cagttttcta acgaacctgc
     3841 aaaactgatg accggaataa acaaacacta ttttgaaata atgtcacgtt tttatgcaac
     3901 ctgttactac gccaacgttg atatcagaaa catgacgatt tcattcgcca aaggcggaca
     3961 ccaccaccct ctgttctgga aagagagcaa agggcaattt attgatattg aatcttctgg
     4021 aattggcatt ggaataatgg aaactgccca ctataaagat gtaacttatc ctatcgaaaa
     4081 gggtgataaa attcttttct ttacagatgg aattttagaa caacataaca agcagcgaca
     4141 gatgttcggg gaagaaaaac ttcacgcggt ttttaaacac gcaatacttg aagatgtacc
     4201 ggacaaacta caatatttac aacaggagtt taatgccttc tgtgacggtt cagaaattga
     4261 agatgacata actctgctta tgcttgaaat ttaaaagcag gagttttagt aattcataaa
     4321 tattgacatg ctaattattt tttgtatatt acaaaaggga gttttttcag gtttccacaa
     4381 gatctgatta tcacatattc atcacaaaga gatatcgaaa tgagcataga acttgtaaac
     4441 gtcggagtag cacagttaaa aatagcatct tctcctgtaa tactcagaac cattctgggc
     4501 agttgcgttg gaatatgcat ttatgaccgc aacaagaaga tgggcggcct tgcccatgtg
     4561 cttctttcaa aagctacaaa cgactcccaa ccagagaaat ttgccgaaac agcgatacca
     4621 gccttaattg aacaacttct gcgacaaggt tgcaagcgtg attttatgtc cgccaagatt
     4681 gccggaggag cttcaatgtt taaattcgcg tcacaaaccc ccttgggtct tattggcgaa
     4741 aacaatatct caatgacaaa aaaaatactt gaagagaaaa aaatcccaat tgtaggcgaa
     4801 gacacggggg gatctgttgg gcgtgtgatc gatttttttc tggaagatgg ccgtttaaag
     4861 gttaaagcag gcggccatga agagtattac tacaaagtct gatacaactg gcggagagtg
     4921 tgatgcacga gcatattaaa gaaagaataa aaacagtaat tgccaacctt aacgcgcttc
     4981 catcaatgcc ggaggttgtc actcgcatta ttaacatggt aaacgatccg aacgtcgact
     5041 ttaagcttgt cgccgaagag atatcgcgcg accaggctat aacaacaaat attctaaaaa
     5101 tcgcaaactc agcatatttt agcaagggca aagagatcgc ttctgtagaa aaagctcttg
     5161 taaccttggg tatgaaagag gtcaaggata tcgttgtagt cgccgccaca aagcaggttt
     5221 tggataaacc tgttatcgga tacgatcttg cgaaaggcga gctttggaaa cacaatgttg
     5281 cagtagcaat gttggcaaaa aaaattgcta tcgacaagaa aaagaaaaac ctagcagatc
     5341 ttgtctttac cggtggcatt atacacgatg ttgggaaaac cgttctggcg atatatgttg
     5401 caagcacctt taaagacctg atacagtcaa ccgttgacaa ccagataacc tttgcccagg
     5461 ccgaacagct attgctgggt tttgatcatc aagaaattgg ccaaataata ctaaagaaat
     5521 ggcagtttcc cgagtcactt caaaatattg ttcgctatca tcacgatgca gatgatgcac
     5581 cgcaatcgga tatggaagct gtagccattg tacatgtagc aaatactatt tgccagatgg
     5641 caggaatagg aatcgggggc gatggcctct attatgaact atccggtaaa gcaattgagg
     5701 caaccgggct agatgacact gaacttcaac actattatgg catcacgccc gaattactca
     5761 aaatggccaa acagataatg taaattacag ttcggataca gcttttttaa gtagcggcat
     5821 ggctctttta atgatatcct catccacaca ataagcaaac cttatgtatc cggcgccgcc
     5881 aaatccacta ccgggcaccg aaagaatacc atattttttc aagtgatcaa caaaatcgat
     5941 atcattatcc aaagggcttt ttacaaaaag ataaaatgcg ccttcgggtt tcgcatattc
     6001 aagaccggca tcttctagac ccttcacgaa tatctttctt cttgaatcgt atatcgctac
     6061 atccactttt tcatcgacaa tttcaccaac tactctttgt ataaatgctg gtgcgttaac
     6121 gaaaccaagt attctgttgc ataaaataag cgcattcatt attaacgcgg aatcttgcat
     6181 cttgggattt tgcgctatgt aaccaattcg ttctcccgga agtgataatg tttttgaaaa
     6241 tgaagtaaca acaaaagatt cttcataagt tgaaagtact ggtggcacaa ctattccatc
     6301 ataagttatt tcacgataag gctcgtcggc cacaagatat atgtgacgac cgacattttt
     6361 ttgcttttta agcatttgtg ccaagttctt caatgactct tcatcataaa cacgtccagt
     6421 agggttatgc ggtgagttaa tcaggacaat ttttgttttg tccgtcaaag catcttctac
     6481 tgcttttaca tcaagagaaa agtctgatcc ggaatttact ggttttaaaa ctgctcgatg
     6541 attacccgcg tatgctccat actcggcaaa atatggtttt ggcacaacga tctcgtcacc
     6601 aggattaagc agagcacgaa aaaggacgtt taaaccgcct gctgctccaa ctgtcattac
     6661 aatattctga taattaactt cacatgcatg ctctctggaa gccttttccg ccattgcctt
     6721 tcgcacgtta tcataaccag catttggcat gtacccatga atattctttc gacgatccaa
     6781 agcgatatca ccggctattt tataataagc atcaggcggg ttaagatcag gatttccgat
     6841 tgtaaaatca aaaacatttt cttcaccgag ttttcttttc agctcgatac cttcttcgaa
     6901 catctttcga ataaaggatg acttattaat ggattctttt atatcatctg ctattggcat
     6961 gattttctcc tattgttatt gtttagatta tgttttcttt tccctttacc gaagtaaagt
     7021 tatttttatt taaaaataga tccgtttaag cgctctcaaa ttcttatatt gccaaattca
     7081 tacatactgt ttcaaaacac agtaaaccaa gaagagcagc ctctgacaat caacatataa
     7141 tgttattttg cagaatatag caatttcttg aagctttcct accaagagga tcccgttact
     7201 gtctatttaa agaggtaaca acatgataat tttttatttt atggttatcc gaaagtttag
     7261 tgatgtctct ctttatgatc gaaactgtat tgtcgtcaaa tggataaaaa agaattgtat
     7321 tagacagtat cttgtaatga ccagtgtcct tgcctttgct tgaaagataa aatgcacctc
     7381 cggccgattc aggaataaaa tattcaagat atctatcggc ttgaatatcg ctatttgctg
     7441 aagtataacg caaaaatatg cctgatagct ctccaggata atagagttta ttagatataa
     7501 cagaaaaagc cgattcaact gaagaccttt ttgttaatag atagtacccc tttctaacat
     7561 cggcatcatt tactgaatac tccaagactg ctatatgata tcttcctggt acaagttcaa
     7621 aaaaataaaa tcgatcatct acatgataat cgctctttat aagctcccct gacacatccg
     7681 tttccgaata tatatacagt tcatcgggat catgaaaccg ggcaatattc tttaaaggta
     7741 aggataatcg tatacaaact gcaccggttt ctttaggttt ttccggctca attttttggt
     7801 taatttcaac ttgtcttgcg caagacacta caagtaaaat taagaaaatt tttttcattc
     7861 aacgcacaat cttccttaac aatttttttc ggttttcttc aacggcttgc tgccctattc
     7921 ttagaacctc ttttccacta tcaaaatcta gtccgttgat ctcccttact gcgggctcga
     7981 tcaacacaga tggcttgtta taacgcaaaa gggcaaaggt attcatatac tccatcacct
     8041 ccatcgactg cataaacaca tcaattattg aaggcatttc ataacctttc ggatcttcgt
     8101 ttggctcgtt accattgtcc agtttaagat aagaggacag aaaactgaac catcctgaag
     8161 cattaatctt gatatgtgac gctatacggt tacccatggt tcgttttttt cttctgaaag
     8221 ctatgttttt gagttttttc agcttctttt taccgtgaat taccttagca ctcggatgca
     8281 aattcaccgc tacaactctt ttggcaccca ttttttttac aacatctacc ggtatcgggt
     8341 tggccactcc tccatctata agaatgcggc catcataatc gtatggaaca aaaacccctg
     8401 gtatcgagat actccccctg actgccttaa gaagattgcc ctcccgcatt attaccggat
     8461 tccctgtaaa gtagtcagtt gccactatcg caagtttccc aggaagttct tctatgttaa
     8521 tgtccggatc aataaaccgt tcaagatact tcatgatttt ttttccggca ataattccac
     8581 ttttaggaaa caccggatca accagatgaa caatatctct ccatgaaaaa gatccaaatt
     8641 catttttaaa ctggctgaga gttccagagt agtaaacagc accaactagg gctccaatac
     8701 ttgacccggc aatatagtca attttgacat catgctcatc cagaaattcc agtacattaa
     8761 tatgcgccaa acccttagca ccaccactgg acagtgccac accaattttt cctctgctca
     8821 ttttcgaaaa aaaatcaaac atactattaa cccgtttaat caattctttg aagttaacaa
     8881 aatttaaaat cccatatctt ttaagtcatg aaccatatca acataaaaaa ggtgaaggtc
     8941 tggaacccgt ctgccagaaa actgataaag atctacaata tcgccatgat tgatctgcga
     9001 gacttcacct actttaacga cccccttata aactccccac atggctgact gctcactgac
     9061 aagcccatca tttggaccat ccaggttctt gtgtagttca taaagctttt gccaaaacaa
     9121 cgacggatac tcttcagtaa ttaacgatat ataactttga taatagacct gaggcatatc
     9181 cggaacattc tcattaaagc tctccatata ttccttagta agagaacgcc cggaagagtc
     9241 cggatctggt ctgctgtcac ccattacaac acccacaacc gaagcaagag ttattataaa
     9301 cttaccaccc atttggcgag aatcaatttt ttcgaagaga taatccgcga ccatactgcc
     9361 tcggtgtgga gtgcttattg tagtaagtga cgctacttta tcacccatat ccagacggct
     9421 gatcatgtat cgtgattcta tgccgccctt ggagtgagcg attatattta ctttttgcgc
     9481 cccggtctga gcaataatct gttccactcg atctttaaga aactgagccg ccactttgtg
     9541 ttctgcccag gcattatggt ttgacaaaaa tactgtcgcg cccctgtttc gcagatgatc
     9601 agggatactt ccccaataag gaacaagttt catatcgtca ggcaaggcta taccatgaac
     9661 aaataggatt ggatacttaa gttgacagca tgttgactga agttttacag atcttgaaca
     9721 tgataaaata ttcatgcaca atagaataat taaaacagtt ctataatgcc ggtacatatt
     9781 acaaaccctg gttaaactcg tttaaaagca gctcctcatt gtaatcttta aaaacttcaa
     9841 aaagatttaa tcggtcatac cctgacctga tcaacatctt ctttatatct gttacggtgt
     9901 ctatctcttc tgattgcaga tgttcgatga tcctcttttt tgtgttttga gtcacctttc
     9961 ggtcaatcac tgaatccaca aaataatcta tcaacttttt ctgttttatc gaagaggggt
    10021 aactacgata aaaattgaaa agcatgtcaa aaacctttac ctcttgtact gtgaagtctt
    10081 taatctcttt tgaaaccaga ttagcttctt ttaatgaatt gtaaaaccgg ttaacaattt
    10141 cactatgctt aacagaatag taatcaaccc aaaggctatt ggttttaacc agtaggtttt
    10201 catcaaaagt gcgggaatat tcgtgaaaaa gttctttttt ctctatagca aaaccgttaa
    10261 actcgtgttc ctgccccgga gcaattctaa agagcgaatc gatatccttg attacttgta
    10321 atgttatttc agggtcgtta ttataacaaa acacattctc ttccgatgaa atttcgttac
    10381 gaataatata tttaacaaat tcttcggcaa gaaaagggaa aaaacggccg ttacgtgttt
    10441 ccagatatat aaagtatagc acaaacgagc ggttaaaagg acgtttgcga taatcccttt
    10501 tcttgtccag caaggcgtca taaaaatcgc atagtgataa cgcgtttgta acaacatcaa
    10561 ggtaagattt ttcattcagt ttttcgtcaa gatctctgtc aattatttta gaaaatagct
    10621 gaagatcatc ataattatga tatgactttt ctatcgagtc gtgatggcga aaaacgatat
    10681 ataaaagatc cttgttaaga gagtgaataa agggcttacg ataaagactt tcaaaaaaat
    10741 tatccagatc tttataaaca tcttcgtcgt tatcgataac cttaaaaacc cactgttttg
    10801 tcttctctga cagatttttg tcgttatatc ccgagactgt ttctataggc ccatcacctg
    10861 caaaaaactc gatatcctgt ccgcccaaaa gagcggtaac ggaatgccaa gggtgccttt
    10921 taatctgttg atactcaagt tcagttagtt ttccttcttt atatagtatt ccctgcataa
    10981 caagtagctt gccaatatca tgaacaagtg aagccataag gcaatttttc aaagtccaag
    11041 aagtaagctt tattacctcg gtttccatta gaaaaagcaa aaacttacca taaatttggg
    11101 ttgttcgtat tgcatgttca aatgtgcgct gatgtagatc cctatgagta cacatctgca
    11161 taccgaaaag ggcatcaaag aattgcatac cgatctcaaa attatttttt gatccaagag
    11221 ccttcttaac agcctttata atatcctggg gatcaaaaga taacgacaag accgtttcaa
    11281 cttttaaggt caattgcagc atagtgggga cagccgaatc actgacaagt atcagtgcgg
    11341 catctgtatt gcttttacga aactcattaa gcagaagaaa atggtcttcc cctgcaacct
    11401 gaaatattat tatatcaaat ctatttttta caagaaaatc ggatgttacc ttttctggtg
    11461 aaatataatc aaaatactga aaccctgcct cggcaagaaa aaatgatatg gtttcaaaat
    11521 cattttcgcc tgtacataaa caaaatcttg actcataaaa agagtccagt tcagaatagt
    11581 tgatattgtg cattaaaagt gaccctctgc tatgtacatt aacactgcca gtcatttatt
    11641 taacccgata ttacatgatt accttcaacc caaatccaaa aaacctttac gtaattatca
    11701 attaaacatt acatttttga ttatcggtta aaaaaattct attctttatt gtcaagaata
    11761 tcgcgtatta gccccaccat ctctttgaaa ttcacaggtt taagaagaaa tcctctactg
    11821 ccacacttgc tgatacgatc ggtcatagaa ggatccgagt gagctgtcgt aataatcacc
    11881 ggtatatcat gcttttttaa aataagctct gttgtttcta tcccgtcaag ctcaccctga
    11941 agtgtaacat ccataacaat cagatcaggt ttatcagact gaactcgcga tatagcctct
    12001 tctcctgaaa gaaccggcgg agttacctcg aaaccagcac tctcaagcgt ctgttgtatt
    12061 gccatggtca agataacttc atcttccaca atcattattc ggctcatagt atcatctccg
    12121 cattcacaga cctttgtaat actaacacat taaagttcct catgtgttat gtactcgtaa
    12181 gcagccatcg ccgcggttgc tccgtcggcc gccgctgtaa taatctgttt acgcgatgtt
    12241 gctctgatat cgccagccgc gtaaagtccc ggtattgaag tacccatatt aatatcagtt
    12301 ttaatagctt tccactcgtc ttttatggaa tctggcagca tttccgttac gggattgtaa
    12361 cctacaaaaa taaaaacacc atcaagagtc aaatctctct cggaaccatc cactgtatcc
    12421 tttaaagtag cagaattaac cttattctcg ccatttacag aggtcacaat agaattgaat
    12481 acgatttcta tctttggatg ctccatgacc ctgttgacaa gaatttgact tgcgcgaaac
    12541 ttgtcacggc gatgaataag gtagaccttt gaagcaaacc tggtcaaaaa cagggcttca
    12601 tccaaagcag tattacctcc gcccacaaca gctgttacct tgtcacggaa aaacgcacca
    12661 tcacaggtcg cgcagtaaga aacacctttt ccagtatatt ctttctcgcc tggaaccccc
    12721 aatcgtttat agcgagaacc cgtagcggct ataaccgcac gtgcctcata aacatttccc
    12781 gaagcatctt tgcataccat tttatcttct ttttcatcac gttctacaga ctggatatca
    12841 gcagtaatta tctcggcacc caaacgccgt acctgttttt ccatagcagt tacaagctca
    12901 tatccagtaa tcggatcttc aaaccccgga tagttctcta catcggcagt attaagaacc
    12961 tgcccgcccg gcataaactt ctctatcaca agagttttta ggcccgatcg aacagcataa
    13021 atcgctgcac tcatgccacc cggaccggct cccaaaatta tcagatcatt catttaaacc
    13081 tccgcaatca gcaaaactac aaattaatta ccctgcttgc ccatacatta caacacaaga
    13141 catttttgca acaacaaaca ggcaaaaaac gtttgccgtg acagggcttt tttataccat
    13201 gatacaaata ttataatgct taaacaatga atgttccaca aaatatttaa acaatatatt
    13261 tgaaaatata agcacaagga gagcaataat gggtgaaaaa ataaaactgg aaaagggaat
    13321 tattaccgct cctgacaatc cggttgtatt atttattgaa ggtgatggaa ttggccccga
    13381 tatctggaac gcgacccgaa tcgtaattga tgcggcagtt caaaaggcat ataatgggag
    13441 ccgacaaata gattggaaag agattcttgc cggtgaaaaa tcctttaaca aaactggcga
    13501 atggcttcct gacgagaccg tcaatcttat taaagagtac aaagttgcaa taaagggacc
    13561 tcttacaaca ccagttggag gcggaatacg aagcattaac gtagcccttc gccaggttct
    13621 taaacttttt gcctgtgttc gccccgtacg ttattttagc ggcgtaccct ctgtacttaa
    13681 aaatcctgaa agaacggata tggttatttt cagggaaaat atcgaagatg tgtattcagg
    13741 aatcgaatgg caggaaggaa cagacgatgc caaaaaggtt atcgacttca tcaataacca
    13801 gatgggcaag aatattgctg cagattccgg aatcggaatt aagcctatat ctaaaaccaa
    13861 ttcacaaaac cttattcgca gcgccataca gtatgctata gaaaatgaca aaaaatctgt
    13921 caccctaatg cataagggta atataatgaa atttaccgaa ggagccttta agcagtgggg
    13981 ctacgacctg gttaaagccg agttctctga tactgttatc actgaggatg agctttggga
    14041 taaatatgac ggaaaacagc cagagggaaa agtggtagtc aaagaccgta ttgccgatgc
    14101 tatctttcaa caggttcttt taagacccac tgactatgac gtaatcgcga cacctaatct
    14161 taacggtgac tatatatcag acgcacttgc tgcgcaggtt ggcgggcttg gtatagcgcc
    14221 gggtgcaaat attggtgcca gcgaagccgt atttgaggcc actcacggaa cagcgcccaa
    14281 atacgcaggg caagacaagg ttaaccctgg ctctcttatt ctttccggag agatgatgct
    14341 gcgctttatg aaatggagcg aagcagctga tcttgttgta agcgcaatgg aaaagaccat
    14401 atctcagaag aaggttactt acgaccttga gagacagatg gaaggcgcaa cacttttaaa
    14461 atgttcggaa ttcgcgaacg ccattgtatc taatatgtaa ttttattttc catgcttcct
    14521 cacaaggaag catggaaaaa ttaagccatt attgattgct cattacagcc ggattctgaa
    14581 cagatttatc ttctttcact gttcgactgc cactggtttc acaaagcagc agttccagaa
    14641 gaggcagcat tttttttagt ccgccgcagt tgataatcac cttccaggct tcttcaaact
    14701 ttttccaccc gcctgcgtaa agaagatgct tcattttatg ctttatcgta tcatgagaaa
    14761 acgaagcttt gaggatatta ttccatttat aaaattcttt atacgaccat tgatatccgt
    14821 tttccagttc tcttgcagat aaacccctgg ttttaaatac aacatgcctt gtgtcataca
    14881 tatcccaatt gttggtaatt attcttcccc cttcaaacat tgaattgtaa agccttgttc
    14941 cgggataagg tgtcaatata tgataggtag ctgttgtgac ggcgttttga ataccccagt
    15001 cgactgtccg cttaaaaaca tccgaagcgt cattgtcaaa accatagaca aaactgccat
    15061 taatcataat acccagatcg tgtaatcttt taacggcgta ctcataattc tgcttaagat
    15121 tctggtattt gttactctgc tttaaattct cgacagaaaa ggtttcaaaa cctaaaaaaa
    15181 ggcttcttaa gccggccttt gccgcctttg caatcagatc tccctctatg actgatttta
    15241 ttgttgcagc tccctgaaag acccggttca tgcctctcat tccttcaaaa agctcactaa
    15301 ccagtttagg attacccagc aaatgatcat caagaaaata gaggtgtttc ccgggcaaac
    15361 gtgatatctc tgtcagtatt tcgtcgacac gatgggtata aaaggatatt cctcctttaa
    15421 aaaatgaatc cttataacaa aagtcacaat ggtgagggca tcctctagaa ataacaagtg
    15481 agtttggaac aagatatttt tccctcttaa tcaaatctct tcgaacagga ggcaaatttt
    15541 gtattgtacg accattaccc gaataatacc ttttttttac agtttttgat ttaaaatcat
    15601 ttaaaaattc tgggaatgtc tgctcaccag gtcctatgaa aattgaatcc gcatactctt
    15661 ctgcttcatc cggcaaagct gtaacatgca acccgccaag aatcacaaac acacctttat
    15721 ctctataatg ttttgctatg gaatatgctc tttttgcgtt ggtaatgtat acttggatta
    15781 ttaccagatc gggtgagtca tccagattta atttctcgac atgctgatct tgcaaatcaa
    15841 tttcatcatc tttatcgaga aacgccgcca atgtagccag accaagcgga ggaaataaag
    15901 aatattttat tggacgccaa aaagcgcttc ccgcttctgt caaagacggc aaaatcaatt
    15961 tcactttcat ttaacgcaac ccccgattaa accagataat attgcgaacc ctccgctttc
    16021 c
//
LOCUS       DAOTGZ010000038        16005 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_3064
            NODE_4402_length_16005_cov_4.724075, whole genome shotgun sequence.
ACCESSION   DAOTGZ010000038 DAOTGZ010000000
VERSION     DAOTGZ010000038.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868088
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 16005)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 16005)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:22:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,785
            CDSs (total)                      :: 2,751
            Genes (coding)                    :: 2,732
            CDSs (with protein)               :: 2,732
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 12 of 19
            Pseudo Genes (internal stop)      :: 5 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16005
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4402_length_16005_cov_4.724075"
                     /isolate="AMR_MDS_3064"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..432)
                     /locus_tag="P5123_04180"
     CDS             complement(<1..432)
                     /locus_tag="P5123_04180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015682574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecA"
                     /protein_id="HRX15490.1"
                     /translation="MFGKIVSLIFGTKHERDVKRMKPIVAAVNSFESVMQKKSNDELR
                     DVSFDLRERCIKGESLDDLLPEAFALVRETAYRVLGMRHYDVQLMGGIVLHQGKNAEM
                     KTGEGKTLVATLAVYLNALSGKGVYVVTVNDYLARRDANWMR"
     gene            complement(543..1652)
                     /gene="proB"
                     /locus_tag="P5123_04185"
     CDS             complement(543..1652)
                     /gene="proB"
                     /locus_tag="P5123_04185"
                     /EC_number="2.7.2.11"
                     /inference="COORDINATES: protein motif:HMM:TIGR01027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004349 - glutamate 5-kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006561 - proline biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate 5-kinase"
                     /protein_id="HRX15491.1"
                     /translation="MPRKDYLKNVRRVVVKIGSSSITENGCISENRIDTFVRDIAAVK
                     SKGYEVIVVSSGAISAGAGEAKIALEHLAIPVKQALAAVGQTVLMNSYRHAMQQHGIM
                     AGQILMTEDDVKNRRRFLNLRNTINALLKMNAVPVINENDSVAVQEIKVGDNDTLSVH
                     VAHMAEAQLLILLSDVDGFYRDLSDDEPIDVVHKIDENIRAMAGGSGSRYGTGGMQTK
                     INAADKVIKSGEMMVIASAAEKSVIERIVSGEKLGTLFIHPDDVTMPGRKRWISFNMN
                     EKGSVTVDDGAYRALAEKKKSLLAIGVVSVEGSFSPGDAVSIIHNNRAIGKGVVNYSN
                     DELNKIAGVRSDKIASVLGREYYDEVIHRDNLIVF"
     gene            complement(1707..2693)
                     /gene="obgE"
                     /locus_tag="P5123_04190"
     CDS             complement(1707..2693)
                     /gene="obgE"
                     /locus_tag="P5123_04190"
                     /inference="COORDINATES: protein motif:HMM:NF008955.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase ObgE"
                     /protein_id="HRX15492.1"
                     /translation="MPKFTDSIRIKIKAGQGGPGSVSFHREKYIAKGGPDGGDGGKGG
                     NVYFVADRGRTNLSSYFKDRLYAAKRGMHGQGRNKHGSDGEDLLLKVPPGTEIIREDT
                     GELLSDMIEEDKPVLIASGGQGGHGNAFFKSATMQIPRFAQPGEEGEEFTVILNLKLI
                     ADVGIVGLPNAGKSTFLKAVTNAKPKIGSYPFTTLIPNIGVVKSPRGKEYKIADIPGI
                     IEGAHQGSGLGLSFLRHIERVNCLLYFIDIESEDIAYTYSLLRNELSSYSEALLNKPS
                     RIVLTKADLVPDKGFIDDIIKDLKMDNIMAVSSVDGYNTDKVLKEVESMLKY"
     gene            complement(2999..3253)
                     /gene="rpmA"
                     /locus_tag="P5123_04195"
     CDS             complement(2999..3253)
                     /gene="rpmA"
                     /locus_tag="P5123_04195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010476817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L27"
                     /protein_id="HRX15493.1"
                     /translation="MAHKKGGGSTRNGRDSNAQRLGVKKFDGELITGGTILVRQRGTK
                     IHPGTNVGKGSDDTLFSKITGKVKFERYGKTRKKVSVYPA"
     gene            complement(3263..3580)
                     /locus_tag="P5123_04200"
     CDS             complement(3263..3580)
                     /locus_tag="P5123_04200"
                     /inference="COORDINATES: protein motif:HMM:NF016233.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosomal-processing cysteine protease Prp"
                     /protein_id="HRX15494.1"
                     /translation="MVTVSFYSLTSDSKRILTVEGHAGFRKKGSDIVCSAVSVLVHTF
                     ISASRLVAGVSLTVYDGGVLEVNVDKIDNVDAFSVILETVAVGLEDVSREYPQNVQVH
                     YIK"
     gene            complement(3581..3892)
                     /gene="rplU"
                     /locus_tag="P5123_04205"
     CDS             complement(3581..3892)
                     /gene="rplU"
                     /locus_tag="P5123_04205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014297125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L21"
                     /protein_id="HRX15495.1"
                     /translation="MYAIVEIGGKQYKVEKGMSIDIEKIKAEPETSITLDKVLMVVDG
                     DKVNLGKPYVSGASVKASVVSEVKGKKVLGVKFKKRKNYTRTVGHRPLYLTLKIDELT
                     A"
     gene            complement(3919..3992)
                     /locus_tag="P5123_04210"
     tRNA            complement(3919..3992)
                     /locus_tag="P5123_04210"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(3956..3958),aa:Ile,seq:cat)
     gene            complement(4230..4697)
                     /locus_tag="P5123_04215"
     CDS             complement(4230..4697)
                     /locus_tag="P5123_04215"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRX15496.1"
                     /translation="MKKILLFLLILVFASLSFVSCDDESESTKTLEEKMEGVWEWIDT
                     ETETEIFVPAVEYDLYGKYTQFSKGKRREYSFDSLKNNLYYCIDDDATYTVDEKTKIY
                     SFINADGEIKFANVSFDDNYMILDYSESNGNKWITKMKKVDPSVIDAAYEYCD"
     gene            5176..7185
                     /locus_tag="P5123_04220"
     CDS             5176..7185
                     /locus_tag="P5123_04220"
                     /EC_number="4.6.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF017076.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009975 - cyclase activity [Evidence IEA];
                     GO_function: GO:0016849 - phosphorus-oxygen lyase activity
                     [Evidence IEA];
                     GO_function: GO:0032555 - purine ribonucleotide binding
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate/guanylate cyclase domain-containing
                     protein"
                     /protein_id="HRX15497.1"
                     /translation="MIKFKKTHILSLIASLAVVLFIHLLSTFNLLNGLENMSLDFRFF
                     MRDTTQKPVDLKEGVVVNQNNPLASEEIMIAGIDIETIRYFDEQGIQWPFPWDIHAKT
                     TEFIATGNPNTILYDIMFLEQKPGEDKLATAFKNAGCVIPDYALEKDTTHRKPSNQNE
                     RIAILDNFVLPIAPEKAPSIFKEIDAPIPSIASASRSIGFANVLEDSDNTIRSTLLYA
                     RVADKVYPSVVLCTIMNYYNVTVDDLEIVPGSYIRINNPDPTKSGKHYPDGIIEIPID
                     HNGLMPINFIGTHGSFNKVPYFYFHNEGSMEGALGNKIVMIAVYAASGIADDMKNSPY
                     GQMYGIEQHAHAINTILTNSYLYKFSYRQTLAIIIFIALIFGLLIPRLSIIKSTIATI
                     ILAIGYILTAHVLFEKQNLIIAYATPVLFIIIDHIAITSVRMFTEQKEKRMIRQTFSK
                     FVSKAVVDELLRVPGKVKLGGERKILTVLFSDIRGFTTLSEKLTPEELVAHLNIYLQA
                     MTDIVIKYNGTLDKYVGDEIMAFWGAPIPQQHHAYLACRAALDMMTELNKLNEKWASE
                     GNSHLCLNIGIGINSGEMVAGNMGSAARMDYTLMGDNVNLGARLEGTNKMYGTAVIIS
                     EFTYEMIKDEKIVVRELDLIRVKGKAKPVRIYELVDIEERPDKGV"
     gene            complement(7197..9467)
                     /locus_tag="P5123_04225"
     CDS             complement(7197..9467)
                     /locus_tag="P5123_04225"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRX15498.1"
                     /translation="MKNKLRILTVLIVLCAICALTAVLFLSYNLKKRYDSGLLRNLVV
                     GFVENKFDKAVRFDSIGMSYSGDIVIKNLKISAFNDFNDNILLVDSPKTVIELDLFSL
                     FNGEIQIEDVSIYSANFTIHKAYDALYGEAFSVVTGIYKQFAQSKELSKKKISLKIRD
                     SSFTYSEIFRSDALRFTASSFNLDIAITRDEIKYSAKGAFNKGGTPFIDESPFTVGGT
                     IFFNDGKFHGTSVEYSVVNLDLTYLNAYLKFKSGLSHEIAGAMSSKGAVNILRDNATI
                     NSESSFTGLSLKNVSRVEDSFTLVNNKNLKASLNLDILNGGEVVVARRFLLDDDNLYI
                     MAEGEYVNTELSHYLKLNARSNRIDLNQLSGTFRPVKNVSYSGGLQFDFSTDYNINSG
                     KNNRSSVVLHLNDFSAERIEDGLAKSFFRNTDVKISGTEKQLDAKADARSFGSDYKIT
                     IKSELDSWVPVAGNTVIDISSEQLEASLITRLVAEGQLYLENTGLADTKEGYDGVMFP
                     DKEYGKFLTNNSIDLSFRCGSLVFRNNAQIKNIVLRQILEDKMLSTETFSASGYDAQY
                     ILDLYGNFKGMYAKVQAKAGVYDFNFARFFKDTGARGEVGGTLRGEYSWSVDGYRLRD
                     LVLNNRSSFELSLKNGYVKGTKFLDEYYTFLASEGVTAQPETVEFSDATYRLKKNSLS
                     CYTSALLTGKSASISSSGPYLFGEGYSMPLRISLTEDNGDKIRRESGQFLLTGVFTSP
                     VLQSRTDPDMSIELFR"
     gene            9707..10342
                     /locus_tag="P5123_04230"
     CDS             9707..10342
                     /locus_tag="P5123_04230"
                     /inference="COORDINATES: protein motif:HMM:NF020893.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DedA family protein"
                     /protein_id="HRX15499.1"
                     /translation="MINSIADAFFTFFSHHSHFLPWISFGLLLLAGLNLPISEDLVLI
                     LSGAITALYAPELEHYIFIGCFLGAYLSDIMAYFIGRCFIHRVVNSNFLSHIFHPERI
                     ARINGYFRKYGEKVIFFGRFIPFGVRNLVFMTSGLTRVPLKHFLIIDFLALIITSTIL
                     FQLGIKFGEDYTSLLKILNQYKTIFALVAVVAVIIIVKYEIKVHRNKKIQD"
     gene            complement(10339..10698)
                     /locus_tag="P5123_04235"
     CDS             complement(10339..10698)
                     /locus_tag="P5123_04235"
                     /inference="COORDINATES: protein motif:HMM:NF024773.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CoA-binding protein"
                     /protein_id="HRX15500.1"
                     /translation="MKNVVLIGASSKEDSYSYMAFKLLKEKGFNVIPVHPFNKEIDGI
                     KVRRDLDGLSDIYAVTLYVNPVILKNYEEQIVAIKPQRVIFNPGTVSPDADKRFSKAS
                     ITTVEACSIVLLKTDRF"
     gene            10929..12713
                     /locus_tag="P5123_04240"
     CDS             10929..12713
                     /locus_tag="P5123_04240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013629899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HRX15501.1"
                     /translation="MALITLQNITHTFGALPLLDSITVHIEQGEKIALLGRNGSGKST
                     LMKIFSGEILPDSGTVITDKYFKCALMPQDVPSSIDGTISSVISSGLADPSDLSRVEY
                     IISLLDLDKNSQFETLSAGMKRRVLLGYALVSDPDLLLLDEPTNHLDIASITWLENFL
                     VKYRGTILLVTHDRSFMQNITNRIIEIDRGRLFDWKCGYNIFLDRKNEWLENEDTQNR
                     VFDKKLAEEEVWIRKGIKARRTRNEGRVRALMKMRDEHKARRSAQGNAKIEVNQASQS
                     GKMVVEAENITFGYDKTLIDDFSIRIVRGDKIGIIGANGCGKSTLIRIILGLLPVQSG
                     SVRTGANLEIAYFDQLRNQCDDNLTVIDNVSNGCETVTINGRDRHIISYLQDFLFTPE
                     RAHEKSGVLSGGEKNRLLLAKLFTKPANVLVLDEPTNDLDIETVELLEELILDFNGTV
                     LMVSHDRSFLNNTATDIISFEPDGKLVCCAGGYDDYLSQRTPLNTIDVKKTESETTKA
                     KPKSEKIKLSYKENIEIEKLPHDIEKLENKIEDLHAKLGDPELYKTNGDSVNQIKDEL
                     ESLEVELETKFSRWEELEEKRLRAETES"
     gene            complement(12737..13807)
                     /locus_tag="P5123_04245"
     CDS             complement(12737..13807)
                     /locus_tag="P5123_04245"
                     /inference="COORDINATES: protein motif:HMM:NF012376.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HRX15502.1"
                     /translation="MTKYSILFVILALLSCDVPQNENYELDKKVGKASVDGAQVEVVS
                     GLGVIKNIEPGKLEIHQSAPQIELVIDPGKSGISAWQIVLSNSVRNTYALSDDSVTIL
                     SENQNGIKYSLECEVPVNKKISIYFKNTEEDPVFAVFSDVQRAVSEVSDIFEVINSDD
                     DLQFVLSTGDLTSNGTKSEIKRFIKELEYLAVPLYTTMGNHDSFDSQYKYWHQVFGRT
                     SFRFVYRDTLFVFLDSSSGALYHELYDDLEGWLGLSDGLKIVLTHYPIVDPSGTRGGS
                     FSSRNEAYKLLSLLGENGTDLLLFGHIHSYYKYTLAGIDCRISGGGGAMPEKYDGIGR
                     HYLKIEVDPDNEKYTIVRVDID"
     gene            complement(13866..14936)
                     /locus_tag="P5123_04250"
     CDS             complement(13866..14936)
                     /locus_tag="P5123_04250"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRX15503.1"
                     /translation="MLFKRLVIYLLLLLLLNLDTALSAEEQSSYKEVILPPVAAVVPG
                     SVLHGAGHFVAGERETAGELLLIEGVGLLMLAGSGYILADGGAPRDYTGPLVPFAAFG
                     VSFLVLPFIADFYGVTIGRYRSEGDWFTDKNKTVTLNAGLEYTNDPAFDYSLLAKAGF
                     DYHYKRFVISPMINSAIDDDTRHYQLSAKYRFAGQKGHLLENYATFFKSDAIVRAGYY
                     DFNSDGFYKYSTDISFRTVINSGSLWKTTRGLYFVYEVGFNREWVYFDFDETLPAITS
                     NQILFETGVGFLTGDLSDSKADIYLFYNHRRDTFYGGLPSGFLGHVGASVTVYPWQNT
                     GISLKAVLGTASFYSLSGKLSF"
     gene            15300..>16005
                     /locus_tag="P5123_04255"
     CDS             15300..>16005
                     /locus_tag="P5123_04255"
                     /inference="COORDINATES: protein motif:HMM:NF012551.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endo-1,4-beta-xylanase"
                     /protein_id="HRX15504.1"
                     /translation="MNYSFLKTTLLRKPVLFVLLTSVFISCSRDPLKEYDSQIETDLM
                     PIKEFYKDDFPIGAAIEPFQVEKAEGALLRYHYNSITCENAMKFKSIHPKQKVYNFKA
                     ADKIAEFAKRYNMKMRGHTFVWHHPAEQVFWIFLNNDGSKRSRESVLELLGEHMQTLV
                     DRYGDTIYAWDVVNEAIDIAEPDNMRKSQWYHSIGPDYVTEAFKLARKAAPDALLFYN
                     DYDFFNPIKRDAIYDLV"
BASE COUNT         5011 a         3467 c         2818 g         4709 t
ORIGIN      
        1 gcgcatccag tttgcatcac ggcgcgcgag gtaatcgttt acagttacta cataaactcc
       61 ctttccgctt aatgcgttaa gataaacggc caatgtcgcc acaagggttt taccctctcc
      121 ggttttcatc tccgcgtttt taccctgatg taaaacgata ccacccataa gctgaacatc
      181 ataatgacgc ataccgagaa cccgataggc tgtttcacgc acaagagcaa aagcctctgg
      241 caaaagatca tcaaggcttt caccttttat acatcgttca cgcaaatcga atgagacgtc
      301 ccgaagttcg tcatttgatt ttttctgcat taccgattca aaactgttta cagcagcaac
      361 aatcggcttc attcgcttta catcacgctc atgttttgtt ccaaaaatca gactgactat
      421 tttaccaaac atctattatt cctttttatt ataatctttt tatttaatta ctaacgacct
      481 tgcatgcttt gcaaaaatac gtcaagttaa agctgtttat tgaggtttgt tgcatcaaaa
      541 aattaaaaaa ctatcaaatt gtccctgtgg ataacctcat cgtaatactc gcgccccaaa
      601 acggaagcaa ttttgtccga acgaacacct gctattttat taagttcatc attgctatag
      661 ttaaccacac cttttccgat agctcgatta ttatgaatga ttgataccgc atcacctgga
      721 ctaaatgatc cttcaactga aacaacacca atagccaaca gagacttctt tttttcggca
      781 agagccctgt atgctccgtc gtctacagta acagaacctt tttcattcat attaaaagat
      841 atccaccgct ttcgtccagg catggtgaca tcatcgggat gaataaaaag tgttcccagt
      901 ttttcaccag acacaattct ttcgattacc gatttctcgg cagcagaagc gataaccatc
      961 atctctccag actttataac cttatcggcc gcgtttatct tggtttgcat cccacctgtt
     1021 ccgtaacgcg atccagagcc gccagccatt gcccggatat tttcatcaat cttatgtaca
     1081 acatctattg gttcgtcatc agataaatct ctgtaaaatc catccacatc tgataacagt
     1141 atcaaaagtt gcgcctcggc catgtgagct acatgaacag acagcgtatc attatctccg
     1201 acctttattt cctgaacagc aacggaatca ttttcgttaa tgacgggtac agcgttcatc
     1261 ttaagcaacg cgtttatagt atttcgcaag ttcaaaaacc tgcgacggtt ttttacatca
     1321 tcctcggtca tcaaaatctg gccagccatg ataccgtgtt gctgcatcgc gtgacggtac
     1381 gaattcataa gcacagtctg cccaaccgca gctaatgcct gtttaacagg tattgcaaga
     1441 tgctcaagag caatttttgc ttcaccggca ccggcagaga tggctcccga agatacaaca
     1501 atcacctcat aacccttgga tttaaccgcg gctatgtctc gtacaaaagt atcgattctg
     1561 ttttcactta tgcagccatt ttcggtaatc gaagacgagc ctatctttac aaccacgcgg
     1621 cgaacatttt ttagataatc ttttcttggc atactgtttt cccagatata ttagtgagtt
     1681 ctttaaaata ttctattgtt acaattttag tactttagca tggattcaac ttcttttagc
     1741 accttatcgg tattgtagcc atcaacagaa gagacagcca taatattatc cattttcaga
     1801 tcctttatga tatcgtcaat gaatccctta tccggaacca gatcagcttt ggttaacaca
     1861 atacgactag gcttgtttaa aagagcctcg ctgtacgatg aaagctcatt acgtaaaagc
     1921 gaataggtat aagctatatc ctcggattcg atatcgataa aatagagaag acagtttaca
     1981 cgttcgatat gtcgcaaaaa ggagagcccc aatccgctac cttgatgcgc gccctctata
     2041 atacccggaa tgtccgcgat tttgtactct tttccccgag ggcttttaac aacaccgata
     2101 ttaggtatca gtgtagtaaa ggggtagctg cctattttgg gttttgcatt ggttacagct
     2161 ttcagaaaag tggattttcc agcgttggga agcccaacaa tacctacatc cgctatcagt
     2221 ttaagattta aaataacagt aaactcttct ccctcttccc caggctgggc aaatcgcggg
     2281 atctgcatgg ttgctgattt aaaaaaagca ttaccatgcc cgccctgtcc gccacttgca
     2341 atcaatacag gtttatcttc ctctatcatg tcagataaaa gttctcccgt atcttcacgt
     2401 ataatttcgg tgccaggcgg aactttaagc agaagatctt ccccgtcaga accgtgtttg
     2461 tttcggccct gcccgtgcat acctcttttg gccgcataaa ggcggtcctt aaaatatgac
     2521 gacagatttg tacggcctcg gtcggcgaca aaatagacat taccaccctt tcctccgtcg
     2581 cccccgtcag ggccaccctt ggcgatgtat ttctcacgat gaaaagagac cgatcccggt
     2641 cctccctgtc ccgcttttat ttttattcta atggaatcag taaattttgg cataacaaac
     2701 ctgtactggt gttaaaaaaa ccggcactac tccgtaaaag atataatccg gactttaaac
     2761 accattttaa tttttttatg taatgcagga acttcatttt gtaccattat cattagacct
     2821 gattgtctta caatcattaa aagaatattg aaaaccttaa ataattatta tcaaggatat
     2881 tgtttcaatt tgccgagggg taaaactcga ggcagactac aaaaccatgc acgaaaaaga
     2941 gcgcaagaaa actcacgctc ttttaaccag acagcctata ttaagacgta ttaaaatatt
     3001 acgcaggata gacagaaact ttctttcttg tcttgccgta acgttcgaat ttaacttttc
     3061 cggtaatctt tgaaaaaaga gtatcatccg atccttttcc gacattggtt ccgggatgaa
     3121 tcttggttcc acgctgacgt acaagaattg taccgccagt aataagttca ccatcaaact
     3181 tcttcacacc aagacgctgc gcgttggaat cccgaccgtt tctggtagat ccgccacctt
     3241 tcttatgtgc catatcaatt cctcatttta tgtaatgtac ctgtacgttt tgcgggtatt
     3301 cacgggaaac atcttctaac cccacagcta ccgtctccag aatcaccgaa aacgcatcaa
     3361 cattatctat tttatcaaca tttacctcaa gcactccacc gtcataaacc gtaagtgaaa
     3421 caccagcgac aagccgtgaa gccgagataa aagtatgcac taaaacggaa acagccgaac
     3481 agacaatatc acttcccttt ttacggaaac cggcatgccc ttcgaccgtt aaaattctct
     3541 tactatctga cgtaagagaa taaaatgata ccgtaaccaa ttacgcagtc agttcgtcaa
     3601 tcttgagagt aagataaagc ggacgatggc caactgtacg agtatagttt ttacgttttt
     3661 taaacttaac gccaaggact tttttgccct ttacttcgga taccacagaa gcctttacgg
     3721 aagctccgga cacataaggt ttaccaaggt taaccttatc accgtctaca accataagaa
     3781 ccttgtctaa agtgatagaa gtctcaggct cggccttgat cttctctata tcaattgaca
     3841 tacctttttc aactttatac tgctttccgc ctatttcaac aatcgcgtac attgttatta
     3901 ctccaaattt aatgaatgtg ggccctgtag gactcgaacc tacggcctac tgattatgag
     3961 tcagccgctc taaccaactg agctaagggc ccctgatcgc aagtgggaac actaaaacaa
     4021 caggcatgtt ttttgtcaat aaaatattgg gagtggcgaa tgaaaaaaca ttatgtctct
     4081 aatcgcttat tcaaatccaa gttttcaaaa gaagtatata ctaacataca ggactcttta
     4141 agagagccct gtaatattta atcaaattaa tgtgaaaatt tcgtgctatc agaccaagcc
     4201 ttacactatc aagcaaaaat ggtagccaat taatcgcaat attcgtaagc tgcgtcaatt
     4261 accgatggat ctactttttt catcttagtt atccatttat taccattact ttccgaataa
     4321 tccaaaatca tatagttatc atcaaaagaa acattagcga atttaatttc gccatcggcg
     4381 tttataaaag agtaaatctt tgttttctca tccactgtgt atgtagcatc atcatcaata
     4441 caatagtaca agttgttttt taatgagtca aaagaatatt ctcgacgctt ccctttacta
     4501 aactgagtat attttccata aaggtcatac tcaacagccg gaacaaaaat ttcagtctca
     4561 gtctcagtat caatccactc ccagacacct tccatttttt cttcaagagt tttggtgctt
     4621 tctgattcat catcacaaga gacaaacgaa agtgaggcaa aaacaagaat caaaagaaac
     4681 aacagtattt ttttcataaa acctaccttc tataattatt ttttatcatt atatatatat
     4741 atatatatat atatatcaag caatttttaa tcataagcat tttgatatta agcttaataa
     4801 atcatcaata ctgacattaa ctcacatgtt tttattccat aaacaaacac aattctgtaa
     4861 tttaagcatg atttcatctt ggaccttaat cataaatgat aaatattatc actaacacgt
     4921 tatttataga acttttcttg aaaatgtaac gtttatagat tttaaaccat ttattaattt
     4981 taaaaaagca tcgtaaaatt attgcacaag aaggcgatga atatgataat catcaaagat
     5041 tataactgtt cttttaccta aaaaaacatt gatatatttg aaacctgttt tattatcagt
     5101 aatatacatg aaaattcaga tttctaattt aatcattttc gcatctatca aaaaaccgtc
     5161 aaggaacatg ataccatgat aaaattcaaa aaaacccata ttctgtcact aatcgcgtca
     5221 ctagcggttg ttctatttat tcatcttctc tcaactttta acctgcttaa cgggcttgaa
     5281 aacatgtcgc ttgactttcg cttttttatg cgagacacaa cccagaaacc ggtcgacctt
     5341 aaagagggag ttgtggttaa tcaaaacaac cccctcgcct ctgaagagat catgatagcg
     5401 ggaatcgata ttgaaacaat cagatatttt gacgaacagg gaattcaatg gccttttcca
     5461 tgggatattc acgcaaaaac taccgaattt atcgctacgg gtaatcccaa tactattctt
     5521 tacgatataa tgttcttgga acagaagccg ggtgaagata aacttgccac cgcgtttaaa
     5581 aacgctggct gtgttattcc cgactacgcc cttgaaaaag atacaactca tcgaaaacct
     5641 tctaaccaaa acgagagaat cgccatactt gacaattttg ttttacccat agcccccgaa
     5701 aaagcaccct caatatttaa ggagatagac gcgcctatcc cttcaatagc cagcgcctcc
     5761 agaagtatag gctttgcgaa tgttcttgaa gattctgata acacgataag aagcaccctt
     5821 ctttatgcac gcgttgcaga caaggtttac ccgtcggttg ttctctgtac aatcatgaac
     5881 tattacaacg taacggttga cgatctggaa attgtaccgg gatcttatat acggataaat
     5941 aatcccgacc ccacaaagtc aggaaaacat tatcctgatg gtataatcga gatccccatt
     6001 gatcacaacg gacttatgcc aataaacttt ataggaacac acggatcatt taacaaggta
     6061 ccatatttct attttcacaa cgaaggctcg atggaaggtg ctcttggaaa caagatagta
     6121 atgatcgcgg tttacgcggc ctccggaata gccgacgata tgaaaaattc accgtacgga
     6181 cagatgtatg gcattgagca gcacgcgcac gcaataaaca caatactaac aaacagctac
     6241 ctgtacaaat tctcttacag acaaacactt gctataatta tttttatcgc acttatcttc
     6301 ggtttgctta ttccgcgcct ctcaattatt aaatcgacca tagcaacaat aattctggct
     6361 attggctata ttttaacagc tcatgttctt ttcgaaaaac aaaatttgat tatcgcttac
     6421 gcgacacctg tattatttat aattattgat catattgcca tcacatcggt aaggatgttt
     6481 acagaacaga aagagaagag aatgatacgt cagaccttct caaagtttgt atccaaagct
     6541 gttgtagacg aacttttgcg ggttcccggc aaggtaaaat tggggggaga gcgcaagatc
     6601 ttgacagttc ttttttccga cataaggggc tttacaactt tatctgaaaa actgactccg
     6661 gaggagctgg ttgcccacct aaatatttac ctgcaggcta tgacggatat tgttatcaag
     6721 tacaacggca cactggataa atacgttggt gacgagatca tggctttctg gggtgctccg
     6781 attcctcaac aacatcacgc ctatctggca tgcagagccg ccctcgatat gatgacagag
     6841 ttgaacaagc ttaatgagaa gtgggcgtcc gaaggcaatt ctcatctttg tctcaatatc
     6901 ggtatcggta tcaactccgg agagatggta gccggcaata tgggttcggc cgcgcgtatg
     6961 gactacacac ttatgggaga taatgtaaac cttggagcaa ggctagaagg aacaaacaag
     7021 atgtatggaa ccgctgttat tattagcgaa ttcacatacg aaatgattaa ggatgaaaag
     7081 atagtagtac gcgaacttga ccttattcgt gtaaaaggaa aagcaaagcc agtgagaata
     7141 tacgaacttg ttgacattga agagagacca gataagggcg tttagtcatc gccaaattat
     7201 ctgaacagct caatcgacat atctggatca gttcgcgatt gaagaacagg gcttgtgaat
     7261 actcctgtca gaagaaactg tccactttca cgacggattt tatctccatt gtcttctgtt
     7321 aagcttatac gaagcggcat gctgtatccc tcaccaaaca gatagggacc gctactgctt
     7381 attgaagcag attttcccgt taataaagca ctggtgtaac atgagaggct attctttttt
     7441 aaacgataag ttgcgtccga gaattctaca gtctcgggtt gtgccgtaac accctccgat
     7501 gcaagaaaag tataatattc gtcaagaaac ttggttccct taacgtatcc gttttttaat
     7561 gataattcaa atgaagatct gttattcaac acaagatcac gcaagcggta tccgtccaca
     7621 ctccatgagt actcccctct caaggttcca cccacttcac ctcttgctcc agtatcctta
     7681 aaaaatcttg caaaattaaa atcataaact ccggccttgg cctggacttt agcgtacatt
     7741 cccttaaagt ttccgtaaag atcaagtata tactgtgcgt catatcccga ggcactgaag
     7801 gtctcagttg acagcatttt atcttcaagg atctgcctta gaacgatatt cttgatttgc
     7861 gcattattac gaaaaacaag tgaaccgcat ctgaacgaaa gatcaatgga gttatttgtc
     7921 aaaaacttgc catactcctt gtcgggaaac atgacgccgt catatccctc ttttgtgtcg
     7981 gcaagacctg tattctccag ataaagttgc ccctctgcga ctaaccgggt aatcaaggat
     8041 gcttcgagtt gctctgatga aatatcaatg acagtgtttc cagctactgg cacccagcta
     8101 tcaagttctg attttatggt tattttataa tcagaaccga atgatcgtgc atcggctttg
     8161 gcatcaagct gcttttctgt tccagatatt ttcacatctg tgtttctgaa aaatgattta
     8221 gccaggccgt cttcaatacg ctcggcagaa aaatcattaa gatgcagaac aacgctgctt
     8281 ctgttatttt taccgctgtt aatattataa tctgttgaaa aatcaaactg caacccgccg
     8341 gagtaagaaa catttttaac cggtctaaat gttccggata attgattaag atctattcta
     8401 tttgaacgcg cgttaagttt tagataatgt gataattcgg tatttacata ttcgccctcg
     8461 gccattatat aaagattatc atcatccaaa agaaaacgac gtgctacaac gacctcccct
     8521 ccgtttaaga tatcaagatt cagggacgcc tttaaatttt tattatttac cagagtaaaa
     8581 gagtcctcga ctcgggaaac attttttagt gatagccctg taaatgatga ttcagaattg
     8641 attgttgcat tatcacgtaa aatatttacc gcaccctttg aactcatggc tccggcaatt
     8701 tcgtgactta atccagattt gaatttaagg taagcgttaa ggtatgtcag atccagattc
     8761 acaacggaat attccacact tgttccatga aacttaccgt cattgaaaaa tattgttcca
     8821 ccgacagtaa aaggtgactc atcaataaac ggagttccgc ccttgttaaa agcacctttg
     8881 gctgagtact ttatttcgtc tctggtaatg gcaatatcaa ggttaaatga ggaggctgta
     8941 aaacgtaaag catcacttct gaaaatttca ctgtaagtaa aggacgaatc acgaatcttt
     9001 agacttatct tttttttcga aagctctttt gactgagcaa actgcttata aatccccgtt
     9061 actacggaaa aagcttcacc gtaaagggca tcgtaagcct tatgtatagt aaaattggcc
     9121 gaatatatac tcacatcttc aatttgaatt tctccgttaa aaagtgaaaa gagatcaagt
     9181 tcgataaccg tttttggaga atcaacaaga agtatattgt cattgaaatc attaaaagcc
     9241 gatatcttta aattctttat aactatatcg ccgctataac tcattcctat tgagtcaaaa
     9301 cgaactgctt tatcaaattt gttctctaca aatccgacaa ctaggtttct tagcaatcca
     9361 ctgtcgtacc ttttttttag attatatgat aaaaacagaa ctgcagtcaa ggcacatatc
     9421 gcgcacaaaa caatcaagac ggttaatata cgtaatttat ttttcaatgg tattaaatcc
     9481 tttcgatagt taattagaag cagttttact gcaaagatac ttatggtctc agggactaac
     9541 tgctaaaagg gtttgaacta tttgtcataa agtcaaggaa aataagagtt aaagtagaga
     9601 taacccttgt tattcaattt gcttatgaaa aaattatgtt gccaaatttt aatgataagt
     9661 attcatgggg tgaataattt taccactagg ctcggaagat aatcgaatga taaacagtat
     9721 agcagacgct tttttcacct ttttttcaca tcactcacat tttttaccct ggatatcttt
     9781 tggtctgcta cttcttgccg gtttgaatct tccaatatcg gaagatcttg tattaatcct
     9841 gtccggtgct attacagctc tttacgcgcc cgaacttgaa cactatattt ttattggatg
     9901 ttttttggga gcatacctga gcgatattat ggcctatttt atcggtcgtt gttttataca
     9961 ccgtgttgtt aattcgaatt ttctctcaca tatttttcat cccgaacgaa tcgccagaat
    10021 aaacggttat tttcgtaaat atggagaaaa agtcatcttc tttgggcgat ttataccttt
    10081 cggagtacga aatcttgttt ttatgacatc tgggctcacc cgtgttcctc taaagcattt
    10141 cctgataatt gattttttag ctcttatcat tacctctaca attctttttc aactaggcat
    10201 caaatttggt gaagactata caagccttct taagatttta aaccagtaca aaacaatttt
    10261 cgcgcttgta gccgtggtcg cggtaatcat tatcgttaaa tacgagatta aagttcaccg
    10321 taacaagaaa attcaagact aaaatcggtc agtttttaat aacactatcg aacaggcctc
    10381 gacagtagta atggacgcct tggaaaaacg tttatccgcg tcaggggaca ctgttcctgg
    10441 gttaaaaata actctttgcg gttttatcgc aactatctgc tcttcatagt tttttaatat
    10501 tacgggatta acatatagtg taactgcgta aatatctgat aaaccatcca gatcacgcct
    10561 taccttaata ccgtcgatct ccttattgaa gggatgcacc ggtatgacat taaaaccttt
    10621 ctctttaagc agcttaaagg ccatatacga ataactatct tctttgctgc ttgctcctat
    10681 taaaacgaca tttttcataa ccgtccccct ggtatattta tactttacct ttaaaaccat
    10741 catccgacgg tcttggtcta tattgcttgt taaaatgaaa atgtcaaaga agaaaatact
    10801 aatagtatta caagggtggt tttgcgtttt gacaaccaat aacacgaaaa attctttaca
    10861 acaacagccc tgaaagagtc gtattaaccg aaacaaaaaa gtttaacaaa aaccagagac
    10921 ggaacaaaat ggcacttata acacttcaga atataaccca cactttcgga gcattacctc
    10981 ttctagactc cataaccgtg catatcgaac agggagagaa aatagctctt cttggacgca
    11041 acggttcggg taagtcgaca ctcatgaaga tatttagcgg agaaatttta ccagatagcg
    11101 gaacagttat aacagacaaa tatttcaaat gcgccctgat gcctcaggat gtgccatcat
    11161 caatcgatgg cacaatttct tctgtaattt cttcgggcct tgcggatcct tccgacctga
    11221 gtcgtgttga gtatattatt tctcttcttg atctggataa gaattcacaa tttgaaactc
    11281 tttcagcagg catgaaacgt cgtgtacttc tcggatacgc gcttgtttca gaccccgacc
    11341 ttttgcttct ggacgagccc acaaaccacc ttgatatcgc gtcgatcacc tggcttgaaa
    11401 attttttagt aaaatacagg ggcacaattc ttttagtaac acacgaccgt tcctttatgc
    11461 aaaatattac gaaccgtatt attgaaatag accgaggaag actttttgac tggaaatgcg
    11521 gttataatat ctttctggat cgaaaaaacg agtggcttga gaacgaagat acccaaaaca
    11581 gagtctttga caagaaactt gccgaagagg aggtctggat tcgcaagggc attaaggcaa
    11641 gacgaacccg taacgaaggc cgggtaagag cccttatgaa gatgagagac gagcataaag
    11701 ccagaagaag tgctcagggt aacgctaaaa tagaagtaaa tcaggcatct caatcgggca
    11761 aaatggttgt agaggccgaa aacatcactt tcggttatga caaaacactt atagatgatt
    11821 tttccatacg tattgttcgc ggtgataaaa ttggaattat aggagccaac ggatgcggca
    11881 aaagcaccct gatcaggata attctggggc tgttacccgt acaaagcggt tctgttcgga
    11941 caggagcaaa tcttgaaatc gcctattttg accagcttag gaatcagtgt gatgacaact
    12001 taacagttat tgataatgtt tctaacggct gcgaaactgt caccattaat ggacgtgacc
    12061 gtcatataat cagttacctt caagattttc tgtttactcc cgaaagagct cacgaaaaaa
    12121 gtggcgtact atctggaggt gaaaaaaacc gcctgctact tgccaaactt ttcaccaaac
    12181 cagcgaatgt gctggtactt gatgaaccga caaacgacct tgatatcgaa acggtagaac
    12241 ttctggaaga acttatcctc gattttaacg gcaccgttct tatggtaagt cacgaccgtt
    12301 cgtttttaaa caatacggcg acagatataa tcagttttga accagatggt aaacttgttt
    12361 gctgcgccgg cggttacgat gactacctaa gccaacgcac tcccttaaac acgattgatg
    12421 ttaaaaaaac agaatcagaa accacaaagg caaaacccaa aagtgaaaaa ataaaactca
    12481 gttacaaaga aaacatcgaa atagagaaac ttcctcatga tattgaaaag cttgaaaata
    12541 aaatagaaga tctgcatgca aagcttggtg atcccgagct ttacaagacg aatggagatt
    12601 ccgtaaatca aatcaaggat gagttggagt cgctggaagt agagcttgaa acaaaatttt
    12661 cacgctggga agagttggaa gagaaaaggc tgcgtgccga aaccgaaagc tgatataata
    12721 accatttaaa tatctctcaa tcgatatcaa cccgtacaat agtatatttc tcattatcag
    12781 gatctacttc aattttaaga taatgacggc ctattccatc atatttttca ggcatggcgc
    12841 ctccgcctcc cgaaatacga caatcgatac cggcaagagt atacttgtaa tatgaatgaa
    12901 tatggccgaa aagcagcagg tctgtaccgt tttccccaag aaggcttaac aatttatacg
    12961 cctcgtttct gcttgaaaaa gatcctcccc ttgttccgga aggatcaaca atcggatagt
    13021 gggtcaatac aattttcaga ccatcggaaa gaccaagcca gccttcaaga tcatcgtaca
    13081 attcatgata aagagcaccc gacgaagagt cgagaaagac aaatagcgta tctcgataaa
    13141 caaagcgaaa agatgttctt ccgaaaacct gatgccaata cttgtattgt gaatcaaaag
    13201 agtcgtggtt acccatggtt gtatagagtg gcactgcaag gtattccaac tcttttataa
    13261 atcttttaat ctcacttttc gttccgttag aggtaagatc acctgtggac aatacaaact
    13321 gtaaatcatc atcactatta atcacttcaa aaatatctga gacctctgaa actgctcttt
    13381 gaacatcaga aaaaaccgcg aaaaccggat cttcttctgt atttttaaag tagatactga
    13441 tttttttatt aacaggaact tcacactcga ggctatattt gataccgttt tgattttcac
    13501 ttaaaattgt taccgaatca tcggataaag cgtaagtatt tctaacagaa ttagaaagca
    13561 ctatttgcca cgccgaaatt cccgatttac ccggatctat cacaagttct atttgcggcg
    13621 cgctctgatg aatttccagt tttcccggct caatgttttt tataactccg agccccgaaa
    13681 caacttcgac ttgcgctccg tctacagaag ccttaccaac ttttttatca agctcataat
    13741 tttcattttg aggcacatca cacgacagaa gcgccagaat aacaaacagt atagaatatt
    13801 ttgtcattaa atctccaata tgtaaatatg acttttggca atccaataag atttattaaa
    13861 aacgtttaaa acgataattt tccacttaaa gaatagaaac tagccgttcc aagaaccgct
    13921 tttaaactta ttccagtatt ttgccatgga taaaccgtaa ctgaagctcc aacatgtccc
    13981 aagaatccgc tcggcaatcc accatagaag gtatcacgcc tgtggttata gaacagatag
    14041 atatcggctt tcgaatctga aagatctcct gtaagaaatc caacccctgt ctcgaaaaga
    14101 atttgattag acgtaatcgc cggaagagtc tcatcaaaat cgaaatagac ccattcccta
    14161 ttgaaaccga cttcataaac gaaatagagc cctctcgtcg ttttccacaa agatccactg
    14221 ttgattacag ttctaaacga tatatccgtt gagtacttgt aaaacccgtc actgttaaaa
    14281 tcgtaatatc cggcacgaac aatagcatcc gatttaaaaa atgtagcgta attctcaaga
    14341 agatgcccct tttgcccggc aaatctgtat tttgccgata gttgataatg ccttgtatca
    14401 tcatctatgg cactgtttat cattggagat ataacgaatc ttttataatg ataatcgaat
    14461 cctgctttcg ccaaaagaga ataatcaaaa gccggatcgt ttgtatattc caaaccggca
    14521 ttcaaagtta ctgtcttgtt tttatccgta aaccagtcac cttccgaacg gtatcgcccg
    14581 attgtaacac cgtaaaagtc ggcgataaaa ggtaaaacca gaaacgaaac cccaaaagcc
    14641 gcgaaaggca ccaacggacc agtataatca cgaggtgccc caccatctgc aagaatataa
    14701 cccgatcccg ccagcatcaa caagcctacc ccttcgataa gaagaagctc acctgccgtt
    14761 tcgcgctcac ctgccacaaa atgaccggct ccatgtaaaa ctgaccctgg aactacagcg
    14821 gcaacaggtg gcaaaatcac ctctttataa gaagactgtt cttcagcaga gagtgcggta
    14881 tctaaattca gcaataacaa gagcagcaga taaattacga gccttttaaa aagcatttaa
    14941 ttaaacctat tcattgagtt ccattattca gtaattattt taacgtctac aaaacaatct
    15001 attcaatcga aaagtacatc tttttttcat tacaaaatgg tattttttta gacctttttt
    15061 atacctcttt ttttcaagat tattatttta caatagtgac atctccattc cagaataata
    15121 tctgcgacgt taacgataaa atgtaacaca tgtaacattt tttttgaatc tcattaaaac
    15181 atcttgactt aaataacagc tgtaacctat ttaaatacag gtaacgcgat attttaccag
    15241 aaaaccagat atgacgcgtt aatgccatat ctaattattt gagaagctgg aggcctcaga
    15301 tgaattattc ctttttaaaa actacactat tgcgcaaacc agtactgttt gtacttttaa
    15361 catctgtttt tatttcatgt tcacgtgatc cattaaaaga atatgattct caaattgaaa
    15421 ctgatcttat gcctattaag gaattttaca aagatgactt tccgattggt gccgcgattg
    15481 aaccttttca ggtcgaaaag gcggagggag ctcttcttcg ttatcactat aacagcataa
    15541 cctgtgaaaa cgcaatgaaa ttcaagtcga tacacccgaa acaaaaggtt tacaacttca
    15601 aggccgcgga caagattgcc gagtttgcaa aacgctataa catgaaaatg cgcgggcata
    15661 cctttgtatg gcatcaccct gctgaacagg tattctggat ttttttaaac aacgatggtt
    15721 ccaaacgttc tcgagagagt gttcttgaac ttctgggtga acacatgcaa acactagttg
    15781 accgttacgg cgataccatc tacgcttggg atgttgttaa cgaggccatt gacattgcag
    15841 aacccgacaa tatgcgaaaa tcacaatggt atcactccat aggccctgac tatgtcacag
    15901 aggcttttaa acttgcacga aaagccgcac cagacgcgct tcttttttat aacgattacg
    15961 atttttttaa cccgatcaaa cgtgacgcga tttatgatct tgtta
//
LOCUS       DAOTGZ010000039        15591 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_3064
            NODE_4566_length_15591_cov_4.593847, whole genome shotgun sequence.
ACCESSION   DAOTGZ010000039 DAOTGZ010000000
VERSION     DAOTGZ010000039.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868088
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 15591)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 15591)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:22:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,785
            CDSs (total)                      :: 2,751
            Genes (coding)                    :: 2,732
            CDSs (with protein)               :: 2,732
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 12 of 19
            Pseudo Genes (internal stop)      :: 5 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15591
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4566_length_15591_cov_4.593847"
                     /isolate="AMR_MDS_3064"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..978)
                     /locus_tag="P5123_04260"
     CDS             complement(<1..978)
                     /locus_tag="P5123_04260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015335026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin attachment protein"
                     /protein_id="HRX15505.1"
                     /translation="MAKKNVDVMVTAFRDGFQSVYGARVFTDDFLPAVEASVNAGINH
                     FEAGGGARFQSLYFYCNEDAFEMMDRFRKTAGPNANLQTLARGVNVVGLDSQPRDIIK
                     MHAELFKKHGITTIRNFDALNDVNNLIYSGQCIHDAGLKHEVVVTLMSLPPGTPGAEK
                     AHTPEFYAKTLKDILDAGIPFDSVCFKDASGTSTPKTVFETIKKAKEMLPAGTPLRFH
                     SHDTAGTCISQYMAALEGGANGLDLSMAPVSGGTCQSDVISMWHALRGSDWDLGIDID
                     KMRDAEEVFKECMADYFMPPEAKAVEPLIPFSPMPGGALTANTQMLRDNN"
     gene            complement(992..1216)
                     /locus_tag="P5123_04265"
     CDS             complement(992..1216)
                     /locus_tag="P5123_04265"
                     /inference="COORDINATES: protein motif:HMM:TIGR01195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008948 - oxaloacetate decarboxylase
                     activity [Evidence IEA];
                     GO_function: GO:0015081 - sodium ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0036376 - sodium ion export across plasma
                     membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="OadG family transporter subunit"
                     /protein_id="HRX15506.1"
                     /translation="MESFIQGLEFMVIGVSVVFMFLVVMVFAMSLLRITLTQLNKYFP
                     EPEEQKAAPASGDNTQEIAVAIAAAYSMKK"
     gene            complement(1192..1950)
                     /locus_tag="P5123_04270"
     CDS             complement(1192..1950)
                     /locus_tag="P5123_04270"
                     /EC_number="6.3.4.15"
                     /inference="COORDINATES: protein motif:HMM:TIGR00121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004077 - biotin-[acetyl-CoA-carboxylase]
                     ligase activity [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biotin--[acetyl-CoA-carboxylase] ligase"
                     /protein_id="HRX15507.1"
                     /translation="MSNFRFAIKKLNTVDSTNSYALANIDMFTHNTLVYSDIQTSGRG
                     RLNRKWISDTPENLYCSVIIKPEQDYKKLPLVNFTQLFCVILAEIINDLGVTPQIKWP
                     NDILVNEKKIAGILSESSFTGSNFNGIVVGIGLNIGALPKEALDREQAVTSLYDECGR
                     EKSREEIIQKIAELYSEYYDLFCETGFSAIRDSYLSFFPFIGKEVSIENGTVNVTGNV
                     VDVSPKGELIIDNGSEGLQNIVLGDMVWKALSRA"
     gene            complement(2055..2507)
                     /locus_tag="P5123_04275"
     CDS             complement(2055..2507)
                     /locus_tag="P5123_04275"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRX15508.1"
                     /translation="MALNKKKLELEEVKENLNYQTYIFVSYLRPDFVDCILRITVNKE
                     NRVIEQLYFSSSSYGTASWINGSGVVEAHGWETEDSAAEVSYLSKHYPALLLNGEVSR
                     DCAYSVIIPDVLSHLFNGKLAEILQNTVKEKTKSSAVFDPEDYNIDTV"
     gene            complement(2508..3728)
                     /locus_tag="P5123_04280"
     CDS             complement(2508..3728)
                     /locus_tag="P5123_04280"
                     /EC_number="2.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019440928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HRX15509.1"
                     /translation="MKKIYKSKKLENVCYEIRGPVMKEAQRLEEEGFQILKLNIGNTA
                     PFGLMAPDEMIHDVILNMPKSQGYCDSKGLFSARKAVMQYYQQKGLLEPDIEDIYIGN
                     GVSELIVMTMQGLLNNGDEVLIPAPDYPLWTAAVSLSGGKPVHYICDEQSDWYPDIDD
                     IRSKITDKTKAIVVINPNNPTGSVYSKEIVEQLVKVAVENKLVLLADEIYEKIVYDDN
                     KHYSPALMTDETLVITYNGLSKSYRAPGFRAGWMVLTGLKSMAKDFIDGLDILANMRL
                     CSNVPSQYSIQTALGGYQSINDLILPSGRLGKQREVCYDLLTSISGISCVKPKGALYM
                     FPKIDVKKFGIKSDIKFAFDLLQHKKMLIVQGSGFNWPKSDHFRIVFLPDVDLLGEAM
                     SRLKEFLGSYYQEE"
     gene            complement(3860..4945)
                     /locus_tag="P5123_04285"
     CDS             complement(3860..4945)
                     /locus_tag="P5123_04285"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRX15510.1"
                     /translation="MPVVSNTNNMSSRAFLNNHIESLFIKDIKDNYDALVAVKEDRES
                     HLLVEKYWIVLNKVLNDCDAIGFGENENIRDVAIETSLLMIDAFVETIFLNNVPEADL
                     QEYCIYQIDMVMDVVKRYGIDEEYRERLAGTLTKYMRNYVDELRNLAFEEFYNYTKRL
                     GIEKYSKYVDFTMYELGIILYSNLIESYVQKFKSDPNPELRLYIEELSHLVEKAHSYE
                     FISSQIANKIVYTCVDLVEGGMEKKYFEKEVIREVTKQIIALLTKYDLMNDCFWNALT
                     DIFTIYMKMMTEKDERENFKFYWLFSQEIEILDRVGQVPDKTAGIEVKRGEASSVSKS
                     GAKKSASKSAAPAKAAKKRKSGGLREL"
     gene            5192..5923
                     /locus_tag="P5123_04290"
     CDS             5192..5923
                     /locus_tag="P5123_04290"
                     /inference="COORDINATES: protein motif:HMM:NF013348.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HRX15511.1"
                     /translation="MSKPDEIFLKKMIKHCGKTINRHNLIEADDHILLALSGGKDSLA
                     LLDIISRRRKHLPINYKLTAAFINIIGITDNVDQSKLNLFCKERDAGFISQSVDVDFT
                     QNTDPCFACSWHRRKILFEMCRKMDIKTLALGHTLDDTSNTLLMNIFFQGNISSMTPS
                     LDLFDGNLKIIRPLIETDEKNITHYCNLLSLPIQKGKCHYGKAQKRNQIDKIIKEIEK
                     THPQVRNNIYSSLSNIRSDYLPEQF"
     gene            complement(5902..6651)
                     /locus_tag="P5123_04295"
     CDS             complement(5902..6651)
                     /locus_tag="P5123_04295"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRX15512.1"
                     /translation="MKKHIGRIFIVLIFFMLFSGCDDSDGGEDDCEMPPLDCQLMPPT
                     SGSVKIFVSNGYDVKVELFEGLIGDNAVLIGEYTVSDGSFVLNNMADGEYSARAYYEV
                     EGESIAVTGGDDIYIHSEEYCAGIECWEVDDAEIDVSLDIDAFKDYLEGKEDHCFIAT
                     AAFGSLAERHVRILRVFRDRVLLENGIGRIFVKYYYRYSPPVAQVVRNSPLLRRCVRG
                     VLSPITAVIMLLFPEIEAGMAWSSIQNCSGR"
     gene            complement(6708..7235)
                     /locus_tag="P5123_04300"
     CDS             complement(6708..7235)
                     /locus_tag="P5123_04300"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRX15513.1"
                     /translation="MKNCLMLFGYFTVLFMLASFDAFAFTGGGIGGDANTKDLQLQLD
                     ASFPLNKDLGLDILPFFSFRPIAKTVFEEESGAYFQYDEYRFNIGTVLSKSFFFTEKF
                     GLFVEGGGGYSISKYSGVSVYDGNHHLFPVFRTGICFRGKLGLNILFKIGYEYLPIEN
                     VPANRGYGSILFGVF"
     gene            complement(7337..8974)
                     /locus_tag="P5123_04305"
     CDS             complement(7337..8974)
                     /locus_tag="P5123_04305"
                     /EC_number="6.3.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007413786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003952 - NAD+ synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD+ synthase"
                     /protein_id="HRX15514.1"
                     /translation="MKIAVVQLNYIVGDIRYNSAKIIDAVNNLKGKVDIAVFSELSVI
                     GYPPMDLLENRKLLEDNLNAATKIAEYAEGIAIVFGFAYKEKGVLYNSAALAIDKKIA
                     GIQHKGLLPTYDVFDEHRYFSEFDCFEPFEYKGKKIGLTICEDIWNSSKVNKMFMDNR
                     SYDFDPFDKLVKRGCDFIINISASPFVSGKGEHRISFISGLSRNSGIPVVYCNQVGGN
                     DSLIFDGTSFVTDKDGNIVSLAKRFEEDVIIADISGESSIKPVNFSFNSLDNLRSALV
                     LGVRDYVRKCGFSKVIVGLSGGIDSALTAVIAVDALGAENVMGITMPSRYSSEGSVSD
                     SVKLAENLGITIETVPIKMLHDQFLNTLSPLFSGYKEDVTEENIQARIRGTLLMAMSN
                     KFNALLLTTGNKSELAMGYCTLYGDMSGGLAVISDLPKTTVFELSRYINKEQEIIPWN
                     TIKKPPSAELRENQKDQDSLPPYDQLDEILRLYISERYSCDEIISKGFEPKLVNHILK
                     TVDRNEYKRRQAAPGLKVTSKAFGTGRRIPVVQRTEH"
     gene            complement(9108..9719)
                     /locus_tag="P5123_04310"
     CDS             complement(9108..9719)
                     /locus_tag="P5123_04310"
                     /inference="COORDINATES: protein motif:HMM:NF012655.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HRX15515.1"
                     /translation="MPAKGQKRKQQIMDVAKEMFIKNGFQSTHIGQVCDTLEIARGTV
                     YQYFGNKREILYAVLESVEEKFDDIFDVDDLDFFLEGKPGKELMHEFVSNRIASCIKA
                     ILSEPIVIKLMYKEIFGIDEDVVNHVNNFVAYINRILVRDFKVFKEKGFYKSDISEEV
                     SAGMLIGGVLLLLHEYEVRGENILNREIIDSIAKNFLFGVVSS"
     gene            complement(9719..9871)
                     /locus_tag="P5123_04315"
     CDS             complement(9719..9871)
                     /locus_tag="P5123_04315"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRX15516.1"
                     /translation="MSRELRERADIISRELRELLTPITQEIRVRKAESFEKSSITYPG
                     FSKGDR"
     gene            10098..11063
                     /locus_tag="P5123_04320"
     CDS             10098..11063
                     /locus_tag="P5123_04320"
                     /inference="COORDINATES: protein motif:HMM:NF013567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HRX15517.1"
                     /translation="MKKIRCAIAGLGRIGCELENDPKREKPATHAGAVNDHPDTIICA
                     GADTDPEKRNNFTLNWGVQNVYESLNELLSNEPVDIVQIATPPETHLQLVKTAVDYSV
                     PVIILEKPVASTLDEARQIVTIEKNSKSKIVINHERRYSLQYRHVKHQIESGQIGALY
                     SINAKLCIGSKRTVADMLYDDGTHMIDLLSHLTGEALVPYNVNLAHNRTVAFVYGKCG
                     DTPVTLEVGAGRDHLIFELDFSFSNGRIRVGNGYFEEYCSQVSPFYSNYRSLVKNSTH
                     FPKTEYFKLMFKEAVDIYKNEKEYTVSSTEDGLSAITVIDQILNW"
     gene            11263..11625
                     /locus_tag="P5123_04325"
     CDS             11263..11625
                     /locus_tag="P5123_04325"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HRX15518.1"
                     /translation="MDKPSILVVEDEIIIRQMLVNQLKKLDCNVIADVSSGEEAVEIA
                     NFPDKIDLICMDINLSGSIDGIEAAEIISSQRDVPILFLTAYELPVSIDKKISNYGYI
                     GKPIPMEKLRDFIFDIKG"
     gene            complement(11626..12936)
                     /locus_tag="P5123_04330"
     CDS             complement(11626..12936)
                     /locus_tag="P5123_04330"
                     /EC_number="4.6.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012437.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009975 - cyclase activity [Evidence IEA];
                     GO_function: GO:0016849 - phosphorus-oxygen lyase activity
                     [Evidence IEA];
                     GO_function: GO:0032555 - purine ribonucleotide binding
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate/guanylate cyclase domain-containing
                     protein"
                     /protein_id="HRX15519.1"
                     /translation="MRRIIQNGLPTSTGVLLLQKERQGLKITMVFRIMWFLALLAGHL
                     FTYQSQLELVLVASILFIAVISTVIFMFMLRKESLIRVAGTSGALMDVILIGLLPFIW
                     YISVGAVNTTPAFMVKGALSVTIFLFMIVNSLALRPRYPVIIMTGASMILVLLFVIIV
                     LNPQTVYSYSFLDIFMGEAFSPIYFVLTNICTVILAGIFLSLITAAARRTILEAVTLS
                     RANSQMERYFSPNVVGSIINSEETFLKPGGRLQKVAVMFCDIRDFTSISESMKPEELL
                     LMLSEYQSRMVKAIFHHGGTLDKFIGDGIMATFGTPEVSDDDALRAVKAGIEMKKELS
                     SLNLSRMEQGLKPIRQGIGIDYGPVVAGNVGTESRLEYTVIGDTVNSASRIEGACKET
                     GENFIISKSVKNCLDETIITKELDEILLKGKKKKIGLYAVIIDG"
     gene            13196..13891
                     /locus_tag="P5123_04335"
     CDS             13196..13891
                     /locus_tag="P5123_04335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010932468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc metallopeptidase"
                     /protein_id="HRX15520.1"
                     /translation="MLYFGLDITYWLIMIPFFLFALWAQSRVKAAYKKYSRIAIYNGL
                     TGAQTAQQILLNNRITDVRVERGSGLLSDHYNPSKKVIRLSPGVYDSNSVAAVSIAAH
                     ETGHAIQHAKAYSPLALRSVLAPTASIGSNFAFIIIFAGLLINMAGLIKFGIALFAIV
                     VFFQLVTLPVEFDASARAKRALVSYSMVNNTELKGVNTVLNAAAMTYVAAAASAIAQL
                     LYLLLRSGFLGDD"
     gene            complement(13987..15402)
                     /gene="pyk"
                     /locus_tag="P5123_04340"
     CDS             complement(13987..15402)
                     /gene="pyk"
                     /locus_tag="P5123_04340"
                     /EC_number="2.7.1.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020530525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004743 - pyruvate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate kinase"
                     /protein_id="HRX15521.1"
                     /translation="MFKKKTKIVATISDMRCDVDFIRSLYNAGMNVVRLNTAHQSPHE
                     TLSVVKNIRTVSEKIAILVDTKGPEIRTTAADSGISVQADDIVYLAGNPDEKCSAECI
                     NVSYSSFVRDVPVGNRILIDDGDLELLVEEKEGDRLKCRVMNSGVIKGKKSINVPGVS
                     LDLPSLSDRDREYIEFCIENDIDFIAHSFVRRKEDVIAIQRILDAKNSSVKIIAKIEN
                     QEGVDKIDEIIDYAYGVMIARGDLAIEIPAEKIPYTQKTIIKKCLSRRKPIIVATQML
                     HSMIDNPRPTRAEISDVANAVYDRTDALMLSGETAYGKYPLESVQMMSKIACEVEVGL
                     DRYNTTTQEKVFNEITAYLAKAAVDAAAELNTTAIVIDTLTGRTARSISVYRGSALVY
                     AECYSKRTVRELALSYGVYANYLDVQDSHRGFIENALGYILERKAISREDRIVIVAGN
                     FGAARGASYIEIGTPNELLAK"
BASE COUNT         4933 a         3499 c         2506 g         4653 t
ORIGIN      
        1 attattatca cgcaacatct gagtatttgc agttaaagct ccacccggca ttggtgaaaa
       61 tggtataagt ggctcaacag ctttggcttc cggaggcata aaatagtccg ccatgcactc
      121 tttgaaaacc tcttcggcat cacgcatctt gtctatatca atgccaagat cccaatcaga
      181 accccgtagt gcatgccaca ttgaaataac atcactctga catgttccac cagatactgg
      241 ggccatcgaa agatcaagac cgtttgcacc accttcaagc gcagccatat actgcgaaat
      301 acatgtaccg gcagtatcgt gcgaatgaaa tcgaagagga gttccagcag gaagcatctc
      361 tttggctttt tttattgttt caaaaactgt tttaggagtc gaagtaccgg aagcatcctt
      421 aaaacataca gaatcaaaag gaataccggc atcaagaata tctttcaacg tttttgcata
      481 aaactcagga gtatgagctt tttctgcacc aggtgttccc ggaggcaagc tcataagtgt
      541 aacaacaacc tcatgtttta aacccgcatc atggatacac tgtccagaat agataaggtt
      601 gtttacgtca tttaatgcat caaagttacg aattgttgta attccatgtt tcttaaacag
      661 ttccgcgtgc attttgataa tatcacgagg ctgagaatca agcccaacaa catttactcc
      721 tcgggcaaga gtctgaagat tagcgttagg acccgcagtt tttctaaacc gatccatcat
      781 ttcaaaagca tcttcattac aatagaaata gagcgactga aaacgtgctc caccacctgc
      841 ttcaaaatgg ttaatacctg cattaaccga agcctctaca gctggaagga aatcatctgt
      901 aaatacacgc gcgccgtata ccgactggaa gccgtcacga aacgcggtta ccataacatc
      961 tacattcttt tttgccactt tgtatacctc tttacttttt catcgaatat gcggccgcaa
     1021 tagcaacagc aatctcttga gtattgtctc ccgaagccgg agcagccttc tgctcttcag
     1081 gttcaggaaa gtacttatta agttgtgtta atgtaattcg aagtaaactc atcgcgaaaa
     1141 ccatcacgac aagaaacata aaaacgacag aaacacctat taccataaac tctaagccct
     1201 ggataaagct ttccatacca tgtcccccaa tacaatattt tgtaaacctt ccgatccatt
     1261 atcaattatc agttcaccct ttggcgaaac atcgacaaca ttaccggtta cgttcacagt
     1321 accgttttct atactaacct ctttgccaat aaaaggaaaa aaggaaaggt aactatctct
     1381 gattgctgaa aaacctgttt cacaaaacag gtcataatac tcactgtaaa gctctgctat
     1441 cttttgaatt atctcttcac ggcttttctc tcttccacac tcgtcataaa gagacgtcac
     1501 tgcctgctca cggtcaagag cttctttggg caaggcccca atattaaggc ctattcctac
     1561 aacaatacca ttgaagtttg atcctgtaaa tgatgattca ctcaaaattc cggcaatctt
     1621 cttctcattt acaagaatgt cgtttggcca ttttatctgt ggtgtaacac caagatcgtt
     1681 tattatctcc gcaagaatca cgcaaaacaa ttgggtaaaa tttactaatg gaagtttttt
     1741 ataatcctgt tccggcttaa ttattacaga gcaatagagg ttctcaggag tgtcagaaat
     1801 ccatttgcgg tttaaccttc cccgcccaga cgtctgaata tcagaataaa caagggtatt
     1861 gtgagtaaac atgtcaatat tggccaatgc atagctattt gtagaatcga ccgtatttag
     1921 tttttttatc gcaaacctaa aatttgacac gattattacc cctcaacaat cataactata
     1981 aaacaacttt tttttgtcaa gtgggtaact gtaattatta taagaatagt gctatattaa
     2041 cggggaaata atatttaaac agtatcaata ttataatcct caggatcaaa tactgcactg
     2101 ctctttgtct tttcttttac agtattttgc aatatttcgg caagctttcc attaaaaaga
     2161 tgtgacaata catctggaat aatcacggaa taggcacaat cacggctcac ctctccattt
     2221 agcaatagcg cggggtaatg tttagacaaa taagacacct cagccgccga atcttcagtt
     2281 tcccaaccat gagcctctac cacacctgaa ccgtttatcc agcttgccgt accgtagctg
     2341 cttgaagaga agtaaagctg ttcaataaca cgattctcct tattaacagt aattcttaat
     2401 atgcaatcta caaaatcagg acgtaaataa gatacaaaaa tgtaggtttg gtaatttaga
     2461 ttctctttta cttcttctaa ctccagtttc tttttattta acgccaccta ctcctcctga
     2521 tagtaactgc ctaaaaattc ttttagccgc gacatggcct caccaagtaa atcgacatcc
     2581 ggaagaaata caattcgaaa atggtcagat ttcggccaat taaagccact cccctgtaca
     2641 ataagcattt ttttatgctg caatagatcg aaagcaaatt tgatatcact ctttattcca
     2701 aattttttca catcaatctt tggaaacata tacaatgctc cctttggttt aacacagctg
     2761 attcctgaaa tagaggtcag caaatcataa cagacctccc gttgcttgcc aagacgaccc
     2821 gaaggcaaaa taagatcatt aatactttgg taccctccca aagccgtctg tattgagtat
     2881 tgagaaggaa cattgctaca taatctcata tttgccaata tgtccaaccc atcaataaaa
     2941 tctttagcca tgctctttaa accggtcagt accatccagc cagctctaaa acccggcgcc
     3001 ctgtacgatt ttgacaatcc attgtatgtt attacaagcg tttcatcggt catcagcgcc
     3061 ggtgaatagt gcttgttatc atcatacaca atcttctcat atatctcatc agccaaaagt
     3121 acaagcttat tctctactgc cacttttaca agctgttcaa caatttcttt tgagtagaca
     3181 ctacctgttg gattattggg atttataaca acaatagcct ttgttttatc ggtaatctta
     3241 gaacggatat catcaatatc gggataccag tctgactgct catcacatat ataatgaacc
     3301 ggtttgccac ctgaaagaga gacagctgcc gtccataaag gataatcggg tgccggaata
     3361 agcacctcgt ctccgttatt aagtaaacct tgcattgtca taacaattaa ttcacttacg
     3421 ccgtttccaa tgtaaatatc ctcaatatcc ggctcaagaa ggcctttttg ctgataatac
     3481 tgcatcacag ctttacgagc tgaaaacaat ccttttgaat cacaatatcc ttgtgattta
     3541 ggcatgttta atattacatc gtggatcatc tcatcaggcg ccatgagtcc aaaaggcgca
     3601 gtgttaccta tattaagttt tagaatctga aatccctcct cttcgagcct ttgagcctcc
     3661 ttcataacag ggcctctaat ctcataacaa acattttcca acttttttga tttataaatt
     3721 tttttcattt aaattccacc tcgaaatatt gctgaatacc ttttaactat ttctgactaa
     3781 cgattaccct tataaaatta cggcataaaa aaagacccct ctacggagcc tttttcgtat
     3841 ctataaattg ttttatagtt tacagctcac gcagaccacc ggattttcgt ttcttcgcgg
     3901 ctttggccgg agcagcagat ttagatgcac tttttttcgc gccagacttg gaaacagacg
     3961 aagcttcccc acgcttaact tcgatacccg cagtcttatc cggaacctgt ccaacacggt
     4021 caagaatctc gatttcctgt gaaaagagcc agtaaaactt gaagttttca cgctcatctt
     4081 tttccgtcat catcttcata tatatggtaa aaatatcggt cagagcgttc caaaaacagt
     4141 cattcataag atcgtacttt gttaaaaggg caatgatctg tttggtaacc tcgcgaataa
     4201 cctctttttc gaaatatttt ttctccatgc caccttctac gaggtcaaca caggtataca
     4261 caattttatt agcgatctga gaagaaataa attcgtaaga atgcgccttt tccacaagat
     4321 gactcaactc ctcgatgtaa agtcttaact caggattagg atcagactta aatttctgaa
     4381 cataactctc gataaggtta gaataaagta ttattccaag ctcatacatg gtaaaatcaa
     4441 catactttga gtacttttct ataccaagtc gcttggtata attgtagaac tcttcaaatg
     4501 ccagatttct caactcgtcc acatagttcc tcatatattt tgtgagtgta cccgcaaggc
     4561 gctcgcggta ctcttcatca atgccatatc gtttaacaac gtccataacc atatcaatct
     4621 gatagataca atactcctgc aaatcagcct caggcacatt gtttaagaat attgtttcaa
     4681 caaaagcatc aatcattagc aaagaggttt ctatcgcgac atcacggata ttttcgtttt
     4741 caccaaatcc gatagcatca cagtcattta ataccttgtt cagtacgatc cagtattttt
     4801 cgacaagcag atgagattcc ctatcttcct tgactgcaac aagagcatca tagttatcct
     4861 tgatatcctt gataaaaaga ctttcgatgt gattgttaag aaaagccctc gaagacatat
     4921 tatttgtatt tgaaacgact ggcatttttt tgctccttgg ttagagtctt gatctattat
     4981 tacaacatcc ctttaaaaaa atagtgacca taagaacatc aactgtatat ttgacagcaa
     5041 agctatcttt ttaaaatttc aggtgtcctt tataagattc cctgacgaac aatggtattt
     5101 tagatatcca taggtaatga aacaggactt tttatcaact gtcaagtttt ttacatcaag
     5161 atagctatta attttaaggg tactgtttaa aatgagtaaa ccggacgaaa tatttctcaa
     5221 gaaaatgata aaacattgcg gcaaaacgat aaacagacat aatcttatag aagcagatga
     5281 ccatatactg ttagccctat cgggcggaaa agattcactc gctctactcg acataatttc
     5341 ccgccgccgc aaacatctac cgattaatta caaattaaca gctgccttta ttaatataat
     5401 cggtattaca gataacgtag accagtcaaa acttaatctt ttttgcaaag aacgggacgc
     5461 cggttttatc agccaatctg tcgatgtcga tttcacacag aataccgacc cctgtttcgc
     5521 ctgttcatgg catcgtcgca agatactgtt tgaaatgtgc cgaaaaatgg atattaaaac
     5581 actagctctc gggcatacgc tagacgatac atcgaacacg cttcttatga atattttttt
     5641 tcagggaaac atctcctcta tgacaccttc acttgatctc tttgatggaa atctaaaaat
     5701 aatacgccct cttatcgaaa cagacgagaa gaatatcaca cactactgta atcttctctc
     5761 gctaccgatc caaaaaggga aatgtcacta cggtaaagcc caaaaacgca atcaaattga
     5821 taaaataatc aaagagatcg aaaaaacaca tccccaagtt cgaaataaca tatattcatc
     5881 tctttcaaac atccgctcag attatctccc agaacaattc tgaattgacg accatgccat
     5941 acccgcttct atttccggaa acaaaagcat gataacagcc gttatgggcg acaacacccc
     6001 tctgacacag cgtctaagca aaggtgaatt cctaacaact tgagcaactg gaggcgaata
     6061 acggtaatag tacttaacaa aaatccttcc aattccattc tcaagaagca ctcgatctct
     6121 gaacacccgc aaaattctga cgtgtctttc tgccaacgac ccgaaagcgg cagtagcgat
     6181 aaagcagtga tcttcttttc cttccagata atccttgaag gcatcaatat caagagaaac
     6241 atcgatctcg gcatcatcta cttcccagca ctctattccg gcgcaatact cttcactatg
     6301 tatataaata tcgtctccgc cggtcacagc aatgctttct ccttccactt cataatatgc
     6361 acgagccgag tattcaccat cagccatatt attcaaaaca aaactaccgt ctgaaacagt
     6421 atattcaccg attaataccg cattatcgcc aatcaacccc tcgaaaagct ctactttaac
     6481 gtcgtaaccg ttacttacaa aaattttaac cgaaccgcta gttggaggca tcaattggca
     6541 atcaaggggc ggcatctcac aatcatcttc accgccatca ctgtcatcgc atccggaaaa
     6601 aagcataaaa aaaatcagaa caatgaaaat tctaccaata tgttttttca atttaaaacc
     6661 tcctaaacaa ataaacagac gtaaattttc cctttggcta ataatcgtca aaaaactcca
     6721 aatagaatcg atccgtatcc tctgttagca ggcacatttt caattgggag atattcatat
     6781 cctattttaa ataaaatatt cagtcccagt ttacccctaa agcatatccc ggtacgaaaa
     6841 accgggaaaa gatggtgatt gccatcataa acagaaactc cactatattt actgattgaa
     6901 taaccgccac ccccttcaac aaacaggcca aatttctctg taaaaaagaa gctttttgaa
     6961 agaacagttc caatattgaa acgatactca tcatactgaa agtatgctcc ggactcttct
     7021 tcaaagacag ttttggcaat agggcgaaat gagaaaaaag gtaagatatc caagcctaaa
     7081 tctttattta acgggaaaga tgcatcaagc tgaagttgaa gatcctttgt attagcatca
     7141 ccacctatac caccgcctgt aaaagcaaag gcgtcaaaac ttgccagcat aaacagaaca
     7201 gtaaaatatc caaaaagcat aaggcaattt ttcattttag cccccattgt ccaaatgaga
     7261 cacaaatatt gatcctaata taaagcacct gaccgtaaaa tctacaaatt caattcaagt
     7321 aatcaaactt tttttattaa tgttctgtcc tctgtacaac tggtatgcgt cttcctgtac
     7381 caaacgcctt cgaggtaacc tttagtccgg gagctgcctg ccttctttta tactcgtttc
     7441 gatcaaccgt cttaaggatg tggtttacca acttcggctc aaaacctttg ctgattatct
     7501 catcacaact ataacgctca ctgatataaa gacgtaatat ctcatcaagc tgatcataag
     7561 gtggcaaaga atcctgatct ttctgatttt cacgaagttc ggccgagggg ggttttttaa
     7621 tcgtattcca gggaatgatc tcctgttctt tatttatata tcggctcagc tcaaaaacag
     7681 tggtttttgg aagatccgat atcacagcaa gaccgccgct catatcgccg taaagagtac
     7741 aataacccat tgcaagttcg gatttatttc cggttgttaa aagaagtgcg ttaaacttgt
     7801 tgctcattgc cataagcagc gttccccgta tgcgagcctg aatattctct tcggtaacat
     7861 cctccttgta tcctgagaac agaggggata gcgtattcaa aaattgatca tgaagcattt
     7921 ttataggaac agtttctatt gtaatcccaa gattttcagc taatttaaca gaatcactca
     7981 cgctgccctc agaagaataa cgcgaaggca ttgtgatccc cataacattt tcggcaccga
     8041 gagcatctac cgcaattact gctgttaatg ccgaatcgat cccaccggat aatccaacta
     8101 ttaccttact gaaaccacat tttcttacat aatcacgaac tcccaaaaca agcgccgacc
     8161 tcagattatc aagagaatta aaactgaaat ttacaggctt gatagaactt tcaccggaga
     8221 tatccgcgat aataacatct tcttcaaatc tttttgcaag agaaacaata ttaccatctt
     8281 tatcagtaac aaatgatgta ccatcaaaaa tcagcgaatc atttccccct acctgattac
     8341 agtaaacaac cgggatcccc gaattacgtg aaagccctga tataaaagaa attctatgtt
     8401 ctccctttcc cgatacaaag ggagaggctg aaatatttat tataaaatca caacctctct
     8461 ttacaagttt atcaaaaggg tcaaaatcat aactacgatt atccataaac attttattga
     8521 ccttggaact attccagata tcctcacaaa tcgttaatcc tatttttttt cctttgtact
     8581 caaacggttc aaaacagtca aattccgaaa aatacctgtg ttcatcaaaa acatcatagg
     8641 ttggcaacag ccccttgtgc tgaatacctg cgattttttt atcaatagcc aaagctgcac
     8701 tgttatatag aacacccttt tctttatatg caaaaccaaa aactatcgct atcccctctg
     8761 catactcggc aatttttgtg gcagcattta aattgtcttc caacagtttg cgattttcaa
     8821 gtaaatccat gggtggataa cctattacag ataattctga aaaaacagca atatccactt
     8881 tgcctttaag attatttaca gcgtcaatta tctttgcgct gttatacctg atatcaccga
     8941 cgatatagtt taactgaaca actgcaattt tcatatttct atccattaag aaactatgta
     9001 agataaactc attaaaaagg agggtaaaac cataatacgg aaggtgaagg tcggagcatg
     9061 actattgcaa acataaaagg atcagcttac tacaaatgat ctgatactta tgaagagacg
     9121 actccaaaaa gaaagttttt agcaatagaa tcaataatct cacgatttaa tatattttcg
     9181 cctctaacct cgtactcatg caaaagcaaa agcaccccac ctataagcat accagctgaa
     9241 acctcttcac taatgtcaga tttatagaat cctttctctt tgaaaacttt aaaatctcga
     9301 acaagtattc tatttatata tgctacaaaa ttattcacat gattgacaac atcttcatct
     9361 atgccaaaaa tttccttgta catcagttta attacaatcg gttctgacaa aattgccttt
     9421 atacacgagg ctatcctgtt actgacaaat tcgtgcatca gctcttttcc aggtttacct
     9481 tcaaggaaaa aatcaagatc atccacatca aatatatcat caaatttttc ttcgacagat
     9541 tccaaaactg cgtacagaat ttccctttta ttaccaaaat attgataaac agtgcctcgt
     9601 gctatctcaa gagtatcaca aacctgacct atatgagtgc tttgaaagcc atttttaata
     9661 aacatctctt tggcaacatc cattatctgc tgctttcgtt tttgtccttt ggcaggcatt
     9721 atctatctcc cttgctaaaa ccggggtaag ttattgaaga tttctcgaag gactccgctt
     9781 ttcttacccg tatttcctgt gtaattggag ttaacagttc tctcaattca cgggaaataa
     9841 tatcggctct ttctctcagc tctctactca tatacctacc tgctgacctt atatagtcaa
     9901 caatttattt gttcaatttt gttaaaatgt ttacttaatt ttataaatat taccatttta
     9961 tggcaaattt cattccagtt atataatatg attatcggac tccacaaaat gattgttcat
    10021 aatattttta gctctcctac tttatttcat tatccgtttt ttccgatact aattataaac
    10081 ggaacattaa accaaacatg aaaaaaataa gatgtgcgat agccggtctc ggccgaatcg
    10141 gttgcgaact tgaaaatgac ccgaaacgag aaaaaccggc cactcatgcc ggagccgtta
    10201 atgatcaccc cgatactatt atttgcgcgg gagcggatac tgatccagaa aaaagaaaca
    10261 atttcacttt aaactggggt gtccagaatg tgtatgaatc tctcaatgaa cttctatcta
    10321 atgaaccggt cgacattgta caaatagcaa ctccccctga aactcatctt caacttgtaa
    10381 aaactgctgt tgattattca gttccggtaa taatactcga aaaacctgta gcatccactc
    10441 tagatgaggc tcgacagata gttactattg aaaaaaactc caaatcaaag atagttatta
    10501 atcatgaaag acgatacagc ctacaatatc gtcatgttaa acatcaaatt gaatctggtc
    10561 aaattggtgc actctattca ataaacgcaa aattatgtat aggcagcaaa agaacagtcg
    10621 ccgacatgct ctatgatgac ggcacacaca tgattgatct cttatctcac cttacgggag
    10681 aagctcttgt tccatataat gtaaatctcg cacataaccg gactgtagct ttcgtttacg
    10741 gtaaatgcgg tgatactcct gtaacactag aggttggtgc tggtcgtgat catctcattt
    10801 ttgaactgga tttctcattc tcaaacggtc gtatcagagt cggtaacggt tactttgagg
    10861 aatattgtag ccaggtgagt cccttctaca gcaattaccg gtctctggtc aaaaatagca
    10921 cacattttcc caaaaccgaa tattttaaac ttatgtttaa agaggcagtc gacatttaca
    10981 aaaatgagaa agagtatact gtttccagta cagaagacgg gctttcggca attacggtta
    11041 tagatcagat tctaaactgg taattgtcgg catgttttct tgattttact ggcaaaataa
    11101 ctacccaagg tatacccgag aaatttcggg taacaacatc aataaacaga tatgtctttg
    11161 cagcctaaaa aattattcat gatattttat tgcctttttt gtagatataa gctataatat
    11221 attgaacggc tttttacata tagacattac aacgggaaac taatggataa accaagtata
    11281 ctagtagtgg aagacgagat aataatccgc caaatgctcg taaatcagct gaaaaaactc
    11341 gattgtaacg taatagccga tgtctcttcg ggcgaagagg ctgtagaaat tgctaatttc
    11401 cctgataaaa ttgatctgat ctgtatggat ataaacctaa gtggctcaat tgacggcata
    11461 gaggctgcag aaataatcag ttcgcaacgc gatgttccaa tactttttct tacagcatat
    11521 gagctaccag tatctatcga taaaaaaata agtaattacg gttatatagg caaaccaatc
    11581 cccatggaaa aacttcgtga ctttattttt gacataaaag ggtaatcagc catcgataat
    11641 taccgcataa agaccaattt tcttcttttt accctttaat aaaatttcat caagctcttt
    11701 cgtaataatt gtctcgtcca aacaattttt tactgatttc gatattataa aattttcacc
    11761 tgtctcctta cacgcccctt caatacggct agctgagttc acagtatctc caataactgt
    11821 atattccagt ctgctttcag ttccaacatt tcctgctaca acaggaccat agtctatacc
    11881 tatcccttgc ctgatcggtt taagcccctg ctccattcta gataaattaa gcgaggagag
    11941 ttcctttttc atctcgatac ccgccttcac agcacgcaag gcatcatcat ctgacacctc
    12001 cggtgttccg aaggtggcca ttattccgtc acctatgaac ttgtccaacg tgcctccatg
    12061 atgaaagatt gctttaacca tacggctctg atactcagat aacatgagta aaagttcttc
    12121 cggtttcatg gactcactaa ttgaagtaaa atcccttata tcgcaaaaca taactgctac
    12181 tttctgcaaa cgtccgccag gtttcaaaaa ggtctcctcg gaattgatga tgctgccaac
    12241 aacattggga gaaaaataac gctccatttg cgaatttgcc ctagacagag ttacagcctc
    12301 tagaatagtt ctgcgtgccg ctgcagtaat aagtgataga aaaattccgg ctaatatcac
    12361 agtacatata ttggttaaaa caaaatatat tgggctaaat gcctcgccca taaaaatgtc
    12421 cagaaaactg tagctgtata ctgtctgggg gtttaagact attatcacaa aaagaagcac
    12481 aagtatcatt gaagcccccg tcattattat tactggatac ctaggacgta aagcaagact
    12541 gttgacaatc ataaacaaaa agattgttac ggacagtgca cccttaacca taaaggcagg
    12601 cgttgtattc actgcaccta cagagatata ccaaataaaa ggaagtaaac cgatgagtat
    12661 tacgtccatt agcgcaccac tagttccggc aacacgtata agtgattctt tgcggagcat
    12721 aaacataaaa atcacggtcg agattacggc aatgaacaaa attgaagcaa caagcactaa
    12781 ctcaagttga ctctgataag taaaaagatg tccggcaagt agagcaagaa accacataat
    12841 acgaaacacc attgtaattt taagcccctg cctctccttt tgcagaagca acacaccggt
    12901 acttgttggt aaaccgtttt gaattatcct acgcatacac tatctccgtg acaaactttt
    12961 gatgaacaat agtaacatta acaccgtaaa atagtctatt aacaattaga cgcaaacaag
    13021 gttttcaagg cttaacaaac aaatttttta gaaattgtgg caatttgaaa aagtgttgcc
    13081 atgtaatttc gcattgtcct aacttgactt gtcttttgcc atttgaagac aatttattac
    13141 agcccagatt ttttttctgt ttggccaaat aattgatttt tcctggaggt cttttatgtt
    13201 atatttcgga ttggatatca cctattggtt aataatgata ccattttttc tttttgcctt
    13261 atgggctcaa tccagagtta aagcagcata caaaaagtat tctcgtattg ccatttataa
    13321 cggactcact ggagcccaaa cagcccagca aatacttctt aacaaccgta tcacagacgt
    13381 tcgcgtagag aggggatcag gtcttctttc tgaccactat aacccttcaa aaaaggtgat
    13441 aagattatca cctggagttt atgacagcaa ttcagtcgca gcagtcagca ttgcagcgca
    13501 tgaaaccggt catgccatac aacacgcaaa ggcgtactct cctcttgctt taagaagcgt
    13561 gctggctccg acagcttcta ttggatcaaa ttttgccttc attattattt ttgcgggctt
    13621 gcttattaat atggcaggtt tgataaaatt cggtatcgcc ctgtttgcga ttgttgtatt
    13681 ctttcagctt gttacattgc cggttgaatt tgatgcatct gccagggcaa agagggcact
    13741 ggtttcgtac tcaatggtaa acaataccga actgaaagga gtcaatactg tactcaatgc
    13801 tgccgcaatg acctatgttg ccgccgcagc ttcagcgatc gcgcaacttc tatatcttct
    13861 tctaagatca ggctttcttg gagacgatta aaaccgtaaa attataatct ttagttacaa
    13921 gaaaaggctc ccaaaaggtg ccttttctta tttaaaacta gatcattaac acttatactt
    13981 aatcttctat tttgccaaaa gctcgttagg tgtacctatc tcaatataag aagctcctct
    14041 cgcagctcca aaattaccgg ccacaataac gattctgtct tcacgactta tagcttttct
    14101 ttccagaatg taaccaagcg cattttcaat aaagcccctg tgactgtcct gaacatcaag
    14161 gtaattcgcg tacacaccgt aagaaagagc taattccctt actgttcttt tgctatagca
    14221 ttcagcataa accagggcac tacctcgata aacagagata cttctggccg ttctgccagt
    14281 caatgtatct ataacaatgg ctgttgtatt aagttccgcc gcggcgtcaa cagcagcttt
    14341 tgccagatat gcagttattt cgttaaagac cttctcctgt gtagtggtat tatatctgtc
    14401 aagaccgacc tcaacctcac aagctatttt gctcatcatt tgaaccgatt ccagaggata
    14461 tttcccataa gcggtttctc cacttagcat aagcgcgtca gttcggtcat aaacagcatt
    14521 tgctacatca ctaatttctg cgcgtgtagg acgcggatta tctatcatcg aatgaagcat
    14581 ttgcgtagca acgatgatgg gttttcgtct tgaaagacac tttttgatta ttgttttttg
    14641 tgtatatgga atcttctcgg caggaatctc aattgccaga tccccccttg ctatcattac
    14701 tccatatgcg taatcaataa tctcatcaat tttatctaca ccttcctgat tttctatttt
    14761 agcaattatc tttacactac tgtttttagc gtccaaaata cgctgaatcg ctattacatc
    14821 ttcttttcgt ctgacaaacg agtgcgctat aaaatcgata tcgttttcaa tgcaaaactc
    14881 aatatattcc cgatcacgat cactcaatga tggtaaatca agactgacac caggaacatt
    14941 aatggatttt ttccccttaa ttacaccact attcattaca cgacatttaa ggcgatcccc
    15001 ctccttctct tccacaagca gctcaagatc accgtcatca atcaaaatcc tattaccaac
    15061 tggcacatcc cttacaaacg agctataaga tacattgata cattcagcgc tacacttttc
    15121 atcaggattc cccgctaaat agacaatatc atcagcttga acggaaatac cactatctgc
    15181 ggcagtcgta cggatttccg gtcccttcgt atcgaccaga atcgcaatct tttccgaaac
    15241 cgttctgata ttttttacca cagacaacgt ctcatgtggt gactgatgcg cagtattaag
    15301 acgtaccaca ttcatacccg cattatacaa tgacctgata aaatctacat cacaacgcat
    15361 atcagaaata gtagcaacaa tctttgtttt cttcttgaac atatattgat tacctcaatt
    15421 taattattca tattgttatt aaatataatt tttagcatcg atacgtctca atatatatta
    15481 agaacagtgt cgataccagg cagcagatat ttcacagcca acacttagac caaatgttat
    15541 tgcagtataa taatggcaat ctttttttta aaactaccac agcccctaag a
//
LOCUS       DAOTGZ010000040        15506 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_3064
            NODE_4606_length_15506_cov_4.897806, whole genome shotgun sequence.
ACCESSION   DAOTGZ010000040 DAOTGZ010000000
VERSION     DAOTGZ010000040.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868088
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 15506)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 15506)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:22:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,785
            CDSs (total)                      :: 2,751
            Genes (coding)                    :: 2,732
            CDSs (with protein)               :: 2,732
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 12 of 19
            Pseudo Genes (internal stop)      :: 5 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15506
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4606_length_15506_cov_4.897806"
                     /isolate="AMR_MDS_3064"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..2027
                     /locus_tag="P5123_04345"
     CDS             <1..2027
                     /locus_tag="P5123_04345"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: protein motif:HMM:NF012403.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HRX15522.1"
                     /translation="AVEDDSKGYILYVRDYNIEQTYINLLLTIVEDCYLEQDRFFGGS
                     IKPEKFIINYTDANTFLIEYGARLATEGYEIRFKQKKIRPISAGGRVQKRVTSGIDWF
                     EISLSYKDYEGEEYSLKIEEDLLKSGLIRHKDEYIPLSPEDIQQLKTISQNTIYKNDK
                     YNISKYNFARIEELKETVADKEKKIFEETMALYERLKNFKSIKKYALPKKFKAKLRDY
                     QKEGYRWLMFLKEYKVNGCLADDMGLGKTIQTLALFQKLKEEANFKTSLLVVPVSTIP
                     NWSSEIERFCDNFNVAIHLGQSRIREINFILSHDLIITSYHTLARDIKFFKDIKFYYI
                     VIDEAQAIKNSRSQSFETITSINAKHRISLTGTPVENSCSDLYSQMEFLHPGLLGTQH
                     SFRKNYLYPIEKSNDKEVAEDLRKTVFPFIMRRKKEDVARDLPEKEEITYYVEMHPKQ
                     HKLYETYRKKIKGDLDKEIEKKGLPGASMAILEAMLRLRQMALFPQLVNQYFDPVESA
                     KFEVLKDLLEEITSENHKVLVFSQFVESLKIIRSYCDSMKWDYSYIDGSTKKREIQIK
                     NFQSNEKIKIFLLSIKAGGVGINLTAADYVIIFDPWWNPAVESQAIDRSHRIGQINKV
                     IAYKLIVKNTIEEKMLELQNRKKDLVNNLITEEKSFFKSLTKNDIDYILS"
     gene            2084..3247
                     /locus_tag="P5123_04350"
     CDS             2084..3247
                     /locus_tag="P5123_04350"
                     /inference="COORDINATES: protein motif:HMM:NF012692.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family transcriptional regulator"
                     /protein_id="HRX15523.1"
                     /translation="MADIAKLNIINSSRIIRQIWQNPGISRIEIAEQLKLDRSTITKI
                     MKNLMDKGWILSQSKDSRENVGRKPVGLTLNTQLGLILGIEIKTENCSAIVTDLNGEI
                     IHSELFSFEPDKETIEDISLNIYNSIKNNASINGRKILMVGIGISGIVDPFTGTVLMS
                     NPMKIESPRNLSETIENKIKIPVIIENDANCCCWAQIVYRKESSFKNFLSLMGEFRST
                     DLYGLREPGVAVGIGMVINGEVYYGENFTAGEFRSVDWKPGLITQFSLDNAITNSISN
                     KPEALSLMFKELAKNIALVINICNFNHVMIAGDFVKHKDEILKTLKNAVTENWVYSNL
                     EEKKISIDFLPFDENAIAFGAACHVLSRIFSIPQLGKEKLMRGTILLEHLIGL"
     gene            3325..3681
                     /locus_tag="P5123_04355"
     CDS             3325..3681
                     /locus_tag="P5123_04355"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRX15524.1"
                     /translation="MKKILLLALFVSLFASCATAKINVVESQPTSTITMSNGFTMYNI
                     DGKAPSKQPSGLFSRTYIVPAGTHTISVQQTNSILLKNVKGTITHNFLANKTYKVVKK
                     NSFSVANADAFELVEK"
     gene            complement(3787..9450)
                     /locus_tag="P5123_04360"
     CDS             complement(3787..9450)
                     /locus_tag="P5123_04360"
                     /inference="COORDINATES: protein motif:HMM:NF013225.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxyl transferase domain-containing protein"
                     /protein_id="HRX15525.1"
                     /translation="MSADILKAVNESIDRLKELNPEINGDTIIRTNRPHRLKKVVVAN
                     RGEIAKRFFLSLHEEEIPSVAVVTEVDRGQSWYEFADEVVFIGEETNYTNIPVIIGAA
                     ALVEANAIYAGYGFLSENASFVENIDEYAKLSSKDIFFMGPTSQTMRLMGDKVSSREL
                     AKKNGVPLFESTECVDPTDIEGIKKEIERVGYPVIVKLSAGGGGKGMYPVYKEEELKD
                     AVDSCVRIGKDLYNDSRFFIEQYIQEPVHIEVQVFNDRALGIRKCAVQRRNQKIIEES
                     GHTFLPDHLAFSFLSAAERLAIESGYGNCGAGTVEFLIDAATGKVGFMEMNTRLQVEY
                     GVTDQSLGIDLAKWQILYFDGRGDEIEDTAAIKYRYFARMHSIECRIYAEEPENDYRP
                     SPGTITEINLPAFNGIRCDFGFGEGDKILSMYDPMIGKLIAYASSREEALIRMERALQ
                     ELYIKGVKTNINQLLKIVRHDSFKDKDYTNLLIPKNDELGFLGPDHKYLSAVSSSRQI
                     IFGAFGEYVKYQYDNAQKFSVVAASKGILDTDAADFPYRFNVEFEGEAHTVEFMQENI
                     DTVHVLLDNQYRGKLSLHEVNDRLDDLAIGFDSRIRRVRVERHIGFCIVRMKDDSNKY
                     NYYRMNIVPEGVEEADTSGHVCSPFQGTFVSFCGTHKVGDQVKEGDKLVILSAMKMET
                     IITAPVDGTISYIIEDGDVSRLQIGTTSDGRVIGKAVQEGEVIFRIDGDKKEEESGKT
                     AAVINASDTQNTYELLLAEGGCDKLLENIEDHFDNLMLLFTAMIKGFLRQAAVIERLK
                     EIMNKLPKEQWKALMTREHSDQICKLILHYTYVRRLYSLVVSEEGFSFPDELDNFIRG
                     WNSAEIEFSDGFYRMLMDTFNSYGIRRWKRGAGNSRSNIFRFFYFLNLSYESILRNWS
                     RVGKQTNIVGNLDPTSPLTQQTLKKLVEHTGHQIDDSSERFLKRVIDNYFPEREAELY
                     AGVVDGSDNKTACSTCKDISSLDGVKKVDGGKLLYSIVSSGDLANAKYSYEKDHGLLI
                     CGNDETCAALYVAEYPGDKEIVSCTSNVKTALAVFAQLKNDQKSACRFELVLDKALFS
                     LEAGEHNASGYRQLVQIAKPLFKSILAGDITYGLIHADLTCDGNSKASVFKVASVNNR
                     MVLDLLFDNDPLNPYKKVEPSAADSKMYALDKWPIENWAAVMFDGGNYEEVIVELADR
                     GEKPAGSKIFTGKVGGSEACFYMKDYRVIGGATGNREGLKYSAACYLAYMKGWPLYVW
                     NDSAGANIKEGMVSLNRGGQGFMMNSLLAANVPLEKFKNYVTNIYDEDVKAVFKDMDI
                     KYGLSLEQDRGDKKTMVVAIGTGASAGLDVYGSSQATVQIILDSENSYRVLTGSNVIK
                     SVMGEDISNYDIGGATILGKWTGIVDLVACDKPDMIRKLNEIQYIFCSKTKSEVIKRN
                     SEKLVSFTEKYQGAVIDEALVRNNVDEGKFFIYKEDYYASGALLAGFALLGGTRTLIM
                     GARTNSGLRSKSCVTKGRELLRMAYRTSTPQILIYGDSWIRRSTSGTHLRAYYDFMNE
                     LTNRSSHRIHIVTSAAALHNFEVTSCADAVIFVKNESLSELDEKFASQNAAFVVNSME
                     EAFDVSANIFALLEKRSTEEVKSGKDKINVPDDKGTPFDIIDSVITPAFDDESFVEFY
                     APFNQPSGPNLVTGLARIKGRAVGIVADQPKIKGGGADALGTEKFRVFVQFLNRKNIP
                     LVMLSNSSGFVPGSQQERYRIQAIGADSLDENVLGEIPVVSVVLNQNYGGRQIQSFSK
                     SLRPGIYYTARHDATLAVMGATAAYDLLGAKNYSKLVAEGKQSEADAYQKKFMDDYLT
                     KAMAENDATATKVMDSVFKDVSTLRDHIASSLVKAEDECFKAFGK"
     gene            complement(9523..15489)
                     /locus_tag="P5123_04365"
     CDS             complement(9523..15489)
                     /locus_tag="P5123_04365"
                     /inference="COORDINATES: protein motif:HMM:NF026115.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HRX15526.1"
                     /translation="MFNSAAEGLAKELASVKIDVTKAVRIVSNVTAKPYPADESAIKD
                     LLKKQMVSPVEFVESIANIKKAGVNTLIEAGPGRLLCNLVSKMETSFNDVIASVDPKK
                     GEAASTAAALEKVKALFGAKTVSTAAVPAVAAPIAPSASSYTFKADDAGFESFLKNND
                     AQLKELLYQEYIKARKEEAFNAVEKRGIYTGKVVIPGVSIGLPGLSNKVFSENNFDKL
                     LKGTNLIDAVSEENQNLVIDKNITRILKEANGNARYVEISSTDDVMHLAGQLGYFDLT
                     TEYGFKYDYDSAYALAIAAGIEALKDAHIPLILSHKTTSTGSMLPDRIMLPKDMQEST
                     GVIFTSIFPGFESLLEEVSGYYYEKFVRAPYKEFESLYYFLMEKVGDTAVKDKLTEWF
                     FNRKKELKEGHYSFDRNFLIRILALGSAHFAQFIGAKGPNTHIDAACASTTQAVAMAE
                     DWIRMGRCERVIVIGGENATSKNMNQWIGSGFLALGAATVKKSVEEGAKPFDETRNGM
                     IVGSGAVGIVVERSDKVEARGLRGQAEILGTHIGNSAFHVSKIDVNHLTEEMVAFFDK
                     IDNLHGLNRAEYASKMVFMSHETYTPARGGSADAEVSALTHTFGSNVNSITVTNTKGY
                     TGHTLGAGIEDAVMVKALVKGTVPPVANLTKIPENFKSLKFSKGEQGDFEYGFHLGAG
                     FGSHFAFLFIRKEKENSVVNNPTYTKWLQSVTGKENPVTEIKDNILVADSVPGIFNGK
                     ANPVAASAPVSAPVVSAPVAVSAPVATPAADTSSVIAKIKTIIADQTGYDTDMLENDL
                     DLEADLGIDTVKQVEIFGKISDSYNLAVPEDLKLSDLNTIQKLGEYIAAQTGGSSVAP
                     ASTAGSAGADASEVMGRIKAIIAEQTGYDIEMLEEDLDLEADLGIDTVKQVEIFGKIS
                     ESYSLAVPEDLQLSDLNTIKKLSGYIASKAGGSLASTVAAPAQSGGADKGAVTAKIKG
                     IIAEQTGYDTEMLEDNLDLEADLGIDTVKQVEIFGKISESYSLAVPEDLQLSDLNTID
                     KLAGYIAERAGGSSAAPAVAAPAATAGGVDKGGVTAKIKGIIAEQTGYETDMLDDNLD
                     LEADLGIDTVKQVEIFGKISESYSLAVPEDLQLSDLNTIDKLAGYIAERAGSAAAPAK
                     VAPAPAAQAPVKSAVSETVSKENGIYRFSMTVEERPLNAKKSDVFKGKRYIVIQDSHG
                     FADEFIEFAKNAGSTIVTAGTGEGCDYSLDVKTYDSLQKSADAIADAVKDINGIIHFG
                     AVDGYYAKKLSDSDINLAVKSQFILAQKYFDDLNRDGSLIAALSFNSVVFAYGKTENK
                     MEPLFAGVSGLYKTLNKEIAPARVKVVDFDVKDPKKSSKSVMENFITEIASDNKEVEA
                     GYKGSKRYGIRLVDEVPVKGDSIVKNGDRFLVTGGARGITFNILMSVVKEYKTDLVIL
                     GRSDVSKVDPKYLDASVTEKTIFDDLKTSMTGSKPLEIKKAASKIMTLVETVRNIEEL
                     KNQGVQVDYHACDVTDSKRVKQIVSSYDRIDGVLHAAGVEISQFLTKMDAWSFNMVFD
                     VKVNGLRNLIEALKDMDYRYMMTFSSVTARFGNEGQTSYTSANDMIGKMLQRQKQLNP
                     DRAYKVYDWTAWEGAGMATKETVKKVLEARGLEFVPLDFGVQCFMDDLCFADRTEVLV
                     SGMDHAFDPDKILPQPGMGGVDYFLDREVESKDGYKKFERLLTAERDLFLFDHEKDGV
                     PLFLGATGLETMAEASSRVGDGELIGMAKYQIPYGIKLLKNRPKALDITAEKRGASVL
                     AQITSVFKNPAGKVMGDPKLHYQAEFIFGKPLADKKITVPEFKPALITGSFQDLIYHP
                     KRLFMHGLFKTVEELLSFEEERMITRVCNSSDREFFAGVQNPEFTTDPVLVDAMFQTG
                     GMLDVMTTEKIILPYMMKEMKFYRKPEKNVPYLCITEKTAATADINTYNLTLCSEDGQ
                     VYLEILDFEMIQVGDVEPGMSIKDKIKLQK"
BASE COUNT         4605 a         3572 c         2835 g         4494 t
ORIGIN      
        1 aagcagttga agatgactca aagggctata tcctttatgt gcgtgactac aatattgaac
       61 agacatatat taatcttttg ttaacgattg tagaagactg ttatttagaa caagatcgat
      121 tttttggagg gtccataaag ccggaaaagt tcattattaa ctatacagat gctaatactt
      181 ttttaatcga atacggagca cgtcttgcaa ctgaaggtta tgaaatacgt tttaaacaga
      241 aaaaaattcg acccatctct gctggaggga gggtacagaa aagagttacc agcggaattg
      301 actggtttga aatcagcctt agctataagg attatgaagg ggaagagtac tctctgaaaa
      361 ttgaagaaga tcttttaaaa tccggcctta tcagacataa agacgaatat attccgttaa
      421 gccccgaaga catccagcaa ctaaagacaa ttagccaaaa tacgatttat aaaaatgaca
      481 aatataacat atctaagtat aatttcgctc gtatcgaaga gttaaaagag accgtagcgg
      541 acaaagaaaa aaagatattt gaagaaacca tggctcttta tgaacgtctt aaaaatttca
      601 aatcgattaa aaaatatgcc ttaccaaaaa aattcaaggc aaaacttcgt gattaccaaa
      661 aggaaggcta tcgttggtta atgtttttaa aagaatataa ggttaacggc tgtctcgcag
      721 acgacatggg actgggaaaa actattcaga cactggcact ttttcaaaaa cttaaagaag
      781 aggctaattt taaaacttct ctacttgttg ttccggtaag cacaatacct aattggagtt
      841 cggaaatcga gcgtttttgc gataatttta atgttgcgat acacctaggt caaagccgaa
      901 ttcgagaaat aaattttata ttatcacatg atctgatcat tacaagctac cataccctcg
      961 cccgtgatat taaattcttt aaagatatta aattttatta tatcgttatc gacgaagccc
     1021 aagcaatcaa gaattcccga tctcagtcat ttgaaaccat cacatccatc aatgccaagc
     1081 atcgtatttc actcacaggc acaccggttg aaaactcttg ctccgatctt tattctcaga
     1141 tggaatttct tcacccaggc cttcttggca cccagcactc ttttagaaaa aactatcttt
     1201 atccaataga aaaaagcaac gacaaagagg tggcggaaga tctgcgaaaa acagtttttc
     1261 cctttataat gcgacgcaaa aaagaggatg tggctagaga tttacccgag aaagaagaga
     1321 taacctatta tgtagagatg caccccaaac agcataaact ttatgaaaca taccgtaaaa
     1381 aaatcaaagg tgatcttgac aaagagatag aaaagaaagg tctacccgga gcctcaatgg
     1441 caattcttga agcaatgttg cgcctacggc agatggcact attcccacag ctggttaatc
     1501 aatattttga tccggttgaa tctgcaaaat ttgaagtatt aaaagatctt ttagaagaga
     1561 taacatccga aaatcataaa gttcttgtat tttctcaatt tgtagaatca ctaaaaataa
     1621 ttaggtcata ttgtgactct atgaaatggg actactcata tattgacgga tccacaaaaa
     1681 aacgtgagat ccaaataaaa aactttcagt ccaacgaaaa gattaaaatt tttctattaa
     1741 gtattaaggc cggtggagta gggattaatc tgactgcagc cgattatgta atcatctttg
     1801 atccatggtg gaacccggct gttgaaagcc aggcgattga ccgtagccat agaattggtc
     1861 aaatcaataa agtcattgcc tacaagctaa ttgttaaaaa cacaattgaa gagaagatgc
     1921 tagaacttca gaatcgcaaa aaagatctgg taaacaatct tattacagaa gaaaaatcct
     1981 tttttaagtc actaacaaaa aatgatatag attatatttt gagttaaatt ttgcaaaact
     2041 taatgaatat tgactttttt ttgtgggtat taataattga cgcatggcag atatagcaaa
     2101 actgaatatt ataaactcct cacgaattat ccggcaaatt tggcaaaatc cgggtataag
     2161 tcgtatcgaa attgccgaac agctaaaact agaccgttca accatcacaa aaatcatgaa
     2221 aaaccttatg gacaagggtt ggatactgtc gcagagcaag gacagccgtg aaaatgtagg
     2281 cagaaagcca gttggactga cactcaacac tcagcttgga ctaattttag gaatagaaat
     2341 aaaaacggaa aactgctcgg ctattgtaac cgatcttaat ggcgaaatta tccattccga
     2401 gttattcagt tttgagcccg acaaagagac catcgaagac ataagtttaa atatttataa
     2461 ctccataaaa aacaatgcct ctataaatgg tcgaaaaatt cttatggtcg gcattggtat
     2521 ttccggaatc gtagacccct tcacgggaac agttctaatg tctaacccca tgaaaattga
     2581 atcaccgcgc aatttgtctg aaaccattga aaacaagata aagattccgg ttataattga
     2641 aaacgacgct aactgctgct gctgggctca aattgtttac cgcaaagaga gctcgtttaa
     2701 aaatttttta tcattgatgg gcgaattcag aagcactgat ctttatggct tacgtgagcc
     2761 cggggttgct gttggtatcg gaatggttat taacggagag gtctattacg gtgaaaactt
     2821 tactgccggt gaatttcgca gtgttgactg gaaaccggga ctcattaccc aattctcact
     2881 cgacaacgcc ataaccaatt cgatcagtaa taaacccgag gcactatcgc tgatgtttaa
     2941 agagctagca aagaatattg cacttgttat taacatctgt aattttaacc atgttatgat
     3001 cgccggcgac ttcgttaaac acaaagatga aattttaaaa acattaaaaa atgccgttac
     3061 cgaaaattgg gtatacagca atctggaaga aaaaaagata tccattgatt ttttgccttt
     3121 cgacgaaaat gcgattgcct ttggtgccgc atgtcatgtt ctctccagaa tcttttccat
     3181 tccccagctt ggtaaagaaa aattgatgcg cggcactatt cttttagaac atttaattgg
     3241 tttataattt aacatttata attgactaaa ataatgattt taaccattat agaccaaaat
     3301 tgctaaatta ccgaggagaa aaacatgaaa aaaatacttt tgctcgcact tttcgtttcg
     3361 ttatttgcgt cttgcgcaac agccaagatt aatgttgtag aaagtcagcc aacttcgacc
     3421 ataacaatgt caaatggttt tacaatgtat aatattgatg gcaaggcgcc ttccaaacag
     3481 ccatccggtc tattttcaag aacctatatc gttccagcag gtactcacac aattagtgtt
     3541 cagcaaacta actcaattct gttaaaaaac gtcaagggaa caattactca taactttttg
     3601 gctaacaaga cgtataaagt cgttaaaaag aactctttta gtgtcgcaaa cgcagatgct
     3661 tttgaactag ttgaaaaata gattcatttg agtttctgca tttccaaaat tttaatatga
     3721 atccaaatat tcttaatata aaagccgggc ttaagcccgg ctttattatt ttcacaaaac
     3781 cctatattat tttccaaaag ccttaaaaca ctcatcttca gcttttacta aagatgaggc
     3841 aatatggtct ctcaatgtgg acacatcttt aaagaccgaa tccatcactt ttgtcgcagt
     3901 cgcgtcgttt tcggccatag ccttggttaa ataatcatcc ataaacttct tttggtaagc
     3961 atcggcttcg gactgtttgc cctcggcgac cagtttactg taattttttg caccaagaag
     4021 gtcataagcc gctgttgctc ccattacagc aagagtcgca tcatgacgcg ctgtatagta
     4081 aatacctggc ctcaaagact tgctaaacga ctgtatctga cgaccaccat agttctggtt
     4141 cagaacaacc gaaactaccg gaatctctcc aagtacattt tcatccagcg agtcagcacc
     4201 aatcgcctgt atcctataac gttcttgctg ggatcccgga acaaaaccag aagagttcga
     4261 aagcattaca agaggaatgt tctttctgtt taaaaactga acaaaaaccc tgaatttttc
     4321 ggtacctagg gcatcagcgc ccccaccctt gatctttggc tgatcggcta ctattcccac
     4381 agcccgtccc ttaatacgag caaggccagt aaccagattc ggaccgctcg gttgattaaa
     4441 cggagcataa aactctacaa agctctcatc atcaaaagcc ggcgtaatta ccgaatctat
     4501 aatgtcaaaa ggagttccct tatcatcagg cacatttatc ttgtctttgc cactcttcac
     4561 ttcctctgta cttctttttt caagaagggc aaaaatattg gccgaaacgt caaaagcttc
     4621 ttccatactg tttacaacaa aagcagcgtt ttgtgatgca aatttttcat ccagttcact
     4681 taacgattcg tttttaacaa agattaccgc gtccgcacat gaagtcacct cgaaattatg
     4741 aagtgccgca gccgaagtaa cgatatgtat gcgatgcgag cttctgtttg taagctcgtt
     4801 cataaaatcg taatatgcac gcaaatgagt tcccgatgtc gagcgtcgta tccaactgtc
     4861 tccatatatg agtatctgtg gagtcgatgt ccggtatgcc attcttaaaa gttcacgccc
     4921 tttggtcaca catgacttgg atcgtaatcc actgtttgta cgtgcgccca ttatcagtgt
     4981 tcgtgttcca ccaagcaggg caaatcctgc gagaagagca cccgaagcgt aataatcttc
     5041 tttataaatg aaaaacttgc cctcatcaac attgttacga acaagagcct catcaataac
     5101 tgctccctgg tatttttcag taaacgatac caatttctcg ctatttctct taataacttc
     5161 agattttgtt ttggaacaga agatatattg tatttcgtta agcttacgaa tcatgtccgg
     5221 cttgtcacaa gcaaccagat caactattcc agtccatttg cccagaatgg ttgctccgcc
     5281 aatatcataa ttagagatat cctctcccat tacagatttt attacgttag atcccgttaa
     5341 aaccctgtaa gaattttcag aatcaagaat tatctgtacg gtagcttggc tagaaccgta
     5401 aacgtcaagt ccagcagagg cgcctgtacc aatagccact accatcgttt ttttatctcc
     5461 cctgtcctgt tccagcgaaa gaccgtattt aatatccata tctttgaaga cagccttaac
     5521 atcttcatca tagatatttg taacatagtt tttaaacttt tcaaggggaa cattagccgc
     5581 caagagggag ttcatcataa agccctgtcc gccacgattt aacgagacca tgccttcctt
     5641 gatattcgca ccagccgaat cgttccacac gtaaagaggc caacccttca tataggcaag
     5701 ataacaggca gccgaatatt taagtccttc cctgtttcct gtagcaccac ctattacacg
     5761 atagtctttc atataaaagc aggcttcact acctcctact tttcctgtga atatctttga
     5821 tccggcaggt ttttctcctc tatccgccaa ctcaacaatc acctcttcat agttgccacc
     5881 atcaaacatc acagcggccc aattttcgat aggccactta tcaagggcat acatctttga
     5941 atcggccgca gaaggttcta cttttttata cggatttaaa ggatcattgt cgaaaagcag
     6001 atcaagtacc atacggttat taacagaagc aaccttgaat actgaagctt tactgtttcc
     6061 gtcacatgtc aaatcggcat gtataaggcc atatgttata tcaccagcca atattgattt
     6121 aaaaagaggc ttggctatct gtacaagctg tctatacccc gaagcattat gctcacccgc
     6181 ttcaagagaa aagagagcct tatccagaac aagttcaaaa cgacaggcac ttttttggtc
     6241 attttttagc tgagcaaaaa cagcaagtgc agttttgaca ttgcttgtac atgacacaat
     6301 ctctttgtct cccggatatt ccgcaacata aagagctgca catgtttcgt cgttgccaca
     6361 tatcaatagt ccatggtctt tttcataact gtatttggca ttggcaagat cgccagacga
     6421 gacaatagaa tagagcagtt ttcccccatc aacttttttt actccgtcaa gagaggatat
     6481 atctttacat gtagagcaag ccgttttatt atcagaacca tcaaccactc ctgcgtaaag
     6541 ttcggcttca cgttcgggaa aatagttatc aataactcgc ttcagaaatc gctccgatga
     6601 atcatcgatt tgatgcccag tatgctctac aagtttctta agagtctgct gcgttagcgg
     6661 agatgtaggg tcaagattcc ctacaatatt tgtctgtttg ccaacacgac tccaatttcg
     6721 caagattgac tcatacgaca agtttaggaa atagaagaac ctgaagatat tggatctgct
     6781 gtttccggct ccacgcttcc accgacgaat accgtaagag ttaaaagtgt ccattagcat
     6841 gcggtaaaag ccgtcagaaa actctatctc ggcactattc catccgcgaa taaagttatc
     6901 aagctcgtca ggaaaagaga agccctcttc agatacaaca agcgaatata gacgacgaac
     6961 gtatgtgtaa tgaagaatca atttacatat ctgatcactg tgttcccttg tcataagagc
     7021 cttccactgt tctttgggaa gcttgttcat tatctctttt agtctttcaa taactgcagc
     7081 ctgacgaaga aaacctttta tcatagcagt aaaaagaagc attagattgt caaaatgatc
     7141 ttctatattt tcaagaagct tgtcacaacc gccttcggca agaagaagct cataggtgtt
     7201 ttgagtgtcg gaagcgttaa taactgccgc tgtttttccg ctctcctctt cttttttatc
     7261 accatcgata cgaaatataa cctctccctc ctgaacagcc ttgcctatca cgcgtccatc
     7321 agaggttgta cctatctgaa gccgggatac atcaccgtct tcaattatat aagatatggt
     7381 tccatcaacg ggagcagtaa ttattgtctc catcttcata gcggataaaa taacaagttt
     7441 atcgccctct ttaacttgat caccaacctt atgagttcca caaaaagaga caaatgttcc
     7501 ttgaaaaggc gaacagacat ggcctgaagt gtcagcctct tcaacacctt ctggaacaat
     7561 gttcatacgg taataattat atttattact gtcatctttc atacgcacaa tacaaaagcc
     7621 tatatgacgt tcaacacgca ccctgcgtat gcgcgaatca aagccgatcg caaggtcatc
     7681 tagacggtca ttaacctcat gaagtgaaag ttttccacga tattgattat caagcagtac
     7741 atgaactgta tcgatattct cctgcataaa ttcaacagta tgtgcttcgc cttcaaactc
     7801 aacattaaag cggtatggga aatcggcagc atctgtatcc agtatgccct tagaagcggc
     7861 aacaacactg aatttttgtg cgttatcata ctgatactta acgtattcac cgaaggcacc
     7921 aaaaataatc tgcctgctgc ttgatacggc cgatagatac ttgtgatcgg gccctaagaa
     7981 gccaagttca tcgttttttg gtattagcag attcgtgtaa tctttatctt taaaactatc
     8041 gtgacgtaca atttttaaaa gctgatttat atttgtcttt acacctttta tgtagagttc
     8101 ctgaagagct ctctccattc ggatcaatgc ctcttcacgg gaagacgcgt acgcgattag
     8161 ttttccgatc atcgggtcat acatcgacaa aatcttgtca ccctcaccaa aaccgaaatc
     8221 acagcgtatt ccgttaaatg ccggcagatt tatctccgtg attgttccag gagacgggcg
     8281 ataatcattt tccggctctt cagcatatat acgacactct atagagtgca tacgtgcaaa
     8341 ataacgatac tttatggcag cagtatcttc tatctcatca ccgcgtccgt caaaatagag
     8401 gatctgccac ttcgcaaggt caataccaag cgactgatcc gttacaccgt actctacctg
     8461 aagtctggta ttcatctcca taaagccaac tttaccagtc gctgcatcaa tcaaaaactc
     8521 gacggttcct gctccacagt taccgtatcc tgactctata gcaagtctct cagcagcaga
     8581 cagaaacgaa aacgcgaggt ggtctggtaa gaaagtatgt cccgactctt caatgatttt
     8641 ctgattgcgc ctctgtacgg cacattttcg aataccaagt gctctatcat taaaaacctg
     8701 tacctctata tgcacaggct cctgtatata ctgttctata aaaaagcgtg aatcgttata
     8761 caaatccttt ccaatacgca cgcacgaatc cactgcgtct ttcaactctt cttctttgta
     8821 aacagggtac atcccctttc cgccgccacc ggccgagagt ttaacaataa cggggtatcc
     8881 cacacgttcg atctcttttt taataccttc aatatctgtt ggatcaacac attcggtact
     8941 ctcaaaaagc ggaactccgt tcttctttgc caattcacgc gaacttacct tgtcacccat
     9001 aaggcgcatg gtctgcgatg ttggccccat aaagaagatg tcttttgaag agagcttagc
     9061 gtattcatca atattttcga caaaagaggc attttcagaa agaaagccat aaccggcata
     9121 tattgcgtta gcctcaacca atgctgcggc tccaattata accggaatat tggtgtagtt
     9181 ggtctcttct ccgataaaca ctacctcatc cgcaaactca taccaacttt ggccgcggtc
     9241 aacctctgtt acaaccgcaa cagatgggat ctcttcttca tgaagcgata aaaaaaagcg
     9301 tttcgcgatc tccccgcggt ttgctaccac aaccttcttt aacctgtgag gacggttggt
     9361 tctgataatt gtatcgccgt tgatttcagg attaagctcc ttcaaacgat caattgactc
     9421 attaaccgcc ttaagaatat cagcgctcat acacttctcc atgttgtatt gtttttttta
     9481 ccgggtcttc tcagacccgg tattatatct acgacttgta ttttacttct gaagtttaat
     9541 cttatccttg atactcatac ccggctcaac atcacctacc tgaatcatct caaaatcaag
     9601 aatttcaaga taaacctgtc catcttctga acatagtgtt aaattataag tattgatgtc
     9661 tgccgttgcc gcggtctttt ctgttataca aagatacggt acatttttct caggcttacg
     9721 gtaaaacttc atctctttca tcatgtacgg caaaatgatc ttttctgtgg tcattacatc
     9781 cagcattccg cctgtctgaa acatagcgtc aaccagaacc ggatcagttg taaattccgg
     9841 attctgaact cccgcaaaaa actcacggtc actgctgtta caaactcttg tgatcattct
     9901 ctcttcttcg aaagaaagga gttcttcaac tgtcttgaaa agaccgtgca taaaaagacg
     9961 ctttggatga tagataagat cctgaaagct tcctgtaatc aatgctggct taaattcggg
    10021 aactgttatc tttttatccg caagcggttt gccaaagata aactcggcct gataatgcag
    10081 ttttggatcc cccataacct tgccagccgg attcttgaat accgaagtaa tttgagccaa
    10141 taccgaagcc cctctctttt ctgcagtaat atccagcgcc tttggacggt tctttaacag
    10201 tttgataccg taaggaatct gatatttggc catacctata agttcaccgt caccgactcg
    10261 gcttgaggct tccgccatag tctctagccc tgttgcccca agaaatagtg gaactccatc
    10321 cttctcatga tcaaatagaa acaggtcacg ttcggcagtc aaaagtcgct cgaacttttt
    10381 ataaccatcc tttgattcaa cttcacgatc cagaaaataa tccacaccgc ccataccagg
    10441 ctgaggaaga atcttgtccg ggtcaaacgc atgatccatt cccgaaacaa gaacctctgt
    10501 tctatcagca aaacaaagat cgtccataaa acattgcacg ccaaaatcaa gtggcacaaa
    10561 ctccagtcct cgtgcttcaa gaaccttttt aacagtctct ttggttgcca taccggcacc
    10621 ttcccaagcg gtccagtcat aaactttgta agcacgatca ggattaagct gcttttgacg
    10681 ctgaagcatt ttgcctatca tatcgttagc cgacgtatag cttgtctggc cctcattacc
    10741 aaatcgggct gttacagaag agaatgtcat catatatctg taatccatat ccttaagggc
    10801 ttctatcaaa ttgcgcagac cgttaacctt gacatcaaag accatgttaa acgaccaagc
    10861 atccatcttg gttaaaaact gacttatctc tacacccgca gcatgaagca ctccatcaat
    10921 tctatcatac gaagagacta tttgtttaac tcttttacta tcagtaacat cacaggcgtg
    10981 ataatcgacc tgaacgccct gatttttaag ctcttcgatg tttcgcactg tttcgaccag
    11041 agtcatgatt ttagaggctg ctttcttaat ttcaagaggt ttgcttcctg tcatgcttgt
    11101 ctttagatca tcaaagattg tcttctctgt aaccgatgca tccagatact ttggatcaac
    11161 cttcgaaaca tcacttcgac caagaataac cagatccgtc ttgtactcct taacaaccga
    11221 catcaatatg ttaaatgtaa ttccgcgagc tccccctgta accagaaagc gatctccgtt
    11281 tttaactatt gaatcaccct taaccggaac ttcatcaaca agtcgtattc catagcgctt
    11341 gctgccttta taacctgcct cgacctcttt gttatcagaa gcaatttcgg taataaaatt
    11401 ttccataaca cttttactac tctttttggg atccttaaca tcgaagtcca caaccttgac
    11461 acgagctggc gcgatctctt tgttaagagt cttgtaaaga cctgagactc cggcaaaaag
    11521 cggctccatt ttgttctcgg ttttaccgta ggcaaataca accgagttaa atgaaagtgc
    11581 cgcaattaaa gaaccgtccc tgtttaagtc atcaaaatac ttttgagcca gaataaactg
    11641 cgatttaacc gcaagattta tatcagaatc gctcaatttc ttggcgtaat atccatcaac
    11701 agcaccaaaa tgaattatgc cgttaatatc ctttaccgcg tcagctatgg cgtctgccga
    11761 tttttgtaaa gaatcgtagg ttttaacatc aaggctgtaa tcgcaaccct ctccggtacc
    11821 ggcagtcact attgtgcttc cggcattttt cgcgaattct ataaactcat cagcaaatcc
    11881 atgcgaatcc tgtattacga tatatcgttt acccttaaaa acatcactct ttttagcgtt
    11941 tagtggacgc tcctcgacag tcatcgagaa acgataaatt ccgttctctt tcgatactgt
    12001 ctctgaaaca gcacttttta ctggagcctg agctgctggt gctggtgcca ccttagctgg
    12061 agccgcggca gatcccgcgc gttcagcgat atagccggct aatttatcta tcgtgttaag
    12121 atctgaaagc tgaagatcct ctggtactgc aagagaatag ctctctgata ttttaccgaa
    12181 tatctctacc tgtttaacgg tatctattcc gaggtcagcc tcaagatcaa gattatcatc
    12241 aagcatatcg gtctcgtaac ctgtctgctc ggcaatgatt ccctttatct tggctgtaac
    12301 accacccttg tcgactcctc ctgctgttgc cgccggagcc gcaactgctg gtgcagctga
    12361 agatccaccc gcacgctcag caatataacc ggcgagttta tcgattgtat taagatctga
    12421 aagctgaaga tcctctggca ccgcaagtga atagctctct gatattttac cgaatatttc
    12481 tacctgttta acggtatcta ttccgagatc ggcctcaaga tcaagattat cctccagcat
    12541 ctctgtatca taacctgtct gctcggcaat gattcccttt attttagctg taaccgcacc
    12601 cttgtctgct ccacccgact gcgccggtgc tgcaactgta gaagccaacg agcctcctgc
    12661 tttagaagcg atataaccgg aaagcttctt tatcgtatta aggtctgaaa gctgaagatc
    12721 ttccggcacc gcaagcgaat agctctctga tattttaccg aagatctcta cctgtttaac
    12781 agtatcaatt ccaaggtcag cttcaagatc aagatcctct tcaagcatct cgatatcgta
    12841 acctgtctgc tcggctatta tcgctttgat gcgtcccata acttcacttg catctgctcc
    12901 tgcagaacca gctgtagatg ccggtgccac tgaagagcca ccggtctgtg cagcgatata
    12961 ctctcccagc ttttgtatgg tattaagatc cgatagttta agatcctccg gtactgccag
    13021 attataacta tcagaaatct taccaaatat ctcgacctgt ttaaccgtat cgataccaag
    13081 atcggcttca agatcaagat cgttctcgag catatcggta tcgtaaccgg tctgatcggc
    13141 aattattgtc ttgattttgg caatgacaga ggaggtatcc gcagctggtg tggcaaccgg
    13201 tgccgaaacg gcaactggtg ccgaaacaac cggagcagat accggtgcag aggcagcgac
    13261 cggattagct ttgccgttga atattcccgg cacactgtcc gcaacaagga tgttatcctt
    13321 tatttcagta accggattct ctttacctgt cacagactgt aaccattttg tataggtcgg
    13381 attgttaact acactattct ctttttcttt tcttataaac agaaaagcaa aatgcgaacc
    13441 aaacccggca ccaagatgga aaccgtattc gaaatcaccc tgttctccct tagaaaattt
    13501 gagagatttg aagttttcag gtatttttgt aaggtttgct actggcggaa ccgttccttt
    13561 aacaagtgcc ttgaccatta cagcatcttc aataccagca ccgagcgtat gccctgtgta
    13621 accttttgta tttgtaactg taatgctgtt tacattagaa ccaaaggtat gagttaaagc
    13681 cgaaacttcc gcgtcagctg atcccccgcg agccggagta taagtctcgt gagacataaa
    13741 taccatcttc gaagcatatt cggctctatt taatccatga agattatcta tcttatcaaa
    13801 gaaagcgacc atctcttctg taagatggtt aacatctatc ttgctcacat gaaaagcgga
    13861 attgccaata tgtgttccaa gaatttcagc ctgaccgcgt aagccgcgag cctcgacctt
    13921 gtctgagcgc tctacaacga taccgacagc gccgctacca acaatcattc cgtttcgggt
    13981 ctcgtcaaaa ggtttggcac cctcttctac actcttcttg actgtggctg ctccaagagc
    14041 caaaaatcct gaaccaatcc actggttcat gttcttactt gtagcgtttt caccgccgat
    14101 aactataact cgctcacaac gtcccatacg aatccagtct tctgccattg cgaccgcctg
    14161 tgttgttgag gcacaagcag catcgatatg agtattcgga ccttttgcac caataaactg
    14221 ggcaaaatga gcagaaccca aagcgagtat tcttatcaag aagttgcgat caaaggagta
    14281 gtgcccctct ttaagctctt ttttgcggtt aaagaaccac tcggtcagct tgtctttaac
    14341 agcagtatca ccgacttttt ccattagaaa atagtagagt gactcaaact ctttataagg
    14401 tgctcttaca aacttctcat agtaatatcc ggaaacctct tcgagcaagg attcgaatcc
    14461 agggaagatg gatgtaaaga ttacacctgt actctcttgc atatcttttg gaagcattat
    14521 acgatcaggc aacatcgatc cggttgaagt tgttttgtga cttaatatta acggaatatg
    14581 cgcgtcttta agagcctcga taccagctgc tatcgcaagc gcgtaagccg aatcgtaatc
    14641 gtatttaaaa ccatattcag ttgtcaggtc aaaatagcca agctgaccgg caagatgcat
    14701 aacatcgtcg gttgagctta tctcgacata acgggcgttt ccgtttgctt cttttaaaat
    14761 gcgtgtaata tttttgtcta ttacaagatt ctgattctct tcagaaacag catcgataag
    14821 gtttgttcct tttaaaagct tgtcgaagtt gttctcagag aaaaccttgt tcgaaagccc
    14881 tggaaggcct atagagacac ccggaattac aacctttcct gtgtatattc cccgtttttc
    14941 tactgcattg aaggcctctt ccttacgggc tttgatatac tcctgataca acagctcctt
    15001 aagctgagca tcattatttt taagaaaact ctcaaatccc gcatcatcgg ccttgaatgt
    15061 ataagacgaa gcagacggag caatcggcgc ggctacagca ggtacagctg ctgtcgaaac
    15121 tgtttttgct ccgaagagag cttttacctt ttcaagtgca gcagcagtcg aagcagcctc
    15181 acccttttta gggtcaacag aagcaattac atcattgaat gatgtctcca tcttgctgac
    15241 aagattacat agcagacgtc ccggaccagc ttcaatcagg gtatttactc cagctttttt
    15301 aatattcgcg atagattcga caaattcgac cggagaaacc atctgttttt ttagaagatc
    15361 tttaattgca gattcatcag ccggataagg tttagcagtg acatttgata caatacgtac
    15421 cgctttagta acatctatct tgaccgaagc gagttctttt gccaggcctt cggcagcact
    15481 gttaaacatt ggagtatgaa aagccc
//
LOCUS       DAOTGZ010000041        15243 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_3064
            NODE_4696_length_15243_cov_4.643864, whole genome shotgun sequence.
ACCESSION   DAOTGZ010000041 DAOTGZ010000000
VERSION     DAOTGZ010000041.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868088
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 15243)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 15243)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:22:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,785
            CDSs (total)                      :: 2,751
            Genes (coding)                    :: 2,732
            CDSs (with protein)               :: 2,732
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 12 of 19
            Pseudo Genes (internal stop)      :: 5 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15243
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4696_length_15243_cov_4.643864"
                     /isolate="AMR_MDS_3064"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..617)
                     /locus_tag="P5123_04370"
     CDS             complement(<1..617)
                     /locus_tag="P5123_04370"
                     /inference="COORDINATES: protein motif:HMM:NF015451.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoacetone oxidase family FAD-binding enzyme"
                     /protein_id="HRX15527.1"
                     /translation="MKKKKIAVIGAGAAGATAAINAAREGAEVVVFEKQESLGRKIAA
                     SGNGQCNISNLEISYKNYNGDNPRFVQNIFGRFSLESTRSFFLSLGLPWRERQQGRLY
                     PYSLQARTVCDVLLDGVISAGCEIKFHRCVESISYSKNDKFTIITSGKEKYLFDSVIL
                     ACGGKSFAGLGGSDRGYNLAISLGHSVTELVPAIVPLNIHDKSLR"
     gene            801..1589
                     /locus_tag="P5123_04375"
     CDS             801..1589
                     /locus_tag="P5123_04375"
                     /inference="COORDINATES: protein motif:HMM:NF016873.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-formylglutamate amidohydrolase"
                     /protein_id="HRX15528.1"
                     /translation="MEKLPILIIIPHGGVQPPEELESLALTDKFELFLSSDTCANDLF
                     YFEKESAGVISTHISKLFVDLNRSRTAIFPATTDGVIKKKTPYGKSVYEEPFFPDEIA
                     AANIIRRYYDPFYETVDKIIKTGEIRLILECHTTFGIGLPQTPQADRPLPMITVHNRI
                     ERSGSMIQTCHDTAAVMLVELLRKNLGISPSMGTRISDTPSHGALLHTFAKYTPYLRL
                     DISRSLFFDDKYFNLEYIKVDQLRIKYLRKKIWDAISKTFLKCI"
     gene            1687..4278
                     /locus_tag="P5123_04380"
     CDS             1687..4278
                     /locus_tag="P5123_04380"
                     /inference="COORDINATES: protein motif:HMM:NF012742.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="substrate-binding domain-containing protein"
                     /protein_id="HRX15529.1"
                     /translation="MKNDRISTLTNSEQKKVSQKNHAIGVLLQEIESSYNHDILNGIM
                     KVCAKTDSDLFIFVGSALKSPHGTSERRNIIYDIAEHGDLDGYIISTATIGHYSSEKE
                     IIQFVNRFSEKPSISIGNAIEGISSIIINNYHGQKKIVEHLIKTHKCKQIAYITGGDN
                     VDSTERFKAFNDAMIENGLSVAPELVHKGDFYYFAGEQAVHSFINSGIQFDAIVAAND
                     PMALAALFELQRLGYKIPEEVKVSGFDNSSESLYSNPPLSTVTQPTFKIGKVAATELF
                     RLLNGNKKPEIIPVSTTPVIRESCGCNETLYHDSQYDIGMNYNERIKKRISEIEDIPD
                     EVIEYIQYFINNSGGLLDKFFAEDSDESEIISEFISAVYYTKPYEKVFACWTDIVSIF
                     EEYFLNTINDFDLRIRVRFLFQRLRAEISGIIQKEVADNYYYLLSFNSSSRTINEYLL
                     LSYDLENIKTTIENFLPNAGIDSAHIALYRHKEDKSGITEKITLFLSIIDGKRTELEN
                     SIITPNNLFTKLRENRNKNVVFIIEPLYFNDEDYGFIIYEHNKDHMIFYGNFTTQICS
                     VLKEYYTFSRKQDIEKELKITLNNIELVNTNLSETINSINSTKQKLIDSEKLATLGSL
                     VAGIAHEVNTPLGTTLLTTSHLSEITNAYMDLYNKNKLTKNSFENLIDKNITTGKLIL
                     DNLMKASDMINNFKQIAVDQTHFEAREFSLREYLNSIIQSLNSLIRKTSHTVTVNDGE
                     DVNINGYPGAFSQIITNLITNALTHAFENKKNGEIIISYYTKDDNITIEVSDNGCGIP
                     KENIKKIYSPFYTTKKDEGGSGLGLQICYNLVTRKMNGTIECESQIDLGTKFTVIIPR
                     DHGGAQK"
     gene            4275..6848
                     /locus_tag="P5123_04385"
     CDS             4275..6848
                     /locus_tag="P5123_04385"
                     /inference="COORDINATES: protein motif:HMM:NF012742.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="substrate-binding domain-containing protein"
                     /protein_id="HRX15530.1"
                     /translation="MKNPSSSRYNIAVLMNKVDDHYHSIVYKSIKKTVQNSDFNLIIL
                     AGRPLDSTYQFDRFHNMIFRYAQIDFFDGIIIFAGSIFNFVTKEKYLDFLNQYKHQKY
                     ISMDFEVENHPYILIDNFNAMRKETEHFIVEHGKKRIAFITGKPSSQIAVDRLNGYLQ
                     ALSDHKIPYDETLVVEGDFVSISGQKAVIELIDKRCAKFDAIIASNDEMIMGAINELK
                     KRKIKIPEDCAIAGCDNFEHVKFHNPPITSIHIPLDEQIECAFALLQDYIETDTISHE
                     IMNTKLIARKTCGCQYSPIIMKNGRNKEKNLSTNQDLIELIRSFNDIHPGVEKIFLNN
                     IEQLHEALVNDIGSCNDTLSSFETLFIQIMQNFFKDSQELFSVQKILSVFELMAFYKD
                     KEKKMESVIGETFRKLQVYCGNFFKCNTMGNNQDIIRFILNSVSIYSDIVPNEDIDQL
                     VFSIFRTCVSVNINTGHIAIFDHETGEDRTRLLLSFKDREIIKTDTHKVYSADNLLEY
                     VESHRGNRIIMALPLTCQNENYGVAFFEENDLNPAIFENLRSQISSALKGSNQFYEQA
                     LIEKELYRTVDTLEKRRNELQTRLEELNLAQRNVIEKEKNAALGNMVAGISHEINTPL
                     GICITSASHLLERSRNLLRIYKEGSLKKNDFEAHLSTVKETTRIIMDNLNRAVQIISS
                     FKQLAVDQSSNRQRKFNIKECIDDVLITLNPIFKKKAISISVNCPDTIRANNYPGALS
                     KALSELINNSLDHGLSDKNEADPGEIIITVTKKNDNAVIDYSDNGKGIPEEVLKNFFQ
                     PFVTTRRTENRLGLGLTIIHSAITRDMNGNVTYTPRQNSGTNFSLSFPLNLSKRNELT
                     S"
     gene            6970..7644
                     /locus_tag="P5123_04390"
     CDS             6970..7644
                     /locus_tag="P5123_04390"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRX15531.1"
                     /translation="MESVDYNKVVPERFRKRLNQQKNRIKLEARYDKHGRITQPFLYV
                     VGLGEKHFRNFNREILRIEKMFPWLRSKHFRFVMNELIINSQFSMLREVVSNISKGKK
                     AAGYFFVKIYPCDTFVSTSIEEFGDFFDYFSFISETGTTDERNIEAFADYKDEMHVTD
                     LNSLSDNRVKIALSCDDSLQSPDQSNRIGLNIIENATDHDFYITSFYKNKKYMWKRIH
                     FRIENN"
     gene            7651..8577
                     /locus_tag="P5123_04395"
     CDS             7651..8577
                     /locus_tag="P5123_04395"
                     /EC_number="2.7.1.26"
                     /EC_number="2.7.7.2"
                     /inference="COORDINATES: protein motif:HMM:NF004162.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003919 - FMN adenylyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional riboflavin kinase/FAD synthetase"
                     /protein_id="HRX15532.1"
                     /translation="MEIIDFNSLVKKECVLTTGNFDGVHCGHVKLLTTLNDIAQKNCY
                     KRVLLTFKRHPRLFFNPGLTHYVLSPFEEKTKLLSELVDYIVPIDFNKEFAAIEPEDY
                     MLHLIKHFNMQHYIAGYDHHIGRGKNGSFENLIKLTKKLSFKLDRVKPDIYRNKPVSS
                     SRIKDCLEQGKPEHAAIMLGRYYNLEGEVIKGRQLGRTINFPTANIGLAHDKYIPPQG
                     VYAAIAIIQDKKYPAMLNIGVNPTVTSLGQLSVEAHLPGFSGNIYGEKIKVGLINKIR
                     DEKKFKNIALLKEQIEKDKEEIIKIYYQNILD"
     gene            complement(8626..9438)
                     /locus_tag="P5123_04400"
     CDS             complement(8626..9438)
                     /locus_tag="P5123_04400"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRX15533.1"
                     /translation="MRFKNSQQINSVSSFYQLIRLLIIFMCLLMFFSYNISADDWGDS
                     NASCIDLALVMRAYDITDFSTFDSSAYIGMIKPDVAKIVGAFANTPSIDTPEELLTAL
                     FYSSILPSDDEKKETILQQISQGSAGARKIAALWFKKGADKSESFEKAIIWIAAKSSV
                     PRDSLFYFYREAISETVSQKASLLLKNAPYEYFTLPAGIASSKKEHIVKVITDYYIDP
                     VSSKEQRLIDSVNYFGKKGAAKPADMERFRMLVTLLADLSPDLKDRIEKKLR"
     gene            complement(9695..10171)
                     /locus_tag="P5123_04405"
     CDS             complement(9695..10171)
                     /locus_tag="P5123_04405"
                     /inference="COORDINATES: protein motif:HMM:NF024572.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HRX15534.1"
                     /translation="MVIKVLHFLFILLFSCTCLFAQNNSNEIERIADDARFGNAYYLV
                     TIGRKDKAITLLSEYIEVYIDGAHRKKAILTIGDIYYERFEYLRASNFYMMLFEEYPD
                     SEEGVEAYYRSGLCFIKMGDRDKAAAVFQDIVENYQGFAASEKAGLEYTINTLIEE"
     gene            10571..10996
                     /locus_tag="P5123_04410"
     CDS             10571..10996
                     /locus_tag="P5123_04410"
                     /inference="COORDINATES: protein motif:HMM:NF012383.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosyltransferase"
                     /protein_id="HRX15535.1"
                     /translation="MDHIRVDYNKFIELGYKIVDQIKKSGVKYDEILCPLRGGYMLSY
                     LVSNNLKIPVNFMEISSYNGKEQREFKIGHKPDLAHGKYLLCDDIYDSGKTIKKILEI
                     YQGIDMDVACLISKYPVEKYYVGEFIEDDRWIDFFWETM"
     gene            11010..11567
                     /locus_tag="P5123_04415"
     CDS             11010..11567
                     /locus_tag="P5123_04415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013930176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRX15536.1"
                     /translation="MKDFLRKHRFETVLFLIFFALISRLAPHPSNFTPLTAVALFSAA
                     FIKRKSLAFIIPVAGFFASDMILNLVKSDYNYGIINLIMYLVIILIASTGVLLGRKVT
                     IIRTALFSISGSSLFFIITNFAVWFFWDMYPKTVSGLFSCYTAGIPFFRNMVAGDLVY
                     TGLLFGSFALVARYSENREVALSDC"
     gene            complement(11564..12142)
                     /locus_tag="P5123_04420"
     CDS             complement(11564..12142)
                     /locus_tag="P5123_04420"
                     /inference="COORDINATES: protein motif:HMM:NF013702.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-acyl-sn-glycerol-3-phosphate acyltransferase"
                     /protein_id="HRX15537.1"
                     /translation="MRGLCRFFITAKIKMGQIFFYLILRLLRWNIDRAPIDKDRGAVL
                     AFFPHESNFDAILGFLTFKINGLHPKTLAKSELFWFPFGIIMRFLGGIPVYRSSSSDM
                     VSQMVKRFNEDPCFFLAIAPEGTRKRAETVKSGFWYIAKGANVPIHLFLIDKKNRRIR
                     RLETLYPQGTYEEDIKKIANIYSKSGYKLPGF"
     gene            complement(12166..13626)
                     /locus_tag="P5123_04425"
     CDS             complement(12166..13626)
                     /locus_tag="P5123_04425"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HRX15538.1"
                     /translation="MLNTIFYSGLLLLFLGLTEIGIFINIHKSTLERLDRDQSYALRL
                     VYNQFMSNITRVDFEIGMVSAAVSSNDHISKKTLLGSFNQPLLNFMVNDRGYYYKIIF
                     YDNHGKKLISFEPSAKKKSHSKTGSCSMLQQTNLLFSHDIIGYDSKIAGTIIIVVPLH
                     LIMNSLDSVNRAENGDIYIVDTNGNLIYSSGTCTVQNSVNEVVDYLFSEYEKNENIAY
                     MEDVRAFNNGGGFFHLTTINPLKMKNSHVSFVNNPKPYLSDSGKHLVDSSKLLLFYHL
                     PPALANKADELLVKALLLGAIVLTPFLVLLAFFLAKGRVRNHYYLLELKESATIDKLT
                     GLFNHRYMDGQLYSQSKMALRRGSKLTLAYIDVNDLKLTNDRYGHAEGDQMLIGAARA
                     FAASIRSTDIAGRIGGDEFLIIFPDCSLQRAELIMERITENFKSLGFKHNNQAWTLSY
                     GCSEWLGENDTVQNLLSRADSIMYNNKKKHKNSKTS"
     gene            13845..14912
                     /gene="leuB"
                     /locus_tag="P5123_04430"
     CDS             13845..14912
                     /gene="leuB"
                     /locus_tag="P5123_04430"
                     /EC_number="1.1.1.85"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014456763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003862 - 3-isopropylmalate dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0009098 - leucine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydrogenase"
                     /protein_id="HRX15539.1"
                     /translation="MKNYNIAVLPGDGIGPEVMAEAIKILDVVAKTENITLNYTHAEV
                     GGAAIDNHGKALPESTLDTCKTSDAILFGSVGGPKWETLPPDEQPERAALLPLRKIFN
                     LYANLRPAIIFPALKEASPIKSEIIGDGFDILVIRELTGGIYFGQPKGIEADRAFDTL
                     VYTRPEIERITHVAFQAAMKRGKKVVSIDKANVLNSMVFWREIVAGIAKEYPEVELTN
                     MYVDNAAMQLVKNPKQFDVILCGNMFGDILSDEASMITGSLGMLPSASLSEGSFGLYE
                     PAGGSAPDIAGKGIANPIAQILSAAMMLKYSFGMDNAYDNIFNAITAVLNDGLRTGDI
                     FSEGCKKVNTSEMGDAIVAKL"
BASE COUNT         5170 a         2922 c         2628 g         4523 t
ORIGIN      
        1 tgacgcagtg atttatcgtg aatatttaga ggtacaatgg cgggaacaag ctctgttaca
       61 ctatgtccaa gagagatagc aaggttatat cctctatcag aaccaccaag ccctgcgaaa
      121 cttttacctc cgcaagctag aataaccgaa tcaaagagat atttctcttt gccggatgta
      181 ataattgtaa atttatcatt tttcgaatac gaaatacttt caacacatcg atgaaacttt
      241 atttcacaac cagcgctaat gactccatct aacaacacat cacaaacagt tcgagcctgt
      301 agcgaatatg ggtagagccg cccctgttgc ctctccctcc agggtaaccc aagagataaa
      361 aagaaggagc gggtactttc cagagaaaaa cgaccaaaaa tattttgtac aaaccttggg
      421 ttatctccat tataattttt atagcttatt tcaagattac taatattaca ttgcccgttt
      481 ccactcgcgg caatctttcg acctagagac tcctgttttt caaacacaac gacctccgct
      541 ccttcacgag cggcgttaat tgcagctgta gcccccgctg cacccgcacc gataacagca
      601 attttctttt ttttcatcat aaactcccac gaataagcca aaaagtatat tagttataac
      661 agaaaacttt atttaacgtc ttgcaccaca tagcaaagac acctttacta ttcaacaaaa
      721 agaactttga atgtttgcca attgaaaaaa cattcacaaa agctgtttga caaattatta
      781 taaagagaca taatatctca atggaaaaac tgccaatact tataattatt cctcatggtg
      841 gggttcaacc tccagaagag ctagaatcgc tggccttgac agataaattt gagctatttt
      901 tatcctcaga tacctgtgcc aacgatcttt tttacttcga aaaggagtcc gcaggagtaa
      961 ttagcaccca tatatccaaa ctttttgtgg atctaaaccg ttcgagaacc gcaatttttc
     1021 cagctacaac cgacggtgtg attaaaaaaa agactccgta cggaaaatct gtttacgaag
     1081 aaccgttttt ccccgacgaa attgctgccg caaatataat tcgccgttat tatgacccgt
     1141 tttatgagac agttgataaa ataattaaaa ccggagagat tagattaatt cttgaatgcc
     1201 ataccacctt tggtattgga ttaccccaga ctccacaggc agatcgccca ttacctatga
     1261 ttactgttca caatcgtata gaacgcagtg gcagcatgat tcagacatgt catgacactg
     1321 ctgcggtgat gctggttgaa ttattgagaa aaaacctggg aatatcacca tctatgggaa
     1381 cacgaatatc cgataccccc tctcatggag cacttctaca tacattcgcg aaatacaccc
     1441 cctatctaag gcttgatata tcacgctctc tcttttttga cgacaagtat tttaacctgg
     1501 aatatatcaa ggttgaccag ctaaggataa aatatttaag aaaaaaaata tgggatgcta
     1561 taagcaaaac attcttaaaa tgcatctaat caagccgggg catcttatca aacagaattg
     1621 catctcaaaa atattgcagc ttttatcaga tattgatata ttggtatcag aggcgcgatt
     1681 gacaaaatga aaaatgaccg aatatcaaca cttacaaact ccgaacaaaa aaaagtttcc
     1741 caaaagaacc atgctattgg ggtactatta caagaaattg agagcagtta taaccatgac
     1801 atattgaatg gaatcatgaa agtctgtgca aaaactgatt ctgacctctt tatttttgtt
     1861 ggaagcgctc ttaaatcacc tcacggaacc tctgaacgac gcaatataat atacgatata
     1921 gccgaacatg gtgatctgga cggctatata atatcaactg ccactatcgg ccactactca
     1981 tctgaaaagg agattataca gtttgttaac cggttctcag aaaagccatc tattagtata
     2041 ggcaacgcta ttgaaggaat ttccagcatt ataataaata attaccatgg tcaaaaaaag
     2101 atagtcgaac atcttatcaa aacacataaa tgcaaacaaa ttgcctatat taccggcggc
     2161 gataacgtcg actctactga acgttttaag gcttttaacg atgcaatgat agagaatgga
     2221 ttatcagtag cacccgaact tgttcataaa ggcgatttct actattttgc aggcgaacag
     2281 gccgtacact cttttatcaa ttcaggaatt caatttgatg ccatagtcgc tgcaaatgat
     2341 ccaatggctc ttgctgcact tttcgaactg caacgattag gttacaaaat cccagaagag
     2401 gtgaaggtat ccggatttga taacagctct gagtctcttt atagcaaccc gccactttcg
     2461 actgtaactc agccaacttt taagattgga aaggtggcag ctacagagct cttcaggcta
     2521 ttaaatggca ataaaaagcc ggaaataata ccagtttcca caacacctgt aattagagaa
     2581 tcttgcggat gcaatgaaac tctgtatcat gacagccaat atgatatcgg tatgaattat
     2641 aacgaacgca tcaagaagag aatatctgaa atagaagata ttcctgacga ggtaattgaa
     2701 tatattcaat attttataaa taatagcgga ggtcttctgg acaagttttt cgcagaagat
     2761 tcagatgaat cagagataat aagcgaattt atatccgcag tctattacac aaaaccatat
     2821 gagaaagtct ttgcgtgctg gacagacatt gtttccattt ttgaagaata ttttttaaat
     2881 acaattaatg actttgattt gcgtattcga gtcagatttc tgtttcaaag acttcgcgca
     2941 gaaatttctg gaatcattca aaaggaggta gctgataatt attattacct tctttctttt
     3001 aattccagtt ctagaactat taatgaatat cttttattat cttatgactt ggaaaacatc
     3061 aagacaacga tagaaaattt tttaccaaat gccggaatag attccgcaca tatcgccttg
     3121 taccgtcaca aagaagataa aagcggtatc accgaaaaaa taactctttt tttgagcata
     3181 attgacggaa aaagaaccga gctcgaaaat agcataatca caccaaacaa tctttttaca
     3241 aaactcagag aaaaccgtaa caagaacgtt gtttttatta tagaacctct atattttaat
     3301 gatgaagatt atggtttcat tatttacgaa cacaataaag accatatgat tttttacgga
     3361 aacttcacaa ctcagatatg cagtgtgctt aaagaatatt acacattttc acgaaaacag
     3421 gatatcgaaa aagaattaaa gattacactt aataacattg aacttgttaa cacaaatctt
     3481 tctgaaacga taaacagcat taatagtact aaacaaaaac tcatagactc tgagaagctg
     3541 gccaccttag gatctcttgt ggcgggaatc gcgcatgaag taaacactcc actaggaacg
     3601 accctcttga caacatctca cctgtcagaa ataaccaatg catatatgga tctgtacaat
     3661 aaaaacaaat taacaaagaa tagtttcgaa aatctgattg ataagaatat tacaacgggc
     3721 aaactgatac ttgataattt gatgaaagct tctgatatga taaataattt taaacagatc
     3781 gcagttgatc aaacacattt tgaagcaaga gaattcagtc tgcgcgaata cttgaatagt
     3841 attattcaaa gtcttaactc cctaattcga aaaacaagtc atacagtaac ggtaaacgat
     3901 ggtgaagatg taaatataaa tggttaccct ggagcctttt cacaaataat tacaaactta
     3961 attacaaatg cactgactca tgcgtttgag aacaaaaaaa atggcgaaat aatcatctct
     4021 tattacacaa aggatgacaa tattaccata gaagtttcgg ataatggatg cggcattcca
     4081 aaagaaaaca taaaaaaaat atactctccc ttttatacga ctaaaaagga tgaaggcggt
     4141 agcggactgg gtttgcagat atgctataat cttgttacac gtaaaatgaa tggaaccata
     4201 gagtgtgaaa gccaaattga tttaggtacg aagtttacag taataattcc ccgagatcac
     4261 ggaggagctc aaaagtgaaa aatccatctt catccagata caatattgct gttttgatga
     4321 acaaggtaga cgaccactat cacagtatag tttacaaaag tataaaaaaa accgtacaaa
     4381 attccgactt taacctcatt attcttgcag gaagacctct ggattcgacg taccagtttg
     4441 accgttttca taacatgata ttcagatatg ctcaaattga tttttttgat ggaataatta
     4501 tctttgcagg ttcaattttc aactttgtca caaaagaaaa gtatctagat tttcttaatc
     4561 aatataaaca tcaaaaatat attagtatgg attttgaagt tgagaaccac ccatacattc
     4621 tgatagacaa ctttaacgcc atgcgtaaag agactgagca ttttattgtt gagcatggaa
     4681 aaaaacgaat agcttttatt acaggcaaac cctcaagtca aattgctgta gataggttaa
     4741 atggctatct tcaggcatta tctgaccaca agatacccta tgatgaaacg ctagtagttg
     4801 aaggtgattt cgtctcgata tcaggtcaaa aagctgtaat cgaacttata gacaaacgtt
     4861 gtgcgaaatt tgacgctata attgcttcca acgatgaaat gataatggga gcaattaatg
     4921 aattaaaaaa acggaaaata aagattcctg aagattgtgc catagccggt tgtgacaact
     4981 ttgaacacgt caagtttcac aatccgccta tcactagcat tcacataccg ctggacgaac
     5041 agatagagtg cgcttttgcg ttgcttcaag attatattga aactgatact atttctcatg
     5101 aaataatgaa caccaagcta attgcgcgta aaacctgtgg ttgtcaatat tcaccaataa
     5161 ttatgaagaa tggtcggaat aaggaaaaaa atctttcaac caaccaagat ttaattgaat
     5221 taatcagaag ttttaacgat atacaccccg gtgttgagaa aatttttctc aataacattg
     5281 agcaactaca tgaggctctt gtaaacgata taggatcctg taatgacaca ttatcttctt
     5341 ttgagacctt attcatacaa ataatgcaaa attttttcaa agacagtcag gaactgtttt
     5401 cggttcaaaa aattttgtca gtttttgaac taatggcttt ttataaagat aaagaaaaga
     5461 agatggaatc agttattggc gaaacattca gaaaacttca ggtctattgt ggtaattttt
     5521 ttaaatgtaa caccatgggc aataaccagg atataatccg atttattctt aattctgtct
     5581 caatatatag cgatattgta cctaacgaag acatcgatca gctagttttt tcgattttta
     5641 gaacctgcgt ctcagtaaat ataaatacag gccatatcgc aatttttgat catgaaacag
     5701 gcgaagatag aacaaggttg ctactttcgt tcaaagatag agagattatc aaaactgata
     5761 ctcacaaagt atacagcgcc gacaatctgc ttgaatatgt tgaaagccac cgagggaacc
     5821 gaattattat ggctctacca ttaacctgtc aaaatgaaaa ctacggtgta gcttttttcg
     5881 aagaaaacga cctgaaccca gcaatatttg aaaatttaag atctcaaata tcaagcgcgc
     5941 ttaagggctc aaatcaattt tacgaacagg ctcttattga aaaagagctt tatcgcactg
     6001 ttgatacttt ggaaaagcga agaaatgagc tacagacccg tcttgaagaa cttaatctgg
     6061 ctcaaagaaa tgtaattgag aaggaaaaga atgctgcttt aggcaatatg gtagcaggaa
     6121 tatcacatga gataaacacc cctcttggca tatgcataac cagtgcatct catcttcttg
     6181 agagatctcg taatctttta agaatttaca aagaaggtag tctgaaaaaa aatgatttcg
     6241 aggcgcatct ttccacagtc aaagaaacaa ccagaataat catggataat cttaataggg
     6301 cagtccagat tataagcagc ttcaaacaat tagctgttga ccaatcaagt aacagacaac
     6361 gtaaattcaa cattaaagaa tgtattgatg atgtattgat tacactcaat cctattttta
     6421 aaaaaaaggc tatttcgata agtgtgaact gccccgatac gatcagagca aacaactatc
     6481 caggagccct ttccaaggct cttagtgaac taataaataa tagccttgat cacggcttat
     6541 ctgacaaaaa tgaagccgat ccaggagaaa ttattatcac agtcactaaa aaaaatgata
     6601 atgctgttat cgattacagc gacaacggaa aaggtatacc agaagaagtt ttaaaaaatt
     6661 tttttcaacc ttttgtgaca acacgtcgaa cagaaaacag actgggatta ggactaacaa
     6721 taattcacag tgcaataaca cgcgatatga acggtaacgt cacctatact cccagacaaa
     6781 acagtgggac aaatttttcg ctatcatttc ctctaaattt atcaaaaaga aacgagctga
     6841 caagttgact tctctaattt ctttttattg atatatcaat attgtacttg atgaaaatgt
     6901 acagttagca caaaatgagc aaaattgcaa attatttgca aataaacaga tacatacggt
     6961 gatacatcaa tggaatccgt cgactacaac aaggtagttc ctgaacgttt tcgtaaaaga
     7021 ctcaatcaac aaaaaaacag gatcaaactt gaagcccgtt atgacaaaca tggacggatc
     7081 acacaaccct ttctttatgt tgttggcttg ggagaaaaac actttcgcaa ttttaaccga
     7141 gagattttgc gaattgaaaa aatgtttcca tggttacgat cgaaacattt tcggtttgtt
     7201 atgaacgagc tgattataaa ctcacagttt tcaatgctac gcgaagttgt tagcaatatt
     7261 tccaaaggga aaaaagctgc cgggtatttt tttgtaaaaa tttatccatg tgatactttt
     7321 gtatcaacaa gcattgaaga atttggcgat ttctttgatt atttttcatt catctctgaa
     7381 accggcacaa ctgatgaacg gaatattgaa gcatttgccg attataaaga cgaaatgcat
     7441 gtaaccgacc tgaatagctt atcagataat agagtaaaaa ttgcactttc atgcgatgac
     7501 tcattacaat ctcctgacca gtcaaacaga ataggattaa atattattga aaacgcaaca
     7561 gatcacgatt tttatataac ttcattttat aaaaataaga aatacatgtg gaaacgaatt
     7621 cattttagaa tagaaaataa ctgaagtcat atggaaataa tagattttaa ttcactcgtt
     7681 aaaaaagaat gtgttcttac aaccggaaac tttgacggtg tacactgcgg ccacgtgaaa
     7741 cttcttacga ctttaaatga tatagcccaa aaaaactgtt ataaaagagt tctactcact
     7801 ttcaaaagac atccccgcct tttttttaat cccggcctta cccactatgt cctttcacct
     7861 tttgaagaaa aaaccaaatt actctcggaa ctggtcgact acatagtacc cattgatttt
     7921 aataaagagt ttgctgcgat tgaacctgag gattatatgc ttcatctaat taaacacttt
     7981 aatatgcagc actatatcgc cggatacgac catcacatcg gaagagggaa aaacggatcg
     8041 ttcgaaaatc taataaagtt aacaaaaaag ttatctttta aacttgaccg tgtaaaacct
     8101 gatatttatc gaaataaacc cgtgtcttct tcacgaataa aagattgcct tgaacaaggg
     8161 aaacccgagc acgcggcaat aatgttagga cgatactata atcttgaagg cgaagtaatc
     8221 aaaggacggc agcttggtag aacaataaat ttccccacag caaatatcgg tttggctcat
     8281 gacaagtata ttcctccaca gggcgtttat gcagctattg cgattattca agacaaaaag
     8341 tatccagcaa tgttgaatat tggagttaac cctaccgtca cgagcttagg gcaactttct
     8401 gttgaagcgc acttacccgg attttcgggc aatatttacg gcgaaaagat taaagtgggg
     8461 ctgataaata aaatcaggga cgaaaagaaa tttaaaaata tagctctgtt aaaagaacaa
     8521 atagaaaaag ataaagaaga gattataaaa atttattatc aaaacatttt ggactgatac
     8581 tgtttctttg cctcacgata cacgatattg atgctccacc aataattatc tcagtttttt
     8641 ctctatgcgg tcttttaaat cgggagagag atcagctaat agagttacta gcatacgaaa
     8701 acgttccata tccgcgggtt tagcagctcc tttcttaccg aaatagttaa cagaatctat
     8761 taacctctgt tcttttgaac ttactgggtc aatataataa tcagttatca ctttaactat
     8821 atgctctttt ttcgatgacg ctattcccgc cggcagtgta aaatattcat agggagcatt
     8881 ttttaataat agcgacgcct tttgtgaaac tgtctctgaa atagcttcac gataaaaata
     8941 gaaaagcgaa tcacgaggaa cagaactttt agccgctatc catataatag ctttttcaaa
     9001 agattcagac ttgtcagcac cctttttaaa ccagagagcc gctatcttac gcgcaccagc
     9061 acttccttgg gatatctgtt gaagtattgt ctcttttttt tcatcgtcag aaggcaaaat
     9121 agacgagtaa aacagtgcgg ttaataattc ttctggggta tcgatgctag gtgtatttgc
     9181 aaaagctccg actattttcg ctacatccgg ctttatcata ccgatataag ctgaagaatc
     9241 gaatgttgaa aaatctgtta tatcataagc tctcataacc agtgctaaat caatgcaaga
     9301 tgcatttgag tctccccaat catcggctga aatattataa ctaaagaaca ttaagagaca
     9361 catgaagatt atcaataacc taatcagctg gtaaaatgaa gagacgctat ttatttgctg
     9421 agaatttttg aatcgcatta tcttgccacc atttttattc cgacctatta cggaaattta
     9481 accgttttta tgatgtgaac ttaagaagca taactactgg ataaaccttc tcaaacctca
     9541 ccgatatagt agttataaaa atgataattt acgaagctca ctaataacat ttattctgat
     9601 tttccctaaa aaacttacaa gtatcacttt atacagacta ccttcttagc gaacatataa
     9661 aatagaatct tctataaata attaatgttt taaactactc ttctattaat gtgtttatcg
     9721 tatactctaa tcccgctttt tcagaagcgg caaatccctg ataattctcg acgatatcct
     9781 ggaatacggc tgcggcctta tcacggtcac ccattttaat aaaacacaaa cccgatctgt
     9841 aataggcctc tacaccctct tcagaatcag ggtactcttc aaaaagcatc atgtaaaaat
     9901 tagaagctcg aaggtattca aaacgctcat aataaatatc acctatggtt agaattgcct
     9961 ttttacgatg cgctccatca atgtatacct caatatactc acttaaaaga gttatagcct
    10021 tatcttttcg ccctattgtt actaaataat aggcatttcc aaatcgggca tcatcagcta
    10081 tacgctcaat ttcattactg ttattttgag caaaaagaca tgtacaagag aacagaagaa
    10141 taaacagaaa atgcagaact tttattacca tgtcatcacc aacttttacc tgaaaaccaa
    10201 aatgatcctc tgataaccaa atttcatcat taaactttcg taaaatcaca attgaacagg
    10261 tacttaaaca atgattacct cgaaaagtga tatttcgact ttatttacca taaattactt
    10321 tattatacat atagcaattg agcaattcaa aataaatatt gcaatcgtgt aatacaccaa
    10381 caaaattaca ataatttcag actttaagat attacatata tattatttcg gcaattatgt
    10441 ctcatttgat aagctttttt tggctaacaa tccaaaacag ttttaattaa tattgtttac
    10501 gatttttagt catgctatac ttatggaaac ggattttatc tgcatctttg gctttcggag
    10561 tatattgaaa atggatcata tcagagttga ctacaacaaa tttatcgaac ttgggtacaa
    10621 gatcgttgat cagatcaaaa aaagcggtgt gaaatacgat gagattcttt gtcccttacg
    10681 tggcggctat atgctctctt accttgtatc taacaatctg aaaatacctg tcaactttat
    10741 ggaaatttct tcatacaacg gaaaagagca gagagaattc aagataggac acaaacccga
    10801 tcttgcacat ggaaaatacc tgttatgtga cgatatttac gactcgggca aaaccattaa
    10861 aaaaattctt gagatttatc agggtattga tatggatgta gcctgtttga tttctaaata
    10921 tcctgtcgag aagtactatg ttggcgagtt tattgaagat gaccgctgga tcgacttctt
    10981 ctgggaaacc atgtaaaccg gagaccgtaa tgaaagattt tttacgtaaa catcgatttg
    11041 aaacagtttt gtttctaata ttttttgcgc taatttcgcg ccttgctcca catccatcga
    11101 actttactcc attaacagca gttgcccttt tttcggctgc ttttattaaa cgcaagagcc
    11161 ttgcctttat tattcctgtt gcgggctttt ttgcttcaga tatgattttg aatctggtaa
    11221 aaagtgatta taattacggc attataaacc ttataatgta cttggtcata atacttatcg
    11281 cttccacagg ggttttactt ggaagaaaag taaccattat tcgcacagct cttttctcaa
    11341 tttccggatc atcactattt tttattataa ccaattttgc ggtctggttt ttttgggaca
    11401 tgtaccccaa aacagtatcg ggtcttttta gctgctacac tgcaggaatt ccatttttta
    11461 gaaatatggt ggccggtgat ttggtctata caggacttct tttcggctca tttgctcttg
    11521 tcgcgcgtta cagcgaaaac agagaggttg ccttatctga ctgttaaaac ccaggcaact
    11581 tatagccgct tttagagtag atattcgcaa tttttttaat atcctcctca taggttcctt
    11641 gtggatatag agtctcaagc cgccgaattc ggcggttttt tttatcaatc agaaagagat
    11701 gtattggcac atttgcacct ttagctatgt accagaaacc acttttaacc gtttccgctc
    11761 ttttccgtgt gccctccggg gctatggcta agaagaaaca cggatcctca ttaaaacgtt
    11821 taaccatttg tgataccata tccgaagatg acgaacgata aaccggtatg cctcccaaaa
    11881 atcgcataat aataccaaat ggaaaccaga aaagctcact tttagcgaga gtctttggat
    11941 gcagaccgtt tattttaaag gttagaaaac ccagaatagc atcaaaattt gattcatggg
    12001 gaaaaaatgc aagaacggca cctctgtctt tatcgattgg cgccctatca atattccatc
    12061 ttaatagtcg caatatcaga taaaaaaaaa tttgccccat tttgattttt gcagttataa
    12121 aaaaacgaca aagccctctc attgcagata gacactccta aataatcagc tagttttact
    12181 atttttatgc ttttttttat tattatacat tattgaatcg gcacgcgaga gtagattctg
    12241 aactgtatca ttttctccca gccactcaga acatccatag ctaagagtcc aggcctggtt
    12301 attatgttta aaacccagag atttgaaatt ttcagtaatt ctttccataa taagctcagc
    12361 cctctgtaga gaacaatccg ggaaaataat caaaaactca tcgccaccta tgcgccctgc
    12421 tatatcggta gatcttatgc ttgccgcaaa agctctggcc gcaccgataa gcatctgatc
    12481 tccctcggca tgaccatatc tgtcattagt aagttttaaa tcattaacgt caatataagc
    12541 caaggttaat tttgatcccc gccgaagagc cattttgctc tgactataca attgaccatc
    12601 catataacgg tgattaaaca gtcccgtaag tttatcgatg gtagcgctct cctttaactc
    12661 cagcagataa taatggtttc taaccctgcc ttttgcaaga aaaaaggcaa gcaaaaccag
    12721 aaacggagta agcactatgg cccctagaag caaagccttg actaaaagct catctgcttt
    12781 atttgcaaga gccggaggca agtgatagaa caataacaat tttgaactat ctaccagatg
    12841 cttaccagaa tcagatagat acggtttcgg attattaaca aatgatacat gactattttt
    12901 cattttaagc ggatttattg tggtcaaatg aaaaaaacca ccgccattgt taaaggctct
    12961 cacgtcttcc atataggcta tattctcgtt tttttcatat tctgaaaata aataatctac
    13021 tacttcgtta acactatttt gcactgtgca agtaccagac gaatatataa gattaccgtt
    13081 tgtatcaaca atataaatgt caccattttc tgctcgatta actgaatcaa gactgttcat
    13141 tatcagatgc aatggaacaa ctataattat tgttccagct attttgctgt cgtatccaat
    13201 tatatcatgg ctaaaaagaa ggtttgtttg ttggagcatg gaacaagagc ctgttttact
    13261 gtgagacttt ttctttgcac tgggttcaaa agagatcagt ttttttccat gattatcata
    13321 aaagattatt ttatagtaat agcctctatc gttaaccata aaatttaata gaggctggtt
    13381 aaaactacct aatagtgttt ttttgctaat atggtcatta gaggaaaccg cggctgaaac
    13441 cataccaatc tcgaaatcaa cacgcgttat gttagacata aactgattgt ataccaacct
    13501 taacgcgtag ctttggtcac gatcaagccg ttcaagagtt gatttatgaa tatttatgaa
    13561 tatccctatc tcggttaggc caagaaacag cagaagtagc ccagaataga aaatagtgtt
    13621 aagcatcaca tttcgtgaaa aaagtttatc ccgagacttg tgatgtttat gttttataga
    13681 aaatgatatc ttcatgtaaa ataatataat aatttcggtg ttaaaattta cccaaaatta
    13741 aaaaaaatcc gaatattttg cagctcggtc acaaaattat tagttgacat gaactttacc
    13801 tttactaaag tatctttatt atttataaag attggaggtt tgaaatgaaa aactataata
    13861 tagccgttct tccaggagac ggtattggtc cggaagttat ggctgaagcc attaaaattc
    13921 ttgatgtagt agccaaaaca gaaaatatta ctcttaacta tacacacgcc gaggttggtg
    13981 gcgccgcgat tgataatcac ggcaaggcat tgccagaaag cactcttgat acctgcaaaa
    14041 cgtctgatgc cattcttttt ggatcggttg gtggtccaaa atgggagaca cttccacccg
    14101 acgagcaacc tgaacgcgcc gcacttttgc cacttagaaa gatatttaat ctttacgcga
    14161 atctgcgacc ggctattatc tttccagctt taaaagaggc atcaccaata aaatctgaaa
    14221 taatcggaga cggttttgat attcttgtta ttcgcgagtt aaccggaggt atctactttg
    14281 gccagcctaa gggaatagag gccgaccgcg ccttcgatac acttgtttac acaagacccg
    14341 aaattgaaag aattacacat gtagcgtttc aggctgcaat gaaaagagga aaaaaagttg
    14401 tctctataga caaagccaat gttttaaact cgatggtttt ctggcgagaa attgtggccg
    14461 gtattgccaa agagtaccct gaagtggaac tgacaaacat gtatgtggat aatgccgcga
    14521 tgcagctcgt gaaaaacccg aaacagtttg acgtaatact ttgcggaaac atgttcggcg
    14581 atattctgtc tgatgaagca tcaatgataa caggttcact gggtatgcta ccttcggctt
    14641 cattgtcgga aggaagtttt ggtctttacg aaccggcagg cggctcggct ccggatattg
    14701 ccgggaaagg tatagctaac cctatcgccc agatattatc cgcggcaatg atgttaaaat
    14761 acagctttgg aatggataat gcttacgata atatatttaa cgcgattaca gcagttttaa
    14821 atgatggatt acgtaccggt gatattttct ctgaagggtg caaaaaagtt aatacttccg
    14881 aaatgggcga tgccatagtc gcgaaacttt agataaacag atcatgatgc cgggttgcat
    14941 ctgcagctcg gtaatcatag cacagatttg acaaacaata aagacactca atttcgccac
    15001 ctgctccatc atggaatagc cgttaatccg ctgagtagag tatctcacct ggttgtacaa
    15061 atttataaat ctcaattaaa cagcaattct atttaatcgc agttatctca tcttcagcaa
    15121 gaccggtaca gcgggatatc atttctatag aaacaccctc tttaagcaat tctttggctg
    15181 tttcaagttt accttcaagt ttaccctcaa tcttaccctc ttctcttaat ttcatcgcgg
    15241 ttg
//
LOCUS       DAOTGZ010000042        15151 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_3064
            NODE_4726_length_15151_cov_5.890700, whole genome shotgun sequence.
ACCESSION   DAOTGZ010000042 DAOTGZ010000000
VERSION     DAOTGZ010000042.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868088
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 15151)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 15151)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:22:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,785
            CDSs (total)                      :: 2,751
            Genes (coding)                    :: 2,732
            CDSs (with protein)               :: 2,732
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 12 of 19
            Pseudo Genes (internal stop)      :: 5 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15151
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4726_length_15151_cov_5.890700"
                     /isolate="AMR_MDS_3064"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..634
                     /locus_tag="P5123_04435"
     CDS             <1..634
                     /locus_tag="P5123_04435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010419457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="flagellin"
                     /protein_id="HRX15540.1"
                     /translation="VQTAEGYLDQSASILQRIRVLAVQSSNGIYTDNDRQLIQVEVSA
                     LIDEVDRIASQAEFNKFKLLLGDFSRTNPRASMWFHMGANMHQRERVYIGTMTSEGLG
                     LTERTGEFLANLSTADKANRTIGIIDDALHKVSKQRADLGAYYNRLELTSKGLMTAYE
                     NIQASESRIRDADMAETMVELTKDQVLVQSGTSMLAQANQKTQTVLQLLR"
     gene            1122..2258
                     /locus_tag="P5123_04440"
     CDS             1122..2258
                     /locus_tag="P5123_04440"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRX15541.1"
                     /translation="MKRLVFTVVALLLWIVFLMAIFFDDIVLFVYFIKERDKVISDQM
                     LNYENLYLSGDDHAIYNNSTIHVSSSFTLSGDSLLEIYNSKIVITPFYDNLFEMDIKD
                     NSRMVIRNCEVTTSEGSVQYCKIYDHASVEVIDVQNDGLNYILSDSSEIKIYKSVSDI
                     SISAGAKPDILIDRSPYISLLYVLDSAEKVSDIPANEFVEEYNNELFGHLEINKSFIQ
                     SVGFMIGSSVNLTVKDCNAVELIWKNESQNEILVEGFKQQMYKDKYFEFGESKVLLLN
                     SRVVRSVINNDGGGELIIRDSDLTTASVSGDGILSGERLRVDYLVISDESAAILYDSA
                     VSVLADIADRANLFIENSEVSDYTFSRRVSDFYFYIDGEKSNKR"
     gene            2287..2655
                     /locus_tag="P5123_04445"
     CDS             2287..2655
                     /locus_tag="P5123_04445"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRX15542.1"
                     /translation="MDIEYKYNELKGIIENMESVDDSSIIMKIADRLHGISYSPPFLI
                     EVKHFTEMDKKSAITALNYIFEFPESEIKKFAISRDKSVAEIKIDAMNGFVAEYGLLQ
                     RLRMDVPEAWDQINELYEDD"
     gene            2721..3101
                     /locus_tag="P5123_04450"
     CDS             2721..3101
                     /locus_tag="P5123_04450"
                     /inference="COORDINATES: protein motif:HMM:NF013629.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor"
                     /protein_id="HRX15543.1"
                     /translation="MERKSRQKTEIRKILSQNNFHPTIEEIHLILKKRVPGVGIATVY
                     RNIDKMVRNGEIIRLEVPGQGARFDGNTEKHYHFRCEKCNSVEDVWLDIDINSYIKTV
                     SPLKESIIRDHMIEFTGICAECQK"
     gene            3195..3575
                     /locus_tag="P5123_04455"
     CDS             3195..3575
                     /locus_tag="P5123_04455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011917559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004784 - superoxide dismutase activity
                     [Evidence IEA];
                     GO_process: GO:0019430 - removal of superoxide radicals
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="desulfoferrodoxin"
                     /protein_id="HRX15544.1"
                     /translation="MMKRLYVYKCNVCGNVVELMSVGGGTLSCCGEDMHYMEEKSADS
                     STEKHVPIIEPIDEGVRVTVGSTEHPMTDEHYIQWIEVINGDWVNRKYLAPGQKPQAE
                     FYVPFSDTLVAREFCNVHGHWKNK"
     gene            complement(3703..4083)
                     /locus_tag="P5123_04460"
     CDS             complement(3703..4083)
                     /locus_tag="P5123_04460"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRX15545.1"
                     /translation="MKKIHKNIFESAAPYLVLMLTLNIVIIAGQLIFLYGTLTSFIIT
                     VIFFIFIIITAFGLYLRKPKSRIIFLIICSIYSGVAIPSSIFSIINGSTRLKLFFSIY
                     NLILAVIYLLHILITTKPQMQSID"
     gene            4270..4761
                     /locus_tag="P5123_04465"
     CDS             4270..4761
                     /locus_tag="P5123_04465"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRX15546.1"
                     /translation="MLRLSLVRHAKSSWKNDLLSDHDRPLKTRGISDSGMIAQKISDL
                     GLIPPVIFCSTALRSQLTVQLMNEIFALSEEQIRYDQVLYRAWADSILDYVKKEAKND
                     HIMVVGHNPGVHQLVEKLTDSRIEKFQTCAYASIVFDCNSWSDVQDGRGKLDHYIYPR
                     MFY"
     gene            4864..5808
                     /locus_tag="P5123_04470"
     CDS             4864..5808
                     /locus_tag="P5123_04470"
                     /EC_number="5.4.99.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006570350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HRX15547.1"
                     /translation="MENEVIQIPSSESAISERLDKFLADNCSCNFSRSYILKIINQGL
                     VSCGGNVLKASYRVKADDVFDLVLPKLEKLDLSPQDIPLDVVYQDSDIAVINKPAGLI
                     VHPGAGAPKDTLINALLYHLDGLSSIGGVERPGIVHRLDRDTAGLMVIAKNDNAHISL
                     SSQFEKRTVSKVYTALVKGKPRIANFIIENKIERHPKYRQKMTVSSKGRLAKSEITVK
                     KVWDCKDGTFSLLKVRIFTGRTHQIRVHLSSEGLPIIGDSVYSKSSQKYRLPYLLLAS
                     VHLGFDHPVSGDRMEFDAELPEHFLKFIEKLDRSLNGD"
     gene            5798..6223
                     /gene="tsaE"
                     /locus_tag="P5123_04475"
     CDS             5798..6223
                     /gene="tsaE"
                     /locus_tag="P5123_04475"
                     /inference="COORDINATES: protein motif:HMM:TIGR00150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     ATPase subunit type 1 TsaE"
                     /protein_id="HRX15548.1"
                     /translation="MVIEKISNSEEETGNIAKEVSMKIKPGSVIAFSGDLGCGKTLFT
                     KYFARFLGVEDEITSPTFNLVEEYEGKLKLYHFDLYRIENSSELDLLGFEDYWESDGV
                     SVIEWAERAMDRLPDDKIVIDIEYVNETTRRFRIEYPDN"
     gene            6204..6896
                     /gene="tsaB"
                     /locus_tag="P5123_04480"
     CDS             6204..6896
                     /gene="tsaB"
                     /locus_tag="P5123_04480"
                     /EC_number="2.3.1.234"
                     /inference="COORDINATES: protein motif:HMM:TIGR03725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003725 - double-stranded RNA binding
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     dimerization subunit type 1 TsaB"
                     /protein_id="HRX15549.1"
                     /translation="MNILIIDTSCEHEIIIVDNGKTFGGAFMNTGFVHSLSLFERVDK
                     VLAQSGCVMSDMDYIICGVGPGSFTGVRIAVTTVRTISQLLNIPVVGVKSPLLYAIRS
                     DIELNSNIMVGFDAKKSKIFGALYRKTEQGYLTLIEEGDYFPDYILDRVEPESNLICL
                     GSGSVKYSEKIIEKSQKARILDNLMPDINSVSAYCKDIAEKHSTKDYTTLMPFYARLS
                     DAEALKNSLVKK"
     gene            complement(6898..8070)
                     /locus_tag="P5123_04485"
     CDS             complement(6898..8070)
                     /locus_tag="P5123_04485"
                     /inference="COORDINATES: protein motif:HMM:NF019245.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1577 domain-containing protein"
                     /protein_id="HRX15550.1"
                     /translation="MVNFSERKKRFFEEIESKQEIFSILQDKFSSTPLKIKYDIEGNE
                     LYLSDFSQIELALTVTTTSTYKIGDDKIIICSALLDKYFEVEFEIKKELAPGQFRCLI
                     HSARRATTGRSDIRFKVKPGDLYATNFKVSKHSIDLTMFKLPTSIKVVLDQFAAQNKA
                     KFDFFEIGYFDKKDTLLNTMKKTGNSFYIENLGDTTTYVPVTDEFINYKELMADEINE
                     YITQNKERNFKSILIVPIVYITPNEQSIPFAYIKIMTKDRLLSIDDFLKIKEYSFKLI
                     DRIRDANTQFIDKRQAILDISRGGARLKIDDPELKKYLLKAKGFIFDLVFKLQQPITI
                     YGEIKFTGTDDNKNILLGLSFSGNTSRKNQMQHLYSVLKPMEINYKKKLIEQLKRR"
     gene            complement(8514..8756)
                     /locus_tag="P5123_04490"
     CDS             complement(8514..8756)
                     /locus_tag="P5123_04490"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRX15551.1"
                     /translation="MAAKTSKQLKADEKKQIAKINKLTGDLKKEKANLVKLKQSIKTA
                     VANEKAKEAKLKAAKKAAKKSTKKVSKKSAKKAVKK"
     gene            9401..10828
                     /gene="der"
                     /locus_tag="P5123_04495"
     CDS             9401..10828
                     /gene="der"
                     /locus_tag="P5123_04495"
                     /EC_number="3.6.5.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR03594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTPase Der"
                     /protein_id="HRX15552.1"
                     /translation="MSKKKPLVAIVGRRNVGKSTLFNALINKKMAIVDDHPGLTRDVI
                     SYRVDTETHSFLISDTPGLDLPDNEELSEPILTTAYEALGKADLIVLMFEDPIVQKFD
                     HDLLRIVRKLDKPTVMVVNKMDSEKQLVNMSNFYELGVSDLLPVSALRRSNLDLLLDK
                     ITAFIPANRGEEQIADMRISFVGKPNAGKSTLLNSLLGYGRSVVSDVPGTTRDSINEL
                     INFENKKIEIVDTAGLRKRSKVKESVEFYSLTRTIRAIEHSDVVIHLIDAQSGMTDTD
                     KKISDEIVKANKPVIIAVNKWDTVSKDTNTFKEYTAELKRRFFRIADFPVISISAKEK
                     VRIHKLLSSAVELFEDSHKRIDTGVLNRRLEQMKNSGRLPGYGSDIKVFFATQMNQTP
                     PTFKFFVNDASKFRLDVVRYFQKELQKLLGIKGLPIKIKIEGKPRNIKNVQEAIDIRE
                     KNRRKKGESRTVRIKKGEKRSKKRK"
     gene            complement(10830..11429)
                     /gene="recR"
                     /locus_tag="P5123_04500"
     CDS             complement(10830..11429)
                     /gene="recR"
                     /locus_tag="P5123_04500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombination mediator RecR"
                     /protein_id="HRX15553.1"
                     /translation="MPSPSEYLEALIQELGKLPGIGSKSASRAAFHILEMNETAVNRL
                     LSTITALRNNVRECTICGGISDTDICSICSNTARNRRIICVVEDKKSVLTIEKTGTFN
                     GLYHILGGVISPLDGIGPENIRFAELQHRCESEDIEEVIMATNPTIEGDATVLYCVKL
                     LKPLNIKVMRLARGLPVGADIDFTDIATISKSMSERHEV"
     gene            complement(11429..11752)
                     /locus_tag="P5123_04505"
     CDS             complement(11429..11752)
                     /locus_tag="P5123_04505"
                     /inference="COORDINATES: protein motif:HMM:NF014616.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YbaB/EbfC family nucleoid-associated protein"
                     /protein_id="HRX15554.1"
                     /translation="MLKGLGDLGNIMKLQKEMKRMQKEIKKNETEGSSSDGMIKITVN
                     GEFECRKVTVDSILLQSSNASQVEKNIQEAFTKAVIDNKKLTESKMREITGNMDLGAL
                     GSMFS"
     gene            complement(11752..13326)
                     /gene="dnaX"
                     /locus_tag="P5123_04510"
     CDS             complement(11752..13326)
                     /gene="dnaX"
                     /locus_tag="P5123_04510"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: protein motif:HMM:NF004046.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009360 - DNA polymerase III complex
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit gamma/tau"
                     /protein_id="HRX15555.1"
                     /translation="MSYQVIARRWRPQKFDQLIYQDHITKTLQNSITSGRISHSYIFT
                     GPRGVGKTTTARILAKSLNCIHGPTPEPCGVCENCKEITQGNSFDVIEIDGASNNGVD
                     NIRELRETVHFAPVKSTYKIYIIDEVHMLSTGAFNALLKTLEEPPAHTVFIFATTEIH
                     KVPDTILSRCQKYFFKKISPDAIAAHLSSIVKKEECDVSESALFAIARAATGSMRDAQ
                     SLLEQMISFSSQSLSDRDITFLLGIVPVTSYLSLLDAIASQNPASITEISSDLYTSGI
                     DISRYTGGLKETLRALRFIKHGIIVDESAGFASEDINVMKQTATKFSDEEISRFFSLL
                     ENLSTTLRYTENETLSFEMTLFDMLAVVKNPSLSSILMKLNQEAEKKKSEGQSYELKP
                     VESAPLSLKQAWSHILLQISTKKKYLFELIGLHTHDFNIDSVILTPKQNSNQIKTIDI
                     NDINFLQTELTKLCHKKIGLIVHNTITPPPLPEKQMIKNDSEVAAEDALINQDPTVKK
                     IVKMFHGEIIKQKGEK"
     gene            complement(13342..13426)
                     /locus_tag="P5123_04515"
     tRNA            complement(13342..13426)
                     /locus_tag="P5123_04515"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(13390..13392),aa:Ser,seq:gga)
     gene            complement(13694..14638)
                     /locus_tag="P5123_04520"
     CDS             complement(13694..14638)
                     /locus_tag="P5123_04520"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRX15556.1"
                     /translation="MTKFKILILLTLLAQFVTLSASTEISFSGEAEIRAGVESTKSED
                     NRSRSYGIPARESGLILNNEIASGNLTGLVSLEINTDGITADSASISYNLGIATLTYE
                     AAGIDQRWNDDMLYSGSVDGHAFTVSLFNLGFISIIGLKSEHEESMEDYTSERAFPVV
                     SGGLDGELSMFQLKLGAVYGQNSDDKNWLLFSDTSTQFGDLSLGFGLTYGKNCGIIVD
                     EDDIINGSTLLSLILSSEYAFGALIPGVEGMYAIKSIKDSGDFSAFSINPYMRYEVDA
                     NLAFSLGVIYESQSVVADDKDIDPDSITTVYTSVNFTF"
     gene            complement(14702..>15151)
                     /locus_tag="P5123_04525"
     CDS             complement(14702..>15151)
                     /locus_tag="P5123_04525"
                     /inference="COORDINATES: protein motif:HMM:NF019292.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside recognition domain-containing
                     protein"
                     /protein_id="HRX15557.1"
                     /translation="TSIPENISAIFDGLSDPLGTGLITETDADTIAEEIGAASSTIGI
                     MAEFFSNKQQAYAYLIFILLYLPCVAALAAVIKEAGYIFGILNAAYVTILAWSVATLF
                     YQITAGQSLVYGLLPVLLMGLIYLVLKIISKNNYLNFTNDKNEEENL"
BASE COUNT         4770 a         2246 c         3053 g         5082 t
ORIGIN      
        1 tgttcagact gcagagggat atcttgatca aagtgcttca attttgcagc gtattcgagt
       61 tctggctgtt cagtcgtcaa acggtattta tactgataat gaccgtcagc ttattcaggt
      121 agaagtttct gctcttattg atgaagttga ccggattgct tcacaagcag agtttaacaa
      181 gtttaaactt cttcttggag atttttcaag aaccaatcct cgtgcctcta tgtggtttca
      241 tatgggagca aacatgcatc agagagagcg tgtttacatc ggaacaatga catcagaagg
      301 tttgggtctt actgaaagaa caggtgagtt tcttgcaaat ctttcaactg ctgataaggc
      361 taacagaaca attggtatca tcgatgatgc tcttcataaa gtatctaagc agagagcaga
      421 ccttggagct tattacaatc gtttagagct tacatctaaa ggacttatga ctgcttatga
      481 aaacattcag gcttctgaat cgcgtattcg tgatgcagat atggctgaaa caatggttga
      541 gcttaccaag gatcaggttc ttgtacagag tggtacttcg atgctggctc aagcaaatca
      601 gaagactcaa acagttcttc agcttttacg atagcggatc aggggttata acggcatttt
      661 aatccggtaa cttttgatct gcgaaggaac ttcgatttga tatatggaat gcaatgacta
      721 actttgggct ggtatcaaaa gtgccgtggt atcaggaaac aggttattca ggtattcaaa
      781 cagttatcat tatgtttgta aaaaagagtt tttcattggg agccgtctgg ttacaggcgg
      841 ctctttttta attaataatt atttatggag ttttattgta ttctttaacc gctttacgac
      901 aatattaaaa atgaagttat tgacatgagt aatatagggg taagaataat cgaaataaag
      961 ttaaagaaaa gatccaattt taatgaataa atatttgtga aagcaaataa aactcttttg
     1021 aagataaata ttttgtcttt attttcaatt atgatatatc tgcttaactc tattgagtat
     1081 taggaatagc ttcttcaagt aaaggcaacg ggagatgatt agtgaaacgt ctggtgttta
     1141 cagttgtagc tctgctttta tggattgttt ttttaatggc aatttttttt gatgatattg
     1201 ttctttttgt ctactttatt aaagagagag ataaagtaat atcagatcaa atgcttaatt
     1261 atgaaaacct atatttatct ggtgacgatc atgcaatata taataattcc actattcatg
     1321 tttcatcctc atttactttg tccggagatt ctttgcttga aatttataat tctaaaatag
     1381 taattactcc gttttacgat aatctttttg aaatggatat aaaagataat tcaaggatgg
     1441 ttataagaaa ttgtgaagtg acaacatctg agggatcagt gcaatactgc aaaatatatg
     1501 atcatgctag tgttgaagtc atagatgttc aaaatgatgg acttaactat atcttgtcag
     1561 atagttccga aataaaaata tataagagtg tttcagatat atcaatttct gctggtgcga
     1621 aacctgatat cttaattgac cgctctcctt atatttctct gctttatgtg ttagatagtg
     1681 ccgaaaaggt ttccgatatt ccagcgaatg aatttgttga ggaatataac aatgaattgt
     1741 ttggccatct agagataaat aagagtttta tacaatctgt tggttttatg ataggatctt
     1801 ccgttaatct tactgttaag gattgtaatg ctgtagaatt aatatggaaa aatgaaagtc
     1861 aaaatgaaat attagtagaa ggttttaagc agcagatgta taaagataag tattttgaat
     1921 ttggtgaaag caaagtcttg ttgttaaata gtcgtgttgt gagatcggta attaataatg
     1981 acggtggagg cgagttaatt atcagagata gtgatttgac cacagcttct gtttccgggg
     2041 atggcatatt aagcggtgaa cggttgagag ttgattattt ggtaatttca gatgaatcgg
     2101 ctgctattct ttatgattct gctgtttctg tgttggcaga tatagctgat cgtgcaaatc
     2161 tttttattga aaatagtgag gtttctgatt atactttttc aagaagagta tctgattttt
     2221 atttttatat tgatggcgaa aagagtaata aaagataata tattagattt ttttacagag
     2281 gttgttatgg atattgaata caagtataat gaattaaaag gtattataga aaatatggaa
     2341 tcagttgatg atagtagtat catcatgaaa attgccgatc ggcttcatgg aatttcatat
     2401 tcaccgccat ttctgattga agtgaaacat tttacggaaa tggataaaaa aagcgctatt
     2461 acggcattga attatatatt tgaatttcct gaaagcgaga ttaagaaatt tgctatttcg
     2521 cgagataagt ctgttgccga gataaagata gatgctatga atggttttgt tgccgaatat
     2581 gggctgttgc agcggttaag aatggatgtt cctgaggcgt gggatcagat aaatgagcta
     2641 tatgaagacg attgattaaa aaatcaaaaa agttgttgta ttaattgtgg taatgataat
     2701 tgttatcata agaggtgaaa atggaaagaa aatcccgtca aaaaactgag atcagaaaaa
     2761 ttctgtccca aaacaatttt catcctacta ttgaagagat ccatctgata cttaaaaaac
     2821 gtgttccagg tgtaggtatc gcgactgttt acaggaatat tgataagatg gttagaaacg
     2881 gcgagattat taggttggag gttccggggc agggagcacg ttttgatggt aatactgaaa
     2941 aacattacca ttttcgatgt gaaaagtgta attctgtaga agatgtatgg ttggatatag
     3001 acataaactc atatatcaaa actgtttcgc ctttaaagga atcgattatc agagaccata
     3061 tgattgaatt tacaggtatt tgtgcagaat gtcaaaaatg aatagttagg attaaaacct
     3121 tgatggtgaa aatttaaaat aaattttcaa ccacttgagg tgttatttat tgaataaaaa
     3181 aattagggag aattattatg aaaagattgt atgtttacaa atgtaatgtt tgcggaaatg
     3241 ttgttgaatt aatgagcgtt gggggtggta ctttatcgtg ttgcggtgaa gacatgcatt
     3301 atatggaaga aaaaagtgct gattcttcaa ccgaaaagca tgtacctata atcgaaccta
     3361 tcgatgaagg tgttagagtt actgtaggtt cgacagagca tccaatgact gatgaacatt
     3421 atatccagtg gatcgaggtt ataaatggag actgggttaa tagaaaatat cttgctcccg
     3481 ggcaaaaacc acaggctgaa ttttatgtgc catttagtga tactttggtt gctagagagt
     3541 tttgtaatgt tcacggacat tggaaaaaca agtagttact aaagtaagac aattataaag
     3601 ttcgttaagt tgaaaaaata gttgattact gggaatttgt aatattaaat aaagctggtt
     3661 gcgattgtga ccggcttttc tgttaatatt gtaacaaaaa agttaatcaa tcgattgcat
     3721 ctgaggtttt gtagtaatta aaatatgaag taaatatata acagccaaga tcaggttgta
     3781 aattgaaaaa aacagtttga gtcttgtcga tccgtttatt attgagaata ttgaagaggg
     3841 aatggctact ccggaataga tgctacaaat tatcagaaat attatacgag attttggttt
     3901 tcgcaaatag aggccaaaag cggtaataat aataaaaata aaaaaaatta cggtaattat
     3961 aaatgaagtc agggtgccgt aaagaaatat gagttgcccg gcaattatta ctatatttaa
     4021 tgtcagcata agtactagat agggtgctgc tgattcaaaa atatttttgt gtattttttt
     4081 cataatgatt aagttgaatc ctttaaaaag agtgtaaagc aatatttgaa ttattaacgg
     4141 atttgttcaa aataatttta gtttaaaaat attatgatat gttaccatta ttcatttcta
     4201 cttgattttt tatgaataat tgcttagaat gattgtaata ttgcttaatg atctgtatgg
     4261 aaggaagcca tgttgagatt gtcccttgtc aggcatgcca agtcaagttg gaaaaatgat
     4321 ttgttgtctg atcatgatcg tccgctgaaa acccgaggca ttagtgattc tggaatgatt
     4381 gcccagaaaa tatccgatct aggattaatt ccacctgtta ttttctgttc gacagctttg
     4441 cgatcacaat tgactgttca gcttatgaac gagatttttg ctctttcgga agagcagata
     4501 cgttatgacc aggttcttta tcgggcgtgg gctgattcaa ttttggatta tgttaaaaaa
     4561 gaggcaaaaa atgaccatat tatggtagtt ggacataacc caggtgttca tcaacttgtt
     4621 gagaaattaa cagattcgcg tatcgaaaaa tttcagacgt gcgcgtatgc ttctattgtt
     4681 tttgattgca atagctggag tgatgttcag gatggcagag gaaagcttga tcattacatt
     4741 tacccccgaa tgttttattg acactaactt cttaaatgca tggagcaagt ttgccgtatt
     4801 gaattaatta ccggatggta gttatataaa aattttataa aatacggtta tcctggcgga
     4861 aaaatggaaa atgaagtaat acaaataccc tcttctgaga gcgctatttc tgagcgtctt
     4921 gataaatttt tagcagacaa ctgttcttgt aatttttcaa gaagttacat tttgaaaata
     4981 attaatcaag gccttgtcag ttgtggcggt aatgttctaa aggcaagcta ccgggttaaa
     5041 gctgatgacg tatttgacct tgttttgcca aagttggaaa agctggattt aagcccacag
     5101 gatatacctt tggatgttgt atatcaggat tctgatattg ccgtcattaa taaacctgca
     5161 ggtttaattg ttcatccggg tgcgggtgca ccgaaagata cacttattaa cgcgctttta
     5221 tatcatcttg acggcttatc gtctattggc ggtgtagaac gtccggggat tgttcatcgg
     5281 cttgatcgag atactgcggg attaatggtt attgcaaaaa atgataatgc tcatatttct
     5341 ttatcgtctc aatttgagaa aagaactgta agtaaagttt atacagcatt agtaaaaggt
     5401 aagcctcgta ttgccaattt tataatagaa aataaaatcg agcggcatcc caaatacaga
     5461 caaaaaatga ctgtttcttc gaaggggcga ctggctaaat cagaaataac tgtaaaaaag
     5521 gtgtgggatt gcaaagacgg aacattctca ttgttaaagg ttaggatttt tacaggaaga
     5581 acccatcaga tacgagttca cctctcatca gaggggttgc caatcatagg agattctgtc
     5641 tattctaaaa gctcgcaaaa gtacaggttg ccgtatttat tattggcaag tgttcattta
     5701 gggtttgatc atcctgttag tggtgataga atggagtttg atgctgaatt gccagaacat
     5761 tttttgaaat ttatcgaaaa acttgacagg tcattaaatg gtgattgaaa aaatatcaaa
     5821 tagcgaagaa gaaactggga atatagcaaa agaagtatca atgaaaataa aacctggttc
     5881 tgttatagct tttagtggcg atttgggatg tggaaaaaca ctttttacta aatattttgc
     5941 acgcttctta ggggttgaag atgaaattac ttcaccgact tttaacctag tagaggagta
     6001 tgaaggaaag cttaagctgt atcactttga tctttaccga attgaaaata gctctgaact
     6061 tgatttgttg ggttttgaag actattggga aagtgatggt gtttccgtta ttgagtgggc
     6121 agaacgagcc atggatcgtc ttcctgatga taagatcgtc atagatatag agtatgtgaa
     6181 tgagactaca aggagatttc gtattgaata tcctgataat tgatacttca tgcgagcatg
     6241 aaataataat tgttgataac ggtaaaacct tcggcggtgc atttatgaat accggttttg
     6301 ttcactcgtt atctctgttt gaaagagttg ataaggtttt ggctcaatct ggttgtgtaa
     6361 tgtcggatat ggattatatt atttgcggtg tggggccagg gtcttttacg ggggttagaa
     6421 ttgcggtaac aaccgttagg actatctctc agctattaaa tattccggtg gtcggtgtta
     6481 aatcacctct gctttatgcg attcgttcag atattgaatt gaattctaat attatggtcg
     6541 gttttgatgc aaaaaaatct aaaatttttg gtgctttgta tcgcaaaact gaacaaggat
     6601 acttaacttt aatcgaagag ggtgattact ttccagatta tattttggat cgagttgagc
     6661 cagaaagtaa tttgatttgt ttgggaagtg gaagtgttaa gtattcagaa aagattattg
     6721 aaaaatcaca aaaggctcgt attctggata atctgatgcc ggatataaat agtgtttccg
     6781 cttattgtaa agatatagca gaaaaacatt ctacaaaaga ttatacgaca ttaatgccct
     6841 tttatgccag attgagtgat gccgaagcat tgaaaaatag tctggtaaaa aaataattta
     6901 tctacgtttt agttgttcga ttagtttctt tttatagttt atctccatgg gttttaatac
     6961 ggagtagagg tgttgcatct ggttctttct tgaagtattt cctgaaaaac ttaatcctag
     7021 taaaatgttt ttattatcat cagttccagt gaacttgatc tcgccatata ttgttattgg
     7081 ctgttgaagt ttaaatacca gatcaaaaat aaatccttta gctttaagca aatatttttt
     7141 tagctctggg tcgtcaattt taagacgcgc tccaccccgg cttatatcaa gtattgcctg
     7201 gcgtttatct ataaattgag tatttgcatc acgtattctg tctataagtt taaaagagta
     7261 ctccttaatt tttagaaaat catcaatgga taacagtcgg tcttttgtca ttatttttat
     7321 ataggcaaaa ggaattgact gttcgtttgg agtgatgtaa acaattggta caattaagat
     7381 cgatttaaaa ttacgttctt tgttttgtgt tatatattca ttaatttcat cagccataag
     7441 ttctttatag ttgataaact catcagttac aggaacatat gtcgtggtat cgcccagatt
     7501 ttcgatataa aaagaattac ccgttttttt cattgtattg agaagagtat cttttttatc
     7561 gaaataacct atctcgaaaa agtcaaattt tgctttgttt tgtgccgcaa attgatctag
     7621 taccactttt atgctagtgg gcagtttaaa catggtaagg tcgattgagt gttttgaaac
     7681 cttgaagttt gtggcataca aatcacctgg tttaaccttg aagcgtatat ctgatcgacc
     7741 cgttgtggct cgacgcgcgc tgtgaattag acatcgaaat tgtcccggag caagttcttt
     7801 tttaatttca aactcaactt caaaatactt atcaagaagt gctgagcaaa tgatgatttt
     7861 gtcatcacca attttataag tgcttgttgt agttacggtt aatgccagtt ctatttgaga
     7921 aaaatctgac aaataaagct cattgccttc aatatcgtat tttatcttta agggagtcga
     7981 agaaaattta tcttggagta tagaaaatat ctcttgtttg ctctcaattt cctcaaaaaa
     8041 ccgttttttt ctttctgaaa agtttaccac tgtaagttaa acctctgaca atcaattttt
     8101 cactcgtctg ctttctataa tatagttaaa atatatttca gtattttcaa ctttttttaa
     8161 aatagttgat ataaatagta taagttatat caatttgcta gaataatgtc aggatgagat
     8221 aaataaacca gtaacagttc gcaataatta aaaaaaacaa gtattagagt caagtacaaa
     8281 agtcagttta gcgtctatga taatatctgt taccttagaa tcttagtaaa cattttatta
     8341 aaaagatgat tataacataa ttatagctcc aaaaaatgtg tttatttgaa gttactatat
     8401 attcagtttg tcggtagata aagaggttct ttaaagagat ttaattggac tttgagaaag
     8461 aaagcagaaa agagagtcta tactctcttt tctgttatga aataaataga taattatttc
     8521 tttactgctt ttttagcact tttcttagaa acttttttag tgctcttttt tgctgctttt
     8581 tttgctgcct taagttttgc ttcttttgct ttttcgttag caacagcagt tttaattgat
     8641 tgctttaact taacaaggtt tgctttttcc ttttttaagt caccagttag tttgttgatt
     8701 tttgcaatct gctttttttc atcggcttta agctgttttg atgtctttgc agccatttaa
     8761 caaatcctcc ataagaagtt taaagtgaat gtctgtttgt tagacagaaa atagtaggta
     8821 aagacattcc gataaacagt gaattttggt agtattaatc aattattaaa attaataata
     8881 aatttatcaa ggattatttg tagtaatgaa gattaattaa agataattgg atttaaaagt
     8941 taaacgcttt gatttaatca ttaataagtt gagattgaaa aagttcgaaa aagatatttg
     9001 ctaaagaaaa aatatttttt ttaaaaacaa taattatcag aaaaatgctt ttatctgtaa
     9061 aatatttcac tgatattgtc ggttaacttt ggttttcaat gatcttttaa taaaaaggtt
     9121 gttatttgtt cgctttcaca taatgaccgg tttcaaaaga acgcaaacat gtaataacta
     9181 gttttgtaat tccaaataag gttaaatttg ttaaatgtat gaatataatt ttgaagtttg
     9241 tgaaataatc gtaattgtaa ggtattatag tctttatggt ttataaatta gataatgtat
     9301 ggaaatgttt gagataaaaa gagtaatgta ttggtagcgt agttttaaaa tgattgaaaa
     9361 aattcgaggc catggagtta tggatagtta gaggaaaaat atgtctaaaa agaaaccatt
     9421 agttgcaata gttggtcgaa gaaatgttgg taaatcgacc ttgtttaatg ctcttattaa
     9481 taaaaagatg gcaatagttg atgatcatcc cggtttaaca agggatgtta taagttatcg
     9541 agttgatacc gaaacacatt cttttttaat cagcgatact ccgggtctag atttgccaga
     9601 taatgaagaa ttatcggagc caattttaac tacagcctat gaagcgcttg gaaaggctga
     9661 tttgatagtg cttatgtttg aagatccgat tgttcaaaag tttgatcacg atcttcttag
     9721 aattgttcgt aagctagata agcctacagt aatggtcgtt aataaaatgg attctgaaaa
     9781 acaattggtt aatatgtcca acttttatga gcttggggta tcggacttgt tgccggtttc
     9841 tgcattgcgt agaagcaatc tggatcttct tttagataaa attactgctt ttattcccgc
     9901 aaatcgcggt gaagaacaaa tcgcagatat gcgaatatcg tttgtaggga aacccaatgc
     9961 gggtaagtct acacttctta attcacttct tgggtatggt aggtcagttg tgtcagacgt
    10021 tccaggcact actcgtgatt caattaacga gttgattaac tttgaaaata aaaaaataga
    10081 aatagttgat actgccggtt tgcgtaagcg ctcaaaagta aaagagagtg ttgaatttta
    10141 ctcgctgaca agaacaatac gggcaattga acattcggat gttgtaatac atttaattga
    10201 tgctcaaagc ggtatgactg atacagataa aaaaatatct gatgaaatag taaaggctaa
    10261 caaaccggta ataattgctg tcaataaatg ggatactgtt agcaaggata ctaacacatt
    10321 taaagaatac accgctgagc ttaaaaggag attctttcgc atagccgact tccctgtaat
    10381 ctctatttct gctaaagaga aagtcagaat acacaaactg ttaagttcgg ctgtggaact
    10441 ttttgaagat tctcacaagc gaattgatac gggcgtatta aaccgcagac ttgagcaaat
    10501 gaaaaatagt ggacgtcttc caggatatgg atcagatatc aaagtttttt tcgcgacaca
    10561 gatgaaccaa acaccgccta cgtttaagtt ttttgtaaac gatgcttcaa agttccgatt
    10621 ggacgttgtg cgctattttc aaaaagaact tcaaaaactg ttaggaataa aaggtttgcc
    10681 gattaaaatt aaaatagaag gtaagcctag aaatataaaa aatgttcagg aagctataga
    10741 catacgtgaa aaaaaccgac gaaagaaggg ggaaagccgt acagttagaa ttaaaaaagg
    10801 tgaaaaacgg tctaaaaaaa gaaagtgaat cagacttcgt ggcgttctga catggacttg
    10861 ctgatagtgg ctatgtcagt aaagtcaata tcagcaccga caggcaatcc ccttgcaaga
    10921 cgcatgacct tgatgtttag tggtttaaga agtttaacac agtagagaac tgtggcgtcg
    10981 ccttctattg tcgggttagt agccattatt acttcttcaa tatcttcaga ttcacatcta
    11041 tgttgaagct cggcaaatct tatattttca gggccgattc cgtccagtgg tgaaataacc
    11101 cctcccagaa tatgataaag tccgttaaaa gttcccgttt tctcaattgt aagaaccgat
    11161 tttttgtctt caacaacgca aattattctg cggtttcttg ccgtattgct gcatattgaa
    11221 cagatatctg tatcggaaat gccgccgcat attgtgcact ctctaacatt gttacggaga
    11281 gctgttatgg tacttaacaa tcggttaacc gctgtttcat tcatttctaa aatatgaaac
    11341 gcagcccggc ttgcgctttt agaccctatg ccgggcagtt tgccaagttc ttgaataagg
    11401 gcttcaaggt attctgatgg agaaggcatt atgagaacat gcttccaaga gcaccgagat
    11461 ccatgttgcc ggttatttca cgcatttttg actcggtcag ttttttatta tcgattactg
    11521 ctttggtaaa ggcttcttgt atgttttttt cgacctgtga cgcatttgaa gattgtagaa
    11581 gtatgctatc aacagtaact ttgcggcact caaattcacc gttaacagta attttaatca
    11641 ttccgtcact gcttgagcct tcagtttcgt ttttctttat ctctttttgc atgcgcttca
    11701 tctctttttg aagcttcatg atattgccaa gatcgccaag tccttttaac attatttttc
    11761 tcctttttgc tttataattt cgccatgaaa cattttaact atttttttaa cagtagggtc
    11821 ctgattgatt aaagcgtctt ccgctgctac ttcggaatca ttttttatca tctgtttttc
    11881 cggcagtgga ggaggggtga tagtattatg tacaataagc ccaatctttt tgtggcaaag
    11941 tttagtaagt tctgtttgta aaaaattaat gtcgtttatg tcaattgttt taatctggtt
    12001 tgaattctgt tttggggtga gtataacgct atcaatatta aaatcgtgtg tatgaagacc
    12061 aataagttca aaaagatatt tttttttggt cgaaatctgt aataaaatat gagaccatgc
    12121 ctgttttaaa gaaagaggag cagattcaac aggttttagt tcataagatt ggccttcaga
    12181 tttttttttt tcagcttcct gattaagttt cattaaaata gaacttaatg agggattttt
    12241 gactacggca agcatgtcga aaagcgtcat ttcaaatgaa agagtttcat tctccgtgta
    12301 gcgaagagta gtggatagat tttcaagcaa agaaaaaaaa cggctaatct cttcgtcaga
    12361 aaatttggtt gctgtctgct tcataacatt tatatcttct gaagcaaaac cggcagattc
    12421 gtcaactatg atgccatgct taataaatcg tagcgcacgt aatgtctctt ttaagccgcc
    12481 tgtatatctt gatatatcaa taccgctggt ataaagatcg gaactaattt cggtaataga
    12541 ggctgggttt tgtgaagcaa tggcatcaag aagcgatagg taacttgtta caggcacaat
    12601 accaagcaga aaagtgatgt ctctatctga tagtgattgt gaagagaatg agatcatctg
    12661 ttccagaaga gattgtgcgt cacgcatgga gccagtggct gctcgggcaa tcgcgaaaag
    12721 agccgattcg gaaacatcgc attcttcttt tttaactatt gaagagaggt gagctgcaat
    12781 tgcatctgga gatatttttt taaaaaagta tttttgacaa cgtgatagta tggtgtctgg
    12841 aactttgtgt atttcagtgg tggcaaaaat aaaaacagtg tgcgcaggag gctcttcgag
    12901 ggttttgagt agtgcgttaa aagcaccggt cgagagcata tgtacctcat caattatgta
    12961 aatcttatag gtcgatttga cgggcgcgaa gtgtacggtt tcgcgcagtt cacggatatt
    13021 atcaacaccg ttatttgatg ctccatctat ttcaattaca tcaaaagagt taccttgtgt
    13081 gatttctttg caattttcac aaacaccgca aggttcagga gtggggccgt gaatacagtt
    13141 aagcgatttg gcaagaattc ttgcagtagt agtcttgccg actcctcgtg ggccggtaaa
    13201 tatatatgaa tgggatatgc gaccgcttgt gatactgttt tgcagggttt tggtgatatg
    13261 atcctgatat atcagttgat caaatttttg cggtcgccat cttctggcta ttacctgata
    13321 tgacattctg atttaccgtt tcggagaggg agggattcga accctcgata gctttcacta
    13381 tacacgcgtt ccaggcgtgc tccttcgacc gctcggacac ctctcctgat aaagtgactt
    13441 tagtcgagtt ttttgaatta tctgaaaaac ccgaatcagt aacaaataca atatatcgtc
    13501 tctgattgtc aagtatcaaa aaaccgagaa gatgtttctg tttagggatt tcgaggttgt
    13561 taagagatta agttatcatg tatataaata tagcaagtat tgtttaaatt gagagtacag
    13621 tttctgtatg aatgtataaa caaaaactgt actctacatt ctcgtaaata tttagaaaat
    13681 gcttgtggtt ttattagaaa gtgaaattga ctgaggtata aactgtggta atactatcag
    13741 ggtctatatc cttgtcatca gcaacaacag attgagactc ataaattaca cctaatgaaa
    13801 atgccaggtt cgcatcaact tcataacgca tatacgggtt gatgctgaat gcggaaaagt
    13861 cgccgctatc ttttatggat tttatggcat acataccttc aacacctggt ataagagcgc
    13921 caaaagcgta ttcgctagac aatataagtg ataaaagtgt ggagccattg atgatgtcgt
    13981 cttcatcaac gatgattcca cagttttttc cataagtaag accaaaacct aatgatagat
    14041 cgccaaattg ggtgctggta tcagaaaata gtagccaatt tttatcatca ctattttgac
    14101 cataaacagc accaagttta agttgaaaca tggataactc accatcaaga ccgccagaaa
    14161 cgactggaaa agcgcgttct gaagtataat cctccataga ttcttcgtgt tcagatttta
    14221 acccgataat tgaaataaaa cctagattga aaagagatac agtaaaagca tgcccatcca
    14281 cgctaccgct atatagcatg tcatcgttcc atctctggtc aataccggct gcttcataag
    14341 taagggtggc aattccaaga ttgtaagaaa tagacgcgct atcggctgtt attccatctg
    14401 tgtttatctc gagtgaaaca agacctgtta ggttaccact ggcgatttcg ttattaagga
    14461 tgaggccgct ttcacgagca ggtataccat aacttcgtga tctgttatct tccgattttg
    14521 ttgattctac tcctgcacgg atttcggctt ctccagaaaa agatatttct gtagatgccg
    14581 aaagagttac aaattgtgct aataatgtaa gtaagattaa aattttaaat tttgtcattg
    14641 taaatcccct attgatattt atttgtaagt agtctttatg gtaatttttc gaatttccct
    14701 tttataggtt ttcctcttca ttcttatcgt ttgtgaagtt taaatagttg tttttagaaa
    14761 ttattttgag aacaagatat attaacccca taagtaatac tggaagcaaa ccgtatacta
    14821 gtgattgacc ggccgtaatt tgataaaata gtgtagcaac cgaccatgcg agtattgtta
    14881 catatgctgc gtttagaatt ccgaaaatgt aacctgcctc tttaattact gctgccaaag
    14941 ccgctacaca cggtaggtat agtaatatga aaataaggta agcgtatgct tgttgcttat
    15001 ttgaaaagaa ttctgccata atgccaatag ttgaagaagc ggctccaatt tcttcggcaa
    15061 tagtgtccgc gtctgtttcg gtgatgagtc cagttccaag tggatcggat aatccatcga
    15121 atatggctga aatattttcc ggtattgaag t
//
LOCUS       DAOTGZ010000043        15046 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_3064
            NODE_4776_length_15046_cov_4.361417, whole genome shotgun sequence.
ACCESSION   DAOTGZ010000043 DAOTGZ010000000
VERSION     DAOTGZ010000043.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868088
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 15046)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 15046)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:22:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,785
            CDSs (total)                      :: 2,751
            Genes (coding)                    :: 2,732
            CDSs (with protein)               :: 2,732
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 12 of 19
            Pseudo Genes (internal stop)      :: 5 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15046
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4776_length_15046_cov_4.361417"
                     /isolate="AMR_MDS_3064"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..454
                     /locus_tag="P5123_04530"
     CDS             <1..454
                     /locus_tag="P5123_04530"
                     /inference="COORDINATES: protein motif:HMM:NF024915.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HRX15558.1"
                     /translation="RSIDRNSGEYPAALYLQGEIYLSLKQSSEAKNSFFQATSLFPGH
                     DLADNSLLQLSRIYLAEGNGEEALKIAVKLLSSYPDRDTSDDAFYMIGKIYEKDSRLK
                     DIERARTIYTNFIKRAEVERRAPYHHSPLLRKVKRDLDFINKNYFVVH"
     gene            complement(459..1574)
                     /gene="hemW"
                     /locus_tag="P5123_04535"
     CDS             complement(459..1574)
                     /gene="hemW"
                     /locus_tag="P5123_04535"
                     /inference="COORDINATES: protein motif:HMM:TIGR00539.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051989 - coproporphyrinogen dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM family heme chaperone HemW"
                     /protein_id="HRX15559.1"
                     /translation="MTDFGIYLHIPFCVKKCSYCAFYSLPKSEKTLFDSYTDALCSEI
                     NSDNYIDSSAVCSSVYFGGGTPSLLSDDNLINILESINNRYSLTNDCEITLECNPAQY
                     EFKRFSRLVQNGINRFSMGIQTLNPQLHDYIGRTGKPVSCDLIESYLSIPALCGVDFI
                     IGIRDGYSALPDIERVIQYKPDHVSAYLLTLESDTALFKYADTKCLDTFQRKEYLDVS
                     NFLKENGYDHYEISNFAINGAYSKHNLKYWNGSDYRGYGAGAHSYLRGERYFHDDDVY
                     LYIDEKGANLRKDNRKPSDILVELVFTGLRTSKGVSEFNYNRITGKKFPEELCKRVQG
                     LSELECVVDNGWRRICIKPVNFVDYDTIIYQLLEPFI"
     gene            complement(1567..3219)
                     /gene="nagZ"
                     /locus_tag="P5123_04540"
     CDS             complement(1567..3219)
                     /gene="nagZ"
                     /locus_tag="P5123_04540"
                     /EC_number="3.2.1.52"
                     /inference="COORDINATES: protein motif:HMM:NF013126.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-N-acetylhexosaminidase"
                     /protein_id="HRX15560.1"
                     /translation="MKSRKIMFVTVISLFLMITLTIAIGEEADNSQKNVQNIVKGMSI
                     DEKIGQLLMIGVPGTSLNKSSKELISTYRPGGLILFGYNYRTTGQLYEFNRSLQNYSV
                     SQKSLPMLISIDQEGGRVRRITGQGVTQFPGNMAAGSANDPLLTYRWARILAMQLRRL
                     GVNMNLAPDIDVNNNPDNPVINTRSFGSNPEIVARHGRAYVKGLQEGGVMAVAKHFPG
                     HGDTSVDSHKTLPVIPYNIKRINSVELVPFKESVAAGVSSVMTAHILYPEIDKNGLPA
                     TLSSVFLNKILREYMKFDGLVMTDDMEMGAIAGNMDIGTAAVVAINAGADIILVTTHH
                     NTKNIYNGFIEAYKKGLLTEERINKSVERIVRAKLKYGIVNPQKTYSYINSYSSESDY
                     REMVSAEKLNNLISKKALYFYRGVPERDYTYSSKAHLFTNNSFIKKQFAVTNAVKIYS
                     ISQLFIKLREIPAEETVYVQLGDSQASIAHRLKTFSANNNNIVILSTENPFEITKYSG
                     TIPVLFSFSNTQESIKAVCDALKGKIDVKDYISIDLGFGRSD"
     gene            3406..3984
                     /locus_tag="P5123_04545"
     CDS             3406..3984
                     /locus_tag="P5123_04545"
                     /inference="COORDINATES: protein motif:HMM:NF024970.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HRX15561.1"
                     /translation="MAEQHKEIHFLDSFKVDTRIIREVIDNLMIDLNRCDYVKEEIDE
                     IILAMDEAITNAIQETLFSKKECESESKLTHEITIRYRVTYEEFDATVIDHGKGLDID
                     KMLKNTPNKESADYKQQLFDYAKKSESSKLKIRLNGKEISLNGIGAGLRIILSFMDKL
                     NIDLIDKKSVLSTNVCSSTEGSILNMQRKRRY"
     gene            3984..4736
                     /locus_tag="P5123_04550"
     CDS             3984..4736
                     /locus_tag="P5123_04550"
                     /EC_number="3.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020259784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016799 - hydrolase activity, hydrolyzing
                     N-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0009691 - cytokinin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LOG family protein"
                     /protein_id="HRX15562.1"
                     /translation="MKNVKAPLSYLDPDFVQSKEGRPLRILSEYLDPERKLKLNNIEH
                     TVVFFGSARTKPDSSDPLSFAYNAAEDLAYNLAKWSSTIEDPSRKIYICTGGGPGAME
                     AANKGAARAGEKTIGLNIALPFEQEPNPYISPELNMEFHYFYMRKLWFLYHSKAIIVF
                     PGGFGTMDELFETLTLIQTKKIDKPHLPVLLYNKKFWESLINFDIMKDNGLISPEDLN
                     LFRYFDTTDEAMKILVPEITELVDNYSAKNFF"
     gene            4747..5535
                     /locus_tag="P5123_04555"
     CDS             4747..5535
                     /locus_tag="P5123_04555"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRX15563.1"
                     /translation="MRLSTAALLCTLLLSCASSPRGSLSDKSTFPDRYGAPSDSVSAK
                     DGTEDSALIDADDSSLPEAVFDPGAPEEDDSGKSSILPSPEKTVPDKPDDSIKDRAFL
                     DSIKVAPLSKIPKEYRLYTNNRFNYQLFAPQNWYINESTVSDTAVTRISRNSSVMSIK
                     TYKPYRLKKDYYKLKAVREIKLKDAKYKTIIAGEELRLYSGVFAILMVHEYKYRNVRY
                     LSRTLTFIQEGNAYIIRCEAPISRFYKDEKHFNTLMTSFRYLRE"
     gene            complement(5621..5706)
                     /locus_tag="P5123_04560"
     tRNA            complement(5621..5706)
                     /locus_tag="P5123_04560"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(5669..5671),aa:Leu,seq:taa)
     gene            complement(5712..5783)
                     /locus_tag="P5123_04565"
     tRNA            complement(5712..5783)
                     /locus_tag="P5123_04565"
                     /product="tRNA-Cys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(5749..5751),aa:Cys,seq:gca)
     gene            complement(5932..6495)
                     /locus_tag="P5123_04570"
     CDS             complement(5932..6495)
                     /locus_tag="P5123_04570"
                     /inference="COORDINATES: protein motif:HMM:NF014707.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="epoxyqueuosine reductase QueH"
                     /protein_id="HRX15564.1"
                     /translation="MSSKLLLYTCCAPCLSGVFSQLNSYDVVSYYYNPNIADKNEHDS
                     RLQELIRYADMENISIIHELFNRDDLISWTNLVGPYKDLGEKSYRCELCFQFRLEKTF
                     KTAKKMGITTVASTLSVSPHKNAELINQIGNELAQKYSLNYLESNFKKNGGFALSVQN
                     SKKYGFYRQNYCGCAYSKKESLMRTKN"
     gene            complement(6554..6865)
                     /locus_tag="P5123_04575"
     CDS             complement(6554..6865)
                     /locus_tag="P5123_04575"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRX15565.1"
                     /translation="MNMPVSEIIKEQDVQSACRGEIKECEKHEIISSISQIISDCPSS
                     IAKAEQIFDLVKYSVAQYVVKYEMESVYYSNQDLQFMLEEKTSPESVDALCSIIEQFQ
                     L"
     gene            complement(6985..7968)
                     /locus_tag="P5123_04580"
     CDS             complement(6985..7968)
                     /locus_tag="P5123_04580"
                     /inference="COORDINATES: protein motif:HMM:NF012981.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016301 - kinase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase family protein"
                     /protein_id="HRX15566.1"
                     /translation="MPFSPYERKKPVIGVIVNPYSRRNLKTKCAAVEQFASIGNGKAD
                     VRATSSLEELDRILMEFKEKNYLYIGISGGDGTIHNVITRLIHIYRPDPIPAILLLND
                     GTMNNIAISIGLKGDSTQILSSFFSMVDNTFTPRMVTRDTMKVGDRYCFLFGFGMVSN
                     FLNECYSDGKKGFRKNIMAIGKTVSETIGDVLNNLTLKKESELSVMKSMNLSVKVDSQ
                     QVGFRNVLFVLAGTVEQVGMGFKTLYRANDMPRKFHCLINGMRPIDIVTQLSRFAAGV
                     RVENSLNIDETISLLEVEADSPFEFTMDGDMYVSDGSLEVESGIPVRFILV"
     gene            8220..8294
                     /locus_tag="P5123_04585"
     tRNA            8220..8294
                     /locus_tag="P5123_04585"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:8255..8257,aa:Pro,seq:ggg)
     gene            8309..9121
                     /locus_tag="P5123_04590"
     CDS             8309..9121
                     /locus_tag="P5123_04590"
                     /inference="COORDINATES: protein motif:HMM:NF012579.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HRX15567.1"
                     /translation="MHFKHLTKDSIIFSDKTEKQHIIREMVSKLEEAGKIKNSMRYYA
                     QVIHRESLENTGIGNSFAIPHARTESVDELSMLFCVAKNGIDYDSYDGKPVRFLLLSI
                     FPSSYSTTYLYYISMMATIFSDDSIISRLENAQNADEVYTILEEQSGFYFDNISEQER
                     DEVDHSANLAGVPSAELDLLIRLDRLYKMNGSMQCESIMEKITNLEHLIDNRSLTYYK
                     RMQQKCATPFAFVEKQACSGCHMNIPPIELNAIKERKNISLCSNCGRFLLYV"
     gene            9435..11000
                     /locus_tag="P5123_04595"
     CDS             9435..11000
                     /locus_tag="P5123_04595"
                     /inference="COORDINATES: protein motif:HMM:NF012245.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein"
                     /protein_id="HRX15568.1"
                     /translation="MTKGFFVKLDSVLLKRYSNRSLLDQQRASLFIKALLIFSIITFL
                     SALNTIITSPDNATLFYKISMGMIIGGYFISLLLLKAGYYYTSVLITIFLPLTNLLFQ
                     GLSVQSAAGKYIYSMYFYIFLVIAALFGNYKTILITTAGVVSFGATVIINSGDLIPSE
                     SYAIIIMSIFFISVFISVLCILIFRIVTSSIKQINDQNKILEGNVSRLANVIETIKDI
                     SKTLVANSEEISAYSATFSGNAQTQAASIEEITSTLEEVQASAESSATMTESQVKKTE
                     DLIKHLQEMYTLISENKENLDQGLGQKKSLDSSIQIAIREVQNCQKSMDSVLSSISKV
                     NDSTALINDISDQINLLSLNASIEAARAGDQGKGFAVVADEVGKLAENTQENAREITE
                     LVIITSREMQSTRESLDRVIKSSNDVVDYASDLGRVIIDVNTVSETDLKINSDLQRNA
                     SSVLSGSDEINKSMEELKNAIMEITSSISVINDSTQQLAAGAEEINATAQALSESEIK
                     MRNLMNSENSDSE"
     gene            complement(11045..11611)
                     /locus_tag="P5123_04600"
     CDS             complement(11045..11611)
                     /locus_tag="P5123_04600"
                     /EC_number="3.2.2.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003867359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008725 - DNA-3-methyladenine glycosylase
                     activity [Evidence IEA];
                     GO_process: GO:0006284 - base-excision repair [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-3-methyladenine glycosylase I"
                     /protein_id="HRX15569.1"
                     /translation="MNRCDWVNMKNPLYIKYHDEEWGVPVHDDRKLFEMLILEGVQAG
                     LTWETVLNRREAYREAYDNFEPSIVANYSEKKMENLLSNSSLIRNRRKISASVKNASV
                     FLDIQFQYGSFSDYLWDYVSGKPIQNGFSNISEVPASTPLSEKFSVDLKKRGMSFVGP
                     VIIYSFMQAVGLVNDHIKNCFRYSQLTK"
     gene            complement(11679..12956)
                     /locus_tag="P5123_04605"
     CDS             complement(11679..12956)
                     /locus_tag="P5123_04605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009622177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HRX15570.1"
                     /translation="MKKNITGISKTIFLLQIKIKVLFHYTKELIQGKITFIKFVRLLR
                     RLLYLIKKLSHNKFVRINGETKIDLYIPGFGSKAFWTSCNKFKEFEKSLPCSTVLISV
                     TNACKNRCSHCYQRFDKGEDISIDKLTNVVKQLQDMGIAFFNIEGGDPFIKFDRLKVV
                     CEQIDDRSSIWINSSGDGITEERLHILKEYPVKAIMFPLTYTDKDIYDKFFKRKGAFN
                     TMIKAIEMCHKNKVPVAINTCPPATNLHNGEFDRVMKMAKDLGAAMVQVIKLKEAGAA
                     LNSDIVDYSKEDLEILKNKVNEYNNGSRYKDYPSISAQIIEESKEMFGCTAGGTERFY
                     INAKGDVQPCEFLNISFGNIADQPFVQIYNSMKSVFNDGRDCWLCEKYNMEIFALFKK
                     TKILPLSADKSKEIYQDWDRGNKTNLYKQIIRM"
     gene            complement(12953..13981)
                     /locus_tag="P5123_04610"
     CDS             complement(12953..13981)
                     /locus_tag="P5123_04610"
                     /inference="COORDINATES: protein motif:HMM:NF027681.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HRX15571.1"
                     /translation="MNKVFITGATGFVGPHIVEFFTKNKVPVTCMVRETSDTSSIADL
                     PVTIVYGDIRDKDRLSALMKGHKCVIHNAAHVSDWGDWQSFEDINISGTCNVLEAAAE
                     NRIDHIIMTGSISVYGEENSYTVKDEHSPLNSHYNYFLKALFPCKMNYYRDSKKIALV
                     EAQKLAVQYKINLTAIHPGWIFGEGEYNTIFYETIKLIKETFPVAMYGKNSLFITYAQ
                     DIASIMYNVYEKKITGVNNYLVGYSPSIKIKDIIELYCKYVGLKSPVRIPLCIISPVA
                     FIIELIYTLLKIKNPPLLTRGRVKTFYDNLEFSTEKIDKELGFIPQYSVEEAIKRTTD
                     WYKNMGIL"
     gene            complement(13974..14978)
                     /locus_tag="P5123_04615"
     CDS             complement(13974..14978)
                     /locus_tag="P5123_04615"
                     /inference="COORDINATES: protein motif:HMM:NF014788.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AIR synthase related protein"
                     /protein_id="HRX15572.1"
                     /translation="MNIEKNIIEKIRKLYPYDKNQINRCFESDSEIIKDNDSYLLCTI
                     DEFSKDDYFGESDPALLGKNLASASLTDIYVSGGDPLWIMHSLTASSKWSEKYIVNLN
                     KAMSGVYKEHNVYMIGGDFSIDCDWRFTSSVIGKTVKPIMRSGANHGDIIFISRKIGG
                     CNVNAAVQSLKLPLLNKVTSFRVELDYKQIKIMQKFASSCTDTSDGFLHAIKSLCDAG
                     DHGCELTDIPLVSGSNIIEKLSGYSRYLLAFAEAGEYAFCFTVPKSRYDEFINEARSE
                     KVKFYEVGAVVEQKTKRITNEHFTIDFTGYSFSARDYKNMKDYIEMLNKWFQKSCSYE
                     "
BASE COUNT         4748 a         2990 c         2519 g         4789 t
ORIGIN      
        1 cagatcaatt gaccgtaatt caggagagta ccccgcggca ctttatcttc agggtgaaat
       61 atatttatca ctaaaacaga gttcagaagc aaaaaactca ttttttcagg ctacttccct
      121 ttttccaggc cacgatctcg cagacaactc attgcttcag ctaagccgaa tatatctagc
      181 ggaaggaaac ggtgaagaag ctttgaaaat tgccgtaaag cttctgtcaa gctatcccga
      241 tcgcgacact tctgatgacg ctttttatat gattgggaaa atttacgaaa aagacagcag
      301 attaaaagac atagagagag caagaacaat atataccaat ttcatcaaaa gagctgaagt
      361 tgaaagaaga gcaccatatc accactcacc actgcttaga aaagttaaac gtgacttgga
      421 ttttattaat aagaactatt ttgtggtaca ttaaaaatct atataaaggg ctccagtagc
      481 tgatatatga ttgtgtcgta atcaacaaaa tttactggtt taatacagat tcttctccat
      541 ccattatcaa ctacacattc cagttctgat aatccttgaa cccttttgca taattcttca
      601 ggaaattttt taccggttat tctgttatag ttaaactccg atacgccttt cgaggttctt
      661 aaccctgtaa acacaagttc tacaagaata tcactaggtt ttcgattgtc ttttctaagg
      721 tttgcaccct tttcatcaat atacaagtag acatcatcgt catgaaaata tctctctccc
      781 cgaagataag agtgagcgcc cgctccatac cctcggtaat cggagccatt ccaatatttt
      841 agattatgtt ttgaataagc accgttaatc gcaaaatttg aaatctcgta atggtcgtaa
      901 ccgttctctt ttagaaaatt tgagacatca agatactcct ttcgctgaaa agtgtccaaa
      961 cacttagtat ccgcatattt aaaaagagca gtatcagact ctagggttaa aagataagct
     1021 gaaacatgat ccggtttgta ttgtatcacc ctctctatgt ccggaagtgc tgaatagccg
     1081 tcacgtatgc caattataaa atccactcca caaagtgccg gaatagataa atagctttca
     1141 ataagatcac aagatacagg ttttcctgtt cgcccaatat aatcgtgaag ttgtgggttt
     1201 aaagtttgga tgcccattga aaagcgatta attccgtttt gaacaagacg gctaaatctt
     1261 ttaaattcgt actgagccgg attacattct aaagtaatct cacagtcatt agtcaaactg
     1321 taacgattat ttatagactc gaggatattt atgagattat catcagataa gagcgaaggt
     1381 gttccacctc cgaaatagac agatgaacaa acagcggatg aatcaatata gttgtcacta
     1441 ttaatttcgc tacaaagggc atctgtataa ctgtcgaaaa gagtcttttc actttttggt
     1501 aatgaataaa aagcacagta cgaacacttt tttacacaaa acggtatatg aagataaata
     1561 ccgaaatcag tcacttcgac caaaacccaa atctatggaa atataatctt ttacatctat
     1621 cttacccttt aatgcatcac aaaccgcctt aattgactct tgcgtatttg aaaaagaaaa
     1681 tagaacagga atcgtaccac tgtatttggt tatttcaaaa ggattttctg tagataaaat
     1741 tacaatattg ttattgtttg cagaaaaggt ctttaacctg tgggcgattg aagcctgtga
     1801 atcaccaagt tgtacataaa cagtttcctc ggcaggaatc tcacgcagtt ttataaacag
     1861 ctgagaaata gaataaatct taaccgcatt ggtcacagca aactgttttt taataaaact
     1921 gttatttgta aacagatgag ccttactact gtaagtataa tctctctcgg gaacaccacg
     1981 ataaaaatag agagcctttt tagagatcag attattgagt ttctctgctg ataccatctc
     2041 cctgtaatcg ctctcggagc tgtatgaatt tatatacgaa taggtttttt gtgggttaac
     2101 aataccgtat tttaacttgg ccctgacaat acgttcgacc gatttattga ttctttcttc
     2161 ggttagcagc ccttttttgt aagcttcaat aaaaccgtta taaatattct ttgtattgtg
     2221 gtgagttgta acaagaataa tatcagcacc ggcatttatg gcaacaacag ccgctgttcc
     2281 gatgtccata ttacccgcta tcgcgcccat ctccatatca tcagtcatta ccagaccgtc
     2341 aaatttcata tactctctta atatcttgtt caaaaaaacc gaggaaagtg tagcaggtag
     2401 cccattctta tcgatttcgg gatagagaat atgcgcagtc atcaccgatg aaacccccgc
     2461 agcaaccgat tctttaaacg gcacaagttc aacactgttt atacgcttta tattgtatgg
     2521 aataacgggc aaagttttat gcgaatcaac actggtatcg ccatgacctg gaaaatgttt
     2581 agcgacagcc atcacaccac cctcttgtaa cccttttaca tatgccctac cgtgacgtgc
     2641 gactatttcc ggattcgaac caaaagagcg cgtatttata accggattat ccggattatt
     2701 attaacatca atatcaggag ccaaattcat attcactccc aaccgcctaa gctgcatcgc
     2761 caatatacgt gcccatcgat aagtcaaaag cggatcattt gcagagcctg cagccatgtt
     2821 accaggaaac tgtgttactc cctgtccggt tatacgccgt actcgccctc cctcctgatc
     2881 aatcgatatt aacataggca aagacttttg acttaccgaa tagttttgta aagatctgtt
     2941 aaactcatat agctgccctg ttgttctgta attatagcca aaaagaatca gaccgcctgg
     3001 tctgtatgta gagattagtt ctttagaaga tttgttaaga gaagttcctg gtaccccgat
     3061 cattaaaagc tgaccgattt tctcatcgat actcatgcct ttgactatat tttgtacatt
     3121 tttttgacta ttatcagctt cttctccaat tgcaattgtc aaagtgatca ttaaaaaaag
     3181 cgatattaca gttacaaaca tgatttttct tgatttcaat acagtatatc ccgatatttg
     3241 aagtaccggc ttaaataaat agaagtatat aagcactaag cttcttcttt tatctgccgt
     3301 tctgtcttta ttattctatg atattttgct agcaaacagt ttttttaatt gataaataaa
     3361 atattagata gactatgcaa aatgataacg atttttgagg attttatggc tgaacaacat
     3421 aaagaaatac attttcttga cagttttaag gttgatacac gcattatacg cgaagtcata
     3481 gataatctta tgattgacct gaatcgttgc gactatgtca aagaagaaat tgacgagatc
     3541 attttggcca tggatgaggc aatcaccaac gctatacagg agactctttt ctccaagaag
     3601 gagtgcgagt ctgaatccaa actgacccac gaaattacaa tacgttatag ggtaacatat
     3661 gaagaatttg atgctacagt tattgatcac gggaaaggtc ttgatattga caaaatgtta
     3721 aaaaatacac cgaacaaaga atccgcggat tataagcagc aactttttga ctatgcgaaa
     3781 aaatctgaat ctagcaaatt aaaaatacgt cttaatggca aagagatatc gttaaacggt
     3841 attggtgccg gcttaagaat aatattatca tttatggata agcttaatat tgatctgatt
     3901 gataaaaaat ctgttttaag taccaatgta tgcagcagta ctgaaggctc tatactaaac
     3961 atgcaacgaa aaaggagata ttaatgaaaa atgtcaaagc tccgctttcc tatctggatc
     4021 cggatttcgt acaatcaaaa gaggggcgcc ccttgcgcat attaagtgaa tacttggatc
     4081 ccgagcgcaa actcaaactt aacaatattg agcacactgt agttttcttt ggatcagcac
     4141 gtactaaacc tgattcatcc gatcctctct cttttgccta caatgctgcc gaagatctgg
     4201 cctataatct tgcaaaatgg tcttccacta tcgaagatcc ctcacgcaag atatatattt
     4261 gtacaggtgg gggcccagga gcaatggaag cagccaacaa gggtgctgcc agagcaggtg
     4321 agaaaaccat cggtctgaat attgcactgc cttttgaaca agaaccaaat ccatatatct
     4381 cgccagagct taacatggaa tttcactatt tttacatgcg caaactctgg tttctttatc
     4441 actcgaaagc aattattgtt ttcccgggcg gctttggcac aatggacgaa ctttttgaaa
     4501 cgttaaccct tattcagaca aaaaaaatag acaaacctca tcttcctgta ctgctttata
     4561 ataaaaaatt ctgggaatct ttgataaatt ttgatattat gaaggataac ggcttgatta
     4621 gtcccgaaga cctgaatcta tttcgctatt ttgacactac tgatgaagcg atgaaaattt
     4681 tagtaccgga gattactgag ctagttgaca actactctgc caaaaacttt ttttaaggag
     4741 tcgataatga gattatcaac ggcagctctt ctgtgtacac ttctattatc ttgtgccagt
     4801 tctcccagag gctcgctttc cgataaatct acatttcccg accggtatgg ggcaccatcg
     4861 gactctgtct ctgcaaaaga tggcaccgaa gactcagcct taattgacgc tgatgattca
     4921 agtttgcctg aagcagtttt tgatccaggt gccccagaag aagatgattc tggcaagagc
     4981 agtattttgc catctcccga aaaaactgta cctgacaaac cagacgatag tatcaaggat
     5041 agagcttttc ttgacagtat aaaagttgcc cccttaagta aaattccgaa agaataccgt
     5101 ttatacacca ataaccgttt caattatcaa ctatttgcac cacaaaactg gtatatcaac
     5161 gaatctactg tctcggatac ggctgttaca cgaatttcga gaaacagctc tgttatgagt
     5221 attaaaacat acaaaccgta ccgccttaaa aaagattatt acaaactcaa ggccgttcgt
     5281 gaaattaaac ttaaagacgc aaaatataag acgataattg ccggtgaaga gctccggctt
     5341 tacagcggtg ttttcgctat tttaatggtt cacgagtata aatacagaaa tgttcgatat
     5401 ttaagccgaa ccctgacttt tattcaggaa ggtaacgcat atattataag atgtgaagct
     5461 ccgatttccc gtttttataa ggacgagaag cacttcaata cgcttatgac aagctttcga
     5521 tatttgcggg aataaaatta ctaaaaactg catggaattt taacaattgc ccaaataaag
     5581 caaaagccgt aagctctgaa caagtcagaa cttacggctt tgcccaagac cggacttgaa
     5641 ccggtacggc cacaagagcc aagggatttt aagtcccttg tgtctaccaa attccaccac
     5701 ttgggcaaat gaggcgtcga gcggattcga accgctgaat aaaggttttg cagacctgcc
     5761 ccttaccact tgggtacgac gcctttacag gacttattta taaacaggtg cctttattgt
     5821 caaacttttt aaacagtagc agagcaaaaa agtaaccact ttacctttct atataacgga
     5881 taaatttata cgagaccatc ttcccttctg ctacgcataa aactattcga attagttttt
     5941 agtacgcatc agggattctt tttttgaata cgcgcaaccg caatagtttt gacgataaaa
     6001 accatatttt ttggaatttt gaacagaaag cgcaaaccca ccgttttttt tgaaatttga
     6061 ttcaagataa tttaatgagt atttttgggc taactcattt ccgatttgat taatcagttc
     6121 ggcattcttg tgaggactga ccgataatgt cgaggccacg gtcgtaattc ccattttttt
     6181 tgcagtcttg aaggtctttt cgagtctgaa ctggaaacag agctcgcatc tgtaagattt
     6241 ttcaccaaga tccttgtatg gacctaccaa atttgtccaa ctaatcagat cgtcccgatt
     6301 aaataattcg tgtataatgg atatattttc catatccgca tacctaatga gttcctgtaa
     6361 tctggaatca tgctcatttt tatccgcgat attggggtta tagtaatagg acacaacatc
     6421 atatgaattt aactgagaaa agaccccgct aagacacggg gcacaacagg tatataaaag
     6481 caacttacta ctcataatgt ttgaaaatgc ctttaaggat cacgtagcta tttataaatg
     6541 cacagaacct attttacagt tgaaactgtt cgataataga acatagtgca tcaaccgatt
     6601 ccgggcttgt tttttcttca agcataaact gtaaatcctg atttgaataa taaacagatt
     6661 ccatctcgta cttgactacg tactgtgcaa cagaatattt aacaaggtca aagatctgct
     6721 cagctttagc aatggacgaa ggacaatcac tgataatctg agaaatagaa gaaataattt
     6781 catgcttctc acactcttta atctcgccac ggcaagcaga ttgtacatcc tgctctttaa
     6841 ttatttcaga aactggcata ttcatggtta atatccttac tttagtttct tagaaaataa
     6901 ggacataaca cgaaagcgtc aacctaaaat aacactttta tgttattttt tttaaatttt
     6961 cgtgtttttt tttataaaaa aatgttaaac cagtataaat ctgactggta ttccgctttc
     7021 gacctcaaga gatccgtctg aaacatacat atcaccatcc attgtaaact caaagggact
     7081 atctgcttca acttcgagca gtgaaatagt ttcgtctatg ttaaggctat tttcgacccg
     7141 aacacctgct gcaaaacggc taagttgagt gacaatatcg attggtcgca ttccattaat
     7201 taggcaatga aattttctcg gcatatcatt agccctgtac aacgttttaa accccattcc
     7261 aacctgctca accgttcctg ctagcacaaa aagtacattt cgaaacccaa cttgctgcga
     7321 atcaactttt actgaaagat tcatggattt cattacggaa agctctgact cctttttcaa
     7381 agtcagatta ttaagcacat cacctattgt ctcggaaacc gtcttgccaa tagccataat
     7441 gttttttctg aaaccttttt ttccgtcact gtagcactca ttcaaaaaat ttgagaccat
     7501 accaaatccg aacaagaagc agtaccggtc ccccaccttc atagtgtctc gagtaaccat
     7561 gcgtggtgta aacgtattgt cgaccatact gaaaaaagag cttaatatct gtgtactatc
     7621 tcctttaagg cctatactga tagctatatt gttcattgtt ccatcattca gaagaagaat
     7681 tgccggtatc ggatccggac gataaatatg tatcaagcgt gttattacat tatgtattgt
     7741 tccgtcccca ccagagattc ctatatagag ataattcttc tctttgaatt ccataagaat
     7801 tcgatccaac tcttcgagtg aagatgtcgc tcgcacatct gcttttccgt taccaataga
     7861 tgcaaactgc tctacagcgg cacattttgt tttaagattt cgcctggaat atggattaac
     7921 gataactccg attacaggct tttttctttc ataaggagaa aaaggcataa cgcgtctcca
     7981 ctatcaggta ttttgaattt tgcaaactag cctcttttaa tgtagttaac agatcaaaca
     8041 agattgggaa ttacacccta ttcaatttac aacagagaga accatattaa ggcaagcaat
     8101 aaataatttg aaaaaagata aactttatta acaatatcta cagttacggt ctaaaatttg
     8161 ctttacaatt atcacacttt tcttaacttt gttaaatcac atgtaacaat gtgtttattc
     8221 ggggtgtagc gcagtccggt tagcgcacat gactgggggt catggggtcg gaggttcaaa
     8281 tcctctcatc ccgacataga ggtaccgcat gcatttcaaa cacttaacta aagactctat
     8341 catcttttca gacaagacag aaaaacagca cataattcgt gaaatggttt ccaagcttga
     8401 agaagctggc aagataaaga actctatgcg ttattacgcg caggttattc accgggagtc
     8461 tcttgaaaat accggcatag gaaattcttt tgcgattcct cacgcgcgta ccgaatcggt
     8521 tgatgagctg tctatgttgt tttgtgtagc aaaaaacgga attgattacg actcttacga
     8581 tggaaaacct gtaagatttt tgctactatc aatttttccg tcatcataca gcactaccta
     8641 tctctattac ataagcatga tggctaccat tttcagtgat gattcaatta tttcccgtct
     8701 ggaaaacgct cagaacgctg atgaggttta tactattctg gaagaacagt ctgggttcta
     8761 cttcgataat atatccgaac aggaacgaga tgaggttgat cacagtgcca atcttgcggg
     8821 tgttccgagc gcagaacttg atcttttaat cagacttgac aggctttaca agatgaacgg
     8881 ttcaatgcag tgtgaaagca ttatggaaaa aattactaac ctcgagcatc ttattgacaa
     8941 ccgatcactt acttattaca aacgcatgca acagaaatgt gctacaccgt ttgcatttgt
     9001 cgaaaaacag gcctgctctg gctgtcatat gaacattcct ccaattgaac tcaatgccat
     9061 caaagagcga aaaaacatat ctctttgttc taactgtggt cgttttttat tatatgttta
     9121 gccaaattag caaatgattt cgattttttc tcttattttt ttataatcat tttctcgttg
     9181 caccgtactc atttgcataa tatcaatatt ttttatcttc cgaactcttt tcattttaca
     9241 ctatcacctt ctaaagataa tttgacttta ctgttatttt tcttatattt aggtagaata
     9301 tgagtgtaat tatccatata tcgatttgat acattttctt aaaaaattgc aggtattctc
     9361 taatgattaa tttatcaatg ggatatacca tcatgaagct ctctgataaa atttttcccg
     9421 ggcggtacca taatatgaca aaaggatttt tcgttaaact tgattcagtt ttgctaaaga
     9481 gatattcaaa ccgctcttta cttgatcaac aaagagcctc gctttttatc aaagccctgc
     9541 ttattttttc gataatcact ttcctgtcag ctttaaatac gataatcaca tcacctgaca
     9601 atgctacact tttttacaag atttcaatgg gtatgattat cggtggatac ttcatttcac
     9661 tacttctgct taaagcaggt tattattata catcagtatt aatcactata ttcttaccat
     9721 taactaatct tctttttcaa ggtttatcgg tgcaatcagc tgcaggaaaa tatatatatt
     9781 ccatgtactt ctacattttt ctggtaattg cggcactatt cggaaattac aagactatac
     9841 taataactac agcaggagtt gtaagttttg gagccactgt tataatcaac tcaggtgacc
     9901 ttattccatc ggaaagctat gccattatta tcatgagtat attttttatt tctgttttca
     9961 tctctgttct ttgtatactt attttcagaa ttgtgacatc ttcaattaaa caaattaacg
    10021 atcagaacaa gattcttgaa ggaaacgtgt cacgtttggc aaatgtaatt gaaactatta
    10081 aagatatatc caaaaccctt gtagctaatt ctgaggaaat atcggcatac tcggctacgt
    10141 ttagtggcaa tgcccagaca caagctgcaa gcatagaaga gattacctca acccttgaag
    10201 aggttcaggc ctccgcggaa tcatccgcaa caatgacaga atctcaggtt aaaaaaaccg
    10261 aagatcttat aaaacatttg caagagatgt acacacttat atctgaaaac aaagaaaatc
    10321 ttgatcaggg attaggtcaa aaaaaatcac ttgatagcag tattcaaatc gctattcggg
    10381 aagttcaaaa ttgtcaaaaa tcgatggaca gtgttctttc gagtattagc aaagttaacg
    10441 attcaacagc attaattaat gatatttcgg atcagataaa cttgctttca cttaacgcct
    10501 caattgaggc agcaagagcc ggagaccaag gaaaaggttt cgcggtggtt gccgatgagg
    10561 tcggaaaact tgccgaaaac actcaggaaa acgcacggga aataacagaa cttgtaataa
    10621 tcacatcaag agagatgcaa tcaacacggg aaagcctgga cagggtaatc aaatcatcaa
    10681 acgacgttgt cgattacgct tcagatctag gaagggtaat cattgatgtg aatacagtat
    10741 cagagacaga cctcaagata aacagcgatt tacaaagaaa tgcatcttct gtattaagcg
    10801 ggtctgatga gattaataaa tcaatggaag agcttaaaaa cgcgataatg gaaattacca
    10861 gctctatatc tgtaataaac gactctacac agcaactagc cgcgggcgcc gaagagataa
    10921 acgctacagc tcaagcactc tcggagagtg agattaaaat gcgaaatctt atgaattctg
    10981 aaaacagtga tagtgaatag ttagacatac atcaataata ttaattatta gagtttatca
    11041 taaactattt agttaactga ctataacgaa agcaattctt aatatgatcg tttacaagac
    11101 caacggcctg cataaaagag tatataatta ccggacctac aaaactcata ccgcgctttt
    11161 ttaaatctac tgaaaatttt tcagaaagag gagtacttgc aggaacctcg ctaatattgc
    11221 tgaatccgtt ttgaattggc ttaccactga cataatccca cagatagtct gaaaaagacc
    11281 catattgaaa ctgtatatcc agaaatacag aggcgttctt gacagaagcc gatatcttac
    11341 gccgattacg aatcaatgag ctgtttgata acagattttc catttttttt tctgaataat
    11401 ttgcgacaat agagggttca aaattatcat aagcctcacg atatgcctca cgtctgttta
    11461 atacagtttc ccatgttaat cccgcctgta caccttcaag aattagcatc tcgaatagct
    11521 tgcgatcatc atgtaccggc acaccccact cttcatcatg atattttatg taaagaggat
    11581 ttttcatatt tacccaatca cagcgattca tgatttacct cgtttgttaa aattattttt
    11641 aaattgaaaa actaaccagt agaagattta ttatttagtt acattctaat tatttgttta
    11701 taaagatttg ttttatttcc cctatcccaa tcttgatata tttctttaga cttatcagcg
    11761 cttagcggta gaattttagt ttttttgaat aatgcaaaga tttccatatt atatttttca
    11821 caaagccaac agtcccttcc atcattaaaa acgcttttca tggaattata tatttgaaca
    11881 aatggttgat cagcaatatt gccaaatgaa atatttaaga attcacatgg ttgtacatct
    11941 cctttcgcat tgatgtaaaa tctttcggtt cctcccgcgg tacaaccaaa catctctttt
    12001 gactcctcaa ttatttgtgc tgagattgac ggataatctt tatatcggct gccgttatta
    12061 tattcattga ctttgttttt cagtatttct aaatcttctt ttgaataatc cacgatgtcg
    12121 ctattgagcg ccgctcccgc ctctttcaat tttattacct gcaccatcgc tgcgccaagg
    12181 tcttttgcca tcttcattac tctatcaaat tctccattat gcaaatttgt tgccggtgga
    12241 caggtgttga tcgccacagg aactttattt ttatgacaca tttctatagc ttttatcatt
    12301 gtattaaatg ctcctttacg tttgaagaat ttatcatata tgtctttatc agtatacgtc
    12361 agcggaaaca ttattgcttt aacggggtat tcttttaaaa tatgcagccg ctcttctgtt
    12421 ataccgtctc ctgatgaatt tatccagata gaagagcgat catcaatttg ttcacatacc
    12481 actttaagcc gatcaaattt tataaatgga tctcctccct caatattaaa aaaagcaatt
    12541 cccatatcct gaagctgttt tacaacatta gtaagtttat caatgctgat atcctctcct
    12601 ttgtcaaatc gctggtaaca atggctgcac ctgtttttac atgcatttgt taccgaaatc
    12661 aatacggtgc tgcacggaag gcttttttca aactctttaa acttgttgca agaagtccag
    12721 aaagcctttg aaccaaagcc agggatatat agatcaatct ttgtttcacc attaattctt
    12781 acaaacttat tatgagatag ttttttgata agataaagaa gccgtcttaa aagacgcaca
    12841 aactttataa aggttatttt accctgaata agttctttag tgtagtgaaa tagaactttt
    12901 atttttattt gcagcaaaaa aatagttttg gaaatgcctg ttatattctt tttcataaaa
    12961 tccccatatt tttataccag tctgtagttc tttttatggc ttcttcaaca gaatattgag
    13021 gaatgaagcc cagttcttta tctatttttt ctgtactaaa ttcaagattg tcataaaatg
    13081 ttttcacacg tcctcgtgta agcagaggag gatttttaat ttttaataat gtataaataa
    13141 gttcaattat aaatgctact ggggaaatta tacaaagggg tattcgcacc ggacttttta
    13201 aaccgacata tttacaatac agttcaataa tatcctttat cttgattgaa ggtgaatatc
    13261 caacaaggta gttatttact ccagttattt ttttttcgta cacattatac attatacttg
    13321 caatatcttg tgcgtaggtg atgaacaggc tgttttttcc atacatggca acaggaaaag
    13381 tctcttttat aagtttaatt gtttcataaa atattgtgtt atactcacct tcaccgaata
    13441 tccagccggg atgtattgct gtaaggttta ttttgtactg gactgcgagt ttttgcgcct
    13501 caactaacgc aatttttttt gaatccctat agtagttcat cttacatgga aacagtgctt
    13561 ttagaaaata attatagtgc gaattaagcg gactgtgttc atctttaact gtataagaat
    13621 tttcttcacc atatactgaa atagaaccgg tcataataat atgatctata cggttttcag
    13681 cagctgcctc cagtacattg catgttcctg atatattaat atcttcaaag ctttgccagt
    13741 ctccccagtc actaacatgt gcagcgttat ggattacaca tttatgccct ttcatcaacg
    13801 ccgaaagcct gtctttgtcc cgaatatctc cataaactat agtaacaggt aaatcggcaa
    13861 ttgatgacgt atcactggtt tctcttacca tgcaagttac cggcacttta ttttttgtaa
    13921 aaaattcaac aatatgtgga ccaacaaaac cggttgctcc tgttataaac actttattca
    13981 taactgcagc tcttttgaaa ccatttgttc aacatctcaa tgtaatcttt catattttta
    14041 taatctcttg ctgaaaatga ataaccagta aaatcaattg taaaatgctc attagtaatt
    14101 ctttttgttt tttgttcaac aacagctcca acttcataaa attttacttt ttctgatctt
    14161 gcctcattaa taaactcatc atatctactt tttggcacag taaaacaaaa tgcatattca
    14221 ccagcctcgg caaatgcaag aagataacgt gaataccctg aaagtttttc aataatatta
    14281 cttccgctta caagtggaat gtctgttagt tcacatccat gatcacctgc atcgcaaagc
    14341 gattttatcg catgtaaaaa accgtcactt gtgtctgtac atgatgatgc aaacttttgc
    14401 attatcttta tctgtttata gtccagctca actctaaatg aagtcacttt atttagcaga
    14461 ggaagtttta aactttgcac tgctgcatta acattacaac ctcctatttt tctgcttata
    14521 aaaataatat cgccatgatt tgcgccagaa cgcattattg gttttacagt ttttccaata
    14581 acagaacttg taaaacgcca gtcacaatct atcgagaagt caccgcctat catgtaaaca
    14641 ttatgttctt tatacacacc actcattgcc ttattaagat ttactatata cttctcactc
    14701 catttactgc ttgcagtaag gctgtgcatt atccagagag gatcaccgcc tgacacatat
    14761 atatcggtta aagaagcgga ggcaaggttt ttacctaaaa gtgcgggatc actttcacca
    14821 aagtaatcat cttttgaaaa ctcatctatt gtacaaagga ggtaactgtc attgtctttt
    14881 attatttcag aatcagactc aaaacatctg ttaatctggt ttttatcata cggatacagt
    14941 tttctgattt tttcaattat gtttttttca atgttcattg aaccaccaga ggcaagaggt
    15001 ctgaacacat ttctatttta catggaagtt ctccaatact gtcacc
//
LOCUS       DAOTGZ010000044        14885 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_3064
            NODE_4827_length_14885_cov_4.757114, whole genome shotgun sequence.
ACCESSION   DAOTGZ010000044 DAOTGZ010000000
VERSION     DAOTGZ010000044.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868088
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 14885)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 14885)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:22:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,785
            CDSs (total)                      :: 2,751
            Genes (coding)                    :: 2,732
            CDSs (with protein)               :: 2,732
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 12 of 19
            Pseudo Genes (internal stop)      :: 5 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14885
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4827_length_14885_cov_4.757114"
                     /isolate="AMR_MDS_3064"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..401)
                     /locus_tag="P5123_04620"
     CDS             complement(<1..401)
                     /locus_tag="P5123_04620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017661092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarC family protein"
                     /protein_id="HRX15573.1"
                     /translation="MAEFIKSILLLLVLLNPFLVVIYLIDIIQKLDSKRFRNVLIRAG
                     FIAAAVFVCFSILGTAVFSSFFQVDFASFQIFGGIVFLLIGIQFVFKGPDAIEVLRGE
                     SEHIAGAVAMPVLIGPGTISASVVIGERHNLV"
     gene            complement(571..2850)
                     /locus_tag="P5123_04625"
     CDS             complement(571..2850)
                     /locus_tag="P5123_04625"
                     /inference="COORDINATES: protein motif:HMM:NF012742.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="substrate-binding domain-containing protein"
                     /protein_id="HRX15574.1"
                     /translation="MGNGKYKRFCLMLEMLDYGENYLEAIWKNTIKEARAAGVALIIF
                     VGQLIGTGKSDDEVQAALFKLARRPVFDGYILCGTVLNHIGKHNFSRIGKELANMPGV
                     VTSFAVPGLSTVSVDNESGLREVVRHLINDHGYRRIACIRGPKNNIEADLRFKVWKEE
                     IENAGLDESLDLLVQGHFNSRKSPDVYRDLKSLAGGMPQAVVACNDNMAIHIIEHLMK
                     DGYRVPEDIAVIGFDNIAHGLYLQCPLTTVHQPIGDTAVVALRKLIEETTNGCPSSET
                     VLPSTPVYRRSCGCANKHDNTSEPLPGLDSDSDKLETIFRSDAMNKTDMDYVRSAVAG
                     LHQKNWGILNLLELFNSFIHATALIDSVKELKSILHHWLPLFGIKTFSVGVCLTADGS
                     YESVKIKLMQEAIIAEPPEKFMLVACEPQIDGVIEGVTSYDSWELAPVEWLDNVGTTP
                     LVCLPLTVKDSWYGIALLEISGGDSMLYTSIQNHLATILDREYRLEKIVKMAIEEQTR
                     TYNEHEKLTSLSSLVRGMAHEINTPLGIGVTSSSYIAEKTGRRQKDFSENKITKAGLE
                     DYLKEVSDASEIVSKSLNRISNLVKTFKEIAVTESDHSNHKFNLCHILHQAIETNERI
                     LKAGNIVVKLELPDECTVFSSSDAFCKIVDQLICNVVEVSKEANREIILFVSLKAYSE
                     KDSLMILTFKDNGSGINESDLPHVFEPFFTTWRGSGHNGLGLHIVWNTVKYLLNGEIR
                     CESEKGKGTIVEIVFPKKV"
     gene            complement(2868..3971)
                     /locus_tag="P5123_04630"
     CDS             complement(2868..3971)
                     /locus_tag="P5123_04630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015360347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ldh family oxidoreductase"
                     /protein_id="HRX15575.1"
                     /translation="MSETIWWQFDEVERFMYEGFRAIGVPDHEAKVCANVLITADKRG
                     IDSHGVGRFKPIYLDRIWAGIQNPVTNFEIVRETDTTAVVDGHNGMGHYIAVKANEMA
                     VKKAKESGVGIVAVRNSTHYGAACYYPLMAIKEGCFGLTATNARPSIAPTWGVENMLG
                     TNPMTWGFPTDEDFPFVLDCATSVTQRGKIEYYGRLDKELPAGWVIGLDGKDRTDTKQ
                     VLEDLTKGKAALTPLGGLGEMTGGYKGYGYSIVVEILSAALSDADFLKALNGSDESGK
                     KIPILLGHFFMAVDISRFLPVERFKEITGTMMRQLRDSKKAPGAEKIYTPGEKEHEAW
                     LERKDKGVPFNEALIKSFREVKEKLNLKMDMPF"
     gene            complement(4167..5024)
                     /locus_tag="P5123_04635"
     CDS             complement(4167..5024)
                     /locus_tag="P5123_04635"
                     /inference="COORDINATES: protein motif:HMM:NF013862.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="patatin family protein"
                     /protein_id="HRX15576.1"
                     /translation="MKIYNIPLILEGGGLRCAFTAGILEFFLDNSIEFSHIYGVSAGA
                     SVAASYLAKQKGRNCRINVDMPSDPRFLGVCNFFRERSFFGMKYLYDEVPNHIDPFDF
                     KTFFETKTRFFTVVTSLQSGLPEYIENSRHDKQFSMKLLAASGSIPLLSPPVKLDGKF
                     YYDGGVSDSIPVKKALQQHDKAVVVLTRLRGYRKKSQKFKKYLRFRFRKYPEFVDVML
                     SRSDHYNSTLDYIDDMERKGKVLVIAPEDAAIMVERMERRRDKLKAFYDHGYDIINRR
                     INDLDKFSL"
     gene            complement(5094..6170)
                     /locus_tag="P5123_04640"
     CDS             complement(5094..6170)
                     /locus_tag="P5123_04640"
                     /inference="COORDINATES: protein motif:HMM:NF025025.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HRX15577.1"
                     /translation="MASVSIVVPAYKEAQNIPILVERLYTAFDSSDHSCSVIIVDDNS
                     MDGTVEAVSRLKKKYPVQLHVRTQERGLSSAVLAGFALSKGDIFICMDADLSHPPEKT
                     VEMCDLIEKEGADFVIGSRNVTGGSADSFNLYRKLNAFVSRMLARPLAKTSDPMAGFF
                     AFRSSLYTDQLVSMLNPVGWKICLEILVKMKPENVREIPIVFAERLYGESKLSLKEQI
                     NYLVHLKRLYYHKFKGLARFMVFSVVGASGIVVDLTIVFVVTDIAGAPFKYSRVAGIA
                     GAMTTNFILNRYFTFEERPARNIFASYVSFALVCSLGAALNWYVSMKMHSGFSMHYII
                     ASLTGIVAGTFVNYAGSTLLVFRK"
     gene            6485..7048
                     /locus_tag="P5123_04645"
     CDS             6485..7048
                     /locus_tag="P5123_04645"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor"
                     /protein_id="HRX15578.1"
                     /translation="MLSKEHYFIEAAKYKKALLKIAYLKIGNHFDAEDMVEEALLHAY
                     SKLDSLRDEEMLYPWLKRIVINHCNDLFRKSEKSDNSEKLLSLNKQENEDPEEKFTKN
                     SIREEILDIMLSIEPEEYAEVLILFFYRNFSYDEISDETSASVGTVKSRLSRAKEILL
                     KKFKEKGITSKDTELIGSISQWPTPST"
     gene            complement(7041..7466)
                     /locus_tag="P5123_04650"
     CDS             complement(7041..7466)
                     /locus_tag="P5123_04650"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRX15579.1"
                     /translation="MFIRKSKIDMIIQKEKRKEFLRSSAEFEKLHKKTVAELAQKQES
                     EIKDITKYYEKELKRKQQEILQLRQEISKNHDLYQKIRQRELELDNLSASFDTVLNEM
                     NIKVQESIQPFYRMRAKIETTKRISDKRDNKVQSIFQAM"
     gene            7662..8105
                     /locus_tag="P5123_04655"
     CDS             7662..8105
                     /locus_tag="P5123_04655"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRX15580.1"
                     /translation="MNINEFLKDKTVKIIFFRQIAYYREWVNALGVLNFNNSLVSYGD
                     YKKLTIRLIYKPASITEFLSSPFDSLKNGGKIVIELFGFKLSGDFLDKNKFSKKGNIT
                     SITVNTHRATVTIKGRPYSEGDEEFKTIEQIITDLSRFNVSQPDR"
     gene            complement(8080..11241)
                     /locus_tag="P5123_04660"
     CDS             complement(8080..11241)
                     /locus_tag="P5123_04660"
                     /inference="COORDINATES: protein motif:HMM:NF019924.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycogen/starch synthase"
                     /protein_id="HRX15581.1"
                     /translation="MVFCYGADGGLYPRSATDGRYGSWDDGKLLNYRRFEVWEWVHRS
                     IDTLVNKFGIDGIRFDSAHAVPIMMKRNNFPFVYDRSRTHEEMVEGRIIVNDRWDEHF
                     ITTGYYDCQCRDSIAVPFHQYLMYGIQRTLRKNKKDFFLYIAECFWGHERFLARSGIV
                     PYNASLFKVCENISHGKSDVREIYHIYDSYYPSVLPRGTELLGIFGNHDERRALNTFG
                     QHGLKAAISLTSFLSNIIMDYEGSAEGEGWKVFLDNIYVNWNQFEYASHRSVERFYRE
                     MYRFHSDNKGKGFLLWANNSMVAAALKPSDSGFWIGAFNFADSNQTASIQFDNPVLKI
                     DDNDFFRVVDPVYSGITGTYSYFTGKELKVSRVNTIISYVDRVKLLKLERQNSKTIDY
                     QLFFKDSFFRLCSISSGDKFLSNFAFCELSNAAVDSTSFEKFLMENILPVFWDESRYM
                     LFLGLKRVVYYLYKYDIVKGEQIFELIDHLVKSDDQRIVEIGEVLRKQNSRGEIVFMS
                     SEADPFSKSGGLANVVFELPREMAKMGEKVYVITGYYRNGDSKAVLKMTENAKKYGVT
                     YSGKNVRFKIMGSEYEVGVHIANVNGVTYYLLDHHELFDGLYWGVTSEEKLRRRIGFA
                     RACAEVICTFALRPYFTFTNDAFAALFNGVVRSDPNYSQHPSFSQNTYLHIIHNGGWQ
                     YFDAYERYENGFDLFSLFNLDGWKAGNFLDPVHPERINCMAAGIRFSDRVITVSPSYA
                     RQIEYQSDGLEHILSNVLGISNGVGADLYDRIKYRFDQSGFVDRNYKGLVKAVKADKH
                     LLEIVNSRFPEIIKGLKAVEDIKDDKRRYIVSRYMYKMMLQFERGFYIDPDKVLFCMI
                     HRITEQKGYQLLLDTSEGIFKTLQMQGVLGGSVSSGDRSGEEIGHGLWLLGQYYPKSA
                     NVGIGFQDVSVPLMASDIFCMPSLNEPGGISQLEGFLCGCLLVARATGGLRDTVFPIR
                     KNGDVIEGNGFLFSDYTGWAFYDAMERAVKFFRENSDDTIYEARMNARKSVFYWDIAA
                     SRYIENIYDMKEIVRINDRVVKH"
     gene            complement(11151..12965)
                     /locus_tag="P5123_04665"
     CDS             complement(11151..12965)
                     /locus_tag="P5123_04665"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-amylase family protein"
                     /protein_id="HRX15582.1"
                     /translation="MENESVKNEEICGLCRFALKDEHLVQAKYVKKEVAQSFEDGRAF
                     SFSVNKELIDDMRFERFVATYHERIIDIRNTIYDKAGEEDEYIKSRLVKDTRLAERLY
                     NAVFFLHKALADDDYNSLPERRFVILKERPGSPTIYHISNETTVISHVGQGPYWQEIP
                     TLYLGLKTFDILDTEYKRGETELFEAFKKLLKIEERAIETGFSHIEVYPADYSLALNN
                     LVNSLIEYTGQTELRAHEPAEKRDVKPFAQKIREKYLEMLDARYYHDPGHFDYAQNLK
                     AVEGIARLARRYRINGDRESLHECVRLLVSASGHDIHEIRNRANIVLERLFAPKQYDA
                     PLATVFYNLLAGDTFELTIKLPEAKNGYFVRIYNYSCDEDFFVDDDIDYKDYPMTKED
                     EVYAVSIPLNRYGHYDFLVFKKSSKKNEWVEQYGASGRINVMSDVRGEVILEIFTDIH
                     GHTKLYWRDENGHPGLVYNENGEVIRTGTFADITMHLEDMKRRYSISTIYILGCQKRG
                     THSEDWAQGATSPSPFSPESLTEIEESLGGADQFAELVRQAHLHEIKIIVDIIPHINR
                     HSSELSDEMWFSVMELMVVYTPDRLRTDVMEAGMMGNF"
     gene            complement(13193..13693)
                     /locus_tag="P5123_04670"
     CDS             complement(13193..13693)
                     /locus_tag="P5123_04670"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRX15583.1"
                     /translation="MKKCFKVVFLSFLSFGIFSCTTVDPLIKENNDFLSQSSGEAFRV
                     VMMSDSYLVQQKAYSSNMSRQEDTSGDSYFMERMKKLDVVNEKREGLIRVWVYPDSGR
                     LMKVRFLESTYIQEIDKLIIDDIQRWTFQYNRGISPLVFTVRYRVVLRKTMSDAEILD
                     EYEKHK"
     gene            13954..>14885
                     /locus_tag="P5123_04675"
     CDS             13954..>14885
                     /locus_tag="P5123_04675"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRX15584.1"
                     /translation="MKFKMLLFLAFFSLSTAIFASFPPEEDVTKARLTLSKYRWVQMH
                     YFLQTGYSMDFNESETYKSFYMGHNRLIFNGQTSEKMFFLFQTDDFVKKDSIDNTNGF
                     YTSDAFLHYRAHEAVQIYAGKMVAPVSRNALLSPATTVGISANHDALPSYTYTPEGRD
                     TGILIRGFLLNNLIEYRSGFFQGLGNKSFQYEDEGKEYTRNESSIPRITTRFQFNFID
                     PEEGYFYSENYLRKKNILALGLAFDYQPEVCDHNENGTFDDYFAVSSDISIQTDWRSA
                     NSFGFDLAFFYSKNNPNADIRYENLDYTDEIGFYV"
BASE COUNT         4780 a         3263 c         2400 g         4442 t
ORIGIN      
        1 acaagattat gtctttcacc aattacaaca ctcgcgctta ttgtaccagg gcctataagt
       61 acaggcatag caacagcacc agcaatatgt tcggactcac cacgaagcac ttcaatagca
      121 tctggacctt taaaaacaaa ttgtatacca ataagtaaaa aaacgattcc accgaaaatt
      181 tgaaatgatg cgaagtcgac ctggaaaaaa cttgaaaaca cagctgtacc caatattgaa
      241 aaacagacaa atacagcagc agctataaat ccggcccgta tcaaaacgtt gcgaaagcgt
      301 ttactgtcaa gtttctgaat aatatctatc aagtagataa caaccagaaa gggattcaaa
      361 agcaccagaa gaagtaaaat cgattttata aattcagcca tcatacatcc ttgtttagtt
      421 tttcactacg gttactaggc ctaatgaaca tgattatgtg ggcaagggtt ttttgtatat
      481 tagttcgaaa aaacgcttta ttcaaagttt tttaacctat ttcagctttt cttgacagtt
      541 tatctcttta ccactcgtat gattatcata ttatactttt tttggaaata caatttcaac
      601 tatagttccc ttaccttttt cggattcaca tctaatttct ccattcaaaa gatacttaac
      661 ggtattccag acaatatgta aacctaaccc gttatgaccg ctccctctcc acgttgtaaa
      721 aaaaggctca aacacatgtg gcaaatcaga ctcgttaata ccagatccgt tatctttaaa
      781 tgttaaaatc atcagtgaat ctttttcaga atatgctttt aacgaaacaa aaagaattat
      841 ttctctgtta gcctctttac ttacctctac aacattacat ataagctgat ccactatttt
      901 acaaaaagca tccgaactag aaaaaacagt gcactcatca ggtaactcaa gtttaacaac
      961 aatattgcca gctttcaaaa tccgctcatt tgtttcaatt gcctgatgca aaatatgaca
     1021 taaattaaac ttatgattag agtggtcaga ctcagtaacc gcaatttcct tgaatgtttt
     1081 tacaaggtta gaaattcgat tcagactttt ggatactatt tctgaagcat ccgaaacctc
     1141 ctttaaatag tcctcaagtc cagcttttgt aattttgttt tcactaaaat ctttttgtcg
     1201 cctacccgtt ttttctgcta tatagctact actggtaaca cctataccca aaggagtatt
     1261 aatctcatgc gccattcccc tgacaagcga agataaagaa gtcaatttct catgctcatt
     1321 ataagttcgt gtctgctctt ctattgccat tttaactatt ttctcaagac gatactcccg
     1381 gtcaagaatt gttgccaaat gattctgaat actggtgtaa agcatggaat ccccaccgga
     1441 tatttcaaga agcgcaatac cataccagct atccttaaca gtcaaaggca gacatacaag
     1501 aggagtagtt cccacattat caagccactc tactggagcc agttcccatg agtcataaga
     1561 cgttactccc tcaataacac cgtctatttg tggttcgcat gcaacaagca taaatttctc
     1621 tggtggctcg gcaatgattg cttcttgcat taattttatc tttactgatt cgtaacttcc
     1681 atctgcagtt aaacatacac cgacactaaa cgttttgata ccaaaaagag gtagccaatg
     1741 atgtaaaatt gattttagct ccttaacaga gtctattaat gccgtagcat gaataaacga
     1801 gttaaatagt tcaagaagat taagtatacc ccagtttttc tggtgtaatc cggctacggc
     1861 cgatctaaca taatccatat cagttttgtt catcgcatcg gatcggaaga ttgtctctag
     1921 cttatcagaa tcggaatcaa gaccgggtaa aggctcggaa gtgttatcat gtttgtttgc
     1981 acaaccacaa cttcgtcgat atacaggtgt agagggcaac accgtctcgg aagaggggca
     2041 tccatttgta gtctcttcaa taagtttgcg caaagccaca acagccgtat cacctattgg
     2101 ctggtgaaca gttgttagcg gacattgcaa atagagcccg tgagcaatat tatcgaaacc
     2161 tataacagca atatcttcgg gtactctgta accatctttc ataagatgtt cgataatatg
     2221 tattgccata ttatcgttac aagctacaac agcttgtggc attccacctg ctaatgattt
     2281 taagtctctg taaacatctg ggctttttct tgagttgaaa tgaccttgta ccagtagatc
     2341 cagactctcg tccaagcccg cattttctat ttcctctttc cataccttaa agcgcagatc
     2401 ggcttcaata ttgtttttcg ggccacggat acatgcaatt cgcctatatc cgtgatcatt
     2461 aattaaatgt cgtacaactt ctctaaggcc gctctcgtta tctactgaaa ctgtacttaa
     2521 ccctggtaca gcgaatgaag ttacgactcc aggcatatta gcaagttctt tgcctatacg
     2581 agaaaaattg tgctttccta tatgattcaa cacagtacca caaagaatat atccgtcaaa
     2641 aactgggcgc ctagctaatt taaagagtgc cgcttgcacc tcgtcatcac tcttcccagt
     2701 accgattaat tgacccacaa aaattattag agctactcca gctgctcggg cttctttgat
     2761 ggtatttttc catattgcct caagataatt ttcgccatag tcaagcatct caagcattag
     2821 acaaaatctt ttatatttcc cattacccat ctttactccc acagctatta aaaaggcata
     2881 tccatcttta gattaagttt ctctttaacc tcgcggaaag attttatcaa tgcttcatta
     2941 aaaggaaccc ctttgtcttt gcgttcaagc caggcctcgt gctctttttc acctggtgta
     3001 tatatttttt cagcaccagg cgctttttta gaatcacgta attgtcgcat cattgttcct
     3061 gtaatttctt taaatctttc taccggtaaa aaacgactaa tatcaacagc cataaaaaaa
     3121 tgtccaagta aaatcggaat ctttttgccg ctctcatctg atccattaag tgcctttaag
     3181 aaatcggcat cagacagtgc agcagatagt atctcgacaa ctatggaata accgtaaccc
     3241 ttatagccgc cggtcatttc ccccaaacct ccaagaggtg taagtgcggc ttttcccttg
     3301 gtaagatctt ccaacacctg ttttgtatca gtacggtctt taccgtcaag accaattacc
     3361 caccctgccg gaagttcttt atcaagacga ccgtaatact caattttgcc acgttgtgtg
     3421 accgaggtag cacaatccag aacaaaggga aaatcctcgt ccgtaggaaa cccccatgtc
     3481 atgggatttg tgccaagcat attctctacc ccccatgtag gagctattga tggacgtgcg
     3541 tttgtcgcag taagaccgaa acacccctct ttaattgcca ttaaaggata ataacatgct
     3601 gctccataat gggttgaatt tcttaccgca actattccaa caccagattc tttggctttt
     3661 ttaaccgcca tctcgtttgc cttaactgca atatagtgcc ccataccgtt gtggccatcc
     3721 acaaccgctg ttgtatccgt ttctcgtaca atttcgaaat tggttaccgg attttgtatg
     3781 ccagcccata tacgatccag atatatcggt ttaaaacgac ctactccgtg agaatctata
     3841 cccctcttgt cagcagttat caatacattg gcacaaactt tggcttcatg atctggaact
     3901 ccaatagctc taaagccttc atacataaaa cgttcaacct cgtcaaattg ccaccagata
     3961 gtttcactca ttttaatccc cctttttaga tatgttaaca gaaactttgt tttattaaat
     4021 aataaagaaa acctctatac ttcgtaataa aaaattaaat aacttttctt attaagatat
     4081 ttcggttaac acgtacaacc agacataaac agtatacaat acgttttgag ggaattcaaa
     4141 ctaaaaaacc agatcaacag gaggcattag agggaaaatt tatcaagatc attaatacgc
     4201 ctatttatta tatcgtatcc atggtcataa aatgccttaa gtttatcgcg tcgtcgttcc
     4261 atacgctcta ccatgatagc ggcatcttcc ggagcaatca cgaggacttt tccctttctc
     4321 tccatatcgt caatataatc aagcgttgaa ttgtagtgat cacttcttga caacattaca
     4381 tcaacaaatt cgggatattt tctgaacctg aatctaagat attttttaaa tttctgactt
     4441 tttttacgat agccgcgaag cctagttaga actacaacag ccttatcatg ttgttgcaaa
     4501 gcttttttta caggtataga atctgataca ccaccatcat aatagaattt tccgtcaagt
     4561 ttaacaggag gagacagcag tgggatgctg ccacttgcag caagaagttt cattgaaaat
     4621 tgcttatcgt gcctgctatt ttctatatat tcgggaagac cactctgcag agaagttaca
     4681 actgtaaaga aacgggtttt tgtttcaaag aatgttttaa aatcgaaagg atcaatatgg
     4741 ttgggaactt catcgtacaa atatttcata ccgaaaaagc tgcgctctcg aaaaaaatta
     4801 cacaccccca gaaaccttgg atcagaaggc atatcgacat ttatacgaca attacgccct
     4861 ttctgttttg caagataact tgcagcaaca gaagctcccg ccgaaactcc gtaaatatgt
     4921 gaaaactcta tactgttatc cagaaagaat tcaagaatac cagcagtaaa tgcacacctt
     4981 aaaccaccac cttccaatat aagtggaatg ttatatattt tcattatgag tcaccaaatc
     5041 aataagttta gaaacctcaa ttccgccaag atttaccggt taacaagaaa tatctacttc
     5101 cgaaaaacca gcaatgtact tccggcataa tttacaaaag ttccagctac aatgcccgta
     5161 agcgaggcaa ttatgtagtg catactaaaa cccgaatgca tcttcatgga tacataccaa
     5221 tttaaagcag cacccaatga acaaactagc gcaaaggaaa cataagacgc aaaaatatta
     5281 cgagccggcc tttcttcaaa agtaaagtaa cgatttaata taaaattggt tgtcatggca
     5341 cccgcaatac cggcaacacg tgaatacttg aaaggagcac ccgcaatatc tgttacaaca
     5401 aaaacaattg ttaaatccac gactattcca cttgcaccaa ctacagaaaa gaccatgaag
     5461 cgcgctaaac ccttgaattt atgatagtaa agtcttttaa gatgtacaag atagttaatc
     5521 tgttctttta atgatagctt gctttcaccg tataaacgct cagcgaaaac gatcggaatt
     5581 tcacgtacat tttcaggctt cattttgacc agtatttcaa ggcatatttt ccaacctacg
     5641 gggttaagca tcgaaaccag ttgatcagta tatagagatg atcggaaagc gaaaaatccc
     5701 gccatagggt cgctggtttt tgcaagaggt cgcgcaagca ttcgcgaaac aaacgcatta
     5761 agttttcggt aaagattaaa actatcagcc gatcccccgg ttacgttacg acttcctatt
     5821 acaaaatctg ctccttcctt ttcaatcagg tcacacattt ccacggtctt ttcaggtgga
     5881 tgcgaaagat cggcatccat acagataaat atatctcctt ttgatagagc aaatcctgcc
     5941 aaaaccgccg aagaaagccc tctctcctga gtacgtacgt gtaattgaac aggatatttt
     6001 tttttaagcc ttgatactgc ctcaacagtt ccgtccatag aattatcatc aacaataatc
     6061 accgagcaac tatgatcact actatcaaat gcggtataaa gcctttcgac aagaatcgga
     6121 atattttgag cctctttata ggcaggtaca actatggaaa ctgaagccat atcatatatc
     6181 ctgtaatatt ttttatactt ttaaagacat taaaatccat aattatgaag ataattccat
     6241 caagatacct attctacgat tttgctgaat attggcaaga aataattatc aacttcaaaa
     6301 atcctttgga aaaccaaaca ccgatgtaac gtataactct caattcgtag tgcctaaatt
     6361 atttcgattt aacacgcata gagattgaca gacctaaaaa tagtgttaca attgtttctg
     6421 atattcataa acggtattta aaacagaaac tattataact gaatcattac aatcaaagga
     6481 aaatatgcta tctaaagaac attattttat tgaagcagca aaatacaaga aggcactttt
     6541 aaaaattgca tatcttaaaa ttggcaatca ttttgatgcc gaagacatgg tcgaagaggc
     6601 cctattacat gcctatagta aactcgatag tcttagggat gaggagatgc tttatccatg
     6661 gcttaaaaga atagtaatca accactgtaa cgatctattc agaaaatccg aaaaatctga
     6721 taattcagaa aagttactat cgctcaacaa gcaggaaaac gaagaccctg aagagaaatt
     6781 cacaaaaaac agcatccgtg aagaaattct tgatataatg ctatcaatcg aaccagaaga
     6841 gtatgccgaa gtacttatac ttttctttta cagaaatttc agttatgatg aaatatctga
     6901 tgaaacatcg gcatcagtcg gtacggtaaa atctagacta tcccgtgcaa aagaaatttt
     6961 attaaaaaaa ttcaaagaaa aaggaataac cagcaaagac accgaactta tcggctcaat
     7021 atcacaatgg ccaacaccct ctacatagcc tggaatattg attgaacctt gttgtcacgc
     7081 ttatcagata ttctttttgt agtttcaatt tttgcgcgca tacggtaaaa tggctgaatt
     7141 gattcctgaa ccttgatatt catctcatta agtacagtat caaaacttgc gctcagatta
     7201 tccagctcaa gttctcgttg tcgaatcttc tggtaaaggt catgattctt agatatttct
     7261 tgtctaagct gaagtatctc ctgctgctta cgcttaagct ctttttcata atactttgtg
     7321 atatctttta tttcactctc ctgcttttgc gcaagctctg cgacagtttt tttatgcagc
     7381 ttttcaaatt cggcacttga gcgtaaaaac tccttacgtt tttccttctg tataatcata
     7441 tcaattttgg actttcttat aaacattgca ccctccggta attccataaa caggaaccat
     7501 tggtgccaat gaaaaatata accccatatt gtcaatgtgt tttttttaaa aacgttagtt
     7561 aagcaacatc cttgcaatag ccgcttgaca tggaagtggt ttacccccta actgaatcag
     7621 gttaataata tgaatctatt actcatccaa aacggaaatc aatgaacatt aatgaatttt
     7681 tgaaagataa aactgtaaaa ataatttttt ttagacaaat tgcgtactac agggagtggg
     7741 taaatgctct tggtgtactg aacttcaaca actctctcgt ttcttacggt gactacaaga
     7801 aactaactat acgacttatt tataaaccgg cctccattac cgaatttctt tcttcaccct
     7861 tcgattcatt gaaaaatgga ggaaaaattg tcatagaact ttttgggttc aagctatctg
     7921 gtgattttct tgataaaaat aaattttcaa aaaaaggcaa catcacaagt attacagtca
     7981 atacacacag agctaccgtg accataaaag gtcgtccata cagtgaaggt gatgaagagt
     8041 ttaaaacaat cgagcaaatc atcaccgatc tatcccgttt taatgtttca caacccgatc
     8101 gttgattctt acaatttctt tcatatcata aatgttttcg atgtaacggg aggcagcaat
     8161 atcccagtaa aacacacttt ttctggcatt catacgcgcc tcatatattg tatcatcact
     8221 gttttcacgg aaaaatttca cagcacgttc catagcatca tagaaagccc acccggtgta
     8281 atctgagaag agaaacccat ttccttcaat aacatcacca ttctttctta ttggaaatac
     8341 cgtatcacgc aatcccccag tagctcttgc tacaagaagg caaccgcaaa gaaaaccttc
     8401 aagttgagat attcctccag gctcatttag ggatggcata caaaaaatat ccgaagccat
     8461 caaaggaaca ctaacatcct gaaatcctat acccacattg gcactcttcg gataatactg
     8521 ccctaaaagc caaagtccat gccctatctc ttcaccagat ctgtctccag acgaaacgga
     8581 acctcccaag actccctgca tttgaagagt tttaaaaata ccttccgaag tatctaaaag
     8641 tagctgatac cccttttgtt ctgtaattcg gtgaatcatg caaaagagaa ctttgtcagg
     8701 atcgatgtaa aatccccttt caaattgaag catcatttta tacatatagc gactaacaat
     8761 ataacggcgc ttatcatcct tgatatcctc aacagctttt aatcctttta ttatttccgg
     8821 aaacctcgag tttactattt ctaacagatg tttatcagcc tttacagcct taaccaaccc
     8881 tttataattt ctatcaacaa aacccgactg atcaaaacga tattttatcc tgtcgtaaag
     8941 atcagcgcca actccgttac tgattcctaa aacatttgat aaaatatgtt caagaccatc
     9001 actctgatac tctatctgtc ttgcataaga cgggctaaca gtaataactc tatctgaaaa
     9061 ccttatacca gcagccatac agttaatacg ttcgggatga accggatcaa gaaaattacc
     9121 cgctttccat ccgtccagat taaaaagtga aaaaagatca aaaccatttt cataacgttc
     9181 gtatgcgtca aaatactgcc atcccccgtt atgaataata tgcaaatagg tgttttgaga
     9241 gaaagaggga tgctggctgt agttcggatc agaacgcacc accccattaa aaagagcagc
     9301 aaaagcatca tttgtaaatg tgaaatatgg acgcaaggca aaagtgcaga ttacctcagc
     9361 gcatgcgcgg gcaaacccta tccggcgtct tagcttctct tctgatgtta caccccaata
     9421 aagcccatca aaaagttcat gatggtccag aagataatat gtaacaccgt taacatttgc
     9481 gatatgtaca ccaacctcat attcagagcc cattatctta aaacgaacat ttttacctga
     9541 ataggtaaca ccgtatttct tggcattttc ggtcattttt agtactgctt tcgaatctcc
     9601 atttcgataa tagccggtta ttacgtaaac cttttcgccc attttagcca tttctcttgg
     9661 aagttcaaaa accacatttg caagaccacc acttttcgaa aacggatccg cttccgaaga
     9721 cataaacaca atttctccgc gagaattttg tttgcgaaga acttctccga tttccactat
     9781 tctctgatca tctgacttaa caagatggtc tatcaattca aaaatctgct caccttttac
     9841 tatatcatat ttatataaat aatatactac gcgttttaat cccagaaaaa gcatataacg
     9901 cgattcatcc caaaacaccg gcaatatatt ttccattaag aacttttcaa aactggtact
     9961 atctacagcc gcattcgaga gctcacaaaa agcaaaatta gaaagaaatt tatctccgga
    10021 tgaaatagag cagagcctga aaaaagaatc tttgaaaaaa agctgataat cgatggtttt
    10081 actgttctga cgttccagct taagcaactt aactctatca acatatgaaa tgattgtatt
    10141 tactcttgat accttaagct ctttaccggt aaaatagctg tatgtcccgg ttataccaga
    10201 atatacagga tcaacaacac ggaaaaaatc attgtcatct atttttaaaa ccggattgtc
    10261 aaattgaata gaagcagtct ggttgctatc cgcgaaattg aaggctccta tccagaaacc
    10321 tgaatcagaa ggttttaatg ccgcagcgac catggagtta ttcgcccaga ggagaaaccc
    10381 tttacccttg ttatcactat gaaacctgta catctctcga taaaaacgct cgacacttcg
    10441 atgtgaagca tattcgaatt gattccagtt tacgtaaata ttgtcaagaa agaccttcca
    10501 gccttctccc tctgcacttc cttcgtaatc cataattata ttgcttaaaa acgaagtcag
    10561 cgatattgcc gcttttaagc cgtgctggcc gaaagtatta agtgcccgac gctcatcatg
    10621 attgccaaaa atccccaaaa gttcagttcc ccttggcaaa acggaaggat aatacgaatc
    10681 ataaatatga taaatttctc taacatcgga ttttccatga gaaatgtttt cgcacacctt
    10741 gaacagcgaa gcattataag gcactatgcc tgaacgagct aaaaaacgct catgccccca
    10801 aaaacattcc gctatatata gaaaaaaatc ttttttattc tttcgtaatg tacgctgtat
    10861 tccgtacatt aaatattgat ggaaaggaac agcaatggaa tcacggcatt gacaatcgta
    10921 atacccggtt gttataaaat gctcatccca ccggtcattt acgatgatac gcccttcaac
    10981 catctcttcg tgggtccggc ttctgtcata aacaaaggga aaattattgc gtttcatcat
    11041 tattggaaca gcgtgagccg aatcaaagcg tataccgtca attccaaact tgttaacaag
    11101 cgtatcaatt gagcgatgaa cccactccca tacctcaaaa cggcgataat ttagaagttt
    11161 cccatcatcc cagcttccat aacgtccgtc cgtagccgat cgggggtata aaccaccatc
    11221 agctccataa cagaaaacca catttcatct gacaactcac tgctatgacg atttatatga
    11281 gggatgatat caactataat cttaatttca tgaagatgag cctgtcttac aagctcggca
    11341 aactgatcag cgccaccaag agattcttca atttcagtca aactttccgg agaaaatgga
    11401 gaaggtgatg tcgcgccctg agcccaatct tcggaatggg ttcccctttt ctggcagcca
    11461 aggatataaa ttgtcgaaat tgaatacctc cgcttcatat cttcaagatg catagtaatg
    11521 tcggcaaaag ttccggttcg tataacctca ccgttctcgt tatacaccaa tcccggatga
    11581 ccattttcat cacgccagta taactttgta tgaccatgta tatcggtaaa tatttcgagt
    11641 ataacttcac cgcgcacatc agacataaca tttatacgac cggacgcccc atattgttca
    11701 acccactcat tttttttgga acttttttta aatacaagaa aatcataatg gccgtaacga
    11761 ttaagaggaa tcgaaacagc atatacttca tcctctttgg tcatgggata atctttgtaa
    11821 tctatatcat catctacaaa gaaatcttca tcacatgaat agttatatat tcgcacaaaa
    11881 tatccatttt ttgcttcagg caattttatc gttagctcaa aggtatcacc agccaacaga
    11941 ttgtagaaca ctgtagcgag gggggcatcg tactgcttgg gggcaaaaag tctttcaagt
    12001 acgatatttg ctctattgcg aatctcgtga atatcatgac cggacgcaga cacaagaagc
    12061 ctgacacatt catgaagtga ttcacgatca ccgtttattc tataacggcg agccaaacga
    12121 gctatgcctt ctactgcctt aagattttga gcataatcga agtgacctgg atcgtgataa
    12181 taacgcgcgt caagcatttc aagatacttt tcacgaatct tttgtgcaaa aggcttaaca
    12241 tctcttttct cagcaggctc gtgagcccta agttctgtct gcccagtgta ttctataaga
    12301 gaattaacaa gattattcag tgccaacgag taatcagcgg gataaacctc tatgtgtgaa
    12361 aatcctgtct cgattgccct ctcttcaatt tttaacaatt ttttaaaggc ctcaaaaagc
    12421 tcagtttcgc ctcgtttata ttctgtatca agaatatcaa aggtttttaa tccaagatat
    12481 agtgtcggaa tttcctgcca gtatggcccc tgaccaacat gcgagataac agttgtttca
    12541 ttggatatat gataaattgt aggagaccca ggacgctcct ttagtataac aaaccttctc
    12601 tctggcaaag agttataatc atcatccgcc agtgctttat gtaaaaaaaa caccgcatta
    12661 tataggcgct cggcaagacg cgtgtccttt acaagacgtg actttatata ttcatcttct
    12721 tccccggcct tgtcataaat tgtgttacga atatctatga ttctctcatg atatgttgca
    12781 acaaagcgtt cgaaacgcat atcatcgatc agttctttat taaccgaaaa actgaaagcc
    12841 ctgccatcct caaaagactg ggcaacttct tttttaacat attttgcctg aaccagatgt
    12901 tcatctttga gggcaaaacg gcacaacccg caaatttcct cattttttac gctttcgttt
    12961 tccattacaa taacccggtt acaggctccg aactaatttt tttccggaac ccccatgaag
    13021 ataattttaa aaaaaaataa acggcaaata aaaattttta cagcaggtgt tttgttcaaa
    13081 cttaatccac aaaacagtac actccatgaa atttaccaag ttataaaaaa atgtccacaa
    13141 caattatgtc gtggacaatt tttttcgtta tatgagattt gaacaatatc gactatttat
    13201 gtttttcgta ctcatcaaga atttcagcat ctgacattgt ttttcttaat acaacccgat
    13261 atcttaccgt aaaaaccaga ggagaaattc cacgattata ttgaaatgtc cagcgctgaa
    13321 tatcatcaat aattagttta tcaatttcct gtatataggt cgactcaaga aaacggactt
    13381 tcattaaccg gccactgtct ggataaaccc aaacacgtat aagcccttcc cttttttcat
    13441 taacaacatc aagttttttc attcgttcca taaaataact gtcacccgat gtatcttcct
    13501 gacgggacat gtttgatgaa tatgccttct gttgcaccag atagctatct gacatcatca
    13561 caacgcgaaa tgcttcacca ctactttgag acagaaaatc attattttcc ttaattaacg
    13621 gatccactgt agtacaagaa aaaatcccga aagataaaaa cgacaaaaaa acaaccttaa
    13681 aacacttttt cataacattc tccacacatt tacaatcaca gatcagcttt tgctaccaaa
    13741 atcaagataa aggtaactac cattatttta atataatata gtttcaacaa ctattttttg
    13801 ccaaacagct acaatcaacc ataacaaacc ccaataacaa aaatttttct tgccaaatag
    13861 gcagtacccg attttacaat accatgtggt tttattacca tttggtgaac cgtttgtgct
    13921 gcaaggtgca aacacaattc tatggaggat ccaatgaaat tcaaaatgct tcttttcctg
    13981 gcgttttttt ccctttccac tgccatattt gcctctttcc cgccggagga agatgttacc
    14041 aaagcaagat tgaccctcag taaatatcgc tgggtacaaa tgcactattt tttacagact
    14101 ggttattcga tggattttaa tgaaagtgag acttacaaat ccttttacat gggacataac
    14161 cggcttatct ttaacggaca gacttcggaa aagatgttct ttcttttcca aacagatgat
    14221 tttgtaaaaa aagatagtat cgataacaca aacggatttt acacaagcga tgctttttta
    14281 cactataggg ctcacgaagc tgtgcaaata tatgcgggca aaatggttgc gcccgtatca
    14341 agaaatgcgc ttctttcacc agcaactact gttggaataa gcgcaaatca tgacgcactt
    14401 ccttcttata cttatactcc ggaaggacgt gatacgggta tattaatcag aggttttctt
    14461 ctgaataatc ttattgaata ccgttctgga ttttttcagg gattgggaaa taagagtttc
    14521 caatacgaag atgaaggcaa agagtatact cgaaacgaga gttcgattcc aagaataaca
    14581 actcgttttc agtttaattt tattgatccc gaagagggat atttttacag cgaaaactac
    14641 cttcgcaaaa aaaatattct cgcattaggc ttggcttttg attatcaacc agaagtttgc
    14701 gaccataacg aaaacggcac ctttgacgac tatttcgcag tttcatctga tatttcgatt
    14761 caaacagact ggcgctccgc aaattctttt gggtttgatt tggcattttt ttacagcaaa
    14821 aacaacccaa atgccgacat acgttatgaa aatcttgatt atacagatga aattgggttt
    14881 tatgt
//
LOCUS       DAOTGZ010000045        14757 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_3064
            NODE_4881_length_14757_cov_4.802612, whole genome shotgun sequence.
ACCESSION   DAOTGZ010000045 DAOTGZ010000000
VERSION     DAOTGZ010000045.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868088
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 14757)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 14757)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:22:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,785
            CDSs (total)                      :: 2,751
            Genes (coding)                    :: 2,732
            CDSs (with protein)               :: 2,732
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 12 of 19
            Pseudo Genes (internal stop)      :: 5 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14757
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4881_length_14757_cov_4.802612"
                     /isolate="AMR_MDS_3064"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..120)
                     /locus_tag="P5123_04680"
     CDS             complement(<1..120)
                     /locus_tag="P5123_04680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007204533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylyltransferase/cytidyltransferase family
                     protein"
                     /protein_id="HRX15585.1"
                     /translation="MHIGIYPGSFDPVTNGHLDIVRRASSLCDKLIIAVARNQD"
     gene            complement(385..1758)
                     /locus_tag="P5123_04685"
     CDS             complement(385..1758)
                     /locus_tag="P5123_04685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017306063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase"
                     /protein_id="HRX15586.1"
                     /translation="MKSIKSVMENLEKYYRSGATIPVDFRIKTLRDLYNNIKENQNAI
                     IRALSRDLHKPAFETYATEIGIVLDEIRKTCSHLKKWARPKRVSTPMHQFYASSYIIK
                     EPYGTVLIISPWNYPFQLAISPLIGAVAAGNTVMIKPSEISPNTSKIMASIITKSFDS
                     GHVSVVQGDVKTSTEILKQKFDYIFFTGSTVVGRVVMKAAAENLTPVTLELGGKSPAL
                     VYKDANLSLAAKRIVWGKFMNAGQTCVAPDYCFIHSEIKEQFTEELCRVIKEFYGDDP
                     SKSPFYARMINQSHYTRVKSYIKQGKVICGGSSNDKSRYIEPTIIEGASPKSAVMCDE
                     IFGPILPVMFYDDISTALEFINARPKPLALYIFSASRGFQKHVLNETRAGGVTINDTV
                     VHLVNPKLPFGGVGHSGMGSYHGKYSFDTFSADKPVMIRSTKIDIPFRYPPYSKLKAA
                     LIKMFLH"
     gene            complement(1858..3213)
                     /locus_tag="P5123_04690"
     CDS             complement(1858..3213)
                     /locus_tag="P5123_04690"
                     /inference="COORDINATES: protein motif:HMM:TIGR00797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0042910 - xenobiotic transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006855 - xenobiotic transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MATE family efflux transporter"
                     /protein_id="HRX15587.1"
                     /translation="MLYGLFSDRHFLKKLFITGLPVAIEIFLISLLGLVDNFMIGQMG
                     SVTIAAVGLAFQIMFLLIVFLFGLNSGAAIFHAQYWGKRNLANIHRLLGVSLITGFAV
                     CGIVSLAVMIFPDVIMRLFTADREVIVIGSSYFRIVGPTYVFMAVGFAFSFACRCTGH
                     PFLPTLTSLLGVVLNTGLNYLLIFGNYGFPQMGVRGAALATVISNATSTILFVILVYK
                     RDLHIAAPVKELFDFSRAYVARVYKVILYSIVTTMSWGFGMIMYNMIFARISTEAIAA
                     FTILSVVSGLAFSFIDGFSTGTSIVIGNALGENEFDRAYLYGKRSLVLVMLFSLFVAT
                     VIFFVAPYIVAVYNIPDATRLLALRYIQIFSIIYIVKSQISTMVNGILKSGGDTRFIL
                     IIDPGVMYVAGVLGGFLAAFVFKVNPLWVYVIMNIEEAIKILIAFPRFISKKWVNHLA
                     D"
     gene            3468..4208
                     /locus_tag="P5123_04695"
     CDS             3468..4208
                     /locus_tag="P5123_04695"
                     /EC_number="5.4.99.-"
                     /inference="COORDINATES: protein motif:HMM:NF013045.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HRX15588.1"
                     /translation="MRIEDHIIHEDNHLFVINKPEGMLAQGDSSERQSLNEIIKEYIK
                     ARDKKPGNVFLGIVQRLDRQVSGAIVYAKTSKAASRLSKQIRDHEICKYYICITSSKL
                     ATDTPGKWVEVEKNFIRRGNKTFKANLSDKEAKIGKLYYTTVAHNSRYSLHIVRLITG
                     RKHQIRATFNILDNPIADDALYGGSKEQNNSILLHSFYLKFRHPVNGTELTFVTDIPN
                     RFINYTDFNINRSDLCALIIKTIDNFKI"
     gene            4443..4781
                     /locus_tag="P5123_04700"
     CDS             4443..4781
                     /locus_tag="P5123_04700"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRX15589.1"
                     /translation="MKSFEEVKEKVFMVTRKFASTTGDYAHLTRMTMEIKGFETKIYT
                     LNSKLGKHIFESVEEERFEMDEQVREYCNKIREYKVEIQKRKDEIALIREKAKMSNDS
                     QSENNESETK"
     gene            4797..6998
                     /locus_tag="P5123_04705"
     CDS             4797..6998
                     /locus_tag="P5123_04705"
                     /inference="COORDINATES: protein motif:HMM:TIGR00358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016896 - exoribonuclease activity,
                     producing 5'-phosphomonoesters [Evidence IEA];
                     GO_process: GO:0006401 - RNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="VacB/RNase II family 3'-5' exoribonuclease"
                     /protein_id="HRX15590.1"
                     /translation="MPLSSKRIIEVLNSVSLPDFDQTTVRKQIIKESSKTKKGQIKNL
                     TKKEIAKINDIFFLLTSIGYLKTDGSKFTFAKDFDNNGQIRIGAGGKGYIDLGQFRIE
                     FRKENILNAHDRDTVAFEILEFRSNTFFGKVTAVKKRQKEIYYSKVTKKTAGMIYYHL
                     IDLPGQFFAVSPRHEKEPEIDTLAVVQLNGKLLAGHPECTLLRVDLGDNEELDFDRIV
                     NKFNLPPAYDKSDKREYANTFKNELSGRTDFTDLVTVTIDGEHAKDFDDAVSLEKTEN
                     GNRLYVHIADVSAYVTKGDSLDQSALERGNSYYLGNRVIPMLPETLSNNLCSLVAGQE
                     RLTVTAVIDYDHDLKILHTEFHRSYINVNKRLTYKDAEKLIDDDTDDISRLVTDLNSL
                     ASGLKKMRISNGRIDLDITDFELLYDEDNRFTSIGVSERLRSHLLIEECMLAANEAVA
                     KLLRKKDIPALYRVHEEISDESWFKLKNFLAVMGIKVKKTKKVSSVLQAVVDNYRGSD
                     YSYLVNLIILKSMMQAFYGVTPLGHFGLGFKDYTHFTSPIRRYSDLIIHRILKSHIQS
                     DEIVYTKDELLPIGEKCSEMERVAQKAERELYKIKSCRLMEHNIGDNFTAVISGISKF
                     GLYAVLKEKPVEGMIPFVLMNDDYYVVNEENYTVRGRSKGRIISLGDILTVTLVKADH
                     LSGQIDFIPADIRPQKKADNKETITQKDKKFYSEFEKKSRRKPKKSNRKKK"
     gene            7024..7491
                     /locus_tag="P5123_04710"
     CDS             7024..7491
                     /locus_tag="P5123_04710"
                     /inference="COORDINATES: protein motif:HMM:NF014912.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferritin family protein"
                     /protein_id="HRX15591.1"
                     /translation="MKYSLKEIIDMAVSIEDNGVYFYTKCADKFDDTSKKEFFLMLAD
                     EEKEHKKIFSSMLNTIPEAEGTFADEYYMYLNAITRQQVFKDIDSIDSYLEKVKSIGD
                     IFITAIHTEQDSIMWYRELLDMYKGDTKSIDVLNKLIAEEKKHIIRLYQVMNP"
     gene            complement(7596..8384)
                     /locus_tag="P5123_04715"
     CDS             complement(7596..8384)
                     /locus_tag="P5123_04715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015720688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="slipin family protein"
                     /protein_id="HRX15592.1"
                     /translation="MEQLFYENLTTIMFVMVLVVGFISSSVKIIKEYERAVVFRLGRV
                     LKQPKGPGLFLIIPFVDKLIRVSLRTVAMDVPSQDVISRDNVSIKVNAVVYFRVLDSI
                     KAVIEVEDYVYNTSQLAQTTLRSILGSVELDELLSDQEKINTDLQTVLDRQTDPWGIK
                     VSAVEIKHVDLPQEMQRAMAKQAEAERERRAKIIAAEGEQQAAEKMVQAAEQMEKHPI
                     SVQLRYLQTLQQIATENNSTTIFPIPMDMIMPFMEKVFSKNKKD"
     gene            complement(8386..9714)
                     /locus_tag="P5123_04720"
     CDS             complement(8386..9714)
                     /locus_tag="P5123_04720"
                     /inference="COORDINATES: protein motif:HMM:NF012783.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nodulation protein NfeD"
                     /protein_id="HRX15593.1"
                     /translation="MIRRSAIYSVFFCLFLNICSAAYAEETYARIDLKGSVNPISGAH
                     VVNSIKMAAETGDSFILFVIDTPGGLVNPMRDIIKAILASEIPVVVYTGPSGAQAASA
                     GAYIMLAGDVNAMASGSEIGAMHPYSPGISFSKEDSNDDQKDVMGMKVLNDMVAYGRS
                     LAQKRGRSIEWVEDAIINASSSSYKEAYEKGIIDIIADDERDLLQKLDGYTIDFANKS
                     FTFKTKGILESKYNMKSSQKFLNFFADPQLIMLLLIVGIGGIVFEIKQPGLIVPAVIG
                     VTSLILFFMAARILPVSIVGVILIAASVILLVMEVFITSYGLLTLGGVSAFVSGSLLL
                     FDNPLQGFSVSWMTIATTGLLILVLVFIVMRAVVAVHKTRVQVGPESLAGQIGTAATD
                     FVPASEGKVYVHGELWSARSDVSLSKGDNIEVTDIEGMILTVTKLIREEI"
     gene            10061..11311
                     /gene="hisS"
                     /locus_tag="P5123_04725"
     CDS             10061..11311
                     /gene="hisS"
                     /locus_tag="P5123_04725"
                     /EC_number="6.1.1.21"
                     /inference="COORDINATES: protein motif:HMM:TIGR00442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004821 - histidine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006427 - histidyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine--tRNA ligase"
                     /protein_id="HRX15594.1"
                     /translation="MNYTKAPGIEDIFPDRIELWNHIIDTARNVFKEYNIKEIILPIM
                     EFTEIFARGIGDETDIVSKEMFTFEDRGGRNLTLRPEGTASLVRAYCENGDYNRLASS
                     KFFYCGPMFRAEKPQKGRLRQFNQFGVEFFGEDSAYNDFEIIAVMDTITKRLGINDYT
                     LLINSIGNENERNIFVNELKKYYSDKIEKLCDNCKRRLDKNALRILDCKEPGCNVLKK
                     DAPLINNFLDDESLKHHNELKRLLDNAGIKYIEDPLLVRGLDYYCKTTFEFVTDSLGG
                     QNTFAAGGRYNKLVEMFSGKPKPAVGFAAGIERLFLMLESSFEPETSLDIYIIHTGDN
                     ARPKASLTAYELRNAGISVDFDPVGGSFKSQMKKLNRENCRFVVIISDEELEGNYATV
                     RDAQTRKEVKIGFDTLIANIKDML"
     gene            11364..13298
                     /locus_tag="P5123_04730"
     CDS             11364..13298
                     /locus_tag="P5123_04730"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRX15595.1"
                     /translation="MKTPLSKIMESYPFLDESVAREAFIFDVLNELITVQKSVEKGQS
                     RYFATKDHMYKSRAIKMPFTPSRDKNILFLLDLLTSRYPEYITTLIHFEISVDEKSKK
                     LNVKEIEEYCYSLASEEIERLQKLRDEQTRKSISDLISKFRVFNLTRLMSEVQNKKPF
                     NRVLQKMANKDIFIYKNVEIRNNKFSPTMEICFHSKNYAAVKSSLNLQSFKTIMANYQ
                     DMINPRLRNYGIMNADFSDYKENKIDYILHIMLEDLKSTLTPKDSVLVANFKSLRDCI
                     IRVENALDPVKVYNNEILTFIRRNRVSTASDIAASILSIDESVVRSWGTEDQLKKEKI
                     QTFKDASGAEYYVYCPVLMQEFKNYYNLIRYDQDKFEQLSPTLQKSTSVKLNGITEIV
                     SRVSAQSDAPSILKISKEDIESLLELIEEYQAWLKQKELRKELSSDELRGSGRSGRSV
                     FGALISAIKSLFGIFSRDDISDKISSNGDDYSPISTAPGSSKKPLTKEAKTIYAQTRS
                     RKAPILALSEFIVLSKENDKLVDQIINDMRQNNLKIIMPVYNARKVLYPKRSSKLLIP
                     DIEYLLIDPAVIRTSESITNYVDSLVGFKLKDDIISGTVLINIEKYLRNIQRQQKAKL
                     RIKRAQHQHLKNKHDSDKNI"
     gene            complement(13320..13580)
                     /locus_tag="P5123_04735"
     CDS             complement(13320..13580)
                     /locus_tag="P5123_04735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013968640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon domain-containing protein"
                     /protein_id="HRX15596.1"
                     /translation="MPTYDYKCQDCGHVFEAFQSMSADPLEKCEKCGGTVKRIIGAGA
                     GIIFKGSGFYVNDYKSGKSNEPSSKNSAPSCACSGGGDCPKS"
     gene            complement(13662..>14757)
                     /locus_tag="P5123_04740"
     CDS             complement(13662..>14757)
                     /locus_tag="P5123_04740"
                     /inference="COORDINATES: protein motif:HMM:NF014385.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="TolC family protein"
                     /protein_id="HRX15597.1"
                     /translation="GGTTHTPALFVSVEQQLLKNGFGINDRKQLAMMKNGADIQREML
                     KYQISAVSAGLIIDIWQLALARESFKNASTKLAETRKVRAIVNENVRLGLSESFEQNY
                     YNALVSGAQISLDNARYNLESSRRKIRTLLNLDDSVDIGETVELVTTLPEINESALIN
                     TALARRSDYKTAQLMLKNARLETGLYENESLPELSVSGTVRAYGVQEEFGEATSDALS
                     FEGNYYEVRAKLTHPLSNPNQKVSLRDAHFKLRQAELGIQKSEREIKDEIKSKVDNVN
                     TSYGIYQKSKAVRIQFGAYYRKMQANLRRGRFTAETVKNGLDAYVDSRQNELQALVGY
                     NIALVQLDIATNSIFEKYGIDINSYLMNSK"
BASE COUNT         5065 a         2912 c         2546 g         4234 t
ORIGIN      
        1 gtcctgattg cgggcaaccg ctatgattaa cttatcacaa agcgacgagg cccgccttac
       61 tatatccagg tgcccgtttg taacaggatc gaatgaacca ggatatatac caatatgcat
      121 aattcctcaa caatgtaaga caatagcttg ttatagctga taaacccgct aatgtcaaga
      181 aaatttcaca tggttattcg gaaaaaagat ggcattatac caaagtacaa tgtcacttta
      241 taaagaaaca aaggtattag ccattacccg aaaattacct tttaaccact cgctaataac
      301 agcaaccgcc ccaaataaat ttttttgaca aattatcaga ctgaaatttt catttatctc
      361 gttaatcaca gtttattaac ttctttagtg caaaaacatc tttatcagag cagctttcaa
      421 cttggaatat ggtggatatc taaaaggaat atcgattttg gtagagcgta tcatcacagg
      481 tttatctgcc gagaacgtgt cgaagctgta cttcccatga taagaaccca taccactgtg
      541 acccactcca ccaaaaggca acttaggatt taccagatga acaaccgtat cattgatggt
      601 tacaccacct gcccgagtct catttaacac atgtttttga aaacctctcg aagccgaaaa
      661 tatataaagc gccagaggtt ttggccttgc attaataaat tcaagagccg tagagatatc
      721 atcataaaac ataacgggta aaattggtcc gaaaatttca tcacacatca cagcactttt
      781 tggagaagct ccctctataa ttgtaggttc tatatatcta gacttatcat tactagatcc
      841 accacaaata accttaccct gtttaatata agacttaaca cgcgtataat gtgactgatt
      901 tatcattctc gcataaaagg gacttttgga aggatcatct ccgtaaaatt cttttattac
      961 ccggcaaagc tcttctgtaa attgctcttt tatttcagaa tgaatgaaac agtagtctgg
     1021 agcaacacag gtttgaccgg cattcataaa cttgccccaa acaatcctct ttgccgcaag
     1081 actcagatta gcatccttat ataccagtgc cgggctcttt cctccaagct caagagtaac
     1141 aggtgtgaga ttttcggctg ccgctttcat gacaacacgg ccaacaacag tacttcctgt
     1201 aaagaagatg taatcaaact tctgttttag tatttcggtc gaagtcttga catccccctg
     1261 cacaacagag acatgcccag aatcaaaact ttttgtaatt atcgaagcca ttattttgga
     1321 ggtatttggg cttatttcgg aaggttttat cattacagtg tttccggcag cgactgcacc
     1381 aattaatggt gaaatcgcaa gttgaaaggg ataattccaa ggtgatatga taagaactgt
     1441 tccatagggt tcttttataa tatatgaaga cgcataaaat tgatgcatag gtgtcgaaac
     1501 ccgtttcgga cgagcccact ttttaagatg agaacaggtt tttctaatct catcaagcac
     1561 tattccaatt tcggtcgcgt aagtttcgaa ggcaggttta tgcaaatctc gagaaagagc
     1621 ccgaattatt gcattctgat tttcctttat attattataa agatctcgta atgtttttat
     1681 tctaaaatca acaggaatag tcgcacctga tctatagtac ttctcaagat tttccattac
     1741 ggactttata cttttcatct cattctccta tttttattat actgttcacc taatataatc
     1801 aaatttagtt taaatacaaa gtaacttata aaaattatct attccggaag ctatcatcta
     1861 atcagctaaa tggttaaccc atttttttga gataaatctc ggaaaagcaa tcaatatttt
     1921 aatcgcctct tcaatattca ttatcacata aacccaaaga ggattaacct taaaaacgaa
     1981 agccgcaaga aatcctccca gtactccagc aacatacatg actcccggat caataattaa
     2041 tataaaacgg gtatcgcccc cactttttag aataccgttt accattgtac taatctgaga
     2101 cttcactata tatataattg aaaaaatctg gatataacgt aatgcaagca atcgagttgc
     2161 atcgggtata ttgtacactg ctactatata cggagcaaca aaaaatatca cagtagcaac
     2221 aaataatgaa aataacatta caagcacaag agaacgcttg ccatacagat atgccctgtc
     2281 aaactcgttt tctcccaaag cattacctat tacaatcgaa gtacctgttg aaaaaccatc
     2341 aatgaaagag aaggctaacc cagaaaccac tgataatatg gtaaatgctg caattgcttc
     2401 agtcgagata cgagcaaaaa tcatattgta catgatcatt ccgaatcccc aagacattgt
     2461 tgttactatt gaataaagaa taaccttgta aaccctcgcg acataagctc tgctgaaatc
     2521 gaataattct tttacaggtg ccgcgatatg taaatcacgc ttataaacaa gaataacaaa
     2581 aaggatcgta cttgttgcgt tagaaataac agtcgccaac gcagcccctc tgacgcccat
     2641 ctgagggaat ccgtaatttc caaaaattaa cagatagttt aaacctgtat tcaaaacaac
     2701 accaagaagg gatgtcagtg taggcagaaa tggatgccct gtacatcggc atgcaaaaga
     2761 gaaagcaaag cccacagcca taaacacata tgttggtccg acaattctaa aatagctcga
     2821 tccaataact attacttccc tatcagctgt aaataatctc attattacat cgggaaagat
     2881 cataacggcc aaagaaacaa tcccgcagac cgcaaaaccc gttattagcg aaacacccaa
     2941 aagtctgtgt atattggcaa gattacgttt tccccaatac tgtgcatgaa atatggccgc
     3001 accagaattt agcccaaaca agaaaacaat taataaaaac atgatctgga aagcaagacc
     3061 aactgcggca atggtcacag aacccatttg cccaatcatg aaattatcta ccaaccctaa
     3121 taaagaaatt aaaaatatct ctatcgcaac agggagacct gtgataaaaa gtttttttaa
     3181 gaaatggcgg tcagaaaata gtccgtataa catgtaatcc tctggcaaaa aaaaatcagc
     3241 atcgtttaaa accaaatatt atttatctgt ttttcgaagc tttttttcga ctaaaaataa
     3301 tttatcaatt caacaacatc tataaggacg ttttgtcaac tttaaagatg ttttttgaaa
     3361 ttatcattca atagaaaccg ctaatttgtt ataaaccgag caagatttca attagcgaat
     3421 cgtataatat tctttacttt tttcaacaac aggtagcgga tgtcattatg cgaattgaag
     3481 accatattat acatgaagac aatcatctgt ttgtaataaa caagccggaa gggatgctcg
     3541 cgcaaggcga cagttccgaa aggcagtccc tgaacgagat aataaaagaa tatataaaag
     3601 ctcgagacaa aaaacctgga aatgtttttt taggaatcgt tcaacggctt gataggcaag
     3661 tttcaggagc aatcgtatat gccaagacct ctaaagcagc ttcacgtctg agcaaacaga
     3721 tacgcgatca tgaaatatgt aaatattata tctgtataac ttcatcaaag ctggcaacag
     3781 acactcctgg caaatgggta gaagttgaga aaaattttat tcgcagaggt aacaaaacat
     3841 tcaaagccaa tttatctgat aaagaggcta aaataggtaa attatattat acaactgttg
     3901 cgcataacag cagatacagc ctacatatcg ttaggctcat cactggacgt aagcatcaga
     3961 taagagcaac gttcaatatt ctggataatc ctatcgcaga cgatgctctc tatggaggat
     4021 caaaagaaca aaataattca attcttttac actcttttta tcttaaattc agacatcctg
     4081 ttaacggaac cgagttaact tttgtaactg atattcctaa cagattcatc aattacacag
     4141 atttcaatat aaaccgatct gatttatgtg ctcttataat caaaaccata gataatttca
     4201 aaatatagag tattgagaat taccgaaaat cgtggtaact acatctatgt aacaaaacta
     4261 tatatttgat gccctgacat attaaacatc tggttgacaa ttatctttag attactatat
     4321 tctttgtata cacttaatga tttaatttta actcggtaat tttttttatt tattttattt
     4381 taaaatctgc ataagattag attcagactt ctgtgtcaaa aaaatctgta ctaggagact
     4441 atatgaaaag ctttgaagaa gttaaagaaa aggtttttat ggtaacaaga aaatttgcat
     4501 caactaccgg ggattacgct cacctaaccc gaatgacaat ggaaataaaa ggtttcgaga
     4561 caaaaatcta tacattaaat tccaaactag gaaaacatat ttttgaatca gtcgaagaag
     4621 agagattcga aatggacgag caggtacgag aatattgcaa taaaatacgg gaatataagg
     4681 tcgaaataca gaagcgcaag gacgaaatag cgcttatcag ggaaaaagcc aaaatgagta
     4741 atgattcgca atctgaaaat aatgaaagtg aaactaaata gttaacagga tataaaatgc
     4801 ccctctcctc caaacgaata attgaagtgc tcaactcggt ctctctacct gattttgatc
     4861 agacaactgt tagaaaacag ataatcaaag aatcgtccaa aacaaaaaaa ggacagatta
     4921 aaaatctgac aaaaaaagag atcgccaaaa taaacgacat cttttttctg ctaacatcaa
     4981 ttggttacct taaaactgac ggatcaaagt ttactttcgc aaaagatttt gataacaacg
     5041 gacagatcag gataggagct ggcggaaaag gatatattga cttaggccag ttcagaattg
     5101 aatttcgtaa agaaaacata ttaaacgccc atgatcgaga cactgtagct tttgagattt
     5161 tagaattcag aagcaatact tttttcggaa aagtaaccgc tgtaaaaaag cgtcaaaagg
     5221 agatctatta ctcaaaagtc acaaaaaaaa ctgcgggaat gatatactac caccttattg
     5281 atcttccagg acagtttttt gctgtatccc ctcgacacga gaaagaaccg gaaattgata
     5341 ctctagcggt tgtgcagtta aacggcaaac tgcttgctgg tcatcccgag tgcactcttt
     5401 taagggttga cttgggtgac aatgaggagc tcgattttga cagaattgtt aataaattca
     5461 acttgccacc ggcttacgat aaatcagata agagagagta cgcaaataca tttaaaaatg
     5521 agctttctgg ccgaacagac tttactgatc tggttactgt tacgattgac ggagaacacg
     5581 ccaaagattt tgacgatgcc gtctctttag aaaaaacaga aaacggaaac cgtctttatg
     5641 ttcatatcgc tgacgtatcg gcatacgtaa caaaaggaga cagccttgat caaagcgctc
     5701 ttgaaagagg taactcttat tatcttggta accgcgttat accaatgctt cctgaaacac
     5761 tgtcaaacaa tctatgctct ttagtcgctg gtcaggaaag acttaccgta accgcagtga
     5821 ttgattatga tcacgaccta aaaatacttc acaccgagtt tcaccgctcc tatattaatg
     5881 ttaacaaaag acttacctat aaggacgcag aaaaacttat tgatgatgat accgatgata
     5941 tttccagact agttacagat cttaacagtc ttgcttctgg tcttaaaaaa atgagaatca
     6001 gcaatggccg aattgatctt gacataacag attttgaact tctttatgat gaagacaatc
     6061 gttttacctc gataggcgtt tccgaaagat tacgttctca tctactaatc gaagagtgta
     6121 tgctcgccgc gaatgaagca gttgcaaaac tgttaaggaa aaaagatata cctgctctct
     6181 acagagttca cgaagagata tctgatgaaa gttggtttaa actcaaaaac tttctggcag
     6241 ttatgggtat caaggttaaa aaaactaaaa aagtgtcttc cgtacttcag gcggttgtgg
     6301 acaattatcg tggaagcgat tactcttacc ttgtaaacct tattattcta aaatcgatga
     6361 tgcaagcctt ttatggtgtt actccactag gtcattttgg gctgggcttt aaagattaca
     6421 cacatttcac atcaccaata cgccgctatt ctgatctaat aattcatcga atactcaaat
     6481 cacatataca atccgacgaa atagtataca ctaaagatga acttttacca ataggtgaaa
     6541 agtgttctga aatggagcgt gttgcccaaa aagctgaaag agagctttac aagataaaat
     6601 catgtagact tatggaacac aacattggtg acaattttac tgctgttatt tctggcatat
     6661 ccaagtttgg gctttatgct gttttaaaag aaaagcctgt cgaaggcatg ataccatttg
     6721 tacttatgaa tgacgattat tatgtagtta acgaagagaa ttatacagta cgagggcgga
     6781 gcaaaggaag aattatttct ttaggtgata ttttaactgt tactcttgtt aaagctgacc
     6841 atctatccgg acagattgat tttattcccg cagacattag acctcaaaaa aaagctgaca
     6901 ataaagagac aattacacaa aaggataaaa aattctattc tgaatttgaa aaaaagagca
     6961 gacgcaaacc aaaaaaaagt aatcgcaaaa aaaagtagcc aacaataagg aggaaccata
     7021 catatgaaat attcactaaa agaaataatt gatatggcag tatcaattga agataacggg
     7081 gtatattttt atacaaagtg cgcggataaa tttgatgata cttccaaaaa ggaattcttt
     7141 ttaatgctag ctgatgaaga aaaagagcat aaaaaaattt tttcatccat gcttaacact
     7201 attcctgaag ctgaagggac ttttgccgat gagtactata tgtacctaaa tgcgataact
     7261 agacaacagg tatttaaaga cattgactcc attgatagct accttgaaaa agtaaaaagc
     7321 ataggagata tatttatcac agcaatacat acagaacagg attctattat gtggtacaga
     7381 gaactattag acatgtacaa aggtgataca aagtcgattg acgtgttaaa taaattgatc
     7441 gctgaagaga aaaagcacat catccgcctt tatcaggtaa tgaaccctta aacaaaattc
     7501 atattatcca atcattacag aaaaacacaa accagataca ataatttgat tcgtggtata
     7561 ataaaagccg gcgttaccgc cggctttcgt atttattagt cttttttgtt ttttgaaaaa
     7621 actttttcca taaatggcat aatcatatcc atgggtatcg gaaaaattgt tgttgaattg
     7681 ttttctgtcg caatttgctg aagagtctga agatatctta gttgtacaga aattggatgt
     7741 ttctccatct gttcagcagc ttgtaccatc ttttctgctg cctgttgctc accttcagca
     7801 gcaattattt ttgcacgacg ctcacgctcg gcttctgcct gctttgccat ggctcgctgc
     7861 atctcctgtg gcaaatcaac atgctttatc tctactgcag aaaccttgat cccccaggga
     7921 tcagtctgcc tgtcaagaac agtctgtaaa tccgtgttta ttttttcctg atccgaaagt
     7981 agttcatcta attcaacact tccgagtatt gaccgtaatg tagtttgcgc taactgtgat
     8041 gtattgtaca cataatcttc aacttcgata acagctttga tagaatcaag aactctaaaa
     8101 tagacgacag cgttaacctt gattgaaacg ttatcacgac ttattacgtc ctgtgaggga
     8161 acatccattg ctactgtacg cagcgaaaca cgaattaatt tatccacaaa aggaataata
     8221 agaaaaagcc ccggtccttt tggctgttta agaacacgac caagacgaaa tacaactgct
     8281 cgttcatact ctttgattat cttcacggaa gaacttataa aaccaaccac taaaaccata
     8341 acaaacataa ttgttgtcag attttcataa aacaactgtt ccatattaaa tctcctctct
     8401 tattaatttg gtaactgtta atatcattcc ttcaatatcg gttacctcta tattatcacc
     8461 ctttgataac gagacatcag atcgggcact ccacagctct ccatgtacat aaaccttgcc
     8521 ctcggaagca ggaacaaaat ctgttgccgc ggttccaatt tgaccagcaa ggctttcagg
     8581 ccctacctga actcttgtct tgtgaactgc aacaacagca cgcataacaa taaaaacaag
     8641 caccagtatt aataatccgg tagtagcaat agtcatccat gagacagaaa agccctgtaa
     8701 cggattgtcg aaaagaagca atgatccgct aacaaaagca ctaacaccac ctagggtaag
     8761 aagtccgtag cttgtaataa atacctccat tactagcagt attacagaag ccgcaatcaa
     8821 aattacgccc actatagata caggtaatat tcgcgcagcc ataaaaaaaa gtattaatga
     8881 tgtaacacca ataacagcag gaacaatcaa gcccggttgt ttaatctcga atactatacc
     8941 gccaataccg actattaaaa gcagcatgat caattgagga tcggcgaaaa aattcagaaa
     9001 cttctgagag ctcttcatat tatatttact ctccaaaata cctttagttt taaaagtaaa
     9061 cgatttattc gcgaaatcaa ttgtataccc atcaagcttt tgcaaaagat cacgctcgtc
     9121 atctgcaatg atgtctatta tgcctttttc gtatgcctcc ttatagctac tcgaagaggc
     9181 gtttataatg gcatcttcaa cccactctat gcttcgtccc ctcttttgtg caagacttct
     9241 tccataggca accatatcgt tgagcacctt catgcccatt acatccttct gatcatcatt
     9301 cgaatcttct ttagaaaaag atatccccgg ggaataggga tgcatcgctc cgatttcgct
     9361 tcctgacgcc atagcgttca catcacccgc aagcattata tatgctccag ccgatgccgc
     9421 ttgcgcgccg cttggaccag tatatacaac aacaggaatt tcgcttgcta aaattgcttt
     9481 tatgatatcc ctcatcgggt taacaagccc cccaggtgta tctattacaa aaagaataaa
     9541 actgtcaccc gtctcggctg ccattttaat cgaatttacc acatgagcac cacttattgg
     9601 attaaccgaa ccttttaagt ctatgcgggc ataggtttct tcggcataag cagcagaaca
     9661 gatattcaga aataaacaga aaaaaacaga atatattgct gatcttctaa tcaaaatcta
     9721 atctccagtt actttttgtt aataaatcca gtttaagaaa aaattgtcat aaaacaagtt
     9781 atcattaatt atatgcttag gatcatcaaa gtcaacctat atttttaatt cgcattctgc
     9841 gatatagagt tagaacaatt taaacttcaa gccgaatata gcaatactga ctcactatta
     9901 atctatatat gatgatataa aagatttatt ttgacaaaaa tgcacactca tttattgttg
     9961 tgttctaaac tattagtatt gctgttcaag tcaaaatagc ctttattgtt aaagttatag
    10021 ctacgattca atgtggcttt ctagcttcaa ggatatctat atgaattata caaaagcacc
    10081 cggaatagaa gacatcttcc ctgacagaat tgaactctgg aaccatatca ttgacacagc
    10141 tcgcaacgta tttaaagaat acaatatcaa ggaaattatt cttcctatca tggagtttac
    10201 cgaaatattc gctcgtggaa tcggtgatga aacggatatt gtttccaaag agatgtttac
    10261 gtttgaagac cgaggcggcc gaaaccttac actgcgccca gaaggaacgg catcacttgt
    10321 acgtgcctat tgcgaaaacg gagactataa ccgtcttgcc agtagcaaat ttttttactg
    10381 cggtcccatg tttagagccg agaaaccaca aaaaggaagg cttcggcagt ttaaccagtt
    10441 cggtgttgaa ttttttggtg aagattccgc atataatgat ttcgagataa ttgcagtaat
    10501 ggacaccatc acaaaaagac taggaattaa cgattatacg cttctgataa actctattgg
    10561 taacgaaaac gaaagaaata tttttgtaaa tgaactaaaa aaatattata gtgataaaat
    10621 tgaaaaactt tgtgataatt gcaaacgacg tcttgataaa aacgctcttc gaatacttga
    10681 ttgcaaagaa ccgggctgta atgtactaaa aaaagatgct ccattaatca ataactttct
    10741 tgatgatgaa agccttaaac atcacaatga gcttaaaaga ttgctcgata acgccggtat
    10801 aaaatacatt gaagaccctc ttttggttag aggtctggat tactattgca aaacaacctt
    10861 tgaatttgtt acagacagcc ttggtggtca aaataccttt gcagctggcg gacgctacaa
    10921 caaacttgtc gagatgttca gtggaaaacc aaaaccggcg gtaggttttg cggccggcat
    10981 agaacgcctt ttccttatgc ttgaatcaag ttttgagccc gaaacatctc ttgatattta
    11041 catcatacat actggcgata atgcgagacc aaaagccagc ctaacggcgt atgaacttag
    11101 aaatgccggg atcagtgtag attttgaccc ggttggtgga agctttaaat cacaaatgaa
    11161 gaagcttaac cgtgaaaatt gccgttttgt cgtaatcata tctgatgagg aactggaagg
    11221 caattacgct acagtcagag acgcacaaac acgaaaagaa gttaaaattg ggtttgacac
    11281 tttgattgcc aatataaagg atatgttgta gaatatagct atcaatcagt tttttaacgt
    11341 taaatgacac cataaggatc tttatgaaaa caccgctttc aaaaataatg gagtcgtacc
    11401 cttttcttga tgaatctgtt gccagggaag catttatttt tgatgtgcta aacgaattaa
    11461 ttacggttca aaaaagtgtc gaaaaaggtc aatcacgtta ttttgcaaca aaagaccata
    11521 tgtacaaatc acgtgccata aagatgccgt ttacaccctc tagagacaag aatatacttt
    11581 ttttactgga tctattaaca tctagatatc cagaatatat aacaacatta atacacttcg
    11641 aaatatctgt agatgaaaaa agtaaaaaat taaacgtcaa agaaatcgag gaatactgct
    11701 actcccttgc atcggaagag attgaacggc ttcaaaagct tcgagacgaa cagactcgca
    11761 aatctatttc agatcttata tcaaaattca gggtatttaa ccttacccgt ttaatgagcg
    11821 aggtgcaaaa caaaaagcct tttaaccgtg tactgcaaaa gatggctaac aaagatattt
    11881 ttatctataa aaatgttgaa atcagaaaca acaagttctc tccaacaatg gaaatatgct
    11941 ttcattcaaa gaactatgct gctgtaaaaa gctccctcaa tctgcagtcc tttaagacca
    12001 taatggccaa ctatcaggat atgattaacc ctcgtcttcg aaactatgga attatgaacg
    12061 cagatttttc tgactataaa gagaacaaga tagactatat actgcacata atgcttgagg
    12121 atctgaaatc gacacttacg cctaaagatt cagttcttgt tgccaatttt aaatcgctca
    12181 gagactgcat tatacgtgta gaaaacgcac ttgatcctgt taaggtttac aataacgaga
    12241 tattgacctt tattagacgc aatagagttt caaccgcatc tgatatcgca gcttctattt
    12301 tatcgataga tgaatcagtt gtaagaagtt ggggcaccga agatcagctt aaaaaagaga
    12361 agattcaaac gtttaaagat gcttctggag ctgaatatta cgtatattgc cctgttttga
    12421 tgcaagaatt taaaaattac tacaatctga taagatatga tcaggataaa tttgaacagc
    12481 tttctccaac cctgcagaaa agtacatccg ttaaactaaa cggaattacc gaaatagtat
    12541 cacgagtatc ggcccaaagt gacgccccct caatacttaa aatttccaaa gaagatattg
    12601 aatctttatt agagctaatc gaggaatatc aggcttggct aaaacagaaa gagttgcgca
    12661 aagaactatc tagtgacgaa cttagaggtt caggacgcag cgggcgttct gtttttggtg
    12721 ctcttataag cgctataaag tctctattcg gtattttttc aagagacgac atatcggaca
    12781 aaatttcgag taacggcgac gattattcac ctatatcgac tgccccagga tcgtcaaaaa
    12841 aacctctaac aaaagaggct aaaactatct atgctcaaac aagatcacga aaagccccaa
    12901 ttcttgcatt atcagaattt attgttttaa gtaaagagaa cgacaaactg gtagaccaga
    12961 taatcaatga tatgcgtcaa aacaacctga agattataat gcctgtctac aatgcacgaa
    13021 aagttcttta ccctaaaaga agttccaaac tgctgattcc tgatattgag tatcttctta
    13081 ttgatccagc agtcattcga acatcagagt ctatcaccaa ttatgtagat tctcttgtag
    13141 gctttaaatt aaaagatgat attatttcag gaacagtact gataaatatc gaaaaatatt
    13201 tgagaaatat ccaaaggcaa caaaaagcaa agcttcgcat aaaaagagca cagcatcaac
    13261 atttaaagaa taaacacgat tcagataaaa atatttagag aaaaaattag agtatttgat
    13321 caagatttgg ggcaatcgcc tccacccgaa catgcgcatg atggtgcact gttttttgaa
    13381 gacggttcat tactctttcc cgacttataa tcattaacat aaaatccaga gcccttgaat
    13441 attattccgg cacctgctcc tattattcgc ttaaccgtgc ccccgcactt ttcacatttt
    13501 tcaaggggat ctgcactcat cgactgaaaa gcttcaaata catgtccaca atcctggcat
    13561 ttgtaatcat aagtaggcat aagtacctct caaaaaaagt attaatttgg atgattttca
    13621 gacataaaaa ttcaagtcaa tatttgtcaa gcacgaatag actatttcga attcattaaa
    13681 tacgaattga tatcaattcc atatttttca aaaatggaat ttgtcgcgat atcaagctga
    13741 acaagagcaa tattgtaacc taccaacgct tgaagctcat tttgacggct atctacatat
    13801 gcatcaaggc cattttttac cgtttccgct gtaaatcgtc cacgtctcag gttagcctgc
    13861 atctttctat aataagcacc aaactgtata cgaacagcct tgctcttctg gtaaataccg
    13921 tagcttgtat ttacattatc tactttactt ttaatctcgt cttttatctc gcgttcactt
    13981 ttttgtattc ccaattcagc ctgacgaagt ttaaaatgcg cgtctctcaa actcaccttt
    14041 tgattaggat ttgaaagtgg atgagtaagc tttgcgcgaa cttcatagta attaccctca
    14101 aaagaaagag catcacttgt ggcttcgcca aactcttctt gcacgccata cgcacggaca
    14161 gtgccggaaa cagagagctc gggcaatgat tcattctcat ataagccggt ctctaatctt
    14221 gcatttttta acattagttg agcagttttg taatcagatc gacgggctaa agcagtgttt
    14281 attaatgcag attcgttaat ctctggcaag gtagtgacaa gttcaactgt ctcaccgatg
    14341 tccacactat catcaaggtt aagaagagtt cttatctttc tacggctaga ctcaaggtta
    14401 taacgggcat tatcgagact tatttgcgct ccggaaacaa gagcattgta atagttttgt
    14461 tcaaaacttt cggataaacc aagtctaaca ttttcgttta caatggcgcg aacctttcga
    14521 gtttcggcaa gtttggtgct ggcgttttta aaagactcac gcgcaagagc aagctgccaa
    14581 atatcaataa taagaccggc cgaaacggct gatatttggt acttgagcat ttcccgctga
    14641 atatcggctc cgtttttcat cattgcaagc tgcttacggt catttatacc aaaaccattt
    14701 ttcaacagct gctgttccac cgacacaaaa agagcaggtg tatgtgttgt cccaccg
//
LOCUS       DAOTGZ010000046        14623 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_3064
            NODE_4941_length_14623_cov_4.276153, whole genome shotgun sequence.
ACCESSION   DAOTGZ010000046 DAOTGZ010000000
VERSION     DAOTGZ010000046.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868088
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 14623)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 14623)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:22:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,785
            CDSs (total)                      :: 2,751
            Genes (coding)                    :: 2,732
            CDSs (with protein)               :: 2,732
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 12 of 19
            Pseudo Genes (internal stop)      :: 5 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14623
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4941_length_14623_cov_4.276153"
                     /isolate="AMR_MDS_3064"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(57..608)
                     /locus_tag="P5123_04745"
     CDS             complement(57..608)
                     /locus_tag="P5123_04745"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRX15598.1"
                     /translation="MKQISNKKTNFSEKQILNSKTPDEYIHRSLYSDVPRGRKTILCR
                     QWKEETGFTSEDIQYARNRHPYWKDKKMSGWQERNQKRWSEHDYRKVKKNRIEAFESE
                     VIEKFCSLNKKDKKGNYLMKDWEIAKELKLSIPSVQHWRRKYLLVLRLLEVLQKKPSK
                     KIILKYMGRHENGLRREYNELVS"
     gene            complement(1114..1920)
                     /gene="panB"
                     /locus_tag="P5123_04750"
     CDS             complement(1114..1920)
                     /gene="panB"
                     /locus_tag="P5123_04750"
                     /EC_number="2.1.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008908310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003864 - 3-methyl-2-oxobutanoate
                     hydroxymethyltransferase activity [Evidence IEA];
                     GO_process: GO:0015940 - pantothenate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-methyl-2-oxobutanoate
                     hydroxymethyltransferase"
                     /protein_id="HRX15599.1"
                     /translation="MKRKNITVNTMLDMKKQGEKISMITCYDYTMAKLVSRSGIDVIL
                     VGDSGGMVQAGYDNTLPVTVDEMVYHTAAVRRGAPNMFIVADLPFLSYHVSKEKTIEN
                     AGRLIQEGKANSVKLEGGSYFTELVSHLTACSIPVCGHLGMTPQSIHNFGGFRVQGRE
                     EDKARRMVDDAKRIEDAGAFALVLELVPRSLAKRICEALRIPVIGIGAGPDCDGQVLV
                     INDVLGLDDTFRPKMVKDYAGLAGKVITALDNYSQEVKQGDFPLDENSFE"
     gene            complement(1895..2362)
                     /gene="folK"
                     /locus_tag="P5123_04755"
     CDS             complement(1895..2362)
                     /gene="folK"
                     /locus_tag="P5123_04755"
                     /EC_number="2.7.6.3"
                     /inference="COORDINATES: protein motif:HMM:TIGR01498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003848 -
                     2-amino-4-hydroxy-6-hydroxymethyldihydropteridine
                     diphosphokinase activity [Evidence IEA];
                     GO_process: GO:0009396 - folic acid-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-amino-4-hydroxy-6-
                     hydroxymethyldihydropteridine diphosphokinase"
                     /protein_id="HRX15600.1"
                     /translation="MNKAYIGIGTNLGNRELNIIRACEEMNRCGITVLRQSSIENTKP
                     VLYENQPDFLNTVVYVSAECSANELLVKLKGIEVKLGRVPSVEKGPRLIDLDILLYNN
                     TVVECPDLVLPHPGIRERIFVLKHLLELNPDLSDPRDGVAYIEVYNEKKKHYC"
     gene            complement(2362..3177)
                     /locus_tag="P5123_04760"
     CDS             complement(2362..3177)
                     /locus_tag="P5123_04760"
                     /inference="COORDINATES: protein motif:HMM:NF013828.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HRX15601.1"
                     /translation="MGQDPVEKTKVVQFCQFCEGTGIIKNPDAYITGENPITPCPKCV
                     LPHCRCGGESPYLYAENGKISECPCNPVRRRINRINALFSKSEIDKKYRWLFLSDFNV
                     KTQADNKVKSAAYKIIHSFPDVSKGLFLWGNPGTGKTMISAIILTELISRYAVSGKYL
                     KISRNFFGRLRSTFVEGSQMYGQSSQIERDLAEIDVLVVDDFGVQRDSPWEQETLYNL
                     VDSRYEAEKFTIFTSNNNPAESMKELGGGRVLSRIKEMCYIMELSGSDHREEK"
     gene            3392..4237
                     /locus_tag="P5123_04765"
     CDS             3392..4237
                     /locus_tag="P5123_04765"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRX15602.1"
                     /translation="MKRKLCMTICALSVLTVLIFASSIYAQDTAAPAVDGDKVPSEMG
                     GQWRVEDFHPYVKALDDLRKLSKEYAEKIMIKSIDEYSIGMDILRDMDANLSNLQKQY
                     DRQNNLNERWYWQEIDRENQLKRRKGMLKYSAKLRAITYFTRSINSLDQIQSEEVRKD
                     PKFVEFQIRLFKAYVSAQYDIHNLKPCIPILERYIAINDNTKQDVQAYKYLASCYGYM
                     EKSLAKYNTTISEERVIKYKQKKNQALLMATEIEYGIESIEYRKLKDIVEIDEIQSER
                     INNFR"
     gene            complement(4358..5464)
                     /locus_tag="P5123_04770"
     CDS             complement(4358..5464)
                     /locus_tag="P5123_04770"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRX15603.1"
                     /translation="MIYYLNTDFDIANVRVPEGFLAQVYLLNLLPLALAEDDDVIVSH
                     VRPEENYYKYLAQISGDCGSLVIREECKQYLSSELYLWGINSDSLSLVCDPESYPGVI
                     NVAKANCRSFSLEVSKKMGSLIRREIVSSIKDVCVFAKNYDYPLVVKPLCGSSAYGFN
                     IVQTERCLEKIVIDNRAVCEQWVKRVSDFSYGFYISKEGEMSFLTVRSLDNSPRGIFS
                     SVFYYCNSCDSPLYGLIEKSAFGLSKVLYESGYFGPVNCDGFLYKSNNSIYKEICCEI
                     NARRSIADLAILCKKRFRVDCGGIVSIQQKVYSPELCQKLAEMFDNRNVRLLTPPRFY
                     YKNMLYRSRRLLFFVGGKDQNDCMEKTRRLKSLL"
     gene            complement(5591..7708)
                     /locus_tag="P5123_04775"
     CDS             complement(5591..7708)
                     /locus_tag="P5123_04775"
                     /EC_number="3.1.3.16"
                     /inference="COORDINATES: protein motif:HMM:NF018884.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004722 - protein serine/threonine
                     phosphatase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PP2C family protein-serine/threonine
                     phosphatase"
                     /protein_id="HRX15604.1"
                     /translation="MLYRAVLKALVPLCLVLLSVPMQGQNLPIDLTNRDFYVRQGFYP
                     EQISTFPPSVDWKKIVTAGYDRALTYPELFKDEAEIDDRQFTLLTSFHIDRHTSIRRS
                     LGISIPLIGSGFELYLNGKSLYNHFDIDTTLIFRNKVIEIPFYLLNDGFNVLCFRVKG
                     GNEDPVSGIITEGFPFSIDFFAKLQQYEYDFHLKIAILAAVIVFATGLLLLFLLISPS
                     DRRCIALSIGIMMAYLALLLADSHYLYLIFNISRFDLPVLYGALSFISLCSILLISFR
                     GGNRYSLWIACYLLLGFIAVVILLYFFGFNRLIFSVSLLLIALVSLLFNYNVIKNFFA
                     SPLAETFFERIFPFCIFFNYQMQTLLLVLLLFVGLIWFVASEHLLRPVVFIGLGSVII
                     TIASSVQAVRRKIMVSKRDHGRTDSLFKEIKEIDMELVSVKKICESVTDDNVTLQSKI
                     DIMEESNRTDSQVMGVVQSYIFPRRVPETDNYEAAFITLKQSDVSGDMYDFYTHERTL
                     KGVSLFETTSHGLAPGLVTILMRSIIYKRFIQLWNEKLSLIFEKVQKDLSTELGGMNI
                     FLSGKILKFDQERVEYVNAGHTDILFRSASNGVVRVVAPAGRSFKSQKMGGKSNVQDF
                     NMLSFSVKKGDIFLLFTDGFERCQNRSGAKYGLPGIIETFESIPEGRDIADVLDYFVK
                     NLNNHRDGASLDDDITIIVVRKL"
     gene            complement(7749..10859)
                     /locus_tag="P5123_04780"
     CDS             complement(7749..10859)
                     /locus_tag="P5123_04780"
                     /inference="COORDINATES: protein motif:HMM:NF024824.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HRX15605.1"
                     /translation="MSELKQYHYKSRSQRIIALSVAFLVNFAVGVAITMVFIDAKTVF
                     FYRSVFNNVVIYMLFFHFLGIIAGSQIFKRLKQSRWFFCGALLLFTLVFITLFSVKYF
                     LNDYYRHLIVAYIEHTPFLVLVVSAVPFVGGVINNYILKVVCGKFFDEKKSGHFFVMH
                     MLLGLIAGGVWFSAVQLDFLPGFLLFMPFLPLVALAFIFKLEYSPDPFFAREVKYSSE
                     IKKTETAVEKKEDLVFNYLNFLYIVIYTILGFFTFIRFYNDLLYIKLFFLSVIFIFLA
                     LGFMLSFVLKSRYWHIYSEMYYPFFFLFFFIMLNRFSDQLPFYYGALFIIPLAFCFGM
                     TLYHTTQFIIGRYDQIHAYRILRFGIFLIPIPVIIALSFVVFSNRLFFVFFYLVAALN
                     IVLPGLHLAQRSDNEYRKGLYLLLFIIVVPAFIMTHLYFKISLDSALYVTYTRNYDAV
                     YSFNSNSDYFDINSDILFNDKRVMKISDQFIRDLRRSVLSLTLFTDNNRDGVLFIDGN
                     RKFFDNNVYEIFDNGECLDYMLDSQVDYNSTPVSGRKSMVTFKSDIFTFFKNRDRRYD
                     AIVDIPNIYDQTHNSIRFSETYYEVVKRYLEGKSLFFQLIQPESASSCLVNSAVSGFK
                     KSFKHHIVFYFGENLVLAGSNESDFAINREGLERINYQISSSKKNRSLFYRDVHCLSY
                     ILDSSLPETKNVPQKIDYLSSPFMGDCNDVFSESGMLRSFDSIHDKFLSLKFDDDSMD
                     LKKTLAVILEDNRDILTLLKKSENVALKGDFKEEAEFLSSLRKAGEYDYDIRHYVEGL
                     LTVKELTFVRAATELEEGRNWTEAKDIYESILILNDSNFEAHYRLGLIYITLQQLDKA
                     FVHFDRALRLEPNNPQVLYQVGVLMYMVGEYRYALEYLRRAINYNLQHAMVFLYSGLA
                     NESLGKYRDARQDYEKALLADPNNPDIKASLSRIENRIESSVVDYSLPERRNQNEVEE
                     GEYIPLPINKNAIDKRLSEDELRFFSPRDKSEGSKYGDKYAEPPGAEKTEEPEPGNSF
                     FDLFGG"
     gene            11170..11478
                     /locus_tag="P5123_04785"
     CDS             11170..11478
                     /locus_tag="P5123_04785"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRX15606.1"
                     /translation="MNAQYKPIPEDNTFILMLLIIFSGGLYYLWWLSRVSKLFNDNPV
                     TNILLVFATLGLWALYLNIRYLQKSEEINGRSIQWYIIFFLPFAVLIIQNNINEHYLK
                     "
     gene            11538..14141
                     /locus_tag="P5123_04790"
     CDS             11538..14141
                     /locus_tag="P5123_04790"
                     /inference="COORDINATES: protein motif:HMM:NF025691.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4389 domain-containing protein"
                     /protein_id="HRX15607.1"
                     /translation="MIVKCSNCESAFTVSDEKVSGKKFAFSCPNCSHNNIIDNTGSEA
                     GSATSSASAESISAPATPSGAPIFDKQSPVSQKSSEASDFQDSDFSLDDNFDSDDSSD
                     FDDKDGLSDFDTTENNEDKLPSFDDEDDENNFSDFDLDDDSPTTSTNIDYDEMDYDIE
                     PEIDLSVLSPDNIPAENKSAPETSDDNDEITVDLDSLDIDFDDETDTDVRETSLPLED
                     DENEELSVDLDSLDIDLDDDTVKGDDFENEELNLTSDESFFETDSSIGEDDEDIPIDL
                     DNLDIDLEEDDDLVTSGPAFTLTEDESEDISLTSDTNDSFDLDDIDLESDEFDNDMTL
                     DISDDSSMDDDLSIDLENLDIDLDTSDAKQAAVKEQKDYETKEEYNSDDDDNDDDDEI
                     NIDLDSLDIELDESDMEPVDNDLMQLDDSLDDDLFDDIAGDNDDDQLSATPIALPADD
                     DDNDITIDLDKLNVDLDDDAAQLDTDSLDTERKIDSDETIDLDSLDADFEESDAFNTG
                     EDLFDEDESISIDLDTLDLGSETASVESDGEDESRLSLSDAGLTLDEIETESVKESFS
                     SNDLSLTDIDDDEDELHLTADEVNINLDDVTEEDESSSIFDPEPDNFDEFDSLEHIPA
                     DELPELDLDRFDEIVPEKPAASSETDIFDIGTYESGTNNDDGVVGPGYIQFSVDYSLR
                     YSRIKALLKFFGIYYILYIPHFIVLMLYMTVSSILTFINTVIVLFTSHTEKDFTAFQE
                     KTLRYGIGLYLSIFNITEELPHFAGATDIDHQLQLHAYPPVKFSRVLAFMRLSFIGIF
                     IITLPHILILSILSFAVLFFHLLGLVSVIVTGKWPAFVFDFMVRYFRYLSNICAYITG
                     IVDSYPSFRFD"
     gene            14134..>14623
                     /locus_tag="P5123_04795"
     CDS             14134..>14623
                     /locus_tag="P5123_04795"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRX15608.1"
                     /translation="MIDKYKSIITGIFDDNGDLASNLKDYRSRPSQIKMALQTADALF
                     KEKHVAIEAGTGIGKSLAYLVPSILYAVSEGKRGAISTETRTLQNQLVSKEIPLLQQI
                     MLPYTTRKIKIEICLGSGNYLCLRKYDHARASGVFNKHELKKIHLIDEAVENNNIITR
                     IDG"
BASE COUNT         4890 a         2972 c         2503 g         4258 t
ORIGIN      
        1 ggtttaaaga ggcatacact tctgccttgt tcaatagaga ccaccttaat aaccaatcag
       61 gatactagct cgttatactc ccgtcttaaa ccgttttcat gtcgccccat atatttcaag
      121 attatttttt tagaaggttt tttttgcaaa acttctaaaa gacgcaatac aagtagatat
      181 tttcttctcc aatgctgtac cgacggtata gatagcttaa gctctttggc aatctcccag
      241 tctttcataa ggtaattacc ttttttatct tttttgttaa gcgaacaaaa tttctcaatt
      301 acctctgact caaaagcttc tatgcggttt ttcttgacct ttctgtaatc atgttcactc
      361 caacgttttt gattacgctc ctgccaaccg gacatttttt tgtctttcca gtacggatga
      421 cggttacggg catactgaat atcttcgctg gtaaaaccgg tctcctcttt ccattgacgg
      481 caaagaattg tctttcgccc tctaggcaca tcactgtaaa gagatctgtg aatgtattca
      541 tccggtgtct ttgagttaag tatctgtttt tcagaaaaat tggttttttt gtttgatatt
      601 tgtttcatag accctctaaa aatatttttt tttaaaaaat ttcattactc gcataacgtc
      661 aaacattaat ttttatttgg tcttatttat taaaaaaaaa ttatatttaa ggtctaattt
      721 taatattttt ataacagacg atctttatgc aaccgtatcc ataaaacccc atcgtgcggt
      781 atatggcaat aaaaacatgt ctttactcat atttttatag tatattctat ttttcatatt
      841 atttatcccg ctaacattct acagagatta acataatgca attatatatt ccacacatta
      901 tagaacacgc gttattttga ttcctgtaaa acgcagaaaa caatataacc tgttacgata
      961 tgttaaaaaa aattcactac aatgctttgg taaaaccaag ttttagataa aaaggggcta
     1021 ccaataaggt gagaaactta aagaaggaaa cttcggaata tttgataaag atattctact
     1081 caaaggataa gtgaaacgga taaatctgaa gtgtcactca aaggagtttt catccaaagg
     1141 aaagtcgccc tgcttaactt cttgagaata gttatcaaga gcggttataa ccttacccgc
     1201 aagacccgca taatctttaa ccatcttggg acggaacgta tcatctaatc ccaagacatc
     1261 attaataaca agaacctgac catcacaatc tggccctgcc ccgattccta taacaggtat
     1321 tctcaatgct tcacagatgc gtttagccaa actacgaggc acaagctcca gaacaagtgc
     1381 gaaagctcca gcatcttcta ttcgcttcgc atcatcaacc attctccgag ctttgtcttc
     1441 ttctcgcccc tgaaccctga acccgccaaa attatgaatg gattgtggtg tcattcccag
     1501 atgtccacaa actggtatag aacaggctgt taaatgtgaa acaagttctg taaaataaga
     1561 gcctccttct agttttacgc tattggcctt gccttcctga ataagccttc cggcattctc
     1621 tatggtcttc tctttcgaaa cgtgatacga caaaaaagga agatccgcga cgatgaacat
     1681 attcggagca cctcgtctta cagcagctgt atggtaaacc atctcatcga ctgtaacagg
     1741 aagagtatta tcataaccag cttgcaccat accaccactg tcacctacca atataacatc
     1801 aatacctgaa cgtgacacga gttttgccat cgtataatcg tagcaggtta tcatcgatat
     1861 tttttcaccc tgctttttca tatcaagcat ggtattaaca gtaatgtttt ttcttttcat
     1921 tgtacacctc aatgtaagcc acaccgtcgc gtggatcaga taaatcaggg ttaagctcaa
     1981 gcaaatgctt aagtacaaaa atccgctcgc gaatgcccgg atgcggtaaa acaagatccg
     2041 ggcattcaac aacagtattg ttataaagta gaatatcaag atcaattaac cgcggccctt
     2101 tttcaacaga aggcacacga cctaatttaa cttctatccc tttcaatttt actaaaagtt
     2161 cattagcaga gcattcggca gatacataaa caacagtgtt taaaaaatcg ggttggttct
     2221 catacaaaac cggttttgtg ttttcgatac tgctttgacg caaaacagtg ataccacaac
     2281 ggttcatctc ctcacaagct cgaattatgt taagttctcg attgcccaga ttcgtaccaa
     2341 tgccaatata tgctttattc atcatttctc ttcacggtga tctgaacctg aaagttccat
     2401 tatataacac atctctttta tccgagaaag cactcggcct ccccctaact ctttcatgga
     2461 ctcggccgga ttgttattag aggtgaaaat agtaaatttt tcggcctcat agcgtgaatc
     2521 cacaagatta tagagcgtct cctgttccca aggtgaatca cgttgaacac caaaatcatc
     2581 gactaccagc acatcaatct cggcaagatc acgctcgatc tgcgaggact gaccgtacat
     2641 ttgactgcct tcaacgaaag tagagcgtaa gcgtccaaaa aaattacggg aaattttaag
     2701 atactttcca gacaccgcgt atctgctaat tagttctgta agaatgatag ccgatatcat
     2761 tgtcttacct gttccagggt tgccccacaa aaaaagtccc ttcgaaacat cgggaaagct
     2821 gtgaattatt ttatatgctg ccgattttac tttgttatcc gcctgtgttt ttacattaaa
     2881 atcactcaaa aatagccatc tgtacttttt atcaatttca gatttcgaaa acagtgcatt
     2941 aatacggtta attcgacgtc taaccggatt acaagggcac tcactgattt tcccattttc
     3001 ggcataaaga taggggctct ctccaccgca cctgcaatgt ggtaaaacac atttagggca
     3061 aggagttatc ggattttcac ccgtaatata ggcatcagga ttcttgataa ttcctgtgcc
     3121 ttcacaaaac tgacaaaact gtacaacttt tgttttctct actggatcct gacccataat
     3181 accgccggtt ataaagatat taataatgac aattacttct cgcaggtttt ttcacataaa
     3241 atatctgtca attattaaat gctaaacatt ttgatgtaaa aatccgaaat ttgtagtagc
     3301 tttattgcgg gcgcgtgtta attttagata caaataatta cgtcggcaac agcagataat
     3361 aatgagcaaa tataatagaa gaggaatccc tatgaaacgt aaactgtgta tgactatatg
     3421 cgcactttct gtcctgaccg ttttaatctt tgcatccagc atttacgcgc aagatactgc
     3481 cgcgcccgcg gttgatggcg acaaagtgcc ttctgaaatg ggtggacagt ggcgagttga
     3541 ggattttcac ccttacgtaa aagcacttga tgacctgaga aaactttcga aagagtatgc
     3601 tgaaaaaata atgataaaat ctatcgatga atactctatc ggaatggata tactacgaga
     3661 tatggacgca aacctctcta accttcaaaa acagtatgac cggcaaaaca atcttaatga
     3721 aagatggtat tggcaagaaa tagaccgtga gaaccaactc aagcgccgca aaggtatgct
     3781 gaaatattca gctaaacttc gcgctattac atattttaca cgcagtataa acagccttga
     3841 ccagatacaa tccgaagaag tcagaaaaga tccgaagttt gtggagtttc aaattcgcct
     3901 atttaaagca tacgtttcgg ctcaatacga cattcataac cttaagccat gtattcctat
     3961 ccttgaacgt tatattgcaa taaacgacaa tacaaaacag gacgtacaag catataaata
     4021 cttggcaagc tgttacggat atatggaaaa atctttggcc aaatataaca caactataag
     4081 tgaagagcgc gtaataaaat acaaacagaa gaaaaatcag gctctactta tggcaaccga
     4141 aattgagtac ggtattgaat ccatcgaata cagaaagctt aaagatatcg tagaaataga
     4201 tgaaatacaa tcagaaagaa tcaataattt tagatagaag ccgataatca gattttgcaa
     4261 ccattgagta ttaattttcc cgaatgaccg ctatcgcagc ggtcattttt tttcctcgtt
     4321 aaaaagcccg caataaatta ttaaatacga tattttttta tagcaaagac ttaagtcgtc
     4381 tggttttttc catgcaatca ttttgatctt ttcctccaac aaaaaagaga agacgtcgag
     4441 atcggtacag catgttttta taataaaacc tggggggtgt caaaagcctt acattgcgat
     4501 tatcaaacat ttccgctaat ttttggcaaa gttcgggtga atatactttt tgttgaatac
     4561 tgactatgcc tccacaatca actctaaacc gttttttgca aagtattgcc aaatcggcaa
     4621 tagaacggcg agcattaatc tcacaacaaa tctctttata tatgctatta ttagacttat
     4681 ataaaaaacc atcacaatta accggaccaa aatatcccga ttcgtatagt accttagaga
     4741 gcccaaaagc tgatttctcg attaagccat ataatgggct gtcacatgaa ttacagtaat
     4801 aaaagaccga actaaaaatt ccacggggac tgttatcaag agaacgtaca gttaaaaaag
     4861 acatttctcc ttctttcgat atataaaaac cgtaagaaaa atcactgact ctttttaccc
     4921 actgctcaca gacagcccta ttatcgatta caatcttctc cagacatctt tctgtctgaa
     4981 caatattaaa tccatacgcg gaacttccac aaagaggttt taccacaagg ggataatcgt
     5041 aatttttagc aaaaacacaa acatctttaa tggagctcac aatttcacgc cttattagtg
     5101 aacccatttt tttagacact tcaagagaaa aactgcgaca gttagcttta gctacattta
     5161 taacgccagg ataactttca gggtcacata ccaacgaaag gctatcgctg tttattcccc
     5221 ataaatacaa ttcggaagat agatactgtt tgcactcctc ccgtatcacg agggatccgc
     5281 agtcacctga aatttgagca agatacttgt aataattctc ctcaggcctt acatgcgata
     5341 caattacatc atcatcctct gccaaagcaa gtggaagcaa gttcaaaaga taaacttggg
     5401 caagaaaccc ttctggaaca cgaacattgg ctatatcaaa atcagtattt aaataatata
     5461 tcatggcctt ttattttgat aacctttagg aatgttaaat aatttaaagc tttataaaaa
     5521 ttatttttat agctttcaac cggcaaacag gaaagttgca aattattttt cactaggcga
     5581 tgttaataat ctagagcttt cttactacta taattgtaat gtcatcatcc aaagaggcgc
     5641 cgtccctgtg attattcaga ttttttacga aataatccaa tacatccgcg atgtccctgc
     5701 cctctggtat actctcaaag gtttcgatta ttcctggcaa accatacttc gcaccacttc
     5761 tattttggca cctttcaaaa ccatctgtga aaagcaaaaa aatgtctccc tttttaactg
     5821 aaaaagagag catattaaaa tcctgaacat tacttttgcc acccattttt tgagacttaa
     5881 agcttcgtcc ggctggagca acaacgcgta ctacaccgtt ggaggcacta cgaaaaagta
     5941 tatccgtgtg tccggcatta acatattcca cccgttcctg atcaaatttt agaatttttc
     6001 ccgacaagaa gatattcatt cctccaagtt ctgtgctcaa atctttttgc accttctcaa
     6061 aaattaaaga gagtttttca ttccatagct gaataaaccg cttgtatatt atcgagcgca
     6121 taagaattgt tacaagaccc ggcgccaatc catgagatgt tgtttcaaaa agcgatactc
     6181 cctttaatgt gcgttcatgg gtataaaaat cgtacatgtc accgcttaca tcactctgtt
     6241 ttaaagtaat aaatgccgcc tcgtaattat cggtttccgg aactcttctt ggaaagatat
     6301 aactctgaac aacccccatt acctgtgaat cggtccgatt actctcttcc atgatatcaa
     6361 tcttagattg aagagttaca ttatcatctg taacagattc acatattttt ttaaccgaaa
     6421 caagttccat atctatctct tttatctctt taaaaagtga atcggttcgt ccatgatcgc
     6481 gtttactcac cattatttta cggcgtactg cctgaaccga agaagcaatt gtaataatta
     6541 cgcttccaag cccaataaaa acaaccggcc ttaaaagatg ctcggaagcg acaaaccata
     6601 ttagaccgac aaagagcaat aaaacaagaa gtagggtctg catctgatag ttaaaaaaaa
     6661 tgcaaaatgg gaatattcgt tcaaaaaaag tttcagcaag tggactagca aaaaagttct
     6721 taataacatt ataattaaaa agaagagaaa ccaaagctat aagcagtaga gaaacagaaa
     6781 aaattaaccg gttaaaacca aaaaaataga gtagaataac aacagcaata aaccctagta
     6841 acaaatagca ggcaatccaa agcgaataac gattgccccc acgaaaagag atcaataaaa
     6901 ttgagcaaag tgaaataaaa gagagagcgc cgtacaaaac aggcaaatca aatcggctaa
     6961 tgttaaaaat aagatataaa tagtgggaat cggccaacaa aagagccaaa taagccatca
     7021 tgatacctat agaaagtgcg atgcatcttc gatcagaagg cgaaatcaat aaaaacaaga
     7081 gaagtagacc tgtcgcgaag acaataacgg cagctaaaat tgctatttta agatgaaaat
     7141 catactcata ctgctgcaat ttcgcaaaaa aatctatcga aaaagggaag ccctcggtaa
     7201 taattcctga aaccggatct tcattaccac ccttgacccg gaaacagagt acattaaaac
     7261 cgtcatttaa cagatagaaa ggtatttcaa taaccttatt acggaaaatc aatgttgtat
     7321 caatatcaaa atgattatac agtgattttc catttaaata tagttcgaaa ccagagccaa
     7381 tcagaggtat agaaattccg agagatcttc taatcgacgt atgacgatct atatgaaaac
     7441 tggtaagtaa cgtaaactgt ctatcatcaa tttctgcttc atccttgaaa agctccggat
     7501 aagttaaagc tctatcataa cctgctgtta ctattttttt ccaatcaact gagggcggaa
     7561 aggttgatat ctgttcggga taaaagccct ggcgaacata aaaatctcta ttggtaagat
     7621 caataggaag gttttgaccc tgcataggta cactgagcag aaccagacac aagggaacaa
     7681 gagctttcaa aacagctcta tatagcatga ttaacctgac ccaacattat aaatccgaca
     7741 atcatggatt atcctccaaa aagatcaaaa aaactattac ccggctccgg ttcttcagtt
     7801 ttttctgctc ctggaggttc cgcatactta tcaccgtatt tactgccttc tgacttgtct
     7861 cttggcgaga agaatcgcaa ctcgtcttct gacagtcttt tgtcgattgc atttttatta
     7921 ataggtaaag gtatgtattc accctcttca acttcatttt gattacgtct ttcgggtaat
     7981 gaataatcga caacactgct ctctatcctg ttttcaatac gactcaaaga agccttgata
     8041 tcaggattat ttggatcagc aagcagagct ttttcgtagt cctgcctggc atctcgatat
     8101 ttgccaagac tttcgttagc caaaccgcta taaaggaaaa ccatagcgtg ttgtaaatta
     8161 tagtttatgg cgcgtcttag gtattcaaga gcataccggt actcaccaac catatacatc
     8221 agaacaccaa cctggtatag cacctgagga ttattaggct caagccttaa cgccctatca
     8281 aaatgaacaa atgccttatc aagctgctgt agggttatat atatcaaacc caagcgataa
     8341 tgagcctcaa aattgctgtc atttaaaatc agtatgctct cataaatgtc cttggcttct
     8401 gtccaatttc gaccttcctc aagttcagtc gcggctcgca cgaaggtaag ttcttttaca
     8461 gtcaacaatc cttcaacata atgcctgata tcatagtcat attcacctgc ttttctcagg
     8521 ctagacaaaa attcggcttc ttctttaaaa tcacctttca aagctacatt ctctgacttt
     8581 ttcaataatg taagaatgtc acgattatcc tcaagaatta cagcaagtgt tttctttaaa
     8641 tccatactgt catcatcaaa ttttagagaa agaaacttgt catgaatcga atcaaaagaa
     8701 cgcaacatgc cactctcaga gaaaacatcg ttacaatcac ccataaacgg tgacgacaga
     8761 tagtcaattt tttgaggaac attttttgtt tccggcaaag aagagtccag tatataggag
     8821 agacagtgaa catctctata aaaaagtgac cgattttttt tcgagctaga tatctggtaa
     8881 ttgattctct ctagcccttc cctgtttatg gcaaaatcac tttcgttgct accggcaaga
     8941 acaaggtttt ccccaaaata aaaaacgata tgatgtttaa aagacttttt aaacccagaa
     9001 acagcactat taactagaca agaacttgca ctttcgggtt gtatcaactg gaaaaagaga
     9061 cttttacctt ctaggtaacg ttttactacc tcgtaatatg tttcagaaaa acgaatacta
     9121 ttatgggtct ggtcatagat attgggaata tcgactatcg cgtcgtacct tctatcccgg
     9181 tttttaaaaa aggtaaaaat atctgatttg aaagtgacca tgcttttacg tcccgaaaca
     9241 ggtgtcgaat tataatcgac ctgtgaatca agcatataat caagacattc accattatca
     9301 aaaatctcat atacgttatt atcaaaaaat ttacggttgc catcaataaa caaaactcca
     9361 tctcggttgt tatcggtaaa gagagtcaag gaaagaactg agcggcgcaa atcgcgtata
     9421 aactgatcag atatcttcat tacacgctta tcgttaaaca atatgtcaga attgatgtca
     9481 aagtaatccg aattcgaatt aaaactgtaa accgcatcgt aattacgtgt ataggtgaca
     9541 taaagagccg aatcaagact tattttaaaa tagagatgtg tcataataaa agctggaaca
     9601 actataataa acaataaaag gtaaagccct tttcgatatt cattatcgct cctctgtgcc
     9661 aagtgaagcc caggaagcac tatattcaat gccgcaacga gataaaaaaa tacaaagaac
     9721 agcctgtttg aaaacactac gaaagagagt gctataatca ctggtatagg gataaggaaa
     9781 ataccgaaac gtaatattct ataggcatgt atctgatcgt atcgtccaat aataaactgg
     9841 gtagtatggt atagagtcat tccgaaacag aaagccaaag gaataataaa aagagcaccg
     9901 taataaaacg gcagctgatc ggaaaatcga tttagcataa tgaaaaagaa cagaaaaaag
     9961 aacgggtaat acatttccga gtaaatatgc caatacctgc tcttaagaac aaaagaaagc
    10021 atgaaaccta aggccaagaa aatgaatatt accgataaaa agaacagttt tatatacaat
    10081 agatcgttat aaaaacgaat aaaagtaaaa aatccaagta ttgtgtatat cactatatac
    10141 agaaaattca gataattgaa taccagatcc tctttctttt ctacagcggt ttctgttttt
    10201 tttatttcac tgctgtactt gacctcgcgg gcaaagaatg gatcagggct gtactccagt
    10261 ttaaaaataa aagctaaagc taccaacggc agaaaaggca taaacagcaa aaatccgggt
    10321 aaaaaatcca gttgtacagc agaaaaccat actcctccag caataaggcc cagaagcata
    10381 tgcattacaa agaaatgtcc gctctttttt tcatcaaaga acttgccaca caccactttc
    10441 aaaatatagt tatttattac tccacccaca aaaggaacgg ctgaaacaac cagcacaaga
    10501 aagggtgtat gctcgatata tgcaacaata agatgtcggt aataatcatt aagaaaatat
    10561 ttaaccgaaa acaaggttat aaataccagc gtaaacagaa gaagcgcacc gcaaaaaaac
    10621 caacgactct gttttaaacg cttaaaaatc tgtgaacctg caataatacc caaaaaatgg
    10681 aaaaaaagca tatatattac tacattatta aagacagaac gataaaaaaa taccgttttg
    10741 gcgtcaataa acaccatagt aattgccaca cctacagcaa aattgaccaa aaaagcgacg
    10801 gataaagcta ttattctctg agaacggctt ttataatgat attgtttgag ttcactcata
    10861 gattattccc ggaaacagta cttaacaggt tatatatgtt tttcttaagc accgtattgt
    10921 tattcttttc ctgtatatta ttgacgcttt tatgacaaca attttttatg gagaatattt
    10981 tcttatgcaa tatggtattt ttaagctatt agaaacagat aattatattt attatttaac
    11041 caaattaaaa atttcaccga tactatagac gagtagcttt tttatagttc taggtatatt
    11101 cattacaact gttttatttg tacttaaact atcagaatac tactgcaata aagccctatt
    11161 ggagcgcata tgaacgcaca atacaaacca atacccgaag ataatacttt tattcttatg
    11221 ttactaatta ttttcagtgg aggtttatat tatttatggt ggttatcaag agtaagcaag
    11281 ctctttaacg ataacccggt aaccaatatt cttctggttt tcgcgacact ggggttgtgg
    11341 gctttgtatc ttaatatcag atatttacaa aaatcagaag aaataaacgg ccgatcaata
    11401 caatggtata ttattttctt tttacccttt gctgtgttga tcattcagaa caatattaat
    11461 gagcattatc ttaagtaaac tggtgtttac aaacagccaa tgacgatatc tatcaactat
    11521 ccatgcggga gtacaatatg attgttaaat gcagtaattg tgaatcagcc tttactgtca
    11581 gcgatgaaaa agtaagcggc aaaaaatttg ccttcagctg tccaaactgt tcacataaca
    11641 atattataga caatactggt tctgaagctg gctccgctac atcatcggcg tccgccgaat
    11701 caatttctgc gccggcaact ccctctggtg caccgatatt tgataagcaa tcccccgttt
    11761 ctcagaaatc ttctgaagct tcggactttc aagacagtga tttttccctt gatgataatt
    11821 ttgattctga tgattcatca gatttcgatg ataaagacgg actttccgat tttgatacta
    11881 ctgaaaacaa cgaagataaa cttcccagtt ttgatgatga agatgacgag aacaattttt
    11941 cggactttga tctggacgat gactcgccaa ctacttccac gaatatcgat tatgacgaaa
    12001 tggattacga tatcgaacca gaaatcgatc tctcggtctt gtcccctgac aatatcccgg
    12061 ccgaaaacaa atcagcacca gagacctccg atgataacga tgaaattaca gtagacctag
    12121 actcgcttga tatcgatttc gatgatgaaa cagacacaga tgttcgagag acctctttgc
    12181 cgcttgaaga cgatgagaat gaagaactct cagttgactt ggacagtctt gatattgacc
    12241 ttgatgacga cacggttaaa ggcgacgatt ttgaaaatga agagttaaac ttaacgagtg
    12301 acgaatcttt ttttgaaact gatagttcaa tcggtgaaga cgatgaagat attccgattg
    12361 accttgataa cctggacatt gatcttgaag aagatgatga tcttgtcaca tcaggccctg
    12421 cttttactct aaccgaagat gaatctgagg atatttctct aacatctgac acaaacgact
    12481 ccttcgatct ggatgatatt gatcttgaat ctgacgaatt tgacaatgac atgacattgg
    12541 atatatccga tgatagttct atggacgatg acctctcaat tgatttggaa aatctggata
    12601 ttgacctgga tacttcagac gcaaagcagg ctgctgttaa agaacaaaaa gattatgaaa
    12661 caaaagaaga gtataacagt gatgatgatg ataatgatga tgatgacgag atcaatatcg
    12721 atctggattc actcgatata gaacttgacg aatctgatat ggaacctgtt gacaatgatc
    12781 tgatgcaact tgacgatagt ctggacgacg atctttttga cgatatagct ggagataatg
    12841 atgatgatca actttccgct acaccaatag ctctgcctgc cgatgatgac gacaacgata
    12901 taacaattga cctggacaaa ctaaatgtcg atcttgacga tgacgcagcg caacttgaca
    12961 cagacagtct tgatactgaa agaaaaatag actctgatga gacgatagat ctggatagcc
    13021 ttgatgccga ctttgaagag agtgacgcat ttaatacagg cgaagacctt tttgatgaag
    13081 acgaaagcat ttcaattgat ctggacaccc ttgaccttgg ctctgaaacc gcatctgtcg
    13141 aatctgatgg agaagacgaa tctcgactat cattatccga tgcaggtcta acactggatg
    13201 aaatcgaaac cgaaagtgtc aaagaatctt ttagcagtaa tgatctctct ttaacagata
    13261 ttgatgacga tgaagacgaa cttcatctaa cagcagacga ggtaaatatt aaccttgacg
    13321 atgtcactga agaagatgaa agcagctcaa tttttgatcc tgaaccagat aactttgacg
    13381 agtttgattc acttgaacac ataccagccg acgagctgcc agagcttgac cttgatcgct
    13441 ttgacgaaat agtccccgaa aaaccagcag ccagttcgga aacagacatt tttgatattg
    13501 gcacctacga atcaggtaca aacaacgatg atggtgtagt aggacccgga tatatacagt
    13561 tttccgttga ctattctcta cgctattcaa gaattaaagc tcttctaaag ttctttggta
    13621 tatattatat tttatatatc ccccacttta ttgtacttat gctctatatg actgtgtcgt
    13681 cgatactgac attcataaac actgttatag tacttttcac ttcgcataca gaaaaagatt
    13741 ttactgcttt tcaagagaag actctccgtt acggaattgg tctttatctg tcaattttca
    13801 acattacgga agaactcccc cacttcgcgg gagcaacgga tattgatcac cagttacagc
    13861 ttcacgctta ccctccggta aaattttcaa gggtattggc gtttatgagg ttatctttta
    13921 taggtatatt tattataaca ctgccgcaca tattaatatt atcaatactt tcatttgcgg
    13981 ttcttttttt ccatcttctg ggtctggttt ctgtaattgt tacgggcaaa tggccggcct
    14041 ttgtttttga cttcatggtg cgctatttcc gatatctatc aaacatctgt gcttatatta
    14101 ccggtattgt agacagctat ccgagtttca ggtttgattg acaaatacaa aagcataatc
    14161 accgggatct ttgatgataa cggtgatttg gcatccaacc ttaaagatta ccgttcaagg
    14221 ccgtcccaga taaagatggc gcttcaaact gcggatgctc tttttaaaga aaaacatgta
    14281 gcaatcgaag caggtacagg tattgggaaa tctcttgcct acctggttcc ttcaatttta
    14341 tacgcggtat ccgaaggcaa acgaggtgcc atttcaacag aaacccgcac acttcaaaac
    14401 cagcttgttt caaaagagat tccgcttctt caacaaataa tgcttcccta cactacgcgc
    14461 aaaatcaaga tagaaatctg tcttggcagc ggtaactacc tatgtttaag aaaatatgat
    14521 catgccagag cttccggagt ctttaacaaa catgaactga aaaaaattca cctcattgac
    14581 gaagcggttg aaaacaataa tattataacc cgaattgacg gca
//
LOCUS       DAOTGZ010000047        14533 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_3064
            NODE_4981_length_14533_cov_5.004559, whole genome shotgun sequence.
ACCESSION   DAOTGZ010000047 DAOTGZ010000000
VERSION     DAOTGZ010000047.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868088
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 14533)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 14533)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:22:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,785
            CDSs (total)                      :: 2,751
            Genes (coding)                    :: 2,732
            CDSs (with protein)               :: 2,732
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 12 of 19
            Pseudo Genes (internal stop)      :: 5 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14533
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4981_length_14533_cov_5.004559"
                     /isolate="AMR_MDS_3064"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..655
                     /locus_tag="P5123_04800"
     CDS             <1..655
                     /locus_tag="P5123_04800"
                     /inference="COORDINATES: protein motif:HMM:NF012497.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="3-phosphoshikimate 1-carboxyvinyltransferase"
                     /protein_id="HRX15609.1"
                     /translation="HFGINIINKDYKVFEIEHGGRHDLEKYNVEGDWSGAAFIMVAAA
                     LAGTVTINNLSSQSSQGDKEILTIIKNSGAQISENRDSITVHRSILKPFNCDATDCPD
                     LFPPLCALAAGCNGKSRISGIHRLAYKESDRAAVLIEQFKKIGVGIQIEGNDMIINGG
                     QVAGGVAESCNDHRIAMALAVAGLISDKGVTIENAEAVQKSYPVFFEDLHDLGAEVQ"
     gene            652..1632
                     /locus_tag="P5123_04805"
     CDS             652..1632
                     /locus_tag="P5123_04805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018612471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate synthase"
                     /protein_id="HRX15610.1"
                     /translation="MNSFGNIFRVSLFGESHGSAVGAVLDNVPPGIPLSSEDFVLDMN
                     RRKSGKKGTTPRLESDIPDIVSGVHEGLTTGAPIMIMIKNENTRSKDYSLFREMPRPG
                     HSDFTAQKKYGGFNDLRGGGMFSARLTTAIVAAGVVAKKICSPVNYKAEIVHIGGNID
                     IEAELDSVMKDKDSIGGLVKCTGINMPIGLGEPFFGKFEAQLAHIMFSLPAVKAFEIG
                     SGVKAALMRGSEHNDCFSDENGKTLTNNSGGTLGGITNGNSIEFSVTFKPTASISIPQ
                     KTFNFEKGKIDTLIIEGRHDACLTLRTPVIVEACAAIVTADLLLYNRIYG"
     gene            1645..2697
                     /gene="pheA"
                     /locus_tag="P5123_04810"
     CDS             1645..2697
                     /gene="pheA"
                     /locus_tag="P5123_04810"
                     /EC_number="4.2.1.51"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020263847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004664 - prephenate dehydratase activity
                     [Evidence IEA];
                     GO_process: GO:0009094 - L-phenylalanine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prephenate dehydratase"
                     /protein_id="HRX15611.1"
                     /translation="MILKDIRKKIDLKDAQILKLLHERMELAVMSKKFKNKIEDSERE
                     EELFKMIDAHSGVLINPEFSKKLYKDIISESKRLQGKNYKLIGFQGEQGAYQELASLV
                     WDKNLIPTACSSFAEVFNGVETGLYDYGIVPIENTLGGVVNQTNDLMIHSDLFVVGAV
                     ELEIHHCLLALPGTDHRDIRMVYSHQQALAQCRNFLARHNLEGIPYYNTAGAAKMLMH
                     EKPKASAVIASRHAADIYNLEIIKENIEDLDTNRTRFLIISKKESSEKGEKCSVLFST
                     AHKAGTLFHVIEIFAKENINLTRIESIPNRPGTYVFFLDFEGDKNDPRVQNVLKGVSE
                     VTGEFRFMGCYREVHA"
     gene            2713..3468
                     /locus_tag="P5123_04815"
     CDS             2713..3468
                     /locus_tag="P5123_04815"
                     /inference="COORDINATES: protein motif:HMM:NF022112.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GerMN domain-containing protein"
                     /protein_id="HRX15612.1"
                     /translation="MSSEKVSKKLSKKRITKKTPEKRGKAQKPVPVSGGKNAVLILIV
                     MALVTVLVFMGVQMLKLKNAPHTRVEKSIVEPDKSLTDSKKSKDEGKKKEKPDDGSTV
                     AGETSTDKAKKPDLITGNIYLLFFDERSELITLRPVARRFRSDQLLTDAFSMLVKGPL
                     KEEKNKGFETSLSPSMRFNTIKVKNKIAYIDINPDFVKDSFGDIGVARVNQVFLTLTQ
                     FDSINGIVITVNGKKIDTMDDGRHFVWPMTRRL"
     gene            3468..3980
                     /gene="cyaB"
                     /locus_tag="P5123_04820"
     CDS             3468..3980
                     /gene="cyaB"
                     /locus_tag="P5123_04820"
                     /inference="COORDINATES: protein motif:HMM:TIGR00318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class IV adenylate cyclase"
                     /protein_id="HRX15613.1"
                     /translation="MYEIEIKAYCNDLSLIENELELEKALFVGEYKQHDIYYNHPLRD
                     FKETDEAFRIRSVDGNQFVTYKGPKISQTAKTRIELETSIGEFESFRSILLSLGFKEG
                     GEVIKIRKEYILNNVTICLDNVYGAGSFVEFEIMGSDIAKGEQALFEMAEHLGLRRFE
                     RKSYLELVLG"
     gene            4010..4138
                     /locus_tag="P5123_04825"
     CDS             4010..4138
                     /locus_tag="P5123_04825"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRX15614.1"
                     /translation="MEQKVYIYICDHCKKMSRVNKDDPIPHCCGQLMRRYDDANNK"
     gene            4503..6092
                     /locus_tag="P5123_04830"
     CDS             4503..6092
                     /locus_tag="P5123_04830"
                     /inference="COORDINATES: protein motif:HMM:NF024484.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3943 domain-containing protein"
                     /protein_id="HRX15615.1"
                     /translation="MKKNIINNKKAILKLVIVIIAITHIYSCQDGHDYIFSESDIRKE
                     SNETKTFDNKSENMSDDIETDDEFNNSISSTLATNFTNTMYGVGSILGSGTVWYYLEK
                     SNKRDQFFNSGDDIRTRFTSFDAWRFDDNLFETNAVSHPMAGAGYFLSARLNNYNQYE
                     SFLFSLGGSLVWEYFCEFQEVVSINDMIFTPVGGYAIGESFFQLVNFYADNSSNSSLG
                     DTIQYYTKSQDGRYKNGLRNSQGNNPFWYNAYLYGGYSQTDSEHYTTHFGLYTQFYGT
                     RGVKENGYYNKFLYNSPYTKIEIDFGAGNTWLKDCYLFFESGHLSYVNQNLHNEKAGY
                     AFIATLTTAFEYRNYYYQGFRDRIGAVSPVKLNTDFLFDYKDFSINLELGLSPNFAQV
                     SPFGLGLFYQDGYHSDYLREHGAHTTANRGYYFGYGFSSKNSLTISYKKYQSGLSYNF
                     DYYKSINTDGLTNREEPTNKNFKMKDERSVFSSWLKYDITDAWSLVAKYYRNEIEGTV
                     EDYSRSDFERSIKLLTLYNFR"
     gene            complement(6095..7000)
                     /locus_tag="P5123_04835"
     CDS             complement(6095..7000)
                     /locus_tag="P5123_04835"
                     /inference="COORDINATES: protein motif:HMM:NF012376.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HRX15616.1"
                     /translation="MKTNFFYLLLLTTFFSCSTELIINDNVEPSETLIIWAHSDIQPR
                     YLWEKYHYEYAIDDVRSLPFVPDMAIVAGDINHRQSSDMYWNWIKDLRKKTGITYWFE
                     IAGNHDNHDIETYKRQSGKPLHYTVRCGNLLFIFLSDETKKANTIISDDVFEWWKNIV
                     INNQDSIIITVTHAPLFQSQLVSTLNWTMIIKDSDRFWDVIKDYTVDVWISAHDHLPS
                     MIPIKYNTPDNCKTLFLDVSSIHKVRFSPIESWFLIFQNGSSSLQCAARSHEKKLFYS
                     SSFTYELSKKVFYKRNPPTIDKKYQ"
     gene            complement(7077..8132)
                     /locus_tag="P5123_04840"
     CDS             complement(7077..8132)
                     /locus_tag="P5123_04840"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRX15617.1"
                     /translation="MSKLTSADLKQITEIRKQISAAEKSNNQNDSKTLSEQKKELQIL
                     KKKAMQVLSGTEKRINEIQRDLKPLVKVISQLTADANNGNHDSKKAASVKIKDSQKKL
                     QELQKELELRKKQQRAIIQALNDKPASKPKNTFSISNKKTPPTKKEKKGTDTKISQKF
                     VFNPLQIFKSKPYIPVAIIIVILLTGMIAIYFYNKGTPSIEAPIESTEPGKAQIDDSI
                     IEINAKGTITGNNVNLRVQPDVSSDVIRQLHNNSKVNVIAKTNSANRNGAVLVGQTQI
                     TMESGKKIKLDAGKGITITDESESSYTVSYPISDGKIISGTVDKNSVKKIAGETWYQV
                     KTSNTTGWIFGKFIIVE"
     gene            complement(8392..9528)
                     /locus_tag="P5123_04845"
     CDS             complement(8392..9528)
                     /locus_tag="P5123_04845"
                     /inference="COORDINATES: protein motif:HMM:NF019522.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YibE/F family protein"
                     /protein_id="HRX15618.1"
                     /translation="MNRSGIFLFLSIILICFATNISAQDFDQEITYAKAKINTIETRR
                     LTDETTGEVQIETIFSLEILSGDFKGETKQVVYAGQDSMPAYSRYKEGDKIFIGFNSI
                     MMEDETDDYIALYDLDNTVGITIVMILLVITIVGIGKTKGLFSLLALMITVGLIFFVF
                     VPLTVKGYPSLVLTVLISIISIFITIPVITGITRKTTAAITGATSGVIVATILALICG
                     WSMHLAGIITDDMIQVFYLAENRIDLRNLALSGMIIAALGAIMDVAVSIASATSEIFE
                     ANPKIEFKKAFVSSLTIGKDILGSMVNTLILAYVGSSLSIVLIIYMKYDAQMPISMIF
                     SHNPVLIEIIKSFVGSLGMFTAIPATAFIATKIHFDKYNKATKK"
     gene            9869..11275
                     /locus_tag="P5123_04850"
     CDS             9869..11275
                     /locus_tag="P5123_04850"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRX15619.1"
                     /translation="MAEKNILSILIAAYLPDHNETLLFAGFFDTIRIQLLLYFNTVIV
                     SSEIQSTVKDLDIDEFVHFIFEAKDNYTIDTDLNNRATDYFVGEFNAKTIESIITKHS
                     QNDTGALSFNFKRMIDHLLQLDTTQIQMKICMTSHGALEIFNAYERRISHTVSENNEI
                     ASKYVGSNRDLIKKIVESSIADSTVAVFTVSKEGKIVSCGSVIYNELSNKLVHKNYIY
                     AQGISFDECNLANNLVDVFHYIQEFSENCTNNDEVTNKIDNAVSKINIDRLAVIMKSA
                     DSDKVNSFFSRIMPPEITSGNLEIRVQKFNSIILDIAFEPVLLGQKSKEKALKEETVP
                     KSKAQVEVDLVISPTKGVKVSELKSGSRIYVIINGTTIIGRKVINQLNLLDENRVKPV
                     SAVVEEITKTSDGYIVITRIASNLLGRAIEEEDVKVKSGDPVVDKKIEKSRKSLLVGV
                     SVGLGFILLAAISLWVLL"
     gene            11522..11998
                     /locus_tag="P5123_04855"
     CDS             11522..11998
                     /locus_tag="P5123_04855"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRX15620.1"
                     /translation="MPYNKVAGEDSSAHDSGPTEKYYRLLHLYYLWTTNFYRGDNYYH
                     REIGSTLYSYINSNFETPRILGRIEQLHGQGGAAYSCFRFSSWLTIKFDLREKAVVEL
                     LSSLTQRERALGIDIGISGTIYRYKVINREKDRSIEIYLRDVNIIKDKTKAPPDES"
     gene            12169..14007
                     /gene="glmS"
                     /locus_tag="P5123_04860"
     CDS             12169..14007
                     /gene="glmS"
                     /locus_tag="P5123_04860"
                     /EC_number="2.6.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020251425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004360 - glutamine-fructose-6-phosphate
                     transaminase (isomerizing) activity [Evidence IEA];
                     GO_process: GO:0006040 - amino sugar metabolic process
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine--fructose-6-phosphate transaminase
                     (isomerizing)"
                     /protein_id="HRX15621.1"
                     /translation="MCGIVGYIGTKAATSVLLKGLKCLEYRGYDSSGIALLNDSLFVC
                     KKKGKIIELEKELNGLSKDLSTYTVGIAHTRWATHGEPSDLNAHPHVSSSGKIAVVHN
                     GIIENFQILREKLALKGFKITTQTDSEIIAHLIEYYVDKYNDFLTGVFVALEKLEGTY
                     GIVVISRDYPDRIIAARNGSPLILGVGEGEIIIASDASAIVEYTRKVIYIDDREVVDI
                     QRDSYQTYDLLQKQVEKPVEDIDWDIETIGKKGYDHFMLKEIFEQVESVKNTCRGRLL
                     TETGTSRLDGLNLKPEDFSEIKRIILIACGTSWHSALIGEYLIEEYARIPVDVEYASE
                     FRYKNPILTKSDLVIVISQSGETADTLAALREAKQKGVKVLGVTNIVGSTIARESDGG
                     VYLHAGPEIGVASTKAFSSQVMVMIMIAIHFARRKDMSLESGQVLIEKIEKLPQLIQS
                     MLDTAEDIKNIAEIYKNASNFLYLGRGVQFPVALEGALKLKEISYIHAEGYPAAEMKH
                     GPIALIDENLPVVFIALKDSIYGKIVSNMQEVRARGGKIIAIATEGDNEISNYADHVI
                     FVPDVDRMLSPILTVVPLQLLSYYIAVMRGCNVDQPRNLAKSVTVE"
     gene            14153..>14533
                     /locus_tag="P5123_04865"
     CDS             14153..>14533
                     /locus_tag="P5123_04865"
                     /inference="COORDINATES: protein motif:HMM:NF013098.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HRX15622.1"
                     /translation="MIIIERLQEISFAVSNLDKSVSFYKDIFGFDIVEWQGGSKEVRM
                     QMGDINLRLCEVDNYSVSEESENYLTFFVDDYDFDDAVEEIEENEITTVFGPENIRNG
                     PENTKNGRKVVILDPDGNRLGLTTI"
BASE COUNT         4697 a         2219 c         2859 g         4758 t
ORIGIN      
        1 acattttgga ataaacataa tcaacaaaga ttataaggtt ttcgagattg aacatggcgg
       61 aagacatgat cttgaaaaat ataatgtcga gggcgactgg tctggcgctg cttttataat
      121 ggtcgctgcg gcattggctg gaactgttac tataaataat ctttcatcac aatcaagtca
      181 aggtgataaa gagattttaa caatcataaa aaacagtggt gcgcaaatta gtgaaaatag
      241 agattccata accgttcacc gttcgatttt aaaaccattt aattgtgatg ctactgattg
      301 tccggatctt tttcctcctt tgtgtgcttt ggctgctggt tgtaacggta aatcaagaat
      361 aagcggtatt caccgtcttg cctataagga gagtgacaga gccgcggttt tgattgagca
      421 gtttaaaaaa ataggtgttg gtattcaaat agaaggtaac gatatgataa taaacggagg
      481 gcaagttgcc ggaggggttg ctgagtcgtg taacgatcat cgcatagcaa tggctcttgc
      541 ggttgctggg ctaatttccg ataaaggtgt gacaatagaa aacgcagaag ctgttcaaaa
      601 aagctatcct gttttttttg aagatttgca tgatcttgga gcggaggtgc aatgaacagt
      661 tttggtaaca ttttcagagt atcacttttt ggggaatctc atggatcagc cgttggtgct
      721 gttcttgata atgtcccacc ggggatacct ctatcttctg aagactttgt tctggatatg
      781 aatcgtagaa aaagcggtaa aaaaggtaca actcctcgtt tggaatcaga tataccggat
      841 attgtttcag gtgtacatga ggggcttact accggtgcgc caataatgat catgataaaa
      901 aatgaaaaca caaggtcaaa agactattcg ctttttcgtg aaatgccgcg tcctgggcat
      961 tcagatttta ccgcacaaaa aaaatatggc ggttttaacg atttgcgggg tggtggaatg
     1021 ttttccgcaa ggttaacgac tgctattgtt gcagctggcg ttgtagcaaa aaaaatatgt
     1081 tctcctgtta actataaagc tgagattgtt catattggtg gaaacattga tatagaagca
     1141 gagcttgatt cggttatgaa agataaggat tctattggag gtctggtaaa gtgtacagga
     1201 ataaatatgc caataggtct tggagaacct ttttttggaa aatttgaagc acagttggca
     1261 catattatgt tttctttacc ggcagttaag gctttcgaaa ttggaagtgg tgtaaaagct
     1321 gcacttatgc gtggaagtga acataatgac tgtttttcag atgaaaacgg taaaacatta
     1381 acaaataata gcggtggtac tttaggaggt attactaacg gcaactccat agaatttagt
     1441 gtgacattta aacctacggc gagtatttca atacctcaaa aaactttcaa ttttgaaaaa
     1501 ggcaagattg acactctaat aatagaagga agacatgatg catgtttaac tcttagaaca
     1561 cctgtaatag ttgaagcctg tgctgcaatt gttactgcag atttgttact ttataatcgt
     1621 atatatggat aatgggagaa taaaatgata ctgaaggata ttagaaaaaa aattgattta
     1681 aaagacgcac aaatattgaa gttgctccat gaaagaatgg agcttgctgt tatgtcaaag
     1741 aagtttaaaa ataaaataga agattcagaa agagaagagg aactcttcaa aatgattgat
     1801 gcacattccg gtgtactgat caatcctgaa tttagcaaaa agctatataa agatattatt
     1861 agtgaaagta aacgacttca gggaaaaaat tataaattaa taggtttcca gggagaacag
     1921 ggagcatatc aggaactcgc ttcccttgtt tgggataaaa atttgatacc tactgcatgc
     1981 tcaagttttg ctgaggtatt taacggagta gagactggtc tctatgatta tggtatcgtt
     2041 ccgatcgaga atactcttgg tggtgttgta aaccagacaa acgatttaat gattcactcc
     2101 gatctttttg tagtaggtgc tgtagaactt gaaattcatc attgtctact ggctcttcct
     2161 ggaacagatc atcgtgatat cagaatggtt tattctcatc aacaggcact agctcaatgt
     2221 cgtaattttt tggctcgtca taatttagag ggcatacctt attataatac agcaggtgcc
     2281 gctaaaatgc ttatgcatga aaaaccaaaa gcatcagcgg ttatcgctag tcgtcatgcg
     2341 gcggatatct ataatttaga gattataaaa gaaaacatag aggatcttga tacaaaccgt
     2401 acaagatttc ttattatttc aaagaaggag agtagtgaaa aaggagagaa gtgctcagtt
     2461 ctttttagta ctgctcataa ggcaggaaca ctttttcatg taatagagat ttttgcaaaa
     2521 gagaatataa atcttacccg tatagagtct attccaaacc gacctggaac ttatgtgttt
     2581 tttctggact tcgaaggtga caaaaatgat ccacgagttc agaatgtgct taaaggtgta
     2641 agtgaagtta cgggtgaatt cagatttatg ggatgctatc gagaggtaca tgcttaaata
     2701 gggagcgtta aaatgagttc cgaaaaagta agcaaaaagc tgtcaaaaaa gagaataaca
     2761 aagaaaacac cggaaaaaag aggcaaggct caaaagcctg tgcctgtttc cggggggaaa
     2821 aatgcggttt tgattcttat tgttatggct ttggtaactg tgctcgtgtt tatgggggta
     2881 cagatgctta agttaaaaaa tgctccccat actagagttg aaaagtcgat cgtagaacct
     2941 gataagtctt tgactgatag taaaaaaagt aaagacgaag gtaagaaaaa agaaaaaccg
     3001 gatgacggat ctactgttgc cggtgaaacg agtactgata aagccaagaa accagatctt
     3061 atcacgggaa atatatatct tctattcttt gatgaacgaa gtgagttaat tacattacgt
     3121 ccggttgcaa ggcgttttcg tagtgaccaa ttattaacgg atgcctttag catgttagtt
     3181 aaaggacctc tcaaagaaga aaaaaataaa ggtttcgaaa caagtctttc accatccatg
     3241 cgtttcaata cgattaaggt aaaaaataaa attgcgtata ttgatataaa tcccgacttt
     3301 gtgaaggatt cttttggcga tattggtgtg gcacgtgtta atcaggtttt tttgacatta
     3361 acacagtttg acagtattaa tggtatagtt atcaccgtta acggcaagaa aattgacaca
     3421 atggatgacg gtcgacattt tgtttggcct atgacaagga gattataatg tacgagatag
     3481 aaatcaaggc ttattgtaat gatctttcct tgattgaaaa tgaattggaa ttggaaaagg
     3541 cgcttttcgt tggcgagtat aaacagcatg atatctatta taaccatcca ttacgtgatt
     3601 ttaaagaaac tgatgaggca tttagaatac gttctgtcga tggtaatcaa tttgttacct
     3661 acaaaggccc gaaaatttct caaacagcta aaacaaggat tgaacttgag acatccatag
     3721 gtgaattcga atctttccga tcaatacttt tatcacttgg tttcaaagag ggtggcgaag
     3781 ttataaaaat tcgtaaagag tatatactaa acaatgttac catctgtctt gataatgttt
     3841 atggagcggg aagttttgta gaatttgaaa ttatgggatc cgacatcgcc aaaggtgaac
     3901 aggcgctatt tgaaatggcc gagcatcttg ggcttagacg tttcgaaaga aaatcatatc
     3961 ttgagcttgt tttaggataa gtattttgtt ggcgctattg gggggattta tggaacaaaa
     4021 agtatatatt tatatttgtg atcattgtaa aaaaatgtca cgggttaata aagatgatcc
     4081 tataccccac tgctgcggcc agttgatgcg tcgttatgat gatgcaaata acaaataaac
     4141 ttcatatata gaatcttaat aatttttaac atccatccaa aatttttatc attattgaac
     4201 atgtcgtttg ttaaataata agcggttatc atgttgtgag agataagtga taatattcta
     4261 gatcaaaagc ataaaaataa tgcaatagaa tcggttgcat tgatgtatca aataaaataa
     4321 taaaaatata tttagacttt ttaaaatttt tattattgtt attgccgata tttgtataag
     4381 tatattaacg tccaggttta ttcatggtaa aattaaactt taacatgttt caagagaatt
     4441 gcgttaatat gtattttata ataagctttc ttataacaaa ttcaaattta tactaaaagt
     4501 agatgaaaaa aaatataata aataacaaaa aagcgatttt aaaattagta atagttatta
     4561 ttgctattac gcatatttat agctgccagg atggacacga ttatattttt tcagagagtg
     4621 atataaggaa agaatcaaat gaaactaaga catttgataa taaatctgaa aatatgtcgg
     4681 atgatataga gactgatgac gagtttaata atagtataag tagtactctt gcaacaaatt
     4741 ttacaaatac tatgtacggt gttggatcca tattgggtag tggtactgtt tggtattatt
     4801 tggaaaaatc aaacaagcga gaccagtttt ttaattccgg agatgatatc cgaactcgtt
     4861 ttacatcatt tgatgcctgg aggtttgacg ataatctttt tgaaactaac gctgtcagcc
     4921 accctatggc aggtgctgga tattttttat cggcaaggtt aaacaactat aaccagtatg
     4981 aatctttttt gttttcgtta ggaggatctc ttgtatggga gtacttctgc gagtttcagg
     5041 aagtagtcag cattaacgat atgattttta ctcctgttgg aggttacgca ataggtgaat
     5101 cattcttcca actcgtaaat ttttatgctg ataattcatc aaacagttca ttgggtgata
     5161 ctattcaata ttatacaaaa tctcaggatg gacgttataa aaatggactg cgtaatagtc
     5221 aaggaaacaa tcctttttgg tataatgctt atctttacgg tgggtattct caaactgata
     5281 gtgaacatta tacaacacat tttggtcttt atacacaatt ttatgggaca agaggagtaa
     5341 aagagaatgg ctattataat aaatttcttt ataattcacc ctatacaaaa attgagattg
     5401 attttggtgc aggcaatacc tggttaaaag actgttatct tttcttcgaa tctggacatt
     5461 tatcttatgt taatcaaaac ctacataacg aaaaggccgg ttatgctttt attgccactt
     5521 tgacgactgc attcgaatat agaaattact attatcaggg ctttcgcgat cgtattggtg
     5581 cggtgagtcc tgtgaaactt aatactgatt ttctttttga ttacaaagat ttttctatta
     5641 atttggaact tggtttatca cccaatttcg cacaggtgag ccctttcggt ttgggactat
     5701 tttatcaaga cgggtaccat agtgactatt taagagaaca tggcgcgcat acaacggcaa
     5761 atcgtggtta ttattttggt tatggtttca gttcaaagaa ttcactaact atttcgtaca
     5821 agaaatatca aagtggactt agttacaact ttgattacta caaatcaatt aatactgatg
     5881 gattaacaaa tagagaagaa ccgaccaata agaatttcaa aatgaaagat gagagatctg
     5941 tattttcatc atggttaaaa tatgatatta ctgacgcttg gagtctggtt gcaaaatatt
     6001 atcgcaatga aatagaagga acagtggaag attacagtag atccgatttt gagagatcaa
     6061 ttaaactgct gacattatat aattttagat aaaattattg gtatttttta tctatggtcg
     6121 gtggatttct tttgtagaaa accttcttag ataattcgta agtaaaagat gaagagtaaa
     6181 ataatttttt ttcatgactt cttgccgcac attgtagtga agaacttcca ttctgaaata
     6241 tcaaaaacca tgattcaatt ggtgaaaatc gtaccttgtg tatagatgat acatctagga
     6301 aaagggtttt gcaattatcg ggtgtattat attttattgg tatcatactc ggaaggtgat
     6361 cgtgagcaga aatccagaca tctacggtat aatcttttat aacatcccaa aatctgtctg
     6421 agtcttttat tatcatagtc cagtttaagg tcgaaacaag ctggctctga aacaggggag
     6481 catgagttac tgtaattatt atagaatcct ggttattgat tacaatattt ttccaccatt
     6541 cgaatacatc atctgatatt atggtatttg ctttttttgt ttcatcggat aaaaaaataa
     6601 aaagaaggtt cccacatcta actgtgtaat ggaggggctt acctgattgt cgtttatatg
     6661 tctcaatatc atggttgtca tgatttccgg caatttcgaa ccagtaggta attcccgttt
     6721 tttttcgaag atcctttatc caattccaat acatgtcaga tgattgccta tgatttatat
     6781 ctccggctac aatggccata tcgggaacaa agggtaaaga tctgacatca tcaatcgcgt
     6841 attcgtaatg gtatttttcc cagagataac gaggttgtat gtctgaatga gcccaaatga
     6901 ttaatgtttc agatggttca acattgtcat taataataag ctcagttgaa caagaaaaaa
     6961 aagttgttaa taataaaaga taaaaaaaat ttgttttcat tatgagccct gtgcgttagg
     7021 tttaaattta ctaccaatac tgaaagtatt ggtagtaaat gaaattccta agcaaattat
     7081 tcgacaatta tgaattttcc aaaaatccat cctgtagtat tagaagtttt aacctgatac
     7141 catgtctcac cagcaatttt tttgactgaa tttttatcca cagtaccgga aataatttta
     7201 ccatctgaaa ttgggtaact gactgtataa ctagattcac tttcatctgt gattgttatt
     7261 cctttaccgg catccagttt gattttttta ccagactcca tagttatttg agtctgacca
     7321 acaagaacgg cgccgtttct attggcacta ttggttttgg caataacatt aaccttgcta
     7381 ttattatgca gctgtctaat tacatcagaa gagacgtcag gttgcactcg taaattaaca
     7441 ttgttaccgg taattgtgcc tttagcatta atttcaatta ttgagtcgtc aatttgagct
     7501 ttaccaggtt cggtcgactc aataggggct tcgatggaag gagtaccttt gttgtaaaaa
     7561 tatatggcaa tcataccggt taacaagatt acaattatta tagctacagg aatatagggt
     7621 tttgatttaa agatctgtaa ggggttaaac acaaacttct gactgatttt tgtatctgtt
     7681 ccttttttct ccttttttgt tgggggtgtt tttttgttgg atattgaaaa agtgtttttc
     7741 ggttttgaag cgggtttgtc atttaaagct tgaattattg ctctttgctg tttttttcgt
     7801 aattcaagct ccttttgtaa ctcctgaagc tttttttgac tatccttgat tttaaccgat
     7861 gctgcctttt ttgaatcatg attaccattg ttggcatccg cggtaagctg ggaaattact
     7921 tttacaagtg gttttaaatc gcgttgaatt tcattaattc gtttttcagt acctgataat
     7981 acttgcatgg ctttcttttt taaaatctga agctcttttt tctgttccga taaagtttta
     8041 gaatcatttt gattatttga tttttcagct gccgaaattt gctttctgat ctcggttatt
     8101 tgttttaaat cggcggaggt taatttgctc atatatttct ctcttgtaaa ttttggataa
     8161 ataatcatta agttatgacg atttattttg atattatctt tattgtagtc aagctgtttt
     8221 cattttgatt atttaatgtg gttactcatt tcggtttttt atagcttaat aaaagcaaaa
     8281 ttttgtgata aattttagtg atatttcgaa tggtaacggt taaattgatt cttttgttca
     8341 tcgattttag aattaattac gagttattaa tattggtaac atttaattat ttcatttctt
     8401 tgtggcttta ttgtacttgt caaaatgtat ctttgttgca ataaatgctg ttgctggaat
     8461 agcggtaaac atccccaagc tgcctacaaa tgattttata atttcgatta aaacagggtt
     8521 atgtgaaaaa atcatcgaga ttggcatttg tgcgtcatac ttcatataaa taatcagaac
     8581 tatcgaaagg gagcttccca cataggctag aataagtgtg tttaccattg aaccaagtat
     8641 atcttttcca atagttaggg atgagacaaa ggccttttta aattcaattt tcggattggc
     8701 ctcaaaaatc tcacttgtcg cggatgcaat agaaacagct acatccatta ttgcacctaa
     8761 tgctgcaatg atcatacccg aaagagcaag attgcgaaga tcaattcgat tctcagcaag
     8821 ataaaaaact tgtatcatat cgtcggtaat tatgcccgca aggtgcattg accacccaca
     8881 aattagtgca agaattgtag ccacgataac tcctgatgtc gcacctgtta ttgcagccgt
     8941 agtttttctt gtgattcctg taattaccgg tattgtgata aaaatggata tgattgagat
     9001 aagaacagtt aatacaagag aaggataacc ctttacagtc aaggggacga aaacaaaaaa
     9061 tatcaaccca acagtaatca ttagcgccaa caatgaaaaa agcccctttg ttttcccaat
     9121 accaacaatc gttatcacta atagaatcat cactattgtt atccctacag tattgtccag
     9181 atcataaagc gctatataat cgtcagtttc gtcttccatc ataatactgt taaaaccgat
     9241 aaatatttta tcgccttctt tatatctgct gtaggcaggc atactgtctt ggcctgcgta
     9301 cacgacctgt tttgtctctc ccttaaaatc accggacaga atttctagag aaaatatggt
     9361 ttcgatttgt acttcaccgg tggtttcatc ggttaatcgc cttgtttcaa ttgtatttat
     9421 tttggcttta gcgtaggtaa tttcctgatc aaaatcctga gctgaaatgt ttgtagcaaa
     9481 acagattaag attattgata aaaagagaaa aattccagat cggttcatat atcctccaat
     9541 aatgttctta aataaacgta aaatctataa ttataccgat attatgcacg ttaaaaagtg
     9601 taaagaaaag attaccttgt aagggtacaa tttgtaattt ttttatcttc tataatggat
     9661 aaaatggttt aatcaagcga atttatgttg tataaacaga caaatagttg acgtagagaa
     9721 ataatcagga tattatcaac ttatctattg tgtcgtataa tgacgcgcaa aagttttaag
     9781 taatattttt tgtctgtata ttattaaagc tgttatgact gtcggttatt gttttttctt
     9841 ataaaattaa ataaaattta cggtatttat ggcagaaaaa aatattttat caatactaat
     9901 tgcagcttac ctgcctgatc ataatgaaac gcttcttttc gcagggtttt ttgatacaat
     9961 acgcattcaa ttgctgctct attttaatac agtgattgta tcttcagaga ttcaatcaac
    10021 tgttaaagat ttggatatcg atgagtttgt tcattttata tttgaggcca aagataacta
    10081 tacaattgat accgatctta ataatcgcgc tacggattat ttcgttgggg aatttaacgc
    10141 aaaaacgata gaaagtatta ttacgaaaca ttcacaaaat gacacaggag cactaagttt
    10201 taactttaaa agaatgatag atcatctgct tcaacttgat acaacacaaa tacaaatgaa
    10261 gatttgtatg acaagccatg gagcattgga aatatttaat gcatacgaac gacgtattag
    10321 tcacaccgtt tccgaaaata acgaaattgc gtcaaaatac gttggttcaa acagggattt
    10381 aatcaaaaaa atagtggaat cttcgattgc cgattccact gttgctgttt ttacggttag
    10441 taaagaggga aaaatagtat cctgtggttc ggttatttat aacgagttat ctaacaagtt
    10501 ggttcataaa aactatatat atgcgcaagg tatatcattt gatgaatgta atcttgctaa
    10561 taaccttgtt gatgtctttc attatattca agaatttagc gaaaattgca caaataatga
    10621 tgaggtcacg aataaaattg ataatgctgt ctccaaaata aatattgatc gtttggcagt
    10681 aataatgaag tcagctgatt ctgacaaggt gaactccttt ttttccagaa taatgcctcc
    10741 cgaaattaca agcggaaatc tggaaatacg tgttcaaaaa tttaattcaa ttattcttga
    10801 tatagctttt gaaccggtac tgttaggtca aaaaagtaag gagaaagctc ttaaagaaga
    10861 gacagttcca aaatccaaag ctcaagttga agttgatctt gtaatttctc cgactaaagg
    10921 agtcaaggtt tcagagttaa aatcaggttc tcgaatctat gtaataataa atggcacaac
    10981 aattataggt cgaaaggtca ttaatcaact taatctgctt gatgagaaca gagtgaaacc
    11041 tgtcagtgct gtagtagaag aaattactaa aacttctgat ggttatatag taataacaag
    11101 aattgcctca aatctccttg gtcgcgcgat tgaagaggaa gatgtaaaag taaagtctgg
    11161 tgatcctgtg gttgataaga aaatcgaaaa atcacggaaa agtcttcttg ttggagtctc
    11221 tgtcggtctt ggttttattc ttttggcggc aatatcgtta tgggttcttc tgtaaaagag
    11281 gcctataata atgattacat ccattatctg tagcaatatt tttgaacgtt ttttcgagtg
    11341 acaacgagaa aaattatctt taaaagcact tgaaaaatag tatctttcga aaaggtaatt
    11401 tttcaaagat ccctattatc aaagctattt tttgttgtgg atttttgaaa cggtggaaat
    11461 aactatattg aaattgttat acagaataaa aattttcact cttcttctta tgatttttac
    11521 tatgccatat aataaagtgg caggtgaaga ttcttctgcg catgattccg gacccactga
    11581 aaaatattat agattgttgc atttatatta tctgtggacg accaacttct atcgtggtga
    11641 taactattac cacagagaaa tcggatcgac tctgtattct tatatcaaca gtaattttga
    11701 aactccacga attttgggac gcatagagca gcttcatggt caaggtggtg ccgcttacag
    11761 ttgtttccga ttcagttctt ggttaacaat caagttcgat ctacgcgaaa aagctgttgt
    11821 cgaacttcta tcttcattaa cccaaagaga gagggctctg ggtatagata ttggtatctc
    11881 tggtactatc tatcgttaca aggttattaa tcgtgaaaaa gatcgttcca ttgaaattta
    11941 tttacgtgat gtaaatatta ttaaagataa gaccaaagcc ccacctgatg agagttgagt
    12001 aagatatttt ttattttttt atgctcataa aatacttgaa acttgttata tccgctaatt
    12061 aatggaaaat ctattgtttg gggcttaatc acgggtaaat tccgtttttt gctctcttcc
    12121 caaatacacg ctttttttgt gaatcattta aaaataaggg gaaatactat gtgcggcatt
    12181 gtcggatata taggtaccaa agctgcaact tcggttttgc ttaaagggtt aaagtgtctt
    12241 gaataccgtg gttacgactc atcaggtata gctttgttaa acgattcact ttttgtttgt
    12301 aaaaagaaag gtaaaattat tgaacttgaa aaagagttaa atggtttaag taaagattta
    12361 tcaacatata ctgtgggaat agcacataca agatgggcga cccatggtga accttcagat
    12421 ttaaacgcac atcctcatgt cagctcatca ggtaagatcg ctgtagtcca taatggaatt
    12481 atcgaaaatt ttcagatttt acgtgaaaaa ttagccctta aagggtttaa aatcacaacc
    12541 cagaccgatt ctgaaattat tgctcatctt atagagtact atgttgacaa gtataatgac
    12601 tttttaaccg gagtatttgt tgcacttgaa aaactggaag gtacatacgg tattgttgtc
    12661 atttcgagag attatcccga tagaataatt gcagcacgta atggaagccc gttaatactt
    12721 ggtgttggag agggcgaaat aataatcgca tctgacgcta gcgccatagt cgagtatact
    12781 cgtaaagtca tatatatcga tgacagggaa gttgttgata tacaaagaga ttcttaccag
    12841 acctatgacc ttcttcaaaa gcaggtcgaa aagccggtag aagatataga ttgggatata
    12901 gagacaatcg gcaaaaaagg atatgatcac ttcatgctca aagaaatatt tgaacaggtc
    12961 gagagtgtta aaaatacttg ccggggaaga ttgttaacag aaaccggaac cagcaggctt
    13021 gacggactga atcttaaacc agaagatttt tccgagatta aaagaatcat tttgatagcc
    13081 tgtggaacat catggcactc tgctttgatt ggagaatatc ttattgaaga atacgcgcga
    13141 atacctgtgg atgtcgagta tgcttccgag tttcgttata aaaaccccat tttgacaaaa
    13201 tcggatctgg ttatagttat tagtcaatcc ggtgaaacag cggatactct tgctgcgttg
    13261 cgtgaggcaa agcagaaggg tgtaaaggtg ctgggggtaa caaatatagt cggaagtact
    13321 attgctcgtg agagtgacgg tggtgtttat ctccatgccg gtccggaaat aggtgtagca
    13381 tctaccaaag ctttctcatc gcaggtcatg gttatgataa tgattgcaat tcattttgct
    13441 cgtcgcaaag atatgagcct tgaatctgga caagttctta tcgaaaaaat agaaaagctg
    13501 cctcaattaa tacaatcaat gcttgacact gccgaggata taaaaaatat agctgaaata
    13561 tacaagaatg cttcaaattt cctttattta gggcggggcg tacagttccc tgtcgcattg
    13621 gaaggggctt taaagctgaa agagatctcg tatattcatg cggaaggtta tccggctgct
    13681 gaaatgaagc atggacctat cgcactcatt gatgaaaacc tgccagttgt ttttatcgct
    13741 ttgaaagatt caatatacgg aaaaattgta tcaaatatgc aagaggttcg cgcccgtggg
    13801 ggcaagatca ttgccattgc tactgaaggt gataatgaga taagtaacta tgcggatcac
    13861 gtaatttttg ttcccgatgt agaccggatg ctgtcaccga ttttaacagt tgtaccgtta
    13921 cagcttctct catactatat cgctgttatg agaggatgta acgtggatca accgcgtaat
    13981 cttgcaaaga gtgtcactgt tgagtaaaaa tatcaattta ttgacaatat tgagctttgt
    14041 ggtattatat tgatagtttt attagtagca aattatgcga atcttactgt ttccaataat
    14101 gcgcgtttgt atttttaaaa gtcaattatt tagaattacc aagaggttat ttatgattat
    14161 tattgaaaga ttgcaagaaa tcagttttgc tgtttcaaac ctggataaat ccgtctcctt
    14221 ttataaggat atattcggtt ttgatatcgt agagtggcag ggtggctcca aagaagtgcg
    14281 gatgcagatg ggggatatta accttagact ctgcgaagtt gataattatt cagtttctga
    14341 agaatccgaa aactatttaa cattttttgt ggatgattat gactttgatg atgcggttga
    14401 agaaattgaa gagaatgaga taactactgt ttttggtcct gaaaatattc gtaatggtcc
    14461 ggaaaacaca aaaaatggtc gaaaagttgt aattttggat ccagatggca atcgtctggg
    14521 gctaaccact att
//
LOCUS       DAOTGZ010000048        14434 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_3064
            NODE_5032_length_14434_cov_4.718409, whole genome shotgun sequence.
ACCESSION   DAOTGZ010000048 DAOTGZ010000000
VERSION     DAOTGZ010000048.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868088
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 14434)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 14434)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:22:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,785
            CDSs (total)                      :: 2,751
            Genes (coding)                    :: 2,732
            CDSs (with protein)               :: 2,732
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 12 of 19
            Pseudo Genes (internal stop)      :: 5 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14434
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5032_length_14434_cov_4.718409"
                     /isolate="AMR_MDS_3064"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..737
                     /locus_tag="P5123_04870"
     CDS             <1..737
                     /locus_tag="P5123_04870"
                     /inference="COORDINATES: protein motif:HMM:NF023739.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="exonuclease SbcCD subunit D C-terminal
                     domain-containing protein"
                     /protein_id="HRX15623.1"
                     /translation="DYYSALHDAAFKKKNELQSSAPVIVMGHLFAAGGKTVEGDGVRD
                     LYVGTLEHVPASVFKSDIDYLALGHLHSPQKVGGKDYMRYCGSPLPMSFGETGIQKKV
                     IEVCWDGNSRTVKEIDVPCFQRLERIEGDIDTIKKVIKQFKNQKQSIWLEIEYTGDDV
                     ISSLSEQIIEMTKDSLLEVLLLKNRKRYDAVLKQSVYTESLDDFNELQVFTKFLDINR
                     IEDGQRPDLIKTYKKVLQLIHEDDKD"
     gene            740..1876
                     /locus_tag="P5123_04875"
     CDS             740..1876
                     /locus_tag="P5123_04875"
                     /inference="COORDINATES: protein motif:HMM:NF024867.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HRX15624.1"
                     /translation="MRILKLRFENLNSLYGEWEIDFTSSMYMSDGIFAITGPTGGGKS
                     TILDAISLALYGRTPRLNNISKNSNEIMSRHASQCSSSVVFETQKGIYCAVWRQHKAN
                     KKADGNLVDSTHEISDFTTSKIIETKKRQVVEEIERLTGLDYRRFTRSMLLAQGDFAA
                     FLLTDAGERSMILEQITGTGIYTEISKMVHLVTKDKKSALAEIQNLLDDVNVMSLEEE
                     NQADAKLKGLAELASKTVEKLEFINNAISWRRNLADFEQRIRETEKKEQFAKEEQKLF
                     EKEKERLVKGQRAAELEADYKELCSVRLELQNKKNSLENISNEEIPLLKSIDDSKKKA
                     NDLSQKLKNIRNENAILLKTVQEVRILDNRIDEKRKEYKNMIVS"
     gene            2008..3987
                     /locus_tag="P5123_04880"
     CDS             2008..3987
                     /locus_tag="P5123_04880"
                     /inference="COORDINATES: protein motif:HMM:NF024947.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SbcC/MukB-like Walker B domain-containing
                     protein"
                     /protein_id="HRX15625.1"
                     /translation="MRECDLLCDKISQITDLEREIREQEEERESANSIHEKQKQTLNL
                     LLQKQSKIIRDSEAYKTDIKKIAADMDSLLGIRQIDDYRTELEKLKEEKYRRSAVSDY
                     QKERSNLTKGEPCPLCGSLDHPYVSKKLPDVSETDIRLADVQKIIGQYDLLEKQKKVY
                     IDNLNEKEKDLHGIESEIKVFNANSEAYLKSVKKIEADTEKHKIKKQNLLKNLEPILV
                     NLGIKIVDLKNPDKIINSLCERRDGYKFYCDAFELCEKELNILKIQYGELDAGIKAIT
                     EGVNRHKEKTVILGSDGMALSKERMRIFGEKDCDKAESEASAAIEECEILLEKEKDNL
                     NTLQLRLQKIKTSIETLNKDIAQFAERLANLLQGFKCALDKADFSSEDDFVRCRMSAE
                     ERREVEKRGMDIQKQLVQVLAYLKELKDEYNKEMSKKLSTSDLNKLLADKEELSKELN
                     EVHEKKGSLQQRIEDNNKAKKERKSRENRQIKAKKEYELWSNLDSLIGSADGKRYRTF
                     AQGLTFGLMVYNANIQLRKMSERYLLVQGSDNPLELNVIDNHQGGIERTTKNLSGGES
                     FIVSLALALGLSRMAGKNVRIDSLFLDEGFGTLDEDTLDVALDTLSSLHSEGKLIGII
                     SHVESLKSRISARIEVKPETGGRSLLEGPGCKRIG"
     gene            complement(4038..4110)
                     /locus_tag="P5123_04885"
     tRNA            complement(4038..4110)
                     /locus_tag="P5123_04885"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(4075..4077),aa:Val,seq:tac)
     gene            complement(4101..4373)
                     /locus_tag="P5123_04890"
     CDS             complement(4101..4373)
                     /locus_tag="P5123_04890"
                     /inference="COORDINATES: protein motif:HMM:NF012442.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0030261 - chromosome condensation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HU family DNA-binding protein"
                     /protein_id="HRX15626.1"
                     /translation="MTKKDMVNIVHKDINLNKQDINKVVNLFLKAIVDEVSKGERIDL
                     RGFGTFYLQKKKGRSVFSPITQKNIDVPPRFNVVFKSSKTNQSTGD"
     gene            complement(4382..4657)
                     /gene="rpsT"
                     /locus_tag="P5123_04895"
     CDS             complement(4382..4657)
                     /gene="rpsT"
                     /locus_tag="P5123_04895"
                     /inference="COORDINATES: protein motif:HMM:TIGR00029.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000312 - plastid small ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S20"
                     /protein_id="HRX15627.1"
                     /translation="MANNNSTKKRIRQAAKRNMRNSQNKSALRTASKKLLSLLDAKAE
                     IKSDEVVALQNNFAKTVDKVAKNNVIHWKKAARLKSRMAKKVNSALA"
     gene            complement(4808..5353)
                     /locus_tag="P5123_04900"
     CDS             complement(4808..5353)
                     /locus_tag="P5123_04900"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRX15628.1"
                     /translation="MKKTIVIIALICNFTLLLGENLYKATRSICDWSLTQSQITGKTA
                     DFNAQIHKVPDKELSTYKNYILNHISGLDFDGSLKPEIYYQEKDQIYRDFIFLNNRLS
                     AISCRYTLLDEKQFMTIFSDLKKDFGAPETKREQTTTTHSFKKGDTTALFMIRSKAGG
                     FEARLYFYTNDLFKRIIMQVE"
     gene            5608..5808
                     /locus_tag="P5123_04905"
     CDS             5608..5808
                     /locus_tag="P5123_04905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004513274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Trm112 family protein"
                     /protein_id="HRX15629.1"
                     /translation="MIDSKLLNILACPACKGDVEYDAKAQSIRCLECALVYPVVDGIP
                     VMLIDKAQKPEEDEGKKEKDAE"
     gene            6034..7185
                     /locus_tag="P5123_04910"
     CDS             6034..7185
                     /locus_tag="P5123_04910"
                     /inference="COORDINATES: protein motif:HMM:NF025010.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SurA N-terminal domain-containing protein"
                     /protein_id="HRX15630.1"
                     /translation="MVKSKNIVVKVFSYILVGILTILLVISLGSPGLMDKAMLNQNTL
                     AAVNGKKIDVQDFGYFLNTDQRFYNRASEIRDDEQLLNYARSVYLQRVLTIQYAQEIG
                     IVVVDDQVVDYISSLGNFKDEDGKFMPDRLDQFLKYYNMSYNQFFKRTYDVLVLRELD
                     NLIVNGTGVSPDDIHMDKTVNNSKITLRYGFLSNAEIKKRYPERLTISDKEIDDEVER
                     LKETITEPIDDTELLKKQAKNELEVRKLSELKLEISSKINSLALEGKPFTEGLAVLDV
                     KAEDSKPFKIGSPVYSAEESKGLLLDLYTNSIFYEDCLKVKQGELARAVDVSSGLYVF
                     TPIEKIVPFEKDGELTAYDSYMAHQERSLFVENALLSPFIEKSRVIRNY"
     gene            7324..7788
                     /locus_tag="P5123_04915"
     CDS             7324..7788
                     /locus_tag="P5123_04915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020774267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis protein CheX"
                     /protein_id="HRX15631.1"
                     /translation="MNINAEYVNPFLEAASAVFKSVMNVDLRRGKMIIKENPEPSHDV
                     AIIIGITGAINGEVVYSMGYNMVTKIAEILAPGMTEEQIKSEYKDIVGELANMITGNA
                     MNLFAYSGKSIDITTPTVVNGKDFTITLVQQTTLGINLYSPMGQLEMNVALK"
     gene            complement(7806..8189)
                     /locus_tag="P5123_04920"
     CDS             complement(7806..8189)
                     /locus_tag="P5123_04920"
                     /inference="COORDINATES: protein motif:HMM:NF015639.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YkgJ family cysteine cluster protein"
                     /protein_id="HRX15632.1"
                     /translation="MEKSPPCDKCKAQCCRHLAVEIDKPTTKKEYDYIRWYLLHKNVS
                     VFIDHNNDWFIKFNTDCEMIQKDNRCGCYESRPKICRDYPSKDECCEFEGEDDYFTHM
                     FDTEAEFSDYMDSKKRDWKYKKTNS"
     gene            8537..8896
                     /locus_tag="P5123_04925"
     CDS             8537..8896
                     /locus_tag="P5123_04925"
                     /inference="COORDINATES: protein motif:HMM:NF016056.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF393 domain-containing protein"
                     /protein_id="HRX15633.1"
                     /translation="MITESWTLLYDRDCPLCARFAAIVQNLDHENMIDFVPLQDYCKK
                     EIRFSCEELLKTVHIISEQGQVLKGGDAVCKVLMLVPASRPYRWLLQSKVGRRVSNTV
                     YFALNSFRRCYKCNRRR"
     gene            8899..10203
                     /locus_tag="P5123_04930"
     CDS             8899..10203
                     /locus_tag="P5123_04930"
                     /inference="COORDINATES: protein motif:HMM:NF012772.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HRX15634.1"
                     /translation="MNIIMVYSDELVAQSVAAVSAVHAISMVKANESEGIDFTNYSLV
                     LLNVDDILCDRLLDKINKKVPVLRLNNSNQFIKMMNYVTKGLVGTVSLPLSGAFLYEI
                     IDSLSRQVVTLPENSNDKYQFYYNGDKIEVCVNPDFLLRSVRIAEQDIEQKKIAVTHI
                     TSNKKVDAASVNDSLSRLQEEVVEGLVNDEFSLAYQPVHYLNDQMLYGFEALIRWNRK
                     GLLISPDDFIPLVEKTDVVYALNDWVLNQVLLDLEDWNVKFTTNGDNLRVNINLSAHL
                     FESPGIADKIIESVKTAKIDPRQLGLELTESAFMDSKEIANLALLKLKSNNFPIYMDD
                     FGTGYSSLSYLQHFPVDILKIDKSFVKWITFDEDSEHIVSSIIGLAHGLKMKVVGEGV
                     EERAQVDFLKERGCEFGQGFFFSRPLKKNDASLYLADYHNRR"
     gene            10211..10843
                     /locus_tag="P5123_04935"
     CDS             10211..10843
                     /locus_tag="P5123_04935"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HRX15635.1"
                     /translation="MVRILVVDDHPIIYQGLVQMLQDDLRFKVADNAKTYDEAIIKLE
                     KHKPDLLIVDIGLRSEKNGIDLVRFVTENYPETKTMVLSMHDEEIYSVRSYNAGARGF
                     VTKSDFTERIVEAIGMVMKGELYYQNGAPLVEHKGDRLKQQFSRLTDREIEILRCIGM
                     GMKNSDIADKTGVKEKTIHSHRLRIRNKLQLESTADLIRLASSYIDDNVI"
     gene            complement(10935..12041)
                     /gene="ugpC"
                     /locus_tag="P5123_04940"
     CDS             complement(10935..12041)
                     /gene="ugpC"
                     /locus_tag="P5123_04940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018248258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sn-glycerol-3-phosphate ABC transporter
                     ATP-binding protein UgpC"
                     /protein_id="HRX15636.1"
                     /translation="MATVVLKDINKIYPNGFQAVYDANIEIQDKEFVILVGPSGCGKS
                     TTLRMLAGLEEITSGDLYIDDKRVNDIHPKDRDIAMVFQNYALYPHMTVYDNMAFGLK
                     MRKTPKDIIEKQVKEAAQILHIENLLDRKPKQLSGGQRQRVALGRAIVRHPKVFLFDE
                     PLSNLDAKLRVTMRAEISRLHTKLQSTMVYVTHDQTEAMTMGDRIVVMKDGFIQQIDT
                     PMELYNNPNNMFVAGFIGTPPMNFHPVSLTGDKLTEKGINLTPTPEQLAKLKEYGSKN
                     IVFGVRPEDFTFEENPTNSINAVIEVVEPLGSETILHVRLDSDSNMVLKADPTKQFSV
                     NQSVKLGVVISKCKFFDATDKVDPTGKSENPCIV"
     gene            12524..13495
                     /locus_tag="P5123_04945"
     CDS             12524..13495
                     /locus_tag="P5123_04945"
                     /inference="COORDINATES: protein motif:HMM:NF014647.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="BMP family ABC transporter substrate-binding
                     protein"
                     /protein_id="HRX15637.1"
                     /translation="MKNIKKISSLIIAMLIVLCGCSKNYDPINVGLVLTVEGPGDESF
                     NDNSLAALERAKLDYKINYDYKVPTSMADIDNTIEYFAKNNYDLVIVTGFLMHDACES
                     IADKYPETDFIIIDSLVKRPNVKPILFNEKEGAFVAGAVSALVSRSGNVGFIGGMDLP
                     FIKEILEGFRAGVQHQNTKIRVASVYLNGENPFNDPLQANKEAEKMINEGIDIIFHIA
                     GNSGIGIVKACSENRVFAIGEKGQYGSSDDTILANIVKNPENAVYRLVVERLKGEFDN
                     APVLSGFSENGISIDFYPYKSGNDVSNLQETVQAAQEALLSGKISIE"
     gene            13646..>14434
                     /locus_tag="P5123_04950"
     CDS             13646..>14434
                     /locus_tag="P5123_04950"
                     /inference="COORDINATES: protein motif:HMM:NF024583.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GAF domain-containing protein"
                     /protein_id="HRX15638.1"
                     /translation="MLSRYKSLICFLLLYLFGLLSGFFIKTGVSNYKQLSSLAFLIIP
                     LLIFIIFCIFVILISRSRAVKLYLRLIALIRSLPDIHIHSLSDAKNISNMFAKMGYFE
                     QIISVYQSKIRARYRHLDSNSMIQTVLLSLNNLHIEMDNRNEFFKAVLESAITVIPQA
                     DSGALLVLDCDGILHYEAVIGFDIANVSPVELKKTEIYKRNKTHIFEPCIIRSAFSDN
                     DRGETQDFHSAVCAPINIEGNLFGVLVINSIDENAFGEDDLVLIS"
BASE COUNT         4710 a         2230 c         2962 g         4532 t
ORIGIN      
        1 ctgattatta ctcggcgtta catgacgctg cttttaaaaa gaaaaatgaa ttacaaagca
       61 gtgcgcctgt aattgtaatg gggcatcttt ttgcggctgg cgggaaaacg gtagagggcg
      121 acggagttag agatttgtat gttggtacgt tggagcatgt accagcttcg gtatttaaat
      181 ctgatataga ttatttggca cttggccatt tgcattcacc gcaaaaagtt ggtggtaaag
      241 actatatgcg atattgcgga tctcctctgc caatgagttt tggtgaaacc gggattcaaa
      301 aaaaagttat tgaggtatgt tgggacggga acagccgtac agttaaggaa atagatgtac
      361 cttgctttca gcgtttggaa cgaatcgaag gtgatatcga tactatcaaa aaagtaatca
      421 aacaattcaa aaatcaaaag cagagcatat ggcttgaaat agaatataca ggtgacgatg
      481 taatatcttc attatctgag cagataattg aaatgacaaa agacagtctg cttgaagtgc
      541 ttcttttaaa aaacagaaaa cgttacgatg cagtactaaa acagagtgtc tatacagaat
      601 cacttgatga ttttaacgaa ctgcaggttt ttacaaagtt tctggatatt aaccgtattg
      661 aggatggaca aagacctgac cttataaaaa cctataaaaa agttttacag cttatacatg
      721 aggatgataa agactagtta tgagaatcct gaaacttaga tttgaaaatt taaattcttt
      781 atatggtgaa tgggagatag attttacatc atcaatgtat atgtccgatg gaatttttgc
      841 cattaccggg ccaacaggtg gcggaaagtc tacaatactc gatgcaattt ctcttgcctt
      901 gtatggccgg actccaaggt tgaataatat ctcgaaaaac agtaatgaga ttatgtcgag
      961 acacgcatcg caatgttcat cctctgttgt gtttgagact caaaaaggta tatattgtgc
     1021 cgtctggaga cagcacaagg ctaacaagaa ggccgatggc aatttggtcg attcgacaca
     1081 tgaaatatcc gattttacaa catcaaagat tattgaaaca aaaaagagac aggtagttga
     1141 agaaatagag agacttactg ggctcgatta tagacgtttt acccgatcaa tgttgctagc
     1201 acagggcgat ttcgcggcgt ttttactaac agatgccgga gagcgatcaa tgattcttga
     1261 acagattacc ggtaccggta tttataccga aatttccaaa atggttcatt tagtaaccaa
     1321 ggataaaaaa agtgccctgg ctgaaataca gaatctgctt gatgatgtaa acgtaatgag
     1381 tctggaagag gagaatcaag ctgatgcaaa acttaaaggt cttgcagaac tggcttcaaa
     1441 aacagttgaa aaactggaat ttataaacaa tgctatatcc tggcgccgca accttgccga
     1501 ttttgaacag cgcattcgag aaaccgaaaa aaaagagcag tttgcaaaag aggagcagaa
     1561 gctttttgaa aaagagaaag aacggcttgt taaagggcag agggctgccg aattagaagc
     1621 agattataaa gagctctgtt ctgtgcgttt agagctgcaa aataaaaaaa atagtctgga
     1681 aaatataagt aatgaagaga ttccactgct taaaagtatt gatgattcta aaaaaaaagc
     1741 aaatgattta tcacagaaac ttaaaaatat cagaaatgaa aatgctatac tcttaaaaac
     1801 agttcaggaa gtacgtattc ttgataaccg gattgatgaa aagcgtaaag agtacaaaaa
     1861 tatgattgtg agctaaaaga acaacaaaaa gaactgttaa aaaaagagac acaatttaac
     1921 gagataactg aaaatttaaa taaacaaata aataaaaaaa atgaaattga actggaattg
     1981 aagaaccgag ctaatgatga gcctcttatg cgtgagtgtg atttgctttg tgacaagata
     2041 agtcagatta ccgatttaga acgggaaatc cgggagcaag aagaagagag agaatctgca
     2101 aatagtattc atgaaaagca aaagcaaact ttaaatttgc ttttgcaaaa acagagtaag
     2161 ataatcagag attctgaagc ttataaaacg gatattaaaa agatagctgc tgatatggat
     2221 tctctattag gaataagaca aatagacgat tatcgaactg aacttgaaaa actaaaggaa
     2281 gaaaaatata gacgctccgc tgtgagcgat tatcaaaaag agcgatctaa tctgacaaaa
     2341 ggagaaccgt gtcctctttg tggttcactt gatcatccat acgtttcgaa aaaattgcct
     2401 gatgtttcag aaactgatat aaggctggca gatgtacaaa aaattattgg gcaatatgac
     2461 ctgctggaaa aacaaaaaaa agtttatatt gataatttaa acgaaaagga aaaagatcta
     2521 catggaattg aatctgagat aaaagtattc aatgctaaca gtgaagcata tctgaaaagt
     2581 gttaaaaaaa ttgaagccga tacagaaaag cataagataa aaaagcagaa tcttttaaaa
     2641 aaccttgagc cgatacttgt taatcttggc attaaaattg ttgatcttaa aaatccggat
     2701 aaaatcataa attctttatg cgaacgtagg gatggctaca agttttattg tgatgctttt
     2761 gaactatgtg aaaaagaact taacatactg aagattcaat atggagaatt ggatgccgga
     2821 ataaaagcaa taactgaagg tgttaatcgc cataaagaga agactgttat tttggggagt
     2881 gatggaatgg ctctctcgaa agagagaatg cgaatatttg gtgaaaaaga ttgtgacaag
     2941 gcggaaagcg aagcctcggc tgctatcgag gagtgtgaaa ttcttttgga aaaagagaag
     3001 gataacctta ataccttaca gcttcgttta cagaaaatta aaacttcaat cgaaacatta
     3061 aataaggata ttgcccaatt tgcagaaaga ctagctaatc ttttacaagg tttcaagtgt
     3121 gccctggata aagccgattt ttcgtcagaa gatgattttg taagatgccg tatgagcgcg
     3181 gaagagcgtc gagaagtaga aaagagggga atggatatcc aaaaacagct ggtacaagtt
     3241 ttggcatatt taaaagaact aaaagatgaa tacaataaag agatgtcaaa aaaactgagc
     3301 acatctgatc tgaataaatt gcttgcagac aaagaagaac tttcaaaaga gctaaatgaa
     3361 gttcatgaaa aaaaaggctc ccttcaacaa cgaatagagg ataataacaa agcaaagaaa
     3421 gaacgcaaaa gccgtgaaaa tagacagata aaagctaaaa aagaatacga gctctggtct
     3481 aatcttgatt cattgatcgg ttcggctgat ggtaaaagat accgcacctt tgcacagggg
     3541 ctcacttttg gccttatggt ctataatgca aatattcagc ttagaaaaat gagtgaacgt
     3601 tatttgctgg tgcaaggttc tgataatccg cttgaattaa atgtaataga caatcaccag
     3661 ggcgggatag agagaactac aaaaaatctc tctggtggag agagctttat agtaagtctg
     3721 gctttggctc ttgggctttc ccgtatggct ggtaaaaatg taagaattga ttctcttttc
     3781 cttgatgagg gttttgggac tcttgatgaa gatactttgg atgtagcact tgatactctt
     3841 tcatctttgc acagtgaggg aaagttgatc ggtattattt cgcatgtgga atctttgaaa
     3901 agcaggatat ctgcccgtat agaggtaaaa cccgagactg gaggacgtag cctgcttgaa
     3961 ggtcccggat gtaaaaggat tggatagtag ttacaaaaaa gacctccgta aacggaggtc
     4021 tttaaattgt cataaattgg gcgatgacgg gatcgaaccg ccgacccact gcttgtaagg
     4081 cagttgctct cccagctgag ctaatcgccc gtagattgat tcgttttact ggacttgaac
     4141 actacgttaa aacgcggtgg tacgtcaata tttttttgag ttatcggtga aaaaaccgaa
     4201 cgaccttttt tcttttgcaa gtagaaagta ccaaatcctc gcagatctat ccgttctccc
     4261 ttgcttacct catcaacaat tgcctttaag aaaaggttta cgaccttgtt gatatcctgc
     4321 ttattgagat taatatcttt gtgcacaata ttaaccatat cttttttggt caaaataaag
     4381 cctatgctaa tgcagaatta acttttttcg ccatacgaga cttgaggcga gctgcttttt
     4441 tccaatgtat aacattgttt tttgcaactt tatcaacagt ctttgcaaaa ttattttgaa
     4501 gagccacgac ttcgtctgac ttgatctccg ctttcgcatc aagaagcgag agtaattttt
     4561 ttgaagcagt tcttagcgca gacttgtttt gagaattacg catattgcgc ttagctgctt
     4621 gccggatgcg tttttttgta gaattattat tagccaattt gtaaaacctt caacatataa
     4681 agttcaaaac gttgtgaaca tctattatct ggctatcatt tctgtcaaat aaaaaacctt
     4741 taaaaaatta taaattaccc gaatacggaa ttacagtaaa ttaaaataaa acagaacagt
     4801 ataattctta ctctacttgc ataataattc ttttaaaaag gtcatttgta taaaaataga
     4861 gtctagcttc aaaaccacca gcttttgatc gtatcatgaa aagtgctgtg gtatcacctt
     4921 ttttaaaaga gtgagttgtt gtagtttgct ctctttttgt ttccggagca ccaaaatctt
     4981 tttttaagtc ggaaaaaata gtcataaact gcttttcgtc taagagagtg taacgacatg
     5041 aaatagctga taatctgtta ttcagaaata tgaaatcgcg gtaaatctga tctttttcct
     5101 gataatatat ttccggtttg agtgatccat caaaatcaag accgctaatg tgattgagta
     5161 tgtagttttt gtaggtagag agttctttat ctggaacctt atgaatttga gcattaaaat
     5221 cggcagtttt cccggttatc tgactttgag tcagtgacca gtcacagata gatcttgttg
     5281 ctttgtacag attttcgccc aaaaggagcg taaagttgca gatcaatgca attataacta
     5341 tggtcttttt catggaaatt aatctattac ctgtttgtta tttagtcaac tcataatata
     5401 tgtgttaaca aaaaacttat acgtaaaata atgtggcaat attattatat tgcctccaat
     5461 agtcaagtct gtgattgata agttatcaaa tactaaattg atttgacata gtctgttgcg
     5521 acagttcaat tgatctaggt ttattaaaaa aacgcatatc tgttttacta taagatatgt
     5581 aaaatgggtt tataatggag ttatcatatg atagacagta aattgcttaa tatcctggct
     5641 tgtccggcat gtaagggtga tgtggaatac gatgcaaaag cacagtcaat tcgttgtctt
     5701 gaatgtgcgc ttgtttatcc ggtagttgac ggtattcccg taatgctcat agataaggct
     5761 caaaaaccag aagaagatga agggaaaaaa gagaaagacg cggaatagtg gaaattatgt
     5821 aaaacaggat aaaaccttgt atggtcacaa cttccctctt gacaaataac agaccttttt
     5881 tgtagattta caccgaatgc cggttctatt agggaaaaaa gatcggtagc cggcgtttat
     5941 tgtccgtagg ctgcttttgt ttgcagcttt tttttgtggt acactctgta gtttttatag
     6001 cagatataaa ataatttgag gttcttaaat aaaatggtta aatctaaaaa tatcgtagtg
     6061 aaagtgttta gttatatttt agtaggtatt cttacaattc ttttggttat ttctcttgga
     6121 agcccagggc tcatggataa ggccatgctg aatcagaata ccttggcggc tgttaacggt
     6181 aagaaaattg atgtgcagga tttcggatat ttcctgaata ccgatcagag attttataat
     6241 agagcttcgg aaatcagaga tgatgagcag ctgttaaatt atgctcgttc ggtctatttg
     6301 cagcgtgttt taactataca atacgcgcaa gagattggaa ttgtggttgt cgatgatcag
     6361 gttgttgact atataagttc tcttggaaat tttaaagatg aagacgggaa gtttatgcct
     6421 gatagacttg atcagtttct taaatattac aatatgtcgt ataatcaatt ttttaaaaga
     6481 acttatgatg ttttggtctt aagggagctg gataatctta ttgtaaacgg tacaggtgtt
     6541 tcaccagatg atatacatat ggataaaaca gtaaataatt ccaaaattac acttcgctat
     6601 ggatttcttt ctaatgccga gataaaaaaa cgttatcctg agagattaac aatttctgat
     6661 aaagaaatag atgatgaggt tgagcgtttg aaggaaacca taaccgagcc tattgatgat
     6721 actgaattgc tgaaaaaaca ggcgaaaaac gagcttgaag tccgtaaact ttcagaacta
     6781 aaattagaaa tatcttcaaa gataaacagt ttagcacttg aaggcaagcc cttcacggaa
     6841 gggcttgctg ttcttgatgt taaagcggaa gattccaaac catttaaaat tggtagtccc
     6901 gtttattctg cggaagaatc aaaaggactg ttgcttgatc tttatacaaa cagtatattt
     6961 tacgaagatt gcttaaaggt aaaacagggc gaacttgctc gtgcggttga tgtttcatca
     7021 ggtttgtatg tttttactcc aattgaaaag attgtccctt ttgaaaaaga tggagaactg
     7081 acagcgtatg attcttatat ggctcatcag gagagatcac tttttgtcga aaatgctcta
     7141 ttgtctccat ttatcgagaa gtcccgtgtt ataagaaatt attagtaaaa aaatcatatt
     7201 ttttaaaaaa atatatttct ttacaaattt tttattatat tatagcaaat aacagcagac
     7261 tgaggttttt ttgtacgttt gcctctctgc tgtttgcata attaatcata taggctggaa
     7321 aaaatgaata ttaatgctga atatgtaaat ccctttcttg aagcagctag tgctgtgttt
     7381 aagtcagtta tgaatgttga tcttcgtcgc ggaaaaatga ttatcaaaga aaatcctgag
     7441 ccgtcgcacg atgtggcaat aattataggt atcacaggtg caattaacgg tgaagttgtt
     7501 tacagtatgg gttacaatat ggtaaccaaa atcgcggaaa tacttgcacc gggcatgaca
     7561 gaagagcaga ttaagtctga gtataaggat atcgtggggg aacttgcaaa catgataacg
     7621 ggtaatgcca tgaacctgtt tgcctattcc ggtaaatcaa ttgatattac gacaccaaca
     7681 gtggttaacg gaaaggattt tacaataacc ctcgtgcagc aaacaacgct tggtataaat
     7741 ctatacagcc caatgggtca acttgaaatg aacgttgctt taaaataggc tgatctgatt
     7801 atagatcagc tgttcgtttt tttgtatttc cagtctcttt ttttgctgtc catataatcc
     7861 gagaattcgg cttctgtgtc gaacatatgg gtaaagtagt cgtcttcacc ttcaaattca
     7921 cagcactcat cttttgatgg gtaatccctg caaatttttg gtctactctc gtagcatcca
     7981 caacggttat ctttctgtat catctcacaa tctgtgttaa acttgataaa ccagtcgtta
     8041 ttgtgatcga taaaaactga aacgtttttg tgcaaaagat accagcgtat atagtcatac
     8101 tctttttttg ttgtaggctt gtctatttca acagctagat gtctgcaaca ttgtgccttg
     8161 catttatcgc atggaggact cttttccata ttaatacctt gataatattc agatttttat
     8221 tataatttaa agatggagca taggtaaaag attaataata gccgtggtat ccatataaaa
     8281 cggcattaag ataatcagaa ttttaaaaaa taaaggtttt atgaatcatg aggtttttgt
     8341 aacttctttt gtcaaggtta ctttgtcttt tattaagata tttgtaaaaa ttgagctgat
     8401 tcggataagc attttttgtt ataattaatt ttagtagttt tcgtataatt atattaaaaa
     8461 tataaagtaa tggcattagt tttctggaat gccaaataaa atagtctcat aaatatatta
     8521 attggaaggc gtttgtttga taactgaaag ttggacactt ctttatgacc gtgattgccc
     8581 actttgtgcc cgatttgcgg caattgtaca gaacctggat catgaaaata tgattgattt
     8641 tgtacccctc caggattatt gtaagaagga gatccgtttc tcttgcgagg agttgctaaa
     8701 aacggttcac atcattagtg aacagggtca ggttctaaag gggggggatg ccgtttgtaa
     8761 agtactaatg cttgtacctg catcgcgtcc atacagatgg ttgctgcaaa gcaaagtagg
     8821 gcgccgtgtt tctaacactg tatattttgc tttaaacagc tttagacgtt gctataaatg
     8881 taatcgaaga aggtaaacat gaatattatt atggtttata gtgatgagtt ggttgcgcag
     8941 tctgttgcag cagtaagtgc tgttcatgca atttcaatgg ttaaggctaa tgaaagtgag
     9001 ggtatcgact ttacaaatta ttctcttgtt cttttgaatg ttgatgatat tctctgtgat
     9061 cggttgttgg ataagataaa caaaaaggta ccggttttgc gtcttaacaa cagtaatcag
     9121 tttatcaaga tgatgaatta tgttactaaa ggtcttgtgg gtactgtttc tcttccattg
     9181 tcaggagctt ttttgtacga gataattgat tcgttaagca ggcaggttgt aactcttcct
     9241 gaaaacagta acgacaaata tcaattttac tataatggtg ataaaataga agtatgtgtt
     9301 aatccagatt ttttattaag atctgttcgg atagcggaac aggatataga gcaaaaaaaa
     9361 atagcagtta ctcatattac atctaataaa aaggtcgacg ctgcctcggt aaacgattct
     9421 ctttcacgac ttcaggagga agtagtcgag ggacttgtca acgatgaatt ttcgttagcg
     9481 tatcaacctg ttcactatct gaacgaccag atgttatacg ggtttgaagc ccttatccga
     9541 tggaaccgaa aggggttact tatctcacca gatgatttta tacctcttgt agaaaaaaca
     9601 gatgttgttt atgcgcttaa tgattgggta ttgaatcagg ttttattaga tttagaagat
     9661 tggaatgtta aattcaccac aaatggcgat aatttaagag taaatatcaa tctttcggct
     9721 catctgtttg aatctccagg gatcgcggac aaaataattg aaagtgttaa aactgccaaa
     9781 attgatccga gacagcttgg gttggaatta actgaatcgg catttatgga ttcaaaagag
     9841 attgcaaact tggcactttt aaaactaaag tcgaataatt ttcctattta catggacgat
     9901 tttggaacag gttacagctc tttatcatat ttacaacatt tcccggtgga tattttaaaa
     9961 attgacaaat cgtttgtaaa atggattact ttcgatgaag atagtgagca tatcgttagc
    10021 tcaattattg gacttgcaca tggcttaaaa atgaaggtag tcggtgaagg tgtagaagag
    10081 agagcacaag tagactttct aaaagagaga ggctgcgagt ttggccaagg attctttttt
    10141 agccgtcctc tcaaaaagaa tgacgcatcc ttatatcttg ctgattatca caatagacgc
    10201 tagggggagt atggtacgga tacttgtagt ggatgatcat ccgataatat atcaagggtt
    10261 ggttcagatg cttcaggatg atttgcgatt taaggttgct gacaacgcaa aaacttatga
    10321 tgaagcaatt ataaaactgg aaaaacataa acccgacctt ttgatagtcg atataggtct
    10381 tcgcagcgag aaaaacggga tagaccttgt caggtttgta actgaaaatt atccagaaac
    10441 aaagacaatg gttctttcga tgcacgatga agagatttat tcagtacgat catataatgc
    10501 tggtgcaaga gggtttgtta ctaaatcaga ttttactgag cgaatagtcg aggcaatagg
    10561 aatggttatg aagggggagc tctattatca aaacggtgct ccacttgttg aacataaagg
    10621 ggatcgatta aagcagcagt tctcgaggct taccgaccgg gaaatcgaga ttttacgttg
    10681 cattggaatg gggatgaaga atagtgatat tgccgataaa accggagtaa aggaaaagac
    10741 tattcactcg catcgtttac gtataagaaa caaactgcaa cttgagagta ccgcggatct
    10801 gatcaggctg gcgtcttcgt atatcgatga taatgttatc tgattaaata tattaagaag
    10861 acatgattaa tataaaaaga gttatccggg gataactctt tttatatttt agtgcgaata
    10921 gttgtttcat taaattagac gatacatgga ttttcgcttt ttcctgttgg atcaactttg
    10981 tcggttgcat cgaaaaattt acattttgaa ataacaacac caagcttgac actctgatta
    11041 acgctgaact gctttgtagg atctgcttta agaaccatat tgctgtcaga gtcaaggcga
    11101 acatgaagaa ttgtttctga accaagaggt tcaacaactt ctatcacggc attaattgag
    11161 tttgttgggt tttcttcaaa agtgaaatct tcggggcgaa caccgaaaac aatatttttt
    11221 gagccatact ctttaagttt tgcaagttgc tctggtgtgg gagtaagatt gattcctttc
    11281 tctgtcaatt tatcaccagt caaagaaact gggtgaaagt tcataggagg agtacctata
    11341 aaaccggcaa cgaacatgtt atttggattg ttataaagtt ccataggagt gtcaatctgc
    11401 tgaatgaaac cgtctttcat aacaacaatt cggtcaccca tggtcatcgc ttctgtctgg
    11461 tcgtgagtta cataaaccat tgtagactga agttttgtgt gtagacgtga aatttcagca
    11521 cgcattgtta cacgaagctt tgcgtcaagg tttgaaagtg gctcatcaaa aaggaaaacc
    11581 tttggatgac gtacaattgc gcgacctaag gcaacacgct gacgctgacc accggaaagt
    11641 tgtttaggtt tgcgatcaag aaggttttcg atgtgaagaa tctgagctgc ttctttaacc
    11701 tgtttttcta taatatcttt tggagttttt ctcattttaa gaccgaaggc catattgtca
    11761 taaactgtca tgtggggata aagtgcatag ttttgaaaaa ccattgcgat atcgcggtct
    11821 tttgggtgaa tatcgtttac acgtttgtca tcgatataaa ggtctccgct tgtgatctct
    11881 tcaagaccgg caagcattct aagagttgtt gatttaccac atcctgaggg tcctacaagt
    11941 attacaaatt ccttgtcctg gatctctata ttagcgtcgt aaaccgcttg aaaaccgttt
    12001 ggatatatct tgttgatatc cttgagtact accgttgcca taaaatctcc ttcctactta
    12061 attttttggt ttagtggtat ttcccgttta agaattattt aactgattgt cacgcccgca
    12121 aaatatctgt aatgtatgca gatattaatc aaaaacggat aacaaatagt aaattaaaca
    12181 ggaaaaactg ataatataga agaaagtgtt ttcttctata ttatcagttt ttctgattat
    12241 tcggtcaaac tgtcaactat ttttcatttt attaatttaa tatttgttga agatagatat
    12301 cgggtatttt ataaaacatg tgttatatct atatttttat gaaattagtt caagattacc
    12361 tttacataaa ttgagtattt tcggataagg tttgtaatcg gtgaacatat ctattagtct
    12421 tattcttgct ccataattac tcttgatcgt ttcaagttgt attataatta attatacgta
    12481 acgcgatatt taaaaattgc ggttattttt aacggagatt cttatgaaaa atattaaaaa
    12541 aatatcatct ttaattatag cgatgttaat agttctttgt ggttgctcaa aaaattatga
    12601 cccaatcaat gttgggctgg ttttgacagt ggagggccca ggagatgaat cgtttaacga
    12661 taattccttg gctgcgttag aacgcgccaa attggattat aaaatcaatt atgattataa
    12721 agtaccgact tcgatggcag atatagataa tacaattgaa tacttcgcaa agaataatta
    12781 tgatttggta atcgtaaccg gatttttgat gcacgatgct tgcgagagta ttgctgataa
    12841 gtatcctgaa actgatttta taattattga ttcacttgta aagcgtccca atgttaaacc
    12901 tatattgttt aacgagaaag agggggcttt tgttgcaggt gctgttagcg ccttagtttc
    12961 acgttccggc aatgtgggtt tcattggagg tatggatctt ccttttataa aggagatact
    13021 tgaaggtttt agagccggtg tgcagcatca aaataccaag attcgagttg cctctgtcta
    13081 tttaaatggt gaaaacccgt tcaatgatcc actacaggct aacaaagagg ctgaaaagat
    13141 gattaacgaa ggtatagata ttattttcca tatcgccggg aatagtggta ttggcatagt
    13201 taaggcctgt agtgaaaata gggtgtttgc aattggcgaa aaaggtcagt atggaagttc
    13261 cgatgatact attcttgcta atattgtaaa gaatcctgaa aatgctgttt acaggttagt
    13321 tgttgaaagg ctaaagggcg aatttgataa cgcaccggtt ttatccggtt tctctgaaaa
    13381 tggcataagt atcgattttt atccttataa aagcggaaat gatgtaagta atttacagga
    13441 aactgtacaa gctgcacaag aagctctatt atcaggcaag atttccatag agtaatgttg
    13501 ttataagggg atagtaatct tcggttattc atgctaatta taagaaaagt gagtttacgg
    13561 atttatctgg ttatacactt ttttagtttg attttgaata aaaatttatc tatttttaga
    13621 aaagtcaaaa tcaataaggt gtaatatgtt atccagatat aaatcgttaa tatgttttct
    13681 gcttctttat ctgtttggtt tgctttccgg tttttttata aaaaccggtg tgtcaaatta
    13741 taagcaactc tcttctctgg cttttttaat tatacctttg ctgattttta tcatcttctg
    13801 tatttttgta atccttatta gtagaagcag agcggttaag ctctatctta ggctcattgc
    13861 cctgataaga tcccttcccg acatacatat acactcactt tctgatgcaa aaaatattag
    13921 caatatgttt gccaaaatgg gctatttcga gcagataatc tccgtatatc agagcaagat
    13981 cagggctcga taccggcatc tggattctaa tagtatgatt cagacagttc ttctaagcct
    14041 gaataacctt catatcgaga tggataaccg taatgagttt tttaaagctg ttttggaaag
    14101 cgcaataact gttatacctc aagccgacag tggagctctt cttgtacttg attgtgatgg
    14161 tattttacac tacgaggctg ttatcggttt tgatattgcc aatgtgtcac ctgtcgaatt
    14221 aaaaaaaact gaaatataca aaagaaacaa aactcatata tttgagccat gtataataag
    14281 atcggcattt tctgataatg atagagggga aacgcaagat tttcattcag ctgtttgtgc
    14341 tccaattaac atcgaaggga atctgtttgg agttttagta atcaacagta ttgacgagaa
    14401 tgcgttcggt gaggatgatt tagtgctaat aagc
//
LOCUS       DAOTGZ010000049        14178 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_3064
            NODE_5145_length_14178_cov_5.344474, whole genome shotgun sequence.
ACCESSION   DAOTGZ010000049 DAOTGZ010000000
VERSION     DAOTGZ010000049.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868088
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 14178)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 14178)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:22:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,785
            CDSs (total)                      :: 2,751
            Genes (coding)                    :: 2,732
            CDSs (with protein)               :: 2,732
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 12 of 19
            Pseudo Genes (internal stop)      :: 5 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14178
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5145_length_14178_cov_5.344474"
                     /isolate="AMR_MDS_3064"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1275
                     /gene="uvrA"
                     /locus_tag="P5123_04955"
     CDS             <1..1275
                     /gene="uvrA"
                     /locus_tag="P5123_04955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002973764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009380 - excinuclease repair complex
                     [Evidence IEA];
                     GO_function: GO:0009381 - excinuclease ABC activity
                     [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrA"
                     /protein_id="HRX15639.1"
                     /translation="PSIGLHQRDNRKLISTLKKLRDLGNTVIVVEHDEETMMEADYVV
                     DLGPGAGEQGGYIIAQGTPKEIIADKNSITGQYLSGKRSIAIPNNRLIPQNFIDIKGA
                     TGNNLKNIDVSIPLGVFTCVTGVSGSGKSTLIMQTFYKALSNLIMRSKQIPCHFDKIT
                     GYKNIDKVIDIDQSPIGRTPRSNPATYTGIFTPVRELFAATQESRIKGYKPGRFSFNV
                     AGGRCESCEGDGVKKFEMHFLSDVYITCEVCKGKRYNHETLEVRFKGKNIFEILEMTV
                     NQAQEFFENVPSIKRRLDTLVDVGLGYIKLGQSATTLSGGEAQRIKLATELSKRSTGK
                     TLYILDEPTTGLHFADIEKLLKVLRSLVEKGNSVLVIEHNLDVIKTADFVIDMGPEGG
                     DKGGFVVATGTPEEIIGVKESYTGQFLKKILG"
     gene            1284..3308
                     /locus_tag="P5123_04960"
     CDS             1284..3308
                     /locus_tag="P5123_04960"
                     /inference="COORDINATES: protein motif:HMM:NF012321.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HRX15640.1"
                     /translation="MRNRSLRKAFLLSLVFAAFLPLVSVGQGSVSYEIARDHFKKGVI
                     LFNNYSYLGSVEFFRTALTVYPEYHQAREYLARAYRLAGYTDAALREWEYLFDISNDA
                     SVQNKIDMLRYHRVYPMQPPMEPEFTHVKNLESRELRSYHFRYPTDTSVDYEKNIYIV
                     SFNSGRVVKINDSGEGLDTYKPALESRLYGLDIKDNFLLFSDFARDIVYCTDLNFNEE
                     FSFGGSGSGDGQFRGPQGVCFDDKSGIFVVDSGNHRVQHFSKEGKFILQFGKKGNYDG
                     EFQNPTDVAFLDELVYVTDTGNSRVTVYDTSGNFIRNITNMAIKSPRGISVNRDKLIV
                     SDSLEGLIIINPKNDSLTVFDSWGENNQNGFSRLYSAVFDRDDFLYALDHDKERLSVF
                     SPVQKRYTNLDIEIKSVDISNYPTVAFYVSVRDRTGDPVYALKSEEFHVTEDSARIQS
                     VSIDYLKDKPASSTMIFCVDRSEGMDQWKEELVWSADFILTEMKKSDSVKVFDFSDDY
                     ITANDYDWSRRRIQKALRDGIYGGGKNIGRVIYNAVSELLKTDSKRAVVLFTDGIVSE
                     DSFEQYSENIIIDYARCHYVPVYIVSLSGSNPVLRDICDKTGGMFIEARKVSDLRMIY
                     QLIRKQEEYRYVVVYKSLRKPHYTGWWSNVSIEVESKGERGVEWGGYYIP"
     gene            complement(3327..4289)
                     /locus_tag="P5123_04965"
     CDS             complement(3327..4289)
                     /locus_tag="P5123_04965"
                     /inference="COORDINATES: protein motif:HMM:NF017280.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetylxylan esterase"
                     /protein_id="HRX15641.1"
                     /translation="MASLINFDKYFANFPQIERETDFDFFWKNSLIDLKRIAIEPVSH
                     ENTRLSSPRFTVMDIAYKSFGKTSVQARLYMPKKRKHPRPIIIIHDYLEATAFKGYKL
                     ETDFAYLFLTLRGHQILKGIDFHNQKKGFEVESPGYMIENIMDPENYYLKGVYLDCYR
                     AIDLLRLYKDIDCSSIGIIGKGIGGAAATFCASSSKRISAVVLDSLSFADLPKSQNLA
                     DGPVTTEINDFVSSARGKRKILRRNLSYFDAVHFCDRFEIPLLMTTGIKSVTAPAECI
                     FSFFNASVSCDKTMEIYPDDGESAGGAHQFKKSLSWLKKMVYIK"
     gene            complement(4306..4758)
                     /locus_tag="P5123_04970"
     CDS             complement(4306..4758)
                     /locus_tag="P5123_04970"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRX15642.1"
                     /translation="MPNPNREDSPKIALYSTSAYERRPLFNRLKEIIYLSFFVIMSAV
                     VSLIVMNLVTYPILIIVQKGTKYFTDIFILSVIALISLYVLTKLVSTIRYYIASGLSA
                     TQIIPQIIVGRIKALIIFITSLVFISMLIIGLYSLFSYNTRLIINLFS"
     gene            complement(4742..5974)
                     /locus_tag="P5123_04975"
     CDS             complement(4742..5974)
                     /locus_tag="P5123_04975"
                     /inference="COORDINATES: protein motif:HMM:NF025030.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HEAT repeat domain-containing protein"
                     /protein_id="HRX15643.1"
                     /translation="MKYYLMVLLSVSLLYTSLYCQETEKKDTPAEKSSTEAATDDKAE
                     GKTETIKDESAATKDKDVEISDKQRENDIKGAAYIRKTLKFGMQKERAIAIRNIDRIK
                     TPSLKKELIDEVINNLDDEKNTSVIINTINLASREKRKDAASKLTPFLSHPENDVKIA
                     AVYAIKDLEAKETAPELIKELKSQDFSVNSSFIEGLLVALGTFEEKSLYDFVKKTIED
                     PDTTPFHRLRLILFLGDSKTNAAAPYLKEVFSNEDEDLSLRSYAVSALSRMDEKSAIA
                     DINKEIEKIDSFPFKKKQRYYNFYIYCVTSLVKLGDPKAYPRLEESLKSDNAAVRLQS
                     IQLMKDLKDKRSIDILKYKAEYDQNSKVRNEAKKVLKEQFDVDLDKDKKSDKDKKEDS
                     IVDDPGETMDTEEDAESE"
     gene            complement(6077..7525)
                     /locus_tag="P5123_04980"
     CDS             complement(6077..7525)
                     /locus_tag="P5123_04980"
                     /EC_number="2.4.1.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR02095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004373 - glycogen (starch) synthase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycogen/starch synthase"
                     /protein_id="HRX15644.1"
                     /translation="MSKMTLVIASAEASPFVSTGGLGESVGTLVSSLASDAVDIFLFL
                     PRYRSINLQDFKAKKEKTALTVNPGVTSRTAALYVSNHIPGVKTVFIEHNELFDREGI
                     YGQDGMDYSDNCARFSFFSHAVISALPILKIEPDILMCHDWHCALIPLLTKTIYTGLK
                     QLESAKSVFTIHSMDYQGIFPATDIIWTGIGWDGYTDDNLKFKDSINLVKSAILWSDA
                     VITLSDNYAIEICDNHENPLSTLLYDKGVTGIALGIDYDSFNPASDKLITTRFHVNDM
                     VGKESCKSALQRNAGLPLDSDKPLMSLFGPLTFSKGMKIFADAVGPALNQLDFQCIVC
                     GMGDLIIEQQFEYLKKIFPGKFSYIKNVDQKMFRTLIAGSDFFVIPSTNEPYQLNTLY
                     AARYGAVPLLTQRGSLKDNMSCWDSISEDGVCISTPHIDEFSINQLIISSIKTYSTPD
                     FVSLQQNTMKIQYSIQKSAQRFITLFKNLINK"
     gene            complement(7527..9179)
                     /gene="gpmI"
                     /locus_tag="P5123_04985"
     CDS             complement(7527..9179)
                     /gene="gpmI"
                     /locus_tag="P5123_04985"
                     /EC_number="5.4.2.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014624177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046537 -
                     2,3-bisphosphoglycerate-independent phosphoglycerate
                     mutase activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-bisphosphoglycerate-independent
                     phosphoglycerate mutase"
                     /protein_id="HRX15645.1"
                     /translation="MLKLDKNSKFTPRKGPVVFIIMDGVGNAPEYPGNACLLARTPTL
                     DALKNKYLNTEIKAHGTAVGLPSDDDMGNSEVGHNALGAGRVFDQGAKLVEKALLSGD
                     IFTTDSWKEVVQKPITDNTTLHFLGLLSDGNVHSNISHLFKMLERAADEGIKKVRVHI
                     LLDGRDVPETSGLTYVQALEEELQKLNSRGLDFRIASGGGRMITTMDRYGADWRIVER
                     GWQAHVLGEARPFKTAEEAIETFRSEKAGLADQFLPSFTIVDENGPVGTIEDGDSVIF
                     FNYRGDRSIEITQAFEQKDFDKFDRKRFPDVVYAGMMEYDGDLHLPKNYLVQPPAIDK
                     SISEYLASAGVKQFAISETQKFGHVTYFWNGNKTGKFNEETEVYKEIPSDLVPFEERP
                     WMKAAEITDAVIEAIESGEYPFIRLNFANGDMVGHTGHLEAAITAMQAVDLSIERLLP
                     VIEKAGGIAVITADHGNLDEMWEMDKNGNILYDKNDKPKKRTAHTLNKVPFIIFDPQY
                     SGEYTLSKLESEAGLGNVAATLLNLLGFEKPEEYNPSLITFK"
     gene            9470..9697
                     /locus_tag="P5123_04990"
     CDS             9470..9697
                     /locus_tag="P5123_04990"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRX15646.1"
                     /translation="MNSLRNGVSFLVCIAGAFFAIAGVVSMITRFIGYEVKIFLWLSE
                     FETVTAWLIRAGVIAGGILVYVFGVLIDPER"
     gene            9798..13010
                     /locus_tag="P5123_04995"
     CDS             9798..13010
                     /locus_tag="P5123_04995"
                     /inference="COORDINATES: protein motif:HMM:NF013069.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HRX15647.1"
                     /translation="MFGKIIEWFSHRHLLVNIIAISVILGGIIAWDNTKKEEMPDITF
                     PFSRVMVVWPGASPEDMDRYVTVPIEKALRGIDGIEEISSTTSQGSTILSLWLPAKAS
                     ESEMEDIVQDIKDAIDRVDLPDDIKDDPEVRVPNTTRFSVINISMYRESAKLFSNEDR
                     KKVQQYARNLENSLLSLTSVSEISWRNYSVPQVNIYLDIAALNELNVSISEAISAIQN
                     GSLRSPVGTMKGEWSRSEVKEDKRPEEETRSITIEKKSSKQSEFKDYKIIMDAELTKV
                     DDLENLIIRMNFEGKAVYLKDIARVVPEFEESSAILKTNGYEAVTINVTKSSSAGIID
                     AVDEIRKKVEEFKKNSLAGTDINVVLVDDESIAVKDRLSMVSGNGILGFVLVVIFLFL
                     FLNAKSGVWVAFGLPFSLCFTLIGMYMLGYSINNTTLAAVIIVLGMVVDDAIVVAENI
                     NRLEASGMPRKQAVVEGTGRVFLPIVASIVTTCVAFIPLFFFGGRFGVLNAFIPPVIF
                     IMLFASLFESVFILPAHMSSSLFDSKKGATERSWFIKLEEGYQQILRFLLAHRKPVFV
                     FAIILLFFAGFLFTTTMKYEMFPREESTQLRIEGEVAYGTLKKSTARKTEEVEAIIDE
                     YVGNEALSYETRIAQTRRSNAARENVFSIMIELVPLEKRDRKSSAIQKEMEQKLKRLK
                     GFEKLEFSQGRFGSTSGNPIEVHVRDNNDETRNKLTRELLQAMKNYAPYINVDEERQL
                     QDPEYLFKPKRTIAGSLNVSISRILSALRTAINGTILYEVPENGDDVEYRITVEDKDR
                     SNLELLRNLPVLNNSNYSIPLGNVVDVIEGNSANTISRMDNVRTTRIFSDIDASSGLT
                     PVETAIYFEENIFPDLLSRYPTSSIVYAGEVKDTRQSTGEFIYSVIAVLLLIYIILAL
                     LFDSLVKPFIIMATIPFAAVGIIFALKLHGISVYGFFSVVGCLGLIGVVVNDSIVMIS
                     VLEANYKAHPNIKKAIVAISEIAKTRLRAVILTTITTVAGLLPTAYGILGYDSMLSDM
                     MLVMAWGLIFGTIITLVFVPILYLIMYRIQHHVKDIANSN"
     gene            complement(13113..13562)
                     /locus_tag="P5123_05000"
     CDS             complement(13113..13562)
                     /locus_tag="P5123_05000"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRX15648.1"
                     /translation="METTDFRTSIDVEISRLDKIEEFANKHNMHISDVINTVLDKYSK
                     MLAKNPVCNDSLNKYHERGTIYKSVHIRFSSISKRKLKDVANLYCYSLSYMLFLAMNF
                     FDEFMSSVKEFIRPCFHAVFVHNFFGFPYFINSWGMNFYDSHDYSSG"
     gene            13775..>14178
                     /locus_tag="P5123_05005"
     CDS             13775..>14178
                     /locus_tag="P5123_05005"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRX15649.1"
                     /translation="MIKTVFTRLYILFLLLVILTISTSCYNQANISINIVEKQKRRLT
                     AELTINMKNNESLDINDIKIKYFIDNDFIEIKKNLAKDSVNVDYLNIDKIDVREFSVT
                     SKMTLEKDKALINFMKFTSDLDKNNKYNCPLIL"
BASE COUNT         4416 a         2327 c         2820 g         4615 t
ORIGIN      
        1 ccttcaatcg gattacatca acgggataat cgcaagttga tatcgacatt aaaaaaactc
       61 agagatctgg gtaataccgt aattgttgtt gaacatgatg aagaaaccat gatggaagct
      121 gattatgttg tggatcttgg accgggtgcc ggtgaacagg gtggatacat tattgcgcag
      181 ggaactccaa aagaaatcat tgcggataaa aattccatca ctgggcaata tctgtctggt
      241 aaacgtagta tagccattcc gaataaccgt cttataccac aaaatttcat tgatattaag
      301 ggagcaacgg gaaataatct aaaaaatatt gatgtttcca ttccattagg tgtttttacc
      361 tgcgtaaccg gtgtttccgg atcgggtaaa tcgacattga taatgcagac tttttataaa
      421 gctttatcaa accttataat gcgttcaaaa cagataccct gccattttga taaaattaca
      481 gggtataaaa atatagataa ggtgatagat atagatcagt caccgattgg acgaactccg
      541 cggtcgaacc cggcaacata tactggaata tttacaccgg tgcgtgagct ttttgccgca
      601 actcaagagt cgcggataaa gggttataaa cctggaagat tctcttttaa tgttgccgga
      661 ggacgctgtg aaagctgcga aggtgacggt gtaaaaaaat tcgaaatgca cttcttatcc
      721 gatgtatata ttacttgtga ggtttgcaaa gggaaaagat ataatcatga aacgcttgaa
      781 gtacgtttta aaggtaaaaa tatctttgaa attcttgaga tgactgtaaa tcaggctcaa
      841 gagttttttg aaaatgtgcc gtccataaaa aggcgccttg acaccttggt agatgtcggt
      901 ttaggatata ttaagttggg acagtctgcc acgactcttt cgggcggaga ggcacagcgt
      961 atcaaattgg ctacagagct atcaaagaga tcgaccggaa aaacactcta tattcttgat
     1021 gaaccaacaa cagggctaca ttttgccgat attgaaaaat tgttgaaagt tttacgctct
     1081 ctggtagaaa aaggtaacag cgtacttgtt attgagcata atctggatgt gataaaaact
     1141 gcggattttg ttatcgatat ggggcccgaa ggcggtgata aaggcggttt tgtagtggct
     1201 acaggaacac cagaagagat tatcggtgtt aaagagtcat atacgggaca gtttttgaaa
     1261 aaaatactgg ggtaagatga taaatgcgaa atcgatcttt aagaaaagct tttctactat
     1321 ccctggtttt tgcagctttt ttaccgttag tttctgtagg tcagggcagt gtctcttacg
     1381 aaatagctcg tgatcacttc aaaaagggtg taatactctt taataattac agttatttag
     1441 gttcagttga atttttcaga actgctttga ctgtttatcc cgagtatcat caggctcgag
     1501 aatatttggc aagagcctac cgcctagcag gatacacgga tgctgcgtta agagaatggg
     1561 agtatctttt tgatatttct aacgacgctt ctgtccaaaa caagatagat atgctccgtt
     1621 atcaccgtgt ttatcccatg cagccaccaa tggaacctga atttactcat gtaaaaaatc
     1681 tggaatcccg cgagctacgc agttaccatt tcagatatcc taccgataca tctgttgatt
     1741 atgaaaaaaa tatctatatt gtctcgttta attcaggaag agttgttaaa ataaacgatt
     1801 caggagaggg actggatacc tacaaacctg ctcttgaatc gcgtctttac ggtcttgata
     1861 taaaagataa ctttttactt ttttcagatt ttgcaagaga cattgtttat tgtaccgatt
     1921 tgaacttcaa tgaagaattt tcttttggtg gatcaggatc aggtgatggg caattcagag
     1981 gtccacaagg ggtttgcttc gatgataaat cgggaatatt cgtagttgat agtggtaatc
     2041 accgtgtaca gcatttttca aaagagggaa aatttatatt gcagtttggc aaaaaaggaa
     2101 attacgatgg agagtttcaa aatccaacgg atgtcgcctt tttggatgaa ctggtttatg
     2161 taactgatac aggtaattcc cgagtaaccg tatatgatac atctggtaat tttatacgta
     2221 atattacaaa tatggctatt aaatctccac ggggtatttc tgttaatcgg gataaactta
     2281 ttgttagtga ttcattggaa ggtcttataa taattaaccc taaaaatgat tctctgacgg
     2341 tgtttgattc atggggagaa aataaccaaa acggattttc aaggctatat tcagctgttt
     2401 ttgacaggga tgattttctt tatgcacttg atcacgataa ggagcgattg tccgttttct
     2461 cacctgttca aaaacggtat acaaatcttg atatcgaaat aaaatctgtt gatatttcga
     2521 attatccgac agtggctttt tatgtgtccg ttcgtgatcg caccggtgat ccggtctatg
     2581 cacttaaatc tgaagagttt catgttacgg aagacagtgc aagaattcag agtgtctcga
     2641 tagattattt gaaagataaa ccagcgagtt cgaccatgat tttttgtgtg gatcgttctg
     2701 agggaatgga tcagtggaaa gaggaacttg tttggagcgc ggattttatt cttacagaga
     2761 tgaaaaaaag tgattctgta aaagtgtttg atttttcgga tgattatata accgcaaatg
     2821 attacgactg gtctcgtcgt cgaattcaaa aggcacttcg tgatggaata tacggaggcg
     2881 gtaaaaatat aggacgggtt atatataatg ctgtatctga acttttaaaa acagattcta
     2941 aacgtgcggt ggttttattt acagacggaa ttgtttctga agattcattt gagcagtaca
     3001 gtgaaaatat cattattgat tatgcacgct gtcattatgt tcctgtatat attgtttctc
     3061 tatcaggaag caatccagtt ttgcgggata tttgtgacaa aaccggcggg atgtttatcg
     3121 aagcacgcaa ggtatcagat ttgagaatga tttaccaatt aatacgtaaa caggaagagt
     3181 atagatacgt agtggtttat aagagtttga gaaaaccgca ttatactggt tggtggtcaa
     3241 atgtatcgat agaagtagag tcgaaaggtg aacgtggtgt tgagtggggt ggttattata
     3301 ttccttaaga cggtataatg gataaattat ttaatatata ccattttctt tagccatgat
     3361 aggctttttt taaattgatg agcgcctcct gcgctttccc cgtcatcggg ataaatttcc
     3421 atggttttat cgcagcttac agaagcattg aaaaaagaga aaatacattc agctggggcg
     3481 gtaacagatt taataccagt ggtcatgagc aaaggtattt cgaatctgtc acaaaaatga
     3541 actgcatcaa aataacttaa attgcgacga agtatttttc ttttgccacg cgcactgctc
     3601 acaaaatcat ttatttcagt tgtaactggg ccgtcggcta agttttggct tttaggtaag
     3661 tcagcgaaag aaagtgagtc tagaacaacc gcactaatac gttttgaaga ggatgcacag
     3721 aaagtggctg cggctcctcc gattcctttg ccaattatac ctatagagga gcagtcaata
     3781 tccttataca atcttaacag gtcaattgca cgataacagt caagataaac tcctttaagg
     3841 tagtaattct cgggatccat aatgttttcg atcatataac ctggggattc gacctcgaat
     3901 cctttcttct gattatggaa atcaattcct ttaagtatct gatgaccacg aagagtcaaa
     3961 aaaagatagg cgaaatcagt ttcaagttta taacctttaa acgctgtggc ttcaaggtaa
     4021 tcgtgaataa ttattattgg ccgcggatgt tttctttttt ttggcatata gagtcttgct
     4081 tgtaccgaag ttttaccaaa acttttgtat gcaatatcca taactgtaaa gcggggagat
     4141 gaaagccggg tattttcgtg agaaaccggt tctatagcaa tacgttttaa atctataagg
     4201 gaattcttcc aaaaaaaatc aaaatctgtt tccctctcta tctgagggaa atttgcaaaa
     4261 tatttatcaa aatttataag agaagccatt aattcgttac cttaattatg aaaagagatt
     4321 gattattagt cttgtattgt acgaaaacag tgaatagaga ccaattatta gcattgaaat
     4381 aaaaaccaag gatgtaataa aaataattaa tgcctttatt ctacctacga ttatttgagg
     4441 aattatttgt gttgccgaaa gaccactggc gatatagtat ctgattgttg aaacgagttt
     4501 cgtgagtaca taaagggata taagcgcaat aactgataat ataaatatat cggtaaagta
     4561 ttttgtgcct ttttgtacaa taattaaaat tgggtaggtt acaagattca taacaattag
     4621 cgatacgacc gcagacataa taacaaaaaa tgataaataa attatctctt ttaatctgtt
     4681 aaaaagaggt ctgcgctcgt aagcgcttgt ggaatatagt gctatttttg ggctatcttc
     4741 cctattcgga ttcggcatcc tcttccgtat ccatggtttc gccagggtca tcgacgatag
     4801 aatcttcttt tttgtcctta tcagactttt tatctttatc gagatcgaca tcaaattgct
     4861 cttttagtac tttttttgct tcatttctaa ctttactatt ttgatcatat tccgccttat
     4921 actttaaaat gtcaattgat cttttatctt ttaaatcttt cataagctgt attgattgaa
     4981 gtctaactgc agcattatcg ctttttagtg attcttcgag tctcggatag gcttttggat
     5041 ctcccaattt tacaagagag gttacacaat agatgtaaaa gttataatag cgctgctttt
     5101 ttttaaaagg aaaagagtca attttttcaa tttctttatt aatatccgca atagcacttt
     5161 tttcatccat acgtgaaagg gcgcttaccg cgtatgatct tagacttaaa tcttcgtctt
     5221 cattagaaaa aacttctttt agatatggag cagctgcatt ggttttactg tctcccagaa
     5281 acagaataag tcttaacctg tgaaatgggg ttgtatctgg atcttcaatc gtttttttta
     5341 caaaatcata aagtgatttt tcttcgaagg ttcctagtgc aacaagaaga ccctctatga
     5401 aactggaatt gaccgaaaaa tcctgagatt tgagctcttt tatcagttca ggagcggtct
     5461 ctttagcttc aagatctttt attgcgtaaa ctgctgcgat tttaacatcg ttttcaggat
     5521 gagaaagaaa tggtgtaagt ttagatgctg cgtctttccg tttttcacga ctggcaagat
     5581 taattgtgtt aattataacc gaggtgtttt tttcatcgtc aagattattt attacttcat
     5641 caattaactc tttcttgaga gagggggtct taatacggtc aatattgcga atagcgatag
     5701 ctcgctcttt ttgcatgccg aatttcaggg ttttacgaat ataggcggcc ccctttatat
     5761 cattttcacg ctgtttatca gaaatttcta catctttatc ctttgtagca gcggactcat
     5821 cctttattgt ttctgtttta ccttctgctt tatcatcagt tgcggcttcg gtactgcttt
     5881 tttcagccgg agtatctttt ttttctgttt cctgacaata aagagaagtg tacaaaagtg
     5941 aaacggataa aagaaccatt aaataatact tcatacattc tccctgtagt attctgtata
     6001 aaaaatatca cactcgttat atcggcaaat atgacataat acttaagtaa tgtaaagtcg
     6061 ataataacaa gcaaatttat ttattaataa ggttcttaaa aagagttatg aagcgttggg
     6121 cagatttttg aatagaatat tggattttca tcgtattttg ttgtagtgac acaaaatcag
     6181 gagttgaata tgtctttata gaggaaatga ttaattgatt tatagaaaat tcgtcaatat
     6241 gtggtgttga aatgcagacg ccatcctccg agatgctgtc ccagcaagac atgttgtcct
     6301 tgagagatcc ccgctgggtt aataggggta cagcaccgta tcttgccgcg tataatgtat
     6361 taagctgata aggttcgttg gtagacggaa taacaaaaaa atctgaaccg gcaataagag
     6421 ttctaaacat tttttgatct acattcttta tatacgaaaa tttacctgga aagatttttt
     6481 ttagatactc aaactgttgt tcgatgatta aatcacccat tccgcatacg atacattgga
     6541 aatcaagttg gttaagtgct ggacctacgg catccgcaaa tattttcatc ccttttgaaa
     6601 aggttaatgg accaaagagg gacattaatg gtttgtcaga atcaagtgga agacctgcgt
     6661 ttcgttgaag agcagattta caggattctt tcccaaccat atcattaaca tggaagcgag
     6721 tggtaattaa tttgtcgcta gctggattga aactgtcata atcaatgcct agtgcaatgc
     6781 cagttacgcc tttgtcgtac agtaaagtag ataatggatt ttcatgatta tcacatattt
     6841 caatagcata attgtcacta agtgtgataa ccgcgtcgga ccatagaata gcgcttttaa
     6901 caagatttat tgaatctttg aattttaaat tatcgtccgt atatccgtcc caacctatgc
     6961 cagtccagat aatgtctgta gcaggaaaaa ttccttgata gtccattgaa tgaattgtaa
     7021 aaaccgactt tgccgattca agctgtttta aaccagtata tatagtttta gtaagtaaag
     7081 gaattaatgc acagtgccaa tcgtggcaca ttaatatatc aggttcaatt tttagaattg
     7141 gcaatgccga aataaccgca tgggaaaaga atgaaaatct ggcacagtta tctgaatagt
     7201 ccatgccgtc ttgtccataa ataccctcgc gatcaaacag ttcgttatgc tcaatgaata
     7261 cggttttaac acctggaata tgattggaaa catataacgc cgcggttcgg ctagtaactc
     7321 ctggattgac agtcaaggcc gttttctctt tttttgcctt gaagtcttga agatttatcg
     7381 aacggtaacg aggcaaaaaa agaaagatat caacggcatc actggccaac gaagagacaa
     7441 gtgtgccgac tgattccccc aacccccccg ttgaaacaaa aggggaggct tctgctgatg
     7501 ctattacaag agtcatcttt gacattttat ttaaatgtta taagagacgg gttatactct
     7561 tctggctttt cgaaccctaa tagattaagg agtgtagcgg caacattgcc aagaccggct
     7621 tcactttcaa gttttgatag agtatattcg cccgagtatt gaggatcaaa tataataaat
     7681 ggtactttgt ttagtgtgtg ggctgttctt tttttaggtt tatcgttttt gtcatacaga
     7741 atatttccat tcttgtccat ttcccacatt tcgtccagat taccgtgatc tgctgtgatt
     7801 accgcaatac caccggcttt ttcgattacg ggaagaagtc gctctattga tagatcaact
     7861 gcctgcatcg cagtgatggc tgcttcaaga tgaccggtat gaccgaccat atcgccgtta
     7921 gcaaagttta aacgtataaa aggatattca ccgctttcga ttgcttcgat aacagcatct
     7981 gttatttcgg ccgctttcat ccaagggcgc tcttcgaaag gcacaaggtc agatggaatc
     8041 tctttgtaaa cttcggtttc ttcgttaaat tttccggttt tatttccatt ccagaagtag
     8101 gttacatgtc caaatttttg ggtttcagat attgcaaact gttttactcc ggcagaagca
     8161 agatactcag aaattgattt atctattgct ggaggttgta caagataatt tttaggtaaa
     8221 tgaagatctc catcgtattc catcattccg gcataaacta catcagggaa acgctttctg
     8281 tcaaacttgt caaaatcttt ctgttcgaat gcttgggtga tctcgataga gcgatctcca
     8341 cgataattaa aaaagataac tgaatccccg tcttcgattg ttccaacagg accgttttca
     8401 tcaactatgg taaaagatgg aagaaattga tcggcaagac ctgctttctc agagcggaaa
     8461 gtttcaattg cctcttctgc cgttttaaaa ggacgagcct cgccaagaac atgtgcttgc
     8521 catccgcgtt cgacaatacg ccaatcggca ccatatctgt ccatggttgt aatcatgcgt
     8581 ccgccaccag aggcaatacg gaagtccagt ccgcgcgaat ttagtttttg taactcttct
     8641 tcaagagctt gtacataggt taaccccgat gtttctggca catcacggcc atcaagaagt
     8701 atatgaacac gtaccttttt aataccttcg tctgccgcgc gttcgagcat cttgaatagg
     8761 tgagatatat tcgagtgtac gtttccatct gatagcaggc caagaaaatg aagagttgta
     8821 ttatcggtaa taggtttttg aacaacttct ttccatgagt cagttgtaaa aatgtctcct
     8881 gacaaaagag ctttttcgac aagttttgct ccttggtcaa aaacacggcc agcacctaaa
     8941 gcgttatgcc caacttcgga attgcccata tcgtcatctg atggaagtcc gacagctgtt
     9001 ccatgagctt taatttctgt attaagatat ttatttttta aggcatcaag agttggagtg
     9061 cgagcaagaa ggcaggcatt accaggatat tcgggggcat tgccaacacc atccattatt
     9121 ataaatacta caggtccctt acgaggtgtg aatttgctgt ttttatctaa ttttaacatt
     9181 tttcctccgg aaatgtgagc gataatttcc atacaaaagt atgcttacgc ctctatttgt
     9241 aaagataatt ttataggtaa taatatcagt aaattatggc taaaagactt ttttataaaa
     9301 atatgatcga ttcgaaattt ataaaaatca caatttttaa ttaattgata taatttaatt
     9361 gttttataaa cgcaatttga gagtattagc tttattgtaa actgttaact aaaattttat
     9421 attgtatatg ctattggtta aaaattttgt atatttttga ggtgataaaa tgaattcttt
     9481 aagaaatggt gtttcttttc ttgtttgtat tgcaggtgct ttctttgcta ttgcgggtgt
     9541 agtatcgatg ataacacgtt ttatcggtta tgaggtaaaa atatttttat ggctttctga
     9601 atttgaaaca gttactgctt ggttaataag agcaggtgtt attgccggtg gtatacttgt
     9661 gtatgttttt ggagttttga tagatcctga acgataacta actagtttag gtcaaaatac
     9721 cttctttatt ccctttaatt atgtaaaatg tctttcgaat atgcttttta catacgaaac
     9781 ttttggagaa agtgcttatg tttggtaaaa taatagagtg gttttcacac agacaccttt
     9841 tggtaaatat tatcgcaata tctgttattt tgggagggat cattgcttgg gacaatacca
     9901 agaaggagga gatgccggat ataacttttc ctttttcacg tgttatggtc gtgtggccag
     9961 gggcatctcc agaagatatg gatcgctatg tcactgtgcc aatcgaaaaa gcacttagag
    10021 gtattgatgg aatcgaggaa atatccagta caacttctca ggggagtacc atattatctt
    10081 tatggctacc agccaaggcc tcagaaagtg agatggaaga tattgtacag gatataaagg
    10141 atgcaataga tagggttgac ctgcctgatg atatcaagga tgacccagaa gttagagtac
    10201 cgaatacaac gcggttttct gtaataaata tttccatgta cagagaatcg gccaaattat
    10261 ttagtaatga agacagaaaa aaagtgcagc agtatgcacg aaatcttgag aatagcttac
    10321 ttagccttac atctgttagt gaaattagct ggcgaaatta cagcgtaccc caggttaaca
    10381 tttaccttga tatcgcagct ttaaatgagc tcaatgtctc catatcagaa gcaatatctg
    10441 ccattcagaa tggaagtttg cgctctccgg taggaacgat gaagggagag tggagtcgat
    10501 ccgaagtaaa agaagataaa agacctgagg aagaaacgcg atcaataacc attgaaaaga
    10561 aaagctcaaa acaatctgaa tttaaagatt ataaaataat tatggacgca gagctgacaa
    10621 aggttgatga tcttgaaaat ttgattattc gtatgaactt tgagggtaaa gccgtttatt
    10681 taaaggatat tgcgcgtgta gtacccgaat ttgaggaaag cagtgcaatt ttaaaaacta
    10741 acggatatga agcagttacc ataaatgtta ccaaatcttc atctgcgggg attattgatg
    10801 cagttgatga aatcagaaaa aaagttgaag agtttaaaaa aaattcatta gcaggaactg
    10861 atataaacgt agttcttgtg gatgatgaat cgatagctgt taaagataga ttatccatgg
    10921 tgtcgggtaa tggaattctt ggatttgttt tagtagtaat atttttattt ttgtttttaa
    10981 atgcaaaatc tggagtatgg gttgccttcg gtctgccttt ctcgctttgt tttactctaa
    11041 ttggcatgta tatgcttggg tattcaataa acaatacaac attggccgca gtaattatcg
    11101 tcctcggaat ggtggttgat gatgccattg ttgttgcaga gaatataaac aggcttgagg
    11161 ccagtggaat gccaagaaaa caggctgtgg ttgagggaac aggaagggtt ttccttccga
    11221 tagttgcaag tattgtaaca acatgcgttg cgttcatacc tctgtttttc tttgggggca
    11281 gatttggagt tttaaatgca tttataccac ctgtaatttt tataatgctt tttgcttctc
    11341 tttttgagtc tgtttttata ttacctgcac atatgtcatc ttccttgttc gattctaaaa
    11401 aaggcgctac ggagcgaagt tggtttataa agttagaaga aggatatcaa caaattttgc
    11461 ggtttcttct cgctcatcgc aagccagtat tcgtttttgc aattattctt ttatttttcg
    11521 caggttttct atttactaca acaatgaagt atgaaatgtt tccgcgtgaa gaaagtactc
    11581 aattacgtat tgaaggtgag gttgcttatg gaacattaaa aaaatctact gctagaaaaa
    11641 ctgaggaagt tgaagccata attgatgaat atgtcggaaa tgaggctttg tcttatgaaa
    11701 caagaatagc acaaaccagg agaagtaatg ctgcccgtga aaatgtattt tcgataatga
    11761 tagagttagt gccacttgaa aaaagagatc gaaaatcgtc tgctattcaa aaagagatgg
    11821 aacagaaatt aaaaagatta aagggattcg aaaaacttga attttcacag ggccgttttg
    11881 gatctacaag tggaaatcct atagaagtac atgttcgcga taataatgat gaaacacgta
    11941 acaagctgac gcgagaactt ttacaggcca tgaagaacta cgctccatat attaatgtgg
    12001 acgaagaaag acagcttcag gatccggagt atctttttaa accaaaaaga acaatagcgg
    12061 gtagtttgaa tgtttcaatt agcagaatat tgagtgcttt aagaacagcg ataaacggaa
    12121 cgattttata tgaagtacca gaaaacggag atgatgttga ataccgtata actgttgaag
    12181 ataaggatag aagtaatctg gaattattaa ggaatttacc tgttttaaat aattcaaatt
    12241 attcaattcc tcttggaaat gtggtagatg ttatagaagg aaatagcgca aatacaatca
    12301 gtcgtatgga taatgtccga actactagaa ttttttcaga tattgatgcc tcgagtggtt
    12361 taacacccgt agagaccgct atctattttg aagaaaatat atttccagat ttattgtcgc
    12421 gttacccgac atcatctatt gtttatgctg gtgaggttaa agatacacgt caatcgacag
    12481 gagaatttat atattctgtt atagcagtgt tgctattgat ctatataatt cttgctcttc
    12541 tttttgattc tcttgttaaa ccttttatta ttatggctac aataccattt gccgcagtag
    12601 ggataatatt tgcgttaaaa ctacatggga tttctgttta tggtttcttt tcggtagtcg
    12661 gatgccttgg attgattggg gttgttgtaa atgattccat tgtaatgatt tctgttcttg
    12721 aagcaaatta caaggctcat ccgaatataa aaaaagcaat tgtagcaata tccgaaatag
    12781 ccaaaacaag gttgcgtgcg gtgatattaa caacaattac aactgttgca ggattgttgc
    12841 cgacggctta cggtattttg ggttatgatt caatgctctc agatatgatg ctcgttatgg
    12901 cgtgggggtt gatatttgga actattatta ctcttgtttt tgttcctata ctatatctta
    12961 tcatgtaccg aatacaacat cacgtcaagg atattgctaa ttccaattga tctggttttt
    13021 ttcaatgttt cggtgttttt gctacttagt tttgtattta atatgcaaaa agtaagaaat
    13081 aacaccactt attttgctat tttccgggtt tcttagccag aagaatagtc atgtgagtca
    13141 taaaagttca taccccagct attaataaag tatggaaaac caaagaagtt atgaacaaaa
    13201 acagcatgga aacaggggcg aataaactcc ttaacactgc tcataaactc gtcgaagaaa
    13261 ttcattgcca gaaacaacat gtacgataaa ctgtagcagt aaagattagc tacatctttt
    13321 aattttcttt tagaaatact tgaaaatctg atatgtactg atttgtagat ggtacctctc
    13381 tcgtgatatt tgttcagaga atcattacat actggattct tagccagcat ttttgagtac
    13441 ttatccagaa ctgtatttat aacatccgaa atatgcatat tatgcttatt ggcaaactct
    13501 tcaattttat caagtctgct aatttcaaca tcaattgatg ttctaaaatc tgtagtttcc
    13561 atttgccgcc tccttatgtc tgcatatata tactggaaaa aatgctgatt tgttcaattt
    13621 tttagaaaaa attctgcaaa aattttgggt aattatctga tactgtaaat ttctgtaaat
    13681 ataattcttt tcaaaaagaa ggatgcagtt aaaatagcct gtttgataat taatcattaa
    13741 aaaaatctgt attatattta agcgagaaca tattatgata aaaacagtat ttactaggtt
    13801 atatatacta ttccttttac ttgttatttt gaccatatct acaagctgtt ataaccaagc
    13861 taacatatca ataaatattg tcgaaaagca aaaacgtagg cttactgcag aactaacaat
    13921 aaacatgaaa aataatgaat ctttagatat aaatgatata aaaataaaat actttattga
    13981 taacgatttt attgaaatta aaaaaaattt ggctaaagat tctgtgaatg ttgattatct
    14041 aaatatagat aaaatagatg taagagaatt ttcagttact tcaaaaatga ctctggagaa
    14101 agacaaagcg ctgattaatt tcatgaaatt tacttcggat ctagataaaa ataataaata
    14161 taattgtcca ttaattct
//
LOCUS       DAOTGZ010000050        14095 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_3064
            NODE_5196_length_14095_cov_4.765741, whole genome shotgun sequence.
ACCESSION   DAOTGZ010000050 DAOTGZ010000000
VERSION     DAOTGZ010000050.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868088
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 14095)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 14095)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:22:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,785
            CDSs (total)                      :: 2,751
            Genes (coding)                    :: 2,732
            CDSs (with protein)               :: 2,732
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 12 of 19
            Pseudo Genes (internal stop)      :: 5 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14095
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5196_length_14095_cov_4.765741"
                     /isolate="AMR_MDS_3064"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            377..>2021
                     /locus_tag="P5123_05010"
     CDS             377..>2021
                     /locus_tag="P5123_05010"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRX15650.1"
                     /translation="MVKISKSANQQISKSALITFFLMVLLVFTAFGCDVEEPKFKREV
                     KAENGRFAIRERVIKKDAKEIKNILSLVDERKRSSRNMRGIRSAEDEMSDEDFEDQRI
                     LSIYEPQDVVDGLVEIIHDLPEEDLAQLEDAEEIEFTLSEDIDLDEIDARFISRKAED
                     EIVVTDPAIIGDRIESDICNLVIDTLTERYMPTALLAQNYPELFSVEIEENMLDGEVD
                     LFKRITSGILSEGEVKKYETIIRDYTQNFNFTRLEELFVELEENEKLKEQILVEDGFT
                     DADEDEDEDDGDTEPVDIDEDLLAQIEDEEMNAARAAKLTRKQKNKIIGTRLAKNYPG
                     AIMLNHRSDSFVSYQRAVPALKAGIFGVLFGKKQSQTHGGMFSKQIWDRLLKREGGTP
                     KAIMSAGANSKTEWIHGMATKLDGMNNYERKSKKGRILLPLKYKPSDGVAAEKAAYNS
                     FKFSDEGKGTIAYWIPVLDYFRVTNRTKSKSDKSFAYCTRIPYYGWKWSNKRRVNLDS
                     RDDFFAGNLVTPHNVKRATRDRFFKLPYLSVKRKGWRIRI"
     assembly_gap    2022..2121
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <2122..2474
                     /locus_tag="P5123_05015"
     CDS             <2122..2474
                     /locus_tag="P5123_05015"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRX15651.1"
                     /translation="ERIKYYKIIDFENKELELIYEFVGQGDYVSYGVVLGDYAYLAND
                     YDFSYQRFYKVDMTTKNIVSQISMVSSSSLIHKNGYFWLSDDWSYPDDGNIYYYRINP
                     DDLSVDKYKIDEGK"
     gene            2707..3804
                     /locus_tag="P5123_05020"
     CDS             2707..3804
                     /locus_tag="P5123_05020"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRX15652.1"
                     /translation="MITRIKKLILILSVLLTWVVINSCGLLNDIDIKRDYPKQFDSIE
                     YSDDVLVLQTTFNKLVAIDTLTAKQLAVYDFTTKDHELGKGVHYKKHYYVTMENIEDS
                     QYALLLRLNIETGKVDEIKYYNDFPELDSHARNLLLVGSPCGGHDTMLYDMETGKSEK
                     KKTYASFGKTPIFFRNNLYVIDNTQPGDDELSWVSYTNGTRFTYDDTEYIGGFKRVGS
                     ENYIMMIQSNKRNQELEDNTIAIVGNDMSTGNKRIKYYKIINFENRELELIYDFINQG
                     SVVALGVIVDDYAYLANDFGFSKTVLFKVDMATKEIVAEAILPHNSTLIYKNGFYWCS
                     EFDISGDEGDNYYRVNPDDMSVVKFKIDESK"
     gene            3801..4715
                     /locus_tag="P5123_05025"
     CDS             3801..4715
                     /locus_tag="P5123_05025"
                     /EC_number="3.4.23.49"
                     /inference="COORDINATES: protein motif:HMM:NF013446.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_function: GO:0004175 - endopeptidase activity [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="omptin family outer membrane protease"
                     /protein_id="HRX15653.1"
                     /translation="MRKVLILILLLALIDNNLLAEEDGATVKKGNLSFLITTGTGFAQ
                     TNVNEYVYDKSRTVSQLDWENNTFLVHCFCINASWKNMVITGYAKNSFKSLDGNVENS
                     DWVGTTSDKTHYSRHDSIVNRYANYGFIAGYNLLFGSFILTPGIGYGYTGVSLHANDG
                     YYKYPPIAPKEEVYGTAIIYTLKYHFPFASLGLAFVYNKLRLDLNLRYSCVGTLDAKD
                     SHVMRSIDFYDSFKSLKYYLVEPKISYFATKKLSFFISCHSEYVPKKRGSSYFIDTES
                     GDKSYKEKDVVGNSYKSLMIIAGVGFKI"
     gene            complement(4891..6192)
                     /locus_tag="P5123_05030"
     CDS             complement(4891..6192)
                     /locus_tag="P5123_05030"
                     /EC_number="1.2.1.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014625604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004350 - glutamate-5-semialdehyde
                     dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0016620 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, NAD or NADP as
                     acceptor [Evidence IEA];
                     GO_process: GO:0006561 - proline biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate-5-semialdehyde dehydrogenase"
                     /protein_id="HRX15654.1"
                     /translation="MTLHETAQHSKKASIYLASKDENSKNSALETIAQQLDSRREEIK
                     AANSTDIQRSKSENLAQPLLKRLLFDDNKIDQTIAGIKDLVTLKDPVGKTLLSTELDT
                     GLELYKISCPIGVIGVIFESRPDALVQIATLCLKSGNALLLKGGSEAMETNRILADII
                     YNASLEASMPEGWLYLLETRSEVKEMLSLDDSIDLIIPRGSNEFVKYIMDNSNIPVLG
                     HADGICHLYIDKDADPEMAVNLTIDSKVQSVAVCNALETLLVHEDIAEIFLPAVRKRL
                     DESGVEITGCDKTARIIECEAADDDTWKNEYLDYKLSIKVVSSLEEAVDHINCYGSGH
                     TDSIVTNNGHAAGIFMDLVDSGNVFWNASTRFSDGFVYGFGAEVGISTNKIHARGPVG
                     LEGLLIYKYKLIGRGHVRADYTGGSKRYTHNPINKPYPHND"
     gene            6724..7047
                     /locus_tag="P5123_05035"
     CDS             6724..7047
                     /locus_tag="P5123_05035"
                     /inference="COORDINATES: protein motif:HMM:TIGR00377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043856 - anti-sigma factor antagonist
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="STAS domain-containing protein"
                     /protein_id="HRX15655.1"
                     /translation="MLNTRVENSYVIVEMPENIDLYNAAEIKKTLFPLSEENDFIAID
                     FEKVKVIDSSGLGVLAVLSKQMALESKSFALFNIPNNIFQIMKITSADKYFSIYNSFD
                     EIQRS"
     gene            complement(7044..9119)
                     /locus_tag="P5123_05040"
     CDS             complement(7044..9119)
                     /locus_tag="P5123_05040"
                     /inference="COORDINATES: protein motif:HMM:NF018884.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016791 - phosphatase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SpoIIE family protein phosphatase"
                     /protein_id="HRX15656.1"
                     /translation="MKKSILILLFIFFSCGSYDSAIFDIASGSINLSESNLESGNIIS
                     HITYVKDRYIRELPKESSIKTISFTPKELKSSTIEQLEPGTYIFTLSIPESAGITVFR
                     SPFFNTSCNVFVDRRLLGSFTERNIHHPQKRAVTIPSAGTVTVAIHLYGKLGENDFES
                     FSLESLTLTQQKSIRYILLSVMIILLLLANSFSHIKNLKIQKREIQLLFSAIAPILAS
                     LQIALVTGFFMEISIPNTIFLRLYYPLGALSIICLIISLYHIHLSAIDKKLVIISSVT
                     FFFITLLLIIPPIVSLAYIIPAIYTILLVLFIIMAVTVVKTDVFENRFQTLLFIISVI
                     LLTLGVITDAFSPLYLYHSRYVYQATLLLFMIILISPAHKKLTGCFASEQKLTQELAI
                     KSAKLVKANFELKVLNQNLEETVNIRTNEYKNALSSVEATLRELEKAHASIEHDLVMA
                     SNVQRLYFSEKIPRVKGWEIAFIYNPMHQVSGDMYDFYVSNNTLLGATLFDVSGHGVQ
                     SALITMLAKNLISELYYKLYEKPAGTILEIINKRLIEEIGSIENYMTGVLMKIKENSI
                     EVANASHANILHRSHSSNTVRELAPKMSKGNFLGIGIMDEPYGTDTYLIEEDDSLLLY
                     SDGLYEYRHDGVLLTREFDLIKENLMKCKKYQSAQSQLDQILKSLSISEKKTLGDDLT
                     VILLKKVQD"
     gene            complement(9384..9917)
                     /locus_tag="P5123_05045"
     CDS             complement(9384..9917)
                     /locus_tag="P5123_05045"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015229650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HRX15657.1"
                     /translation="MNPSSRLTKEQEKERYLQHNNDVNDPRYQKFVKPVVDHILEFFT
                     KEKKGLDFGCGTGPVISKMLSDSGYTTSLYDPFFYPGNSALKDTYDYIICCEVAEHFT
                     NPSAEFALLHSLLNTDGQLICMTELFTDTVDFEKWHYNNDPTHYIFYQKETLAYIQKA
                     VGFKSFKTDGRLIVFYK"
     gene            complement(10048..11646)
                     /locus_tag="P5123_05050"
     CDS             complement(10048..11646)
                     /locus_tag="P5123_05050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011433470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 3"
                     /protein_id="HRX15658.1"
                     /translation="MSNPEITKEINRRRTFAIISHPDAGKTTLTEKLLLYGGALELAG
                     SVSSKKQSATSSDWMELERKRGISISSTVLQFEYNGYKLNLLDTPGHRDFSEDTYRVL
                     MAVDFVIMVIDAGKGIESQTLKLFEICRKRDVPIFTFINKLDRPSRPPLDLIDELEKV
                     LNIHAYPANWPLDNGPDFKGVYDRLKKEFNLFERVPGGSYRVPVSLHDASDPFIKEML
                     HAETYKEINEELEMLDIAQEGFNEEAVLAGHTTPVFFGSAVNNFGVELLLKNLIEYSC
                     PPMPRYIDGEAFPMESPVFSGFVFKIQTNMNPQHRDRMVFARICSGVFHRDMSVFNTR
                     SRKEVRLGSSHNIFGRDREIVDTAYPGDIIGFITNADFTIGDTISSDASISFNEIPRF
                     APECFAYLLNTTMSAAKSFRKGLDHLLAEHIVQSFFLNNNFGQPLLGAVGPLQFEIMQ
                     YRLKDEYGVETRLENKPWQVMRWLPCGITGAEIESSLPYNSTFGCDDQKREVLFFQNE
                     WGLKSFIKDNPEIELFDTPPVKED"
     gene            complement(11787..13202)
                     /locus_tag="P5123_05055"
     CDS             complement(11787..13202)
                     /locus_tag="P5123_05055"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRX15659.1"
                     /translation="MDLKQFQFTEHLIERFKNENHIPVNFYNRFGQILIHKKENASVH
                     EVNQLLKFQGTGIYYDQNDYDTLTGKNRISPSSAGSKQCIPKGLTDTKLFSQQTADRL
                     AGFTSELFDRLKKESIDSVTARNAAALLSDIFTDFARQPDAMTGLLNILELMNASNDE
                     YEVILAVKRTVISMALKTRGMLAVSNDERKRLNDEITTVMIASLLSDIAFLKMDLPRD
                     RKLTPQEYVKMKRHPLISYLMIANEHALNHNAKHLVLFQHYRQKDKPNNYPDMFLIQS
                     ELKKRLSKISDTALQLHALKTGAIYSHNANITGIASKFSYLTTPTPYKKSYSAIDAVR
                     AIINNATFTYSNQAIREFLDYSAISLCDNNEIIQSGDIIITEASSFDGGKNYEICMVM
                     ESSRFQSRPVIKRFATLSPHIKTRPKTAFADFNLLSIEPDKRHADIDLNRDNSRFIAY
                     VIDPEYDPALFKAATSLISQT"
     gene            complement(13260..14033)
                     /locus_tag="P5123_05060"
     CDS             complement(13260..14033)
                     /locus_tag="P5123_05060"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRX15660.1"
                     /translation="MKKLLSIFIFFCVTANAETMFLAGPYGGFSSNTQNLENNIKLEE
                     TASVFGLYTQVINPELFQINNFLYHSPDINDTKITGNHFIADFYPYSFTIGSLAVGAG
                     MNYLSTDTSRDDFKMDQSVVTPYARLGQNVIFGESTNLRIFPWIGFGKKYSESEGSFT
                     FSPAEGAPSTTVPLESDTDENVFLTGINLHFTFDRYFCVQFKYLIDRNLKTETNNYSL
                     SAILNAYVHKHAGISYRFMYNEYTTGEDIINMVGIFYKY"
BASE COUNT         4197 a         2458 c         3005 g         4335 t
ORIGIN      
        1 tgctgctcgc atatgcagcc tatgctggtc gcaagatgac tcttgctgct tgcatatgca
       61 acctatgctg aaaagttaat tattaaagat tacccgataa tagtgaatta aattaagttg
      121 tttgtcaact aaatagcagg gctgagagat acagggatta attgagtagg tgtttttgta
      181 atcttattat aataataagt tacgctgatt gagatttttt cctaaatata aacatattta
      241 tgattacaca aatatgtact acataatatt aaatatttct tttagttgtt tttgtatatg
      301 tggataataa agtagttgac tcttgtatat tttaaaggta tttgaatata ctaaaaaata
      361 taaaaggagg tgcgttgtgg tgaaaatcag caaatcagca aatcagcaaa tcagcaaatc
      421 agcactgatt acgttttttt taatggtgct tcttgttttt acggcctttg gatgtgatgt
      481 cgaagagcca aaatttaaac gagaggtaaa agccgaaaac ggacgttttg ccatccgtga
      541 acgtgtgatt aaaaaagacg cgaaagagat taaaaacatt ttgtcacttg tggacgagag
      601 aaaacgatca tcacgtaata tgcggggaat cagaagtgcc gaagacgaga tgagtgacga
      661 ggattttgaa gatcagcgta tcttgtcaat ttatgaacct caagatgtcg tagacggtct
      721 tgtagagata atacatgatc tgcctgaaga ggacttggcg cagctagaag acgccgaaga
      781 gatcgaattt acactttctg aggatattga tctcgatgag atagatgcaa ggtttatctc
      841 gcgaaaagcg gaagatgaaa ttgttgttac tgatcctgca attattggcg atcgtatcga
      901 gtcggatatc tgtaatctgg ttatcgatac actgaccgaa agatatatgc caaccgccct
      961 gcttgctcaa aactatcccg aacttttttc agtagaaatt gaagagaata tgcttgatgg
     1021 agaggtagac ctttttaaaa ggattacgtc cgggatactt agtgaaggtg aagtaaaaaa
     1081 atatgaaaca atcatcagag attataccca aaactttaac ttcacaaggc tcgaagagct
     1141 gttcgtggaa ctagaagaaa acgaaaagct gaaagagcag atacttgtcg aagatggatt
     1201 tacagatgcc gatgaagacg aagatgaaga tgatggcgac actgaaccag tagacattga
     1261 tgaggatctt ttggctcaga tcgaagatga agaaatgaac gctgcacgag ctgctaaact
     1321 tacacgtaaa caaaaaaaca aaataattgg aacaagactt gccaagaact atccgggagc
     1381 aattatgctt aatcacagat ccgattcctt tgttagctac caacgggcag tacctgcttt
     1441 gaaagccggc atttttggtg tgctgtttgg caagaagcag agccagacac atggcggcat
     1501 gtttagtaag cagatttggg atcgtttgct taaaagagaa ggtggaacac caaaagccat
     1561 tatgagcgcg ggggcaaaca gcaaaacaga atggatacat ggtatggcca caaaactgga
     1621 tggaatgaat aattatgaaa gaaagtcaaa aaaaggaaga attttattgc ctttgaagta
     1681 caaaccctct gatggtgtag ccgccgaaaa ggccgcctat aacagtttta aatttagtga
     1741 tgaaggcaaa ggaactatcg cttactggat acctgttctt gactatttca gggtaacaaa
     1801 cagaacaaag tctaagtcag ataaatcttt tgcttattgc acgcgtatac catattatgg
     1861 gtggaaatgg tcaaataagc gcagggtcaa tctggattca agagacgatt tttttgccgg
     1921 gaatcttgta actcctcata atgtaaaacg cgcaacaagg gatcggtttt ttaaattacc
     1981 gtatctctcg gtaaaacgaa aaggctggag aatacgaatt annnnnnnnn nnnnnnnnnn
     2041 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2101 nnnnnnnnnn nnnnnnnnnn ngtgaaagaa taaagtatta taaaataatt gattttgaaa
     2161 acaaagagct tgagctaata tatgaatttg ttggacaagg agattatgtg tcttatggtg
     2221 ttgttttggg tgattacgcc tatttagcta atgattatga tttttcttac caaagattct
     2281 ataaagtcga tatgactacc aagaatatag tgtcacaaat atcaatggtt agttcgtctt
     2341 ccttgataca taaaaacggt tatttctggt tgtctgatga ctggagctat cctgatgatg
     2401 gtaacatcta ttattaccgg ataaaccctg atgatcttag tgtggataaa tacaagatag
     2461 acgagggcaa atgaattgca ttatcttgta accccacata atgtaaaacg cgcgataagg
     2521 gatcgatttt ttaaattacc gtatctttcg gtaaaactaa agggtttgaa aatacgagta
     2581 aaaacgaagt ggaagaaagt atatacgtac agggctgttg cacaagagtt acgattaacg
     2641 gattatgaat attaataaac gcggccgtaa ggccgcttta cttaaaataa taaccgggtg
     2701 ataataatga taaccagaat caaaaaacta attttgatat tatctgtttt gcttacatgg
     2761 gtcgtgataa acagctgtgg tctattaaat gatatagata taaaaaggga ttatcccaaa
     2821 cagtttgact ctatagaata tagtgatgat gttcttgttt tacagactac ctttaacaag
     2881 ttggttgcta ttgatacatt aaccgcgaag cagcttgctg tatacgactt tacgacaaaa
     2941 gatcatgagc ttggcaaggg tgtacattat aaaaaacact attacgtaac aatggaaaat
     3001 attgaggatt ctcaatacgc gttactgtta aggctaaata tagagactgg aaaggttgac
     3061 gaaattaagt attataatga ttttcctgag cttgattcac acgcaaggaa tcttttattg
     3121 gttggcagtc cttgtggtgg tcacgatacg atgctctatg atatggagac cggaaaatcc
     3181 gaaaaaaaga agacatatgc ctcttttggt aaaactccga tattcttcag gaataatctg
     3241 tatgtaattg ataatacaca gcctggagac gatgagttga gttgggttag ttatacaaac
     3301 ggaaccaggt ttacatatga tgatacagag tatattggag gtttcaaaag agttggatct
     3361 gaaaattata taatgatgat acagtctaat aaacggaacc aggagcttga agataataca
     3421 atagcaatcg taggtaacga tatgagtacc gggaataaaa gaataaagta ttacaaaatt
     3481 atcaattttg aaaacagaga gcttgagctt atttatgatt ttataaatca gggttcagta
     3541 gtcgcgttag gtgtaattgt cgatgactac gcctatcttg caaatgattt tggattttca
     3601 aaaactgtgc tttttaaagt agatatggca actaaagaga ttgtcgccga agctattttg
     3661 ccgcataata gtaccttaat ttataaaaac ggtttttatt ggtgttcgga atttgatatc
     3721 tcaggtgatg agggtgataa ctattatcgt gtcaaccctg atgacatgag cgtagtaaaa
     3781 ttcaaaatag atgaaagcaa atgagaaaag tcctgatatt gattttattg cttgctctga
     3841 ttgataataa tcttttggca gaggaagatg gtgcgacggt aaaaaaagga aatttgtctt
     3901 ttttgataac aactggtacc ggttttgctc aaacgaatgt taacgagtat gtatatgaca
     3961 aatcccgaac ggttagtcag cttgactggg aaaacaatac ttttttagtg cattgttttt
     4021 gcattaatgc aagctggaaa aacatggtta taaccggata tgctaagaat tcatttaaat
     4081 cgctcgacgg caatgtcgaa aactcggatt gggttggtac aacttcggat aaaacacact
     4141 attccagaca tgattctatt gtcaataggt atgccaatta cgggttcatt gcgggttaca
     4201 accttttatt tggtagcttt attctgacac ccggtattgg ctatggatat acgggtgtca
     4261 gtttgcacgc aaatgacggt tattacaagt atcctccaat agcaccaaag gaggaagttt
     4321 atggaacggc aattatttat acattaaaat atcattttcc gtttgcaagt ttggggcttg
     4381 cttttgttta taataagcta aggctggatc tgaatttacg gtacagttgt gtcggaactc
     4441 ttgatgctaa agattcacat gtaatgcggt ctatcgattt ttatgacagt tttaaaagct
     4501 tgaaatacta cctcgtcgaa ccgaaaatat catatttcgc aacaaaaaag ctttcctttt
     4561 ttataagctg ccattcggaa tatgtcccaa aaaagagggg ctcatcgtat tttatcgata
     4621 ccgaaagcgg agacaaatca tataaagaaa aagatgttgt tggtaacagc tataagagct
     4681 taatgattat tgctggagtc ggatttaaga tataatcaag aaagaacaaa tttgtcagga
     4741 tttgagcagt tcgcctggat gctggttata atataattca tttaaattat tatgttgata
     4801 agtgaaaatg gcttgtccgg tttgttggtt cacctgtaaa caaattcaat ccgctgccgg
     4861 ttacgtttta accggcagcg aagtatttgt ttagtcatta tgggggtacg gtttgtttat
     4921 tggattgtga gtatatctct ttgatccgcc agtgtaatcg gcacgtacgt gaccccggcc
     4981 tatcagttta tatttgtata tcagaagacc ttcgagacct accgggccgc gtgcgtgaat
     5041 cttgtttgtg cttattccga cttccgcgcc aaagccgtac acaaatccgt ccgaaaaccg
     5101 ggtcgaggcg ttccagaaga cattacctga gtcgacaaga tccatgaaaa tgccggctgc
     5161 atgcccgtta tttgtaacaa tagaatctgt gtgtcctgat ccatagcagt taatatggtc
     5221 aacagcttct tccagagaag acacaacttt tattgagagt ttatagtcca gatattcgtt
     5281 tttccaggtg tcgtcatcag cagcttcgca ctcgattatg cgtgcggttt tatcacatcc
     5341 tgtaatctca acaccagatt cgtccaagcg tttcctgact gcgggcagga agatttcggc
     5401 aatatcctca tgtacaagaa gggtctctaa cgcgttacat acagctaccg actgaacttt
     5461 tgagtcaatc gtaagattta ccgccatttc ggggtcagca tccttgtcaa tataaagatg
     5521 acagattcca tcagcatgac ccagaacagg aatattagag ttatccatta tatattttac
     5581 aaactcgtta gaaccacggg gaattatcag gtcaatgctg tcgtctaaag agagcatctc
     5641 tttaacttcg gatcgggttt caagaagata cagccagcct tcgggcatag aggcctcaag
     5701 tgaagcatta tagatgatat ctgccaaaat acggtttgtc tccatggcct cgcttccgcc
     5761 tttaagaaga agagcgttgc cgcttttaag gcaaagtgtg gcgatctgta ccagagcatc
     5821 gggacgggat tcaaaaatta cacctattac gcctattggg cagctgatct tgtaaagttc
     5881 aagccctgtg tcaagttcgg ttgagagaag ggttttgcca accggatctt taagggtaac
     5941 aaggtctttt ataccggcaa ttgtctggtc aattttatta tcatcaaaga gcagccgttt
     6001 taacagtggt tgcgccaggt tttctgattt gcttcgctgg atatctgtac tgttagccgc
     6061 cttgatctct tcccggcggg aatcaagctg ctgagcgatt gtctcaaggg cgctgttttt
     6121 cgaattttca tctttcgaag caagatatat agatgctttt tttgaatgct gtgccgtttc
     6181 atgaagtgtc atgaacgtac ctcccaaaat gataatacca ttcgactgtt tagaaggccg
     6241 ttttgtcaat ttattttgca atacgctatg cccggagaat ttagaataat attattttca
     6301 atttttatta aaaaaatcta tttttcatac tttttttggc attaattttg cttttatttt
     6361 gttgtatagg gtgtaatgag ctattaaata gtatgcttaa ctaactattc cgatcattgc
     6421 tataaaaggc tgtgaagctg taacaccgat cataattaca gttatcaaaa cagaacacgg
     6481 ctttttatag aaacttcctc cttaatttag aagtgaccac cttgttactg cccctccccc
     6541 ggggcagttt tttttgccgt tttgaatata ttgtttcttc agcgggctgc tttatgtcaa
     6601 aaaacaggtg caattcccgg tttttgtatg ctccggagca cctgtggaaa atttcattaa
     6661 gcaattttgc tgcaaaaccg ttttttgctt tacattggca atataatttt atatataaga
     6721 tttatgttaa acacaagagt agaaaattca tatgtaattg tagagatgcc tgaaaatatt
     6781 gatctttaca atgcagcgga aatcaaaaaa acgcttttcc ccttatcgga agagaacgat
     6841 tttattgcca ttgactttga aaaggtaaag gtgatagatt cttccggtct tggtgtgctg
     6901 gcagttttaa gcaaacagat ggctcttgaa agtaaatctt tcgctctttt taatattccg
     6961 aacaatattt ttcaaatcat gaagattaca tcagcagata agtacttctc catttacaac
     7021 tcgtttgatg agattcaacg ctcttagtcc tgtacttttt tcagtaatat tactgtcagg
     7081 tcatccccga gtgtcttttt ttcgcttatt gagagcgact ttaatatttg atcaagctga
     7141 gactgtgccg actgatattt tttacatttc ataagattct ctttaattaa atcaaattct
     7201 cttgtcaata atactccgtc atgacggtat tcgtaaagcc cgtcgctgta aagcaacaga
     7261 gaatcatctt cttcaatcag ataagtgtct gttccgtatg gttcatccat tatgccgata
     7321 cctaaaaagt ttcctttgct catttttggt gcaagttccc tgacagtgtt gctggagtgc
     7381 gatctgtgca gaatatttgc atgcgacgcg tttgccactt ctatggagtt ctcttttatt
     7441 ttcataagta cgccggtcat atagttttcg atgctcccta tttcttctat aagccgtttg
     7501 tttataattt ccagtattgt tcctgcaggc ttttcataca gtttgtagta aagctcagag
     7561 ataagattct tggcaagcat tgttatcagg gctgactgca caccgtgccc cgaaacatca
     7621 aaaagagtgg cacctaataa ggtattattg ctgacataaa aatcgtacat gtctcctgaa
     7681 acctgatgca tcgggttata tataaaagcg atttcccatc cttttacacg gggtattttt
     7741 tcgctgaaat agagtctttg tacattggat gccataacca gatcgtgctc gatagacgcg
     7801 tgagcttttt caagttcccg cagtgttgct tctacactgc ttaaggcgtt tttatactcg
     7861 ttagtacgga tgtttacggt ctcttcaagg ttctggttta atactttaag ttcaaagttt
     7921 gcttttacaa gcttggccga ttttattgcc agttcctggg taagtttttg ttcagacgcg
     7981 aaacagccag taagtttctt atgagcgggg ctgattagta ttatcataaa cagaagaagt
     8041 gttgcctgat aaacataacg ggaatggtac agataaagtg gagaaaatgc gtccgtaatt
     8101 actcctaaag tcagtagaat aactgatata atgaaaagca gtgtttgaaa tctgttttca
     8161 aagacatcag ttttaaccac agtaactgcc ataatgataa acagaacaag tagaatcgtg
     8221 taaattgccg ggataatgta tgccagagac acaatcggcg gtataataag aagaagagtt
     8281 ataaagaaaa aggtgactga agagataata acaagcttct tgtctattgc agacaaatga
     8341 atatggtaca gtgaaattat aagacagata attgaaagag ccccaagcgg ataatagagc
     8401 cggagaaata tggtgttcgg aatggagatt tccataaaaa aaccggttac cagggctatc
     8461 tgtaaagatg caagaattgg cgctattgcg gaaaacagaa gttgaatctc gcgtttttga
     8521 attttcagat tttttatatg tgaaaatgaa tttgcaagta acagcagaat tatcattacg
     8581 gacaatagta tataacggat tgatttttgc tgtgttagag ttagtgattc cagggaaaaa
     8641 gattcgaaat cattttcacc aagttttccg tacaggtgta ttgcgactgt aactgttccg
     8701 gcagaaggga ttgttaccgc tcttttttgc ggatggtgaa tattgcgttc tgtaaaagaa
     8761 ccgagcagcc ttctgtctac aaaaacatta cagcttgtgt taaaaaatgg tgatcgaaaa
     8821 acagtgattc cagcggattc gggaattgag agtgtgaata tatacgttcc gggttcaagt
     8881 tgttcaattg tgctgctttt tagttcttta ggagtaaatg atatcgtttt tatggaactt
     8941 tctttgggta attctctaat gtaacggtct tttacgtacg taatatgcga tattatattt
     9001 cctgattcaa gattagattc ggagagatta atgctgccgc ttgcgatatc aaaaatagct
     9061 gaatcgtagc tgccgcaaga aaaaaagatg aataaaagta tcaatattga ttttttcaca
     9121 aattatcctg aaaactgttt aaaacagatt ataaaagata aaaaaaataa tgcaattgtt
     9181 ttttaaattc tttgtaatct tgcacttgat tggaagagat aacgtaatcg tataacaacg
     9241 aggcagtact tatgattaca ctagcctcag ttgctgcact ttttatttta atgaagctga
     9301 ctgctgctgt tgactgggaa aacaagtggt aatgcagtat tattataaag cacaggctgg
     9361 ttcttagcaa ccgtttttgc cggctactta taaaaaacaa taagccgccc gtcggttttg
     9421 aaagatttaa atccgacggc tttctgaatg tatgcgagtg tctctttttg atagaagata
     9481 tagtgagtag ggtcgttgtt gtagtgccat ttctcaaagt ccactgtgtc tgtaaaaagc
     9541 tcggtcatgc aaataagctg gccatcagta tttaaaagag agtgcagaag tgcaaattcg
     9601 gctgacgggt ttgtgaagtg ttccgcgact tcacagcaga ttatgtaatc gtatgtatct
     9661 tttaacgcac tgtttccggg gtaaaaaaaa ggatcgtaaa gtgatgtcgt gtagccggaa
     9721 tcagacagca ttttggatat aacaggcccg gttccgcagc cgaaatccag accttttttc
     9781 tcttttgtga aaaattcaag tatatggtcg accaccggtt taacaaattt ttgatatctt
     9841 gggtcattta catcgttgtt gtgctgaaga taccgctctt tttcctgctc ttttgtcagt
     9901 cttgaggacg gattcataaa gagactccgg cagtccgggc ataaaaaata gtcaatcttt
     9961 ctgttgcttt ggtaatagtc cgatatgctt ccgcatattg cgcatggtga atattttgcc
    10021 gggtctgaca ttttatgatt atcctcttta atcttctttt accgggggtg tatcgaaaag
    10081 ttctatttca gggttatctt ttataaatga ttttaatccc cactcgtttt gaaaaaaaag
    10141 aacttcccgt ttttgatcgt cacagccgaa tgtcgaattg tatggtaaag agctttcaat
    10201 ctctgctccg gtaataccgc aaggaagcca gcgcattacc tgccatggtt tgttttccag
    10261 tcgggtttcg actccgtact catcttttaa ccggtactgc attatttcaa actgaagagg
    10321 accaacagcc ccgagcagag gctgcccgaa attattgttc aaaaagaaag attgaacgat
    10381 gtgctcggca agaaggtgat cgagcccttt tcgaaaggat ttagccgccg acatggttgt
    10441 atttaaaaga taggcaaagc actcaggagc aaatcttggg atttcgttaa aggatattga
    10501 ggcatcagaa cttattgtat cgccaattgt aaaatcggca tttgttataa aaccaataat
    10561 atcacccgga taggctgtat ccacaatctc acggtctctc ccgaaaatat tgtgtgagct
    10621 tcccagccgt acctctttcc ggcttcgagt attgaaaact gacatgtcgc ggtgaaaaac
    10681 acccgagcat atccgtgcaa aaaccattct gtcccggtgc tgcgggttca tatttgtctg
    10741 tatcttaaaa acaaaaccgg aaaagaccgg actttccata gggaatgctt caccgtctat
    10801 atagcggggc attggagggc atgaatattc aatcagattt ttaagtaaca gttcgacacc
    10861 gaagttgtta accgcgctcc caaaaaaaac gggagtagtg tgtcctgcaa gaacagcttc
    10921 ttcattgaaa ccttcctgcg ctatatcaag catttcaagt tcttcgttaa tctctttgta
    10981 ggtttccgcg tgaagcatct ctttaataaa ggggtcagaa gcatcgtgaa gagaaactgg
    11041 tacccggtac gaacctccgg ggacacgttc aaaaagatta aactcttttt ttaaacggtc
    11101 gtaaacacct ttaaagtcgg ggccgttgtc taatggccag ttagccgggt aagcatgaat
    11161 attaagcact ttttcaagtt catcaataag atcaagaggc ggtcgcgagg gacggtccag
    11221 tttgttgatg aaagtaaaaa taggaacatc ccgtttgcgg catatttcaa acaatttaag
    11281 ggtttgactc tcgataccct ttccggcatc aataaccata atgacaaaat caacggccat
    11341 cagcacgcgg tacgtgtctt ccgagaagtc cctgtggccg ggagtatcca gcagattaag
    11401 tttatagccg ttatactcga attgcagaac agtcgaagat atcgaaatgc cgcgtttacg
    11461 ttcgagttcc atccagtcgg atgatgtcgc gctctgtttt ttggagctta cggatccggc
    11521 aagctcaaga gctcctccgt aaagaagaag tttctctgtc agtgttgttt tgcccgcatc
    11581 agggtgtgaa attattgcga aggttcgccg gcggtttatc tcttttgtaa tttcgggatt
    11641 actcataaat tctctcgtct ataatgttat taagttaact ggttaaatgc tgccgccatt
    11701 attttgtaca atccggcact gtcaatacta tatcagcgct agaatatggt gataacttgc
    11761 cgggcttggc ttcaaagtgt atcgggtcag gtttggctaa ttaaagatgt ggcggcctta
    11821 aaaagcgccg gatcgtattc agggtcgatt acataagcta tgaatctgga attgtcgcgg
    11881 ttaagatcaa tgtcggcatg acgtttgtcc ggttctattg agagcaggtt aaaatcagcg
    11941 aaagcagttt tgggtctggt ttttatgtgc ggcgagagtg tggcaaatct ctttattacg
    12001 gggcgacttt gaaatcggga actctccatc accatgcaga tttcgtagtt cttgcccccg
    12061 tcaaaagaag aggcttcggt aataataata tcccctgact gtataatctc attattatca
    12121 caaagactta ttgccgaata gtcaagaaac tcgcgtattg cctgatttga ataggtgaat
    12181 gtggcgttgt taataatagc gcgtactgcg tctattgccg agtagctttt tttgtatggt
    12241 gtcggagtgg tcagataaga aaactttgat gctattccgg taatattggc attatgcgaa
    12301 tagatagccc ccgttttaag cgcgtgaagc tgcaaggcgg tatcgctgat ttttgaaagt
    12361 ctttttttta actcgctctg tattaaaaac atgtcggggt agttgttagg cttgtctttc
    12421 tgacggtagt gctggaaaag taccagatgt ttagcgttat gattcagggc gtgttcgttt
    12481 gctatcataa gataagaaat aagcggatgg cgcttcattt tgacatattc ctgaggtgtt
    12541 aactttctgt ctcggggaag atccatttta agaaaagcga tatcagacag cagtgaagct
    12601 atcataactg ttgtgatctc gtcattgagt cttttccgct cgtcgttgct gacagcaagc
    12661 ataccccgtg ttttaagcgc catagagatt acagtacgct taactgctag tattacctca
    12721 tactcatcat tacttgcgtt cataagctca agaatattta atagtcctgt catggcatca
    12781 ggctgtctgg caaagtctgt gaaaatatcc gacagcaacg cggcggcatt tcttgcggtt
    12841 acagaatcga tgctctcttt ttttaatcga tcaaaaagtt cagatgtgaa tcctgccagt
    12901 ctgtctgctg tttgttgcga aaaaagtttt gtatcggtta atccctttgg tatacattgt
    12961 tttgagccgg cggacgaagg ggaaattctg tttttaccag tcagggtatc gtaatcattt
    13021 tgatcataat agattccggt accctggaat tttaaaagct ggttaacctc atgaactgac
    13081 gcgttctctt ttttatgaat aagtatctga ccgaaccggt tgtaaaagtt aacaggaata
    13141 tggttctcgt ttttaaaacg ttcaataagg tgttctgtaa attgaaactg tttaagatcc
    13201 atactccttc tccggttttt atttttaacc cgaaagaaac tatacagtac tgactgctgt
    13261 cagtacttgt aaaatatacc aaccatattt ataatatctt ctccggtagt atattcgttg
    13321 tacataaagc ggtaagagat accggcgtgt ttatgtacat aagcattaag tattgccgat
    13381 agtgaatagt tatttgtttc ggtcttaaga tttctatcaa taaggtattt aaattgaaca
    13441 caaaaataac ggtcgaaggt aaagtgtaaa ttaataccgg taagaaaaac attttcgtcg
    13501 gtatcgctct caagaggaac agttgtcgat ggagctccct cggcggggct gaaagtaaaa
    13561 gagccctcgc tttcggagta ttttttacca aaacctatcc agggaaaaat acgaagattt
    13621 gtcgattcac cgaagattac gttttggcca agtcgggcgt aaggtgttac aacgctttga
    13681 tccattttaa agtcatcgcg tgatgtgtcg gtagaaagat agttcattcc ggcgccaaca
    13741 gccaaagaac caatcgtaaa ggagtaggga taaaagtccg cgataaaatg gttgcccgtt
    13801 attttagtat cattaatgtc gggggagtgg tacagaaaat tgttaatttg aaacagttca
    13861 gggttaataa cctgggtgta gagtccgaaa acagatgcgg tttcttccag ttttatgttg
    13921 ttttcaagat tttgcgtatt gctgctgaat ccgccatagg ggccagctaa aaacatggtt
    13981 tccgcgtttg cggtaacaca aaaaaaaata aagatgctta atagtttttt caaaattttt
    14041 cctttcagga tcagcaaaat aatctattta tcggctgatc ctgtttagct taaaa
//
LOCUS       DAOTGZ010000051        14065 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_3064
            NODE_5209_length_14065_cov_5.517773, whole genome shotgun sequence.
ACCESSION   DAOTGZ010000051 DAOTGZ010000000
VERSION     DAOTGZ010000051.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868088
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 14065)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 14065)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:22:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,785
            CDSs (total)                      :: 2,751
            Genes (coding)                    :: 2,732
            CDSs (with protein)               :: 2,732
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 12 of 19
            Pseudo Genes (internal stop)      :: 5 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14065
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5209_length_14065_cov_5.517773"
                     /isolate="AMR_MDS_3064"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(9..146)
                     /locus_tag="P5123_05065"
     CDS             complement(9..146)
                     /locus_tag="P5123_05065"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRX15661.1"
                     /translation="METGLFTCGIITPSSTFPSLFSGFTLQTSGKDYHRTVLITHKYR
                     P"
     gene            complement(306..611)
                     /locus_tag="P5123_05070"
     CDS             complement(306..611)
                     /locus_tag="P5123_05070"
                     /inference="COORDINATES: protein motif:HMM:NF013961.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglutaminase-like domain-containing protein"
                     /protein_id="HRX15662.1"
                     /translation="MKKIIYADYDNILVKETAERLTQNQNSTREKVENIFYYVRDEIK
                     FSFPLKGDLVKASETIKSKKGQCNTKSALFLALCKAAGIPAKIHFSLIKKEIQKGLC"
     gene            complement(720..854)
                     /locus_tag="P5123_05075"
                     /pseudo
     CDS             complement(720..854)
                     /locus_tag="P5123_05075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015675757.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
     gene            996..1250
                     /locus_tag="P5123_05080"
     CDS             996..1250
                     /locus_tag="P5123_05080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019499482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toxin"
                     /protein_id="HRX15663.1"
                     /translation="MIKWNDDKSKFLQFERGVSFEEIAVKLADNDIIDIIENQNFPNQ
                     MIFVMEINEYIYAVPFVMDGIDVFLKTIYPSRKLMKKYRG"
     gene            1252..1545
                     /locus_tag="P5123_05085"
     CDS             1252..1545
                     /locus_tag="P5123_05085"
                     /inference="COORDINATES: protein motif:HMM:NF023857.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CopG family antitoxin"
                     /protein_id="HRX15664.1"
                     /translation="MKKVILSKDETDIENNIESYVSVSEKKRERILAALQQPKVSITL
                     RVDSNDLEKIKTLAEKEGMPYQTLISSVLHKYINNSLVDERSIKKAVSILSEK"
     gene            complement(1648..2664)
                     /locus_tag="P5123_05090"
     CDS             complement(1648..2664)
                     /locus_tag="P5123_05090"
                     /inference="COORDINATES: protein motif:HMM:NF012742.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000976 - transcription cis-regulatory
                     region binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HRX15665.1"
                     /translation="MTTQKDVAERAGVSFITVSRVINNKGNVKQKTRERVLKVIEELN
                     YYPNTLAQGLNANKSNTISILPLLREIAMMEEISFYRRIIAGVERYCCESEIDILLST
                     LRGDPEKIDYLRHYYLRKSDGLILIGISPDRLIDKIEKDKIPCSFIGEKGRPGRANYM
                     HSDNRLGSRQITEYLIKNGHTKIAYIFAENPSADIIERYESFIEVMEENRIAFPKEYL
                     LYGDYMEKGGRKAIRKIAAMRDKPTAIICATDTMALGVFDEAKKLGIDIPNDISLVGF
                     DGHEFCQFMTPPIATVIQDLEGLGYLAAKLTIEQINDPEKKGESHIFPVIFEPRGTVK
                     SLID"
     gene            complement(2903..3985)
                     /locus_tag="P5123_05095"
     CDS             complement(2903..3985)
                     /locus_tag="P5123_05095"
                     /inference="COORDINATES: protein motif:HMM:NF012377.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0071704 - organic substance metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cellulase family glycosylhydrolase"
                     /protein_id="HRX15666.1"
                     /translation="MDINSQLKGVNLGGWFSQIDAIEEKDPEHFPGIDEHIKNFIGQE
                     DIEYIKNSGYNHVRVPIDYQVFFDENGNIRFKDRLLYLEKMIKVILGKDLLIMLDLHE
                     CPGHDFDTGNRQIQRFFTEKEPVQATLKIWKQLAEIFSFDKRIYFEILNEPVAEDNLV
                     WDRLKDQLVEEVRHFAPDQTLIVGSNRWSNVNSFKDFTPIENDENIIYNCHFYNPLIF
                     THQFASWVHNPECHIKREYPGTYTPEGEIFGQVSEKDYGLWNRDRLKETLMPVLEWRA
                     KYNLPVICNEFGVFHQAPRQSQLNYLKDIISILQEVGIGWTYWNYKNLDFGIISIGEQ
                     LHADLPQFQNAQRRDMELAEYLTGVI"
     gene            complement(4049..4918)
                     /locus_tag="P5123_05100"
     CDS             complement(4049..4918)
                     /locus_tag="P5123_05100"
                     /inference="COORDINATES: protein motif:HMM:NF013806.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRX15667.1"
                     /translation="MKKTQLFFPMGLVIAIILYFSCVADDTKSVTAPGASLKDVEALC
                     GDWAVITDGIYKYENNTWGVNSSIRYQQCLITNKKTKKRGWTWTFSGKAPNWVYSYPE
                     IIVGWKPWGQSTSTHKDYPVKLSDIKKLEIRYDVDLTTTGSYNLAPEIWLVREKPVSI
                     PMQKPEALISTEIMFWMDYSSDARPAGSKVGSFKFGGKEYDLWVKADHNNSGDTVSWQ
                     IYSLVSKEKQLKGTIDVIGLVKALRDKGYKINMSEYVASVEFGNETIGPKPNSKAAGT
                     TWINKYEVELVKK"
     gene            5271..5799
                     /locus_tag="P5123_05105"
                     /pseudo
     CDS             5271..5799
                     /locus_tag="P5123_05105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000188008.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="NYN domain-containing protein"
     gene            5837..6040
                     /locus_tag="P5123_05110"
     CDS             5837..6040
                     /locus_tag="P5123_05110"
                     /inference="COORDINATES: protein motif:HMM:NF013691.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRX15668.1"
                     /translation="MYILECCDSTDYTGNKKDLNRLTVLHHQGEGANYTSKRLPITLV
                     YYEEFDGIDESFYREKPIQGWDK"
     gene            6366..7364
                     /locus_tag="P5123_05115"
     CDS             6366..7364
                     /locus_tag="P5123_05115"
                     /inference="COORDINATES: protein motif:HMM:TIGR01426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016758 - hexosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HRX15669.1"
                     /translation="MSKVVFLNIPAHGHTNPTLGIVRELIARGEEVIYFSFPEFKDKI
                     EHTGAKYREYKGFPQSYNTKQSYAFIKTLNFIRHLRCKDIVYLKEGKRIQNLLEKKYN
                     LQKKDFLSTLINTSDLNLIYTSRSLQPQSEKLCSEKYIFIGPSHSYNNNYSNKSEYSS
                     LKKPIIYISLGTILHNNPEFYKHCFEALDDFEGSVIISIGTETKINDLGNIPKNFVIK
                     SYVNQIEVLKQSKLFISHGGMNSVHESLLYAVPLCVYPFHVEQEAVAERIKETGCGII
                     LKKLNSKEIKKAVEKICKDDSYQTNCKKQSDNLIKAGGYKTGVDSILTFKEKNVSV"
     gene            complement(7423..8142)
                     /locus_tag="P5123_05120"
     CDS             complement(7423..8142)
                     /locus_tag="P5123_05120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012763076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NYN domain-containing protein"
                     /protein_id="HRX15670.1"
                     /translation="MKNNLEYYHHRTAILVDGAFFIKRLRYLHSKKSNYDPYSAKNTI
                     KDLYRMVQSHLKHCNGLLYRIFYYDCPPLTKRLHNPINNKPIDLSQSRQAVFMNELFV
                     ELKKSRKIALRLGKLNDPGYWRIKDKKTKLLLQKKIQVDQLLENDVEWSVNQKQVDMK
                     MGLDVASLAYKQLVDQIVMIAGDSDFVPVAKVARREGIDFILDPMYKSISPDLSEHID
                     GLYSTYGKKKTEKKCQENSLE"
     gene            complement(8304..9227)
                     /locus_tag="P5123_05125"
     CDS             complement(8304..9227)
                     /locus_tag="P5123_05125"
                     /inference="COORDINATES: protein motif:HMM:NF015722.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SUMF1/EgtB/PvdO family nonheme iron enzyme"
                     /protein_id="HRX15671.1"
                     /translation="MQSIIKHQESENSSVFKLFVIIACFIILTSCSKSVPQSAVFVQG
                     GKYRTGDGINHAQYEVEIDDFYMLDHEVTNSEFADVLNWALNKKMIYVNEEHKRVLLN
                     QDFPWDLIELYKEGCQIKLENNKFSVIDGFEDYPVIFVSWYGAVVYCNFLGLKEGRNE
                     LYDFNDWVYDRNAVNGYRLPTNEEWEYAATERGKSAKIFSGAANPEKCAWYKTNSGGS
                     THPVKQLEPNELGLYDMSGNVWEFCTEKMGDFRGQIQTPELSLNTSYRLIRGASFLSE
                     DINLYRFVQIIDYPNYLKGSEETGFRTVYVK"
     gene            9413..10546
                     /locus_tag="P5123_05130"
     CDS             9413..10546
                     /locus_tag="P5123_05130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004073943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HRX15672.1"
                     /translation="MSQVKRIYIPCFEKDSKKKALVITGPRQCGKTTAANMLTGTKEY
                     FNYDLQEDRDIITQKSWNRTTDYIIFDELHKMPGWKQFIKGLIDTESNNPSLIITGST
                     CLYTYKKFGESLAGRYFEYRMHPLDVREICLMDDSADPDFVFDKILRFSGFPEPFIEA
                     DERFYNKWKKTHLDIIIKQDIVSYESVRNIASIELLVDLLRKKVGSPISYISLAEDLK
                     VSDKTVKHWLNILEEMFIIFKITPYHRNIARAVQKKPKYYFYDCMRVASGDGAKIENA
                     VASSLFKECQFREDCLGETWQINYLSKRGGVEVDFLITKEGVPHTMIEVKKSDDSLSK
                     NFSSFTELLPDVQIVQLVKDLRKEKTYPSGAMVRRAGSWLAKW"
     gene            complement(10589..11503)
                     /locus_tag="P5123_05135"
     CDS             complement(10589..11503)
                     /locus_tag="P5123_05135"
                     /inference="COORDINATES: protein motif:HMM:NF024676.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="WYL domain-containing protein"
                     /protein_id="HRX15673.1"
                     /translation="MLDKKLFGKTTQIDILRRYLHIIALLQNKDDKWNNGTLAETLSK
                     DPNSNSDIYDTHIKHCTEEYIENELGLTLTKRKGARSFTFENPIEKDLLLRLINIYSI
                     FVIEDIQKDIALQSLVEKYPEQCLWLLGRLFFAKIEKNVIEFKYTNNKGSNQKVACHP
                     YHIIFKNNNLYLVALREYDGLVCLYIVNRINDLKVKDKKFIEEIPDSASYFKNSISSY
                     ISNKPAIKVILKFKKELRNNINDILSGIEAEIEDKDDHCIAECLLNDTMYLCKQLFLY
                     GNGVEIIKPDSIRNEMTAMLKKSLSVYQ"
     gene            11717..13948
                     /locus_tag="P5123_05140"
     CDS             11717..13948
                     /locus_tag="P5123_05140"
                     /inference="COORDINATES: protein motif:HMM:NF012234.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HRX15674.1"
                     /translation="MQHNHFSAQHCGLSVHHITVSYYKNMFERNLVTDQIIDYIFESF
                     FRTEKRRCLFIREFKNEFLSEIEYWEKISPRGYLSEDILRELDDVYTRSLNWSSLMRT
                     IPTCGRKKVTTFIRSFLLDILEEELKMSSSDRAAAIRREEELASVFSLQKNEIELLKY
                     CIAVELDEYDFENSIASLGNSRRSSIKTASQITGLDQAEISYLMKSKNNLLKSGVLSQ
                     CSRSRNSLGVDVDESVLQFASGLEAVDSIMNSYIYIDRKKPLSLRSFTTKKEYSEIIK
                     DILNCSGPANILLHGRAGTGKTEYARSLVRSVKKRPVFVTTRKSSSGRGSRQIALEAA
                     INIVTENDVLIVDEADYLINSMGFFRTPHQDKGWINDLLDRSNVKMIWITNHTQNVEE
                     SHLRRFSFSLLFDSFGSAERKKIWKNIVGHGPLASIIDSEKIEHYSNEYEVNAAGIAS
                     ACEAASSIIKENTKKKKNVLAIIENILEQHEQLIKGRPHKKERLFNIAAEYNVDFINT
                     SLPADDLVEPLSQIANFIRTKSDKEYNANILLYGIPGTGKTEFVKYLASQLKLKICLK
                     RASDLLSPYVGETEMLINRAFREAEKSGQILFIDEADTFFKERTAFSRSWELSQTNEL
                     LSQMENFKGILICATNMKSLLDSAAIRRFNFKVQFNALKTDQYLPVYKDYFSKLTGLP
                     KKRDLDKLMTISELTLGDFRAVANAYKFVAKKDITPAEIIGRLAQESSHRDDKKEKQV
                     GFA"
BASE COUNT         4456 a         2544 c         2508 g         4557 t
ORIGIN      
        1 ggtggtcttt atggacgata tttatgagtg atgagaacag tacgatggta gtcttttcct
       61 gatgtttgca gtgtgaaacc ggaaaacagg gacggaaaag tggacgaagg tgtaataata
      121 ccacatgtaa ataatcctgt ttccataatt ctcctcctgt ttactaataa tgctaagata
      181 agaagatgaa ctcaattagt gtactgtatg cctacatatt atccgtcccc gtttcaaata
      241 ataccaccgt ccccattaag actacgattg gcagcgtaaa ttttcagtaa tttagtaatt
      301 tagaatcagc agagcccttt ttgaatctct ttttttatta gtgaaaaatg tatttttgca
      361 ggaataccgg ccgctttaca gagcgcaaga aatagtgccg attttgtatt acactgtccc
      421 tttttacttt taattgtttc agatgccttt acaagatcac ccttgagtgg aaagctaaat
      481 tttatttcat ctctgacgta ataaaagata ttctcgactt tttctcgggt agaattttga
      541 ttttgagtaa gtctttcggc agtttctttt accagtatgt tgtcgtagtc cgcataaata
      601 atctttttca taagtatggg ctcctttgtt taatttctat tggaacatag cctatttaaa
      661 agaatctgac ttgaatgaac ctgctgtttc ggaggatttt ataaaaaagc ggtcagcgat
      721 tatttaaata aattttgtag agttaaacca aacatcttct tgaacgtgcg gctaagatga
      781 gccgaatccg aaaagcccgc ctgatgagct gcaaaagtga aatctttgcc ttcaaatatc
      841 gtctgtattg caattatgaa gtaacccgaa taaaaatgaa actccaaaag attcaaaact
      901 gataaattca aaaaataata cttttagtag atgcgaactc gaccaaagga ttaaaaagga
      961 ttgatattgt acgtacattg taatatattg cttctatgat aaaatggaat gatgataaaa
     1021 gcaaatttct ccaatttgag cgaggagttt catttgaaga aattgctgtt aaattggcag
     1081 ataatgatat tatagatatt atcgaaaatc agaattttcc aaatcagatg atctttgtaa
     1141 tggaaataaa tgagtacatt tacgcagttc cttttgtcat ggatgggatt gatgtttttt
     1201 taaaaacaat ttatccaagc agaaaattga tgaaaaaata caggggataa tatgaagaag
     1261 gtgattcttt caaaagacga aacagatatt gagaataata ttgaatccta tgtatctgtt
     1321 tcagaaaaaa agcgggaaag aattctggct gctttacaac aacctaaagt ttctataacc
     1381 ttacgggttg atagtaacga tcttgaaaaa atcaaaactt tagcagaaaa agagggtatg
     1441 ccgtatcaga cgttaatttc aagtgtattg cataagtata taaacaattc attagtagat
     1501 gaaagatcca taaagaaggc ggtttccatt ttatcagaaa agtgatttaa tgaattgaat
     1561 atttcagcat tatgatcaaa ccttatttac tgcgaattac agattttggg gaattattaa
     1621 ttattgtgag acatgatttt atataaatta atcaataagg ctctttacag tacctcgagg
     1681 ttcaaaaatt accggaaaga tatgtgactc gccctttttt tcgggatcgt ttatctgctc
     1741 tattgtaagt ttagcggcca ggtagcccaa tccctcaaga tcttgaatta cagtcgcgat
     1801 aggtggtgtc ataaattggc aaaattcgtg accgtcaaac ccgacaagag atatatcgtt
     1861 agggatatca ataccaagtt tttttgcctc gtcaaagaca ccaagcgcca ttgtgtctgt
     1921 tgcacagata atagcagttg gcttgtctct catggccgct atttttcgta tagcctttcg
     1981 accgcctttt tccatatagt cgccataaag caggtactct ttcggaaaag ctatcctgtt
     2041 ttcttccatc acttctataa aactttcgta tctttcgata atatcggccg aagggttttc
     2101 cgcgaaaata taagctatct ttgtgtggcc gttttttatc agatattctg taatttgacg
     2161 gcttcctaac cggttatctg aatgcatata attagctctg ccaggacgac ctttttctcc
     2221 aataaagctg catggaattt tatctttctc gattttatcg attagtctat ctggagaaat
     2281 gcctattaat ataagcccat ccgactttcg caggtaataa tgccgaagat agtcaatttt
     2341 ttctggatca cctcgtaatg tagaaagtag tatatctatt tcactctcac aacagtaacg
     2401 ttcgacaccg gcaatgattc gccgataaaa cgatatctct tccatcattg ctatctctct
     2461 taataatgga agtatcgata ttgtatttga tttgtttgcg ttaagaccct gcgctaaagt
     2521 gttagggtag tagtttagtt cttctattac tttgagtact cgctctcgag tcttctgttt
     2581 gacatttccc ttattgttaa taacgcgaga aacagttata aaagaaactc cggccctttc
     2641 cgctacatct ttttgagtcg tcatttttac accatttatg atattctata tatcatctta
     2701 attaaaaacc ttagttcgga taatttccga accattattc tctttaacat gtagatgaat
     2761 caaacaatat ttgtgaaaat cctaaattat tgtttattaa atcaggatgc ataatgcatg
     2821 tgtcgtgcaa gattgttctg ttttagcaac atagcgtcta aagaccagca gagtaactaa
     2881 tatattaata caaaatgttt atttaaataa cgcctgttag gtattccgca agttccatat
     2941 ctcgtctttg tgcgttttga aactggggaa ggtcggcatg aagctgttcg ccaatggaaa
     3001 ttatcccaaa atcgagattt ttgtagttcc agtaggtcca gcctattcca acttcctgaa
     3061 gtatcgaaat tatgtctttt aaataattta actgcgattg ccttggagcc tggtgaaaga
     3121 cgccaaactc attgcagatt acaggcagat tatattttgc tctccattca agaacaggca
     3181 taagagtctc tttaagacga tcccggttcc acaagccata atctttttca cttacctgac
     3241 caaatatttc tccctcggga gtgtatgtgc cgggatattc tcttttaata tgacattccg
     3301 ggttatgtac ccatgatgca aactgatgag taaaaataag aggattgtag aagtgacagt
     3361 tatagataat attttcgtca ttttcaatcg gagtaaaatc cttgaatgag tttacattgc
     3421 tccatctgtt tgatccgaca attaatgtct ggtctggagc aaaatgccgt acctcctcaa
     3481 caagttgatc tttgagacga tcccatacta gattatcttc tgccactggt tcattaagaa
     3541 tctcaaaata gatgcgttta tcaaatgaga atatttcggc aagttgcttc catattttta
     3601 aagtagcctg taccggttct ttttctgtaa aaaaccgttg tatctgcctg ttgccggtat
     3661 caaaatcatg accgggacat tcgtgaagat ccagcataat taatagatct tttccgagta
     3721 tcacttttat cattttttca agatacaaaa ggcgatcttt aaatcggata ttgccgtttt
     3781 catcaaaaaa aacctgataa tctataggaa cacggacatg attgtatccg ctgtttttta
     3841 tgtactcaat atcctcttgt ccgatgaagt tctttatatg ttcgtctatt ccgggaaaat
     3901 gctccgggtc tttctcttct atcgcgtcaa tctggctgaa ccagccccca agattaacgc
     3961 cctttagttg tgagttaata tccattgcgg tttctccgtt tgaatttctg aattcaggaa
     4021 ttcatttaat ataatctgta ttaaaaaact atttttttac aagctctact tcatatttgt
     4081 taatccaggt tgtaccggca gccttgctgt ttggctttgg gccaatggtc tcgtttccaa
     4141 attccaccga agcaacgtat tcgctcatat taatcttgta tcctttgtca cgtaatgctt
     4201 taaccaggcc tataacatca atagtacctt ttaactgttt ttctttgctt acaagtgaat
     4261 aaatctgcca cgagacagta tctccggagt tgttgtggtc cgcctttacc cagaggtcat
     4321 attctttccc accaaattta aaactgccga cttttgaacc tgctggccgg gcatcgcttg
     4381 agtaatccat ccagaacata atttcggtag atatcagtgc ttcaggtttt tgcatcggaa
     4441 ttgacaccgg tttttctctt accagccaaa tttcaggggc aagattataa ctgccggttg
     4501 ttgttaagtc tacatcataa cgtatttcaa gtttttttat gtcagagagc ttcacggggt
     4561 aatccttatg tgttgatgtc gattgccccc atggtttcca gcctacgatt atttccgggt
     4621 acgagtagac ccagttcgga gcctttccgc tgaaagtcca tgtccagccc cgtttttttg
     4681 tctttttgtt tgtgattaga cactgctggt agcgaatact gctgttaaca ccccaggtgt
     4741 tgttttcata cttgtatatc ccatcggtaa taacagccca gtcgccacac aatgcttcaa
     4801 catcttttaa agaggctccc ggagcagtta ctgacttagt atcatctgca acacaactaa
     4861 aatacaaaat tatggcaatt actaatccca ttggaaaaaa taattgagtc tttttcataa
     4921 tttattctcc tttactatgt ttgcgaaaac attaatattg tacaggtaat tcgtcaaggt
     4981 tttttgattg ttggaggtaa atggtgtggg tgaaacactt ggatttgttt ctttttgagc
     5041 acaacaattg ttaattttca ttgttcattg ccttatctca ttggcattct tcctcttata
     5101 gatgggtttt atccagtcgg tctttattga ttcaattgta attaatatta aaaaaaacat
     5161 tgacactgtc gataagggta atactatata caacatatca ccccgactgc ttcggcggcg
     5221 ggtgcaagac tacctttcgg ggtggtcttc gcttttttat ggggtaatta atgacccagc
     5281 taagaacctc aatttacatc gacggcttta acctctatta cggtttacta aaaaacaccc
     5341 cctataaatg gctcaacata gaatcccttt gccaggcata ccttaatcct cagcttaata
     5401 atattcaaaa aatcaaatat ttcaccgcgc ttgtcaaatc ccgtccgaat gatcccgatc
     5461 agcatatccg tcagcaagca tatataagag cattgaaaac actgccctgt cttgaaataa
     5521 tttacggtac gtttcttacc cacgaaataa caatgccgcg agcagacggt actggtttca
     5581 aacgattctg atctctcaga gccgcttcga ataatcaagg atgaactgaa aaaagtcata
     5641 ggtattctta atcctcaaaa aaacccgagt aatgaattac gaaaatatac aaatttctta
     5701 aaaaaaatac gatcaggggc actcccttct tgtcagtttc ctgatacttt acaggataca
     5761 catggcataa taaaaaaacc ggatgaatgg aaacgatgag cgtacagaga atggatgaaa
     5821 tattataaaa gggtatatgt acatccttga gtgttgtgat agtaccgatt atacaggcaa
     5881 taaaaaagat ttgaatcgtt tgactgttct gcatcatcaa ggtgaaggtg caaactatac
     5941 aagtaagagg cttccgatta cacttgtgta ttatgaagag tttgacggga ttgatgaatc
     6001 cttttataga gaaaaaccaa ttcaagggtg ggataagtaa tttttttatt accgattatg
     6061 cttagtttat gacatgattt atcgaccata ccgttttcac ttgcataatg acctccaacg
     6121 ataaatcgat taagataaga ttaacaggac aaaggatata aatcgatcgt actcataaat
     6181 tcgtataatt aaatggatga agatgagaaa gttatctacg ttatacgaca tcaaaggaga
     6241 ctaatcagag tttgtcttga caactaaact aattttatat aaggttctca ttacaatttt
     6301 atcctgcaaa tttgtaatga gttttatcta ataatgatgt tttgttaggt aatcaggatg
     6361 gctctatgag caaagtggtt ttcttaaata tcccagccca tgggcatacc aatccaacac
     6421 tcggcattgt tagagagtta attgcaagag gagaagaggt gatctatttt tcttttccag
     6481 aatttaaaga taaaatcgag catactggcg caaaatacag ggagtataaa ggtttccctc
     6541 aaagctataa tacaaaacaa tcttacgctt ttatcaaaac tttgaatttt atcagacatt
     6601 taagatgtaa agacattgtt tatttgaaag agggtaaaag aattcaaaat cttttggaaa
     6661 aaaagtataa cctgcaaaaa aaagattttt tatctacact aataaacaca tcagatctta
     6721 atctgatata tacttccaga agcctacagc ctcaatcaga aaaactatgt tctgaaaaat
     6781 atatttttat cggcccttct catagttata acaataatta ttcaaataaa tctgaataca
     6841 gttctttaaa aaagccaatt atttatattt cattaggcac aatattacac aataatcctg
     6901 aattttataa acactgcttt gaagcattgg atgattttga agggagtgta attatatcga
     6961 ttggaacaga aacgaaaatt aatgatttag gaaatattcc aaaaaatttc gtgattaaaa
     7021 gttatgtaaa ccagatcgaa gtattaaaac aaagcaaatt gtttatcagc cacggtggaa
     7081 tgaacagcgt tcatgaatca ctattgtatg ctgttcccct ttgtgtttat ccatttcatg
     7141 tcgaacagga agctgtcgca gaaaggatta aagagacggg ttgtggaata atactgaaga
     7201 aattaaactc taaagaaata aaaaaagctg tagaaaaaat atgtaaagat gatagttatc
     7261 agactaactg caaaaaacag agtgataatt taataaaagc cggtggatat aaaactggtg
     7321 ttgattcaat ccttactttt aaagaaaaaa atgtatctgt atagtatatg gaagactgaa
     7381 ttatatgtcc ggaaatagga cagccccctt agaactgtaa agttattcta agctgttttc
     7441 ctggcatttc ttttcggttt tctttttacc atatgttgaa tataagccgt caatatgttc
     7501 gctcaaatca ggtgaaattg atttatacat tggatcaaga atgaaatcaa taccttctcg
     7561 ccgcgcaact ttcgcgactg gtacaaaatc actatctcca gcaatcatga caatttgatc
     7621 aacaagttgt ttatatgcta aagatgcaac atctaaaccc attttcatat ccacttgttt
     7681 ttgatttaca ctccactcaa catcattttc gagtaattga tcaacttgta ttttcttctg
     7741 caaaagaagt ttcgtttttt tatcttttat gcgccaatat cctggatcat ttaactttcc
     7801 aagtcttaaa gcaattttac ggcttttttt caattcaaca aatagttcat tcataaaaac
     7861 agcttgtcta ctttgggaca gatcaatagg tttattatta attggattat gaagtctttt
     7921 tgttaatggt gggcaatcat aataaaaaat tctatataat agaccattgc aatgcttgag
     7981 gtggctctgt accattctat acagatcctt tatggtgttt tttgcactat acggatcata
     8041 gttgcttttt ttactatgca gatatcgtaa tcttttaata aaaaaagcgc catcaaccaa
     8101 aattgcagtt ctatgatgat aatattctaa attatttttc atttctacgt gaaaaacaga
     8161 aagcctccgg gtactttaca catcaggatg tttgtgtgga tttctccgaa ggcactatgt
     8221 tctcctaaca atatcgtgat tatttagttt cttttcaagc atttttcaaa tattattatc
     8281 aataatctta attataataa tatctactta acatacaccg ttctgaaccc cgtctcttct
     8341 gatcctttca gatagtttgg ataatcgata atctgtacga acctgtacag atttatatct
     8401 tcagacagga aacttgctcc ccgtatcaga cgatatgagg tgtttaagct taactccgga
     8461 gtctgtatct ggccacgaaa atcgcccatt ttttcggtac agaactccca gacattaccg
     8521 ctcatatcgt ataagcctaa ttcatttggt tcaagctgtt ttaccggatg agtgcttccg
     8581 ccgctattag ttttatacca ggcacacttt tccggattag ccgcacccga gaaaatcttt
     8641 gccgattttc cgcgttcagt tgccgcgtat tcccactctt cgttagtcgg caagcggtat
     8701 ccgttaacag cgtttctatc gtaaacccag tcattaaaat cgtataattc gttccgccct
     8761 tctttcagac caagaaaatt acaatacaca accgcgccat accatgaaac aaatattacc
     8821 ggataatctt caaacccatc tataaccgaa aacttgttat tctccaattt tatctgacag
     8881 ccctctttat acagttcaat taaatcccat ggaaaatcct ggtttagcaa aacacgcttg
     8941 tgctcttcgt ttacataaat catcttttta tttaaggccc aatttaaaac atccgcaaat
     9001 tcagaatttg ttacttcgtg atcaagcatg taaaagtcat ctatttcaac ttcatactgt
     9061 gcgtgattta taccgtcacc ggtacggtat ttgccgcctt gaacaaacac agcagattgt
     9121 ggaactgatt ttgaacagga tgtcaggata ataaaacagg ctataattac gaataattta
     9181 aaaaccgagc tgttttctga ttcttgatgt ttaataatgc tttgcatgat aacctccgtc
     9241 aatattgcga ttaaaacgag attaacagaa aaaaggaggt caatcgaccg tactcataaa
     9301 ttcgtacaat taaagagatt aaaatgcgaa aataatctat tttgaacgac attttcggag
     9361 tctactccga gtttgtcttg acattttcgg agtagactcc gaaaatgtca agatgtcaca
     9421 ggtaaaaaga atatatatac catgctttga gaaggacagt aaaaagaagg ctcttgttat
     9481 aactggcccg agacagtgtg gaaaaacaac tgcagcaaat atgcttactg gaacaaagga
     9541 atattttaat tatgatctcc aggaagaccg ggatattatt acacaaaagt cctggaacag
     9601 aacgacagat tatataatat tcgatgaact acataaaatg cccggttgga aacaatttat
     9661 aaaaggactt attgatacag aaagtaataa tccgtctctt ataatcaccg gaagtacatg
     9721 cctatataca tataaaaagt tcggagagtc tttagctggt cgctattttg agtatcgaat
     9781 gcatcctctg gacgtacgtg aaatttgtct gatggatgat tctgcggatc ccgattttgt
     9841 ctttgataaa attttacgtt ttagcggttt tccggaacct ttcattgaag cagacgagcg
     9901 gttttacaat aaatggaaaa aaacccatct tgatataatc attaaacagg atattgtatc
     9961 ttatgagtct gtgcgaaata ttgccagtat tgagcttcta gtggatcttc ttcgtaaaaa
    10021 agtaggttca ccaatatcct atatttcgct tgccgaagat ttaaaagtaa gtgataaaac
    10081 tgtaaaacac tggctcaata ttcttgaaga gatgtttata atttttaaga ttacaccata
    10141 tcaccgaaat atcgcaagag ccgttcaaaa aaaacccaag tattactttt atgattgtat
    10201 gcgtgttgct tctggggatg gggccaaaat tgaaaatgca gtggcatcat cgttatttaa
    10261 agagtgtcaa tttcgtgaag attgtctggg cgaaacgtgg cagatcaact atttatcaaa
    10321 gcgtggaggg gtagaagttg attttttaat caccaaagaa ggtgttcctc atacaatgat
    10381 agaagtaaaa aaaagtgatg actcactttc taaaaatttt agctctttta cagagctact
    10441 acctgatgta caaattgtgc aacttgtaaa agatctcaga aaagaaaaaa cctatccatc
    10501 aggagctatg gtgcgtcgtg ccggttcttg gttggcaaaa tggtaacatg ttactgacaa
    10561 tctctaataa ttaacttttt gaagttccct attggtaaac actaagactt ttctttagca
    10621 ttgcagtcat ctcgtttcta atggagtccg gtttaataat ttcgactccg ttcccatata
    10681 aaaagagctg tttacacaga tacatggtat cgttaagcag acactctgcg atacagtggt
    10741 catctttatc ttcaatttcg gcctctattc ctgaaagaat atcatttata ttattgcgca
    10801 gctctttttt aaatttcaag ataactttta tagcgggttt atttgaaata taagagctaa
    10861 tagagttttt aaaatagctt gccgaatctg gtatctcttc tataaatttt ttatccttta
    10921 ctttaagatc gtttatacgg ttaactatgt aaaggcatac caatccatca tattcacgaa
    10981 gagcgacaag atacaggttg ttatttttaa atataatatg ataggggtgg caagcaacct
    11041 tttgatttga acctttatta tttgtgtatt tgaattcaat aacatttttc tctattttgg
    11101 caaagaataa cctgcccaaa agccaaagac actgctccgg atatttttcg acaagagatt
    11161 gtaacgcgat gtccttttgt atatcctcta taacaaatat ggagtagata ttaatcagcc
    11221 ttaacaataa atctttctca atagggtttt caaaagtaaa actgcgcgct cccttgcgtt
    11281 ttgtcagagt aagccccaac tcattttcta tatactcttc ggtacagtgt ttgatatggg
    11341 tatcataaat atcggagttg ctgtttgggt ctttggacaa agtttcagcc aaagtgccat
    11401 tgttccattt gtcatccttg ttttgtaata gggcgattat atgtaaatat ctgcgcaaaa
    11461 tgtctatctg ggtcgttttg ccgaatagtt tcttgtctaa catgagctcc tccgtttgct
    11521 ataattttct aaaatataaa atatggagta actccggtca agctaaaagg ggacttaatc
    11581 tatttgtagt ttttcttgcc gtttttttca taaaataact taaaaaactg atttttttta
    11641 caaggtagat tctcagtcgt taccttgcaa ctacctgtta ttgtatcttt gtaacataca
    11701 atatcggagg tatgctatgc aacataacca tttttcggct caacactgtg gactttctgt
    11761 tcaccatatt actgtaagtt attataagaa catgtttgag cgaaatctag ttacagatca
    11821 gattattgac tatatttttg aatctttttt tagaaccgaa aaacgcagat gtttatttat
    11881 aagggaattt aaaaatgaat ttttatcaga aattgagtat tgggaaaaga taagcccaag
    11941 aggttatctc tcagaggata tactgagaga attggatgat gtttatacaa gaagtctaaa
    12001 ttggagcagt ttaatgcgta ctattcccac ctgtggtcga aaaaaagtca ccacctttat
    12061 tcgatcattt cttttagata ttctggaaga ggagctgaag atgtcatcat ctgatagagc
    12121 agcggctata aggcgtgaag aagagttagc atcggttttt tcgttgcaga aaaatgagat
    12181 agaactgcta aaatattgta ttgccgtgga gctggacgag tatgattttg aaaattccat
    12241 cgcctctttg ggtaacagtc gtcgcagcag tattaaaaca gcctctcaaa ttaccgggct
    12301 cgatcaggca gaaatcagct atttgatgaa atctaaaaat aatcttttaa agagtggcgt
    12361 tttatcccag tgcagccgta gccgaaattc tcttggagta gatgtcgatg aaagtgtgct
    12421 gcaatttgca tccggtttag aggctgtgga ttcgataatg aattcttata tttatattga
    12481 tcgaaagaag ccgctttcac taagatcatt tacaacaaaa aaagaatatt ccgaaataat
    12541 caaagatata cttaattgca gcggtccggc aaatattctt cttcatggtc gcgcgggaac
    12601 cggtaaaaca gaatacgcgc gatcacttgt gcgaagcgtt aaaaaacgac cggttttcgt
    12661 aactacaaga aaatcttcat caggtcgcgg cagtcgacaa atcgctcttg aagctgcaat
    12721 caatattgtt accgaaaatg atgtacttat tgttgatgag gccgattatt tgatcaattc
    12781 tatggggttt tttagaaccc cccaccaaga taagggctgg ataaacgatc tgcttgatcg
    12841 ttcaaatgta aaaatgatct ggataacaaa ccatacacaa aatgtcgaag aatcacatct
    12901 tcgtcgtttc tcgtttagtc tgctttttga tagctttggc tcggcagaaa gaaaaaaaat
    12961 ctggaaaaac atagttggcc atggaccact cgcgtccatt atagatagtg aaaaaattga
    13021 gcactattct aacgagtatg aagtaaatgc tgcaggtatc gcttccgcat gtgaagccgc
    13081 ctcttcaata atcaaagaaa acactaaaaa gaaaaaaaat gttttggcta taatcgaaaa
    13141 catactcgaa cagcatgagc agctaataaa aggaagacct cataaaaaag agcgtctctt
    13201 taacattgcc gcggagtata atgttgattt tatcaataca tcacttcctg ctgatgatct
    13261 tgtcgagccg ctttcacaaa ttgcaaactt tatacgaaca aaatcagata aagagtacaa
    13321 tgcaaatatc cttctctatg gcattccggg aaccggtaaa acagagtttg tcaaatatct
    13381 tgcgtcacaa cttaaactta aaatatgttt aaagcgtgcc tctgatttac ttagccctta
    13441 tgtaggggaa accgagatgc ttataaatag agcatttcgt gaagccgaaa aaagtggtca
    13501 aatactcttt atcgatgaag ccgatacatt ctttaaagag cgaaccgcat tctcaagatc
    13561 ttgggagttg agtcagacaa acgaactttt atctcagatg gaaaatttta aaggaatatt
    13621 aatctgtgcc acaaacatga aatctctact tgactccgcc gcgatacgac ggtttaactt
    13681 taaagtacaa tttaatgctt taaaaacaga tcagtattta cctgtttata aagactattt
    13741 ctcaaaacta acaggtctcc ccaaaaagag ggatcttgat aaacttatga caattagcga
    13801 gttaaccctt ggagacttta gagcagttgc aaatgcctat aagtttgtgg caaaaaaaga
    13861 tattactccg gccgagatta ttggtcgcct ggcacaggag tccagtcacc gtgacgacaa
    13921 aaaagagaaa caagtcggtt ttgcataaag agccaacagg tatcccgttt agggatacct
    13981 gttagctcaa tctgtgatca atgtaaaaca gtaaaaattt attgagaggt taatatgata
    14041 ttagaggaat tcgcgggttt aatag
//
LOCUS       DAOTGZ010000052        14013 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_3064
            NODE_5224_length_14013_cov_4.859435, whole genome shotgun sequence.
ACCESSION   DAOTGZ010000052 DAOTGZ010000000
VERSION     DAOTGZ010000052.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868088
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 14013)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 14013)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:22:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,785
            CDSs (total)                      :: 2,751
            Genes (coding)                    :: 2,732
            CDSs (with protein)               :: 2,732
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 12 of 19
            Pseudo Genes (internal stop)      :: 5 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14013
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5224_length_14013_cov_4.859435"
                     /isolate="AMR_MDS_3064"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1202)
                     /locus_tag="P5123_05145"
     CDS             complement(<1..1202)
                     /locus_tag="P5123_05145"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRX15675.1"
                     /translation="MNRKVVCLFIILLSFFSCSRTDYFDFLSARSVKSFSFAAADNGL
                     DGFDIDAVYDKSSLSFVAVFPKDTLPPSGTGMRLVPSFEIDGYALYVDGKRQSSGLDG
                     LDFTDKQSYTYKLYSYDGKSTDYTVTAQNAAAWFESFGFAASDNAILSSTFYNIGDIM
                     VPEDDIEGNAIWFDLPHTVSLDGLVPTFTTSPSDVKVFFVDKNGKEHANVTAPRDFSD
                     YCVYRVYDNRNESYLDYRVSALRLKILSFKNYPEIEACLIDHDMSDNINDVFVQLPYN
                     ADLRALVPVFDVSGENVLFYASDGERVDAVSGETSLDFSDPVRIVVKSRAGKSKEYRI
                     VVSRTESPDIEPYNPPNNPAKPDGSGNESGEPIVPPTGFTLGYNANGGSGSLPARRSY
                     SPGDSIIL"
     gene            complement(1390..1839)
                     /locus_tag="P5123_05150"
     CDS             complement(1390..1839)
                     /locus_tag="P5123_05150"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRX15676.1"
                     /translation="MIATVFMFFFITLAAFALSFAVFFSINYARNAGIRFQDTSGRTL
                     PGSMGGGKSLNYSKTRGKEAGFEFSFTPEALREGLKAKDKKIITQFVILTGFVTFVLS
                     VFAAIGCGLLGSGEYLIGIIFCVVPIIFGAVFVYQLLFGRSPDKTLD"
     gene            complement(1986..3017)
                     /locus_tag="P5123_05155"
     CDS             complement(1986..3017)
                     /locus_tag="P5123_05155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010247379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0071704 - organic substance metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cellulase family glycosylhydrolase"
                     /protein_id="HRX15677.1"
                     /translation="MTAAKTIPRWRGFNLLNYFISEEESKMNGKYYRSDGNFSADEIK
                     WIADWGFNFIRIPMCYRNWLSDSKDMFVMDEIELKKIDRVIELGNANGLHVSLNIHRA
                     PGYCINTEILEPFDLFKDELPVALFNHYWETFAKRYKSISGDMLSFDLLNEPPNPADG
                     KMCREDYVRVMKGAIENIRKVDPDRIIMVEGLSVGTEPVLELTDTGAVQSCRAYAPFG
                     ISHYKAEWVQDYEWQKPVWPGIIENGKPFNQDRLEEHYKGWADLKKNGVAVHCGEGGA
                     YIYTPHDVVLSWFEDVLKILTKHDIGYSLWEFSGEFGILDSGRADVQYEDWHGRKLDR
                     AFLELLRRY"
     gene            3274..3750
                     /locus_tag="P5123_05160"
     CDS             3274..3750
                     /locus_tag="P5123_05160"
                     /inference="COORDINATES: protein motif:HMM:NF021524.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2254 family protein"
                     /protein_id="HRX15678.1"
                     /translation="MQFLRWVNSIARLGRLGNTIDKVEEATLSALQRRLNNQAFGCVP
                     VSTVSTKGYAIKTDSIGYLQYIDTSSLQICCEELSAYLNIAVQPGTFIGPGHILAYLD
                     TENSDNNDFDYTHIHKAFMIGKQRVFDDDPRYGLIVLSQIADRAHFSRSKRSRHSY"
     gene            3698..4102
                     /locus_tag="P5123_05165"
     CDS             3698..4102
                     /locus_tag="P5123_05165"
                     /inference="COORDINATES: protein motif:HMM:NF021524.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2254 family protein"
                     /protein_id="HRX15679.1"
                     /translation="MPTEPISPGVNDPGTAIDIISTFVRLFSIWINFKNDSQESEAKF
                     DKVRFCTLSVKDMFDDAFTAIERDGSSLIEVTIKLIKSLSALSSIGDEQVQQAAAYHA
                     KRIIKYAEQKLVLESDINNVRDEAQIIFSVKP"
     gene            4203..5294
                     /locus_tag="P5123_05170"
     CDS             4203..5294
                     /locus_tag="P5123_05170"
                     /inference="COORDINATES: protein motif:HMM:NF012472.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HRX15680.1"
                     /translation="MIYREYGSTGKMVSALGFGGMRFKDIKDIDTCASMMLTAARGGI
                     NYFDTAPKYFGTGSEEAFAKGLAAMRNENLPYYLSTKTDESHPDSIRREIEAQLKRLD
                     VNHIDFYHIWCITSLDNWRERKKNGVLETFIKLKEEGLIKHICVSSHLIGDEIKELLM
                     EEIFEGVLFGYSAYNFKTRQSAFDAITERRIGCVVMNPLGGGIIPGNPEIFRFLQTVP
                     DETVVQAALRFLFAHQEITISLVGFETEEQVNEALAAANSPLQLSPDKLKQIKKNATV
                     SLEGICTGCQYCDHCPVNIPIPKYMDAYNQKILTDSKTAITDRLFWHWSLKPEDAAAC
                     IECGRCEEECTQHLNIIERLKEISVLPAK"
     gene            complement(5357..7252)
                     /locus_tag="P5123_05175"
     CDS             complement(5357..7252)
                     /locus_tag="P5123_05175"
                     /inference="COORDINATES: protein motif:HMM:NF024598.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative glycoside hydrolase"
                     /protein_id="HRX15681.1"
                     /translation="MKSIAKRSSAFFSSWVIFILFLSFTFILLSNCSNKEYAAVQKRP
                     AYRVHFAQRVKDNIYIATYDQGLFVKSVNADNETQLLNSEFKRRTMLDDVDEFRKVTA
                     FAVNSSNPDNIAVAVKHLVYETTDGGKTWNKCNGDLDPYNYITALCYDSLGTLYVGTS
                     FNGVYKREINKYIRFSKGLSLEPYSTGLVFYDPVARMREVGGRLYLSQYFNKGLYVLE
                     QTEWIKTDFDTLDKRPDNYIADFERPIVEDDSVVNNLPIVYSENSRKKNMEISGFDND
                     HVKKESYFGGVLYHADDGFSLFVPDPELPVRSSLKDEAKGKKSLYTSTHYAVKGVDRL
                     ISIIKKSGLNALVIDVKDDYGDIYIPVAGKDFVKAGAIKTSIDLKPVLEKLKKENIYA
                     IARIVVFKDKRLFDAYDNRYTIRDKATGKPWRYGRERWVDPFSQFVRDYNINVALAAQ
                     NFGFDEVQFDYIRFPADGPVERCDYTFARYKDAFKSEAMEDFLQQASSKITVPVSTDI
                     YGITASYNFGNRIGQDIEVYAKHMDAVSSMVYPSHYGSRYMMQGSREERPYRIVYDSG
                     VRSLFLSRVKVYMRPYLQAFVMASPTFGTGYIQNQVQGVYDSGCDGYIFWHAGGKYDV
                     VRRALSK"
     gene            complement(7236..8915)
                     /locus_tag="P5123_05180"
     CDS             complement(7236..8915)
                     /locus_tag="P5123_05180"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014803184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HRX15682.1"
                     /translation="MHKPAIVQSDNTILLEVNNSGFEQARDAISPFAELEKSPEHIHT
                     YRITALSLWNAAASGLVLETIVDTLRTYSRYDIPETVFSNIAEQMQRYGLIRLVREED
                     RLLLECEEPFFLEEICRDKSVEIYIKEKLPDGRIEIKSNLRGHIKQALIKIGFPVQDL
                     AGYEPGDPMPLNMREESVSGKPFGLRDYQQSAVNAFYHGGGPEGGSGVVVLPCGAGKT
                     IVGIGTMACLQTQTLILVTNTVALRQWKNELIDKTDIDPENIGEFSGDSKKIKPVTIA
                     TYNILTYRKNKNSDFLHFSLFGEKNWGLIIYDEVHLLPAPVFRMTSEIQSKRRLGLTA
                     TLIREDGMEKDVFSLIGPKKYDIPWKVLEKSSWIAEAICTEIRVTLPDEIRYHYSVAK
                     EREKFRIASENEVKNDIISTLLKYHNGASVLIIGQYISQLKQISTRFKLPMITGSTPT
                     VEREKLYDLFKTGEIKTLIVSKVANFSIDLPDANVAIQVSGTFGSRQEEAQRLGRILR
                     PKEDQNKAYFYTVITNHTAEEKFAHNRQLFLTEQGYTYRILNREMFDEEYC"
     gene            9261..9848
                     /locus_tag="P5123_05185"
     CDS             9261..9848
                     /locus_tag="P5123_05185"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRX15683.1"
                     /translation="MHTKYKIIYLHKKILFPHGTLQIHAHSSKGSVLKPGETVLVVTT
                     RNILDPVFVRKRYITVAKVTHEINSAGLPMVEIKGMSRAYIESRENLHMGTYRKADSS
                     PLHNAEYTADLIRKKAQEFVFLIDIPESERLIYLMTFITQADELIDFISHYFITSFKI
                     KWRIYNHYSRQKRAELLISTLDKLINDVKKKLEER"
     gene            9850..10308
                     /locus_tag="P5123_05190"
     CDS             9850..10308
                     /locus_tag="P5123_05190"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRX15684.1"
                     /translation="MEKKIKGADAYYAIGWSKPFEYNRITASRILPDLPGILFFSDNS
                     KGAMKPLLAFATWREGLRTGMRDLFDDMFSKCKPIMPYAEKKGLIYRYTVIDSTKLDL
                     RDVFYWVLRSYNPVLNTIKGVTPTSRYGNIYVIERENAGNADNIRLPGSF"
     gene            10656..12530
                     /locus_tag="P5123_05195"
     CDS             10656..12530
                     /locus_tag="P5123_05195"
                     /inference="COORDINATES: protein motif:HMM:NF012437.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate/guanylate cyclase domain-containing
                     protein"
                     /protein_id="HRX15685.1"
                     /translation="MEKQKRVIFSVGLKLIFIISLIFTLSFAIMIYLATTLFKEDMTA
                     FIENNNHKISIASSHSTEIYIKNIIEKGYIAAENLIASKKTKIESNGENPLFLSKNDD
                     IIYMALVEKDDRTGLKNSHTFISTDFFGESGIDSEKAESFSGTISSQIEFAFNLNINI
                     FNPSPEFGYPLTGLAIPYRVIDDKTAQSVLFMLIKTDTLQNIVQMNDITTTYIVSDKG
                     IVLIHPEMPEVLAASDISALSIYEDFHKNPLDNGQKTYEENGISKIGSYQRINFSGFA
                     LGVITTVNQNRAFEAVIRLQRRNIMTAIIIITAVIMFIYFYAKTITSPVKNLVRATKR
                     VEKGDYSVSLKPLFNDEIGMLANAFNHMSQGLEERERIKDAFGKFVNPEIAERVTKEQ
                     LTLGGEMKNAAVFFSDIRSFTAISEKLHPEKVVEFLNEYMTLMVDCIDKTHGVVDKFI
                     GDAIMALWGTPISRGNDTENAVNAALMMRLYLKEFNKTRGSDEKPVISIGCGINTGPL
                     IAGQIGSANRMEYTVIGDTVNLASRIEALNKPFKTDILISEASYNMVKDIFIFEPMKK
                     ITVKGKTQPQQIYAVINRKDATEGPRTLTEVRQMLGFEDIDINQVNSEGNEEKFKILD
                     "
     gene            12502..>14013
                     /locus_tag="P5123_05200"
     CDS             12502..>14013
                     /locus_tag="P5123_05200"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRX15686.1"
                     /translation="MKKNSKYLISDLIFTFACLLIMSGTSYALYQDINERIDFSKNQQ
                     VGHIVFKRNSAQRKYASYSVWEHLNQKSPLYNKDTIRTSENSDVRLVLEGGIEVALEQ
                     QSLIFVMMTPRGFEIDFQGGEIHSIAVNRMTVKYRNSTITLEKGTNFSLCELDNNSVE
                     LTVNKGSAHVENSDAGIIKNVTDNESATITSNNVQITALQFRDCFPPNNTILVSPDDK
                     MPVNLTWKTEVSGTKVIVARNVQFSEIVASKENAENLNIDLSPGLYYWKLISNSTESR
                     TFSFEILKEKTPLIISPANNSNLIKSDKGFFEISWLTRGRADSYQVNIYKDGSDSPIE
                     SALTKNNSTMLDMPAEGTYQLVVTPIYHFGAAKKDMNSEAITVYVNSSAEKIPVKVLT
                     PSAQTAISLEAALHNGILCSWNSTRGKCRVELFSDNGSGHASGDPIFSEETEKNSIRL
                     TGNFKTGSYIIRVTPLSNSYSEGETHFQLNEGVGLTLISPTINSHFKAGSDITF"
BASE COUNT         4563 a         3007 c         2513 g         3930 t
ORIGIN      
        1 tgcagaataa tagaatctcc aggactataa gatcgccgtg ccggcaaaga tccgcttccg
       61 ccattagcgt tgtacccaag agtaaatccc gttggcggaa caatcggctc accagactcg
      121 ttacccgatc cgtccggctt tgccggatta ttaggcggat tatacggctc gatatctgga
      181 gattcagttc ggctaacgac tatacgatac tccttacttt tgccagctct gctttttaca
      241 acaatacgaa ccggatcact aaaatcaaga ctcgtctcac ctgatacagc atccaccctc
      301 tcgccgtcag aagcataaaa taacacattt tctcctgaaa catcaaacac aggcacaagc
      361 gcacgcaaat cagcgttata tggaagttgc acaaaaacat catttatatt atcagacata
      421 tcatgatcga tcaaacaagc ctcaatttcg ggataatttt taaaagacaa tatctttaat
      481 cgcaatgccg aaactctgta atcgagataa ctctcgttac gattatcata aacacgatac
      541 acacagtagt ccgaaaaatc acgaggagca gtcacgttag cgtgctcttt accgttttta
      601 tccacaaaaa agacttttac atcagagggt gatgtagtaa aagtaggaac caacccgtca
      661 agggatacag tatgaggcag atcaaaccag atagcattgc cttcgatatc atcttcgggg
      721 accataatat caccaatatt atagaaggta ctactcaaga tagcattatc acttgcagca
      781 aagccaaaac tttcgaacca tgcggcagcg ttttgcgccg taacggtgta gtcggtactt
      841 tttccatcat aagaatagag cttataggta tagctctgtt tatcagtaaa atcgagaccg
      901 tccaaaccgc ttgactgacg tttaccgtca acatacaacg cgtaaccatc aatctcaaac
      961 gaaggaacaa gccgcatacc ggtaccactc ggtggcaatg tatccttggg aaaaaccgcg
     1021 acaaaactca gtgaagattt atcataaacc gcatcgatat caaaaccgtc aagcccgttg
     1081 tctgctgccg cgaaagagaa acttttgacc gaccttgcgg ataaaaaatc aaaataatcg
     1141 gtgcgactac acgaaaaaaa agatagcaag ataataaaca aacatacaac tttccgattc
     1201 attattcacc ttctaaatgg ataatcggaa aatatttaaa acaattaact ttataatgtc
     1261 aattaaaatt taattagttt tattcaataa atatctggtg gtttaatcaa ttgtgctgca
     1321 accgtattca catgtcgtta caaaaaaatc gttaattgat ttattaatgg ggacggtagt
     1381 cacttaaact taatctaaag ttttatcagg acttctacca aacagaagct gatatacaaa
     1441 aacagcaccg aatataattg gaaccacaca aaatattatt ccaataagat attctccaga
     1501 cccaagaagc ccgcaaccaa ttgccgcaaa aacagaaaga acaaaagtga caaacccagt
     1561 caaaattacg aattgagtaa ttattttttt atccttagct ttaagtccct cacgaagcgc
     1621 ttctggagta aacgaaaact caaatcctgc ctctttacca cgggttttcg aataatttaa
     1681 tgatttgcct cctcccatcg aaccaggcag agttcttccc gaagtgtcct gaaacctgat
     1741 accggcatta cgagcgtaat taattgaaaa gaagacagcg aaggaaagag caaaagcggc
     1801 taacgtaata aaaaaaaaca taaaaaccgt tgcaatcata acattctcca tcttaaacgt
     1861 tattgcttta agcagcattc aaattattgg taacagatat actaaatgta cttcatattt
     1921 atggattagc aacaaaaaac gctgttgggt gattattgtt atgcaataat tcaaaaacag
     1981 taaccctaat accttcttaa aagttccaaa aaggctctgt caagtttacg tccatgccaa
     2041 tcctcatatt gcacatctgc acgacccgaa tcaagaatgc caaactcgcc cgaaaactcc
     2101 cataatgaat agccaatatc atgttttgtt aatattttaa gaacatcttc aaaccaggat
     2161 aaaacaacgt catgcggtgt gtatatatag gctccgccct ctccacagtg tactgcaaca
     2221 ccgttttttt taagatcggc ccatccctta taatgctctt cgagacgatc ttgattaaaa
     2281 ggttttccat tttcaataat gcccggccaa accggttttt gccactcgta atcctgtacc
     2341 cactcagcct tatagtggct aataccaaaa ggcgcgtagg cccgacagct ctgcactgcc
     2401 cctgtatctg taagttctaa tacgggctct gttcctacgc ttagcccctc caccattatt
     2461 atccgatcag gatcaacctt tcgaatattt tcaatagccc ctttcataac acgcacataa
     2521 tcctctcggc acattttacc atcagctgga tttggaggtt cgtttaaaag atcaaagctt
     2581 agcatatcac cagatattga cttataacgt ttagcaaaag tttcccaata atggttaaaa
     2641 agtgcaaccg gcagctcatc tttaaaaaga tcaaagggtt ccaaaatttc ggtattaata
     2701 caatatccgg gtgctctatg aatatttagc gaaacatgca gaccgttagc attgccaagt
     2761 tcgataacgc ggtctatttt ttttaactct atctcatcca taacaaacat atctttagaa
     2821 tcagaaagcc aattacggta acacatgggg attcgaataa agttaaaacc ccagtccgcg
     2881 atccacttta tctcgtcggc agagaagttt ccgtccgaac gataatactt tccgttcatc
     2941 ttggactctt cttcagatat aaaatagttt aaaaggttaa atccgcgcca tctcggtatt
     3001 gtctttgcag cagtcataag tttctccata gaagaatatc aaacaatcat tatatgcaag
     3061 atcaatttat aaacgcaagg agttttatag taacaaataa aaagaaaact ggtaatggga
     3121 aataaatgac attctcccac catattgacg atactgtatg atctcgcata ttaattacgt
     3181 taataaaaaa cccattaatg ctaaaaaaca accctttata agtaataaac ataagaatcg
     3241 aagacaaaat agccaacatt tacaatagtc attatacaat ttcttcgttg ggtcaattcc
     3301 attgcacgac tggggcgtct tggcaacact atagacaagg tagaagaggc cactctgtct
     3361 gctttacagc gccgtcttaa taatcaggca tttggatgtg tgcctgttag cacggtctcc
     3421 acaaaaggat atgccataaa aactgactca attggatacc ttcagtacat tgacacttct
     3481 tcgttgcaaa tatgttgtga agaattatcc gcctatctta acattgcagt tcaaccaggc
     3541 acttttatag gccccggcca tattttagct tatctggata cagaaaatag tgataacaat
     3601 gattttgact acacacatat ccacaaagca ttcatgattg ggaaacagcg cgtctttgat
     3661 gatgaccctc gatatggtct tatagtactt agccaaattg ccgacagagc ccatttctcc
     3721 aggagtaaac gatccaggca cagctattga catcataagc acttttgtac ggctcttctc
     3781 aatatggatt aactttaaaa acgactctca agaatcagaa gccaaatttg ataaagtacg
     3841 gttttgcaca ctgtcggtta aagatatgtt tgatgacgca ttcactgcca tagaacgtga
     3901 tggcagcagt cttatagaag ttacaattaa gttgataaaa tcactttcgg ctctttcttc
     3961 aatcggagat gagcaagtac agcaagcagc agcctatcat gctaaacgga tcataaaata
     4021 cgcagagcaa aaacttgttc tagaaagtga catcaataac gttcgcgacg aggctcaaat
     4081 tattttttca gttaaaccat aaaaatgcaa ttttatattt ttaagtaccg catgataaaa
     4141 catttgcatt attatcaaaa ttatgtattt aattctgtta ctacccgcat ggaggtttat
     4201 aaatgatcta tcgtgaatat ggctcaaccg gcaagatggt ttcggcactt ggtttcggag
     4261 gaatgcgttt taaagatatc aaagacatcg acacctgtgc atcaatgatg ctgactgccg
     4321 cccgtggcgg aataaattac tttgacactg ctcctaagta cttcggtaca ggaagtgaag
     4381 aagctttcgc gaagggtctg gccgcaatga gaaacgaaaa ccttccctat tatctttcta
     4441 caaaaaccga cgagtctcac cctgactcca tccgccgtga gattgaagcg caactgaaac
     4501 ggcttgatgt taatcatatc gatttttacc atatctggtg cataaccagt cttgataatt
     4561 ggcgcgaacg caaaaaaaat ggagttcttg aaacatttat taaacttaaa gaggagggtt
     4621 taataaaaca tatatgtgta tcaagccatc ttattggcga tgagattaaa gagctcttaa
     4681 tggaagaaat tttcgaagga gtcttgttcg gctactcagc ttacaatttc aaaacacgtc
     4741 agtcagcctt tgacgcgata acagaaagaa gaattggctg tgttgtaatg aacccgcttg
     4801 gcggaggcat tattcctgga aacccggaaa ttttccgttt tttacaaaca gtaccagacg
     4861 aaacagtcgt acaggccgca ctacgatttc tatttgcgca tcaagaaata accatttctt
     4921 tggtaggctt cgaaactgaa gagcaggtta acgaagccct tgccgccgcc aacagccctt
     4981 tgcagcttag ccctgacaag cttaaacaga ttaagaagaa cgctacggta tcactagagg
     5041 gaatttgtac tggctgtcaa tactgcgatc actgcccagt taatattcca attccaaaat
     5101 atatggatgc atataatcaa aaaatattaa ccgattcaaa aaccgctatt accgatcgtc
     5161 ttttttggca ttggagcctt aaaccagaag atgccgcagc ctgtatagag tgcggtcgtt
     5221 gtgaagaaga gtgcacacaa catctgaaca ttattgaacg tctaaaagag atatctgtat
     5281 tacccgctaa ataaccttaa aactgcttat tatgtttaac gcgggtattt taagcccgcg
     5341 ttaaaaacaa actaccttat tttgataaag ctcggcgcac aacatcgtat ttaccgccgg
     5401 catgccaaaa aatataaccg tcgcatccgc tatcataaac accttgaacc tggttttgta
     5461 tatatcccgt gccaaacgtt ggtgacgcca taacaaatgc ctgcaaataa ggacgcatat
     5521 aaacctttac cctagaaaga aaaagcgacc tgacacccga atcataaaca attctgtaag
     5581 gtctttcttc ccgcgatcct tgcatcatat aacgggaccc gtaatgtgaa gggtacacca
     5641 tcgacgaaac ggcatccata tgctttgcat aaacttcgat atcctggcca atacggttgc
     5701 caaagttata cgatgcggtt attccataaa tatctgttga aacaggaaca gtaatttttg
     5761 atgaagcctg ctgaagaaaa tcttccattg cttcggactt aaaagcatct ttataacgcg
     5821 cgaatgtgta atcacaacgc tcaacaggtc catcggccgg aaacctgata taatcgaatt
     5881 gcacctcatc aaatccgaaa ttttgcgcgg caagagctac attgatatta taatctctca
     5941 caaattgcga aaaagggtca acccaccgtt cacgaccata acgccacggc tttcccgtag
     6001 ctttatctcg tatggtatag cgattatcgt atgcgtcaaa aagacgttta tccttaaaaa
     6061 ccactatgcg tgctatagca tatatatttt ctttttttaa cttctctaaa acaggcttta
     6121 aatcaatact ggtcttaata gcacctgctt taacgaaatc tttaccagca acaggaatat
     6181 agatatcgcc atagtcatct tttacatcga taacaagcgc attcaacccg gactttttta
     6241 ttatagagat taagcggtct acccctttta cggcatagtg tgtgcttgta taaagagatt
     6301 ttttgccttt agcctcatcc tttaatgacg acctaacggg taattccgga tcaggaacaa
     6361 aaagagaaaa cccatcgtct gcatggtaaa gaactccacc gaaatagctc tcttttttta
     6421 catgatcatt gtcaaaacct gaaatttcca tgtttttctt tcgactattt tcactataaa
     6481 caattggtaa attatttact acggaatcat cttctacaat aggtctttca aaatcggcaa
     6541 tataattatc cgggcgcttg tccaacgtat caaagtcggt ttttatccat tctgtttgtt
     6601 caagaacgta gagccccttg ttaaagtatt gagaaagata gagtcgccct cccacttcgc
     6661 gcatacgcgc gacaggatca taaaaaacca gacccgtact atagggttca agcgatagtc
     6721 ccttggaaaa tctgatatac ttatttatct cgcgtttgta tacaccatta aaagaagttc
     6781 ccacatacaa tgttcccaat gaatcataac aaagcgctgt aatgtagtta tatgggtcaa
     6841 gatctccgtt gcatttattc caggttttgc caccgtctgt tgtttcataa acaagatgtt
     6901 ttacagcaac cgcaatatta tcaggattgg aactattaac cgcaaacgct gtcactttgc
     6961 gaaactcatc gacatcatca agcatggttc gccttttaaa ttcactattc agaagttgtg
     7021 tttcattgtc agcatttaca gatttaacaa ataaaccctg atcatatgta gcaatataaa
     7081 tattatcttt aacacgctga gcgaaatgaa cacgataagc gggacgcttt tgaacagctg
     7141 cgtactcttt attagaacag ttacttaata aaataaatgt aaatgaaaga aacaggataa
     7201 atataaccca agaggaaaaa aatgcggagc ttcgcttagc aatactcttc atcaaacatc
     7261 tcacgattga gtatacggta ggtgtaccct tgctcggtaa gaaaaagctg gcgattatgg
     7321 gcaaacttct cttcggcagt atgatttgta attactgtat aaaaataggc tttgttttga
     7381 tcttctttag gtcgcaagat acggccaaga cgctgcgcct cctcctgtct tgaaccaaaa
     7441 gtaccagaaa cctgtattgc aacattggca tcgggaagat caatcgagaa attagcaacc
     7501 tttgatacta ttaacgtttt aatctcaccc gttttaaaaa gatcatacag cttctctcgt
     7561 tcaacggtcg gtgtacttcc agttatcatt ggaagtttaa atctcgtaga aatctgtttt
     7621 aactgagata tatactgacc aattatcaaa acggaagcac cattatgata ctttaataaa
     7681 gtacttatta tatcgttttt tacctcgttt tcagaagcaa tacggaattt ctcgcgttcc
     7741 tttgcaaccg aatagtgata acgtatttca tccggtagag ttacccttat ttcggtacaa
     7801 atcgcttcgg ctatccagct ggacttttcc agaaccttcc agggaatatc atattttttc
     7861 gggccgatta aagaaaagac atccttttcc ataccgtctt cgcgtatcag tgtagcagta
     7921 agccctaacc tgcgcttgga ttgtatttca gaagtcattc gaaaaacagg agcaggaaga
     7981 aggtgaactt catcatagat tatcagaccc cagttttttt cgccaaaaag cgaaaaatgc
     8041 aagaaatcag aatttttgtt tttacggtat gtcaatatat tataagtagc tatagttacc
     8101 ggttttatct ttttcgagtc gcctgaaaac tcaccaatat tttcgggatc aatatcagtt
     8161 ttatcgataa gctcgttttt ccattggcgc agagctactg tatttgttac aagtattaac
     8221 gtctgagtct gcaaacaagc catggttcct ataccgacaa ttgttttacc cgcaccgcaa
     8281 ggcaaaacga caacaccgct tccaccttca ggaccaccgc catggtaaaa agcgtttacc
     8341 gcgctctgct gataatcacg caggccgaaa ggttttccag aaaccgactc ttcccgcata
     8401 ttaaggggca tcgggtcacc cggctcataa cctgctaaat cttgaactgg aaaaccgatt
     8461 tttatcaaag cctgctttat atgtccacga aggttgctct taatttctat acggccgtca
     8521 ggaagttttt cttttatgta aatctcgaca ctcttgtcac ggcaaatctc ttcaagaaaa
     8581 aagggctctt cacattcaag aagcaacctg tcttcttcac gtaccagacg tatcagtccg
     8641 taacgctgca tctgctcggc tatgttagaa aaaaccgttt cgggaatatc ataacggcta
     8701 taagttcgta atgtatcaac aattgtctca agcaccaaac ctgaggctgc agcattccaa
     8761 agggaaagag ccgttatacg atatgtgtgt atatgctccg ggcttttttc cagttcggca
     8821 aaaggagata tcgcatccct ggcctgttca aaaccggagt tgttaacttc aagcagtatt
     8881 gtgttgtcgc tctggacaat cgcgggttta tgcatcaaat attcctgatt acttcaattt
     8941 atgcaataaa gcataaaggc ttcaattttg tcaactgcta ttttgtacac cttgcccttt
     9001 atagctcata attggaaaga ttattgccaa taaagggaac tttctcggtt aaaaacttaa
     9061 caacaaaact aacttgcaat aagaccggca ttgtaataat ttgtcagaag aatgtattgg
     9121 taatctctaa caattaaatt tgtaacaagg aacttgcgaa aaattcctat atatatgggc
     9181 tttaaacagc atagccgttc cggaaaaggt aatttttcga agttccctat tatcagaacc
     9241 ctagcggcaa ccggtaaaaa atgcatacaa aatacaaaat tatatatctg cataaaaaaa
     9301 tcctgtttcc ccatggaaca cttcaaatac acgcccactc ttcaaagggc tctgttttaa
     9361 aaccgggcga aacagtcttg gtcgttacta ctcgtaatat tctggatccg gttttcgtac
     9421 gaaaacgcta tataacagtc gccaaggtta ctcatgagat aaactcggca ggtttaccca
     9481 tggtagagat caaagggatg agccgcgcgt acattgaaag ccgtgaaaat ctgcacatgg
     9541 gaacatatcg caaggccgac tcttcccctt tacacaatgc cgaatatacc gccgatctta
     9601 tacgcaaaaa agcacaagaa tttgtttttc ttattgatat ccccgaatcc gagcgattga
     9661 tataccttat gacatttata acccaagctg atgagctgat tgactttata tcacactact
     9721 ttatcacctc atttaaaata aaatggcgta tttacaacca ctacagtcga caaaaacggg
     9781 cggaacttct aatttctact cttgacaaat tgataaacga tgtgaaaaaa aaactggaag
     9841 agaggtaaaa tggaaaaaaa aatcaaagga gcggacgcct attacgctat tggatggtca
     9901 aagccattcg aatataatcg tattacagct tcacgaatac ttccagatct tcccggaata
     9961 cttttcttct cagataacag caagggagca atgaaacctc tccttgcctt cgccacatgg
    10021 cgtgaaggtc tgcgtactgg aatgagagat ctttttgacg acatgttctc caaatgcaag
    10081 ccgattatgc cctacgccga gaagaaaggg cttatatacc gctacacggt aatcgactca
    10141 acaaagctcg atcttcgaga tgttttttac tgggttcttc gaagctacaa ccctgtttta
    10201 aatacaatta aaggtgttac ccccacatcg agatacggca atatctatgt aatagagcgt
    10261 gaaaacgcag gtaatgccga caacattcgc cttcccggaa gtttttaacg gctaacttta
    10321 taaccagact taacagttct gacacattac aattatagaa gttcgtattt aaaaaaatta
    10381 ccaaatacgt ttatctttcc gtaaaaacca tatatttttc ttgctttttc tgtatagtga
    10441 tatttgaata atacagttgg tttgcggaca ttatctgttt gtaaatatcc acaaatcacg
    10501 tatagcttct atttcaaaga atcagatttt ggcggccact attaattgtt tataccgtct
    10561 accggttaaa tatattttat ataatagcaa ttatctggaa acagactaat atattatcgc
    10621 gattttatcg cgattttctc atggagaata gtttaatgga aaaacagaaa agagtaatct
    10681 tctccgtagg cttaaagctg atatttatta tttcactcat tttcaccctc tctttcgcaa
    10741 tcatgatata ccttgcgaca acccttttca aagaagacat gactgctttc atcgaaaaca
    10801 ataatcacaa aatatccatt gcctcttcac atagtaccga aatatatatt aaaaacataa
    10861 tcgaaaaagg ctatattgct gcggaaaacc ttattgcttc taaaaaaact aaaattgaat
    10921 ccaacggcga aaacccgctt tttttatcta aaaacgatga cattatttat atggctcttg
    10981 tagagaaaga tgaccgtaca ggtcttaaaa acagccacac ttttatcagc accgattttt
    11041 ttggcgaaag cgggatagat agtgaaaaag ccgaatcctt ttccggaaca atctcctctc
    11101 aaattgaatt tgcctttaat cttaatatca acatatttaa tccttcaccc gagttcggtt
    11161 acccccttac agggcttgca atcccttatc gggtaattga tgacaagacc gcacaaagcg
    11221 tactctttat gcttattaaa accgacacat tgcaaaatat cgttcaaatg aacgatatta
    11281 ccacaaccta tattgttagt gacaaaggaa ttgtgcttat tcaccctgaa atgcccgagg
    11341 ttcttgcggc atcagacatc tccgctctat ctatttatga ggattttcat aaaaatccac
    11401 tggataatgg ccaaaaaact tatgaagaaa acggaatatc aaaaattgga tcgtatcaac
    11461 gaatcaattt ttcaggtttt gcgctgggtg taatcaccac tgtaaatcaa aacagagcct
    11521 ttgaggccgt cattagactt cagcgccgca atattatgac agccattatc attataactg
    11581 ctgtaataat gtttatctat ttttacgcga aaacgattac cagcccggta aagaaccttg
    11641 ttcgggcaac aaaacgggtc gaaaaaggtg attactcagt ttcactaaaa cctcttttta
    11701 atgacgaaat cggaatgctg gctaatgcat ttaaccatat gagtcaggga cttgaagaac
    11761 gggaacgaat aaaggatgct tttggaaaat ttgtaaaccc ggaaattgcc gagcgcgtaa
    11821 caaaagagca gctcactctt ggtggtgaaa tgaaaaacgc tgctgtattt ttttcagata
    11881 ttcgctcttt taccgccatt tcagaaaagt tacatccgga gaaggttgta gaatttctaa
    11941 acgaatatat gactcttatg gtcgactgta ttgacaagac tcacggtgtc gttgataaat
    12001 ttatcggcga tgcgattatg gcgctatggg gaactcccat atcccgtggc aatgatacag
    12061 aaaacgcggt taacgcagca ttgatgatgc gcctgtactt aaaggagttt aacaagacgc
    12121 gaggcagcga cgagaagcct gtcatcagta taggctgcgg aatcaataca ggtcctctta
    12181 ttgcggggca gatcggctcg gccaatagaa tggagtacac ggtcataggt gatacagtta
    12241 accttgcctc ccgtatcgag gctcttaata agccatttaa aacagatatt cttataagtg
    12301 aagcttctta caatatggtt aaggatatat ttatattcga acctatgaag aaaataactg
    12361 taaaaggcaa aacccaaccg cagcaaatat acgctgtaat taaccgcaaa gacgctacag
    12421 aaggaccgcg cactttaaca gaagttaggc aaatgttagg gtttgaagat atcgatataa
    12481 atcaggtgaa tagtgaaggc aatgaagaaa aattcaaaat acttgattag tgatttaata
    12541 tttacctttg catgcttgct tatcatgtct ggtacgtcat acgcgcttta tcaggatata
    12601 aacgaacgca ttgatttctc taaaaatcag caggttggtc acatagtatt caagagaaac
    12661 agtgcacaac gaaaatatgc cagctattct gtgtgggaac atcttaatca aaagagtcct
    12721 ctttacaata aggacacaat acgtacatcc gaaaactcgg acgtcagact tgtactcgaa
    12781 ggcggtatcg aagtagcctt ggaacaacaa tcactgattt tcgttatgat gactccacga
    12841 gggttcgaaa tagatttcca aggtggcgag atccactcca tagccgttaa cagaatgacc
    12901 gtcaaatacc gcaacagcac cattaccctt gaaaaaggca ccaatttttc actttgtgaa
    12961 ctggataaca attcagtaga actcaccgta aacaagggta gcgcccatgt cgaaaattct
    13021 gacgccggta taatcaagaa tgtcacagat aacgaatcgg caacaataac atcaaataat
    13081 gtgcaaatca cggcactaca attcagagac tgttttcccc cgaacaatac aattcttgta
    13141 agccccgatg acaaaatgcc agttaatttg acctggaaaa cagaagtttc aggtactaaa
    13201 gtaatagttg cccgcaacgt acaatttagt gaaattgttg cgtcaaaaga aaatgctgaa
    13261 aacttaaata tagatctttc cccaggactg tactactgga agctaatatc caatagtact
    13321 gaaagcagaa ccttctcttt tgagatatta aaagagaaga ctccgctaat aataagccct
    13381 gccaacaaca gtaatcttat taagtccgat aaaggctttt tcgagatatc atggcttaca
    13441 agaggtcggg cagactcgta tcaagtaaac atctataaag acggttcaga ctccccgata
    13501 gaaagtgcac ttacaaaaaa caactccact atgctcgaca tgcctgccga aggtacatat
    13561 cagttggtag tgacccccat ataccacttt ggagcagcca aaaaagatat gaactctgag
    13621 gcaattacgg tttacgttaa ctcttctgcc gaaaaaatac ctgtaaaggt acttactcca
    13681 tccgctcaaa cggcaatatc acttgaggcg gcacttcata acggaatttt atgctcttgg
    13741 aatagtaccc gcggcaaatg cagagttgaa ctattttctg ataacggatc aggccatgcc
    13801 tcaggagatc caatattttc ggaagagacc gaaaaaaaca gtataaggtt aacaggtaat
    13861 tttaaaactg gaagctatat aattagagtc acacctctat ctaatagcta tagcgaaggg
    13921 gagacacatt ttcaactaaa cgagggagtg ggacttactc ttatttcccc aaccataaat
    13981 tctcacttca aagccggctc tgacataaca ttt
//
LOCUS       DAOTGZ010000053        14002 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_3064
            NODE_5232_length_14002_cov_4.930451, whole genome shotgun sequence.
ACCESSION   DAOTGZ010000053 DAOTGZ010000000
VERSION     DAOTGZ010000053.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868088
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 14002)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 14002)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:22:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,785
            CDSs (total)                      :: 2,751
            Genes (coding)                    :: 2,732
            CDSs (with protein)               :: 2,732
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 12 of 19
            Pseudo Genes (internal stop)      :: 5 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14002
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5232_length_14002_cov_4.930451"
                     /isolate="AMR_MDS_3064"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..587)
                     /locus_tag="P5123_05205"
     CDS             complement(<1..587)
                     /locus_tag="P5123_05205"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRX15687.1"
                     /translation="MSAKRCVDEKISEIYDRYASGNTIDCLKSLSSSELAEFQKLEKT
                     LSLVHGLRAVHILNRDYFTTKTLNCCKQTSLRYAGFGKYIASKPFVSGAVAAVFAVVL
                     TVSLVTLNRENDSVPFSGTTIANEFNINSEYEKDINVIKNAVISLGGRVVDSNDGILK
                     VQASISDYMAIKKELTLSAEEKVFLNNNNRLRSVGT"
     gene            complement(584..1159)
                     /locus_tag="P5123_05210"
     CDS             complement(584..1159)
                     /locus_tag="P5123_05210"
                     /inference="COORDINATES: protein motif:HMM:NF019265.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor"
                     /protein_id="HRX15688.1"
                     /translation="MSDLTEEEIIDRVKNGETELLGVLIEKYQDRVFRYTYSNLGNYD
                     DALEATQDIFVSVFRSIAKFRGESKFSTWLYSITSNYCKNYKRKRGRMSIVSLFRSDD
                     EDFGELPIEDEREDLEKQFELSNAMDIAREELAKLPDDYKNILLLREIEEMSYEDISE
                     ELNISLSNVKVRIHRGRAMLKQRLIERGVVE"
     gene            complement(1185..2399)
                     /gene="tyrS"
                     /locus_tag="P5123_05215"
     CDS             complement(1185..2399)
                     /gene="tyrS"
                     /locus_tag="P5123_05215"
                     /EC_number="6.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013120723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004831 - tyrosine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006437 - tyrosyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine--tRNA ligase"
                     /protein_id="HRX15689.1"
                     /translation="MIKNDITLLKRGTEEILPEDDFDKKVKESEEKQKPLIIKAGFDP
                     TAPDIHLGHTVLIRKMKHFQQMGHTVVFLIGDFTGMIGDPTGKSETRKKLSREEVLEN
                     AETYKKQVFKILDPDKTIIEFNSKWCSKMDFANVLELTSHYNVARMLERDDFSKRYKT
                     GKSISIIEFMYPLVQGYDSVMLKADVELGGTDQKFNLLVGRDLQKEYGCSQQSIMTVP
                     LLVGLDGVQKMSKSLGNYIGIDEKPADIFGKVMSISDDLMFTYFNLVTDVPYEEIDDI
                     KMKMQDGAVHPRDVKVRLAKDICAQFYDEAIAEEAEQQFNTVFVKKDVPDDIEEYKIE
                     SDILKEGKIWIIKLLVLAGLAKSNGEARRLIQGGGVYIDGETVLDADFELVLPSSSII
                     KVGKRRFIRIVC"
     gene            complement(2659..3165)
                     /gene="ispF"
                     /locus_tag="P5123_05220"
     CDS             complement(2659..3165)
                     /gene="ispF"
                     /locus_tag="P5123_05220"
                     /EC_number="4.6.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008705528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008685 - 2-C-methyl-D-erythritol
                     2,4-cyclodiphosphate synthase activity [Evidence IEA];
                     GO_process: GO:0019288 - isopentenyl diphosphate
                     biosynthetic process, methylerythritol 4-phosphate pathway
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-C-methyl-D-erythritol 2,4-cyclodiphosphate
                     synthase"
                     /protein_id="HRX15690.1"
                     /translation="MIRSLRCGTGFDVHRFCQNRDLVLGGVVVKHPLGLAGHSDADVL
                     VHALMDALLGAAALPDIGSLFPDNDVTYQNISSIVLLQKVKSILNESGFEIINIDTTI
                     ICEKPKVGPYVALMKKKIGKALEVSEDLVGIKATTTEKLGFTGREEGIACQAAVLLFK
                     KTENKTID"
     gene            complement(3162..3461)
                     /locus_tag="P5123_05225"
     CDS             complement(3162..3461)
                     /locus_tag="P5123_05225"
                     /inference="COORDINATES: protein motif:HMM:TIGR00377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043856 - anti-sigma factor antagonist
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="STAS domain-containing protein"
                     /protein_id="HRX15691.1"
                     /translation="MDIKITENESSVLVKVSGDIEMMTIKDFKDQLLLLGKDSDKDIE
                     LDMGDVNYIDSSGVGVLISLLKLQKKKDKSVTITEVSDKVLNVLKLSSLADVFDL"
     gene            complement(3541..5562)
                     /locus_tag="P5123_05230"
     CDS             complement(3541..5562)
                     /locus_tag="P5123_05230"
                     /inference="COORDINATES: protein motif:HMM:NF013528.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DHH family phosphoesterase"
                     /protein_id="HRX15692.1"
                     /translation="MKENWLLKPVDSEQIKYLAETSQLDHDLVAILMRRGYTDINSII
                     DLMYPSYAALHSPFLMLNMYQAVDLLRKSTVSGKKIGIFSDSDLDGLTSLYIVKNLLD
                     KLGIETFCYYPNREHSEYGLTDEVAEIFIEQKVDLLITLDCGIRDIIPISILRKSNVS
                     VLVCDHHEPDTVLPEAVIVNPKLHNCGYPFKELAGVGVAFKLCQAFLLSFLPVYGKRT
                     ALLFKKDDCYAMHFFMDGIALDDDKNEFFYFQEIHRYTGNEDIIFYYGIDEKEIVEIE
                     RYTNHSITNIVNQAFGANTFSALFKKLLLIYKNEKNQVEKALVHVYRESIFMIPAKIG
                     DFIREVMPYIAIGTIADVVPVRDENRIFTAKGIDQFNDCDSSTLVQLKNFCKTRITSK
                     VIGWQVAPLLNSPGRFGKTALTADFFLQPQNSVDILCTISELNDYRKKYVKDLVAGVI
                     SKNHEDFLDCSVIYTRFDKIEEGISGLIASRLVDEYDKPAVVVVQIDDHYCKGSGRSP
                     VSGFYSLFEPHSSIFERFGGHDQALGFTICNENLVKLKTLIEGFNFGENFGKKEIIID
                     LEIDVSNIDYSFIKKISSLEPFGAENQPPVFLSRGVAFEELKIFGKKQEHAKLFTTNK
                     LLEIIIWNGSQKAKELFEAGVADIVYSLELSNFMGSQIIRMNMIETKIN"
     gene            complement(5559..6209)
                     /locus_tag="P5123_05235"
     CDS             complement(5559..6209)
                     /locus_tag="P5123_05235"
                     /inference="COORDINATES: protein motif:HMM:NF024848.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004523 - RNA-DNA hybrid ribonuclease
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="reverse transcriptase-like protein"
                     /protein_id="HRX15693.1"
                     /translation="MMLSIDKVLSLLEDGKTVQKIAELADSSIEDVISLIREARKIIT
                     QKDPQKSRKKVKFKKKIEQNSDLGVIDNEELDIFKGADLSVVPLENILTFYCAVSESN
                     GAVSVAIIIQDNEGRRVGKVQYRENTKSKRLALLKSLLRCEQIASYFKSVKTRLRFDD
                     EYLYRQITGKNTEIEKKLINYVGDMRVLLESRKNEILIELIESHQNEKAHFLTENQ"
     gene            complement(6255..6956)
                     /locus_tag="P5123_05240"
     CDS             complement(6255..6956)
                     /locus_tag="P5123_05240"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRX15694.1"
                     /translation="MKENIRQMEELQRYTDAVIRETGEIILARKRIEEIQKNQVYTKQ
                     KFDKRLSELKKLKKETSSLELILEEADAAASRVKEKINGAQNEKELLAGSNELDKINS
                     EKDIIEEQLLANFDKISDEEELIKEEEISLEKNTNSDNATLKELNERIDRLQTIKEEN
                     QKKFEAGLLLLEPKIRSRFAKLSESKDGKAIVKMENGVCMGCNNTVPPNDLSVVNSGE
                     LCICTNCGKYLYSSR"
     gene            complement(6963..7754)
                     /locus_tag="P5123_05245"
     CDS             complement(6963..7754)
                     /locus_tag="P5123_05245"
                     /inference="COORDINATES: protein motif:HMM:NF013909.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0051540 - metal cluster binding [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Nif3-like dinuclear metal center hexameric
                     protein"
                     /protein_id="HRX15695.1"
                     /translation="MLVKDLIDYIRKKFPEDIQESYDNSGIQVYRADDKLTGVLLALD
                     IHEKVIDDALRFGCNLIITHHPLFFKSIKSVTTESFTGRIINYLIKNNISHYAIHTNL
                     DRVYWNKHAQMLGLLKIGVIDPYLNTGYGYGAYGEFPDELYMEDVLRLVKKTFGNEYL
                     IYTGELDKKIKSVAIMPGSGSSLVEKMTEEKVDLYMCGDIKYHDAFLAEVNGLNLIDI
                     SHFSSEIHYMTFLSVDLQDYLTKASVANGIKLFTLANNPLKCFQG"
     gene            complement(8161..9189)
                     /gene="queA"
                     /locus_tag="P5123_05250"
     CDS             complement(8161..9189)
                     /gene="queA"
                     /locus_tag="P5123_05250"
                     /EC_number="2.4.99.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012548608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA preQ1(34) S-adenosylmethionine
                     ribosyltransferase-isomerase QueA"
                     /protein_id="HRX15696.1"
                     /translation="MVQKLEDYNFYLPENLIAQNPAENREDSRLLVLSRSEKTINHDH
                     FRNIYQHINAGDVLVFNDAKVIPARLCFKRSSGAEVEIVLARMINRSEWYVISNRTKK
                     LKPGEKIQSLAEPGLNLEIVERKEDFLFVKSSSPLTDELLMRIGSIPLPPYIQREASA
                     DDIKRYQTIYARKTGAVAAPTAGLHFTSEIMDKLEKKGVILKYLTLYVSWGTFSPVRS
                     DNISEHKMHSEYYDLSKDVTTEINKARLEKRRVIAVGTTALRVLESTFVNGVNVPGSG
                     DTDIFIYPPYKIKSIDALITNFHTPKSTLLMLVSAFAGYDAIMNAYKEAVDNNYRFFS
                     YGDSMLIC"
     gene            complement(9201..10325)
                     /locus_tag="P5123_05255"
     CDS             complement(9201..10325)
                     /locus_tag="P5123_05255"
                     /inference="COORDINATES: protein motif:HMM:TIGR02669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SpoIID/LytB domain-containing protein"
                     /protein_id="HRX15697.1"
                     /translation="MSCKFIITITIFIFITSCTPSQQFLEQRGEVISDKSSFIRVLLH
                     KSDAPVVVGSVKRIKVKNLKSGKLLVDGKSKKIKFFSDKLSSPVEIESWDAPLIVNGK
                     PYRGKIELHNVTGKIYVINVVSMEHYIMGVVSAEMSPNWHIEAVKAQAVAARTYAFHT
                     LLNKKSASIYDLDSTTKFQVYKGMEGETTNSNLAVSQTAGQILSYNNKPILALFHSTC
                     GGRIINNKYVWDGEDLPYLSDKVVPYGKSSPHYKWKTELTLYEIKKVLDSRYNNIGAV
                     TGLSFKKHQGRVVEVIITHKNGRANVSGNDFRMMIPGGKVKSQFFNAFRTKNGLRLEG
                     RGWGHGVGMCQYSAKEMAEEGKTYKTILSFFYKDVQFTKF"
     gene            complement(10332..11243)
                     /gene="xerD"
                     /locus_tag="P5123_05260"
     CDS             complement(10332..11243)
                     /gene="xerD"
                     /locus_tag="P5123_05260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015678684.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009009 - site-specific recombinase
                     activity [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific tyrosine recombinase XerD"
                     /protein_id="HRX15698.1"
                     /translation="MARSITLEENKEILKFKRFLKVEKGLSQNSIYSYSYDLKKFNEF
                     LHSANKSILSADSEDVKRFLSFQQQKKNNSSRTLARSLAAIRQFYNFISSTDLDVSNP
                     TIKIETPQIRKSLPDFLSVEEVDALFNSISESDCYELRDKTIFELLYSCGLRISEAIE
                     LKFFDIDFEDKLIKVTGKGNKERFVPVGARAVRLMKLYLERSREIILGSRESEYLFIS
                     KKGSMLNRKSVWRLLKGYVSRTNIKKNITPHTLRHSFATHLIENGADLRAVQELLGHI
                     DISTTQIYTHLAQKKLQKIHAKYHPKG"
     gene            complement(11393..12118)
                     /locus_tag="P5123_05265"
     CDS             complement(11393..12118)
                     /locus_tag="P5123_05265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018305082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HRX15699.1"
                     /translation="MIEIKKLSKVYDVTDEIKVHALKDVDVDIYESEFISIMGPSGSG
                     KSTFMNVLGFLDTQTSGDYILDGFTSQELTDDKKAEIRNLKIGFVFQGFNLLPRTTAL
                     ENVEIPLFYRGGYNMKHAHQKAMEMLDLVGLADRAHHTPAELSGGQQQRVAIARSLVN
                     DPAIILADEPTGNLDTKTTNDILTLFSDLNEKIGITILIVTHEPDVADFTKRKIVFRD
                     GQVTEDVSIKKRSRAGYNREIYV"
     gene            complement(12186..13289)
                     /locus_tag="P5123_05270"
     CDS             complement(12186..13289)
                     /locus_tag="P5123_05270"
                     /inference="COORDINATES: protein motif:HMM:TIGR01730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HRX15700.1"
                     /translation="MSVRFILLNKKVLVLIALVIIAVVVVSTYKSCSEEKGVSYRFDP
                     VELGDVQRTVSVNGSLELFSVDLIRSEVEGQVSKIHKDYNEYVVAGDLLMEIYSESIV
                     ESYEQYVDTYRFARIDLQNSRDLFLTKKKLFEEELISSSEYENSRLSYQKEISSFKRV
                     EKTYLEKKELLDSRMVRAVNAGLIIDCPIKLGDDVATRTLLYRIAPTMEKMILTINID
                     ESDVGIVKKGQKVTFTVSAYPEDIFHGEIDQVTMNPTTIDSIVTYQTTAIVNNKNNSL
                     KPGMTAAATIHVASAKNVLRVPNQAFMVTPQEIETKPGEKVLWKKISSAVDQIPVTPV
                     HIKTGVAGDTYTEIITDKIKKGDEILTGISIER"
     gene            complement(13280..>14002)
                     /locus_tag="P5123_05275"
     CDS             complement(13280..>14002)
                     /locus_tag="P5123_05275"
                     /inference="COORDINATES: protein motif:HMM:NF014385.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TolC family protein"
                     /protein_id="HRX15701.1"
                     /translation="ESARTDLFLAMGLEVDNKTSIEVALLDSVPELIINENQLVRYAQ
                     DHSPSIKMANIATEVARNSYLISRSKRYPSITLFSTLGYENSEFLNSEMGVVERTTDK
                     DNWSPSWTGGVRASMPIYTGGSLPASIDKNESDYKKTQYSKTSTELDVKMSVKKLFSR
                     LVFLKKQIKISKAGVINAQTNLRINERSYESGLVGQDAISDASENLYAAEISLLKNKI
                     DFMISLTELSNIVGIHEELICR"
BASE COUNT         4390 a         2885 c         1981 g         4746 t
ORIGIN      
        1 gtaccaactg accttaatcg gttattgtta ttcaaaaaaa ccttctcttc cgccgaaaga
       61 gtaagttctt tctttattgc catatagtca gatatggaag cttgaacctt tagtattcca
      121 tcgttagaat caacaactcg tcccccaaga gaaataacag catttttgat aacattaata
      181 tctttttcat attcagaatt aatattaaac tcatttgcaa ttgttgttcc cgaaaaaggc
      241 actgaatcat tttctctatt taaggttacc aaagaaacag tcagaacaac agcgaaaact
      301 gcagccacag cccccgaaac aaaaggcttc gatgcgatat attttccaaa tcctgcatat
      361 cgcaaacttg tttgtttaca gcaattaaga gttttagtgg taaaataatc tctatttaaa
      421 atatgaacag cccttaatcc atgcacaaga ctcaaagtct tttctaattt ttgaaattca
      481 gccaactcag atgatgataa cgattttaaa caatcaattg tgttaccgct tgcataacga
      541 tcataaattt cagaaatctt ttcatcaaca catctttttg cgctcattcg actacacctc
      601 tttctattaa acgctgttta agcatcgcac gccctctatg tatccgaact ttaacattcg
      661 aaagcgatat gttcagttct tccgaaatat cttcatagga catctcttct atttctcgaa
      721 gcaaaagaat atttttataa tcatctggta actttgccaa ctcttctctg gctatatcca
      781 ttgcatttga aagttcaaac tgtttctcaa gatcttctct ctcatcctca atgggaagtt
      841 ccccaaaatc ttcatcatcg gatcgaaata atgaaacaat actcatccgt ccgcgttttc
      901 ttttatagtt cttacagtaa ttcgaagtta tactataaag ccatgtagaa aacttggact
      961 ctcccctaaa tttcgctata gaacggaaaa cactgacaaa tatatcctgc gtcgcctcta
     1021 aagcatcatc atagttacca aggtttgaat atgtgtaacg aaaaacccta tcctgatatt
     1081 tttcaattaa aacacccaag agttccgttt caccattctt taccctgtct attatctctt
     1141 cttctgttaa atcagacata tacctttccg gttagttaca cattttaaca aacaattcta
     1201 ataaaacgtc ttttccctac ttttattatg gatgagctag gaagcacaag ctcaaaatcg
     1261 gcgtccaaaa ccgtctctcc atcgatataa acgcctcctc cttgtatcaa tcttctggct
     1321 tcgccatttg attttgccaa tccagcaaga actagcagct taataatcca gattttcccc
     1381 tctttcaata tatcgctttc aattttatac tcttcaatat cgtcaggtac atcttttttt
     1441 acaaaaacag tgttaaattg ctgctcggct tcctccgcta tagcttcatc ataaaattgc
     1501 gcacaaatat ccttggctaa acgaacttta acatctcgcg gatgcactgc tccatcctgc
     1561 attttcattt taatatcatc gatctcttca tatggaacat ctgttaccaa attaaaatag
     1621 gtaaacataa gatcatcaga aattgacatt acttttccga aaatatcagc aggcttttca
     1681 tctataccaa tatagttacc aagagattta ctcattttct gtacaccatc aaggccgaca
     1741 agtagcggca cagtcattat tgactgctgt gagcaaccat actctttttg caaatcgcgc
     1801 ccaactaaca gattaaactt ttgatccgta ccacctaatt cgacatctgc tttaagcata
     1861 acggaatcat acccctgaac caaaggatac ataaactcaa ttatcgaaat tgattttcct
     1921 gttttatacc tttttgaaaa atcatcccgc tcaagcattc tcgcgacatt atagtgcgat
     1981 gttaactcca agacatttgc aaaatccatt tttgaacacc atttagagtt aaattctata
     2041 atggtcttgt caggatcaag tattttgaaa acctgttttt tatatgtttc ggcgttttcc
     2101 aacacctctt ctctgcttaa tttttttcta gtttcagact taccagttgg atcaccaatc
     2161 attccagtaa aatctcctat taaaaagaca acagtatgac ccatttgctg aaaatgcttc
     2221 attttacgta ttagcacagt atgtccaaga tgaatatctg gagctgttgg atcaaaaccg
     2281 gccttgatta taagaggctt ttgtttttct tccgactctt taactttttt atcaaaatca
     2341 tcttcgggta agatctcttc tgttccccgt ttcaacaatg taatatcatt cttgatcatt
     2401 tttttcactt tcctatacat gattgtaaaa ataccagaca acggcacatt atactggcaa
     2461 cctcaaaaaa ttaaattttc aaccaagttg catctgcgag cagtccagca taggaacttg
     2521 cccttgtagg ttgttcttat cagaacaatc ccacgggtaa tacgaaaaat tcctttagaa
     2581 taggcactta aaccatgttg ccctctggaa aatggtaatt tttcgaagtt acctattgtt
     2641 aataaattag gcaggatatc agtcaattgt tttattttcg gtttttttaa aaagaagcac
     2701 cgcggcctgg caggcaattc cctcttcacg accagtaaac cccagttttt ctgttgtagt
     2761 tgccttgatc ccaacgagat cttcactcac ttcaagagct tttccaattt tttttttcat
     2821 caaagccaca tagggaccaa ccttcggctt ttcgcatata attgtggtat caatatttat
     2881 aatctcgaaa ccactttcgt ttaaaatgga tttaactttt tgcaataaaa caatactgct
     2941 gatattttgg tatgttacat cgttatcagg aaagaggcta cctatatccg gtaaagccgc
     3001 tgcaccaaga agagcatcca tcagggcatg aaccaagaca tccgcatcag aatgccctgc
     3061 taacccaagg gggtgcttaa caaccacacc tccaagaaca agatcccggt tttgacaaaa
     3121 ccgatgaaca tcaaaaccag ttccgcatct taaagaccga atcataaatc aaatacatct
     3181 gccagcgagc taagtttcag aacattaagt actttatcgc taacttctgt aatcgtaaca
     3241 gacttatctt ttttcttttg aagctttaaa agactgatca gaacaccaac accagaagaa
     3301 tcgatatagt taacatcacc catatccagt tcaatgtctt tatccgaatc cttacctaat
     3361 aataaaagct gatccttaaa atccttgata gtcatcattt cgatatcacc ggaaacctta
     3421 accaggactg aactctcatt ttctgttatt ttaatgtcca ttaaagaccc cttcatttat
     3481 tattgaggtt atactgatta aataaaccac aagttgaaaa tagttattat tgtgaaaatg
     3541 tcaatttatt ttcgtctcta tcatattcat cctgattatt tgcgaaccca tgaaattact
     3601 taattctaga ctataaacaa tatccgccac acctgcttca aataactctt ttgctttctg
     3661 cgatccattc catataataa tttctaacaa cttgttggta gtgaataatt tggcatgttc
     3721 ctgcttttta ccaaatattt ttaattcttc aaaagccacc cctcttgaaa gaaatactgg
     3781 aggttggttt tcagcaccaa aaggctccaa actgctgatt ttttttataa aagaataatc
     3841 tatattcgaa acgtctatct ctaaatcaat tataatctct ttctttccga agttttcacc
     3901 aaaattaaaa ccttcgataa gagttttcaa ttttaccaaa ttttcattac aaatcgtaaa
     3961 tcctaatgcc tgatcatgtc ctccaaagcg ctcaaaaatg gagctgtgtg gttcaaataa
     4021 ggaataaaaa ccgctgaccg gacttcttcc cgacccctta caataatgat cgtctatttg
     4081 aacaaccact accgctggtt tatcatattc atctaccaat cttgaagcta taagtccgct
     4141 tatcccctct tctattttat caaaccttgt ataaataaca gagcaatcga gaaaatcctc
     4201 atgattttta gatattacac cggcaaccaa gtcttttaca tatttttttc tataatcatt
     4261 caattcactt atagtacata gaatatcaac actgttttgt ggttgtagaa aaaaatcagc
     4321 tgtcaacgcc gttttgccaa atcgaccagg actgtttaac agtggtgcca cctgccaacc
     4381 aattacttta gacgttatac gcgttttgca aaaattttta agctgcacta aagtactgct
     4441 atcgcaatca ttaaactggt caataccttt agctgtaaaa atcctgttct catctctaac
     4501 gggcacaaca tcagcaattg ttccaatagc aatatacggc atgacttcac ggataaaatc
     4561 accaattttt gctgggatca tgaagataga ctcccgatag acatgaacca gggctttttc
     4621 aacctgattt ttttcattct tataaatcag taacaatttt ttaaacaatg ctgaaaacgt
     4681 atttgctcca aaagcctgat tcacaatatt cgtaattgag tgattggtat atctttcaat
     4741 ttctacgatc tctttttcat caattccata atagaatata atgtcttcgt taccagtata
     4801 gcggtgtatc tcctggaaat aaaaaaattc atttttatca tcatcaagag caataccgtc
     4861 cataaaaaaa tgcattgcgt aacaatcatc ctttttaaac aatagagctg ttcttttacc
     4921 atatactggc aaaaatgata gtaaaaaagc ctgacacagt ttaaaagcta ctcctacacc
     4981 tgcaagctcc ttaaacgggt aaccgcaatt atgtagtttg gggttgacta taactgcttc
     5041 aggtaaaaca gtatcaggct catggtgatc acaaacaaga accgaaacat tagacttgcg
     5101 caaaattgaa attggaatga tatctctgat accgcaatca agtgttataa gcagatcgac
     5161 tttttgctca ataaaaattt cggcaacttc atctgttagc ccatattctg aatgctcccg
     5221 atttggataa taacaaaaag tttcgatacc tagcttatca agcagatttt taacaatata
     5281 aagtgaagtc aggccatcaa gatcagaatc agaaaaaata ccaatttttt ttccagaaac
     5341 tgtacttttt ctaaggagat caacagcttg atacatattt aacattaaga atggagaatg
     5401 aagagctgca taggaaggat acatcaaatc tattatacta tttatatcag tataaccgcg
     5461 tcgcataaga atggcaacaa gatcatgatc aagctgactt gtttctgcca agtactttat
     5521 ttgctcggaa tctaccggtt tcaacaacca gttttctttc attgattctc cgttaaaaaa
     5581 tgcgcttttt cattttgatg agattctatc aattcaataa gaatttcatt ttttcttgac
     5641 tcgagaagga ctctcatatc tccaacatag tttatcagtt ttttttctat ttccgtattt
     5701 ttcccagtta tttggcgata aagatactca tcatcaaagc gtaatctggt tttaacagat
     5761 ttaaagtatg aagcaatttg ctcacatctc aacaaggatt tcaacagagc caaccgtttt
     5821 gattttgtgt tttctctgta ctgaaccttt cccactcgac gcccctcgtt gtcttgtata
     5881 attattgcaa ctgaaacagc tccatttgac tctgataccg cacaataaaa tgttaagata
     5941 ttctctaaag gaacgaccga taaatcagcc cccttgaata tatccaactc ttcattatct
     6001 ataacgccta aatctgaatt ttgttcaatt tttttcttga attttacctt ttttctagat
     6061 ttttgaggat ctttttgggt aataattttc cttgcttccc tgataagaga tattacatct
     6121 tcgatcgagc tatcggcaag ttccgcaatt ttttgaactg ttttaccatc ttccaaaaga
     6181 gaaagcactt tatcaatact aagcatcata agatttatcc ttttttaaca ttaaaacaca
     6241 attactatta ttaactagcg actgctatat aaatattttc cacaatttgt acatatacat
     6301 aattcaccgg aattaacgac cgaaagatca tttggaggta cagtgttatt gcagcccata
     6361 caaactccat tttccatctt gacaattgct ttgccatctt tagattcaga aagtttagca
     6421 aaacgagatc gtattttggg ttcaagtaaa agaagaccgg cctcgaactt cttctggttt
     6481 tcttctttaa tggtttgtaa tcggtctatt ctctcattca gttcttttag agtagcatta
     6541 tcactatttg tatttttctc taaactgatc tcttcttctt taatcaattc ctcttcgtct
     6601 gatattttat caaaatttgc aagtaactgc tcttcaataa tatccttttc cgaatttatt
     6661 ttatcaagtt cattagaccc cgccaataac tctttttcgt tttgagcgcc gttaatcttt
     6721 tcttttaccc tacttgcagc agcatcagct tcttccagaa ttaattctaa agatgatgtc
     6781 tcttttttca atttttttaa ttcagataag cgcttatcaa atttttgctt agtataaacc
     6841 tgattttttt gtatttcttc tatgcgtttt ctggctaata taatctcgcc tgtttcacgt
     6901 ataactgcgt cagtatatct ctgcagctct tccatctgcc gtatattttc tttcatcaaa
     6961 attcaccctt ggaaacattt caatggatta ttcgccaacg taaataattt tataccgttt
     7021 gcaacagaag cttttgtcaa gtaatcttgc aaatcgacgg acaaaaaggt catataatgt
     7081 atttcactag aaaaatggct gatgtcaatc aggtttaaac catttacctc ggctaaaaac
     7141 gcgtcatggt actttatatc accgcacata taaagatcga ctttttcttc ggtcatcttt
     7201 tcaacaagag aactaccact gccaggcatt atcgctacag acttgatttt cttgtcaagt
     7261 tctcctgtat aaatcaaata ctcattacca aaagttttct ttacaagtct aagtacatct
     7321 tccatatata attcatcagg aaattctcca tacgcaccat aaccataacc tgtgtttaaa
     7381 tacggatcaa taacacctat tttcaatagg ccaagcatct gtgcgtgttt gttccagtac
     7441 actctatcaa gatttgtatg aatggcatag tgggaaatat tgttttttat taaatagttg
     7501 attatcctgc cagtaaaaga ttcagtcgta acagatttaa tagatttaaa aaacaacgga
     7561 tgatgagtaa taataaggtt acatccaaat ctcaaagcat catctatcac tttttcatga
     7621 atatcaagcg caagaagaac gccggtcaac ttgtcatctg ccctataaac ctgtatgcct
     7681 gaattatcat aagattcctg tatatcttca gggaactttt ttctaatata atcaattaaa
     7741 tccttaacca gcataatatc tgcctcccta acaaactata ttaaagaaaa tctgtactac
     7801 tctatttatt tgattttaaa cttaattgtc aaacaactaa tataacacag atttattagc
     7861 tagtttaata acttataatt ttaccttttc ggaaatatta ttatttgatg aaatataaat
     7921 cacgtattac tcacaagatt ttcttctttg ttacggacat tgtgttctca tataaacaac
     7981 atgcaaagac aatcttccat aacagtatta caataatctg tttcattagc aaattatcaa
     8041 aaatatctaa taatcatgat ttgtagttca gaaattttta cagataagga gagagttaaa
     8101 aacatctata attgaggtca aaccacaagc caataccatt taataaaaaa atactggcgc
     8161 tcagcatatt aacatcgagt ctccatacga aaagaagcgg taattgttgt caaccgcctc
     8221 tttatatgca ttcataattg catcatatcc agcaaaagct gataccaaca ttaatagcgt
     8281 ggatttaggt gtatggaaat ttgttataag tgcatcaatg gatttaattt tatagggtgg
     8341 gtatataaat atatctgtgt ccccactacc aggcacattt acaccattta caaaagtact
     8401 ttcaagaact cttaacgctg ttgttcccac agcaataact cgtctttttt ccagtcgggc
     8461 tttgtttatt tctgttgtta catccttact caaatcataa tattcactgt gcattttgtg
     8521 ctcagatata ttgtctgatc ttactggact gaaagttccc cacgaaacgt aaagagttaa
     8581 atacttgaga attactcctt ttttttcaag tttatccata atttcagaag taaaatgaag
     8641 acctgcggtt ggcgcggcaa cagcacctgt tttgcgagca taaatggttt gataccgttt
     8701 tatatcatct gccgatgcct cacgttgaat atatggaggt agcggaatag aacctatgcg
     8761 catcaacaac tcatctgtga gaggactaga acttttaaca aaaagaaaat cttctttgcg
     8821 ttctactatt tcaagattca aacccggttc agctaatgat tgaatttttt cacctggttt
     8881 aagctttttt gttcgattgc ttatcacata ccattccgat cgatttatca ttcttgccaa
     8941 aacaatctca acctcagcac cgctactcct tttaaaacaa agacgtgcag gaataacctt
     9001 cgcatcatta aatactaaaa catcaccagc attaatgtgt tgataaatat ttctaaaatg
     9061 atcgtgatta attgtttttt cagaacggct aagcaccaat aatcttgaat cttccctatt
     9121 ttctgcaggg ttctgtgcta taaggttttc gggtaaataa aaattataat cttccagttt
     9181 ttgcactatt tcgatcacta ttaaaacttg gtaaattgta cgtctttata aaaaaaactt
     9241 aaaatagtct tataagtctt gccctcttca gccatctctt tagcactgta ttgacacatc
     9301 ccaaccccat gcccccaacc acgaccttca agacgcaatc cgttttttgt acgaaaagca
     9361 ttaaaaaact ggcttttaac tttcccacct ggaatcatca ttctaaaatc attaccagaa
     9421 acattggctc gtccgttttt atgagtaatt attacttcta caactcgtcc ttgatgtttt
     9481 ttaaaagata atcccgtcac ggcacctata ttattgtatc gtgaatcaag aacctttttt
     9541 atttcataaa gtgttaattc tgttttccat ttatagtgcg gtgatgactt tccataagga
     9601 acgaccttat ctgaaagata aggcaaatct tctccatccc aaacatactt gttgtttata
     9661 attctgcctc cacaagtaga atggaaaagc gctagtattg gcttattatt ataagataat
     9721 atttgtcccg cggtctgact taccgccaaa ttgctatttg ttgtttcgcc ttccataccc
     9781 ttataaacct gaaattttgt tgtcgaatca agatcataaa tagaagcgct ttttttattt
     9841 aaaagggtgt gaaaagcata tgttctagca gcaacagctt gggctttaac cgcttcaata
     9901 tgccagttag gcgacatttc agcagataca acacccataa tataatgctc catcgagaca
     9961 acatttatga catatatttt accagtaacg ttatgaagtt caatttttcc tctatacggt
    10021 tttccattaa ctatcagtgg agcatcccac gactcaattt caacaggcga actaagttta
    10081 tctgagaaaa actttatttt ttttgatttt ccatctacaa gaagttttcc cgatttcaaa
    10141 ttttttactt ttattctttt tactgatcca acaaccaccg gcgcatcaga tttatgtagc
    10201 aaaactctta taaatgaaga tttatctgat attacttcac ctcgctgttc taaaaattgc
    10261 tgcgaaggcg tacaggacgt aataaaaata aaaattgtaa tagttataat aaatttgcaa
    10321 ctcatgaata attagccctt tggatgatat tttgcatgaa tcttttgaag tttcttttga
    10381 gcaagatgtg tgtaaatctg agtagttgaa atatctatat ggccaagaag ctcctgtact
    10441 gctcgaagat ccgcaccgtt ttctattaga tgtgttgcaa aagaatgacg aagtgtatgc
    10501 ggtgtaatgt tttttttgat atttgttctc gagacgtagc ctttcaataa tctccataca
    10561 gattttcgat ttaacattga accttttttg cttatgaaga gatactcact ttctctggat
    10621 cccagaatta tttccctaga tctttcaaga taaagtttca tcaaacgaac cgcacgagcg
    10681 cctacaggca caaaacgttc cttattacct tttccagtta ctttaattaa tttatcctca
    10741 aagtctatat caaaaaattt cagctctatg gcttcagaaa ttctcaaccc gcatgaatat
    10801 aataactcaa aaatggtctt gtctcttaac tcgtaacagt cactttctga tatcgagtta
    10861 aacaacgcat caacttcttc aacagaaaga aaatctggaa gtgactttct tatttgaggt
    10921 gtttcaatct ttattgtcgg atttgaaaca tcgagatcag tactagatat aaaattgtaa
    10981 aactgacgta tagcagcaag agatcgggca agtgtccgtg aagagttatt ctttttttgt
    11041 tgctgaaaag aaagaaacct tttaacatct tcactatcag cacttaaaat ggatttattt
    11101 gcactatgaa gaaattcatt aaattttttc aaatcatatg aatatgaata aatactgttt
    11161 tgagacagac ctttttcaac ctttaaaaac cttttaaatt ttaaaatttc cttgttctct
    11221 tccaacgtaa tgctcctggc gataacgtaa ttgactgcag tgactgctga catttatatt
    11281 atcggtcctt taagcaaaaa cgctttatac agtaagacat aattttctta tggaacataa
    11341 aatttttatg tttttggcac aatttatgaa aataatctca aaaagtcata aattatacat
    11401 atatttctcg gttgtaccct gcacgagatc gtttttttat gctaacatct tcggtaacct
    11461 gaccatcccg aaaaacaatt ttcctttttg taaaatcagc aacatccggc tcgtgagtaa
    11521 caatcaatat tgttatacct atcttttcat ttaaatccga aaaaagcgta agaatatcat
    11581 tcgttgtttt ggtatcaagg ttacctgttg gctcatccgc aagaataata gctggatcat
    11641 taacaagaga gcgagcgata gctacacgct gctgctgacc accagagagc tctgcaggtg
    11701 tatgatgtgc tcgatccgct aaaccaacga gatccaacat ttccatcgct ttttgatgtg
    11761 catgtttcat attgtaaccg cccctataaa aaagagggat ctcaacattt tccaaagcgg
    11821 ttgttctcgg aagaagatta aaaccctgaa atacaaaacc tatttttaaa ttcctgattt
    11881 cagctttctt atcatctgta agctcctgag aggtaaagcc atcaagaata taatcacctg
    11941 aagtttgggt atcaagaaag ccaagaacat tcataaaggt agattttccg gatccggaag
    12001 gacccataat tgatataaat tcagactcat aaatatcaac atcaacatct tttaatgcat
    12061 gaactttaat ctcgtcagta acatcataaa ccttcgataa tttcttaatc tcaattatct
    12121 tcttaagttt ttttgtggac tttttacctt ttttaacagt tttcttagat tccttattca
    12181 taaaactatc tctcaatcga tattcctgtc aaaatttcat cacccttttt gattttatca
    12241 gtaattatct cggtataggt atcaccagca actccagtct tgatatgaac aggcgtaacg
    12301 ggtatttggt caacagcaga acttattttt ttccaaagaa ctttttctcc cggttttgtc
    12361 tcaatctcct gaggagtaac cataaaagcc tgattgggaa cccgtaagac attttttgca
    12421 cttgctacat gtattgtagc cgcggcagtc ataccaggct taaggctatt gtttttatta
    12481 ttaacaattg cagttgtctg gtaagtgaca atactatcaa ttgtcgtggg gttcattgta
    12541 acttggtcaa tttctccgtg aaagatatct tcaggataag cactaacggt aaatgtaacc
    12601 ttttgccctt tttttacaat tccgacgtca ctttcatcta tatttatagt taatatcatt
    12661 ttttccattg taggtgctat cctgtataag agtgttcttg tagcaacatc atcccccagc
    12721 tttatgggac aatcaattat taagcctgca ttaactgctc ttaccattct gctatccaac
    12781 agctcttttt tttcaagata tgttttttca acgcgtttaa aactagatat ttctttctga
    12841 taagatagcc ttgaattttc atattctgaa gatgaaatca actcctcttc aaataatttt
    12901 ttttttgtta aaaaaagatc tcttgaattt tgcaaatcta tccttgcgaa acgatatgta
    12961 tcaacatact gctcataaga ttcaactatt gactctgaat aaatttccat aagcagatcg
    13021 cccgcgacaa catactcatt ataatcttta tgtattttag aaacctgccc ttcaacctca
    13081 cttcgtataa gatccacact aaaaagctcc agagaaccat taacacttac agttctttga
    13141 acatcaccca actcgactgg atcaaaccga taactgacac ctttttcttc agaacaagat
    13201 ttatatgtgg atacaacaac aacagctatt atcactaaag caatcaaaac cagtactttt
    13261 ttatttagca aaatgaatct taccgacata tcaactcctc atgaatacca acaatattag
    13321 ataattcagt taacgaaatc ataaaatcaa tcttattttt taaaagagaa atttcagcag
    13381 cataaagatt ttctgaagca tcacttatag catcctgccc aactaaacca ctttcataac
    13441 ttcgttcatt aatccgcaaa tttgtttgtg cattaatgac accagctttt gaaattttaa
    13501 tctgcttttt aagaaaaacg agcctgctaa aaagcttttt aaccgacatt tttacatcaa
    13561 gttctgttga ggttttagaa tactgagttt ttttataatc tgactcattc ttgtcaatag
    13621 acgccggcaa cgatccacct gtgtaaatag gcatactcgc tcgaactccg ccagtccatg
    13681 aaggtgacca attatcttta tcggtagtac gctcaactac tcccatctct gagtttaaaa
    13741 attctgaatt ttcatatcct aatgtactaa aaagtgttat actaggatac cgctttgaac
    13801 gagaaatgag ataactattg cgggcaacct ctgtagctat attagccatt tttattgacg
    13861 gtgaatgatc ttgggcatag cgtaccaatt gattttcatt aataattaat tccggtacag
    13921 aatctaataa ggccacttct atcgaggttt tattgtctac ttcaagcccc atcgctaaaa
    13981 acagatcggt tctggcactt tc
//
LOCUS       DAOTGZ010000054        13733 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_3064
            NODE_5347_length_13733_cov_5.007311, whole genome shotgun sequence.
ACCESSION   DAOTGZ010000054 DAOTGZ010000000
VERSION     DAOTGZ010000054.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868088
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 13733)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 13733)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:22:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,785
            CDSs (total)                      :: 2,751
            Genes (coding)                    :: 2,732
            CDSs (with protein)               :: 2,732
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 12 of 19
            Pseudo Genes (internal stop)      :: 5 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13733
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5347_length_13733_cov_5.007311"
                     /isolate="AMR_MDS_3064"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..360
                     /locus_tag="P5123_05280"
     CDS             <1..360
                     /locus_tag="P5123_05280"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRX15702.1"
                     /translation="DSPSYGDLQITITGIRGNDPVYWGFFNSEPLDSGNVMTKGRSSV
                     ADGKATIYISGLKFGRYAIFGWQDLNEDIRWNSESEGNIIFGKSPKKFGDPLSLEENG
                     FDFDDNNSEYTIEMRYP"
     gene            complement(528..3008)
                     /locus_tag="P5123_05285"
     CDS             complement(528..3008)
                     /locus_tag="P5123_05285"
                     /inference="COORDINATES: protein motif:HMM:NF012772.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HRX15703.1"
                     /translation="MIMFENFSLEIIINLVLPSVSSAISFLLAMMFLYFYRKCGSYYY
                     SSSFVLSLNITLYSICEALIRFNGILFRWYNDAFVFYSIKQVAISLLIFSVSYLFFTL
                     EPKNRYHKGLFMTFMVSGLSMALVSSVLLYFNHPLFLQRPDNIPAALTYANIYRMYEY
                     GILYNIRNIIVMLLTLSSCFIFFIRLIRSGNYYEGGIQFAGVFLLTFFSLSDVLRLYY
                     GYNVCLFPSINFPRTSIGVILFLLLYSFFLVKTFFDKAGLTDQTLMELRESQKNLKLI
                     ARNVNEVVWITDYPPVKTYYINSLFKHIFNIPVKEVYNDCGIWKNAVHFDDRHLFKSS
                     LDFSRITRNSECEYRIYNRAKVAWVRDRIYPVKNRSGEIFRLIRIIEDITEKKQSEEQ
                     LSYLAFHDPLTGLANRKALYETVSDSLLQSARNRYQSMKAFLFIDLDEFKDVNETAGH
                     SVGDKLLCYVSDRLSNTIRETDYIFRIGGDEFVVILNNIDDEYAAASVAEKIIGKLAK
                     PFICDSERLFIGVSIGIAVYPKDGIDIEDLIKNSDIALRDAKKVKNSFSFYNTEMNIR
                     AVNRVLLEKDLRGALDRGEFNLLYQPFINRNGEVCGMEVLLRWKSSHGYVEPDKFIPI
                     IENNGLIVPIGSWVLNQALGFQKSLADMGYKIRIAINISPRQFRDEQFLSIVEKAFEK
                     SGAAKGRVELEITEGLMVEDPEEIATKMKQLSLAGVRFSIDDFGTGYSSLSYIKDFPI
                     SHIKIDRSFVMTIGENESNRKIIKAIIAMAHSLGLEVIGEGIETDEQLAYLAQVECDE
                     IQGYYYSKPLSPESFLEFIKKNPVKSQG"
     gene            complement(3035..3655)
                     /locus_tag="P5123_05290"
     CDS             complement(3035..3655)
                     /locus_tag="P5123_05290"
                     /inference="COORDINATES: protein motif:HMM:NF014724.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcF family protein"
                     /protein_id="HRX15704.1"
                     /translation="MKIVAKLIKRIKQIVVALLLLLFAALIFFTYTVYTVYSYPRDKA
                     VIKTDAVIVLGAAAWGKNPSPVFKERINHAIRLYDAGMCRYIIFTGGKGGEDEPGESV
                     IARRYAIKNGVNEKFILIENKSRTTEENIFYAGKIAEKYGLKTFTLVSDPYHLKRAAV
                     IAEFHGMTVYPSATPTTRFKGSEKKLEFLIRESYYLILYKIGIALM"
     gene            complement(3668..4246)
                     /locus_tag="P5123_05295"
     CDS             complement(3668..4246)
                     /locus_tag="P5123_05295"
                     /inference="COORDINATES: protein motif:HMM:NF015559.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RsmD family RNA methyltransferase"
                     /protein_id="HRX15705.1"
                     /translation="MRVVTGLLKGRNIPFNNSSFDQADVTSGKVKESVFSIIGPVING
                     CSFLDLYGGSGQIGFEAYSRGAKVVINERDKKRYQFIKRCADNFGIAQNIKILNFADE
                     VCIRYLFKREYSFDVVYLDPPYVKKRGKVSLYGQTLSSLERRGILNKGCQILIQYHSK
                     NELVSIPEMFVTESVHVYGATSLALYIYSPGL"
     gene            complement(4247..4441)
                     /locus_tag="P5123_05300"
     CDS             complement(4247..4441)
                     /locus_tag="P5123_05300"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="disulfide oxidoreductase"
                     /protein_id="HRX15706.1"
                     /translation="MKIELDTTVEEALKMPGSAGKVFKKYDLYCAGCHGSSQDTISRV
                     IINNGLDPEKFLNDLNSSIE"
     gene            4671..4859
                     /gene="rpmB"
                     /locus_tag="P5123_05305"
     CDS             4671..4859
                     /gene="rpmB"
                     /locus_tag="P5123_05305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008981056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L28"
                     /protein_id="HRX15707.1"
                     /translation="MAKCEICGKSVQFGCNVSHSHKRTNKMWKPNIKKVRVLQAGSKV
                     RKYVCTQCIRSGKVTKAV"
     gene            5071..5409
                     /locus_tag="P5123_05310"
     CDS             5071..5409
                     /locus_tag="P5123_05310"
                     /inference="COORDINATES: protein motif:HMM:TIGR00377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043856 - anti-sigma factor antagonist
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="STAS domain-containing protein"
                     /protein_id="HRX15708.1"
                     /translation="MAALTYSREKRGKSVVVKIGGSLDAKTAPTLDKKLSDEVDQVPE
                     KLVISMEKLEYIASAGMGTLINYNEIMSKKGKKLILCDLPDKITKIIKMLGFINFFSI
                     CKTLDDAENG"
     gene            5402..5860
                     /locus_tag="P5123_05315"
     CDS             5402..5860
                     /locus_tag="P5123_05315"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: protein motif:HMM:NF024970.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HRX15709.1"
                     /translation="MGNNLFLQKKIKRNLDELDSFRESFLCYLTENGIEEETASHFEL
                     CAYEVIANIIEHDSAIPGFCDDIQVIFSAEKNYGQVILNYSGPEFDLTKRPLPDISTH
                     FKEGLKRGLGIYIIHKLMDEIWYTHNEGENSLTMKKSIPDNDRIKIEQIL"
     gene            complement(5857..6765)
                     /locus_tag="P5123_05320"
     CDS             complement(5857..6765)
                     /locus_tag="P5123_05320"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRX15710.1"
                     /translation="MEQIEKIKLPLIKDYIKTIKSLAGQGRNYYHKFLVIPLSSWSSH
                     PVDYELSDLQKEIEEGRSIPDIEKIAVSYKRALIRSVAKDQFDLTLPGKLSISYLLDI
                     QNSIDLANRMESLQFVNLLRKPFTDYSRIEYNSMNTRFRQELDSIQDNGELIESISKL
                     KSDEEEKLKFELDRSEKMVCAIKSRLNQLDASDIRKHIIAYLISTASPSMPRLHKDID
                     PVIDKIGRLHRGYKKEIYDSVAVIIYRELMAAVKKSDLRKAILLISKFTVLFRGNPGT
                     PHFEEVDAFEKIFFSIIEKKNLWDLI"
     gene            6938..7237
                     /locus_tag="P5123_05325"
     CDS             6938..7237
                     /locus_tag="P5123_05325"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRX15711.1"
                     /translation="MKFNIKAFTISSVIVCTLPAFILFTWCAINNFAIEAVVLFETIH
                     PSGAFSLTNTISSSFTAKIPGIIFNTLYVAVDTFIFTFLFALLYNLLSGNNKKAR"
     gene            7241..9370
                     /locus_tag="P5123_05330"
     CDS             7241..9370
                     /locus_tag="P5123_05330"
                     /inference="COORDINATES: protein motif:HMM:NF018884.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016791 - phosphatase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SpoIIE family protein phosphatase"
                     /protein_id="HRX15712.1"
                     /translation="MRRKLKNVRPIRIGLRLQISLIMIFSIALISFILSVAIFKKSEQ
                     TVIKEITHFSGAILKGIKRETKLYLYYDREVRNFKKSGESRPYLNDCKTNRDEAIQKV
                     MQFFPDIISRDTMLDVAFLINIIETDNTSESKRNRFLYFDRNHARVDDAKNSYDEELI
                     QLLDFFLETIETGVNFKQITTQNKKIDQDDSVVVGLPVFSGDFTEELYNKYKDFYKSL
                     TRSLPANRETSSLQEQHSYFEQEFIKRLVYNYTDFDYKINAANDKDYNRLFVYFSAVR
                     YTGRLTNNQREIAQKEFTEQLKSLKSDLSFQNYSETCDQFRKKYNLRLKPNIHNWTGL
                     YRFLERNSISVSSPVPIQELALQAYKSDLNGIIGIRLLSKEFFVNLNRNRNEVTNLAL
                     AIFLRFIIIALLFPGFIIRKINKLADGAYEIGRGNFDYKFKMRGSDEVGRLSDIFNIM
                     GKNLKKAREEMIEKNRMSTELKTAHEIQSVLLPSVLPDINKLQFSSYYASQSEAGGDY
                     YDVIPLAPEKLALVIADVSGHGVGSGLVMTITRTLIHSFSSETVKPSTLMEKLNEHLY
                     LNTASNFFVSMFYAVYDTKSHSLNFSSAGHNPALIVRKNSVKELPAGGVALGAVGRES
                     FERFSSGKNTILEKNDLLVLYTDGIVEAMNNNNEEYGEERFYNVLKKNHDKDAETVKD
                     AVIKDLKSFTSGHEQSDDITMLLMRIT"
     gene            9367..10464
                     /locus_tag="P5123_05335"
     CDS             9367..10464
                     /locus_tag="P5123_05335"
                     /inference="COORDINATES: protein motif:HMM:NF013618.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglycosylase SLT domain-containing protein"
                     /protein_id="HRX15713.1"
                     /translation="MKKIILFLTILCFSQAKADIVLSSLIKTQSEKLEGNSADIPVSE
                     GKDILTAIEDLSALNIKAVRNQVYHYATSGKAFYLSAVKNAEIYRPVIEDIVNGYPEI
                     PIVISDLPILESSYNPYAVSHAGAAGLWQFMPKTATIFNLSINRWEDGRRCLINSTHA
                     AMQHLYHLYKHHKSWELALAAYNCGSTRINQAVANHPQMTYWEIVETGALPTETSLYV
                     QRFAALSILYRHNSFFGLPSTPEQNVELFGIPLKKPVSIPNVSEKTGIPESTIYLFNP
                     QLKKSITPLVGEGYVLLIPENYYESFVNNEDKIYTLPYSRIKTYTVVKGDHLSRIAKK
                     HNTSIDQLIHINKLKKPYIIRPGNQLLIPLH"
     gene            10657..11247
                     /locus_tag="P5123_05340"
     CDS             10657..11247
                     /locus_tag="P5123_05340"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRX15714.1"
                     /translation="MWYYYKYFIIAYIILGSTAIAIVYQFIERVIPDLRVGTYQVNLT
                     IQERSFLMDYFTKAAIDVNNDGIVQVGLTPMSDYMQLRISIANQANSMLILKQDVFAE
                     HAPLGAFASLDHIINKDHSSIISNPEVIATNRKTGEKHVYGFPLSGNKIFRNMDLPES
                     NLYIVMLRRDIKIAIENQSFFDNAEKVIKMIMDDSI"
     gene            11444..12784
                     /locus_tag="P5123_05345"
     CDS             11444..12784
                     /locus_tag="P5123_05345"
                     /inference="COORDINATES: protein motif:HMM:NF014207.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook-length control protein FliK"
                     /protein_id="HRX15715.1"
                     /translation="MENSTISNYKAQILVPDFINNVNKHKSSNNQEFNDFDFKNMLKQ
                     AVEQNDPYKELHRNNAPVEQQLQNDYTVQKPEQNYDSRSEKTEDIKADDKISYTKNTE
                     KSEELDSEQNSSIDKSAKTNESEKNEINDEQISELKKELHSLLQTLSKGVSPANNKDT
                     VAKIDKLSEKIENLLDMDHSKGSKKLINLKHQLSNLHKNLELLKKEVLNPDSKTPLLA
                     DLIKKTGLVLEQLTAKDRIALSRANHNDNSSANKINLETAVNSTGEKEIKQSNSLKND
                     DNSFSFTKDSSQSKNDLSSIKADRQPMKSDGLFKDQINDLINKARVTVTDKSNGTINL
                     RMYPERLGNVTVNLGLENGILNGKFLVDSKEAKDMLLSQFDSLKERLLEEGINLGSFS
                     VDVRNGESSYNNYKESKELYEALTAPVVHSAAEEYSQISSQSGQYYDGSINLVM"
     gene            12792..13484
                     /locus_tag="P5123_05350"
     CDS             12792..13484
                     /locus_tag="P5123_05350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004430853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook capping FlgD N-terminal
                     domain-containing protein"
                     /protein_id="HRX15716.1"
                     /translation="MPTDNVQFNNKLSPQELAKARNQAEFINKKHQADGKSYGEGLGK
                     DSFLQLLVTELTHQDPTNPMQDRDFIAQMAQFSSLEQMTNVNTQISELNSRGKLNEAY
                     SLIGKRVQALDAQRGIPVDGTVSHVVRRQGEIRVVVDKYECRVDDVHAVFPSPQEIQK
                     SSQTLKTDTDNQPVKNAPVIDSNVNTQKSTPEMDFTTTEKGVRNSFNEFYNYYDNNIN
                     GYSKAAKSYSAY"
     gene            13507..>13733
                     /locus_tag="P5123_05355"
     CDS             13507..>13733
                     /locus_tag="P5123_05355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002660579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body protein"
                     /protein_id="HRX15717.1"
                     /translation="MMRSLFSGVSGLKNHQTRMDVIGNNISNVNTYGFKTSRVSFQDM
                     LSQTISGAARPEEERGGVNPKQVGLGMTVAS"
BASE COUNT         4645 a         2778 c         2324 g         3986 t
ORIGIN      
        1 gattcaccca gttacggtga tcttcaaatt actataacag gaatacgcgg aaacgatcct
       61 gtttactggg gattttttaa cagcgaaccg ctagactcag gaaacgtaat gactaaggga
      121 cggagtagtg ttgcagatgg aaaagcaaca atatacataa gcggattaaa attcggcagg
      181 tacgccatat tcggatggca agatttaaac gaggatatta gatggaattc tgagtcggaa
      241 ggtaatatta tctttggaaa atcaccaaaa aaatttggtg atccactttc acttgaagaa
      301 aatggattcg attttgatga caataacagc gaatatacaa tagagatgag atatccatga
      361 tatttatctc ttcaattaac aagaaagcga tgatatagtt catcgctttc ttgttataca
      421 aattctggag gacaacagtt actaaaactt aatattttta gaatcctgtt tataataaat
      481 ttttaaaata ctccgtttaa aaattcaatc aataaaatca aactaagtta gccctgactc
      541 tttactggat ttttctttat aaactctaga aaactctctg gagatagagg tttgctgtaa
      601 taatagccct gtatttcgtc acactctacc tgagcaaggt atgccaattg ctcgtcggtt
      661 tcgatacctt cgccaatcac ctcaagaccc aaagaatgcg ccatggctat tattgccttt
      721 attatcttac ggttgctctc gttttcacca atagtcatta caaacgagcg gtcaattttg
      781 atatgcgaaa tagggaaatc ctttatgtaa cttagagaag agtaaccagt gccaaaatcg
      841 tcaatggaaa accttacacc cgctagactc aactgcttca tctttgtggc tatctcttcg
      901 ggatcttcga ccattaaacc ctctgttatc tctaactcca ctctcccctt tgcagcacca
      961 cttttttcaa aagctttctc gacaattgac aaaaactgct catctctaaa ttgtcgcgga
     1021 gaaatattta tggcaatacg tattttgtaa cccatatccg ctaatgattt ttgaaaacct
     1081 agggcctgat ttaacaccca tgacccaatt ggaacgataa gaccattgtt ttcaattatc
     1141 ggaataaatt tatcaggttc tacataacca tgagaacttt tccagcgcaa aagaacttcc
     1201 atgccacaaa cttcaccatt acgattaata aacggttgat acaaaagatt aaattcacct
     1261 ctgtctagtg cacctcttag atccttttcc aaaagtaccc tattcacagc tctgatattc
     1321 atctcggtat tataaaaaga gaagctgttc ttaacctttt ttgcgtccct aagagcaata
     1381 tcactattct tgatcagatc ttctatatca ataccatctt tcggataaac tgcgattcct
     1441 atgctcactc caatgaaaag gcgctcgcta tcacatataa agggctttgc aagttttcca
     1501 ataatctttt cagcaaccga tgccgcagca tactcatcat caatattatt cagaattaca
     1561 acaaactcat cgccaccaat acggaaaata taatcagttt cacgaatagt gtttgaaagg
     1621 cggtcgctaa cataacagag caatttatca ccaacgctgt ggcctgctgt ctcgttaaca
     1681 tctttgaatt catcaagatc aataaacaga aacgctttca ttgactgata acggttacgc
     1741 gccgattgaa gaagcgaatc agataccgtt tcataaagag ctttacggtt ggctaatcct
     1801 gttaacggat cgtgaaaagc aaggtatgat agctgctcct ctgactgttt cttttctgta
     1861 atatcttcta ttatacgtat aagtctgaaa atttcaccgg agcgattttt aaccggatag
     1921 atacggtcac gcacccaggc gacctttgcc ctgttataaa ttcggtattc acactcgcta
     1981 ttacgtgtta ttcggctaaa atccaaagaa ctcttaaaca gatgacgatc atcaaaatga
     2041 acagcattct tccatattcc gcaatcatta tagacctctt taaccggaat attgaaaata
     2101 tgtttaaaca atgaatttat atagtaggtc ttaactggcg ggtaatccgt aatccacacc
     2161 acctcattca catttctcgc gataagctta agattcttct gactctcccg taactccatc
     2221 agtgtttgat ctgttaaacc tgccttatca aaaaatgtct taacaagaaa aaaactgtac
     2281 agtaaaagga aaagaataac accaattgat gtgcgtggaa aattaatact gggaaatagg
     2341 catacattgt atccataata taaccgcaaa acatcactta aagaaaaaaa agtcagcaga
     2401 aaaacaccag caaactgaat gcctccctca tagtagtttc cagatcttat cagtcttata
     2461 aagaatataa aacatgagct taaggttaac agcattacaa taatgtttct aatattatac
     2521 aaaataccgt attcgtacat gcggtaaata tttgcgtagg tcaatgcagc aggaatatta
     2581 tctggtcgtt gtaaaaaaag tgggtgatta aaatatagca acacagagct aaccagcgcc
     2641 atagacaaac cactgaccat aaatgtcata aacaaccctt tatgatatct atttttaggt
     2701 tccaaagtaa aaaacagata tgacacagaa aatatcaaca acgatatagc cacctgcttt
     2761 atcgaataga acacaaatgc gtcgttgtac catctgaaga gaattccatt aaaacgtatt
     2821 aacgcctcgc aaatactgta tagagtaata tttagcgaaa ggacaaacga tgaactgtaa
     2881 tagtaagagc cacacttgcg gtaaaaatag aggaacatca tggcaagcaa aaaagatata
     2941 gccgatgaca ccgacggtaa taccagatta attattattt ccaaactgaa gttttcaaac
     3001 ataatcattt atcatcgcat tatatgcaat taattcacat taatgcgata cctattttat
     3061 atagtatcag ataatagctc tctctgatta gaaattcaag ctttttctcg cttcccttaa
     3121 aacgggttgt cggtgtagcc gatggataaa cagtcatacc gtgaaattcg gcgataacgg
     3181 ctgccctttt caaatggtag ggatcagaaa ccagcgtaaa ggtttttaaa ccgtacttct
     3241 cggcaatttt tcccgcataa aaaatattct cttcagtagt tctagattta ttttcaataa
     3301 gtataaactt ctcatttact ccatttttta tcgcataacg ccttgcaata acagattctc
     3361 cgggctcatc ttcaccgcct ttgccaccag tgaaaataat gtatcgacac atacccgcgt
     3421 catacagacg tattgcatga ttaattctct ccttgaatac cggcgaggga ttttttcccc
     3481 acgctgccgc ccccaatact atgacagcat ctgtctttat tacagccttg tcgcgaggat
     3541 atgaatagac agtatatacg gtatacgtaa aaaaaatcag agctgcaaaa agtaacaaca
     3601 acaaggcaac cacgatctgt ttaattcttt ttatcaattt tgcaactatt ttcatataac
     3661 ttttgcctca caaaccgggc gaataaatat aaagagccaa agaagtcgct ccatatacgt
     3721 gaaccgattc tgtaacaaac atttcaggaa tagaaaccag ctcgtttttt gaatggtatt
     3781 gtataagtat ttggcaaccc ttattcagaa taccacgccg ttcaagcgaa gagagcgttt
     3841 gcccgtataa actgaccttt ccacgttttt tgacatacgg cggatcaagg tacaccacat
     3901 cgaaactata ttcccttttg aaaagataac ggatacagac ttcatcagca aaatttaaaa
     3961 tctttatgtt ttgggcaatc ccaaaattat ccgcacatct ctttataaac tgataacgct
     4021 ttttatctcg ctcgtttata acaactttag cccctctact gtaggcctca aatccgattt
     4081 gtcctgaccc tccataaaga tcaagaaagg aacatccatt gataaccgga cctataatcg
     4141 aaaaaacgga ctcctttact ttaccggatg ttacgtcggc ctgatcaaac gagctgttgt
     4201 taaaaggaat attgcgtcct ttgagtaaac cagtaactac cctcatctat tctatggaac
     4261 tattcaggtc gtttaaaaat ttttccggat caagtccatt attgataata actctagaaa
     4321 tagtatcttg cgacgaacca tgacagccag cacaatacaa atcatatttt ttgaagacct
     4381 taccagctga accaggcatt ttcagagcct cctcaactgt tgtatcaagt tctattttca
     4441 tctcgattat cctaattatg gcctgagtat atggcacttt ttcccaataa tcagatggtg
     4501 tttaacctgt gtcaattgtt attcataact aaaaccaaaa ccgaaagatc ttttgaacac
     4561 atatcacgac aacaattcag ttctttttat tatctatcaa aattattttc ttgcaaaaat
     4621 tcaacgatat agcaaacatg actgcaaata atcatcaaat ggggaatcca atggcaaaat
     4681 gtgaaatctg tgggaaatca gtgcagtttg gatgcaatgt cagccactca cataaaagaa
     4741 ctaacaaaat gtggaagccg aatataaaaa aagtacgcgt ccttcaggct ggaagcaaag
     4801 tgcgcaaata tgtttgtact caatgcatac gttcgggaaa agtgacaaaa gctgtataag
     4861 ctctcaaaac aattcatcta ttaatgtact tatgacagcc ggggatattc ccggctgtcg
     4921 tgttttatcc ttatccacat tattaacggc atcaacgatt taaccttgac atactcgcta
     4981 tatcttgcac taattacaga aagcttcgat aattataaaa ctctaaacag caaattctcg
     5041 attcgtttaa ggtttttttc aggaggtttc atggctgcat taacttattc tcgagaaaaa
     5101 cgcggaaagt cagtagttgt caaaatcggc ggatcacttg acgcaaaaac cgcgccaaca
     5161 ctggataaaa agctttcaga tgaagttgat caggttccgg aaaaacttgt tatttccatg
     5221 gaaaaactag aatatattgc cagtgccggt atggggacat taatcaatta taatgaaata
     5281 atgtcaaaaa aaggtaaaaa actgatttta tgtgatcttc ctgacaaaat tacaaaaatc
     5341 ataaagatgc ttggttttat caactttttt tcaatatgca aaactctgga tgacgcagaa
     5401 aatgggtaac aatctttttt tgcaaaaaaa gattaaaaga aaccttgatg aactggatag
     5461 tttcagagaa tcatttcttt gctatctcac agaaaatgga attgaggaag agactgcttc
     5521 acattttgaa ctttgtgcct acgaagtaat tgccaatata atagagcacg actctgcaat
     5581 tccgggattt tgtgatgata ttcaggttat cttttcagca gaaaaaaatt acggtcaagt
     5641 cattctaaac tatagcggtc ccgaatttga tctcaccaaa agaccccttc cagatatctc
     5701 tacccatttc aaagaaggcc tcaagcgcgg tcttggcatc tatataattc ataaactgat
     5761 ggatgaaatt tggtacactc ataatgaagg cgaaaacagt ctgaccatga aaaaaagcat
     5821 tcccgataat gatcgaataa aaatagaaca aatcctttaa attagatccc acagattttt
     5881 cttttctatg attgaaaaaa atatcttctc gaaagcatcg acctcttcaa agtgtggagt
     5941 ccccgggttg cccctgaaaa gcaccgtaaa cttcgaaatc aggagtatag cttttcgcaa
     6001 atcagatttt tttactgcgg ccataagttc tctatatatt ataacagcaa cagaatcgta
     6061 aatttctttt ttgtaacctc tatgcaatct acctatcttg tcgattactg gatcaatatc
     6121 cttgtgtaac cgaggcatgg aaggtgaagc agtactgatc agataagcaa ttatatgctt
     6181 cctaatatct gatgcgtcaa gctgattcaa acgagatttt attgcacaca ccattttttc
     6241 agatcgatct aactcaaact taagcttctc ctcttcatca gacttcaact ttgatatact
     6301 ctcgataagc tcaccattat cttgtattga gtctaactcc tgccgaaaac gggtattcat
     6361 agaattatac tctatacgag aatagtctgt aaacggtttg cgtagcaaat ttacaaattg
     6421 gagggattcc attctattcg ccaaatcgat gctgttttga atatcaagaa gataacttat
     6481 ggataatttc ccgggcagag tcagatcaaa ctgatcctta gctaccgatc taataagggc
     6541 acgtttatat gaaactgcaa tcttttcgat atccgggatg gaacgaccct cttcgatttc
     6601 tttttgaaga tctgataatt cataatcgac aggatgacta ctccatgagg agagcggtat
     6661 gaccagaaat ttatgatagt agtttcttcc ctgaccagcc aacgacttaa tggtctttat
     6721 gtagtctttt ataagaggga gcttaatctt ttcgatctgt tccaaaacta ccccgctgat
     6781 aacagtgtac gttgcaattg tacatattaa catcccaggc agcaaagatg tcaatgacaa
     6841 ttttcattga cgtataacaa agaacttact ttaatgggaa agtctcaatt tcacatattt
     6901 gactgaaaca cataatttga cccgggagag tatttccatg aagttcaata tcaaagcgtt
     6961 tactatttca tctgttattg tctgtacctt acccgcgttt attctgttta cctggtgtgc
     7021 cataaataat ttcgcaatcg aagcagttgt tctttttgaa acaattcatc cgtcaggcgc
     7081 tttttctctt acaaatacaa tatcgtcatc atttacagca aaaattcccg gtattatatt
     7141 caataccctt tatgttgcag ttgatacctt catctttact tttctcttcg ctcttttata
     7201 taacttgcta tcgggcaata acaagaaggc tcgttagtat atgagacgaa agctaaaaaa
     7261 cgtaagaccc atacgcattg gtctgcgcct tcaaatatcc cttataatga ttttcagcat
     7321 agcgttgatc agtttcatac tctcagttgc catattcaaa aaaagtgaac aaaccgttat
     7381 taaagagatc acacattttt cgggtgcaat tcttaaggga atcaagcgtg agaccaaact
     7441 ttacctctat tatgacaggg aagtcagaaa tttcaaaaaa agtggtgaat cccgccccta
     7501 tctgaatgat tgcaaaacaa atagagacga agcgatacaa aaagttatgc aattttttcc
     7561 tgatataata tctcgggaca cgatgcttga tgtggcattc ctaatcaaca ttatcgagac
     7621 agataacacc tccgaatcaa aaaggaaccg ttttctgtat tttgaccgaa atcatgcccg
     7681 agtcgatgat gcaaaaaaca gttacgatga agagcttatt cagttgttag acttcttcct
     7741 cgaaacaata gaaaccggtg tcaactttaa acagataacg acccagaaca aaaaaattga
     7801 ccaggacgat tcggttgttg ttggattacc tgttttttcc ggtgatttta ccgaagagtt
     7861 atacaataaa tacaaagact tttacaaaag ccttacgcgg tctcttccgg caaatcgaga
     7921 gaccagttca cttcaagaac aacacagcta ttttgaacag gaattcatca aaagactggt
     7981 ttataactat accgattttg attataaaat caatgccgct aatgataaag attacaacag
     8041 attgtttgta tacttctcag cagtccgtta tacggggagg cttacaaaca atcaacgaga
     8101 aattgctcaa aaagagttca cagagcagct aaaatctcta aaatccgact tgtcatttca
     8161 aaattattct gaaacgtgcg atcaatttcg aaagaaatat aatcttcgct tgaagcccaa
     8221 tattcacaat tggaccgggc tttatcgttt tcttgaacga aactctattt ccgttagttc
     8281 gccagttcca atacaagaac ttgcgcttca agcatacaag agcgacctca atggcataat
     8341 aggcatacgc cttctaagca aagaattctt tgtgaatttg aacagaaaca gaaacgaagt
     8401 aaccaatctt gcattagcca tttttttacg ttttataata attgcactgc ttttccccgg
     8461 ttttattatc agaaaaatta ataaactagc tgatggtgcc tacgaaatag ggcgcggcaa
     8521 ttttgactat aaatttaaaa tgcgtggaag cgatgaagtt ggacgtcttt cggacatctt
     8581 taatataatg ggcaaaaacc tgaaaaaagc tcgtgaagag atgatcgaaa aaaaccgcat
     8641 gtctaccgaa ctaaagactg ctcacgagat acaaagcgtt ttactaccct ccgtactgcc
     8701 tgacataaat aaactgcaat tttcttcata ttacgcatca caatcggaag caggcggtga
     8761 ttattatgac gtgatacctt tagcacccga aaagcttgcc ttggttattg cagatgtatc
     8821 aggccacggt gtcggttcgg gtcttgttat gaccattacc agaaccctta tacacagttt
     8881 ttcttcagag acggtcaaac cttctactct tatggaaaaa cttaacgaac atctttattt
     8941 aaacactgcc tcgaattttt tcgtctcgat gttttacgcc gtgtacgaca caaaatcaca
     9001 ctctttaaat ttttccagtg ctggtcacaa ccctgcgctt atcgtcagga aaaacagcgt
     9061 taaggagctt ccagcaggcg gtgttgcact tggagctgtt ggtcgtgagt cttttgaaag
     9121 attttcttcc ggcaagaata cgatccttga aaaaaatgat cttcttgttt tgtataccga
     9181 tggaatcgtc gaagcgatga acaacaataa cgaagagtat ggagaagagc gattttataa
     9241 cgtactaaaa aagaatcatg acaaagacgc cgaaacagta aaagatgcag taataaaaga
     9301 tctcaaatct ttcacatccg gtcacgagca gagtgatgat attacaatgc ttttaatgag
     9361 aatcacatga aaaaaataat tttatttttg actatactat gcttttccca ggcaaaagca
     9421 gatattgttc tatcaagttt aatcaaaaca caatctgaaa agctggaagg taactcagcc
     9481 gacatacctg tgtctgaagg caaggatatt cttacagcta ttgaagactt atccgctctg
     9541 aatattaaag ctgttcgcaa tcaagtttat cactacgcca caagtggcaa agcgttttat
     9601 ctttctgctg taaaaaatgc cgaaatttac aggcctgtaa tcgaagatat cgtaaacggt
     9661 tatccggaaa taccaattgt tatttctgat cttcccattt tagaaagtag ctacaatccc
     9721 tacgctgtat ctcacgctgg agcagccgga ctttggcaat tcatgcccaa aactgccact
     9781 atttttaatc tttcaataaa cagatgggaa gatgggcgaa gatgcctaat aaattcaaca
     9841 catgcagcga tgcaacatct gtatcatcta tataaacatc ataaatcctg ggaacttgct
     9901 ctcgctgcct ataactgcgg atcaacaaga ataaatcagg ctgttgccaa tcatccccag
     9961 atgacatact gggaaatagt tgaaacggga gcattaccaa cagaaaccag cctttatgta
    10021 caaagattcg cggccctatc aattctttac agacataaca gtttcttcgg actaccttct
    10081 acccccgaac agaacgttga actttttgga attccattaa aaaaacctgt ttcaatacca
    10141 aatgtctctg aaaaaacagg catacctgaa tctacaatct atctttttaa tcctcaatta
    10201 aagaagtcca taacccctct tgttggggag ggttatgttt tactgattcc cgaaaattat
    10261 tatgaaagtt tcgttaataa cgaagataaa atatacactc ttccctattc gagaataaaa
    10321 acttatactg tagtaaaagg cgaccacctg tcacgtatcg caaaaaaaca caacacatcc
    10381 attgatcagt taatacatat taataaacta aaaaaaccct atattattcg tcctggcaac
    10441 caacttctta ttccactcca ctaagatata tccaaataaa gaatatcttg acagatatcg
    10501 gcacaaaaag tgatatccaa tggtataaca cgaacgaaga acttatttaa atatgttcgt
    10561 tattttttac gccggtacat tttatagctt ttataaatat tcaagacata cggagcgcaa
    10621 atcatgctta agcggtttat aaagtggtgt gacaatatgt ggtattacta caaatacttc
    10681 ataatagctt acataatttt aggctctacc gcgatagcta tagtttacca gtttatcgaa
    10741 agggttatac ccgatctaag ggttggcact tatcaagtaa atcttactat ccaagaacgc
    10801 agttttctaa tggattattt taccaaagct gcaattgacg tcaataatga cggtatcgta
    10861 caggtgggtc ttactcccat gtcagactac atgcaacttc gaatttcaat tgcaaatcag
    10921 gctaactcca tgcttatatt aaaacaggat gtttttgcag aacatgcccc cctcggtgct
    10981 tttgcgtccc ttgaccatat tattaataaa gatcactctt ccataatatc caatcccgaa
    11041 gttattgcaa caaatagaaa aactggtgaa aagcatgttt acggctttcc gttaagtggc
    11101 aacaaaattt tcagaaacat ggatcttccc gaaagcaatc tgtatatcgt aatgcttagg
    11161 cgagacatta aaatagcgat tgagaatcaa agctttttcg ataacgccga aaaagtgatt
    11221 aaaatgataa tggatgactc tatttagctc aaataataca cagaattttc ttctctttca
    11281 gtaatctaat accgtccata ttttatctat tttcacgtaa attatccttt agaaaaattt
    11341 tttttcttac gatacctcag gtaagaggtc ttccgattaa cctcttattt gactaaaccg
    11401 ccttctgttt tttacagaaa atagcggtta caatgaggtc tcgatggaaa acagcactat
    11461 ttctaattac aaggcacaaa tactagtacc tgattttata aacaatgtta ataaacataa
    11521 aagcagtaat aatcaagaat tcaatgattt tgactttaaa aacatgctta aacaagcagt
    11581 cgaacagaat gacccatata aagaacttca ccggaataac gcacctgttg aacaacaact
    11641 ccaaaacgat tacacggttc aaaaacccga acagaattac gattccagaa gtgaaaaaac
    11701 agaagatata aaagctgatg ataagatttc ttataccaaa aataccgaaa aatcagaaga
    11761 attagactct gaacaaaatt cctctatcga taaatcagca aaaaccaatg aatctgaaaa
    11821 aaatgaaatt aacgatgagc aaataagtga attaaaaaaa gagctacact cccttttaca
    11881 gacactgtca aaaggggtat ctcccgctaa caacaaagat acggtagcca aaatagacaa
    11941 gttgtcagaa aaaattgaga acctgcttga tatggatcat agtaaaggca gcaagaaatt
    12001 aattaacctt aaacatcagc tttccaacct gcacaagaac ttggagctgc taaaaaaaga
    12061 agtactgaat cccgattcca aaacaccgtt attagccgat cttatcaaga aaactggtct
    12121 tgtacttgaa caactgactg caaaagatcg tatcgctctt tctcgggcaa accataacga
    12181 taacagtagc gcaaacaaga ttaatcttga aacggctgtc aattccacag gagaaaagga
    12241 gatcaagcaa agcaactcat taaaaaacga tgacaactct ttctctttta caaaagatag
    12301 ttctcaatcc aaaaatgact tatcatccat aaaagccgat agacaaccca tgaaatctga
    12361 cggattattt aaggatcaga taaatgatct tataaataag gcaagagtaa cggttactga
    12421 caaaagcaac ggcacaataa accttagaat gtatccagaa cgtcttggaa atgtaactgt
    12481 taaccttggt ttggaaaacg gtattcttaa cggtaaattt ctggtcgatt caaaagaagc
    12541 caaagatatg cttttatcgc aatttgattc acttaaagaa cgccttcttg aagagggtat
    12601 aaaccttgga agtttttccg ttgatgtaag aaacggcgaa tcatcataca acaactacaa
    12661 agagagcaaa gaactttatg aagcattaac tgcacccgtt gttcatagcg cagctgaaga
    12721 gtactcacag atatcatcgc aatctggaca atattatgac ggctcaatca atcttgtaat
    12781 gtgaggtaat catgccaact gataatgtac agtttaataa caagctttca ccacaagagc
    12841 ttgcaaaagc cagaaaccag gcggagttta tcaataaaaa acatcaggcc gacggtaaaa
    12901 gttacggtga gggtcttgga aaagattctt ttcttcaact tcttgttacc gagctcactc
    12961 atcaggatcc gacaaatcct atgcaagaca gggatttcat tgctcagatg gcacagtttt
    13021 cttctttgga acagatgaca aatgtaaata ctcagataag tgaactgaat tctcgtggca
    13081 aactaaacga ggcgtattct cttattggta aaagagtaca ggcacttgat gcacagagag
    13141 gaattcctgt agacggtact gtatcccatg ttgttcggcg tcagggcgaa ataagggttg
    13201 ttgtagataa gtatgagtgc agagtcgatg atgtacatgc ggttttccca tcgccacaag
    13261 aaatacaaaa aagcagtcag acattaaaaa ccgatacgga caatcagcca gtaaaaaatg
    13321 ctcctgttat tgattcaaat gtcaatacac aaaaatctac tcctgaaatg gatttcacaa
    13381 ccacggaaaa aggagtcagg aattctttta atgaatttta taattattac gataataata
    13441 taaatggtta cagtaaagca gctaagtcat actcagctta ttaatttctc ttaggagtct
    13501 atcaccatga tgcgatcgct cttttccgga gtttccggtc tgaaaaatca ccagacacgt
    13561 atggatgtta taggtaacaa catctctaac gtaaacacct atggttttaa aaccagccgt
    13621 gtcagctttc aggatatgct ttcacaaact atttctggcg cagctcgtcc agaagaggaa
    13681 cgcggaggtg taaaccccaa gcaggttggc cttggaatga cagttgcctc tat
//
LOCUS       DAOTGZ010000055        13529 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_3064
            NODE_5447_length_13529_cov_4.586092, whole genome shotgun sequence.
ACCESSION   DAOTGZ010000055 DAOTGZ010000000
VERSION     DAOTGZ010000055.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868088
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 13529)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 13529)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:22:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,785
            CDSs (total)                      :: 2,751
            Genes (coding)                    :: 2,732
            CDSs (with protein)               :: 2,732
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 12 of 19
            Pseudo Genes (internal stop)      :: 5 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13529
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5447_length_13529_cov_4.586092"
                     /isolate="AMR_MDS_3064"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(108..1391)
                     /locus_tag="P5123_05360"
     CDS             complement(108..1391)
                     /locus_tag="P5123_05360"
                     /inference="COORDINATES: protein motif:HMM:NF012873.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="condensation domain-containing protein"
                     /protein_id="HRX15718.1"
                     /translation="MKRETSAYERVMFFYSDKSPMYFAFCLKIEGNLTDDRMKDALAK
                     TAKVYPLSAVRVEKDEQKRQFITTEDVPDFPLVLKDNFQGDWKDEVVLALQGHFDNST
                     GPMARFVLLRSGKLNHIIAVFHHAVCDGAGALLFLEEMLKFLADPLRKALVPDENNWA
                     PMLHKVISPDNLNKIKKFDLPPFATDKSYTKFEVQEKPYEPFPTLPFELQTVSFTKDE
                     TSKLIDVAKKNGVTVHAYAGALILQCFAEEFGEKEGFTRTIQSPINFRPQLVDGSEKM
                     FGLFNGLVTAECDCSPGVKTEEIARTIKSTFHSEIDSLKPLAGYYNFMTYFLEGIDDP
                     ELFYENRKEGGTPMNYDFSFSNLGRVGLESKYGEYYLKEIYGPVFSAAKGERVIGAIT
                     FDGKLFMTLIYDSACFDHQTGKRIWSKIRDRIMAL"
     gene            complement(1467..2819)
                     /locus_tag="P5123_05365"
     CDS             complement(1467..2819)
                     /locus_tag="P5123_05365"
                     /inference="COORDINATES: protein motif:HMM:TIGR00797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0042910 - xenobiotic transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006855 - xenobiotic transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MATE family efflux transporter"
                     /protein_id="HRX15719.1"
                     /translation="MFYKKDEFFLPRMVALAAPILLQNLLSSSLNFIDVFMIGRLGDT
                     AVAAAGGANQFFFIFLMLVFGIASGSAIFTAQYWGRKDIVNIRSIMGIGVTLTMGISI
                     LFTAVTAIFPDKVMALFSGDPEVIILGAGYIRIIAWSFIVMAMSISYAVVLRSTENVI
                     YPMMASMTGILLNTGLNYLLIFGNLGFPKMGVLGAAWATLFSRIVEMLIIISITYTKK
                     LPAAAGLSDLFRFNFQQVKAYLSKSVPVVLQSVGWAGGYSVYSMIYGHISTESLAAFS
                     IAGSVERICLIFYTGVGSACSIMVGNRIGAGEEKRARDYAGNFLFIGVIVSFVISILL
                     FVLRHPIVGIYNLSEVSQEYMLGILLVMSAIMWAKACNIIFHMGVFKAGGDTFFSMCV
                     DVGGVWLVGIPIALITGFYFGFPVHIIVAFLTIEEIVKMSVGFKRFLSGRWLNNLVDE
                     "
     gene            complement(3013..3456)
                     /locus_tag="P5123_05370"
     CDS             complement(3013..3456)
                     /locus_tag="P5123_05370"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRX15720.1"
                     /translation="MSYCKLLLLSIIFTVALNGASNLKPEDVQGSWKLRYKDGFGYEF
                     RLKEGYLAYIAVYIKGQLLVFKGVYNIDDGNILRVNISEMKEISNPSAIENRNGFVQT
                     SSSVFRFKIISKSEKSLIIEPKDIRIDGRSSGGYFEPRIELKSLK"
     gene            3864..4874
                     /locus_tag="P5123_05375"
     CDS             3864..4874
                     /locus_tag="P5123_05375"
                     /inference="COORDINATES: protein motif:HMM:NF012515.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenosine kinase"
                     /protein_id="HRX15721.1"
                     /translation="MHSSQFKYDLIGVGSPIVDLLAKINENFLLNIGGGKGGMQLVDD
                     SIMDKIITEIDKSTITVSPGGSAANTIFAASHLGLRTAFVGKLGRDENSRFYESEFRR
                     AGGSRSRFKHTYHNSTARCLSLITPDAERTMRTNLAAASELDVSDINSHDFQECRHVH
                     LEGYLMYNPELFIHILKTVKQSGCTISMDLGSYEVVNSSRLELTELISKYVDMVFANE
                     DEAEALFGKKDHEKNLDSLGDLCETAAIKIGPDGSLIKSGKDFVLVKPEPASARIDTT
                     GAGDLWASGFLYARLKGYDLATSGRAASYMGARAVEKTGTAFNSEEWQYIQNGIKDIL
                     SS"
     gene            complement(4875..5972)
                     /locus_tag="P5123_05380"
     CDS             complement(4875..5972)
                     /locus_tag="P5123_05380"
                     /inference="COORDINATES: protein motif:HMM:NF013283.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="BamA/TamA family outer membrane protein"
                     /protein_id="HRX15722.1"
                     /translation="MRFFRRIMFVIIAILIITNISAQEDRDSLVALPFVAYIPDTSLM
                     IGATAVYVVRPEDRTEADIPDTYQVVGFYTLKKQIQFAFIASNFLSHGFVNNVFTTSF
                     SKFNSVYYIDGMSDDQGEDYIPISYSFEDKFRLRLLRYVYAGPMFHYKSYRVDEYEEN
                     GYVDNSTIYGVKKSRVSAPGINVVFDNRDDTFYPTRGIFTEIIYLQNSEVVGATENSQ
                     CFEINAATFFNLYSKRYILGFHGRSVIQGGDVPYDMYAGIGGDKILRGYLNDRYKGRN
                     MYALQTELRFPFFFWDNTRFLKRLSAVVFVGAGSVSNDVTKLTTTKLHYAAGAGLRFA
                     LSDREKINLRLDFTRNREGNSEVYFQIMEAF"
     gene            6245..8764
                     /locus_tag="P5123_05385"
     CDS             6245..8764
                     /locus_tag="P5123_05385"
                     /inference="COORDINATES: protein motif:HMM:NF012711.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HRX15723.1"
                     /translation="MNSLYEFYKEICDKYANCMLFDELLTYGDSFKLALSRAAYLQKQ
                     GFKKGDVIGICAETSKEWCITYMAITMGGMIVLPLDTNLDSKEYPQMLKAVNTKAVFA
                     SEAQIGKFKKLKKYNTDFTKHVTDGKRFKQIAIKRDFPASYVFTSGTTGNPKIVVLSH
                     LNIFKTSIAASTWLDLNKDDKFLCILPLFHVYAFLANFIGPFSSGSSFIFLQSLKGPD
                     IVRTLSEHQFTVFPAAPQLWELFMDSILNKARAQSVLKYRALLFFLYTTPLFKAIGLG
                     FIPKKVFTPIRKIFGLKIRFFVSGGAPLKKRYFKFYSNMGLPIIEGYGLTETTGPITI
                     SHVKRNISGSVGPAMGNNEIRIKNKNSDGIGEIWLRGDSVMKEYYKNPSMTAEVFDKD
                     GFFNSGDLGKVDRKGYLFITGREKNVIVLDSGKNVYPEELEAFYKKSPEISEISVFGH
                     KIDNRETVYAVIVPFQKDKNSYQRIKSEINKLNRGLPSYKTISHFALSFDPLPVNSAR
                     KILVGEIITCLKRNAYMTAENDSVLHENEIIPQNDTEKKILAILIKKLGVKELYSSQT
                     LSDFDIDSIGIVDLTVYLEEKISVSINIDTLRSLGTINEVITYIATLPKSDSNNLDDQ
                     ILRSTISTRHISFFNPINELLGLTVRLTAKFFWNFRINNRENINADNVIYYSNHSSNL
                     DFITTWCYMSYRQRRKIFVIGKKEVSFLKYVFPGINIIFVERGGDIVPAMKASADLLR
                     KGKSLYIFPEGTRSKGEELGEFKTGAAYLAKNLDIKLIPLAIKGAAKILPAGKKWPRF
                     FKSESLTVTAGEKIDPNNYKTVQQLNKAMLKKMADLLKSDN"
     gene            8807..9973
                     /locus_tag="P5123_05390"
     CDS             8807..9973
                     /locus_tag="P5123_05390"
                     /inference="COORDINATES: protein motif:HMM:NF013347.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRX15724.1"
                     /translation="MNEQQLKRALKRHIVAEKHKIYVQCAPGFEEEMSEEIAEIIDDC
                     KTKITEGGIELKAGLDDIYKLNLISQCASRVLLRITRFYANTFTMLFENCLKTRWELY
                     LKKKTELIFSITVKKSKLYHTARIEEEMRKAISTHFDNNGYSDQSLDHHAKQMIHVRV
                     IKDIFYISLDATGEPLYKRGYKEHINNAPLRENIAASILRRVNLKKHDLLIDPMCGSG
                     TFSLEAMHMSSDVPPGIKRSFTFEIWPAFKTNHYDYLKKNLKKNGFSLAIMASDIDPR
                     SEKILLHNTKNAFCDEFSYKTEDFFNLHQSNFGNSRGILVLNPPFGKRFILNNQTSFY
                     NKIIQHIQSAFTNHDCALIIPANCISKIKLPDCEKSRLKLGGYETFLIIIKREN"
     gene            10052..11122
                     /locus_tag="P5123_05395"
     CDS             10052..11122
                     /locus_tag="P5123_05395"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRX15725.1"
                     /translation="MKRNNSIISSTIRQYSFMAGLLISASVAIFAVLLFFTYGKIETA
                     ASLAAFDNGYRFTELLRQSSSLNELKQKASSSDSIYGFTLFKRSRDDRFGLPVFSTEK
                     IITELDSQNIISDSDPSIFEKAFEKTVSDKKIYSDKDKYYTIIYFPFDTDKERYIARV
                     LVAVDNIAMIKINSSRHFIRAIKLLLTISVLAFAVFFFLSILFGIKIRNKFVKINKDV
                     SEAIANREKISDTNVEMVPLANSINSLIDDLLNQKKAIDKLKLLNDFDEIFKKGVEFL
                     KIKQYEAAKHIFHTLILVKPDSFGSVFNLGVIYAKQKDYPQSLSMFQSALSINPDDKL
                     SLQYCEKISKLAGAPLTNEKCS"
     gene            11106..12920
                     /gene="uvrC"
                     /locus_tag="P5123_05400"
     CDS             11106..12920
                     /gene="uvrC"
                     /locus_tag="P5123_05400"
                     /inference="COORDINATES: protein motif:HMM:TIGR00194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009380 - excinuclease repair complex
                     [Evidence IEA];
                     GO_function: GO:0009381 - excinuclease ABC activity
                     [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrC"
                     /protein_id="HRX15726.1"
                     /translation="MKNAHNLINDKLKQLTTEPGVYLMKNADNEIIYVGKARSLKNRV
                     SSYFHDSVDDIKTRTLVKQINDFEIIVTGTEIEALLLEDTLIKKHRPRFNIRLKDDKR
                     YPYIAITYSEDYPRLIFTRNLTNKKNRYYGPYTDAQAARNVVKAANRIFRLKTCSRKL
                     PLGPNERPCLNYQIRQCSGVCRNKISRDEYLDLVKNAELFLKGRIDPVINKLNETMNS
                     YSAQTRFEKAAEIRDIIFDIQKISERQNIMVPQNHDADYIAMDIFQEEAIVLFFEFRK
                     GILTGRKIRLYENSSFSTNASILRTFILDHYNNNDIPHFIITQERIEDSKILSDYFSS
                     KTGKKTELTIPKSQHDKGAFLMMRKNIDIIIAEKMAQDHYSDKNRYLINLKNILSLPE
                     IPIHMVCFDISNLQGTNAVASMASFKNGDPDKAGYRHFTIRGYEQANDPGMIHEAVAR
                     FIQNIINNDWEEPDLIVIDGGPTQLTRAIEARDALGLKTPIISIAKRHEELFTAPDLP
                     SIRLSHDSPELKIIQRLRDATHDFGVSHHRKLRSKSAVASELDNITGIGPKKRSALLK
                     HFKSVDAIRKAEDKQLMDVKEITAKDAKVIMEYFSKQK"
     gene            complement(13044..>13529)
                     /locus_tag="P5123_05405"
     CDS             complement(13044..>13529)
                     /locus_tag="P5123_05405"
                     /inference="COORDINATES: protein motif:HMM:NF026067.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4465 domain-containing protein"
                     /protein_id="HRX15727.1"
                     /translation="QYSVYSDSGNDGSEKFAIISSFVKETLISFEKPSNIIGFYINNT
                     TYVYHALKYGEDGSGYVTKSFGGNDGNDKDYLKLVIIGFDDEGVEIGQVEFFLADYRF
                     DDNSKDYIIRDWTWVDFSVFREPVKEIAFELVSTDSGDYGMNTPAYFAFDDLVYEVKP
                     E"
BASE COUNT         4659 a         2776 c         2236 g         3858 t
ORIGIN      
        1 tgtcgttgac cacatggtac ggattggttc cgttgacttt ttttcttttt tcataacagt
       61 ctcctttatc tttatgattt tatgcggtaa aatcaataat gtataactca taaagccatt
      121 attcgatccc tgatttttga ccaaatacgc ttaccggttt gatgatcaaa acaggcagaa
      181 tcataaataa gagtcatgaa cagcttccca tcgaaagtaa tagcgccaat tacacgctca
      241 cctttggcag cactgaaaac aggtccgtat atctccttaa gataatattc accgtatttg
      301 ctttccaatc cgacgcgccc aagattcgag aaggaaaaat catagttcat gggagtcccg
      361 ccctctttcc tgttttcata aaacagttcc ggatcgtcga ttccttcaag aaagtaggtc
      421 ataaagttat agtaaccggc taatggttta agactatcta tttctgaatg aaatgtactc
      481 tttatagtgc gtgctatctc ttcagttttc actccgggcg aacagtcaca ttcagctgtt
      541 acaagaccgt taaagaggcc aaacatcttt tctgaaccat caacaagctg cggacgaaaa
      601 ttaattgggc tttgaatcgt acgcgtaaaa ccctccttct ctccaaactc ctccgcaaaa
      661 cattgcaaaa tcagagctcc ggcataagca tgcaccgtaa ctccattctt ttttgcgaca
      721 tcaattaatt ttgaagtctc atctttagta aacgataccg tctgcaactc aaacggtaat
      781 gtaggaaacg gctcataagg cttttcttga acttcgaatt ttgtatagct cttgtctgtg
      841 gcaaatggtg gcagatcaaa cttttttatt ttattcaggt tatcaggact aataacctta
      901 tgcagcattg gagcccagtt attttcatca ggtactaaag cttttcttaa cggatctgcc
      961 agaaacttca acatctcttc cagaaaaagc agagctccag cgccatcgca cacagcatga
     1021 tgaaaaactg cgattatatg atttaatttt cccgacctta ataaaacaaa tctggccatg
     1081 ggaccagttg aattatcaaa atgtccctgt aaggccaata caacctcatc cttccaatca
     1141 ccctgaaaat tatcttttaa gacaagagga aaatcaggta catcttctgt agtaataaat
     1201 tgacgttttt gttcatcctt ttcgacccta actgctgata aagggtacac tttagctgtt
     1261 tttgccagcg catctttcat tctatcgtct gtaaggttcc cctctatttt taagcaaaat
     1321 gcgaaataca ttgggctctt gtcactataa aagaacatta ccctttcgta cgcggaagtt
     1381 tctcttttca taatattact cctaaatatc aatttgtgat ctaaatattt gcaaaaaata
     1441 aatttgaggg atcctcaaat aatttatcac tcatcgacca gattattaag ccaacgccct
     1501 gaaagaaacc gcttaaaccc cacactcatc ttgacaatct cttcaatagt taaaaatgcc
     1561 actataatgt gcacaggaaa accaaagtaa aacccggtta taagtgctat tggaatacca
     1621 accagccaga ctcctccgac atcaacacac atactaaaga aagtgtctcc acccgcttta
     1681 aaaacaccca tgtggaagat aatgttacat gctttagccc acattatagc cgacataact
     1741 agtaaaatac cgagcatata ctcttgactt acttcactta aattgtatat gccgacaatt
     1801 ggatggcgta aaacaaaaag caatattgat ataacaaatg aaacgataac accgataaaa
     1861 agaaaattgc ccgcgtaatc tctcgccctt ttctcttcac ccgcaccaat gcggttaccg
     1921 accataatcg aacaggcact accaacacca gtataaaaaa tcaggcagat tctttcaaca
     1981 gaacctgcta tgctaaaagc agcaagagac tctgtactaa tatgtccata aatcatcgaa
     2041 tatacactgt aaccgccggc ccaccccacg ctctgtaaaa caacaggaac cgacttggaa
     2101 agataagcct ttacctgttg aaaattgaaa cgaaacagat ccgacaaacc agctgcagca
     2161 ggaagctttt ttgtataagt aatcgatatt ataattaaca tctcgacaat acgggaaaaa
     2221 agagtagccc atgccgctcc aagcacaccc atctttggaa atcccaaatt accaaaaata
     2281 agaagatagt ttaatcctgt atttaacaaa attccggtca tacttgccat catcggatag
     2341 ataacatttt ctgtactgcg taaaactacc gcgtaagaaa ttgacatagc cataacaata
     2401 aagctccaag caattattct tatgtatccg gctccaagga ttatcacttc aggatcccca
     2461 gaaaaaagag ccattacctt atccggaaaa atggctgtta cagcagtaaa caagattgaa
     2521 atacccattg ttaaagttac acctataccc ataatagaac ggatattcac tatgtctttt
     2581 cgtccccagt actgtgctgt aaaaattgca gatccgcttg ctataccaaa taccagcatt
     2641 aaaaaaataa agaaaaactg gtttgcgccg ccggccgccg ccacggcagt atcgccaaga
     2701 cgtccgatca tgaatacatc aataaaattt aaagatgacg aaagaagatt ttgaagaagt
     2761 atgggggccg caagggcaac cattctgggc aaaaagaact cgtccttttt ataaaacata
     2821 acagcctccc gtaaggtcta acgatattta gaagaaaaat cacaaacgga aggaatctgg
     2881 tcaagatatt attttcaaag ggtgtctgct gcaaccgaat ttacagattt tttttaacgc
     2941 acacaacaac cggcaacaga aaagacttga gagggggcta atataaattt ttatagcagc
     3001 atcaataatt aactacttta aagattttaa ctcgatacga ggctcgaaat atccaccgga
     3061 acttcttccg tcaatacgaa tatccttggg ttcaattatt agactttttt ccgatttaga
     3121 gataatttta aagcggaaaa ccgaagatga agtttgcaca aatccattac ggttttctat
     3181 agccgatggg tttgatatct ctttcatttc actaatattt actcgcaaaa tattaccatc
     3241 atcgatatta tagactccct taaaaaccaa caactgcccc tttatgtaaa cagctatata
     3301 tgcaagatat ccctctttaa gacgaaattc atatccgaaa ccatccttat atcgaagctt
     3361 ccaagacccc tgtacatctt caggttttaa atttgaagca ccatttagcg cgacagtaaa
     3421 aattattgat aaaagcagca atttacaata actcatttta tcctccgata caattcataa
     3481 taaacactta tacatcaata tataaggtaa tcacttacac atttcaatct taacaaattt
     3541 gataattgag tcgtatcgat aattatattc tgagaaaacc atgtccatct tttttctttt
     3601 ttttattcct ttactctata tcttatattt ttacaaataa gattaccaaa atggagaagt
     3661 agttaaaaga ttgtattatt gaattccgcg ttcacaataa atagataacc gtaactataa
     3721 cctaaaattt tccaaaacca gccggatttt cttgtgatac atgctataac aattctgtat
     3781 gagtgatatt gttgataaaa accttgacac tgattatagc attgcctatt gaaatcacta
     3841 ccagctaaat agaggagtta tatatgcatt cttcacaatt taaatatgac cttattggtg
     3901 taggttctcc gattgttgat ctactggcta aaattaacga aaatttttta ttaaacatag
     3961 gcggcggaaa aggaggaatg caacttgttg atgacagcat aatggataag atcatcaccg
     4021 aaattgataa atccactatt acagtttcac ctggtggctc cgctgcgaat acaatatttg
     4081 ccgccagtca cctggggcta agaactgctt ttgtaggcaa actcggccgt gacgaaaact
     4141 cacgttttta tgaaagcgaa ttcaggcgag ccggtggcag tagatcccga tttaaacata
     4201 cctatcacaa ctctaccgcc agatgccttt cactaataac ccccgatgca gaaagaacca
     4261 tgcgaaccaa cctcgccgcg gcgtcagaac ttgatgtatc tgacattaac agtcatgatt
     4321 ttcaagaatg ccgccatgtc catcttgaag gttaccttat gtataaccct gaactattca
     4381 tacacatatt gaaaacagta aaacaatcag gctgcactat cagtatggat ctaggctcat
     4441 atgaagttgt taattcatca agacttgagt taaccgaatt gatttcaaaa tatgttgata
     4501 tggtatttgc aaatgaagat gaggccgaag cacttttcgg aaaaaaagat catgaaaaaa
     4561 accttgattc ccttggcgat ctttgtgaaa cagcagcgat aaagataggg ccggatggtt
     4621 ctctaataaa atcagggaaa gatttcgtct tagtgaagcc tgaaccggcc tctgccagaa
     4681 tagatactac cggagcagga gacctatggg cttctggatt tttatacgcc cgattaaaag
     4741 gctatgacct tgctacaagt ggcagggcag cctcatatat gggagccaga gccgtagaaa
     4801 agacaggcac tgcatttaac tccgaagaat ggcaatatat tcaaaacgga ataaaagaca
     4861 ttttgagctc ttaatcaaaa tgcttccatt atctgaaaat atacttccga attaccttct
     4921 ctatttcttg taaaatcaag tctgagatta attttttctc tatcgcttaa tgcaaatcgc
     4981 aatcctgcgc cagccgcata atgcagtttt gtcgtagtaa gtttagtcac atcatttgaa
     5041 accgaacctg ctcctacaaa aacaactgcg gaaagccttt ttaaaaatct tgtattatcc
     5101 caaaaaaaga acggaaatct taactcggtc tgcaaagcat acatattacg ccccttatat
     5161 cgatcattaa gatatccgcg aagtatttta tcgcctccga taccggcgta catatcataa
     5221 ggaacatccc caccctgaat cacagagcga ccatgaaacc caagtatata acgtttggaa
     5281 tacagattaa aaaaagtcgc agcatttatc tcgaaacatt gagaattttc ggtagctcct
     5341 acaacttcac tattttgcaa atagatgatt tctgtaaaaa ttccccgcgt tggatagaag
     5401 gtgtcatcac ggttatcaaa caccacgttt attcctggag cagaaacgcg gctttttttt
     5461 actccataga tagtactatt atccacatag ccgttttcct catattcgtc aactcgatac
     5521 gatttataat gaaacatagg tcccgcataa acataccgaa gcaaacgcaa tctgaattta
     5581 tcttcaaaag aataacttat tggtatataa tcttcaccct gatcatcact cataccgtcg
     5641 atataataaa cagaattaaa tttgctgaaa cttgtagtaa aaacattatt aacaaaacca
     5701 tgtgataaaa agtttgacgc aataaacgcg aactgaatct gcttttttaa agtatagaac
     5761 cccacaacct gatatgtatc aggaatatcg gcttctgtgc gatcctctgg acgtacgaca
     5821 tacaccgcag ttgcccctat cataagactt gtgtctggga tataagctac aaagggcagt
     5881 gcaacaagag aatcacggtc ttcttgagca gaaatatttg taataattaa tatcgcgata
     5941 ataacaaaca ttattcttcg aaaaaaacgc atagtctggc tctcctgcat agatcgaccc
     6001 gtcggcctgt acataacata ttttgattta cttaataata catgacaaag cgacagtata
     6061 actcatatcc tgtcaatata acttaatttt tatttaataa ccaaagtcat gatttcttta
     6121 catattatta acaaccacta agcgaaaagt gcccccagct ttcaacccat agaatgaaaa
     6181 tatattgata ttattaaggc ttatcccata tcatgttaca aagcttgaat tacaggagct
     6241 gccaatgaac tctctttacg aattttataa agaaatatgt gacaaatacg caaattgcat
     6301 gctttttgac gaactgctta cctacgggga ctcttttaaa cttgctcttt caagagcagc
     6361 gtaccttcaa aagcagggat tcaagaaagg tgacgtaata ggtatttgcg ccgagacttc
     6421 aaaagaatgg tgtattacct atatggcaat cacaatgggt ggcatgattg tactgccact
     6481 ggacacaaat ctggattcca aagagtatcc acaaatgctc aaagcagtaa acaccaaagc
     6541 agtttttgca tcagaggcac aaataggcaa attcaaaaag ttaaaaaaat acaatactga
     6601 ttttacaaaa catgtcaccg acggcaaaag atttaaacaa attgccatta aaagagattt
     6661 ccctgcttca tacgtattta cgtccggaac gacaggaaat ccaaaaatag ttgtactctc
     6721 tcatcttaat atcttcaaga catcaatagc tgcaagtact tggcttgatc taaataaaga
     6781 cgacaagttt ctctgtattt taccgctttt tcatgtttat gcatttcttg ccaactttat
     6841 agggcctttc agctctggct cttcgtttat ctttctacaa tcacttaagg gaccagatat
     6901 agtacgcacc ctttccgaac accaattcac agttttccca gctgctccac aactttggga
     6961 actttttatg gactcgatac ttaataaagc tagagcacaa tctgttttaa aatatcgagc
     7021 cctacttttt tttctttata ccaccccact ttttaaggct atagggctcg gttttatacc
     7081 aaaaaaagta tttactccaa ttagaaaaat ttttggatta aaaattagat ttttcgtaag
     7141 cggaggagcc cctctaaaaa aacgctactt taagttttat tcaaatatgg gcttacctat
     7201 aattgaagga tacggtctaa ccgaaactac cggtccgata acaatatcgc acgttaaacg
     7261 gaatatttca ggctccgtag gccctgcaat gggtaacaac gaaataagaa taaaaaacaa
     7321 aaattctgac ggtatcggtg aaatatggtt aagaggtgac tccgttatga aggagtatta
     7381 taaaaacccc tccatgactg ccgaagtttt tgataaagat gggtttttca actcaggaga
     7441 cctcgggaaa gttgacagaa agggatatct ttttataacc ggacgtgaaa aaaacgtaat
     7501 agtactcgac tcaggtaaaa acgtatatcc tgaagaactc gaagcttttt acaaaaaatc
     7561 accagagatt tcagagatat cagtttttgg acataaaatt gataaccgcg aaaccgttta
     7621 tgcggtaata gtacctttcc aaaaagacaa aaacagttat caacggataa aatctgaaat
     7681 caacaagctc aaccgaggtt tgccatccta taagactatc agccattttg cactctcttt
     7741 tgaccctcta ccggtaaatt cggctcgtaa aatacttgta ggcgaaatca taacctgttt
     7801 gaaaagaaac gcatatatga cagccgaaaa cgactctgtt ttgcacgaaa acgaaatcat
     7861 tccacaaaac gatactgaaa agaaaatttt agccatacta attaaaaaac tcggagtaaa
     7921 agagctctac tcttcacaga cactctcgga ttttgatatc gactcaatcg gtattgttga
     7981 tctgacagta tatcttgaag aaaagatatc agtttcgatc aatattgaca ctctcagatc
     8041 tttaggcaca ataaacgaag taataaccta tatcgcaacg cttccaaaaa gtgacagcaa
     8101 taatcttgat gaccaaatac tcagaagcac tatctcgaca aggcatatct ctttttttaa
     8161 cccaattaac gagctgttgg gtttaacagt acgattaacc gctaaatttt tctggaattt
     8221 cagaataaat aatcgtgaaa atattaacgc cgacaacgta atctattatt ctaatcacag
     8281 ttccaacctg gactttatta caacttggtg ttatatgtct tacagacaaa gaagaaagat
     8341 atttgtaatt ggaaaaaaag aagtaagttt tcttaaatat gtctttccgg gaataaacat
     8401 tatcttcgtc gaacgcggag gtgatatagt accggcaatg aaggccagcg cagatctgct
     8461 acgcaaaggt aagtctcttt atatttttcc agaaggaacc cgttctaaag gcgaagagtt
     8521 gggagaattt aaaaccggag cggcatacct tgcgaaaaat cttgatatca aactaattcc
     8581 attggcgatc aagggtgcgg caaaaatttt acctgcaggc aaaaaatggc cccgattttt
     8641 taaatcggaa tcattaacgg taacagccgg agaaaaaatt gatccgaata attacaagac
     8701 cgtacagcaa cttaataaag caatgcttaa aaaaatggct gatcttttga aatctgataa
     8761 ttgattacag ctacctaatt acactggaac cgcaaatgga gcataaatga atgaacaaca
     8821 actaaaaaga gcattaaagc gccatattgt tgcggaaaag cataaaattt atgttcaatg
     8881 tgcccccgga tttgaagaag agatgtcaga agagatagcg gagataattg atgattgcaa
     8941 aacaaaaata actgagggag gtatagaact caaggccggt cttgatgata tttacaaact
     9001 gaatcttatt agccaatgtg cttcaagagt tcttctgcga attacacgtt tttatgctaa
     9061 caccttcacc atgttgtttg aaaactgtct aaaaacaaga tgggagctct atctcaaaaa
     9121 aaagactgaa ctaatatttt ctataactgt aaaaaagtca aagctgtatc acacagcaag
     9181 aattgaagaa gagatgcgaa aggcaatatc cactcatttt gacaataacg gttattctga
     9241 ccaatcatta gatcatcacg caaaacagat gatacatgtt cgcgtaatca aagatatttt
     9301 ttatatcagc ctagatgcaa ctggggaacc tctttacaag cgaggttaca aagaacatat
     9361 aaataacgca ccccttagag aaaacattgc agcctccata ttaagaagag tcaatctaaa
     9421 aaagcatgat ctactgatag accccatgtg cggttccgga actttttctc tcgaagcaat
     9481 gcatatgagt tccgatgttc cccccggtat aaaacgctca tttacctttg agatatggcc
     9541 tgcattcaag accaatcact atgattacct taagaaaaac ttaaaaaaaa acggattcag
     9601 tcttgctata atggcatcag atattgatcc ccgctccgaa aaaattttat tgcacaatac
     9661 caaaaatgcg ttttgcgatg agttcagcta caagaccgaa gattttttta atctgcacca
     9721 gagtaatttc ggcaattctc gtggtatact ggttttaaat cctccatttg gtaaaagatt
     9781 cattctaaat aaccaaacat cattttataa caagattata caacatattc agagtgcctt
     9841 tactaaccat gattgtgcct tgattattcc agcaaactgt atctccaaaa taaaacttcc
     9901 cgattgtgaa aaaagcaggc ttaaactggg cggatacgaa acttttctga taataataaa
     9961 aagagaaaat taaaactgat cattattgtt gacaactata atcaggattt ttatatttat
    10021 ttctgttata taattcaaac aggttcaaaa tttgaaacgt aataattcca ttatatcgtc
    10081 aaccatcagg caatactctt ttatggctgg tttgctaatt tctgcttctg tagcgatttt
    10141 tgcggttctt ctctttttca catatggaaa gatagaaact gccgcttctt tagctgcctt
    10201 tgataacggc taccgcttta ctgaattatt gcgtcaatca agctcattaa acgaattaaa
    10261 acaaaaagct tcctcttcag atagcatata cggatttaca ctttttaaac gctctagaga
    10321 cgaccgtttt ggtttaccgg tgttttcaac agaaaaaatt ataaccgaac ttgacagcca
    10381 aaatatcata agtgactctg atccttcaat tttcgaaaag gcatttgaaa agacagtttc
    10441 tgataaaaag atatattcag ataaggataa gtattataca attatctact ttccttttga
    10501 caccgacaaa gagagatata ttgccagagt tcttgtagct gtagataata tagccatgat
    10561 caagataaac tcttcccgcc attttatcag ggcaataaag ttactcctta cgatatcggt
    10621 cttggctttt gctgtattct tttttctttc gattttgttt ggcatcaaaa ttcgtaataa
    10681 attcgtaaaa ataaataaag atgtatcgga ggccattgca aacagggaaa agatctcaga
    10741 cactaacgtg gaaatggtgc ctcttgcaaa cagcatcaac tctctgatag atgacttgct
    10801 taaccagaaa aaggctattg acaagttaaa gcttctaaat gattttgatg agattttcaa
    10861 aaaaggagtc gaatttctga aaattaaaca atacgaagca gccaaacata tttttcacac
    10921 actaatactg gttaaaccgg atagttttgg ttccgttttc aatttaggtg tcatatatgc
    10981 caaacagaaa gattatcccc aatcactttc gatgtttcaa tccgcattat ccattaatcc
    11041 agatgacaaa ctgtcacttc aatactgtga aaaaatcagc aaactagctg gcgccccgtt
    11101 aactaatgaa aaatgctcat aacctgataa atgacaagct aaaacaactt accaccgaac
    11161 ccggcgttta cctaatgaaa aatgccgata acgaaataat atatgttggt aaagcccgat
    11221 cacttaaaaa cagagtcagc tcttattttc atgattcggt agatgacatt aaaactcgca
    11281 ctcttgttaa acagattaat gatttcgaaa taattgtcac aggaacagaa attgaggctc
    11341 ttcttctcga agacacatta attaaaaaac accgtccccg ttttaatata agactaaaag
    11401 acgataaacg ctatccatat atcgcgatta cctatagtga agattatcca cgtcttattt
    11461 ttacacgcaa cctgacaaac aaaaaaaacc gttattacgg tccctacacc gacgcacaag
    11521 ccgcaagaaa cgttgtgaaa gccgctaacc gtatttttcg tctaaagaca tgttctcgaa
    11581 aacttccact tggaccaaat gagcgcccat gtttaaacta ccagatacgc caatgcagtg
    11641 gagtttgtcg caataaaata agcagggatg aatatcttga tcttgtgaaa aacgcagaac
    11701 tttttttaaa aggaagaatt gatcctgtaa ttaataaact aaacgaaacc atgaacagct
    11761 attcggctca aactcgtttc gaaaaagctg ccgaaattcg tgatatcatc ttcgatatac
    11821 aaaaaatctc tgaacgccaa aacatcatgg taccgcaaaa ccacgacgct gactatatag
    11881 ctatggacat ttttcaagag gaagcaattg ttctcttctt tgaatttcgt aaaggaatat
    11941 taacgggtcg caaaatccgc ctttatgaaa attcatcgtt ttcaactaat gcctctattt
    12001 taagaacttt tatactggat cattacaata acaatgatat accccatttc attattactc
    12061 aggaaagaat tgaagatagc aaaatattaa gtgattattt ttcaagcaaa accggcaaga
    12121 aaacagaatt gacgattcca aaatcacagc atgacaaagg cgcatttttg atgatgcgta
    12181 aaaacataga cataataatt gcagaaaaaa tggctcagga tcactactcg gataaaaaca
    12241 ggtatcttat caatttaaaa aacatcttat ctctccccga aattccgatt cacatggtat
    12301 gttttgacat atctaatttg caggggacga acgcagtagc atcaatggcc agttttaaaa
    12361 atggtgaccc tgacaaagcg ggatatcgtc attttaccat aaggggatat gaacaggcaa
    12421 acgatcccgg aatgattcac gaagcagttg cacgctttat acagaatatt attaataatg
    12481 attgggaaga acctgatctt attgtaattg atggtggacc aacgcagcta acacgtgcaa
    12541 tagaagccag agacgctctt ggtctcaaaa cccccattat ctctatcgct aaaaggcatg
    12601 aagaactatt tacggctcca gatctaccgt cgataagact ctcgcatgac tcccctgaac
    12661 tcaaaataat tcaaaggctc cgtgacgcga cacacgattt tggagtgtca catcatagaa
    12721 agctcagatc aaaatcagct gtagcgtccg aactggacaa tatcactgga attgggccca
    12781 aaaagagaag cgctctttta aaacatttca agtcagttga cgcaatccga aaagccgaag
    12841 ataagcagtt aatggatgtg aaagagatta cagcaaagga tgcgaaggta ataatggaat
    12901 atttttcaaa acaaaaatag attctatact tgtactcaca attattgata ctattctatt
    12961 aaccggcaag aagtattgaa tgccggttaa tagaaaacat ttttttaatc acaatacctt
    13021 aacaaacaac aatcatacaa catctattcc ggttttacct cataaaccag atcatcaaat
    13081 gcaaaatatg cgggagtatt cataccgtaa tcaccggaat cggtcgaaac aagttcgaaa
    13141 gctatctctt tgacaggctc tctgaacact gagaagtcaa cccatgtcca atcacgaatt
    13201 atataatcct tgctattgtc gtcgaaacga taatcggcga ggaaaaactc aacttgacca
    13261 atctctacac cttcatcatc aaatccaatg ataacaagtt tcaaataatc tttgtcattc
    13321 ccatcgtttc caccgaatga ttttgttacg taaccagatc catcttctcc atatttgagc
    13381 gcatgatata cataggtggt attgtttata taaaacccta taatatttga tggtttctca
    13441 aaagaaatta gagtctcttt aacaaaggaa gagataattg caaatttttc tgatccatca
    13501 ttaccgctat ccgaatacac actatattg
//
LOCUS       DAOTGZ010000056        13447 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_3064
            NODE_5490_length_13447_cov_4.721625, whole genome shotgun sequence.
ACCESSION   DAOTGZ010000056 DAOTGZ010000000
VERSION     DAOTGZ010000056.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868088
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 13447)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 13447)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:22:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,785
            CDSs (total)                      :: 2,751
            Genes (coding)                    :: 2,732
            CDSs (with protein)               :: 2,732
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 12 of 19
            Pseudo Genes (internal stop)      :: 5 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13447
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5490_length_13447_cov_4.721625"
                     /isolate="AMR_MDS_3064"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1777
                     /locus_tag="P5123_05410"
     CDS             <1..1777
                     /locus_tag="P5123_05410"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HRX15728.1"
                     /translation="SENEELAYKQALKESEARFKALHDASCGGIALHDNGKIIICNQG
                     LSDMTGYSQEELLGMNGLLLIMEDDRPFVKEQIAASTEKPYETRCVKKNGDILNLRIR
                     ARNVKYNGRTVRATEFRDITDMKNARKNIDDALEYLQMILRTTRDGFWIIESDGRLSE
                     VNDAYCKMTGYNRDELLGLTISDIDALEDPEKTAKRIERIMTQGADLFQTKHKRKDGT
                     VFDIEVSSSLLSGHNKMICFGRDITKRKEIEQQLFELNSNFLTLLNNTNDFIYYKGED
                     RRLKFCSQAMADITGFKSWRDMIGKHDIEIFPKDVAKIYIEEENDIYSKGLPVINKID
                     PYYDRNKKRGWVQTSKWPVFDNDKKTVTGLFGISRDITEIKEIEQKLNRELEEKNMLL
                     HEINHRIKNNIGSIEALLSIQASSNNSTETKAALSEAASRVKSMQSLYENMLISNKYD
                     VVNADHYLGGIAKSVTDFYSDAVNITLITEIDKLDLKVNLAFPLGIIINELITNAAKY
                     AFTETGLGTIFISLKEYKDTDSERSKAEIILKDNGKGLPDDFLPDQAEGFGLMLVNML
                     CRQTESRLSIYSQNGTVVKILVPLS"
     gene            1949..2539
                     /locus_tag="P5123_05415"
     CDS             1949..2539
                     /locus_tag="P5123_05415"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRX15729.1"
                     /translation="MKKLIVLISFFVIVNTGFAQTETIDPNNKDLWWINIGFGKGGMF
                     GGDLDNAKNSEHDVNNLSLLASSSFYSPQIGFPITVRTAASAEQVMMFTSDIEYIYDY
                     GVLIGQMHKTRWGRIVYSAGISTVKGMYIYGFEDASNETIWKYKSYETIGMPLQVELF
                     FTPIKYIGVGITGYGNLNLKRSFWGAAVCFQFGVLN"
     gene            complement(2564..3244)
                     /locus_tag="P5123_05420"
     CDS             complement(2564..3244)
                     /locus_tag="P5123_05420"
                     /inference="COORDINATES: protein motif:HMM:NF013382.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HRX15730.1"
                     /translation="MERDKITERFDIVADQYDSQRRYFIPCYDDYYKTSLYFLASIRK
                     DFASVLDLGAGTGLLSKYLYSRFPNANFTLVDLSKKMIDVAKERFSGMNNFRYIISDY
                     SKELPDGEFDLIASALSIHHLTEEDKINLYNNIYKKLNKNGVFINLDQFNAQSETLNS
                     HYNSWWYKYIENSGVRNDERDLWLKRRELDRENSVAKTIDMLNKAGFDDAECIYSYMK
                     FGVVLAIK"
     gene            3729..4916
                     /locus_tag="P5123_05425"
     CDS             3729..4916
                     /locus_tag="P5123_05425"
                     /inference="COORDINATES: protein motif:HMM:NF012377.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 5 protein"
                     /protein_id="HRX15731.1"
                     /translation="MKKKFIICTPVFLSFVLAAILVTGFPLSSGIAAKKGFIHASGRN
                     LIDPEGKKILLKGIAFGNEVWSNPTTPPSNHHSAKDYKVIKDLGFNSVRFYLNYRLFE
                     DDSKPYKYKKSGFDWIDKNIAWAKKNGIYLVLNMHVPHGGFHSNAETTDLWTKDGNEE
                     RFLALWKKIAEHCKDENTIAGYSILNEPAVYGGIGKWSEFTKQTVAAIRQTDKNHLII
                     IERMQCNIKSPRNADWNENVNGMMNFPPVKDSNIMYEFHFYKPFPLTHQGASWVPALS
                     GVKAPYPGPFKDWDGSTKMGDKKYLYKEFKPYIDFSKKKNSPVYLGEFGVIKQGFDDG
                     KSALAWINDIIDICDKNRINFNYHTYHEVYFGLYQNEPYKLPNNLNKPLAEYFKTRLS
                     TIN"
     gene            complement(5002..6567)
                     /locus_tag="P5123_05430"
     CDS             complement(5002..6567)
                     /locus_tag="P5123_05430"
                     /inference="COORDINATES: protein motif:HMM:NF012245.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein"
                     /protein_id="HRX15732.1"
                     /translation="MKKQFGNSNIDQYENEVDIFANVMRRNNVRILKAFVSIVLMANI
                     ATVAIKITGASSAYLTYRHIIVELALISLTLTVSYVVSKKTQNALVASYLFITGIVLS
                     VWIFLYVIYGASELFAVHYVALALSIFYFDKKIIIYTLFLILLSQTTLFLLRPGLVPT
                     GPSSNLIIRYLIYLWTGIGAAVGSGSTKELLGLAVSKHSEAQKNVSKMRTLARALADS
                     VGVLRNHVSDQEGVTLEMETISQSQASSLTQISAVLEELAANSESINNISKSLFSDIN
                     QTVGSVEQFKEINDSVIENSDDIIKTLEEISSYSEKNSLHIKMTKQKSEILKTKSDEM
                     SGFVQVINDIADQVTLLSLNASIEAARAGESGKGFAVVADEISKLAEATTNNAKEINN
                     IIYENQKQIDESTTLIDESSVMTVKLNEAILKITGSFHDAGKRMRIIGDKIRAIRQIN
                     SNIYETSSSIDHSIQQQESGISESSETISNISINAQKIVSISNAIRVANNTLNDMSSQ
                     MDILANSMLSADE"
     gene            6861..7484
                     /locus_tag="P5123_05435"
     CDS             6861..7484
                     /locus_tag="P5123_05435"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRX15733.1"
                     /translation="MKHLLYLITITFLLSITHPISAQTDSIKDDSDFLFWINYGFGIH
                     QNYGGIISKSDYCENETFGIAALASSTAYLPSVDMIFTIRALGTSNVFLDYTDDSEQC
                     SDFGIMIGKIKKSRWTYVSFSTGLGYVYGGYSGKPKALLYEGEDNSDPEYEYNVTEYK
                     TAGIPMQIELMLTPLNCFGFGIVVFANLNIERPFYGALFCIQSGRLF"
     gene            complement(7563..8234)
                     /locus_tag="P5123_05440"
     CDS             complement(7563..8234)
                     /locus_tag="P5123_05440"
                     /inference="COORDINATES: protein motif:HMM:TIGR01065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin III family protein"
                     /protein_id="HRX15734.1"
                     /translation="MQTDKLSKDKSLHVTDEVFNSSSHMAAAVFSVLGAAFLITRSAA
                     TGQVWHIVSFSIYGVSLFAVFLFSTLHHGVNSNQRTEMILRNCDYIAIFPLIAGSYTP
                     ICLILLRDSYIGWTIFGVIWATAVLGITLRAGFKSLPEWVLNTLYITMGWLGAFVAIP
                     IYRHIGISGFMLFLGGGLFYSIGFVIYNIEKPNPVPGKFGFHEIWHIFVILGALAHFL
                     MMMLL"
     gene            complement(8259..8645)
                     /locus_tag="P5123_05445"
     CDS             complement(8259..8645)
                     /locus_tag="P5123_05445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018525596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HRX15735.1"
                     /translation="MGEKAILCVDDEAIIRLALIQELKKNFKDRFLYEQAPDALIAMD
                     TLEELSHEGVRVVLIISDWLMPGINGDEFLENVHEKYPEIRAIMITGHADGKSIERVR
                     KNESVLAVMEKPWSSLELVSVIEKEF"
     gene            complement(8721..10433)
                     /locus_tag="P5123_05450"
     CDS             complement(8721..10433)
                     /locus_tag="P5123_05450"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HRX15736.1"
                     /translation="MLLNKKKRLVDGSLRNSLMLSVAVIFFIQLLFLTVLFVFTRINI
                     NRRASVTADEIAEILQEPMYNVDDAGVKKIADALMAAGRIEGLRIVSNATGQVYNSLG
                     NDTDSLIVALQKREVLEQGLNLGNIYLKFSDSEFWETVYVNSMITFILVLTVSGVNIW
                     VNRTFILKKVKTVFASVGRDIKKIRAGSYDTSIELSGYDDVDSIIASINDMSQTIVMQ
                     NRELREINETLELRVKQRTVELERSVNELEKMQNRVVESGKLSVLGQLAAGIAHEFNT
                     PLGAIISTTETLIAYVDKDLFALYDFRSTLSEEESSLYQYLLEIGFEKGRSFNPEFID
                     RQTIKQLSEKLNKHGVSDARQIAVLLTEMQTHVNIDDYIEMLKGGRASEIVSFVANAV
                     AARRMIAVIEESARRAAQVVSAFKQYLGSGLSATIEVVRVEEDLNRVLVLLQNILKKG
                     VSISTSFSGLAIRGVSDKLNQVWINIIRNAVQAMDGKGKLQLITEKSGDMGIVKIIDS
                     GKGIPEHNQNQIFDPFFTTRESGEGMGLGLDICRRIVNDLGGTIDFHSKPGHTEFIVA
                     LPLA"
     gene            complement(10420..11208)
                     /locus_tag="P5123_05455"
     CDS             complement(10420..11208)
                     /locus_tag="P5123_05455"
                     /inference="COORDINATES: protein motif:HMM:NF012707.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter substrate-binding domain-containing
                     protein"
                     /protein_id="HRX15737.1"
                     /translation="MMHRLFFLILLLLFSLQMHAAETLHIAVINLEPFLTDEGKGKTP
                     GGIAVDYWRDYIAPRMNVSVKFSGPYPIRRAEYMLKNGEVDAVMHLTKIPEREKDFLF
                     PETHLTGIKSCLVVPVDSSITKVLKTEDMYGWTILFIDSAYVPEMLLHRRINIEKVSG
                     TDFRHIQLNMLLASRGEAMLDINQVSLLFYLRNRGYLDRVRIITLPVKPQKVYTIFSK
                     TAKGMDLKRRFDKVNSKGLKKGVFERMTQDYLKRSGLANRNAVK"
     gene            11528..12013
                     /locus_tag="P5123_05460"
     CDS             11528..12013
                     /locus_tag="P5123_05460"
                     /inference="COORDINATES: protein motif:HMM:NF024854.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HRX15738.1"
                     /translation="MKTEQIRKLRSILRLLNRTLFSCFDETSSCCGVSTSECHVLMEL
                     GIEEGVSLTNIAEKLQINPGNMSRTIEKMVQNGLIERRTQPENRRSVSLFLTNAGRAQ
                     ANEINDSYDKLFKELLSLIDESKHEMIIESIDLLTKASISIKDENEICCKKDNHGLII
                     G"
     gene            12019..13026
                     /gene="hgcA"
                     /locus_tag="P5123_05465"
     CDS             12019..13026
                     /gene="hgcA"
                     /locus_tag="P5123_05465"
                     /inference="COORDINATES: protein motif:HMM:NF040863.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0042192 - methylmercury biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mercury methylation corrinoid protein HgcA"
                     /protein_id="HRX15739.1"
                     /translation="MKTDASFISGYMNTGNQSIPIIKSQLSPRDHLGSVAVRLGYSRG
                     NYMVEPGLYALGKPGAFSPVIVTANYKLTVDIVRRDLNRQNLWILVIDTAGINVWCAA
                     GKGTFGTQNIAKWILKSGLNDIVKHRRLILPQLGATGVAAHTLQKQTGWIIQYGPVRS
                     RDLPAFLKGEETPPMRTVSFNFFERLILIPVEIYTGLKFMLITLSATFFYEVLLYLVR
                     GKFHFTTIASVMVGFIAGTIFVPLLLPVLPSRYFFVKGFFAAVSLWIPFVLMLNPDLF
                     TAISSLFIAGSISSFLAFNFTGATTFTSLAGVRYEIRAALPFAMVLMISGLITIFFVR
                     S"
     gene            13145..>13447
                     /locus_tag="P5123_05470"
     CDS             13145..>13447
                     /locus_tag="P5123_05470"
                     /inference="COORDINATES: protein motif:HMM:TIGR01909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C-GCAxxG-C-C family protein"
                     /protein_id="HRX15740.1"
                     /translation="MSRSEIGVNKFKEGFNCSQSVLFSFADRLGISEDNALKIMTGFG
                     AGMGRRQEICGAVSGGIAVLSMLYGRTGSDDKQKSEETYDKTRKLIEAFISNYGTVN"
BASE COUNT         4387 a         2653 c         2333 g         4074 t
ORIGIN      
        1 atccgagaac gaggaactcg cctacaaaca ggcattaaaa gagagtgaag cccgttttaa
       61 agccctgcac gatgcctcat gcggtggtat tgccctacac gataacggta aaattattat
      121 ctgcaaccaa ggactttcgg atatgaccgg ttattcacaa gaagagcttc taggaatgaa
      181 cggcctactg cttataatgg aagacgatcg ccctttcgtg aaagaacaaa tcgctgcttc
      241 aaccgaaaaa ccttatgaaa cgcgttgtgt gaaaaaaaac ggagatattc ttaaccttcg
      301 catacgtgca cgcaatgtta aatataatgg aagaacagtt cgagcaaccg aatttagaga
      361 tataacagac atgaaaaatg cacgtaaaaa cattgatgat gctctcgaat atcttcagat
      421 gattttacgc actacccgtg acggtttctg gattattgaa agcgacggcc gtttaagcga
      481 agttaacgat gcctactgca aaatgacggg ctataacagg gatgaattat taggtttaac
      541 gatttcggat attgacgcat tagaagatcc cgaaaagacg gcaaaacgga tagagagaat
      601 aatgacacag ggcgccgatc tttttcagac aaagcataag cgaaaagacg gcactgtttt
      661 tgatatcgag gtttcttctt ctttattaag cggacacaat aaaatgatct gctttggccg
      721 tgacattaca aaaagaaaag agatcgaaca acagcttttc gaactcaaca gcaacttctt
      781 aaccttgctt aacaatacca atgactttat atattataaa ggagaagatc ggcgtttgaa
      841 attttgcagc caagcaatgg cagatattac gggattcaaa agctggagag atatgatcgg
      901 caaacatgat attgaaatat ttccaaagga tgttgcaaaa atctatatcg aagaagagaa
      961 cgacatctac tcaaagggtt tgccagtaat aaacaagatt gacccctatt acgatcgcaa
     1021 taaaaagaga ggctgggttc aaacaagtaa atggccggtt tttgataacg ataaaaaaac
     1081 cgtcaccggc ctttttggaa taagtcgaga cataacggaa attaaagaga tagaacaaaa
     1141 acttaaccga gagcttgaag aaaaaaacat gctgttacat gaaataaacc acagaataaa
     1201 aaacaatatc ggctcaattg aagctctgtt gtccatacag gcaagcagta acaactcaac
     1261 cgagactaaa gctgcattat cagaagcggc ctcaagagta aaaagcatgc aaagccttta
     1321 cgaaaacatg cttataagca ataaatatga cgtcgtaaac gccgaccatt atcttggtgg
     1381 aattgccaaa tctgtaacag atttttattc tgacgctgta aatattactc ttattaccga
     1441 aatagataag cttgatctga aagttaatct tgcttttcca ttgggaataa tcattaacga
     1501 gcttattact aacgctgcaa aatacgcatt tactgaaaca ggattaggta ctatctttat
     1561 ttcacttaaa gaatataagg ataccgattc agaacgatca aaagctgaaa taatattaaa
     1621 agataacggt aaaggtttac cggatgattt tttaccggat caagctgaag gttttggtct
     1681 tatgcttgtc aacatgcttt gccgtcaaac cgaatctaga ctttcgattt actctcaaaa
     1741 cggaaccgtt gtgaaaattc ttgttcctct aagctaaaca agtctacaac tagtatttag
     1801 ttaaaaccat tattaattta attgtttaca attaaattac ggtctattaa ttgtgtttta
     1861 atgtttaatt aatactaata ttgttattat gcgttaatat atataatata ttattaatat
     1921 tattaatgca ctgcaaaagg acatatatat gaaaaaacta atagtactaa tatcgttttt
     1981 cgttattgtc aataccggtt ttgctcaaac cgagacaata gatccaaaca acaaagatct
     2041 atggtggatc aacatcggtt tcggcaaagg aggtatgttt ggcggagatc ttgataacgc
     2101 aaaaaattca gaacacgatg taaataattt gtcactatta gcaagcagtt ctttttacag
     2161 tccacagatt ggctttccaa ttacagtacg tactgcagca agtgccgaac aggtgatgat
     2221 gtttacttcc gatatcgaat atatttatga ttatggtgta cttataggac aaatgcataa
     2281 aacaaggtgg ggcaggattg tctattcagc tggtataagt acagttaaag ggatgtatat
     2341 ttacggcttt gaagatgcat caaatgaaac aatatggaaa tataaatcgt atgagacaat
     2401 aggtatgccc ctacaggtcg aattattttt tactcctatc aaatacatcg gagttggtat
     2461 tacaggttat ggaaatctaa atttaaaacg atcattttgg ggtgctgcag tctgttttca
     2521 gtttggagtt ttaaattagg tgcaacgtaa ttattacata attttatttt atagccagaa
     2581 caactccaaa tttcatataa ctgtaaatac attcagcatc atcaaaacct gctttattta
     2641 gcatatcaat tgttttagca acactatttt cacgatcaag ctcacgtcgt tttaaccaca
     2701 aatctcgctc atcgtttctt accccactat tttcgatata cttgtaccac caactattat
     2761 agtggctgtt taaagtttca gattgggcgt taaactgatc aagattgata aaaactccgt
     2821 ttttattaag tttcttgtaa atattgttat acagatttat tttatcttct tctgtaaggt
     2881 gatgtatgga taaagccgat gcaatcagat caaactctcc gtccggtagt tcttttgaat
     2941 aatctgatat gatataccgg aaattattca tgccagaaaa tctttctttt gcgacatcaa
     3001 tcattttttt cgacaggtct acaagtgtaa aatttgcgtt tggaaaacgg ctgtaaagat
     3061 attttgataa tagccctgta ccagcgccaa gatctaacac agaagcaaaa tcttttctaa
     3121 tacttgcaag aaaatatagg ctggttttgt aatagtcatc atagcaggga ataaagtatc
     3181 tacgttgact atcgtattga tctgcaacga tatcaaatcg ttctgtaatt ttgtctcgtt
     3241 ccatattaag gttacctgcc tggctatttt cttatgacat gatggtattc ctgctattct
     3301 atccgaattc tgcacatttt tatcaactct ttttatcaga atccaatcgt aatgcacctt
     3361 ttatatagca catattacct taatacaggc ctcattttta taccgttttt tattaaattt
     3421 aatcttgctg ccaataacga aaatacagat aacaatttta tgacttataa acctaccggt
     3481 atggatttgc cccccaaaaa aatatgggct ctatttctga ttcagactat tgcggcaacc
     3541 gttcttacgt ttctccaata tttaatgaaa tttttacaga caagacactc tgacatttta
     3601 aagattaaat cacttgaaaa tctgaaaaat tacctgatga aaaatgaaac cgcttatata
     3661 aaaaatagtt gcaaactcat tcagataatt gaaatttaat ctgctccaac agagagagag
     3721 agaataatat gaaaaaaaaa tttattatat gtactccagt atttttatca tttgttttag
     3781 ccgccattct tgtaacagga tttcctttaa gtagcggtat tgcagccaaa aaaggcttta
     3841 ttcatgcttc aggtcgcaac ctaattgatc ctgaaggcaa aaaaatacta ctcaaaggca
     3901 tagcttttgg aaacgaagtc tggtctaacc ccacaacccc tccatcaaat catcactcag
     3961 ccaaagacta caaagttatc aaggatcttg gttttaactc tgtgaggttt tatttaaact
     4021 acaggctttt tgaagatgac agcaaaccct ataaatataa aaaaagcggc tttgactgga
     4081 tagacaaaaa catagcctgg gctaaaaaga acgggatata tcttgtgtta aacatgcatg
     4141 ttccccatgg cgggtttcac tctaatgcag aaactacaga cttatggact aaagatggca
     4201 acgaggagcg ttttctggct ttatggaaaa aaattgctga gcattgcaaa gacgaaaaca
     4261 caatagcagg atacagtatc ttaaatgaac cggccgttta tggcggaatt ggtaaatggt
     4321 ccgaattcac aaaacagact gttgctgcca taaggcaaac agacaagaat catcttatca
     4381 taattgagcg tatgcaatgc aacataaaat caccaagaaa tgccgactgg aatgaaaatg
     4441 ttaatggaat gatgaacttt ccaccagtta aagacagcaa tatcatgtac gagtttcact
     4501 tttacaagcc gtttccgtta acacaccagg gagcgtcctg ggtacctgcc cttagtggcg
     4561 tcaaggcacc atatcccggc ccctttaaag actgggacgg ttcaacaaaa atgggggaca
     4621 aaaaatattt atataaagaa tttaaaccct atatcgattt ttctaaaaag aaaaatagtc
     4681 ctgtatatct tggtgaattt ggggttatta agcagggctt tgatgatggc aaaagcgccc
     4741 ttgcctggat aaatgatata attgatatat gtgacaaaaa caggataaat tttaactatc
     4801 acacttatca cgaggtttac tttggtcttt atcaaaacga gccgtataaa ctacctaaca
     4861 atttgaataa acctttggca gaatacttta aaaccaggct aagcactata aattagatta
     4921 cttttaacaa aaccgggaga cctttttaaa taaagccttc cggtttttta aatttaaaat
     4981 ttaatctctc ttaacgtttc actactcatc agcagacaac atactgttcg caagaatatc
     5041 catctgtgac gacatatcat taagagtatt gttagccacg cgtattgcat ttgatataga
     5101 tactatcttt tgagcgttta ttgaaatgtt tgaaatagtt tcactggatt cactgatacc
     5161 cgactcttgc tgttgaattg aatgatctat ggaagagctg gtctcgtata tattcgaatt
     5221 aatttgccgt attgctctta tcttgtcgcc gattatacgc atacgcttgc ctgcgtcatg
     5281 aaaacttccg gttattttta atatggcttc gtttagttta accgtcataa ctgatgattc
     5341 gtcaatcaat gtagtgctct catctatctg cttctgattt tcatagataa tattattaat
     5401 ctctttggca ttatttgtgg tagcctcggc cagttttgaa atttcatctg ctaccaccgc
     5461 aaaacccttc cctgattcac ccgcccttgc agcctcaatc gaagcgttca acgatagcaa
     5521 agtcacctga tcggcaatat cgtttattac ctgcacaaat cccgacatct catcactctt
     5581 ggttttaagt atttcagact tttgcttggt cattttaata tgcagcgagt tcttttcaga
     5641 atagctggat atctcttcaa gagttttgat aatatcatcc gaattttcga ttacagaatc
     5701 gtttatctct ttgaactgtt caacggaacc tactgtctga ttaatatcac taaatagtga
     5761 ctttgatata ttattaattg actcggaatt cgcggcaagc tcttcgagaa cagcagaaat
     5821 ctgtgttagt gatgaggcct gactttgtga aatggtctcc atttctagag taacaccctc
     5881 ttgatctgaa acatgatttc ttagaactcc tactgaatca gcgagagcac gtgccaatgt
     5941 tctcattttt gacacatttt tttgtgcctc tgaatgtttc gatacggcta gcccaagcaa
     6001 ctccttggta gaccccgatc ctacagcagc tccaatacca gtccataaat agatcagata
     6061 tcggataatc agattacttg acggaccagt tggaacaagc ccaggtcgta acaaaaaaag
     6121 agtggtctgt gataacagaa ttaaaaacag tgtatagata attatttttt tatcaaaata
     6181 gaagatactc aatgccaacg caacatagtg aaccgcaaat aattccgagg caccgtaaat
     6241 aacataaaga aaaatccata cggatagcac aataccagtt ataaagagat aacttgctac
     6301 aagcgcattt tgagtttttt tagagacaac ataagagaca gtcagagtca aagatatcaa
     6361 agcaagttcg actataatat gtcgatatgt taaataggcg gacgaagcgc ctgttatttt
     6421 tatcgcaaca gttgcgatat ttgccattag cacaatactt acaaatgcct ttaaaatacg
     6481 aacattatta cgcctcatta catttgcaaa gatatccact tcgttttcat attgatcaat
     6541 attactatta ccaaactgtt ttttcatatt aaacatcctg attttttgga aaaattcttt
     6601 taaaacatgt aaaaatcaat aaaaacgcaa ataacagata tgactaataa aatatgaatt
     6661 gaaattcatt agtatttttg atataataaa aatcaataaa attttgttat taataatttt
     6721 aatcggtttt aataattcaa attgttaaac agttcggtat tgaaaatacc gaatatcata
     6781 acagctgtct tatctttatc gatttttatt caaaatcctt aatttcacat gtttagaaat
     6841 taaactcatg aggtaaatag atgaaacacc tgctatattt gatcactata acatttttgc
     6901 tttccataac ccaccccata tctgctcaaa cagattcaat taaagatgat agtgattttt
     6961 tgttctggat aaactatggg ttcggcatcc accaaaatta tggcggaatt atttcaaaat
     7021 cggattactg tgaaaatgaa acattcggta ttgcagcatt ggcaagttca acagcatact
     7081 tgccctctgt cgatatgatt ttcacaatac gagctttggg tacctccaat gtttttttgg
     7141 attatacgga tgattcagaa caatgcagcg attttggcat aatgataggc aaaataaaga
     7201 aaagccgttg gacatacgtt tctttctcta ccggacttgg atatgtttat ggaggctata
     7261 gcggaaaacc aaaggcatta ttatacgaag gtgaagataa cagcgaccct gaatacgaat
     7321 ataatgttac cgaatacaaa acagctggta ttcccatgca aatagaactc atgctaacac
     7381 cattaaattg cttcggtttt ggtattgttg tttttgcaaa tttaaatatc gaaaggcctt
     7441 tttatggagc cttattctgc atacagagcg gaagactgtt ttgatgaaca atcattaaat
     7501 aactgtcacg gctcaaatgt aaggtttgac tacagaaaca aggcagtaac aaactatgca
     7561 cattacagaa gcatcatcat caagaaatga gccagcgcac caaggataac gaagatatgc
     7621 catatttcat gaaagccaaa cttgcctgga acagggtttg gtttttcgat attatatata
     7681 acaaaaccaa tgctgtagaa tagcccccct cctaagaaca acataaaacc cgaaatacca
     7741 atgtgtctgt atatcgggat cgctacaaaa gctcccaacc aacccatagt aatatacaac
     7801 gtattaagaa cccactcagg taagctctta aaaccagctc gaagtgttat acccaaaaca
     7861 gctgttgccc atattacgcc gaaaatggtc caaccgatat aactgtcacg aagtaaaatt
     7921 aggcaaatcg gagtataaga gccggcaata aggggaaata tggcgatata atcacaatta
     7981 cgtaaaatca tctcggttcg ttggttggaa ttgaccccat gatgcaatgt actaaaaaga
     8041 aaaactgcaa acagagaaac tccataaata gaaaaactga cgatatgcca cacctgcccc
     8101 gttgcagccg agcgagtaat taaaaatgcc gcgcccaata ccgaaaaaac ggccgctgcc
     8161 atatgtgacg aagagttgaa gacctcgtct gttacatgaa ggcttttatc tttacttaat
     8221 ttatcagttt gcatcctccc cccgattaag aaatgttttt agaactcttt ttcaataaca
     8281 cttacaagct ctaaactgct ccatggtttt tccataacag ctaaaacaga ttcgtttttg
     8341 cgaactcgtt ctatggattt tccatctgca tgaccggtta tcattatggc tcttatttcc
     8401 ggatattttt catgcacatt ttctaaaaac tcgtctccat taataccagg cataagccaa
     8461 tcagatatta tcaatacgac tctaactcct tcatgcgaaa gttcttccag tgtatccatt
     8521 gctataagtg cgtcaggtgc ctgttcgtat aaaaatctgt ctttgaaatt ttttttaagt
     8581 tcctgaatta atgcaagtcg tataattgct tcatcatcaa cgcataaaat tgccttctca
     8641 cccatacctt accctcataa atatttccac attttttata caagatatac cctattaaac
     8701 cataatctat gcatatgttt ctatgccaaa ggcagagcca caatgaactc ggtatgccca
     8761 ggctttgaat gaaaatctat ggttccgccc agatcattaa ctattcgcct acaaatatca
     8821 agccccaacc ccattccctc accactttca cgggtcgtaa aaaatggatc aaatatttga
     8881 ttctgattat gttcagggat accctttcca gagtcgatta ttttgactat acccatatca
     8941 ccgctttttt ctgtgattaa ttgcagcttg cctttgccgt ccatcgcctg aactgcatta
     9001 cgaataatat ttatccatac ctgattaagt ttatccgata ctccacgaat tgcaagtcca
     9061 ctaaaagagg tactaatact aacacctttt ttcaaaatat tttgcaaaag aaccagaact
     9121 cgatttaaat cttcctctac cctaacaact tcaatagttg ctgataagcc tgaaccaaga
     9181 tactgtttaa aagctgatac aacttgtgct gcgcgacgcg cggattcttc tattacggca
     9241 atcattctgc gagccgcaac ggcattggcc acaaaactta caatttcaga agcgcgcccg
     9301 cctttaagca tttcaatgta atcatcaata ttcacatgag tttgcatttc cgttaacaaa
     9361 acagcaattt gcctggcatc ggacacacca tgtttgttta acttttcaga taattgcttt
     9421 attgtttgcc tatcgataaa ctccggatta aatgaacgcc ccttttcaaa accaatttcc
     9481 aacaaatatt gataaagact cgactcttct tcactaagag tggagcgaaa atcataaaga
     9541 gcgaagagat ctttgtccac ataagctatc aaagtctcag ttgtagaaat tatggcgcct
     9601 aacggcgtat taaactcgtg agcaattcca gccgcaagtt gaccaagcac cgaaagtttt
     9661 cctgattcaa caaccctgtt ttgcatcttt tccagctcgt ttaccgatct ttccagttcc
     9721 actgttctct gtttcactct gagctcaagt gtctcgttaa tctcgcgcag ttccctgttt
     9781 tgcataacaa tcgtctgcga catatcattg attgatgcga taatcgaatc cacatcatca
     9841 tagccgctca attcaatgga tgtatcgtac gagcctgctc ttatcttttt tatatccctt
     9901 ccaaccgagg caaaaacagt cttcaccttt tttagaataa atgtcctatt cacccatata
     9961 ttaactccag acactgtcaa aacaagaata aaggtaatca tggagtttac ataaactgtc
    10021 tcccaaaact cactatcact aaactttaaa tatatatttc ccaggtttaa accctgttct
    10081 aatacttctc gtttttgaag agcaactatc agagagtcag tatcattacc aagagaatta
    10141 taaacttgtc ctgtcgcatt tgacactata cgcaatccct caattcgtcc agcagccatt
    10201 aacgcatcag caattttttt aacaccagca tcatcaacat tatacatcgg ttcttgcaat
    10261 atttcggcaa tttcatcagc agtcacacta gcccgtctat ttatatttat acgagtaaaa
    10321 acaaaaagta ccgtcaaaaa tagaagctgt ataaaaaaaa ttacagctac cgacagcatt
    10381 agcgaattac gcaggcttcc atctacaaga cgtttcttct tatttaacag catttctgtt
    10441 tgccaaaccc gatcttttta aataatcttg tgtcattctt tcgaaaaccc ccttcttcag
    10501 gcctttactg ttaactttgt caaatctcct tttaagatcc attcccttag cagtttttga
    10561 aaaaatagta tataccttct gaggttttac cggtaatgtt ataatccgta cacgatccaa
    10621 ataacctctg tttcgtaaat agaaaagcaa agagacctga ttgatatcaa gcatagcttc
    10681 acccctacta gcaagcaaca tattaagctg gatatgccta aagtcggttc cagatacctt
    10741 ttcaatatta atgcgtctgt ggagaagcat ttcgggaaca taggcagaat cgatgaaaag
    10801 tatcgtccat ccatacatat cctctgtttt gagaaccttt gtaatcgaac tatctaccgg
    10861 aaccacgagg caactcttga tacctgtcaa atgtgtctcg ggaaaaagaa agtccttctc
    10921 gcgctctggt atttttgtaa ggtgcattac agcatctact tcaccgttct taagcatata
    10981 ctcggcacgt ctgatagggt atggcccgga aaattttaca gatacattca ttcgtggtgc
    11041 aatataatcc cgccaatagt ctacagcgat gcctccagga gtttttcctt ttccctcatc
    11101 tgttaaaaac ggctcaagat ttattacggc aatatgcaag gtctctgcag catgcatttg
    11161 aagagaaaat aataaaagca ggataagaaa aaacaggcga tgcatcatga ttaaacccct
    11221 tattttttat gaccgtctca ttagctcata ccgcatctcc gttgagccat taacacgaaa
    11281 acatattaat cattagctca taccacatct tccgctaaac cattaacacg aaaacatatt
    11341 aatcaaatat agccctaaga ttacatctcc cccgccgtcg gcgggggaaa ccaactatta
    11401 gttaaagcaa atatagccct aaacagcata attttcaaac aaaagtaatg gataatcgaa
    11461 agaggccaaa aatcacttga ctttatttgt attgtacaac atttgttatt aacaacatag
    11521 gaaacttatg aaaacagaac agataagaaa actgcgctcg atactgcgct tattaaaccg
    11581 tacactattt tcctgttttg acgaaacctc ttcatgctgc ggtgtttcta cctcggaatg
    11641 tcatgttcta atggagctgg ggattgagga aggagtgtct ctgacaaata ttgctgagaa
    11701 acttcagatt aatcccggca atatgagtcg tacgatagaa aaaatggttc aaaacggcct
    11761 tattgaacgt cgaactcaac ccgaaaacag aagaagcgtt tccctttttc tgacaaatgc
    11821 aggaagagct caagctaacg aaatcaatga ttcatatgac aagcttttca aagagctact
    11881 atcacttatt gatgaatcaa aacatgaaat gattatcgaa tcaatagatt tactgacaaa
    11941 agcatctatc tctatcaaag atgagaatga aatttgctgc aaaaaggata atcacggact
    12001 tattataggc tgaaacaaat gaaaacagat gcttcattta tttccggtta catgaatact
    12061 ggaaatcaga gtataccaat aataaagagt caattgtcgc caagggatca cctcggtagt
    12121 gtcgcagttc gtcttggata tagtcgcggc aactacatgg ttgaacctgg cctttacgcc
    12181 ttgggtaaac ccggcgcgtt ttcgcccgtt attgttacag ccaactataa attaacagtc
    12241 gacattgtac gccgcgacct taacagacag aacctctgga tactggttat cgataccgca
    12301 ggcattaatg tctggtgcgc ggccggcaag ggaacttttg gcacccagaa tatcgcaaaa
    12361 tggattttaa aaagcggcct taacgatata gtaaaacacc gcaggctcat cttgccacag
    12421 cttggagcaa cgggagtcgc ggcccatacc ttgcaaaaac agacgggttg gataattcaa
    12481 tacggaccgg tcagaagccg ggatctgcct gcttttttaa aaggtgagga gactcccccg
    12541 atgagaactg taagtttcaa tttttttgaa cgacttattc taatacctgt tgagatttat
    12601 accggattaa agttcatgtt gatcactcta tcggcaacat ttttttatga agtgttgctc
    12661 tatcttgtca ggggtaaatt tcattttact acgattgctt cagttatggt aggttttatt
    12721 gccggtacaa tttttgtacc acttctttta cctgtattgc catcgcgtta tttttttgtg
    12781 aagggttttt ttgcagcagt ctcgttatgg attccatttg ttttgatgct aaatcctgat
    12841 ctatttacgg ctatctcttc actttttatt gcaggttcga tcagttcgtt tttggctttt
    12901 aattttaccg gagcaacaac ctttacgtca cttgccggtg taagatacga gatacgagca
    12961 gcgctcccat ttgcgatggt tttaatgata tctggattaa taacaatttt ttttgtacga
    13021 tcctgattga ttatctgaaa tgaaaaacga gcacaagtaa ataggttttg atatttgata
    13081 attaataaag tagatgctaa accttgatgt tttatattta atcacttaaa cagttggagg
    13141 agcaatgagc agatcagaaa ttggtgtaaa taagttcaaa gaaggattta actgttcgca
    13201 atcggttctt ttcagttttg ctgaccgtct tggcatatct gaagataatg cattaaagat
    13261 aatgacaggt tttggagccg gaatgggacg ccgtcaggag atatgcggag cagttagtgg
    13321 cggtatcgcg gtgttgagta tgttgtacgg cagaactggt agtgatgata aacaaaaaag
    13381 tgaagagact tatgacaaaa cccgcaaact tatagaagca tttatctcta attatggtac
    13441 agtaaac
//
LOCUS       DAOTGZ010000057        13432 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_3064
            NODE_5493_length_13432_cov_4.748972, whole genome shotgun sequence.
ACCESSION   DAOTGZ010000057 DAOTGZ010000000
VERSION     DAOTGZ010000057.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868088
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 13432)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 13432)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:22:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,785
            CDSs (total)                      :: 2,751
            Genes (coding)                    :: 2,732
            CDSs (with protein)               :: 2,732
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 12 of 19
            Pseudo Genes (internal stop)      :: 5 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13432
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5493_length_13432_cov_4.748972"
                     /isolate="AMR_MDS_3064"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..336
                     /locus_tag="P5123_05475"
     CDS             <1..336
                     /locus_tag="P5123_05475"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024812.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HRX15741.1"
                     /translation="LFVAESDNQIIGWISLSPYRGGREGLLKTVEVSYFVDNDFQQRD
                     VGSKMLKKILEKAKNLEYETVVAIIFESNLGSKKLLQKNGFERWGYLPDVAQIDGKRF
                     SHGYYGLKL"
     gene            complement(346..1104)
                     /locus_tag="P5123_05480"
     CDS             complement(346..1104)
                     /locus_tag="P5123_05480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019028160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HRX15742.1"
                     /translation="MIKRLTVLIIPTILFFFITATHGEKNSNHGNKKNNHCVILLHGL
                     ARTESSMTKMEQSLITEGYHVINKGYPSRSKEIQPLSEETISIAISECAKFEPQKIHF
                     VTHSMGGILVRYYLDKNRIENLGRVVMLSPPNRGSETVDKLKDFFVFKWLNGPAGQQL
                     GTGSKSIPNQVGAPYYEVGIITGDRSINPILSLLIPGDDDGKVSIERAKLSGMKDFIV
                     VHNTHTFIMSDNDVIEQVIHFIQNGHFDKSANSK"
     gene            complement(1612..2382)
                     /locus_tag="P5123_05485"
     CDS             complement(1612..2382)
                     /locus_tag="P5123_05485"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRX15743.1"
                     /translation="MKKFSKSLLTFLTIILTSFLLFIITACDGDDDDKDSNDVDPKGK
                     WMAVLDDEIEAPSPDTVMAKAILLEVTAENSTMIFYDSATEQISGMKSTYTVDENTFQ
                     LTSTHMWLDRDPEEGITFTGTGWYEIPDEVETLNPEFTIDDDTMTIDKDGVKFSLIKT
                     EFSASSNLVGTWAMTGSSATFNSDGTFSYSHESQTGSGTWEASGTESGYIRQIITEMS
                     DDPDCSYYECLTPYVLVGNTLTLYYDFVMSTDYDYTKE"
     gene            complement(2641..3186)
                     /locus_tag="P5123_05490"
     CDS             complement(2641..3186)
                     /locus_tag="P5123_05490"
                     /inference="COORDINATES: protein motif:HMM:NF013073.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="C40 family peptidase"
                     /protein_id="HRX15744.1"
                     /translation="MLKKIIISITAVFAIFISGCEIVDDILSGESDDNSGNPGESEES
                     EEWSSVIDTDLESSLLRDDLILLAREQIGTPYLYGGATPSGFDCSGLIYYVYGEYDFT
                     TLPRTSRDMYSNGTSVGMDRAKLADIVAFYSPVSHVGMIISSTTFIHSPSTGKTVTEA
                     EFAGYWERQLAGVVTYIDDKR"
     gene            complement(3192..3608)
                     /locus_tag="P5123_05495"
     CDS             complement(3192..3608)
                     /locus_tag="P5123_05495"
                     /inference="COORDINATES: protein motif:HMM:NF024206.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ankyrin repeat domain-containing protein"
                     /protein_id="HRX15745.1"
                     /translation="MKKIALLTIIFCFYLGIAAQNSIIDTTRSGDHTTLSKILKAGKS
                     PDIPNRFNKTALMYACYDGDFESVKILIENGADINRQDNEGWTALFYAVSKNREDIAQ
                     YLIAKGANKQIKDISGKRPYDYAVADGNTQIADMVR"
     gene            3977..4660
                     /locus_tag="P5123_05500"
     CDS             3977..4660
                     /locus_tag="P5123_05500"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRX15746.1"
                     /translation="MSISQRKQQFIKKIVKLYSNSGLPIHYTSLAQEIGVSKWSSYEM
                     LIELEKLGFIERQYQNNRNKSGRSRTIFSPTEKALTLFETLSGEQDMPVDEKVWNNKT
                     SLLKIELDELKALPVGQSINSVIGKIETEEVKLFKMAYMFFIYLIIAFKSGTCISKEI
                     IEIIRSVSSPVSQYMMLSGATLENLLENNIVDSPEVMLPLVKRSINNISSIPSADLKK
                     LIEFFLDIV"
     gene            complement(4683..5039)
                     /locus_tag="P5123_05505"
     CDS             complement(4683..5039)
                     /locus_tag="P5123_05505"
                     /inference="COORDINATES: protein motif:HMM:NF036460.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5329 family protein"
                     /protein_id="HRX15747.1"
                     /translation="MKNILILMLFLTTSTLAEEATKSDMSEEQKIDYLIQCVINLKDA
                     QFVRNGKSYDSIAAGNHLKMKRKKAGTRIKTVNDFIEKLASHSLFSGKPYLIIYKSGR
                     EIDAETFYRQCLARLK"
     gene            5340..5864
                     /locus_tag="P5123_05510"
     CDS             5340..5864
                     /locus_tag="P5123_05510"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HRX15748.1"
                     /translation="MNIAKDKIGGFFKQEYKRMKGFVQSYISDCVDSESDDIIQDVMI
                     KLSSLADVTVPLDNLSSYVYRALKNRIIDIYRSDNKNKSVPIESNNVEVVSLSDDSLD
                     VSQIFEESQLRENLIAALGRLSESERTLIIMTEYEGKTVRYISEKTNEPVGTLLARKS
                     RAVNKLRYYMKQYI"
     gene            5891..6310
                     /locus_tag="P5123_05515"
     CDS             5891..6310
                     /locus_tag="P5123_05515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016836217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRX15749.1"
                     /translation="MNHFWKTKDGKPKVLHIIAGVSGGIIVACIFAFVFSFAVQYLWN
                     WLLTDLFGLSRIDLYQSFGLVILAKILFGGFGHGHHHKPYGDQDSQQTVAVTNCCGEC
                     SCYDDVKENWVSYSDFWQEEGRVAWENFKRKKDTTNK"
     gene            complement(6364..6720)
                     /locus_tag="P5123_05520"
     CDS             complement(6364..6720)
                     /locus_tag="P5123_05520"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRX15750.1"
                     /translation="MYKVLNLVNPLHYMQIKELPVLCLNPHTILSELNPARGELTHSD
                     LMLSRTLKGWALCKADENGPLVFGPGRFRFKQKPLPGRIDLADYSDETTLRIIIEDSS
                     ALLQEIIPDEIISEQA"
     gene            complement(6809..8164)
                     /locus_tag="P5123_05525"
     CDS             complement(6809..8164)
                     /locus_tag="P5123_05525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015757529.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="citrate/2-methylcitrate synthase"
                     /protein_id="HRX15751.1"
                     /translation="MSKKTDDFFSDLEQRTLNRNSINPDLNLKYRPKRGLRNPDGTGV
                     LAGLTQIGEVHGYVMDESEKTPVEGRLMYRGIDLRDIVAGFQTDNRFGFEETCYLLLF
                     GDLPNENQHLEFRDLLNSYRGLPENFTEDMILKAPSPNIMNKLARTVLSLYSYDDEAE
                     DRNLKNIIKQSIMLISRFPVLTAYAYHTKHHYYDNGTLHLRHIPEDLSTAEVFLSLLR
                     SDGNYTKLEAEILDLALVLHAEHGGGNNSAFTVRAITSSDTDTYSAIAAGIGSLKGYK
                     HGGANIKVLQMVDNIKANVKNWSNESELEDYLAKIIRKEAFDGNGLIYGMGHAIYTYS
                     DPRAILLKEKAQLLAVEKGHEEELNLYYNIEKLTPGLFHEIKQNEKIIAANVDMYSGF
                     VYQMLDIPDDIYTPLFAMSRISGWCAHRIEEIISGGRIIRPAYKCVQPKQQYVPIAER
                     Q"
     gene            8492..9406
                     /locus_tag="P5123_05530"
     CDS             8492..9406
                     /locus_tag="P5123_05530"
                     /EC_number="2.5.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014904095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016765 - transferase activity,
                     transferring alkyl or aryl (other than methyl) groups
                     [Evidence IEA];
                     GO_process: GO:0006535 - cysteine biosynthetic process
                     from serine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine synthase family protein"
                     /protein_id="HRX15752.1"
                     /translation="MVYGSILDTIGSSPCVKLNRLNPYPEVQMYAKLEGFNPTGSIKD
                     RIALKMVEQAEKEGTLNRKKIIIEPTSGNTGIGLAMIGAVKGYRVHIVMSEAVSIERR
                     RMIEAFGAKVILTDSSKGTDGAICEAHRIIESDPDSYFMPNQFSNHYNKMAHYKTTAV
                     EIWNDTEGRVTHFVSSLGTSGTLMGVGKGLKEKNPNIKIVEAHPEKGHYIQGLKNMEE
                     AIVPEIYDPSMVDCSVMVRTDVAFAAARQIVLKEGIFAGMSSGAALYAALEVAHKIRE
                     GVVVMIFPDRGEKYLSTDLFLNSGDNNE"
     gene            9399..>10051
                     /locus_tag="P5123_05535"
     CDS             9399..>10051
                     /locus_tag="P5123_05535"
                     /inference="COORDINATES: protein motif:HMM:NF013862.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="patatin-like phospholipase family protein"
                     /protein_id="HRX15753.1"
                     /translation="MSKEGYCLVLGGGGAKGVYHAGVWQALRELRVPISAVVGNSVGA
                     LVAGFIAQKNIKGLDSVMENLGLTDLVSIPEKLVIDGEFKLTRSNFKEFISFRKQLFE
                     KKGLDTSPLKKILNNHLDESKLRSLGIDLGVVTYSLSSFKPIEIFVDQMPEGTVLDYL
                     LASATLPGFSATEINEKKYIDGGVHDNIPFAMAKNRGYKRIIVSDISGLGIKRRPEIT
                     "
     assembly_gap    10052..10151
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <10152..10536
                     /locus_tag="P5123_05540"
     CDS             <10152..10536
                     /locus_tag="P5123_05540"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRX15754.1"
                     /translation="KMRCSKDLVYSLADCAASALIIERVKLYRFEDLIKEIELKAANV
                     DFRIKKLKKAITGRELRSLIRQIKKISKQGIGLSDIQNCPWFYYRIVSDIVPEPLRPV
                     LFSSITALYPETASCALFLKIIGKM"
     gene            complement(10591..>13432)
                     /locus_tag="P5123_05545"
     CDS             complement(10591..>13432)
                     /locus_tag="P5123_05545"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRX15755.1"
                     /translation="IIFTIFCTFLLCFHNLEAGHPYSLKWKSIDTENFQIIYNPDIEE
                     DAQRVANLAEHYLKHHNKTMKSDWRRTTIVLISESAEANGSVFYAPYKSHFYNTPGQF
                     AANEWFTALSVHEIRHMSQLNTINSGGRKITWYVFGDIGAAIYAVAFVPSWIMEGDAV
                     LAETTLTNGGRGRQPSFDMFVRTNELSNRREDRYSYYRSYMGSYNDNYPIANHYVLGY
                     HMTTYGRREYGSMLWNDTFKDMGGNIIRFDHFNYSLTRNTGDKTNINKLYQKTYDDLR
                     QKWQEQLEGLEFTESRQLTNVKSRRWTSLTQIGEYNGHLYAIQVGQDSLSGIVDISGK
                     SPVFLKQISSGPAFSGARNNERTVSFGGGKAVWTDLLPDARWDYRSFSSINVYDLKSK
                     KSQRLFPKGKFISATISRDGKKIAAVEFNSRRECSLVIIDSASKKIILRKDSPDNSFL
                     FDPSWFDNGENIAVCSLDDRGTSLLNFNANTGTFSTVIDYTHDENITTPYPSGNYIFY
                     QSDYSGINNIYAVDLNSTKRYQVVSARYGAFLPSVNQAGDTLYYSDYTVKGHQAKSLE
                     IDPTKWTPLENTVVKRIDYFKPVIDQEAGKSIAEDVPQKDYASKDYSPALNSINIYSW
                     SPIGDYGLGLVLYSVDVLETTSTELGYHYNYNEKTSSVRGNLEYRGYYPIFGIGGIYG
                     DRSKSLDDSWTKNIVWRESTGYGYVYLPLNLSRGIHTTYFELRTDAQYTEISDKSHTE
                     NNLRTIDDGQLTSVSYSALFSHSTQGMLLDIYPRWGQLLMANFRHTPQGDYFGEQISL
                     FSKLYFPSIFKHHSLYFEGAYQFDRYKYGEDNNYRYQLSFLYPRGYDELFFEKFYKGS
                     INYTLPLLNTNFNIWRMFYIKRIKLNGFYDHGYGTYQSNSFYSRSAGSELVLEYHPFS
                     NKHIVLDTGIRYAHLIESRENVYELVFRLSNE"
BASE COUNT         3940 a         2413 c         2736 g         4243 t
ORIGIN      
        1 ctatttgttg ccgagagtga taatcagatt attggctgga taagtttgag tccttatcga
       61 ggtggcaggg aagggctgtt gaaaactgta gaagtcagct attttgttga taatgatttt
      121 caacaacgtg atgttggaag taaaatgtta aaaaaaattc ttgagaaagc taagaatctt
      181 gaatatgaaa ctgttgtcgc aatcatcttt gagagtaatt taggtagtaa gaagttattg
      241 caaaaaaacg gtttcgaaag atggggttac ctgccggatg ttgctcaaat tgacggtaaa
      301 aggttctctc atggttacta cggtctaaaa ctgtaattca ttttactact tcgaatttgc
      361 cgatttatca aaatgaccat tttggataaa gtggataacc tgctctatta catcattatc
      421 actcataata aaggtatgtg tgttgtgaac aacgatgaaa tctttcatac cagaaagctt
      481 tgctctttca attgaaactt tcccgtcatc atctcctgga ataagcagtg aaagaatggg
      541 gtttatcgat ctatcaccgg taataattcc tacttcataa tatggagccc caacctggtt
      601 gggaatactt ttgctgcctg ttcccaactg ctgcccggca ggtccgttca gccatttgaa
      661 tacgaaaaag tctttcaatt tgtctacggt ttcgctgcct cgattgggcg ggcttaacat
      721 tacaacacga ccaagatttt caattctgtt tttatcaaga taataacgca caagaatgcc
      781 gcccatggag tgtgttacga aatgaatttt ttgcggctca aattttgcgc attcgctaat
      841 tgcaatgctt atcgtctctt ccgagagcgg ctgtatctct tttgatcgcg aagggtagcc
      901 cttgtttata acatggtaac cttcagttat cagtgattgt tccatttttg tcatagagct
      961 ttcggtacgg gcaagcccat gaagaagtat tacgcagtgg ttatttttct tattaccatg
     1021 attactattt ttttcgccgt gagtagccgt gatgaaaaaa aacagtattg ttgggataat
     1081 cagaacagtt aatcttttta tcatattatt tctttaattg tgatgctgat aattttttga
     1141 tcaattgtcc gggttacatt aaagcgggac aattatattt aatttgtaat tctaatatta
     1201 cagaggaaaa aattgcaata ataaaaaaaa agtcgtgagc atcatccata ccgatccctg
     1261 atctccgata tgtatcgttt gcctgtcgaa aaatggcttg gtgggattac attgagtaca
     1321 aagtcagaag ttagtatatt aactgtttgg tagtgtttca aatgaatttg atgtctaatt
     1381 ttataaaaat taaaacgagg ttttcaaata ttatacaact ttatgcgaat atccaggttg
     1441 ctgtatatga aaaatacaca gtccagtgat tgtatatcac gggactgtgg tgttgaaaga
     1501 cataacatgc tgtttgcatg cagcaggtct gatgatatca ataaccttaa ctggtattat
     1561 tacagctgtt cttttgcaag ttgggtttta tcaggagtct ttataacccg actactcttt
     1621 tgtgtaatcg tagtctgtac tcataacaaa gtcgtaatat aaagtaagtg tattgccaac
     1681 aagtacatat ggggttagac attcataata tgagcagtca ggatcgtcag acatctctgt
     1741 tattatctgt cgtatatatc ccgattctgt tccagacgcc tcccatgttc cagaaccggt
     1801 ctggctctca tgtgaatatg aaaaggttcc gtcactgtta aaagttgcac tgctacctgt
     1861 cattgcccaa gttccgacta aatttgaaga agcagaaaat tctgttttaa tcagggaaaa
     1921 cttcacgcca tctttgtcaa tcgtcattgt atcgtcatca atggtaaatt cagggttgag
     1981 tgtttccact tcatcaggta tttcgtacca gcctgtaccg gtaaaagtaa tgccttcctc
     2041 cggatctcta tcaagccaca tatgggtaga agtcagctga aaagtgttct catcaacagt
     2101 ataggtagat ttcattccgg aaatctgttc tgttgcgctg tcataaaaaa tcatagttga
     2161 gttttcagcc gtcacttcta ataaaatggc ctttgccatt actgtatcag gagaaggagc
     2221 ctctatctca tcgtcaagaa cagccatcca ttttcctttt ggatcaacat cattgctatc
     2281 tttgtcatca tcatctccat cacaagctgt aatgataaaa agcaagaaag aagtcaatat
     2341 gattgttaaa aaggtgagaa gtgattttga aaattttttc atgtgagtcc tccgtaaaaa
     2401 tattttggtt atactgcaac atggaagaca agcaaattta agaatgggat tgtaaagcgg
     2461 tgaaataaag atgggttaac agcaaaccta tttaggctta ttctcaaaca tttttgatga
     2521 tgtgtcaagg gttattaaag aaaaaatgaa ggctactaac agtaatcaga ttgtattgct
     2581 gttgcgaaaa cactgcctaa gtaaccgact atgttttcgc aacatgatgc ttgttttgtg
     2641 ctaacgcttg tcgtcaatgt aggttactac acctgcaagc tgtcgctccc agtagcctgc
     2701 aaattcggct tcagttacgg ttttaccggt gcttggcgag tgaataaatg tggtactgct
     2761 tattatcatg ccgacatgag aaacaggtga atagaaggcg actatatcgg ccaattttgc
     2821 tctatccata cccacgcttg ttccgttgct gtacatgtcc cggcttgttc gtggaagtgt
     2881 tgtaaaatca tattcgccat atacataata aatcaatccg gaacagtcaa agcccgatgg
     2941 tgttgctcca ccgtacagat aaggtgttcc tatctgctct cgtgcaagta gtataaggtc
     3001 gtcacgtaag agtgatgact caagatcggt atcaatgacg cttgaccact cttcactttc
     3061 ttcgctctca ccgggattac cagaattgtc atcggattcg ccgcttaaaa tatcgtcaac
     3121 aatttcgcat ccagatataa atatggcaaa aacggcagtt atacttatga ttattttttt
     3181 taacattggt attacctcac catatccgca atttgtgtgt ttccatcagc aacagcatag
     3241 tcatagggac gtttgccgct gatatctttt atctgtttat tagctccttt tgcgatcagg
     3301 tattgcgcaa tatcctccct gttttttgat actgcgtaaa atagggcggt ccatccttcg
     3361 ttatcttgtc tatttatatc cgctccgttt tcaattaaga ttttaactga ctcaaaatca
     3421 ccgtcataac aggcatacat aagagctgtt ttgttaaatc tgttcggaat gtcagggctt
     3481 ttgccggcct taagtatttt tgataacgtt gtatggtcgc cggaacgagt cgtatcaata
     3541 atagaatttt gagctgctat acctaaataa aagcagaaaa taattgttaa aagcgctatc
     3601 tttttcatgg ttgctccttt tgtctttaag tgagaaaacg aataatgata ttttaacata
     3661 atccgtgagt cttaatgttt cttgtctgtt attaccttta gtaattatcg gtagttatgg
     3721 ttacacccaa aggtaaaaaa atacccttaa ttaatgctaa atgtgtctat tcaaacaagc
     3781 aattgtctaa taaaattggt taatattttt taaaacaggc agatatatag ctaaagataa
     3841 tcaaagtatt gtttttatta cggttttgat ataaatgcat aaaattgtga taaaacgtta
     3901 ttagcattat gtgttgacaa caatcacaaa ataaatattg tattagataa atgttacatt
     3961 ttatttcgga gttaatatgt ccatttctca acgcaaacag cagtttataa aaaaaatagt
     4021 caaactctat tcgaatagtg ggctgcctat acattatacg agtctggcac aggagattgg
     4081 tgtaagcaag tggtcttctt atgagatgct catagaactt gaaaaacttg gatttatcga
     4141 acgacagtat cagaataacc gaaacaagag tggtcgttca agaaccatat tctctcctac
     4201 cgaaaaggct ttgaccctat ttgaaacgct ttctggcgag caagatatgc cagttgatga
     4261 gaaagtatgg aataacaaga cgtctctttt gaaaatagaa ctagatgaac ttaaagctct
     4321 tccggtgggt caatccataa acagtgtaat aggcaagata gagacagaag aggttaaact
     4381 ttttaaaatg gcctatatgt tttttatcta cctgataatc gctttcaaaa gcggtacctg
     4441 tattagtaaa gaaattatcg aaattattcg gtctgtcagt tcgcctgttt cacaatatat
     4501 gatgttatca ggagctacgc tggaaaatct tttggaaaat aatattgtag attctcccga
     4561 ggtaatgttg cctcttgtaa agcgctcgat aaacaatata tcttcgattc cctcagctga
     4621 tttaaaaaaa cttatcgaat tttttctgga tattgtttga aaatattatt tcaagaggta
     4681 tcttatttga gcctggcaag acattgcctg taaaatgtct cggcatctat ttctcttccg
     4741 cttttatata ttatcaaata gggcttgccc gaaaataggg agtgggaagc aagtttttcg
     4801 ataaagtcat ttacggtttt gatccgtgtc cctgcctttt ttctcttcat tttaagatgg
     4861 ttgcccgcgg ctatggaatc gtacgatttt ccgttacgga caaactgtgc gtccttgagg
     4921 tttattacgc attgaataag atagtcgatc ttctgttctt cactcatatc actttttgtg
     4981 gcttcttctg caagtgtgga tgttgttaga aacagcatca gtattaatat gtttttcact
     5041 attgcttctc cttttgactt tcaactgtga tattgacatc tcagcaaaaa aataagctgt
     5101 aaactggatt taactacttt atagtgataa cctctttatg atatttttta gaagttcctt
     5161 tatgtttgaa atattggaaa aaggatactg ttttcataaa aaaatgcaat aaaaattgtt
     5221 tattttcgtt ataaatagta aagggcaata aaattgtgtt acatattatt atctcactgt
     5281 ttgtgtggtt ttaatcgcaa tgattaagag gacgatttta acgggaaatt ctgaaatata
     5341 tgaatattgc aaaagataaa attggcgggt tttttaaaca agaatacaaa cggatgaaag
     5401 gttttgttca atcttatatc agtgattgtg tggatagtga gagcgatgat attatacagg
     5461 atgtaatgat caaactgagt tcacttgcgg atgtaacagt tccactggat aatctttcat
     5521 catatgttta ccgggcttta aaaaaccgaa tcatagatat atatcgttca gataataaaa
     5581 acaaatctgt accaattgag agtaataacg tagaagtggt ttcattatca gatgattcgc
     5641 ttgatgtctc ccaaattttt gaagaaagtc agttgagaga gaatctgatt gcggcattag
     5701 gccggctttc agagtccgaa cgcactctga ttatcatgac ggaatacgag ggaaaaacag
     5761 ttaggtatat aagtgaaaaa acaaatgagc ctgttggaac tctcttggcc agaaaatctc
     5821 gggcggttaa caaactccgc tattacatga aacagtacat ataaaaaaat actatatatg
     5881 gagaaatgca atgaatcatt tttggaaaac aaaagatgga aaaccaaagg tcttacatat
     5941 catcgctggt gtttccggcg gtataattgt tgcttgtatt tttgcatttg tgttttcatt
     6001 tgccgtacaa tatctttgga actggctatt aactgatctt ttcggattat ccagaatcga
     6061 cctttatcag tcgttcggat tagtaattct tgccaaaata ctctttggag gttttgggca
     6121 tggtcatcac cacaagccgt acggcgacca ggatagtcag caaaccgtag cggtaaccaa
     6181 ctgttgcggt gagtgcagtt gttatgatga tgttaaggaa aattgggtta gttacagcga
     6241 tttctggcaa gaagaaggac gtgttgcctg ggaaaacttc aaaagaaaaa aggatactac
     6301 gaacaagtaa aaaaagaggc cgaatctcaa tgttcggcct tatgaatgca agcaattgtg
     6361 atcctaagct tgttcagaga ttatttcatc aggaataatc tcttgtaaga gcgcgctact
     6421 gtcttcaata atgattctta gggttgtttc atcgctgtaa tcggcaaggt caattctgcc
     6481 cggtagaggt ttttgtttaa aacgaaaacg acctgggccg aataccaacg gaccgttctc
     6541 atccgcttta catagcgccc aaccctttag ggttctgctc aacataaggt cgctatgggt
     6601 cagctcgcct cgggcgggat tcagttccga taatattgtg tgcggattaa gacagagcac
     6661 tggcaattcc ttgatctgca tatagtgcaa agggtttacc agattcaata ctttgtacat
     6721 aaagcctccc gccttagatt tgttcatata aaatgttgct gattatcaga tacataagct
     6781 gtatgattta tgtatctgaa aaattatatt attgtctctc ggcaatgggt acatattgct
     6841 gtttgggttg tacacacttg tatgctggac gaataattcg tccacctgaa ataatctctt
     6901 caatgcggtg cgcacaccat cccgatattc ttgacatcgc aaaaagaggt gtatatatat
     6961 catctggtat atccagcatc tgatatacaa atcccgaata catgtcaacg tttgcagcaa
     7021 tgattttttc attctgtttg atctcatgaa ataatccagg tgtgagcttt tcaatgttat
     7081 aatagagatt aagctcttct tcatggcctt tttcgactgc aagaagttgc gctttctctt
     7141 ttaacagtat tgctcttgga tcgctatagg tataaattgc atgtcccata ccgtaaataa
     7201 gaccgtttcc gtcaaaagcc tcttttctga ttatttttgc cagatagtcc tcaagttctg
     7261 attcatttga ccagttttta acgttggcct tgatattgtc aaccatctga agcaccttaa
     7321 tgttggcacc accgtgtttg taccccttta atgaaccgat cccggcagca atagccgaat
     7381 aggtatcggt atcagatgaa gttatagcac gtactgtaaa agcggagtta ttacctccac
     7441 cgtgttcagc gtgaagaacg agggcaagat caagtatttc agcctcaagc ttggtgtagt
     7501 tgccatcaga acgtaacagt gacaaaaaga cttcagccgt gcttaaatct tcgggaatgt
     7561 gacgcaggtg aagagtccca ttgtcatagt agtggtgttt tgtgtgataa gcatacgcag
     7621 tcagcacagg aaatcgtgaa ataagcataa tgctctgttt gataatattt ttaagatttc
     7681 gatcttcagc ttcatcatcg taagaatata gagaaagtac tgttctcgca agtttgttca
     7741 tgatattagg gcttggagcc tttaatatca tgtcttccgt gaaattctcg ggtagcccac
     7801 ggtaactgtt aagaagatct ctgaactcca gatgctgatt ctcgtttgga agatctccaa
     7861 aaagcagaag gtaacaggtc tcttcaaatc cgaagcgatt gtcagtttga aatcctgcta
     7921 caatatcacg taaatcaata ccacggtaca tcaaacgtcc ttcaaccgga gttttttcac
     7981 tttcatccat tacatatccg tgaacctcac ctatttgagt cagtccggca agaactcccg
     8041 tgccgtcagg attgcgaagc ccccgcttag gccggtattt gaggttaagg tctggattga
     8101 tactgtttct gttcagagtt ctctgttcga ggtcgctgaa gaaatcgtct gtttttttac
     8161 tcatggcgtt tctcctggaa ttatatttta tataaatagt aaataatggc caaatattcc
     8221 cgttttcagg gcaccggctt ccgctggtaa ctgtacacgc agacaaaaaa aagaggaatg
     8281 acaggaatag ggaatccgga tttaaaaaaa cgaaaggttt gaacaataaa atcatagagg
     8341 ggtttttttg actactagtt tttgtaatta ttagctgtaa tgtctaaaaa atcgtataaa
     8401 tttgggaatt tgagcttttt tctttgattt atggcgtctt aagcagtttt atggcagaac
     8461 tattataaac attacgatat gtgaggatcc tatggtttac ggttctatat tggacacaat
     8521 cggaagctct ccttgtgtta agcttaacag acttaatccc tatcccgaag tacagatgta
     8581 tgcgaagctt gaaggattta acccgacagg aagtattaaa gacagaattg cactaaagat
     8641 ggttgagcag gccgaaaaag agggaaccct taatcgtaaa aaaattatta ttgaaccaac
     8701 cagcggtaat accggaatcg ggcttgccat gattggagcc gttaaaggat accgtgtgca
     8761 tatagtaatg agtgaagccg tatctataga gcggagacgc atgatagagg cattcggtgc
     8821 taaggtcata ttaacagatt cgtcaaaagg aactgacggc gctatttgcg aagctcaccg
     8881 cattattgag tctgatccag acagctattt tatgcctaat cagttttcaa accattataa
     8941 caaaatggcg cattacaaga caactgctgt agagatatgg aacgatactg agggccgggt
     9001 aacacatttt gtttcctccc ttggaacttc gggcaccctt atgggggttg gtaaggggct
     9061 gaaggagaaa aatccgaata taaaaatagt tgaagcgcat cccgaaaaag ggcattatat
     9121 acagggtttg aaaaacatgg aggaggccat agttccagag atctatgatc catctatggt
     9181 cgattgttcg gtaatggtta gaaccgatgt ggctttcgcc gcggcaagac agatagtttt
     9241 aaaagaggga atctttgcgg gcatgagttc cggcgctgct ctatatgcgg ccttagaagt
     9301 ggcacataaa atacgagagg gtgttgtcgt tatgatcttt cccgacaggg gtgagaaata
     9361 tttgagcaca gatctttttt taaatagcgg tgacaataat gagtaaagaa ggatattgtt
     9421 tggttctagg cggtggggga gcgaaaggag tttatcacgc tggagtctgg caggccttgc
     9481 gcgagctgcg tgtgcctata agtgctgtag tgggaaattc ggtcggcgct ctcgtggcag
     9541 gttttatagc gcaaaagaat attaagggac ttgattctgt tatggaaaac cttggtttaa
     9601 cagatttggt ctctatcccc gaaaagcttg taattgatgg ggaatttaaa cttaccagat
     9661 ctaacttcaa ggaatttatc agttttagaa aacaattatt tgaaaaaaag ggacttgata
     9721 cttcgccgct aaaaaaaatt cttaataatc atcttgatga atccaaactg cgttccttgg
     9781 gaattgacct tggtgttgtc acatattcac tttccagttt taagccgata gagatatttg
     9841 ttgaccagat gcctgaaggt acagttctgg attatctttt ggcaagtgcg actcttcccg
     9901 gattttctgc tacagaaatt aacgaaaaaa aatatatcga cggtggcgtt catgataaca
     9961 tccccttcgc gatggcaaag aatagaggtt acaagagaat tattgtttcg gatatttcag
    10021 gattgggtat caagcgaaga cctgaaataa cnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    10081 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    10141 nnnnnnnnnn ngaagatgcg atgcagcaaa gatttagtct atagtcttgc cgattgtgcc
    10201 gcttcggctc ttattattga aagggtcaag ttgtatcgct ttgaagatct gattaaggaa
    10261 attgaattaa aggccgcaaa cgttgatttt agaatcaaaa agttaaagaa agctataaca
    10321 ggacgagaac tacgatcatt gattcgtcaa attaaaaaaa tatcaaaaca gggtataggc
    10381 ttgagcgata tacaaaattg tccatggttt tattaccgta ttgtttcaga tattgtaccc
    10441 gagcctctta gaccagtgtt gttttcatca attacagcat tgtatccgga gactgcatca
    10501 tgtgctctgt ttttaaagat tattgggaaa atgtaaattt gagtaatgtc aactgataat
    10561 gattatgaaa aattaacagt atgtttaagg ttattcatta ctaagtctga aaactaactc
    10621 gtaaacattt tccctgctct caatcaggtg agcatatctg attcctgtat caagtacaat
    10681 gtgtttgttt gaaaacggat gatattccag tacaagctcg cttccggctg atctgctgta
    10741 aaaagaatta ctctgatatg taccgtaacc gtgatcgtaa aatccgttta acttgattct
    10801 ctttatataa aacattctcc atatgttaaa attcgtgtta agaaggggca gcgtgtagtt
    10861 aatacttcct ttgtaaaatt tttcaaaaaa cagttcatca tatcctctgg gatacaaaaa
    10921 gcttaactga tatctatagt tgttatcttc accatactta tatcggtcaa attgataagc
    10981 accttcaaaa tagaggctgt ggtgtttaaa tatggatggg aaatataact tcgaaaagag
    11041 agatatctgt tcgccaaaat agtctccctg tggagtatgg cgaaagttgg ccataagaag
    11101 ttgcccccat cgaggataaa tatcgagcag cattccttgt gttgagtgtg aaaagagtgc
    11161 actgtagctt acagatgtca actgtccgtc atcaattgta cgcaggttgt tttccgtatg
    11221 cgatttatca gatatctctg tgtattgggc atcggttctc agttcaaaat atgttgtatg
    11281 aatccccctt gaaagattta gaggcagata tacatacccg taacctgtag attcgcgcca
    11341 gacgatattt ttagtccagc tgtcgtcgag agattttgaa cggtctccat atattccgcc
    11401 aattccaaaa atcgggtaat aaccgcgata ctccaagttt cccctaacag aactggtttt
    11461 ttcattgtag ttgtaatggt aacctaactc ggtggaggtg gtttcaagta catcaacaga
    11521 atatagaaca agccccagtc catagtctcc aatcgggctc caggaataga tattaatcga
    11581 atttaaagca ggagaataat cttttgaagc ataatctttt tgcggtacat cttctgctat
    11641 gcttttgccg gcttcctgat cgatcaccgg cttaaagtag tctattctct ttacaacggt
    11701 attttctaaa ggagtccatt tcgtcggatc aatttcaagg cttttagcct gatgtccttt
    11761 tactgtgtag tcggaatagt agagagtgtc tcccgcctga ttaaccgatg gcaagaatgc
    11821 accgtatctc gcagaaacca cctgatagcg ttttgtgcta tttaggtcaa ccgcgtagat
    11881 attgttgatt ccggaatagt ctgattgata aaaaatataa tttccggacg gataaggggt
    11941 agtaatattc tcatcatgtg tgtagtcaat tacagtacta aacgtgccgg tattggcatt
    12001 aaaatttaaa agagaggttc ccctatcgtc aagggagcag acggcaatgt tttccccatt
    12061 atcgaaccac gagggatcaa aaagaaacga attatctggt gagtcttttc tcaagattat
    12121 ttttttagat gctgaatcaa ttatgacaag cgaacattct cttcgtgagt taaactcaac
    12181 agctgcaatt ttttttccgt ctctggatat tgttgccgaa atgaattttc cttttggaaa
    12241 tagcctttgt gattttttac tttttaaatc atatacattg atcgaggaaa aacttctgta
    12301 atcccatctc gcatctggta gaagatctgt ccatacggcc tttccccctc cgaaggagac
    12361 agttctttca ttgtttctgg cgcctgaaaa agcgggaccc gaggatatct gtttaaggaa
    12421 caccggactt ttgccgctga tatcgacaat accggataaa gagtcttgac caacttgtat
    12481 tgcataaagg tgtccattat attcgcctat ctgggttaaa ctcgtccagc gtctagattt
    12541 tacgtttgtt agttgtctgg attctgtaaa ttcgaggcct tcaagctgtt cttgccattt
    12601 ttgccttaag tcgtcatagg ttttttgata cagtttgtta atatttgttt tatcacctgt
    12661 gttacgggtt agtgagtagt tgaaatggtc aaatctgata atattgccac ccatgtcttt
    12721 gaatgtatcg ttccacagca ttgagccata ttcgcgtcga ccatatgttg tcatgtgata
    12781 acccaataca taatggtttg ctatggggta attgtcattg taagaaccca tataagaacg
    12841 gtagtagctg tagcgatctt cacgtcggtt cgacagctcg tttgttctga caaacatgtc
    12901 aaaagagggc tgtcttcctc ggccaccgtt tgtgagagtt gtctctgcaa gaacagcatc
    12961 tccttccata atccatgaag gaacaaaggc taccgcgtaa atagcggctc cgatatcacc
    13021 aaaaacgtac caagtaattt tacgcccgcc actgttaatg gtgttaagct ggctcatatg
    13081 tcgtatttcg tgaaccgaaa gagcagtaaa ccattcgttt gcagcgaatt gaccaggtgt
    13141 gttgtaaaaa tgcgatttgt atggagcata aaacaccgag ccatttgctt cggcgctttc
    13201 agaaataagg actatcgttg tgcgcctcca gtcgcttttc atggttttgt tatggtgttt
    13261 cagatagtgt tcagcaagat tcgcaactct ttgagcgtcc tcttctatgt ccgggttgta
    13321 aattatctga aaattttcag tatctattga tttccactta agagaatagg ggtgtcccgc
    13381 ttcaagattg tgaaaacata aaaggaacgt acaaaaaatt gtaaatatga tc
//
LOCUS       DAOTGZ010000058        13329 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_3064
            NODE_5555_length_13329_cov_5.028477, whole genome shotgun sequence.
ACCESSION   DAOTGZ010000058 DAOTGZ010000000
VERSION     DAOTGZ010000058.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868088
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 13329)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 13329)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:22:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,785
            CDSs (total)                      :: 2,751
            Genes (coding)                    :: 2,732
            CDSs (with protein)               :: 2,732
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 12 of 19
            Pseudo Genes (internal stop)      :: 5 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13329
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5555_length_13329_cov_5.028477"
                     /isolate="AMR_MDS_3064"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(161..868)
                     /locus_tag="P5123_05550"
     CDS             complement(161..868)
                     /locus_tag="P5123_05550"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: protein motif:HMM:NF012521.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HRX15756.1"
                     /translation="MRLYIVRHGDPDYQNDILTSQGELEAQALAERIADEKPDLLYAS
                     PLGRAKRTAWHSENKTGLKAEILDWAREWNFLTIPESFMGRVAAWDIPGEIYHKIIDG
                     NYDSSLYSGLTPDESKISDAFESLAESSDRFLATLGYEKDANSIYRIRERSERKIFLF
                     CHNGFGLAYLSYLLSIPYQLVWSSFWLPTTSVTTILFEERLEGIAVPRALNVGDTSHL
                     YKKGLPVLPQGVKANYY"
     gene            complement(886..2820)
                     /locus_tag="P5123_05555"
     CDS             complement(886..2820)
                     /locus_tag="P5123_05555"
                     /inference="COORDINATES: protein motif:HMM:NF012707.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter substrate-binding domain-containing
                     protein"
                     /protein_id="HRX15757.1"
                     /translation="MRYLYLLLLWILFFLNCQAFANKLDLTEEEKLFIKEHSVITVVS
                     DANWPPVEYADDFGDNIGISADYLRLVEERTGIKFKRIKGISWQNAYKKLQSHAIDMT
                     TCVRPTPLRESFLIFTEPYIEIPTVIVTHFSVAYVSHLGELNGKKIAVVRNYAPAELL
                     KQDFPDINLVYVNSVEEGLNLLDNNQVFALIDNMLVVGYYIAINNMYKLKIAGETPYK
                     YSMSMAVRKDWPQLLTIIQKALDSISPEERKKIYNRWVAVRYEYSFNYVNLIRAALGM
                     SVLILLMLFWIRRLRREIKQRIMAENSLAGMEQRLRIHLLETPLGIIEWDMNFSIIEW
                     NPACERIFGYSKGDVLGKNGDFMVQPADREKLSSIWNKLSRSRDSIYNLNENIKKNGS
                     IIVCDWINTPIIDSSGDTVSIISLCRDITDDVAGRNRIRQSLKEKEILLRELYHRTKN
                     NMQVIIAYLLLQSDKINEPVVHGLVKKTVSYIQSMALVHQKLCQSSSLVLINISEYLN
                     ELIKLIQGFYSGETSKITTDVSCQSFNINMDTALALGLVVNELVINSYKHAFGLGEEG
                     SIVISLKKTENNVVVLNISDNGRGLSKEIDLTQMGSVGLLTARSIVESQLNGTLSVRN
                     EGGLSIEIVFSIKNEPPDNY"
     gene            3114..3434
                     /locus_tag="P5123_05560"
     CDS             3114..3434
                     /locus_tag="P5123_05560"
                     /inference="COORDINATES: protein motif:HMM:NF018894.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0035438 - cyclic-di-GMP binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PilZ domain-containing protein"
                     /protein_id="HRX15758.1"
                     /translation="MEEKRNYERVGKKLRAEVHVDDGMTFSASVDISMGGVFISTPEP
                     LQEGQHVDLAIYASNDDPVNVSGNIKWTRDETDDNVAGMGIQFTDMTEGDKTKIKEII
                     ANYN"
     gene            complement(3499..6345)
                     /locus_tag="P5123_05565"
     CDS             complement(3499..6345)
                     /locus_tag="P5123_05565"
                     /inference="COORDINATES: protein motif:HMM:NF012403.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0140658 - ATP-dependent chromatin
                     remodeler activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SNF2-related protein"
                     /protein_id="HRX15759.1"
                     /translation="MDNRLIEDQAADLFEVYSENVKTVEKHDPYTSIEGVDRVLGLLK
                     YGQLRPDPRFQLKNEAYLLKLAYANNKLLSLSNSRTKILAHQVESTYIVVNTLNQRYI
                     LADEVGLGKTVEAGLIIKEFVYRYNYRRIMVAAPASLLVQWQLEMEEKFGESFEVLDR
                     KVLEKRRRELGPGENPWNSFEKIICSLDFIKNPAFRDELAATSWDAIIFDEAHRLRRD
                     ANSSTIIYTAAEMLSERTKALLLLTATPFRGKLEELFYIVKLVDKNLLGPYQSFYNEY
                     CSADADLSTLKEKLSTVLLRRTKNEIGGFTKRHARTIRFDLYPEERELYDETSRYVAE
                     EFNRAMQTQNRAVGFIMTVFQKLLDSSSHALHCALCNRKRSLEAKLDRADRYLKACVE
                     YDSIEFDDLCDDLEDEGSLCDATVQKTAEEIRIEIQILERLIELSGAITRNKKGDRLK
                     KMISGLKKEGRKKFLIFTQFKTTQDYLKKILTGLNVEVFNGSMDKDAKERAIDNFKKD
                     VEVLICTEAGGEGRNMQFCNVLFNYDLPWSPLKIEQRIGRLHRFGQKDDVFIYNFATK
                     DTVAERVLDVLEHKLHLFEESIGPPDIMLGQIEDELNLGSLFMKIRSGLTSKKESMEM
                     LDSSVLRAKKSFEKLSSLTLTRRMDFNYDEYYRITQTERRFSNEQLEHFMVRYASEFP
                     DCPFNPLKQDNFYNIRVSDEAGFSVMRGTFDSDLALSNSGSEFLAFGHSVVDSAIDCA
                     LSSDFGGFTSLKVISADKNFEGMVFTFEVRFKSVSAMSEYVPVFVSFNNRLDSFDIEE
                     TEDQFLLRPDIVTEISEDKLEESVNRLRPFSEELFSMAVECLKHKIADKVFDIKENLD
                     LQIDPEIEKVNESYNKQINELREVLDRQQLAEKTSGGMKGAITRTRNKIEQAEREKRH
                     LLARYKRYSGVSYTISIIGAMILVGMDKSPDNPR"
     gene            6605..7411
                     /locus_tag="P5123_05570"
     CDS             6605..7411
                     /locus_tag="P5123_05570"
                     /inference="COORDINATES: protein motif:HMM:NF012874.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRX15760.1"
                     /translation="MLIKSVMTILFFLQITLFAESATYLFLPNSSNFSIAISNNSYQI
                     IGTASSAERSFNNAIKQATTGERISIIGLDPAGKAVAEKIDTLIQEISQRSVNARDWN
                     SYLHYLDGIVSQISDTVRRIHLLGVKASNGIMGELEREIIQSEIDILISSITSTTNYA
                     TFNKKKIELPVNPEALGLTGIDVVNNPAATVGIADEALAKLNIIRSRKGAQSRMLEMR
                     IKGNSLRFIHMTSSYSVISDTDMVEVSTTLNLSKVRLQNAHGVLYRITRL"
     gene            7798..8967
                     /locus_tag="P5123_05575"
     CDS             7798..8967
                     /locus_tag="P5123_05575"
                     /inference="COORDINATES: protein motif:HMM:NF024242.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HRX15761.1"
                     /translation="MPETPKRISVIRRYRLLLFIVPVAIIHIILGQHFLNYRKPFHLF
                     PSANRSVISTTQNTEAGMASILWFQNNSYKAEISYVLSKDPLHNQEGTRPRAGLIFSN
                     PDRSFIDLSNYEKINLNLFTKESKSVEIVLRIFLPNFTTLGNEMTYLHLKYYLPVTTN
                     LTRFSIPLSRFDIPEWWLTINKLSRYDSVINVDLSKVQDIVLTNDEKHPFDINSKISV
                     TSIWFSPKDWDYNKLLFFLLPIYLIITVFFVAKYKRVLSKPGLNNIFFKKIQIQSRNQ
                     PDPDFLIDYIKNNSSESNLSLGTISSATLFPEKKISVIVKEKTGMNFSDYLKHLRIEK
                     AKELLLNSDDKIIDIAFNSGYNTINNFNFYFKEATGTTPKQFRKVYKNKNGNKTY"
     gene            8948..10024
                     /locus_tag="P5123_05580"
     CDS             8948..10024
                     /locus_tag="P5123_05580"
                     /inference="COORDINATES: protein motif:HMM:NF024242.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HRX15762.1"
                     /translation="MAIKHIKKILIPIVITTTAITAVISQKNLQVFPAVNTVISFYCD
                     QLNKGNTEIHYAESNDKHALITYTPREGFVTPYAGIGFTTRHRNALWDASEYDYINIQ
                     VETTRSRALRLFIYTHIDGISKKGNVHSYLFNLQEIPVWKQEKNYQIKLTEFNVPEWW
                     YNMNNIVPNDEKVYRDYSKTRSIEIQNGTIAQLDSKDTIVVKRIFFSRSKKNILIYWF
                     AANFIYLLIFLSIQYFRKKMDRPAAAKRNVSEKEREELEKITDYIGNHYMDSSLTVNM
                     VSQATEVSAYKVSILLKEEFTLSFSQYLNTIRLTEAKRLLQTTELKISEIASMTGFGS
                     INHFNRIFKLTENRTPAEYRQFNS"
     gene            complement(10049..10498)
                     /locus_tag="P5123_05585"
     CDS             complement(10049..10498)
                     /locus_tag="P5123_05585"
                     /inference="COORDINATES: protein motif:HMM:NF025054.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HRX15763.1"
                     /translation="METIFRLIDKQQFSLIRELWEKLNKIHYNDSVNFKNYFESFTFD
                     QRIKAFESIEDKYIRIEIVQIKDEIIGYCISTITNEIGDIQSLFIDSEYRSHGFGEKL
                     VNNALSWFRSADCNRYQVTVAEGHESVFGFYKKMGFYPRKTVLEYKN"
     gene            complement(10587..11186)
                     /locus_tag="P5123_05590"
     CDS             complement(10587..11186)
                     /locus_tag="P5123_05590"
                     /inference="COORDINATES: protein motif:HMM:NF012697.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRX15764.1"
                     /translation="MLVLVNGGSCSGKSTFSERLKQMLVESGVSCDCLSTDLFYKEIP
                     QNCNLYDYNFDTPAAFDNELFFETVNLIQRGKAFQLECFDFKTHSRSKIMSIKSCRVL
                     IVEGIFSFVDKKLVNSSTLNIFIDTDLQTRYKRRYKIYSQIMKHDDSFINHKFYNQAE
                     PYFESDIYPLRRYSHLVINGDKSFDVPLKCVTAYLRSIL"
     gene            11338..11859
                     /locus_tag="P5123_05595"
     CDS             11338..11859
                     /locus_tag="P5123_05595"
                     /inference="COORDINATES: protein motif:HMM:NF024231.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HRX15765.1"
                     /translation="MSTSENTLSLSSVPNILTETALIAIAIALPVVMHLIPGINVFAT
                     LPMHWTIFIAGLLYGWKGGLIAGVSVPTLSFALTGMPVPFVLPLMTVELSLYGFVTGL
                     LSEKTMLNRHLIVLISSIAGRAGFVILALLLSRTDSATQFAINSFAPGIIAAFIIIAC
                     TPVISKIAAKNLR"
     gene            complement(11893..12747)
                     /locus_tag="P5123_05600"
     CDS             complement(11893..12747)
                     /locus_tag="P5123_05600"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRX15766.1"
                     /translation="MIIKNKRLTVTLQRPGSVYRRSRFDWGAIVSSVILDDNVNFCSK
                     EATGDNLGTEGTGLTFEFGINKAVGYDEAKPGEFFPKIGVGFLKRESQDDYDFFHDYE
                     IKPVDISVKQDENVHFSQQSPILNGYGWILDRSISINENHLVIDTSLKNSGEKRIITN
                     EYCHNFLKLGENPVDASYLLSMNREVRLSESKGVVQHGKNSISPDGAPDSWLYCATEK
                     IDDSGEFHWKLTHQESGISVGCEEKFNVSRFALWGMKHVISPEFFIEIDLKPQESLAW
                     SRCYSFEK"
     gene            12889..>13329
                     /locus_tag="P5123_05605"
     CDS             12889..>13329
                     /locus_tag="P5123_05605"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRX15767.1"
                     /translation="MNIVKKTLLIMLFVLTSFTIVSEDYQYDYFSGDDPDRQKYVPFS
                     DYIPKVRKHYQTVPHYLEDYYLLYGMKQHYNENSLRKNIAMLQTAMECKFRHPIYALC
                     KIETEDEYYKYRNLMFMHLNLLIMRNHMKIAVRYDKQTIRFYDAD"
BASE COUNT         4294 a         2730 c         2230 g         4075 t
ORIGIN      
        1 atagacactt aaaccatgtt gccctctgga acatggtaat ttttcgaagt tccctgatgt
       61 ttatctaaaa tatagatttg gtaattttat cattaatcaa ctctatttca gccaattttt
      121 actgtcaagt cacagaaata tgaccaaaat tagttaactg ttaatagtag ttagctttaa
      181 caccctgcgg aagaacaggc aaaccttttt tataaagatg cgacgtgtca cccacgttta
      241 gcgccctggg aaccgcgata ccctcgagac gctcctcgaa cagtatggtt gttacagatg
      301 ttgtaggcaa ccaaaaactg ctccaaacga gctgataagg aatcgataaa agataagata
      361 aataggccaa accgaaaccg ttatgacaaa aaagaaaaat ttttctttct gacctttctc
      421 taatacggta aattgaattc gcatcttttt cataacccag tgttgcaaga aatctgtcag
      481 aactttcggc caatgattca aaagcatcac tgatttttga ctcatccggg gttaatccgg
      541 aatatagaga gctgtcataa ttaccgtcaa taattttatg atatatctcg ccaggaatat
      601 cccaagcagc tacccggccc ataaagcttt caggaatcgt cagaaaattc cactctcgag
      661 cccaatccaa tatttcggct ttaagaccag tcttattttc cgaatgccaa gctgttcttt
      721 tagcacgacc aagaggagaa gcataaagaa gatcaggttt ttcgtcagca atacgctcgg
      781 caagagcctg tgcctcaagc tcaccttgag aagttaatat atcattctga tagtcgggat
      841 ccccatgacg gacaatataa agacgcatcg atacctctcg atattttaat aattatcagg
      901 cggttcgttt tttattgaaa agacgatttc tatacttaac ccgccttcat ttcgaaccga
      961 aagtgtaccg ttaagctgtg actcaacaat acttcttgca gttagaagac caacagaacc
     1021 catttgagtc aaatcaattt ctttagaaag cccacgtcca ttatctgaga tatttagcac
     1081 tacaacatta ttttcagttt tttttaatga gatcacaatc gacccctctt cccccaaacc
     1141 aaacgcatgt ttatatgaat taataaccag ttcgtttaca accaaaccta atgcaagcgc
     1201 ggtatccatg tttatattga aactttgaca acttacatcg gttgtaattt tcgaggtttc
     1261 acccgaataa aacccctgaa ttagcttaat cagttcgttc agatattcac tgatgtttat
     1321 taaaaccaga ctgcttgatt gacatagttt ttgatgaaca agtgccatgg actgtatata
     1381 cgagacagtt ttctttacta acccgtggac aactggctca ttaatcttgt cggactgcaa
     1441 tagaagatat gcaattatta cttgcatatt gtttttcgtt cgatgataca gttctcgtaa
     1501 aagaatctcc ttctctttta acgactgtct aatgcgatta cgccccgcta catcatccgt
     1561 tatatcccgg cataaagata ttatcgatac cgtgtcaccg cttgaatcaa taatgggtgt
     1621 gtttatccaa tcacatacta ttatactccc gtttttttta atgttttcat taagattata
     1681 tatagaatca cgacttcgtg ataacttatt ccaaattgaa gatagttttt cccgatctgc
     1741 cggctgaacc ataaaatcgc cgtttttacc caaaacatcg cccttactgt atccaaaaat
     1801 tctctcgcaa gcaggattcc attcaattat tgaaaaattc atatcccact cgattattcc
     1861 cagtggtgtt tcaagcagat gaattcttaa tcgttgctcc ataccggcaa gactgttttc
     1921 agccattata cgctgtttta tttcccgcct gagtcttctt atccaaaaaa gcattagcaa
     1981 aataagaaca gacattccca gagccgctct tatcagattt acataattga agctgtattc
     2041 gtaacgtaca gcgacccatc tgttgtaaat tttcttacgc tcttcaggtg aaattgaatc
     2101 aagagccttc tgaattatgg taagcaactg aggccaatct ttgcgaaccg ccatggacat
     2161 tgaatattta tagggtgttt cgccggctat tttgagcttg tacatattat ttatggcaat
     2221 atagtaacca acaaccagca tattatcaat taatgcaaat acctggttat tatccagaag
     2281 attcaagccc tcctcgacac tgtttacata aaccaggtta atatccggaa aatcctgttt
     2341 caaaagttct gcaggagcat aattccttac cacagctatt tttttaccgt ttagttcccc
     2401 gaggtgcgaa acataggcta cactaaaatg tgttactata accgtcggta tctcgatata
     2461 gggctctgtg aaaatcagaa aactctctct caaaggagta ggccttacac aagtagtcat
     2521 atcaatagca tggctctgaa gttttttata agcattctgc caactgattc ccttaatcct
     2581 tttgaattta atacctgtcc gctcttcaac aagcctaaga taatcggccg aaattcctat
     2641 gttatctcca aaatcatctg cgtactcgac tggtggccag ttagcatccg aaactacagt
     2701 tattacagaa tgttctttta taaacagctt ctcttcttct gtcaaatcga gcttatttgc
     2761 aaaagcttga cagtttaaaa aaaacaagat ccaaagtagt aaaagataaa gatatcgcat
     2821 tattaaaccc gacaattata tttataaaaa aatttttcgc aagttctctt gctgatcgtc
     2881 cagcaccact ttgaaaacgt aaaaagatgc taggttgttc gcagatgcaa aacagtatca
     2941 acttaaccaa gttgccggca ttaatcaact gcacatcctg atatcctaat gttttttatt
     3001 tgactattag ccattatctg cttaatattt taattcaggt taatttaggg ttatcatgta
     3061 accgttaatc agcatagaga ccgctttttt gcagaaaaca aggagttact tttatggaag
     3121 aaaaaaggaa ttatgaacgt gtagggaaaa aacttcgtgc ggaggttcat gttgatgacg
     3181 gtatgacttt cagcgcatcg gtggacataa gcatgggagg agtctttata tcaacccctg
     3241 agcctcttca agagggacaa catgtagatc ttgccattta cgcgtcaaac gacgaccctg
     3301 taaatgtgag tggcaatatc aaatggacca gagatgaaac cgatgacaac gtggctggca
     3361 tgggtattca gtttacagat atgacagaag gtgataaaac aaaaatcaag gaaataatag
     3421 caaactataa ctaagtcaga ctgtctggca tcgcaaatct ttacggtgcc agacacacaa
     3481 tagatgtaaa attcaccgtc atcggggatt atcgggactc ttgtccatac ctacaagaat
     3541 catcgctccg atgattgaaa tagtgtaact tacaccggaa tagcgtttgt aacgagctaa
     3601 aagatgcctc ttctctcttt ccgcctgttc aattttattt cgagtacggg ttatggctcc
     3661 tttcatgccc ccagatgtct tttctgcgag ctgctgccgg tcaagaactt cacgaagctc
     3721 attaatctgt ttattatatg attcattcac cttttcaatt tctggatcga tctgaagatc
     3781 aaggttttct ttgatgtcaa aaaccttgtc ggcgatcttg tgttttaaac actcgacggc
     3841 cattgaaaac agctcctcac taaaaggtct tagacgattg acgctctctt caagcttatc
     3901 ttccgaaatt tcagttacaa tatccggcct tagaagaaac tgatcctctg tctcttcaat
     3961 gtcaaaagag tcaagcctgt tgttaaaaga aacaaaaact ggaacatatt ctgacatagc
     4021 tgaaaccgat ttaaaacgca cttcgaaagt aaataccata ccttcgaaat ttttatcagc
     4081 agatatcacc ttaagtgacg tgaagcctcc aaaatctgac gaaagagcgc aatctattgc
     4141 cgaatcaaca acagaatgcc cgaatgccag aaattcggag ccggaattgc tcaaagccag
     4201 atcggaatcg aacgtaccgc gcataacaga gaatccggcc tcatctgata cacgtatatt
     4261 gtaaaaatta tcctgcttca acggattgaa aggacaatct ggaaattccg aagcgtatcg
     4321 aaccataaaa tgctcaagtt gctcgttgga aaaacgtctt tcggtctgtg ttattctata
     4381 atactcatca tagttaaaat ccattcttcg cgtaagagtc aaggaagata gtttctcaaa
     4441 actcttcttt gcccttaaaa ccgaagagtc tagcatttcc attgactctt ttttactggt
     4501 tagaccgctt cttattttca taaacaaaga tccgagatta agctcatctt cgatctgacc
     4561 aagcatgata tcgggtggcc ctattgattc ctcaaaaaga tgaagtttgt gctcaaggac
     4621 atctaggact ctttcggcaa cggtatcttt tgtagcaaaa ttatagataa atacatcatc
     4681 cttttggcca aaacgatgta aacgaccgat acgctgttca atcttgaggg gtgaccaggg
     4741 taaatcataa ttaaacagca cattgcaaaa ctgcatattt cgcccttcac cgccagcttc
     4801 ggtgcaaatc aaaacttcta catctttttt gaaattgtca atggctcgct ctttcgcatc
     4861 tttatccatt gatccgttaa aaacttccac atttaaacca gttaatattt tttttagata
     4921 atcctgagtg gttttaaact gcgtaaatat aagaaatttt ttacgcccct cttttttaag
     4981 accgctaatc atttttttta gacgatcacc ttttttgttt cttgtaatcg cccctgaaag
     5041 ttctataagc ctctcaagaa tttgtatttc gatacgaatt tcttctgctg tcttttgaac
     5101 tgttgcgtca cacagactgc cttcatcttc aagatcatcg caaagatcgt caaattctat
     5161 tgaatcatat tcgacacaag ctttcagata acgatcagca cggtccagtt ttgcctctag
     5221 cgaacgtttc cgattgcata aagcgcaatg aagtgcatgt gaagatgaat caagtagttt
     5281 ctgaaacaca gtcattataa acccaacagc cctgttttga gtctgcattg cccggttaaa
     5341 ctcctccgct acatagcggc ttgtctcatc gtatagttca cgctcttctg gataaaggtc
     5401 aaacctgata gttcgggcat ggcgttttgt aaaaccgcca atctcgtttt ttgtgcgccg
     5461 caaaagtact gttgaaagct tttccttaag agttgagaga tcggcatccg cagaacagta
     5521 ttcattatag aaactctgat acgggccaag cagattttta tcaacgagtt ttactatata
     5581 aaagagctct tccaacttgc cccgaaaggg tgtcgcagtc aaaagaagca acgccttggt
     5641 tcgttcggag agcatctcgg cggcagtata tatgatcgta ctgctattcg cgtcacgacg
     5701 caacctatga gcctcatcaa atattatggc gtcccaactg gtggcagcca attcatcccg
     5761 aaacgcggga tttttaataa aatcaagaga acaaataatc ttctcgaaac tgttccaggg
     5821 attttcacca ggacctaatt cacgccttcg cttttccagc acctttcggt caagaacctc
     5881 aaaactctct ccgaatttct cttccatttc aagctgccac tgaacaagta aagatgcggg
     5941 tgccgcgacc attatacgcc tatagttata gcgatagaca aattccttaa tgattaagcc
     6001 tgcttctact gttttgccca gaccgacttc atcggcaaga atatagcgct gattaagtgt
     6061 atttaccaca atgtaagtag actcgacctg atgagccaaa atcttggtac gagagttaga
     6121 aagtgaaagc aatttattgt tggcataggc caatttaaga agataagcct cattttttag
     6181 ctgaaatctg ggatcaggtc gcaactgccc atacttcaga agacctaaaa ctctatccac
     6241 accctctata ctggtatacg gatcatgttt ttcgactgtt tttacatttt cggaatatac
     6301 ttcaaagaga tcagcagcct gatcttcaat taatctgttg tccactcgta accataactc
     6361 cgggatattt gtatattact cttaattgtt ctatttgaat tgaaagaaga cagctattat
     6421 cagataattc tggattaaag gctatacatg ccggtcttat gtcaaatagt gtagttattt
     6481 ccctgatgca actgtttttc ctgtaattgt tgtcaaagag agttaaattg gataacttag
     6541 ttaaagaccg aacaaagtga ttttctagta gaactcaata agtacgcaaa acagggataa
     6601 aaaaatgtta ataaaaagtg tcatgacgat actatttttt ttacagataa cactgttcgc
     6661 agaatccgcg acctatcttt ttttgccaaa tagttccaac ttcagtatcg ccataagtaa
     6721 taacagctac cagatcattg gcaccgcatc atcggcggaa agaagcttta ataatgcgat
     6781 caagcaggct actactggtg aaaggatatc aataattggc cttgatcctg ccggtaaagc
     6841 tgtagctgaa aagatagata cccttataca ggagatctct caaaggtctg taaatgccag
     6901 ggattggaat tcttatcttc actatttaga tggaatagtt tcacaaatct ccgatacagt
     6961 aagaagaata cacctgcttg gtgtaaaggc ctctaatgga ataatgggag aactggaaag
     7021 agaaataatc cagtcagaaa ttgatatctt gatctcttct attactagca cgaccaatta
     7081 cgcgacattt aataaaaaaa agatagaact gcccgttaat ccggaagctc tgggacttac
     7141 tggtattgat gttgttaaca atccggcagc aaccgtcggt attgccgatg aagcgcttgc
     7201 aaagttaaat ataattcgat cgcgtaaagg agcacaatca cgtatgcttg agatgcggat
     7261 taaagggaat agccttagat ttattcacat gacgtcgtcc tattctgtta tcagcgatac
     7321 agatatggtc gaagtttcaa caacgctaaa tctcagcaag gtaagattac aaaacgcgca
     7381 cggtgtactt tacaggatta ccagactata aatggatttt tttaagaaat atagcttttt
     7441 tttgaaaaat tagaaaatta tcaattaatc aggattattc tgttagattt ttattatcag
     7501 atatttagta taaatatcgt ttgatagtat gttatttaga aataaaccct catgggcatc
     7561 gttatcgatg cccttttttt ataagtcatt tcaaatacaa aaacccgccc atgcttatta
     7621 aaaacctttc tgaaaaaatc tttgacctaa acaatattgc tttttgcctt ttgttgcttt
     7681 caaacacaat ctcgctttaa tacacaataa tttaattgac gtaaacttca aaaaaaaatc
     7741 aaatcggcag aaggattatt caacaacgtt tctatataac caaacaggag ctttcggatg
     7801 ccagaaacac caaaaagaat atccgttata cgcagataca gattattact gtttattgtt
     7861 cctgtcgcca ttattcatat tattttaggc caacacttct taaattacag aaaacccttt
     7921 cacctatttc cttccgcaaa cagatcagtt atatcaacaa ctcaaaacac agaggcgggg
     7981 atggcttcaa tactctggtt tcaaaataac tcatacaagg cagaaataag ctatgtgctc
     8041 agtaaagacc cactccataa ccaggaaggt acaagacccc gtgccggtct tattttcagt
     8101 aaccctgacc gttcgtttat tgacctatca aattatgaaa agattaatct taatcttttt
     8161 accaaagaat cgaaatctgt agaaatcgtt ttgcgaatat ttttacctaa ctttacaact
     8221 cttggaaacg aaatgactta tcttcatctc aagtattatc tgcctgttac aacgaatttg
     8281 actcgttttt caataccttt aagtagattt gatatcccgg aatggtggct aactatcaat
     8341 aaactatcca gatatgattc tgtaattaat gttgaccttt ctaaagtcca ggatattgtc
     8401 cttacaaacg atgaaaagca tccatttgat attaattcaa aaatctctgt aacgtctatc
     8461 tggttttcac caaaagactg ggattataat aaacttttgt tttttttatt accgatttat
     8521 cttattatca ccgttttctt tgtagccaaa tacaagcgag tactatcaaa acctggacta
     8581 aacaatatat ttttcaagaa aattcaaatt caaagccgaa atcaaccgga tcctgatttt
     8641 ttgatagatt atattaaaaa taattcttcc gaatccaatc tctcactcgg aaccatcagt
     8701 agcgcaactc ttttcccgga gaaaaagata tctgttatag taaaagaaaa aaccggtatg
     8761 aatttttcag actatttgaa acatttacgt attgaaaaag ctaaagagct tttgttaaat
     8821 tctgacgata agattataga tatagctttt aactctggat acaacacaat aaataatttt
     8881 aatttttatt tcaaggaagc gaccggaaca actccaaaac agttcagaaa ggtatacaag
     8941 aacaaaaatg gcaataaaac atattaaaaa aattttaatt cctatagtta tcacaactac
     9001 ggccataaca gcggttatta gtcaaaaaaa cctacaggtt ttcccagctg tgaatacagt
     9061 aatctctttt tactgcgacc agctaaataa aggcaatacc gaaatacatt atgccgaaag
     9121 taacgataaa catgccttaa taacatatac accccgagaa ggcttcgtta ctccctacgc
     9181 gggtattggt tttaccacta ggcatagaaa cgccctttgg gacgctagtg aatatgatta
     9241 tataaatatc caggtagaga ccaccagatc aagagcccta cgcctgttta tttacacaca
     9301 tatagatgga atctcaaaaa aagggaatgt acactcgtat ctattcaatt tgcaagaaat
     9361 tcccgtttgg aaacaggaaa aaaactacca gataaaactt accgaattta acgtacctga
     9421 atggtggtac aacatgaaca atattgtacc aaacgacgag aaagtttaca gagattattc
     9481 aaaaactaga agcatagaga ttcaaaacgg tacgattgcc caacttgact ccaaagatac
     9541 gatagtagta aaaagaattt ttttctctcg atcaaaaaaa aacatactga tatactggtt
     9601 tgcagccaat tttatatatc tacttatttt tttatcgata caatatttca ggaaaaaaat
     9661 ggataggcct gcggcagcta aacgcaacgt ttcggaaaag gagagagaag agctggaaaa
     9721 aataacagac tatatcggaa accactatat ggatagctct cttaccgtaa acatggtatc
     9781 acaagccaca gaagtatccg cttataaggt atctattctg ttgaaagagg agtttacact
     9841 ctccttttca caatatttga atacgataag acttactgaa gccaaaagac ttttacagac
     9901 aacagaatta aaaatctcgg aaattgcgtc aatgaccggt tttggctcta taaaccactt
     9961 taacagaatt tttaagctta ctgagaacag aacaccggca gaatacaggc aatttaattc
    10021 ctaatcatgt aactaattta taactaagtc agtttttgta ttcaagaacc gtttttcttg
    10081 gataaaaccc catttttttg taaaacccaa ataccgactc atgcccttct gcaacggtaa
    10141 cctgataacg gttacaatct gctgaccgga accaggagag agcgttgttt acaagttttt
    10201 caccaaatcc gtgtgatcga tactctgaat caataaacaa cgactggata tcacctatct
    10261 cattggttat tgtagatata caataaccaa tgatttcgtc tttaatttgc acaatttcga
    10321 tacgaatata tttatcttca atggattcaa aggcctttat acgctgatca aaggtaaaag
    10381 attcgaaata gtttttgaaa tttacagaat cattgtagtg gattttgttc agcttttccc
    10441 aaagttcacg tattaaagaa aattgctgtt tatcaattaa gcgaaatatg gtttccatat
    10501 tatactccta tttttatgtt atttgttgat aaagaatata aactcttcga atagatccct
    10561 tacccaagta tcagtattaa caaaatttat aatattgacc ttaaataagc tgtaacacat
    10621 tttaatggaa catcaaaaga tttgtctcca ttaattacca gatgggaata ccttcgtaat
    10681 ggataaatgt ctgattcgaa atatggttcc gcctgattgt aaaacttgtg attaataaat
    10741 gaatcgtcgt gtttcataat ctgtgaatat attttataac gtctcttata tcttgtttgt
    10801 aaatccgtat caataaaaat atttaatgtg ctactgttta caagcttctt atcaacaaat
    10861 gaaaaaatgc cttcgactat aagaacccga cagcttttta tactcatgat ctttgaacgg
    10921 ctatgcgttt taaaatcgaa acattcaagt tgaaaagcct tcccacgctg tatgaggttt
    10981 acagtctcaa aaaacaactc attatcgaac gcggctgggg tgtcaaaatt atagtcgtat
    11041 agattgcaat tttgaggaat ttctttataa aaaagatctg ttgacaagca atcacaacta
    11101 acaccacttt cgacaagcat ctgttttaac cgttcagaaa atgtactctt tcccgaacag
    11161 cttcccccat taacaagtac aagcatttac aaccgtccaa tattacattg aatttattag
    11221 cattcagata tttaatttga cattattaag aaaccattgc aaattttaaa agaattttat
    11281 catgaatcga aaatcacaat aaaaaactca ttacaatttt gacacaagga gttaaaaatg
    11341 tcaacatctg aaaacactct cagcctctct tctgtgccca atattttaac agaaaccgct
    11401 ctaatcgcaa tagcaatcgc gcttccagtt gtgatgcacc ttattcccgg tataaacgta
    11461 ttcgcgacac ttcccatgca ctggacaatt tttattgcag gactacttta cggctggaaa
    11521 ggcggattga tcgcaggagt atcagtgccg actttaagtt ttgcgttaac cggaatgccc
    11581 gttccctttg tacttccatt aatgacagtc gagctgtctc tttatggttt tgtaacaggt
    11641 ctcttgagtg aaaagactat gcttaaccgc cacttgatag ttctgatttc aagtatagct
    11701 ggtcgtgccg gttttgttat actggcacta cttttaagcc gaactgattc ggccacacaa
    11761 tttgcgataa actcttttgc tcccggcata atagctgctt ttataatcat tgcttgcacc
    11821 cctgttattt caaaaattgc agccaagaat ttaaggtaaa aaagctatcc ccgccattaa
    11881 ggcggggaat tgtcactttt caaaactgta acagcggctc caagcaagcg actcttgcgg
    11941 cttgagatct atttcaatga aaaattcagg acttatgaca tgtttcattc cccaaagagc
    12001 aaaacgagat acattaaatt tttcttcaca gcctacagat atccctgact cctgatgtgt
    12061 taatttccaa tgaaattcac ccgaatcatc tattttttca gtggcgcagt aaagccatga
    12121 atcaggcgcc ccatccgggc ttatagaatt ttttccatgc tgaacaacac ctttggactc
    12181 actaagacga acctctcgat tcatgcttaa caaataactt gcatcaacag gattctcccc
    12241 aagttttaag aaattatggc aatattcatt ggtaattatt cttttctctc cgctattttt
    12301 taaactggta tctataacaa gatggttttc attgatactt atagagcggt ctagaatcca
    12361 accgtagccg ttaagtatcg gtgattgctg agaaaagtgt acattttcgt cctgtttgac
    12421 tgaaatatcc acaggcttta tctcgtaatc atggaaaaaa tcgtaatcat cttgagactc
    12481 tcttttaaga aatcccacac ctattttagg gaaaaattca cccggctttg cctcatcgta
    12541 tccgacagcc ttattgatgc caaattcaaa ggtcaggcca gttccctcgg ttcccaaatt
    12601 gtcaccagtt gcctctttag agcaaaaatt cacattatca tcaagaataa cgctgcttac
    12661 tattgccccc caatcaaagc gggaacgacg ataaacactg cctggtcgtt gtagggtaac
    12721 agttaatctt ttatttttaa ttatcacgga aatctccttt tactcgattt gaaatgttta
    12781 attttatccc aaaaaagaca ctattttgaa aagaaaacta tagcacaaga aataaagcag
    12841 catgcaataa tccttaacta atttttgaaa caatacgata gtaattatat gaatatagtt
    12901 aaaaaaactc tacttataat gctttttgta ctgacatcgt ttacaattgt ttctgaagat
    12961 tatcagtacg attatttttc gggagatgat ccggaccggc aaaaatatgt tcctttctcg
    13021 gattatatac ccaaagtaag aaagcattat cagacagtac cacattatct cgaagattat
    13081 tatcttcttt atggcatgaa acagcactac aatgaaaact cgttgcggaa aaacattgcg
    13141 atgttgcaaa cagcaatgga gtgcaagttt agacacccca tttacgcact atgcaaaata
    13201 gaaaccgaag acgaatacta taaatacaga aaccttatgt ttatgcatct gaacctgctt
    13261 attatgcgca atcatatgaa aatcgcagtc aggtatgaca agcaaacaat acggttttac
    13321 gatgccgat
//
LOCUS       DAOTGZ010000059        12981 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_3064
            NODE_5747_length_12981_cov_4.909717, whole genome shotgun sequence.
ACCESSION   DAOTGZ010000059 DAOTGZ010000000
VERSION     DAOTGZ010000059.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868088
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 12981)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 12981)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:22:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,785
            CDSs (total)                      :: 2,751
            Genes (coding)                    :: 2,732
            CDSs (with protein)               :: 2,732
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 12 of 19
            Pseudo Genes (internal stop)      :: 5 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12981
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5747_length_12981_cov_4.909717"
                     /isolate="AMR_MDS_3064"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..234
                     /locus_tag="P5123_05610"
     CDS             <1..234
                     /locus_tag="P5123_05610"
                     /inference="COORDINATES: protein motif:HMM:NF012267.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HRX15768.1"
                     /translation="NLYIDDKCNLCGTCALVCPVDNISVTDSVNFNHKCESCFACLHN
                     CPQKAIHHKKEKSSSRYREKSISLNDIIESNNI"
     gene            complement(286..1287)
                     /gene="pta"
                     /locus_tag="P5123_05615"
     CDS             complement(286..1287)
                     /gene="pta"
                     /locus_tag="P5123_05615"
                     /EC_number="2.3.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013657318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008959 - phosphate acetyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006086 - acetyl-CoA biosynthetic process
                     from pyruvate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate acetyltransferase"
                     /protein_id="HRX15769.1"
                     /translation="MSMLQNFKQRAKSLNKTIVLPEGTDGRTIKAAALITEEKIAKVI
                     VLGDPKEIAELASEQGVTTDNFQIVDPKNYDRRKELVEKLVELRKSKGVDIEKAEQLL
                     NDPLYFGVMMVKCDIADGMVAGAINATANVLRPSLQILKTAPGTKLVSAFFVMIVPNC
                     DYGADGGFIYADSGMVQNPSSEELAHIAINAAKSFKLLFQKEPVVGMLSHSTKGSAAH
                     PDVDKVVEATRIAKELDPELALDGEFQLDTAIVPSVGKAKAPESSVAGKCNVLVFPDL
                     DAGNIGYKLTQRLAKAEAYGPVTQGIAKPVNDLSRGCTAEDIVGVAAITAIQADASK"
     gene            complement(1609..2265)
                     /locus_tag="P5123_05620"
     CDS             complement(1609..2265)
                     /locus_tag="P5123_05620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011235664.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin III family protein"
                     /protein_id="HRX15770.1"
                     /translation="MKTEKKIKRSQTSKEEFFNFLTHGTAALLSIAGLVFLVVGAAIG
                     GDPWRIVSMSIYGASLVMLYCASTFYHFAKDSQKKSYLKIFDHTSIYFLIAGSYTPYV
                     LVTINGTIGWVIFGIVWGITIIGIVFKFKYVGQFKKLSLALYLGMGWVIIFAIRDIVE
                     ALPSGGLLLLVLGGVAYSVGTIFYAKDSKPYFHAIWHLFVMAGSIFHYMSISMYVLPK
                     "
     gene            complement(2262..2717)
                     /locus_tag="P5123_05625"
     CDS             complement(2262..2717)
                     /locus_tag="P5123_05625"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRX15771.1"
                     /translation="MKRIVLVLAVLTVMSCKSAPVKEPAVVQEDTVVAGTGQVEIVVR
                     DTGIMDPENDRRSYYRVFIDKIEVGRTEIGLESQIKKFATTTEPNSHLLRIEKYVLDE
                     KNDRYVKVNNIDQPRPNFFYFDNTDKRNLKIEIIHTVDTHHAEYRLIKR"
     gene            complement(2753..4300)
                     /locus_tag="P5123_05630"
     CDS             complement(2753..4300)
                     /locus_tag="P5123_05630"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRX15772.1"
                     /translation="MYRIKNIFLLVLIFSIYNCSRNGSENEPNNSFDTSTPVTIGKNF
                     AGSIESAEDMDFFKLDVDRETRISISLSQLKGINHSITIFRDLNGKQTVVKRVDDSRK
                     SSPEKMPCLGLIPGTYYIVVSHGERDEKKGSDQQYYLLIDELVQDENFEFEPNDTPRL
                     ATPVTSGNSISGFHSPSFNKLNKSSENQYREYDYFSFKVSSQEAGRYLADISVSGVPG
                     VDVVLAMYNPSGHKINSSDVGIIGTAESIIGQGLSDAGNYIIEIHAKDFASNNDTPYT
                     LNVNISEFDSSFEMELNNSVESANVLRGSMIKGRIFPEGDEDYFVYIPSRPGFVDITL
                     VPTPEINGRMALYNAEGDIVFEIDNHEAGMTENFPAFFTGEKLYIRVTAFSGEVDSEK
                     YYSLYVKESAQQESVELEPNDEKDRATFIDKEIMYGYSSYKGDRDFYLIETKLRAEKI
                     IRITGVEQAHFEISVTDNLGYVINSYELKDGNVLEITELVDKRAYLIIDAKEAVFDKP
                     YIIETVN"
     gene            4507..6636
                     /gene="glgX"
                     /locus_tag="P5123_05635"
     CDS             4507..6636
                     /gene="glgX"
                     /locus_tag="P5123_05635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020610862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004133 - glycogen debranching enzyme
                     activity [Evidence IEA];
                     GO_process: GO:0005980 - glycogen catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycogen debranching protein GlgX"
                     /protein_id="HRX15773.1"
                     /translation="MITDNSRVTLPGYPLPYGATLDAKGTQFSIFSSNALSVTLVLYD
                     GTETDSQFEEYRLDPLHNKTGDIWHIWIEGVKEGQLYGYRIDGPYFPEKGHRFNRNKL
                     LLDPYARAITGNSVWNIDDARGYDYTHDTDIFSTKDSGPVVPKCIVINHDFDWFDRPL
                     NRPMHEMIIYETHVKGFTFHPSSGSDCGGTYKALTQKIPYLKELGVTAVELLPVHEFD
                     MNENFRVNPETGEKLKNYWGYSTISFFAPCSHYSSSTKNGSQIYEFKEMIRDFHNAGI
                     EIILDVVFNHTAEGDHRGPTLSFRGIDNTVYYILEENKAMYKNFSGCGNTFNCNHPLV
                     RDFILDCLRYWVIEMHVDGFRFDLASILGRAQDGTILSNPPLIERIESDPILRNTKII
                     AEAWDAAGAYQVGKFPGRWAEWNGKFRDDIRRFWRGDDKSVGAFATRISGSSDLYSHN
                     NRSPLHSINFITSHDGFTLNDLVSYTHKHNRNNGENNNDGDNHNLSYNYGIEGNEVTP
                     FIESIRTKQIKNFIFTLLISQGVPMITAGDEFRRTQKGNNNAYCQDNEISWIDWNLID
                     NNHAIFEFVKNMITFRKNHPVLRKRHFASSENPQDGNSEFSWHGTRPFLPDWSDNCKI
                     IGCMLNGEAAVSENKKRDSDIYFVCNASLFNYRIELPESPSGRQWRLVTDTADKEHTF
                     FPDGNISVLTDNMYRVKKQSAILFIAD"
     gene            6738..9044
                     /locus_tag="P5123_05640"
     CDS             6738..9044
                     /locus_tag="P5123_05640"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRX15774.1"
                     /translation="MNILLIILLLAIFLIPIWILFGKPSSSKTISESSKDSSLLDKID
                     FKDSMSDEFPDDLPVPTPRSTSPDEITKIGSDFKLPFNSSNIISQDSPFSIYSGTLDN
                     ADYHTQHGDFASAIDLYEGLIQRISAPDIRKKINENIDYLNNYQQISATREKERRFKE
                     ADSDSNEIKLSLGGKDYSPQNVKIDISPTSLSTDFNMDSIVNSINDQITKLNPALQQS
                     SEKIDEIMQYKDELQQLKYELRSLYDLKEESHRIREDKIRNEIEELKSVRESLIKPQT
                     IADSSKQDNEIEKLKSEIEELKQKQATQSLQKVSSQSIEEIHKIDVTGLPELPEHSIK
                     ITDTPEPKEQKVKITEMPDKGEIEALKLKVESISNDLTVRNLDSSANSLHQKELEELR
                     NKVSFLENEKLSEISKEKQEENNLLRSQLEIMQKQMEELKNGSDQAKALHELKERIQN
                     FENQNLSKSLDHEQMQRIVSEIESLSQSAKLSKDIEEKIEKLIQNQLSSGLEGITHSK
                     IDELTSQISDLKGQLASVSSPNQTMPPSSQAQPATSTSLPQDSSIDISKKSDKADQSK
                     QNGPVTTQDTNLQISAEEKEENKKDDFQTLEEYINGPTYEEPSEDEILDKILAESAKN
                     KEKDYEIRGTDDSKDISDDFDLSKLFAKPSAIDTKDEEFYNQFTERNKPRVKKELPIL
                     HVSYRFDKLPEISSLSKESNVIATTFYKYKDLLEKANEYIKRRRVKDALNYYQVVMDQ
                     DIPDEFKVMLKQNIDDLKEYLEKYMLNM"
     gene            9182..9871
                     /locus_tag="P5123_05645"
     CDS             9182..9871
                     /locus_tag="P5123_05645"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013053.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isochorismatase family cysteine hydrolase"
                     /protein_id="HRX15775.1"
                     /translation="MKTALIIVDMQKYYLEKSATFFSYYEHLYPGSMDYIKDRSRETV
                     IPNIQKLIEYFRNTNHPVYFLRLCGCKKDRSDLHRFFKKEHDNAYKKGYKDLYPLCDQ
                     KDSEIIDQLKPHKNDRIICKTTFSPFTLTDFEDTLRKDDITTLVFTGLATSQCVETTA
                     RDASDRNFDIIHIRDAQADYNEDNHQISLFNSRSVCGGWVYDTDEFYSDQDKILHAIE
                     INDRSSATKYK"
     gene            9892..12261
                     /locus_tag="P5123_05650"
     CDS             9892..12261
                     /locus_tag="P5123_05650"
                     /inference="COORDINATES: protein motif:HMM:NF015587.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhjD/YihY/BrkB family envelope integrity
                     protein"
                     /protein_id="HRX15776.1"
                     /translation="MAIKKISKKSTSSTNLKKKDISNKTIKKSKKNFFKKSVELILKF
                     LKNITDEEYITDITYGRISRAIISTIKVFIASFRKFIDDDCMTKATAITYTTLVSLIP
                     TLMVIMTVVSMFMYKGVEAQKDEIFDQITRFLAEYNLQRLNITPLLNAISGLLDNSKR
                     IGGIGAVVLIFSATAILRTIEQALNKIWKIKKQRPFFNKFIYYWAALTLGPLMLFVGT
                     TLATQLSASFSEPDYNALLQNNGKYWIVGSKATAGYKISPLAPLIPISLSTVDFENQR
                     NYNFSHDDGEFIIDNSIIIDENLFKKYSFNDINIIGSQIIICGDNGILLSSTNYGASW
                     KITKLSFFNLRKIHMFDSSKGIILSDQGTMLRTDDGGINWNLLSIPNISSNLHDVAFN
                     NNYGIVVADNGFILESFDGGDSWIAMQLNKARLQNDFVALNSVAIKGSSILIVGSGGM
                     ILKSEDRGKTFSSRHYKNDDYSAVFLEDNSNYYIASRKGKLIYTQDDGANFEILLKNS
                     KKINTIIASSDQIILSGKTGLLLFTNDKGFNWSGNLNRSILFYLLNFVAPFAVIWVLF
                     LLMYLVMPNTRIPFHAASIGASFTSAVWVGFIYIFIIYIKSFANGTFAIYGALAAFPL
                     TLLLIYASNNIMLFGAEIAYTLMYPTTYRNLKRKIAIRDYIQVINGVKILYYIFNKFE
                     SGKGGSDHQEILKLCSNNITETDYYIDLFKSKKLILMTDNSYIPATASVNIQIATIVE
                     MIHNFTFDSSSAHNDPVKRHLGAKFKKMSQSFNTILGKQTLADLINDKG"
     gene            complement(12286..>12981)
                     /locus_tag="P5123_05655"
     CDS             complement(12286..>12981)
                     /locus_tag="P5123_05655"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRX15777.1"
                     /translation="ITDRRGDTWLNNSLQFQQMRMQAAKLSDGSSSKIAPLIKSNRRS
                     DFIISFYDEPKFIEKPVDKTEKDKNADADEKSDKTDAGEKSLSRDGIDWSAIPDKKGI
                     NLDNAIYYSNSDYFSFNTKKFSNFEYFRRMKQKIAGNWYPPMMANSLTTGYTPGHVRV
                     KIMASEEVVTYFILNRNGDVQKVVLLESKGNKSLHDSCVDAIKHSKSFGPVPEDIKGD
                     KIVIPFIFGYYVR"
BASE COUNT         4351 a         2536 c         2181 g         3913 t
ORIGIN      
        1 aatctatata tcgatgataa atgtaatctt tgtggcacat gtgcacttgt gtgtcccgtt
       61 gacaatatct cggtgacaga cagtgttaat ttcaatcata aatgtgaatc atgtttcgcc
      121 tgtctacata actgccctca aaaagcgata caccataaaa aagagaaaag ttcatcacgt
      181 tacagggaaa agagcatttc ccttaacgat attatcgaaa gcaataatat ataaataccc
      241 gtgtaagcgg gtatttatat taacatgtat aaacaaatta tattactatt ttgaagcgtc
      301 agcctgtatt gcagtaattg ctgcaacgcc gacaatatcc tcggccgtac agccacgaga
      361 aaggtcgtta acaggttttg caataccctg ggttacaggt ccatatgctt cagcttttgc
      421 caagcgttgc gtcaacttat agccgatatt accggcatcc agatcaggga aaaccaatac
      481 attacattta ccagcaacac tgctctcagg cgcctttgct ttaccaacag aaggtacaat
      541 tgctgtatct agctgaaatt cgccatccaa agccagctca gggtcaagct cttttgcgat
      601 acgagtagcc tctacaacct tgtcaacatc gggatgagcc gcagatccct ttgtcgaatg
      661 tgatagcata ccgacaacag gctctttctg aaacaatagt ttgaacgact tagcggcgtt
      721 tatggcaata tgcgccaact cttcagagct tggattttga accataccag aatcagcata
      781 aataaatccg ccatcggcgc cataatcaca attaggtact atcataacga aaaatgctga
      841 tacaagtttt gtaccaggtg ctgtttttag aatctgcaaa gatggcctta gaacattagc
      901 tgttgcgtta attgctccgg caaccatacc atccgcgata tcgcatttca ccatcataac
      961 accgaaatat agaggatcat taagtagctg ctcggccttt tcaatatcta ctcccttaga
     1021 ttttctcagt tctacaagtt tttcgacaag ttcttttcga cgatcatagt tttttgggtc
     1081 aactatttga aaattatctg ttgttactcc ctgttcggac gcaagttcag caatttcttt
     1141 tgggtcaccc aacacaatta cctttgcaat tttttcttca gtaataaggg ctgccgcctt
     1201 aatggtccgg ccgtccgttc cttctggaag cacaattgtt ttatttagag acttagctct
     1261 ttgcttgaag ttctgaagca tactcatata ttatctcctt aatgtttgat aatagggaac
     1321 ttaaagtcat cttctaacaa taggatactt cgaaaaatta ccattttcca gagggtaaca
     1381 tcgtttaatt gcctattctc aaaggaattt ttagcaagtt ccttgttgac aagttaatta
     1441 ttagaaatta tctatagaat aattagaatg tcaaactttt taaagtcact gtctataaac
     1501 tctaaatttg gtcaataata taaatacaaa aaaccgcgac ttaaataagc cacggttaga
     1561 cgatcaaccg atttattcga aaccggattt gaatcagatt aacaggattt attttggaag
     1621 aacatacatg gagatactca tgtaatgaaa tatactcccc gccattacaa agagatgcca
     1681 gatagcgtga aaataaggct tactgtcttt ggcataaaag atggtgccga cactataagc
     1741 gactcctccc aaaaccagca gcaataatcc accagagggc aaagcttcca caatatccct
     1801 tattgcaaaa attattaccc atcccatacc aagatacaat gcaagggaaa gctttttgaa
     1861 ttgtccaaca tacttaaatt taaagacaat tccaataatg gtaatacccc atacgatacc
     1921 gaaaataacc catccaatag ttccgtttat tgttacaaga acataagggg tgtaagaacc
     1981 cgctatcaaa aaatagatag atgtatgatc aaatattttt agataactct tcttctgtga
     2041 gtctttggca aaatgataaa aagtcgatgc gcaataaagc ataacaagtg acgctccata
     2101 aattgacatc gaaactatac gccatggatc acctcctatt gcagcaccga ctaccaaaaa
     2161 aacaagcccg gctatactta gcagggctgc agttccatgt gttaaaaagt taaaaaattc
     2221 ttcttttgat gtttgagaac gtttaatttt tttttctgtt ttcaccgttt aatcaacctg
     2281 tattccgcat gatgcgtatc tacagtatgt attatttcta tcttcagatt tcttttatcc
     2341 gtattatcaa aatagaaaaa atttggtctg ggctgatcta tgttgttaac tttaacataa
     2401 cgatcattct tctcatccaa aacatacttt tcgatacgta atagatgact gtttggttcg
     2461 gttgtggttg caaatttttt tatttgagat tccagaccaa tttcggttct acccacttct
     2521 attttatcaa taaaaacacg gtaatatgaa cggcgatcat tttcaggatc cataattccc
     2581 gtatctcgca ccacaatttc tacctggcct gtgcctgcaa caacagtatc ttcttgtaca
     2641 acagccggtt ccttaactgg agcgctttta caagacataa ctgttaaaac tgccaaaacc
     2701 aatactattc ttttcatcgt aacccctgat ttcatattaa tattaatcat cactaattca
     2761 ccgtttcaat aatataaggc ttgtcaaaca cagcctcttt tgcgtcaata atgagatacg
     2821 cacgcttatc gacaagttct gtaatttcaa gaacattacc atctttcagt tcataactgt
     2881 ttattacata tccaagatta tcagtaacag atatttcaaa atgggcctgt tctacacctg
     2941 taatccttat tatcttttcg gctcgcaact tggtttcaat cagataaaag tcacgatcac
     3001 ctttgtatga agagtaacca tacataatct ctttatctat aaaagtcgca cgatcttttt
     3061 catcattagg ctctagctct actgactcct gttgagcact ctctttaaca taaagtgagt
     3121 aatatttttc tgaatcgacc tcgccagaaa acgcagtaac ccttatatat aacttctctc
     3181 cagtaaagaa ggctgggaaa ttttcagtca tacccgcttc atgattgtca atttcaaata
     3241 ctatatcacc ttcagcatta tagagagcca tacggccgtt aatttcagga gtgggtacaa
     3301 gagtaatatc aacaaatccc ggtcttgaag gaatataaac aaagtaatct tcgtctcctt
     3361 ccgggaatat gcgtccttta atcattgatc ccctcaagac atttgcactt tcaacactat
     3421 tatttaactc catttcgaat gaagagtcaa actccgatat gtttacgttc aaagtataag
     3481 gtgtatcatt gtttgacgca aaatcttttg cgtgtatctc tataatgtaa ttaccggcat
     3541 ctgataaacc ttgcccgatt atgctctctg ctgttccaat tataccaaca tcagagctgt
     3601 taatcttgtg acctgatggg ttatacattg ctaagacaac atctacgccg ggaacacccg
     3661 aaacagaaat atcagcaagg taacgccctg cttcttgtga gctaacttta aaactgaaat
     3721 aatcatattc acgatactga ttctcggatg atttattaag tttattaaaa cttggcgaat
     3781 ggaaaccgga tattgaattt ccactcgtta cgggcgtagc aagtctcggt gtatcattcg
     3841 gctcaaattc aaaattttca tcttgaacaa gttcatctat taacaagtag tattgttgat
     3901 cactgccctt tttctcgtcc ctttctccat gagacacaac aatataatat gtacctggta
     3961 ttaaaccaag acaaggcatt ttttcaggag acgatttacg agaatcatca actcttttta
     4021 ctacggtctg tttgccattt aaatctctga aaattgttat cgaatgattt atacctttaa
     4081 gctgcgagag agaaatcgat attcttgttt ccctgtcaac atccagtttg aaaaaatcca
     4141 tatcttcggc actttcgata ctacctgcaa aatttttacc aattgttacc ggagttgaag
     4201 tatcaaaact attattgggt tcattttcac tgccatttct gctgcaatta tagattgaaa
     4261 agattaaaac caaaagaaag atatttttta ttctgtacaa atcgatacac tcctgatacc
     4321 atcaaacaag aaaatttatt accgtgataa ctattttttg aactttattt aacgtcaact
     4381 cataattgca atataaaaca ccatgatgtt acttttaaag cctgacaaag ccaaattatt
     4441 gttgaaatat aagcctattg tattcattat aaggttgatt acttatcctg acggagataa
     4501 ttcattgtga ttactgataa cagtcgcgtg accctgcccg gttacccact tccctacgga
     4561 gcaactcttg acgctaaagg cactcaattt tctattttct caagtaacgc actttctgta
     4621 acccttgttt tatacgatgg aacagaaact gattcacaat ttgaagaata taggcttgat
     4681 ccgctacata ataaaacagg tgatatttgg catatatgga tcgaaggcgt caaagaggga
     4741 caactttatg gatacagaat agacggtcca tatttccctg aaaaaggaca tcgatttaat
     4801 cgaaacaaac ttttgcttga tccatacgcc cgagcaatta ccggaaactc tgtttggaat
     4861 attgatgatg cgcgtggata tgactatact cacgataccg atattttttc tacaaaagac
     4921 agtggccccg tagtaccaaa gtgcatagta ataaaccatg actttgactg gtttgaccgt
     4981 cctttaaaca gacccatgca cgaaatgata atttatgaaa ctcatgttaa agggttcacc
     5041 tttcacccat catcgggttc cgactgcggc ggaacatata aagctttaac tcaaaaaatt
     5101 ccatacctta aagagctcgg agtaacagcg gtagaacttt tacccgttca cgagtttgat
     5161 atgaatgaaa acttcagggt taatcctgag actggcgaaa aattaaaaaa ctactggggt
     5221 tacagtacaa tctcgttttt tgctccttgc tcccactact cctcttcgac caaaaacgga
     5281 tctcagattt acgaattcaa agagatgata agggattttc acaatgccgg aatagagatt
     5341 attcttgatg tggttttcaa tcacacagcc gagggagacc atcgaggtcc cacactctct
     5401 tttagaggaa tagataatac cgtgtactat attcttgaag agaacaaagc catgtacaaa
     5461 aacttttcag gttgtggtaa cacctttaat tgtaatcatc ctcttgtcag ggattttatt
     5521 ttggactgct tacgctattg ggtaatcgag atgcatgtag acggtttccg atttgatctt
     5581 gcatcaattt tgggacgtgc acaggacggc actattttaa gtaatcctcc ccttatcgaa
     5641 agaatagaat ccgatccgat acttcgtaac acaaaaataa tcgccgaagc ttgggacgcg
     5701 gccggtgcgt atcaggttgg taaatttcct ggtcggtggg ccgagtggaa cggaaaattt
     5761 cgtgatgata ttagacgatt ttggcggggt gatgataaaa gcgttggcgc atttgcaaca
     5821 cgaattagcg gcagctctga tctttacagc cacaataatc gatctccgct tcatagtatc
     5881 aattttatta ccagccatga cggttttact ctgaatgatc ttgtctctta tactcataaa
     5941 cataaccgaa acaatggtga aaacaataac gatggtgaca atcacaacct ttcctacaat
     6001 tatggaattg aaggtaacga agttacacct ttcatcgaat ctataagaac aaaacagatt
     6061 aaaaatttta tttttacatt gcttatctcg caaggtgttc ccatgatcac cgccggtgat
     6121 gagtttagac gaacacagaa gggcaataac aacgcttact gtcaagacaa tgaaatatcc
     6181 tggatagatt ggaacttgat agataacaac catgcaatat ttgaatttgt aaaaaatatg
     6241 ataactttca gaaaaaatca tccagtttta aggaaaagac acttcgcaag cagcgaaaat
     6301 ccacaagacg gaaattccga attcagctgg cacggaacac gccctttttt gccagattgg
     6361 tctgataatt gcaaaataat cggctgtatg ctcaatggtg aggccgccgt ttccgaaaat
     6421 aagaagaggg attcggatat ctattttgta tgcaatgctt cgctttttaa ttaccgcatc
     6481 gaactgcccg aatccccatc gggtcggcaa tggagactcg taacagatac tgccgataaa
     6541 gaacacacat tttttccgga tggcaatatt tcagtgctaa cggataatat gtatcgtgta
     6601 aaaaaacaat cagcaatact gtttattgcc gactaaagta attatttcag tgtcaatttt
     6661 aattactgcc taaactcaaa gcgaaaccta tttattattt aaagtttcaa ttatttattt
     6721 caggattata acggtttatg aatatattac tgattatatt attactcgca atttttctaa
     6781 ttcctatttg gatacttttt ggcaaaccat catcttcgaa aactatttcc gaatcctcta
     6841 aggacagctc ccttcttgat aaaattgatt ttaaagattc catgtccgat gaatttccag
     6901 atgatttgcc agtacccacc cccagatcca cctcaccaga cgaaataaca aaaataggat
     6961 ccgactttaa actacctttt aattcgtcaa atataatttc acaagatagc ccattctcta
     7021 tatattccgg aactcttgat aacgccgact atcacactca acacggtgat ttcgcatctg
     7081 ccattgatct gtatgagggg cttatacaac gcataagtgc gcccgatatc agaaaaaaga
     7141 ttaatgaaaa tatagattac ttaaataact atcagcagat ttcggcaaca cgcgaaaagg
     7201 agcgcagatt caaggaggca gactctgatt ctaatgaaat aaaactatcg cttggtggaa
     7261 aagattattc acctcagaat gtaaaaatag atatatctcc cacatcattg agcacagatt
     7321 ttaatatgga ttccattgtt aattcaatta acgatcagat caccaaactt aatcctgcct
     7381 tacagcaaag ttctgaaaaa attgatgaaa ttatgcaata caaagatgag cttcagcaat
     7441 taaagtatga gttgcgctct ctttacgatc tgaaagagga atcacatcgt atacgcgaag
     7501 ataagattcg caatgaaata gaagaattga aatctgtcag ggaaagtctt attaaacctc
     7561 aaactatagc tgacagctcc aagcaagata atgaaataga aaaacttaaa agcgagatag
     7621 aagagctcaa gcaaaaacag gctacgcaat ctttacaaaa agtttcttca caatcaattg
     7681 aagaaatcca taagattgat gttaccggac ttcctgagct gcccgagcat agcattaaaa
     7741 ttaccgatac tccagagccc aaagaacaga aagttaaaat tactgaaatg cccgacaaag
     7801 gagaaataga ggcacttaaa ctcaaggtcg aaagcatatc aaacgatcta actgtaagaa
     7861 atctagactc atccgcaaat tctcttcatc aaaaagaatt agaagaacta agaaacaagg
     7921 tttcttttct tgaaaatgaa aaactctctg aaatttccaa ggagaagcag gaagaaaaca
     7981 atttgcttcg atctcaactt gagattatgc aaaaacagat ggaagagctc aaaaacggct
     8041 cagatcaagc aaaagcatta catgagttaa aggaacgcat ccagaatttt gaaaatcaaa
     8101 atctttccaa atcccttgat catgaacaga tgcaaagaat tgtaagtgaa atagaaagtc
     8161 tatctcaaag tgcaaaactt tcaaaagata tagaagaaaa gattgaaaaa cttattcaaa
     8221 accagttatc atctggtctt gagggaatta ctcattcaaa gattgacgaa cttacttctc
     8281 aaatcagcga tcttaaagga cagcttgcat ctgtgtcttc accaaatcag actatgcccc
     8341 cttcgtcaca agcacaaccc gctacttcta catcgttgcc gcaagactca tcaattgata
     8401 tttcaaaaaa gagtgataaa gctgatcaat caaaacagaa tggccctgtt acaacacagg
     8461 atacaaatct tcaaatatca gcagaagaaa aggaagagaa caagaaagat gattttcaga
     8521 ctcttgagga gtacattaat ggcccaactt atgaagagcc ttctgaagat gaaatactag
     8581 acaaaattct tgccgaaagt gccaaaaaca aagaaaagga ttatgaaata aggggtaccg
     8641 atgattcaaa agatatttca gatgattttg acctttcaaa gcttttcgcg aaaccatctg
     8701 ctatagatac caaggatgaa gaattttaca atcagttcac ggaacgcaat aaaccgaggg
     8761 taaaaaaaga gctgcctata ctgcatgtga gctaccgttt tgacaaatta cccgaaatat
     8821 catctttgtc aaaagagagc aatgttatag ctacaacatt ttacaaatac aaggatcttc
     8881 ttgaaaaagc aaacgaatat attaaaagac gtagagtaaa agatgcctta aactattatc
     8941 aggttgttat ggatcaggat attccagacg aatttaaagt catgttaaag cagaatatag
     9001 atgatcttaa agaatacctt gaaaaataca tgcttaatat gtaacaggat atttgaaaaa
     9061 attacagctc acagtatata ttaacttata caaaagataa ttttccagtt gtcactcaaa
     9121 agatttatga caaaacactt aacaaatagt gcaatatttt tagattatta aaaggataaa
     9181 tatgaaaaca gctctgatca tagttgacat gcaaaaatat tatcttgaga aaagtgccac
     9241 attttttagc tattatgagc acctttatcc tggatcgatg gattatatta aagatcgatc
     9301 ccgagaaaca gtaataccaa acatacagaa gcttatcgaa tatttcagaa ataccaacca
     9361 tcctgtatat tttttaagat tatgtggatg taaaaaagac cgtagcgatc tgcatagatt
     9421 tttcaaaaaa gaacatgata acgcctataa aaaaggttat aaggatcttt atcctctttg
     9481 cgatcaaaaa gattctgaaa taatcgatca acttaaacct cataaaaacg acagaatcat
     9541 atgcaaaaca actttttcac cttttaccct tacagacttt gaagatacac ttcgtaaaga
     9601 cgatattaca actcttgtgt ttaccggatt agcaactagc caatgtgtag agacaacagc
     9661 aagagacgca tctgatcgca atttcgacat tatacatatc agggatgccc aagccgacta
     9721 caatgaagac aatcatcaaa tatcactctt taactccaga tctgtttgtg gtggatgggt
     9781 ctatgacaca gacgaatttt atagcgatca agataagatt ttacatgcga ttgaaataaa
     9841 tgacagaagt agcgcaacca aatataaata gtcgtgttat tggagaagaa aatggcgatt
     9901 aaaaaaatct caaaaaagag tacctcttct acaaatttga aaaaaaaaga tatatctaat
     9961 aaaactataa aaaaatcgaa aaaaaacttt ttcaagaaat cagttgaatt gatacttaaa
    10021 tttctaaaga acattactga tgaagaatac ataacagata ttacgtatgg ccgtattagc
    10081 cgcgcaatta tcagtaccat caaagttttt attgcctctt ttcgcaagtt tattgacgat
    10141 gattgcatga cgaaggcaac agccataacc tacacaacgc tcgtctcttt gatcccaaca
    10201 cttatggtta ttatgactgt tgtttcaatg tttatgtaca aaggtgtcga agctcaaaaa
    10261 gacgagattt tcgatcaaat cacccgtttt cttgcagaat acaatcttca acgattgaat
    10321 ataacacccc ttttaaatgc tatatcaggt cttcttgata attcgaagcg aataggtgga
    10381 attggtgctg ttgtgttaat attttcggca acagctattt taagaactat tgagcaagca
    10441 ttgaataaga tttggaaaat taaaaaacag agaccttttt ttaacaagtt tatctattac
    10501 tgggctgctt taactcttgg tcctcttatg ctctttgtag gaaccactct tgcgacccaa
    10561 ctttctgcct ctttttctga gccagattat aacgcactat tgcaaaataa tggcaaatac
    10621 tggatcgttg gatctaaagc tacggccgga tataaaatat cgccattagc acccttgata
    10681 cctatatcac tatcaactgt tgattttgaa aaccaacgga actataattt tagccatgac
    10741 gacggagaat ttataataga caacagtatt attatcgatg agaatctttt taaaaaatac
    10801 agtttcaatg acataaatat aatcggatca caaattatta tatgtggtga taatggtatt
    10861 cttctttcat caaccaatta cggagcaagc tggaaaatta ccaaactctc tttttttaat
    10921 cttcgtaaaa ttcatatgtt cgattcatca aagggaatta ttctttcaga tcagggaaca
    10981 atgcttagaa ctgacgatgg gggaattaac tggaaccttc tttcaatacc taatattagc
    11041 agtaacttac atgatgtcgc tttcaataat aattacggca ttgtcgttgc cgacaatggt
    11101 tttattctgg aatcatttga cggtggcgat agttggatag ccatgcaact taacaaagcg
    11161 cgtttacaaa acgattttgt tgcactaaat tctgttgcaa tcaaaggatc atcaatatta
    11221 attgtaggca gcggcggaat gattttaaaa tcggaagacc gaggtaaaac attttcttca
    11281 cgtcattata aaaacgatga ctacagtgcc gtatttcttg aagataattc aaattattat
    11341 atagcttcac gaaaagggaa gcttatctat actcaagacg atggagcaaa ctttgaaatt
    11401 ttattaaaaa acagcaagaa gattaataca attattgctt catctgatca gattattttg
    11461 tctggcaaaa ccggcttgct gcttttcaca aatgataaag gatttaactg gagtggcaat
    11521 ttaaaccgga gtattctctt ctatttgctt aactttgttg ctccatttgc cgttatctgg
    11581 gtactttttc ttctaatgta cctggttatg cccaataccc gaataccctt tcacgcagcg
    11641 tcaattggcg cctcttttac ctcggcagta tgggttggat tcatttatat ttttatcata
    11701 tatataaaaa gtttcgcgaa tggaacattt gccatctatg gagccctagc tgcctttcca
    11761 ttaacactgc ttctaattta tgcatcaaat aatataatgc tatttggtgc ggaaatcgcc
    11821 tatactttga tgtaccctac aacatacaga aatctcaaaa gaaaaatcgc aatcagagat
    11881 tatatccagg taatcaatgg tgtaaaaata ctctattaca tttttaacaa atttgaatca
    11941 ggtaaagggg gatcagatca ccaggagata ttgaaacttt gttcaaataa tataacagaa
    12001 accgattact atattgatct ttttaaatcc aaaaaattaa tactaatgac agataattcc
    12061 tatatcccgg cgacagcctc cgttaacatt cagattgcaa ccatagtcga gatgattcac
    12121 aactttacat ttgatagctc aagcgcacat aatgatcctg tcaaacggca tttgggggcg
    12181 aagttcaaaa agatgtcaca atcgttcaat acaattttgg gtaagcagac ccttgctgat
    12241 ttaataaacg acaagggata aaaaaatatt agatctcgtt tagatttatc ttacataata
    12301 gccaaagata aaaggtatta ctattttatc gccctttatg tcttcaggca caggtccaaa
    12361 ggatttagaa tgcttaatgg cgtcaacaca ggaatcgtgc aacgatttat ttcctttgga
    12421 ctcaagaaga acaacctttt gaacatctcc gtttctgttt aaaataaaat atgtgacaac
    12481 ctcttcactt gccataattt tgactctgac gtggccggga gtatatcccg ttgtcaaaga
    12541 attggccatc attgggggat accagttacc tgcaattttc tgtttcatac ggcggaaata
    12601 ctcaaaatta gaaaactttt ttgtattgaa tgaaaagtaa tctgaatttg aatagtatat
    12661 ggcattgtca aggttgatac cttttttatc aggaatcgca ctccaatcga ttccatcacg
    12721 tgataaactc ttttcgccag catcagtttt atccgatttt tcgtctgcat ctgcattttt
    12781 atctttttcg gttttatcaa ccggtttttc aataaacttt ggctcatcgt aaaacgatat
    12841 aataaaatct gaacgcctat ttgattttat taggggagct atctttgaag atgaaccgtc
    12901 tgataatttt gcggcctgca ttctcatttg ctgaaactga agcgaattgt ttaaccaggt
    12961 atcaccccgt cgatcggtta t
//
LOCUS       DAOTGZ010000060        12833 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_3064
            NODE_5840_length_12833_cov_5.497730, whole genome shotgun sequence.
ACCESSION   DAOTGZ010000060 DAOTGZ010000000
VERSION     DAOTGZ010000060.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868088
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 12833)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 12833)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:22:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,785
            CDSs (total)                      :: 2,751
            Genes (coding)                    :: 2,732
            CDSs (with protein)               :: 2,732
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 12 of 19
            Pseudo Genes (internal stop)      :: 5 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12833
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5840_length_12833_cov_5.497730"
                     /isolate="AMR_MDS_3064"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..233)
                     /locus_tag="P5123_05660"
     CDS             complement(<1..233)
                     /locus_tag="P5123_05660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015678044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellin"
                     /protein_id="HRX15778.1"
                     /translation="MIVNHNMSAINANKVLKFKHWDVNKSTEKLASGMRINRSGDDAS
                     GLAVSEKMRTQIQGLRQAERNTEDGMSFVQTAEG"
     gene            complement(391..1236)
                     /locus_tag="P5123_05665"
     CDS             complement(391..1236)
                     /locus_tag="P5123_05665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002764028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellin"
                     /protein_id="HRX15779.1"
                     /translation="MIINHNMSAINANRVLKFQHWNLDKYTEQLSSGMRINRAGDDAS
                     GLAVSEKMRTQIQGLRQAERNAEDGMSFVQTAEGYLQQLHEIIQRIRVLAVQSSNGIY
                     TMEDRQLIQVEVSALIDEVDRIASQAEFNKMNLLLGDFSRVNARASMWFHIGANMHQR
                     ERVFIQTMTAQALFLKSEVGELMTLSTAESSNRSIGVLDEAIHKISKQRADLGAYYNR
                     LEIASKGLLNAYENIQASESRIRDADMAEVMVNFTKTQVLVQSGTAMLAQANEMSNSV
                     LLLLQ"
     gene            1624..2694
                     /locus_tag="P5123_05670"
     CDS             1624..2694
                     /locus_tag="P5123_05670"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013700.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M23 family metallopeptidase"
                     /protein_id="HRX15780.1"
                     /translation="MGLNPFKDADLYDLETYLCKRMVGISRNVKSVLSSFISGINRKG
                     NEKITLMFIPHSEKRIINFHISIYSIVIFSFFLISVVTTTTIMIMNHTTTIKEINRLR
                     LYGYDSQMQISHYKKEINSLHDTFQKFKPELTYLYSLTPDNYVDSLWAKGGATGDIAE
                     APVSDDEQSDFSVTVENSNTAPDIEELNIQEMGQELKTTKEVLKKIKVFLSNRKKIIE
                     NTPSIWPAEGYLVKSGNDLHSGIKSTRQNGVTISTFPGAEIKAAAPGTVSDVLWDDEY
                     GLTITIVHKYGFSTIYSHCQRATVKVDQKISKGEIIGYVGKTGNAMDHKLFYQIKIGM
                     DYIDPLPYLNKIYRPVPSSISN"
     assembly_gap    3122..3221
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(3256..4143)
                     /locus_tag="P5123_05675"
     CDS             complement(3256..4143)
                     /locus_tag="P5123_05675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017380504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HRX15781.1"
                     /translation="MRLIRNSNNYGYFFIMPFFILFFIFSLYPIIYTFILSLHEYDGL
                     SDKVFVGMDNYSRLIGDKYFWMSIKSTWRIWIVNIIPQITIALWLAAIFTFNRIKGQA
                     FFRAFFYLPNLVTAASIALLFNVLLDWQNGVINQALASAGLITEKINWLRIPSASQNW
                     VAFIQYWMWYGQTTILLLAGMSAIPTSYYEAAIADGANKIKIFFFITLPLLRPTMIYI
                     TVTSMIGGMQIFDIPMVLTDGAGAPEAALQTMVIYLYNQAFRFNNMGFGSAIAYGIFA
                     LIAVLSLIVYVLIQRKEDN"
     gene            complement(4729..6048)
                     /gene="xylA"
                     /locus_tag="P5123_05680"
     CDS             complement(4729..6048)
                     /gene="xylA"
                     /locus_tag="P5123_05680"
                     /EC_number="5.3.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015919215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0009045 - xylose isomerase activity
                     [Evidence IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA];
                     GO_process: GO:0042732 - D-xylose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="xylose isomerase"
                     /protein_id="HRX15782.1"
                     /translation="MSGLFPSIPKIEYKGRESKDPFSFKFYDPEEVIMGKKMKDHLRF
                     ALSYWHTLTGEGTDPFGDATAVRPWDSISDSLEQAKVRVKAAFEMMEKLGIGYFCFHD
                     RDIVPEADTLEEYQKNLDIIADEIEKEMKRTGIKLLWGTSNCFSHPRFMHGAATTCNP
                     EVFAYAAAQIKKAMEITHRLGGEGYVFWGGREGYETLLNTDMKLELDNFARMLKMAVD
                     YAKKIGFKGQFYIEPKPKEPTKHQYDVDTATVLGFLREHGLYDYFKMNIEANHATLAM
                     HTFHHELYLSRINGKLGSIDANQGDLMLGWDTDQFPSNIYDSTFAMYEVLKAGGFTTG
                     GLNFDAKVRRPSFEVEDLFLGYIVGMDTFAQGLRIAAKMVEDNVLDSILEKRYSGFKE
                     GIGKQIVDNKVGFEELSEYAKKNGVSKMESGRQEMIEAIINQYILCS"
     gene            complement(6216..7070)
                     /locus_tag="P5123_05685"
     CDS             complement(6216..7070)
                     /locus_tag="P5123_05685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010174595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HRX15783.1"
                     /translation="MQGMNIATTKGAKRFTLFYVYVILGVLTLLCAFPLYIMFINSTR
                     SNVEILNGISFLPSTYTVDNYFTMQRKVPIWHGFLNSAMITVPFTILAAYFSSLTAYG
                     FAMYNFRFKKYIFGFVLATMMVPMQLGLIGYFEFCNYFGLLDTYFPLIITGAVNTGSV
                     FFIKQYTEQSLPVSLLEAARMDEATEVQIFHRIALPIISPSIATMSIFNFVFCWNNYM
                     GPLVLLFSESKYPLPVLISLVRGTAHRTDFGAMYLTITLSVLPIIIAYAFFSKYLISG
                     LTVGAVKE"
     gene            complement(7082..7960)
                     /locus_tag="P5123_05690"
     CDS             complement(7082..7960)
                     /locus_tag="P5123_05690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006313796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HRX15784.1"
                     /translation="MRNSNNYGYFFILPFFILFFVFSLYPIIYTFILSLNEYDGMADK
                     VYVGFKNYKTLIGDHYFWMSIKSTWRIWLVNIIPQVGVALWLAAVFTFNRIKGQAFFR
                     AFFYLPNLVTAASIALLFNVLLDWQNGVINQALYGLGLVTEKINWLRMPSASQNWVAF
                     IQFWMWYGQTTILLLAGMSAIPTSYYEAAIADGANKTKIFFFITLPLLRPTMIYVIIT
                     SMIGGMQIFDIPMVLTDGRGAPEYSLNTMVIYLYNQAFTYNNMGFASAIAYGIFMLIA
                     VFSFIVYFMIQRKEDN"
     gene            complement(8074..9324)
                     /locus_tag="P5123_05695"
     CDS             complement(8074..9324)
                     /locus_tag="P5123_05695"
                     /inference="COORDINATES: protein motif:HMM:NF024808.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HRX15785.1"
                     /translation="MKKLWALVVALLLVAVAVGCGKKQQSTKLSIWAFTDELKKVVDD
                     FKAANEGIDVSFSIIPSEEFRTKIRPVLRSGSGAPDVFLGEVAWVRDIVSWGMWMDLT
                     KNYPDMVKQYEDQAPPYVVDLGRDENGHVVAMSWQACPGGFFYKRSVAKNVFGTDDPK
                     AIGELISTPEKFLDAARTLKSKNVKMLTGFSDYQWIPFYGRKAGWVDSNNNLFIDPVA
                     MDFFELAKTLRQEGLTAKVDQWSPGWFGNMAKKEVFGYLLPTWGLHYVIKKNAGDTAG
                     DWAVTRGPASYSWGGTWLGIYKNAEAEDLAVKFVNMLAVDADYQEAYTREVGDFMSNL
                     NVDAKMAKEEGDPFLGGQNHYQFFMEEAKNITPGLETKYDQDINGMYIDAIRNYIEDK
                     MTKEEAIADMKGKVKNAFPEINVD"
     gene            complement(9573..11210)
                     /locus_tag="P5123_05700"
     CDS             complement(9573..11210)
                     /locus_tag="P5123_05700"
                     /inference="COORDINATES: protein motif:HMM:NF015983.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4080 domain-containing protein"
                     /protein_id="HRX15786.1"
                     /translation="MKKILLVGLNARYTHSNLALYYIASQVKSSSNYKASIYETSINA
                     PYLSIMEQCLDEKPDVIGFSVYIWNSDLVKRLVDDINRIKPSLKIILGGPQITYNHRD
                     WREHEAVLIMGTETSFKAAMLDDFAKNEYSEPVDEFDIIGFPYNQAQIESLKKGYVYY
                     ESSRGCPFSCSFCLSSAQKMLSYRSLDKVFDELRFFIKNRVKTVKFIDRTFNSDSSRA
                     RKIWTFLINNINETLFHFEIEPSLLQEEDFEILEMVPDGLFQLEVGIQSVNLKTLKEV
                     KRAGSPERILNAVRRLSRLRNIHLHTDIIAGLPYETVESFERTFNRIYMAGADNIQLG
                     FLKVLPGTVMSHKKDEYSIICSKYPPYEIFSNKWMSFDEINFLRNVEKLIGGIHNCGK
                     MKNYVRYLGSRFLSPFEHYRKLVIFAKKTDFDLSTKDFDKIAEFLINYHNIVGDGDLV
                     CDILVYDRLFCSKTLSFPAFFSFKSGSIDKKKFITHIRNQFGEAFSKAIAKKSLPFRL
                     TQKSAAFLGFEKNTVAVFCNGEPCLPRKTGCYTIFFR"
     gene            11640..12167
                     /locus_tag="P5123_05705"
     CDS             11640..12167
                     /locus_tag="P5123_05705"
                     /EC_number="2.4.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012507281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003999 - adenine
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0006168 - adenine salvage [Evidence IEA];
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenine phosphoribosyltransferase"
                     /protein_id="HRX15787.1"
                     /translation="MEIKEAIRTISDFPKEGIQYRDITTLLQNGKAFKETIDIFHDRY
                     INRSIDIIAGIEARGFIIGSALAYALGTGFVPIRKQGKLPSEVYTKTYQLEYGSDTIE
                     MHKDSIKEGARVVLVDDLLATGGTILAAASLIRDAGGIIDELAFIVDLPDVGGHSKLT
                     ESGYPFYYICQFEGE"
     gene            12312..>12833
                     /locus_tag="P5123_05710"
     CDS             12312..>12833
                     /locus_tag="P5123_05710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012603504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glyceraldehyde 3-phosphate dehydrogenase
                     NAD-binding domain-containing protein"
                     /protein_id="HRX15788.1"
                     /translation="MSIKIGINGFGRIGRFVFRRAMEVDGVEVVGINDLIDVDYMAYM
                     LKYDSTHGKFKGTVEVKGKDLVVNGKTIRVTAERNPADLKWDAVSADVVVESTGLFLT
                     DESARAHIQAGAKKVIMSAPSKDATPMFVMGVNHNSYAGQDIVSNASCTTNCLAPIAK
                     VLNDKYGIKDGLMT"
BASE COUNT         4238 a         2573 c         2042 g         3880 t
ORIGIN      
        1 ccctctgcag tctgaacaaa agacatacca tcttcagtat ttctttctgc ctgacgaaga
       61 ccttgaatct gtgtacgcat cttttcagat acagccaaac cggaagcgtc atcacccgaa
      121 cggttaatac gcataccaga agccagtttc tcagttgact tgtttacatc ccaatgttta
      181 aattttaaca ctttattagc gttgatggca ctcatgttgt gattaactat cataatacac
      241 tcctttgttt acacccccaa tctccatatc ggggacaaaa attgccgcta tcagaatgtt
      301 gtaaaaagag ttttcaaaga acttcgataa gattccaatc tatgtaaaaa acacgatact
      361 ctccttaaga aaaaaggaga tgtggctcaa ctattgaagc agcaacaaca cactattact
      421 catttcatta gcctgagcca acatcgcagt accactctga acaagaacct gagtcttggt
      481 aaaattaacc attacctcgg ccatatccgc atcccttatc cgcgattcag atgcctgaat
      541 attctcgtat gcgttcaata aaccttttga cgcaatttca agacggttat aatatgcccc
      601 tagatccgcc ctctgtttgg aaatcttgtg aattgcctcg tcaagtacac ctatacttcg
      661 attcgaagat tcagctgttg acaatgtcat aagctctccg acttcacttt ttagaaacag
      721 tgcctgagca gtcattgtct gaataaatac tctttcacgc tgatgcatat tcgccccaat
      781 atgaaaccac atggacgcac gcgcattaac tctcgaaaaa tctcctaaca gcagattcat
      841 tttattgaat tccgcctgcg aagcaattcg atctacttca tcaataagtg ctgatacctc
      901 tacctgtatc aattggcgat cctccatggt gtaaatacca tttgaagact gaactgccaa
      961 cactctgatt ctttgtatta tttcatggag ttgctgcaaa taaccttctg cagtctgaac
     1021 gaaagacatg ccatcttctg cattacgctc tgcctgacgt aaaccctgga tttgggttct
     1081 catcttctca gagacagcca accctgaagc atcatcaccg gctcgattga tgcgcattcc
     1141 tgaacttagc tgttcagtat acttatcaag attccaatgt tgaaacttta atacacggtt
     1201 agcatttata gctgacatgt tatggttgat aatcaccaga ttcctccatg aatccgctat
     1261 cccagggaaa cagcatcctt gccgttttta aaaatccccg ttactatatt tgtatcgaat
     1321 atagtaactc atttctatac atcgattaca accatttata gctatttatg ggtatttata
     1381 agcaaataag gctgcttaag aagtccagat gacttgtttt gagatttgaa caaaaaagga
     1441 gctatttata ttttcgacac agttgatttt tttacatgaa tacttttctt gatttttttt
     1501 aaaaaaaaca atatatcggg tttatttgtg tttaaacgca cataacttca cttatttatt
     1561 aacttgacac aatttaccga tataatatag tgaattcata tttttaattg gagaggatcg
     1621 aacatgggat taaacccctt taaagatgcc gatctttatg atcttgaaac gtacctctgc
     1681 aaaagaatgg tcggtatttc tcgtaatgta aaatcggttt tatcatcttt tatctccggt
     1741 ataaaccgaa agggtaacga gaaaataacc cttatgttta ttcctcactc tgaaaagcga
     1801 attatcaact tccatatatc gatatactcc atagtgatat tctctttctt tttaatttcc
     1861 gttgtaacaa caaccaccat catgatcatg aatcatacca ctaccattaa agaaataaat
     1921 cgtttacgtc tttacggcta tgattctcaa atgcaaatta gtcactataa gaaagagatc
     1981 aacagcttgc atgatacttt tcagaaattt aagcccgaat taacgtatct ctattcgcta
     2041 acaccagata attatgtaga ttcactctgg gctaaaggtg gtgcaaccgg agatatagct
     2101 gaggctccag tttcagatga tgaacaaagc gatttttcgg ttactgtcga aaacagcaat
     2161 actgcccctg atattgaaga actcaacatt caggaaatgg gacaagagct aaaaacgacc
     2221 aaagaggtat taaagaaaat taaggttttt ctttcaaatc gtaaaaaaat aatcgaaaac
     2281 acaccttcaa tttggcctgc cgaaggatat cttgtgaagt ccggcaatga tttacacagc
     2341 ggaattaaat ccacacggca aaatggtgtt acaatatcaa ctttccctgg tgccgaaata
     2401 aaagcagccg ctccaggtac tgtaagtgac gtactttggg atgatgagta tggtcttacc
     2461 ataactattg ttcataagta tggtttttct actatttatt ctcattgtca acgagcaaca
     2521 gtaaaagtcg atcaaaagat atctaaaggt gaaataatcg gttatgttgg taaaaccggt
     2581 aacgccatgg accataagtt attttaccaa ataaaaatcg gaatggatta catcgaccct
     2641 ttaccgtatc ttaacaaaat atatcgtcct gtaccctctt caatttctaa ttaaataata
     2701 actacctgaa catcggtggt tattatttta aatcaattct aaatttttat agcattttta
     2761 tttatcaaat tgtccctttg ataatatata aatttttttt gctctcttcc gtcttttatc
     2821 tgctttccga actcattcct gtcgacctgt tactattttg ctatatcgat ttcaaaccag
     2881 ataacaactt tggtcaatat atcttttcgt agtatagctg catattcacg cagaatggtt
     2941 attttaccgg attatctcca atcaaaaaga tttctgcttt aataacttta aatttcttta
     3001 catcattaat aaagaaaagg cgcctattat ttatggcgcc ttttctgcaa aaatagctgt
     3061 taaatcatta ctctttaacg gcaccaacag ttaaaccgct tatcagatac tttgaaaaga
     3121 annnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     3181 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn ngtagcaata ttcattccct
     3241 gcattataat tccccctagt tatcttcttt acgctgaatc aaaacataaa caataagcga
     3301 aagcactgct attaatgcaa aaataccata agctatagct gatccaaacc ccatgttgtt
     3361 aaacctaaac gcttgattat acaaatatat aaccattgtt tgcaaagctg cctcaggagc
     3421 accggcacca tcagttaata ccatcggtat atcaaaaatc tgcataccac caatcatact
     3481 tgttacagta atataaatca ttgtgggtct taacagtggc aatgttataa aaaagaatat
     3541 ttttatcttg tttgcgccat cagcaatagc tgcctcgtaa tacgatgttg gtatcgccga
     3601 cattcctgca agtaacagaa tagtcgtctg tccataccac atccagtatt gtatgaaagc
     3661 tacccagttt tgtgaagcac ttgggattct tagccagttt attttttctg taattaaacc
     3721 cgctgacgct aatgcttgat taataacacc gttttgccaa tcaagaagga cattaaacag
     3781 cagagcaatc gaggcagcag ttacaagatt tggcaaatag aaaaaagctc tgaaaaaagc
     3841 ctgcccctta atgcgattaa aagtaaaaat agccgcaagc cataaagcaa tcgtaatctg
     3901 cggaattata tttactatcc agattctcca agtagattta attgacatcc agaaatattt
     3961 atcacctata agtctggaat agttatccat accgacaaaa actttatcag acaaaccatc
     4021 atactcatgc aaacttagta taaaagtata gatgattgga tacagtgaaa aaataaaaaa
     4081 taaaatgaaa aaaggcatga tgaaaaaata accgtaattg ttggaatttc gtattaatcg
     4141 cattttagcc ctcttaaccc ttataaaaat tgtttaataa tactttggaa agaaattata
     4201 ataagtttat actgtaaata ttacaatttc aatttactat atattcgcaa ccctttgcat
     4261 taacaagcaa aaaatatttc gtgaattctt tttatgtata aacaatactt cttcacaatt
     4321 atgctactca tgcaggtaaa ctataaatat tgattacctt gctttattgt aaactgaaca
     4381 tagcattact actccttaac agctcctaca gttaaaccat ttatcaaatg ttttgaaaac
     4441 aaaatataag cactaattat tggcaataca gataatgtga ttatcagtgt tgatgctcca
     4501 aaatttgtac gatatcaggt acctgtcaca aaagccaata aagcggttaa tggatattta
     4561 ttttcagaga ataaaaggat cagaggagcc atatagatat tccagcaaga tacaaaatta
     4621 aaaatagcta cggattattt tataatcata cataacttct aaactaaaca aacaattata
     4681 aaaaaacaca gccataagct gtgttttttt ataattgttt gttaaaattt acgagcaaag
     4741 aatatattgg ttgataattg cttcaatcat ctcctgacgg cctgactcca ttttagagac
     4801 accatttttc ttggcatatt cggataactc ttcaaaacca actttattat caacaatttg
     4861 ctttccaatt ccttctttga acccactgta acgtttttca agaatagaat ccagaacatt
     4921 atcttctacc atttttgctg caattcttaa tccctgtgcg aaagtatcca tacctacgat
     4981 atacccaagg aaaagatctt caacctcaaa tgatggacgt cgaacctttg catcaaagtt
     5041 caatccgcct gttgtaaatc cacctgcctt taatacttca tacattgcaa aagtagaatc
     5101 atatatgttt gaagggaact gatccgtatc ccatccaagc ataaggtcgc cctgattagc
     5161 atctatagat ccaagctttc catttatacg cgataaatac aattcatggt gaaaagtatg
     5221 cattgcaaga gtagcgtgat ttgcctcaat attcattttg aaataatcat acagcccgtg
     5281 ttctcttaag aaacccaata ctgtcgctgt atcaacgtca tactgatgtt tagttggctc
     5341 ctttggttta ggttcaatat aaaattgacc tttaaaacca attttctttg cgtaatcaac
     5401 agccattttt aacattctcg caaagttatc caactctagt ttcatatcag tattaagaag
     5461 tgtttcgtaa ccttcacgtc caccccagaa aacatatccc tcaccgccaa gcctatgagt
     5521 tatttccatt gcttttttaa tttgcgctgc cgcatatgca aaaacttccg gattacacgt
     5581 agttgctgcg ccatgcataa atcgcggatg agagaagcag tttgaagtac cccataaaag
     5641 tttaataccg gtacgtttca tctctttttc aatttcatcg gcaataatat ccagattttt
     5701 ttgatactct tcaagagtat cagcttcagg cactatatct cgatcatgaa aacagaaata
     5761 acctatacca agtttttcca tcatttcgaa agcagcctta actctcactt ttgcctgttc
     5821 aagagagtca cttatagaat cccatgggcg tactgctgtt gcgtccccaa atggatctgt
     5881 accctctcct gtaagcgtat gccaatatga caaagcaaaa cgcaaatgat ctttcatctt
     5941 tttacccatt attacttctt cagggtcata aaatttaaaa gagaaaggat ctttagactc
     6001 gcgacctttg tattcaattt ttggaattga cgggaataat ccactcatct ccatacctcc
     6061 gatatttttt tgattaatca ctttttaaag tcattgtaaa aaccacttaa atatattatt
     6121 gtgaagaata caataatttt ttaaaaaaag agtgcaataa gcactctttt tttaaaatgt
     6181 tgaaaattac ttaaatttgc aaacctatct aaatattact ctttaacggc accaacagtt
     6241 aaaccgctta tcagatactt tgaaaagaag gcgtatgcaa ttataatcgg taaaacagat
     6301 aaggtaattg taagatacat cgctccaaag tcagttctat gagccgtacc tcttacaagt
     6361 gaaatcaaaa caggaagtgg gtatttgctc tcagagaaga gaagaacaag tggtcccata
     6421 tagttgttcc aacaaaatac gaagttaaat atggacatcg tcgcaattga tggagatata
     6481 atcggtaaag caatcctgtg aaagatttga acctctgttg cttcatccat tcgagctgcc
     6541 tcaagcaacg aaacaggtaa cgattgctct gtatactgct taatgaaaaa tacagatccc
     6601 gtattaaccg caccagtaat aatcaacgga aaataggtgt ccagtagtcc aaaatagtta
     6661 caaaactcaa aataaccgat cagaccaagc tgcattggca ccatcattgt agcgagtaca
     6721 aaaccaaaga tgtatttctt aaaacgaaaa ttgtacatcg caaaaccata agccgtaagt
     6781 gaagaaaaat aagctgccaa aattgtaaat ggtacagtaa tcatggcact gtttaaaaaa
     6841 ccatgccata ttggcacctt acgttgcatt gtgaaatagt tatctacagt ataagtacta
     6901 ggtaaaaaac taattccatt caatatttca acattgcttc tggttgaatt aataaacatt
     6961 atgtatagag gaaaagcgca tagcagtgtt aaaactccaa gaataacata cacataaaaa
     7021 agtgtaaaac gctttgcccc ttttgttgta gcaatattca ttccctgcat tataattccc
     7081 cctagttatc ttctttacgc tgtatcataa agtaaacaat aaacgaaaat accgcgatca
     7141 gcataaaaat tccataggcg attgcagagg caaaccccat attgttataa gtgaaagcct
     7201 gattatatag atatattacc attgtattca atgaatattc gggagcacca cgaccatcag
     7261 tcaataccat cggtatatca aaaatctgca taccaccaat catactggta ataattacat
     7321 aaatcattgt tggacgcaat agtggcaaag tgataaaaaa gaatatttta gttttgtttg
     7381 ccccatccgc aatagcagcc tcataataag atgttggtat cgctgacatt cccgcaagta
     7441 acagaatagt cgtctgtcca taccacatcc agaactgaat aaaagctacc cagttttgtg
     7501 aggcgcttgg catacgaagc cagtttattt tttcagttac taaacctaga ccataaagag
     7561 cctggttaat aacaccattt tgccaatcaa gaaggacatt aaagagcaat gcaatagaag
     7621 ctgctgttac taggtttggt aggtagaaaa aagccctgaa aaaagcctgt ccctttattc
     7681 tgttaaaagt aaaaaccgcg gcaagccata aagctacacc gacctgtgga ataatattca
     7741 caagccatat tcgccatgtt gatttaattg acatccagaa atagtggtca ccgattaatg
     7801 ttttgtagtt tttaaagcct acataaacct tatcagccat accgtcatac tcattaagac
     7861 ttaaaataaa agtatatata ataggataca gagaaaacac aaaaaatagt ataaaaaacg
     7921 gcaagataaa aaaatagccg taattattgg aattacgaat tagtctcatt tttctcctcg
     7981 aggaatacat tttctttgtt tataaaacca tcagccggga gagtaaaata aaggggccga
     8041 agccccttta ttcgtagaat tcaataaaag ctattagtca acattgattt cagggaaagc
     8101 atttttaact ttacctttca tgtctgctat tgcttcttct ttagtcattt tgtcttcgat
     8161 gtagttacgg atagcatcga tgtacatacc attgatatcc tggtcgtatt tagtttcaag
     8221 acctggagta atgtttttag cttcttccat gaagaactgg tagtggtttt gtccaccaag
     8281 gaaaggatct ccctcttctt tagccatctt agcgtcaacg ttaagatttg acataaagtc
     8341 accaacttca cgagtataag cttcttgata atctgcatca acagcaagca tattaacaaa
     8401 tttaacagcc aagtcctcag cttcagcatt tttgtaaata cctaaccaag taccacccca
     8461 gctgtaagaa gcaggaccgc gagttactgc ccagtcacca gctgtatcgc cagcgttttt
     8521 cttgattaca tagtgaagac cccaagtagg aagaagataa ccaaatactt ctttcttagc
     8581 catatttcca aaccagcctg gagaccactg atcaacttta gctgtaagcc cttcttgacg
     8641 aagagtttta gcaagttcga agaaatccat agcaactgga tcgataaaaa gattattgtt
     8701 gctatctacc caaccagcct tacgtccgta aaaaggaatc cactggtagt cagaaaaacc
     8761 agtaagcatt tttacgtttt tagacttaag agtacgtgca gcatcaagga acttctcagg
     8821 tgtgctaata agttcgccga tagctttagg atcatcagtt ccaaaaacat tcttagctac
     8881 agaacgctta tagaagaaac cacccggaca tgcttgccaa gacattgcaa caacatgacc
     8941 attttcgtca cgaccaaggt caacaacgta tggtggagct tgatcttcgt actgttttac
     9001 catgtcagga taattcttgg taagatccat ccacattccc cagctaacga tgtcacgtac
     9061 ccacgcaacc tcaccaagga atacatctgg cgcaccagaa cccgaacgaa gaactggtct
     9121 aatttttgtt ctgaattctt cactaggaat aattgagaac gaaacgtcga taccttcgtt
     9181 tgcagcttta aagtcatcaa ctaccttttt caactcgtca gtaaatgccc aaattgatag
     9241 ttttgtgctt tgttgttttt taccacaacc tactgcaaca gcaacaagaa gcagtgcaac
     9301 aacaagtgcc caaagctttt tcataaaaac ctcctattta aacaaacgct tttttaagac
     9361 tttttataat acgaccttgc atgaagttaa aagcacgcga gttttaagtc aatccctttt
     9421 tttaaatttt tttaacagca tagagattaa ttgtaacata tagtaaaaaa cccaaccgga
     9481 tttttgtatc aatacggtta ttattatatt tattaattaa atatttggca atatctaata
     9541 attaactttt aaataaggtt gcatctgtta cattatctaa aaaaaatcgt gtaacacccg
     9601 gtttttcgag gcagacacgg ctcaccgtta caaaaaacag ctactgtatt tttttcaaaa
     9661 cccaggaaag cagcactttt ttgcgttaac cgaaaaggca gacttttttt agcaatcgct
     9721 tttgaaaaag cttcgccaaa ctggttgcgg atatgggtta taaacttttt tttatcaata
     9781 cttccgcttt taaaagaaaa aaaagcaggg aaactcaatg ttttactgca aaacagccta
     9841 tcatatacaa gaatatcgca tacaagatca ccgtccccaa ctatattatg gtaattaatt
     9901 aaaaattcag caattttatc aaaatcctta gttgaaagat caaaatcagt ttttttagca
     9961 aatattacaa gtttacggta atgttcaaat ggagataaaa agcggcttcc caagtatcta
    10021 acataatttt tcatttttcc acaattatgg ataccgccta tcagtttttc aacatttcgc
    10081 aagaaattta tttcatcaaa actcatccat ttatttgaaa aaatttcgta tggcggatat
    10141 ttagaacaaa taatgctgta ttcatctttt ttatgagaca taactgttcc cggcaacacc
    10201 ttaaggaaac cgagctgaat attatcggca cctgccatat aaattcggtt aaaagtacgc
    10261 tcgaaggact caactgtttc ataaggtaaa ccagcaataa tatcagtatg cagatgtatg
    10321 ttgcgaagtc gagaaagacg ccttacagca tttaaaatcc tttctgggct acctgcacgt
    10381 ttaacttctt ttaaagtctt taaatttacc gattggatcc cgacttctaa ttggaaaaga
    10441 ccgtcaggaa ccatttcaag tatttcaaaa tcttcttcct gtaaaagaga gggttcaatt
    10501 tcaaaatgga aaagcgtctc atttatatta tttattaaga aagtccatat ttttctcgct
    10561 cgagaagagt cactattaaa ggttcgatca ataaacttta ccgttttaac gcgattcttg
    10621 ataaaaaacc gcagttcatc aaagacctta tcaagtgaac ggtatgaaag catcttctgt
    10681 gcagaggaga gacaaaaaga gcaactaaaa ggacatccgc ggctcgactc ataatacaca
    10741 tagccttttt tcaaggattc aatctgtgct tgattgtatg ggaagccaat aatatcaaat
    10801 tcatcaaccg gctccgaata ctcattttta gcaaaatcat caagcattgc agctttaaag
    10861 gatgtctccg ttcccattat caaaacagcc tcatgttccc tccaatctcg atgattatag
    10921 gtaatctgag gcccaccaag aattattttc agagatggtt taatgcgatt aatatcgtca
    10981 acaagccttt tgaccagatc actattccag atataaaccg aaaaccctat aacatcaggc
    11041 ttctcatcaa ggcactgctc catgatagaa agatacggag cgtttatact tgtttcataa
    11101 attgaagcct tataattact gctgcttttc acctgagacg caatgtagta gagcgccaga
    11161 tttgaatgtg tatatctggc atttagccca acaagaagaa tttttttcat tgtccagtcc
    11221 tgaccttatt attaatcttt caattttagt ttcataattt tttcgaagtt ccatgttgac
    11281 aattaagtta ccaagtttta tctgtataca agaaagaaac attgcaacac taaggaactg
    11341 ctatgaacca ctatatccta gaaattatca tcagctaaat ggttgttcca aaagagtttt
    11401 tcacaagctc ctttgctgtc gcccagcagc actttgaaaa cgcaaagagg ttccggactg
    11461 ctttcacatg cgaccttgtg gaaaaaattt tttaaagaga ttaccacttt ttaatagaga
    11521 cccatataaa cgacaataac actttttatg caaactgtta tcagtataca ccttttttta
    11581 agttaggaag atgaaatctt aaacaacata tctaaaaaaa aagattctcg gagaatataa
    11641 tggaaataaa agaggccata agaaccatat cggactttcc caaagagggc atccagtaca
    11701 gggacataac taccctgcta caaaacggta aagcgttcaa agaaacaatc gatatttttc
    11761 acgaccgata cataaaccga tccatcgaca ttattgccgg catagaagct aggggtttca
    11821 taatcggatc agctctcgca tacgccttgg gtactggttt tgtaccaata cgcaaacagg
    11881 gcaaactccc atcggaagtt tacaccaaaa cttatcaact tgaatatggg tcagatacta
    11941 tagaaatgca taaagattct ataaaagagg gagcccgggt tgtactcgtt gatgacctgc
    12001 ttgcaaccgg aggcaccatt ttggcagccg cctctctaat acgcgacgca gggggaataa
    12061 tcgacgaact tgctttcatt gttgacctcc ccgatgttgg cggacacagt aaacttacag
    12121 aatctggtta tccgttttat tatatctgcc agtttgaagg tgagtagttt tcctgcttgt
    12181 gaataatctc acatttagta ataaattctt gacactcaaa ccactgctta tgtagttgaa
    12241 ctgtatttgt taggccttaa aacagaatgc attttgtgtt ctaatactaa atataacgag
    12301 gtaaatctat tatgagtatc aaaataggta ttaatggttt tggtcgtatc ggacgttttg
    12361 ttttccgaag agctatggaa gtagatggtg ttgaagtagt aggaattaac gatcttatcg
    12421 acgttgatta catggcttat atgcttaaat acgactccac acacggcaaa ttcaaaggaa
    12481 cagtagaagt taaaggcaaa gatcttgttg ttaacggaaa aacaatccgt gtaaccgctg
    12541 aaagaaaccc tgctgacctt aaatgggacg ctgttagcgc cgacgttgtt gttgaatcta
    12601 ctggtctttt tcttacagac gagtcagcac gagctcacat acaagccggt gctaaaaaag
    12661 taatcatgtc tgctccttcc aaggatgcaa ctccaatgtt cgtaatgggt gttaaccata
    12721 actcatatgc tggacaagac atagtttcca acgcttcttg tacaacaaac tgtcttgctc
    12781 ctatagcaaa agttcttaat gacaaatatg gaatcaaaga cggacttatg aca
//
LOCUS       DAOTGZ010000061        12738 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_3064
            NODE_5887_length_12738_cov_5.513759, whole genome shotgun sequence.
ACCESSION   DAOTGZ010000061 DAOTGZ010000000
VERSION     DAOTGZ010000061.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868088
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 12738)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 12738)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:22:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,785
            CDSs (total)                      :: 2,751
            Genes (coding)                    :: 2,732
            CDSs (with protein)               :: 2,732
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 12 of 19
            Pseudo Genes (internal stop)      :: 5 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12738
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5887_length_12738_cov_5.513759"
                     /isolate="AMR_MDS_3064"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..597)
                     /locus_tag="P5123_05715"
     CDS             complement(<1..597)
                     /locus_tag="P5123_05715"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRX15789.1"
                     /translation="MKIRVIFILTVMLCCLTIMSGCDADDESNYKVDKEKAEAFAGPI
                     LEKLKSETPVYAEDFSDNSKGWYINTEESYGYTANVSTEGKYDLDIDTKQLGADFPDS
                     PILSDFVLQFDFVYKSGGNDSSCYFIYRNQEVLEPGNSNSYGLEIYYNSLLRLYKYIQ
                     EYGGDATIRDINMDKFDLLEVNTYTIIVKGTTHIVYVND"
     gene            866..1486
                     /locus_tag="P5123_05720"
     CDS             866..1486
                     /locus_tag="P5123_05720"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRX15790.1"
                     /translation="MYTENSTKRYTESIVKIIETIKQSSLKDYVNRALDLYSNIDEKA
                     SIADIESRLPKFESDNIEDYITTSVSANQIQALVFHWNYDGGSSDCIAFGSLYESCTY
                     DESGDTDKYNFEMNITTNNCIAEEYADMKVNVMKPWYDLDMEGDIEDDDLSAEINTLF
                     TAKFCLCFQKACQNVYNGLDKAFIKHPFHIFIERHGRWPMHVYSIE"
     gene            1589..1843
                     /locus_tag="P5123_05725"
     CDS             1589..1843
                     /locus_tag="P5123_05725"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRX15791.1"
                     /translation="MLIDSNDIIIGKYYPDIAKAIPLGHVSIILFFVIFVLFAINYFR
                     PRYCPYCNSRMITEKNNSSNTTIFICQGGSYKIDTHMNRY"
     gene            complement(1942..2958)
                     /locus_tag="P5123_05730"
     CDS             complement(1942..2958)
                     /locus_tag="P5123_05730"
                     /inference="COORDINATES: protein motif:HMM:TIGR04085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SPASM domain-containing protein"
                     /protein_id="HRX15792.1"
                     /translation="MVSVKKTLSKVFHKLSGFSIEHCGLLVIELTSDCNCACPVCAPA
                     HVRNQIKRGYMSREIFDKIVVEAGDLNPRQVCLYARGESLLHKGAVDMTRVLSDAGFY
                     TELVTNGELINPEISKDLYDAGLSKLVISYPGISSKNYRMARGKNEPQGLADNIVKSI
                     EQWSCGDREVSLRSLIFHKTKSEIDDWVINLVDDRLPDFLDKWLSVEGLSSVEIHGYM
                     PWPRFYDETYLSHLTTYRRRCEHALNAMVVYWNGDVTPCSYDVHSELCIGSINNETLS
                     SLYNNSKMRQFRKRMYSHKISKLDDVCRNCLIPRYPVPLKLYTPDRKEESIREIREII
                     AQRG"
     gene            complement(2983..3474)
                     /locus_tag="P5123_05735"
     CDS             complement(2983..3474)
                     /locus_tag="P5123_05735"
                     /EC_number="1.5.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010243389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016646 - oxidoreductase activity, acting
                     on the CH-NH group of donors, NAD or NADP as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavin reductase family protein"
                     /protein_id="HRX15793.1"
                     /translation="MSKKEAVFKSCLQPASKVLVSCRGLDGENNALAVGYCCNCSYDP
                     PMVMVGIVPSRHSYKMIKESGCFVVNLVDEAYKENFDYLGSVSKRDEDKLLKKSIKLS
                     EGIKVNAPILADCPVNIECTIVDSIVTGSHEMFVGKVEHVHADSNLVDGEGKIDFGQI
                     KFL"
     gene            complement(3508..4212)
                     /locus_tag="P5123_05740"
     CDS             complement(3508..4212)
                     /locus_tag="P5123_05740"
                     /inference="COORDINATES: protein motif:HMM:NF024811.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IA family hydrolase"
                     /protein_id="HRX15794.1"
                     /translation="MRYRFILFDVDNTLLDFDRSESNAFINTMKEYEVNCDEDLITKF
                     KEICAKEWDRLNLGKSDDIIIQKNYHKLYYKYSINRFERIKNTGISISAADMSELYLQ
                     KLSTEINYMPCALDICEALSKDIKLVIATNGIVDVQKKRLNDMNKYFFKQYISEEIGC
                     IKPSTVFFKTITEDLKVKPDDCLMVGDSLVSDIGGANRVSMDSCWYNPKRKFNNSKEI
                     PTYEISSLLDIMQIVK"
     gene            4382..5044
                     /locus_tag="P5123_05745"
     CDS             4382..5044
                     /locus_tag="P5123_05745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017039666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Bax inhibitor-1 family protein"
                     /protein_id="HRX15795.1"
                     /translation="MENVFDRRTVEQGQAIISERFYNFIIGLTLCWGFGINYLMVKHV
                     SPGFALQYGFWPFIIAYFIMCFLGIYLFTKYDEPLISFLGYNLVVVPFGFVINIVVSQ
                     YSPSLVLQAVQMTGIVTLIMMFLGTLFPAFFKKIVGALTIALFAMIIVELVAVYFFKT
                     HHTIIDWIVAAIFCGYIGYDWGRAQQIPKTVDNAVDSAAALYMDIINLFLRILRIMGR
                     RR"
     gene            5179..5427
                     /locus_tag="P5123_05750"
     CDS             5179..5427
                     /locus_tag="P5123_05750"
                     /inference="COORDINATES: protein motif:HMM:NF018028.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RDD family protein"
                     /protein_id="HRX15796.1"
                     /translation="MEFLPLKIDGVKTYAGFWKRFLSGLIDAIIFIPAIFIFNYFIKT
                     SIPVAILITVIQLSLYTIYSIYFNYYYGATIGKSNKII"
     gene            complement(5435..6073)
                     /locus_tag="P5123_05755"
     CDS             complement(5435..6073)
                     /locus_tag="P5123_05755"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRX15797.1"
                     /translation="MFSCSERYFYQKHLKDNYGDYHSKISYLSDKIAINQNDAEAYLE
                     RGRLYIGIGHYSKGIEDLDNAIIIKPDFGEAYLLRGYSKYNYFNYKKEMLDQAYDDAN
                     KMIELKYKICKAYLLRGRVQSFRNNYSDAIKDMDKAIEADINCGWAYYVRGQIKHDQY
                     DDYNGSLSDLKKAKTIFNEEGDKLGYDLTKDLIKFIKQRRLEDKLFKFLFGI"
     gene            complement(6159..7304)
                     /locus_tag="P5123_05760"
     CDS             complement(6159..7304)
                     /locus_tag="P5123_05760"
                     /inference="COORDINATES: protein motif:HMM:NF012245.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein"
                     /protein_id="HRX15798.1"
                     /translation="MGILGFGIVLSLSLGMALIRTIRDKIELIVSSVNRMGTGDFSEN
                     RKVTPVIDELSGANSTLYDMKEQVAGMFSDIIKASDVLTRSTDDISTITETFSSDTQN
                     QAATVEEVSATIEEVSASMDTISSGTTDQIKSLTVLTERIQELAQIINESEVKASESK
                     KMSQLISTYARSGEASLSRMDSNMQYISKSSEQMRAIISMINDISEQINLLSLNASIE
                     AARAGSAGMGFAVVADEVSKLAESTATSVKEIDTLIKKSDSEISEGKQYVLEVINEMK
                     KIIDGVANIAEMAGMISGYTIRQVESYKMVDDELRKVKEKSENIEIATAEQKRAMAEI
                     VESTSRINELTQSISSGAEEIAASSGDIAKMAASQKEMTTRFKIREV"
     gene            complement(7483..8025)
                     /locus_tag="P5123_05765"
     CDS             complement(7483..8025)
                     /locus_tag="P5123_05765"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRX15799.1"
                     /translation="MNIHKEIAASTVDSHSSLLRYFSLKFFLYTEFISYFIIVPILFI
                     NIILSLELDFEQLKMMFYITLVVVAISFSSTLINNHIVLAPVTKYFKALINKQNISEE
                     MYLEAQHRFFNLPLYHSIGSFFRWMIGLSLQFAPFLSLYNFTSIQKTNTWILVAIMAP
                     FGSIFYFLFFDDRDSYTKSS"
     gene            8649..9653
                     /locus_tag="P5123_05770"
     CDS             8649..9653
                     /locus_tag="P5123_05770"
                     /inference="COORDINATES: protein motif:HMM:NF024676.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="WYL domain-containing protein"
                     /protein_id="HRX15800.1"
                     /translation="MNHDILPSADTEIERFYQYLHILALLQNPNEIELKQKAEIYSFL
                     KIDFEQRYPHYDDQHKKTAKFITPKNIENYIKNTILNNEHLNIRSDKKRYVLNTPYRK
                     ESLLPFLRSYTAITVTDAGKQDVLLKIIKSHKNRFMWNLATVHFAIIERRILSIDYLK
                     SANADTKTSEIVPHHLVYRNNNLYLIGRVTGKDNDAQFIYTHIKKIKPTDKTTDLPIT
                     PLEDIYKDTIGSFFNMGKEPHNVKIRYCSDIKYDMERLLKNIEADITDISNFDSLYDY
                     EARFKLAEDTFFCKQIFGFGNKALIIEPADLKEMMINTAKKITNQYVTAHKSNQRDHK
                     "
     gene            9650..10717
                     /locus_tag="P5123_05775"
     CDS             9650..10717
                     /locus_tag="P5123_05775"
                     /inference="COORDINATES: protein motif:HMM:NF024676.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="WYL domain-containing protein"
                     /protein_id="HRX15801.1"
                     /translation="MINNSKIKDKVSSDANKDTRELLSKPNVPNDPATKIRIMERYLH
                     ILALIQNPEGIDGGWTDKKIADRINEVLENKEGSDDSLTDDKVNRDIKTLRDDLNISI
                     SGQGTSRRMIMDTLDPHLQITLLEAYAPFVITDNSRDLILDNIVQNNSDYCAWNIAAV
                     KYALTMNRYCNNISATYTLNFDYASKACNHTKDENREIVPYHLVFKNNNLYLIGLMLS
                     NGLPVHNSPTVYLFEKIKEIKLKKSDKITHTVPPVKEIYKNSTSSFINQKTHDVVIRY
                     TQSAQTELKRVLPETAIETEIKDANNSKYSYETRFISCDDIYLCKELFVFGDQIEIIK
                     PTELRKMMFDNAQAVLDAYGV"
     gene            complement(10734..11615)
                     /locus_tag="P5123_05780"
     CDS             complement(10734..11615)
                     /locus_tag="P5123_05780"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRX15802.1"
                     /translation="MKKTTLLFAIFILTAVTGCGQKSTQQNRNMEPLKEENIGVVNRY
                     HPVLIAWESREYVFSAGYIVGGSRDKLWYSLDDFDIHFMGKKMDADKYKDALHLNPVK
                     NETDLGQIDLAKGDETFVFYAFNKRIGSSKIKNPGIYTQWSTSDLMFGTFFKPISCAR
                     SFLIGVNGTWNALPRAVKPINKGNNYLVDLDGDEEIEKIGFERCKDKGGCNAARVTFV
                     FHGKTLHSDGYSVPADYDYYQNCVAEWLEIFPIDLNGDGKMELVIKAVYGYGVSVDVY
                     EVRPNRMVQIMTFYDGD"
     gene            11801..12313
                     /locus_tag="P5123_05785"
     CDS             11801..12313
                     /locus_tag="P5123_05785"
                     /EC_number="3.5.4.33"
                     /inference="COORDINATES: protein motif:HMM:NF025793.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0052717 - tRNA-specific adenosine-34
                     deaminase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside deaminase"
                     /protein_id="HRX15803.1"
                     /translation="MISDKMISAVLENQQQAIKSGNPPFAAIITVDDQIIATSYNQSR
                     SSGNPLKHAEISAIESAIKAHGASILQKAHLISSNEPCPMCVGACIWSGIPKVTYFIS
                     QEQVAAIRGWGKFMPAHDIAAADDSGIVINGPIDNDEMIKFHQDFWTADNNSQYKHSI
                     HLSGSVKTET"
     gene            12404..>12738
                     /locus_tag="P5123_05790"
     CDS             12404..>12738
                     /locus_tag="P5123_05790"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRX15804.1"
                     /translation="MKEYVFPYFGKLQMDHLEEYYDVNIDYGDSEIQIELTFENKTIE
                     TYIMDKTKNLIENISKFDKQNQIYIFNDYNNSDGDTVKTYLQHHLEAADKEELSRIIN
                     FEDTKIKQE"
BASE COUNT         4237 a         2562 c         2009 g         3930 t
ORIGIN      
        1 atcgttgacg tatacaatat gggtagtacc tttaacaata atcgtatagg tattcacttc
       61 taataaatca aacttatcca tattgatatc acgtatagtt gcatccccgc catattcctg
      121 aatatattta tataggcgca acaaagaatt ataatagatt tccaaaccat aactattact
      181 atttccaggt tctaatactt cctgatttct ataaatgaaa taacagctgg aatcattccc
      241 accgctttta taaacaaaat caaactgcaa tacaaaatct gataaaatcg ggctatccgg
      301 aaaatcagcc cctaattgtt ttgtatcaat atccagatca tattttcctt cagtcgagac
      361 attcgcagta taaccataag attcttcagt attaatatac cagcctttac tattgtcaga
      421 aaaatcttca gcatacaccg gagtttctga ttttaatttt tcaagtatcg gccctgcaaa
      481 ggcctcagcc ttttctttgt cgaccttata attcgactca tcatctgcat cacacccact
      541 cataatcgta agacagcaaa gcataactgt cagaataaaa ataactctaa ttttcattgg
      601 attacccccc aattttttat tctagttatc tttctttacc atattttgta tttttgatca
      661 agctattttc aaattatggg ttactggatt cattagcttt ttaaacttaa agttcttcag
      721 attgtaactc tactccaact gtattgttat ttacaatcct taataccata atcggcaata
      781 atgttgccaa acaaaaacaa cttgactgca cctccaaatt attaaaaaac ttcattctta
      841 ccaataatct gaaacggaga actgtatgta cacagaaaat tctaccaaac gctacacaga
      901 atcaatcgta aaaattattg aaacaatcaa gcaaagcagc cttaaagatt atgtaaacag
      961 agctcttgat ctgtacagca atatagatga gaaagcatcc attgctgaca ttgaatcccg
     1021 gctgcctaag tttgaatcag acaacataga ggactacata actacatctg tttccgctaa
     1081 ccagatccag gctcttgtct ttcactggaa ctacgacggc ggcagctccg actgtattgc
     1141 atttggttct ttgtacgaaa gctgcacgta tgacgaatcc ggagacacag ataaatataa
     1201 ctttgaaatg aatatcacta caaataattg tattgcagaa gagtacgcgg atatgaaagt
     1261 aaacgttatg aaaccatggt acgaccttga tatggagggt gatattgaag atgatgacct
     1321 gagtgccgaa atcaataccc tttttacagc aaaattttgt ctctgtttcc aaaaggcctg
     1381 tcaaaatgtt tacaacggtt tggataaagc atttataaag cacccgtttc atatttttat
     1441 agaacgacat ggcagatggc caatgcatgt atacagtatt gagtagtgtc gcataagaac
     1501 tggtcagcgt gtcccatgca accagctatg gttataaata atattttata atattattta
     1561 taacccttaa tacagggagc aatcaaatat gttaatagat tctaacgata ttattattgg
     1621 caaatactat cctgatattg ctaaagcaat tcctcttgga catgtatcaa taatactttt
     1681 ttttgttata tttgtactat ttgccataaa ttattttcgc ccaagatatt gcccgtattg
     1741 caactcccga atgatcacgg aaaagaacaa ttcatccaat accacaattt tcatttgcca
     1801 ggggggttca tacaagatcg acactcatat gaacagatac tgatacctgt tttcagaatc
     1861 aatagcaact gctgcatatt actaatgatc tgctgcaatc agaatatcac cgataatccc
     1921 atgcgcgatc ctttgcgaag attatcctct ctgcgcgata atctccctta tttcacgaat
     1981 agactcctct ttcctgtcgg gtgtgtacag tttcagcggc actggatacc tcggaataag
     2041 gcaatttctg cagacatcat ccaattttga aattttgtga gagtacatcc tttttctaaa
     2101 ttgacgcatc ttactgttat tataaagaga ggagagcgtc tcgttattaa ttgaaccaat
     2161 acacaattca gagtgcacat catacgagca gggagttaca tctccattcc aatagaccac
     2221 catcgcgttc agggcatgtt cacatcttct tcgatatgtt gtcagatgcg acagataagt
     2281 ttcgtcgtaa aaccgcggcc agggcatata tccgtgaatt tcgactgagg acaatccttc
     2341 cacagataac cacttatcaa gaaaatcagg aagcctgtca tcaacaagat taattaccca
     2401 atcatcaatt tccgatttag tcttgtgaaa aataagactt ctcaaggaaa cctcgcgatc
     2461 accacaactc cactgttcaa tggatttaac gatgttatct gccagcccct gcggttcatt
     2521 ttttcctcga gccatacgat agtttttact gcttattccc ggatacgaaa tcacaagctt
     2581 acttagcccg gcatcataaa gatctttaga tatttccggg ttaatcagct caccgtttgt
     2641 aaccagttca gtataaaacc cagcgtcaga aagaacgcgg gtcatgtcca cagcgccttt
     2701 atgtaataac gactcgcccc gggcatacag acaaacctgt cttggattaa gatccccggc
     2761 ttcaacaaca atcttatcaa atatttcgcg gctcatatat ccgcgtttaa tctgatttcg
     2821 tacatgcgcc ggcgcacaaa cagggcaggc acagttacaa tcgcttgtta attcaataac
     2881 cagcaatccg caatgttcga tagagaatcc gcttaattta tgaaacactt tggacaatgt
     2941 tttttttaca gaaaccataa agtatcagcc ggatataata ttttataaaa attttatttg
     3001 cccaaaatcg atcttgcctt caccgtcaac cagattggaa tcggcatgaa cgtgctctac
     3061 cttacccaca aacatttcat gagaaccggt aacaattgaa tccacaatag tacattcaat
     3121 atttaccggg caatcagcca gaatgggggc attcaccttt attccctcag aaagcttgat
     3181 gctctttttt aaaagtttat cttcatctct tttgctgaca cttcccagat aatcgaaatt
     3241 ctctttatat gcctcatcaa ccagattaac gacaaagcat cctgactctt ttatcatttt
     3301 gtacgaatgc cgcgacggaa caatgccaac catgaccatt ggcggatcgt aactgcagtt
     3361 acagcaatac cccacggcca gcgcgttgtt ttctccgtca agacctctgc acgacacaag
     3421 aacttttgat gccggttgaa gacacgattt gaatactgct tcttttttac tcatttttat
     3481 ctcccgtatt tcaatataaa attattttta tttaacaatt tgcataatat ccaataacga
     3541 actaatctca taggttggaa tttcttttga attattaaac tttcgtttcg gattgtacca
     3601 gcaggaatcc atactgactc tattagctcc accaatatct gacacaagag agtctccaac
     3661 cattaaacaa tcatcaggtt ttaccttgag atcttccgta atggttttaa aaaaaactgt
     3721 acttggttta atgcagccta tttcttcaga aatatattgc ttgaaaaaat atttattcat
     3781 atcgttcaat cttttttttt gaacatcaac aattccgtta gttgcaatga ctaattttat
     3841 gtcttttgat agtgcctcgc aaatatccag cgcacagggc atataattta tttcagtgga
     3901 tagcttttgc aaatataatt cactcatgtc agccgccgat attgaaattc ctgtattttt
     3961 tattctttca aatctattta tagaatattt ataatacaat ttatggtaat tcttttgaat
     4021 aattatatca tccgacttgc caagattcag cctgtcccac tctttcgcgc atatttcttt
     4081 aaatttggtt atcaaatcct catcgcaatt gacctcatat tctttcatcg tattaataaa
     4141 cgcattagat tcagacctgt caaaatccaa aagtgtgtta tcaacatcaa ataaaataaa
     4201 tctgtatctc atagcgttac cataaaaatc tcaccggaaa acaggaaatc aataagaaca
     4261 aaacctgtat acagccttta tattctttta caaactgtaa agaataattc tttgcttgac
     4321 tactggttat acttttatat gaatatctgc gattaaatat ttttatctca aggagagcct
     4381 gatggaaaac gtttttgaca gaagaaccgt cgaacaggga caagccatta taagtgagcg
     4441 gttttacaat tttattatcg gcctgaccct ctgctggggt ttcggtatta attaccttat
     4501 ggtcaaacat gtttcaccag gttttgcttt gcaatacgga ttttggccgt ttattattgc
     4561 ctactttatc atgtgttttt tgggaatata tctttttaca aaatatgatg aaccgcttat
     4621 cagtttttta ggatacaact tggttgtggt accctttggt tttgtaataa atatcgtcgt
     4681 atcgcaatac agcccttcac tagttctcca agccgtacaa atgacaggta ttgttacgtt
     4741 aattatgatg tttttgggaa cacttttccc cgcgtttttt aaaaaaattg ttggtgcatt
     4801 aacaatcgca ctttttgcca tgataattgt cgaactggtt gcagtatatt tttttaaaac
     4861 tcatcacaca atcattgact ggatagtagc ggcaatcttt tgcggataca tcggctacga
     4921 ctggggacgt gcccagcaaa ttcccaaaac agttgacaac gctgtagaca gtgcggccgc
     4981 tctttatatg gacataataa acctgttttt acgtatacta agaatcatgg gaagacgcag
     5041 gtgaaagaca taatgataag ctctgaaaat taacttttca atctgtaatt gaaattttat
     5101 cgttatgaga cgctccaata atattggaaa acaaggactg ataaacatta ttttgtacaa
     5161 aaaggattgt gaggcattat ggaattttta cctttaaaaa ttgatggtgt aaaaacatac
     5221 gcaggatttt ggaaacgatt tctttcagga ttgattgatg ccataatttt tatacctgca
     5281 atctttattt ttaattattt tatcaagaca tcgatacctg tggcaatatt gataacagta
     5341 attcaattat ctctttatac gatatattct atttatttta attactacta tggcgctaca
     5401 attggcaaat caaacaaaat catttaaatt aattttatat cccgaacaaa aatttaaata
     5461 gtttatcttc caatcgccgt tgtttgataa attttatcaa atccttcgtc aaatcatatc
     5521 ctaacttatc gccttcttca ttaaatattg tctttgcttt ttttaaatca ctaagactgc
     5581 cattataatc atcatattga tcatgcttta tttgacctcg tacataatat gcccatccac
     5641 agttaatatc agcctcaata gctttatcca tatccttaat tgcatcacta taattattac
     5701 ggaaagattg aacacgtcct cttagcaagt aagccttgca tattttatat tttaattcaa
     5761 tcatcttgtt tgcatcatca tacgcttgat cgagcatttc tttcttatag ttaaaatagt
     5821 tatacttaga gtatccacgc aacaagtatg cctcaccaaa atcaggttta ataataattg
     5881 cattatctag atcttctata cctttgctat aatgacctat acctatatat aatcgtcccc
     5941 gctctaaata agcctctgca tcattttgat ttattgcaat tttatctgat aaataagata
     6001 ttttgctgtg ataatctccg taattgtctt taagatgctt ctgatagaaa taccgctccg
     6061 aacatgaaaa caaaaataca acttgagtga taattatcaa aaactgtttt aatacctttg
     6121 ataattttgt cttttggtta gatttgattt aaatacccct acacttcgcg tatcttaaac
     6181 ctggtggtca tctctttttg gctggctgcc atcttggcaa tatcaccaga ggatgcggcg
     6241 atctcttctg ctcccgagct tatagactgt gtcagctcat tgatacgcga agtcgactcg
     6301 acaatctccg ccatagcgcg cttctgttcg gctgtggcaa tttcgatgtt ttcggacttt
     6361 tctttaacct tacgcaattc atcatctacc attttataac tttcgacctg acgaatcgtg
     6421 taaccagaaa tcatccccgc catttctgca atattggcaa caccatctat aatctttttc
     6481 atttcgttaa taacttcgag tacatactgc ttgccttccg agatctccga gtcgctcttt
     6541 ttgattaggg tgtctatctc tttaacgctt gtagctgtgc tttcggcaag ctttgatacc
     6601 tcatcggcaa caactgcaaa tcccatacca gcactacccg cgcgtgccgc ttcgattgac
     6661 gcattaagcg ataaaaggtt tatctgctca gagatatcat ttatcatact tattatcgcg
     6721 cgcatctgtt cagagctttt gctaatatac tgcatattgc tatccatgcg cgaaagtgac
     6781 gcttcacccg accgcgcata ggtgctaata agttgggaca tcttttttga ttcggaagcc
     6841 tttacttcgc tctcattaat tatctgtgca agctcctgaa tcctttcggt caagactgta
     6901 aggctcttaa tctgatctgt agtgcccgat gagattgtat ccattgaagc agacacttct
     6961 tcgattgttg cactaacctc ttcgactgtc gcggcctgat tttgtgtatc tgacgaaaat
     7021 gtttcggtaa ttgtcgatat atcatcagtc gatcgggtaa gtacatccga agccttaata
     7081 atatcagaaa acatcccggc gacctgctct ttcatatcat agagcgttga gtttgcgccg
     7141 cttagctcat caattaccgg tgtcactttt ctattttccg aaaaatctcc agtacccatc
     7201 ctgtttacac tagagacaat caactcaatt ttatcacgaa ttgttctaat tagagccatg
     7261 cccaaactta aggatagtac aatcccaaaa ccgagtatgc ccatcaaatg cacccaggag
     7321 aaaaatcctg ccgacttgtc gtgcataagt acgataaaaa aatacccaat taaagataac
     7381 ccagacaaaa gaaaaatata tacgacaact attgttatcc taaaaaagaa ggtgatcgaa
     7441 aaatatcggt ttttgtcatc aatgcgaggg aaaacaccgg tgttaagaac tttttgtgta
     7501 agaatctctg tcatcaaaaa ataaaaaata aaaaatagac ccaaatggag ccattatcgc
     7561 aaccagtatc caagtatttg ttttttggat agaagtgaaa ttgtataaac tgagaaaagg
     7621 ggcaaactgc agagagaggc ctatcatcca tctaaagaaa gaaccaattg aatggtaaag
     7681 aggaaggtta aagaaccgat gttgcgcctc aagatacatc tcttcggaaa tattctgttt
     7741 attgattaac gccttaaaat attttgttac cggagcaagg acaatatggt tgttaatcaa
     7801 tgttgacgaa aatgatattg caacaactac caaggtaatg taaaacatca tctttaattg
     7861 ttcaaaatcc agttcaagtg agagaatgat gtttataaat aagatcggta caataataaa
     7921 ataactgata aattcggtat aaagaaaaaa ctttaacgaa aagtagcgca aaagacttga
     7981 atgcgaatcg acggttgagg ctgcaatctc tttatgaatg ttcatagtcc ctctatacaa
     8041 ctgttgttat ctagcactga atattaaatg atatcaaata acagcccaat attaaacaat
     8101 ggacggcatc agtattagag aaaagactgt gttgcgccat agtatctact attaggaacc
     8161 attataacaa aagatcttac taagtttgct cgacaaacaa taacaaactc tatagcctta
     8221 aaatttaaat tacaaggtgc taccaaaatg gtcaagataa aatcattggg ttacaattat
     8281 catttaaata atttaaccat taaatcaggt gatattagtt aaaccatttt tttttccgcg
     8341 cgctatatac actttttaca taatgtatat ataaagtgta aattattagc catgtataaa
     8401 ttattataat tgcgatatca gatcaaatta tcttaaacat cacttaaacg ataaaatgct
     8461 agtaaaataa tagttctaat tatttgaaag acaatactgt atcattttta acttagatta
     8521 taacaatatt attaataaaa taatgtggca attgaagaaa acacattgca ttatgtttca
     8581 ataattgtaa atatgacgtt atagtacccc agattaacca gaccaaactg acctttggga
     8641 gacaaccaat gaaccacgat attcttcctt ctgctgacac agagatcgag cgtttttatc
     8701 agtaccttca tatacttgca ttattacaaa atccaaacga gatagagcta aaacaaaaag
     8761 ccgagattta ctctttttta aaaattgatt ttgaacaacg ctatccacat tacgatgacc
     8821 agcacaagaa gacagcaaaa tttatcactc caaagaacat agaaaactat ataaaaaata
     8881 ctattctaaa caacgaacac ctaaatataa gatcagataa aaaacgttat gtattaaaca
     8941 cgccataccg caaagaatcg cttctgccct ttttacgatc ttataccgcg atcacggtaa
     9001 cagacgcggg gaaacaggat gtacttttaa agataatcaa aagccataaa aaccggttta
     9061 tgtggaatct tgcgacagtg cactttgcaa ttatagaacg ccggatacta tcaattgact
     9121 atttgaagtc ggctaacgct gacactaaaa cgtccgaaat tgtaccgcat catcttgttt
     9181 atcgtaacaa taatttatat cttataggcc gggttacagg taaagataac gatgcccagt
     9241 ttatttatac gcatataaaa aaaatcaaac cgaccgataa aaccacagat ttaccaatta
     9301 ctcctctgga agacatatac aaagatacta tcggctcttt ttttaacatg ggtaaagaac
     9361 cgcataacgt aaaaattcgc tattgctctg atatcaaata cgatatggaa cgacttctaa
     9421 aaaacataga agcggatata acagatataa gcaattttga cagcttatac gactacgagg
     9481 caaggttcaa attggcagag gatacatttt tttgtaagca gattttcggt tttggtaata
     9541 aggctctgat catagaaccg gccgacctta aagagatgat gataaataca gcaaaaaaaa
     9601 ttactaacca gtatgtcact gcacataaat ctaatcaaag agaccacaaa tgattaacaa
     9661 ttccaagata aaagataagg tgtcatcaga cgcaaataaa gataccagag agttactctc
     9721 aaaacccaat gttccaaatg atccagcaac aaaaataagg atcatggaac gttacctgca
     9781 tatacttgca cttattcaaa accctgaagg tatcgatggt ggctggaccg acaaaaagat
     9841 tgctgataga ataaacgaag tacttgaaaa caaagaaggc agtgatgatt cactcaccga
     9901 tgacaaggtg aaccgtgata ttaaaacact tcgagatgat ttaaatatta gcattagcgg
     9961 tcagggaacc agcagaagaa tgattatgga tactcttgat ccgcatctgc aaattacact
    10021 tcttgaagcg tacgcgcctt ttgtaataac agacaacagc cgggatttga tacttgataa
    10081 tattgtccaa aacaattccg attattgtgc ctggaatata gccgcggtta aatacgccct
    10141 tacaatgaac agatactgca ataatatctc agctacctat acgttaaatt tcgattatgc
    10201 atcaaaagct tgtaatcaca caaaagatga aaacagagaa attgtcccat accatcttgt
    10261 tttcaaaaac aataatctat atttgatcgg cctcatgtta agcaatggtt tacccgtaca
    10321 taactctcca acagtttatc tgtttgaaaa aattaaagag attaaactta aaaaatccga
    10381 caaaatcaca cacactgttc cacctgtcaa agagatttat aaaaacagca caagctcatt
    10441 tattaatcaa aaaacacatg atgttgttat acgatacact caaagcgcac aaaccgagct
    10501 aaagcgcgta cttcccgaaa ctgcaattga aacagaaatc aaagatgcga acaattcaaa
    10561 gtacagctac gaaaccagat ttataagctg cgatgatatt tacttatgca aagagctgtt
    10621 tgtttttggt gaccaaatag agattattaa gcctaccgag ttgagaaaaa tgatgtttga
    10681 taatgcacaa gccgtacttg atgcctacgg tgtttgagtg ccttaaagat tcatcaatca
    10741 ccatcataaa atgtcataat ctgcaccatg cggttgggcc ttacttcgta aacatcaaca
    10801 gatacaccgt aaccgtaaac cgctttaatg actaactcca ttttaccgtc accgtttaga
    10861 tcaattggaa atatttcaag ccattccgca acgcaatttt gatagtaatc ataatccgca
    10921 ggaactgaat atccatccga atgcaaagtc tttccatgaa aaacaaaagt gacccttgct
    10981 gcgttgcatc cacctttatc cttgcatctt tcgaaaccta tcttttcgat ttcttcgtct
    11041 ccatccaaat ctacaagata attatttccc ttattaattg gtttaacggc tcgcggcaac
    11101 gcattccagg ttccattaac tcctataagg aaggatctgg cacaagaaat cggtttaaaa
    11161 aatgtaccga acatcagatc cgaagtactc cactgagtat aaatccctgg gttttttatc
    11221 tttgagcttc caattcgttt attaaacgca taaaatacga aggtttcgtc acctttggca
    11281 agatctattt gtcctaaatc tgtttcgttt tttacaggat ttaaatgtag agcatcttta
    11341 tatttatcgg catccatttt ttttcccata aaatggatat caaaatcatc aagagaatac
    11401 cataatttat ccctggatcc acccacaatg taacctgctg aaaaaacata ttcacgactt
    11461 tcccatgcga ttaagactgg atgatatcta tttactactc caatattctc ttctttcaaa
    11521 ggctccatat tccggttttg ttgagtagat ttttgtccac atcctgtgac agctgttaaa
    11581 ataaatattg cgaacaacaa tgtcgttttt ttcattaatg gccccagttt ttcaaaatga
    11641 agaaaaaacc ttatattaaa aaaattgttt tagtaatttc aagcatatta tcacgtgatg
    11701 atattttttt atttccaaaa tttatgactt gaaactaaca agtattcagc aaattaatac
    11761 actggttaat tttaaaacca cacacaattt aagaggtgcc atgatatccg acaagatgat
    11821 ttcagctgta ctcgaaaacc aacagcaagc aattaaaagc ggcaaccctc catttgcggc
    11881 aataataaca gtggacgatc aaataatcgc tacaagctat aatcaatcac gatcgtcagg
    11941 taatccgctt aaacacgcag agatatcggc gattgaatct gccatcaagg ctcatggagc
    12001 atctatatta caaaaagctc atctaatttc atcaaacgaa ccatgcccca tgtgtgttgg
    12061 cgcctgtata tggtccggca taccaaaagt aacctacttt atttcccagg aacaggttgc
    12121 tgctattcgc ggatggggaa aatttatgcc ggcacacgat attgctgcgg cagatgattc
    12181 aggaatagta atcaatgggc caattgataa tgatgaaatg ataaaatttc atcaagattt
    12241 ctggacagca gacaacaact ctcaatacaa acattctatt catttgtccg gatctgtaaa
    12301 aactgaaaca taaaacacgg tccaattaag caagccggca aaatcgggct ttaacataac
    12361 gtttataaat acgacacaaa aaaaataatc actggagaca gttatgaaag aatacgtatt
    12421 cccatatttc ggaaaactac agatggatca tttggaagag tattacgatg ttaatattga
    12481 ttacggtgat tctgaaattc aaattgaact tactttcgaa aacaaaacaa ttgagaccta
    12541 cattatggat aaaacaaaga accttattga aaatatcagt aaatttgata aacagaacca
    12601 gatatatatt tttaacgatt acaataatag tgacggcgac accgtaaaaa cttatctgca
    12661 gcatcatctt gaagcagctg acaaagaaga gctgtccaga ataataaact ttgaagacac
    12721 taaaatcaaa caggaaca
//
LOCUS       DAOTGZ010000062        12680 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_3064
            NODE_5920_length_12680_cov_5.198257, whole genome shotgun sequence.
ACCESSION   DAOTGZ010000062 DAOTGZ010000000
VERSION     DAOTGZ010000062.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868088
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 12680)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 12680)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:22:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,785
            CDSs (total)                      :: 2,751
            Genes (coding)                    :: 2,732
            CDSs (with protein)               :: 2,732
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 12 of 19
            Pseudo Genes (internal stop)      :: 5 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12680
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5920_length_12680_cov_5.198257"
                     /isolate="AMR_MDS_3064"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1347
                     /locus_tag="P5123_05795"
     CDS             <1..1347
                     /locus_tag="P5123_05795"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HRX15805.1"
                     /translation="FMMSTAIITVLLIMSPGPAIIGIFTVGLLYSLFFAVVKNYLKKI
                     GIERIIYNEIRFKTANEAFGGIKDVKLLHKENYFIKTFSNSARAYETRMANGKIIATM
                     PHYILEGLAFGSILAISLILFAIHESINDVLPLIAFYTYAGYKIMPNFKEIFKSVSTV
                     RKQISALDLIYRDLRDISDIRLISLQKQEEKKMEPISFKESICLDNVVFNYENNNARI
                     ADNLSLTIKANTTVAFFGTTGCGKTTIIDIILGLLIPKSGKLLIDGTELNEKNIYSWQ
                     RKLGYVPQNIFLVDDTISANIAFGIPRDQIDMNAVRTAAHIANMDHFIENNLPSSYET
                     VVGERGIRLSGGQKQRIGIARAVYHNPDVLFLDEATSALDNATEAAVMDAIDNLSKNK
                     TIILIAHRLSSIKKCDTIFFLDKGKIITSGTYNELLQNCEEFKKIAMDISPLPASV"
     gene            complement(1356..1712)
                     /locus_tag="P5123_05800"
     CDS             complement(1356..1712)
                     /locus_tag="P5123_05800"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRX15806.1"
                     /translation="MDLFFWFCLITIVVTAIVSAVHLLVKNSGLYITVPLWIIKIPVI
                     LMNLVMLFFTFVGFGRLGIIIFILLGAIVFHQVKFLLNLKKTDSKRFQLFRDIMLLLT
                     PFAVIVSTVVIRQIVL"
     gene            complement(1869..3212)
                     /locus_tag="P5123_05805"
     CDS             complement(1869..3212)
                     /locus_tag="P5123_05805"
                     /inference="COORDINATES: protein motif:HMM:TIGR00797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0042910 - xenobiotic transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006855 - xenobiotic transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MATE family efflux transporter"
                     /protein_id="HRX15807.1"
                     /translation="MKLNKTMDQRMTIFALSWPLLIETFLRMLFNNIDVMMLSRYSDD
                     AVAAVGLIGQLMFMVIILFMMVNTGAITLISQYNGSERYNESSKITAAAISLSAVFGI
                     VATLILTLGANPILGIYDLEPAVRGYAYDYLFIFGLFCVGTALSTVFAAILRSYGYTQ
                     SAMVVTFGCNVINIIGNYLFIFGPFGIPVLGVKGVAISTVASNCINAVIMLYIISTKK
                     EIGLNIKSITSVTVESYKKILNFGLPNGGENLSYNLAQLVSYKVIAISGTAALTAAVY
                     TQTFQRFIMIIPFALMQSSQIYVGYLVGKRRFDEAYHKIKNVWYFVFSVALVASIGVA
                     YFRHELIKIFTEDPSVASIAVVLMVAAILWETGRTFNLIFIGALKGAGDVHFPVRAGI
                     VSMWGIAVFFSYLLGLKFGLGALGVWLAFSMDEWGRGLVMFFRWRSRAWETKRVE"
     gene            3455..4003
                     /locus_tag="P5123_05810"
     CDS             3455..4003
                     /locus_tag="P5123_05810"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRX15808.1"
                     /translation="MKRIIILVFTIFLITSCGSTSTRTEYPDAKESYSPEAAYVYGKF
                     TQVITTDNYRWPYIYIYLQGDDKKEYLYIYSSHKDNEIYQITGIKPGTYTIQGVGATG
                     RNLKGYIEFKKTDLGKKITVEAGKAYYIGDYTTFYDSKTSRFGLYNYEDAYRKTTSEI
                     KKKYPLLKRIKTAKVNPKKPRK"
     gene            4283..5056
                     /locus_tag="P5123_05815"
     CDS             4283..5056
                     /locus_tag="P5123_05815"
                     /inference="COORDINATES: protein motif:HMM:NF013315.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HRX15809.1"
                     /translation="MKKESYYDRKKKFIREIADLGIRKEIIKHFSDTEISVFFDSLFE
                     DRFYSDQLIPIGFGEYSDDPLNLAKMINKLNPDKKGRILEVGTGSGFSTAILSMLYRE
                     VYTIDICEDLAITAKNRLYNHGYENIRFYAGDASIDTAEIDELFDGIIIHASCFKRPF
                     TIISLLKEKAPIVFPMGPPTQQQIITLYNEPSPDGEGNFKTHFLDFVNCTPIQGPYGY
                     DTPNIPDVYRDMDDEEDEEDEDGKVKSDTNTKKTDQTKK"
     gene            5053..5943
                     /locus_tag="P5123_05820"
     CDS             5053..5943
                     /locus_tag="P5123_05820"
                     /inference="COORDINATES: protein motif:HMM:NF013088.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HRX15810.1"
                     /translation="MSQTTRGYLYLFVTILLFSTYEVVSKTLIGSVSPLQINVYRFFL
                     GGFLLLGYLIYKRNTSITAYHLFMCTLVGIINVVISMNLVLLSLWIEGGRASVTAALI
                     STNPLFVLIIASLFDKEKITGKNITGIILCIAGVAFIFDDTFTSTGSYQSALLALGSS
                     LSFAIYTVMGKKLTSRIGSSTKMNCYSFLTGATVLLIVMLIIKSPVAIKSVSAFPQLV
                     YLGIMVTGVAYIAYFKGLTITGAGRGSLLYFLKPVIASLLAIIFLAEQPTIGFVAGTA
                     LILAGLYIAILLRWPRPDLE"
     gene            6118..>6462
                     /locus_tag="P5123_05825"
     CDS             6118..>6462
                     /locus_tag="P5123_05825"
                     /inference="COORDINATES: protein motif:HMM:NF013836.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRX15811.1"
                     /translation="MISFPGYDDIIKRVYEEKQEHVFRFWDAIDDNKKKSLLEDLKNV
                     NFEEINRQYLNASAESVIDEDYGPADYIRLPRNQEESAKFTQARETGVEAIKKGRVCA
                     FIVAGGQGSRLGY"
     assembly_gap    6463..6472
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <6473..7506
                     /locus_tag="P5123_05830"
     CDS             <6473..7506
                     /locus_tag="P5123_05830"
                     /EC_number="2.7.7.9"
                     /inference="COORDINATES: protein motif:HMM:NF013836.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070569 - uridylyltransferase activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="UTP--glucose-1-phosphate uridylyltransferase"
                     /protein_id="HRX15812.1"
                     /translation="GPKGAFPFTPIKHKSLFQVFSEKILKSSKIYDVSIPLLIMTSHA
                     NHQQTKEHFEQNKYFGLDPENVFMFQQDMIPSLTTSGKLILASQNSLFRNPNGHGGSL
                     IALKKSGMLQKLKEKGIDLISYFQVDNPIVCAIDPVFIGFHILNNAEISSKALTKAYP
                     QEKVGNFVIFPGKKLGIVEYSDLPEDKAYEKESDGSLHYSAGNVAIHIFSVDFISKIT
                     SDDFSLPYHLAEKKILAYNNGEPEKIDGYKFEKFVFDALPLTDKNVILETSRRNEFAP
                     VKNKTGLDSVDSSRQLMMDMHREWLEKRGIEIPDSVAIIEISPLKALRAEDLNKDIII
                     PNQSEVYLD"
     gene            complement(7503..9008)
                     /locus_tag="P5123_05835"
     CDS             complement(7503..9008)
                     /locus_tag="P5123_05835"
                     /inference="COORDINATES: protein motif:HMM:NF019205.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase dimerization/phosphoacceptor
                     domain -containing protein"
                     /protein_id="HRX15813.1"
                     /translation="MNRVLYHKNTGASLEGASGILPLAFILVSAGVLYYYDLSLFVFS
                     VKAFSVIITFAVSMVALSTNRQYISGFIAFIALACFFVATIEAFTMVYSVKYIINEDA
                     DNRITFWFHSANWVFLSAFVGAYLYTGIKKKLAVWKLQIAYGIVSVAILAFPLLITLE
                     KNINQNSYLHVSTLFLFTAWSLSALLYSGLRRSYPSESIYLTVTVILLNALSTALLQF
                     SEDQNGGFSAMAVFFRFAAMCMLLWLVINHKILHKKESGVLKNEKSDFDNAVKHMVDE
                     RLRDLNAKNSQLKTIMRQKNIAAKEACFHTKNNLQIISSILDLRDDIPKKHHADIQAK
                     IQSIALIQQMIYRSENSRQFEIKSYLKRLSGLILKIYNTKKDININISGPDIDVDLDT
                     ALPIGLISNELIANSIQHAFPESERGNIDIEICRRNHEALQITYRDNGKGLPEDFTLE
                     TNGSYGMRNIESIVVDQLRGNMRICKENGFSIKIIILVFHNDVIMGALFVH"
     gene            complement(9218..10198)
                     /locus_tag="P5123_05840"
     CDS             complement(9218..10198)
                     /locus_tag="P5123_05840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019027381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HRX15814.1"
                     /translation="MEKVNWGIIGPGRIAESFAKGIAFVDNAVLHSVASSRKERADAF
                     AGKYSVKNSYGSYSEMLDDPGLDAVYIATPHSFHYDNVKLCLNAGKSVLCEKPLTINE
                     KMGRELVDLARSKNLFFMEALWSRFLPIYTDVRDWLDGSRIGDIRLICANFGINVPYD
                     LNDRWYNPDLAGGALLDLGVYPIAVTQWILNENPVTVKAEAVMTETKVDQLTAGVLKY
                     PSGVIMQMNCGFAAQALNDLKIYGSKGSITIHHCFWATDSATLETEDNKLTVTRPFKA
                     SGFEYEIGAATKAIIEGKLECSGITLDDTLGTLRVMDTIREQIGLKYPFE"
     gene            10769..11275
                     /locus_tag="P5123_05845"
     CDS             10769..11275
                     /locus_tag="P5123_05845"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRX15815.1"
                     /translation="MNNGQKHPRSITIPDVEETEKLILKLKELSSFIIDTSSLIYMQK
                     ISVMKMVTKIYNIKAPLSVINEFGSCPEGITTVDINLSADDDIIACADQFGLPVCSED
                     KRVLLHASRDNIDYYNTIMILVSMRTKNIITKQEFGQYITGLRSNAHYSNKVWDYAQK
                     LIDTVDMV"
     gene            complement(11498..11956)
                     /locus_tag="P5123_05850"
     CDS             complement(11498..11956)
                     /locus_tag="P5123_05850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009147094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PIN domain protein"
                     /protein_id="HRX15816.1"
                     /translation="MKIFKIYIDTSVFGGVFDQEFSEYSLLLFNKFRKNEYLPVISDI
                     TIAELENAPDRVKNLLLDFDDLELIEADNEMKKLAAFYLDSEIVSPKYSDDALHIAIA
                     TICKVDVLVSWNFKHIVNLKKIHQFNSVNLREGYNLLEIRTPREVISDER"
     gene            12065..12526
                     /locus_tag="P5123_05855"
     CDS             12065..12526
                     /locus_tag="P5123_05855"
                     /inference="COORDINATES: protein motif:HMM:NF024304.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxamine 5'-phosphate oxidase family
                     protein"
                     /protein_id="HRX15817.1"
                     /translation="MRRHDKEITDQDTIKQILKENTVCRIALCDNDTPYIVPMNYGFD
                     EINIYLHCAKEGKKLDIIKENSRVCFEVTDSAETVTSDKACGYGTRYRCVIGQGRIAL
                     VDSAEEKIQALNIIMKQHTGKADWDYDPSVVEKITVLKITAESITGKISGF"
BASE COUNT         4302 a         2521 c         2223 g         3624 t
ORIGIN      
        1 ttcatgatgt caactgcgat aataactgtt ctgcttatta tgagtccggg accagcaatt
       61 atcggaatct ttacagtcgg tctattatac agcctttttt tcgcggtagt taaaaactat
      121 ctgaaaaaaa ttggtatcga aagaataata tataatgaaa tacggtttaa aacagccaat
      181 gaggcgtttg gcggtatcaa ggatgtcaaa ctgcttcata aagaaaatta ttttataaag
      241 actttttcca attcggctcg agcatacgag acgcgcatgg caaacggcaa gataatcgca
      301 accatgcccc actatatttt ggaggggctc gcttttggtt ccatactggc tatatcactg
      361 atacttttcg ccattcacga atccattaac gatgttcttc ctcttattgc cttttatacc
      421 tatgcgggct ataaaatcat gcccaatttc aaagaaatat ttaaaagcgt ttcgacagtg
      481 cgcaagcaaa taagcgcgct tgatctgatt tatcgtgatc ttcgcgatat ttcggatata
      541 cggcttattt cgttgcaaaa acaagaagag aaaaagatgg aacctatctc ttttaaagag
      601 tctatttgcc tggacaatgt cgttttcaat tatgagaaca ataatgctcg tattgccgac
      661 aacctttcac tgacaataaa agccaataca accgttgctt tttttggaac taccggatgc
      721 ggcaagacta caattattga tattatatta ggtcttttaa taccaaaatc gggcaaattg
      781 ctaatagatg gaaccgaatt aaatgaaaag aatatctatt catggcaacg aaaactcggt
      841 tatgtacctc aaaatatctt tcttgtcgat gataccatct ccgcaaatat agcttttggc
      901 atacccaggg atcaaataga catgaatgca gtcaggactg ctgctcatat tgcaaatatg
      961 gatcatttta tcgaaaacaa cctcccctct tcatacgaaa ctgtagtcgg agagagagga
     1021 attcgtctta gtggtgggca aaaacagcgg atcgggattg cacgagcagt ataccataac
     1081 cccgatgttc ttttccttga cgaggcaaca tcggctcttg ataacgcgac tgaagccgcg
     1141 gttatggacg cgatagataa cctgtctaaa aacaaaacta taatcctgat tgctcaccga
     1201 ctctcgtcca tcaagaaatg cgacaccata ttctttctgg ataagggcaa gataattacc
     1261 tcaggtactt ataacgagct gttacaaaat tgtgaagagt ttaaaaagat cgcaatggat
     1321 atctcaccat tacccgcatc tgtttgatac tattattata aaacaatctg tcttatcaca
     1381 actgtcgata caataacagc aaagggagta agcaacagca taatatctct gaacagttga
     1441 aatctttttg agtctgtttt tttaagattt aaaagaaatt ttacctgatg aaacacaatc
     1501 gcacccagca aaataaatat tataataccc aatcgtccaa agcctacaaa agtaaaaaag
     1561 agcattacca gattcataag tataaccggt attttaatga tccagagagg aaccgttata
     1621 tataatcctg aatttttgac cagcagatga acagcactaa caatagctgt aacaactatc
     1681 gtaattaaac aaaaccagaa aaatagatcc atcagtaaac tcctataaat tttgaattgc
     1741 ctgtcaaagg tgacaggctt tcgtttattt tgcaatcttt attaagttgc gttataaata
     1801 acaaaaagtg atcgcataaa ttggtccaaa aacgatgcac taaatataat ctgctgagat
     1861 accaaatgct attctacgcg cttagtttcc caggcacgtg atcgccacct gaaaaacata
     1921 acaagccctc gtccccattc gtccatcgag aaggcaagcc aaacacctaa ggcaccaaga
     1981 ccaaatttaa gccccaaaag ataagagaaa aaaaccgcga ttccccacat ggaaactata
     2041 ccagcgcgaa caggaaaatg gacatcaccg gcacctttga gagccccaat aaaaatcaga
     2101 ttaaaggttc gcccagtctc ccaaagaatt gccgcaacca taagcacaac agctatagat
     2161 gccacacttg gatcttccgt aaatatttta attaactcat gtctaaagta tgccacacca
     2221 attgacgcaa ccagcgccac agaaaaaacg aagtaccaga catttttaat cttgtgatac
     2281 gcttcatcaa accggcgctt gccaaccaga taccccacat atatctgaga agattgcata
     2341 agcgcgaacg gaataatcat aataaaacgc tgaaaagttt gtgtataaac agcggccgta
     2401 agagccgcag ttccagaaat agctataacc ttatatgaaa ccagctgggc aaggttatac
     2461 gagaggtttt ctccaccatt tggcaaacca aagttcagta tttttttata cgattcaaca
     2521 gttaccgaag ttatagactt tatgtttaaa cctatctctt ttttggtgct aattatataa
     2581 agcatgatta ccgcgttaat acagttactt gccacagtcg agatagcaac acccttgact
     2641 ccaagaaccg gtataccaaa cggtccgaat ataaaaagat agttgccaat aatatttatt
     2701 acattgcaac caaatgtcac aaccatcgcg gactgggtat agccgtaaga acgcaaaata
     2761 gccgcgaaaa cagtactcag ggctgttcct acgcaaaaaa gcccgaagat aaaaagatag
     2821 tcataagcgt atcctcgtac agccggttca agatcatata tacccagaat tggattagcg
     2881 cccagtgtca gaatcaaagt ggcaacaata ccaaaaactg cactcaaact tatggccgcg
     2941 gcagttattt ttgatgactc attatagcgc tctgatccgt tatactgtga tataagtgtt
     3001 attgccccgg tatttaccat cataaaaaga atgattacca taaacataag ctgcccaatc
     3061 aaaccgacag cggctaccgc gtcatccgag taacggctaa gcatcattac gtcgatattg
     3121 ttaaacagca ttcttaaaaa agtttctatc aaaaggggcc acgacaaagc aaagattgtc
     3181 atccgctggt ccatggtttt gttcaatttc atagccatac ctcacaacag ctttaagtaa
     3241 aaacacttaa cttattttat aaaagaacca tatccaattt tatattttgc ttgtacactt
     3301 ctttatgata agaatgaatt tttatttata agcaattata taaaacctgc atacagttgc
     3361 agaagatccg aataaagaaa ttttgttctg taaatttgaa ttgacaaatc ttccagatag
     3421 tgcataattc tttaaaaaaa cttagaagcc aaaaatgaaa agaatcatca tacttgtttt
     3481 tacaatattt ctgatcacat catgtggttc gacatcaaca cgcacagaat atccagacgc
     3541 taaagagagc tactcacctg aagcagctta tgtttacgga aaattcactc aggtcataac
     3601 cacagataat taccgttggc cttatatata catatattta cagggcgatg acaagaagga
     3661 gtatctgtac atttactcaa gccataaaga taatgaaatt tatcagataa ctggtattaa
     3721 gcccggaaca tataccatac agggcgttgg agctacggga cgaaatctta agggctacat
     3781 agaatttaaa aaaacggatt tagggaaaaa aatcactgtt gaagcaggca aggcctacta
     3841 tatcggagac tacaccacgt tttatgacag taaaaccagc cgtttcggcc tatataacta
     3901 tgaagatgcg taccgtaaaa caacaagtga aatcaaaaaa aagtatcccc tgcttaaaag
     3961 aataaaaact gcaaaagtta atcccaaaaa accaagaaaa taattcactg aaagtcgcat
     4021 aaatgcaaat aaatcacgca ttgtttctaa acagcagttt ttgaaacaat gcgtgctctc
     4081 ccccaaaagc acacaaagat aattaaaaaa aatttagaat aattacctca ttgaagacaa
     4141 ttcagctgat ttcaatcacg aatatttaat cagtttttag caaaatgtta aaaaaaataa
     4201 attgcggcaa aaagataaaa ctggttaatg gtcagaaaca gagattaatg aaaccggttt
     4261 cttccggaaa aatggcaatc agatgaaaaa agagtcgtat tatgaccgca aaaagaaatt
     4321 tattcgcgaa atagccgatc ttggaatccg aaaagagatc attaaacatt tttctgatac
     4381 agaaatatct gtcttttttg attccctatt tgaagacagg ttctattctg atcaactgat
     4441 accgataggt ttcggtgaat atagcgacga tccgcttaac cttgccaaga tgataaacaa
     4501 gcttaatccc gacaaaaaag gacgtattct tgaagtgggt accggttcag gtttttcgac
     4561 cgcgatactt tcaatgcttt atcgtgaagt atataccatt gatatttgtg aagaccttgc
     4621 cattacagca aaaaacagac tctataacca cggttatgaa aatatacgtt tttacgctgg
     4681 tgacgcgtca atagataccg cggaaattga tgaacttttt gacggaataa ttattcacgc
     4741 atcctgtttt aaacgcccct ttacaataat atcgctactt aaagagaaag ctcccatagt
     4801 ttttcccatg ggcccaccaa cccaacagca gataattacc ctttataatg aaccctcacc
     4861 agatggagaa ggtaatttta agacacattt tctcgatttt gtaaattgta cgcccattca
     4921 ggggccttac ggatacgata caccaaacat acccgacgtc tatcgagata tggatgatga
     4981 agaagacgaa gaagacgaag acggcaaggt taaatcagac acaaacacaa aaaaaactga
     5041 tcaaacaaaa aaatgagtca aaccacgcgc ggttatttat atctttttgt cacaatactg
     5101 ctgtttagca cctatgaagt ggttagcaaa accttgatag gatctgtttc gcccctacaa
     5161 ataaatgttt atcgcttttt tctgggaggg tttctgctct taggttacct tatttataaa
     5221 agaaacactt caattacagc ctatcatcta ttcatgtgca cccttgtcgg aataattaat
     5281 gttgtaatca gcatgaacct tgttctcctt agcctttgga tcgaaggcgg aagagcttct
     5341 gtcacagccg cgttgatcag cacaaatcct ttatttgtat tgattattgc ctcactgttt
     5401 gataaagaaa aaatcaccgg aaaaaatatt acaggtatca ttttgtgcat tgcgggtgta
     5461 gcttttatat ttgatgacac atttacctca accggatcat atcaaagcgc attactcgct
     5521 cttggctctt cgctctcttt tgccatctat acagtaatgg gcaaaaaact gacttcacgt
     5581 atcggcagca gtacaaagat gaactgctac tcatttttaa ccggagcaac agttttactt
     5641 atagttatgt taataataaa aagcccggta gcaatcaaaa gcgtttcagc ttttcctcag
     5701 cttgtttatc ttggaataat ggtcactggg gtcgcttata tagcatattt taaaggttta
     5761 acaataaccg gagccggcag gggctcactt ctctattttt taaaacccgt tattgcctca
     5821 ttgctggcaa taatattttt ggcagaacaa ccaacaatcg gttttgttgc agggaccgcc
     5881 cttatcctcg caggtttgta catagctatt cttttgcgat ggccacgacc tgatcttgaa
     5941 taaaacatca aaaaacatct cattaaattt atataagata ttaacgattc acccggtctc
     6001 aatgttcttg acttattatg gctctgatta aatagtatgc ataatggatt tctttgcgaa
     6061 cacaaatcaa gttttcagct tttcaattag aacataataa tatcaggagt tttgataatg
     6121 atctcttttc caggatacga tgatataatc aaaagagtat acgaagaaaa acaagagcat
     6181 gttttccgtt tctgggatgc tatcgacgat aacaagaaaa aaagtcttct tgaagacctt
     6241 aaaaatgtaa attttgaaga aatcaaccgt caatatctaa acgcctctgc agaatccgta
     6301 atcgatgaag attatggccc agcagattat atcagacttc cacgaaacca ggaagagagt
     6361 gccaaattca cgcaggctcg cgaaaccgga gtagaagcaa taaaaaaagg ccgagtttgc
     6421 gcttttattg tcgccggggg acagggctca agacttggtt atnnnnnnnn nnacggccct
     6481 aaaggtgcgt ttccttttac acctattaaa cataaatccc tttttcaggt ttttagcgag
     6541 aaaatactga aaagctcaaa aatttacgat gtaagcattc ctcttcttat catgacttct
     6601 catgctaacc atcaacaaac aaaagagcat tttgaacaaa acaaatattt cggactcgat
     6661 ccggaaaatg tttttatgtt tcaacaggat atgattcctt cactaacaac cagtggcaag
     6721 cttatactcg cgtcacaaaa ctcgctattt agaaacccta acggacatgg tggcagcctg
     6781 atagcactca aaaaatcagg aatgcttcag aaactgaaag aaaagggtat tgaccttata
     6841 agctactttc aggtagataa tcccatcgtt tgtgcgattg accccgtttt tattggcttc
     6901 catattttga ataacgccga aatttccagc aaggcgttaa ccaaagcata tccacaggaa
     6961 aaagtcggaa actttgtaat ttttcccggc aaaaaactgg ggattgtgga gtattctgat
     7021 cttccagaag acaaggcata cgaaaaagag agtgacggat cacttcatta ttcagcaggc
     7081 aatgtggcaa tacatatctt ttcagttgat ttcatctcta aaattacatc cgatgatttt
     7141 tcattaccct atcatcttgc tgaaaaaaag atattagcat acaataatgg cgaaccagaa
     7201 aaaatagatg gttataaatt cgaaaaattt gtttttgacg cgcttccatt aacagacaaa
     7261 aacgtgatac tcgaaacttc aagacgtaac gaattcgctc ctgtaaaaaa caaaacggga
     7321 ttagattccg ttgactcatc tagacaacta atgatggata tgcatcggga atggctggaa
     7381 aaacgaggaa tcgaaatacc agattcagtt gcgattatcg agatatcccc tttaaaagcg
     7441 cttagagctg aagacttgaa caaagatata attatcccta accagtctga agtttacctg
     7501 gattaatgca caaatagtgc tcccattatc acatcattat ggaaaaccag aataataatc
     7561 tttattgaaa acccgttttc cttgcaaata cgcatattac ccctcaactg atcaactact
     7621 attgactcaa tgtttctcat tccataggag ccgtttgtct ccagggtaaa atcttccggc
     7681 aaaccttttc cattgtctct gtatgtgatc tgtaacgctt catgatttct gcggcatatt
     7741 tctatatcaa tgtttcctct ttctgattca ggaaaagcat gctgtataga gttggcaatt
     7801 agctcattgc taattaaacc aatgggaaga gctgtatcca gatcgacatc tatatccgga
     7861 ccgctaatat taatattaat atcttttttt gtattgtata tttttaaaat taaccccgat
     7921 aatcttttta aatacgattt aatctcaaat tgtctgctgt tttctgaacg gtatatcatc
     7981 tgttgtataa gtgctatcga ctgaatcttt gcctgaatat cagcatgatg tttttttggt
     8041 atatcatccc gcaaatcaag aatggaagat ataatttgaa gattattctt tgtgtggaaa
     8101 caggcctctt tcgcggcaat atttttctga cgcataattg tcttaagttg actgttcttt
     8161 gcgtttagat cccttaacct ttcgtcaacc atatgcttta cagcattatc aaagtcactt
     8221 ttttcatttt tgagtacccc agactctttt ttgtgtaaaa ttttatgatt aataacaagc
     8281 caaagcaaca tgcacattgc tgcaaaacga aaaaaaaccg ccattgcaga aaagcctcca
     8341 ttttgatctt cagaaaactg caaaagtgct gttgataaag catttaataa aattaccgtc
     8401 accgtcaaat agatactctc tgatggatag gatcgccgta aacccgaata aagaagcgct
     8461 gaaagactcc acgcagtaaa aagaaaaagt gtgctgacat gtaaatatga attctgattt
     8521 atattttttt ctaaagtaat taatagcgga aaagccagaa tcgccactga tacgattcca
     8581 taggcgatct gcaacttcca caccgccagc ttttttttta ttcccgtgta aaggtaagca
     8641 ccaacaaaag cagataaaaa tacccagttg gcggaatgaa accaaaatgt tatccggtta
     8701 tctgcatcct cgtttatgat atacttaacc gaatagacca tggtaaaagc ttctatagtt
     8761 gccacaaaga agcaggccag tgctataaac gctataaaac cggatatata ctgcctgtta
     8821 gtacttaacg caaccatgga aacagcaaac gtaataataa ctgaaaaagc ttttacagaa
     8881 aacacaaaaa gactcaaatc ataataatag agcacacctg cagacacaag aataaatgcc
     8941 aaaggcaata tgccagaggc accctctaaa gaagctccgg tgtttttatg gtacaatact
     9001 ctgttcatat taataagatg cattaataac agagccaagt caatattgaa tgtcaattaa
     9061 ttacaggctt acctattatt tggaattaat aatagttact ctggccttaa tgccgcactg
     9121 ttaacttata aaaaagcaca aactgtcttt tttgcgatag aaagagagca taaactttta
     9181 gccaataacg ttgtatctac tgtattcaaa atatattcta ctcaaaggga tatttaagtc
     9241 caatctgttc cctgatagtg tccataaccc taagtgtgcc cagagtatca tcaagagtta
     9301 taccggaaca ttcaagcttg ccttcaataa tggcttttgt tgcagcacct atttcgtact
     9361 cgaaaccgct cgccttaaac ggacgcgtta ctgttagctt gttatcttct gtttcaagag
     9421 tcgcgctatc agttgcccaa aaacagtgat gaatagtgat agagcctttt gaaccgtata
     9481 tcttcagatc atttaaagcc tgtgccgcga atccacagtt catttgcatt attacacctg
     9541 acggatattt taatacaccc gcagttaact gatcaacttt tgtttcggtc attacagctt
     9601 cggccttaac tgttaccgga ttctcgttaa gtatccactg ggttacagca atgggataaa
     9661 cccccagatc aagaagagct ccgcctgcca gatcaggatt ataccagcga tcattaagat
     9721 cgtagggcac attgattcca aaattggcac agataagacg aatgtcacca atccttgaac
     9781 cgtctaacca atcgcgtaca tcggtatata ttggtaaaaa acggctccaa agagcttcca
     9841 taaagaagag gttcttgctg cgtgcaagat caacaagttc gcgacccatc ttttcgttaa
     9901 ttgtcagggg cttttcgcaa agaacagatt ttccggcgtt gaggcacagt ttgacattat
     9961 catagtgaaa gctatgagga gtcgcgatgt acacagcgtc aagacccgga tcatcaagca
    10021 tttcactgta gctgccgtaa gaatttttaa cagaatattt accggcaaaa gcatccgcgc
    10081 gttcttttct gctgcttgcc acagagtgca gaaccgcgtt atcaacaaag gctatacctt
    10141 tcgcgaaaga ctccgcaata cggcccggac cgattattcc ccagttaacc ttttccattt
    10201 atgactcctt aataatatta aatttcgatc cggcaattaa aattaacagg acaaataacc
    10261 accggaggtg cagacggccc tgttaaaaca gatggcactt tttcagtttc agcatactat
    10321 ttattagccg gatcattgat acagcttgat cagctaaaaa ttagcgaaga aacaggatca
    10381 agtcaaggtt atattattta tccgggacag tgaccatgca tcataagcaa atattgggga
    10441 cggtgtaatt tagcgattaa tgccataaat ggggacggag gtattaaaat gctaataggg
    10501 ccggaggtat aatggggaca gtatacttta cagttaacca gaaaaaaggg acggagcact
    10561 ttctcatccg attccgaaaa cagatacagc atgtttgagc taataatatt attttaagga
    10621 acaagctctt atattatgcc gtaaaccggc cttacaataa ttcagtagat ttcagaatat
    10681 tacacgttat gatttttttg tctgttgaca ttacccgtga agctgctgta ttgttagcca
    10741 gctttatttt gagctgaagg caagtaccat gaacaacggg caaaagcacc cacgtagcat
    10801 tacgattcca gatgtcgaag agactgaaaa actgatccta aaactaaaag agctaagctc
    10861 atttattata gacacctctt cacttatcta tatgcagaag atttctgtca tgaaaatggt
    10921 gacgaaaata tataatatca aggcgcccct ttcggttatt aatgaatttg gcagctgccc
    10981 agagggcatt acgacggttg acattaacct tagtgcagat gatgatatta ttgcatgtgc
    11041 cgatcagttt gggctacccg tttgcagtga agataagcga gtacttttac acgcaagccg
    11101 cgacaatatt gactattata acacaataat gattcttgtt tcgatgcgta ccaaaaatat
    11161 aattacaaaa caagaattcg ggcagtacat tacgggcctg cgctcaaacg cgcattactc
    11221 taataaagtc tgggattacg cgcagaagct gattgataca gtagatatgg tttaaaacga
    11281 ggacaaaatg cgaagacacg acatagagat tacagatcag gaaatactat tttaaagtca
    11341 cgaaacaact ttcattctgc tttttaaaat tttatataat tccgactttt cgcgaatact
    11401 ggacaattta ttaaagtaat catcaaaaga ttcaggttta agactgttat ataagttttc
    11461 ctgtaaatca tgtttcattt tgacacaatc aaattcttta tctttcatca cttattacct
    11521 ctctgggtgt tctaatttcc agtaaattgt atccttcacg aaggttcact gaattgaatt
    11581 ggtgaatttt tttcaagttt actatatgct taaaattcca gcttaccagt acatcaactt
    11641 tgcaaattgt cgctattgca atatgcaaag catcatctga atatttcgga gaaacaattt
    11701 ctgaatcaag ataaaaagcc gctaattttt tcatttcatt atctgcttca attaactcta
    11761 aatcgtcaaa atcaagtaaa agatttttta cccgatcggg agcgttttca agttcagcga
    11821 ttgtaatatc agaaataaca ggcaaatatt cgtttttccg aaatttattg aacaatagta
    11881 atgaatactc agaaaactct tgatcaaata caccgccaaa aacagaagta tctatatata
    11941 ttttgaatat tttcataatg aaaatataaa gcatgtaatt agaaatacaa caacaaatta
    12001 tcagtttagc attataaata tttaacagga taccttaagt aattatcatg ataacgagga
    12061 caaaatgcga agacacgaca aagagattac agatcaggac accataaagc agattctaaa
    12121 ggagaacact gtttgccgta tagctctctg tgataacgat acaccctata tcgttccgat
    12181 gaactacggc tttgatgaaa taaatatata tctgcattgc gcgaaagagg gcaaaaagct
    12241 tgatattatt aaagaaaaca gccgggtatg cttcgaagta accgattcgg ccgaaacagt
    12301 cacatcggat aaggcctgcg gttacggtac aagataccgc tgtgtaattg gacaaggccg
    12361 cattgcactt gtagattcag cagaagagaa gatacaggca ctaaatatta taatgaaaca
    12421 gcacaccggc aaagccgatt gggattatga cccgtcggtt gtagagaaga ttacagtttt
    12481 aaaaattacg gccgaatcaa ttaccggaaa aatatcgggt ttttaagaat aaagattgga
    12541 tcaatcaaag tcgataattc actatagaac ttaaaaatcg atttaattgt aactaataga
    12601 cttttagctt taacaatcat tttttattat taacacctga cataaatatt taaaaaaaaa
    12661 gactattatt tgtttatatt
//
LOCUS       DAOTGZ010000063        12498 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_3064
            NODE_6023_length_12498_cov_4.625332, whole genome shotgun sequence.
ACCESSION   DAOTGZ010000063 DAOTGZ010000000
VERSION     DAOTGZ010000063.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868088
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 12498)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 12498)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:22:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,785
            CDSs (total)                      :: 2,751
            Genes (coding)                    :: 2,732
            CDSs (with protein)               :: 2,732
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 12 of 19
            Pseudo Genes (internal stop)      :: 5 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12498
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6023_length_12498_cov_4.625332"
                     /isolate="AMR_MDS_3064"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(123..2198)
                     /locus_tag="P5123_05860"
     CDS             complement(123..2198)
                     /locus_tag="P5123_05860"
                     /inference="COORDINATES: protein motif:HMM:NF017021.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AsmA family protein"
                     /protein_id="HRX15818.1"
                     /translation="MKPIKILKAFFLVKISALLILLFLITFVLLFLFFFPEEKVISII
                     NQNAENVLQRSIEIDSISYSLRGVKLTGFKIIDPETETPLFTAKSVQITFALAPLFRR
                     QLQLQDLYVEELNFSLDYSNGRYNIENLIEDIKLGSTKSGGNFTSRLPDIHLKECTIE
                     VANTSDFLAPLKGTYIIDGSIFLENGSKPVFDSFIITLPVQRGKIETDLTANLEGDSF
                     KLTGKANLDDVNLSWVYLFGAPNPPQLPYRVTSGTIENLVITSEYTEGDIGKLRCTLN
                     TGDLLQVKDGHCRVYHSPKVLVLDQNNGQIGNSGSFFVQNLTVGITEGYSSFNITAED
                     TALEKISVMIPPIEDMPLHGKLNGKLQYDGKLFNGTVKISNGGYGKTLQGVNGSFNIT
                     NNRFRHENINVLIMDQPAVISAASTSSSFDNFAINVVTDKFIIDTESETGLNLPFLKN
                     TNINGVIEAKEIVVNDIILNNTSIGYSLGNSIIIIQRFKTNFFNGLLTGRAQINQATN
                     GIKTELDINFSNIQLQNLHSWADQIKNRFFGIASGRVNLVVMNSNSKASVKEGNLVIR
                     INNGKLINTGIQDSFGIILKPLRYKLKDLEFKEMFANIEIDNNGYSFNTFMFNSPDIR
                     ASVNGMINHNNVANLNITLLFNTNFIQDVPNFAYPQFSKYKKGSWYIIPLKINNKDIT
                     ENTEVTISE"
     gene            complement(2176..3396)
                     /locus_tag="P5123_05865"
     CDS             complement(2176..3396)
                     /locus_tag="P5123_05865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003006165.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic nucleotide-binding domain-containing
                     protein"
                     /protein_id="HRX15819.1"
                     /translation="MAQGHFKVENYHPNSFIIVEGKQSAGTFFIILKGRVKINNQNPS
                     IADRNNKILGPGDFFGVISCMSTHARIETAVSLEMVSVIVVRREQFGELIQRNPDIAM
                     KIIRFFSRQLRFFDNSITKLTLKDQAEENPEHLYQIGEYYLVKRAFNHAAYAFQRYLQ
                     YCPAGKNSERSLHRLKQLKAPFKAPEPPPTKGLTRTYKDNTMIFCENEFGFELFIIQS
                     GQVKITKVVDEEILLAMLKPGDVFGEMALLDNRPRSASAITFGDVTVMVINKDNFETM
                     VISQPQLATRLIVLLSERIWTAKRQLENLTINDQIGRAYDILLIQLEKQKIQIRPKQP
                     HNFQFGVNELTNMIGATKKQADALMVQLFAEKIITIDEGKLICNDIFELEKTARFYLK
                     KSALERKREANKNS"
     gene            3722..4246
                     /locus_tag="P5123_05870"
     CDS             3722..4246
                     /locus_tag="P5123_05870"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRX15820.1"
                     /translation="MKRLLSDSVLPFVNQGRINMHKVNMLIELKEFVDRVATKGYIGE
                     KTVASLLDRFGVEPEVITWGDYFQSELVFDAVRYSDEELRKVVDTIKFDIMSGYQIFC
                     EHSADFFEWVEKTSNEIILNSTESYSEEEQEILHLKILKDYYCNIGIVDKFSPEEVLW
                     YAGFEENRANAEVS"
     gene            4250..4921
                     /locus_tag="P5123_05875"
     CDS             4250..4921
                     /locus_tag="P5123_05875"
                     /inference="COORDINATES: protein motif:HMM:NF014626.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003697 - single-stranded DNA binding
                     [Evidence IEA];
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_process: GO:0009432 - SOS response [Evidence IEA];
                     GO_process: GO:0018142 - protein-DNA covalent
                     cross-linking [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SOS response-associated peptidase"
                     /protein_id="HRX15821.1"
                     /translation="MCGRFCFIHTSKVIEERYGISVPSLFNPTYNAAPAQILPVISSA
                     DRGKLSFYRWGLELSVPRKDRPAYNNINARAETLEQKKMFKNLIGRKQCIIPVSGFYE
                     WKREGRNKRPFLFTTKDEIYSLAGLWDYIPGDDNMGIYCFTIITRPADEEVSKLHDRM
                     PLILRPDEEKSWLECEINSKNLLNLGKNIKLESVEVSPEVNKVANNHPGLIQPYSSPE
                     NPTLF"
     gene            complement(4936..5340)
                     /locus_tag="P5123_05880"
     CDS             complement(4936..5340)
                     /locus_tag="P5123_05880"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009162168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016790 - thiolester hydrolase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA thioesterase"
                     /protein_id="HRX15822.1"
                     /translation="MSWIYEKNFEVRDYECDLQGIVNNAVYQNYLEHTRNSFLKEKGP
                     DLKALHDQGIDAVVARIEIDYKFPLRASDNFFCRLAVKRDGELKLIFNQEIVLKDSQK
                     LCARAKVVVVCLENGKVSMPKVIIDSFSEYLE"
     gene            complement(5324..6010)
                     /locus_tag="P5123_05885"
     CDS             complement(5324..6010)
                     /locus_tag="P5123_05885"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF013742.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HRX15823.1"
                     /translation="MPIESITVNEKLLAYITSISLRENHFLEMVRKRTETHPCAKMLI
                     TPEQAQFMTMLIKLIGAKKCLEIGVFTGYSTLATALGLPKDGKIIAIDRSREWTSRAL
                     RHWQEAGVDDKIELRVGEGMVLIPALIDEGHTNTFDFIFIDADKKNYENYYQMAIKLC
                     KPNGLIMVDNTLWRGSVIQKDTEDLSAKQLKSFNKMVFEDTRVDITVLPMADGIHLIR
                     KKGEADELDI"
     gene            complement(6017..6499)
                     /gene="ilvN"
                     /locus_tag="P5123_05890"
     CDS             complement(6017..6499)
                     /gene="ilvN"
                     /locus_tag="P5123_05890"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013684085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:1990610 - acetolactate synthase regulator
                     activity [Evidence IEA];
                     GO_process: GO:0009082 - branched-chain amino acid
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase small subunit"
                     /protein_id="HRX15824.1"
                     /translation="MEQYVLSVKVENRSGVLARVVGLFSARGYNIDSLSVSKTEDSEV
                     SVITIVVHGNDRVIEQVKKQLNKLIDVIKVYDHTSIPAAQREIAIVKIQLTPVKRVEV
                     FQIAQTFGAEIIDISQKSLSIQVVGHPTKIENFIDIIRNYGIKELIRSGRVSFARDRA
                     "
     gene            complement(6499..8172)
                     /gene="ilvB"
                     /locus_tag="P5123_05895"
     CDS             complement(6499..8172)
                     /gene="ilvB"
                     /locus_tag="P5123_05895"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012673975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005948 - acetolactate synthase complex
                     [Evidence IEA];
                     GO_function: GO:0003984 - acetolactate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009097 - isoleucine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009099 - valine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biosynthetic-type acetolactate synthase large
                     subunit"
                     /protein_id="HRX15825.1"
                     /translation="MKGARQVVESLIKENVEIMFGYPGGVLIPIFHELHDSPINFILT
                     RHEQGAIHAADGYARATGKPGICIATSGPGATNLITGLANAHMDSIPLVVITGQVSTD
                     MIGNDAFQEADVTGITRPVTKHNYLVQDIHDLARVIKEAFYIATTGRPGPVLIDIPVN
                     ISNADCEYDYDKPVDIRGYKPVYEGHPMQIKKAAQMIKKSKRPVIYSGGGVILGDASK
                     ELTEFARKTGIPVTTTLLGLGAFPETDELSLEMLGMHGTYYANHTVNESDLLIAVGAR
                     FDDRVTGKISDFIPNAKVIHIDIDPASVSKNVVVDIPIVGDCKHILQELINIVEKPDI
                     APWIQEVKQMKATHPLYYDTESKKLKPQYVVERIYEITKGDAIITTEVGQNQMWAAQF
                     YKFSKPRTFISSGGLGTMGFGLPAAIGAQLGVPDKRVINIAGDGSIQMNIQELIVAVQ
                     HRLPIVIAILNNGFLGMVRQWQQLFYDKRYSQTCIKCAPDFVKLAEAYGALGIRVDKK
                     DDVDEAIEKALSITDRPVIIDFTVEKEENVYPFVPSGKTVKETILRELA"
     gene            complement(8353..8700)
                     /locus_tag="P5123_05900"
     CDS             complement(8353..8700)
                     /locus_tag="P5123_05900"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRX15826.1"
                     /translation="MQPDTFKAMIQNRYANLENMVKALLRRSFETSVTDNLEKYGLEE
                     KNEIIRLYNEMTSDIQNNLKSIYNDFMQKIDNVIQSAVKTSENNYKVACYSNDIFFND
                     NSFGDTESSEPEE"
     gene            9055..9855
                     /locus_tag="P5123_05905"
     CDS             9055..9855
                     /locus_tag="P5123_05905"
                     /inference="COORDINATES: protein motif:HMM:NF039482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(+)/NADH kinase"
                     /protein_id="HRX15827.1"
                     /translation="MRNLKTYIYHNPHKEQAVNFRQQLESCLVEESVSTVDSIEDADF
                     FVVIGGDGTLLNFSKYIIEQPKPVLAVNMGSLGFLTDVRVDEARLAVRSIVEKNYSTE
                     NRRFLEIRTAGSSYYALNDFVIAKCGLHARMVSIVVTSNGEYVNTYRADGVIIATPTG
                     STAYSFSTGGPIVKPGLDAVVITPIAPHNLNARPIVVPGSDIIELYAESEESDLHSMV
                     DGQCSLALGGKERVQVTLSDKYIQLVKAPSRGYYSILRQKLKWGDKLC"
     gene            9990..10721
                     /locus_tag="P5123_05910"
     CDS             9990..10721
                     /locus_tag="P5123_05910"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRX15828.1"
                     /translation="MEIFVCLETIPEPDCVELVNGRFNYEDCPVRLDASSAYALEAAL
                     SLKDEYDIPVKAVLLDSFERKSIYDEVRALGCDSIEWINSTYTFDPFERASIFADALN
                     PGEGDIVLAGYHCETCCTNSIPMLIALNKSLPYHSEIKSISFDSGHIHLESFKNGCDK
                     GLEVETMVVSLRSAKKLRYPTLRDRLACCDEYISPVYKNCREEEPFATVPASEIVKHE
                     NYSGDTDDKAASLVATMDSQGMFNI"
     gene            10718..11677
                     /locus_tag="P5123_05915"
     CDS             10718..11677
                     /locus_tag="P5123_05915"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRX15829.1"
                     /translation="MTVVLAVADDRFRKRPSIYRNFSTHAQIFAKKNRVKSCCIFFSR
                     DRRVPEEILNSFDNVITADNRFLNGQTDPLPLFQSILDSYKPLVIMSLHHDFLVQYMT
                     QSLIRDFSAVYDVCDIRLEDGEQIMYQPLFRFRYYRKKVMRPGMMIFVRACPSDTEAP
                     AQNIAKVDHFAFRFPKEVQQVCFKNIENNYSIVAGRGLIDNKYFTRYIVSIGDKMKGA
                     ALCTESLRGSDDAVPAANMAETCAILETDFSVFAGEEGNYDLLEKIQCQKYSVYINNC
                     SCSPLLLVSDDIIIEETSRFIEIYNKKLRKVSFTPKKINKKLR"
     gene            11719..12429
                     /locus_tag="P5123_05920"
     CDS             11719..12429
                     /locus_tag="P5123_05920"
                     /inference="COORDINATES: protein motif:HMM:NF024824.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HRX15830.1"
                     /translation="MILITSALMAGCSREEFFDLFTLKSVRVVTTGEQTEDIKELMDR
                     AKGHESEINKKIEAGDSLADVYEALGVAFLLRKNWELSIENLEKAISFGNVAHDIHRR
                     LAIAYSNRGRELNNNSDFDKAVYHYKLALEKNPDNHDAMYGLSLVLFFEKDDKKAGIE
                     LIKELTQKKPDYYEARMAYGRMLYEDGNRNGALSIYQSMMEDLNIDSDARTRFENDVM
                     SNINRITRELASGVADDQ"
BASE COUNT         3646 a         2279 c         2521 g         4052 t
ORIGIN      
        1 tataatagaa taaaacaatt atgttagtgt gaacaatcca gtctgtataa ggtggatcat
       61 taaaatgaat acggtggcat attatgaatg cctgtgatga aggtcaagta taatagaaat
      121 gattattcgc tgatggttac ttcagtattt tccgttatat ctttattgtt tattttcaaa
      181 ggaattatat accagctgcc ttttttgtat tttgaaaact gcggataggc gaagtttgga
      241 acgtcctgta tgaagtttgt gttaaaaagc agtgtgatat taaggttggc cacattattg
      301 tgattgatca ttccgtttac agatgctctt atgtcaggag aattaaacat aaatgtatta
      361 aatgagtagc cgttgttatc tatttctata ttagcaaaca tctctttgaa ttccagatct
      421 ttaagtttat aacgtaaagg ctttaatatg atgccgaaac tgtcctgaat accagtgttt
      481 atgagttttc cgttattaat tcttattact aaattccctt ctttaacgga agctttactg
      541 tttgagttca ttactactag gttaactctt ccggatgcaa taccgaaaaa tctatttttt
      601 atttgatctg cccaactgtg aagattctgt aattgaatat tagagaagtt tatatccaat
      661 tccgttttga ttccgttagt ggcttgattt atttgagcac gacctgttaa caagccgtta
      721 aaaaagtttg ttttaaatcg ctgtataatg ataatgctat tacccaagga gtagccaatg
      781 gaagtgttgt ttaaaataat atcgtttacg acaatttctt tagcctcgat aacaccattg
      841 atgtttgtgt tttttagaaa aggtaggtta agacctgttt ctgattcggt atcgatgata
      901 aatttgtcag taactacatt gatggcaaaa ttatcaaacg aagaacttgt agatgcagcg
      961 gaaataaccg ctggctgatc cataatcaaa acattgatat tctcatgtct aaatcggtta
     1021 ttggttatgt taaaagaacc gttaacacct tgaagggttt taccataacc tccgtttgat
     1081 atttttacag ttccgttaaa aagttttccg tcgtattgaa gtttaccgtt aagtttacca
     1141 tgaagtggca tatcttcaat gggggggatc attacggaaa ttttttccaa tgctgtatct
     1201 tcggctgtaa tattaaatga tgagtaacct tctgtaatac ctacagttag gttttgcaca
     1261 aagaaagagc cactgtttcc aatttgcccg ttattttgat ctaaaacaag caccttcggc
     1321 gaatggtaaa cacgacagtg accatctttg acttgtaaca aatcgccggt attaagagta
     1381 catctaagtt taccaatatc accttctgta tattcagaag ttataacaag attctcgatt
     1441 gttccgctgg tcacgcggta tggaagttgc ggcggattgg gagctccaaa gagataaacc
     1501 caactaaggt taacgtcatc cagatttgcc tttccggtga gtttaaacga atcaccttca
     1561 agatttgctg ttaaatcggt ttcgattttg cccctttgca caggtagggt aattataaaa
     1621 gagtcgaata ctggcttaga tccattttcg agaaatatcg aaccatcaat gatataggtt
     1681 cctttgagag gcgcaagaaa atcactggta ttagcaactt caatggtgca ttccttgaga
     1741 tgtatgtcgg gcaatcgaga tgtaaaattt ccaccagatt ttgtgctacc aagtttgatg
     1801 tcttcgatca aattttcaat attatatcta ccattactat aatcgagaga aaaatttagt
     1861 tcttctacat aaaggtcttg aagctgtaac tgtcttcgga aaaggggggc aagagcaaaa
     1921 gtaatctgaa cgcttttagc ggtaaatagt ggtgtttctg tttcaggatc tataatctta
     1981 aaaccggtaa gttttacacc ccgaagactg tagcttatag agtcaatttc gattgatcgt
     2041 tgaagcacat tttctgcgtt ttgatttata atcgagatta ccttctcttc tgggaagaag
     2101 aacaaaaaaa ggagtacaaa ggtaatcaaa aaaaggagta taagaagtgc agatattttt
     2161 accagaaaaa aggctttaag aatttttatt ggcttcacgt ttgcgttcca gagctgattt
     2221 cttgagataa aaacgggcag ttttttccag ttcgaagata tcgttacata ttaatttgcc
     2281 ctcgtcaatg gtaattatct tttcagcaaa aagctgaacc atcaaggcgt cagcctgttt
     2341 ttttgtggca ccaatcatgt ttgtaagctc attgacacca aattggaagt tgtgtggctg
     2401 ctttgggcga atttgaatct tttgtttttc taactggatg agcaaaatgt cataagccct
     2461 tcctatctgg tcattgatgg taagattctc aagctgtcgt ttcgcagtcc atatccgttc
     2521 gctaagcaac actattagtc gagttgcaag ttgtggttgt gatattacca ttgtttcgaa
     2581 attgtccttg ttgattacca taacggtaac atcaccaaaa gtaatagcag acgcacttcg
     2641 aggcctatta tccagtaatg ccatttcacc aaatacatca ccgggtttga gcatggccaa
     2701 aagtatctct tcatcaacaa cctttgttat ctttacctgc ccagattgta tgataaacag
     2761 ttcaaaaccg aattcgtttt cgcaaaaaat catggtgtta tctttgtatg tgcgtgtaag
     2821 tcctttggtc gggggcggtt caggtgcttt aaaaggtgct ttaagctgtt ttaaacggtg
     2881 tagcgagcgt tcagagtttt taccggcagg gcagtattgc aggtagcgtt gaaaagcata
     2941 ggctgcatga ttgaaagccc ttttgaccag ataatattcg cctatttgat aaaggtgttc
     3001 cgggttttct tctgcctgat ccttaagagt cagttttgtt attgaattat caaaaaagcg
     3061 taactgacgc gaaaagaagc ggattatttt catggcaata tcggggttac gttgaataag
     3121 ctcgccaaac tgttccctgc gtaccacaat tacagatacc atttcaagtg acaccgcggt
     3181 ttctatacga gcatgtgtac tcatacatga aataacaccg aaaaaatcac ctggccccaa
     3241 aattttattg tttctgtcgg caattgaagg attctgattg ttgattttaa cgcgcccctt
     3301 aagaattatg aaaaaggttc ccgcgctctg tttgccttca acgatgataa acgaattagg
     3361 gtggtaattc tctactttaa agtgcccttg agccatttgt cctcggtgaa tttttattca
     3421 ggattttgtt taattaagta aaaattgaaa cttacttggt acttgatatt attttgcact
     3481 caattacgtc aatggtattt ttatattgtt cgcttgaaca ttactgatta aaatacatac
     3541 atacattaac agtatcgacc ttgattacaa aaaaataaaa aaagaagtca taaaatttgt
     3601 ctttaagcaa tattttctaa tggaataggg taaagctccg atatttataa aaagacgaac
     3661 tagaactatg tatttttttt catttttagt atctgatatt ttatgaaccg gagcagacaa
     3721 tatgaaacgt ctattaagtg acagtgtttt accctttgta aaccagggac gcataaatat
     3781 gcataaagtg aatatgctta tcgaattaaa agaatttgtt gatcgcgttg ctaccaaggg
     3841 atatattgga gaaaagaccg tagcgtcgtt acttgaccgt tttggcgtgg aaccagaagt
     3901 aattacatgg ggtgattact ttcagagtga gcttgtattt gatgctgtac gctattcgga
     3961 tgaagagtta cgaaaagtgg ttgataccat aaagtttgat attatgagcg gatatcagat
     4021 attttgtgag cacagtgctg atttttttga gtgggtcgaa aaaaccagta acgaaattat
     4081 tttgaatagt actgaaagct attctgaaga agaacaagag attctgcatt taaagatttt
     4141 gaaagactat tattgcaata taggtatagt tgataagttt tcccctgaag aagttctctg
     4201 gtatgcaggg tttgaggaga acagggcaaa cgcggaagtt tcctgataga tgtgtggacg
     4261 tttctgcttt attcatacct ccaaagtaat cgaagagcgg tacggtatat ccgtaccttc
     4321 gctttttaac cccacatata atgccgcacc cgcacagatt ttacctgtta tctcttcagc
     4381 agacagggga aagctatcgt tttatcgttg gggcttagaa ctttctgtac caaggaaaga
     4441 taggccagct tataataaca taaatgcccg tgccgagacc ttggaacaaa aaaaaatgtt
     4501 taaaaatttg attgggagaa aacagtgtat tatacctgtc agcggatttt acgagtggaa
     4561 gcgtgaaggt cgcaataaaa gaccatttct gttcacaaca aaagacgaaa tatattcttt
     4621 ggcagggcta tgggattata tacctggtga tgataatatg ggtatatact gttttacgat
     4681 aataacacga cccgctgatg aggaagtcag caaactacat gatcgtatgc ccttgatttt
     4741 gagacctgat gaggaaaaaa gctggcttga atgtgaaatc aatagtaaaa atttattaaa
     4801 ccttggcaag aatattaaac ttgaatccgt agaggtctct ccggaagtca ataaggttgc
     4861 aaataatcat cctggtctga ttcagcccta ttcatcgcct gaaaacccga cactctttta
     4921 aaatttaata attacttatt cgagatattc actaaacgaa tctataatga ccttcggcat
     4981 acttactttc ccattttcta ggcaaacaac aactactttt gctctggcgc aaagtttttg
     5041 gctatctttt agtactattt cctggttaaa gataagcttg agttcaccgt ctcgttttac
     5101 agccagacga cagaaaaaat tgtcagatgc tcttaacgga aacttgtaat ctatttcgat
     5161 acgtgccaca acagcatcaa ttccctgatc gtgaagagcc ttaagatctg gacctttctc
     5221 tttaagaaac gaattgcggg tatgttcaag atagttttgg tatacagcat tatttacaat
     5281 accctgcagg tcgcattcgt agtctcggac ttcgaaattt ttttcatata tccagctcat
     5341 cagcttctcc ttttttacgt atcagatgta taccgtcggc catgggtaaa actgttatat
     5401 cgacccgagt gtcttcaaaa accatcttgt tgaaagattt gagttgtttg gctgacaggt
     5461 cttccgtatc tttttgtatt accgaaccac gccaaagagt attgtcgacc attataagcc
     5521 cgttaggttt acacagtttt attgccatct gatagtagtt ttcgtaattt tttttatccg
     5581 catcgataaa aataaaatca aaggtgtttg tgtgaccttc atctataagc gcaggaataa
     5641 gaaccattcc ttcgccaaca cgaagttcta tcttatcatc aactccggcc tcttgccagt
     5701 gacgaagcgc tcgagatgtc cactcgcgtg atcggtcaat agctataatc ttgccgtctt
     5761 tcggcagtcc caaggcagtt gcgagtgtcg aataaccggt aaagacgcca atttcgagac
     5821 atttttttgc gccaattagt ttaataagca ttgtcataaa ctgagcctgt tcgggagtaa
     5881 tgagcatttt ggcgcaagga tgagtctccg ttcgtttacg taccatttct aaaaaatggt
     5941 tttcacgaag tgaaatagaa gttatataag ctagaagttt ttcgtttacg gttatacttt
     6001 ctattggcat ctgatgttac gcgcggtcac gagcaaaaga gacacggcca cttcgtataa
     6061 gctctttgat tccgtaattg cggatgatat ctataaagtt ttctattttt gttgggtgac
     6121 caacaacctg tatagagagt gacttttgag aaatatcaat gatttcggca ccaaatgtct
     6181 gagcgatctg aaaaacttca acacgtttca caggtgttag ctgaattttt actattgcga
     6241 tttcgcgctg agctgcggga atacttgtat ggtcataaac cttgataaca tcgattagtt
     6301 tgttcagctg tttttttacc tgttcaatta cacggtcgtt accgtgaacg acaattgtta
     6361 taaccgaaac ttcgctatct tctgttttgc ttacagaaag tgaatctata ttgtagccgc
     6421 gtgccgagaa aagaccaaca actcttgcca gcacacccga tctgttttcg actttaaccg
     6481 acagtacata ttgttccatt aagcaagctc ccttaaaatt gtttctttta ctgttttacc
     6541 cgaaggaaca aagggataaa cgttctcctc tttttccacg gtaaaatcaa ttattacagg
     6601 acgatccgtt atagagaggg ctttctctat ggcttcatca acatcatctt ttttatctac
     6661 acgtatacct aatgcgccat aagcttctgc cagtttaaca aagtcaggag cacactttat
     6721 acaggtttgt gaatatcttt tgtcgtaaaa tagttgctgc cattgacgta ccatccctaa
     6781 aaatccatta ttaagaatag ctataacaat tggcagtctg tgctgaacag caactataag
     6841 ctcttgtatg ttcatttgaa tggaaccatc accagcgatg tttatgaccc gtttatcagg
     6901 aactccaagt tgcgcgccta tggcagccgg caatccgaag cccatagtac ccagaccacc
     6961 ggaagatata aatgttctag gtttgctgaa tttataaaac tgcgctgccc acatctgatt
     7021 ttgcccaacc tcggtcgtga ttatcgcgtc accctttgtg atttcgtaga tacgctctac
     7081 tacgtattgc ggtttaagct ttttgctttc agtatcatag tagaggggat gtgttgcttt
     7141 catctgtttg acttcctgaa tccaaggagc gatatccggt ttctcaacga tatttataag
     7201 ttcttgtaga atatgtttac aatctccaac tattgggata tctacaacca catttttcga
     7261 aacagaagca ggatctatgt ctatatgtat taccttggca tttggaataa aatctgatat
     7321 tttaccggtt acccgatcgt caaatcttgc tccgacggca ataaggagat ccgattcatt
     7381 tacagtatga ttagcgtaat atgtaccgtg cattccgagc atctcaagtg atagctcgtc
     7441 ggtttctgga aaagctccca agcctaaaag agtagtcgtt acaggtattc cggttttgcg
     7501 tgcaaattca gtaagttctt tggaagcgtc tccaagaata actccaccac cagaatagat
     7561 aacagggcgt ttagattttt taatcatctg tgcggctttt ttgatttgca tcggatgacc
     7621 ttcgtaaaca ggtttgtatc ctcggatgtc cacaggttta tcgtagtcat attcgcaatc
     7681 tgcattagag atgtttacag gtatatcgat tagtactggg ccgggacgac ctgtggtcgc
     7741 gatatagaat gcttccttta ttacgcgtgc aagatcatgg atatcctgta caaggtagtt
     7801 gtgttttgtg actgggcgtg taattcctgt aacatcggct tcctggaaag cgtcgtttcc
     7861 tatcatgtct gtggagacct gaccggttat tacaacgagc ggaatcgaat ccatatgagc
     7921 atttgccagt cccgttataa ggttagtagc acctggtccg gatgttgcta tgcatattcc
     7981 cggtttgcct gtagctcttg cgtaaccgtc agcagcgtgt attgctccct gttcgtgacg
     8041 tgtcaatatg aagtttatcg ggctgtcatg aagttcatga aaaataggta tcagaacacc
     8101 ccctgggtat ccgaacataa tctccacatt ctctttgatc agactttcta cgacctggcg
     8161 cgcacctttc atgcagctat ccctcattta agtagttttt aacaaataag taggtaatta
     8221 tcaagataaa taggtaccca tacggtaagg gtatgtttca aataagcatt tacaacacga
     8281 aaggtaaact tattaaagca gtcgaaatag tgtcaaatta ttttttttat gagtgagaat
     8341 agggagattt ccctattctt ctggctccga agactctgtg tctccaaaac tattatcgtt
     8401 gaagaaaata tcattcgagt agcaggcgac tttgtagttg ttttcagagg tttttacagc
     8461 gctttgtatt acattatcga ttttttgcat gaaatcgttg tatatggatt tcaggttgtt
     8521 ctgtatgtcg cttgtcatct cgttgtacag tcttattatc tcgttttttt cttcgagacc
     8581 atacttttca agattatcag tgaccgaagt ttcgaaacta cgacgcaata atgctttgac
     8641 catattctca agatttgcat aacgattttg tatcattgct ttaaatgtat ctggttgcat
     8701 gattatctcc tagtaactga taacgactta aggttattga atctgcttga ttcatgaaat
     8761 taaaaataac cagagtctgc aaaagctgtt ttatcagttt ataaatttaa cagtcacagt
     8821 tcatggatat agacatctac tgctacaata gggttgcaaa aaaatcttaa acctggaaaa
     8881 catttcagtt gtaatattta tcttcttgaa ataacgagta gcttttcatg agatgtatat
     8941 tgtgtttatt taaatccatt tgtaatcttt tgtaatctgt ttcaaaaaac ctttgacata
     9001 taaaaaggca aatgttatgt ctcataaaaa caccttaaca ggaacctcat gctaatgcgg
     9061 aatctaaaaa catacattta ccacaatccg cataaggagc aggctgtcaa ttttaggcag
     9121 cagcttgagt cgtgtcttgt tgaagagtcg gtttcgactg ttgattcgat agaggatgcc
     9181 gatttttttg ttgtgattgg tggtgatgga acactattaa acttttcaaa atatattatt
     9241 gaacaaccta aaccggttct tgccgttaat atggggagtc tgggtttttt aacagatgtc
     9301 agggttgatg aggccaggct tgcagttcga agtatagtag aaaaaaacta tagcacagaa
     9361 aatagacgtt ttctcgagat aaggacggct ggttcaagct attatgctct taatgatttt
     9421 gttatagcca agtgtggact tcacgcaagg atggtcagca tagtggttac ttcgaatggg
     9481 gagtatgtaa acacttacag ggcagacggt gtgataatag ctactcctac gggatccaca
     9541 gcctattcct tttctacagg cgggccaatt gtcaaaccgg gactagatgc tgtagttata
     9601 actcctattg cgcctcataa tttgaatgcg cgcccgattg ttgttcctgg aagtgatatt
     9661 atagagcttt atgctgaaag tgaagagagt gacttacata gtatggttga tggacaatgt
     9721 tcccttgcgc ttgggggcaa agagagggtt caggttacac tctcggataa atatatacaa
     9781 ctggttaagg cgccttctcg tggatattac tctattttgc gtcaaaagtt aaaatggggg
     9841 gacaaactct gctagattca ataaaacaag agagttttgc accgttcgtg atctaaatga
     9901 aattttttta aaatttatca atattaaact tgtaaagaaa acatgccgaa aatatatatg
     9961 acacgcttgt gtaaataagg attaatgcta tggaaatttt tgtttgtctt gaaaccatac
    10021 ctgagcctga ttgtgtcgaa ctagtaaatg gtagatttaa ttacgaggat tgccctgttc
    10081 gtctggatgc gagttcggct tatgctctcg aagccgcact tagtcttaag gacgaatacg
    10141 atataccggt taaagcggtt ttacttgact catttgagcg taaatctatt tatgatgagg
    10201 ttagagctct tgggtgtgac agcattgaat ggataaattc tacctataca tttgatcctt
    10261 ttgaacgcgc ctccatcttt gccgatgccc ttaatccagg agagggcgat atagtgcttg
    10321 ctggttatca ttgcgaaaca tgttgtacaa acagtattcc aatgcttata gctttgaaca
    10381 aatctcttcc ttatcattcc gaaataaaat ccatctcgtt cgacagcggt catatccatc
    10441 ttgaatcatt taaaaacggt tgtgataagg gattagaagt agagactatg gttgtttcgt
    10501 tgcgttcggc aaaaaaactt cgttatccta cacttcgtga tcgtcttgcc tgctgcgatg
    10561 agtatattag ccctgtttat aaaaattgcc gtgaagaaga gccatttgct actgtaccag
    10621 cttctgaaat tgtcaaacac gagaactatt ccggtgatac cgacgataaa gcagcttcac
    10681 ttgtggctac catggattcg cagggtatgt tcaatatatg accgttgttc ttgctgtagc
    10741 tgatgacaga tttagaaaac gtccttctat ttaccgtaat ttttcgactc atgcacagat
    10801 tttcgcgaaa aagaataggg taaagtcgtg ttgcattttt ttttctcgcg accggcgtgt
    10861 accagaagaa attcttaact cttttgataa tgttattact gctgataacc gttttttaaa
    10921 cggtcagact gaccccttgc cattatttca atctattctt gattcttata aacccctggt
    10981 aataatgagt cttcatcacg attttctggt gcaatacatg acacagtccc taatacgcga
    11041 tttctcagcc gtgtatgatg tttgtgatat acgcttggaa gatggcgaac agataatgta
    11101 ccagcctctt ttccgattca gatattaccg caaaaaagta atgcgtccgg gaatgatgat
    11161 atttgtcagg gcatgtccct ctgataccga ggctcctgca caaaatatcg caaaagtaga
    11221 tcactttgct ttccgttttc ccaaagaggt tcaacaggtt tgctttaaaa atatcgagaa
    11281 taactattct attgttgcgg gcaggggact aatcgacaac aagtatttta cccgttatat
    11341 tgtatctatt ggtgacaaaa tgaaaggagc cgcgctatgt acagagtctt tgcggggatc
    11401 cgatgatgcg gttcctgcag caaacatggc agagacttgc gcgattttag agacagattt
    11461 ttctgtgttt gcaggtgaag agggtaatta tgaccttttg gagaagatac agtgtcaaaa
    11521 atattcagta tatattaata attgtagctg ttcacctctt ttattggtat cggatgacat
    11581 tattattgaa gagacaagcc gttttataga gatttataac aaaaaactta gaaaagttag
    11641 ttttacacca aaaaaaatta acaaaaaact tcgttaagtg agtattagaa tatgaacttt
    11701 aaaaaaaagg cggtaatcat gattctaatt acttcagcac ttatggctgg atgcagccgt
    11761 gaagagtttt ttgatctttt tacattaaaa tccgtgcgtg ttgtcacaac tggtgaacag
    11821 accgaagata tcaaagagct tatggatcgt gcgaaagggc acgaatccga aattaacaag
    11881 aagattgagg ctggtgattc tctggcagat gtttatgagg ctctcggtgt ggcgtttctg
    11941 ttacgaaaaa actgggagtt gtcgatagaa aatctggaaa aagctatctc ttttggtaat
    12001 gttgctcacg atattcatag acgtcttgcc attgcatatt ctaaccgtgg gcgtgaactt
    12061 aacaataatt ctgattttga taaagccgtg taccattata aacttgcact tgagaaaaat
    12121 ccggataacc atgatgctat gtacgggtta tcccttgttc tgttttttga gaaagatgac
    12181 aaaaaggccg gaattgaatt gataaaagaa ctaacccaaa aaaaacctga ttattatgaa
    12241 gccagaatgg cttacggtcg aatgctttat gaagatggta acagaaatgg ggcactttct
    12301 atatatcagt caatgatgga agatctaaat attgattccg atgcacgaac acgtttcgaa
    12361 aatgacgtta tgtccaatat taatcgtatt acccgcgagc ttgcctcggg tgtagcagat
    12421 gaccaatagc ggttcgtatc atctgctgct tctttccctg ctttctgctc ctgtttaccg
    12481 tgaaactctg cttcgatt
//
LOCUS       DAOTGZ010000064        12362 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_3064
            NODE_6096_length_12362_cov_5.209799, whole genome shotgun sequence.
ACCESSION   DAOTGZ010000064 DAOTGZ010000000
VERSION     DAOTGZ010000064.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868088
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 12362)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 12362)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:22:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,785
            CDSs (total)                      :: 2,751
            Genes (coding)                    :: 2,732
            CDSs (with protein)               :: 2,732
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 12 of 19
            Pseudo Genes (internal stop)      :: 5 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12362
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6096_length_12362_cov_5.209799"
                     /isolate="AMR_MDS_3064"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..292
                     /locus_tag="P5123_05925"
     CDS             <1..292
                     /locus_tag="P5123_05925"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRX15831.1"
                     /translation="DDTILFRSMKKNDKSKQQFMVYDKASNVITNKIDIYPSGNQTHL
                     NPSVMSGLGVMAYMNNAYWWLHEDSKGNINVVRMNEENFEQKLIPLGEWQLH"
     gene            complement(433..2115)
                     /locus_tag="P5123_05930"
     CDS             complement(433..2115)
                     /locus_tag="P5123_05930"
                     /inference="COORDINATES: protein motif:HMM:NF018884.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016791 - phosphatase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SpoIIE family protein phosphatase"
                     /protein_id="HRX15832.1"
                     /translation="MKPVNTYLKSTKSSVLHILIVEDDKSVGSMLLEMLSNQYICEHV
                     TDIASFRNRFNSFRPDIVLLDLNLPDGSGLDLCREIRKGAIHNDAVIIVMTGDSGEKT
                     VNSAYKAGATDYIRKPFFPTELLAKIKILSENVLSKQNLKQLYDELKLFNKKLYKLTS
                     IINSNINELTKTDIITSIEDVINILDCPYCEIVLFSQQANNPFQINRSPRINFLSFKK
                     LVKLKPLIKDSQENLITFKVKEPNYTVYVYIYKILFNSRKSGVVSLQSTHPLPQQTVD
                     MMALYLDFINLKGADIDIKNRLQNEIDKERKEIHKVRSIQVSLLPDFKEIPGYSIAST
                     FIPMDEISGDFFDAFYISKGVYQIVVCDVCGHGMASSYIGSAIRSIIRSISSQLAEPA
                     RITDTLNRVLIQTSRDIYYFATMFLWQIDFTSNSITYVSTGHPTAFFYKSKSKKIIEL
                     GQTGPLIGLFDDAIFEQKTIAFDTDDTLIVYTDGLIEAPSPIKKELYGTDRLKKQFEH
                     AIKHTKPIDILHSVLGSVYSFMDYSAVDDDVTVICMKKSDADTNGDDDSEKL"
     gene            complement(2169..4439)
                     /locus_tag="P5123_05935"
     CDS             complement(2169..4439)
                     /locus_tag="P5123_05935"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRX15833.1"
                     /translation="MIQDNIKIIIKDQNRSRALLAIAGIILSLLFAPLYETYTTFLYK
                     LMYTASLPMSVSEETAYSMHSGHPAPPALLLKAAASARVAGARNVVICASIHNDSPAY
                     ISENLLNQLSSGKPFALDLLTQSSIQNTVINNFNKYGQTTIFAPSLVAENRYIGNNEP
                     YCGNSFLPLFSKSNIYIKTKIENKTLLHISAALYSLQNKDEFDYKTAEPFYYDTFFQK
                     SPLIRIDFNSLANYPSLIEEKNIHLALLADYTDHKEFSDISDGLLQLLNRALSHPEDK
                     TLRDMFLLAHNDTAVLSKQICDKADSINMNDLDPKEKEGITEAINELNRIDNLLKPIE
                     EINGKTIFIEDFAQTGYSHNKSPRFFGKSYTPGEQSAFLYTSMINHTTRQRCGFFAQF
                     VILIVISSLIWFIFYEYPYRLAFKYTLYAFIFFNIVYYIILNLTGFRFDLSYMHVLFG
                     LTAAGLLAEQNIRITWFNRLRKETGLNISPITIEKIASSIKPVKIPESTNGALLYIQI
                     LNLPEVAAILSSKDFTAIINELNDVFRTDCEKNEGVLVSLERGVFCCAYGFGFSGLDP
                     VNEALETALTFDKCVKSANRYKIMLKKDIQFQCSTAIDYAPISSRISSRGDYNILTLY
                     GSILSVVRSMIRISTEYGAPIVITEDALGHIDTLCTWRALDKVKFPFCNITTTLYQIL
                     GGQELHSPLSDFLNIFEEGQKLFYQKQFKEARSYFRKASRLYSGDGPSLYFIKKCAYY
                     LKTDSNLKRTVDIADL"
     gene            4834..8037
                     /locus_tag="P5123_05940"
                     /pseudo
     CDS             4834..8037
                     /locus_tag="P5123_05940"
                     /inference="COORDINATES: protein motif:HMM:NF013069.2"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
     gene            8204..10219
                     /locus_tag="P5123_05945"
     CDS             8204..10219
                     /locus_tag="P5123_05945"
                     /inference="COORDINATES: protein motif:HMM:NF014763.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein"
                     /protein_id="HRX15834.1"
                     /translation="MKNRVKDRVTNMTIRKKMTIAVILMTFFISLGIGLVGFINGTGA
                     LQKQLDQTAPQMAVYGAEIIRKQLDYYIVAMEGIAGRNVFRGMDWNIQKKALEEETAR
                     MQFMGMGIIMPDGEAWYPDGTRAKLGDRDYFKEAISGRTSFSNIIISRVTNSPVMMIA
                     TPIYNYEDEKPAAVLLARLDGAWLSAVTDDIGFGDEGYSYVIDDTGTLLAHSNREYVL
                     GRKNFINEAQIDPAYNKLAEMFKIMISGKSGYSEYPFLGTDRFFGYAPIKDTKWSIAV
                     GAEKKHVFSEISSVRFYMITATLLFIMLGIGFAFILSQSISRPVGRMVEAIKHLSSGD
                     LTQKLVVESNDEIGNMSGIFNDFVEKLRSVILTISQNVSELASGSNQMSATSSSFSDN
                     AQSQAASTEEVTATVEEVSAGVESVARSSKIQFDHLSDFINQMGSLSESINEMSEKLD
                     QTASITTKIATTAKDGNKSLKDMTISMSRITESSAEMTSIVDIINDISEQINLLSLNA
                     AIEAARAGDSGRGFAVVADEISKLAEQTATSIKEIDRFIIDNNEEINRGMGSVRSVNN
                     TIYGITEGVSQVGEMMKELSEFMKNQQKENASVNSSAKMMRGRSEEMKTATEEQKSAI
                     EEIVKSVSEINEYTQANASGAQQMAATLESFSQMSENLKRSVEFFKV"
     gene            complement(10353..11330)
                     /locus_tag="P5123_05950"
     CDS             complement(10353..11330)
                     /locus_tag="P5123_05950"
                     /EC_number="1.4.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020815675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047850 - diaminopimelate dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0009085 - lysine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopimelate dehydrogenase"
                     /protein_id="HRX15835.1"
                     /translation="MSNKIRIGIMGYGNIGKGVESAIKQNSDMTLSAVFSRRDPSSVQ
                     TEAPVYPVDDIASFTEKIDVMILCGGSATDLPDQGPMTASLFNTVDSFDTHARVPEYF
                     EKMNAAAKKGDTLCLYSCGWDPGLFSMMRVLFESMLPQGSETTFWGPGVSQGHSDAIR
                     RIEGVADARQYTLPIDSAIQAARDGNADDLTTRQKHKRHCYVTVKEGADKEAITKTIV
                     NMPNYFSDYDTSVEFMDHETLLKEHGAIPHGGFVIRNGKTGDNNSETMEFSLKLDSNP
                     EFTASVLVACARAVHRMRSNGTRGALSMLEVPLAHFSSTDIADLRAGKI"
     gene            11542..11982
                     /locus_tag="P5123_05955"
     CDS             11542..11982
                     /locus_tag="P5123_05955"
                     /inference="COORDINATES: protein motif:HMM:NF012792.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HRX15836.1"
                     /translation="MEIVDIVSKVVIKECADLYVRVFNSEPWNDRWNAEAAFERLTDF
                     FDTPRFFGVAVWDNGKPIAAIIGNLERYYSGDYYNLKEMFVSPEYQRAGVGKKLLKHV
                     KRKLAKNRIESIILFTSNEFYTVEFYKKSGFIILDNMRMLRCGL"
     gene            12009..>12362
                     /locus_tag="P5123_05960"
     CDS             12009..>12362
                     /locus_tag="P5123_05960"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRX15837.1"
                     /translation="MRIIVIISLSLLSLLSCVNTPCKWIIAVHSETDNAYVKIEDGEK
                     QADFYLNSGDSFERNISDYRSGTVIIIKTSNLSKTYRIGEYDHWFARTHNIKILIKSS
                     AVDFDSGGLGMQQLSE"
BASE COUNT         3754 a         2032 c         2709 g         3867 t
ORIGIN      
        1 ggatgatacc atattattca ggtcaatgaa aaaaaatgat aaaagcaaac agcagtttat
       61 ggtttatgat aaggcatcta atgtcattac taataagatc gatatctatc cctcgggtaa
      121 tcagactcat ttaaacccct cggttatgag tggtttgggt gtaatggcct atatgaataa
      181 tgcttactgg tggctgcatg aagattctaa aggaaatatt aatgtggtac ggatgaatga
      241 agaaaatttc gaacagaagc ttattccact gggcgaatgg caacttcatt gattattagc
      301 cgtgataaaa tctgtcacgg ttaataatct ttctctgcaa acccgaaacg acttcgtttt
      361 tgcagagaag atgttgtttt gtaaaagatc ctttcttagt ttaatcagaa ataaaaatta
      421 aagctttagt gtctacaatt tttcagaatc gtcatcgcca tttgtatcgg catccgattt
      481 cttcatgcag attacggtca cgtcgtcatc aactgcggag tagtccataa acgaatagac
      541 cgagccaaga acggaatgaa gtatatctat tggttttgtg tgtttaatgg catgttcgaa
      601 ctgttttttt aatcggtctg tgccatacag ctctttttta atgggacttg gggcttcaat
      661 aagtccgtca gtgtaaacta tcagagtgtc atctgtatca aaagcaatgg ttttctgttc
      721 aaagatagcg tcatcaaaaa gtccaattaa gggaccggtt tggccaagct cgataatttt
      781 ttttgatttt gatttgtaaa aaaaagcagt cgggtgaccg gttgaaacat aggtaatact
      841 gttactggtg aaatctatct gccataaaaa cattgtggcg aaatagtaga tatcgcgtga
      901 agtctgaatt aaaacccggt taagggtgtc tgtaatacgc gcgggttcgg caagttgtga
      961 cgagatcgat cggataatcg agcgaattgc ggatcctata taagaagagg ccatgccatg
     1021 accgcaaacg tcacatacaa caatttggta aacacctttg cttatataaa aggcatcaaa
     1081 aaaatctccc gaaatttcgt ccatgggtat aaaggtggaa gcaatactgt agcctggaat
     1141 ttccttgaag tcgggtagaa gtgatacctg aatagatcgc actttatgta tctctttacg
     1201 ttctttgtct atttcgtttt gaaggcgatt tttgatgtca atatctgcac ctttaaggtt
     1261 tatgaaatca agatagagag ccatcatgtc aacagtttgc tgtggtaacg ggtgtgttga
     1321 ctgcaggctt acaacaccgc ttttacggct gttaaacaga atcttataga tatatacata
     1381 tactgtgtaa ttaggttctt taactttaaa agttatcaaa ttttcctgtg aatccttgat
     1441 gagcggtttt aacttaacga gcttcttaaa gcttaaaaag tttattctgg gagaacggtt
     1501 tatctgaaaa ggattgttgg cttgttgaga aaaaagaact atttcgcaat agggacagtc
     1561 aagaatattg ataacatctt caatggatgt tataatatct gttttggtca actcattgat
     1621 gttactgtta ataatagaag taagtttata cagtttttta ttaaatagtt ttaattcgtc
     1681 atagagctgt ttaaggttct gtttggataa tacattttcg gagagtattt taatttttgc
     1741 cagaagctct gttgggaaaa aaggcttgcg aatataatcg gttgctccgg ctttatacgc
     1801 gctgtttact gtcttttcgc cggaatctcc ggtcattaca attattaccg cgtcattgtg
     1861 aatagctcct tttcttatct cacggcaaag atcaagaccg cttccgtcgg gaagatttaa
     1921 atctagcaaa acgatatctg gtctaaatga attgaaacga tttctaaagg aggcaatatc
     1981 cgttacatgc tcacaaatat attgattgct gagcatctcc agtagcatac tgcctacgga
     2041 tttgtcatcc tcgacaatca gtatgtgcaa cactgatgat ttagtacttt ttaaataagt
     2101 attaaccggt ttcattatac caaattacag atttaataat ggtgtttcaa gcaataatat
     2161 ggattttact ataaatccgc aatatctact gttcttttta aattggaatc agttttaagg
     2221 tagtaagcgc attttttgat aaaatagagg gatgggccat caccactgta aagccgggat
     2281 gcttttctaa aataactacg agcctctttg aactgttttt gataaaaaag tttttgacct
     2341 tcttcgaaga tatttaaaaa atctgataaa ggggagtgaa gctcttggcc tccaagaatc
     2401 tgataaagag ttgttgtgat attgcaaaat gggaatttga ccttgtctag ggcacgccaa
     2461 gtgcaaagtg tatcaatatg tcctaaagca tcttctgtaa taacaatggg ggctccatat
     2521 tcggtgctga tgcggatcat tgaacgcact acgctcagta tactaccata aagagtgaga
     2581 atattataat cccctcttga tgaaattcgc gaggatattg gtgcatagtc tatggctgtt
     2641 gaacattgaa actggatatc tttttttagc attattttgt atctgtttgc actttttaca
     2701 cacttatcaa aggttagagc agtttcaagt gcctcattta ccggatctaa acctgaaaat
     2761 ccaaaaccgt aggcacaaca aaaaacgcct ctttcaagag agacaaggac tccctcattt
     2821 ttttcacaat ctgttcggaa aacatcgtta agctcattaa taatagcggt aaaatctttt
     2881 gaagaaagta ttgccgctac ttcgggaaga ttaagtatct gtatgtagag cagagctccg
     2941 tttgtgctct ctggtatctt gacaggttta atgctggatg caattttttc aatggttatt
     3001 ggagaaatgt ttaaccctgt ttctttacgc aaacggttaa accaggtaat tcttatgttt
     3061 tgttctgcta atagtcctgc tgctgtgagt ccaaacaaga catgcatata tgataagtca
     3121 aatctgaagc cagttaaatt gagaatgatg taatagacaa tattgaagaa aataaaagca
     3181 tataatgtat acttgaaagc cagacggtaa ggatattcat aaaatataaa ccagattagt
     3241 gaagatataa caataagaat aacgaattga gcaaaaaaac cacacctctg ccttgtcgtg
     3301 tggtttatca ttgaagtgta caaaaacgcg ctctgttctc caggagtata gcttttacca
     3361 aagaatcggg gcgatttgtt gtgcgagtaa ccggtttgtg caaaatcttc aataaaaatg
     3421 gttttcccat ttatttcttc tatcggttta agcagattat cgattctatt aagctcatta
     3481 attgcctcag ttataccctc tttttcttta gggtccaaat cgttcatgtt tattgaatcg
     3541 gctttatcgc aaatctgttt gcttaaaacg gcagtatcat tatgcgcaag taaaaacatg
     3601 tcacgcaatg ttttgtcttc ggggtgtgat agagctcgat taagaagttg taaaagcccg
     3661 tcagaaatat ctgaaaactc cttgtggtct gtatagtcag ctaaaagagc aagatggatg
     3721 tttttttctt ctattaaact gggatagtta gccagtgaat taaaatcaat acgtattaag
     3781 gggctttttt ggaaaaaagt atcataatag aaaggttcgg ctgttttata gtcaaattca
     3841 tccttatttt gaagtgaata gagtgccgca gatatatgta acaaggtttt gttttctatt
     3901 tttgttttga tgtagatatt acttttgcta aacagtggta aaaagctatt gccgcaatag
     3961 ggttcattat tgccgatata tcggttttct gctacaagcg atggagcaaa aattgttgtt
     4021 tgaccgtatt tattaaaatt atttataacg gtattttgta ttgacgattg tgttagcaag
     4081 tcaagtgcga aaggtttacc cgaagagagc tggtttaaaa gattttccga aatataagct
     4141 ggagagtcgt tatgtataga ggcacagata acaacattac gcgcaccggc aaccctggca
     4201 gatgcggcag cttttaatag aagcgcaggt ggggccggat gtccactgtg catactgtaa
     4261 gctgtctctt cagaaacaga catgggaaga ctagctgtat acattagttt atacaaaaaa
     4321 gtagtgtacg tttcgtacag gggagcaaaa agcagtgata gaatgattcc ggcaattgca
     4381 agtagcgccc ggcttctgtt ttgatctttt ataatgattt ttatattgtc ctgtatcaag
     4441 atttttaccc gttttacagg ctgatcgggt ttatctgaaa ataagaagtt acctgtaatc
     4501 tgattaatgt aaccggagca atgatgctct ccgaaagatc aatcaatcaa aaagacattt
     4561 ggctgtcaat tatagatttg aagtttgcta ttaataagga gtttaaaata acaaccatgc
     4621 cgttgaataa aattttttaa aaacagcatt tgcatttgag gttgtaattt ttgttgacgc
     4681 gttgaacttt gtgtcaaatg aagtggtata cattatgtat tggtgcatta aataattgaa
     4741 aaaatgccga ttgatgaaac aaatgtgatt taaacaaaaa tattttccat acgtcattat
     4801 ctattttgtc ttctaaagcc aacgggggtt accgtgagaa aacttattga atatcttata
     4861 aaaaacgcgc ttattgttaa tcttgtgtcc gtatcggtta ttatcgcggg acttctgttt
     4921 atgtttaacg caaaccgaga agcgtttcct caagtcgatt tcggctgggt tatgatacag
     4981 acagtctatc cgggggcgac tccggtggat gtcgaaaaac tggtatcaaa accgattgaa
     5041 gatcaattac gtgaggtaaa caatataaaa cagatatcct cctactcttt ggagagtatc
     5101 tcttttattg cctgtgaact tgacccagat ataaaaaaca aggataagac tgtccagctt
     5161 ataaaagacg cggttgcgaa ggttaatgat cttcctgaag aggcggaaga tcccgaagta
     5221 accgagcttt ccatggcaga gatacccgta ttaagggtat caatcgcaaa taaaaacgga
     5281 ataaaaagtg ataatgagga gtttgaatta agacattacg ctaaacgcct tgaggatcag
     5341 cttatgatgc ttgagggcgc tgcggagata aagaagaatg gatatcgtga ccgcgaaatg
     5401 catgttgaag tttctctgga taagcttgat gaatactatg tagctcttaa taccattaca
     5461 ggtgcacttg gcgcgaaaaa cctgaacttt ccgggaggta tagcccagga gaataatgaa
     5521 gatgttatta tccgtactat aggagagttt aatacagctg aagagataag cgacgtggcc
     5581 ataagagcta acgatgttgg tggggttgtt aagatcggcg atgtagcctc tgttcgtgat
     5641 acccttaaaa aagaggatat tattaacaag gttaacggtg ccagaagtat aacaatgact
     5701 ttaattagcc gtgagaacta cgatatcatt actctggttc aaaaagcacg tgagatggtt
     5761 tccagttttg aagaggggtt gcctgataaa tacgagatta caataactga cgacctttca
     5821 aaatatgtgc agcgtcgcct tgatgttctt aaaggaaacg ggcttgtggg tattattctt
     5881 gtcgtgcttt ctctttttat cgctttgggg tggcgtatct ctttggtaac tgccgctgga
     5941 ctccctatcg cgtttgctgg aaccttctgg tttatgggtg tacaggatat aagtataaat
     6001 ttgatgtcca tgttcgctct gattatggtt ctgggcatgc ttgtggatga tgccattata
     6061 gttgccgaaa atatatatcg gcatcttgag gagggtgaac ctttactgga ggctgttgtc
     6121 ggtggaactc acgaggtaat gatacctgtt gccggtacaa tcatgactac ggtagccgcg
     6181 tttgcgcctc ttctttttat gggcgggatt atgggtaagt ttatgtgggc tctacccgct
     6241 tgtgtaacaa tagctttaac gatgtcttgg attgaatcca tgctgatact gccttcacac
     6301 atatatgata tcgaaaaata ttataaaaaa cgggaagagt atttcgagaa aaagaaaacc
     6361 aagcaaaaaa agaaagaggc aaaatccagc cccttccgtc ttcgtaaacg atatacttcg
     6421 cttttagctc tatttttgaa aaacaaactg atatttatgg ttcttttgat agcctttttt
     6481 gtcggttctg taggttttgc aatcaaacag atcgagttta aactttttcc gggtggtggt
     6541 atagaggcct gtgttgtttt aatggaagcg gctcccggca cctcgcttga ggctatggat
     6601 gcgaaggtat aaaaaattga aaaaaacata gcagctttac ctgaaacgtg ggtcgagagt
     6661 ttcgtttcgc gtactggata ttacggacag gacgaggggg atcctgaaag caaactgggt
     6721 agtaaatacg gttcaatact tataaatctt actccgtttc aaaatcggga tatcgaagcg
     6781 gaggagattc ttgaagaact tcgtaaggca agtgaaccgg ttataggaga atttaccaag
     6841 attgagttta aaactgccca aggaggaggc cctccaacaa gtgccgacat aagtgtaagt
     6901 gttagagggg aggattataa aatacttgat gagatatcaa ctcagtatca ggaataccta
     6961 aaaggaatac ccggagttga gaacataagg gataactatg aagcggaaaa acgcgagatg
     7021 cgtatttttg tagaccaggg catggctatg aggacgggca ttagtgtttt tgatgttgcc
     7081 tcaactgtca gaatatgtta tgaaggctcg atttcaacat caatcaaacg ttcagaagag
     7141 gagatcgatg tacgtgtgat acttcctaaa gaatcaagac agacacttga gaagctaaaa
     7201 gatattaagg ttgccaacaa aacaggtgga ttgattccgc tcaaggaggt ggcaaaattt
     7261 gttgagggtg tcggtatctc atcgattacg cgtaaagggt ggcgtcggtc tgtagatatt
     7321 tctgcggata ttgttccgcc aaagccggat gagtcgttat cgttctggca aagaattaaa
     7381 aagtcgttta ctgaaaaacc gctgggttct gttgatgtta accttatgat gatggaaaaa
     7441 ttcaaggata ttgacaaccg ttatcccggt tattctgtta gctatgaggg agagtacgaa
     7501 gataccgagg attctataat aaggcttcga aactcttaca taattgctct gcttgccatt
     7561 tatgttatac tggtcgctct tttccataat ttgcgccagc ctcttattgt tatgtccatc
     7621 ataccgttta cacttactgg ggtaatctgg tcgttcttct ttcatggact tccactatcg
     7681 tttatggcac tgatgggagt agttggtctt ggaggtgttg tcgttaacga ctccattgtg
     7741 tatgtggatt ttattaataa ggcacgtgag cggggagaag atacatatac cgcttcgttg
     7801 gaagcgggat acaaaaggct tcgcccaata ttgctgacaa ccataactac tattgctgga
     7861 cttataccta ctgcttatgg aattgggggc agtgatccat ttcttgtgcc gatggctgtt
     7921 gctatggctt atggcttgat gtttggaacg accattactc tttttgctac tcctgtaatt
     7981 tataatattt tttataaaaa ggcctgtaaa cagggtataa aaagctgtga agagtagtta
     8041 agctttgaaa tatttacaaa ttttgatata taataaaatt tatccataat gatttaagtg
     8101 aattttggaa tctgatgatt caatactggg tttttaagcc gttatgcgta gaacaaacaa
     8161 aagttaagat ttttcaaata atttaatata tcgggtgcat gccatgaaga atagagttaa
     8221 agacagagtc actaatatga caatacgtaa gaaaatgact attgcagtaa tattaatgac
     8281 cttttttatt agtttgggaa taggtcttgt aggttttatt aatggaacag gcgctctcca
     8341 aaaacaactt gatcaaactg ccccacagat ggccgtatac ggagcagaaa taattcgcaa
     8401 acaacttgat tattacatag tggcgatgga aggaatagcc gggcgaaatg tttttcgtgg
     8461 aatggattgg aacattcaaa aaaaggctct tgaagaagag actgctcgga tgcagtttat
     8521 gggaatgggg ataatcatgc ctgatggtga ggcttggtat cctgatggaa ccagggctaa
     8581 gcttggtgac cgtgactatt ttaaagaggc aatctccggg cgcacatctt tttcaaatat
     8641 aattatttcc cgtgtgacaa attcaccggt tatgatgata gcaacaccga tttataacta
     8701 tgaagatgaa aaacctgctg cagttttact tgcacggctt gacggtgcct ggctttcggc
     8761 ggttactgat gatattggat ttggcgatga gggatattct tatgtgattg atgatacagg
     8821 tacactttta gcacatagta atcgagagta tgtgcttggt cgcaaaaatt ttataaacga
     8881 ggcgcaaatt gacccggcgt acaataagtt agctgaaatg tttaaaataa tgataagtgg
     8941 taaaagtgga tatagtgaat atccgttttt gggaaccgat cgtttctttg gatatgctcc
     9001 aataaaagat acaaaatggt ctattgccgt tggtgccgag aagaaacatg ttttttctga
     9061 aatttcatcc gtaagattct atatgattac agctactcta ttgtttatta tgctgggtat
     9121 aggattcgct ttcattttgt cacaatcaat ttctcgtccg gtaggccgaa tggttgaggc
     9181 aataaagcat ctttcatctg gtgatttgac acaaaagctt gtagtcgaga gtaatgatga
     9241 gataggtaat atgtccggaa tatttaatga ttttgtagaa aaacttagat cggtgatctt
     9301 gacaattagt caaaatgtta gcgaacttgc ttcggggtct aatcaaatgt ctgcgacatc
     9361 ttcttcattt tctgataatg ctcaaagtca ggctgcgtca accgaagagg ttaccgctac
     9421 agtagaagag gtttctgctg gagtagaaag tgttgccaga agttctaaaa tccaattcga
     9481 tcatctttct gattttatta atcaaatggg ctctttatct gagagtataa acgaaatgag
     9541 tgaaaaacta gatcagactg catcgataac aacaaaaatt gcaactactg cgaaagatgg
     9601 caacaaatcg ttaaaagaca tgactatatc gatgtcgcga attactgaaa gttcagcgga
     9661 gatgacctca attgtggata ttattaatga tatatcggaa cagattaatc tcctctcctt
     9721 aaatgcggcg attgaggcag cgagagcagg agattccggg cgtggatttg ctgttgttgc
     9781 tgatgagata tcaaaactgg ccgaacaaac cgctacaagc attaaagaga ttgaccgctt
     9841 tattattgat aacaacgaag aaattaatcg tggaatgggc agtgttcgct cggtaaacaa
     9901 tactatttat ggcattaccg agggagtttc tcaagtcggt gaaatgatga aagagttatc
     9961 tgaatttatg aaaaatcagc aaaaggagaa tgcttctgtt aacagttctg ctaagatgat
    10021 gcgtggtcgt tcggaggaga tgaagacggc tacagaagaa caaaaaagcg caattgaaga
    10081 aatcgttaaa tcggtttccg aaataaatga gtatactcag gctaatgctt ccggagcgca
    10141 gcagatggcc gcaacacttg aaagcttttc acaaatgtca gaaaatctta agagatcagt
    10201 ggaatttttt aaggtttaat tcatataagt gttcgaagtt tcctattgca ttggctgatt
    10261 atcctagtca atgcaataga atgttttaaa cattctattg cattgcaaaa ggtaatagcg
    10321 gttaagcaag attaatggca ttttaaatcg tattatattt ttcctgctct aagatccgca
    10381 atatctgtag atgaaaagtg tgccaatggt acttcgagca tggatagcgc gcctcgtgtt
    10441 ccgtttgatc gcatacggtg aacggctctt gcgcaggcga ccagaacaga cgccgtaaat
    10501 tctggattgc tatccagctt tagagaaaat tccatagtct cgctgttgtt atcgccagtt
    10561 ttcccgttac ggataacaaa gccgccgtga ggtatagctc cgtgttcttt tagtaaagtt
    10621 tcatgatcca taaattcaac acttgtatcg tagtcagaaa aatagttggg catatttaca
    10681 atagtttttg ttatagcttc tttgtccgca ccctctttta ctgttacata acagtgacgt
    10741 ttgtgtttct gacgtgtagt caaatcgtct gcattgccgt ctctggcagc ctgaatggcg
    10801 ctgtcaatcg gtagggtgta ttgacgtgca tctgctactc cttcgattct acggattgca
    10861 tcgctgtgcc cttggctaac acctggtccc cagaaggtag tttctgaacc ttgcggaagc
    10921 attgattcga agagaactcg catcatgcta aataatcccg gatcccatcc gcaagaatat
    10981 agacatagtg tgtcaccttt ttttgctgca gcgttcatct tttcaaagta ttcaggtaca
    11041 cgagcatgtg tgtcaaagct gtctacggta ttaaaaaggg atgctgtcat ggggccttgg
    11101 tctggcaagt cggtagcaga accaccacat agaatcataa catcaatttt ttctgtaaaa
    11161 gacgcaatat cgtctacggg ataaactggg gcttctgttt gtacagagga ggggtctcgg
    11221 cggctaaata cagcagaaag tgtcatatcg ctattctgtt ttatagcgga ttccacgcct
    11281 tttcctatgt tgccatagcc cataatccct attctaattt tgttgctcat atgttctcct
    11341 tgtatccgaa aaggcatgat aatactaatt tctgaagcct gtcagaataa attttagtag
    11401 catgttttgg taaaaattat ctttgcagaa agggttgttc tgagccgtgt gttgtccata
    11461 atttttttaa aaaaccgttg agtttatatc tataactata gataagtcta cattgcaaag
    11521 aaatataaag ggtgaaacat tttggaaata gttgatattg tttcgaaagt ggttataaaa
    11581 gaatgcgctg atctttatgt gcgtgtattt aatagtgagc cgtggaatga cagatggaat
    11641 gcagaagcgg catttgagcg attaacagat ttttttgaca cacctcgttt ttttggtgtg
    11701 gctgtgtggg ataatgggaa acccatagct gcaataatcg gaaatttaga gaggtattat
    11761 agtggtgatt actataatct taaagaaatg tttgtttcgc cagaatacca acgcgctggg
    11821 gtgggaaaga agttattaaa acatgtaaag aggaaattgg ctaaaaatag aattgaatcg
    11881 ataatactgt ttacttcgaa tgagttttat acagtagaat tttacaaaaa aagtggattt
    11941 attatattag ataatatgcg tatgttgcgg tgtggtttgt aaaatttaat tgctaatggc
    12001 agggtaaaat gagaataata gttataattt ctttaagtct tttatcgttg ctttcatgtg
    12061 ttaatactcc atgcaaatgg attatagcgg tgcattctga aacagataat gcttatgtga
    12121 aaattgagga cggtgagaaa caagctgatt tttacttaaa cagtggagat agttttgagc
    12181 ggaatatatc agattatcgt agtggaacag ttattataat aaaaaccagt aatttatcta
    12241 aaacttatcg tattggtgaa tatgaccact ggtttgccag aacgcataat ataaaaatat
    12301 taatcaaaag tagtgctgta gattttgatt ctggtggact gggaatgcaa cagttaagcg
    12361 aa
//
LOCUS       DAOTGZ010000065        12246 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_3064
            NODE_6188_length_12246_cov_4.372242, whole genome shotgun sequence.
ACCESSION   DAOTGZ010000065 DAOTGZ010000000
VERSION     DAOTGZ010000065.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868088
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 12246)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 12246)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:22:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,785
            CDSs (total)                      :: 2,751
            Genes (coding)                    :: 2,732
            CDSs (with protein)               :: 2,732
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 12 of 19
            Pseudo Genes (internal stop)      :: 5 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12246
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6188_length_12246_cov_4.372242"
                     /isolate="AMR_MDS_3064"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            198..584
                     /locus_tag="P5123_05965"
     CDS             198..584
                     /locus_tag="P5123_05965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013658397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HRX15838.1"
                     /translation="MKSMISHVAIYVKYLEVSREFYTYYFNGLSNDLYQNTKGFSSYF
                     IQFEQGPSLELMHHIELASREVADKVTGYSHIAFSVGSKEAVKSLTNQIVLAGYSLYS
                     SPRSTGDGYFESCVADPDGNRVEITL"
     gene            complement(651..1109)
                     /locus_tag="P5123_05970"
     CDS             complement(651..1109)
                     /locus_tag="P5123_05970"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRX15839.1"
                     /translation="MNSNEIRTSINVDLKYLEIIENHAEKYGMSVSDVMNIILDMHIK
                     KMSKKPRHINRAMKYQERSKTGEIEYKSTHIRLSFITYSRLRDVMILFRYTLSYLLSL
                     AMEFFDEYIGNKTVKFFPPSIHSVIVEFEDDLKMFSNFWCLPPLEFIPGG"
     gene            complement(1296..1730)
                     /locus_tag="P5123_05975"
     CDS             complement(1296..1730)
                     /locus_tag="P5123_05975"
                     /inference="COORDINATES: protein motif:HMM:NF024212.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family winged helix-turn-helix
                     transcriptional regulator"
                     /protein_id="HRX15840.1"
                     /translation="MEEIENKYIVTKLNKTITLFFEKLSEWEESVARKVNLTPRQCHA
                     ISEIGECGPARMKPISERLGVTTGTMTIMADRLDRLKMVKRQTDTNDKRASLLTLSDK
                     GKAVYEEHTLHHLRLSHEFVTALGEEDAELLIKLIDKMNDVL"
     gene            complement(1730..2653)
                     /gene="dmeF"
                     /locus_tag="P5123_05980"
     CDS             complement(1730..2653)
                     /gene="dmeF"
                     /locus_tag="P5123_05980"
                     /inference="COORDINATES: protein motif:HMM:NF033827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDF family Co(II)/Ni(II) efflux transporter
                     DmeF"
                     /protein_id="HRX15841.1"
                     /translation="MKNHSNCELNTNYRFLQKNRKAEKRTFIVSMITVITMVVEITYG
                     ILTGSMALLADGIHMGTHAFALFITLGAYVLSRKLHNNQAFSFGTGKIGVLGGYTNAL
                     LLGATAFFMVYESAARLINPQEINYDSAIMVAIIGLVVNLVCAVILSGAHSHHSHSHS
                     SDKQIASIKHKDTNLSGAIAHVITDAFTSVLAIGALLLGKHAGYDFLDPAAGFLGAAL
                     ILKWSYHLIKDSGSVLLDFGDYQEELTQIRERLESKDVTIKDIHLWRYSENDRALMIT
                     ISDKKMRGANYFKNKISDLIHIDHTTIEVQK"
     gene            2929..3411
                     /locus_tag="P5123_05985"
     CDS             2929..3411
                     /locus_tag="P5123_05985"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRX15842.1"
                     /translation="MSALKKGLWVLFLLLPIAFYSCEDLLDELEDTIEEDGDGFEGRW
                     SIPGSFTDNYNDLEITAADSSGVTGTLSTRAEVGSEIHSFDGTLSGSVSGDNIDITAS
                     GRVKKEISGTYQYIDNVEYHLMLTLSGDTLSGQMAMTKASGVSVGSPYYYSITYERWD
                     "
     gene            3631..6828
                     /locus_tag="P5123_05990"
     CDS             3631..6828
                     /locus_tag="P5123_05990"
                     /inference="COORDINATES: protein motif:HMM:NF020337.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CotH kinase family protein"
                     /protein_id="HRX15843.1"
                     /translation="MIRTAYKRLPISRIVFFILSIVLFLLLLVLSLNRGGTVVFKDPG
                     LDAAIRSHLGTIEEGISLGQLNRITTLDASSYQIKDLSGIENLPRLEVLNLENNSITD
                     LSPLSKIDSLRELNLRNNGILSLKKIRFSSIIDLPITKLSLRHNVVRSEGAEPRRLED
                     ISLLSSMSSLKELELRDNHISDLSPLVNLKNLEYLDLRQNRISDIVPIANLINLKHLN
                     LRENHIKSLLPIRNLVNLQYLNLHSNKNARDFKALESLSAMQELVMRDVPLADGVRYL
                     SGMNNLYRVNLRGCLIDKPDILVSLMKEGALQDNHAQGIIANIDIRDNPLFDSSSTGL
                     EELKLLWKNIDYRRPKQIESAGITRPVFSHKPGFYTDSFYLSLKAKSTDRIYYTLDGE
                     LPKPDEPGTFLYSAPLQIKDRTDEDNFLSLLHTSYPDRKREPLIGSVFKGTIVRALVK
                     EQNGATGPVVTNSYFVSDDILKRYGTPVISISANRDDLFGYDKGIMVAGKIYHENEKY
                     WKTLNPGAYHHTRPANFVQRGNIRLNVGGLKVERYPGGKVAFKIPVHGIGLDFSYFRA
                     PVPQIVIRGTKNYDGFHYLDRSSTRDMLVVRSRYLPEVLPAGATIECVWEREVSLEFF
                     EADGSIAFSQRLGMRVHGESSRSHFQKTLRLYARSEYDDQNYIDYRLFPEYDQNRYKR
                     FLLRQTTERTGLTDIIAQKIMKSINPDMDIQRHRPAAVFINGEFWGWYNIRDRYDDWF
                     LATKYKVSREDIIAIEGNGNLLYGIPGEQKQFLMLDSYIRKTDLSKNDNYKHLTDLMD
                     VENYMTYMAVGIYLNYQDWGTDKHQLVWRIRKPVKDGPVQLDGRWRWLPIDLDGTILR
                     ENNPSLNHLRDTIEDRYSNTFGMFLPYRYHLHFLLQNDQFKNKFINLMADSMNTVLSP
                     VGAREIVKEIVWSQSRKLVLENEKRWKGEKNLDSWDRQIKKTYAFFKQRPVYMRRHIM
                     DYFGIDGTYSLDIVADITMGHVKINSIDIKKGTPGVKDGSYWNGIYFNGIPVDIEAIP
                     AKGYRFVKWRDMPGGQKITICPKSDMVIRPVFEKM"
     gene            6910..7608
                     /locus_tag="P5123_05995"
     CDS             6910..7608
                     /locus_tag="P5123_05995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008515138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4956 domain-containing protein"
                     /protein_id="HRX15844.1"
                     /translation="MSIMDHIVGLAAKNMFLPGPDIFDIIFSLVLSVVIAIVISQIYK
                     LTHRGMSFELSFMSTLVLLSPIVAVVMLFIRGDLVLSLGLIGSLSIIRFRAPIKDTRD
                     MVFLFWSIAVGLGCGTYNWSVVIISTIFITIVMFTLYFVRYGRSRNSDFILVVNGYNE
                     YNSGDIEKILLGYTDDARVRSHESDDNHWEIIYEMRLKDLHEKTVKKLIDEVKAVNGV
                     AKVSLLAPQLALPV"
     gene            7611..8378
                     /locus_tag="P5123_06000"
     CDS             7611..8378
                     /locus_tag="P5123_06000"
                     /inference="COORDINATES: protein motif:HMM:NF020916.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyphosphate polymerase domain-containing
                     protein"
                     /protein_id="HRX15845.1"
                     /translation="MGIAENKKNHDPTAGAWRFELKYRISYFEYYEIRSALVPYMKKD
                     FFTLKEPGEKYLVRSLYYDTYDYKAYDEKMSGDCDRIKFRLRTYSDTYSDDVKVRAEL
                     KVRRGNAMEKFTAFVKGSDYSYFMQNGLWNVKNPVLDEYARYVYMRELKPQILIQYFR
                     EGFEERARGGLRITFDHNVQSIQSDTLFPEKNVFLRHHMPHTVILEIKCRNERPQWLR
                     NLVRDHGLKIIANSKFTQGIQASRHDLYHPDGVVLVR"
     gene            8466..8699
                     /locus_tag="P5123_06005"
     CDS             8466..8699
                     /locus_tag="P5123_06005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006612482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA];
                     GO_process: GO:0006725 - cellular aromatic compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tautomerase family protein"
                     /protein_id="HRX15846.1"
                     /translation="MPHVIIKMYPGRGEDIKKDLVQQIAKVIQDKTGCDDNSISVSIE
                     EIAAGEWPEKVYRPDIMEKANLLYKKPGYNPFE"
     gene            8718..10313
                     /locus_tag="P5123_06010"
     CDS             8718..10313
                     /locus_tag="P5123_06010"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRX15847.1"
                     /translation="MALILRNISFLFLLFLITVSCDGATEDLETVLNSARTNVHAHGN
                     DIYWKGKKVGKFKDVYGQEDDYEHLLKERGKWILKSGRIYTKENIYDDFSTSIGFVKD
                     IRDNNGNKCYVYFSMIVPVSPISSEIVTHYFVYNPGTGELEEVSKIQNSSNYSPVNFS
                     TEVDKLFVDYEPYFSSNEQNQQTINQQSVSGTNSQWRYLFVESRFNYRSWKMTQENPD
                     NAHSKISTSVEPLMLLQIVGGVAAFDSFFKFDYETDFGYGGGSVETGKEAQEQFGLDE
                     NTSYLLKLAAGTKGVEVNYLIEEFQYGNYDYRFSSDDQDPQRDLLQQRAFQTSRRQIE
                     VLYHPGWRDIPRLGGTAGKTKIIDTYFGYRYMTFKVPKIFYSYVDDKDDDDYNVVTGE
                     STPQFVETRAHVIGMGANNHMSSTSPGFNLLYGIELFGGYAQCKMKKYDYYHENYLTA
                     DEKATITEFNYPFIDFGLSLGFSANHKFGMLKTSLFAICHVNYYYELINKFQRGDTES
                     ETDIVSNQENRFTLITAGANIYF"
     gene            complement(10365..10991)
                     /locus_tag="P5123_06015"
     CDS             complement(10365..10991)
                     /locus_tag="P5123_06015"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRX15848.1"
                     /translation="MKITKLIFLIFFTAIISAIAFTSCEEQTDDKDYSDIDYGGEPSP
                     LDLRVSGDQNITARFEFIFCEYSKTEHGYDIDITLLKDKSSQKNHELLFFIHGAEHPI
                     DTFYSENPDDGSWATAIFTYRGISETVEYASTPKKNLILKINEFSVKEGLSGSFTLED
                     SGALINTDGVFDETIYISPASFTFKCSCIFSYSTEENASCESNPLVRP"
     gene            11245..11808
                     /locus_tag="P5123_06020"
     CDS             11245..11808
                     /locus_tag="P5123_06020"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRX15849.1"
                     /translation="MIYDNEEPYNYIDRFCTPWARGKGRKYSIFMIVFLIYGNYIVIQ
                     EICTKDLFHSEPIYNAFIAMLFLVLFSYHIVLFCILFINIWLFAYEVEIYNDYFICYN
                     AFGKEVNHIFYSDVLMIKKTFPNVANNMKIVTTKCKIQIWGGINRYGECIENIRKRSV
                     NLKLVDYGGLDSSKIWTRNDEKFEGQP"
     gene            11922..12059
                     /locus_tag="P5123_06025"
     CDS             11922..12059
                     /locus_tag="P5123_06025"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRX15850.1"
                     /translation="MERITEEEFAYYRSYPQQTYGKIIGGFFFVLATILFFKVCVWIT
                     V"
BASE COUNT         3786 a         2003 c         2454 g         4003 t
ORIGIN      
        1 ttatgactgt tggaataagt tatattgtaa aaatattact ggtcacctct tttgattttg
       61 ccgctcactc tactccgctt tttccaaaat aagagtcttt aatttcttcg gcttattagg
      121 gtgatgtttg tatttacatg attgccctta acaacttatg ataagatgtt ttaataaaaa
      181 aatcttggga gataaaaatg aaatcgatga tatctcatgt cgcaatatac gtaaaatact
      241 tagaagtttc acgagagttt tatacttatt attttaatgg tctaagtaac gatctttatc
      301 aaaatacaaa aggtttttca agctatttta ttcaatttga gcagggacct tcgcttgagc
      361 tgatgcatca tatcgagctt gctagtcgtg aagtagctga taaggtgaca ggttatagtc
      421 atatagcttt ttccgttgga agcaaagagg ctgtaaaaag tcttaccaat caaattgtgc
      481 tggctggtta tagtctgtat agttctcccc gtagtaccgg ggatggttat tttgaaagtt
      541 gtgtcgctga ccctgatggc aaccgggtag agattactct ttagtgtttt ggaagcgatt
      601 tctgaaaaca ggttgatctg gcttcttctt tcagcctgtt ttcgaaattc ttagccacca
      661 ggaataaatt ctaatggagg aagacaccaa aagtttgaaa acatctttaa atcatcttca
      721 aactcaacaa taacgctgtg aatggaggga ggaaaaaact tcacggtttt attaccgata
      781 tattcatcaa aaaattccat agcaagtgat agcaaataag agagtgtgta gcgaaaaaga
      841 atcatcacat ctcttaatct gctgtaagta atgaaggaaa gccgtatgtg agtggattta
      901 tactcgattt cacctgtttt gcttctttct tggtatttca tggctctatt aatatgccta
      961 ggtttcttag acattttttt gatatgcatg tctaaaataa tattcatcac atctgaaacg
     1021 ctcattccgt atttttcagc atgattttcg ataatttcca agtactttaa gtctacatta
     1081 attgatgtcc tgatttcatt agaattcata attattctcc ttagtggttt tcattatata
     1141 agactgattt ttatgaattc ttttaaaaaa aaataaaaaa ttatgacttt tcgtaattct
     1201 tagccaccaa aatgccattt tttgtataaa ttgagctttt aattcggttt tgtgtttatt
     1261 tttttgtagc gacgaaaaaa aagaatacat gatagtcaga gaacatcgtt cattttatca
     1321 atgagcttaa taagaagttc agcgtcttct tcaccaagag ccgtaacaaa ctcatggctc
     1381 agtctaaggt gatgtagcgt atgttcttca taaacggctt tgcccttatc acttagtgtt
     1441 aagagcgaag ctcttttgtc atttgtatcc gtttgtctct ttaccatttt caggcgatcc
     1501 aaccggtctg ccataatggt catggtgccg gtggtaactc ccaatctttc agaaattggt
     1561 ttcattcgag caggtccgca ctcaccaatt tcagatatag cgtgacattg tcgtggtgtc
     1621 agattcactt ttcgggcaac actctcttcc cattctgata gtttttcgaa aaagagggtt
     1681 attgttttat taagttttgt tacaatgtat ttattttcga tctcttccat tatttctgta
     1741 cctcgatagt tgtatgatca atatgaatca gatccgaaat tttattttta aaatagtttg
     1801 cacctcgcat ttttttgtca gatatggtta tcatcaatgc tctgtcgttc tcggaatagc
     1861 gccaaaggtg gatgtccttg attgtaacat ctttggattc aaggcgttct cttatttgtg
     1921 taagctcctc ttgataatct ccaaaatcca aaagaacaga accagaatct ttaattaagt
     1981 gataagacca tttcagaata agagctgctc ctaaaaaacc agcagccggg tcaagaaaat
     2041 catatcctgc gtgtttgcca agaagaagtg ctccaattgc cagaaccgat gtaaaagcgt
     2101 cagttataac gtgagcaatg gctccactta aatttgtatc cttgtgctta atggatgcaa
     2161 tctgtttatc cgagctgtga ctgtgcgaat gatgagagtg tgcacctgaa agaattacgg
     2221 cacagacaag attgacaact agtccaatta ttgcgaccat gattgccgaa tcataattaa
     2281 tttcctgagg gttaattagt cttgccgcag attcgtaaac cataaaaaaa gcagtagcac
     2341 caagaagcag ggcattcgta taccctccaa gaacgccaat ttttccagta ccaaaagaga
     2401 acgcctggtt gttatgaagt ttccgtgaca gaacataagc gccgagagta ataaaaagtg
     2461 caaaggcatg tgttcccatg tgtatgccgt cggctaatag agccattgat ccggttaaaa
     2521 taccatatgt tatctcgacg accattgtta ttactgtaat catcgaaaca ataaaggttc
     2581 gtttttcagc ttttctgttt ttttgtaaaa atctgtaatt tgtatttagt tcacagtttg
     2641 aatgattttt cattatgcaa cctcgtttat tatcttgata gtcaagataa taaatattgt
     2701 ttgatagtca agataaaaat tatattaatg aatttcttct aaatttacaa aaaaaagcat
     2761 taatgggttg acaaattgaa aaaatatgat aaaaagaaaa gtagaatgaa agatattgct
     2821 tggttaataa ttattgtaat tcttgggttt ttatttaaga gactattttt ttaccaatca
     2881 attccaattg atcgggggtt catataaaat caaaggggag gtattgttat gtctgcatta
     2941 aaaaagggtt tatgggtttt gtttttgttg ttacccattg cattttattc ttgtgaagat
     3001 ctattggatg aacttgaaga cacgattgaa gaagatgggg acggttttga aggacgttgg
     3061 agtattcccg gatcttttac agataattac aacgatctgg agattacggc ggcagactcg
     3121 tctggtgtga caggaacttt atctactcgt gcggaagtgg gatcagaaat ccatagtttt
     3181 gatggtactc ttagcggctc tgtaagcggt gacaatattg acatcacggc ttctggacgt
     3241 gtaaagaaag agatatcagg aacatatcag tatattgata atgttgaata tcaccttatg
     3301 ctaacccttt cgggtgatac cctatctggc cagatggcaa tgaccaaggc ctcaggtgtt
     3361 tctgttggct caccatacta ttacagtatt acatatgagc gttgggatta gaatatcaat
     3421 taattaaggt gttattctgt gggtatctgt tataaacaaa aatagttgac tcaatatgtt
     3481 tatagataag aatcgtgcac accgcttgtg gtgactgatt gcgggctact gctttgcgtg
     3541 gattgttagc cagtttgctt gattacgcga tctgtgaaat tctgccgttt tgtcttttgg
     3601 taatcggcag accgggaatt ggagctacat ttgatacgca cagcctacaa aagactgccg
     3661 attagcagaa tagttttttt tatacttagc attgtacttt ttttgttatt gctagtattg
     3721 tcgttaaatc gtggtggaac tgttgttttt aaagatccgg ggcttgacgc ggctataaga
     3781 tcacatctag gcacaatcga agagggtata tctcttggtc agcttaaccg tataacaact
     3841 cttgacgcat catcttatca gataaaggat ctctcgggta tagaaaatct tccgcgactt
     3901 gaagtactta atcttgaaaa caacagtata accgaccttt cacctctttc aaaaattgat
     3961 agccttaggg agcttaatct tcgtaataat ggtatattgt ctctaaaaaa gatcagattc
     4021 tcatccataa tagatctgcc aattacaaaa ctgagtttgc gccataatgt ggtaagaagt
     4081 gaaggggcag agccgagacg tcttgaagat atctcattac tgtcaagtat gagttcgcta
     4141 aaagagcttg aactgaggga taatcatata agtgatttgt cgccattagt aaatctcaag
     4201 aatcttgaat atctagattt aagacaaaat agaatcagcg acattgttcc gattgccaat
     4261 ctaattaatc ttaaacatct taatctgcga gaaaatcata taaagtcact attgccaatt
     4321 aggaatcttg taaacctgca gtatcttaat cttcactcca ataaaaatgc ccgcgacttt
     4381 aaagcccttg aaagcctttc tgctatgcag gagcttgtta tgcgcgatgt ccctcttgca
     4441 gacggtgtga gatacctttc gggtatgaac aatctttatc gtgttaatct acgcggttgc
     4501 ttaatcgata aaccggatat ccttgtgagt cttatgaagg agggggcttt gcaggataat
     4561 catgcacaag gcattatcgc aaatattgat attcgcgata atccactttt tgacagctct
     4621 tccacaggct tagaagagtt aaaattatta tggaaaaata ttgattatcg ccgtccaaaa
     4681 cagatcgaat ctgcaggtat taccagaccg gttttttcgc acaaacctgg tttttatacc
     4741 gatagttttt atctttctct taaagcaaaa agcacagaca gaatatacta cacattagat
     4801 ggagagcttc ctaagccaga tgaaccaggc acatttctct attcagctcc tctacaaatt
     4861 aaggatcgta ctgatgagga taactttttg tcattgcttc atacttcgta tccagatcga
     4921 aaaagggaac cacttattgg aagtgttttt aaaggaacga tagtacgcgc tttagttaaa
     4981 gaacaaaacg gagcaacagg ccctgttgtt accaactcgt attttgttag cgacgatatt
     5041 ttaaaacgtt atggcacacc tgttatttca ataagtgcca atagagatga tctttttggt
     5101 tatgataaag gaattatggt cgcgggtaag atctatcacg aaaacgagaa atactggaaa
     5161 actttaaatc cgggtgcata tcatcatact agacccgcaa actttgttca acgaggcaat
     5221 attcgtttaa atgtaggagg tctcaaggtt gaacgttatc ccggtggtaa ggttgccttc
     5281 aagattcctg tacatgggat aggtcttgat tttagttatt tcagggcgcc tgtgccgcaa
     5341 attgtaattc gcggcactaa gaattatgac ggctttcact accttgatag gagctctaca
     5401 cgagacatgc ttgttgttcg tagtcgttat ttgccagaag ttttaccagc aggtgctacg
     5461 atagaatgcg tatgggaaag ggaagtaagt cttgaatttt tcgaagccga tggtagtatc
     5521 gccttttctc aaaggttggg tatgcgggtg catggcgagt cttcaaggtc tcatttccag
     5581 aaaacactgc gtctctatgc aagaagtgaa tatgatgatc agaattacat cgattacagg
     5641 ctcttcccag aatatgatca gaaccgctat aaacgatttc ttctgcgcca gacaacagag
     5701 cgtacagggc ttacggatat tattgcgcaa aaaatcatga aaagcataaa tcctgatatg
     5761 gatatacaaa ggcatcgtcc ggcagctgtt tttattaatg gcgaattctg gggttggtac
     5821 aatattcgtg acaggtacga tgactggttt ttagcaacaa aatataaagt atcgcgtgaa
     5881 gatattatcg ctatcgaggg taatggtaat cttttatatg gaattcctgg tgaacagaag
     5941 cagtttctga tgcttgatag ttatatacga aaaacagatt tatcaaaaaa tgataattac
     6001 aagcatctta cagatctcat ggatgttgaa aactatatga cctatatggc tgttggtatc
     6061 tatctcaatt atcaggattg gggaacagac aaacatcagc ttgtttggcg aattagaaaa
     6121 cctgtgaaag atggaccggt tcaacttgac ggaagatggc gctggttgcc gattgacctt
     6181 gacggaacca tccttcgaga gaacaatccc tctttgaatc atctaagaga tacaatagaa
     6241 gatagatatt caaatacttt tggtatgttt ctgccatata gataccacct ccattttctt
     6301 ttgcaaaatg accagtttaa gaacaaattt attaatctta tggccgatag catgaatact
     6361 gttttgagtc ctgttggtgc tcgggaaata gtaaaagaaa ttgtttggtc tcaaagccgt
     6421 aagcttgttt tggaaaatga aaaaagatgg aaaggtgaga agaacctcga ttcatgggac
     6481 aggcagataa aaaaaactta tgcgtttttt aaacagagac ctgtatatat gcgacgtcat
     6541 attatggatt attttggcat tgacggaacg tactcacttg atattgttgc ggatattaca
     6601 atggggcatg taaaaataaa ttcgatagat attaaaaaag gtactcctgg cgttaaggat
     6661 ggatcatatt ggaatggcat atactttaat ggaatacctg ttgatattga ggctatacct
     6721 gctaagggtt acagattcgt taaatggcgt gatatgccag gtggtcaaaa aataaccata
     6781 tgtcctaaat ctgatatggt gatcagacct gtttttgaaa agatgtaaaa tgattatagt
     6841 tgttgtttaa aaaagttaaa agaaccgttg cagttttccg gttttaacaa tatttatggg
     6901 gtgaatcaga tgagtataat ggatcatatt gtcggtcttg cggcaaaaaa catgttttta
     6961 ccggggcctg atatttttga tattattttt tcactggtct tgtcggttgt aatcgcgatt
     7021 gttatttcac agatatataa acttacccat agaggtatga gctttgagct atcgttcatg
     7081 tctacgttgg ttcttttatc gccgatagtc gcggtggtta tgctttttat tcgtggagat
     7141 cttgttcttt cgcttggatt aattggctcc ctttcaataa ttcgtttccg tgctcctatc
     7201 aaggatacaa gggatatggt tttcctcttc tggtccatcg cggttggtct ggggtgcggt
     7261 acctacaact ggagtgttgt aatcatatcc acaattttta tcacaattgt aatgttcact
     7321 ctctattttg ttcgttacgg acgttccaga aattcggact ttatacttgt tgttaatggc
     7381 tacaatgaat ataacagcgg cgatatagag aaaatactct taggttatac agatgacgct
     7441 cgtgtacgct cgcatgaatc agatgataat cattgggaaa taatttatga gatgagactc
     7501 aaggatctgc atgagaagac tgtaaaaaaa cttattgatg aagtcaaggc tgtgaatgga
     7561 gtcgcgaaag tttcacttct tgctcctcag cttgctctac cggtttaatc atgggcattg
     7621 ctgaaaataa aaaaaatcat gacccaactg ccggagcttg gcgctttgaa ctgaagtaca
     7681 gaattagtta ttttgaatat tatgaaatac gatcagctct tgtgccctat atgaaaaaag
     7741 atttttttac attaaaagag cctggtgaaa agtatcttgt aagaagtctc tattatgata
     7801 cctacgacta caaggcttac gatgaaaaga tgagtggaga ctgtgacaga ataaaattca
     7861 ggctaagaac ctattctgac acatacagtg atgacgtaaa agttagggca gagttaaaag
     7921 ttcgtcgcgg taacgcaatg gaaaagttta cagcttttgt gaaaggttcc gattatagtt
     7981 actttatgca aaacggatta tggaatgtaa agaatccggt gttagacgag tacgcacggt
     8041 atgtgtacat gcgggagctt aaaccgcaga tacttattca atattttcgt gagggtttcg
     8101 aagagcgcgc gcgtggcgga ttacgcataa catttgatca taacgttcag agtattcagt
     8161 cagatactct tttccccgaa aaaaacgttt ttttgaggca tcatatgcct catactgtaa
     8221 tccttgaaat aaaatgccga aacgaacgtc cacaatggtt acgcaatctt gtgcgtgatc
     8281 acggcttaaa aataattgct aacagtaaat tcactcaggg catacaggct tctcgtcatg
     8341 atctttatca ccctgatggt gtagtgcttg tgcggtaaac atgcgcattt taaattgctt
     8401 gacctttagt ataaattaat ccatataaaa ctataatttc tatatatctg catagtggag
     8461 gctgtatgcc acatgtcata ataaaaatgt atccgggacg cggtgaagat attaaaaaag
     8521 atctggttca gcaaatcgct aaggttattc aggacaaaac cggatgtgat gataacagta
     8581 tatctgtttc gatcgaagag attgctgctg gcgaatggcc tgaaaaggtg tatcggcctg
     8641 atattatgga aaaggcaaat ttactgtaca agaaacccgg gtataatccg tttgaataat
     8701 tgatctggag tgattatatg gctttaattc taagaaatat ttctttttta tttttactgt
     8761 ttctaataac tgtttcatgc gatggtgcaa ctgaggatct ggaaactgtt ctaaacagcg
     8821 ctcgaacaaa tgtacatgcg catggaaacg atatttactg gaaaggtaag aaggtcggta
     8881 aattcaagga cgtctatgga caagaagatg attatgaaca tctgctaaaa gaaagaggaa
     8941 aatggatatt aaagtcaggc cgaatatata caaaagaaaa tatttatgat gatttcagta
     9001 cctctatcgg ttttgttaaa gatataaggg ataataatgg caataagtgt tatgtttatt
     9061 tcagcatgat tgttcctgta tcccccataa gttcggaaat agtaacacat tattttgttt
     9121 ataatccggg aaccggtgaa cttgaagagg tatcaaaaat tcaaaatagt agcaattatt
     9181 caccagttaa tttttcaact gaggttgata aactttttgt ggattatgaa ccatattttt
     9241 cttctaatga acagaatcag cagactataa accagcaatc tgtttctgga acgaatagtc
     9301 aatggcgtta tttgtttgtc gaaagcagat ttaactatag aagctggaaa atgactcagg
     9361 aaaatccgga taatgcgcat tcaaaaatca gtacttctgt cgaaccgcta atgcttttgc
     9421 aaattgtcgg aggtgttgcg gcgtttgatt ctttttttaa atttgattat gaaacagact
     9481 tcggctatgg tggcggcagc gtcgaaactg gaaaagaggc tcaagagcaa tttggtcttg
     9541 atgaaaacac atcctatctc ttaaaactgg ctgctggtac aaagggcgta gaggtgaatt
     9601 atcttattga agaatttcaa tatggtaatt atgattatcg attttcatct gatgaccagg
     9661 atccgcaacg tgatttgctg cagcagcggg cttttcaaac cagcagaaga caaattgagg
     9721 ttttatacca tccgggttgg cgtgatattc ctagattagg tggaaccgcg ggtaaaacaa
     9781 aaattatcga tacatatttt ggttaccggt atatgacttt taaagtgcct aaaatcttct
     9841 attcatacgt tgatgacaaa gatgacgatg actataatgt tgttacaggt gaatcgactc
     9901 ctcagtttgt cgagactcgt gcgcatgtaa taggcatggg ggcaaataac catatgtctt
     9961 ctacaagtcc gggttttaat ttactttatg gtatagaatt atttgggggg tatgctcaat
    10021 gcaagatgaa gaagtatgat tattatcatg aaaattatct tacagcagat gaaaaagcaa
    10081 caataactga gttcaattat ccatttatcg attttggctt atcactgggg tttagtgcta
    10141 atcataagtt tggcatgtta aaaacatctc ttttcgctat ttgtcatgta aattactatt
    10201 atgaattaat taataaattt caaagagggg atacggaatc cgaaacagat attgtaagta
    10261 atcaggaaaa cagatttact cttattactg caggcgcaaa tatatacttt tgatacgaaa
    10321 taatttgcga tagccgttaa tcgaacaaac ggctatcccg ttatttacgg cctgacaaga
    10381 ggatttgatt cacatgatgc attttcttcc gtactataac tgaaaatgca cgaacattta
    10441 aaagtaaaac ttgcagggct gatatatatt gtctcatcga aaacgccatc ggtgtttatt
    10501 aatgctccgc tatcctccag tgtaaatgaa ccgcttagcc cttcctttac actaaattcg
    10561 ttaattttta atattagatt ttttttcggt gtcgaagcgt attcgacagt ttcactgata
    10621 cccctgtaag taaaaattgc agtggcccaa ctgccatcgt caggattctc actgtaaaat
    10681 gtgtcaatag gatgctcagc gccgtgaata aaaaataaca gttcgtggtt tttctgtgaa
    10741 cttttgtctt ttaatagggt gatatcgata tcgtaaccgt gctctgtttt tgagtattca
    10801 cagaaaataa attcaaatct tgcagtaata ttctgatccc cgctaactct aaggtccaaa
    10861 ggacttggtt cccctccgta atctatgtcg gaatagtctt tgtcgtctgt ctgctcctca
    10921 cagctggtaa atgcaattgc gcttataata gctgtaaaaa atattaaaaa aataagtttt
    10981 gtaattttca ttttctttaa cctgggattg atattttgaa tagaattgaa tataatgttt
    11041 aaaatatcaa caaaatttcc tgataatttt gtataaattt taatctgcat atatttcaac
    11101 agcagaattt taagttcggt aaacgtaccc ggaatcctga aaataaagga actatagaat
    11161 ataaaagccc taaatttaaa aaactattga ttcagataaa gattaaatta attttaaggc
    11221 taattattct ctaaggtggt tttaatgata tacgataatg aagaaccata caactacatc
    11281 gatagatttt gcactccctg ggcgcgtgga aaaggacgta agtatagcat tttcatgatt
    11341 gtgttcttga tttatggcaa ttatattgta atacaagaaa tttgtactaa agacttgttt
    11401 cattcagaac caatatacaa tgcctttata gccatgctat ttttagtatt gttttcttat
    11461 catattgtac ttttttgcat tttgtttatt aatatttggc tgtttgctta tgaagttgaa
    11521 atttataatg actattttat ttgctataat gcatttggta aagaagttaa ccatatattt
    11581 tatagtgatg tcttgatgat taagaaaact tttcctaatg ttgctaacaa catgaaaatt
    11641 gtcacaacaa aatgtaaaat ccagatttgg ggcggaataa acagatatgg tgaatgcatt
    11701 gaaaatatcc ggaaacgttc ggttaatttg aaattagtag attatggtgg gctggatagt
    11761 tctaaaatct ggacaagaaa tgatgaaaaa ttcgaaggtc agccgtgaat ataatttaat
    11821 ggctttgata ttggaataaa tgctttatac gtaatacctt atacttttgg cgctatagta
    11881 tggacaaata atattgataa aaagtaaaat aaggatataa aatggaacgc attacagaag
    11941 aagaatttgc atattatcgt tcatatcctc agcagacgta tggaaagatt attggaggtt
    12001 tttttttcgt attagcaacg atactttttt ttaaagtctg cgtatggatt actgtttgag
    12061 tcaaaaatat ttgtagattg ttgtgatgat gtctcagggg ggatacttgt tttcgcagga
    12121 ttatttatat tttttttaag tatacttatg atggattatc aaatagtact cttttggacg
    12181 gggcactatt ttgtaaaact atttgataat cgtattgtag cttataatat gtggatgcgg
    12241 cctaga
//
LOCUS       DAOTGZ010000066        12229 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_3064
            NODE_6198_length_12229_cov_4.729752, whole genome shotgun sequence.
ACCESSION   DAOTGZ010000066 DAOTGZ010000000
VERSION     DAOTGZ010000066.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868088
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 12229)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 12229)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:22:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,785
            CDSs (total)                      :: 2,751
            Genes (coding)                    :: 2,732
            CDSs (with protein)               :: 2,732
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 12 of 19
            Pseudo Genes (internal stop)      :: 5 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12229
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6198_length_12229_cov_4.729752"
                     /isolate="AMR_MDS_3064"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            2..790
                     /locus_tag="P5123_06030"
     CDS             2..790
                     /locus_tag="P5123_06030"
                     /EC_number="1.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003225220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HRX15851.1"
                     /translation="MGLDFVKQALSAGDKVVATARNSEQIKNAIGINSSLLCERLDIT
                     IPEEADTVIKKAMDVFGRVDILVNNAGISYKGFFEEMTIEQINHQLNVNLTGQLNVTK
                     AVLPIMRKQRSGHVIGISSGAGLSGFAYSSIYAASKFAVEGWLDALRKEVESFGINVT
                     IVNPGFYRTELISEKSMTFASAKYSDYDNAREKQTLWWKSQAGKQPGDPKKLARLLVK
                     IAHLDNPPKRLIAGNDVIKLIESKIEELQNDIQFYKKLDFSLEY"
     gene            839..1759
                     /gene="murB"
                     /locus_tag="P5123_06035"
     CDS             839..1759
                     /gene="murB"
                     /locus_tag="P5123_06035"
                     /EC_number="1.3.1.98"
                     /inference="COORDINATES: protein motif:HMM:NF010480.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008762 - UDP-N-acetylmuramate
                     dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate dehydrogenase"
                     /protein_id="HRX15852.1"
                     /translation="MEANIFENLKSYGKVLKDEPMSSYTSFKTGGNADYLVYPASINK
                     CAELIEYLNKYEYNFTVLGGCTNLVVGDMGIRGVVIIISDLTEEFNEIRVIDDNTVYA
                     GAGVKKSDFINFTAENDFDGMHFMVGIPGCIGGGIIMNAGTYMGTFSSVLKKVYYINN
                     RGQSKSKEIDSTMGQYRHLDLEAECAVVTGADFSLPKSDNKELVIQKINDIIEDRKQK
                     HPWDYPSAGSVFKNPENEASWKLVNDCGLKGYSIGGAQVSEKHTNFIINKGEANSAEI
                     RALVSYIQSQVKIKYGIELHTEIRMIGEFH"
     gene            1756..2568
                     /locus_tag="P5123_06040"
     CDS             1756..2568
                     /locus_tag="P5123_06040"
                     /inference="COORDINATES: protein motif:HMM:TIGR00573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004527 - exonuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exonuclease domain-containing protein"
                     /protein_id="HRX15853.1"
                     /translation="MNSSLYDKTFCALDIETTGVNPFKDKIIEIGIVRFRIGQEQNNT
                     YQSLIDPLIPVSEQSRLIHNISQEELEGKPLYSDIYCSIKEFIEDSILVIQNPQFDLS
                     FIQSENRAAGLPFFSNRSFDTVSLAKKVFKGLKNHKLETVSRHLNCIRNFHRALDDAF
                     CCMDIFLEGVKRTDPGRIFTISDLHNICGFDIQERVYKDVGSRKIRGVNISPDKEYEI
                     IYCDRAGQTTERKILCKKIIKNGSTYFVHAFCYLREQDRFFKFSRIKNATLC"
     gene            complement(2560..2787)
                     /locus_tag="P5123_06045"
     CDS             complement(2560..2787)
                     /locus_tag="P5123_06045"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRX15854.1"
                     /translation="MMSDQLVEENIVLDLSSTHYIHSAFIGFLIFLKNEKEKKSKPFS
                     FISSEWLESLLLRLQLNNFFTSRITNERISA"
     gene            2966..3574
                     /locus_tag="P5123_06050"
     CDS             2966..3574
                     /locus_tag="P5123_06050"
                     /inference="COORDINATES: protein motif:HMM:NF014961.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008705 - methionine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009086 - methionine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="vitamin B12 dependent-methionine synthase
                     activation domain-containing protein"
                     /protein_id="HRX15855.1"
                     /translation="MNHLKNIPLSIPYKEIYARLGYSVHKTSLQKSEKDRIDAIIRDG
                     FSLCNFQGIYKQVKVLSVTQKGFVLESGHEIESVYLKDKLKEYGSMIMMAASAGRIVT
                     ETVTELMKKGDNGRAVIYDATASESADAVLDYLQKHIKMQFARSGMAISRLRYSPGYG
                     DIDLSVQKIIYDILELQDYGIILTEYFQLIPEKSVLAIAGLS"
     gene            3571..5268
                     /locus_tag="P5123_06055"
     CDS             3571..5268
                     /locus_tag="P5123_06055"
                     /inference="COORDINATES: protein motif:HMM:NF014615.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="homocysteine S-methyltransferase family protein"
                     /protein_id="HRX15856.1"
                     /translation="MTREEFAQLCLNKTVILDGSTGIELIRRKMKVSGSIETSILDSP
                     DMLNELQLQYCKAGADIILTPTLGANRVKLQEYSFDGSVRELNRELASLSLKTASSFP
                     TKVFGDISSTGRFIEPFGDLLFDDAVEYFREQAEGLLEAGVNGFFIETMIDLQEARAA
                     LIAVRELCDLPAVVCLSFYKEGYTLNGSSPQSVVVTLQSLGADAVGCNCSTGPAEMIP
                     IIQKMATCTKLPLVVKPNAGLPSLKNGKTVYSMDKNEFLSYIPNFHKLGVSLIGGCCG
                     TDPGFINDIAVQWSNKVPVKRESFITHAIASNRTITRFGAGSFTLIGERINPTGKKDF
                     QKQLREKDLTTIEIFASKQQEFGANVLDVNMGLSGIDEREMMLKTISLLSRISSLPLC
                     IDSTRPEVIESALRLYPGRALVNSISGEKDRLEITLPIAAKYGAMLIILPLDDNGIPV
                     TFEERVLVIRKIIDEATKYGYTTDDMVIDGLVMTISSDQKAAKTTLDLINWCAKNGIN
                     SVCGLSNVSFGLPQRDIVNSAFLALGAQRGLTSAIANPSSERLRNIISSINKKGCRI"
     gene            5265..7409
                     /locus_tag="P5123_06060"
     CDS             5265..7409
                     /locus_tag="P5123_06060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003516591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="homocysteine S-methyltransferase family protein"
                     /protein_id="HRX15857.1"
                     /translation="MNILTKLLEKNEILILDGATGTELAERGMPSGVSPELWVLDNPS
                     SIKSVQNDYFNAGSDIVYSCSFGGNRIKLSQYTEDLSAVFDINRKLAIISKENSHGGI
                     VFGDMAPTGKFIEPFGDLAFSEAVDVYREQAAGLCEGGVDALVIETMMDLQETRAALL
                     GAKQAAGQRPVVVTMTFDADGYTLNGTHILSALSIIQSLGADAFGCNCSTGPAEMLEL
                     IKIISPYSRIPLMAKPNAGLPKLIDGKTVFTMGAEEFASYSKQFIDAGVRIIGGCCGT
                     TPNHIKKVKEALENYSPSAISAKKSFVIGTVKDAQVASVDEPLMLINTEAGDKYRNCS
                     ADELSASLVEFCIHNSYIPVIKYDNEANLSFALNVYPGRALIVVENEQDFQTVLPLAE
                     KFGAVIITDKFAPQNVSNTPCLTDIVVICNVTKNENYLNAYKLSSMGEEDERFFAVSE
                     LYNNEIPVLVGDPTDDLRDFIVAYDALTGRDRKLEGLMNRLHQTDNKQISDQEIEERD
                     PVYMAVLRGRDNVIENAIGDSLKAGISPGKIVDEYLIPAINVVGDLYEKKEYFLPQLI
                     MSADTMRKGFKILEPLLAAENSSKSKDGLIVIATVKGDIHDIGKNIVALMLRNYNFDV
                     IDLGKDIDDKTIVDSAIKNKADIIALSALMTTTMVQMKTVVDTAKKSGCKAKIIIGGA
                     VVDQNYCTEIGADGYSSDAMEAVKLAQRLVSA"
     gene            complement(7417..8157)
                     /gene="rlmB"
                     /locus_tag="P5123_06065"
     CDS             complement(7417..8157)
                     /gene="rlmB"
                     /locus_tag="P5123_06065"
                     /inference="COORDINATES: protein motif:HMM:TIGR00186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008173 - RNA methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009451 - RNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA
                     (guanosine(2251)-2'-O)-methyltransferase RlmB"
                     /protein_id="HRX15858.1"
                     /translation="MENGIVFGRIPVLEYLRSGLATSSTTLLIASGIKENSLTEITSL
                     SKKRGVRSERADKDRLEKLCGGDKNHQGIALINCPLYPEFEENQLEAIASNKGIVLIL
                     DQLTDPHNIGAIIRSAEAFGCKGVIITKDNSASVTSTVIKSSAGASAYMPLMKVTNLA
                     RTIDLLKDKGFWIIGSSDKGQESMSKISEYSPLAIVIGSEGTGMRDLTEKKCDLVVRI
                     ELTGKVSSLNASVAASILLYEASTRIKK"
     gene            complement(8142..9566)
                     /gene="cysS"
                     /locus_tag="P5123_06070"
     CDS             complement(8142..9566)
                     /gene="cysS"
                     /locus_tag="P5123_06070"
                     /EC_number="6.1.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020259281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004817 - cysteine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006423 - cysteinyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine--tRNA ligase"
                     /protein_id="HRX15859.1"
                     /translation="MVKFYNTMSKKIEEFIPGGEKKESINDYPPVSIYSCGPTVYGFA
                     HIGNFRTFLFNDILRRFLKFRGFKVNHTMNITDVDDKTIKGALSEGVSLKEYTARFTD
                     IFFEDLKKLNIEEFEHYPRATDSIDAMNDIINRLEKKGLVYKDNNSIYFSISKYSDYG
                     KLSKIDPESIKSGTRYDTDEYHKDDIRDFALWKEVSKDEPGWQIDLGYGRPGWHIECS
                     AMIRKIYNTTIDIHTGGVDLVFPHHENEIAQSEAAFDEPFVRYWLHAEHLLVDNSKMS
                     KSKGNFYTLNDLIEQGFSSRAIRYLLLTAHYRKQFNFTLDNLKNASQPLSRIDNLVLN
                     CEKSQKSGEVRELIKDRIDKLINDFTSSLDNDLNVSGALGHFFEAVTDFNKYYTQSNF
                     SSEEARLILDCLEKIDTVLGIIFFKEEKKAGLSEQEIEDYIIKRDNAKKDKNWNEADR
                     IRDLLLDKGVVLEDSKEGTRWKTV"
     gene            complement(9749..10255)
                     /locus_tag="P5123_06075"
     CDS             complement(9749..10255)
                     /locus_tag="P5123_06075"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRX15860.1"
                     /translation="MDTTKAIETLTVNETEWEKTLPSHILSDKRIKNFIQASRQNGND
                     NKIYAHVELVSVDGKTDQIIKKLKKKLKNRENILLSITKQMNKALKRKNEKIKILLGY
                     IKLLENLLREHNIDFRIVKKRYKYGEINLASRPAGTNQTSSSQTIVDQYTDTVEIILD
                     NDGNEILS"
     gene            10626..11903
                     /locus_tag="P5123_06080"
     CDS             10626..11903
                     /locus_tag="P5123_06080"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009975 - cyclase activity [Evidence IEA];
                     GO_process: GO:0009966 - regulation of signal transduction
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HRX15861.1"
                     /translation="MYTVLHIDNSHFFNKIVRSCIEKIGLTCITSNDISEAVEKLENN
                     HIDLIITALEIRGGGGEYLIRQLNSGKFRNIPVIVMTSNDSMSTRIKMFKLGVVDYIS
                     KDQTFAGALSGFLNKLTEKNRSRSLLKKLSFAVLDDSKTQLQVISNIMSLNKIENVSF
                     FSKGGDLLKSNNRFHIYFVDLVLPDISGEQAILQIRKKYPESLIIAVSGIDNYKVVSN
                     VLLSGADDYILKPFNSSIFMARLVSNVRVFQLVQDLKKKNQELEESLITDPLTGLYNH
                     RFIYSRLQDIISKKTSPFSFVFFSVDNFNNINDTYGHSQGDKVLCKISDVLLGEIEDR
                     SCIGRYGGVEFIVLFPGMTVDKAFPVAEKIRQRVNRITVNNARISVSGGLCQNEGEER
                     AEDLIRKADSLLFNAKHNGKNRIEKSFDWQSYL"
     gene            complement(11894..>12229)
                     /locus_tag="P5123_06085"
     CDS             complement(11894..>12229)
                     /locus_tag="P5123_06085"
                     /inference="COORDINATES: protein motif:HMM:NF024598.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative glycoside hydrolase"
                     /protein_id="HRX15862.1"
                     /translation="AKLSQYCDIICPMLYPSHFNNNFRGIAKPGDSPYYFISEGVTRV
                     KKQSGKTMVRPWLQAFGWRVSNYDASYIQEQIKASIDTKSEGYIFWNAKNSYDTVIDG
                     MEGLNSLYK"
BASE COUNT         3880 a         2132 c         2371 g         3846 t
ORIGIN      
        1 tatggggcta gattttgtta agcaagcttt gagtgctggt gacaaggtag ttgcaacagc
       61 aagaaactct gagcaaataa aaaacgccat tggtattaat tcatcactac tgtgtgagag
      121 acttgatatt accataccag aagaagctga tacagtaata aaaaaagcaa tggatgtttt
      181 tggaagagta gatattcttg taaacaacgc tggtatttcg tataagggtt tttttgaaga
      241 gatgacaatt gaacagatca atcatcagtt gaatgtaaat ctgactggac aattaaacgt
      301 aacaaaagct gttttgccta ttatgagaaa acagaggtca gggcatgtta tagggatatc
      361 ttccggagcc ggcttgtctg gttttgctta cagctccatt tacgctgctt caaaatttgc
      421 tgtcgagggc tggctagatg ctttaagaaa ggaagttgaa tcttttggca taaacgtcac
      481 tatcgtaaat ccgggttttt acagaacaga actaatatct gaaaaatcaa tgactttcgc
      541 tagtgcaaaa tatagtgatt atgacaatgc ccgcgagaag cagactttat ggtggaagtc
      601 acaagcgggg aaacagcctg gagatccaaa gaaactggct cgtctgcttg ttaaaatcgc
      661 tcatctggat aatccaccaa aacgattaat tgctggaaat gatgtaatta agcttattga
      721 aagcaaaatt gaagaactgc agaatgatat ccagttttat aaaaaacttg atttttcact
      781 ggaatattaa ttctgtacat ttttataact aatcaataat gaaagagcag gtaattattt
      841 ggaagccaat atatttgaaa atcttaagtc gtacggaaaa gtattaaaag atgaaccaat
      901 gagcagttat acctccttta aaaccggggg caatgccgat tatcttgttt atccagcatc
      961 aattaacaaa tgtgccgaac taatagaata cttgaataaa tatgaatata acttcactgt
     1021 tttgggagga tgtaccaatc ttgttgtcgg agatatgggc attcgaggtg ttgttattat
     1081 catctctgat ttgacagaag aatttaatga aattagagtt attgacgata acactgttta
     1141 tgcgggggct ggtgttaaga aatcggactt tataaatttt accgcagaaa atgattttga
     1201 cggaatgcat tttatggttg gtattccagg gtgtatcggt ggtggcataa taatgaacgc
     1261 cggaacatat atggggacat tttcctcggt tttaaaaaaa gtctattata taaataatcg
     1321 tggtcagagc aaaagtaaag agatcgactc taccatgggg caatacagac atcttgactt
     1381 ggaagcagaa tgtgctgttg taacaggagc cgatttttcg ttgccaaaat cagataataa
     1441 agagttggtt atacaaaaaa tcaacgacat aatcgaagac cgcaagcaaa aacatccatg
     1501 ggattatccc agtgctggat cagttttcaa aaatcccgaa aacgaggcct cctggaaact
     1561 tgtaaatgat tgtggtctaa aagggtattc cataggtgga gcccaggttt ccgaaaaaca
     1621 taccaatttt attattaata agggtgaagc aaattctgcc gagattagag cacttgtttc
     1681 atatattcag tcacaagtaa aaataaaata cggtatagaa cttcataccg agataaggat
     1741 gataggcgag tttcattgaa cagttcttta tacgacaaaa ctttttgtgc tcttgatata
     1801 gagactaccg gagtaaatcc cttcaaggac aagataatcg agattggaat cgtacggttc
     1861 cgcataggtc aggagcagaa caatacatat caatctctta ttgacccatt aatccctgta
     1921 tccgaacaat cacgtcttat tcacaatatc tctcaagaag aacttgaagg taaaccccta
     1981 tattcggata tatattgctc tatcaaggag ttcattgaag attcaatact ggtgattcag
     2041 aacccccagt ttgatctatc ctttattcag tcagaaaaca gagccgctgg cttaccattt
     2101 ttcagtaaca gatctttcga tacggtatca ctcgctaaga aagtctttaa aggattaaaa
     2161 aatcataaat tagaaaccgt ttcaagacat ctaaattgta taaggaactt ccaccgagca
     2221 cttgacgacg cgttttgctg tatggatatt tttctggagg gtgtcaagag aactgatcca
     2281 ggtcgtattt ttactatttc agatttgcat aatatttgtg gctttgatat tcaggaacgg
     2341 gtctataagg atgttggtag ccgtaaaatt agaggagtaa acatatcacc agataaagaa
     2401 tacgagatca tatattgtga tcgggcaggg cagacaaccg aaagaaaaat actgtgtaaa
     2461 aaaattataa aaaatggctc cacttatttt gtccatgctt tttgctattt gagggagcaa
     2521 gatcgttttt ttaaattttc gcgtataaaa aatgcgacat tatgctgata ttcgttcgtt
     2581 tgttatacga ctagtgaaaa aattatttag ttgtagtcta agtaaaagtg attctaacca
     2641 ttcagaagat atgaatgaaa atggtttgct tttcttttct ttttcatttt taagaaaaat
     2701 cagaaaacca ataaatgctg agtgaatgta gtgggtagat gatagatcaa gaactatatt
     2761 ttcttcaact aactgatcac tcatcatata gagctcagga atctgctcaa aagtaatacg
     2821 atcagggaaa aaacatctat tattttttat tattatcatg tgattacccc catgttgtat
     2881 atcgtctgta aagcctcatt acttaagaaa gtttttttaa atctgataat atttggggtt
     2941 gttgttattt atctttgagg ttgccatgaa tcatctgaaa aatatacctt tatctattcc
     3001 ttataaagag atatatgcac gtcttgggta ttctgtacat aaaacatcgc tgcaaaaaag
     3061 cgagaaagac agaatagatg ctattatccg tgatggattt tcgctgtgta atttccaggg
     3121 aatttataaa caggttaagg ttttgtcagt aacccaaaag ggttttgtac tggaaagtgg
     3181 tcatgaaatt gagtctgtat accttaaaga caaacttaag gagtatggct caatgataat
     3241 gatggcagca tctgctggaa gaatagtgac cgaaacagtt acagaactta tgaagaaagg
     3301 agataacggc agagctgtta tttatgatgc aaccgctagt gaatcggcag atgcagttct
     3361 agattatctt caaaaacata taaagatgca gtttgccaga tctggaatgg caatttcacg
     3421 tttacgatat tctcctggtt atggagatat tgatctttca gttcaaaaaa taatttacga
     3481 tatacttgaa ttacaagact atggaataat tcttacagaa tattttcagc ttataccaga
     3541 aaaaagcgta ctagctattg cgggtttatc atgacaagag aagaatttgc acaactatgt
     3601 ctcaataaaa cggttattct tgatggctct accggaattg agttaatccg tcgaaaaatg
     3661 aaggtttcag gatcgataga gacatccatt ctcgattctc cagacatgct aaacgagctt
     3721 caactgcaat actgtaaggc cggtgcagat atcatactca cacctacgtt gggtgccaat
     3781 cgtgtgaaat tacaggagta ctccttcgat ggatcagtaa gagagttgaa tcgcgaactg
     3841 gcgtctctgt ctcttaaaac agcatccagc ttcccgacaa aagtctttgg cgatatatct
     3901 tcgaccggac gttttatcga accttttgga gaccttcttt ttgatgatgc agtggagtac
     3961 tttcgtgaac aagcagaagg tctgctcgaa gccggtgtaa acggtttttt tatagagact
     4021 atgatagacc ttcaggaggc aagggctgcg cttatagcag ttagagaatt atgtgacttg
     4081 cccgccgttg tatgtctgag tttttataaa gagggttata cccttaatgg cagttcaccc
     4141 cagagtgtag ttgtcaccct tcagagtctt ggtgccgatg cggttggatg taactgttct
     4201 accggacctg ctgaaatgat cccaattata caaaaaatgg caacttgtac aaaactacct
     4261 cttgttgtta aacctaatgc tggcttgccg agccttaaaa acggtaaaac agtatattcg
     4321 atggataaga atgagttttt aagttatatt cccaattttc ataaattagg agtttccctt
     4381 attggaggct gctgcggaac agatcctgga tttatcaatg atattgctgt ccaatggagc
     4441 aataaggtac ctgtaaaaag agaatctttt ataacgcacg ctatagcatc taaccgtact
     4501 ataaccagat ttggagccgg cagttttaca ctaattggag aacgaatcaa tccaaccggc
     4561 aagaaagatt ttcaaaaaca gcttagagaa aaagatttaa ctacaattga aatttttgcg
     4621 agcaaacaac aagagtttgg cgcgaatgtt cttgatgtga atatgggact ttcgggaatc
     4681 gacgaacgtg aaatgatgtt aaaaacaatt tcactactaa gtcgtatatc ttctttaccg
     4741 ttgtgtatcg atagcacccg acctgaggta atagaaagtg ccctgagact atatccggga
     4801 cgtgcacttg taaattcaat ttctggtgaa aaagatcgac ttgagattac gcttcctata
     4861 gccgcgaaat atggagccat gcttattatt ttaccactgg atgataatgg aataccggta
     4921 acttttgaag aaagggtact agttattaga aaaataatcg atgaagctac taaatacggt
     4981 tatacaaccg atgatatggt aatagatggt cttgttatga caatcagttc cgatcagaag
     5041 gccgcaaaaa ccacactgga tcttattaac tggtgtgcta aaaacgggat taacagtgta
     5101 tgcggtttat caaatgtttc attcggtttg ccacaacgag atatagtcaa cagtgctttt
     5161 ttggctttgg gcgcccaaag gggtttgact tcagcaatcg cgaatccttc cagcgaaaga
     5221 ttgcgtaaca ttatcagttc tattaacaag aagggatgcc gtatatgaat atattgacta
     5281 aacttttgga aaaaaacgaa atccttatac tggatggagc gactggaaca gagcttgctg
     5341 agcgaggaat gccttctggg gtatcgcctg agctatgggt tcttgataat ccatcatcaa
     5401 taaagtctgt acaaaacgac tattttaatg cgggtagtga tattgtttac agctgttcct
     5461 ttggtggaaa ccgaataaaa ctctctcaat atacagaaga tctttctgct gttttcgata
     5521 ttaaccgcaa attggctatt atatcaaaag aaaattctca cggcggtatt gtcttcggag
     5581 atatggctcc gaccggcaag tttatagaac cgtttggaga ccttgctttt tctgaagctg
     5641 ttgatgtgta ccgagaacag gcagcaggat tatgtgaggg cggtgtagat gctcttgtta
     5701 tcgaaacgat gatggacctt caagaaacaa gggcagcgct tcttggtgct aaacaggctg
     5761 ctggccaacg tccggttgtc gttaccatga cctttgacgc tgatggctat acattaaacg
     5821 gaactcatat cctctccgct ttgtctatta ttcagtcact tggagccgat gctttcggat
     5881 gtaactgttc tacgggtccg gccgaaatgc ttgaactaat aaagatcatt tctccctact
     5941 cgcgtatacc tttgatggcc aaaccaaatg ccggtttacc caagttgata gatggcaaaa
     6001 cggtgtttac catgggggct gaagagttcg cttcatattc aaaacagttt atcgatgcgg
     6061 gagtccgtat aatcggtgga tgttgtggaa ccactcctaa tcatataaaa aaagtaaaag
     6121 aagcattgga aaactatagc ccgtctgcca tatctgctaa aaaaagcttc gtaataggca
     6181 ctgttaagga tgcacaggtt gcctcggtcg acgaaccttt gatgttgatt aatactgaag
     6241 ccggtgataa atatcgcaac tgcagcgcag atgaacttag tgcttctctt gtcgaattct
     6301 gtatacataa tagctacata ccagtgataa aatatgataa cgaagcaaat ctttcatttg
     6361 ctctgaatgt ctatcctgga cgagccttga ttgttgtaga aaacgagcaa gactttcaga
     6421 ctgttttgcc cctggcagaa aaattcggtg cagtaattat tacggacaaa tttgctccac
     6481 aaaatgtttc aaatacacca tgcttaaccg atattgttgt aatctgtaat gtaacaaaaa
     6541 atgagaatta tctaaatgct tataagttat ctagcatggg tgaagaagat gaaaggttct
     6601 ttgcagtatc ggagctttac aataatgaga ttcctgtatt ggttggtgat ccgacagatg
     6661 accttcgcga ctttatagtc gcgtatgacg cgttaacagg tcgggacaga aaacttgaag
     6721 gattgatgaa caggcttcac caaactgata ataagcagat atcggatcaa gaaatcgaag
     6781 aaagagatcc tgtttatatg gctgttttaa gaggacgtga taatgtaata gaaaatgcca
     6841 ttggcgattc acttaaagcg ggtatctcac cgggtaagat cgtagatgaa tatctgatac
     6901 cagcaattaa tgtagttggt gatctttacg aaaagaaaga atattttttg ccacagctta
     6961 ttatgagcgc agataccatg cgaaaaggat ttaaaattct tgaaccactt cttgctgcag
     7021 aaaacagctc aaaaagcaag gatggtctta ttgttattgc caccgttaag ggtgacattc
     7081 acgatattgg taaaaacatc gttgccctga tgcttagaaa ttataatttc gatgtgatag
     7141 acttgggaaa agatattgat gataaaacaa tagtagattc ggcaattaaa aacaaagccg
     7201 atattattgc gctctcagct cttatgacaa caaccatggt gcagatgaaa actgttgttg
     7261 atacagcaaa aaaatcaggt tgtaaagcca agataataat tggcggtgct gtggttgacc
     7321 agaattactg taccgagatt ggagcagatg gttactcttc tgatgcgatg gaagcggtaa
     7381 aacttgctca aaggctggta tcagcctagt ttcattttat ttcttgattc ttgttgaggc
     7441 ctcatataaa agaattgatg ctgcaacaga agcgtttaaa gagcttacct taccggttag
     7501 ctctattcga acgaccaggt cacatttttt ttctgttaaa tcacgcatac cagttccttc
     7561 acttccgatt acaatagcga gtggtgagta ttcagatatt tttgacatgc tttcctgacc
     7621 tttatctgat gacccaataa tccaaaaacc cttatctttt agtaaatcaa ttgttcgtgc
     7681 aaggttagtt actttcatta atggcatata ggcgcttgct ccggcagaac tctttataac
     7741 cgtggatgta actgaagcgc tattatcttt ggtgattatt acccctttac agccaaatgc
     7801 ctcggccgat ctgattatcg caccaatatt atgaggatcg gtaagttgat ccagtatcaa
     7861 aacgattcct ttgtttgaag caatagcctc aagttgattc tcttcaaatt cggggtacag
     7921 aggacagttt attagtgcaa ttccctgatg atttttatca ccaccgcaca acttctccaa
     7981 gcgatccttg tctgctcttt cagatctaac acctcttttt ttagagagtg atgttatttc
     8041 ggtaagtgaa ttctctttta taccagatgc aatcaaaaga gtagtagatg atgtggccag
     8101 acctgaacgc agatactcaa gtacaggaat tcttccaaaa actataccgt tttccatcgt
     8161 gtaccttcct tggaatcttc cagaacaact cccttatcca aaagaagatc tcttatccgg
     8221 tcggcttcat tccagttttt atcttttttt gcattatctc tttttattat ataatcttct
     8281 atttcttgtt cagaaagccc ggctttcttc tcttctttaa aaaaaataat acctaaaaca
     8341 gtgtcaattt tctcaagaca atcaagaatt aacctggcct cttcagatga gaaattgctt
     8401 tgtgtgtaat acttgttaaa atcggttact gcttcaaaaa aatgccccaa agctccagat
     8461 acatttagat cattatctag agatgacgta aaatcattta taagcttatc tattcgatcc
     8521 ttaatgagtt cacgaacttc accgctcttt tgagattttt cacaattaag aacaaggtta
     8581 tcaattcttg aaagtggctg agaggcattc ttaagattat caagcgtaaa gttaaactgt
     8641 ttacgatagt gagcagttag aagaaggtaa cgtatggcgc gtgatgaaaa gccttgttcg
     8701 ataagatcat ttaatgtata gaaatttcct ttggatttag acatcttgct gttatctaca
     8761 agtagatgct ccgcatgaag ccagtatctt acaaatggtt catcgaaagc tgcttcactc
     8821 tgtgcaatct cgttctcatg atgaggaaac acaagatcaa caccaccggt atgaatatct
     8881 attgtggtat tatatatttt tcgtatcata gccgaacact ctatatgcca acctggacgt
     8941 ccataaccaa ggtcaatctg ccatcccggt tcatcttttg agacctcttt ccagagagcg
     9001 aagtccctga tgtcatcctt atggtactcg tcagtatcgt accttgtacc cgacttgatt
     9061 gattcgggat caattttact taatttgcca taatcagaat atttggaaat actaaaatag
     9121 attgagttat tatctttata aaccagacct tttttttcaa gtcggttaat gatgtcattc
     9181 atcgcatcga tagaatccgt agcgcgtggg tagtgttcaa attcttcaat atttaatttt
     9241 tttaaatctt caaaaaatat atctgtaaat cttgcagtat attctttaag ggaaactccc
     9301 tctgaaagag cgcctttaat ggttttatcg tcaacatcgg taatgttcat agtatggtta
     9361 actttaaaac ctctaaattt caagaagcgt cttaaaatat cattaaaaag aaaagttcta
     9421 aagttcccta tgtgagcaaa tccataaaca gttgggccac aagaataaat tgaaactgga
     9481 gggtaatcat taattgactc ttttttctct cctcccggaa taaattcctc tattttttta
     9541 ctcattgtat tatagaattt taccatttcg aatcctccgg aaaaacttaa aaaagagatc
     9601 ttataacact aatctataaa atgatataga attacttttg gctaaaaacg tttgtttgaa
     9661 ttttatatta gttatgactc ataacaaaaa tgttgcggta catgattatg tcaaacagtt
     9721 ttgtcttaat tagctaatgg ggttaatatt aagataatat ctcgtttccg tcattatcca
     9781 atattatttc aaccgtgtcg gtatactgat cgacgattgt ttgagaagaa gaggtctgat
     9841 ttgtgccggc aggacgagaa gccagattta tttcaccgta tttatatcgt ttttttacta
     9901 ttctaaaatc gatattatgc tctcgtaata gattttccag tagttttata tagcctaaca
     9961 gaattttaat tttttcattt ttccttttca aagctttatt catctgtttc gttattgaaa
    10021 gtagaatgtt ttctctgttt tttaattttt ttttcaattt tttgataatc tgatcagttt
    10081 tgccatcgac cgagactagc tcgacgtgag cataaatctt gttatcattc ccgttttggc
    10141 gggaagcttg tatgaaattc tttatccgtt tatctgacaa aatatgagaa ggaagtgtct
    10201 tttcccactc agtttcgttc acggttaatg tctctatggc tttggttgta tccattatat
    10261 tacaatcact ttctttattt gagctcgttt caatgaataa ataatgctaa aatagtaaaa
    10321 taattcatct aaataacacg gataattgtg ttttattaac agtatcaact atttataaaa
    10381 atagatatac tgttcttatt gtcggatttt attataacat aatttaccta aaagatttat
    10441 cgcatcttgt attttaccat taaatattaa aaaaggaaaa atagcagatt ccgaatacag
    10501 aagttagatt atattagttc ttgacttaac aacatttaaa aataaatact aaattaagct
    10561 ttaaaagtcg gccctacata ttcttaatag tctaaattat ttcatacttt gataggcaag
    10621 ttattatgta tacagttttg cacattgaca acagccattt ttttaataaa attgtgcgtt
    10681 catgtatcga gaaaattgga ttaacttgta taacctctaa cgacatctct gaagctgttg
    10741 aaaagcttga aaataatcat atagatctta ttataacggc tcttgaaatc cgtggtggtg
    10801 gaggtgagta cctgataaga cagttaaaca gcggaaaatt tcgcaatatc cctgtaatcg
    10861 ttatgacctc aaacgactcc atgagtactc gtattaagat gtttaaactg ggagtagtcg
    10921 attatatatc caaagaccag accttcgcgg gagctctatc aggattttta aataaactca
    10981 ccgaaaaaaa tcgttccaga tctcttctta aaaagttatc ctttgctgtt cttgatgatt
    11041 ctaaaacgca acttcaggta ataagcaata ttatgtcgct taacaagata gaaaatgtct
    11101 cctttttttc taaaggaggt gacttactta aatccaataa caggtttcat atatattttg
    11161 tcgatcttgt attgccagat atttcagggg aacaggctat tcttcaaatt cgtaagaaat
    11221 acccggaatc cttgattatt gccgtttcag gaattgataa ctataaagtt gtatccaacg
    11281 tgcttctttc gggagcggat gattatattc taaaaccctt taactcaagt atatttatgg
    11341 caaggctcgt ctcgaatgtc agggtatttc aacttgtaca ggatttaaaa aagaaaaatc
    11401 aggagcttga agagagccta atcactgatc ccctaacagg actatacaat catcgattta
    11461 tttacagcag acttcaggat attatcagta aaaaaacatc acctttctct tttgttttct
    11521 tcagtgtcga taattttaat aatattaacg atacctatgg tcatagtcaa ggtgacaagg
    11581 ttctttgtaa aatttccgat gtactgttgg gtgaaatcga agatagatca tgtataggaa
    11641 gatatggagg agtagaattt attgttcttt tcccgggcat gactgtagat aaagcatttc
    11701 cagttgcaga aaaaatccgc caaagggtaa atagaatcac agtaaacaac gcaagaatat
    11761 ctgttagtgg tggcctttgc caaaacgaag gtgaagagag ggcggaagat cttatacgaa
    11821 aggcagattc gcttttgttt aacgcaaagc ataacggtaa aaaccgaata gaaaaaagtt
    11881 ttgactggca atcctattta taaagagagt ttagtccttc cataccatca ataacagtat
    11941 cgtaactgtt ttttgcgttc cagaaaatgt atccttctga tttggtatca atgctggcct
    12001 tgatttgctc ctgtatataa gaagcatcat aattcgatac tctccaacca aatgcctgaa
    12061 gccagggacg aaccattgtt ttaccagact gttttttaac tctggttaca ccttccgaaa
    12121 taaagtagta aggagaatca cctggtttcg caatacctct gaaattgttg ttaaaatgag
    12181 acggataaag cattgggcat ataatatcac aatattgcga aagcttggc
//
LOCUS       DAOTGZ010000067        11992 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_3064
            NODE_6344_length_11992_cov_4.982324, whole genome shotgun sequence.
ACCESSION   DAOTGZ010000067 DAOTGZ010000000
VERSION     DAOTGZ010000067.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868088
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 11992)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 11992)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:22:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,785
            CDSs (total)                      :: 2,751
            Genes (coding)                    :: 2,732
            CDSs (with protein)               :: 2,732
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 12 of 19
            Pseudo Genes (internal stop)      :: 5 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11992
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6344_length_11992_cov_4.982324"
                     /isolate="AMR_MDS_3064"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            234..905
                     /locus_tag="P5123_06090"
     CDS             234..905
                     /locus_tag="P5123_06090"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRX15863.1"
                     /translation="MFDIKEYYLENSLLTESVKKGASPETSYDDAVSQDIKPILINPQ
                     KSGYNNELFKRGKHSINIYTPSVYCPFAGEFSVEPDYLRFLLSIYPKKDELLPIKHII
                     ARPRFIESGRIQLVSLFIPSPGCIVLYLSYPHFYTFNNGEFFGKESFSYDLSSIQNPH
                     YLGTTAPESQILSVPPLFYIIKSITKKLDYTIDKYLLRLNNSANEQTLVRLDEISHFY
                     NRHGY"
     gene            908..1642
                     /gene="pgeF"
                     /locus_tag="P5123_06095"
     CDS             908..1642
                     /gene="pgeF"
                     /locus_tag="P5123_06095"
                     /inference="COORDINATES: protein motif:HMM:TIGR00726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan editing factor PgeF"
                     /protein_id="HRX15864.1"
                     /translation="MMHLDFEKKDSNCLLVKQQNDKFLIGVAAKNCNTVNYELDFAKI
                     RKYEKYCIHFETGTTPENIYFINQQHEDNCVDIRTAADISLPYFADADAMITGQKGAC
                     LVIRTADCVPVLIADTSGKCIGAIHSGWRGTEKNVTAKTISCICDSYGSKIEDLRAYI
                     LPSIGVESYQVDEEVASIFPDSSKKMNDSWHLDLRHAVVKQLLDMGIHSNNIFASPYD
                     TFSSNELFFSHRKGDKGRNLNFITLK"
     gene            1927..3270
                     /locus_tag="P5123_06100"
     CDS             1927..3270
                     /locus_tag="P5123_06100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012474563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ammonium transporter"
                     /protein_id="HRX15865.1"
                     /translation="MRNKLTGLLLILFSASGLFAESPELSETVKESFFALDNLFIFFC
                     AVLVIFMQPGFALVETGFNSSKNAVNILFKNVIDLCVGVLLYFFVGYGLMYGAPIIEG
                     FFAWGGSGIADFDGVVEGGALLPQVDFLFQVAFAATAATIVSGAVAGRLNFKAYLIYS
                     AVITGLIYPISGFWKWGGGWLDAMGFYDFAGSIVVHSVGGFAGLAAAIVLGPRIGRFA
                     KDGTPKAIPGHNLPLATLGVFILWVGWFGFNPGSQLAIAGKANTEAVMLIATNTTLAA
                     AAGGLFAMILTWILHKKPELSMALNGILAGLVGITANCDGVTNIEAIIIGSVAGILVV
                     LGIKLLDRLRIDDPVGAWPVHGLNGLWGGIATAIFAEYGPIEEGSSIIYGNTIAQLTG
                     SIVIPIWAFATMFVLFVALKKIGILRVSREEELKGLDIGEHGEEAYNGFQIFTIE"
     gene            3285..3626
                     /locus_tag="P5123_06105"
     CDS             3285..3626
                     /locus_tag="P5123_06105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011890028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="P-II family nitrogen regulator"
                     /protein_id="HRX15866.1"
                     /translation="MKLVTAYIRPEKLNDVKEALGDADIKKMSVTNSLGCGQQGGYTE
                     TYRGVDMQINLLKKVRIEIALNEDYVETAINAIIKGAKTGNIGDGKIFVTDIIRCIRI
                     RTEEEGEKAIG"
     gene            4046..4579
                     /locus_tag="P5123_06110"
     CDS             4046..4579
                     /locus_tag="P5123_06110"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HRX15867.1"
                     /translation="MKKKCLNAAIEMLYKNHHQFVVMMLYRKILDIHLSEEFAHDIFV
                     KIIEGGYIVNIPSSLQRKYLSKCIKNLLSDYYKALNSHNHESYTDNMLLTYNRNKTTE
                     NCFLEGEIMSTLHDALDKKMTDQEMYIIRKKYFENENITTICKKIKLSRYRFRRILRK
                     ATDKVREELTPYFQISR"
     gene            4645..5643
                     /locus_tag="P5123_06115"
     CDS             4645..5643
                     /locus_tag="P5123_06115"
                     /inference="COORDINATES: protein motif:HMM:NF014610.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 4-thiouridine(8) synthase ThiI"
                     /protein_id="HRX15868.1"
                     /translation="MQKKGILLFSGGLDSLIAAQVLIEQGITVKAYNFLLPYEKPDYN
                     PYQSAIAKSAHGLDMELNFVQCGDDYIKMLLDPPHGYGKNINPCIDCKILFLRKAKEI
                     MINEDAAFIATGEVNGQRPMSQRKDMIIHIEKSAGLERMILRPLSARFFKPTLAEEAG
                     IVNRDALLDINGRGRKIQMEMANKYGFTDYATPAGGCLLTEPHYSVRAKELFATNKSI
                     TSRDLYLLRLGRHFRIEGVKIVVARNADETNNLINLRNNNDTLFSTQFQGPSMLSPGN
                     SKLSDSVKKTAAAIAARYGKPGKEDHEREVIVYERDGQETIIAGSIADEQINCFKI"
     gene            5765..6352
                     /locus_tag="P5123_06120"
     CDS             5765..6352
                     /locus_tag="P5123_06120"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRX15869.1"
                     /translation="MRLILFIISFCIITPLSILVHHYGTLFMMKTEYTRDDFTNLDPV
                     KTRILNRLGNAPNRTVTIIVSFVALVLPYFLTATMLNWFINSVILYALFYYARFLLLP
                     KNEDTGLPFDIDLVMIVFGFSLLAGFTLNWISFMNTGFLFFAVNFFATIILLYYSLTL
                     FFSQQSKPVSKSGDDSATVQVNPKTDSDQNGENNE"
     gene            6345..6836
                     /locus_tag="P5123_06125"
     CDS             6345..6836
                     /locus_tag="P5123_06125"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRX15870.1"
                     /translation="MNKLLLLPIIFLMMISLISCGSKPIVRPEPAPEPIKPVKEPAEI
                     KMLAEGWIGEDIYRAKSVGTPSEGITGAEEIRKSALEDAVNIAQRRIVEKFVTTRITG
                     LSETDDIMSTGVAIQKEFGPAIRSGKIVQEIYKSDGSCIIMYEVELKNLKRRVSDAHF
                     KEK"
     gene            7030..8958
                     /locus_tag="P5123_06130"
     CDS             7030..8958
                     /locus_tag="P5123_06130"
                     /EC_number="4.2.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008441971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aconitate hydratase"
                     /protein_id="HRX15871.1"
                     /translation="MGQTLTYKILQKHLIEGELKAGSEIGIRIDQTLTQDATGTMAYL
                     QFEAMGIPRVKTDLSVSYVDHNTLQMGFENADDHKYLMTMNNKYGIHYSKAGNGICHQ
                     VHVERFGKPGATLLGSDSHTPTGGGIGMIAIGAGGLDVAVAMGGGPFYLTCPKVINVK
                     LEGKLRPWVSAKDVILRVLEILTTKGNVGCVVEYSGNGVSQLSVPERATITNMGAELG
                     VTTSLFPSDDITKEFLKAQQREDHWSELKADEDAVYDRVIDIDLGDLKPRVACPHSPD
                     NIKLVSELDGLKVDQVAIGSCTNASYKDLMIAASILKGKKVSPDVSLIVAPGSKQVFE
                     MISRNGALSDLIASGARIMESACGFCIGAGQAPVTEGVSVRTNNRNFEGRSGTKSAGI
                     YLVSPETAAVTAIKGVLTDPSTMPENEYPKVTIPESFIIDDSMVLKPDFKGDVFRGPN
                     IGEPPFTEELAEKIIGKINLKVGDKITTDHIMPAGARLKYRSNVPAYSRYVFEGVDPE
                     FSSRAMADKEKGIYSIIVAGASYGQGSSREHAAICPMYLGVRAVVAKSFERIHSANLI
                     NFGIVPFIFKNEADYDTVKQSDDFIIDGIRIAVESGSETATALCGTKKIELSMTLSKR
                     QRDILLAGGLLNHTKAQA"
     gene            9279..11228
                     /locus_tag="P5123_06135"
     CDS             9279..11228
                     /locus_tag="P5123_06135"
                     /inference="COORDINATES: protein motif:HMM:NF014325.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-binding protein"
                     /protein_id="HRX15872.1"
                     /translation="MKPKQSERLAYWFDNYMAKGPRSIFFALLVVFAAGFITLAIIRV
                     IVGFYVIEGTTGDVQTELGVWRIFLQLTDPGNMAQDNDSYWFIRIFAVISGMFGIIFF
                     SAVIAFITTQLDLKLEALKKGRSRVLENGHVLLLGWSNEIFDIIRELIIANESEASAA
                     IVVLSDVEKEEMDDYLNEQITDRKTTKIITRTGEAGSIKALERVAVTKAKSAIILPTC
                     THGATDAEKAHSDAHTLKSTLAVVAACQSKEELPSIVTQVYNESNREIIRSLAPDDIT
                     IVDPEDMIAKIIVQTSRSSGLAAVYSSLIGFDGCEFYFTNAPWGELPFKDVHFHYPDG
                     VAIGVCHKDGSIKINPENDYILKKDDDIIILAEDDSTIHYKNRSICTPTKIDLSDKRI
                     PSAKEKVLIIGWNNKAPVIISEFMDYITDDSEIIIVIPPDVTTESEALLQIKADNPNF
                     AIKTVKVDLLDLQQLEELSPESFNSIIILTANEDNDIERADAATINILLMLRTVIRRK
                     AAKGKAPQIITEVLNSENLELISQAGVNDAVISTKMVSKILSQIAEEPDILQVYEEMF
                     TEDGSEIYLKPLELYFKTMPSTVTYADLIALSHLREEICIGYRLKENIMSAKDDFGVI
                     LNPSKTETIEVEKIDSLIVIAENEL"
     gene            complement(11266..>11992)
                     /locus_tag="P5123_06140"
     CDS             complement(11266..>11992)
                     /locus_tag="P5123_06140"
                     /inference="COORDINATES: protein motif:HMM:NF024206.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ankyrin repeat domain-containing protein"
                     /protein_id="HRX15873.1"
                     /translation="LAAMGGVSSDPVEARFTSVVEELLKEGVPVNLSDSSGKSLLVYA
                     IQSDSPMMVQTVLKHNPDLSYRQSPFGPTALHYALSGANPIIIRMLVSAGADINAITR
                     NAEYRPAPFETSSTQANRYKKPGEYNLKIEVYSDKKNPAQDRRCFYKVFIKKVESGRT
                     VTGLESQKKSFTTSIDINRHHISVVKYVLDERQGKYIKLNNIHQPRPSAFYTDTVEGR
                     IRVVSIIHNEETETSYYEDYEKE"
BASE COUNT         3808 a         2423 c         2355 g         3406 t
ORIGIN      
        1 ccaaaaaaaa ctattgaaga gtatataatg gcacatatcc tctcttcacc aaaatgggca
       61 ggaacaaact gatatggcct ctatttatta acggtatcaa ccaccgacat ttttttctct
      121 gattcatcag taaaatttat aaccaatata aaaaaaccgc aacacattga tttatttctg
      181 aaatttggta atggctctta gttgccagtc aataatcatc aagaggtaca taattgttcg
      241 atattaagga atattatctc gaaaattcac tgcttacgga atcggttaaa aaaggagctt
      301 cccctgaaac cagctatgat gatgccgttt cacaggacat taagcctatt ttgattaacc
      361 cgcaaaaaag cggatacaat aatgaacttt ttaaacgggg aaaacactct ataaacatct
      421 atactccttc agtctactgc ccctttgccg gtgaattttc tgttgaaccc gactatctaa
      481 gatttttgct ctctatttat cccaaaaagg acgaacttct gccgataaag catataattg
      541 caagaccccg cttcattgag agcggacgga ttcagcttgt ttcacttttt attccatcac
      601 ctggctgcat tgtactctat ttatcatacc ctcattttta caccttcaat aatggtgaat
      661 ttttcggtaa agagtctttt agctatgacc tctcttcaat ccaaaacccg cactatttag
      721 gaacgactgc tccagaaagc caaatattga gcgtgccacc acttttttac attataaaat
      781 caataacaaa aaaactggat tataccattg acaaatacct gctgcgttta aacaattccg
      841 caaacgaaca gacgcttgtt cgtcttgatg aaatatcaca tttttataat cgacacgggt
      901 actaaacatg atgcatcttg attttgagaa aaaagattca aactgtctgt tggtaaagca
      961 acaaaacgac aaatttctta tcggagtcgc tgcaaaaaat tgcaatacgg tcaactacga
     1021 gctagatttc gcgaaaatac gcaaatatga aaaatattgc attcactttg aaaccggaac
     1081 aaccccggaa aacatctatt ttattaatca gcaacacgag gacaattgtg tagacattcg
     1141 caccgcagca gatattagcc taccctattt tgctgacgct gatgcgatga ttaccggaca
     1201 aaaaggagcc tgccttgtta taagaaccgc agattgtgtg ccagttctta tagcggatac
     1261 aagcgggaaa tgcataggag ccatacactc cggctggcgc ggcacggaaa aaaatgtaac
     1321 tgcaaagacc atatcttgca tttgcgatag ctacggttca aaaattgaag atttacgcgc
     1381 ctatatttta ccatcaattg gcgttgaatc ttaccaggta gacgaagagg ttgcatccat
     1441 tttccctgac agctctaaaa aaatgaacga cagttggcat ctggatctca ggcatgctgt
     1501 cgtaaaacaa cttttggata tgggtattca cagtaacaat atattcgcct caccttacga
     1561 tacattctca agcaatgagc tcttcttttc tcaccgcaaa ggagacaaag gcagaaatct
     1621 taattttata acgcttaaat aaatcaaaac gatacaaaaa tgtaattttt tacaaccagt
     1681 gttacatttt tgtatcatac ttcctctttt taaaacaacc aaattgaata ttactataaa
     1741 cttcttactg gttaatccct ctttatgctt taaataaccc tttggcacag ttcgaagcag
     1801 ctaaatattt caattttgtt aaataacgtt tataatcccg caaaatcagt ttaaaaaatg
     1861 acacgtcaat ttttcggcat actacatgca actgatattg cataacattt atgcaggagg
     1921 aaaacgatgc gtaacaaact aactggtctg ctcctgattc tcttcagtgc gtcaggacta
     1981 ttcgcggaga gcccggaact ctccgaaact gtcaaagaga gtttttttgc tctggacaat
     2041 ctttttattt ttttctgtgc cgttctggtt attttcatgc agccgggttt tgcactggtt
     2101 gaaacaggat tcaactcatc aaaaaacgct gtaaacattc tgtttaagaa tgtaattgat
     2161 ctttgtgttg gggttctact ctatttcttc gttggctacg gtcttatgta tggagcacca
     2221 attattgaag gtttttttgc atggggcgga agtggtatag ccgattttga tggtgtagtt
     2281 gaaggaggag cacttcttcc acaggtagac tttctgtttc aggtagcatt cgcggcaaca
     2341 gcagctacaa tcgtttcagg cgcagttgcg ggaagactaa actttaaagc ctatcttata
     2401 tacagcgccg ttattaccgg tctgatttac ccaatttcag gattctggaa atggggtggt
     2461 ggatggcttg atgcaatggg attttatgat tttgctggat caattgttgt tcactcggtt
     2521 ggcggttttg caggtcttgc ggcagcaata gtgcttggtc cccgcattgg gcgttttgca
     2581 aaagacggta caccaaaggc aatacctgga cacaaccttc cacttgccac actgggtgtt
     2641 tttattcttt gggtaggctg gtttggtttt aacccaggtt cacagctagc aatagccggc
     2701 aaagccaata cagaagccgt aatgcttatt gcaacaaata cgactctagc cgcagctgca
     2761 ggtgggcttt tcgcaatgat cttgacttgg atacttcaca agaaacctga attatccatg
     2821 gcacttaacg gtattcttgc tggtcttgtt ggtatcaccg ctaactgtga cggtgtaacc
     2881 aacattgaag caataattat cggatcagta gcaggtattc tagttgtact tggtattaaa
     2941 ctattagacc gtttgcgtat cgatgatccg gtcggcgcat ggcctgttca cggacttaat
     3001 ggactttggg gtggtattgc aactgccata tttgccgaat acggtccaat tgaagaaggt
     3061 tcttccatta tctatggcaa taccattgcg cagctgacag gatcaatagt aataccgatc
     3121 tgggcgtttg caaccatgtt tgtgctcttt gttgctctta agaaaatagg aattttacgt
     3181 gtttcaagag aagaagagct taaaggatta gatatcggag aacacggtga agaggcctat
     3241 aacggattcc agatatttac catagaataa aaaggaggaa attgatgaaa cttgtaaccg
     3301 catatatccg accagagaaa cttaatgatg taaaagaagc tctgggtgac gctgatataa
     3361 aaaagatgtc cgtaaccaac tctcttggct gcggtcaaca gggtggatat accgagacct
     3421 accgcggtgt tgatatgcaa atcaacctgc tcaaaaaggt tcgaatcgaa atagctctga
     3481 acgaagatta tgtcgaaact gctattaacg cgataattaa aggtgccaaa accggaaaca
     3541 tcggtgacgg taaaattttt gtaaccgaca ttattcgatg catcagaatc cgcactgaag
     3601 aagaggggga aaaggctata gggtaatacc aacaaagccc taccttctca tggggccgcg
     3661 taagcggccc ctatttcgtt aaaaaaagcc cctttgtgct attatttcat atctctaaaa
     3721 ttcaaataag cttatttttg aattttttta taattaaaaa tacatttttt tattattaat
     3781 aaattttttt ttacaatttt taatatttat ccgtttatat atatatggtt attttaaaag
     3841 ccattgtaat cctgaccgcc cacagtgcag tgcctatcag atatcaggca ctgcactgtc
     3901 ttttcataca accgatccaa acaactgtta tttattttac tatttaattt tattattcga
     3961 ttgcaatcat gattatttat taatccacca atatataaat aatcaaagag taacagttta
     4021 acgttaacac acaaaaggga tagttatgaa aaaaaaatgt ctgaatgctg ctatcgaaat
     4081 gctttataaa aatcaccatc aattcgtagt aatgatgtta tacagaaaaa ttctggatat
     4141 tcatctatct gaagaatttg ctcacgacat ttttgtcaaa ataatcgaag gaggatacat
     4201 agttaacata ccatcctccc ttcagcgaaa atacttatca aaatgcataa agaatctgct
     4261 ttccgactat tacaaagctt taaactccca taaccatgaa tcatatacag ataatatgct
     4321 tcttacatat aatcgtaata aaactacaga aaactgcttt ctggaaggcg aaatcatgtc
     4381 aaccctgcat gatgctcttg acaaaaaaat gaccgatcag gaaatgtaca taataagaaa
     4441 aaaatatttt gaaaatgaaa atatcacgac tatatgcaaa aagatcaaac tgagccgata
     4501 cagattcaga cgcatcctca gaaaggccac ggacaaggtt cgtgaagagc ttacaccata
     4561 ttttcaaata tcaaggtagc ctcatacatt tcctgggatt attcagaata ttattttcaa
     4621 gaccttttat gggagtcata caagatgcaa aaaaaaggga ttcttctttt ttcaggaggc
     4681 cttgacagcc ttattgcagc acaagttctc attgaacagg gaattacagt caaagcctat
     4741 aattttctgc ttccctatga aaaaccggat tacaacccct atcagtcggc aattgcaaaa
     4801 tcggctcacg gactagatat ggaacttaat tttgtgcaat gcggtgacga ctacataaaa
     4861 atgcttcttg atccacctca cggttacgga aaaaacatta atccctgtat tgactgcaag
     4921 atattatttc ttagaaaagc aaaagagata atgataaatg aagatgcagc ctttatcgct
     4981 acgggtgaag taaacggaca gcggcccatg tctcaacgca aagatatgat aatacatata
     5041 gaaaaatccg ccggtctgga aagaatgata ctaaggccat tatcggccag atttttcaag
     5101 ccaactctgg cagaagaagc cggaattgta aaccgtgatg cgcttcttga tataaatgga
     5161 agaggccgta aaatacagat ggaaatggca aacaagtatg gtttcacaga ctatgcgaca
     5221 cctgccgggg gctgtctttt gaccgaacca cactattcag taagagcaaa agagcttttt
     5281 gccacaaata aatcaataac aagtcgtgat ctgtatcttt tacgtttagg tcgacacttt
     5341 agaatcgaag gggtaaaaat agtcgtagcc cgaaacgctg atgagaccaa taacctgatc
     5401 aatcttagga acaacaatga cacactgttt tcaactcagt ttcaggggcc ttcaatgctc
     5461 tcaccaggaa acagcaaact atctgattca gtaaagaaaa cagcggcagc aatagctgct
     5521 cgttatggga aacctggaaa agaggatcac gaaagagagg taattgttta cgagagagat
     5581 ggtcaagaaa caattattgc aggttcgatt gctgatgaac aaataaactg cttcaagata
     5641 taaaaagtat ttgattaaaa acattatcca cgttaaatta aataaggaat cattcggtaa
     5701 tgggatatga gcctctcttt attttacatt accatattaa ttccgctatt accagggagg
     5761 atgtatgaga cttatacttt ttataatatc tttttgtatt attactccgc tttcaattct
     5821 tgttcaccat tacggaaccc tttttatgat gaaaaccgag tacactcgcg atgattttac
     5881 caatctggat cctgtaaaaa cgagaattct aaacagactg ggcaacgctc caaaccgtac
     5941 agtcacaatt attgtatctt ttgtggcact ggttttaccc tattttttaa ctgctacaat
     6001 gcttaactgg ttcatcaatt ccgtgattct gtatgccttg ttttattatg cgcgattcct
     6061 tcttttacca aaaaatgaag atactggttt gccatttgat atagatctgg taatgattgt
     6121 cttcggcttc tcgctacttg caggattcac actcaactgg atatcattta tgaatacagg
     6181 ttttctgttc tttgctgtta acttttttgc gactataata cttctgtact attcattaac
     6241 acttttcttt agccagcaat cgaaacctgt ttcgaaatct ggcgatgatt ctgcaacggt
     6301 acaagttaac ccaaaaaccg attcagatca aaacggtgaa aacaatgaat aagttactac
     6361 ttttaccgat tatttttcta atgatgatat ccctgatatc gtgcggttca aaacccatag
     6421 tacgaccgga acctgcaccc gaacccatta aacctgtcaa agaacccgcc gaaatcaaaa
     6481 tgctcgcgga aggatggata ggcgaagata tctacagagc caaatcggta ggcactcctt
     6541 cggaaggtat aactggagct gaagagatac ggaagagcgc tttggaagat gcggtaaaca
     6601 ttgcacagag acgaattgtt gaaaaatttg ttacaacaag aataacaggc ctctctgaaa
     6661 cagatgacat catgtcaact ggtgttgcca ttcaaaagga gttcgggccc gccattcgct
     6721 cagggaaaat agtacaagag atttataaga gtgatggctc atgtataata atgtacgaag
     6781 tggaacttaa aaaccttaaa cgccgtgttt cagacgcaca ttttaaagaa aaatagtctt
     6841 atttcttttg tttgcaaaac agctgttgct taccactttt tacagctgtt tttttaaaaa
     6901 gtcttgataa cagacagcca tggtaaacta tggtctatcc ttcctgctcc gctctcaact
     6961 cctttctcct tttttttacc tccatcgatt gcggggcggg atggacttaa taaccttaag
     7021 gagtatcata tgggacaaac attaacatac aaaattttgc aaaaacatct tatcgaaggc
     7081 gaactaaaag ccggatcaga gattggaatc cgtattgacc agaccctgac acaggatgca
     7141 actggaacca tggcctacct tcaatttgag gcaatgggaa ttccccgtgt aaagactgat
     7201 ttatcagtct cttatgttga ccacaataca cttcaaatgg gtttcgaaaa cgcggacgat
     7261 cacaaatatc tgatgaccat gaataataaa tatggtatac actactcaaa ggccggaaac
     7321 gggatttgcc accaagtaca tgtcgaaaga tttggaaaac ctggtgcaac actgcttggt
     7381 tctgactcgc acacccctac cggtggcgga atcggcatga tagcaatcgg cgccggcggt
     7441 cttgatgttg cagttgcgat gggcggcggt cctttttacc tgacatgccc taaagtaata
     7501 aacgttaaac ttgagggtaa acttcgccca tgggtatccg ccaaggatgt tatactgaga
     7561 gtacttgaaa tactaacaac aaaaggcaat gtcggctgtg ttgttgaata tagcggaaac
     7621 ggcgtatccc agctgtcggt tcctgaaagg gcgacaataa ccaatatggg agcagaactg
     7681 ggagttacaa cttccctctt tccctctgac gatataacaa aagaattttt gaaagctcaa
     7741 cagagagaag atcactggag cgaattaaaa gccgatgagg atgcggttta cgaccgggtt
     7801 atcgatattg atctagggga tctaaaacct cgtgttgcct gtcctcactc tcctgacaat
     7861 atcaaacttg tttccgaact ggacggcctt aaggttgatc aggttgcaat aggaagttgt
     7921 acaaacgcct cttacaaaga cctgatgatt gccgcgtcta ttttgaaagg caaaaaggta
     7981 tccccagatg taagccttat tgtggctccg ggatccaaac aggtttttga aatgatctca
     8041 agaaacggtg cgttgtctga cctgattgct tcaggcgcaa gaatcatgga gagtgcctgt
     8101 ggcttttgta ttggtgctgg tcaagcgccg gttacggaag gtgtttccgt aagaacaaac
     8161 aaccgtaatt ttgagggacg ttcgggaacc aagtctgccg gtatatatct ggtaagcccc
     8221 gaaacagccg cggttactgc aataaaaggc gtattgaccg atccttctac catgcctgaa
     8281 aacgaatacc caaaagtaac catacccgaa agttttatta tagacgactc gatggtgctt
     8341 aaacccgatt ttaaaggtga cgtgtttaga ggccccaaca taggagagcc gccattcaca
     8401 gaagaacttg ccgaaaaaat tattggaaag ataaacctga aagtcggaga caagataaca
     8461 accgaccata ttatgcctgc aggcgccagg cttaagtacc gctctaacgt acctgcatat
     8521 tcgcggtatg tatttgaagg ggtagatccg gaattttcct ctagagccat ggctgacaaa
     8581 gaaaaaggaa tttactcaat catcgtagct ggagcgagtt acggccaagg atcaagccgc
     8641 gaacacgctg ctatatgccc tatgtatctt ggagttcgtg ccgtggtagc aaaatcgttt
     8701 gagcgaattc actccgcgaa ccttattaac ttcggtattg taccttttat ttttaaaaat
     8761 gaagctgatt atgatacagt caaacagagt gatgatttta ttattgatgg aatccgcata
     8821 gcagtcgaga gcggctccga aacggcaact gcactttgcg gaaccaagaa gattgagctt
     8881 tcaatgactc taagcaaacg ccagagggat atacttttag ctggtgggct tttaaaccac
     8941 acaaaggctc aggcctgatt gagttttgac gaataacaaa agataaaaat ccaaaactgc
     9001 tgtattgcaa atatttccat ataattagaa atgagcggta ccaaatggtg ccgctcttta
     9061 tctatcatgt tactttacaa cagattgaat ctcctccaaa taaatttgat cgtctaagaa
     9121 atttcatgtt ttgacaatta tgcaacataa tatcatagac atcatacttt tctttaaaaa
     9181 agaaaaacaa aaaacgatat acaaatattt tcgcaaatat aaaaataagc tacttctttt
     9241 atcaacgaaa ataaacaaaa aaacattaag aggaagcaat gaaaccaaaa caatcagaac
     9301 gtcttgccta ttggtttgac aactacatgg ccaaagggcc gcgatctatt tttttcgctc
     9361 tgcttgttgt ctttgctgca gggtttatta cacttgccat tattcgtgta attgtaggct
     9421 tttatgtgat agaaggcaca accggagatg tacagaccga acttggagtg tggagaatct
     9481 tcttacagct aaccgatcca ggtaatatgg ctcaggataa tgactcgtac tggtttattc
     9541 gtattttcgc tgtaattagc ggtatgtttg gtattatttt cttttctgct gttatcgcct
     9601 ttattacaac acagcttgac cttaaactgg aagcattaaa gaaaggccga agtcgtgttc
     9661 tggaaaatgg ccatgttctt cttcttggtt ggagcaatga aattttcgat ataattcgtg
     9721 aacttattat tgcaaacgaa tctgaagcgt ctgccgcaat agttgtatta tccgatgtcg
     9781 agaaagaaga gatggacgat taccttaacg aacagataac tgaccgtaaa acaaccaaga
     9841 tcatcacaag aaccggtgaa gctggatcca tcaaggctct tgaaagagtt gccgtaacaa
     9901 aagccaaatc tgctataatt ttgcccacat gcacacatgg agccaccgat gccgagaagg
     9961 ctcattccga cgctcataca ttaaaaagca ccctggctgt agtagcagca tgccaaagca
    10021 aggaagagct tccttcgata gtaactcagg tttacaacga atctaaccgt gaaataataa
    10081 gaagccttgc tcccgacgac atcaccattg ttgacccgga agacatgatc gccaaaatta
    10141 ttgtgcaaac ctcgcgaagt agcgggctag ctgcggtata ctcgagtttg ataggatttg
    10201 acggttgtga attttatttt actaacgcac catggggcga attaccattc aaagatgtac
    10261 attttcacta ccccgatggt gtagcaatcg gagtctgcca taaagacgga tccataaaaa
    10321 ttaatcccga aaatgactac attcttaaaa aggatgacga tattattatt ttggctgaag
    10381 acgattccac tattcattac aagaatagat caatatgtac acctacaaaa attgaccttt
    10441 cagacaaacg aatcccttca gcaaaagaga aagtccttat tataggttgg aacaacaagg
    10501 ctccagttat aataagcgaa tttatggatt atattaccga tgactccgaa ataataatag
    10561 tgatcccgcc agatgttaca acagagagtg aagccttgtt gcaaataaag gccgacaatc
    10621 ctaattttgc gataaaaaca gtcaaggtgg atcttctgga tttacagcag cttgaagagc
    10681 tgtcgcccga atcatttaac agcataataa ttcttactgc aaatgaagat aatgatatcg
    10741 aacgggccga tgcggcgaca atcaatattc ttttgatgct acgcacagtc ataaggcgta
    10801 aagcggcaaa aggcaaagct ccacaaatta taaccgaggt tcttaactct gaaaacctcg
    10861 aactgatatc acaagccggg gttaacgatg ccgtaatatc tacaaaaatg gtttccaaaa
    10921 ttttatcaca aatagccgaa gaacccgaca tacttcaggt atacgaggag atgttcactg
    10981 aggatggaag cgaaatatat cttaaaccgc tagagctcta cttcaaaacc atgccatcta
    11041 cagtaaccta cgcggacctt atagctcttt cgcatcttcg tgaagagata tgtatcggat
    11101 acaggctaaa agagaacata atgagcgcca aggatgattt tggtgtgatt ttgaatccgt
    11161 caaaaactga aacaatcgaa gtagaaaaaa ttgactctct tatcgtaatt gccgaaaacg
    11221 aactatagta gaattacaaa cgaaaaggag cagtataaaa atatgctact ccttttcgta
    11281 atcttcgtag tatgatgtct cggtttcttc gttgtgaata atcgatacga ccctgattct
    11341 tccctcaaca gtatccgtat agaaggctga aggacgcggc tgatgaatat tgttcagttt
    11401 aatgtacttt ccctgtcgct cgtcaagaac gtattttaca acgctaatat gatgtcgatt
    11461 aatatctata gaggttgtaa aacttttttt ctgtgattcc agccctgtaa cagttctacc
    11521 cgattcgact ttttttatga aaactttata aaagcagcgc cgatcctgag cgggattttt
    11581 tttgtcagaa tagacttcaa ttttaaggtt atattctcca ggttttttat aacgattcgc
    11641 ctgtgtagaa gatgtctcga aaggtgccgg ccgatactcg gcatttcgag taatcgcatt
    11701 aatatcggca ccagcagaaa cgagcattct tattattatt ggattcgctc cagaaagagc
    11761 ataatgcaga gcagtaggtc cgaaagggct ctgacggtaa gaaagatcag gattatgctt
    11821 caacacagtt tgtaccatca ttgggctatc actctgaatg gcatacacaa gaagactttt
    11881 gccactgctg tcagaaagat taacgggtac tccctcctta agcagctctt caactacact
    11941 cgtgaacctc gcctcgacag gatccgacga gacaccaccc atagcggcaa ga
//
LOCUS       DAOTGZ010000068        11928 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_3064
            NODE_6386_length_11928_cov_5.221848, whole genome shotgun sequence.
ACCESSION   DAOTGZ010000068 DAOTGZ010000000
VERSION     DAOTGZ010000068.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868088
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 11928)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 11928)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:22:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,785
            CDSs (total)                      :: 2,751
            Genes (coding)                    :: 2,732
            CDSs (with protein)               :: 2,732
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 12 of 19
            Pseudo Genes (internal stop)      :: 5 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11928
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6386_length_11928_cov_5.221848"
                     /isolate="AMR_MDS_3064"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..2501)
                     /locus_tag="P5123_06145"
     CDS             complement(<1..2501)
                     /locus_tag="P5123_06145"
                     /inference="COORDINATES: protein motif:HMM:NF012742.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HRX15874.1"
                     /translation="MGNAVSSKPLKIGVMFPGLFSEYEKCIYHYSMNSVKERGHNFFA
                     LVSGLTQLFAKSFNIRKDALAKMFEGLDGAIVITSPLFNHTADIDKVTAFLDSITIPV
                     VSVGLPIPGKPSVIIDNNNGLSDAITHLAKFHKKKNIGYIMGNCNNPDSADRLRIYKD
                     VLNTLGLPSSDNYITTGDLCSEIPQEELATLLEQTNNRLDAIVCANDAIAAKVINEAK
                     DLNINIPEQIAIIGFDNSYDRGLTDIPISTIKQPLDQAMDEAVSLIEKAILDNKKSTH
                     LYLNTSFIARRSCGCFFPPDETNTTTDSIEKHDDIFNFIKNQLTYNTRKNIDDKLIND
                     WAAAYFKAREICTRDNCLSMINLFENMVYTCVRLGISSSQMFRVLISIINNGTDGLPL
                     NSVKSLFHHTGRIIEEMAIQTKIRERRTTHSHYKTLYAITEAFANITNNQELKHACME
                     AFHLAGISNFAIFLYSESNTDEVFFTSYATINGQPIEKDYTGHNLKSMYSAANFGKNC
                     PSVILPIFGEHHDLGYLIISDDRYDGIFYHILSILISNALKTVELIQTINNHTITLEQ
                     QITERTHELEDVNQQLQNLDSLKNDFIANITHDFKSPLSVVINMAELCLETGRYSDRN
                     FELIHQASLKLKTTVDRLLNLARMDAKGIELHLSDFDILKLVTRVSDFFRSTIIETGI
                     DICISADQKLSWMIHSDEEKIEDVINNIVSNAVKFVNPDNGKIIISLSQSLDQITIDI
                     RDNGIGIEPDMLDTIFRRFEQAEEGKKRRYRGTGIGLDFSKQLISFLKGKISAQSEGK
                     NKGACFTIEIPVDSQKNAVLEQADNRDVQETSTPA"
     gene            2751..3920
                     /locus_tag="P5123_06150"
     CDS             2751..3920
                     /locus_tag="P5123_06150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004082283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 5 protein"
                     /protein_id="HRX15875.1"
                     /translation="MNLKCKTLIFGLFLTLLIISACSGSSETDADNVLPEEYYTDMFD
                     WNSHKGLSDTKLGRGINLGNYLEAPRDSSDDGEGAWTDGRKLEREDIIRIADAGFAHI
                     RIPCRWTDYMSKTDPYDLIEINGYSRLERVKEIIGWAHEANLKVVINTHHYNEMFYSE
                     GSLTLEEHKERLNALWNRLSKEFKLADYPSENLIFELLNEPHENIGYDDWNEMIANLT
                     KIIWQDNASYQTNGDVKRIIMIGTANWGGVPGLYKLNLPDAINAENTIITVHYYEPFH
                     FTHQGAEWNEGASDWIGTRWLGSESERKPLIDLFEDIEKWNTKGFEIYVGEFGVYSKY
                     ADKTDQKAWTAFICRESERRNYSWAYWEYASGFGAYDPLNSTWRSWLKDALVPKD"
     gene            complement(4157..5350)
                     /gene="creD"
                     /locus_tag="P5123_06155"
     CDS             complement(4157..5350)
                     /gene="creD"
                     /locus_tag="P5123_06155"
                     /inference="COORDINATES: protein motif:HMM:NF017893.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell envelope integrity protein CreD"
                     /protein_id="HRX15876.1"
                     /translation="MKNQVIKPLVILGLIMGLLIPIQIVKSMVDERKERQRDVYNEIG
                     NRWGSPVTFGGIFIVSDNGIITAKSADIRCEMITETRRKGIFKVPCYNIDINIVASFE
                     PGTIAGEKSSVNFNLLHYGDVEINQLKINDKVNGLKNKTIEANRTRSSYELITLDTKD
                     KIETISAKFTMRGSESIAFIDQAENTTVQLSADWGDPNFTGKLPLKRDITQNWFSAKW
                     ISTSDTLLLKDTSISESDEDCFGVSLFVPISIYQQTDRILKYALLFIILTFSLFFAFE
                     HIYVLKIHPFQYLLVGVALTIFYMLLLALSEHIRFGLAYFSASAAVVLLVSLYCRSVL
                     NQQLRALLIALFLTVLYSFLFVLIQMQQFTLLVGSVGVFTLLAATMFFTRNINWYALH
                     EKIPS"
     gene            complement(5581..7011)
                     /gene="creC"
                     /locus_tag="P5123_06160"
     CDS             complement(5581..7011)
                     /gene="creC"
                     /locus_tag="P5123_06160"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: protein motif:HMM:NF008312.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     CreC"
                     /protein_id="HRX15877.1"
                     /translation="MNIKFRLVITFSIIAGLGFYYIFDSIYNDIRPRYFETTEESMND
                     TVNILAAYLENSADENGINLDSVRKLSINIYNRRFEAKIYSYRKTEVDLHFYICDKSG
                     HIIFDSDKGRRTGQDYSQWNDVYLALQGKYGARSTSTTPGTEGLLYVSAPLKHEGAII
                     GTVTVEKPKQSIISFIRLAQKKIIMLGIVSFVTFVVVSILLSFWITLPIKRLTHHVNS
                     LAHNRHSAIPKFSGSEIQTLASTFEQVFRELDGKKYIESYVNTLTHEIKSPLTSIKSA
                     AELLTEDMNDEQKHKFINNIQRESNRIQSLIDKMLLLSSLENRDELQKPEILCASEII
                     ADVTASLAPQAKNSEIDIIIDCPDKLYFSGEYFLIRHALLNLADNALKNSPLKSSITI
                     GCEKTNNNAVFTIRDHGNGIPDYAHKRLFEKFYSISSAQNKSKGTGLGLALVKECALL
                     HKGTIKIENCNDVGVIATLTIPINNS"
     gene            complement(7041..7688)
                     /gene="creB"
                     /locus_tag="P5123_06165"
     CDS             complement(7041..7688)
                     /gene="creB"
                     /locus_tag="P5123_06165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000226352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator CreB"
                     /protein_id="HRX15878.1"
                     /translation="MDNKKKILIIEDEPGIADSLEYVLKTEGFDTIWVQTGNEGLSIV
                     GDKQCDVVILDIGLPDINGFEVLKQIRHVSNIPVLCLTARAEEIDRILGLELGADDYV
                     SKPFSPREVSARIKAIMRRGNSNAITEDCPFIIDTNKRQITYFGQKLQLSRYEFDILS
                     LLANRPGWIFNRQKIMDLVWLEPDDSFDRTVDAHIKMLRAKLKAVRPDIDPIETY"
     gene            7826..8269
                     /locus_tag="P5123_06170"
     CDS             7826..8269
                     /locus_tag="P5123_06170"
                     /inference="COORDINATES: protein motif:HMM:NF024212.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family winged helix-turn-helix
                     transcriptional regulator"
                     /protein_id="HRX15879.1"
                     /translation="MHSKTNISFEVPERIVSLLSRKMRKWYHFIRIRIEEAGLSGLDI
                     SHGDILYQLYHHGAMPMNNIAQRIGRDKSTVTALVKKMESKNLIKRVSDIDDARITII
                     QLTSVGESYRDAFAKISHDLMERIYCNLTDKEKIKLMQLLAKCGE"
     gene            8324..8791
                     /locus_tag="P5123_06175"
     CDS             8324..8791
                     /locus_tag="P5123_06175"
                     /inference="COORDINATES: protein motif:HMM:NF039500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5680 domain-containing protein"
                     /protein_id="HRX15880.1"
                     /translation="MEKQFVEFLFKAKRNTYAVGENGVKEPQSDGSTIMRYQNKQLVY
                     VDRFYGGEPYIGEEILFYDNMAVWGMNYTGRLIEANWNLRGDVYAFLQMALKENGAAF
                     PARGPEIYRDADYTYRTNIKGTVEFFSGYEEVLFRGEVIYKGYYHGGSVNTNN"
     gene            8804..9301
                     /locus_tag="P5123_06180"
     CDS             8804..9301
                     /locus_tag="P5123_06180"
                     /EC_number="1.-.-.-"
                     /EC_number="1.4.3.5"
                     /inference="COORDINATES: protein motif:HMM:NF013412.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004733 - pyridoxamine-phosphate oxidase
                     activity [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_process: GO:0008615 - pyridoxine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxamine 5'-phosphate oxidase family
                     protein"
                     /protein_id="HRX15881.1"
                     /translation="MKSENKDSSINFNDAVDYVLKKLQKNKIAVLASCGADMRVSARS
                     MSIVNRGLEIWFQTDSRFKKNRQMDENPFVALCLENIQIEGRVEKTGHSLDPENKFFC
                     DEFELHHPGSYKAYTLSRDEVIYRVIAEQITLWRYDKGQSYRDFIDVQNRLAVREYYV
                     VEPLS"
     gene            9478..11103
                     /locus_tag="P5123_06185"
     CDS             9478..11103
                     /locus_tag="P5123_06185"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRX15882.1"
                     /translation="MQRCKDAKMQRCKDAKMQRCKVVMVILIFLALGFVSCEGEIHDA
                     RENEPAQGEKDVVKVVDGIVPIRKKEVSVTLNSLLSDNQDSDLRLRLSASRSTESNAD
                     NLISDLRELVLQSLEQMSEEEKKEFLNKDEISFTISSGIDPFEKIVYPIDPEDISAIS
                     EESEESEESEESEESEESEESEELNTLKSSKIQRRSSENGDISFIVDPYLARLQRRGQ
                     LKDYFEEICGNIQISGDILHNYYEKEIENIEEETDVGEQLIVPSLLYLSEVMFLLAEL
                     DQYNGIEELATNYDLNVDITRIKKDMEYLRSESDNETSGVAATRGSIPKDLNWKRIPN
                     GAILAKHSSGKLAAWISYIAGGVSELFSGGAKYNHAGLYNEALGKKGPGVKSVYSADG
                     DGTKVQSLNNFIKSSKFAIVYPTNWTSSKAKNAIKYAENIFGFPKEGKVKYFTKGQLT
                     KDYFRFSNRSHNLKGDRAICTKVPYTAWRKAGTSKKLDSGLGYSGFDRGGNLITPDEL
                     RNSAKIVTRKIDLWIWSHTTTVYTNRSYLFYEK"
     gene            11229..>11928
                     /locus_tag="P5123_06190"
     CDS             11229..>11928
                     /locus_tag="P5123_06190"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRX15883.1"
                     /translation="MQNKNIIIALLSATLLFVSCGIDPFAEDDVPLVYETSYKPVPYT
                     ETSVVLDIMGAGTNGEDNYLVVVNTEAAEVIGSYYFKEKDIGYIQYIQINEKLYMNFN
                     NDCYGDFVELDMITGKLIKLPFFDSYGYGISSLNDALYMGSGYGHNYNKLREYNTQTG
                     NYTDIDLGQNLSQWVYRGFVNDGNSYLVTGHYGQCFTDVLNNKTFDINDVLNNGYLCE
                     LYYIDLSLNPKNNVD"
BASE COUNT         3771 a         1963 c         2482 g         3712 t
ORIGIN      
        1 ttagcggggg tggatgtctc ttggacgtct cggttgtcag cttgctcgag aactgcgttc
       61 ttttgtgagt ccacaggaat ttcgattgta aagcatgctc ctttgttctt gccctcggat
      121 tgagcactta tctttccttt taaaaatgag attagctgtt ttgaaaagtc cagtccgata
      181 cctgttcccc gatatcgtct ttttttgcct tcctcggctt gttcgaatct tcggaatatt
      241 gtatcaagca tgtcaggttc gattccgatg ccgttatcgc gtatatcaat ggttatctgg
      301 tctaatgatt gggaaagcga tatgattatt ttaccgttat ctggattaac aaatttgact
      361 gcgtttgata cgatgttatt tatcacatct tcgatttttt cttcgtcact atgaatcatc
      421 cagcttaatt tttgatcggc cgatatacaa atgtcgatac cagtttcaat aatggtcgag
      481 cgaaaaaaat cggaaacacg ggttaccagt ttgagaatgt caaaatcact tagatgcagt
      541 tcaatacctt ttgcatccat ccgagccagg ttcaacaatc ggtctactgt tgtttttaat
      601 ttaagggaag cctgatgaat tagttcaaaa ttgcggtcgc tgtagcgtcc tgtttccaga
      661 cagagctctg ccatgttaat aacaaccgat agaggtgatt tgaagtcatg tgtgatattg
      721 gcgataaagt catttttgag cgagtcaaga ttttgtaact gctgattgac atcttcaagt
      781 tcatgtgttc tctctgtaat ttgttgttcg agagttattg tgtgattatt aatggtttgt
      841 atcagttcaa ccgtttttaa cgcatttgat attagtatcg aaaggatatg ataaaaaatt
      901 ccatcataac ggtcgtcact aatgatcagg tatccaagat cgtgatgttc tccaaaaata
      961 ggaagtatta ctgaagggca atttttgccg aaatttgcag cggaatacat ggatttcaaa
     1021 ttatgacctg tataatcttt ttctatgggt tgcccgttta tggttgcgta cgaggtaaaa
     1081 aacacctcat cagtgttaga ttcggaatag aggaatattg caaaatttga tatgccggca
     1141 agatgaaaag cttccatgca ggcgtgttta agctcttggt tgttggtgat atttgcgaaa
     1201 gcttcggtta tagcgtaaag tgttttatag tgcgagtgtg tggttcgtct ttctcgaatc
     1261 tttgtttgaa ttgccatctc ttcgattata cgcccggtgt gatggaaaag gctttttacc
     1321 gaatttaaag gaagaccgtc agtaccgttg tttataatgc taattaacac tctgaacatc
     1381 tgtgatgaac ttatcccaag gcggacacat gtataaacca tattctcaaa aagattaatc
     1441 atacttaagc agttatcgcg agtacaaatt tctcgtgctt taaaataagc agctgcccag
     1501 tcgttgatta atttatcgtc gatatttttt cgtgtattat atgttagttg atttttaatg
     1561 aaattaaata tatcatcatg tttttcaatt gaatctgttg tagtgtttgt ttcgtcgggt
     1621 ggaaagaagc acccgcaact tcttcttgca ataaaagaag tgtttaggta cagatgagta
     1681 ctcttcttat tatccaaaat ggctttttcg ataagggaaa ctgcttcatc catggcttga
     1741 tcaagaggct gttttattgt actaataggt atatcggtta gtccgcggtc gtacgagttg
     1801 tcaaagccaa ttatggctat ttgttcagga atattaatat taagatcttt cgcttcgtta
     1861 ataacttttg cggcaattgc gtcatttgca catactattg catctagtct attgtttgtt
     1921 tgttcaagaa gagtcgctag ctcttcttgt ggaatttcgc tacaaagatc acctgtagta
     1981 atgtagttgt ctgacgatgg taatcctaag gtattaagaa catctttata aattcttagt
     2041 ctgtcggctg aatcaggatt attgcaattg cccatgatat aaccgatgtt tttcttttta
     2101 tgaaatttgg caagatgagt tatggcgtct gacaaaccat tgttgttgtc aattataacg
     2161 gagggtttac cgggtattgg aagccctacc gatacaacag gaattgtaat gctatcaagg
     2221 aaagctgtta ctttgtctat gtcggcagta tgattaaaca gtggagaagt tataacaatt
     2281 gctccatcta gcccttcaaa cattttggcc aaagcgtctt ttcttatgtt aaagctttta
     2341 gcaaataatt gggtaagacc ggaaaccaga gcgaaaaagt tgtgtcctcg ttctttgacc
     2401 gaattcatgg aatagtgata gatgcacttt tcgtattcag agaagagtcc gggaaacatg
     2461 acgcctattt ttagcggttt tgaagaaacg gcattgccca ttaactttag taccttgaaa
     2521 tgtgatttat gaatcaaaca tcaagtgtta acttatcaca ttagacataa aaatacaaac
     2581 aaaaatccgc aaatgttaag gataaattct aaaagttttg tttgcaatta aaacatttgt
     2641 ttttgacaga ttatatgcta cattcatgtg agaagagagg aaaattttat tgtagacgat
     2701 cttggctgtg tgaaattcac ttcggaatat taaacaacag gggggataaa atgaatttaa
     2761 aatgtaaaac tcttattttt ggactgtttt taactctatt aattataagc gcctgttctg
     2821 gcagtagtga aacagatgct gataacgttt tacccgaaga atattatact gatatgttcg
     2881 attggaacag tcataaggga cttagcgata ctaagcttgg gcgagggata aaccttggaa
     2941 actatctgga agcaccacgt gacagttctg acgatggaga gggagcctgg acggatgggc
     3001 gtaagctaga acgtgaagat attattcgca ttgcagacgc gggattcgcg catattagga
     3061 taccttgccg ctggaccgat tatatgtcta aaactgatcc atatgatctt atagaaatta
     3121 atggttatag tcgtttggag cgtgttaaag aaattattgg ctgggctcat gaagcgaatc
     3181 tgaaagttgt aattaatact caccactata atgagatgtt ctattcggag ggaagtctta
     3241 cacttgaaga acataaagag cgtcttaatg cattatggaa tcgcctctca aaggagttta
     3301 aactggccga ttatccttca gaaaacttga tatttgaact gcttaatgaa ccgcatgaaa
     3361 atattggata tgatgactgg aatgaaatga ttgcaaactt gacaaagata atatggcagg
     3421 ataatgcttc ttatcaaaca aatggtgatg ttaaacgtat tataatgatt ggaacagcaa
     3481 actggggtgg tgttcccgga ctttataaat tgaacctgcc tgatgcaata aatgctgaaa
     3541 atactataat cactgtccat tattatgagc ccttccactt tacgcatcag ggtgccgaat
     3601 ggaacgaggg ggcttcggac tggataggta cgcgatggct tggaagcgaa agtgaacgta
     3661 agcctcttat tgatctgttt gaggatatcg aaaagtggaa tacaaaaggt tttgaaattt
     3721 atgtcggaga attcggtgtc tattcaaagt atgcggataa aactgatcag aaagcctgga
     3781 ctgcttttat ttgccgtgag tctgaacgtc gaaattattc ttgggcatac tgggaatacg
     3841 cctcggggtt tggagcttat gatcctctta acagcacttg gcgttcatgg cttaaggatg
     3901 cattggtacc aaaagattag ttgaacaatg attaaatagg gaaggtgatg ttttagaaga
     3961 taaaaatggt gactgtgacg ttttcaaata atggagacga tgcaaaaaaa tggggacggt
     4021 gaggtttaaa caaaaacaac tccgtccccg attcgcagaa gcaccaacgt accctatcaa
     4081 tgagacatcc agatgaatgc cttttttgat agggtattgc aaaaattaca cccctgtgga
     4141 atgtggcgaa tgatttttat gatggaattt tttcatgaag tgcataccag ttaatattac
     4201 gggtaaagaa catagttgcg gcaagtagag taaatacacc aacagaaccg acaagcagag
     4261 taaattgttg catctgaata agcacaaaca gaaacgaata gagaactgtt aaaaatagtg
     4321 ctatcagtag cgctcgcaac tgctggttaa gaaccgaccg gcagtataaa gagactagca
     4381 atacaaccgc ggcggaggcc gaaaaatacg ccaatccgaa tctgatatgt tctgacagag
     4441 caagtaataa catgtaaaaa atggtaagtg ctacaccgac aagaagatat tggaaaggat
     4501 gaatctttaa aacatagatg tgctcaaaag caaaaaatag ggaaaatgtc aaaattatga
     4561 aaagcaaggc atattttaat atacgatctg tttgttgata aatgcttatt ggaacaaaca
     4621 gtgatacccc aaaacaatct tcatcggatt cactgatgct ggtatcctta agaagtagag
     4681 tatcggaagt ggatatccat tttgcagaaa accaattttg tgttatatct ctttttaatg
     4741 gcaactttcc agtaaagttt ggatcacccc aatcggcaga caattgaact gtagtatttt
     4801 cggcctgatc aataaaggct attgactcac ttcctcgcat tgtaaacttg gcagaaattg
     4861 tttcgatttt atctttagta tcaagagtga ttagttcgta actggatcgt gtgcgatttg
     4921 cttcaatggt tttatttttg agtccgttaa ctttgtcatt gatttttaac tgatttatct
     4981 caacatcacc ataatgtaga aggttaaaat tgacagagct cttttcccca gctattgtgc
     5041 cgggttcaaa tgatgcaaca atattgatgt ctatgttgta acagggaact ttaaaaatac
     5101 cttttcgtct agtttcggtt atcatttcgc atcgaatatc agctgatttt gctgtaatta
     5161 tcccattgtc cgaaacgata aaaattcctc caaaggtaac cggacttccc catctgttgc
     5221 ctatctcgtt gtaaacatct cgctgccgct ctttacgttc atccaccata gatttaacaa
     5281 tttggattgg gatcaaaaga cccataatta gccctaaaat tacaagtggc ttaataacct
     5341 gatttttcat aatcgttctc catataaatt tgtgagcgaa atttttaaac taattaattc
     5401 gcttatataa ttaatctggt gtgcgattgt gaaaagagta tgaaggctgt gtgaagtgaa
     5461 taagaaatca taaaaaaagt gatagtacat ttgcagtctt tgatgtattt ctcacaattt
     5521 aagatgtttg taatatgacc attgcttttt ggacaaataa aatattaaaa agttattaca
     5581 tcagctgtta ttaataggga tagtgagagt ggcaataact ccgacatcgt tacaattttc
     5641 aatttttatt gtacctttgt gaagtaacgc gcactctttt accaaggcta gccctaaacc
     5701 agtgcctttg ctcttgtttt gggctgatga tatcgaataa aatttttcaa aaagtctttt
     5761 gtgagcataa tctggaattc catttccatg atcacggata gtaaaaacgg cattattgtt
     5821 tgttttttca cagcctatag ttatagatga ctttaaagga gaatttttta gagcattatc
     5881 tgcaagatta agtagggcat gtctgatcag gaagtattca ccactgaaat ataatttatc
     5941 gggacaatct ataataatat ctatttcgct gttttttgct tgaggagcaa gagacgcagt
     6001 tacgtctgct ataatctcgc ttgcacagag tatttcgggt ttttgtagct catcacggtt
     6061 ttcaagagac gaaagcaaaa gcatcttatc gataagcgat tgtattcgat tgctctcacg
     6121 ttgtatatta ttgatgaact tatgtttttg ttcatcattc atatcttctg ttaaaagttc
     6181 cgctgcactt tttatggatg ttaaaggtga tttaatttca tgcgtgaggg tgttgacata
     6241 ggattctata tatttttttc cgtccagttc acgaaagacc tgctcaaagg tagatgcaag
     6301 tgtttggatt tcgctcccgg aaaatttagg aatcgcagag tgcctgttat gtgcaagaga
     6361 gtttacatga tgtgtaagcc ttttaatagg tagagttatc caaaatgata aaagaatgct
     6421 caccactaca aaagtaacaa aacttacaat accaagcatg attattttct tttgggcaag
     6481 acggataaag ctgattatac tctgcttggg cttctcgaca gttacagttc cgattatggc
     6541 accttcatgt tttagtggag cagatacata cagcagtcct tcggttccag gtgttgtcga
     6601 ggtcgaacgc gctccgtatt tcccctgaag tgccaggtat acatcattcc actgagaata
     6661 gtcttgtcct gttcgccttc ctttatccga gtcaaaaata atgtgtccgg atttgtcaca
     6721 gatataaaaa tgcagatcaa cttctgtttt tcgatatgaa tatattttag cttcgaatcg
     6781 acggttatat atatttatag atagtttcct aacgctatca aggttgatcc cgttctcatc
     6841 ggcactgttc tcaaggtaag ccgccaagat atttacggtg tcgttcatgg attcttcggt
     6901 ggtttcaaaa taacgcggtc taatatcgtt ataaatagag tcaaaaatat agtaaaaacc
     6961 aagcccagct ataattgaaa atgtaataac aaggcggaat tttatattca taatccctct
     7021 tttagggagt aaccgatgcc tcagtaggtc tcgatcggat ctatatcggg gcgcacagct
     7081 ttcagtttgg cgcggagcat ttttatatgg gcatctactg ttctgtcaaa gctgtcatca
     7141 ggctcaagcc aaacaagatc cataatcttt tgccggttaa agatccagcc tgggcgatta
     7201 gccagcaaag ataaaatatc gaattcgtag cgagaaagtt gaagtttttg tccgaaatag
     7261 gttatctgtc ttttgttagt atcaattata aatgggcaat cttcggttat tgcatttgag
     7321 tttccgcgcc tcataattgc tttgatcctt gcactcacct ctctggggct aaaaggttta
     7381 gagacataat catccgcgcc aagttcaaga cccagaatgc ggtcaatctc ttccgcacga
     7441 gcggttaagc ataatacagg gatattagaa acatgacgaa tctgttttag aacttcaaat
     7501 ccgtttatat caggcaaacc aatatcgagt attacaacat cgcactgctt gtcgccgaca
     7561 atggaaagac cttcattgcc agtttgtacc caaatggtat caaaaccttc agttttaaga
     7621 acgtattcca gagaatcggc tatacctggt tcgtcttcaa taatcaaaat tttcttttta
     7681 ttgtccataa acgtatagtc accttttaaa ccaaaaaatg tccatgattt tttgccaccg
     7741 tccccgatta ccagaaacgt caccgtcccc aacgaaatat aaataatttg ttgacaaagg
     7801 tttgatgtca aactaaaatg aaattatgca tagcaaaaca aacatatcgt ttgaagtacc
     7861 agaaagaatt gtttcacttt taagtcgaaa aatgcgcaaa tggtaccatt ttattcgaat
     7921 acgtatcgag gaggcagggc taagtggact cgatataagc catggcgata ttctgtatca
     7981 actgtatcat catggagcga tgccaatgaa taatattgcg caacgcattg gccgggataa
     8041 atcaacagtt acagctcttg taaaaaagat ggaatctaaa aacttgataa aaagggtttc
     8101 agatattgat gacgcaagaa ttacgataat ccagctgact tcagtcggag aatcatacag
     8161 ggatgcgttt gcaaaaattt cgcatgattt aatggagcgc atatattgta atctgacaga
     8221 taaagaaaaa atcaaattga tgcagttact ggcaaagtgt ggagagtaaa aatttttaaa
     8281 tatatagttt gatatcaaac aaaagacaaa ggtaaaggta aagatggaga aacagtttgt
     8341 tgaatttcta tttaaggcaa agagaaatac ttatgctgtt ggtgaaaatg gtgtaaaaga
     8401 accacagtct gatggctcga cgataatgag gtatcagaac aagcaattgg tttatgtgga
     8461 tcgtttttac ggaggagaac catatatcgg cgaggaaatt ttgttttatg acaatatggc
     8521 agtatggggt atgaactata ctggcaggct tattgaagca aattggaatt tgcgcgggga
     8581 tgtgtatgcc tttcttcaga tggctcttaa agaaaatggc gctgcctttc ctgcgcgggg
     8641 tcctgaaatt taccgtgatg cagactatac atacagaaca aacataaaag gaacagttga
     8701 gttttttagt ggatacgaag aggtgctttt tcggggcgag gtaatataca aggggtatta
     8761 tcatggtggt tctgtgaata caaataacta aaaggagaca ataatgaaat ctgaaaacaa
     8821 agatagttca ataaatttta acgatgctgt tgattatgtt ttgaagaaac ttcaaaaaaa
     8881 caaaatcgct gtattggcaa gttgcggtgc cgatatgcgt gtcagtgcaa gatcaatgag
     8941 tattgtaaat agaggtcttg aaatatggtt tcagactgat agtagattta aaaagaacag
     9001 gcagatggac gagaacccct ttgtggcttt atgtcttgaa aacatccaga tcgaaggaag
     9061 ggtagaaaaa acagggcact ccttagaccc agagaataaa tttttctgtg atgagtttga
     9121 gttgcatcat cctggttcat ataaggctta tacacttagt cgtgacgagg taatatatcg
     9181 cgtaattgcg gagcagatta cactttggag atatgataaa ggtcagtctt atagggactt
     9241 cattgatgta caaaatagat tagcggttag agaatattat gtggtagaac ctttgagtta
     9301 ggaaaggctt atgttaagaa atagaaaaac tcagccttgt gggaaaaaca gatatgacaa
     9361 tatagattaa aatatacaca atagttgaaa tttatgatgt gagcaaaata aatattgata
     9421 taatctaaat ataatttaat tgtaatagtt gccttaaaat attaaggagg tcttattatg
     9481 caaagatgca aagatgcaaa gatgcaaaga tgcaaagatg caaagatgca aagatgcaag
     9541 gtagtaatgg ttatactgat ttttcttgca cttggatttg tttcatgtga aggcgaaata
     9601 catgatgctc gggaaaatga acctgcccaa ggtgaaaagg atgtcgtaaa agtcgttgac
     9661 ggtattgtcc caatccgtaa aaaagaggtt agtgtaactt taaactcgct tctttctgac
     9721 aaccaagatt ctgatttgcg tttacgtctt tcagcttcgc gaagtaccga aagtaatgct
     9781 gataatctta tttctgatct tcgtgaactc gttttgcaga gcttggaaca aatgtcggaa
     9841 gaagaaaaaa aggagtttct taataaagat gaaatttctt tcacaataag ttcagggata
     9901 gacccgtttg aaaaaattgt ttacccgata gatccagagg atatttctgc tatatccgaa
     9961 gaatccgaag aatccgaaga atccgaagaa tccgaagaat ccgaagaatc tgaagaatct
    10021 gaagaattaa ataccctgaa gtcatcaaaa atacaacgtc gatcctcaga aaatggtgat
    10081 attagtttca tcgttgatcc ttaccttgcc cgtttacagc gaagaggtca gcttaaagat
    10141 tatttcgaag aaatttgtgg aaatatacaa atttcaggtg atattcttca taactattat
    10201 gaaaaagaaa tcgaaaatat cgaagaagaa acggacgtag gggaacaact tattgtgccg
    10261 tctctacttt atctttctga ggttatgttt ctattggcag aactggatca atataatggc
    10321 atcgaagaat tggcaacaaa ttatgaccta aatgttgata ttacaagaat aaaaaaagat
    10381 atggaatatt taagaagtga atctgacaat gaaacatcag gtgtagccgc aacccgggga
    10441 agcatcccaa aggatttgaa ctggaaacgc ataccaaacg gcgctatatt ggcaaaacat
    10501 agtagtggta aattggcggc atggatatca tatatagccg ggggagtaag cgaattattt
    10561 tctggtggag ccaaatataa tcatgctggt ttgtataacg aagcattagg gaaaaaaggt
    10621 ccaggcgtga aatctgtata ctcggcagat ggagatggta caaaagtgca gtcactgaat
    10681 aattttataa agtcttcaaa attcgctatt gtttacccta ctaattggac aagctcaaaa
    10741 gcaaaaaacg caataaaata cgctgaaaat atatttggtt ttcccaaaga aggaaaagta
    10801 aaatatttta ccaaagggca attgactaaa gactatttca gattttctaa tagaagtcat
    10861 aatctgaaag gtgatcgcgc tatttgtacc aaagttcctt acacagcctg gcgaaaggcc
    10921 ggcactagta aaaagctcga ttccggatta ggttattcag gttttgatcg tggtggtaac
    10981 cttataacac ctgatgaact tcgaaacagt gctaaaatag ttacacgtaa aattgatctt
    11041 tggatctggt cgcatactac aacagtatat acaaatcggt catatctgtt ttatgaaaaa
    11101 taactgtaac tactttatta ctagagacca tataagcaag ttcgactaaa aaatattgat
    11161 tttagtgtga aattagtatc aatccgcggc actaggtgcc gcggtcatta cagcggaaag
    11221 gaatgattat gcaaaataaa aatattataa ttgccctttt gtcggcaacc ctgctgtttg
    11281 tttcgtgtgg tattgatcct tttgcggaag acgatgttcc gcttgtttac gaaacaagct
    11341 acaaacctgt accctatacc gaaacaagtg tagtgcttga tattatggga gccgggacaa
    11401 acggagaaga taactatctg gttgttgtga ataccgaagc ggccgaagta atcggttcat
    11461 attatttcaa ggaaaaggat atagggtaca ttcagtatat tcagattaac gaaaaactgt
    11521 acatgaattt taacaatgat tgttatggcg attttgttga gcttgacatg atcactggaa
    11581 aacttataaa actgcctttt tttgatagtt acggttatgg catatcttct ttgaatgatg
    11641 ctctttatat gggcagtggc tatggtcata attataacaa gcttcgtgaa tataacactc
    11701 aaacaggtaa ctataccgat attgatcttg gtcagaattt atcccagtgg gtatacaggg
    11761 ggtttgttaa cgatggtaat tcttatcttg ttaccggcca ttatgggcaa tgtttcacgg
    11821 atgtgttgaa taacaagaca tttgatatta atgatgttct aaataatgga tatttatgtg
    11881 agttatatta tattgattta agtttgaatc caaaaaataa tgtcgatg
//
LOCUS       DAOTGZ010000069        11808 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_3064
            NODE_6462_length_11808_cov_4.500893, whole genome shotgun sequence.
ACCESSION   DAOTGZ010000069 DAOTGZ010000000
VERSION     DAOTGZ010000069.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868088
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 11808)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 11808)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:22:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,785
            CDSs (total)                      :: 2,751
            Genes (coding)                    :: 2,732
            CDSs (with protein)               :: 2,732
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 12 of 19
            Pseudo Genes (internal stop)      :: 5 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11808
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6462_length_11808_cov_4.500893"
                     /isolate="AMR_MDS_3064"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..476)
                     /locus_tag="P5123_06195"
     CDS             complement(<1..476)
                     /locus_tag="P5123_06195"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRX15884.1"
                     /translation="MKKILKVVLFTALALYGSSLFAGSIDYLGNQSAEYVRIYNRNGA
                     LEGADIAHYNPAATAFMAQGFHVQASNQFLLKNYEIKSDLPTVEVADGSFETTEPTLF
                     LPNVYAVYNGGKWAAFASFGVTAGGGTLVYDDGTPEYNAGLYQAGMAAYAETGNIA"
     gene            827..1111
                     /locus_tag="P5123_06200"
     CDS             827..1111
                     /locus_tag="P5123_06200"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRX15885.1"
                     /translation="MRKILSLKECTMQSDSTSRIYQITKETCDRFNSYYLDFVESVAI
                     LNEKYEEGATKGKELKEQIEYMKKKIDELIGFMKEISREMGDFVELADVD"
     gene            1153..1920
                     /locus_tag="P5123_06205"
     CDS             1153..1920
                     /locus_tag="P5123_06205"
                     /EC_number="2.3.1.78"
                     /inference="COORDINATES: protein motif:HMM:NF019403.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015019 - heparan-alpha-glucosaminide
                     N-acetyltransferase activity [Evidence IEA];
                     GO_process: GO:0007041 - lysosomal transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heparan-alpha-glucosaminide N-acetyltransferase"
                     /protein_id="HRX15886.1"
                     /translation="MKYIKFLLNIHSGKGRYIEIDALRAFAILLMVFYHFSWDLMYFG
                     YIEIDVYNGFWYWFARFIASLFIFTAGIGIAIAYGRCKDYNDFVKSSIKRGLVIFMWA
                     AIISLVSLFQFGSGYMIIFGILHLLGFSVIASAFAVRINRVIIIAVAVVTVFIGYKLF
                     HMASHDLYLVWAGVQQYGRAMPDYYPVFPYFAPALVGLAVGKTFYSNGMHPDLKFLKK
                     NNAVKLLATMGRFSLLIYLVHQPILMAFFSIVQFFRG"
     gene            complement(1983..2831)
                     /locus_tag="P5123_06210"
     CDS             complement(1983..2831)
                     /locus_tag="P5123_06210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010419457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellin"
                     /protein_id="HRX15887.1"
                     /translation="MIINHNISSVFAHRTLKFHDWNLSSDIEKLSSGMRINKAGDDAS
                     GLAVSEKMRSQIQGLRQAERNTEDGMSMIQTAEGYLQETHEIIQRIRVLAVQAANGIY
                     TPEDRQLIQVEISELVDEVDRIASQAEFNKMSLLTGSFARLRPTASMWFHMGPNMHQR
                     ERVFIETMTSAALGLRNPTVLTFISISTPGKANAVIGLADEALRLISKQRADLGAYYN
                     RLEHAAKGLMNAYENTQASESRIRDTDMAEQMSSFTRYQILTQSAVSMLAQANNKSQS
                     VLKLLQ"
     gene            complement(3001..3498)
                     /locus_tag="P5123_06215"
     CDS             complement(3001..3498)
                     /locus_tag="P5123_06215"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRX15888.1"
                     /translation="MEVTDFRTSIDVEISRLDKIEEYAQKYNMDISDVMNTVLDMYSK
                     KLAKNPVCNDTLNRYHERGTVYKSVHIRFSSISKRKLKDIANLYCYSLSYMLFLAMDF
                     FDEFMIGVKEFIRPCFHAAFVHTFFGFPYFINSWGMNFFGDQDHSSGQESEIFSVFLK
                     NFIFS"
     gene            3749..6076
                     /gene="amt"
                     /locus_tag="P5123_06220"
     CDS             3749..6076
                     /gene="amt"
                     /locus_tag="P5123_06220"
                     /inference="COORDINATES: protein motif:HMM:TIGR00836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008519 - ammonium transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0072488 - ammonium transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ammonium transporter"
                     /protein_id="HRX15889.1"
                     /translation="MKLCLLLIVFISFSLSASESAGVALASDVDILWVTISAALVFFM
                     QAGFLMLESGLVRSKNTINVAIKNLVDYIAGSIGFFVVGFGLMFGSAGLFTGTDLFFL
                     KNYNTPQEYTFFLFQIAFMGTAATIVSGAVAERIKFHSYMLMSVIISVLIYPIFGHWV
                     WGGGWLAQLGFIDFAGSTVVHSVGGWIGLAGAIVLGPRKDKYSASGKLNHIYGHNLPL
                     SVLGTLILWLGWFGFNGGSTLALTDSMPLIVVNTTLSACAGGTAALLLSWILNRFKRS
                     GIEDIINGVVGGLVAVTAGCHLVNPAAALVIGSVSGLIVVVNVRLMDNVFKIDDVVGA
                     FTVHALCGIWGTLCIPFLAANPTKGGSFVVQLIGVAVCAVWSFGLGLLFFYIFKMLKI
                     LRVSNEAEEMGLNIAEHGARTMWLDLMGAMNSVADNKGLPPAIDVELGTESGTIAAIF
                     NKIMENLHQIVNSVSDNAVTVNAISGELSNTTAFISDTIATESSKLQQVSGFLDGIKA
                     AFSKTNELAAELTSDTAKSSDLATLLHDRFVNLNKELHHSTKHTLDSHNFSVRGEEDL
                     KNTVERMRQIESSSQKVTEVARFLVDISEQLTLLSLNASIEAARSGTEGQGFSIIAHE
                     INNLSDTTLKHTKEAARYINEIGSTVSSGAAALSEAMDSFQTIRNEIDILKRIMKMLM
                     EESDNYRSIVHEMRSFFSGINSKIDMITGMVRERDNDISEVFNALWGINQAFEELQSH
                     SESVKGRSRDLYESSELMKQIVGSLSGNKECPING"
     gene            6228..6902
                     /locus_tag="P5123_06225"
     CDS             6228..6902
                     /locus_tag="P5123_06225"
                     /inference="COORDINATES: protein motif:HMM:NF024929.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M15 family metallopeptidase"
                     /protein_id="HRX15890.1"
                     /translation="MKNSVFPKIVCIFLFLSAAAGIAEESLVVDSAMTREQALKGVNP
                     DCPEWILSNQALVEVYYYGFDGKIHKGQLMADFRLADDLQSVFKIIYDTKFAIKQVKP
                     ISVYTWDDFKSMRANNSSAFNYRVVPFSKTLSKHAYGCAIDINPVQNPYFTKDSVYPE
                     GAVYDPSSPGTLAAKDKIVAEFKKRGWRWGGDWRNKDYQHFDKLLSKKAAAGNKKYYI
                     WPWSVK"
     gene            6939..8309
                     /locus_tag="P5123_06230"
     CDS             6939..8309
                     /locus_tag="P5123_06230"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0030246 - carbohydrate binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="discoidin domain-containing protein"
                     /protein_id="HRX15891.1"
                     /translation="MKKIAVISVVILSVLSCAKKKIIEPVDAVVENIYATSIKLPAAK
                     NSPYQLFDDNSKGFWVTSPGTGPDEGVFIQFEEPIFIKKVEVDAVAESFLSYRLYING
                     VETKDYESSQKSVDINKQVRTFYLRIIDNSVFEDDQSVSIKNISFYNDQDKKMILALP
                     GIETGTIKASSSLSPVESYSVDFLFDSRKDFGWADGDSESDGTGEYIVFDFDKEVTID
                     SLKIWNGYQRSDEHYKANERVEKFSFGITGEDASSYELHDYEIQKVDLKESLTGKSFT
                     LKINSVYAGSKYKDLVISELRFIQSGRPFVLDSGGLEKLQKKNLKAKASNELYKKLVD
                     RRFLLTISETDNYVNQSLILRSNGSFVLWFVSEETGVSRSRYADGNWQIETDGRVKIF
                     GRIHNIENYVNMDNEFDPYSGGSEEDGEPGKEDVDSIKIFHDYISLTTEYLKSDRGLF
                     KPFKLQ"
     gene            8328..9893
                     /locus_tag="P5123_06235"
     CDS             8328..9893
                     /locus_tag="P5123_06235"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRX15892.1"
                     /translation="MKRVIALIIFIATFIATMVLLCKSPYANGWDGYFYIVQVKSWFV
                     EGAMHSEDHSLVYALLIVVQYIFRDYVLSYKIASALIRAFFITALYFLSVKIIKETKP
                     EKIDRNDTSLYIKALFVCVVAAVSSSSLYFITQFPKNMLGFAFLLTMLYFLPSVYKSA
                     SNGQLSKNDIRNAVVCIILFICTFLTHRFSGGIAVIVLFLFVIALLLEKMKQIIPYLF
                     ISSVFFIIVLIAVSLAFPGTLHVYDLERFSGAFNSSFIFTVFEFLNIISYKRAGVLWS
                     VELLVYYLCSVLFIISYVRSLIKREKHKFLYFTGIFVIITFGVFPFYNFDPSGIGYRF
                     YLGAQLFVPLACLIFFVNMHFESKSFLPVLVIVMIALSMFLVINYDYKKYDPPYEKYH
                     GMSQKLIKRLGVTKNDYQLFIMHKALAEIFTYETGLDALPWAPGIKYEGARVLRLVYG
                     VEPKLYKRFLSNEELEKVFLLQGRYTYLDEDLFGIFCERLKEFAPDEFEKINNWRNPL
                     KVRPDYLMRGEKR"
     gene            9890..10699
                     /locus_tag="P5123_06240"
     CDS             9890..10699
                     /locus_tag="P5123_06240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012062666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanW family protein"
                     /protein_id="HRX15893.1"
                     /translation="MMRPVKRSRLRICIGKCYYTVRRYISWIIEYRLFANRKDYVDMP
                     YNVYEHSTPIYRELVGVNLELLKNKEKNLRIAIKNLDGVVIEPGQKFSYWRLIGKPSI
                     RKGYLPGMVLFYGNLTSGTGGGLCQLSNLIYWISLYTPLKVVERYRHSYDVFPDSNRT
                     QPFGSGATCVYPYRDLQLYNDTDNCFQLRLKIMNGRLYGMINSDKPLLISYMVYQKEH
                     CITHELWGGYVRHNRIFRKEFDVNGRQLDDYEVTQNHAVMMYEPFLEKHYD"
     gene            10758..11558
                     /gene="zupT"
                     /locus_tag="P5123_06245"
     CDS             10758..11558
                     /gene="zupT"
                     /locus_tag="P5123_06245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006486808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc transporter ZupT"
                     /protein_id="HRX15894.1"
                     /translation="MIDENFWFALGLTLFAGLSTGFGSLMALFSKDFKPRFLALSLGF
                     SAGVMIYVSLIEIFVKSRESLTAFYGERLGHWATVASFFGGIAVIAIIDKMIPSYENP
                     HELKSVKQGVDSENKKLLRMGMFSALAIAIHNFPEGLATFMAAIADPALGISIAVAIA
                     IHNIPEGVAVSVPILFATKNRKKAFTLSFLSGLAEPVGALVGYFILRQFFNDAAMGVV
                     FGAVAGIMVYISFDELLPTAEEYGEHHIAIGGLIAGMGVMAVSLLLLA"
     gene            11610..>11808
                     /locus_tag="P5123_06250"
     CDS             11610..>11808
                     /locus_tag="P5123_06250"
                     /EC_number="3.4.23.36"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004190 - aspartic-type endopeptidase
                     activity [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase II"
                     /protein_id="HRX15895.1"
                     /translation="MNLTNLKRNISMVSTVLIINLFFDRITKVLAVQFLKDGPSHHYL
                     YNTFVLKYAENSGAFLSMGADW"
BASE COUNT         3339 a         1896 c         2506 g         4067 t
ORIGIN      
        1 tatgctatgt tgcccgtttc tgcataagct gccatacctg cctgatacag acctgcgtta
       61 tattcaggtg taccatcatc ataaaccagt gttcctccgc cagcagttac tccaaaagag
      121 gcaaaggctg cccattttcc gccattataa acagcataaa catttggcaa aaacagagtt
      181 ggctctgttg tttcaaatga tccgtccgca acttctacag tgggtaaatc gcttttgatc
      241 tcatagtttt tgagtagaaa ctggtttgat gcctgaacat gaaatccttg tgccataaag
      301 gctgttgctg cgggattgta atgagcgata tctgctcctt cgagtgctcc atttcgattg
      361 tatatgcgta catattcggc actctgattg ccaagataat caatgcttcc ggcaaaaagt
      421 gatgaaccgt atagagcaag tgcggtaaaa agtactactt taagaatttt tttcataaac
      481 aagcctccgc gggattaacc cgttttaatg tttttgtgat ttgtgcaact atcttgaaaa
      541 aataatgcca taacaaaata tttagttaat ttatagagaa cttcgaaaaa taaccgtatt
      601 ttagaaggtg tcaccgttta agtgcctatt ctaaatgaat ttttcgcgag ttgcttgttt
      661 aaaaaattac ttattgagat ttcctatgta ttgccttttt aacaatattg tcaatactat
      721 caatatttat aaaaaaatca agaattttct tttgaataat cataatataa gtctttatag
      781 aaagaagtca aaagttaaaa tgaaaataac cgtaaggaga attccgatga gaaaaattct
      841 aagcttaaaa gagtgcacta tgcaaagtga ttctactagt cgtatttatc agataacaaa
      901 ggaaacttgc gaccggttta atagttatta tctggatttt gttgaatctg ttgcaattct
      961 taatgaaaaa tatgaagaag gtgcgaccaa agggaaagag cttaaagagc agatagaata
     1021 tatgaaaaaa aagatcgatg aattgattgg ttttatgaag gaaatttcca gagaaatggg
     1081 tgattttgtt gaattggcag atgtggattg atttgtgttg acatattgtt gtaggtgttt
     1141 ttttatttgg ctatgaaata tataaaattt ttgttaaaca tacattctgg gaaggggcgc
     1201 tatattgaaa tagacgcttt gcgagctttt gccattttat taatggtttt ttaccacttt
     1261 tcatgggatt tgatgtattt tggctatatc gaaattgatg tgtataacgg cttttggtac
     1321 tggtttgccc gttttatcgc atcactgttt atttttacag ccggaattgg aattgctatc
     1381 gcctatgggc gttgtaaaga ttataatgat tttgttaagt cttctattaa aaggggtctt
     1441 gtgattttta tgtgggctgc cattatttca ttggtatcct tgtttcagtt tggttccgga
     1501 tatatgataa ttttcggaat actacatttg ttggggtttt cagttatcgc ttcggccttt
     1561 gccgtgagaa taaatcgtgt aattattatt gccgttgccg tcgtcactgt ttttatcgga
     1621 tataaactgt ttcacatggc gtctcatgac ctttatcttg tatgggccgg agtacaacag
     1681 tatggcaggg caatgcctga ctattatccg gttttcccat atttcgcgcc ggcccttgtt
     1741 ggtcttgctg ttgggaaaac attttattct aatggtatgc atcctgacct gaaattttta
     1801 aagaaaaata atgctgtaaa actgttggct acaatgggta ggttttcgct tttgatatat
     1861 ctggtgcatc aacctattct aatggctttt ttttccattg ttcaattttt tagaggttaa
     1921 taaataatcc aagccgactg ggaggccggc ttgggtagtg gtgttgcgat gttggctcag
     1981 acttactgaa gcagttttaa tacagattgc gatttattgt ttgcctgggc aagcattgaa
     2041 accgctgact gtgtaagtat ctgatatctt gtgaatgatg acatctgctc ggccatgtct
     2101 gtatctctta ttctggactc tgatgcctgt gtgttttcgt aagcattcat gagtcctttg
     2161 gccgcgtgtt ccagacggtt gtaatatgct ccgagatcgg cacgctgttt agagataagc
     2221 cttagagcct catctgctaa accgataact gcgtttgctt tacctggtgt tgatatcgag
     2281 ataaatgtta gaacagttgg gttacgtaga cccagagccg cacttgtcat agtctcaata
     2341 aaaactcttt ctctctggtg catatttggt cccatatgaa accacattga tgctgttggg
     2401 cgaagtcgcg caaatgaacc tgtaagcaga ctcattttgt taaattcggc ctgagaagca
     2461 atacgatcga cctcatcaac cagctctgaa atttcaactt gaatcagctg acggtcttcc
     2521 ggtgtgtaga taccgttggc tgcttgtacc gcaagaaccc gtatcctctg aataatttca
     2581 tgagtttcct gaagataacc ctcggcagtc tgaatcatgg acataccatc ttctgtgttg
     2641 cgctctgcct gacgtaagcc ctggatctgt gatctcattt tctcagaaac cgcaagacct
     2701 gaagcatcgt ccccagcttt gttgatacgc ataccagatg aaagtttttc tatgtcgctt
     2761 gaaaggttcc agtcatgaaa cttgagcgta cggtgagcga aaacagaact gatattgtgg
     2821 ttaataatca ttacttttcc tccttgaaaa ttaagttatc atccgtgata acatgtaatg
     2881 ttttttaaga ttacattaca agatcggtcg ttagtccgaa ttgcgtaata ctattttttt
     2941 aaaaagttgc ggttttttgt tttattcgta gttttctttt tatgtttctt ggtatttaga
     3001 ttaagaaaaa ataaaatttt tcagaaaaac agagaaaatt tcggattctt gtccagaaga
     3061 atggtcttga tctccaaaaa agttcattcc ccagctgttt atgaagtatg gaaacccgaa
     3121 aaaagtgtgt acaaaagccg catgaaaaca ggggcgaata aactccttaa ccccaatcat
     3181 aaactcatca aagaaatcca ttgcaagaaa tagcatgtac gacaagctgt aacagtaaag
     3241 attagcgatg tctttcagtt tccttttaga aatgcttgaa aaccttatat gtactgattt
     3301 ataaactgta cctctttcat gataccggtt aagagtatca ttgcatactg gattcttagc
     3361 cagttttttc gaatacatat caagaacggt attcattaca tcggaaatat ccatattgta
     3421 tttctgcgcg tattcttcaa ttttatcaag tctacttatt tcaacatcga ttgatgttct
     3481 aaagtctgta acttccattt tgccgcctcc ataaatttat ctatatatgt atacttgatt
     3541 atgttcgatt ttgttaaaat ttttttaaaa aatttcaatt tgtttcctta tatcagcatt
     3601 aaaggctgtg ttcgctttta ttatgtatga gtcactgtta cataaatgtt aaatagtccg
     3661 tgaatattta cattcgggta taacgtatct tgacatatag gtcaatatcg tcttgtttga
     3721 ttttggccta tatgctagga gtcgttatat gaaactttgt ttgcttttga ttgtctttat
     3781 ttcattttca ttaagtgcgt ctgaatcagc gggtgttgct cttgcaagtg atgttgatat
     3841 tttatgggtt accatatcgg cggctcttgt gttttttatg caggctggat ttttgatgct
     3901 ggaatctggt cttgtaagat caaaaaatac gattaatgtt gcaataaaaa atcttgttga
     3961 ttatatagct gggtctatcg ggttttttgt ggtaggtttt ggcttgatgt ttggaagtgc
     4021 aggattgttt acaggtactg atttattttt ccttaagaat tataatactc cacaggagta
     4081 tacgttcttt cttttccaga tagcttttat gggaactgca gctaccattg tttcgggcgc
     4141 agtagcagag cgaatcaaat ttcattctta tatgcttatg tctgtaatta tatcggtatt
     4201 aatatatcca atttttgggc attgggtctg gggtggagga tggctggctc aattaggatt
     4261 tatagatttt gcgggtagta cagttgtaca ctctgttggc ggttggattg gtctggctgg
     4321 tgcgattgtt ttggggccgc gaaaagataa gtacagtgca tcaggaaaac ttaatcatat
     4381 ttatggacat aatctccctc tgtcagtttt aggcacactt attttatggc tgggctggtt
     4441 tggttttaac ggtggttcta ctcttgcctt gactgactcc atgcctctta ttgtcgtgaa
     4501 tacaactctt tctgcatgtg ctggtggaac agcagctttg ttattatcct ggattttaaa
     4561 cagatttaaa agatctggta ttgaagatat aattaacggt gtagtcggag gcctagttgc
     4621 agttacggct ggttgtcatc ttgttaatcc tgcggctgca cttgttattg gctctgttag
     4681 cggtttgatc gttgtagtta acgtcagact aatggataat gtgtttaaaa ttgatgatgt
     4741 agttggcgcc ttcactgtac atgctctttg tggtatttgg ggaactttat gcattccatt
     4801 tttggcagct aatccaacaa aaggtggttc gtttgttgta cagctcatag gtgttgctgt
     4861 ctgtgcggtt tggtcgtttg ggttaggact attgtttttc tatatattca agatgctcaa
     4921 aatactgcgt gtttcaaatg aggctgaaga aatggggttg aatattgcag agcatggagc
     4981 taggactatg tggcttgatc tcatgggtgc aatgaactca gtagctgata ataagggact
     5041 tcccccagcc attgatgtcg agcttggtac cgaatccggt accatagccg caatattcaa
     5101 caaaataatg gagaatcttc atcaaattgt aaattctgtt tcggataatg ctgtaactgt
     5161 taacgctata tcaggagaac tcagtaatac cacagctttt atcagtgaca ctattgctac
     5221 ggagtcgtct aagctgcaac aggtttcagg ctttttggat ggcataaagg cagctttttc
     5281 aaagacaaat gagcttgcag ccgaattgac atcagatact gctaaatcta gcgatcttgc
     5341 aacgctactt cacgatcgtt ttgtaaatct taacaaagag cttcaccatt caacgaagca
     5401 cactcttgac agccataact tttctgttcg tggagaagaa gatttgaaaa atactgtcga
     5461 gcgtatgcgg caaatagaaa gttcgtcaca aaaggttaca gaagttgcca ggtttcttgt
     5521 ggatatatct gagcaactga ctcttttatc actaaatgca agtattgagg ctgcacgatc
     5581 aggtactgaa gggcaggggt tttctataat tgctcacgaa attaataatt tgtcggatac
     5641 aactcttaag cataccaaag aagccgcacg ttacattaat gagattggat ctacggtgag
     5701 ttcgggagct gctgctcttt ccgaggcgat ggattccttt caaaccatac gtaacgagat
     5761 cgatattctt aaacgcatta tgaagatgct gatggaagag agtgataatt atcgttcaat
     5821 tgtgcatgag atgcgtagct tcttttcagg tattaattcc aaaattgata tgattactgg
     5881 catggttcgt gaacgggata atgatatatc agaagtattt aacgctttat gggggataaa
     5941 ccaggccttt gaagagctac agagtcattc agaatcggtc aaggggcgca gtcgggacct
     6001 ttatgagagc agcgagctta tgaagcagat agtcggatcg ttaagtggta acaaagaatg
     6061 cccgataaat ggttgacaca ataaatattt tcatataaga gttaaatcta tataaaatct
     6121 tcactaatct gttaacgaag aaagaaccaa atttgctgtt ttttaaggga tggggtttcc
     6181 tatctctctg tataattgca tatttggcta attaggtgat atatattatg aaaaattcag
     6241 tatttccaaa aattgtttgt atatttcttt tcctttccgc tgccgccggg atagcagaag
     6301 aatcgttggt tgtagattcc gctatgactc gtgagcaggc tttgaaaggt gttaatcctg
     6361 attgtcctga atggatacta tccaatcagg ctcttgttga ggtgtactat tacggttttg
     6421 atggtaagat tcacaagggg caactgatgg ctgatttcag gttagccgat gatctgcagt
     6481 cggtctttaa gattatttat gatacaaaat ttgcgattaa gcaggtaaag ccgatttctg
     6541 tatacacttg ggacgatttt aagtccatga gagcgaataa tagctctgcg tttaattata
     6601 gagttgttcc tttttcaaag actctttcaa agcatgctta tgggtgtgct atagatataa
     6661 atcctgtaca aaatccttat tttactaaag actctgttta tcctgaagga gcagtatatg
     6721 atccttcatc tccgggaaca ttagctgcaa aagataaaat tgtggcggag tttaaaaaac
     6781 gaggatggcg ctggggtggc gattggcgca ataaagatta ccaacatttt gataagttac
     6841 ttagcaaaaa agctgctgca ggtaataaaa agtattatat ttggccttgg tctgttaaat
     6901 agtctaagat ttcgccggta attgttgagg aaggtaatat gaaaaaaata gctgttatat
     6961 ctgttgttat tctgtccgtt ttgtcttgtg ccaaaaaaaa gattatcgag ccggttgatg
     7021 cagttgtaga aaatatttat gctacatcaa ttaaacttcc ggccgctaag aacagccctt
     7081 atcagctttt tgatgacaat tctaaaggat tttgggtgac atcgcctgga accggtccag
     7141 atgaaggcgt ttttattcaa tttgaagagc cgatatttat aaagaaagtt gaagtcgatg
     7201 cagttgctga atcattttta tcttatcgcc tttatataaa tggtgtagaa accaaagact
     7261 atgaatcttc acagaagagt gtggatataa acaaacaggt gaggactttt tatctaagaa
     7321 taatcgataa tagtgtattt gaagacgatc agagtgtatc tattaaaaat attagttttt
     7381 ataatgatca ggataaaaaa atgattctgg ctttgccagg gatagagacg ggtactataa
     7441 aggcgtcatc ctctctttct ccagttgaat cgtacagtgt tgactttctt tttgattcac
     7501 gcaaggattt tggatgggcg gatggagatt ctgaaagtga tgggacggga gaatatatcg
     7561 tttttgattt tgataaagaa gttacaattg actctttgaa aatatggaat ggttatcaga
     7621 gatcggatga acattataag gctaatgagc gggtagaaaa gttctctttt ggaataactg
     7681 gcgaagatgc ctcgtcttac gaattacatg attatgaaat tcagaaagtt gacctgaaag
     7741 aatctctaac tggtaaatct tttactctaa aaattaattc tgtatacgct ggctccaaat
     7801 acaaggatct cgtgataagc gagcttaggt ttatacaaag tgggcgtccg tttgttttag
     7861 atagtggtgg tttagaaaaa cttcagaaaa agaatctaaa agcaaaagcc agcaacgaac
     7921 tttataaaaa acttgttgat cgacggtttt tacttacaat atcagaaaca gataattatg
     7981 ttaatcagtc attgatatta cgatcaaacg gaagttttgt tctttggttt gtttctgaag
     8041 agactggtgt tagtcgttcg cgttatgcgg acggcaattg gcaaattgaa actgatggtc
     8101 gagtcaagat ctttgggcgc attcacaata tcgagaacta tgttaatatg gacaatgaat
     8161 ttgacccata tagtggtgga tcagaggaag atggtgaacc tggaaaagaa gatgtcgact
     8221 caataaaaat atttcacgat tatatctctt taactacgga atacttgaaa agtgataggg
     8281 gtttgtttaa accttttaaa ctacaataaa tcaaaacagg acttgatttg aaacgtgtca
     8341 ttgctctaat tatttttatc gcaaccttta tagcaacgat ggttcttctt tgcaagagtc
     8401 cctatgcgaa tggttgggat ggctattttt acatcgttca agttaaatct tggtttgtcg
     8461 agggagcgat gcactcggaa gatcattctc tcgtttatgc acttcttatt gtagttcagt
     8521 atatatttcg cgattatgtt ctttcataca aaatagcttc ggctcttata agagccttct
     8581 ttataacagc tctttatttt ctatccgtta aaattattaa agagacaaaa cctgaaaaaa
     8641 tagatcgaaa cgatacttct ctttacataa aagctctctt cgtctgtgta gttgctgctg
     8701 ttagtagctc atcgttatat tttattacac aatttcctaa aaacatgttg ggatttgctt
     8761 ttttgctgac aatgctctat tttttaccca gtgtatataa atctgccagc aatggacaat
     8821 taagcaaaaa tgatattagg aatgcagtag tttgtattat tttgtttata tgtacgtttc
     8881 tgactcacag attcagtggt ggaattgctg ttattgtgct gtttctattt gttattgcgc
     8941 ttttactgga aaagatgaaa caaattattc cttatctttt tatctcttct gtgtttttta
     9001 taatagtgtt aattgctgtt tcattagcat ttccaggaac actgcatgtt tatgatcttg
     9061 aacgtttttc cggtgctttt aatagcagtt ttatttttac ggtatttgaa tttttaaata
     9121 ttatttctta taagcgggct ggtgttctgt ggagtgtaga gcttttggtt tactatttat
     9181 gctctgtttt gtttataatt agttatgtaa gatctttgat aaaaagagag aaacataaat
     9241 ttctctattt cacaggaatt tttgtcatta ttactttcgg agttttccct ttttataatt
     9301 ttgatccatc gggaattggt taccgttttt accttggagc tcaacttttt gtgcctctgg
     9361 cttgtttgat tttctttgtg aatatgcatt ttgaatcaaa atcatttctg ccggttcttg
     9421 tcatcgtgat gatagccctt tctatgtttc ttgttataaa ttatgattac aaaaaatatg
     9481 atcctcccta tgaaaaatat catggaatgt cgcaaaaatt aataaaacgt ttaggtgtca
     9541 ctaaaaatga ttatcaactt ttcattatgc acaaggctct tgcggaaatc ttcacatacg
     9601 aaactggtct cgatgcgtta ccatgggctc caggtataaa atatgagggt gctcgggttc
     9661 ttcgtctggt ttatggggta gaaccaaaac tttataaacg gtttctttcc aatgaggagc
     9721 ttgagaaggt gtttctttta caaggacgct atacttatct tgatgaagat ctttttggaa
     9781 tattttgtga gcgattgaaa gaatttgctc cggatgaatt tgaaaaaata aataattggc
     9841 gtaatcctct taaagttcgc cctgattacc ttatgcgtgg ggaaaaaaga tgatgcgccc
     9901 agtgaaaaga agtcgtttgc ggatatgtat tggaaaatgc tattatacag taagacgata
     9961 tattagctgg ataattgaat atagattatt tgcaaataga aaagattatg tagatatgcc
    10021 atataatgtt tatgagcatt cgactccgat ttatcgcgag cttgtagggg ttaatctgga
    10081 attgctaaag aacaaggaaa agaacctgcg catagcgata aagaatcttg atggtgtggt
    10141 catagaaccc gggcaaaagt tttcttattg gcgattgatt ggaaaaccct ccatacgcaa
    10201 gggttattta cccggcatgg tactatttta tggtaattta acatcgggta ctgggggagg
    10261 gctttgtcag ctttcaaatc ttatttattg gataagtctg tatacaccac ttaaagttgt
    10321 agagcgttac cggcacagtt atgatgtttt tccggatagc aatagaactc agccgtttgg
    10381 aagtggtgca acctgcgtgt atccttacag ggatttgcag ctttataatg acacagacaa
    10441 ttgctttcaa ttacgtttga aaattatgaa cggaagactc tatggaatga ttaattcgga
    10501 taagccgtta ttaattagct atatggttta tcagaaagaa cattgtatta cacatgaact
    10561 ttggggcggt tatgtaaggc ataaccgtat ttttcgaaaa gagtttgacg ttaatggacg
    10621 gcaattagat gattacgaag ttactcaaaa ccatgctgta atgatgtatg agccttttct
    10681 agaaaaacat tacgactgaa gtcgatattg ggactgattg tgattaccga taataatttc
    10741 ataatggagg aattctaatg attgacgaaa atttttggtt cgcgttgggg ttgactcttt
    10801 ttgcaggtct ctctactggt ttcggaagtc tgatggctct attttctaag gattttaaac
    10861 cccgcttttt ggctttaagt ctgggctttt cggctggagt tatgatctat gtctcattga
    10921 tagagatttt tgttaaatca cgggaatcgt taactgcttt ttatggtgaa cgtttggggc
    10981 attgggcaac tgtcgcctct ttttttggag gaatcgcggt tattgctatt atagataaga
    11041 tgataccctc ttatgaaaat ccccatgaac taaaaagtgt taaacagggt gttgatagtg
    11101 agaataaaaa attattacgc atgggaatgt tttcggctct tgcaatagcc atacataact
    11161 tccctgaagg gttggcaact tttatggccg caattgctga tccagctttg ggtatcagta
    11221 tagctgttgc gatagcgata cacaatatac cggaaggggt tgctgtttcg gtgccgattc
    11281 tgtttgcgac aaaaaaccgt aaaaaagctt ttacgctttc gtttctttca ggtcttgctg
    11341 aaccggtagg tgcattagtc ggttatttta ttttgcgcca gttttttaac gacgcggcaa
    11401 tgggagtagt gtttggtgct gtagcaggta tcatggttta tatctctttt gatgagctat
    11461 tacctaccgc cgaggagtat ggtgaacacc atattgcgat aggtgggttg attgccggaa
    11521 tgggagttat ggcggtgagt ctgttattgt tggcttaaat tttgcgtggt tttgcgtggt
    11581 tctataattt taaataacgg aatagggaaa tgaatctgac taatttaaaa cgaaatattt
    11641 cgatggtttc gacagttctg attatcaatc ttttttttga tagaataaca aaggttttgg
    11701 ccgtgcagtt cttaaaagat gggccttcac atcattattt atacaataca tttgttctta
    11761 aatatgccga aaatagtgga gcttttttga gtatgggagc agattggc
//
LOCUS       DAOTGZ010000070        11804 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_3064
            NODE_6473_length_11804_cov_4.816495, whole genome shotgun sequence.
ACCESSION   DAOTGZ010000070 DAOTGZ010000000
VERSION     DAOTGZ010000070.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868088
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 11804)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 11804)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:22:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,785
            CDSs (total)                      :: 2,751
            Genes (coding)                    :: 2,732
            CDSs (with protein)               :: 2,732
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 12 of 19
            Pseudo Genes (internal stop)      :: 5 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11804
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6473_length_11804_cov_4.816495"
                     /isolate="AMR_MDS_3064"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..190)
                     /locus_tag="P5123_06255"
     CDS             complement(<1..190)
                     /locus_tag="P5123_06255"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRX15896.1"
                     /translation="MEVSDFRTSIDVEVSRLDRIEEYAAKHDMDISDVMNTVLHEYSK
                     KLAKNPVCNDTLNRYHERG"
     gene            complement(711..1598)
                     /gene="hslO"
                     /locus_tag="P5123_06260"
     CDS             complement(711..1598)
                     /gene="hslO"
                     /locus_tag="P5123_06260"
                     /inference="COORDINATES: protein motif:HMM:NF001033.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp33 family molecular chaperone HslO"
                     /protein_id="HRX15897.1"
                     /translation="MQDKFERFLCPALSLRAYTALTTNTAKEIATLHNTTPNATYAMS
                     EAITATALLSSLLKPDSRQSLSLKISGSGPMGTIYIQTDTTGALRGYAENPQPETKQK
                     FDSIDFSHAIGAGVITVTRDIGMKEPYSGVSPLIYGSIAKDTAYYLTTSEQIPSAVIL
                     TCDTDNDGYIISSGGILIQTLPDTPEESIAIVERSMQKSKTLKEHLGTGEDITSYLSY
                     LLENNTLELLSSTSLHYQCSCSKDIVMASLAAVSAKDLEVMIEEDHGAEVCCTFCNKA
                     YKFTESDLKDIIDKKEILQ"
     gene            complement(1624..3321)
                     /locus_tag="P5123_06265"
     CDS             complement(1624..3321)
                     /locus_tag="P5123_06265"
                     /inference="COORDINATES: protein motif:HMM:NF014075.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF115 domain-containing protein"
                     /protein_id="HRX15898.1"
                     /translation="MSSIKIIASKSGDITAEIISNGKNLRLHSAFDPVKEAFRSVSSF
                     TSSKNRLIIIAGLGLGYHIKAIKEKYPNNAILIIEKNQEMIDLCHKHCSDNVKNIRII
                     TNPDDAASALEKLDMASFKGTSVFFHRQSYGLDKDFYDMIITDMSRYLTSKISDLLTR
                     FEFEQKWAENILLNTSQLADSFPASLLFDRFKGIPGIIVSAGPSLIKSIDSLKKYKDH
                     ALIVAVDTAYKVLLKHDIEPHIVMVLDAQKQSQLHFFGANPGKTVLIADIVSSPAVIR
                     NFKNHKFISTTAKYYDDSGGKLKREATPFVDWLEKYIEPLGDIQSGGSVATSVFDFLL
                     NSGCSSIILVGQDLAYTGRKIHSNGTHHNEKWISLINRFNGLENINQAIIRKRKIKYV
                     DSIIKEEKIITDFVLDLYLSWFSDSAKKIKIPVYNATAIGANIPGTIITTLDEFFIGK
                     KSNKSDASEIIETITRRSQKFKIEKLKEAFSSTIKALEETAELLSNKSINADAVLDNM
                     EKNDLDVVYRPFMKKTLTYLKRNTLTDLKQNQMLLDELSHVTKKVTVLFQQGLDRLN"
     gene            complement(3318..4826)
                     /locus_tag="P5123_06270"
     CDS             complement(3318..4826)
                     /locus_tag="P5123_06270"
                     /inference="COORDINATES: protein motif:HMM:NF020252.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HDOD domain-containing protein"
                     /protein_id="HRX15899.1"
                     /translation="MALYALQHINLNDKRKEIINKVISGDQVKLSFSFPQKSYMLFLN
                     GMIANLLGHTDLIFLHESFFTILRESLLNAVKANAKRLYFEKHNADIHNSADYDRIMK
                     KFRNEVIGDMAVVEETLNRSRLYVNFIVELTKTHILLRIENNSKILPAEEERVQKRIT
                     QAWSFKDFSEAYDTMYDETEGAGLGIILTVMLLKNIGVTPDDYRIFAEKDMTIVSLRI
                     PLLIKKQETITAVKEKILREINLLPTFPQNIMDLQQLCDNPDSSINLIADKIQTDPSL
                     TADILKLANSAGFLTVRKIENVSEALMRIGLTNLKYILIAASSRKIMDKRYKQFSKIW
                     AHCLDVAAYGRFIVNKLSMRRLHDKVFISSLLHDLGKIVLLSVDLDLTNWIADFVQQR
                     GIRSTTILEEVSIGISHSTIGKLIAKKWNFADFMEEAIAFHHAPLQASEENRTVIYIT
                     YLANIFVGISTKKYTLDHIDPSVLAHLSIKSIKDCEDLYNYCTISLKESRDL"
     gene            complement(4948..6807)
                     /locus_tag="P5123_06275"
     CDS             complement(4948..6807)
                     /locus_tag="P5123_06275"
                     /inference="COORDINATES: protein motif:HMM:NF015714.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ComEC/Rec2 family competence protein"
                     /protein_id="HRX15900.1"
                     /translation="MELQSAQQQRWEIFKKLDSAIRLPSFILLVYITLLNLLLYWFYL
                     PETVKPFPIVLALTILTALVAYSVVLKTFKRLITISVLPLVIISLLLLTRFFIMIHPI
                     QNESEIISEQNFRHNCMVSFIYNGNKTAAFIDKDVRNREITVSGKPKEIRPDTDFNIF
                     LLRKGITHSAFVKTTKKQNASFRKKLRRKVDSSLIRMFPKEEYALIKALLFGDKSSLS
                     RKTLHLYKKAGLLHLLAASGMHVAIVSGSIFFLAGAISGKKSIALIMASTAVILYTFI
                     TELPVSLLRAAIMFWIYAFFVLLKKGKNAINALFLSGVFIQIIDPSDIFSLGFQLSFG
                     ATLGILLFYKDFLEIIPKIPLKLSQSIALSLSAQLSVIPLIFFSLGEFNPASMISTAT
                     ASPLLLFFIYSSFLKVFLFWLSFAIPYLSSINSFFYKALELNTIIFSKANLNCRPESF
                     IVLFLPSMALLGSLFLPSSKKIKKSLVITALLFMYTVPIIYNTDYFIYNNWSYNQSIL
                     PDKYGNTTVIYKETSAVQIANELNKRNIESVTLQISSITPKSWVSELIRLYPIKKAIF
                     VDILSKKDYLLFTKKLNQENIRYKTVLPDGYRHSRAKKKKRVSKKSKAGLLNN"
     gene            complement(6764..7498)
                     /locus_tag="P5123_06280"
     CDS             complement(6764..7498)
                     /locus_tag="P5123_06280"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRX15901.1"
                     /translation="MRYSVAVIILFITLAIFFPLFSQTDGTAADFDNYDPAAETTEIS
                     PETNNDDETQDAASTNDYPVIENKEPETFSGEDRNTRYYTYIGSTVSAGLNSISYSGW
                     ESGKQSSIEESGYYFFPAVCAFIIAPPLMGQFNLGFAINLNNSELTSINHVKADARIK
                     YMYKENLPVTLTGGAGFFIEMPPASEKYDGGGALITLGLLHPLSSDWSLLCDFDLGYG
                     YFGKGSEGRKLTYGITIGAAAKVGNL"
     gene            complement(7498..8079)
                     /locus_tag="P5123_06285"
     CDS             complement(7498..8079)
                     /locus_tag="P5123_06285"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRX15902.1"
                     /translation="MKRIIFILILLFALPLYSITGSEALSKFRARMRSIGSMRGMISM
                     STEGGMMNGTFSYKSPGKIHVQFDNGKTIVSNGRKLWVYNPESGICGVQDLGGGGSGG
                     IASLIDGYTAMAREGGSGAVIKLTGSGYYDDITIITDASYMLRSATFNNSSGGGFSVN
                     LSGVQLNLGLPGGMFDYSVPANAQLVNNPLNIQ"
     gene            8316..9113
                     /locus_tag="P5123_06290"
     CDS             8316..9113
                     /locus_tag="P5123_06290"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRX15903.1"
                     /translation="MKRKKKKKSGLKSAFFYLLVVIALIGGVFSAFYSDIVYKHYLLF
                     YYHIIRKDSFNIYYKELVELYKSEDSNDFLSYATRLSLIYPERKELKKIVAAHYFKIG
                     ENHAGFLPALQSLTYSSADIPLLERFVPALFAENMYSDLLSIGSKYRLDDIKGANGIV
                     LYYGAALYETGNYKKAVSVLSLLKGENSDPLYYLYLGKSCEKQALELSGSNRRSMFES
                     ALSHHKEGLNNYPGHKELYNAVVRVLKELKRDNEAAELIGEYRLRKQ"
     gene            9110..10135
                     /locus_tag="P5123_06295"
     CDS             9110..10135
                     /locus_tag="P5123_06295"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRX15904.1"
                     /translation="MRRFLLFTIMVLFFVTVMAEGILFDEPEKGALLLLEKYSNESYR
                     IAVMTSKNSYSFSGRKVYGDPFKTHLKGDSRYVFLSSLSVAIHESTHYYSEIYSLKRI
                     AKKSRIKSADDYFCYYESSGQGILVKVTNVFPSRDIKDIIDENLRGIYYKVYIDSNDS
                     SLVSQGMGIYGLLDEMNAYIRGARVVLDLYPLYIQNNPGEGLIRYQEDIVPDITAALD
                     FRYYIFAYLIHAQNNRPNIYKSIVNNRSFLTAMIKIDNDLHKLLSDYYQNKKEIEKQY
                     SKFSIKISESGSLMTIDFPDESRLRTVLNCEHYRLMNAKFDEASYQAIAKVLGVFEKL
                     PRYKSFK"
     gene            10290..10706
                     /locus_tag="P5123_06300"
     CDS             10290..10706
                     /locus_tag="P5123_06300"
                     /inference="COORDINATES: protein motif:HMM:NF036808.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRX15905.1"
                     /translation="MKSGKVVSLKKGREKSLLRRHPWVFSGAVKEFDKDISPGETVIV
                     TDYSGKFLAYGSYSPVSNITVRVWSFEESEQIDCYFFDRMINKTVSRKKALGFTCDNR
                     SAYRIIHGESDGLPGIIADYYAGFVCVQLLSCGAEF"
     gene            10732..11493
                     /locus_tag="P5123_06305"
     CDS             10732..11493
                     /locus_tag="P5123_06305"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF022137.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent rRNA methyltransferase"
                     /protein_id="HRX15906.1"
                     /translation="MNHTSCKGIYERSDTSARAKEGLLNSSGHLSGEIPPENLVIEEN
                     GVLLHIDIINGHKTGYYLDQRESRRKLSDYASGASVLNCFAYTGGFGHFACKGEASSV
                     THIEISDSAVKQIKKNAELNGFSDKRIRVLKGDVFKVMRSLLEQEQYFDIIVLDPPKF
                     ADSRFNIQKAARGYKDINMLAFKLLNPFGILFTFTCSGAVDELLFQKIVAGAALDAKR
                     DASIIDYLGQSADHPVDLAFPEGRYLKGLVCQCLQ"
     gene            11639..>11804
                     /locus_tag="P5123_06310"
     CDS             11639..>11804
                     /locus_tag="P5123_06310"
                     /inference="COORDINATES: protein motif:HMM:NF012442.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HU family DNA-binding protein"
                     /protein_id="HRX15907.1"
                     /translation="MNRQELVELLVKDKNAGFESKAAADRALKAVINGITEAIKKDGS
                     LQLVGFGTFSV"
BASE COUNT         3646 a         1943 c         2320 g         3895 t
ORIGIN      
        1 tacctctctc gtgatacctg ttaagagtgt cattgcatac tggattctta gccagttttt
       61 ttgagtactc gtgcaaaaca gtattcatta catcagaaat atccatatca tgctttgcag
      121 catactcttc aatcctatca agtctgctga cttcaacatc tattgatgtt ctaaaatctg
      181 aaacttccat ttcgtacctc caatttattt ctatatatgt atactgggtt gaaggctatt
      241 ttgttaaatt tttttggttt tttgaggtat gaaatctagt agctgcgcat taaatttgtg
      301 gaggcgttat tttcaaagca acaatctatt tgttctttct ttttttctct ttcagagggg
      361 ttttcttgtt tttaacaaaa atgttttgtg cgaatagccc gaaaatgttc catgcatatc
      421 accacttcgc ggagtgacag cgacgcgaag tggtgatatg ccacgagagg ctgcctgccg
      481 cccattgacc cttgcacgaa gaggattatg ataaaactat aagatgcaaa caactataga
      541 attcataaaa caagagagcg gccatttgtt ttaaatgcag tgttataata atatttaagt
      601 tttcagatgt aattgtttcc tgctttctcg gatttatcgg aaataggaat ttgttgttgt
      661 tttcatacag atgttactct ctggtaacat tggaattttt ctaagttcct ctattgtaat
      721 atctcttttt tgtctattat atctttaagg tcgctctctg tgaacttgta tgccttattg
      781 caaaaagtac agcatacttc ggcaccgtga tcttcttcaa tcattacttc caaatctttt
      841 gccgaaacag ctgctaaaga agccattact atatcctttg agcatgagca ctggtagtgt
      901 aaagatgttg aagaaagaag ttctagtgtg ttgttctcta gtagatatga gagatacgac
      961 gtaatatctt cacctgtacc gagatgctct tttaatgtct tggacttttg catggatcgc
     1021 tcgacaatcg caattgactc ttctggtgtg tcgggcaagg tttgtataag aatacctcca
     1081 ctggatatta tgtatccatc attatcggta tcgcatgtca ggattaccgc tgaaggtatc
     1141 tgttctgatg tagttaaata gtaggccgtg tctttggcta tagatccgta tataagagga
     1201 ctaacgccgc tataaggctc tttcatgcca atatccctgg taacggttat gactccggcg
     1261 ccaattgcat gggaaaaatc tatggaatca aatttttgct ttgtctcggg ttggggattt
     1321 tccgcgtatc ctcttagagc tccggttgta tccgtttgta tatatatggt tcccattgga
     1381 ccgctgcctg atatttttag cgatagtgat tgccgggagt ccggtttaag cagagatgaa
     1441 agaagagcgg ttgcggttat ggcttctgac attgcgtaag ttgcgttggg tgttgtatta
     1501 tgaagggtag cgatctcttt tgcggtattt gttgttaaag ctgtatatgc tcttagagaa
     1561 agagcagggc ataaaaagcg ttcaaattta tcttgcacgg tagtctccga agtaagttaa
     1621 catttaattt agcctgtcaa ggccttgttg aaagagaacg gttacttttt ttgtaacatg
     1681 gctcagttcg tcaagtaaca tttgattttg tttcagatca gtaagtgtgt ttcgtttaag
     1741 gtaggttagt gtttttttca taaatggtct gtaaacgaca tcaagatcgt ttttttccat
     1801 gttgtcaagt acggcgtctg cattgatact tttgttagaa agaagttcag cggtttcttc
     1861 taatgcctta atggtactac taaaagcctc tttcagtttt tctattttaa atttttggga
     1921 tcgtctggta atagtttcga taatctccga tgcatcagat ttatttgatt ttttaccaat
     1981 gaaaaattca tcaagggttg taataatggt accgggaata tttgcaccga ttgcggtagc
     2041 gttataaaca gggattttaa ttttttttgc agagtcgcta aaccatgaaa gatagagatc
     2101 aagaacaaag tctgtaatta ttttttcttc tttaatgatc gaatcgacat atttaatttt
     2161 acgcttacgg ataattgcct gattaatatt ttcaagtccg ttaaaccggt taataaggga
     2221 tatccacttt tcgttatgat gagttccgtt tgaatgaatt tttcgcccgg tataggcaag
     2281 atcttggccg acaagtataa tagatgagca tccgctattt aataagaagt caaatacgct
     2341 tgtggcaact gatccaccgg attgaatgtc tccaagcggt tcaatatatt tttctaacca
     2401 gtcaacaaag ggggttgctt ctcgtttaag tttaccgccg ctatcgtcgt aatattttgc
     2461 agtagtgcta ataaatttgt ggttcttaaa attacgtatt acagcaggcg agctgacaat
     2521 atctgcaata agaacagttt tgccagggtt tgctccaaaa aaatgtagct gactctgttt
     2581 ctgtgcgtca agaaccatca caatatgcgg ttctatatcg tgttttaaga gcactttgta
     2641 tgcagtatcg actgcgacga taagagcgtg atccttgtat tttttcagtg aatcgataga
     2701 cttgattagt gatggtcctg ctgatacgat aattccgggt attcctttaa aacggtcaaa
     2761 tagaagtgaa gctgggaaag aatcagccag ttgtgatgtg tttaatagaa tattttcggc
     2821 ccatttttgt tcaaattcaa aacgagttaa cagatcactt atcttagatg tcaaataacg
     2881 tgacatatct gttattatca tatcataaaa atctttatcc agcccataag attggcgatg
     2941 aaaaaaaaca gatgtacctt taaatgaagc catgtcaagt ttttcaagag cagaagccgc
     3001 atcatcaggg tttgtaatta tccttatatt cttaacattg tcagaacagt gtttgtgaca
     3061 aagatcgatc atctcttggt tcttttcgat tatcaaaata gcattgttag ggtatttttc
     3121 tttgatggct ttaatatggt aaccaagacc aagcccggca attattataa gacgattttt
     3181 agaagatgta aaagatgaga ctgatcggaa agcttcttta actgggtcaa aagcagagtg
     3241 cagtctgagg tttttgccgt ttgaaataat ttcggctgtt atatctccgg attttgacgc
     3301 aattattttg atactgctca tagatctcgg gattctttca atgaaattgt acagtagtta
     3361 taaagatctt cgcaatcctt gatcgactta atagagagat gggctaatac acttggatca
     3421 atatggtcaa gagtatattt ttttgtgctt ataccaacaa aaatattagc aagataggtt
     3481 atatatataa cagtacgatt ttcttcactt gcctgtaatg gcgcatggtg aaatgcgatc
     3541 gcttcttcca tgaagtccgc gaagttccat ttttttgcta taagtttgcc aatagtcgaa
     3601 tggcttattc caatagaaac ctcttcgagt atcgttgtgc ttctaattcc tctttgttgt
     3661 acaaaatcag caatccagtt tgtaagatca agatcaaccg ataaaagtac aattttacca
     3721 agatcgtgca aaagagatga aataaaaact ttatcatgaa gacggcgcat ggagagtttg
     3781 ttaacaataa accggccata ggctgcgaca tcaagacaat gtgcccatat cttggaaaat
     3841 tgtttatagc gcttatccat tattttgcgt gagcttgccg caataagtat atatttgaga
     3901 ttagttaatc caattctcat aagagcttca ctgacatttt caattttacg aacagttaaa
     3961 aatccggcag agttggctaa tttaagtatg tcggcagtta aggaagggtc agtttgtatt
     4021 ttatcggcaa taagatttat tgaggagtcg ggattatcac aaagttgctg aagatccata
     4081 atattctgag gaaaagttgg aagtagattg atttcgcgca gaatcttttc tttgacagcg
     4141 gttatggttt cctgcttttt aatcagaaga ggaatacgaa gagatacaat agtcatatcc
     4201 ttttcagcaa agatacggta atcgtccgga gtaacaccaa tattttttaa aagcataacc
     4261 gttaatatta tgcccaaacc ggccccttca gtttcgtcgt acatggtgtc gtatgcttct
     4321 gaaaagtctt taaaactcca ggcttgcgta attcgctttt gaacacgctc ttcttcggca
     4381 ggaagtattt ttgagttatt ctcaattctt aaaagaatat gagtttttgt caactcgaca
     4441 ataaaattca catataaacg acttctgtta agggtctctt caacaacggc catgtcgccg
     4501 ataacctcat tacgaaattt tttcattatt cggtcataat cggctgagtt atgtatatct
     4561 gcattgtgtt tttcaaagta gaggcgttta gcgttagcct taactgcgtt taaaagtgat
     4621 tcccttagaa tggtaaagaa agattcatgt aaaaatatta gatccgtatg tccaagaaga
     4681 ttggcaatca ttccatttaa aaaaagcata tagctttttt gaggaaatga aaaagagagt
     4741 ttaacctggt cgccagatat taccttgttt ataatctctt ttcttttatc gttcagattg
     4801 atatgctgta aagcgtataa tgccaatgga aatctcctta tgaatacatc cgaaaaccta
     4861 taaaggtata tctaatttgt caaatatgat ttttataaga tgctgtcttt cataatttta
     4921 tgtttttcaa cattattgaa taaaaaatca attatttagg agcccagctt tgcttttttt
     4981 tgatactcgt ttttttttct ttgctcgtga atgcctgtaa ccatcgggta ggactgtttt
     5041 gtaccttata ttttcttggt ttaacttttt tgtaaataat aaatagtcct ttttggaaag
     5101 aatatcaaca aaaatagctt ttttaatagg ataaagccta ataagttctg aaacccatga
     5161 ctttggagtt attgatgaaa tttgaagtgt tacagattcg atgtttcgtt tgttcagttc
     5221 gtttgctatc tgaaccgcgg atgtttcttt gtatatgacc gttgtgttac catacttatc
     5281 tggtaaaatg ctttggttgt agctccaatt attgtaaatg aaataatctg tattataaat
     5341 tatgggcacc gtgtacataa aaagcagtgc ggtaataacc agtgattttt tgatcttttt
     5401 cgatgaaggt aaaaaaaggg agcctaatag tgccatcgaa ggcaagaaaa gaacaataaa
     5461 tgattcaggt ctgcaattaa ggttagcttt actgaagatt attgtattaa gttcaagagc
     5521 tttgtaaaaa aaactgttta tcgaagacag ataaggaatc gcaaatgaca accaaaataa
     5581 aaataccttt agaaatgagc tgtatatgaa aaaaagtagt aatggtgagg cggttgcggt
     5641 tgaaatcatg gatgcgggat taaactctcc aagactaaag aatattaatg ggattactga
     5701 gagttgagcc gataaggaaa gggcaataga ttgtgataat tttaagggta tttttggaat
     5761 aatttccaaa aaatctttat aaaacagcaa tattccaaga gttgcaccga aagatagctg
     5821 aaaccccagt gaaaaaatat ctgatggatc aataatctga ataaaaacac cacttaaaaa
     5881 aagcgcgtta attgcgtttt tgcctttttt taacaaaaca aaaaaagcgt aaatccagaa
     5941 catgatagca gctcttaata atgatactgg tagctctgta ataaatgtat acagtataac
     6001 tgctgtcgat gccatgatta gagcaatact cttctttccg cttattgctc ctgctaaaaa
     6061 gaatattgat ccgcttacaa ttgctacgtg cattccagat gccgctaata gatgtaaaag
     6121 acctgctttc ttataaagat ggagcgtttt gcgggaaagg ctgctcttgt ctccaaaaag
     6181 cagagccttt ataagggcat actcctcttt tgggaacatc cttatcaatg aactatcaac
     6241 tttgcgacgt aatttttttc gaaaagaggc attttgtttt tttgttgttt tgacaaaagc
     6301 tgagtgcgtt ataccctttc ttaaaagaaa aatattaaaa tctgtatccg gtcttatctc
     6361 ttttggtttt cccgaaaccg ttatttccct gtttctgaca tctttgtcta taaaagcagc
     6421 agttttatta ccattataaa taaaacttac catgcagtta tggcgaaagt tttgctcaga
     6481 tattatttca gattcgtttt gaatcgggtg tatcataata aaaaatcgtg ttaataataa
     6541 caggctaatt attaccagag gtaatacaga gattgtaatt aatcgtttaa aagtttttaa
     6601 aacgaccgaa taggcaacta aagcggttaa aattgttaaa gctaatacaa taggaaacgg
     6661 tttaacggtt tcaggcaaat aaaaccagta taacagaaga ttaagcagtg taatataaac
     6721 aagtaaaatg aaggagggga gtctgatagc ggaatcaagt tttttaaaga tttcccacct
     6781 ttgctgctgc gccgattgta attccatagg ttaacttcct cccttcggag cctttcccga
     6841 aatatccgta accaaggtca aaatcgcata atagtgacca gtcggaagat aaaggatgga
     6901 gtaaacctaa tgtaatcaga gcaccacctc cgtcatattt ttcggaagca ggaggcattt
     6961 cgataaagaa acctgctccg cctgtaagcg tgactggaag attttcttta tacatgtact
     7021 tgatccttgc atccgcttta acatgattta tcgaagttag ttctgagttg ttaagattaa
     7081 ttgcgaatcc aagattaaac tgtcccataa gtggaggagc gattataaaa gcgcaaacag
     7141 ctggaaaaaa atagtatcct gattcttcaa tggaactctg tttgccgctt tcccaaccgc
     7201 tataagagat actgtttaat ccggcggata cggtagaacc tatgtaggtg tagtatctgg
     7261 tatttctatc ttctccggag aatgtttctg gttctttatt ttctattacg ggataatcat
     7321 tagttgaagc tgcatcctga gtttcatcgt cgttgtttgt ttccggagaa atttcagtgg
     7381 tttctgcagc aggatcgtaa ttatcaaaat ccgctgcagt tccatctgtt tgagaaaaaa
     7441 ggggaaagaa aattgccagt gtgataaaaa gaattattac agcaactgaa tacctcatta
     7501 ttgaatattt agcggattat ttacaagctg tgcgtttgcg ggaacactgt agtcaaacat
     7561 accgcctggt aatccaagat tcagttgaac cccagacaga tttacactaa aaccaccacc
     7621 tgacgaatta ttaaaagtag cacttcgcaa catatacgac gcatcggtta taatggtaat
     7681 gtcgtcatag tatccgcttc ctgtaagttt aattacagca ccacttccac cttctcgagc
     7741 catagctgtg tatccatcaa ttaatgatgc tattccaccg ctgccaccac cccctaagtc
     7801 ttgaacaccg caaataccac tttcgggatt ataaacccaa agtttgcgtc cgtttgagac
     7861 tatggttttg ccgttatcga actgaacatg aatctttccc ggagacttgt aactgaaagt
     7921 tccgttcatc atgccacctt cagtagacat ggagatcatt cctcgcatag atccaataga
     7981 tctcatacgt gccctgaatt ttgaaagagc ttcgctgccg gttattgaat aaagaggcaa
     8041 cgcgaatagt agtatcaata taaaaataat acgtttcatc tatccatcct taatattgta
     8101 gactgaattt atttgaaaat tttaaatttt ttttaatatt ctttaaaaaa aaatataatt
     8161 tagccccatt tgtgtcaaat aaaacaaata agtttcaact caatgcagat cattatgtaa
     8221 cagtaaaatt tgcaataaaa aatttgttaa aaactgattg ccgttattga gtgttgacta
     8281 tagtatacca gaacgataaa tagtgggagt ttgtgttgaa acgaaaaaaa aagaaaaaat
     8341 cgggcttaaa atcagctttt ttttacttgt tggtggttat cgctctgatt ggtggagttt
     8401 tttcagcatt ttatagtgat attgtttata agcattatct gcttttctat tatcatataa
     8461 taagaaaaga ctcctttaat atatattata aagagcttgt agaactctat aaatctgaag
     8521 atagtaacga tttcctctct tatgccactc gactttctct tatatatcct gaaagaaaag
     8581 agttaaaaaa aatcgttgcc gctcactatt ttaaaatcgg tgagaatcat gccggatttt
     8641 taccagccct gcaatcgctc acttatagtt cggctgacat tcctctgttg gagcgttttg
     8701 tgccggctct ttttgctgaa aacatgtact ctgatctatt atcaattggc tcaaagtaca
     8761 gattagatga tataaaaggg gctaacggta tagtactgta ttacggtgca gccttgtatg
     8821 aaacaggtaa ttacaaaaaa gcagtttctg ttttatcctt attgaaaggg gaaaatagtg
     8881 acccgttata ctatttgtac cttgggaaga gttgcgaaaa acaggcgcta gaacttagtg
     8941 gttcgaatag aagatcaatg tttgaaagtg ccctgtctca tcataaagag ggtctaaata
     9001 attatccagg ccataaagag ctttataacg cagtagtcag agtcttaaaa gagcttaagc
     9061 gtgataacga ggctgcggaa ttaattggtg aatacaggtt acgaaaacaa tgagaaggtt
     9121 tctcttattt acgataatgg tgcttttttt tgttaccgtc atggccgaag gtattctctt
     9181 tgatgagcct gagaaagggg ctctgcttct tcttgaaaaa tacagtaatg aaagctaccg
     9241 tatcgctgtt atgacttcca aaaacagtta ctcgttttca ggccgtaaag tttacggtga
     9301 tcctttcaaa acacatttga aaggggattc ccgttacgta tttctctcct cgctatctgt
     9361 ggctatacat gagagtaccc attattacag tgaaatatac tcattgaaac gaattgctaa
     9421 aaaatccagg ataaaatctg ctgatgatta tttttgttac tatgaatcct ccggtcaagg
     9481 tatccttgta aaagtaacaa atgtttttcc aagcagagat ataaaagata ttattgatga
     9541 gaatttgcgc ggcatctatt ataaagtata tatcgacagt aatgacagtt ctttggtttc
     9601 acagggtatg ggtatttatg gtttacttga cgagatgaat gcatatataa ggggagcacg
     9661 agttgtgctg gatctttatc ctctttatat acagaacaat cctggagagg ggctaattcg
     9721 atatcaggaa gacatagttc ctgatattac agctgcgcta gattttagat actatatttt
     9781 tgcctatctt attcatgcac agaataatcg cccgaatatc tataaaagca tagtaaataa
     9841 tagatcattt ttaaccgcta tgattaaaat tgataatgat cttcacaaac ttttaagcga
     9901 ttattatcaa aataagaaag aaattgaaaa acagtattca aaattttcaa taaaaatcag
     9961 tgaaagtggc tcattgatga ctatcgattt tccagatgag tcaagactta gaacagtatt
    10021 aaactgtgag cactatagat taatgaatgc caaatttgat gaagcgagtt atcaagcaat
    10081 tgcaaaagta ttaggtgtat tcgaaaaact gccaaggtac aaatctttta agtgaaaaca
    10141 tgttttggtt atctcaattg acttactcct gaacaagata attgaaatta attacaacaa
    10201 aataaaaagc aaacacgata cccttataaa agggtaagtt gtataggtta tgtaaaaatt
    10261 cagagtttat taaatttggg taaaagataa tgaaatcagg taaagtggtg tccctgaaaa
    10321 agggacgaga aaaaagccta ttgcgtagac atccttgggt atttagcgga gctgttaaag
    10381 agtttgataa agatatttct ccgggagaga ctgtgatagt gaccgattac tccggaaagt
    10441 ttcttgcata cggttcatat tctccggtgt cgaatattac ggtaagagtg tggagttttg
    10501 aagagagtga gcaaattgat tgctacttct ttgatagaat gataaacaag accgttagta
    10561 ggaaaaaggc tttggggttt acatgtgata ataggtcggc atacaggatt attcatggag
    10621 aatctgacgg tttacccggt attatagcag attactatgc aggttttgtt tgtgtccaat
    10681 tattaagttg tggtgctgaa ttttgaaaaa aatgatatca taaactcgct tatgaatcat
    10741 acttcgtgca aagggatcta tgaaagatct gatacctctg ctagggcaaa agaggggctt
    10801 ttaaattcaa gtggtcattt aagtggagag ataccgcccg agaatttggt tattgaggag
    10861 aatggtgttt tattacacat agatattata aacggtcaca agactggata ctatctcgat
    10921 cagcgtgaaa gtcgtcgaaa actgtctgat tacgcaagtg gagcttctgt attaaattgt
    10981 tttgcgtata ctggaggttt tggtcatttt gcctgcaaag gtgaagcttc atcagttact
    11041 catatcgaga taagtgattc agcagtaaaa cagatcaaaa aaaacgcaga gcttaacggc
    11101 ttttctgata aaaggattag ggttctaaaa ggcgatgtct ttaaagttat gcgaagctta
    11161 ttggagcagg aacaatattt tgatattatt gtccttgatc ctccaaaatt tgcagattcc
    11221 cggtttaata ttcaaaaagc ggcgcgaggc tacaaagata taaacatgct ggcctttaaa
    11281 ttgcttaatc cctttggtat tctttttacc tttacatgct ctggtgcagt tgatgaactt
    11341 ttgtttcaga aaattgtggc tggagcggca ttggatgcaa aaagagatgc ttcgataatt
    11401 gattatttag gacagtctgc ggatcatcct gtagaccttg cgtttccaga aggtagatac
    11461 ttgaaggggc ttgtatgcca gtgtcttcaa taaaacctat atataagaaa tggttaatct
    11521 gctttgtttt aagaatttaa ataataaaaa gagttttttt gtatttataa aaaaaagata
    11581 ttgccatcta tttgtcgttt tctaacatgc gggaacaata aggattttag gagataaaat
    11641 gaatagacag gaattggttg aactacttgt taaggataaa aatgccggat ttgaatccaa
    11701 ggctgctgct gacagggctc tcaaggctgt tattaatggt ataaccgagg caataaagaa
    11761 agacggttct cttcagcttg tagggtttgg aacttttagt gtga
//
LOCUS       DAOTGZ010000071        11771 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_3064
            NODE_6496_length_11771_cov_7.798481, whole genome shotgun sequence.
ACCESSION   DAOTGZ010000071 DAOTGZ010000000
VERSION     DAOTGZ010000071.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868088
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 11771)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 11771)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:22:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,785
            CDSs (total)                      :: 2,751
            Genes (coding)                    :: 2,732
            CDSs (with protein)               :: 2,732
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 12 of 19
            Pseudo Genes (internal stop)      :: 5 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11771
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6496_length_11771_cov_7.798481"
                     /isolate="AMR_MDS_3064"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            52..657
                     /locus_tag="P5123_06315"
     CDS             52..657
                     /locus_tag="P5123_06315"
                     /inference="COORDINATES: protein motif:HMM:TIGR00731.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0008097 - 5S rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA];
                     GO_process: GO:0006950 - response to stress [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L25"
                     /protein_id="HRX15908.1"
                     /translation="MGTLMLKAEKRAVTGKNNNRRLRVNGMIPAIMYSHGEAETLSVN
                     AKEFGSVFKGHISESVILTLSVDGKDHETIVKDYQRNPISGDLIHLDFYKVTAGEKIH
                     TIIPIEFTGSAVGVKKGGVFEPVEREVEVEVLPRELPEKIVVDVTNLEIGESVHISDL
                     KVPESMTFKQDADHVLAHVIIARSAAADEDEEDAEEESETV"
     gene            708..1316
                     /gene="pth"
                     /locus_tag="P5123_06320"
     CDS             708..1316
                     /gene="pth"
                     /locus_tag="P5123_06320"
                     /EC_number="3.1.1.29"
                     /inference="COORDINATES: protein motif:HMM:NF013368.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004045 - aminoacyl-tRNA hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminoacyl-tRNA hydrolase"
                     /protein_id="HRX15909.1"
                     /translation="MKLIVGFGNPGDKHVNNRQNIGFKVIEILANNANIDVRVKKKKS
                     IIGRGKIDNQDVVLLKPQTFVNLIGESVLYIASFLRINVKDVICIFEDSTLDLGDLRV
                     DYVLSDIHHEGVISMEKGLKSDRFAKVRIGVGSPGKGEKMEEYLLKDFTDEEKLILID
                     VLNKSESVIFDLLRNDIETVQNKYNPEGAPKIKRRVVKLRKV"
     gene            1742..3055
                     /locus_tag="P5123_06325"
     CDS             1742..3055
                     /locus_tag="P5123_06325"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRX15910.1"
                     /translation="MRKIQKIIIWQIVLFSLFWNGYVKAQGFEVDDERSYKDLMDAGE
                     YEKAQEKIFAKLKEINSKNTKNKRIPSNFISFVSIEQEIDINSLFNERTVENFLIEDN
                     PELHQLHLDAALVNYQLKEYKRAASYLHQSLRYKPIEFKKDDNLFSLMSQIYLNMDNI
                     TAYRNSLELAFKLNPAISKYSKDLGLSYYESSQKKKAIYYLVNYVKMESYEIEDKKIF
                     VKIANLNADINRNLEAQKYYLLYLEKSGDDYDIYYVVGNLAFNETGNLDLAQTCYMQV
                     VQNMKDEESLKKSKSMEHLGDIAFKKMYYNAALEWYLQSIELEKGYRDNLENKRIEIK
                     DYEEKINNLKIELLKSNNYVQYNEYQFLRQELARLKREEIQLNYELRKHASGKRRWKT
                     AIVYEKTEQLNKAIDYYRESIKLDYKSNEARERIEKIQLKINRGY"
     gene            3109..3861
                     /locus_tag="P5123_06330"
     CDS             3109..3861
                     /locus_tag="P5123_06330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011416652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebC/PmpR family DNA-binding transcriptional
                     regulator"
                     /protein_id="HRX15911.1"
                     /translation="MSGHNKWSTIKRKKGANDAKRGAIFTKLIREITVAVKNGGEDLN
                     SNPRLRTAVAKARENNMPMDNIERAILKASGKMEGVVYEEMRYEGYGPGGVALMVDVI
                     TDNKNRTHPEIRTIFGKNGGNLGESGCVGFMFDRKGMIIIDPDQTDEDTVMELTVDYD
                     IEDIKVNDDNSITIITSPESFNDVNDLITSKSFSTSFNEITFIPQTTVDLDEKKAEQC
                     MRLVELLEDHDDIQNVYGNYEISDEIMEKIQS"
     gene            3861..4328
                     /gene="ruvC"
                     /locus_tag="P5123_06335"
     CDS             3861..4328
                     /gene="ruvC"
                     /locus_tag="P5123_06335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015042925.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008821 - crossover junction
                     endodeoxyribonuclease activity [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="crossover junction endodeoxyribonuclease RuvC"
                     /protein_id="HRX15912.1"
                     /translation="MRILGIDPGYGILGWGVIDKGLKIVDYGAIETPSTMPLDERMFV
                     IYTELSDIISKYKPDTCAIEKLFFSKNTTTAMNVANAVGVILLVLHQYIVLYTEYTPI
                     QIKQAITGFGRADKKQMQTMVKNIFGLKEIPQPDDAADALAIAACHSFATGDK"
     gene            4467..5120
                     /locus_tag="P5123_06340"
     CDS             4467..5120
                     /locus_tag="P5123_06340"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013755.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3'-5' exonuclease"
                     /protein_id="HRX15913.1"
                     /translation="MEEFQYSISKEEINECELGSFEGDIHLISDEKQAQQIIDTLSKE
                     VSIFGFDTESKPSFRKGEFHPIALVQLATNERAYLFRVNCMKNIPDFSPLLCDESIIK
                     VGLGLKDELYEVKKTLGVTSRGFIDLEKIAAYHKFHQRGVRALAAYFLQIRISKSAQK
                     SNWERADLTDQQLRYAATDAWVCLKIYDSMIEKGFLPLENPSDMFVELKANKESEKN"
     gene            5107..5742
                     /locus_tag="P5123_06345"
     CDS             5107..5742
                     /locus_tag="P5123_06345"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025217.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HRX15914.1"
                     /translation="MKKINKYCFEHVKDYYEQNINSYSENHQILGWESREAQHIRFSV
                     LVDNVDLYKRSLLDVGCGLGDLFFYLCSQGVELEQYYGVDISEVVVNKALEMNPHLNI
                     EHRDIFSDQDFVQNNSYDVVYASGIYNLKQNNNINFLEESLSIYKKVSKEYVVFNLLS
                     EKSEDKEDAYYYYSSNCITELVRKFKFAEFSIIEDYLHNDFTVICKVNNES"
     gene            5732..6364
                     /locus_tag="P5123_06350"
     CDS             5732..6364
                     /locus_tag="P5123_06350"
                     /inference="COORDINATES: protein motif:HMM:NF012955.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HRX15915.1"
                     /translation="MKVETFPNGPLSVNSYLLYKNRVAVVIDPGHDLAGLINRCKSMN
                     LEVQAVLLTHGHLDHIAGVAQVLEKYPKCSVKIDRNDLEMAGALKMQAQMLMMDCPDG
                     FEIDDVLDTPCKLNTELGEFEVVRISGHSSGSVCFIIDNMIFSGDTLFNFAVGRTDLL
                     GSTSNKELIENINNKLIKKFGDTTRVFPGHGPETTIGFEKMNNIMLKGKI"
     gene            6489..7571
                     /gene="carA"
                     /locus_tag="P5123_06355"
     CDS             6489..7571
                     /gene="carA"
                     /locus_tag="P5123_06355"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011172311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005951 - carbamoyl-phosphate synthase
                     complex [Evidence IEA];
                     GO_function: GO:0004088 - carbamoyl-phosphate synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0009220 - pyrimidine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing carbamoyl-phosphate
                     synthase small subunit"
                     /protein_id="HRX15916.1"
                     /translation="MAKKGYLILEDGFELEGYSFGYEGENLGEIVFNTSMSGYQEILT
                     DPSYCGQIVTMTYPMIGNYGVNSEDVESSKVQVAGFIVKEYSKNYSNFRADKSLSEYL
                     IENKIPALEGIDSRLVTTHIRDKGALRGGIFFSKEGAVEKLKAFPSMSGLDLASKVSV
                     TEKYDFSKRENGPVIAAIDFGIKTNILRLLEESGFKVKVFPCNVKIEEIGDVSGYFLS
                     NGPGDPEAVECGVSLAKEIIKTGKPCFGICLGHQILSIALGGKTYKLKFGHRGGNQPV
                     KNIKTGRVEITSQNHGFAVDFDSLKDNSDIKITHINLNDNTVEGIEHCSLPLFSVQYH
                     PESNPGPNDSRYLFDEFFKRVGKMAV"
     gene            complement(7654..9840)
                     /locus_tag="P5123_06360"
     CDS             complement(7654..9840)
                     /locus_tag="P5123_06360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017812977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0005992 - trehalose biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional alpha,alpha-trehalose-phosphate
                     synthase (UDP-forming)/trehalose-phosphatase"
                     /protein_id="HRX15917.1"
                     /translation="MSKTIIVSNRLPVNITIEQDKVYYKESIGGLATGLKSFHEDGNT
                     LWVGWDGLDCEDVPEDLKMPIYNELQSQYSCIPVELSSDEINDYYLGFCNKTIWPLFH
                     YFQNTTVFDPHLWNSYKQVNNKFFEEIKKIIKKNDTIWIHDYQLFLLPELLRNHDSTL
                     TIGFFLHIPFPSYELFRQLPWREEILWGLTGSDLIGFHTFDYARHFISSIRRVLSLDA
                     KGQSFYHKDRTILVDVFPMGIDYNKFNTAINEKNIINSNNEYAMKTRGNQVVLSVDRL
                     DYTKGIPERIKAVDLFFTENSDYLEKVSFIMIVAPSRSDVDTYSLLLKEIEELVSHTN
                     GKHGTIGWTPIMFFYKPFPFEELVSLYNLADVLLVTPLRDGMNLVAKEYIASRPDKLG
                     TLILSETAGAATELGEALIVNPNDLQMISHQLKVALEMPKREQIERNSILHKRLERYN
                     IFFWASEFMRKLRSIHLRQTSIKSILLKDQEEINFIKAYKSAEKRLIFFDYDGTLVMF
                     NNSPSKAVPDLELKQILTKLTEDPKNIISIVSGRQKEFLEEHFNPFNVNLIANHGLSF
                     KEQNKEWIETEIVHNDWQKEIRPIIQMFTDNTPGSFIEEKTFSLAWHYRRCDPDLSSL
                     RLNDLRETLLDLTRTMNLTIMDGNKVLEVKDSSVNKGKGIQSMLIHHTDVPFVLAVGD
                     DHTDEDMFHILSEKDYSIKIGTGKTEARFHLPDPSSFRALLNKIIE"
     gene            complement(9837..11675)
                     /locus_tag="P5123_06365"
     CDS             complement(9837..11675)
                     /locus_tag="P5123_06365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010663811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 15 protein"
                     /protein_id="HRX15918.1"
                     /translation="MEKKNLDYGVIGNCQSAALISKEGSVEWLCLPNFSSSSIFGSLL
                     DDKNGGHFSIKMSGDYTIQQSYEYKTPILRTQFTSKDNNDSFMITDFMPRYLHPARGY
                     HQPPEIIRIVTPLKGNPVVNIDFKPALNYAKHPTTIIEEENFLKMFSTNGKYDSAYLY
                     SNLSNDINVLNGNHQLTDELFFIVSYNQKLLTPNLEKAYLEYYRTKTYWLDWVERREW
                     PLLYLDQVIRSAITIKLLTYQKTGAIIAAVTTSLPETIGEERNWDYRFCWIRDSSMII
                     SVLTRLGDNGSAERFLSYVLKLIPNKDEMIQIMYGIHGEKNLTETELPWLNGYCDSKP
                     VRIGNAAYEQKQNDIYGVLLDVILKNFYYFQMNIDQGEHLWTVVRNVARIVENHWQEP
                     DMGIWEFRSQKQNFVFSKVLCWVAMDRASKIAKLLQQPKYEKQWRDCAKTIKNDIETN
                     GWNEELQAYTQAYENSAMDAANLLMCDYEFVSPDNPRFISTVKRIKDDLCIDGLMYRY
                     KNNDDFGSPKSSFTICTFWLIKALYKIGEKKEAISLFNNILKYSNHLNLLSEDMDFKT
                     KRLLGNFPQGYSHLALIDVAWEISEDELSEEGRFVHTLETLNLEGI"
BASE COUNT         4010 a         1717 c         2287 g         3757 t
ORIGIN      
        1 ggagaatctg taagtaatct tttcgtataa taatatttta gtgaggaaaa aatgggaaca
       61 ctaatgctga aggctgaaaa aagagccgtt acaggaaaaa ataataatag aagattaaga
      121 gttaatggaa tgatacctgc cattatgtat tcgcatggtg aagctgagac tttatctgtt
      181 aatgcaaaag aatttggatc agtttttaaa ggacatattt ctgaaagtgt tatcctgact
      241 ctgtctgtag atggcaaaga tcatgaaacg attgttaaag attatcagag aaatccaatt
      301 tctggtgatc tgatacatct tgacttttat aaggttactg caggagaaaa aatacatacg
      361 atcattccta tagagtttac cggatctgct gtaggtgtca aaaaaggcgg tgtttttgaa
      421 ccagtagagc gagaagttga agttgaagtt cttccaagag aactgccaga gaaaattgtt
      481 gttgatgtaa caaatcttga gataggtgaa tcagtacata tatctgactt gaaagtacca
      541 gaatctatga ctttcaaaca ggatgcggat cacgttcttg ctcatgttat tattgcacgt
      601 tctgcggctg ctgatgaaga tgaagaagat gcagaggaag aaagcgaaac tgtctaattt
      661 tgtttttttc gtgatcctgt ttaataacaa ttacaggagt tagctttttg aaattaattg
      721 ttggatttgg aaatcctggt gataaacacg taaataatcg ccagaatatc ggttttaaag
      781 taatcgaaat tctggcgaat aatgccaata ttgatgttcg tgtaaaaaag aaaaaatcaa
      841 taatcggcag gggcaaaata gataaccagg atgttgtttt gcttaaacct caaacttttg
      901 tcaatttaat tggcgaatcg gtactttata tcgcctcctt tcttcgaatt aatgtaaaag
      961 atgtaatctg catttttgaa gattctaccc tggacttagg tgatttaaga gtagattatg
     1021 tcctttctga tatacatcat gaaggggtta tttctatgga aaaaggactt aaatcggatc
     1081 gatttgcaaa agttagaatt ggtgtaggtt ctcctggaaa aggagagaag atggaggagt
     1141 atcttcttaa agattttact gatgaggaaa aactaattct tattgatgta ttgaataaat
     1201 ctgaatcggt gatttttgat ttattgagaa atgatatcga aacagtacaa aataaatata
     1261 acccagaagg tgctcctaag ataaaaagaa gggttgtaaa attaagaaaa gtataatttt
     1321 cgttaaatga aactccacag tccttttagc ataaaaatta tttattagct tacctcgaat
     1381 taatcctctt gtgaatagta tcttgcccca atctcttctc tgttttgaaa ttattttcag
     1441 ctttaattta taaaaagaac aatcgtgtaa taaatatggt atagcatgcc atttgcaaaa
     1501 aaattaacgt ttaaaagaat aaaaacaatt aaacagtttt tttaaaaaat ttagaaagtg
     1561 aaatttatca aatattctaa aataatagtc cgataatgaa gtataatata gattaagttt
     1621 cttgatataa aaagtattcg gcaatataaa atctgtaata atttgtaaca aaaaaggata
     1681 tttggaagct ttattctgta taccgtaaaa agtatttaat aaaatacttt tggagatgga
     1741 tttgagaaaa attcaaaaaa ttattatttg gcaaattgtt ctattttcac ttttttggaa
     1801 tggatatgta aaagcacagg gttttgaagt cgatgatgag cgttcgtata aagatttgat
     1861 ggatgctggc gaatacgaaa aagcccaaga gaaaattttt gctaaattaa aagaaataaa
     1921 cagtaaaaat acaaaaaaca aacgtattcc ttccaatttt ataagtttcg tgtctataga
     1981 acaggaaatt gatataaact cactttttaa tgaaagaacc gtagaaaatt ttttaatcga
     2041 agacaacccg gaactacacc agcttcattt ggatgcggca ctggtaaatt atcagctaaa
     2101 agagtataaa agggctgctt catatttaca tcaatcgtta agatataaac caatcgaatt
     2161 taaaaaggat gataatctgt tttcattaat gtcacaaata tatctaaata tggacaacat
     2221 tactgcatac agaaacagtc ttgaacttgc cttcaaattg aaccctgcaa ttagtaaata
     2281 ctcaaaagat ttgggattat catattatga gagcagtcaa aagaaaaaag cgatatatta
     2341 tcttgttaac tatgttaaaa tggaatcgta tgaaattgaa gataaaaaga tatttgtaaa
     2401 aatagcaaat cttaatgcag atataaatcg taatttggaa gctcaaaaat actatctttt
     2461 gtatttggaa aagagtggag atgactatga tatttactat gtggtgggta atctggcatt
     2521 taatgaaact ggaaacctag atttagctca aacctgttac atgcaggttg tacaaaatat
     2581 gaaagatgaa gaatcgctaa aaaagtcaaa aagcatggaa catctaggtg atatagcatt
     2641 taaaaaaatg tactataatg cagctcttga atggtatttg caaagcatag aattagagaa
     2701 ggggtatcgt gataatcttg aaaataaaag aatcgaaatt aaggactatg aagagaaaat
     2761 taacaattta aaaattgaat tactaaaaag caataattac gtgcaataca atgagtacca
     2821 atttttgagg caggaattgg ctagattaaa gcgggaagaa attcaattaa attacgaatt
     2881 gcgtaaacat gcctctggta aaagaaggtg gaaaaccgcg attgtatatg aaaaaactga
     2941 acaattgaat aaggcaatag actattatag ggaatcaatt aaacttgact ataaatcaaa
     3001 tgaggcgcgt gaacgtattg aaaaaattca gttgaaaata aatcggggtt attaatctat
     3061 gggaaagttc tactgaataa atgaattttc aaataatgga gtaatattat gtcaggtcat
     3121 aataaatggt caacaatcaa gagaaagaag ggtgccaacg acgcaaagcg aggtgcaatt
     3181 tttacaaaac taatacgtga aataactgta gcagtaaaaa atggcggaga ggatttaaat
     3241 tcaaatccgc gtttacgaac tgctgtagca aaagctcgtg aaaataatat gccaatggat
     3301 aatatagaac gagcgatatt aaaggcttcc gggaaaatgg aaggtgtcgt ttatgaagag
     3361 atgcgatatg aaggatatgg tccaggtggc gttgcgttga tggttgatgt aataacagat
     3421 aacaaaaaca gaactcatcc tgaaataaga actatttttg gtaagaacgg tggaaatctg
     3481 ggtgaatcgg gatgtgtcgg ttttatgttt gatcgcaagg gaatgattat tatagatcca
     3541 gaccagactg atgaagatac ggtaatggag ttaacagtgg attatgatat tgaggatatt
     3601 aaggtaaatg atgataattc aataactatt attacctcgc ctgaatcgtt taatgatgta
     3661 aatgatttaa ttacttcgaa aagtttctct acatctttta atgagataac ctttatacct
     3721 cagacaaccg ttgatcttga cgaaaaaaag gcagaacagt gtatgcgcct tgtagaactg
     3781 cttgaggatc atgatgacat tcagaatgtt tatggtaatt atgaaatttc tgatgaaata
     3841 atggaaaaaa ttcaaagctg atgcgaatat tgggtattga tcccggatac ggaattttgg
     3901 gatggggagt catcgataag ggtttaaaaa ttgtagatta tggagctatt gagaccccct
     3961 cgacaatgcc cttagatgag agaatgtttg taatttatac cgagctttcc gatataatat
     4021 ccaagtataa accggatacc tgtgctatcg aaaaactgtt tttctctaaa aacactacta
     4081 ctgccatgaa tgtggcaaac gctgttggcg taatattact agtgttacac cagtatattg
     4141 ttttgtatac cgaatacaca cctattcaga taaaacaggc aattaccggt ttcggaagag
     4201 ccgataaaaa acagatgcaa acaatggtta aaaacatctt tgggttgaaa gaaataccac
     4261 aacctgatga cgcagcagat gctttagcga tagctgcttg tcattcgttt gcaactggtg
     4321 ataaataata agatttttga aaactatttt aaataaataa aaaataaggt tttaggaact
     4381 gtactttttt ttggtttata tgagataatt aggcaatttt cttgacgtac aagagagcca
     4441 ttaaacaata aagtcaggaa aaaataatgg aagaatttca atattcaata agtaaagaag
     4501 agataaatga gtgtgagctt ggaagttttg aaggtgatat acatttaatc tctgacgaaa
     4561 aacaggcaca gcaaataatt gatactttgt caaaagaggt ctctattttt ggttttgaca
     4621 cagagtccaa accatcgttc agaaagggcg agttccatcc tattgctctg gttcaattgg
     4681 ctaccaatga aagggcttat cttttcagag taaattgcat gaaaaacata cctgattttt
     4741 caccactgtt atgtgatgag tcaattataa aggttgggct agggttaaaa gatgaattat
     4801 atgaggtcaa aaagactctg ggagtcactt ctcgaggatt tattgatcta gaaaaaattg
     4861 ccgcatacca taaatttcat caaagaggcg ttagagcctt agcagcgtat tttttacaaa
     4921 taagaatttc aaaatctgct caaaaatcta attgggaaag agctgattta actgatcaac
     4981 aattgagata tgcggcaact gatgcatggg tttgtttgaa aatttatgat agcatgattg
     5041 aaaaagggtt tttaccatta gagaatcctt ctgatatgtt tgttgaactt aaggcaaata
     5101 aagaaagtga aaaaaattaa caaatactgt tttgaacatg taaaagatta ttatgaacaa
     5161 aatattaaca gttattccga gaaccatcaa attctaggat gggaaagccg ggaggcacag
     5221 catatccggt tttccgtatt ggtagataac gttgacttat ataaaagatc tttgttggac
     5281 gttggatgcg gcctaggaga tctttttttt tacctatgta gccagggtgt tgaactggaa
     5341 caatattatg gtgttgatat ttcagaagtt gtagttaata aagctttgga aatgaatccc
     5401 catctgaata ttgaacatcg tgatatattt tctgatcaag attttgtgca aaataatagc
     5461 tatgatgtag tatacgcatc gggcatatat aacttgaaac agaataataa tataaatttc
     5521 ttggaagagt cattatcaat ttataaaaaa gtatcgaaag aatatgttgt tttcaatcta
     5581 ttaagcgaaa aatcagaaga taaagaagat gcttattatt attactcttc aaattgtatt
     5641 actgagttgg taagaaaatt taaatttgca gaattctcga taattgagga ctatttgcac
     5701 aatgatttta cggtaatttg taaggtgaat aatgaaagtt gaaacatttc caaatggacc
     5761 actctctgtt aatagttatt tattgtataa aaatcgagtt gctgttgtta tagatcccgg
     5821 gcatgattta gccggattaa ttaatagatg caagtcaatg aaccttgagg ttcaagcggt
     5881 tctacttacg catgggcatc ttgatcatat tgccggtgta gctcaagttt tagaaaaata
     5941 cccgaaatgt tcggtaaaaa ttgatagaaa tgatttggag atggcaggcg cgttaaaaat
     6001 gcaagctcaa atgcttatga tggattgtcc tgatggtttt gaaatagacg atgttttgga
     6061 tactccttgt aagctgaata cagaattggg agaatttgag gttgttcgaa taagtggaca
     6121 ttcttcagga tcggtatgct ttattattga taacatgatt ttctccggtg ataccctgtt
     6181 taatttcgct gtagggcgta cagacctgtt gggaagcaca agtaacaagg aactaatcga
     6241 aaatataaat aataaattga taaaaaaatt tggtgacact acaagagttt tcccaggtca
     6301 tggaccagaa acaacaatag gtttcgaaaa aatgaacaat attatgttaa agggaaaaat
     6361 ctaggatatt atagaataat tacagcaaga tagttatttt tattaaatga aataaatctc
     6421 aggttaggtt ttataaaatt gataatgtac aggtgttaaa ttttaagatc tgttaagaag
     6481 aggataaaat ggcaaagaaa ggttatttga tacttgaaga cggatttgaa ctggaaggct
     6541 atagttttgg atatgaagga gaaaatctcg gagagattgt ttttaataca tctatgtcgg
     6601 gatatcagga aatattgacg gacccttcct attgcggaca gattgttact atgacttacc
     6661 caatgattgg aaattacggt gttaacagtg aagatgtgga gtcatcaaaa gttcaggtag
     6721 cgggtttcat agtaaaagag tatagcaaaa attattcaaa tttcagggct gataaatcat
     6781 tatctgaata tctgatagaa aacaagatcc ccgctctcga aggtatagat tccaggcttg
     6841 ttacaacgca tattcgtgat aagggagctt tgaggggtgg aatcttcttt tcaaaagaag
     6901 gtgctgttga gaagcttaag gcttttccga gcatgtcagg actggatctt gcatccaagg
     6961 tgagcgttac cgagaagtat gattttagta aacgagaaaa tggaccagtt attgcagcaa
     7021 ttgattttgg aataaaaacc aacatcttac gtttacttga agaatcaggt tttaaagtca
     7081 aagttttccc ttgtaatgta aaaatcgaag agattggtga tgtaagcggt tactttttat
     7141 ctaatggacc tggagatcct gaagctgtag agtgtggggt atctctggca aaagagatta
     7201 ttaaaactgg aaagccctgt ttcggaatat gtctaggaca tcaaattttg agcattgctt
     7261 tgggtgggaa aacatataag ttgaagtttg gtcatcgggg aggcaatcag cctgtaaaaa
     7321 atattaaaac aggccgtgtc gaaataacat cacaaaatca tggatttgcc gttgattttg
     7381 attccttgaa agataactct gatataaaaa taacacatat taacctgaac gataatactg
     7441 ttgaaggaat agagcattgt tcattgccac tattttctgt tcaatatcat cctgaatcaa
     7501 atccgggtcc taatgactca agatacttgt ttgatgagtt ttttaaaagg gttggtaaaa
     7561 tggcagtgta atttgggaca attttattgt ttaatggata atgttactat ttcaaatggg
     7621 aatggctttt gaaatagtaa catttattaa tacttattct atgattttgt ttaacagtgc
     7681 tctaaaagat gatgggtcgg gtaagtgaaa acgagcttct gtttttccgg tacctatttt
     7741 aatggaataa tccttctcag ataatatatg aaacatatcc tcgtcagtat ggtcatctcc
     7801 aacggctaaa acaaaaggaa catctgtatg atgaataagc attgattgaa tgccttttcc
     7861 tttgtttaca gaagaatctt tgacttcaag aactttgttt ccatccataa tagtcagatt
     7921 catcgttcgt gttaaatcca aaagagtttc acgtaaatcg ttcagtctta acgaagagag
     7981 atcgggatca caacggcgat aatgccaggc taatgaaaaa gtcttctctt caataaatga
     8041 ccctggagta ttgtcggtaa acatttgaat aataggtcgt atctcttttt gccaatcgtt
     8101 atgtactatt tctgtttcga tccattcttt attctgttct ttaaaagata gtccgtgatt
     8161 agctatcagg ttaacattaa aagggttgaa atgttcttca agaaattcct tttgtcttcc
     8221 acttacaatt gaaattatgt ttttcgggtc ttctgtaagc ttggtcagta tttgtttaag
     8281 ttcaagatcc ggtacagcct ttgacgggga attattgaac attactagag ttccgtcata
     8341 atcgaagaag attagtcttt tctcggccga cttataggct tttataaagt tgatttcttc
     8401 ttggtctttt aatagtattg atttaatgga agtttgcctt aagtgaatag agcgaagttt
     8461 gcgcataaat tcacttgccc agaaaaaaat attgtatcgc tcgagtcttt tgtgaagaat
     8521 agaattacgt tcgatctgtt cccttttcgg catctcgaga gctactttaa gttgatgcga
     8581 tatcatctga agatcatttg gattaacaat aagggcttcg ccaagttctg tagccgcacc
     8641 agctgtttca cttaatataa gagtaccaag tttatctggt cgtgaagcaa tatactcttt
     8701 ggcgacgaga ttcataccat ctcgtaaagg ggtaacgagt aaaacatccg caagattata
     8761 gagagaaacc agttcttcaa atggaaaggg tttgtaaaag aacatgattg gtgtccagcc
     8821 tattgttcca tgttttccgt ttgtgtggct tactagttct tctatttctt taagtaacag
     8881 gctataggta tcaacatctg accgcgaggg agcaacgatc atgataaagc tgactttttc
     8941 aagataatca gaattttctg taaagaaaag atcaactgct tttatccttt caggaatacc
     9001 tttagtgtaa tcaagacgat ctacagaaag aacaacttga ttaccacgcg ttttcatcgc
     9061 gtactcatta tttgagttta ttatgttttt ttcatttatt gcggtattaa atttattata
     9121 atcgatgccc ataggaaaaa cgtctacaag tatggtgcgg tctttatgat aaaaactttg
     9181 acctttggca tcaagagata aaactctgcg aatactactt ataaaatggc gagcataatc
     9241 aaaagtatga aaaccaataa gatcagatcc ggttaatccc cataatatct cctctctcca
     9301 aggcaattgt cggaataatt cataagatgg aaatggtata tgcaaaaaaa atccaattgt
     9361 taaagtgctg tcatgattac gtaaaagttc tggtaataaa aacagctgat aatcgtgaat
     9421 ccagatagtg tcattttttt taattatttt cttaatttct tcaaagaatt tattatttac
     9481 ctgtttatat gagttccaga gatgaggatc aaaaactgtg gtgttttgaa aatagtgaaa
     9541 aaggggccaa atggttttgt tacaaaaacc aagatagtag tcattaattt catccgaact
     9601 aagttctacg ggaatacagc tatactgact ctgtaattca ttataaatag gcatctttag
     9661 atcttccggt acatcttcac agtccaaacc gtcccagccg acccataagg tgttgccgtc
     9721 ttcgtgaaaa ctttttagac ctgtagctag gcctcctatg ctttctttgt aatatacttt
     9781 gtcttgttca atagtgatgt taacaggaag tctattggaa acaataattg ttttactcat
     9841 ataccctcca gatttagtgt ttcaagtgtg tgaacaaaac gtccttcttc ggaaagttca
     9901 tcttcagata tttcccaggc gacatctatt aatgcaagat gagaataacc ctgaggaaaa
     9961 tttcctaaaa gtctttttgt tttaaaatcc atatcttcgc taagaagatt caaatggttg
    10021 ctatatttaa gtatattgtt aaaaagagaa attgcctcct ttttttcacc tattttatat
    10081 aaggctttga taagccagaa ggtgcagatt gtaaaagaag atttaggaga accaaaatca
    10141 tcattatttt tatagcgata cattaggcca tctatacaca aatcatcttt tattcgtttg
    10201 acagttgaaa tgaatcttgg gttatccggg ctaacaaatt catagtcaca cataagcaag
    10261 tttgcggcat ccatggcgct gttttcgtat gcttgagtgt aagcctggag ttcttcattc
    10321 cagccatttg tttcgatatc attttttatt gtttttgcgc aatctctcca ctgtttttca
    10381 tattttggtt gttgaagcag tttcgcaatt tttgaagctc gatccattgc cacccaacaa
    10441 agtactttag aaaaaacaaa attctgtttt tgcgaacgaa attcccaaat acccatatct
    10501 ggttcttgcc agtgattttc aacaatacgt gcgacatttc tcacaacggt ccagagatgc
    10561 tctccttgat caatgttcat ttgaaaatag taaaagtttt taagtattac atctaaaagt
    10621 acaccataaa tatcattctg tttttgctcg taagcagcat tgccaattcg aactggcttt
    10681 gaatcgcaat aaccgttaag ccagggaagt tctgtctcgg tcagattctt ttctccatga
    10741 ataccgtaca taatttgaat catctcgtct ttgtttggaa taagcttaag tacataactt
    10801 aaaaatcttt cggcactgcc attgtcaccg agacgtgtta atacactgat tatcatggac
    10861 gagtcacgta tccagcaaaa gcggtaatcc caattgcgct cttcaccaat tgtttctggc
    10921 aacgatgttg ttacagcagc aatgatggcg ccagtttttt gataagttaa aagtttgatg
    10981 gtgatagcgc tgcggattac ttgatcaagg tatagcagag gccattcacg cctttcgacc
    11041 cagtcaagcc aatatgtctt tgtgcgatag tattccagat atgctttttc gagattagga
    11101 gttaacagct tttggttata tgatacaata aaaaaaagtt cgtcggttaa ttgatgattg
    11161 ccattaagaa cattaatatc gttagataaa ttagaataaa gataagctga gtcatacttg
    11221 ccattggtag aaaacatttt taggaaattt tcttcttcaa ttattgttgt tgggtgtttc
    11281 gcgtagttta atgctggttt aaaatcaata ttaacgacag gattaccttt aagtggtgtt
    11341 actattcgga ttatttcagg aggttgatga taacctctgg cgggatgaag atatcgaggc
    11401 ataaaatctg taatcatgaa tgaatcatta ttgtctttgg aagtaaattg tgttctaaga
    11461 ataggtgttt tatactcgta tgattgctgg atagtataat cgccactcat ctttattgaa
    11521 aagtgtccac cgtttttgtc atcaagtagt gagccaaata tagacgagga cgaaaaattg
    11581 ggcaaacaca gccactcaac agaaccttct tttgaaatca gggcggcact ctggcagttc
    11641 cctataacac cgtaatcaag attttttttt tccatataac ctcagtaatc aaaattctcc
    11701 gaaccttatt taagggcgac aatatataat tcttgcccga ttgtatcttt gtcaaacagt
    11761 ttatatgcgt a
//
LOCUS       DAOTGZ010000072        11744 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_3064
            NODE_6510_length_11744_cov_4.702370, whole genome shotgun sequence.
ACCESSION   DAOTGZ010000072 DAOTGZ010000000
VERSION     DAOTGZ010000072.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868088
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 11744)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 11744)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:22:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,785
            CDSs (total)                      :: 2,751
            Genes (coding)                    :: 2,732
            CDSs (with protein)               :: 2,732
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 12 of 19
            Pseudo Genes (internal stop)      :: 5 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11744
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6510_length_11744_cov_4.702370"
                     /isolate="AMR_MDS_3064"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..126
                     /locus_tag="P5123_06370"
     CDS             <1..126
                     /locus_tag="P5123_06370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013970186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HRX15919.1"
                     /translation="VLNNGMSILGVSVDWQQVIKGLVLLGAVCFDVYTKNKSGSK"
     gene            419..2122
                     /locus_tag="P5123_06375"
     CDS             419..2122
                     /locus_tag="P5123_06375"
                     /inference="COORDINATES: protein motif:HMM:NF012245.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0098561 - methyl accepting chemotaxis
                     protein complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein"
                     /protein_id="HRX15920.1"
                     /translation="MNFKNLNISTKLYLLFFIFLFIIIFAYSSIIILFVKQNNDYRSY
                     EYNNNRIKDIQKAIYIHNSDWKSILINAQDPSGFDRAKRVFFNKTITILSDINDFKLL
                     VADDPNTLQTLDELYNSYVEMNTQYENALQLYNEDNYAESITLINKKVNRIEDVPLNN
                     LTKTVFQISDTTEKSKEALMILITLLTIISAFVFIIITFIFSRQIIISINKPLKRIIS
                     RLHDISDGDGDLTKRIDIISKDETGVLAALFDSFINNIQEIIKTIIHVISNLFKASEQ
                     LSASSSSLLIVSHKQSEIAKEMNDTISRVKLRAKTIFENSVHQNDKIDSLNQKIEILS
                     QTIIDMESKIHLATKEALVINDNVVIGDTAMHKMNKSMLTIMHSSKKIVGNIKMIDDI
                     AEQVSLLALNASIEAARAGDSGLGFAVVAEEVSKLSDLTSAAVKEIDSLVQLNHRETQ
                     VGVDTTTETMKIFNGISAGINSISGLINEINDLSERENGIKSVLTENGNAVLKITEEN
                     KNLLEEQNVSFSEISVIIDLLNEFSTKNAESAKLLSGNSSEISLIIKELDDKINNFKV
                     S"
     gene            complement(2045..2563)
                     /locus_tag="P5123_06380"
     CDS             complement(2045..2563)
                     /locus_tag="P5123_06380"
                     /inference="COORDINATES: protein motif:HMM:NF016300.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIMPL domain-containing protein"
                     /protein_id="HRX15921.1"
                     /translation="MKYSVLVFSLLILFTGCVKVYNQNPNEGGRLAISSSAELMVVPD
                     EMSILFGIEKYDSNVKNARTQTRIILDSLVKKLKEEGISEKNIEYGQISIIPRYPENQ
                     YGKRIIGYTTSLDVTVKLQNFDLISAVVDKAVSVGVNTIGNFNFYSRDFKIIYLIIKF
                     FNNQRNFRRITR"
     gene            complement(3101..4015)
                     /locus_tag="P5123_06385"
     CDS             complement(3101..4015)
                     /locus_tag="P5123_06385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009994642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRX15922.1"
                     /translation="MSKIFGDSEKYILKNLNIGNIFIYGNEKYIIRKSGKPTCSSGEP
                     KTDIYIQASNLSNSKIDIEIKISYKQNNADFLENKMTAKRAKQILGPDWKKIIIKSTK
                     KLQKEFKKRPLIYKDKHKKTARGCFTLGWKFELLLVKSGNLSYELELSQEQVHDIYSG
                     KNLSADKKDSLVNGEQILNSGIANFILKGDVSSYKNTQDIIDNIIIIDKYIQENPNIY
                     YACKALNYRSFKDKWDGPRPLAVYVNWTRKRNKLHSEIIFKSPLSVNGNLVANQLKNV
                     LMEMNIKNTNDITNNNCYFPEIIYNKIE"
     gene            4146..5201
                     /locus_tag="P5123_06390"
     CDS             4146..5201
                     /locus_tag="P5123_06390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009626781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA cytosine methyltransferase"
                     /protein_id="HRX15923.1"
                     /translation="MNIISLFAGCGGLDLGFTKAGFKTIWANEFDKEIWETFERNHPE
                     TVLDRRSIKDIDLSDIPNCFGIIGGPPCQSWSEAGAQRGISDVRGQLFFEYIRVLKNK
                     QPLFFLAENVSGILANKHSEALKNIIDQFKEAGYNVEIKLLNAKDYNVPQDRKRIFFI
                     GYRKDTNLKFHFPDPNNNIPKLKNAIWDLKDNVLPAKKFNYTNGDDCKFINHEYMIGG
                     FSTIYMSRNRVRSWEEPSFTIQAGGRHAPIHPQAPKMTFLEQNKREFVRGKEHLYRRL
                     SVRECARIQTFPDDFIFHYDNLTSAYKMIGNAVPVNLSFELAKIIHSDIIKYLKENNL
                     NICEQNMDLKQQKLALA"
     gene            5963..7243
                     /locus_tag="P5123_06395"
     CDS             5963..7243
                     /locus_tag="P5123_06395"
                     /EC_number="1.1.1.3"
                     /inference="COORDINATES: protein motif:HMM:NF004976.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004412 - homoserine dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="homoserine dehydrogenase"
                     /protein_id="HRX15924.1"
                     /translation="MKKIVNIGLVGFGTVGSGVYNVIKNNGKLIEQRTGVSLVLKTVC
                     DLRMDEVKNTVSSDVQVTDNWKSLIEDKEIDTIVELIGGLEPAKSVVEAALSAGKNVV
                     TANKKLLAEKGSVIFELADRKDAKLGFEAAVGGGIPCIEDLKNGLVANNVNHIFGILN
                     GTTNYILTRMELAGLSFADALAEAQAEGFAEADPTFDIEGYDAGHKICLLAMLAFNKK
                     IEYGSIQIEGITKIKTLDTEFARRMGYAVKLLGIAREIDGEVDVRVHPVMIRESHLIA
                     SVSHEYNAVVFDADMTGEVLLYGKGAGSLPTASAVVSDIVQIAVKKGVYESAILTKGD
                     AVILGAEKRFSRFYLRINTEDRPGILEKVAHAFSANNISVSSVEQHETGHNIVPLLFV
                     THKACEADIRKAVNEINEFDFVTEPVMMIRIEES"
     gene            7323..8660
                     /gene="thrC"
                     /locus_tag="P5123_06400"
     CDS             7323..8660
                     /gene="thrC"
                     /locus_tag="P5123_06400"
                     /EC_number="4.2.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007220637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004795 - threonine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009088 - threonine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="threonine synthase"
                     /protein_id="HRX15925.1"
                     /translation="MNVNSYFKCIKGCEGQYSLTDIIYRCPKCGSLLQVTHDTDALKA
                     KLNGEGWNKLFASRMGSTKWPYGSGVWRYKELVAPEIDDDSIVSMYEGNSNLFYAKRF
                     GQMFGVKDLWIKMCGNTHTGSFKDLGMTVLVSMVNQMIKKGKNIKAVACASTGDTSAA
                     LAAYAAYAGIPSIVFLPANKITTAQLVQPMSNGSIVLALDTDFDGCMKIVQQVTENNE
                     IYLANSMNSLRVEGQKTIAPEIVQQFDMEVPDTIIIPGGNLGNVSALGKGFKEMKELG
                     MISKMPRIVVAQAQNANPLYTSYKNGFESFSSISAKKTLASAIQIGDPVSVDKAIATL
                     KEFNGIVEQASEEELAEAAALADRTGMYSCPHTGVALSVFTKLASRGDIASDERVVII
                     STAHGLKFSEFKVGYHEETISGINCSHANKPIELAADSDLVLKVLEEEIKKLK"
     gene            8750..10006
                     /gene="lysA"
                     /locus_tag="P5123_06405"
     CDS             8750..10006
                     /gene="lysA"
                     /locus_tag="P5123_06405"
                     /EC_number="4.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020815676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008836 - diaminopimelate decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopimelate decarboxylase"
                     /protein_id="HRX15926.1"
                     /translation="MTKVHTPVTDKMDFFKESTPVQLAEKYGTPLYVYNENILRERCR
                     EMKGLVDYKNFSVNYSAKANSNLELLKIVNEEGLSVDAMSPGEIHAELAAGFTADRIL
                     YISNNVSADEMRFSVDRGIMVSIDSLSQLDLYGRNFPGTKVALRFNPGVGAGHHEKVV
                     TAGKKTKFGIAPEMMSQVKEIVKKHDLTVAGVNQHIGSLFMESDAYVKSTDSILSIAS
                     NFDSLEFVDFGGGFGIPYRKSEGQKRLDLAETGRLLKQNIDKFIKAYGREIKIKIEPG
                     RYIAAECGIILGRVHSVKENYGSRYIGTDIGFNVLKRPVMYDSHHEIEIFREKGSIDS
                     SKDCVKIVGNICETGDILANDRELPKIVEDDIIAVLDAGAYGFCMSSNYNNRLKPAEV
                     LISSDGSDKLIRRRDTLEDLMRNFEL"
     gene            10094..10981
                     /locus_tag="P5123_06410"
     CDS             10094..10981
                     /locus_tag="P5123_06410"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009975 - cyclase activity [Evidence IEA];
                     GO_process: GO:0009966 - regulation of signal transduction
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HRX15927.1"
                     /translation="MNKKIIVVDDDPVNRTLICSVLKKYTLFTAEDIKSMYNIIKENG
                     LPDLILLDVMLPEVNGFEAARDLHTEFRNQDVGIIFVSARTEGSSVKEGFDSGGYDYI
                     KKPFDSIELVARVENQLERIERENKLRSDASLDPLTAVYNRRYFKHLVEQKINFCNRK
                     NMKLSIAMIDLDNFKEINDNFSHLAGDYVLQTFASQISESLRKYDLMGRFGGEEFIIA
                     FVDCCKDCSYSIMERLRADVEKRAFIFEGNDISLSFSAGIADLCEIESRKSVYNEILK
                     LSDQRLYEAKRLGRNQIVI"
     gene            complement(11011..>11744)
                     /locus_tag="P5123_06415"
     CDS             complement(11011..>11744)
                     /locus_tag="P5123_06415"
                     /inference="COORDINATES: protein motif:HMM:NF018028.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="RDD family protein"
                     /protein_id="HRX15928.1"
                     /translation="TTVHNVNITYKAAPLGSRILAWGIDSALLLFTGIFIMVLLLITS
                     ELNFDKINFNQPSTLLPLFFIALPLMLYNLLCEIGMKGQTIGKNIMKIRVISLDGSPL
                     SIGQCLIRWTMRIIDLHISSGLPAILTIAFKESGQRIGDIAAKTAVIEDLPAKAPVTA
                     GSTFKPSVLETPCLTSTESSTIQKIITFVSNGGNKTVAEDYAKQIRDNIVDRYSLQER
                     SAEFGDKSIDFIETVLRDHTGLSAD"
BASE COUNT         3775 a         1842 c         2349 g         3778 t
ORIGIN      
        1 gttttaaata atggtatgtc tattttgggt gtaagcgttg actggcagca ggtaatcaag
       61 ggtcttgttc ttcttggtgc tgtctgtttt gatgtttata caaaaaataa atctggtagc
      121 aagtagcaat tagcaatttc tttaataaaa aactctctca taacctccgg gagagttttt
      181 tattatataa aatttttaca tgtttagtat ttgggataag tacatgaata aatttgcata
      241 aggatgatta aacatatact tatgatatat ctggaatttt aaaaaatatt taatgcttta
      301 aatctgtaaa aaccaaactt aacaacaaat tgtctatttc ttttaatctt aagtcgtata
      361 gatcaccaaa tctataaata gttgatatgt taaaattata tgatctactg gatttattat
      421 gaactttaaa aatttgaata ttagtacgaa actttacttg ttgtttttca tatttttatt
      481 tattataata tttgcttata gttctataat aatcctcttt gttaagcaaa ataatgatta
      541 ccgatcatat gagtataaca ataaccgtat caaagatatt caaaaagcta tatatataca
      601 caatagtgac tggaagagta ttttaatcaa tgcacaagat cccagcggtt ttgatcgagc
      661 gaaacgagtt ttctttaaca aaacaataac aatattgagt gatataaatg atttcaaatt
      721 attggttgcg gacgatccaa atactctgca gacgttggat gagttatata acagttatgt
      781 tgagatgaat acacagtatg aaaatgcttt acaattgtat aatgaggata attacgcaga
      841 atcaataacg ttaattaata aaaaagtaaa tcgaatagaa gatgtcccct taaataatct
      901 tacaaaaact gtttttcaaa tttcagatac aacagaaaag agtaaagagg ccctaatgat
      961 cttaataact ttactgacaa tcataagtgc cttcgttttt ataataataa catttatctt
     1021 ctctagacaa ataattataa gtataaataa accgttaaaa cgtataatta gcaggttaca
     1081 tgatatttcc gatggcgatg gtgatttaac aaaaagaatt gatataattt ctaaagatga
     1141 aacaggggta ctagcggcat tatttgattc atttataaac aatatacagg aaattattaa
     1201 aacgattata catgttataa gtaacctttt taaagcgtca gagcaattgt ctgcaagttc
     1261 aagctctttg ttgattgtct cgcataaaca atcagagatt gcaaaagaga tgaatgatac
     1321 aattagtcgt gtaaagctgc gtgctaagac catatttgaa aattcagttc atcaaaacga
     1381 taaaattgat tctttaaatc aaaaaataga aatactatca caaacaatta tcgatatgga
     1441 gtctaaaatt cacttagcca ctaaagaggc tttagtaatt aatgataatg tggtaatcgg
     1501 tgatactgct atgcacaaaa tgaataagag tatgctaact atcatgcaca gctcaaaaaa
     1561 aattgtcgga aatataaaaa tgatcgatga tatcgcagag caggtaagtt tgttggcgtt
     1621 aaatgcatcg attgaagctg ccagagcagg tgatagcggc cttggttttg ctgtggttgc
     1681 cgaagaagtt tctaagttat ctgatttaac atcagctgcg gttaaggaga ttgactcttt
     1741 ggtacagtta aatcaccgag aaactcaggt aggagtagat accactactg aaaccatgaa
     1801 aatctttaac ggtatatcgg caggtattaa ttctatttcg ggtctgataa atgagattaa
     1861 tgatttatca gaaagagaga atggtataaa atctgtatta acagaaaacg gaaacgcagt
     1921 attaaaaatt acagaagaga ataaaaatct tttagaagag cagaacgtct cgtttagtga
     1981 gatctccgtt attattgatt tattgaacga attttccaca aaaaatgccg agtcagctaa
     2041 gctgttatcg ggtaattctt ctgaaatttc tctgattatt aaagaacttg atgataagat
     2101 aaataatttt aaagtctctt gagtagaaat taaaatttcc gattgtgtta actccaacgc
     2161 ttaccgcctt atcgacaacc gcggaaataa gatcgaagtt ttgcagtttt actgttacat
     2221 caagtgatgt cgtataaccg ataatccgtt ttccatactg attctctgga taacggggaa
     2281 tgatactgat ctgtccatat tcgatgttct tttcggatat tccctcttct ttcagttttt
     2341 tgacaagaga atccagaatg attcgggtct gtgtacgggc atttttaaca ttagaatcgt
     2401 atttttcgat accaaataag atactcatct cgtcgggtac aaccataagc tctgcagatg
     2461 atgagatagc caaccgacca ccctcgtttg gattctggtt atatacttta acacaaccgg
     2521 tgaaaagtat taaaagtgaa aaaactaaaa cagaatattt catttcggtg atcctttggt
     2581 aaatttttaa aagggagtta ataaattttg caactttgta tgtattgcca ggtaaatact
     2641 ggtttgtcaa cccgttattg ctatcaataa tatggtttct ctatgacctt gtgagttgtt
     2701 gatgaatggt gtttgtgctt gtgaaaagaa ttttattgat gagacctaaa cgggcgcgac
     2761 tgttctatca gatgaacgtg ggctatacaa gcgggactat tttatcaggt gaatgagggc
     2821 taaacaggcg tgattatttt tttggatgaa cgagttcgat aaatcggaaa tgcaggcgcg
     2881 gttggttatg tgagattatt ttagtatttc ttctgttctg tactttttca atttgaacga
     2941 atttttttat aacatagaga ctgagtcgct atgttggttt atctatactt tgtatattgt
     3001 tctgttagag ctcattctgt tttggtctat ggaattttgg tatggcagga ttttttttga
     3061 ttgtaatata tttggttgtg ggggaatata agtatctcgg ttactcgatt ttattataaa
     3121 ttatttcagg aaagtaacaa ttattattag taatatcatt tgtatttttt atattcattt
     3181 ccattaacac attttttaat tgattagcga ctaaattccc atttacagat aaaggtgatt
     3241 taaatattat ttcagaatgt agtttatttc tttttcgagt ccaattgacg tatacggcta
     3301 atggtctagg accatcccat ttatccttaa aacttctata attcaacgct ttgcaagcat
     3361 agtatatatt cggattttct tgtatgtatt tatcaataat aataatatta tcaataatat
     3421 cttgtgtgtt cttataacta gaaacatcac ccttcaatat aaaattagca attccggaat
     3481 tgaggatttg ttctccatta actaatgaat cttttttatc ggcagataag tttttgcctg
     3541 aatatatatc atgaacttgc tcttgtgata attctaactc ataagataga ttaccgcttt
     3601 tgaccaataa taattcaaat ttccaaccca acgtgaaaca acctcttgca gtttttttgt
     3661 gtttatcttt gtatataaga ggtcttttct taaactcttt ttgtaacttt tttgttgatt
     3721 taataattat ttttttccaa tcaggaccta aaatttgttt tgctctttta gctgtcattt
     3781 tgttttctag aaaatctgca ttattttgct tatacgaaat ttttatttca atatctattt
     3841 tggaatttga taaattacta gcttggatat aaatatcagt ctttggttca cctgaactac
     3901 atgtaggttt gcctgatttt ctaattatat atttttcatt gccataaata aatatattcc
     3961 caatatttag gttttttaat atgtattttt cagaatcacc aaagattttt gacatttttt
     4021 ctccaaacca aaaaaaaatt ttaaacttat acgttaataa tcgtattaat agttaaggaa
     4081 ctaaagtaat tgacagatga gacataaaat attttactaa acataagata aggatggagg
     4141 agtgcatgaa tattatatct ttatttgctg gctgtggagg tttggattta ggttttacca
     4201 aggctggctt taaaacgata tgggctaatg aatttgacaa agagatttgg gaaacttttg
     4261 aaagaaatca tcctgaaact gttttagaca ggagaagtat taaagacatc gatttatcag
     4321 atatacccaa ctgctttggc attattggcg gtcctccatg tcaaagctgg agtgaagctg
     4381 gagcacaaag aggtatttcc gatgtaagag ggcaattgtt ttttgaatat attagagtac
     4441 ttaaaaataa gcaacctttg ttctttctag cagaaaatgt atctgggata ttggcaaaca
     4501 agcactctga agctttgaaa aatatcattg atcaatttaa ggaggcaggt tataatgttg
     4561 aaataaaatt gctaaatgca aaagattata atgtccctca ggatcggaaa agaatctttt
     4621 ttattgggta ccgtaaggat acaaatctaa aatttcattt tcctgatcca aataataata
     4681 tacctaaatt gaaaaacgct atttgggact taaaagataa cgtcttaccc gcaaaaaaat
     4741 ttaattatac gaatggtgat gattgtaagt tcataaacca tgaatatatg ataggtgggt
     4801 tttcaacaat ttatatgtca agaaatcgag tacgctcttg ggaagaacca tctttcacta
     4861 tccaggctgg tggtaggcat gcccctattc accctcaggc tcctaaaatg acatttcttg
     4921 agcaaaacaa aagagaattt gttaggggca aggaacattt gtataggcga ttaagtgtaa
     4981 gagaatgtgc tcgtattcaa acttttccag atgactttat ttttcattac gataacttaa
     5041 cgtctgcgta taaaatgatt gggaatgcag tccctgtgaa tttaagtttt gaattagcaa
     5101 aaataattca tagtgatatt attaaatatt taaaagaaaa caatctaaat atatgtgaac
     5161 aaaatatgga tttgaaacaa cagaaactag cacttgcata atgagaaagg ctgatggttt
     5221 tacaataaag tcatttttta gaataaagct atagaatcga agatcaaggt ataaagaaaa
     5281 atttacataa acgacctgaa aatggggtga tttgtgcaaa taaactgcaa tatttattga
     5341 tggtcaacaa acactatatt caaataccag tccctgttaa taaatcaggt aacaacatga
     5401 actaaaaaaa tataagatag catttatccc ggtttttata agaattattc tggcaaatcc
     5461 tcttgctttt attctgaaat acaaaagagc cttttgcaag cacctttgcc cagcttctcg
     5521 gatcatgaaa ccggcaagtt atttttagtc ttgttaaact aaaaccaaat atgagtgtca
     5581 aatgtaaaga gtgctcactt tgcaataatt actgcccaat gggtgttgat gttttgggtt
     5641 atatgcgcga gaataaaccg gtaactgata ccgagtgtat attatgcggt gattgctcaa
     5701 acgtatgtcc ggttggaaaa atattgtaag aaagttaaac aatgatattt gtctcaataa
     5761 acaaaacatc taacccaatt tactacatta ttgtctgtgg ttttcataaa tagtacaata
     5821 atcatccatt ttcttgaaac aaacatcaaa tacaataccg atatactaat actgaacttt
     5881 tcttgactaa aaacagctat attataatcg tatcatgagt ttttatggtt ttatggtaaa
     5941 taaaaatgga gctgtaacat atatgaaaaa aatagtaaat atcgggcttg tgggttttgg
     6001 aactgttgga agcggcgttt acaacgtcat aaagaacaac ggtaaactga tcgagcagag
     6061 aacaggagtt agccttgttc tcaaaactgt ttgtgattta agaatggatg aggttaagaa
     6121 taccgtttca tctgatgttc aagtgacgga taactggaaa agtctgattg aagataaaga
     6181 gatagataca attgtagagc ttattggcgg tcttgaaccg gcaaagagcg ttgtcgaagc
     6241 cgcgctttcg gcaggtaaaa acgttgtaac tgcgaataaa aaacttttgg ccgaaaaggg
     6301 ttccgtaatc ttcgaactgg ccgatcgtaa ggatgccaaa ctggggtttg aggcggcagt
     6361 aggtggcggt atcccctgta tcgaggatct gaaaaacggt cttgtggcta acaatgttaa
     6421 tcatattttt ggtatcctta atggtacaac caattatatt cttacccgta tggaacttgc
     6481 cggattatct tttgccgatg ccttggccga ggcacaggcc gaaggttttg ctgaggctga
     6541 tccgactttt gatattgaag gatacgatgc gggccataag atttgtctgt tggccatgct
     6601 tgcgttcaat aaaaagatag agtacggctc aatccagata gagggtataa caaaaattaa
     6661 aactctggat accgagtttg cccgtcgcat gggatacgcg gttaaacttt tgggaattgc
     6721 ccgtgaaatt gatggtgagg ttgatgtgcg ggtgcaccct gtcatgattc gggaatcaca
     6781 cctgattgcc tcagtaagtc atgagtataa tgctgttgtt tttgacgcgg atatgacagg
     6841 agaagttctt ctttatggaa agggtgccgg ttctttgcct acggcatcgg cagttgtttc
     6901 tgatattgtt cagatagctg ttaaaaaagg agtatatgaa agcgctattt taacaaaagg
     6961 agatgctgtt atacttggtg cggaaaaacg attttcaagg ttttatttac gtattaatac
     7021 cgaagaccgg ccaggtattc ttgaaaaggt tgctcacgcg ttttcggcaa ataatatatc
     7081 tgtaagctcc gttgaacagc atgaaacagg ccataatata gtgcctcttc ttttcgttac
     7141 ccacaaggcc tgtgaagctg atattagaaa ggctgttaat gaaataaatg agttcgattt
     7201 tgtaaccgaa cctgtaatga tgataagaat cgaagagagt tgatactaat tcttgaatta
     7261 ataaattgtt taatatataa caattgtgtt gtttgtaaaa ataaaatagc tgtccgaggt
     7321 taataaacgt gaactcttat tttaagtgta taaaaggctg tgaggggcaa tactcattga
     7381 ccgatattat ttaccgttgt cccaagtgtg gttctcttct acaggttacc cacgataccg
     7441 atgctcttaa ggcaaagtta aatggagaag ggtggaacaa actttttgca tcaagaatgg
     7501 ggagcacaaa atggccctac ggttcaggtg tatggcgtta taaggagctg gttgctcctg
     7561 aaattgatga tgattccatt gtttcgatgt acgaaggaaa cagtaaccta ttctatgcga
     7621 aacgcttcgg acagatgttt ggcgttaagg atctttggat caagatgtgt ggaaacaccc
     7681 acaccggatc gtttaaagat cttggtatga ctgttcttgt ttcgatggta aaccagatga
     7741 ttaaaaaagg taaaaatata aaggctgtcg cttgcgcgtc aaccggtgat acctctgcgg
     7801 ctcttgcggc ttatgcggct tatgcaggaa ttccatcgat agtgtttctt cctgccaaca
     7861 aaattacaac agcgcagctg gttcagccca tgtcaaatgg ttctatcgtt ttggcgttgg
     7921 ataccgactt tgacgggtgt atgaaaatag ttcaacaggt taccgagaac aacgaaattt
     7981 atcttgccaa ctctatgaac agccttcgtg tcgaggggca aaagacaata gctcctgaaa
     8041 tagtacagca gtttgatatg gaagttcctg atacaattat cattccgggt ggtaatttgg
     8101 gtaacgtgtc tgctttggga aaaggcttca aagagatgaa agaacttggt atgataagca
     8161 agatgccaag aattgtcgtt gctcaggctc agaatgcaaa tccgctctat acttcttata
     8221 aaaatggttt tgaaagtttt tcatcaatat ctgcgaaaaa gactcttgct tcggcaattc
     8281 agataggcga cccggtttca gtagacaagg ctatcgccac gttaaaagag tttaatggaa
     8341 ttgtagagca ggctagcgaa gaggaacttg cagaggctgc ggccttggca gatagaaccg
     8401 gtatgtacag ttgtcctcat acaggtgttg ccttgtcagt ttttacaaaa cttgcctctc
     8461 gtggcgatat cgcatctgat gagagagtag ttataatatc tactgcgcac ggtttaaaat
     8521 tttccgagtt taaagtaggt tatcatgaag aaacaattag cggtataaac tgttcccatg
     8581 caaataaacc gatagagctt gctgccgata gcgatctggt tttgaaggta cttgaagaag
     8641 aaataaaaaa attgaaatga aaattttaca ggtagcttga ttgcgtgttt gccttgcgat
     8701 aattttaaga tacctgccgg ataaatatta aaaaagtaag gaatatatta tgacaaaagt
     8761 acatactcct gtaaccgata aaatggattt ttttaaagag tcaactcctg ttcaactggc
     8821 agaaaaatac ggaacacctc tttatgtgta taacgaaaac attctgcgag aacgctgtcg
     8881 cgaaatgaaa ggcttggtag attataaaaa cttttctgtt aactactccg ctaaggcaaa
     8941 cagcaatctt gagcttctta aaattgttaa cgaagagggt ttgtctgtgg atgctatgag
     9001 ccctggtgaa attcatgctg aacttgccgc gggatttact gcggatagaa tcttgtatat
     9061 aagcaataat gtctccgccg atgagatgcg tttttctgta gaccggggaa taatggtgag
     9121 tattgattcg ctatcacagt tggatcttta tggccgaaac tttccaggaa caaaggtcgc
     9181 tctcaggttt aatccgggcg tgggggctgg acatcatgaa aaagttgtta ctgcaggtaa
     9241 aaagaccaag tttggtattg ctcccgagat gatgtcccaa gtcaaagaga tagtcaagaa
     9301 acatgatctg acagttgccg gagtgaatca gcatattggc tcactcttta tggaatcgga
     9361 tgcttatgtg aagagtacag attccattct ttcgattgcg tctaattttg attcacttga
     9421 gtttgtcgat tttggtggag gctttggtat accttatcgt aagtctgaag ggcagaaacg
     9481 tcttgatctt gccgaaactg gacgtttgct taagcaaaat attgacaagt ttatcaaagc
     9541 ctatggccgc gagataaaaa tcaaaattga acctggacgt tatatcgcgg cagaatgtgg
     9601 cataattctg ggcagggtac actctgttaa ggaaaactac ggatcacgtt atataggaac
     9661 cgatatcggt tttaatgtgc ttaaacgccc ggttatgtat gattctcatc atgaaataga
     9721 gatttttcgt gaaaaaggta gcatcgatag ctcaaaagat tgtgtaaaaa ttgtgggtaa
     9781 tatctgtgaa actggagata tcctcgcgaa cgatagggag cttcctaaaa tagttgaaga
     9841 tgatattatc gcggttcttg acgctggggc ttacggtttt tgcatgagtt ccaactataa
     9901 taacaggtta aagcctgccg aggtattaat ttcgtccgat ggcagtgaca aattgatccg
     9961 cagaagagac actcttgagg atctgatgag aaattttgaa ttataagata ctttaactgt
    10021 atcagtttaa ttgcagtttt tgccgatact atgtataagt cttgagccga tttaattcta
    10081 aacggatgta ataatgaaca aaaagataat tgttgtagac gatgacccgg taaaccgaac
    10141 attgatatgt tcggtgttaa aaaaatacac cttgtttacc gcggaagata taaaatcgat
    10201 gtacaatatt attaaggaga acggtcttcc tgatctgata ctccttgacg ttatgctgcc
    10261 tgaagtaaac ggtttcgagg ccgcaagaga tttacataca gagtttagaa atcaggacgt
    10321 aggtatcatt tttgtaagcg cgcggaccga aggctcatcg gttaaagagg gcttcgattc
    10381 gggtggttat gattacatca aaaagccttt tgattccatc gagcttgtgg cgcgggtaga
    10441 aaaccagcta gagcgtattg aacgtgaaaa caagctacgt tctgatgctt cacttgaccc
    10501 tttaacagcg gtttataatc gccgatattt caaacatctt gtagagcaga agattaattt
    10561 ctgtaatcgt aaaaatatga aactctctat cgctatgata gatcttgata atttcaagga
    10621 aattaacgat aatttttctc atcttgccgg tgactatgtt ttgcagacct ttgctagcca
    10681 gatatcagag agccttagaa aatatgatct tatgggaagg tttggaggag aagagtttat
    10741 tatcgctttt gttgactgct gcaaagattg ctcttattcg ataatggagc gtcttcgtgc
    10801 cgatgtcgaa aagagagcat ttatctttga aggaaatgat attagtctat cgtttagtgc
    10861 tggtatagcc gatttgtgcg aaatcgaaag ccgaaaaagc gtttacaatg aaattctgaa
    10921 attatcagat cagcggttat acgaggcaaa gcgtcttggg cgcaaccaga tagtcatata
    10981 accgcttaag tgtgaataaa agtaattagg ttagtctgcc gaaagacctg tgtgatccct
    11041 tagcacagtc tctatgaaat caatgctttt gtctccaaac tctgcagatc gctcttgaag
    11101 tgaatatcta tcaacaatat tatcccgtat ctgttttgca taatcttctg ctacagtctt
    11161 gtttccaccg tttgatacaa aagtaataat cttctgtatt gtagaactct cagtcgaagt
    11221 aagacatggc gtttcaagaa cactaggttt gaaagtacta cccgctgtta caggagcctt
    11281 cgccgggaga tcttcgatta ctgctgtttt agctgcaata tctccaatgc gttgcccgct
    11341 ctctttaaaa gcgattgtaa gaatcgcagg taatccgctt gaaatatgca ggtctattat
    11401 tctcatggtc cagcgtatta ggcattgacc aattgataga ggcgatccat ccagggatat
    11461 aacgcgaatc ttcattatgt ttttgccgat ggtctgtccc ttcattccta tttcgcaaag
    11521 caaattgtac agcataagcg gtaaggctat aaaaaaaagc ggaagaagag tggatggctg
    11581 attaaaattg attttatcaa aatttaattc tgaagttatt aataataaaa ccatgatgaa
    11641 tattccggta aacaataata aggcagaatc gataccccat gccaatattc gtgaacccaa
    11701 gggtgctgct ttgtaggtta tatttacatt atggactgtg gtta
//
LOCUS       DAOTGZ010000073        11544 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_3064
            NODE_6649_length_11544_cov_8.673166, whole genome shotgun sequence.
ACCESSION   DAOTGZ010000073 DAOTGZ010000000
VERSION     DAOTGZ010000073.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868088
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 11544)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 11544)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:22:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,785
            CDSs (total)                      :: 2,751
            Genes (coding)                    :: 2,732
            CDSs (with protein)               :: 2,732
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 12 of 19
            Pseudo Genes (internal stop)      :: 5 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11544
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6649_length_11544_cov_8.673166"
                     /isolate="AMR_MDS_3064"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..445)
                     /locus_tag="P5123_06420"
     CDS             complement(<1..445)
                     /locus_tag="P5123_06420"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRX15929.1"
                     /translation="MKRYVVLLFLCTLFVSVALAQTEEIKQKDGTRIVLDKKKGIKTF
                     YYGDGRIQKVDLNGKTPYGLAIENVKVLIQDDPIRVDLQYSAVYSDELLIENAEKFFS
                     KLASVLKNKKYIGKKPVDIVVSYCRYGMYGYCLDKQNVITVFITEN"
     gene            complement(520..1314)
                     /locus_tag="P5123_06425"
     CDS             complement(520..1314)
                     /locus_tag="P5123_06425"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRX15930.1"
                     /translation="MKGFFRNLALQKDLLLSITGLCLVLFIVAHLAMDATIIIGPDAF
                     NGIATILERFYLAQIGVPLVILALVTHIVVALLKQKNKFLSVSKAFVRATHPETLIWI
                     IQLTTGLLLIGLALLHVVVIVLDNVTKINIVSTSVRIADVKFQIFYHVLMNVTMVHIS
                     CGIYRLYAKWVGSGRRKFIPFVLLFFLIYWSVSTVALQKYMDYAPHFKKLKSLLIDYE
                     KDRENDVLRHKIIKISLEQNISITPELLKKIGIDKDILPDFSNTGH"
     gene            complement(1320..1955)
                     /locus_tag="P5123_06430"
     CDS             complement(1320..1955)
                     /locus_tag="P5123_06430"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRX15931.1"
                     /translation="MDYRLVKQFLNDAIDNFFNFFYKLIDFGKVIIDTFWAFMEIWYN
                     FFMFFINIFLYGYYLLLFIIDRMTMSSAPIFFWRTGGRATRQEFKRAYDRNLVIPVSG
                     AYGKGSSRSAVASAASSVSSAVSSGASSVSGAAASSVAMGKYSAKPNIIIRFFALIGR
                     AIVAVIKGIGGAFCAIGRFFTSRLVPVKEKPVGRKNLVEDYLKQYDSRKSR"
     gene            complement(2089..2637)
                     /locus_tag="P5123_06435"
     CDS             complement(2089..2637)
                     /locus_tag="P5123_06435"
                     /inference="COORDINATES: protein motif:HMM:TIGR01383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DJ-1/PfpI family protein"
                     /protein_id="HRX15932.1"
                     /translation="MKTAIILAEGFEDIEAITTIDVLRRAGISIDVVSAASELVKGAR
                     GIIVKSEKNIDEIDSSEYACVVCPGGLPGSDNLMNDERVIGFVQQVYDKGGIAAAICA
                     APQVLNRAGLLKGKKVTCYPGREKSFDESVIYTKEQFTIDGRIITADGPGSAMIFALE
                     LVALLAGREKSETVRLGLRYNL"
     gene            complement(2661..3194)
                     /gene="pth"
                     /locus_tag="P5123_06440"
     CDS             complement(2661..3194)
                     /gene="pth"
                     /locus_tag="P5123_06440"
                     /EC_number="3.1.1.29"
                     /inference="COORDINATES: protein motif:HMM:NF013368.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004045 - aminoacyl-tRNA hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminoacyl-tRNA hydrolase"
                     /protein_id="HRX15933.1"
                     /translation="MIIIACLGNPGTKYRFNRHNCGFLTAYYLAEKNGADISKKQFNA
                     LTAKFRIAEKEVMLVLPQTYMNLSGESVSAALKYYDLDHRSLIVIHDELELPFGDIRE
                     KKGGGHKGHNGIRSIIASIGSAEFSRVRFGIGRPPSDKIPVASYVLSDFSPEEKAELE
                     DCYKKASSLIEEFCIAN"
     gene            complement(3304..3645)
                     /locus_tag="P5123_06445"
     CDS             complement(3304..3645)
                     /locus_tag="P5123_06445"
                     /inference="COORDINATES: protein motif:HMM:TIGR00377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043856 - anti-sigma factor antagonist
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="STAS domain-containing protein"
                     /protein_id="HRX15934.1"
                     /translation="MDYKINRENETCLVDLNGSLSNDLIDDIKNVLIKEVNESKKGLI
                     VSLENVSFLCSAAFGMFFALHTLAQKSGKKICFCCVKQEIEKLFAITGVHRHMNIAKT
                     RDEALSFMKSY"
     gene            3884..4873
                     /locus_tag="P5123_06450"
     CDS             3884..4873
                     /locus_tag="P5123_06450"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: protein motif:HMM:NF025010.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HRX15935.1"
                     /translation="MKITKIILLLVISITAFSSITADNRKWYTVDKVVALVNDDPILY
                     SDLYKRVEINKNRKGITAKDKKNMLYVTLDSYINELLLMQAANREYIIVSDEKVDNRI
                     KEVMEHTGIKDIEQFKKNIEREEKIPFEVYREEMRKQITMELIMVYAMTFTPPSEAEA
                     KAWYEKNKKQLLQLRFQHILIIPKGKSFSAEKEANELASQLRERAMSGESFARLARQY
                     SDDKASARADGFIDWISPGQLDQRFAYHVFNMKSRGEISSVFKSSFGYHVVKYYGRRY
                     LPYDQIRDRIFQMISQEARMNQFTEWVKGERANSHISVYVDGFDKYSASQDKK"
     gene            4870..6351
                     /locus_tag="P5123_06455"
     CDS             4870..6351
                     /locus_tag="P5123_06455"
                     /inference="COORDINATES: protein motif:HMM:TIGR04321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="spiro-SPASM protein"
                     /protein_id="HRX15936.1"
                     /translation="MNNAFVLYLEKHTDDDDLNFNGSKTFDIIKEKINSAFADTEIIA
                     SLPEAYTGDLDKFSKVYVRSDNETENWKNLNEQFSFDNYIRIKADAPFCDMQIVGEML
                     DVHTKYIAEYTYSENIPAGFACDILSSELIKSLPDSSENGKSILPVEKVIKDNINQFD
                     VELFYKEPDIRAYRLSFLSGDKRENMIMQNMIKVADNISYDSVEKVLTENPDVLYTTP
                     SYIEIELSPETNANPLYSPITLAKKERSQMDSELFDKIITELDLFKPPVALSLSGFGE
                     PLKHSEFYSIFEKALNNTNISILLLETDGLALDQNFMNFIQNRDVSKLRVIVECNGYS
                     AETYHSIHNLDEFETVNKNILNLAPVLKENLYVQIMKINETEPFLDAYYDYWEGHKVQ
                     IILQKQNTYCGRLTDRRYYDLTPLKRIPCWHLLRDLLILSDGSIPFCKQDPGAEFSLL
                     SAKDMPILKIWEERKPLFVDNFNKKYAAKPDCKSCDEWFTFNL"
     gene            6375..7136
                     /locus_tag="P5123_06460"
     CDS             6375..7136
                     /locus_tag="P5123_06460"
                     /inference="COORDINATES: protein motif:HMM:NF014409.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRX15937.1"
                     /translation="MKKTENSVNTIAVIQARQNSKRLHEKCFKTLMGKAVLSHIIKRA
                     QAIEGVSQVIVATGDANANKDIIALAESEGAVALPGSETNVLERYYNASQLYDCDYIV
                     RITGDNPFTDVDYANMAIEYAIETDADLASVTNIPLGTAVEVIKKEALADCYQNASEA
                     HHFEHVTPYIKENEHLFNICRKPVYIYNNIPDLRLTIDTKEDYELACELYNALYNEDS
                     IFSLHQILNHLRLFPDMALINSEIKQRPMTHCENG"
     gene            7123..8004
                     /locus_tag="P5123_06465"
     CDS             7123..8004
                     /locus_tag="P5123_06465"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRX15938.1"
                     /translation="MKTADICIMTRCGSDYGSGHFWRMTNLLSILRNQGLDVYISCDC
                     YPDNFPDNLRPYTSHSPVKAEILIRDMRDSTKDAASLLAAKYGRIISIDDAGYTSLYN
                     KNIDLLPRAEHPIETGDNNFPFIFGYHFSKAINGLHSTPEKDLDLLIYTGFDNNDYPL
                     LLENQSLEIAFFNGSNCYYKGSTLSGSSYYNLLFKSKNFLSHFGLSLYEAYICNCNIF
                     ALNPSDYHSKLADNEKRIPVINLGTFANINKELQNTLKKTILEKTVLSMFNLEETKNR
                     INEMQSLFVKLLEITIN"
     gene            complement(8008..8601)
                     /locus_tag="P5123_06470"
     CDS             complement(8008..8601)
                     /locus_tag="P5123_06470"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRX15939.1"
                     /translation="MKKYLVIVLIVFCTFEIFANEDEVFKRGDTQISFGLAATSGSDY
                     SLYYLNGGYGYFLTNRLVLGPAIGLYVTSDSDIIYEPMATARFYIMNRRPAMPFIGVN
                     TGYIYSPNYTYRTSYTVFCDASIDATFAENEFESELSILRLSPEAGVSYLVSKHAVAT
                     LTVSMDMFYPMNEEPEKGYKLEETGSLSVKVGFSMML"
     gene            complement(8710..9120)
                     /locus_tag="P5123_06475"
     CDS             complement(8710..9120)
                     /locus_tag="P5123_06475"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3795 domain-containing protein"
                     /protein_id="HRX15940.1"
                     /translation="MKAKPKTNAEDLANSIAYCGLICKLCFLKDSCDGCKSKNNLCDR
                     NLADKGCFQKQCCTENNYEGCWECESLYSCTEGIYSLGDYSKIKAFAICIREDGKDKF
                     INYILENTKKGWSVEKDKDYDGKKIDEVLEMLRG"
     gene            complement(9386..9667)
                     /locus_tag="P5123_06480"
     CDS             complement(9386..9667)
                     /locus_tag="P5123_06480"
                     /inference="COORDINATES: protein motif:HMM:NF019421.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HipA domain-containing protein"
                     /protein_id="HRX15941.1"
                     /translation="MSFSETRLFSFKRCDGYFGVNRFDRIYNKKIYMISVSGVLESSH
                     RYPNFDYHLLMKLTFEPTGIFEEVEKMYSFVCFNVFVHKRDDHSKIFLI"
     gene            complement(9814..10698)
                     /locus_tag="P5123_06485"
     CDS             complement(9814..10698)
                     /locus_tag="P5123_06485"
                     /inference="COORDINATES: protein motif:HMM:NF012363.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HRX15942.1"
                     /translation="MKFFKILILISVLPFISCSKGPSSLVNKIVSDSGVPEKRVYKEF
                     HSDKFAMDKLEVHIFYPEGYDVNREEPYKVAAYFHGGGWSQGSPTEGYPYAKHFVSLG
                     FVAVSFEYRLVDYVNVTGVECVKDVNSAIRWLRTYNKELNIDPDHIATVGLSAGGHLV
                     IANSMFPDIIEEGEDPKISSIPNIVWAFAPPVSFKNNNFYMSVALDTEQVLQICPMTN
                     IKPESVPVYIIHGNRDSLVYYDDSVLFVKKMQEAGNESELWTIREGKHSSFWENQEHM
                     DYVYTVINDQMKKIKWIE"
     gene            complement(11088..>11544)
                     /locus_tag="P5123_06490"
     CDS             complement(11088..>11544)
                     /locus_tag="P5123_06490"
                     /inference="COORDINATES: protein motif:HMM:NF013045.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="RNA pseudouridine synthase"
                     /protein_id="HRX15943.1"
                     /translation="QERISQQIKAGMLYKEYRGIVEGILEKTEGIISSCISRADDGTI
                     KRVCGGKGKESVTQYQVLSNNGNFSELKFIMHTGRTHQIRVHMAMIGHPIAGDEMYGF
                     ASEDMRRQALYCSCLKFTHPLNNNVIEIRVPMPNDIAQFYGSIKTQKEI"
BASE COUNT         3761 a         2319 c         1879 g         3585 t
ORIGIN      
        1 tattctcggt aataaatact gttataacat tttgtttgtc cagacagtaa ccatacattc
       61 cgtaacggca ataagagact actatatcca ctggtttctt gccgatatat tttttgtttt
      121 ttaagacact ggcaagtttg ctaaaaaatt tttctgcatt ttctattaaa agttcatccg
      181 aataaaccgc gctatattgc aaatcgacac gtattggatc atcctgtatt aacactttaa
      241 cattttcgat agcaagacca taaggagttt ttccgtttaa atcgactttc tgaattcgcc
      301 catcaccgta ataaaatgtt tttatcccct tctttttatc cagcactatc ctggttccgt
      361 ctttctgttt aatttcttct gtttgcgcta atgccacaga gacaaagagt gtacataaaa
      421 acagcaacac aacatatctt ttcattaaag ataaccctta acaataaaat ctacttccaa
      481 gtaacactat cggatttttt taacacaaag taaacataat caatgccccg tgttcgagaa
      541 atcaggcaaa atatctttat caataccgat tttttttagt aattcaggtg taatggaaat
      601 attctgttcc agagaaattt ttataatttt atgtctaaga acgtcgtttt ctcgatcttt
      661 ttcataatca atcagtagac ttttcaattt tttaaagtga ggagcataat ccatatattt
      721 ttgcagagcg accgtactga cactccagta aatcaaaaaa aagaggagaa caaaaggtat
      781 aaattttctt ctgccggatc ctacccattt ggcatataaa cggtatattc cacatgagat
      841 atgaaccatc gttacattca ttaatacatg ataaaatatc tgaaatttga catcagcaat
      901 tcttactgac gttgatacta tatttatttt tgttacatta tccaacacaa taaccacaac
      961 gtgaagaaga gcaagaccta ttagtaaaag tcccgttgtt aattgaatta tccagattaa
     1021 tgtttcggga tgtgtagctc ggacaaatgc cttggataca gacaagaact tgtttttttg
     1081 ttttagcaga gcaactacta tgtgggtaac taaagccaaa attaccagcg gaacaccgat
     1141 ttgtgccaaa taaaatcttt ccagaatagt agcgattccg ttaaaggcgt caggccctat
     1201 aattattgtg gcatccatag ccaagtgagc aactataaac agaacaagac agagccctgt
     1261 aatggaaaga agcagatcct tttgaagcgc aaggtttcga aaaaaacctt tcatcttgat
     1321 taacggcttt ttcggctatc gtattgttta agataatctt ccacaaggtt tttacgccca
     1381 accggcttct ccttaacagg aacaagtcgc gaggtaaaaa accttccgat tgcgcaaaat
     1441 gctccgccga ttccctttat tacagcaacg attgctcttc caattaaggc aaaaaagcga
     1501 ataatgatat ttggttttgc agagtatttg cccattgcaa cggatgaagc ggctgcaccc
     1561 gaaacagaag atgctccaga ggataccgcc gaagaaacag aagatgctgc agacgcaact
     1621 gcggatctgg aagaaccttt tccgtatgca cctgataccg gaataaccag gttacgatca
     1681 taggcccgct tgaattcctg acgagtagca cgcccccctg ttctccagaa gaaaatggga
     1741 gctgaagaca ttgtcatgcg atcaataata aaaagaagca gataatatcc atacaaaaag
     1801 atattaataa aaaacatgaa gaagttatac catatctcca tgaaagccca gaaagtatcg
     1861 attattactt ttccaaagtc tataagctta tagaaaaagt taaaaaagtt atcaatagcg
     1921 tcattcaaaa actgttttac cagacgataa tccatattac ctcgttacaa acaatttatc
     1981 tgtgttaata ttatagcccc ttaggacaga ttaagctaat ataattcatt atcgagtcaa
     2041 taacattttt tggcatctta agcccggtga ggcttaagga ttatgtggtc agagattata
     2101 tctgagcccc aatcttacag tttcggactt ttcgcgacca gcaagcaacg cgaccagttc
     2161 cagtgcaaaa atcattgcac tgccaggacc atctgcggta attattcttc cgtcaatagt
     2221 aaactgttct tttgtataaa ttacggactc atcgaaactc ttttccctgc cgggatagca
     2281 tgtaactttt ttgcccttta aaagaccggc cctattaaga acttgaggcg ctgcgcaaat
     2341 tgcagcagct attcctcctt tatcataaac ctgctgcaca aaaccgatca ctcgttcatc
     2401 attcatcagg ttgtcactac caggcaaacc tccaggacaa acaacacagg catattcgga
     2461 agaatcaatt tcatcaatat ttttttcact ctttactata ataccacggg ctcccttaac
     2521 aagctcggaa gcggcagaga ctacgtcgat actgatccca gcccttcgta aaacatcaat
     2581 tgtggttatt gcttcgatat cctcaaaacc ttctgctaaa ataatagctg ttttcatgat
     2641 accccctttt aaataattga ttagttcgct atacaaaatt cttcaattaa actggaagct
     2701 tttttataac aatcttcaag ttcagctttt tcttccgggc taaaatctga taaaacataa
     2761 gaagccacag gtatcttatc cgaaggcggc ctacctattc caaaacgaac tcgggaaaac
     2821 tcggcgctac ctattgaagc aataatggaa cgaattccat tatgaccttt gtggcctccc
     2881 cccttttttt cccgaatatc tccaaaaggc agctcaagct catcatgaat tactatcagg
     2941 ctacggtgat cgagatcgta atattttaat gctgccgaaa cagactctcc gcttagattc
     3001 atatatgtct gcggcaaaac aagcatcact tctttttctg caattctgaa ctttgctgtt
     3061 aaggcattaa actgtttttt tgatatatca gcaccatttt tttccgccag atagtaagct
     3121 gtcaaaaacc cgcaattatg tctgttaaac cggtattttg ttcccggatt ccctaagcac
     3181 gcgataatta tcatataaat ttccaattat tttccaatga tatataaaca aaaaaagagc
     3241 ttctccaaag aaccggaaac tctttatact aagtcataac aattaaataa aatcagataa
     3301 aaactaatag gatttcataa aagacaaggc ctcatctctt gttttagcta tattcatatg
     3361 tcgatgtaca cctgttatag caaaaagctt ttcgatctcc tgcttgacac agcaaaaaca
     3421 tatttttttt ccactttttt gagcaagagt atgaagagca aaaaacatcc cgaaagccgc
     3481 gctgcacaaa aaagagacat tttccaagct tacaataagc cctttttttg attcattaac
     3541 ttctttaatt aaaacatttt taatatcatc aattaagtca tttgacaatg atccattaag
     3601 atctacaaga caggtctcat tttctctgtt tatcttataa tccattgcct actcccatac
     3661 ataatattat agtttatctc tttttttagt caagagattt ctttaaagcc gttttttatt
     3721 aacgttattt taaaacttct attttcaaag tttgggatta taaaaaaaac ttgcgttatt
     3781 ttgcttaaat aggttattaa gatgttttac gtattaacca gtcatagttc aaataaagac
     3841 tacaataagt aacaaaaaca actataaaca accgaagagt attatgaaaa ttacaaaaat
     3901 tattttatta ttagttatat ccattacggc tttttcttct attacagcgg ataatcgaaa
     3961 atggtacacc gtggacaaag tcgtagctct tgtaaatgat gacccgatac tgtacagcga
     4021 tctgtacaaa agagtcgaaa taaacaaaaa ccgcaaaggt attacagcta aagacaagaa
     4081 aaacatgctc tacgttaccc tagattcata tataaatgaa cttcttttaa tgcaggcagc
     4141 aaaccgtgaa tacattatag ttagcgatga gaaagttgac aaccgcatta aagaggttat
     4201 ggagcatacc ggaataaagg atattgagca atttaaaaag aatattgaac gcgaggaaaa
     4261 gatacctttt gaagtatacc gcgaagagat gagaaagcag atcacaatgg agctgatcat
     4321 ggtatacgcg atgacattta cccctccatc tgaggctgag gcgaaggctt ggtacgaaaa
     4381 aaacaaaaaa caactgcttc aactccgttt tcagcatata ctaattattc caaaaggaaa
     4441 atcgttttct gccgaaaaag aggcaaatga actagcatcc caattaagag aacgagccat
     4501 gagtggcgaa tcttttgcca gacttgccag acaatattct gatgacaagg cttccgccag
     4561 agctgacggt tttattgact ggatttcgcc gggacaactt gatcagcgct ttgcctatca
     4621 tgtctttaat atgaaatccc ggggagaaat atcttctgtt ttcaagtcct cttttggata
     4681 ccatgttgta aaatattatg ggcgccgcta tttgccttac gatcaaattc gtgatagaat
     4741 tttccagatg atatcgcagg aggcccgaat gaaccagttt acagaatggg ttaaaggtga
     4801 acgagccaat tctcatatta gcgtttatgt tgatggtttt gacaaatata gcgcatccca
     4861 ggataaaaaa tgaataacgc ttttgtttta tacttagaaa aacatacaga tgatgatgat
     4921 cttaacttta atggttccaa gacctttgac attattaaag aaaaaattaa cagtgctttt
     4981 gccgataccg agattattgc ttcattaccg gaagcttata caggagatct tgacaaattt
     5041 tcaaaagttt atgtaagaag cgacaacgaa acagaaaact ggaaaaacct caacgagcag
     5101 ttttcatttg ataactatat aagaataaaa gctgatgctc ctttctgcga tatgcagata
     5161 gtaggtgaaa tgctcgatgt tcatacaaaa tatatagccg aatataccta ttcagaaaac
     5221 attccagctg gttttgcctg cgacattctc tcttccgagc ttataaaatc tcttcctgat
     5281 tcaagtgaaa acggaaaatc aatactgcct gtagaaaaag ttattaaaga taatattaat
     5341 cagtttgatg tagagctttt ttacaaggaa ccagatatac gggcttaccg actctctttt
     5401 ttaagcggag acaaacgaga aaacatgatc atgcaaaaca tgattaaagt tgctgataat
     5461 atttcgtatg attccgtaga gaaagtactc actgaaaacc cagatgtgct ttatacaaca
     5521 ccttcataca ttgaaataga gctttcacct gagacaaatg ccaatccgct atattcacca
     5581 attactcttg ccaaaaaaga acgtagtcaa atggatagcg agctttttga taaaattatt
     5641 acagagctag atctgtttaa gccccccgtt gctttaagcc tttcaggttt tggagagcct
     5701 cttaaacaca gcgaatttta ttctattttt gaaaaagcgc tgaataatac taatatttct
     5761 attctgctct tagaaaccga tggccttgct cttgatcaga attttatgaa ttttatccaa
     5821 aatcgtgatg tatcaaaact gcgggtgatt gtagagtgca acggatattc tgccgaaaca
     5881 tatcattcta tacacaatct tgacgaattc gaaacagtta acaaaaacat tcttaatctt
     5941 gcacctgtct tgaaagaaaa cctgtatgta caaataatga agattaacga aaccgagcca
     6001 tttcttgacg catactatga ctattgggaa ggtcataagg ttcaaattat acttcaaaaa
     6061 cagaacacct attgtggtcg ccttactgac cgccgctact atgaccttac accactaaaa
     6121 cgaattccat gctggcacct gttaagggat ctgcttatac tttcagatgg atctatcccc
     6181 ttttgcaaac aggatcccgg tgccgaattt tccttattat ccgcaaaaga tatgccgatt
     6241 ttaaaaatat gggaagagcg taagcctctg tttgtggata attttaataa aaaatatgct
     6301 gccaagcccg actgtaaatc ctgtgacgaa tggtttacgt ttaatcttta agcaattcaa
     6361 aaaaggaaag cattatgaaa aaaactgaaa actcagttaa taccattgca gttatacagg
     6421 ctcgtcaaaa ctcaaaaagg cttcatgaga agtgttttaa aacacttatg ggtaaggctg
     6481 tactttcaca tataattaaa cgagcgcagg cgattgaagg tgtttctcag gttatagttg
     6541 caacaggaga cgcaaacgcc aacaaagata ttatcgctct tgccgaatct gagggggcag
     6601 tggctttgcc tggatcagaa actaatgttc ttgagagata ctataatgct tctcaattat
     6661 atgattgtga ttacatagtt cgcataactg gggacaatcc ctttacagat gtagattacg
     6721 ccaatatggc tattgaatac gctatcgaaa ccgatgccga tcttgcttct gtcacaaata
     6781 ttccgttggg tacagcagtc gaagtaataa agaaagaagc tttagctgat tgttatcaaa
     6841 acgcgtctga agctcaccat tttgagcatg taacaccata tattaaggaa aatgagcatc
     6901 tttttaatat ctgccgcaaa cctgtctata tttataacaa tattcccgat cttcgtctta
     6961 ctatagatac taaagaagat tatgagctag catgtgaact ttataatgct ctttacaacg
     7021 aagattccat tttttcatta catcagatat taaatcatct taggcttttt cctgacatgg
     7081 ctcttatcaa tagcgaaata aaacagcgcc caatgacaca ctgtgaaaac ggctgatatc
     7141 tgcataatga cccgttgcgg ttctgattat ggcagcggac acttttggag aatgaccaac
     7201 ctattatcga ttctcagaaa tcaaggtctt gatgtttata tcagctgtga ctgctatccc
     7261 gacaactttc cagacaatct gagaccttac acctcacact cgcctgtaaa agccgaaatt
     7321 ctaatccgtg atatgagaga ttccacaaaa gatgcggctt cacttttagc tgcgaagtat
     7381 ggaagaatca tttcaattga tgatgcaggt tacacatctc tttataacaa gaatatagat
     7441 cttttgccga gagccgaaca tcctatcgaa acaggtgata acaattttcc gtttattttt
     7501 ggttaccact ttagtaaagc tattaacggt ttgcactcta cacctgaaaa agatctggat
     7561 cttctaattt acaccggttt tgacaataac gattatcccc tcttgttaga aaatcaaagt
     7621 ttagaaattg ctttttttaa cggttcaaac tgttattaca aagggtcaac gctaagtggt
     7681 tcaagctatt ataatttgct ctttaaaagt aaaaactttc tttcacattt tggactatct
     7741 ttatacgaag cctatatatg taactgcaat atcttcgcac tgaacccttc agattatcac
     7801 tctaaattgg ctgacaacga aaaaagaata cctgttatta atcttggaac ttttgccaac
     7861 attaacaaag agctacagaa tactcttaaa aaaacaattc tggaaaaaac tgttttatca
     7921 atgtttaatc tggaagaaac aaaaaacagg attaatgaaa tgcagtccct ttttgttaaa
     7981 cttttagaaa tcactatcaa ttagttttta tagcatcatg gaaaaaccca ctttaaccga
     8041 aagtgaccct gtttcttcta acttgtatcc tttttcaggt tcttcattca taggataaaa
     8101 catatccata gaaactgtta aggttgcaac tgcatgtttc gataccagat acgacacccc
     8161 agcctctggt gataatcgca aaatagaaag ttcagattcg aattcatttt cagcaaaagt
     8221 cgcatcaata gaggcatcac aaaaaaccgt ataagatgtt cgataggtat aattaggcga
     8281 atagatatac ccagtattaa cccctataaa cggcatcgca ggtcttcggt tcattatata
     8341 aaatcgggcc gttgccattg gttcatatat tatatcgcta tcagatgtaa catataaccc
     8401 tatagctgga ccaagtacta aacggtttgt taaaaaatat ccgtagcctc cgtttaaata
     8461 atagagagaa taatctgacc cagatgttgc ggcaaggcca aaagaaattt gtgtgtctcc
     8521 tctcttgaaa acttcatctt cgtttgcaaa aatctcaaat gtacaaaaga caatcagtac
     8581 tataaccaaa tattttttca tatcatctcc tattctgtcg aaaagaggcc aatttttatt
     8641 gtatttttgc ataatattta aaacaacaga ggtttgtcaa tcattcaaat gttttactct
     8701 tctatgtaat tatcctctta gcatttcaag tacctcatca atttttttgc catcgtaatc
     8761 tttatctttt tcgacgctcc accccttttt tgtgttttca agaatatagt tgataaactt
     8821 gtccttgccg tcttctctta tgcaaatagc aaatgccttg atttttgaat aatcacctaa
     8881 cgaataaata ccttctgtgc aagagtaaag gctttcacat tcccaacaac cctcataatt
     8941 gttttctgtg caacactgtt tttgaaaaca tcccttgtct gccaggtttc tatcacataa
     9001 attattttta cttttgcatc cgtcgcaaga atctttaaga aaacataatt tacaaattaa
     9061 tccgcagtaa gcgatactgt tcgctaagtc ctcggcgtta gttttcggtt tggctttcat
     9121 cttttttcct taacaataaa gattaataat aatccactct atttgactga tctgaaacac
     9181 tgatccagtg tcgatgttac ttctataatt tctaccatta ttctttttgc ctgaataata
     9241 atttctaatg cgacggcaag aatatcgaca ctttagggat tcttgcagtt actgttaatc
     9301 attgttgcgt gctcgccata taatgaagaa ccgaatgtaa gatcataaac cggagacaag
     9361 taccattgtc cggattcatg ttccattata taagaaaaat ttttgaatga tcatcacgtt
     9421 tgtgcacaaa aacattgaaa cacacaaaac tgtacatttt ttcaacctct tcaaaaattc
     9481 cggttggttc aaaagtcagt ttcattaaca gatgataatc aaagttggga tagcggtgac
     9541 tactttcaag aacaccgctt acagatatca tgtaaatctt cttattgtat attcggtcaa
     9601 aacggtttac accaaaataa ccatcgcacc ttttgaaaga aaaaagtctt gtttctgaaa
     9661 acgagataag aaatatttga tacgatgatt cgaatcgctt tacagatcca aacgaccctc
     9721 cccgattttt ttagaaagct cttcctgagt taatcctgat tcttttcgac gtgatcaaat
     9781 gttatccgaa agaattttgt attaactgaa tcactactcg atccacttga tttttttcat
     9841 ctgatcatta ataacagtat atacataatc catgtgttcc tggttttccc agaaagagct
     9901 gtgcttacct tccctgatag tccacagttc actctcgttg ccggcctcct gcattttttt
     9961 aacaaacaaa acagaatcat catagtagac aaggctgtcc cggtttccat gaataatata
    10021 gacaggaaca ctttcaggct taatgtttgt cataggacaa atctgcaaca cctgttcagt
    10081 atcgagagcc actgacatat aaaaattatt atttttaaaa cttacaggcg gggcaaatgc
    10141 ccacacaata ttcggaatcg aactgatttt agggtcttca ccctcttcaa ttatatcggg
    10201 aaacattgaa ttcgcgatta ccaaatggcc gccagccgaa agcccaacag tcgcaatatg
    10261 atcagggtct atatttaact ctttattata ggtacgaagc catctgattg cagagttaac
    10321 atcctttaca cattcgactc cggtgacatt tacataatcc accagacgat attcaaacga
    10381 gacggcaaca aaaccgagag acacaaaatg tttggcatat ggatatccct ctgtcggaga
    10441 tccctgactc caaccacctc catgaaaata ggcagctacc ttatatggct cttctctgtt
    10501 cacatcatat ccttccggat aaaaaatatg aacctcgagc ttgtccattg caaacttgtc
    10561 agagtgaaat tctttataca ctcgtttttc aggcacaccc gaatcagata caattttatt
    10621 aactaacgat gaaggccctt ttgaacagga aataaatggt aaaacagata tcaaaataag
    10681 tattttaaaa aatttcatat agttactctc cgattatgcg ccctatgccg gtcatccagt
    10741 gcctctttga aaatacaaag aggtgctata cctgtcgcaa gaagactctt gctgctcgca
    10801 tgttatatgt catactagtg ttttcaccgt ttaattagta cttttttaca cggtgatatc
    10861 actagataaa ttcttaacaa ttctataaca gtacaattaa tgttcaaatt gttttcggtt
    10921 aataatataa atatgggtta gaactaaagt acgaagcgac ttaattttgg aggtgaatga
    10981 tctgttgctt tcaaaacaat agacattcca agccaaaact cttcacaaaa tgtaactgaa
    11041 gagctttcta tttcatgtgt gaaatatagt tttcttctat atcaaattta aatttctttt
    11101 tgggttttaa tcgaaccgta aaattgagca atatcattgg gcattggaac tcttatctct
    11161 atgacattat tatttagggg atgcgtgaat tttaaacagc tgcaatagag agcctgtcgc
    11221 ctcatatctt ctgaggcaaa accgtacatt tcatcacctg ctattggatg accaatcata
    11281 gccatatgta cccttatctg gtgagtcctt ccggtatgca taatgaattt cagttcactg
    11341 aagtttccat tatttgacaa aacctgatat tgcgttaccg actctttccc cttaccacca
    11401 caaacccttt taatagttcc gtcatctgct cttgagatac aagatgaaat aataccttcc
    11461 gttttttcta atatgccttc tacaatgcca cggtactcct tataaagcat gcctgctttt
    11521 atctgctggc ttattctttc ctgg
//
LOCUS       DAOTGZ010000074        11357 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_3064
            NODE_6788_length_11357_cov_4.653247, whole genome shotgun sequence.
ACCESSION   DAOTGZ010000074 DAOTGZ010000000
VERSION     DAOTGZ010000074.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868088
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 11357)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 11357)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:22:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,785
            CDSs (total)                      :: 2,751
            Genes (coding)                    :: 2,732
            CDSs (with protein)               :: 2,732
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 12 of 19
            Pseudo Genes (internal stop)      :: 5 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11357
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6788_length_11357_cov_4.653247"
                     /isolate="AMR_MDS_3064"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..398)
                     /locus_tag="P5123_06495"
     CDS             complement(<1..398)
                     /locus_tag="P5123_06495"
                     /EC_number="2.7.7.87"
                     /inference="COORDINATES: protein motif:HMM:NF013468.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0003725 - double-stranded RNA binding
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0061710 - L-threonylcarbamoyladenylate
                     synthase [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-threonylcarbamoyladenylate synthase"
                     /protein_id="HRX15944.1"
                     /translation="MSYKTKIFPATESHIKDAATALRAGKLVIIPTETVYGLAANALD
                     AEAASSIFCAKKRPFFDPLICHIADYDMLAILTDNLSPALKKLTKKFWPGPLTIVTVK
                     SEKVPRIITSGLQTVAIRMPAHPVAISIIK"
     gene            complement(388..1704)
                     /locus_tag="P5123_06500"
     CDS             complement(388..1704)
                     /locus_tag="P5123_06500"
                     /inference="COORDINATES: protein motif:HMM:NF012457.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="family 1 glycosylhydrolase"
                     /protein_id="HRX15945.1"
                     /translation="MSIKLPQTFILGSSISAYQTEGANLIDGKKESIWDAFNGSGFTD
                     SLKNGADFYNRFTSDIKTMQKLGISNFRFSIAWSRILPDGTGSLNKKGITFYNRLIDT
                     LLEHNIKPSVTLYSHDLPVRLQKNGGFLNRETCTAFADYTEICANHFGDRIKNWITIE
                     DPLTEIEYGYRTGTMPPGVIAPEKIFVATHNMLIAHAMAYYTLKEANSDNYCGLGVRM
                     DPPLITPTKRDSRIHPNAEAYLVKLLIDPVIKGYYPAVYESEIYSQNRLNLRPKDTML
                     IHNSYDYIALAHSGYTHVKSKIVNVNINDPLSFSITQYPALENRYSKTIGYDPSAIQG
                     ILKTLQTEYGNPKILFANVGYPSDNHDAQNRINFYHAILSAIADAIANGGKIEGFFTK
                     SFIDGFELINPTRQRSGLVKVNFTTFEREVTDTARWFSKVCKNNEL"
     gene            complement(1701..2501)
                     /locus_tag="P5123_06505"
     CDS             complement(1701..2501)
                     /locus_tag="P5123_06505"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HRX15946.1"
                     /translation="MTIIDLKNISCTPDPYLPPVLKNINWTVNQNERWILFGANGSGK
                     SMLLQIITGYRRNSHGSIDRFGEGETGTDIRQVRKKIGYVASGLRSMVFPNETIIDLV
                     ISGHFATIGLYDTPHNEIREKAEELLVHTGLMDRKNDLFSHLSDGEKQKIMMARALIN
                     SPELLILDEPASALDIAAREDLLITLHKMLEGHKGALIYVTHHIEEITPLFSHCLMLK
                     KGGVFKIGSIDDTINGNVLSSLFGIPLSVTKNNGRFVPLLSAKGATQS"
     gene            complement(2507..3601)
                     /locus_tag="P5123_06510"
     CDS             complement(2507..3601)
                     /locus_tag="P5123_06510"
                     /inference="COORDINATES: protein motif:HMM:NF013528.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DHH family phosphoesterase"
                     /protein_id="HRX15947.1"
                     /translation="MNKPNYKEIKTSLLNIFSKYNNLLIVIQGSPDPDAIASSFFLMK
                     MAQTCDCSCTVIAGKTISLPQNRELISKLDIILNHNYPENFDEYDGYCVVDYQNPAVE
                     NIPSQVPCVLHIDHHTRAESSINPEYSLYPEDVNSVSTVLAGVLLQQNDLFDEKIRQQ
                     IATALAYGIKTDTDNLTIASTTDKEAFSEMEKIADKEILDLLDNMPYSEETLTLISKA
                     MLNSTNYKSWLISGLGFIPENLRDSLAITADYMLEQENVTTVIAFALITSENSITLDA
                     SFRTKNSELDLNGIIKSITPDGGGRSFKGAFQLDLSYFAGYPEKHNLWSIVNESTLIN
                     IKKARDNISFYEVRTFFTGLKKQLSDIFHR"
     gene            3866..4501
                     /locus_tag="P5123_06515"
     CDS             3866..4501
                     /locus_tag="P5123_06515"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRX15948.1"
                     /translation="MSNESKQSVFQTYEHERAFKNIQVLLSDEYSIEEAAERIIEGTF
                     MGMDIEKKDSLDRFIDYVVFKARTGVPYIENMAYPSMRFENREMENKVKGLINEHLMP
                     EIVIKVLKFFARNIQNSDNNLHLAALIDNELIINSVFETFKTFREDVFVTDRDKKTLN
                     VKRIQQFSAHTDPRFSNPLDAACRFKYILEYFALRKNVSHIYTKEDILMAV"
     gene            complement(4517..5422)
                     /locus_tag="P5123_06520"
     CDS             complement(4517..5422)
                     /locus_tag="P5123_06520"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HRX15949.1"
                     /translation="MEIFLVSFSCLFQILTALVALYTIRITGTRLSWIMISLAAILMG
                     ARRVLSLIGIMENRMEENICYHSAGLAISILLFFGTLGIRSIFADWNITKKKNEELLK
                     QKKILLKEVHHRIKNNITSISQFLKIQADKEQLKESKMVLLEATSRVDGMKLLYNKLL
                     ISDNYGTVSTNNYITELLDSIISLFPDKEKICLNAEIDNPPLDIQTLITLGSIINEIV
                     TNSVKYGFDGRQTGIIDVSLKDNDSEITLIVSDNGKGFDSSFPDKEKGLGLTLVTMLV
                     QQLKGDFMINSENGTKSVIRFTYKR"
     gene            5641..6141
                     /locus_tag="P5123_06525"
     CDS             5641..6141
                     /locus_tag="P5123_06525"
                     /inference="COORDINATES: protein motif:HMM:NF016213.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF456 domain-containing protein"
                     /protein_id="HRX15950.1"
                     /translation="MPYIHEIVLVIAIIFAILGVAGCVIPAIPGPFVSFGALLMLNWT
                     KFADVSIWAIIVFGFFAVLVTVLDYVLPPMGSLKFGGTKRGSVGATIGLFVGIFFFPP
                     LGIFIGAFVGAFIAEKTGGMETDPSLKAAFGAFLGVITGVVVKFLLSISIFVFMTVYT
                     VSYFVS"
     gene            complement(6168..6752)
                     /locus_tag="P5123_06530"
     CDS             complement(6168..6752)
                     /locus_tag="P5123_06530"
                     /EC_number="3.4.21.105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006101136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rhomboid family intramembrane serine protease"
                     /protein_id="HRX15951.1"
                     /translation="MKKNRFSQKIKTLLTLIAIMWIVLAVNFFIFHNSLLHYGIAPRS
                     VSGLIGIFFAPFLHANKVHLTSNTVPFLILGYLSMRNGTGEFYSVFIVSMITSGLGTW
                     IIGSSGTVHIGASGIVFGLFGYLLLKGYFQRKPISIITSILVAFLYGGMIFGILPKYP
                     GVSWQCHLFGFLGGILAAKTMSRRITINTNNINY"
     gene            6870..8087
                     /locus_tag="P5123_06535"
     CDS             6870..8087
                     /locus_tag="P5123_06535"
                     /inference="COORDINATES: protein motif:HMM:NF014164.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase"
                     /protein_id="HRX15952.1"
                     /translation="MSSYEERYAEVIIRTGVNLYPGQCLNVTTGYHNYDFATVIAEVA
                     YKAGARYVNIDVVSNKERKIRINNSESCYLEYVPEFSVSRYHEMCADDWAFVRIDDTE
                     ELSVLEGVDPEKLGIIEKAMRKSKSVFYKNLLKDKICWCVVCAPGPKWADYLLGNEST
                     DGLWQLLSPILRLDHDDAVKAWSEHGERLLARRRILDEMNIKTLHFKNADTDLKIGLT
                     STSRWHGGPSYTLDKRLFIANIPTEEVFTTPDFRKTEGYVKVTRPLKVLETMVEGASF
                     EFKDGKVVNFSAQKNEAVLRKYLAIDEGASFVGEVALVGCDSPLYTSGKVFGSILYDE
                     NAASHIALGSGYPSCLSNMNSLHGDDDLKSAGCNVSLVHTDFMIGSESMRVTAVCEDG
                     KEVVVMENGLFVI"
     gene            8096..8461
                     /locus_tag="P5123_06540"
     CDS             8096..8461
                     /locus_tag="P5123_06540"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRX15953.1"
                     /translation="MIFKKKKNTKRIRQCRERLENCAAVLETSKKRISEALKAFETDE
                     NCEVIAPIKTLVDNLVRLEDYVEAVLKEIKGIENNKDIEKIEKEKIDQIVNQADEMSV
                     TALQAVSNSLKAFNPDNKD"
     gene            complement(8482..9576)
                     /locus_tag="P5123_06545"
     CDS             complement(8482..9576)
                     /locus_tag="P5123_06545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015616790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toxic anion resistance protein"
                     /protein_id="HRX15954.1"
                     /translation="MADGMLQKREPRELIDRSKFLPEDLAKIEEIKKGFNIDNSDEVI
                     GYGVGAQREISDFSDTILDQVRSKDSGFVGEVLTDLMVTVKDIDVDSLSTKGSFMSSL
                     PIIGDMMDKAKRFIARYDKLSVQIEKIIEELEKARMNLLKDITLLDNLYEKNLSYLKN
                     LDLYIAAGEDKIEELNTTVLPQYKQKADQTNDPADAQRYHDLAQLINRFEKKLHDLKL
                     SRMISIQTAPQVRLIQNGDQVLVEKIQSSILNTIPLWKNQIVIAITLFRQDKALKLQK
                     EISETTNNLLKKNSEMLKDGTIEVAKESERGIVEIETLKKVNNDLLSTIEETIKIQQE
                     GKTKRKNAEVELIKLESELKTKLKEVKSQS"
     gene            complement(9597..10217)
                     /locus_tag="P5123_06550"
     CDS             complement(9597..10217)
                     /locus_tag="P5123_06550"
                     /inference="COORDINATES: protein motif:HMM:NF021617.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-bromo-4-chloroindolyl phosphate hydrolysis
                     family protein"
                     /protein_id="HRX15955.1"
                     /translation="MIGVILVFIIIISVVLFIIYMIHLLFIPIIIALAILIFRKARKK
                     DEPQTSMYLNGVENAEVRAVLKKGFDKQQTLKKLRVKVSKREIREKVNRIVDVVDRIF
                     ENLKKDPKDIKAARQFLNYYLDSTITILEKYVQLSNHGAAGSELKTTLEKTESMLDNI
                     EHSFEVQLGKLLSNDVMDLDTEIKLLEKTFKMEGLDIKRPKETIEK"
     gene            complement(10282..10896)
                     /locus_tag="P5123_06555"
                     /pseudo
     CDS             complement(10282..10896)
                     /locus_tag="P5123_06555"
                     /inference="COORDINATES: protein motif:HMM:NF003994.0"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="redox-sensing transcriptional repressor Rex"
BASE COUNT         3201 a         1977 c         2413 g         3766 t
ORIGIN      
        1 tcctttataa tagaaatggc aacaggatgg gcaggcattc ttattgctac ggtttgaaga
       61 ccggacgtta taatccgggg aaccttttca gatttgaccg taactattgt tagggggcct
      121 ggccagaatt ttttggttaa tttctttaat gccggcgaaa gattatcagt taaaatcgca
      181 agcatatcat aatctgcgat atggcagatt agcgggtcaa aaaatggacg tttttttgca
      241 cagaatatgg aggaggctgc ttcggcatca agcgcgttcg cggcaagccc atagaccgtc
      301 tcggttggaa taataacaag ttttcccgca cgaagagcgg ttgcagcgtc cttgatatgg
      361 ctttctgttg ctggaaaaat ttttgtctta taactcattg tttttgcaca ccttggaaaa
      421 ccacctggct gtatcggtaa cttcacgttc gaatgttgta aagtttactt tgacgagccc
      481 ggatctttgt cgagttggat taataagctc aaagccgtca ataaaacttt ttgtaaaaaa
      541 cccttcaatt ttaccaccgt tagcaattgc gtctgctatg gcagatagta tggcgtggta
      601 aaaattaatg cggttttggg catcgtgatt gtctgaagga tatccaacat ttgcgaaaag
      661 tatcttcgga ttaccatact cggtttgaag tgttttcagg attccttgaa tagcggatgg
      721 gtcataaccg attgtttttg aatatctgtt ttcgagtgcg ggatattgag taatgctaaa
      781 agataaggga tcgttaatat tcacgttcac aattttgctt tttacatgag tgtatccgga
      841 atgcgcgaga gcaatatagt cgtacgagtt gtgtatcagc attgtatctt taggccgtag
      901 atttaatctg ttttgactgt aaatctctga ttcgtaaacc gctgggtaat agccttttat
      961 tacaggatct attaaaagtt ttacaaggta agcctcggcg tttggatgta ttcttgaatc
     1021 tctttttgtt ggggtaatca agggaggatc cattcggaca cctaatccac aatagttgtc
     1081 ggaatttgcc tcttttagag tatagtaggc cattgcgtga gctataagca tgttgtgcgt
     1141 ggcgacaaaa attttttcgg gtgcgataac ccccggaggc attgtgccag tacggtatcc
     1201 gtactcgatt tccgttaaag gatcttctat cgtaatccag ttttttattc tatcaccaaa
     1261 atggttggcg caaatctcgg tgtaatcggc aaaggcagtg caggtctccc tgtttagaaa
     1321 tccgccattt ttttgaagtc ttaccggtaa atcatggctg tatagcgtta cggacggttt
     1381 tatattatgc tcaagaagag tgtctatcag acggttgtaa aaagtaatgc cctttttgtt
     1441 aaggctcccg gtgccatcag gaagaatacg gctccaggcg atagaaaaac gaaagtttga
     1501 gatgccaagt ttttgcattg tttttatatc agaggtaaat ctgttataaa aatcagctcc
     1561 gtttttaagg ctgtcagtaa aacctgatcc gttaaacgcg tcccaaattg actctttttt
     1621 gccgtcaatt aggttagctc cctctgtctg gtaggccgag attgaactgc caagtataaa
     1681 tgtttgtgga agttttatac tcatgattga gtagcgcctt tggcagataa tagaggtaca
     1741 aagcgaccgt tattctttgt taccgataag ggaatcccaa aaagcgaact taatacattg
     1801 ccgttgatag tgtcatcaat cgaacctatt ttgaatacac cacctttctt tagcataagg
     1861 cagtgactaa aaagtggtgt gatctcttcg atatggtggg ttacatagat aagcgcgcct
     1921 ttatggcctt cgagcatttt atgcaaggta atcagaaggt cttcacgagc agcaatatca
     1981 agagcggagg ctggttcgtc aagaataaga agttccggac tgttaatgag agcccgagcc
     2041 atcatgattt tctgtttctc tccgtccgaa agatgcgaaa aaagatcatt tttacgatcc
     2101 ataagaccgg tatgtacgag tagctcttcg gctttttcac gtatttcgtt gtgaggagtg
     2161 tcataaagac cgatggtcgc gaaatggcca cttataacaa gatcgataat tgtttcgtta
     2221 gggaaaacca tcgagcgtaa tcccgaagcg acgtatccga tttttttacg tacctgcctt
     2281 atatcggttc cggtttcacc ttctccgaaa cggtcaatgg agccatgtga gttacggcgg
     2341 taacctgtaa ttatttgtag cagcatgctt ttgcccgaac cgtttgctcc aaagagaatc
     2401 catctttcgt tctggttaac tgtccagttt atgtttttta gaacgggcgg taggtaagga
     2461 tcaggagtac aactgatatt ttttagatcg ataattgtca tttgtgctac ctgtgaaaaa
     2521 tatcggataa ctgttttttt aatccggtaa agaaggtacg tacttcgtaa aaagaaatat
     2581 tgtcacgagc ttttttaata ttaatcagtg tagattcgtt tactattgac cacaggttat
     2641 gcttttccgg atatccggcg aaatagctta gatcaagctg aaatgctcct ttaaaacttc
     2701 ttccacctcc atccggagtt atggatttga tgattccgtt aagatccagt tcactattct
     2761 tggttcgaaa agatgcatcc agtgttattg aattttcaga agttatcagc gcaaacgcga
     2821 taactgtagt tacgttttcc tgttcaagca tatagtcggc agtaattgcg agtgaatccc
     2881 ttaaattttc aggaataaaa ccaagacccg agattagcca gctcttatag ttggtgctgt
     2941 taagcatggc cttgctgatt agagtaagtg tctcttcgga atatggcatg ttatccagta
     3001 aatccaggat ttctttgtca gcgatttttt ccatctcgct gaaagcttcc ttgtcggttg
     3061 ttgaagcgat ggtcagatta tcggtgtcgg tttttattcc gtacgccagt gctgtggcta
     3121 tttgctgtct gattttttcg tcaaagaggt cgttttgttg tagcaatacg cctgcaagta
     3181 ccgtagaaac ggagttaaca tcttcgggat acaggctgta ttcgggattt atactgcttt
     3241 cggcacgtgt atggtggtca atatgcaaaa cacaggggac ttgtgacgga atgttctcta
     3301 ctgccggatt ttggtagtca acaacacagt aaccgtcata ctcatcgaaa ttttcaggat
     3361 agttatgatt aagaataata tccagtttgc tgattagctc ccggttttgt ggcaaagata
     3421 ttgttttgcc agcaataacg gtacaactgc aatcgcaggt ttgagccatt ttcatcagga
     3481 aaaaagatga agcaatagca tcgggatcgg gtgacccctg gataacaatc aatagattat
     3541 tatatttact gaaaatattc agtaaacttg tttttatctc tttgtagttc ggtttattca
     3601 ttgtttattt cgtctctttt ttatctgttt accataaaac tggtttatag ctcagttatc
     3661 aataaataaa aataaagaaa ttctttctgt tgaggaactt ctgaaaatta caatttttag
     3721 agactgtttt cgtttaaaag ccgattttat aggaatttgt cgtaagttgc tgctcacagt
     3781 gttaattttt agaaattgtc ggttttgttt tttttgattt gggtcgattt aatagtaaat
     3841 gatcgctttg ggagaaactg taaaaatgag taacgagagc aaacagagtg tttttcagac
     3901 atatgagcat gagcgcgcgt tcaaaaatat tcaggtgctc ttatcggatg aatacagcat
     3961 tgaagaggcc gcggaacgta ttatcgaagg aacctttatg gggatggata ttgaaaaaaa
     4021 ggattccctt gacagattca tagattatgt cgtattcaag gctcgtacag gagttcccta
     4081 tatcgaaaac atggcttacc cttcgatgcg gtttgaaaat cgtgaaatgg aaaacaaggt
     4141 aaaaggtctg ataaacgaac atcttatgcc ggagattgta atcaaggttt tgaagttttt
     4201 tgcgcgaaat atccaaaatt ctgataacaa tcttcatctt gcggcgctta tagataacga
     4261 attgataata aactcggttt ttgaaacctt taaaacattc agggaagatg tatttgtaac
     4321 agatcgagat aaaaagacat taaacgtaaa acgtatacag cagttttcgg ctcatactga
     4381 tccacgtttt tctaaccctc ttgatgcagc atgtcgtttc aagtacattc ttgagtattt
     4441 tgctttgcgt aagaatgtat cacatattta tacgaaagaa gatatactta tggcggttta
     4501 accgcattat aggtagttac cgtttgtaag taaacctgat aaccgatttt gtaccgtttt
     4561 cactgtttat cataaaatca cctttaagtt gctgaacaag catggttact agagtgagtc
     4621 ccaaaccttt ctccttatcg ggaaaggagc tgtcaaagcc tttgccgtta tctgaaacaa
     4681 tcagggttat ttcgctgtcg ttatctttta gtgaaacatc gattatacct gtttggcgtc
     4741 cgtcaaaacc gtacttcacc gaattagtaa caatctcgtt aatgattgaa ccaagagtga
     4801 tcagagtctg aatgtcaagt ggaggattat ctatttcggc gtttaagcag attttctctt
     4861 tatcaggaaa aagagatatt attgaatcca gaagttctgt gatatagttg tttgtagaaa
     4921 ccgtgccgta attgtcactg ataagaagtt tgttatagag gagtttcata ccgtcaacgc
     4981 gtgaagttgc ttcaagtaga accatttttg actcttttaa ctgttcttta tcggcctgta
     5041 tcttcagaaa ttgtgaaatc gaagtaatat tattttttat tctgtgatgc acctctttta
     5101 aaaggatctt tttctgttta agaagctcct catttttctt tttagtgata ttccagtccg
     5161 cgaaaatgga tctgatgccc aaggtgccga agaatagcag tattgaaatg gcgagcccag
     5221 cagaatgata gcatatgttc tcttccatac ggttttccat tataccaatt agtgaaagaa
     5281 ctcttctggc acccattagg atcgcagcca aacttatcat tatccatgac aaccgtgtac
     5341 cggttattcg gattgtgtag agagctacaa gggcagtcag aatctgaaat aggcaagaga
     5401 agctaaccag aaaaatttcc atttttatgt cctaatagtg ttctgatgat atcaaacagt
     5461 aaagtagtaa ctactttcag gtatatcaac aaataatccg aatttttatt acatggctgg
     5521 gattgtaaac tcttactgtt ttattgtttg caaaaaatga tttttcataa aataaataat
     5581 ctttgacatg gaagtttata attattcttt taatcaaatt ataagggaaa ggcggatact
     5641 atgccatata ttcatgagat tgtattggtt attgcgataa tattcgcgat tcttggcgtt
     5701 gccggttgcg ttataccggc cattcctggt ccttttgtaa gttttggcgc tttattgatg
     5761 cttaactgga ctaaatttgc ggatgtttcg atctgggcca taattgtctt tggttttttt
     5821 gccgttctag ttacggttct ggactatgtt ttgccgccga tgggctcttt aaaattcgga
     5881 ggaaccaaga gagggtctgt aggtgcaacc attgggcttt tcgtaggtat cttttttttt
     5941 ccaccccttg gtatttttat cggggctttc gtgggagcat ttattgcgga aaaaactggt
     6001 ggtatggaga ccgatccttc gttaaaagct gcttttggag cttttttggg agttataacc
     6061 ggcgttgtag tgaagtttct gctttcaatt tctatatttg tatttatgac cgtttataca
     6121 gtaagctatt tcgtgagctg atatcagata tcgtgatttt taacagatca atagttgata
     6181 ttatttgtgt ttatcgttat tctgcgactc atcgtttttg ccgctaaaat tccacctaga
     6241 aaaccgaaca ggtgacactg ccatgagacg ccagggtatt ttggcagaat gccgaatatc
     6301 atgccgccgt ataaaaatgc cactaaaatc gatgttataa tggaaatcgg ttttcgttga
     6361 aagtagccct ttaataagag atatccgaac agaccgaaga caataccact ggctccaata
     6421 tgtactgtgc cagatgaacc gattatccat gtgcccaatc cggatgttat catggacacg
     6481 ataaagacag aatagaactc tcccgtaccg ttgcgcattg aaagatatcc aagtataaga
     6541 aatggtactg tgtttgaagt gagatgaacc ttgttagcat gcagaaaagg agcaaaaaaa
     6601 atacctatca ggccggaaac agagcgtgga gctattccgt aatgcagtag agagttgtga
     6661 aaaataaaaa aatttacagc tagcacaatc cacattattg ctattagtgt taacagggtc
     6721 tttatctttt gtgaaaatcg gttctttttc atggctatgt tccttatatt atagttgaaa
     6781 atgaatactg ttttacaaaa aaacaagaaa aatatataag ggtattaaaa attgttaatg
     6841 ttggtacatt atataagcgg agagcagtta tgagcagtta tgaagagcgg tacgccgaag
     6901 taatcatcag aaccggagtg aatctttatc ccgggcaatg tcttaatgtg acaactggtt
     6961 atcacaatta tgatttcgca actgtaatag ccgaggttgc ttataaagcc ggagctcgtt
     7021 atgtaaatat cgatgttgtt tcaaataaag agcggaaaat acgtattaat aattcggaat
     7081 cgtgttatct ggaatatgtg ccggaatttt cggtgtcccg ttatcatgag atgtgcgcgg
     7141 atgactgggc ctttgtacga atcgatgata ctgaagagct gtcagtgctc gaaggagttg
     7201 atcccgaaaa actgggaatt attgaaaagg ctatgcgtaa atctaaatcg gttttctata
     7261 aaaatctgct aaaagacaag atctgttggt gcgttgtgtg tgctcctggc ccaaaatggg
     7321 ccgattacct tcttggaaac gaatctactg atggtctatg gcaactcttg tcgcctattt
     7381 tacgtctgga tcatgatgat gctgttaagg cgtggagtga gcatggcgag cgtttacttg
     7441 ctaggcgaag aattcttgat gaaatgaata taaaaacatt gcactttaaa aacgctgata
     7501 cagatcttaa aattggtctc acctcaactt caagatggca tggaggtccc tcgtacacgc
     7561 tggacaaacg tctttttatt gcaaatatac cgacagaaga ggtttttacc acgccggatt
     7621 ttcgcaaaac cgaaggatat gttaaggtaa ctcgaccatt gaaggttctt gaaacgatgg
     7681 tcgaaggggc gtcttttgaa tttaaagatg gcaaggttgt taacttttcg gcgcaaaaaa
     7741 acgaagcggt cttgagaaag tatctggcta ttgatgaggg agcgtctttt gtaggtgaag
     7801 tggcactagt tggatgcgat tctccgcttt atacaagtgg aaaagtattt ggttctatat
     7861 tgtatgatga aaacgctgct tcgcatattg cccttggaag tggttatccg tcatgcttgt
     7921 ctaatatgaa cagcctgcat ggtgatgatg atttgaagag tgccggctgt aatgtttcac
     7981 ttgtacatac agattttatg attggttccg aaagtatgcg tgtaacagcc gtatgtgaag
     8041 acggtaagga agttgttgtt atggaaaatg gattatttgt aatataaggg gtgttatgat
     8101 ttttaaaaag aagaaaaaca cgaagcgcat acgtcaatgc cgtgaacgtc ttgaaaattg
     8161 cgcggccgtt cttgaaacaa gtaaaaagcg tatttcggag gcattaaagg ctttcgaaac
     8221 cgatgaaaac tgtgaggtaa tagcgccaat aaaaacactt gtggacaacc tagtgcgtct
     8281 ggaagattat gtagaggctg ttttaaaaga gattaagggt attgaaaaca ataaggatat
     8341 cgaaaaaatc gagaaagaaa aaatagacca gattgtaaat caagccgacg aaatgagcgt
     8401 aacggctctg caggcggtta gtaattccct aaaagccttc aatcccgata ataaggatta
     8461 aagactttta agtgatagat attatgattg gctcttgact tcttttaact tggtttttag
     8521 ttcgctttca agttttatga gttcgacttc tgcgttttta cgttttgttt tcccttcctg
     8581 ttgtatcttg atagtttctt ctattgtgga gagcaggtca ttgttgactt ttttcaatgt
     8641 ctctatttcg actattcccc gttcgctctc tttcgcaact tctattgtgc cgtctttaag
     8701 catctctgaa ttttttttca gaagattatt ggtcgtttcg cttatctctt tctgtagttt
     8761 taacgccttg tcctgtctga aaagtgttat agcgataaca atctgatttt tccatagagg
     8821 tattgtgtta aggatggagc tttgtatctt ctcgacaaga acctggtcgc cgttttgtat
     8881 aagccttacc tgtggtgctg tctgtatcga tatcattctt gatagtttaa gatcgtgaag
     8941 ttttttttcg aaacggttaa ttagctgggc aaggtcgtga taacgttgtg cgtcggcagg
     9001 atcgtttgtc tgatcagctt tttgcttgta ttgaggcaaa acagtggtat tcagctcttc
     9061 tattttatct tcgcctgccg ctatataaag gtcaagattc ttaaggtaag agaggttttt
     9121 ttcgtacaga ttatcaagca gagtgatatc tttaagaagg ttcatcctgg ctttttcgag
     9181 ctcttcaata attttttcta tttgaacaga aagtttatcg tatctggcaa tgaatctttt
     9241 tgccttatcc atcatgtccc cgattatggg aagagacgac ataaagctgc cttttgttga
     9301 gagggagtct acatcgatat cctttactgt caccataagg tcggtcagaa cctctcctac
     9361 aaaacctgaa tcttttgaac gaacctgatc gagtatcgta tcggagaagt cggatatctc
     9421 tctttgggca ccaactccgt aaccgattac ctcatcggag ttgtcgatat taaaaccttt
     9481 tttaatctct tctattttag ccaaatcttc gggtaagaat ttcgagcgat caataagctc
     9541 tcttggttct cttttttgaa gcattccgtc tgccatagta ttctccttta gtatacttat
     9601 ttctctattg tttctttggg gcgcttaata tcaagccctt ccattttaaa ggttttttcc
     9661 aatagtttaa tctctgtgtc aagatccatt acgtcgttag agagaagttt gccaagctgt
     9721 acttcaaaag agtgttcaat gttatcgagc atgctctcgg tcttttccag agtggtcttt
     9781 agttcggagc ctgcggcacc gtgattagaa agttgaacgt atttttcgag tatggttatt
     9841 gtagagtcaa gataatagtt taaaaactgt ctggcggctt ttatatcttt gggatctttt
     9901 tttaggttct caaaaatacg gtcgactaca tcgacaattc tgttaacctt ttcgcgtatt
     9961 tcacgtttag atacctttac acgtagtttt ttcagggttt gctgtttatc aaagcctttt
    10021 ttcagtacag cacgaacttc cgcgttttct acgccgttaa gatacatact tgtttggggc
    10081 tcgtcttttt ttcgtgcctt gcgaaaaata agtatagcaa gggcaataat aatgggaata
    10141 aaaagaagat gaatcatata aataataaat aagactacag atattattat tataaaaaca
    10201 agaattacac caatcatgaa ctactccgca acactatgat tattccataa tatggtacaa
    10261 aatatgaaag tcaagttact ttcaacgagc attaacaaag tagaagaggc tttcaagctc
    10321 tacatcgaga ttgatatatt taattattac atcaccggta tcttttaagg taaccggggc
    10381 aaagttcata acggcgagaa ttccgctctc tttaagctga tcgaaaactt cctgagcgaa
    10441 acgtgcaggc attgtgagta ttgccacctt tacctcatta tcattgatgt actttttcat
    10501 gtcttcaaga ggtagtattg gaacaggcct ggaaagtgaa attttcttgg gatttgagtc
    10561 aaaaccggca catatttcga tattttcttc tttaaagttc tggtaatcca ttagtgcttt
    10621 ggctattttt ccacagccga caataatggc gctgttctta tcttttttaa tcagaatttc
    10681 attgatgcga tcgatcaatt cgttgatatc gtaacccgat tttttattac ctgttatttt
    10741 tagaagcgag aagtcttttc ttacctgtgt cgatttttca ccagtaagct cggcaagaat
    10801 attggaaaat actctctctt cgcccgagtt cagaattctt tttaatacat ttttgtaccg
    10861 tataagacgg attattcttt ttcgattaat catttgattc gccgcttgct ttttttgtaa
    10921 ggaaacttca aaaaattaag tataaaaaag gcaattttaa acagtgtttt gctttgcgca
    10981 aatatgtcgt tagtttactt attggatact ttttgtgaaa acgtaccgaa taatgatctt
    11041 tattgcctgt cgggaaaaga agatagaaga ttccatagaa taattccttt cgattatggc
    11101 ttatttgtca agtataaggg gtaaaatgtg ataaaaatca caaaaagaga ggatatgcta
    11161 tcgcagttct tttttctttg acagatgtga gttgatatga ttgagtgaaa acacgttttg
    11221 gaggttactg tgagcagaga tagttttaga caggcgatat ttaatgtggt tgaacatata
    11281 acaaattatg agttgtctgc tacggcacaa aaattgattc ttcactattt taacggttcc
    11341 aaacagaaga gctctta
//
LOCUS       DAOTGZ010000075        11334 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_3064
            NODE_6814_length_11334_cov_4.783137, whole genome shotgun sequence.
ACCESSION   DAOTGZ010000075 DAOTGZ010000000
VERSION     DAOTGZ010000075.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868088
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 11334)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 11334)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:22:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,785
            CDSs (total)                      :: 2,751
            Genes (coding)                    :: 2,732
            CDSs (with protein)               :: 2,732
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 12 of 19
            Pseudo Genes (internal stop)      :: 5 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11334
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6814_length_11334_cov_4.783137"
                     /isolate="AMR_MDS_3064"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(206..1174)
                     /locus_tag="P5123_06560"
     CDS             complement(206..1174)
                     /locus_tag="P5123_06560"
                     /inference="COORDINATES: protein motif:HMM:NF040385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6353 family protein"
                     /protein_id="HRX15956.1"
                     /translation="MNSLALVKSLIPTKLGRVGLILRKNSPEIMIGLGVVGLSYSVWK
                     IAKAEPKARKIKEEYYSELAEIEEVKDHLISNNKDYTDNDYIKDRGLVAFQAGVDLVK
                     LYGPPVVLGAVSVACIFGSHIIMRNRNIALMAAYTAIDDAFKRYRKRVVEDYGEEVDR
                     RFRLGMRQQEIIVEEEDEKGKKKKVKKNVDVVDPNGISVYARFFGKGYSSEWSDTPNY
                     NLSYIKGQQSFFNQLLHSRGHVFLNEVYDALGFKRSTEGALVGWVLGGGGDDFIDFSI
                     YNCSTPEYRNEYSCDTIAEERQDFVNGYRDSILLDFNVDGLIYDKI"
     assembly_gap    1211..1310
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(1394..1615)
                     /locus_tag="P5123_06565"
     CDS             complement(1394..1615)
                     /locus_tag="P5123_06565"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRX15957.1"
                     /translation="MNEVLDLLADGKHVIIVMFKKEINAFIVTVTDNKGNTYSKEVER
                     DEAESVKYDALTAAIRQGIYVLKLREDLK"
     gene            complement(1629..2297)
                     /locus_tag="P5123_06570"
     CDS             complement(1629..2297)
                     /locus_tag="P5123_06570"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRX15958.1"
                     /translation="MDEEKREPLKVDNGSRGVRILNSDYPSNSRRVVTEKKEEKKVKK
                     IISGKVILKKKPFLQRVKDSLFGDGLAIAGSYVLNEILVPAAKSTISDMINGATDMML
                     FNDDRHRPRRERGGRTIYTPYDKRYDRGRDREERQYSRVRRGFEDIIFSSRRDAEEIR
                     DHLVDMITEYGAVSIADLYDAVEIDSTNFTDDKYGWTSLRNAVITRDRDGYVLELPKP
                     KLID"
     gene            complement(2578..2847)
                     /locus_tag="P5123_06575"
     CDS             complement(2578..2847)
                     /locus_tag="P5123_06575"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRX15959.1"
                     /translation="MTNKKLIIADMVVGITAGIGTLAIVEQVVSSVVPPQIKIGSKIF
                     IGVAKIVISCMAADAVVGHVSKTFRAIDHVMSELEVETTEKPNEK"
     gene            complement(3030..3374)
                     /locus_tag="P5123_06580"
     CDS             complement(3030..3374)
                     /locus_tag="P5123_06580"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRX15960.1"
                     /translation="MVVNSLESLGTPAYLYRISKRRQNLETLKGYARIDRYGKGEFIV
                     RVDRPSGPRYRRLVCSTEESKVHHSVVWLTNDDSKLARIILIDHWGREIEKINDELAK
                     YINTIKILLEGL"
     gene            complement(3455..3634)
                     /locus_tag="P5123_06585"
     CDS             complement(3455..3634)
                     /locus_tag="P5123_06585"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRX15961.1"
                     /translation="MGNVLNFVKSNWKIGIAILGGIALVGLAKAVANKCGDDDEYDDE
                     EIDYIETTIVETTEE"
     gene            complement(3698..4474)
                     /locus_tag="P5123_06590"
     CDS             complement(3698..4474)
                     /locus_tag="P5123_06590"
                     /inference="COORDINATES: protein motif:HMM:NF040385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6353 family protein"
                     /protein_id="HRX15962.1"
                     /translation="MPKPLIKVIGRFLVKNSPTIMATLGGAGVVSTAILGIKATPKAL
                     LLIEEEVRRKYSGYNYRVLTSKPLLSKKEVIKITWHCYIPTMIMGGVTLACIFYGNSL
                     NLKRNAALASMYTLAETTLKEYQNKVVEVVGEKKAKKIEDEVYEEEIKKHPVDVSEIT
                     ITGRGDTLCYERTSGRYFKSDIENIRRVRNNLNELLLSDGFVKLNELYYELGLPETSI
                     GEYWGWDISKGQIDIKFSSQLTQHGTPCLVIDSEPTYFSD"
     gene            complement(4732..4884)
                     /locus_tag="P5123_06595"
     CDS             complement(4732..4884)
                     /locus_tag="P5123_06595"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRX15963.1"
                     /translation="MGNYAKIVFSNRELLGMAISVIGCVIIGNGITELIKAGNSKIET
                     FLKKGA"
     gene            complement(4971..5192)
                     /locus_tag="P5123_06600"
     CDS             complement(4971..5192)
                     /locus_tag="P5123_06600"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRX15964.1"
                     /translation="MGGKIMFHALALGYLGFVGFCYSKSQYYRGRIDGIKDVTDTLTE
                     MVKAEFGIDTEELKKEKDLRNKKLTLVKK"
     gene            complement(5274..5582)
                     /locus_tag="P5123_06605"
     CDS             complement(5274..5582)
                     /locus_tag="P5123_06605"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRX15965.1"
                     /translation="MDIKKFIKNHKKEIIIGTVVTISVVGLIVKIKHGKGLNLVNSSG
                     LNAITWDANQPETFMTLERVKELLDLNANNDSMFAIFKEFAEADKYVCLILNDSVALV
                     "
     gene            complement(5669..5893)
                     /locus_tag="P5123_06610"
     CDS             complement(5669..5893)
                     /locus_tag="P5123_06610"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRX15966.1"
                     /translation="MNKLEKINFIGHVFMNGAKIQMSQEWVIVLAAIVGLSQGSFKQG
                     IIGGLAIFGTCGAVGSVLGIMDNWDKIKNL"
     gene            complement(5886..6227)
                     /locus_tag="P5123_06615"
     CDS             complement(5886..6227)
                     /locus_tag="P5123_06615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009331358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5664 domain-containing protein"
                     /protein_id="HRX15967.1"
                     /translation="MIKDSGKRHEYETGAVRDLHKGKGRYDLIPWDAIHELALHCEEG
                     ALKYGERNCEKGIPVHSLIDSAVRHLSCYLRGMKDEPHLRAAMWNIAFAIWTEKNHPE
                     QQDIPMRYRNE"
     gene            complement(6214..6423)
                     /locus_tag="P5123_06620"
     CDS             complement(6214..6423)
                     /locus_tag="P5123_06620"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRX15968.1"
                     /translation="MEEKLRLLAKESAELTYLSLLFAELLRNQHMFYTPDFTSLDELI
                     DEHLVSTLNLFYQILPKMEECNDKR"
     gene            complement(6467..6619)
                     /locus_tag="P5123_06625"
     CDS             complement(6467..6619)
                     /locus_tag="P5123_06625"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRX15969.1"
                     /translation="MNNEIKNRKVKPDTIAIVMTNLIGIGMILGYERVNIITSKALGF
                     VLKGRV"
     gene            complement(6983..7174)
                     /locus_tag="P5123_06630"
     CDS             complement(6983..7174)
                     /locus_tag="P5123_06630"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRX15970.1"
                     /translation="MQRSFIERLKSRKFLAAFFTALFIICNEGLGLGVDQQAYMWITG
                     VVTAFILGESYIDAQAIGK"
     gene            complement(7174..7875)
                     /locus_tag="P5123_06635"
     CDS             complement(7174..7875)
                     /locus_tag="P5123_06635"
                     /inference="COORDINATES: protein motif:HMM:NF013661.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan recognition family protein"
                     /protein_id="HRX15971.1"
                     /translation="MMINRDYPCNPSNYANQADRTIEYIVIHYVGATGSALENVKYFN
                     RSAGLQASAHFFVGHESEGGAIYQSVDPLSRAWHCGAKVYKHPACRNSNSIGVEMCCH
                     KDAQGNWYFDDVTIEMTVQLVRQLMTEYGIPVDNVIRHYDVTGKLCPAPLTNEFAWLL
                     FKSRLEGPAMNEKTGDNPSSWAIPHTEWAKAKGIINGDGQGNYDWQGITTREAMAAML
                     HNFAITYGFEKEEKY"
     gene            complement(7948..8166)
                     /locus_tag="P5123_06640"
     CDS             complement(7948..8166)
                     /locus_tag="P5123_06640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009529288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRX15972.1"
                     /translation="MRAFSKFIVTMVIILNILFTLAILYLFWLKGQEPRFLIGAWFAF
                     TTGELWMLASIRNTKTKNPPEKVVKRDL"
     gene            complement(8163..8447)
                     /locus_tag="P5123_06645"
     CDS             complement(8163..8447)
                     /locus_tag="P5123_06645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011247659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRX15973.1"
                     /translation="MESITLGAIATVCSIAFGFIGYQRGVKKQCEDEGLQNGELKANI
                     SYIRAGVDDIKIDLKAQERRVGELAERVIRVEESSKQAHKRIDEIRGDCH"
     gene            complement(8644..10242)
                     /locus_tag="P5123_06650"
     CDS             complement(8644..10242)
                     /locus_tag="P5123_06650"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRX15974.1"
                     /translation="MAATLHIDAYTAVGSDIFVVPAGVTSVDVLVVAGGGAGYAGGGG
                     AGGLIFYEGYSVTPEDEISLSVGNGGTSASGSNSTFGTLTAIGGGRGGGWSSPYTGSN
                     GGSGGGAGNGSGNGGSGTTNQGNAGGNGNYWQGGGGGGGYYAVGSNGSGSTGGNGGIG
                     KYFGNIFGTDYGVSGWFAGGGGGWGNITKGSGGQGGGGDSGIKSDATKTNGTNGTTNT
                     GGGGGGSNATPGSGGSGIVLIRYITETDTKRTTKGKLLYKDFYNYAYSTNSNYTTHFG
                     ADETALAFNYTNRRVEDTSTGREITGGIYKNTTYENLYMRTTTKMLSGVNNDDWMGLG
                     FRRNNNSGAWYGSSSRLYIATIRKDGAIIFYKSISGTPTLLEGTDAGTFVPTTAEFDF
                     VIIANEDSFEILCDGSSKLTDTDVSITSAGYVEMIANSGVSACFKDLCICSSPDISCS
                     GLPSGYKLKVYDRDSNTRIATESGGTATVDGRTLMYPANKVEILNGSDTVVDTYEITG
                     DIWGGDVFKYTATPAMGILSATFV"
     gene            complement(10245..10934)
                     /locus_tag="P5123_06655"
     CDS             complement(10245..10934)
                     /locus_tag="P5123_06655"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRX15975.1"
                     /translation="MADVRGVCFSINNVDLATTQSVAITAPCALKVTGGKIEVGTAPT
                     GAALICDIHKNGTTLWTTQGNRPTVVPNALITALTGDNNDLLYQSKLDYTKYVSVVYV
                     DPEALTAACSAVRSGTGTSGDPYIVTVTLKHDGTVITATAADVKAAIEADADSNSTVI
                     CSNYSGNDGTGVVTALSATALSGGGVSASITAPDVTSIAQGDRLLLCIDQIGSTVAGK
                     DLSLTLYCEVV"
BASE COUNT         3185 a         2732 c         1780 g         3537 t
ORIGIN      
        1 ttctaagtct tcatcaatat aactctcagg catatttctc ctttcaataa tggaaaccgg
       61 cccaaggaat cgaactcaag gctacacctt tgtgctaagg gtgtagggtc ccagccgccg
      121 gttaaatgtt ggtatataga tggggaaaat ccataatatc cctatagaag ttccccgatc
      181 cgatagcgtt taatccatct cgcattcaaa ttttatcgta aataagccca tcgacattga
      241 aatcgagaag aatactatct ctataaccat ttacaaaatc ttgacgttcc tcagctatgg
      301 tgtcacagga atactcattt ctatactctg gtgtcgaaca attatatatt gaaaaatcaa
      361 taaagtcgtc accaccgcct cctaataccc aacctacgag agctccttct gtagatcttt
      421 taaagcctag agcgtcataa acttcattta aaaatacatg accgcgagaa tgaagaagtt
      481 gattaaagaa actttgttgg ccttttatat acgaaagatt ataatttgga gtatctgacc
      541 attcagaaga atatcctttt ccaaagaatc tagcatatac acttattcca ttaggatcaa
      601 ccacgtctac gttctttttt acttttttct tcttaccttt ttcgtcctct tcctcaacga
      661 ttatctcttg ttgccgcatt ccgagtctga atctcctgtc gacttcttca ccgtaatcct
      721 ctacaacacg cttacgatat ctcttgaacg catcatcgat agctgtgtaa gcagccataa
      781 gagcaatatt acgattacgc ataataatat gcgaaccgaa tatacaagca actgagacag
      841 cgcctaatac gactggtgga ccatataatt taacaagatc tacaccggct tgaaaggcaa
      901 ccaaaccacg atctttaata taatcattat ccgtatagtc tttattgttc gaaataagat
      961 gatcttttac ttcctcaatc tcagcgagtt ctgagtaata ttcttctttt atcttccgcg
     1021 ccttcggttc agctttagct atcttccaca cgctgtaact aagaccaaca acaccaagac
     1081 caatcataat ctctggggaa tttttcctta atatcaaccc aacacgacct aattttgtcg
     1141 gtataagact cttaacgagc gctaaactat tcatttatac cctcctcctt gaataactcc
     1201 atcatatttt nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1261 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn gctccatctc
     1321 aaaattcccc ttcctatctt aaaaaggaat attatcatag agataaatat aatcgataac
     1381 aaagttaatg ttatcatttt aaatcctccc tcaatttaag gacatatata ccttgtctta
     1441 ttgcggccgt aagagcatca tattttacag attcagcctc gtccctctca acctctttcg
     1501 aataagtgtt tcctttatta tcggtaactg tgacaataaa agcgtttatt tctttcttaa
     1561 acatgacgat tataacgtgc ttaccatccg ctagaagatc taaaacttca ttcattttag
     1621 cactccctct aatcaattaa ttttggtttc ggtaattcaa ggacgtaccc atctctatct
     1681 ctagttatga cggcattacg cagacttgtc caaccatatt tatcatctgt gaaattagtt
     1741 gaatcaattt ctacggcatc atatagatcg gcgatactca cggccccata ctcggtaatc
     1801 atatctacaa gatgatcccg tatctcctcg gcatcacgac gggaagaaaa tataatatct
     1861 tcaaaccctc ttcgtactct actatattgg cgttcttctc tatctcgccc acgatcatat
     1921 cttttatcat atggagtata aatagtccgc cctccacgtt cccttctagg tctatgccga
     1981 tcatcattaa ataacatcat gtcggtagca ccatttatca tatccgatat agtgctttta
     2041 gccgctggta ctaatatctc gttaagaaca tacgagccag caatagccaa accatcacca
     2101 aataagctat ctttgacacg ttgtagaaaa ggtttctttt ttaatataac ttttcctgaa
     2161 ataattttct ttactttttt ctcctctttc ttttccgtaa caaccctacg tgagttagag
     2221 ggataatccg aatttaatat tcgtacccct ctagagccat tatcaacttt aagcggttct
     2281 ctcttctctt catccataag tttatctcct tccaagataa tatattccta tttatatact
     2341 ggccattcgg cgccacaata tatacaatgt ctactttcgg catatccata aggataatta
     2401 tagtcaggac taccacattg ttcgcattct gaagctttta aacattttct tatttgagct
     2461 agactgcaaa tctctaattt tttctcgcat ttactacatg ctatagtttc ttccacgacg
     2521 ttccctccat aaaaaagaaa agataaatac cacgtttgtg atacttatct ttttccatta
     2581 cttctcgtta ggtttctctg tagtttcgac ttccagttcg ctcattacat gatcgatagc
     2641 tctaaacgtc ttcgagacat gacccacaac agcgtcagca gccatgcatg atattactat
     2701 ttttgctact ccaataaaga ttttacttcc gatctttatt tgcggtggca caacagaact
     2761 cactacctgc tcaactattg ccaaggttcc tatcccggca gttataccaa ctaccatgtc
     2821 agcaatgatc aattttttgt tagtcatttt acaaactccc tttcgaattt tatttcatta
     2881 tatgctgtgt aaatcctgcg agcttgtaaa tgcttttttc ttttatttag gccatgtgta
     2941 cttgacgaaa gccgcacata ttaaaataag cgcaaatgca caaaaaccgt aaatgagtag
     3001 tagtggtaat actaacttaa ggttctctat cataagccct ccagaagaat tttaatagta
     3061 ttaatatatt tagctaattc gtcatttatt ttttctattt ctcttcccca atgatcaatt
     3121 aatataattc tagcaagttt agaatcatca ttagtaagcc aaacaacgga atgatggact
     3181 ttagattctt cagtcgaaca tactaaacgt ctatatcttg ggccgctagg tctatcaact
     3241 cgtacaatga actcgccctt accatatcgg tctattctcg cataaccttt taaagtctct
     3301 aaattttgtc ttctcttaga tatacgatat aaatatgcag gtgtccctaa tgattccagt
     3361 gaatttacaa ccattttcaa tctcctttca agataaagaa aaagaaggat gttacgttaa
     3421 taacaccctt ctacacccat tgttaatttg aggattattc ctcagtggtt tcgacgatcg
     3481 ttgtttcgat gtaatcgatt tcttcgtcat catactcgtc gtcgtcaccg cacttgtttg
     3541 cgacagcttt cgctagtcct acaagagcga tacctcctaa gattgcaatt ccgatcttcc
     3601 aattactctt tacaaagttc aatacgttcc ccatgatttt attctccttt caaatatgtt
     3661 taataggttt cattataaca gatgtttttt tcgcgaatta atctgaaaaa tacgttggtt
     3721 cgctatcaat tactaaacat ggcgttccat gttgtgttag ttgagatgag aattttatat
     3781 ctatttgtcc tttactaata tcccaccccc aatattctcc tatagacgtc tctggtaaac
     3841 ctaattcata atataattcg ttcaatttaa cgaatccatc ggacaataaa agctcgttta
     3901 gattatttct taccctacgt atattctcta tatcgctctt aaaatatcgt cctgatgttc
     3961 tctcataaca aagcgtatct cccctacctg tgatagttat ctcgctaaca tctaccgggt
     4021 gtttctttat ttcttcttcg taaacctcgt cttcaatctt cttagctttc ttttccccta
     4081 ctacctcaac aactttattc tggtactctt ttagagttgt ctctgcgaga gtgtacatac
     4141 tagctaatgc tgcattacgc tttagattaa gggaattacc atagaatata caagcaagcg
     4201 tcacaccacc cattatcata gttggaatat aacaatgcca agtgatcttt ataacctctt
     4261 tttttgataa taaaggtttg ctagttagta cgcgataatt ataaccagaa tattttctgc
     4321 ggacctcctc ctcaattaac aatagagctt taggtgtggc ttttattcca agtatagctg
     4381 tagataccac acctgcacca ccaagagttg ccatgatagt tggtgagttt ttaacaagaa
     4441 atcgtccgat aaccttaatt aatggttttg gcatgacctc atcccttcca agaattttct
     4501 ttatttcttg gttttaataa cattaataat agcttttcct atagtgtagg cgccgacagt
     4561 agctgaaata ccaagcatta aagtacccat tttcccaagc tgaatcattc taaaacctcc
     4621 tttctagaaa aaataaaaag aagatatgtc ggaatcgaac cgactataaa taaacaccat
     4681 atatatcttc tattatacca tatgtttttt tcgcgaggaa aaaagggaag cctaagctcc
     4741 ctttttcaaa aaagtttcaa ttttcgaatt acctgcttta attaattcag ttattccgtt
     4801 accaattatg acacaaccaa ttacactaat agccatccct aaaagttccc ggttactaaa
     4861 tactattttt gcatagtttc ccattattat ccactccttt cattataagg agtgtttttt
     4921 tcgcgaatag aaaaaagaaa gggacctaag tccctgtaat taactctcat ttattttttt
     4981 actagagtta attttttgtt tcttaaatcc ttttcttttt ttaattcttc agtatcaatt
     5041 ccgaattcag cttttaccat ctcagttaat gtgtcagtca cgtccttaat accatctatt
     5101 cttcctcgat aatattgact tttgctatag caaaatccaa caaaccccag ataacccagt
     5161 gccaatgcgt gaaacataat tttccctccc atgatacttc tcctttcaag aatttatttc
     5221 attatatgat atgattttac cgcgagaaaa aaaaagagag agccgaagct ctcttaaacc
     5281 aatgctacac tatcattcaa tattaaacat acatatttat ctgcctcagc aaattcctta
     5341 aatattgcaa acatcgaatc gttattagcg tttaaatcaa gaagctcttt aactctctct
     5401 aacgtcataa atgtttctgg ctggttagca tcccaagtga tagcgttcaa cccacttgaa
     5461 ttaacaagat taagtccttt accatgctta attttcacga ttaatcctac aacactaatc
     5521 gttacaactg ttccgataat aatctctttc ttgtgatttt tgatgaattt tttgatatcc
     5581 atttagtatc tcctcctcca aaattttatt tcattatatg atatgatttt accgcgagaa
     5641 aaaaaaagag agagagcaat aaagctctct agaggttctt aattttatcc cagttatcca
     5701 tgatacctag aacgctacca acagccccac aagttccgaa tatagctaat ccgccaatta
     5761 tcccttgttt aaagcttcct tggcttaatc caacgatagc agctaatacg ataacccatt
     5821 cctggctcat ttgtattttc gctccgttca taaacacgtg accgataaaa ttgatcttct
     5881 ctaatttatt cattacgata tctcatagga atatcctgtt gttctgggtg atttttctca
     5941 gtccatatag caaaagctat attccacata gcagccctta aatgaggttc atccttcatc
     6001 ccacgtagat aacaagataa atggcgtacg gcactgtcta tcaagctatg tacaggtatt
     6061 cccttctcgc agttacgctc cccatatttg agagcgccct cctcacaatg tagggctaac
     6121 tcatgaatag catcccaggg aataagatca taccgaccct ttcctttatg taaatctctt
     6181 accgcaccag tctcatattc atgacgtttc ccgctatctt ttatcattac actcctccat
     6241 cttcggtaat atttgataga ataagtttaa ggttgaaaca agatgctcat ctattaattc
     6301 gtctaaagaa gtaaaatctg gggtgtaaaa catatgctga tttcttaata attcggcaaa
     6361 caataaagat aaatatgtta actcagctga ttcttttgcc agtagtctaa gtttttcttc
     6421 catgtaaaca cctccaatat agtaggaaaa aaggggaagg cgtgttttac acacgccctt
     6481 taagtacaaa ccctaaagct ttactagtta tgatgttaac cctctcgtat ccgaggatca
     6541 taccaattcc aatcagatta gtcattacaa tggcaatcgt gtccggtttt acttttcgat
     6601 ttttaatttc attattcatc ttatgtaacc gttctagatt gttcaatcta attaaatatt
     6661 ctgatgtggt tgcttcgatt cctcgcatct cgtctaagat taaatcgatc tccttctcaa
     6721 gtttagatct tctgtccttt ttggtgagtt tcaaatttac cttctccttt caaaatttta
     6781 tgtcttttca ttataacata cgttatttac gcgatttttc atcaaaaata acccgttttt
     6841 aggccaaatt gaaatagaga cgtctagaag cgtttctaag caattttacc ctttctgaca
     6901 tatgataggt ataggacagt attaaaaacc cttgagaagc gattctgata ggagtttttt
     6961 cttgaacccc ttgaaaagtt gattattttc ctatagcttg agcgtctatg taagattcac
     7021 ccaaaataaa tgcagttaca actccggtga tccacatata tgcttgttga tcgaccccaa
     7081 gacctaaacc ttcattacaa ataataaaca gagcagtaaa gaacgcggct agaaacttcc
     7141 tcgatttaag tctttcaata aaagatcttt gcattaatat ttttcctcct tttcaaatcc
     7201 gtatgttata gcgaaattat gtaacatggc agccatggcc tctctggttg tgataccctg
     7261 ccagtcataa ttaccttgtc catcaccatt aataatccct ttagccttag cccactccgt
     7321 atgaggaata gcccaagaac taggattatc gccagttttt tcattcatag ctggaccctc
     7381 caatctagat ttaaataata accaagcaaa ttcgttcgta agaggagctg ggcacagctt
     7441 gccggtcaca tcatagtgcc tgatcacgtt gtcaactggt atgccatact cagtcataag
     7501 ctgccgtacc agctggacag tcatctcaat agtcacatca tcaaaatacc agttgccctg
     7561 ggcgtccttg tggcagcaca tctcaacgcc gatactgttg gagttgcggc aggcgggatg
     7621 cttgtatacc ttggccccac aatgccaggc ccggctgagc ggatccacgc tttggtatat
     7681 ggccccgccc tcggactcat ggccaacaaa aaaatgagcg gaagcctgaa gccccgccga
     7741 tcggttaaag tatttgacat tttccagagc cgatccggtt gcgccaacgt aatggatgac
     7801 tatgtactca atggtgcggt cggcctggtt ggcatagttg ctggggttgc atggatagtc
     7861 ccggttaatc atcatcggcg tccaccccct cgggagcatc atcgccgaag cccctgtcat
     7921 cgtcatctat atacatggta tccctcctta taggtcccgc ttaaccactt tctcaggcgg
     7981 gttcttggtt ttggtgttgc gaatcgaggc gagcatccat aactcgccgg tggtgaaggc
     8041 gaaccaggcc ccgatcaaga agcgcggctc ctggcccttg agccagaaca aatacagtat
     8101 tgccagggtg aacaggatat tgagaatgat caccatggtc acaataaatt tagaaaaagc
     8161 cctcaatgac aatcacctct aatctcatct atccgcttgt gggcttgctt actgctctcc
     8221 tcgactctta tcacgcgctc cgccaactct ccgacccgac gttcctgggc cttgaggtca
     8281 atcttaatgt cgtccacccc ggctctgata tagctgatat tggctttgag ctccccgttc
     8341 tggaggcctt cgtcctcgca ctgctttttg acaccgcgct gataaccgat aaaaccgaac
     8401 gcgatggaac acaccgttgc gatggccccc agtgtgatag attccatttc ccacgcccct
     8461 ctcatagtga tatgtatctc aagcggcgcc ggaccgcgat tacaaaaagg taggcccgcc
     8521 gaagcgggcc atgaaaacga taaaagctta cgggcctaag tcattcacga cgtaggcact
     8581 attatgatgt tgggtgagtg tcggcagcgc aagtcatgga tcaccccctt accgacaggg
     8641 acttcaaaca aatgtcgcag ataatatacc catggctggg gtagctgtat atttaaaaac
     8701 atctccaccc caaatatccc cagtaatttc ataggtgtcc accactgtat ccgagccatt
     8761 tagtatttct actttattgg cagggtacat tagggttcta ccatccactg tcgcagttcc
     8821 acctgattcg gttgctattc ttgtattgct atccctatca tatactttca atttgtaacc
     8881 actgggcaat cccgaacatg atatatcagg ggatgaacaa atacacaaat ccttgaaaca
     8941 agccgataca cctgaatttg ctatcatttc aacgtaccca gcagaagtta tagacacatc
     9001 tgtgtctgtt aatttagaag aaccatcaca taaaatttca aaagaatctt cgttagctat
     9061 tataacaaaa tcaaattctg ctgttgtagg cacaaacgtt ccagcatcag taccttccaa
     9121 taaagtggga gtaccactaa tagatttata gaagattata gccccatctt tacgtattgt
     9181 agctatatat aacctagaac tactaccata ccatgcccca ctattattat tacgtctaaa
     9241 acctaatccc atccaatcat cattattaac accagataac attttggtag ttgtcctcat
     9301 atataaattt tcataagtag tatttttata tataccccca gttatttctc tccctgttga
     9361 cgtatcttct actcttctgt tggtataatt aaaagctaaa gcagtttcat ccgctccaaa
     9421 atgggtagta taattactat tagttgaata tgcataatta taaaaatctt tataaagcaa
     9481 ttttcctttg gttgttctct tagtatctgt ttctgttata tatctgatta atacaatgcc
     9541 agaaccacct gaacctggag tagcgtttga accaccaccg ccaccaccag tattagttgt
     9601 accatttgta ccattagttt tggtagcatc tgatttaata ccactatcgc cgcctccacc
     9661 ttgaccccct gaccctttag tgatattacc ccaaccacca ccaccacctg caaaccaacc
     9721 acttacacca taatcagtac caaatatatt cccaaaatat ttgccaattc caccattccc
     9781 accagttgaa cctgaaccat ttgaccctac tgcataataa cctccaccac ctccaccacc
     9841 ttgccaataa tttccattac ctccagcgtt gccttggttt gtagtccctg aaccaccatt
     9901 tcctgaacca tttcctgcac caccaccaga accaccattt gaaccagtat aaggcgaact
     9961 ccaacctcca cctctacctc cacctattgc tgtcaaagtt ccaaaagtgc tgttgcttcc
    10021 actagcacta gttccaccat taccaactga taaagatatt tcgtcttcag gcgtaacaga
    10081 ataaccttcg taaaaaatta atccacctgc accaccacca ccagcatatc ctgcgccgcc
    10141 accagcaact actaaaacat cgacagatgt tactcctgct ggaacgacaa atatatctga
    10201 cccaactgcg gtataagcat caatatgcag tgttgccgcc atgattatac cacctcacaa
    10261 tacagggtta gactcaaatc cttacctgct accgtgctgc ctatctggtc aatacacagt
    10321 aagagtctat ccccttgcgc tatgctggtc acatcgggcg cggttatgct tgcgcttaca
    10381 cccccgccag ataacgccgt agctgatagc gcagtcacca cgccggtacc atcgttaccg
    10441 ctataattgg agcaaataac cgttgagttg ctgtcggcat ctgcctctat cgccgccttt
    10501 acgtcagccg ccgttgctgt gataaccgtc ccatcatgct ttagcgtgac agtaactata
    10561 taaggatctc cagatgtccc ggttcccgac ctaaccgccg aacatgccgc agttaatgct
    10621 tcgggatcaa catatacaac gctgacatac tttgtatagt ccaatttcga ttggtacagc
    10681 aagtcattat tgtcaccagt taaggctgtt attaatgcgt ttggaacaac ggttggacgg
    10741 ttgccctgcg tagtccatag agtagtcccg tttttgtgta tgtcacaaat tagcgccgcc
    10801 ccggttggtg cggtgccaac ctcgatctta cccccggtca cttttagtgc gcatggtgcg
    10861 gtaatcgcta cgctttgagt agttgccaaa tccacattgt taatactaaa acagacaccc
    10921 cggacatctg ccacccactc aggcgcggtt gccccggcat tgacggctag tatctgccct
    10981 gctgtcccga tccccagcct cgcctgggat gatgcacctt gaatgaggat gtcaccctgt
    11041 gaagtcagca ccccatcggt aatcccaacc tcgacccata ctggccccgt ggcattagtg
    11101 catttataca gcttgccagt atcggcttgt aatagcagtt gccccaataa tgggacataa
    11161 gccgcctttg cggtagcatc tgccaccacc ttgattccgg gatgcgcctg tattacatcc
    11221 agtatagtat tgagttccgt accccatgta ccgcttgatc caccaactgt cggtttggcc
    11281 caatcaaaat cagccaacac atatcacctc ccctgtgtag tcgctggcca gcgt
//
LOCUS       DAOTGZ010000076        11270 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_3064
            NODE_6867_length_11270_cov_4.489523, whole genome shotgun sequence.
ACCESSION   DAOTGZ010000076 DAOTGZ010000000
VERSION     DAOTGZ010000076.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868088
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 11270)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 11270)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:22:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,785
            CDSs (total)                      :: 2,751
            Genes (coding)                    :: 2,732
            CDSs (with protein)               :: 2,732
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 12 of 19
            Pseudo Genes (internal stop)      :: 5 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11270
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6867_length_11270_cov_4.489523"
                     /isolate="AMR_MDS_3064"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(135..389)
                     /locus_tag="P5123_06660"
     CDS             complement(135..389)
                     /locus_tag="P5123_06660"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRX15976.1"
                     /translation="MTHKVIGVLVTDRVKEAGAVQDTLTKHGCIIRTRLGLHQAGNHC
                     SPDGLLLLELAGDINEWVELEEDLKTIEGIQVQSMSFNQP"
     gene            923..2050
                     /locus_tag="P5123_06665"
     CDS             923..2050
                     /locus_tag="P5123_06665"
                     /inference="COORDINATES: protein motif:HMM:NF024206.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ankyrin repeat domain-containing protein"
                     /protein_id="HRX15977.1"
                     /translation="MKSLKKFKNEAEKHVEDMDKLSEIIGEIANGDFPEEYYLFACEL
                     CDLLLGRNASDHKTRYRRGYLLFKANNFEEATEEFTYVINNDPKRSSAVLYDRARAYY
                     QNGLFEESLVDIERILSEYKQFVEEGFSLDILHVLRGMIFIKTDKTDLAIEEFEKAEQ
                     YAPDDENISTMVESAKYELSQKNIFLNLKKLLRKDETESFIEAFGSLNNPNYTILSDK
                     GMTLLHHAAELGNIELVLFLLETGANPDSVSDDNLYPVHIAALHDKTETIVLLIKHGA
                     NMYMPSSIEGGSLLNYLIIHKRLDDLCALVAEGADINRIIPGVPAPLHTAVSSNNLDA
                     VKKLIELGADVNIEDSDGVTPFDIAIDNGFIKIEDLFRELL"
     gene            2312..6847
                     /gene="gltB"
                     /locus_tag="P5123_06670"
     CDS             2312..6847
                     /gene="gltB"
                     /locus_tag="P5123_06670"
                     /EC_number="1.4.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010262353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0015930 - glutamate synthase activity
                     [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_process: GO:0006537 - glutamate biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0006807 - nitrogen compound metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate synthase large subunit"
                     /protein_id="HRX15978.1"
                     /translation="MLQMYPDNQGLYDSNNEHDACGIGLVANIKGKRSHDIVRRGLEV
                     LERMEHRGAEASDNKTGDGAGIMIQIPHELYSREIAALPTAGNYGTGLLFLPKDEKDR
                     DNIKKIFEEIVKTEGAKILTWRQVPVNSSEIGEIASRTEPHVEQVFIVPGDGQQQQQL
                     EQQLYIIRKVSESEISKNALYKKSIFYVVSLSSKTIVYKGMLMPGQVSAYYSDLKDEK
                     CLSAAALVHSRFSTNTFPAWDLAQPFRMIAHNGEINTVKGNRFWMRAREPFLKSDAFG
                     DDISKILPVVEGGKSDSASFDNVLEMLVSAGRPLPHALMMMVPESWNDKNPISDELKK
                     FYEYHAAMMEPWDGPASLVFFDGRFLGGTLDRNGLRPSRYTITDDDLIVMGSETGVQD
                     FEVQNVRYKGRLMPGKLLLVDIQEGRIIPDFEIKQMIVNSKPYKQWVMENRITMEDLP
                     DVTSFSDDISKEKVQGLFQLYGYSREESDKLLPALAIDGQEITSSMGTDTPIAIFSDI
                     PQRLYSYFKQVFAQVTNPAIDPIREELVMTLTSFLGRQKNLLDETPEHCRMLKILNPV
                     FTNKDLAKIKRFENPDFTSVVLDAVFPAADGAAGLEKALEKFYLLAEEKIKEGATFLI
                     ISDKTVDENLVPIPSLLASAGLHHHLIRKQLRTQCSIIVESAEPREVQHFCLLFGYGA
                     DAINPYGAYAMLKSLVLDSKINIDYEKAVYNFNKGISKGILKVLSKMGISTLRSYRGA
                     QVFEAVGLGNELVEKCFTGTPSRIGGIGILQLAEEVLESHKQAFVKKPSLLFSEGMYK
                     WRRDGEQHAWNPETIHMLQWATRTGDYTKFKKFTSLVNDYNRKPNVIRGLFDFKKTDP
                     IPVDQVESEDEIMKRFTTGAMSFGSISKEAHETIAKAMNKIKGRSNSGEGGEDPARFT
                     PDKDGMLARSAIKQVASGRFGVTSNYLANADEIQIKLAQGAKPGEGGQLPGHKVDIKI
                     AATRHSTPGVTLISPPPHHDIYSIEDLAQLIFDLKNANPNARVSVKLVSETGVGTIAA
                     GVAKAHADNILISGYDGGTGASPQSSIRYAGLPWEIGMAETHQTLVLNDLRGRVRTQT
                     DGQLKTGRDIVIAGMIGAEEFGFGTSVLIVLGCMMMRKCHLNTCPFGVATQDPELRNK
                     FMGKSDYLINFFRFLARDVRETMASLGVSKFDDLIGRTDMLVQREIDHPKASFVDLSA
                     IIYRPAESDKYDVYCKKDQVHKTESVLDRKILSDASRALDSGEKVNLEYNIKNTDRTM
                     GTMLSHAVSQKYGESGLPSNTVNVIFRGSAGQSFGAFLARGLSFKLEGDANDYFGKGL
                     SGGTLVAVPPAGSKFAAEESIIIGNTVLYGATSGEAYINGVAGERFCVRNSGALAVVE
                     GTGDHCAEYMTGGRLVVLGSVGRNFAAGMSGGIAYVYDKSGDFDFYCNKGMVELTRVG
                     DAQDQQFLLETVQKHFEYTKSAVAGRILENWYASLGNFVKVMPLEYMRALNEMKIAEI
                     NKKLVGVTEEEELGRSV"
     gene            6847..8271
                     /locus_tag="P5123_06675"
     CDS             6847..8271
                     /locus_tag="P5123_06675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002771451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate synthase subunit beta"
                     /protein_id="HRX15979.1"
                     /translation="MGDPKGFLKVKRREGGYRPVEERINDYHEVEIQLDESGRREQAS
                     RCMDCGVAFCHWSCPLGNIMPEWQDALYRGDWKYAYEILQQTNNFPEFTGRVCPALCE
                     ASCVLAVNDEPVTIRQNEWFVIDKAWELGLVKPRVPQNRTGKKVAVIGGGPAGLAAAD
                     NLNQLGHTVTLFEAEANVGGFVRYGIPDFKLDKKVIERRIGLMEEEGVVFKTSTRVGK
                     DIASESLLKDYDAVCITIGARKPRDLVCEGRDTGGIHMAYDYLSQQNKSCSGEKVCRE
                     SLIQALDKDVVVIGGGDTGSDCVGTANRQGARSVYQLELLPQPPAHRTEDMPWPLWPR
                     LHKVSSSHKEGCVRLWSVSTKRIESDSEGNVKKIHGVKVQWDKDENGRFVMKEVPGSD
                     FTLNADLVLLAMGFVHPEHEGPVNELGLEKDERGNIKVDASFKTSVDSVFAAGDARRG
                     ASLVVYAIDEGRRVATAIDQYLLS"
     gene            8722..9021
                     /locus_tag="P5123_06680"
     CDS             8722..9021
                     /locus_tag="P5123_06680"
                     /inference="COORDINATES: protein motif:HMM:NF014087.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhbY family RNA-binding protein"
                     /protein_id="HRX15980.1"
                     /translation="MKLTGKQRGYLRKLAQELDPCIMIGKSGLSEQLIKATDEALDHH
                     ELIKVRFSDFKDEKKDLSLKLAQLTNSQIIGQIGHVAMLFRQNSDLEKRKIKIIE"
     gene            9146..9784
                     /locus_tag="P5123_06685"
     CDS             9146..9784
                     /locus_tag="P5123_06685"
                     /inference="COORDINATES: protein motif:HMM:NF012876.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0098561 - methyl accepting chemotaxis
                     protein complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein"
                     /protein_id="HRX15981.1"
                     /translation="MPKKSSLRKRMIFYFILIAFANVFVAGEIIFEISSDKYRQNVYS
                     SIDTPQQSERVQVVDNMLDQLVKKYIVLVSILILVSSVILFLFVVQIVSPLNYMINMA
                     AKMSEGDLSLRVKMRSNDELSDLGNLINDLSNNVQEIIAQVERISFDMTKLCALHSKN
                     LKRHSKFPQIFKSETDAMHLISQNLLLLKNSYVLYAIESFKHNYLDKENTQL"
     gene            9781..10554
                     /locus_tag="P5123_06690"
     CDS             9781..10554
                     /locus_tag="P5123_06690"
                     /inference="COORDINATES: protein motif:HMM:NF014035.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite exporter TauE/SafE family protein"
                     /protein_id="HRX15982.1"
                     /translation="MIEYIWLIPVGMIISTFASVIGIGGGLIWAPFFILLLGMEPQHA
                     AMYSFAVQIVGMGSAAITNILHKNIYWKLVFFLMPLIFIGELAGSFINQRIASPDVLQ
                     GAMGVMSIFVSLFFAFQPERYDAVLNEDRKTKPPCWMMGLCIPFGSFSGLFSVGIGDN
                     LVPLFRSKLKISMTNAVGTCLFLNLVIALVGLVLHLCLSGNNFGFLNLDILVMCWIGV
                     FVGGQVGPRVSGSIGDNRLKEMFIFILMLIGIHLIYKAM"
BASE COUNT         3273 a         2070 c         2525 g         3402 t
ORIGIN      
        1 tgttaatctg tcagcaggca tgagcaaagt gcatacaaca tccgagtaca taaaaaaaag
       61 tgatcttgta aaactatcac gccttgtaac ggctctaatt gacaaagcct aatctaataa
      121 ttttttctaa ggtttcaggg ctggttgaaa gacatgctct gaacctgtat tccctctatt
      181 gtcttaaggt cttcttcaag ttctacccac tcgttgatgt ctcctgcaag ttcaagaagt
      241 aaaagtccgt cgggagaaca gtggttgcca gcctgatgca gtccaagccg tgtgcgtata
      301 atacatccgt gtttagtaag tgtgtcctga acagcgccag cctcttttac gcggtctgtt
      361 acaagaactc caataacttt atgtgtcata atataactcc tttaacctct ttttgttcga
      421 aatttattaa caactaattg aacgataaaa aagtctggtc taaaaattac aacatgatgt
      481 taatcaagtc gggcttgtct tccaaaaaca cgcaaataaa actttttatt ataatcgcaa
      541 tttctaatca aataccgtaa aagtgtaatt ataacctaaa actgtatatt cagtcaacaa
      601 aaatctcgat atatactttg aataagcttt attttttaac ctcctttgat aatcatttta
      661 tacacttgac aaaattaaaa taggtgtaat ttatattgta caaatgaaaa gcttttaagt
      721 tttagctgtt tcggataaca aattttaatt tctagtgata tttccggctt ttttaaagta
      781 atttttgcag gttcctctgt ttgcttgtca ggcaccactt ataaaaggta aagaggtgct
      841 aggctgcttg caaataatat gttatactag ttgataaact aattatcaga gaatatgaaa
      901 ttattaataa acggaattga taatgaaaag tcttaaaaaa ttcaaaaacg aagcggaaaa
      961 acatgttgaa gacatggata aactttccga aataattggt gaaattgcaa atggtgattt
     1021 ccctgaggaa tattatcttt ttgcttgtga attatgcgat ctgctgctgg ggcgtaatgc
     1081 ttctgatcat aaaacacgct acaggcgtgg ttatctactt tttaaggcaa acaacttcga
     1141 agaagctaca gaagagttta cgtatgtgat aaataatgat ccaaaaagaa gctcggcggt
     1201 tctttatgat agagcccgtg catattatca aaacggcctt tttgaagaat cgcttgtcga
     1261 catcgaacgg atattaagcg aatataaaca gtttgtcgaa gagggttttt ctctggatat
     1321 tcttcatgtt ttacgaggca tgatttttat aaagacagat aaaactgact tggctataga
     1381 agagtttgaa aaggccgaac aatatgcacc tgacgatgaa aatatatcca cgatggtcga
     1441 atctgccaaa tatgaattaa gccagaaaaa tattttctta aaccttaaga agttactacg
     1501 aaaagatgag acggaatctt ttattgaagc cttcggttct ctaaataatc caaactatac
     1561 gatactatct gataagggta tgaccctgct tcatcatgca gccgaacttg gcaatataga
     1621 acttgtgcta ttcttgctcg aaactggcgc taatcccgat tcagtttctg atgataattt
     1681 atatcctgtg cacatcgcgg ctttgcatga taaaactgaa acaatagtgc ttctgattaa
     1741 acatggcgcg aatatgtata tgccttcttc catagaaggg gggtctcttt taaactatct
     1801 gataatacat aaaaggctgg atgacctctg tgcgcttgtt gcagaaggag ccgatattaa
     1861 tcgtattatt ccaggagtgc cggctccgct acatacggct gtttcatcta ataacttgga
     1921 tgctgtaaag aaactaatcg agttgggcgc ggacgtgaat atcgaagata gtgatggagt
     1981 tacaccattt gacatagcta ttgataatgg atttataaaa attgaagatc tatttcgtga
     2041 attgctttag ttgatttctt ccctaaaatt ttgtattata gcctgaatac atgtacaata
     2101 atctggtaac aaagactata agaaacatct gttaaaactg gttattttac tcaaacaaaa
     2161 ccattaatcc accaccatta actaatttta cttttgtgta acattacagg ctttgcaacg
     2221 gatctttttt gttgtacttt gagtatgcct gcccaatcgt ggcatatgca ggtttgaaat
     2281 aaacctgcaa gtatatataa ggagactgcc gatgttacaa atgtatcccg acaatcaggg
     2341 gctgtatgac agtaacaatg aacatgatgc ctgcggtatc gggcttgttg ccaatataaa
     2401 aggcaaacgt tcccatgata tagtacggcg cggccttgaa gtactcgagc gtatggagca
     2461 caggggagcc gaagcttctg ataataaaac aggagacggt gctggtatca tgattcaaat
     2521 accgcatgag ctttattcgc gtgaaatagc cgctctgcca actgctggta attatggtac
     2581 tggactactg tttttgccta aagatgaaaa agatagagat aatataaaaa aaatatttga
     2641 agaaattgtt aaaactgaag gcgcaaaaat tctaacttgg cgtcaggttc ctgtaaactc
     2701 tagcgagata ggagagatag catcacgtac cgagccacat gtagaacagg tgtttattgt
     2761 tcccggagat ggtcagcaac aacaacagtt agagcaacag ttatacataa tccgaaaagt
     2821 gtcggaaagt gaaattagta aaaatgctct ttataaaaag agcatttttt atgtcgtttc
     2881 actttcctca aaaacaatag tttataaagg aatgcttatg ccggggcagg tttcggctta
     2941 ctattcggat ctaaaagatg aaaaatgctt aagtgcggct gctctagtgc attcacggtt
     3001 ttctaccaat accttccctg cttgggatct tgcccaaccg tttagaatga tagcgcataa
     3061 cggtgagatt aatactgtca aaggtaaccg cttttggatg cgcgccagag agccattttt
     3121 aaaaagtgat gcttttggtg atgatattag caaaatattg cctgtggtcg aaggcggcaa
     3181 gagtgattct gcgtcatttg ataatgtgct cgagatgctt gtatctgcgg gacgtccttt
     3241 accccatgct cttatgatga tggttcccga gtcatggaac gacaagaatc caatcagtga
     3301 cgagttaaaa aagttttatg aatatcacgc ggccatgatg gagccatggg acggtccggc
     3361 atcacttgtg ttttttgatg gccgctttct tggtggaact cttgaccgaa atggtttgcg
     3421 gccatctagg tacacgataa ccgatgatga cctcatagta atgggctctg aaacaggtgt
     3481 tcaggatttt gaagtgcaga acgttcgcta taaagggcgg cttatgccag gtaaacttct
     3541 tcttgttgat attcaggaag gacgaattat tcctgatttc gagatcaaac agatgatcgt
     3601 aaacagtaag ccctacaaac agtgggtaat ggaaaacagg attactatgg aggatcttcc
     3661 tgacgtaacg tcttttagtg atgatatttc caaagagaag gtgcaaggtc ttttccagtt
     3721 gtatggttat agccgtgaag agtcagataa acttttacca gctctcgcta tcgacggcca
     3781 agagataaca tcatcaatgg gtacagatac gcctattgcc attttctccg acattccaca
     3841 aagactttac agttatttta aacaggtttt tgctcaggtt actaatccgg ctattgaccc
     3901 gataagggaa gagctggtta tgactttaac cagttttcta ggtcgccaga aaaatctttt
     3961 ggatgagaca cctgaacatt gccgtatgct taagatattg aacccggtat tcacaaacaa
     4021 ggatctcgct aaaattaagc gatttgaaaa tccggatttt acaagtgttg tacttgatgc
     4081 ggttttcccg gctgccgacg gagctgccgg actagaaaaa gctttggaaa aattctacct
     4141 gcttgcggag gagaagataa aagagggtgc aactttctta ataatatctg ataaaaccgt
     4201 tgatgaaaat cttgtaccca taccttcgct attagcatcg gcaggtcttc atcatcacct
     4261 gatccgcaaa cagcttcgta ctcagtgttc gataattgtc gaatctgcag aaccacgcga
     4321 ggttcagcat ttttgtctgc tattcggtta cggggcggat gcgattaatc catacggagc
     4381 atacgcgatg ttaaagagcc ttgtcttaga ctccaagatt aatatcgatt acgaaaaggc
     4441 agtatataat tttaacaaag gtatctccaa gggcattcta aaagtgctct ccaaaatggg
     4501 gatatcgact cttcgctctt acaggggagc tcaggtcttt gaggccgtcg ggcttggcaa
     4561 tgaacttgtt gagaaatgct ttacaggaac accgtcccgt ataggtggaa tcggtatttt
     4621 gcaacttgcc gaagaggtct tggaatcgca taagcaggct tttgttaaaa agccgtcact
     4681 cctttttagt gagggtatgt acaagtggag acgtgatgga gagcagcacg catggaatcc
     4741 tgaaacaatt catatgctcc agtgggctac ccgtacagga gattacacca agttcaaaaa
     4801 attcacatct cttgtaaatg attataatcg aaaacctaat gttatacgtg ggcttttcga
     4861 tttcaaaaaa acagatccaa taccggtaga ccaggtcgaa tctgaagacg agatcatgaa
     4921 gcgttttaca actggagcca tgtcgtttgg ttccatctct aaagaggctc atgaaacaat
     4981 cgcaaaggct atgaataaaa taaagggccg ctcaaattcg ggagagggag gagaagatcc
     5041 ggcaaggttt acgccagata aggatggaat gcttgcccgt tccgcgatca agcaggtggc
     5101 ttccggccgt tttggtgtga cttccaacta tcttgccaat gccgatgaga ttcagataaa
     5161 actggctcag ggtgcaaagc cgggtgaggg tggtcagtta ccagggcata aagtggatat
     5221 aaaaattgct gccacacgtc actcaactcc gggggtaaca ttgatatctc cgccgcctca
     5281 tcatgatatc tactctatag aagatctggc acagctgatc tttgacctaa aaaatgccaa
     5341 tccaaatgcc cgtgtttcgg tcaaacttgt tagcgaaact ggagtaggaa ccatcgctgc
     5401 cggcgtggcc aaagcccatg ctgataatat tctgatttcc ggttatgatg gtggaacagg
     5461 ggcttctccc caaagctcaa tacgttacgc agggcttcct tgggagatag gcatggctga
     5521 gacccatcag acacttgtgc taaacgattt acgggggcgt gtgagaaccc agactgacgg
     5581 ccagctaaaa acgggtcgtg atatagttat tgccggcatg ataggagccg aagagttcgg
     5641 tttcggaacc tctgttctga tagttcttgg ttgcatgatg atgcgtaaat gccatcttaa
     5701 tacttgtccg tttggagttg caacacagga tcctgagctt cgtaataaat ttatgggcaa
     5761 aagtgattat ctgatcaatt ttttccgctt tttggctcgt gatgtgcgcg agaccatggc
     5821 ttctctggga gttagcaaat ttgatgatct gattggacgt accgatatgc ttgttcaacg
     5881 tgaaattgac catccaaagg catcgtttgt cgacctgtcg gcgataatat atcgaccggc
     5941 cgaatcagat aaatacgatg tctactgtaa aaaagatcag gttcacaaaa ccgaatcggt
     6001 tcttgaccga aagattctgt ctgacgcatc aagagcatta gatagtggtg aaaaagttaa
     6061 tcttgagtac aacataaaaa atacagatcg aactatgggc actatgcttt cgcacgctgt
     6121 ttcacaaaaa tatggcgaat cgggtcttcc ttcaaatact gttaacgtga tctttcgtgg
     6181 gtcagcgggt caaagtttcg gggcttttct tgcgcgcgga ttaagtttta aacttgaggg
     6241 tgatgccaat gactatttcg gaaaagggct ttctggagga acgcttgtgg cggttcctcc
     6301 tgcaggttca aaatttgcag ccgaagagag cataattatt ggtaatactg ttctttacgg
     6361 tgcaacttcg ggtgaggcct atattaacgg agttgctggc gaaagattct gtgttcgtaa
     6421 ttcgggtgca cttgctgttg tcgagggtac gggagaccat tgtgccgaat atatgacagg
     6481 cggtcgtctt gttgtattgg gttcggttgg gcgtaacttc gctgccggta tgagtggagg
     6541 catagcttat gtttatgaca agtctggtga ttttgatttt tattgcaaca agggtatggt
     6601 cgagttaact cgcgttggtg atgcccagga tcaacagttt ctgctcgaga cagtacagaa
     6661 gcattttgaa tacacaaaga gcgctgttgc cggacgaata cttgaaaact ggtatgcctc
     6721 actcggtaat tttgtaaaag tgatgccgct tgaatatatg cgtgccctga acgagatgaa
     6781 aatcgcggaa atcaataaaa aactcgtagg agtcacagaa gaagaagagt tagggaggtc
     6841 tgtataatgg gagatcctaa aggtttttta aaagtaaaaa gacgagaggg cggttatcgt
     6901 cctgttgaag agagaataaa tgattatcat gaagttgaaa ttcagcttga tgagtctgga
     6961 cgtagagagc aggcttctcg ttgcatggat tgcggtgttg cattttgtca ctggagctgt
     7021 ccacttggta atataatgcc ggaatggcag gatgctcttt atcgaggaga ctggaagtat
     7081 gcctacgaga tacttcagca gacaaacaat tttccagaat ttacaggtcg agtctgtccg
     7141 gctctttgcg aggcgtcttg tgtgcttgcc gtaaatgatg aaccagttac tattcggcaa
     7201 aacgagtggt ttgtaattga taaggcctgg gaactgggtc tagtaaaacc tcgggttccg
     7261 caaaaccgta ctggtaaaaa ggtcgctgta attggcggcg gtcctgctgg tttggcggcc
     7321 gcagacaatc tgaatcagct ggggcatacg gttacacttt tcgaagccga agcaaacgta
     7381 ggcggctttg ttagatatgg aattcccgac tttaaacttg ataaaaaggt catcgagcgc
     7441 cgtatcggac ttatggaaga agagggcgta gttttcaaga catctacccg tgttggtaaa
     7501 gatatcgcgt ccgaatctct gttaaaggat tacgacgcag tatgcataac tataggcgca
     7561 agaaaaccac gcgacctcgt atgtgaaggc cgcgacaccg gtggtattca catggcgtat
     7621 gattatcttt cacagcaaaa taagagctgt tcgggagaga aagtttgtag agagtctctt
     7681 attcaggcgc tggataagga tgtcgtagta atagggggag gtgacaccgg aagtgattgc
     7741 gttggtactg caaaccgtca gggtgcacgt tctgtttacc agttagaact cttacctcaa
     7801 cctcccgctc accgtacgga agatatgccg tggccacttt ggccaagatt gcataaggtt
     7861 tcctcttcac ataaagaagg ctgtgtgagg ctctggtcgg tgtcgacaaa aagaatcgag
     7921 tctgacagcg aaggaaatgt taaaaagata catggtgtca aggttcagtg ggacaaagat
     7981 gaaaacggac gtttcgtgat gaaagaggtt cccggatccg attttaccct taatgccgat
     8041 ctggttctgc ttgcaatggg ttttgttcat ccagagcatg aagggccagt taacgagctg
     8101 ggtcttgaaa aagatgagag gggtaatata aaggtggacg catcgtttaa aacaagtgtc
     8161 gactcggtct tcgcggccgg cgatgcccga cgtggagctt cgcttgtggt ctatgctatt
     8221 gacgagggca ggcgtgtcgc aacagctata gatcaatatc ttctatcttg agtatttatt
     8281 gttaaggaaa tagttcatgt gtgctgttat gcttattcgt gcacaacagc acacaatttc
     8341 ttttttaaat ttgtaacttt tattaatttt atatctgtat tcatcttcag aattatatgg
     8401 ataataagtc aagaatgcct cccttttgat aaaacaaagt ttctatccca aataactccg
     8461 tccccattaa taaaaatata atatttctta aagtaggtca aattttagat ttttccgaat
     8521 tgaagttttt atggaattta atatagtatt gaggtgaatt taagttctaa ctacttttat
     8581 tatcaaataa tgggttgact caataagcta aaatagattc aatctcaaac cgataagaag
     8641 taatttatcg tataaaatgg taaatcagta aggctgaatc gattaacttt ggctttatat
     8701 agagtaaata aaggtgattt aatgaaatta acgggaaaac agagaggata tttaagaaaa
     8761 ttggcacagg agcttgatcc ctgcattatg ataggtaaat ctggactatc tgaacagttg
     8821 atcaaagcca cggacgaagc tcttgatcat catgagctta tcaaggttag gttttctgac
     8881 tttaaagatg agaaaaaaga tctgtctttg aagttggcac aacttactaa ttcccagatt
     8941 attggacaga taggtcatgt tgcaatgctt tttaggcaaa atagcgattt agaaaaaagg
     9001 aaaataaaaa ttatagagtg attgtatcaa taataatatt cttgacggat tttttttgtt
     9061 tctttagatt ggggcaacat gattggaaac tggtttccct ctgtagctgt aaaacttccg
     9121 gatatttaat gaaggacaca attatatgcc caaaaaaagc tcgctgcgca aacggatgat
     9181 tttctatttt atattaatcg cttttgctaa cgttttcgtt gccggtgaga taatatttga
     9241 aatatctagc gataaatacc gtcagaatgt ttatagcagt atagataccc cccaacagag
     9301 tgaaagagtc caagttgtag ataatatgct tgatcagctt gtaaaaaagt atattgtgct
     9361 cgtatccata ttaattcttg tttcatctgt aatactcttt ctgtttgtag ttcagatcgt
     9421 ctcaccatta aactacatga taaatatggc cgccaagatg tcagagggag accttagtct
     9481 gcgagtcaag atgcggtcta acgatgaact ttcggatctg ggcaatctga ttaacgatct
     9541 ctctaacaat gttcaagaga ttattgccca ggttgaacgt ataagttttg atatgacaaa
     9601 gctttgcgca ttacattcaa aaaatctgaa aaggcatagt aaatttcccc agatattcaa
     9661 gagtgagacc gatgcgatgc acctgatttc gcaaaatcta ctgttactta aaaacagtta
     9721 tgtgttatat gccatagaat cttttaaaca taattatctt gataaggaaa acacgcagtt
     9781 atgattgagt atatatggtt aattcctgtc ggaatgatta tttctacctt cgcgtctgta
     9841 attggtattg gtggtggtct tatctgggct ccgtttttta tacttctttt gggtatggaa
     9901 ccacagcacg ccgcgatgta ttctttcgcg gtgcagattg ttggaatggg gtcagctgcc
     9961 ataaccaaca ttctgcataa gaatatatac tggaaacttg tatttttctt gatgccgtta
    10021 atatttattg gtgagcttgc cggctctttt attaaccaga gaatagcttc tccagatgta
    10081 ttacaaggtg caatgggggt gatgagtatc ttcgtcagtc tcttttttgc gtttcaaccg
    10141 gaacgttacg acgctgtact taacgaagac cgaaaaacga aacctccttg ctggatgatg
    10201 gggctttgta ttccttttgg tagtttttcc ggtctttttt ctgtaggtat cggcgataat
    10261 cttgttccgc ttttccgcag caaattaaaa atatctatga caaacgcggt gggtacatgt
    10321 cttttcctta atcttgttat tgctcttgtt ggtcttgtgc tccatttatg cttaagcggt
    10381 aataattttg gttttctgaa tcttgatatt cttgtaatgt gttggatagg agtctttgtc
    10441 ggtgggcagg tcggcccaag agtaagcggt tctatcggtg ataaccgtct taaagagatg
    10501 tttatattta ttctgatgct tattggtata catcttattt acaaggcgat gtagcgtatt
    10561 tcaattttcc cttgcggatt cctattattt tacattaacc agcgagacgc actgcagtgc
    10621 gtctttacga ttctgccatt ttctttcctt tctttctctt ccagagagtg gatttacttt
    10681 tagtaaagca ataacctttc tgttctttct ttttatggca aaaagaaaga accaaagaaa
    10741 atgccacaag gggctgctgc ccctcgaccc cgcaaggtga ggattattaa taatattctt
    10801 aaaggtttta tggctttgat atttatacgt aatttctgct tttgaatgtc cgcttagctg
    10861 gcatcctgcc agaagcgcta ctgcgccggc atcgtgccgg ctttagagta cctgatatgg
    10921 aagttctgat tttatggatt ttttctaaac ggtttttgga ttttcagcta acagacaact
    10981 caacttagaa ataatttcat cctggtgtca ttccggccga agcgaagcgg agtgccggaa
    11041 tcccgggtga tgactaattc ctaatatgga ttcctgcatg cgtaggaatg acaatatacg
    11101 gattcgtatt gatttacacc gtccctattg tttacacggc cgggcgtctt tacgatgctg
    11161 ttatcgtttt gtttttttat gcgggaattt tgctgtttgt tgatcttaca ttgccttttc
    11221 ttcgcccttt ttttcaaaaa atcagttgtt ataggtaagg gaaaaggagg
//
LOCUS       DAOTGZ010000077        11216 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_3064
            NODE_6905_length_11216_cov_4.489831, whole genome shotgun sequence.
ACCESSION   DAOTGZ010000077 DAOTGZ010000000
VERSION     DAOTGZ010000077.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868088
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 11216)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 11216)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:22:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,785
            CDSs (total)                      :: 2,751
            Genes (coding)                    :: 2,732
            CDSs (with protein)               :: 2,732
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 12 of 19
            Pseudo Genes (internal stop)      :: 5 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11216
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6905_length_11216_cov_4.489831"
                     /isolate="AMR_MDS_3064"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..2508
                     /locus_tag="P5123_06695"
     CDS             <1..2508
                     /locus_tag="P5123_06695"
                     /inference="COORDINATES: protein motif:HMM:NF013069.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HRX15983.1"
                     /translation="VLRMKGQYTGVEEVKKQVIRANDGGFETTLMDIAKVSDTFEEPE
                     IIERINGKNAIVLEVFKKRSMDEITLATNIKENIDSFDSPFPDDVNILIFNDSSEFTE
                     KTIDTVINSAVTGFFLCALVLLIFLGPRMASLVTSTIPLVFLVAFIFMSILDVNLNVI
                     SLFGMIMVLGMIVDFGIVITENCHRYIEMGLEKGEAILTGVCEIFKPVTVSFICISAS
                     FVPLLVLTGLMGKFIKNIPLMILICLSASWIVALFFMPTFLKMFSKEEEKVHKELETE
                     DGEHLEKGFMGRFQFAYIGFIRQALRFRYITVAILVAVLGGSLFMINVVGFVFMPGGG
                     SEALTIKTFLPISKNLDTSLEAVREIEKLVLELPEDELESVYSRVGIEDNSIFDPRPG
                     DGTNKATVYVDLTSVSDRKRSAYQIEDLLREKIDEGIKSGKINKELVYSIEVKESGPP
                     VGKPVNIEIRGEDFATIEKIIAEYTSALEQYDGVFDITNDLEAGKSEIRYTIDDAKAA
                     RTNVNVAQLGSALNASYAGAVATNVKIDDEAIDVRVRFDETFRRSRESLDQVMVSNGK
                     GGLIPLQEVTKMKITPGYSQINRLNYKRLVQIQANIDLKKTTAVRVNTKLAEQFKDIK
                     DRYPGYDISYGGEQEDTAKSMSELGRLFLISILFIYIVLAAFFQSLLTPLVVMSAIPF
                     AMVGIVFAVYTHGQPLSFNGMIAFFSLAGGLVSSTVTLVEFINIKRAEGLKTRDAIAE
                     AGMLRIRPIILTTMTTVLGLVPSIYSGIKFFGLFSFGDRNYFVQPLALTYGYGLIFAT
                     VITLVLVPCFYHIAEDAKHGLAYILGLFGISMKKELY"
     gene            complement(2762..3751)
                     /locus_tag="P5123_06700"
     CDS             complement(2762..3751)
                     /locus_tag="P5123_06700"
                     /inference="COORDINATES: protein motif:HMM:NF024996.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015288 - porin activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="porin"
                     /protein_id="HRX15984.1"
                     /translation="MKKMYLVLMAVLALFFSANMFAANLYNADGTVVDLYSRLYAIYQ
                     QADQGEITGDCSRLGLQTSTKVNNDLSVFARAEFRYDASQREKETVFNDLRNTYVGVD
                     SSFGKVTVGNFDSVYYQAVSSKLDIYENAGFIALADGSTASRADSVSYSSPDLMGVKI
                     HGQIRHYGQDQTVSGDEKIMFQGGVTFDMNALSLGIAAVIADDEAEAVFVTETDATGE
                     ETTTATAKYSENIYGFSASYTLFEALTLIAMAEYQKDAGEADMNISGGVSYSYGMGDV
                     YGSLGQDCNEEMYYALGANWKFSKPMRIFVEYAGGDAVNPDNNIFTAGLRYDF"
     gene            complement(4089..4916)
                     /locus_tag="P5123_06705"
     CDS             complement(4089..4916)
                     /locus_tag="P5123_06705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011699728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PstS family phosphate ABC transporter
                     substrate-binding protein"
                     /protein_id="HRX15985.1"
                     /translation="MKTFKKILYPFFLLTIVFALFASCSKETGKTVVVKGSTTVLPIT
                     QLAAEEFRKETGIAVSIEGSGSGNGVKAIIDGTTDIGNSSRAMKPKELDEAKAKGVNV
                     KEIIVAYDMIIPVVHPENGVTDLTLDQLKGIYDGSITNWSQVGGADGNIAVISRDTSS
                     GTYEVWSEKVLKKNDVTAGALLQASNGAVLAAVAGNPRAIGYIGYGYLNNSVKALSVG
                     GIVGTVETGRSGEFPISRPLYKYVNADKVSENTQKFLDFLLSPKGQKLVEEAGFLPL"
     gene            5263..6015
                     /gene="pstB"
                     /locus_tag="P5123_06710"
     CDS             5263..6015
                     /gene="pstB"
                     /locus_tag="P5123_06710"
                     /EC_number="7.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011962535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009898 - cytoplasmic side of plasma
                     membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015415 - ATPase-coupled phosphate ion
                     transmembrane transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter ATP-binding protein
                     PstB"
                     /protein_id="HRX15986.1"
                     /translation="MKKIESKALNVYYGDTRAVKDISLQVEKNTVLTLIGPSGCGKST
                     FLRCINRMNDIIPGTRIEGELLIDGEPVYQNKYDVTTLRRKVGMVFQKSNPFPKTIFE
                     NIAYGLRINGIKDKYEIEKKVESSLKATALWDEVKDRLHQSAIGFSGGQQQRLCIART
                     IAIQPEIILMDEPASALDPISTQKIEELIHELKNDYTIIIVTHNMQQAARVSDYTAFF
                     YMGDLVELNKTEKIFTKPDLEMTEKYITGKFG"
     gene            complement(6134..6403)
                     /locus_tag="P5123_06715"
     CDS             complement(6134..6403)
                     /locus_tag="P5123_06715"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRX15987.1"
                     /translation="MLTRSVFIFSIFFFSVTSRSTTSTPCRVLSSLIGLMLTSYVLPL
                     ISSSSKWDGFAWARDCSIRRFRVRLRITSKKRSPLTFILVTSMHL"
     gene            6405..7049
                     /locus_tag="P5123_06720"
     CDS             6405..7049
                     /locus_tag="P5123_06720"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HRX15988.1"
                     /translation="MSHELKTPVTIIKGYLETIALNLNNTEQCSGYIRKAIENADRQT
                     SIINDIIKLNMIETSNDFANEKIIIDDLLEGCVNILGQKAQLRDIQLNKKLEAMGEGV
                     VGNKFLAEEIFFNLIDNAINYNLPGGEVNIESSISNNNQLTVKINDTGIGIPKESIDR
                     IFERFYRVDKGRSRESGGTGLGLSIVKHAATLLGWKITVRSDSKGSSFRVIIYL"
     gene            7340..9058
                     /locus_tag="P5123_06725"
     CDS             7340..9058
                     /locus_tag="P5123_06725"
                     /inference="COORDINATES: protein motif:HMM:NF019874.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein"
                     /protein_id="HRX15989.1"
                     /translation="MITCWKHFSIRKKIVITCLTVIFIFIAVILGLFLPYFKTYVISN
                     RKTHLNEMSSLAVATCEAYYYEHTLGRISKEEAFSKSLYHVNKFRYGKDESETFFIIS
                     SNGTGLSFPYREDLVGLNLRHISDPDGTKYYEKIIETALLQGNGFITYKTQYKSSVSN
                     IVPYLVYFTYYPNYDIIIGSGVYLHDIDREIHKIYIATVGITAIVSIIAALILCLLSG
                     TISKPVNTLVKTMASCDLNTRLPVQSGDEIGLLAGEFNSFNSRIKEIIVSVKNSMTEL
                     ATSSEELQSISENFSQKAADQNSHSKEISQNIDAISEKMSSVASEIDIEFVRLNSLMS
                     RMQNLSSLINSMNDTAHDASRQITKITAKASEGSRSLAFMTESIRAIENRSGELFRIL
                     DMINGISDQINLLSLNAAIEAARAGSMGSGFAVVSDEISKLAEETSGSLKTIGTLITD
                     NDRELKNGVDYISDSVRTINEVILGISEIGNTIKQMARKADEQTGSKEGVQREISEIK
                     EMSQNIRTQSRVQMQSIGEIHDIIHNINRGTSFITAGSEELASSSKEVSHMAERLQQL
                     IGGFKV"
     gene            complement(9160..10014)
                     /gene="pstA"
                     /locus_tag="P5123_06730"
     CDS             complement(9160..10014)
                     /gene="pstA"
                     /locus_tag="P5123_06730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014958594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0015415 - ATPase-coupled phosphate ion
                     transmembrane transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter permease PstA"
                     /protein_id="HRX15990.1"
                     /translation="MKRKADIIQLVTFVVIKLLSFLIIFFLIFMLGFIVKSGFSALSI
                     DFFTKMPENQMRGGGIMPAIAGTFLLMLGSAVVSIPVGIITAVYLNEYAKNPRVVKII
                     RLGINNLAGVPSVVFGLFGLSLFVIFLDFGTSILAGSLTLGVLSLPIIIRSTEEALLT
                     VPGTYREASLSLGATRWQTIYRIVLPNALPGILTGVMLSLGRSAGETAPIMFTAAAFF
                     APDLPRSVYSEVMALPYHIYVLATAGLHIQETRHLQYGTAIVLILLVLLLNTTGLVLR
                     YRLRRRLR"
     gene            complement(10027..10914)
                     /gene="pstC"
                     /locus_tag="P5123_06735"
     CDS             complement(10027..10914)
                     /gene="pstC"
                     /locus_tag="P5123_06735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012610563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0015415 - ATPase-coupled phosphate ion
                     transmembrane transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter permease subunit PstC"
                     /protein_id="HRX15991.1"
                     /translation="MKVHKSIVYDYLVEKLFFIIGLSCLVILFLIMFFLFKEGLPLLN
                     KVRISDFIFGMEWYPTSDNPRFGVFPLISASVAVTLIASLMAVPLSIAIAVYMAELAS
                     PITREIIKPVIEIIASIPSVVIGFFGMVVVAPFLQSYFDLDTGLNLFNAALMLGFMAV
                     PTIASISEDAISSVPVSLKEASYALGANRCQTIIFVTVPAALSGIWTAIILGVSRVIG
                     ETMVVLMVAGGAAMMPGSLFSPVRPLTSNIAAEMAETPVGSTHYHALFAIGILLFIIT
                     FVFNIIADYLSNKYKFKHN"
BASE COUNT         3695 a         2333 c         2130 g         3058 t
ORIGIN      
        1 gtcttacgta tgaaagggca atataccggg gtcgaagagg ttaagaagca ggttatacgc
       61 gctaatgacg gcggttttga aactaccctg atggatatag ccaaagtaag tgacacattt
      121 gaagaacccg aaataatcga acgaattaat ggcaaaaacg ctatagttct ggaagttttc
      181 aagaaacgaa gcatggacga gataactctg gctacaaata ttaaagaaaa cattgactcg
      241 ttcgatagtc ctttccccga cgatgtgaat attcttattt tcaatgattc aagtgaattt
      301 acagagaaga ctatcgacac ggtaataaac agcgccgtaa ccggtttctt tttatgcgcg
      361 cttgttcttc taattttcct agggccccgc atggcttcac ttgtaacatc cacaattcca
      421 ctggtttttc ttgtggcatt tatctttatg tcaatactcg atgttaacct taacgttata
      481 agtctttttg gtatgattat ggtacttgga atgatagtcg attttggtat agtcatcacc
      541 gaaaactgtc acaggtatat cgaaatgggg ctcgaaaagg gtgaagcaat tcttacaggt
      601 gtttgtgaaa tttttaaacc ggtaaccgtt tcatttatct gtatatcggc ctcatttgtt
      661 ccgcttcttg tactaacagg ccttatggga aaatttataa aaaatattcc gcttatgatc
      721 ctgatatgtc tctccgcctc ttggatagtc gcgctcttct ttatgccaac atttctaaag
      781 atgttttcga aagaagaaga gaaggtgcac aaagagcttg aaaccgaaga tggtgaacat
      841 ctcgagaagg gctttatggg aaggtttcag tttgcatata taggatttat tcgtcaggct
      901 ctcaggtttc gctatataac agtcgccata cttgttgcgg tactaggcgg atctctgttt
      961 atgataaatg tcgtcgggtt tgtctttatg ccgggcggcg gctccgaagc cttgacaatt
     1021 aagactttcc tgcctatatc caaaaacctg gacacctcac ttgaagcggt tcgcgaaatt
     1081 gaaaaactgg tcttggaact tcctgaggac gaacttgaaa gcgtctattc gcgcgtaggt
     1141 attgaagaca acagcatatt tgaccctcgc cccggagacg gtacaaacaa ggctactgta
     1201 tatgtcgatc tcacatccgt cagcgatcgc aagcgcagtg cttaccagat agaagatctg
     1261 ctgcgtgaaa aaattgacga aggcattaaa tccggcaaaa tcaataaaga acttgtttac
     1321 agcatagagg ttaaggaatc cgggccgcca gttggaaaac cagtaaatat cgagatacgc
     1381 ggagaagact ttgctacaat agaaaaaatc atcgccgagt acacatcggc tcttgagcag
     1441 tatgacggag tctttgacat taccaatgac cttgaggcgg gaaaatcaga gataaggtat
     1501 acaatcgatg atgccaaagc cgctcgtaca aatgttaacg ttgcccagct gggcagcgct
     1561 cttaacgcct cgtacgccgg agcagttgcc acaaatgtca aaattgacga tgaagcgata
     1621 gatgtaagag tacgctttga cgagacgttc agacgttcgc gtgaaagtct tgaccaggta
     1681 atggtctcta acggaaaagg aggcctgatt ccgctgcagg aagtaacaaa gatgaaaata
     1741 acaccgggtt actctcagat taatcgtctt aattacaaac gtcttgtaca gattcaggct
     1801 aatattgatc tgaaaaaaac aaccgctgtt agagtcaata ccaaacttgc ggaacagttc
     1861 aaagatatta aagaccgcta tcccggatac gacatttcgt atggaggaga gcaggaagat
     1921 acagccaaaa gcatgagcga acttggaaga ctgtttctta tttccattct ttttatctat
     1981 atcgtgcttg cagctttttt ccagtcactg ctgacaccgc ttgtcgttat gagcgctatt
     2041 cccttcgcga tggttggaat agtatttgca gtttatactc atggtcaacc attatcattt
     2101 aatggtatga tcgccttctt ctctctggcg ggaggacttg taagcagtac agtgacactt
     2161 gtcgagttta taaatattaa aagagcggaa ggccttaaaa ccagagacgc cattgcagag
     2221 gcagggatgc ttcgaatacg tccgattatt ttaactacaa tgacaaccgt attgggactt
     2281 gttccttcta tttacagcgg aataaaattt ttcgggctgt tcagtttcgg tgaccgtaac
     2341 tattttgttc agccactggc ccttacctac ggatacggtc ttatcttcgc gacggtgatc
     2401 acgctggtac tcgtaccatg tttttatcat attgccgagg acgcgaaaca cggcctagca
     2461 tatattttag ggctgttcgg tatcagtatg aaaaaagagc tctattgatt tgtaaatttg
     2521 ggcgccgctt cttttcgaaa cggtgcccta tttttaccct gccttttcca tcccaaccgc
     2581 aaacaggcgt tttttattta aaatagagca gccccaatta ttgtaaacga gtatccgtca
     2641 tcgaccggat accaagtaac aggaacatca agagtaaaag atttaaaccg ggaaggcacc
     2701 gaataactat cggtgccttt atttatatac taacagattg accaagaatc aagaagaact
     2761 gttaaaaatc gtaacgcaat ccggctgtaa atatattatt atccgggtta accgcatcac
     2821 caccagcata ctctacaaaa atacgcatag gtttactgaa cttccagtta gccccaagag
     2881 catagtacat ctcttcatta caatcctgcc ccaatgagcc atatacgtct cccattccat
     2941 agctgtaact gacaccccct gatatattca tatctgcttc accagcatct ttctgatact
     3001 ctgccatagc aatcaaggtt agggcttcaa ataatgtgta cgatgccgaa aatccataga
     3061 tattttcaga atactttgct gttgccgttg ttgtctcttc gcctgtggca tctgtttctg
     3121 taacgaatac agcctctgcc tcgtcatcgg caattacagc cgcaatgcca agtgaaagag
     3181 cgttcatatc aaatgtcacc cctccctgga acataatctt ttcgtctcca gatactgtct
     3241 gatcctgacc gtaatggcgt atctgcccat gaattttaac acccatcaaa tcaggagagc
     3301 tataagaaac actatcggca cgcgaagcag ttgatccatc agcaagtgca ataaaaccgg
     3361 cattttcgta tatatcaagt tttgacgaca cagcctgata atagacagaa tcaaaattac
     3421 ccacagttac tttaccgaaa ctactgtcaa cacccacata ggtgttgcga agatcgttaa
     3481 acaccgtctc tttctcgcgt tggcttgcat cgtaacgaaa ttcggctctg gcaaaaacag
     3541 acaaatcgtt atttaccttc gttgaggtct gaagtcccaa acgagaacaa tcgcctgtaa
     3601 tctcgccctg atcggcctgt tgatatatgg cgtaaagcct gctgtaaaga tccaccacgg
     3661 taccatccgc gttataaagg ttcgctgcaa acatattcgc cgaaaaaaac agcgctaaaa
     3721 cggccattaa tactaaatac atttttttca tttaacttta tctctccttt acacttattg
     3781 tgcattatcg agataccttc acttcctgat aatgagcaga gggtaaaagt atcacttttt
     3841 tgctactcgg tagtcgattt aaattttgcc gatttttacg gctgccgagt acactaacaa
     3901 aactaatctt atatatttta ttatgacaca agatcgtatt aaaatataat actcgtttgc
     3961 tttatttcat ttttttgtct aacaaaacca ttaataattc acgaaaaaca gttacttttt
     4021 tacatgttta taatatagac aatattaagc agaacggatg ttccgctctg ctcaaagata
     4081 tgtctctttt atagaggtaa aaatccggct tcttcgacca atttttgtcc ttttgggcta
     4141 agaagaaaat caagaaactt ctgtgtgttt tcacttacct tatcagcgtt cacatattta
     4201 taaagcggcc ttgaaatagg aaattctcct gatcggccgg tctcgactgt tccaacgata
     4261 cccccaacac taagagcttt aacagaatta ttaagatatc cgtaaccgat ataaccaatc
     4321 gcacgaggat taccagctac cgctgccaaa actgctccat tagatgcctg taagagtgct
     4381 cctgctgtta cgtcattttt tttaagaacc ttctcactcc agacctcata cgtgcctgag
     4441 ctggtatctc gcgaaataac cgcaatattt ccgtcggctc cgccaacctg actccaattt
     4501 gtaatagaac cgtcatagat gcctttaagt tgatcaagcg ttagatcggt aacaccattt
     4561 tcgggatgta caaccggtat gatcatgtcg taagcgacaa tgatctcctt aacgttaact
     4621 cccttggctt tagcctcgtc caactcctta ggcttcatgg ctctagatga attacctata
     4681 tcagttgttc catcgataat ggccttaacc ccgtttccgg atccacttcc ttcaatagaa
     4741 acagcaatac ctgtctcttt acgaaattcc tcggctgcaa gctgggtaat aggcaatacg
     4801 gttgttgaac cttttactac aactgttttc cctgtctctt tggagcaaga tgcgaacagc
     4861 gcgaaaacaa tggttaataa gaaaaaagga tataagattt ttttaaatgt cttcattaat
     4921 aaagaccccc tgaattatgt ttaaaagaac aataaataaa tcaggtaaag attttataaa
     4981 gactctgtat ggaaaaaatc aatttaaaca actctatgaa gattacaaag aaaatgctca
     5041 ctaacgttga agcccatcaa acaacgaata gctgtaaaaa ttaacatttt ctttataatt
     5101 tcattataaa acccacacct ttttttaata cctctcatta taacgactta tcaatctgat
     5161 taagtgaaac taccagttta cttaaaaatt gataagcaac attcgcaata aatgagatca
     5221 gggtgtcaaa tcagactata cgatcctgac aaaggcagaa taatgaaaaa aatcgagtca
     5281 aaagcactta acgtatatta cggcgacaca agagccgtta aggacatctc tcttcaggta
     5341 gaaaaaaaca cagttcttac cctgataggc ccttctggct gcggcaaatc gaccttctta
     5401 cgttgtatta accgtatgaa cgatattata ccgggcacac gaatagaggg agaacttctt
     5461 atagacggag aaccggtgta tcagaacaaa tatgatgtta caacacttcg ccgcaaggtg
     5521 ggaatggttt tccaaaagtc gaatcctttt cccaaaacaa tattcgaaaa catcgcgtac
     5581 ggattacgta taaacggaat aaaagacaag tacgagattg aaaagaaggt ggaaagctca
     5641 ttaaaggcga cagctctctg ggatgaagtt aaagaccgac ttcatcaatc tgccatcggt
     5701 ttctcaggcg ggcagcagca gaggctttgc attgcacgaa caattgcgat acagcccgaa
     5761 attattctaa tggacgaacc ggcatcggct ctagacccaa tatcgacaca aaaaattgag
     5821 gagctgatac acgagcttaa aaacgactac acaataatca tcgtaacaca caacatgcag
     5881 caagcagctc gtgtcagtga ctatacggcc ttcttttata tgggtgatct ggttgaactt
     5941 aacaaaacgg aaaagatatt taccaaacca gatcttgaaa tgaccgaaaa atatataacc
     6001 ggcaaatttg gctgataaat tacagacaaa aacattaaat gcaaattata aggagaaaca
     6061 gatgataaca catctctgat ggaataactg ttatcaataa agagaaaaaa atagtaattg
     6121 ttaacaaggc attttacaaa tgcatagacg taacaagaat aaaagttagc ggacttcttt
     6181 tctttgaagt tatacgcaac cgtactctaa atctgcgtat tgaacagtct cttgcccagg
     6241 caaaaccatc ccattttgaa gaagagctta tcaatggaag aacatatgat gtaagcatca
     6301 atccaataag cgaagagaga accctgcaag gtgtacttgt tgtactacga gatgtaaccg
     6361 aaaagaaaaa aatagaaaag ataaaaaccg atcttgtaag caatatgtca cacgaactta
     6421 aaaccccggt aacaataatt aaaggttacc ttgaaaccat tgctttaaac cttaacaata
     6481 cagaacagtg ttccggatac ataagaaaag ctatcgaaaa tgctgaccga cagacatcta
     6541 taatcaacga tataataaaa cttaacatga ttgaaacctc taacgatttt gccaacgaaa
     6601 agataattat tgacgatcta cttgaaggtt gtgtaaacat actaggacaa aaggctcaac
     6661 tgcgtgatat tcaattaaat aaaaaactgg aagccatggg agaaggagtt gttggtaata
     6721 agtttctggc cgaagaaata ttctttaacc tgatagacaa cgcgattaac tataaccttc
     6781 ccggaggaga agtaaacata gaaagcagta tctcaaacaa caatcaactg acagtcaaga
     6841 taaacgatac aggaattggc atacccaaag agtctattga ccgtatattc gaaagatttt
     6901 acagagtaga taaggggcgc tctcgtgaat ccggcggaac cggtcttggt ctttcgatag
     6961 tcaaacacgc agcgaccctg cttggttgga aaataacagt cagatctgat tctaaaggaa
     7021 gttcttttag agtaattatt tatttatagc tatcatcaaa cggccatctt tagaaaaata
     7081 tcctttcaca cgccaagctc tttctcttga tctttacttc tatttaaggt ttggtgattg
     7141 ccctctttcg tgcattagac taaatttaat tggcatcacc ataagatata actacaaaca
     7201 taagtccgaa atacatggca aaaaaccatt acctaacatt ttctttatat tattcataca
     7261 taatagtgct ctgcttattc taaagtagtt tcgttcaaaa ttcgagctct gctttacaca
     7321 ataatcagag gtacggtcta tgatcacttg ctggaaacat ttctcgataa gaaaaaaaat
     7381 agtaattaca tgtcttacag taatttttat ttttatagcg gtaatactgg gacttttttt
     7441 accatatttt aaaacttatg ttatatctaa ccgcaaaact catcttaacg agatgagctc
     7501 attagctgtc gcgacttgcg aagcatacta ctacgagcac acacttgggc gaatttcaaa
     7561 agaagaggca tttagcaaat cgctttacca tgttaacaag tttagatacg gcaaagacga
     7621 atcagaaacg ttttttataa tatcatctaa cggaacaggt ctctctttcc cctatcggga
     7681 agaccttgta ggacttaatc taagacacat aagtgaccca gacggtacaa agtactacga
     7741 aaaaattatt gaaacagctt tactgcaagg caatggattt attacctata aaactcagta
     7801 taaatccagt gtatcaaata ttgttcccta ccttgtctat ttcacctatt accctaatta
     7861 cgacattata attggctccg gtgtttacct gcatgatatt gaccgcgaaa tacataaaat
     7921 ctatatcgca acggtaggca ttacggctat tgtatccata attgcagcgt taatactatg
     7981 tctattgtcc ggcacaatat ctaaaccggt taacaccctt gtaaaaacaa tggcaagttg
     8041 tgatcttaat acacggcttc ctgttcaaag tggtgatgaa attgggctac ttgccggtga
     8101 atttaacagt tttaattctc gaataaaaga gattatcgta tcagtaaaaa actctatgac
     8161 cgagttggct acttcatcgg aagaattaca atcgatttcc gaaaactttt ctcaaaaagc
     8221 agcagatcag aatagccact caaaagagat atcacaaaac atagacgcaa tttcagaaaa
     8281 aatgagttcg gtggcttcag aaattgatat tgaatttgta cggcttaact cattaatgag
     8341 ccgaatgcaa aatttatcat cactaataaa ctccatgaac gataccgctc acgacgcgtc
     8401 cagacagatc acaaaaataa cagcaaaggc ctccgagggc agtagatctc ttgcgtttat
     8461 gaccgaaagc attcgcgcga tcgaaaaccg ctcaggagaa ctatttagaa tactcgacat
     8521 gataaacgga atatcagatc aaattaacct gctatctctt aacgcagcaa ttgaggctgc
     8581 cagagcaggc agcatgggaa gcggctttgc tgtagtatct gatgagatat ctaaacttgc
     8641 cgaagagact tcgggaagtc taaagacaat tggtacactg atcaccgaca acgacagaga
     8701 gcttaaaaac ggtgtagact atatttccga ttcggtcaga acaataaatg aagttatatt
     8761 aggtattagc gaaattggta acacaataaa acagatggct aggaaggctg acgagcagac
     8821 aggctctaag gagggagtac aacgcgagat tagcgaaata aaagagatgt cacaaaacat
     8881 tagaacacaa agccgggttc agatgcagtc tatcggagaa attcatgata ttatacataa
     8941 tattaacaga ggcacctctt tcataacggc aggctcggaa gagttggcct caagctctaa
     9001 agaagtttca cacatggcgg aaaggctaca acagctgatt ggcggattca aggtttaggc
     9061 aacctcaact ggttgccttt taagcaagat gaataaatga atcattctgc aaatcataaa
     9121 atataataaa gaataaagaa caaagaaaat aaactcccat taacgaagac gtctgcgcaa
     9181 cctgtacctg agcacaagac ctgtggtgtt caaaagcaga acaagcagaa tcagcacaat
     9241 tgccgtaccg tactgcaggt gtcgtgtctc ttgaatatga agcccagcag tagccaaaac
     9301 atagatatga taaggcaagg ccataacctc actataaaca gaacgtggca aatctggagc
     9361 aaaaaaagct gcagctgtaa acatgatcgg tgccgtctct ccggccgaac gtccaagtga
     9421 taacattacg cctgttaaaa tacctggcaa cgcgttaggc aaaacaatac ggtaaattgt
     9481 ctgccacctt gttgctccta aagataaaga tgcttcacgg taagtcccag gtacggtcaa
     9541 aagcgcctct tcggtcgagc ggataattat cggaagactt aacacaccaa gtgtaagcga
     9601 gccagcaaga atggatgttc cgaaatcaag aaaaattaca aaaagactca gaccaaaaag
     9661 gccaaaaacc accgaaggca cacccgccag attatttatt ccaagcctta ttatttttac
     9721 aacacgggga tttttggcat actcgttaag ataaaccgcg gttattattc caacaggtat
     9781 tgatacaacc gctgacccga gcataagcag aaaggttcct gctattgccg gcattatgcc
     9841 tcccccacgc atctggttct ccggcatctt tgtaaagaaa tctatactca aagcagaaaa
     9901 tccactcttg actataaacc ccaacatgaa tatcagaaaa aatattatta aaaacgaaag
     9961 caacttaata acaacaaagg ttacaagttg aattatatca gcctttcttt tcatttaacc
    10021 tccggactag ttatgcttga acttgtactt atttgaaagg taatcggcaa ttatattaaa
    10081 aacaaacgtg atgataaaaa gcaaaattcc tatagcgaat agtgcatgat agtgggtaga
    10141 tccaacagga gtctcagcca tctctgctgc aatattcgaa gtaagcggcc ttacaggtga
    10201 aaatagcgaa ccaggcatca tagcagcgcc tccggctacc atcaaaacaa ccattgtttc
    10261 accaataaca cgtgaaactc ccagaattat tgctgtccag ataccggata atgcagcggg
    10321 cactgtaaca aaaataatgg tctggcagcg gttggctcca agagcatacg aagcctcttt
    10381 taacgataca ggaactgaag atatcgcgtc ttcgctgata ctcgcaattg ttggcacagc
    10441 cataaatccc agcatcagag ctgcgttaaa aaggttaagt ccagtatcca gatcaaaata
    10501 gctctgcaaa aatggcgcga caacaaccat gccaaaaaat cctattacta cagaggggat
    10561 cgaggcaata atttcgataa caggttttat tatttcgcgg gtaataggtg aagcaagctc
    10621 ggccatgtaa actgcgattg cgatacttaa cggaacggcc atcaatgatg ctattaatgt
    10681 tactgctacc gatgcagaaa taaggggaaa aactccaaaa cggggattgt cgcttgtcgg
    10741 gtaccactcc ataccaaata tgaaatcgct tattctaacc ttatttagca gaggcaatcc
    10801 ctctttaaat agaaaaaaca ttataagaaa aaggattaca agacaactta gacctattat
    10861 gaagaaaagt ttttcaacca gatagtcata aacaatactt ttatgaactt tcatttatac
    10921 aacctgccgc tatttcatta ttaaaaacac cagtttttgt ttgcaactaa caaaaaccag
    10981 tgagttgtta gaacttagcg gcagtttgta aattttttat aaagatttta taagtattta
    11041 aacaatagag atgatgtttt atataaagat ctattttgca gaattgatta tttccagtgt
    11101 tttatcaaga gcgtcctctt tcgaaccgat ctgaacatca ggaacggttc ccacctcttc
    11161 attacaggtt ccgtctggat ttaaagacat gaaattagag aaacgataaa taagtt
//
LOCUS       DAOTGZ010000078        10706 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_3064
            NODE_7329_length_10706_cov_4.847057, whole genome shotgun sequence.
ACCESSION   DAOTGZ010000078 DAOTGZ010000000
VERSION     DAOTGZ010000078.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868088
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 10706)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10706)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:22:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,785
            CDSs (total)                      :: 2,751
            Genes (coding)                    :: 2,732
            CDSs (with protein)               :: 2,732
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 12 of 19
            Pseudo Genes (internal stop)      :: 5 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10706
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7329_length_10706_cov_4.847057"
                     /isolate="AMR_MDS_3064"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1148)
                     /locus_tag="P5123_06740"
     CDS             complement(<1..1148)
                     /locus_tag="P5123_06740"
                     /inference="COORDINATES: protein motif:HMM:NF015382.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carcinine hydrolase/isopenicillin-N
                     N-acyltransferase family protein"
                     /protein_id="HRX15992.1"
                     /translation="MLIKPKQHIEQIIARVSNKLKFDALVVGQISDDLSMFRKVDVSG
                     SFYKLGIFFGHCAIELGYGFPKLKKKNGDLNLQIIDMYKDCYPQYLKQLQGFAHVYGA
                     SIEAMDLTMMEPFFARMGYEGYRIKGSAKSGFKNMCTVIGATGSNGKTYIGRNLDTFN
                     ITGFLVNSKMEGVYKTVNTSGEAFCNYVVDGINEKGLFVGEMSIPDPRYKKNLKLRYP
                     DVPSVYCAHMLRIILDTCANVNEAATLLQKVPVWFTNDLWHIYLADSGGSFCIAEWDE
                     NGKIVLQHFDKKLIATNTPIMEGDEKLNKCPRYSLANNLINNKDIDSHESIYHLMKAL
                     SYRYDNTYLKKLVFKETELRAMKYLRTVWTSTYDLDKLSMIIRYHENG"
     assembly_gap    1208..1217
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            1376..1714
                     /locus_tag="P5123_06745"
     CDS             1376..1714
                     /locus_tag="P5123_06745"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRX15993.1"
                     /translation="MIKRVLSLFILISFIMIIIACASTPQPVTQKEKEEVFKSELVMF
                     YTRCHEESKRKKTSKSDDDFHNIFKTNTVNLFNSKISKTKRSRSLKKMNALCSEELTR
                     WLDRASKIDY"
     gene            complement(1851..2912)
                     /locus_tag="P5123_06750"
     CDS             complement(1851..2912)
                     /locus_tag="P5123_06750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006423936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRX15994.1"
                     /translation="MSDYYGFPEYVSVAQRKKKAKEALEKIKKKNSQVKPVVIDGTKI
                     AKTWWGIAWIKNLECYADYSNRIGRGRSYVRHGSVLDLQIEKGLVRALVKGSRSKPYK
                     IDIKITPLAKARWKEMILVCEGKLDSLQELLSGKFPTALADLFTIKNKGLFPSPKQIE
                     FDCSCPDWASMCKHVAAVLYGVGARLDEDPSLFFLLRGVNVDDLISKAVIAKSDKMLK
                     QASAAGRRVIKDANILETFGIDSDELSVEKTSPNLIKKTTSKKETAKKAVSKKVTSKK
                     AAAKKSGSKKTTAKKAAAKKSGSKKSGSKKSGSKKTTAKKAAAKKSASKKVTSRKAVS
                     KKIVSKSAVSKKTSSKRSS"
     gene            complement(3010..5652)
                     /locus_tag="P5123_06755"
     CDS             complement(3010..5652)
                     /locus_tag="P5123_06755"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009171139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HRX15995.1"
                     /translation="MKRKLIVIIEPEAGYIFDWDEESEQPDSFCEELFLRYKKDPLAA
                     LLFLGFQNNNSEWSESLRYLWSIASSFVHLLTKMEELESRREKVLVDEDAETFESLIK
                     SAPFICGSENLSIEWLSNLWINLNHTFCTQIKAFKGTVSDYLHQNNPALHLPGRVYFH
                     LVENKSGEEPFAFLATYTTYSSQKAKSRHLPLKNALEEYGDNTRKMLELLSTVNKASE
                     KSSLIAEVVQSGEIFYPLNFSAVDAYTFLKEVSLYEESGIICRIPDWYRKKQNSLSVA
                     ITVGDKKKSYFSQDTLLSFDISISLGADTISVKQAKQLLSEAEGLTMIKNRWVEVDHQ
                     RLKEVIKLYEKAESEYVNSGITLSQAIRIQLNEGKLFTEKSDSDEIEVNNGTWLTEIM
                     AGLTRPESLPDIDAGNNFNAVLRGYQLKGLNWLHYLNLIGLGACLADDMGLGKTIQVI
                     AHLNHLSLKQKKHKSLIVIPASLIGNWVNEIKCFAPSLRYHVVHPSNALASSIDDVYA
                     NKNGGVYITTYAILVRNKWFSQITWDYLILDEAQAIKNPNTKQAKAVKVVPASYRLAL
                     TGTPVENSLMDLWSIFDFINPGLLGTSKEFISFTKSLSDNPEGYTRLKKIISPFILRR
                     LKTDTKIISDLPEKIEMKTYSNLTPKQALLYQNLVKSLRNALDSTEDGISRKGLILAS
                     IMKFKQICNHPDQYTGQDNYSETDSGKFERLREICETIKAKRERVLVFTQFKEITEHL
                     SDYLARLFGHRGLVLHGGIVVKKRKDLVEAFQGREYVPYMILSITAGGTGLNLTAANH
                     VIHFDRWWNPAVENQATDRVFRIGQKKNVIVHKFITAGTIEEKIDLMIDDKTKLVNDI
                     LPEKQENWLTEMSNDEIMQLVSLV"
     gene            complement(5723..7246)
                     /locus_tag="P5123_06760"
     CDS             complement(5723..7246)
                     /locus_tag="P5123_06760"
                     /inference="COORDINATES: protein motif:HMM:NF012437.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009190 - cyclic nucleotide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0035556 - intracellular signal transduction
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate/guanylate cyclase domain-containing
                     protein"
                     /protein_id="HRX15996.1"
                     /translation="MDVKKKKSLRIVVMTMLAVSLVNPLSNVGAVVIIFLVGPLVFTP
                     ADWVASEQLFAMIRDMEWLNMVIPSVLVIIYLTPILIAMYRKLMGKPAKRFRPGRILD
                     VPLKISLIGISGWIVEEILFLSIWFKQPLIATSTLIVTSLEMLLAAIFSFVVSFFILE
                     MVFRRYVIPRVFPEGKLSKLAGNTNFSIRGRFFVYYITAALFPIYIFSLFVLSRIEES
                     DSYTGYIAMIVVILVSGAALTYLVSGYFREPLLLMKKAVLDIRKANYKIDLPVTSNDE
                     LGLLSENLNDLGRELLEKELMKDTFGKIVDPSVRDHLMNGNMNLGGEERTVTVLFTDI
                     RNYTGLTEKMDPETVVLWLNRYFDAVSEPITRYGGIVNKYIGDALMAVFGAPSEDKKH
                     ASNGVAAALAMIEAREKLNRQFASEGLPTINAGIGVHSGTVLAGNIGSSSRMEYTVIG
                     DTVNVAARVEKMTKELKLSLLITEMTREQLEADARFKKAALCKLRGRSEQVKLYKPV"
     gene            complement(7474..8571)
                     /locus_tag="P5123_06765"
     CDS             complement(7474..8571)
                     /locus_tag="P5123_06765"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF013884.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase"
                     /protein_id="HRX15997.1"
                     /translation="MKQRYYYLDNLKVFLTMLVIAHHVSITYGGSGDWYIIDPNHNEL
                     TSLILTLFTAVNQSFFMGLFFFISGFFTPGSYDKKGMASYLRDRLIRLGIPLVLFYTI
                     LNPLLVYILRVYYGEYNGSFVTFWFKNMPNYAGPGPLWFVQTLLIFSIVYAIVRMIGG
                     KSNKTTDFPSNGKLIVGAICAASVAFVIRIFLPADKALLSLKLGYFPLYVLLFVSGIV
                     ASRNNWLDKIDNSVTKRWFITSLVLMVVLPIVIIVSGNTDAFNGGFTIQSLMYCFWDP
                     FVCFGICFKLLQLFRTKINTAPTILKEAGRGAYVVYITHPFFCVGAAILLMPLDFAPI
                     IKFLLCSIIAILTAFISATLIVRVPVVNRIV"
     gene            complement(8568..9191)
                     /locus_tag="P5123_06770"
     CDS             complement(8568..9191)
                     /locus_tag="P5123_06770"
                     /inference="COORDINATES: protein motif:HMM:NF036520.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HRX15998.1"
                     /translation="MSNSKIRVPQQDRSKESKNSIIEAGLQLFSEKGYYKTNSKEIAK
                     RAGVSIGSFYMYFADKKALFMEVVELYNQKICDVLNDIENPGNGSINDGKTFLNYLIT
                     KLIEAHEVYPQLHQELTVMSASDPDIATLYNNARVEAISLAKKFLSSRIDNLRISDIE
                     TISVLIQTTLEEIVHIIVFSDVTISKEKLIDETTDMLYRYLYKDEKL"
     gene            9404..9964
                     /locus_tag="P5123_06775"
     CDS             9404..9964
                     /locus_tag="P5123_06775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015949437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HRX15999.1"
                     /translation="MSVLETISGSGMDRIPNIAFRGMSFLFAIWEIFASPEKLLKKFK
                     IKEGNTVVDYGCGPGSYIKAASTLAGQNGIVYAADVHELAIAAVNKKIERYGLTNVKT
                     AQVTDYKSEIPDNCADVVYALDMFHMVKTPNLFLKEIHRIIKKDGRLYIEDGHQSRKN
                     SKKKIESSKSWKIEEEYRGFLVCSPV"
     gene            complement(10281..10520)
                     /locus_tag="P5123_06780"
     CDS             complement(10281..10520)
                     /locus_tag="P5123_06780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007293883.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6290 family protein"
                     /protein_id="HRX16000.1"
                     /translation="MSRTVTLRLDDEIYKIIKQHANADNRPLSNYIETATLKYIENID
                     FVDDYEMDHILNDKELLDSLRRGNTDAKNRNGRFV"
BASE COUNT         3292 a         2171 c         1872 g         3361 t
ORIGIN      
        1 taaccgtttt cgtggtaacg aattatcatg cttagcttat ccagatcata ggtggaagtc
       61 cagacggttc taagatattt cattgctctt aattctgttt ctttaaatac cagcttttta
      121 agataagtat tatcataacg ataagagaga gccttcatta aatgatagat actctcatgc
      181 gaatcaatat ctttgttgtt aatcaaatta ttggctaatg aataacgcgg acacttgttt
      241 aatttttcgt ctccctccat aataggggta tttgtcgcga tcagcttttt atcaaaatgt
      301 tgcaaaacaa tttttccgtt ttcatcccac tccgcgatac aaaaacttcc accagaatcc
      361 gctaaataga tatgccacag atcgtttgta aaccagaccg gtaccttttg taaaagagtg
      421 gccgcttcat taacattggc gcatgtatca agaataattc taagcatatg cgcgcaatag
      481 actgaaggaa catctggata tcggagtttt aaattttttt tatatcttgg atcgggaata
      541 ctcatctccc cgacaaagag ccctttttca tttatgccat caaccacata attgcaaaaa
      601 gcctcgcccg aagtatttac agttttatac accccttcca ttttgctatt taccaaaaac
      661 ccggtaatat taaaggtatc aaggttgcgg cctatatatg tttttccgtt agaacctgtt
      721 gcgcctataa ctgtacacat gtttttaaaa ccgcttttag cacttccttt aatacgatat
      781 ccctcataac ccattcgggc aaaaaacggt tccatcatag taagatccat tgcttcaata
      841 gaagcgccat agacatgtgc aaatccttgc aactgtttca gatattgcgg ataacaatct
      901 ttatacatat caattatttg caggtttaaa tccccgttct tttttttaag ttttggaaaa
      961 ccgtacccaa gttctatggc gcagtgccca aaaaaaatgc caagtttgta aaaactgccg
     1021 gacacatcaa cttttctaaa cattgataaa tcatcactaa tctgaccaac aactaatgcg
     1081 tcgaatttaa gcttattaga aacccgtgcg attatctgtt cgatgtgctg ttttggtttt
     1141 attaacataa agagcctatt tggccggttc atgttcggcc agccgaaaca ttaccggatc
     1201 agttgttnnn nnnnnnnccg tgagattatc tgttcaatat tttgttttgg ttttattaaa
     1261 acttctttca ttaaatttac ccgacaatag tgtgaaaata ttagtatttg acaaaaaaat
     1321 atttgagtcc aaatcgttac tacgaaaaaa atattcatta ctgaaggagt ccaaaatgat
     1381 taaacgcgtc ctgtcacttt ttattctgat ttcatttatt atgatcatca tagcttgcgc
     1441 ttcgacaccc cagcccgtta cgcaaaaaga aaaagaagaa gtttttaaat ctgaactggt
     1501 catgttttat accagatgtc atgaagagtc taaaaggaaa aaaacctcca aaagcgatga
     1561 tgattttcat aacatattca aaaccaacac agttaatctt tttaacagca agatatcaaa
     1621 aacaaaaaga agtagatctt taaaaaagat gaatgcccta tgtagcgaag agctaacccg
     1681 atggcttgac agagcaagca aaattgatta ttaaacagac cgactgtttt aattttcaaa
     1741 tctctatctt ttgacctgaa tccggcttat ataacttaac ctgttctgtg tgccccctaa
     1801 gtttgcagag ggctgctttt ttagcagatc cagaaaacct accagctgcg tcaagacgat
     1861 cgttttgaag acgttttctt tgaaacagca ctctttgaaa cgattttctt agaaacggct
     1921 tttcttgaag taactttctt tgaagctgat ttctttgcag ctgctttctt tgcagttgtt
     1981 ttctttgaac ctgatttctt tgaacctgat ttctttgaac ctgatttctt tgcagctgct
     2041 ttctttgcag ttgttttctt tgaacctgat ttctttgcag ctgctttttt tgaagtaact
     2101 ttctttgaaa ctgctttctt tgcagtttct ttttttgaag tagtcttttt aatcaaattt
     2161 ggtgaggtct tttctaccga taactcatca gagtcgatcc caaaggtctc aagtatattt
     2221 gcatccttta ttacgcgtcg ccctgccgct gaagcctgct tcagcatctt gtctgactta
     2281 gcaattacgg cttttgatat aagatcatca acattaactc cccttaaaag aaaaaaaagc
     2341 gatggatctt catccaatct ggcaccgact ccatacaaaa cagccgcaac atgcttgcac
     2401 atggaggccc agtcgggaca agagcaatca aactcgattt gctttggcga cggaaagaga
     2461 cctttatttt ttatggtaaa tagatcagcc aaggctgtgg gaaacttacc cgaaagtagc
     2521 tcttgcaacg aatcaagttt accctcgcaa acaagaatca tctctttcca tcttgcttta
     2581 gcaagcggag taatcttgat atcaattttg taaggtttgg atctgctccc cttaacgagc
     2641 gcgcgaacca gccctttttc gatctggaga tctaagacag atccatgtct tacataactt
     2701 ctaccacgcc caatgcgatt tgagtaatca gcgtaacact caagattttt tatccaggca
     2761 atcccccacc atgttttagc gatttttgta ccatcgataa caacgggctt aacttgactg
     2821 ttcttttttt ttattttttc taaggcttct ttcgcttttt tctttctttg cgctacagaa
     2881 acatattctg gaaaaccata ataatctgac acggcagcac tcctaaagag ctgtattttt
     2941 ttagacagta aattggtgtt gtacaataac tttggtttta attcatatag attcgtttat
     3001 agctttgtat cagacaagag atactagttg cataatttca tcatttgaca tctctgttag
     3061 ccagttttcc tgtttttcag gaagtatatc gttaaccagt ttcgttttgt cgtcaatcat
     3121 aagatcgatt ttttcttcga ttgttccagc cgttataaat ttgtgaacaa taacattctt
     3181 cttctgtcct attctaaaaa ccctatcagt tgcctgattt tctacagccg ggttccacca
     3241 gcgatcaaaa tgtataacgt gatttgccgc ggtaagatta aggccagtac ctcccgccgt
     3301 gattgatagt atcatataag gtacatattc gcgcccctga aacgcttcta caagatcttt
     3361 tcgctttttt actacgatcc caccatgcaa cacaagcccc ctatgaccaa agagacgcgc
     3421 aagataatca gaaagatgtt cagtaatttc tttaaactga gtaaagacaa gcaccctctc
     3481 gcgtttagct tttatcgtct cacagatttc tcgcaaacgc tcaaatttgc cgctgtctgt
     3541 ttcgctatag ttatcctgtc ctgtatactg atctggatga ttacaaatct gtttaaactt
     3601 cataatcgaa gcgagaatta atcccttgcg actgattcca tcttcggtac tatcaagtgc
     3661 gtttcgaaga gacttaacaa gattctgata aagtagtgcc tgctttggag tgagattaga
     3721 atatgttttc atctcaattt tttctggtaa atcagatatg attttcgtgt ccgtttttaa
     3781 tcggcgcaaa ataaacgggc tgataatttt ttttagtcgt gtataccctt ctggattatc
     3841 actaagactt tttgtaaaac taataaattc ttttgaggtg ccaagcaatc ccggattaat
     3901 aaaatcaaag atcgaccata aatccattaa agagttctct acgggagttc cggtaagtgc
     3961 aagtcgataa gaagccggaa ctacttttac agctttagct tgtttggtat ttggattttt
     4021 tattgcctga gcctcatcta aaattagata atcccaggta atttgtgaaa accatttgtt
     4081 tcgcaccaat atagcatagg ttgtaatata tacaccaccg tttttattag catatacatc
     4141 atcaattgaa gaagccaagg catttgacgg gtgtacaaca tgatatcgca gacttggtgc
     4201 aaaacacttt atctcgttta cccagttacc tataagtgaa gctggaatga caatcaaaga
     4261 cttatgcttt ttttgtttaa gtgaaagatg atttaaatgt gcaataacct gtatcgtttt
     4321 tccgagtccc atgtcatctg caagacaagc gccaagacca atcaaattta gatagtgcag
     4381 ccagtttaaa ccttttaact gatatcctcg aagaactgcg ttaaagttat ttcctgcatc
     4441 gatatccggc aatgactccg gacgagtaag cccggccatt atctctgtta gccatgtacc
     4501 gttatttact tcaatctcgt cagagtcgct tttttcggta aaaagctttc cttcgttaag
     4561 ctgtattctt attgcctggg ataaagttat accactgttt acatattcag attctgcttt
     4621 ttcatatagt ttaatgactt ctttaagcct ctgatgatca acttcgaccc aacgattttt
     4681 tatcatcgtt aaaccttcag cttcgcttaa caattgcttt gcctgtttta cagaaattgt
     4741 atccgcaccc aaagaaatag aaatatcaaa actgagcagg gtgtcttgcg agaagtagct
     4801 cttttttttg tcacctaccg tgatcgctac agaaagcgaa ttttgttttt tacgatacca
     4861 atccggtata cgacatataa tgccggactc ttcatagaga ctaacttctt ttaaaaaagt
     4921 ataagcatca acggccgaaa aatttagagg ataaaatatt tctcctgatt gtacaacttc
     4981 tgcaattaga gagctttttt ctgatgcctt atttacagtc gaaagcagtt caagcatttt
     5041 acgtgtatta tctccatact cttcaagagc atttttaaga ggaagatgtc ggctctttgc
     5101 cttttgtgaa gaataggttg tataggttgc caaaaaagca aaaggttcct ctcccgattt
     5161 attttctaca agatggaaat agactctacc tggaagatga agtgccggat tattttgatg
     5221 cagataatct gataccgtcc ccttgaatgc tttaatctga gtgcaaaagg tgtgattaag
     5281 atttatccaa agattactca accattctat agagagattt tcagatccgc aaataaaagg
     5341 tgcggactta attaggcttt caaaggtttc ggcatcttca tctactaaga ccttctctct
     5401 tctgctttca agctcttcca tcttcgttaa caaatgaaca aatgaggatg caatactcca
     5461 cagataacgt aatgattcgg accactcact attattattc tgaaacccaa gaaataaaag
     5521 ggctgccaaa gggtcctttt tatatctcaa aaacagctct tcacaaaaac tgtccggctg
     5581 ttctgactct tcatcccaat caaaaatata accagcttct ggttcaataa tcacgatcag
     5641 ttttcttttc atattgcttt tcaccacata gattatgtta gaaggctaga tttaataata
     5701 atagacaaca agtaaaaaaa atttataccg gcttataaag cttaacctgc tctgatcgtc
     5761 ctctcagttt gcagagtgct gcttttttaa agcgggcatc tgcctcaagt tgctctcttg
     5821 tcatctctgt tattaacaga gaaagtttca gctcttttgt catcttttct actcgcgccg
     5881 caacgttaac ggtatctcca attactgtgt actccatgcg gcttgaagag cctatattgc
     5941 cagccaaaac agttcccgag tgaacaccaa tacccgcatt aattgttggt aatccttcgg
     6001 atgcgaactg cctattaagt ttttcacgag cctctatcat tgcaagcgct gccgctactc
     6061 catttgaagc gtgcttttta tcttcagatg gagctccaaa aaccgccatt agcgcgtcac
     6121 caatgtattt attcactatc cctccgtaac gtgttatagg ctcggacacg gcatcaaagt
     6181 atcggttaag ccagagcaca acagtttccg gatccatttt ttcggtcaag ccggtataat
     6241 tacgtatatc ggtaaagaga acagtcactg tcctctcttc tcctcccaga ttcatattac
     6301 cattcatcag gtggtctcgc acagaagggt caacaatctt gccaaaagta tctttcataa
     6361 gctctttttc gagaagctct cttccaagat cattgaggtt ttctgacaga agccccagct
     6421 cgtcgttaga ggtaacgggt aaatctatct tgtaatttgc tttgcggata tcgagtacgg
     6481 cttttttcat caaaagcagg ggttctcgaa aatatcccga tacaagatag gttaaagcag
     6541 cacctgatac cagaattacc acaatcatgg cgatgtaacc tgtataacta tcgctttctt
     6601 caatacgtga taaaacaaaa agcgaaaaaa tgtaaatcgg aaacaatgcg gcagtaatat
     6661 aatagacaaa gaatcttccg cgtatcgaaa agttggtatt gccagccagc ttcgaaagtt
     6721 tgccctcggg gaaaactctg ggaataacat atcttctgaa aaccatttct aaaataaaga
     6781 aagatacaac aaaactaaat attgcagcga gcagcatctc aagcgacgtt acgatcaggg
     6841 tagaagtcgc gatcagaggc tgcttaaacc agatgctaag aaacagtatc tcctccacta
     6901 tccacccact tataccaatt aaactgatct ttaaaggaac atcaagaata cgacccggtc
     6961 gaaatctttt ggccggttta cccattaatt tacgatacat tgcgatcaga attggagtca
     7021 gatatattat cacaagaaca gaggggataa ccatattgag ccactccatg tctcgtatca
     7081 tcgcgaaaag ctgctcactc gcaacccagt cggctggcgt aaagacaaga ggcccaacca
     7141 gaaatattat aactacagcc ccgacattgc taaggggatt aacaagtgat acggctaaca
     7201 tggtcatcac aacaattcga agcgattttt tctttttaac atccattaga acctcttgta
     7261 aaaaaagtct tatacataat aatcagacaa tttttcggta aaatttactt catcatactt
     7321 aaagctattc gatctcgaca gatgccgcac ccatatcata gtcatttctg ccaaccactt
     7381 ctccttctga tcttgcctct acataaacga agtagaggta ttttttgcct tcatattcac
     7441 agagagtcgg ttcacctacg ccaaaaattt cccctacaca atcctgttaa ccacaggaac
     7501 ccggacaatg agtgtagccg atataaaggc agtaagaata gcaataattg agcagagcaa
     7561 aaactttata attggcgcaa aatccagagg cataagaagt attgccgcgc caacacagaa
     7621 aaagggatga gtgatataaa ccacatatgc gcctcgtcca gcctccttta aaattgtcgg
     7681 tgctgtattg attttagttc taaatagttg caacagttta aaacagatgc caaaacagac
     7741 aaaaggatcc caaaaacagt acataagtga ctgaatcgta aagccaccgt taaaggcatc
     7801 cgtattgcca gaaactataa taactatggg aagcactacc atgagaacaa gtgaagttat
     7861 aaaccatctt tttgtaaccg aattgtcaat tttatcaagc cagttgtttc gtgaagcgac
     7921 tatccccgac acaaagagta aaacatataa aggaaaataa cccagcttta acgagagcag
     7981 agccttatct gcaggtaaaa atattcttat tacaaaggct actgatgcgg cgcaaatagc
     8041 gccaactatc aactttccat tagacggaaa atcggtcgtt ttattagact tgccaccaat
     8101 catgcgtaca atcgcataaa ctatggagaa tatcaaaagg gtttgtacaa accagagcgg
     8161 acccggtcca gcataatttg gcatgttttt gaaccagaaa gtgacaaaag acccattgta
     8221 ttcaccataa tagactctga ggatgtaaac gagcaatgga tttaaaatcg tataaaacag
     8281 aacaagagga ataccaaggc ggatcaacct gtcgcgaaga tacgaggcca tacccttttt
     8341 atcataagag ccgggagtaa aaaaaccaga gataaaaaag aagagcccca taaaaaaaga
     8401 ttggttaact gcggtaaaca gggttaagat caacgatgta agctcattgt gattaggatc
     8461 tataatatac cagtcaccgg atcctccgta tgttatggaa acatgatgag caataacaag
     8521 cattgttaaa aaaaccttaa gattatcaag atagtaataa cgttgtttca taacttctca
     8581 tccttataca agtatctgta aagcatatct gtagtttcat cgattaactt ctctttagat
     8641 attgtaacat ccgagaagac gataatatgc actatttctt caagggttgt ctgaatcaac
     8701 accgaaatgg tttcgatatc actgatacgc aggttatcga ttcttgatga taagaatttt
     8761 tttgcgagac tgatcgcctc aacgcgagcg ttattataaa gtgtcgctat atcggggtca
     8821 gatgcggaca taactgtcaa ctcttgatga agttggggat agacctcatg tgcttcgatc
     8881 agcttggtta tcagatagtt caggaatgtc ttgccatcat tgatagatcc gtttcccgga
     8941 ttttctatat catttaatac atcgcatatt ttttgattat acagctcaac aacttccata
     9001 aacagagctt ttttatcggc aaagtacata taaaaactgc cgatcgaaac gccggctcgc
     9061 tttgcaatct ctttagagtt ggttttataa taaccttttt cagaaaaaag ctgtaagccc
     9121 gcttcaatga tactgttttt tgactctttg cttctatctt gttgaggaac tcggatttta
     9181 ctattactca tggttaatta caatccagtt tatatttgta tgaccctgta aaataaatat
     9241 ttgaataaat gggaatcagt caagtaaaac atgattaaaa gttcatatta atcttgactt
     9301 tttatgaacc atagttcaca ttgtctcaac ataaatgaac tttggttcat gttttaccaa
     9361 ttatccaatc cgaaaggaga ggagtttaac aggaggtatt attatgtctg ttttagaaac
     9421 aatcagcgga agcggcatgg atcgtattcc gaatatcgct tttagaggaa tgtcttttct
     9481 tttcgcaatc tgggaaattt tcgcttcgcc tgaaaagttg ctaaaaaagt ttaaaatcaa
     9541 agagggaaac accgttgtag attatggctg cggcccgggc agttatatca aggcggcgtc
     9601 aacacttgcc ggacaaaacg gcatcgttta cgcagccgat gtacatgaat tggcgatagc
     9661 ggcagttaac aagaagattg agcgatatgg cttaacaaat gttaaaacgg cacaggtaac
     9721 cgattacaaa agcgaaatac ctgacaactg tgccgatgtt gtgtacgcgc tggatatgtt
     9781 tcatatggta aaaacgccga atctttttct aaaagagatt caccgaataa taaaaaaaga
     9841 tggtagactc tatattgagg acgggcacca atcacgtaaa aactctaaaa agaaaattga
     9901 aagctcaaag tcttggaaaa ttgaggaaga gtatcgaggt tttctggtat gttcgcctgt
     9961 ttaatcgtat tggttatctt gataattata tacgagcaga aatatctaat aggtactttg
    10021 ccttctgcta atggcaatta taaaaactat tttctcatct tctgatattt cataaaatag
    10081 cctaaaacta ccaaaccgat atctccaggt ttcgggttgg tagtccttta attttttgat
    10141 attattgcca aaatagggtc gatcagagag ttgaggataa atattggaat gtagtctatg
    10201 ctctacttta gttttttcat ttctattaag tttgttcttt tttttaatga aatggtcagt
    10261 ctcaaaaata cggtaattca ttaaacaaat ctaccgtttc tgttttttgc atcggtatta
    10321 ccacgcctta aagagtccaa taactcttta tcatttaaaa tatgatccat ttcatagtca
    10381 tcaacaaagt caatattttc gatatatttc agggttgctg tctcaatata attggataat
    10441 ggacgattat ccgcattagc atgttgttta ataattttat aaatttcatc gtctaatcgt
    10501 aatgttacag tcctggacat aataattcct ctagaataat aattgttcat tattattcta
    10561 atgtattcat gctttgtcaa tttgtcaaca gcaaacttag ataaattagc taccgagggg
    10621 tatctatgaa caattatcat tgaggtctga ttaatcaata acagctagac ggccattatc
    10681 tacagtgata ccatcttcca gcggaa
//
LOCUS       DAOTGZ010000079        10596 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_3064
            NODE_7436_length_10596_cov_5.104544, whole genome shotgun sequence.
ACCESSION   DAOTGZ010000079 DAOTGZ010000000
VERSION     DAOTGZ010000079.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868088
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 10596)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10596)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:22:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,785
            CDSs (total)                      :: 2,751
            Genes (coding)                    :: 2,732
            CDSs (with protein)               :: 2,732
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 12 of 19
            Pseudo Genes (internal stop)      :: 5 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10596
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7436_length_10596_cov_5.104544"
                     /isolate="AMR_MDS_3064"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..449)
                     /locus_tag="P5123_06785"
     CDS             complement(<1..449)
                     /locus_tag="P5123_06785"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRX16001.1"
                     /translation="MKKFYLLLIICFFACTASISAQETSDNSVDSDFGDFDFSSKTVT
                     DAPGSEEKNSVSIGGRLETESALSQKDRDLTRGYICLDLDLGYSNDNTEVKATLRGEK
                     DTLRTSTYYYTSPSAYEEARKSSYDVETIKKQSFSSGALDSVNTEDMR"
     gene            complement(609..1397)
                     /locus_tag="P5123_06790"
     CDS             complement(609..1397)
                     /locus_tag="P5123_06790"
                     /inference="COORDINATES: protein motif:HMM:NF028441.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_process: GO:0042953 - lipoprotein transport [Evidence
                     IEA];
                     GO_process: GO:0044874 - lipoprotein localization to outer
                     membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane lipoprotein-sorting protein"
                     /protein_id="HRX16002.1"
                     /translation="MKKITTFTLCALFLTGIQLSGAELTGRQIAEKVDAANKSETGII
                     TKGTIILKDDKTGKTEKRNSIVLAKTVSNNGKSIFRFTSGSQKGTTFLSEERNGKENL
                     QYVYLKSVGKARQVESSDKEKNFVDTDLSYESMGGLKIDDYNYKRLPDATFKGRDCYV
                     IENLPKRSNSKYSKHIVYTDKEYLIPVATKSYNKAGRLVKQLMANPATIKTLRKGLYV
                     PTEIIVKDLEEKHSTIVKAILVKETAFPNSVFNKNQLDSKLPME"
     gene            complement(1394..2194)
                     /locus_tag="P5123_06795"
     CDS             complement(1394..2194)
                     /locus_tag="P5123_06795"
                     /inference="COORDINATES: protein motif:HMM:NF012655.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HRX16003.1"
                     /translation="MVNCQENEKLVKHRILEAAKQEFAEMGFQGARTSRIASEAQVNK
                     AMIHYYFKDKEGLYKEVLRIVFGVNENAMAPILSKNWDISLPVKLHAILYFLFKVFTE
                     AVDNYFISFFFWELVETKQYLSRYVPEYVVPKLKAVKSIIDEGLEEGIFETENSKLLV
                     ANLFYFPMIYVAKNRLYSGRNIDPEKTFFGEDTAESVFSFHLSLIFKALCPSGRKLAI
                     PELPSDLKEMLDSLIEFMVISKQEGNAFKIINQLEKILTSKNIKEGKK"
     gene            2395..2763
                     /locus_tag="P5123_06800"
     CDS             2395..2763
                     /locus_tag="P5123_06800"
                     /inference="COORDINATES: protein motif:HMM:NF013767.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009927 - histidine phosphotransfer kinase
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Hpt domain-containing protein"
                     /protein_id="HRX16004.1"
                     /translation="MEESTDEVKKYELNSVQQKIFNRTGQNEKLYNMLIEIGLRTIPQ
                     YIQKLEKAIKENNPNDIKIISHSLKGALLNLAINDLATIAGEIQDLATVQSGMTQIAA
                     LNRRLLKEWQIVKLELQQKN"
     gene            complement(2760..3896)
                     /locus_tag="P5123_06805"
     CDS             complement(2760..3896)
                     /locus_tag="P5123_06805"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRX16005.1"
                     /translation="MKNRLTLMILALIIMGISLYTYQRLRFPSMENTQKAIHGKNAEL
                     YFSEYSKYFKKDALQSDQYPVVIDHMLKNVPGLSLVCAIDSFGNIVHGERNKLIIPQK
                     RAYNDIVNDLIKNKIANSQTFMSMHNKYYLYSVNCAPFTLHMIHSFKLNRKLLIQFIT
                     EAILVSSLVTGFLIFLISQIAKVFSTAVRINLPTTSEMKVCNNDNMLPETDINEHTDE
                     SIDVSETIESVNRSRVVPFETKESRKKQIRNFESYVFNTFNRIAITYRLNSLSLYLLD
                     NSSVSLRKIFELLGRAFVKTTASNSIENESCREIINALGDNSTIIRNGGRKIIIPVFH
                     EGELLGAVCVSNRELITGTVVTKVKEEIELLGSYLMSEILKNAG"
     gene            4358..5206
                     /locus_tag="P5123_06810"
     CDS             4358..5206
                     /locus_tag="P5123_06810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002763946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellin"
                     /protein_id="HRX16006.1"
                     /translation="MIINHNMSAINANRSLKFTHWDVNKSMEKLSSGIRINKAGDDAS
                     GLAVSEKMRTQIQGLRQAERNTEDGMSFVQTAEGYLDQTAQIIQRMRVLAIQSSNGIY
                     QASDRQLIQVEVSALIDEVDRIASQAEFNRFKVLTGEFSRVNPQASMWFHMGPNQNQR
                     ERLFIGTMTAQSFQMRDGAGNISLSLSSPAGANNAIGVLDNSLQKLSKQRADLGAYYN
                     RLELAAKGLMNAYENVQSAESRIRDADMAEVMVDFTKNQVLVQTGTSMLAQANIQSQS
                     VLRLLG"
     gene            5537..6379
                     /locus_tag="P5123_06815"
     CDS             5537..6379
                     /locus_tag="P5123_06815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002764028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellin"
                     /protein_id="HRX16007.1"
                     /translation="MRINHNMSAIFANRQLQLQTQAMDKTIEKLASGQAINRSGDDAS
                     GLAVSEKMRTQINGLFQAEKNTQNGLSFIQVAEGALQQINEILQRIRTLSVQSANGIY
                     SDNDRQQIQVEVSSLIDEIDRLASTAEFNRMKMLTGVFARGSAVGSMFFHVGPNKNQR
                     IRAYIRTMSADALNLIEGGVKKTISTAGAANNMIGTVDIALDELNRQRAELGAYYNRM
                     ENTAKSLSLSYENMLAADSRIRDADMAAEMVEFTKSRILVQSGVSMLAQANQKPQLIL
                     NLLK"
     gene            6508..6852
                     /locus_tag="P5123_06820"
     CDS             6508..6852
                     /locus_tag="P5123_06820"
                     /inference="COORDINATES: protein motif:HMM:NF015602.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar protein FlaG"
                     /protein_id="HRX16008.1"
                     /translation="MDINMVLSNTINRLSEPKVAPLHPVAEINLHDNSEPSGESRIEN
                     MSNVIDTLNKAAGSIDSRVSFQYNDKTKRIVMRIMDPETNELVRQVPTKEMIRLLERI
                     NEVTGVFLDEKR"
     gene            6973..7266
                     /locus_tag="P5123_06825"
     CDS             6973..7266
                     /locus_tag="P5123_06825"
                     /inference="COORDINATES: protein motif:HMM:TIGR00377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043856 - anti-sigma factor antagonist
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="STAS domain-containing protein"
                     /protein_id="HRX16009.1"
                     /translation="MELNIAEVYDDIIVSFSGEIDMLSIKGLKDSLFNLADYHKNVKI
                     NFENLDYLDSTGIGILLTLNRMVLENGNNLSLINVPDKIASILRLSSLNSILG"
     gene            7528..9612
                     /gene="fusA"
                     /locus_tag="P5123_06830"
     CDS             7528..9612
                     /gene="fusA"
                     /locus_tag="P5123_06830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011138410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor G"
                     /protein_id="HRX16010.1"
                     /translation="MPLTKLRNIGIAAHIDAGKTTTTERILYYCGVNRKLGETHDGQA
                     TMDFMKQEQERGITIASAAITATWKKNQINIIDTPGHVDFTIEVERSMRVIDGLIGLF
                     CSVGGVEPQSETVWNQAERYKVPRIACVNKMDRTGADFDEVCVQLEKYLDANPVPFHM
                     PIGAEEDFAGIIDLVEQKAVYYPSELEREEREIPAELKEAAEEARKHLIEKLADYDDE
                     IMELFFADEEVPADLIKRVARKCTMGRLITPVFCMSAYKNKGVQDMLDAVVAYLPSPI
                     DVGDVVGVDVDNPEKVHSRHPKDDDPFCALAFKLINDPYVGQQTFIRIYSGKLESGMQ
                     VLNSTKSKKERIGRIMRIHAKDREELTEAKAGEIVALIGMKETKTGDTLCDIKEPVIL
                     EKIHIPPSVIEMKVNPKNKNEEEKLGIALNKLTSEDPSFNVHVDEESNETIIAGMGEL
                     HLEIMVDRIKEEFGIEVEVGEPSVAYREAIQSSAEQDYKFSKQTGGKGMFAQCTIRFE
                     PNEQGKGFEFVNLIKGGAIPNEFIPSVKKGLEKTMAEGFLAGYEILDVKAVLIDGNFH
                     PVDSSDMAFQLCASMCFKAAYPKLNPVILEPIMKVEVNTPDDYLGDAIGDLNKRRGKI
                     GEMRRFRKGSQKVDARVPLMEMFGYSTSLRSLSSGRANYSMEFNSYEPVPANIQEKVI
                     KAAEERRAARNK"
     gene            complement(9706..>10596)
                     /locus_tag="P5123_06835"
     CDS             complement(9706..>10596)
                     /locus_tag="P5123_06835"
                     /EC_number="2.6.1.83"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015772927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LL-diaminopimelate aminotransferase"
                     /protein_id="HRX16011.1"
                     /translation="DTGNFQELFSNDISIAIPDPVYPVYVDTNVMAGRTGINNNGRYE
                     NLIYLDSNENNGFVPEPPSTKADLIYLCFPNNPTGATATKEQLKRWVDYALENRSLIL
                     FDAAYVSFIRDNNLPKSIFEIEGAKKCAVEFRSFSKNAGFTGTRCAFTVVPKDVMVYD
                     LKGNSHSLHALWNRRHSTKFNGVSYPVQKAAEAVYSEKGKQEVKALTDYYLENAAIIR
                     DKMISLGYSCTGGDNSPYIWIKSDMGSWEFFDYLLQNTGVVCTPGAGFGQCGEGYIRI
                     SAFNSRDNVIKAMEKIASLK"
BASE COUNT         3137 a         1930 c         2249 g         3280 t
ORIGIN      
        1 cgcatgtctt cagtattcac gctgtcaaga gctccacttg agaaagactg ttttttaatc
       61 gtttcaacat cgtagctgct tttgcgcgct tcttcataag ccgatgggct agtgtaataa
      121 tatgtggaag tgcgcagggt atctttttct ccgcggagag ttgccttaac ttcagtattg
      181 tcgtttgaat atcccaagtc aaggtccagg catatatagc ctcgtgttaa atcgcgatct
      241 ttttgagaca aggcactctc cgtttcaagg cgtccaccta tacttacgga gtttttttct
      301 tctgaaccgg gtgcgtctgt aactgtttta gaagagaagt caaaatcgcc aaaatctgag
      361 tcaacgctgt tgtctgaagt ttcctgtgca gaaatggatg ctgtgcaggc aaaaaaacag
      421 attatcagta ataaataaaa ttttttcatt gtagattttc cttaaaggca tccaagtgga
      481 taccgcgtat gtactccctg tttttttatg ctttcaggtc aaaaaaacag ggagggttgc
      541 caccctgtct gtagttgcag ctacggacag ggtctttcaa taaacattga atttattaac
      601 ttcagatcct attccatcgg aagttttgaa tcgagctgat ttttgttaaa aaccgaattc
      661 ggaaaagctg tctcttttac cagaatggct tttactatgg ttgagtgttt ttcctcaagg
      721 tctttaacta ttatctccgt tggaacgtag agtcctttgc gaagagtttt tattgtagca
      781 ggatttgcca taagctgttt tacaagacgt cccgctttgt tatagctctt cgttgcaaca
      841 ggaataaggt actctttgtc agtgtagaca atgtgtttgc tgtatttgga attgcttcgc
      901 ttgggcaagt tctctattac atagcagtct cggcccttga atgtagcatc aggaaggcgt
      961 ttatagttat aatcgtctat ttttagtcca cccatgcttt cgtaggaaag atcagtatct
     1021 acaaaatttt tctccttgtc agaactttct acctgacgag ctttgcctac tgatttcagg
     1081 tatacgtact gcaggttttc cttgccgttt ctttcttcgc tcaagaaggt ggtacctttc
     1141 tgtgaaccgg aagtaaaacg gaaaatagac tttccattgt ttgaaacagt tttagctaga
     1201 actatgctgt ttcgtttttc ggtttttccg gttttgtcgt ctttaagaat gatagtgcct
     1261 tttgtaatga tacctgtttc agacttgttt gccgcatcta ctttttccgc gatttggcgc
     1321 ccggttagtt ctgctccgga aagttgtatg cctgttaaaa aaagtgcaca cagtgtgaac
     1381 gttgtaattt ttttcatttt tttccctctt tgatattttt gctggtaaga attttttcca
     1441 gctgatttat tattttaaag gcgttaccct cctgtttgct gatgaccatg aattctatca
     1501 gtgagtcgag catctctttt aaatcacttg gcaattctgg tatggccagc tttctgccgg
     1561 atggacagag agccttaaaa atgagcgaca gatgaaatga gaaaacagat tctgcggtat
     1621 cttcaccgaa gaatgttttt tcgggatcaa tatttcttcc ggaatagagg cggtttttag
     1681 caacataaat catcgggaag tagaagaggt ttgctactag aagttttgag ttttcagttt
     1741 caaaaattcc ttcttccaaa ccttcatcaa taatactttt tactgccttc agtttcggaa
     1801 caacatactc tggtacgtaa cggcttaggt attgtttcgt ttcaaccagc tcccagaaaa
     1861 aaaagctgat aaagtaattg tctacggcct cagtaaaaac cttgaataga aaatagagta
     1921 tagcatgaag ctttaccggt agagagatat cccagttttt acttagaatt ggtgccatag
     1981 cgttttcgtt aacaccaaaa acaattcgca gtacttcttt gtacaggccc tctttgtctt
     2041 tgaagtagta gtgaatcata gccttgttaa cttgtgcttc agatgcaatc cgtgatgttc
     2101 gggcgccctg aaagcccatt tcggcaaatt cttgttttgc cgcttcaagt atacgatgct
     2161 ttactaattt ttcattttct tgacaattaa ccatatggtt aataaactaa ccatatggtt
     2221 aattgtcaag gccatttcga ttttttttga taatattttg ctaatactat caagaaatat
     2281 aaaaactgaa cggcgaagat gattttatat gataacagtc ccgttatatt aatgaaaaaa
     2341 cagaaagcaa atagtaccta aaagtatctg ataaaaaaaa aaccaaaaaa taaagtggaa
     2401 gagtcaacag atgaagttaa aaaatatgaa ctaaattcgg ttcagcaaaa aatctttaac
     2461 cgaaccggtc aaaatgagaa actttataat atgctgattg agattggttt aagaaccata
     2521 cctcaatata ttcagaaact tgaaaaggca ataaaagaaa ataaccccaa tgatataaaa
     2581 ataatctctc actcgttaaa gggggctctg ttgaaccttg caattaatga cctcgccacg
     2641 attgcaggcg agatacaaga cttggcaacg gtacagtcgg gcatgacgca gatagcagcg
     2701 cttaacagaa gactcttaaa agagtggcaa attgtaaaac tagagcttca gcaaaagaat
     2761 taaccggcat tttttagtat ttcactcatc agataagacc ccaatagctc aatctcttct
     2821 tttactttcg tcacaacagt tccggtgatc aattcacgat tactaacaca gacagctcca
     2881 agaagttcgc cttcatgaaa aacgggaata attattttcc gacctccatt gcggataatt
     2941 gtcgagttat ctcctaaagc gttaattatc tctcggcaac tttcgttttc tatactgttg
     3001 cttgcagttg tcttaacaaa tgctctgcct aagagttcaa aaatttttct taatgaaaca
     3061 gaagagttat ctagaagata gagtgatagt gaatttaatc tataggtaat ggcgattctg
     3121 ttaaacgtat taaatacata gctctcaaag tttctgatct gtttttttct gctctctttt
     3181 gtttcgaaag gtactactct tgatcgatta acagattcga ttgtttcaga gacatctatg
     3241 gattcatctg tatgttcatt tatatctgtt tctggtagca tgttgtcatt attgcatacc
     3301 ttcatctctg atgtagttgg taaattaatt cttactgcag tcgaaaacac tttggcaatt
     3361 tgagatatca gaaatattaa aaatcctgta acaagtgacg aaacaagtat ggcctccgtt
     3421 ataaactgaa taagcagttt acgattaagt ttaaaactgt gtatcatatg aagcgtaaag
     3481 ggggcgcagt ttacagaata aagataatat ttgttgtgca tagacatgaa tgtttgactg
     3541 ttcgctatct tgtttttaat taaatcgtta acaatatcat tgtaggctct tttttgtgga
     3601 attattagtt tgtttctctc tccgtgaaca atattgccaa aagaatcaat cgcgcacaca
     3661 agcgagagtc cgggaacatt ctttaacata tgatcaatca cgacagggta ctggtcagac
     3721 tgtaaagcgt ctttcttgaa atatttggaa tattctgaaa agtataattc cgcgtttttt
     3781 ccgtgaatgg ctttttgagt gttttccatg gaaggaaatc gtaaacgctg atatgtataa
     3841 agcgaaatgc ccataataat taatgctaat atcataagtg tgagtctatt tttcataatt
     3901 ttcgcctcat atcttttaca tatttatccc tataaccact ttcgacatta tctcttatgt
     3961 aattgagtgg tgtaaaaaaa attaataata attcgttttt ttattttttt cgcggttttt
     4021 tgttaaacag ataaatgtgt ataatatggt gccgtattat taaataaaga ctaatatatg
     4081 tcaatatatc tgtataacag ggctaagtaa acaacccgta ggatgcgata tttcgaacag
     4141 gggattttat atcctcgtgt cagatctttg aaaacgagta tcgattgtgt tgtgataatg
     4201 attattcggc agaatggttc cagccttata ttggcccaga tataagcctg gaacaggata
     4261 cggcaattat gtttattgcg gtatcaagca gctgtctgtc cctacccggc gggcagtggc
     4321 aaggacgcca agaaatagga tacaaaggag tgtaactatg ataatcaatc acaacatgag
     4381 tgctatcaac gcgaacaggt ctcttaagtt tacacattgg gatgtaaaca agagtatgga
     4441 gaagctatca tcgggtatca gaatcaataa ggcaggagac gacgcctccg gtcttgcagt
     4501 ttctgaaaag atgagaacgc agatccaggg attacgtcag gccgaacgaa ataccgaaga
     4561 tggtatgtcg tttgttcaga ctgctgaagg atatcttgat cagactgcac agatcataca
     4621 gcgtatgcgt gttctggcta ttcagtcttc aaacggtatc tatcaggcaa gtgatcgtca
     4681 gcttattcag gtcgaagttt cggctcttat tgacgaggta gatcgtattg cctctcaagc
     4741 cgaatttaac aggtttaagg ttcttactgg cgaattttca cgagttaatc ctcaggcttc
     4801 catgtggttt catatggggc caaatcagaa ccaacgtgaa cgtcttttta ttggtacaat
     4861 gactgcccag tcttttcaga tgagggatgg agcagggaat atttcccttt cactatcttc
     4921 tccggcaggt gccaataacg caatcggggt tcttgataat tctcttcaga agctgtcaaa
     4981 acagcgtgct gacctgggag cgtactacaa ccgtcttgag ctggctgcaa aaggtcttat
     5041 gaacgcttat gagaacgtac agtcagcaga gtcacgaatt cgtgacgcgg acatggcgga
     5101 agttatggtc gactttacca agaatcaggt tcttgtacaa actggaactt cgatgcttgc
     5161 gcaggctaat attcagtcac aatcggtact cagactgttg gggtagaaca gcaactggta
     5221 tctgacatat cgttctttga aattcaacgt gattgatcct tgagacgaat aatggatcaa
     5281 tctctcttgt gttatacaaa ctgtccaatg aagctggatc gtttccggcg ctctttgatg
     5341 agcgggaggc atcagtgagc gccggagtca tccggtctca ttgttgttaa aattttgggc
     5401 agttttggca acaaaaagga gaaaaccttc gtccggtaac ggattgccgt aaacatgatg
     5461 tgtgcggtaa aaaatcaggg attcttacaa aaattaaaaa aaggaatcct tttaaaacac
     5521 aagggaggga tttattatga gaatcaatca caacatgagc gctatattcg ctaacaggca
     5581 gcttcagttg cagactcagg ctatggacaa aacaattgag aaacttgctt cgggtcaggc
     5641 aataaatcgc tctggagatg acgcctcggg ccttgccgta tccgaaaaga tgcggactca
     5701 gatcaacggc ttattccagg ctgaaaagaa tacccaaaac gggctttctt ttatacaggt
     5761 cgctgagggg gctctccagc agataaacga aatattgcaa cgaatccgga cactgtcggt
     5821 gcagtccgct aacggtatct attctgataa tgacagacag cagatccagg tcgaagtttc
     5881 atcattaatt gatgaaattg accgactcgc ttctactgcc gaatttaaca gaatgaaaat
     5941 gctaaccgga gtttttgctc gaggtagtgc cgttggaagt atgttcttcc acgttggtcc
     6001 gaacaaaaat cagagaatca gggcttatat caggacgatg agtgctgatg ccttgaatct
     6061 gattgagggc ggggtaaaga agactatttc tacggctggt gcagcgaaca atatgatcgg
     6121 tactgttgat attgctcttg atgagctaaa tagacagcgt gccgagctgg gagcgtatta
     6181 taatagaatg gaaaatacgg caaagtcgtt aagtctttcc tatgaaaata tgcttgcggc
     6241 ggattcacgg atacgtgatg cggatatggc ggctgaaatg gttgaattta caaagagcag
     6301 gattcttgtt cagagtggtg tttcgatgtt ggctcaggca aatcaaaaac ctcagttgat
     6361 tttgaatttg cttaaataag aatccgcttt taagagcacc catgcagtgc ggtgtttctt
     6421 tacctcttcc ggtttgggag agccggaaga ggagattttt aaaatatttt cgggcaaaag
     6481 agtattaatt tagacaagga ggtcaatatg gatataaaca tggttttatc caacacgatt
     6541 aacagattgt cagaaccgaa ggttgctccg ttgcatcctg ttgccgagat taatcttcac
     6601 gacaatagtg agcctagcgg agaatccaga atcgagaata tgtcaaacgt tatagataca
     6661 cttaacaagg ctgccggaag catagatagt agagtctctt ttcaatacaa cgacaagaca
     6721 aaacgtattg ttatgcgaat catggatcct gagacaaatg aacttgttcg tcaggtacca
     6781 actaaagaga tgatacgcct gcttgagcga attaatgaag ttaccggcgt gtttcttgac
     6841 gagaagcgat aaatctgtta caaatttcat tgattgtaca gctttatcta taataacttg
     6901 actttttatg gatagacagg gtaggttgac tctgttgcaa atataatatt gttttattaa
     6961 atgggaataa ttatggaatt gaacattgct gaggtatacg atgatattat tgtctctttt
     7021 tcgggagaga ttgatatgct ttcaataaag ggtttaaaag acagcctgtt taacttagct
     7081 gattatcata aaaatgttaa aataaatttt gaaaatcttg attatcttga ttcaaccggt
     7141 attggtattc ttctgactct aaacagaatg gttcttgaaa atggaaataa tctctctttg
     7201 ataaacgtac cagacaagat agccagtatc ttgcggttaa gctctctaaa ctccattttg
     7261 ggataatttt atttcacaaa accgtaatta gttttggtga ctcttcagaa agcatctttc
     7321 ttccttagta ttactgatta atatgaaaaa aaattgacaa taatcacacg tctactaatt
     7381 atgccagtat ttatgacgga tctttaaggt gtaaacctac ctagacattc gttttgggat
     7441 tttaaaatga caatttgcaa tattggtcgt atccatcttg cagatagtct ttttttcccg
     7501 tgatattata aaattttgga gtatatcatg ccattaacaa aattacggaa tattggtatt
     7561 gccgcacata tcgatgccgg aaaaacaact accaccgagc gaattctata ttattgcggt
     7621 gtaaacagaa agcttggcga gactcatgat gggcaggcca ccatggactt tatgaagcag
     7681 gaacaagagc gtggtataac aatcgcttct gctgccatta cagctacatg gaaaaaaaat
     7741 cagataaata ttatcgatac accgggacac gttgacttca caattgaggt cgagcgttca
     7801 atgcgtgtta ttgacggatt gattggactc ttttgttctg ttggtggtgt cgagcctcag
     7861 agtgaaactg tctggaacca ggctgagcgt tataaagtac ctagaattgc atgtgttaac
     7921 aaaatggacc gaaccggtgc agattttgat gaggtatgtg ttcagcttga aaaatatctt
     7981 gatgcgaacc ctgttccttt tcatatgccg atcggcgcgg aagaagattt cgctggaatc
     8041 attgatttgg tagaacagaa agctgtttac tatccttccg aacttgaacg agaagagcgt
     8101 gagatccctg ccgagttaaa agaggctgca gaagaggctc gcaaacatct tatcgaaaaa
     8161 cttgcggatt atgatgatga aattatggaa cttttctttg cagatgaaga agttccggct
     8221 gacttaataa aaagagtagc tcgtaagtgt acaatgggtc gtcttattac accggttttt
     8281 tgtatgtcag catacaaaaa caagggtgtt caggatatgc ttgatgcggt tgtggcttat
     8341 cttccatcac caatcgatgt tggtgatgta gtaggagtcg atgtggataa tcctgaaaaa
     8401 gtacactcta gacaccctaa ggatgatgat cctttctgtg cgcttgcttt taaacttata
     8461 aacgacccgt atgttggtca gcaaaccttt atccgtattt actccggtaa attggaaagt
     8521 ggtatgcagg ttttaaactc gactaaaagt aagaaagagc gtattggtcg aataatgcgt
     8581 atacacgcta aagaccgaga agagttaacc gaagcaaaag caggggaaat tgtggctctt
     8641 attggaatga aagagacaaa aactggtgat actctttgtg atatcaaaga acctgttatt
     8701 ctagaaaaaa ttcatatacc gccttcggtt atcgagatga aggtaaatcc aaaaaataaa
     8761 aacgaagaag aaaaacttgg tattgcgtta aacaagctta cttccgagga tccatcgttt
     8821 aacgttcatg ttgacgaaga gtctaacgag acaattattg ccggaatggg agaacttcac
     8881 ctcgagatca tggtcgaccg tattaaggaa gagtttggaa tagaagttga ggttggagag
     8941 ccttcggtag catatcgtga ggcaattcag tcttctgcag agcaagatta caaattctct
     9001 aaacagaccg gtggtaaggg tatgtttgcc caatgtacaa tccgttttga gcctaacgag
     9061 caagggaaag gttttgaatt cgttaacctt attaagggtg gagcgattcc gaacgaattc
     9121 atcccttctg ttaagaaagg tcttgagaag actatggcgg aaggattctt ggccggatac
     9181 gagattcttg atgttaaagc ggttcttatc gatggaaatt tccaccctgt agattcatcg
     9241 gatatggctt tccagctttg tgcatcaatg tgttttaagg ctgcttatcc taagcttaat
     9301 ccagttattc ttgagcctat catgaaggtt gaggttaata cacctgatga ctatcttggg
     9361 gatgcaatag gcgaccttaa caaacgtcgt ggaaaaatcg gtgaaatgcg tcgttttcgt
     9421 aagggatctc aaaaggtaga tgcccgtgtt cctcttatgg agatgtttgg ctattcgact
     9481 tcacttcgtt cgttatcatc tgggcgcgcg aactattcaa tggaattcaa ctcttatgag
     9541 cctgttcctg cgaatataca ggagaaggtt attaaggctg ctgaagagcg aagagctgca
     9601 cgcaacaagt aaaacagaga aaatagaatt atatataaaa agagataccg aaaggtatct
     9661 cttttttatt atttttagcg ttaaagtatt ctaatgattt tgcaattatt taagtgaggc
     9721 aattttttcc atggctttta taacattatc acgcgagtta aaagcactga tacgtatata
     9781 cccttcacca cattggccaa aaccggcacc tggtgtgcac acgacccctg tattttgaag
     9841 aagataatca aaaaattccc atgagcccat gtctgactta atccatatat aaggactatt
     9901 atctccacca gtgcagctgt aaccaagtga aatcattttg tcgcgaatta ttgccgcatt
     9961 ttcgaggtag tagtcggtaa gagcctttac ctcttgtttg cctttttccg aataaacggc
    10021 ttccgcggct ttttgtacag ggtaagaaac tccgttaaac ttggtgctgt gtctgcggtt
    10081 ccaaagagca tgaaggctgt gagaattacc tttcaagtca taaaccataa catctttcgg
    10141 tacaaccgta aaagcacatc tggttcctgt gaaccccgcg ttttttgaaa aacttctaaa
    10201 ttcaactgcg catttttttg cgccttctat ttcaaagatc gactttggaa ggttgttatc
    10261 acgaataaag gagacatagg ccgcgtcaaa tagtataagg gatctgtttt ccaaagcata
    10321 gtctacccat ctcttaagtt gctcttttgt ggcagttgct cctgtcggat tattggggaa
    10381 acagaggtaa atcagatctg cttttgttga aggaggttcc ggtacaaatc cattgttttc
    10441 gtttgagtca agataaataa gattttcgta acgtccattg ttattaatac ctgtgcggcc
    10501 ggccattaca tttgtgtcaa cataaaccgg ataaactgga tcgggaatgg ctatgctaat
    10561 atcatttgag aaaagttctt gaaagttgcc ggtatc
//
LOCUS       DAOTGZ010000080        10332 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_3064
            NODE_7667_length_10332_cov_5.421232, whole genome shotgun sequence.
ACCESSION   DAOTGZ010000080 DAOTGZ010000000
VERSION     DAOTGZ010000080.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868088
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 10332)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10332)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:22:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,785
            CDSs (total)                      :: 2,751
            Genes (coding)                    :: 2,732
            CDSs (with protein)               :: 2,732
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 12 of 19
            Pseudo Genes (internal stop)      :: 5 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10332
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7667_length_10332_cov_5.421232"
                     /isolate="AMR_MDS_3064"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..408)
                     /locus_tag="P5123_06840"
     CDS             complement(<1..408)
                     /locus_tag="P5123_06840"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRX16012.1"
                     /translation="MNKTGVFEKYLAKAVMKDDELPSIRKIEGFFKGLSEEELISLID
                     AVISSNDHDEFQLFLYYLLLFRENRNIQKFINSPRLNIELLERVVLFTYGYCTLKDYP
                     TEVIFDEILYFIDQQRLLSLLIDGKFVHRDKLLL"
     gene            complement(627..2639)
                     /gene="metG"
                     /locus_tag="P5123_06845"
     CDS             complement(627..2639)
                     /gene="metG"
                     /locus_tag="P5123_06845"
                     /EC_number="6.1.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011164204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004825 - methionine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006431 - methionyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine--tRNA ligase"
                     /protein_id="HRX16013.1"
                     /translation="MNKKRKILVTSALPYANGDIHLGHLVEYLQTDFWVRFQKMRGHD
                     CHYMCADDTHGTPIMISARDRGITPEALIAECHDRHYNDFKNFKIEFDNYYTTNSPEN
                     RELSSEIFLAMKEKGHIEERTISQLYCAHDKMFLPDRFVTGVCPSCGAEGQYGDSCDV
                     CSATYSPSDLKDAACVICGKKPELRESVHLFFKLNNFRDFLKGWVKDHASRETANKLE
                     EWLSEDLRDWDISRDAPYFGFEIPGYKDKFFYVWVDAPIGYISATKNWCDKNGRDYTE
                     FWKAEDAELYHFIGKDIVYFHTLFWPAMLHNAGYKTPDSVFVHGFLTVNGEKMSKSKG
                     TFIKARTYLDHLSPAYLRYYYACKLNATIDDVDLSFDDFVTRVNSELIGKITNIASRS
                     IQMLKKLDLKTGVLSDDGKELVQKAQERSESIARHYEDRNFSKAIKEVRELAEDANRY
                     IDHHEPWKHVASDPEKVRSILTALINVFRIITVYLKPVLPAYAAEVEELLNSGVLTWN
                     SVNEVLENHVVKPYKHLLNRIEKESCDAIIESEVKAAANAKNANKTSSPSVADTITFD
                     DFAKVDLRVAEVINAEDVEGADKLLKLTIWLGGDDRRTIFAGIKKYYSPEQIKGKLIA
                     VVANLAPRKMKFGLSEGMLLAASAEDKSTVLLLSPDSGAKPGQKIS"
     gene            2867..3967
                     /locus_tag="P5123_06850"
     CDS             2867..3967
                     /locus_tag="P5123_06850"
                     /inference="COORDINATES: protein motif:HMM:NF024242.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HRX16014.1"
                     /translation="MNSSSLLISSILGLAGGIVTLIFLTLYKGENPGLTKTAALLTTT
                     QALQMIFSLVFMFTGWTDKFSGLYWTTSLTSTVFMLYLLKSCCRKNTFKDPKSLSHLI
                     TLLFAITASIFFVIPDFTFASFVSHEQVERVLLVFSTALIILYFFFAVKEYKNYCDSI
                     QNIYSSINYYATQRLFFLIVALFLVVCLSIFSAAIFTFASPLQIRNLYPIASLLRVIP
                     LYGFYVLVIALVAAQPLPIVEDNEDEYFTQNTISFKELYSKTNELIIRKKLYLEPDID
                     ITKVAAILKCTPCELVNAIKEKTGGSFYYFVNTIRAKKSIEMLNDPISKSFPFVTIAC
                     QAGFSSEIAFNRVFKKVTGMKPNDYRRKQEQG"
     gene            complement(3998..4456)
                     /locus_tag="P5123_06855"
     CDS             complement(3998..4456)
                     /locus_tag="P5123_06855"
                     /inference="COORDINATES: protein motif:HMM:NF018028.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RDD family protein"
                     /protein_id="HRX16015.1"
                     /translation="MRYAGFWRRAAAFFIDMLVVGAVAEITGQVFLVVFGGPNEQGIM
                     AWGYANMVMYGLFWVYFALMESSSFHATIGKLFFDVRVIADNDKEMNFKRAVIRNLCK
                     VPSAMILLIGFFMAGWTSKKQALHDIISGSLVIDKPGHNIIFQKKSDNGE"
     gene            complement(4520..5752)
                     /locus_tag="P5123_06860"
     CDS             complement(4520..5752)
                     /locus_tag="P5123_06860"
                     /inference="COORDINATES: protein motif:HMM:NF028438.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5107 domain-containing protein"
                     /protein_id="HRX16016.1"
                     /translation="MKKCVSDVNRNLLIITIIFLTAGCALSDLLKSVGPVEGQSEDMV
                     QKIHQPDSYFVPIEDFENGQMKLYTGSYKFSHYQYTQVKGKPELVSVGKEIITTELRT
                     HVIENRYIKVALLPQFGGRIISIIDKKSGRELLYQGKDLRIIRSNAGNLHNDWHMGLG
                     GVTPSLMQGDHGISWCMPWNSELVKADKELISIKMSYAVPDLPGALCSAVVSVDSKHS
                     RVKTDYTLSASGESAVSWYYRTSYRFAYFAGNSLFMPKLYTGDRNWFSYIDKNINAGV
                     VRVATGYPSPGIIGLAENFTGGLFSLPLICDLDPGQSVSWSEFLYPVNDMPIIMSAGN
                     FCAYALTKEKNHRFYFNYPGVEYEIQFKRNGRLMFARTVTPRGGEAFEVSARSLPAPA
                     DYLTIIFKGKEVLDSRDL"
     gene            6390..8450
                     /locus_tag="P5123_06865"
     CDS             6390..8450
                     /locus_tag="P5123_06865"
                     /inference="COORDINATES: protein motif:HMM:NF014904.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="[Fe-Fe] hydrogenase large subunit C-terminal
                     domain-containing protein"
                     /protein_id="HRX16017.1"
                     /translation="MKQMKPVVNVNIDNCVNCHACVAACPVKYCNDASKDYVEINHDM
                     CIGCGSCVKACTHNARLIVDDFDQFMSDRNKHKFFAIVAPAVAANFPGNYLNLNGFLE
                     SLGVQAFFDVSFGAELTVKSYIEAIKTKNPKTVIAQPCPAIVSYVEIHKPELLPYLAP
                     ADSPMLHTIKMVKEFYHQYKNHRVIVVSPCLAKRREFDSTGLGDTVYNVTYQSLNSYL
                     NDNRKKLSDYKEIDFSNPPAERAVLFSTPGGLLKTAERDIPGIAEKTRKIEGTHTIYH
                     YLDSLKESIDHGIAPLLVDCLNCEAGCNGGPGTLNIDKSVDEIEHYINERNKQMQKKY
                     QQSSTEKTSKKINKLLSKYWKPGLYDRSYKNRSDQYTIKTPNKSELKKIYESMKKFSD
                     AEIVNCNSCGYGSCEGMAIAIHNGLNTPENCHYYKQKLLIEEHFKTEEEHKKAVQAAE
                     EIEQSRIEIEKEHQTKMDIATQVSATTTEMENNNRAIAKMTENLSGLSRDQEKSLTLL
                     FSTLSEAGKITSEFKSIAEAINDVAEQTDMLALNASIEAARAGDVGKGFAVVSDEVKK
                     LAENTQDEIRKIGSFTDNLQKVFNRISDSSNDVFKNFEKMAQLTTEVTAATEEMAAAT
                     TAVNNEIDKLVDNDNSQQQIDSLFNESQDPDDTIQDKPDREPTHPDYMVSEDNDEDED
                     KKLA"
     gene            complement(8465..9691)
                     /locus_tag="P5123_06870"
     CDS             complement(8465..9691)
                     /locus_tag="P5123_06870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014789817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HRX16018.1"
                     /translation="MMKIKADLLYDGFTETESRYITIEGDRIVSVSSEPCNFDYEGVV
                     TPAFIDPHSHIGMERQGEPGEEGETNDEIDQITPLNNPLNSIYFDDRAFKDAVDFGVL
                     YSCVVPGSGNLLGGKAMIIKNFALNRHEAFVKHYGYKMALGFNPRSTGNWKGTRPTTR
                     MGVYAHLESTFDEVLQKREKERCSLEDSLSDIEEKFNDDKIDEETRQERIEIVHLEHE
                     LEFSPTEKALLEILDGKKTCKVHVHKEDDIIYLLELVEKYELKITIDHACDVFNREIF
                     ELIAQKNIPVVYGPLGSFDYKVELKHAYYQNPALLMGSGVDYSLMTDHPVVLSHHLRD
                     SLKYFLIQGMAPAEAIGLITSASARILGLDKDLGSIAEGKLASLVVWDKDPFNLGAFP
                     RAVIAEGKVIRDFTSK"
     gene            9868..>10332
                     /locus_tag="P5123_06875"
     CDS             9868..>10332
                     /locus_tag="P5123_06875"
                     /inference="COORDINATES: protein motif:HMM:NF019559.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lysoplasmalogenase family protein"
                     /protein_id="HRX16019.1"
                     /translation="MTHNYQIFIALYAFFVLWALVLANRRSCIFVNIKPIPVLLCCIL
                     LTSIVLNIEVHIALYKYTVPLALFFGCVGDYLITRLDDFRYGMILFFAGHCIYIIFFM
                     GITGSNFDHRAFLALLPLAIYLPLLIRKLPTGYKNLSLLVTTYAVTLGIMVCT"
BASE COUNT         3243 a         2261 c         1828 g         3000 t
ORIGIN      
        1 aagtagcaac ttgtctcggt gaacaaactt tccatcaata agcagactga gtaacctttg
       61 ttgatcaata aaatatagaa tctcgtcaaa tataacctct gttgggtaat ctttaagcgt
      121 acagtaacca tatgtaaaaa gaacaaccct ctcaagaagc tcgatattaa gacgtgggga
      181 attaataaat ttctgaatat tccgattttc tctaaaaagt agcaggtagt ataaaaaaag
      241 ctgaaactca tcatgatcgt ttgatgatat aaccgcgtct attaggctga taagctcttc
      301 ttcggacaag cctttaaaaa acccctctat tttgcgaatg gaaggcagtt catcgtcttt
      361 catgactgcc tttgcaagat atttttcaaa aacacccgtt ttgttcatag tcagacacat
      421 taccttgtta ctcatatata gaaaaaagat gcaaagcgtt ttttaagcca gtattaggat
      481 tttccgaacc aaaacaactc gtaaaacgat acgaaatatt caaatataac cgaataaccg
      541 gttatttacg ttaaataata acacaattga cagttaaaac aagatttttt gaacattttc
      601 aattcaaaga tcctgtttaa cattaattaa gaaattttct gtcctggttt tgctccagaa
      661 tcaggcgaca agagaagaac tgtactcttg tcttcggctg atgctgccaa aagcatacct
      721 tctgatagtc caaacttcat ctttcttgga gcaagatttg ccactacagc gataagtttc
      781 cccttgatct gctctggaga ataatacttc tttattcctg caaatattgt tcttcggtca
      841 tcaccgccaa gccagattgt aagcttgagt agcttatccg cgccctctac atcctcggca
      901 ttaattacct ctgccacacg aagatcgacc ttggcaaaat catcaaatgt tatcgtatca
      961 gctactgacg gcgagcttgt tttattcgca ttttttgcgt tcgcagcagc cttaacctca
     1021 ctttcaataa tagcatcaca tgactctttt tcgattcggt taagcagatg tttgtacggc
     1081 ttaactacat gattttcaag tacttcattc accgaattcc aggtcaaaac tccgctgtta
     1141 agcagctctt ctacctcggc agcgtaggcc ggaagtacgg gcttaaggta aacagtgatt
     1201 attcgaaaca cattaataag ggcagtcaat attgagcgta ctttttcagg atcagaagca
     1261 acatgtttcc atggttcatg gtgatcaata taccggtttg catcttccgc aagttccctc
     1321 acctctttga ttgccttcga gaaattgcgg tcttcataat gacgggcaat actttccgaa
     1381 cgttcctgag ccttttgcac aagctctttg ccgtcgtcgg acaaaactcc agttttaagg
     1441 tccagttttt ttagcatttg tatgcttcgc gacgcaatat tggttatttt gccaataagc
     1501 tctgaattaa ccctggttac aaaatcatca aaactcagat cgacatcatc tattgttgcg
     1561 tttagcttac aggcatagta ataacgaaga taggcgggcg aaagatgatc aagatatgta
     1621 cgagccttga taaatgttcc ttttgatttg ctcatctttt cgccgtttac agtcaaaaac
     1681 ccatgtacaa ataccgaatc cggtgttttg tacccggcat tatgaagcat agccggccaa
     1741 aacagggtat gaaaatagac gatatccttg cctataaagt gataaagctc cgcatcttcc
     1801 gctttccaga actccgtata atcacgaccg tttttatcac accagttttt tgttgccgag
     1861 atataaccta ttggagcgtc tacccaaacg taaaaaaact tgtctttata acctggaatc
     1921 tcgaatccga aataaggcgc atctcgtgat atatcccagt ctctcaggtc ttcagaaagc
     1981 cactcttcca gcttgttggc tgtctctcgt gacgcgtgat ccttgaccca accttttaag
     2041 aaatccctga aattgttcag tttaaaaaat agatgcacac tttctcgaag ctccggtttt
     2101 tttccgcaaa taacacaagc cgcgtccttt aaatcggaag gcgaataggt agcactacaa
     2161 acatcacaag aatcaccata ctgaccttcg gcaccgcaag atgggcacac ccctgtcaca
     2221 aagcgatctg gcaaaaacat cttgtcgtgc gcgcaatata gctgagaaat tgttcgctct
     2281 tcgatatgcc ccttctcttt catcgccaga aagatctctg aagagagctc acggttttca
     2341 gggctgtttg ttgtataata gttatcaaac tcaattttaa agtttttaaa atcattatag
     2401 tggcgatcat ggcattcggc tattaaagcc tccggagtga tgcccctatc gcgcgcactt
     2461 atcatgatag gagtaccgtg tgtgtcatcg gcacacatgt agtggcagtc gtgcccgcgc
     2521 atcttttgaa aacgcaccca aaaatctgtc tgaagatatt cgacaagatg accaagatgt
     2581 atatcgccgt tggcatacgg caaagcagaa gtaaccagta tttttctttt tttgttcatt
     2641 tattatttcc ttacaattag tataattaca cacaaaaaca acatttcatc atgttagcag
     2701 aatcatgcga gtagttgtca ataagataat gtgttaatct ttcaattagc agtaaatcaa
     2761 aacccgttca taaaccctgt ttatataaat ttatggacaa aacggcaaag atcaggtcta
     2821 ttgtagctaa ttgaaaagat tatagcaaaa atatactgga tacaacatga attcatcatc
     2881 actccttata tcgtctatac tcggtttggc cggtggaatc gtcactctta tttttttgac
     2941 actctacaaa ggggaaaacc caggattaac aaaaaccgcg gctcttctca ctactactca
     3001 ggctttacaa atgattttta gccttgtttt tatgttcaca ggttggacgg acaaattttc
     3061 tgggctctat tggacaacat cgcttacaag tacggtattt atgctatatc tattaaagag
     3121 ttgctgccga aaaaacacat ttaaagaccc aaaatcactt tcacatttaa taacacttct
     3181 tttcgcgatt acagcgtcga tcttttttgt aataccagat tttacgtttg ccagttttgt
     3241 aagccacgaa caggtagaac gtgttcttct ggttttttct acagctttaa ttatactcta
     3301 ttttttcttt gctgtgaagg aatacaagaa ctattgcgac agtattcaaa atatatattc
     3361 atcaataaac tattacgcca ctcaaagact cttttttctg atagtggcgc tttttctagt
     3421 ggtctgtctt tctatttttt ctgctgcgat ttttaccttt gcgtcccctt tgcaaataag
     3481 aaatttatat ccgatagcca gcttgcttag agtaatacct ctatatggtt tttatgtctt
     3541 agtaatcgca cttgttgccg cacagcctct tccaattgtt gaagacaatg aagacgagta
     3601 ctttacccag aatacaattt ctttcaaaga actgtacagt aaaaccaacg aactaataat
     3661 cagaaaaaaa ctgtaccttg aaccggatat tgacattact aaagttgcag caatactaaa
     3721 atgtactcca tgtgagcttg tgaatgctat taaagaaaag acgggaggaa gtttttacta
     3781 ttttgtaaac actatacgcg caaaaaagtc catagagatg cttaatgacc cgataagcaa
     3841 aagcttccct ttcgtaacaa tcgcctgcca agctggattc agttcagaga ttgccttcaa
     3901 ccgagtgttc aaaaaagtta ccggaatgaa acctaatgat taccgcagaa aacaggaaca
     3961 aggttgaccg agcaaacacc taatttgtaa cccgaaccta ttctccgtta tcactttttt
     4021 tctgaaaaat aatattgtgc cccggcttgt caatcacaag tgaaccggaa ataatatcat
     4081 gtaatgcctg tttttttgat gtccagccgg ccataaaaaa gccaattaat aaaatcatag
     4141 cggaaggcac tttacataaa ttacgtataa ccgccctttt aaaattcatc tctttgtcgt
     4201 tgtccgcgat aaccctaaca tcgaaaaaaa gcttacctat agttgcatga aatgatgatg
     4261 attccattag cgcgaagtag acccaaaaaa gaccatacat aaccatattc gcataccccc
     4321 aagccataat gccctgttcg ttaggaccac cgaacacaac aagaaaaacc tgacctgtta
     4381 tctcggcaac agcgccaaca acaagcatat cgataaaaaa ggctgccgca cgcctccaga
     4441 aaccagcata tctcataata atacctcaat cgttattttg actttattca agtaagacct
     4501 ctaacataaa tttattcgat caaagatccc tactatccag cacttctttg cccttaaaaa
     4561 taatagtcaa gtaatcagcc ggagctggaa gcgatcttgc gcttacctcg aaagcctcgc
     4621 ccccccgagg cgtcactgta cgggcaaaca ttaaccttcc attgcgctta aactgaatct
     4681 cgtactccac acccggatag ttaaaataaa accggtgatt cttctctttt gttagcgcat
     4741 aagcgcaaaa attaccagca gacatgatta tcggcatatc gttaaccgga tagagaaact
     4801 cactccagct cacggattga cccggatcca agtcgcatat aagaggtaat gaaaaaagac
     4861 cacctgtaaa attttcggcc aggccaataa tgccaggtga tggataaccg gttgcgactc
     4921 gaaccacacc ggcatttatg ttcttgtcaa tatatgaaaa ccagttcctg tcacccgtgt
     4981 aaagtttagg cataaacagt gaattacctg caaaataggc aaaccggtac gaagttctgt
     5041 aataccaaga tacagctgac tctcccgatg cacttaaggt ataatctgtt ttaacacggg
     5101 agtgtttact gtcaacagaa actacagccg aacatagtgc acccggtaaa tcaggaacag
     5161 catagctcat ctttatgctt atcaattctt tatcggcctt taccagctca gagttccacg
     5221 gcatgcacca gcttattccg tgatcaccct gcatcaaaga aggggtgact ccgcctagac
     5281 ccatatgcca atcattgtga agatttccgg cattacttcg aataattcga aggtcttttc
     5341 cctgatagag aagctcacgc ccactcttct tgtcgataat agagattatt ctgccaccaa
     5401 actggggcag cagggcgacc ttgatataac gattttcaat aacatgtgta cgtaattcag
     5461 tagtaataat ttctttgcca acagagacaa gttctggctt gccctttact tgagtgtact
     5521 gataatgact aaatttataa ctaccggtat acagcttcat ctggccgttt tcgaaatctt
     5581 cgataggcac aaaatagctg tccggctgat gaatcttttg taccatatct tccgattgtc
     5641 cctcaaccgg tcctaccgat tttaatagat cggacaaagc gcaaccagct gttaaaaaga
     5701 taattgtaat aattagtaaa tttctattaa catcagatac acattttttc attacgatgc
     5761 cccttggtgc gattttacat ctcttaccgc tcgttcgtgt ttccaataac acattaaacg
     5821 gtggcagcaa ttatattttc ttaggcaaga caaatcttat aatttttcaa accaacgcat
     5881 catttactta tcacagggtt tactggcaag gattttttgg taatgatcca atcacgaact
     5941 atgtgatcgg atattggttt ccaaatttct tatggccgaa atgcattttt tcattgaata
     6001 tttttaatct acacaccgac gcaatagcta tcaagacaag atggtattac tagacacgcg
     6061 tgtagaatca cactatgaat acggcactaa tcacaattat atacttgaca ccacaaaatc
     6121 ttgcaataat tatgtaacaa tttgcccggt tataatatta aacagtattg ttacgcagag
     6181 ttatttctgc cgttacaatt attaattcta atgatagtta aatttatcag ttattgtttt
     6241 aataataaat accgatatta ttattatggt gtccatttta ttaataaaca ataattgtta
     6301 attagctatt tatttttttt taaacaattg tacttataaa tataaatatt ccaaagcata
     6361 ttttcaaaca aaataaagag agccttaaca tgaaacagat gaaacccgta gtaaacgtaa
     6421 atattgataa ctgtgtaaac tgtcacgcct gtgtagcggc gtgccccgtc aaatattgca
     6481 acgatgccag taaagattat gttgaaataa accacgacat gtgtataggt tgcggcagct
     6541 gcgttaaagc ctgtactcat aacgcgcgat taatcgtaga cgattttgat cagtttatga
     6601 gtgatcgaaa taagcacaag ttcttcgcaa ttgttgcacc tgctgttgcc gcaaactttc
     6661 ctggcaacta ccttaatctt aatggatttc tggaatcact tggagttcaa gccttttttg
     6721 atgtaagttt tggagccgag ctgaccgtta agtcatatat agaagcaatc aagacaaaga
     6781 accctaaaac agttattgcg caaccttgtc cggccattgt tagttatgta gagattcata
     6841 aaccggaact attgccatac ctggctccag ccgatagccc gatgctgcat acaataaaaa
     6901 tggtaaaaga gttctatcac caatacaaaa accatagggt aatcgtagtt tccccctgcc
     6961 ttgcgaaaag acgagagttt gactcgacag gccttggaga taccgtttat aacgtaacat
     7021 atcagtcatt aaacagctat cttaacgaca accgtaaaaa attgtctgac tataaagaga
     7081 ttgatttttc gaacccgccg gcagagcggg cggttctttt ttcgacaccg ggagggcttc
     7141 tgaaaactgc cgaacgtgac atccctggta ttgccgagaa aacacgaaaa atagaaggta
     7201 cgcatacaat ataccattat ctggactctc taaaagagtc tatcgaccac ggcatagctc
     7261 ctcttcttgt agactgtcta aactgtgaag ccggttgtaa cggtggaccg ggaaccctaa
     7321 atattgataa gtctgtagac gaaattgaac actacataaa cgaaagaaac aaacagatgc
     7381 aaaagaagta tcagcagtca agtaccgaaa aaacatcaaa aaaaataaac aaactacttt
     7441 ctaaatattg gaaaccgggt ctttacgacc gtagttacaa aaaccgttcg gatcaatata
     7501 ccattaagac tcccaacaag tctgagctga aaaagatata cgaatccatg aaaaaattct
     7561 cggacgccga aatagtcaac tgtaactcgt gcggttacgg aagctgcgaa ggcatggcta
     7621 tagccataca caacggactt aacacacccg aaaactgtca ctattacaaa cagaaactgc
     7681 taatcgaaga acatttcaaa acagaagaag aacataaaaa agccgtacaa gccgccgaag
     7741 agatcgaaca aagcagaatc gaaatagaaa aggaacacca aacaaaaatg gacattgcca
     7801 cccaagtatc ggcaacaaca acagagatgg aaaacaacaa cagggccatt gccaagatga
     7861 ctgaaaacct ctccggttta tcacgcgatc aggaaaaaag tcttacacta ttgtttagca
     7921 cgctatccga agccggcaaa attacatcag aattcaaatc cattgccgag gcaataaacg
     7981 atgttgccga gcaaaccgat atgcttgcac ttaatgccag tattgaggcc gcacgcgcgg
     8041 gagatgttgg caaaggattc gcggttgtat cggatgaagt aaaaaaactt gccgaaaaca
     8101 cccaggacga gatccgcaaa ataggctctt ttacagacaa cctgcaaaaa gttttcaacc
     8161 gaataagcga ttcatcaaat gatgtcttca aaaattttga aaagatggca cagcttacca
     8221 ccgaagtaac cgcagcaacc gaagagatgg cagccgcgac aacagcggtt aataacgaaa
     8281 tagacaagct tgtcgataac gacaattcac aacagcagat tgactcttta tttaacgaat
     8341 ctcaggatcc cgatgataca attcaggata agccggatcg agagccaact catcctgatt
     8401 acatggtttc agaagataac gacgaagacg aagacaagaa actggcatag caacgcccta
     8461 cttgctattt tgaagtaaaa tcccgtatca ctttcccctc agcgataacc gcgcggggga
     8521 aggctcctaa attaaaaggg tctttatccc agacaacaag actagcaagc ttgccttcgg
     8581 cgattgatcc caaatcttta tccagaccta aaattcgagc actagccgat gttatcaagc
     8641 ctattgcttc ggctggagcc attccttgaa taagaaaata ttttaatgaa tcacgcaaat
     8701 gatgagacag tacgaccgga tgatccgtca taagtgaata gtcgactcca cttcccatta
     8761 aaagagccgg gttttggtaa taggcatgct tcaactcgac cttgtaatcg aatgaaccta
     8821 gcggaccgta tacaacagga atattttttt gagctattaa ttcgaaaatt tctctgttaa
     8881 atacatcgca agcatggtcg atagttattt ttagttcata cttctcgacc agttcaagca
     8941 gataaataat atcgtcttct ttatgcacat gaaccttgca tgttttcttt ccgtcaagaa
     9001 tttcgagtaa tgctttctcg gtcgggctaa actccaactc atgttcaaga tgcactatct
     9061 caattctttc ctggcgtgtc tcttcgtcaa ttttatcatc gttaaacttc tcttcgatgt
     9121 cgcttaatga atcctcgagc gagcagcgct ccttttctct tttttgcagc acctcatcaa
     9181 aagttgattc aagatgagca tatacgccca ttcgggttgt agggcgtgtt cctttccagt
     9241 ttccggtaga acgcggatta aaccccagag ccatcttgta tccataatgt tttacaaaag
     9301 cctcatggcg attaagggca aagtttttta taatcatggc cttgcccccc aaaaggtttc
     9361 cacttcctgg aacaacacaa ctataaagaa caccaaaatc gaccgcgtcc ttaaacgccc
     9421 ggtcatcaaa atagatgctg ttaagcggat tatttagcgg agtaatctga tctatctcgt
     9481 catttgtttc gccctcttct ccgggctcac cctgacgttc cataccgata tgcgaatggg
     9541 gatctataaa tgccggagta acaaccccct cgtaatcaaa attgcagggc tcgctactta
     9601 ccgaaacaat tcggtcaccc tcaattgtga tataacgact ttctgtttct gtaaaaccgt
     9661 cgtaaagcaa atcagctttt atcttcatca tgttacacca tgcaccggga aaatccctga
     9721 caattttctt ttaaagatta ctaactattt tttgacatac caaaatcaac tattttttgc
     9781 caaatgtcac tataattgac tataacaaac atttttttta ttggaaaatt tgaatcacta
     9841 tcttaatcaa actaaaaggt attttaaatg acacataatt atcagatttt tatcgcatta
     9901 tatgcatttt ttgttctatg ggctcttgtt ctggcgaata gaagatcatg catttttgta
     9961 aatataaaac ccataccggt cttattatgc tgtattcttc tcacttcaat cgtattaaac
    10021 atagaggtgc atattgctct ctacaaatat acggttccat tggcactttt tttcggatgt
    10081 gtgggtgact acctgataac ccgtctcgat gatttcagat acggcatgat actttttttt
    10141 gcgggacact gtatttatat tatctttttt atgggcatca cgggttcaaa ctttgaccat
    10201 agagcttttt tagccttatt gcctttagct atttatttac cattactgat aaggaaacta
    10261 cccacaggat ataaaaactt aagtttactg gtaacaacat acgcggtcac gcttggaata
    10321 atggtctgca ca
//
LOCUS       DAOTGZ010000081        10106 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_3064
            NODE_7923_length_10106_cov_8.409710, whole genome shotgun sequence.
ACCESSION   DAOTGZ010000081 DAOTGZ010000000
VERSION     DAOTGZ010000081.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868088
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 10106)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10106)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:22:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,785
            CDSs (total)                      :: 2,751
            Genes (coding)                    :: 2,732
            CDSs (with protein)               :: 2,732
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 12 of 19
            Pseudo Genes (internal stop)      :: 5 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10106
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7923_length_10106_cov_8.409710"
                     /isolate="AMR_MDS_3064"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..581)
                     /locus_tag="P5123_06880"
     CDS             complement(<1..581)
                     /locus_tag="P5123_06880"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024629.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 39 protein"
                     /protein_id="HRX16020.1"
                     /translation="MKKIYNIIKLFLESKKQYFVKTFTEDYSEDPAGFKSSSVNAPLS
                     KRQYIIWAALFLSGVFLTVFRSTHEAVWYDESYTIAAIGNSFGEMVKMIGRDSHPPLY
                     FLGLKLFTVCFGNSLFILRLFSALGIIFMGSLGFTHIRKLWSQKAALIYTLLCYTMPV
                     SIFQAQEIRMYSWCGAFVAWATILLYQAWQSGK"
     gene            complement(606..1457)
                     /locus_tag="P5123_06885"
     CDS             complement(606..1457)
                     /locus_tag="P5123_06885"
                     /inference="COORDINATES: protein motif:HMM:NF012738.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HRX16021.1"
                     /translation="MNGFNSITTKNSKIHDIYIYAFLVILTIICALPLYLMLIGSTLD
                     DRTIVNGFQFYPGSNFQNNMDLIDRAVPLWQGFSNSLKISIPYTLLAAYFSALTAYGF
                     SVYRFRFKKYLFGFVLATMMIPQQLGIIGYFDFCLKLGLVDTFFPLIITGTVNTLSIF
                     FIKQYADQVLHPSLIEAARIDQATELQIFHTIALPVLTPVVATMSIFNFVFSWNNLMA
                     PILLIFSDDKYPLSVLIASLSSGKYPVNLGATYLAITLSLMPIILIYTYCSKHIISGL
                     TMGSVKE"
     gene            complement(1736..1939)
                     /locus_tag="P5123_06890"
     CDS             complement(1736..1939)
                     /locus_tag="P5123_06890"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRX16022.1"
                     /translation="MKKNMNINEVKNAGYEALNKSLGSDGMIRFLKEIDSGHDDYTKK
                     RQKNIDKYSVDEIYNDITKNRAK"
     gene            complement(1936..2385)
                     /locus_tag="P5123_06895"
     CDS             complement(1936..2385)
                     /locus_tag="P5123_06895"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="PIN domain-containing protein"
                     /protein_id="HRX16023.1"
                     /translation="MLKIYLDACCYNRPFDDQSFDRIRLETEAIIYIFKKIREKKLKL
                     MGSEVLSYEINKISDPDRKYKIDFLLKNITKTIILNENIKQKALVFENIGFSGFDALH
                     IASAGIGKSDVFLTTDDKLLKLYKRNKSEIDIIVENPLIWITELLTI"
     gene            complement(2635..2979)
                     /locus_tag="P5123_06900"
     CDS             complement(2635..2979)
                     /locus_tag="P5123_06900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004784342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system HicB family
                     antitoxin"
                     /protein_id="HRX16024.1"
                     /translation="MKDILNYKDFIATVHFSSDDEIFFGKIEGINDLITFEGNSVADL
                     KEAFIDSVEEYLSLCKKTGKQPHKSYKGSFNIRISPLLHRKAAEKAMLKGISLNQLVQ
                     EVLEKELSSNTH"
     gene            complement(2976..3236)
                     /locus_tag="P5123_06905"
     CDS             complement(2976..3236)
                     /locus_tag="P5123_06905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014904207.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system HicA family
                     toxin"
                     /protein_id="HRX16025.1"
                     /translation="MSKHMKLLNRFLSKPKDFTYNELKTLLSGFDYIELTKGSTSGSR
                     ISFYNQNVDHIICLHKPHPKNILKFYQIELIIEELKSKEIIL"
     gene            complement(3508..3702)
                     /locus_tag="P5123_06910"
     CDS             complement(3508..3702)
                     /locus_tag="P5123_06910"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRX16026.1"
                     /translation="MKQAKYMSENEVLEKGVTALLSELGPVETPRFLNISRDKCIESV
                     KRHREWQKNHDKDNFFSDVF"
     gene            complement(4020..4529)
                     /locus_tag="P5123_06915"
     CDS             complement(4020..4529)
                     /locus_tag="P5123_06915"
                     /inference="COORDINATES: protein motif:HMM:NF024811.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD hydrolase-like protein"
                     /protein_id="HRX16027.1"
                     /translation="MVDADDTILDYKQAEINAINKVFNHINMECNIKAGEVFGDICKE
                     LWSLYKLDNVNDEIVQAQYHSLYYEYQIERFRKLKMEFDFDILAEELSELYLNSFAEE
                     TVAICHAEEVCAKISAVCKLIIITNGLTRIQLSRLGMFKPYIYKTYVSEEIGFINQVT
                     DTSTMLSTI"
     gene            complement(4715..5047)
                     /locus_tag="P5123_06920"
     CDS             complement(4715..5047)
                     /locus_tag="P5123_06920"
                     /inference="COORDINATES: protein motif:HMM:NF024835.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HRX16028.1"
                     /translation="MNKKINKDTTTFDEFMSDPVQKQKFDKDYSNFLLKEFLHEAMNE
                     NHISVRKLAEQSGISTSIIQNIRSEKATNVTFYTLSSLMSTLGYKINFQKIGKDRKKN
                     EKKYSKTK"
     gene            complement(5040..5426)
                     /locus_tag="P5123_06925"
     CDS             complement(5040..5426)
                     /locus_tag="P5123_06925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006869000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HRX16029.1"
                     /translation="MNISNRVAYIGESFTIEWYYDEKGFSQAFDYYSTLSDLQKRKVL
                     LLFKRIGDYGKINDITKFRYEGDQIFAFKPKPNRFLSFFVKDKKIIITNAFTKKTNKL
                     PKKEKAIAIEKRDIYLERNMKGTYYE"
     gene            complement(5624..5818)
                     /locus_tag="P5123_06930"
     CDS             complement(5624..5818)
                     /locus_tag="P5123_06930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011745530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRX16030.1"
                     /translation="MKQAKYMSENEVLEKGVTALLSELGPVETARFLNISRDKRIESV
                     KRHREWQKNLDKDAFFNDVF"
     gene            complement(5815..6261)
                     /locus_tag="P5123_06935"
     CDS             complement(5815..6261)
                     /locus_tag="P5123_06935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008868329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRX16031.1"
                     /translation="MMKKIYLDVCSLCRPFDDQNYIRIRLETDAVNLILTKVRSNIYQ
                     LMKSKVHEFEIRHISDKYEQIELLGLLDKYGNNISIDPKKLRTRAEELVSFNYGIADA
                     AHVAYAESFESDFISCDDKLIRKCEKHVTLINCYNPVSFCKMENLK"
     gene            complement(6348..8651)
                     /locus_tag="P5123_06940"
     CDS             complement(6348..8651)
                     /locus_tag="P5123_06940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012035282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrA"
                     /protein_id="HRX16032.1"
                     /translation="MPVQTAEKENQANAGCRTRNIEVINTRVHNLKNLSVTIPRNQLV
                     AFTGVSGSGKSSLVYDTIYAESRRQLLETFSSYARNRMPKISRPDADGIFNISTPIVI
                     NQRPMGTNPRSTVGTATDIYSFVRLLYSRFDDRQNYFPASAFSFNDVEGMCPACRGLG
                     IESVLDANMLVDYNLSVKEGAIKHPMFAIGKWFWKALTMCGFFDPDKPVKEFTDQELH
                     MLLYSQETKFSCERMGEKYESKYTGVITKIKERHERGTSNPAGFESYIKQQTCSDCGG
                     SRLNRQVRDITVYGVPLNKLVLMEMPELYNFINSIEIEEAQPIVSRISSAVQNLIEIG
                     AGYLHLNRSVGTLSGGESQRVKMARQLDCDLINVMYILDEPTSGLHPKDVDSVISMLK
                     RLRDRENSVLVVEHDPEVILSADTIVEIGPGAGSKGGELVFAGSKESFLRQKSSTAIE
                     LKKKISIGKKRTSNTQPFVIRNASGNNLKNISVDIPSGVLVAVTGPAGSGKSTLIHNH
                     FMNNYPDTICVDQGSPFRTNRSNAMSYTKIFDAIRAEFARATNSDPGLFSFNSKGGCP
                     ECNGQGQVKIEMSFLEEVTTTCKACNGKRYIDSVLQIKYKGKNIYDVLCMTALDARDY
                     FEDPAIKHGLDMLITVGLDYIELGQSMSTLSGGELQRLKLAKELKHHGNTYVLDEPTS
                     GLHLKDIDRLLGILQSLVNNGNSVIVIEHNLQVIANADWIIDLGPEGGKNGGRIIAQG
                     TPDTLCEDKDSVTGQYLKRFLKGSDSV"
     gene            complement(8705..9673)
                     /locus_tag="P5123_06945"
     CDS             complement(8705..9673)
                     /locus_tag="P5123_06945"
                     /inference="COORDINATES: protein motif:HMM:NF018184.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HRX16033.1"
                     /translation="MNRTDNRIRYIAKINRAIDFIDANLDQELTLDQVARFAGFSEFH
                     FHRIFSNIVGETFGRFVQRVRVERSAMMLTNDTRKSITDVAYACGFSSSASYSRLFRE
                     FFACTPSQWREGEAQKSKFRILQGKDYHIQSTPRKDINIRSLYNQSMNSFEWKIMLKG
                     EEFSQIRIETHPEREIAYIRHIGPYFNDEALFCSLFEKLFRWAVPRKLITDKTRNYCI
                     YHDDPAITEPEKLRLSVGIDIPAQTEISGEVGRLVLPAGEYAMAKLELSSDQYPLAWN
                     IIYRDWLPESGYMCGDGLPYETMLQDPAEHPEGKHVFEICIPVVPL"
     gene            9970..>10106
                     /locus_tag="P5123_06950"
     CDS             9970..>10106
                     /locus_tag="P5123_06950"
                     /inference="COORDINATES: protein motif:HMM:NF019884.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH domain-containing protein"
                     /protein_id="HRX16034.1"
                     /translation="MKAERFFTILNILLNKRKISAPKLARHVNVSVRTIYRDIDALSL
                     AG"
BASE COUNT         3036 a         1908 c         1818 g         3344 t
ORIGIN      
        1 aattttccac tctgccatgc ctgatacagt aaaatggttg cccatgcaac aaaagcgccg
       61 caccagctgt acatacggat ttcctgtgcc tgaaaaatgg agaccggcat tgtataacag
      121 agcagagtgt agataagagc tgctttttgt gaccagagtt tacgaatatg tgtaaatcca
      181 agcgatccca taaatattat tcccaaagca gaaaaaagcc gtaatataaa taacgaatta
      241 ccgaaacaaa ccgtaaaaag tttcaaccct aaaaaataga gaggggggtg gctgtcgcgt
      301 cctatcattt tcaccatttc accaaagctg tttccaatag ctgctatggt ataggactca
      361 tcataccaga ccgcctcatg agttgaacgg aaaactgtta aaaaaacacc tgaaaggaaa
      421 agtgctgccc agattatata ttggcgtttt gataagggtg cgtttacaga gctgctttta
      481 aaacctgccg gatcttcact gtaatcttcc gtaaacgttt taacaaaata ttgctttttt
      541 gattctaaaa acaatttgat tatattgtat atttttttca tatagttatt ttctcctgac
      601 agtgattact ctttaactga tcccattgtt aagccgctga ttatatgttt tgaacaatag
      661 gtataaatca gaattatcgg cattaatgat aacgtaattg ccaggtacgt tgcgccgaga
      721 tttaccggat attttccgct gcttaatgaa gcaatcagca ctgaaagcgg atatttatca
      781 tcggaaaata tcagtaatat gggcgccatt aagttattcc agctgaatac aaaattaaat
      841 attgacatag tcgctaccac cggtgttaaa acggggaggg ctattgtgtg aaagatctgc
      901 agctccgtag cctgatctat acgggcagct tctatcaacg agggatgcag tacctgatca
      961 gcatactgtt ttataaaaaa tattgaaagg gtatttaccg taccggtgat aataagcggg
     1021 aaaaaggtgt ctacaagacc caacttcaga caaaaatcaa aataaccgat aatacctaat
     1081 tgctgcggaa tcatcatggt tgctaaaaca aaaccaaaaa gatatttttt aaatctgaat
     1141 ctatagacag aaaaaccata tgcagttaaa gcggaaaaat atgctgctaa cagtgtatac
     1201 ggaatagaaa ttttcaggct gttggaaaac ccctgccata aaggaacagc tctatctatc
     1261 aaatccatat tattttgaaa gttggatccc ggataaaact gaaacccgtt tacaattgtt
     1321 cgatcatcaa gtgtactgcc gatcagcatc agatagaggg gcagcgcaca aataattgtt
     1381 aaaataacca aaaaagcata aatatagata tcatgaattt ttgagttttt tgttgtaatc
     1441 gaattaaatc cattcattga gagtccttca atttctaatt tgtcacgaaa ttgaatttaa
     1501 ttctgatgat gattttgtaa acagttaaac tctaatgtag gtcaattgca atggaactaa
     1561 agtactaaat actaaaatca aaaataaaaa agagtatttt tactcaaagt gagggattct
     1621 tatttgctat cactgtggga ttgctgtttg ccgtttgcat tttgtccgaa gaccggaacc
     1681 cagcgctact gggtgaggac cggagctgtt atgatttatt gtctgacgtt tttgatcact
     1741 tagcacgatt ttttgttata tcgttataaa tttcgtctac agaatactta tctatatttt
     1801 tttgacgctt tttagtatag tcatcatggc cagaatcaat ttcttttaaa aatcgaatca
     1861 ttccatcaga acctagtgat ttatttagcg cttcataccc agcatttttt acttcattaa
     1921 tattcatatt ctttttcata tcgttaataa ctccgtaatc catattaaag gattttcaac
     1981 aatgatatca atttctgatt tatttctttt atataacttc aataatttat catcagttgt
     2041 taaaaaaaca tcactttttc ctattccagc agatgctatg tgtaaagcat caaagccaga
     2101 aaacccaata ttttcgaaaa ccaatgcttt ttgcttaata ttttcattca aaatgattgt
     2161 tttcgttata ttcttcaata agaaatcaat tttatatttt ctatctggat cagaaatttt
     2221 attaatttcg taagataaaa cttcactacc catcaatttt agtttttttt ctctgatttt
     2281 cttgaaaata taaattatag cctcagtttc tagccgtatc ctatcaaaag attggtcatc
     2341 aaatgggcga ttatagcaac aagcatctaa ataaattttt agcattaaaa ctccaaaata
     2401 ttttttaaat tgatataata tatcagttta aaaaatcaag tattaatatt gattacagca
     2461 gatgaggtaa gaagtaaatt taggttataa aaaaatacag aaaaacgtga tcaaaaattt
     2521 tgaacaccgt acgaacgttg acgacggcct tatcgccgca atgtgccgaa gggcgcagcg
     2581 agtctttccg cgagcgccga agcaactctc agttgtgcgt agttggcgca aatctcagtg
     2641 tgtatttgat gataattctt tttcaagaac ctcttggact aattgattta aagaaatacc
     2701 ttttaacatt gctttttctg ctgcttttct gtgtaataaa ggagaaatcc taatattgaa
     2761 acttccttta tatgatttat gaggctgttt acctgttttt ttacataatg atagatattc
     2821 ctcaacagaa tctatgaaag cttctttcaa atcagcaaca gaattacctt caaatgtgat
     2881 aagatcattg attccttcaa ttttaccaaa aaatatttca tcatcagagc taaagtgtac
     2941 agtagctata aagtctttat aatttaatat atctttcata agattatctc cttacttttt
     3001 aattcttcaa taatcaattc aatttggtaa aattttagaa tattcttggg atgaggcttg
     3061 tgaagacaaa taatatggtc aacattttga ttataaaagg aaattcgtga tccagaagtt
     3121 gaaccttttg taagttctat ataatcaaat ccagatagaa gtgttttaag ttcattgtag
     3181 gtgaaatctt taggcttcga taaaaatcgg ttcaataatt tcatatgttt actcatgagg
     3241 gtaatataag gatacaacat agaattgcaa ctataattta gttacaattt tgaattttta
     3301 ttacttgtca ttttcaaaat gaagcttaaa attcatcaag aaattatatt ctattcattt
     3361 tgtgggtttt gcgccaatta cgaacaacta aagtatatcc gccatattac gtatatacac
     3421 ccctattcag aaatcacatg cagtatttat cccctaacct aatcctattc aaatcaaaat
     3481 aaagtacaat aataaacagt cttaacacta aaacacatca ctaaaaaaat tgtctttatc
     3541 atgatttttt tgccattcgc gatgcctttt aacagattcg atacacttgt ctctggaaat
     3601 atttaaaaat cggggtgttt ctactggtcc aagctcagac aataatgcag taactccctt
     3661 ttcaagaact tcattttcac tcatatattt tgcttgtttc attttaaatt ctccatttta
     3721 caaaaagata caggattaag ataatttatc aaccaggttt tggcatgttg aagaggcagt
     3781 gttaggggtg ttgtaaacgg aaagtatcag tattctggcg tttttcgggt tatattacta
     3841 taaaataaac ctcaatacat ctcgtaaatc attaatctca tatgttggat taattaatgt
     3901 catgttctgc ttactctttg gattatacca acagctatct attcctatat tcttggcacc
     3961 cttgatgtct gatgataatg aatcgcctat cacaacaact ttggaaatat cagttatgtc
     4021 taaatcgttg ataacatagt tgaagtatcg gtgacttggt taataaaacc aatctcctct
     4081 gatacgtatg ttttatatat gtatggctta aacattccta atcgagataa ctgaattctt
     4141 gttaatccat ttgtgataat aatcaactta catacagcac taattttagc acatacttct
     4201 tctgcatgac aaatagccac tgtttcttct gcgaaagaat taagatataa ttcgctcaat
     4261 tcttctgcta gaatatcaaa atcaaattcc atttttaact ttctaaaacg ttcaatctgg
     4321 tactcataat ataatgaatg atattgtgct tgaactattt catcgttgac attatcgagt
     4381 ttatataggg accacaattc tttgcatata tccccaaata cttctcccgc cttaatatta
     4441 cattccatat ttatgtgatt gaaaacttta ttgattgcat taatttccgc ttgtttatag
     4501 tccaatatag tatcgtctgc atcgaccata aaatattcgt atctcaccat atttttcctt
     4561 tattcaaata attaataacc tttgatatgg tagtgttcaa tgtcatttaa cgttttcgtc
     4621 gtttgcgatg ttttgccgag cgtaagagag gtaaaacgtg gtaaagttca agagaccgga
     4681 gggagcacct cgctttacac tgtgtttggc gacgttattt tgttttggaa tatttttttt
     4741 cgtttttttt tctgtctttt ccgattttct ggaaattaat tttatatcca agagttgaca
     4801 ttaatgagga tagagtataa aaggttacat tagttgcttt ttctgatctg atgttttgaa
     4861 taatactggt tgatataccc gattgttctg ctaactttcg tactgaaata tggttttcat
     4921 tcattgcttc atgcaaaaat tcttttaata agaagttact gtaatcctta tcaaattttt
     4981 gtttttgaac aggatcactc ataaactcat caaatgttgt agtatcttta ttaatctttt
     5041 tattcataat atgttccttt catatttctt tcaagataaa tatctctttt ttcaatggct
     5101 atcgcttttt ctttttttgg taacttatta gtttttttag taaaagcatt ggtaatgata
     5161 attttcttgt cttttacaaa gaaagataaa aacctattag gttttggctt aaatgcaaaa
     5221 atctgatccc cttcataccg gaattttgtt atatcattta ttttaccata atctccaatt
     5281 ctcttgaaaa gaagtaaaac cttacgtttt tgaagatcag ataatgtaga ataataatca
     5341 aatgcttggc taaaaccttt ttcatcataa taccattcaa tagtaaaaga ttctccaata
     5401 tatgcaacac gattagatat attcatgctt taatgtatca acatatagat acgctgtcaa
     5461 ctaaaatttg gcggcatttt tataggttta gcataatttc taaaaattac gaacacggtt
     5521 tgttcaaaat aatgtagcac aactaaagta tatccgctat atttttatct caacctaatc
     5581 tttttcaaat caaaataaat tacaataata atcagactta acactaaaac acatcgttaa
     5641 aaaaagcgtc tttatccaga tttttttgcc attcgcgatg ccgtttaaca gattcgatac
     5701 gcttgtctct ggaaatattt agaaatcgag ctgtttcgac tggtccaagt tcagacaata
     5761 atgcagtaac tcccttttca agaacttcat tctcactcat atattttgct tgtttcattt
     5821 taaattctcc attttacaga aagatacagg attataacaa tttattaaag taacatgttt
     5881 ttcacacttt ctaattagtt tatcgtcaca gctaataaaa tctgattcaa aagattcagc
     5941 ataagcaaca tgagccgcat cggcaatgcc ataattaaaa ctgaccaact cttctgcccg
     6001 tgttctaagt ttttttgggt caattgagat gttgttacca tatttatcaa gtaagcccaa
     6061 aagttcaatt tgttcatatt tatctgaaat gtgtcgaatc tcaaattcat gaacttttga
     6121 cttcattaat tgatatatgt tacttcttac ttttgttaaa attagattta cagcatctgt
     6181 ttctaaacga atacgaatat aattttggtc atcaaatggt ctgcaaagcg aacaaacatc
     6241 caaataaatc tttttcatca tactagtttc ctttaaaata tttatataat gaacagtatt
     6301 attagaaaaa gatcaatact tttttattca atgttccatc aactccccta tacagaatcc
     6361 gaccctttca aaaatctttt cagatactgc ccggttacgg aatctttgtc ttcgcacaaa
     6421 gtgtccggtg ttccttgcgc gataatgcgg ccgccgtttt tgccgccttc ggggccaaga
     6481 tcgataatcc agtcggcgtt ggcaattacc tgcaggttgt gttcgataac aattacagag
     6541 tttccgttat ttacaagcga ctgcagtatg ccaagaagac ggtctatgtc tttaaggtga
     6601 agtcccgatg tcggctcgtc cagcacgtag gtatttccat ggtgcttcag ttctttagca
     6661 agtttaagcc gttgaagttc gccacctgac agggtagaca tcgactggcc cagttcgata
     6721 taatcaagcc ccaccgttat taacatatca aggccgtgct ttatagccgg atcttcaaaa
     6781 tagtcgcgcg cgtcaagcgc ggtcatacag agtacgtcgt agatgttttt gcccttgtat
     6841 ttaatctgaa gaaccgaatc gatatagcgt ttaccgttac aagccttaca ggttgtagtc
     6901 acctcttcaa gaaagctcat ctcaatctta acttgaccct ggccgttaca ttcggggcat
     6961 ccaccttttg agttaaagct aaaaagcccc gggtcgctgt tggtcgcgcg ggcaaactcg
     7021 gcgcgtattg cgtcgaaaat ttttgtgtaa gacatggcgt ttgagcggtt tgttctaaag
     7081 ggcgagccct gatccacgca gatggtgtcc ggatagttat tcataaaatg attatgaata
     7141 agagtgctct tgccgcttcc ggcaggacca gtaacggcta caagtactcc cgatggaata
     7201 tccacagata tattcttcag attattgccg gacgcgtttc tgataacaaa gggctgggta
     7261 ttgcttgttc gttttttacc aatggatatc ttttttttaa gctcgattgc cgtagagctc
     7321 ttttgtctta ggaagctctc ttttgaaccg gcaaaaacaa gttcgccacc tttggatccg
     7381 gcgccggggc cgatctcgac aatggtatcg gccgagagta taacttcggg atcatgctcc
     7441 acaaccagta cactgttttc tctgtcgcga agccgtttaa gcattgatat aacagagtct
     7501 acatccttgg gatgcagccc ggaggttggt tcgtcaagaa tgtacataac gttaataaga
     7561 tcacaatcaa gctggcgtgc catctttaca cgttgcgatt cgccaccgga gagagttccg
     7621 accgatcggt tcaggtgcag ataaccggct ccgatttcta taaggttttg taccgccgaa
     7681 gagatacggg agactatcgg ctgtgcctct tcaatttcaa tgctgttgat gaagttgtaa
     7741 agctccggca tctccataag aaccagtttg tttaagggta ctccataaac ggtgatgtca
     7801 cgaacctgtc tgttaagtct tgatcctccg caatcggagc atgtctgttg tttaatgtag
     7861 ctttcaaaac cagcagggtt tgaagtgccg cgttcatgtc gttctttgat ttttgttatt
     7921 acaccggtat acttcgattc atatttttct cccattcgct cgcagctaaa ttttgtctct
     7981 tgggaataga gcagcatgtg aagttcttgg tcggtaaatt ctttaactgg tttatcagga
     8041 tcaaaaaacc cgcacattgt tagggccttc cagaaccatt ttccgattgc gaacatggga
     8101 tgttttattg ctccctcttt gactgaaaga ttgtaatcga caagcatgtt tgcatcaagt
     8161 accgattcga ttccaaggcc gcgacatgca gggcacatgc cttctacatc gttaaaagag
     8221 aaggcgctgg caggaaaata gttttgccta tcgtcaaagc gcgaatagag aagccgtaca
     8281 aaggagtaga tgtcggttgc tgttcccaca gttgatcggg gatttgttcc cattgggcgc
     8341 tgattgataa ctatgggagt agaaatatta aagatgccat ccgcgtcggg gcgcgaaatt
     8401 tttggcatgc ggtttcttgc ataagagctg aatgtttcaa ggagctgtcg tcttgattcg
     8461 gcatagatgg tatcatacac cagtgatgat tttccgcttc cggaaacacc ggtaaaggca
     8521 accagttggt ttctgggtat ggttacggaa agatttttca gattatgaac tcgagtgtta
     8581 attacttcga tattacgagt tcgacagccg gcgttggcct ggttttcttt ttcagctgtt
     8641 tgtactggca ttgttccacc tctttgttat tggttggaac agtcgtaaga acgaaagatt
     8701 ctgtttacag gggtacaacg ggaatacaga tttcgaaaac gtgcttgcct tcgggatgct
     8761 ccgccgggtc ttgaagcatg gtttcgtaag gcagtccgtc gccgcacatg tatccgcttt
     8821 cgggcagcca atccctgtag ataatgttcc acgcaagcgg atattggtca gaactgagct
     8881 caagcttcgc catggcatac tctccagcgg gtaaaaccaa ccggccaact tcgcccgata
     8941 tttccgtttg tgctgggata tcgataccca cgcttaaacg caacttctcc ggttcggtaa
     9001 tggccgggtc atcgtggtag atgcagtagt tgcgcgtttt atctgtaatc agtttgcgag
     9061 gcaccgccca gcggaaaagc ttttcgaaaa gcgaacagaa cagagcctca tcgttaaagt
     9121 agggcccaat atgtctgata taggctatct ctctttccgg gtgcgtttca atccgtattt
     9181 gtgaaaactc ctctcccttt aacataatct tccattcaaa actgttcatg ctttgattat
     9241 agagggatct gatgtttatg tctttccggg gagtgctctg tatatgataa tccttgccct
     9301 gtaatatgcg aaacttgctt ttttgagctt cgccttcgcg ccactggctt ggggtgcagg
     9361 cgaaaaattc tctaaacaga cgtgagtaag atgccgagct agaaaaaccg caggcatagg
     9421 cgacatctgt tatcgatttt cttgtgtcgt tggttaacat cattgccgaa cgctctaccc
     9481 ttacgcgctg aacaaaccgg ccaaaggttt ctccgacgat attcgagaat attcggtgaa
     9541 agtgaaactc agaaaatccg gcaaagcgcg cgacctgatc aagggttagc tcctgatcca
     9601 gattagcatc gataaaatcg atcgcgcgat taatttttgc tatgtaacga attcggttat
     9661 ctgtacggtt caatggatat cctcgatttg tatgataact gaatatattt gcatgcgtta
     9721 attgagtaaa gtaaattatc ggtattgtta acggggacgg tcgtaattca gtatagggtt
     9781 ttgtagacat ttgtctttgt agctggctaa caattacgaa aaacagctgt actttgaatg
     9841 gaaagtggct aacaatccag aaattcagct gagactggct aacaatccgg gtattcgtac
     9901 gttcacataa atttttaata tcaaaacggc actgtcccca ttaattcaat aaaaagcagg
     9961 aaataaaccg tgaaagcaga acgattcttt acaattctta atatacttct taataaaaga
    10021 aagatctccg ctcccaagct cgcgcggcac gtcaatgttt ctgtaagaac aatataccgg
    10081 gatattgacg cattgtcttt ggctgg
//
LOCUS       DAOTGZ010000082        10095 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_3064
            NODE_7936_length_10095_cov_5.868227, whole genome shotgun sequence.
ACCESSION   DAOTGZ010000082 DAOTGZ010000000
VERSION     DAOTGZ010000082.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868088
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 10095)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10095)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:22:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,785
            CDSs (total)                      :: 2,751
            Genes (coding)                    :: 2,732
            CDSs (with protein)               :: 2,732
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 12 of 19
            Pseudo Genes (internal stop)      :: 5 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10095
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7936_length_10095_cov_5.868227"
                     /isolate="AMR_MDS_3064"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(115..1509)
                     /locus_tag="P5123_06955"
     CDS             complement(115..1509)
                     /locus_tag="P5123_06955"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRX16035.1"
                     /translation="MKQIRIIMCAAIVLFSAEILPVEYDEGYLLETPESYYGRIEYLP
                     TLKKINYIRGAVVRNRDVFISWIKLEEKDVVYTIYRNTRVMDTYMAFESAIAVGEVTD
                     RESFLDTSIRESGVYYYAVTTKKSGYAEDKNLKAYMSYLPDGFTVRTGVDHSTVYSID
                     AKRYSDGIVISWKNPYNYKGNLFVYRSTDVIDSSEALNKAVMIKKTDNVETYRDRNAS
                     RNFYYAVLMEGPDNRPARIFSPRNNFTGRSSTFSTELPSNGNARQYLNASIEGGRAAL
                     SWRIPRGKYLLFKSSTRPETRRDARRGQLLKRFDSSLNYYYDNSGDSNSFYSIVPENR
                     ADDTRMLVEGIDMVFLSSNKAVEPKIKSGTEVSRLDDVTSSDVSADSIDRLVKEFYRT
                     KNYKPIIRQLLKIASGSSNEPYRAKAYFFAGRIIAEQGDYRMALNYLYRDDVLKYYPK
                     EAKFWQDYCLTKLE"
     gene            1743..3008
                     /gene="murA"
                     /locus_tag="P5123_06960"
     CDS             1743..3008
                     /gene="murA"
                     /locus_tag="P5123_06960"
                     /EC_number="2.5.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012402131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008760 - UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase"
                     /protein_id="HRX16036.1"
                     /translation="MDIYKINGGKKISGDIPISGAKNAALPIIVGSLLADGTTKLTNV
                     PDLMDIRTTIKLIEHLGAKTSFDKDTNTLTIDAKEIINGEVPYDLVKTMRASIYVMGP
                     LLARCQEADVSLPGGCAIGARPVDIHLQGFEKLGTDINLDHGYVKARTKGLKGALYVM
                     PKVSVGATINLIMGAVLADGETVLENCAMEPDVVDLGNFLIKMGADIQGLGTERIAVK
                     GVSSLKAVEYHIMPDRIEAGSYLLAGAAVRGNIKVTDIIPDHIEALLDSLKEMGFQIK
                     TGSNWASVAPSDNLRGILVETTPHPGFPTDLQAPMMALMTTVPGISVMIEHIFENRFT
                     HVAELHRMGAQITIEGKCAIIKGGTSLMSAPVMMSDLRAGAALVIAALAAEGETEIRR
                     IYHCDRGYEKLCHKLSAVNADIRRVTGGM"
     gene            3009..3839
                     /locus_tag="P5123_06965"
     CDS             3009..3839
                     /locus_tag="P5123_06965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004420834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook-basal body protein"
                     /protein_id="HRX16037.1"
                     /translation="MIRGIYTGASGMISQQAKMDVTANNLANVDKTAFKKDTTIFKAF
                     PDMIIRRINDDGVGIVPTGSYDSMPYVGKLGTGVEVNEVFTQFEQGSLQRTENQFDLA
                     LEGEGFFTVMTEKGERHIRNGSFTINDEGILMTHNGNPVMGENGIIRIQKHNFVVREN
                     GEIVVNRDLLEEPVVGQNENEWQDQVVIDKLKIVDFEYKRHLKKEGTSMYRETEESGP
                     ALPPLNLKIHQGFLEKSNVNVVRGMVDMIEVQRSYEANQKSIQTADQTLGKLISEVGR
                     "
     gene            complement(3910..5130)
                     /gene="hydF"
                     /locus_tag="P5123_06970"
     CDS             complement(3910..5130)
                     /gene="hydF"
                     /locus_tag="P5123_06970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011698698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="[FeFe] hydrogenase H-cluster maturation GTPase
                     HydF"
                     /protein_id="HRX16038.1"
                     /translation="MQNTPKGNRLHIGIFGRRNVGKSSLLNLMVRQQVSIVSDFAGTT
                     TDPVEKPMELLPLGPVLFIDTAGIDDIGELGDLRVKKTTLVFKRTDLAVIVTDGTWGD
                     FEERICQECAKYAIKFIVVFNKSDEVKPDNALIEKLAEEEIPFVHTSALKNSGIKDFR
                     QALLDNAPPEFLDNPQIIGDLINPGETVVLVVPIDKEAPKGRLILPQVQTIRDLLDSD
                     SICVVAKERELKAAIDNLKTPPKLVVTDSQAFLKADADTPKDILLTSFSILYSRFKGD
                     LAIQTQGAIAVENLKPGDRVLIAEACSHHPITDDIGTVKIPRWLNQYVGGELDFSNMQ
                     GRDFPEDLSEFKLVIHCGACMWNRREMLSRMLQCKEAGVPITNYGLVIAYSLGIFKRA
                     LQPFPEALEVFLNR"
     gene            complement(5142..6536)
                     /locus_tag="P5123_06975"
     CDS             complement(5142..6536)
                     /locus_tag="P5123_06975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018606043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate ammonia-lyase"
                     /protein_id="HRX16039.1"
                     /translation="MDNNYRTESDSLGSLEILADSLFGINTMRALNNFPFKSRPAPES
                     LIRAYGTVKHAALLMCRDLDVWDNFKAESLINAAREMSQGELSSWIVTDMMQGGAGTS
                     LNMNVNEVIANRALILMGKCAGDYFTVSPTDDVNCFQSTNDSFPTALRLALINSLHSL
                     ESSVSGLQQSFENKEKEFAKIVKLGRTELREAVLTTLGREMGTYAEAFGRDRWRLYKC
                     EERLRVVNLGGTAIGTGLGAPREYIFGVVTKLRDLTGIGFARAENLQEATSNTDIFAE
                     VSGILKACAVNLLKIAGDLRLLSGPLGEINLVPVQTGSSIMPGKYNPVIAESVSQAAY
                     QVMSNDSTVTHLCAAGNLELNAFMPLLAYNMLDSAELLTESCKILALCVAGISANEQV
                     CQGQVENSTAVVTALVSALGYDTCSKIAQKSAESGKSIRDVSVGEGYLSSGEFDKLVS
                     FEAVCRLGSKIENI"
     gene            complement(6582..7961)
                     /gene="hydG"
                     /locus_tag="P5123_06980"
     CDS             complement(6582..7961)
                     /gene="hydG"
                     /locus_tag="P5123_06980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012625131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="[FeFe] hydrogenase H-cluster radical SAM
                     maturase HydG"
                     /protein_id="HRX16040.1"
                     /translation="MSIINAQNITEILNNGSCEKNRVKDILLKAKELKGLSIDEVACL
                     TSINDSERLEELFATAQFIKNTIYGRRLVIFAPLYISNLCANECLYCAFRAGNKSVHR
                     RALSQTEIANEIRILEKQGHKRVLLVAGESYPKEGFQYVLDSIETIYKTKNGHDEIRR
                     VNVNIAPLDLDQFRQLKKSAIGTYQLFQETYDRDVYSKVHTAGKKCDYDWRLTAMDRA
                     MEAGIDDVGIGVLFGLADWRFEILALMQHINYLDKNFGVGPHTISVPRLEPATGSEIS
                     EHPPVPVDDLTFKKIVAILRCAVPYTGIIMSTRETPKMREDTFALGVSQISAGSRTNP
                     GGYAEGADVADEAQFSLGDHRTLDEVIADVARLGYVPSFCTGCYRLGRTGGDFMDLAK
                     PGLIRHHCDPNAVSTFQEYLDDYASPETKDRGEALIEKVISEMDEQPRELSIKMVDKV
                     KSGKRDVFC"
     gene            8249..9502
                     /locus_tag="P5123_06985"
     CDS             8249..9502
                     /locus_tag="P5123_06985"
                     /EC_number="2.7.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005034999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008776 - acetate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006085 - acetyl-CoA biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetate kinase"
                     /protein_id="HRX16041.1"
                     /translation="MNSGEQSQKTEALVLVINCGSSSLKYEVYDMPQMISRGKGKVER
                     IGMETGNIEQKCENGVYTKETPVPDHKTAFKYVVEALTDIEKGIIKNISDITAIGHRV
                     VHGGESYSESVVIDESVLKAIEEYSELAPLHNPANLTGIIEAQNQFKGIHNVAVFDTA
                     FHQTMPPAAYLYGIPRELYRKYKIRRYGFHGTSHRYVANRALDLLYRSSVNTNLITCH
                     LGNGASITAIERGRSVDTSMGFTPLEGVMMGTRSGDLDPSILLFLMDRGFSSAELSEM
                     LNKKSGMMGVSEVSSDMRDLKEAARKGNQQAIETLEVYTYKIRKYIGSYAANLVKVDA
                     IVFTGGIGENNADIREQICQRLENIGIVLDKDKNNSIPKGGEGIISSDYSQAVILTIP
                     TNEELQIAVDTYKLVFNEELSSFQS"
     gene            complement(9701..>10095)
                     /locus_tag="P5123_06990"
     CDS             complement(9701..>10095)
                     /locus_tag="P5123_06990"
                     /inference="COORDINATES: protein motif:HMM:NF013933.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="LysE family transporter"
                     /protein_id="HRX16042.1"
                     /translation="SAVAGVAVVDYLYIMLSIIGVGALLENPTIKKIMLFISSAVLIV
                     FGVMLINKGINFVLSEASVATPESYIKSFVAVFLLTISSPLTIVFWTGLFSNKAIEYK
                     LNKKELLIFGAAAGLATIAFMGVSVIII"
BASE COUNT         3061 a         2295 c         1932 g         2807 t
ORIGIN      
        1 cttagcggcc tcgttttctg gtctaaaagt tagctcataa taggttatca tcaatagacc
       61 ggctgttatc agcagcgatg cccctatcac tcttttcgcg ttcataaata atacctattc
      121 caattttgtc agacaatagt cttgccagaa ctttgcctct ttaggataat acttaagtac
      181 atcatcacgg tacaaataat ttaatgccat acggtaatct ccctgttcgg caataatgcg
      241 accagcaaaa aaataggcct tggctctgta aggctcgtta ctactgccag atgctatttt
      301 aagaagctgt cgtataatcg gcttatagtt ctttgtacgg taaaattcct taaccagccg
      361 gtcaatgctg tcagccgaaa catctgaact tgttacatca tcaagcctcg atacctccgt
      421 gccgcttttt atcttaggtt ctacagcttt attagacgaa aggaaaacca tgtctattcc
      481 ttcaacaagc attcgtgtgt catcggcacg attttccggt actatcgaat agaaactatt
      541 tgaatcccca ctattatcat agtaataatt caacgaacta tcaaatcttt ttaataattg
      601 tccgcggcgc gcgtctcgac gggtttccgg tcttgtgcta cttttaaaaa gaagatattt
      661 gcctcttggt atccgccagc ttaatgcagc tcgccctcct tcaattgacg catttaaata
      721 ttgacgtgcg tttccatttg atggcagttc ggtactgaaa gtcgagcttc gacccgtaaa
      781 attatttctg gggctaaaaa ttcgtgccgg tcggttatcc ggcccctcca ttaaaacagc
      841 atagtaaaaa tttcttgaag cgttacgatc ccgataggtt tcgacattat ccgtcttttt
      901 tatcattaca gccttgttca acgcttcact agaatcaatt acatctgtcg aacggtaaac
      961 aaacagattt cctttgtagt tataggggtt tttccagctg ataacaattc cgtcactgta
     1021 acgcttggca tcaattgaat atactgtaga gtgatcaaca ccggttctaa cagtaaaacc
     1081 atcaggtaaa tagctcatat acgccttaag gtttttatcc tcggcatatc cgctcttttt
     1141 tgtcgttaca gcataataat aaacacctga ctctctaata gaagtatcaa gaaaactctc
     1201 cctgtcggtg acctctccta cagcgattgc gctctcaaag gccatatatg tatccataac
     1261 acgcgtatta cggtaaatgg tataaacaac atccttttcc tcaagtttga tccagcttat
     1321 aaaaacatcc cgattacgta caacagcgcc ccggatatag ttgatttttt tcagggttgg
     1381 caaatactca attcgtccat aatacgattc aggcgtctcc agcagataac cctcatcata
     1441 ttctacgggc aaaatttctg ccgaaaagag cacaattgca gcgcacatta tgattctaat
     1501 ctgtttcata tctcatttaa atatccatat tagatcagcc tgttttaaaa cttgtaaaaa
     1561 aaaacagaac cccatttcat tttcggtaat taatagcatt atctttaaaa aatgacgaat
     1621 ttttaagcac aatcggatta attgcaaata taccgtcgat ttgccctctt tttttttaat
     1681 tgaaaaataa ctcttataaa ataacaggta cttcatatat aaagtacaaa attgaggata
     1741 caatggacat ttacaagatt aatggcggta aaaaaatatc gggtgacatt cccatttcag
     1801 gagcaaaaaa tgcggcttta ccaattattg taggaagcct tctcgccgat ggaacaacga
     1861 aacttactaa tgttcccgat ttaatggaca taagaacaac gataaaactg atagaacacc
     1921 ttggagcaaa gacatcattc gacaaagata ccaataccct tacaatagac gcgaaagaga
     1981 taatcaacgg tgaagttcca tacgatctgg taaaaacaat gcgcgcttcc atttatgtaa
     2041 tgggaccact tcttgccaga tgccaagagg ccgatgtctc tttgccggga ggttgtgcta
     2101 tcggtgcacg cccggttgac atacaccttc agggtttcga aaagcttggt acagatatta
     2161 atcttgatca cggttatgta aaggcacgca caaaaggcct taagggcgcg ctttatgtga
     2221 tgcccaaggt gtcagtaggg gcaacaataa acctgattat gggcgcagta cttgccgatg
     2281 gcgaaactgt actcgaaaac tgcgccatgg agccggatgt tgtagatctt ggaaattttc
     2341 taataaaaat gggtgccgat attcagggtc tgggcaccga aagaatcgcc gtaaagggtg
     2401 tctcttcgct gaaggctgtg gaatatcata ttatgcccga ccgaatcgag gcaggctctt
     2461 atcttcttgc cggtgccgcg gtgcgaggca atatcaaggt tacagatatt attcctgatc
     2521 atatcgaggc gctacttgac tcgcttaaag agatgggatt ccagataaaa accggctcga
     2581 actgggcgtc ggttgctcca tctgacaacc taaggggaat acttgtcgaa actactccgc
     2641 atcccggttt tccaaccgac cttcaagccc ccatgatggc tcttatgaca actgttcccg
     2701 gtatttcggt aatgatagag catattttcg aaaatagatt tacccacgtt gcggaacttc
     2761 accgtatggg cgcccagatc acaatagagg gaaaatgcgc catcattaaa ggtggtacat
     2821 cacttatgtc tgctcctgta atgatgtctg accttagagc cggagcagct cttgtaattg
     2881 ccgcgctggc cgctgaggga gagactgaaa ttcgaagaat ttaccactgt gaccgcggtt
     2941 acgaaaaatt atgtcacaag ttatcagccg taaatgccga tattaggagg gtaacaggag
     3001 gcatgtaaat gatcagagga atttataccg gcgcaagcgg aatgatatcg caacaggcaa
     3061 agatggacgt aactgccaac aaccttgcaa atgtcgacaa gacggctttt aagaaagata
     3121 caacaatctt caaggctttt cccgacatga taatccgccg aatcaatgat gacggcgtcg
     3181 gcattgtacc taccggatca tatgactcca tgccttatgt aggtaaactt ggtaccggtg
     3241 tagaggtgaa cgaggtcttt acccagtttg agcagggatc tcttcagcga actgaaaacc
     3301 agtttgacct ggcccttgaa ggtgaaggat tttttactgt tatgacggaa aagggtgaac
     3361 gccatatccg taacggatca tttacaatta atgacgaagg tattctgatg acccataacg
     3421 gaaatcctgt tatgggtgaa aacggaatta taagaataca aaaacacaat tttgtggtac
     3481 gtgaaaacgg tgaaattgtt gttaaccgcg acctactcga agagccggtt gtaggtcaaa
     3541 acgaaaacga gtggcaagat caggttgtaa ttgataagct gaaaattgtc gacttcgaat
     3601 acaaacgtca tctaaaaaaa gagggaacct caatgtaccg cgaaaccgaa gagtcgggtc
     3661 cggcactacc tcctcttaac ctcaaaatac atcaaggttt tctggaaaaa tcaaacgtaa
     3721 atgtagtgcg cggaatggtc gatatgattg aagttcaacg cagctacgaa gcaaaccaga
     3781 agagtataca aactgccgat caaacattgg gaaaacttat cagcgaagtc ggcagataaa
     3841 gcatgacact tgcgatataa aatatggctg ccttaaaacc cggcaaccat aaaattctaa
     3901 cggttgtttt tacctgttaa gaaaaacttc cagcgcttcc ggaaacggct gaagcgcgcg
     3961 cttgaatatt ccaaggctgt atgcgataac gagcccgtag tttgtaatag gaactccggc
     4021 ttccttacac tgtagcattc tagaaagcat ctcgcggcgg ttccacatgc acgcaccgca
     4081 atgaataacc agtttaaact ctgataaatc ttccggaaaa tcacgtccct gcatatttga
     4141 aaaatccagt tcccccccca catactggtt tagccatcgg ggaatcttta cagtaccaat
     4201 gtcatcagta atagggtggt gcgaacaggc ctcggcaata agtacacgat caccgggctt
     4261 aagattttcg acagcaatag ccccttgtgt ctgtattgcc aaatcccctt tgaaccttga
     4321 atagagtatc gaaaagctgg ttaacaatat atctttcggt gtgtcggcat ctgctttcaa
     4381 aaaagcctgt gaatcggtaa caaccagttt tggcggtgtt ttaagattat caattgcagc
     4441 tttaagctct ctctctttgg ctacaacgca gatggaatca ctatcaagca aatcacgtat
     4501 ggtctgtacc tgcggcaaga taagcctgcc ttttggcgcc tctttgtcta tgggaacaac
     4561 gagtaccacg gtctctcccg ggttaataag atcaccaatt atttgaggat tatccagaaa
     4621 ctcggggggc gcgttatcaa gcagagcttg cctgaaatct ttaattccgc tattcttcaa
     4681 ggccgaagta tgtacaaacg gaatttcttc ctcggcaagt ttttcaatca aagcattgtc
     4741 cggtttaacc tcgtcgcttt tgttaaaaac aacaataaat ttaatggcat actttgcgca
     4801 ctcctgacag attctttctt caaaatcgcc ccaggttccg tcagttacaa taactgctaa
     4861 atctgttctt ttgaaaacca gcgtggtttt ttttacccgt aaatcaccaa gctcaccaat
     4921 atcgtcaatt cccgcggtat caataaacaa caccggacct aacggcaata gttccattgg
     4981 tttttctaca gggtcggtcg tggttccggc aaaatctgat actatcgaga cctgctgccg
     5041 aaccatcaga ttaagaaggc tactcttgcc tacgtttcgt cttccgaaaa ttccaatatg
     5101 taatctgtta cccttcggtg tgttttgcat tactcaaacc ttcaaatatt ttcaattttg
     5161 ctgccaagcc tacatacagc ctcaaaagat actaacttgt caaattcacc actgctcaaa
     5221 taaccttcgc cgaccgacac atctcttatt gacttaccgc tttcggccga tttttgggct
     5281 attttgctac aagtgtcgta gccaagtgct gacacaagag ccgtaaccac ggcggtcgag
     5341 ttttcgacct ggccttgaca tacctgctca tttgcactta tgcccgctac acacaacgcc
     5401 aatattttgc aagattctgt gagcagctcc gcagaatcta acatgttata agccaaaaga
     5461 ggcataaacg cgttcagttc aaggtttccc gctgcacaaa gatgtgttac agtgctatcg
     5521 ttagacataa cctgatatgc cgcttgggac acgctttccg caataacggg attatatttc
     5581 cccggcataa tggaagaccc tgtctgaaca ggaacaagat ttatttcacc taacgggcct
     5641 gacaaaagcc gcaaatcacc agctatcttt aaaaggttaa cagcacaagc ctttaaaatt
     5701 ccacttacct cggcaaaaat atccgtgtta ctggttgcct cctgaagatt ctccgctcgc
     5761 gcgaaaccta ttcctgtcag gtcgcgcaat ttggtaacaa caccaaaaat atactcccgt
     5821 ggagcaccca aaccagtacc aatcgcggtg cctccaagat ttaccactcg aagccgctct
     5881 tcacatttat aaagcctcca gcgatcacgg ccaaaagcct cagcatatgt acccatttct
     5941 cgccccagtg tagtaagcac ggcttctcgc agttctgttc tccccagttt aacaatcttc
     6001 gcgaactcct tttctttatt ctcgaaagat tgctgcaaac ctgaaaccga actttcaaga
     6061 ctgtgcaaac tgttaatcag tgccaaccga agcgcagtag gaaaagaatc attagtcgac
     6121 tgaaagcagt ttacgtcatc tgtcggagaa acagtaaaat agtcacctgc acatttaccc
     6181 attagtataa gcgcgcgatt tgcaattacc tcgttcacat tcatatttaa agaagtgcca
     6241 gccccacctt gcatcatatc ggtaacaatc catgatgaga gttcaccttg cgacatctcg
     6301 cgagcggcat taataagcga ttctgcttta aaattatccc acacatcaag atctcggcac
     6361 attaaaagag ccgcgtgttt aacagtacca taggcccgaa ttagcgattc aggtgcaggg
     6421 cgcgacttga atggaaaatt attaagtgcc cgcatcgtgt ttatgccaaa cagtgaatcc
     6481 gccaatattt caagcgagcc taaagaatct gattccgtcc ggtaattatt gtccatcttt
     6541 tacacaggcc ttaattttta tttaaacaac tactatcatc attagcaaaa gacgtcacgt
     6601 tttccactct tgactttatc aaccatcttt atgctcaact ctcttggttg ttcatccatc
     6661 tctgaaataa ccttttctat taatgcttca cctctgtctt tagtctctgg tgatgcgtaa
     6721 tcatcaagat actcctgaaa tgtcgaaact gcattaggat cacaatgatg ccgtatcagc
     6781 cccggtttag ccagatccat aaagtcacca cctgttcgcc ctaaacggta acagcccgta
     6841 caaaaggagg ggacatagcc aagacgggca acatcggcaa taacctcatc aagcgttcta
     6901 tgatcaccaa gtgaaaactg agcctcgtcc gctacatcag ccccttcggc atatccaccc
     6961 ggattggttc gacttcctgc cgaaatctgt gaaacaccaa gcgcaaaagt atcttctcgc
     7021 atcttggggg tttcccttgt tgacattatt atccctgtat aaggaacagc acagcgtaaa
     7081 atcgcaacta tctttttaaa tgttaaatca tctacaggaa caggcggatg ctcagatatt
     7141 tctgatccgg ttgccggttc aagacgcggt acacttatgg tatgcggccc aaccccaaaa
     7201 tttttgtcca gataattaat atgttgcata agtgcaagaa tctcaaaacg ccaatctgcc
     7261 aacccaaaca ggactcctat gccaacatca tctataccag cctccatggc acgatccatg
     7321 gctgttaatc gccagtcata atcgcacttt ttacctgctg tatgaacttt gctgtaaaca
     7381 tctctgtcat aggtctcttg aaaaagctga taggtaccaa tagccgattt tttaagctgc
     7441 ctaaactgat caagatccaa aggagcaata ttcacattaa cacgtcttat ctcgtcgtga
     7501 ccattttttg tcttataaat ggtctctatc gaatcaagaa cgtattggaa accctcttta
     7561 ggatacgatt caccagccac aagcaaaacg cgtttatggc cctgtttttc taagattctt
     7621 atctcatttg ctatttcggt ctggctaaga gctcttcggt gaactgactt gtttcctgcc
     7681 ctaaaagcac aatacaggca ttcgtttgca caaagatttg aaatataaag aggggcaaat
     7741 attaccagcc ttcttccata aattgtattt tttataaact gagcggttgc gaagagctct
     7801 tcaagtcgct ctgaatcatt tattgaagtc aggcatgcaa cctcatctat ggaaagccct
     7861 tttaactctt ttgcttttaa taaaatatct tttaccctat ttttttcgca actcccgtta
     7921 tttaaaattt ctgtaatatt ctgagcgtta attatagaca taccagtctc cctatataat
     7981 cgttatatca aacagcttaa aacctatcat ttattacccg acattgtatt gactttgatc
     8041 aatttgtaaa aggaaaaatc tatattttaa atacttctct gacaaagttt tacctgtact
     8101 taataaatgc caccggtttc atggacacac attcaaaagt tgtgaaatta attgttgcaa
     8161 aaagagccta cttgaattat aaggattaga tagttattat ttaaatttaa tttacctata
     8221 caatttaata tattttttgg agtttataat gaatagtggt gaacaatcac agaaaacaga
     8281 agcgttagta ctggtcataa actgcggaag cagttctctt aaatatgaag tttacgatat
     8341 gccacaaatg ataagccgcg gaaaaggtaa agtcgagaga attggcatgg aaacgggaaa
     8401 tattgagcag aaatgtgaaa acggagtgta cacaaaagag actcccgttc ctgatcacaa
     8461 aaccgcattt aaatatgttg ttgaagcact aaccgacatc gaaaaaggca taataaaaaa
     8521 tatatctgat atcacagcaa tcggccaccg ggttgttcac ggtggagaaa gttattcaga
     8581 atcggtagta attgatgaat ccgtacttaa agctatcgaa gagtattcgg aactcgctcc
     8641 tctgcataat ccggccaatc ttacaggaat aattgaagca cagaatcagt ttaaaggaat
     8701 acataacgtt gccgtatttg atacagcctt tcatcagacc atgccacctg ccgcatatct
     8761 ttacggaata ccacgcgaac tgtacagaaa atataagatt cgccgttacg gatttcacgg
     8821 aacaagtcat cgttacgttg ctaacagagc tcttgatctg ctttacagat caagcgttaa
     8881 caccaacctt ataacctgtc accttggaaa cggtgcatct ataaccgcaa tagaacgcgg
     8941 acgttccgta gatacatcga tgggatttac ccctcttgaa ggcgttatga tgggtacacg
     9001 gtcaggcgac cttgatcctt ccattctgct ttttcttatg gataggggat tttcttcagc
     9061 cgagctaagc gaaatgctaa ataagaaaag cggcatgatg ggagtatcag aagtttcaag
     9121 cgatatgcgt gatcttaagg aagccgcccg caaaggcaat caacaggcca tcgaaacact
     9181 ggaagtctac acctacaaaa taaggaaata tatcggatcg tatgccgcga atctggtaaa
     9241 ggtggacgcc attgttttta caggtggtat tggcgaaaac aacgccgaca tacgtgagca
     9301 gatatgtcag cgcctagaaa acattggtat agttctggat aaggataaaa acaatagcat
     9361 acccaagggt ggcgagggaa tcatatcatc cgactactca caggccgtaa ttctgacaat
     9421 acccactaac gaagaacttc agatagctgt agacacctac aaacttgttt ttaatgaaga
     9481 gctttcttcg tttcaaagct aaggtctaaa aattataacc gctgataatt attgtattag
     9541 ttattggcgg ttacatatct gtatccggag acaaatccgg attaatacta tttttcattt
     9601 ttgagggcaa gagcctcata attccaaatg agataagcac agctcccgcc accatgttta
     9661 atagcttaaa aactattacc ggcaacacta ccttaacaaa ctaaataatt attacgctta
     9721 cccccataaa ggctatcgtt gccagaccgg ctgcagctcc aaaaatcaag agctcttttt
     9781 tattaagctt gtactcaatg gccttgttac taaaaagccc tgtccaaaaa actatagtta
     9841 aaggacttga tatcgttaaa agaaaaacag caacaaaact ctttatataa ctctctggtg
     9901 ttgcaacaga tgcctcgctc aaaacaaaat tgataccttt attaatcaac ataacaccaa
     9961 atacgattaa gacagccgat gatataaaaa gcatgatttt ttttatcgtt ggattttcta
    10021 gcagagcacc tacaccaatt atcgaaagca taatatacaa ataatcaaca actgcgacgc
    10081 cagctacggc agaca
//
LOCUS       DAOTGZ010000083         9919 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_3064
            NODE_8110_length_9919_cov_4.880170, whole genome shotgun sequence.
ACCESSION   DAOTGZ010000083 DAOTGZ010000000
VERSION     DAOTGZ010000083.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868088
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 9919)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9919)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:22:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,785
            CDSs (total)                      :: 2,751
            Genes (coding)                    :: 2,732
            CDSs (with protein)               :: 2,732
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 12 of 19
            Pseudo Genes (internal stop)      :: 5 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9919
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8110_length_9919_cov_4.880170"
                     /isolate="AMR_MDS_3064"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..2014)
                     /locus_tag="P5123_06995"
     CDS             complement(<1..2014)
                     /locus_tag="P5123_06995"
                     /inference="COORDINATES: protein motif:HMM:NF039580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-acyl-sn-glycerol-3-phosphate acyltransferase"
                     /protein_id="HRX16043.1"
                     /translation="MSDQKEKLLPGVLVDKLTGLRALFASFVCRKAELDDQSIRIIRD
                     YNEKGKLVYVSFQSSNIALLMLYRLLERNDIARPRLALECNPFLLQTPLYLIKKFFSK
                     IASLFKNKPNLLFSPDFVNNEFAEMRPILFSMLADTFFLKRYVEQKYDTLYYLIEMQK
                     KSDEPYYLMPQTIFWTRKPEKTPVTKDKTFRASSDKGFLSALLSTATPSYVHMMAPLN
                     LKELIEQHSEKTSEEIAHIIRDSLIEEQQKEDRVVLGPVLAPKNEMMERVLNHRNIRE
                     AIDRISKEDNRSRKKLRKKAYKYYREIAADFSITYIRFFGIVLNWIFRKIFSGFIIDD
                     DFIPVIKEAAKSGPVVLVPCHRSHMDYLILSYAFFQNRVIPPHIAAGVNLSFFPIGSV
                     FRHSGAFFIRRTFKNLKLYPEIFKQYLKTLLLDRYQLEFFIEGGRTRTGRLLYPRPGL
                     LSYLIEAADEGYAGDITFVPISINYDRVLEEGAYFREINGKDKKRESIGSILSGWKLL
                     KKDFGYVYVNAGETFTLSSVREGHTVSELVPKIGNRIVTEIAKAVTISPVAFVTLAML
                     LYRKKAVSHEDLTRSADYVYDVIRILNLGCSKVFYEKSITAIIEQVMESYSKDQIITK
                     MDDSDTGDHLYTLSDDNRRLIAFYKNSIAHHLLPLFTIANILYRSSD"
     gene            2205..5624
                     /gene="mfd"
                     /locus_tag="P5123_07000"
     CDS             2205..5624
                     /gene="mfd"
                     /locus_tag="P5123_07000"
                     /inference="COORDINATES: protein motif:HMM:TIGR00580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003690 - double-stranded DNA binding
                     [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription-repair coupling factor"
                     /protein_id="HRX16044.1"
                     /translation="MLLKPIIDSYISSREYNEFLERLDNQDSTRVLEGISRASFPLLC
                     SSCLITENKSIFAILPTPQLASDFAADCAAFIEEELIYRFPSPSVLPYEFLSPSEHIS
                     RERIRALYRLLSAKPALFIADTEAVLRLVPTPERLKRNILIFEKDEEYPFDDVIETLV
                     KYGYKREITVNSYGCFAVKGGIIDIFPPETDEPVRLDFFGDTLESIRYFDLESQVSRD
                     NIDHISIYPRNEFLLSSDERQTVEEYIKKSVLEGKKIPEHISEFVEGSELPDGAGIED
                     LLPHILGGSTIQDYINETVNLIIVDPPEVSAKRNSIENTYRDLYTRKSARELTVPVES
                     LISFEALTDLRQKAFIMQSFTSTPGSLRFGLKSIPTFHGKITALKEDLAERIQNGWKI
                     FISTAFEGQLRRLSDLLQEFKPATDEDKSDSSLVISLSSYSEGFEMEATKTLLLTDHD
                     IFGKSYRRKRSFKRRTSQPLKSFLDLAPGDYVVHINHGIGVFRTIERMSAGGFERDFL
                     MIEYADGDKLFVALDQLNMIQKYTGVDGKIPRIDSLGKKSAWNRIKKRVQESVEELAG
                     ELIEVYARRKALKGYQYPPDTLWQEEFEARFEYEETPDQLNAIEDVKDDMESSQPMDR
                     LICGDVGFGKTEVAIRAAFKAAMAGKQTAILVPTTILAMQHYKNFLKRFSDYPVAIEL
                     ISRFRSRKQINDTKSRMAGGDVDIVIGTHALLAKDIVFKNLGLLIIDEEQRFGVKHKE
                     RLKELRTQVDVMTMSATPIPRTLHMSMAGIRDLSTIMTAPENRQSIETYVMEENPDIL
                     QLAIRNELERGGQIFFIHNRVQTIDTHAALLSELVPEASIAVAHGQMNEHELEDILID
                     FLNKKYNILVATTILESGIDMPDVNTIIINRADTFGLSQLYQLKGRVGRSSLKGFAYL
                     FYPRHVPLNEVAQKRLRVISEYTDLGSGFKIAMKDMEIRGAGNLLGKQQSGNIMDVGF
                     DLYCQMLEDAVSRLKGERHSIYFRTPLFIKTDNYIPDEYISDQRQKIEFYKRFESCET
                     LEEIDRLTTEMKDRFGHYPEQVSILIQLETIRCIASNLYIEEILEGETSIRIRISEQT
                     KISTEKLLEVVKTDDRIMADPQNSDTLIFKTSEKETEKKLIALKKWLHDLC"
     gene            5963..7162
                     /locus_tag="P5123_07005"
     CDS             5963..7162
                     /locus_tag="P5123_07005"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRX16045.1"
                     /translation="MLKKFSQILLLIFVSTSFYSCGKPWVAKINGEKLSLEEFNNYYY
                     ANSIFVLKKDSREEIDALSTKPEEVQKYPYLSKQKFLEQIIRQRLVSEAIDEEGYLKD
                     DIDYDAFVYFQHLTIVVNRFSELKFEGKIDVTMEDIDKEFQSNRALYEQAPLDQAERY
                     IRQKIQYEKLQALSEEVLSEIKGRHKIERDEELVKKAIEKDPAKRPTEGVLLTIDGEE
                     ALTVPFFNALYYVQHTSNYDLKNEEVDKLAQSEEYVKQNPMLDRKLFFEQLVQMILFY
                     DEALNGEEFKMKEDKHLEYMLKIQEDIMKVGYYIRKKYRDELNPTDDEINKAYNQNKE
                     RFEGVPLDQAAMYIKQQILSQRLQAKGNEIVQNAKERSIIEYNMKALLEENKDKEDKP
                     SESPKED"
     gene            7349..8155
                     /locus_tag="P5123_07010"
     CDS             7349..8155
                     /locus_tag="P5123_07010"
                     /inference="COORDINATES: protein motif:HMM:NF024929.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M15 family metallopeptidase"
                     /protein_id="HRX16046.1"
                     /translation="MKINSIKLSSVIALSVILLYCCSSDCSDESPKNSIIKQHPLIIA
                     YPETIRSVENNHVTFYNNTVLSYDDGKIKNFEQKLDNSSIKDMFSQVYTPGVLRIPNK
                     NEDPGRFRNHDFFNTMYGETIKQIEKNLVTIIWMPGISGKKLRVTKVNNVDKHLQNVS
                     DKLILLSDSFHKYVNNPAGTYVYRKIAGTDRLSAHSYGIAIDINLDYSHYWRWDKHKD
                     PDNIIFKNRIPEEIVKIFESEGFIWGGRWYHYDTMHFEYRPELLTDKTNL"
     gene            8177..9409
                     /locus_tag="P5123_07015"
     CDS             8177..9409
                     /locus_tag="P5123_07015"
                     /inference="COORDINATES: protein motif:HMM:NF018743.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1343 domain-containing protein"
                     /protein_id="HRX16047.1"
                     /translation="MLKKISVYVGIISLTLFACGLPVKSSDKSLNTSEDKAATVKTGL
                     DILSETNCKKLKTKKVALVTNMTAVDNRNIHIVDIMKTNDIKIVKIFTPEHGFSAKLD
                     TKIENDKLGAIPLLSLYGKRRKLDPKELSDVDIVVFDIQTVGARYYTYKATLVYIMQA
                     AANAKKKLIVLDRPNPTGGVIVSGFMPQKGLTDKFTSIFPFPIRPGMTIGELALMFNK
                     EHNINCNLEVIAMQNWKRSMLFSDTNLPWHNPSPNINREEAAILYNGLGWLETTNISM
                     ARGTDLSFEMMGAPYIDSSKLIKKIGKVSGAVLKPVSFIPKAEYHKHYNKRCFGIQIS
                     MTDKRNFDPFPLGLLIYKALNELYPKDYISFSGLKTSSGRHNLKSDIQTRSMKDIIIS
                     TKKEIREFMKVRKKYLLY"
     gene            9476..>9919
                     /locus_tag="P5123_07020"
     CDS             9476..>9919
                     /locus_tag="P5123_07020"
                     /inference="COORDINATES: protein motif:HMM:NF014656.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endolytic transglycosylase MltG"
                     /protein_id="HRX16048.1"
                     /translation="MKIKNIITASIFTFVLACIVIVLTFFFLNLAPGDIKSQTEYSVQ
                     SGSTVRSNAQELKEYGLIKNTDFFVLLSYITGKKNLLTGTYTIFPGSSSLEILDKLSK
                     GSVVTVQVTIPEGYNIYSIASRFETAKICREDKFLFYAFDRTFLES"
BASE COUNT         3294 a         1901 c         1786 g         2938 t
ORIGIN      
        1 tgtcgcttga acgataaaga atattggcta ttgtaaaaag tggtaacaga tgatgtgcta
       61 tactgttttt ataaaaagca attaggcgac gattatcatc agacaaggta tacagatgat
      121 cacccgtatc cgaatcatcc atttttgtta ttatctgatc cttcgaatat gattccatca
      181 cctgttcaat aatagctgtt atactttttt cgtaaaacac ctttgagcaa ccaagattaa
      241 gtattcttat tacatcgtag acatagtcag cacttcgagt aagatcctca tgagatactg
      301 ctttcttgcg atagagcagc atagccaatg taacaaaggc taccggtgaa attgtaacag
      361 cttttgcaat ttcggtaaca atacggttac ctatttttgg tacaagctct gaaacggtat
      421 gtccttcacg taccgaactt aaagtaaatg tttcacctgc atttacatac acataaccga
      481 aatctttctt aagtaatttc caacccgaca aaatagatcc gatgctctct ctttttttat
      541 ctttaccgtt tatctctcta aaatatgccc cctcttcaag aacccggtca taattaatag
      601 aaataggcac aaatgttata tccccagcat acccttcgtc tgcagcttct attaaataac
      661 tcaataatcc aggccttgga taaagaagcc tacctgttcg tgttcgacca ccttctataa
      721 aaaattccaa ctgataacga tcaagaagaa gagtctttaa atactgcttg aatatctctg
      781 gataaagctt aagattttta aatgtacgcc tgatgaaaaa cgcccctgaa tgacggaaaa
      841 ctgaaccgat aggaaaaaaa gacagattaa caccagcagc tatatgaggc ggaataaccc
      901 gattctgaaa aaaagcatat gagagtataa gataatccat gtgactgcga tgacagggaa
      961 caagtacaac aggccccgat tttgccgcct cctttatcac ggggataaaa tcatcatcta
     1021 taataaaacc tgagaaaatt ttcctgaata tccagttaag aacaataccg aaaaatctta
     1081 tatatgtaat cgaaaaatct gccgcaattt ctctataata tttataagcc ttttttcgaa
     1141 gttttttacg agatctgtta tcctcttttg aaattcggtc aatagcttca cggatatttc
     1201 ggtgattaag aacacgctcc atcatttcgt tttttggcgc aagaacaggc cctaaaacaa
     1261 cacggtcttc tttctgctgc tcttcaatca gactatctct tatgatatgt gctatctctt
     1321 ctgaagtttt ttcactatgc tgttcgatca attctttcaa gttcaaagga gccatcatgt
     1381 gaacatacga aggggtcgca gtcgataata gagcggataa gaagcccttg tccgatgacg
     1441 ccctaaatgt cttatccttt gttaccggtg ttttttcagg ttttcgggtc caaaatattg
     1501 tttgaggcat cagataatag ggctcatcac tctttttttg catttctatc aaataataga
     1561 gtgtatcata cttctgctca acataacgct ttaaaaagaa ggtatccgca agcatggaga
     1621 aaagtattgg tcgcatctcg gcaaattcat tattaacaaa atccgggcta aaaagcagat
     1681 tcggtttgtt tttaaataag ctcgctattt tagaaaaaaa cttttttatc aggtatagag
     1741 gggtctgaag taaaaagggg ttacactcta acgcaagccg ggggcgagct atatcattgc
     1801 gctcaagcag tcgataaagc atcagcaacg cgatattgga actctggaac gatacataaa
     1861 ccagcttgcc cttttcattg taatcccgga tgattctaat agactgatca tcaagctcgg
     1921 cctttctgca gacaaaagaa gcgaacagag ctcttaaacc cgttagttta tcgacaagca
     1981 ctccgggaag caacttttct ttttgatcac tcatacaaac ctctccgatt ccacgtcttc
     2041 acaggattct tctgctaacc agctctattg tcaactattt gtcagcaaaa aattagtaac
     2101 caatgcccct gttttatgct tgataaacta ctttatatca aaaaattatt tacacacagg
     2161 taaactctcc atcctttgac attactacag ccaaggtgac atatatgctt cttaaaccca
     2221 ttattgactc ttatatttca agccgtgaat ataacgaatt tcttgagcgc ttagataatc
     2281 aggactcaac tcgcgttttg gaaggaattt ccagagcttc attccctctt ttgtgttcat
     2341 cttgcctaat tacagaaaac aaaagcatat ttgctatttt acccacaccg cagttggcct
     2401 ccgattttgc agcagactgt gcagcattta tcgaagaaga gcttatttac cgttttcctt
     2461 ctccatcggt tttgccttat gaatttcttt ctccatcaga acatattagt cgcgagcgta
     2521 ttcgagctct ttaccgtctg ctttctgcaa aacctgctct ttttattgca gacacagaag
     2581 ctgttttacg actggttcct actccagaga ggttaaaacg caatatactc atttttgaaa
     2641 aagacgaaga gtaccctttt gacgacgtta ttgagaccct cgttaagtac gggtataaac
     2701 gggaaataac ggtaaattcg tacggctgct ttgccgtaaa gggcggtatt attgacattt
     2761 tcccacccga gacagatgaa cctgtcaggt tggatttttt tggagatacg ctcgaatcaa
     2821 taagatattt tgatttggag tcacaggttt ctcgtgacaa tattgaccat atcagcatct
     2881 atccgagaaa tgaatttcta ttgtcttcag atgaaagaca aaccgttgaa gaatatatta
     2941 agaaatctgt tctcgaaggt aaaaaaattc cggagcatat tagtgaattt gttgagggat
     3001 cggaactgcc cgatggagcc ggcatagaag accttcttcc gcatatttta ggcggttcta
     3061 caattcaaga ctatattaat gagacagtaa atctcattat cgttgaccct cctgaagttt
     3121 ctgcaaaacg taatagtatt gaaaatacct acagagatct ttatacaaga aaatccgctc
     3181 gagaattaac cgtaccagta gagtctttaa tttcttttga ggcacttact gatcttcgtc
     3241 aaaaagcatt tattatgcag agctttactt cgactcccgg atctttacga tttggactca
     3301 agagcatccc tacttttcac ggaaagatta cagcattaaa ggaagatctc gcagaacgga
     3361 tacaaaatgg ttggaaaatt tttatttcaa cagcttttga aggtcaatta cgtcggctaa
     3421 gcgacctgct acaagaattt aaaccggcaa cggatgaaga caaatcggac tcttctttag
     3481 ttatttctct ctcgtcttat tcggaagggt ttgaaatgga ggcaacaaaa actttgttgc
     3541 ttactgatca tgacatattc ggtaaatcat acaggcgaaa aagatcattt aaacgaagaa
     3601 cctctcagcc tctaaaatct tttctagatc ttgctccggg agattatgta gtacatataa
     3661 atcacggtat cggagttttt cgaacaatcg aaaggatgag tgcaggaggg tttgaaaggg
     3721 attttttaat gatcgaatac gctgacggtg acaaactttt tgtagcgctc gaccagctca
     3781 atatgattca gaaatatacc ggtgtagacg gcaagattcc acgtattgat tcattaggca
     3841 aaaaatcggc ctggaataga ataaagaaac gcgttcagga gtcggtagag gagcttgccg
     3901 gagaacttat agaagtttat gccagacgca aagctcttaa aggctaccaa taccctcccg
     3961 acacactttg gcaggaagag tttgaagcac gtttcgaata cgaagagaca ccggatcagc
     4021 tgaacgctat agaagatgtt aaagacgata tggaatcatc acaacctatg gacagactaa
     4081 tatgtggtga tgtcggcttt ggtaaaaccg aggtcgccat tcgggctgca tttaaggcag
     4141 ccatggcggg taaacagaca gcgattctgg ttccaactac catactcgca atgcaacact
     4201 ataaaaattt tttaaaacgt ttttctgatt atccagttgc cattgaactt atttcacgtt
     4261 tcagatcgcg aaaacagatt aatgacacaa agagccgtat ggcaggcgga gatgttgata
     4321 ttgtaattgg aacccatgcc cttttggcaa aggacatagt atttaagaat ctggggcttc
     4381 tgattattga tgaggaacag cgatttggag ttaaacataa agagaggtta aaagagcttc
     4441 gaacccaggt tgatgttatg acaatgtcgg caactccgat accacgaaca ttacacatgt
     4501 ccatggcagg cattagagat ctatcgacaa tcatgaccgc tcctgagaac cggcagtcca
     4561 ttgaaacata cgtaatggaa gagaacccgg atattctgca acttgcaata agaaacgagc
     4621 tggaacgagg tggacagatt ttctttattc ataatcgtgt tcagacaatt gatactcacg
     4681 cagccttgct ttctgaactt gtgcccgaag catcgatagc cgttgcccac gggcagatga
     4741 acgaacatga acttgaagac atacttatag actttttaaa taaaaaatat aatatactag
     4801 tagcaaccac aattctggag agcggtattg acatgccaga cgttaacaca atcattataa
     4861 atcgtgcgga cacgtttggg ctttcacaac tttatcaact aaaaggtcgt gttggcagat
     4921 cctctcttaa aggttttgcc tatctgttct atcctcgcca cgtgccttta aacgaagttg
     4981 cacaaaaacg tctcagagta atatccgaat acaccgatct tggtagcggc tttaaaatag
     5041 ccatgaaaga catggagatt cgtggcgcgg gtaatctatt aggcaagcaa caatccggaa
     5101 atattatgga tgtcggtttt gatctttact gtcaaatgct cgaagatgcc gtaagcaggc
     5161 tcaaaggcga aagacattct atctattttc gaactcccct gttcattaaa acagataatt
     5221 atattccaga tgagtatata agcgaccaaa gacaaaaaat tgaattttac aaacgttttg
     5281 aatcatgtga aacccttgaa gagattgatc gtttgactac agaaatgaaa gatcgattcg
     5341 gacattatcc agaacaggtc tcaattctta tacagcttga aacaatcaga tgtattgcct
     5401 ctaatttgta cattgaagag attcttgaag gagaaacttc tattcgtata agaatatcag
     5461 aacagacaaa aataagtacc gaaaagctgc ttgaggttgt taaaacagac gataggatta
     5521 tggctgatcc acaaaatagc gatactctga tatttaaaac tagtgaaaaa gaaaccgaaa
     5581 aaaagcttat tgctctaaaa aaatggttgc atgatctatg ctgacacaat aacaagatgc
     5641 agaaaacttc ttaattgttt tctattgtat gaatatcgct aaattattag atatcgcctg
     5701 taactttttt tgttacaata ttacacactt tgtatcatta aggaacttcg aaaaattaca
     5761 atgttccaga gggtaacatg gttttactgt aaataattat tgaacaatgc gactcgcata
     5821 cattactgca taaacaagtc tcatttattg ccatgtttaa aagttattct gataactaat
     5881 catttttctg atgcaaagcc gttactaagc tttcacgaag agttgtgtct cttctaatta
     5941 atattatatc aaggatcata aaatgttaaa aaaattttct cagatactcc ttttgatttt
     6001 tgtttccaca tcattttatt catgtggcaa accttgggtt gccaaaatca atggtgaaaa
     6061 attatctctt gaagagttta ataactacta ctacgccaac agtatatttg ttctaaaaaa
     6121 agattcccga gaagagatag atgctctctc aaccaaacct gaagaagttc aaaaatatcc
     6181 ttaccttagc aagcaaaaat ttcttgagca aataatcaga caaagacttg tttctgaagc
     6241 aattgatgaa gaaggctacc ttaaagacga tattgattac gatgcatttg tctattttca
     6301 gcatctgaca attgttgtta accgtttttc cgagctaaaa tttgaaggca aaattgatgt
     6361 tactatggaa gacatcgata aggaatttca atctaaccgt gcactttacg agcaggctcc
     6421 ccttgatcag gccgagcgct atatcagaca aaaaattcaa tatgaaaagc ttcaggcttt
     6481 atctgaagaa gtactatccg aaatcaaggg tcggcataaa attgaacgag acgaagagct
     6541 tgtaaaaaaa gctatagaga aagatcctgc taaacgtcca acagaaggag tccttctgac
     6601 tattgatggt gaagaagcat tgactgttcc attttttaac gccctctatt atgtacagca
     6661 tacatccaac tacgatctaa aaaatgagga ggttgataag cttgcacaat ctgaggagta
     6721 tgtaaaacag aatcccatgc tggatcgaaa attatttttc gagcaactgg tacagatgat
     6781 tctcttttat gacgaagctc ttaacggtga agagtttaaa atgaaagagg acaagcatct
     6841 agagtatatg cttaaaatac aggaagatat aatgaaagtc ggttattata tacgtaaaaa
     6901 atatcgtgac gagttgaacc caacagacga cgagataaac aaagcctaca accaaaacaa
     6961 agaacgtttt gaaggtgttc ctcttgacca agctgccatg tatataaaac agcagattct
     7021 tagccagcga ttacaggcta agggtaacga aattgttcaa aacgcaaaag aacgcagcat
     7081 aatcgaatat aatatgaaag ctttattaga agaaaacaaa gataaagaag ataagcctag
     7141 tgagtctcct aaagaggatt aatgccgtac aattttaaac tctttgttaa ttcttaaaga
     7201 ctatatccgg catgtgttat tgtgccggat attgttattt ttaatgttta attatctgag
     7261 tatatcttaa aatatgtcaa ctcgtacctt tgcaaatgca aacaagcttt taacttgacc
     7321 taaatcaata ccaaaataat ttaaatttat gaaaattaat tctataaaat tatcctcagt
     7381 tattgctctc tcggttattt tgctatactg ttgctcttca gactgttctg acgaatcacc
     7441 aaaaaacagt attataaagc aacatccgct aattattgct tatcccgaaa caattagatc
     7501 agtggagaat aatcatgtta ctttttataa taacactgtc ttatcttatg atgacggcaa
     7561 aataaaaaat tttgagcaaa aactcgataa cagcagtatc aaggacatgt ttagtcaagt
     7621 gtatactccc ggagtgttac gaattcccaa taaaaacgaa gatccaggac gatttagaaa
     7681 tcatgatttt tttaacacca tgtatggaga gacgattaaa caaattgaga aaaatctggt
     7741 tacaattata tggatgcccg gtatctctgg taaaaaactc agagttacaa aggttaacaa
     7801 tgtcgataaa cacttacaaa atgtttcaga taaattgata ctattatctg atagttttca
     7861 caaatatgtt aataaccctg caggcactta tgtttaccga aaaattgccg gaaccgaccg
     7921 acttagtgca cactcatacg gtattgccat agatattaat cttgattatt ctcattattg
     7981 gcgttgggat aaacacaagg atccagataa tattattttt aagaatcgaa tccccgaaga
     8041 gattgttaaa atatttgaat ctgaaggttt tatatggggg gggcgttggt atcattatga
     8101 taccatgcac tttgaatacc gtcccgaact tcttactgac aaaactaacc tataaacaac
     8161 agaggctaaa aactatatgt taaaaaaaat ttctgtttat gttggcatta tatcactgac
     8221 tttgtttgcc tgcggtcttc ccgttaagtc atcggacaag tctttgaata catcggaaga
     8281 caaggctgca acagtcaaga ctgggctgga tattttgtcc gaaacaaact gcaaaaaact
     8341 aaaaaccaaa aaagttgccc ttgtaacaaa tatgactgca gttgataacc gaaacataca
     8401 tatagttgat atcatgaaaa ccaacgatat caaaatagta aaaatcttca cacctgaaca
     8461 cggtttttcg gcaaagctgg acactaaaat agaaaacgat aaacttggtg caatacctct
     8521 tctttctctt tacggtaaac gccgcaaact tgatccgaaa gaactttctg acgtagacat
     8581 tgtagttttt gatatacaaa ctgttggtgc tcgttattat acgtacaaag ctactctcgt
     8641 atatataatg caagcagcag caaatgcaaa aaaaaagctt atcgtacttg accgccccaa
     8701 tccgaccgga ggtgttattg tctctggttt tatgcctcag aaaggtttaa ctgacaagtt
     8761 cacgtcaata tttcctttcc ccattcgccc aggaatgaca attggcgagc tcgctctaat
     8821 gtttaataaa gaacacaata ttaactgtaa tcttgaagtt atagcaatgc aaaactggaa
     8881 aagatccatg cttttttcag atacaaacct tccctggcac aacccttctc caaacataaa
     8941 tcgcgaagag gctgcaattt tatacaacgg actcggctgg cttgaaacca ccaatatatc
     9001 aatggcaaga gggacagatc tctcctttga aatgatgggc gctccatata ttgattcatc
     9061 taaattaata aaaaaaatag gcaaggtttc aggagctgtt cttaaacctg taagctttat
     9121 tccaaaagct gaataccaca agcactataa taaacgttgt ttcggaattc aaatctcaat
     9181 gacagataaa cgaaattttg atcccttccc tctcggatta ctaatatata aagccttaaa
     9241 tgagctttac cccaaagact atatctcctt ttccggcctc aaaacaagca gtggtcgcca
     9301 taatcttaaa tcagacatac aaaccagatc tatgaaagat ataatcataa gcaccaagaa
     9361 agaaattcgt gaattcatga aagttcgcaa aaaatatcta ttgtactaaa aatagccaga
     9421 ttgcgtaaat tttggaattc ttttagaatg ttatcaaatt aaggttttaa tagttatgaa
     9481 aattaaaaat attattacag cttcaatatt cacttttgtt ttagcttgta tagtcattgt
     9541 tcttaccttt ttctttttaa atttggcgcc tggggatatt aagtcacaaa ctgagtatag
     9601 cgtacaaagc ggctcgaccg ttagatctaa cgcgcaagag ttaaaagagt acggcttgat
     9661 aaaaaacacg gatttttttg tgcttctttc atatatcacc ggaaaaaaaa atttgttaac
     9721 aggaacctat actatttttc caggatcaag cagtctggag atacttgata agctctctaa
     9781 aggatctgtt gttaccgtgc aagtaacaat tcccgaaggg tataatatat attctattgc
     9841 tagccgtttt gaaactgcaa aaatatgccg tgaggacaaa tttctttttt acgcctttga
     9901 cagaactttt cttgaatct
//
LOCUS       DAOTGZ010000084         9911 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_3064
            NODE_8121_length_9911_cov_6.726664, whole genome shotgun sequence.
ACCESSION   DAOTGZ010000084 DAOTGZ010000000
VERSION     DAOTGZ010000084.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868088
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 9911)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9911)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:22:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,785
            CDSs (total)                      :: 2,751
            Genes (coding)                    :: 2,732
            CDSs (with protein)               :: 2,732
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 12 of 19
            Pseudo Genes (internal stop)      :: 5 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9911
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8121_length_9911_cov_6.726664"
                     /isolate="AMR_MDS_3064"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..382
                     /locus_tag="P5123_07025"
     CDS             <1..382
                     /locus_tag="P5123_07025"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRX16049.1"
                     /translation="KDDIIKEKKDKTVPVLIGVFVFLVVIINTKLGIDKHSSSYFNPI
                     TWAELFKCIPLLALLSIAASVATYLYRSYIMKHNFSDYSICIKCEKVYRNKKNVKAEN
                     MMICKCGGTVVNLNRVKWVDDENY"
     gene            complement(561..915)
                     /gene="ssrA"
                     /locus_tag="P5123_07030"
     tmRNA           complement(561..915)
                     /gene="ssrA"
                     /locus_tag="P5123_07030"
                     /product="transfer-messenger RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00023"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00023"
     gene            complement(1294..2154)
                     /locus_tag="P5123_07035"
     CDS             complement(1294..2154)
                     /locus_tag="P5123_07035"
                     /inference="COORDINATES: protein motif:HMM:NF038196.1"
                     /note="N-terminal region resembles flavodoxins. C-terminal
                     ferrodoxin region binds two 4Fe-4S clusters; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EFR1 family ferrodoxin"
                     /protein_id="HRX16050.1"
                     /translation="MNNIYYFTGTGNSLCVANRIAAADKGTKVSRVTKKSLLKPVLLK
                     GKVGIVFPVYAWGAPAMLEKFLKTAGYTGVEYLYIFATYAGSERNALHSCASVIRKSG
                     GTVNAAFSVKMGNNYIIASNTASPALAERMMQKREDQLAAMINSIIEKRDLPVKKSLS
                     PLAFLASGLVHPMFAKYYGKKVSQQFSVTDSCNSCGICVKVCPSNNVRLLDNPDKLNS
                     SSKAAGKAQKLDCNTTIVPDKKPVWGTECEVCLACINLCPQKAILYKNSHSERIRYCN
                     PEVKVSDLFV"
     gene            complement(2292..2705)
                     /locus_tag="P5123_07040"
     CDS             complement(2292..2705)
                     /locus_tag="P5123_07040"
                     /inference="COORDINATES: protein motif:HMM:NF016370.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF559 domain-containing protein"
                     /protein_id="HRX16051.1"
                     /translation="MQRKKDFIENAVMLYNGIKVNTPKYVIELARQNRQNRQNLTVSE
                     QTIWSQLRNKQLNDYKFRCQHPIYRYILDFYCHKAYLAIEIDGNIHKERVDYDSYRDK
                     FLEDIGITTLRFSVEEVMSKNSSVINKILTVLSTK"
     gene            2911..3174
                     /locus_tag="P5123_07045"
                     /pseudo
     CDS             2911..3174
                     /locus_tag="P5123_07045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016518798.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_process: GO:0008219 - cell death [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="Txe/YoeB family addiction module toxin"
     gene            complement(3240..4175)
                     /locus_tag="P5123_07050"
     CDS             complement(3240..4175)
                     /locus_tag="P5123_07050"
                     /inference="COORDINATES: protein motif:HMM:NF025221.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4197 family protein"
                     /protein_id="HRX16052.1"
                     /translation="MKKRVLTLFLVLLISGSGCDSSGSSSSTPADLAEGIRGALRWCS
                     EQATGTLGKANGFLLDKVCKILLPEPAQETLDYIKSSTAAKTALNAAIEIAFLGTDID
                     DIDSFEDAVIKSMNTAAEEAVADTETYQAFADVISSLSIDGTFDILRGTVPATSRAEG
                     EEPVTETPYAATIYLKEMTHDPLLTAFSDIIDPLFDQKLGRAAYRNRAGVSFSLNELW
                     GYYKTAVSTYNAGAGFLYDEVDVPADTIGTHVTQKALDGLFYKVGEFELKMRDDYATL
                     FDIASDIYEAFKWADENAPDGLTDLIESGLDYLLP"
     gene            4612..6108
                     /locus_tag="P5123_07055"
     CDS             4612..6108
                     /locus_tag="P5123_07055"
                     /inference="COORDINATES: protein motif:HMM:NF014147.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 30 beta sandwich
                     domain-containing protein"
                     /protein_id="HRX16053.1"
                     /translation="MNTNFKTRKRKAGYNLLLILALFLAAFFTQSCTSRKGGTVNQFS
                     TDKDGKEQKSKFSYRFKKHDSADLVIETGIPAQHIDGFGGAFNEAGWEALQILDQQSR
                     DTVIKQLFDDKEGAAFNLCRVPIGSSDYALKRYSHNETQNDYQMKNFSIERDHKYLLP
                     YIKAALKHQPNLAVWGSAWTPPTWMKDNKSFNSGKFLDDPKIYQAYALYLAKFIEAYR
                     AEGINLTEVAIQNEPEVLRPYPTCGWDGEQYYKFIKNHLGPLFKKKELNAGIMLGTFN
                     NADALRNYIRPILSDPECNAYISSVGFQWDGVDILYDTRQEFPGKRYVQTETECGNWS
                     WPGKWNEEIPDFVYDRQKAQNDYSYAVYTFNRVLDYFREGANSYMLWNMVLNEKGLNN
                     NEEDSWPQNAAIVVDTQTKKAVLTPMYHAFRHFSHYVNKGAHYLPSTSKENFAAAFLN
                     PDGSIVVVMKNQQSSEKEVNVQFGDKFFSATLPANSFTTFVAEPALEI"
     gene            6305..7483
                     /locus_tag="P5123_07060"
     CDS             6305..7483
                     /locus_tag="P5123_07060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013513002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HRX16054.1"
                     /translation="MPYNKSPECLDLENLLGITEELNHIKDIDSLLDHILHNARQFCK
                     AEAGSIFLREKDHLVFSYVQNDVLVKREKNYNKRIYDKFTIPINRESIAGYVADTGKP
                     LKIDDVYAIESSVPYSFNGSFDEMSNYKTVSMLTIPLITSRDNITGVIQIINPKSEND
                     VTVFTEIHCQYVTFLANNASVAIERAQMTREIILRMIRMAELRDPKETGAHVNRVGAY
                     SIEIYDRIAKKRGESPEIIRRTKDHLRIAAMLHDVGKVAISDTILKKPARLNEEEFKI
                     MQTHCASGADLFEGNVSDLDKLSTQIALTHHEKYDGSGYPNGLEGKLIPLPGKIVALA
                     DVYDALISKRVYKEEWPEEEVLNHIKSESGKHFDPEVVEAFFDVHDILKAVGKRYPDK
                     "
     gene            7639..8946
                     /locus_tag="P5123_07065"
     CDS             7639..8946
                     /locus_tag="P5123_07065"
                     /inference="COORDINATES: protein motif:HMM:NF012234.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HRX16055.1"
                     /translation="MKESIQPELIPFDRMREQVEKNLTTYISQSSECLELPKITYEKG
                     VSTISFPLPYFNETTIFTIISIIKAHIENVRIHSFDDGYYAFQMMNRNLFNTEGLLDN
                     LKVEIFGLSSGSRTEISKKGNLSQEEINFSIEIFKELYEVEQDNPETALRRLGASVYH
                     DHGSLDWNYIAGYEDVKRKIRESIIMPLQNPDIYDSIARLTRRSFETNRPSAILFDGP
                     PGVGKTTIARIIAGEVKIPLVYVPVESIMSKWYGQSSQNLSAIFDNTEFLGSAILFLD
                     EIDSLAGSRNSSMFEATRRILSVLLRRLDGIDSISNTITIGATNRRGDLDPALISRFD
                     QTISFPLPDAKERAAIFSGYARHIEEKDLLSFGEQTAGISGRSIKDICEYTERRWARK
                     IIIQKAEAVAPPAEYYRYSINSWMGDNFSSTTERGDEVVEPLG"
     gene            complement(8982..9872)
                     /locus_tag="P5123_07070"
     CDS             complement(8982..9872)
                     /locus_tag="P5123_07070"
                     /inference="COORDINATES: protein motif:HMM:TIGR01784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rpn family recombination-promoting
                     nuclease/putative transposase"
                     /protein_id="HRX16056.1"
                     /translation="MIVRKCNRKAKPHYNAYKYLFSNKRIFLQLLNSFVHEDFVKNLT
                     EEGLELADKSFVSQDMLDRESDLLYKININGKDAYIYILIEFQSTPDKAIPVRMFQYI
                     LLLYDLLLKNNKRGLLPNVFPLLLYNGSKKWNVPRNIRDLINKNMPDRFIPSYEYFAV
                     ILYEVSDKYLDKLSNLIAAVILMENAGDKNKLRIAIDRIVMYIRDEDPSDIRDYFIWL
                     KNMFSVELKDTKLDSIRNLMEVKPMLAELVEKIKNENWNEGKIEGKTETARAMLKDGF
                     SVANISKYTGLSEKEITAIK"
BASE COUNT         3161 a         2142 c         1851 g         2757 t
ORIGIN      
        1 caaagacgat attattaaag aaaaaaaaga caaaacagta ccggttttga taggggtttt
       61 tgtatttctg gttgtaatta tcaatacaaa acttggaatc gacaagcaca gttcatcata
      121 ttttaaccct ataacatggg cagaactttt taaatgcatt cctcttttag cattactttc
      181 aatagctgct tccgtagcga catatcttta caggtcatat attatgaaac ataatttttc
      241 cgattattcg atatgtatta aatgtgaaaa ggtgtacaga aacaagaaga acgtaaaagc
      301 tgaaaacatg atgatttgta aatgtggcgg aacagtggta aacctgaaca gagtaaaatg
      361 ggttgatgac gaaaattatt aagaaattat caataaatag gtgtttcaat ttcatccgca
      421 ggtacataaa atttcgcacg aacggctttt gagaaatcat attctttttt catactttcc
      481 tctaaaaata attacaaaaa aaccaccgcc cctgaccagg taagaaccag ccaaaaaccg
      541 tgattgtaat gttaagtaat ctgtggagac ggcggggatc gaacccgcgt cctgcaattc
      601 ataacagcaa cgtctacatg cttattctat ccattaaatt taagagacag gccggaggac
      661 aaaagaccct acctgaaacc gatcactcta taaattcgcc cggctcacga gtgaagatga
      721 gacagactat ccttcattga tgacgtccga taagccgccg aaggagggca acctataaga
      781 cgtagctaca ctattaggca gctagtgcta gattttcatc tgcaattaca tttaggcctg
      841 acatttttac gatgtgccag cgcaatcggc atgcaattac tgctacaaca gagcagtcga
      901 aaccaaggcg tccccttttc aaagatccgc agtaaatata agaaaaaacg tcaattcgtc
      961 aagcataaaa acctgtagtt gacaactctt atctctaaaa gagaaagctg acttatctga
     1021 aaaaatcaag gataaaccgg tcattcttta cttcgaatag ccagcaattc acaaacacgg
     1081 aaaattataa taatgtgaat cgggaaatct cttaaccgaa actcctccgg cttttcataa
     1141 taggcaacct ctagaaatta acttttcaac aaggttgcat atgcgagcag caagagtcat
     1201 cttgcgacca gcataggaac ttgcgaaaaa ttcctttaga ataggcgctt aagccatgtt
     1261 gccctctgga acatggtaat ttttcgaagt tccctataca aacagatcag aaaccttgac
     1321 ctccggattg cagtaacgta ttctttcaga atgtgaattt ttgtacaaaa ttgccttctg
     1381 cgggcaaaga ttaatacagg ccaagcacac ctcgcattcg gttccccata ccggtttttt
     1441 atccggcact attgttgtat tgcagtcaag cttctgcgct ttgccggctg ctttcgaaga
     1501 cgaattcagt ttgtcaggat tatccaacaa cctgacatta tttgacggac aaaccttcac
     1561 acatattcca cagctgttac agctgtctgt aaccgaaaac tgttgtgaaa cctttttgcc
     1621 gtaatatttt gcaaacattg ggtgaaccaa cccgcttgct aaaaaggcta acggagagag
     1681 tgatttttta acaggcaaat cccgcttttc aataatcgaa ttaatcatcg ccgccaactg
     1741 atcctcccgc ttttgcatca tcctttctgc caaagccggt gacgcggtat ttgaagcaat
     1801 tatatagttg ttgcccatct ttacagaaaa ggccgcatta acagtgcctc cggattttct
     1861 gataacagaa gcacagctat gtagggcgtt gcgttcactt ccggcatagg tcgcaaaaat
     1921 atacagatat tcgacaccgg tatagccggc tgttttcaga aacttttcca gcatggccgg
     1981 agccccccag gcatacaccg gaaaaacaat tcctaccttg ccttttaaaa gaaccggttt
     2041 taaaagagat ttttttgtaa cacgtgaaac ctttgtgcct ttatccgcgg cggcaattct
     2101 gttggcgaca cagagagaat ttccggtgcc tgtaaaatag tatatgttat tcatagggtc
     2161 tcctcttaaa aatatatctt cacaaaaatg gatgacggta gtatacaacc gtcctgtaaa
     2221 atgcgcaaaa gctgacttct acccgtaaat cttaatctat gcgcccccaa cccccaaggg
     2281 gggcttttta ttcattttgt tgataataca gttagtattt tattaattac cgaactattt
     2341 ttactcatca cttcctcaac tgaaaaacga agagttgtaa ttccaatatc ttctaaaaat
     2401 ttatcccgat aactgtcgta atcaacccgt tctttatgaa tatttccatc aatttcgatt
     2461 gctaaatatg ctttgtgaca ataaaaatct aatatgtatc ggtaaatcgg atgctggcaa
     2521 cggaacttgt aatcattaag ctgtttgttc ctcaattgtg accatattgt ttgttcagaa
     2581 acagtcaggt tttgtctgtt ttgtctgttt tgtctggcta attcaataac atatttgggt
     2641 gtatttactt ttattccatt atataacata acggcatttt ctataaaatc ttttttcctt
     2701 tgcaataact atttaaagtc cccctttggg ggatttaggg ggctcaagat ccaacataag
     2761 taaattaata aatctaagca gaatttagtc aataaaagag agaggctacg acatatcaca
     2821 acttgaaaag gtgcctcaaa aatgaggcac cggaatcgaa agatttctgc ttaacctgta
     2881 aagccgttct taaaaaagta gttttcgaag atctattgac tttttacaga tgaagcgtgg
     2941 aaaaattatc tttactggtc agaaaccggc aaaaagatac taggaaaaat caatgacctg
     3001 atcaaagaaa tagaccgtac accttttgaa ggcaaaggaa aatctgaacc attgaaatat
     3061 acataaaaag acttttggtc cagaaaaatt agtcacaaac accgaattgt atatatgata
     3121 aaaactgatc agatctattt tatctcattc aggtatcacc atcgcgctga ataatcgcct
     3181 atatgcgaaa acaactattc agcattttaa caaatctgac aacaactaca agatccgaat
     3241 catggcagca gataatccag accactctct ataagatcag tcaatccgtc tggtgcgttc
     3301 tcatccgccc atttgaaggc ttcgtaaata tcactcgcaa tatcgaacag agtcgcgtag
     3361 tcatctcgca ttttgagttc aaattcacca accttgtaaa agagcccgtc cagtgccttc
     3421 tgggtaacat gtgtacctat tgtatcagcc ggaacatcaa cctcgtcata aagaaaaccg
     3481 gcacccgcat tatatgtaga tacagctgtt ttataatacc cccaaagctc attaaggctg
     3541 aacgaaacac ctgcacggtt gcggtaggca gctctgccaa gtttctggtc aaacagcggg
     3601 tctatgatat ccgaaaaagc cgttagcaga ggatcatgag tcatctcttt aagatagatc
     3661 gttgcggcat agggagtctc tgtcaccggt tcttcaccct ccgcccgtga tgtcgcgggt
     3721 actgttcccc gtaaaatatc aaaggtgccg tctatcgaaa gcgacgatat aacatctgcg
     3781 aatgcctgat aggtttcggt atcggccacg gcctcttcgg cagcagtatt catggacttt
     3841 attaccgcgt cttcaaacga atctatatcg tcaatatcag ttcccaggaa ggcaatctca
     3901 atagcagcat ttaaagcggt tttggcagca gtactggact ttatataatc aagagtctcc
     3961 tgtgcgggct ctggcaaaag tattttgcaa accttgtcca gaaggaaacc gtttgcctta
     4021 cccagggttc ccgtagcctg ctcggaacac cagcgaagag cgcctcttat tccctctgcc
     4081 aaatccgcgg gagtgcttga cgagctgccc gaggaatcac atcctgatcc cgatattaac
     4141 agcaccaaaa acagggttaa aactctcttt ttcataatct tctccgatag taacagataa
     4201 attctgatca taattgctac tgtatttttt aatagaaata ccctaataat atataaagat
     4261 catttttttt caaccaaata tccataaata gaacaacctt ccattcgtat cagttcagaa
     4321 ctgtttattc agacatacat aaatactctt agacaaaaca gaatatgaat tcaggatttt
     4381 tatgattctt aaaagctttc tttaataccc taagccagtt ccggaaattg ccgaaatgcc
     4441 gcaagataaa atttcaatca aatccgcgat accatacatt caacctaaga cttaagtgcc
     4501 aaacattgtt ttgaccaaaa gtcttaaaca accggtttcg ggatcgcttt acataataga
     4561 taaccatgca ataatttagc tatcagaaac aattttttac aggagtatac tatgaacact
     4621 aatttcaaaa ccagaaaaag aaaagccggt tacaatttat tattaatatt agcccttttt
     4681 ctggcagcat tttttacaca atcatgtacg tctcgaaagg gtggcacagt caaccaattt
     4741 agcaccgata aagacggcaa ggagcaaaaa agtaaattta gctaccgatt caaaaaacac
     4801 gactctgccg atcttgttat tgagaccggt attccagcac aacatattga cgggtttgga
     4861 ggagccttta acgaagcagg atgggaagcc cttcaaatat tggaccaaca aagccgcgat
     4921 acagtaataa aacagctttt tgatgataaa gagggggcag cctttaatct ttgtcgtgta
     4981 cccatagggt caagcgacta cgcactaaag cgttacagcc acaacgaaac acagaacgat
     5041 taccagatga agaatttttc tatagaacgc gaccataaat atctgttgcc ctatatcaag
     5101 gcagccttaa aacatcagcc aaatcttgct gtatggggat cggcctggac tcctccaacc
     5161 tggatgaaag ataacaaaag ttttaacagt ggaaaatttc tggatgaccc caaaatatat
     5221 caggcttacg ccctgtacct agcaaaattt atcgaagcat atcgcgccga aggcattaac
     5281 ctgaccgaag ttgcgataca aaacgaaccc gaggtattgc gcccataccc gacttgcgga
     5341 tgggatggtg aacagtacta caagttcatt aaaaatcacc ttggacctct tttcaagaaa
     5401 aaggagctta acgcgggcat tatgcttggg acatttaaca acgccgatgc tcttcgaaac
     5461 tatatacgtc ccattctcag cgatccggaa tgcaacgcat atatcagctc ggtaggattt
     5521 caatgggacg gagtggatat tctttatgac acacggcagg aatttcctgg caaacgctat
     5581 gtacaaaccg aaaccgagtg cggaaactgg agctggcccg gcaaatggaa cgaagagatc
     5641 cccgattttg tttacgacag gcaaaaagca caaaacgatt atagctatgc agtttacaca
     5701 tttaaccgtg tgcttgacta ttttcgcgaa ggcgcaaatt cgtacatgct ttggaacatg
     5761 gtactaaacg aaaaaggtct aaacaataac gaagaggatt catggccgca aaatgccgca
     5821 attgttgtag atactcaaac caaaaaggcc gtgcttacac ccatgtatca tgctttccgt
     5881 cattttagcc actatgtcaa taaaggcgca cactatcttc catcaacatc aaaagaaaat
     5941 tttgccgcag cctttcttaa ccctgacgga tcaatagttg ttgtaatgaa aaaccagcag
     6001 agcagcgaaa aagaggttaa tgttcaattc ggggacaaat ttttctccgc gacacttcct
     6061 gctaactctt ttacaacctt tgtggcagaa ccggctttag aaatatagcg acctcctgac
     6121 aacggttata cttctgcaaa agggtagaag tataaccgtc tgttttgctc tataaacagc
     6181 aattaatcac ctttaaaact atttattatt gaccgctata ttatattctg ttaaaatcat
     6241 aaaaaaggta atttttaaat tacctctatt tctttaataa cagaatcttg cgggagagca
     6301 gattatgcct tataacaagt cacctgaatg tcttgatctt gagaaccttc ttggaataac
     6361 agaagagctt aaccatataa aggatataga ctcgcttctt gaccatattt tgcacaatgc
     6421 ccgtcaattt tgcaaggccg aagccggatc aatttttttg agagagaaag atcacctggt
     6481 attcagttat gtacaaaacg atgtacttgt aaaacgcgaa aagaactata acaaacgcat
     6541 atacgacaag tttacaattc cgattaaccg cgaatcaatc gccggatatg tcgccgacac
     6601 cggcaaacct cttaaaatag atgatgttta cgctattgaa agctcagtcc cctacagttt
     6661 taacggctct ttcgacgaaa tgagcaacta caaaactgta tccatgctta caattcccct
     6721 tataacgagc cgtgataaca ttaccggcgt gatacagata attaacccaa aatcggaaaa
     6781 tgacgttaca gtgtttacag agatccattg ccaatacgtg acttttcttg ccaataacgc
     6841 ttctgtcgcg atcgagcgtg cccaaatgac acgcgaaata atactacgca tgatacgcat
     6901 ggccgagctt cgcgacccca aagagacagg tgcgcatgtt aatcgtgttg gcgcctactc
     6961 tatcgaaatt tatgatcgca tcgcaaaaaa acgaggagag tcacccgaaa taattcgccg
     7021 tacaaaagac catttacgta tcgcggcaat gcttcatgat gtaggaaaag ttgcaatatc
     7081 ggatacaatt ctaaaaaaac cggctcgcct aaacgaagaa gagttcaaaa taatgcagac
     7141 ccactgcgcc agcggtgcgg atctttttga aggcaatgtc tcggatctgg acaagctctc
     7201 tacccaaata gcattgaccc atcatgaaaa atatgacggc tcgggatacc cgaacgggct
     7261 cgaaggaaag ctgatccccc tacccggaaa aattgtcgcg cttgccgatg tttacgatgc
     7321 attgatttca aaacgtgttt acaaggaaga gtggcccgaa gaggaggttc tcaaccacat
     7381 aaaatcagag tccggcaaac attttgatcc cgaagttgta gaagcatttt ttgacgttca
     7441 cgacattcta aaagcagttg gtaaaagata ccccgacaag taaaaatatt gacaaaatag
     7501 ctgttttggc ttaatcaaag agacataata tcgagggctg tttatttggc tttttcaata
     7561 cttgtgcgtg tttcgtaatt aaacgatgca atcacaaaat tttaacaaga cttcttgtaa
     7621 caaaaccacg aggcgacaat gaaagagagt attcaaccag agcttattcc ttttgacaga
     7681 atgagagagc aagtcgaaaa aaatctgact acttatatct cacaaagttc ggaatgcctt
     7741 gagctgccaa aaatcaccta tgaaaaagga gtcagtacga tatcctttcc cttgccctac
     7801 tttaacgaaa caaccatctt tacaattata tccataataa aagctcatat cgaaaatgtc
     7861 agaattcact catttgacga tggctattac gcttttcaga tgatgaaccg aaacttgttt
     7921 aataccgagg ggctacttga taatcttaag gtcgaaatat tcggtttaag ctcgggtagc
     7981 cgaacagaaa tatcaaaaaa gggaaacctt agccaggaag agataaattt ttcgatagag
     8041 atatttaaag agctatacga ggtagaacag gataacccgg aaacggcttt acgacgatta
     8101 ggagcctctg tgtatcatga ccatggatct cttgactgga actatatcgc aggttacgaa
     8161 gacgttaaac gcaagataag ggaatcgata ataatgcctc ttcaaaatcc ggacatttac
     8221 gattccatcg cacgactaac ccgacgttca ttcgaaacaa accgtcccag cgcgattctg
     8281 tttgacggcc ctccgggagt tggcaaaacc acaatcgcgc gcataatcgc gggtgaggtt
     8341 aagattcccc ttgtttatgt gcctgtcgaa tcgattatgt caaagtggta cggacagtca
     8401 tcgcaaaacc tctccgccat ttttgataat actgaatttc ttggtagcgc cattcttttt
     8461 cttgatgaga ttgattcgct tgccggatca cgtaactcaa gcatgttcga agcaaccaga
     8521 cgcatattaa gcgttttgct aagacgcctt gacggtatag acagcataag caacacaata
     8581 acaatcggtg ccacaaacag acgcggtgac cttgacccag cgctcatcag ccgctttgac
     8641 cagaccatca gctttccgct tcccgacgcc aaagagcgcg ctgctatatt tagcggatat
     8701 gctcggcata tcgaagagaa agatctcctc tcttttggcg aacagaccgc tggaatttca
     8761 ggacgatcta ttaaagacat ctgcgaatac accgaacgac gatgggcccg gaaaataatt
     8821 atccaaaaag ccgaagccgt cgcgcctccc gcggaatatt acagatacag cattaactct
     8881 tggatgggcg ataactttag cagcacaaca gaaaggggtg atgaagtcgt agagccgctc
     8941 ggctgagtgt aatgtcacga tatatcgtgg cactacacta atcatttaat cgcagtaatc
     9001 tctttttcag aaagacctgt atattttgaa atgttggcaa ctgaaaatcc gtcttttagc
     9061 atagccctgg ctgtttcggt tttaccttct attttgcctt cattccaatt ttcattttta
     9121 attttttcga caagttctgc taacatcggc tttacctcca ttagattacg tattgaatca
     9181 agtttggtat ctttaagctc aacactaaac atatttttta gccagatgaa ataatctctg
     9241 atgtctgacg gatcttcatc tctaatatac attactattc gatcaatggc aatacgcaac
     9301 ttatttttgt ctccggcgtt ttccataagg attacagccg ctataaggtt tgaaagcttg
     9361 tccagatatt tatcagacac ttcgtataaa ataacagcaa aatattcata agacggtata
     9421 aacctgtcgg gcatgttttt atttataaga tcacgaatat tacgtggaac attccatttt
     9481 tttgatccgt tatataacag aagcgggaaa acatttggca ataaacctct tttattattt
     9541 tttagcaaca gatcatataa aagcaggata tactgaaaca ttctgaccgg tatagcctta
     9601 tcaggtgtcg actgaaactc gattaaaata taaatgtagg catctttccc gttaatattg
     9661 atcttgtaaa gcagatcact ctcccgatca agcatatcct gcgagacaaa gctcttatct
     9721 gcaagttcaa gaccttcttc tgttaagttc tttacaaaat cttcatgtac aaatgaatta
     9781 agtaactgta gaaatatcct tttatttgag aacaaatact tgtacgcatt atagtgtggc
     9841 tttgcttttc tattgcattt tctgactatc aacatcctcc ttttttctat ctacatataa
     9901 caactgtttt t
//
LOCUS       DAOTGZ010000085         9904 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_3064
            NODE_8131_length_9904_cov_4.241750, whole genome shotgun sequence.
ACCESSION   DAOTGZ010000085 DAOTGZ010000000
VERSION     DAOTGZ010000085.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868088
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 9904)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9904)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:22:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,785
            CDSs (total)                      :: 2,751
            Genes (coding)                    :: 2,732
            CDSs (with protein)               :: 2,732
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 12 of 19
            Pseudo Genes (internal stop)      :: 5 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9904
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8131_length_9904_cov_4.241750"
                     /isolate="AMR_MDS_3064"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(24..848)
                     /locus_tag="P5123_07075"
     CDS             complement(24..848)
                     /locus_tag="P5123_07075"
                     /inference="COORDINATES: protein motif:HMM:NF018271.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putative ABC transporter permease"
                     /protein_id="HRX16057.1"
                     /translation="MIQKEISTLFITPQLILYFFPIYAFLGWLGEVIYRTITRKQFVN
                     PGLLSGPVLPLYGSASIIIIFIASLFPINNPVFDFIFFAIFCSIIEYMAAALAENYFK
                     IRLWEYDDYRFNLKGRICLRFAIYWGLYTLVFLYIIHPFFYNTFEYITKDVYPEKEHS
                     ITLVLIFINAAIVLDFMISAKAMKKFIITLNKFIENYITLENEQIQQYLYKMRKPLKS
                     FRAIRKYTDFEIRKRLKKNISDQLIGLKQLMSDKLFIKKSSEEEFKSKRLILPIFT"
     gene            complement(1154..2461)
                     /locus_tag="P5123_07080"
     CDS             complement(1154..2461)
                     /locus_tag="P5123_07080"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRX16058.1"
                     /translation="MTRFKLLSTKCIKKTKIIYLTFLTLMITIIISCGGGSSDDKKTE
                     SEDPTPTSTSEIDENLMAYGVPAVDASDNTYMLFKEDLMMSGPTSAVSFKNDGSLNWN
                     VEIDDYLQNIVICNDLIILSGLNKVYAVSKSDGSTKWSYKLTEESGLANTKGVHKPCI
                     DSNGNIVIAVDSYLLDISTAKVACLISIKPDGTLNSKTEFPTGDDYDDRFTKMSPPMA
                     TDSGIFFSVFKTIDTGDSISLYRYDNNLNALNNRPYTGDVASESSFLAVTDTYVLFGL
                     RDNNTYKTNVISMNNTFGSDNWTEILDDYVTNSAVIDKNNNIVISCEDGYIYKLNSTD
                     GSKTWSSNIGKIFVRGELIIGKNQKIYKSGQLPTEVSLSSGSESDPISDAMASSEGAM
                     LSDGTLVYGSDNKLIRVNTSSGGICPDAKWAKYGKDAANSSLY"
     gene            3007..4527
                     /locus_tag="P5123_07085"
     CDS             3007..4527
                     /locus_tag="P5123_07085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005659151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1846 domain-containing protein"
                     /protein_id="HRX16059.1"
                     /translation="MRKIGFDNDKYLEEQSSAILERVRQSSGKLYLEFGGKILFDYHA
                     ARVLPGFDPNVKMRLLQRLSNEAQIILCIYAGDIERRKVRADFGITYDVDAMKLIDDL
                     KEWGVSTPSVVITRYEDQPAANIFKNKLERHGVNVYTHRFTKGYPTNLDLIVSDEGYG
                     ANSYIETDKKLIIVTGPGPGSGKLATCLSQLYHEYRQGKQAGYAKFETFPIWNIPLKH
                     PVNVAYEAATVDIGDFNMIDPFHLEAYGKSVVNYNRDVEVFPVLERILERITGKEAVY
                     KSPTDMGVNRAGFGITDDDIVREASRQEIIRRYMRYACEYALGLADAETVERAELIMK
                     EVKAKPEDRSVYLPAREAAEGASTTGKGHDGIFCGAAIELRDGRIVTGKNSPSMHASS
                     SLILNAVKVLAGVPDDIHLLDPSIVESVTRFKKNILSGTTMSLDLDEALISLVISANS
                     NEAAQKCVDKLTELRGCEAHITHMPTPGDEAGLRRLGVNLTSEPRYSTKGFIEVNQ"
     gene            complement(4532..4753)
                     /locus_tag="P5123_07090"
     CDS             complement(4532..4753)
                     /locus_tag="P5123_07090"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRX16060.1"
                     /translation="MIAIDILTYQFENDEKVTIEIVDNKTISVSGKKNCNMQPLSNKD
                     KELLSMLLESSKCTVVNENKIATVTLGLD"
     gene            complement(5234..5716)
                     /locus_tag="P5123_07095"
     CDS             complement(5234..5716)
                     /locus_tag="P5123_07095"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRX16061.1"
                     /translation="METTDFRTSIDVEINRLERIEDYAKKHNMDISDVMNTVLDKYSK
                     MLAKNPVCNDTLNRYHERGTVYKSVHIRFSSISKRKLKDIANLCCYSLSYMLFLAMEF
                     FDEFMDGVEDFVRPCFHAAFVYHFFGFPYFINSWGMNFYEGQDQPAGQESGKIMNLLC
                     "
     gene            5965..7734
                     /locus_tag="P5123_07100"
     CDS             5965..7734
                     /locus_tag="P5123_07100"
                     /inference="COORDINATES: protein motif:HMM:NF012711.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="long-chain fatty acid--CoA ligase"
                     /protein_id="HRX16062.1"
                     /translation="MPSNVPEIFFETVHTCPHKDALRIQRDDSLLTFSYKECGTMVEK
                     LAKALFNAGIKAEDRVAILSDNMPEWSLSDLALLSIQAVVVPIYQTLPPQQISYILND
                     SSCKAIFVQNSEQYEKIVKIQNEVPSLSLVFSFEKINSSILTFDDLIDGISDDQGAWL
                     SERIAEIEPEKTCSIVYTSGTTGDPKGVELNNAGFVHDIVESERVLTLYQDDVFLSFL
                     PLSHLYERLAGHWCPMYRGGTIHYARSIKTVIEDISIAAPTVMVSAPRLFEKVASAVK
                     DRAASGNILARMIFAWALSVGFRYHEKRASGRAMLLLPLLYKIAWKLVFSKIKKKLGG
                     RFRCPIAGGAPLSVETLKFFEAIDMRIIEGYGMTEAHLIITLTPPGKTRYGSCGKAIG
                     GVSLKIADDGEVLVKGPTVMKGYYKKPELTSEIIDKDGWLHTGDIGYLDDDNYLYLVD
                     RKKNIIVTSGGKNVAPAPIEHRLKKSKYINDICLVGSRRKFISALIIPETTELRRWAE
                     LHGLADATDNELYENDSVYLLYQDEINQLQDGLARFETVKKFQILKEPLSIEKEEITP
                     SMKIKRTVVENNYKDLIDRMYDE"
     gene            8575..8787
                     /locus_tag="P5123_07105"
     CDS             8575..8787
                     /locus_tag="P5123_07105"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRX16063.1"
                     /translation="MKKIISFFMSGLLLLAVTGCGSNETEKAQEARPVGGVLGKEILA
                     LSVELGKAKNIDCAEALKNKDISAEA"
     gene            complement(8817..>9904)
                     /locus_tag="P5123_07110"
     CDS             complement(8817..>9904)
                     /locus_tag="P5123_07110"
                     /inference="COORDINATES: protein motif:HMM:NF033517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="IS66 family transposase"
                     /protein_id="HRX16064.1"
                     /translation="EEIPEIKTAKMPERFIPGSVATEGLVAYSVISKFNDALPFYRQA
                     KIFSRFGVDLSRATLCNWSLLAAKKCTTLLEVLEETILSGEVIRMDETTVQVLHEEGR
                     KAENKSYMWVAYGYGNNEKPLLLYQYHPTRSSDVPKEFLKKYSGFVQTDGYKGYNFTN
                     ASYKGSIIHVGCLAHVRREFEKIYKANRKSKCAHTALKYIRNIYRIESTLRERNLASN
                     EFVTTRKEQMKESLETFHNWLGEQKNIILPASPSGKAISYILGEWKKIICFLDHHLLT
                     PDNNKIENAIRPFVIGRKNWLFSNTPRGARSSACFYSLIESAKANKLVPYYYLRYVFE
                     KLPGCSTKEELRKLLPDMLSPEMIKIS"
BASE COUNT         3132 a         1637 c         1906 g         3229 t
ORIGIN      
        1 gaaagaataa tataatttaa gtgttatgta aagatgggga gaattagacg cttactttta
       61 aactcttctt cgcttgattt cttaataaac aacttatcgc tcatgagctg ttttaatcca
      121 attaactgat ctgatatgtt ttttttcaga cgctttctaa tttcaaaatc ggtatactta
      181 cggatagcac ggaaagactt taaaggtttg cgcattttgt aaaggtattg ctgaatctgt
      241 tcattctcta gggtaatata gttttctata aatttgttaa gagtaataat aaattttttc
      301 atggcttttg ctgaaatcat gaaatctaaa actattgcgg cgttaataaa aattaaaaca
      361 agagtgattg aatgttcttt ttcggggtaa acatcttttg ttatgtattc gaaagtatta
      421 taaaagaagg ggtgaataat atacaagaaa acaagggtgt acagtcccca gtaaatggca
      481 aatcgcagac aaattcgacc tttaagattg aagcggtagt catcatattc ccaaagtctg
      541 attttgaaat agttctctgc caaagcagcg gccatatatt ctattatgga acaaaatatc
      601 gcaaagaaaa taaaatcaaa aacgggattg ttaattggaa ataatgacgc tatgaagatg
      661 ataattattg aggcggaacc atatagtgga agtactggac cggataaaag acccggattt
      721 acaaactgtt ttcgggttat ggtgcggtaa atcacttcgc caagccagcc aagaaaggcg
      781 taaataggaa aaaaatatag aataagttga ggagtaataa atagggtgga tatttctttt
      841 tgtatcatat tgaccacgat taaagttagc tatcttgtta aataaaattg gaaattcatt
      901 atatcagata attaagcaat gctattggta atgtcaaaaa tggtacagca aaataatttc
      961 tttagcgatt tattatgcgg gctaaatgga aagtgttttt aatggttatt ggtaaggttc
     1021 aaacaaattg atgattggga atttgataaa tgtttgggaa aatagaatac ataaaaaagg
     1081 gcttttatat aaagcccctt ttttatgtag ctgtatgtaa tataaacgta tgatcagaat
     1141 tactgacaga aaattaataa agtgaactgt ttgcagcgtc tttgccgtat ttagcccatt
     1201 ttgcatcggg gcagattccg ccggatgatg tgttgaccct gatcaatttg ttatcactac
     1261 cgtaaaccag ggtgccgtcg ctcagcatcg caccttcact tgatgccata gcatcagata
     1321 ttggatcgga ttcgcttccg ctgcttaggc ttacttcagt tggcagctgc ccagatttat
     1381 aaattttctg attcttaccg attattaatt ctccccgtac aaaaatttta ccaatatttg
     1441 aactccatgt cttggatccg tcggttgaat taagtttata aatataaccg tcctcacagc
     1501 tgatgacaat attattattt ttatcaataa cagctgaatt ggtgacgtag tcatcaagaa
     1561 tttccgtcca attatcagag ccaaaagtat tattcatgct gataacattt gttttatagg
     1621 tattgttatc tcgtagacca aaaagtacgt aggtatccgt tacagctaaa aatgatgatt
     1681 ctgaagcgac atcaccggta tatggtcgat tattaagcgc atttaaattg ttgtcatatc
     1741 gataaagtga aatactgtcg cctgtatcaa tggttttgaa gaccgaaaag aatattccgc
     1801 tatctgtagc cattggcgga ctcatctttg taaaacggtc atcataatca tcaccagtcg
     1861 gaaattctgt tttactgttt aatgtgccat caggtttaat tgaaataagg caggcaactt
     1921 tagcggttga aatatctaat agatatgaat caactgcgat aactatgtta ccattcgaat
     1981 ctatacaggg tttatgcact ccctttgtgt ttgctaaacc ggattcttcg gttaatttat
     2041 aactccattt ggtagagccg tcagatttgc taaccgcgta taccttgtta agaccggata
     2101 aaataatcag atcattgcaa ataacaatat tctgcaaata atcgtcaatt tcaacattcc
     2161 agtttagaga gccatcattc ttaaatgata cagcgcttgt tggcccgctc atcataagat
     2221 cttctttgaa gagcatatat gtgttgtcgc ttgcatcaac cgcgggtaca ccgtaggcca
     2281 taaggttttc atctatttcg cttgtgctcg ttggcgtagg gtcttcgctt tcggtttttt
     2341 tgtcatcaga gcttccacca ccacatgaaa taattatagt aatcattagt gtgagaaaag
     2401 ttaaatatat gatttttgtt tttttaatac atttagtgct caataattta aatctagtca
     2461 ttttttacct ctcggtttgt aattattcat tatgcttctt tcttaaataa ttttaaatag
     2521 tgttaattat aagacataaa atttactgaa taatccattg cggattatta tgaaaggtgt
     2581 gaggataaaa aaactgaaat atttatagaa tattatattc cagtataaaa ttgtagtaat
     2641 aaaatttacg ttgtgatcta ttttcaatat gtatgcgttt ataggttagg ttatattgaa
     2701 aacaggtaaa ataaaaaggt tataaatatt atagacaacc actattttat tttaaacgac
     2761 atgcaactgt tttcttgaaa aaaatgttgg tttttttagt tttaaacaaa attccagaat
     2821 ataaataaca taattgtcta ggtgtgtcgt gttaaagtat tagacagtct tttaaaataa
     2881 ggatgtagtt gcatatatag atattaagaa catgacctca gcgtcctttt taatttcagt
     2941 ttatgaaaaa tcttgcattc cagatgtata ctaactaatg tggcatttta ctatggaggc
     3001 taattgatga gaaaaatcgg gtttgataat gataaatatc ttgaagagca gagcagtgcc
     3061 attctggaga gggtaaggca atcttccgga aaactttatc ttgagtttgg tggaaaaatt
     3121 ctttttgatt atcatgctgc aagggttttg cccggttttg atcccaatgt gaagatgagg
     3181 ctacttcaaa gattaagcaa tgaagctcaa ataattcttt gtatatatgc cggagatatt
     3241 gaacgtcgta aggttagggc cgattttggt ataacctatg atgttgacgc tatgaagtta
     3301 atagatgatt tgaaagaatg gggggtaagc acgccttcag ttgttattac tcgttatgaa
     3361 gatcaacccg ccgcaaatat ctttaaaaat aagcttgagc gccatggtgt aaatgtttat
     3421 acccaccgat ttacaaaggg atacccaact aatcttgatt tgattgttag tgatgaagga
     3481 tacggagcaa acagttatat cgagaccgat aaaaaactta tcattgttac cggacctgga
     3541 cctggttcag gtaaattggc aacatgcctt tcgcaacttt accatgagta tcgacagggt
     3601 aagcaggctg gatatgcaaa atttgaaact tttccaattt ggaatatacc gcttaagcat
     3661 ccggtaaatg ttgcctatga agcagctaca gttgatatag gtgactttaa tatgattgat
     3721 ccattccatc ttgaagcgta tggtaaatcc gtagtaaatt ataatcgtga tgttgaggtt
     3781 tttcctgttt tggaaagaat actcgagcga attacgggaa aagaggctgt atataaatca
     3841 cctaccgaca tgggggttaa tcgagccgga tttggtatta cagatgacga tattgtcaga
     3901 gaagcatcac gtcaggaaat tatcagacga tatatgcgat atgcctgtga atacgcccta
     3961 gggcttgctg acgccgagac agttgagcga gccgagttga taatgaaaga ggttaaggca
     4021 aaacccgaag accgctctgt ttatcttcct gccagggaag ctgccgaggg tgcatcgaca
     4081 acaggaaaag gacatgacgg aatattttgt ggtgctgcaa tagaattaag ggacggaaga
     4141 attgttacag gtaaaaattc tccatcgatg cacgcatcat cgagtcttat tttaaacgct
     4201 gttaaagttc ttgctggggt tcctgatgac atacatcttc ttgatccgtc tattgttgaa
     4261 tctgtaacaa ggttcaaaaa aaacatacta agcggtacaa caatgagtct tgatcttgat
     4321 gaggcattga taagtctcgt gataagtgca aatagcaatg aagctgcgca aaaatgtgtc
     4381 gataagctta ccgaacttag aggttgtgaa gctcatatca cacatatgcc cactccagga
     4441 gatgaggcag gtttgcgtcg cctaggtgtt aatttgacaa gtgagccgcg ctattcgact
     4501 aaaggattca ttgaagtaaa tcagtgatat attagtcgag gcctaatgta acagttgcaa
     4561 ttttgttttc gttaactacc gtgcatttag aagattcaag caacatcgat aataattctt
     4621 tgtctttatt agaaagtggt tgcatattgc aatttttttt gcctgagaca gatatggttt
     4681 tgttatctac tatttctatt gtaacttttt catcattttc gaattggtaa gttaaaatat
     4741 ctatggctat cattattgaa actgtttttt caattggtaa tattaaattt tggcttttgt
     4801 taaaattagt aatacagact ttttcggtct atctaccaat ttatttcgtt tatttaactc
     4861 ttttaaagat agataaaaaa tcagaagact ggttattgtt acgaatgcta ttccctttaa
     4921 tatagaaaag aaaacaattg attcagcttg aggggatatc ataagaataa ttttatcaga
     4981 cgaaataatc catagtattg aaataataaa ataaggacat gcaattcggc tagctccttt
     5041 catgttacct gccttagtta ttgtttgaat tgatgtgcaa ttcctcggat ttttagaatt
     5101 ttttattcta gtttaactga ctaaaaataa tttgttttag tcagttaagc aaatttttta
     5161 ttgaaattat aggaatttag ttttttattt gtttttcacg gtttattttt tttgaatcaa
     5221 tcaaaaatgc catttaacac agtaaattca ttatttttcc ggattcttgt ccagcaggtt
     5281 ggtcttgtcc ttcgtaaaag ttcattcccc agctgtttat gaagtatgga aacccgaaaa
     5341 aatgataaac aaaagcggca tgaaaacagg gacgtacaaa gtcttcaaca ccatccataa
     5401 attcatcaaa gaactccatt gccagaaaca acatgtatga caagctgtag cagcagagat
     5461 tggctatatc tttcagtttt cttttagaaa tacttgaaaa ccgtatatgt actgatttat
     5521 aaactgtacc tctttcatga tatcggttaa gagtgtcatt gcatactgga ttcttagcca
     5581 gcatttttga atatttgtcc agaactgtat tcataacatc ggaaatatcc atgttatgtt
     5641 tcttagcata atcctctatt ctttctagtc tgttaatttc tacatcgatc gatgttctaa
     5701 aatctgttgt ttccataagt atcctccaga ttattgtata catgtgtata ctggagtgat
     5761 ggctaaattt ttaaattttt tttaaaaaaa tttacatttg ataagaatta tatataaacg
     5821 aatgaaaacc tgcttgaata cccttatttg cctataaata actccaaaat agattaacgt
     5881 aaaatatttg attccccttg acgtttttta tggtataaat aactgtagtt ctgctgctta
     5941 cattctgtat caagggagat accaatgcca tctaatgtgc cggaaatatt ttttgaaaca
     6001 gttcatacct gtccccataa agatgccctg cgaatacaga gagacgattc tcttctcact
     6061 ttcagttata aagagtgtgg aacgatggtt gaaaagttgg ctaaagcact atttaatgcc
     6121 ggaataaaag cggaagaccg tgtagcaata ttatcggata atatgcccga atggtccctc
     6181 agtgacttag cactgctttc aattcaggct gttgttgttc ctatttatca gacattaccg
     6241 cctcaacaaa tttcctatat tttgaatgac tcgtcatgca aagcaatctt tgtgcaaaat
     6301 tccgaacagt atgaaaagat agtaaagata caaaatgaag taccaagcct gtctctcgtt
     6361 ttctcttttg aaaagattaa ttcatccatt ttaacctttg atgaccttat agatggtata
     6421 tcagacgatc agggagcctg gttatccgag agaattgccg agatagagcc agaaaaaaca
     6481 tgtagcatag tatatacttc aggaacaaca ggtgacccaa aaggggtaga gcttaacaat
     6541 gcaggttttg tgcacgatat agttgaatca gagcgtgtac tgactttgta ccaagatgac
     6601 gtattcctct cttttctgcc gttgtcacat ctttatgaac ggctagccgg tcactggtgt
     6661 cccatgtata ggggcggtac catacattat gccagaagta ttaagacagt cattgaggat
     6721 atatcaatag cagcaccaac tgttatggtc tcagctcctc gactgttcga aaaagtggct
     6781 tctgcggtta aagatagagc ggcatcagga aacattcttg cgcgaatgat tttcgcatgg
     6841 gcattgtcgg ttggattcag atatcatgaa aagcgggctt caggaagagc catgctgctt
     6901 cttcctcttc tttataaaat tgcgtggaag cttgtattct caaaaataaa aaagaaactg
     6961 ggggggcgat tccggtgtcc aattgctggg ggagcacctc tctctgtcga aacattgaaa
     7021 ttttttgaag ccatagacat gcgtattatc gaaggttatg gaatgaccga agcccatctt
     7081 attattacac tgactcctcc gggcaagacc cgatatggtt catgcggcaa ggctataggt
     7141 ggggtatcac ttaaaatagc tgatgatggt gaggtcctag taaagggacc taccgttatg
     7201 aaggggtact ataaaaaacc ggaattgaca tctgagatta tcgataaaga tggatggcta
     7261 catacgggtg atataggata tctggatgat gacaattatc tctatttggt tgaccgtaaa
     7321 aaaaatataa tagttacatc tggtggaaaa aacgtggcac ctgccccaat agaacaccgg
     7381 ttgaaaaaga gcaaatacat aaacgatatc tgtttggtag ggagccgcag aaaattcata
     7441 agcgcattaa ttattcccga gacaactgaa cttcgcagat gggctgaatt acatggctta
     7501 gccgatgcaa cagataatga actttatgaa aatgattcag tttacctttt gtatcaggat
     7561 gagatcaatc aacttcaaga cgggcttgca cgctttgaga ctgttaagaa atttcaaata
     7621 ctgaaggaac cgctttcaat cgaaaaggaa gagatcactc caagcatgaa aataaagaga
     7681 accgtagttg aaaataatta taaagacctg atcgacagaa tgtatgatga ataactgatc
     7741 aaaaaatatt atatccaata tcgtaattga ttctttggct taatttagaa ttgagttgta
     7801 gtatccgttg gatacctccc tttaggcgta tcgatttttc tatcacgatg ctgttgaatc
     7861 gaattgcgtt aagtaaaagc ctttatcacc agttaattta taaagtaaac ctcctacaat
     7921 aaaacgaaat aataaaatgg catattttcc ccggattgat atctgattaa aattcactgt
     7981 ttttcttatt ctatgaaatt agttttaaaa aacttgtcta ccagagcatg actgcaatga
     8041 gatcagcagg taagtgcagg catatcactt ttgttaaaat accactttat atgaattgtt
     8101 aaattattca atttttttaa aattattttt tcaatcaaga aacgaaaata tcgttatata
     8161 gtgttttaaa acaaataact acattagaaa atgagctact attaatagat ggctgtaaat
     8221 tgttagcttc tatgtttttg taaaagagtt attcattaca taaaaaattg taaataaaac
     8281 ttctttactc gtactaaagt gctaaaatta tcaatcgtac tttagttgta cttttgaaaa
     8341 aaaaaatgaa ttttacagaa aaagtaaatt atccaagtat ttatctggtt catgccaaga
     8401 gactgttata aatttaatta cagcttaata tactagttac atgtgctaaa aaaatacttt
     8461 cataagatgt ttatttagtg ttgtgtgaca tgattttaaa ctgatttgta aaatgtccgg
     8521 aagcaagagc tgatttttat tagatgttta aaaaaacaaa ggaaggtcgt tgttatgaaa
     8581 aagattattt cattttttat gtcaggttta ttgcttcttg ctgtaactgg atgcggttca
     8641 aatgagacag aaaaagctca ggaagccaga cctgttgggg gtgtcttagg caaagaaatt
     8701 cttgctttaa gtgtagagct tggaaaagca aagaatattg attgtgctga agctctcaag
     8761 aataaagata tttctgctga agcgtaagcg tcaatttccc agcaggttgt ctccggtcag
     8821 gatattttaa tcatttcagg tgagagcata tccggcaaga gtttccgcag ttcctctttt
     8881 gttgaacatc ccggcagttt ttcaaaaaca tagcgtagat aataatatgg taccagtttg
     8941 ttagcctttg cactttcaat tagagagtag aaacaggcac tgcttcgcgc tccacgagga
     9001 gtatttgaga agagccagtt tttgcgccca atgacaaatg ggcgaatggc attttctatt
     9061 ttattgttat ccggcgtcag cagatgatga tcaagaaaac agattatttt cttccactct
     9121 ccaagtatat aggatatggc cttgccactt ggacttgcgg gtagaataat attcttctgc
     9181 tcaccaagcc agttatgaaa cgtttcaagg gactctttca tctgctcctt ccgtgtagtc
     9241 acaaattcat tacttgcaag atttctctca cggagagtac tttctatccg gtagatattt
     9301 cgtatatatt tgagagctgt gtgtgcgcac tttgatttcc tgtttgcctt gtatatcttt
     9361 tcaaattccc tgcgaacatg ggcaagacag cctacatgga taatcgatcc tttataggag
     9421 gcattggtaa agttatatcc cttgtatccg tcagtctgta caaaaccgga atattttttc
     9481 aggaattctt tcggcacatc ggatgagcgt gtcgggtgat actgatataa taagagcggt
     9541 ttctcgttat ttccatagcc gtaggcaacc cacatgtatg atttgttttc cgctttacgg
     9601 ccttcttcat gcaagacctg cactgtggtt tcatccatgc gaatcacttc tcctgaaaga
     9661 atcgtctctt caagaacttc aagcaatgta gtacattttt tcgcggcaag aagcgaccag
     9721 ttacataacg tcgcacgtga aagatcaact ccaaagcgtg agaaaatttt tgcttgtcta
     9781 taaaacggca acgcgtcatt aaatttcgat atgacagagt atgccacaag cccttctgtt
     9841 gcgacacttc caggaatgaa acgctccggc atctttgcgg ttttaatttc aggaatttct
     9901 tctt
//
LOCUS       DAOTGZ010000086         9876 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_3064
            NODE_8155_length_9876_cov_4.774870, whole genome shotgun sequence.
ACCESSION   DAOTGZ010000086 DAOTGZ010000000
VERSION     DAOTGZ010000086.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868088
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 9876)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9876)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:22:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,785
            CDSs (total)                      :: 2,751
            Genes (coding)                    :: 2,732
            CDSs (with protein)               :: 2,732
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 12 of 19
            Pseudo Genes (internal stop)      :: 5 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9876
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8155_length_9876_cov_4.774870"
                     /isolate="AMR_MDS_3064"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(132..707)
                     /locus_tag="P5123_07115"
     CDS             complement(132..707)
                     /locus_tag="P5123_07115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013655518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6434 domain-containing protein"
                     /protein_id="HRX16065.1"
                     /translation="MRPELNKNSNADEFLDFYWLKEELVIFCKNQDLPSCGSKDELTA
                     RIHEYLRTGKVIIPVKNKSEAEQKTGAPPALGSKIPAGYKNDEMHREFFKSVIGNHFK
                     FNVAFMNWMKGNHGKTYQDAVLEWNRIIDEKKQGKKTEISSQFQYNQYTRDFFNANPK
                     AKRDEAVKCWKYKKSLPGHNKYEESDLVALK"
     gene            complement(865..1500)
                     /locus_tag="P5123_07120"
     CDS             complement(865..1500)
                     /locus_tag="P5123_07120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009170283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRX16066.1"
                     /translation="MIKKLAYSVGRKDEKPNIELAVELTENEDLKGIAEIVNGLNDSV
                     EQIANDCIKVLYEIGERKPQLIAEYVDIFIQLLNSRNNRLVWGAMTALSKIAFLKPQR
                     IFDNLEIIIKAYEKGSVITVDNSISVFAALAKAGPEFNKVVFPLLIKHLENCRPKEVA
                     QHSERAFVCIDSKNKEDFIDVLNKRYDSLTDSQKKRIAKIIKRTKEERYLL"
     gene            complement(1735..2565)
                     /locus_tag="P5123_07125"
     CDS             complement(1735..2565)
                     /locus_tag="P5123_07125"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="transglutaminase"
                     /protein_id="HRX16067.1"
                     /translation="MNKINQKDVLNYYRQYSSNTDPGEYLFLYESLPVDIYELCVLIK
                     KQLIHPVRIDEFPELAGKKSEDETFHSVREMLEELVKRNPKGLVIDRLPEERLRLSCR
                     FHSLLLASILKSQNIPVRVRVGFAGYLAPPGVNKSIDHWICEVWDEQKSSWIYVDPDL
                     CKVNFDKNEFEPAGESWLKVQKKQVDPNQYGVFIWWGHDYIKANMLHDFSCALNNELI
                     YWEGPVLARKKLFSYTTDDLELLDTISVLLQDIDDNFYELKKISLDKRLGNITDYPRY
                     "
     gene            complement(2775..3575)
                     /locus_tag="P5123_07130"
     CDS             complement(2775..3575)
                     /locus_tag="P5123_07130"
                     /inference="COORDINATES: protein motif:HMM:NF024626.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4037 domain-containing protein"
                     /protein_id="HRX16068.1"
                     /translation="MKSFKKLLQEQSPEVKQLRDMAAEIVNEKIAHEQMILGTLLTGS
                     VARGDARIGPLGVMIDLTIVTKTVNDVSLEEIFGADEEPFIPSHCVTLHDNIGLAVQT
                     IEENELWKIRTKDEPSIFAMNESVVLNDKKGILKKWKEEYFEITPDQVKERALNHYFR
                     YCYLTNDYRFEKWSYRKAWEQIAQNFNEANECYCNFLYCINDMFIPRKDWLAYLTYEM
                     KIRPENHRQYMAEIYEMQLNDKLIKQKCKTYQAIERWMSSYIKGRSWL"
     gene            complement(3725..4252)
                     /locus_tag="P5123_07135"
     CDS             complement(3725..4252)
                     /locus_tag="P5123_07135"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024812.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HRX16069.1"
                     /translation="MVSIRVSENLCLEIVTHCDCDKIFSLVEKNRAYLAEYMSWVETT
                     TSTDDIKTFINSCFDQLKNMSGICYKILVDGETAGTIGFFVTNEKTATFEIGYWIAQK
                     YSGRGIVSRLVPIVEKICSEQYKAGKIEIRCATENSASNRVAQKNNYEMEGTIRRCEI
                     VNKKYLDYNVYGKCL"
     gene            complement(4517..5407)
                     /locus_tag="P5123_07140"
     CDS             complement(4517..5407)
                     /locus_tag="P5123_07140"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRX16070.1"
                     /translation="MIYKKIKNKINPEKLIYKILFEFFKVILIVFFTLMFTFIGMLFI
                     GIVGLPVLFVIIIIGIIFFVRYLLKNRKKPDIKTYLGFGYNLVLIFLIVMIPIYIGIF
                     VVSGIEDITQNNFIVVDPDYVEENLININIRIRNSGKPEEIVTRKFPYTKHVKKIYPD
                     LFNLQIAYHFNNYNISKIKINKYFFIINGKEYNIYDKDFDIFGSVYYKDIDSNRSSDT
                     ELTQEEIESYKKTKEMLLIRSKNEVISGIICNFRDLEINAEKIDKIIFEYDIDLIMED
                     GEIHNINKRNELYKEEKAVY"
     gene            6011..6985
                     /locus_tag="P5123_07145"
     CDS             6011..6985
                     /locus_tag="P5123_07145"
                     /inference="COORDINATES: protein motif:HMM:NF013567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HRX16071.1"
                     /translation="MNYTVIYGFTPAISNTVLKGVYLNIGIVGSGFMGSMHANLYKKI
                     KCAHNQGITNKSSTNAKKIADDLSIQCYQDYKKMLNDSAIDIVDICTPTDSHTLIAIE
                     SMKMNKHVILEFPAVSSLSELEELRKVSRETGKICALAYYSRFQSQYKYIFDAAISGS
                     LGKINGLSISRKSSPVFYSDDIVNNLISQDIDFMVRLLGKPNKIHASNIKQKCAVFIF
                     EYDCLSAVISGAVNMHKNFPFSTHHHVSGKKGSLDLSWKFTDHPEYTMTQTSENGSSN
                     INNQDYDPYHKELEVIVEGIINNQTSAFDIESAAMGITLSLECRELLR"
     gene            complement(7270..8247)
                     /locus_tag="P5123_07150"
     CDS             complement(7270..8247)
                     /locus_tag="P5123_07150"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014370838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HRX16072.1"
                     /translation="MEKRKLGRSGIEVSALGLGCWAIGGPFILGGKADGWGQVDDEES
                     IRAIHKALDLGVNFIDTADCYGVGHSEEVIGKALNGKRDKAVIATKFAHFGNEATKTL
                     HGVNLTPDYIERACDASLKRLQTDYIDLYQLHEWNVALVEVDPILDALDRLVQKGKIR
                     TYGWSTDLVNGALLFSQRENCSAIQHCMNIFQDASEMVNFCEKNDLASINRSPLAMGF
                     LTGKFKSDSVVAKDDVRGAGHSWVPFFKDGKPEEESLKRLDSVKEILTSDGRTLTQGA
                     ICWLWARSGKTIPIPGFKSVKQIEENAGALEKGPLSSEQMSEIEKILGR"
     gene            complement(8302..8895)
                     /locus_tag="P5123_07155"
     CDS             complement(8302..8895)
                     /locus_tag="P5123_07155"
                     /inference="COORDINATES: protein motif:HMM:NF012655.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HRX16073.1"
                     /translation="MAIKGTDTKERILKEALSEFASHGYEGARMDRIAAAVGINKASL
                     YFHFKSKEEIFRELFNAVLRKYKSFIRRTADSLSGLETGKRLEEFCRIYLEDSFDNPE
                     IDFWNRIYYLPPQSMRAEILSLTKDDKDCMILELIKIIEEGKSKKEVRNIDSKKAALT
                     LYYSLTCVALSKDIMTKKEGLADMKKVLDFYLQSILS"
     gene            9157..9453
                     /locus_tag="P5123_07160"
     CDS             9157..9453
                     /locus_tag="P5123_07160"
                     /EC_number="2.7.7.-"
                     /inference="COORDINATES: protein motif:HMM:NF014021.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase domain-containing
                     protein"
                     /protein_id="HRX16074.1"
                     /translation="MRLSEDILIKIKEYKQLCFPHDSSLYLFGSRTDDTKKGGDIDLF
                     LESNSTVDPKSEIDFLKLIYKNATQRKIDLVVKTPDKKTTAIYQTAKAEGVLLC"
     gene            9447..>9876
                     /locus_tag="P5123_07165"
     CDS             9447..>9876
                     /locus_tag="P5123_07165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015419730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRX16075.1"
                     /translation="MLKNSINTAFLHYNRAKKNHNEIDIDNLNSKVFLDDNKIKTIDA
                     FIYRFIKLQDFMGDKLFKDLLTSLGEYKDNMSLLDVLDKLEKLEIIKSADQWFSFRAI
                     RNKMTHEYPDNEQDVIAGLILAIDAFNIMEEILLSVKKYCR"
BASE COUNT         3087 a         2073 c         1407 g         3309 t
ORIGIN      
        1 gtatttttga caaaatacgg tacaacgaaa agggcaacca ggttttactg gtaaaatacc
       61 ttaataaaac ggattaatca taacatttga attgtttact gtttttaaat atgatacagc
      121 agagtctcct atcactttaa cgcaaccaga tcactctctt cgtatttatt atgtccgggt
      181 aaagactttt tatatttcca gcattttacc gcctcatccc gtttggcttt cgggttagcg
      241 ttaaagaaat cccttgtata ctggttatat tgaaactgcg atgatatttc tgtcttcttt
      301 ccctgttttt tttcatcgat gattcggttc cattccagta ccgcatcctg ataggttttt
      361 ccatgattgc ctttcatcca gttcataaat gcaacgttaa acttaaaatg gttaccaatt
      421 accgatttaa aaaattcacg gtgcatctca tcatttttat accccgccgg tatttttgag
      481 cccagagcgg gaggcgcccc cgttttttgt tctgcttcac ttttattttt taccggaatt
      541 attactttcc cggttctcag atactcatga attctggcag tcagttcgtc cttgctgccg
      601 caacttggca aatcctgatt tttacaaaag atgactaact cttctttaag ccagtaaaag
      661 tctaaaaact catctgcatt actattttta tttaactccg gtctcaactc ttatctccca
      721 ggacaaacaa tttagttact tgctgcaatt tttttacatt acccgcctgt ttatgctatc
      781 cagcaaccaa tttcataaat ttatgtcttt tcattcgtca caaaagaacc gattgttccg
      841 gcaatttcac caccaattaa tattttacag cagataccgc tcttcttttg ttctttttat
      901 tattttagcg atacgctttt tttgagagtc tgtcagggaa tcatatcgct tatttaacac
      961 atcaataaaa tcttctttgt tcttactgtc tatgcaaaca aaagctcttt ccgaatgctg
     1021 agcaacttcc ttgggtctgc aattttcaag atgttttatc agtaacggaa aaactacttt
     1081 gttaaactct ggtccggctt ttgccaatgc agcaaacaca cttatgctat tatcaacagt
     1141 aataacagaa cctttttcgt atgcttttat aatgatttca agattatcga atattcgctg
     1201 aggtttcaaa aacgctattt tggaaagagc agtcattgca ccccaaacta atctgttgtt
     1261 tcttgaattc aaaagctgaa taaaaatatc gacatattcg gcaattagct gaggctttct
     1321 ttcgccaatt tcataaagaa ccttaataca gtcatttgct atttgctcaa cagaatcatt
     1381 aaggccgtta acaatttcgg caattccctt taaatcttca ttttccgtta attccacagc
     1441 caattcgata tttggtttct catcctttct tccaactgaa taagccagtt tttttatcat
     1501 atgattacct gccattatta atactttata tataacaaag aatcacttat acagactcag
     1561 aatattttat acaaaagcaa ttgcatcttt cttacaagct tcatgtccac tgtcatatga
     1621 tcaaattttt ggcatacaac tctgaaaagc cgcatatagc gcaaacatac attcccgaag
     1681 atcggcggtt acatctggca ctttctacgt tttttaattt gatttgtctc tctattaata
     1741 tcttggataa tccgtaatat tacccagtcg tttatcaaga ctgatttttt ttaattcata
     1801 aaagttgtca tcaatatcct gaagcaaaac ggatattgta tcaagcaatt ccaaatcatc
     1861 cgttgtatac gaaaataatt tctttcgggc aagaaccggc ccctcccaat atataagctc
     1921 attatttaac gcacacgaaa aatcatgtaa catattagcc ttgatataat catgccccca
     1981 ccatatgaaa acaccatatt gattcggatc cacctgtttt ttttgaactt taagccagga
     2041 ctcaccggct ggttcaaatt catttttatc aaaattaacc ttgcataaat cgggatcaac
     2101 ataaatccaa ctgctttttt gctcatccca aacttcacaa atccagtgat caatactctt
     2161 gtttaccccg gggggagcca aatatcccgc gaatccgact ctgaccctta ccggtatatt
     2221 ttgtgatttc aaaatagaag caagcaataa tgaatgaaat ctgcacgaaa gcctgagcct
     2281 ttcttccggc aacctgtcaa ttaccaaacc cttaggatta cgctttacca actcttccag
     2341 catttccctg acagaatgga aagtttcatc ttcggatttt tttccggcta attccggaaa
     2401 ttcatctatt cttactggat ggattaattg ttttttaatt aatacacaga gctcataaat
     2461 atctaccggc agactttcgt atagaaacaa atactccccg ggatcggtat tgctgctgta
     2521 ctgcctgtaa taattcaaaa catctttttg atttatctta ttcacgtttt caaaataccc
     2581 tctaccgcga aatatcaaaa taaatacaac aactgtaata ataatcaata ataatatttt
     2641 attttttttt attatgtttt tgaacacgtt agccacctaa atacattata cataatataa
     2701 gattattttc accaacaata tttttattta tctttggtct ctactcttat ttcatgatat
     2761 aagcagtttc gttatcacaa ccagctgcgc ccctttatat aactgctcat ccacctctct
     2821 atggcctggt aggtcttaca tttctgtttt atcagtttat catttaactg catttcgtaa
     2881 atctcagcca tatattgacg atgattttcc ggcctgattt tcatctcata ggttagatag
     2941 gcaagccaat ccttacgtgg tataaacata tcgtttatac aatacaaaaa attgcaataa
     3001 cactcattag cctcattaaa attttgagca atttgctccc aggccttacg atagctccac
     3061 ttttcaaacc tgtagtcatt cgttaaataa caatatctaa aatagtgatt caaggctctc
     3121 tctttaacct gatcaggcgt tatttcaaaa tattcctctt tccatttttt taagatacct
     3181 tttttatcat ttaatactac agattcattc attgcaaaaa tggagggttc atcttttgtt
     3241 ctgattttcc acaattcgtt ttcttcgatt gtctgaactg ccaagcctat attatcatga
     3301 agagtcacac aatgagaagg aataaatggt tcttcatctg ctccgaaaat ttcttcaagg
     3361 cttacatcat ttacagtttt tgttactatg gttaaatcaa tcattacacc caaaggccct
     3421 attcttgcgt cacctcttgc aacactgcct gttaataatg tacctagtat catctgttca
     3481 tgagcaatct tttcattaac aatttcagcc gccatatctc ttaattgttt tacttccgga
     3541 gattgctcct gtaatagttt cttaaaactt ttcacaacat ctcctctacg tatgtcatcg
     3601 aagggttact ccatatccat tatcttttgt accagcaata ttcttgatag tatcaaacgc
     3661 ctattttgtt gaatattcag tctctttatt gttttccatg tttgactgtc acatatcacg
     3721 attattacaa acatttacca taaacattgt aatcaagata ttttttgttt actatttcac
     3781 atcttctaat agtaccttcc atctcataat tgtttttttg agcaaccctg ttactcgcac
     3841 tgttttctgt tgcacagcgt atctcaattt taccagcttt atattgctca ctacagatct
     3901 tttcgacaat tggcacaagc cgcgaaacaa tgcctcttcc actatacttt tgagcaatcc
     3961 agtaaccgat ctcgaacgtt gctgtctttt catttgtaac aaaaaatcca atggttccgg
     4021 ctgtttcacc atcaactaaa attttataac agataccgct catgtttttt aactgatcaa
     4081 aacagctgtt aataaatgtt ttaatatcat ccgtacttgt agttgtttcg acccatgaca
     4141 tatactccgc aagatacgct ctattttttt caacaaggct aaaaatttta tcacaatcgc
     4201 aatgggtaac aatttcaaga caaagatttt cactaactct gatgctaacc atagttcctc
     4261 cggaatatta attttgagca agctggataa ctctatactg tttcggatct ttttgcgaac
     4321 ttttagtttt cagccacacg tctccgacta attcaaactc atttctgtca aaatcagctt
     4381 ctcaaccaaa aatgcttgtg tagttttcat atttccaggc aaaatacaag catacattca
     4441 cagttaaatg tattatttga ggaagcgtat attatttact aaatcataca tattattcca
     4501 aaccggaaaa ataactttaa taaactgctt tttcctcttt ataaagttca ttacgcttat
     4561 ttatattatg tatttcaccg tcttccataa tcaaatcgat atcatattca aaaataattt
     4621 tatctatttt ttccgcatta atttctaaat cacggaaatt acaaataata ccagatatta
     4681 cctcattctt agatcggatt aataacatct cttttgtttt tttataagat tcaatttctt
     4741 cctgagtaag ttcagtatca ctgctgcgat ttgaatcaat atctttataa tatacagaac
     4801 caaaaatatc aaaatcctta tcgtagatat tgtactcttt gccattaatt ataaaaaagt
     4861 acttattgat ttttattttt gaaatattat aattattgaa atgataggca atctggagat
     4921 taaacaaatc aggataaatc tttttcacat gtttagtata tgggaatttt cttgttacaa
     4981 tttcctccgg tttgcctgaa ttcctgattc ttatgttaat atttataaga ttttcttcaa
     5041 cataatccgg atctactaca ataaagttat tttgggtaat atcctcgatt ccagagacaa
     5101 caaatatgcc aatgtaaatc gggatcataa cgatcaaaaa aattaaaacc agattatatc
     5161 caaatcccag gtaagtttta atatctggtt tttttctgtt tttcaaaagg tacctgacga
     5221 aaaaaataat ccctattata atgatgacaa aaagtacagg caggcccact atccctatga
     5281 aaagcatacc aataaatgta aacatgaggg taaaaaatac aatcaggata actttaaaaa
     5341 actcaaagag gatcttgtaa attaactttt ctgggttaat cttgttctta atctttttgt
     5401 agatcaagaa agaccatagt attgaagcaa gatatgcgaa aggaataacc acaaatatag
     5461 ttgtaaatgc cattattaat ccaccttaaa aaatattaca atgaataatt gaatcaaacg
     5521 ttccataaat aagcgaaacc agccgccggc cagtttgtac agaatacgga tccattatca
     5581 aatctgttat acaatcacaa ttattataat ataattattt ttttcttaaa atatgcatca
     5641 actttttcca aagattaatg ttccaaatta tcgcactttt tttttaacaa aatttcagat
     5701 aaatacgcaa gtacacgcca atgtaccttg taccgactaa gttttccact agtaaaataa
     5761 attgacagcc tttaaattag gcccatttat tcgatattat ccaggtaaaa gcttctattt
     5821 atgtgattct tcagaatttt caagtttctg gtgggccgac tgaaaatata attattatta
     5881 tataacaatt atattttctg aacaatttga acagtttaaa acttttatat aaaattatgc
     5941 gaaatatggc ttgcaacaat tcaatctgtc gcaaattacc aatagttctt tacatgaaat
     6001 caaattttat ttgaattaca cagttatata tggatttaca ccagccatat caaatactgt
     6061 tttaaaagga gtttatctga acataggtat tgtcggctcc ggttttatgg gttcaatgca
     6121 tgctaattta tataaaaaaa tcaagtgcgc acacaaccaa ggcatcacca ataaatccag
     6181 cactaacgca aaaaaaattg cagatgatct ttcaatacag tgctatcagg attataaaaa
     6241 aatgcttaat gattctgcta tagatattgt ggacatttgc acgccaacgg actcccatac
     6301 tttaatagca atcgaaagca tgaaaatgaa taaacatgtc attcttgaat ttcctgctgt
     6361 tagcagcctt tcagagctgg aagaacttcg taaggtaagc agagaaacag gaaaaatttg
     6421 cgctcttgcc tactattcac ggtttcaaag ccaatacaaa tatattttcg atgctgccat
     6481 ttccggctca cttggaaaaa ttaacggcct gtcaatatcc agaaaaagtt cacctgtttt
     6541 ttactcagac gacattgtaa ataatttaat aagtcaggat atagacttta tggtacgcct
     6601 gctcggtaag ccgaataaaa tccatgcctc aaacataaaa cagaaatgtg ctgtttttat
     6661 ctttgaatac gactgtttgt cagccgtgat ctcgggtgct gttaatatgc acaaaaactt
     6721 tcctttttca actcatcatc acgtttcagg caaaaaaggt tctcttgatc tctcatggaa
     6781 atttacagat catcctgaat acaccatgac tcaaacttct gaaaacggca gcagtaatat
     6841 taacaatcag gactacgatc cctatcacaa ggaactggaa gttattgtcg aagggattat
     6901 aaataaccaa acatcggctt ttgatattga atctgccgca atgggcataa cactaagcct
     6961 tgaatgccgg gaactgttaa gatagaaaac ccgaaatact taatcgaaat ccggcattgt
     7021 ttccaaaagc gaaaccaaat atctttaccg ggtataacag aaaatgatat tggataattc
     7081 ttcacagcga agtccatatc cggattaatg tctgatttta ttattatata atcatgtttt
     7141 acctttatgt tgattacgta caattttcca taaaaataaa gacgcacttg aatatcagta
     7201 ttcgctaata tcaacgtgca tctttatctt tcaattaata aacaactttc ctgttaaaac
     7261 tttgcctctt taccgtccca ggatcttctc tatctcgctc atctgttcac tgcttaaagg
     7321 tcctttttct aaagcgcctg cgttctcttc gatctgtttc acgcttttga atccgggaat
     7381 aggtatggtc ttgccgcttc ttgcccatag ccagcatatt gcaccctggg taagagtccg
     7441 tccatcagaa gtaagaatct ctttaaccga atcaagccgc ttaaggctct cctcttccgg
     7501 ctttccatct ttaaaaaacg gaacccacga gtgacccgcg ccgcgaacat cgtcttttgc
     7561 aacaaccgaa tcggacttaa attttcctgt taaaaatccc attgcaagcg gactgcggtt
     7621 aatcgaagca agatcatttt tttcacaaaa gtttaccatc tcagatgcgt cctgaaaaat
     7681 attcatgcag tgctgtatgg cgctgcagtt ttcacgctgt gaaaaaagca gagccccgtt
     7741 tacaagatca gtactccacc catacgtgcg aatcttgcct ttctgtacaa gccggtctaa
     7801 agcatccagt atcgggtcca cttcaaccaa agcgacgttc cattcatgta actggtacag
     7861 atcaatataa tcggtttgta atctttttaa cgaagcatcg caggctcgtt cgatgtaatc
     7921 gggagtcagg ttcacaccat gcaaagtctt tgtagcctca tttccaaaat gggcgaattt
     7981 ggttgctatc acagccttgt ctcttttccc atttaaggcc ttccctatta cctcctcact
     8041 atgaccaact ccataacagt ccgcggtatc tatgaaattt acacccaaat caagtgcctt
     8101 atgaatcgcc ctgatcgact cctcatcatc aacctgaccc cagccatcag cctttccgcc
     8161 caagataaac gggcctccga ttgcccagca acccagaccc aatgccgata cctcaatacc
     8221 gcttcttccc agtttacgtt tttccattta tttctcctat tttctaccta ccgtttggta
     8281 ggttaatatg caaaaaaaat attagctcaa aatactctgc aaataaaaat ccaagacctt
     8341 tttcatatcg gccaaaccct ctttttttgt cattatatct ttagacaacg caacacaggt
     8401 aagcgaatag tatagcgtca acgcggcttt tttgctgtct atgtttcgca cctctttttt
     8461 actcttgccc tcttcaataa ttttaatcag ctccaaaatc atgcaatcct tatcgtcttt
     8521 tgtcagactc aatatttcag cacgcatact ttgtggtggt aaataataga ttctgttcca
     8581 aaaatcaatt tccggattat caaaactgtc ctcaagatag atacggcaaa actcttccag
     8641 tcttttgcct gtttcaagtc cggaaagcga atcggccgta cggcgaataa acgacttgta
     8701 tttacgcagc accgcgttaa aaagctcccg gaatatctcc tctttgctct taaaatgaaa
     8761 atagagagaa gccttattaa tacctacagc ggcggcaatc ctgtccatcc gggccccttc
     8821 gtatccatgc gaagcgaatt cgctcaaagc ctctttcagt attctttcct tagtatcagt
     8881 gcctttaata gccataacaa cctaccaact gtttggttag cttacaaaat caatgcttaa
     8941 atgtcaacaa aaatgatatt tttcttctta tttcaagtac aaactgaaaa attccagcaa
     9001 ttctttatta aaaccaagta tttttccaat acattttgag tatcaatgag ccatactgca
     9061 ccctcccccc ttgctcattt gacttgacat ggagcaatct cagggttata gtaaaagtcg
     9121 tctttaataa tgatttctta aaaaaggact taataaatgc gtttatctga agacatatta
     9181 atcaaaatta aagaatacaa acaactttgt ttcccgcatg attccagcct ctatctcttt
     9241 ggcagtcgca cggatgacac aaaaaaaggt ggcgacatag atctttttct ggagagtaat
     9301 tcaactgttg atccaaagtc agaaattgat tttttaaaac ttatatacaa aaatgcaact
     9361 caacgaaaga ttgatcttgt tgttaaaacg cctgataaaa aaactaccgc tatttatcaa
     9421 acagcaaaag ctgaaggggt gctattatgc tgaaaaattc gataaatact gcctttttac
     9481 actacaatag ggcaaaaaaa aaccacaacg aaatcgatat agataatcta aatagtaagg
     9541 tttttcttga tgataacaaa atcaaaacca tagacgcttt tatttacaga tttatcaagc
     9601 ttcaagactt tatgggagat aaacttttca aagatctgtt gacatccctg ggtgagtaca
     9661 aagacaacat gtctttactc gatgttctgg ataaattgga aaaacttgag atcatcaaat
     9721 cagcagacca gtggttcagt tttcgagcca ttcgaaacaa aatgactcac gaatatcccg
     9781 ataacgaaca agatgtaatt gccggattga tacttgccat tgacgctttt aatattatgg
     9841 aagagatatt attatcagta aaaaaatatt gccgca
//
LOCUS       DAOTGZ010000087         9872 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_3064
            NODE_8160_length_9872_cov_6.798003, whole genome shotgun sequence.
ACCESSION   DAOTGZ010000087 DAOTGZ010000000
VERSION     DAOTGZ010000087.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868088
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 9872)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9872)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:22:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,785
            CDSs (total)                      :: 2,751
            Genes (coding)                    :: 2,732
            CDSs (with protein)               :: 2,732
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 12 of 19
            Pseudo Genes (internal stop)      :: 5 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9872
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8160_length_9872_cov_6.798003"
                     /isolate="AMR_MDS_3064"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(48..557)
                     /locus_tag="P5123_07170"
     CDS             complement(48..557)
                     /locus_tag="P5123_07170"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRX16076.1"
                     /translation="MINRKILSVLFLFLPVFFVNASCEIDDSVRDMNGTEHEFEGRLW
                     KIERYEYKDGSEFFYPVDEAGIEVYHYHSYADGIFRQYESYHYPDGEIFNFYNSRIYA
                     EYEKVGEEYWFSNGEKWNCAVEDDLAICVCSETPDISVIAIVYERSSLTAEDFNSKKS
                     LRTADLSFQ"
     gene            complement(558..1892)
                     /gene="radA"
                     /locus_tag="P5123_07175"
     CDS             complement(558..1892)
                     /gene="radA"
                     /locus_tag="P5123_07175"
                     /inference="COORDINATES: protein motif:HMM:TIGR00416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RadA"
                     /protein_id="HRX16077.1"
                     /translation="MAKKKTVFVCVSCGAESGAWSGKCLQCGEWNSIVEADTAPSKEA
                     KATAVSLSQVNSDDVIRLKSGYREFDLVCGGGIVKGAVTLIGGEPGIGKSTLSLQIAN
                     SFKTLYVSGEESPVQIRQRADRLDIKKDNIMITTCTSVEGIEELIEKHKPECVFIDSI
                     QTITSANMSAPAGSASQLRDSAIRLTAVAKKRNIPLFLIGHITKEGSIAGPKILEHVV
                     DTVLYFEGDFNKEFRILRSFKNRFGSVNEIGLFTMTDRGLSEVKDKNSVFFSDDCFGS
                     PGVAVGSAIEGSRSILFEVQSLVSYTSFANPRRTADGLDFNRLVIIAAALERYAELKL
                     SNYDIFINLAGGFSINETASDLAVALSIASGYSNRSLPDRTGFLGEISLAGEVRPVSQ
                     FERRITEFERNNFSAVYVPAKNIKDKNDLKVSLSIVPVKTIQEAIKKAFGEG"
     gene            complement(1897..2772)
                     /locus_tag="P5123_07180"
     CDS             complement(1897..2772)
                     /locus_tag="P5123_07180"
                     /inference="COORDINATES: protein motif:HMM:NF014523.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MlaD family protein"
                     /protein_id="HRX16078.1"
                     /translation="MKITTEAKVGMMITFSFTLFIVIVALLAQYNINRSGYNIRLYFT
                     FLNDLKVGSSVKIGGGIDIGHVVEINQSQDKTEVKIWIDNNYRLSKSTSFAIYTTGLI
                     GEKYINVIVPAVPDDTGYLADGDIKYGIDPASFDRMMQTFQSFLRDQDGGEILADIFL
                     NSSKFVENMNDIVEDNKYDVKSSVVMAKSTIENLNVQTRELMFNVNKLAKNMSDISTD
                     NKADIDSTLKNLSQTTASLNKLVYRLESGRGTVGRLMYDEDIYNNLRDASIYARDLFY
                     DLKQDPSKLFFKSSR"
     gene            complement(2808..4577)
                     /locus_tag="P5123_07185"
     CDS             complement(2808..4577)
                     /locus_tag="P5123_07185"
                     /inference="COORDINATES: protein motif:HMM:TIGR02231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mucoidy inhibitor MuiA family protein"
                     /protein_id="HRX16079.1"
                     /translation="MKIIGIIMVIFFFSDCIMSAPLVSRIDSVKLFQNRGEIERIVDV
                     QLKKGLNRIEIGSLPVNILDWSVKASAGSDVRILSVEIEKFALSTRRRLEIEKLEKQL
                     DLLKNRDREHLDTLLNINSQLKFLDGISSVSAENAKHETVRGVPNSKAWASTLDFIAA
                     RRKSLYLQKRSVEQKRIELGKEIRKWEYELSKIGGSNYYGLYQRLNKSAYDNASGEQI
                     QKFADMTDVYEKRNIYLVTPVSENDYEKRAVIEINSPGKIKTRLRIKYLIPGTAWKME
                     YDIRAQSVSGKVEMALYANLFQQTGENWENVKMSISTGSPAGAISVPVFYPWFLDIER
                     KREESSGAVEEYEVKRAIPMSERAAKDEVVEAVPEIKKTGTYMTIELPARQTIKSLDA
                     YQKKLIRNYTISSGRESMFYYESFPARSEQTYIKTKIANSTELPWLSGPAQIFLDNEF
                     MGRASVPYTPQGEDQEIVLGTYPEISSNKKLVKRYEKKGGVFGSSRVIEYNYMITVHN
                     RTSTSKNMVIMDNIPVSRNKDISVKIEDLSDEYSACENKNEETLFEQGLRKWKFVLGA
                     RAKKEISYKIIVSFDKDSEVSGL"
     gene            4912..5514
                     /locus_tag="P5123_07190"
     CDS             4912..5514
                     /locus_tag="P5123_07190"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRX16080.1"
                     /translation="MSKRKKVLIDKKFQLRTVFALLGIKIVIVSILVCVVGIYALQQN
                     KKISNIIEIEDNIVQVLSLPVATAEDFGKSPDDEALSEASEEDLAAIESSQKISLKLA
                     QDHDKNMTELRHMIKINQGLIWGIVAIIFVQGIILFFILIRQTHRIAGPVYVMTMYMK
                     SIIDGNIPEKLRPLREKDFLKDFYQVFTDMVDSLRKNKKK"
     gene            complement(5603..6307)
                     /locus_tag="P5123_07195"
     CDS             complement(5603..6307)
                     /locus_tag="P5123_07195"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRX16081.1"
                     /translation="MKKIKKVMLKSIFFPVLLFTALLYAQDFGVSDYILNPMTIDRLQ
                     FMPVTRDFRNYFILQSIDDTTIIIIGDFVGAEKKIVMIIDNESNNEIDAVHEWYPDTK
                     KLISPRKPTTNLFSDLETIKRDIISGKVFEENYAYKMRSLKSLEEKLEQGSDRFKYRH
                     GFTVKLYDPDKPNTIMSDYFFGRKDGRYDLIFKTDYYRLYHSIIRPRVVYSVYCNNSK
                     DPVVKETVENLVKYVE"
     gene            complement(6383..8794)
                     /locus_tag="P5123_07200"
     CDS             complement(6383..8794)
                     /locus_tag="P5123_07200"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRX16082.1"
                     /translation="MNIRTKKNLERLIVFMVSFALFFVLFSRIFLVKSASVGWSEKIR
                     ITQGNTEIFSYNVFSRGTLTAVAWYGSVEENRGVYASVSFNGGKSFFKPVLVGTINNL
                     DQNQIINPRVAISENGKISIVWQNFLQDDPAYRIYYSDSSDRGISWSSPDMVPLTGEM
                     QVLPLPFFDDKNSFHLVYHSLEGQQFSIYHTTKKDNEYKTPTRISLEQAQIRGAFFPA
                     VVTDKSNIYVVWQGKDNTKLLGDNLYFTYSDNYGKEWSVSRQITFGDQDDNAPQMIAH
                     DSILYLTYLNNLDSNWKVNFLKGFDYGSVWEEKSSVVYNTNIDCFAPFVYKAPNNRIG
                     ILWQINRDNRGTIYSAHYDLTELKFSEAQQLTEDNSETFKNPFAFVYNNQVSVLWNQQ
                     NAVSGVKSDIYTSPPEVFSLTHPFGKWSQFETAELKWKTVDDPSGLEGYAMIVNREPY
                     FDPTVVNLDASSNSVSAPFLTDGISYFHIRAVDKAGNYSRTIHYPLLVSNSPLSVPRI
                     RSLTHTEGESSKKRDAVFEWDMDDTVRIKGYYYSLTKDRAAEPKKFIEKTELEFKNLA
                     DGRYFLTLRGIDKADNPGRIATYELIIGESDKLTPEEYEKIALSVHQAEDNISSSDSN
                     IAPVVPISKDYAYEDLAIELRISNAVSDSERIISAEFVSNEDLVFSVKKYYYAVYKNS
                     EVINQGSSDNGIMHFKNLSPGEYSVKVWGEYVYNNDPERVVRKSDNKNVSFVIDTHDV
                     IRKEPLDIFIDQLTAIFAYNWQINSMFILTLLFSLISSIVFFRLYYVTRTAVSRIIRR
                     LILIF"
     gene            complement(8980..>9872)
                     /locus_tag="P5123_07205"
     CDS             complement(8980..>9872)
                     /locus_tag="P5123_07205"
                     /inference="COORDINATES: protein motif:HMM:NF013109.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015116 - sulfate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0008272 - sulfate transport [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="SulP family inorganic anion transporter"
                     /protein_id="HRX16083.1"
                     /translation="LLPSLSIALLGALESLLSAVVADGLIGSGHRSNIELIAQGVANL
                     ASALFGGIPATGAIARTAANIKNGGRTPIAGIVHALVLLLMLLFLAPLAGYIPLAVLA
                     GILVVVAYDMSDIQSFKMICKTNKSEISVLFVTFFLTLFFDLSVAIISSFILAVILFM
                     KRMASSLDILPIMSQKEEDSLFTAEFGEIDSRIAVFEMNGPLFFGSVAELLGVESWIG
                     SKHKVILLRMRYVPIIDSTGIMRLKALRKLIADKEAVLIISGVNSSIKKILLKNEVVG
                     KNELFDDIKAALKKANSMLK"
BASE COUNT         3074 a         2054 c         1545 g         3199 t
ORIGIN      
        1 aattaatatt ctgctccctt tgccctgcga aataaaggca agtatattca ttgaaaggac
       61 aagtcagccg tcctcaagga ttttttactg ttgaaatcct cggctgttaa agaagatctt
      121 tcatatacaa tagctataac agaaatatcc ggagtttcag agcaaacaca tatagccaga
      181 tcatcttcta cggcacagtt ccatttttca ccattcgaaa accaatactc ttctccaacc
      241 ttctcatatt ccgcgtatat tctgctatta taaaaattaa agatttctcc atccggataa
      301 tgatacgact catattgtct aaaaatacca tcagcataac tatgataatg atacacttcg
      361 ataccggctt catctacagg gtagaaaaat tccgaaccat ccttgtattc atatctctca
      421 attttccaca gccttccttc aaattcatgt tcggttccat tcatgtcccg tactgaatca
      481 tctatttcac aagacgcatt gacaaagaaa acgggtaaaa ataaaaataa tacggatagt
      541 atttttctat ttatcattca cccctccccg aaggcctttt ttattgcctc ttgtattgtt
      601 ttaacaggta ctattgaaag agatacttta agatcgttct tatcttttat attcttggca
      661 ggcacataaa ctgccgaaaa attatttctt tcaaattctg taatacgtcg ctcaaactgt
      721 gaaactgggc gaacctctcc ggccaatgaa atttcaccca aaaaaccggt tctgtcgggc
      781 aaacttcgat tactgtaacc agatgctatt gaaagggcaa ccgccagatc acttgccgtc
      841 tcgttaattg aaaatccacc ggcaaggttt ataaaaatgt catagttcga aagtttcaat
      901 tcagcatatc tctcaagtgc cgcggcaata attacaagtc tgttaaagtc cagtccatct
      961 gccgttcggc gtggattcgc aaacgaagta taagacacaa gtgactgcac ctcaaacagt
     1021 atagaacggc tgccttcaat tgccgatcca acagctacac ctggggaacc gaaacaatca
     1081 tcactaaaaa aaacgctgtt cttgtctttc acctctgaaa gaccacggtc tgtcattgta
     1141 aacaacccta tctcgttaac cgaaccgaac ctgtttttaa acgaacgtaa aatacgaaat
     1201 tctttgttaa aatcaccttc aaaatataat acagtatcaa ccacatgctc caatattttg
     1261 ggtccagcga tagagccctc ttttgtaata tgcccaatca agaaaagtgg aatattacgt
     1321 ttcttagcta ccgcagtgag cctgatagcc gaatccctca actgggaagc cgatccggct
     1381 ggtgccgaca tatttgctga cgttattgtc tgtatcgaat caataaaaac acactcaggt
     1441 ttatgctttt ctattaattc ttctattcct tcaacgctgg tacaggtggt aatcataata
     1501 ttatcttttt taatatccaa gcgatccgct ctctggcgta tctgtacagg agactcctca
     1561 ccggaaacat ataatgtttt gaacgaatta gcaatctgaa gcgaaagagt tgatttacct
     1621 attcctggct caccccctat aagcgttaca gcgcctttta cgatgcctcc accgcatacc
     1681 agatcaaatt ctctgtaacc cgatttcaaa cgaataacat catcactgtt cacttgagac
     1741 agggataccg cagtggcctt tgcctctttg gagggagcag tatccgcttc aacgattgag
     1801 ttccactccc cacattgcaa gcatttgcca gaccaagctc cgctctcggc tccacacgaa
     1861 acacaaacaa atactgtttt cttctttgcc aaactgttac ctgctggatt taaaaaatag
     1921 ttttgatggg tcctgtttta aatcataaaa aagatcccga gcatatattg aagcatctcg
     1981 aagattatta tagatatcct catcatacat cagacgacca actgttccac gtccactttc
     2041 taaacgataa acaagtttgt taagggaagc tgtggtttga gacaaatttt ttaaagttga
     2101 atcaatatcg gctttattat ctgtagagat gtcagacatg ttttttgcaa gcttgttgac
     2161 gttgaacatc agttcgcgtg tctgaacgtt caagttttca attgttgatt tggccataac
     2221 aactgatgac tttacatcat atttattatc ttcaactata tcattcatat tttcgacaaa
     2281 tttactggag tttaaaaata tatccgccag aatttctcca ccatcctgat cacgcaaaaa
     2341 actttgaaaa gtttgcatca ttctgtcaaa cgaagccgga tcaatgccat acttgatatc
     2401 gccgtcagcc aaatatccag tatcatcagg tacagccggt actattacat taatatattt
     2461 ttcgccaatc agtccggttg tataaatagc aaatgatgtt gattttgaca aacgataatt
     2521 gttatcaatc caaattttaa cttctgtttt atcctgtgac tggtttatct ctactacatg
     2581 tcctatgtct ataccgcctc cgatttttac cgaagacccc accttaagat catttaaaaa
     2641 tgtgaaatag agcctgatat tataaccgct tctgtttata ttatactgtg ccaaaagagc
     2701 cacaataaca ataaacagtg taaaactgaa agttatcatc attcccactt tggcttctgt
     2761 tgttattttc atttatacct catactccag ttattaatcc gttttcttta taggccggaa
     2821 acctctgaat ctttatcaaa actaactata attttatagc tgatctcttt tttagctctc
     2881 gctcccagaa caaatttcca tttccttaac ccctgttcaa agagggtctc ctcgttctta
     2941 ttttcgcagg cagaatattc atcggataaa tcttcaattt ttaccgatat atctttgtta
     3001 cgtgaaacag gaatattgtc cataatcacc atattctttg aagtcgaagt cctattatga
     3061 actgtaatca tgtaattata ttcaataact cttgatgatc caaacactcc gccctttttt
     3121 tcatatcgct tcacaagctt cttgttgctg cttatttcag gataagttcc aagaactatt
     3181 tcctgatctt ctccctgtgg ggtataggga actgaagcac gccccataaa ctcgttatct
     3241 aaaaaaattt gtgccggtcc agataaccag ggaagctctg tactattggc aatctttgtc
     3301 tttatatagg tctgttctga tcttgctgga aaagattcat agtaaaacat tgattctctt
     3361 cctgatgata tagtataatt tcttataagt tttttttgat aagcatccag tgattttatc
     3421 gtttgtcgag caggaagttc tatagtcata tatgtgcccg ttttttttat ttccggaact
     3481 gcctcaacaa cttcatcctt tgcggccctt tcagacattg gtatggcacg ctttacttca
     3541 tattcttcta cagcacccga ggattcctcc cttttccgct ctatatcaag aaaccaggga
     3601 taaaacactg ggacagaaat tgcaccggca ggtgaacctg tagaaatgga catcttaacg
     3661 ttttcccagt tttcaccagt ctgctgaaac agattcgcat acagagccat ttcaactttt
     3721 cccgatacgc tttgggctcg aatatcgtat tccattttcc aggcagtacc tggaatcaaa
     3781 tattttatac gtagcctagt ctttatttta cccggagaat ttatttctat aacagctctt
     3841 ttttcataat cgttttcaga aactggagtt acaagataga tgtttctttt ttcatataca
     3901 tcggtcatgt ctgcaaactt ttgaatctgt tcgcctgacg cattatcata agcagatttg
     3961 tttaaccgct ggtacaaacc gtaataattg cttcccccta tttttgaaag ttcatattcc
     4021 catttcctaa tctctttacc tagctcaatt ctcttttgtt caacactcct tttttgtaaa
     4081 tagagcgatt ttctacgtgc ggcgataaaa tcaagagtgc ttgcccaagc cttgctgttg
     4141 ggtactccac gaacagtttc atgttttgcg ttttcagccg aaacagagct aattccgtcc
     4201 agaaatttta actgcgaatt aatattgagt aatgtgtcca gatgttccct gtcccgattt
     4261 tttaataggt ctaactgttt ttccagtttt tcaatttcta gtcttcggcg agtagacaat
     4321 gcaaattttt ctatttccac tgacaagatt cgcacgtcac ttccagcaga agctttaact
     4381 gaccagtcca gaatattaac aggaagagag ccaatctcta ttcgatttaa ccctttttta
     4441 agctgaacat ccacaattct ttcaatttca cccctgttct gaaacagttt caccgaatca
     4501 attcttgata ccagtggtgc cgacataata caatcagaga aaaaaaatat taccataatt
     4561 ataccaataa ttttcataca tcccccaccg gatcttgtac taataatgcc atacataatc
     4621 agttaacatt acatgttaca aaaaatctct gctaatgcaa acaacttcat catatcaatt
     4681 gtaaatttta aaaattgtaa tcttatataa taaaatgcta cacaagctga aaaaaatcaa
     4741 cagtattatt attcttatta tgcttataat tgttaacaag cccttccttg aaagccaata
     4801 gctcaatttc gcttgttatt taatatctgt ccctcatacc tgaaaaaata ttgactttta
     4861 atcaagttta aagtaacata taattatcat tctaatcgcg gcggggttgc tatgtcaaaa
     4921 cgtaaaaagg tattaataga taagaagttt cagttgcgta cggtatttgc tcttttagga
     4981 ataaaaattg taattgtttc cattctcgtt tgtgttgtag gcatatacgc cttacagcag
     5041 aataaaaaga tctcaaatat cattgaaata gaagacaata ttgttcaggt actgtcttta
     5101 cctgttgcga cagctgaaga ttttggtaaa tcgcccgatg acgaggcgtt atctgaagca
     5161 agtgaggaag accttgccgc aatagagagt tcacaaaaaa tttctcttaa gcttgctcag
     5221 gatcacgaca agaacatgac agaattacgt cacatgataa agattaacca gggtttaatc
     5281 tggggcatcg tcgctattat ctttgttcag ggtattattc ttttctttat acttattcgc
     5341 caaactcacc gtattgcagg tcctgtttat gtaatgacca tgtacatgaa atcaattatt
     5401 gatggcaaca taccagaaaa attacgccca cttcgtgaga aagatttttt gaaagatttt
     5461 taccaggttt tcacagatat ggttgattct ttaaggaaaa acaagaaaaa atagatgatt
     5521 atatagtttg aataataaaa aaggtacttt tctaagtacc ttttttttat atatcgaaat
     5581 aaaacatatt tttttatttt tactattcta catatttaac caggttttca acagtttctt
     5641 taaccaccgg atccttcgag ttattacaat ataccgagta tactactcgc ggacggataa
     5701 tggaatgata taaccggtaa taatctgttt tgaaaatcag gtcatatcgg ccgtcctttc
     5761 ggccgaaaaa atagtcactc attattgtat ttggtttatc cggatcatac agttttactg
     5821 taaaaccgtg acgatactta aaacggtcgc taccctgttc gagtttctcc tccaaacttt
     5881 tcaaagaacg cattttatag gcataatttt cttcaaaaac cttacctgaa attatatccc
     5941 tttttattgt ctctaaatca ctaaaaagat tagttgtagg tttccttgga gatattagtt
     6001 tttttgtgtc aggataccac tcatgaacag catcaatctc gttatttgac tcattatcga
     6061 taatcattac aatttttttt tctgcaccaa caaaatcacc aataataatg attgttgtgt
     6121 catcaataga ttgcagaata aagtagttac gaaaatccct tgtcacaggc ataaactgca
     6181 accggtcaat tgtcataggg ttaagaatat aatcagaaac cccaaaatct tgtgcataca
     6241 aaagagcggt aaacaaaagc accggaaaaa aaatagactt taacatcact ttttttatct
     6301 ttttcatgtc gaactccaat ttggcattat ataccgccta tttgcaaaac atattatctt
     6361 ttcataatat atataatttc ttttaaaaga tcaagattaa tcttcggatt attcttgata
     6421 cagccgttct ggtgacataa tacaatctaa aaaatactat tgaagagata agagaaaaca
     6481 ataatgtcaa aataaacatc gaattaatct gccagttata tgcgaaaatt gcggtcaatt
     6541 gatcaataaa aatatccaat ggttcttttc tgatcacatc atgtgtatca ataacaaacg
     6601 aaacgttttt attatcactt tttctaacaa ccctttcagg atcattgtta tacacatatt
     6661 ctccccaaac ttttacgcta tattcaccag gagataaatt cttgaaatgc attattccat
     6721 tatcactact gccctgatta attacctcgc tatttttata aactgcataa tagtattttt
     6781 ttacagaaaa aacaagatct tcatttgata caaattcagc cgatattatt ctttcactat
     6841 cggaaactgc attcgaaatt cttaattcaa ttgctaaatc ttcataagca taatccttag
     6901 aaattggcac tacaggtgca atattggaat cagagctact gatattatct tctgcctgat
     6961 gaacagaaag agcaattttt tcatattctt cgggtgtaag tttatcactt tcaccaataa
     7021 tcagctcgta tgtagcaata cgtcccggat tatccgcttt atcgattcca cgaagagtta
     7081 aaaaataacg cccatctgcc aaatttttaa attcaagttc tgttttttct ataaactttt
     7141 tcggttctgc ggctctgtct tttgttaatg agtaatagta cccttttatt ctaacagtat
     7201 cgtccatatc ccattcaaat actgcatctc gttttttaga gctttcacct tctgtatgtg
     7261 taagagagcg gattcttggc actgaaaggg gactgttgct tactagaaga ggataatgta
     7321 ttgttcggct gtagttaccc gctttatcaa ctgcccgtat atgaaaatat gatataccat
     7381 cagttaaaaa aggagcgctt actgaatttg atgaagcatc aaggttaacc actgtcgggt
     7441 caaaataggg ttcccggtta acaatcatcg cgtaaccctc caagcctgaa ggatcatcaa
     7501 cagtcttcca tttcaattcg gcagtttcaa actgagacca ttttccaaaa gggtgtgtca
     7561 gtgaaaaaac ttctggagga gaggtgtaaa tatcactctt aactccggat actgcatttt
     7621 gttgattcca taataccgaa acctgattat tataaacaaa agcaaaagga ttcttaaacg
     7681 tttcagaatt atcttctgtt aactgctgtg cttctgagaa ttttagttct gttaagtcat
     7741 agtgagcaga atagatcgtg ccccgattgt ctcgatttat ttgccataga attccgattc
     7801 tattgttcgg tgccttgtaa acgaaaggag cgaaacaatc aatattggta ttgtaaacaa
     7861 ccgatgactt ttcttcccat acggatccgt agtcaaatcc ttttagaaaa ttaactttcc
     7921 agtttgaatc caagttgttc aagtaagtta gatacaatat ggaatcatga gctatcatct
     7981 gaggagcatt atcatcctga tcaccaaagg taatttgcct agacacagac cactcttttc
     8041 catagttatc tgaataggtg aaatacagat tatcacccaa gagtttggta ttgtctttac
     8101 cctgccatac aacataaata tttgacttat ccgttacaac agcggggaaa aatgctcctc
     8161 ttatttgagc ctgttcaagg gaaattcttg taggggtttt atactcatta tcctttttcg
     8221 ttgtatgata aatcgaaaac tgttgtcctt ccagtgaatg ataaacaaga tgaaaagaat
     8281 ttttatcatc aaaaaatggc aaaggaagta cctgcatctc tcccgtaagt ggaaccatat
     8341 ccgggctgct ccagctaatg ccgcgatctg aactatccga ataataaatt ctgtaagcag
     8401 gatcatcctg taagaaattt tgccatacta tggatatttt accgttttct gaaattgcta
     8461 cacgaggatt tatgatctgg ttctgatcaa gattgtttat tgtaccaacc agaacaggtt
     8521 taaaaaatga ttttccacca ttaaatgata cagaagcata tactccccta ttttcttcaa
     8581 cagagccata ccaggccaca gcggttaatg taccacgtga aaaaacatta tagctgaaaa
     8641 tttctgtatt accttgagtt atcctgattt tttccgacca acctacagat gccgatttaa
     8701 ctaaaaatat ccgtgaaaac aaaacaaaaa acaacgcaaa ggagaccata aagactataa
     8761 gtcgttccaa gttttttttt gttctaatgt tcattatttc ttatggccct tatcaagagt
     8821 aatcagtttg cacacactta gatatttcat taattatcgg taattggcgc atatattgtt
     8881 aaatttgata tcaaaaacaa tattgctatt ttaacgcaca aaaaaaggaa ccaataaaca
     8941 gttccttttt taacaagtct actactacac aaattaccct tatttaagca tactgtttgc
     9001 cttttttaaa gcagccttta tatcatcaaa caattcgttc ttaccaacta cttcattttt
     9061 taacagtatt ttttttattg agctgttcac accgctgatt attagcacag cctctttgtc
     9121 ggcaattaat ttgcgtaatg cttttaatcg cataatgcca gtactatcta ttattggaac
     9181 atagcgcatc cttaataata taactttatg tttagagcca atccagctct caacccctaa
     9241 aagttccgcg acagaaccaa agaacagagg cccattcatt tcaaatacag ctatccggct
     9301 gtcaatttcc ccgaactccg ctgtaaaaag tgaatcttct tctttttgtg acattatcgg
     9361 caaaatatcc agtgaagaag ccatacgttt catgaaaagt attactgcca aaataaaact
     9421 tgagataata gcaactgaca aatcaaaaaa cagagttaaa aagaatgtga caaaaagtac
     9481 tgaaatttcg gatttgttgg ttttacatat catcttgaaa gactgaatat cactcatgtc
     9541 atacgcgaca actacaagta ttccagctag aacggccaat gggatatatc cggctaatgg
     9601 agctaaaaag agcaacatca agagtaatac aagcgcgtgt actattcccg caatcggagt
     9661 acgccctccg ttcttaatgt ttgccgcagt tcgcgcgatt gcccctgtgg ccggtattcc
     9721 cccaaaaagg gcgcttgcca aattggccac cccttgagct atcagctcaa tatttgaacg
     9781 atgaccagat ccaatcagac catctgcgac aacagcggac agaagagact ccaaggctcc
     9841 taatagtgca atacttaatg acggtaacag ta
//
LOCUS       DAOTGZ010000088         9872 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_3064
            NODE_8161_length_9872_cov_4.671794, whole genome shotgun sequence.
ACCESSION   DAOTGZ010000088 DAOTGZ010000000
VERSION     DAOTGZ010000088.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868088
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 9872)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9872)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:22:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,785
            CDSs (total)                      :: 2,751
            Genes (coding)                    :: 2,732
            CDSs (with protein)               :: 2,732
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 12 of 19
            Pseudo Genes (internal stop)      :: 5 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9872
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8161_length_9872_cov_4.671794"
                     /isolate="AMR_MDS_3064"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1112
                     /locus_tag="P5123_07210"
     CDS             <1..1112
                     /locus_tag="P5123_07210"
                     /inference="COORDINATES: protein motif:HMM:NF012257.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="cyclic nucleotide-binding domain-containing
                     protein"
                     /protein_id="HRX16084.1"
                     /translation="KMDFQDNSFVYTSDHNNDPVKHAEMYEQKVITIDRFKELKTFPW
                     DSDIIYHESGIPPLHTPVSFLSSLPQKIQNKIHVYHIAEKDFPKDTSLNLARFGIENT
                     FYRKISASDHIEAYKILSLLKHIDFFEEIPVQKAQEFITIVKEERYQKGEKIISKGTQ
                     GDKFYIIYMGNVSVMGDDLKSRKIYGSYEYFGEVALVTRQNRAADVYAETNVILYTIT
                     RDEFLSFISGTDFERILKRLAKIRTSETWNLLSNSPHLKYCSASQKTWLESMFEPILL
                     KNSGVIQEEGKKSDFMYIIRKGSVKLCKGNETIATLSTGDIIGSMHDIHRGHTSRYTY
                     KHDSDVSLFAMKSSEISIFAEKNPGIVMKIEYNYM"
     gene            1474..3024
                     /locus_tag="P5123_07215"
     CDS             1474..3024
                     /locus_tag="P5123_07215"
                     /inference="COORDINATES: protein motif:HMM:NF023277.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3369 domain-containing protein"
                     /protein_id="HRX16085.1"
                     /translation="MDSNELMKFADDSENIMDSNLPQKFWKILIVDDEPDIHTVTKLV
                     LADSVFLGRKLEFYDCFSAKEAREFLSNNDDIAVMLLDVVMEQDDSGLKLVDYVRNVL
                     NNRTIRIVLRTGQPGQAPEEKVIVEYDINDYKAKTELTTERLYTTLYSSLRSYRDIIT
                     IDKSRRGLEKIISASSSIFEMQSLKQFVHGILMQVMSILHIDNDAVYLKTSGLTASNM
                     DGDIIILATAGKYAEQQIEKQNIDAIDDKNVRLMILEALQKKQTINRGNCYTGYFRSN
                     NGSENIIYLEHSEDINPWELDLLEIFCANIAVAFDNIYLTKEIEDSQKEMMFILGEIA
                     ESRSKETHYHVKRVGEYCKLIAILSGMDEEEAETLRVASSMHDIGKLAIPDNILFKPA
                     RFDDEEFEIMKKHSGLGSDILKFSKRSLLQKASLIAQQHHERYDGKGYPDKLKGDQIH
                     PYARITAIADVFDALSTDRVYRKAWPMEKVIHKMEEEKGRHFDPVYVDILLENLDKFL
                     DIRKKLDK"
     gene            3117..3338
                     /locus_tag="P5123_07220"
     CDS             3117..3338
                     /locus_tag="P5123_07220"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRX16086.1"
                     /translation="MMTQRIGEQNFQNSALFINNANEKHNERSEVNILKSKEINQLAA
                     KFENSGIFTAEASKILAQNILNGSVDVYV"
     gene            3416..4246
                     /gene="rsmA"
                     /locus_tag="P5123_07225"
     CDS             3416..4246
                     /gene="rsmA"
                     /locus_tag="P5123_07225"
                     /EC_number="2.1.1.182"
                     /inference="COORDINATES: protein motif:HMM:TIGR00755.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000179 - rRNA
                     (adenine-N6,N6-)-dimethyltransferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (adenine(1518)-N(6)/adenine(1519)-N(6))-
                     dimethyltransferase RsmA"
                     /protein_id="HRX16087.1"
                     /translation="MNKQKITSILEDYGLRPSKKFGQNFLINDEISQKILSSSLLTEG
                     SRILEIGPGLGCMTQFLVETGLPVIAVEIDKGYVNVLKDTFYTANNFTLIHDDFLRFQ
                     PDFTFTHLIANLPYYCASELIFKVAEFNPEVITVMLQKEMAERIKAKPGSKSYGLLTV
                     NLTIKYEFHEMFTVGKSNFYPRPDIDSAVIVLKRRENNLIEENLIPLFKQITRSLFWG
                     RRKTIAKCLTSSPHIAAKREDVLKVIEASALDSNLRAEDLSLTDFVKLTKEFMRLNLA
                     "
     gene            4243..6669
                     /locus_tag="P5123_07230"
     CDS             4243..6669
                     /locus_tag="P5123_07230"
                     /inference="COORDINATES: protein motif:HMM:TIGR03960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR03960 family B12-binding radical SAM
                     protein"
                     /protein_id="HRX16088.1"
                     /translation="MRYTFEQIVPILNRVEKPGRYTGGEKNSVQPEDGDFRFALGYPD
                     LYEIGMANQGIQILYSRLNETKGLSCERFYAPGKDLFECLKQSGLNLFTLETFTPLNE
                     MEAVGFNLSHELLYTNMLYILESAGIAIFSRERLESDPIIIAGGEAVSNPAPVSLFVD
                     CFFAGEGEEGIIEIAESLKINRNLNRLEKLKALSLIEGVMVPSLYKTAINGDGMVIFE
                     GPRVNKRNISGQSLRNPLKPVIPSVRTSQERAVIEITRGCDNLCKFCHAGYYTLPLRQ
                     CSVEKTAQETFEVLENTGYNGVTFTSLSISDYAQLIPLLNRVLPDLNRKGVNVNLPSM
                     KVDKGTLPIITTISGVKQVNLTFAVEAAHTEIRKLIHKRVQVDELLEIVRYVFENGWK
                     TIKLYFMLGLPGYKEYDEAQSIIDLLKQIFDIGRGKKSINATVSPFIPKPHTPLDMND
                     MATAEYFNSVVDKLKKALPRKIAVKNHNIDSSIVEGFLARGDIECGRALHEAYTAGAV
                     FDSWSEHFNSNAWLPVVEKYMHRADYFCERTDENQSWSVILTGAEKLVSAKRDTVLSE
                     EDLGRKRMRHEVPFDYESINRSMEDFEKSYEKLLSVRIVFSKKGRARYISHLDLVEII
                     KRAFNIAKLPICYSRGFNKRAHIGGGFPLPLGIDSECEVFDCDLYSEMPVGVEDVIGS
                     NLPEGVELVKIYPHNGDTIALHKNSVSSYRICGNSNIIEKIEKAIDSDITLTKKTKKG
                     VTEIKAADSVHSVQRGDNNITLFLYTGSTNALRIDALMRAFVNDDEYNSLVITKIAIY
                     NDENGLTNLL"
     gene            6793..6942
                     /locus_tag="P5123_07235"
     CDS             6793..6942
                     /locus_tag="P5123_07235"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRX16089.1"
                     /translation="MEIQNISGSDIVQTTMNRTEEPAQETESIVQQRENVQEENKGNT
                     IDNYA"
     gene            6955..7773
                     /locus_tag="P5123_07240"
     CDS             6955..7773
                     /locus_tag="P5123_07240"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRX16090.1"
                     /translation="MAATKEQKQQYDEAIREPRARMKELEKELKDLLAKKKKYPAIGG
                     YYNLEMVLLHLEIIMLNINMNDSSIEYLDLRNSAFLERGRKEFYKALQLLEEIAGNEL
                     EKPLSENKEYLKSIDRVNIRNILQIVRKFSWVFETLKDRVGSTSKWKWAFVDIYVRVA
                     AVIKNMINFSELEQHRNFRSEFFKDREELLKFCKANLEEAAKESRNKYEMSTKAPEDI
                     LKAIELLSSLHNIHVLYGEVNEAQKTKTVIDALRARIDTEEKKNTKAKAKAAKV"
     gene            7846..8670
                     /locus_tag="P5123_07245"
     CDS             7846..8670
                     /locus_tag="P5123_07245"
                     /inference="COORDINATES: protein motif:HMM:NF023568.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HRX16091.1"
                     /translation="MKINRINPDCATVQNGLPLYLQGKNDKAVLLIHGYNGYVHDVSF
                     LAHKLNKAGFTVSVPRNTGCGTDHTDFLCTKSRDWIRHAVDCYLDLKAVYKDVYLVGL
                     SMGALISIILASHFRVKKLVLAAPAVILISPVLPFTSFLRIFIKKIKRNTKFKPLNPV
                     NNRTFAKEWKFLNSEYWSWHYVPALYQLQKIRRQAIKAIKKVKSDTLVLASKSDLTVS
                     LKTVIYLRDKCSAEKFESRIFEKSSHVITDSVEREDVAKAVIDWLIDKPTRRDRQK"
     gene            8707..9276
                     /locus_tag="P5123_07250"
     CDS             8707..9276
                     /locus_tag="P5123_07250"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRX16092.1"
                     /translation="MEHSSYQITGDTTNIEAIMKNELLSVSRTYVKTNETKICSSESA
                     LVVDLGTCVSVVLSGITSEGRLYFGANHLFKSRDENSDKSLEQISSLYNELHDKKVTR
                     IQCIGVFGAGYREKSLASQVAQNNIITVLETLSLYKLDIEIFQTGFSQGLLLYHSQSL
                     DSIVVRLKNLGDRTFTYYKLPIKKIFNKW"
     gene            9302..>9872
                     /locus_tag="P5123_07255"
     CDS             9302..>9872
                     /locus_tag="P5123_07255"
                     /inference="COORDINATES: protein motif:HMM:NF024874.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HRX16093.1"
                     /translation="MSFIKISVLMVGVSLILLVINMIIKANEAENKFKYNPELEFIID
                     YPGNPVDKNGRFVNEPPTTLSGFTDIIKWKFSDNPQEEEKKNENYSLPVKKGCDFLEE
                     EKDVIVWLGHASFFIRLNGVSMLIDPVFDKATVVPRLAGNACDISNYKNIDYLLISHN
                     HRDHLDIPTIAELYKNNPYIHALIPLEMGK"
BASE COUNT         3318 a         1683 c         2023 g         2848 t
ORIGIN      
        1 ttaaaatgga ttttcaagat aacagttttg tctatacttc ggaccataac aatgatccgg
       61 tcaagcatgc cgagatgtat gagcaaaaag ttattactat agaccgcttc aaagaattga
      121 aaacatttcc atgggattcg gatataattt atcatgaatc tggaatacct ccgcttcata
      181 ctcccgtatc ttttttatca tcactgcccc aaaaaattca aaataagatt cacgtatacc
      241 acatcgccga aaaggatttt cctaaagata ccagtctcaa tctggcaaga tttggtatcg
      301 aaaatacttt ttatcgcaaa ataagcgcct ctgatcatat tgaggcctat aagatattga
      361 gccttcttaa gcatatagat ttttttgaag agatacctgt tcaaaaagca caggagttta
      421 taaccattgt aaaagaggag cgttaccaga aaggtgagaa gatcatctct aagggaacac
      481 agggggacaa gttttacata atttatatgg gtaatgtttc tgtaatgggt gatgatctta
      541 aaagccgtaa gatttatggg tcttacgagt actttggcga ggtagcgttg gttactaggc
      601 aaaaccgtgc tgcggatgta tatgccgaaa ccaatgttat actttatact ataacacgtg
      661 atgagttttt aagctttata tcaggaacag attttgagag aatactaaaa agactagcca
      721 agataagaac aagtgagaca tggaatcttt tatcaaacag cccgcattta aaatactgtt
      781 cggcatctca aaagacatgg cttgaatcga tgtttgaacc aattttgtta aaaaatagcg
      841 gggttataca agaagaaggg aaaaagtccg actttatgta tattatacga aaaggctcgg
      901 tgaaattgtg taagggtaac gaaacaattg ctactctttc gacaggcgat attatcggtt
      961 cgatgcatga tattcacagg ggacatacat cacgttatac atataaacat gattcagatg
     1021 tctctctatt tgcgatgaaa agcagtgaaa tttcaatttt tgctgaaaag aatccaggca
     1081 ttgttatgaa aattgaatac aactatatgt gattattatt gttctgatca ttttcctatt
     1141 tattattagg aacctctata gattaatttt tgtacaatga acttgcggca aattccaact
     1201 acaaaacaaa aaatattact agccccataa gtaatatttt tggacttttt tcgattggca
     1261 acaataaaaa aattctttaa attaaataat agtgaaagcc attggaaaaa gaaatttccg
     1321 aagttcccta atcaaattaa ttaataaaca ttctttgttg ctaaattaca atatttatgg
     1381 taaaatagac tatactaatc ggtcaattga atcatcgcga atcataaaaa gttgtttttt
     1441 gttttttata ctatggaatt tttaggggga tttatggaca gtaacgagtt aatgaagttt
     1501 gcggatgact ctgaaaacat aatggattcg aatcttccac aaaaattctg gaaaatactg
     1561 atagtcgatg acgaaccgga tatacatacc gtaacaaaac ttgttcttgc cgattcggtt
     1621 tttctgggac gcaaacttga attttatgac tgtttttcgg caaaagaggc aagagaattt
     1681 ttaagcaata atgatgatat tgctgttatg ttgctcgatg tagtaatgga acaggatgat
     1741 tctgggttaa aacttgttga ttacgtacgt aatgttctta ataaccgaac aatacgtata
     1801 gttttacgta ccgggcaacc gggccaggct ccagaagaga aggttatcgt tgagtacgac
     1861 attaacgatt ataaggcaaa aacagaactc actacagaga gattatatac tactttatac
     1921 tcttcccttc gatcataccg agatataatc acaatagata aaagccgtag aggtcttgaa
     1981 aaaataattt ctgcctcttc aagtatcttc gagatgcaat ctttaaaaca gtttgttcac
     2041 ggaatactta tgcaggttat gtcgatactg catattgaca atgacgctgt atatttaaaa
     2101 acttcgggtt taactgcatc aaatatggat ggtgacatta ttattctggc cacagcggga
     2161 aagtacgcag agcaacagat agagaaacag aatattgatg cgatagatga caagaatgtc
     2221 aggcttatga ttcttgaagc tttgcaaaaa aaacagacaa taaacagagg taactgttat
     2281 actggatact tccgaagtaa taatggatct gaaaacataa tatatcttga gcattcagaa
     2341 gatataaatc catgggagct tgacctgctt gaaatatttt gtgctaatat tgctgtggca
     2401 tttgataata tttatctgac aaaggagatt gaggattcac aaaaagagat gatgtttatc
     2461 cttggtgaaa tagctgaaag ccgctctaaa gagacccatt atcatgtaaa acgggtgggc
     2521 gaatattgta aactgatagc cattctaagt ggaatggatg aggaagaagc cgaaacatta
     2581 cgggttgcat cttccatgca cgatattggg aaacttgcca tacctgataa tattctattt
     2641 aaacctgcac gttttgatga tgaagagttc gagatcatga aaaaacattc aggacttggt
     2701 tctgacatac taaagttcag taagcgatct ttgttgcaaa aagcgtcgtt aatcgcgcaa
     2761 caacatcatg agcgctatga cggtaaaggt tatcctgaca aattaaaagg cgatcagata
     2821 catccatacg cccgcataac ggcgatagcc gatgtgtttg acgctctatc taccgacagg
     2881 gtttatcgta aagcctggcc tatggaaaag gtaatacata aaatggaaga ggagaagggg
     2941 cgacactttg atccagttta tgtagatata ttgcttgaaa atctggataa atttcttgat
     3001 attcgcaaaa aactggataa atagaatttt ttactaaagc ttcaccctgt tttttcgatt
     3061 atatgagtaa gaggttttta aacctttacc gtatgataaa acagggaggt tttattatga
     3121 tgactcaacg gattggggaa cagaattttc aaaacagcgc gctttttatc aataatgcca
     3181 atgaaaaaca taatgagcgc tcggaagtca atattctaaa aagcaaagag atcaatcaat
     3241 tggctgcgaa attcgaaaat agcggtattt tcactgccga ggcttctaaa attcttgctc
     3301 agaatatctt gaacggctct gttgacgttt acgtctaaaa ccattgcttt agagccgatc
     3361 ttcttcaata atatcttatt gacaaagctc atgccttaaa acccttattc ttttcatgaa
     3421 caaacaaaaa atcacctcta tacttgaaga ttatggattg cgtccctcta aaaaatttgg
     3481 gcaaaatttc ttaataaatg atgagataag ccaaaaaata ttatcttcat ctctattgac
     3541 tgaggggtca cgaatactcg agataggtcc tggccttggc tgcatgacac agtttctagt
     3601 cgaaaccgga ttaccagtta tagcagtgga aatagataaa ggctatgtta atgtattgaa
     3661 ggacacgttt tacacagcaa acaacttcac ccttatacat gatgattttt taagatttca
     3721 acctgatttt acttttaccc atcttatagc gaacttgcca tactattgcg cttctgaact
     3781 tattttcaaa gtcgcggaat tcaatcctga agttattacg gtaatgcttc aaaaagagat
     3841 ggccgagaga atcaaggcaa aacctggcag caaaagttat ggccttttga cagttaatct
     3901 tacaattaaa tatgagtttc acgaaatgtt cacagtcgga aaatcgaact tttatccaag
     3961 gccggatatc gattctgctg ttatagtatt aaaaagacgc gaaaacaatt taatagaaga
     4021 aaatcttata cctctattta agcagatcac tcgttcactt ttttggggac gccgtaaaac
     4081 catagctaaa tgtttgacca gctctccaca tattgccgca aaaagagaag atgttttaaa
     4141 agtgattgag gcatcagctc ttgattctaa tcttagggca gaagatttat cacttacaga
     4201 ttttgttaaa cttacaaaag aatttatgag gctaaacctg gcatgagata tacctttgaa
     4261 cagatagtac ccatactaaa cagagtagaa aaaccgggaa gatataccgg tggagaaaaa
     4321 aacagcgtac agcctgaaga cggtgatttc agatttgcac ttgggtatcc cgatctgtac
     4381 gagatcggga tggcaaacca aggcatacaa atcttgtata gtaggctaaa cgaaacaaaa
     4441 gggctttctt gtgaaagatt ttatgcgcca ggaaaagacc tgtttgaatg tttaaaacag
     4501 tcagggctaa atcttttcac tcttgagaca tttacgccct tgaacgagat ggaagcagtt
     4561 ggttttaatt tgtcacacga acttttgtat acaaatatgc tatacatact tgaaagcgct
     4621 ggtatcgcaa ttttttcaag agaaaggcta gaaagtgacc ctataataat cgcaggtggt
     4681 gaggctgtat cgaatcctgc tccggtatca cttttcgttg actgtttttt tgcaggtgaa
     4741 ggtgaagagg gaataatcga aattgccgag tcgcttaaaa ttaaccgtaa cttgaaccga
     4801 ttggagaagc taaaagccct ttcgcttata gagggggtaa tggttccatc actgtacaaa
     4861 accgctatta acggtgacgg catggtgatt ttcgaaggac cgagagttaa taaaagaaat
     4921 atatcgggac aatcattaag aaatccgcta aaaccagtta taccttcggt gcgtacttca
     4981 caggagcgtg cggtaatcga aataactcgt ggctgtgata atctatgtaa attctgtcat
     5041 gccggatatt atacattgcc attacgccaa tgtagtgtag agaaaacggc tcaggaaacc
     5101 tttgaggtac tagaaaatac aggctataac ggtgttacat ttacctcact ttcgataagc
     5161 gactatgccc agcttatacc gcttctaaac agggtgcttc ctgatcttaa ccgaaagggt
     5221 gtaaacgtaa acctgccttc aatgaaagta gacaaaggta ctttgccaat aataacaaca
     5281 atatctggag taaagcaggt taacctgact tttgccgttg aagcagcaca taccgagata
     5341 cgtaagttga tacataaacg agtgcaagta gatgaacttc ttgaaatagt acgctatgtg
     5401 tttgaaaacg gctggaaaac tattaagctg tacttcatgt taggtcttcc cggctataaa
     5461 gagtacgacg aagcgcaatc aataattgat cttttaaaac agatctttga tataggacga
     5521 gggaaaaaaa gcattaacgc gacggtatct ccttttattc ccaagcctca tacacctctt
     5581 gatatgaatg atatggcaac agcagaatat tttaacagcg ttgtagataa acttaaaaaa
     5641 gctctaccgc gcaaaatagc tgtaaaaaat cataatattg attcgtccat tgtagaaggc
     5701 tttttggcac gaggtgacat tgagtgcgga cgggctttgc atgaagcata cacggctgga
     5761 gcagtgtttg attcatggag tgagcacttt aacagtaatg catggctacc tgttgtagag
     5821 aaatacatgc acagggccga ctatttttgt gagcgtactg acgagaatca gagttggtct
     5881 gttattttga ctggagccga aaagctggtt tctgcgaagc gtgatactgt gctaagcgaa
     5941 gaagatctgg ggcgcaaaag aatgcggcac gaggtaccgt ttgactatga atctattaat
     6001 agatcaatgg aagattttga gaaaagctat gaaaaacttt taagcgtgcg tatagtattt
     6061 agtaaaaagg ggcgggcacg atatatatca catctggatc ttgtagagat aattaaacgc
     6121 gcatttaata tagcaaagtt gcccatttgt tacagtcgag gatttaacaa acgcgcgcat
     6181 ataggtggtg gttttcccct gccgcttggt atcgatagtg aatgtgaagt gtttgactgt
     6241 gacctctatt cagaaatgcc ggttggagtc gaagatgtaa ttggttcaaa tttacccgaa
     6301 ggggtagagc ttgtaaagat atatccgcat aatggtgata cgatcgcgtt acataaaaac
     6361 agtgtgagct cttatagaat ttgtggaaac agcaatatca ttgaaaagat agagaaggct
     6421 atcgattcgg acataacctt gaccaagaag acaaaaaaag gtgtaacaga aattaaagca
     6481 gctgattcag ttcacagtgt acagaggggt gacaacaata taacactttt tctttataca
     6541 ggcagcacaa atgctttacg cattgatgcg cttatgcggg cattcgtaaa tgatgatgag
     6601 tataatagcc tggtaattac aaaaattgct atttataacg atgaaaatgg attgaccaac
     6661 cttttatgac aacaaaacgg gcctctcttt gatattctta ttaagaataa taaagataat
     6721 aaattttaga tgttttattg ttgacctttg tgtatattta agtgagaata tcaatgcgta
     6781 cggaggtaca aaatggaaat acagaatatc tcagggtcgg atatagtaca gacaaccatg
     6841 aatagaacag aagaaccggc gcaagaaact gaatccattg ttcagcaaag ggaaaatgtg
     6901 caagaagaga acaaaggaaa tacaatagat aattacgcgt aagccggagc taagatggca
     6961 gctaccaaag agcaaaagca gcagtatgat gaggccataa gagagcctag agcccgtatg
     7021 aaagagcttg aaaaagagct taaggattta ctggcaaaaa aaaagaaata tccagctatt
     7081 ggcggttatt ataatcttga gatggtgcta cttcatcttg agattataat gctaaatata
     7141 aatatgaatg attcatcaat tgagtatctt gatctgcgta actctgcttt tcttgagcgc
     7201 ggtcgtaagg aattttataa ggctctacaa ctacttgaag aaattgccgg aaacgagctc
     7261 gagaaacctc tttctgaaaa caaggagtac ctaaaatcca ttgaccgagt taatatccga
     7321 aatatacttc agatagtacg taagttttca tgggtgtttg agacgcttaa agaccgtgtg
     7381 ggctctacat ccaaatggaa atgggcattt gtggatatat atgtacgtgt tgctgccgta
     7441 ataaaaaaca tgattaattt ctctgaactt gaacagcatc gcaattttag atccgaattt
     7501 tttaaagacc gtgaagaact gcttaagttt tgcaaggcta atctggaaga ggcggcaaaa
     7561 gagtcacgaa ataagtacga gatgtctacc aaggcacccg aagatatact aaaagcaatt
     7621 gaacttttgt cctcattaca taatattcat gtattatacg gcgaagttaa cgaggctcag
     7681 aaaaccaaga ccgtaataga tgcccttcgt gcccgtattg atacagaaga gaaaaagaat
     7741 accaaggcca aagctaaggc cgcaaaggta taaatacctg ttcgatttat ccttttttat
     7801 gtacttagca aataatcaaa aagcctgact gatttcggaa gaactatgaa gattaacaga
     7861 attaaccctg attgtgcaac agtgcaaaac ggacttccct tatatttgca aggcaaaaat
     7921 gacaaagctg ttttattgat tcatggatac aacggttatg ttcatgatgt aagttttttg
     7981 gcacataaat tgaataaggc aggctttacg gtctctgttc cacgtaatac tggatgcgga
     8041 accgaccata ccgatttttt atgtacaaag tcacgtgact ggattcggca cgcggtcgat
     8101 tgctaccttg acctgaaagc tgtgtataaa gatgtctatc tggttggtct ttcaatgggg
     8161 gctctaatct caattatttt ggcttcacat tttagagtca agaagcttgt gcttgctgca
     8221 cccgcggtaa tactgattag cccggttctg ccgtttacct cttttttaag aatttttatc
     8281 aaaaagatta aaagaaatac caagttcaag cctcttaatc ctgttaacaa tcgaactttt
     8341 gctaaagagt ggaagttttt aaacagtgaa tattggagct ggcactacgt tcctgctctt
     8401 tatcagctgc aaaagatacg tcgtcaggcc ataaaggcca taaaaaaggt aaaatctgat
     8461 actcttgtgc ttgcctcaaa aagcgattta acggtttcat taaaaacagt catctatttg
     8521 cgtgacaaat gctctgccga aaagtttgaa tcaaggattt ttgaaaagag ctcacatgtg
     8581 ataaccgaca gtgtcgagcg tgaagatgtc gccaaagctg taatcgactg gttaattgat
     8641 aagccgacca gacgagacag acaaaaatag agttataaaa tgatacggga tttgaactga
     8701 ttatatatgg agcattcaag ttatcagata actggtgata caacgaatat agaggctata
     8761 atgaaaaacg agcttctgtc tgtatcgcgt acctatgtta aaaccaacga aacaaaaata
     8821 tgctccagtg agtcggcact ggtggttgat cttggaacat gcgtaagcgt agttttgagc
     8881 ggaatcactt ccgagggcag gctctatttc ggagccaacc acctgtttaa aagcagagac
     8941 gaaaacagtg acaagtctct tgagcagatt agctcgcttt ataatgaact tcacgacaag
     9001 aaggtaacac ggatacagtg tattggtgtt tttggtgccg gttaccgtga aaagagtctg
     9061 gcttcgcagg tagcacagaa taatattata accgtattag aaaccttgag cctttataaa
     9121 ctggatatag aaatattcca gacaggtttc agccaagggt tactgcttta tcattcacaa
     9181 tccctggatt caattgttgt taggctaaaa aatctgggcg atagaacttt tacctactac
     9241 aaactgccta taaaaaaaat atttaataaa tggtaatttt taaagcttct ttgaggtaaa
     9301 tatgagtttt ataaaaatat ctgtgcttat ggtgggagta tctcttattt tactggttat
     9361 aaatatgatt attaaggcaa acgaagctga aaataaattt aagtacaatc cagagctgga
     9421 atttattatt gattatccgg gaaaccctgt ggataaaaac ggacgttttg taaatgagcc
     9481 gccaactacc ttgagcggat ttacagatat tattaagtgg aaattttctg acaatccgca
     9541 agaagaagag aaaaaaaacg agaattacag tttacccgta aaaaagggat gcgacttttt
     9601 agaagaagag aaagatgtga ttgtctggtt gggccacgcc tcatttttta tacggcttaa
     9661 cggggtatcg atgctgattg atcctgtatt tgacaaggca acagttgttc cccgacttgc
     9721 cggcaatgca tgtgatatat caaactataa aaacattgat tatctgctta tatctcataa
     9781 ccaccgagat catttggata ttccgactat tgcagaactg tataagaata atccctatat
     9841 acatgccctt ataccccttg aaatggggaa ag
//
LOCUS       DAOTGZ010000089         9853 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_3064
            NODE_8178_length_9853_cov_5.059604, whole genome shotgun sequence.
ACCESSION   DAOTGZ010000089 DAOTGZ010000000
VERSION     DAOTGZ010000089.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868088
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 9853)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9853)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:22:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,785
            CDSs (total)                      :: 2,751
            Genes (coding)                    :: 2,732
            CDSs (with protein)               :: 2,732
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 12 of 19
            Pseudo Genes (internal stop)      :: 5 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9853
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8178_length_9853_cov_5.059604"
                     /isolate="AMR_MDS_3064"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            213..947
                     /locus_tag="P5123_07260"
     CDS             213..947
                     /locus_tag="P5123_07260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020267779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fic family protein"
                     /protein_id="HRX16094.1"
                     /translation="MKEILDKIDKLHSRINQMRPLEKETLASLRNYYKIGLTWSSNAL
                     EGNTLTESETKVVIEDGLTIGGKPLKDHFEASGHADAFDEVIRLSQKADITEIDILKL
                     HRLFFLKIDDKNAGAYRTKTVFISGSHYPLPSPERVPALMKEFTDLVSCSRKTEHPVI
                     AAAKAHLQFVFIHPFVDGNGRVARLLMNLILLQQGYQIAIVPPVLRGEYIASIEAAHK
                     DETDFIKLICRSVYETQKDYLRMLNG"
     assembly_gap    1368..1467
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<1468..2195)
                     /locus_tag="P5123_07265"
     CDS             complement(<1468..2195)
                     /locus_tag="P5123_07265"
                     /inference="COORDINATES: protein motif:HMM:NF016629.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rpn family recombination-promoting
                     nuclease/putative transposase"
                     /protein_id="HRX16095.1"
                     /translation="MTKPHDNAYKFLFSNKRIFLQLLKSFVHEDFVKGLTENNLELAD
                     KTFVSEEMLDRESDLLYKINLNKNETYIYILMEFQSTPDKAIPIRMFQYILLLYDQLL
                     KNSKKGLLPNVFPLLLYNGSKKWTVPRNIGDLIEKNIPANYIPSFEYFAIIQNEISDT
                     FIDKLSNLIAAVIKIENAGNEKKLRVAIDRIVMYIKDEDPSDIRDYFLWLKNMFKVEL
                     KEIELNSIRNLMGVKPMLAELVEK"
     gene            2334..2774
                     /locus_tag="P5123_07270"
     CDS             2334..2774
                     /locus_tag="P5123_07270"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRX16096.1"
                     /translation="MNTNNSFMKQWFDGVEDFLESSGEVARKDFLSCCAKRCSDSYPL
                     ALYKEAFSDGRSLPEALKYLANNFDGFDYAVFSDRVELSYSQCGCDLYKEGLITSVNL
                     CQCSEASLLYIWEQIYGKGAVAVTTKKTIIGGNDSCLFEIRILK"
     gene            3085..4980
                     /locus_tag="P5123_07275"
     CDS             3085..4980
                     /locus_tag="P5123_07275"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HRX16097.1"
                     /translation="MNLINANAVFYSNGSKTILRDASFCVQKSKKYALIGPNGTGKTT
                     LLRLITGELEVESGTITCKESLKWSLVPQEAEPGDGRTIREYMLSPLAPVIKKMAMLE
                     KQMQTARDEELADLLVEYQKVQDLFEKTGGYGAEDNALNLLASLGVQNDPDQIVDTLS
                     GGEQSLLFFARALAGDPELLILDEPGNHLDYLGLAWLESFLQNFRGAVIIVSHNRYMI
                     NRICDEIWHLQNGSLTVFNGNYNRFKTSYLRDGAIRQNEYQKAQKEIGELNTRISRLQ
                     SIARSQYNPPPGVLSELKSLMAKKQRIEQNMPDKQDILADTVKISVNAQAVQADIACE
                     VKNLCLSAGDTFLADNVSFTLHCGERAAIEGLNGTGKTTLLEKIVKEGSRDSDIIRIG
                     PSQKPGYLSQKPQFATDAQTIADEVKSWGQLSRDEVYDTIKKFLFTYTDLDKKLSVLS
                     GGEKNRLQLARLIYCKANFLVLDEPTNHMDIQTREVMDEVLTGFAGTILFVSHDRYFL
                     DAVAQRVIEIADKKITVHQGNFTDFFKKRYRTLPRLSGSVTTRAKKRNPYNDSASKSA
                     SGQKEKQLENRIEEMEQEIACLTHELELCVSQKNSAKGRQLASRLDKLDNALTRLYAE
                     WDSVCKI"
     gene            5080..5220
                     /locus_tag="P5123_07280"
     CDS             5080..5220
                     /locus_tag="P5123_07280"
                     /inference="COORDINATES: protein motif:HMM:NF021253.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="addiction module protein"
                     /protein_id="HRX16098.1"
                     /translation="MADLDKPDPEIDRIWAEEARNRWAAHKAGRIPAVSYSDVMGKYR
                     RI"
     gene            complement(5291..5689)
                     /locus_tag="P5123_07285"
     CDS             complement(5291..5689)
                     /locus_tag="P5123_07285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014276320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system VapC family
                     toxin"
                     /protein_id="HRX16099.1"
                     /translation="MTYLLDTNVCIKYLNGQSDEIVKKISSFSPDDIVICSVVRAELF
                     AGDYKSKSFKKTYDKLINFLNVFPSIPFDDEASDIFGDIRAQLEKKGKPIGPFDLQIA
                     SIALLNDLILVTHNTKEFSRIIKLEIEDWE"
     gene            complement(5686..5919)
                     /locus_tag="P5123_07290"
     CDS             complement(5686..5919)
                     /locus_tag="P5123_07290"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRX16100.1"
                     /translation="MKTINLRSRIGRDGILNLKVPTTEKEVDVEVVIILQPKNNSKRL
                     WPENFFENTYGCLRNDQIERLPQGDFPQREQLL"
     gene            6297..6536
                     /locus_tag="P5123_07295"
     CDS             6297..6536
                     /locus_tag="P5123_07295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020610880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0110001 - toxin-antitoxin complex
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system Phd/YefM family
                     antitoxin"
                     /protein_id="HRX16101.1"
                     /translation="MKTLPVGEFKTHFSEILDCIKNGEEIAFSYGKGKEKIAVLVPYA
                     KYSKKRKRNLGLLQNKASFEIQSDFKITDDEFLVL"
     gene            complement(6839..7213)
                     /locus_tag="P5123_07300"
     CDS             complement(6839..7213)
                     /locus_tag="P5123_07300"
                     /inference="COORDINATES: protein motif:HMM:NF016370.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF559 domain-containing protein"
                     /protein_id="HRX16102.1"
                     /translation="MLYNETKVNTPKYVIEFARQNRQNLTVSEQKIWSQLRNKQLNGY
                     KFRCQHPISRYILDFYCHKAYLAIEIDGDIHKKRVDYDNYRDKFLESIGITTLRFSVE
                     EVMSNNCTVINKILTSLLKKDE"
     gene            complement(7385..7918)
                     /locus_tag="P5123_07305"
     CDS             complement(7385..7918)
                     /locus_tag="P5123_07305"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRX16103.1"
                     /translation="MKLKEYKNLSNFLNVLFDFFKENNLTGNFYYENDTDKLKNILLE
                     SQFVSYNLSPFIDQYSIDFKTDNNANNANIANQSFVEYIIFGSEHDNKSTGLLIDVSE
                     KGSSCNSFFQLSWYEELFPPFVYDFFDSDGMLTINHYEKESKVKIEPKEIMSFDDFEK
                     HLKKNRFKKNLNVIDVE"
     gene            8042..8230
                     /locus_tag="P5123_07310"
     CDS             8042..8230
                     /locus_tag="P5123_07310"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRX16104.1"
                     /translation="MLFVKNYSLPHGFAYSNNDSREAAKARRKDEGAREGAKARRKDK
                     EGSREGDRARRKDEEVLR"
     gene            complement(8448..9344)
                     /locus_tag="P5123_07315"
     CDS             complement(8448..9344)
                     /locus_tag="P5123_07315"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRX16105.1"
                     /translation="MKNPIKLTVTISVFIIAFLFLFSCDDSDDSSTDEPFTHDPIEED
                     FDEADKPSYETKTYDDNGTSQSYELMLPWNYDKDHNSNRSYPLMINVHYSGSLVAGDT
                     QRMQDYPCFCLNAASNNDWTYAMIAQLVDDYRIDTDRIYLAGFSAGGSGSYPFASNLE
                     AKQRLTVAGIVRCAGGSNPELSEAIADKTSVWYHVGLSDSYDFGYGNGLLSEDPDDER
                     RISNQAYAYVKGLDSSSKATESVETKTVSGYECTTTSLSLAGVEIFKRSHYAGMGHSS
                     PAYSDPEVIKWLFNQHLSNRVE"
BASE COUNT         2994 a         1662 c         2041 g         3056 t
ORIGIN      
        1 taatcgagca gattattgaa atataattac cactcttctg ctaacataaa aataatcgaa
       61 aagttgcttg cgtgctgttt gtaataaggt aaagtgagtg agtaaaaagc catttagttg
      121 tgaatatggt cgatacttta taatcgtttg tgcagagaaa atggaagcga aaatagaagt
      181 gaagatgaat gcggaattgg tggatgcgta ttatgaaaga gatattggat aaaatcgata
      241 aactgcattc ccggataaac caaatgcgtc cgcttgaaaa ggagacactc gcatctttac
      301 gaaattatta taagattgga ttaacctggt ccagtaatgc tctggagggg aatactctta
      361 cggaatcgga aacaaaggtg gttatagaag acgggcttac aatagggggt aagcctttaa
      421 aagatcattt tgaagcaagt ggtcatgccg atgcctttga tgaagtaatc agactctctc
      481 aaaaagctga cataaccgag atagatatat taaaactgca tcgtctattt tttttaaaaa
      541 tagatgataa aaatgccgga gcataccgta caaaaacggt ttttatatcg ggttcgcact
      601 atcctttgcc ctcgccagaa agggttcctg cattaatgaa agaatttaca gatcttgttt
      661 cttgttccag aaaaacggaa catccggtta ttgcagccgc aaaggctcat cttcaatttg
      721 tatttattca tccctttgtt gatggtaacg gcagggtagc aaggttgtta atgaatctga
      781 tacttcttca gcagggttat cagatagcga tagtacctcc tgttttgcgc ggcgaatata
      841 tagcttcgat cgaagccgcg cataaagatg aaacagactt tataaagctg atctgtagat
      901 ctgtttatga aacacaaaaa gattatttgc gcatgctaaa cggataattg atagtacaaa
      961 actgagtaca ggtgtgctat aaaaaatata tttaatgtaa aattgctttt tcaaagagga
     1021 tacattacga taacatttct tttttctgat ataaaaccag agactgcagc atatatgagc
     1081 ttaaaggtaa aacgaataaa attactgttt attctgtatt accggattaa cagatccata
     1141 ttgtaactta atagagagga aaattgtgaa aagaataaca cttgcacgta taattacctt
     1201 tgtagtgatt tttattttga tgtttatgtt tcaacgaagg tttttaattt tttcaacagg
     1261 aagttcagtt aattctgata tctcctctat ggaaaatccc ttagctaaca ttttagaagc
     1321 tgtttgaagt ttgccagctg ctattccttc acgtttacct ctacgcannn nnnnnnnnnn
     1381 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1441 nnnnnnnnnn nnnnnnnnnn nnnnnnnatc ttttctacta attctgccaa cattggtttt
     1501 acccccatta aatttcgtat cgaattgagt tcgatttctt taagttctac tttgaacatg
     1561 ttttttagcc agagaaaata atccctgata tctgacggat cttcgtcttt aatatacatt
     1621 actatacggt ctatggcaac acgcaacttc ttttcatttc cagcattttc tatttttatg
     1681 acggccgcaa ttagatttga taatttatct ataaaagtgt cagaaatttc attttggata
     1741 atcgcaaaat actcaaagga tggtatgtaa ttagcaggta tatttttttc aatcagatca
     1801 ccaatgttac gtggaactgt ccattttttt gatccgttat ataataataa aggaaagacg
     1861 tttggcaata aacccttctt actgtttttt aatagttgat catataagag cagtatatat
     1921 tgaaacattc tgatcggtat tgccttgtct ggagtggatt gaaattccat taagatatat
     1981 atgtaggtct cgtttttgtt gagattaatc ttgtaaagca gatcactttc tcggtctagc
     2041 atctcttcag aaacaaaggt cttatcagcc agttctagat tattttccgt taagcctttc
     2101 acgaaatctt catgtacaaa agattttagt agttgcaaaa aaattctttt gttcgaaaat
     2161 aggaatttat atgcattgtc atgtggtttc gtcatcaatt tatctccttc ttgttcttac
     2221 atatataaac cgattttttc aaaaaaaggg cgaaaaaaat tgttctctat gaaaaaaaga
     2281 ttgttttcga tagattgcta gtttttgcat aatattcaaa gaaagtggat tgtatgaata
     2341 caaacaattc tttcatgaaa cagtggtttg acggtgttga agattttctg gaatcttcgg
     2401 gcgaggtggc tcgcaaagat tttttgtcgt gttgtgcgaa aaggtgttca gactcttatc
     2461 cgcttgcctt gtacaaagag gccttttctg atggtcgaag tttacctgaa gcattgaagt
     2521 atttggctaa taattttgac gggtttgatt atgctgtttt ttctgacaga gtagagctta
     2581 gttatagtca atgcggatgt gatctttata aagaggggct gatcacaagc gttaatttat
     2641 gtcaatgctc ggaggcgagc ttgctttata tctgggagca gatttatggc aagggcgctg
     2701 tagcggttac aaccaaaaag acgattatcg gtggtaacga ttcatgccta tttgagattc
     2761 gtattttaaa atgaggtaaa tatggtttat tcggtgatta tcaggttata ataattactg
     2821 attgaccttt ttttcgactg tgctattata tcatcaataa ataaatcaga ggtaatatta
     2881 tgattatcag aatgcaatgt aaatcttctt caggaaaacc attttcgggg ttccgaacat
     2941 aacatagcgg gaaatctata aactaataat cccgccctgt ttcctcgggg acccttccct
     3001 aaatgcgcgt ttgtgactta aggtcataag aatgcgtaaa cggagagtaa tgtgctccgt
     3061 tgtttaataa tgaggaaata gaaaatgaat ttaataaacg ctaacgcggt tttttatagc
     3121 aacggcagta agactatact gcgcgatgca tctttttgtg tgcaaaaatc aaaaaaatac
     3181 gctcttattg gtcctaacgg taccggcaaa acgaccctgc ttcgtctaat tacaggtgag
     3241 cttgaagtag agtcaggaac cataacctgt aaggagtccc taaagtggag ccttgttccg
     3301 caagaggcag agccgggcga tggtcgaaca atacgcgaat atatgctatc gccgcttgct
     3361 cctgtgataa aaaagatggc tatgcttgaa aagcagatgc agacggcacg tgatgaagag
     3421 cttgcggatc ttctggttga gtatcagaag gtgcaggatc tgtttgaaaa aacgggaggg
     3481 tacggcgctg aggataacgc tttgaatctt ttggcaagcc ttggtgtgca aaatgaccct
     3541 gaccaaattg tggatacact ttcgggaggc gagcagagcc tgcttttttt tgcacgagcc
     3601 cttgcaggtg acccagagct tttaattttg gatgagccgg gaaaccacct agattatctt
     3661 gggcttgcct ggcttgagag ttttttgcaa aacttcagag gtgcagtaat aattgtgtcg
     3721 cataacagat atatgattaa ccgaatctgt gatgagatat ggcatttgca aaacggcagc
     3781 ttaaccgttt ttaacggtaa ctataacagg tttaaaacca gttaccttcg tgacggagca
     3841 ataaggcaaa acgagtatca aaaggcgcaa aaagagatag gcgaacttaa cacgcgaatt
     3901 tcgcgactgc agtcgattgc tcgatcacag tataaccctc cgccgggggt gctcagcgaa
     3961 cttaaaagtc ttatggcaaa gaaacagcgt atagagcaga acatgccgga taagcaggat
     4021 atactcgcgg ataccgttaa gataagtgtt aacgcgcaag cggtacaggc agatatagcc
     4081 tgtgaggtta aaaatttgtg tttgagcgcc ggtgacacat ttttggcaga taacgtgagt
     4141 tttaccttac attgcggtga gcgagccgcg atagaggggc taaacggcac gggaaaaacg
     4201 accttgctcg aaaaaattgt aaaagagggc agcagggatt cagacattat aagaataggt
     4261 cccagtcaaa agcctgggta tctgagccag aagccgcaat ttgctactga tgcccagaca
     4321 attgccgacg aggtaaagag ctgggggcag ctttcgcgcg atgaagttta cgatacgata
     4381 aagaagtttc tgtttactta cactgatctt gataaaaaac tttcagtttt atcgggagga
     4441 gaaaaaaaca ggcttcagct tgcgcggctg atctattgca aggcaaattt tcttgttttg
     4501 gacgagccca ctaaccatat ggatattcag actcgtgagg ttatggatga ggtattaacc
     4561 ggttttgcgg gaacaattct ttttgtgtcg catgaccgct attttttaga cgcggtagca
     4621 caaagggtta tcgagattgc cgacaagaag atcacggtgc atcagggtaa ttttaccgac
     4681 ttttttaaaa aacggtatcg cactctaccg cggctttcgg gatcggtaac aacacgagct
     4741 aagaagcgta atccgtataa cgactctgca tccaaaagcg cgtctggcca aaaagagaaa
     4801 cagctggaaa accggatcga agagatggag caggagatcg cgtgtttaac acacgagctg
     4861 gagctttgcg tatcacaaaa aaacagcgca aaaggtcgac agcttgcctc gcgccttgac
     4921 aaactagaca acgccctgac acgcctttac gcagaatggg attcagtttg taaaatatag
     4981 catatcggta ttttttttaa aatactgttt tatgtaatta ctatttaaag tcccccttct
     5041 ggggggacct gacttttacc cggaaatctt atcaaaatat tggccgattt agataaacct
     5101 gatccggaga ttgaccgtat atgggctgaa gaagcaagaa atcgttgggc ggcgcataaa
     5161 gctggccgta ttccagctgt ttcatattcg gatgtgatgg gaaagtatcg ccgtatatga
     5221 aagttctttt cttaaacagg attttttatt ttttataagc aggttatatc aagtataaaa
     5281 taattaaatc ttattcccag tcttcaattt cgagtttaat aattctggaa aattcttttg
     5341 tattatgagt aacaagaatc aaatcattca gtaacgctat actggcaatt tgcaaatcaa
     5401 aaggtccgat aggttttcct ttcttttcta actgagctcg aatatctccg aaaatatctg
     5461 aagcttcatc atcgaaaggg atagaaggga aaacattaag aaaattaatc aacttgtcat
     5521 aagttttttt aaaggatttg cttttataat ctcctgcaaa taattcagct ctaacaactg
     5581 agcagattac aatatcatca ggactgaaag aagagatttt tttaactatt tcatcagatt
     5641 gaccatttaa atacttaatg catacgttgg tgtctaaaag ataagtcata gaagctgctc
     5701 tctttgggga aaatcacctt gaggtagtct ttcgatttga tcatttctta aacaaccata
     5761 agtattttca aaaaaatttt caggccataa tcgttttgag ttattcttcg gctgtaaaat
     5821 aattaccacc tccacatcaa cttctttttc agttgttggg acttttaagt ttaatatacc
     5881 atcacgtcct attctggatc ttaagtttat cgttttcatt gcaatctttt ctctcatttt
     5941 atattattaa ctatagacta ttgctataca tgaatttttt caatcaaaaa attattataa
     6001 atttgctttt tcacattaga gtattttaga cacaattaat agcatggtca ttttagccag
     6061 ggttaagtat attgcaacac ggcggcgagg ggcgcaaaaa tatcctacat ctacggttta
     6121 ggggacgata cgtttgtggt agtagcggtc gcgcatttgc atcgtgaacc gcgttattgg
     6181 tcagatcgca tttaatagaa cgcgagttgc atacgtattc cagtatcatt cagaccgctt
     6241 tatgcctacg gttaataatt ggttgacaac ttgataaaag cagttattat gaatggatga
     6301 aaacattacc ggttggcgaa tttaaaacac atttttctga gatacttgac tgtattaaaa
     6361 acggtgaaga gattgcattt tcatatggca aagggaaaga gaaaattgct gtacttgtac
     6421 catatgcaaa atactctaaa aagcgtaaac gaaatctggg attattacaa aataaggcat
     6481 catttgaaat acaatctgat tttaaaatca ccgatgacga atttttagta ttatgaagta
     6541 tctgattgat actcatgtat tactatggag tctttttgat acgtcaaaat tatcaaaaaa
     6601 agtatatggg atcttattag atatagaaaa tgaaatttat gtgagcaaca ttactttttg
     6661 ggagatttcg ttgaagtatg gcattggaaa gttgtcattg aaaaatattc ttcctagctt
     6721 ttattcattt taaaagtact gttctgtgca ataactattt aaagtccccc ttctgggggg
     6781 acctgacttt tacccacaaa tcttagtcta tatgccccca acccccaaag ggggcttctt
     6841 attcatcttt tttcaataat gaagttagta ttttattaat taccgtacaa ttattactca
     6901 tgacttcttc tactgaaaac cgaagagttg taattccaat actttctaga aatttatcac
     6961 gataattatc ataatctacc cgttttttat gaatatctcc atcaatttca attgctaaat
     7021 atgctttatg acaataaaaa tctaatatgt atcgggaaat tggatgctgg caacgaaatt
     7081 tgtaaccatt aagctgtttg tttctcaatt gtgaccatat tttttgctca gaaacagtta
     7141 ggttttgtct gttttgtctg gcaaattcga taacatattt aggtgtattt acctttgttt
     7201 cattatatag catatcgata ttttttttat aatattgttc tgtgcaataa ctatttaaag
     7261 tcccccattg ggggatttag ggggctatct gattttaaaa tcaccgatga cgaattttta
     7321 gtattatgaa gtatctgatt gatactccag cgatggatta aaaatagtct ggtaagcaga
     7381 attattattc cacatctatg acatttaggt tcttcttaaa ccgatttttt ttcaggtgtt
     7441 tttcaaagtc atcaaaactc attatctctt ttggttcaat cttaaccttt gattcttttt
     7501 cgtagtggtt aattgttaac attccatccg agtcaaagaa atcataaaca aaagggggaa
     7561 acagttcttc ataccagctt agctgaaaaa agctgttgca tgaactgccc ttttcgctta
     7621 catcaataag caggccggtg cttttgttgt catgttcgct accgaaaata atatattcta
     7681 caaacgattg attagcgata ttagcattat tagcattatt atctgtttta aaatctatgc
     7741 tgtattgatc aataaatgga gataagttat agctaacaaa ttgtgactcg agtaaaatat
     7801 tttttagttt gtcggtatca ttttcataat agaagttccc tgtcagatta ttctctttaa
     7861 aaaagtcaaa taacacatta agaaaattgc taagattttt gtattctttt aatttcatgt
     7921 tttattatcc ccaatacact tgtttgaata gcagagttat caatacgaag tagtatttta
     7981 agtacactta tcacaaaaca tgcaaattgt caaaagtcaa gctgtgaagg tattattatt
     8041 tatgttattt gtaaaaaatt attcacttcc gcacggtttt gcttattcga ataatgactc
     8101 acgcgaagcc gcgaaggcgc gaaggaagga tgaaggggca cgcgaaggcg cgaaggcgcg
     8161 aagaaaggat aaggaagggt cacgcgaagg cgatagggcg cgaagaaaag atgaagaagt
     8221 gttacgctaa gccgcgaaga gcggggtatt tgttgtatct tgtaacgctt tgttggtgtt
     8281 gattaaaggt taatttatag gtaatctcta taacaagcga tacttttgag cgtttttttg
     8341 aagttcccaa atttaaagtc ccccattggg ggatttaggg ggctcagagt tttgacattt
     8401 tcaagaaaat agaattagtt gttacttaaa tagtcaataa acaataatta ctcaactcga
     8461 ttagacaggt gctgattaaa tagccacttg attacttctg gatctgaata tgctggggag
     8521 ctgtgtccca tacccgcata atggcttcgt ttaaaaattt caactccggc caggcttagg
     8581 ctggtggttg tgcattcata acccgagacg gtctttgttt ctacagactc tgtggctttg
     8641 gagctgctgt ccagcccttt gacgtaagcg tacgcctggt tagatattcg ccgctcatca
     8701 tcggggtctt cgcttaataa tccgttacca tatccgaagt cgtatgaatc gctcaggcct
     8761 acgtgatacc agaccgaggt tttatctgcg atggcctcgg ataattcagg attacttccg
     8821 ccggcacagc ggactatacc agcaacggtt aacctctgtt ttgcttcaag attagaagca
     8881 aaaggatatg atccggaacc cccagcggag aatcccgcca gatagattcg atctgtatcg
     8941 attctatagt cgtcaaccag ttgtgcaatc atggcgtagg tccagtcgtt gttggaagct
     9001 gcattcagac aaaagcaggg atagtcttgc attctttggg tatcgcccgc gaccaggctt
     9061 ccagagtaat ggacattaat catgagagga tagctgcgat ttgagttatg gtctttgtca
     9121 tagttccagg gaagcataag ttcgtaactt tgtgaggtcc cgttgtcgtc ataggttttt
     9181 gtttcgtagg aaggtttatc ggcctcatca aagtcttctt cgattggatc atgagtaaac
     9241 ggctcgtcgg tagaggagtc gtcagaatca tcacacgaaa acagaaagag aaaagcgatt
     9301 ataaatacgg atattgtcac agttaattta atggggtttt tcattgtatc tatcttctcc
     9361 agattattta ataatatgtt accaaaaaaa gatttttaaa ttgagtgtga aaaatcgtgc
     9421 tggtcagttt ttactttgca ataatcccga gaacacctgc cggccagctt ttgtaagtgt
     9481 accataaggc atgtattaaa tgaaatgcat aagtagtaga attaaataat acagtatgag
     9541 gacagtacac taggtttctt tttggtcaac tattaattta taagatagat acaatgtttt
     9601 tgtaatatga agataagcat tgtggggctt tgttcctcga ataatgaatc acgcgaagcc
     9661 gcgaaggcgc gaaggaagga tgaagaaggg gcacgcgaag gcgcgaaggc gcgaaggaag
     9721 gatgaggaag gggcacgcga agccgcgaag gcgcgaagga aggatgaaga aggggcacgc
     9781 gaaggtgtga aggtgtgaag taaggataag gaaggggcac gcgaagctgc gaaggcgcga
     9841 agtaaggata agg
//
LOCUS       DAOTGZ010000090         9764 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_3064
            NODE_8273_length_9764_cov_4.139046, whole genome shotgun sequence.
ACCESSION   DAOTGZ010000090 DAOTGZ010000000
VERSION     DAOTGZ010000090.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868088
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 9764)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9764)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:22:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,785
            CDSs (total)                      :: 2,751
            Genes (coding)                    :: 2,732
            CDSs (with protein)               :: 2,732
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 12 of 19
            Pseudo Genes (internal stop)      :: 5 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9764
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8273_length_9764_cov_4.139046"
                     /isolate="AMR_MDS_3064"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..251
                     /locus_tag="P5123_07320"
     CDS             <1..251
                     /locus_tag="P5123_07320"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRX16106.1"
                     /translation="SGLVQQKIRAATSGPLYVYIVATFIGKLCSVFTVCVKRIANVSL
                     LNQESCRLNQEATMRYSKEFKRSTVGRLHVGTIYDNFF"
     gene            complement(248..682)
                     /locus_tag="P5123_07325"
     CDS             complement(248..682)
                     /locus_tag="P5123_07325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002672065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system HicB family
                     antitoxin"
                     /protein_id="HRX16107.1"
                     /translation="MKYHFKVHTAPDGLWAECLELKGCVTQSEINTKDDLYKNMTEAL
                     NLYLEEEVDSNTIDPLPDMTLTGKTNVVEVPVDPRIAFSVLIRHERKKRGLTQEEMRK
                     KLNMKGIYSYQRLEKRIDPRLSTIAKIREVFPDISLDQLFCC"
     gene            complement(692..877)
                     /locus_tag="P5123_07330"
     CDS             complement(692..877)
                     /locus_tag="P5123_07330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002685690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003729 - mRNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system HicA family
                     toxin"
                     /protein_id="HRX16108.1"
                     /translation="MPISGKEMLKRYLDAGWIKVRHKGTSHVRVQKGSKKETIPMHKE
                     LKKGLEAKLLKRLKEVR"
     gene            1490..2065
                     /locus_tag="P5123_07335"
     CDS             1490..2065
                     /locus_tag="P5123_07335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020586097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid pRiA4b ORF-3 family protein"
                     /protein_id="HRX16109.1"
                     /translation="MNERFYLLKIELLDIEPAIWRRFVVPASITLDRLHDVIQIVMGW
                     TDSHLHEFTIGKKRYTEFPESKEDGLTCGRYRLGDLIKQNGRGFHYLYDFGDYWEHEL
                     VIEHNRYFDPELREEIVCLDGSRSCPPEDVGGVPGYYKFCSAMQNINHEEHNDYMQWF
                     GKDFNREYFDCDTVNLELLKYLRWSRTRYKN"
     gene            2087..2434
                     /locus_tag="P5123_07340"
     CDS             2087..2434
                     /locus_tag="P5123_07340"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRX16110.1"
                     /translation="MFGKLSSEIEQEINNKGYHILRKSFARKKMYPNKFPDYDEIEQI
                     LIGKIYTLRLFVKHKRNGTERIDGGMIDVKIIKNNNEEYSGELLTLLPPNFPIKKGDH
                     LKICKDEILFEQK"
     gene            complement(2719..3384)
                     /locus_tag="P5123_07345"
     CDS             complement(2719..3384)
                     /locus_tag="P5123_07345"
                     /inference="COORDINATES: protein motif:HMM:NF024206.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ankyrin repeat domain-containing protein"
                     /protein_id="HRX16111.1"
                     /translation="MKFLKLLLLFFIIISGLYSCKMDPEVLNSKLIQAVMSMKADRVE
                     RYLEKGADPNTVLQDGETTVLDMALYKNEQHIFIMLAEAGADISTADSDGTSVLTRAA
                     ASNLTEAVSYCIEHGNDVNFINNNELKTTALMEAAARNAADSIKILLENGADTNILDI
                     NKSSALHFATLKGSYEAVVALVESGADLSIRDMNGDTPLKIAETEGHDKIVEYLKKSG
                     GKN"
     gene            3673..4314
                     /locus_tag="P5123_07350"
     CDS             3673..4314
                     /locus_tag="P5123_07350"
                     /inference="COORDINATES: protein motif:HMM:NF013077.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HRX16112.1"
                     /translation="MDNFHSLAETRRSIRKYTEDDVPVNIVVDCIKTAVAAPSGCNSQ
                     CWKFVVIHNKKVIEEASQIVRDKHKEVLDSLGMNYDKSYLEARNKMLTFFTKAPVCVA
                     VFMTKLDYYDKQFEQAFINNGYTSKQLMETYGNPDVLSIGAAVQNFLLAVHEKGYGAC
                     WMNDPVIARKELSQLFGMNDGEVLMSLIPVGVSAYTPTAKKQKELRDVVKVIE"
     gene            4533..5546
                     /locus_tag="P5123_07355"
     CDS             4533..5546
                     /locus_tag="P5123_07355"
                     /inference="COORDINATES: protein motif:HMM:NF020650.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HRX16113.1"
                     /translation="MKSKLKKAIYISLLIIASVIIYFGYNSGMKREEQASFVIGTNPN
                     LPTLTLYTSSAITTPQLAFWAAVNRGEFSEHFNLIVRLYRSIDEIQNLMLAGKGDIWI
                     AHTGGFAMAFNRGAPVTVAVITAWKKFYIVTSDATYHSLEDLAGKTVAYSPPGSPGFS
                     LFKNLVSADIPDIRLQAYHGRELQMLLLNGKVETAILPEPIVSMMLIKNSKLRIVGNM
                     EELYGKKYGLEPLLPLAGIGFNRLTAEKYPGIANLIQDSVVRNSKDIQSDKKLAISNL
                     PDSFRSEIPEDVAVMSLERDVILARPAYEVYALINDYLSITIPDYNKETSKISDKEFL
                     WQN"
     gene            5534..6280
                     /locus_tag="P5123_07360"
     CDS             5534..6280
                     /locus_tag="P5123_07360"
                     /inference="COORDINATES: protein motif:HMM:NF012738.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HRX16114.1"
                     /translation="MAKLATAIPFGVLVGLWFMVSLIYGEQLVPAPSSVMLEGLRLMS
                     EIKNWEEISITLFRGVVGVTISFVIGFVIAVPCGFSQKAMKILSPFIVASQGCPPIIW
                     ISLLMVWAGMSSVVPLAVVIITLVPVVFFNIAQGVASLEEHLFSMAKLYHVSRPRMVI
                     DILLPGIRPYVFSSLSYSLSVAWKVTATAEFLGSSSGIGSRLYWAYRYLDMTQLFFWS
                     LVLILIGFSIEMLIIQPLREYRKTPWVSSK"
     gene            6277..6897
                     /locus_tag="P5123_07365"
     CDS             6277..6897
                     /locus_tag="P5123_07365"
                     /EC_number="7.6.2.14"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HRX16115.1"
                     /translation="MKKMIEAVNISKSFGKQQVFADLSISANKGEWLCVYGDSGCGKT
                     TLINTLAGLTMPDNGTVSRYTERIGFAFQDDRLLEWKSSLDNIVFVLRSQYERSVCIR
                     ESKKWLDKVGLGNFAERKPPQLSGGMRRRLNIARALAVKPDILFLDEPFAFLDKANAI
                     TVRNLIVDAVKKMRTTVIMVTHSLDELKDLDYRLIDIGTSKVDSLS"
     gene            complement(6909..8078)
                     /locus_tag="P5123_07370"
     CDS             complement(6909..8078)
                     /locus_tag="P5123_07370"
                     /inference="COORDINATES: protein motif:HMM:NF012488.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase family protein"
                     /protein_id="HRX16116.1"
                     /translation="MSLYLDNNATTALHPAVKEAIIEFLDSYGNPGSMHESGRKVKNR
                     LDTAREEIASFLGTTPSKLHFTSCASESNNTIFKSLLLHDFGFKPHIITSAIEHPSII
                     TTARFLEKNGVDVTYLPVTKDGVIEISALENAITDRTVLVSIMHANNEIGTVQPIEDA
                     AQLLAQKNIFFHSDMVQAPGKIIFNLDSMNIDAASFSAHKMHAPKGIGALYLKNSKPK
                     NFPFKPLIHGGHQENGLRAGTENTIGIIAWAAACKTIKSDLQKEINYLKNLRDSFESI
                     IKQTITDVVINGESATRKPCTSNISFKYVEGESILLRLDMEGIAVSTGSACSTGSLDP
                     SHVIMALHNDPETAHGSIRFSFSRENNMEDVKTTAAVLKKTIDFLRSISPLTPKG"
     gene            complement(8081..8716)
                     /locus_tag="P5123_07375"
     CDS             complement(8081..8716)
                     /locus_tag="P5123_07375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010933650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster assembly scaffold protein"
                     /protein_id="HRX16117.1"
                     /translation="MDKNQWAYSENVKKHFMNPQNILQIEEDKYPADGKGMVGSPACG
                     DMMMVLIKVANNRISDFKWKTFGCASAIASTSALSELVLKDGGMTLEEAYKVTPEDII
                     RELGGLPSNKIHCSVLGDKALREAIDDYYNKTNQKNPSQESVSRVVCDCKNVTEEDIR
                     MEVLEGVTDFEKMKEVTGASTVCGKCEQDVRDTMKKFVEQYYKDDIYNPGE"
     gene            complement(8823..9254)
                     /locus_tag="P5123_07380"
     CDS             complement(8823..9254)
                     /locus_tag="P5123_07380"
                     /inference="COORDINATES: protein motif:HMM:TIGR00738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Rrf2 family transcriptional regulator"
                     /protein_id="HRX16118.1"
                     /translation="MRLSTRSRYAIRSLLDLIYYYKGSPVSIKEIAERQQISERYLEN
                     IFHDLRKNKIVNSIKGKNGGFMPGTDLKEVKLLTIIEILEGPVKIVDCTAEPSDCPRI
                     EKCSSHPVWLSLNNIFRDTLSKIKLGDIKGCEEGSIQELKI"
     gene            9566..>9764
                     /locus_tag="P5123_07385"
     CDS             9566..>9764
                     /locus_tag="P5123_07385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019560120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polynucleotide adenylyltransferase PcnB"
                     /protein_id="HRX16119.1"
                     /translation="MKTVEYKLELDEISARRITKLTQTFFEAGYECYMVGGSVRDLVL
                     GLTAYDFDFATNAKPQQVIKLF"
BASE COUNT         2845 a         1756 c         2019 g         3144 t
ORIGIN      
        1 taagtggttt agttcaacaa aaaatacgtg ctgcgacctc tggtccccta tatgtctaca
       61 tagttgcgac ttttattgga aaattgtgca gcgttttcac tgtgtgtgtg aaacggatag
      121 ctaacgtatc cctcttaaat caggaaagtt gccgcctcaa tcaggaggca acaatgagat
      181 attcaaaaga atttaagcga agtactgtag gcaggttgca tgtgggtact atttatgaca
      241 atttcttcta acaacaaaat aattgatcta atgatatatc cggaaacact tcccttattt
      301 tcgcaatagt ggacagtcgg ggatcaatcc gtttttcaag tctctgataa ctgtaaattc
      361 ctttcatatt gagttttttt ctcatttctt cttgggttaa tccgcgtttt tttctttcat
      421 gccgtatgag tactgaaaat gcaattcttg gatcaacagg gacttcaaca acatttgttt
      481 ttccggttaa tgtcatatct ggaagaggat caatagtatt cgaatcaact tcttcttcaa
      541 gatacagatt taatgcttct gtcatatttt tataaagatc atctttggtg tttatttctg
      601 attgtgtaac acatcccttt agctcaagac attcggccca gagaccgtca ggagcagtgt
      661 gtactttaaa atgatatttc atggtaaccg cctatcgaac ttctttgagt cgttttaata
      721 attttgcttc cagtcccttt tttagttcct tgtgcatggg gattgtttct tttttggagc
      781 ctttctgaac acgtacatga cttgttcctt tatgtcggac ttttatccat ccagcgtcaa
      841 gataccgctt tagcatttct tttccactta ttggcataca tcccctttga cactaacagt
      901 ataccaacta ttgttagtaa tgtcaatata aaaaaggcca ggttcatccc accctaaatt
      961 aggaaagttg tcgcttcgta gtttagtgaa aaacctatat ggccctatat gtcagcatag
     1021 ttgaggtttc aaaaaacaag gagaaattca attattaggt gcagtttagc atttaaggag
     1081 tcatatatca aaaaagtatt gccgtcatca aatttacaaa ttaagtatct cgcagacgag
     1141 tcgatgccgg gtgcagtcag cgtttcgcgg aaacaggtta catacaacaa gtcgccactc
     1201 aaagaagcac gaagagaact gcgtttttac ggaacgtctg tagaagaaaa gttcttgaat
     1261 tggtaaagat acaggtgata atcttgacta gtctatgata attttaaaat gaaattagaa
     1321 attactgtag acgatttacc tgtgctgaaa tatagtaggg atgaaggcat ggtaactcaa
     1381 atgtatctag tttaacggat gataaactta ccgcaagggc gttttatata tataaagcat
     1441 agcaaatatt aaaacactta aaataatgaa attaaagatt ataaaaaaaa tgaacgaacg
     1501 attttatttg cttaaaatag aacttcttga tatcgaacca gcaatatggc ggcgttttgt
     1561 tgttccggcg agtataactt tagaccgtct gcacgatgtt attcaaatcg ttatgggctg
     1621 gacagacagc catctccatg aatttacgat aggtaaaaaa cgctataccg agttccctga
     1681 atcgaaagaa gacggactta catgtggcag atatcgtctt ggtgatctta tcaaacagaa
     1741 tggccgcggt ttccattatc tttatgattt tggagattac tgggagcacg aacttgttat
     1801 tgagcataac cgctattttg atccagaatt gagagaagag attgtttgtc ttgatggttc
     1861 acgatcctgt cctccggaag atgtaggggg cgttcccggc tattataaat tctgtagcgc
     1921 aatgcaaaac ataaatcatg aagagcataa tgactatatg caatggtttg gtaaagattt
     1981 taacagagag tattttgatt gcgacacggt aaatcttgag ctattgaaat accttcgttg
     2041 gtctcgtaca agatataaaa attaaaaaac agtaaaggat atagatatgt ttggaaagct
     2101 atcaagtgaa attgaacaag agattaacaa taaaggatat catattttaa ggaagtcatt
     2161 tgcaagaaag aagatgtatc caaataagtt tcctgattat gatgaaattg aacaaattct
     2221 tattgggaaa atatacactt taagattatt tgtcaaacac aaacgaaatg gcactgaaag
     2281 aatagatggc ggaatgattg atgtgaaaat aatcaagaac aataacgaag aatattcggg
     2341 ggaattattg acattattac ctccaaattt tcctattaaa aaaggagacc atcttaaaat
     2401 ttgtaaagat gaaattcttt ttgagcaaaa ataaaaaatc aataatcgct gatatctaaa
     2461 gtcccaatat ttcagtatag tttgcattgg taatattatt gattgtatcg agatagctaa
     2521 aaatgaagaa tactaatggc tacttgccga accggaacgg ttaagtagtt ttcttgtgcc
     2581 aaccaaccag ccccagccgg gaatctggcg actgcagttc ttatatggaa aaataaatgc
     2641 tgatataaaa aaccggaaag ttttaaaaaa agtgtgcaac agggaaagta aaaataccat
     2701 ccctgtgatc gtacaaaatt agtttttgcc accgctcttt ttcagatact ctactatttt
     2761 gtcatgccct tcggtttcag caattttaag aggtgtatct ccattcatgt cgcggattga
     2821 aagatcggct ccagattcaa ccaacgcaac cacggcttcg taactgcctt ttaaagtcgc
     2881 aaagtgcaaa gcgctgcttt tattaatatc aaggatattg gtatcggcac cattttccaa
     2941 tagaatttta atagaatcgg cggcatttct ggctgcggct tccataagtg cggttgtttt
     3001 tagttcgttg ttattaataa aatttacatc attaccgtgt tcaatgcaat aagaaaccgc
     3061 ttcggtcagg ttggacgcgg cagctcttgt tagtacagat gtgccatcag aatcagctgt
     3121 gcttatatcg gcgccggctt ccgctaacat aatgaatata tgctgttcgt ttttatataa
     3181 ggccatgtca agtactgtag tttccccatc ctgtaaaacg gtattggggt cggcaccttt
     3241 ttccagataa cgttcaactc tgtcggcttt catgctcata acggcctgaa taagcttgct
     3301 attcagaact tcgggatcca ttttgcaaga atagagacct gaaatgataa taaaaaatag
     3361 taatagtaat tttaaaaatt tcataattcc tcctttgttt cggtttaagc tgaataacaa
     3421 taatcatcaa gtatttttat atcaatgtca aggctctttt accattatcg cttgtcacac
     3481 aaggagaact tgccataatt gccgtttaag taacatgtgt gccgtttaaa gccagattaa
     3541 aacggttatt aaatttcttg ttaataaccg gtgttttgaa ttattcttga tagctgttcc
     3601 ttaaaatgtt tacagataca tatatgttcg tgaatatagt atcataataa taattactat
     3661 ttaggagtag gtatggataa ttttcattca cttgcagaaa cgagaagaag tattcgtaaa
     3721 tatacagagg atgacgtgcc agttaatata gtagtggatt gcattaaaac agcagttgca
     3781 gctccgagtg gttgtaacag tcaatgctgg aagtttgttg taattcataa taaaaaagta
     3841 atcgaagagg cttcacagat cgttcgcgac aaacataagg aggttctaga ttcgcttggt
     3901 atgaattacg ataaatccta tcttgaggca cgaaacaaga tgctcacttt ttttacaaaa
     3961 gctccagtat gtgttgctgt ttttatgacg aagcttgact attacgataa acagttcgag
     4021 caggctttta taaataatgg gtatacaagt aaacagttga tggagactta cgggaatccc
     4081 gatgttttat caataggggc ggccgtgcag aattttttgc ttgctgttca cgaaaaaggt
     4141 tatggcgcct gttggatgaa tgatcctgtt attgcccgga aagaattgtc gcagctgttt
     4201 ggaatgaatg atggtgaagt ccttatgagt ctgattcctg tcggagtcag cgcgtatacg
     4261 ccgactgcaa aaaaacagaa agagctgaga gatgttgtga aagttattga ataataaaat
     4321 atttctaacg aagaaatttt ttaagagaaa tatgttaaaa taatatctgg tgtttaacag
     4381 aaattcttga tgaaaacaat cggactattt gacaatataa aggggggtac cttaaatgat
     4441 cctataataa acggttcatt ttggtgagaa tgatgttttt acaagccact tggataaggg
     4501 tatttcagat cggatcgtgt tgaggttatg gtatgaagag taagttgaaa aaagcaattt
     4561 atatatcact gctaattatc gcttcggtga taatatattt tggttacaat tcaggaatga
     4621 aaagagaaga gcaggcctcc ttcgtaatag gaacaaatcc gaatcttccg acgctgactc
     4681 tctatacttc atcggcaata actaccccgc aattggcatt ttgggcggct gtaaatcgtg
     4741 gtgagttttc agaacacttt aatttgattg taaggttata ccgatctatt gatgaaattc
     4801 agaacttgat gcttgccggt aaaggcgata tatggattgc ccatacaggt ggtttcgcga
     4861 tggcatttaa ccgtggggca cctgtaacag tagcagtaat aactgcttgg aaaaagttct
     4921 acatagttac aagtgacgct acatatcata gtttggaaga tttggccgga aaaactgtgg
     4981 cttattctcc gcctggttca cccgggtttt cactttttaa gaatcttgtc tctgccgata
     5041 ttcctgatat ccggttacag gcttatcatg gaagagagct gcaaatgctt cttttgaatg
     5101 gaaaagtgga aacggccata ttacccgaac cgatagtatc catgatgctt atcaaaaatt
     5161 ccaaactgcg tattgttggc aacatggaag aactgtatgg taaaaaatat gggcttgaac
     5221 ctcttttgcc gttggctggc atcggtttta acagattaac agcggagaaa taccctggta
     5281 ttgcaaacct gatacaagat tccgttgtgc gaaactctaa ggatatacaa tctgataaaa
     5341 aacttgcaat atcgaacttg cctgattcgt ttaggtcaga gatccccgaa gatgtagccg
     5401 taatgtcgct tgagagggat gtgatacttg cccgtccggc atatgaagtt tatgcgctaa
     5461 taaatgatta tctttcaata acgatacccg attacaataa agagacgagt aaaatttcag
     5521 acaaagagtt cttatggcaa aattagcaac agcgattcct ttcggggtgt tagtcggcct
     5581 ttggttcatg gtctcattga tatatggtga gcagcttgtt cccgcacctt cttcagttat
     5641 gcttgaagga ttgcggttaa tgagtgaaat aaaaaactgg gaagagatca gcatcacgct
     5701 ttttcgtgga gtagtgggcg ttacaatttc ttttgtgatt ggctttgtta ttgctgtgcc
     5761 atgcggattt agtcaaaaag ctatgaaaat tctttccccg tttattgtag cctcccaagg
     5821 gtgtccacca ataatctgga tctctctgtt gatggtatgg gctggaatga gttctgttgt
     5881 gcccttagct gttgttataa ttacactggt accggtcgtt ttctttaaca tagcacaggg
     5941 tgtagcatct cttgaagagc atcttttttc catggcaaag ctttatcatg taagcaggcc
     6001 gcgtatggtt atagatattc ttttaccggg aatacgcccg tatgtttttt cttcactctc
     6061 atattctttg agtgtggcct ggaaggttac tgcaacggcc gaatttctag gatcatcttc
     6121 aggcataggc tcgcggctct attgggctta tcgctatctt gatatgacac aacttttctt
     6181 ctggtcgctg gtgctgatac ttattgggtt ctcaatcgaa atgttgatta ttcaaccatt
     6241 acgggaatat cgcaaaacgc catgggtgag ttcaaaatga aaaaaatgat agaagctgtt
     6301 aatatcagca aaagttttgg caaacagcag gtctttgcgg atctctcgat cagtgcaaat
     6361 aaaggggagt ggctctgcgt ttatggagat tcggggtgtg gaaaaacgac tctgataaat
     6421 acacttgcag gtttaacaat gcccgataat ggtacagtta gtcgctatac cgaaagaatc
     6481 ggttttgcat ttcaggatga tcgtttgctt gagtggaaaa gctctctgga taatattgtt
     6541 tttgttttgc gttcacagta tgaacgatca gtttgtataa gagagagcaa gaagtggctt
     6601 gataaggttg gtcttggtaa ttttgcggaa agaaaaccac ctcagttaag cggcgggatg
     6661 agaagacggt taaatattgc cagagcgttg gctgttaaac cggatattct gtttcttgac
     6721 gaaccctttg cctttttgga taaggctaac gcgattactg ttcgtaatct gattgtagat
     6781 gctgtaaaaa aaatgagaac aacagtgatt atggttactc attcacttga cgaactgaaa
     6841 gatctagact accgactaat cgatattggt acgtcgaagg tggacagtct atcttgaaag
     6901 aaaaacaatc agccttttgg ggttagcgga ctaatacttc ttaaaaagtc tatggttttc
     6961 tttaatactg ccgctgttgt ttttacatct tccatgttgt tttcacggct aaagctgaaa
     7021 cgtatcgagc catgggctgt ttctgggtca ttatgcaatg ccatgattac gtgtgacgga
     7081 tcaagggatc ctgtagaaca tgcgcttcct gtagacactg cgattccttc catatcaaga
     7141 cgcagcagta ttgattcacc ctcaacatat ttaaaggaaa tatttgatgt gcatggtttg
     7201 cgggtggccg attctccgtt tataactaca tctgttatgg tctgtttgat aatgctttcg
     7261 aaactgtctc taaggttttt tagataatta atctcttttt gaagatccga ttttatggtc
     7321 ttacaggccg ctgcccaagc aataatgcca atcgtgtttt cggtccccgc gcgaaggccg
     7381 ttttcctgat gcccgccatg gatcagcggt ttaaaaggga aattttttgg cttactgttt
     7441 ttaagatata gagcgcctat accttttggt gcgtgcatct tgtgtgcact aaaagaggcc
     7501 gcgtcaatgt tcatcgaatc aaggttaaaa ataattttgc ctggagcttg taccatatca
     7561 gaatgaaaga agatattttt ctgagctaac agttgtgcag catcttctat tggttgaact
     7621 gttcctatct cattattagc gtgcataatg gatacaagaa ctgttctgtc ggttattgca
     7681 ttttcaagag cagatatctc tataacgcca tctttggtta cgggaagata ggtcacatct
     7741 acaccatttt tttcaagaaa tctggcagtg gtaattattg atggatgttc aatagcactt
     7801 gttatgatgt gcggtttaaa accgaaatcg tgtaagagta gggatttaaa aattgtgttg
     7861 ttagattcac tggcacaaga ggtaaagtga agcttcgagg gggtggtacc caaaaaagat
     7921 gcgatctctt cccgtgctgt atcaagcctg tttttgactt ttctgccaga ttcatgcatg
     7981 gatcctggat tgccgtagct gtccagaaat tcaattatgg cttctttaac cgcagggtga
     8041 agggctgtgg tggcgttgtt gtcaagatac aggctcatag ttattctcca ggattataga
     8101 tatcgtcttt ataatattgt tcgacaaatt ttttcattgt atcgcgtaca tcctgttcgc
     8161 attttccgca aacggtcgaa gctccggtaa cctctttcat cttttcgaaa tcggtaacgc
     8221 cttcaagtac ttccatgcga atgtcttctt cagttacgtt tttgcagtcg caaacaacac
     8281 gagatacaga ttcctggctc ggatttttct gattggtttt attgtaataa tcgtctattg
     8341 cttctcgtaa agctttgtca cccaaaaccg aacagtgaat cttgtttgac ggtagtccgc
     8401 caagttcgcg gataatatct tcgggtgtga ccttgtatgc ctcttcaaga gtcattcctc
     8461 cgtctttgag tacaagctct gataatgccg aagtcgaggc gattgcagaa gcgcatccga
     8521 atgttttcca tttgaaatcg gaaatacggt tatttgccac cttgatcagt accatcatca
     8581 tgtcgccaca tgcaggagag cctaccatgc ctttaccgtc ggccgggtat ttatcctctt
     8641 cgatctgcag tatgttctgc ggattcatga aatgtttttt tacgttttcg ctatatgccc
     8701 actggttttt atccatgttg ttctcctttg tcagtgtttg cgctgttgcc agatgacccg
     8761 ttccatttcg attcttaggt taaaaacgtc aagtctgttc ttgtaacggt gtttcatcct
     8821 ctttaaattt ttaattcttg tatgctaccc tcttcgcacc cctttatatc accaagtttt
     8881 atttttgata atgtatcacg aaatatattg ttcaggctga gccaaaccgg gtgtgatgag
     8941 catttttcaa ttcgcgggca gtcagacggt tcggcagtac aatccacaat tttaaccgga
     9001 ccctcaagta tctcgattat tgttaaaagt ttgacttcct tcaaatcggt gcctggcata
     9061 aagccgccat ttttaccctt tatactgttt acaatcttgt tctttctcag atcatgaaat
     9121 atattctcaa gatatctttc ggaaatctgc tgacgttcgg caatctcttt gatcgaaacg
     9181 ggcgatcctt tgtaataata tataagatcc agaagcgacc ttattgcgta acggctgcgt
     9241 gtcgaaagtc tcatgtctct tccccgttta tctttgatat ttttaaacaa aacacaaaaa
     9301 ccataataaa aactgtacaa aaaaattcaa ttacaaaagt acagtatttt agttatatta
     9361 tacatcaaaa ttgcaaaaag accatgtttt ttttaaaatg gtttgtacat attttattct
     9421 ttaggcaaaa ccgccaattg ttagttaaat cttttgatct tgacagataa cagggtattt
     9481 ttagtcttat ttattgaaaa atgcaatatg gcgcaagttt gataatcacc actctttcgc
     9541 ctctgcctgc ttgggatctg atacaatgaa aacggttgaa tataaacttg aacttgacga
     9601 aatatcggca agaagaatta cgaagctgac ccagactttt tttgaggctg gatatgagtg
     9661 ctatatggtt ggtggctctg ttcgtgatct tgtgcttggc cttacggcct atgattttga
     9721 tttcgcgaca aatgccaaac ctcaacaggt aataaaactc ttta
//
LOCUS       DAOTGZ010000091         9725 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_3064
            NODE_8318_length_9725_cov_4.995553, whole genome shotgun sequence.
ACCESSION   DAOTGZ010000091 DAOTGZ010000000
VERSION     DAOTGZ010000091.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868088
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 9725)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9725)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:22:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,785
            CDSs (total)                      :: 2,751
            Genes (coding)                    :: 2,732
            CDSs (with protein)               :: 2,732
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 12 of 19
            Pseudo Genes (internal stop)      :: 5 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9725
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8318_length_9725_cov_4.995553"
                     /isolate="AMR_MDS_3064"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..386
                     /locus_tag="P5123_07390"
     CDS             <1..386
                     /locus_tag="P5123_07390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010251745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="group II intron maturase-specific
                     domain-containing protein"
                     /protein_id="HRX16120.1"
                     /translation="IIEILLFLEASTMLTYREYRVLKLAQFIRGWVNYFSLADMKTLL
                     TKVDEWLRRKIRAIYWKQWKKVRTRYRMLRNFGLPEWKVHEMANCRKGTWRSALMLNS
                     VLTKEILASLGYISMTCYYTKTVEN"
     gene            complement(551..877)
                     /locus_tag="P5123_07395"
     CDS             complement(551..877)
                     /locus_tag="P5123_07395"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRX16121.1"
                     /translation="MGKKLNLIAQANYNVPSYMGEFNYFKSLDAWSKGLDLLNNSDIN
                     WTIWTYKVVSEHENWGLYNQQIPSTNCETDSYEQIYNNWSKVGSSYPNAQLIDTVKWF
                     FKPKQY"
     gene            complement(862..1560)
                     /locus_tag="P5123_07400"
     CDS             complement(862..1560)
                     /locus_tag="P5123_07400"
                     /inference="COORDINATES: protein motif:HMM:NF012377.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0071704 - organic substance metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cellulase family glycosylhydrolase"
                     /protein_id="HRX16122.1"
                     /translation="MRKIEETPLCNKILYQYLLILIFLFSSCNPTVTDDKTEWEKVET
                     VPITCDDFLKADGKILRKKKGTGDIVFLKGVNAGGYLFQEFWMTPTEYSNNIKAEIDL
                     YENLTKRFGKKSMFALVKLYQDNYWEKKDFDNCAKLGMNVIRLPFWYMNFVDFEGNLK
                     NDCFDKLDWFIKEAGYRGLYVILDFHGAPGSQNGSDHSGVDGGDSKEKASEFFFGKNA
                     SVNQKMYYMIWEKS"
     gene            complement(1625..3007)
                     /locus_tag="P5123_07405"
     CDS             complement(1625..3007)
                     /locus_tag="P5123_07405"
                     /inference="COORDINATES: protein motif:HMM:TIGR00797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0042910 - xenobiotic transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006855 - xenobiotic transmembrane
                     transport [Evidence IEA];
                     GO_process: GO:0009410 - response to xenobiotic stimulus
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MATE family efflux transporter"
                     /protein_id="HRX16123.1"
                     /translation="MQNQILEGHIYKIITKLSVPVVIGHMLMFFYNIADTIFISYIDR
                     SSETLMSGLGLVFPLFFLYIALGSGIFNGISSLVARSIGAKDEKTLSRVADSGLLLSL
                     IAAAATIIIAFLFSTPILNILAGSQISQEALGHAKDYFLYFSPGLSLMLIQMTLAGIL
                     QGEGKPQYMGISMTISTVLNIILDPVFIFVLGLGVKGAALASSISISISLLIIIMVFI
                     RQKSGLAIHWSIFKAKFSIIIEILRIGIPHTTSMIILSFSFMALNRLVGSISEIAMSS
                     YVIYGRINELIMLPAYGIGNAAMTLVGQNYGNKNYPRVYEIVKSCIKAGAIASFIFAL
                     VIIISAPLLFPLFSEVPEIVSFAIMQARIFGIGSIGISASIVIGSAFQGAGRAMPGFV
                     LTFTKSIMVLVPLSYFLVQVMGYGFQEFVYAGASVHIFFAFISILWIVLFAKKLAHQK
                     TDTELFDKHN"
     gene            3556..7854
                     /locus_tag="P5123_07410"
     CDS             3556..7854
                     /locus_tag="P5123_07410"
                     /inference="COORDINATES: protein motif:HMM:TIGR00241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008047 - enzyme activator activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydratase activase"
                     /protein_id="HRX16124.1"
                     /translation="MSKKYFIGMDLGSTTAKIVVGYPNDSGISIEYSAYKRHHAKIRE
                     TIVGILKDVREKIGNAECLVSITGSAGMGISETTGLRFVQEVVASATYIKRVQPDIRT
                     FIEIGGEDSKIIFFDDDFHADMRMNGSCAGGTGSFIDQISVLLDVPVEEFDAYASRGV
                     KVFPIATRCGVFAKTDVQSLLSHNVSKEDIALSSFHAVALQVLSTLTRGHDIESKILL
                     GGGPLTFNKYLRKAFVECIKDIPENEFVVTDNPQLIPAIGTALTCDDQNEVQYLDDLI
                     TLVEKVPEKKVESSTRTTAIPELFKDDDDYNTWLKTFEKYHVNRMDFDLYKTKDCFLG
                     IDSGSTTTKIVLSDDDGNVVATYYHNNLGDPIGTVQKGLAEISGFCKEMGFTPNIVGS
                     GVTGYGEGLIKCAFDLDHGFVETMAHYRAASHFAPDVSFILDIGGQDMKAMFIRDGYV
                     ADIQINEACSSGCGSFIETFANNMSYKVADFAEIACRSKNPYDLGTRCTVFMNTKVKQ
                     ALRDGASIEDISAGLSYSVIKNTLYKVLKLKDSEPLGKKILVQGGTFKNNSILRAFEQ
                     LTGCDLIRPDIPELMGAFGCALKAMDLYKENAYDTSFKGFEAALIIADTEKKIINCKG
                     CENHCQVTKLTFANGNHFYIGNNCERIFTNNTEVVTRGDNLVSEKRDIIFRRNLSARK
                     PRGIKMGIPRILNMFENFPFWATFFNECGIDVVLSDRSNVEIFEAGVKSVMSENICFP
                     AKIAHGHILNLVEKDVDRIFFPNVVFERDEYEGAQNTYNCPVVTGYPCVIRSSIDPEG
                     RYNIPFDSPPISFKSSRLIVRQLIKYMKTLNVREGIVKRAYKAAIHEQKATKKRLQDR
                     ARDLIAKADSTGQPLLVMAGRPYHIDKLINHGIPELISDLGAIVIPEDSIPDLNSIII
                     SDVNVLSQWAYTNRLYATAKWVSQHPTAQIVQVNSFGCGPDAVSVDEVKEILEENDKL
                     YTIIKVDEVFNLGAVKIRLRSMLEALKKKQKVVKDGSISRRNVNQAVPFSKEKTVIVP
                     HFSKYYSPLIPPLFRSFGIKMETLPLQDHDSVDYGIKNINNEICYPAILVAGDIIKAY
                     MENNYSKDDTIVLLTQTGGQCRASNYINLVNKALDRVGFGDVQVATLSTASLKTVVTG
                     REGLLLAKKMVLGILLLDQLMYMYLTTKPREIKKGTSNALHKKYMEFFNEIIVTKHYE
                     KSMDILAQAVEEFNAVEVSNDPVPRIGFVGEIFVKYNEFAHMDLIEWVLEQGAEIVLP
                     PLQGFFLHDFISRDYMHDMHIAGSVLSRLINRFIWNYVNGKVKKVDAIMKSYKRYKKP
                     HDLHKLADKTENFVSLVNQFGEGWLLTADMLSMLDEGVENIISVQPFGCISNHVVAKG
                     VEKRIKNLYPNFKLLAIDFDQSTSPANVFNRAHFMVASAKESLQKQQEKATGTE"
     gene            8016..8345
                     /locus_tag="P5123_07415"
     CDS             8016..8345
                     /locus_tag="P5123_07415"
                     /EC_number="3.6.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019557256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004636 - phosphoribosyl-ATP diphosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosyl-ATP diphosphatase"
                     /protein_id="HRX16125.1"
                     /translation="MSENSVFGRLFDVISARKKESPDHSYVARLIFKGTEKINSKIME
                     EAAEVTEAALDNDRSHLVYEICDLLFHTFVLAGSKDISLRDIETELERRFGKSGLQEK
                     AERKNNA"
     gene            8338..9204
                     /gene="hisG"
                     /locus_tag="P5123_07420"
     CDS             8338..9204
                     /gene="hisG"
                     /locus_tag="P5123_07420"
                     /EC_number="2.4.2.17"
                     /inference="COORDINATES: protein motif:HMM:TIGR00070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003879 - ATP phosphoribosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP phosphoribosyltransferase"
                     /protein_id="HRX16126.1"
                     /translation="MLNDESRVRFALPGKGRLKDPAIELLQKAGFRFRAKGRNLYATC
                     TSDNITFIFVRADDIPLLVQKGVVDMGITGSDLVIERDADVKQLLELGFGRCRLCVAV
                     KEECDSKDYSFLKNATVATSFPRITTDFFKKNNTDINIVQMNGSVEIMVALGLADAIV
                     DIVETGDSLRDNNLKVFSDIASYETVLIANHGFASKSPMTEMISRRIEGILVATRYSI
                     LEYNIDKKNLFKAEKITPGYNSPTLSPLENEGLFAVKVMVLKGDVPSVMDKLEAIGAT
                     AIIESEIKNCRL"
     gene            9298..>9725
                     /locus_tag="P5123_07425"
     CDS             9298..>9725
                     /locus_tag="P5123_07425"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRX16127.1"
                     /translation="MLWGVKIEVLAVYAGIVVLIAAVVVVIKMLRKRFAKKADEQKAI
                     VNEHKMPASIFVLEKKKGKITDAKLPKNVLDQVPAIYKLKKMPLVVAKVGPQIVTLIC
                     EENVFSQIPEHKNVNVEVAGIFIVSVKHQSNKGKAESKRKR"
BASE COUNT         3013 a         1712 c         2021 g         2979 t
ORIGIN      
        1 tcataattga aatcctcctg tttttagaag cttcaactat gctgacatat agggaataca
       61 gagtgttaaa attagcgcaa tttatccgag gatgggtaaa ctatttttct ttggcggata
      121 tgaaaacact tctaacaaaa gtggatgagt ggttgcgacg aaaaatcagg gcaatttact
      181 ggaaacagtg gaagaaagtc agaacgcgat atcgaatgtt aaggaacttt ggcttaccag
      241 aatggaaagt acacgagatg gcaaactgcc gaaaaggcac atggcgttcg gcactaatgc
      301 taaacagtgt attaaccaaa gaaatattag ccagtcttgg atacatttct atgacttgct
      361 attatacgaa aacagttgaa aactaatgaa ccgccgtgta ccgaacggta cgcacggtgg
      421 tgtgagagga cggtaggtaa aaaaataatt acctacctcc tactcgataa acttacaaaa
      481 atatacatgc taaatattac cgtgcacaaa tatcattgtt gttgcatact acattttttt
      541 atagttacgt tcaatattgt tttggtttaa aaaaccattt tactgtgtct ataagttgag
      601 cgtttggata agatgaacca acctttgacc agttgttgta tatttgttcg taactatctg
      661 tttcgcagtt tgtcgaaggt atttgctgat tatatagccc ccagttttca tgttcagata
      721 caactttata ggtccatatt gtccagttga tatcgctatt gttaagcaaa tccagacctt
      781 ttgaccaggc gtccaaactc ttgaaatagt taaactctcc catatagctt ggtacattat
      841 agtttgcctg agctattaga tttaactttt ttcccatatc atgtaataca tcttttgatt
      901 tacagaagcg tttttaccaa aaaaaaactc tgaggctttc tctttgctat cacctccatc
      961 aacccctgaa tggtcacttc cgttttgtga tccgggtgct ccgtgaaaat cgagtattac
     1021 atatagtcct ctatatcccg cctctttaat gaaccagtcc aatttatcaa aacagtcatt
     1081 tttcaaattg ccttcaaaat ctacaaaatt catataccaa aacgggagtc taataacatt
     1141 cattcctaat tttgcacaat tatcaaaatc ttttttttcc cagtaattat cctgataaag
     1201 cttaacaaga gcgaacatgg attttttacc gaaacgtttt gtcagatttt cgtaaagatc
     1261 aatttcggct tttatattgt tgctgtattc tgtcggagtc atccaaaatt cttgaaacaa
     1321 ataccctccg gcattaacac ctttcaaaaa aacaatatct ccggttcctt ttttctttcg
     1381 taatatcttt ccgtcggcct ttaaaaaatc gtcacaagta attggaacag tttcaacttt
     1441 ttcccattca gttttatcat ccgttactgt tggattacat gaggaaaaaa gaaaaattag
     1501 tataagcaag tattggtaga gtatcttgtt acataagggg gtttcttcaa tttttctcat
     1561 taatgtacgc cattatattt tttgattggc aaaaattacg ttgttcataa agacattaat
     1621 aatctcaatt atgtttgtca aataattcgg tatctgtttt ttgatgcgca agttttttgg
     1681 caaatagcac tatccataat attgatatga aagcgaagaa aatatgtaca gaagctccgg
     1741 cataaacaaa ttcctgaaag ccgtaaccca ttacttgaac aagaaaataa gataaaggaa
     1801 ccagcaccat aatggatttt gtgaatgtga gtacaaaacc cggcatagcg cgtcctgctc
     1861 cctgaaaagc agaaccgatt actatacttg ctgatattcc aatacttccg ataccgaata
     1921 ttcgagcttg cattatcgca aatgatacta tctctggtac ttcgctaaaa agtggaaaaa
     1981 gcagtggagc cgaaataatt attacaagtg cgaaaataaa agatgcaatt gctcctgcct
     2041 tgatacagct ttttactatt tcgtaaacac gagggtaatt tttattaccg tagttttgcc
     2101 ctacaagggt catagcggca ttgccaatac cataagccgg cagcattatt agttcattga
     2161 ttcttccata aatcacatag ctactcattg caatttcgct gattgagcct acaagcctgt
     2221 taagtgccat gaagctgaaa cttaatatta tcatggatgt ggtgtggggt atgcctattc
     2281 ggagaatctc gataattata gaaaattttg ccttaaaaat gctccagtgt atggcaagcc
     2341 cacttttttg acgtataaaa accatgatta ttataagcag tgatatcgaa attgatattg
     2401 aagaggccaa tgccgctcct ttaacgccca aacctaaaac aaatataaaa acaggatcaa
     2461 gaatgatatt aagcacagtg gatattgtca ttgatatacc catgtattga ggtttgcctt
     2521 cgccttgtag aataccagct agtgtcatct gtatcagcat tagtgaaagc cctggagaaa
     2581 aatatagaaa atagtctttg gcatgcccaa gtgcttcttg tgatatttgt gaacccgcta
     2641 atatgtttag tataggggtg ctaaaaagaa aggcaataat tatagtagca gctgccgcta
     2701 taagtgaaag cagaagaccg gaatcggcca ctcggcttaa ggtcttttcg tctttagcgc
     2761 ctattgaacg agctacaagc gagctaattc cgttaaaaat accgctgccc aaagcaatat
     2821 aaaggaagaa gagcggaaaa acaagtccaa gtccgctcat taatgtttca gagctacggt
     2881 caatataaga gataaatatt gtgtctgcta tattgtaaaa gaacataagc atatggccaa
     2941 taactacggg tacacttagt ttagtgataa tcttgtaaat atgaccttcc agtatctggt
     3001 tttgcacggt gctgcctccc tgtttttgag taaagccatg ctattttaaa gaatcgtttt
     3061 gtaaagcaaa aaaagtataa aataatatat taaaatttat ttaaagttaa atgctaaggt
     3121 taagggctgc tttccgataa tattgttatt taaagctata catttggtaa taatatgcct
     3181 gttatacgcg gtgacactgt tttaaggtga agttaagttt ttttgttgtc ttaaaacaga
     3241 taaccattat tatttgacat tcattcaaaa tcagttttga catgtattgg aaacggttgc
     3301 gtaatccgga ttgtaatttg taccgattgc tttgtgtgtt ttccgggatg ctttaatgga
     3361 atttgactcg aaaaccctct gtctattaat acaaaatgat gccattatcc cgaaaaacat
     3421 cgatcgttac agtcaaactg gttttgtgaa acataataag agtatatttt gaaaactatc
     3481 agtttttcgg atatacaaaa cagtaattgc agattaatca aataatctgt aaaaccagac
     3541 tgtcggagtt tattcatgtc taaaaagtat tttattggta tggatcttgg ttcaactact
     3601 gccaaaattg ttgtcgggta cccaaatgat agcggtattt ctattgaata ttccgcttat
     3661 aagagacacc acgctaagat tcgggaaacc atagttggta tattgaaaga tgttcgcgaa
     3721 aagataggca acgcggagtg tcttgtctct attaccggat cagccgggat gggtatttct
     3781 gaaactacag gccttcgttt tgtgcaagag gttgtggctt cagcaaccta tattaaaagg
     3841 gtacagcctg acattagaac ctttattgaa ataggcggtg aagattcaaa aatcatattt
     3901 tttgacgatg attttcatgc cgatatgaga atgaatggaa gctgtgccgg aggaaccggt
     3961 tcttttatag atcagatatc tgtattgctg gacgttccag tcgaagagtt tgacgcttat
     4021 gcttcaaggg gtgtcaaggt tttccctatt gctacacgtt gcggtgtttt tgccaaaaca
     4081 gatgtccaga gccttctttc acataatgta tccaaagaag atattgcctt gtcgtctttt
     4141 cacgcggttg cgctgcaggt tcttagcaca ttaacccgtg gacacgatat cgagtcaaaa
     4201 atattgcttg gtggaggtcc cttgaccttc aataagtatt tgcgaaaggc ttttgttgag
     4261 tgtatcaagg atattcctga aaacgaattt gttgtaaccg ataaccctca gcttatacct
     4321 gcaattggta cagctcttac ttgtgacgat caaaacgaag ttcaatatct tgatgatctt
     4381 attactcttg ttgaaaaggt acccgagaag aaggtagaaa gtagcacgcg tactaccgcc
     4441 ataccggaac tttttaaaga cgatgatgat tataatactt ggcttaaaac ttttgagaag
     4501 taccatgtta accgtatgga ttttgatctt tacaaaacta aggactgctt tcttgggata
     4561 gattcaggat ctactacaac aaaaattgta ctttctgatg atgatggcaa tgtcgtcgca
     4621 acatattatc ataataatct gggtgaccct attggtactg tgcagaaggg ccttgccgaa
     4681 atatcaggtt tttgtaaaga gatgggattt acacctaata ttgttggaag cggggtaacg
     4741 ggctacggtg aaggtctaat aaaatgcgcg tttgatctag atcatggttt tgttgaaaca
     4801 atggctcact atcgtgcagc ttcacatttt gcaccagatg tctcttttat tctggatatc
     4861 ggtggtcagg atatgaaggc tatgtttata cgtgacggct atgtggcgga catacagata
     4921 aacgaagcgt gttcatcagg ttgtggttcg tttatcgaaa cgtttgctaa caacatgagt
     4981 tataaggttg ccgatttcgc ggaaattgcg tgtcgttcca agaatcctta cgacctgggt
     5041 acgcgttgta ccgtttttat gaatacaaag gtgaaacagg cacttaggga cggtgcttca
     5101 atcgaagata tttcagcggg actttcttat tcagtaatta aaaataccct ttataaggtt
     5161 cttaaactaa aagatagtga acctttgggt aaaaagatac ttgttcaggg cggaaccttt
     5221 aaaaacaatt cgatacttcg tgcttttgaa cagcttaccg gatgtgatct gatacggcca
     5281 gatattccag aacttatggg agcttttggt tgtgccttga aagcaatgga tctttataaa
     5341 gaaaatgctt atgatacaag ctttaagggt ttcgaagccg ctctgattat agcagatacc
     5401 gaaaagaaaa tcataaattg taaaggctgt gaaaaccatt gtcaggtaac aaaattaacc
     5461 tttgcgaatg gtaatcattt ttacataggc aacaactgtg aacgaatttt taccaacaac
     5521 accgaggttg ttaccagggg ggataatcta gtatcggaaa aaagggatat catttttaga
     5581 cgcaatcttt ccgcacgaaa accccgtggt ataaaaatgg gtattccccg aattttgaat
     5641 atgtttgaaa attttccgtt ttgggcaacc ttcttcaatg agtgtggcat agatgtagtt
     5701 ctttcggatc gttcgaatgt ggagatattt gaggctggtg tcaaatcggt tatgtccgaa
     5761 aacatctgct ttccggcgaa aattgctcac ggccatattc ttaatcttgt tgaaaaagat
     5821 gtagatagaa tatttttccc taatgttgtg tttgagcgag atgagtacga aggcgctcag
     5881 aatacctata attgccctgt tgttacagga tacccttgtg ttattcgaag ctccatagat
     5941 cctgaagggc gatacaatat tccttttgat tctccaccga ttagttttaa gagcagtcgg
     6001 cttattgtac gacagcttat aaagtacatg aaaactctca atgtgcggga aggcattgtt
     6061 aaacgtgcgt acaaggctgc aattcacgag cagaaggcca caaaaaaaag gctgcaagac
     6121 cgcgcacgtg acctgatcgc caaagccgac tcaactggac agcctttgct tgttatggcg
     6181 gggcgtccat accatatcga caaacttata aaccatggta ttccagagtt aataagtgat
     6241 cttggagcta tcgttattcc tgaagattca atacccgacc ttaatagcat aattatctcg
     6301 gatgtaaatg tgctttctca gtgggcttat acaaacagat tgtacgcaac cgctaaatgg
     6361 gtaagccagc atccaactgc tcaaatagtt caggtcaatt cgtttggttg cggtcctgat
     6421 gccgtatctg tagacgaggt aaaagagata cttgaagaga atgataagct ctatacaatt
     6481 attaaagttg acgaggtctt caacttaggt gctgtcaaga ttcggttgag atccatgctt
     6541 gaagcactca agaagaaaca gaaagtggtc aaagacggat cgatttctcg tcgtaacgtt
     6601 aatcaggcag tacctttttc aaaagagaag acggtaattg tccctcattt ttctaaatac
     6661 tattctcctc ttattccccc tctatttagg agctttggaa taaagatgga gacacttcct
     6721 cttcaggatc atgactctgt ggattatggt attaaaaaca taaacaatga gatatgttac
     6781 ccagcgattc ttgtggcagg tgatattata aaggcttata tggagaacaa ctatagtaag
     6841 gacgatacca tagttctttt gacgcagaca ggcggtcagt gtagagcttc caactatatt
     6901 aatctagtta acaaggcctt ggatcgtgtg gggtttggcg atgtacaggt tgctactctt
     6961 tctacagctt ctctaaaaac tgttgtaaca ggtcgggaag gccttctttt ggccaaaaaa
     7021 atggttttgg gaattctgct tcttgaccag ttgatgtaca tgtatttaac aaccaagccg
     7081 agagagataa aaaaaggaac atccaatgct ttacataaaa agtatatgga attttttaac
     7141 gagattattg ttaccaagca ttacgaaaaa agcatggata ttcttgctca ggccgtcgaa
     7201 gaatttaacg ctgttgaagt aagcaatgat ccggttccac gtatcggttt tgttggagaa
     7261 atttttgtaa agtataatga atttgcccat atggatctta tagaatgggt attggagcag
     7321 ggagcagaga ttgttcttcc tcctcttcaa ggcttctttt tgcatgattt tattagtcgt
     7381 gattatatgc acgacatgca tatagcaggt tcggttttaa gccgtctgat taatcgtttt
     7441 atctggaact atgtaaatgg taaggttaaa aaagtagatg ctattatgaa gagctacaaa
     7501 cgttataaaa agccacatga tcttcacaaa cttgctgata aaaccgagaa ttttgtgtcg
     7561 ctggtcaatc aattcggtga gggatggcta ttaaccgcag atatgctctc catgcttgac
     7621 gaaggtgtcg aaaatattat ttctgtgcag ccgttcggtt gtatatccaa ccacgttgtg
     7681 gctaaggggg tcgagaaaag gattaagaat ctttacccca atttcaaatt attggcaata
     7741 gattttgacc agagcactag tccggccaac gtttttaaca gagcgcattt tatggttgct
     7801 tcggctaagg aatctttgca aaaacagcaa gaaaaggcga ctggtacaga gtaatcggga
     7861 gaaaaattga gtattgataa cagagccggt ttaacccggc tttgtcgttt taaaaagacc
     7921 tttttaattt ttcgggggtc aatattcttg actaaaacgg gtactgctcc ataatcgctc
     7981 aaagtatatc gtgtagtcct attatgggga aaataatgag cgaaaattct gtgtttggcc
     8041 ggctttttga tgtaataagt gccagaaaaa aagaatctcc cgatcactca tatgttgcgc
     8101 gtcttatatt caagggtaca gagaagataa acagcaagat tatggaagag gctgccgaag
     8161 ttacagaagc agcactagat aatgatcgtt cccatctggt ttatgaaata tgtgatctgc
     8221 ttttccatac ttttgtattg gccggcagca aagatatttc gcttagggat atcgaaacag
     8281 aacttgagcg ccgttttgga aagagtggac tacaggaaaa ggcggaaagg aaaaacaatg
     8341 cttaatgatg aaagcagagt tagatttgcc ctaccgggta aaggcaggct taaggatccg
     8401 gctattgaac ttttgcagaa ggcaggattc aggtttcgcg ctaaggggcg aaatctttat
     8461 gctacctgta cttcagataa tataacattt atatttgtgc gcgccgacga tattcctctt
     8521 cttgtacaga aaggtgttgt tgacatggga ataaccggta gcgatctagt tattgagcgt
     8581 gatgctgatg ttaagcaact tcttgagctt ggtttcggtc gctgcaggct gtgcgtagct
     8641 gttaaagaag agtgtgattc taaagattac agctttttga agaatgctac tgtagcaaca
     8701 tcttttccaa gaataaccac agatttcttt aaaaaaaaca ataccgatat aaatattgtt
     8761 cagatgaacg ggtcagtcga aataatggta gcgcttggtc ttgccgatgc tattgttgat
     8821 atagttgaaa ctggggactc tttaagagat aataatctta aggtattttc ggatatagca
     8881 agttatgaaa cagttttgat tgcaaatcat ggttttgctt ccaaatcgcc aatgaccgaa
     8941 atgataagta ggcgtattga aggtatactt gtggctactc gttacagtat attggaatac
     9001 aacattgaca aaaaaaatct tttcaaggcc gagaagatta caccaggcta taatagcccc
     9061 actctttcac cattagagaa tgaagggcta tttgctgtca aggttatggt tctaaaaggt
     9121 gatgttccat cggttatgga taaacttgag gccattggcg caacggcaat aattgagtca
     9181 gaaattaaaa actgccgttt atagattttt accggttaag ccggaaatga caataagacg
     9241 gtgaacctat gctgattgct aaaggttccc ttactacaga ttatcaggag aaatattatg
     9301 ctttggggtg ttaaaatcga agtgcttgcg gtttatgcgg gtattgttgt tctgatcgcg
     9361 gcggttgttg ttgttatcaa gatgttaaga aaacgttttg cgaaaaaagc ggacgagcaa
     9421 aaggcaattg ttaacgaaca caaaatgcca gcttcaatat ttgtacttga gaaaaaaaag
     9481 ggcaagatta cagatgccaa attgccaaag aatgtattgg atcaggttcc ggcaatatac
     9541 aaactaaaaa agatgcctct tgttgtagcc aaggtgggtc cacagatagt cacacttata
     9601 tgtgaagaga atgttttttc ccagattcct gagcataaaa acgtaaacgt tgaagttgct
     9661 ggaattttca ttgtgtcagt taaacaccaa tcaaataaag gaaaagcaga atctaaacgt
     9721 aaacg
//
LOCUS       DAOTGZ010000092         9661 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_3064
            NODE_8395_length_9661_cov_4.446804, whole genome shotgun sequence.
ACCESSION   DAOTGZ010000092 DAOTGZ010000000
VERSION     DAOTGZ010000092.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868088
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 9661)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9661)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:22:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,785
            CDSs (total)                      :: 2,751
            Genes (coding)                    :: 2,732
            CDSs (with protein)               :: 2,732
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 12 of 19
            Pseudo Genes (internal stop)      :: 5 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9661
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8395_length_9661_cov_4.446804"
                     /isolate="AMR_MDS_3064"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..308)
                     /locus_tag="P5123_07430"
     CDS             complement(<1..308)
                     /locus_tag="P5123_07430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012198294.1"
                     /note="Members of this family of hydrolases with an active
                     site Cys residue belong to MEROPS family C26; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamyl-gamma-aminobutyrate hydrolase
                     family protein"
                     /protein_id="HRX16128.1"
                     /translation="MIAILDYGSQYSQLIARRIRECNVYCELLSHDLDLEKLKEKNLE
                     GIILSGGPSSVYEEGAPSMDKAILESGVPVLGICYGMQLIVHLLGGKVEQESEKREYG
                     T"
     gene            complement(305..1774)
                     /gene="guaB"
                     /locus_tag="P5123_07435"
     CDS             complement(305..1774)
                     /gene="guaB"
                     /locus_tag="P5123_07435"
                     /EC_number="1.1.1.205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018248322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003938 - IMP dehydrogenase activity
                     [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IMP dehydrogenase"
                     /protein_id="HRX16129.1"
                     /translation="MQNKFSKTGLTFDDVLLIPAKSEVLPDKVSLTTRLTDKISLNIP
                     IISAGMDTVTESPMAIAMAREGGIGVIHKNMSIEDQSAEVNKVKRSENGVIVDPFFLK
                     PDNTVQEAVNLMNKHHISGVPITDENGKLTGIVTNRDVRFESNYDQPIKNVMTSKNLI
                     TAPEGTSLEGAREILKNTKFEKLPLIDKDGRLKGLVTIKDIQKTLEHPHAAKDEKGRL
                     LTGAAVGISADADDRIAALVEKDVDIIVIDTAHGHQINVINAVKRIRQKYPDLQIIAG
                     NVATPEATRDLIKAGVNAVKIGIGPGSICTTRVVAGIGVPQITAIYECALAADEFNIP
                     VIGDGGIKFSGDIVKAIGAGASVIMIGSLLAGCDESPGSLELLHGRRFKTYRGMGSEE
                     AMKAGSKDRYFQNNTNKFVPEGVEGWVPYKGGVNEVIFQLVGGLRAGMGYCGTGSIED
                     LRKNANFIQITGAGLRESHPHDIRITKEAPNYTTDGLFE"
     gene            2209..2490
                     /locus_tag="P5123_07440"
     CDS             2209..2490
                     /locus_tag="P5123_07440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015902978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease signature motif containing
                     protein"
                     /protein_id="HRX16130.1"
                     /translation="MLPVNIEEEKQKARKLRNSPWWKRKISDGVCHYCREHFKPSELT
                     MDHLIPLARGGRSEKINLVPCCKECNNKKKYMMPFEWDEYLKQLSEIKS"
     gene            2564..4996
                     /locus_tag="P5123_07445"
     CDS             2564..4996
                     /locus_tag="P5123_07445"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRX16131.1"
                     /translation="MASKQEKFSAGGLISLQGKNQGCFCSLMSGRVEMLSAPSDYSGL
                     DQSLVLKHSVRVGIFESKNHFFILPGSTYRAVTDCVIQKSNMTPQSFATVAASDPGTA
                     ITALSYQFANTESTYKEMVRVSKVKKNLEQISDNVMILFRELSPRDVSGELQSHIDNL
                     YTKMSQSEAQLPAEITSSVLMNDNSRFLQKNYQEGSLEFGSFCDKSHYDFYKRFLKIN
                     TNVLAHLVKGDPAIPGFMFAKVFDIYCKLSSRLMSMQLEIEKHMKLLFASDSSMSSTL
                     IHEGAINDFIASGRLGRTFLTDFVSLVQKIYSMYKDVSGKDASALFPGLAEISGFVAT
                     NRQPGATPAQGSGDSAVSFSEGSSQVLSLYNNSLQQIFEFAVAEAEFKKEFLHHLNHF
                     KKIENPFSPDSDARKVRRHLTRMYWQLYNKVYIRSKRSKSVPAPAKLMLLYGFIDENL
                     AEPQQIPVLHRLALDRYKPTAPIMYEYEFLSRIYAGKETPSVSEMGLTFEKQLREMAK
                     GTRKGKNPMEYLDDPIYKTQYEIDNMLQSTTSVCSGSRSTAFPIFTEKLLRGDPQSFV
                     VTKSKLDAIFKELVDYDYSCFYRETVYKGADTRDVIEEEVYPYIILVPTFGTKTMMWQ
                     EIVDINKRSRARIVVPVHFIGDLKTELAHAFAIYRWELNRTLKGGLWADPVEGGITGA
                     YSDYIQFYKKNKKLSDEARKKIAEKIRSVRGNTREIFAEDYIMWLTYEKDGVMKLNPV
                     VRDMFYRYIPFKNEIREKLERMPAFQDSAIRFKNIRNRTFAAFERKFRKYKDENENYP
                     PAIEKYLKYLQM"
     gene            5007..5825
                     /locus_tag="P5123_07450"
     CDS             5007..5825
                     /locus_tag="P5123_07450"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRX16132.1"
                     /translation="MARRKVSTVINIYSKKVKDQIVELCRNFFGREGLEYLVGDVFQC
                     VDELIKNSIKANYKFIMVLSKLYDKHMLDQPDADEKTLNEQINNLIKDKSSFDIIAPE
                     IYREHNLVDQVRRVLNEEATQIKLKDKAYISNRECNASEIEQLRSLKRLAHIKNLLKK
                     NDVRTLLKIESDGQFIFIEVTNTAPITDIDLKRIHEKRDEFLQYHKVGREYEFFVNHL
                     DTTDSGFGLGYATIDSMLIGIGLDPYQTIQILAASDTTVILSFPIAELKQLQKD"
     gene            5853..6512
                     /locus_tag="P5123_07455"
     CDS             5853..6512
                     /locus_tag="P5123_07455"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRX16133.1"
                     /translation="MKLLIFISVISVFSCGTATTGGNTKSIIISDPEPPEDKYSVSNY
                     VAEKPDEYARFLVKPEDYTTDGEYYHKKHERCLLGLAEVLTEKHRYNIKERSIGFYYD
                     RLGKETDLLYLGFDVSCSQNFVDEDKNYFINGTAMIDKYLPNALEVTRRYDDILSETS
                     VEGVVVGLFWFRRGRRELMTVWLLKQNIREFYDDHLTFRQLLIKSTVTDSDGKKIRLA
                     L"
     gene            complement(6520..7587)
                     /locus_tag="P5123_07460"
     CDS             complement(6520..7587)
                     /locus_tag="P5123_07460"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0052621 - diguanylate cyclase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sensor domain-containing diguanylate cyclase"
                     /protein_id="HRX16134.1"
                     /translation="MTNKNEQLQKLKDENNYLKEYTLKLSTLIDVNSLVVSSLDKETV
                     LKTILEQTKILMSCSEASVLLVDHNKRSLYFALFSETNNAEPLAAIRIKRGEGIAGKV
                     WQTGEPVITSEKGYDNRVSRKVDQKLDSKTHSLIAVPLTVKGEIIGVMEAINKNDTGA
                     FTRDDLEIFQILSNQAAIAIYNADLYEMATRDGMTKLYIRRYFDTRLAEEFSRSSRYN
                     RDIALIMFDIDHFKSFNDNYGHQLGDKVLIKTAEIIQNFCRACDIPSRFGGEEFSVIL
                     PETGIQGAENLAERIRAGVESETIYDNNQKIKLTISAGIATRQTSGSVSAKKLVSNAD
                     KALYMSKQNGRNMVTLFQKTE"
     gene            complement(7691..>9661)
                     /locus_tag="P5123_07465"
     CDS             complement(7691..>9661)
                     /locus_tag="P5123_07465"
                     /inference="COORDINATES: protein motif:HMM:NF019315.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate/guanylate cyclase domain-containing
                     protein"
                     /protein_id="HRX16135.1"
                     /translation="SLEGEWEFYWMTLTEPGKSVSDYTHNMRYYDIPGTWNSDISIYP
                     PHGYATLRLTVLLPDHDQVYALYIPPMLTAYSLWIDGKIISFNGKIGINGRDSSPQYM
                     TKIAPFKPESDKIEIVCQISNYHYRKGGMWSPILIGHANRINRLRDLNVAIALFLCGA
                     FIIMALYHIGIYIFRPQELSSLFFALFCLTFTIRIITTGEILLSEFITDINWGLLIKL
                     ELMPLFFGGPVFLQFFKTLFPKDVGNIIRKTIIYSALAIGAFIIILPVSYASYLVPPL
                     EVIIVLGMLYITICLIKALRLNRPGAKYMFFAFIIFFATAINDILFALEFIQTSYMLP
                     FGLFIFILAESFLLAKNIARSYTNVELLSGELHKTNRAMSRFVPNEFLAFLNKKSIMD
                     IRLGDQHLQFMTILFCDIRSFTTLSEKMSPQQNFNFLNSYLKKIGPVIRKNNGFIDKY
                     IGDEIMALFPNSASDAVKTAVEIFTEIKSFNITSVSKGFPPINVGVGIHTGNIMLGII
                     GEDQRIESTVISDTVNLASRIEKLTRLYKTDILVTGKTLTKLDNLGRLHVRMIDIVQV
                     RGKTEESAIFEIYDSNDYDTIAIKDKTKEELEYAINLYFKADFIKSLELLEKILNQNP
                     NDGVVNLYIKRCKKYIELGTPANWTGVSRFEG"
BASE COUNT         2811 a         1761 c         2044 g         3045 t
ORIGIN      
        1 gtgccatatt cccgtttttc actctcttgt tcaacctttc cgcccaaaag atgcacaata
       61 agctgcattc catagcatat tcctaaaaca ggtacgccgc tttccagaat tgctttatcc
      121 atggaggggg ctccctcttc gtacactgaa gagggaccgc cggaaagtat tatgccttcc
      181 agatttttct ctttaagttt ttcaaggtca aggtcgtgcg atagaagctc gcagtaaaca
      241 ttacactcgc gtatgcggcg agcgattaat tgtgaatatt gagatccgta atccagtatt
      301 gctatcattc aaataacccg tctgtagtat agtttggtgc ttcttttgtt atacgtatat
      361 catgtggatg cgactcgcgt aagccagctc ctgttatctg tataaaattg gcattttttc
      421 ttaaatcttc tattgaacct gttccgcaat accccattcc ggcacgaaga cccccaacaa
      481 gttgaaatat gacttcgtta accccacctt tatagggaac ccatccttct acaccttcag
      541 gtacaaattt gtttgtattg ttttgaaaat agcggtcttt ggatccggct ttcatcgctt
      601 cttctgaccc cattcctcga taagttttaa atcttcttcc atgaagaagt tccagtgatc
      661 ccggtgactc gtcgcaaccg gcgagaagag agcctatcat tatcaccgaa gctccggctc
      721 cgattgcttt tacaatatct ccggaaaact taataccacc atcacctatt accggtatgt
      781 taaactcatc tgctgccaga gcacattcgt atatagctgt tatttgagga acaccgattc
      841 cggcaaccac gcgtgttgta cagattgaac caggaccgat tccaatcttt actgcgttaa
      901 caccagcttt gataagatct cttgtcgctt ctggggttgc aacatttcca gcaataatct
      961 gaagatcagg atacttttga cgtatgcgtt ttacagcatt gataacgttt atctggtgtc
     1021 catgtgcggt atcaataaca ataatgtcta catccttctc gacgagggca gcaattcggt
     1081 cgtcagcatc cgcgctgata ccaaccgctg ctccggttaa aagacgaccc ttttcgtctt
     1141 tggcggcgtg agggtgctct agggtttttt gaatatcttt aatggttaca agtcctttaa
     1201 gtcgaccatc tttgtctata agaggtagtt tctcgaattt tgtgtttttc aatatttcac
     1261 gtgcaccttc gagtgaagtt ccttcgggag cggtaatcag gttttttgat gtcataacat
     1321 ttttaatggg ttgatcgtag ttagactcaa atcgaacatc tcggtttgtt acaatacctg
     1381 tgagtttgcc attctcatct gttataggaa cacccgaaat atgatgtttg ttcattaaat
     1441 ttacggcttc ctggacagta ttgtcgggct ttagaaaaaa aggatcaaca ataactccat
     1501 tttcagaacg cttgaccttg tttacttctg cggattggtc ttcgatactc atattcttgt
     1561 gaattactcc gatgccgcct tctcgtgcca tggcaatagc cataggtgat tcagtaactg
     1621 tgtccatgcc agcgctaatt attggaatat tcagcgatat tttatcagta agtcttgttg
     1681 tcagagaaac cttgtctgga agaacttcgg atttcgccgg tatcagaagc acatcgtcga
     1741 aagtgaggcc ggtttttgaa aatttatttt gcattttatt aaaacctcat ggagggtgta
     1801 ggataaagta cagtatcaaa aaacggacaa tttctatcaa tacatttttg gaaaccattt
     1861 gcctaactgg tatctcttgg tattttcgag tgatatggct ctaaatgaac cgccgttgtt
     1921 ttgcctaatg tagtgagagg gtagagctta ggcaataaaa aaattatcta taacatcact
     1981 tgacctatga cttcttttaa aataatgtaa tttttgatat tctttatata aaccgcgata
     2041 atttcaaatt atcgcatgag ttatttacaa tctgcacaga ctaatatgta ttgacagaaa
     2101 atagaccttg attataatat ttaatggaac gatatgggaa gacgtgataa taaaaaaaac
     2161 agatattcaa atggttttcg gtctgatgaa cctttgcatt tctccgagat gctgcctgta
     2221 aatatcgaag aagagaagca gaaagccaga aaactgcgca acagtccgtg gtggaagcgt
     2281 aaaatatctg atggtgtttg tcattattgt cgtgagcact ttaagccctc agagttgaca
     2341 atggatcatc tgattccctt ggcgcgtggg ggcagatctg agaaaatcaa tcttgttcca
     2401 tgttgtaaag agtgtaataa taaaaaaaag tatatgatgc ccttcgaatg ggatgaatat
     2461 ttaaagcagt tatctgaaat aaagagttaa attgcagggt gaaattcttt tatattggcg
     2521 tgattttatg tgtcctgtta ttattgttta aggagtttct tttatggcta gcaagcagga
     2581 aaagttctct gcggggggcc ttatctctct tcaaggaaaa aatcagggtt gtttctgttc
     2641 cctgatgtct ggcagagtag agatgctttc tgctccgagt gattattcgg ggctggatca
     2701 gtctttggtt cttaagcata gtgtgcgcgt tggtatattt gaatcaaaaa atcatttttt
     2761 tatcttgcct ggatcgacct atagagctgt gaccgattgt gttattcaaa agagtaatat
     2821 gacacctcaa agttttgcta cggtggctgc ttctgatcct ggaactgcta ttacggcact
     2881 ctcttaccag tttgcaaata ccgaatcgac ctacaaagag atggttcgtg tttcaaaggt
     2941 taaaaaaaat cttgagcaga tatcagataa tgtaatgatt ctttttcgtg agttatcacc
     3001 cagagatgta tcaggagagc tgcagtccca tattgataat ctttatacta aaatgtctca
     3061 gtccgaggca caattgccgg ccgaaattac ctcttcggtt ttaatgaacg ataacagccg
     3121 ctttcttcaa aaaaactatc aagagggttc tctggaattc ggctcctttt gtgataagag
     3181 ccattacgat ttttataaaa gatttcttaa aataaacaca aatgtactgg ctcatcttgt
     3241 caagggagat cccgctatac caggatttat gttcgctaag gtttttgata tctattgtaa
     3301 gcttagttcc cgtttaatgt ctatgcagct tgaaatagaa aaacatatga aactgctttt
     3361 tgcttctgat tcaagcatgt cttcaaccct aatccatgaa ggggcaataa atgattttat
     3421 agcaagtgga agattggggc gtacctttct gacagatttt gtatcgcttg tgcaaaagat
     3481 ctattccatg tataaagatg tttcgggcaa ggacgcttca gcgctttttc caggtttggc
     3541 tgaaatatct ggttttgttg ctacaaatag gcagccagga gcaacgcctg cacagggaag
     3601 tggtgattcc gctgtttcct tttccgaagg ttcttcgcag gtcttatctc tttataataa
     3661 ttctttacag cagatttttg aatttgctgt tgccgaggca gaatttaaga aggagtttct
     3721 tcaccatctt aaccatttta agaaaataga aaatcctttt tcgcctgatt ctgacgctcg
     3781 aaaagtgagg cgtcacctta cgcgtatgta ttggcaattg tataataagg tctatattcg
     3841 tagcaaacga tctaaatcag ttccggctcc tgcaaaactt atgcttcttt acggctttat
     3901 agatgagaat cttgcagagc cccagcagat accggttttg cacagactag cgcttgatag
     3961 atataagcca acagcaccaa taatgtatga gtatgaattt ctttcccgaa tttatgctgg
     4021 gaaagagact ccgtctgttt ccgagatggg actgactttt gaaaaacagt tgcgagagat
     4081 ggcaaagggt actcgaaaag gtaaaaatcc catggagtat ctggatgacc ctatctataa
     4141 gacacagtac gaaattgata acatgcttca gtcaacaacg agtgtctgct ctggctcacg
     4201 atcaacggct tttccgattt ttacagaaaa gcttttgcgg ggggatccgc agtcttttgt
     4261 tgtaacaaaa tcaaagcttg atgctatttt caaagaattg gtagactacg actactcatg
     4321 cttttaccga gagacagtgt ataagggcgc ggacacaagg gatgttattg aagaagaggt
     4381 ttatccctat attattcttg ttccgacatt tggtacaaag accatgatgt ggcaggaaat
     4441 tgtggatatc aataaacgca gccgcgcgcg tatagttgtt cccgttcatt ttataggaga
     4501 cttaaaaaca gaacttgctc atgcgtttgc tatatataga tgggagttaa accgcacact
     4561 taagggcggg ctttgggctg atcctgttga aggtggaata accggagcgt actctgatta
     4621 catacagttt tataaaaaga acaagaagct gtctgatgaa gcgcgtaaaa aaattgccga
     4681 gaaaattcgc tcggtcagag gtaatacccg agaaatattt gccgaagatt atattatgtg
     4741 gctcacgtat gaaaaagatg gtgtaatgaa gcttaatcct gttgttaggg acatgtttta
     4801 cagatatatt ccgtttaaaa atgaaatacg ggaaaaactc gagcgcatgc cagcttttca
     4861 ggatagcgcg ataaggttta agaatataag aaacagaact tttgcggctt ttgagcgaaa
     4921 atttagaaaa tacaaggatg aaaacgaaaa ttatccgcca gctattgaaa aatatctaaa
     4981 atatctgcaa atgtaacggt gagtaaatgg caagacgaaa agtatctaca gtaataaata
     5041 tttacagcaa aaaagtaaaa gatcagattg ttgagttgtg cagaaacttt tttggcagag
     5101 aggggttgga atatctggta ggagatgttt tccagtgtgt tgacgaattg ataaaaaact
     5161 caataaaagc caattacaag tttatcatgg ttttaagcaa actctatgac aagcatatgc
     5221 tggatcaacc tgacgccgat gaaaaaacat taaatgagca aataaataac ctaattaaag
     5281 acaaatcctc ttttgatatt atagcaccgg aaatttacag ggagcataat cttgttgatc
     5341 aggttcgtag ggttcttaac gaagaggcta ctcaaataaa actgaaagac aaagcctata
     5401 tttcaaaccg ggagtgcaac gcatccgaaa ttgagcagct tcgatcatta aaacgattgg
     5461 cgcatattaa aaaccttttg aaaaaaaatg atgtaagaac acttttgaaa attgaaagtg
     5521 atggacaatt tattttcata gaggttacca acactgctcc cataacggat attgacctta
     5581 aaagaattca tgaaaaacgg gatgagtttt tgcagtatca caaggttggt cgtgaatatg
     5641 aattttttgt taatcacctt gacacaactg attccggatt tggtctggga tatgccacta
     5701 ttgatagcat gttgatagga ataggtctgg atccttacca aaccattcag atattggcgg
     5761 cttccgatac gactgttata ctctctttcc cgatagccga attaaaacaa ttgcaaaagg
     5821 attaataggt gaaaattatt ttgagtcatt atgtgaaatt acttatattt atttctgtta
     5881 ttagtgtttt ttcttgtggt actgctacaa ccggtggcaa cacgaaatcc attattatct
     5941 cggatcctga accaccagag gataaatatt cggtgtctaa ttatgtggcc gaaaaacccg
     6001 atgaatatgc tcggtttctg gtgaaacctg aggattatac tactgatggt gaatactatc
     6061 ataagaaaca tgaacgttgt cttttgggac ttgctgaagt tttaacagaa aagcatagat
     6121 acaatattaa agaaagatct atcggttttt attatgatcg tcttggtaaa gagactgatc
     6181 ttttgtatct tgggtttgac gttagctgta gtcaaaattt tgttgatgaa gacaagaatt
     6241 attttattaa tggtactgcc atgatcgata agtatctccc aaatgctctt gaggtaacaa
     6301 ggcggtatga tgatattctt tcggaaacga gtgttgaagg tgttgttgtg gggctttttt
     6361 ggttcaggcg tgggcgtcgg gaacttatga cggtctggct tctaaaacag aatatccgtg
     6421 aattttatga tgatcatcta acattcagac agctacttat aaaatctaca gttactgact
     6481 cggatgggaa aaaaatacgt cttgccttgt gactaaatgt tattctgttt tctggaatag
     6541 ggttaccatg ttgcgtccat tctgttttga catgtaaagg gctttgtcgg catttgagac
     6601 aagttttttg gcactaacag agccgcttgt ttgcctagtg gcgataccgg ccgaaattgt
     6661 tagttttatc ttttgattat tatcataaat tgtttctgat tcgacacctg ccctgattcg
     6721 ttcagccaga ttctcggctc cctgtattcc cgtttcggga agaatgacag aaaattcttc
     6781 accaccgaaa cgtgaaggga tatcacaagc gcgacaaaag ttttgaatta tttcagctgt
     6841 tttaataagt accttatccc ctaactggtg accgtagttg tcgttaaaac ttttaaaatg
     6901 atcaatatca aacattatta gtgctatgtc gcggttatac cttgatgatc tggagaactc
     6961 ttcggctaat ctggtatcaa aatagcgtcg aatatagagt tttgtcatac cgtcacgtgt
     7021 ggccatttcg taaaggtcgg cattatatat agcgattgct gcctgatttg aaagaatctg
     7081 aaagatttcg agatcgtccc tggtgaaagc tccggtatca tttttgttga tagcttccat
     7141 cactccgata atttcacctt taacggtaag aggaactgca atcaaagagt gtgttttgct
     7201 atccagcttt tgatcgacct tgcgtgaaac acggttatca taaccttttt cggaagttat
     7261 caccggttcg ccagtttgcc agactttacc ggctattcct tctccacgct tgattcgaat
     7321 agcagcaagg ggttctgcgt tatttgtctc agagaacagg gcaaagtata gagatcgttt
     7381 attgtgatct accagcaaaa cacttgcttc gctgcagctc attagaattt tggtctgttc
     7441 tagtatcgtt tttaatacgg tctctttgtc cagtgatgat acaacgagtg aatttacatc
     7501 aatcagggta gaaagtttga gggtgtattc tttcaggtag ttgttttcat cttttaactt
     7561 ttgtagctgt tcgtttttat ttgtcattta acagtaccga gattacctgc gtataaaatt
     7621 attctgtaga taatttaact ataaaatgga ataaaacatc taaaaacgat atttttataa
     7681 aatactaggc tcaaccttca aatcggctta cgccagtcca gttggcaggt gttcccaatt
     7741 cgatatattt tttgcacctt ttgatataaa gattaacaac tccgtcgtta gggttttgat
     7801 tcaggatttt ttcgagtagc tctagtgact ttataaagtc agctttgaag tagagattaa
     7861 tcgcgtactc taactcctct tttgtcttgt ctttgatggc aatagtgtca taatcattgc
     7921 tgtcgtaaat ttcaaaaata gcggactctt ctgttttgcc tctcacctgt acgatatcta
     7981 tcattcgtac atggagcctg cctaagttat caagttttgt taatgtttta cctgtaacta
     8041 gtatatctgt tttgtatagc cttgtcagct tttcaattcg cgaagcaagg tttactgtat
     8101 cagaaattac agtgctctca atgcgttggt cttcaccgat tatacctaac ataatattgc
     8161 ctgtatggat gcccacaccg acgttgatag gagggaagcc cttggaaacg gatgtaatgt
     8221 taaaggattt aatctctgta aagatttcga cagctgtttt aaccgcatcg gaggctgaat
     8281 tggggaatag agccattatc tcgtcgccaa tgtatttatc aataaaaccg ttgtttttgc
     8341 ggataacggg gccgattttt ttaagataag agttcaaaaa gttgaagttt tgttggggac
     8401 tcattttttc ggatagggta gtaaaggagc gtatatcaca aaaaagtatg gtcatgaatt
     8461 gaagatgctg atctcccaga cggatatcca taatgctttt tttgttaaga aaagccagga
     8521 attcgttagg aacaaaccgg ctcatcgccc tgtttgtctt gtgcagctct ccggatagca
     8581 gttcgacatt ggtgtaagat ctggcgatgt tctttgcaag aagaaaagat tctgcaagta
     8641 tgaaaataaa aagaccaaat ggaagcatgt aagaagtttg aatgaactct agagcgaaaa
     8701 gaatatcgtt tattgctgta gcgaaaaaga ttataaaggc aaaaaacatg tatttggcac
     8761 caggacgatt taaccgtagt gcttttatca gacaaattgt gatatatagc atgccaagca
     8821 ctatgattac ttcaaggggg gggaccagat agcttgcata cgagacgggc agtattatta
     8881 tgaaggcgcc aatcgccaaa gccgaataga taatagtttt tctaataata ttccctacgt
     8941 ccttcgggaa aagtgttttg aaaaactgaa gaaatactgg gccgccgaaa aagagtggca
     9001 taagctcgag ttttataaga agtccccaat taatatcggt gataaattca gataagagta
     9061 tttcgccggt ggttatgatg cgaattgtaa aagttagaca aaatagtgca aaaaagagcg
     9121 aggagagctc ctggggtctg aaaatatata ttccaatatg gtagagtgcc ataattataa
     9181 aagctccgca aagaaaaagg gcgatagcta catttaaatc tcgcaaacgg ttaattctgt
     9241 tggcatgtcc tatcagtatt gggctccaca tgccgccttt gcggtagtgg tagttggata
     9301 tttgacatac tatttctatt ttgtctgatt caggtttaaa aggggctatt ttggtcatgt
     9361 actgtggaga agagtcgcgg ccattgatcc caatttttcc gttaaaggag attattttgc
     9421 catcgatcca gagagaatat gcagtaagca ttggcggtat atatagggcg tatacctggt
     9481 cgtgatcggg caagagtaca gtaagtcgca aagtagcata gccatgaggg ggataaatgc
     9541 ttatgtccga gttccaggtt ccggggatat cgtagtagcg catattatgt gtgtagtctg
     9601 agacgctctt tcctggttca gtcagagtca tccagtagaa ttcccactct ccctcaaggc
     9661 t
//
LOCUS       DAOTGZ010000093         9607 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_3064
            NODE_8449_length_9607_cov_4.309464, whole genome shotgun sequence.
ACCESSION   DAOTGZ010000093 DAOTGZ010000000
VERSION     DAOTGZ010000093.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868088
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 9607)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9607)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:22:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,785
            CDSs (total)                      :: 2,751
            Genes (coding)                    :: 2,732
            CDSs (with protein)               :: 2,732
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 12 of 19
            Pseudo Genes (internal stop)      :: 5 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9607
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8449_length_9607_cov_4.309464"
                     /isolate="AMR_MDS_3064"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1636
                     /gene="speA"
                     /locus_tag="P5123_07470"
     CDS             <1..1636
                     /gene="speA"
                     /locus_tag="P5123_07470"
                     /EC_number="4.1.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005988606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008792 - arginine decarboxylase activity
                     [Evidence IEA];
                     GO_process: GO:0006527 - arginine catabolic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="biosynthetic arginine decarboxylase"
                     /protein_id="HRX16136.1"
                     /translation="RYQGVYPIKVNQQKQVLKEVTRFGERYHHGLEVGSKPELIAALS
                     VMKDKEACLVCNGYKDEEFIDLGLYATKMGFKCFFVVEIPGEVDMILQRSRKLGITPN
                     IGVRIKLASRAGGHWTESGGDRSIFGLNVSQLIDVVDLLKKEDMLHALKLVHYHLGSQ
                     IPNIRDIRSAVVEAARVYAELVKEGAPMGYLDLGGGLAVDYDGSKTNYSSSRNYSLSE
                     YCADIIEVVMSIMNEHEIEHPTIITESGRAIVAYYSVLLFNVLDTSTFEHPVVPDTLD
                     PETHEQIINLHDVYKGLTLKNLQESFNDAIYYRDIIRDHFKHGQLSLRERSLAESIFW
                     NIITYIAAEKSRVKRVPQELDGIGDAIADIYYCNFSVFQSVPDSWAIDQLFPIMPIHR
                     LEEMPTRKAVIADITCDCDGKIDRFIDTHDVGHTLRLHALNNGSDYYLGVFLVGAYQE
                     TLGDLHNLFGDTNVVSIQINKSGGFDIVREIEGDSVEDVLSYVEYDTKRMVEDFKQSA
                     EEAIREGRITAADRRNIINAYQNGLRGYTYYERFEE"
     gene            1640..2818
                     /locus_tag="P5123_07475"
     CDS             1640..2818
                     /locus_tag="P5123_07475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012662512.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="saccharopine dehydrogenase family protein"
                     /protein_id="HRX16137.1"
                     /translation="MGSVVIIGAGGVGQVVAHKCAQVPEVFEKITLASRTKSKCDAIA
                     AGIDRKIDTAQIDADDVPALTEFLLKIKPDLVINVALPYQDLHIMDACLAAGVHYLDT
                     ANYEPLDEAKFCYKWQWEYQERFEKAGLTALLGSGFDPGVTNVFTAYAAKHFFDEINE
                     LEIIDVNGGDHGQPFATNFNPEINIREVTAPCKHWEVDKFVETEAMSVKKSFECPENV
                     GTYNIYRMYHEEMESLVKHFPTIKKASFWMSFSDNYLKHLEVLGNVGMTRIDPVMYNG
                     QPVVPLQFLKAVLPDPSSLGPLTKGRTCIGCVCTGKKDGKELKKYIYNICSHEDAFKE
                     VKSQAISYTTGVPAMIGAMMMLTKKWLKPGVFNMEQFDPDPFMDALNKHGLPWKETTL
                     "
     gene            2797..3951
                     /gene="nspC"
                     /locus_tag="P5123_07480"
     CDS             2797..3951
                     /gene="nspC"
                     /locus_tag="P5123_07480"
                     /EC_number="4.1.1.96"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015774489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016831 - carboxy-lyase activity [Evidence
                     IEA];
                     GO_process: GO:0008216 - spermidine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carboxynorspermidine decarboxylase"
                     /protein_id="HRX16138.1"
                     /translation="MEGDNVIDAQTVQKYNQFQTPCFIVERGRLLKNCETLDTVKKRT
                     GAKILLALKGFAMYSVFDDIGDYLDGVCASSPHEARLGREEFKKEVHAFAAAYSQKDY
                     NDICQFANHIVFNSIDQYKRFELKDTIDYGLRINPRHSEIDVAIYDPCAPFSRLGIIS
                     DNLPDRLPEGITGLHMHTLCEKNSDAFSRTLMAAEEQFSEQFKQCSWINFGGGHHITR
                     QDYDIDLLCDTLNEFKTRYKAELYLEPGEAVALNTGFLTATVLDVVDNGMKIAIVDTS
                     AAAHMPDVLEMPYRPYIIGSGKPHEKAHSYRLGGLSCLAGDIIGDYSFDKPLAVGDKL
                     IFTDMAHYSMVKTNTFNGLTLPDIIYYDEANDKILHHKTFGYENFKNRLS"
     gene            3978..4670
                     /locus_tag="P5123_07485"
     CDS             3978..4670
                     /locus_tag="P5123_07485"
                     /inference="COORDINATES: protein motif:HMM:NF013232.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5'-methylthioadenosine/S-adenosylhomocysteine
                     nucleosidase"
                     /protein_id="HRX16139.1"
                     /translation="MKKIALIVAVKTEARELLADSFWGWEKKEPMHYCSSRFPVTLTI
                     SGIGKVFATYAMSQAANGADTVLSVGTSGGLGKQRVGDLYICHEFVEHDIDLTHLGIP
                     FGVTPYDAMDSPVISPETDKLLEERVIAAIDELGLTHNFGRSMSGDQFITDKEIIGHK
                     IETFSADLTDMESSACAKLAAYRIKIPFFALRYVSDNADHNASVSWTEEVKKAAVILN
                     DVLKLAVNSMDC"
     gene            complement(4701..5111)
                     /locus_tag="P5123_07490"
     CDS             complement(4701..5111)
                     /locus_tag="P5123_07490"
                     /inference="COORDINATES: protein motif:HMM:TIGR01784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rpn family recombination-promoting
                     nuclease/putative transposase"
                     /protein_id="HRX16140.1"
                     /translation="MLTDHLILHFIELPKVGKNMVKSSLEMWSQYFINEGGENNIMKT
                     LLKDEPIIEKAHNEFKKFTADEHLREIYESRLKAQRDHAYFMEVAREEGLKEGIEQAV
                     IETAQKMLADGLAVDVISKYTGLTVEKINTLQSK"
     gene            5631..6722
                     /locus_tag="P5123_07495"
     CDS             5631..6722
                     /locus_tag="P5123_07495"
                     /inference="COORDINATES: protein motif:HMM:NF024242.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HRX16141.1"
                     /translation="MLTDVITILATCAAILQIITILLQSRTYRSVLLSLLIFSLAYLN
                     FYLFVVTKGLLSDYPFILFTNIIVAVYIGPLFFLYIKTALSENRGFKKKDLLCFIPSI
                     IVCISLLPMLLLPAHQKIVLNSSFATANGFFPLRLFVIISIANMLIFLFLAFYIYFFK
                     TRRIGFIFKKFRSNIVLVVSAIVLIAVFMINGLFSFFNMLGGFFLKTEPYNFIFSIII
                     LGVYFLNQRFLFVFLYGSLTGKKRSKNLLLKINKEKLESRLQTIMCDQRLYCNEDMNL
                     STLSKIAGVGTHQLSQYINDVHGDNFNGYINSFRINEAKRLMLEEPQAEIISISFSVG
                     FNSYSAFSSAFKKITGTSAANFRAMIKKK"
     gene            6894..7388
                     /locus_tag="P5123_07500"
     CDS             6894..7388
                     /locus_tag="P5123_07500"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRX16142.1"
                     /translation="MHKIKLMTLFICTILVYTAFHISEEAFGNFPLFMQQNWGISNIG
                     YARWLFHNAVFFVPVLVGGLLVYSINEKRMLPFGVGITLWGVINFCEHLFYTIKNSEV
                     SPGTFSSLIFIVLGVLVYLKLRKTEGIGMKTAGLSVLCALLYWGLPLFLIIALSNPVG
                     RLFG"
     gene            7599..>9607
                     /locus_tag="P5123_07505"
     CDS             7599..>9607
                     /locus_tag="P5123_07505"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fibronectin type III domain-containing protein"
                     /protein_id="HRX16143.1"
                     /translation="MKNIILCAFEYFKGFHVCFFVVMTSCFLVFSCYGPDMFDLANSD
                     ASKGSPEPPVEDVNNGNNMQVTLDSVISGYGFVKIEWIDPDTENFTSINIYDESDDSL
                     ETSVAPGVQQVTIDGLKNGTTYAFLLRVQDDAGNLGPEVRVTAIPDTTDKDYILIHNA
                     QELNDVRNDLAADYLVVEDINLAGAYTPWVPIGNNSSPFTGTFNGNGHTIRNLKIIAN
                     HMNKGLFGYIGDGSAVVKIMDVVLTGFELTASNTSSVEYIGSIAGYCYGAIIQDCDVS
                     GNISVEGKAYIGGITGYNSAGGIIENSESDVVIEGSYVNNNPVGGLVGGNRGLITNSV
                     ARVDIDVNTRIGTGGIAGSSVSGATITYCSVNVKKIRSNEKVGGIIGANNGVVNYCNV
                     NSDNGSITGTKWVGGFVGTYGEGYVTGPSSVNLDLVSGSEYVGGFAGQVSVGGINDSI
                     SIKVGKVKGAAYVGGFAGQIGSDTSLSPVFVSVDDVSVQADVEFGGSSSVSDTDNGIG
                     GFAGYITKTDNCLISGSSFIGSVTAPTKDNVGGFIGKTYKSTVTIEKSRVKSGPIEGK
                     DNVGGFFGFVTTEGADTDLKVKTCYSNSNISGKDYVGGFVGNIGKEDAFVLKITFNDT
                     ASYGTVVGGSASTVGGFAGSMAGCGGDSKITRAYSRAEVDGFTMV"
BASE COUNT         2808 a         1597 c         2148 g         3054 t
ORIGIN      
        1 gcgttatcag ggcgtttatc ccataaaggt taatcagcaa aagcaggttt tgaaagaagt
       61 tacccgcttt ggcgaacgct atcaccacgg gttagaggtg ggtagtaagc ctgaactaat
      121 agctgcgctt tcggttatga aagacaaaga ggcctgtctg gtatgtaatg gttacaagga
      181 tgaagagttt attgatcttg ggctttacgc gacaaagatg gggtttaaat gtttttttgt
      241 cgtcgaaata cccggagaag ttgatatgat actgcaacgt tcccgcaagc tgggaataac
      301 tccaaacatt ggtgtgcgta taaagcttgc ttcgagagcg ggtggtcact ggaccgaatc
      361 gggtggcgac cgcagcatat tcggtcttaa cgtttcacag ctaatcgatg ttgttgacct
      421 gttaaaaaaa gaggatatgc ttcacgcgtt aaagctggta cattatcatc ttggttcgca
      481 gattcctaat attcgtgata ttcgttcggc ggtggtcgag gctgctcgag tatatgctga
      541 gcttgtcaag gagggggcgc ccatgggtta ccttgatctg ggtggcgggc ttgctgtcga
      601 ttatgacggt tctaaaacaa attacagctc ttcgcgtaac tattcgttgt cggaatattg
      661 tgctgatata atagaagttg ttatgagtat aatgaacgag catgagatag agcacccaac
      721 tataataaca gaatcaggtc gtgccattgt cgcatattat tctgtgcttc tttttaatgt
      781 gctcgatact tcgacctttg aacatcctgt tgtaccagat acacttgatc cggaaactca
      841 tgaacagata attaacctgc atgatgttta taaaggactt acattaaaaa acctgcaaga
      901 gagtttcaat gacgcaatct attacaggga tataatacgt gatcatttta agcacggtca
      961 actctcttta cgcgaaaggt ctcttgctga atctatattc tggaatatta ttacgtatat
     1021 agcagccgag aagagcaggg taaagagggt tccgcaagag cttgacggaa tcggagacgc
     1081 tattgccgat atttattact gcaatttctc ggtatttcaa tctgtacccg attcgtgggc
     1141 aattgatcag ctcttcccta taatgccgat acaccgttta gaagagatgc ctactcgcaa
     1201 ggccgttatt gccgatatca cctgcgattg tgatggcaag atagaccggt ttatagatac
     1261 tcatgatgta gggcacactc ttagacttca tgctttaaat aacggcagcg actactattt
     1321 gggtgtgttt ttagtcggag cgtaccagga gacgcttgga gatttgcata atctttttgg
     1381 tgataccaat gttgtgagta tacagattaa taaatctggt ggttttgata ttgtgcgtga
     1441 gatcgaagga gactcggtag aggatgttct ttcgtatgtt gagtatgata caaaacgaat
     1501 ggtagaagac tttaaacaat cggcggaaga ggcaatacgt gaagggcgaa ttactgcggc
     1561 tgatcgcaga aatattatta atgcttacca gaacggatta cgtggataca catattatga
     1621 aagattcgag gagtagcaga tgggaagtgt agtaattatt ggtgccggcg gagtcggcca
     1681 ggttgtggct cacaagtgtg ctcaggtacc tgaagttttt gaaaagataa cgttggcaag
     1741 ccgtacaaag tctaagtgtg atgccatagc ggcgggtatt gaccgtaaaa ttgatacggc
     1801 tcaaattgac gcggatgatg ttccggcgct taccgaattt ttactaaaaa taaaaccaga
     1861 cctggtaata aatgtggctt tgccttatca ggacttgcat ataatggacg cctgtttggc
     1921 tgccggtgtt cactatcttg atacggcgaa ctacgagcca cttgatgaag caaagttttg
     1981 ctacaagtgg cagtgggagt atcaggagcg tttcgagaag gccggtttaa cagcgctttt
     2041 aggttcgggt tttgatccgg gtgtaacaaa cgtgtttacg gcatacgcag ccaaacattt
     2101 ttttgacgag ataaacgagc ttgaaataat tgatgttaac ggtggtgatc atggtcagcc
     2161 gtttgcaaca aattttaacc ccgagattaa tattcgtgag gtaacagcac cctgtaaaca
     2221 ctgggaagtt gataaatttg tagagacaga ggccatgtct gtaaagaaaa gttttgagtg
     2281 cccagaaaac gtgggaactt acaatattta cagaatgtac cacgaagaga tggagtcgct
     2341 tgttaagcat tttccaacga ttaagaaagc atcgttttgg atgagctttt cggataacta
     2401 tctgaagcat cttgaggtgt tgggcaatgt gggtatgacc cgaattgatc cggttatgta
     2461 caacggccag cctgtcgttc cgttacagtt tttaaaagct gtgcttcctg acccgtcttc
     2521 gcttggtcca cttacaaaag ggcgaacttg tatcggttgc gtatgtaccg gtaaaaagga
     2581 tggtaaagag cttaaaaaat atatctataa tatatgtagt catgaagatg catttaaaga
     2641 ggttaagtct caggcaatat cgtataccac tggtgttcct gcgatgatag gagccatgat
     2701 gatgctgacc aaaaagtggt taaagcccgg tgtttttaat atggagcagt ttgatcctga
     2761 tccttttatg gatgctttaa acaaacatgg attgccatgg aaggagacaa cgttatagac
     2821 gcacaaacag tacaaaaata taatcagttt cagactccct gctttattgt agagcgggga
     2881 cgtcttttaa aaaactgtga gacattagat accgtaaaaa aaagaaccgg tgcaaaaata
     2941 cttttggcgc tgaaaggttt tgcaatgtat tcggtttttg atgacatagg cgattacctg
     3001 gacggtgttt gcgccagctc accgcatgag gcgcggcttg gacgtgaaga gtttaaaaaa
     3061 gaggtgcacg catttgcggc agcttattca caaaaagatt acaacgatat atgccaattt
     3121 gccaatcata ttgtgtttaa ctctattgat cagtataaac ggttcgagct aaaagacaca
     3181 atcgactatg ggcttcggat taaccctcgc cactcggaga tagatgtcgc catttatgat
     3241 ccctgtgctc ctttttcacg cttgggtata atcagcgata atttaccgga cagacttcct
     3301 gaaggaatta ccgggctgca tatgcatacc ttatgtgaaa agaacagcga cgcatttagc
     3361 cgtactctga tggctgccga agaacagttt tcagaacagt ttaagcagtg cagttggatt
     3421 aatttcggtg gtggacatca tataacgcgc caagattacg atattgatct gttatgtgat
     3481 accttaaacg aatttaaaac acgttataaa gccgagcttt atcttgagcc gggcgaagcg
     3541 gtagccctaa atacgggttt tcttacggca acggttcttg atgttgttga taacggaatg
     3601 aaaattgcaa ttgtcgatac gtcggcagct gcacacatgc ccgatgtcct tgagatgcca
     3661 taccggccat acattattgg atcgggtaaa ccgcatgaga aagcgcatag ttaccgccta
     3721 ggcggtttgt cgtgtcttgc cggagatatt ataggcgatt attcgtttga taagccgctt
     3781 gctgttggtg acaaactgat atttacagat atggcacatt actcaatggt caaaaccaat
     3841 acctttaatg gacttacctt gccggacatt atctattacg atgaggcaaa tgataagatt
     3901 cttcatcaca aaacatttgg ttacgaaaac tttaaaaaca ggctctctta aagaggtcca
     3961 cgaaatagga tttcattatg aaaaaaattg ctctgatagt tgctgtaaaa accgaggcgc
     4021 gcgaactttt agccgactcg ttttggggat gggaaaaaaa agagccgatg cattactgct
     4081 catcgcgttt tcctgtgaca cttactatca gcggaatagg caaggtcttt gctacttacg
     4141 ccatgtcaca ggcggcaaac ggagcagata cggttttgag cgtgggtact tcgggaggac
     4201 ttggcaaaca gagagttggc gatctttaca tctgccatga gtttgtagaa catgatattg
     4261 atcttacaca tcttggaata ccttttggtg tgactcccta tgacgcaatg gattcgcccg
     4321 ttatttctcc agagactgat aagttgcttg aagagcgtgt tatagcagcc attgacgagc
     4381 ttggattgac tcacaatttt ggaaggtcga tgagcggcga tcagtttatt acggataaag
     4441 agataatcgg tcacaagata gagactttca gtgccgatct gacagatatg gaaagcagtg
     4501 cctgcgctaa attggcggct taccgtatca aaatcccatt cttcgcgctg cgttatgtaa
     4561 gtgataatgc agatcacaac gcctctgtca gctggaccga agaggtaaaa aaggccgctg
     4621 ttattttaaa cgatgtttta aaacttgcag taaactcgat ggattgttag ccataaattg
     4681 atcttaatta caagaagagg tcattttgat tgtaaagtat taatcttctc aacagtaagc
     4741 ccagtgtatt tggagattac atcaacggca agtccgtcag cgagcatttt ttgagctgtt
     4801 tcgataactg cctgttcgat accctctttt agaccctctt ctcttgcgac ttccataaag
     4861 taggcgtggt ctctctgagc ctttaatctt gattcgtata tctcacgcag atgttcatca
     4921 gctgtaaatt ttttaaactc attgtgagcc ttttcgatta tcggttcgtc ttttaataaa
     4981 gttttcatga tattgttttc tccgccttca ttaataaagt actgactcca catttcaagg
     5041 gagcttttta ccatgttttt acctaccttg ggtaactcta taaaatgcag tataagatga
     5101 tctgttaata ttttgtctct tcacgtccta gcaggaactt aactacaata tctgaatatg
     5161 cttttatgct atccatggtg tgtttctcaa aacctcattg ttaaaataaa aaatatgctg
     5221 aatttcaaaa agaatgtgca acaaaagtac gagaaagttt atggctaatc atgtaatttt
     5281 aaaattactt tgttttatgc gaaagtcaag acttatttgc aggttgattt tgtctatgtt
     5341 gtccatgttg ttaccgttca ataatagtca gtttttcaaa gatgatttga acacttctcg
     5401 tcaatttcta attatttttg ctattgacat acgatgactt ttaacaatta cataaattgc
     5461 tctaaaaagg attagcatta atattattac agaaaaaact gtgatttgaa aagagaaaag
     5521 ctatttatat taaagattta gagtaggctg gatattaaac aaaagctcgg atttcccatt
     5581 gttcagatga agacaacatg actaaagaaa atttaagaat tcggtaaaat atgttaactg
     5641 acgttataac aattttggct acatgtgccg ctattttgca gataattaca attttgcttc
     5701 aatcgcgaac ataccgaagt gtgcttttgt cacttttaat attttcactc gcttatctga
     5761 atttttatct gtttgtcgta acaaaaggct tgttaagtga ttatccgttt attcttttta
     5821 ctaatattat tgtggctgta tacattggcc ccttattttt tttatatata aaaactgcgt
     5881 tatcagaaaa caggggtttt aaaaaaaaag atttattatg ttttattcct tcgataattg
     5941 tttgtatatc tttgcttccc atgttgctct tgccggcaca tcaaaaaata gttcttaata
     6001 gtagttttgc taccgcaaat ggtttctttc cgcttcgact atttgtaatt atttctatag
     6061 caaatatgct aatttttctg tttctggctt tttatattta tttctttaaa acaagacgaa
     6121 taggattcat atttaaaaaa ttcagatcaa atatagtttt agttgtatcc gcgattgtgc
     6181 taatagccgt tttcatgatt aacggtctgt tttcgttttt caatatgttg gggggatttt
     6241 tcctgaaaac agaaccatac aattttatat ttagcattat aattctcggc gtttactttc
     6301 ttaaccaacg atttcttttt gtctttttgt acggatcatt gacaggcaaa aaacgatcaa
     6361 aaaatctttt gttaaagatc aataaagaaa aactggaatc gcgtttacaa acaatcatgt
     6421 gtgatcagcg tctgtattgt aacgaagata tgaatctttc tacactttcc aaaattgcag
     6481 gagtaggtac ccaccagctt tcacagtaca tcaatgatgt gcatggcgat aattttaatg
     6541 gatatattaa cagttttaga ataaatgaag caaaacgcct gatgttagaa gaaccacaag
     6601 cggaaataat ttcaatttcc ttttccgttg gttttaattc gtactcggct ttttcctcgg
     6661 cgtttaaaaa aattaccggc acttctgctg caaactttcg cgcaatgatt aagaaaaagt
     6721 aaaagagatt aagaaaaatt attttgagtg aaaccttgga aatcttctgt tttgataaat
     6781 tatttttgtt aagtaacgga attataaatc aggtagaaac cgtactaaat attgtgatat
     6841 gataatgtgc aattaccgtt aaatttggcg gaatataaaa atggagttaa gtgatgcata
     6901 aaattaaatt aatgactctt tttatctgta ccattttggt ttacactgcc tttcatattt
     6961 cagaagaggc ctttggcaat tttccactgt ttatgcagca aaattggggt atctctaata
     7021 taggttacgc gcgttggctt tttcataatg cagttttttt tgtaccagta ctagtgggtg
     7081 ggcttcttgt ttacagtata aatgaaaaga gaatgcttcc ttttggggtc ggaattactc
     7141 tttggggtgt tataaatttt tgtgaacatc tgttttatac aattaagaat tctgaagtat
     7201 caccgggtac tttttcttca ctgattttta tcgtattggg agtattggtc tatttaaaac
     7261 ttagaaagac cgaaggcatt ggcatgaaaa cagccgggtt atcggtttta tgtgctttat
     7321 tatattgggg gcttcctctc tttctaatta ttgctttaag caatccagtt ggaagactat
     7381 ttggttaaga tgtcctgttt atctttattt tgaatacggg gtattattta ataataaaac
     7441 cggttgcaaa gataaaataa aaattttttt cttgaatata tcatataatt agataaaaaa
     7501 tgatacatat tatattaaaa gctgtgtgtg gccaatcaga aacggattca gatgacatta
     7561 ttgaacaaga aacgcaggac aatacgttgg agtgtattat gaaaaacatt attttatgtg
     7621 catttgaata ttttaaggga tttcatgtct gtttttttgt tgtaatgacc tcatgttttc
     7681 ttgttttttc gtgttatgga cctgatatgt ttgatttggc aaacagtgac gcttcaaagg
     7741 gttcacctga acctcccgtt gaagatgtaa ataatggaaa taatatgcag gtgacacttg
     7801 atagtgtaat aagcggatat ggctttgtca aaatagagtg gatcgaccct gatactgaaa
     7861 attttactag tattaatata tatgatgagt ctgacgattc gttggaaaca agcgtggcac
     7921 ctggtgtaca acaggtaacc attgacgggc ttaaaaatgg tactacatac gcctttttat
     7981 taagagtaca ggatgatgcc ggtaatctgg gaccagaggt aagggtaaca gctataccgg
     8041 ataccacaga caaggactat atacttatac ataatgctca ggaacttaac gatgtacgta
     8101 atgatcttgc tgccgattat cttgtagtcg aggatataaa tcttgccgga gcatacacac
     8161 catgggttcc tattggtaat aattcttcac cttttaccgg aacctttaat ggaaacggtc
     8221 atacaatacg aaatctgaag attatcgcca atcatatgaa taagggctta ttcgggtata
     8281 ttggtgatgg gtcagccgta gtaaaaatta tggatgttgt gctgacagga tttgaactta
     8341 cggcatcaaa cacaagttct gttgaatata taggatcaat tgctggttat tgctatggtg
     8401 ctataataca agattgtgat gttagtggta atatttctgt tgagggaaaa gcatatatag
     8461 ggggtattac agggtataac tctgctggtg gtattataga aaatagtgaa agtgatgttg
     8521 ttattgaagg gagttatgtc aataataatc cggtcggtgg attggttggt gggaatcgtg
     8581 gtttaataac gaattcggtt gccagagtag atattgatgt taatacgaga attggtaccg
     8641 gcggaattgc cggatcttct gtatcaggtg ctacaattac ctattgtagt gtaaatgtaa
     8701 aaaaaatcag atcaaatgaa aaggtcggtg gtataattgg agcaaataac ggggttgtta
     8761 actattgtaa tgtaaacagc gataatggca gcattacagg gactaaatgg gtcggtggct
     8821 ttgttggtac ttatggcgaa ggttatgtta caggtccttc atcagttaat ttagatctgg
     8881 taagcggatc tgaatatgtg ggtggttttg ctggtcaagt atccgttggt ggcattaacg
     8941 attcaattag tattaaagtc ggtaaagtta aaggtgcggc ctatgttggc ggttttgccg
     9001 gtcaaattgg ttcggataca agtctgagtc ctgtttttgt cagcgtcgat gatgttagtg
     9061 tacaggcgga tgttgagttt ggaggttcat cctctgtttc ggatactgat aacggtatag
     9121 gtgggtttgc cgggtatatt actaaaacag ataattgctt aattagtggc tcatccttta
     9181 tcggttcggt taccgcgcct acgaaagata atgttggtgg ttttatcggg aaaacataca
     9241 agtcaacagt tacaatagag aaatctcggg taaaaagtgg cccaattgaa ggaaaagata
     9301 atgttggtgg attttttggt tttgtaacta ctgaaggagc tgacaccgat ttaaaagtca
     9361 agacctgtta cagtaattct aatattagtg gtaaagatta tgttggtggc tttgttggta
     9421 atataggcaa ggaagatgct tttgttctta agattacgtt taacgatacg gcgtcttacg
     9481 gtacggttgt tggtggatca gcgagtactg tgggaggatt cgctggcagc atggcaggat
     9541 gtggaggcga ttcgaaaatt acaagggcgt attcgcgtgc cgaagtagat ggattcacaa
     9601 tggtaat
//
LOCUS       DAOTGZ010000094         9485 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_3064
            NODE_8599_length_9485_cov_4.286320, whole genome shotgun sequence.
ACCESSION   DAOTGZ010000094 DAOTGZ010000000
VERSION     DAOTGZ010000094.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868088
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 9485)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9485)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:22:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,785
            CDSs (total)                      :: 2,751
            Genes (coding)                    :: 2,732
            CDSs (with protein)               :: 2,732
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 12 of 19
            Pseudo Genes (internal stop)      :: 5 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9485
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8599_length_9485_cov_4.286320"
                     /isolate="AMR_MDS_3064"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..2454
                     /locus_tag="P5123_07510"
     CDS             <1..2454
                     /locus_tag="P5123_07510"
                     /inference="COORDINATES: protein motif:HMM:NF019335.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015267 - channel activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HRX16144.1"
                     /translation="MRRLFLNTILLITVLSGSASVFAQGAPRLTGSVKSAVTGAPVDF
                     GMVIVFEAQRRGMIGPDGTYDIALPNNGSFTVEIRVEGLKPSRQTVDVRGVTVHNATL
                     QPMSIAGAGVTIRGDRKQDLSRRSMTVEELNAVPATLGDSVSALTSLPGVTRYSGGGG
                     GGSNMYGQMTVRGMEPNSNRFFVDGVPIQYPQHFFGLHAVVANPLISTIDLYSSAHPA
                     EFNGAMGSVIQINTLDKVEEFWGVVEMNLLSATSAVWTPTYSNTYQNGQLKKERNGYF
                     IVAGRVGYLSLFVPIIYELIQGEELDQVPNYWDYQIKYKHYFNSRNSLTLMAMGAKDW
                     WDVRLSSTFMDDTMDPLIHGAELDTDQMFNNQMAVHKYTTGKVTNRLTGYSALPTYMN
                     NFGLNHDLVADAFRDIKMKTMPYEFGIKDELFFDYMEGMGQLAMSAGYTHYRFSQKGD
                     VVLMRELPESGEIDYLKEDSLFYRTEVDKTYTNNLFHGHLSNSLTFGGLTVIPQVRLE
                     YLDRAESFVADPRGLVKYEFPSRTTVSVASGLYHEFVQVNPYMFNMNTEFANMGKEVK
                     PERSVQNVLGVKQEMGLYTLSVEGFYNYYYDMLRDWPHEVDGKLVPVKNTGEKKTIGA
                     EIMLKKDALANVNDYFGWISYTFTQSEERDNVPISVDKTGKGENWYTSPYEQKHAIKL
                     MTGYKMGNHLFSAQFQFYTSHPYTRIIGSVEDEEYKEYMAEKGEDKERHVPVYSDEPY
                     TEHNTPEFQLDIRYTYNAKYKWGNVKWYIEVVDVLSMFNDTYTYTYYYNKPYSKSNPT
                     NLKDEGIGTIPNMGVEISF"
     gene            2498..>2717
                     /locus_tag="P5123_07515"
     CDS             2498..>2717
                     /locus_tag="P5123_07515"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRX16145.1"
                     /translation="MRFRAASPKNYHNYKTFTVFRVEVLCGFGRRARKTTITIKLSLS
                     LEWKFYAVSGDSPKNYHNYKTFTVFRVEV"
     assembly_gap    2718..2817
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            3198..3665
                     /gene="tadA"
                     /locus_tag="P5123_07520"
     CDS             3198..3665
                     /gene="tadA"
                     /locus_tag="P5123_07520"
                     /EC_number="3.5.4.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010246481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008251 - tRNA-specific adenosine
                     deaminase activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0002100 - tRNA wobble adenosine to inosine
                     editing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA adenosine(34) deaminase TadA"
                     /protein_id="HRX16146.1"
                     /translation="MDKCDKDKFMREALVLASQAGEMGEIPIGAVIVKDDIIIGRGAN
                     CNRTRNNPTRHAEIIAIEEACEKLSNERLIGCSLYVTKEPCTMCAGAIIHARIAEIYF
                     GASDEKYGACGTVFNICGNDQYNHVPVIYKGMLKNDSSLLLKDFFKRLRDEKN"
     gene            3652..4356
                     /locus_tag="P5123_07525"
     CDS             3652..4356
                     /locus_tag="P5123_07525"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRX16147.1"
                     /translation="MKKINVLRLFLSAAILTNRNILSLLRVNLLLLSVIFFVSYCAAV
                     AGIKITYDRRLQELLAITISFPFISYLFAGAVYYYLSYYKGVTVRLNQIYMGFRWFAN
                     FSKLGFVLLIVYYMVIIPVFSFNEYNLEEKIRVGTGTLFFLWFLVRNFFLPILFVDFD
                     DSFEELLFKSLSLSRGYVFRIIIFIAASFVVVSAGLAIAGFGVLYSISVVALAYIRFY
                     YLVRSREDEKIKIHSL"
     gene            4328..5065
                     /locus_tag="P5123_07530"
     CDS             4328..5065
                     /locus_tag="P5123_07530"
                     /inference="COORDINATES: protein motif:HMM:NF018894.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0035438 - cyclic-di-GMP binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PilZ domain-containing protein"
                     /protein_id="HRX16148.1"
                     /translation="MKKLKYIVFERRRRNKRPFLLKIFTMLILFIPAYYYAQKIIAFD
                     FFYQDYRNIIAAFSIEELVISLCAIIIIILLLIGIRIGFYLVLVSLLLFVFYQLYLFF
                     ITGQIFNFEPVLMSFGGAAFFIYMTSPDNIAPFVRRRTKGFRKELRRTFNHTVVVDNK
                     KKVIENISSRGMLLRWKDSGLKPCNELELSLVLNRHKFEIKAGIVWSDSDVVALAFRS
                     LDRSVRDAVYLRLKHLEEKRSNFNETV"
     gene            5052..6461
                     /locus_tag="P5123_07535"
     CDS             5052..6461
                     /locus_tag="P5123_07535"
                     /inference="COORDINATES: protein motif:HMM:NF012707.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter substrate-binding domain-containing
                     protein"
                     /protein_id="HRX16149.1"
                     /translation="MKLFKISVILLFMLGYSCIAILDRMSGVTVEKIKERGKLIAVTS
                     YGANNYFLYRGEPFGFEYELLRLLADDLGVKLEIQLTDGRVPLMDILNSDYGDIVASS
                     ITITKSRRKQYNFTRHFMKTKQVLVQRLGRDGDIVQDVTDLIGKEVVITENSHYYQRL
                     QNLSEEIGGDILIKTVPPQIGEEELIRMVNDGSIDFTVADETIAMINNFYYPDVDINT
                     PISFPQRIAWVVRRKSPSFRNYVNDWLSRIEADGTLEELYNKYYRINRVLEEQLSFSQ
                     DPGNKAIISEYDGLLRKYSYIINWDWRLIAALIHQESRFNPRARSWAGAAGLMQLMPK
                     TAEILGVTDRYNPEQNVFGGIRHLKGLIDIWADRVPGETNRLKFVLASYNAGVGHISD
                     AQRLAEKYGKDPLIWENNVDEYVLKLSNPEFYNDAVVENGYCRGSEPYNYVRVILNRY
                     QIYKKNIPLIPESEGAASK"
     gene            6458..>9485
                     /locus_tag="P5123_07540"
     CDS             6458..>9485
                     /locus_tag="P5123_07540"
                     /inference="COORDINATES: protein motif:HMM:NF012298.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HRX16150.1"
                     /translation="MIQIVGYSDLQLISESGKKLIYRAFDIKNNRHVILKILRQGGRT
                     VDDVRHVRDEYYLLKDIDIHSVIKAYSLIESNSVLGIVYEDFDGITLAEYFSESPFKE
                     GFFLIACSIARALADLQLYKVVHLNINPTNILIDPKTKIIKIIDFSAARSYQKVLQLQ
                     GGEFAASSQAAYIAPEQTGRMNCAVDYRADFYSFGVTLYELACGVRPFDFEDPIVLFH
                     SHIARRPENLSSVNPLIPVVFSRIVSKLLEKMPDQRYQSAAGIIADLEKCRKEFEENN
                     YIHDFEIAQNDVSPEFMIGNMPFGREREIKILKNSFDWIRNGHTEMVFVTGASGVGKS
                     YLIKELDSYSAQSNGLYLSSHCDPFNQNTPYYALRLLLNDFAGQILLKSDKEITDWKR
                     VICDKLGDNIGLFTEFVPDFSAIIGKTDTPDLSEIEREVRSRILFQQVFAVISSKEHP
                     LVIVIEDIEWLDSASLNFLHLIMLSREHTYMLIVTSARKTLSEYHPFNSMVRDINQNG
                     NRIVQTISLDELSVNELNELLAATLRCSCNDSIELSKQIFNKTGGNPFFIREFITTLY
                     KEGVFQFSVEDGIWSWNSGAIESAAFSGNMLDIMVDKIETLDKAQKEPLIFAACIGVT
                     FSLEILSELCRKSREYIESAIQSAIEAGLVNYYGTEYRFTHERIREAARSVLSDEQMQ
                     VIHRQIGLFLLDKKQGDFEQNYFSVVNHLNYSIPLITEEEERYFLAALNSEAGRRAKL
                     SAAYKNSYRYYQTGVKLLPEQSWQNCYELTYELNRGAADAALLAGEHKLADSYANAII
                     DNSENFIHKVGVYSIIINARISQNRLSEAVNIAFMVLSLLKIEIPKSDRTVEKRLKSI
                     HRGIKIFSHIWKRFSAFGDMPLMTNETMLSASSIIARVSGTALISRPDLYPLLASFHI
                     ELLRKYGNSPYAPQAYAEYGAYLSSIQKYDAGLDMVNMAMKMCEDSRYTHVLPRVLFY
                     HSALLDHPDIDRDEMIFTLAKAEKLARERGDFEYSAL"
BASE COUNT         2781 a         1548 c         2063 g         2993 t
ORIGIN      
        1 atgagaagat tatttttaaa tactatattg ctgattactg ttttaagtgg atctgccagc
       61 gtatttgcac aaggggctcc aaggttaaca ggttcagtaa aaagtgctgt aacaggtgct
      121 ccggtggatt ttggaatggt aatcgttttc gaagcacaaa ggcgcggtat gattggacct
      181 gacggcactt atgatattgc gttaccaaat aatggcagtt ttacggtaga gatcagggtc
      241 gaaggtttga agccttctcg tcagacagta gacgtcagag gggttactgt gcataatgcc
      301 acattgcagc cgatgtctat tgctggcgct ggagtaacca taaggggcga tcgcaagcag
      361 gatctttcaa gaagatcgat gacggttgaa gagctgaatg cagttccggc tactctggga
      421 gattcagtga gtgctcttac ttcgcttccg ggtgtaacaa gatattcggg cggcggtggc
      481 ggtggttcta atatgtacgg gcaaatgact gtaaggggta tggagcctaa ctcaaacagg
      541 ttttttgtgg atggtgttcc tattcagtat ccgcagcact ttttcggact gcatgcggtt
      601 gtggcaaatc ctctgatttc gactattgat ctctattcgt cggctcaccc ggctgaattt
      661 aacggcgcta tgggctcggt tatacagata aacactctcg ataaggtcga agaattctgg
      721 ggagtagttg agatgaatct tcttagcgcc acttctgctg tttggactcc gacatattcc
      781 aatacttatc agaacggtca gcttaaaaaa gagagaaacg gatattttat agtagcgggt
      841 cgtgttggtt atcttagcct gtttgttccg attatatatg aactgataca aggtgaagag
      901 ttggatcagg ttcctaatta ctgggattat cagataaagt ataagcacta ttttaacagc
      961 cgtaattcac taactcttat ggcaatgggg gccaaggatt ggtgggatgt tcgtttgtcg
     1021 agtactttta tggatgatac aatggatccc cttatacatg gtgctgagct tgatacagac
     1081 cagatgttta ataaccagat ggcggttcat aagtatacaa ccggaaaggt aacaaatcgt
     1141 ctaacaggat actccgctct acctacctat atgaataatt ttggtttaaa tcatgatctt
     1201 gtcgcggatg cttttcgtga tattaagatg aagacaatgc cgtatgagtt tggaataaag
     1261 gatgagctgt tctttgatta tatggaagga atggggcagc ttgccatgtc tgccggatac
     1321 acacattaca gattttcaca aaagggtgat gtagtattga tgcgggaact acctgaatcg
     1381 ggtgagatcg attatcttaa ggaagattcc ttgttttaca gaacagaagt cgataaaaca
     1441 tatactaaca atctttttca cggacatctt tcaaatagcc tgacatttgg tggtttaact
     1501 gttattccgc aggttcgtct ggagtacctg gatcgggcgg agtcttttgt tgctgatcct
     1561 agaggtcttg ttaagtatga gtttccatca cgcactactg tatctgtagc gtccggatta
     1621 tatcacgagt ttgttcaggt gaatccctat atgtttaata tgaacacaga attcgccaat
     1681 atgggcaaag aggtgaagcc ggaaagatct gtgcaaaacg tattgggtgt aaaacaggaa
     1741 atgggtcttt atactttatc tgtagaggga ttttacaatt actactatga tatgcttcgt
     1801 gactggcctc atgaagttga tggaaagttg gtacctgtca aaaatacggg agaaaagaag
     1861 acaatcggtg cggaaatcat gctcaagaag gatgctcttg caaatgttaa tgattatttt
     1921 ggatggatca gttatacctt tactcaatcg gaagaacgcg ataatgtacc aataagtgtc
     1981 gacaagaccg gaaaaggaga gaactggtat acttcgcctt atgagcagaa gcatgcgatt
     2041 aaactgatga caggatataa gatgggtaat cacctgtttt cggcacagtt tcaattttat
     2101 acttcacatc catacacgcg cattatcggt agtgtagaag acgaggaata taaggagtat
     2161 atggccgaga aaggggagga taaagagaga catgtccctg tttattctga tgaaccatat
     2221 accgaacaca atactcctga atttcagctt gatattcgct acacctataa tgcaaagtat
     2281 aaatggggca acgttaaatg gtatattgag gttgtcgatg tgttgagcat gtttaatgat
     2341 acatatactt atacatatta ttacaataaa ccatattcaa aaagtaaccc aacaaatctg
     2401 aaagacgaag gaataggaac catcccaaac atgggagtag aaatttcgtt ttaattagtt
     2461 agcataaaac tttcactgtc tttagagtgg aagttttatg cggtttcggg cggcgagccc
     2521 gaaaaactac cataactata aaactttcac tgtctttaga gtggaagttt tatgcggttt
     2581 cgggcgtcga gcccgaaaaa ctaccataac tataaaactt tcactgtctt tagagtggaa
     2641 gttttatgcg gtttcgggcg atagcccgaa aaactaccat aactataaaa ctttcactgt
     2701 ctttagagtg gaagtttnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2761 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnngtc
     2821 tttagagtgg aagttttatg cggtttcggg cgatagcccg aaaaactacc atgttgcaat
     2881 aaaaccttta ccgtcctcaa ttctcgcata tctaactgaa ttacaaagtt gacaaaacag
     2941 ttcttaatca ttattttatc ttaaattcgt tctgaacaga gctgattata tcgttatttg
     3001 aattactaaa atacttagaa tagtaactat gtaaacattt agaatatttt tattaattta
     3061 aacaataaaa tctgtttgtt gtctgatata ggctactgat ttattgaaag tttcaatgat
     3121 tttgtaatcc attgctcttt ttaagtaaaa atattgttct tttatgtttt ctatgattaa
     3181 taataatgag tttaaatatg gataaatgtg ataaagataa atttatgcgt gaggcacttg
     3241 tccttgcttc gcaggcgggt gaaatgggcg aaatacctat tggtgccgta atagtaaaag
     3301 atgatatcat tatcgggcgt ggagcaaatt gcaaccgtac ccgaaataat cccacaagac
     3361 atgccgagat tattgcgata gaagaggcat gtgaaaaatt gagcaatgag agacttattg
     3421 gttgctccct ctatgtaaca aaagagccgt gtacaatgtg tgccggcgcg ataattcatg
     3481 ctaggatcgc agagatatat tttggtgctt cagacgaaaa atatggtgct tgtggaacag
     3541 tatttaatat ttgcggtaat gatcaatata accatgttcc tgtgatttac aaaggtatgc
     3601 ttaaaaatga ttcgtcgttg ctccttaaag atttttttaa aaggttacgt gatgaaaaaa
     3661 attaatgttt tacgcctttt tttgtcagca gcgattctta ctaacagaaa catactttct
     3721 cttttgaggg taaatcttct tctgctttct gtcatttttt ttgtgtccta ttgtgctgct
     3781 gtggctggta ttaaaatcac ttatgatcgt aggttacagg aactgttggc tataaccata
     3841 tcgtttccat ttatatctta cctttttgct ggagcggtgt attactatct ttcttattac
     3901 aagggagtaa ccgttagact taaccaaata tatatggggt ttagatggtt cgcaaatttt
     3961 tccaaactgg ggtttgtttt actgattgtc tattatatgg taattattcc ggttttttct
     4021 tttaacgagt ataatctgga agagaagatt cgtgttggaa ctggtacttt gtttttttta
     4081 tggtttcttg tcagaaattt cttcttaccg attctttttg tcgattttga tgactctttt
     4141 gaggagctgc tttttaaaag tttatcttta agccgtggat atgtttttcg aataatcatt
     4201 tttattgcag caagttttgt agttgtttcg gcaggtcttg caatagccgg attcggagta
     4261 ctctattcca tttctgtggt ggcattggcc tatataaggt tttattatct tgtcaggagc
     4321 agagaagatg aaaaaattaa aatacatagt ctttgagcgg cgacgaagga ataaaagacc
     4381 ttttctgcta aaaatattta ccatgttgat attatttatt ccggcatact attatgctca
     4441 gaaaataata gcattcgatt ttttttatca agattatcgt aatattattg ccgctttctc
     4501 tattgaggag ttggttataa gcctttgtgc cataatcata ataattttat tattaatagg
     4561 aataaggatc ggattttacc ttgtacttgt ttcattgttg ctgtttgtat tttatcagct
     4621 atatcttttt tttattaccg gacagatttt taattttgag ccggttttaa tgtcttttgg
     4681 aggagccgct tttttcatat atatgaccag ccctgataat atcgctccgt ttgttaggcg
     4741 cagaaccaaa ggttttagaa aagaactaag gagaactttt aatcatactg ttgttgttga
     4801 taataaaaag aaagtaattg aaaacataag ctctagggga atgttattga gatggaaaga
     4861 ctccgggctt aagccttgca atgaacttga acttagtctt gttttgaatc ggcataaatt
     4921 tgaaatcaag gctggcattg tgtggagtga cagtgatgtt gtagcccttg cgttcaggtc
     4981 tttggataga agtgttaggg acgcagttta tttacggctg aagcatcttg aagagaagag
     5041 gtcaaatttt aatgaaactg tttaaaattt cagtaatact tctttttatg cttggatatt
     5101 cttgtatcgc gatactcgac agaatgtcgg gtgtaactgt cgagaaaata aaagaaaggg
     5161 gtaaacttat tgctgttact tcttatggag caaacaacta ttttttgtac aggggagagc
     5221 cttttggatt tgagtatgag cttttacggc ttttagccga tgatcttggg gtaaaactag
     5281 aaatacaatt gacagatggt cgagtacccc ttatggatat attaaacagt gattatggag
     5341 atattgtagc ttcaagtata actataacaa aaagtcgtcg caagcagtat aattttacac
     5401 gccattttat gaaaaccaag caggtattgg ttcaaaggtt aggtagggac ggtgatattg
     5461 tccaggatgt aaccgatcta ataggtaaag aagtggtaat aaccgaaaat tctcactact
     5521 atcagagatt acaaaatctg tcagaggaaa taggtggaga tatactaatt aaaacagttc
     5581 cgccgcagat aggagaagag gaattaatca gaatggttaa cgacggcagt atcgatttta
     5641 ctgtcgcaga tgagacaatt gcaatgataa acaatttcta ttatccggat gttgatataa
     5701 atacacccat tagtttccca cagagaattg cgtgggtagt aagacgtaaa tcacccagtt
     5761 ttagaaatta cgttaatgat tggctgtctc gaatagaggc tgatggtaca ttggaggagc
     5821 tgtacaataa atattaccga attaaccgtg ttttggagga acaactctcc tttagtcagg
     5881 atccaggtaa caaagccatt atatcggagt atgacggcct gcttcgtaaa tactcatata
     5941 taattaattg ggattggcgt cttatcgcgg cgttgataca tcaggaatct cgttttaatc
     6001 cgcgcgcacg ttcatgggcc ggtgctgctg gtttaatgca gttgatgcca aaaacagcag
     6061 aaattcttgg tgtaacagac cgatacaatc ctgaacagaa tgtttttggt ggtatcaggc
     6121 atcttaaagg cttaatcgat atctgggccg atagagtacc cggagaaact aaccgactta
     6181 aatttgtttt ggcatcttat aatgcgggcg ttggtcatat tagtgacgcg caacgacttg
     6241 ctgaaaaata tggcaaggat ccgcttattt gggagaacaa tgtagatgaa tatgttctaa
     6301 aactgtcaaa tcccgagttc tataatgacg cagttgttga aaatggatat tgccgtggaa
     6361 gtgaacctta caattatgtt cgtgtgattt tgaatcgtta tcagatttat aaaaaaaaca
     6421 tacctttgat tccggagagt gaaggagcgg caagtaaatg atccagattg ttgggtattc
     6481 tgatttacag cttattagcg aaagtggaaa aaagctgata tacagagcct ttgacattaa
     6541 aaataatcgt catgttatat taaaaattct tcgtcagggc ggccgaactg tagacgatgt
     6601 acgtcatgta agagatgagt attatctttt gaaagacata gatattcact cagtaataaa
     6661 ggcctattca ctaattgaaa gtaatagcgt attgggcatt gtgtatgagg atttcgatgg
     6721 tataacttta gctgaatatt tttctgaatc cccctttaaa gaaggttttt ttttaattgc
     6781 ttgttcaata gcaagagctt tagctgattt acagctttat aaagttgtgc atttaaatat
     6841 taatccaaca aatattttaa ttgatcctaa aactaaaata ataaaaataa ttgatttttc
     6901 tgccgctaga tcttatcaaa aagtgttgca gttgcagggt ggagagtttg ccgcttcaag
     6961 tcaggccgca tatatagctc cagagcagac aggccgaatg aattgtgctg ttgattacag
     7021 agccgatttt tactcatttg gagttacgct ttatgaacta gcttgtggtg tccgaccttt
     7081 tgattttgaa gatccaatag ttcttttcca ttcccatatt gcgaggcgac ccgaaaatct
     7141 ctcttctgta aaccctttga tacctgttgt attctcaaga atagtatcaa aacttcttga
     7201 gaaaatgcct gatcagagat atcagagtgc ggctggaatt attgcggatc ttgaaaaatg
     7261 ccgtaaagag tttgaagaaa acaactatat tcacgatttt gaaattgcgc aaaatgatgt
     7321 gtcaccagaa tttatgatag gtaatatgcc tttcggacgg gagcgtgaaa tcaagatttt
     7381 gaagaactct tttgactgga tacgtaacgg tcatacagag atggttttcg taacaggtgc
     7441 ttccggagta ggtaagagct atcttataaa agaacttgat tcgtatagcg cacagagtaa
     7501 cggactgtat ttaagttctc attgtgatcc ttttaatcag aatacaccat attatgcttt
     7561 aaggcttctt ctaaatgatt ttgcgggtca aattcttctt aaaagtgata aagagataac
     7621 tgattggaaa agagttattt gtgacaagtt gggtgataac atcgggctct ttacagagtt
     7681 tgttcctgat ttttccgcta ttatcggtaa aaccgataca cctgatttaa gtgaaataga
     7741 acgagaagtc agatcgcgca tactttttca gcaagttttt gcagttatct catcaaaaga
     7801 acatcctctt gtaatagtga ttgaagatat agagtggctt gattcagcga gtctaaactt
     7861 tttgcatttg ataatgcttt ctcgagaaca cacatatatg ttgattgtca ccagtgcccg
     7921 gaaaacgttg agtgagtatc atccgtttaa cagcatggtg cgggatatta atcagaacgg
     7981 aaacagaatt gttcaaacaa taagtctgga tgaactctct gttaatgagc ttaacgagct
     8041 tcttgccgct actcttcgtt gttcttgtaa tgatagtatc gaactctcaa agcagatatt
     8101 taacaagaca ggtggaaatc cattttttat cagggaattt attacaaccc tgtataagga
     8161 aggagttttt cagttttctg tcgaagacgg tatttggagc tggaacagtg gtgctattga
     8221 aagtgcggcg ttttccggta acatgcttga tataatggtt gataagatag agacattaga
     8281 taaggcacag aaggaaccac tgatctttgc ggcatgcatt ggggttacat tttctcttga
     8341 gatattatcc gaattgtgca gaaaatcgcg tgagtacata gagtcagcga ttcagagtgc
     8401 tattgaagcc ggccttgtga attattatgg cacggagtat cggtttacac acgagcgtat
     8461 ccgtgaagct gcacgttcgg ttcttagtga tgagcagatg caggttattc acaggcaaat
     8521 cgggcttttc ctattggaca aaaaacaggg cgatttcgag caaaactatt tcagtgttgt
     8581 taaccatctt aactactcga ttccccttat tacagaagag gaagagcgtt attttcttgc
     8641 cgccttaaat agtgaagccg gacgaagggc aaagcttagc gctgcttaca aaaattctta
     8701 ccgctattat cagacaggtg tcaaactgct tcctgaacag agctggcaaa actgttatga
     8761 attaacctat gagcttaatc gcggggcggc ggatgcagca ctgcttgccg gcgagcataa
     8821 acttgcagat agttacgcga atgctataat cgataattca gaaaatttta ttcataaggt
     8881 tggagtctat tctataataa ttaatgccag aatatcgcaa aaccgtcttt cggaggcggt
     8941 aaatatcgct tttatggttc tttctctact taaaatagag atacctaaat cggaccgtac
     9001 tgtcgaaaag agactaaagt ccatccacag gggtataaag atattttctc atatatggaa
     9061 aaggttttct gcgtttggcg acatgcctct aatgactaat gagaccatgc ttagtgcgag
     9121 ctctataatt gcgcgagttt ccggaacagc tcttatttcg cgtcctgacc tttatccact
     9181 tttggcatcg tttcatattg aactgctgcg taaatatggc aacagccctt atgctccaca
     9241 ggcctatgcc gaatatggag cctatctgag ttcgattcaa aaatatgatg ccggccttga
     9301 tatggttaac atggcgatga agatgtgtga agattcgcgt tatacccatg tattaccaag
     9361 ggtgctcttt tatcattctg ctttgctgga tcatcctgat atagaccgtg atgagatgat
     9421 ttttactctt gccaaagctg aaaagctggc aagagaaaga ggtgatttcg aatatagtgc
     9481 gcttt
//
LOCUS       DAOTGZ010000095         9474 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_3064
            NODE_8612_length_9474_cov_5.098843, whole genome shotgun sequence.
ACCESSION   DAOTGZ010000095 DAOTGZ010000000
VERSION     DAOTGZ010000095.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868088
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 9474)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9474)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:22:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,785
            CDSs (total)                      :: 2,751
            Genes (coding)                    :: 2,732
            CDSs (with protein)               :: 2,732
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 12 of 19
            Pseudo Genes (internal stop)      :: 5 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9474
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8612_length_9474_cov_5.098843"
                     /isolate="AMR_MDS_3064"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..694
                     /locus_tag="P5123_07545"
     CDS             <1..694
                     /locus_tag="P5123_07545"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRX16151.1"
                     /translation="GKYVNSTKSYTIEPTYEGGPLDGKQKMEYEDTASGFGGILGVDV
                     RVNNQLNIGLRIESEIALEYERDVKKDFSSTLEDGAKIDRDLPAVIGLGVEYKINEQF
                     LIRPTFQGYMIGWANESKKDAYSEDYDDLGWEAGIGFEYAVMPGFLVASLGYLYSDLG
                     GNEDTYNDGEYALDAHSIGTGCKLTPAPNVDITLGISGTFYTEGESDALGFDETFNKR
                     VINIALGAEYRI"
     gene            865..2082
                     /locus_tag="P5123_07550"
     CDS             865..2082
                     /locus_tag="P5123_07550"
                     /inference="COORDINATES: protein motif:HMM:NF018877.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AGE family epimerase/isomerase"
                     /protein_id="HRX16152.1"
                     /translation="MKTDPNCLLNTLKEVQSSFDHIFDYWMNRAFNRKDNTFFGETDF
                     YGKSKAKADKCIIMYSRILWSFAAAYNHTKNTDYLPFARHCYDFLINSFYDKVNNGFY
                     WSTTFNGIPVIKKKQTYAQAFVLYAFAEYYQSINDIKVLEKAMSLFETIEKRCRDNKA
                     GGYFEAFSENWQILDDVSLSDKDQNDPKGMNTNLHILEAYTRLLQITNDDHVKSSLKS
                     LIEIYIKHIFNDQNHVVLFFDENWNPRSNIISYGHDIEASWLIWDAAKTINDESFCNI
                     ILPFVLKIAKTFKQEGIVNKGAVVYEYFPDSNKTDTDRHWWTQVEAIEGLCNAYRLNQ
                     DQNYLDSALDIWNYTYTYFPDKTNGEWHSVIKEDNTPDTKRIKTGAWKTPYHISRALI
                     RFETFLNKLISDK"
     gene            complement(2154..3056)
                     /locus_tag="P5123_07555"
     CDS             complement(2154..3056)
                     /locus_tag="P5123_07555"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRX16153.1"
                     /translation="MRRSVINRALILLFFLAAPGLVSSSQVPDFLPITVENISRYTED
                     RIAFQHVDVFTDAMMDNSFEVLLLIKKSRMQLIGDGFDDPKIVAERRLIADMQRKLYE
                     DEDMWVNRMNGKLDLMVVTDRRIPVMTSYDEEFVSEKFGGYYTSIRDKFVKRHADIFR
                     QLMRHRKSSELWVERTPLPLPFHLGASKKDKVKYSVVAKAKTTDGRIYTCMDMDGDGV
                     TENFSVTMPDGFNWGYKSGPNIIFIYNNRSEALKKIIGDLATEAYYGTGEEMKEMEET
                     IPKQEEDILDWMETDLIHYEKFYE"
     gene            complement(3230..4480)
                     /locus_tag="P5123_07560"
     CDS             complement(3230..4480)
                     /locus_tag="P5123_07560"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRX16154.1"
                     /translation="MNYTFLEQFILLDALSSPFQGMGRGHIASHFKIIEGFTDTKEAL
                     KDHPEKWRKSGLYTAYSQILFLLACCARKSGLSISDLHDIFEQGSLSPEEWKGIFRYP
                     DRITAAFVERIIRPELPSEGVGGTAAVIYSVLLSLLASKKESSSVEMARRVLLFTTDP
                     CSIVGSIIFEKLVRMSLNRVPNRFASEFTAVCQRCESAFDELLPWVFENGIDPLLVEK
                     SLSYYKKMALILESGEASESSIVSLAGSVTKNSITRATVDMPLAMISFSIYCVDKDRL
                     NGSFFESMLRYGGNVQQLILLTGMLIADTTMLKEEFGNLTDDLVNKTKITAGIKEVAS
                     GRFTDKQFWEFFASERKLSQKCVEELKSICKHGKKSKKPTKPKKTQRMSIEEKLSQHV
                     VSSWTKMDKARYKKEKRHVSVEED"
     gene            4788..5837
                     /locus_tag="P5123_07565"
     CDS             4788..5837
                     /locus_tag="P5123_07565"
                     /inference="COORDINATES: protein motif:HMM:NF016648.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FecR domain-containing protein"
                     /protein_id="HRX16155.1"
                     /translation="MKRISVITLLSLFLVSCTFSKPAGKSAHISFIIGDVTKNGLAVS
                     IHDKVNAGDIIKTSGLSSCDIQIGDSMIRIKENSNLTITLSLIDSHSEQTDLSLDSGK
                     VLCKPKKLLDNESFTVRTPTAVAAVRGTQFSVTTDALKTTRIKVFDGEVKVARRVKTL
                     DNNLEAVIKNGSSIPEEHTAVISEEDALKAADMVDKAIADGQSIEDTISVAPLEVPKK
                     QINTFTPSDFIDEEEIIRIGPKDEDVINKIATAVKKPTGPKGRLLVTRYDAYIIVGGK
                     IIWESKLISPPVKNDDMVIIASSDRIVAAAKDGLILWNRSLKNNGKIRIKNGTLYVGT
                     KKGEKKLLIKNGKIY"
     gene            complement(5847..>9474)
                     /locus_tag="P5123_07570"
     CDS             complement(5847..>9474)
                     /locus_tag="P5123_07570"
                     /inference="COORDINATES: protein motif:HMM:NF020083.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="helicase-related protein"
                     /protein_id="HRX16156.1"
                     /translation="DWLTMVVDKSLEIIKKHKTTLLFANSRRMVEKIARLINERAGEE
                     IAFSHHGSLSREIRSLVEERLKSGELKALVATGSLELGIDIGDVEFVVLIQTPRGVNA
                     AIQRIGRSNHTVDGISEGAFLPTHGRDFLEAAVTAWSVKKGYIEPIHIPLNPLDMLAQ
                     LILSICSVDEWHVEDLYAFIKTIYNYQTLKKTFFEIVVEMLCGRYAGLRVRELSAKLN
                     YDRIDGTVRSRDGIRFQLYSSGGAIPDRGLYNMKLQSNGYRIGTLDEEFVWERNIGDT
                     FALGVQCWVIKKITHNDVEVAPAVNRPGIIPFWRAEQESRHFSLSKRLLELLKECELI
                     LGTDHNLPDNLFIEKYQLSRDAASRLADYIARQRDITGCALPNIENIVVEHCSDPENT
                     TDNKQVIIHTMWGRCINMPAAYAISSWWESEYGYPLEVFINNDAILLQLPHKCNGREL
                     FDAIINSDIQKLIRRHIEKTGLFGSVFRENASRALLLPPKGFKNRQPLWLNRLRAKKI
                     LESVKDYSDFPVMIETWRTLLCDIFDMDNLALLLDKIEAGEIKISECITRAPSPFCDD
                     LVWNQVNKYMYQDDTPSGGIASSLSDSLIRDITRGFLSRVAIDRNTIEILDSRLKRTF
                     EGYAAFNTADLIGWIDERVVVTQEEWHSLLAARERDRKKDEVLDMDKISCVIAKFRIN
                     NTVVYSYVQNCAIIKRYFPDICGTFESIDEKNSDIIVQRISKIMQKSNNIDFSSLFKQ
                     FLSYYAVFDLELVKMLFADKGVDILNTLLADNSVISDRLCEESSETFFTVRENYEILL
                     RMQRRSRRPGLTALPVKYLQDFLAEYQNLGSGKTVEDLQNSIEQLCGLPLESQLWEEA
                     VFTARIADYTPLWLDSLISSTDLICCGAGKAKFTFIFNEECPVFKKGSSSDESDEYFG
                     SRPFSFSEFASRFALTAHEGLERLYSAFFRGKLICTGFDHIRKATINRFKTDVLSSSL
                     GRVSFGRWKSSQPTDSLWRALVSDPVESEFENLELEKERARQLLSRYGVIFRQLTDKE
                     DKPLRWAAVFRALRLMELSGEIVSGYFFEGVDSLQFVSQEGLRFFTEFSGKKNRELIY
                     WFNAKDPASLAGCDIDYYKENMPRRIASNYVVYGRGKPVLVCEKNFSRLRFLLSPEES
                     EKEHLKIFRDILGRRFNPPSKIRVDFINGEPAHISEYSSLLISAGFRKDYRSLVLERN
                     SGYK"
BASE COUNT         3089 a         2067 c         1610 g         2708 t
ORIGIN      
        1 gggcaagtat gtcaactcaa caaagagtta cactattgaa ccaacatatg aaggcggccc
       61 acttgatggc aagcaaaaga tggagtatga agatacagca tcaggtttcg gaggcatact
      121 tggtgtagat gttcgtgtaa ataaccagct aaacatcgga ctgcgtattg agtcagaaat
      181 cgcactcgaa tatgaacgtg atgttaaaaa ggatttttct agcactcttg aagatggtgc
      241 aaaaatagat cgcgatcttc cggcagtaat tggtcttgga gtggaataca agattaacga
      301 acaatttctg atacgcccaa catttcaggg atacatgata ggctgggcaa acgagtctaa
      361 aaaagacgca tacagcgaag actatgacga tttaggttgg gaagccggta ttggctttga
      421 atatgcagtg atgcctggtt ttcttgtagc atcactcgga tacctctact cagatttagg
      481 cggtaacgaa gacacctaca acgatgggga atatgcactt gacgctcact ctatcggaac
      541 cggatgcaag ttaactcctg caccaaatgt tgatattaca cttggaattt caggaacctt
      601 ttacactgaa ggcgaatcag atgccctcgg tttcgatgaa acattcaaca agcgtgttat
      661 caatattgct cttggcgcgg aatacagaat ctgataataa acaaaatcgc acttttaaag
      721 gatcgcttaa cagcggtcct ttttttatcc aaacagatca gaaatatttt tgtaagcagc
      781 aataactgct atataaaata ttgttgaatt aatcccttaa aatgaatata ctgccaaaaa
      841 ctccgcgaga taacgttaac tgacatgaaa acagatccaa actgcttatt aaatacactc
      901 aaagaagtcc aatcatcttt tgatcatatc tttgattact ggatgaacag ggcatttaac
      961 cggaaagata atacattttt cggtgaaact gatttttatg ggaaaagcaa ggcaaaagct
     1021 gacaaatgca taattatgta cagccgaatt ctatggtcgt ttgccgctgc ctacaatcat
     1081 accaaaaata cggactacct acctttcgca cgacattgtt acgatttttt aataaattct
     1141 ttttatgaca aagtaaataa cggtttctac tggagcacaa cgttcaatgg catcccagtt
     1201 attaaaaaga aacagaccta cgcccaggcc tttgttctat acgctttcgc ggaatattat
     1261 caaagcatca atgatattaa ggttttagaa aaagccatga gtctcttcga aaccatagaa
     1321 aaacggtgcc gagacaataa agcagggggc tattttgaag cattttcgga aaactggcaa
     1381 atattggatg atgtaagcct aagtgataaa gaccaaaacg accccaaagg catgaacaca
     1441 aaccttcaca ttctggaagc ctacacccgt cttctacaga ttacaaatga tgaccatgtg
     1501 aaatcctcct taaagtcact tattgaaatt tacattaaac atatttttaa cgatcaaaat
     1561 catgtcgtcc tcttttttga tgagaactgg aatcctcgaa gcaatatcat atcctacggt
     1621 catgatatcg aagcatcatg gcttatctgg gatgccgcaa aaacaatcaa cgatgaaagc
     1681 ttctgcaata taatcttacc gttcgtgtta aaaatcgcaa aaaccttcaa acaggaaggc
     1741 attgtcaata aaggcgcggt tgtttatgag tatttccccg attcgaataa aacagacaca
     1801 gatcgtcact ggtggacaca ggtagaagcc atcgaaggtc tgtgtaacgc ctaccgactt
     1861 aaccaagatc aaaactacct tgattcggca cttgatatat ggaattacac ctatacatat
     1921 tttccagaca aaacaaacgg tgaatggcat tcagtcataa aagaggacaa tactccagac
     1981 acaaaacgca taaaaacagg agcatggaag accccatatc atatttcaag agcacttata
     2041 agatttgaaa ctttccttaa taagcttata tcagataaat aacaatttat atacaacaag
     2101 agttccctaa ggaactcttg ttgtattgta tctgcaaatc acttttgtgt tacttattcg
     2161 taaaactttt cgtaatggat aagatcagtc tccatccagt caagaatatc ttcttcctgt
     2221 tttggaatgg tctcctccat ctccttcatc tcttcaccgg ttccataata cgcttccgta
     2281 gcaaggtcac caattatttt tttaagagct tcggaacgat tattataaat aaaaattatg
     2341 ttcggaccag acttgtatcc ccagttaaac ccatcgggca ttgtaacaga aaagttttct
     2401 gttacaccat caccgtccat atccatacat gtatatattc gcccatctgt cgtcttggct
     2461 ttagccacaa ccgaatactt aactttatct ttcttggatg cccccagatg aaaaggcaaa
     2521 ggcaaaggag ttctctctac ccatagctct gacgacttgc gatgacgcat aagctgacga
     2581 aaaatatctg catgccgttt cacaaattta tccctgatag atgtataata cccgccaaac
     2641 ttctcggaca caaattcttc atcatagctt gtcataaccg gaatacgacg atcagttaca
     2701 accataagat caagttttcc gttcattcgg ttaacccaca tatcttcatc ttcataaagc
     2761 tttctttgca tatcagcaat caaacgacgc tcagctacta tttttggatc atcaaaacca
     2821 tcaccgatca gttgcatacg actttttttt atcagtaaaa gcacctcaaa agagttgtcc
     2881 atcattgcat cagtaaagac atcgacatgc tgaaaagcta tacgatcctc tgtgtaacgc
     2941 gaaatattct ccactgtaat cggcaaaaaa tcaggtacct gagatgaact gactaaaccc
     3001 ggagcagcca gaaaaaacag cagtataaga gctctattta ttacagatct tcgcatctaa
     3061 atcctccgtg tgcaatgttt cacaatatag tagggtaaaa atccatttcc acctatagat
     3121 cgtatcgtca caaaaggtat atataatgag cctgtttgtc aaatattttt cctttggaca
     3181 tttttttgcg taaagaacat ttaataatta gctatcttta gatcaataat caatcctctt
     3241 caaccgaaac atgacgcttc tcttttttat atcgagcctt atccatcttt gtccaactgc
     3301 ttaccacatg ttgtgacaac ttctcttcaa tgctcattct ttgcgttttc tttggtttag
     3361 tcggtttttt actcttttta ccatgcttac aaatcgattt aagttcttcc acgcattttt
     3421 gactcaattt gcgttccgag gcaaaaaatt cccaaaattg tttatctgtg aatctaccag
     3481 aagcgacttc ttttatacca gcagttattt ttgtcttatt gacaagatca tccgtaagat
     3541 ttccgaactc ttcctttaac attgtagtat ctgcaataag cattcctgtt aaaaggatca
     3601 attgttgcac attccccccg taacgtaaca tcgactcaaa aaaagaaccg ttcaaccgat
     3661 ctttatcaac acaatatata gaaaaggaaa tcatggctag cggcatatcg acggttgcac
     3721 gtgttatact gtttttagtg acacttccgg ctaaagaaac aatactactc tcagaggctt
     3781 caccgctttc aagaataaga gccatttttt tataataaga taaacttttt tccactaata
     3841 gcggatcaat accattttca aaaacccatg gcaaaagctc atcaaatgca gattcacacc
     3901 tctggcaaac agccgtgaac tctgaagcaa acctgtttgg tactctatta agtgacatgc
     3961 gaaccagctt ctcaaaaata attgagccaa ctattgaaca aggatcagtt gtaaacaata
     4021 gtacccgtcg agccatttca actgaagaag actctttttt tgacgccagc aaagataaaa
     4081 gtaccgaata tatgacagcc gcagtaccac ctactccttc tgatggcaac tcaggacgaa
     4141 taatcctttc aacaaatgca gctgttatcc gatcaggata acgaaatatc cctttccact
     4201 cttccggcga tagtgaaccc tgttcaaaga tatcatgcaa atcagaaatt gagagccccg
     4261 atttccgagc acaacatgct agcagaaata gtatctgcga ataagccgta tacaaaccgc
     4321 tttttctcca tttttctgga tgatctttaa gagcctcttt tgtatcggta aaaccctcaa
     4381 taattttgaa atgcgaagca atgtgacctc gccccatacc ctgaaacgga gaagaaagcg
     4441 catcaagaag tataaactgc tctaaaaaag tataattcat cagaattcac ctctacaatt
     4501 tataaaatgt tttcatgact accgttttgc agattattat acaattaatt aaggttttta
     4561 agtaatcaaa aataattaat tgaaaatact ctgtaattat tgtacattaa caacaatgtt
     4621 ccttatataa atggtctgta tatacatgaa agtgtctact tccatacaaa aaagtttaaa
     4681 caaatcatat tttactttat taaatccgca tttttatgta caaaagatct tcttaaaact
     4741 gttcagtcaa tgatgaatta agaatcagcc aatattacga ggataccatg aaaagaatat
     4801 ctgttataac attattatcc ctatttttag tttcctgcac attctcaaaa ccggcaggaa
     4861 aaagcgctca tatcagtttc attattggcg atgttactaa aaacggatta gcagtttcca
     4921 tacatgataa ggtaaacgcc ggtgatatta taaagacttc gggactctcg tcatgtgata
     4981 ttcaaattgg agattcgatg ataagaatca aggaaaacag taatttaacc attaccctgt
     5041 cccttattga ttcccacagc gagcaaactg atctttcact tgatagcggt aaagtcctct
     5101 gcaaaccaaa aaaattatta gataacgaat catttacggt acgcacacct acagcagtag
     5161 ccgcagtaag gggtacacaa ttttcggtaa caacagacgc tcttaaaaca acccggatca
     5221 aggtttttga cggagaagtc aaggttgccc ggcgcgtcaa gacacttgac aacaacctgg
     5281 aagctgtaat taaaaacggg agttctatac cagaggagca cacagctgtt atctccgagg
     5341 aagatgcgtt aaaggctgcc gacatggttg acaaagcaat cgctgatggg caatcaatag
     5401 aagatacaat ctcagttgcc ccattagaag ttcccaaaaa acagatcaat acctttaccc
     5461 cttcggactt cattgacgag gaggagataa tccgaatcgg tccaaaagac gaagacgtta
     5521 tcaataaaat tgcaacagcg gttaaaaaac caactggtcc caaaggacgg cttcttgtca
     5581 caagatacga tgcctatata attgttggcg gcaaaattat atgggaatca aaactcatat
     5641 cgccacctgt aaaaaacgat gacatggtca taatagcatc atcagaccga atagtggccg
     5701 ccgcaaaaga cggattaata ttatggaaca ggtctctaaa aaataatgga aaaatcagga
     5761 taaaaaatgg gacactgtat gttggaacaa aaaaaggtga aaagaaactg ttaataaaaa
     5821 acggtaaaat atattaaaga ttctctttat ttataaccag aattccgctc cagaaccagg
     5881 gatctgtagt cttttctaaa accggcgcta atcagtaaac tgctgtattc agatatatgc
     5941 gccggttctc cgtttataaa atcaaccctg attttagaag gcgggttgaa gcgtcttccc
     6001 agaatatccc tgaaaatctt aaggtgctct ttttcagatt cttccggact cagaaggaac
     6061 cttagtcgcg agaaattctt ttcacagaca agcaccggtt taccacggcc atatacaaca
     6121 taatttgaag caatccttct aggcatattt tccttgtaat aatcgatatc acagccggca
     6181 agactggccg gatccttggc attgaaccaa taaattaact ctctgttttt tttacccgaa
     6241 aattccgtaa agaatcgaag cccttcctga gaaacgaact gaagagaatc aactccctca
     6301 aaaaaatatc ctgacactat ttcaccagac aactccatca atcgcaatgc tctaaaaacc
     6361 gctgcccatc ttaaaggttt atcttctttg tctgttaact gacgaaaaat cacaccataa
     6421 cggcttaaaa gctgccgcgc gcgctctttt tctagttcaa gattctcaaa ttcagactct
     6481 accggatccg acacgagcgc acgccaaagt gaatcggtcg gctgactact tttccagcgt
     6541 ccaaatgata ctctacctaa actactggat aaaacatctg ttttgaaacg gtttattgtt
     6601 gcttttcgta tatgatcaaa ccctgtacag attaatttcc ctctaaaaaa agcggaataa
     6661 agtcgctcaa gaccctcatg agcagttaaa gcaaaacgtg atgcaaattc tgaaaaagag
     6721 aatggtcttg atccaaaata ttcatcactc tcatctgaac ttgatccttt cttgaaaaca
     6781 ggacactctt cattaaatat aaaagtaaac ttagcttttc ccgcaccaca acagataagg
     6841 tctgttgaag atatcagcga atcaagccaa agcggtgtat aatcggctat tctagctgta
     6901 aaaacggcct cctcccataa ttgtgactca agaggcaagc cacacaactg ttcaatcgaa
     6961 ttctgtaaat cttcaacggt ttttcctgat cccagatttt gatactctgc tagaaaatcc
     7021 tgcaaatatt tcactggaag agcagttaac cccgggcgac gacttctgcg ttgcattcga
     7081 agcaatatct catagttttc tcttacagta aaaaaagttt ctgaagactc ttcacacaat
     7141 cgatcactta tcacactgtt atcagccaac aaggtattca ggatatccac tcccttgtca
     7201 gcaaaaagca ttttaaccaa ttccaaatca aaaacagcgt agtaagataa gaactgtttg
     7261 aaaagagagc taaaatctat attattcgat ttttgcatta tttttgatat tctttgaaca
     7321 attatatcgc tgtttttttc atcaattgat tcaaatgttc cacagatatc cggaaaatat
     7381 cttttaatta ttgcgcaatt ttgaacataa gagtacacaa ccgtattatt aattctaaat
     7441 ttagctatca cacatgagat cttgtccata tccagaactt catctttttt acggtctctc
     7501 tctcttgccg cgagaagaga gtgccactcc tcttgcgtaa caacgacacg ttcatcaatc
     7561 cagcctatta aatcagcagt attgaaagct gcatatccct caaaagtccg tttcaatctt
     7621 gagtcaagaa tttctattgt gttcctgtca attgcaaccc gagacaaaaa tccccgagta
     7681 atgtcgcgaa taagtgaatc actcaatgag cttgcgatac cacccgacgg agtatcatcc
     7741 tgatacatat atttattaac ctgattccag accagatcat cacaaaatgg agatggagca
     7801 cgggtaatgc attcagatat ttttatctct cctgcttcga tcttgtcaag aagcagcgca
     7861 agattatcca tatcaaaaat gtcacataat aaggttcgcc aagtctcaat cattacagga
     7921 aaatcagaat aatcttttac agattctagt attttttttg cacgtaaacg attaagccaa
     7981 agaggctgcc ggtttttgaa accctttggg ggcaacaaaa gcgctcgtga agcattttca
     8041 cggaagaccg agccgaagag tcctgttttt tcaatatgcc ttctaatcag tttctgtata
     8101 tcactattga taatagcatc aaacaattca cgcccattgc acttatgcgg aagctgcagt
     8161 aatattgcat cattgtttat aaagacttca agaggatatc catattcaga ttcccaccag
     8221 gatgagatag cataagccgc tggcatattg atacagcgtc cccacattgt atgtataata
     8281 acctgcttgt tatcagttgt gttttcagga tcactgcagt gttcaactac aatattttcg
     8341 atattaggca aagcgcagcc tgttatgtcc cgctgtcttg ctatataatc cgccaaccgt
     8401 gatgccgcat cacggcttag ctgatatttt tctataaata aattatcagg taaattatga
     8461 tcagtaccaa gtattaattc acactctttc aataactcca gaagcctttt gcttaacgaa
     8521 aaatggcggc tttcctgttc ggcccgccaa aacggaataa ttcccggacg gtttacagcc
     8581 ggtgcgacct caacatcgtt gtgcgtaatt ttttttatca cccagcattg tacacctaaa
     8641 gcaaatgtat ctccaatatt acgctcccaa acgaactctt catctaacgt accaatcctg
     8701 taaccgtttg attgtaattt catattatac agaccacgat ccggtattgc acctccgcta
     8761 ctgtaaagct gaaaccttat accatcgcgc gagcggacag ttccatctat acgatcataa
     8821 tttaattttg cggataattc cctaacacgc aatccggcgt acctgccgca aagcatctca
     8881 acaacaattt caaaaaatgt tttttttaga gtctggtaat tataaatagt ttttataaac
     8941 gcatacaagt cctcaacatg ccactcatcc acagaacaaa tagaaagtat aagctgtgcc
     9001 agcatatcta acggattaag aggaatatgg ataggttcta tatatccttt tttaacagac
     9061 catgcagtta cggcagcttc aagaaaatca cgtccgtgtg tcggcaagaa agcaccttcg
     9121 ctgataccat ccacagtatg attcgagcgc ccgatacgtt gtatggcagc atttacacct
     9181 ctgggtgtct gaataagtac tacaaactca acatcaccaa tatcaatccc cagctcaagg
     9241 gaaccagtgg ccaccaatgc tttaagttcc ccgcttttaa gacgctcttc aactaacgaa
     9301 cgaatttcac gggaaagcga accgtgatgc gaaaaagcta tctcttcacc ggctcgttca
     9361 tttattaaac gagcaatctt ctcgaccatt cttctgctat tggcaaaaag cagagtagtc
     9421 ttatgttttt tgattatctc aagggattta tcgacaacca tggtcagcca gtcc
//
LOCUS       DAOTGZ010000096         9457 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_3064
            NODE_8633_length_9457_cov_4.514252, whole genome shotgun sequence.
ACCESSION   DAOTGZ010000096 DAOTGZ010000000
VERSION     DAOTGZ010000096.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868088
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 9457)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9457)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:22:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,785
            CDSs (total)                      :: 2,751
            Genes (coding)                    :: 2,732
            CDSs (with protein)               :: 2,732
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 12 of 19
            Pseudo Genes (internal stop)      :: 5 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9457
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8633_length_9457_cov_4.514252"
                     /isolate="AMR_MDS_3064"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..618)
                     /locus_tag="P5123_07575"
     CDS             complement(<1..618)
                     /locus_tag="P5123_07575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003545200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0043039 - tRNA aminoacylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate--tRNA ligase family protein"
                     /protein_id="HRX16157.1"
                     /translation="METVTSNFIEEIINDDLKSGKNSCVVTRFPPEPNGYLHIGHAKS
                     ICLNFGLAKKYNGRCHLRFDDTNPSKEEDEYINSIQEDVRWLGFDWGEHLHFASDYFD
                     KLYDFAILLIKEEKAYVCDLSADETREYRGTLKEAGKESPYRSRSVEENLELFSNMRA
                     GKYKEGEKVLRAKIDMKSGNICMRDPIIYRIQYSHHHRTGDKWCIY"
     gene            912..2636
                     /locus_tag="P5123_07580"
     CDS             912..2636
                     /locus_tag="P5123_07580"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HRX16158.1"
                     /translation="MKKNHSTDFMDLERSFMSLVKRIESATEKLSLMKRPAPENLDEW
                     IKELGLIKKHFSSEILRQKEFCSTLFESEMKYRELFESSIDYLFLVQAGNMQILDINK
                     SAKKRFGDVTDFKELFEFEGENFGSSVKRYFDQVVEEGETCVKASYHRNDSTKVYVSL
                     RMQRLDLQGKIVILVSVRDLSEELDTEARLIQSQKLETLGMLAGGIAHDFNNILSGIT
                     GTVSLMKYQLDNGDKISDDDLYQYVEVMQGSSDRAREIIQQLLAFSKNNSIGKGQVEV
                     NQALDTVANVCKNTFDKSININFNRFDREIYIAGDPAKLDQILLNICINASHAMTIMR
                     ANDEPYGGVLKLECEVKVENKQEFAVIRISDTGIGMDEDTKKQLFDPFFTTKDSDSGT
                     GLGLSMVYAMTTKMNGLIAVDSSPGNGAVFTLQFPTVKNIKTSTGVQHRGRAALKGTV
                     LLVDDEDMLRATTSRMLKNFGLSVIEACNGQECIDIFIQNNRDIDLVLLDIMMPGIGG
                     DEVFMVINKINPKVPVIMVSGFVQKDRFERINKKNIAGFISKPFTLDELYSSIGGVLN
                     HRDGSISS"
     gene            complement(2622..3581)
                     /locus_tag="P5123_07585"
     CDS             complement(2622..3581)
                     /locus_tag="P5123_07585"
                     /inference="COORDINATES: protein motif:HMM:NF013255.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HRX16159.1"
                     /translation="MKIFVTGGTGFIGRNLIPRLVQKRHKVTAVARSAYGKSQLDLMG
                     ATPVDGNLASVSTFTSNFSKADAVIHLGAKTESWGLYNEFFEINVRASEEITGAASYH
                     GVKKLIYLSCASVVCTGNPLFDVDEDYKPSGFPSDNYSKSKAIAEEKVRNASSRLNTV
                     ILRPAFVWGKQVPFIERLRHHINRIGFPNIGKQGHQFSTCHVENIVEAIITALDQPDK
                     SGTFYVTDGEPVAVKFFMPQLLLSHGLFAGRVNLPRSFALVLAGFFEIIWAIIPFKKT
                     PPLTKNIVNILGSEATFSDNKFREVFSFKNRVQPEEELRSRRY"
     gene            complement(3723..4895)
                     /locus_tag="P5123_07590"
     CDS             complement(3723..4895)
                     /locus_tag="P5123_07590"
                     /inference="COORDINATES: protein motif:HMM:NF012376.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HRX16160.1"
                     /translation="MRTGSFIIFLSIFFSIYSLSNIFIYFVIRKLFNLKGIKRLILKI
                     SHILIASLYISSRFIENYWRNSLTESLAAIGSFYLAVMLYLFLLSLTGVIIWFILKRF
                     SNAAILTNKHKDKTLKYAGIFMVMFVSVITIYGYHHACSPQVKYHKISISKSQFSSPL
                     RIAFVSDIHLGTIIKNKRLNKMVTSVNSFSPDIILLGGDIVDEDIAPVIKYDMGALLT
                     KLNAPLGVFGVTGNHEYIGGVEKAVKYLEDHNITMLRDKAIIIPGRLILIGREDFSIT
                     RFTSKARLPLSEIINNQISPSDWTQLPSIIVDHQPYGINEAITQKADFMMSGHTHNGQ
                     LWPIKYIVSQIFKIRHGLKKFEDTWFLVSSGYGTWGPPVRVATDSEIIIIDLFPEN"
     gene            complement(4952..6418)
                     /locus_tag="P5123_07595"
     CDS             complement(4952..6418)
                     /locus_tag="P5123_07595"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRX16161.1"
                     /translation="MQTSAKSVVKHKIISTQCQSCGHTFNLSSEKINSFQNGMTHCPS
                     CSNPIKITFCPECNISYSISTANLKPNRYATKCKQCGYGFYIDIPEDPEQKHKMPFIT
                     RVTEPIEKAPVLQKPVSTSKPETKKTESVNSSEVIKKIEPPRQQNLQETSLSEVFKLI
                     SKAFTLRNFSLSFISIISMISIFFISTNIITFLTPPPQASGNYLLSFLNIIPPLLVFA
                     VYTAFASVSAKRLLITNGFQLFNNTAGLKLFANSLLASLFGGAPALIAIQTLLVFITR
                     IPDGGTLIFALTFLPIYIGALLTVLTLTIGFWFLPSIYAAKKRTVLGYLHEFIYFLRK
                     HNINLIINVLTLFMLAGALSAAIFFAHATALSLAETIMSGVGNSNITQTLSNIPVLFT
                     SFAKFSFFGENSSFLNYLTAQTSLFSTTGGFIIGICFTLITLVLYSLILTVLTASGTA
                     IYSQLEHPSSGSNLIPGKGIMIMLILFYFAVLAMKAIF"
     gene            complement(6441..8678)
                     /locus_tag="P5123_07600"
     CDS             complement(6441..8678)
                     /locus_tag="P5123_07600"
                     /inference="COORDINATES: protein motif:HMM:TIGR02074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PBP1A family penicillin-binding protein"
                     /protein_id="HRX16162.1"
                     /translation="MHKRFSIILLLVTLLLTPYFLFVYMYHSQYDLISSDLEYTTNLP
                     TTLFDSNGTIIAELYDEKRSPVKFEQIPDTVITAFTSAEDKNFFKHRGLDFVGIMRAL
                     IVDIKQGSIKQGGSTITQQLIKQLYTHKKRTIERKIIEILLIKKIEENFTKNQILEMY
                     LNQIYFGHGVYGIKSAADFFFGKELKNIDIYEASLLALIPPAPNRYSPLRNPRITFSK
                     HKQIIVNLVQNGHISKKDALYNFEKFWSRYLEQIIDRSPNEIARNKNDDKAPYLTDYI
                     RQQLIEKYGEDIVYKGGLKVYTTFDLKMQQIAQELLTERITQQQKISSKYNDRELAIL
                     ENDFYISKEQKISINEITALKDIRSQISDETSFLALICGADKITDIFSNYSRFYRQQR
                     QLANVEGAFTAIEPDSGKIKVLVGGKKFSYENQLNRGVNSRRQPGSAFKPFVYASGIE
                     DKIITAATQFQDLPMVFNERTKTWAPSNASDKFSGPVLVRKAITRSINTIAVLAYEKI
                     GGDRIASYASRSIHIGKNRFTIDPTLGLGSSELTPLELTTGFACIANQGIPVKPYAIQ
                     KILDNHNKEIYVHEPKTQKAVFSRGTAFIMNSILQAVITNGTATSTIRNRCGFTSPAA
                     GKTGTNSDYRDAWFAGYTPDLAAAVWIGCDSQKFTLGPGQYGANVAAPVWANFMKETA
                     SIRRKLAFPPKPPEIIQISVCEHSGDLPSEGCPVIQEYFLEGTAPKKRCSSKHESMKS
                     IYDLF"
     gene            8889..>9457
                     /locus_tag="P5123_07605"
     CDS             8889..>9457
                     /locus_tag="P5123_07605"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRX16163.1"
                     /translation="MKMSVRGELVLVWFFLGYGLYFFTGIYTFLPMMIFFAPIFILHL
                     LEKQELFEAWIFSFIGFVLAVQLSTLPFSDYEGVNWLYSGVLKNFIIAAGIALPYWLT
                     RLVYHKNSGFAHTFSFPIFTTSVYFILSKVNFFNGLSGFSHYLQADNEWLLQFVSLSG
                     IWITVFFISWTASIACWIWNNSVNLGRVGG"
BASE COUNT         2854 a         1499 c         1909 g         3195 t
ORIGIN      
        1 gtagatgcac catttatcgc ctgtgcgatg gtgatgtgag tattgtatcc ggtaaataat
       61 aggatcacgc atacagatat ttccagattt catatctatc tttgctcgca aaactttttc
      121 accctctttg tatttacctg ctctcatatt tgaaaaaagt tcaaggtttt cttcaactga
      181 tctgcttcgg tacgggctct ctttgcccgc ttctttaaga gtgcctcggt actctcttgt
      241 ttcatcagcg ctaagatcgc aaacgtaggc tttctcctct tttataagga ggatcgcgaa
      301 atcatataat ttatcgaaat agtcagaggc aaagtgcaga tgttcacccc agtcaaaacc
      361 aagccagcga acatcttctt gaattgagtt gatatactcg tcttcttcct tacttggatt
      421 tgtatcatca aaacgcaagt ggcatctgcc gttatatttt ttggcaaggc cgaaatttag
      481 gcatattgat tttgcgtgac caatatgtag atagccgtta ggttctggcg ggaaacgggt
      541 aaccacgcaa ctgtttttac ctgattttag atcgtcgttg attatttctt caataaaatt
      601 tgatgttact gtttccatat agtctccttt tagcataata acgtaaggat aatcaatgat
      661 accctatctt gtggagtgat aaacctgtca aatactatta ggtgtgattt tgcttgcaag
      721 tttatctttt aacaggtttg aaaaatatgt gtatttcata aattaacagt tcaataaaaa
      781 agatttgcat aacttacaaa ttgctgctat ctttgaatta aataaaagtt agcttctatt
      841 taattgttgc taattgtcag aaaatgatat gagataagcc cgatttaatt tgctaaaagg
      901 atatataata ggtgaaaaaa aatcacagta cagacttcat ggatttggaa agatcattta
      961 tgtcactggt caagcgaatt gaatcagcaa ctgagaaact atcacttatg aagcggccag
     1021 ctcctgaaaa tctggatgag tggattaaag agcttgggtt aattaaaaaa cacttttcta
     1081 gtgaaatatt gcgtcaaaaa gagttttgct caacgctttt cgagtccgag atgaaatacc
     1141 gtgaactttt tgaatcttcc atagattatc tttttttagt gcaagctggt aatatgcaaa
     1201 ttcttgatat taacaagagt gcgaaaaaaa ggtttggcga tgttaccgat ttcaaggaac
     1261 tgtttgaatt tgaaggtgaa aacttcgggt cgtctgttaa acgttatttt gatcaggttg
     1321 tcgaagaggg cgaaacatgc gttaaggctt catatcacag gaacgatagt accaaagttt
     1381 atgtatcttt aagaatgcaa cgactcgatt tgcagggtaa aatagttatt cttgtatctg
     1441 tgcgtgatct ttctgaagag ctggatacgg aagcccggct gatacaatca caaaaactgg
     1501 aaactcttgg tatgctggca ggtggcatag cccacgattt taataatatt ttaagtggca
     1561 taactggaac agttagcctt atgaaatatc agcttgataa tggagacaaa attagtgatg
     1621 acgatttata tcagtatgtg gaagttatgc agggttcatc ggatcgcgcc cgtgaaatta
     1681 tacaacagct gttggcattc tctaagaata acagtattgg aaagggacaa gtagaagtta
     1741 atcaggctct tgatacggtt gcaaatgtat gtaaaaatac ttttgataaa tcaataaata
     1801 ttaattttaa tagatttgat agagaaatat atattgccgg agatcctgca aaactggatc
     1861 aaatactgct gaacatctgt ataaacgctt ctcatgcaat gacaataatg cgtgcaaatg
     1921 acgagcccta tggcggtgta cttaaacttg aatgtgaggt aaaggtcgaa aataaacagg
     1981 agtttgctgt tatacgaata tctgatacag gtattggtat ggatgaagat acaaaaaaac
     2041 aacttttcga tcccttcttt acaactaagg attcggactc aggaactggt ttggggctct
     2101 caatggttta tgctatgaca accaaaatga atggtttgat tgctgtggat tcttctccgg
     2161 gtaatggggc tgtttttact ttgcaatttc cgactgtgaa aaatataaaa acttctaccg
     2221 gagtacagca tcgtggcagg gcagctctta agggaacagt acttcttgtt gatgacgaag
     2281 atatgctaag agccacaact tcaagaatgc taaaaaattt cggattatcg gttatcgagg
     2341 cgtgtaatgg acaagaatgt atagatattt ttattcaaaa taaccgggat atcgatctgg
     2401 tgttactaga catcatgatg ccagggattg gcggagatga ggtttttatg gttattaata
     2461 agattaatcc taaagtccct gtgattatgg ttagtggctt tgttcagaag gatagattcg
     2521 agagaataaa taaaaaaaat atcgcgggtt ttatatctaa gccatttact cttgatgagt
     2581 tatatagttc tattggcgga gtgcttaatc atagagatgg ttcaatatct tcttgatcga
     2641 agttcttcct ctggttgcac cctgttttta aaagaaaaaa cttctctgaa tttgttgtcg
     2701 ctaaaagttg cttcgcttcc tagaatattg actatgttct ttgttaatgg cggtgttttt
     2761 ttaaagggaa ttatagccca tatgatttca aaaaatcctg ctaaaacaag tgcaaatgag
     2821 cgtggaaggt ttacgcggcc tgcaaatagt ccatgagata gtagaagttg tggcatgaag
     2881 aatttcacag caacaggttc tccatcggtt acataaaaag taccactttt atctggttga
     2941 tcaagtgctg ttattatggc ttccacaatg ttttcgacat ggcaggtcga aaactgatga
     3001 ccctgtttgc caatgttagg aaaaccgatt cggttaatat gatgcctgag cctttcaata
     3061 aatggaacct gtttacccca gacaaaggcg ggtcttaaaa ttacagtgtt taatcggctt
     3121 gaggcattgc gaactttttc ttcagcaatg gcttttgatt tgctatagtt atccgaagga
     3181 aatccagaag gcttatagtc ttcatctaca tcaaataatg ggttacctgt gcagactacg
     3241 gaggcacagc ttaagtaaat aagttttttt actccatggt aggatgctgc accggtgatt
     3301 tcttcggacg cccgaacatt gatttcgaaa aattcgttat acaatcccca gctttcagtt
     3361 tttgcaccta gatgaattac tgcatctgct ttactgaagt tactggtgaa tgtgctcact
     3421 gatgcaagat ttccgtcgac cggtgtagct cccataagat caagttggct tttcccatac
     3481 gcggatctgg ctacagctgt gactttatgt cttttttgaa caagtcttgg aataaggttt
     3541 ctgccaatga aaccagttcc tccggtaaca aaaatcttca tatgaatttt tccaaatagg
     3601 tatattgtgc taaattactg ttattcgtta tgtgcaaaga cactttgaaa ttttataaca
     3661 gttaaattat aaactatgct ttaatatgta tatttgtaac agatttcatg tcaagaatta
     3721 tgctaatttt cagggaaaag gtctattatg attatctcgg aatctgttgc aacccgcaca
     3781 ggtggacccc aagtgccgta accacttgaa accagaaacc aggtgtcttc aaattttttt
     3841 aaaccatggc gaattttaaa aatttgagat acaatatatt taataggcca tagctgtcca
     3901 ttatgtgtat gaccgctcat cataaagtcg gccttttgcg tgatggcttc gttaataccg
     3961 taaggttggt gatcgacgat tattgatggt agttgcgtcc aatcggaagg agaaatttgg
     4021 ttattaataa tttcggacag tggcagcctt gcttttgagg taaatcttgt aatactgaag
     4081 tcctcacgcc cgataagtat taaacgtcca ggaataatga tcgctttgtc tcgaagcatt
     4141 gtgatgttat gatcttcaag atactttacc gctttttcaa cccctccgat atattcatgg
     4201 ttgccggtta cgccaaatac accaagtgga gcattaagct ttgttaataa cgcacccata
     4261 tcgtatttta taaccggggc gatatcttca tcaacaatgt ctcctcccaa aagaatgata
     4321 tctggactaa aactgttaac gctagttacc attttattaa ggcgtttgtt ttttataatt
     4381 gtacccaagt gaatatcaga tacaaacgca attcttaaag gtgatgaaaa ttgtgatttt
     4441 gaaattgaaa ttttatgata tttaacctgt ggtgagcatg catgatgata accgtaaata
     4501 gtaattactg atacaaacat aaccataaat ataccagcat atttcaaagt tttgtcttta
     4561 tgtttgttgg ttaatatcgc agcgtttgaa aatcttttta aaataaacca gattatgaca
     4621 cccgttaaag atagtagaaa aagataaagc ataaccgcta aataaaaaga gccaatagct
     4681 gcaagagatt ctgttaggct gtttcgccaa tagttttcga taaatctgga ggagatatat
     4741 agtgaagcga ttaaaatatg tgatattttt aaaatgagtc tttttatacc ttttaagttg
     4801 aaaagtttac gaattacaaa atagataaag atattagata atgaataaat tgaaaaaaag
     4861 attgatagaa aaataataaa cgagcctgtt cgcataaaaa ccgtccttgc agggttttaa
     4921 tctgttacta gtcattctta aagataatca tttaaaaaat agccttcatg gctaaaactg
     4981 caaaataaaa tagaatgagc attatcataa ttccttttcc gggaattaaa tttgagccgg
     5041 aagatggatg ttccagttgt gagtaaattg ctgtaccgga tgcagttaat acggtaagga
     5101 ttaaactgta aagcactaat gttattaaag taaagcaaat accaatgata aaaccaccgg
     5161 tagttgaaaa tagtgatgtt tgggcagtca gatagtttag aaaagaactg ttttctccga
     5221 agaatgaaaa ttttgcaaag gatgtgaaca ataccggaat atttgaaagt gtctgtgtta
     5281 tattggaatt accgacaccg gacattatgg tttcggcaag ggaaagagct gtagcatgag
     5341 cgaaaaatat cgcggctgaa agagcacctg ctagcatgaa aagagttaaa acattaatta
     5401 tcagatttat attatgtttg cgtaaaaaat atataaattc atgcagatag cctagcacag
     5461 ttcttttttt cgcagcataa attgatggca aaaaccagaa accgattgta agtgttaaaa
     5521 ccgttaaaag agcaccgata tatatcggta agaaagttaa agcaaaaatt agagtgcctc
     5581 catccgggat tcgagtaatg aataccagta atgtttgtat agctattagg gctggagcac
     5641 ctccgaaaag tgatgcaaga agggaatttg caaataattt tagtcccgct gtgttgttga
     5701 aaagctgaaa tccattagta ataagtagtc tttttgcgga aacggaggca aaagcggtat
     5761 atacagcgaa aaccagtaga ggcggaatta tatttaaaaa tgacaaaaga taattaccgg
     5821 atgcttgagg tgggggtgtt aaaaaagtaa ttatatttgt acttataaag aatatagaaa
     5881 tcattgaaat tatggatata aatgatagtg aaaaatttcg aagagtaaat gcttttgaga
     5941 tgagtttaaa aacttctgat aaagaagttt cttgcaaatt ttgttgtcgg ggaggctcta
     6001 ttttctttat aacttcgctt gaattaacag attcggtttt tttagtttcg ggttttgatg
     6061 ttgaaacagg tttttgtaaa actggtgctt tctctatcgg ttctgtgact cgcgtaataa
     6121 aaggcatttt gtgtttttgc tcaggatcct cgggaatatc aatgtagaat ccatatccgc
     6181 attgtttgca ttttgttgca tatctgttag gttttagatt tgctgtcgaa atagagtagg
     6241 atatgttgca ttcaggacaa aaagttattt tgatcggatt tgagcaagaa gggcaatgag
     6301 tcataccatt ttgaaaacta ttaattttct cagatgagag gttaaatgta tgtccgcatg
     6361 actggcattg agttgaaata attttgtgtt tcacaacaga ctttgcagaa gtttgcatga
     6421 tttatctcca gtcgctataa ttaaaataga tcatatatac ttttcatcga ttcatgtttg
     6481 cttgaacagc gtttttttgg tgctgttccc tcgagaaaat actcttgaat aacaggacaa
     6541 ccctccgaag gcagatcgcc tgaatgctcg caaactgata tttgaataat ttcgggaggt
     6601 tttggtggaa aagcaagttt acgacgtatt gaggctgttt ccttcatgaa atttgcccac
     6661 acaggagctg ctacatttgc gccatattgc cccggcccaa gcgtaaattt ttgcgaatca
     6721 catccgatcc agacagcggc tgcaagatct ggagtgtaac ccgcaaacca tgcatctcga
     6781 taatcactgt tagtacctgt tttgccagcg gcgggggatg tgaaaccgca tctgttacga
     6841 attgtggagg ttgcagttcc gtttgtaata accgcttgta atatggagtt cataataaag
     6901 gctgttccgc gtgaaaaaac cgccttttgt gtttttggct cgtgaacata gatttcttta
     6961 ttgtgattat caaggatttt ttgtattgca taaggcttta ccggtattcc ttgattcgcg
     7021 atacatgcaa atcctgttgt tagttcgagt ggtgtaagct ctgatgagcc taatccaagg
     7081 gttggatcaa tagtgaatcg gtttttaccg atatgtatcg agcgagacgc ataagaggcg
     7141 attcgatccc ccccaatttt ttcgtatgcc aataccgcaa tagtatttat tgaccgtgta
     7201 attgcttttc tgactaggac aggtcctgaa aacttatcag aagcattaga aggagcccat
     7261 gttttcgttc tttcattaaa aaccataggc aaatcctgga attgagtagc tgctgtaatg
     7321 attttgtctt ctattcctga tgcgtaaaca aacggtttaa acgcagaacc gggctggcgt
     7381 cgtgaattaa ccccgcggtt aagttgattc tcatagctga atttctttcc acccacaaga
     7441 accttgattt ttcctgaatc aggttcaatt gcagtgaaag cgccttcaac gttagcgagt
     7501 tgacgttgtt gtcggtagaa tctgctgtaa ttagagaaaa tatcagtaat tttatcagca
     7561 ccacatataa gagctaaaaa tgatgtttca tcggaaattt gactgcgtat gtccttaagt
     7621 gctgttattt catttattga tattttttgc tcttttgaga tataaaaatc attttcgaga
     7681 attgctagtt ctctatcgtt atattttgat gaaattttct gttgttgtgt tattctttct
     7741 gtaagtagtt cttgcgcgat ttgctgcatt tttaaatcaa aagttgtgta aacctttagt
     7801 ccacctttgt agactatatc ttctccatat ttttctataa gctgctggcg aatatagtcg
     7861 gttagatatg gcgccttgtc atcatttttg tttcgagcta tttcgtttgg ggatctgtcg
     7921 atgatttgct caagatatcg tgaccagaat ttttcaaaat tataaagtgc atcttttttt
     7981 gatatatgac cattttggac aagattaaca ataatttgtt tgtgtttact gaatgttatg
     8041 cgcgggttgc gaagtggtga atatctgttc ggagcagggg ggataagagc aagtagagaa
     8101 gcttcgtata tatctatatt ttttaattct tttccaaaga aaaagtctgc tgcagatttg
     8161 attccataaa caccatgccc gaaatatatc tgatttaaat acatttcaag tatttgattt
     8221 tttgtgaaat tttcttctat tttcttgata agcagaattt caataatttt ccgttcaatt
     8281 gttctttttt tatgagtata aagttgcttg atgagctgtt gagttattgt tgacccaccc
     8341 tgttttatag atccctgttt aatgtcgaca attaaagctc tcattattcc tacaaaatcc
     8401 agacctctat gtttaaaaaa atttttatct tctgctgaag taaatgctgt gataacggta
     8461 tcgggtattt gctcgaattt aacaggagat cttttttcgt cgtatagttc tgcaattatt
     8521 gtaccattag aatcaaataa cgtagttggg agattcgttg tatattccaa atcacttgaa
     8581 ataagatcgt attgagaatg atacatgtaa acaaaaagaa agtagggagt tagtagcaat
     8641 gtaactaata ataatatgat tgaaaacctt ttatgcattt tggctgtatc cctaaaaatg
     8701 agaaatagtt gtattatagt atcggtactt atggatatta agtaaatagt caattctcta
     8761 tgatttttag atattaaatt ttacttgata ttaattgtta aggactattg gctgattaag
     8821 tattgatttg tttattagat cactttgatt gttttataca gtggttaact atagtgaagg
     8881 atgcttttat gaaaatgtct gtaagaggtg agttggtatt agtttggttt tttcttggtt
     8941 atgggttata tttctttacc ggaatttata cttttttacc aatgatgatt tttttcgcac
     9001 ctatatttat attgcatctt cttgaaaaac aagaactttt tgaagcatgg attttctctt
     9061 tcatcggatt tgtgttggct gtgcagttgt caactctacc gttttccgat tatgaaggtg
     9121 taaactggtt gtattcgggg gtattaaaga attttataat tgctgcgggg attgctttgc
     9181 cgtattggtt gacccgactt gtttatcata aaaatagtgg ctttgctcat actttttcct
     9241 tcccgatttt tactacttct gtatatttta ttttatccaa agtaaacttt tttaatgggc
     9301 tttcgggttt ttctcattat ttacaggccg ataatgagtg gttgttgcag tttgtgtcgt
     9361 tatccggtat atggattaca gtatttttta ttagttggac tgcgtcgatt gcatgttgga
     9421 tatggaataa tagtgttaac ttaggacgcg ttggcgg
//
LOCUS       DAOTGZ010000097         9434 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_3064
            NODE_8668_length_9434_cov_4.031453, whole genome shotgun sequence.
ACCESSION   DAOTGZ010000097 DAOTGZ010000000
VERSION     DAOTGZ010000097.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868088
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 9434)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9434)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:22:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,785
            CDSs (total)                      :: 2,751
            Genes (coding)                    :: 2,732
            CDSs (with protein)               :: 2,732
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 12 of 19
            Pseudo Genes (internal stop)      :: 5 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9434
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8668_length_9434_cov_4.031453"
                     /isolate="AMR_MDS_3064"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            197..997
                     /locus_tag="P5123_07610"
     CDS             197..997
                     /locus_tag="P5123_07610"
                     /inference="COORDINATES: protein motif:HMM:NF018184.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HRX16164.1"
                     /translation="MYTIGQFAAVTKLPARTLRFYDSIDLLKPALVDEDNGYRYYNEK
                     SLLIARQILIFRECGMSLKSIGKIIDKNESSEDIVTVLKGHLNTLDDRLKELEYSRSK
                     FEKIFSAYQNSEAEEIVVQKRDSLKVISVRERGDHSTISSMLSRIFETAAVKHIEITG
                     PHTIVYHEPKDLEAEDHDMEIYVPVEENSEDCGLIKVIPSKQFCKLIHRGSMSMLAES
                     YNKVLSYIDKKDLKITGPFEEIYTVGGKFFKPSSLKIEIAIPVGEDHE"
     gene            complement(1161..1598)
                     /locus_tag="P5123_07615"
     CDS             complement(1161..1598)
                     /locus_tag="P5123_07615"
                     /inference="COORDINATES: protein motif:HMM:NF012780.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HRX16165.1"
                     /translation="MTANDLLKRQTGGLFSVSANTSVHECAALMSEKHVGALVVLDAK
                     GNLVGIVSERDIIRILSKKQISDSTTVKEIMVPSEKIISSQKTDSITDVMEKMNENYI
                     RHIVIKDGDRLLGLGSIRDVVRMVLDSTLLENQQLKDYVYQAK"
     gene            2161..2385
                     /locus_tag="P5123_07620"
     CDS             2161..2385
                     /locus_tag="P5123_07620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003867363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase TusA family protein"
                     /protein_id="HRX16166.1"
                     /translation="MAEKTLDCLGEACPVPLVKAQKELEKMQKGDVLIVNIDHSCAMK
                     NIPDWAREAGHNVEIDEVGEGEWDIVIEKC"
     gene            2434..2976
                     /locus_tag="P5123_07625"
     CDS             2434..2976
                     /locus_tag="P5123_07625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007870708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YeeE/YedE thiosulfate transporter family
                     protein"
                     /protein_id="HRX16167.1"
                     /translation="MSEEKINANKQKVDYWKILFKNEWSYVTGAVVIVVLATALVIVT
                     GKSWGVTGILHLWGGKFLELIGVNADTWSIYNGALGKFNFWKSIPSITNVGIVLGALL
                     SALLAASFKIKKIKSYKQVIAAILGGILMGVGARMAMGCNIGALFSGLSSFSLHGWVF
                     FLFIFIGAMVESSMLKKWFM"
     gene            2999..3709
                     /locus_tag="P5123_07630"
     CDS             2999..3709
                     /locus_tag="P5123_07630"
                     /inference="COORDINATES: protein motif:HMM:NF016064.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YeeE/YedE thiosulfate transporter family
                     protein"
                     /protein_id="HRX16168.1"
                     /translation="MSENIGRIARSSGRRTQMSDAQRKRRKAVQIITGAALIAVIIVM
                     AFVANQTNPKGAYIVIIGTLLGYALQRSRFCFTAALRDPFLTGGTNLTKAMIITFALY
                     SFLLMAINITKFGISLDSVDFSKAAGYIRPVGFHTALGAFLFGIGAVIAGGCASGTFM
                     RMGEGFLQQWVVVIFFIIGSMIGGVAAPIVQFKSAPVVFLPQLFGGWALAAFVQFSLL
                     LILYIVADIWGRKKAGGR"
     gene            3713..4942
                     /locus_tag="P5123_07635"
     CDS             3713..4942
                     /locus_tag="P5123_07635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006675854.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HRX16169.1"
                     /translation="MEKKKEYYDVVVIGGGPAGMTAALYSGRARLKTLLIEKSLIGGM
                     ATYTSEIENYPGFVDPIDGIELMKLFDKQFRRFGVDVKLTDVKSVAVSCSDRIVTTFR
                     TDYYAKAVIIATGNKPRLTGAQNEDQFLNDKGISFCATCDAARNTDKKVMIIGSGDAA
                     IEEGMFLTKFASQVTVSVMHDEGIMDANKVAQEKALSNNKMKFVWNTVVNSFEGDDSG
                     LHRVILKNIKTGDLIPVETDACFLFIGYIPNTSVFKGLINLNERGYIQTNESMETNIP
                     GIYAVGDCRDKILRQVATAVGDGAIAGFMVEKFIEEENYIQKEIFDAEGLIMAYIYNA
                     SEALDREYLVRVEEIQKKYSDKIKLRRIDIYKSDRIACRLQFTKSPSLIIMEKGEILK
                     NITDLSDIDAAIAAIVK"
     gene            5168..5824
                     /locus_tag="P5123_07640"
     CDS             5168..5824
                     /locus_tag="P5123_07640"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRX16170.1"
                     /translation="MLPIVIVSIFAFLAFVFMLFFTALMIFSKKKDNRSMMPYLAGLL
                     VSLFVCGLLVVLDIYLAVQFAYSNKEKIVEVADESVDKVLKVSADLTSRSIRYTTADI
                     WNNWDKDYLEKLENVDLEIKNVKAEKKKGSKVYIIDAIISNKNEDSVTLSLKKAISNN
                     YLFGLDKDEICYSVALVDKSFSSDYIPIGRSRVKLQVVNDEKEDIVDLQFGKKTYSLK
                     "
     gene            5941..6108
                     /locus_tag="P5123_07645"
     CDS             5941..6108
                     /locus_tag="P5123_07645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007934692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HRX16171.1"
                     /translation="MHVISSECITCGACKSECPVDAIHEGDDQYTIDENCIDCGACVA
                     VCPVDAISMKE"
     gene            complement(6153..6225)
                     /locus_tag="P5123_07650"
     tRNA            complement(6153..6225)
                     /locus_tag="P5123_07650"
                     /product="tRNA-Trp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(6190..6192),aa:Trp,seq:cca)
     gene            complement(6290..6544)
                     /locus_tag="P5123_07655"
     CDS             complement(6290..6544)
                     /locus_tag="P5123_07655"
                     /inference="COORDINATES: protein motif:HMM:NF013844.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Smr/MutS family protein"
                     /protein_id="HRX16172.1"
                     /translation="MSIIIPEIDLHLTPKDCTHDILTDFIETAVNNGADTIRIIHGKG
                     NSSKKLEVYKILEQNRSVYGYDDDGSNWGATVVHLEAKEK"
     gene            6763..7542
                     /locus_tag="P5123_07660"
     CDS             6763..7542
                     /locus_tag="P5123_07660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002723903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="motility protein A"
                     /protein_id="HRX16173.1"
                     /translation="MDIATIGGLIAGMVCLLIGIIAAKGDLGLFWDEASILITIGGSL
                     SALLASNPLQRFLNIGRAIKVAFFAPNNSPNDMILTLISFSEKARREGLLALEDDLDE
                     LPDEFLKKGIQLVVDGTDPEMVRRIMETEIEQMIDRHDAMKKIFDDWASLAPGFGMIG
                     TLIGLVLMLVNIEDKSAIGPGMSAALITTLYGAIIANWLSIPVANKMEARANEEVLMK
                     LVMIEGTISIQAGDNPRLVKDKLIAYLEPAEREAISNEVEG"
     gene            7542..8318
                     /locus_tag="P5123_07665"
     CDS             7542..8318
                     /locus_tag="P5123_07665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014624588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HRX16174.1"
                     /translation="MKKKKKCPPPAPSWLTSWSDLTTLLLTFFILMFSTADIDGKDFF
                     LILSSFRGSLGMFEGGHTLSKGRLEEMGLNINTLPSDAKKRSLAQQLKKALEAFKPEI
                     QSKKVRISEDERGLIITLASDAYFDPGSAALKEEIRPVLRKVTSIMGAIPNFVRIEGH
                     TDNNPISPGTTEARYETNWELSSARSVNIIRYLTEVEEAEPKRLSAVGFAQYRPVDDN
                     NTPEGRAFNRRVDIVILRDQIYQESDKPGIERPLPGEEWR"
     gene            8482..9006
                     /locus_tag="P5123_07670"
     CDS             8482..9006
                     /locus_tag="P5123_07670"
                     /inference="COORDINATES: protein motif:HMM:NF015693.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body-associated FliL family
                     protein"
                     /protein_id="HRX16175.1"
                     /translation="MGDEDLNDDIDSGEESVEEAGSAGASKLIKILMFVVGAIAGIFV
                     IILISYTVSKYVQERAYQREQDIVVAPPPPPLSHFSLPDFAVTTKDAEPHFAKISISL
                     GYEDNVELNTELIRRTVQIQHLVNIILRGKRYEDLDSVEDTVALAEEIKAHVNVILIS
                     GKIKEVYFREFVVN"
     gene            9076..>9434
                     /locus_tag="P5123_07675"
     CDS             9076..>9434
                     /locus_tag="P5123_07675"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRX16176.1"
                     /translation="MGDGSLSQDEIDALLKGTDDVAPQQSDQSSVSSGAGSAGGGSLS
                     AAQKGALTDMMNSVMAAVAPGLSGYLQNDLSIGNAYIESKDGSSLSGDFSGQYVQIQM
                     GFNGGVTGQNSVLMSAT"
BASE COUNT         2807 a         1505 c         2115 g         3007 t
ORIGIN      
        1 caagcgcata gcagatcttc atggttttga tataacggtg ctgagtagtg aagagtttac
       61 cgatttcgta gtgagttccg gtaaaaatat tttactgtta acataaagga attttacgcg
      121 ggtacactgt aaaaaatctt tgaccttcca cttactgaaa gccttatttt ttagcaaacg
      181 taaaaggagg catatcatgt acacaatagg acagtttgcg gccgttacaa aactaccagc
      241 acgtacactc aggttttacg attccatcga tcttttaaaa ccggcactgg ttgatgaaga
      301 caacggttat cggtattata atgagaaatc tcttttaatc gcacggcaga tactgatatt
      361 cagggagtgc ggtatgtctc taaaatcgat tggtaaaata attgataaga atgaaagcag
      421 tgaggacatt gtaacagttc ttaaagggca tttgaacact cttgacgata ggctcaaaga
      481 gctcgagtac agccgctcaa aatttgaaaa aattttttct gcttaccaaa atagtgaagc
      541 tgaagaaata gtcgttcaaa aacgtgattc gttgaaggtt atttctgttc gagagagggg
      601 agatcattca actatcagtt ctatgctaag ccgaatcttt gaaacagctg ctgttaagca
      661 tatagaaata accggtccgc ataccattgt gtatcatgaa ccaaaggatc ttgaagcgga
      721 agatcatgat atggaaattt atgttccggt cgaagagaac tctgaagatt gtggtctgat
      781 aaaagtaatc ccatcaaagc aattttgtaa actaatccat cgcggcagta tgagtatgtt
      841 agccgaatca tataataaag ttctaagtta cattgacaaa aaggatttaa aaattacagg
      901 cccgtttgaa gaaatctata cagtaggtgg taagttcttt aaaccatcat cgttaaaaat
      961 tgaaatagcc atacctgttg gggaagatca tgagtaaaaa taaaaaagat taactaactt
     1021 ttattttagc gataaaagtc ttaaagataa tttatccgaa tacttgaaat aaaaatccag
     1081 gcacaacttt ttagatagtg cctgaaataa ataatataga gatttacggc atgtaataag
     1141 cacaatatct cgataggctt ttatttcgcc tgatagacat agtccttgag ttgttgattt
     1201 tcaagaagtg tactgtctaa taccattctt acaacgtcac gtattgatcc aagccctaac
     1261 aacctgtctc catccttaat aacaatatgt cttatgtagt tttcattcat tttttccata
     1321 acatcagtga tagagtctgt tttctgagaa ctaataattt tttcagacgg aaccatgatt
     1381 tctttaactg tagttgaatc ggaaatctgt ttttttgaaa gaatacgaat gatatcacgt
     1441 tctgacacga tgccgactag attacctttt gcatccagaa caacaagagc tccgacatgt
     1501 ttttcagaca ttaaagcagc gcattcatgt acagatgtgt tagcggatac agagaataat
     1561 cccccagtct gtctttttaa aagatcgtta gcggtcatgg tttttctcct tgtttcgtgg
     1621 ctgaagcgat gactacaagg gcaggtgtaa tccacatatt tttagatcac ctataaaacc
     1681 tgtttgtaga taaacttgat cagcaaacta caaacagctt gggttttcgt agaagtggga
     1741 ggataacaag ttttacataa caattattta ttatagtata gttatatcta ttttgtcaag
     1801 aaattgtgca aatcttttga acattttaaa aaaaatgata tttttagatg aaaattatat
     1861 ttagtttgtt attataagtt ttgcgactta ataatttgtg aatattctta atatgagtat
     1921 aacataaaaa gggttaagtt atagctcgtc tgtaagtgcc tgcaaattga gaaccccgcc
     1981 atatttatat gtttatgatc tttattactg ttatgggaaa aaattttcag aactttggtt
     2041 ttgtgtgtgt tcttgtgctt tgttaccggt atgaatgtaa ggtgtctgat cggaacaaaa
     2101 aaaatcatgt tttgtttcgt gatgagtagc ccataaattt ttaaacaaag gagaattgtt
     2161 atggcagaaa aaacattgga ttgtcttggg gaagcttgtc cagtacctct tgttaaggct
     2221 caaaaggagc ttgagaagat gcaaaaaggt gatgttttga ttgtaaacat tgaccacagt
     2281 tgtgcaatga aaaatatccc tgattgggca cgggaagcgg gtcacaacgt agagatcgat
     2341 gaagttggtg aaggtgagtg ggatatagta atcgaaaaat gctgaacatg tggttccact
     2401 ccttaaatta ttattaatta aggagaatgg tttatgtctg aagaaaaaat taatgctaac
     2461 aaacagaaag ttgattattg gaaaattctt tttaagaatg aatggtcgta tgtaacaggg
     2521 gctgttgtaa ttgttgtgct ggcaactgct cttgtaatcg ttactggaaa gtcctggggc
     2581 gtaacaggca tcttgcacct ctggggtgga aagtttctgg aattgatagg tgttaacgct
     2641 gatacatggt ctatctataa tggagctttg ggaaaattta atttttggaa aagtattcca
     2701 agtataacta acgttggtat agttctcggt gcgttgcttt cagcgctttt ggctgcttcg
     2761 tttaaaatca aaaaaataaa atcatataaa caggttattg ctgcaatact gggtggaatt
     2821 ttaatgggag tcggagctcg tatggccatg ggatgtaata ttggggctct tttttcaggg
     2881 ttatcgtctt tttcactgca tggctgggtc ttctttctat ttattttcat tggagccatg
     2941 gtcgaaagca gcatgctaaa aaaatggttc atgtaacaaa taataaggag gtcattatat
     3001 gagtgaaaat ataggtcgaa tcgcacgatc atcaggtcga agaacccaga tgagtgatgc
     3061 acaacgaaaa agacgaaaag ctgtccagat aattaccggg gcagctctta tagcagttat
     3121 aatagttatg gctttcgttg ccaatcagac aaatcctaaa ggtgcatata tagtgataat
     3181 aggtactcta ttaggttacg ctttgcagcg cagcagattt tgctttacag ctgcgttgcg
     3241 cgatcccttt ttaactggtg gaactaatct gacaaaggct atgattatta cttttgctct
     3301 ttattcattt cttttaatgg caattaatat aacaaaattt ggaatatctt tagactctgt
     3361 agatttttcc aaagctgctg gatatatcag accagtcgga tttcatactg ctcttggagc
     3421 atttctgttt ggtattggcg ctgttattgc tggcggttgc gcttcgggaa catttatgcg
     3481 tatgggggaa ggttttcttc agcaatgggt ggtcgtaatc tttttcatta ttggttcaat
     3541 gattggtggt gtcgcggcac ctatcgttca gtttaaaagc gcgccggttg tttttttacc
     3601 acaactgttt ggtggatggg cacttgccgc ctttgtccag ttttctcttc tgctaattct
     3661 ctatatagtt gctgatatat ggggacgtaa aaaagctgga ggcaggtagc ttgtggagaa
     3721 aaagaaagag tattatgatg tagttgttat cggtggagga cctgccggca tgaccgcagc
     3781 tctgtacagt ggaagagctc ggcttaaaac attgttaatt gaaaaatctt taatcggtgg
     3841 aatggccact tatacaagcg agattgagaa ctatcctggt tttgttgatc caatcgatgg
     3901 tattgagtta atgaaactct tcgacaaaca atttagacgt tttggagttg atgtgaaact
     3961 taccgatgtt aagagtgtcg ctgtttcctg tagtgatcgt atagtaacta catttcgaac
     4021 agattattat gctaaagccg taattatcgc aacaggtaat aaaccccgac ttaccggtgc
     4081 tcaaaacgaa gatcagtttt tgaatgataa gggaatatct ttttgtgcga cctgtgatgc
     4141 agcgcgtaac actgataaaa aggttatgat aatcggttct ggggatgccg cgatcgaaga
     4201 ggggatgttt ttaacaaagt ttgccagcca ggtgaccgtt tctgttatgc acgatgaagg
     4261 tataatggat gccaacaagg ttgctcagga aaaggctttg tcaaataaca agatgaagtt
     4321 cgtctggaat actgtggtaa attcttttga gggtgatgat agtggtttgc accgtgtgat
     4381 tttaaagaac ataaaaacag gtgatttgat tcctgtcgag acagatgcct gttttttatt
     4441 catcggttac atcccgaaca cttcggtttt taaggggttg atcaatttga atgagcgcgg
     4501 ctatatccag accaatgaat ccatggagac aaatattcca ggtatttacg ctgttggaga
     4561 ttgccgtgac aagattcttc gtcaggtggc aaccgctgtt ggtgacggcg cgattgcagg
     4621 ttttatggta gagaagttta ttgaagaaga aaactatatt cagaaagaaa tttttgatgc
     4681 tgaaggactg attatggcct atatctataa tgcatctgag gctcttgatc gtgaatatct
     4741 tgtaagggtt gaagagattc aaaaaaagta tagtgacaaa attaaacttc gacgaattga
     4801 tatctataaa tctgatagaa ttgcctgtcg tcttcagttt acaaaatcgc catccttgat
     4861 cattatggaa aaaggtgaaa tattgaagaa tattactgat ttgtcagata ttgatgcggc
     4921 tatagcggca attgtaaaat aattcgttga tttggattct gtgttttgta ataccctctt
     4981 gatgaatgtc gagagggtat ttatttggaa ttgtaagaat ggtggagtgg ttgtaaccat
     5041 aaatatcaga tacaatgcga tttgttttaa tatatcaaaa gattattgac aattgtgagt
     5101 tattgtaatt taaaatatct tgttttaatt ttattatcgt tatatttaca gtattgagga
     5161 gagtcttatg ttgcctattg tgattgtatc catatttgcc ttcctagctt tcgtattcat
     5221 gttgtttttt acagctttga tgatatttag caaaaaaaaa gacaacagat caatgatgcc
     5281 atatcttgcc gggcttcttg tttcactgtt tgtttgtggt ttgcttgttg tgcttgatat
     5341 atatctggca gtgcaatttg catatagtaa caaggagaaa atagtcgaag tcgcggatga
     5401 gtctgtagac aaggttctga aggtttcagc cgatttgaca tcgcgttcca tcagatacac
     5461 aacagctgat atttggaaca actgggacaa ggattatctg gaaaaactgg agaatgtgga
     5521 tcttgaaata aaaaacgtca aagcagaaaa gaaaaagggt tcaaaagtat acataattga
     5581 tgcaataata agtaataaaa acgaggattc cgtcacgctt agtttaaaaa aggcaataag
     5641 taacaattat ctgtttggtc tagataaaga tgagatttgt tactctgtgg ctcttgtaga
     5701 taaaagcttc tcatcagatt atattcctat tggtcggtct agagtaaaat tacaggtagt
     5761 aaatgatgaa aaagaggata ttgttgatct gcaatttggg aaaaaaacat attctttaaa
     5821 ataaatatat ggaaatttat ttaataatgc tatttactaa aagatatctg ttttttcttt
     5881 gttagcattt atctttttgg aagattttgt aaaatatatt tttaattacg ggaggatttt
     5941 atgcatgtaa taagtagtga gtgtataact tgcggtgctt gtaagagtga gtgcccggtt
     6001 gacgctattc acgaaggtga tgatcaatat acaattgatg agaattgcat tgattgcgga
     6061 gcttgcgtgg ctgtttgtcc tgttgatgct atctcgatga aagagtagtt attgtttaaa
     6121 aaaagcaacg gtatataccg ttgcttttaa tgcaggcgca gagggactcg aacccccaac
     6181 cgacggtttt ggagaccgca accctaccaa ttgggccatg cgcctattga taagattgac
     6241 tatgaaaaag cagggctttt ctgtcaagaa aaatgccaaa ttgtcacttt tacttttctt
     6301 tagcttcaag atgcacaact gtagcacccc aattagagcc atcatcatcg taaccgtata
     6361 cagatctgtt ttgctccagt attttatata cttccagttt ttttgatgag ttgcctttcc
     6421 cgtgaataat tctgatcgta tcagctccgt tattaaccgc tgtttcaata aaatctgtca
     6481 aaatatcatg tgtacagtct ttaggggtta aatgaaggtc aatttctgga ataattatgc
     6541 tcatctttat aactccaaat aattagctaa tatatggaaa aatttcagta tatgcgggca
     6601 taatcgttca ataatcataa tattagacta ctttttttta caaatcatgt cgaaaattta
     6661 agtaatggac accaattacg ggttatttgc aaggaatcta ttgctgttca tgtctttcgg
     6721 acttctgttc cgctacagct gttatgataa acggagtttg ttatggatat agctactata
     6781 ggcggtttga tcgccggtat ggtctgcctg ttgattggta ttattgctgc caagggtgat
     6841 ctggggcttt tttgggatga ggcatcgatt cttattacaa tcggtggttc gttatcggct
     6901 cttttggcat caaatccgct tcagcgcttt ttaaatatcg gaagggcaat caaggtcgct
     6961 ttttttgcgc ctaacaatag cccaaatgat atgattttga cgcttatatc attttctgaa
     7021 aaagcaaggc gtgagggact tcttgcgctc gaggatgacc tggatgaatt gccagacgaa
     7081 tttttgaaaa aaggtattca gttggttgtc gatggaactg acccggaaat ggttaggcgt
     7141 attatggaga ccgaaatcga acagatgatt gatcgtcacg acgcaatgaa aaagatattt
     7201 gatgactggg cttcgttagc tccaggtttt ggtatgattg gtacactgat tggacttgtt
     7261 cttatgcttg taaacataga agacaagtct gccatcggtc ccggtatgtc tgccgctctt
     7321 attacaaccc tgtatggtgc gataattgct aactggcttt ctatacctgt tgctaacaag
     7381 atggaggctc gtgccaacga agaggtgtta atgaaactgg tgatgataga gggaacaatt
     7441 tccatacagg ctggtgataa cccgcgtctg gtaaaagaca agcttatcgc ctatcttgag
     7501 ccagccgaaa gagaagccat atccaatgag gttgagggtt aatgaagaaa aaaaagaaat
     7561 gtccaccacc ggcaccgtca tggctgacgt cctggtcgga tttgacaaca cttcttctaa
     7621 ctttctttat attgatgttt tcgactgcgg atattgatgg aaaagatttc tttcttatct
     7681 tatcatcttt tcgcggttca cttggcatgt ttgaaggtgg tcatacgctt tcaaagggca
     7741 ggttggaaga gatggggcta aatattaaca ctcttccgtc tgatgctaaa aagcgttctc
     7801 ttgctcaaca gttaaaaaag gctctcgaag catttaagcc cgagattcag tctaaaaaag
     7861 tgcgtattag cgaagatgag agagggttaa taattacatt agcgagtgac gcctattttg
     7921 atccggggtc ggcggcgtta aaagaagaga taaggcctgt tttgcgtaag gtcacttcaa
     7981 ttatgggggc aattcccaat tttgtgcgta ttgaaggaca tacggataac aatccgatca
     8041 gtcctggcac aaccgaggct cgctatgaaa caaactggga gctttcgtcg gcaagaagtg
     8101 ttaacattat ccgttatttg acagaagtag aggaagcaga gcctaaaagg ctttcggctg
     8161 ttggttttgc tcagtatcga cctgttgatg acaataatac tcctgaaggt agagcattta
     8221 accgaagggt ggatatagta attttaagag accagatata tcaggaatca gataagcctg
     8281 gtattgaaag acctcttccg ggtgaagagt ggcgttaata atcaagtacc tgaatatgtt
     8341 aaattaattg acaggagaat tatctgtatt attatgtcgc atgaaagtgt acccggatat
     8401 caatctggct tcggtcgata aagcgaaaat aatatgttat acaacattat ttcggtgttg
     8461 tatgtctgtt gagaggattt tatgggtgat gaagatctga acgatgatat agactccggt
     8521 gaagagtccg tcgaggaagc gggttccgcg ggtgcctcca agcttatcaa aatcctgatg
     8581 tttgttgttg gggcaatcgc tggtatcttt gtaataattt taatttcgta tacagtatca
     8641 aagtatgttc aagagagggc gtatcagcga gaacaagata tagttgttgc tccgcctcct
     8701 cctccgttgt ctcattttag tctgcctgat tttgcggtaa cgaccaagga tgctgaacct
     8761 catttcgcca agatttctat atccttgggc tacgaagata atgtagagct caataccgag
     8821 ctgataaggc gtactgttca gatacagcac cttgtgaaca ttattctgag aggcaaaagg
     8881 tacgaggatc ttgattctgt ggaagatacg gtggcgcttg ccgaagagat aaaggcgcat
     8941 gtgaatgtga ttttgatttc aggtaaaata aaagaagttt attttagaga atttgtagta
     9001 aactgatgtt ttatgttaat gaggtggctc ttcccttaga gagttaccat acgatactca
     9061 agtgagaggt ttgtcatggg tgatggttcc ctttctcaag acgaaataga tgctctgtta
     9121 aaaggtactg atgatgttgc accacagcaa tcggatcagt cttctgtgtc ctctggtgcc
     9181 ggcagtgctg gaggtgggtc gctttcggcc gctcagaaag gggcattgac cgacatgatg
     9241 aactctgtta tggccgcggt agctccgggt ttaagcggat atctgcaaaa tgatttgtct
     9301 ataggtaatg cttatattga gtcaaaagat ggatcttcct tgtcgggtga tttttctgga
     9361 cagtatgtac agatacagat ggggttcaat ggtggtgtaa cagggcagaa tagtgtgtta
     9421 atgtcagcaa ccga
//
LOCUS       DAOTGZ010000098         9320 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_3064
            NODE_8817_length_9320_cov_4.689153, whole genome shotgun sequence.
ACCESSION   DAOTGZ010000098 DAOTGZ010000000
VERSION     DAOTGZ010000098.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868088
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 9320)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9320)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:22:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,785
            CDSs (total)                      :: 2,751
            Genes (coding)                    :: 2,732
            CDSs (with protein)               :: 2,732
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 12 of 19
            Pseudo Genes (internal stop)      :: 5 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9320
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8817_length_9320_cov_4.689153"
                     /isolate="AMR_MDS_3064"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..270
                     /locus_tag="P5123_07680"
     CDS             <1..270
                     /locus_tag="P5123_07680"
                     /inference="COORDINATES: protein motif:HMM:NF013073.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NlpC/P60 family protein"
                     /protein_id="HRX16177.1"
                     /translation="DPVTGSTVNHVGIYLGKDKFVHMPGEGAYVRIERLVKDSRWSAR
                     FAGVRSYHKLFDDFTEKSSKSPDKKEKVQAFKNMDPDVQFETSDM"
     gene            complement(274..2100)
                     /locus_tag="P5123_07685"
     CDS             complement(274..2100)
                     /locus_tag="P5123_07685"
                     /inference="COORDINATES: protein motif:HMM:NF018884.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3369 domain-containing protein"
                     /protein_id="HRX16178.1"
                     /translation="MTCLLSYTCLLKYQLWAIRGVPLKTRQNADDLVFFDDSQEEIYE
                     KKLRETWKILIVDDEQDVHKVTKLVLSDLEFSGRSVEFLSAYSDAEARQILNERDDIA
                     VILLDVVMEQENSGLLLTQYIREELQNSLIRIILRTGQPGQAPEKRVIVEYDINDYKS
                     KTELTTERLFSTIITALRSYRDIITIEKSKRALEKIIDATAHIFEVQSLKNFVSGILD
                     QFTSILRYSNNAFYCQTSGLTAINVDGHPVILAATGKYQNSRPNTRAKDVLPAEEYQL
                     LEIAFNEKRNVFDDYHYIAYFKTQNNSENIIYLERDEALQEWERDMIDIFCSNVSIAF
                     ENIYLNQELENLVENRTQQLKHANYELNIKNSTFERELQMARSVQQIIIPKKFPESQF
                     FQIGGRYHPMQNLGGDYYDVFPFTENSMGIAIVDVSGHGISAALITTMAKMSFASLAK
                     PHNPPFYVLDEINSSLLKTVGGAGMFLTAFYGIIDLSINELIYSNGGHNEVLLMHKDG
                     SFAELGSLNPIVGMFPEEEYVSSSCPIHSGDILVLYTDGIVESKNSEDEFLGKSRLME
                     ILRQNSELNPDELSELVLQKVYQFTGSKEPEDDLSILITRII"
     gene            2377..3045
                     /locus_tag="P5123_07690"
     CDS             2377..3045
                     /locus_tag="P5123_07690"
                     /inference="COORDINATES: protein motif:HMM:NF024895.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane beta-barrel protein"
                     /protein_id="HRX16179.1"
                     /translation="MKKIIYFIAMMSVFSGFSVNALAGNVTELSMGATQADAVGDDWG
                     LNVSAAWWNELNYMFALGFNPQFVWFQWDKYVTDNNGDKIYQEKEVGGVVDEYPIVEQ
                     ANAYLIPLHIVAKVQFPLSDTVTPYLKGGGGYTFMPLTYSDADSEMFGGATWTAAVGC
                     QFRVPDIPNFRFFAEFGYRGSKLTNDDNVQIDMSGVVIHAGVQYGLTRDKPRSATTDS
                     GIVW"
     gene            complement(3059..3580)
                     /locus_tag="P5123_07695"
     CDS             complement(3059..3580)
                     /locus_tag="P5123_07695"
                     /inference="COORDINATES: protein motif:HMM:NF025743.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4416 family protein"
                     /protein_id="HRX16180.1"
                     /translation="MAKAIIPTKALLFAGVLTSSDQLVLSTLKEMGARYGKIDTITQK
                     IPFDLSSYYNSIGENLYKIFFSFTELVDRNMIAEIKIFTNNLEEKLSSSHETRKINID
                     PGYLTLSNVFLASCKEYYHRMYVDRGVYLENEYYFTKGKFEFFDWTYPDYKKDDYLDY
                     FMMLRKKYRLMFS"
     gene            complement(3582..4358)
                     /locus_tag="P5123_07700"
     CDS             complement(3582..4358)
                     /locus_tag="P5123_07700"
                     /inference="COORDINATES: protein motif:HMM:TIGR00056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HRX16181.1"
                     /translation="MILSFIRKSNQFFEGFGNYAIFFKETVRWIFKPPFDFKNLIQNL
                     LEIGYKSIPVTSITIFFTGMVMALQIGNAMEAQMPGSSIYIGSAVTIAMFRELAPVLT
                     ALLLAGRVGSAIAAQIGTMKVTEQIDALITLSANPIQYLSVPRFISCVIMTPSLTVIS
                     GFIGVLGGGVIGYFGLGISPEQYFSNVVKYTTAMDFLSGIIKSVFFGIEISIISTYEG
                     FKTTGGAEGVGQSTITAVVKSSMVILISDYFLTYLLQLVG"
     gene            complement(4607..5935)
                     /gene="dnaB"
                     /locus_tag="P5123_07705"
     CDS             complement(4607..5935)
                     /gene="dnaB"
                     /locus_tag="P5123_07705"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: protein motif:HMM:NF004384.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="replicative DNA helicase"
                     /protein_id="HRX16182.1"
                     /translation="MQQVPPHDLEAEASCIAAITISRDALVKVLDLLSPDDFYLERHK
                     IIFEAVTDLDRSNSPVDLLTVKQRLQDTSLFDRAGGDAYLAELYQTVSTSANAEYYAK
                     RIKELSLRRMLITVSRDVAVRCHDMTIDTPTMLDEAERDIFRVTERRITSDIETIDEI
                     VRQTLDQINLLHQNKRAVTGISCGFTDIDKMLTGFHESELIILAARPSMGKTALALNF
                     MNHIVLEQKKPVFFFSLEMPSTHLLQRLICIEGLIDAQRLRTGHLNNDEMRTINQVAE
                     KYRNAPILIDDTPGVTIADIRAKARRATQKQSLGMIIIDYIQLITSTSRVDRQQQVAE
                     ISRGLKLLARELNCPIIALSQLSRAVESRTDQKPILSDLRESGSIEQDADVVMFIFRE
                     DRVKRDSERTGRADIIVAKQRNGPIGDVELLFWDKFTKFNDVDIVHTYPD"
     gene            complement(5942..6385)
                     /gene="rplI"
                     /locus_tag="P5123_07710"
     CDS             complement(5942..6385)
                     /gene="rplI"
                     /locus_tag="P5123_07710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017851930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L9"
                     /protein_id="HRX16183.1"
                     /translation="MKVILQKDVANLGDAGDVKIVADGYARNFLIPKKMVIKFNESSK
                     AAIEQQKHLIKVKKEKRKKISASVSDKLKELSLVFKAKVGEEDKLFGAVTSLDIAKKL
                     SEAGFEIDKRKIELEHPIRKTGEHTVSIKLDEGITSEVKVLVEKE"
     gene            complement(6468..7343)
                     /locus_tag="P5123_07715"
     CDS             complement(6468..7343)
                     /locus_tag="P5123_07715"
                     /inference="COORDINATES: protein motif:HMM:NF021506.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2232 domain-containing protein"
                     /protein_id="HRX16184.1"
                     /translation="MLSGFVIIIGTMLIAGLSFPVYKWGYKTLFIIAALCIASVIAFA
                     DSFVNLSTALTLLTIAAAAGITFRYKFSVQFYIITASVSLAVMLSIHYYFLRFYSGRD
                     VFTELIKQITISMSQVEDEILQANIKAATTVFTSIKDYHIEPYYFFVNSFIMTLLSYF
                     FLRIIYTFYIIRNIIIKGVEFFKLDERIVLPLIAFFAGFLFVKPGTSEAVYTISVNGL
                     LILATLYFIQSIGIIRHFLIRKKLPLIILPLGLGLTIMFNPLYFPVVALILSGLGLLD
                     VWTDFRGFNRQTEEK"
     gene            complement(7353..7745)
                     /gene="rpsR"
                     /locus_tag="P5123_07720"
     CDS             complement(7353..7745)
                     /gene="rpsR"
                     /locus_tag="P5123_07720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016525425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S18"
                     /protein_id="HRX16185.1"
                     /translation="MSEEIKNNEETSSETEAVATSAPTTANTEDSESSESRPSAARND
                     QRGDRSAQGDRKQRRVPRFRKKVCKFCHNKEIAIDYKKPDVLERFLTERGKILPRRIT
                     GTCAKHQRRIATEVKRARMIALLPFVEL"
     gene            complement(7816..8268)
                     /gene="ssb"
                     /locus_tag="P5123_07725"
     CDS             complement(7816..8268)
                     /gene="ssb"
                     /locus_tag="P5123_07725"
                     /inference="COORDINATES: protein motif:HMM:TIGR00621.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003697 - single-stranded DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HRX16186.1"
                     /translation="MASSDINRVNLIGRLTRDPEIRQIPSGTSITTFSIANNRIYVVN
                     NERREEVSYFNCLIWGKAAEALAKYAKKGQRIAVDGRIQQRRWQDNEGNNRSVVEVVA
                     ENFQFLSSSQDNQGSDSYSGPEANQGAGQYPQNDGASETFLSDDDIPF"
     gene            complement(8369..8656)
                     /gene="rpsF"
                     /locus_tag="P5123_07730"
     CDS             complement(8369..8656)
                     /gene="rpsF"
                     /locus_tag="P5123_07730"
                     /inference="COORDINATES: protein motif:HMM:TIGR00166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S6"
                     /protein_id="HRX16187.1"
                     /translation="MRTYELTTIVRSNRLEEGKEAFAAILAKHGVTIKSDEDWGQKKL
                     AYAIDDQKEAYYIFKIVQTEPETVEKVTNEIRLNDVFLRSMFVVVDEKQAS"
     gene            8971..>9320
                     /locus_tag="P5123_07735"
     CDS             8971..>9320
                     /locus_tag="P5123_07735"
                     /inference="COORDINATES: protein motif:HMM:NF024857.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043856 - anti-sigma factor antagonist
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="STAS domain-containing protein"
                     /protein_id="HRX16188.1"
                     /translation="MSGNYDVRFEMIGDVPVIYISGDMTSDADSAVVKIFREIKAKAG
                     VAKVIFNFSETNYINSAGLATLINVIQEMEKVEGRVVFAGLTTHFQKVMDIVGITEFV
                     KISDTNEGAVALLE"
BASE COUNT         2657 a         1723 c         1997 g         2943 t
ORIGIN      
        1 gatccggtta ccggttcgac tgttaatcat gtaggtatat atcttggcaa ggataagttt
       61 gtgcatatgc cgggagaagg tgcttatgtg cgtattgaaa ggctggttaa agattccaga
      121 tggagcgcaa gattcgcagg ggtaagatcg tatcataagc tttttgatga ttttaccgaa
      181 aagtcatcaa agtcaccgga taaaaaagag aaggttcagg cttttaagaa catggatccg
      241 gatgttcagt tcgagacatc agacatgtga atgctaaatt atgcgagtta tcagaattga
      301 aagatcgtct tcaggttctt tgctccctgt aaactggtat actttttgaa gaaccagttc
      361 tgaaagttca tccggattga gttcgctgtt ttgacgcagt atttccatta gacgagattt
      421 tccgagaaat tcatcttcac tgttttttga ttcaactatt ccatcagtgt acaaaacaag
      481 aatatcaccg gaatgaatcg ggcaagagct tgatacatac tcctcttcag ggaacatgcc
      541 cactataggg ttcaatgaac cgagttcggc aaaggagccg tccttgtgca ttagtagtac
      601 ttcgttgtgg ccaccattcg aatagatcaa ctcattgatc gagaggtcga ttattccgta
      661 aaaagcggtt agaaacatgc cagcaccgcc gacagttttt aatagagagc tgtttatctc
      721 atcgagaaca taaaaagggg gattatgcgg tttagcaagg ctggcaaagg acattttagc
      781 catggtggta atcagggcag cagaaatacc gtgtccgctg acatctacta tcgcgatacc
      841 cattgagttt tcggtaaaag gaaacacatc gtaatagtct ccacccagat tttgcatagg
      901 gtggtatcgc cctccaattt ggaaaaactg tgattcggga aacttttttg gaattattat
      961 ctgttgaaca gagcgagcca tttgaagttc gcgttcgaat gtgctgtttt taatatttag
     1021 ctcataattt gcgtgtttta gctgttgtgt acggttttcc acaaggtttt cgagctcttg
     1081 attaaggtag atgttttcaa aggctatgga cacattggag cagaatatgt cgatcatatc
     1141 gcgctcccac tcttgaagag cctcatcccg ttccagatat atgatatttt cgctgttgtt
     1201 ctgagtcttg aaataggcta tgtagtggta atcatcaaag acattgcgtt tttcgttaaa
     1261 agctatctct agaagttgat actcttcagc tggcagaaca tctttggctc ttgtatttgg
     1321 tcgactattc tggtattttc cggttgcggc caaaataacg gggtgtccgt ccacatttat
     1381 agcggttagg ccggaggtct ggcaataaaa cgcgttattg ctgtatcgta gtatagatgt
     1441 gaactgatca agtatgccag aaacgaagtt ttttaaggat tgaacctcaa aaatatgtgc
     1501 tgtagcatca attatttttt caagggcacg cttgcttttt tcaattgtta tgatgtctct
     1561 gtatgatctt aaggcggtga ttatagtgct gaaaagtcgt tctgtagtaa gttctgtttt
     1621 tgatttatag tcgtttatat cgtattcaac aattacacgt ttttcaggtg cctgacccgg
     1681 ttgaccagtc cgtaaaatta ttctgataag gctgttttga agctcttccc tgatatattg
     1741 tgttaaaagg agtccggaat tttcttgttc cataacaaca tcgagcagaa taaccgcaat
     1801 atcgtccctt tcgttaagaa tctgacgtgc ctcggcatcc gaataggcac ttaaaaactc
     1861 gaccgatcgt ccggaaaatt ccagatctga cagaacaagt tttgtaacct tgtgaacatc
     1921 ctgctcgtca tctacaataa gaatcttcca tgtttcacgg agcttttttt cgtaaatttc
     1981 ttcctgacta tcgtcaaaaa aaacaagatc gtctgcgttt tgtctggttt tcagaggtac
     2041 acctcgtata gcccataatt gatattttaa caaacaggta taagataata aacacgtcaa
     2101 gaaattgcaa ctaattcatt actggagatg tgtaaagttt ataatttgct taagaagatt
     2161 tattattgca tctttttggg tcgattaata ccttataagt gttaaataga gtggataaaa
     2221 cttaaaaagt aaatctgttt gacaaagggg gtaatattgt acattttttt gtgcaaaaca
     2281 ttatggaaag cggttatatg gcaaaagaag attaaatgac tagaatattg atcgtttttt
     2341 gatttcgtga tcctaaatgg atcctaagga gattttatga agaaaattat ctattttata
     2401 gctatgatgt cagttttttc tgggttttcg gtgaacgccc ttgcgggaaa tgttacagaa
     2461 ctttcaatgg gggcaacaca ggcagacgca gtaggtgacg attgggggct taatgtgagt
     2521 gcggcatggt ggaatgagct aaactatatg tttgcattgg gctttaatcc tcagtttgtc
     2581 tggtttcagt gggacaagta tgttactgat aataacggtg acaaaatata tcaggaaaaa
     2641 gaggttggtg gagttgtaga tgagtatccg attgttgaac aggcaaacgc ataccttata
     2701 cctcttcata tcgtagctaa agttcagttt cctctaagtg acactgtgac tccttatcta
     2761 aaagggggag gcggatatac ttttatgccg ttgacctatt cggatgccga ttccgagatg
     2821 tttggtggcg ctacatggac agcagctgtt ggatgtcagt ttagggttcc tgatatacca
     2881 aattttaggt tctttgcaga gtttggatat cgaggttcga aacttactaa tgatgataac
     2941 gtccaaatag acatgagtgg tgtagttatt catgcaggtg ttcaatatgg actgacgcga
     3001 gacaagcctc gctcggcaac tacagatagc ggaattgtat ggtaaaaaga ataccgcctt
     3061 atgagaacat aaggcggtat ttctttctaa gcatcatgaa atagtcaaga taatcgtctt
     3121 ttttatagtc ggggtaggtc cagtcaaaaa attcaaattt ccctttggtg aaatagtact
     3181 cgttttccag ataaacgccc ctgtctacat acatcctatg ataatactcc ttgcaacttg
     3241 caagaaaaac attcgaaagg gttaagtatc caggatcaat gtttattttg cgtgtttcat
     3301 gagacgatga aagtttttct tcaaggttgt tggtgaatat cttgatttcg gcgatcatgt
     3361 ttcgatcaac cagctcggta aacgagaaga atattttgta caggttttca cctatactgt
     3421 tgtaatatga agaaaggtcg aagggaatct tttgagttat agtatcaatt tttccataac
     3481 gggcgcccat ctccttaagt gtcgacaaaa caagctgatc agaagatgtt aacactccag
     3541 caaaaagaag agcttttgtg ggaataatag cttttgccat tttaaccgac cagctgtaaa
     3601 agataggtta aaaaatagtc ggaaatgagt attaccattg aagacttaac cacagcagtt
     3661 attgtagatt ggccgacgcc ttctgcgcca ccagttgttt tgaagccttc ataagttgaa
     3721 attatggata tctcgatacc aaaaaaaaca gattttatta tacctgaaag aaagtccatt
     3781 gcagttgtat atttaacaac atttgagaag tactgctcgg gagaaatgcc cagtccaaaa
     3841 taaccaatta ctcctccacc aagaacaccg ataaatccgg atataacagt cagggatgga
     3901 gtcattatga cacatgaaat aaagcggggt acagaaagat attgtatggg gtttgcggat
     3961 aaagtaatta atgcgtcaat ctgttctgtc actttcattg tgcctatctg cgcggcaatt
     4021 gccgagccta cacggccggc cagaagtagg gctgtaagta caggggcaag ttcacgaaac
     4081 atggcaattg ttacagctga accgatatag attgaagatc cgggcatctg ggcttccata
     4141 gcgttaccaa tttgaagagc cataaccatc cccgtaaaaa aaatcgttat ggatgtaact
     4201 ggaatggatt tgtatcctat ttcgagaagg ttctgtataa gatttttgaa atcaaacgga
     4261 ggtttaaata tccatcttac agtttctttg aagaatatgg catagttgcc gaacccttca
     4321 aaaaactggt tactttttct tataaaggat aagatcacgc gataacctcg tatattatgt
     4381 taggtgtagc tgatagtttg attttggcga tcaagccaat aacccaaaag ctacaaaccg
     4441 ttcgatacta aatagggtta tttcttgata atgatcgaga tatttcaaga ataaaaaagg
     4501 ttgtgaccta ggactaaaaa tgagttgtga caggctatga tggataaaaa gtaatttcct
     4561 gtttatcgtt tttatcgttg tattgcgata gataaaaaga tgattcttaa tccggatatg
     4621 tatgaacaat gtcaacatcg ttaaacttgg taaatttgtc ccagaagagt agctctacat
     4681 cgccgattgg accattacgc tgtttcgcga ctataatatc ggcacgcccg gttctttcag
     4741 aatcgcgttt tacccggtct tcacggaata taaacataac aacgtcggcg tcctgctcta
     4801 ttgatcccga ttcacgaaga tccgaaagta tcggcttttg atcggtacga gactcaactg
     4861 cgcgcgataa ctgcgacaag gctataatag ggcagttcag ttcccgcgct agaagtttca
     4921 atccccggga tatttcggca acctgctgtt gtctgtcgac gcgtgaagtc gaggtgataa
     4981 gctgaatata gtctatgatt atcattccga gggactgctt ttgagttgct cttcgggctt
     5041 tggcgcgtat atcggctatg gtaacacccg gagtatcatc aataagaatt ggtgcatttc
     5101 ggtatttttc ggctacctgg tttatggttc tcatctcgtc attattaaga tgaccggttc
     5161 ttagacgttg agcatcgata agaccttcga tacagattag acgctgcaaa agatgggtag
     5221 aaggcatctc gagagagaag aaaaaaaccg gttttttctg ttcgagtact atatgattca
     5281 taaaatttaa tgccagcgct gtttttccca ttgatggtcg tgctgcaagt attattagct
     5341 ctgattcgtg aaatccggta agcattttgt ctatatcggt aaaaccgcaa gatatccctg
     5401 ttacagctcg tttgttttga tgcagaaggt taatctggtc aagagtttgt ctgactattt
     5461 cgtctatggt ttcgatatcg gaggtgatgc gtctttcggt tactctgaag atatcacgtt
     5521 ctgcctcgtc aagcatggtt ggagtgtcta ttgtcatgtc gtggcaacga accgctacat
     5581 cacgagatac tgttattagc attcttctta gtgacagctc tttgatgcgt ttggcataat
     5641 attcggcatt ggccgaagtc gaaaccgtct gataaagttc tgcaagatag gcgtctccgc
     5701 cggcgcgatc gaaaagagat gtatcttgta gtctctgttt tacggtaaga agatcaaccg
     5761 ggctgttaga acgatcaagg tcggtaacgg cttcgaaaat tatcttgtgc cgttccaggt
     5821 aaaaatcatc aggggagaga aggtcaagaa ccttgaccaa cgcatccctg ctgattgtta
     5881 tcgcagcgat acacgacgct tcggcttcca gatcatgagg aggtacctgc tgcataaaaa
     5941 attactcttt ttccactaga actttaactt cagaggtgat accctcgtca agtttgatgg
     6001 ataccgtatg ttctccggtt ttgcgaatcg gatgttccaa ctcgattttg cgtttatcta
     6061 tttcgaaacc ggcttcagat agtttcttgg caatatcaag agatgttact gctccgaaaa
     6121 gtttgtcctc ttcgccaacc ttggctttaa ataccagaga aagctcttta agcttatctg
     6181 aaacagaggc actaattttt tttctttttt ctttttttac tttaatcagg tgtttctgct
     6241 gttcaatagc agctttgctg gattcattga actttattac catttttttt ggtataagaa
     6301 agttgcgggc gtatccatca gcgacaatct taacgtctcc ggcatcgcca aggtttgcaa
     6361 catccttttg aagtattact ttcatttttt tctcctgcct aaaattatta ggtatttaaa
     6421 agggtgtata atgcttatac accacaaaac atttttaaaa caataatcta tttttcttca
     6481 gtttgtctgt taaatcctct gaaatcggtc catacgtcaa gtaatccaag ccctgatagt
     6541 ataagtgcta caaccgggaa atagagagga ttgaacataa tggttaaccc tagtcccaaa
     6601 ggaagaatga tcagcggcaa cttctttctt atcaagaaat gcctgattat ccctatagac
     6661 tgaataaaat agagagtagc tagtattaac agaccattta cggaaatggt gtaaaccgct
     6721 tcggatgttc caggtttaac aaataaaaaa ccggcaaaaa acgcaatcag cggcaaaaca
     6781 attcgctcgt ctagcttgaa aaactcgacc ccttttatta ttatatttcg gataatatag
     6841 aaggtatata taatccgtaa gaaaaaatag gacaaaagag tcattataaa actgttaaca
     6901 aagaaataat agggttctat atgataatcc ttaatacttg tgaaaacagt tgttgccgct
     6961 ttgatgttgg cctgtaaaat ttcatcctcg acctgggaca tgcttattgt tatctgtttg
     7021 ataagctcgg taaaaacatc tcttccgctg taaaacctta aaaagtagta gtgaatgcta
     7081 agcataacag caagcgaaac agaggctgtt ataatgtaaa attgcacaga aaatttgtac
     7141 ctaaacgtaa tccctgccgc tgcagcaatg gttaaaagtg taagtgcagt cgacaagttg
     7201 acaaacgagt cggcaaacgc aattaccgag gcaatacaaa gagcagcaat gataaacaga
     7261 gtcttgtatc cccatttgta aacaggaaac gagagacccg caatcagcat ggttcctata
     7321 attattacaa atccgcttaa cattataccg acttatagct cgacgaaagg taaaagtgct
     7381 atcattcgcg cacgctttac ttcggttgcg atacggcgct ggtgttttgc acaggttccc
     7441 gtgattcgtc ttggcaaaat ttttccgcgt tctgtaagga agcgctcaag aacatcaggc
     7501 ttcttgtagt cgatggcgat ctctttgttg tgacaaaatt tgcacacttt ttttctgaat
     7561 cgaggaactc ggcgttgttt gcgatctccc tgtgccgacc tgtctccacg ttgatcattt
     7621 cttgcagccg atggtcttga ttcactgctt tcgctgtctt cagtattggc agtagtgggt
     7681 gctgaagtgg caactgcctc ggtctcgctt gaagtctctt cgttattttt tatctcttcg
     7741 gacatttttt tctccttgtt ctgctaaaca cgggtcagac ccgcaataat gggcgaatac
     7801 gccgcagtaa gttgtttaaa acggaatatc gtcatcactt aagaatgttt cggatgctcc
     7861 atcgttttga gggtattgac cggctccctg atttgcctct ggtcccgaat agctatcaga
     7921 accttgatta tcctgactag aggataaaaa ctggaagttt tccgcaacta cttcaacaac
     7981 agaacggttg ttgccttcgt tgtcctgcca tcttcgttgt tggattcttc catcgactgc
     8041 tattctttga ccctttttgg catatttagc tagggcttcg gccgcttttc cccagatcaa
     8101 gcaattgaaa tatgaaactt cctctctgcg ttcgttgtta acaacataaa tccggttatt
     8161 agctatggaa aatgttgtaa ttgatgtgcc gcttggtatc tggcgaattt ccggatcacg
     8221 ggtaagtctt ccgattagat taaccctatt aatatcagat gatgccatag aaacccctca
     8281 tttgcatatt tgtttgataa ccagcatgat atcaggtttt tattatataa aaagctgata
     8341 gtcagaggtt gtaatgatca tgattatttt atgaagcctg tttttcgtcg actacaacaa
     8401 acatagatct tagaaaaaca tcgttcaatc tgatttcgtt tgttactttt tcgactgttt
     8461 cgggctcggt ctgaacaatc ttgaagatgt agtaggcttc tttttgatcg tcaatcgcgt
     8521 aggccagttt tttttgaccc cagtcttcat cagatttgat agttacgccg tgtttggcaa
     8581 gtatagcggc aaaagcttct ttaccctctt caagccggtt tgagcgtact attgtggtaa
     8641 gttcgtaagt tctcatcgtt cctccttcct gtggaatatt tgctaaaata cagtatgttt
     8701 ttagcagaaa ggcatatttc aaaacaatag ttgaattatg aggtaaaatg agcaacaata
     8761 attttcataa attaaacagg ttttgatttt ttttgttctt atcgaagttt tttagttatc
     8821 agaataatgt attttgccta ttatttcgct tgacgatata aaaaaagtat tatcaagtcg
     8881 atgagtatag cgttttttat gcgtttcaaa ttctaagctg tctgtgacta tttttataat
     8941 gtcgaagacc agtgctttag gaaaaatata atgagtggaa actatgatgt tcgtttcgaa
     9001 atgataggtg atgtgccggt tatttatatc tcgggagata tgacttcgga tgctgatagt
     9061 gccgttgtga agatttttag agagataaag gcaaaagccg gtgttgcaaa ggtgatcttc
     9121 aatttttcag aaaccaatta tataaattcg gccggtcttg ctactctgat caatgtaatt
     9181 caggagatgg aaaaggtgga gggtcgggtt gtgtttgcag gattaaccac ccactttcaa
     9241 aaagttatgg atatagtcgg cataactgaa tttgtgaaga tttccgatac gaacgagggt
     9301 gccgtagctc tgcttgaaga
//
LOCUS       DAOTGZ010000099         9289 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_3064
            NODE_8855_length_9289_cov_4.900260, whole genome shotgun sequence.
ACCESSION   DAOTGZ010000099 DAOTGZ010000000
VERSION     DAOTGZ010000099.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868088
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 9289)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9289)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:22:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,785
            CDSs (total)                      :: 2,751
            Genes (coding)                    :: 2,732
            CDSs (with protein)               :: 2,732
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 12 of 19
            Pseudo Genes (internal stop)      :: 5 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9289
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8855_length_9289_cov_4.900260"
                     /isolate="AMR_MDS_3064"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..883
                     /gene="gguB"
                     /locus_tag="P5123_07740"
     CDS             <1..883
                     /gene="gguB"
                     /locus_tag="P5123_07740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013607642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HRX16189.1"
                     /translation="AGVAIGAWQGFWIAYVGIPPFITTLAGFLYWRGFAHLILNGLTI
                     SPFPDNYLKYFTSFLPTSKEVLLSPEEAEFVKMVTIAVGAIICLLFVAYLIFDRKTKT
                     KKGYDVDSWMAIGAKIAIIGGGVMYASWLLANHKGIPVVLILLAVIVLSYSYFTTKMV
                     PGRHFYALGGNEKATRLSGIDTKKIMFFAYTNMGFLSAVAGLVVAGRFNSANPAAGNL
                     YELDAIGACFIGGASAYGGTGTVGGAVIGAMFMGVLNNGMSYLGLDANWQKTVKGLVV
                     LAAVVFDVISKKDKKVT"
     gene            complement(1110..2027)
                     /gene="dusB"
                     /locus_tag="P5123_07745"
     CDS             complement(1110..2027)
                     /gene="dusB"
                     /locus_tag="P5123_07745"
                     /EC_number="1.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR00737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0017150 - tRNA dihydrouridine synthase
                     activity [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA dihydrouridine synthase DusB"
                     /protein_id="HRX16190.1"
                     /translation="MISDTLPFTSPIVLAPIAGYTDYPFRLLCKEQGAGMVYTELISA
                     EALCRNSKKTCQLAALFPDEFPAAVQLFGSSPDRMAEAAEIICKMLPKPALIDINSGC
                     CARKICNNNSGAALMKDPNLIFNIVSQIKSVSDVPVTLKIRLGFTQNTKNYALVIEKA
                     ALAGASAVTIHGRTSDQQYSGEANWNEIEKAAQLSPIPVIGNGDISSYKNAIDRLEST
                     SCSGIMIARGAIGNPWIFSGFEPSDKERFETAARHAQMMFNHYGDHGIIMARKHMVKY
                     FHHKENSSQLRQKLVTANTIDELMHILNP"
     gene            complement(2017..2982)
                     /locus_tag="P5123_07750"
     CDS             complement(2017..2982)
                     /locus_tag="P5123_07750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002775853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HRX16191.1"
                     /translation="MKIELWGVRGSLPTPLTNNEYQLRVRSIIEATLKNGSSDATIDD
                     IIGLLPPHVSTLYGGNTTCVTVTSKKGNLVILDAGTGLLPLGDKLITREAGEGRLTAN
                     FFFTHTHWDHIQGLPFFKPLYIKGNRFIFNSPIKDLQERLNYQQKFKFFPREFDDTDS
                     IKEFNQLKANESYKVDDSLIVDFIPLRHPGGSFAYRFREGEKNFIFSTDVEFRGNAVE
                     SFTPDHGDFFCAADLLILDSQYTLDEAFAKFDWGHTSYSMAVNCGINWKVKKLVLTHH
                     EPSYSDEKLASIYHDAKEHMDASAKAAQIEVPELFLATEGMVFDI"
     gene            3149..3982
                     /gene="asd"
                     /locus_tag="P5123_07755"
     CDS             3149..3982
                     /gene="asd"
                     /locus_tag="P5123_07755"
                     /EC_number="4.1.1.65"
                     /inference="COORDINATES: protein motif:HMM:TIGR00163.1"
                     /note="Phosphatidylserine decarboxylase is synthesized as
                     a single chain precursor. Generation of the pyruvoyl
                     active site from a Ser is coupled to cleavage of a Gly-Ser
                     bond between the larger (beta) and smaller (alpha chains).
                     It is an integral membrane protein; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0004609 - phosphatidylserine decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="archaetidylserine decarboxylase"
                     /protein_id="HRX16192.1"
                     /translation="MSVFLFKLLPKAMISRLVGRLTLTRFSKLFIDWFVKKYGVNTEE
                     IEYPEKGFTSFNHFFTRTLKNGVHSIDKEKNCVVSPVDGRIDEFGIITGTRIMQAKNL
                     DYLVSDLIPSDCHHLFIDGFFITLYLSPADYHRIHSPINGKINELIYAPGKLFPVKEI
                     ITRSISRVFARNERMISLIENEFGRCAVCKIGAMNVGRIAVNFDTIETNRSMFRRGAR
                     KVYGNMAPLVHRGDEVARFNMGSTVILLFEKDMFAPAQLRKGDHVRMGQKLGLLHMKE
                     K"
     gene            4201..5055
                     /locus_tag="P5123_07760"
     CDS             4201..5055
                     /locus_tag="P5123_07760"
                     /inference="COORDINATES: protein motif:HMM:NF024299.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HRX16193.1"
                     /translation="MRQSLSVPSEAIDVYNKALNFSNKGDYSIALTEYKKAISIYPQF
                     LEAYNNMGEIYSKLGDTSRAIDVYREALSISLNDRVLLNIGVEYYNQKEHDKALEYFK
                     KSISLNPDFLESHFYAAMAYFNKEDYNSAVTHFQKVISFDERHLKANYLLSYIYYDWK
                     QYQNAVKCLERILDIADDKQFVNRYYGFCMYHLGNYDKAVQYLQVALKENPAYSRFKD
                     YLSSLTYENKIKELGDIKQKIKELESRIMQKDTKPSLHEYSKLSMLYIFNGEYKKAED
                     LLTSVKIQ"
     gene            5189..5710
                     /locus_tag="P5123_07765"
     CDS             5189..5710
                     /locus_tag="P5123_07765"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRX16194.1"
                     /translation="MNTINSIFKIIMFFFCFMFFCCSSSSLQEAPVGTALVFFHVTLE
                     GITDADIVVLDEKNSEIKTKVRNGDTVVPMFLIPGRTYSIQEVRFGSNKLLLKKPATF
                     KGAVPGVINYAMTIDVVRSGFMQRFKVYSSEEHYEKAKSKFNNRYRDYSKSKSFNRLV
                     TENTVEKSKLIDF"
     gene            complement(5764..6570)
                     /gene="tatC"
                     /locus_tag="P5123_07770"
     CDS             complement(5764..6570)
                     /gene="tatC"
                     /locus_tag="P5123_07770"
                     /inference="COORDINATES: protein motif:HMM:TIGR00945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="twin-arginine translocase subunit TatC"
                     /protein_id="HRX16195.1"
                     /translation="MKLNRLEQRISRINQENYDNEAELRGDTPISYSEHLGELRIRLF
                     FVLIFIVIFFALGLYLNNYIHDFLIEPVKAYKNLFLLKYDIMGESIIIQLKLALGFSL
                     SVCIPIAVFQFWRFIRPAISPQNRMFIRLSLLFAVALFYTGAVFCFIVIIPFTIDMII
                     KFAHIYVQTNINMSSYFGFLILLSASVGIVFELPVAVSILTKLGIISPTLLVSKRKYA
                     IVSIWIFSALVTPQDILTQAILAIPLMLLYEFSIFVSFLIFRKRRQTINK"
     gene            6957..7514
                     /locus_tag="P5123_07775"
     CDS             6957..7514
                     /locus_tag="P5123_07775"
                     /inference="COORDINATES: protein motif:HMM:NF013073.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="C40 family peptidase"
                     /protein_id="HRX16196.1"
                     /translation="MKKKTKNYNLVLLFIIFVILTGCTPPPGTRFSGVQKVRDRSYSK
                     SSISDHFVSQRQDGKFVSGETNAIVRRAKSCIGVPYRYGGTDRRGFDCSGLVLYSYQP
                     AGVSLPRSAKSQFKSGKRITLSQAKPGDLLFFKTNRSTISHVGIFIGNGSFVHAPSKG
                     KNVRIDKLSNPYWKKVFAGSATYLR"
     gene            7557..8591
                     /locus_tag="P5123_07780"
     CDS             7557..8591
                     /locus_tag="P5123_07780"
                     /inference="COORDINATES: protein motif:HMM:NF015087.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0016051 - carbohydrate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylneuraminate synthase family protein"
                     /protein_id="HRX16197.1"
                     /translation="MKKLSEIFGDVPVICVAEIGINHNGNVDLAKKMITAAAKSGAHA
                     AKIQSIDASELYSTYSKSISNGDKKYNKDESIIDFFKKFYLNEEEHRLLFKHARSENI
                     ELFSTPFDNDTVDMLEDCGASLYKIASCDVTNHKLIKHISSKRKPIILSTGMSSDNQI
                     KQAVRIIKSAGNDFILLHCVSLYPVEPINMNLRRIDVLKSMHKCKVGLSDHSNEEVVL
                     AGAVFKGISMVERHFTDEYRDDCPDKEVSYDSQKFRSMVDNIGYLLEIDGCGNDLIST
                     DEKAVAKASRRSLFISTDLKKGTVLTGDMLKCKRPFAGICASLYYDVIGKKLTKNISS
                     DMPLYPEDIE"
     gene            8672..9262
                     /locus_tag="P5123_07785"
     CDS             8672..9262
                     /locus_tag="P5123_07785"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRX16198.1"
                     /translation="MKKALITILLLSSCSYFMVRSNNYYTKDEAKILEQSSNHLGMSF
                     GYDSRIDLNYFYKMKYGSEKIGVFNRGLSATKDNTQGKPEETPDAQPNIEAFRKSLSQ
                     YTVQEQFYLLVKVYYLYERTLYYKKVMSMQKKWRDFNTVNLEYLPATELYFTEFENYF
                     KETQPKLYSDFLAQKNDLKVDAITELQKENTVVDEY"
BASE COUNT         2983 a         1448 c         1841 g         3017 t
ORIGIN      
        1 tgccggtgtt gctatcggcg cgtggcaagg attctggata gcatatgtgg gtattccacc
       61 ttttattacc actcttgccg gctttttata ctggagaggt tttgctcatc ttattcttaa
      121 cggtttaacc atttcaccgt ttcctgataa ctatcttaaa tattttacca gttttttacc
      181 tacctccaaa gaggttcttt tgtctccaga agaggcggaa tttgtcaaga tggttacaat
      241 tgcggttggc gcgattatat gtttgttatt tgttgcatat ctgatatttg acaggaaaac
      301 aaagacaaaa aaaggatacg atgtggatag ttggatggca atcggggcaa agattgcaat
      361 tatcggcggt ggtgttatgt acgcttcatg gttgctggct aatcataagg gtattcctgt
      421 tgtattgatt cttttggccg taattgtact tagttacagc tattttacta ccaagatggt
      481 accaggacga catttttacg cccttggcgg aaatgaaaaa gcaacacgtc tttcaggtat
      541 tgatacaaag aagattatgt tttttgcata cacaaatatg ggattcttat ccgcagttgc
      601 cggacttgtt gtagcaggac gttttaattc ggccaatcct gccgccggta atctttatga
      661 attggatgcc attggggcat gttttatcgg tggagcttca gcttacgggg gaacaggtac
      721 tgtaggtgga gctgttattg gagcaatgtt catgggtgta ttaaataacg gtatgtctta
      781 tcttggtctt gatgccaact ggcaaaaaac tgtaaaaggt ctggttgtgc ttgcggctgt
      841 tgtatttgat gttatttcga aaaaagataa aaaagtgacg taaacaaagc cttttctttt
      901 tcactcctat taaacttaaa gccgggatct tttatgtatt ggaagatccc ggttttgttt
      961 tgtgaacata tacagacaca tcaatttggt tagtaaccag atccagattg ttagagaaaa
     1021 atatagggat aaaagcagaa cctcatatag ccctatcgca gtttcctaat aaaatatcag
     1081 aaaacgcgca aaatgaataa tcaacggggt tacgggttaa gaatatgcat aagctcatca
     1141 attgtattag cagtaacaag tttttgtcgc agctgcgatg aattttcttt atgatgaaag
     1201 tatttaacca tgtgttttct tgccatgatg attccatggt caccatagtg attgaacatc
     1261 atttgtgcgt gtctggcagc agtttcaaag cgctctttat cagatggctc aaacccggaa
     1321 aatatccatg gattaccaat tgcgcctcga gcaatcatga ttccgctgca ggaagttgat
     1381 tcaagtcgat ctattgcatt tttgtatgaa gaaatatctc cattaccgat tacaggtatc
     1441 gggcttaatt gtgcagcttt ttcgatttcg ttccagttcg cttctccgga atactgttgg
     1501 tccgaagtac ggccgtgaat tgtgactgca gaggcgcctg ccaaagcggc tttttcaatt
     1561 accaaagcgt aattttttgt attttgagta aatcccaatc taattttcaa tgtaaccggt
     1621 acgtcagata cagattttat ttgtgagaca atattaaaaa taagattcgg gtctttcatt
     1681 aatgcggcac ccgagttgtt attgcaaatt ttacgagcac agcagccaga attaatgtca
     1741 atcaaagctg gtttggggag catcttgcaa attatttctg cagcttcagc catacgatcc
     1801 ggggaacttc cgaaaagttg tacggcagca ggaaattcat cgggaaacag cgctgcaagt
     1861 tggcaggttt ttttgctatt gcggcataat gcctcagcgc ttatcagttc tgtatatacc
     1921 ataccggctc cctgctcctt gcaaaggagg cggaaagggt aatcagtata accggcaatc
     1981 ggggctaaaa caataggtga agtaaaggga agtgtgtcag atatcaaaaa ccataccctc
     2041 agtagcaaga aataattcag gtacttcaat ttgagcggct tttgcgctag cgtccatatg
     2101 ttctttggcg tcatgataaa ttgatgcaag cttttcatca gagtaagacg gttcatggtg
     2161 agtaagtact aactttttaa ctttccagtt tataccgcag ttaactgcca tagagtaaga
     2221 agtatgaccc cagtcaaatt ttgcaaaagc ttcatcaagt gtgtattgtg aatcgagtat
     2281 cagtaagtca gctgcacaaa aaaaatcgcc gtgatcagga gtaaaagact caacagcatt
     2341 gcccctgaac tcgacatcgg tagaaaatat aaagtttttt tcaccttccc ggaatctata
     2401 agcaaaactg ccgccgggat gtctgagcgg aataaaatcg acaatgagag aatcatctac
     2461 tttataggac tcattagcct ttagttgatt aaactctttt atactgtccg tgtcatcgaa
     2521 ttcacgtggg aaaaacttga atttttgctg atagtttaat ctttcctgaa gatcttttat
     2581 gggactgtta aatataaacc tgtttccttt gatgtaaagg ggtttgaaaa atggaagtcc
     2641 ctggatatga tcccaatgtg tatgagtaaa gaaaaaatta gctgtaagcc tgccttcacc
     2701 ggcttctctg gtaataagtt tgtcaccaag aggcagtaaa ccggtaccgg catcaagtat
     2761 tacaagattt cctttttttg atgtaactgt tacacaagta gtattaccgc cgtacaaagt
     2821 tgaaacatga ggaggaagca aaccgataat gtcatcaata gtagcgtcag agctgccatt
     2881 ttttaatgta gcctcaatta tcgagcgtac acgtagctga tattcgttgt tggttaaagg
     2941 agtcggaagt gacccccgta caccccatag ttcaattttc attatatagt tccgtccgcg
     3001 gtctatttgg atgcggtagg taattacgat aattatatgg agttactttg taaagaaaaa
     3061 aataaaaaag gggtttgaca gaagcctcga ttaaagtatc tgttatcttt gtcttataaa
     3121 taatagttgg gttcatggag ggcatttaat gtctgttttt ctattcaaat tattgccaaa
     3181 ggctatgatt tctcgtctgg ttggccggtt gacattaaca cgtttttcaa aactttttat
     3241 agattggttt gtaaaaaaat atggggttaa tacagaagaa attgaatatc ctgaaaaggg
     3301 atttacctct tttaaccact tttttacacg aacacttaaa aatggagttc attccattga
     3361 taaagagaaa aactgtgtag tttcaccggt tgatggtcga attgatgagt ttggaattat
     3421 aactggtacg cgtatcatgc aggcaaaaaa cctggattat cttgtttctg accttattcc
     3481 ttcggattgt catcatctgt ttatcgatgg attttttata acattatatc tttcacctgc
     3541 tgattatcat agaatacatt cacctattaa cggcaaaata aatgagttga tttacgctcc
     3601 aggaaagctt tttcctgtaa aagagattat tacacgttct atttctagag tttttgccag
     3661 aaacgagcgc atgatatcac ttattgaaaa tgaatttgga agatgtgctg tttgcaaaat
     3721 aggcgctatg aacgtgggaa gaatagctgt taattttgat acaattgaaa caaacaggtc
     3781 aatgtttcgc cgtggtgctc ggaaggttta tggtaatatg gcgcctcttg ttcatcgtgg
     3841 agatgaagta gcccgtttca atatggggtc tactgtaata ttgcttttcg aaaaagacat
     3901 gtttgctccg gcgcaattac ggaaaggcga tcacgttaga atggggcaga agctgggtct
     3961 tttgcacatg aaagaaaaat agtaatatat tatctatagg gaacttcgaa aaattaccat
     4021 ttctagaacg gctatgccgt ttaaagccca tgtataaagg aatttttcgc aagttccttg
     4081 ttgaaatgtt aattattaga gattgcctat agagtaaata gttgaggaaa ttcaaaaaat
     4141 aaccgatatt aggtatgtgc tatgagtaaa aaaaatgatc gagcaaatag aggtcagcct
     4201 atgagacagt cactttcggt tccatcagaa gctattgatg tatataataa ggctttgaat
     4261 ttttctaata aaggggatta ttcaatagct ttaactgaat ataaaaaggc aatatcgata
     4321 tatcctcaat ttctggaagc ttataataat atgggcgaaa tttactctaa attgggcgat
     4381 acttcgagag cgattgatgt gtatcgtgaa gctctttcaa tatcgctgaa cgacagggtt
     4441 ctgctcaata ttggagtaga atattataat cagaaagaac acgataaggc gctagagtat
     4501 ttcaaaaaat caatttcgct taaccctgat tttttagaaa gtcatttcta tgctgctatg
     4561 gcatatttta ataaagaaga ctataattcg gctgtaactc actttcaaaa agtaattagt
     4621 ttcgatgagc gtcatttaaa agcaaattat cttctttctt atatttatta cgattggaaa
     4681 cagtatcaaa atgctgttaa gtgtcttgaa cgtattttag atattgcaga cgataaacag
     4741 tttgtgaatc gttattacgg tttttgtatg tatcacttgg gcaattacga caaggctgtt
     4801 caatatttgc aggtagcctt aaaagaaaat cccgcctatt ccagatttaa agattatctt
     4861 tcttctttaa cctacgagaa taaaataaaa gagttgggtg atataaaaca aaaaataaaa
     4921 gagttagaat ctagaataat gcaaaaagat acaaaacctt cattgcacga gtatagcaag
     4981 ctatctatgc tctatatttt taatggtgag tataaaaagg ctgaagactt gttaacttct
     5041 gtaaagattc aatagaatct tatttttttg atagattgag tatcaataaa aaaaagtgtt
     5101 cttgataata ggaacacttt tttattagtg acaattattt tgtttattgt tgttaggatt
     5161 actggatatt aattaaaaag gtgtttatat gaatactatt aattctattt ttaaaattat
     5221 tatgtttttc ttttgcttta tgtttttttg ttgctcttct tcatcgttgc aggaagctcc
     5281 ggttggcact gctttggttt tttttcatgt tactcttgag ggaattacgg atgcagatat
     5341 tgtagttttg gatgaaaaaa atagtgaaat taaaacaaaa gtgcgtaatg gtgatacagt
     5401 tgttccaatg tttctgatac ccggaagaac atattctata caggaagtgc gttttggtag
     5461 taataaatta ttattgaaaa aaccggctac ctttaagggg gctgtgcctg gcgtaataaa
     5521 ttatgcaatg acgatagatg tagttaggtc tggatttatg cagagattta aagtttattc
     5581 gtctgaggaa cactacgaaa aggctaagtc gaagtttaac aatagatata gagattatag
     5641 taaaagtaaa agctttaatc gacttgtaac agaaaatact gttgaaaaaa gcaaattgat
     5701 agatttctaa tagataattg attaaattga aaaataacgt aatgataact gggttttata
     5761 attttactta ttaatggttt gtcttctttt tctgaaaata agaaatgata cgaaaatcga
     5821 aaactcataa agtaacatta acggaattgc aagaattgcc tgtgtaagta tgtcttgtgg
     5881 ggttacgagt gccgaaaaaa tccatattga gacaattgca tattttcttt tgctgactaa
     5941 aagggttggg ctaattatac caagttttgt aagtattgat acagcaactg gcagttcaaa
     6001 tacaatacct accgaagctg acagtagaat taaaaatccg aaatagctgg acatattgat
     6061 atttgtttgc acataaatat gcgcaaattt aattatcata tcgattgtaa aaggaataat
     6121 tacaataaaa cagaaaacag cacctgtgta aaaaagagct actgcaaaaa gaagtgacaa
     6181 acggataaac attctgtttt ggggtgatat ggcgggacga atgaagcgcc agaattgaaa
     6241 tactgcaatg ggaatgcaca ctgataatga aaaacccagg gcaagcttga gttgaattat
     6301 tatgctttct cccataatgt cgtatttaag cagaaacagg tttttataag cttttacagg
     6361 ttctattaaa aagtcatgaa tgtaattatt gagataaagc ccaagtgcaa aaaaaatgac
     6421 aataaaaata agtacaaaaa aaagccggat acgtaattca cctagatgct ctgaatagct
     6481 tataggagtg tctccccgca attcagcttc attatcatag ttttcttgat tgattcgtga
     6541 tattctctgc tccaacctgt ttaatttcat ttattgctcc gcaattttgt gagatatcta
     6601 tatcattttt taaattatga tatttttgta aacagtaagt ttactgtttc ttaaaaaaac
     6661 agtggatatt taaaacctga taaattggga aagaacagct tgacaaatta tgtcgcatgg
     6721 aatgattgtt tatcattatt aattgaggta atatccaatg cttgtaagcg aatgtacgat
     6781 gtgcaatttt cacatttgtg taatgaaaaa ttttattatg tgtggttaca atattgatcg
     6841 caatgaactt cctatagttg tcggagataa tgatcaacag cttgtaagcg gttgcccgaa
     6901 agaaaagtag cagaacgtcc ttaattatca ctttttgttt tccgatttat aatagtatga
     6961 agaagaaaac aaaaaattat aatcttgttc tgctatttat tatctttgta attttgaccg
     7021 gatgtacccc acctccaggt acgcgttttt ctggagtaca gaaggttcgg gaccggtcat
     7081 attctaagag tagtatttct gatcactttg tatctcaaag acaagatggt aagtttgtta
     7141 gtggagagac aaatgcgatt gtaaggcgcg cgaagagctg tattggggta ccttaccgtt
     7201 atggtggaac cgataggcgc ggttttgact gttccggtct tgttttatat tcttatcagc
     7261 ccgcaggagt atctttacct cgtagtgcaa aatcacagtt taaatccggg aaacgaatta
     7321 ccctatctca agcaaagccg ggtgacctgc ttttttttaa aaccaacaga agtactatct
     7381 cgcatgttgg catatttata ggtaatggat catttgtaca tgctccttca aaaggaaaga
     7441 atgtacgtat cgataaatta tctaatccgt actggaagaa ggtttttgcc ggttctgcca
     7501 cgtatctacg ttagaataat tctttacaaa tagtatatcg taactaatca ggtaatatga
     7561 agaaattatc tgaaattttt ggggatgtgc ctgtaatttg tgtagcagaa ataggcataa
     7621 accataatgg aaatgtcgat cttgctaaaa aaatgattac tgcagccgct aagtctggag
     7681 cacatgcggc aaaaattcag tcaatagatg cttctgagct ctattcaact tatagtaaat
     7741 ccatttctaa tggtgataag aaatataata aagatgagtc aataattgat ttttttaaaa
     7801 agttttattt aaatgaagag gagcataggc tgctttttaa acatgctcgt tctgaaaata
     7861 ttgaactttt ttcaacacca tttgacaatg atacagtcga tatgttagaa gactgtgggg
     7921 caagtcttta caaaattgct tcttgtgatg taacaaatca taaattaatt aagcatatat
     7981 cttccaaaag aaaaccaatt attctttcaa caggaatgtc gtcagacaat cagattaaac
     8041 aggcagtacg aattataaaa tctgctggca acgattttat tcttttgcac tgtgtatctc
     8101 tttatcctgt tgagcctata aacatgaacc ttagacgtat tgatgtgctt aagtctatgc
     8161 ataaatgcaa ggttgggctt tcagatcata gcaatgaaga ggtcgtactt gcaggagctg
     8221 tctttaaagg catatccatg gttgaacgac attttactga tgagtataga gatgactgtc
     8281 ccgataaaga agtttcgtat gatagccaaa aattccgttc aatggttgac aatatagggt
     8341 atctgttgga aattgatggt tgtggaaatg atctgatatc cacagatgaa aaggctgtag
     8401 caaaggcgtc aagacgctct ctttttatct ctactgattt aaaaaaaggt acggtattaa
     8461 cgggtgatat gttgaagtgt aaacgtccat ttgctggaat ttgtgcctct ttgtattatg
     8521 atgttatcgg gaaaaaatta acaaaaaata ttagttcaga tatgcctctt tatcctgaag
     8581 atattgagtg ataattgaat tgtttataaa catattgata caattagcgg aaataatcca
     8641 ataaattttc tgaacaatac tggagttcta aatgaaaaaa gcgcttatta ctattttgtt
     8701 gttatcatca tgctcctatt ttatggttag aagcaataat tattacacaa aagatgaagc
     8761 aaaaatactt gaacaaagtt caaatcatct tggaatgagt ttcggttatg attcacgtat
     8821 tgatctaaat tatttttata aaatgaagta cggttctgaa aaaattggag tttttaatcg
     8881 tggtttaagc gctacaaagg ataatactca gggaaaacca gaggaaactc cggatgctca
     8941 accaaatata gaagctttta gaaaaagctt gtcacaatat acagttcagg aacaatttta
     9001 tttgctcgtt aaggtctatt acttatatga aagaactctc tattataaaa aagtcatgtc
     9061 catgcaaaaa aaatggcgtg attttaatac tgtaaatctt gaatatttgc cagctactga
     9121 actatacttt actgaatttg agaattattt taaagagacc caaccaaaat tgtactctga
     9181 ttttttagca caaaaaaacg atctaaaagt tgatgcaatt accgaacttc aaaaagagaa
     9241 tactgttgta gacgaatatt agacatcttg aaataaatat aattatctg
//
LOCUS       DAOTGZ010000100         9250 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_3064
            NODE_8921_length_9250_cov_4.623926, whole genome shotgun sequence.
ACCESSION   DAOTGZ010000100 DAOTGZ010000000
VERSION     DAOTGZ010000100.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868088
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 9250)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9250)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:22:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,785
            CDSs (total)                      :: 2,751
            Genes (coding)                    :: 2,732
            CDSs (with protein)               :: 2,732
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 12 of 19
            Pseudo Genes (internal stop)      :: 5 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9250
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8921_length_9250_cov_4.623926"
                     /isolate="AMR_MDS_3064"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..865)
                     /locus_tag="P5123_07790"
     CDS             complement(<1..865)
                     /locus_tag="P5123_07790"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRX16199.1"
                     /translation="MLAFLPSAMVINSDVGSAVPRHASGFDELYKNRDYKELLSTQNG
                     IFYSYIVLLFNDNPHFINIIMDLQIDSDSNVSYEKLYKLYLSRGKDLPFYKLAVKLYK
                     YYKKPATFDEATQREVEKELESFDIVLRFVSKPDSMMLDYCIYGRKKPIEESHPFLEK
                     TRRIYNIIPLIFYDEFFSSNSTFYSDLIYIDDNEVTNDYNIAVLVREHASTKGLYYIG
                     SEVTNDIKFCLIKASEETADLQDRIKRVFIVHELTHKIINNDYNYYNSIIEEELAIAS
                     TVYADPYLGLTA"
     gene            complement(1015..1368)
                     /locus_tag="P5123_07795"
     CDS             complement(1015..1368)
                     /locus_tag="P5123_07795"
                     /inference="COORDINATES: protein motif:HMM:NF019430.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Spy/CpxP family protein refolding chaperone"
                     /protein_id="HRX16200.1"
                     /translation="MKILKIAVLFLFVAVSSSIVLAQDRPSKSGLSRRSSGSRIECMR
                     EELDLTDAQAEQIEAIKKRFFEEREKFRGDREKMRELADAHRLEIYALLSEEQREKFD
                     KMGRRGFRFRKKDGR"
     gene            complement(1441..1737)
                     /locus_tag="P5123_07800"
     CDS             complement(1441..1737)
                     /locus_tag="P5123_07800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008930304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SET domain-containing protein-lysine
                     N-methyltransferase"
                     /protein_id="HRX16201.1"
                     /translation="MKKTLRVGKSLIHGKGLFATEKICKGDVIGYCDAVPSRKQTDYT
                     LHVEDGAVDVVCCLKYINHNNRPNVVYYDDLSVVALRDIKPGEELTHHYGDDWK"
     gene            complement(1830..2987)
                     /locus_tag="P5123_07805"
     CDS             complement(1830..2987)
                     /locus_tag="P5123_07805"
                     /inference="COORDINATES: protein motif:HMM:NF012428.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate aminotransferase family protein"
                     /protein_id="HRX16202.1"
                     /translation="MRIINSTGHDLKYDNIIRAQNCTLFDDSGNSYLDLESGIWCTPL
                     GHCHPRITRVISEQSAKMIHSGYCYLNPVMDEAASQIFDITGIVDGKCVFLCSGSEAV
                     EYSVKLCRSIFDKPYFLTMKNCYLSAYGISGERPGNEWIEYDWQNDDSIDRIDFSKIA
                     AFVFEPGSSLGLVNFPPKDLVLRIVDRVRDNGGIVIANEVTTGMGRTGKWFGYQHYDI
                     VPDIVAIGKGLGNGYPVSCAAVSDKVLSQFDPHKFHYAQSHQNDPLGATVANEVIRII
                     KEDNLLDRCAQTGVLIYKRLLDIQERYGVIKEIRGRGLMLAIEFMSCDRFSVAEVVND
                     KLLEKNIILTKRPGHEVLRFDPALTIELSDIDYYLDNLENIIRDISRSANL"
     gene            complement(3088..3318)
                     /locus_tag="P5123_07810"
     CDS             complement(3088..3318)
                     /locus_tag="P5123_07810"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRX16203.1"
                     /translation="MYVHKTALDDFLPGFAYKNWVDPFPHTFPEVFLNGKWVSMEATF
                     DKELHEICIQRKLQPMIFAKAQKIMDEKIRAH"
     gene            3478..6177
                     /locus_tag="P5123_07815"
     CDS             3478..6177
                     /locus_tag="P5123_07815"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024831.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HRX16204.1"
                     /translation="MSGTFHQQLKTEMDKIFESEGHKYPQDEILRLDMHCHDKNSDQP
                     DELIGRILNIPETWLPPKSLIKILKKNGSTAFTVTNHNNARSCYQLQDAGYDVLTGAE
                     FTVTVPDFNSRIHVLAYGFTPEEETVLNRKRSNLYKFQEYALSHDIPTVWAHPLFHYK
                     KNSIPDMGLFEKLSLVFQRFETVNGQRDTWQNLLVNQWLDTLTPEIIDQFAKKHKMPV
                     NAFQEDPYTKIRTAGSDSHMGLFAGQTGTILHIPGLQKRLETETASKLALEAIREGRT
                     ALFGSTHDSEKMTVAFIDYFCQIGLNLEDPGLLRILFHKGETKDKLLAMFMTNGFLEL
                     QRHRLTTGFIKTFHKCFLGAPPRSLAKLIIPKDYSPVFDLATSLADVKKMPHAEQPLL
                     YKEKIENIYSHLTDLVIKRLEGKLKNMPQIKDEDMSKTIVELDIPSQLRLLFEKDNSL
                     RSKFTFLRKKPDKSDPFKLDRFLDGLSFPMLASTVITAATFASARVLYNTRPLLNAFA
                     EATGKTPHPKRMLWLTDTFEDRNGIAIVLKSILEEIRRRDLPIDILTCSSTVKPGKNL
                     IVVPPKAEFVLPFYQHQPLRIPDILQLHQIVKDNEYDRLMCSTEGFMGAATLFLKNAY
                     SIPAFFYVHTDWTTFGQKVLNLSSSAQSRVRRLLRAVYQQFDKIFVLNSDQQKWLVSK
                     AMGIPDKNVHLTAHWTDKEYRPQRKSKKQLFGVSEDTPVMLFTGRISHEKGATELPAI
                     YKRINKVIPGVKLVLAGTGPEEEKLKKILPDALFLGWVDHTELPKIYSSADLMILPSI
                     FDTFGCVILEALSCGTPVISYANKGPKDIIIHEKCGFLASNRNKMADYAASYLSSPKR
                     WASFRSAALKRAADYNPTDIFDSLLEDVGLSEQKFKAKNRTTA"
     gene            complement(6188..7447)
                     /locus_tag="P5123_07820"
     CDS             complement(6188..7447)
                     /locus_tag="P5123_07820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020613266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HRX16205.1"
                     /translation="MNNFEVVIIGSGPAGLGAAFGLLEKGCSSVLMIEKRSISSGGLR
                     NDCKQNYTYPVGFPELLWTKDEAENELVTVEKYLNPRITEKKNLEVYKKRAAALGVDL
                     LDIRQAHVGTDKAPALIGSLIQQLEEGGITVWRDTDALAINYEERLVYLKNGKKVGFK
                     YVIAAPGRAGFDWLQDVMKKNGIGFSDNSVDIGIRVECSSENYPIVKDYYDPKFVFPY
                     KIRSFCTNSGAAHVVKEKYEHYYSVNGHSLSQKNRPNNLVNFAMLKTIELTDPVASGQ
                     YFAQVLGQMAMQLAGGQPMMQRVGDFRLGKRSNSKTFNGDLYDFKPTLASATPGDLAL
                     AVPAKILRHIWSSLKMLDTIIPGVLHPSTILYYPEIKTYANRPVFINRFFEVKENMFM
                     VGDGAGTSRGITAAWSSGAKAAKRITR"
     gene            7564..8796
                     /locus_tag="P5123_07825"
     CDS             7564..8796
                     /locus_tag="P5123_07825"
                     /EC_number="3.5.1.28"
                     /inference="COORDINATES: protein motif:HMM:NF013670.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008745 - N-acetylmuramoyl-L-alanine
                     amidase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HRX16206.1"
                     /translation="MHFFKTEKKKIGATKTATRNNGDQIISKPAPITRRLTIIALYLS
                     VWTLLNGSVTSFTIREYTFNKKNYVSLYDIATKTGINQTFDVISQRGKLYHKHHSAVF
                     QAGYAAYLIDGRVYLSDYPVIRKNGEIFIPSLFFREMCSAFFPELEVRLANNHYSLKT
                     KTKTEDPVPFLTPDSPPKEKIAFIFIDPGHGGRDPGAIGGKIREKDLTLKLSRKIRDY
                     LKKNLKGVQILMTRDKDHFIELGKRTEMANRKLKKGTNGLFVSVHVNASISSRSNGHE
                     TYYLSQNPTNEESRATASLENNVVVLESKKHKPYQDVEYVEALMLTTQIQKESALLAD
                     NIQDRMKKNLRETRSRGVKKADFFVLRGALMPAVLVEAGYITNSKERKLLTQKDFQDR
                     IAKSVGDGVIDFIKEYNK"
     gene            complement(8801..9073)
                     /locus_tag="P5123_07830"
     CDS             complement(8801..9073)
                     /locus_tag="P5123_07830"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRX16207.1"
                     /translation="MNISSAVSVVQLSPSQNNRRIYIKAAYDTKNPVSAVKRVGPRRT
                     PVQPEGAIGRGALNRMNEYFRSNHLYGLRGVVSSGPALGRNLDIFA"
BASE COUNT         2903 a         2075 c         1640 g         2632 t
ORIGIN      
        1 ctgccgtaag ccccagataa ggatcggcat atacagtact agcgatggcg agctcttcct
       61 caattattga attgtaatag ttgtaatcat tgtttattat tttgtgagta agctcgtgta
      121 cgataaagac gcgcttaatg cggtcttgca aatcagctgt ttcttcggat gccttaataa
      181 gacaaaattt tatatcgttg gtaacctcgg aacctatgta atagagacct tttgtagaag
      241 catgctctcg cacaagaaca gcaatattgt aatcgttagt gacttcgtta tcatcaatat
      301 aaataagatc agaatagaag gtggagttac tgctgaaaaa ttcatcatag aaaattagag
      361 gaattatatt atatattcgc ctggttttct caagaaaagg atgactctcc tcaatcggtt
      421 tttttcgtcc ataaatacag taatccagca tcatcgagtc aggtttagaa acaaatcgca
      481 aaactatatc gaatgactca agctctttct caacttcccg ctgcgtagcc tcgtcaaaag
      541 tagcaggttt tttatagtat ttgtaaagtt taactgctag tttgtaaaaa ggtaaatcct
      601 tgccacgaga aagataaagt ttataaagtt tttcgtaaga tacgtttgaa tcagaatcta
      661 tttgaagatc cataattata ttgataaaat gaggattgtc gttaaataac agcacaatat
      721 acgaataaaa aataccgttt tgtgtagaaa gcagctcctt ataatcacga tttttataaa
      781 gctcgtcaaa tccagaagca tgccgtggca cagcacttcc cacatcactg tttatcacca
      841 ttgcggatgg taaaaacgca agcataagcg ccaacaaaaa gaaaaatttg atcctggttt
      901 taatacactt accccatttt aaaaatccgg cacttaccgg atttttaaaa tcagctctgt
      961 tgtcaataaa ttcaacaaca atttgttaat tatgaataaa aaggtaagtg ttcgttaacg
     1021 accatctttt ttacgaaaac ggaaaccacg ccgacccatc ttgtcaaatt tctcccgctg
     1081 ttcttcactt aacagagcgt atatctccaa tctatgcgcg tcagcaagct cacgcatctt
     1141 ttcacgatct cccctgaatt tctctcgttc ttcaaaaaaa cgttttttta tagcctctat
     1201 ttgctcggcc tgcgcatcag tcagatcaag ttcttcacgc atgcactcta ttcggctacc
     1261 tgaactgcgc ctgcttagac ccgattttga aggtctatcc tgagccaata caattgaaga
     1321 cgagacggca acaaaaagaa acaaaacggc aatttttaaa atcttcataa tctctcctta
     1381 aaacaataat gattttattc aattgccggt ttgaccctta aatactgtta ttggttcctt
     1441 ttatttccaa tcgtcaccat aatgatgtgt aagttcttcg cctggcttga tatcgcgcaa
     1501 tgctacaact gataagtcat cataatatac gacattaggt cgattattat ggtttatgta
     1561 cttaaggcaa cagacaacat caaccgctcc atcttcgaca tgaagggtat agtctgtctg
     1621 tttacgacta ggaacagcat cacaataacc tatcacatca cccttacata ttttttctgt
     1681 tgcaaaaagc ccttttccgt gtatcaggct tttgccaacc cttaacgttt ttttcattat
     1741 taaactccaa aaccaaacaa gatatgatcg aaaacatcta acagtttacc cctacataga
     1801 tctttaaact cagttctgat aaactaagtt taaagattag cacttcttga aatatccctt
     1861 attatatttt ccaaattatc cagataataa tcgatatcag acaattcaat agttaacgcc
     1921 ggatcaaatc ttaatacctc atgaccggga cgttttgtca aaataatatt tttttcaaga
     1981 agcttatcgt ttaccacttc agcaacagaa aagcggtcgc aactcataaa ttcgattgca
     2041 agcataagcc ccctaccacg aatctccttt atcacaccat atcgctcttg gatatccaag
     2101 agtcttttgt atatcaaaac cccagtttgc gcacatcggt caagtaaatt gtcttctttg
     2161 attattctga ttacttcgtt tgctacagtt gcccctaacg ggtcattttg atgtgactgt
     2221 gcgtagtgaa acttatgcgg atcaaactga gacaaaacct tatcagaaac cgctgcacaa
     2281 cttaccggat agccattgcc aagccctttg cctatggcta caatatcagg tacaatatca
     2341 taatgctgat aaccaaacca ttttcctgtg cgccccattc cggttgtaac ttcatttgca
     2401 attacgatgc caccgttatc cctgactcta tctactatcc gaagtaccag atcttttgga
     2461 ggaaagttaa caagcccaag agagctcccc ggttcaaata caaaagcagc aattttagaa
     2521 aaatcaattc tatctatcga atcatcgttt tgccaatcat attcgatcca ctcattgcca
     2581 ggtcgttctc cgctaatccc gtatgccgac agataacagt ttttcatcgt caagaaatat
     2641 ggcttatcaa agatgcttct gcaaagtttc acagaatact caacagcttc gcttcccgaa
     2701 cagagaaaaa cgcacttgcc atccactatt ccggtgatat caaaaatttg gctcgccgcc
     2761 tcatccataa ccggattaag atagcagtag cccgaatgaa tcatctttgc cgactgttcc
     2821 gatataactc gcgtaattcg tggatgacaa tgcccaaggg gcgtacacca gatacccgac
     2881 tccagatcaa ggtaactatt acctgagtcg tcaaaaagag tgcagttttg cgccctaata
     2941 atattatcat actttagatc atgtccggtg ctgttgatta ttctcatttt attcctttat
     3001 atttagacaa gatacttctt tgcaagtatc tcggcatctt taatgccctt gtccaactga
     3061 taaacagatt ttgccttgtt actgcaatca atgagccctt attttttcat ccataatttt
     3121 ttgtgccttt gcaaaaatca tgggttgcag ttttctttga atacaaattt catgcaactc
     3181 tttatcaaag gttgcctcca ttgacaccca cttgccattt aaaaaaacct caggaaatgt
     3241 atgaggaaat gggtctaccc agtttttata tgcaaagcca ggtaaaaaat catctaaagc
     3301 tgttttgtga acatacataa aatgaaaacg tacttttttt gactttctct ttacattttt
     3361 cttatattat ctttaccagt atttactatt tattaacctg taccataacc gcgaggtatt
     3421 ggtaacatgt gcttgattaa agcgtctcat aacgtaaata tggacgtcgg aggacggatg
     3481 agcggaactt ttcaccagca acttaagacc gaaatggaca aaatttttga atccgaaggt
     3541 cataaatacc ctcaagatga gatattacgt cttgatatgc actgccacga taaaaacagc
     3601 gaccagcctg atgaactaat tgggcgtata ctgaacattc cggagacatg gcttcctcca
     3661 aaatcattga ttaaaattct caaaaaaaac ggatcaactg cctttacagt aacaaatcat
     3721 aacaacgccc gtagctgcta ccagctgcaa gacgcaggtt acgatgtatt aaccggtgcc
     3781 gaatttacag ttacagtacc tgattttaac agtcgaatcc atgttttggc ttatgggttt
     3841 acacccgaag aagaaactgt gctcaatcgc aaacgctcaa atctttacaa atttcaggaa
     3901 tatgctttaa gccacgacat acccacagtc tgggcacacc cgctatttca ttataaaaaa
     3961 aacagcatac ccgacatggg gctttttgag aagttatcac ttgtttttca aagatttgaa
     4021 actgttaacg gtcaaaggga cacttggcaa aacctgcttg taaatcagtg gcttgacaca
     4081 ctcacaccag aaataattga ccaattcgca aaaaaacaca aaatgcctgt taacgctttt
     4141 caggaagatc cctacacaaa aatacgtaca gcaggctcag attcgcatat ggggcttttt
     4201 gctggacaaa ctggaacaat tcttcatata cctgggctgc aaaagcgact tgaaactgaa
     4261 accgcatcaa agcttgctct agaagctata cgcgaaggtc gcacagcact tttcggaagc
     4321 acccatgaca gcgaaaagat gacagttgcc tttatagact acttctgcca aatagggctc
     4381 aaccttgaag atccaggact tttaagaata cttttccata agggtgaaac aaaagataag
     4441 ttgctggcca tgtttatgac taacggtttt cttgaactgc agcgccaccg attaacaacc
     4501 ggttttatta aaacttttca caaatgcttt ttaggcgcac cgcctcgatc tcttgcaaag
     4561 cttattattc ctaaagacta tagccccgtt tttgatcttg caacaagcct tgccgatgta
     4621 aaaaaaatgc ctcacgcaga gcagccactt ttgtacaaag aaaaaatcga aaacatttat
     4681 agccatctta cagatcttgt tattaaacgt ctcgagggca agctcaaaaa catgccacaa
     4741 ataaaagatg aagatatgag caaaacaatt gttgaactcg atattccttc tcaactgcga
     4801 ctgctttttg aaaaggacaa ttcactacgt agcaaattta cttttttgcg aaagaaacca
     4861 gataaatctg atccatttaa attagacagg tttcttgacg gattatcgtt tccaatgctt
     4921 gcatcaacag ttataaccgc tgcgaccttt gccagtgcac gtgttcttta caacacccgt
     4981 cctcttctta atgcatttgc tgaggcgaca gggaaaactc ctcatcccaa aagaatgcta
     5041 tggttaaccg ataccttcga agatcgcaac ggtattgcaa ttgtattaaa gtctattttg
     5101 gaagagatcc gacgtcgaga cctaccgatt gatatattaa cctgtagctc caccgtaaaa
     5161 ccaggtaaaa acctgatagt cgtaccacca aaagccgaat ttgttttacc cttctatcaa
     5221 catcaacccc tacgtatacc cgatatatta caactacatc agatagtcaa agataacgaa
     5281 tacgaccgcc ttatgtgctc aaccgaaggc tttatgggag cagcgactct ctttttaaaa
     5341 aatgcctatt cgattccagc cttcttttat gtgcacacag actggaccac ctttggacaa
     5401 aaagtgctaa acctgagctc ctcggcacaa agccgtgttc gacgtttact cagagcggtt
     5461 tatcagcagt ttgataaaat ctttgtgctt aacagtgatc aacaaaaatg gcttgtgtca
     5521 aaagccatgg gcatacccga taaaaacgtt catttaaccg cccactggac agacaaggag
     5581 tacaggcccc aaagaaaatc caagaaacag cttttcggtg tttcagagga tacacctgtt
     5641 atgcttttta ccgggcgaat aagccacgaa aagggtgcaa ctgaacttcc tgcaatctat
     5701 aaacggataa ataaggtcat tccgggagtc aaactcgttc tggctggtac cggtcccgaa
     5761 gaggaaaaac tgaaaaaaat attacctgat gctctttttc taggctgggt tgaccacacc
     5821 gaacttccga aaatttactc atccgcggat cttatgattc tgccctctat ttttgatact
     5881 tttggatgcg tgattcttga agctctaagc tgcggaactc cggttatatc atacgcaaat
     5941 aaggggccaa aggatattat aattcatgag aaatgcggtt ttctggcctc aaacaggaac
     6001 aagatggccg actacgcagc ctcttatcta tcgtcaccaa agagatgggc atcttttaga
     6061 tcagccgcat taaaaagagc agctgactac aatccgacag atatttttga ttcgttactg
     6121 gaggatgtcg gtctttcaga acagaagttt aaagcaaaaa acagaaccac agcctaaaat
     6181 atttgcgtta tcttgtaatc cttttggcgg ccttggcgcc cgagctccat gcggctgtta
     6241 tgccgcgact cgttcccgcg ccatctccta ccataaacat gttctctttt acttcgaaaa
     6301 accggttaat aaaaaccgga cggtttgcgt aggtttttat ttcggggtaa taaagaattg
     6361 ttgaaggatg caaaactccg ggaattattg tatcaagcat ttttaaggag ctccagatat
     6421 gccgtaatat tttggctggc acagccaagg ccaggtcgcc tggtgtggct gacgcaagcg
     6481 taggcttaaa atcataaaga tcgccattaa aggttttact gttagaacgc ttgcccaatc
     6541 taaaatcgcc gaccctctgc atcatgggct gaccaccggc aagctgcata gccatctgtc
     6601 ctaatacctg tgcgaaatac tgtccacttg ccacagggtc agtaagttcg atagtcttta
     6661 gcatcgcaaa atttaccagg ttattcggtc tgtttttctg agacagtgag tgaccgttta
     6721 ccgaatagta gtgctcatat ttctctttta caacatgtgc cgctcctgaa tttgtgcaaa
     6781 aactgcggat tttataagga aagacaaatt tggggtcata ataatcctta actatgggat
     6841 agttctcgct actacactct accctgattc cgatatctac cgaattatcg ctaaaaccaa
     6901 tcccgttttt tttcataaca tcttgaagcc agtcaaaacc agcgcggccg ggtgcagcta
     6961 ttacatattt aaagcctacc tttttaccat ttttcagata aaccaagcgc tcttcataat
     7021 ttatcgcaag cgcatcagta tcacgccata cggtaatgcc accctcttca agttgctgta
     7081 taagagagcc tatgagagcc ggagccttat ctgtccccac atgcgcctgg cgtatatcaa
     7141 gcagatcgac accgagggca gccgcccttt tcttatatac ttcaagattc tttttttcag
     7201 ttatacgcgg attcagatat ttttcaacag taaccagttc attctcggcc tcatcctttg
     7261 tccaaagcag ctctggaaaa cccaccggat atgtatagtt ctgtttacag tcattacgca
     7321 atcctccact tgaaattgat cttttttcaa tcatgagtac cgaactgcat cccttttcca
     7381 gcagaccaaa agccgcgcca agcccagcag gtcctgaccc tatgataaca acttcaaaat
     7441 tgttcacagg ttctctccaa tcatacataa aattacaata ccagtattat caatttaaca
     7501 tatattgaca aaataaccca agaaaaactt gactattaat acctttagcc gtccaaataa
     7561 acaatgcact ttttcaaaac agaaaaaaaa aagatcggcg caactaaaac cgccacaaga
     7621 aacaatggag accaaataat tagtaaaccg gctcccataa cgagacgcct cacgattata
     7681 gctctttatc tgtctgtttg gacactgcta aacggttctg taacatcatt tacaatcaga
     7741 gaatatacat ttaataaaaa aaactatgtt tcgctttacg atattgcaac aaaaaccggc
     7801 ataaatcaaa cctttgacgt tatttcacaa agaggtaaac tgtaccacaa acatcattca
     7861 gctgtttttc aagcgggtta cgcagcgtac ctgattgacg gaagggttta ccttagcgat
     7921 tatcccgtta ttcgaaaaaa cggagagata tttatacctt cacttttttt ccgtgagatg
     7981 tgctctgcct tttttccaga attagaggta aggctggcca ataatcacta ctctctaaag
     8041 acaaaaacga aaactgaaga tccggtacca tttttaacac cagattcgcc accaaaagaa
     8101 aaaatcgcct ttatctttat cgatccggga cacggaggac gtgatcctgg agcaataggc
     8161 ggcaagattc gagaaaagga tcttaccctt aaactttcaa gaaaaatcag ggattatcta
     8221 aaaaagaatc tgaagggtgt acaaattctt atgacccgtg acaaggatca ttttatcgag
     8281 ctgggcaaac gcaccgaaat ggcaaatcgc aagttgaaaa agggtacaaa tggtctcttt
     8341 gtcagcgttc acgttaatgc gtccatatca tcacgcagca acggtcatga gacctattac
     8401 ctttctcaaa atccgacaaa tgaagaatcc cgtgctaccg cgtctcttga gaataatgtt
     8461 gttgtgctcg aatcaaagaa gcacaagcct tatcaggatg tggaatacgt tgaagccctg
     8521 atgttgacaa cccagataca aaaagagagc gccctgttgg cagacaatat tcaagacaga
     8581 atgaaaaaga atcttcgcga aacacgttct cgtggagtaa aaaaagccga tttttttgtt
     8641 ttaagaggtg ccctaatgcc agctgtactt gttgaagcag gatacataac aaacagcaaa
     8701 gaacgaaaac tgcttacaca gaaggatttt caggatcgca tcgcaaaaag tgttggtgat
     8761 ggtgtgatag attttattaa agagtataat aaataggggt ttaagcaaaa atatctaggt
     8821 ttcgtcccag ggccggtccc gatgaaacaa ctccacgtaa accataaagg tgatttgacc
     8881 tgaaatattc attcattcga ttaagcgctc ctcttcctat agcgccctcg ggctgaaccg
     8941 gcgttcgccg aggaccaaca cgcttaacag ccgaaactgg atttttggta tcataagctg
     9001 cttttatata gatcctacga ttattttgcg aaggcgaaag ttgtacaaca gaaacagccg
     9061 acgagatatt cattttacgt tccccgtaaa gattagtaca aattaataac aatcaacaat
     9121 taatgatcgg caaacagcaa tcttcacttt aaagacaatc attcaggatt aaaaaaagtg
     9181 taaccgttat catacaacag ccgaaatacc tcgtcctgag taggaatccc tccttgacca
     9241 cctaccgaac
//
LOCUS       DAOTGZ010000101         9096 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_3064
            NODE_9119_length_9096_cov_5.016923, whole genome shotgun sequence.
ACCESSION   DAOTGZ010000101 DAOTGZ010000000
VERSION     DAOTGZ010000101.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868088
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 9096)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9096)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:22:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,785
            CDSs (total)                      :: 2,751
            Genes (coding)                    :: 2,732
            CDSs (with protein)               :: 2,732
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 12 of 19
            Pseudo Genes (internal stop)      :: 5 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9096
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9119_length_9096_cov_5.016923"
                     /isolate="AMR_MDS_3064"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..739
                     /locus_tag="P5123_07835"
     CDS             <1..739
                     /locus_tag="P5123_07835"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRX16208.1"
                     /translation="HIMTGRYTEAEQDIQNALQIRESINLKKMLSAVYIKQNRFEEAS
                     IILADLDKRSPSNADIKYQLGSLYYKASDDKWVKTFGALFNLTNKNKTLSKKYSKAYL
                     LLANHYFSQKEYNRSTHIYQAITAEMLEFKDKNQAAHSFFQTKNYKEAIELFESISLA
                     QPEKLLLAKAYLKTGHESKARDSIEYIVLHTSAKQAIVEDPDLGPLAKKIIQEDMDAK
                     EELRKAEEAAAKKRELEEKVKTDETKK"
     gene            833..1603
                     /locus_tag="P5123_07840"
     CDS             833..1603
                     /locus_tag="P5123_07840"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRX16209.1"
                     /translation="MSLLLIPEIILYLSGFYFLTFTKTLSFEDLTALSFSILLRIVIM
                     TRLHQTKYIYNSLVSGGTLLATVIAFIFFNFGIPFALTLSPILFAIFLFLQFQKDFNI
                     CNYIIKGLLFSFFCLALLCLLTKSGNPFKALTPFMIFINTNFYHPVFLIIIITMFFII
                     VSNLSPRSILVFHFFADAALLLITGIIVVDTAVSIHNRHSIILQIRIILITTILTVYA
                     LSFSQLGIIIPLAVSYLFFIYSMNTGGTIYNENRISLL"
     gene            1578..2339
                     /locus_tag="P5123_07845"
     CDS             1578..2339
                     /locus_tag="P5123_07845"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HRX16210.1"
                     /translation="MKIESLCYKIKKKTILDDISFDFKEGMITGLIGRSGSGKTALLN
                     VLAGAVEHTDGRILQNNEILSAPKRKKISMLFPGKTDYSPYMTIRELILSGTPRHFYK
                     KREAYSQIEDFIHAVCLYFHLTPYLQFHCGEVPDAILKKSYLAASFITLKPLMFLDNP
                     DTHLDPLSLIQLINLIHHICIEKRVRFVIASHNINFIFSCCTHYIALGKGQILFSDKK
                     EKITDQIMGEIFGMDIICNHNIANGSPEFHFYHQV"
     gene            complement(2330..2674)
                     /locus_tag="P5123_07850"
     CDS             complement(2330..2674)
                     /locus_tag="P5123_07850"
                     /inference="COORDINATES: protein motif:HMM:NF013868.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="STAS domain-containing protein"
                     /protein_id="HRX16211.1"
                     /translation="MEFSYVVEDYEYFKLIKLSGNLSVISAEEFERAVNKITATHSVI
                     IDCANMEILTSAGVDSFVNVSLNARDTGHRVMILHLDPDYYHLIETLNILDYLIIVET
                     LDEGRVKIEYYT"
     gene            complement(2727..3182)
                     /locus_tag="P5123_07855"
     CDS             complement(2727..3182)
                     /locus_tag="P5123_07855"
                     /EC_number="3.6.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012514.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HRX16212.1"
                     /translation="MSLLNNKRIRIRVAGILIENGNVLMVCHQKNGKSYWLLPGGGVD
                     YGETLPQALRREFEEEIGIKVEVHDLLFSIDSISPSGKRHIVNLVFICKYSSGVYRLG
                     LDKRLSDFKFLSIEELSNATVYPSLKKQLIDYVAGEIGQKIYLGSHWNK"
     gene            complement(3179..3901)
                     /gene="rnc"
                     /locus_tag="P5123_07860"
     CDS             complement(3179..3901)
                     /gene="rnc"
                     /locus_tag="P5123_07860"
                     /EC_number="3.1.26.3"
                     /inference="COORDINATES: protein motif:HMM:TIGR02191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004525 - ribonuclease III activity
                     [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease III"
                     /protein_id="HRX16213.1"
                     /translation="MHDFKNRSKDLNRLQKILKVKFTNLDLLNRSLTHRSYANELPYP
                     VKENERLEYLGDSVLGLVINEYLYKRFESYHEGDLAKIKSTVVSEEMLAAVAFELKIG
                     QFILLGKGEENSGGRSRHSILANTVEAIIGAMYLDHGLKASKKFILSVFKKYIDQVDK
                     IPTMRDPKTSLQEIVQKKYKKKPDYILLKEEGPDHEKLFTVALTINGRVVIEGSGTSK
                     RRAEVDAARKILNKIKNERFEV"
     gene            complement(3976..4170)
                     /gene="rpmF"
                     /locus_tag="P5123_07865"
     CDS             complement(3976..4170)
                     /gene="rpmF"
                     /locus_tag="P5123_07865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002831400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L32"
                     /protein_id="HRX16214.1"
                     /translation="MAVPKRRKSKSKVRMRRANVKIASPNLRPCPKCGAFVLSHKVCP
                     ECNTYKGRVIIEKKAPVVEA"
     gene            complement(4276..4965)
                     /locus_tag="P5123_07870"
     CDS             complement(4276..4965)
                     /locus_tag="P5123_07870"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HRX16215.1"
                     /translation="MSKVNVEQNKFEKIAFSVFGYISSHRKIFIASFSLIIVVVLAII
                     AFAVYYDKHQSEIVVQYEAILDEYSRLIEEEKKEEAVNYAVSEIVKLSDSAKWGYIKD
                     NGYYLAAGILYHNKRYEEALEYYNKFVDNSGSSELIPLARIQIAVTLEWLERNDEAFE
                     IYKNLEVDLADTKFIEQIIYDLGRHYERTGDVKKAKEYYSRLVSEYIGSFYEKQAKKR
                     LMLMSYKPDAS"
     gene            complement(4958..6643)
                     /locus_tag="P5123_07875"
     CDS             complement(4958..6643)
                     /locus_tag="P5123_07875"
                     /inference="COORDINATES: protein motif:HMM:NF012784.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S1 RNA-binding domain-containing protein"
                     /protein_id="HRX16216.1"
                     /translation="MNDKIDPDFEDSISMDELLAESELAFEGGVLKGEVVSVDNEFAF
                     VNTGGKTEGRVLLSEFDVKPKPGEVVDVVVVNRRGSDGMSKLSHRQAKQAAEWECFEK
                     SGLSVGNDIKGKIVEARQNGAMVEFDGYRGFMPMSHAADIRVKDAVQNKTEFELRIIK
                     IDPRKKSIIVSRRALVDEKKDESWKELLASVSEGDVVEGTVARIVNFGAFIDIGGFEA
                     LLHNNDLSWKKVAYVKDFLKIGDKSKFKVLSINKSDRKISLGLKQLKEDPWLSVKNRF
                     SIGTVYNGTVTGVAHFGVFVELEEGVEGLVPASEIDWTKKNQGARNQPAKGDNVKVKI
                     LSIDSEEKTISLSIRQTIENPWKAIAKKYPVGTVVKGVIKNKMDFGLFIQVEEGIDAF
                     VHVSEISWDDKVKNPLEPYKEGDTVSCKILSVNVDEMKIAAGIKQLERSPWEAVKEKF
                     PPRSIVQGKITSVKSFGVFVKLDENVEGMIHISEVSKNKVDDLSELFKEGDTVEAVVL
                     DVDVAKKKISLSIKNIEMQAEKEELSKILNAQSSNTASIGDLIKLKGEETENE"
     gene            complement(6781..7458)
                     /gene="cmk"
                     /locus_tag="P5123_07880"
     CDS             complement(6781..7458)
                     /gene="cmk"
                     /locus_tag="P5123_07880"
                     /EC_number="2.7.4.25"
                     /inference="COORDINATES: protein motif:HMM:NF014300.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004127 - cytidylate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0015949 - nucleobase-containing small
                     molecule interconversion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(d)CMP kinase"
                     /protein_id="HRX16217.1"
                     /translation="MKKITVAVDGPAGSGKSSVSRQVAAMLGITYIDSGAIYRAITWF
                     FLEKYGDIDSETPFGNGIKGLDIRQEYYSDRTTKTFVNGLDVSQLIRSEEVVKSIQWV
                     SSNKAVRDFVTTLLREWAGEKSIIMDGRDIGSVVFPNADLKIYLDASVETRAKRRYDE
                     YVATGKNVDLKAIKKQIIQRDSQDTAREFGALKRCEDAVFVDTSDMTQDQVVNKLTEL
                     IENQDSV"
     gene            complement(7460..7984)
                     /gene="scpB"
                     /locus_tag="P5123_07885"
                     /pseudo
     CDS             complement(7460..7984)
                     /gene="scpB"
                     /locus_tag="P5123_07885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017215788.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="SMC-Scp complex subunit ScpB"
     gene            complement(8506..>9096)
                     /locus_tag="P5123_07890"
     CDS             complement(8506..>9096)
                     /locus_tag="P5123_07890"
                     /inference="COORDINATES: protein motif:HMM:NF014654.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="segregation/condensation protein A"
                     /protein_id="HRX16218.1"
                     /translation="SEFIAMASELLYYKSKILLPTGEIEDEFFIPPLPRELVAKLLEY
                     KRHQKSALEIKKLYDKQSESFTRENDFSKVKDDDEYTTMSLFDLLNAFVDVMSVSAPV
                     EEREIVFDEILVSDRIDFIVNLLRSKEQITFKQLFHSIPSVPEIVSTFLACLELAKMH
                     SIKVMQHKQYGTIHLFRNFDPDTYRINNDTMELTHD"
BASE COUNT         2877 a         1935 c         1343 g         2941 t
ORIGIN      
        1 ccatattatg actggcagat acacagaagc tgaacaggac attcaaaacg ctcttcaaat
       61 tcgcgagagc attaatctaa aaaagatgct ctctgccgtt tatataaagc agaaccgctt
      121 tgaagaagcc agtattatac ttgcagatct tgataaacga tcaccttcaa atgccgacat
      181 caagtatcaa cttggctcac tatactataa agcttctgat gataaatggg ttaaaacctt
      241 cggagcactt ttcaatttaa ctaacaaaaa caaaacgctt tccaaaaaat actccaaagc
      301 ttacctgcta ctagcaaatc actatttttc tcaaaaagag tataaccgct caacgcatat
      361 ttatcaagcc atcactgcgg agatgctcga atttaaagat aagaatcagg cagcccatag
      421 tttttttcag accaaaaatt acaaagaagc aatagaactt tttgaatcaa tatcgcttgc
      481 acaacctgag aaattgcttt tagcaaaggc ctatttgaaa accggccacg aatcaaaggc
      541 tcgtgattca attgaatata ttgttttgca tacctctgca aaacaagcca ttgtcgaaga
      601 ccctgaccta ggaccactgg caaaaaaaat aatccaggaa gatatggatg caaaagaaga
      661 actcagaaaa gcagaagaag ctgcggcaaa aaagcgtgaa cttgaagaaa aagtcaaaac
      721 cgatgaaaca aaaaaataga gtctatcgct agttactatc tctattttat ttccgaaaat
      781 aagtaattat tccaagctcc cttattaaat aaatataagg atagattgca ctatgagcct
      841 tttactgatt cctgaaatta ttctctactt atctggtttt tattttttga cttttacaaa
      901 gacgctctct tttgaagatt taacagcctt gagcttttcg atacttttac gtattgtaat
      961 catgacgaga ctacaccaaa caaaatatat ttataactca ttagttagtg gtggaacatt
     1021 acttgcaacc gtgatagctt ttattttttt caatttcggc attccatttg ctttaacact
     1081 ttcacctatt ctttttgcaa tattcctctt tttacaattt caaaaagatt tcaatatttg
     1141 taactatata atcaaaggat tactcttctc atttttttgt cttgctctgc tctgtctttt
     1201 aacaaaatcc ggcaatccct ttaaagccct tacaccattc atgattttta ttaatacaaa
     1261 tttttatcat cctgtttttt taataattat tataactatg ttttttataa ttgtatctaa
     1321 tttatcccct agatcaatac ttgtttttca ttttttcgct gacgcggcac tactactcat
     1381 tacaggtatt attgttgttg atacagctgt ttcgattcat aatcgacatt caattatttt
     1441 gcaaattaga attattttga ttacaacaat tttaacagtt tatgccttat cattcagtca
     1501 attagggata ataattcctc tagctgtctc ttatctgttt tttatctatt ccatgaatac
     1561 cggaggaacc atttacaatg aaaatagaat ctctttgcta taaaattaaa aaaaagacta
     1621 tattggacga tatatcattc gattttaagg aaggcatgat aacgggactt attgggagat
     1681 cgggatcagg caaaacagct ttgttaaacg ttcttgccgg agcagtggaa cacactgatg
     1741 gaagaatatt gcaaaacaat gaaattttat cggcacctaa aagaaaaaaa atatcaatgc
     1801 ttttccccgg taaaactgat tactcaccat atatgaccat cagggaactt atcctttctg
     1861 gaaccccaag acatttttac aagaagagag aagcctactc acaaattgaa gattttatcc
     1921 atgcagtctg tttatacttt catttaacac cgtacttgca atttcattgc ggtgaagttc
     1981 cagatgcaat attaaaaaaa tcatatcttg cggcatcttt tattaccctg aaaccgctaa
     2041 tgtttcttga taatcctgat acccaccttg accctttatc acttatacag cttattaatc
     2101 tgattcatca tatttgtatt gaaaaacggg taagatttgt tattgcttca cataatataa
     2161 attttatatt ttcgtgctgc acacactaca ttgctcttgg taaaggtcag attctattct
     2221 cggataaaaa agagaaaatc acagatcaaa taatgggaga aatatttgga atggatatta
     2281 tttgtaacca taacatagca aatggttccc ctgagtttca tttttatcat caggtataat
     2341 actctatctt cacacgccct tcgtcaagag tttctacaat aatcagataa tccaaaatat
     2401 ttaaggtttc tattaaatgg taataatcag gatcaaggtg aagaatcatt actcggtgac
     2461 cggtatctct ggcattaaga cttacattta caaaggaatc aacacctgca gatgttaata
     2521 tttccatatt tgcgcagtcg ataataacac tgtgagttgc agttattttg tttacagctc
     2581 tttcaaactc ctcagcactg ataaccgata gattaccgct taatttaatc agtttgaaat
     2641 attcgtaatc ttcaacaaca tatgaaaatt ccatctaaac ccacttataa aatattatgg
     2701 taatctttaa aattaattat actgctctat ttattccagt gtgaaccaag atagatcttc
     2761 tgcccgattt ctccggcgac ataatcgata agctgttttt tcaaagacgg ataaactgta
     2821 gcgtttgaga gttcttcaat agaaagaaat ttaaaatcgg aaagacgctt atccaaaccc
     2881 aaacggtaaa ccccgctgga atacttacaa ataaacacaa gattaacaat atgtctcttc
     2941 cccgaaggcg aaatagaatc aatactaaaa agaagatcat gtacttctac cttgatacct
     3001 atctcttctt caaactctcg tcgtaaagcc tgtggaagtg tctctccata atcgacgccc
     3061 ccacccggca aaagccagta actttttcca tttttttggt ggcataccat taaaacgtta
     3121 ccgttttcaa taagaatacc agcaacacgg atacggattc ttttattgtt aagcagactc
     3181 acacttcaaa tctctcgttt ttaatcttat ttaaaatttt acgggccgcg tcaacctctg
     3241 ccctacgttt gctggtaccc gaaccttcaa taacaacacg cccatttatc gttaaagcaa
     3301 cagtaaacag cttctcatga tctggcccct cttcttttaa taaaatataa tccggctttt
     3361 ttttatattt tttttgtaca atttcttgaa gagaagtttt aggatccctc atggtaggta
     3421 ttttatcaac ttggtcaata tattttttaa aaacagacaa tataaacttt ttactggcct
     3481 taagaccgtg gtcaagatac atggctccga ttatcgcttc aacagtattg gcaagaatag
     3541 aatgacgact ccttccacca ctattctctt cgccctttcc aagcagaata aactgaccta
     3601 ttttcaactc aaatgctaca gcggccaaca tctcttcgga aacaactgtc gacttgatct
     3661 ttgccaagtc tccctcatga taagactcga aacgtttgta aagatattca ttaattacaa
     3721 gccctaaaac cgaatcgcca agatattcca gacgttcatt ttctttaaca gggtagggaa
     3781 gttcatttgc gtatgaacga tgagtaaggg aacggttaag aagatccaga ttagtaaact
     3841 tgaccttcag tattttttgg aggcggttta aatcttttga gcgattttta aaatcatgca
     3901 taaaaaatcc tgaaaaaata aaagggtgtc aaaggacacc cggttatttt aatacctaac
     3961 gcattagtca aagaactagg cttctacaac aggcgctttt ttttctataa taacacgtcc
     4021 tttataagta ttacactcag gacaaacctt atgagataaa acaaacgcgc cacactttgg
     4081 acaaggacgc aagtttggag acgcaatctt tacatttgca cgacgcattc tcacttttga
     4141 ttttgatttt cttctctttg gaacagccac tttatatctc cataaataac attctcattt
     4201 tgcattgagg aacaaaatat ttttaacacc caatttgtca agcacataaa accagcatat
     4261 cagcctaaac tgctttcaag aagcatcagg cttataactc ataagcataa gccgtttttt
     4321 agcctgtttt tcgtagaaag agcctatata ttcagaaaca agacggctat aatactcctt
     4381 agccttcttg acgtcacctg tacgctcata atgacgacct agatcatata ttatctgttc
     4441 aatgaatttg gtatcagcca aatcaacttc cagattcttg tatatctcaa atgcctcatc
     4501 gttgcgctct agccactcaa gcgttacagc aatctgtatt cgcgctaatg gaattaattc
     4561 cgaagatccg ctattatcta caaatttatt ataatactca agggcttctt cgtagcgttt
     4621 gttatgatag agtataccgg cagcaagata ataaccatta tctttaatat aaccccattt
     4681 cgcactatca gaaagcttta caatttcgct aacagcatag tttacagctt cctctttttt
     4741 ttcttcttct atcaaacgac tatactcatc aagaattgct tcgtattgaa ctacaatttc
     4801 actctggtgc ttgtcataat aaacagcaaa agcaataatt gccaaaacca caacaataat
     4861 tagagaaaaa gaggctataa atatttttct gtgcgatgat atatagccaa agacactgaa
     4921 agctattttt tcaaatttat tctgctctac attaacttta ctcattttct gtttcttcac
     4981 cttttaattt tatcagatcg ccaatagacg cagtattaga actttgagca tttaagatct
     5041 tactcagctc ctctttttcc gcctgcattt caatattttt aatactaagg gaaattttct
     5101 ttttagcaac atcaacatct agaacaactg cctctactgt atctccctct ttaaaaagct
     5161 ctgacagatc atcaacttta ttttttgaaa cttcagatat atgaatcata ccttcaacat
     5221 tttcgtcaag cttaacaaaa acaccaaaag acttaacaga agttatcttt ccctgaacaa
     5281 tcgaacgagg aggaaacttc tcttttacag cctcccatgg cgatcgctct agttgcttta
     5341 tacccgctgc gattttcatt tcatcaacgt taacagatag aattttacag ctaacagtat
     5401 cgccttcctt gtacggctca agcggatttt taactttatc atcccagctt atttccgata
     5461 catgcacaaa ggcatctata ccttcttcca cttggataaa aagaccaaaa tccattttat
     5521 tttttattac accctttacg acagtaccaa ccggatattt tttggcaatt gctttccatg
     5581 gattttcaat ggtctgacgt atactaagag atattgtttt ttcttcactg tcaatactta
     5641 gaatcttaac tttaacatta tctcccttgg ctggctgatt ccgggctccc tggttttttt
     5701 tagtccaatc gatttcagaa gctggaacaa gcccttctac accctcttca agttcaacaa
     5761 aaactccaaa atgtgcaaca cccgttacag tgccattgta aacagtacct atagaaaatc
     5821 tattttttac tgaaagccaa ggatcttcct tgagttgttt aagacccaaa gatatttttc
     5881 ggtcactttt attaatagaa agaactttaa atttagattt atccccaatt tttaaaaaat
     5941 ccttcacgta tgcaactttt ttccatgata aatcattatt atgcaacaat gcttcaaagc
     6001 cacctatatc aataaaggcc ccaaagttaa cgatacgagc aacagttcct tcgacaacat
     6061 ctccctcgga aaccgaagca agaagttctt tccaactttc atctttcttt tcgtcaacga
     6121 gcgctcgtct tgagacaatt atgctttttt ttctagggtc aatttttatt attctaagtt
     6181 caaattcagt tttgttttgt accgcatcct tcactcgtat atctgcagca tggctcatcg
     6241 gcataaatcc acgatagccg tcaaactcga ccattgcacc attttgtctg gcttcaacaa
     6301 tctttccttt tatatcgtta ccaacagata aaccgctttt ttcaaagcat tcccattcgg
     6361 ccgcctgttt tgcctgtctg tgtgaaagtt tagacatacc gtcactacct cggcggttaa
     6421 cgacgaccac atcaacaact tcacccggtt taggcttaac atcaaactcc gataaaagaa
     6481 cccttccctc agtttttcca ccagtgttaa caaacgcaaa ttcattatca acagaaacaa
     6541 cttctccctt aagaactcca ccttcaaagg ctaattcgct ttctgcaagc aactcatcca
     6601 tagagatact gtcttcaaaa tccggatcaa tcttatcgtt catatgctct actttcccac
     6661 attcgaatac ttttaaaaac tactctaacc ggacgccagc tattttacct gtaaaccgct
     6721 ttttaaacgt cacaatcgat attaatttta aaagcacccg ttattataaa ttttattatt
     6781 tcagacacta tcctgatttt caatcagttc agtcagctta tttacaacct gatcctgtgt
     6841 catatcggaa gtatcaacaa ataccgcatc ttcgcatctt ttaagagctc caaattcacg
     6901 ggcggtatcc tgagaatcac gctgtataat ctgttttttt atggctttta agtcaacatt
     6961 ttttcctgtg gcaacatatt catcatagcg ccttttggct cgagtctcga cagaggcatc
     7021 aagatatatt tttaaatcag catttgggaa aacaaccgaa ccgatatccc ttccatccat
     7081 aatgatcgac ttctcccccg cccactcacg caaaagagtg gtaacaaaat ccctaacagc
     7141 tttgtttgac gatacccact gaatactttt aacaacttct tccgaacgga tcaattgaga
     7201 tacatcaagc ccattaacaa atgttttggt agttcgatcc gaataatatt cctgtcgaat
     7261 atccaatcct tttataccat tgccaaaagg agtctcagaa tcgatatctc cgtatttctc
     7321 tagaaaaaac catgttatgg ctcggtaaat tgcacctgaa tcgatatagg taataccaag
     7381 cattgccgct acctgacgag aaacgctact ttttcccgat ccagctggac catcaactgc
     7441 aactgtaatt tttttcactc tattctccgg aaaagtcagt ttcgtaagaa aattccttaa
     7501 tttctgacaa ttttggaagg tccgacaatt tgttcaagcc aaaaacccgt aaaaactcat
     7561 cagtcgtccc ataagctaat ggcctaccag gaacctcttt tctgccaaca ggcttgatca
     7621 agtttcgttt catgaggttt gccaccatca ttcgagaaga tacacctcta agttcttcaa
     7681 cctcagcaag aattacaggc tgtttatatg caattattga gagggtttca agcataccac
     7741 gtgttaatgg ttcttttcgc ttaaaaccaa gaattcgacg tatctgacgg gcataattag
     7801 ggttcgtaac aaactgataa ccatttgata tttctacaag ttttatacca ccgtcccgct
     7861 cttcatactc atcgatcagc gaatcaagga caatttttac atcagtttta tcgattttga
     7921 aatttttggt aaaaaaattc agagatagag gctcattaga tagaaacaaa agtgcctcaa
     7981 aaactgcatg ataattatct tcagatgtat cgtcagatgt atcgtcagat gtatcgtcag
     8041 atgtatcagc agatgtatca gcagatgtat cgacagatgt atcgacagat gtatcagcag
     8101 atgtatcgac agatgtatcg acagatgtat cggcagatgt atcggcagat gtatcggcag
     8161 atgtatcggc agatgtatcg ataccgatct cttctacacc atcgccatta tgttctgttg
     8221 cagactgatc cacaacagat tcggcactat ttacatcatt ctcttcattg ttaacaacac
     8281 ttttcttgtt ttcatccata gtattcactt aattgcaagg gagtttcgga ataattaccg
     8341 ttttaagtcg aagtcaaaaa acaacataat aatcactgac cacattatag atctatcgga
     8401 ctgcgactcc cgtatcctta aaccatgcca ctatatttga tattaattac aaattaattt
     8461 tttttgcgga tattaaaata ttgtaatctg gtctctatat tggctttaat cgtgtgtaag
     8521 ctccattgtg tcgttgttta tgcgataggt atcgggatca aagtttctaa acagatgaat
     8581 tgttccatat tgtttatgct gcataacttt aatactatgc atcttggcaa gctcaagaca
     8641 ggctaaaaaa gtagaaacaa tctccggcac ggaaggtata gaatgaaaaa gctgtttaaa
     8701 tgttatctgc tcttttgaac gcaaaaggtt cacaataaaa tcaattctgt cactaacaag
     8761 aatttcatcg aaaactattt cacgttcttc aacaggcgca gatacgctca ttacatctac
     8821 aaatgcatta agcagatcaa aaagcgacat tgtagtatat tcatcatcat cctttacttt
     8881 cgagaaatca ttctccctcg taaagctctc ggactgctta tcatataatt tctttatttc
     8941 aagagcgctt ttctgatgac gtttatattc aagcaattta gctacaagtt cacgcggaag
     9001 tggcggaata aaaaattcgt cttctatctc acctgttggc aatagaatct tagacttgta
     9061 atacaaaagt tccgaagcca tagctataaa ctcaga
//
LOCUS       DAOTGZ010000102         9003 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_3064
            NODE_9242_length_9003_cov_5.468149, whole genome shotgun sequence.
ACCESSION   DAOTGZ010000102 DAOTGZ010000000
VERSION     DAOTGZ010000102.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868088
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 9003)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9003)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:22:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,785
            CDSs (total)                      :: 2,751
            Genes (coding)                    :: 2,732
            CDSs (with protein)               :: 2,732
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 12 of 19
            Pseudo Genes (internal stop)      :: 5 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9003
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9242_length_9003_cov_5.468149"
                     /isolate="AMR_MDS_3064"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(31..1884)
                     /locus_tag="P5123_07895"
     CDS             complement(31..1884)
                     /locus_tag="P5123_07895"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRX16219.1"
                     /translation="MRRINLLLTAIILSAAMLFNVSCGDGSGSTSPKDGEPESSPNSY
                     ADEDPVKEPAKGSDVTTSTPSDTTSDPIITEEIVVEEIQTIAETEALPSNEEKAESDQ
                     SITDDSDNKSEQIASDITEDTDSSIDETPAETEITSGSDPVAESDSENTESTEPAVAV
                     NDSDDTTNSEADETDETDIPEIPAVAETPAPAPSADEILAEELPLIAADDDEPVTDDT
                     SITDEAGIVLEDAQVVAEPSEGSEWFTVPDQPLDNPEYAALAEELEQLVERAEQIIKL
                     IDKMPNDKARQAQIKRLEDIEQAIASIRNQIGYSDVQSGIYTASANQQLDLTVSGITE
                     PGYYSLSVVAKNVFGTLPDSYGYFNLSVSDNSGSAEGNILVPASDNTYQKGSALVYLN
                     PGTNNLKLKWTNDYYIEGSYDANIQIKNIMLNKTSASDRAQLAPYKDDLTRSALQYTS
                     SSDGVFWTRDGALIYQSGNSISFNYPMLDKGRYQIVIWARNHGEIKSKPQFKRFEFDV
                     YTSTGESELVSVNAHHKNFKAGKGSIDISGGDTTIEITWINDGPEDVILELKQIELKR
                     IGDYQGSALSAYLLGTDSGNRLLIIILLLFFTSIGIALSIYHKRQCIRVRK"
     gene            2392..3225
                     /locus_tag="P5123_07900"
     CDS             2392..3225
                     /locus_tag="P5123_07900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014453487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfide/dihydroorotate dehydrogenase-like
                     FAD/NAD-binding protein"
                     /protein_id="HRX16220.1"
                     /translation="MYKIIEKYSLAPQIEYARIKAPMISKNAEPGQFLIVVACEKSER
                     IPLTIADFDREEESVSIVFQKMGKGTHKIADFKAGDSFHAVLGPQGNPTEIEGEKHIV
                     MIGGGIGVAPVYPIARKFHENGVHVTSIIGYRGKDFLFWDDKLKAVSDEMIITTNDGS
                     VGHKGFVTDVLKQMIEDKKPIDRVIAIGPPIMMKAVADVTRPHGIATTVSLNSLMVCG
                     MGMCGACRVTVGGETKFTCMDGPEFDAHKVDFEELMQRLGMYKSEEKDMLDEYQGEDN
                     E"
     gene            3218..4618
                     /gene="gltA"
                     /locus_tag="P5123_07905"
     CDS             3218..4618
                     /gene="gltA"
                     /locus_tag="P5123_07905"
                     /EC_number="1.4.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013405902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004355 - glutamate synthase (NADPH)
                     activity [Evidence IEA];
                     GO_process: GO:0006537 - glutamate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent glutamate synthase"
                     /protein_id="HRX16221.1"
                     /translation="MSDRFKAIHMKEQPAEERKKNFLEVPCGYTEEEAVLEANRCLQC
                     KNAPCIEGCPVSIDIRSFIGHIQKREFDKAVNKIYEADILPCVTGRVCPQESQCQAKC
                     VLNNKGDAVSIGSLERYVADRDLERRREALKGEPVKISGKTKVAVIGSGPAGIACAAD
                     LGKYGYDVTIFEAFHKAGGVLVYGIPEFRLPKDIVDVEIRSLVQTGVKLVTNVLTGRS
                     FTIDELMAEGYKAVFVGSGAGLPQFMKLPGENLNNVYSANEFLTRVNLMKAHRYPEYD
                     TPVKYGKNCAVIGGGNVALDVARTALRLGSENVYLIYRRTQEEMPARLEEVRHALEEG
                     IQLLALTNPVAYHGDEAGFLQKIECVKMELGEPDESGRRRPVLVEGSNFFIEADVCVV
                     SIGSTPNPLIAMTSPLIETTKWGTIKVDEKGMTTMPGVFAGGDIVTGAATVITAMGAG
                     RTAAASMHEYLQNKKD"
     gene            4843..5031
                     /locus_tag="P5123_07910"
     CDS             4843..5031
                     /locus_tag="P5123_07910"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRX16222.1"
                     /translation="MKHADAIKTDKTDGNGKYVISITGVAFIAIMLYGKGLAVGEERT
                     HYDYSKVPDIDYMISDIA"
     gene            5195..6571
                     /gene="asnS"
                     /locus_tag="P5123_07915"
     CDS             5195..6571
                     /gene="asnS"
                     /locus_tag="P5123_07915"
                     /EC_number="6.1.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018047948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004816 - asparagine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006421 - asparaginyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine--tRNA ligase"
                     /protein_id="HRX16223.1"
                     /translation="MSVKRERIKHVLSIVEVGSRITVKGWVRTCRKVKDLIFIEINDG
                     SDIESLQVVAEPKIEEYKQLSELTTGSSLSVTGTLVESPAAGQRVELNAEEAEIYGLT
                     DASSYPLQKKRHSFEFLREIAHLRPRTNTFGAVFRVRNSLAHAVHSFFQQREFLYVNT
                     PIITANDCEGAGELFHVTTLDPLKQNKDYGDDFFGKKASLTVSGQLEGELFATALDRI
                     YTFGPTFRAENSNTSRHLAEFWMVEPEAAFNDINDNMDLAEDFLKHIILSVLDECPKD
                     MDFFDKRIKPGLIKNLEKIVSSKFERMTYTEALKTLIDSKEKFNYKVEWGCDLQSEHE
                     RFLVEKKIGSPIILTDYPRTLKPFYMYCNSDNKTVRGMDVLVPGVGEIIGGSEREYRL
                     DLLLDRMKELNLNEEDYDWYIDTRRFGTVPHSGFGLGFERAVQFVTGMENIRDVIPFA
                     RTPKNLKF"
     gene            6579..7028
                     /gene="rpiB"
                     /locus_tag="P5123_07920"
     CDS             6579..7028
                     /gene="rpiB"
                     /locus_tag="P5123_07920"
                     /EC_number="5.3.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013537968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004751 - ribose-5-phosphate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0009052 - pentose-phosphate shunt,
                     non-oxidative branch [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribose 5-phosphate isomerase B"
                     /protein_id="HRX16224.1"
                     /translation="MKIAIGSDHTGVDLKALIIKTFDDVDFMDFGPEAKESVDYPDYI
                     EKVAKAVQKGECERGIAICGTGIGASITANKVRGIRASLCFNGFMAEMTRKHNDSNVL
                     VLGARVTGEEVALYIVEKWLNTGYEGGRHQRRLDKVSAIENREIGGD"
     gene            7031..8683
                     /locus_tag="P5123_07925"
     CDS             7031..8683
                     /locus_tag="P5123_07925"
                     /inference="COORDINATES: protein motif:HMM:NF024777.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRX16225.1"
                     /translation="MRRFAIFTATIIVLFSMVTLYSSKRVTGIYDFTPERLHFHRVSP
                     IKTEKGFEYIISETTYERPDYLISDLTVSFNKKNYHSMDDSGKYRTSVSSYSWFKPEG
                     SAGDGAGYFYKRNHRLEFSNSSASWLGNNGDLGSFTIEFRLNPASFNDEAELFSRVGY
                     NSGTRNGILIQIKDRRIHCSFFDLFIDDKGQHYTYEFASAAKLQNKTWYYTALSYDRL
                     TGKVELFIDGKSQVMFYATDDGTSSGTLHTPSFFKNDMPNIVFGKNYFGYLDEFRLSY
                     RSFDDLKKLTALADKKHIDVGLTGRVPVNSEGVVTSPVYEFQHTGTMIKNFVWDQVLD
                     KETFIWFEIRLSDDKFDQYHQNPRWYRITNNQRNIYLMKTANLWLRGKYFQWRAHLIS
                     SPDGERSPVLQSIKMNYELDTPPIAPLFPECIEQGDGYVVLRWKKNVDHDFFGYKVYY
                     GVKSGKYEGVLVSDGSKPITNGSGDFVELKIDNRLVEENRLKDNKGLLSYPLLKNNVL
                     YYFAVTAYDSYRVNTSYNHESDFSKEIFGRPFGGSEIIVESK"
     gene            8701..8907
                     /locus_tag="P5123_07930"
     CDS             8701..8907
                     /locus_tag="P5123_07930"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRX16226.1"
                     /translation="MEENKLRNIISDLEIVNRNLQLVSQNKCLDDSESGLVREASGLT
                     KRAIQLLNEEEVRMEIYKLSDNDC"
BASE COUNT         2619 a         1583 c         2127 g         2674 t
ORIGIN      
        1 gggaatgctc cctgttgccg tggaagaaga ttatttacgt actcgtatgc attggcgctt
       61 atgatagatt gaaagagcaa ttccaataga ggtgaaaaat agaagtaata ttatgatgag
      121 cagacgattg ccggagtcgg ttcctaaaag gtaggccgaa agagccgaac cctgataatc
      181 gccgattctt ttaagttcta tctgtttgag ttctagtatt acatcttcgg gaccgtcatt
      241 gatccaggtt atctcgatag ttgtatctcc acccgaaata tctatgctgc ccttgccggc
      301 tttgaaattt ttgtgatgag cattaacaga gacaagctcg ctttcaccgg ttgaagtata
      361 aacatcaaat tcaaatctct tgaattgagg ttttgatttg atttcgccat gatttctggc
      421 ccagattacg atctggtatc gacctttatc gagcattgga tagttgaagc tgattgagtt
      481 gcctgattga tagataaggg ccccgtccct agtccagaat actccatcag atgaagaagt
      541 atactgtaaa gccgaacgtg taaggtcatc cttgtacggt gctagttgag cacggtcact
      601 tgctgaagtt ttgttgagca ttatgttttt tatctggata ttagcatcgt aagaaccctc
      661 gatataatag tcattggtcc attttagttt tagattattg gtgcctgggt taagatagac
      721 aagcgcagaa cctttctgat atgtattatc tgaagctggt accagtatat tgccttcggc
      781 agaaccgctg ttgtcagaaa cagagaggtt aaagtagccg tagctgtctg gcaatgtgcc
      841 aaaaacgttt tttgcaacaa cacttaggct gtagtagccc ggttcggtta ttcccgaaac
      901 ggtaaggtca agctgctgat tggcgcttgc tgtataaatt ccactttgga catcgctgta
      961 acctatctga tttcggatgc ttgcaattgc ctgctcgata tcttcgagtc gttttatctg
     1021 tgcttggcga gctttatcgt taggcatttt atcaatcagt ttgattatct gttcagctct
     1081 ctcaacaagc tgctcgagct cttctgcaag tgcagcgtat tcaggattgt caagcggttg
     1141 atcgggtacg gtaaaccatt cgctgccttc tgatggttcg gcaactacct gcgcgtcttc
     1201 caaaacgatt ccagcttcat cagtaattga tgtatcatca gtaacgggtt catcgtcatc
     1261 agctgcaatt agaggcaact cttcggcaag tatctcgtcg gctgaaggcg ctggagcagg
     1321 tgtttcggca actgcgggaa tttcaggaat atcagtctcg tcagtttcat ctgcttcaga
     1381 attggttgta tcatccgaat cattaacggc tacagctggc tccgttgact cggtattttc
     1441 cgaatcagac tctgctacag gatcggaacc agaggtaatt tctgtttcgg caggagtttc
     1501 gtcaattgac gaatcggtat cttcggtaat atccgacgca atttgttcgg atttattgtc
     1561 tgaatcatca gttatacttt gatcagattc agctttctct tcgtttgaag gtaaagcctc
     1621 ggtttcggct attgtctgta tctcctcgac tacaatttct tcagtaataa tagggtcaga
     1681 tgtggtatcg gatggagtcg atgttgttac atccgaacct ttcgccggct ctttaacagg
     1741 atcttcgtcc gcataagagt ttggtgaaga ttcaggttcg ccgtctttag gtgatgtgga
     1801 tccgctacca tcgccacagc tcacattaaa aagcattgcg gctgataata taattgcggt
     1861 tagaagtaaa tttattcttc tcattgtctc ctccttaaat cagagatgca aacttatggt
     1921 ttaataatta gaaataaagt acattaagtt ttaagaaaaa ccataaattt gaaatccaat
     1981 attaatatat cacgattatt aaaaaaagca agcaaaaaaa gcccaaacgg gcttttttat
     2041 caattttatt tatggtaatc gtaaaattta ctatattacc ctcaggaaca ttgaaattta
     2101 cgaagtttcc atattaaaaa gaaatttaat agtgaaaata tggaaatacc cggaacagtt
     2161 gaaggcaaaa atggctggat aagtgcttgt tttatgataa aaaaagggtt gcgcaaaacg
     2221 tgtgaaaaaa tcactttgaa aaaacagatt aacatttttt ggtgaattat atcgtgagca
     2281 tggtcaacga gattgatcgg caggttataa tatctcttta taggtatgca gagtcgcgtt
     2341 tttataaacc ctgaatgttg tctgatgata aaagaagata ggaaataaat gatgtataag
     2401 ataattgaaa agtattctct tgcgcctcag attgagtacg caagaatcaa ggctccaatg
     2461 atatccaaaa acgcagaacc gggacagttt cttattgttg tcgcgtgtga aaagagcgaa
     2521 agaattccgc taaccattgc cgattttgat cgtgaagagg agtcggtatc gattgtattt
     2581 cagaaaatgg gtaagggtac tcacaagatt gccgacttta aggccggaga ctcttttcat
     2641 gctgtgctgg gacctcaagg aaacccgacc gagatagagg gagaaaagca tatagtcatg
     2701 attggtggcg gtatcggtgt cgcgccggtt tatccaattg cacgaaagtt tcacgaaaat
     2761 ggggttcatg ttacatccat aatcggatac aggggcaagg attttctatt ctgggacgac
     2821 aagcttaagg ctgtttcaga tgagatgata atcaccacta atgacggatc ggttggccat
     2881 aagggttttg ttaccgatgt tcttaagcag atgatcgagg acaaaaagcc tattgaccgc
     2941 gttatagcga ttgggcctcc aattatgatg aaggccgttg ccgatgttac acgtcctcat
     3001 ggaattgcta caacggttag tcttaattcg ctaatggttt gcgggatggg aatgtgcgga
     3061 gcctgccgtg ttactgttgg aggcgagacc aagtttacat gcatggatgg ccctgagttt
     3121 gacgcgcata aagttgattt tgaagagctg atgcagcggt tgggaatgta taagagtgaa
     3181 gagaaagata tgcttgatga ataccaggga gaagataatg agtgatcgtt ttaaagcaat
     3241 acatatgaaa gagcagcccg ctgaggagcg aaagaaaaat tttcttgaag ttccatgcgg
     3301 ttatacggaa gaggaggcgg ttcttgaggc taatcgttgc cttcagtgta aaaacgcacc
     3361 ttgtatagaa gggtgccccg tttcaataga tatacgttcg tttattggac atattcaaaa
     3421 acgagaattt gataaagctg ttaataagat ttacgaagct gatattttgc catgtgtgac
     3481 gggacgtgtt tgccctcagg agagccaatg tcaggcaaaa tgcgttctta ataataaagg
     3541 cgatgccgta tcaataggaa gtcttgagcg ttacgtcgcg gatcgtgatc ttgagcgtag
     3601 acgcgaggct cttaagggcg aaccggtaaa aataagcgga aagacaaaag tagccgttat
     3661 tgggtcgggt ccggccggta ttgcctgtgc tgccgatctt ggaaaatacg gctatgacgt
     3721 tacaattttt gaggcgttcc ataaggctgg cggtgtgctt gtgtatggta ttcccgaatt
     3781 ccgtttgcca aaggatattg ttgatgtcga gattcgctca cttgttcaga ccggtgtaaa
     3841 acttgtgaca aacgttttga caggacggtc gtttactata gatgaactta tggctgaagg
     3901 ttataaggcc gtatttgtag gctctggagc cggactgccc cagtttatga aactgcctgg
     3961 tgaaaacctg aataatgtct attcggctaa tgaattttta actcgtgtta acttgatgaa
     4021 agctcatcgt tatcctgaat atgatacacc tgtaaaatat gggaaaaact gcgcggtaat
     4081 aggtggtgga aacgttgcac tggatgtcgc gcggactgcc ttgcgtcttg gaagcgaaaa
     4141 tgtgtatctg atttaccgac gtacacagga agagatgcct gcgcgtcttg aagaggtacg
     4201 gcatgctttg gaagagggta ttcaactttt ggctttgaca aacccagtgg cctatcatgg
     4261 cgatgaagcc ggatttcttc aaaagataga gtgtgtcaag atggagcttg gtgagccgga
     4321 tgaatctgga cgccgccgcc ctgtgttggt agagggttct aatttcttta ttgaagccga
     4381 tgtttgtgtt gtttcgatag gctcgacacc gaatcctcta attgcaatga cttctccgct
     4441 tattgagaca acaaaatggg gaacaataaa ggtggatgag aaaggtatga ccacaatgcc
     4501 gggagttttt gctggtggag atattgtaac gggtgcggcc actgttatta cggcaatggg
     4561 agccggacgc acggctgcag catccatgca tgagtatttg caaaacaaga aagattaatt
     4621 ttaaaatttt aaaaaagcat taataatata caaaaacacg tgccttttgg tacgtgtttt
     4681 tttttcgata aacaccactg tcgcggagtg ccagcgacgc gaagtggtga cttaacgtaa
     4741 tctgaaattc tgaatatatg gatttcgttc taaacggttt tagacttcga gtcatttgta
     4801 ttaactgcct gtgatgacgt ggatttcgat acggcaagac ttttgaaaca tgcagatgcc
     4861 attaaaactg ataaaactga cggcaatggt aagtatgtaa ttagcattac gggtgtcgct
     4921 tttattgcca ttatgttata cggtaagggg cttgcggttg gagaagaaag aacgcattat
     4981 gattacagca aggttccgga tatcgactat atgattagcg atatcgctta gtgtggcttt
     5041 tttaccaggt aaatcagggt ttacatgacg aatcactggt ttttaagaaa cagtattgtt
     5101 ctggtattgg tttctctaca gaaaaaagaa ttgctgaata taggcccctg ccgcaaaata
     5161 atacgcgatt tgtcacaacc gcggccaaaa ggagatgtct gtgaaacggg aaagaataaa
     5221 gcatgttttg tcaattgtag aagtcggttc aaggattact gtcaagggat gggtgcgtac
     5281 ctgtcgtaag gtaaaggatc tgatttttat agagataaat gacggctcgg atatagaatc
     5341 attgcaggtg gttgccgaac caaaaataga agaatataag caactttccg aattaacaac
     5401 tggatcctcc ttgagtgtga cgggtacgct tgtcgaatcg ccggccgctg gacaaagagt
     5461 tgaacttaat gccgaagaag cagaaattta tggcttaact gatgcatctt cctatccctt
     5521 gcagaaaaaa aggcattctt ttgaattttt gagagagatc gcgcatttac gcccgcgcac
     5581 aaacaccttt ggcgctgtct ttagggttcg aaacagcctt gcccatgcgg tacactcttt
     5641 ttttcagcaa cgtgagtttc tttatgtaaa cacaccaata attacagcta acgattgcga
     5701 aggagcgggt gaactctttc atgtaacaac ccttgacccc cttaaacaga ataaagacta
     5761 tggcgatgat tttttcggta agaaggcatc tttaacggta agcggacagt tagaaggaga
     5821 actttttgct acagctcttg accggattta tacatttggc cccaccttca gggccgagaa
     5881 ttcaaataca tcgcggcatc tcgcggaatt ctggatggtt gagcccgaag ctgcttttaa
     5941 tgacattaat gacaatatgg atcttgccga agatttctta aaacatatta ttttatcggt
     6001 tttagatgaa tgtcccaagg atatggattt ttttgataag aggataaagc cggggcttat
     6061 aaagaatctt gagaaaattg tctcttcgaa gtttgaaaga atgacttata cagaagcatt
     6121 aaagactttg attgattcaa aagagaaatt taattacaag gtagagtggg gttgtgacct
     6181 tcagtcagag catgagcgtt ttttggtaga aaaaaagata ggatcgccga ttattctgac
     6241 tgattacccg agaacattga agccttttta catgtattgc aacagtgaca ataaaacagt
     6301 aagaggaatg gatgttcttg tgccgggtgt tggcgagata ataggtggaa gtgaacgtga
     6361 atatcggctt gatcttctac ttgacagaat gaaagaactg aaccttaatg aagaggatta
     6421 cgattggtat attgatacaa gacgttttgg tacagtgcct cattcgggtt ttgggcttgg
     6481 ttttgaaaga gcggttcaat ttgttaccgg gatggaaaac attagagatg ttattccatt
     6541 cgcacgtact ccaaaaaatc ttaaatttta gaggaaatat gaaaattgca ataggttcgg
     6601 atcatacagg agttgatctt aaagctctta taattaaaac ttttgatgat gtcgacttta
     6661 tggatttcgg ccctgaagca aaggaatcgg ttgattatcc tgattatatc gagaaggtcg
     6721 ccaaagctgt acagaaaggg gaatgcgaac gaggcattgc tatttgtgga acaggcatag
     6781 gagcaagcat aacggcaaac aaggtgcgcg gcatacgggc ttctctatgt tttaacggat
     6841 ttatggccga aatgacccgt aaacacaacg actccaatgt cttggttttg ggagcgcgtg
     6901 ttacgggaga agaggttgcc ttgtatattg tggaaaaatg gctaaacaca ggatacgagg
     6961 gcggacgtca tcagcgtagg cttgataagg tatccgctat tgaaaatcgc gagattggtg
     7021 gagattgatt ttgcgacggt ttgctatctt cactgcaaca ataattgttc ttttttcaat
     7081 ggttactctc tattcttcaa agagagtaac aggaatatat gatttcactc ctgaaagact
     7141 tcattttcat cgtgtatctc caatcaaaac agaaaaaggt tttgaatata ttatttcaga
     7201 aacaacctac gaacgccccg actatcttat cagtgatctg actgtatcgt ttaataaaaa
     7261 aaattatcac tcaatggatg attccggaaa ataccggaca tcggtttcga gttattcctg
     7321 gtttaagccc gagggaagtg ccggtgatgg agcagggtat ttttacaaac gtaatcatcg
     7381 actcgaattt agtaatagct cggcttcatg gcttggaaat aacggtgatt tgggctcttt
     7441 tactattgaa tttcgtctaa accctgcatc gtttaacgat gaggcggagc tcttttcaag
     7501 agttggttac aactcgggaa cacgcaatgg aattctaata cagattaaag atcgtcgtat
     7561 acattgttca ttttttgatc tttttataga tgataaaggt cagcattata cttatgaatt
     7621 cgcttctgca gccaagcttc aaaataaaac gtggtattac acagctcttt cgtatgatag
     7681 gctaaccggc aaggtagaac tttttataga cgggaaaagc caggttatgt tttatgcgac
     7741 tgatgacggt acatcttcag gaaccttaca taccccttcg ttttttaaaa acgacatgcc
     7801 caatattgtc ttcggcaaaa attattttgg ttatttggat gaatttcgtc ttagttaccg
     7861 ttcgttcgat gatcttaaaa aattgacagc tctggccgac aagaagcata tcgatgtagg
     7921 tttaacgggt agagttcctg taaatagcga aggagttgta acaagtccag tttatgagtt
     7981 tcagcataca ggtaccatga taaaaaattt tgtatgggat caggttcttg acaaggagac
     8041 ttttatctgg tttgaaatac gtctttcaga tgacaagttt gatcagtatc atcagaatcc
     8101 ccgttggtat agaataacaa ataatcagcg caatatctat ttgatgaaaa cagcaaatct
     8161 ctggttgcga ggcaaatatt ttcaatggcg ggcacatctg atatcttctc ctgatggcga
     8221 aagatcccct gtgcttcaat cgataaaaat gaactatgaa cttgatacgc cgccgattgc
     8281 tccgcttttc cccgaatgta tagaacaggg cgacggttat gttgttttga gatggaaaaa
     8341 aaatgtggat catgattttt ttggttataa ggtctattat ggggttaaaa gcggaaagta
     8401 tgagggcgtt cttgtatctg atgggtcaaa acctataact aacggtagcg gcgattttgt
     8461 tgagcttaag attgataacc ggcttgtgga ggagaatagg cttaaggaca ataaaggttt
     8521 gctctcttac ccacttttaa agaataatgt gctttactat tttgctgtta ctgcgtatga
     8581 cagttaccgg gttaacacat cttataatca tgaatctgat ttttcaaaag agatattcgg
     8641 caggcctttt ggtggtagtg aaataattgt agaatcaaaa taattacgaa tggaagaaat
     8701 atggaagaaa acaaactccg aaatataata tccgaccttg agattgttaa cagaaacttg
     8761 caacttgtat cgcaaaataa atgccttgat gatagcgaaa gtggactggt aagagaagct
     8821 tcgggtctta ctaaacgggc gattcagctt ttaaacgaag aagaagttcg tatggagata
     8881 tataagctga gtgataacga ctgctagtct tggttgcgtt ttatgcaaat catggatatg
     8941 tcctcgaaag ttctgagctt atgagagaaa agagaagtct gctggattat tgtatcaagt
     9001 atc
//
LOCUS       DAOTGZ010000103         8997 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_3064
            NODE_9253_length_8997_cov_4.708007, whole genome shotgun sequence.
ACCESSION   DAOTGZ010000103 DAOTGZ010000000
VERSION     DAOTGZ010000103.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868088
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 8997)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8997)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:22:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,785
            CDSs (total)                      :: 2,751
            Genes (coding)                    :: 2,732
            CDSs (with protein)               :: 2,732
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 12 of 19
            Pseudo Genes (internal stop)      :: 5 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8997
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9253_length_8997_cov_4.708007"
                     /isolate="AMR_MDS_3064"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            90..1187
                     /locus_tag="P5123_07935"
     CDS             90..1187
                     /locus_tag="P5123_07935"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRX16227.1"
                     /translation="MRTITKLSLMSLLVAFVSVAMLLFTSSSPDGLPEAGLTTPASDS
                     SEMSETAELLDVPEKEISDVSTLQLEASITPPAPIEETTPVTESESVLVKKGILPKPG
                     EPILLSADFFTQNHVIAYYGHPRSKMMGILGRYPIDELAKRLTKTASKYDAANGNKGV
                     VTAFYLIYGVCHPKGEIGHMNDALVEKYIKFAQENGMLVFLDHQIGKYSVDYAVKKLL
                     PFLKYPNVHLAIDPEWRTLKPMKEIGSIYAHELNDAQKLMRDYMLENGIKGYRMLVVH
                     QFKPVMIKQRDSVKANYDPVVLIHNADGFGSPGAKRSAYQQNSYAKNMPFKGFKLFYY
                     SGYKGAGFDKPLMTPEQVLKLKPEPCIIMYQ"
     gene            1212..3593
                     /locus_tag="P5123_07940"
     CDS             1212..3593
                     /locus_tag="P5123_07940"
                     /inference="COORDINATES: protein motif:HMM:NF013316.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="U32 family peptidase"
                     /protein_id="HRX16228.1"
                     /translation="MFCNDKKIPCLLAPGGDYDAVIAAFVAGADEVYCGVDRFNARRR
                     ASNITLAQLNKLASMARRSDRHIYLTINTLCVQNEINDIIELIGHALNSGVHGLIIQD
                     YGLLYIVRTLFPDAVIHASTQMTTLNKGQIEFLSECGVSRVNLSRELDIDTIEELTLF
                     THSLGMQTEVFVHGAYCISYSGQCYMSSFMGGQSGNRGICFQPCRRSYQIAEERKNIY
                     PLSLKDNSAFAFAKQLSDAGVDSLKIEGRIKGFMYVYNTVSAWRGQLDKIEKGQPLLE
                     DDRRLHSVFNRGFSAGYLLGEVSSDMFTNRQADQSLRFAGTVTTYHADKKIITISSER
                     SGCKIKSGDRVLLYSSENRFICTGIIGNQTGDLSYGFTIENELKDKINRGQLVYISGG
                     QPEREKVLNIINSFEAPLKNLSVTISGKKGDVLKAKWLCEGREFNCATDDILEEAKSI
                     SLGEEVLQKQLSKLSDSGFVLKIIDVSALDPGLYIALSQLNMLRRKALNFFLGYKEYN
                     GLDLKIPNKKNKIRREGNVSYLINSHDHYDIIRESGLPFGNIYFEVTDPGVYYNNDDI
                     IPWFPSVISQKNMNDYVSLIDSLPNNARVVINSSSLVSNIRKRGLKWIAGPYFNITNS
                     MALELFANYDSCFDGGFVSMELNREQINDLAYETDHPLYMMIFGPLLLMSTRQCLIRQ
                     AAGCSKQYVDSICFKTCSKNIAIENQNGDKYNLVKRQFMPQEMYNGAYLSIPEAIKEI
                     DNIDFCVDIREIPSIGYSNDIRKKIVNTLSRKLRNLELSEIKTTSGNYRRGLS"
     gene            complement(3597..7055)
                     /locus_tag="P5123_07945"
     CDS             complement(3597..7055)
                     /locus_tag="P5123_07945"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: protein motif:HMM:NF005298.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit alpha"
                     /protein_id="HRX16229.1"
                     /translation="MGFVHLHNHTDYSALDGAITVKKLIERTADYGMTAVAISDHGNM
                     CGAIDFYETARKKGIKPIIAQEFYIAPDSRFNRKYSKGENTNYHLMLYAKNNTGYLNL
                     LKLSSIGYLDGFYYKPRIDFEVLEQYKEGLICSTACLAGEIPQKILSKNMDAARETAK
                     IYQKLFGEDHFYLEMQDHGLPEQQIVNEGLVQLSKELSLPLIATNDCHYTDKDDAYAH
                     EVLLCIQTGKTMEDENRMRFGTNEFYFKKPEDMERLFSNYPDAIHNTQKIADMCDVEL
                     KLNNPVLPTFTVPDGFTLDSYMRELVYKGAAERYGKELSEKVKEQIEYELGVITNMGF
                     SGYFLIVWDFINHARKKGIPVGPGRGSAAGSIVSYCMGITQLNPLDYNLLFERFLNPD
                     RNEMPDMDIDFCGVRREEIIQYVRDKYGDDHVSQIMAFNTLKPKAAVKDVARALSVPF
                     TEANRITKYINEKTIMESVEKSPELKEFYNSSANSRQVIDIAVKLEGLIRSFGKHAAG
                     VVISKEEITHYVPLYKDSKDGSICAQFEKKNSEAAGLVKMDFLGLKNLTIINHALCLI
                     KQHQGVDLDIETLPIDDQKVYKLLQNADTNGIFQLESSGMQNLLRKLGPTVFDDIIAV
                     GALYRPGPLNSGMADQFIKRKRDPKLIEYPHECLEPVLKDTLGVIIYQEQVMKISQVM
                     GGFSMSEADKLRKAMGKKLDHIVEQLRGKFLEGAEKQKIDPKIAETVYDAMAKFAEYG
                     FNKSHAAAYGLVTYQTAYLKTHYRAEYMCALLTAAKDNQDDITKYIADCRTAGITVLP
                     PDINQSDYSFTIEVDKIRYGFCAIKGLGERAIEEILKTRDKIGGFSSLQQLIENLDLS
                     VINKGVLESLIKSGSLISLNKNRAQLFAGIDTILETARFLQKDKKSGQGNLFGGDEND
                     DSEEMINLPDLPDWQDHVALNFEKETLGLYITGHPLARYEKELREYSSCSIINIDETS
                     NGKSKTYSIVGILENVRTHITKKGATMAFAMLEDMEGSIEVVIFPRTYKKYQHMVSLN
                     EPVMLTGEVEISEDEDSDYRSKKMLVSEIRTLAEVRNRSISAVHLNINTIGFDEMIME
                     KIRDLAQSNHGSCPLFFNIELENGQNALIRTHNCFNIEPTENVIHSLKSILGDESIHY
                     AFKNVC"
     gene            7383..8066
                     /locus_tag="P5123_07950"
     CDS             7383..8066
                     /locus_tag="P5123_07950"
                     /inference="COORDINATES: protein motif:HMM:NF019260.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1593 domain-containing protein"
                     /protein_id="HRX16230.1"
                     /translation="MKKLILLFMIMISISFISCEDAKESDDKTVQQNEGKENGTESGV
                     DNDANPDNDDSGENGTNSNNNPAEDPIENPTENPVEIVIENPSLLKKPRIVVMADIMP
                     LPPEGEEPKHEVDDVQSFIRLLVYSNEFEIIGLIGANSRYGVNRGDSEAFYGLIDLYE
                     QIKPNLDKHAKGFPTAENLRKVVFEGQRTYLGWLVSVKIKIQLVQTIFCLYLKIRIMN
                     RYGFLDGGA"
     gene            7958..>8997
                     /locus_tag="P5123_07955"
     CDS             7958..>8997
                     /locus_tag="P5123_07955"
                     /inference="COORDINATES: protein motif:HMM:NF019260.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1593 domain-containing protein"
                     /protein_id="HRX16231.1"
                     /translation="MAGIGEDKDTAGSNHILSLLKDKNNEPIWFLGWGGMNTLMQAIW
                     KIEKMVKDETISEDEKARMLEKIRIFDIAAQDNSCSEIAKTYPDIFYVRSTHQFFSFS
                     KWKYNFNAPEKDSQKGDLSFVNHTWFRDNVQDSKFGDFGKEYPDYRCLYEGDTPSFLY
                     LIQNGLSDPMKPHYGSWAGRFSRNREYSRGAKHNNKYDGDGYVYTDEHESVKDGFEGV
                     NDVYACLWRWREDYQNDFAARMLWTISDSFNKANHNPVVIVNGDETKSVLHVKVQKNR
                     FITLEAGSSYDPDSDTLNYSWWQYKEPGTYRGSVSIEDNTTDTVKVTVPADANSGDTI
                     HIVLTVRDNGDPV"
BASE COUNT         2741 a         1649 c         1837 g         2770 t
ORIGIN      
        1 aggtaacaac aaaattgcac gatactcata atatgataac ctgaaaaact ggtactaaaa
       61 tttattaatt atatgattaa cggaatgaaa tgagaacaat tactaaacta tcattaatgt
      121 ctttgcttgt cgcatttgtg tctgtggcaa tgcttttatt tacatcatct agcccggatg
      181 gccttcctga agcaggtctt acaaccccgg catctgattc aagcgaaatg tccgaaacag
      241 ccgaacttct tgatgttccc gagaaagaga tcagtgatgt ttcgacattg caactcgagg
      301 caagtattac tcctccggca cctattgaag agactactcc ggtaactgaa tctgaatcgg
      361 tccttgttaa aaaaggtatt cttccaaaac cgggagaacc cattctatta tccgccgatt
      421 tttttaccca gaatcacgtt atagcctatt atggccatcc tcgttcgaaa atgatgggaa
      481 tccttggtcg ctatccaata gatgagctgg caaagcgact taccaaaaca gcttcaaaat
      541 acgatgccgc taacggaaac aagggtgttg ttactgcttt ttaccttatt tatggtgttt
      601 gtcaccccaa aggtgaaatt gggcatatga acgacgccct ggtagaaaaa tatataaaat
      661 tcgctcaaga aaatggcatg cttgtatttc ttgatcatca gattggcaaa tacagcgtcg
      721 attatgcagt taaaaaactt ctaccttttt taaaataccc gaatgtgcat ctggcaattg
      781 atccggagtg gcgtacgtta aaacccatga aagagattgg cagtatctat gctcatgaat
      841 taaacgatgc tcagaaactg atgcgtgatt atatgcttga aaatggaata aaagggtatc
      901 gaatgcttgt tgtacatcaa tttaaaccgg ttatgataaa gcaaagagac agtgtgaagg
      961 ctaattatga tcctgttgta ttaattcata atgctgacgg attcggctca ccaggagcaa
     1021 aacgtagcgc ttatcaacaa aactcttacg cgaagaatat gcctttcaag ggatttaagc
     1081 ttttttacta ttctggatac aagggcgcgg gttttgataa accacttatg actcccgagc
     1141 aggttttaaa actaaaaccc gagccttgta taataatgta tcagtaaaaa gataattata
     1201 aatggataaa tgtgttctgt aacgataaga aaataccgtg tctgctggct cccggcggcg
     1261 actacgatgc tgtaattgcc gcctttgttg ccggagcgga tgaggtctac tgtggagtag
     1321 acaggtttaa tgcgcgccga cgcgcgtcaa atattactct tgctcaactt aataaactcg
     1381 catcgatggc aagacgatcc gatcggcata tatatctgac tatcaatacc ctctgtgtac
     1441 aaaacgagat taacgatatt attgaactta ttgggcatgc tcttaattcc ggggttcatg
     1501 gacttataat acaggattat ggtctgttat atattgttag aacactcttt cctgatgcag
     1561 taatacatgc ttcaactcaa atgacaacct taaacaaggg acaaatagaa ttcctctccg
     1621 aatgtggggt ttctcgggtt aatctttcac gtgagcttga tatagataca attgaagagc
     1681 tgaccttatt tacgcactcg ctgggtatgc aaaccgaggt attcgttcac ggagcatact
     1741 gtatctctta ttctggccaa tgttatatga gttcgtttat gggagggcag tcgggaaaca
     1801 ggggaatctg ttttcagcca tgtcgccgat cataccaaat tgctgaagaa agaaaaaata
     1861 tttatccact aagtttaaaa gataacagtg cctttgcttt cgctaaacag ctttcggatg
     1921 ccggggtcga ttctttaaag atagaaggtc gtataaaggg ctttatgtat gtctataata
     1981 cggtatctgc ttggagaggg cagcttgata aaatagaaaa ggggcagcca cttttagaag
     2041 atgatcgcag gttgcattct gtatttaatc gtggatttag tgccggatat cttttgggcg
     2101 aagtttcatc ggatatgttc actaacagac aagctgacca atcacttaga tttgccggaa
     2161 ctgtaacaac ttatcatgcc gacaagaaaa taataactat tagctctgaa agatcaggat
     2221 gtaaaattaa atcaggagat cgggttctat tatattcttc tgaaaaccgc tttatatgta
     2281 cagggataat aggaaatcaa actggcgacc tatcgtatgg gtttaccata gaaaacgagc
     2341 ttaaagataa gattaacaga gggcagcttg tctatatatc tggggggcaa cccgaacggg
     2401 aaaaggtatt aaatattata aattcttttg aggctccctt aaaaaattta tcggttacta
     2461 tttccggaaa aaaaggagat gttcttaaag caaaatggtt gtgtgaaggg cgcgaattta
     2521 actgtgctac cgatgatata ctggaagagg caaagagtat ttcccttggt gaagaggttt
     2581 tgcaaaaaca actttcaaaa ctatcagata gtgggtttgt tcttaaaatt atagatgtct
     2641 cggctttgga tccagggtta tatattgcct tgtcacagct taatatgctt agaaggaagg
     2701 ctctcaattt ttttcttggt tataaagagt ataacggttt agatttaaaa attccaaata
     2761 aaaaaaataa aattcgcaga gaaggtaatg tttcgtatct gataaacagt catgatcatt
     2821 acgatataat acgtgaaagc ggtttgcctt ttggaaatat ctattttgaa gttaccgatc
     2881 ctggagtgta ctacaataac gatgatatta tcccctggtt cccttctgtt attagtcaaa
     2941 aaaacatgaa tgactatgtt tcactgatcg attcattacc aaataatgca cgtgttgtaa
     3001 ttaacagtag ctctttagtt tctaatataa gaaaacgcgg attaaaatgg atagcgggtc
     3061 catactttaa tattacaaac agtatggcat tagagctttt tgcgaactac gacagctgct
     3121 ttgatggggg cttcgtttca atggagctta atcgtgaaca gattaacgat ttggcttatg
     3181 aaacggatca cccactatac atgatgattt tcggtcctct tcttctgatg tctacacgtc
     3241 aatgtcttat tcgccaagca gcaggctgtt caaaacagta tgttgatagc atttgtttta
     3301 aaacctgttc aaagaatatc gcgatagaaa atcaaaacgg tgataaatac aatctggtta
     3361 aaagacaatt tatgccacaa gagatgtata atggtgcata tctttcgata ccggaggcaa
     3421 taaaggaaat tgataatata gatttttgcg ttgatatcag agaaatacca tctatcggat
     3481 actcaaacga tatacgaaaa aaaatagtta ataccttgtc acgaaaactg cgcaatcttg
     3541 aattgagcga aataaaaaca acatcgggta attaccgccg tggtttatca tgatacttaa
     3601 cagacgttct taaaagcata gtgtatcgat tcgtcaccaa gaatggattt taaagagtgt
     3661 ataacgtttt cagtcggttc aatattaaaa cagttatgag ttcttattaa tgcgttttgc
     3721 ccgttttcca attcaatatt aaaaaacaga ggacagctac catgattgct ctgtgccaag
     3781 tcacgtatct tttccattat catttcatca aaaccgatgg tattgatatt aagatgtaca
     3841 gccgaaatgg agcgattgcg tacttcggca agagtacgta tttcgctgac caacatcttt
     3901 tttgatctat agtcagaatc ttcatcttca gatatttcaa cttcaccagt aagcataacc
     3961 ggctcgttaa gtgacaccat atgctgatac ttcttatagg ttctgggaaa aatcacaact
     4021 tcaattgatc cttccatatc ttcaagcatg gcaaaagcca tagttgctcc cttttttgtg
     4081 atatgagttc ttacattttc gagtatgccc acgatcgaat aagttttact cttgccatta
     4141 gatgtctcat cgatatttat tatggaacac gaagagtatt ctcggagctc tttttcataa
     4201 cgggcaagag gatgtcccgt aatatagagt cctagtgtct ctttctcaaa gttcaaagcg
     4261 acatggtctt gccaatctgg taagtctggc aaattgatca tctcttcaga atcatcattc
     4321 tcgtctcctc caaaaagatt cccctgtccg ctttttttat ccttttgcaa gaagcgggcg
     4381 gtttcaagta tggtgtcaat tcctgcaaaa agttgcgcgc gatttttgtt aagtgaaata
     4441 agtgatcctg atttaattag tgattcaaga acccctttgt tgataacaga gaggtcaagg
     4501 ttttcaataa gctgttgtaa agaagaaaaa cctccaattt tatctctggt ttttaatatc
     4561 tcttctattg cacgttcgcc taatcccttg atggcacaaa atccgtaacg tatcttgtct
     4621 acttcgattg taaaactgta atcactctgg tttatgtcgg gtggaagcac cgttatgcct
     4681 gcggtacggc aatcagcgat atatttggta atgtcatctt gattatcctt tgcggcagtt
     4741 aaaagggcgc acatatattc ggctctgtag tgggtcttca gatatgcggt ctgataggtt
     4801 acaaggccgt atgctgcggc atgcgatttg ttaaaaccat attccgcaaa ttttgccatc
     4861 gcatcatata ctgtttccgc gattttagga tcaatcttct gtttttcggc gccttcaaga
     4921 aattttcctc gaagctgctc tactatatga tccagcttct tgcccattgc cttacgtaat
     4981 ttgtcagctt cgctcattga aaacccgccc ataacctgag agatcttcat aacttgctct
     5041 tggtagatta ttacacccaa ggtatccttt agaaccggtt caagacactc atgtggatat
     5101 tcgattagtt ttggatcacg ttttcttttt ataaactgat cggccattcc cgaatttagg
     5161 ggacccgggc ggtacagtgc gccaacggca ataatatcgt caaagacagt tggccccagt
     5221 tttcttaaaa ggttttgcat accggaactt tcaagctgaa aaattccgtt tgtgtccgcg
     5281 ttttgcagta acttgtaaac tttctgatca tcaataggaa gtgtctcaat atccagatcg
     5341 actccctgat gttgtttgat aaggcaaagc gcgtgattta tgattgtaag gtttttcaga
     5401 ccaagaaaat ccattttaac aaggccggcc gcttccgagt ttttcttctc gaactgggcg
     5461 caaatacttc catcttttga atccttgtaa agtggaacat aatgggttat ctcttccttg
     5521 gaaataacaa ccccggctgc atgtttgcca aatgatcgaa tcagaccttc gagcttaacg
     5581 gcaatatcta taacctgacg tgaatttgcg ctcgaattgt aaaactcttt cagttcgggt
     5641 gatttttcca ccgactccat aatggttttt tcgttaatat atttagttat ccggttcgcc
     5701 tcagtaaaag ggacggacaa tgcccgagcg acatccttta cagcagcttt tggcttcagg
     5761 gtattgaagg ccataatctg tgaaacatga tcatcaccat atttatccct tacgtactgg
     5821 attatctctt cgcgtcgaac accgcaaaaa tcgatatcca tatcgggcat ctcattacga
     5881 tccggattaa gaaaacgctc gaaaagaaga ttataatcta acggattaag ctgggttatc
     5941 cccatgcagt aagagacgat tgatcctgca gcagatcctc ggccaggacc aacaggtatt
     6001 cccttttttc gagcatggtt aataaagtcc catacaatca agaagtatcc tgaaaatccc
     6061 atatttgtta ttacacctag ctcatactca atctgctctt ttaccttttc agaaagttct
     6121 ttgccgtagc gctcggcggc acctttgtaa actagctcgc gcatatagct atcaagcgta
     6181 aatccatcag gtacagtaaa agtaggaagt acagggttat ttaatttaag ctctacatcg
     6241 cacatatcag cgattttttg cgtattatgt atcgcatctg gatagttgct aaatagtctt
     6301 tccatatctt cgggtttctt gaagtaaaac tcatttgttc caaagcgcat ccggttttcg
     6361 tcttccatcg ttttaccggt ctgtatgcat aacagaactt cgtgcgcgta ggcgtcatcc
     6421 ttgtcggtat agtgacagtc gtttgtggca ataagcggca gggataactc tttggataac
     6481 tgaacaagac cttcgttaac tatctgctgt tcgggcaggc cgtgatcctg catctcaaga
     6541 taaaaatgat cttctccaaa gagtttttga tatatctttg ccgtctcacg ggcggcatcc
     6601 atattttttg agagtatttt ctgtggaatt tcgccagcaa gacaagcggt agaacagata
     6661 agtccctctt tgtactgttc gagaacctca aaatcgatac gtggtttata atagaaacca
     6721 tccagatagc caatggaaga cagtttcaga agattaagat agcctgtatt gtttttggca
     6781 taaagcataa ggtgatagtt cgtattttca cctttagagt atttacggtt aaaacggcta
     6841 tcgggagcta tataaaactc ctgtgcaata attggtttaa ttcccttttt gcgtgctgtc
     6901 tcataaaaat ctatggctcc acacatattg ccgtgatcag agatagcaac agccgtcatt
     6961 ccgtaatcgg cagttctttc tataagtttt ttaactgtta tggcaccgtc aagagccgaa
     7021 tagtcggtgt gattgtgaag atgtacaaaa cccatatcta tctccgagaa aggcaaaaaa
     7081 tttggggaat taatgaaaaa tatttgattg tctcctgttt acgtcaacga aattaactgt
     7141 ttttttataa attgcattga attaaatacg agcctatttg cttttattca tgaccacctg
     7201 tacggatttt gaaaacagag taatttgcat gtttttgaca ttattatggt ataatatccg
     7261 taaaaaccga tgaaaattgg taaaattaag tatttgtacg gatgtttttt ttagcgttat
     7321 ggaataaaaa tatggaaatt agtttgaaaa tattaaaaaa ttgggtagca agaggtaaat
     7381 aaatgaaaaa gctcatttta ctatttatga ttatgatttc gatatcattt atttcgtgcg
     7441 aagatgctaa agagagtgat gataaaacag tacaacaaaa tgaaggaaag gagaatggga
     7501 ccgaaagtgg tgttgataat gatgcaaatc cggataatga tgattccgga gaaaacggaa
     7561 ctaattccaa caataatcct gccgaagatc ctatagaaaa tcctactgaa aatccagttg
     7621 aaattgttat tgagaatccg tctttgctta aaaagcctag aattgttgta atggcagata
     7681 ttatgcctct accacctgaa ggtgaggagc ctaaacacga ggttgatgat gttcagtcat
     7741 ttataagact tttagtctat tctaacgagt ttgaaattat agggcttatc ggtgctaaca
     7801 gccgttatgg agtgaacaga ggtgattcgg aagcatttta tggtttaatt gatctttatg
     7861 agcagataaa accgaatctg gataaacacg ccaaggggtt tccaactgcc gaaaatttaa
     7921 gaaaagttgt gtttgagggg caacgtacct atttgggatg gctggtatcg gtgaagataa
     7981 agatacagct ggttcaaacc atattttgtc tttacttaaa gataagaata atgaaccgat
     8041 atggtttctt ggatgggggg gcatgaatac attgatgcag gctatctgga aaattgaaaa
     8101 aatggttaaa gatgaaacaa tttcagaaga tgaaaaagct cgtatgttag aaaagattcg
     8161 aatctttgat atagcggcac aggacaatag ttgttctgaa attgctaaaa cttatcccga
     8221 tattttttat gttcgttcaa cacatcagtt ttttagtttt tctaaatgga aatataattt
     8281 taatgcacct gaaaaagatt cacaaaaggg agatctctct tttgtgaatc atacttggtt
     8341 tcgagataat gtccaagata gtaaattcgg tgattttggc aaagagtatc ctgactatag
     8401 gtgtttgtat gaaggtgaca ccccatcgtt tttatacctg attcaaaatg gattaagtga
     8461 tcctatgaag ccccattatg gatcttgggc tggaagattc tctcgtaaca gagagtattc
     8521 aaggggggcg aaacataata ataaatatga tggggatggt tatgtatata ccgatgagca
     8581 tgagtcggtg aaggatggct ttgaaggggt aaacgatgtt tatgcttgtt tatggcgctg
     8641 gagagaggac tatcaaaacg attttgcagc tcgtatgctc tggaccatat cggatagttt
     8701 taacaaggct aaccataatc ctgttgttat agttaacggt gatgagacca aaagtgtact
     8761 tcatgtgaaa gtgcaaaaaa accgttttat aacacttgaa gcaggaagta gttatgaccc
     8821 tgattctgat acattaaact actcatggtg gcagtataaa gaacctggca catacagagg
     8881 ttcagtcagt atagaagaca ataccactga cacggtaaaa gtaacagtac ctgccgacgc
     8941 gaatagtggc gatacaatac atatagtatt gactgtgcgt gataacggtg atccggt
//
LOCUS       DAOTGZ010000104         8953 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_3064
            NODE_9318_length_8953_cov_4.524275, whole genome shotgun sequence.
ACCESSION   DAOTGZ010000104 DAOTGZ010000000
VERSION     DAOTGZ010000104.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868088
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 8953)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8953)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:22:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,785
            CDSs (total)                      :: 2,751
            Genes (coding)                    :: 2,732
            CDSs (with protein)               :: 2,732
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 12 of 19
            Pseudo Genes (internal stop)      :: 5 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8953
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9318_length_8953_cov_4.524275"
                     /isolate="AMR_MDS_3064"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            111..1652
                     /gene="rny"
                     /locus_tag="P5123_07960"
     CDS             111..1652
                     /gene="rny"
                     /locus_tag="P5123_07960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020614324.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004521 - endoribonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006402 - mRNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease Y"
                     /protein_id="HRX16232.1"
                     /translation="MTILTTILLCVSGLVIGVLSGYLIRSYKGKLSLNSAESRAQRIM
                     QDAKRDAESKRKELLLEAKDQLIQEKNLLEREMRERRQELTNVEKRIMQKEELIDTKT
                     VELEKKEREIAHRESENQKIEAELQNEFERYRKELEKIANLTQEQAKTMLLKELEDTV
                     RFESLKLINKIEEEARRTADKKAKEIVVSAIQRNATEYTSENTVTTVSLPSDDMKGRI
                     IGREGRNIRTLENLTGVDMIIDDTPEVVVISGHDPIRREIARFSLERLILNGRIHPAR
                     IEEVVEKVTKEIEENIMEEGEKAAFELGIPGLSSDALYHIGKLKYRSSYGQNVLGHSI
                     EVANIAGIIAGELKLDVQLAKRAGLLHDIGKGSVVEGEGAHAIVGSEMAKKFGENEKV
                     VNIIASHHNDKEPGSFEAYIIQIADAISASRPGARRESLDNYLKRLEDLENIANSFTG
                     VDKSFAIQAGREIRVIVANDIVNDEKAEFLAREIAARIESELKYPGIVRVIVIRETRV
                     LEYAK"
     gene            1795..3681
                     /gene="dxs"
                     /locus_tag="P5123_07965"
     CDS             1795..3681
                     /gene="dxs"
                     /locus_tag="P5123_07965"
                     /EC_number="2.2.1.7"
                     /inference="COORDINATES: protein motif:HMM:NF003933.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008661 - 1-deoxy-D-xylulose-5-phosphate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0008615 - pyridoxine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009240 - isopentenyl diphosphate
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-deoxy-D-xylulose-5-phosphate synthase"
                     /protein_id="HRX16233.1"
                     /translation="MDKYVLLEKINTPNDLKKLSADQLCKLAEEIRDEIINVVSVNGG
                     HLASSLGVVELSIALHYVFDTPVDRVIWDVGHQSYAHKLLTGRRDIFSTLRQFGGMSG
                     FPKISESEYDCYNVGHSSTSLSLAAGEICARDLKGENYAVVPVIGDGSMTSGMAFEAL
                     NHIGHIKRDVIIVLNDNEHSISPNVGALSEYFTDIITGKFYNRTRRQIYRFFRSIPLI
                     GRAVERLAQTTKKFIKDIITPGKLFEDLGIRYFGPVDGHNLTHLIEIFNRVKQINNGP
                     KIIHVRTIKGKGFCPAENCPEKYHGIAPFNRETGKPLKLKTRSLSEVAGHKLCELAGK
                     DDSIVAITAAMTSGTGLDEFSKKYPRRFFDVGIAEQHAVTFATAMAKNGLKPFFAVYS
                     TFLQRSYDQLVHDAALMHLPVCLLVDRAGIVGDDGETHHGLFDVGFMRGIPGLRIFTP
                     ADETQLEKLIEYVVVNPDGPSAIRYPRGASDNYPTMVTDSFDPFGVDIDNRGDDILII
                     TAGFKRKYALEVSNILTKKNIGCTVLNLHAIAPIATGIISKNIVRCRNYCVLEDHYIT
                     GGLSEAVTAVLESELRVKFLMNFGYPAEFICHGTSDTIENKYGLLPSQIAESIYQGFN
                     GKNR"
     gene            3665..4465
                     /locus_tag="P5123_07970"
     CDS             3665..4465
                     /locus_tag="P5123_07970"
                     /inference="COORDINATES: protein motif:HMM:TIGR00478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TlyA family RNA methyltransferase"
                     /protein_id="HRX16234.1"
                     /translation="MERIDKHLAQSGLSSTREQAKKEILAGWVKINGETCLDVSRKVE
                     GIENITVSRPKGLFVSRGGYKLEKALNYFNIDVSGLTVLDLGASTGGFSDCMLKRGAS
                     FVYAVDVGYGQLDYSLRIDKRVEVMERTNARNLTIENFEKGIPTFVSADLSFISFTKV
                     FPVIRGLFKDIKGVALIKPQFEAQRGEHKKGVVKSSSVHLDILTRVLTILSDQGCSLS
                     GLTHSPVTGPKGNIEFLLYFSLVEDSSFDLSSIEDIVKCAWDELKEKS"
     gene            4462..4818
                     /locus_tag="P5123_07975"
     CDS             4462..4818
                     /locus_tag="P5123_07975"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRX16235.1"
                     /translation="MKIITIIAVVICFGSLISASEESIIAYPVPFNPGTTSLTIADKS
                     SSFTGVPIRINIDIFDICGDKIFNGSYNSFPVLWKGYSSGGKRVSNGLYIVKVTVENL
                     STGSIEKKVIRILVKK"
     gene            4815..5864
                     /locus_tag="P5123_07980"
     CDS             4815..5864
                     /locus_tag="P5123_07980"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRX16236.1"
                     /translation="MKIAISLKVCLRYFLWVNVHICFLLIISTFSIFADSTGVRAAFS
                     RLGIAGAHNLAMGGAVEAVTDDVFSVFWNPAGLTQLRSRKKSIREDVRTKLQQNQIDK
                     ITERDLKRLSDDTPKFFFQLGAAASKLQEDREAAFVAGAFNLGNTVLGMGVLGIYSDK
                     IATYSNEGVFTGDTSYQAGVSYLSVAYPTEYVSIGVTAKTFYENIDRTNFAGGAFDAG
                     IQTDFIPLVQIGVVVQDIGVGLMPIEKDDEVKNEYEFGKPVFRFNIALVSRTANMVVS
                     AGIIKKFEDKSSDYKLGIRYYLLSNFAFMLGVNESLFTTGTMINFFDADFSYAVSVDN
                     IDMGYNHTVSFSYLF"
     gene            5881..6681
                     /gene="folP"
                     /locus_tag="P5123_07985"
     CDS             5881..6681
                     /gene="folP"
                     /locus_tag="P5123_07985"
                     /EC_number="2.5.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007419096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004156 - dihydropteroate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009396 - folic acid-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydropteroate synthase"
                     /protein_id="HRX16237.1"
                     /translation="MSFFSDIKIMGIVNVTPDSFYDGGRYNDLSKAVDHAMCLVRDGA
                     DILDIGGESTRPGAAPVSESEEMERVIPLIERLSAICDVTLSVDTNKSSVAEAALNAG
                     ASLVNDISAMTFDKGMARVISEKNAGVVLMHTSGRPDVMMHKTEYNNLVADIKSFLLE
                     RANYAQKCGIEKNKIIIDPGIGFGKTIEGNYNILSSLDEFISTDFPVLIGLSRKSLIA
                     KLYKEDEDRLPATIALNTMALSKGAAIIRVHDVKEHKLALDALKMLRL"
     gene            6696..7505
                     /gene="cdaA"
                     /locus_tag="P5123_07990"
     CDS             6696..7505
                     /gene="cdaA"
                     /locus_tag="P5123_07990"
                     /EC_number="2.7.7.85"
                     /inference="COORDINATES: protein motif:HMM:TIGR00159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004016 - adenylate cyclase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diadenylate cyclase CdaA"
                     /protein_id="HRX16238.1"
                     /translation="MELFFYNLVYILSIIWDYLRNIIDIVLVAVIFYIIYTYLVKTKA
                     IQLLRGFLFVAVLWLLARIFHFETLYWLITAMTSYFFIAIIILFQPELRRVISRFGQS
                     GWLANIDPEDSLHLDELVNAVAAMAEARIGSLIVFERDNSLRSYADSGVVINAGITEE
                     LIRTIFFPNTELHDGALIIQQGKIVSAACYLPLSDSRQLRKNHGARHRAGLGIAEETD
                     ALVVLTSEETGQIAIMVNSKLFPRIKREELKNIILFFMNPKTAYEERYSIK"
     gene            7502..8497
                     /locus_tag="P5123_07995"
     CDS             7502..8497
                     /locus_tag="P5123_07995"
                     /inference="COORDINATES: protein motif:HMM:NF019561.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CdaR family protein"
                     /protein_id="HRX16239.1"
                     /translation="MISQKEMIIDFIKSKDFIAKIVCLILAVILWSYIGNQGNSDVTL
                     RIKAEYKNLSSEYVVSDIQKTHVNVKISGREDELDAFNKNDIRVFVDFKNAVPDNRYR
                     YDVELVSSPDIPRGIDIKLMDKSIFATVEKKVSKTVFIEPVIAGQVRSGYFAGNVRAQ
                     PDRVVVYGSPEQVQKIDLIKTMPLSVAHASDNVSNEVLLDTDGYSFTEISSDKTLVTA
                     EVFSAQNVVKYEREIDLFGKMDNYLYEIHPDKKITVYIRSKDGSVPQLDINDVRVWTD
                     LSNIDSSKFITSDGNIISSLRVTSRVRAVINNRTDSVQIINIVPDVVTIRIKNEN"
     gene            8531..8911
                     /locus_tag="P5123_08000"
     CDS             8531..8911
                     /locus_tag="P5123_08000"
                     /EC_number="2.6.99.2"
                     /inference="COORDINATES: protein motif:HMM:NF015685.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0033856 - pyridoxine 5'-phosphate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0008615 - pyridoxine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxine 5'-phosphate synthase"
                     /protein_id="HRX16240.1"
                     /translation="MKYPVLGVNIDHVATLRQARGVGYPDVVDAAKICEESDIFCITA
                     HLREDRRHIQDSDIYELKKSIRTRLNMEMAIAPDVVKIALEVNPYMVTIVPEKREELT
                     TEGGLNLSILKDDLKNVIASFDKK"
BASE COUNT         2755 a         1416 c         1965 g         2817 t
ORIGIN      
        1 tctcttctct tgtccccgat tgtattattc cccgattaat gaaaaggctt tgttcggtct
       61 agtattgttg ctgtctgaac agcttaaatt gataatatca gggggaaacc atgacaattt
      121 taactactat cctcctttgt gtatcgggtt tggttatagg tgtattatcc ggttatctga
      181 tacgttcgta taaaggaaaa ctaagtctta attctgcaga gtcaagagct cagcgtataa
      241 tgcaggatgc aaagcgagat gctgagtcta aacgtaaaga actgcttctt gaagcaaaag
      301 atcaactgat tcaggagaaa aaccttcttg aacgtgagat gcgggaacga cgacaggaac
      361 ttaccaatgt cgaaaaacgt atcatgcaaa aagaggagtt aattgataca aaaactgttg
      421 aattggaaaa aaaagaacgt gaaattgctc accgtgaaag tgaaaaccag aaaattgagg
      481 ctgaacttca gaatgagttt gaacgttatc gtaaggagct tgaaaaaata gccaacctga
      541 cacaggaaca ggcaaaaaca atgctgctta aagaactaga agatacagtt cgtttcgagt
      601 cattaaagct catcaacaaa attgaagaag aggcaaggcg cacagctgat aaaaaggcta
      661 aagagattgt tgtatccgcg attcagcgta atgctaccga atacacttcg gaaaatactg
      721 ttacaactgt ttccctgcct tctgatgata tgaaaggaag aattatcgga cgagagggtc
      781 gtaatattcg tacccttgag aatcttaccg gtgttgatat gattatcgat gatactcctg
      841 aggttgttgt tatttctggg catgatccta tccgtcgtga gattgcgaga ttctcacttg
      901 aacgacttat acttaacgga agaattcatc ctgcccgcat agaggaagtc gttgagaagg
      961 taacaaaaga gatagaagaa aatattatgg aagagggtga aaaggctgct ttcgaacttg
     1021 gaataccagg tcttagcagt gatgctcttt accatatagg aaaactgaaa tacagaagta
     1081 gttatggtca gaacgtatta ggtcatagta ttgaagttgc caatatagct ggtattattg
     1141 caggtgagct taagcttgat gtacagcttg ctaaacgagc tggtctattg catgatattg
     1201 gaaagggaag tgttgttgag ggagaaggtg ctcatgcgat tgttggttca gaaatggcaa
     1261 aaaaattcgg tgaaaacgaa aaggttgtga atattattgc ttcacatcat aatgataaag
     1321 agcctggttc gttcgaggct tatattattc aaattgcgga tgcaatatcg gcatctcgac
     1381 caggtgcacg cagagagtct ttggacaatt atcttaagag gctggaagat cttgaaaata
     1441 tcgcaaacag ctttactggt gtagataaat cttttgcaat tcaggccggt cgagaaatac
     1501 gagttattgt tgccaacgat attgtaaatg atgaaaaagc cgagtttcta gcgcgtgaaa
     1561 ttgctgcgcg tattgaatca gagcttaaat acccgggtat tgtgcgagtc attgtaattc
     1621 gcgagactcg agttcttgag tacgctaaat agatacttgt aattatgcat gcgctttgcg
     1681 catgtataat taaaaatatt gatcggaata atataataag tttaaatata atagacgtaa
     1741 acttaactgt tttaaaaata tctgtatctc ttcgttgtaa gtgatacgga tgtaatggat
     1801 aaatatgtgc ttcttgaaaa gattaacacc ccaaacgatc taaaaaagct ttcagctgat
     1861 cagctttgta aacttgcaga agaaattcgc gatgagataa tcaatgttgt atcggtaaat
     1921 ggcggccacc tcgcttcatc tcttggagtg gtagagttgt ctattgctct acattatgtg
     1981 tttgatactc ctgtcgatcg tgtaatctgg gatgttggcc atcagtctta tgctcataag
     2041 cttttaacag gtagacgtga tatattttca actcttagac agtttggggg catgagtggt
     2101 ttcccaaaaa tttctgaatc cgaatatgat tgttacaatg tgggacatag tagtacatcc
     2161 ttatcgcttg cggccggaga aatatgtgct cgtgatttaa aaggtgagaa ttatgcagtt
     2221 gttcctgtaa tcggtgatgg ttccatgaca agcggcatgg cttttgaggc acttaatcac
     2281 atagggcata tcaagcgtga tgttatcatc gttttaaatg acaatgaaca ttctatatct
     2341 cctaatgtcg gagccttatc cgaatatttt accgatatca taacaggaaa gttctataat
     2401 cgcactaggc gccagattta tagatttttt cgatcgattc ctcttattgg acgcgctgtg
     2461 gaaagattgg cacaaaccac taagaaattc ataaaagata taattacacc cggaaagctt
     2521 tttgaagatc ttggtattcg ttactttggg cctgttgacg gacataactt gacacattta
     2581 atagaaatat ttaacagggt aaaacagata aataatggcc ctaaaattat acatgtacgt
     2641 acaataaaag gtaaaggatt ttgcccagca gagaattgcc ctgaaaaata ccatggcatt
     2701 gctcctttta acagagaaac cggcaagcct ctgaaactca agacaaggtc actttcagaa
     2761 gtggcaggcc ataaactgtg tgaacttgcc ggtaaagatg attcaattgt agctattact
     2821 gcggcaatga cctctggaac tggtcttgat gaattttcga aaaaatatcc ccggcgtttt
     2881 tttgatgttg gtattgcaga gcagcatgct gtgacattcg cgacggctat ggcaaaaaac
     2941 ggattaaagc ctttttttgc tgtatactca acttttttac agcgttctta tgatcagctt
     3001 gtgcatgacg ctgcattaat gcatctgcct gtttgtctgc tagtagatag agctggtatt
     3061 gttggtgacg atggcgaaac acaccatgga ctatttgatg ttggttttat gcgggggatt
     3121 cctgggctaa gaatatttac tcctgccgat gagacacagc ttgaaaagct gattgaatat
     3181 gtggtcgtta atccggatgg tccttcagca ataaggtatc ctcgtggtgc cagtgataac
     3241 tatccaacaa tggtgacaga ttcttttgat ccatttggtg ttgatatcga taatcgtgga
     3301 gacgacattt taattattac tgccgggttt aaacgaaaat atgctttgga agtttcaaat
     3361 atacttacaa aaaagaatat cggctgcact gttcttaatc ttcatgctat agctccgata
     3421 gccaccggta ttattagcaa aaatattgta cgttgccgga attattgtgt tttagaagat
     3481 cactatatta caggcggttt atcagaagct gttacagcgg ttctggaatc ggaactaaga
     3541 gtgaaatttt tgatgaattt cggatatcca gctgaattta tctgtcatgg cacatcagac
     3601 acaatcgaga acaaatacgg gcttcttcct tctcagattg ccgagtcaat atatcagggt
     3661 tttaatggaa agaatagata aacatcttgc tcaatcaggt ttgagtagta ctcgtgaaca
     3721 ggcaaaaaag gagattcttg ccggatgggt taagataaat ggagagacct gtctggatgt
     3781 ttctcgaaag gtcgaaggaa tagaaaacat tacggtatct cgcccaaaag gtttatttgt
     3841 tagccgtggt ggatataaac ttgaaaaagc tctgaattat tttaatattg atgtaagtgg
     3901 attaactgtg cttgatttgg gcgcttctac tggtggcttt tcagattgca tgttgaagcg
     3961 aggcgcatca tttgtttatg ctgttgatgt ggggtatggc cagcttgatt attctttgcg
     4021 tattgataaa cgcgtggagg tgatggaacg aactaatgcc cgaaatctta caatagaaaa
     4081 ttttgaaaag ggaatcccga cttttgtttc cgctgatctt tcattcatat cttttacaaa
     4141 ggtttttccg gtaataaggg gtctctttaa agatataaaa ggtgttgccc tgataaaacc
     4201 acagtttgaa gcacagcggg gtgaacataa aaaaggggtt gtaaaatctt cttctgttca
     4261 tttagatatt ttaacacggg ttttaacaat tctttcggat caagggtgtt cattgtcagg
     4321 tttaacacat tcaccggtga cagggccgaa gggaaacatc gaatttttgt tatatttttc
     4381 acttgtagag gatagttctt tcgatctttc atcaatagaa gatattgtaa agtgtgcctg
     4441 ggatgagctt aaggaaaagt catgaagatt ataactatta ttgcggttgt catctgtttt
     4501 ggatcgttga tttctgcttc tgaggaatcc attattgctt accctgtacc ttttaatcct
     4561 ggaacaacct cgttaaccat agcagataaa tcatctagtt ttaccggtgt gccaattcgt
     4621 ataaatattg atatttttga tatatgtggc gataaaattt tcaatggatc gtacaattca
     4681 tttccggttc tctggaaggg gtacagttcc ggtggtaagc gagtatctaa cgggttatat
     4741 atagtcaaag ttacagtgga gaatctttca accggatcaa ttgaaaaaaa agtgatcaga
     4801 attttggtaa aaaaatgaag atagcaatat cgttaaaagt atgcttgcga tattttttgt
     4861 gggtaaacgt tcatatatgt tttttgctca ttatttctac attttcgatt tttgcagaca
     4921 gtactggtgt tcgtgccgca ttttccagat taggtatagc tggtgcgcat aaccttgcga
     4981 tggggggcgc cgtagaggct gttaccgatg atgttttttc agtattttgg aatcctgccg
     5041 gtttgactca acttaggagt cggaaaaaaa gtataaggga agatgtccgt acaaaacttc
     5101 agcaaaatca aatagacaaa ataactgagc gtgacttgaa acgactttca gatgatacac
     5161 ctaagttttt ttttcaactt ggtgcggcgg cttcaaaact gcaggaagat cgagaggctg
     5221 cttttgttgc aggtgctttt aatcttggta ataccgttct aggtatggga gtacttggta
     5281 tctattctga taaaattgca acatattcga atgaaggggt ttttactggt gatacatcct
     5341 atcaggctgg agtttcttat ttatcagttg cctatccaac agaatatgtc tctatagggg
     5401 taacggctaa aacgttttac gaaaatattg accgaacaaa ttttgcagga ggagctttcg
     5461 atgcaggcat tcagactgat tttatacctc ttgttcagat aggggttgtt gttcaggata
     5521 ttggtgttgg tcttatgcct attgaaaagg atgatgaagt aaaaaatgag tatgaatttg
     5581 gcaaacccgt ttttcggttt aatatcgctc ttgtctctag aacagcaaac atggttgtat
     5641 ctgcaggcat tattaaaaaa tttgaagaca aaagtagtga ctataaattg ggtatcagat
     5701 actatttgtt gagtaatttt gcttttatgc ttggagtaaa tgaatcttta tttacaactg
     5761 ggacaatgat taattttttt gacgccgatt tttcttacgc agtatcagtt gacaatatcg
     5821 atatgggata taatcataca gtcagttttt cttatctgtt ttaaataagg ggtaccatta
     5881 ttgagctttt tttccgatat taagattatg ggtattgtaa atgttacacc ggactctttt
     5941 tatgatggtg gacgctataa tgatctttct aaagcagttg atcatgcgat gtgccttgta
     6001 agggatggcg ctgatattct ggatataggc ggagagtcaa caaggcctgg agctgcgcct
     6061 gttagcgaaa gcgaagaaat ggaacgggtt ataccgctta tcgagagact ttcagcaatt
     6121 tgcgatgtta ccttgtctgt agatacgaat aaatcttcgg tagccgaagc ggctcttaat
     6181 gccggtgcat cacttgtaaa tgatataagc gcgatgactt ttgataaggg tatggctagg
     6241 gtaatatccg aaaaaaatgc tggggtagtt cttatgcata catctggtag accagatgta
     6301 atgatgcaca aaacagaata taataatctg gtagccgata ttaaatcatt tttactggaa
     6361 agagctaatt atgctcaaaa atgcggtatt gaaaaaaata aaataataat tgatccgggt
     6421 attggttttg gaaaaactat cgagggtaat tataatatat tatcatcact tgatgagttt
     6481 atctcaacag attttcctgt tttaatcgga ctgtcaagaa agtcgttgat cgcaaaacta
     6541 tataaagaag atgaagatag attgccagca acaattgcat tgaatactat ggcgttaagt
     6601 aagggagcgg caattattcg tgtacatgat gtaaaagaac ataaactagc tctcgacgca
     6661 ctaaaaatgc ttcgtttgta aaaggtgtag atattatgga gctctttttt tataaccttg
     6721 tttatatatt gtcaattatt tgggactatt tgcgtaatat cattgatatt gttcttgtcg
     6781 cggttatttt ctatattatt tacacctatc ttgtaaagac aaaggcaatt caactgctta
     6841 gaggattttt atttgtcgcg gtattgtggt tgcttgcaag aatttttcat tttgagacac
     6901 tttattggtt gattaccgcc atgacctcat atttttttat tgctatcatt atactttttc
     6961 agcctgagct tagacgggta ataagcagat ttggtcagag tggatggctt gcaaatattg
     7021 atccagaaga ctcattgcat cttgatgagc ttgttaacgc tgtagcagct atggcggagg
     7081 ctagaatcgg atctcttatc gtttttgaga gggataactc attgcgttca tatgctgatt
     7141 cgggtgttgt gattaatgca ggtattacag aagagcttat ccgtacgata ttttttccga
     7201 atacggaact tcatgacggt gcattgatta ttcaacaggg taaaattgta tctgctgcat
     7261 gttatttgcc gctaagcgat tcacggcagt tgcgaaaaaa tcatggagca cgtcacaggg
     7321 ctggcttagg tatagctgaa gaaacagacg cacttgttgt tttgacctcc gaagagacag
     7381 ggcaaattgc aattatggtt aatagcaaac ttttcccgcg aattaaaaga gaagaattaa
     7441 aaaatattat tcttttcttt atgaatccca aaactgctta tgaagagagg tattcaataa
     7501 aatgatatct caaaaggaaa tgataataga ttttataaaa tcaaaggatt ttatagccaa
     7561 aatagtttgc ctaatactgg ctgtaatttt gtggagttat atcggaaacc aaggtaattc
     7621 tgatgttacg ttaagaatta aggcggagta caagaatctt tcttcggaat atgttgtatc
     7681 agatattcag aaaacacatg ttaatgtaaa aataagcggt agagaagatg aactggatgc
     7741 ctttaataaa aacgatatta gagtctttgt cgattttaaa aatgctgtac ctgataaccg
     7801 ctaccgatac gatgtagaac ttgtgagttc ccctgatatt ccccggggaa tagatataaa
     7861 acttatggac aaaagtattt ttgcaactgt ggagaagaag gtttcgaaaa ctgttttcat
     7921 tgaaccggtt attgccggtc aggtaagatc gggatatttc gcaggtaacg taagagccca
     7981 acctgatcgt gtagttgtat acgggtctcc agaacaggtg caaaaaattg atttaataaa
     8041 aaccatgcca ttatctgttg cacatgcgtc agataatgta tcgaatgaag ttctgcttga
     8101 tacagatgga tacagtttta ctgaaataag tagtgataaa actcttgtaa ctgccgaagt
     8161 ttttagcgcc caaaatgttg tgaagtatga gcgcgaaatt gatctatttg gaaaaatgga
     8221 taattattta tatgagatac atcctgataa aaaaattaca gtatatatta gaagtaaaga
     8281 tggttctgtt ccacaactgg atataaatga cgtaagggta tggacggacc tttcgaatat
     8341 tgactcttcg aaatttataa catctgatgg caatattatc tcttcactta gagtaacaag
     8401 ccgtgttcgt gccgttatca ataatcgtac tgatagtgta cagattatca atatcgtacc
     8461 tgatgttgtt acaatacgta ttaaaaatga aaattgaaag aacaataatt tttatgaggt
     8521 ttgcagcatt atgaaatatc cggttcttgg tgtaaatatt gatcatgttg cgacattaag
     8581 gcaagctagg ggggtagggt accccgatgt tgttgatgct gcaaagattt gtgaggagag
     8641 tgatatattc tgtattactg ctcatttacg tgaagatcgt cgccacatac aagactcgga
     8701 tatttatgag ttaaaaaaga gtattagaac acgattgaat atggagatgg ccatcgctcc
     8761 cgatgttgta aagatagcac tggaagtaaa tccctatatg gttaccatag taccggaaaa
     8821 acgtgaagaa ttaacaaccg agggtgggct caacctgtca attcttaaag atgaccttaa
     8881 aaatgttata gcatcttttg ataaaaagtg atacaataat tcctttttta tcaaaagatg
     8941 cgataacatt ttt
//
LOCUS       DAOTGZ010000105         8936 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_3064
            NODE_9342_length_8936_cov_4.624930, whole genome shotgun sequence.
ACCESSION   DAOTGZ010000105 DAOTGZ010000000
VERSION     DAOTGZ010000105.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868088
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 8936)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8936)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:22:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,785
            CDSs (total)                      :: 2,751
            Genes (coding)                    :: 2,732
            CDSs (with protein)               :: 2,732
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 12 of 19
            Pseudo Genes (internal stop)      :: 5 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8936
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9342_length_8936_cov_4.624930"
                     /isolate="AMR_MDS_3064"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            58..453
                     /locus_tag="P5123_08005"
     CDS             58..453
                     /locus_tag="P5123_08005"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRX16241.1"
                     /translation="MLLLLKQKLHLWKREKNETVQTIALEDCANVWFHHFASLSLRES
                     GDKHGTFSGCSPETVQIIAREDCAIIWFHHFASLSLRESGDMRGAFSGCSPETVQTIA
                     LKDCAIIWFHHFASLALRESGDMHGTFSG"
     gene            810..1793
                     /locus_tag="P5123_08010"
     CDS             810..1793
                     /locus_tag="P5123_08010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007221558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HRX16242.1"
                     /translation="MDYIEIEKLTELAKKENSAVQQLRDEIRKVIVGQDYLIDRIIIA
                     FLCDGHILLEGLPGLAKTLAVRAFAKTIDSDFARIQFTPDLLPADILGTQIYNPKTME
                     FSTKKGPIFSNIVLADEINRAPAKVQSALLEAMEERQVTIGDESFPFSVPFMVLATEN
                     PIEQEGTYPLPEAQLDRFFMKCLVHYPTEEEEFQVLNRYSSISVDTIKPVISSAELVK
                     KREFIQSIYIDEKLLRYIVSIVNFTRKPTNPDVARFLEFGASPRASIALMKASRCVAF
                     INGRGFVTPEDIKEIGYDVLRHRIILSYEAEAEDVTNEDVCRMIFDSVEVP"
     gene            1793..2617
                     /locus_tag="P5123_08015"
     CDS             1793..2617
                     /locus_tag="P5123_08015"
                     /inference="COORDINATES: protein motif:HMM:NF012321.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF58 domain-containing protein"
                     /protein_id="HRX16243.1"
                     /translation="MTSSSYLKKIRFSISKKINTAFIGQYRSAFRGNGLLFDAIREYQ
                     PGDDVKNIDWKVSARMRNLFVKQYQEERELNIVIMIDMSGSMDFGSTRTKKDVLMEFT
                     ALLLFLAEYTNDRVSVLLFTDEVEWFFRAKRCRRYVLKVLNDIIHFKPKNKKTGINEA
                     LDFLSKVTRKRSVVFMISDFIDNAYDTQLKRVVRKHDFIPVSVSDPQESSLSFFGLAS
                     FYDLETDKVIYATETSYNDVNLKQYGVEPLKLSTDSSVEHDVLEYFKKRNKKRLHG"
     gene            2620..3516
                     /locus_tag="P5123_08020"
     CDS             2620..3516
                     /locus_tag="P5123_08020"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRX16244.1"
                     /translation="MKKILLIIIIFTSISLSAQINVSTSVQRSSTNVGIENSYTVEIT
                     SESPDFKVKNPESTFYTEESETVPLYMITDTGVKDYNEKGVFIRTITYKIVYFITGTY
                     DLPRPVVHDSEDNPVNFTAPRIEVAPVSKEDSFADIEPPFARSGNYKRIILLILALLA
                     LAVIIYFIVRIVKKRINKPKAIPVIVPVEKFMESYNKLQQKQLLENDQIEDYCTELSI
                     IFRRFLDDRFKMGALDLTTDEIFRACSSVNIDSTNANRLKHLMYLWDLAKFAEMRPSR
                     EIVEGNANETLDLVKDLDRGGQ"
     gene            3516..4505
                     /locus_tag="P5123_08025"
     CDS             3516..4505
                     /locus_tag="P5123_08025"
                     /inference="COORDINATES: protein motif:HMM:NF012321.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HRX16245.1"
                     /translation="MDFQSPNYLYLFIPFAIFTLWYWIKRLNIREHSLPLSSADIVSK
                     KRNFRALLYPYIPVLRFLSVALIIFASARPGNDISYSEVSSYGVDIMIVTDVSLSMLG
                     TDFQPGNRLEVSKNLLKSFVSDRTTDRVGMIVFAGEAYVQSPMTTDYNIFNELIDEVD
                     FDSVSEEGTAIGNAIALAVARMSDSDTASKIMVLITDGSNNSGIIEPDTAAQIAAEKG
                     IKIYTVGIGTKGDYTIIIPRGPKKGRYRASGDYDEEGLKKISELTDGKFFRATTETEF
                     MEYIEEINRLEKSKFTVKQYHEFDGKWMKYLLLAAFIFIFEQLIRIVVFRKVP"
     gene            4512..5513
                     /locus_tag="P5123_08030"
     CDS             4512..5513
                     /locus_tag="P5123_08030"
                     /inference="COORDINATES: protein motif:HMM:NF012321.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HRX16246.1"
                     /translation="MSFKNIDYLLWIIISGTLLSLFAMAFMFYRFYVLKTFFSRVSNI
                     RFFKISYFIRSTVIILIIALFAIVVLRPQWGFHMREKNVKGTDVLVALDVSRSMNSRD
                     VAADRLSRAKQAIRYIVESVPGRSGLIVFAGEAFLQCPLTTDKAAFSMFLDYTDTNSV
                     ALQGTDIGKALDEAERVFEKKQLTNKVLILLSDGEDHEKSLGPVLSRLQDNEVTVYTV
                     GIGTDAGDLVPVVDSDNAGVYLKDSSGNIVKSVPDRKTLKDIASRTGGSFTDISASFS
                     GLKRVIDALNSQTASDQGSRFVREPNDQFQYFAVMLVILMIVELLLNVCWKKQNKRG"
     gene            5519..6265
                     /locus_tag="P5123_08035"
     CDS             5519..6265
                     /locus_tag="P5123_08035"
                     /inference="COORDINATES: protein motif:HMM:NF024824.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HRX16247.1"
                     /translation="MKNKYLKKFIYNLRPLVIGISLLQLALLDPYFDSMDEGNRLYND
                     NKYTDADEAYNRALEYMPSKKEKPYVNFNRGTAQFKAGNYEGAVDYFKKALESSDADL
                     QKRAFYNLGNSYYKKGDIKSALEHYMNALKIDPEYQKPKQNIEAILKEQNQQDKQDQQ
                     NDQKKENSDQENQNDQKPDESEQKGDESSDRDKGEKNKRDMDGHKKGDHIEKKLSKEQ
                     IDNILNHFKQRPVQKGKGDGRAGRALEKNW"
     gene            6234..7922
                     /locus_tag="P5123_08040"
     CDS             6234..7922
                     /locus_tag="P5123_08040"
                     /inference="COORDINATES: protein motif:HMM:NF024973.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BatD family protein"
                     /protein_id="HRX16248.1"
                     /translation="MLEELLKKTGKFVFILFSYGLLYGASIETSLQRPEIYEDEATIL
                     EIAVEGERNASITNIPSSKDIEIQPAGQSRSFSFGTGGSSSSLIFRFRIVPLSNGVFT
                     IPSITVETGDGVYESEPLKLTVLKGSNPAAKNSAPVRDPFDMFFEDLREPERRKPLIM
                     AEKSISRDIVYAGESVILRYYIAAENDIDVLPEAVFNNRWDGFILKPFDENLPQTPYN
                     FSGRNLKRYHVVSYRLVPTVSGNYNLTNDNISFIYSDGAARFSPRMRGSLPFNAEELD
                     VLPLPLEGRPQEFSGNIGRFDIDLVSDKNETFVGNEAIIIFSVSGRGEMSQLSPPTIN
                     DNAAFRIVFDSRDDKYRFVNGQEEGSVNFKYILIPNSEGNYTLDEIAFNFFDPDSGQY
                     TELKTDPLEFVVKPAPAMIASGDEQNQVIENKPGILMYIGIAILILCVIASLVFVFYR
                     ELSRNRIHRGEKPKKKPGLNENPIAPLPDVKELAGLYIMRIDNAFSGKDSSLCFKELL
                     TAVNFILREKDLPAEGMADVTKIRDYMVSSRYGGGELDFHLLLDIKKKIVAYLK"
     gene            complement(7914..8696)
                     /gene="mazG"
                     /locus_tag="P5123_08045"
     CDS             complement(7914..8696)
                     /gene="mazG"
                     /locus_tag="P5123_08045"
                     /EC_number="3.6.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020384341.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside triphosphate pyrophosphohydrolase"
                     /protein_id="HRX16249.1"
                     /translation="MKFNEQRELYRLEELADTLHGENGCPWDRKQTHQSLKKYLIEET
                     YEVIDAIDSGSPDKLKEELGDLLYQVYAHCRVSNSFKIDDVAKMIIQKLITRHPHVFG
                     EEVADTPDEVADKWERIKKKERTDKKILEGVPAHLPALLKAYRVQEKVSRVGFDFPDI
                     SDTVMKLEEELEELKEAVKNGNRAEVEDEFGDILFSIVNIARFASIDPEQSLRNAVAK
                     FTSRFNEVEDMVDKSGKSFDDYTMEQLDKMWEKAKKHHPTSF"
BASE COUNT         2654 a         1651 c         1854 g         2777 t
ORIGIN      
        1 ccttgtggcg ttttctttgg tcctttcttt ttgccataaa aagaaaggac agaaaatttg
       61 ttgcttttgt taaagcaaaa actacatctc tggaagagag agaagaacga aaccgtccaa
      121 acaatcgcgc ttgaggattg tgcaaatgtt tggtttcacc acttcgcgtc gctgtcactc
      181 cgcgagagtg gtgataagca tggaacattt tcgggctgtt cgcccgaaac cgtccaaata
      241 atcgcgcgtg aagattgcgc aattatttgg tttcaccact tcgcgtcgtt gtcactccgc
      301 gaaagtggtg atatgcgtgg tgcattttcg ggctgttcgc ccgaaaccgt ccaaacaatc
      361 gcgcttaagg attgcgcaat tatttggttt caccacttcg cgtcgctggc actccgcgaa
      421 agtggtgata tgcatggaac attttcgggc tgatcgcccg aatccgtaaa aacaatcgcg
      481 cttgaagact gcgcaactgt tttgactatg tggtatattc tttttgccaa ttttttttaa
      541 aaaaatattt tttattccct ggttgctttg taacaaaatg agggtagaaa gctcttttca
      601 gaatatgatt aaaaataccg gtaatttcga aatagcaatt tactttttac tgtttcgtaa
      661 ccggattttt tttgttgtgc agctgtttgc acttgacaca tactatccga ttgttttaaa
      721 aaatacgcgt tcgcggtttt gttttaaaca gtaatattaa ttggttttta taaatgcaat
      781 aaatcattta tgatttttag gagttagtta tggattatat cgagattgaa aagcttacgg
      841 agcttgcaaa aaaagagaac tcggcggttc agcagttgcg tgacgagata cgtaaggtca
      901 ttgtaggtca agactactta attgaccgta taataattgc ctttttgtgt gatggccata
      961 tacttttaga agggcttccc ggtttggcaa aaactttggc cgtgcgagcg tttgccaaaa
     1021 caatagattc agactttgcg cgaattcagt tcactccgga tcttttacca gcggatattc
     1081 tgggcaccca gatatacaat ccaaagacaa tggaattttc cactaagaag ggaccaatat
     1141 tttcgaacat agtgcttgcc gatgagataa accgtgctcc ggcaaaggtt cagtctgcac
     1201 ttctggaggc aatggaagaa cgtcaggtaa ctattggtga tgaatctttc ccgttctcgg
     1261 ttccgtttat ggttcttgca acagagaacc ctattgaaca ggagggtaca tatccgcttc
     1321 ccgaggcaca gcttgaccgt ttttttatga agtgtctggt gcactatcca acagaagagg
     1381 aggagtttca ggtgcttaat cgctattcct ctatctcggt tgataccatt aagccggtta
     1441 taagcagtgc cgagcttgta aagaaacgcg aatttatcca atccatatat attgacgaga
     1501 agttgcttcg ttatatagta tcaattgtaa actttacaag aaaacccact aatcccgatg
     1561 tggcacgttt tctagagttt ggagcaagcc caagggcatc aatagcgcta atgaaagctt
     1621 ctcgctgtgt tgcatttatc aatggtcgtg gatttgttac tccagaagat attaaagaga
     1681 ttggttacga tgttctgcga catcgcataa ttctttctta cgaggccgaa gccgaagatg
     1741 taacgaatga agatgtatgt cgaatgatat ttgacagtgt tgaggttccg taatgacttc
     1801 gtcgtcttat ctgaaaaaaa tcaggttttc gatatccaaa aagattaata ctgcttttat
     1861 cggccagtat cgttcggctt ttcgtggtaa cgggctttta ttcgatgcta ttcgtgaata
     1921 ccagccgggt gatgatgtca aaaatatcga ctggaaggtt tcagcacgaa tgcgtaatct
     1981 ttttgtaaag cagtatcagg aagagcgcga actaaatata gtaataatga tcgatatgtc
     2041 gggatcaatg gatttcggtt ctacccgcac taaaaaagat gtccttatgg agtttaccgc
     2101 tctgcttttg tttttggccg aatataccaa tgatcgagta tcagtacttc tttttactga
     2161 tgaggtcgag tggtttttcc gcgccaagag gtgcagacgt tatgtgctaa aagtgttaaa
     2221 cgacattatt cactttaagc ccaaaaacaa aaaaacaggc ataaacgagg cccttgactt
     2281 tttgtcaaaa gttacacgaa aaagaagtgt tgtttttatg atttcagatt ttatcgataa
     2341 tgcctatgat acccagctta aaagggttgt tcgaaagcat gactttattc cggtttctgt
     2401 ctctgatccg caagagtcaa gtctttcgtt tttcggcctt gcgtcttttt atgatctgga
     2461 aaccgataag gtaatttatg ctaccgaaac atcttataat gatgtgaatc taaaacagta
     2521 tggagtggaa ccgctgaagc tttcgaccga ctcttcggtt gaacacgatg ttcttgaata
     2581 ttttaaaaaa cgtaacaaaa aacgcctgca tggttaacta tgaaaaaaat tttacttata
     2641 ataataattt ttacttctat ttctttatca gcgcagataa atgtatcgac ctctgtacag
     2701 agaagtagta caaatgttgg catcgaaaac agttatactg tcgaaataac ctcggaatct
     2761 cccgatttta aggttaaaaa ccccgaaagc accttctata ccgaagagag cgagactgtg
     2821 ccgctttaca tgattactga tacaggtgta aaggattaca atgaaaaagg tgtttttatt
     2881 aggacaatta cctataaaat tgtctatttc attacaggta catacgatct gcctcgtcct
     2941 gttgtccatg atagtgaaga taatcctgta aactttacag ctccgcgtat cgaggttgct
     3001 cctgtatcta aggaagactc ttttgccgat atagaaccac ctttcgcaag gtctggaaac
     3061 tataaacgaa tcattctttt gattcttgcc ttgttggctt tggctgtgat catctatttt
     3121 attgttcgta tagtaaaaaa acgaataaac aaaccaaagg caataccagt aattgttccg
     3181 gtcgaaaaat ttatggaatc ttataacaag cttcaacaaa aacaactact tgaaaatgat
     3241 caaattgaag attactgtac cgagctttcg ataattttca gaagatttct tgatgatcgt
     3301 ttcaagatgg gagctcttga tcttacaact gacgagattt tcagagcttg ttcttcagtg
     3361 aatattgata gcacaaacgc gaaccggtta aaacatctga tgtatctttg ggatctcgcg
     3421 aagttcgcgg aaatgcgccc ttcacgcgaa atagtcgaag gtaatgcaaa cgaaactctt
     3481 gatcttgtaa aagatcttga tcggggaggg cagtaatgga ttttcaatcc cccaattacc
     3541 tgtatctgtt tattccgttt gctatattca ctttatggta ttggataaaa agattaaata
     3601 ttcgcgaaca ctctttgcct ctttcgtccg cggatattgt ttcaaaaaaa agaaatttta
     3661 gagcattact ttatccgtat attcctgtac ttaggttttt atctgttgct cttattattt
     3721 ttgcatcggc acgacctgga aacgatataa gttacagtga agtcagttct tatggtgtag
     3781 atatcatgat cgttacggat gtctcgctat caatgttggg taccgacttt caaccgggta
     3841 atcgccttga ggtatcaaag aatttattaa aaagttttgt gtcagataga acaacagatc
     3901 gggtgggtat gatcgtgttt gccggtgaag cttatgtaca gtccccaatg acaaccgatt
     3961 ataatatttt taacgagctg attgacgaag tagacttcga ttcggtttca gaagagggaa
     4021 ctgctattgg taacgccata gctcttgctg ttgcccgaat gagtgattct gacaccgcaa
     4081 gtaaaatcat ggtattaatt accgatggtt ctaataatag cggaataatc gaaccggata
     4141 cagcagctca aattgcagcc gaaaagggta tcaaaatcta tactgttgga ataggcacta
     4201 aaggtgatta tactattata atacctagag gtccaaagaa aggacgttac cgtgcctctg
     4261 gtgattatga tgaggagggc ttgaaaaaaa tctccgaatt aactgatggc aaatttttta
     4321 gagcaacaac cgaaaccgaa tttatggaat atattgaaga gattaaccgt cttgagaagt
     4381 caaaatttac tgttaagcag tatcatgaat ttgacggtaa gtggatgaag tatctgctat
     4441 tggccgcgtt catttttata tttgagcaat tgatacgtat agttgttttc agaaaggttc
     4501 cgtgaggtac tatgagtttt aaaaatattg attatctttt gtggattata atttctggaa
     4561 cgttgctatc ccttttcgca atggcgttta tgttttaccg tttttacgta ctaaaaacat
     4621 tcttttccag agtatcgaat ataagatttt tcaaaatcag ctattttata cgttctacag
     4681 ttattatcct gataatagcc ctctttgcga ttgtcgtatt aaggccccag tgggggtttc
     4741 atatgcgcga aaaaaatgta aaaggcaccg atgtgcttgt agcgcttgat gtaagccgaa
     4801 gcatgaacag ccgagatgtt gctgctgaca gattatcccg ggcgaagcag gccataaggt
     4861 atatcgttga atcggtaccg ggaagatccg gtttgatagt ctttgccggt gaagctttct
     4921 tgcaatgtcc tttgactacc gacaaagctg cattttcaat gtttcttgat tataccgata
     4981 caaattccgt tgcccttcag ggtactgata ttggcaaggc tcttgatgaa gcagaaaggg
     5041 tttttgaaaa aaagcagtta accaataaag tacttatttt gttatcagat ggagaggatc
     5101 atgaaaagag tcttggccct gttctttcac gattacagga taatgaagta acggtgtaca
     5161 ctgttggtat tggaaccgat gccggtgacc ttgtgcctgt agttgattct gataacgcgg
     5221 gtgtgtacct gaaagacagc agtggaaaca ttgtaaagag tgttcctgac cgtaaaacct
     5281 taaaggatat tgcttcacgt actggtggat cgtttaccga tatttctgct tcttttagtg
     5341 gattaaaaag agtcatagac gcgttaaatt ctcaaaccgc tagtgatcaa ggttctcgct
     5401 ttgtaagaga gccgaatgat cagtttcaat attttgccgt aatgcttgtt attctgatga
     5461 ttgtcgagct actgctcaat gtgtgttgga aaaaacagaa taaaagagga tagcatttgt
     5521 gaaaaataaa tatttaaaaa aatttatcta taatctccgt cctcttgtaa tagggatatc
     5581 cctgttacaa ctcgctcttc ttgacccata ttttgattcg atggatgaag gtaatcgttt
     5641 atataacgat aacaaatata ccgacgcgga cgaagcctat aaccgcgcac tcgaatatat
     5701 gccatctaaa aaagagaagc catatgtcaa ttttaatcgc ggtactgcac agtttaaagc
     5761 aggtaactac gagggtgctg tcgattattt taaaaaagct cttgaaagtt ccgatgctga
     5821 tcttcagaaa agggcttttt ataatctggg taatagctat tataaaaaag gtgatatcaa
     5881 aagtgctctg gaacattaca tgaacgccct aaaaattgat cccgagtatc aaaagccaaa
     5941 acagaatatt gaggcaatat taaaagaaca aaatcaacaa gataaacagg atcagcagaa
     6001 cgatcagaaa aaggaaaaca gcgatcagga aaaccaaaac gaccaaaaac cagatgagtc
     6061 cgagcaaaag ggcgatgaat catcagatcg cgacaagggt gaaaaaaaca agcgtgacat
     6121 ggatggccat aaaaagggag atcatataga gaaaaaactt tcgaaagaac agattgacaa
     6181 catactaaat cattttaaac agagacctgt tcaaaaaggt aaaggggatg gccgtgctgg
     6241 aagagctctt gaaaaaaact ggtaagtttg tctttattct tttctcttat ggtttactct
     6301 atggtgccag tattgaaact tcacttcaac ggccagagat atacgaggat gaagctacta
     6361 tcttagagat agcagtcgaa ggcgagcgaa atgcttccat tacaaatatt ccctcaagca
     6421 aggatattga aattcagcct gccgggcaga gccgttcttt cagttttgga actggtggca
     6481 gcagttcttc actgattttt cgtttcagaa ttgttcccct atcaaacgga gtatttacca
     6541 ttccatccat cacagtcgaa acaggtgacg gtgtttatga atcagaaccg cttaaactta
     6601 cagttttaaa aggttcaaat ccggctgcaa aaaattcggc gcctgttcgt gatcctttcg
     6661 atatgttttt tgaagatctg cgtgaacccg aacgccgcaa accacttatt atggccgaaa
     6721 aaagcatctc tcgtgatatc gtttatgcag gagaatccgt tatacttcgt tactatatcg
     6781 ctgccgaaaa cgatattgat gttttaccgg aggcagtttt taataatcga tgggatggtt
     6841 ttattttaaa accttttgat gagaacttgc cccaaacacc gtataatttt tcgggacgaa
     6901 atttaaaaag atatcatgtt gttagttacc gattagtacc caccgtatcc ggaaattata
     6961 atctaacaaa tgataatatt tcatttattt acagtgatgg tgccgcccga ttttctccaa
     7021 gaatgcgtgg gtcattaccg tttaatgccg aagaacttga cgttttgcct ttgccgctag
     7081 aagggcggcc gcaagagttt tctggtaata ttggaaggtt cgatattgat ctggtttctg
     7141 ataaaaatga gacctttgtt ggcaatgagg ctattataat ttttagcgta agtggccggg
     7201 gtgaaatgtc acaactatcc cctccaacaa taaatgataa cgctgcattc cgtatcgtat
     7261 ttgactcacg agacgataaa tatcggtttg ttaacgggca agaagagggc agtgtcaatt
     7321 ttaaatatat attgatacct aacagtgagg gtaattatac ccttgatgaa attgcgttca
     7381 atttttttga tcctgatagt ggacaatata cagaacttaa aaccgaccca ctcgagtttg
     7441 tggtcaaacc tgcaccagct atgatagctt ctggtgatga gcagaatcaa gtaatagaga
     7501 acaaacccgg tatattaatg tatattggta tagctattct gatcctttgt gtaatcgcat
     7561 cacttgtctt tgtgttttat cgtgagttat cgcgaaacag gattcaccgt ggagaaaaac
     7621 cgaaaaaaaa accaggactc aatgaaaacc ctatagctcc attaccggat gtaaaagagt
     7681 tggccggtct ttatattatg cgaatcgata atgcgttttc tggcaaagac tcctctcttt
     7741 gttttaaaga gcttcttact gctgtaaatt ttattttgcg tgaaaaagat ctgccagctg
     7801 aaggcatggc agatgtgaca aagatacgtg attacatggt tagcagcaga tatggcggtg
     7861 gagaacttga ttttcatcta ctcttagaca taaaaaagaa aatcgtagcc tatttaaaat
     7921 gatgtagggt ggtgcttttt tgctttttcc cacattttgt ctaattgctc catagtataa
     7981 tcatcgaagc tctttcccga tttatctacc atatcttcga cttcattaaa tcgagatgta
     8041 aatttagcaa cggcgtttct taatgactgt tcaggatcaa tagaggcgaa tcttgctatg
     8101 ttaactattg aaaagagtat gtcgccgaat tcatcctcaa cttcggcacg gtttccattt
     8161 tttactgcct ccttgagttc ttcaagctct tcttcaagtt tcataacagt atcagaaata
     8221 tcggggaaat cgaaaccgac acgagacacc ttttcttgta ccctgtatgc cttaagtaac
     8281 gccggcaaat gagcgggaac tccctcgagt atcttcttgt ccgtgcgctc ttttttcttt
     8341 atccgctccc acttgtcggc aacttcatcc ggggtatctg caacctcttc tccaaacaca
     8401 tgcgggtggc gtgtaataag tttttgtata atcattttgg caacatcatc aattttaaaa
     8461 gagttactca ctctgcaatg agcgtaaacc tggtataaaa ggtctccaag ctcctctttt
     8521 aatttgtcgg gtgatccgct gtctatagcg tctattacct cgtatgtctc ttcaatcagg
     8581 tatttcttaa ggctctggtg tgtctgtttg cgatcccagg gacagccgtt ttcaccgtga
     8641 agtgtgtcgg caagttcttc aagacggtaa agttctcttt gttcattaaa tttcataaga
     8701 ttctccggaa atatccatac aataatagtt tatagttctt tgactttctg tctgtataac
     8761 taatcttaaa acggcaggtg ttaaagcaat taaaaaatca tgttaaatga ttgcaagctt
     8821 aattgaggaa gatcatcttt tactgaatat tcgttattgc aagcttctct ttttttgtaa
     8881 aagaagaaaa tacaagatca taagagtggt caattagctc tttaagcagt ttgtca
//
LOCUS       DAOTGZ010000106         8914 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_3064
            NODE_9369_length_8914_cov_5.193588, whole genome shotgun sequence.
ACCESSION   DAOTGZ010000106 DAOTGZ010000000
VERSION     DAOTGZ010000106.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868088
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 8914)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8914)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:22:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,785
            CDSs (total)                      :: 2,751
            Genes (coding)                    :: 2,732
            CDSs (with protein)               :: 2,732
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 12 of 19
            Pseudo Genes (internal stop)      :: 5 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8914
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9369_length_8914_cov_5.193588"
                     /isolate="AMR_MDS_3064"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..915)
                     /gene="proS"
                     /locus_tag="P5123_08050"
     CDS             complement(<1..915)
                     /gene="proS"
                     /locus_tag="P5123_08050"
                     /EC_number="6.1.1.15"
                     /inference="COORDINATES: protein motif:HMM:TIGR00409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004827 - proline-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006433 - prolyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="proline--tRNA ligase"
                     /protein_id="HRX16250.1"
                     /translation="MRLSQYILPTQKEDPSDAQVSSHRLMIRAGLIRKEAAGMYAYLP
                     AGLRALRKVTAIVKEEMDKAGAMECLMPELTSADLWKESGRWESMGPEMFRIRDRNSM
                     EYALGPTHEEAFTALVRSVVSSYKDLPVNVYQINTKFRDEIRPRFGVIRSKEFIMKDA
                     YSFDVDQNGLDLSYDKMASAYRAIFDRCGLETIPVQADSGTMGGSASEEFMVASEVGE
                     ETLLICRKCGYSANQEKAGFKREDNVAGEVAELKEVHTPNIKTIDDLAAFFKCEKKVF
                     LKTIVFVADGEPVVAVVTGDRDINETKLK"
     gene            complement(937..1962)
                     /locus_tag="P5123_08055"
     CDS             complement(937..1962)
                     /locus_tag="P5123_08055"
                     /EC_number="2.6.1.42"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006928651.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004084 - branched-chain-amino-acid
                     transaminase activity [Evidence IEA];
                     GO_process: GO:0009081 - branched-chain amino acid
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid aminotransferase"
                     /protein_id="HRX16251.1"
                     /translation="MSVVKADLDWSNLPFMYVKTDKNVRYHYKNGSWGAPEICEEDML
                     SINIAAPALHYGQQGFEGLKAFETRDGRVVCFRPDENAKRMNRTNSRINMPEIPEEIF
                     ISAVETVIRENIRFVPPYGTGASLYLRPLMIGVGPKVGLGPADEYIFLVFATPVGPYY
                     KGGFKPVDAIIVEGFDRAAPHGVGDCKVGGNYAAGLPGSKFVKEQGCAVGLYLDPQHN
                     RYIDEFSTSNFIGIKGSSYITPKSPSILPSITNKSLATIAQDMGMLVESRQVEISEVE
                     GFDEVGAVGTAAVITPVHSIKYGDKILTFGEKDSAGPVLTKLYETLTKIQTGEIEDKH
                     RWLLEIL"
     gene            complement(2088..2399)
                     /locus_tag="P5123_08060"
     CDS             complement(2088..2399)
                     /locus_tag="P5123_08060"
                     /inference="COORDINATES: protein motif:HMM:NF018894.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0035438 - cyclic-di-GMP binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PilZ domain-containing protein"
                     /protein_id="HRX16252.1"
                     /translation="MLNKERRAGDRHDCNGDYFFSPDSRNQKYSCLLKNVSVTGACIL
                     TEFPLKNDDQITLHVCLGKDIELKSSVVWKKNDLYGLLFELETPEAFDNISYLINNRP
                     V"
     gene            complement(2454..3197)
                     /locus_tag="P5123_08065"
     CDS             complement(2454..3197)
                     /locus_tag="P5123_08065"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HRX16253.1"
                     /translation="MIHVKNVAVSFDEIKVLDNISFDVPEKGNLVVLGKNGSGKSVLL
                     KSIAGLISINEGDIQIDNVKVGPALYSNGSNERSIVGYVFQKGGLFDSMTVAENIAFG
                     MLRKKYDKSVIDNKIKIVLESVGLSGAEEKYPSELSGGMQKRVSLARCLCLEPKIMLY
                     DDPAAGLDPVLTDSVADLILGIRESAAITSVTVTHDLKFARKIADTVILLYGGKIACM
                     LEVEDFFSKNDPYAKQFIEGSDVGPIDLF"
     gene            complement(3194..4147)
                     /gene="rsgA"
                     /locus_tag="P5123_08070"
     CDS             complement(3194..4147)
                     /gene="rsgA"
                     /locus_tag="P5123_08070"
                     /inference="COORDINATES: protein motif:HMM:TIGR00157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome small subunit-dependent GTPase A"
                     /protein_id="HRX16254.1"
                     /translation="MSNSSQENLGTGIINKVFGLYYTVNDGQKDIACFLKGKIRINKE
                     FKKFTNPVAVGDNVKFQYNDDGSGSIIDLLPRSNLFSRRDKGRNAREDVIAANLDVVL
                     VIQSFLDPPVNPRFVDRLAVRAIKENIEPVLCMNKADLATEEMTNTVIEYYKNSGLKI
                     IITSALNMQGIDSLKKMLTGKRTLLAGYSGVGKSSLINSIYQDLNLAVSDISDKTGKG
                     RHTTTNVIMYKQKDGTELIDSPGVREFGLVDIEPEELGNFFYEFTQYRSDCTFSNCTH
                     EHEPGCGVKDRVESGIVCRERYVSYLNMLESLLEYREQKYR"
     gene            complement(4712..5050)
                     /locus_tag="P5123_08075"
     CDS             complement(4712..5050)
                     /locus_tag="P5123_08075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007463793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NifB/NifX family molybdenum-iron cluster-binding
                     protein"
                     /protein_id="HRX16255.1"
                     /translation="MKIAIPCVEDKLSLHFGHCAYFSFVTFDDTTKEITDEKREDAPP
                     HEPGLLPKWVKEREADMVICGGMGQRAQQLFISQGIKVMVGAPSETPKQIVQSYLDGT
                     LVTGANGCDH"
     gene            complement(5118..5942)
                     /locus_tag="P5123_08080"
     CDS             complement(5118..5942)
                     /locus_tag="P5123_08080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010942056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Mrp/NBP35 family ATP-binding protein"
                     /protein_id="HRX16256.1"
                     /translation="MIDEKLKENMSGVDHKIIVMSGKGGVGKSTVAVNLALSLAQKGF
                     KTGLLDVDLHGPSVPTLLGMEGEHHTQDESGKIRPIDYNENLKAVSIGFLIDSRSDAM
                     IWRGPMKHNVIKQFLTDVSWGDLDFLIIDSPPGTGDEVLAVCQLIENPDGAVIVTTPQ
                     DLALVDVAKSVTFCEKLNIPRLGVIENMSGFWCPHCGKVSDLFKRGGGKKISEISSIP
                     FLGEIPISLVVSDGGDSGKPFSISGDSSDEAAVGFKNAVDKLMTTHFADIRFNAVN"
     gene            complement(5944..6816)
                     /locus_tag="P5123_08085"
     CDS             complement(5944..6816)
                     /locus_tag="P5123_08085"
                     /inference="COORDINATES: protein motif:HMM:NF022077.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HRX16257.1"
                     /translation="MVDTIAVISGKGGTGKSSLTRAFFELEKNAYAADCDVDGANLFV
                     SLDHDIVESEDFIAGFDYEIDAEKCVHCSKCEKLCRFGAIKDFKIDSFLCEGCNVCYY
                     VCGSEAVRKSPKNCGMIHRAKSNAGFFVYGRLIAGEENSGKMVSRVREIAKKHAEEIE
                     SSMLIIDGPPGVGCPVIASVTATTGVVIVTEPTLSGLSDLKRAAGLVTGMNIPVTIVI
                     NRADINIEICEQIEKFAADNDLSFAGRINYSTIFNESQRDRRSVVSYEGSSAADEVKK
                     VWEKIRKDLLKQES"
     gene            complement(6809..7666)
                     /locus_tag="P5123_08090"
     CDS             complement(6809..7666)
                     /locus_tag="P5123_08090"
                     /inference="COORDINATES: protein motif:HMM:NF013793.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HRX16258.1"
                     /translation="MRIVVVSGKGGAGKTTFSVILSLLLSNEVDIELIDSDVEEPNSN
                     LFLGVNLESKSVYRKIPRLNSEICTGCGKCADICAYNAVVMVKQKPLIFTDLCHSCGG
                     CVEVCPVDALTEVDDKYGEIGSGNSENIEFKEGRIDVGKIQSPMLIKELLADKSSDRV
                     QIVDAPPGTACNTVQALYGADFALIVAEPTPFGTSDFKLIHELTKVMNIDSAVVVNKD
                     EGYSENFYKLCKSLDVPVLATVPFDEKLSRYYSIGDLKGYLKNEDISHYKGAVDGLKS
                     LIMSEKENG"
     gene            complement(7799..8623)
                     /locus_tag="P5123_08095"
     CDS             complement(7799..8623)
                     /locus_tag="P5123_08095"
                     /inference="COORDINATES: protein motif:HMM:NF012955.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HRX16259.1"
                     /translation="MKISVILDNCTYRNGLLAEHGLSYLLEGQKSLILDAGATNAASL
                     NYKKMKNAGVSINALVLSHCHYDHTGGLIPLINAIGESQSNPTVVGSNRLFREAFSSS
                     TGEYRSIGMKHSKMEYEKNGINFNLIEEPLVVGECTVLPLGKSVPGALNNHKLWVKDG
                     EEYKPDYFEDESVLLVNGDLGYSVVTSCAHKGLGSILTYVSEQFSIRRFHTVMGGFHL
                     KGASIEQIESCAAAVNHFGVSRLICGHCTGVDGYHDLKSRVTADCIYGAAGDSFVL"
     gene            complement(8623..>8914)
                     /locus_tag="P5123_08100"
     CDS             complement(8623..>8914)
                     /locus_tag="P5123_08100"
                     /inference="COORDINATES: protein motif:HMM:NF014620.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="NifB/NifX family molybdenum-iron cluster-binding
                     protein"
                     /protein_id="HRX16260.1"
                     /translation="KGFMIFDEDSGNWSYLGNSQNYNAPQGAGIQAAGTVLDAGVSVL
                     ISGHVGPKAFSVLKAADVTMYTGAEGTVENALQMYRDGKLVLASEADVEGHW"
BASE COUNT         2673 a         2144 c         1382 g         2715 t
ORIGIN      
        1 ttttaacttt gtttcgttaa tgtcgcgatc tccggttaca accgcaacaa caggttctcc
       61 atcagcaaca aaaactatgg ttttcaaaaa tacttttttt tcacatttaa aaaaagcagc
      121 caaatcatct attgtcttaa tattcggagt gtggacttcc tttagctctg caacttcacc
      181 tgcaacatta tcttcacgtt taaaaccggc cttttcctga ttcgcactat atccacattt
      241 tcgacatatc aatagagtct cttcgccaac ttctgaagcg accatgaact cttccgaagc
      301 cgaaccgccc attgttccgc tatcagcctg aacaggaatt gtctcaagcc cacaacggtc
      361 aaatatggct ctataagccg atgccatctt atcataagac agatctaatc cattttgatc
      421 tacatcaaaa gaataagcat ctttcattat aaactcttta ctacgtataa ctccgaagcg
      481 gggacgtatc tcgtcacgaa acttggtatt tatctgataa acattaacag gcaaatcttt
      541 atacgaagaa acaaccgacc gtaccagcgc cgtaaaagcc tcctcgtgtg tgggtcccaa
      601 ggcgtattcc attgaattgc gatctcttat acgaaacatc tcaggtccca tgctttccca
      661 acgcccagac tctttccata aatctgcact tgtaagctcg ggcattaaac actccatcgc
      721 tccggcctta tccatctctt ctttaacaat tgctgtaacc ttgcgcaatg cccttaaccc
      781 agcaggaaga tacgcgtaca tacctgctgc ttctttgcga ataagccctg ctcgaatcat
      841 aagtcgatga ctggaaacct gcgcgtcaga cggatcctct ttttgtgtag gtaatatata
      901 ctgtgataaa cgcatttcta aattccttat ttataattat aatatctcta aaagccaacg
      961 atgtttatcc tcgatttcac ctgtctggat ctttgtcaaa gtttcgtaaa gttttgtcaa
     1021 aacaggtccg gcactatctt tttcaccaaa agttaaaatc ttatccccat actttatact
     1081 atgtacagga gttataaccg ctgcggtccc aactgcacct acttcatcga aaccttcgac
     1141 ttcactgatc tcgacctgtc ggctctcgac gagcattccc atatcttgcg caattgttgc
     1201 aagactcttg tttgtaatgc tgggtaaaat acttggtgac tttggagtaa tatacgaaga
     1261 gcctttgata cctataaaat tagatgtgct gaactcgtcg atatatctgt tatgttgtgg
     1321 atcaagataa agccctactg cacatccctg ctccttcaca aatttgctac cgggaagtcc
     1381 agccgcatag ttaccgccaa ctttacaatc cccaacacca tgaggagcag ctctgtcaaa
     1441 accttctaca ataatggcat ctacaggttt aaagcctcct ttataatatg gccctaccgg
     1501 tgttgcaaaa accagaaata tatattcatc tgctgggcca aggcctactt ttggtccgac
     1561 gccaatcata agcgggcgta aatagaggct ggctcctgtt ccatagggtg gaacaaatcg
     1621 tatattttct cgtattacag tctctacagc tgaaataaat atctcttccg gaatctccgg
     1681 catatttata cgactgtttg tccggttcat tcgctttgcg ttttcatcag ggcgaaaaca
     1741 gacaactctg ccgtctctgg tttcaaaagc cttaagtccc tcaaaccctt gctgtccata
     1801 atgaagcgcc ggcgcagcaa tattaataga caacatgtct tcttcacata tttctggagc
     1861 tccccaacta ccatttttat agtgatatct cacattttta tcggttttta catacataaa
     1921 aggtaaattt gaccagtcaa ggtcggcttt aacaacactc attgttttct cctgggttca
     1981 tgataaacat aaataatatt acaacctgcg cacctgcatt gatttttaat aatcattaac
     2041 aaaagcaaga taagctaata acgcctgatt tagagcaata ctaaaggtca gacaggacga
     2101 ttattaatca gataactaat attgtcaaac gcttcaggtg tttcaagctc aaacaaaagt
     2161 ccatataaat catttttttt ccatactaca ctgcttttta actcaatatc cttaccgagg
     2221 caaacatgta gagttatctg atcatcattt ttcaacggaa attcagttaa aatgcaggcg
     2281 cctgtaactg aaacattctt caataagcaa ctatattttt gattgcggct atctggcgag
     2341 aaaaaatagt cgccgttaca atcatgacga tcacccgctc ttcgttcttt gtttaacaat
     2401 taactctcca ttaatgcaaa tactatcaag aagaaatatt cgcaagactc tttttaaaaa
     2461 agatcaatcg gaccaacatc cgaaccttca ataaactgtt tagcgtaggg atcatttttt
     2521 gaaaaaaaat cttctacttc cagcatacat gctatcttgc ctccataaag caatatcacg
     2581 gtatctgcga tttttctagc aaatttcaga tcatgagtta ctgttacaga cgtaatagcc
     2641 gcgctctccc gtattcccaa gataaggtca gcaactgaat ctgtcaacac gggatcaaga
     2701 cccgcagccg gatcatcata aagcataatt tttggttcaa gacataaaca tcgtgccaaa
     2761 gaaaccctct tttgcattcc acctgataac tcagaaggat atttctcttc ggcaccggat
     2821 agtccgacac tttccaatac tatttttatc ttattatcta taacactttt gtcatatttc
     2881 tttcgtaaca ttccgaaagc gatattttcg gcaacagtca ttgaatcgaa aagaccaccc
     2941 ttttgaaaaa cataaccaac tatggatcgt tcgttcgagc cattactata aagagctggt
     3001 ccgactttaa cattatcaat ttgtatatcc ccttcattaa tactaattaa tccagcgata
     3061 gacttaagta atacactttt gcctgatcca tttttaccaa gaactaccag attacccttt
     3121 tcgggcacgt caaatgatat attatccaaa acttttattt cgtcaaaaga gaccgcgaca
     3181 tttttaacat gtatcatcgg tacttctgct cccgatactc taagagtgat tccagcatat
     3241 tgagataaga gacataacgt tctctgcata caatacccga ctcaacccga tctttaacac
     3301 cgcatccggg ttcatgctcg tgtgtgcaat ttgaaaaggt gcaatcagat cggtattgag
     3361 taaactcata aaaaaaattt cctagctctt ccggctctat gtctaccaat ccaaactctc
     3421 gcacacccgg cgaatcaata agctcggtac catctttctg cttatacatt ataacattcg
     3481 ttgtagtatg gcgccctttg ccagtcttat ctgaaatatc tgaaacagca agattaagat
     3541 cctgatatat cgaatttatt agcgagcttt taccaactcc cgaataaccg gccaataaag
     3601 tccttttacc ggttaacatt tttttaaggc tatcgattcc ctgcatattc aaagcacttg
     3661 tgattataat cttaagacca gaattcttgt aatattctat tactgtattt gtcatctctt
     3721 cggtagcaag atctgccttg ttcatgcaca aaaccggttc aatattttct ttgattgccc
     3781 ttacggcgag tctgtcaaca aatcgcggat taacaggtgg atccagaaaa gattgaataa
     3841 caagaacgac atcaagattg gccgctatta cgtcttcacg ggcatttcgt cccttgtcac
     3901 gtcttgaaaa cagattacta cgcggcaaca ggtcaatgat cgaaccgctt ccatcatcat
     3961 tatactgaaa tttcacatta tcaccgaccg caaccggatt tgtgaatttt ttgaactcct
     4021 tatttatacg aattttccct tttaaaaagc aggcaatatc tttttgtccg tcattgactg
     4081 tatagtatag cccaaacact ttgtttatta tgccggttcc aagattttcc tgacttgaat
     4141 tactcatatt atttccttaa taaaagagaa aagcctctaa ttatccataa ttaaaacttt
     4201 ttccacgata atcccctggt tcatcacttc aataagtttt tatgctattt ctcttctctt
     4261 cgggttttat tccgatagtt tactgaagat tattgataag gatctaataa tcacagaatc
     4321 taacgagcaa aatattttta aattttaaaa aaaaattaaa aaaagctaaa gtattgccta
     4381 tctataccga acataattga agggaacagt ctgtaatatc cctcccgata gacattactg
     4441 ttccctgcac tgcgcgtcca aacatgcaga gaaggcagaa tagccgccca gctattctgc
     4501 cctctctctt gttacccatt atttgaaaag tcgtgtagat aataataaca gtccggcaaa
     4561 accggactgc ttttttatta gaggaacttc ttaaactgca gtaattatta aatgtaacag
     4621 caatacagac ctgcaataca aatataatca catactcaat tttatatcta gtgaagcata
     4681 aataaacaac tttttttatt cgcaacaata actagtggtc acatccgtta gcacctgtta
     4741 caagtgttcc gtcaagatat gactgtacaa tctgtttcgg tgtttcggat ggagcaccaa
     4801 ccataacttt gattccctgt gaaataaaca actgctgtgc cctctgtccc atccctccgc
     4861 aaattaccat atcggcttca cgctctttta cccacttcgg caaaagaccc ggctcatgag
     4921 gcggagcgtc ttctcttttt tcgtctgtta tctcctttgt cgtatcatca aaagtaacaa
     4981 atgagaaata ggcgcagtgc ccaaaatgca gtgacagttt atcttctaca caaggtattg
     5041 caattttcat aaatatctcc ttaaatataa ttattctcga aaatcaacca aataagttaa
     5101 ccttacaatt aagcctttta attaacagcg ttaaatctta tatcagcaaa atgtgttgtc
     5161 attaatttat caacagcatt tttaaacccc accgctgctt cgtcagacga atctccagaa
     5221 attgaaaatg gttttccact atccccgcca tcagatacaa ccagagatat cgggatttct
     5281 ccaagaaaag gtatagagct tatctcagat atttttttac cgccacctct cttaaaaaga
     5341 tcagaaactt ttccacaatg agggcaccag aaaccactca tattttcaat tacgcccaat
     5401 cgaggaatat tgagtttttc acaaaaagtt accgattttg caacatctac cagcgccaaa
     5461 tcctgaggtg tggttacgat taccgctcca tcaggatttt caataagctg acaaaccgcc
     5521 aatacctcat cacctgttcc gggaggcgaa tcaataataa gaaaatccag atccccccaa
     5581 cttacatctg tcaaaaactg tttgattacg ttgtgcttca ttggccctcg ccaaatcatg
     5641 gcatcagacc ggctatcgat cagaaaacca atagacacag ctttgaggtt ttcattgtaa
     5701 tctatgggtc taatcttgcc cgattcatcc tgcgtatgat gttcaccttc catacccagc
     5761 aaggttggta ccgaggggcc atgaagatcg acatccaaaa gccccgtttt aaaacctttc
     5821 tgggcaagag acaatgccag attcacagcc acagtacttt tgccaacacc gcctttgccc
     5881 gacataacaa taatcttatg atcaacaccg ctcatgtttt cttttagttt ttcatcaatc
     5941 accttaactc tcctgtttta acaaatcctt tcgaattttt tcccatactt ttttcacctc
     6001 atctgcagcg ctactccctt cataactcac aactgagcgc ctgtcacgct gtgattcatt
     6061 gaatatcgtt gaatagttta tacggccagc aaatgaaaga tcattatccg cggcaaattt
     6121 ctcaatttgc tcacatattt caatattaat atctgcccgg tttattacaa ttgtaacggg
     6181 gatattcata cctgttacaa ggccggccgc ccgtttaaga tcactcaaac cagagagtgt
     6241 cggttcggtt actataacaa ctcccgttgt agctgtaacc gaagcaatta ccgggcaacc
     6301 tactcctgga ggtccatcaa taatcagcat tgaagattca atctcttcag catgtttttt
     6361 agcaatttcc ctgactcggc taaccatctt gccagaattt tcttcaccag ctattagcct
     6421 tccataaaca aagaaaccgg cattgctttt tgcacgatgg atcatgccac aattttttgg
     6481 gctttttctt acagcttcac ttccacaaac atagtaacat acgttacaac cttcacacaa
     6541 aaatgagtca attttaaaat cttttattgc accaaaccgg caaagttttt cgcatttcga
     6601 gcaatgcacg cacttttccg catcaatttc ataatcaaag ccagcaataa agtcttcgct
     6661 ctctactata tcgtgatcta gcgaaacaaa caggttggct ccgtcaacat cacagtcagc
     6721 cgcataggca tttttttcaa gttcaaaaaa agcacgggtt aaagaagatt ttccggtacc
     6781 tcctttcccg cttataacag caattgtatc aaccattttc cttctccgac attatcagac
     6841 ttttcaaacc atctacagca cccttgtaat gagaaatatc ttcatttttc agatatcctt
     6901 tcaaatcacc tatcgaatag tatcgcgaca gtttttcgtc aaaaggtact gtggccaaaa
     6961 caggcacatc aaggctctta cataatttat aaaaattttc tgaatacccc tcgtccttgt
     7021 taacaacaac agcactatca atattcatta ccttggtaag ttcgtgaatc aatttgaaat
     7081 cacttgttcc aaaaggtgtt ggttcagcaa ctattagcgc aaaatctgcc ccgtaaagtg
     7141 cctgtactgt attacaagct gttccgggag gcgcatctac aatctgaacc ctgtcactac
     7201 tcttatcagc caaaagttct tttatcagca ttggggactg aatttttccg acatctattc
     7261 ttccctcttt aaattcaata ttttcagagt taccgctacc aatttcacca tatttatcat
     7321 caacttcggt aagtgcatca acagggcaca cctcaacaca accgccacat gagtggcaaa
     7381 gatcagtaaa aatgagcggt ttctgtttca ccataaccac tgcattatac gcacaaatat
     7441 ctgcacattt gccacatcct gtgcatattt cactatttag acggggtatc tttcgataaa
     7501 cactttttga ttccagatta actccaagaa aaaggttcga atttggctct tcgacatcag
     7561 aatcaattag ttcgatatca acctcgttgc ttagcagaag ggacagtatt accgaaaatg
     7621 tggttttacc cgcccctcct tttccgctta ctacaacaat tctcatattt tattttctcc
     7681 ctttatagct tttcagatac tcatgccttt caaccgaaac aattaaatgt ttcggtttgc
     7741 tttaacagag ctaataaagc atcgatttag ttacaaattt tgtaattaca aagacttctt
     7801 ataaaacgaa actgtctccg gcagctccat atatacaatc cgctgtaacc ctgctcttta
     7861 aatcatggta cccgtcaaca ccggtacagt gaccacagat cagtcttgat acaccaaaat
     7921 gattcacagc tgccgcacaa gactcaattt gctcaatgga tgcccctttt aaatggaacc
     7981 ctcccattac tgtatggaaa cgtcttattg agaattgttc tgatacatag gttaatattg
     8041 atccgagacc tttgtgagca caagaggtaa caacagagta tccaagatca ccgtttacaa
     8101 gtagcaccga ttcatcttca aaataatcag gcttatattc ttcaccatct tttacccaaa
     8161 gcttgtgatt attcaatgct cccggaacgc ttttaccaag cggcaacaca gtgcattcac
     8221 ccacaactaa tggttcctct attaagttaa aattgattcc gtttttttca tactccattt
     8281 tactatgttt cataccaata gaacggtatt ctcctgtcga agaagagaac gcctccctga
     8341 ataaccggtt tgaaccaaca acagtaggat tactttgtga ttcacctata gcattgatca
     8401 aaggaattaa tccgccagta tgatcataat gacaatgcga caataccaga gcattaatag
     8461 ataccccggc atttttcatt tttttataat ttaaactagc agcgttagtg gcgccggcat
     8521 caagaatcag acttttttgc ccctctaaca aatacgagag accatgctcg gccaaaagtc
     8581 cgttccgata cgtacagtta tcgagtataa ccgatatttt cactaccagt ggccttccac
     8641 gtccgcttct gaagccaata caagtttacc atcacggtac atctgaagag cattttcaac
     8701 cgtaccttcg gcacctgtat acatggtaac atcggcagcc tttaataccg aaaaggcttt
     8761 tgggcctaca tgcccgctta tcaaaaccga aacaccagca tcaagaacag ttccggccgc
     8821 ttgaataccg gctccttgtg gagcattata gttttgacta ttacctaaat aactccaatt
     8881 tccactatcc tcatcaaaaa tcataaaacc ttta
//
LOCUS       DAOTGZ010000107         8896 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_3064
            NODE_9395_length_8896_cov_3.987219, whole genome shotgun sequence.
ACCESSION   DAOTGZ010000107 DAOTGZ010000000
VERSION     DAOTGZ010000107.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868088
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 8896)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8896)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:22:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,785
            CDSs (total)                      :: 2,751
            Genes (coding)                    :: 2,732
            CDSs (with protein)               :: 2,732
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 12 of 19
            Pseudo Genes (internal stop)      :: 5 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8896
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9395_length_8896_cov_3.987219"
                     /isolate="AMR_MDS_3064"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(422..1126)
                     /locus_tag="P5123_08105"
     CDS             complement(422..1126)
                     /locus_tag="P5123_08105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016400441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HRX16261.1"
                     /translation="MIDIRDKWALVTGASRGVGFRVARALSKKGANVILHSRKLEGTA
                     VLYDELKSEGSVVKQVEGELGDPFALQNIITEVSKLASAGLDILYNNAAIMTTYRNIY
                     EVTVDEYMQSFMVNCVAPAKLCDAFLPGMLKKNFGRIVNVTSGIADQPQLMAYSCSKA
                     ALDRYVRDMLPTLDKTNVLMNLMDPGWLSTDLGGDKAPNHPDTVLPGALVPVLLEQSE
                     GSGKLYCAQDYREDIK"
     gene            complement(1149..2318)
                     /locus_tag="P5123_08110"
     CDS             complement(1149..2318)
                     /locus_tag="P5123_08110"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003512097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0018455 - alcohol dehydrogenase [NAD(P)+]
                     activity [Evidence IEA];
                     GO_function: GO:0030554 - adenyl nucleotide binding
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron-containing alcohol dehydrogenase"
                     /protein_id="HRX16262.1"
                     /translation="MENFDFYNKTKIVFGKDVENKAGEECKAIGSKVLLHYGGGSIKK
                     SGLYDRVVKSLKDAGVDYVELAGVKPNPRLSLVKEGIQICRKENIDLILAVGGGSVID
                     SAKAIGVGVHYEGDVWDFYMGKASPEKMLPLGVVLTIPAAGSEASPSSVISNEEVQLK
                     KGLTNICLRPEFSLLNPELTYTLPDYQTACGITDMFAHVMERYFTTTKGVELSDRLCE
                     SVMKTVVHQAYRVLKDPKDYTARSEIMWAGTIAHNDMIGCGRSQDWSSHGIEHEISAI
                     YDVAHGAGLAVVFPAWMKHVYTNNIGRFARFAHEVFNVDYDFEKPERTALEGIAALER
                     FFKDIGMPVRLSEMDIDDKNIETMASKVTGNGAHSIGGFKELSKDDVKAILKLAL"
     gene            complement(2445..3599)
                     /locus_tag="P5123_08115"
     CDS             complement(2445..3599)
                     /locus_tag="P5123_08115"
                     /EC_number="3.1.3.16"
                     /inference="COORDINATES: protein motif:HMM:NF018884.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004722 - protein serine/threonine
                     phosphatase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PP2C family protein-serine/threonine
                     phosphatase"
                     /protein_id="HRX16263.1"
                     /translation="MKFNKIFSQNQVGFIAEIVEPVHHSFNIDYVKNEFLKDPELSAL
                     PVETESGISGFIERDAVLKDQKKLDSILHKSIKNFVVSNTLFLKARENIEKALKDIFL
                     SSKTQINNCMVYHNGDFCGIVNTRKLVAHILKLRNNELKRAREVQKKILGHNKMDNSL
                     FKSEVVIEMAHEVGGDFFLMKNLGDHKYIISCMDVSGKDVSASLITGVVGGYFASLEY
                     FTNLSSLSGTEIITALHNIIRARTPEGYFLAGVVVVIDSEKKEIEIFNMGYSPLCMVY
                     KNTGENRIVIKNPTLPPIGLPNFKISDDLVIRENIKKGFSLFGFSDGLTDAQNSHGIA
                     LGDKAVYELLKQSLKKNDQDALNSVVKEVKNYIGTAPFADDITAFMVNFK"
     gene            3852..4907
                     /locus_tag="P5123_08120"
     CDS             3852..4907
                     /locus_tag="P5123_08120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013451571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="agmatine deiminase family protein"
                     /protein_id="HRX16264.1"
                     /translation="MEIRLPAEWEQQSAVMLAWPHSDSDWAQYLDEAKKCFSQIINTI
                     AKYQDVIVVQNTTSIDDNISVIANTPRVYTIKARSNDTWCRDYGFITVLDKGKPCLYD
                     FKFNGWGLKFASNYDNMINNHLYAQGLFINAKYVNMLGFVLEGGSIESDGIGTIMTTS
                     DCLLSPNRNGGFSCQEIEDALRKFFGAKRVLMLNSGYLSGDDTDSHIDTLARFCSTDT
                     IAYVKCDDKSDPHYHSLKEMEKELQEFTTPKGIPYNLVALPMANAEYYNDERLPATYA
                     NFLIINNAILMPGYGTSLDIKAQAILRKLFPGREVYIIDCSVLIRQHGSLHCVTMQFP
                     ENIKLNRRFCNDRKEIQ"
     gene            4885..5790
                     /locus_tag="P5123_08125"
     CDS             4885..5790
                     /locus_tag="P5123_08125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008140297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbon-nitrogen hydrolase"
                     /protein_id="HRX16265.1"
                     /translation="MIEKKSNKNILMAGLIQQKCTSNIDSNKKLLRTNAEQLAQMGAN
                     IIIFQELHNSLYFCQAEDIAMFELAEPIPGPSTEYYGTLAKELNVVLVTSLFEKRAPG
                     LYHNTAVVFDKDGSIAGTYRKMHIPDDPGFYEKFYFAPGDKGFVPIETSIGKIGVLVC
                     WDQWYPEAARLMALAGAELLIYPTAIGWYPPDSADEKKRQQSAWITIQRSHAIANGVP
                     VIAVNRVGNESLEGEAGIDFWGSSFVAGPQGEILAQASSSNEENMIVPINLANSEIVR
                     QTWPFFRDRRIDDYTELTKRYIDKI"
     gene            5832..6581
                     /locus_tag="P5123_08130"
     CDS             5832..6581
                     /locus_tag="P5123_08130"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRX16266.1"
                     /translation="MSKANIAFFILYIVLILTGIVFSATQLNDIYDYEFPDKIESGKL
                     MRMKHGCFYLPKGYNSKKQYPLVITLHPMSTGEETFIYSNNLTDYADKRGYILLAVRA
                     TKLVWEEEPDSGTILKMASVIKTKLPINKKRILLTGFSSGGHFTLTMLLYNRRFFTSE
                     TLFTAFAAGGGGAGYELGIMMGKNRTPNPKTPGYIYWGIKEPGVPGKEVSEYLLNHGW
                     NIVANEHPGHHFVPDGEFERIFDWFDNLELN"
     gene            complement(6585..7775)
                     /gene="cls"
                     /locus_tag="P5123_08135"
     CDS             complement(6585..7775)
                     /gene="cls"
                     /locus_tag="P5123_08135"
                     /inference="COORDINATES: protein motif:HMM:TIGR04265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008808 - cardiolipin synthase activity
                     [Evidence IEA];
                     GO_process: GO:0032049 - cardiolipin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cardiolipin synthase"
                     /protein_id="HRX16267.1"
                     /translation="MKKKSATSQYWMRNALPLYLKQMKKLNIGGFSSGNQVELIVDGA
                     ECYKKFYDAIDNAVSSINLETYIFRSDEAGWEIANRLAAAADRGVEVNLIYDAIGSIK
                     TSRSIFNFMRSMGVEVIEYHPIFPWRKFWNITFRDHRKIMVIDGKCAFIGGLNIGNEY
                     AGNLYTDEYQRDTHARITGPAVREIQFFFMENWFRNGGSVVDYSRHFPDVKKSGKKMV
                     MILCTKSRRNVRPIHFSYLSAIDSARESISIANAYFIPDQKIYRSLCAAAKRGVSVKI
                     ILPSRYAMPVVKNAVRYLYRRYLRHGIEIYEYQNNVLHVKSAVIDSVWSMVGSSNLDR
                     LSYRINLELNAVVLDEDFGEKMEKVFHDDLKYCQKIKIENIEKRSLWRLFVEWLCYRF
                     RNIL"
     gene            complement(7816..>8896)
                     /locus_tag="P5123_08140"
     CDS             complement(7816..>8896)
                     /locus_tag="P5123_08140"
                     /inference="COORDINATES: protein motif:HMM:NF019344.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit"
                     /protein_id="HRX16268.1"
                     /translation="EDVIDVISAWSGVNIDKLSMSESERLLNLEDEIHKKIVGQDEAV
                     RALCGAIRRSRTGFRRSNRPIGAFVLAGPTGVGKTELAKVLAEYLFGNKNSLIRLDMS
                     EFMEKHSVSRLVGAPPGYVGYEEGGQLTEKVKRRPYCVILLDEIEKAHPDFMNIMLQV
                     LDEGELTDNMSGTVSFRDTIIIMTSNLGNSRFDKTARTGFLDSTETEGTSQEKVREAV
                     KGAFNPEFINRIDEIVCFHHLEDSHILKILDLMLSEISDLVKDKDVELVFTAALKKYL
                     AEKGMSHQYGARYLRKVIQDEIEDALALEILRGGIPEKSKVTVSTRAGKVTIKTKKNS
                     FSEQNNSPLINNEKVNGELSELEKI"
BASE COUNT         2772 a         1929 c         1517 g         2678 t
ORIGIN      
        1 gattatattt tttttcatca aataaccatc cataaaataa gaaataacca aaaaatttac
       61 atcgcctaca aaaagccttt gtgcttaaga taaaaatttg aaactaaaac ctttataaaa
      121 ttactgtttg caaatcacat gtgaattccg aacgagcaac aatatgagaa tatactatta
      181 tagcaacact atttcgatct ttaaataaaa ctttgcctgc caacgacact tgctattcgt
      241 aatttcaata aaaaaaggga taagcacaat atgctccacc cctttaataa tttcaaaaga
      301 ttatagtaac ctctgataat atcaccgaaa caatgtagat ctctagaaca ttgtaatcat
      361 tgaagttact atagtcaaat attctcccag acaagcgagg actgctcgat tacctgacca
      421 atcactttat atcctctctg taatcctgag cacaatatag tttaccacta ccttcgcttt
      481 gttccagcag aactggcacc aaagcacccg gaagcaccgt atcagggtga ttaggagcct
      541 tgtctccacc aagatcggta cttagccaac ctggatccat cagattcatc agcacatttg
      601 ttttatcaag cgttggcaac atatcacgta catatcgatc cagtgcagcc tttgaacagc
      661 tgtacgccat aagctgaggc tgatccgcaa taccacttgt tacattaaca attctaccaa
      721 agtttttctt aagcataccc ggtaaaaaag catcacataa tttagcagga gcgacgcagt
      781 ttaccataaa actctgcata tactcatcga ctgtaacttc atatatgtta cgataagtag
      841 tcattatagc agcattgtta taaagaatat caagaccagc agacgctagc ttactaactt
      901 ctgtaattat gttttgaaga gcaaacggat caccgagttc accttctacc tgtttaacga
      961 cactaccttc tgattttagc tcatcataaa gaactgctgt tccttccagt ttacgtgagt
     1021 gcagtattac atttgcgcct ttttttgaaa gagcccttgc gactctaaac cctactccgc
     1081 ggcttgctcc tgtaacaaga gcccacttat ctcttatatc aatcatttaa taactgctcc
     1141 tttaaaaatc atagtgcaag tttcaatatc gccttcacat catctttgct tagctcttta
     1201 aaaccaccaa tactatgagc tccgtttccg gtaacttttg aagccatcgt ttcaatattc
     1261 ttgtcatcaa tatccatttc tgataatctg acgggcatgc ctatgtcttt aaaaaatctt
     1321 tcaagagccg caatgccctc aagagctgtt ctttctggtt tttcaaaatc ataatcgaca
     1381 ttaaacactt cgtgagcaaa tcgagcaaat cgcccaatat tatttgtgta aacatgtttc
     1441 atccatgcgg ggaaaaccac agcaagtccg gcgccatgag ccacatcata aatggcgctt
     1501 atttcgtgtt caataccatg cgaactccag tcttgcgaac ggccacaacc gatcatgtcg
     1561 ttgtgtgcta ttgttccggc ccacataatc tctgatcttg ctgtataatc cttgggatct
     1621 tttaaaacac gataagcctg gtgaactacg gttttcatta cagattcaca aagtctgtcc
     1681 gaaagttcca ctcctttggt tgttgtaaaa tatcgttcca ttacatgcgc aaacatatcg
     1741 gtaattccac aggcagtctg ataatcagga agggtataag tcagctcagg gttcaataga
     1801 gaaaattctg gtcttaagca aatatttgta agtccctttt taagctgaac ctcttcattt
     1861 gagataacag aacttgggct tgcctcactt ccagccgcag gaatggtaag aacaactcct
     1921 aacggtaaca ttttttccgg tgaggccttc cccatataaa aatcccagac atcaccctca
     1981 taatgcacac cgacaccaat tgcctttgcg gaatcgatta cacttcctcc gcctacagcc
     2041 aaaataaggt cgatattctc tttccggcat atctgtatac cctctttaac aagagacaat
     2101 cgtggattag gcttaacacc tgcaagctcg acataatcga caccggcatc tttaagtgac
     2161 ttaacgaccc tgtcgtaaag cccgctcttt tttatgctac caccgccata atgcagaagc
     2221 actttagaac cgatagcctt acactcttct ccggctttgt tttcaacatc tttaccgaaa
     2281 actattttgg tcttgttata aaaatcaaaa ttttccataa tatcctctta tataagttat
     2341 tttttggcgc atcactctaa cacgtgttat attgtgacaa tataataaac acccctaatt
     2401 attcaagaac atttattaca atgtatcgat catctgaaac ataactattt gaaattgacc
     2461 ataaatgcgg ttatatcatc ggcaaaagga gcagtaccta tgtaattttt aacctcttta
     2521 acaacgctgt taagcgcatc ctgatcgttt ttcttcaggc tctgtttcag caattcgtaa
     2581 acagctttat cgccaagcgc tatgccatga gagttttgcg cgtcggttaa accgtctgaa
     2641 aagccaaaaa gtgaaaaccc ttttttaata ttttcacgaa taacaagatc atcacttatt
     2701 ttgaaattgg gcaaccctat ggggggaagt gtcgggtttt taataactat ccggttttca
     2761 ccggtatttt tataaaccat acagagtgga gaatatccca tattaaaaat ttcaatctcc
     2821 tttttttcac tatcaattac aaccacaact cctgccaaaa aatatccctc cggagtacgt
     2881 gcccgtataa tattatgaag agccgttatt atttcggtac cacttagcga tgataggttc
     2941 gtgaaatatt caagggaagc gaaatagccc cctacaaccc cagtaataag cgaagccgag
     3001 acatccttgc cactaacatc catgcatgaa attatgtatt tatgatcccc aagatttttc
     3061 ataagaaaaa aatcaccacc aacctcgtga gccatttcta ttaccacttc tgatttgaat
     3121 agcgagttat ccattttgtt atgaccaagt atctttttct gtacttcacg agctcttttt
     3181 aactcgttgt tacgaagttt aagtatatgt gccacaagtt tacgtgtatt aacaataccg
     3241 caaaaatctc cattatgata aaccatgcag ttatttatct gtgtttttga tgacaaaaat
     3301 atatctttaa gcgccttctc gatattttcg cgagctttaa gaaatagtgt gttgcttact
     3361 acaaaatttt tgatactctt atgtaaaatg gaatcgagtt ttttttgatc ttttaaaaca
     3421 gcgtccctct cgatgaatcc gcttataccc gattcagttt caacaggaag agcactcaat
     3481 tcgggatctt ttaaaaattc atttttcaca taatcaatat tgaaagagtg atgaactggc
     3541 tcaacaatct ctgcaataaa acctacctga ttttgggaaa atattttgtt aaacttcatg
     3601 tataccccga ttgacaaagt agattggaac ctctattaat taacttttca acaagaaact
     3661 tgcgaaaaat tctaatagaa taggcactta aaagttgatg tttccgaaaa aggtaatttt
     3721 tcgaagctcc atggttgtca tttttattta aaaaagttca agaaaactgt ttgtaaatat
     3781 gcatgtgttt tattaaattg caaccaatat ttttaaccga ctatattata ccttgttatg
     3841 aggcttaccc tatggaaatc cgtcttcctg ccgaatggga acagcaaagc gctgtgatgc
     3901 tcgcatggcc acacagtgac agcgactggg ctcaatacct tgatgaagca aaaaaatgct
     3961 tttcccaaat tattaacaca atagcgaaat accaagatgt catagttgta caaaacacca
     4021 ccagtatcga cgacaacata tccgttattg caaacactcc tcgggtttat accatcaaag
     4081 ctaggtcaaa cgatacctgg tgccgtgatt atggatttat tactgtttta gacaaaggta
     4141 aaccatgcct ttatgatttc aagtttaacg gttgggggct taaatttgcc tctaattacg
     4201 acaatatgat aaacaaccac ctatatgcac aggggctttt tattaatgcc aaatatgtta
     4261 acatgcttgg ttttgtactc gaaggcggtt ccatcgaatc agatggtatt ggaacgatca
     4321 tgaccacgtc tgactgtttg ctttcgccga accgtaacgg tggtttttct tgtcaggaaa
     4381 tagaagatgc tctaagaaaa ttttttggcg cgaaacgtgt acttatgctc aattccggat
     4441 acttgtccgg cgatgatacg gatagccata ttgacacact tgccagattc tgttccacag
     4501 atacgatcgc atatgtaaaa tgtgatgaca aaagtgatcc tcactatcat tcgctaaaag
     4561 agatggagaa agaactacaa gaatttacca caccaaaggg cataccatac aatcttgttg
     4621 ctcttccgat ggcaaatgcg gaatactaca acgatgaaag acttcccgcg acctatgcca
     4681 attttcttat tatcaacaac gcaatactta tgccgggata cggaacatct cttgatatta
     4741 aagcacaagc tatacttcga aaactttttc caggtcgtga agtttacatc attgattgct
     4801 cggtattaat aagacaacat ggatcactgc actgtgttac aatgcagttt ccggagaata
     4861 ttaagcttaa caggagattt tgtaatgata gaaaagaaat ccaataaaaa tatactcatg
     4921 gcaggactga tacaacaaaa atgcacctct aatatagata gtaacaaaaa attactaaga
     4981 acaaacgccg agcaacttgc acagatggga gcaaatataa ttatctttca ggagcttcac
     5041 aattcattat acttctgtca ggcagaagat atagcgatgt ttgagcttgc ggagcctatt
     5101 ccagggccat caaccgagta ctatggcacg ctcgctaaag agctaaatgt tgttttggta
     5161 acatcacttt ttgaaaaaag agctcccggt ctctatcaca atacggctgt tgtttttgac
     5221 aaagatggct ccattgctgg aacatatcgc aagatgcata ttcctgatga tcccggattt
     5281 tatgagaaat tctattttgc accaggcgac aaaggttttg taccaataga aacttcaatt
     5341 ggcaaaatcg gcgtacttgt ttgctgggat caatggtatc ctgaagccgc ccgtcttatg
     5401 gcgctggcag gtgcagaatt gttgatttac cccaccgcta taggatggta tccccccgac
     5461 agtgctgatg agaaaaaacg tcagcagagc gcctggataa caatccagcg gtcacatgca
     5521 atcgcaaacg gagttcccgt tattgctgtt aatcgtgttg gtaacgagtc tctcgaaggc
     5581 gaagctggta ttgatttctg gggaagcagt tttgtagcag gcccgcaagg tgaaattctg
     5641 gctcaggcat caagcagcaa tgaggagaat atgatagtac ccataaatct tgcgaattcc
     5701 gaaattgtac gacaaacatg gccatttttc agggatagac gcattgatga ctacacagaa
     5761 ctaaccaaac gctatatcga caagatttaa aaatcacgaa gaaaacttta tcacccaaag
     5821 aggtcataac cgtgagcaaa gcaaatatag cgttttttat tctatatatt gtattgattc
     5881 ttacaggtat tgttttttcg gctacgcaac taaacgatat ttatgattac gagtttcctg
     5941 ataaaattga atccggaaaa ctaatgcgaa tgaaacatgg ctgcttttat ttgccgaaag
     6001 gttataatag taagaaacag tacccactgg taataacctt acatcccatg tcaacagggg
     6061 aagaaacttt tatatacagc aataacctta ctgattatgc cgataaacgc ggttatattc
     6121 tgcttgctgt cagggcaaca aaactcgtat gggaagagga acccgatagt ggcacaattt
     6181 taaaaatggc atctgtaatc aaaaccaaac tcccaattaa caagaaaaga atactgttaa
     6241 ccggtttttc ttcgggtggt cacttcacat taacaatgct gctttataac cgtagatttt
     6301 ttacctcaga aactcttttt accgcattcg cggctggagg cggtggtgca ggctacgagc
     6361 ttggcatcat gatgggaaaa aacagaacac caaatcccaa aactccagga tatatctatt
     6421 ggggcataaa ggaaccagga gttccaggca aagaggtgtc cgaatatctt cttaatcacg
     6481 gctggaatat tgttgcaaat gaacatcccg gtcatcactt tgttccagat ggcgaattcg
     6541 aacgtatttt cgactggttt gacaatctgg aattaaactg attcttataa aatatttctg
     6601 aatctgtaac agagccattc tacaaacaga cgccataaag aacgtttttc aatattttct
     6661 attttaattt tctgacaata ttttaaatca tcgtgaaaca ctttctccat tttttcaccg
     6721 aaatcttcat caagaactac agcgtttaac tcaagattga ttcggtagct aagacgatcc
     6781 agattagatg atccaaccat ggaccagaca ctgtcaataa cagcactttt gacatgaaga
     6841 acattattct ggtattcgta aatttctatg ccgtgtctca aatacctgcg ataaagataa
     6901 cgtacagcgt tctttacaac tggcattgcg taacgtgatg gtaatatgat ttttacagaa
     6961 accccccgtt tagctgctgc acacaacgat ctgtaaatct tctggtctgg aataaaatag
     7021 gcgtttgcaa ttgaaataga ttctcttgcg ctatcaatag cggaaagata agaaaaatga
     7081 ataggacgca catttcgtct tgattttgta cacaaaatca tcaccatttt ttttccgctt
     7141 tttttaacat caggaaaatg acgtgagtaa tctacaacag atccaccgtt tcggaaccaa
     7201 ttttccataa aaaagaattg aatttccctt acagcaggcc cggttattcg agcatgagta
     7261 tcacgttgat actcatcagt ataaagattt cccgcgtatt cgttaccgat attcagtccg
     7321 cctataaacg cgcactttcc gtcaataacc ataattttac gatgatcacg aaatgtaata
     7381 ttccagaact ttctccatgg aaaaatagga tggtattcaa taacctctac acccatcgaa
     7441 cgcataaagt taaatattga acgagatgtt tttattgagc caatagcgtc atatataaga
     7501 tttacttcaa caccacgatc ggcagctgcc gcaagccggt tcgctatttc ccagcccgcc
     7561 tcgtcacttc taaaaatata tgtttcaaga tttatacttg atacagcatt atcaatggca
     7621 tcataaaatt ttttataaca ctctgccccg tcaacaatca actcgacctg attaccgctg
     7681 gaaaacccgc caatattcag ttttttcatc tgttttagat ataaaggaag ggcgttccgc
     7741 atccaatatt gcgatgttgc agatttcttt ttcataaaat tagttaacct ctaataatta
     7801 acttttttac aaacatcaaa ttttttccaa ttcggaaagt tctccgttta ccttttcgtt
     7861 atttataaga ggtgaattgt tttgttctga aaaactattt ttttttgttt ttatggtaac
     7921 tttgccagct ctagttgaga cagtaacctt agatttctca gggatccccc cacgaagaat
     7981 ctcaagggct agagcatctt ctatctcgtc ttgaataacc ttgcgaagat aacgcgctcc
     8041 atactggtgc gacatgcctt tttctgccaa atattttttt agcgcggccg taaagacgag
     8101 ctccacgtct ttgtctttta caaggtcgga tatttcagaa agcataagat caagtatttt
     8161 aagtatatgc gaatcttcca gatgatgaaa acacacaatc tcatctatac ggtttataaa
     8221 ttcaggatta aaagctcctt taacagcctc gcgaaccttt tcctgcgaag tgccctctgt
     8281 ttcggtacta tccagaaaac ctgtcctggc tgtcttgtca aagcgtgaat taccgagatt
     8341 cgatgtcata atgattattg tatcccggaa agatactgta ccgctcatat tatcggtaag
     8401 ctctccttca tctagaacct gaagcataat attcataaag tcgggatgag ctttttcaat
     8461 ctcatcaaga agtatcacac aataaggtcg cctctttacc ttttcagtaa gctgcccccc
     8521 ctcttcgtaa ccaacataac ctggaggcgc tccaacaagt cttgaaacag aatgtttttc
     8581 cataaattca gacatgtcca gacgaatcag tgaattttta tttccaaata aatattcagc
     8641 aaggactttg gccaactccg tcttgcctac tccggtagga ccagcaagta caaaagcgcc
     8701 aattggtctg ttagatcggc gaaaccctgt acgcgatctt ctaattgcac cacaaagtgc
     8761 tcgaactgct tcatcctgac caacaatttt tttatgaatc tcatcttcaa gattaagcaa
     8821 acgctcactc tcactcatac tcaatttgtc aatattaacc ccagaccaag ccgaaataac
     8881 atcgataaca tcttct
//
LOCUS       DAOTGZ010000108         8844 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_3064
            NODE_9462_length_8844_cov_5.032996, whole genome shotgun sequence.
ACCESSION   DAOTGZ010000108 DAOTGZ010000000
VERSION     DAOTGZ010000108.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868088
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 8844)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8844)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:22:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,785
            CDSs (total)                      :: 2,751
            Genes (coding)                    :: 2,732
            CDSs (with protein)               :: 2,732
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 12 of 19
            Pseudo Genes (internal stop)      :: 5 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8844
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9462_length_8844_cov_5.032996"
                     /isolate="AMR_MDS_3064"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..248
                     /locus_tag="P5123_08145"
     CDS             <1..248
                     /locus_tag="P5123_08145"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRX16269.1"
                     /translation="FYAQAAMLAANVFQAFARFNYSSALGADDGNDFTFTKLSLGGNY
                     FIDDHHAKATIQLDIPVGDSKDMDGEQKLSLQFQAYL"
     gene            complement(375..860)
                     /locus_tag="P5123_08150"
     CDS             complement(375..860)
                     /locus_tag="P5123_08150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015613523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HRX16270.1"
                     /translation="MVDVVIMKMINYFRGDIKRINHALKVYGFAGCIAGGENLSDKEI
                     KVINLAAVLHDIGIVNAQKKHNSTSGRYQEIEGPGVARQLMSDISIDVDILERVCFII
                     GHHHSYNKIDGDDFQIIVESDFLVNIDEENMSLKSIRAISDKYFKTATGTELIKNIYF
                     S"
     gene            1144..2979
                     /locus_tag="P5123_08155"
     CDS             1144..2979
                     /locus_tag="P5123_08155"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HRX16271.1"
                     /translation="MKRTSLRKIRIFSLGGSLFFILLTICSLWYADIMITYKKSISQL
                     SQGIRLRELDHIKISTTEQAAYLDNIANDMKEESLVFLASEVHKINDKIIQAERDYSS
                     PLTDGEIIKKISLSLNFASTRDPLISFITIHNNQFINKTETISYSAKTLYDYCKTNKE
                     GFFPYIKQKESQIILSDNVEYIYLKYYEPLDICFGAVLHTKAIDKKIKEAINKHLRIV
                     NTNKTDFFFTATIANNSLTEISSHWEIINSITPYNVHNNIFFQNLKSGYTGGFIKCDA
                     TSVLDGTTKLRLCYITPLVTRNQVLGFGKYKDDIDAIINADAQHLTKGRNRSLTVVAS
                     VLIVFIILSVIITVKLSGIIFKQTAIFKNNFLKATQDGTPLDLSSLYITELIEFADEI
                     NKLLATRKYSENMMKKSIAEKEILIKEIHHRVKNNLQIIISLIELQTLHTDNPSTITL
                     LKETSGRIRSMGIIHEKIYQTKDLTEVPAKDYFSTIVDELMFSYNIDHNKIKIVKNID
                     DITFNMDTAIPLGLIINELLTNTIKHAFKKDQEGRIHISAKKFANRTEIIVSDNGIGI
                     PIKEEMDKRSRLGMQLIRGLTMQLGAELSIIAKNGTKTKITLQNT"
     gene            3238..5277
                     /locus_tag="P5123_08160"
     CDS             3238..5277
                     /locus_tag="P5123_08160"
                     /inference="COORDINATES: protein motif:HMM:NF014763.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein"
                     /protein_id="HRX16272.1"
                     /translation="MRINVRGLKFQILKNYVIIAAIPVLFTFFITSGLMKNNTQTRFL
                     DNSAMSVNISDLAIQTMINRYKHEFEMLARSQNLLSGSIYTFNSDKKTPVKDSLSGNA
                     ASDLINDLKHLTDDTTEITRVYAVKTNSSLFQFPTQKMAAPFAVTDSPLFKQALSSNE
                     TQISELYFDSELNTTLLTFARKLDSETVFFADIDFQKMTSFISSLNYEKSGYLVICDE
                     DGKIINNPRTPELNQQPITAIGISGLETVAKKTHDQLAGLDSKKISHVVNFFTSKEYS
                     WKYVILVKYDEVVDGVAFVFNITLFFIFLMVMIFILYGAKSAKYIIEPITTLREYLWS
                     ASKGDFHNTMSTKITKRKDVIGELAQSYNDFITITRDVISDVNHSANQLAYSADEISS
                     AVKDFSANIQNQSANSEEISASVEEMEASMINVAGESETQNNSIKYLDKQIKDMSVML
                     REIQKMTQNTFTLTEKLQSRAATGEQTLRTMDHTMVKLNNSSQDMVNILAIIDEISEQ
                     INLLSLNAAIEAARAGDAGKGFAVVADEISKLADQTAKSLKDIDSLIRMNSSEISDGQ
                     KNINETLLLITDFLSGINSINDMNRKIGNFMTQYSQTNDEVSNRAENVQKISEGIKHA
                     ADENKRAISEIARSIFDINDLSQKNATHSQDVSDNTKKLAAMSESLKSKIKFFKI"
     gene            5680..6423
                     /locus_tag="P5123_08165"
     CDS             5680..6423
                     /locus_tag="P5123_08165"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRX16273.1"
                     /translation="MTMNRENCRDFIKLYFSKPYLHADFDIIHKGIDCFKHIPEDIEP
                     EQYMNLLQVLIQKNGIFSYLRYFQDNHFALLCSVLHSITLQCIKKNDEKQNDQTEKGS
                     DKFVVPDIVELSFLFDCDLRTNPDLILKYNQNRIVQFRKALDMLLTEKRYVSAKSFKA
                     ITLFKYTADKNILIRAKNITRSFINDFPLSSQKLMLFLIALIALDENLQETEHIEELM
                     KLCSDDDFKRSISSAIFRLKNQRFHYKPF"
     gene            complement(6389..7486)
                     /locus_tag="P5123_08170"
     CDS             complement(6389..7486)
                     /locus_tag="P5123_08170"
                     /inference="COORDINATES: protein motif:HMM:NF012551.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endo-1,4-beta-xylanase"
                     /protein_id="HRX16274.1"
                     /translation="MISIKQIILLSGLLFFIGCSSPNLSDKPKTFKAKKMISQVYKEH
                     FLIGAAVASSEYGNRDYFDIRYAELLLHYNSLTAENAMKPEVIHPLKDQFNFKAADRI
                     INYASSNNINVRGHALVWHSQTSPWITEGSKDVVRENMRNHIMTVVGRYKGKIYSWDV
                     VNEAVSDQGSYRADSPWYKAYGGPEYIVDAFKFAKAADPDCSLIYNDYAVVRPSKRHD
                     IITMIKKLELKKHGLDGIGIQAHWNLYWPEINEIQRTINMFSEMGLDIHITELDIDCF
                     DGIGNKEGRAFDKDLDDKLAARYREIFELFRINSDKIASVTFWGIADDHTWLDYHYNK
                     TWNAGKPLKNYPFVFDTDHKAKKAYNEIVDF"
     gene            7874..8482
                     /locus_tag="P5123_08175"
     CDS             7874..8482
                     /locus_tag="P5123_08175"
                     /inference="COORDINATES: protein motif:HMM:NF012257.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0030552 - cAMP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HRX16275.1"
                     /translation="MSKMFKYYGINVQKGDYLFKEGDAADYLYMIHKGEILINRIING
                     VEEKIKILEEGEFVGEMAIIDSKPRSANAIALNDCQLIRMTKEAFEINYQSNPQLGMS
                     FLKFLSSRIRDTNEIVSFMLSKNRDQFFMLELFKLVTVSGKKDSTGNWFLVDYSNFVE
                     HMINRSNYNETEVISIIQEICKKDNVKLVNSKNKNQWIATKV"
BASE COUNT         3070 a         1670 c         1410 g         2694 t
ORIGIN      
        1 ggttttatgc acaagccgct atgctggccg ccaatgtttt tcaggctttc gcacgtttta
       61 attattcaag tgccttgggt gccgatgacg gtaacgattt taccttcaca aaactgagcc
      121 taggcggcaa ctattttatc gatgatcatc atgcaaaagc cacaatacag cttgatatac
      181 cagttgggga cagcaaagac atggatggag agcaaaaact gtcgcttcaa tttcaggcgt
      241 atttgtaata aaccattgat gaaagccctc aaatcataga gggctttccc atatctaagc
      301 aatacaggca gctaaagagc tagcccggca atcaaagtaa ccaaaaactt ttctgtctaa
      361 aaaaaattaa aagtttaact aaaataaata ttctttatca gctcagtacc tgtagctgtt
      421 ttaaaatact tatcgctaat tgcccttatg gattttaaag acatattttc ttcatcaata
      481 ttaaccaaaa aatccgactc aacaataatt tgaaagtcat ctccatctat tttgttatac
      541 gaatgatgat gcccaattat aaagcagact ctttcaagaa tatccacatc tatactgata
      601 tctgacatta attgacgagc cacaccaggc ccttcgattt cttggtaacg accacttgtg
      661 gaattatgtt ttttctgtgc gtttactatt cctatatcat gtaacacagc tgcgagatta
      721 attactttta tctctttatc agataaattt tctccacctg cgatacaacc agcaaacccg
      781 taaaccttca aggcatggtt tatacgcttt atatcgcctc taaaatagtt aatcattttc
      841 attattacca catcaaccat cgtattctcc caactaaaat gctgctttat ttagctaagc
      901 ataagcaaaa atgtctaaca atatttatta cagattgtta atatcagttt aaataataaa
      961 atatactatt tctttctact tgatccaaat atcaaacatc agaattgctt gtttccctta
     1021 ttgtttgtca aatttctttt gattcctata gaaaaagact tatatttatc tagtcgctgc
     1081 gatcacaatg gtaaaattat atttaattag tagctattaa ggcaataagc aaaaaggaat
     1141 acaatgaaaa gaacaagcct tagaaaaatc cggattttct cattaggtgg atcgctattc
     1201 tttattctat tgacgatctg ttctttatgg tatgctgata taatgattac ttataaaaaa
     1261 agtatttctc agctttctca agggatacgt cttcgagagc ttgaccacat caaaatatca
     1321 accacagaac aagcagccta tctggataat attgctaatg acatgaagga agaatctctt
     1381 gtttttttag caagtgaagt ccataaaatt aacgacaaaa taattcaagc tgaaagagat
     1441 tactcctcac cacttaccga tggcgagata ataaaaaaaa tatctttatc attaaatttc
     1501 gcgtccacaa gagatccact catatctttt atcacaattc acaacaacca atttattaac
     1561 aaaacagaaa ccatctctta ttcggctaaa acattgtatg attattgcaa aacaaataaa
     1621 gagggatttt tcccgtatat taagcaaaaa gagtctcaaa taattttgtc agataatgtg
     1681 gaatatatat acctaaaata ctatgaaccg cttgatatat gctttggggc agtcttacat
     1741 acaaaagcta tagacaaaaa aatcaaagaa gcaataaata aacatctgcg aatagttaat
     1801 acaaacaaaa ccgatttctt ctttacagcg acaatagcta acaactcatt aacagagatc
     1861 tcatcacatt gggaaattat aaacagcata acaccatata acgttcataa caacattttt
     1921 tttcaaaacc ttaaatcagg ctatactggt ggatttatca agtgcgatgc aacttcggtt
     1981 ctagacggca ctactaagct ccggttatgt tatattactc cactagtaac tcgaaaccag
     2041 gttcttggat ttggcaaata caaagacgac atagatgcaa tcattaatgc tgacgcccaa
     2101 catttaacga aaggaagaaa tcgttcatta acggttgttg ctagcgtcct aattgtattt
     2161 attatattgt cagttattat taccgtaaaa ctttcaggca ttattttcaa acagaccgct
     2221 atcttcaaaa acaacttttt aaaagcaaca caagatggaa caccactaga tttatcttca
     2281 ctttatatta cagaactaat agaattcgca gacgaaataa acaaactgct tgctactcgg
     2341 aaatattcag aaaacatgat gaaaaaatca atagccgaaa aggaaattct aatcaaggag
     2401 atccatcacc gtgtaaaaaa caacctgcaa ataataatca gcctcataga attacaaact
     2461 cttcacactg acaatccatc gactataaca ctgttaaaag aaacatcggg gcgaatacga
     2521 tcgatgggta taatacatga aaagatatac cagacaaaag atctgacaga agtacccgca
     2581 aaagattatt tttcaactat cgttgatgaa ctgatgttca gctataatat tgaccacaat
     2641 aaaatcaaaa tagttaagaa catagacgac ataaccttta acatggatac agccatacct
     2701 cttggtctta tcatcaatga actactgaca aatactatta aacatgcttt taaaaaggat
     2761 caagagggtc gcattcatat atcggcaaaa aaatttgcta acagaactga aatcattgtt
     2821 tcagataatg gcataggaat accgataaaa gaagagatgg ataagcgttc ccgattgggt
     2881 atgcaattaa tccgaggttt aacaatgcag cttggagcgg aactttcgat tatcgcaaaa
     2941 aatggtacaa aaacaaaaat tacgctacaa aatacttgat aatatggcta attaaagtcg
     3001 caatgactta taaccttgaa atagatttta gatgttacag gaattatttt ttctatactt
     3061 taatgataac cgttaatact gatcatattg ataaagattg catatagaac tgttctgcaa
     3121 aatataaccg gtttttccct gtttaccaac atattaccgg tattatttgt ggacttacaa
     3181 tcaaattagt aatactttga agatgctcat tcataatagt gaagctcggg gatttttatg
     3241 aggataaatg ttaggggact caagttccaa atacttaaaa actatgtcat aatagctgct
     3301 attcctgtgc tttttacatt ttttataaca tcgggtttga tgaaaaacaa cacgcaaacc
     3361 cgttttcttg ataattcagc catgtctgta aatatttcag atctagctat tcagacaatg
     3421 atcaacagat ataaacatga gtttgagatg ctagcccgtt ctcaaaattt actttcaggt
     3481 tcgatataca cctttaacag cgataaaaaa acaccggtaa aagattccct gtcgggtaat
     3541 gccgcatcag acctaataaa tgatctaaaa catcttactg acgacactac tgagataaca
     3601 agggtttacg cggtaaaaac aaactcttca ctgtttcagt ttcctactca aaaaatggct
     3661 gctccttttg cagtgactga ctcaccactg tttaaacaag ctctctcatc aaacgaaacc
     3721 caaatatcgg agctctattt tgacagtgaa ctgaatacca cacttcttac ttttgcccgc
     3781 aaacttgata gtgaaaccgt tttttttgcg gatatcgatt ttcaaaaaat gacatccttt
     3841 atatcatcac ttaactatga aaaatccggc tatctcgtga tatgtgatga agatggaaaa
     3901 atcatcaata acccgcgtac tcctgagctt aaccaacaac caattacagc aatcggaatc
     3961 agcggtcttg aaacagtagc aaagaaaaca cacgatcaat tagcaggtct ggactcaaaa
     4021 aaaatcagtc atgttgtcaa cttctttact tccaaagagt actcatggaa atacgtcatc
     4081 cttgttaagt acgacgaagt tgttgacggg gtcgcttttg tttttaacat caccctcttt
     4141 ttcatattct taatggtcat gatctttatt ctttacggtg caaaaagtgc taaatatatc
     4201 attgaaccaa ttactacctt gcgtgaatat ctctggtctg cttcgaaagg tgattttcac
     4261 aacacgatgt ccaccaagat tacaaaacgg aaagatgtaa ttggcgaatt agctcagagt
     4321 tataacgatt ttatcacaat aacccgtgat gtaatcagtg atgttaacca ctctgccaat
     4381 cagcttgcct attccgctga cgaaatatca tcagctgtta aggatttttc ggcaaatatt
     4441 caaaaccagt cggcaaattc agaagagata tccgcctcag ttgaagagat ggaagcgagc
     4501 atgattaatg ttgccggtga gtcggaaact cagaacaaca gcataaagta tctcgacaaa
     4561 cagattaaag atatgtcggt aatgttacgc gaaatacaga agatgacaca aaacacattt
     4621 actttaaccg agaaactaca atcacgtgca gctacaggag aacaaacgct tcgtaccatg
     4681 gatcacacca tggttaaact gaacaactca agtcaagaca tggtaaatat tcttgcaata
     4741 attgacgaga tatccgaaca gataaatctt ctttctctta acgccgctat tgaggcggca
     4801 cgcgcaggtg atgcaggaaa gggattcgcg gttgtagcag atgagatatc taaacttgct
     4861 gatcagaccg ccaagagctt aaaggatatt gacagtctta taaggatgaa cagtagtgaa
     4921 atatccgacg gacaaaaaaa catcaacgag acgttactct tgattacgga ttttctaagc
     4981 ggtatcaatt cgataaatga tatgaaccgt aaaatcggaa actttatgac acagtactct
     5041 cagacaaacg atgaggtttc gaaccgagcc gaaaatgttc aaaaaatatc tgaggggatc
     5101 aagcacgctg ctgatgaaaa caaaagagca atttcggaaa tcgctagatc aatttttgat
     5161 ataaacgatc tttctcagaa aaatgctaca cactcacaag acgtttctga taatactaaa
     5221 aaacttgctg ccatgtcaga atcccttaaa tccaagatca agtttttcaa gatatagaac
     5281 gagaaagggg tcatcaatcc tcagatcaat gaccccttta aaaagttggt tctgtcaatt
     5341 ctgctttcaa aactttagca tacctcccaa cgtcagtagt tctttctgga tcttccaccg
     5401 gctaccgagc atggcagatt acctgccgtg ggtgaagctt cataatcacc ccgtttgagc
     5461 cctttttccc gttccagcaa ctacttataa tataataaaa attaaagaat cgtcaataga
     5521 gacataagtt tttttacttt ttttcaaata aataattaat cgataaagaa accatttcaa
     5581 tcatatacat tagcaagcct actaggtcaa ataaaatcaa tcttcaaagc aaagcctttc
     5641 aatcttgttc ttgacataat cactaagaaa agttactcaa tgacaatgaa cagagaaaac
     5701 tgcagggatt ttattaaact ctatttttct aaaccctatc tgcatgccga ttttgatata
     5761 attcacaaag gcatcgattg ctttaaacat attcccgaag atattgagcc tgaacagtac
     5821 atgaatcttt tacaggttct tatacaaaag aatgggattt tttcttatct cagatatttt
     5881 caggataatc acttcgcact gctctgctct gtattacact ctattacatt acaatgcatc
     5941 aaaaaaaacg acgaaaagca aaatgatcag acagaaaagg gttcagataa atttgttgta
     6001 cccgatatcg tagagctctc atttctcttt gattgtgatc tgcgcaccaa tcctgattta
     6061 atcttgaaat ataatcaaaa cagaattgta caatttcgca aggctcttga tatgcttctt
     6121 acagaaaaac gttacgtatc ggcaaagagc ttcaaggcta taacgctctt taagtacacg
     6181 gcagataaaa acatattaat acgagcaaaa aacattaccc gctcatttat taacgatttt
     6241 ccgctttctt cacaaaaact tatgctattc ttgattgcac taattgctct agatgagaac
     6301 ctacaggaaa cagagcatat cgaagaactt atgaaattat gtagtgatga tgattttaaa
     6361 agatctataa gttctgcaat ttttagactt aaaaatcaac gatttcatta taagcctttt
     6421 tagctttatg atccgtatca aatacaaacg gataattttt caagggttta ccagcattcc
     6481 aagttttgtt gtaatgataa tctaaccatg tatgatcatc agcaatcccc cagaaagtta
     6541 cagatgcgat cttgtccgaa tttattctga atagctcaaa tatttcccta tatcttgctg
     6601 ccagtttatc atctaaatct ttatcgaaag cccgtccctc tttattacct atcccatcaa
     6661 aacagtcaat atcaagctct gtaatatgga tatcaagccc catctctgaa aacatattta
     6721 ttgttctttg gatctcgtta atttctggcc aatagagatt ccagtgagcc tgaattccta
     6781 taccgtctaa accgtgtttc ttaagttcta actttttgat catggttatt atgtcatgcc
     6841 tttttgaagg gcgcactaca gcgtaatcat tatatataag tgaacaatca ggatcagccg
     6901 ctttggcgaa tttgaaagca tcaactatat actctgggcc accataggcc ttgtaccatg
     6961 gagaatcagc acgatagctc ccctggtcgg atactgcctc atttacgaca tcccagctgt
     7021 aaattttgcc tttgtagcgg cctaccactg tcataatatg attacgcata ttttccctaa
     7081 ccacatcctt gctaccttcg gtaatccagg gtgaagtctg actatgccaa acgagagcgt
     7141 gccctcgtac atttatatta ttagaggatg cataattaat aattctgtct gccgctttaa
     7201 agttaaattg atcttttagt ggatgtatca cttcaggttt catagcattt tcagcagtca
     7261 atgagttgta gtgaagtaaa agttctgcat atcttatgtc aaaatagtcc ctgttgccat
     7321 attcggaaga agctacggca gctccgatta gaaagtgctc tttataaacc tgactaatca
     7381 ttttttttgc tttaaacgtt ttcggcttgt ccgaaagatt aggactgctg cacccaataa
     7441 aaaaaagcaa tcctgacaaa agaattattt gttttattga aatcatcgtt attttctcct
     7501 aaaatattta taatcggaaa caaattttat ttaatggtta atcttacctg atttacggta
     7561 atcgttctta ttctaatatg gttttatctt gttttttttt gttatttata gaattattac
     7621 aaaatttaag agaataatca accgtattta aaaatacata atcacagatg cgattgtgtt
     7681 tattataaca acaaatgcct tgacataatt gtacatctat cataaaattt atttttgtgt
     7741 attgatacct gtctatttat tataaaagat aaccagttca attatcgata cattattgta
     7801 tttgaatata ttcctgtgta aaagcattaa tcctgtaaaa aaacgagaaa ctttcgttat
     7861 aaagagaaat gatatgagta aaatgtttaa atattacggt ataaacgttc aaaaaggaga
     7921 ctatcttttt aaagaagggg atgccgccga ctacctctat atgattcaca agggtgaaat
     7981 tctcattaat agaataataa acggggtaga agagaagata aaaattctcg aagagggaga
     8041 atttgtcgga gagatggcca taattgattc aaaaccgaga tcagcaaatg cgatcgcatt
     8101 gaatgattgc cagttgatac gcatgacaaa ggaagctttt gagataaatt atcagtcaaa
     8161 tccccaactt gggatgtctt ttttgaaatt tctatcttca cgaataaggg acacaaacga
     8221 aatagtctct tttatgcttt ccaaaaaccg tgatcaattc tttatgcttg agcttttcaa
     8281 actggttaca gtttctggca aaaaagatag tacgggcaac tggtttcttg ttgattattc
     8341 aaatttcgtc gagcatatga taaatcgatc taattataac gaaaccgaag taatttcgat
     8401 aatacaggaa atttgcaaaa aggacaacgt caagctggtc aactcaaaaa ataaaaatca
     8461 atggatagca acaaaagttt agtttaaaat atcccgtaac tcttttcaat tcaaccccag
     8521 agttttcttg gcaatggttt atcatacctt attaattttc actcaataag atttatgagt
     8581 ttatttggtt ggcggaaatc gcgtattctt ccagccgaaa cgacaattat cgaaacgcac
     8641 ggtattcacg cagatactgt taacggataa gacatcttga tagatccaga tgtcacatta
     8701 aaaaggttga ttgttcaata taataatcta ttagagctaa agcaatatta tcgcgggaga
     8761 ctaaggacgt gaatacattc agtaaatgaa gcttgacaaa gaaatagaag cattaataga
     8821 atcacattgg tcataaataa tttt
//
LOCUS       DAOTGZ010000109         8797 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_3064
            NODE_9537_length_8797_cov_5.628689, whole genome shotgun sequence.
ACCESSION   DAOTGZ010000109 DAOTGZ010000000
VERSION     DAOTGZ010000109.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868088
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 8797)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8797)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:22:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,785
            CDSs (total)                      :: 2,751
            Genes (coding)                    :: 2,732
            CDSs (with protein)               :: 2,732
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 12 of 19
            Pseudo Genes (internal stop)      :: 5 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8797
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9537_length_8797_cov_5.628689"
                     /isolate="AMR_MDS_3064"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..388
                     /locus_tag="P5123_08180"
     CDS             <1..388
                     /locus_tag="P5123_08180"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HRX16276.1"
                     /translation="EMAAIAAELIKQKVIAASFDQFCPETLYDGIWASASFLHIPYEL
                     LAESVNRYLKFLKPGGIFYLSFKKRDKDFSDGRRIFTCINEEKLSNFTDLLQDAIILD
                     SVTTNDLRPGRSEELWLNVFLKKKVV"
     gene            461..1120
                     /locus_tag="P5123_08185"
     CDS             461..1120
                     /locus_tag="P5123_08185"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013700.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M23 family metallopeptidase"
                     /protein_id="HRX16277.1"
                     /translation="MRKITKKIEFIDYYISVCKILLLSFLIVIISCATNSSPGESVEV
                     DICENYSDWQESEYILPYEPGSAYAVIQGNCAPKSITWAHYDNNRYAYDFGMPIGTPI
                     LAVRAGRVIFVRENFTDDQHEQEQGNALVVQHEDNTIALYGHMTQNGVLVGLGDHVEQ
                     GDVIALSGNSGMSPVPHLHLQVNDCADFNVCESVPVTFRNARPNNGRLLKDETYEAVA
                     E"
     gene            complement(1158..2573)
                     /locus_tag="P5123_08190"
     CDS             complement(1158..2573)
                     /locus_tag="P5123_08190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007220394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family transposase"
                     /protein_id="HRX16278.1"
                     /translation="MKREDAEKIYDLGKEAVVDLILQLVERIERLEEQLYKNSNNSSK
                     PPSSDGLKRKPRTRSQRKRTGKKTGGQKGHKGKTLKQVSSPDEIVPLLVERCSCCGEP
                     LDHHVFKGHDKRQVFEIPKISIHVTEYQAEIKDCPHCGKENKADFPEGVTHKVQYGNY
                     LKSIASYLRNYQLIPLERTKEIFEDIFSIPLSEGTLVNITNKCSESLNEFNRWVINKL
                     AHSRVVNFDETGVNCGGTLHWLHTAGTELLTSYFVHKKRGSKACDAMGILKEFKGTAV
                     HDHWQSYFKYSCDHSLCNAHHIRELTFVHEHHKQKWAQKMITCLCELKCEVENAGTKG
                     NLIEPYRMKYYESRYKRILREGFALNPPPEAENVKKRGRKKKGKVLCLLERLRVYRKE
                     TLAFMFNCDVPFDNNLAERDIRMVKVQQKVSGLFRTFSGAEQFCSIRSFISTVRKQEF
                     NILDALERIFNGEQVYLKFNC"
     gene            2708..3913
                     /locus_tag="P5123_08195"
     CDS             2708..3913
                     /locus_tag="P5123_08195"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRX16279.1"
                     /translation="MKRKMRKISNKKVLVFFSMALICFISCDSSSDDGNQVLTDIKVN
                     IDQPAGSMIPQSFTGSVEYSISDKVFDDNNSYKIQAYLKSADENYMFNNSVSLNSQQG
                     EEKFSFEITNDGLPADVTKPYNLFFSIVEINGATETVLETSENFVYKENSLTGSYEGH
                     LSSGYDMLVIFQHYGDLLVGTMYIDVDHDDDYYSAGDEAVGMTASVDGADVKMTLNGS
                     GIVFSTGKEYAGTAKVFGDNLKITCDYHRADNDAINGDFNLSRIGTGKIKGTIDASNA
                     AAPADNLFGKEYQVTLMFNLSAGEDGSYLTIEGSWPASGTTLDYEIDKVAEGSYQVVA
                     IIDNDGHFNGPETGDVFGIYSLTSEQTLTALSGWVSDPEYDLSYIDNVNVVAEETLPG
                     IDISVGLVP"
     gene            complement(4051..4539)
                     /locus_tag="P5123_08200"
     CDS             complement(4051..4539)
                     /locus_tag="P5123_08200"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRX16280.1"
                     /translation="MKNAIIGLYVLLVLVFSLALTGCSDEDSDSSGKKEVFELTGTWA
                     ADNVKMTTSDYSITVNIVTTFTENTFEGTTTITSNDPDMITTNSGELTSFDNSENWYV
                     GKLLNDSTGSDTIGKYGMTRYTLSDDGKSFVGTSYSYEDSEKEAKASTTIISENITMI
                     KQ"
     gene            5165..5725
                     /locus_tag="P5123_08205"
     CDS             5165..5725
                     /locus_tag="P5123_08205"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRX16281.1"
                     /translation="MKNLMKMNWSRNIASLFAISMLVISLISCSSGGGSGSSDSDPDA
                     PSEVSVEYKRSGSSSIEVSWLGDSKSGSYSVVVDKSSPYDGDPALIQEKASSPAKFVN
                     VSSLPNGDYYVYVVPVNADGVEGEAVAAKSVVTIGAAVSPSGDGTGSPVNPAPTRPIV
                     PVVTNPPNYDYPVDPDLDSDVDGVPD"
     gene            5735..8635
                     /locus_tag="P5123_08210"
     CDS             5735..8635
                     /locus_tag="P5123_08210"
                     /inference="COORDINATES: protein motif:HMM:NF024777.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ig-like domain-containing protein"
                     /protein_id="HRX16282.1"
                     /translation="MAGTDPNDEDDYPETSDPDADWGSVTPDTDSDGDGVPDWIEIVL
                     GTDPFDSTDKPADGTWEDLVDLDPFADDDEDGVPNWVEVAHDTDPADPTSRPQDSDLY
                     GDPEGDADGDGIPNWTEIISGSDPFNPGSYPDVPEGEDWSDIEYDTDSDGDGVPDWIE
                     IVLGTDPFDPDSTPAEGTWEHLVGLDPLGDADNDGVPNWIEIVYGSNPNDASSTPSPE
                     QLTGDPWGDADGDGVPNWIEIVLGTDPFNPADIPADGSWEHLLTGDPNADTDSDGVPD
                     WIEIIFGTDPTDGSDRPTNDMVIDDPRGDADGDGVPNWIEIIEGTDPFDPTDYPADGT
                     WEDHLNEDPNTDTDGDGVADWIEIIVGTDPGSDESTPVYVEDDEGNGTYLDPDGDADG
                     DGVSNGDEADYGTDPTNPEDYPTGADAPTNVSAAIGGAYNQITVSWSGAINNGSYTVE
                     IRNNINPSAGLVDIKQNTESPVTFTELARGDYYVTVYPVNYDGTIGEGVSAGAAISVV
                     NIDLDLQEAYDALVPGFQSPDSAESVTKNFTLPVNGLNDTQISWVSSDESTVILSGSG
                     ASVSRPANGLGNKSLQLTATITKDGQSVQKIFVITVIEMPIEVFSELIKPGNGAIQVE
                     LDKTIEIPFACDLAGSSVDSDSVYVTDSNGNPIAMTVSYDDTDEIVTKGDVDYDVKGR
                     IILVPQASFEPETSFTVTVTTDVKKDDMTSLFSAIEFSFTTMELVDYHYTFEDLSGFI
                     IPNGSGRNDVAIDYDNYHQGTSSIKFDGDCDHLSLGDVDLGDQFSVAIWVYLPSPGFA
                     IEEGTEGSAKGQMINTILSNAESTEPSDGFKLFVNSWGITDRRVIFEAGNGRNGGKLR
                     SPAGFVELDGWYHFVFTVNRDTQNKTVDVRLYFNGEAQPLFFVVDDTWQTGPLPNEIP
                     WDFNTSGELLVGQFTGGEGLWDRHNYYGNMDDLRIYNRVLSPAEVARIAQEN"
BASE COUNT         2546 a         1642 c         1913 g         2696 t
ORIGIN      
        1 cgaaatggct gcgattgccg cggaacttat taaacaaaaa gttattgcgg catcgtttga
       61 tcagttctgt cctgaaacgt tatatgatgg aatatgggcc agcgcatctt ttttacacat
      121 accttacgaa cttcttgcag aatctgttaa ccgttattta aaatttctaa agcccggtgg
      181 aatcttttat ctctcattta aaaagcggga taaagatttc agtgacggca gacgcatctt
      241 tacgtgtata aatgaagaaa aactttcgaa ttttacagat ttgctgcaag atgcaataat
      301 actggattca gttacaacca atgatcttcg tcccgggcgg tcggaagagc tttggctgaa
      361 tgttttttta aaaaagaagg tcgtttagat ttgttttgat ggttatacaa aggatgtttt
      421 tgattatcat aaaaatactt taaacacagg agaatactta atgaggaaaa taaccaaaaa
      481 aatagaattt attgattatt atatatcagt atgcaaaata ttattattat ctttcttaat
      541 tgtaataatt tcatgtgcta ctaattcaag tccaggcgaa tccgttgaag tcgatatttg
      601 cgaaaattat tcagactggc aagaatctga atatattttg ccatacgaac caggaagcgc
      661 ctatgctgtt attcagggaa actgcgctcc caaatcaatt acctgggcgc attatgataa
      721 taacagatac gcgtatgatt tcggaatgcc cattggtaca cctattctgg ctgttcgcgc
      781 gggaagagta atttttgtca gggaaaattt tacagatgat caacacgaac aggagcaggg
      841 aaatgcatta gttgtgcagc acgaagacaa tacaattgcg ctgtacggtc atatgactca
      901 aaacggggtg ttagtcgggc ttggcgacca cgtagagcag ggcgatgtta tcgcattgag
      961 cggtaattcc ggaatgagtc cggttccgca tctgcatctt caggttaatg attgcgctga
     1021 ttttaatgta tgcgaatccg tgccggtaac atttcgtaat gctcgtccaa ataatggacg
     1081 tctgttgaaa gatgaaacat atgaagctgt tgccgagtaa tatagtaata taaatgtcta
     1141 gtaactactc agctatttta acagttaaat ttaagataca cctgctcacc attaaaaatt
     1201 ctttcaagcg catccagaat gttgaattcc tgttttctaa cggttgaaat gaaacttcta
     1261 atagagcaaa attgttcagc tcctgaaaat gtcctaaaca atccggacac cttttgttga
     1321 actttaacca tgcgaatatc tctttcggcc agattattat caaaaggaac atcacaatta
     1381 aacatgaatg ctaaggtttc tttccggtaa acccgtaacc tttctaaaag gcataatact
     1441 ttacctttct ttttccttcc ccgctttttc acattttctg cttctggggg aggattgaga
     1501 gcaaatcctt ctctcagaat tcttttatac ctgctttcat agtacttcat gcggtatggt
     1561 tcgataagat tgccttttgt gcctgcattc tcaacttcac atttgagttc acacaagcag
     1621 gttatcattt tttgtgccca tttctgttta tggtgctcat gtacaaaggt taattccctt
     1681 atatgatgag cattgcacaa tgaatgatca caactatact taaaatatga ttgccaatgg
     1741 tcatgaaccg ccgtgccttt gaactctttc aatattccca tggcgtcaca tgccttcgaa
     1801 cctcttttct tatgaacaaa atacgatgtc aataattcag ttccggctgt atgaagccag
     1861 tgcaatgttc ctccacaatt tactccagtt tcatcaaaat taactacccg tgaatgagca
     1921 agtttgttga tgacccatct attaaattca ttaagagatt cagaacattt gtttgtaata
     1981 ttaacaagtg ttccttcaga cagtgggata gaaaatatat cctcaaaaat ctcttttgtt
     2041 cgttctaatg gtatcagttg atagtttctc aaatatgacg cgatactttt caaataattt
     2101 ccatattgga ctttgtgcgt tactccttct ggaaaatctg ctttgttttc ctttccacaa
     2161 tgaggacaat ctttaatctc tgcctgatat tcagtaacgt gtattgatat ctttggtatc
     2221 tcaaatacct gccgtttgtc atgacccttg aaaacatgat gatccagtgg ttctccacaa
     2281 cagctgcatc tttcaactaa tagtgggact atttcatctg gggaactcac ctgcttcagg
     2341 gtcttgcctt tatgcccctt ttgtcctccg gtcttcttgc cggtacgctt tctttgactt
     2401 cgagttctgg gttttctttt caacccgtca cttgaaggcg gtttactgct attattgctg
     2461 tttttgtata attgttcttc aagcctttca attctttcaa caagttgaag aattaagtca
     2521 acaacagctt ctttacctaa atcgtatatc ttttcagcat cttctctttt cataagacat
     2581 tatgttactt atgtctctga aattaatagt aaaaaaaata tctgttgaaa ttttttagct
     2641 gagtagttac aatgtctatt aaggatttta taaaaaaaat aatttagatt attagcaggg
     2701 aggagttatg aaaaggaaga tgaggaaaat aagtaataaa aaagttttgg tttttttttc
     2761 catggctttg atttgtttta taagttgtga cagttccagt gatgacggga atcaggtttt
     2821 aactgatatt aaagtaaaca tcgatcaacc ggcagggagt atgattcctc aatcttttac
     2881 aggttcagtt gaatatagca tttctgataa ggtttttgat gataataaca gttataaaat
     2941 tcaggcgtat ttgaaatctg cagatgagaa ttatatgttt aataattcag tttcattaaa
     3001 tagtcagcag ggtgaagaga agttttcttt tgaaattacc aacgatggtt tgccggctga
     3061 tgttacaaaa ccttataatc tgttttttag tatcgtggaa attaatggcg caactgaaac
     3121 tgttttagag acttctgaaa attttgttta taaagaaaat tctttgaccg ggtcatatga
     3181 agggcacttg agttccggtt atgatatgct ggtaattttt cagcattatg gtgatttgct
     3241 ggttggcact atgtatatag acgttgatca tgatgatgac tattatagcg caggtgatga
     3301 ggcggttgga atgactgctt ctgtggatgg tgctgatgta aaaatgactc tgaacggcag
     3361 cggaatcgta ttcagtacag gtaaagaata tgccggaaca gcaaaagtat ttggtgataa
     3421 tttaaaaatc acctgtgatt atcacagagc agataacgac gcaataaatg gagactttaa
     3481 tctttcccgg ataggtactg gaaaaatcaa gggaacaata gatgcttcaa atgctgcagc
     3541 acctgcggat aatctatttg gaaaagagta ccaggttacg ctaatgttta acctgtcggc
     3601 tggtgaagac gggtcttacc tgactattga agggagttgg cctgcttcag gaacaactct
     3661 ggattatgaa atagataaag ttgcagaagg cagttatcaa gtagttgcaa taattgacaa
     3721 tgatggacat tttaacggac cggaaactgg cgacgtgttc ggaatttaca gtctcacttc
     3781 tgaacaaaca ttgacagctt tatcaggctg ggtttctgat cctgaatatg atttaagtta
     3841 tattgataac gttaatgttg ttgcggaaga aactcttcca ggtattgata tttctgttgg
     3901 gttggtgccg taaagaaata agattattaa tatccaggat cccgataaat tatatcggga
     3961 tcctgatttt tcattaaata tgatttaaag aattttatga gtgaacaaaa ttggtgagcc
     4021 ttgttaataa tattatgtaa atttaacatc ctattgcttt atcattgtga tgttctcaga
     4081 aattatagtt gttgaggctt ttgcctcttt ctcgctatct tcataagaat atgaagtccc
     4141 aacaaaactt ttgccgtcat ctgacagtgt atatctagtc attccatatt ttccaatagt
     4201 atcagaacca gtactgtcat tcaatagttt accaacatac caattttctg aattatcaaa
     4261 gcttgtaagt tcaccactat ttgttgttat catatcgggg tcattgcttg tgatagttgt
     4321 tgtgccttca aaagtgtttt cagtaaatgt tgtgactata tttacggtaa ttgaataatc
     4381 gcttgttgtc attttaacat tatctgctgc ccaagttcct gttaattcaa aaacttcttt
     4441 ttttcccgat gagtcggaat cttcatcgga acatcctgtt aatgctaaac tgaaaacaag
     4501 tacgagcaaa acatataaac caataattgc atttttcatt ttaaccacct gtattattat
     4561 ttaaaaaata taatttcagc tgaaatggtt acagtcaagt gtattatcgg gttttagaga
     4621 gataaaagcc tgatgttata aaaaaccttc atgcggctat tgagtttatc aatttttatc
     4681 attcttttaa aatttcccgg taatgaatct ttgctgacga tagcagagta gtgattttat
     4741 ctttagttgc gtcggatgct ttaataatcc tgccgctatg gcggattacc tggttgaccc
     4801 cggcccggac attggagcaa gtgcttttct taaccatgtc taataacatc tatacatatt
     4861 catatacaga aaattgttgt ccgttttgat taaaagccaa aaaaaagttt ctgttataac
     4921 aagaaaatag tgcacgcacg taaatttgct gggtcgccgt tcctgaagga caaatggact
     4981 attattagcc ctgttaataa aaaacagggg atgttctatc acatctttac ctcaaaaacc
     5041 aaaaaaaatt acattttagt ggaaaaatca cattaatcat tgacattaat cacataataa
     5101 ccgaaaataa acccatacgg gcggtctaaa tgcccgattt cttgttacgt gaaaagggga
     5161 ctttatgaaa aacctgatga aaatgaactg gagcaggaat attgcatctc tgtttgcaat
     5221 ttctatgctc gtaatttcgc tcatcagctg ttcaagcgga ggaggaagcg gctcctccga
     5281 cagtgatcct gatgctccat cggaggtgtc agtggagtac aaacgaagcg gctcttcttc
     5341 tatagaagta agctggcttg gtgattcaaa aagcggaagc tatagtgtgg ttgttgacaa
     5401 aagcagccct tatgacggcg atcccgcatt aattcaggag aaggcctctt ctccggcaaa
     5461 atttgtcaat gtttcttctt tgcctaacgg tgattattat gtatatgttg tgcctgttaa
     5521 cgcggacggt gtagagggcg aggctgtcgc ggctaaaagc gttgtgacaa taggagctgc
     5581 ggtatcaccc agtggagacg gtactggctc gcctgtgaat cctgcaccaa caagaccaat
     5641 cgttccggtt gtgaccaatc cgccaaatta tgattatccg gtagatcccg atcttgacag
     5701 cgatgttgat ggagtgcctg actgaataga gattatcgct ggtacagatc caaacgatga
     5761 agacgattac cctgagactt ctgatcctga tgccgactgg ggttctgtta ctccagatac
     5821 cgacagtgat ggagatggag ttccagactg gatagagata gttttgggaa ccgacccgtt
     5881 tgattcaact gataaaccgg cagatggcac atgggaagat cttgttgatc ttgatccttt
     5941 tgctgatgat gatgaagacg gtgtgccaaa ttgggtagaa gtagctcacg atacagatcc
     6001 agctgatcca acatcgcgac cacaggatag cgatctttat ggcgatcccg agggtgacgc
     6061 tgacggtgac ggaataccta actggaccga gataataagc ggcagtgatc cgtttaaccc
     6121 gggttcttat cctgacgtac ccgaaggtga agactggtcg gatattgaat atgataccga
     6181 tagcgatggt gacggagtac ctgactggat agagatagtt cttggaacag atccttttga
     6241 tccagatagc actccagctg agggtacatg ggagcatctt gttggccttg atcctctggg
     6301 cgatgcagat aatgatggtg tgccaaactg gatcgagata gtatacggtt ctaatccaaa
     6361 tgacgcaagt tctacccctt caccagaaca gttaaccggt gatccatggg gagatgcaga
     6421 tggtgacgga gtaccaaact ggatagagat agtgcttggt acagatcctt ttaatccggc
     6481 tgatataccg gccgacggca gctgggagca tcttttgact ggtgatccaa acgccgatac
     6541 agacagtgac ggagttccag actggataga gataattttt ggtacagatc caaccgatgg
     6601 ctcagatcgt ccgactaacg acatggtgat cgatgatcca cggggagatg ccgatggcga
     6661 cggagtacca aactggatag aaattataga gggaacagat ccttttgatc cgactgatta
     6721 tcctgctgac ggaacatggg aagatcatct gaatgaagat ccgaacacag acacagacgg
     6781 cgatggcgtt gctgactgga tagagataat agtcggtact gatccgggca gtgatgagag
     6841 cactcctgtt tatgtcgagg atgatgaggg taacggaact tatcttgatc ctgatgggga
     6901 tgctgatggt gacggagtct caaacggaga cgaagccgat tatggcacag acccgacaaa
     6961 tccggaagat tatccgacag gtgctgacgc tccgacaaac gtttcggctg ctatcggcgg
     7021 tgcttataac cagataactg taagctggag cggcgcaatc aataacggta gttatacggt
     7081 tgagataaga aacaatatca atccaagcgc tggactcgtg gatatcaaac agaatacaga
     7141 gtcgcctgta acctttaccg agcttgctcg tggtgactat tatgttactg tttaccctgt
     7201 aaactacgat ggaacaattg gcgagggagt aagtgccggt gctgcgatat cggttgtaaa
     7261 tatcgatctt gaccttcaag aggcttatga tgctttagtg ccgggttttc agtcgcctga
     7321 cagtgcagag tcagtgacca aaaactttac actacctgtt aacggtctta acgatacgca
     7381 gataagctgg gtgtcgtcgg atgagtctac tgttatactc tcgggttctg gcgcaagtgt
     7441 ttcgcgtccg gctaacggtc tgggtaataa gtcattgcag ctaacagcga cgattaccaa
     7501 agacggtcaa agtgtacaga agatctttgt tattacagtg atcgagatgc caatagaggt
     7561 cttctctgaa ctgataaaac ccggtaacgg agcaattcag gtagagcttg acaagacaat
     7621 agagatacca tttgcctgtg atcttgccgg ttcttctgtt gactcggatt cggtttacgt
     7681 aaccgatagc aatggcaacc cgatagccat gactgtgtct tatgatgata cagacgaaat
     7741 agtaaccaaa ggtgatgtcg attatgatgt taaaggtagg ataatacttg tgccacaggc
     7801 atcgtttgag cctgagactt cgtttactgt tactgtaact accgatgtta aaaaagacga
     7861 catgacatcg ctattttcag cgatcgagtt ttcgtttacc accatggagc tggtagatta
     7921 tcactatacc tttgaagatc ttagtggttt tataatacct aacgggtcag gacgcaacga
     7981 tgttgccata gactacgata actatcatca gggtacatcg agcatcaagt ttgacggcga
     8041 ctgtgatcat ctttcacttg gagatgttga tcttggtgat cagttcagtg tagctatctg
     8101 ggtttatctt ccatcaccgg gttttgcgat cgaagagggt actgagggat cagcaaaggg
     8161 tcagatgatc aacacgattc tttctaatgc cgaatctact gaaccaagcg acgggtttaa
     8221 actctttgtt aatagctggg gaataactga ccgtcgtgtt atttttgagg caggtaacgg
     8281 aagaaacggt ggcaagcttc gaagccccgc gggttttgta gagcttgacg gttggtatca
     8341 ctttgtcttt acggttaaca gagacactca aaacaagact gtcgatgtgc gtctctattt
     8401 taatggtgaa gcacagccgc tgttctttgt tgtcgatgac acttggcaaa caggaccgct
     8461 tccgaatgag attccatggg attttaatac cagcggagag ttacttgtag gccagtttac
     8521 aggcggagaa ggactttggg acagacacaa ctactacggc aatatggatg acctccgtat
     8581 ttacaaccgg gttctatcac ctgcggaagt agcgcgtata gctcaggaaa attgataata
     8641 tagtccaaac aaatacgcag tcttcaagcg tatttgtttg gacggtttcg tttttctctt
     8701 ccggagaggt tttttttgtt tttaacaaaa caagttttct gtcctttctt tttcaatagc
     8761 aaaaaagaaa gaaccacata gtcaaaacaa ttgcgca
//
LOCUS       DAOTGZ010000110         8690 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_3064
            NODE_9712_length_8690_cov_4.591778, whole genome shotgun sequence.
ACCESSION   DAOTGZ010000110 DAOTGZ010000000
VERSION     DAOTGZ010000110.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868088
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 8690)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8690)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:22:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,785
            CDSs (total)                      :: 2,751
            Genes (coding)                    :: 2,732
            CDSs (with protein)               :: 2,732
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 12 of 19
            Pseudo Genes (internal stop)      :: 5 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8690
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9712_length_8690_cov_4.591778"
                     /isolate="AMR_MDS_3064"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..325
                     /locus_tag="P5123_08215"
     CDS             <1..325
                     /locus_tag="P5123_08215"
                     /EC_number="2.5.1.75"
                     /inference="COORDINATES: protein motif:HMM:NF013845.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="tRNA dimethylallyltransferase"
                     /protein_id="HRX16283.1"
                     /translation="TDILKKRISHRLDERLAQGMVDEVRTLLESGVSRERMIMLGMEY
                     KYITLFLNGDINFETMKDHLLHEIFRLAKRQRTWFRGMKKRGLDITWVESITAERLAE
                     LFNAS"
     gene            406..798
                     /locus_tag="P5123_08220"
     CDS             406..798
                     /locus_tag="P5123_08220"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRX16284.1"
                     /translation="MKKAVSFYLFCILLILLSSCSTFIKEEQVESVMKREAYLYETLI
                     DVKYEEQVVIKKGTEVQIYFVSSKNYLKVYAFPNDKGYLASEHYLILYLFGDDFESEI
                     FEEDKFYPMLDEVVQRKRELTAEERERR"
     gene            835..1713
                     /gene="dapA"
                     /locus_tag="P5123_08225"
     CDS             835..1713
                     /gene="dapA"
                     /locus_tag="P5123_08225"
                     /EC_number="4.3.3.7"
                     /inference="COORDINATES: protein motif:HMM:TIGR00674.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008840 - 4-hydroxy-tetrahydrodipicolinate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate synthase"
                     /protein_id="HRX16285.1"
                     /translation="MFKGVYTALVTPFKDGSIDYDSLDAIIEEQVAAGVSGVLPMGTT
                     GESPTVSHEEHNQIIAHVVKAVNGRVKVLAGTGSNSTDEAVFLSKNAEKCGADAVLVV
                     NPYYNKPPQRCLVDHFATVAKNIGVPVMLYNIPGRTGVNFEPESMAELCEISSNVVAL
                     KEAAGDIGQVMRIVELLGNRLNLMIGDDNLVLPAMAVGATGVVSVLSNIIPADMVRVA
                     SSWLNGEHAESKELFYRILPLCRMMFLDTNPIPIKAVMAAAGKCSPQVRKPLLPLEDD
                     KLKMIIQTFAEYGVKL"
     gene            1710..2510
                     /gene="dapB"
                     /locus_tag="P5123_08230"
     CDS             1710..2510
                     /gene="dapB"
                     /locus_tag="P5123_08230"
                     /EC_number="1.17.1.8"
                     /inference="COORDINATES: protein motif:HMM:TIGR00036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008839 - 4-hydroxy-tetrahydrodipicolinate
                     reductase [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate reductase"
                     /protein_id="HRX16286.1"
                     /translation="MSVVSICGFGGRMGQAIARSLYSKGHSVGNSFDIPSSPFYGKDP
                     ALDIVGKNSGAIVGEISASAIKDVDCVVDFSAPTAAMKILDAAVEASKPIVIATTGLD
                     SDMEKRIEDASKKIAIVYATNMSVGVNLLFKLAEMAASVLGGSYDAEVFEAHHRNKKD
                     SPSGTASTIINHIRKGHRDFEKCTEAYRDRGIIGERTDNEIGVQVLRGGDIVGEHTAF
                     FCGLGERIEITHRATDRRVLADGAVRASEFVVSAKPGLYSMLDVLNIG"
     gene            2514..3473
                     /locus_tag="P5123_08235"
     CDS             2514..3473
                     /locus_tag="P5123_08235"
                     /inference="COORDINATES: protein motif:HMM:NF014950.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HRX16287.1"
                     /translation="MKGNNPHTASEYSGQDFKELLEYESNPFDTTKKRAADIMRIFFE
                     RNVPLLPVVSRDNHLFGIITKPALTAAMSDIDNFDSVKIDAFVMKVAQKMEFEEVVAL
                     IGNEKLFPVINIFGEEKGNWSRIDLLEASDVTRMKKNDVDLEVQQNKEDAALEWMIYM
                     ILEHIPRPLYAVNQNGGTIFYNGLFEQLVCERLDVFEPDVEYIEKSFADVTLNDYFEG
                     SLGEYCFYNKELDLYYEKVPMKNEGKSVGYLIYCSRGLKEHADAGTNQDAVSFDEKVE
                     AYERKLIVDALHASGNELTITAEMLDISRSALQKKIKKLGINT"
     gene            3738..5021
                     /gene="rho"
                     /locus_tag="P5123_08240"
                     /pseudo
     CDS             3738..5021
                     /gene="rho"
                     /locus_tag="P5123_08240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004765954.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0008186 - ATP-dependent activity, acting
                     on RNA [Evidence IEA];
                     GO_process: GO:0006353 - DNA-templated transcription
                     termination [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination factor Rho"
     gene            5032..5232
                     /gene="rpmE"
                     /locus_tag="P5123_08245"
     CDS             5032..5232
                     /gene="rpmE"
                     /locus_tag="P5123_08245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012388038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L31"
                     /protein_id="HRX16288.1"
                     /translation="MKSDIHPVYEETTIKCACGFEVKSRSTKKDVHVEICSACHPFYT
                     KEQRLVTTTGRVEKFKQKYKLK"
     gene            5654..6433
                     /locus_tag="P5123_08250"
     CDS             5654..6433
                     /locus_tag="P5123_08250"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor RpoD/SigA"
                     /protein_id="HRX16289.1"
                     /translation="MSAIIDRSICVAEKNDIDFKPTPVSDEFAKAYRGLVSSIAKNYA
                     SYQCPFDDLVQEGFMGLIRAFEKFDPSLGCKYTTYATYWIKHYITRYIAKHGRIVRLP
                     IKKSELYRKICKVRDYLSHEMGETPTLDQIAEYAEVSMEDVKRIMDIFQPELSFETPF
                     RDSTSDRYDVVEDEVNDGPEHAYFNEELKRELKHALSQLMDKERMIITLRFGLDTGVS
                     VTLKDVADSFGISSETVRQIEKRALVKIKTRFAYLKEYLYN"
     gene            complement(6483..7430)
                     /locus_tag="P5123_08255"
     CDS             complement(6483..7430)
                     /locus_tag="P5123_08255"
                     /inference="COORDINATES: protein motif:HMM:NF014533.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009294 - DNA-mediated transformation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-processing protein DprA"
                     /protein_id="HRX16290.1"
                     /translation="MNKYNYWIALSEAPGIGIASMKEIYKSLLSTGLSIQDIMELTES
                     EIEDEFSFSKRTVQGILKGQQLLENTEDVYQQILTAGIEPVLFFEENYPEQLKNSNSI
                     NTPPLLYCFGNKRNLQLPLITVMSGAEISSTADTIAYHTGKILAEHGICAGTGLNRGA
                     GTLFASGTLENRGELLAIIPCGLLTFNMSDNLKEIFDPDTCTIVSPFFPKEEISKHNA
                     IYRNELLSELSDAIYIIEMKKDDPVITPTVKHISKISTPLYATHYSEYPEEASGNQNL
                     FENYGALPIRGKKVGNIIQPNLDAIMAHAKFSKRTKEAN"
     gene            7856..>8690
                     /locus_tag="P5123_08260"
     CDS             7856..>8690
                     /locus_tag="P5123_08260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014956732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HRX16291.1"
                     /translation="MIEVNNLSRYYGDFCAVNNISFTINKGEITGLLGPNGAGKTSTM
                     RMITGFLKASSGDILVGDVNVNDDPTAVKKMIGYLPESAPLYGDMMVYDYLVYAAKIH
                     DIVEPETRINETAGLCGLIPVMHKNINELSKGYRQRVGLAHAMIHNPEILILDEPTNG
                     LDPNQIIEIRNLIKEIGKEKTVILSTHILPEVEATCDRIIIINQGKIVADNTTDQIQS
                     ASENSLAVKVSLTGASVADVKDNFKSINGILSVREAFEKDGLVSVILDTVSSRDLRPE
                     VF"
BASE COUNT         2691 a         1435 c         1910 g         2654 t
ORIGIN      
        1 gacagatata ctaaaaaaac gaataagtca caggttggat gagcgattgg ctcagggtat
       61 ggtggatgag gttcgcacat tgctagagag tggtgtaagc cgtgaaagaa tgattatgct
      121 gggaatggaa tataaatata ttacattgtt tcttaatgga gatattaatt ttgaaacaat
      181 gaaagatcat cttcttcatg agattttcag gcttgcgaaa cgtcaaagaa cctggtttag
      241 gggtatgaag aagcgtgggc ttgatataac atgggttgaa agtatcactg ccgaaagatt
      301 ggcggagctt tttaatgcgt cataatattt tcttgacttt ataattgctc aaaaatagcc
      361 taaacggata acagctctat aaaatgctga caacctggag taaatatgaa aaaagctgta
      421 agtttttacc tcttttgtat tttgcttatt cttttaagca gctgttcgac ttttattaaa
      481 gaagagcagg tcgagtctgt aatgaagagg gaagcctatc tatacgaaac attaatagat
      541 gttaaatatg aagaacaggt agtaataaaa aagggaaccg aagttcagat ctattttgtt
      601 tcctctaaga attaccttaa agtatatgcc ttcccgaacg acaaggggta tttggcgtcc
      661 gaacactatc tgatactcta cctgtttgga gatgattttg aaagtgaaat tttcgaagag
      721 gacaagtttt atccgatgct tgacgaggtt gtacagcgaa agcgtgaatt aactgccgaa
      781 gagcgtgaaa gaagataaaa cgggctaaga cccgaaaaaa atagaggaga tcgaatgttt
      841 aaaggagttt atacagcctt ggttacaccg tttaaagacg gttcaattga ttatgattca
      901 ctggatgcta ttattgaaga gcaggtcgca gccggagtat ccggtgtttt gccaatgggt
      961 actacggggg aatctcctac tgtatctcat gaagaacata atcagataat tgctcatgtc
     1021 gttaaagcgg tcaatggaag agtaaaggtt cttgccggta caggttccaa ttctacagat
     1081 gaagcagttt ttctctcaaa gaatgcagag aaatgtggtg cggacgcagt cttggtcgtt
     1141 aatccctact ataacaagcc tccccaaaga tgtcttgtgg atcattttgc tacagttgcc
     1201 aagaatattg gtgtgccagt tatgttatat aatattcccg ggcgaaccgg cgtaaatttc
     1261 gaaccagaat ctatggcaga actttgtgaa atttcttcga atgtagtagc tttaaaagaa
     1321 gctgcaggtg atattgggca ggttatgcgg atagttgaat tgcttggtaa ccgtctaaac
     1381 cttatgatcg gtgacgataa tcttgttttg ccggccatgg cagtaggcgc aaccggtgtt
     1441 gtttcggttt tatcgaatat tattccggcg gatatggtaa gggttgcctc aagctggtta
     1501 aacggagagc atgcggagtc taaagaactt ttttaccgaa tactccccct ttgcaggatg
     1561 atgtttctgg ataccaatcc aattccgata aaagcagtta tggctgcagc gggcaaatgt
     1621 tctccacagg taagaaaacc tcttcttcct ttggaagatg ataaactgaa aatgattata
     1681 caaaccttcg ctgaatatgg ggttaaatta tgagtgtagt aagtatttgt ggttttggcg
     1741 gaagaatggg gcaggctatt gcgcgttccc tgtattctaa aggtcatagc gtaggcaact
     1801 ccttcgatat accgtcttca ccattttatg gaaaagatcc tgcacttgac attgttggca
     1861 agaattcggg tgcaattgtt ggagagatat cggcatctgc aattaaggat gtagattgtg
     1921 ttgttgattt ttcagcaccg acagccgcta tgaagatact tgacgcagca gttgaggcct
     1981 caaagcccat tgttattgct actactggtc ttgattctga tatggaaaaa cgaatagaag
     2041 acgcttctaa aaagatagct attgtatacg caacaaatat gtctgtaggg gtaaatcttc
     2101 tttttaaact tgcagaaatg gccgctagcg ttttgggtgg ctcgtacgat gcggaagttt
     2161 ttgaggcaca tcatcgcaac aaaaaggatt cgccatccgg tacggcatct acaataataa
     2221 atcatatacg taaaggtcat cgtgattttg agaaatgtac ggaagcttat cgcgaccgtg
     2281 gaataatcgg agaacgaacc gataacgaaa taggtgttca ggttttgcgt ggtggtgata
     2341 tagttggtga acatacagct tttttttgtg gtttgggtga acgaatagag ataacacata
     2401 gagcaactga tcggagagtt cttgcggatg gcgcggttag ggcttctgaa tttgtggtta
     2461 gtgccaagcc cggcctctat tcgatgcttg atgtacttaa tataggttaa aagatgaaag
     2521 gcaacaatcc gcatacagct tctgaatatt cagggcagga ttttaaagaa ttactcgaat
     2581 atgagtcaaa tccgtttgat acaacaaaga aacgggcggc tgatattatg cggatttttt
     2641 ttgaacgtaa tgtgcccctt ttgcctgtag tttcgagaga caatcatctg tttggaataa
     2701 ttactaaacc tgccttgact gccgcaatga gcgatattga taactttgat tctgtaaaga
     2761 tagacgcctt tgtaatgaaa gtagcacaaa agatggaatt cgaggaagtt gtagcattga
     2821 tcggtaatga aaaactcttt cctgtaatta atatttttgg agaagagaag gggaattggt
     2881 cacgtattga tcttctagag gcttctgacg taactcgaat gaaaaaaaat gatgtagatc
     2941 ttgaagtgca gcaaaataag gaagatgctg cacttgagtg gatgatttac atgatacttg
     3001 agcatattcc gcgccccctt tatgccgtta atcagaatgg aggcactatt ttttataatg
     3061 ggctttttga acagttggta tgtgagcgac tggatgtttt tgaacccgat gttgaatata
     3121 tagaaaaatc atttgccgat gttactctta atgattactt tgagggctct ctcggcgaat
     3181 actgttttta taacaaagag cttgatctct attatgagaa agttccaatg aaaaatgagg
     3241 gtaaatccgt tggttattta atatattgct cacgaggtct taaagagcac gccgatgccg
     3301 gtacaaatca ggatgctgtc tcttttgacg agaaggtgga ggcatatgag cgtaagctta
     3361 tcgttgatgc ccttcatgcg tccggtaatg aactgactat tacagcagag atgcttgata
     3421 tttccaggtc ggcattacag aaaaaaatta aaaaattagg aattaataca taaaacgctt
     3481 gaaaaaagtt tgacaattgc ccattgcatt tttatatatg ctctgaccag tccaaaaaac
     3541 cgctataata agtaaacaaa cattccgcaa acttcgagca tttctatatt tgatgtatgt
     3601 cggaaaatca tgataaaggt acgccgatgg ataatgattt aaagagtgaa gagactcttc
     3661 cttccgagca gggattgtca gatgcccctg cttcaacaga aaaagagtct aaacccgaga
     3721 ggaaggcatc tccttcccga agaagaaccg ggaaaatgcc tgtgcccgaa aatcttgatt
     3781 tggcagaact gaaactaaaa accatcacta atcttgtaac tattgccaaa gatatgggac
     3841 ttgaaggaat ttcaggacta aagaaacagg atctaatttt tcagatatta aaaggacaaa
     3901 ctgagcgtaa cggaaatatt ttcgcgacag gagttcttga ggttttaccg gatggttatg
     3961 gttttttacg ttcttcaaac tacaactatc tgcctgggcc tgatgatatt tatgtttcgc
     4021 cttcgcagat tcgtctattt gcgttaaaga ctggtgatac cattgcggga cagattaggc
     4081 ctcctaaaga taatgagcgt ttttttgctc ttcttcgagt tgaagcagtc aacttcgaaa
     4141 atccggaaaa ccttaccaga cgtattttgt ttgataacct tactcctctt catccgcatg
     4201 aaagattaaa tcttgagact gtctcagatg agattgatat gcggatagta aatatcatga
     4261 ctccaattgg caagggacag cgtggattga ttgttgctcc tccacgaacc ggtaaaactg
     4321 tgcttctgca aaagttagcg aacgcgattg ctactaatca tccggaggtt gttttgattg
     4381 ttcttctgat tgacgaacgt ccggaagagg taaccgatat gcaacgttcc gttaaaggag
     4441 aggttgtctc ctctaccttt gacgagccag ctcaacgtca tgtccaggtt gcagagatgg
     4501 ttatcgaaaa agccaaacgt cttgtagagc ataaacatga cgttgtaata ttgcttgatt
     4561 ccattacaag gcttgctcgt gcttacaatc aggttgttcc aacttcgggc aagatactct
     4621 ctggtggtgt agactcgaat gctttacacc gaccaaaacg tttttttggt gctgcaagaa
     4681 atattgaaca aggtggaagc ctaacaattc ttgcgacggc gctggttgaa acaggtagcc
     4741 gaatggacga agtaattttt gaagagttta agggaaccgg taattcggaa atgcacctgg
     4801 atcgaaaact ggctgaacga agaatatatc ctgctatgga tgtgaacaag gctggtacaa
     4861 gaagggaaga gttattactc gaagaagatg agttgcaaaa gctgtgggtt cttagaaaat
     4921 ttatctcgac aatgagtacg gctgaagtta tagaattact ggttgacaag atgaaggtca
     4981 caaaaaacaa tagagctttc tttaagacta tgaatgggta aaggagatat tatgaaatcg
     5041 gatatacatc ctgtttatga ggaaactaca ataaaatgcg cctgtgggtt tgaagttaaa
     5101 tcgcgctcta caaaaaaaga tgttcatgtt gagatatgtt ctgcatgtca tcctttttat
     5161 acaaaagagc agcgtctagt tacaactact ggccgtgttg agaagtttaa gcaaaaatac
     5221 aaacttaagt aatttttatt tgtttaatat tggcatctgt ttaacctttt tgccgagaat
     5281 atgacaaaaa ataatgataa taccggaaaa tgtgctatag aatgcctttt ccggtttttt
     5341 tttgtgtaaa aaatacactt taaacgagat atcgaactgt attattttca catatgtgta
     5401 ataatgtaac tataaaatat aaaaaaatca tataactact tgaaaatcca gctttttcaa
     5461 gcccgatcgt gcttgaatat ttgttaaatt tttctttttt tccataaaat tgaataaaaa
     5521 catgttaaaa ttagttaaat actgataatt aagctttcta ttgaaaatgg cacgaatatt
     5581 gctttaataa tagtggtcac ttaattaaaa agtatgataa aaaacggtaa ttttagtttt
     5641 cggggaggaa ggtatgagtg caattataga tagaagcatt tgtgtagctg aaaagaatga
     5701 tatagatttc aaaccgacgc ctgtatcaga tgagtttgca aaagcgtaca gaggattggt
     5761 aagcagtatt gcaaagaatt atgccagtta ccaatgtcct tttgatgatc ttgttcaaga
     5821 aggttttatg gggttaataa gagcctttga gaaattcgat ccatctcttg gatgtaagta
     5881 cacaacatat gcaacatatt ggataaagca ttatataacc cgttatatag cgaagcatgg
     5941 gcgaatcgta aggcttccga ttaagaagag cgaactttac cgaaagatat gtaaggttcg
     6001 tgattatctt tcgcacgaga tgggcgaaac accgacatta gatcagatag ctgaatatgc
     6061 tgaagtatcc atggaagatg tgaaaagaat tatggatatt tttcaaccgg aattatcatt
     6121 cgaaactcca tttcgtgatt caacatctga caggtacgat gttgttgaag atgaggttaa
     6181 tgatggcccg gaacatgcgt atttcaatga agaactaaag cgtgaattga agcatgctct
     6241 atcgcaactg atggataagg agagaatgat aataacattg cgcttcggat tagatacagg
     6301 tgtttctgta acgctgaaag atgttgcaga cagttttggt atctcttcag aaactgttcg
     6361 tcagatcgaa aaacgggctc ttgttaaaat aaagacacgc tttgcgtatt taaaggaata
     6421 tctttataac taaacaatag ttttaagcaa gctgaaatta tatctcgttt tgggatctgt
     6481 tttcagtttg cttcctttgt ccttttactg aattttgcgt gtgccatgat cgcatccagg
     6541 ttcggttgaa ttatgttacc cacttttttc ccccgtattg gtagagcccc atagttctca
     6601 aaaagattct ggtttcctga tgcctcttcg ggatattctg agtagtgagt cgcgtataga
     6661 ggagttgata ttttgctgat atgcttcact gttggggtaa tcacggggtc gtcttttttc
     6721 atttcgatta tataaattgc atctgaaagt tctgaaagca gttcgtttct ataaatagca
     6781 ttatgctttg atatctcttc ttttggaaaa aagggtgata caatagtgca ggtgtccgga
     6841 tcgaaaatct cttttaagtt atctgacatg ttgaaggtaa gcaggccaca aggaataatc
     6901 gcgaggagct cacctcgatt ttccagagtt ccagaggcaa atagtgttcc cgcgccacgg
     6961 ttaagacctg taccggcgca gataccatgt tctgccagta ttttaccggt atggtaagcg
     7021 atcgtatcgg cagtactgct tatttcggct ccactcataa ctgttattaa aggcaattga
     7081 agatttcttt tgtttccgaa acagtaaagt agtggaggtg tatttattga attgctgttt
     7141 ttaagctgct ctggatagtt ttcctcaaaa aagagtacag gctcaatgcc ggcagttaaa
     7201 atttgttgat aaacatcttc ggtattttcc aataactgtt ggcctttcaa tatgccttga
     7261 accgttcgtt tgctaaaact gaattcgtct tcgatttccg attcagttaa ctccataata
     7321 tcttgtatcg atagaccggt agataatagt gatttataaa tctctttcat ggaggcgata
     7381 ccgattccgg gggcttcgct tagtgcaatc cagtagttgt atttgttcat aaccttaccg
     7441 atgttttctg attatttcga aaaagagatc attatgacca gttaataatt ataaatgaag
     7501 ttggtatatg gtaatctcaa ataaataatt ttatgccaaa tatttaatct ttgctaatat
     7561 ttgtcaaaca gctcatcaga caagtgcaaa aaaaattaac ttgaaaaata agtaacctct
     7621 tataatcgta acccaattgt aacaaagcgg atgataatta ctctgaaaag catatttggg
     7681 tgatgcgtat gcaggttctc tgttaacgca ataaaatctg taaaaatcaa aacttagata
     7741 aacatgccga tattacggca tgttttggta tatcactggt aataataaga ttatttggct
     7801 ggttaggttt tctttattac ctgtgatttt ttaaccaata aaagggggaa gttaaatgat
     7861 agaagtaaat aacctctcca ggtattacgg tgatttttgt gcggtaaaca atatttcgtt
     7921 tacaattaac aagggtgaaa taaccggatt acttggaccg aacggagcgg gaaaaacttc
     7981 aacaatgaga atgattacag gctttcttaa ggccagcagt ggtgatattc ttgttggaga
     8041 tgttaatgtt aatgacgatc caacagccgt gaaaaaaatg ataggctatc ttccagaatc
     8101 tgcccctcta tacggggata tgatggttta tgactaccta gtttacgcgg caaaaattca
     8161 tgatattgtt gagccggaaa caagaataaa tgaaactgca ggtttatgcg gtttgattcc
     8221 ggtaatgcat aagaatatta atgaactttc aaaaggatac cgacagcgtg tcggccttgc
     8281 tcatgccatg attcataatc ccgaaatttt aattcttgat gagcctacta atggtcttga
     8341 ccctaatcaa attatcgaaa tcagaaatct gattaaagag attgggaaag agaagactgt
     8401 aatattgtcc actcacattc ttcctgaagt cgaggctact tgtgacagga ttataattat
     8461 caatcaggga aaaatagttg ctgataatac aactgatcag attcaatccg cgagtgaaaa
     8521 ttctcttgct gttaaggtat ctttaacagg agcatctgtt gctgatgtta aagataattt
     8581 taaaagtata aatggaattt tatcagtacg tgaggctttt gaaaaagacg gactggtttc
     8641 ggtaattctt gatactgtaa gctctcgtga ccttagacct gaggttttta
//
LOCUS       DAOTGZ010000111         8672 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_3064
            NODE_9747_length_8672_cov_5.746664, whole genome shotgun sequence.
ACCESSION   DAOTGZ010000111 DAOTGZ010000000
VERSION     DAOTGZ010000111.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868088
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 8672)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8672)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:22:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,785
            CDSs (total)                      :: 2,751
            Genes (coding)                    :: 2,732
            CDSs (with protein)               :: 2,732
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 12 of 19
            Pseudo Genes (internal stop)      :: 5 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8672
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9747_length_8672_cov_5.746664"
                     /isolate="AMR_MDS_3064"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            45..2066
                     /gene="recG"
                     /locus_tag="P5123_08265"
     CDS             45..2066
                     /gene="recG"
                     /locus_tag="P5123_08265"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: protein motif:HMM:NF008168.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase RecG"
                     /protein_id="HRX16292.1"
                     /translation="MLDDPIVVLPAVGAKRAALFSDFLGVNTVEDLLFIRPYKYVDLS
                     SSKLIDQCLDGEYITLKGTVTSSRITGSSKKFLEITVSDESGSIKVLFFGGFKYHGEQ
                     LSLNTEVVLSGKVSRKGGPVMVHPEYELNDSSVNTGRILPYYSIPGAMKDKGVSSKVM
                     RKIIKHACEDHLSAEYDDILPDYYSSLSDLTLGKALYLNHFPMELSDIVKVRERLAFS
                     ELFFLQILVLHQKKLSSKTFTCSGIDIHINNFISLLPFTLTKDQHEVLKRLSTDIAKG
                     KVLNNLLQGDVGSGKTAVAAGLCYVLAKKQKQSAFMAPTSLLARQHYETLTKMLPDIN
                     IALLTGATTPKEREQILFGLESGSIDIAVGTHALIQNSVSFKDLSLAVIDEQHRFGIQ
                     QRFALRKKSQSVHLLSMSATPIPRSLALTLYGDMEVSFLKEKPAGRIPVKTILIPESR
                     TEAMYRSVEKYINLGQQCFFVLPLIEANEDSELSSVEEYSEKLKKRFKNHKISMLHGK
                     MRGEEKEKVMNDFLEKSSSILVSTTVIEVGIDVPNATVIVIVHPERFGLAQLHQLRGR
                     VGRGNLPSFCVLCYNDSIPETTLNRINVLAESDDGFYIAEKDLEMRGAGEITGLLQSG
                     FNSDFVYADIINDRDILLIAREKAQIEIDNSSTIDFKHNMNNRYLRIIS"
     gene            complement(2056..3711)
                     /locus_tag="P5123_08270"
     CDS             complement(2056..3711)
                     /locus_tag="P5123_08270"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRX16293.1"
                     /translation="MDDLDKLLLNVDNEVDFKTLKEDEIRTHEKTVRKGDRNIGDEQF
                     DKNSSDSFESLFEKLKEKKEIQLFSENHLNGLKPHWNHIIDFKNILSDLTLPDPVIFA
                     LRKHKYNLLRSIENIERIYEDFITTQSYQNIEKIAKQDYPRFFPAMELDHQRMQFLIL
                     KEIFLRLREVNSHIRKEWKDLASNIKVMTLANESGAFVARCEDIIRNGINCCKDADLF
                     LEFLAAILYIPRKDFDVLEKDIINRIYFRDSYDYDYKSQFKLEPSVKPRNSNTQQESK
                     NNKNHDKKIALEESTGHSEKDNQGHNVNPEITEKIRNLYRVPGKSSWNTKEPYIIKID
                     LVKLDHNINDLESSIVFIPESKSHEAVDALVKRAMIRSMHAEINGITIRYEEFIFKTI
                     IKLVQNITSTYKEICEDMDLFIYHCAPMTVYNILLRRFHKESIGVIFKKISKLKIARI
                     LPSEYIKSLVMVWFNENVHTKELPYDRILDFNQIKTELQNRYNTEIENHLKKIKIAVE
                     HHREKTGVILDKNVILHKRAFDLFNDIEVEVYLRFLDKTIFRK"
     gene            complement(3727..5550)
                     /gene="ftsH"
                     /locus_tag="P5123_08275"
     CDS             complement(3727..5550)
                     /gene="ftsH"
                     /locus_tag="P5123_08275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008685173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent zinc metalloprotease FtsH"
                     /protein_id="HRX16294.1"
                     /translation="MNNTVKQLSLVAIIILLGYFIYTQVSTIKTDVAKVPYSQFIERL
                     NDNSVLSAKIFQNNTIKGKYISNGKELEYETIIPYQDPYIVQDLLKQGVELEGQAPDE
                     NQFLKSILGFLPWLLFIGFFWFIMMRQVQSTGNKAMSFGKSRAKLNAETTKKVTFEDV
                     AGVEEAKDELSEVVDFLKTPEKFSKIGARIPKGILLMGPPGTGKTLLARAIAGEAGVK
                     FFSISGSDFVEMFVGVGASRVRDLFEQGKKNAPCIIFVDEIDAVGRLRGAGLGGGHDE
                     REQTLNQLLVEMDGFESNEGVIIVAATNRPDVLDPALLRPGRFDRQVVVDVPDIRGRE
                     KILAVHSKNIPMEKDVDLKTIAKGTPGFTGADLANLVNEGALVAARNDKTRVGMHELE
                     EAKDKVLMGPERKSMILSDEEKRITAYHEAGHALIGHHLMKQDTIHKVTIIPRGRALG
                     LTMHLPEEEKHNYTRQYWLNMISFLFGGHVAEKLVFNETTTGSSNDIERATTIARRMI
                     CEWGMSDRFGPMAMGKRNDQPFIGRDMGHDRDYSEATAQAIDEEVLKILQEREKEATR
                     VIETEKVVFEKMAEYLIERETLNSQELKLIFDGESLPPLDE"
     gene            complement(5632..7017)
                     /gene="tilS"
                     /locus_tag="P5123_08280"
     CDS             complement(5632..7017)
                     /gene="tilS"
                     /locus_tag="P5123_08280"
                     /EC_number="6.3.4.19"
                     /inference="COORDINATES: protein motif:HMM:TIGR02432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016879 - ligase activity, forming
                     carbon-nitrogen bonds [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA lysidine(34) synthetase TilS"
                     /protein_id="HRX16295.1"
                     /translation="MGINNPVTCEKLKVFFDKYCEGTSGKSFLIAISGGKDSMVLTHL
                     MLGIREKVDCELHAFHLDHNTRNGESEKDAIFVQGYCDENRITYHGEKHVFTNSSNFE
                     EQARIVRYNCIEKLLDKYKIDFALTAHTLSDSIETVLMRIFRGTSVHGLQGIPKKRER
                     YLRPLIHYNSDEIIDYANKNHIVYRRDYSNESCDYDRNFIRNRVIPLVETRFPLESSI
                     KGLISNAVDISDLIIKSIKRDIEYRCENDSVVLKKFNELDDQSQFKHTFAFLIRDVLN
                     MKITSSLLSELYRNYIVNKTHLTIYKNKELEIKKTLLNNEPVIIFSKGFHNSSEIRPA
                     VVSTKIINRFSVCYSKKDYFVRLCDYDQKNEIEDGVVLAFNNQKELILRPRIPGDRIC
                     HNKMTVKLKKICINLKMSAEQKNQVIVVTLDNKVAAFIFDPYLDNYMIDDSFLTFDKT
                     KKILAINCRQK"
     gene            7198..>8672
                     /locus_tag="P5123_08285"
     CDS             7198..>8672
                     /locus_tag="P5123_08285"
                     /EC_number="5.4.2.-"
                     /inference="COORDINATES: protein motif:HMM:NF014877.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016868 - intramolecular transferase
                     activity, phosphotransferases [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phospho-sugar mutase"
                     /protein_id="HRX16296.1"
                     /translation="MKENISSKAIAWTKSPYDSETRKEIEILLSDNNQKELEDRFYRD
                     LSFGTGGLRGKIGAGSNRMNRYTVGLATQGLAVYLLKNNLANMGVVIAYDSRNYSKEF
                     AEETASVLAGNGIKSYLFDKLTPVPLCSFAMRSLSAAAGVVITASHNPPEYNGYKVYS
                     SDGCQIVPPQDSDIIGEISKIDNIESIKRADFSHSIDNKHINIIGDDIRKEYMQRVIE
                     SLSKPEEKKINIVYSPIHGSGITIIPELLSNAGFMNIHVVSEQEKPDGNFPTVRYPNP
                     EEKEAMSLAIRDGKAIDADIILATDPDADRIGVGVKQNEEYVLLSGNQLAALLCDYIL
                     TNLYEQNEIDDKTAVVKTIITTELLRAICDFYSVKTYDVLTGFKWIGALMRDFEQSHN
                     DNFVFGCEESYGYLAIDYVRDKDAVSSTLLVCEMAEKYRLKGLTLIDRLNQIYLKHGY
                     YCEQMRYIVLEGADGAGKIKTIMENFRNDPPYQVDGKDLETV"
BASE COUNT         2701 a         1648 c         1520 g         2803 t
ORIGIN      
        1 atacccaata gcgtctggta tatagcagag gaaacgacag aataatgctg gatgacccta
       61 tcgtagttct cccggcagtt ggggccaagc gtgcagctct attcagtgat tttcttggag
      121 taaatactgt agaggatctt ttgtttataa ggccctataa gtatgttgac ctctcttcca
      181 gcaagctgat tgatcaatgt ctcgacggag aatatattac tcttaaaggg acggttactt
      241 cttcacgtat aacaggaagt tcaaaaaaat ttctggaaat tacagtatct gacgaaagcg
      301 gaagcataaa ggttcttttt ttcggtggat ttaaatacca tggtgaacag ctttcactca
      361 ataccgaagt tgttttgtcg ggtaaagtca gtcgaaaagg cggtcctgta atggttcatc
      421 ccgaatatga gcttaacgat tcttcagtga acacagggcg catactaccc tattattcga
      481 ttcccggagc gatgaaagac aaaggtgttt catcaaaagt gatgagaaag ataatcaaac
      541 atgcttgcga agatcatctg tctgccgaat atgacgacat actcccagac tattacagct
      601 ctttatctga tcttactctg ggaaaagctc tctatttaaa tcattttcct atggaactct
      661 ctgatatagt taaagtaaga gagcgccttg ccttttcgga gctttttttc cttcagatac
      721 tcgtactaca tcaaaaaaaa ttatcttcta aaacatttac ctgttcgggt attgatatac
      781 atattaataa ttttatatct ttgttaccgt ttaccttaac aaaagaccaa catgaagtac
      841 ttaaacgact atcgacagat attgccaagg gtaaagtatt aaacaacctt ttacagggtg
      901 atgtcggatc aggaaaaact gcagtggcag caggcctttg ttatgttttg gcaaagaaac
      961 aaaaacagag tgcctttatg gctcctacat cgctacttgc cagacaacat tatgaaaccc
     1021 tgacaaagat gctacctgat attaatattg ccctgttaac aggagccact acgcccaagg
     1081 aaagagaaca gatcttattc gggcttgaat ctggatcaat agacattgct gttggcactc
     1141 acgcattaat tcaaaattcg gtttccttca aagacctatc tttagctgtt atagatgaac
     1201 agcatagatt cggcatacag caacgttttg ctcttagaaa aaaatcgcaa tcggttcatc
     1261 tgctttcaat gagtgcaaca ccgattcccc gctcattagc tctcactctc tatggggata
     1321 tggaagtttc atttttaaaa gagaaacctg ctggccgtat tcctgtaaaa acaattctta
     1381 tccctgaaag ccgtactgaa gccatgtatc gttctgttga aaaatatata aatttagggc
     1441 aacaatgttt ttttgtactt ccgcttattg aagctaatga agattccgag ctttcttctg
     1501 ttgaagaata ttcagaaaaa ttaaaaaaaa gattcaaaaa ccataaaata tctatgcttc
     1561 atggaaaaat gagaggtgaa gagaaagaaa aagtcatgaa tgatttttta gaaaaaagtt
     1621 catctattct tgtctcgaca actgtaattg aggtgggaat cgatgttccc aacgctactg
     1681 tgattgttat tgtacatcct gagaggtttg gtttggctca actgcatcag ctaagaggaa
     1741 gggttggccg tggaaattta ccttcctttt gtgtattatg ctataatgat tcaattcccg
     1801 aaaccacact aaaccgaatt aacgttcttg ctgaatctga tgacggattt tatatagccg
     1861 agaaagatct ggaaatgcgt ggagccggag agataacggg attactgcaa tccggtttta
     1921 acagcgattt tgtttacgcg gatattatta acgacaggga tattcttttg attgcgcggg
     1981 aaaaggctca aattgaaata gataattctt caacaattga tttcaaacat aatatgaaca
     2041 atcgctatct aagaatcatt tcctaaaaat agttttatcc agaaatctta aatagacttc
     2101 aacctctatg tcattgaaaa gatcaaatgc ccgtttatgt agtatcacat ttttatcgag
     2161 aattacacca gttttttctc tatggtgttc aacagctatt ttaatttttt tcaaatgatt
     2221 ttcaatttca gtattataac gattctgtaa ttcggttttt atttgattaa aatcaagtat
     2281 tcggtcgtag ggcaactctt tagtatgtac attttcatta aaccagacca ttaccagact
     2341 tttaatatat tcggaaggca gtatcctcgc aattttaagc tttgaaatct ttttaaaaat
     2401 aacaccaata gattctttgt gaaaccggcg aagaaggatg ttgtaaaccg tcattggcgc
     2461 gcaatgatat ataaacagat ccatgtcttc acatatttcc ttatacgtac tcgttatgtt
     2521 ttgaacaagt ttgataatgg tcttgaatat aaactcttca taacgaattg taattccatt
     2581 tatttcggca tgcatggatc ttatcattgc ccgcttgaca agagcgtcta ctgcttcatg
     2641 acttttagac tcgggaataa atactataga agattcaaga tcattgatat tatgatcaag
     2701 ttttacaaga tctattttta ttatataggg ttctttggtg ttccagctac tcttgccagg
     2761 aacacggtaa agatttctta ttttttcagt gatttcagga ttaacattgt gcccctggtt
     2821 atctttttct gaatgaccgg ttgactcctc taaagcaatt tttttatcat gatttttatt
     2881 attttttgat tcttgctgag tatttgaatt tcgcggtttt acgcttggtt caagtttaaa
     2941 ctgactcttg taatcataat catatgaatc acgaaaatat attctgttaa ttatatcctt
     3001 ttccagaacg tcaaaatctt ttcgaggaat atatagtatt gcagcaagga attctagaaa
     3061 aagatcggca tctttacagc agtttatgcc gtttcttata atatcttcac atctcgcaac
     3121 aaatgctcca gattcgtttg caagcgtcat taccttgata ttcgaagcca gatctttcca
     3181 ctctttacga atatgagaat ttacttctct taaacgcaaa aaaatttctt ttaatattag
     3241 aaattgcatt ctctgatgat ctaattccat agctgggaag aaacgggggt aatcctgttt
     3301 ggcaattttt tctatatttt gatacgattg tgttgtaata aagtcttcat atattctttc
     3361 gatattttca attgatctaa gaagattata cttgtgtttt cgtaaggcaa agattaccgg
     3421 atccggcaaa gttagatcag acaatatgtt tttgaaatcg attatgtgat tccagtgagg
     3481 tttaaggccg tttaaatggt tttctgaaaa taactgaatc tctttttttt cttttaattt
     3541 ttcaaaaaga gattcaaatg agtcagacga atttttatca aattgttcat caccgatatt
     3601 tcgatcacct ttacgaacag ttttttcatg agtccgtatc tcgtcctctt ttaatgtctt
     3661 aaaatcaact tcgttatcca catttaaaag aagtttgtca agatcatcca tcggcaatta
     3721 tcccgtttat tcgtctagag gaggtaaaga ttctccgtca aaaattagtt taagttcctg
     3781 tgaattgaga gtctctcttt caataagata ctctgccatt ttttcaaaaa ctaccttttc
     3841 agtttcaata acccttgtag cctctttttc gcgctcttga agaattttaa gaacctcttc
     3901 atcaatggct tgagcagtag cttcactgta atccctgtca tggcccatat cacgacctat
     3961 aaacggttga tcatttctct ttcccattgc cattggccca aaacgatcgc tcatacccca
     4021 ttcacatatc atgcgcctgg cgatggttgt cgcgcgctct atatcgttac tcgaacctgt
     4081 agtagtttcg ttaaaaacaa gtttttcggc aacatgtcct ccaaaaagga acgagatcat
     4141 gttcagccag tattgacgcg tatagttatg cttctcctct tcaggcaaat gcattgttag
     4201 ccccaaagct ctaccgcgag gaattatagt gactttatgt attgtatcct gcttcatcag
     4261 atgatgacca attaaggcat gtccagcctc atgatatgca gtaatgcgct tttcctcatc
     4321 agatagaatc attgatttac gctcaggccc catgagcacc ttgtctttcg cctcttcaag
     4381 ctcgtgcatg ccgacacggg tcttatcgtt acgcgctgct acaagcgccc cttcgttcac
     4441 aagatttgca agatctgctc ctgtaaaacc tggtgtacct ttcgcaatgg tttttagatc
     4501 cacatctttt tccataggaa tattttttga gtgtacggcc agaatttttt cacgtccacg
     4561 gatatccggt acatcaacaa ccacctgacg gtcaaatcgt ccgggcctta gcagtgctgg
     4621 atcaagtaca tcgggtctat ttgttgccgc tacaataatt acaccctcat tagattcaaa
     4681 accgtccatt tcaaccagta actggttaag agtctgctct ctttcatcat ggcctccgcc
     4741 aagaccagca ccacgtagcc tgcccacagc gtcaatttca tccacaaaaa taatacacgg
     4801 agcattcttt tttccctgtt caaaaagatc acgaacacgc gaagctccaa caccaacaaa
     4861 catctctaca aaatccgaac ctgaaataga gaaaaatttt acaccagctt caccggcaat
     4921 tgctcgtgcg agaagagttt tacctgttcc cggagggccc atcagcagaa tacctttagg
     4981 tattcgtgcg ccaattttgc tgaatttttc gggagttttc agaaaatcaa ctacttcaga
     5041 tagttcatct tttgcctctt ctacacccgc aacatcttca aaggtaactt ttttagttgt
     5101 ttctgcattc aacttggctc tgctctttcc aaaagacatt gccttgtttc cagtagactg
     5161 aacctgacgc atcatgataa accagaaaaa accgataaac aatagccatg gcaaaaatcc
     5221 aagaatggat tttagaaact gattttcgtc gggcgcctgt ccctcaagct caacaccttg
     5281 cttaagcagg tcttgcacaa tatatggatc ctgataggga attattgttt cgtactctag
     5341 ctcttttcca ttggaaatat atttaccctt aattgtatta ttctgaaata tctttgccga
     5401 tagtacagaa ttatcattga gcctttctat aaactgagaa taaggtacct tggcaacatc
     5461 agtttttatt gtagatacct gcgtatatat aaaatagcca agaagaataa tgattgcgac
     5521 cagagacagc tgtttgacag tattattcat taataactcc tgagtgatta taatgtcaat
     5581 atatcccttt ttttgaaaaa aacagagaaa agtgacgcta aagctttact tctatttttg
     5641 ccgacagttg atggcaagta tttttttagt tttatcaaaa gttaaaaatg agtcatctat
     5701 catataatta tcaagataag gatcaaatat gaaggcggca accttattat caagtgttac
     5761 gacaatcacc tggtttttct gttcagctga catcttgaga tttatacaaa tttttttaag
     5821 cttaactgtc attttattat gacatatcct gtctcccggg atacggggtc ttaaaattag
     5881 ttctttttga ttattaaaag ccagcacaac accatcttct atttcatttt tttggtcata
     5941 atcacacaaa cgtacaaaat aatctttttt tgagtaacaa actgaaaatc tgttaattat
     6001 ttttgtggat acgaccgcag gtcgaatttc tgaactatta tgaaaaccct tactaaaaat
     6061 gattaccggc tcattattca ataatgtttt ttttatctct aattctttat ttttataaat
     6121 agtaagatgt gtcttattga ctatataatt acggtatagt tcagacaaaa gagacgaagt
     6181 tattttcatg tttaaaacat ctctaatcaa aaaggcaaag gtatgtttaa attgagattg
     6241 atcatcgagc tcgttaaact ttttaagaac aactgaatca ttctcacaac gatattcaat
     6301 atctctcttt attgatttaa ttataagatc agaaatatca accgcgtttg aaataagtcc
     6361 tttaatagaa ctttcaagcg gaaatcgcgt ctcgacgaga ggaataactc tgtttcttat
     6421 gaaattgcgg tcgtaatcac atgattcatt tgagtaatca cgacgataaa caatatggtt
     6481 cttgttagca taatcaatta tctcgtcact gttatagtgg ataagcggtc taagatatct
     6541 ttcacgtttc ttcggtattc cctgtaaacc gtgaacagaa gttcccctaa atatacgcat
     6601 taaaacagtt tcaatagaat ctgataaagt atgcgcagta agcgcaaaat caattttata
     6661 tttatccagc aatttttcta tacaattata tcttacaatt cgggcttgct cttcaaaatt
     6721 cgagctgtta gtaaaaacat gtttttcacc atggtatgta attcggtttt catcacaata
     6781 cccctgtaca aatattgcgt ctttttcaga ttcaccatta cgcgtattat gatccagatg
     6841 aaaagcatga agttcacagt cgactttttc acgtatgccc aacatgagat gtgtcaaaac
     6901 catactgtct tttccaccgg aaattgcgat taaaaatgat tttcctgaag ttccttcaca
     6961 atatttatca aaaaagacct tcagtttttc acatgttacg ggattattaa tacccatgta
     7021 gctctccaag gtattgattc tcttttatct taacaaatta ttcttgcact aaggacaaat
     7081 gagtcaacta atgttttcta tttgacagac ttgatgaaaa caatgattaa ttacgggtct
     7141 gtaaattttt ttaactatat tattaaagta aaaagactag taattaaccg gtaaacaatg
     7201 aaagagaaca tatcaagcaa ggctattgcc tggactaaaa gcccctatga cagtgaaact
     7261 cgaaaagaga tagaaatatt attatcagat aataaccaaa aagagcttga agacaggttc
     7321 tatcgtgatc ttagcttcgg taccggaggt ttaagaggta aaatcggtgc cggaagcaac
     7381 aggatgaatc gttacacagt tggtctagca acccaggggc ttgcagttta ccttttaaaa
     7441 aacaatttgg ccaacatggg agtagttatt gcctatgatt cacgaaacta ttccaaagaa
     7501 tttgccgaag aaaccgcatc agtacttgcc ggaaacggca ttaagtctta cctatttgac
     7561 aaacttacac cagttccact ctgttcattt gccatgcgta gtctctcggc tgccgctgga
     7621 gttgtaatta ccgcaagcca taatccgccc gagtacaatg gatacaaagt gtacagcagt
     7681 gacggttgcc agattgttcc tccgcaagac agtgatatta ttggcgagat atcaaaaatt
     7741 gacaacatcg aatcaataaa acgagccgat ttttctcatt ctatagataa caaacatatt
     7801 aatattattg gggatgatat tcgtaaagaa tatatgcaga gagtaattga atcactctca
     7861 aaaccagaag agaaaaaaat caatattgtc tattcaccaa ttcatggaag cggaatcaca
     7921 attataccag aactgctttc aaatgccgga tttatgaata ttcacgttgt cagtgaacag
     7981 gaaaaaccag acgggaattt cccaacagtc agatacccca acccggaaga aaaagaggcc
     8041 atgagtcttg cgatcagaga cggaaaggct attgacgcgg atataatatt ggcaactgat
     8101 cctgatgcag acagaattgg tgttggagta aaacagaatg aagaatatgt gctgttaagt
     8161 ggaaatcagc ttgcagctct tttatgtgac tatattctta caaatttata tgaacaaaac
     8221 gaaatagatg ataagacagc tgtagtaaaa acgatcatta caactgaact acttagagca
     8281 atttgtgatt tttattcagt taaaacatat gatgttttaa ctggctttaa atggatcgga
     8341 gccctgatgc gtgattttga gcagagccat aatgataatt ttgttttcgg ttgcgaagaa
     8401 agctatggtt atcttgcaat agattatgtc agagataaag atgccgtttc ttcaactctt
     8461 cttgtatgcg aaatggcaga aaaatacaga ttaaaaggtt taacccttat tgatcgtctt
     8521 aatcagatat atttgaaaca tggatattac tgtgagcaga tgcgttatat agttttagaa
     8581 ggagcagatg gtgcaggcaa aataaaaaca attatggaaa atttcagaaa tgatcctcca
     8641 tatcaggtcg acggtaagga tcttgaaact gt
//
LOCUS       DAOTGZ010000112         8550 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_3064
            NODE_9926_length_8550_cov_4.308299, whole genome shotgun sequence.
ACCESSION   DAOTGZ010000112 DAOTGZ010000000
VERSION     DAOTGZ010000112.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868088
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 8550)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8550)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:22:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,785
            CDSs (total)                      :: 2,751
            Genes (coding)                    :: 2,732
            CDSs (with protein)               :: 2,732
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 12 of 19
            Pseudo Genes (internal stop)      :: 5 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8550
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9926_length_8550_cov_4.308299"
                     /isolate="AMR_MDS_3064"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..544
                     /locus_tag="P5123_08290"
     CDS             <1..544
                     /locus_tag="P5123_08290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019637003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HRX16297.1"
                     /translation="SGFLLGLPVSVIMVVVMLVLVNFLTRKTALGIFIESVGSNPTAS
                     FYLGINSRVIKLFSYLFCGICAGMAGLIFTADIKGADANNCGLWYELDAIASVIIGGT
                     VWGGRFTLLGTLLGAIIIQSITTTILSRGVAPEQILVFKAIVVMAVTLVQSSNMRKMV
                     VDSVFSVNKKTGKAVNKGLN"
     gene            544..1518
                     /gene="yjfF"
                     /locus_tag="P5123_08295"
     CDS             544..1518
                     /gene="yjfF"
                     /locus_tag="P5123_08295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018324774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease YjfF"
                     /protein_id="HRX16298.1"
                     /translation="MELKTVKNYIPLFATIFVFLIIYTMGVMLYDNFLSLRVFLNLFL
                     DNAFVGVIAVGMTFVILSGGIDLSVGSVVAFVCILSAKLLEVYQISPFLVVLVCLLFG
                     VFLGTVMGAVIHFFAVPDFLVTLVGMFLCRGLAYMVDLQSIPIRDKLFLAVNTMGINF
                     GGGVNFRLISIIFILVWAAGVYISHYTRFGRNIYAIGGDVQSANLMGIPIGKTRILVY
                     TMSSTLAALGGLLYAVYTLSGYAAAAQGLELDAIAAVVIGGTLLTGGVGFVEGTMVGV
                     LILGLIQTFISFQGTLSSWWTNIIIGLLIFSFILLQRFLSLRSDTDQK"
     gene            complement(1759..2457)
                     /locus_tag="P5123_08300"
     CDS             complement(1759..2457)
                     /locus_tag="P5123_08300"
                     /inference="COORDINATES: protein motif:HMM:NF020922.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2461 domain-containing protein"
                     /protein_id="HRX16299.1"
                     /translation="MAETSSYNGFSKNSIVFLNDILTNNKKEWLNDNRNRYEEFLLQP
                     MRQLVNDLAPTISIIDPQIDIEPKVGRTISKIFRDVRFSTDKSPLRANVWLTFKPRGS
                     DGRITPSFYFDLSYNNWSYGMGFYCAAPYVMRLFREKIMAKPDFFLNAVRTVSSNFEV
                     NGENYKRPPVINYPENLTVKQIQYLNPWLMKKSFYWSCTKKHDKDLFSSGLADIISKD
                     FITLSAIYSFLINQ"
     gene            complement(2450..2896)
                     /locus_tag="P5123_08305"
     CDS             complement(2450..2896)
                     /locus_tag="P5123_08305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011938463.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HRX16300.1"
                     /translation="MIKRKGGFLISKIHQVSGRIFNRILQEEGINGINSAQGRILFAL
                     WEQDGIPITTLSKKTMLERSTLTGMLDRLERGNFICRKPSPDDRRVLLIHRTDKDRDL
                     ERKYKQISESMCDLFYKGMSKDEINLFEANLENILDNCIEEETRNG"
     gene            complement(2899..3354)
                     /locus_tag="P5123_08310"
     CDS             complement(2899..3354)
                     /locus_tag="P5123_08310"
                     /inference="COORDINATES: protein motif:HMM:NF013412.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxamine 5'-phosphate oxidase family
                     protein"
                     /protein_id="HRX16301.1"
                     /translation="MTTKEKALEIIASAPSAVLTTINSDGYPESRALLNLRNALQYPS
                     LSKTINQLGDGSILIFTTNTGSSKMSQIINDNRVSVYYCLSESFQGVWVSGDIELISD
                     PLEKATFWQPGWEIYYPLGKFDPDYAILSLDPRRIRLYENLSVNEWRSS"
     gene            3767..4330
                     /locus_tag="P5123_08315"
     CDS             3767..4330
                     /locus_tag="P5123_08315"
                     /inference="COORDINATES: protein motif:HMM:NF017434.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent zinc protease"
                     /protein_id="HRX16302.1"
                     /translation="MISCFAEKIVVGEIEKIYLEEIDHLFTARIDTGAVTSSIHADNI
                     TRFERDGERWVRFDIEIIEKPKKKKSKKKSKDKDSEVVNKPKKIITVERPIVRKVKIV
                     QSNDDEAEKRIVVHFRYTIGTISQTAEFSLTDRKHLEFPVLVGRNILKDFMIVDVSKK
                     NICPPDLKKSSNKKIKVPVVRPSDKQE"
     gene            4415..5938
                     /locus_tag="P5123_08320"
     CDS             4415..5938
                     /locus_tag="P5123_08320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008486000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inactive transglutaminase family protein"
                     /protein_id="HRX16303.1"
                     /translation="MASKTSFYIIIALLLTLGFGLAIARYTYFGIPLTPDKFKKAWLL
                     EARVDFEAESGPVLAALTLPENPPGYKILGENSASPGYGFAIIEHDGKRRGEWSRNNV
                     NGPQTLYYKIQLVETGYDYNPQESDTVVADNVFWNETEDVAARYVVSTAGEKSSTPES
                     FTRQLIEIINNPEPEENAALLLSSYKPVELLYKLINYAGIPARISQGLLLEDARRRQQ
                     PVQLIEIFSDKRWISFDVVNKEQGLQPGYFLWQRGGDSLLDIVGGSNSRVTFSMIQSS
                     LSSLDIAATDIGQSGISWLGVHSLSVEEQGMFKFLFLLPLGVFVVVFMRLIAGIKTLG
                     SFMPALIALAFVQTSLFQGIVSFIVIVTVGLIIRSYLSYLNLLLVSRIATLIVIVIIL
                     VTFFSLAGYHLGLSTGMTITFFPMIIIAWTIERLSILWEEESGREVLKQGGGSLLVAV
                     VAYLVMTMPLAGHLTFNFPELSLVILAGIMLLGRYTGYRLSELYRFRDFKAASNEDN"
     gene            5925..6872
                     /locus_tag="P5123_08325"
     CDS             5925..6872
                     /locus_tag="P5123_08325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006787039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-L-glutamate ligase-like protein"
                     /protein_id="HRX16304.1"
                     /translation="MKIISPCSLKQFGILGINSRNRDYIARYNKRKYYPYVDDKLKTK
                     LLADVHGIPVPALKSIIRTQHDINLLDPVLSELNGFAVKPSRGSGGKGIWIIKEQKNG
                     NFIKSSGAVISKNDFKRHLSNIVAGLYSLNGSPDVVIVEDIIRISSVFSDYSYEGVPD
                     IRIIVFKGYPVMAMLRLATKVSDGKANLHQGAVGVGIDIATGSAVNAVWFSKTITRHP
                     DSNNDIRNIKIPGWKKILELASGCYEMSGLGYIGADIVIDKSKGAQLLELNARPGLSI
                     QVANGAGLIPRLKLIESVQMPFSSKEERVKFSIKNFSNY"
     gene            complement(6941..>8550)
                     /gene="metE"
                     /locus_tag="P5123_08330"
     CDS             complement(6941..>8550)
                     /gene="metE"
                     /locus_tag="P5123_08330"
                     /EC_number="2.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013134326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003871 -
                     5-methyltetrahydropteroyltriglutamate-homocysteine
                     S-methyltransferase activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0008652 - amino acid biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009086 - methionine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="5-methyltetrahydropteroyltriglutamate--
                     homocysteine S-methyltransferase"
                     /protein_id="HRX16305.1"
                     /translation="GKVYNCLCQAGTNNRIIVTTYFEHSCEATIELSKTDIWGLGLDF
                     VHGKDNLKSLTVIKDKTLVAGVVNGRNIWINDLSESLKLLDTISQKISKNKIIISTSC
                     SLLHVPYTKENEPHSDLTKWFAFAREKVKETALLAELFFCNDHKQYSDKITHNSNLIN
                     TRARSDMFTDQTVTQRIADISKTEREGNAAVRLQIQKKAFNLAELPTTTIGSFPQTAQ
                     IRKTRSDYKNERISQVEYDDQIKAYIDDCISFQENIGLDVLVHGEPERNDMVEYFGEM
                     LNGFHFTKKGWVQSYGSRCVKPPVIYADISRPAPMTVKWISYAQSKTNKMVKGMLTGP
                     VTIINWSFVREDIPRSIIAKQIAIALRDEIEDLQKAGIKIIQVDEAAFKEGYPLREES
                     VSDYEEWALESFKISVSSARKETQIHTHMCYSEFNDIIETIEKMDADVITIETARSGN
                     RLLRIFREREYKSEIGPGVYDIHSPRIPKTEEIRDEILKRLEVVDRDKVWINPDCGLK
                     TRRWEEVKPALLNMVEAAKALRLNNRT"
BASE COUNT         2352 a         1571 c         1790 g         2837 t
ORIGIN      
        1 aagcggtttt ttactggggt tgcctgtgtc agtaatcatg gtggtagtaa tgcttgtact
       61 ggtaaatttc ctaacacgta aaactgcatt gggaatattt attgagtctg tcggctcaaa
      121 ccctaccgcc agtttttatc tggggattaa tagcagggtt ataaaacttt tctcttatct
      181 tttttgcggt atatgcgccg gaatggccgg tctgattttc actgccgata tcaagggagc
      241 agatgccaac aactgcgggc tctggtacga gcttgacgca atagcttccg taataatcgg
      301 aggcactgta tggggcggaa gatttactct gcttggaact cttcttggag caataattat
      361 ccagagtatt accacaacaa tcctgtcaag aggggtcgcg cctgaacaga tacttgtttt
      421 taaggcaatt gtggttatgg ctgttactct tgtccagtca tcaaatatga gaaagatggt
      481 tgtcgattct gttttttcag ttaataaaaa aacaggaaaa gcagtaaaca aagggctgaa
      541 ctgatggaac ttaaaactgt aaaaaactat attcctttat tcgcgacaat atttgttttt
      601 ttaataatat atacaatggg ggtcatgctt tacgataatt tcctgtccct ccgtgtattt
      661 ttaaatctgt tccttgataa tgcgtttgtg ggtgttatcg cggtgggaat gacctttgtt
      721 attctgtcag gcggaataga tttatctgta gggtcggttg ttgcttttgt ctgtattttg
      781 tcggcaaaac tgcttgaggt ttatcagatt agtccgtttc tggtggttct tgtttgtctg
      841 ctgttcggag tatttctcgg aacagtaatg ggcgctgtta tacatttttt tgccgtacct
      901 gattttctcg ttactcttgt cggtatgttt ctctgccggg gattggccta tatggttgat
      961 cttcagtcga ttccaataag ggataaactg tttttggcag ttaatactat gggtattaat
     1021 ttcggtggcg gtgtaaactt cagacttatt tcgatcatct ttattctggt ttgggctgcc
     1081 ggtgtttata tatcacatta tacacgtttc ggcagaaaca tctatgctat cggcggcgat
     1141 gttcaatcgg caaatttaat gggaatacca ataggtaaaa ccagaattct tgtttataca
     1201 atgagcagta cccttgccgc gttgggcggt cttctgtacg cggtatatac tctttcaggt
     1261 tatgcggctg ccgctcaagg acttgagctt gatgccatag cggctgtcgt aatagggggg
     1321 actctgctga caggcggtgt cggttttgtt gagggcacca tggtcggagt tcttattctg
     1381 ggacttattc aaacatttat atcttttcag gggacattgt cttcctggtg gacgaatata
     1441 attataggtc tgcttatttt ttcatttatt ttactgcagc gttttttatc acttagatct
     1501 gatacggatc aaaagtaatt tacctcctga attactattc cgtaatcgcc ggtaaagccc
     1561 tgaatcccat acagtatgtg attcagggct ttctgattca gcttaactga cccgatttca
     1621 aaaattccgg ataatagaga acttcgaaaa attaccattt tccaaaaggc gacctcgttt
     1681 aagtgcctat tttaaaagga atttttcgca agttccttgt tgaaatgtta attaatagag
     1741 gtagtcaata catgcttatc actgatttat cagaaagctg taaattgcac taagtgtaat
     1801 gaaatcttta ctaataatat ccgcaagacc agagctaaac agatctttat catgcttttt
     1861 ggtacatgac cagtaaaaac tctttttcat taaccagggg tttaaatact gaatctgttt
     1921 cacggttaaa ttttcaggat agttaataac aggaggccgt ttgtagtttt caccgtttac
     1981 ctcaaaattg gaggatacgg tacggactgc attcagaaaa aaatctggct tagccataat
     2041 tttttctcta aacaacctca ttacatatgg agcagcgcaa tagaatccca taccgtaact
     2101 ccaattatta tatgacaggt caaaataaaa agatggtgta attcgcccgt cacttcctct
     2161 tggtttaaat gtcagccaca cattggcacg taaaggggat ttgtcggtag aaaaacgaac
     2221 atcacgaaaa atttttgaaa ttgtacgacc gaccttaggc tctatatcaa tttggggatc
     2281 aattatcgat attgttggtg ctaaatcatt aacaagctgc ctcatgggtt gcaaaagaaa
     2341 ctcttcatat cggttacgat tgtcattgag ccattctttt ttgttgttgg ttaaaatatc
     2401 gtttaaaaaa acgatactgt ttttcgagaa tccattataa cttgaagttt cagccatttc
     2461 ttgtctcctc ttcgatacag ttatcaagta tgttttcgag gttagcttcg aaaagattaa
     2521 tttcatcttt gctcatgcct ttgtaaaata aatcgcacat gctctcagat atttgtttat
     2581 attttcgttc aagatctctg tctttatctg tacgatgtat aagtaagact cttcggtcgt
     2641 caggagaagg tttgcgacaa ataaaattac cccgttccag tctgtctagc attccggtga
     2701 gcgtcgatct ttcgagcatt gtttttttgg acaaagttgt gataggaatg ccatcctgtt
     2761 cccaaagagc gaagagtatt cttccctgcg cagaatttat tccgtttatt ccctcttctt
     2821 gaagaatacg gttaaaaatt ctgccagaaa cctggtgtat ttttgatatc aagaatccgc
     2881 ctttcctttt aatcatagtt aacttgatct ccattcgtta acagagaggt tttcatacag
     2941 gcgtattctg cggggatcaa gactgagaat agcgtaatcc ggatcaaatt taccaagcgg
     3001 atagtatatc tcccagcctg gttgccaaaa agtagccttt tctaatggat cagagattaa
     3061 ttcaatatca ccggaaaccc agactccctg aaaagattct gaaaggcagt aatagactga
     3121 tactctattg tcattgatga tttgagacat ttttgatgaa cctgtgttag ttgtaaaaat
     3181 cagtatgctt ccatcaccaa gctggtttat cgttttagaa agagatgggt actgcaaggc
     3241 gtttctcaaa ttaagtagag ctcgtgattc aggatatcca tcactattta ttgtagttaa
     3301 aactgctgat ggtgcggagg cgataatttc taatgctttt tcttttgttg tcatgttgcc
     3361 cctatagctt aaaatattct atatcgtata ttactatata gtaatataat ttgtcaataa
     3421 taattcaata taattttaga tagttctata acaatactgt attcacttat ggttattaaa
     3481 atgtttcatc cgcctgggtg gatcagaata aattgttatg ctataattcg taatctaaat
     3541 tattgttgca attattaacc ttagactaaa agttattcaa tttggttgtt ggtgttaata
     3601 ttttaagttt gccatttata aatttatatg gtgtgtcatt tcataatagg gaactggcga
     3661 atggttattt ttcaaggtta ccaataatta ctatatgcgt tgaggtgctg ttattaacag
     3721 attattgata aatatcttta tagtagcggg tttggtctac tttaccatga tttcctgttt
     3781 tgccgaaaag attgtagtcg gcgagatcga aaagatatac cttgaggaga tcgatcatct
     3841 ttttacagca cgtatcgata ccggtgctgt gacctcgtcc attcatgccg acaatattac
     3901 ccgttttgaa agggacggtg agcgatgggt acggttcgat atcgaaataa tcgaaaagcc
     3961 caagaagaaa aaatctaaaa agaaatctaa agacaaagat tctgaagtcg tgaataaacc
     4021 gaaaaagatt attactgtag aaaggcctat tgtcagaaag gtaaaaattg ttcaatcaaa
     4081 cgatgatgag gccgaaaagc gaattgttgt gcatttccgt tacacgattg gtactattag
     4141 tcaaactgcg gaattttcgt taaccgatcg taagcatctt gagtttccgg ttctggttgg
     4201 ccgcaatatt cttaaggatt tcatgatagt cgatgtgagt aaaaaaaaca tctgcccgcc
     4261 tgatctgaaa aagagcagca acaaaaagat aaaggtgccg gttgtacgtc catcagacaa
     4321 gcaggagtaa gctctacaat tttaagcaac tttaagatat aatttcagag tcagtaatcg
     4381 taaagcgcgt cacactgtgg acttggaagg ttttatggca tcgaaaacaa gtttttatat
     4441 aatcatagct cttttactga ctttgggctt tggacttgct attgcacgat atacatattt
     4501 tggaatacca ctgacacctg acaaattcaa gaaggcctgg cttttagagg cgcgtgtaga
     4561 ttttgaagcc gagagtggac ctgttcttgc cgcgttgaca ctacctgaaa accctcctgg
     4621 ctataaaata cttggagaga attcggcttc tccaggttac ggttttgcca taattgaaca
     4681 tgatggtaaa agacgcggtg agtggtcacg taataacgta aacggtccgc agactcttta
     4741 ctataaaata cagcttgtcg aaactggtta cgattacaat ccgcaggaaa gtgatacggt
     4801 tgttgctgat aatgtttttt ggaacgaaac agaagatgtt gccgctcgct atgtggtatc
     4861 gacggcagga gaaaaatcaa gtactcccga aagttttacc cgtcaattaa tcgagataat
     4921 aaacaacccg gaacctgaag agaatgcggc tctgctttta tcttcttata agccggtaga
     4981 gcttctttac aagcttatta attacgctgg tattcctgct cgtatttcgc aagggctttt
     5041 actagaagat gcccgaagac gacagcagcc ggttcaattg atcgaaattt tttcagacaa
     5101 gcgatggatc tcttttgatg ttgtaaataa ggagcagggt ttgcagcctg gatatttttt
     5161 atggcagcgc ggaggagact cgctacttga tatagttggt ggaagtaatt cacgtgttac
     5221 attttctatg attcagtctt cactctcctc tcttgatata gcggcaacag atatcggcca
     5281 aagcggtata agctggctcg gtgttcattc gctctctgtt gaggagcaag gaatgttcaa
     5341 gtttcttttt ttacttcctc ttggggtttt tgtcgttgtg tttatgaggt tgatagctgg
     5401 cataaaaact ctaggcagtt ttatgccagc tctaatagcg cttgcttttg ttcaaacatc
     5461 ccttttccaa ggaatagtca gttttatagt gatcgttact gttggactga ttatacgttc
     5521 ttatctctct tatcttaatc ttttgctcgt atcacgaatt gctacactaa tagttatagt
     5581 tattattctc gtaacatttt tcagtctggc tggttatcat ttgggactca gtactgggat
     5641 gacaattaca ttctttccaa tgataataat tgcttggacc attgaaagac tttctattct
     5701 ttgggaagaa gagagcggcc gcgaagtttt aaaacagggg ggagggagcc ttcttgtggc
     5761 tgttgttgct tatcttgtta tgactatgcc tcttgcaggc cacctgactt ttaactttcc
     5821 agagcttagt cttgttattc ttgccggaat catgcttttg ggtcgttata ccggatatcg
     5881 tctaagcgag ctttatcgat tccgagattt taaggcagcc tcaaatgaag ataattagtc
     5941 cttgttcgct aaaacagttc ggtattttag ggataaactc aagaaaccgg gactacatcg
     6001 caagatacaa taaaagaaag tattatcctt atgttgatga caaattaaaa acaaaacttt
     6061 tagccgatgt gcatggcatt cctgtacctg ccttaaaatc aattattcgc actcagcatg
     6121 atataaactt gttagaccct gtattgtcag aattaaatgg ttttgcggtc aagccgtcgc
     6181 gaggatcggg tggaaagggt atctggataa ttaaagaaca gaaaaatggt aattttatta
     6241 aatcctcggg tgcagtaata tcaaaaaatg attttaaaag gcatttgtca aatattgtag
     6301 caggattgta ttcgttgaat ggttcacccg atgtagtgat agttgaggat ataataagga
     6361 ttagctctgt tttttcagat tattcgtatg aaggtgtgcc cgatataaga ataatagttt
     6421 tcaaaggata tcctgtaatg gcaatgctta ggcttgcaac caaggtctca gatggcaaag
     6481 ccaaccttca tcagggtgct gtaggtgtag gtatcgatat agccacaggc agcgctgtta
     6541 acgctgtctg gttttcgaaa acaattacaa gacacccaga ttctaataac gatataagga
     6601 atatcaaaat tcccggatgg aaaaaaattc tggaactggc gtcaggttgt tacgaaatga
     6661 gcggtctggg ttacattggc gcggatatag taattgataa atcaaaaggg gctcagcttt
     6721 tggaacttaa tgcccgcccc gggttgtcaa tacaggtggc aaacggcgcg ggtttgatcc
     6781 cgcgccttaa actgatcgaa tccgttcaga tgccttttag tagtaaggaa gaaagagtaa
     6841 agtttagtat caagaatttt tctaattact gatactttgg tacactacca aaagcgtttt
     6901 ctaaattgct ggtcaaaaaa cagaataaaa cctgttaaca tcatgttctg ttatttaacc
     6961 ttagtgcttt tgcggcttcg accatattga gcagtgccgg ttttacctct tcccatcttc
     7021 ttgtttttag accgcaatcg gggttgatcc agaccttatc acgatcaact acctctaacc
     7081 gcttaagaat ttcgtctctt atctcttcag tttttggaat tcggggagag tgtatgtcgt
     7141 atactccggg gccgatttcg cttttatact ctctttctct gaatattcgt aaaagtctat
     7201 taccgcttcg cgctgtttca attgtaataa catcggcgtc cattttttcg atagtttcaa
     7261 ttatatcatt aaattcacta tagcacatat gtgtgtgaat ctgagtctct tttctagccg
     7321 agctgacaga gattttaaaa ctttcaagag cccattcttc atagtctgaa acactctctt
     7381 cgcgaagcgg atacccctct ttgaaagcag cttcatcgac ctgaataatt ttgataccgg
     7441 ctttttgcag gtcttcaatc tcgtcgcgta gggcaatggc aatctgtttg gcaattatac
     7501 tgcgaggaat atcttcccgg acaaatgacc agttgattat tgtaacaggt ccggttagca
     7561 ttccttttac cattttgttg gttttactct gcgcgtagct gatccactta acggtcatcg
     7621 gggccggtcg tgagatatca gcgtaaataa ccggcggttt gacacaacgg cttccgtaac
     7681 tttgaaccca tccttttttt gtaaaatgaa atccgttcag catttctcca aaatattcga
     7741 ccatatcgtt tcgttcaggt tcgccatgaa ccaggacatc aagtccaatg ttctcctgaa
     7801 agctgatgca gtcatcaata taagccttga tctggtcatc atattctact tgagaaatac
     7861 gttcgttttt gtagtcgctt cttgttttac gaatttgcgc tgtttgcgga aaactgccta
     7921 ttgttgtggt tggcagttca gccaggttaa aggccttctt ttgtatttga agtcgaacag
     7981 ccgcgttacc ttcacgttcg gtttttgaaa tgtcagcaat tcgctgggta acggtttggt
     8041 cagtaaacat gtctgacctg gctcttgtgt ttataaggtt actgttatgg gtaattttat
     8101 cagagtactg cttatgatcg ttacaaaaaa agagttcggc caagagagcg gtttctttaa
     8161 ctttttcccg ggcaaacgcg aaccactttg ttaaatcgct atgtggttcg ttttcctttg
     8221 tgtacggaac atgcagaaga gagcagcttg ttgatataat gattttgttt ttggatattt
     8281 tctgtgaaat tgtatcaagc agtttgagtg attctgaaag atcattgatc cagatgtttc
     8341 gtccatttac cactccagcg acaagagtct tgtctttaat tacggtcaat gatttcaaat
     8401 tgtctttacc atgtacaaag tcaagaccca aaccccatat atcagtttta gaaagttcga
     8461 ttgttgcttc gcaggagtgt tcaaaatagg ttgttacaat gattctgtta ttagtaccag
     8521 cttggcataa gcagttataa accttgccga
//
LOCUS       DAOTGZ010000113         8391 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_3064
            NODE_10199_length_8391_cov_5.597049, whole genome shotgun sequence.
ACCESSION   DAOTGZ010000113 DAOTGZ010000000
VERSION     DAOTGZ010000113.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868088
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 8391)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8391)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:22:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,785
            CDSs (total)                      :: 2,751
            Genes (coding)                    :: 2,732
            CDSs (with protein)               :: 2,732
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 12 of 19
            Pseudo Genes (internal stop)      :: 5 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8391
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10199_length_8391_cov_5.597049"
                     /isolate="AMR_MDS_3064"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..474)
                     /locus_tag="P5123_08335"
     CDS             complement(<1..474)
                     /locus_tag="P5123_08335"
                     /inference="COORDINATES: protein motif:HMM:NF018004.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1015 domain-containing protein"
                     /protein_id="HRX16306.1"
                     /translation="MASIKAFRGLRPRADIASKLSELPYDVCNSEEAREISAGNEYNF
                     YHITKPEIDLPKDTDPYSSEVYSKAAENFKAFVEKGYLVEDEKELLYLYTLTMNGRTQ
                     TGLVTVVSVDDYDKGVIKKHELTRQAKEDDRIRHIDSVNAQTGPVFLLYKQKKEIR"
     gene            complement(489..977)
                     /gene="greA"
                     /locus_tag="P5123_08340"
     CDS             complement(489..977)
                     /gene="greA"
                     /locus_tag="P5123_08340"
                     /inference="COORDINATES: protein motif:HMM:TIGR01462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0070063 - RNA polymerase binding [Evidence
                     IEA];
                     GO_process: GO:0006354 - DNA-templated transcription
                     elongation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription elongation factor GreA"
                     /protein_id="HRX16307.1"
                     /translation="MSNDIEKRKQELRDELDALKHEFKVELPLRIAEAREHGDLKENA
                     DYHAARERQGFVQAKIAQLSDQLAKIASVNVSEIMDGVVGFGSEVTLADQDSSMTLTF
                     TLVTEAESNPSENKIAFSTPYGKALAGKKAGDIVEVTIPVGVKKFKIMKLKTIHGSEY
                     SA"
     gene            complement(1167..1304)
                     /locus_tag="P5123_08345"
     CDS             complement(1167..1304)
                     /locus_tag="P5123_08345"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRX16308.1"
                     /translation="MARIIDFIAFKLEKELKDEGFEVKMDHDKRIKLLLKFTGLKRKI
                     R"
     gene            complement(1787..2209)
                     /locus_tag="P5123_08350"
     CDS             complement(1787..2209)
                     /locus_tag="P5123_08350"
                     /inference="COORDINATES: protein motif:HMM:NF016160.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SoxR reducing system RseC family protein"
                     /protein_id="HRX16309.1"
                     /translation="MNECETGTVVEVQGDEYLIEPDLQEGCVSCRHRRSCHGSSGDQK
                     RCLICSSSIELSVGDRVAFNLVFPLIYASVLLYLIPAILIILGAILGSRYISISSDND
                     INTLMGILTALPVSAVLVFLLRRGKPVAHVVRRESDNI"
     gene            complement(2202..3059)
                     /locus_tag="P5123_08355"
     CDS             complement(2202..3059)
                     /locus_tag="P5123_08355"
                     /inference="COORDINATES: protein motif:HMM:NF016228.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar nucleotide-binding protein"
                     /protein_id="HRX16310.1"
                     /translation="MILLSGENRSIAHYLLPRLRDVHQVCSFGPDKGDINNGSFLNNI
                     FRDIAPTVFINLEQVDDSDDAEVYREWAYNSNSFAPLKIAEICSKQDIFLVHISTTEV
                     YPSDDKKLFDEDDTTDPVSVFSDSKLLGEKYIRESGCRYAILRLPCVYGINDNFLELF
                     FKTAFENRTIRIIKDQKFMPIYAGDVASAILSIIDKQIEGIYNFAGSDQVTMYQFLFR
                     AFDIYNEVKKTNLSPDVQEVPFEDYLSHADQLLANLVNSARFVGKTGINTVSYEEGLK
                     MFAKDLHYE"
     gene            complement(3068..3238)
                     /locus_tag="P5123_08360"
     CDS             complement(3068..3238)
                     /locus_tag="P5123_08360"
                     /inference="COORDINATES: protein motif:HMM:NF014470.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="twin-arginine translocase TatA/TatE family
                     subunit"
                     /protein_id="HRX16311.1"
                     /translation="MSMPGMGELVIIFFIILVLFGAGKIPRIAKDIGSGIKMFKKSLN
                     DDTDDSDNKADK"
     gene            complement(3315..5042)
                     /locus_tag="P5123_08365"
     CDS             complement(3315..5042)
                     /locus_tag="P5123_08365"
                     /inference="COORDINATES: protein motif:HMM:NF015894.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FapA family protein"
                     /protein_id="HRX16312.1"
                     /translation="MKLILLYFNDQAGKMLVQKQNAKGEKVVTVSSAVNFAARSEIIA
                     RVIDVNNESDTKKYLDQGYTYYKTVGFKKISMGPGVYFEEKDRSYRAQNYGFVTLNGS
                     GLLQVTAPIQVIKEKTAAVYYIVPTVHKRIPSVADINEQLTALKIVTNVDSKNIERDL
                     AKIDVNNPGVNRILVAKSIAPVHGRAEYYEPLIDLERKAGKVETDGHINFKEVNSIHE
                     VKKGDPVLKRFPKIDAQAGYTIFGEKAPAITEPTKGLLLGENLVPGKQNPDIFEASVD
                     GCIFLNNRKLSIKEIAIIDGDVDYESGNVNFHGSVQINGNVLPGFTVKAGGDIIIDGN
                     VDDAVIDAGGSVTITLGVAGKGRTEIIAGKDVNAKYLLNSKVEAEGTVAIDESVINCV
                     LVSNDKIIVTSKNGKIMGGKIVARNRIEANSVGSKKETVSDLMVGRNLKVEKIIDDLR
                     HELTEARESVSEQMNILKNSFGNNLFEDPKGFIAALPDIKKKNCVVMLNELTIRKSRE
                     SEVKEKIEETEKLLVFDEEPQILVYGNVYPGTVITVRREVKKIEETMTNARFYYDKED
                     KLIRFASCV"
     gene            complement(5454..6452)
                     /gene="fliG"
                     /locus_tag="P5123_08370"
     CDS             complement(5454..6452)
                     /gene="fliG"
                     /locus_tag="P5123_08370"
                     /inference="COORDINATES: protein motif:HMM:TIGR00207.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009433 - bacterial-type flagellum basal
                     body, C ring [Evidence IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA];
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar motor switch protein FliG"
                     /protein_id="HRX16313.1"
                     /translation="MNSIEKAAAFLISIGSEAASEILKNMDDDTVVAITKEMSRIEQL
                     SPEEKEEIIGDFMVDFNRIKNISYGGESFVRKTLVSSMGEEKAESIYNRIKTVYPEEG
                     FSFLKNVSSDILHNLVQNENSGTIALVFSNISSSQAGAVLQMLEPDKAKEVALRLARM
                     RKVSPEAAMQVASALKKRYDALTSADVLHDHPGGVDKLADILNHLDERTEQQIMSHFE
                     KEQPDTARKIAERIYSFESVLDLTNYEVRILIDNLDSDELLSIALKGAGDDIRVKFLR
                     NVSNNRATDIITEMERMGPVRMSDITKARREVVDIMRALDDAGTIVIKKNREKYVE"
     gene            complement(6445..7293)
                     /locus_tag="P5123_08375"
     CDS             complement(6445..7293)
                     /locus_tag="P5123_08375"
                     /inference="COORDINATES: protein motif:HMM:TIGR01944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RnfABCDGE type electron transport complex
                     subunit B"
                     /protein_id="HRX16314.1"
                     /translation="MSEQMIQTFAAITGITALGIIFGIILSIAKKKLHVEKDPRIEAV
                     NTVLPGANCGACGFPGCAAYAEKVVNEGVDIALCPVGGADCAEQIGKIMGLSASDMEK
                     KIARVHCQGGLFHTKNKYIYDGPRTCHAANQIRGGFRVCSYGCLGMGDCADVCPFNAI
                     TMGEDNLPSIDPDLCTGCGKCVEACPRGIISLLPVTQQVYVKCLNMEKGPVMKQGCSA
                     GCIGCRLCVVKACKNVFANNPDIESAITVDNFLAVVDGSLCINCGECSEICPQKVITF
                     PVKENE"
     gene            7479..8123
                     /locus_tag="P5123_08380"
     CDS             7479..8123
                     /locus_tag="P5123_08380"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRX16315.1"
                     /translation="MEISEKVKKLIDSTWEKNGEIVFVAYAMIEKTENSIKYLLEKTF
                     EGRETDYAVPVFSCIKELTTNSIKANIKKVLIDENIIDNPKNKEETLSKLKSILKEDI
                     LLEYSIKTKEYGLSTRVYLRKDDQQLVVRVINPVPLTYDQLERISRKMNASRNYDCLA
                     QFYLENPDPEAEGMGLGLSMVTVLLKGSGIDPDAFRVYSDMKKKTVAELSIPLV"
     gene            complement(8104..>8391)
                     /locus_tag="P5123_08385"
     CDS             complement(8104..>8391)
                     /locus_tag="P5123_08385"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRX16316.1"
                     /translation="SRLEQIAAFFDNVNVQLVKRPDYLVKTSQGRELLRRKIVSTVKR
                     RPSTIDDLMVIVGIARDELSAIIESYIDEGLIKAEEGERGTFYRAAKQEEY"
BASE COUNT         2582 a         1821 c         1369 g         2619 t
ORIGIN      
        1 gcgaatctct tttttttgct tataaagcaa aaaaaccggc cctgtttgcg cgttgaccga
       61 atcgatatga cgtatacggt catcttcctt tgcctgacgg gttaattcat gctttttaat
      121 gacaccctta tcgtaatcat cgaccgacac tactgtaaca agccccgtct gagtccggcc
      181 gttcattgtg agagtataca gataaagaag ttctttttcg tcttcaacca gatagccttt
      241 ttcaacaaag gctttaaaat tttctgctgc ctttgaatat acttcagagc tgtaaggatc
      301 tgtatccttt ggcaaatcaa tctccggttt ggttatatga taaaaattat attcatttcc
      361 ggcagaaatt tcacgcgctt cttcgctgtt acaaacatca tacggtaatt cacttaactt
      421 tgatgctata tctgcgcgcg gacgtaatcc tctgaaagct ttgattgatg ccataaaaaa
      481 ctatccccct atgcactgta ctcagaacca tgaatggttt taagtttcat tattttgaat
      541 tttttcacac ctacaggtat tgttacttcc acaatatcac cagccttttt tccggcaagt
      601 gcttttccat aaggagtact gaaagctatc ttgttctccg aagggttgct ttctgcctca
      661 gtaacaagcg taaacgtgag agtcatggac gaatcctgat ccgcaagagt tacctctgaa
      721 ccgaaaccta caactccgtc cattatttct gaaacattta cacttgcaat cttagcaagc
      781 tgatcagaga gttgtgctat tttcgcctgt acaaagccct gtctttctcg cgcggcgtga
      841 taatcggcat tctctttaag atcaccatgt tctcttgctt ccgcaatacg aagtggcaac
      901 tccactttga actcatgctt caatgcgtca agctcatcac gtaattcctg ctttcttttt
      961 tcgatatcgt tgctcatagc gcctctcatc ttattttaca agccatccac tactttttgg
     1021 acaaaaaaaa accggtaaaa ctacctaatt tatatcaaac aggtgatttt atatgatttt
     1081 cttgccggaa atatttgtgg aagagcgtta atatttttat tatagaacta aaaaaacaaa
     1141 tttatgtcaa gttatattta tctgccttac cgaatcttcc gttttaatcc ggtaaatttt
     1201 agaagaagtt ttattctttt gtcatgatcc attttaactt caaatccctc gtccttgagt
     1261 tctttttcaa gtttaaacgc gataaaatca ataatccgag ccacgataac taccttccct
     1321 agtttagtat tacaataaat ctattttaca gtaacgatca tcatctattt taaataatac
     1381 tacatatttc ttacatttgt agctgtttat tagctgtaaa acagatactt gtaacctcta
     1441 ttaatagaat tagaaacaag gaacttgcga ataattcctt taataaagct ggaaatagtg
     1501 atattttaat ctattttttt attatcggat attcatgggc cggtctttac gcgatgagga
     1561 acaaatcata tattgacaat ctctaataat taacttttct acaaggaact tgcccttgct
     1621 tattgttctt atcagaacaa taagctttta gcacaagata aaagccccac gggtaatacg
     1681 aaaaattcct ttacatatgg gctttaaacg gcatagccgt tctagaaatg gtaatttttc
     1741 gaagttccct attgaaagat aagctatgtt attgaaacta gaattatcaa atattatcag
     1801 actcgcgcct tacaacatgc gcgacaggtt tacctcttct aagaagaaaa accagtactg
     1861 ccgacacagg aagtgccgtc aaaattccca taagtgtatt aatgtcattg tcagaagaaa
     1921 ttgaaatata tcgactaccc aaaatagcac ctaaaataat taatattgcg ggtataaggt
     1981 aaagcaaaac tgaagcataa attagaggga aaaccagatt aaaagcaacc ctgtctccaa
     2041 cacttaattc tattgaagaa ctgcaaatta aacatctttt ctgatcacca gatgatccat
     2101 gacagcttct acgatgacgg cacgacacac acccttcctg caaatccggt tctataagat
     2161 attcatctcc ttgtacttca acaactgtac cggtctcgca ctcattcata atgtaaatcc
     2221 ttcgcgaaca tcttcagccc ctcttcgtaa gatacagtgt taattccagt tttacctaca
     2281 aaacgagcag aatttacaag attagctaaa agctgatcag catgcgacag ataatcttcg
     2341 aatggaactt cctggacatc aggtgagaga ttggtttttt ttacttcatt atagatgtca
     2401 aacgcacgaa acagaaattg atacatcgtc acttgatcgg atcctgcaaa attataaatg
     2461 ccttcgatct gcttatcaat tatactcaaa attgctgacg ccacatcacc agcataaata
     2521 ggcatgaatt tctgatcttt aataattctt attgttctgt tttcaaaagc agtcttaaaa
     2581 aaaagttcta aaaaattatc atttataccg tagacacatg gtaatcgaag aatcgcatat
     2641 cggcaaccgg attcgcgaat atatttttca ccaagaagct tactatctga aaaaacagaa
     2701 acgggatccg ttgtatcgtc ttcatcgaat aattttttat catcagacgg atacacctcc
     2761 gttgtagaga tatgaacaag aaagatatcc tgttttgagc agatctccgc tattttcaga
     2821 ggggcgaaac tattgctatt gtaagcccac tcacgataaa cctccgcgtc atcactgtca
     2881 tctacctgtt caagattgat aaaaacagta ggcgctatat ctctaaaaat attatttaaa
     2941 aaagaaccat tatttatatc gcccttatca ggaccaaaag aacaaacctg gtgaacatca
     3001 cgcaaccttg gcaataaata gtgtgctata gatctgtttt caccggaaag caatatcatc
     3061 tataaaccta tttatccgct ttgttatcag aatcatcggt atcatcattt aaactttttt
     3121 taaacatttt aatacccgaa ccaatatcct tggcaatccg aggaatttta cctgctccaa
     3181 aaagaacaag aattatgaaa aatatgataa ctagttcgcc cattcccggc atactcatac
     3241 taacctccag gttattttaa tactgaaaat aattctactt gtggtcaaga ttttttgaat
     3301 ggaaaaatgc tcagttaaac acatgacgcg aaacgtatca gcttgtcctc tttgtcataa
     3361 tagaaccttg cgttagtcat ggtctcttct atttttttaa cctcacgtcg cactgtaatc
     3421 acagtacctg gatacacatt tccgtaaaca agaatttgag gttcttcgtc aaaaacaagc
     3481 agtttttcgg tctcttcaat tttttctttt acttcgctct ctctactttt gcgtattgtc
     3541 agctcgttta gcatcacaac acaatttttc ttttttatat cgggaagtgc cgcgataaac
     3601 cccttcggat cttcaaataa attattgcca aaactattct tcaaaatatt catctgctca
     3661 gagaccgact cccgtgcttc tgtcaattca tgacgcaaat catcgataat cttttcaact
     3721 ttcagattac gcccaaccat aagatccgaa acagtctcct tttttgaccc taccgaattt
     3781 gcctctattc tgttgcgggc aacaattttt ccccccatta ttttaccgtt ttttgaagtt
     3841 acaataattt tgtcatttga aacaagaaca caatttatta ccgactcatc tattgcgact
     3901 gtgccctcgg cttctacttt ggagttaagc agatactttg catttacatc tttacccgcg
     3961 ataatttcgg ttcttccttt accggctact ccaagggtaa tagtaacaga gcccccagca
     4021 tcaatcacgg catcatcaac atttccatca ataataatat ctcccccagc tttaactgta
     4081 aaaccgggca aaacatttcc gttaatctgt acactgccgt gaaaattgac gttacctgat
     4141 tcataatcca catcaccatc aattatagct atctctttta tggaaagttt gcgattgtta
     4201 agaaaaatgc atccgtccac tgaagcctca aaaatatcag gattctgctt tcccggcacc
     4261 agattttcac ccaatagcaa tcctttcgtt ggttcggtta ttgctggagc cttttctcca
     4321 aatattgtat agccggcctg agcgtcaatc tttggaaatc gttttaagac aggatcccct
     4381 ttttttactt catggatgga attgacttct ttaaaattaa tgtgtccatc cgtttcaact
     4441 ttaccggctt ttctttcaag gtcaatcaga ggttcataat attcagcgcg accatgtact
     4501 ggagctattg atttagccac cagaattcga ttgaccccag gattatttac atcaattttg
     4561 gcaagatcac gttcaatatt ttttgaatca acattggtta ctattttcaa ggcggtcagt
     4621 tgttcattga tatcggccac agatggtatt cttttatgta ctgtaggaac tatataataa
     4681 acagcagctg tcttctcttt aataacttgg atcggtgctg taacctgaag caaccctgat
     4741 ccgttaagtg taacaaatcc gtagttttgg gctcggtaac tgcgatcctt ctcttcaaaa
     4801 tagactcccg gacccataga aatcttttta aaacctactg ttttataata agtatatccc
     4861 tgatccagat atttttttgt atccgattca ttattaacat ctattactcg ggcaattatt
     4921 tctgaacgcg cagcgaaatt tacggcagaa gatacagtga caactttttc accttttgcg
     4981 ttctgctttt gaacaagcat tttaccggcc tgatcattaa aatagagtaa aataagtttc
     5041 atctctcttt cctgtaatca tttgaatgac ttgtttaaac aacgctaact taataaaccg
     5101 aaaaaaggaa cagttataag ttatcaaatt gcgatcagtt taaaccctgt ctttaattat
     5161 cgttaataac tatccaatac ttaacctgtt aaccaaacga aggtcaacaa gcctaatttc
     5221 gcttaacgta aataaagacc ggttttcgaa gttcccggtt aatcaaaaaa tcatcaaaac
     5281 agtgttaata tactaaaaaa acagaatctt atcaatgatt caaaaataaa atttttaaaa
     5341 ttaaactatg attagcataa tcatcttgtt agtaaggaaa tattgcgaag tttttgtttt
     5401 ggtattaagt aattaaagaa gataatgaaa ccatataatt atgaaaaaga agactactca
     5461 acatacttct cgcgattttt tttgataact attgttcctg cgtcatctag tgccctcatg
     5521 atatcgacaa cctcgcgcct tgccttcgta atatcggaca ttcttacagg gcccattctt
     5581 tccatctcgg taatgatatc cgtagctctg ttattagaga cattccgtaa aaacttgaca
     5641 cggatgtcgt caccggcacc ttttaatgct atagacagaa gttcatcgct atctagatta
     5701 tctattaaaa ttcgtacttc gtagttagta aggtcaagaa cgctttcaaa cgagtatatt
     5761 ctttcggcaa tctttctggc agtatccggt tgctctttct caaaatgaga cataatctgc
     5821 tgttcggttc tctcatcaag gtgatttaga atatcagcaa gcttatctac tccaccggga
     5881 tgatcgtgaa gaacatcggc cgatgttaaa gcgtcatatc tttttttcaa ggccgaagca
     5941 acctgcatag cagcttcagg tgaaacctta cgcatgcgcg caagccttag agcaacctcc
     6001 tttgccttat caggttcaag catttgaaga acagcccccg cctgagaaga gctgatattt
     6061 gaaaaaacaa gcgctattgt tccagagttt tcattttgaa caaggttatg caaaatatcc
     6121 gaagagacat tctttaaaaa actgaaaccc tcctcgggat atactgtttt gattctatta
     6181 tagatggatt ccgctttctc ttcacccatg gatgagacca gagttttgcg cacaaacgac
     6241 tcaccaccgt aactaatgtt tttgatacgg ttaaaatcta ccatgaaatc accgataatt
     6301 tcttccttct cttcaggaga gagctgttca atacgggaca tttctttagt tattgcaaca
     6361 acagtatcgt catccatgtt ttttaatatt tccgaggcag cctcgcttcc tatagaaatc
     6421 agaaaagctg cagctttttc gatactattc attttctttt accggaaaag taatcacttt
     6481 ttgtggacaa atttcagaac actcgccaca gtttatacaa agagacccat caacgacggc
     6541 taaaaaatta tcaacagtta tcgcgctttc aatatcagga ttattggcaa aaacattttt
     6601 acaagctttt actacacaaa gcctgcaccc gatacacccc gcagaacatc cctgtttcat
     6661 aacaggccct ttttccatat ttaggcattt tacatacacc tgttgagtaa ccggtaacag
     6721 agaaataata cctcttgggc aagcctccac gcatttaccg caacccgtac aaagatcagg
     6781 atcaatagac ggaaggttat cctcccccat ggttatggcg ttaaaagggc aaacatccgc
     6841 gcaatcgccc attccaagac aaccgtacga acagactctg aaaccacctc taatttggtt
     6901 agcggcatgg catgtacgcg gtccatcgta aatatatttg ttttttgtat gaaacagtcc
     6961 gccctgacaa tgaacccgag caattttttt ttccatatca gaagcagaaa gccccataat
     7021 tttaccaatc tgttcagcac agtcggcacc accgactggg cacaaggcta tatcaacacc
     7081 ctcattgact actttttcgg catacgcggc acatcccgga aagccacagg caccgcaatt
     7141 tgcacctggc aatactgtat tgactgcttc tatacgagga tctttttcga catgaagttt
     7201 tttctttgca attgataaaa ttattccgaa tataatacca agggccgtta ttcccgttat
     7261 tgcagcaaaa gtttggatca tctgttcact cataaacggc tccataaaaa aaattttgac
     7321 ttgccaaccg cacagtttta caagaaattg ttgttaaata catttttggc gatataacag
     7381 ttattagtga attaaatttt gccggattat gtcaatattt attttatgca aacgatttca
     7441 taaaaagcac ggcataaccg tgattaacag gttattaaat ggaaattagt gaaaaagtca
     7501 agaaactgat agattctaca tgggaaaaga acggcgagat cgtttttgtt gcctatgcaa
     7561 tgatagaaaa aacagaaaac agtataaagt acctattgga aaaaacattt gaggggcgtg
     7621 aaacagatta cgcggttcct gtcttctcgt gtattaaaga gttaaccaca aactcgataa
     7681 aggcaaacat taaaaaagtt cttatcgatg aaaatatcat agataaccct aaaaacaaag
     7741 aagagactct ttccaaacta aaatcaatct taaaagaaga tattttactc gagtatagca
     7801 tcaaaaccaa agagtacggg ctctcgaccc gtgtttacct caggaaagac gatcagcaac
     7861 ttgttgtcag ggtaataaat ccggttcccc taacctatga ccagctagaa cgtataagcc
     7921 ggaaaatgaa cgcttcgaga aactacgatt gcttggcaca attttatcta gaaaaccctg
     7981 atcccgaagc agagggtatg gggctggggc tatccatggt tactgtactg cttaaaggtt
     8041 ctggtatcga tcccgatgct tttcgcgtat actctgacat gaagaagaaa acggtagccg
     8101 agctcagtat tcctcttgtt tagcagcgcg gtaaaatgta ccgcgttcac cctcttccgc
     8161 cttaatgagc ccctcgtcaa tataactttc aattatagct gacagctcgt cacgcgctat
     8221 gccaacgatt accatcagat catcaattgt tgacggcctt cttttaaccg ttgagactat
     8281 tttccgacgt aacagttcgc gcccctgtga agttttaaca agataatcgg ggcgcttaac
     8341 aagctgtaca ttcacattgt cgaaaaaagc cgctatctgt tcaagacggg a
//
LOCUS       DAOTGZ010000114         8363 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_3064
            NODE_10246_length_8363_cov_5.316803, whole genome shotgun sequence.
ACCESSION   DAOTGZ010000114 DAOTGZ010000000
VERSION     DAOTGZ010000114.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868088
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 8363)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8363)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:22:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,785
            CDSs (total)                      :: 2,751
            Genes (coding)                    :: 2,732
            CDSs (with protein)               :: 2,732
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 12 of 19
            Pseudo Genes (internal stop)      :: 5 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8363
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10246_length_8363_cov_5.316803"
                     /isolate="AMR_MDS_3064"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            106..777
                     /locus_tag="P5123_08390"
     CDS             106..777
                     /locus_tag="P5123_08390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018029026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HRX16317.1"
                     /translation="MSNVIEISELEKIYGIGRNALHVLNGIDLEIRHGEIVSVVGSSG
                     AGKSTLLHCIGCLDTFQKGSLKINGKELSGFNVEELSGFRNRTMGFIFQSNNLLTEFT
                     ALENVMIPLLIRRVSRKKAKLAALEVIDRFGLGERINHKPSEMSGGECQRIAVARAIV
                     GKPTLILADEPTGSLDSKNSEHLIDILFELGRENGTTIVIVSHDPLIAEKTGRTIHIK
                     DGKIC"
     gene            811..2430
                     /locus_tag="P5123_08395"
     CDS             811..2430
                     /locus_tag="P5123_08395"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006314208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HRX16318.1"
                     /translation="MKIEFVGAARTVTGSSYIVKNDDFTVMIDCGMFQGNRELRERNQ
                     LDLIYAPQEIDALLVTHAHIDHSGLIPKLVKEGFTGKIFATKATYDLLRIMLPDSAHI
                     QEMDAEFVNRRNRKLGRPPVEPLYTEPDALKSLEYIHPVKYGDDFEVTKGVRAVFRDA
                     GHILGSSFIELSVTENGKTKKIVFSGDLGPKDQAIIRNAENFTGADYLLIESTYGNRL
                     HKSKPDTYEEFKQIIHDAYNRKGNIVIPSFAVERTQEIIYTLAQLFRSGDIPKIPTYI
                     DSPLAISATEIFRENSECFDDDTRKILESGGSPLDFPNLHLTRTTAESKDLTKTARGS
                     IIISASGMCTAGRIKYHLQSNLYKPDSSIVFVGYQAEGTLGRKLVDGAKVVRVYGEDV
                     AVNAKIHTLGGFSGHADRDRLLEWMGTGDNPDMKVFVVHGEHESSENLASLIREKYSF
                     TTIVPTWGEIVDLDTFELSKAKYGEPLVEHTDLKEHGDAAIKEIETLMQEYISKYKKV
                     SKESPMVKSEEIKSNLDDLKQMITMLKDEIS"
     gene            2442..>3179
                     /locus_tag="P5123_08400"
     CDS             2442..>3179
                     /locus_tag="P5123_08400"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRX16319.1"
                     /translation="MPALIETITDPAHFQKIFYDFFLKGVVYLKTANGNFKIQFLGYS
                     EGLIALRIPIVKNMPASCVILCRIPGETIYAQLKFVEKQEDEAYILKPVKIQMISVSR
                     NEQRHSISGKGKQLLFVSKVISDSIIERSISFDQRKAELAKLKISDQLKSVFVSKKIY
                     FSNEGSSDDRMRYFYNRKPDYLIPDFDSIDIKDPVQMNFKNDILNKDYYIQNRRAVMS
                     EASVAVYYREAIPFGYIQVNHNKKLDKS"
     assembly_gap    3180..3189
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <3190..3534
                     /locus_tag="P5123_08405"
     CDS             <3190..3534
                     /locus_tag="P5123_08405"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRX16320.1"
                     /translation="DKSVLIMLKNLSMQLSKIFANVKLFKPLDENLLVSDISSKGVGI
                     VFRERSYIRFFPEKSYVHFELILPDQIRIPMLTVVRSITFLENKIIKAGCEIISMDKS
                     AMQIYSHYLQSL"
     gene            3549..5381
                     /locus_tag="P5123_08410"
     CDS             3549..5381
                     /locus_tag="P5123_08410"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HRX16321.1"
                     /translation="MRPLKLYPLSDYTLKIALLEDDPIILQDIKSMLVSLGHSVVATY
                     RSGEEFLAEYQFIRCDLFILDIELYGKKSGVDVAEEIQKSRSVPLIYLTSYSDDETVT
                     NILKTDPYGFILKPARAEHLRISINIAYYKFLSDKRIRESEQRYQSIVKTLPLMLARF
                     NPVNDTVTFCNDNFKDHFFDPFDKVHAGFFDKMSDLGFEDLIDIFSSLNRVSNVTIKE
                     YAIASNGVTGHFKVICQALYDSDGGLFEYQFLCEDISEVRERERKIRDYSSELDKKIR
                     ELKCLYSISNILQNNRNISKMLQRVVDEISLAMSNPYNVSSCVEYGVDSFYSYNFFSS
                     SANFTHPIIVRGSIVGQIHLHVEKNQERDDLFQKGELDLLITVSEMISKMLEKTEAEI
                     QLKKLEKEIIMTSEREKQNLGHELHDGIGQILTGASFILKSLEKKLQKEANVPQELFD
                     VVDLIKDATSRCRRLSKGLVPVSYSNETFVYLVEQLLHSTKEFYNITFELSMPENFDL
                     KNSFVKSQLFRIVQEAVNNTVKHAQASKITLILEDEKEWVRLAVSDNGVARAVKGKNP
                     GLGMNIMKYRADLIGANFDIIMEENVGTTVVVRLPGDMVVYGQN"
     gene            5411..6052
                     /locus_tag="P5123_08415"
     CDS             5411..6052
                     /locus_tag="P5123_08415"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HRX16322.1"
                     /translation="MKIMIVDDHPVVRKGIKSILESEDDINVTAEAESYDEALEKLGV
                     DLPDLAIVDIELKGSENGVALIKSIKDKYPQVKTLVMSMDDGSLYAERSIRAGALGYI
                     AKEEASERIVSAIHQVMSGELYLSSKLSKMIASSHIMGANDKGPDVTTLSNKEFEIFK
                     LIGQGYKRSEISKKMSMNINTIESHRRKIREKLDIKNSADLSRMAVKWYVDSQ"
     gene            6210..7016
                     /locus_tag="P5123_08420"
     CDS             6210..7016
                     /locus_tag="P5123_08420"
                     /inference="COORDINATES: protein motif:HMM:NF012876.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing protein"
                     /protein_id="HRX16323.1"
                     /translation="MTFSSKNRFSSFLSRFKRDKQTSRLKKKLLLYFILVAIVSVSVS
                     AQIILEMSSTRFRNQIRDGYISEISRELGSQVAVKLESVSTDAIAESVSQLRNRMLLM
                     LIVVSASIFGAFSLFVRDIVVPMDGLVEATKKIASGDLNAHAPVMSEDEIGQLAELIN
                     DMNINMQDLITQVRRELDNYNFEIAFAVAMIGQILSSENTGDALKHKRLKLSEFKRII
                     ELNKSVENVLDKMSDKLTSLHSFISTYTNYTISSEITQKEIDESLSHYRS"
     gene            7029..7829
                     /locus_tag="P5123_08425"
     CDS             7029..7829
                     /locus_tag="P5123_08425"
                     /inference="COORDINATES: protein motif:HMM:NF014035.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite exporter TauE/SafE family protein"
                     /protein_id="HRX16324.1"
                     /translation="MVIDFVLFELNMQYWYMFPVSILVAIICNSSGFSGGVLFQPIYN
                     IFLNIPIQNAVATGIATETVGMTSGALRYLWYKMIELPVGFTMIMLTIPGVVLGNFAL
                     LVIDEDLLKLILGIIIVGLATTQLFGAIRKHFGTKENIPIEDMYSYLWVPPIGGFFSA
                     ATGTGICELAQPVLEKGLKIKTKKANATAILVEATGDWIITILNLHAGLIMWELWIFT
                     GTGVILGGQIGPYISKYLPDRVLKFVFSICVIAVGIFYIIKGVNWLSK"
     gene            7972..>8363
                     /locus_tag="P5123_08430"
     CDS             7972..>8363
                     /locus_tag="P5123_08430"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRX16325.1"
                     /translation="MENIKENIGSCQQLSISITSTATDVNKKSIERKDLTEAGRDIIE
                     QLIERNDTMIDNFETVLSNLQDSQRAISNNSENIVNNKEILVSIVTRLKTVGGELSQM
                     ENEIEKLTEIVDEIKGDTNKIFSLALNAS"
BASE COUNT         2629 a         1362 c         1768 g         2594 t
ORIGIN      
        1 gttttgtaat ctttattgcc gtatttgccg tgtttctttc cactgttgct tcaatttttc
       61 ccgcctggca tgcgtcgcgt ttaaaacctg tggagacaat cagatatgag taatgttata
      121 gagataagtg agcttgaaaa aatatatgga attgggcgca atgctcttca tgtattaaat
      181 ggtattgatc tggaaataag gcatggagaa atcgtatctg ttgtcggttc ctcgggggcc
      241 ggcaaatcga ctttgctgca ctgtatcgga tgccttgata cttttcaaaa ggggtctctt
      301 aaaattaacg gtaaggagtt atctggattc aatgttgaag agttgtcagg ttttcgtaac
      361 cgtacaatgg ggtttatttt tcagtcaaat aatcttttaa ccgaattcac agcacttgaa
      421 aatgtcatga ttcctctgtt aattagaagg gtaagccgga aaaaggcaaa gttggctgct
      481 ctggaagtga tcgacaggtt tgggcttggg gagcggataa atcacaaacc ttcagaaatg
      541 tccggtggag aatgtcagcg catagctgtt gcgcgtgcta ttgtcggaaa accgacactt
      601 attttagctg atgaaccaac aggaagtctg gatagtaaaa attcagaaca tttgattgat
      661 atactatttg aattgggcag ggaaaacgga acaacaattg ttattgtgtc acatgatccg
      721 cttattgctg aaaagacagg caggacaata catattaaag acggaaaaat atgctaatca
      781 atttctaata cttagcgaaa tggaggctct atgaagattg aatttgtcgg agcggcgcgt
      841 acggttacag gttcgtcgta cattgtaaag aatgatgatt ttactgttat gattgattgt
      901 ggtatgtttc aggggaacag ggaacttaga gagcgtaatc agctcgattt gatctatgct
      961 ccacaggaaa ttgacgcact acttgtaact catgcccata tcgatcacag cggattgatc
     1021 ccaaagttgg taaaagaagg ttttaccggc aaaatattcg cgacaaaggc aacctatgat
     1081 ctattaagaa taatgcttcc tgattccgcg catattcagg agatggacgc ggagtttgtt
     1141 aaccgtcgaa atcgtaaact gggacggccg ccagttgagc ctctgtatac agaacctgat
     1201 gccttgaaat cgcttgagta tatacatccg gtaaaatacg gtgatgattt tgaagtaaca
     1261 aagggtgttc gagctgtttt tagggatgcc ggtcatattc ttggatcttc atttatcgag
     1321 ctatcggtta ctgaaaacgg taaaacaaaa aaaatagtat ttagtggcga tcttggtcct
     1381 aaagaccagg caattattcg aaatgccgaa aattttacag gtgccgatta tcttttaata
     1441 gagtctacat atggaaaccg ccttcataaa agtaagcccg atacgtacga agaatttaaa
     1501 cagataatac atgatgctta taatagaaaa ggaaatatag ttattccatc ttttgcggta
     1561 gagcgaactc aagaaataat ctatacattg gctcaacttt tcagatctgg agatattcca
     1621 aaaataccaa catatattga ttcacctctt gcaatatcgg caaccgaaat tttcagagaa
     1681 aatagcgaat gttttgatga tgatactcga aaaatattgg aaagtggcgg atctccgctt
     1741 gattttccta atttacattt gaccagaact acagccgaat ctaaggatct gaccaaaacg
     1801 gctcgcggat ccataattat ctcggcttca ggcatgtgta ctgctggacg tataaaatac
     1861 catcttcaaa gtaatcttta taaacctgat tcaagcatag tttttgttgg ttaccaggca
     1921 gaaggtactc ttggaagaaa acttgtagat ggtgccaagg ttgttagggt atacggagaa
     1981 gatgttgcgg ttaatgcaaa aatacataca cttggtggct tttcgggtca tgcggatcgg
     2041 gaccgtctac tagaatggat gggaacaggt gataacccgg atatgaaagt ttttgttgtt
     2101 catggtgaac acgagtcatc cgaaaatctg gcatctttga ttagagaaaa atacagtttt
     2161 acgactattg tacctacctg gggtgaaata gttgatcttg acacattcga gctaagtaag
     2221 gctaaatacg gtgaacctct cgtggagcat acagacctta aggagcatgg agatgcggca
     2281 attaaagaaa tagaaacatt aatgcaggag tacatttcca aatataaaaa ggtgtcaaaa
     2341 gagtcgccta tggttaagtc tgaggagatc aaatccaatc ttgacgatct taagcagatg
     2401 attaccatgt taaaagatga aatttcgtag atggagtatt tatgcctgcg ctgatagaaa
     2461 caatcactga tcccgcgcat tttcaaaaga tattttacga tttttttcta aagggcgtgg
     2521 tgtatcttaa aacagctaac ggtaatttta aaattcaatt tctgggttat tccgaggggc
     2581 ttattgctct aagaataccc attgtaaaaa atatgccagc gtcgtgtgta atattatgcc
     2641 gaatccctgg tgaaacaatt tatgctcaat tgaagtttgt cgaaaaacag gaggatgagg
     2701 catatatcct taaacctgtc aaaatacaga tgatcagtgt atctcgaaat gaacaacgcc
     2761 attctatatc aggcaaaggc aaacagcttc ttttcgtttc aaaagtaata tctgactcaa
     2821 ttatcgagcg ctccatttct tttgatcaac gcaaagctga attggcaaaa ttgaaaattt
     2881 cagatcagct taaatcggtt tttgtttcca agaagatcta tttctcaaac gaaggctcaa
     2941 gtgatgatag aatgcgttat ttttacaacc gtaaacctga ttatcttatc cccgattttg
     3001 attcaataga tatcaaggat cctgtacaga tgaacttcaa gaatgatatt ttgaataagg
     3061 attattatat tcaaaatcgt cgtgcagtta tgtctgaggc ctctgttgca gtctattatc
     3121 gtgaagcgat accatttggt tacatacagg ttaatcataa taaaaagctt gataaatcan
     3181 nnnnnnnnng ataaatcagt tttaataatg cttaaaaatt tgtctatgca gctttcaaag
     3241 atattcgcta atgttaagct atttaaacct cttgatgaaa atctactggt tagtgatatt
     3301 tcgtctaaag gggtgggaat tgtttttcgg gaaagatcat acattcgttt ttttcctgaa
     3361 aaaagctatg tgcatttcga attgattttg cccgatcaaa tacgcatccc catgcttaca
     3421 gttgttcgaa gcataacttt tcttgaaaat aaaattatca aagccggatg tgaaattatt
     3481 tccatggata aaagcgccat gcagatatat tcacattacc ttcaatcact gtaaagggtt
     3541 gcagttgtat gagacctcta aaattatatc ctttaagtga ttatacctta aaaattgcac
     3601 tattagagga tgacccgata attcttcagg acataaagtc catgcttgtt tccctggggc
     3661 attccgttgt ggctacctat cgttctggtg aagagtttct ggccgagtat cagtttatta
     3721 gatgtgatct ttttattctg gatatcgaac tatatggcaa gaaaagcggt gtcgatgtag
     3781 ctgaagagat tcaaaaatcc agatccgtac cattgatata cctcacctca tattctgatg
     3841 atgaaactgt tacaaacatt cttaaaacag atccttatgg ctttatactt aagcctgcac
     3901 gcgctgagca tcttcggata tccattaata tcgcttatta caagttttta tctgacaaac
     3961 gaataaggga aagtgagcag cgttatcaaa gtattgtaaa aacattaccg cttatgctcg
     4021 cgcgttttaa tcctgttaat gatacggtca ctttctgtaa tgataatttt aaggaccatt
     4081 tttttgatcc gtttgataaa gttcacgccg gtttttttga taaaatgagc gatttgggtt
     4141 ttgaagatct gattgatatt ttcagtagtt taaaccgagt ttcaaacgtt acaattaaag
     4201 aatacgcaat cgcaagtaac ggtgtaacgg ggcattttaa ggtaatctgt caggctctat
     4261 atgattcgga tggtggcctt ttcgaatatc aatttttatg tgaggatatt agtgaagtaa
     4321 gggagcgtga acgaaagata cgtgattaca gtagcgagct tgacaagaaa attcgcgaac
     4381 taaaatgttt atatagtata tctaatattc ttcaaaataa ccgtaatatc agtaaaatgt
     4441 tacagcgtgt agttgatgaa atttctttgg ctatgagtaa tccgtataat gtatcatcat
     4501 gtgttgaata tggagttgac tctttttatt cgtataactt tttttcgagt agcgcaaatt
     4561 ttacgcatcc tattattgtt agaggaagta ttgtcggaca gattcatctt catgtggaaa
     4621 aaaatcagga gcgcgatgat ctttttcaaa agggcgaact tgatctgctt attactgttt
     4681 cagaaatgat tagtaaaatg ctggaaaaaa ccgaggccga aatacagtta aagaaactgg
     4741 aaaaagagat aatcatgacc agtgagagag agaaacagaa tctggggcat gagctacatg
     4801 acggtatcgg acagattctc acaggtgcca gctttatttt gaagtctctg gaaaagaaac
     4861 ttcaaaaaga agcgaatgtt ccacaggagc tgttcgacgt ggtcgattta ataaaggatg
     4921 ctacttcaag atgccgaagg ctctcaaaag ggcttgtccc tgtttcatat tctaatgaaa
     4981 cttttgttta tcttgtagag cagcttcttc acagcaccaa agagttttac aacataactt
     5041 ttgaactatc aatgccagag aattttgatt taaaaaattc atttgtaaaa tctcaacttt
     5101 ttagaattgt gcaagaagcc gtaaataata cggttaagca tgcgcaagcg agcaaaataa
     5161 cactgatact tgaagatgaa aaagagtggg ttcggctggc tgtctcggat aacggtgttg
     5221 cgagggctgt taagggaaaa aatccgggtc ttggcatgaa tatcatgaaa taccgtgctg
     5281 atttgattgg cgctaacttc gatataatta tggaagagaa tgtaggtaca accgttgtcg
     5341 taaggcttcc aggtgatatg gttgtttacg ggcaaaattg aatcaatctg ttattatggg
     5401 ggagtttacg atgaaaataa tgattgttga tgatcatcct gtggttcgaa aaggaataaa
     5461 atcgatactt gaaagtgaag atgatattaa tgttaccgcg gaagcggaaa gctatgacga
     5521 ggctcttgag aaattgggag ttgatcttcc agatttggct attgttgata tagaactaaa
     5581 gggatctgaa aacggggtag ctctaatcaa gtctattaaa gataaatatc cgcaggtcaa
     5641 gactctggtt atgtccatgg atgatggaag tctatatgcg gaaagatcta tacgtgccgg
     5701 agccttgggg tatatcgcaa aggaagaagc ttctgaaaga attgtgagtg caattcacca
     5761 ggttatgtcg ggagaacttt atctgagctc taaactctca aaaatgattg catcgtcaca
     5821 cataatgggt gcaaatgaca aaggaccgga tgttacaact ctttcaaata aggagtttga
     5881 aatttttaag cttattggtc agggttataa acgaagtgaa atatcaaaaa agatgagtat
     5941 gaatataaac actatagagt cgcacaggcg aaaaattagg gaaaaacttg atattaaaaa
     6001 ctctgcagat ctttcacgaa tggcggtcaa atggtacgtt gattctcaat aaagtgtatg
     6061 ttttctgtat tctgaaggat aagttgtatt agagctttac attttaaaaa agtaaacctt
     6121 cgtgtacaac tcacttgaca aaaaagggtt aattcaataa aataatctca gtaatttctg
     6181 cgtataaatt tgattatcgg gagccactga tgacgttttc ctctaaaaat agattttcgt
     6241 cgtttctttc ccgttttaaa cgtgacaaac agacatccag gttaaagaaa aaacttctgc
     6301 tatattttat attggttgcg attgtatccg tttctgtttc tgctcagatt attttagaga
     6361 tgagttctac aaggtttcgt aatcagattc gggatggata tatctcggaa atttcacggg
     6421 agttgggttc ccaggttgct gtaaagcttg agtcagttag tacagatgca attgcggagt
     6481 ctgtttctca gcttcgtaat cgaatgttat taatgcttat tgttgtcagt gcgagtattt
     6541 ttggagcatt ttcacttttt gttcgagata ttgttgttcc gatggatggt cttgtagaag
     6601 ctacaaagaa gattgcctca ggagacttga atgcgcacgc tcctgttatg tcggaagacg
     6661 agattggaca gctcgcagag ctaattaatg atatgaatat aaatatgcag gatctgataa
     6721 cccaggtcag gcgtgaactt gataattata actttgaaat agcctttgct gttgccatga
     6781 taggtcagat tctttcaagc gagaacaccg gggacgccct taaacataaa agacttaaac
     6841 tgtcagaatt taaaagaatt attgagttaa acaagagcgt cgagaatgtt cttgataaga
     6901 tgagtgataa actgacttcg ctgcacagtt ttataagtac ttataccaat tatacaattt
     6961 cttcagaaat tacccagaaa gaaattgatg aatcgttaag ccactacagg tcttaaaaag
     7021 gaacagcaat ggtaatagat tttgttttat ttgagctgaa tatgcaatac tggtacatgt
     7081 tccctgtatc cattttagta gcaattatct gcaactcttc gggtttttca ggaggagtgc
     7141 tttttcagcc gatttataat atttttctga atattcccat tcaaaatgcc gtagcaacag
     7201 gtattgctac cgaaacagtc ggtatgacca gtggtgctct tagatatctt tggtacaaga
     7261 tgattgaatt acctgtcggg ttcacaatga ttatgcttac cattccgggg gttgttttgg
     7321 gaaacttcgc gcttcttgtt atcgatgagg atcttttaaa gctaattttg ggtataatta
     7381 ttgttggttt ggctacaact caattattcg gagctattcg aaaacatttc ggaacaaagg
     7441 aaaacatacc tattgaagat atgtacagct atctttgggt accccctatc ggtggttttt
     7501 tctctgcagc taccggtact ggaatctgcg agcttgctca gcctgttctt gaaaagggtc
     7561 taaaaataaa gacgaaaaaa gcgaacgcaa ctgcaattct tgttgaagca actggtgact
     7621 ggattattac gattcttaat ctgcatgccg ggcttattat gtgggaattg tggattttta
     7681 caggcacggg tgttatactt ggcggacaga ttggtccata tatttcaaaa taccttcccg
     7741 atagggtctt aaagtttgtt ttttcaattt gtgttattgc agtaggtatt ttttatatta
     7801 tcaagggtgt aaactggctg tcaaaataaa atctgttaaa attattatta cggtttttgt
     7861 tgccattaat acgggatttt acgtattttt attacttccc taaatccact tgacgattta
     7921 tccgattatt atactatata aggtgtatca catacataac ttgaggaaat catggaaaac
     7981 ataaaagaga atataggttc ttgccagcag ttatcaattt ctataacatc aactgcaaca
     8041 gatgtaaaca agaaatcaat tgaacgtaag gatcttaccg aagctggacg ggatataatt
     8101 gaacagctaa ttgagcgaaa cgataccatg attgacaatt ttgaaacagt tttgtcaaat
     8161 cttcaggact cacagagggc tatttctaat aacagtgaga atattgttaa caacaaggaa
     8221 atactcgtgt ctattgtaac acggttaaaa accgttggcg gtgagttatc ccaaatggaa
     8281 aacgagattg aaaagcttac tgaaatagtt gatgaaatta agggagatac taacaaaatt
     8341 ttctctttgg ctttaaacgc atc
//
LOCUS       DAOTGZ010000115         8263 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_3064
            NODE_10410_length_8263_cov_6.502924, whole genome shotgun sequence.
ACCESSION   DAOTGZ010000115 DAOTGZ010000000
VERSION     DAOTGZ010000115.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868088
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 8263)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8263)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:22:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,785
            CDSs (total)                      :: 2,751
            Genes (coding)                    :: 2,732
            CDSs (with protein)               :: 2,732
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 12 of 19
            Pseudo Genes (internal stop)      :: 5 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8263
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10410_length_8263_cov_6.502924"
                     /isolate="AMR_MDS_3064"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            334..816
                     /locus_tag="P5123_08435"
     CDS             334..816
                     /locus_tag="P5123_08435"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRX16326.1"
                     /translation="MEITDFRTSIDVEVSRLERIEEYAQKHNMDISDVMNTVLDQYSK
                     KLAKNPVCNDSLNRYHERGTIYKSIHIRFSSISKRKLKDITNLYCYSLSYMLFLAMEF
                     FDELMDGVKEFIRPVFHATFVYNFFGFPYFINCWGMNFYEGQNHSCGQETRKTTFLLC
                     "
     gene            1078..1923
                     /locus_tag="P5123_08440"
     CDS             1078..1923
                     /locus_tag="P5123_08440"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0070064 - proline-rich region binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SH3 domain-containing protein"
                     /protein_id="HRX16327.1"
                     /translation="MKKNIAFFTCIILCGISQLQLSAQNIYNMGSFIKGAKSYTFGDN
                     CRIRKAPSLSAETVDTLSIAMPVTIISKNDKQETISGQTQNWFEISYAKNKKGYIWGG
                     LIAYAYTKDGDYLLLIGITSYSDHSFRGEARLVKNNKLVSKVDFKPHSTDMNKYYGYD
                     ISVKNSSNRGLTSINSLYMIVAGYPACGYTNGTTLLGRSGSELLYLLQTNEVSEAGLF
                     RSSESLIFPSNKDGNKNEIIVHSIMQEYDEKKDDLVTQEEKKSHYKISGKKTTLISEE
                     KIKGI"
     gene            1936..2463
                     /locus_tag="P5123_08445"
     CDS             1936..2463
                     /locus_tag="P5123_08445"
                     /inference="COORDINATES: protein motif:HMM:NF012993.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbon-nitrogen hydrolase family protein"
                     /protein_id="HRX16328.1"
                     /translation="MVLASAQICTADQDIESNLDQHYKLINIAAEKGAQLIVFPEMSI
                     TGYVRETAAENLFTPDDSRLKQLKEISSQKQIIVVAGAPVLINNDLFICSFIIKPDQS
                     VAIYTKQFLHTGEELFFKSSFDYNPYVLLDNEKIYFAICADTNEPFHAQSAAQNGATL
                     YIASICFSKEGLPSG"
     gene            complement(2709..3029)
                     /locus_tag="P5123_08450"
     CDS             complement(2709..3029)
                     /locus_tag="P5123_08450"
                     /inference="COORDINATES: protein motif:HMM:NF025077.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4156 domain-containing protein"
                     /protein_id="HRX16329.1"
                     /translation="MSNRYIALGMFMLIALAGCFSAQLKEGAGVDYVTKKEAPAKCKK
                     IGEVELSASNFEVAISASDVIVKLRNKTAIMGGDFLVVDIIEAESNNAGKYYTGFGRA
                     YKCK"
     gene            3320..4102
                     /locus_tag="P5123_08455"
     CDS             3320..4102
                     /locus_tag="P5123_08455"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012501002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HRX16330.1"
                     /translation="MKKDSMQIIDITPANIADYGVCGYKDVNKHLELRRKIDWYKKYY
                     PKGLRIKGIVSETGGYQGMIEYIPGQYAHRPVAAKDYMFIHCLFVGFKKEYKSQGFAT
                     QLLKLCIDEAKEAGMNGVAVVTRKGSFMAKREIFIKNGFCSVETAKPDFELLVFKFDS
                     DSKNPSFMNLSPDNYPDGITIFRSAQCPYSVKNVDAILKTAEKLKIKAQLIEMDDATA
                     VQNSPCAFGTFCIVKDGKVISHHPISNTRFENIMKEKIVQKN"
     gene            4235..5494
                     /locus_tag="P5123_08460"
     CDS             4235..5494
                     /locus_tag="P5123_08460"
                     /inference="COORDINATES: protein motif:HMM:NF012422.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LuxR C-terminal-related transcriptional
                     regulator"
                     /protein_id="HRX16331.1"
                     /translation="MNTQLFIYIFFLTAAFSSILIFSIYLLKTGPSTWLKYVFFVKTV
                     FLSVCVLIAILIQVSSNQQMVMFWYKIMLTAFLINAALFVHFYSELIIRRKLLLLKGL
                     TYLTAFILIGFLYHIKNPFNSSYFRHNAFWILRESNDTVYFIFFMIFLSFCVSYSIAV
                     LVYWDFHTKNNKEKYSARIISASMVVFFISLLVIDIIMGPSGIITVPHIAPIIISFYV
                     LALCYALFKYHFLFFNLKEYFYEIINNINDLVIFVNPQGKIVTLNDPVQALFSNNYSD
                     LTNHKLTDIVVSSNAFFKEFNALLNTHDKRSYHQRLFLSLSDTEMLDTNGYFSSVFDV
                     HGDFVGVLIIAKENKNIEQFLNDYSLSQRQLELCMLIQLGLTNADIAEKLGITLRTVE
                     SHLLHIYNKVTVSNKIELINIMQQYNV"
     gene            complement(5577..7253)
                     /locus_tag="P5123_08465"
     CDS             complement(5577..7253)
                     /locus_tag="P5123_08465"
                     /inference="COORDINATES: protein motif:HMM:NF014427.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ig-like domain-containing protein"
                     /protein_id="HRX16332.1"
                     /translation="MKSMKTYLHGLLVILLLASVLVIAACGGGGGSDSEKETPATGIS
                     LDITEATIPIGGTKQLTATVTPDNVTDRTVTWTSSNTSVATVSDEGLVTSVALGDATI
                     TASVAGGAFTAECALTVRELEAVITKLNDVTIDSDTNWVICNDVLYFAGYDDDHGREL
                     WKSDGTEAGTVLLKDINSGASGSSPSNFAVVGDLVYFRASDGTHGSELWKTDGTESGT
                     VMVKDIMNGSDASLSYYSYMTAIGEEVYFVAKDGTHGYELWKSDGTKDGTVMVKDINN
                     GDGDSNTKHCVVVGGFLYFSANDGSNGQELWKSDGTEAGTVMVKDIYNGVSSANPINF
                     TVVGNTIFFQAEDGTNGHELWKSDGTESGTFMVKDIYSGTSGSMPSYITAIGNVVYFR
                     ASDANYGSELWRSDGTEAGTVMVKNINTVGTSNPSPNSLTVVGNMLYFLAEESVHGFA
                     LWKSDGTEDGTVLVKDIDSESLTLEIDELTAVGNDLYFTADDGIHGYELWKSDGTEAG
                     TVMVKDYFTTDGYDSYYPKITESNGNIFFLAAETEPHTGYDCNLYVLTWE"
     gene            7596..7829
                     /locus_tag="P5123_08470"
     CDS             7596..7829
                     /locus_tag="P5123_08470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015230041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1902 domain-containing protein"
                     /protein_id="HRX16333.1"
                     /translation="MERVINLHIEKLPEGVFLATSDEVQGLVAQGRTVSETIEIARDI
                     ARKLLEEQNSTNPNPAPQNTLSEKFDYPLIIGI"
     gene            7832..8218
                     /locus_tag="P5123_08475"
     CDS             7832..8218
                     /locus_tag="P5123_08475"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRX16334.1"
                     /translation="MGRLAGFTYRDIIRKLKTLKAMTESYVSLNLDEELLALINDIYT
                     DSRYPSAIGLLPKGKPIPETALLFFSFTAEILEKAVSCLQDSDADRLKISQHSISGLY
                     QYAETQISNRFPRYNCGHRVGRSPYL"
BASE COUNT         2671 a         1671 c         1378 g         2543 t
ORIGIN      
        1 taagtaactg tagaaatatc cttttatttg agaacaaata cttgtacgca ttatcgtgtg
       61 gctttgcttt tccattgcat tttctgacca tccacatcct ccttttttcc atctacatat
      121 aacaactgtt tttttgaaaa aaagggcgaa gaaaactgca attaataatg aataatgaaa
      181 aatgaataat tggtgctact ctacaaatat caactcaaca gatttaatga gtagctctta
      241 gatttcttgc caaataaaaa aaaagataaa aaaaatttaa caaaactacc ttctaaccag
      301 tatacatata tagatttatt ctggaggtaa gaaatggaaa tcacggattt cagaacatca
      361 atcgatgttg aagtaagcag actggaaaga atcgaagaat acgcgcaaaa gcacaatatg
      421 gatatttcag atgtaatgaa tacagttctt gatcaatatt caaaaaaact ggctaagaat
      481 ccagtatgta atgactctct taacaggtat cacgaaagag gtacaattta caaatccatt
      541 catatacggt tttcaagcat ttcgaaacgg aaactaaaag acataaccaa cctttactgt
      601 tacagtttgt cttatatgct ctttcttgca atggaatttt tcgatgaact tatggatggt
      661 gttaaagagt ttattcgtcc tgtttttcat gcaacttttg tttacaattt tttcgggttt
      721 ccatacttca ttaattgctg gggaatgaat ttttatgagg gacaaaacca ttcttgtgga
      781 caagaaaccc gcaaaacaac ttttttattg tgttagaacc ggttttttga taaatctatt
      841 aaaaaaacaa aagcactgaa ctgaaaaacc cattatctca taaaaacaaa tccaacctgg
      901 cacatctgca accaaccaaa aggaagcggc ttgcatcttt atcgtaattt ttttttaaag
      961 acatatttta aaaacatgaa ataacttttt tataaataaa aaacttgtca ctaattatca
     1021 cgacatgtat ataatcatct tattaaaagc taattaacca tacaaatcgg agagaccatg
     1081 aaaaagaata ttgccttttt tacctgtatc attttatgcg gcatttcaca actacaactg
     1141 tcagcacaaa atatctataa catgggttct ttcatcaaag gagcaaagag ctataccttt
     1201 ggtgacaact gccgcattag aaaagctcct tcactatcgg ccgaaaccgt tgacacactt
     1261 tcaattgcaa tgcctgtaac aataatcagc aaaaacgata aacaggaaac cataagcgga
     1321 caaactcaga actggtttga gatcagttac gctaaaaata aaaaagggta catctggggc
     1381 gggcttatag catacgcata cacaaaagat ggcgattatc ttttactcat cgggattacc
     1441 tcgtatagtg accatagttt taggggagaa gcaaggctgg ttaaaaacaa caaacttgtt
     1501 tcgaaagttg actttaagcc tcatagcacc gacatgaata aatactacgg ctatgacatt
     1561 agcgtaaaaa atagttctaa tcgtggcctt acttcaatta attctctgta catgattgtt
     1621 gccggttatc ctgcctgtgg ttacacaaat ggaacgactc ttcttggccg atccggtagt
     1681 gaattacttt atcttttaca aacaaatgaa gtttccgaag ctggattatt cagaagtagc
     1741 gaaagcttga tttttccgtc taacaaagat ggtaacaaaa acgaaataat cgttcactca
     1801 ataatgcagg agtatgacga aaaaaaagac gacctggtaa cacaagaaga aaaaaaatcc
     1861 cattacaaga tatctggcaa aaagaccacg ctaatatcag aagaaaaaat aaagggaata
     1921 taaagaggca aattgatggt actcgcatca gcgcaaatat gtacagccga tcaagatatt
     1981 gaatcaaatc ttgaccaaca ttacaaactg attaatatcg cagcagaaaa aggcgctcag
     2041 cttattgtct ttcctgaaat gtcaattacc ggttatgtac gtgaaaccgc tgccgaaaat
     2101 ttatttaccc cagatgacag cagactaaaa cagttaaaag agatctcctc tcaaaaacag
     2161 attattgttg ttgcaggagc acctgtttta attaataacg acctgtttat ttgcagcttt
     2221 ataattaaac cggatcaatc agtagcgatc tatacaaaac aatttctgca taccggagaa
     2281 gaactttttt tcaagtcatc tttcgattat aatccttatg ttttgcttga taacgaaaaa
     2341 atatatttcg cgatttgcgc ggataccaat gagccctttc atgcacaaag tgctgcgcaa
     2401 aacggagcaa cactatacat cgcgtcaatt tgcttttcca aagaggggtt gccttccgga
     2461 taaataactc tgtcaaacta tgctaaaaac cacgacatga atgttttaat ttcaaatttt
     2521 tgcggcaagg tttacaggta tcagtgcggc gggcaaagcg gtttctggga caataaaggt
     2581 aatcttgtct ccggattaaa cgacacagac aacggtttat taattataca aaaacatgaa
     2641 agcagctgga ctggatattc cttggtagtg taattacatg attttaatag caaaatcgtg
     2701 aatatcatct atttacactt atatgcgcgt ccaaacccgg tataatattt accggcatta
     2761 ttactttcag cttcgataat atctactacc agaaaatcgc cgcccatgat tgcggtttta
     2821 tttcgtaatt ttacaattac atcacttgca ctaattgcca cttcaaaatt ggacgcgcta
     2881 agttcaactt ctccaatttt tttacattta gcaggcgctt ctttctttgt aacataatct
     2941 acccctgcac cctcttttaa ctgtgcagaa aaacaacccg ccaaagcaat aagcataaac
     3001 attccgagtg caatatatct attactcata attttaatat cctcacttta cttaatcaaa
     3061 ttacaataat gaaaacgcaa taatttgtca attcttaata caaaaccagt cttttttaga
     3121 gtcatttgta attttttatg gaaataatta tttgatcaga ttcattgttc acataagatt
     3181 ttagtatgga acagaattaa acagaatcat ctgtttagat tatttgcaag tcacttacga
     3241 aagcaatatc gcaatcttaa catgtattca gccattatag tcaaaaggtc tttctgataa
     3301 aatacaagga gtaccattta tgaaaaaaga ctcaatgcaa attatcgata taactcccgc
     3361 caatattgcc gattacggag tctgcggata taaagatgtt aacaagcatc tggagcttcg
     3421 aagaaaaatt gactggtaca agaagtacta cccaaaagga cttcgcatca agggcattgt
     3481 ctcggaaacc ggtggttatc agggaatgat tgaatacatt ccgggccaat acgctcaccg
     3541 tcctgtcgcg gccaaagact atatgtttat tcactgtctt tttgttggat ttaaaaaaga
     3601 gtacaaaagc caaggatttg ccactcagct cttaaaactg tgcatagatg aagcaaaaga
     3661 agcagggatg aacggtgttg ctgtagttac aaggaagggt tcgtttatgg caaaacgcga
     3721 aatatttatc aaaaatggat tttgctctgt agagacagcg aaacctgatt tcgaactact
     3781 agtttttaaa tttgattctg acagcaagaa tccctcgttt atgaatcttt ctcccgataa
     3841 ttatcccgat ggcataacaa tattcagatc agcacaatgt ccctactccg tcaaaaatgt
     3901 ggatgcgata cttaaaacag ccgaaaaact taagatcaaa gcccagctta tcgagatgga
     3961 tgatgccaca gccgtacaaa attcaccctg cgccttcggt acattttgca ttgttaagga
     4021 cggaaaagtg atcagtcatc atcctattag caacaccaga tttgaaaata ttatgaagga
     4081 aaaaatagtg caaaaaaatt gacaagttag cacaatttat cattatttgt ttaaattatg
     4141 taattttttt tgggtacttt gtcattccca atcaataaaa tatagtattg tcgtgtagcg
     4201 ttaatcagat atctacttaa agagaagaca tctcatgaat acccaacttt ttatctatat
     4261 ctttttttta acagccgctt tttcatccat actgatattt tcaatatatc ttctaaaaac
     4321 aggtccttct acatggttaa aatatgtttt ttttgtaaaa acagtttttc tttcggtatg
     4381 tgtgctaatt gcaatattaa tacaagtatc atcaaatcaa cagatggtta tgttctggta
     4441 caagatcatg cttacggcat ttctgattaa tgctgcgctg tttgttcatt tttattccga
     4501 acttataata cgccgaaagc ttcttttgtt aaaaggatta acatatctta cagcttttat
     4561 tttaatcggg tttctttatc acatcaaaaa tcctttcaat tccagctatt ttcgccacaa
     4621 cgccttctgg atccttagag aatcaaacga tacagtgtat tttatttttt ttatgatctt
     4681 tctatccttt tgcgtttctt actctatcgc agtacttgta tattgggatt ttcacacaaa
     4741 gaacaataaa gaaaaatatt cagcccggat tatttctgcc tcaatggttg tttttttcat
     4801 ttctcttctt gtaatagata tcattatggg gccgtcagga attattacgg ttccgcacat
     4861 tgctccaata ataatctctt tttatgtttt ggctctctgt tatgccctgt ttaaatacca
     4921 ttttctgttt tttaatctta aagagtattt ctacgaaatt attaataata tcaatgatct
     4981 tgtaatattt gttaaccccc agggtaaaat agtaacacta aacgaccccg tgcaagcttt
     5041 gttttctaat aattattctg atcttacaaa tcacaaattg acagatattg ttgtcagttc
     5101 aaatgctttt ttcaaagaat tcaatgccct gcttaatact catgataagc gttcttatca
     5161 tcagcgcctt ttcctgtctc tttcggatac cgaaatgctt gacacaaacg gatatttttc
     5221 gtcggtattt gatgttcacg gtgattttgt aggcgtactg ataattgcaa aagagaacaa
     5281 aaatatagaa cagtttctaa acgattatag tttaagccag cggcagcttg aactctgtat
     5341 gttaattcaa cttggtttaa cgaatgccga tattgccgaa aaacttggca tcacacttcg
     5401 aacagtcgag tctcatctgc ttcatattta taataaggtt acggtcagca ataagattga
     5461 gcttataaac attatgcagc agtataatgt ataaaggcgc ttgcttaata tgcctgtaaa
     5521 aagttacgct gccttactta atgacagcgt aacttggcgg aatagtattt ataccactat
     5581 tcccatgtca gcacatacag gttacaatca tatcctgtat gtggctctgt ttcagctgca
     5641 aggaagaata tgttgccgtt actttcagtt atcttgggat aataactatc gtaaccgtct
     5701 gtggtgaaat agtctttaac cattacggta cctgcttctg ttccatcgct cttccacaat
     5761 tcgtatccat gaataccgtc atcagctgta aaatagaggt cattaccaac cgctgttaac
     5821 tcgtctattt ctaaagtaag actttcactg tcgatatctt taaccagaac tgtaccgtct
     5881 tcggttccat cacttttcca caatgcgaat ccgtgcacac tttcttctgc aagaaaataa
     5941 agcatattac caaccacagt taaagaatta ggcgatggat ttgaagtgcc cacggtattt
     6001 atatttttta ccataaccgt tccggcttct gttccgtcac ttcgccatag ctctgaccca
     6061 taatttgcat cactagctct aaaatagacc acattaccaa ttgctgttat ataagatggc
     6121 attgaaccgg aagtaccact atatatgtct ttcaccataa aggttcctga ttcggtacca
     6181 tcacttttcc ataactcatg tccattagtc ccgtcttcag cctgaaaaaa aattgtgtta
     6241 ccaactacag taaagttaat cggattggct gacgaaacac cattataaat atccttaacc
     6301 ataacagttc ccgcttcggt accatcactt ttccataact cttgaccatt ggaaccatca
     6361 ttagcgctaa aataaagaaa accaccaact acaacacagt gttttgtatt cgagtcacca
     6421 tcaccattat ttatgtcctt aaccataaca gttccatctt tagtaccatc acttttccat
     6481 aactcataac catgggtgcc gtctttggct acaaaataga cctcttcacc tatcgcagtc
     6541 atataactgt aatatgaaag tgaggcgtca ctaccgttca tgatatcttt gaccataacg
     6601 gtaccggact ccgtaccgtc agttttccat aattcagaac catgggtacc atcgcttgct
     6661 ctgaaataaa ccagatcacc aactacagcg aaatttgagg gagatgatcc cgaagctcca
     6721 ctattaatgt cctttagtag cacggtacca gcttcagtgc cgtcactttt ccataattct
     6781 cttccatgat catcgtcata tcccgcaaaa tagagcacat cattacagat tacccaattc
     6841 gtgtcagagt ctattgtaac atcgttcagt ttcgttatta ccgcttcaag ctcacgcaca
     6901 gtcagcgcgc actcagccgt aaatgcgcca cccgcaacac tggctgtaat tgttgcatca
     6961 cccaaggcta ccgatgttac cagaccttca tccgacactg ttgcaacaga tgtgttgctg
     7021 cttgtccatg ttactgttct gtctgttacg ttgtcgggcg tcactgttgc ggttaactgc
     7081 ttggtgccgc ctataggaat tgttgcctct gtaatatcaa gcgatatgcc tgttgcgggt
     7141 gtctcctttt ctgaatctga tcctccaccg cctccacagg ctgcgattac caaaaccgag
     7201 gctaataaaa ggattacaag taatccgtgc agatacgttt tcatactttt catgcgaact
     7261 tttctccatt aatattttta gaaaccggca ataccggttt ccctgtaaaa taagggaaaa
     7321 gtatagccca aaacgccgga tttgccatcc gtgtttttac gcacgttgca ataaaaactg
     7381 ctcttttttt ggaaaaaagt acttattttt gggggttatc gtggtatttg gtaaaaaata
     7441 gcttctcaga atgttcgctt gagcggcaaa gggaattccg aagttcccgg gcacaaattt
     7501 taatttatag agattctcca aatataattt gacatatttc aaaaaacatt caatatacta
     7561 atagattgaa aagatttatt ataaacgaag gtactatgga acgagttata aatctgcata
     7621 ttgaaaaact accggaagga gtctttcttg caacttctga cgaggttcaa ggacttgtcg
     7681 cccagggaag aaccgtcagt gaaacaattg aaatagctcg agatatcgcg cgaaaactgc
     7741 tcgaagaaca aaacagtact aaccccaatc ccgcaccaca aaatacatta tcagaaaagt
     7801 ttgattaccc tcttatcatc ggcatataac aatgggaagg ctggccggct ttacgtacag
     7861 agatattata cgaaagctca aaacattgaa agcgatgact gaatcgtatg tatcgctcaa
     7921 ccttgatgaa gagctgcttg cgctcataaa cgatatatat accgattccc gatacccttc
     7981 agctataggt ttattaccaa aaggaaaacc aattcctgaa acagctctac tattttttag
     8041 ttttacagcg gaaatccttg aaaaggcagt tagctgcctg caagactcag atgccgacag
     8101 attaaaaatc tcgcagcact caatatcggg gctgtaccaa tatgctgaaa cccaaatatc
     8161 caaccgtttc cctcgataca attgcggtca tcgagtagga cgaagtccgt acttgtagtg
     8221 agtttatcga actatcgaaa tgcggcaatc acacttcgac aag
//
LOCUS       DAOTGZ010000116         8258 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_3064
            NODE_10418_length_8258_cov_5.184445, whole genome shotgun sequence.
ACCESSION   DAOTGZ010000116 DAOTGZ010000000
VERSION     DAOTGZ010000116.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868088
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 8258)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8258)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:22:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,785
            CDSs (total)                      :: 2,751
            Genes (coding)                    :: 2,732
            CDSs (with protein)               :: 2,732
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 12 of 19
            Pseudo Genes (internal stop)      :: 5 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8258
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10418_length_8258_cov_5.184445"
                     /isolate="AMR_MDS_3064"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(140..1135)
                     /locus_tag="P5123_08480"
     CDS             complement(140..1135)
                     /locus_tag="P5123_08480"
                     /inference="COORDINATES: protein motif:HMM:NF014206.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009428 - bacterial-type flagellum basal
                     body, distal rod, P ring [Evidence IEA];
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body P-ring protein FlgI"
                     /protein_id="HRX16335.1"
                     /translation="MKIRTVVFLCGIFVFSFISAQVSVKVGDISYIDGLKENQVFGYG
                     LIVGLQGTGDSRINVTKNSLRNLLKNLGLEEDDVEKSKNVAAVLVTASLPEMVRVGDR
                     IDVTVSSIGDAKSIEGGMLVQAPLKGADGNTYIVAQGRVSSVESGRAGRKLRTTALIS
                     RGGIVERDIETVFSTENMVTLVLNEWDFDLANQIVENVLAEYPDSRPSINQNGKITFT
                     IPENVNFSIFIARVQDMEVTPDYGPRVVINEKEGTIVMGGSIKVSESVVSKDGLTISV
                     RGEKQPVGAALIKESTTVKDVVEALNYAGLEASDIVSVLKALRDAGALHAELIIK"
     gene            complement(1132..1755)
                     /locus_tag="P5123_08485"
     CDS             complement(1132..1755)
                     /locus_tag="P5123_08485"
                     /inference="COORDINATES: protein motif:HMM:NF014195.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009427 - bacterial-type flagellum basal
                     body, distal rod, L ring [Evidence IEA];
                     GO_function: GO:0003774 - cytoskeletal motor activity
                     [Evidence IEA];
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body L-ring protein FlgH"
                     /protein_id="HRX16336.1"
                     /translation="MKKFFITLFFLTTVLSAQSLWRESNLYSPRANLVAGETIVVIID
                     DVSRMRYTLNVENSKSSSVNSVPDTSITAYLPPVNSNRNIKHNDGVKVESRGNLALRL
                     AVTVNNRMDDGTYNVTGAKTYTFNGVSTRMSITGRVHPSSITGGAIRAEKVVNFALNI
                     SSATQGLGLNLQRTLEEDESATAEMTEEQKQRIITDYLQKMVDELTK"
     gene            complement(1752..2255)
                     /locus_tag="P5123_08490"
     CDS             complement(1752..2255)
                     /locus_tag="P5123_08490"
                     /inference="COORDINATES: protein motif:HMM:NF024544.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagella basal body P-ring formation protein
                     FlgA"
                     /protein_id="HRX16337.1"
                     /translation="MKTILIMLVLLTSSVSFGQVKVFLHSSLKTDGKNVSLSDIARIG
                     GTQNGAIEIDEIMIEQKHLENGYISRKTVQTILQENGITGVVYGTSVRLLPIDKKNGS
                     VNKSSLVDRGDIVDIIIRRNGILIETTGTSLVKAKPGDKVTVKVNSKGRRLKGILLEN
                     RKVEVGG"
     gene            complement(2255..3049)
                     /gene="flgG"
                     /locus_tag="P5123_08495"
     CDS             complement(2255..3049)
                     /gene="flgG"
                     /locus_tag="P5123_08495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010570244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009426 - bacterial-type flagellum basal
                     body, distal rod [Evidence IEA];
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal-body rod protein FlgG"
                     /protein_id="HRX16338.1"
                     /translation="MMRSLWTAASGMITQQFNIDTVSNNLSNVNTTGFKKQRAEFEDL
                     LYQTVLLAGTPATEVTEIPTGIQVGHGAKVSATQKMFTQGSLQQTENLTDIALEGEGF
                     FKVRLYDGTEAYTRDGSFKMDSNRQIVTSNGYFLDPPVILPDEFQRETLNISQDGRIT
                     VKLIGDDDIVDVGQIELYRFVNPAGLQSIGQNLFKVTGASGEEIPGQPALNGMAKTHQ
                     GFLEMSNVKVVEEMVNMIVAQRAYETNSKAIQTSDSMLGTAIGLKR"
     gene            3456..4217
                     /gene="hisF"
                     /locus_tag="P5123_08500"
     CDS             3456..4217
                     /gene="hisF"
                     /locus_tag="P5123_08500"
                     /EC_number="4.3.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018248755.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000107 - imidazoleglycerol-phosphate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="imidazole glycerol phosphate synthase subunit
                     HisF"
                     /protein_id="HRX16339.1"
                     /translation="MLAKRVIPCLDVKDGRVVKGVNFVDLIDAGDPVENGAFYDSEGA
                     DELVFLDITASSDKRAIILDMVKKVAETVHIPFCVGGGVRTIDDVRLLLENGADKVSI
                     NSAAVKNPDLIHEASRRFGSQCIVVAIDAKRNETGGWTVYLNGGRTATELDALEWGIK
                     AQDLGAGEILLTSMDRDGTKKGYDTKITSLFSSTLKIPVIASGGVGNLEHLYEGFSKG
                     KADAVLAASIFHFREYSIKEAKEYLLKQGIPVRMI"
     gene            complement(4226..5293)
                     /locus_tag="P5123_08505"
     CDS             complement(4226..5293)
                     /locus_tag="P5123_08505"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRX16340.1"
                     /translation="MENLDNAINSLSDQQKKYDIALHFTGNDQDKAKRMVSNSYFDLV
                     AVKGSFSSSSLYGAFIIFFNIEYAKVEHTVFVVSPDYQLQSLVIKNDWKSLEKEILNS
                     RTSSAASRINADFSDKFKKGFSLSLGRDFARYLGKSDMTQVTHMIQRFMQESTELKRI
                     EVSVEIQNMSSLQLELESLTSVKISGKLNARKKAEEAEAEQNQPADTDPVAGKDGVRL
                     VILSTVVLSPIKGKYISNVAPGDKIMVSMIETTPQVLEIAKAFNAYDSESKKISSIPA
                     RVKSIEYIDGIGYKIFAVIAKGIIAKIIEEETSIKVALDPATVLINEDESDKGGIGIP
                     IIIGLTAAIVMLILFVIVVVL"
     gene            complement(5307..5750)
                     /locus_tag="P5123_08510"
     CDS             complement(5307..5750)
                     /locus_tag="P5123_08510"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HRX16341.1"
                     /translation="MRSKHDFPSVNTKTPDGIKPNGIPYKVVVIEPHEFLRKQIIQIL
                     ESEGYEILASGKDGQDGVDIMERIGSEVDIITTDLDMPRLDGYAFLYNVKEKNYKAKV
                     VFISNDTTKGVLKDLLTMGASDFILKPIKRIRLLERMKMLTDKIK"
     gene            6212..7705
                     /locus_tag="P5123_08515"
     CDS             6212..7705
                     /locus_tag="P5123_08515"
                     /inference="COORDINATES: protein motif:HMM:NF020252.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HDOD domain-containing protein"
                     /protein_id="HRX16342.1"
                     /translation="MNIKPKQKIECSTNDISGSIKRKDQYYIQFAHFSKDVEIFFHKL
                     LTRTLASYDLLYLKSGISTILKELITNATKANIKRLYFTQKGLDISQKADYISGMDTF
                     KEDCLRENAPILDQLEKSNFRVRILFLPEGDLFSVKIINNIQIAPEELKKIEARISKA
                     YKYNDITDAFNDVLDDSEGAGLGLIMAMLIFKNSGFEKKDFSITSGPKQTVCTIRISN
                     TSNKIRTREIAKEIIDDVEYLPSFPENIITLTELCDTPDSTIQEISNYVKKDPSLAAS
                     ILKQANSVGYLTSNKVTTIEEAIMKIGLAGIKALAIAASIEKIVDERYSQYKDLWETS
                     YRKAFYAYKIAIQLKDRKLAEKAYLSSLLSKIGEIALLSAKKLSMERINKIAGIKEIT
                     SIDLLQEISLGISHPTLSAMITEKWNFDSELTNAIKYHLRPHMAPKGIKELIYIIYLA
                     HILIEFEHRNILYQSLDFDVLKFFNLKTEEELKKLHSILHQAYSKNM"
     gene            7801..>8258
                     /locus_tag="P5123_08520"
     CDS             7801..>8258
                     /locus_tag="P5123_08520"
                     /EC_number="6.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006918929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004820 - glycine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006426 - glycyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine--tRNA ligase subunit alpha"
                     /protein_id="HRX16343.1"
                     /translation="MYFQDLIATLNEFWSKQGCINIQGYDTEVGAGTFNPGTFLRALG
                     PEPWNVSYVEPSRRPTDGRYGDNPNRLQHYYQYQVIMKPSPENIQDLYLDSLKAIGLN
                     LNEHDVRFVEDDWESPTLGAAGLGWEVWLDGMEITQFTYFQLCGSLELSP"
BASE COUNT         2617 a         1722 c         1344 g         2575 t
ORIGIN      
        1 gctatcccat agtttctgat caagaggact ctcattttta cttttgctct ttctagtcat
       61 actatccata atctgccggc cctttatatg tgcaaactgc tctgttccta ttccctgtat
      121 catataaatc tcctgatgat tattttatta ttaattctgc gtgaagagcg cccgcgtcac
      181 gaagcgcctt taaaaccgaa acaatatcac ttgcttcaag tccggcataa tttaatgctt
      241 caacaacatc ttttacagta gttgattctt ttataagagc tgcccctact ggctgtttct
      301 ctccgcgaac cgatatggtt aagccgtctt tagagacaac ggattcagat accttgattg
      361 atccacccat aacgatggtt ccctctttct cgtttatcac cactcttggt ccataatcag
      421 gagttacttc catatcctga accctagcaa taaatattga aaaattcaca ttttcaggta
      481 tggtaaatgt tattttacca ttttggttta tcgatggacg cgaatcagga tactcagcaa
      541 gaacattttc tactatctga ttggccaaat caaaatccca ctcatttaat acaagtgtta
      601 ccatattctc tgtagagaaa accgtttcaa tatcacgttc aactattccg cctcgtgaga
      661 taagcgccgt tgtacgaagt tttctcccag cccttccaga ctctaccgac gaaacccttc
      721 cctgtgctac gatatatgta ttaccatcgg ctccttttaa tggagcctgt acaagcattc
      781 cgccttcaat tgattttgca tcaccaattg atgatacggt tacatcaata cgatctccaa
      841 ctctcaccat ttcgggtaat gaagctgtca caagtactgc ggcaacattt ttagattttt
      901 ctacatcgtc ttcttcaagc ccaaggtttt ttaagagatt gcgtaatgaa ttttttgtaa
      961 cgtttattcg tgagtcgcca gttccctgca aaccaactat cagcccataa ccaaaaacct
     1021 gattttcctt taaaccatca atatatgaaa tatcgcctac tttgactgat acctgtgccg
     1081 aaataaatga aaaaacaaat attccgcaca agaacactac tgttctaatt ttcattttgt
     1141 taattcatcc accatttttt gcagatagtc cgtaattata cgctgctttt gctcttcggt
     1201 catttcagcg gttgcactct cgtcttcttc aagcgtacgc tgtaaattta acccaagtcc
     1261 ttgcgttgca ctactgatat tcaaagcaaa attcactacc ttttcagctc taattgctcc
     1321 acctgtgata ctactaggat gcacacgacc tgttatactc attctagtgc ttacaccgtt
     1381 aaatgtataa gtttttgctc cagtaacatt atatgttcca tcatccatac gattatttac
     1441 tgttaccgca agacgcagag caaggttccc tctgctctca acttttactc catcattgtg
     1501 ctttatgttt cgattactat ttacaggtgg aaggtatgcc gttatggatg tatccggtac
     1561 agagtttact gatgaacttt tactgttttc aacattaagg gtatacctca tacgtgaaac
     1621 atcatcaata attacaacta ttgtctctcc agcaactaaa ttcgctcttg gagaataaag
     1681 attgctttcc cgccataatg actgggcgga taaaacagtc gttagaaaaa ataacgttat
     1741 aaaaaatttt ttcatccgcc gacctcaacc ttcctgttct ctaacaatat accttttaag
     1801 cgtctccctt tactgttaac ctttacggtt accttatctc caggctttgc ctttacaagg
     1861 gatgtaccag tagtctctat cagtattcca tttcgcctaa taataatatc tacgatgtca
     1921 cccctgtcaa caagagagct tttattaact gatccatttt ttttatcaat aggaagtaac
     1981 ctgacagatg ttccataaac aacaccggtt ataccgtttt cctgtaaaat cgtctgaaca
     2041 gttttacgac taatatatcc attttccaag tgcttctgtt ctatcattat ctcatcaatt
     2101 tctatggctc cgttttgcgt tccaccaatt cgggctatat ctgacaaaga tacattttta
     2161 ccatctgttt ttaatgatga gtgaagaaat actttaacct gcccgaaaga gacagagctt
     2221 gtcaatagaa caagcattat cagaatagtt ttcattaacg ttttaaacct attgctgttc
     2281 caagcataga atccgaagtc tgaatggctt ttgaatttgt ctcgtatgca cgttgcgcga
     2341 ctatcatatt aaccatctct tcaactacct tgacattaga catttcaaga aaaccctgat
     2401 gggttttagc cataccgttc aatgccggtt gaccaggtat ttcttcacca gaagctccgg
     2461 tgactttaaa tagattctgg cctatactct gtaatccggc tgggttaaca aatctgtaaa
     2521 gctctatctg tcctacatca acaatatcat catctccaat cagtttaacg gttattcgtc
     2581 catcctgact tatattcaag gtttctcttt gaaactcatc aggcaatatc actgggggat
     2641 caagaaaata tccgttagaa gtcacaatct gcctgttaga gtccatttta aatgagccgt
     2701 ctctggtata agcctcagtg ccatcataca agcgaacctt aaaaaaacct tcaccttcaa
     2761 gcgctatgtc agtaagattt tctgtctgct gcaatgatcc ctgagtaaac atcttctgtg
     2821 ttgccgaaac cttggctcca tgtcctacct gaattccagt aggtatctcg gtaacttcag
     2881 ttgctggagt tcccgcaaga agtaccgttt gatacaaaag atcttcaaat tctgcacgct
     2941 gttttttaaa tccagttgta ttaacatttg ataaattgtt ggatacagta tcaatattaa
     3001 actgctgcgt tatcatacca gacgcggcag tccacagtga tctcatcata tcgtacctct
     3061 caacggttat tattaagcaa ttaccgttat tcaataattt tgattaaaaa acctggtgtg
     3121 aataaaataa taagaaccgt acaataacag tcgttaataa attttcatac aaataaacca
     3181 gattccctat ttaaatactt cggtatattt gaaaaatagt ttagataatc tctaaaaaaa
     3241 tcaccttatg tccctttaca acaaatgtat tatacatcta taatcaaaga agcactctgt
     3301 tggttaatct tttgaatttt ttccataaat atgagttgat ttataccact ctaatggttt
     3361 ttaagtaata tttttaatag taataaactt gcaatactag attattgctc attatcttgt
     3421 aaaatttaac atatccaata gacaacggag atatcatgct tgcaaaacga gttattccat
     3481 gccttgacgt aaaagatggg cgcgtagtta aaggtgtcaa ttttgttgac ctcattgatg
     3541 ccggcgatcc agtggaaaat ggagccttct atgactctga aggcgccgat gaacttgttt
     3601 ttctcgatat aactgcctcc tcagataaac gtgcgataat acttgatatg gttaaaaaag
     3661 tagcagaaac agtgcatatc cccttttgcg tgggtggtgg tgttcgaaca attgacgatg
     3721 ttagattatt gctggaaaac ggagcagata aagtatctat aaacagcgcg gctgtaaaaa
     3781 acccggatct gattcatgag gcatcacgcc gctttgggtc gcagtgtata gtcgtagcca
     3841 tagacgcaaa acgcaatgaa acgggaggtt ggaccgtata tcttaacggt ggacgaaccg
     3901 ctaccgagct tgatgctctt gaatggggaa taaaggctca agatcttgga gccggagaaa
     3961 tattactgac aagtatggat cgagacggca caaaaaaagg ctatgacaca aaaatcacat
     4021 ctttattttc atcgactctc aaaatacccg taatagcctc tgggggcgtt ggcaatcttg
     4081 aacatttgta cgaagggttt tccaaaggta aggcagacgc agtactggca gcatctattt
     4141 tccatttcag agaatactca atcaaagaag caaaagagta tctattaaag caaggcattc
     4201 cagtaagaat gatctgatta agtctctata aaaccacaac aatcacaaaa agaataagca
     4261 taacaatggc agctgtgagt cctattataa tcggtatgcc tataccgccc ttatcagatt
     4321 catcctcatt tattaagacc gtagcaggat cgagcgctac ttttatactt gtctcttctt
     4381 caattatctt cgcaataatt ccttttgcaa taacagcaaa aatcttatat cctattccat
     4441 caatatattc aatagacttg acacgagctg gtatcgaaga tattttcttt gattcagaat
     4501 cataagcatt aaacgctttg gcaatttcaa gaacctgagg agtggtttct atcatagaaa
     4561 ccattatctt gtcacccgga gcgacatttg agatatattt accttttatt ggggacaaga
     4621 ctacagtact gagtataaca agacgaacac cgtctttgcc ggccacagga tctgtatccg
     4681 caggttgatt ctgttctgct tcagcctctt ccgctttttt tcgagcatta agctttccac
     4741 ttattttcac actggttaat gattcaagtt caagctgtaa tgaagacata ttttggatct
     4801 ctacagacac ttctattcgc tttaactctg tcgattcctg cataaaacgt tgtatcatat
     4861 gagttacttg ggtcatatca cttttgccta gataacgggc aaaatccctt cctaaagaca
     4921 aagaaaaacc ttttttaaac ttatcagaaa aatctgcatt tattcttgaa gcagcggaag
     4981 aagttcgtga atttagaatt tccttttcaa gacttttcca atcatttttt ataacaagcg
     5041 actgaagttg ataatcaggt gaaacaacga aaactgtgtg ctctactttc gcatattcaa
     5101 tgttaaaaaa aataataaac gcaccataaa gtgaagaaga actaaaagat cctttcactg
     5161 caacaagatc aaaataggag ttagaaacca tacgtttcgc cttatcctga tcattgcctg
     5221 taaaatgaag tgcgatatca tactttttct gttgatccga aagcgaattt atagcattat
     5281 ccaaattttc caaaataacc ccgaccctat tttattttat ctgttagcat tttcattctt
     5341 tcaagcaacc ttattctttt gattggctta agaataaaat ctgatgcccc catggttaat
     5401 aaatctttaa gaaccccctt ggtagtatcg ttactgataa aaacgacctt agctttatag
     5461 tttttctctt ttacattgta aagaaaggca taaccatcaa gtctgggcat atcaagatcc
     5521 gtagtaataa tatcaacctc tgaaccaatt ctttccataa tatcgacgcc atcctgaccg
     5581 tcttttccgg aggccaaaat ttcgtatcct tcagattcaa ggatctggat aatttgcttt
     5641 ctcaaaaatt catgaggctc aataaccaca accttgtagg gtatcccgtt aggtttaatt
     5701 ccgtccgggg tttttgtgtt aacagaaggg aaatcgtgct tgcttctcat gcgcgttgct
     5761 ccttaatcat taactgttaa aaccagattc caattatata tcaaaaacct aaaatcagtt
     5821 caaacagtag attccgaaaa taatgtccat attaataatc acgttatcag ataaacaaaa
     5881 acatcaccta taaagcccag caaggctcat ttcgaaacct taaaacatca aaatgaaaca
     5941 aatactaccg atacttcaaa aaaaacagtg aaattgtcaa cataattatt gcctctaaaa
     6001 cagtatagtt tacgataggt caagcaaaag ctttttacat ggttatggag gttcaatgag
     6061 ttgctgtttt attttataca acttaaatat ttatttacaa aatcataacc ttcaaatatt
     6121 actattagac agttttttag atggatattt tactagctca aaaaataatt ttagtatata
     6181 attatatatt ggaacctaac ggaaaaaatc aatgaatatt aaacctaaac agaaaattga
     6241 gtgtagcact aatgacattt ctggttcaat aaagcgaaaa gatcaatatt atattcaatt
     6301 tgcgcatttt tcaaaagatg tcgaaatatt ttttcataaa ttattaacaa gaacattagc
     6361 ctcttatgac ctgttatatt tgaaaagcgg tatttctaca atacttaaag aactcattac
     6421 caatgcaaca aaagcaaata ttaaaagact ttattttaca cagaagggtc ttgatattag
     6481 ccaaaaagcc gattatatct ctggaatgga cactttcaag gaagattgtc ttcgagaaaa
     6541 tgcccctata cttgatcaac ttgaaaaaag caacttccgt gtacgaatac tattcttacc
     6601 cgaaggagat ctgttttcag ttaaaattat taataatatc caaattgcac ctgaagaatt
     6661 aaagaaaatt gaagcgagaa tatccaaagc ctacaaatat aacgatatta ctgatgcttt
     6721 taacgatgtt cttgatgact ccgaaggagc gggcctgggt cttattatgg caatgctaat
     6781 atttaaaaat agcggctttg aaaaaaagga tttctccata acttccggcc caaaacagac
     6841 cgtatgcact ataagaatat caaatacctc aaataaaatt cgaactcggg aaatagcaaa
     6901 agagatcatc gacgatgttg aatatcttcc ttcatttccc gaaaatataa taactttaac
     6961 ggaactttgc gatactccgg actcaaccat acaggaaata tcaaactatg ttaaaaaaga
     7021 tccatccctc gctgcctcga tattaaaaca ggccaactca gtcggctact taacctctaa
     7081 caaggttacg acgatcgaag aagcaatcat gaagatcggt ctggccggta tcaaggctct
     7141 tgcaatagcg gcaagtattg aaaaaattgt cgatgaacgc tattcacagt ataaagatct
     7201 ttgggagact tcatacagaa aggcatttta cgcttataaa atcgcaatac aactaaaaga
     7261 tcgaaaactt gcagaaaaag catatctttc atccttgcta tcaaaaattg gtgaaattgc
     7321 actgctatcc gctaaaaagc tatcgatgga acgtattaac aagattgccg gaataaaaga
     7381 gataaccagc atagatcttc tccaggaaat aagcctcggt ataagtcacc ccacgctaag
     7441 cgctatgata accgagaaat ggaattttga ctctgaatta accaacgcta ttaaatacca
     7501 tcttcgtcca cacatggcac ctaaaggaat caaggagctt atatatatta tctatttagc
     7561 acatatactt atagaatttg aacacagaaa cattctttat cagtcactgg atttcgatgt
     7621 tttaaagttt tttaacctta aaacagagga agagcttaaa aaactccact caattttaca
     7681 tcaggcttat tcaaaaaaca tgtaactatc tgctcaaata ttccttgacc taacaggctt
     7741 tggttttctt aaatcatttg tacacattac caaataacct aataatcaag gatataaatc
     7801 atgtattttc aggatctaat agcaacgctt aacgagttct ggagcaagca gggctgcatc
     7861 aatatacagg gatacgatac cgaagttgga gcgggaacat ttaatcctgg cactttttta
     7921 cgcgcattag gccccgagcc atggaacgtc tcgtatgttg aaccttcacg acgccccact
     7981 gatggtcgct atggggacaa tccaaaccgc ctacagcact attatcagta tcaagtgata
     8041 atgaaacctt ctcctgaaaa cattcaggat ctttatcttg attctttaaa ggcaataggt
     8101 cttaacctaa acgaacacga cgtccgtttt gttgaagacg attgggaatc tcctactcta
     8161 ggtgcagctg gtcttggatg ggaggtatgg cttgacggta tggaaatcac acaattcacc
     8221 tattttcagc tctgtgggag cctggagctt tctccaat
//
LOCUS       DAOTGZ010000117         8228 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_3064
            NODE_10468_length_8228_cov_5.350300, whole genome shotgun sequence.
ACCESSION   DAOTGZ010000117 DAOTGZ010000000
VERSION     DAOTGZ010000117.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868088
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 8228)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8228)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:22:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,785
            CDSs (total)                      :: 2,751
            Genes (coding)                    :: 2,732
            CDSs (with protein)               :: 2,732
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 12 of 19
            Pseudo Genes (internal stop)      :: 5 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8228
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10468_length_8228_cov_5.350300"
                     /isolate="AMR_MDS_3064"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1122)
                     /gene="aspS"
                     /locus_tag="P5123_08525"
     CDS             complement(<1..1122)
                     /gene="aspS"
                     /locus_tag="P5123_08525"
                     /EC_number="6.1.1.12"
                     /inference="COORDINATES: protein motif:HMM:TIGR00459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016874 - ligase activity [Evidence IEA];
                     GO_process: GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate--tRNA ligase"
                     /protein_id="HRX16344.1"
                     /translation="MFETRKYCGDISSSDIDSEVTLAGWVDRRRDHGGVIFVVLRDVS
                     GLLQVVTDESTSPEQLAVADKLRNEFCIAVKGTVRKRDEANINPNIINGDIEIVAKSI
                     VILNESEVPPFMLDSREHIHEEIRLKHRYLDLRREEMQKTMIARHKIMQATRRFLTDN
                     NFIEIETPTLNKSTPEGARDFLVPSRLHEGHFYALPQSPQLFKQILMVSGFDRYFNIV
                     KCFRDEDLRNDRQPEFTQIDMELSFVNPKMIMNIIEGLLKDIVLAAVERKIETPFPVI
                     SYKEAMERFGKDAPDMRFGFELVDVSDVLKDSEFKVFANNIKNGGIIKALPIDMGDKI
                     SRKMIDDYTEWVKIFRAGGLPNFRYNNGAFEGGIAKFLSD"
     gene            complement(1309..1962)
                     /locus_tag="P5123_08530"
     CDS             complement(1309..1962)
                     /locus_tag="P5123_08530"
                     /inference="COORDINATES: protein motif:HMM:NF012451.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="J domain-containing protein"
                     /protein_id="HRX16345.1"
                     /translation="MDRRLIRYEFNQVVEYLLSQNEPVNESRVIRALAEMHGYPSFGD
                     VSIYTLHFSLYHTLFKIKFSPEYSDYYVHTYTMFIRMLKLPTAGYCCSYNEQNGNFCS
                     NGSLYYYCHKHREDEIWQATPVNAVMSRFYLDPENILFQDSCDLQDRMDKILLYGMCK
                     SRVDYAFYIFGLDSKDYKLIKKRYRQLASKYHPDKGGSIDKMKEINESYALLKKIFS"
     gene            2157..3185
                     /gene="pheS"
                     /locus_tag="P5123_08535"
     CDS             2157..3185
                     /gene="pheS"
                     /locus_tag="P5123_08535"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016560112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009328 - phenylalanine-tRNA ligase
                     complex [Evidence IEA];
                     GO_function: GO:0004826 - phenylalanine-tRNA ligase
                     activity [Evidence IEA];
                     GO_process: GO:0006432 - phenylalanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit alpha"
                     /protein_id="HRX16346.1"
                     /translation="MKEKLELIKHNTLDAISKCASIDELEQIRIDIVGRKGELTSILR
                     SLKDLSPQERGEVGKLSNVIKETINDAIDNRKNDVERALLTNRLSEEWIDVTLDKESE
                     FASNNGYLHPLSQIQHEIEDIFTSMGFAVLDGPEVETEYYNFEALNIPKHHPARDMQD
                     TFWTEDNNLLRTHTSAIQVRGMEKNEPPFRVIGPGRVFRYEATDASHENTFYQVEGMM
                     VDKEISVSNLIFFMKTLLKEIFKKEVKVRLRPGYFPFVEPGFELDINCLICGGNGCSV
                     CKQTGWVELLPCGLVHPNVLKSGGIDPEKWSGFAFGLGLNRLVMMGYGINDIRHFQSG
                     DIRFVQQF"
     gene            3202..5592
                     /gene="pheT"
                     /locus_tag="P5123_08540"
     CDS             3202..5592
                     /gene="pheT"
                     /locus_tag="P5123_08540"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: protein motif:HMM:TIGR00472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009328 - phenylalanine-tRNA ligase
                     complex [Evidence IEA];
                     GO_function: GO:0004826 - phenylalanine-tRNA ligase
                     activity [Evidence IEA];
                     GO_process: GO:0006432 - phenylalanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit beta"
                     /protein_id="HRX16347.1"
                     /translation="MWLSLNILKTMVDLEGLTPEEISANLTMSTAETEGFEEINAHLK
                     TTVTAKLIDVKKHPDSDHLTVVKADTGNAVYNVVCGAPNHKTGDISALALEGTCLGED
                     FIIKKTKIRGEESCGMLCSEKELGLSEESAGIIIFDPKTPIGKPLSEIYPDRMDVRIE
                     IDNKSITHRPDLWGHLGFARELSAIFNRKLINPVNFELKKTFKGNDPLSVSIDCPNAA
                     QRYCGLVVKNIRIEESPEWLKSRIISIGMRPINNIVDITNYVMAELGEPMHAFSRKKL
                     KGDKIIVRMAKTDETLTTLDGGDHKLSSEDIVIADSNNPIAIAGVMGGGNSEIDQDTT
                     EIILEAATFDAVHIRKSAHRHNLRTDAAMRFEKSLDAEICETALIRSYQLIKELIPDA
                     ETNSPFIDAYARKNPELSIDINCDHIRKLLGEPIPDERIIKILTALEYDVVNKTGNLT
                     IGVPSYRATKDIEIAADIVEEVGRVFGYDNITPVAPLVACTTPPKNLFREFERTVKQI
                     LAYDCGLTEVYNYSFVGEDICKKAQINTETQLRLKNPLSIEHDRLRDNMVPAIIQNIA
                     LNSKNHKGFGLFEMGRVYIKDDRQSKDLAKENFRIAGAVYNKSKDIVFYDIKNTILKL
                     VNKLKIKRFEFRIPDKINTYIHPVRSLELFIDGNSAGFIFELSPVVQTDFDFEGRAAI
                     FDIDLDQLYKSQKKSTVFQELQKYPDVNFEISVIADKQEYAINIIRSAKKSNSNFIKA
                     IDVVSVYTGDPLPADKKSVSLKIVFNGNDHTLTSDEIDKLQKKVIESINKDGYHLR"
     gene            5603..5944
                     /locus_tag="P5123_08545"
     CDS             5603..5944
                     /locus_tag="P5123_08545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004622895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine triad nucleotide-binding protein"
                     /protein_id="HRX16348.1"
                     /translation="MMDDLFVKIINGEIPSKKVFENDKVYAFEDINPKAPVHVLVVHK
                     EPTENINDTGLDKAYIYSDLFLAAREIAKKLNIDKTGFRTIINSGPDSGQEVYHMHVH
                     ILGGKKMQNLV"
     gene            complement(5941..7203)
                     /locus_tag="P5123_08550"
     CDS             complement(5941..7203)
                     /locus_tag="P5123_08550"
                     /inference="COORDINATES: protein motif:HMM:NF013741.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin family protein"
                     /protein_id="HRX16349.1"
                     /translation="MLTLIVYQLFILGLFAASAFFSGSETIIISANRYTLEEIKLRDG
                     WGATGALYILDNMEKALSMILIANNIANIGATAFIAYIATKYYKAGESTLLIITVIQT
                     LIFLLFCEIIPKIFSRSNANFLIRLFSGPLVILIIIFSPIVKVIILFTSTLKKVLRIG
                     ESVDARVQARNEIETMFQMGEAAGILEENHRMYVDEIMSLHKIRVSQVMTPTIDIISV
                     EEKQSLRSVIKYIEKTKYSRIPVYRDRVDNITGYIYYKDLYNDYSADTPVFKVMRPAV
                     YVPGTKSIYSLLKEMRQNNKHMIFVINEYGGVEGLTTREDIAEEIVGEIQNTEHISEE
                     IVVKQVNGTYIVKGNIDIDYFCRLLNITIPDKGFETVAGFLNYKLDRIPAVGDQLLYK
                     NHLFTVEEALPTTVTRVRVSPRRRYKRD"
     gene            complement(7197..>8228)
                     /locus_tag="P5123_08555"
     CDS             complement(7197..>8228)
                     /locus_tag="P5123_08555"
                     /inference="COORDINATES: protein motif:HMM:NF013741.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin family protein"
                     /protein_id="HRX16350.1"
                     /translation="IFVTSLTTSILLDKYGRFGHFIAIAIVTPVVIIVCEIIPKIISL
                     SAAVTISKKIINPLNIFHKLFFPIRTVFLIISNILIKLFKLKSDTSMSLSEREIDIAI
                     KLNESRGFIDKDESNFIKNVLRFSKKTAENVMIPRNRAIGVEMNSTIKEAISIFKESG
                     IMRAPVYKNNLDNVVGIIDSRDLIPYIYGQKKGKTIKPLTKKVNFYPESKELVDLLNE
                     FLTNRIQMAVLIDEYGGTSGIVTLSSIVSEVMGESFNLDDSRQRGEVKKVRSATVVDA
                     GMQIDDFNDQFNTDLHSTESETLGGYVLEKLGHLPMRGEFININGYNLIVRNIKRNKI
                     ISLEVKKEC"
BASE COUNT         2696 a         1556 c         1447 g         2529 t
ORIGIN      
        1 atcagaaaga aatttagcga taccaccttc aaacgcgcca ttgttatatc taaagtttgg
       61 taatcctccg gctcggaaaa tcttaaccca ttcagtgtaa tcatcaatca tctttcggga
      121 aattttgtct cccatatcta ttggaagagc ttttatgata ccaccgtttt taatattatt
      181 cgcgaaaacc ttaaattccg aatcttttag tacatcggat acatcaacaa gttcgaaacc
      241 aaaccgcata tccggtgcat cctttccaaa gcgctccatt gcttctttat aactaatcac
      301 tgggaaagga gtctcgattt ttctttcaac ggctgctaaa acgatatctt taagtaatcc
      361 ttcaattata ttcattatca tttttgggtt aacaaatgac aactccatgt ctatttgagt
      421 aaattcaggt tgacggtcat tacgtaaatc ctcgtcccga aagcatttaa caatgttaaa
      481 atatcggtcg aatcctgaaa ccattaatat ctgtttgaaa agctgaggtg actgtggtag
      541 agcataaaaa tgcccttcat gaaggcgtga tggaacaaga aaatcccttg caccctctgg
      601 tgtagattta tttaaagtcg gtgtctctat ttctatgaag ttattgtccg taagaaatct
      661 tcgtgttgct tgcataatct tgtgtcgggc aatcattgtt ttctgcatct cctcccgacg
      721 aagatcaagg taacggtgtt tgagtctgat ctcttcatga atgtgctcac ggctatcaag
      781 catgaacggt ggaacttccg actcgttaag aataactatg ctttttgcaa caatttctat
      841 atcaccgttt attatattag ggttaatgtt tgcttcatca cgtttacgaa cagtaccctt
      901 aaccgctata caaaattcgt ttcgaagttt atcggcaaca gcaagttgct cgggtgaagt
      961 ggattcgtcg gtcacaacct gaagaagacc gctcacgtca cgcaaaacaa caaagatcac
     1021 tccaccatgg tcacgtcgtc tgtctaccca tcctgcaaga gtaacttcag aatcgatgtc
     1081 agaagatgat atatcgccac aatattttct tgtttcaaac attattttcc tcttttagaa
     1141 gctcaaattg agcagattac tactctattc ccgattgtgc tcttttttgt caatacataa
     1201 ttatctgctt tttttatgat tcagctccat taaaaattgc aaaaattttt aaaatattac
     1261 cattttgaat gactatacgt ttctaagccg atatatgaaa aacatttgtt aagaaaaaat
     1321 tttttttaac agcgcgtatg attcattgat ctctttcatc ttgtcaatgc taccaccttt
     1381 atcgggatga tactttgaag ctagctgacg atacctcttt tttattaatt tataatcttt
     1441 agaatcaaga ccaaaaatat aaaaagcgta gtcaaccctg gatttgcaca taccgtataa
     1501 tagaatcttg tccatgcgat cttgcaaatc gcaactatcc tgaaacaaga tgttttcagg
     1561 atcaagatag aagcgggaca taacagcatt tacaggagta gcctgccaaa tttcatcttc
     1621 acgatgttta tgacaataat agtatagaga tccgttactg caaaaatttc cattttgctc
     1681 gttatacgaa cagcaatatc cagcggttgg caacttaagc attcttataa acatggtata
     1741 ggtatggaca taataatcgc tgtattccgg tgaaaatttg attttgaata gtgtatggta
     1801 aagtgaaaaa tgtaaagtat agatagagac atcaccgaat gatggataac cgtgcatttc
     1861 ggcaagagca cgaatcaccc tgctttcatt cacaggttca ttttgagaaa gcagatattc
     1921 aacgacttga ttaaattcat atctaattaa acgtctatcc atttctacaa catagaaatt
     1981 aaacatcgag gtgtcaacaa aagcttaccc ctcttgcgac atccctatgt tcaataattt
     2041 tcttgacttt taacatacac gtaaaatgaa agagatagct ttatttttca tttataagca
     2101 aatttaacaa tatttttata aaccacgatt agttttactc tggttgggac aatctcatga
     2161 aagaaaaact cgaactgatt aaacacaaca ctctcgacgc cattagcaaa tgtgcatcca
     2221 tagatgaact ggaacagata cgaatagata ttgttggccg aaaaggtgaa cttacatcca
     2281 ttttacgttc tctgaaagac ctttcgcccc aggaacgtgg agaagtcggt aaactgtcaa
     2341 atgtgattaa agagacaatt aacgatgcga ttgacaatcg taaaaacgat gttgagcgtg
     2401 ctttacttac aaaccgttta tctgaagagt ggatagatgt aactctggat aaggaatccg
     2461 aatttgcgtc aaataatgga taccttcacc ctctttcaca aatacaacat gaaattgaag
     2521 atatttttac atcgatgggc tttgcggttc ttgacggtcc tgaggttgaa acagaatatt
     2581 ataactttga agctctcaac ataccgaaac atcatccagc tcgcgacatg caggacacct
     2641 tctggacaga agacaataac cttcttagaa cccatacctc agccatacag gttcgcggca
     2701 tggaaaaaaa cgaaccaccg tttcgtgtta ttggcccagg tcgtgttttt agatacgaag
     2761 cgacagatgc ttcacacgaa aatacctttt atcaggtcga aggcatgatg gtagataaag
     2821 agatctctgt ttccaatctt atttttttca tgaaaacact tttaaaggaa attttcaaaa
     2881 aagaggttaa agtcagacta cgacccggat attttccgtt tgtcgaacct ggtttcgaac
     2941 ttgatataaa ctgtctgata tgtgggggta acggatgttc tgtttgtaaa cagacaggat
     3001 gggtagaact gctaccttgc ggacttgttc accctaatgt tttaaaatcg ggtggaattg
     3061 atcctgaaaa gtggtctggt tttgcttttg gtctgggatt aaacagactt gttatgatgg
     3121 gttatggaat aaatgacata cggcattttc aaagcggcga tattcgcttt gtacaacaat
     3181 tttaggtaaa ggggacaaac tatgtggctg tcacttaata tattaaaaac tatggtcgat
     3241 cttgagggac tcaccccgga agagatctcg gcaaatttga ccatgtctac tgcagaaacc
     3301 gaaggcttcg aagagattaa cgcgcatcta aaaactaccg ttactgcaaa actcattgat
     3361 gtaaaaaaac atcccgattc ggatcacctt accgtagtta aagctgatac cggaaatgct
     3421 gtttacaatg tagtatgcgg cgcacctaat cacaaaactg gcgatatttc tgctcttgcc
     3481 cttgaaggca cctgcttagg tgaagatttt attatcaaaa agacgaaaat acgcggagag
     3541 gaatcatgtg gcatgctctg ctctgaaaag gaacttggac tatctgagga gtctgccggc
     3601 attattattt ttgatccgaa aacacctata ggaaaacctc taagtgaaat ttaccctgac
     3661 agaatggatg taagaatcga gattgataat aaatcaatta ctcaccggcc tgatctttgg
     3721 gggcacttgg gttttgcacg ggaactttct gcaattttca accgcaagct tatcaatcct
     3781 gtaaatttcg aattgaagaa aacatttaaa ggaaacgatc ctctttcagt ttccatagat
     3841 tgtccaaatg ccgcgcaacg ttattgtgga ctcgtcgtaa aaaatattcg cattgaagag
     3901 tcgcctgaat ggttaaaatc caggataatt tcaatcggca tgagacctat taataatatt
     3961 gttgatatta ccaactatgt tatggccgag cttggtgagc caatgcacgc tttcagccgt
     4021 aaaaagctaa aaggggacaa gataatcgtt agaatggcca aaaccgatga aaccctaaca
     4081 acactagacg gtggagatca taaactttca tcagaagata ttgttatagc cgattcaaac
     4141 aatccgatcg ctatcgcagg tgtaatgggc ggtggcaact cggaaattga tcaggacaca
     4201 acagaaataa ttttggaggc agcgacattt gatgctgtac atatccgcaa gagcgctcat
     4261 cggcacaatc ttagaacaga tgccgctatg agatttgaaa aatctcttga tgccgaaata
     4321 tgcgagactg ccttaatacg gagctatcag cttatcaaag aacttatccc cgatgccgaa
     4381 acaaattctc cttttattga tgcttatgct cgtaaaaacc ctgagttaag tatagatatc
     4441 aattgtgacc atatacggaa acttcttgga gaaccaatac ctgatgaaag aataataaaa
     4501 attcttaccg ctcttgaata cgatgttgtt aacaaaaccg gtaacttgac tattggtgtt
     4561 ccttcttacc gtgccactaa ggatatcgaa atagcagccg atatagttga agaggtaggt
     4621 cgtgtttttg gatacgacaa tatcacaccg gtcgcacctc ttgtagcatg taccacacca
     4681 cctaaaaacc ttttcagaga atttgaaaga acagtaaaac agatattagc ttacgactgt
     4741 ggtttaacag aagtgtataa ctactcattt gtaggagagg atatctgtaa aaaagcccag
     4801 attaataccg agactcaact aaggttaaaa aacccattat caattgaaca cgacagactt
     4861 cgagacaata tggtaccagc aattattcaa aatattgctc ttaacagcaa gaatcacaaa
     4921 ggtttcggcc ttttcgagat gggacgagtc tatataaaag acgacagaca atcaaaggat
     4981 ctagcaaaag aaaatttcag aattgctgga gccgtataca ataagtcaaa agatatcgtc
     5041 ttttatgata taaaaaatac aatactaaag cttgttaata aacttaaaat caaaagattc
     5101 gaattcagaa tcccagataa aataaataca tacattcatc ctgtacgatc actagagcta
     5161 ttcatcgacg gcaattcagc cggatttatt tttgaactta gccctgttgt tcaaactgac
     5221 tttgattttg agggaagagc agccatattt gatatcgatc ttgatcaatt atataaatca
     5281 caaaaaaaga gcacagtatt tcaagaacta caaaaatatc ctgatgtcaa tttcgaaata
     5341 tccgtgattg ccgataaaca agaatatgct ataaacatca tacgatctgc taagaaaagc
     5401 aattcaaatt tcattaaagc aatagatgtg gtatccgttt acactgggga tcccttacct
     5461 gctgataaaa aatctgtttc attaaaaatt gtttttaacg gaaatgatca tacactaacc
     5521 tctgatgaaa ttgacaaatt gcaaaagaag gtaattgaat caatcaataa agatggttac
     5581 cacttaagat aagaaggagg agattatgga cgacttgttt gttaaaataa taaacggaga
     5641 aattccttca aagaaagtat ttgaaaacga taaggtttac gctttcgaag atattaatcc
     5701 aaaagcccca gttcacgttt tagtcgttca taaagaacct accgagaaca ttaatgatac
     5761 cggtttagac aaagcttata tctattcgga tcttttttta gctgcaagag aaattgcaaa
     5821 aaagttaaac attgataaaa cagggtttcg tacaataatt aattcagggc cggactcagg
     5881 gcaggaagtt tatcacatgc atgtgcatat tcttggtggt aaaaaaatgc aaaatcttgt
     5941 ttaatctcgc ttgtatcgtc ttctaggcga tactcgcaca cgagtaactg ttgttggtaa
     6001 agcttcctcc acagtgaaga gatggttttt gtagagtaac tgatctccaa cagcaggaat
     6061 tcgatccagc ttgtagttta gaaaaccagc tacagtttcg aagcccttat ccggtatggt
     6121 tatatttaac agtctgcaga aatagtctat gtctatattg cctttaacaa tatatgtacc
     6181 gttaacctgt tttactacta tctcttccga aatatgctct gtattttgaa tttcacctac
     6241 aatttcctca gcaatgtctt cacgtgttgt tagaccttct acaccaccat attcgttaat
     6301 tacaaaaatc atatgtttgt tattttgacg catctctttc aaaagtgaat atatagattt
     6361 cgtaccaggt acatatactg cgggtcgcat aactttgaac acgggtgtat cagcagaata
     6421 atcgttatat aaatctttat aatagatata tccggtaata ttatcaacac gatcccgata
     6481 aacaggaata cgtgaatatt ttgttttttc gatatatttt ataacagaac gcaaactctg
     6541 tttctcctca acagaaataa tgtcaattgt tggggtcata acctgactta ctctaatctt
     6601 atgtagcgac attatttcgt caacatacat tctatggttc tcttcaagta tgccagcagc
     6661 ctctcccatc tgaaacattg tctcgatctc gtttcgagcc tgcactcgcg catccacact
     6721 ttcaccaatt cttaaaactt ttttcaaggt agaggtaaaa agaataatga cctttactat
     6781 tggagaaaaa ataataataa gaataaccag aggaccagaa aaaaggcgaa ttaaaaaatt
     6841 cgcattcgag cgcgagaata ttttgggtat aatttcacaa aataaaagaa aaattaaggt
     6901 ttgtataacc gtaataatca atagagttga ttcgccagct ttataatact ttgtagcgat
     6961 ataggctata aacgcggtgg ctccaatgtt tgcaatatta ttggcaatta aaatcattga
     7021 aagagctttt tccatattat ccaatatata aagtgctccg gtagctcccc atccgtcgcg
     7081 taattttatc tcttctaggg tataacggtt cgcagatata attatggttt ctgatcccga
     7141 aaaaaatgct gatgctgcaa atagccccag aataaagagt tgatatacaa ttaaggtcag
     7201 cattcttttt tgacttccaa agagattatt ttgtttcttt taatatttct gactatcaga
     7261 ttgtatccat tgatgtttat aaattcgcct cgcatcggta aatgtcccag tttttccaga
     7321 acatagcctc ccaaggtctc agactcagta ctgtgtaaat cagtattaaa ctgatcattg
     7381 aaatcatcaa tctgcatacc agcatccaca acagttgccg atctaacttt ttttacttca
     7441 cccctctgtc ttgaatcatc aagattaaaa ctttcgccca ttacctcact aacaatagat
     7501 gaaagagtaa caataccgga agtccctccg tactcatcaa ttagaaccgc catctgaata
     7561 cgattggtca aaaactcatt tagaagatcg acaagctctt ttgattcagg ataaaagttt
     7621 accttttttg taagaggttt tatggttttt cctttttttt gaccatatat gtatggaatt
     7681 aaatctcttg aatcgataat tcccacaaca ttatcaagat tatttttata aacaggtgct
     7741 cgcataattc cggattcttt aaatatagaa atagcctctt tgattgtcga attcatttcg
     7801 acaccaattg ctctgttacg tggaatcatt acgttttcag ctgttttctt tgagaaacgc
     7861 agaacatttt ttataaaatt gctctcatcc ttgtcgataa aacctcttga ttcatttagt
     7921 tttattgcaa tatctatttc acgttctgag agactcatgg aagtatctga ttttagttta
     7981 aagagtttta ttaatatatt agaaattatt aaaaaaacag ttctaatcgg gaaaaatagt
     8041 ttatgaaaaa tatttagagg atttattatt ttttttgaaa ttgtaactgc cgctgacaaa
     8101 gatattattt taggaataat ttcacacaca ataataacaa ccggtgttac aattgctatt
     8161 gctataaagt gaccgaacct tccatactta tccagaagaa ttgaagttgt aagagaagta
     8221 acaaagat
//
LOCUS       DAOTGZ010000118         8204 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_3064
            NODE_10515_length_8204_cov_4.458952, whole genome shotgun sequence.
ACCESSION   DAOTGZ010000118 DAOTGZ010000000
VERSION     DAOTGZ010000118.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868088
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 8204)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8204)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:22:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,785
            CDSs (total)                      :: 2,751
            Genes (coding)                    :: 2,732
            CDSs (with protein)               :: 2,732
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 12 of 19
            Pseudo Genes (internal stop)      :: 5 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8204
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10515_length_8204_cov_4.458952"
                     /isolate="AMR_MDS_3064"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1881
                     /gene="thrS"
                     /locus_tag="P5123_08560"
     CDS             <1..1881
                     /gene="thrS"
                     /locus_tag="P5123_08560"
                     /EC_number="6.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019642483.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004829 - threonine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006435 - threonyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="threonine--tRNA ligase"
                     /protein_id="HRX16351.1"
                     /translation="DGSLLEAAKGTTVYDLIGKIGAGLQKAAVAAAIDGELVDLTYVV
                     EKDASLTVHTFKDDTGQSIYWHSTSHILAQAVKRLYPDAKYAIGPSIDNGFYYDFDCK
                     TSFTPDDLEKIEKEIEKILAEALSVKRTVLSCKDARSKFEKMGETYKVELIDDLETDT
                     VTVYEQGEFSDLCRGPHIINTSVIGAVKLTKVAGAYWRGNEKNQMLQRIYGVSFPDKK
                     QLKKHLDLLEEAKERDHRKIGKEMSLFEFSQDVGPGLTMWTPKGARIRTVIEDFWKKQ
                     HYRNGYDIVYTPHIGRSRLWEISGHLDFYKDGMYSAMDIDGEDYYVKPMNCPFHIILY
                     KSEKRSYRDLPLRWAEFGTVYRYEKSGTLHGLLRVRGFTQDDAHLFIREDQLTEEISR
                     TFKFMIFMLKSFGFEQFQVYLSTRPEKAVGSVEIWQKSEAALAESLKHLGVEYSLNEG
                     DGAFYGPKIDVKVVDAIGRQWQLSTLQLDFNLPERFDMTYTGADGAEHRPIMIHRALL
                     GSLERFFGILIEHYKGAFPAWLAPVQVMLLNVGEDVIDDVKRVRDNLRSEGIRVEMDL
                     RNETMGYKIREAVTSKAPYVCVIGHKEVEEGSVSVRTRGSQKGVSMNIEDLIDDIKKQ
                     AV"
     gene            complement(1908..2819)
                     /locus_tag="P5123_08565"
     CDS             complement(1908..2819)
                     /locus_tag="P5123_08565"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRX16352.1"
                     /translation="MISDLTEKVIYLLTEKKGIPINEMKTTLSRLTPEEFNTIVITLL
                     DKDGSELFELFNYYLILFKDIDKIKELLGSPLISIDVLEKILLISYTRAKIQGIEEDQ
                     ALDNILIFINQSRYLELLLHSDIISKDRMLSYYLLTKLDRQGIDTFFNSNPSIKDFLN
                     GFIRLPDRTIKAILLRNPDLFGYISLFLKTMENEKAESFVKQFRVEIEEMEATKRIVL
                     DLSERFNIHNEKKLPLSERNKERLSEIIRKVKSMNNIFNTVYTLEHEGVIIDSVERTL
                     IMEIMQNPVFKDILEKFSARQTEELSA"
     gene            3158..4009
                     /locus_tag="P5123_08570"
     CDS             3158..4009
                     /locus_tag="P5123_08570"
                     /inference="COORDINATES: protein motif:HMM:NF018076.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L11 methyltransferase"
                     /protein_id="HRX16353.1"
                     /translation="MFQLKIVIKNEQTEEYESALFEAGASSVSISEAGDDNIAIIALF
                     FESDAAYRILDKLNIVGTVTPLLSDYSDRWLDYIDVEDIGDGLTVCKIDENSPSAEVL
                     EKKYGMGGTRCVIGIDARGTFGIGVHPTSRICVGMIREVLKYVYPQESSCADIGSGSG
                     VLSIYAAKLGFGEVVACDISADACLMTEKNSLYNRCRVKVVNCGIKQFNAGPFDVVVA
                     NIPISLTEISINELESRVRIGGYLLLSGFGLKWKDYVSSAFSKAFTAVFFTTSGDWGG
                     YIMKKEQ"
     gene            4182..4730
                     /gene="infC"
                     /locus_tag="P5123_08575"
     CDS             4182..4730
                     /gene="infC"
                     /locus_tag="P5123_08575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013623487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003743 - translation initiation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006413 - translational initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-3"
                     /protein_id="HRX16354.1"
                     /translation="MALNNRKPRSKTASGKEYRINDMIKAESVRLVGDGDEPVIVTLE
                     KALELAEQSELDLVEISMQDVPVVRIMDYSKFKFEQVKKAKEAKKKQKVVHLKEVKVR
                     PSIDAHDYEHKVNHAREFLEKGDKVKVTMMFRGREIIHSDLAMKKMQQMLGELSDCST
                     VEKTPSIEGRNMTMILTPISRN"
     gene            4763..4960
                     /gene="rpmI"
                     /locus_tag="P5123_08580"
     CDS             4763..4960
                     /gene="rpmI"
                     /locus_tag="P5123_08580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004696338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L35"
                     /protein_id="HRX16355.1"
                     /translation="MPKMKSNSGAKKRFKFTSSGKIKRAKAFANHMLECKSPQRKRNL
                     RGTTVSAEADQKRIRRMLPYA"
     gene            5073..5420
                     /gene="rplT"
                     /locus_tag="P5123_08585"
     CDS             5073..5420
                     /gene="rplT"
                     /locus_tag="P5123_08585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011133289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L20"
                     /protein_id="HRX16356.1"
                     /translation="MPRAKSGTIHKKKQRKILKAVKGFRGARSVLFRTAKDARRRALT
                     NSYAHRRARKRDMRALWIVRINAAARLCGTTYSRLINDMKNAGVILNRKVLADLAVYD
                     LKAFESLVKQLNG"
     gene            5413..6294
                     /locus_tag="P5123_08590"
     CDS             5413..6294
                     /locus_tag="P5123_08590"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRX16357.1"
                     /translation="MADFKKKLNYIKGTVLAVLFLSWPALYGKPLCPQTHGMQLGDFQ
                     LSPFVTIHSDGGFYVGSLIAASLSDRTVLKVTHPLYQQTKGGLSGDVCLGLDHFVGGS
                     YGFLFYVSESLNLPFSESVYDNSGDFTHDYGIYTLQTSFKMIYAFPGFSVYGEFSHVF
                     MSHNQGDLFEPLRADIFNKKTWTSMFGLSPFYADGFTSSESRAFDWYGVEVGPRTAVG
                     MLRMEVMFYYSAPYSEKKSLEDIPLEGFLPLMMVCPEVEWFYSETFSISIGAGYKLLG
                     VSGYPRYRAYSSASVIF"
     gene            6673..7632
                     /locus_tag="P5123_08595"
     CDS             6673..7632
                     /locus_tag="P5123_08595"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRX16358.1"
                     /translation="MVSMKQLEELESRVVKALQLISDLRAENSKLESDNDVLKADAEE
                     VKLSLEEKEQQIAQIQKELDASTRELASLRDKEDQLEQRIIALLEKMDMLQAGGSIPA
                     GDSRPVSKPSAAVAAASAPVLEPEEEEDFEEPIVDDVEDEDIDDITIEDESEDLIIED
                     EGEDLIIDDSDDSDDDFGSIDLDEDDDLVLDDDTEDLDLGDEEIIMDDDLELDEDESS
                     DDDDIVIIDDEGEDVEAFSGDDDDESGDEFSIDEEDETDDSELDDSDDEEEDEDDIIL
                     LDDEDDDEIILDEDDDEIIIDESDEEDFDDDDDDFIIVEEDDK"
     gene            7659..7973
                     /locus_tag="P5123_08600"
     CDS             7659..7973
                     /locus_tag="P5123_08600"
                     /inference="COORDINATES: protein motif:HMM:NF017016.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000917 - division septum assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZapA"
                     /protein_id="HRX16359.1"
                     /translation="MKEISKVNVTIFGSTYNIQGDAPIEYISSLARYVDKKMDEVSSN
                     MKSPNLLQVAVLAALNIADEYHQLKRLDNNQSDDITKRASALISMLEEGLIGDILDKP
                     KN"
     gene            7989..>8204
                     /locus_tag="P5123_08605"
     CDS             7989..>8204
                     /locus_tag="P5123_08605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012470855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis protein CheW"
                     /protein_id="HRX16360.1"
                     /translation="MEISELEKQQESLVLQMGKVGDGTVKTDDGEEVSEGQIVQLVSF
                     TLDDVSYGVDILSVHEILRIPQITRLPN"
BASE COUNT         2472 a         1326 c         1920 g         2486 t
ORIGIN      
        1 gatggttcgt tactggaggc tgccaaggga actacggtat acgaccttat tgggaaaatc
       61 ggtgccggtc ttcaaaaagc agctgttgct gcagctattg atggtgaact agttgattta
      121 acttatgtag tagagaagga tgcgtcttta actgttcata cctttaaaga tgatactgga
      181 caatcaattt actggcactc gacttcgcat attcttgcgc aagccgtcaa gcgactttat
      241 cccgacgcaa agtatgcaat agggccatca atcgataatg gcttctacta cgattttgac
      301 tgtaaaacaa gttttactcc agatgacctt gaaaaaatag aaaaagagat agagaagatt
      361 ttggcagagg ctctttctgt taagcgtaca gtgcttagct gtaaagatgc ccgttcaaag
      421 ttcgagaaaa tgggtgagac ctacaaggtc gaactgattg atgatcttga gactgataca
      481 gtaactgttt acgagcaagg tgaattttct gatctttgtc gtggccctca cataatcaat
      541 acatctgtaa ttggtgctgt caagttgacc aaagttgccg gtgcttactg gaggggcaac
      601 gaaaaaaatc agatgcttca gcgtatctat ggggtttcgt tcccagataa gaagcagctt
      661 aaaaaacatc ttgatctgct tgaagaggcc aaagagagag atcaccgcaa aatcggaaaa
      721 gagatgtctc ttttcgagtt ttctcaagac gtaggtcctg ggcttacaat gtggacacct
      781 aaaggtgcca gaataagaac agttattgaa gacttctgga aaaaacagca ttaccgtaac
      841 ggttatgata tagtctatac tccgcatatt ggacgttcac gtctttggga aatttccgga
      901 caccttgact tttacaaaga cggaatgtat tctgcgatgg atattgacgg tgaagattac
      961 tatgtaaaac cgatgaattg ccctttccac attatactct ataaatctga aaagcgcagt
     1021 tatcgtgatc ttccacttcg ctgggccgag tttggtactg tttatcgcta tgaaaaatcg
     1081 ggaactcttc acggtctgtt gcgtgttcgt gggtttacgc aagatgatgc acatcttttt
     1141 attcgtgaag atcagcttac cgaagagatt agccgcacat tcaagtttat gatatttatg
     1201 ctcaagagtt ttggatttga gcaatttcag gtttatttat caacccgtcc ggaaaaggca
     1261 gtcggttctg ttgagatatg gcaaaagtcc gaagcggcgc ttgccgaatc gcttaaacat
     1321 cttggtgtcg agtacagctt aaacgagggt gatggagcct tttacggtcc caaaatagat
     1381 gtcaaggttg ttgacgcgat tggccgccaa tggcagctct ctacactgca acttgatttc
     1441 aatttgcccg aaagatttga catgacatat accggagccg acggtgctga gcatcgtcca
     1501 ataatgattc accgggctct tttggggtcg cttgagcgat tttttggtat tcttattgag
     1561 cattacaaag gagcatttcc agcttggctt gcgcctgttc aggttatgct tttaaatgtt
     1621 ggcgaagatg ttattgatga cgttaagcgc gtgcgtgata atcttcgttc agaaggtata
     1681 cgtgttgaaa tggatcttcg aaatgaaact atgggttaca agatacgaga agcggtaacc
     1741 tctaaagccc cctatgtctg tgttataggt cacaaagagg ttgaagaggg aagtgtttct
     1801 gtaaggaccc gcggttcgca gaaaggtgta tcgatgaata tagaagatct tattgatgat
     1861 ataaagaaac aggcggttta accgtctgtt tcggacgcta gtcggtatca ggcagaaagt
     1921 tcttctgttt ggcgggcgct gaatttttcc agaatatcct taaataccgg gttttgcatt
     1981 atttccatta tgagtgttct ctctacgctg tcaatgatga ctccttcgtg ctctaaagtg
     2041 taaacggtat taaagatgtt gttcatggat tttacttttc taattatttc agatagtctt
     2101 tccttgtttc tttcggagag tggcaacttc ttttcgttgt gtatattgaa gcgttcactt
     2161 aaatcaagaa caatcctttt tgtcgcctcc atctcttcta tttcgactct aaactgtttt
     2221 acaaaacttt cagccttttc gttttccatg gttttaagaa aaagcgaaat atagccgaaa
     2281 agatcggggt tgcgtaaaag gatagcctta atggttctat cgggtaaccg gataaaaccg
     2341 tttaaaaaat ctttaattga agggttcgag ttaaaaaaag tatcaatacc ctgtctatca
     2401 agttttgtaa gcaaatagta agaaagcatg cgatccttgg aaattatgtc cgagtgtaac
     2461 aagagttcaa gatatctgct ttgattaata aaaataagaa tgttatctag agcttggtct
     2521 tcttcaattc cctgaatctt ggctcgagta tatgagataa gaagtatttt ttccaaaacg
     2581 tcaattgata tcagaggact tccaagaagt tcctttattt tgtctatatc tttaaagagt
     2641 atcagataat agttaaaaag ttcaaaaagt tcacttccgt ctttatcaag aagtgtgatt
     2701 acgatagtat taaattcttc cggtgttaac cgggataggg tggttttcat ctcgttgatg
     2761 ggaatgcctt ttttttctgt taaaaggtag attacctttt ctgtaagatc gcttatcatt
     2821 ggggttaagc ctttttttca ttatataatt ttagtctata ttagcggggg tgagcgatta
     2881 gtcaatcata tagttgcagg ttaacactta attcggagtt ggataccttc ggtctgattt
     2941 gcttgaagag agcttgtgaa atgcagatat agtttagagt attttaatat tgactttaat
     3001 taaaatgaat ttgagaataa aatctgattt aggggagctt atatgtcaaa aaagatatct
     3061 ttacgtattg atgattctgt aaaaataatc tctatgatcg cattagagcc aagagatagg
     3121 gcatatcgta attagcttat actgaggttt taaagcgatg ttccagttaa aaattgtgat
     3181 aaaaaatgag caaaccgaag agtatgaatc tgcgcttttt gaagcgggtg catcttcagt
     3241 atcaattagt gaagcagggg atgataatat tgcaattatt gcactttttt ttgaaagtga
     3301 tgctgcttac agaatactgg ataaattgaa tattgttggc acggttactc ccctcttgtc
     3361 cgactacagt gatcgatggc ttgattatat tgatgtcgaa gacattggtg acggtttaac
     3421 tgtttgcaaa atagatgaga attcaccttc ggccgaagta ttagaaaaaa agtacggtat
     3481 ggggggtaca cgttgtgtta tcggtataga tgctcgagga actttcggta ttggtgttca
     3541 cccaactagc cgaatatgcg tgggaatgat cagggaggtc cttaaatatg tctatccaca
     3601 agagtcaagc tgtgccgata tcggaagcgg tagcggagtg ctttcaatat atgcagcaaa
     3661 attggggttt ggtgaggttg tggcgtgcga tatttcggct gatgcctgcc ttatgacaga
     3721 aaaaaacagc ttatataatc gctgtcgggt gaaagttgta aattgcggca ttaaacaatt
     3781 taatgctggt ccttttgacg tagtcgttgc caatataccg atctcgctta cagaaatttc
     3841 tattaatgaa cttgaaagcc gggttagaat cggggggtat cttttgctga gtggttttgg
     3901 tctaaaatgg aaagattatg tctcttcagc cttttcaaag gcgtttacag ctgttttttt
     3961 taccaccagt ggtgattggg gcggatatat aatgaaaaaa gagcagtaat aagtcaaaac
     4021 taaaaaaata tagttgataa atatcccccc attatgaaat tctgacagaa tatttgcgga
     4081 taatgtctgt gaatgttgaa ctgtcgcagt ctgatgggtt gcggtaagat aatattaaca
     4141 ggaagcatac aggcaacctg gaagcttcat aggaggaagt catagcttta aataaccgaa
     4201 aaccccgtag caaaacagca tcgggtaaag agtatcgtat aaacgatatg ataaaagcgg
     4261 agtctgtcag gctagttggc gacggtgatg agccggtaat cgttacactt gaaaaagctc
     4321 tagagcttgc cgaacagagt gaacttgatc ttgtagaaat atccatgcag gatgtgccag
     4381 ttgtcagaat catggattat agcaagttta agtttgaaca ggttaaaaag gcgaaagagg
     4441 caaaaaagaa gcagaaggtt gttcacctta aggaagttaa ggttcgtcct tcgattgatg
     4501 cccatgacta cgagcataag gtgaatcacg cacgtgaatt tctggaaaaa ggtgataagg
     4561 taaaggttac catgatgttc cgggggcgtg aaattattca cagtgatctt gctatgaaga
     4621 aaatgcagca gatgcttggt gagctttcag attgttcgac agtcgaaaaa acaccttcta
     4681 tcgaaggccg taatatgacg atgattttga ctccgatatc tcgtaattaa taattaagca
     4741 tatcaagtaa aggatatata aaatgccaaa aatgaaaagc aacagcggag cgaaaaaacg
     4801 tttcaagttt accagctccg gaaagatcaa gcgtgcaaag gcttttgcca accacatgct
     4861 tgagtgtaag tctccgcaac gcaagcggaa cttgagaggt actacggtat ctgcggaagc
     4921 tgatcagaaa agaatcagaa gaatgcttcc ctatgcctga tttatgcgtc cgttttgggg
     4981 tgcaaaaagc agaagtgaaa cgcggataga agcaggttct gccgtaacct ccccgtttaa
     5041 ctctgtagct taatgtaaaa gaggatttaa caatgccaag agcaaaaagt ggaacaatcc
     5101 acaaaaaaaa gcagcgaaaa atattaaagg cggttaaggg attccgagga gctcgttccg
     5161 ttctttttag aacagcaaag gatgcccgta gaagagcgct tactaactca tatgcacaca
     5221 gacgtgcgcg taagagagat atgagagctc tttggatcgt tcgaatcaat gcggccgcca
     5281 gattatgcgg aacaacatac agccgtctta ttaatgacat gaaaaatgct ggtgtgattt
     5341 taaaccgaaa ggttcttgct gatctcgctg tgtacgattt aaaagcgttt gagtcacttg
     5401 taaagcagct caatggctga ttttaaaaaa aaattgaatt atataaaagg caccgttttg
     5461 gcggtgcttt ttttgtcatg gcctgcactc tatggtaaac cactttgtcc tcaaactcat
     5521 ggtatgcaat taggtgattt tcagctttct ccatttgtta caatccattc cgatggtggt
     5581 ttttatgttg gttcattaat agccgcctct ttaagtgacc gaacagtttt gaaggtaaca
     5641 caccctttgt atcagcagac aaaagggggg ctttctggtg atgtctgcct agggttggat
     5701 cacttcgtag gcggttcgta tggctttctg ttttatgttt cggaaagttt aaacttaccg
     5761 ttttctgaaa gcgtatatga taatagtggt gattttactc atgattatgg tatatatacc
     5821 cttcagacct cttttaaaat gatttacgct ttccctggtt tttcggttta tggagagttt
     5881 tctcatgttt ttatgtcgca taatcaggga gacctttttg aacctctccg agcagatatt
     5941 tttaataaga aaacatggac ttcaatgttt gggctttctc cgttttacgc tgacggtttt
     6001 acctcttcgg aaagtcgagc ctttgattgg tatggagtag aagtagggcc acgaacagca
     6061 gtcggaatgc tgaggatgga agttatgttt tactattcgg ctccctattc agaaaaaaag
     6121 agtctggaag atattccatt ggaaggtttc ttgccattga tgatggtttg cccagaggta
     6181 gaatggtttt acagtgaaac attttcaatt tcaattggag cgggatataa actcttggga
     6241 gtatccggct atccccgtta cagagcctat agttctgcca gtgtaatttt ctaaaagggt
     6301 tttttgtcga actttatcgc aaaaaaagga atcttttccg gttttgtgtt aactgtgctg
     6361 tttttaagtt gcggttttta taaaatgaac ctattcatgt tataataatg gatgtatcta
     6421 tatactaaaa aaataaaatt gtacaagaat caggtttttg cttgttatta ctttattggt
     6481 ggagtaatat ttaatgtaga cgctattcgc tgattaatga aacataaagt acttgtttta
     6541 attgaaaaaa agatgaaatg tgccgattat atgtgtagga ttaatatgtt ttctattctt
     6601 aaacttgaag agaattagaa taaacagaaa aaagatgata taataacaaa tgggtcatat
     6661 gggaggtatt cgatggtatc aatgaagcaa cttgaggagc tggaaagcag ggtagtaaag
     6721 gcgctgcagc ttataagtga tttacgggct gaaaattcga agcttgagag tgataatgat
     6781 gtattaaaag ctgatgccga agaggtaaag ctctctcttg aggaaaaaga gcaacaaata
     6841 gcccagatac aaaaagaact ggatgcttct actcgggaat tggcttcgtt gagggataaa
     6901 gaggatcagc ttgaacagcg tattattgct cttttagaga agatggatat gcttcaggct
     6961 ggtggttcca ttccggctgg tgattcgcgt ccagtatcga aaccttctgc agcagtggct
     7021 gctgcttccg ctcctgttct cgaaccagaa gaggaagagg atttcgaaga gccgattgta
     7081 gatgatgtcg aggatgaaga tattgacgat atcactatag aggatgaatc agaagatctg
     7141 ataattgaag acgaaggtga agatcttata attgatgact cggatgattc tgatgatgat
     7201 tttgggtcta ttgatttgga tgaggatgat gatcttgtgc tggatgatga tactgaagat
     7261 cttgacctcg gtgatgaaga gataatcatg gatgacgatt tggagcttga tgaagacgaa
     7321 tcttcggatg acgatgatat agtaattatt gatgacgaag gtgaagatgt ggaagcattt
     7381 tcgggtgacg atgatgatga gagcggagat gagttctcta tagatgagga agatgaaact
     7441 gatgattcgg aactcgatga ttctgatgac gaagaagagg atgaagatga tattattctt
     7501 ctggacgacg aagatgatga cgagattata ctggatgaag atgatgatga gataatcatt
     7561 gacgaatcag atgaagaaga ttttgatgat gacgatgacg attttatcat tgtggaagag
     7621 gatgataaat aaaatatctg ttatccaggt aataaaacat gaaagaaata tcaaaagtta
     7681 atgtaacgat atttggaagt acctataata ttcagggaga tgcgcctatt gaatatatat
     7741 cgtctttggc tcggtatgtt gacaaaaaaa tggatgaggt cagttctaat atgaaatcgc
     7801 cgaatttgct ccaggtagca gttttggcag ctttaaatat tgctgacgag tatcatcagc
     7861 tcaaaagact ggataataat cagagtgatg atataaccaa aagagcgtcc gcgcttatat
     7921 cgatgcttga agagggtctt atcggggata ttctggataa acctaaaaat taataacagg
     7981 gaaaactgat ggaaattagt gaattggaaa aacagcagga atcattggtt ctgcagatgg
     8041 gtaaggtcgg agacggaact gtcaagaccg atgatggtga agaagtttca gaaggacaaa
     8101 ttgtacagct tgtaagtttt acacttgatg atgtttctta tggtgtggac atactatctg
     8161 ttcatgagat acttcgtata ccgcaaatta cccgtcttcc aaac
//
LOCUS       DAOTGZ010000119         8154 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_3064
            NODE_10603_length_8154_cov_4.213360, whole genome shotgun sequence.
ACCESSION   DAOTGZ010000119 DAOTGZ010000000
VERSION     DAOTGZ010000119.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868088
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 8154)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8154)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:22:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,785
            CDSs (total)                      :: 2,751
            Genes (coding)                    :: 2,732
            CDSs (with protein)               :: 2,732
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 12 of 19
            Pseudo Genes (internal stop)      :: 5 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8154
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10603_length_8154_cov_4.213360"
                     /isolate="AMR_MDS_3064"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..829)
                     /locus_tag="P5123_08610"
     CDS             complement(<1..829)
                     /locus_tag="P5123_08610"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRX16361.1"
                     /translation="MSNFHKLLFLFSLFCFFSCDSSDSNTDKTPEVNQNIGNNVTAVV
                     VTNINGKSTGLAESVGTLPSPMPKENSIIFHTESTPTTAIRGRHNLTNYSNIYDSNPP
                     KRFAKTSRGLSQLDNNHTFWALDKNDDPIQINANLEYGSSTSKCLIYVADDYSGSFTN
                     WDSIGLFFDQTVSPRMLTGFGSPTDVDSNGKVIILYYDMDYPTYLGFFNPIDLFSSED
                     ANSIGRKSNEMEIFYMNYTWGTSSGSPEPDPDHPEMLRTLPHEFQHMIYFDEHYNRGV
                     "
     gene            1264..2958
                     /gene="fliF"
                     /locus_tag="P5123_08615"
     CDS             1264..2958
                     /gene="fliF"
                     /locus_tag="P5123_08615"
                     /inference="COORDINATES: protein motif:HMM:TIGR00206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009431 - bacterial-type flagellum basal
                     body, MS ring [Evidence IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal-body MS-ring/collar protein
                     FliF"
                     /protein_id="HRX16362.1"
                     /translation="MDFVKQMVSQLKDVFDKLTTAKKAIVGAVCAVFVISFIALFSIS
                     SETPKSVLFTELSAADFGQVTKKLEELGYHYTTTGTTAIHVDPKDREIIMTRLAQEDM
                     IPKGIPGWQLFDVSKWTETDREIDVKYMRALRGSIQKHIESLKPIEKADVEIAMTSDD
                     LYSSPEATYTAAVTVHYAPGYETIGQKTIKGIMYLVSRAVGSKLKPENVTVTNAEGKI
                     ISDFDDDFNKAKEELTIIEQRRKIKERDRVRLLKDIHDGLERIFPDRVQIVSLNLEYN
                     WDEVTEDIKEHFPVEMVPDNPNTPYSERVVKDSLVVSEKTVDEKFQGHGWNPEGPAGT
                     ESNRPPGYKAADDQYAKYEKNENILNHKVNERNKKIIREPYEIAGIAVSIAIDGIQDL
                     PKKPDGSYDLDPSKDPIQIPLTPEELKKAEEIVKAAIQYNQIRGDQVAVENIMFDHSK
                     KWAGIRDEHYRKEQMKRLALAALIGLFALFLGFILFQAIKKELERRRRIREEQLALEQ
                     QRMREAALRAAEEEGVDVELSLEDRARLELQENAINLAKERPDDVAQLLRTWLAEE"
     gene            3099..4115
                     /gene="fliG"
                     /locus_tag="P5123_08620"
     CDS             3099..4115
                     /gene="fliG"
                     /locus_tag="P5123_08620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000932791.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009433 - bacterial-type flagellum basal
                     body, C ring [Evidence IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA];
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar motor switch protein FliG"
                     /protein_id="HRX16363.1"
                     /translation="MLQSKKTQLTGRQKAAIFLVTLGSEVSSEIFKHLREDEIEQLTF
                     EIARIDKVEPSEKDQVLMEFQELMMAQEFITNGGIEYAREVLERALGTQKSIDIVNRL
                     TSSLQVRPFDFIRRTDPSHLLNFIANEHPQTIALILAYLDPQKAAQILSSLNHDIQAD
                     VAKRIAKMDRTSPDVLREVERVLERKLSTLASEDYTSAGGIDAIVEVLNNVDRTTEKI
                     IIEALEEDDPELAEDIKKRMFIFEDIVLLDDRSIQKVLREVDNNELGKALKGVDADVQ
                     EKIFKNMSKRAASLLKEDMDFMGPLRIRDVEESQQKIVNIIRKLEDSGEIVVARSGEE
                     ELVE"
     gene            4158..5084
                     /gene="fliH"
                     /locus_tag="P5123_08625"
     CDS             4158..5084
                     /gene="fliH"
                     /locus_tag="P5123_08625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008592071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009288 - bacterial-type flagellum
                     [Evidence IEA];
                     GO_function: GO:0003774 - cytoskeletal motor activity
                     [Evidence IEA];
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar assembly protein FliH"
                     /protein_id="HRX16364.1"
                     /translation="MAKLVFKPMEIHQTVIPKQLELPDKYQKSLMSDDEYKEFEVDAD
                     GNPVDVYQGPSIEEMEAELERNRREAEEDSRRMLEEARLEAERIVEAGKNDAFNELKI
                     AREQIKNEMEQQRKDSDREIERGKFEAEKMIKDAELKVSIIEHDAYKKGYDAGREEGY
                     KEGESEVMRLIDRLGTIVSTAVDIRDDIIKSSEKMMTEMILMIARKVIKDEIVERREV
                     VINNIKEAIRRVKDRDRIDIRVNFADLDMTTAHKDELIKMMESLKKVNIYEDSRVDRG
                     GCIIETDVGSIDSRISTQLDTIEEAIRNTSTM"
     gene            5102..6490
                     /locus_tag="P5123_08630"
     CDS             5102..6490
                     /locus_tag="P5123_08630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013120407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FliI/YscN family ATPase"
                     /protein_id="HRX16365.1"
                     /translation="MIRVLPHEKVDILSKYMKVLEDVDCVRLTGYVDKIQGLSIESVG
                     PNVRFGDVCRIMTGKKEFIYAEVVGFKAKDRPVLMPIGDMNGVLPGADVISLGSSLMA
                     PVGDEILGRVIDGIGMPIDGKGPIITSQKYPIEGTPVNPLQRCNVEKPLSTGIRAIDG
                     LMTVGRGQRIGIFSGSGVGKSTTLGMIARYTDADINVIALIGERGREVRDFVYKELGE
                     EGLKRSVVIVATSDQAPMMRIRGAYLAHSIAEYFRDQGKDVNLLLDSVTRFAMAQREK
                     GLAAGEPSASKGYPPSVFNMLARMMERSGNGINGSISAFYTVLIEADDMNDPIADAVR
                     GILDGHIILDRSIANKGHYPAIDVLASLSRCMKDVAAEEHQALASELRGLLAAYKDVE
                     DMVMLNTYARGTNPQADRAINMINDINSFLRQKVADGSSIDKAVAQLHALLDAPQLSR
                     ARRSDYVSSTTR"
     gene            6504..6971
                     /gene="fliJ"
                     /locus_tag="P5123_08635"
     CDS             6504..6971
                     /gene="fliJ"
                     /locus_tag="P5123_08635"
                     /inference="COORDINATES: protein motif:HMM:TIGR02473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009288 - bacterial-type flagellum
                     [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0003774 - cytoskeletal motor activity
                     [Evidence IEA];
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA];
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar export protein FliJ"
                     /protein_id="HRX16366.1"
                     /translation="MKKFSFRLQKLLDIKMAFEKKIQHELAREIGKQNLLREKQRVLK
                     EKIEQQQHAFHESMSRTGGNFNDLKAFHRYSQYADMVINSSEHDIMAMQPKINEIRGR
                     LLEASKERRSIEKLKEKKHDEWKYAVKRAEEVEYDEINMRIFHDQQRSKVNDI"
     gene            6961..7356
                     /locus_tag="P5123_08640"
     CDS             6961..7356
                     /locus_tag="P5123_08640"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRX16367.1"
                     /translation="MTSDMVNVLNRISELQKRFGLKREQLSGDAFKDSIKHKDNGSNN
                     DNLSMVLPSETVSIINAKNNMLIKNIVGEDYNERLQNKNINILDSINKSDEADIFLNN
                     KEMMINKAVKAYNNQNKSETNSNNVMEME"
     gene            7360..7956
                     /locus_tag="P5123_08645"
     CDS             7360..7956
                     /locus_tag="P5123_08645"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRX16368.1"
                     /translation="MRVSEKSKVIYLVLLIFFLLASGVFWLDYLGLVHLSRFYRQNFV
                     KSSESVLYADDDEPSLIRREEFIKEQDNLKDRIADLDKREALILEKEKELNKEEERLQ
                     EIARGLDLEKKKFEDEKEQYTGYRKNVVDLAQKIQAMPPESSVDIMINWEDPLIIDVL
                     RQMDQNAEEAGQVSITSYLISRMPKEKASRIMYLMTQL"
BASE COUNT         2593 a         1341 c         1878 g         2342 t
ORIGIN      
        1 tgacaccacg gttataatgt tcatcaaaat agatcatatg ctggaactca tgaggaagag
       61 tacgaagcat ttcggggtga tctggatcgg gctcagggct tcccgaggat gttccccaag
      121 tgtaattcat atagaaaatt tccatttcat tggattttct acctatcgaa ttagcatctt
      181 cgctagaaaa caaatcgatt gggttaaaaa accctaggta ggtggggtaa tccatgtcat
      241 aataaagaat aataactttt ccattactat ctacatcggt aggggaaccg aaacctgtga
      301 gcattcttgg tgatactgtc tgatcaaaaa aaaggccaat tgaatcccag ttagtaaaag
      361 atccggaata atcatctgct acatatatca agcatttgga tgtagaagaa ccatattcta
      421 aattggcgtt gatctggata ggatcgtcgt ttttatctaa agcccaaaat gtgtggttgt
      481 tatctaactg gctaagtcct cgtgatgttt ttgcaaatcg ctttggtgga ttagagtcat
      541 agatattaga ataatttgtt aagttgtgac gtcctctaat tgcagtggtc ggagttgatt
      601 ctgtgtgaaa aattattgag ttctcttttg gcatgggcga tggaagggtg ccaacagatt
      661 ctgcgagtcc tgtacttttg ccgtttatgt ttgtaactac gacagctgta acgttattgc
      721 caatgttttg atttacttca ggagttttgt ctgtgttact atcagatgaa tcacatgaaa
      781 aaaaacaaaa taatgaaaaa agaaataata atttgtgaaa gttgctcatt ttattctcca
      841 aatgaaaaat tatgaatatt agtttataaa actgttagta aatattcatg atgaaagtta
      901 atatatacta tacataaata aattaacagc atcaattggt ttatgtattt taaaccgttt
      961 tatgaacgtg atctaataac gtttttcatt tttttaatca tagcaattat ttaacaagaa
     1021 ttttattaag tattatcaat attttaagta aatttgtaat tttttctggt ttttgaccaa
     1081 aaaaagagat tatgcccctt tttttcattt cgtgtgcgct gaaaaagcga attatcgttt
     1141 acaaatagat agatgtatat tattattgca ttatgtctca ttaaattcgg taatcaccta
     1201 gttttcttgt ttgttttgca ttgcgcttgg gacatcttat acagtttttc ggggggaaat
     1261 tcgatggatt ttgttaaaca gatggtcagt cagttgaagg acgtttttga taaactgact
     1321 actgcaaaaa aagctattgt cggtgcggta tgtgctgtct ttgtgatttc gtttattgcc
     1381 ctatttagta ttagtagtga aactccaaaa tcggttcttt ttacagaact gtctgccgcc
     1441 gattttggcc aggttactaa aaagcttgaa gagttaggtt atcattatac aacaaccggg
     1501 actacggcta ttcatgtcga tccgaaggat cgcgagataa taatgacgcg tcttgctcaa
     1561 gaggatatga tccccaaggg tataccggga tggcagcttt ttgatgtaag taaatggacc
     1621 gaaacagatc gtgaaattga tgttaagtat atgcgtgcgt taagggggtc catacagaaa
     1681 catatcgagt cgttaaagcc tattgaaaag gccgatgtcg agatcgctat gacaagtgat
     1741 gatctctact cctctcccga agcaacttat accgccgctg taactgttca ttatgctcct
     1801 ggatacgaga ctatcggtca aaagacaata aaaggtataa tgtatcttgt ttcgcgcgca
     1861 gtaggaagta aactgaaacc tgaaaatgtt accgttacta acgctgaagg taagattatt
     1921 tcggattttg atgatgattt taataaagca aaagaagaac tcacgataat tgaacaacga
     1981 cgcaaaataa aagagcgtga tagggtaagg ttacttaaag acatacatga cggattagag
     2041 agaatttttc cggatagagt acagattgta agccttaatc tggaatataa ctgggacgag
     2101 gtaacagaag atataaaaga gcatttccct gtcgaaatgg ttcctgataa tcctaatacc
     2161 ccatattctg aaagagttgt aaaagattca cttgtcgttt ctgaaaagac tgttgatgag
     2221 aaatttcagg ggcatggctg gaaccctgaa gggcctgcag gcacagaatc aaacagacct
     2281 ccagggtaca aggcggctga tgatcaatat gcaaagtatg aaaaaaatga aaatatactt
     2341 aaccataagg ttaacgaaag aaataaaaaa atcataagag agccttatga aattgccggt
     2401 attgctgtct ctatagcaat agatggtata caggatttgc caaaaaaacc ggatggaagt
     2461 tatgatcttg atccttcaaa agatcctatt caaataccat tgacacctga agaattgaaa
     2521 aaagctgaag aaatagtaaa ggctgccata cagtataatc agatcagggg cgatcaggtt
     2581 gctgtagaga atattatgtt tgaccattca aagaaatggg cgggcataag ggatgagcat
     2641 tatcgaaaag agcagatgaa acgtttggct cttgctgcgc ttattggcct atttgcgttg
     2701 ttcctgggat ttattctttt tcaggcaatc aaaaaagagc tggagcgccg tcgtcgaata
     2761 cgcgaagaac agcttgctct tgagcaacaa cgtatgcgtg aagccgcatt aagggctgcc
     2821 gaggaagagg gtgtggatgt tgagctctca cttgaggatc gcgcacgact tgaacttcag
     2881 gaaaacgcaa ttaatcttgc caaagagcgt cctgatgacg tagcacagct tttaagaaca
     2941 tggcttgctg aagaatagat gtaaaattgc cgataaacaa ttgagggaaa ctctccggga
     3001 gacggaatat ccgttttccg gtttttctgt aaatacatac gttactctgt aatatttatc
     3061 tcttgatata agtttgcgga gcattataat ggatcattat gttacaatct aaaaagacac
     3121 agctgaccgg ccggcaaaag gctgctatct ttcttgttac tctcggatcc gaggtgtcat
     3181 ccgagatatt caagcatctg cgcgaggatg aaattgagca gctgactttt gagattgccc
     3241 gtatagacaa ggtagaacct tccgagaagg atcaggtact gatggaattc caagagctta
     3301 tgatggctca ggagttcatt accaacggtg gtattgaata tgctcgtgaa gttctagagc
     3361 gtgcgctagg tactcagaag tccattgata ttgttaatcg acttacctcg tcattgcagg
     3421 ttcgtccctt cgattttatc cgccgtacgg atccgagtca cctgttaaac tttattgcta
     3481 acgagcaccc gcagaccata gctcttattc ttgcgtatct tgacccccaa aaagcggcac
     3541 agatactatc ctcactgaat catgatattc aggcagatgt agcaaagcga attgcaaaga
     3601 tggaccgaac atcgcctgat gttttacgtg aggttgagag ggttctggaa cgcaaactat
     3661 cgactcttgc gtctgaagac tatacatcgg ccggtggtat tgatgcaatt gttgaggttc
     3721 ttaataatgt ggatcggaca acagagaaaa tcattatcga agcacttgaa gaggatgatc
     3781 cagaacttgc agaagatatc aaaaaacgta tgtttatatt cgaggatatt gttcttctgg
     3841 acgaccgttc tattcagaaa gttttgcgag aagttgataa caatgaacta ggtaaagctc
     3901 tcaaaggggt tgacgctgat gttcaagaaa aaatattcaa gaacatgtct aaaagggctg
     3961 cttcattatt gaaagaggat atggatttca tggggcctct gcgtatacgt gatgtggagg
     4021 agtctcaaca aaaaattgtt aatattatca gaaaacttga agactcgggt gaaattgtcg
     4081 ttgcccgtag tggggaagaa gaacttgttg aataaacagt gtaacagcag agttgattat
     4141 atgtcaggag gtgattcttg gctaagttag tatttaaacc gatggaaatt caccagacgg
     4201 ttataccgaa acagcttgaa ttgccggata agtatcagaa gagtcttatg tccgacgatg
     4261 agtataagga gttcgaagtc gatgctgacg gtaatccggt agacgtatat cagggaccct
     4321 ctattgaaga gatggaggcc gaacttgaac gtaatcgccg tgaagctgaa gaagatagtc
     4381 gacgcatgct tgaagaagct cgtcttgagg ccgaacgtat agtcgaggct ggtaagaatg
     4441 atgcatttaa tgagcttaag attgcccgtg agcagatcaa aaacgagatg gaacagcagc
     4501 gaaaagattc tgatcgtgag attgagcgtg gtaagtttga agctgaaaag atgataaaag
     4561 atgccgaact taaggtctca ataatagaac atgatgccta taaaaagggt tacgatgctg
     4621 gacgagaaga gggatacaaa gagggtgaat ccgaagtaat gcgccttatc gatcgtctgg
     4681 ggacaattgt ttcaactgca gtcgatatac gtgatgatat tatcaaatca tctgaaaaga
     4741 tgatgactga aatgattcta atgattgccc gaaaagtcat taaagatgag attgttgaac
     4801 gacgtgaagt tgttattaat aatattaaag aagcaatacg acgagttaaa gaccgtgacc
     4861 gtattgatat acgtgtgaac tttgctgatc ttgatatgac aacagctcat aaggacgagc
     4921 taatcaaaat gatggagtcg cttaagaagg tcaatattta cgaagattct cgtgtggatc
     4981 gcggtggatg tattatcgag actgacgttg gttccattga ttctagaata tcgactcaac
     5041 tggatacaat tgaggaggca atccggaata caagtactat gtagagccgg tttaaaaaaa
     5101 catgattaga gttttacctc acgaaaaagt agacatactt tccaagtata tgaaagtcct
     5161 tgaagatgtt gattgtgtcc gattaacagg atatgttgac aagatacagg gattgtcaat
     5221 tgagagcgtg ggacctaacg ttcgttttgg tgatgtgtgc cgtattatga caggcaaaaa
     5281 agagtttatc tatgctgagg ttgtggggtt taaagccaag gatcggccgg ttcttatgcc
     5341 tattggcgat atgaacggtg ttcttccggg tgccgatgtg atttctctag gatcttcatt
     5401 aatggctcca gttggtgatg agatactggg acgggttatt gatggtatag ggatgcctat
     5461 agatggcaaa gggcccatca ttacttcgca gaaatatcca attgagggaa ctcctgtaaa
     5521 tccgctacaa agatgtaatg tagaaaaacc gctttctaca ggtattagag cgattgatgg
     5581 actaatgact gttggccggg gacaacgaat tggtatcttc tctggttctg gtgttggaaa
     5641 gtcaacaaca cttggaatga ttgcacgata tactgatgct gatataaatg taattgcact
     5701 tataggcgag cgtggacgtg aagttcgtga ttttgtttac aaagagttag gtgaggaagg
     5761 gcttaaacgt tcagttgtta tagtggctac ttctgatcag gctcccatga tgcgtatccg
     5821 tggagcatac cttgctcatt ccattgcgga atatttccgc gatcagggaa aagatgtaaa
     5881 tttacttctg gattctgtta cccgttttgc tatggctcaa cgcgaaaaag ggctggctgc
     5941 aggtgaaccg tctgcatcga aaggttatcc gccttctgta tttaacatgc tggcgcgaat
     6001 gatggaacgt tcaggtaatg gaataaacgg ttcaatatcg gccttttaca ctgtgcttat
     6061 tgaagctgat gatatgaatg atccgatagc tgacgcggtt aggggaatac ttgatggaca
     6121 cattattctt gaccgttcaa tcgcgaataa aggacattac cctgcaatag atgttcttgc
     6181 ctctttatca agatgtatga aggatgttgc agctgaagag catcaagctt tagcatcgga
     6241 gctgcgtggt ttgctcgcag cttataagga tgtcgaagat atggtgatgt taaataccta
     6301 cgcccgtgga actaatccgc aggcagatcg tgcaattaat atgataaatg atataaacag
     6361 tttcttgaga caaaaagttg cggacggatc ttctatagat aaagctgttg ctcagttgca
     6421 tgctttattg gacgctccac aactttcgcg tgcccgcaga tcggattatg tctcttcaac
     6481 gacaagatga tagagaatag accatgaaaa agtttagttt taggttacaa aaacttttgg
     6541 atataaaaat ggcattcgaa aagaaaattc agcatgaatt ggcgagagag attggcaaac
     6601 aaaatctttt acgtgaaaag cagagggttt taaaagaaaa gatagaacag cagcaacatg
     6661 cttttcatga atcaatgtca agaacggggg gaaactttaa cgatctaaaa gcttttcacc
     6721 gttattcaca gtatgcagat atggttatta attcgtctga gcacgatatt atggctatgc
     6781 agcctaaaat taatgagata cgcggtcgcc tcttggaagc atccaaagaa cggcgttcga
     6841 tagaaaaatt aaaagaaaaa aaacatgatg agtggaaata cgctgtaaaa agagctgaag
     6901 aagttgaata tgacgagatt aatatgcgca tttttcatga tcagcaaagg agtaaagtaa
     6961 atgacatctg atatggtaaa tgtattaaac agaataagtg agcttcaaaa acgattcggt
     7021 cttaaaagag aacagctttc tggtgatgct tttaaagata gtattaaaca taaagataat
     7081 gggtcaaata atgataattt atccatggtt ttaccatcag aaactgtatc gattattaat
     7141 gccaaaaata atatgctaat aaaaaatatt gtcggtgaag attacaacga gcgtttacaa
     7201 aataaaaaca taaatatatt agacagtata aataaatctg atgaagcaga tattttttta
     7261 aacaataaag agatgatgat taacaaggct gttaaggcat ataataatca aaataaatcc
     7321 gaaacaaatt caaataatgt tatggaaatg gagtaacaaa tgagagtatc tgaaaagtct
     7381 aaagttatct atcttgtact tttgatcttc ttcttgttgg cttctggagt gttttggctt
     7441 gattatcttg gtctggttca tttaagtcgt ttttatcgtc agaactttgt caagtcttcg
     7501 gaatctgttt tatacgctga tgatgatgaa ccatcactga ttcgtcgtga agaatttatc
     7561 aaagagcagg acaatttaaa ggatcgtatt gctgatcttg acaaacgtga agcattaata
     7621 cttgaaaaag aaaaagagct gaataaggaa gaagaacgtc ttcaggaaat agctcgtgga
     7681 ctagatcttg aaaaaaagaa gtttgaagat gagaaagaac agtataccgg ttacagaaaa
     7741 aatgttgtag atcttgctca aaaaattcag gccatgccac ctgaatcatc ggttgatatt
     7801 atgataaact gggaagatcc tctgattatt gatgtattac ggcagatgga tcaaaatgca
     7861 gaagaagcag gacaggtcag tattacatct tatttgattt ctcgtatgcc aaaggagaag
     7921 gcaagtcgta ttatgtattt aatgacgcaa ctgtaaaaca gctaggcttc gagagcctca
     7981 gccagcgtgg ggtcgcttgg cttcgagggc ctcagccagc gagaggacgt ttggcttcga
     8041 gagcctcagc cagcgagagg acgtttggct tcgagggcct catccagcgc gaaggccgtt
     8101 tggcttcgag tgcctcagcc agcgcgaagg ccgcttggct tcgagtgcct cagc
//
LOCUS       DAOTGZ010000120         8129 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_3064
            NODE_10641_length_8129_cov_5.328090, whole genome shotgun sequence.
ACCESSION   DAOTGZ010000120 DAOTGZ010000000
VERSION     DAOTGZ010000120.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868088
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 8129)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8129)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:22:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,785
            CDSs (total)                      :: 2,751
            Genes (coding)                    :: 2,732
            CDSs (with protein)               :: 2,732
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 12 of 19
            Pseudo Genes (internal stop)      :: 5 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8129
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10641_length_8129_cov_5.328090"
                     /isolate="AMR_MDS_3064"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..387
                     /locus_tag="P5123_08650"
     CDS             <1..387
                     /locus_tag="P5123_08650"
                     /inference="COORDINATES: protein motif:HMM:NF021454.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2167 domain-containing protein"
                     /protein_id="HRX16369.1"
                     /translation="TNNLEWGIEFSSNGNQVINYNIRYLGRKGVMEVILVCNPDQLAN
                     AIEETQTLLVSYSFTKGNKYSEFVSGDKMAKYGLSALVAGGTVAAAAKSGLLQRFWKI
                     ILVAFAAVGGFIKKFFGRIFNRNKQQ"
     gene            408..1496
                     /locus_tag="P5123_08655"
     CDS             408..1496
                     /locus_tag="P5123_08655"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRX16370.1"
                     /translation="MTSNYQLFLLLLAYYVFECFVSINKKGSLIGKNIVYDFLNRKGV
                     FQFGKKLYLFAGFFSFGRNIYVLMPAALTLAEDGVLTDCLDSCKKFYYDSLESVSVEG
                     SFLVLNGSSVDCGSARYALYCKNIVNDCKTLDFKKRSDYIKKFYNKSSNVPEISDVIK
                     AYKSVSRFISVIASLLFITVFIVFPVLVFRNYFEAVWINFLVVYLVLILFQIILGTYV
                     LRKIYKASLSRVIGVSLTLLVSPLSAIRLVSHLSKDLLVQYHPAAVYVAANSQESSEK
                     YVSKYFRYIYIKLQKEQDENKKEYLAKENKTILRIIEQFGIDPNKLWHEPEKESSEIV
                     SFCPVCLEQYLKADAYCDDCRLMTHQYK"
     gene            1519..2376
                     /locus_tag="P5123_08660"
     CDS             1519..2376
                     /locus_tag="P5123_08660"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRX16371.1"
                     /translation="MINWKRTFLLLFFLTVACNPLPKKDKYPDVPLFEEAVSDHNIFE
                     KISSGSNQFEIVFLKDNRIMFLPVLSKYTSLQGILKISELNGHNIRSVNIEDGEPLYI
                     DTEGNLHYKNTKYIYPDYKQTQAYETIIISDILRDKKRKLDELYKDDESKASKIFDEY
                     QKELSNKYQIEPCESSLNSLFAGESCDLYFFHNDRLVIRQNQLYKNEFAKETKPLKEF
                     DDPVITGYKGSGGDYGVPLPVYMRYYEIGDNIKFKEKDYPYPVQIKLNGDQFVYSARY
                     GLYRVKLGR"
     gene            complement(2365..3309)
                     /locus_tag="P5123_08665"
     CDS             complement(2365..3309)
                     /locus_tag="P5123_08665"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRX16372.1"
                     /translation="MLGTITAQLLPVFLILAIGTLLRYFSLITEQTIDGLKNIIIKVS
                     LPAVLYLAFATMTLNLDFIILGIIIFCLCIVLYGMGIVIAPWSGPYKDFLKFLSTGFE
                     FGMLGAVFFGAAFGMKNMGYIAVIAIGHEIFIWFIYFTFIKNHNHTESSLKETAKGFL
                     TSPVIIGILAGILMNITGIHSLSANNSIAKGIIKTMNYLTGLTVPLILIVIGYGLRVK
                     LENVKNALIFSVSRLCVVITIAVAIDIILFQGILKLPKLYSAALYMFMLLPPPFILPL
                     FLNSGAKERDLINNTLIVYTVLSICAFAVYYIIFTLTT"
     gene            complement(3417..5711)
                     /locus_tag="P5123_08670"
     CDS             complement(3417..5711)
                     /locus_tag="P5123_08670"
                     /EC_number="3.6.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008779366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium-translocating pyrophosphatase"
                     /protein_id="HRX16373.1"
                     /translation="MLETIFWIIPGASVIALIFALLFYKQMMKHSEGNDTMQEIAQHV
                     RAGANAYLSSQYKVVFKVFIVLVILLTVLAFLNLQNPFVPFAFLTGGFFSGLCGYLGM
                     KTATNASARTAQGASESLNQGLKIAFRSGAVMGLVVVGFGLFDIAMWFWFLNEVIFTP
                     ENMKNGLEWASLTFVQAGVTEKGKMIEITVTMLTFGMGASTQALFARVGGGIFTKAAD
                     VGADLVGKIEAGIPEDDPRNPATIADNVGDNVGDVAGMGADLYESYCGSILATAALGA
                     ALPALTTAEQMHAIMSPMIVAAIGILLSIAGIFFVRTKEHATQKNLLTSLLLGTGGSS
                     SLILIALVILAYFNWISWGILGAVAVGLIAGVIIGQGTEYFTSDEFSPTKGIAGQGLQ
                     GPATLIIDGIAVGMFSTWLPVVTIVFGIVGSFGFAGGFTNFAMGVYGIGFAAVGMLST
                     LGITLATDAFGPIADNAGGNAEMSKLPSEVRERTDALDMLGNTTAATGKGFAIGSAAL
                     TAMALLAAYIEEVRLWIGKLAKASTDGVQQVGDYFFYLAGTPEPVVQKGQRAIEISSA
                     CIRDFSAAYDLSLFNPLLLSGLFLGAMMAFVFSSMTMKAVGRAACKMVDEVRRQFKEI
                     PGIMEHKNEPDYARCVAISTKGAQREMIVPSLLAIAVPVATGVILGVPGVIGLLAGGL
                     TTGFTLAVMLNNSGGAWDNAKKYIEKGNHGGKNGSAHHAAVVGDTVGDPFKDTSGPSL
                     NILIKLMSMVSVVMAGCTVVFSLF"
     gene            6091..6690
                     /locus_tag="P5123_08675"
     CDS             6091..6690
                     /locus_tag="P5123_08675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006209859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HRX16374.1"
                     /translation="MNNPDLEKRIAFIEELEKLKLVYRQNVAVDGSRQENSAEHSWHI
                     AVMAMVLSDYSDFKNIDLLKVLKMLLIHDIVEIDAGDTFLYDSEANKSKDADENLAAN
                     RIFGLLPEDTKNEFISLWKEFEKRESAEAKFAASLDGLQPIMNHYLSNGLGIIKHKLK
                     TNQIIEKKRYISDASKELWDYAMEMIQKSEDAGLYSKPD"
     gene            complement(6711..7871)
                     /locus_tag="P5123_08680"
     CDS             complement(6711..7871)
                     /locus_tag="P5123_08680"
                     /inference="COORDINATES: protein motif:HMM:NF025019.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HRX16375.1"
                     /translation="MSIKREITAELLHCSKEYPVITLIGPRQSGKTTLVKNVFFKHSY
                     VNLENPEIKKIAVDDPKTLLTHYPPPVIFDEIQNVPELLSWIQVIVDEKPDKKAQFIL
                     TGSHQMQLRQSVTQSLAGRTALLTLLPFSMLELSDYVNKTERSAMILNGFMPRLHDQN
                     IRRERFYRDYYLTYVERDVRKLISIENQNAFELFLRLLAGRVGNEINYTDISNQVGIS
                     SVQIKKWISILEASFIIFRLPPYYTNFGKRLTKSPKIYFIEVGLAAYLLGIENEQQLE
                     RDPAFGRLFENMLIAEALKQKLNIGKDPALYFFRDHHQNEVDLLYTVDGNTTPVEIKS
                     SRTYNSEFTKGIKYFQKISKTNNRGLVLYDGDLQPSTDIADFRNFRAFFTKT"
BASE COUNT         2630 a         1475 c         1554 g         2470 t
ORIGIN      
        1 acaaataatc ttgagtgggg cattgaattt tcgagcaacg ggaatcaggt aattaactat
       61 aatatccgtt atcttggtcg aaaaggcgtc atggaagtga ttctggtctg taatccggat
      121 cagcttgcta atgcaataga agagactcaa acgctacttg tttcatactc tttcactaaa
      181 ggtaataaat acagcgaatt tgtttctggc gataaaatgg ctaaatacgg cttgtcagct
      241 cttgttgctg gtggtactgt tgccgcagca gccaaaagcg gacttttgca gcgtttttgg
      301 aaaattattc ttgtagcttt tgctgcagta ggcggtttta taaaaaaatt ctttggacgt
      361 attttcaatc gtaataaaca gcaataataa tatggaacaa aagcataatg acttcgaact
      421 accaactttt tctcttactt cttgcttatt atgtctttga atgtttcgtt tctattaata
      481 agaaaggctc tcttatcggt aagaatattg tttacgattt tttaaaccgc aagggagtct
      541 ttcagtttgg aaagaagctt tatctgtttg ctgggttttt cagttttgga cgcaatattt
      601 atgtacttat gcctgctgca ctgactcttg ctgaagatgg agtcttgaca gactgtctgg
      661 atagctgtaa gaaattttac tatgattctc ttgagagtgt atcagttgaa ggctctttcc
      721 tcgttcttaa tggttcatca gttgattgtg gttcagcacg atacgcgttg tactgtaaaa
      781 atatagttaa tgattgcaaa accttagatt tcaagaaacg ctccgattat ataaaaaagt
      841 tttataataa atcttcaaat gttcctgaaa tttctgatgt gattaaggct tataaatctg
      901 tctcaagatt tatttctgta atagcctctc tacttttcat tacggttttt attgtctttc
      961 ctgttttggt tttccgaaat tattttgaag cagtatggat caatttttta gttgtttatc
     1021 ttgttctgat tttgtttcag attatacttg gtacttatgt attaagaaaa atttataaag
     1081 catcgctttc ccgagttatt ggtgtatctc ttacgttact ggtttctcct ctctctgcga
     1141 tcaggttagt atcacatctt tcaaaagatt tgctggtgca ataccaccct gcggcggttt
     1201 atgtcgcagc aaacagccaa gagagttcag aaaaatatgt atctaaatat ttccgatata
     1261 tttacataaa attgcagaaa gaacaagacg aaaataaaaa agagtatctt gcaaaagaaa
     1321 acaaaactat tttaagaata attgaacaat ttggaataga ccccaataaa ttatggcatg
     1381 aaccagaaaa agaatccagt gagattgtct ccttttgccc agtttgtctt gagcaatact
     1441 taaaagccga tgcctattgt gatgattgca gattgatgac acatcaatat aagtaaatac
     1501 tttagtggat aataatttat gataaactgg aaaagaacat ttttgcttct atttttttta
     1561 acggttgcgt gtaatccttt gcctaaaaaa gataaatatc ctgatgtccc tctttttgaa
     1621 gaggcggtat cagaccacaa tatatttgaa aagattagtt caggttccaa tcaattcgaa
     1681 attgttttct taaaagataa tcgcataatg tttttacctg ttttaagcaa gtatacctca
     1741 ttacaaggaa ttctaaaaat atctgaactg aacgggcata atataagaag cgttaatatt
     1801 gaagacggtg aacctctata tattgatact gaaggaaatc ttcactataa aaatacaaag
     1861 tatatctatc cagactacaa gcagactcaa gcctacgaga caattatcat aagcgatatt
     1921 ttacgtgata aaaagcgtaa acttgatgag ctctataaag atgatgaatc taaagcaagc
     1981 aagatattcg acgaatatca gaaggagctt tcgaacaagt atcaaattga accttgcgag
     2041 tcaagtctga acagcctatt tgccggcgag agctgcgacc tctatttttt tcataatgat
     2101 cgattagtaa ttaggcagaa ccagctatat aaaaatgaat ttgccaaaga aactaaaccc
     2161 ctaaaagaat ttgatgatcc tgttattact ggatataagg gaagtggagg agattatgga
     2221 gttcctttgc cggtttatat gagatattat gaaattggtg ataacataaa atttaaagaa
     2281 aaagattatc cgtatcctgt acagattaaa ttaaacgggg atcagtttgt atatagtgct
     2341 cgatacggat tatatagagt caaattaggt cgttaaagta aagataatgt aatagacggc
     2401 aaaggcgcag atgcttaata cagtatacac tataagagtg ttgttaatca aatccctttc
     2461 tttagcgccg ctgtttaaaa acaaaggcaa tataaatggg ggaggtagca gcataaacat
     2521 atatagagcc gcgctgtaaa gttttggtag tttaagaata ccctggaaaa gaataatatc
     2581 tatggcaacc gcgattgtga ttacaacaca aagacggctt actgaaaaga ttaatgcatt
     2641 tttgacattt tcaagtttaa cacgaagccc atagccgatt acaataagga ttaatggaac
     2701 agtaaggccg gtaagatagt tcattgtttt aatgattcct ttcgcgattg agttgttggc
     2761 agataacgaa tgaattcctg taatgttcat aagaataccg gcaagtatgc caatgataac
     2821 cggtgacgtt aagaagcctt ttgctgtctc ttttaaagaa gactctgtat gattgtgatt
     2881 ttttataaat gtaaaatata taaaccagat aaaaatttca tgacctattg cgattacagc
     2941 aatatagccc atgtttttca tgccaaaagc cgcaccgaaa aacacggcac ctaacatgcc
     3001 aaattcaaaa ccagtagata aaaattttag aaaatctttg tatggtcctg accagggagc
     3061 aattactata cccattccgt ataagacgat acataaacaa aagattatta taccaagaat
     3121 tataaaatca agatttaggg tcattgtggc aaaagcaagg tagagaactg ccggcagaga
     3181 tactttaatt ataatattct tcaatccgtc gattgtctgt tcagtaatca gcgaaaaata
     3241 acgtaaaaga gtacctatag caagtataag aaaaacaggt aaaagctgtg ctgtgatagt
     3301 ccctaacatg attgccctct aataattgtg gtcgaatatt ttggaagtcc ctcaaagttt
     3361 tgagagactt ccaaacgaaa gttattacaa tatactgcag ttaaagtaaa aatacattaa
     3421 aaaagtgaaa aaacaacagt acatcctgcc ataacaactg ataccatgga cataagcttt
     3481 attaaaatat ttaaagatgg gccagaggta tctttaaatg gatctccaac tgtatctccg
     3541 acaactgccg catggtgtgc cgaaccgttt ttaccaccat ggtttccttt ttcaatatat
     3601 ttcttagcgt tatcccaggc tccaccggag ttatttagca tcacggcaag cgtaaaacct
     3661 gttgttaaac cgccagctaa aaggccaatt actcctggta ctcccaaaat aactcctgtt
     3721 gcaacaggaa cagctattgc taataaagaa ggaacaatca tctcgcgttg tgcacccttt
     3781 gtcgatatag ctacacaacg ggcatagtca ggttcgtttt tatgttccat aatgcctgga
     3841 atttctttaa attgtcggcg tacttcatcg accattttgc aggcggcacg tccaacagct
     3901 ttcattgtca tagagctaaa aacaaaggcc atcatggctc ctaaaaatag accacttaac
     3961 aataaaggat taaaaaggga taaatcgtaa gcagctgaaa aatcacgaat acaagccgaa
     4021 gatatttcaa ttgctctctg gcctttctga actactggct cgggggttcc tgcaagataa
     4081 aaaaagtagt ctccaacctg ttgtacacca tcggttgaag ccttggcaag tttaccaatc
     4141 cataaacgaa cttcttcaat ataggctgca agaagagcca tcgcagtaag tgccgcagag
     4201 ccgatagcaa aaccttttcc agttgcagct gtcgtattgc caagcatatc aagggcgtcg
     4261 gtgcgttcac gtacttcaga aggcagtttt gacatttcgg cgtttcctcc agcattgtca
     4321 gcaatagggc caaaagcatc agtcgccaag gtaattccaa gagtcgaaag catacccacc
     4381 gcggcgaagc cgattccgta tacgcccatt gcgaaatttg tgaatccacc tgcgaatccg
     4441 aatgaaccta caataccgaa aactatggta acaacaggaa gccaggtcga aaacatccca
     4501 accgctatcc cgtcaataat caatgttgct gggccttgaa gaccttgtcc agcgatacct
     4561 tttgttggac taaactcgtc agatgtaaaa tattctgttc cttgaccaat tataacaccg
     4621 gcaattaaac caacggccac ggctcctaat attccccaac taatccagtt aaaatatgcg
     4681 agaataacaa gagctataag tatcagtgac gagcttcccc ctgttccaag caataacgac
     4741 gtcaaaagat ttttctgtgt cgcatgctct ttggttcgaa cgaaaaatat tcccgcaatg
     4801 gaaagaagaa ttccgattgc ggcaactatc attggtgaca ttattgcatg catctgctcg
     4861 gcagttgtta aagcaggaag tgctgctcca agagcggcag tcgcaagtat tgaaccacaa
     4921 tatgattcat aaaggtcggc acccattcct gcgacgtctc caacattgtc gcctacgttg
     4981 tcggcaattg tggccgggtt gcgcggatca tcttcgggta tgccagcttc aattttccct
     5041 acaaggtctg ctccaacatc ggcagctttt gtaaaaatac cacctccaac tcgcgcgaag
     5101 agagcctggg tcgaggctcc cataccgaat gttaacattg taacagtaat ttcgatcatt
     5161 ttaccttttt cggtaacacc tgcctgaaca aaggtgagac ttgcccactc aagtccgttt
     5221 ttcatgtttt cgggagtaaa gataacttca tttaaaaacc agaaccacat tgcaatatcg
     5281 aacagtccaa aaccaacaac aacaagcccc attacggcac cgcttcggaa tgcaatttta
     5341 agtccctgat tcaatgattc tgaagctccc tgtgctgtgc gggcagaagc atttgtagcg
     5401 gttttcattc caagataacc gcataagccg gaaaagaagc cccctgttaa aaatgcaaaa
     5461 ggaacaaacg gattttgcaa attcaagaaa gctaaaactg taagaagtat caccagtact
     5521 ataaatactt tgaataccac tttatactga ctgcttaaat aagcgtttgc tccagcgcgt
     5581 acgtgttgtg caatttcttg cattgtatcg ttgccttccg aatgtttcat catttgctta
     5641 tagaaaagta gtgcgaaaat aagtgctatt accgaagcac caggaataat ccaaaaaatt
     5701 gtttctaaca tgattaatta tttatcctta atataaaatt ttagtaacgt atatcattaa
     5761 cagtgttgta cgctataaca tagcatatta tactattcat cggacaatta taggttcatc
     5821 aagtattgtt ggagagtcct taaaaaatat ttaagttcaa atgcttgtta tggcacctgt
     5881 tattgatgtg agactataat tagcatttct aaacagcgtc aagtgattat tttataacta
     5941 tataatttga atggtgcaag attgtgttac aaataacttc tagaagtctg tataaaatat
     6001 tcattaatgg aaattattaa ttttcacttg tgtaaatatt agagatctgt aaaaagaaag
     6061 agcaaagtaa ttaattatta tggaggtcag atgaataacc ctgatttaga aaagcgcata
     6121 gcctttatcg aagagctaga aaaactgaaa ttggtttaca ggcaaaatgt tgctgtggat
     6181 ggttcgcgac aggaaaacag cgcggagcac tcctggcata ttgcggttat ggctatggtt
     6241 ttatctgatt actcggattt taagaatatt gatttgttaa aggttcttaa aatgctttta
     6301 atacatgata tagttgagat tgacgcaggg gatacttttt tatatgacag tgaggccaat
     6361 aaatcaaaag atgctgatga gaatttagct gcaaaccgta ttttcggact gttaccggaa
     6421 gataccaaaa atgagtttat atcactttgg aaggagttcg agaaaaggga atctgcagag
     6481 gcaaagttcg cagcctctct cgacgggctg caacctataa tgaatcatta tttatcaaac
     6541 ggcctgggaa tcataaaaca taaactaaaa acgaatcaga taatcgaaaa aaagagatac
     6601 atatcagacg cttcgaaaga gctttgggat tatgcaatgg agatgattca aaaaagtgag
     6661 gacgctgggt tatactcaaa accggattaa ctaattacat ttgagggcat ttacgtttta
     6721 gtaaaaaaag ctctgaaatt tctgaagtct gcgatgtcag ttgacggttg aaggtctcca
     6781 tcatacagaa caagcccccg attgttggtt tttgaaattt tctgaaaata tttgatgcct
     6841 ttggtgaatt cactgttgta ggttcttgaa gatttaatct cgactggagt agtgttgcca
     6901 tcaacagtat aaagcagatc aacttcattt tgatggtgat cacgaaaaaa atagagcgcc
     6961 ggatccttac caatattgag tttctgttta agagcttctg caattagcat gttttcaaat
     7021 agccgtccaa aagcggggtc acgttccagt tgctgctcat tttcaattcc cagaaggtag
     7081 gcggctaaac caacctcgat aaaataaatc tttggtgatt ttgtaagacg ttttccaaaa
     7141 tttgtataat atggtggtaa acgaaatatt ataaaagatg cttctaatat gctaatccat
     7201 ttttttattt gcacggaact tattccgacc tgatttgaga tatcagtgta attgatctcg
     7261 tttccgactc taccagctaa tagacgaaga aaaagttcaa atgcattttg attttctata
     7321 gaaataagtt ttcggacatc tcgctcgaca taggtcaggt aataatctcg ataaaaccgt
     7381 tctcttctta tattctgatc atgaagcctt ggcataaatc cattaagtat cattgccgat
     7441 ctttcggttt tatttacata atctgacaac tcgagcatcg aaaacggaag aagagtcaac
     7501 agtgctgttc taccggcaag tgattgagtt accgattgcc gtagttgcat ttgatgactg
     7561 cctgttaaaa tgaactgagc ttttttatca ggtttctcat cgactataac ctgaatccac
     7621 gacagcagtt cagggacatt ttgaatctcg tcaaatatta ccggtggagg atagtgggtt
     7681 aaaagtgttt tagggtcatc aacagcaatc ttcttgattt cagggttttc gaggtttaca
     7741 tagctatgtt taaaaaaaac attttttacc agagtagttt tgcctgattg tcttggacca
     7801 atcagggtaa ttactggata ctcctttgag caatgaagca gctctgctgt aatttctctt
     7861 ttaattgaca ttggacaaat ttaaactcaa agtttaaaat tgtccagctt ttttcatttt
     7921 ctaaatgaat aatcgaaaat tatggaattg taaatgggga cggaggtctt tatgaacaat
     7981 atttatgagt gataaggacg gtagcattta cctgatgttc gcagtgtgaa accttaataa
     8041 cagggacagt ggtattacaa cacctccgtc cccactaaga ctacgattgg cagcgtaaat
     8101 tttcagtaat ttagtaattt agaatcagc
//
LOCUS       DAOTGZ010000121         8108 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_3064
            NODE_10679_length_8108_cov_5.726562, whole genome shotgun sequence.
ACCESSION   DAOTGZ010000121 DAOTGZ010000000
VERSION     DAOTGZ010000121.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868088
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 8108)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8108)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:22:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,785
            CDSs (total)                      :: 2,751
            Genes (coding)                    :: 2,732
            CDSs (with protein)               :: 2,732
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 12 of 19
            Pseudo Genes (internal stop)      :: 5 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8108
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10679_length_8108_cov_5.726562"
                     /isolate="AMR_MDS_3064"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(89..589)
                     /locus_tag="P5123_08685"
     CDS             complement(89..589)
                     /locus_tag="P5123_08685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011341228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX domain-containing protein"
                     /protein_id="HRX16376.1"
                     /translation="MEEFFDVVDDNNRVIGRAARSECHGNPNLAHRVVHVLVFNSAGQ
                     LYLQKRSMTKDIQPGVWDTSVGGHLMEGESYFDAAIREMKEELAIENVDLEELYGYVL
                     RNSIETEFVTTYKTVYDGEINPDPVEITEGRFFDLKEIESLMGKGYFSSNFEDEFNLY
                     TEKYLK"
     gene            complement(598..1359)
                     /locus_tag="P5123_08690"
     CDS             complement(598..1359)
                     /locus_tag="P5123_08690"
                     /inference="COORDINATES: protein motif:HMM:NF012345.2"
                     /note="Members of this family of hydrolases with an active
                     site Cys residue belong to MEROPS family C26; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamyl-gamma-aminobutyrate hydrolase
                     family protein"
                     /protein_id="HRX16377.1"
                     /translation="MKRSLIVIMMLLFSCKPADNPSKQESEESFNTNETRIKKIAVIN
                     PAKDPLKLSMVKPVLEIIPSNGSVRTMAFHYKERNSFKSFKPDIIIVTGQSTPWNEYD
                     LSQFNYIREYIETEKVYALGICGGHQLFAMLWGGAVDLVKEASCDKSKGYSGCYKIKG
                     FVDVEIIGDRLGLFAGYPDKTIFYASHCEEIKRVPEEFMVLAKHILVKNYAIKHKLLP
                     VYGVQFHPELPANNPEQGRAVIDNFLKLAGCDIGE"
     gene            1509..1904
                     /locus_tag="P5123_08695"
     CDS             1509..1904
                     /locus_tag="P5123_08695"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRX16378.1"
                     /translation="MNIIEKNYKTLEKMQFLASLRENSYVRYKRPGMKDLHLNKMRDD
                     GKILHFSISHYDLDGGSLKADPDMEIIADWSKGIVKPLTYHDDFNETYKEIDENTMSN
                     HDLVNELSHFLDKWLTKIGESQYVIANKR"
     gene            complement(1958..2638)
                     /locus_tag="P5123_08700"
     CDS             complement(1958..2638)
                     /locus_tag="P5123_08700"
                     /inference="COORDINATES: protein motif:HMM:NF021567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2293 domain-containing protein"
                     /protein_id="HRX16379.1"
                     /translation="MGFKLEPNDLIEQKGGILTDRDGNPAVIPGGWVFLPAGDAVVTR
                     KTKAAGSFFVVRYRHGRKWFSKGIWADADIIEMAKKEVNQKRSTEDYKRRSEQVKKYR
                     QKKEQQYAVQFEQEIAAFLNFASVYKSLQDQLSKTISEYVVPVGSGTVARTKMIPIEK
                     RAESAVIAWLRHNITEYDNMAIPRQKGVRREIRRSLARASRDILEKYRKGEEIPEHCP
                     LKRACRGL"
     gene            complement(2655..3230)
                     /locus_tag="P5123_08705"
     CDS             complement(2655..3230)
                     /locus_tag="P5123_08705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013253211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rubrerythrin family protein"
                     /protein_id="HRX16380.1"
                     /translation="MAKIKGTKTEKNLLTAFAGESQARNRYTYYASKAKKDGYVQIST
                     IFEETANQEKEHAKRFFKFLEGGELMIEASFPAGVIGTTEQNLKDAADGEKHEWSEMY
                     PEFAKTAREEGFEEIAKVFEAVSVAEKQHEKRYRDLMNNINSGLVFKRDNVVVWRCRN
                     CGYLHEATEAPDLCAACAHPKAHFELLGENW"
     gene            3424..5073
                     /gene="hcp"
                     /locus_tag="P5123_08710"
                     /gene_synonym="priS"
     CDS             3424..5073
                     /gene="hcp"
                     /locus_tag="P5123_08710"
                     /gene_synonym="priS"
                     /EC_number="1.7.99.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012611155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016661 - oxidoreductase activity, acting
                     on other nitrogenous compounds as donors [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxylamine reductase"
                     /protein_id="HRX16381.1"
                     /translation="MFCHQCQETAQNKGCDKMQGVCGKKSDVANLQDLFIWTLKGISF
                     WGDMATQKNLYNEEAAFFIAKGLFMTITNADFSKDHFVQKINQAIKHRETVKKQLLEK
                     TEVSGDLPDYAVWTPANDNDILSKAESGTGGWLEIENEDIRSLKATILYGLKGMSAYA
                     EHGYQIIKGGEEIFKYIMEALSVIADENATQDDLISYVMKTGEYGVKAMAYLDDANSH
                     YGDTEISEVNLGVGTNPGILISGHDLVDLEELLEQTKDQQVDIYTHSEMLPAHYRPFF
                     KKYDHLRGNYGNSWYLQKDELKSFNGPALFTSNCLVPPDADKMDKIFTTGAVGFEGLA
                     YIPDRKPGDHKDFGPIIEMAKKCDAPVAIESGKIVGGFGHKQVLKLADKIIDAIKAGK
                     IKRFIVMAGCDGRHKSRSYYTEVAKELPKDTVILTAGCAKYRYNKLDLGDIDGIPRVL
                     DAGQCNDSYSLAVIALELQKAFKMADINDLPISFDIAWYEQKAVLVLLALLHLGVKGI
                     RLGPTLPGFLSPNIADFIIKSFELKGITNAKDDVQAMMAGK"
     gene            complement(5253..6257)
                     /locus_tag="P5123_08715"
     CDS             complement(5253..6257)
                     /locus_tag="P5123_08715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016289387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldolase catalytic domain-containing protein"
                     /protein_id="HRX16382.1"
                     /translation="MAKENEVNCTEMKRGSWLTYRPDIKVLDCTIRDGGLVNNFYFKD
                     EFVKAVYHTLIEMNVDYMEIGKIVNEKALAPSKYGIWNYCKEEDIRRVIGDNEDTDLK
                     IAVMADIGRTFKDDIRPKDESIVDMVRVACYIHQIPAALELIEDAHKKGYETTCNLMA
                     VSKCSKDDIKEALRLIVDSPVDVVYLVDSFGSYYSEQIRDLTRIYLEIAEAKGKVVGY
                     HGHNNQQLAYANTVETLICGTSYLDATIGGLGRGAGNCPLELLIGFLKNPKYHIVPLL
                     KFIQDYIVPLQKEIHWGYDIAYMLTGQLNEHPRSAIKFIDEKGTDYVDFFNSLCDVQE
                     "
     gene            complement(6467..6967)
                     /locus_tag="P5123_08720"
     CDS             complement(6467..6967)
                     /locus_tag="P5123_08720"
                     /inference="COORDINATES: protein motif:HMM:NF023540.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative metalloprotease CJM1_0395 family
                     protein"
                     /protein_id="HRX16383.1"
                     /translation="MNIDSRIIGRLYSNQVLVEKRPSDEGQLSESRKRMLDELKQRDN
                     HVRQHESSHANSPGVIQYGAPRFKYQLGPDGRPYAVGGEVTLGTMPSDNPQTARINAD
                     ALRKAALANGDPSPQDLAAASSAEAMKIEADINMISRSANIYKNMETEMNRPVYPPVS
                     SLNFYA"
     gene            complement(7102..7740)
                     /locus_tag="P5123_08725"
     CDS             complement(7102..7740)
                     /locus_tag="P5123_08725"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRX16384.1"
                     /translation="MIKKIMYFLMACLLMTAFLSCSDDESEDKASVIDDYPMGDVDVA
                     IAAEVMNVSMNKIDSAVNNAVMSGKRSATRATQTETINFTDSTGKIKVTGEVTFDAGD
                     ETELSDYSYFLTIEFDDYEEGGVILDGQVDYEMYYTFDVDTGQNFEYTYSGDFKILYK
                     GYRYEIEWDFEMSYVNFEYSITGSYTCNGETFGSEVLTGFVSDEESESEDWR"
BASE COUNT         2505 a         1774 c         1357 g         2472 t
ORIGIN      
        1 cggcagaaca atttcaaagt atattatgaa catccaataa ctttttaacg gattatcttt
       61 tctgggaatc cagtgtgatc ccatatttct atttcagata tttctccgta taaaggttaa
      121 attcatcttc aaaatttgaa gaaaaatatc cctttcccat caatgattct atctctttta
      181 aatcaaagaa tcttccctca gtaatttcta ccggatcagg attaatttca ccatcatata
      241 ctgttttata agtcgtaaca aattcagttt cgatactgtt tcttaaaaca taaccatata
      301 actcttcgag atctacattc tctattgcaa gctcttcttt catttctcga atagccgcgt
      361 caaaatagct ctctccctcc atcaaatgcc caccaaccga agtatcccaa actccaggct
      421 gaatatcctt tgtcattgac cgcttttgaa gataaagctg accggctgaa ttaaaaacca
      481 gcacatgaac taccctgtga gcaagattcg gattaccatg acactcacta cgagccgccc
      541 tacctataac cctgttatta tcatctacaa catcaaaaaa ctcttccatt tattctttta
      601 ctctcctata tcgcatcctg ccaactttaa gaaattatcg ataaccgcgc gtccctgttc
      661 gggattgtta gccggcaact ctgggtggaa ctgaacaccg taaaccggta ataacttatg
      721 cttaatggca tagtttttta caagaatatg tttggctaaa accatgaatt cttctggaac
      781 cctttttatc tcctcacaat gtgaagcata aaatatggtc ttatcgggat atcccgcaaa
      841 aagtcctaac ctatcaccaa ttatttcaac atccacaaag ccctttattt tataacaacc
      901 agaataaccc ttcgatttat cacaactcgc ctcttttaca agatcaaccg cccctcccca
      961 caacattgca aaaagctggt gtccgccgca aatgcccaaa gcataaacct tttctgtttc
     1021 gatatattcc cgtatgtagt taaactgcga aagatcgtac tcgttccatg gagtagattg
     1081 cccggtaaca ataattatgt ccggtttaaa tgatttaaag gagttccgct ctttgtagtg
     1141 aaaggccatg gttctaacag agccgttgct tggaattatc tcaagaacgg gtttaaccat
     1201 ggacagtttt aatggatctt tcgccggatt aattactgct atttttttaa ttcgagtctc
     1261 atttgtgttg aaactctctt ctgattcctg tttagaaggg ttatcggctg gtttgcatga
     1321 aaacagtaac atcattataa cgatcagcga tctcttcatg gtaatctccg ccgtaaaact
     1381 gcagattctt catatcaaat ctaaatacta tttgaataat cggataattt tacaaaaaaa
     1441 catgaaaaaa cttgacataa ataaccggta tactatacat atgtatagta cttaagtgag
     1501 gttaggcaat gaacataata gaaaaaaatt acaaaacgct cgaaaaaatg caatttctcg
     1561 catcactccg tgaaaacagt tacgttcgat acaaaagacc cggcatgaaa gacctgcatc
     1621 ttaacaagat gcgtgatgac ggtaaaatac tccacttctc tatctcacac tatgaccttg
     1681 acggaggttc acttaaagcc gatcccgaca tggagattat tgcagactgg agcaaaggaa
     1741 ttgtcaagcc acttacatac catgacgatt ttaatgaaac atataaggag atcgatgaaa
     1801 acactatgtc aaatcacgat cttgtaaacg aattaagcca ttttctggac aaatggctga
     1861 ccaaaatagg cgaaagccaa tatgtaatcg caaataaacg gtaagccccc caactgaccg
     1921 ttttttgata tcacactttc aaccccatgc aaatcctcta aagaccccgg cacgcccgtt
     1981 taagcgggca gtgttcgggg atctcctctc cttttctgta cttttcaaga atatcacgtg
     2041 aagctctcgc caaggatctt cttatttcac gtctgacacc cttttgtctg ggtattgcca
     2101 tattgtcata ttccgtaata ttgtgtcgta gccacgcgat tacggcgctc tcggcccgtt
     2161 tttctattgg tatcattttt gtacgggcta cggttccact tccaacaggt acaacatact
     2221 cactgatggt ttttgaaagt tggtcttgaa gacttttata aaccgatgca aaattcaaaa
     2281 aagcagctat ttcttgctcg aactgaaccg cgtactgttg ctcctttttt tgccgatatt
     2341 tttttacctg ttcacttctt cgtttataat cttcagttga ccgtttttga ttaacctctt
     2401 tttttgccat ttctataata tcagcatcgg cccagatacc tttggaaaac catttgcgac
     2461 catgcctgta cctaaccaca aaaaaagaac cggctgcttt tgtcttgcga gttacgacag
     2521 catcacctgc cggcaaaaaa acccaaccgc caggtataac tgccggatta ccatcccgat
     2581 cagtcaagat accgcccttt tgctcaatca aatcgtttgg ctctaattta aaacccattc
     2641 agattctacc gttattacca gttttctcca agaagttcaa aatgagcctt tggatgagca
     2701 caagccgcac aaagatctgg tgcctctgtt gcttcatgca aatatccaca atttctgcaa
     2761 cgccatacaa caacattatc acgtttgaac acaagacccg aatttatgtt attcatcaaa
     2821 tctctataac gtttttcatg ctgcttctct gcaacagata ccgcctcaaa tactttagca
     2881 atctcttcaa agccctcttc gcgagctgtc ttggcaaatt cgggatacat ctcggaccac
     2941 tcatgcttct ctccgtccgc agcgtctttt agattctgtt ctgtcgttcc gattacacca
     3001 gccggaaaag atgcctctat catcagctct ccaccctcta aaaacttgaa aaaacgcttg
     3061 gcgtgctcct tctcttggtt agcggtctct tcaaaaattg tggagatctg aacatatccg
     3121 tccttttttg ccttgctggc ataatatgta taacgattac gagcctgtga ttctcctgcg
     3181 aatgctgtaa gcagattctt ctcagttttt gttcctttga ttttagccat gtgttaatcc
     3241 tccgataaca gttggtaaat ttgagacatt tacataaact ctgaactatg atccttttgc
     3301 aagaattttg taaaaaaatt cttttgtaaa aattattaaa aactccataa aacttgattt
     3361 gggtcaatgt tcatatgata ttataggtta taatagagca aataacttat tcagggggta
     3421 tatatgtttt gtcatcaatg tcaggaaaca gcacaaaaca agggttgtga taagatgcag
     3481 ggagtctgtg gaaagaagtc ggatgttgcc aacctgcagg atctttttat ctggacactt
     3541 aaaggaattt cattttgggg cgatatggca acccaaaaaa acctttataa cgaggaagct
     3601 gcttttttta tcgcgaaggg tctttttatg accatcacta atgcagattt tagcaaagat
     3661 cactttgttc aaaagattaa ccaggcgatc aaacaccgtg aaaccgtaaa aaaacagtta
     3721 ctagaaaaaa ccgaagtaag cggagatctt cctgattacg ccgtctggac acctgctaac
     3781 gataatgata tcttgtctaa agcagaatca ggaaccggtg gttggcttga aattgaaaac
     3841 gaggatatcc gttctttaaa agcaactatt ctgtatggct taaaaggtat gtcggcatat
     3901 gcagaacatg gataccagat tattaaaggc ggcgaagaaa ttttcaaata tataatggaa
     3961 gcactctcag taatcgcgga tgagaacgca actcaggacg atcttatctc ttatgttatg
     4021 aaaaccggtg aatatggtgt caaagctatg gcctatcttg atgacgccaa ctctcattac
     4081 ggtgatactg aaatatcaga agttaacctc ggtgtaggta ctaatccggg gatattaatt
     4141 tccggtcacg atcttgtaga ccttgaagag cttctggagc agacaaaaga tcaacaagta
     4201 gatatttaca ctcatagcga gatgctgcct gctcactaca ggcctttctt taaaaaatat
     4261 gaccaccttc gcggtaacta cggtaactct tggtacttac aaaaggacga attaaagagt
     4321 tttaatggac ctgcactgtt tacgtccaac tgtcttgttc caccagacgc agacaaaatg
     4381 gacaagatat ttacaacagg agcggttggt tttgaggggc ttgcatacat tcccgataga
     4441 aaaccaggtg atcacaaaga ttttggacca ataatcgaaa tggcaaaaaa atgcgatgca
     4501 cctgtagcaa ttgaaagcgg aaaaattgta ggcggtttcg gccacaaaca ggttcttaaa
     4561 cttgccgaca agataattga cgctataaaa gcaggaaaaa ttaaacgctt tatcgttatg
     4621 gccggttgcg atggtcgcca caagtcaaga tcatattata ctgaggttgc gaaagagctg
     4681 cccaaagaca cagtgatact tactgccggt tgcgcaaagt accgttataa caaacttgat
     4741 cttggtgata ttgatggaat ccctcgggtt cttgatgctg gacaatgtaa cgactcttat
     4801 tcgttagctg ttatcgcgtt agaacttcag aaggccttta aaatggccga tataaacgat
     4861 ttgccaattt cgtttgatat cgcatggtac gagcagaagg cagtactcgt tttacttgcc
     4921 ttgttgcatc ttggagtaaa gggaattcgc cttggaccta cactaccagg atttttatca
     4981 ccaaacatag cagacttcat aataaagagc ttcgaactta aaggcatcac aaacgctaaa
     5041 gatgatgtac aggcgatgat ggccggaaag taaaaggcgg aaaacagaac cacataagca
     5101 ctaataaaaa agaaccgatt tacggttctt ttttattatc aagtgttttt agaaaagcga
     5161 attacaataa attaatcagc attctaatta ggataatttt tcgaagttcc ctagactacc
     5221 ataatatttg tttaccacaa attttagtgt acctactcct gaacatcaca tagactattg
     5281 aaaaaatcaa catagtcagt acccttttca tcaatgaact ttattgccga gcgtggatgc
     5341 tcattaagct gacccgtaag catataggca atatcgtaac cccaatgaat ctctttttgt
     5401 aaaggcacaa tgtaatcctg aataaacttc agaagcggaa caatatgata cttcggattt
     5461 ttcagaaaac cgattaaaag ctcaagagga cagttacccg caccacgacc taaaccaccg
     5521 atagtagcat caagatagct tgtaccacag atcaatgttt cgacagtatt cgcgtaggca
     5581 agctgctggt tgttgtgtcc atgataaccg accacctttc cttttgcttc ggctatttca
     5641 agatatatac gtgtaagatc acgtatctgt tcagaataat atgacccaaa actatccaca
     5701 agatatacaa cgtctacagg actatctaca attagtcgaa gtgcctcttt aatatcgtct
     5761 tttgaacatt tcgaaacagc catcaaatta caggttgtct cgtaaccttt tttgtgagca
     5821 tcttctatca attcaagagc tgccggaatc tggtgaatat agcaagctac ccttaccata
     5881 tcaacgatac tctcatcttt aggccgtata tcgtctttaa atgtacggcc tatatcggcc
     5941 ataaccgcaa tcttaagatc agtatcttcg ttatctccga taactcggcg aatatcctct
     6001 tctttacaat agttccatat tccgtatttg gagggggcaa gcgccttctc gttaacaatt
     6061 tttcctattt ccatgtaatc gacattcatt tcaataagtg tatggtaaac agccttaaca
     6121 aattcatctt taaaataaaa attatttacc aacccgccat cacggatagt acaatccaat
     6181 accttgatat ccggtcgata tgtaagccat gaaccgcgct tcatctcggt acagttcact
     6241 tcgttttcct ttgccattat ctacctcata atttttgatt cttgaaaaag tgattctgtg
     6301 aaaagaaaca ctttttcaat tcattaaaaa aaaggtgctc tgttttgcaa caaaagaaac
     6361 acaaatgcac aaaaaaacct aagacatatt aatgtttttt ggatatgtaa ttttgtcttt
     6421 attatgttta agatcgatat tattattttg agagtagatt atatgattat gcgtaaaaat
     6481 tcaaagagga aaccggtgga taaaccgggc gattcatctc tgtttccata ttcttatata
     6541 tgtttgccga acggcttatc atattaatat ccgcttcaat cttcatggct tcagcggatg
     6601 aagcagctgc cagatcctgt ggtgacgggt caccgttagc gagtgccgcc tttcgtaatg
     6661 cgtcggcatt tatgcgtgct gtctgtggat tatccgaagg cattgtacca agagtaactt
     6721 caccacccac agcataaggt cgcccatcag gccctaactg atacttaaaa cgaggtgccc
     6781 catattgaat gaccccgggc gaatttgcgt gtgacgattc atgctgcctt acatgattgt
     6841 cacgctgctt aagctcatca agcattctct ttctcgactc gcttaactgc ccttcatcag
     6901 aaggtctctt ttcgacaaga acctgattag aatataatct gccgataatt cgggaatcga
     6961 tattcatttt taccttttta aatgtttaat tactgtcaat atcggatttt attaccctgt
     7021 aaattaatta tgataaaaaa aacggcatct aatgataccg tttttttgat tgaattattt
     7081 ataaattaaa cacggcctct attaacgcca gtcttcactc tcactctctt cgtccgaaac
     7141 aaaacctgtt aatacttccg atccgaaggt ttctccatta caagtataag aacctgttat
     7201 cgaatactca aaatttacat aactcatttc gaaatcccac tcaatttcgt aacggtatcc
     7261 tttatagaga atcttaaaat caccgctata tgtatattca aaattttgac cagtatcaac
     7321 atcaaaagtg taatacattt cataatccac ttgaccatca agtattactc caccctcttc
     7381 ataatcatca aattcaatag taagaaaata agaatagtca cttaactcag tctcatcccc
     7441 agcatcaaaa gtcacttcac cggtaacctt tatcttgccg gtcgaatcgg taaaatttat
     7501 ggtttcagtt tgtgtcgctc ttgtagcgct tcttttacca gacatgacag cattatttac
     7561 agcactatca attttattca tcgatacatt catcacctct gctgctatcg ctacatccac
     7621 atcacccatt ggataatcat caataacact agccttgtcc tcagattcat catcactaca
     7681 agacaaaaac gctgtcatta atagacaagc catcaggaaa tacataattt ttttaatcat
     7741 tttaccacct ctttttccgg agctatctcc actttttgat tctaaccgca cttaatcata
     7801 cattataaca agcagatcaa ttgcggacac aataatcaaa ttattataga caataaccat
     7861 tatgtcaaat cattcattgt cagattatca attattagcc actgcaacct gtttcaacta
     7921 gtaatttata ataaatctgt ctatttattc gagccataaa agtccacgcg ggctctattt
     7981 accaatattc aactatagtc actttaatat tgacaatatc acattatttg gaataatggt
     8041 ataaaacaca tgtaacacaa cagatttaat cggagagttt atggaaacct taaacagtgc
     8101 tttaaaat
//
LOCUS       DAOTGZ010000122         8106 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_3064
            NODE_10683_length_8106_cov_5.998882, whole genome shotgun sequence.
ACCESSION   DAOTGZ010000122 DAOTGZ010000000
VERSION     DAOTGZ010000122.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868088
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 8106)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8106)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:22:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,785
            CDSs (total)                      :: 2,751
            Genes (coding)                    :: 2,732
            CDSs (with protein)               :: 2,732
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 12 of 19
            Pseudo Genes (internal stop)      :: 5 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8106
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10683_length_8106_cov_5.998882"
                     /isolate="AMR_MDS_3064"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(77..208)
                     /locus_tag="P5123_08730"
     CDS             complement(77..208)
                     /locus_tag="P5123_08730"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRX16385.1"
                     /translation="MAAVLAREAAKARIKDEEMAREDAKVRRREERIKKGHAKTRRR"
     gene            complement(241..1479)
                     /locus_tag="P5123_08735"
     CDS             complement(241..1479)
                     /locus_tag="P5123_08735"
                     /inference="COORDINATES: protein motif:HMM:NF023223.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2220 family protein"
                     /protein_id="HRX16386.1"
                     /translation="MKSSPKKRPVKKWTDLSDVKNKLIRVWERGSVLRFLLDPDDCNS
                     ISLPLRVSLVGPSATEMAQQYSHCRLWISEFTDKKSEFSFDIEWKEKVNRQIGKNSIP
                     SALLFHDIETIVQFISKKNELLLFKENIALLLDRLPDLKKWIIKKPLQLTDPDIGWKQ
                     CVSLLEWLLKNPQSSIYTRQIPVPDIDSKFIEKHRGLLLDLYLSLIEPGDNYDSGERK
                     SFEDFLGFKKKPILVRLKFLDSDLFINGLSEVSVRHDELDRLEIEPDNVFVIENDINA
                     LTFPMTRNSLVIFGRGYGFSYLKQIKWLERANIWYWGDIDTHGFVILDQFKNIFPTSR
                     SLMMDYQTLMRFRNKWVRESKPNKRALLNQLSKDEKKIYESLSSDLYGVNVRLEQEFV
                     DYTYVIECLRSLNFELIDVK"
     gene            complement(1463..4825)
                     /locus_tag="P5123_08740"
     CDS             complement(1463..4825)
                     /locus_tag="P5123_08740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012662773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HRX16387.1"
                     /translation="MIPELEFESDNSRAGFRLKTLELYNWGTFDKKVWSFSVDGENAL
                     VTGDIGSGKSTLVDAVTTLLVPSHRIAYNKAAGAESRERSLRSYVLGYYKHERSEAGY
                     GTKSISLRTTGTYSVILAVFYNQGYSQSVTLAQVFYQKEPTGQPSRFYVVADRQLSIV
                     EHFSNFGNDIKTLRKRLKNEHGIAEVCETFPPYGAVFRRRFGLANEQALELFHQTVSM
                     KSVGNLTDFVRGHMLEPFEIAPRIEMLIEHFDDLNRAHDAVRRARRQIDLLNPIIANL
                     KIYNEQSQLKNDHVRKRSGLRYYFTDIKKALLENRIERLCREADSLNKSVVNGNSLRE
                     KRQLERDRLKQDIAEKGGNRVEEIKQSIARLGRERDHKKNRAAEYQKLIKELDVDYPD
                     SEKMFYNIKDNISEKLTAVKNDEAEIQNNRVQLEVSLKELKDNKEEIEREIQSLEKRH
                     SNISRRQVQIRDALCAALGVSEEKLPFAGELIAVCEPEKLWEGAIERLLHSFGLSLLV
                     PEELYSSVCDWVDKENLRGRLVYFRVAGEFTNLIEAPDSLSLRSKILVNPESAFSGWI
                     ENEILKRYNFVCCENAEQFRHHAYALSINGQIKRSGNHHEKDDRYDIHDRSRYILGWN
                     NLKKIELLRLSKSDAEKKISDIELSISDLKRQQNNIDNQKRLMLMIDSYTDYEDINWK
                     PLVVEIADYQKQLEEFTSSSDVLASLQKDLGLLEKKMREEELKLDNLKSDLARNDEKQ
                     NQSKSYLQECQVQLETEKEFFVDPKELQEFVEEISDNKKITIESADRLEQKVRELIQS
                     KIDSIERKIKRLSENIIKDMHDFCREYPAETVETDASIDAGPEYNRILENLLADDLPR
                     FEKKFKELLNENTIREIANFQSQLNRECQDIKERIERINLSLAGIEYNRDRYIQLEAL
                     TNSDIEIRDFQHDLKDCTEGALAVNFDEQESEKRFGKVKDIIEKFRGRDDRSDQDKRW
                     TRKVTDVRNWFVFAASERWNEDNSEYEHYTDSGGKSGGQKEKLAYTVLAASLAYQFGL
                     EWGEVRSRSFRFVVIDEAFGRGSDESARYALELFRRLDLQLLIVTPLQKIRIIEPYVS
                     RLGFVSNPEGRESVLREITMEEYQKEKSEKQP"
     gene            complement(4822..5418)
                     /locus_tag="P5123_08745"
     CDS             complement(4822..5418)
                     /locus_tag="P5123_08745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012474179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4194 domain-containing protein"
                     /protein_id="HRX16388.1"
                     /translation="MITEENGTQFSSVLVKLLKGVIYSVDNDKLWDELLKRQSAVRDY
                     FKVLGLDVIIYEDEGFAWLKQTTAQEGADEIPRLMPRRQLSFSVSLLLAILRKKLVEH
                     DSSSGERRLIIERDDLLEDIKTFFPQGTNEVRFKQRFDTSINKIESMGFVRSIGEGGS
                     SVEVCRIIKGFVDASWLAEFDTRLQEYVKYAGVKEVNE"
     gene            complement(5415..6866)
                     /locus_tag="P5123_08750"
     CDS             complement(5415..6866)
                     /locus_tag="P5123_08750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012431128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3375 domain-containing protein"
                     /protein_id="HRX16389.1"
                     /translation="MDFDYYTLKRLFETHSSIRLLRADNAPFVIAFLHDVFIKNNIHE
                     IFHYQLLSRLDDFLYYLNNSEDEPRFSRNASAYLDEWSSDDRGWLRKYYRYGSDEACY
                     DLSPASEKVLVWIGKLSRKHFIGTESRLNIIFDLLKQIAYGAETDTNARIAELKKQRD
                     AINKEIDALERGEHVIADDAVLRDSYIQMESTARELLGDFREVEQNFRFLDRTVREKI
                     NSWSGNKGELIEEIFGDRDLILDSDQGRTFNAFWDFLMSPNSQEELTRLLENVFSIEA
                     IQKLKPDNRIKRIHYDWLEAGEHTQRRVASLSQQLRRYLDNQVYLENRRVIQILESIS
                     SNSLKVRDMLPSGDFMSIDSMTADIDLYMERPLFSPPIEINFDSQITSAETGDIDTSQ
                     LYDQITVDKVMLRKNINTFLLEKGQVSLQEITQRYKLQCGAAELIVYLVIASEDENVI
                     FDESVNETIEWEDDEGNIRRANCCRVVYCRGKS"
     gene            complement(6933..7115)
                     /locus_tag="P5123_08755"
     CDS             complement(6933..7115)
                     /locus_tag="P5123_08755"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRX16390.1"
                     /translation="MEYILSELIGAFKTTASKMIHLSGNPSFQWQKSYYDRIIRNNDE
                     LNRIRKYIIHNPQNLT"
     gene            complement(7223..8041)
                     /locus_tag="P5123_08760"
     CDS             complement(7223..8041)
                     /locus_tag="P5123_08760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013136700.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005634 - nucleus [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="KilA-N domain-containing protein"
                     /protein_id="HRX16391.1"
                     /translation="MAKISVLDKEITFFTQNKEDYICLTDMARFKDPERTDYIIQNWL
                     RNRSTIEFLGIWEQLYNLDFKPIEFEGFRNQAGLNSFVLTPKQWIEKTSAIGIISKSG
                     RYGGTYAHKDIAFEFASWISVEFKLYLIKEFQRLKDEELKQLGWDIRRNLTKINYRIH
                     TDAIKQNLIPPELSKEQINLVYATEADILNMALFGMTAKDWRDKNPDLKGNIRDFAEI
                     SQLVCLSNLENLNALFIQEGLPQNERLLKLNQIAISQIKLLTEDSGIKKLKDKD"
BASE COUNT         2358 a         1849 c         1284 g         2615 t
ORIGIN      
        1 ataacctcag tatctacctt aaaaaaatat tatccacttt gcgtagctct ttcgcatgcc
       61 ctttccctct cttatcttag cgccttcgcg tcttcgcgtg ccctttcttt atcctttctt
      121 cgcgccttcg caccttcgcg tcttcgcgtg ccatttcttc atcctttatt cgcgccttcg
      181 cggcttcgcg tgctaaaact gcggccaaca gtatcgagag tttccaaatg tataaatata
      241 ttacttcaca tcaatcaatt caaaattcag tgatcgtaga cattcaataa cgtaagtgta
      301 atcaacaaat tcttgctcca atctcacatt gactccatac agatccgacg ataacgactc
      361 atagattttt ttttcatctt ttgatagctg gttaagtaac gctctcttgt ttggttttga
      421 ttcgcgaacc cacttgtttc tgaaacgcat aagtgtttga taatccatca tgagcgatct
      481 gctggttgga aatatatttt taaactgatc taaaataaca aacccgtggg tatcaatatc
      541 tccccagtac catatgtttg ctcgttctaa ccattttatc tgtttaagat agctaaaacc
      601 gtatccacgt ccaaatataa caagagagtt tcgcgtcatc ggaaatgtta acgcattaat
      661 atcattttca ataacaaata cattatcagg ttctatttca agtcgatcaa gctcatcatg
      721 acgtacggaa acctctgaca gaccgtttat aaataaatca gaatcaagaa attttagtct
      781 aaccaatatc ggtttctttt taaaacccag aaaatcttca aaagattttc tttcaccact
      841 atcgtaatta tcacccggct ctatcagact taaatacaaa tcaagcagaa gtcccctgtg
      901 tttttcaata aattttgaat caatatcggg cacaggtatc tgtcttgtgt aaatagatga
      961 ctgtggattt tttaaaagcc actccagaag actaacgcat tgcttccaac caatatcagg
     1021 atcagttaac tgaagaggtt ttttaattat ccattttttt agatctggca atctatctaa
     1081 taatagtgct atgttttctt taaacaatag gagctcattt tttttagata taaactgtac
     1141 aatagtttct atatcatgga acaggagagc cgaaggaata ctatttttac caatctgcct
     1201 gtttaccttc tccttccact caatatcaaa tgaaaactct gactttttgt ctgtaaattc
     1261 agatatccat aatcggcaat ggctgtactg ttgagccatt tcagtagctg agggacctac
     1321 aagtgaaaca cgaagcggca aagaaatact gttacaatca tcaggatcaa gcaaaaacct
     1381 caaaaccgat cctctttccc aaacgcgaat caacttattt tttacatcac ttaagtcggt
     1441 ccacttttta acaggtctct ttttagggct gcttttcact tttttccttt tgatactctt
     1501 ccattgttat ttcacgcaaa accgactcac gaccttcagg attacttaca aaaccaagac
     1561 gcgaaacata gggttcaata attctgattt tttgcaacgg agttactatc aacagctgca
     1621 aatccagtct tctaaagagt tccaacgcat aacgggcaga ctcatcagaa ccacgaccaa
     1681 aggcttcatc aattacaaca aacctaaaac tgcgcgatct gacctcaccc cattcaagac
     1741 caaactgata agccagactt gccgcaagaa cggtatatgc cagtttctct ttttgtccgc
     1801 ccgactttcc acctgaatct gtataatgct catactcaga attatcctcg ttccaacgct
     1861 ctgatgctgc aaagacaaac cagttacgaa catcagtcac ctttcttgtc cagcgtttat
     1921 cttggtcact tctatcatca cgccctctaa atttttcaat aatatctttg acctttccaa
     1981 agcgcttttc actctcttgc tcgtcaaaat ttacggcaag tgctccttcg gtacaatctt
     2041 ttaaatcatg ctgaaaatct ctaatttcaa tatcactatt tgtcaaggct tccagctgaa
     2101 tataacgatc tcggttgtat tcgataccag ctaatgataa attgatcctt tcgatacgtt
     2161 ctttgatatc ttgacattcc ctgttaagct gtgattgaaa attggcaatt tcgcgtatgg
     2221 tattttcgtt aagaagctct ttgaattttt tttcaaatcg cggtagatca tccgccaaaa
     2281 gattttcaag tatacgatta tattcaggac cagcatcaat acttgcatca gtctcgacag
     2341 tctcggcggg gtactctcga caaaaatcgt gcatatcctt gataatattt tccgaaagtc
     2401 tttttatttt tctctcaata gagtcaattt ttgactgtat taactcccgc accttctgct
     2461 caagtctgtc agcagattcg attgtaattt ttttattatc tgatatttct tcaacaaatt
     2521 cttgcaactc tttgggatca acaaaaaact ccttttctgt ttcaagttga acctggcact
     2581 cctgcaagta tgacttactc tggttctgct tctcatcatt gcgcgctaaa tcactcttta
     2641 aattatcaag cttcaattct tcttcacgca tcttcttttc aagcagcccc aagtcttttt
     2701 gcaaagaggc cagtacgtcc gatgatgaag taaactcctc aagctgcttc tgataatctg
     2761 ctatttcaac aaccagtggt ttccagttaa tatcttcata atcagtataa gaatctatca
     2821 taagcattag acgcttttga ttatctatat tgttctgttg cctttttaaa tccgaaatac
     2881 ttaattcgat atcactaatc tttttttcag catcactttt agacagtcgc aacaattcaa
     2941 tcttttttaa attgttccag cccaaaatat accgtgatct gtcatgaata tcgtaacggt
     3001 catctttttc atgatgatta ccggatcttt ttatctgacc gttaatagaa agggcgtaag
     3061 catgatgtct aaactgttcg gcattttcac agcacacaaa gttgtatctt ttcaaaattt
     3121 cattctcgat ccagccgcta aaagcagatt ccgggttaac aagaatttta cttcttaatg
     3181 ataaactatc cggcgcttca ataagattag taaattcacc agcaactctg aaatatacaa
     3241 gacgaccacg caaattttcc ttatctaccc agtcacaaac cgaagaatat agctcttcag
     3301 gaacaagtag cgaaagacca aaactgtgca aaagtctttc aatagctccc tcccacaatt
     3361 tttcgggctc acaaacagct ataagttcac cagcaaaagg taatttttct tcggagacac
     3421 caagcgcggc gcaaagagca tcccgaattt gcacctgcct cctacttata ttactatgtc
     3481 ttttttcaag agattggatc tccctttcaa tctcttcttt attgtctttc agttctttaa
     3541 ggctgacttc aagctgtacc ctgttatttt gaatctcggc ttcatcattt tttaccgcag
     3601 tcagtttttc actgatatta tcttttatat tgtaaaacat tttttcagaa tcaggatagt
     3661 ccacatcaag ttctttgatc agtttctgat attccgctgc acgattcttt ttgtgatcac
     3721 gctctcttcc aagccttgca attgactgtt taatttcttc aacgcgattt ccaccttttt
     3781 cggcaatatc ctgttttaat ctgtcacgtt caagctgacg tttttcacga agactgttac
     3841 cgtttactac agatttattt aatgaatcag cttctcggca taatcgctca attcggtttt
     3901 ccaaaagagc ttttttgata tctgtaaaat agtatctaag cccagaacgt tttcttacat
     3961 gatcattttt taattgtgat tgctcattgt atattttcaa attagcaata attggattaa
     4021 gtagatctat ttgccttctg gcacgacgta cggcatcatg cgccctgtta agatcatcaa
     4081 agtgttcgat cagcatctct attcgcggag caatctcgaa aggctcaagc atatgcccac
     4141 gtacaaaatc tgtaaggttt ccaaccgact tcatggatac ggtctgatga aagagttcaa
     4201 gcgcctgctc gttagccaat ccaaaacgac gtctaaatac agcgccatac ggcggaaagg
     4261 tctcacatac ttcagcaatg ccatgttcat tttttaacct tttgcgcagt gtttttatat
     4321 cgtttccaaa gttcgaaaaa tgttctacta ttgaaagctg tctgtcggca accacataaa
     4381 accggcttgg ttggccagtg ggttcttttt gataaaagac ctgcgcgagt gtaacgctct
     4441 gcgaatagcc ctgattataa aaaacagcaa gtataaccga ataggtgcca gtcgtacgta
     4501 gcgatataga cttggttccg tatccggctt cactgcgttc atgcttgtaa tagccaagca
     4561 cataagagcg gagtgaacgc tcacggcttt cggcgccagc cgccttattg tacgctatgc
     4621 gatgtgacgg cacaagaaga gttgtaaccg catcaacaag cgtcgatttt ccagaaccga
     4681 tatcgccagt gacgagggca ttttctccgt caacagaaaa actccagacc tttttatcaa
     4741 aagtacccca gttatagagt tcaagcgtct ttaagcgaaa gcccgcccta ctgttatccg
     4801 attcaaattc aagttccgga atcattcatt cacctcttta acgccggcat atttaacata
     4861 ctcctgtaat ctggtgtcaa attccgcaag ccagctcgcg tcaacaaacc ccttgataat
     4921 tcgacacacc tctacggagg aaccaccctc cccgatagat cggacaaaac ccatggattc
     4981 aattttatta atagaggtgt caaacctttg cttaaatctg acctcgtttg taccttgcgg
     5041 aaaaaaggtt ttgatatctt caagcagatc atcacgttca attatcaatc ttctttctcc
     5101 gctggaagaa tcatgctcta caagtttttt tcttagtata gcaagaagaa gactcacact
     5161 aaaagagagc tgccttcttg gcataagccg aggtatttca tcagcgccct cttgcgcagt
     5221 ggtctgtttt aaccaagcga aaccctcatc ttcataaatt attacatcaa gccccagcac
     5281 tttaaaatag tccctgactg cagactgtcg ctttaaaagt tcatcccaca atttatcgtt
     5341 atcaaccgag taaattaccc ctttaagcaa tttaaccagc acggatgaaa actgtgtacc
     5401 attttcttct gtaatcatga cttccccctg caatacacaa cacggcaaca attcgccctg
     5461 cggatattgc cctcatcatc ttcccactct attgtttcat ttacgctctc atcaaaaatt
     5521 acgttttcat cttcggaagc gataaccaga taaacaataa gctctgccgc tccgcattgt
     5581 agtttataac gttgtgtgat ctcttgaagg ctaacctgcc ctttttccag aagaaacgta
     5641 ttgatattct tacgcaacat tactttgtcc acagttatct gatcatatag ctgagaggta
     5701 tctatgtcac cggtttctgc agaggttatt tgagaatcaa aattaatttc aatcggcgga
     5761 gaaaagagcg gacgctccat ataaagatca atatcagcgg tcattgaatc aatgctcata
     5821 aaatctccag atggcagcat atccctaacc ttaagcgagt tactacttat tgactcaagt
     5881 atctgtataa cacgacgatt ttcaagataa acctgattat caagataacg tcgcaactgc
     5941 tgcgaaagac ttgcgactct acgctgcgta tgttcacccg cctcaagcca atcgtaatgt
     6001 attctcttaa tccggttatc aggttttaac ttttgtatgg cttctattga aaaaacattt
     6061 tcaagcagcc gtgtcaactc ctcctgtgaa ttgggtgaca tcaaaaaatc ccaaaaagcg
     6121 ttaaaagtac gcccctggtc agaatcaaga ataaggtcac gatcaccaaa aatttcttct
     6181 ataagctcgc ccttgtttcc gctccaactg ttaatttttt cacgaaccgt tctatcaaga
     6241 aacctgaagt tttgctcaac ctcccgaaaa tcccccaata attctcttgc cgtactttcc
     6301 atctgtatat agctgtcgcg taaaactgca tcatcggcaa ttacgtgttc gcctctttca
     6361 agagcatcga tttctttgtt tattgcatca cgctgttttt ttagctcggc aattcttgca
     6421 tttgtgtcag tttcggcacc ataagcaatt tgtttcaaaa gatcaaaaat aatatttaac
     6481 cgggattctg taccaataaa atgttttctg gataattttc cgatccatac caacaccttt
     6541 tccgaagctg gtgataagtc ataacaggcc tcatccgatc cataacggta atacttacgc
     6601 agccaccccc tgtcatctga agaccactca tccaggtagg ccgatgcgtt tcttgagaaa
     6661 cgaggctcat cttcactatt gttcaggtag tataaaaaat catcaaggcg ggaaagcaat
     6721 tgatagtgaa atatttcatg aatattattt ttaatgaaaa catcgtgcaa aaaagcaata
     6781 acaaaggggg cattatcagc tcttaacaga cgaatagacg aatgtgtttc aaaaagtctt
     6841 tttaatgtat aataatcgaa atccatacta agtaattcta ttattaccga ataattggca
     6901 agaaattaat ctaaattctc ggattacatt gattacgtca aattttgcgg attatggata
     6961 atatatttcc gtatacggtt taattcatcg ttattgcgta ttattctatc gtaataggat
     7021 ttttgccatt gaaacgatgg gttaccggat aaatgaatca tttttgacgc ggttgtttta
     7081 aatgcgccta ttaattccga taaaatatat tccatctatt attccttttt caaaccacga
     7141 aacacccgaa acaatcgaaa cgaaaacatc tttaaacctt cttttttcgc gtttttcgcc
     7201 tgcctatcgg cagacaggtg gttcaatctt tatctttcaa tttttttata ccactatcct
     7261 cagttagcaa tttaatctgc gaaatggcaa tctggtttaa ttttaacaac cgctcatttt
     7321 gtggtaaccc ttcctgaata aaaagagcat tcaggttttc aagatttgac aaacaaacaa
     7381 gttgcgaaat ttcagcaaaa tcccgaatat ttccttttaa gtcaggattc ttatcccgcc
     7441 aatctttagc ggtcatccca aaaagagcca tattcaaaat atctgcttct gttgcataca
     7501 caagatttat ctgctctttg gataattctg gcggtatcag attttgttta atggcatcgg
     7561 tatgaatacg ataatttatt tttgtaaggt ttcgacgaat atcccaacca agttgcttga
     7621 gttcctcatc ttttaaacgc tgaaattctt taataagata caacttgaat tctacagaaa
     7681 tccaggaagc aaattcaaaa gctatatcct tatgggcgta ggttccgcca taacgaccag
     7741 actttgaaat tatacctatg gcagatgttt tttctatcca ctgttttggg gtaagcacaa
     7801 agctgtttaa tccagcctga tttctaaacc cctcgaattc gatgggttta aaatccagat
     7861 tatacaattg ctcccaaata ccaagaaact caatagttga acggttcctt agccaattct
     7921 ggataatata gtctgtcctt tctggatctt tgaatcgcgc catgtctgta aggcagatat
     7981 agtcctcttt attttgagta aaaaatgtga tctctttgtc taaaacactt atcttagcca
     8041 tacttccccc tttatctttc aaaccacgaa acacccgaaa caatcgaaac gaaaacatct
     8101 ttaccc
//
LOCUS       DAOTGZ010000123         8068 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_3064
            NODE_10757_length_8068_cov_4.624360, whole genome shotgun sequence.
ACCESSION   DAOTGZ010000123 DAOTGZ010000000
VERSION     DAOTGZ010000123.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868088
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 8068)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8068)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:22:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,785
            CDSs (total)                      :: 2,751
            Genes (coding)                    :: 2,732
            CDSs (with protein)               :: 2,732
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 12 of 19
            Pseudo Genes (internal stop)      :: 5 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8068
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10757_length_8068_cov_4.624360"
                     /isolate="AMR_MDS_3064"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(115..1614)
                     /locus_tag="P5123_08765"
     CDS             complement(115..1614)
                     /locus_tag="P5123_08765"
                     /inference="COORDINATES: protein motif:HMM:NF013739.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HRX16392.1"
                     /translation="MTKIIIIGSGIAGLASGCYAQMNGYSSTIFEQHSIPGGLCTSWY
                     RKGYTIDGCIHWLVGSSPKSGFNKYWKELHALNGVTSVYHDIFTTIETGTYKDKKTFI
                     VYADTGKLNTHMKQISPEDSTEIDTFTSLIDKFSSFEMPTDTPPELMKIGDFLKMIKK
                     IKPYTKEFRMYRKLTIADYASKFTNPHLREGLTNILNMPDFSFLGLIFMLGWQNSRKA
                     GFPVGGSLEFSKRIEKRYHSLGGTIKYKSPVQKILIKNNRAIGIELNNGEQYYADIII
                     SAADGYSTIYSMLSGKFTDKKIDRMYKTLPLFEPLFLLSIGVKRDFSSHPCMEIKHLQ
                     SPIIIEGKKHAKLGIKHYCFDPAMAPKGSSLLEIYYTSDYDYWKKLSQTPDLYNKEKN
                     KIAETIIGALESLYPGIKQDIEMTDTATPLTFERYTSNRRGTFEGWLLTPKALTLKIK
                     KTLPGLKNFYMTGQWVQPGGGIPSAAKSGRDLIAIICNKDKKVFTTTTT"
     gene            complement(1653..2339)
                     /locus_tag="P5123_08770"
     CDS             complement(1653..2339)
                     /locus_tag="P5123_08770"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRX16393.1"
                     /translation="MNIIIKNLCGTKLILLMLCIFTASSLFAEQYRYKTSVGGDVFTT
                     TINIKKLPGSEKYLLTSLDKMTGKNHQITLNSGYESIQWVIKNINEETITFIRDDNVL
                     KVSGLHAGEHIKKSLIIDEAPWFASLDFGIQAFSKSGKAEINFWVIDPSNLKPHLMTL
                     TRMNEETVTHAGKKTQTVKIRITASGVPALFFNMNYWTRTTDGLPVRSETKLGGPLSP
                     TILNELIIND"
     gene            complement(2479..3300)
                     /locus_tag="P5123_08775"
     CDS             complement(2479..3300)
                     /locus_tag="P5123_08775"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRX16394.1"
                     /translation="MKAFLSKYPLATHELIHFPLMYPDTLKKYRIRNIPYASLDLLYT
                     YGQACGVSKGALRTTLSRMKKENSIVPVTIDGKPRYRVAPLQIEVMTNMQKKKKDKSN
                     WFTVAIFSFEKSQEKQRSITRSLLLYCGFVRFAQNAYINIRGKDDQLRKSLYDAGVGD
                     NVYLFNVSSIEDSDLQKLLSCWKIPERIELLKTFRNDAEQLIENNSRTDADLFFSIGL
                     AWVAYIIHISSTEPPLPLNVLPKDYPYNKLYNYMVKKSIKYSKKMIQYWRSSNAI"
     gene            3534..4487
                     /locus_tag="P5123_08780"
     CDS             3534..4487
                     /locus_tag="P5123_08780"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRX16395.1"
                     /translation="MSKLFRICKYKIICLIKHLSELDNVLRAFIVLSTSLLLLIVNDS
                     LLKYNNILPGAITGKLSDFAFVLIYPALVIWVLRIKRPVAAIIISIATVLPFVLININ
                     PAFSNYIEKIIVIGTYKIRLWPDPTDCLALILLPVSCNMGFQLNDRAIKSTAILKTVF
                     VFICVLALINTSKADSHSPIKMPKKEFRMISMEIRPPKVILKRGKIISYKRYLFVCEP
                     NAGFHVIEKMDDGSLKPLSFVFVLGNSDIVIKDDYIIANNLIDLLIFKLLPKGEISFV
                     RRVTDAIVYDPYSLLPPKAYIKYRSLETDPMKIEEVVTGWK"
     gene            4574..5452
                     /locus_tag="P5123_08785"
     CDS             4574..5452
                     /locus_tag="P5123_08785"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRX16396.1"
                     /translation="MAQMIIDDDILYYLASGELHVFDITNPSELQSKGRLALNPQIDR
                     ENNYDTLSIYEDLLLVGMNDFIIVLEKSDSNTMPVVIDMIRHDIARDPVIGYNGYLYS
                     TIRTENGASRSQDMLTVYKLDVDKLTGKTRIFEVTRRLLFNPHGLVFYNDLLLIADGK
                     WGLKGFTVVDGVPDDQVFIDKEINAYDLISAEGELFVSSEKKIIRGDLGKNKIDVYGE
                     VEVKGDEYVNGLKQISNNKYKAKILFSGFIDVLKKIGLLLLIVLGVIFSLSLGLLVVS
                     SPFFLIWFLYKRYILK"
     gene            complement(5506..7050)
                     /locus_tag="P5123_08790"
     CDS             complement(5506..7050)
                     /locus_tag="P5123_08790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012500197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008134 - transcription factor binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HRX16397.1"
                     /translation="MKDNDYEAGNIINLLYNISNSLSTNNDIKDVIKPVLNIMLQNLQ
                     VIRGMITIFNRQSGEIFIEDGFGFSDEAQTRSRYTPGEGVTGRVVESGLPIVIENIEK
                     SDILANKTGFKNNYHDNQISFICVPIRSTNHTTGTISAYKILKDSGQLETDTKLLSIV
                     ATMISRVIRLHQNVHEENMLLLAENRRLYEALSEKKSHSKIIGNSNAIRMVLEMIGKI
                     VNTSTTVLILGESGVGKERVAETIHFNSSRKDKPFIKFNCGALPENLIESELFGYEKG
                     AFTGATTSRIGKFQQADKGTLFLDEVGELSLNAQTKLLRVIQEKELERIGGTQTHTVN
                     IRIIAATNRDLSDRVNQGLFREDLFYRLNVFPITVPPLRERKTDIPLLTDFFIKSLSK
                     KLNKDKVTISRAAMDLMVNYSWPGNVRELENCIERALILCDNSTIRIHNLPLNIQDSG
                     THNNHFSGPLQNQIDNIEYEIIIQELAQNHGNVSVTAQKLGLTVRKMGLRIKKHGINP
                     NRFKKR"
     gene            complement(7040..>8068)
                     /locus_tag="P5123_08795"
     CDS             complement(7040..>8068)
                     /locus_tag="P5123_08795"
                     /inference="COORDINATES: protein motif:HMM:NF012885.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRX16398.1"
                     /translation="DFINKLIPIKPVNTKISVWCRNNISDINHALDTDAEIIHISIFC
                     SEILQKIFNKNITKQIAELKQNIQPIIDAGRKVSVGFQDVFRTPDINIIKALNAISDA
                     NIFRIRLADSCGCALPDDCSRLISKVKKIWYKKIDFHAHNDCGLAAANSVSALSSGAD
                     SLSVTVNGIGERAGNTSLSDIASIALITNSIKTGLNYQNIKALSNAVSRFSHFNVSPF
                     APLIGNHIFEHKAGIHVSALLNNPNSYQPYPDAINPATCSGSLDNNVSNNINAPQTNK
                     FLFGQYSSTGSISGFMKHYGHNLSQQKAGLLKQKIDEHATKEKTDIDGKTLISIYNQC
                     IAGGSDER"
BASE COUNT         2334 a         1342 c         1529 g         2863 t
ORIGIN      
        1 gcgttcagct gatctgtact aatcggtcgt gaggcttgac catattatta ttaccccgat
       61 tcacgtatgt ggatcggggt attttatatt taaaggattt gaacaagtta ttgattaggt
      121 tgttgttgtt gtaaaaacct ttttatcctt attacaaata attgctatta aatctcttcc
      181 tgattttgct gccgatggta taccgcctcc tggctgtacc cattgaccag tcatataaaa
      241 gtttttaagt cccggaagtg ttttcttgat ttttagggtc agggcttttg gtgtaagcag
      301 ccaaccttca aaagttcccc ttctgtttga ggtgtaccgt tcaaaggtta gaggtgttgc
      361 agtgtctgtc atttcgatat cttgtttaat tcctggatac agactttcta aagcacctat
      421 tattgtttct gcaattttgt tcttttcttt gttgtacaaa tcaggagttt gtgatagttt
      481 tttccagtaa tcatagtcag atgtgtaata tatctcaagt agagatgacc ctttaggggc
      541 cattgcagga tcaaaacagt agtgtttaat acctaatttg gcgtgttttt tcccttcgat
      601 aatgattggt gactgcaggt gtttgatctc catgcacgga tgtgaagaaa aatccctttt
      661 aactcctatg gaaagaagaa atagtggttc aaagagagga agtgttttgt acatcctgtc
      721 aatttttttg tcggtaaatt ttccggagag cattgagtat attgtggaat aaccatcagc
      781 agcagagatg attatgtccg cgtaatattg ttccccatta ttcaattcta taccaatagc
      841 tctgttgttt ttaataagaa ttttttgtac tgggcttttg tattttattg tgccaccgag
      901 agaatgatat cttttttcga tacgcttact gaactccaag gatcctccga caggaaaacc
      961 ggctttacgg gagttctgcc atccaagcat aaagataaga ccgagaaatg aaaaatcagg
     1021 catattaaga atattcgtta agccttcacg aagatgggga tttgtaaatt ttgatgcgta
     1081 atcggctatc gtcagttttc tgtacatacg aaactctttc gtatatggtt taattttttt
     1141 tatcatttta agaaagtctc caattttcat caactcgggc ggtgtgtccg tgggcatctc
     1201 aaaagatgaa aatttatcta taagtgaagt gaatgtgtct atttctgttg aatcctccgg
     1261 actgatctgc ttcatatgtg tattgagttt accggtgtcg gcataaacta taaatgtttt
     1321 tttatcttta taagtaccgg tttcgattgt tgtaaatata tcatgataga cactagtaac
     1381 gccgttaagt gcgtgaagct ccttccagta tttgttaaaa ccacttttag gagaacttcc
     1441 tacaagccaa tgaatgcaac catcgatcgt gtaaccctta cgataccagg atgtacataa
     1501 tccgccaggt attgaatgtt gctcaaaaat agttgagctg taaccattca tttgtgcata
     1561 gcaaccactc gcaagcccgg ctatccctga gcctataatt ataatcttag tcataattcc
     1621 tccggtattt tttgatatag tttattataa aatcagtcgt taataattaa ttcgttgagt
     1681 atagtaggag acaagggtcc tcccagcttt gtttcagaac gtactggcaa tccatctgtg
     1741 gtacgagtcc agtagttcat attgaaaaaa agtgctggca cccctgatgc agttatccgt
     1801 attttaacag tttgcgtttt cttgccagcg tgtgttactg tttcttcgtt catgcgagtg
     1861 agtgtcatca gatgtggttt cagattactt ggatcaataa cccaaaagtt aatttcagct
     1921 tttcctgatt tggaaaaggc ctgaattcca aaatcaagag aggcaaacca gggagcctca
     1981 tcaattatta agcttttttt tatgtgttct cccgcgtgaa gaccactcac ctttaaaaca
     2041 ttatcgtccc ttataaatgt gatcgtttcc tcattaatat ttttgataac ccactgtatg
     2101 ctttcgtagc cggagttgag agtaatttgg tgattttttc cggtcatttt gtctaaactt
     2161 gttagcaaat atttttcgga ccccggaagt ttttttatgt ttattgttgt agtaaaaaca
     2221 tctccaccaa ccgaggtttt gtaacggtat tgttcggcaa agagcgaaga tgctgtaaag
     2281 atacaaagca tgagtaaaat gagttttgtt ccgcataaat tttttatgat tatgttcata
     2341 ttgttctcct ataatgatat tagggagctt cgaaaaaata ccatatttaa gaaggtcaca
     2401 cagaaaaagt gcctacttaa aataattttt cgcaagttcc ttgaaaaagc taataattca
     2461 cgattcccat tatatagttt aaatcgcatt tgatgatcgc cagtattgga tcattttttt
     2521 gctgtatttg atgctttttt ttaccatata attgtataac ttattgtatg ggtaatcctt
     2581 aggcagtaca tttaagggga gaggaggttc cgtagagcta atatgaatta tatatgcaac
     2641 ccacgctagt ccaatactaa aaaaaagatc ggcatcggtt cggctattat tctcgattag
     2701 ttgttctgca tcatttctaa atgttttaag aagctctatt cgctcgggaa tcttccagca
     2761 gctaagtaat ttctgaagat cggagtcttc aatgctgctg acattgaaga gatagacatt
     2821 gtctccaact ccggcatcgt aaagtgattt tctgagctga tcatcctttc ccctaatatt
     2881 gatataggca ttttgcgcga atcgcacaaa tccgcagtag agcaaaaggg agcgtgttat
     2941 agaacgctgt ttctcttggc ttttctcaaa cgaaaagatt gcaacagtaa accagttgct
     3001 tttatccttt tttttcttct gcatatttgt catcacttca atttgcagag gggcaacccg
     3061 gtatcgcggt ttaccgtcta ttgtaactgg tacaatgcta ttttcttttt tcatacgtga
     3121 aagcgtggtg cgcaatgctc cctttgatac tccgcaagcc tgaccatagg tgtataataa
     3181 atcgagagag gcataaggaa tattacgaat tctgtacttt tttaaagtat cagggtacat
     3241 taatggaaaa tgaataagct catgtgttgc aagtgggtat ttagataaaa acgctttcat
     3301 agttgttaca aaaccccaaa taagtatttc tagtatagta actacagaac tacgaagaga
     3361 aaaatgtcaa cgaattattt atgtttagtc tttcgagggt aattaaagaa gtgtaaagtg
     3421 aataattggt tgacattata taaatgtagt tttataataa tatcgtttta tttgtatttt
     3481 tttaaccatg tatatttatt aagcactgtg gtcaattttg aagaggtcta tttttgagta
     3541 aattatttag aatttgcaaa tataaaataa tttgcttaat aaagcatttg tctgaattgg
     3601 ataatgtgtt aagagccttt attgttcttt ccacttcttt actgcttctt attgttaatg
     3661 acagtttatt aaaatataat aatatactcc ccggagcgat tactggcaag ttgagtgatt
     3721 ttgcttttgt tttgatttat ccggctcttg ttatatgggt gctaagaata aaacgcccgg
     3781 tagcagcaat cattatctca atagcgaccg tattgccgtt tgtattgatc aatataaatc
     3841 ctgctttctc caattatata gaaaaaatta tcgttatcgg tacttataaa atccgattat
     3901 ggcccgatcc aactgattgt ttggctctta tactgctccc ggtctcatgt aatatgggat
     3961 ttcagttaaa cgatagagct attaaatcga ctgctattct aaaaacagta tttgtcttta
     4021 tctgtgtcct ggctcttata aatacttcaa aagccgattc gcatagtcca atcaagatgc
     4081 caaaaaaaga gttccgcatg atatctatgg aaatacgtcc accaaaagtg atattaaaga
     4141 gaggaaaaat tatcagttat aaaaggtatc tgtttgtttg tgaaccaaat gctggttttc
     4201 atgttattga aaaaatggat gatggctcgt taaaaccttt gtcatttgtt tttgtgttgg
     4261 ggaacagtga tatagtaatt aaagacgatt atatcattgc aaataattta attgatcttt
     4321 taatttttaa acttttaccg aaaggagaga ttagtttcgt cagacgggta acagatgcaa
     4381 ttgtttacga cccatactca ttgctgcctc caaaggcata cataaaatac cgcagtctgg
     4441 agacagatcc aatgaaaatt gaagaggtcg taacaggatg gaaataatta aaacaatatg
     4501 tatagcaatc atattctgtt cgttcgtatc ttgcggtaaa acagtaagta cttcatcttc
     4561 tgtctcagga tccatggctc agatgattat agatgatgat attctctatt atctggcttc
     4621 aggtgaactg catgtttttg atataacaaa tccatctgag ttgcaaagta aagggcgatt
     4681 agctttgaat cctcaaattg atagagaaaa taattatgat actctgagta tttatgaaga
     4741 tttactactg gttgggatga atgattttat aatagttttg gaaaaaagcg attcaaatac
     4801 tatgccagtt gttatagata tgatccgaca cgatattgca cgagatccag taattggcta
     4861 taatggttac ctttattcta caatacgaac ggaaaatggt gcttcacgta gtcaagacat
     4921 gttgactgta tataagcttg atgttgacaa gttgacaggt aaaactcgta tttttgaagt
     4981 tacaagaaga ttattattta atcctcatgg tttagttttt tataatgatc tgttattgat
     5041 tgcggatgga aagtggggac tcaagggttt tacagttgtt gatggtgttc ctgatgatca
     5101 ggtttttatt gataaagaaa taaatgctta tgacttaata agtgctgaag gtgaactctt
     5161 tgtgtcatct gaaaaaaaaa ttatcagagg agatcttgga aaaaataaaa ttgatgtata
     5221 tggcgaggtt gaggtaaagg gtgatgaata tgttaatggt ttgaagcaaa taagcaataa
     5281 taaatataaa gcaaaaatat tattttctgg atttattgat gttctgaaaa aaattggatt
     5341 actattgttg attgttttag gtgttatatt ttcattatca ttaggactat tggttgtttc
     5401 ttctcctttt ttcttgattt ggtttttata caagagatat atattaaaat gaaactgtat
     5461 ttaatattta taaataatat acaaatataa taagcaaata attaactatc tcttcttaaa
     5521 ccggtttgga ttaataccgt gtttttttat tcgcagaccc atcttgcgta cggtgagtcc
     5581 cagtttttgt gctgtgactg aaacgtttcc atggttttgg gcgagttcct gaattattat
     5641 ttcgtactcg atattgtcta tctggttttg taaaggacca ctgaaatgat tattatgagt
     5701 acccgaatcc tgtatgttta acggaagatt atgtatacgg atagtgctgt tatcgcatag
     5761 aattaacgct ctttcgatac agttttccaa ttcgcggacg tttccaggcc acgagtagtt
     5821 aaccataaga tccatagcgg ctcttgaaat tgttacttta tccttattca gttttttact
     5881 cagtgattta ataaaaaaat ctgttagtaa gggaatatct gttttacgtt cccgtagagg
     5941 agggacggtt ataggaaaaa cattaagtct ataaaacaga tcttctctaa atagtccctg
     6001 attcacacgg tctgacaggt cgcgatttgt tgcggcaatt attctaatgt ttaccgtgtg
     6061 ggtttgtgtc cctccaattc gctccagctc cttttcctga attactctta gcaattttgt
     6121 ctgtgcatta aggcttagct cgccaacctc gtcaagaaag agcgtcccct tatcggcttg
     6181 ttgaaatttg cctattcgag aagtcgttgc accagtgaaa gcccctttct catagccaaa
     6241 aagttcgctt tctattaagt tttcaggtaa ggctccgcag ttaaatttta taaaaggttt
     6301 atcctttctt gaagaattaa aatggatagt ctcggcgaca cgttccttgc caacaccgct
     6361 ttctccgagg atgagtacgg ttgttgatgt attgacaatt tttccaatca tttcaagtac
     6421 cattctgatt gcattagagt taccaataat ctttgaatgg cttttctttt cggagagtgc
     6481 ttcgtacaat cttctgtttt cggcaagcaa aagcatattt tcttcatgta cattttgatg
     6541 caatctgatt accctagata tcattgttgc aactatggaa agaagttttg tatctgtttc
     6601 tagttgacca ctgtctttga gaattttata agcactaatg gttcctgttg tgtgatttgt
     6661 ggagcggatt ggtacacaaa taaaacttat ctggttatca tgataattat tcttgaaacc
     6721 agttttattg gccaggatat cacttttctc tatattttca atcacgattg gcaaaccgct
     6781 ttcgactact cttccagtaa caccttcgcc tggggtataa cgacttcttg tctgagcttc
     6841 atcactaaat ccgaatccgt cctcaataaa tatttctcca gattgtcggt taaaaattgt
     6901 aatcattccc cttataacct ggaggttttg aagcataatg tttaagactg gtttgatgac
     6961 atctttaata tcgttatttg tagagagcga gttgctgata ttgtaaagaa gatttattat
     7021 gttcccagct tcataatcat tatctttcat ctgatcctcc agctatacac tgattgtaaa
     7081 ttgaaataag ggtctttccg tctatatccg ttttttcttt tgttgcgtgc tcatcaattt
     7141 tttgttttag caagcctgct ttttgttgag acaaattgtg accgtaatgc ttcataaaac
     7201 cgctaataga tcccgtgctg ctgtactggc caaaaagaaa cttgtttgtc tgcggtgcat
     7261 tgatgttatt gctgacgtta ttatccagag atccactaca agtggcagga tttattgcat
     7321 ccgggtaggg ttgataactg ttgggattgt tcaaaagagc tgaaacatgg atccctgcct
     7381 tatgttcaaa aatatggttt ccaattaatg gcgcgaaagg ggaaacattg aaatgactga
     7441 atctagatac ggcattagat aatgccttaa tattttggta attcagtccg gttttaatgg
     7501 agttggtaat taatgcaata gacgcgatat ctgataatga agtattccca gcacgttcac
     7561 caattccatt cacggttact gataatgagt ctgcaccact tgataaagct gaaactgaat
     7621 tagccgcggc taaaccgcaa tcgttatgtg cgtgaaaatc aattttttta taccatattt
     7681 tttttacttt ggatataagt ctagaacaat cgtcgggaag tgcgcaccca catgaatctg
     7741 ctagtcgtat tctaaaaatg tttgcatctg atatggcatt aagtgcttta ataatattta
     7801 tatctggtgt tctaaacaca tcctggaatc caacactgac ctttcttcca gcatcaatta
     7861 taggttggat gttttgtttt aattctgcaa tttgtttcgt gatgtttttg ttaaatattt
     7921 tttgaagtat ttcagaacaa aaaatagaga tatgaataat ttctgcatct gtatctaatg
     7981 catgattaat atctgatatg ttattgcggc accatacaga tatttttgta ttaaccggtt
     8041 ttattggtat tagcttatta ataaaatc
//
LOCUS       DAOTGZ010000124         8039 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_3064
            NODE_10802_length_8039_cov_6.939254, whole genome shotgun sequence.
ACCESSION   DAOTGZ010000124 DAOTGZ010000000
VERSION     DAOTGZ010000124.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868088
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 8039)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8039)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:22:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,785
            CDSs (total)                      :: 2,751
            Genes (coding)                    :: 2,732
            CDSs (with protein)               :: 2,732
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 12 of 19
            Pseudo Genes (internal stop)      :: 5 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8039
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10802_length_8039_cov_6.939254"
                     /isolate="AMR_MDS_3064"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(133..405)
                     /locus_tag="P5123_08800"
     CDS             complement(133..405)
                     /locus_tag="P5123_08800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017850895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BrnA antitoxin family protein"
                     /protein_id="HRX16399.1"
                     /translation="MKTLKKIPKFMNETEEFEFWANNDSTEFLNLKEAKKLHFKNLKP
                     STKSISIRLPESLLERIKIIANKNDVPYQSLIKIYLSERVKEETVK"
     gene            complement(368..694)
                     /locus_tag="P5123_08805"
     CDS             complement(368..694)
                     /locus_tag="P5123_08805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002977461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BrnT family toxin"
                     /protein_id="HRX16400.1"
                     /translation="MNFEFNIDDIIGFDWDEGNSKKNWERHQVSIGESEEVFFNEPFY
                     IFSDEKHSNSESRYYVLGETNKSRYLFIVFTIRKCKIRVISSRDMNKKEKTIYENLKK
                     NTEIHE"
     gene            1107..3152
                     /locus_tag="P5123_08810"
     CDS             1107..3152
                     /locus_tag="P5123_08810"
                     /EC_number="3.6.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013628456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium-translocating pyrophosphatase"
                     /protein_id="HRX16401.1"
                     /translation="MDSLLLYFPLGAGVLSLLFALYRVLWVGKQDPGSETLIEISHAI
                     RSGAFAFLRREYKVLAIIVICVAALLVIGEHGATRLVALSFVVGAFLSGTAGLIGMHA
                     ATLANSRTAQSAKTGLASALSVAFAGGSVMGLSVVGLGIIGLSGLLLIYTAIFGFEIM
                     TLKETVLPIINGYSLGASSVALFARVGGGIFTKAADVGADLVGKVEAGIPEDDPRNPA
                     VIADNVGDNVGDVAGLGSDLCESYIGSIIGAMVLGGAALSVRLVVLPLVIAAIGIIMS
                     IIGMFFVRTREGGNPQNALNFGTIGASVLMLVFTFPAVWYLAPRTFSYENISYSSIGI
                     IVAIITGLICGVLIGMVTEYYTADNRKPVINIARASTTGAATNILGGIQVGMVATGIP
                     VILLAAAIMVAYHAAGLYGIAIASMGMLATTGIQLAVDAYGPIADNAGGIAEMAAMDP
                     EVRERTDKLDAVGNTTAAIGKGFAIGSATLTALALFVAYKETVGLTMIDATRPRVLVG
                     LLMGGMLPYVFSSFLIGSVGKAAFSMIEEVRRQFRTIEGLLAGKAKPDYVKCVDIATK
                     AAIREMIIPGVLAVLIPVVVGFFGGAEMLGGVLVGVMISGVMLALFMANSGGAWDNAK
                     KHIEGGAFGGKGSDAHKASVIGDTVGDPFKDTAGPSMDILIKLMSVVSLVIAPIISR"
     gene            3163..3933
                     /locus_tag="P5123_08815"
     CDS             3163..3933
                     /locus_tag="P5123_08815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020612883.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRX16402.1"
                     /translation="MKIYYYLTVFPTEGLIASQLGPEDFGSYMATGSKKGTAEHLIFI
                     EVSGEFGSYFDWDYAIQNCVPHSDGRPKHSKYLAVYRALENTPLDKLGAMYLTTRDGR
                     SVKLEKSVYKEPENPAEFHLYQELCPVYPLVVSKLNAGNFAKHLTSDSNKVFVPQIVF
                     ADLKFFNVDDPEHTGNIGTLYSRNIQHLKGCIAQLRSSNDKQNKTLDRTHVESFGFQH
                     IGKAIYVANSKETIAYNMLTMDELQRDHFPWAKSANIL"
     gene            complement(4040..5188)
                     /locus_tag="P5123_08820"
     CDS             complement(4040..5188)
                     /locus_tag="P5123_08820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009275000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HRX16403.1"
                     /translation="MQRRIAALPERIRLFTQEQSLPQSLLLTGPRGVGKTTCLLYHAA
                     QNSDKTLYFSADNPLLISESLFQLCSSIFMTGYKGVIIDEVHFAKDWSLHLKALFDDF
                     PDRKIWVSDSSSLVLRNSRGDLSRRFVSVAMPFLSFREFLHLETGAKYPKHDAFNLDK
                     EIPLAPTSEILDLFRTYREYGTRPFYKDGNFQDRMLAVLDKTLYYDIPFFLPQITDGN
                     LRLMKAIVGTLSQNTIPRIQVRSLCSDWGIGAEKLYQLLEVMESVGIIRIIRKENDTK
                     ARSTGAKLFFSDPAMYAILSGKQGTAREALVATLCALSGWQVWASKKEEHGDFILTSA
                     NQLYTKKRILIEVGGKNKSVKKADYVIRDDIDYPAGNAIPAWLLGMGF"
     gene            complement(5408..5908)
                     /locus_tag="P5123_08825"
     CDS             complement(5408..5908)
                     /locus_tag="P5123_08825"
                     /inference="COORDINATES: protein motif:HMM:NF024698.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HRX16404.1"
                     /translation="MKILETGRLFLEEIGEDRFEDLANLLANEKVHTFFPKTLNRKES
                     RDYLEIIRKRQKNDGISFWAVIRKDDLKFLGICGLLKQNIDDTDEIEVGYRIDDLFWG
                     QGYGTEAAKGCLQYAKEKLKIPSVISLILPENTQSVRVAEKNGLILQKKSMFHGQMHN
                     VYRITF"
     gene            6164..6757
                     /locus_tag="P5123_08830"
     CDS             6164..6757
                     /locus_tag="P5123_08830"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRX16405.1"
                     /translation="MKLMLNDEIFTKRYRKFLFQFPLLLIIVSVLGAFIYHSQTIFFE
                     KGDISSIINIDLLTSSFFHGSIVAVVIYFIKSRKLEKIFKEKPSDVAENKIDTFLPCL
                     HGVWPYQYQDAFLTTEGTALRLYVKKVGGFVMVCEWNNLSMLKIEAVREPLNPFLPVL
                     FNTSKSIRISDGKKSKKILFAEPEQTAEELSRLVKSM"
     gene            7000..7596
                     /locus_tag="P5123_08835"
     CDS             7000..7596
                     /locus_tag="P5123_08835"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HRX16406.1"
                     /translation="MALYKNKYRVESVRLKGWDYSSPGHYFITICTKNRECLFGNIVQ
                     NKIVLSDMGKIMERYWREIPDHFNGVKLDEYVIMPNHIHGIISIMVETPNLGVSTGTD
                     PETGTDPETGTDPETGTDPDKKNTPNIGIIINQYKRICTIRIRESYPGIIIWQTRYHD
                     HIIRDENELYRIRQYIIKNPANWEKDDKYKKEPCRTSR"
BASE COUNT         2339 a         1540 c         1634 g         2526 t
ORIGIN      
        1 ttgaggcaaa tcgcaatcaa atttcactaa ataaatatgt tgaaaacgca ataatagatg
       61 aattgagcaa atctcaattt atataagaaa acagagaaaa atgaatcaag gtctgtacgg
      121 ctgcttcttc aattatttta cagtctcctc cttcacacgt tctgatagat aaatttttat
      181 taacgattga tagggaacat cgtttttgtt cgcgattatc ttaattcttt caagcagtga
      241 ttccggcaaa cgaatggaga tgctttttgt tgatggctta aggtttttga aatgcagttt
      301 tttcgcttcc ttaagattca aaaattccgt tgagtcatta ttggcccaaa attcaaactc
      361 ttctgtttca ttcatgaatt tcggtatttt ttttaaggtt ttcataaata gttttctcct
      421 tcttgttcat atctctggat gatattaccc gaatcttgca ttttcgaatt gtaaaaacaa
      481 tgaacaaata ccttgatttg tttgtttccc ccagcacata atatctgctt tcagaatttg
      541 aatgtttttc atcactaaaa atatagaacg gttcattaaa aaagacctct tcagattctc
      601 caattgatac ttgatgtcgc tcccaattct ttttactgtt accctcgtcc cagtcaaaac
      661 cgattatatc atctatatta aattcaaaat tcatgtatat taagataata tacaaatagc
      721 taaagtcaag gattattaat tagcaattat ttgtaatgtt attcagcaat atagcaggac
      781 cctctattaa tgaacttttc aaccaggctg cgagcagcct atcaccattt tcttttcaaa
      841 atggtgctgg atgaccagag taggaatctg caaaaaattc caacagaata gatatttaaa
      901 agttgatact ttcagaaaaa gataatttta cgaatttccc ttgtgttaat ttttctttaa
      961 ttaactgtac ataaatttta aagtgtgaaa ttatcttgac tgatagcgag tataatttaa
     1021 tcagcataca cagttaattt taattgttat gaatagttct tttatatcaa aaaattacaa
     1081 ataaacataa agatgaggag taaaaaatgg attctctctt attatatttt cccctgggag
     1141 cgggggttct ttccctgttg ttcgctttat acagggttct ctgggttggc aaacaggacc
     1201 cgggtagtga aacacttata gaaataagtc atgcaattag atcaggcgct tttgcttttc
     1261 ttaggcgtga atacaaggtt ttggctatta ttgtaatctg tgtcgcggcc ttacttgtta
     1321 tcggtgagca tggagcaaca cggcttgtgg cgctctcttt tgttgtagga gcctttcttt
     1381 caggaacagc cgggctaatc ggtatgcacg cggcaaccct tgccaacagc agaacagctc
     1441 aaagtgcaaa aactggcctc gcgagtgccc tttctgttgc atttgccggt ggaagtgtta
     1501 tgggcttaag tgttgtaggt ctgggtatta ttgggctttc gggtcttcta cttatttata
     1561 ccgctatttt tggtttcgag attatgacct taaaagagac agttttacct attattaacg
     1621 gatattcact tggagcaagt tctgtggctc tctttgcccg cgtaggcggc ggaattttca
     1681 caaaggctgc cgatgttggc gccgaccttg ttggaaaggt cgaagccggt attcctgaag
     1741 atgatccccg taacccggct gttatagcgg acaatgttgg tgataacgtt ggcgatgttg
     1801 cgggacttgg ttccgaccta tgtgaatcgt atatcggttc aattatcggt gcaatggttc
     1861 ttggtggggc ggctctctcg gttcgtcttg tggttttgcc tcttgtaatc gccgctattg
     1921 gtattataat gtcaattatt ggtatgtttt ttgtaagaac aagagagggt ggaaatccac
     1981 aaaatgccct aaacttcggt acgatcggag ccagtgttct gatgcttgtt tttacctttc
     2041 cggcagtctg gtatcttgct ccgcgtacat tttcatacga aaatatctct tattcaagta
     2101 ttggaataat cgtcgcgatt ataaccggat tgatctgtgg tgtgctaatc ggtatggtta
     2161 ccgaatacta cactgccgat aatcgcaaac cggtaataaa tatcgcgcgt gccagcacaa
     2221 ccggtgctgc aacaaatatt cttggcggaa tacaggtcgg gatggttgct acaggaatac
     2281 ctgtaatact tcttgcggcg gctataatgg ttgcctacca cgcggcaggt ctttatggta
     2341 tagctatcgc ctctatggga atgcttgcca caaccggaat tcaacttgcc gtcgatgcct
     2401 acggaccgat cgccgataac gccggcggta ttgccgagat ggctgcaatg gatccggaag
     2461 ttcgagagcg cacagataaa ctggatgctg ttggaaacac taccgcagct attggtaaag
     2521 gtttcgcgat cggctcggca acattaactg cactggctct ttttgttgca tataaagaga
     2581 ctgttggcct taccatgatt gacgcgacca ggccaagggt acttgtgggt cttcttatgg
     2641 gcggtatgct tccttatgtg ttctcttcgt ttttaatcgg atcggtcggt aaagccgcgt
     2701 ttagcatgat cgaagaggtg cgccgccagt tcagaacaat cgaaggtttg cttgccggca
     2761 aggcaaagcc tgattatgtt aaatgtgtag atatcgcgac caaggctgcc atccgtgaga
     2821 tgattattcc gggtgttctt gctgtactta ttcccgttgt tgtcggcttt tttggtggtg
     2881 ccgagatgct tggtggcgtt cttgtaggag taatgatttc gggtgttatg cttgctcttt
     2941 ttatggctaa ttctggcgga gcatgggaca acgccaagaa gcatattgaa ggcggagctt
     3001 tcggtggaaa aggatctgat gctcacaagg cttcggtaat cggtgacaca gtcggagatc
     3061 cctttaaaga tacagccggt ccttcaatgg acatacttat caaactgatg agtgtggtat
     3121 ctctggttat tgcccccatc atttcacgat aaggagaagg atatgaaaat ttactactac
     3181 ctcactgttt tcccgacaga gggacttata gcttcacagc ttggacccga agattttggc
     3241 tcttacatgg caacaggatc aaagaaggga acagcagagc atctgatttt tatagaagta
     3301 agcggcgaat ttggttcata ctttgactgg gactacgcta ttcaaaactg tgtgccgcat
     3361 tcagacggaa gaccaaaaca ttcgaaatat ctggctgttt accgcgcttt ggaaaacacg
     3421 cctcttgaca agttgggcgc catgtatctt acaacccgtg atggcagaag tgttaagctt
     3481 gaaaagagtg tctacaagga accggaaaac cctgcagaat ttcatcttta tcaagagttg
     3541 tgcccggttt atcctctggt tgtaagtaaa ttaaacgcgg gtaatttcgc taagcattta
     3601 acctctgatt ctaacaaggt gtttgttccg cagatcgtct ttgccgacct taaatttttt
     3661 aatgtagatg atccagaaca taccggaaat attggaacat tgtacagccg aaacattcag
     3721 caccttaaag gttgcattgc ccagttaaga tcatcaaatg ataaacagaa taaaacgctg
     3781 gatcgtactc atgtcgagtc gttcggattt cagcatattg gcaaggcaat atatgttgct
     3841 aactcgaaag agacaatcgc gtacaacatg ctaaccatgg atgagcttca gagggatcac
     3901 tttccttggg caaaatcggc caatattctg tagcttgtac ttaatacttg aatctaggat
     3961 tttatacaaa aaggctgctc gattacgggc agcccttttt gcagcttata attaatgcac
     4021 aggtataacg ggagttagtt taaaacccca ttccaagcaa ccatgcaggt attgcgttac
     4081 ccgccgggta atcgatatcg tcgcggataa cataatcggc cttcttaaca gatttgtttt
     4141 tcccaccaac ttcaattaat attcgttttt ttgtataaag ttgatttgca gatgttaaaa
     4201 taaaatcacc atgttcctct ttttttgaag cccagacctg ccagccggat aacgcgcata
     4261 gagttgccac aagagcttct cttgctgtgc cctgctttcc ggaaaggatg gcatacattg
     4321 ccggatcact aaaaaaaagc ttggcaccgg ttgaacgggc cttggtatcg ttctctttgc
     4381 gaatgatccg tattatacca acagattcca ttacttcaag aagttgataa agtttttctg
     4441 cgcctattcc ccaatctgag cataaagaac gaacttgaat tcgaggtatt gtattttgcg
     4501 acaaagtgcc aacaatcgcc ttcataagtc ttaaatttcc atcagtaatc tgaggtagaa
     4561 aaaatggaat atcataataa agggttttat caagaacagc gagcatccga tcctggaaat
     4621 ttccatcttt ataaaaggga cgcgtaccat attctcgata agttctaaac agatcaagta
     4681 tttcagatgt cggagccaga ggaatttctt tatctaaatt gaaagcatcg tgtttcggat
     4741 attttgcgcc tgtctctaga tgtaaaaatt cccgaaaaga gagaaaaggc attgctacgg
     4801 aaacaaaacg acgtgaaaga tctccccggc tgtttcgcaa aactagtgat gatgaatcac
     4861 tcacccatat tttacgatca ggaaagtcgt caaataatgc ttttaaatgc aagctccaat
     4921 cttttgcaaa atgaacctca tcaatgatta cgcctttgta acctgtcata aagatagagg
     4981 agcacagttg aaataaggat tcagatatta ataaagggtt gtctgcagag aagtagagag
     5041 tcttgtcgct attttgcgca gcgtggtaaa gaagacatgt tgttttgcct acaccacgag
     5101 gtcctgtcaa tagaagtgac tgtggcaagg actgctcctg cgtgaataag cgtattcttt
     5161 caggaagagc tgcaattcgg cgctgcatgg tgatttcgag ttgagttatg atttcattgt
     5221 tcataataca aatatatagt aaatatgttc ctgtgtaaag aacaaaaacg gtaaaatgtt
     5281 tctgttgtag aaacatttta ccgttttttt atgaaattac ctcttatttt ctgtacttgc
     5341 agtgattttg tttaaatagt gcagtcgagc cggtcattat gcattgctat ctgaaagaat
     5401 tgaaacccta aaaagtaatt ctgtaaacat tgtgcatctg tccatgaaac attgatttct
     5461 tttgcagtat taatccgttt ttttcggcaa cacgtacaga ttgcgtattt tccggcaaaa
     5521 taagactaat tacagaaggt atttttaact tctcttttgc atactgcaga cagccttttg
     5581 cggcctcggt gccgtatcct tgaccccaaa aaagatcgtc gattcgatat ccgacttcga
     5641 tctcatccgt atcatcaata ttttgtttaa gcaatccgca aataccaaga aactttaagt
     5701 catcttttcg aattacagcc cagaatgaga tgccgtcgtt tttttgtctt tttctgatta
     5761 tctccagata gtccctggac tctttccggt ttaaggtttt aggaaaaaaa gtatgcactt
     5821 tctcgtttgc caacagattg gccaaatctt caaatctgtc ttccccgatc tcttcgagaa
     5881 acagccgtcc ggtttccagt attttcatat tttttttgtt gctcctgctg taaaataatt
     5941 aaaattaacc tgtaattgat aataaatcaa taacattctg attgtgatat atctatactg
     6001 tctgaattta ttaaaatgct gctgtatgca gactttgtga ttaaagctct tgcaatattg
     6061 gctatattaa tagttgctga atagtcattt cttaaagcag acatgtttat gcagtattaa
     6121 gaaataaatt taaatttcgc tatataaagg gtcaggtgtt tcaatgaaat taatgctaaa
     6181 cgatgagatc ttcactaaaa gatatcgcaa gtttctgttt cagttcccat tattgctgat
     6241 tattgtttct gttttgggag cttttattta tcatagtcag actatttttt ttgaaaaagg
     6301 agatatctcg tcaataataa atattgactt gcttaccagc tccttttttc acggttcgat
     6361 agtggctgtg gttatctatt ttattaagag ccgaaaactg gagaaaattt ttaaagaaaa
     6421 gccttcagat gttgctgaaa acaagataga tacgtttctg ccctgtctgc atggagtctg
     6481 gccttatcag tatcaggatg cttttttaac taccgaaggt acggctctaa gactttacgt
     6541 aaaaaaagtg ggcggatttg taatggtttg tgaatggaat aacctctcaa tgttaaaaat
     6601 cgaggctgta agggagccgc tgaatccttt tcttcctgtt ttatttaata cttcaaaaag
     6661 catcagaatc tctgatggaa agaaatcaaa aaagattctg tttgctgaac cggaacagac
     6721 cgctgaggag ttaagccggt tagtgaaaag catgtagttg taatctgtac ttctgacagg
     6781 ttcgatattt ctacataaaa gcggtagcag atgattttcg cgcagggact acatccgtgc
     6841 gtttctgaat tcatatttct gacaacaaga ttccgtcatc gaggggagaa aaacgttttt
     6901 ttccgggaaa gagatttata acaattatac tgagattgat ccatttatca ggcaatttac
     6961 gggatctcaa cgacagaatg cgtttaacgg gtataaatca tggccttgta taaaaacaaa
     7021 tatcgtgttg aatcggtacg tttgaaggga tgggattatt catcaccggg acattatttt
     7081 attaccatct gtacaaaaaa cagggaatgt ctgttcggaa atatcgtaca aaacaaaatc
     7141 gttttatcag atatgggaaa aattatggaa cgatattggc gcgaaatccc cgaccatttt
     7201 aatggtgtta aattggatga atacgtcata atgccaaatc atattcacgg aatcatatcc
     7261 atcatggtag agacgcccaa tttgggcgtc tctacgggaa ccgatcccga aacgggaacc
     7321 gatcccgaaa cgggaaccga tcccgaaacg ggaaccgatc ccgataaaaa aaataccccg
     7381 aatatcggaa tcattatcaa tcaatacaaa cgaatttgta caatacgtat tcgtgaatca
     7441 tatcccggaa taataatttg gcaaacgcgt tatcatgatc atatcattcg tgatgaaaat
     7501 gaactatacc ggatccgcca atatatcata aaaaatcctg caaattggga aaaagatgat
     7561 aaatataaaa aggagccttg ccggacatca cgttaatcat tatcaatcaa tacaaacgaa
     7621 tttatacaat acatattcgt gaatcaatcc ccggaatatt gatacaaaat ccgcattttc
     7681 acaacctgcc tttttttaat aaaagttgat tctccttttg tttgccctta ttattacaaa
     7741 gcgggtgact ttatcacctg gtccgtcctt gaaacgggtt tcactgctct ttgtacacca
     7801 tcagaactat ttatcaatca agtactccct gtttaaacat taaaccaaat ataatatggt
     7861 ctgtttgagt gtgcctaaga aaatctctaa acggagactg ttatgtttaa aaaaatgaag
     7921 cttggaacca aactgataac aatcactggt gcgataataa taacaacggc tctggttata
     7981 accgtggctg ctgttgtcca atcatctaaa gctctatcca acgaagcttt taacaaatt
//
LOCUS       DAOTGZ010000125         8009 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_3064
            NODE_10849_length_8009_cov_4.113402, whole genome shotgun sequence.
ACCESSION   DAOTGZ010000125 DAOTGZ010000000
VERSION     DAOTGZ010000125.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868088
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 8009)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8009)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:22:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,785
            CDSs (total)                      :: 2,751
            Genes (coding)                    :: 2,732
            CDSs (with protein)               :: 2,732
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 12 of 19
            Pseudo Genes (internal stop)      :: 5 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8009
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10849_length_8009_cov_4.113402"
                     /isolate="AMR_MDS_3064"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1616
                     /locus_tag="P5123_08840"
     CDS             <1..1616
                     /locus_tag="P5123_08840"
                     /inference="COORDINATES: protein motif:HMM:NF012869.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HRX16407.1"
                     /translation="IQKGIDYGIAPKDIDYLVKISIITAIVLFLQFFSFRYQGKLMMK
                     IGNRILYYIRRDLFNHIQGLSFRFFDKNKTGNIMTRLTVDVQVLEELLMTGLDTVLVD
                     VIMIVGIIGAMLLLDLKLSLVLLFILPLLVMIVFGLQSRIVRAARGIQGRLSSVNAFL
                     NESLSGVMVTRSFAREERNIKLFESYNGNYYKNTRKFYPLHAYFWQSVIMLNTASAGL
                     VIIGGGLLIKNGQVTIGVVAAFLTYVTQLFQPMQKISNMLNQLSRAYASCERIFAIMD
                     EKPDIVEKANSISDSKLTGDVTFKNVKFHYNPEEPILKGICFDAKAGETVAIVGHTGS
                     GKSTIVHLVSRFYDVADGAVLIDSIDIRDYSIAAYRRQTAVVMQEPQIFSGSVFENIS
                     FSVPDSTIDDVMAVCKQLGIHEMIIDFPDGYDTPMGERGANISIGQKQLLAFARAMLR
                     DPRILILDEASAYLDTRTETMVQKALASIMKGRTTFVIAHRLSTIRNANKIIVLDKGI
                     ITDIGSHEELLKKEGIYKNFLRQNNPIAG"
     gene            1736..2155
                     /locus_tag="P5123_08845"
     CDS             1736..2155
                     /locus_tag="P5123_08845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004434189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030551 - cyclic nucleotide binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic nucleotide-binding domain-containing
                     protein"
                     /protein_id="HRX16408.1"
                     /translation="MKVVSFKYGQTVIKQGDKENRMYVILEGEVEIRLRSGENEIVVT
                     HLRKNDFFGEMSLFRNRPRSADVIARTDVKLAYIESLQQLQKFLVNNPAFAFKMVLIL
                     AERLGRTDELLIGKVSEANRLKLTSFVDKDEDLFEDL"
     gene            complement(2152..2625)
                     /locus_tag="P5123_08850"
     CDS             complement(2152..2625)
                     /locus_tag="P5123_08850"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRX16409.1"
                     /translation="MRSVMLLGVTALILASCKQNGALSHEELFLNLRATYHKSDIQNF
                     KQLILTSDKKYISQKATRINKLSKAAKNELADNFKIDVNLINFKSIDDYLRFYFILSK
                     TSNRDILSTAINCNIARIEANERNAKYYMTNGINLYFTKEERYWKFCYEKSLNEN"
     gene            complement(2613..3482)
                     /gene="panC"
                     /locus_tag="P5123_08855"
     CDS             complement(2613..3482)
                     /gene="panC"
                     /locus_tag="P5123_08855"
                     /inference="COORDINATES: protein motif:HMM:TIGR00018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004592 - pantoate-beta-alanine ligase
                     activity [Evidence IEA];
                     GO_process: GO:0015940 - pantothenate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pantoate--beta-alanine ligase"
                     /protein_id="HRX16410.1"
                     /translation="MQITSSIKTLRQWLKQDCTQNGLTGFVPTMGALHQGHISLINRS
                     VDSCERTVVSIFVNRIQFNDKNDYNNYPNTLSEDLQKCSQAGVNYVFVPDESVMYNNH
                     KTYIDVADLTDNLCGKHRPGHFKGVFTVVSKLFNIVQPDMAFFGRKDLQQALAVRKMV
                     CDLNFPIKIIICPTIREKDGLALSSRNVHLDTKQRERALSIYKSLMLAKKMILDGTTD
                     LDLVCTRIHEFIVQNGFPESIDYITAVNIDSLEPVSVLDQESAIALAANFGSVRLIDN
                     MIIYTDGDTRCDL"
     gene            complement(3484..4338)
                     /locus_tag="P5123_08860"
     CDS             complement(3484..4338)
                     /locus_tag="P5123_08860"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024880.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HRX16411.1"
                     /translation="MENYSCICCGEPRINYFFSKPSRFGEIFHIHKCPNCGSESIQPL
                     PSDDMLKDYYEKAYFTTRTERGYDNYFSNRIKNEIERVFKMNLKDIGFFSDITLANKG
                     VHLDIGCAAGYFTAYMKSLGWNANGIDISHICTDFARNELKLNVICDDYLKHKYKHKF
                     NLITMWASIEHLSRPDNFLNKIKHDLTDNGKLYISTCRADSFFKNVARKRWRYYNVPE
                     HIHYFTISGIKSLLERNGFNTVKLFCYGSGFGKPGSFIRRSADFLAKKICAGDMMVIS
                     AQKARVEN"
     gene            4666..5733
                     /locus_tag="P5123_08865"
     CDS             4666..5733
                     /locus_tag="P5123_08865"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRX16412.1"
                     /translation="MSHYDYSRKALLFSSDRRVEMQLRKSLSFISIELEKNIPNLSSL
                     NTIKTVAKSLKGTGFMRAELFNFIQTTGFPYLVIMDFRLDLGLDTQNDPGNLKLLKTF
                     LISYIIFSMGRGFDKLHLNLLLIYNQDDTASAEELSEKPERLLSLLHTKNDEVNALIS
                     RMKNDNTLFKKIFSIRFVARQGIENSLDETIKTFNSSITARNNLTNERVKKSLGAVQK
                     GEKNAADVYYLCGQNCYQNGTVCSDVPTRYQIASPGSLYVEGYWISTTTREVADRIIK
                     SVATALKAKGIQKGEDLVIHLQEKCIIDGTTATALTGLIIQHLQKLYNGVSVKVSRFN
                     EPILKNSQGFSMIKEYLVYED"
     gene            complement(5787..6482)
                     /locus_tag="P5123_08870"
     CDS             complement(5787..6482)
                     /locus_tag="P5123_08870"
                     /inference="COORDINATES: protein motif:HMM:NF012905.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IA family hydrolase"
                     /protein_id="HRX16413.1"
                     /translation="MTNNKCTALLFDLFHTLSSIRHTHGPGDDTADILGIDKQKWREA
                     LFNRSEARLRGEITDPVKIIRDVSDKIDPNIPEQIIIKAAKSRYSRFEKSLVTPAQHV
                     VETLQSLRDRGYKTGLVSNADVMEVFAWDKSPLSKCFDFTVFSCHCGYVKPEPSIYNL
                     CIKGLGKQANDCVFIGDGGNNEHAGAKECGMRTVFTSEFVKDLWPEKIEMLKSKADYH
                     ITDIRELLDANFL"
     gene            6850..7983
                     /locus_tag="P5123_08875"
     CDS             6850..7983
                     /locus_tag="P5123_08875"
                     /inference="COORDINATES: protein motif:HMM:NF012692.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family transcriptional regulator"
                     /protein_id="HRX16414.1"
                     /translation="MIQIDGVYQKVVKQSNRRKIFNYIRKKRVVTKLDIARDLDLSIT
                     TVSSNVSWLTNKGFVTDNNHDSSTGGRKARLIEFNPSACFSIGVEFKETHARIVMIDL
                     DYKIINESILESLNLSTIPDQTMQMIDRFISGNNSDKRIAGIGISVPGIVDKEHRMLL
                     NAPNMEVQNIDFSSVVQKYNPLPVYIENEANCAAFAEFKDSDSKGTLCYVSITEGIGG
                     ALIINNRLITGTSNRAGEFGHTVIERGGRKCHCGNSGCWEQYASEQALRRMFAEKGID
                     LSIDMAFDKYGEDSKITEIIDNYAGQIAIGLYNLFMVFDPGVIIVGGSIARHESVLKP
                     LVTDWISRHTRLLTDREVPVIYSSFGGDAGVIGAGVFPFTEIL"
BASE COUNT         2415 a         1384 c         1588 g         2622 t
ORIGIN      
        1 ttatccaaaa gggtatcgac tatggaattg ctcctaaaga tatagactat cttgtaaaaa
       61 tatctattat tacagccatt gtactttttt tgcagttttt ctcttttcgt tatcagggga
      121 agctgatgat gaagattggc aatcgcatac tttactatat aaggcgtgac ctttttaacc
      181 atattcaggg gttatcattc aggtttttcg ataaaaacaa aaccggtaat ataatgacgc
      241 gcctcactgt agatgtgcag gttctagaag agcttttaat gacagggctt gatacagttt
      301 tggttgatgt cattatgatc gtaggaatta ttggggcaat gcttctgctt gatctaaaac
      361 tgagtctggt tcttctgttt atcttgcctc tgcttgtgat gattgttttc ggtcttcagt
      421 caaggatagt ccgggctgcc cgtggtattc agggaaggct ctcttcggtt aatgcttttc
      481 tgaacgaatc tctttccggt gttatggtta ctcgttcctt tgcgcgtgag gagcgtaaca
      541 taaaactttt tgagagctat aatggaaatt attacaagaa tacacgaaag ttttatcctc
      601 ttcacgccta tttttggcaa agtgtgatta tgctgaacac tgccagcgcc ggattagtga
      661 ttattggcgg tggtctattg attaaaaatg gtcaggttac tatcggagtt gttgctgcat
      721 ttttaaccta tgttacacaa ctttttcaac cgatgcagaa gattagcaat atgttaaatc
      781 agcttagccg agcatacgcc tcttgtgagc gtatttttgc aattatggac gaaaagcccg
      841 atatagttga aaaagcaaat tcaataagtg attctaaact gacaggtgat gttactttca
      901 aaaatgtaaa atttcactat aatcctgaag aaccaatatt aaaaggtatc tgttttgatg
      961 cgaaggcggg tgaaacagtt gctattgtcg gccataccgg atctgggaaa tcgacaatag
     1021 tacatcttgt cagtcgtttt tatgatgttg cagacggcgc agttttgatc gattcaatag
     1081 atatcaggga ctattccata gcggcatatc gacgacagac tgctgttgtt atgcaggaac
     1141 cacagatttt ctcgggttct gtctttgaaa atatctcttt ttcggtacct gattcaacca
     1201 ttgatgatgt gatggctgtt tgcaaacagc ttggaataca cgaaatgata attgactttc
     1261 cagatgggta cgacactccc atgggtgaga ggggcgcaaa tatatcgatt ggacaaaaac
     1321 agctcttggc ttttgctaga gccatgcttc gtgatccacg catactcata cttgatgagg
     1381 ccagtgctta tttggatacc cgtactgaaa caatggttca aaaggctctt gcgagtataa
     1441 tgaaaggccg taccactttt gtaattgctc acaggctctc tacaattcgc aatgccaata
     1501 aaataattgt gctcgataaa ggtataataa cagatatagg ctcacatgaa gagctgctta
     1561 aaaaagaggg catctataag aattttttaa gacaaaataa cccgattgcc ggttgatact
     1621 attatacgaa tctggatttt attccgttta aaattatcct tcactcttga ctttgctcgc
     1681 ttttgtcgat attagtaata gtgacgaaat tgccattaaa tacaatggag aatatatgaa
     1741 ggtagttagc tttaaatacg gacaaactgt tattaagcag ggtgataaag agaaccggat
     1801 gtatgttata cttgagggcg aggtcgaaat aaggcttaga agcggagaaa atgagattgt
     1861 ggtaacacat cttagaaaaa atgatttttt tggagagatg tctcttttta gaaatcgacc
     1921 aaggtcggcg gatgtgatag cacgtacaga tgttaaactt gcatatatcg agagtcttca
     1981 acagcttcag aagtttttag taaataatcc ggcttttgcg tttaaaatgg tgctgattct
     2041 tgctgaaaga ttagggcgta ctgatgaact tttaattggt aaagtatcag aggcaaatag
     2101 actcaaactg acttcatttg tagataagga cgaagatcta tttgaagatc tttagttttc
     2161 attcaaactt ttttcataac agaatttcca gtagcgctcc tctttcgtaa aatagagatt
     2221 gattccgttt gtcatataat attttgcgtt cctttcgtta gcctcaattc gtgcaatatt
     2281 acagtttatg gcggtgctta aaatatcgcg gtttgacgtt ttgcttaata tgaagtaaaa
     2341 acgaagatag tcatcaatac tcttaaaatt gataagattt acatctattt taaaattatc
     2401 cgctagttca tttttagctg cttttgaaag tttgttaatc ctggtggctt tttggctgat
     2461 atacttttta tcagaagtta atatcagctg tttaaaattt tgaatatcgc ttttatggta
     2521 agttgctcgc aaatttaaaa aaagttcttc atgtgaaaga gcaccatttt gtttacatga
     2581 ggctaaaata agagcagtaa cacctaatag catcacagat cgcatcttgt atctccatcg
     2641 gtataaataa tcatgttgtc aatcaaccgt acggaaccaa aattggcagc tagtgcaata
     2701 gcactttctt gatctaaaac cgatactggt tcaagtgagt cgatattaac tgctgtaata
     2761 taatcgatac tttcagggaa tccgttttga acgatgaatt catgtatacg tgtacaaaca
     2821 agatcaaggt ctgtagtccc atcaagtatc atctttttcg ctagcatgag ggatttataa
     2881 atagatagcg cgcgttcacg ctgttttgta tcaagatgaa cgtttcttga acttaaagct
     2941 aatccatctt tttctcttat cgtagggcag ataataatct ttatcgggaa gttaaggtca
     3001 cagaccattt tcctaacagc aagtgcctgt tgaaggtctt ttcttccgaa aaacgccatg
     3061 tctggttgta caatgttaaa gagtttgcta acaacagtaa aaactccttt aaaatggcca
     3121 ggcctgtgtt ttccgcaaag attatctgta aggtctgcta catcaatata ggttttgtga
     3181 ttattgtaca tcacggattc atccggtaca aacacatagt tgactccggc ttgggaacat
     3241 ttttgaagat cttcggaaag agtattcggg taattattat aatcgttctt atcattaaac
     3301 tgtatgcggt ttacaaatat gctaactaca gtacgttcgc agctgtcaac tgaccgattt
     3361 ataagagaaa tatggccttg atgcagagcc cccatggttg gtacaaatcc tgttaggccg
     3421 ttttgggtgc aatcctgttt tagccattgc cttaacgttt taatcgatga agtaatttgc
     3481 atctcagttc tccacgcgtg ctttctgcgc gctaatcacc atcatatctc cagcgcagat
     3541 ttttttggct aaaaaatcgg cacttcgtct aataaaactg cctggtttac caaatccact
     3601 tccgtaacag aacagtttta cggtattaaa accgtttctt tcaagtagcg acttaattcc
     3661 agatattgta aaatagtgaa tatgttcggg gacattgtaa tagcgccacc tttttcgggc
     3721 aacattttta aaaaaagagt ctgcgcggca tgtcgaaata tatagttttc cgttatcggt
     3781 aagatcatgt ttgattttat ttagaaaatt atccggacgg gataaatgtt ctatgctagc
     3841 ccacatggtt atcagattaa acttatgttt atacttatgt tttagataat catcacagat
     3901 gacattgagt ttcagttcgt ttcgagcaaa atcggtacaa atatgcgata tgtcaattcc
     3961 gttagcattc catcctaacg acttcatata ggcagtaaaa tatcctgccg cgcatcctat
     4021 atcaagatgt actcctttat tagccagagt aatgtcggaa aagaatccaa tatctttaag
     4081 attcatcttg aaaacccgtt caatctcgtt tttgatccgg ttagaaaaat agttatcgta
     4141 gccgcgttcg gttctggtgg taaagtaagc tttttcgtag taatctttaa gcatatcatc
     4201 agaaggaaga ggctgtatgg attcactgcc gcaattcggg catttatgta tgtgaaatat
     4261 ctctccaaat cgtgatggtt tcgaaaaaaa ataatttatt cgtggttcac cacagcaaat
     4321 gcatgaatag ttttccatta tttctcctga attgttaata atgctaagtt attatttttt
     4381 aaaaaaatgc tgcttttcta aattctcttt tgattatcca taaaacaatg ctatattata
     4441 taacgttata cttatacttt cgataattcc taatcagagc ttagtattga aactataact
     4501 taattacaag atttatattc gtttgtctaa gagagttttg tccatttaaa aaaagaataa
     4561 ttgacaccta tagggtattt tttttaaagt attaataaat gattaaaacc tgtgaaaatt
     4621 tatacgattt ataaagatcg ttgatatgta aatgccggta ccgcaatgtc acattatgat
     4681 tattccagaa aagcccttct attctcgtcg gacagaagag tagaaatgca gttaagaaag
     4741 agtctttcgt ttattagtat tgaacttgaa aaaaacatac caaatctttc ttcattaaat
     4801 accataaaaa ccgttgcaaa aagtttaaag ggtactgggt tcatgagagc cgagcttttt
     4861 aattttattc aaactaccgg ttttccgtat cttgtaataa tggatttcag gcttgatctt
     4921 ggacttgaca cacaaaatga tcctggtaat ttaaaactgc taaagacatt tcttatttct
     4981 tacattattt tcagtatggg gcgtggcttt gacaaactac atctgaatct tctattaatt
     5041 tataatcagg atgacactgc ctctgccgaa gagctttcgg aaaagccgga acgtctgctt
     5101 tctcttttgc acaccaaaaa tgatgaggtt aatgccctta tttcccgaat gaaaaacgat
     5161 aacactctgt ttaagaaaat tttttcgata aggtttgttg caaggcaggg tatcgaaaac
     5221 agtctggacg aaacaataaa aacttttaat agctcgataa cagcaagaaa taaccttaca
     5281 aatgaacgtg tcaaaaaaag tctgggtgct gtgcaaaagg gcgaaaaaaa cgcggcagat
     5341 gtctattatc tatgtggtca gaactgttac cagaatggaa ctgtttgctc ggatgttcct
     5401 actcgttatc agattgcttc ccccggtagc ctttatgtag agggttactg gatttctaca
     5461 acaacacggg aagttgccga tagaataata aagtcggtag cgaccgcctt aaaggcaaaa
     5521 ggcattcaaa agggagaaga tctggttatt catcttcagg aaaaatgtat aatagacggc
     5581 acaacagcta cagccttaac tggactaatt attcagcatt tgcaaaaatt atataatggt
     5641 gtgtcggtta aggtctcacg tttcaatgag cctattttga aaaattccca aggtttttca
     5701 atgattaaag agtatcttgt ttatgaagat tagaagattc tactttcaac actgagaaaa
     5761 tatcaaggta tagatttttg tctttgttac aggaaatttg catctaaaag ctctctaatg
     5821 tcggttatat gataatcggc ttttgatttt aacatttcta ttttttcagg ccagagatct
     5881 tttacaaatt cactggtaaa tacagtgcgc attccacact ctttagctcc tgcatgttcg
     5941 ttgttgcctc cgtcaccaat aaaaacgcaa tcattggctt gtttacctag cccttttatg
     6001 caaagattgt atattgatgg ttcaggtttt acatatccgc aatgacacga aaaaacggta
     6061 aagtcaaagc atttgctcag agggctttta tcccatgcaa aaacctccat cacatccgca
     6121 ttgctaacaa gccctgtttt atagcctctg tcacgcaaag attgtaatgt ttcgacaaca
     6181 tgttgagccg gggtgaccag tgatttttca aaacgtgagt agcgggattt cgcagcttta
     6241 ataatgattt gttctggtat atttggatca attttatcgc taacatcacg aattatttta
     6301 accggatcgg taatctctcc ccgcaatctt gcttctgatc tattaaagag tgcttctcgc
     6361 catttttgtt tatcaatacc aagtatgtca gctgtgtcat caccagggcc gtgagtatgt
     6421 cgtatcgatg agagggtatg aaaaagatca aataacagag ccgtgcattt gttgtttgtc
     6481 atgatgttcg ccttaactta atttcaatat tcatctgtat tgtcaatata ttgcgggtca
     6541 agcaaaagaa tgatttgcgt acatcattta gggcaattgt ttttgattat aattttactt
     6601 gcaacaaaca gggggtgatg tcaatttgat ctattactgc tcgattgtca tgatttactg
     6661 tagacgcagt attaagtaat atttgtctct tattttaaaa ctgtttatat gggtaatcac
     6721 taataaattt ttaattataa aagatagggt taaattaata ttgtgtctta tggaaaaaaa
     6781 gaggtgctgg gctgttcgca tttatggctt ggttgataaa tcaattttaa gaggttacca
     6841 ggggttatca tgattcagat agacggagta taccaaaaag ttgtaaagca gagtaatcgt
     6901 cgtaagatct tcaattatat tcgcaaaaaa cgagttgtta caaaacttga tattgctcgt
     6961 gacctggatc ttagcataac aactgtttcc agtaatgtaa gttggcttac aaataagggg
     7021 tttgtaacag ataataatca tgacagcagc actggagggc gtaaggctag gttaatagag
     7081 tttaatccat cagcatgttt ctctattgga gtggagttta aagagactca cgcgcgtatt
     7141 gtcatgattg atcttgatta caagataata aatgaatcta ttctcgagtc acttaattta
     7201 agtactattc ccgatcaaac aatgcagatg atcgacaggt ttatttcagg caataatagt
     7261 gataaaagaa tagccggcat tggaatttcg gttcccggta tcgttgataa agagcatcgt
     7321 atgctgctta atgctcctaa tatggaagtt cagaatatag atttttcctc tgttgtgcaa
     7381 aagtataacc ctttaccggt atatatcgaa aatgaagcaa actgcgcggc tttcgcggaa
     7441 tttaaagata gtgacagtaa ggggacttta tgttatgtct ccattaccga aggaatcgga
     7501 ggggctctga taattaataa tcgcttgatt accggaacct ctaaccgtgc cggagaattt
     7561 ggccatactg ttattgagcg tgggggaaga aagtgccatt gcggaaacag tggctgttgg
     7621 gaacagtatg cctctgaaca ggcgctccga cgtatgtttg ccgaaaaggg tattgacctt
     7681 tcaattgata tggcgtttga taaatacggc gaggattcaa aaatcactga aattattgac
     7741 aattacgccg gacaaatagc aataggcctt tataatctct ttatggtatt tgaccctgga
     7801 gtcataatcg taggtggttc aattgcacgg catgagtctg tcttgaaacc tcttgtgact
     7861 gactggatat ctcgacatac acgattgtta accgatcgcg aggttcctgt tatatattcc
     7921 tcttttggcg gtgatgctgg ggttataggc gcaggagtat ttccttttac cgaaattctg
     7981 taaacgataa gaacagttat taccaaaaa
//
LOCUS       DAOTGZ010000126         7995 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_3064
            NODE_10871_length_7995_cov_5.107053, whole genome shotgun sequence.
ACCESSION   DAOTGZ010000126 DAOTGZ010000000
VERSION     DAOTGZ010000126.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868088
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 7995)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7995)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:22:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,785
            CDSs (total)                      :: 2,751
            Genes (coding)                    :: 2,732
            CDSs (with protein)               :: 2,732
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 12 of 19
            Pseudo Genes (internal stop)      :: 5 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7995
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10871_length_7995_cov_5.107053"
                     /isolate="AMR_MDS_3064"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(173..1210)
                     /gene="dgcA"
                     /locus_tag="P5123_08880"
     CDS             complement(173..1210)
                     /gene="dgcA"
                     /locus_tag="P5123_08880"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014454926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase DgcA"
                     /protein_id="HRX16415.1"
                     /translation="MSELNHEQLEKYERQIFDLRQLIEISKGLNSTLEYNMLIESILL
                     TCMGQMQLIKAGIFLKKGIDEDAYILHRNYKGFDIDHDTDYEILGASPIIDLFENHDR
                     CWTLEEIKVEVDDKLSCLECLKKINPSLVVPLKSRGKMNGIIILGERINQLDFCEEEK
                     LYLLDIASLAGIAIENAYLYELATTDMMTKLKIHHFFQRLLIEEREKAKKRKKPLILM
                     MLDIDHFKDFNDKYGHLCGDLVLKNVANVIKNNCRQIDIAARYGGEEMAIILPKTDIE
                     AGIIAAERLRRGIEHAVVEHEGKRLSVKVSIGLTQLRNEDVTNDDLIGRADRALYMSK
                     ENGRNMVSYIL"
     gene            complement(1215..1844)
                     /gene="hisH"
                     /locus_tag="P5123_08885"
     CDS             complement(1215..1844)
                     /gene="hisH"
                     /locus_tag="P5123_08885"
                     /EC_number="4.3.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013038364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000107 - imidazoleglycerol-phosphate
                     synthase activity [Evidence IEA];
                     GO_function: GO:0016884 - carbon-nitrogen ligase activity,
                     with glutamine as amido-N-donor [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="imidazole glycerol phosphate synthase subunit
                     HisH"
                     /protein_id="HRX16416.1"
                     /translation="MISIIDYGMGNLRSVVKAFELFTPDVRIINDPRDIESSSAVVLP
                     GDGAFGMAMQNLQDGGWVDPVFSYINSGKIFVGICVGYQLLFESSEEFGYSKGLGLIP
                     GNVKKFDYHDLKVPHMGWNNVKFTKPNPFIEGIEDNSYFYFIHTYYPEIKDISWVCGE
                     TEYGALFSSIVCNDNVFGTQFHPEKSHKAGLKIIENIVNLTGCDTTKER"
     gene            complement(1898..2488)
                     /gene="hisB"
                     /locus_tag="P5123_08890"
     CDS             complement(1898..2488)
                     /gene="hisB"
                     /locus_tag="P5123_08890"
                     /EC_number="4.2.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002719406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004424 - imidazoleglycerol-phosphate
                     dehydratase activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="imidazoleglycerol-phosphate dehydratase HisB"
                     /protein_id="HRX16417.1"
                     /translation="MKRSAKASRKTAETDITVSVELLSTKPSVISSGVPFFDHMLQAL
                     ARHGRMHIDICCKGDTHIDDHHSVEDIGIVLGDALKKALGEKRGIRRFGFGSVPMDDS
                     LTQVSVDLSGRGYFHYNGETLNGKIKNYDEELTHEFFYALAHHAGINLHLNLLYGRNR
                     HHIHESFFKSFATAFYKAVSFDKISIDDIPSTKGMI"
     gene            complement(2488..5223)
                     /locus_tag="P5123_08895"
     CDS             complement(2488..5223)
                     /locus_tag="P5123_08895"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRX16418.1"
                     /translation="MFFLVNSCKYDHDFYYNKIEHYKLTCILDSLGTCFFMLRKICFG
                     IIVSSLFIGISLSSAYAKNIPELNHLKTKQLDIIFAEGMEDAAHNTARIAMDELLFVS
                     DITGSRLGAPVTICLEDPLLPLLSRGLSQNYLVSPLRTYNKSRYILISSYPDYRSMRR
                     KIKSDLTDILLDDLLSSVRYRKNGIRVLWSDLSHEYRLALKELQFAFSDPDLPDPSLL
                     YNINSVGEISSGNNCKDKLLLALDYIVKNYGAETVGRITLRIINNISFAEALKAELSV
                     TSKQFLHELKSYYEIDIQKVLPSNGYHKKFILSANEKYAAVLDNDSFTIYTAKNGLPD
                     RSIVSYKYDKLKNEVPICFTSDSSFFAAAFHDGAKRGVVVYSLDAKKEIYRFMTGYRR
                     LYSASVDSELKTIVLNVSTGRSNEIHYLEIKDKKIKKIASGSFALRSPRITSDGKTVY
                     YISNQDPLGSYDRFSDSLFSYSMETGDGGVLLRGNYGLDSLLVNRNGSVFMLQKRKGR
                     RVLLVADEDGVRESESLSVGTDSLVNTSDRGVKVLINQNGNFITEYAGTMDISSFNSD
                     YNPEADLRYRSDEDLDKRQLTPYSVKTHLKEVETSIFSRGKDLAGAYTNVVIENSLSR
                     YAIYGAATYARGDKEDAPGAIMGAVYRGFPLDFSLEAFKITGLSPLRQSPAIIGFDSN
                     LYTLENTGGRVSISLITAKSFGFSGSIYGENQAVKTGSLEMTNKFYGSTITLFYDREE
                     GSAAFITDDAYITADYRYSYGEDNSGSLLLYGIGSKGIPVSTSFYAEAAMSGCYINSS
                     SDSGIGIDFFNFSDYLYNPVTVEYAFSSRAAIYFVPFRRYHSNFIRGSAGIGPFVSFM
                     LFKNNEDSTIREIFTGLSLRAGSNDRLFLKLDAFYTADYEDGEEFRLIITSGVRF"
     gene            5400..7268
                     /locus_tag="P5123_08900"
     CDS             5400..7268
                     /locus_tag="P5123_08900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015904169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HRX16419.1"
                     /translation="MLIVKNLSKSYGSKPLFLDASFTLTRGEKAGLVGRNGHGKTTLF
                     RIITGKEEADSGTIETPSGYRIGTVEQHLNFSHDTAVNEAASGLSDPDEKWKAEKILS
                     GLGFSDQDMQKNPSTFSGGFQVRINLAKALLSEPDLMLLDEPTNYLDITSIRWLIQFL
                     KTWQNELILITHDRSFMDSVITHTISIHRAAVKKIVGNTEKMYQQIIKEEEITEKTRV
                     NDEKRRKEVQKFIDQYRSKARIASRVQSRIKMLDKMSSSERLAAIEELDFSFNEAPFS
                     AKTLLTVENISFGYDKPLFSNLGFSVQKGDRICIIGKNGKGKTTLLKVLAGVLKPSTG
                     EIHTHQQTKSGYFEQTNISSLDPNRTVEQEIAASGTGEDAQLARNIAGSMMFKGDDAL
                     KKISVLSGGEKARVMLGKIVIQPANLLLLDEPTNHFDIQSCDSLIEALDSYEGAVIMV
                     THNEMFLRAIAQKLIIFNRGSVQFFNGTYDDFLQKYGWEEEDTNKFSGNITTDSAELS
                     ESQKLNNQINPKEARKIRSEIIRERSEILKPLEKEIDNLENIITKTEEKLEEIQQTLI
                     KASQSDDGSAIQKLSIESHNLNELLEKSFDSLEKTTLEYEEKKSWFEEKLTSIGQQ"
     gene            complement(7313..7639)
                     /locus_tag="P5123_08905"
     CDS             complement(7313..7639)
                     /locus_tag="P5123_08905"
                     /inference="COORDINATES: protein motif:HMM:NF013786.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0008897 - holo-[acyl-carrier-protein]
                     synthase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4'-phosphopantetheinyl transferase superfamily
                     protein"
                     /protein_id="HRX16420.1"
                     /translation="MVCVVFYDKPVGIDIEYIRERVMLNQLASRFFHKDELRKFKSLD
                     KDYSIEYFYRIWTRKESYLKFFGIGVGENLKEFSTEGKLKNDGKDVTIVSYIKGNYYI
                     SYAYQD"
     gene            complement(7851..7985)
                     /locus_tag="P5123_08910"
     CDS             complement(7851..7985)
                     /locus_tag="P5123_08910"
                     /inference="COORDINATES: protein motif:HMM:NF014517.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRX16421.1"
                     /translation="MYTGLYINGQVSVRRLNMFGDRQRIRLRTVNSVLFNLVKELEKL
                     "
BASE COUNT         2661 a         1622 c         1296 g         2416 t
ORIGIN      
        1 acaatttctt tgggtttccc tattttatta acagctgggg tatgaacttt tatgaaggcc
       61 aagaccaccc ttctggacaa caatccccaa aaatattgca tttgttaaat tgaaaggtgt
      121 tttgtcagga aaacaacttt taaaataaaa aacaaaagca ggaactgctt agctaaagaa
      181 tatacgaaac catgtttcga ccgttctctt tggacatgta gagagcgcgg tcagcacgac
      241 caatcaagtc atcatttgta acatcctcat tccttaattg tgtaagaccg atagaaactt
      301 ttacagataa tcttttcccc tcgtgctcca caacggcatg ctcaatacct cgtcttagtc
      361 gttcggcagc aataatacca gcttcaatat ctgttttcgg aagaataatc gccatctctt
      421 caccaccgta acgtgctgca atatcaatct gacggcagtt attttttata acattagcga
      481 catttttcaa cactaaatct ccgcataaat gaccatattt gtcgttaaag tctttaaaat
      541 gatcaatatc gagcatcatt aatataagag gttttttccg tttctttgcc ttctcacgct
      601 cctcaatcaa aagacgttga aaaaaatgat ggatcttgag tttggtcatc atgtcggttg
      661 tagcaagctc atagagatac gcgttttcaa ttgcaatacc tgcaagagag gcaatatcaa
      721 gcaaataaag tttttcttct tcacaaaaat ccagctgatt aatgcgctcg ccaagaataa
      781 taataccatt cattttgcca cggcttttca aagggacaac aaggctggga tttatttttt
      841 ttaggcattc aaggcatgat aatttgtcat ctacttcaac ttttatctct tccagtgtcc
      901 aacaacggtc atgattttca aaaagatcaa tgatgggcga agcaccgaga atttcataat
      961 cggtatcatg atcaatatca aatcccttgt aattacggtg aagaatatag gcatcttcat
     1021 ctataccttt tttcaagaat attcccgctt taataagctg catctgcccc atacatgtaa
     1081 gaagtattga ctcaataagc atattgtatt caagcgtcga atttagtccc ttgctgattt
     1141 caataagctg tcgcaaatca aatatttgcc gctcgtactt ttctaattgt tcatgattta
     1201 gttcagacat ttttttatct ttcttttgtt gtatcgcatc cggttagatt tacaatattt
     1261 tcaattattt taagtcctgc cttatggctt ttttctggat ggaactgtgt gccaaagaca
     1321 ttgtcgttgc acacaatact gctaaataac gccccatatt ctgtttcacc gcaaacccaa
     1381 cttatatctt ttatttcggg ataatatgta tgtataaaat aaaagtaact attatcttct
     1441 attccttcaa taaacggatt cggtttagta aattttacat tattccatcc catatgtggc
     1501 accttaagat catgataatc aaactttttt acattacccg gaataagccc tagcccttta
     1561 ctataaccga actcttcgct tgattcgaaa agtagttgat aaccgacaca tataccaaca
     1621 aatatcttac cagaattaat ataagaaaaa acagggtcta cccatccacc atcttgcagg
     1681 ttttgcattg ccatcccaaa agcaccatca cctggtaata caacagcact gcttgattcg
     1741 atatctcttg gatcatttat tattctaaca tccggagtaa aaagttcaaa agctttaaca
     1801 accgaacgaa gattccccat tccgtaatct ataatcgaaa tcacctttgt ttttctccta
     1861 aaataacaat ctgcaaaata acagaaacat tattgaatta aatcattcct tttgtagaag
     1921 gaatatcatc tatactaatc ttgtcaaaac taactgcctt ataaaacgca gtagcaaatg
     1981 atttaaaaaa tgattcatgt atatgatgtc gattccggcc atagagaagg ttcaagtgca
     2041 gatttatgcc tgcgtgatgt gcaagagcat agaaaaattc atgagttaat tcttcatcat
     2101 aatttttgat cttaccgtta agggtctcac cattgtagtg gaagtaaccc cgtcctgata
     2161 gatcaaccga tacctgagtc aaagagtcat ccattggtac cgatccgaag ccaaaacggc
     2221 gtattcccct tttttctcca agcgcctttt ttaatgcatc gccaagaaca atacctatat
     2281 cttcaacaga atgatgatca tctatatgtg tatcaccttt gcaacaaata tcaatatgca
     2341 tgcgaccatg gcgagcaagc gcctgaagca tatgatcaaa aaatggtaca ccgctagata
     2401 ttaccgaagg ttttgtagaa agtagctcta cagaaacggt tatgtctgtt tcggctgttt
     2461 ttctggaagc tttcgcggat cttttcatta aaatcggact cctgatgtaa ttattagcct
     2521 gaactcttca ccatcttcat aatcagccgt ataaaaagcg tcaagcttta aaaaaagacg
     2581 atcattagaa ccggctctta acgaaaggcc tgtaaatatt tccctgattg tactatcctc
     2641 attattttta aacagcataa aagagacaaa tgggccaata cctgcagatc cacggataaa
     2701 attagaatga taacggcgaa aaggaacgaa atatatagca gctcggctag aaaaagcata
     2761 ttcaaccgta accggattat aaagataatc agaaaaattg aaaaaatcaa taccaattcc
     2821 tgaatcagat gaactgttta tataacaccc cgacattgca gcttcagcat aaaaagatgt
     2881 agaaacggga atgcccttcg agccaatacc atagagaagt agagagccgc tattatcttc
     2941 tccgtaacta tagcgataat ctgcagtaat atatgcatca tctgtaataa aagctgcaga
     3001 accttcttct ctatcataga ataaggttat tgtcgaaccg taaaatttat tcgtcatttc
     3061 aagactacca gtttttacag cttgattttc tccataaatt gaaccactaa aaccaaaaga
     3121 ttttgctgta atcaaagaga ttgaaacgcg tcccccagtg ttttctaatg tataaagatt
     3181 tgagtcaaaa ccaataattg ctggcgattg tcttaaggga gataatccag taattttaaa
     3241 ggcttccagt gaaaaatcca aaggaaatcc tctataaact gctcccataa ttgcccctgg
     3301 agcatcttct ttatctcccc tagcataagt tgcagcgcca tagatagcat atctggataa
     3361 actattctcg ataacaacgt ttgtatatgc cccagccaaa tctttgcctc ttgaaaatat
     3421 agaggtttcg acttctttta aatgcgtttt taccgaataa ggagtcaact gacgcttatc
     3481 aaggtcttca tcagagcgat atcgcaaatc ggcttctgga ttatagtcgc tattgaaact
     3541 ggatatatcc atagttccag catattccgt tataaaatta ccgttttggt taatcaacac
     3601 ttttacacca cgatcactgg tatttactaa tgaatctgtt cccaccgaaa gagattcaga
     3661 ttcgcgtact ccatcctcat cagcaacgag aagaactcgc cgtccttttc tcttttgaag
     3721 cataaaaact gacccattcc tgttcacaag aagcgaatca agcccgtagt ttccacgcaa
     3781 taaaactccc ccatctccag tttccattga atagctgaac agactatcag agaaacgatc
     3841 gtagctaccc aagggatctt gattacttat atagtacact gttttgccgt cagaagtgat
     3901 gcgtggtgaa cgcagtgcaa atgaaccgga tgcaattttt tttatttttt tatctttaat
     3961 ttcaagataa tgaatctcat ttgatctgcc ggttgacaca ttaagcacaa ttgtcttaag
     4021 ttcagaatca accgatgctg aatagagtcg tctataaccc gtcataaaac gataaatctc
     4081 ttttttcgca tctaaagaat aaacgacaac ccctctcttt gcaccgtcat gaaatgccgc
     4141 tgcaaaaaat gatgaatcgg aggtaaaaca tattggaact tcgtttttta acttatcata
     4201 tttatatgag acaatagatc tgtcaggtaa gccatttttt gcagtataaa tagtaaaact
     4261 atcattgtcc aaaacagcgg cgtatttttc attagctgac aaaataaatt ttttatgata
     4321 tccgttacta ggcaagactt tttgtatgtc aatttcgtaa taacttttta gctcatgtaa
     4381 aaactgcttt gatgttacag aaagttcagc tttcaatgct tcagcaaatg aaatgttatt
     4441 tataattcgc aaagtaattc ttcctactgt ttcggcacca tagtttttta caatatagtc
     4501 aagtgctagt aatagtttgt ctttacaatt attaccgcta ctaatctcac ccacagaatt
     4561 aatattataa agaagactag gatcaggaag atcaggatcg ctaaatgcga actgcaattc
     4621 tttcaatgct aatctgtatt catgggaaag atcactccat agcactctaa ttccattttt
     4681 ccgatagcgg acagatgata aaagatcatc taacaaaata tcggttaaat cagatttaat
     4741 tttacgccgc attgatctat aatcaggata agaactgatt aaaatataac gagatttatt
     4801 atatgttctt aatggtgaaa caagataatt ctgagacaac cctcttgata acagaggcaa
     4861 aagggggtct tcaagacata ttgtaacagg tgcccccaac ctgctaccag taatatccga
     4921 aacaaaaaga agctcatcca tagcgatacg agcagtattg tgagccgcgt cctccattcc
     4981 ttctgcaaaa attatatcta gctgctttgt tttaagatga ttaagctcgg gtatgttttt
     5041 cgcgtatgca gaagacaaag agatacctat aaaaagtgaa gatacaatta tcccaaaaca
     5101 tatttttcgc aacataaaaa agcaggttcc aagactatca agaatacaag ttaatttata
     5161 atgttctatt ttgttataat aaaaatcatg atcgtatttg caagaattta caagaaaaaa
     5221 caatgttata tctccaacgc aggcagtaac tttctttcgt ctgccttttt ttctttactt
     5281 attaaacgat tctgtttatt tgacaaaata tttatggaac aatctatatc aaatgctcta
     5341 gatagatcaa aatattaaac aaatagtagc gattctgaac tattatgtga cggataagca
     5401 tgttaattgt caagaaccta tcaaaatctt acggatccaa acctcttttt ctggatgctt
     5461 ccttcacttt gactcgtggt gaaaaagcag gtcttgtcgg aagaaacggg cacggcaaga
     5521 ctactctttt cagaataata accggcaaag aagaggccga cagcggcacc attgagaccc
     5581 catccggcta ccgtattggt acggttgagc agcacctgaa ctttagccac gatacagctg
     5641 tcaatgaagc tgcaagtggt cttagtgacc ctgacgaaaa atggaaagca gaaaagattc
     5701 tttccgggct tggcttttca gatcaagata tgcaaaaaaa tccctccacc ttctccggag
     5761 ggtttcaagt ccgcattaat cttgcaaaag ctctcttaag cgagcccgat cttatgcttc
     5821 ttgacgaacc aaccaactat cttgatatta catcaatacg ctggctaatt cagtttttaa
     5881 aaacttggca aaacgaattg atactaataa cacacgaccg ttcatttatg gatagtgtta
     5941 taacacatac catatccatc catcgcgcag ctgttaaaaa aatagttggt aacaccgaaa
     6001 aaatgtatca gcaaataata aaggaagaag agatcaccga aaaaacccgt gtaaatgatg
     6061 aaaaacgccg caaagaagta caaaaattta ttgatcagta tcgctcaaaa gcccgtatcg
     6121 caagccgtgt acaatcacgt atcaagatgt tggacaaaat gagctctagc gaaagacttg
     6181 ccgcaatcga agagcttgat tttagtttta acgaagctcc cttttctgca aaaacccttt
     6241 tgacagtaga aaacattagc ttcggttacg ataaaccatt gtttagtaat ctaggatttt
     6301 cagtacaaaa gggagaccgt atctgtatta ttggaaaaaa tggaaaaggc aaaacaactc
     6361 tgctaaaagt tctcgccgga gttcttaaac caagcactgg agagatacat acacaccagc
     6421 aaacaaaatc cggctatttc gaacagacaa atatatcatc attggatcca aaccgtactg
     6481 ttgagcaaga aatcgccgca tccggtactg gtgaagacgc gcaacttgct cgcaatatag
     6541 ctggttcaat gatgttcaag ggtgacgatg ctctaaaaaa gatatctgta ctatcgggtg
     6601 gagaaaaggc gcgtgttatg ctaggtaaaa ttgttataca acctgccaat cttctcttac
     6661 ttgatgagcc tactaatcat ttcgatattc aaagctgtga ttcacttata gaagctctgg
     6721 acagttatga aggggcggta atcatggtta cacacaacga aatgttttta cgggcaattg
     6781 cccaaaaatt gattattttt aatcgtggca gcgtacagtt ctttaacggt acctatgatg
     6841 attttttaca aaaatatggc tgggaagaag aggacaccaa taaattttca ggtaacatca
     6901 cgacagacag tgcagaactt tcagagtcac aaaagcttaa caaccagata aatccaaaag
     6961 aggctcgaaa aatacgtagc gaaattatcc gggaacgatc ggaaattctt aaacctttgg
     7021 aaaaagaaat agataacctt gaaaatataa ttacaaaaac agaagagaag cttgaagaga
     7081 tacagcagac gcttatcaaa gcatcacaat ctgatgatgg atcggctata caaaaacttt
     7141 cgatagaatc gcacaacctg aatgaacttc ttgaaaaaag ttttgattct cttgaaaaaa
     7201 ctacacttga gtacgaggaa aagaaatcct ggtttgagga aaaacttacc agcataggtc
     7261 aacagtaact ctacttttat tcttaaaatt actaacttta tgcttctttt agttaatcct
     7321 gatatgcata agaaatataa taattaccct tgatgtaact aacaatagtt acatctttac
     7381 catcgttttt tagttttcct tccgtcgaaa actcctttaa attttctccc acgcctattc
     7441 caaaaaattt cagataactc tctttccgcg tccatattct gtaaaaatat tctatgctat
     7501 aatctttatc caaagattta aatttacgta attcatcttt atgaaaaaac cgtgacgcaa
     7561 gttggttcaa catgaccctc tccctaatat attcaatatc aattcccaca ggcttatcat
     7621 aaaataccac acagaccatt ctgcacgaat gtgatacatt aaaacaaaac ttgctattta
     7681 taaaaaacgg ctttccttga ttcccatatt gtatttgtaa atcattaata ccatataatt
     7741 tctgggcaat tttatctcta aattttgcgg catatcgact ctgtatttta gaagatggat
     7801 tacttccata ccatattgga atttttttta tataaatgtc aggcttcatc ttacaacttt
     7861 tcaagctctt taacaagatt aaaaagcaca ctgttcacag ttctcagtct aattctctga
     7921 cgatctccaa acatgttaag cctgcgaacc gaaacctgac catttatata taatcctgta
     7981 tacaccgttc caacc
//
LOCUS       DAOTGZ010000127         7984 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_3064
            NODE_10894_length_7984_cov_3.662000, whole genome shotgun sequence.
ACCESSION   DAOTGZ010000127 DAOTGZ010000000
VERSION     DAOTGZ010000127.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868088
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 7984)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7984)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:22:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,785
            CDSs (total)                      :: 2,751
            Genes (coding)                    :: 2,732
            CDSs (with protein)               :: 2,732
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 12 of 19
            Pseudo Genes (internal stop)      :: 5 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7984
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10894_length_7984_cov_3.662000"
                     /isolate="AMR_MDS_3064"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..711)
                     /locus_tag="P5123_08915"
     CDS             complement(<1..711)
                     /locus_tag="P5123_08915"
                     /inference="COORDINATES: protein motif:HMM:NF012382.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase
                     family protein"
                     /protein_id="HRX16422.1"
                     /translation="MALDFLKSIYEKKIALSAEISFNPYYAEISSALSQEIIINSKEF
                     IDLGSNNYLGLADNDYVKQEVKKAVDAYGISMCGTPIATGSHILYNKAVDALNSYTGC
                     EDGLIFPSCYQANAGLFQSLITPRDCVIFDQYVHSSLLEGIKATGARYFQYTHNDCEN
                     LRKRVQMLQKFENIFVVTESVFSTEGAVAPVKQIYSICNEYNATMVLDDSHGIGVLGN
                     NGKGILDHLFNLALLASQG"
     gene            complement(701..1906)
                     /locus_tag="P5123_08920"
     CDS             complement(701..1906)
                     /locus_tag="P5123_08920"
                     /inference="COORDINATES: protein motif:HMM:NF012927.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH:flavin oxidoreductase"
                     /protein_id="HRX16423.1"
                     /translation="MLFSPITIGTLKLKNRIVRSATFEGMADKDGNPTEEYIKYYQNL
                     LNQNIGLVITGFMYVNIEGKAIQPGQASLCSESVIPLYKKITKQKGDTKIFAQISHCG
                     RQTNRKVTGGHLWAPSKKRSLYFGEVPDMLSEEKINSIIRDYSESAYFAKLAGFDGIQ
                     LHAAHGYLIHQFILSSVNKRSDIYGIDSKTGIGTLFFEKVIEGVRNKCGEEFLISVKI
                     SGADDYGNTFDQPKLRELVRFLDTKNICLIEISYGTMDYPLNIIRGSDIPIDSILKYN
                     IKYKTDSIIKKFFFKKCLLPILKIKIKKNYKAYNEQYAQIAKDETSVPIILVGGLRSR
                     EEMEKQLRNKNCDMISMCRTFICENDLAVKLLNEKNYSSCCISCNLCLIMCDTDKPTK
                     CYQKVCNGS"
     gene            complement(1908..2918)
                     /locus_tag="P5123_08925"
     CDS             complement(1908..2918)
                     /locus_tag="P5123_08925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014902961.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016765 - transferase activity,
                     transferring alkyl or aryl (other than methyl) groups
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UbiA family prenyltransferase"
                     /protein_id="HRX16424.1"
                     /translation="MMAHILSGKLRKIIDFGYYYIKSMRLYYSFVTGISGWIGLEFYQ
                     HIASDYATVEVLTPTIKKIVIVIMLFGAWGINQIVNDFLGMKEDRINAPNRPMISGKL
                     NPFFAVLVSAIIMTAMGVVTLLYLEPVALIPLFTGCILNILYEYMKAYGIWGNIVFGL
                     MITQCTIFGFFASGPVGVVATKSRVSILLLLWIMNGIMTFYTYFKDYEGDKKNKIETL
                     VVKFDIKGARIIAFILSFVPILVFLFIYINDFVVARVNNVFIFLALVTLMLQVHNGYL
                     FYKYPKGDKTYYSLSNNFRAYVCAQAALISLFNKEMSLMLYIISYILIGFFFDLYKDT
                     LC"
     gene            complement(3483..3617)
                     /locus_tag="P5123_08930"
     CDS             complement(3483..3617)
                     /locus_tag="P5123_08930"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRX16425.1"
                     /translation="MRLLRKESLGLRHIAAIRPPQRLQLSYVENFKETIDHAYENEKN
                     "
     gene            complement(3652..3912)
                     /locus_tag="P5123_08935"
     CDS             complement(3652..3912)
                     /locus_tag="P5123_08935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010578294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toxin-antitoxin system HicB family antitoxin"
                     /protein_id="HRX16426.1"
                     /translation="MKTKASVLTLRIPIELKHKIEKVADQQGLSINQLALYAFTKEIQ
                     EMETYNSFSKHWKKKTKSELFEDFDSVICKIKEGTVPEWDKL"
     gene            complement(3902..4342)
                     /locus_tag="P5123_08940"
     CDS             complement(3902..4342)
                     /locus_tag="P5123_08940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010578293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004521 - endoribonuclease activity
                     [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putative toxin-antitoxin system toxin component,
                     PIN family"
                     /protein_id="HRX16427.1"
                     /translation="MIVVIDTNVLYQALRSSSGASNFILQLIRNHKITLALSLPVFSE
                     YEDVLKRPDKIKDLGLSTIDIDKILRFLAYISKPITIYYSFRPNLKDEEDNIFIELSL
                     ASNSDFIITSNTKDFIKSNELKFDDISIVTPAEFVMIWRKNYED"
     gene            4922..5593
                     /locus_tag="P5123_08945"
     CDS             4922..5593
                     /locus_tag="P5123_08945"
                     /inference="COORDINATES: protein motif:HMM:NF014724.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ElyC/SanA/YdcF family protein"
                     /protein_id="HRX16428.1"
                     /translation="MIRNNIKKIVLLFIIFPLIIFIFTIFTNLRILKYGSPVYTNIDT
                     CPQKYCAIVLGAGVVQNMYLSPVLEDRVKTAVALYKAGKVKKLLMSGANHTKNYNEVK
                     AMKNHAVKWGVNPRDIFLDYAGFSTYESMYRAQYIFGIKEAVIITQGFHVNRSVYLAK
                     KFDINAVGLVADRRRYIYIKQYRLREKLARVKAWIESVILRPLPSYLGDPIPISGDGR
                     VTHDL"
     gene            complement(5598..6593)
                     /locus_tag="P5123_08950"
     CDS             complement(5598..6593)
                     /locus_tag="P5123_08950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014164679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endo alpha-1,4 polygalactosaminidase"
                     /protein_id="HRX16429.1"
                     /translation="MKKFMQMLILVLIFLVSACSDDSSKDEENEDEDVDYREEMRDFV
                     ISISQYSKKLSPGFVIIPQNGIELVTLDGETDGPLSTDYLDAIDAHGQEDLFYGYDED
                     DAKSPDEDIQFISAFLDRSKDAGNTILVTDYCSTQSKVDDSYDLNSSAGYISFAAHER
                     DLNAIPTYPDPINNENSNNILSMSDVQNFLYIINGEEYSSKEDFINDVSNTNYDLVIM
                     DLFDNNQEPYSSSQIDELKTKANGSTRMVVCYMSIGEAEDYRYYWKDEWSNNSPDWMD
                     DENPDWEGNYKVKYWNEEWQKIIYGNDDSYLKMIIDAGFDGVYLDIIDAFEFFEN"
     gene            6734..>7984
                     /locus_tag="P5123_08955"
     CDS             6734..>7984
                     /locus_tag="P5123_08955"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA pseudouridine synthase"
                     /protein_id="HRX16430.1"
                     /translation="MLINSNYKCFRKLPESFNIGDLPESFCYPHQYMPHPVAIAAAKD
                     LINFLQSYELFEHDFGINENQTISEKNGLGKMFGVLIVKTSVGKLGYLAGYSGKIGER
                     NDHPGFVPPIADLISPGSFYRIGEEKLNRINEKIDKLQNSSTLCSLKKKLATLKTESE
                     EHLGNQRKILKTKKKEREIERNINKPKLSDDDYIKFCEELKSQSMSQQLRYKADRIDY
                     LEQIQNLEKQIAPLEEEIQSLKTKRKTFSAQLQQQIFDCFNFLNIKGEKMNIREIFAK
                     TSQKIAPSGAGECAAPKLLQYAFENNLTPITMAEFWWGRSPQSEIRKHGHYYPACRGK
                     CGPILKHMLKGMKVDDNPVVPLSVSNCDIEIIYEDDLIAVVNKPPGLLTVPGKDNHDS
                     LYSIMKDRYPGATGPLTVHRLDMST"
BASE COUNT         2663 a         1474 c         1319 g         2528 t
ORIGIN      
        1 gccctggctg gcaaggaggg ccaagttgaa tagatgatca agaatacctt taccgttatt
       61 tccaagaact cctattccgt gagagtcatc cagtaccatt gtcgcattat actcattaca
      121 tatactgtat atctgtttaa ccggagcaac cgctccttct gttgaaaata cagactcggt
      181 aactacaaat atattttcaa atttttgcag catctgtact ctttttcgaa gattttcaca
      241 gtcgttatgt gtatattgaa aatatcttgc gccagttgcc tttatgcctt caagtaaaga
      301 agagtgtaca tattgatcaa agattacaca atcacgggga gtaatcagcg actggaaaag
      361 tcctgcgttt gcttgatagc acgaagggaa aattagaccg tcttcacatc ctgtatatga
      421 attaagagca tcaacagctt tattataaag gatatgagag ccggttgcta tcggtgtacc
      481 acacatggat attccatatg catcaacagc tttttttact tcttgtttta cataatcgtt
      541 atctgcaaga cccagataat tatttgaccc aaggtcaata aactctttac tattgattat
      601 tatttcttgt gagagagcag aagaaatttc agcataatat ggattaaatg atatttctgc
      661 agacaaagca attttctttt cgtaaattga ttttaaaaaa tcaagagcca ttgcatacct
      721 tctggtaaca tttagtaggt ttatctgtat cacacataat caggcacaga ttgcagctaa
      781 tacaacatga agaataattt ttttcattta gcagcttaac agcaagatcg ttttcacata
      841 taaacgtacg acacatgctt atcatatcac agtttttatt acgcagctgc ttttccatct
      901 cctctctact tcgtaatccg cctacaagta taataggtac actggtttca tcttttgcaa
      961 tttgagcata ttgctcattg tacgctttat aattcttttt aattttgatt ttaagaattg
     1021 gaagaaggca ttttttaaag aaaaactttt taataatgct atctgttttg tatttaatat
     1081 tatattttaa aatcgaatct attggaatat cgcttcctct gataatgtta agaggatagt
     1141 ccattgttcc ataacttatt tctatcaaac agatattttt agtgtctaag aacctgacaa
     1201 gttcacgcag cttcggttga tcaaatgtat ttccataatc gtcagcaccg ctgatcttaa
     1261 ctgaaattaa aaattcttca ccgcatttat ttctaacgcc ttcaatcact ttttcaaaaa
     1321 acagggttcc gatacctgtc ttgctatcaa taccataaat atcactacgc ttatttactg
     1381 acgatagaat aaactgatgt atcagataac catgggctgc atgtagctga atcccatcaa
     1441 agccggccag tttcgcaaaa tatgctgact cagaataatc ccttataatc gaattgattt
     1501 tttcttctga aagcatatcc ggcacttcgc caaaatacaa tgatcgtttt ttagatggtg
     1561 cccataagtg acccccggta acttttctgt ttgtctgcct accacagtgg cttatctgtg
     1621 caaatatctt agtatcaccc ttttgctttg ttatcttttt atacaatgga attactgatt
     1681 cagaacatag tgatgcctgt ccaggctgaa ttgcttttcc ttcgatatta acatacataa
     1741 atccagttat tacaagaccg atgttctgat ttaaaaggtt ctgataatac ttaatatatt
     1801 cttctgtagg attaccgtct ttatcagcca taccttcaaa tgttgctgag cgcacaatac
     1861 gatttttcag ctttaaggtg ccgattgtaa ttggagaaaa aagcatatta acacagtgtg
     1921 tccttatata gatcgaagaa aaaaccgatg agaatatatg atattatata cagcataaga
     1981 gacatttctt tgttaaacaa tgaaataagt gccgcttggg cgcatacgta agctctaaaa
     2041 ttatttgaaa gagaatagta agttttgtcg ccttttggat atttataaaa gagatatcca
     2101 ttgtgaactt gtagcatcaa ggtaacaagg gcaagaaaaa tgaagacgtt attaactctt
     2161 gcaacaacaa aatcattaat ataaataaac aaaaaaacaa gaatcgggac gaaggataat
     2221 ataaaagcaa taattcgcgc tccttttata tcaaatttaa ctacaagcgt ttctatctta
     2281 ttttttttgt caccttcata atccttaaaa taggtataaa acgtcataat tccattcatt
     2341 atccataata aaagcagaat cgatacccta ctttttgttg ccacaacacc aacaggtccg
     2401 gacgcaaaaa agccaaatat tgtgcactga gtaatcatca atccaaatac gatattaccc
     2461 catataccat atgctttcat atactcatat aaaatattga gaatacatcc tgtaaatagt
     2521 ggtatcagtg cgaccggttc gagatataaa agagtaacca cccccattgc tgtcataata
     2581 attgcggata ctaaaacagc aaaaaatgga ttcagtttac cagaaatcat cggacgattt
     2641 ggtgcattta ttctgtcttc tttcatgcca agaaaatcat taacaatttg gtttattccc
     2701 caagcaccaa acagcataat aacaataaca attttcttaa ttgttggtgt aagcacttca
     2761 acggtagcat aatcagatgc gatatgctgg taaaactcca gaccgatcca accggaaatt
     2821 cctgtaacaa aagaatagta gagcctcatt gactttatgt agtaataacc aaaatcaatt
     2881 attttacgca atttacctga taagatatga gccatcatcg tagtatcctg aggtgtatag
     2941 aaccgttttt attttttaga tgcaaataca gagagatttc tgctgtccca tggaatcatg
     3001 aaaaaacaaa aacgctatta atcacagtac ttttatgaaa taatgagaat cactaaagtc
     3061 tcaaaaattc aataattatc gatattaata gcatgtataa agttaaatat tactgtcaca
     3121 aatgcaatca ctaatttgat aaagtagcaa gtgaacaagt gaagtgaaca agtgaacatt
     3181 atggataaaa ggaatacggg ttttaacggt gacggtggtg tttaagagct ttattcgggg
     3241 acgctgacgt tttcagcgat ttgtgtgtaa aatacgggct atattgggga cgaggacggt
     3301 ggtgtttccc tagctaattc aacatccaat aaagattctt aagtggcaac cataaatccg
     3361 gaatgggtgg cagtattgag cggaatacgc aataaccaag tcgcaaagtt tgattgtttt
     3421 caatacactc tttctcaaac tctccacttt gtccaagttt aataaataaa attttatttg
     3481 attcaattct tttcattttc atatgcatgg tcaatagtct ctttaaaatt ttctacgtac
     3541 gagagttgaa gacgttgcgg cggtcgtatc gccgcaatgt gccgaaggcc aagtgattct
     3601 tttcgcaaga gccgcatcaa ccctcagttg tctgccgttg cgttttttag gtcaaagctt
     3661 atcccactct gggactgttc cttctttaat cttacaaatt actgaatcaa aatcttcaaa
     3721 taattcactt ttagtcttct ttttccaatg ttttgaaaat gagttatatg tctccatttc
     3781 ttggatttct tttgtaaatg catataaagc taattgattt atcgacagtc cttgttgatc
     3841 agcaactttt tcaattttat gtttcaattc aattggaatt cttaaagtta atacgcttgc
     3901 tttagtcttc ataatttttt ctccaaatca taacaaactc agctggtgtt acgatagata
     3961 tgtcatcgaa tttcagttca tttgatttta taaaatcttt agtgttactt gtaataataa
     4021 aatcggaatt actggccaat gatagttcaa taaatatatt gtcctcctca tcctttaaat
     4081 tgggtctgaa tgaataataa attgttatag gttttgatat ataggccaga aatcttagaa
     4141 ttttatcaat atcaatagtt gatagtccaa gatccttaat tttatcgggt ctctttaata
     4201 catcttcata ttcagaaaag acaggcaacg aaagagcaag ggttattttg tggttacgga
     4261 taagttgaag aataaaatta gaagccccac tactgcttct cagagcttga tataggacat
     4321 ttgtatcaat taccacaatc atgacttatg atagcacata tactatctat ttgtcaagaa
     4381 attaatcgct ttatagagct aaaacatatg aattcattaa atatcgacaa caattcttgt
     4441 taaggcagag cgtagtaaac catattttga tatgttaaat ataattccaa ccaaatataa
     4501 atgtaaatac aaagatattg gcaaccataa tgatatgaaa agtactacct cgatcaatga
     4561 ccgatcaatt aatatttttt atttctttat tacaggaaaa tatatcaaat acaaatgtag
     4621 taactggaat agcaaaatat attaaacctg ttccaagcaa ttttgcttgg ggaaactcac
     4681 aacgcgtaat aggctttttc agtaatgttt cgcaatttac tttaccaatc cgattagcat
     4741 agcacctatt attaatactt atcataataa acaagaaaaa attcttagct tttataaaag
     4801 agataaaaag ttcttgtagc gtacactcat aaactgtgta tatttataat acagttgtac
     4861 taattacaac aaacagttat ataaactatt attgctattg atgaatgatt ctggagaaaa
     4921 tatgattcgt aataacataa aaaagatagt attgcttttt ataattttcc ctctaattat
     4981 ctttatattc accattttca caaatttgcg tattttgaaa tatggctcac cagtatatac
     5041 aaacattgat acctgccctc aaaaatattg tgcaattgtg cttggagccg gtgtagttca
     5101 gaacatgtat ttaagcccgg tacttgaaga ccgtgtaaaa actgctgttg ctctatataa
     5161 agcaggcaag gtaaaaaagc tacttatgag cggagccaac cataccaaga attataatga
     5221 agttaaggca atgaaaaatc atgctgtcaa atggggagtt aaccctaggg acatatttct
     5281 ggactacgcg ggcttttcta cttacgaatc catgtatcgt gcgcaatata tatttggaat
     5341 caaagaggca gtaataatca ctcaagggtt tcacgtgaat cgctctgttt acctcgccaa
     5401 aaaatttgat attaacgctg ttgggcttgt tgccgaccga cgtagatata tttatataaa
     5461 gcaataccga ttacgtgaaa agcttgcaag agtcaaggct tggattgaat cggtcatatt
     5521 aagacctctt ccgtcttatc ttggtgatcc gataccaatt agcggtgacg gaagagttac
     5581 tcacgacctc tgataaatca gttttcaaaa aattcgaagg cgtcaataat atccagatac
     5641 actccgtcaa atccggcgtc tattatcatt tttagatacg agtcatcgtt tccgtatatt
     5701 atcttttgcc actcttcgtt ccagtactta accttataat ttccttccca atcaggattt
     5761 tcgtcatcca tccaatcagg agaattatta ctccactcat ctttccagta ataacgataa
     5821 tcttcggcct caccgataga catatagcag acaaccatac gtgtagaacc attggctttt
     5881 gttttaagtt cgtctatctg tgatgacgaa tatggttcct gattattgtc aaatagatcc
     5941 atgattacaa gatcataatt agtgttactt acatcattga taaagtcttc tttcgaagag
     6001 tactcttcac cgtttataat atacaaaaag ttttgaacat cgctcattga taaaatatta
     6061 ttgctattct cgttattaat aggatcagga taagtcggaa tagcgttaag atcccgctcg
     6121 tgtgcggcaa aggagatata accggcactg ctgtttaggt cgtaggagtc atcaactttt
     6181 gattgagtac tgcaataatc tgttacaagt atggtattac ccgcatcctt ggaacgatct
     6241 agaaatgctg agataaattg gatatcctca tccggactct ttgcatcgtc ctcatcgtat
     6301 ccgtaaaaaa gatcctcctg accatgtgcg tcaatagcat caagataatc ggtactcaat
     6361 ggaccgtcag tttcgccatc taatgttaca agctcgatac cattttgtgg aattattaca
     6421 aacccaggag agagcttttt tgagtattga cttattgata taacaaagtc tctcatctcc
     6481 tctctgtaat caacatcctc gtcttcattt tcttcatctt ttgatgaatc atcggagcat
     6541 gccgaaacta aaaatatcag aactaaaata agcatttgca taaacttttt catgatttcc
     6601 cccttaatat tgcgaaatca aatctaataa gaacagtaag cacaactgtt gtcagcaata
     6661 gacgtataaa tcatggttta caaaaaaagt aaagaaataa ttgttaacat aatatttcaa
     6721 atggaaattt tcaatgttaa taaacagcaa ttataagtgt tttcgcaagc taccggagag
     6781 ttttaatatc ggcgatcttc ccgaaagttt ttgctatcca caccaatata tgccccaccc
     6841 tgtggccatt gctgctgcca aagatctaat caatttttta caatcatacg agctatttga
     6901 gcatgatttt ggaataaatg agaatcagac aatatctgaa aaaaacggtt taggaaagat
     6961 gtttggcgtt ctgattgtaa aaacatctgt tggcaagcta ggttatttgg caggatactc
     7021 tggaaaaatt ggtgagcgaa acgaccaccc cggatttgtt ccaccaattg ctgaccttat
     7081 ttcgcctggc tcgttttata gaattggaga agaaaaacta aacagaataa acgaaaaaat
     7141 agataagctg caaaatagtt ccactctgtg ctcacttaag aaaaaattgg ctactctgaa
     7201 aacagaatca gaggaacatc tcggtaatca gcgaaaaata ttaaaaacaa aaaagaaaga
     7261 acgagaaatc gaacgaaata taaacaagcc aaaactatcc gacgatgatt acataaaatt
     7321 ttgtgaagag cttaaatcac aaagcatgtc acagcagctt agatacaagg ctgatcgtat
     7381 agattatctt gaacagatac aaaaccttga aaaacagatt gctcctcttg aagaggagat
     7441 acaatcatta aaaacgaaac gtaaaacttt ttctgcccag ttacaacagc aaatatttga
     7501 ctgtttcaat ttcctgaata ttaaaggcga gaaaatgaat attcgggaaa tatttgccaa
     7561 gaccagccaa aaaattgctc catccggagc aggagaatgc gctgctccca aactactcca
     7621 atatgctttc gaaaacaacc ttacaccgat cacaatggca gagttttggt ggggaagatc
     7681 cccacagtca gaaatacgaa aacatggaca ctattatccg gcatgccgtg gaaaatgcgg
     7741 cccgatactt aaacatatgc ttaaaggaat gaaggttgat gataatccgg tagtaccctt
     7801 atctgtatca aattgcgata tcgaaattat ttacgaggat gatctaatcg ctgtggttaa
     7861 caaacctcca gggcttttga ctgtacccgg aaaagataat cacgactcac tctattcaat
     7921 aatgaaggat cgataccccg gagcgactgg tcccctaact gtgcacagac tcgatatgtc
     7981 aaca
//
LOCUS       DAOTGZ010000128         7956 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_3064
            NODE_10941_length_7956_cov_3.851664, whole genome shotgun sequence.
ACCESSION   DAOTGZ010000128 DAOTGZ010000000
VERSION     DAOTGZ010000128.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868088
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 7956)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7956)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:22:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,785
            CDSs (total)                      :: 2,751
            Genes (coding)                    :: 2,732
            CDSs (with protein)               :: 2,732
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 12 of 19
            Pseudo Genes (internal stop)      :: 5 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7956
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10941_length_7956_cov_3.851664"
                     /isolate="AMR_MDS_3064"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1492
                     /locus_tag="P5123_08960"
     CDS             <1..1492
                     /locus_tag="P5123_08960"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRX16431.1"
                     /translation="YQKSSLSHELQYRNDQFYLIPKIKYSQSEYVTHKGWLDDPSSPI
                     NQDMDSGFNVPFIHDGKGTKLMERFFEFEHTTGINAIKGFDFEHNLFFSYNENQFNDY
                     KEISQNHPFKRSKSGESEVRQQFRFPFNFSSTSAENLRNITISYERLSRLYETGVPFE
                     NETESYFNEKYGISRTIMGCSDTSLNLLKYPPWYFFTGRGNFGNGRDHVYDKLNSPLP
                     TGDDPLYDYTNNLYVQDTYDVSSFFSFTGTGASLFSQLTQISERSGIEQLPQQMVQLS
                     NGINLTFDLMQFFSSGFFRPNTGQNARHSAQFFCGYSYSRNMFITSNRLENIHSPSAG
                     IQLGRNRKSISVNTSVDMIFREKEEYISAGTDRDKKDDKYFDNLPEEELKQADKTYTY
                     SCEIKTDIPFLYNLLSRVYKLYDYPMFTIRYDQTLNRYDYKYTVSPEPYDLYMLTQTL
                     ELDVHKNIRGGLTGRYALEKYRDRESGNVTDEILSYEYILGLTVLF"
     gene            1719..2315
                     /locus_tag="P5123_08965"
     CDS             1719..2315
                     /locus_tag="P5123_08965"
                     /inference="COORDINATES: protein motif:HMM:NF015908.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRX16432.1"
                     /translation="MIKSDHSHYGKPLIMLYPGEHIVSSEDIVVSTVAGSCLVVTLND
                     NKRNIGGIAHFILPGQIGTGGITHDDVASYGITQLEYLFGEFVKKGGRREDLTADVFG
                     AAALDVNKKINEAIQRSNINFIHSFFSAEHIPVRTLDLAGSVRRKIVYFPKTGKTYRK
                     MLNHNEDNSEIIRLEHEYIKKAFKELREQTQYIIFNEK"
     gene            2315..2845
                     /locus_tag="P5123_08970"
     CDS             2315..2845
                     /locus_tag="P5123_08970"
                     /inference="COORDINATES: protein motif:HMM:NF015908.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis protein CheD"
                     /protein_id="HRX16433.1"
                     /translation="MLKNYSTKFDKNIITVHPGEYYVSSDDELIQTLLGSCIAVCLHD
                     SSKGISGLNHFMLEGKMLGTNYKEMSTDGKHALHSISQLITDIISAGAKRQDLKAKVF
                     GGGAVLSTEATVHTIPADNIRAAKTILEMEDIPIIQEDLGGKFTRKLVMDVQSGKVFL
                     KKTLGSCDRNSNGYRS"
     gene            complement(3182..3502)
                     /gene="trxA"
                     /locus_tag="P5123_08975"
     CDS             complement(3182..3502)
                     /gene="trxA"
                     /locus_tag="P5123_08975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013430081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0015036 - disulfide oxidoreductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin"
                     /protein_id="HRX16434.1"
                     /translation="MSLLHLNGDNFKSEVEDSDVPVLVDFYADWCGPCRMIAPVIEKL
                     AGKYSGKVKVAKLDVDNASDLAQRFDVSGIPTVILFKGGKPVDRFSGALPENAIDDFI
                     SRNS"
     gene            complement(3534..3815)
                     /locus_tag="P5123_08980"
     CDS             complement(3534..3815)
                     /locus_tag="P5123_08980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012032893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metalloregulator ArsR/SmtB family transcription
                     factor"
                     /protein_id="HRX16435.1"
                     /translation="MKNKCDVDYDYYAELLKAIGHPVRLQIVAGILCDECNVNGIVEK
                     LNIPQSTVSQHLGVLRSRGIITAHKDGVSTCYKVSNEFILHIITLMKKS"
     gene            complement(3876..4916)
                     /locus_tag="P5123_08985"
     CDS             complement(3876..4916)
                     /locus_tag="P5123_08985"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005991016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HRX16436.1"
                     /translation="MANEKVKTEKSGKRKKSTQVEVSVILPVYNEEDNIVRQYEKLTS
                     SMHKIGKKYEIIFVDDGSVDKSVELLSEIAQRDKSVKLVAFRRNFGQTAAMRAGIDHA
                     AGDIIVFMDSDLQNESDDIELLLDKIEEGYDVAAGWRKKRHDKFFTRKVPSFFANHII
                     AWVTGVKLHDLGCSLKAFRADVLKKVKLYGEMHRFIPVHASWIGASIIEVPVRHHARK
                     YGKSKYGMMRTFKVLLDLITIKFLGSFSTKPIYLYGGLGIATMFLAAISAVLVITMKI
                     FWRMNITGNPFFQLFFLFVILSVILALMGVQTEILIRIYHESTHRSPYYVKDVVDGPG
                     YVKKSVKKVKKS"
     gene            complement(4936..6918)
                     /locus_tag="P5123_08990"
     CDS             complement(4936..6918)
                     /locus_tag="P5123_08990"
                     /EC_number="3.4.21.102"
                     /inference="COORDINATES: protein motif:HMM:TIGR00225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxy terminal-processing peptidase"
                     /protein_id="HRX16437.1"
                     /translation="MKRLTRKKALFVVTLLVLLIGCFKKSDGLDGSDIQSIMRSILYR
                     HVSQNEFTDEVSRRMLENLIKLTDPYKLYFYESDIDSFRKHADKLNDYASSGDLSFIT
                     SYFKLFKSRYNESMEIFSKRVDGEFDFTKDEYMIVDREKIEFAGSKKELEERWRKRIK
                     FQLLNYLNMDMTMDESRKRLKRHYDMVGKEMMKFDQEKVISIYLNAFSSALDPHSAYM
                     TAKDYEDFKISMNLKLEGIGAVLRSEDGFTYVDSVIPGGPVSKMDPDKTVQPNDKIIA
                     VAQGEKEPESIIDMPLREAVDKIRGKKGSIVRLVIMRKAPGEKGEKRVIVSVERDQVV
                     LEQQAAKHAVHTVKKEKSSVKVGYIKLPTFYVDWEEYYKRNPEAKMSARDVFNSIHAL
                     NKEKIDVMVFDLRNNPGGGLEQAELIAGYFLPGGPVLQVKDHEQVQVRYDKDPAVYYD
                     GPLVVLVNNQSASASEIFAGTIRDYKRGLIVAPTRTFGKGSVQQLTDLRQGGAVKITI
                     QLFYQPSGTSNNKDGIVPHITVPSYSQLINYYESDLDYALDWTPIKPAPYKDFGNTFF
                     TKGLINRLSNLSGARVKSDPDFKEMQKKIAEYLKEDNTKKISLKMDPSGKDDKVGEEA
                     SVSKPDPDAVYDLKNDIFLREAFNIAADYAEARKGK"
     gene            7288..>7956
                     /locus_tag="P5123_08995"
     CDS             7288..>7956
                     /locus_tag="P5123_08995"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRX16438.1"
                     /translation="MAKILFISDLNFGSKSELPVTDEHRLLTLEKICSIGVHHDILLI
                     GGNLFGTNKVDNVTINKIAAQIELLNKENTEVYITAGEYELENCEKLEESGLIILEHG
                     TVHKGITGIGVSGFQIKSGESLDLITQPQTESPVIALINATTSIETELKKVLAEKNDS
                     GFAFFAIGGNHDFKIFRVAGKPVGAQCGSSEPLKRSCKGARYALSFEISDQSPEKVSR
                     YQVNT"
BASE COUNT         2482 a         1709 c         1386 g         2379 t
ORIGIN      
        1 ctatcaaaaa agtagtcttt cacacgagtt acaataccgt aatgatcaat tttatctcat
       61 acctaagata aagtacagcc aatccgaata cgtgactcac aaaggatggt tggatgaccc
      121 ttcatctcca attaatcaag atatggatag tggttttaac gttcccttta ttcatgacgg
      181 caaaggaaca aaactaatgg aacgtttttt tgaatttgag cacacaaccg gaataaacgc
      241 tatcaagggt ttcgatttcg agcataatct ttttttcagc tataatgaaa atcagttcaa
      301 tgactacaaa gagatcagcc aaaaccatcc gtttaaaaga tccaaaagcg gtgaatctga
      361 ggttagacag cagttcaggt tcccttttaa cttttcatcc acctcggctg aaaacctgcg
      421 aaacataact ataagctatg aacgtctgtc acggctatac gaaactggag taccattcga
      481 aaatgagaca gaaagctatt tcaatgagaa atacggaatt tcaagaacca ttatgggatg
      541 ttctgataca tcattgaatc ttctcaaata tcccccttgg tactttttca cagggcgcgg
      601 aaacttcggc aatggtcgag atcatgttta cgacaaactc aactcaccat tgcctactgg
      661 tgatgatccc ttgtatgact acacaaataa tctatacgta caggatacct atgatgtaag
      721 ctcgtttttc agcttcactg gaaccggagc ctctcttttc agccaattaa cgcaaataag
      781 cgaaagatcc gggatcgaac agctgccgca acagatggta cagctttcaa atggcatcaa
      841 cctaaccttt gacctgatgc agtttttcag tagtggtttt tttagaccca acacaggaca
      901 aaacgcaaga cactcggctc agtttttttg cggatacagc tattcaagaa acatgtttat
      961 tacatcaaac cgattagaaa acatccattc tccatctgcc ggaatacaac tcggtcgcaa
     1021 ccgcaaaagc atatcagtaa acacttctgt cgacatgata tttcgtgaaa aagaagagta
     1081 cataagcgcc ggaacagata gggacaaaaa ggatgataag tattttgaca atcttcccga
     1141 agaagagtta aaacaagcag ataaaaccta tacctatagc tgtgaaataa agacagatat
     1201 tccatttctg tataacctgc tatctagagt atataagctt tacgattacc ccatgtttac
     1261 aatacggtat gaccagactt taaatcgtta tgattacaaa tatacagtat ctcccgagcc
     1321 ctacgatctt tatatgctaa cacaaaccct tgaacttgat gtacacaaaa atattcgagg
     1381 cggtcttacc ggacgatacg ctcttgagaa atacagagat agggaatctg gtaacgtaac
     1441 agacgaaata ctctcttacg agtatattct gggtttgacc gtactctttt aaacactacc
     1501 tatcatccaa ttccaatttt ttgttttatg accgtttttt ttgctaattt ggatgttttt
     1561 tcttaaaatg tttattatta ttaattgtat aagtgcaagg catagcaagc tttctcaaac
     1621 ttccatatac gaacttttaa aaacggctct gaaaagatac aaccgaattt tacgagtttc
     1681 agtatatttg ttcatcagat agatagatag aggtctacat gattaaatcc gatcactccc
     1741 attacggcaa accgcttatc atgctttatc cgggagaaca cattgtttct tcagaagata
     1801 ttgtagtatc aaccgttgct ggcagctgtc ttgtggttac cctcaatgat aataaaagaa
     1861 acataggcgg catcgcccat tttatactgc ctgggcaaat tggcacaggt ggcataacac
     1921 acgatgatgt tgcgtcatat ggtataacac agctagagta cctgttcgga gagtttgtaa
     1981 agaaaggtgg ccggcgggaa gatctaacag cggacgtctt tggggcagcc gctcttgatg
     2041 taaataaaaa gattaacgaa gcgattcagc gaagtaatat aaattttatt catagctttt
     2101 tttccgctga gcatataccg gtacgcactc tggatctggc aggcagcgta aggcgcaaaa
     2161 ttgtatattt ccctaaaacc ggcaaaacat acagaaaaat gcttaaccac aatgaggata
     2221 actcggagat tattaggctt gaacatgagt atataaagaa agcatttaaa gagctcaggg
     2281 aacagactca atatattata tttaacgaga agtaatgtta aaaaattatt caacaaaatt
     2341 tgataaaaac ataatcacag ttcatcccgg tgaatattat gtctcttctg atgatgaact
     2401 tattcaaacc cttcttgggt catgcatagc ggtgtgcctt catgactcgt caaaaggaat
     2461 atcaggtctt aaccatttta tgcttgaagg taaaatgttg ggaacaaatt ataaagagat
     2521 gagtactgac ggtaaacacg ctctccattc catttctcag ctgattaccg atataatatc
     2581 ggccggagcc aaacgacaag atcttaaggc aaaagttttc ggtggaggtg ctgttctttc
     2641 gaccgaagca accgtacaca cgataccggc agataatatc agggctgcca aaacaattct
     2701 ggagatggaa gatataccga tcattcagga agatcttgga ggcaagttta ccagaaaact
     2761 tgttatggat gttcaaagtg gtaaggtttt tttaaaaaaa acgctcggca gttgcgacag
     2821 aaacagcaac ggttatcgaa gctgacgttt aacggttagc aaattcaatg cgtaattgaa
     2881 catcctgtcc gcaaaggctt tgcggttaac agtgtgacca cccacctaaa tcaagaccga
     2941 gcatgtaatg taaaataaac agcttttagg ctggtctttc ttccttccgt ccggatagat
     3001 agagaaaaat actaaaaaaa tgctgttgat tcaatttata ttgaggaagg ttctgattaa
     3061 aaagttacct gtaaaaattt gaaaacttta taaactgctg caaacaaccc atttatgagc
     3121 agatgcagca gttataaata tatttcattt tctttgtcaa tttccggtta acagcattat
     3181 ttcagctgtt tcgggaaata aaatcatcaa tcgcgttttc aggcaaagca ccactaaacc
     3241 tgtctacagg ttttccacct ttaaacaaaa ttactgttgg tattccgctc acgtcaaaac
     3301 gctgtgccag atccgaagca ttatcaacat caagtttcgc cacctttacc ttaccgctgt
     3361 acttgccagc aagtttttcg ataactggtg ctatcatacg acacggacca caccaatcag
     3421 cataaaaatc caccaaaaca ggcacgtccg aatcttccac ttcagatttg aaattatctc
     3481 cattaagatg caataaactc atgtaacctc ccgctgaagc agaattaccc tgcttagctt
     3541 tttttcatta atgtaattat gtgcaaaata aactcattac tgaccttata acaggtactt
     3601 actccatcct tatgagcagt aataataccc ctactccgca aaactcccaa atgctgagaa
     3661 actgtagact gaggaatatt cagtttttcc acaattccgt taacattgca ctcatcgcaa
     3721 agtatgccgg caactatctg aagcctcacc ggatgtccta tcgcttttaa aagctccgca
     3781 taatagtcgt aatcaacgtc acatttattc ttcataaaag caccttaata atatcgtatt
     3841 attgcaatat tttgtcaata cattttaagt aaaaatcagc ttttttttac tttttttacg
     3901 cttttcttca catatcctgg cccatcaacc acgtccttaa cataatatgg cgacctgtga
     3961 gtggattcat gatatattct tatcaaaatt tctgtctgta cacccattaa agcaagaata
     4021 acactcaata ttacaaaaag aaaaaatagc tgaaagaatg gatttccagt aatattcata
     4081 cgccagaata ttttcatcgt tataaccagt actgcagaga tagccgctaa aaacatggtc
     4141 gctattccca aaccaccata tagatatatc ggtttggtag agaaagaacc caaaaatttt
     4201 atagtaataa gatcaagcaa aaccttgaaa gtacgcatca tgccatactt tgattttccg
     4261 tattttcgtg catgatgtcg taccggaacc tcaataatcg atgccccaat ccacgaagca
     4321 tgtacaggaa taaaccgatg catttcgcca tatagtttaa ctttttttaa tacatctgcc
     4381 cgaaaagcct ttagagaaca gcccaggtca tgtaatttga caccggtaac ccaggcaata
     4441 atatgattcg cgaaaaaaga tggtactttt cgagtaaaaa atttatcatg cctttttttc
     4501 cgccaaccgg ccgccacgtc gtacccctct tctatcttat ccaaaagaag ctcaatatca
     4561 tctgactcgt tttgaagatc agaatccata aagacaataa tatcaccagc agcatggtca
     4621 atcccggcac gcattgctgc ggtttgacca aaatttctgc gaaacgctac cagtttaaca
     4681 ctcttatctc tttgagctat ttctgacaac aattcaaccg acttatcaac acttccgtca
     4741 tccacaaaaa taatttcata tttcttacca attttatgca tgctggatgt caatttttca
     4801 tactgccgaa caatattatc ttcctcgttg taaacaggaa gaataaccga aacctctact
     4861 tgtgtcgatt tttttctctt gccacttttt tccgttttca ctttttcgtt agccattatt
     4921 aattatcctc cggaattact tgccctttct ggcttccgcg taatcggcag caatattaaa
     4981 tgcttccctt agaaatatgt cattttttag gtcgtacacc gcgtctggat ccggcttgct
     5041 aacactggcc tcttcaccga ccttatcatc ctttcccgac ggatccatct taagactgat
     5101 ctttttggta ttatcttctt taagatactc agctatcttt ttttgcatct ctttgaaatc
     5161 tggatcactt ttaactcggg ctccagataa gtttgagagc cggtttatta accctttggt
     5221 gaaaaaggtg ttgccgaaat ccttgtaagg agcgggtttt atgggtgtcc agtcaagagc
     5281 atagtccaga tcgctctcgt aataattaat cagctgactg tacgaaggaa ccgtaatatg
     5341 tggaactatt ccatccttgt tatttgaagt acccgaaggc tgatagaaaa gctgtattgt
     5401 aatctttaca gcgccccctt gacgaagatc cgtgagttgt tgaacacttc ctttaccaaa
     5461 agtacgggtc ggagcaacga taagtcctct tttataatcc ctgattgtac ctgcgaatat
     5521 ttcagaagcg ctggcactct gattatttac caaaacaacc agcggaccat cataatagac
     5581 ggcaggatct ttatcatagc gcacctgcac ctgttcgtga tccttaacct gtaataccgg
     5641 cccaccagga agaaaatacc cagcgattag ttcggcctgt tctagaccac ccccagggtt
     5701 attccgcaaa tcaaaaacca taacatctat tttttcttta tttaaagcat gtatcgagtt
     5761 aaatacatcg cgagcagaca tcttcgcttc aggattccgt ttatagtact cttcccaatc
     5821 gacgtaaaaa gtaggcagct ttatataccc gaccttaaca ctactcttct ctttttttac
     5881 agtatgtaca gcatgcttgg ctgcttgctg ctctaaaacc acctgatctc gctctaccga
     5941 tacaattact cttttttcac ccttttcacc gggtgctttt ctcattatta ccagtcgcac
     6001 gatagatccc ttcttaccgc gaatcttatc aaccgcttca cgcagaggca tatctatgat
     6061 actttcgggt tccttttctc cctgtgctac agcaattatt ttgtcgtttg gctgcacggt
     6121 tttgtcggga tccatcttgg aaacaggtcc tcctggaatt accgaatcaa catatgtaaa
     6181 cccgtcttca gaccgaagca ctgcaccgat tccttcaagc ttcaagttca ttgatatttt
     6241 aaagtcctca taatccttgg cagtcatata cgcagaatgg ggatcaagag ctgaactgaa
     6301 tgcattaaga taaattgaaa ttactttttc ctgatcgaat ttcatcatct ctttgccaac
     6361 catgtcataa tgacgtttga gtctttttcg cgactcatcc atggtcatat ccatatttag
     6421 gtagttaagg agctggaatt tgattctttt tctccatctc tcttccagct ctttttttga
     6481 accagcgaat tctatttttt cacggtcaac gatcatatac tcatctttgg taaaatcgaa
     6541 ttcaccatca acccttttac tgaatatctc catactttcg ttatatcgtg atttaaacag
     6601 tttaaaatag cttgtgataa acgaaaggtc acccgatgag gcatagtcat tcagtttatc
     6661 agcatgtttg cggaacgaat ctatgtcaga ttcatagaaa tagagcttgt acggatcagt
     6721 gagtttaatc agattctcga gcattcttcg tgaaacttca tctgtaaact cgttctgcga
     6781 tacatgacgg tataatatcg atctcataat cgattgtata tccgaaccat caagcccgtc
     6841 agatttttta aaacatccga ttaacaaaac aagcagagtt acaacaaaga gcgccttttt
     6901 gcgggttaat cttttcataa tagataatta tcctgattat ttgtcttttt taaggattct
     6961 tccttaactg gccttaaggg atagcagtat tgcttaaagt tacaaaatat ctgcgacaag
     7021 taaacccagg tcacatctcg gaagagataa caatatagca ttgagtttaa gcattgaagt
     7081 caacaaaaaa ttgccatatt ttaataatct cgatttttac gactttgcga aaccggaagt
     7141 tttgctattc tatttttatt aagaacaata tcaagattac cgatacttaa gagattcata
     7201 actttcttga cggctttctc tgaaacatgg ataaagttaa tatattaatc ttcttcaaac
     7261 aaatcaacag gagcagatat ccgaaaaatg gcaaaaatcc tttttatatc cgatcttaac
     7321 ttcggttcaa aatccgaact gcctgtaacg gatgagcatc gtctgcttac tctcgagaaa
     7381 atatgctcca ttggagtaca tcatgatata ctgctaatag gcggcaattt attcggcaca
     7441 aataaggttg ataatgtcac aatcaacaaa attgctgcac agatagaact tctaaacaaa
     7501 gagaatacgg aagtatacat caccgcaggg gaatatgaac ttgaaaactg tgaaaaactt
     7561 gaagaaagcg ggcttattat cctcgagcac ggaacagtcc acaagggtat aaccggaatt
     7621 ggggtatccg gctttcaaat aaaatctggc gaaagcctgg atcttataac ccagccgcaa
     7681 accgaatccc ctgttatagc tctgatcaat gcaactactt caattgaaac agagctaaag
     7741 aaagttttgg ccgaaaaaaa cgattctggt tttgcttttt tcgctatcgg cgggaatcat
     7801 gattttaaaa tatttcgtgt tgccggtaaa cccgtaggtg ctcaatgcgg ttcaagcgaa
     7861 cctttgaaac gatcatgcaa gggtgctcgt tacgctttat cttttgaaat tagcgaccag
     7921 agccccgaaa aagtatcccg ttatcaggtt aataca
//
LOCUS       DAOTGZ010000129         7940 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_3064
            NODE_10965_length_7940_cov_5.198224, whole genome shotgun sequence.
ACCESSION   DAOTGZ010000129 DAOTGZ010000000
VERSION     DAOTGZ010000129.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868088
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 7940)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7940)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:22:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,785
            CDSs (total)                      :: 2,751
            Genes (coding)                    :: 2,732
            CDSs (with protein)               :: 2,732
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 12 of 19
            Pseudo Genes (internal stop)      :: 5 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7940
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10965_length_7940_cov_5.198224"
                     /isolate="AMR_MDS_3064"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..433
                     /gene="radC"
                     /locus_tag="P5123_09000"
     CDS             <1..433
                     /gene="radC"
                     /locus_tag="P5123_09000"
                     /inference="COORDINATES: protein motif:HMM:TIGR00608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="DNA repair protein RadC"
                     /protein_id="HRX16439.1"
                     /translation="ILSALEMGRRIVSREIETQELNNPERVWRNVLSDITGLDHEIFI
                     VIVLNSKNRILKKSIVSCGTVSETIVHPREVFRAAIKENGSSIIICHNHPSGNLEPSK
                     NDINITERLVKASKIIGIDLLDHLIVTEESYCSLRETGYID"
     gene            complement(498..1748)
                     /gene="lysA"
                     /locus_tag="P5123_09005"
     CDS             complement(498..1748)
                     /gene="lysA"
                     /locus_tag="P5123_09005"
                     /EC_number="4.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011306632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008836 - diaminopimelate decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopimelate decarboxylase"
                     /protein_id="HRX16440.1"
                     /translation="MMSKTLPFNKQEIEEIIKKHPTPFHIYDAEAIKNNAIKLNEIFG
                     SFYGFKEFFAVKATPNNHILALLKSCGLGADCSSLPELILAEKAGIVGEEIMFSSNNT
                     PASEYRRAIELGAIINLDDISHISFLEEHASLPELICFRYNPGPLRDGNSIIGSPEES
                     KYGFTKDQLFEGYSLLKQKGVKRFGLHTMIASNELNEVYFAETAKMLLELCVELKNEL
                     DINIEFINIGGGIGIPYRPEDKNVNLEHIASSIKDLYRKFITDNSMNPVKLFMENGRM
                     ITGPYGYLVTRAIHKKEIYRNYIGIDACMANLMRPALYGAYHHITVMGREGEPKDHVY
                     DIAGSLCENNDKFAIERPLPRIQTGDIVVIHDTGAHGHAMGFNYNGKLRSAELLMQKD
                     KSVKLIRRAETLDDYFATLDFDSN"
     gene            complement(1768..2256)
                     /gene="lspA"
                     /locus_tag="P5123_09010"
     CDS             complement(1768..2256)
                     /gene="lspA"
                     /locus_tag="P5123_09010"
                     /EC_number="3.4.23.36"
                     /inference="COORDINATES: protein motif:HMM:TIGR00077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0004190 - aspartic-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase II"
                     /protein_id="HRX16441.1"
                     /translation="MKNHVYALIIFAVVLCADFITKHFVDLNFLEGERIKFLGGFFQI
                     IKIYNRGGIFGIAQGYQFIFLMLSIVVFFVLLAFYIIETVHHRLSNSLFSLSMGLVFS
                     GAIGNILDRALGKPGVVDFIYIGFEDFFMWPAFNIADMAIVTGALGLTIFFMISGQKN
                     FQ"
     gene            complement(2297..5065)
                     /gene="ileS"
                     /locus_tag="P5123_09015"
     CDS             complement(2297..5065)
                     /gene="ileS"
                     /locus_tag="P5123_09015"
                     /EC_number="6.1.1.5"
                     /inference="COORDINATES: protein motif:HMM:TIGR00392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004822 - isoleucine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006428 - isoleucyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isoleucine--tRNA ligase"
                     /protein_id="HRX16442.1"
                     /translation="MDYSKTVNLPETDFPMRANLPQREPEILKKWAKEDIYNKILSER
                     SNNKLFILHDGPPYANGHIHMGHALNKILKDVIIKHKTMMGFKTPYIPGWDCHGLPIE
                     LKVTQKLGDKARDMEKIAIRNLCRDYATEFINIQRDEFKRLGVFADYKNPYKTMTKDY
                     EATITQKFAELFEQGYIFKGKKPIYWCPSCETALAEAEVEYEMHTSKSIFVKFAIDAS
                     SIDLEGVNSDKCHVVIWTTTPWTLPANLAVCFHPDFPYSLYKFKEEFLLLADGLIEQF
                     ESVMGKKHSESFSISRSQIEKLVVHHPFIKRESKVIFGHHVTLEQGTGIVHTAPGHGA
                     EDYHIGIQHGLDVFCPVDHQGRFTDEYSDMKGVLVWDANDKVIEIIKGNNTLIEQNQI
                     EHSYPHCWRCKKPLIFRATEQWFLGIDTHDLRKKASKVVQDTNWVPSWGESRFRSMVE
                     QRPDWCLSRQRSWGVPIPSFSCTECGANMTSTESLMHFSSLAKSEGIDIWYTKDINEL
                     IPEGTKCKCGSTSFVKENDILDVWFDSGVSHFAVLESPYREGLRWPADLYLEGSDQHR
                     GWFQSSLWPALALKGRAPYDTVLTHGFILDENGKAMSKSMGNVIPPEDIIKKYGADIL
                     RLWVTSEDYRNDLKIGHDMLKQVADSYRKIRNSFKFLIGNLSDFNEEKDTVPYEELAD
                     MDKWILFKLYQLQQQVINSYEKFEFHLVYRRILNFCAVELSSLYFDVSKDILYIEQRN
                     AKKRRSTQTAINEIFKTLLTLSAPVLAFTSEEIWDFIGKEGSIHTQNYYSLPEEYNNS
                     GIEQSFGVIVGVKKDLLKALERARNEKIISKSQEASVTIVMNSDDDKNIADAKAEDMQ
                     GFLQVAKITLAQDTDSKFYEMDSCKIKIERFDGKKCVRCWNYFENLGEDADHPELCSR
                     CTAVIKSC"
     gene            5297..5818
                     /locus_tag="P5123_09020"
     CDS             5297..5818
                     /locus_tag="P5123_09020"
                     /inference="COORDINATES: protein motif:HMM:NF012797.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA methyltransferase"
                     /protein_id="HRX16443.1"
                     /translation="MRKLRHDEIVAFQKIQSDKNRRFPVSAVLENIRSLHNVGSMFRT
                     ADGAGLNKLYLCGFTGAPPRPEIEKSALGSTDYVNWERQEETLTLVRQLKSMGEQIVV
                     FEHCEQAKSYNRIEYRYPCVLVFGNEVNGVSSEVCALADETVLIPMYGSKQSLNVSVS
                     FGIGVYNAIASLS"
     gene            complement(5868..6761)
                     /locus_tag="P5123_09025"
     CDS             complement(5868..6761)
                     /locus_tag="P5123_09025"
                     /EC_number="1.8.4.11"
                     /EC_number="1.8.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010259865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional methionine sulfoxide reductase B/A
                     protein"
                     /protein_id="HRX16444.1"
                     /translation="MKKLTESEKYVIIEKGTEPPFTGEYTDLNEPGIYVCKQCDNPLY
                     QSTAKFKSGCGWPSFDDQVEGAVQEIADKDGMRSEIVCANCGGHLGHIFKGEGFTSKN
                     VRHCVNSISMKFIPLKEISDKTLAITKEKISDSSKNYERAIFAGGCFWGIEYHFLNIE
                     GIVSTMPGYTGGKTDKPTYEEVCSGKTGHVEAVEIIYDPSKISYSKLAELFFEIHDPS
                     QKDGQGPDIGEQYKSVIFYTDYKQKVVAETLINTLRNNGTDAITSILPAKDFYPAEDY
                     HRRYYEKNGKFPYCHQRTKRF"
     gene            complement(7039..7599)
                     /locus_tag="P5123_09030"
     CDS             complement(7039..7599)
                     /locus_tag="P5123_09030"
                     /inference="COORDINATES: protein motif:HMM:NF013868.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043856 - anti-sigma factor antagonist
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="STAS domain-containing protein"
                     /protein_id="HRX16445.1"
                     /translation="MSAITNEHIITIDIEGKLNGETAVALEANLNSLIANGKKRVILY
                     CKQMTFVTSYGISFLLLWSSKMNKEGGTLVLCGINREITSLFSILKIAKFIAVYPDKN
                     QAIEAIKGSLFKQSPAPSYRDEDLVRSEEPVINIDSQNILDFPSPLVIECATCGALVR
                     VYRPGDYICPGCKTEFHIKQDGTAIF"
     gene            complement(7604..>7940)
                     /locus_tag="P5123_09035"
     CDS             complement(7604..>7940)
                     /locus_tag="P5123_09035"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRX16446.1"
                     /translation="SDNGEGVIAIMHSKKSLYSFINKQLTRLTNIKYIIFAMALIIAS
                     LIAILLYTVLRCKQTNSSNIICESALQPDLSPAPNVNNSNFYNDVRPLQSGVEPVILD
                     AIPIKKRGN"
BASE COUNT         2312 a         1457 c         1535 g         2636 t
ORIGIN      
        1 tattctttcg gcacttgaaa tgggacgtcg cattgtatca agagagattg agactcaaga
       61 gttgaataat ccggagcgtg tatggagaaa tgtactttct gatattactg ggcttgatca
      121 cgaaatattt atagttatag ttctaaacag taaaaacagg atacttaaaa aaagtattgt
      181 tagttgtgga actgtgtctg aaacaatagt gcatcccaga gaagttttta gagctgcaat
      241 aaaagaaaac gggtcatcaa tcattatatg tcataatcat ccgtctggaa atcttgagcc
      301 gtctaaaaat gacataaaca ttaccgagag acttgttaaa gcatccaaaa ttattggaat
      361 tgatcttctt gaccatctta ttgttacgga agagagctat tgtagtcttc gtgaaaccgg
      421 ttatatcgat tagtataacc ggttctactg ataatagcag agaacttaaa aataaaccaa
      481 tatgttaaat gaaaagttta gttggagtca aaatccaaag tagcgaaata gtcatccaga
      541 gtttcagctc tgcgaataag ctttacgctt ttatcttttt gcattagtag ctctgctgaa
      601 cgaagttttc cgttgtaatt aaagcccata gcatgcccat gagcccctgt atcgtgtatt
      661 acaacaatat cgcctgtttg aattcgtgga agagggcgtt caattgcaaa tttatcattg
      721 ttttcgcata gtgatccggc aatatcgtaa acgtggtcct ttggctcacc ttcgcgtccc
      781 ataacggtaa tatggtggta tgctccgtat agtgcaggtc gcatcagatt ggccatgcaa
      841 gcgtcaatac caatgtaatt gcggtatatt tctttcttgt gtattgctcg tgtaactagg
      901 taaccgtatg gcccagtaat cattcttccg ttttccataa aaagtttaac gggattcata
      961 gagttgtcag tgataaactt acggtaaaga tccttgattg atgaggcgat atgttcaaga
     1021 ttaacgttct tgtcttcagg tctatatggt atgcctatgc ccccaccaat attaataaat
     1081 tcaatattaa tatccaattc atttttaagt tctacacata gctcaagaag catttttgca
     1141 gtttcagcaa aatatacctc gtttaactcg ttagatgcaa tcatggtgtg aagaccaaaa
     1201 cgtttaactc ctttttgttt aagcaaacta tagccttcaa aaagctggtc ttttgtaaag
     1261 ccgtattttg attcctcggg agaaccaatt atagagttgc cgtctcttag tggtccaggg
     1321 ttatatctga agcagataag ttctggaagt gatgcatgtt cttcaagaaa agaaatatgt
     1381 gagatatcgt caagatttat tattgctcca agttcaatag cgcgtctata ttcagaggca
     1441 ggggtattat tagatgagaa cataatttct tcgcccacaa taccggcttt ttctgcaaga
     1501 atcaactcgg gcagagagct gcaatctgct ccaagaccac atgattttag aagcgcaaga
     1561 atatgattgt taggagtggc ttttactgca aaaaattctt taaaaccgta aaaagaacca
     1621 aaaatttcgt tcaatttaat tgcattgttt tttattgctt cggcatcgta tatatgaaaa
     1681 ggggtggggt gctttttgat gatttcttct atttcttgtt tattgaaagg gagtgttttg
     1741 ctcattatta attttcctgt tgttaattta ttgaaagttt ttttgtccgc taatcatgaa
     1801 aaaaatggta agcccaagag ctcccgttac aatggccata tctgcaatat tgaaggcggg
     1861 ccacataaaa aaatcttcaa atcctatgta gataaaatcg acaacacctg gttttccgag
     1921 agcacgatcc agaatgttcc caattgctcc ggaaaagaca agacccattg aaaggctgaa
     1981 aagggaattt gaaaggcggt ggtgtactgt ttcgataata taaaaagcaa gaagtacaaa
     2041 aaaaaccacg atcgaaagca ttagaaaaat aaattgatat ccttgagcaa taccaaaaat
     2101 accacctcga ttgtaaattt ttataatttg gaaaaaacca cccaagaatt ttattcgctc
     2161 gccttcaaga aaattcagat caacaaaatg ctttgtgata aaatctgcac aaagtacaac
     2221 cgcaaaaatt atcaacgcgt agacgtgatt tttcatcatc tttctccgaa gtatggtttt
     2281 tccaaaacta tatctcttaa cagcttttaa ttacagctgt acatcttgaa caaagttcag
     2341 gatgatcggc gtcttctcca agattttcaa agtagttcca acatctgaca cattttttac
     2401 catcaaatct ttcaattttt atcttacagc tatccatttc gtaaaattta ctgtctgtat
     2461 cttgagcaag agttatcttt gcaacctgta ggaagccttg catatcttca gcttttgcgt
     2521 cagcaatatt cttgtcgtca tccgagttca ttacaatagt aactgatgct tcttgagatt
     2581 tggatattat tttttcgtta cgagcacgtt ctagtgcttt gagaagatct tttttgactc
     2641 ccacaatgac accaaaactc tgctctattc cgctattatt atattcttcg ggtaatgaat
     2701 agtagttttg tgtatgaatg cttccttctt tgccgataaa gtcccatatt tcttcgctag
     2761 taaaagctaa aacaggagcc gaaagggtaa gcaatgtttt aaatatttcg tttattgctg
     2821 tttgagtaga ccttcgtttc tttgcattac gttgttcgat gtaaagaata tcttttgata
     2881 catcgaaata tagagatgat aactcaacag cgcaaaagtt gagtatgcgt ctgtatacaa
     2941 gatgaaactc gaatttttcg tatgaattaa ttacttgctg ttgaagttgg tagagtttga
     3001 acaaaatcca tttatccata tctgctagct cttcgtaggg tactgtatct ttttcttcat
     3061 tgaagtcgga aaggtttcca ataagaaatt tgaatgagtt acgtattttt ctgtaagagt
     3121 cggcaacctg tttgagcatg tcgtgaccta ttttaagatc attacgataa tcttcggatg
     3181 tcacccagag tctaagtata tccgcgccat atttctttat aatatcttca ggcggtatta
     3241 cattgcccat tgatttactc atcgctttac cgttttcatc cagtatgaat ccatgagtca
     3301 aaacggtatc gtatggagct cttcctttta aagcaagggc aggccataga gatgattgaa
     3361 accagccacg atgttgatca cttccctcaa gatagaggtc agccggccaa cgtaagcctt
     3421 cacggtaagg gctctctaaa acggcaaagt gtgagactcc ggaatcgaac cacacatcaa
     3481 gtatgtcgtt ttcttttaca aaggaggtgc ttccgcattt acattttgtt ccttcgggta
     3541 ttagctcgtt aatatctttg gtataccata tatcaatacc ttccgattta gcaagtgacg
     3601 aaaaatgcat aagtgattcg gtagaagtca tgtttgctcc acattctgtg caagagaatg
     3661 aaggtattgg caccccccac gaacgttggc gtgacaaaca ccagtcaggg cgctgttcta
     3721 ccattgaacg gaaccttgat tcgccccatg agggaaccca gtttgtgtct tgaactacct
     3781 tggaagcttt ttttctaaga tcatgagtgt ctattcccag aaaccactgt tcggttgctc
     3841 taaagatcag aggtttttta caacgccagc agtgagggta tgagtgttct atctgattct
     3901 gctctataag tgtattgttg cctttgatta tttcgataac cttgtcattt gcatcccaga
     3961 caagaacacc tttcatatca gaatactcat cagtgaatcg tccttggtgg tctacaggac
     4021 agaaaacgtc cagtccgtgt tgtatgccaa tatgatagtc ttccgcgcca tgtccaggtg
     4081 cagtgtgaac aataccagta ccctgttcaa gagtaacatg atgcccaaag ataaccttag
     4141 attcgcgttt tataaaaggg tggtgtacaa caagtttttc tatttgagat ctgctgatac
     4201 tgaaagattc agaatgtttt ttacccataa cagattcaaa ctgttcaatt aatccatcgg
     4261 ccagtaaaag aaactcctct ttgaacttgt aaagtgaata tgggaagtca ggatgaaagc
     4321 atacggcaag gtttgccggc aaagtccatg gtgtagttgt ccatattact acatggcact
     4381 tatctgagtt aactccttct aggtcgatac tggaagcatc aattgcaaat ttaacgaaga
     4441 tggatttgga agtgtgcatc tcgtattcta cttccgcctc ggcaagagca gtttcgcagc
     4501 ttgggcacca gtagattggt ttttttcctt taaaaatata gccctgttca aaaagttcag
     4561 caaatttctg tgttattgtt gcttcgtaat ccttggtcat tgtcttgtat ggatttttat
     4621 aatctgcaaa aacaccaagt cgtttgaatt cgtcccgctg gatgtttata aattcggttg
     4681 cgtaatcacg gcaaagatta cggattgcaa tcttttccat atcgcgggcc ttatccccca
     4741 atttttgggt taccttgagt tcgataggaa gaccatggca atcccaacca ggaatatagg
     4801 gagttttaaa ccccatcata gttttatgtt tgattattac gtcctttaat attttattga
     4861 gggcgtgccc catatgaatg tgtccgtttg cataaggagg gccatcgtgc agaatgaaga
     4921 gcttattatt ggatcgttcg gaaagaattt tgttataaat atcttctttc gcccattttt
     4981 ttaatatttc cggttcacgt tgaggcaggt tcgcgcgcat cggaaagtcg gtttcgggta
     5041 aatttacggt ttttgaataa tccatatgag cctcacattc tgttaaagtt tgcattaaaa
     5101 acttttctac aggtgttatt tgtcaaccag tttttctgag tcaacgtcat cgaaaatctt
     5161 atcaggtagc aaatatgtaa agattattat tacctaattt tttcatgttt aggtaggaat
     5221 gaaaagagtt acattagttg aaattgagtt atcaaggcga cacggataaa gtgtcagcat
     5281 aaaaaggaat tgataaatga gaaaactaag acatgacgaa atagtagcat ttcaaaaaat
     5341 tcagtctgac aaaaatagac gatttcctgt ttctgccgtt ttagagaaca ttcgaagttt
     5401 gcataatgta ggttccatgt tccgtacggc tgatggcgca ggattaaaca agctttatct
     5461 ttgcggtttt actggagctc cgcctcgtcc tgaaattgaa aaaagtgctt tgggaagcac
     5521 tgattatgta aactgggaac ggcaagagga gacgttaact ttggtacgac aacttaaatc
     5581 catgggtgaa cagattgttg tatttgaaca ttgtgaacag gcgaagagtt ataatcggat
     5641 cgagtaccga tacccctgtg tgcttgtctt tggtaacgaa gtgaatggag tatcgtctga
     5701 agtatgcgca ttggcggacg agactgtgct tattccaatg tatggcagca aacagtcgct
     5761 taatgtttca gtatcgtttg gtataggagt ttacaatgct attgcatctt tatcctaatt
     5821 tttataacaa tattattgct aacaagatag tatgttcttc agtgaattta gaatcttttt
     5881 gttcgttgat ggcaatatgg aaatttgcca tttttttcgt aatatcttct atgataatct
     5941 tcggcaggat aaaaatcttt ggcaggaagt atacttgtaa tcgcgtctgt tccattattt
     6001 cttaatgtat tgatcaatgt ttcggcaacg actttttgtt tgtaatctgt ataaaaaatt
     6061 acagacttgt attgctcgcc aatatctggc ccctgaccgt ctttttgtga agggtcatgt
     6121 atctcaaaaa aaagctctgc aagtttgctg tagctgattt ttgatgggtc gtatatgatt
     6181 tcgactgctt caacatgccc tgttttacca gaacaaactt cttcgtaggt tggtttatct
     6241 gttttgccac ctgtgtatcc tggcattgtc gaaacaatac cctcaatatt caagaaatgg
     6301 tattcaatgc cccaaaaaca gccacctgca aagattgctc tctcgtaatt cttgctggaa
     6361 tcagatattt tttctttagt tattgctaat gttttatcac ttatctcttt cagtggaata
     6421 aacttcatgg atattgaatt tacacaatgc cttacgtttt tgctggtaaa tccttccccc
     6481 ttaaagatat gtccaagatg cccaccgcaa ttagcacata ctatttcaga tcgcatgcca
     6541 tccttgtcag caatttcttg gactgcgcct tctacttgat cgtcaaagga cggccagcca
     6601 cagcctgatt taaatttggc cgttgattgg tataatggat tatcacattg tttgcaaaca
     6661 tatatccctg gttcatttag atctgtatat tcacctgtaa acggaggttc tgttcctttt
     6721 tcaattataa catacttttc agattcggtc agctttttca ttttgtacat cccattaaac
     6781 gtcatagccg ttctagaaat tgtaattttt cgaagttccc tattagtact tagcttggta
     6841 ttcttttgtg tgtaatatat ggattaagtt acaaaaagac aacggagtaa aggtgacaat
     6901 ttataatgat taaactggaa aggatttaat attataacca aaaacgttaa tgctgcataa
     6961 cacaacttta attataatat ttgcaatatg caatgaattg ggtgacaggt attttagtaa
     7021 aggaatgtaa tttgcaaatt aaaatattgc tgttccatcc tgttttatgt gaaattcggt
     7081 tttgcatcct gggcatatat agtcaccagg acgataaacc cttactaatg caccgcatgt
     7141 ggcacattca atcaccaagg gagaagggaa gtccaggatg ttttgactgt ctatattgat
     7201 tacaggttcc tcggaacgaa caagatcttc atcacggtac gatggagcgg gtgattgttt
     7261 gaaaagagat cccttgattg cttcaattgc ctgattcttg tcaggataaa ccgcaataaa
     7321 cttagcaatt tttagtattg aaaaaagaga tgttatttct ctgttgattc cgcataagac
     7381 tagagttccc ccttccttgt tcattttact actccataaa agcagaaaac tgattccgta
     7441 tgaggttaca aatgtcatct gtttgcaata tagtattact cttttcttgc cgtttgcaat
     7501 cagggaattg aggtttgcct ctagtgctac ggctgtttcc ccatttagtt tcccttctat
     7561 atcaattgtg attatatgtt cattggtaat cgcgctcatg atatcagttt cctctttttt
     7621 ttatgggtat tgcatcaaga attaccggtt cgactccgga ctgtaacggt cgtacgtcat
     7681 tgtaaaaatt tgagttattt acattaggag ccgggcttaa atccggttgc aaggctgatt
     7741 cacaaataat gtttgatgaa tttgtctgct tgcatcttaa tactgtataa agcaaaatag
     7801 caattaacga agcaataatc agagccatcg cgaaaattat atatttgata ttcgttaatc
     7861 gcgtaagctg tttattgata aagctgtata atgatttttt ggagtgcatt attgcaatga
     7921 caccttctcc attgtccgag
//
LOCUS       DAOTGZ010000130         7863 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_3064
            NODE_11103_length_7863_cov_5.364626, whole genome shotgun sequence.
ACCESSION   DAOTGZ010000130 DAOTGZ010000000
VERSION     DAOTGZ010000130.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868088
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 7863)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7863)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:22:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,785
            CDSs (total)                      :: 2,751
            Genes (coding)                    :: 2,732
            CDSs (with protein)               :: 2,732
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 12 of 19
            Pseudo Genes (internal stop)      :: 5 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7863
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11103_length_7863_cov_5.364626"
                     /isolate="AMR_MDS_3064"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..3022)
                     /locus_tag="P5123_09040"
     CDS             complement(<1..3022)
                     /locus_tag="P5123_09040"
                     /inference="COORDINATES: protein motif:HMM:TIGR00241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008047 - enzyme activator activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydratase activase"
                     /protein_id="HRX16447.1"
                     /translation="MNSQTALGIDIGSITSAIALIDSRKKIIKSSYYYHNGDITDSLK
                     NELKNYPLDSISIIAFTSDSPKIYSNCETYDSKVSLITAVKEFFPKMINVLHVGGEKF
                     SLIRFDEKGNYLDCRNNSSCAAGTGSFLDQQSKRLNLSSIQEFSDLAFSNRGSIPKIA
                     SRCAVFAKTDLIHAQQEGYSIGEICDGLSRGLAKNLCDSLFPENPDSPILFTGGVSQN
                     RAVIKHIEDITGQAVITSDMAHLAEAAGVAICAINDLDKKTDFAKSQYKTESIIIEEK
                     NEKKYHYSKLNTDIPDYPDFKGLESFLHKSERFIDTAAVETDLYMALNGLDLELYLGI
                     DIGSTSTKAVLVNENGDVVAGFYTRTAGKPVIAMQTIFEAIDNVASRYNASLKFKGAA
                     TTGSGRKFIGTIIDADLVLDEITAHARAAAQLNPDVDTIIEIGGQDAKFTTLKNGMVT
                     FSVMNNVCAAGTGSFIEEQAGKLGCPLSEYSIRAGNSKAPLSSDRCTVFMERDLNYYM
                     NAGYTNAEILTAVLHSVRDNYLSKVAVNRYIGDTIFFQGATAKNKALVAAFQYKLNKK
                     IIVSKYCHLTGALGSALQLADQKLKSSSFTGIEIYKKDIPIKNEICSICTNHCKIRVA
                     NINGRIAAFGFLCGRDYNTESYVSKNLSEYDLLKQRGIIQNFSRKKIEPDSPVIGIPA
                     ALHIFEELPLWQYFFNMLGLKTVTSINYKEGITAGKKIAGAEFCAPISNLHGHVEYLA
                     SRSDYVFLPTYLEEKQKDKNIRRHYCYYTQFVPVIIENLERYYKKKIVLSPMIKSLQG
                     SLGVKMQLFGMMKKIYPRASFLQVSSAYDKAVNFHNDAAGQYCENFASNCNYDDINVV
                     LLGRPYTTLVPSMNKGIIRIFENLGIKTFYQDMIPNQYNEDIEGLDQILRALHWNYGA
                     KIIESSAVVAKTNGLYPVLVTSFKCTPDSFVAEYYKNIFEAHNKPYLILQLDEHDSSV
                     GYETRIESAVRSFRNHYNANIKCPDSVKLSEELTN"
     gene            complement(3169..3783)
                     /locus_tag="P5123_09045"
     CDS             complement(3169..3783)
                     /locus_tag="P5123_09045"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRX16448.1"
                     /translation="MITKLAAEYTLLNAFDLSEDKSTVKIKITAQYNCKNDTDMQLTF
                     NPVSKDLCSVSINDTALTPADSNNQQCRYNFVLLPQLNHIEAVFETDILARSYKDGFY
                     SKYTPIYTVFTDFAQFRIPGTDNGFSATFKAMFSQKIPLFVKIFHGRPSIRFGSKAFD
                     EERGILHHGKVKNTNGICSSSVTIATALPDLMAVNFALGKRFIN"
     gene            complement(3816..5849)
                     /locus_tag="P5123_09050"
     CDS             complement(3816..5849)
                     /locus_tag="P5123_09050"
                     /inference="COORDINATES: protein motif:HMM:NF013736.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing phosphohydrolase"
                     /protein_id="HRX16449.1"
                     /translation="MKEKPDFNKIVFISHKASESIEKPATSVTHCMVGDIVKNITHAI
                     PADTDETPVAAIILIHESEYEYIRETVTIFKQWNFLYGFCLYIEDNHENNPVIQHDDI
                     HYIWTETLKPVEFRFAILNTFAALQHLFYKEKSSSDYLARLLDMKQDQENLIRIGRNL
                     SIERNSDKLLRLILKMSKTITGADAGSIYIIETNDKGKKQIRFKFSHTFSKELPLEEY
                     VLPFDKSSISGYVACTGIILNIPDVYKLNENDPIAFNNKFDIKNNYRSRSMLVIPMKT
                     HTGEIIGVIQLINSKKSEISRKSTGNEAFEIVLKTQEDFDNLVFPFAKRYEDLMESVA
                     GQAAIAIENSRLFNQIQNQFEEFVKASVNAIESRDPATSGHSFRVAEICKRMATYISD
                     CHNHPWAEIEYSPTKLKELEYAALLHDFGKVYVELAIFRKDKKLFPEEFENLSLKMDY
                     LYRTIELQFKIRQNEFLISGKNGSERAEQIHNLEEEMSQMLKRVKDIKSTLTKLNEPT
                     PLDEDPSELVSHIHNEISTLACCDISGKTMSILTDREIENLSIKRGSLNNEERREIEN
                     HVVHTYNFVKRIPWPKEFSNIPNIAWMHHEKLDGTGYPRGVTEQDIPLEAQMMAIADI
                     YDALTARDRPYKRALSHERALEIIESEAKLNKLNIKLFDVFKDANININNLGV"
     gene            complement(5962..6474)
                     /locus_tag="P5123_09055"
     CDS             complement(5962..6474)
                     /locus_tag="P5123_09055"
                     /inference="COORDINATES: protein motif:HMM:NF022072.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SRPBCC family protein"
                     /protein_id="HRX16450.1"
                     /translation="MKKLSLILLLFTKSILLAQNPISYSIDNNNILTAQFELDGSVDE
                     VWKLITDYENSDKIMPNVKKTVILSRKDNLITVETTVGSGPFSMTYTAIMTEIPKTKK
                     LLWQQKEGPFSKNRGSWHLTSLPGKRTKVTYEIEMDHKLLPDGLRQKLVKGSIPELYE
                     SLQKNLINKE"
     gene            6853..7455
                     /locus_tag="P5123_09060"
     CDS             6853..7455
                     /locus_tag="P5123_09060"
                     /inference="COORDINATES: protein motif:HMM:NF025338.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HRX16451.1"
                     /translation="MSTKETRKLIIDTAVGLFNERGVTTVTTNHIAAGCSLSPGNLYY
                     HYRNKEQIIREIFIQITEEYHSILDNDMNHIIRLFEMGTQILYRYRFFYRELATLITT
                     DPELKKLYRDNQLQKYSIIEKLILNMTKEGLLNEELVTKGLKALIESSWMHTDFWIPY
                     LSISGEDITPETISRGIRGYFELVLPFFTDRGREQIMGYF"
     gene            7582..>7863
                     /locus_tag="P5123_09065"
     CDS             7582..>7863
                     /locus_tag="P5123_09065"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRX16452.1"
                     /translation="MNYLKLLTPVLLFVCCSAVLTAASDNKSGANVSLTASTLPELQT
                     SADYTFFLPANTYVKIKGALSPVSVKADGEAVWTPLPFLQFVGSGSAGTG"
BASE COUNT         2287 a         1405 c         1512 g         2659 t
ORIGIN      
        1 tattagtcaa ttcttcgcta agtttcacac tatcaggaca ttttatattg gcattatagt
       61 gatttctgaa tgagcgtact gcagactcga ttcgagtttc atagcctaca ctagagtcgt
      121 gctcatctag ttgaagtatc aaatagggct tattatgtgc ttcaaagata ttcttgtaat
      181 actcggcaac aaaagagtcg ggtgtacatt taaacgatgt aacaagtact ggataaagcc
      241 cgttggtttt tgcaacaaca gcagagcttt caataatctt tgcgccataa ttccagtgaa
      301 gagcccgcaa aatctgatca agaccttcaa tgtcctcgtt gtactggtta ggtatcatgt
      361 cctgataaaa ggttttaatt cccaaatttt caaatattct tataatcccc ttattcatcg
      421 aaggtacaag agtagtataa gggcgtccta gtagcactac atttatatcg tcataattgc
      481 aattgctggc aaaattctca caatattgac cagcagcatc attatgaaaa ttgaccgcct
      541 tgtcataagc agatgaaacc tgtagaaaac ttgcgcgagg ataaattttc ttcatcatgc
      601 cgaatagttg catcttaaca ccgaggcttc cctgtagtga ttttatcatc ggagaaagaa
      661 caatcttctt tttatagtat cgttcaaggt tttcaattat aaccggtaca aactgtgtat
      721 aataacagta gtgacggcga atattcttgt ctttctgttt ctcttcaagg tatgtcggca
      781 aaaaaacata atcagaccta cttgcaagat actcaacatg gccgtgcaaa ttcgagatgg
      841 gtgcgcaaaa ttcggcacca gcaattttct ttccagcagt aataccctct ttgtagttga
      901 tactggtaac agtttttagt cccagcatat taaaaaagta ctgccaaagc ggaagttctt
      961 cgaatatgtg aagagcagca ggtataccaa taaccggaga gtcaggttca atcttttttc
     1021 gtgaaaagtt ttgaattatt cctcgttgtt ttaaaagatc atattcacta aggtttttag
     1081 aaacataact ttctgtattg tagtctcttc cacaaagaaa accgaaagcg gctattcgac
     1141 cattaatatt ggctactcta attttgcagt gattcgtgca tatcgagcat atctcatttt
     1201 ttattggtat atcttttttg taaatttcaa tacctgtaaa agaagaactt tttagttttt
     1261 gatcggccaa ctgcagtgca gaacctaatg ccccggttaa atggcaatat tttgatacaa
     1321 taattttttt attaagcttg tactgaaagg ctgctacaag agctttgttt tttgcggtag
     1381 caccctgaaa aaaaatagtg tctccaatat aacgatttac agcaactttt gaaagatagt
     1441 tatcgcgtac ggagtggagt actgctgtga gaatttccgc atttgtgtat ccggcattca
     1501 tataatagtt gagatcccgt tccataaaaa cagtgcatcg gtcgcttgat aggggagctt
     1561 ttgaatttcc ggctctgatg gagtattccg aaagtggaca ccccagtttg ccggcctgtt
     1621 cttcgataaa acttcctgtg cctgcagcac aaacattatt cattaccgaa aaggttacca
     1681 ttccgttttt taaagtcgta aactttgcat cttgaccacc aatttcaata atggtatcaa
     1741 catctggatt aagctgagcc gcagcgcgag cgtgtgctgt tatctcatca agtaccaagt
     1801 cagcatcgat tatggttccg ataaattttc ttccggaacc tgttgttgcg gctcctttaa
     1861 atttcaaaga ggcattatat cgagatgcta cattatcaat cgcctcaaat attgtctgca
     1921 tcgcgatgac gggtttacct gcagtacgcg tataaaatcc ggcgacaaca tctccgtttt
     1981 cattaaccag aacagctttg gtgcttgttg aaccaatatc aatcccaaga taaagttcaa
     2041 gatctagccc gtttagagcc atgtataaat cagtctcgac ggcggcagtg tcgataaatc
     2101 tttcagattt atgcaagaag ctttcaaggc ctttaaaatc ggggtagtcg ggaatatccg
     2161 tatttagttt gctatagtgg tattttttct catttttttc ttcaataata atggattccg
     2221 tcttgtattg actttttgca aaatcagttt ttttatcaag atcgttaatc gcgcaaatcg
     2281 caacaccggc ggcttcagcg agatgagcca tatctgatgt tattacagct tgacctgtta
     2341 tatcctcaat atgttttata accgctcgat tttgagaaac gcctccggta aaaagtatcg
     2401 gtgaatcggg gttttcggga aaaagtgaat cacacaggtt tttggctaat ccccgtgaga
     2461 gaccgtcaca gatttcacct atagaatacc cctcttgttg tgcgtgaata agatcggttt
     2521 tagcaaaaac agcgcatcga gaagcaattt tgggtatgga tcctctatta ctgaatgcaa
     2581 ggtcgctaaa ctcttgtatt gaagataaat ttaatcgttt ggactgctgg tcaagaaagc
     2641 tacctgtacc agcagcacag gatgagttgt tccggcaatc cagataatta cctttttcat
     2701 caaagcgtat caaagaaaat ttttcaccac cgacatgaag aacatttatc atttttggga
     2761 aaaattcttt aacggctgta atcagtgaaa ctttggagtc atatgtctcg cagttagaat
     2821 aaatttttgg ggaatctgat gtaaaagcta tgatggaaat cgagtccagc ggataattct
     2881 ttaattcatt tttgagagag tccgttatgt ctccgttatg ataataatat gatgatttaa
     2941 ttatcttttt tctactgtcg attaaggcta ttgcggaagt tattgagccg atatctatac
     3001 cgagtgctgt ttggctattc atctaagtcc tccagataaa ctgctttata tcaggagttt
     3061 ttttaaaaaa aattgcaacc aaaaaaactg gcttattttt aaaaatagac ttgtttatag
     3121 cacaaaatgt ataacgataa ggtttgcttg ttgtggttat gcataaattc aattaataaa
     3181 acgcttgcct agggcaaagt taacagccat caggtcggga agagcagtcg caattgtaac
     3241 cgatgacgag cagattccgt ttgtattttt tactttgccg tggtgaagaa ttcccctctc
     3301 ttcgtcaaag gctttacttc caaatctaat tgaaggacgt ccatgaaaaa ttttaacgaa
     3361 cagaggaatt ttctgtgaaa acatggcttt aaaagttgct gaaaaaccat tatcggtacc
     3421 aggtattcta aattgtgcga aatctgtaaa aacagtataa ataggcgtgt attttgaata
     3481 aaagccatcc ttgtaagacc ttgctaaaat atctgtttca aaaacagcct ctatatggtt
     3541 gagctgcggt agcaaaacaa aattataacg gcattgttga ttatttgaat cagctggggt
     3601 taaagcggtg tcattgatgc ttacgctgca aagatccttg gaaacaggat taaaggtcaa
     3661 ttgcatatcc gtgtcgtttt tacaattata ttgagctgtg atttttattt ttactgtaga
     3721 cttgtcttca cttaagtcaa aagcatttag cagagtatat tccgcggcaa gttttgttat
     3781 catgatttcc ccaaaataat aaaacagaag tttgattata ccccgagatt atttatattt
     3841 atatttgcgt ccttaaagac atcaaaaagt ttaatgttca acttattaag ttttgcttca
     3901 ctctcaatta tttccagagc tctttcatga gagagtgcgc gtttataggg acggtctctg
     3961 gcggtcaagg catcatagat atctgcaatg gccatcattt gcgcttcgag aggaatatct
     4021 tgctcagtta caccacgggg gtatccggtg ccatccagtt tttcatggtg catccacgcg
     4081 atatttggaa tatttgaaaa ttccttgggc catggtatcc gttttacaaa attatacgta
     4141 tgaacaacgt ggttttcaat ttcacgtctc tcttcgttgt tcaagcttcc ccgttttatg
     4201 ctaagattct cgatttcgcg atcggttaaa atgctcattg ttttgcctga tatgtcacaa
     4261 catgccagcg tgcttatctc attgtgaata tgtgatacca gctcagaggg atcctcatca
     4321 agcggggtgg gctcgttaag tttggttaat gttgacttta tatcctttac ccgctttagc
     4381 atttggctca tctcttcttc cagattatgt atctgttctg ctcgttcact gccgtttttt
     4441 cccgaaatta aaaattcgtt ttgtcttatt ttaaactgga gctctatggt acgataaaga
     4501 taatccatct ttaaactaag gttttcaaat tcttcaggga atagtttctt gtctttacga
     4561 aatattgcca gttcgacgta gactttgcca aaatcgtgaa gcaaagccgc gtattcaagt
     4621 tcctttagtt tagttggtga atattcaatt tcggcccagg ggtgattatg gcaatcagaa
     4681 atgtatgtag ccattctttt acatatttcg gctaccctga atgagtggcc tgaagttgct
     4741 gggtctcgag attctatcgc atttactgat gcctttacaa attcttcgaa ctggttttga
     4801 atctgattga aaagccggct gttttcgatt gcaatagcgg cttgaccagc aacagattcc
     4861 ataaggtctt catatctttt tgcaaaagga aacaccaagt tgtcaaaatc ctcttgtgtt
     4921 ttaagaacta tctcaaaagc ttcatttccc gttgattttc ttgatatttc gctttttttg
     4981 ctgttaatga gctgaataac tccgattatt tcaccggtat gagttttcat tggaattacc
     5041 agcattgagc gggaccggta gttatttttt atgtcaaact tgttattgaa agcgattgga
     5101 tcattttcat ttagcttata tacgtctgga atattaagta taataccggt gcatgcaaca
     5161 tatccggaaa tagagctttt atcgaaaggt agaacatact cctcaagggg caactctttc
     5221 gagaaagtat gtgagaactt gaacctgatc tgttttttcc ccttatcgtt agtttcaata
     5281 atataaatac tgccggcatc tgcccctgtt attgttttgc tcattttaag tataagacga
     5341 agcaatttat cgctatttcg ctcaattgaa aggttgcggc caatacgtat caggttttcc
     5401 tgatcctgct tcatatctaa aagacgtgcc agataatcgc tactgctttt ttctttataa
     5461 aatagatgct gtaaggcggc gaaagtattt aaaattgcaa aacggaattc cacaggttta
     5521 agtgtttctg tccagatata gtgtatatca tcgtgttgaa taacaggatt attttcgtgg
     5581 ttatcttcaa tgtaaagaca aaatccatac agaaaattcc attgcttaaa aattgtcaca
     5641 gtttcgcgta tgtattcata ctctgattcg tgtattagaa taattgctgc aactggagtc
     5701 tcatcagtgt cggcgggaat tgcgtgagtt atgtttttaa caatgtcacc aaccatgcag
     5761 tgagtaacag aagtagctgg cttctcgata gattcggagg ctttatgtga tataaatact
     5821 atcttgttaa aatcgggttt ttctttcata aattcctgtt gttaatatgt tgaataatga
     5881 aaacgtgtta tcaatcagtc aacataaaaa agcaggattt gatgttaaaa aagcctgttt
     5941 ggattatttt gttttatgaa cttattcttt gtttatgaga tttttctgca gtgactcata
     6001 aagttcggga atacttcctt ttacaagttt ttgtcgtaat ccatcaggta agagtttatg
     6061 gtccatctcg atttcgtaag ttaccttcgt acgtttgcca ggcagtgatg ttaagtgcca
     6121 actacctctg tttttgctaa atggaccctc tttttgttgc caaagtagtt tttttgtttt
     6181 tggaatttcg gtcataattg cggtgtatgt catcgaaaac gggccactgc ccacggtggt
     6241 ttcaactgtg atcaaattgt ccttccggga gagtattacg gtttttttta cgtttggcat
     6301 tattttatct gaattttcgt aatccgttat caacttccaa acttcatcca cactgccgtc
     6361 aagctcaaac tgtgctgtta aaatattgtt gttatcgatc gaatagctta tggggttttg
     6421 tgctagtaaa atagacttgg taaaaagaag taatattaga gaaagttttt tcattataca
     6481 acctttgatg tcattatctt gaaaaacagt gtgataaatg tttataccct ttgttacaat
     6541 gctgtataac atatacgaga aataaacact ttataaattc acatttgcat tcatgataga
     6601 atgttatatt ttttaagagc cagtgatgtt attgacttta tttcagaact tcttcacata
     6661 taaagtgata cggttattgt ggaagaataa ttaaagcgta tgattaatca aaagtcaaat
     6721 tattcgatat ttagttattg taaagaataa aaaggaaaaa gtctttttga tatccgttag
     6781 gtttttaatt caatttaagg gagattgaaa acttgatatt tatctaacaa caaatttttt
     6841 tggggtgcga ttatgtcaac gaaagaaaca agaaagctta taattgatac ggccgtaggt
     6901 ctttttaatg agcgtggagt aacgaccgta actacaaatc atattgctgc aggctgttcc
     6961 cttagtcctg gaaatctcta ttatcattat agaaacaagg agcagattat acgtgagata
     7021 tttattcaaa ttactgagga gtatcattcc attctggata atgatatgaa tcatattatt
     7081 agactgttcg agatgggaac acaaatactg taccgttata gattctttta tcgagaattg
     7141 gcaacattga ttaccacaga cccggaatta aaaaaacttt accgggataa tcaacttcaa
     7201 aagtattcta taatcgagaa gctgatatta aatatgacaa aagagggttt gctcaatgaa
     7261 gaacttgtta caaaaggtct aaaggctttg atagaatctt cctggatgca tacagacttc
     7321 tggataccat atttatctat aagtggagaa gatattactc ccgaaaccat ttcacgaggt
     7381 attcgtggat attttgaact tgtactgcct ttttttaccg ataggggcag ggagcagata
     7441 atgggatatt tttagaaaaa ttatctctga atattgatta acataaattt ttccaggata
     7501 actcactttt ttttgtaact gcttgacaca tttctgatct tgattgttgt attagagtaa
     7561 atactctaaa aggtggtaag catgaattat ctaaaattat tgactcctgt tttattattt
     7621 gtttgctgtt ctgctgtgtt aactgctgca tcggataaca aaagcggcgc taacgtatct
     7681 ttgactgcct caactttgcc ggaacttcag acaagcgcag actacacgtt ttttttgccc
     7741 gcgaatacgt atgtaaagat caaaggagcc ctatcgcctg tatcggttaa agcggacggt
     7801 gaggccgtat ggacgccgtt gccgtttttg cagtttgttg gttccggatc ggctggaaca
     7861 ggg
//
LOCUS       DAOTGZ010000131         7853 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_3064
            NODE_11119_length_7853_cov_4.957553, whole genome shotgun sequence.
ACCESSION   DAOTGZ010000131 DAOTGZ010000000
VERSION     DAOTGZ010000131.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868088
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 7853)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7853)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:22:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,785
            CDSs (total)                      :: 2,751
            Genes (coding)                    :: 2,732
            CDSs (with protein)               :: 2,732
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 12 of 19
            Pseudo Genes (internal stop)      :: 5 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7853
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11119_length_7853_cov_4.957553"
                     /isolate="AMR_MDS_3064"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..775)
                     /locus_tag="P5123_09070"
     CDS             complement(<1..775)
                     /locus_tag="P5123_09070"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024917.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HRX16453.1"
                     /translation="MKTNNTHIIKKCSNDTDRRKLIDFNSLIHGEDCGRLTEKLLKKH
                     PQIHDENFIFIQTSKNEIISSVALIPWQVDFHNIKLNVFELGIVGTDPKERGKGLSRE
                     LTNFFIDMIEDLNYDISIIQGVPGFYHNFGFHYAIPLDIHLNLPLYLVDEPFDNSNYK
                     IRKASNNDAIFIAKLFNQHVKSYDLHPIISADIVEYCMDCTTDSEYDAEYYIIKTNED
                     DAGYFKINLHGFGAGLIVSEISDMSYSACMQIIAYCSKLA"
     gene            1274..2143
                     /locus_tag="P5123_09075"
     CDS             1274..2143
                     /locus_tag="P5123_09075"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRX16454.1"
                     /translation="MKNVVVLFTVLFFNILLFSEENTENINDIKIWKSDSKKIVPILA
                     EIRKYKINDKDTEIQIEYKKNKQKEISKSLNKSYVGKTLSLKRAHIVDITEVKEKNEK
                     HMRISMSVLNNYYTKTRKKIATDNDIEIIVKKIYKNKILKGYKLVDIDDINKKNTPFL
                     TKKEIYILKELYGLSHDSTVDLFFLLVKINTETESCEDCYDFTGAYNIQMDIPFPNGY
                     GSYNYGLYLNSNESSFDKYNTLDIVINYVTKDKDYAMNLSKDDIIYLNCKIVSIEYTN
                     SNAYHEKVTLYVK"
     gene            complement(2395..3330)
                     /locus_tag="P5123_09080"
     CDS             complement(2395..3330)
                     /locus_tag="P5123_09080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014322235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0098662 - inorganic cation transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="calcium/sodium antiporter"
                     /protein_id="HRX16455.1"
                     /translation="MLLIQQFALLAVSIVLLWFGANYLVDSAEKIANRFGVSQLVIGL
                     TIVAFGTSAPEFAVTVNAALRGQASISVGNIVGSNIFNLGFILGLVALFHPVAANRKL
                     VYRDGIFMVGSAVILLVFFLTGGYTLSRIEAAVLFILLVFYLGFLFTKKDPIDTDEIS
                     NEKAKWYDFLIAPLSVIVIASGGQLLVTSSVSIAKFFGISEWVIAITIVAAGTSAPEL
                     VTSLAAVLKGKHDISAGNLVGSNIFNILGVLGVAGMINPLTIDASSFESLILLVGMIV
                     LSILFFRTGWKVSRKEGIILILCGLAMWAVDFVTM"
     gene            complement(3347..3676)
                     /locus_tag="P5123_09085"
     CDS             complement(3347..3676)
                     /locus_tag="P5123_09085"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRX16456.1"
                     /translation="MIKLKSGKIIEVEQDFDQFFQTIMTEIINESVTTAQNAGDKVKE
                     QDAFNKVLLKEIMDNSIFVTHQIFELAKVNKNLSKFLVTGFLFNSIVLSVPDLSSQFL
                     DTNKTIH"
     gene            complement(3663..5093)
                     /locus_tag="P5123_09090"
     CDS             complement(3663..5093)
                     /locus_tag="P5123_09090"
                     /inference="COORDINATES: protein motif:HMM:NF012784.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S1 RNA-binding domain-containing protein"
                     /protein_id="HRX16457.1"
                     /translation="MDNEITENFEELLNQSFSKLDNNYEPGDQVTGLIVNEDDELVFV
                     DIGYKHEAIALRSEFSEDDLSIYTKKAANFIISQTRGTEIELTKKIGAGFVSDDLILY
                     AIENQIPVYGRIVSSSDAGYDILIGELKAFCPGSQLGKSELSLYQANKNSLLPFAVIE
                     KKRGNYLVSSRVLHEKEQKIVIEKLSKELKPGQSDTGTVTRTEEYGVFVKLDCGLEGL
                     VPRRELDHSRFITPESFNVGDTVKAVITSLDWTTNKHTLSIKASIEDPWTTHASLISE
                     GVNLTARVCGFIKDGVFAEIYPGVDGFIHKSLLSKQKRINDPKDIFTIGQQIEVSVIS
                     VDIAKKRISLDYMSGESDPWDSIEYTSNNRDTATIESSMPAGLVIRLSNGLEAFVPKG
                     ELLGRSEEPRYIVGSKIDIVLIETDKNRRRAVASEKQLGEVEQKEQLKDFIDGQKQAE
                     HGSSLGALLGDKFKELQKKVDANDKT"
     gene            5425..5877
                     /locus_tag="P5123_09095"
     CDS             5425..5877
                     /locus_tag="P5123_09095"
                     /inference="COORDINATES: protein motif:HMM:NF025030.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HEAT repeat domain-containing protein"
                     /protein_id="HRX16458.1"
                     /translation="MKKALLLIIAVTVSFGTLFSQDSEMSASDYVRMLSSKNPAKIIE
                     ACNWFGENENKEGFSKMVELVSHSDESVRIWSSANLGLLKDVEAVNPLIDQLEKESSA
                     DVRYTIILSITRLGLKEDAQKDRLKALRRKEKDPIVLDYIAKMEEKLK"
     gene            complement(5959..7416)
                     /locus_tag="P5123_09100"
     CDS             complement(5959..7416)
                     /locus_tag="P5123_09100"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRX16459.1"
                     /translation="MPQNIYSSKNDYIQQKIKNADDIKYKLQKYFYEMSDLSYENGSR
                     KGDYQIRDDVDQITEQINKFYEYLRLFETVKQGNMHALEDILVFFQNNFTNTCTVTDA
                     MEKVLSSLEYQTSLDIQHNKQLDDHRLERKKNIFVSELKQKIKLFEVKFSTVIAPALK
                     EFLNDINSLYFFYSVENNIQKPKSVSSDLSLTHSTPIESELYTYIWIYIKINTKTHTP
                     TEITDLIASEINKMGMYNQLLNMSQMTSNQLREHIKNIIIDIPNNITTNQNPLYVNYE
                     PNRRNEELQTHIDAVVHLKAIINSLYNLNTGSPSKFLNDFSDAKDLLKAKHNQRFMFH
                     STSHSEITLKTICDYHSNSLMFIVSWILTESKNKNVFRTHMGPLIKIMNEIESFIALT
                     GTALLLITEKTNVQLPVLTGDMKMYIPAALAKLITETVISNCEHLDHAFSEISVDCSL
                     KGLACDPVFKKKTDIMWESHRDTVRRIMKYLKRYL"
     gene            complement(7455..>7853)
                     /locus_tag="P5123_09105"
     CDS             complement(7455..>7853)
                     /locus_tag="P5123_09105"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRX16460.1"
                     /translation="FTTNVTQIIKNSVSLALPINFNRGNEVRLPFVNVILKGDLKLKN
                     EFDEPETVEVTVFKLAEHEVVVRSSGNHIDDNRCEIFFTLENFAITAPARIIAEKTVS
                     ENDVSYYTLKFMELSKPAEKVIRNYIYEHI"
BASE COUNT         2479 a         1307 c         1384 g         2683 t
ORIGIN      
        1 tggccaattt gctgcaataa gcaataattt gcatgcaggc actataactc atatcagata
       61 tttcactgac aattagtccg gccccgaaac cgtgaagatt aattttaaag tatcctgcat
      121 catcttcatt agtttttatg atgtaatact ccgcgtcgta ttctgaatct gtggtgcaat
      181 ccatacagta ttcaacaata tctgcactaa tgatcgggtg caaatcataa ctcttaacat
      241 gctgattaaa tagctttgca ataaaaatcg catcattatt actggccttt ctaatcttgt
      301 aattgctgtt atcaaaaggt tcatctacaa gatatagcgg taaattcaga tgtatatcga
      361 gaggtatggc ataatgaaat ccaaaattat gataaaagcc cggtacgccc tgtattattg
      421 aaatatcata gttaagatct tctatcatat ctataaaaaa atttgtaagt tcacggctta
      481 gcccttttcc tctctcttta ggatctgttc ctactattcc caattcaaaa acattgagct
      541 tgatgttgtg gaagtcgacc tgccacggga taagagccac agatgagata atttcattct
      601 tgctggtttg tataaatata aaattttcat catgaatttg tgggtgtttt ttgagcaact
      661 tttctgttag tcgtccacag tcttcaccgt gaatcaggct gttaaaatct attaactttc
      721 ttcggtctgt atcattagag cattttttta ttatatgagt gttatttgtt ttcatcttga
      781 tctccattag ttttatattg gtaaaaatta tacatgtgcc ctgtgctgtt tgacaatcga
      841 ttatatctct ggacataatt atacaaaaca gttattagtg ttgtatgaat tatcataagt
      901 aatttgttct gtttcaataa tgaatgatta ttatcatgac aattttgggc acaaaaagag
      961 aggccgcagt tgatacggtt tttttcatgt aaataaaaag gccttatcga ctgcggttta
     1021 aaccagcata aaactccgaa aatatatcag gaatattact gacaaatagt tattgataca
     1081 gtattttctt cacttgaaga taagaaaata caattcataa tgaaaacagc tgatcttgta
     1141 aactggaatt ttaattcaat gagaaaaaaa cgcgtaccaa gctctgttac ggtactatta
     1201 ttgactaatg atattaaatt ttgtattttt gttattgtag ataaaacgtg ttttgtctca
     1261 tgtggaggat gttatgaaaa atgtagttgt tctatttaca gttttgtttt ttaatatatt
     1321 actcttttcc gaggaaaata cagagaatat aaatgacata aaaatatgga aaagtgatag
     1381 taaaaaaata gttcctattt tagctgaaat aagaaaatat aaaattaatg ataaagatac
     1441 tgaaatacag attgaatata aaaaaaataa acaaaaagaa ataagtaaat ctttaaataa
     1501 aagctatgtt ggaaaaacat tgtctttaaa aagagcacat attgttgata ttacagaagt
     1561 taaagaaaaa aatgaaaaac atatgcgaat ttctatgtcg gttttaaata actattatac
     1621 taaaaccaga aaaaaaattg cgactgataa cgatattgaa attatagtaa aaaaaatata
     1681 caagaacaag attttaaaag gttataaatt agtcgatatt gatgacataa acaaaaaaaa
     1741 tactccattt ttgaccaaaa aagaaatata tattttaaaa gaattgtatg gtttaagcca
     1801 tgattcaaca gtagacttat tttttcttct tgttaaaatt aatacagaaa ctgagtcatg
     1861 tgaagattgt tatgatttta ctggagccta taatattcaa atggatattc cgtttcctaa
     1921 tggttatggt tcctataatt atggtctata tcttaattcg aacgaatctt ctttcgacaa
     1981 atataatact ctagatatag ttattaatta tgtaacaaag gataaagact atgccatgaa
     2041 tttatccaaa gatgatatta tttatcttaa ttgcaaaatt gtcagtattg agtataccaa
     2101 tagtaatgcg tatcatgaaa aagtgacact ttatgtgaaa taacattgct gttgtatgac
     2161 ttcttttcac aataaagtta gccgttaatt attttgttgc gatacggaat taccatttgt
     2221 tggcggttaa agtaatagat gttaaacatc ttttgtgagc aacagatatc aatttattag
     2281 acgaaatatt tttagcaagc tatagtaatt ttggttttta gatatatttt aagaagtaac
     2341 atattaatat ggattaatat gtcacattct ttataaaaac acacttttga tcagctacat
     2401 tgttacaaaa tctacagccc acatagccaa accacaaagt atcaggatta ttccctcttt
     2461 tcgagatacc ttccagccag ttctgaaaaa taaaattgat aatacaatca ttcccacaag
     2521 caaaatcagc gattcaaacg aagaggcgtc aattgtgagg gggtttatca ttccggcaac
     2581 acctaaaact ccaagaatat taaatatatt gctacctaca aggtttcctg ccgaaatatc
     2641 atgtttgccc ttaagaactg ctgcaagaga ggttacaagc tcgggagccg atgttccagc
     2701 tgcaacgatt gttattgcga ttacccactc agatatgcca aaaaacttgg cgatggatac
     2761 agacgatgtt acaagcaact gtccgcccga tgcaataacg ataacagata ggggagctat
     2821 taaaaaatcg taccatttcg ctttttcgtt agatatctcg tcggtatcta ttggatcctt
     2881 tttggtaaaa agaaaaccaa gataaaaaac aagaagaata aaaaggacag cggcttcgat
     2941 tcttgatagg gtgtaaccac ctgttaagaa aaatacaaga agtattacag ccgatccgac
     3001 cataaatata ccgtcacggt ataccagttt acggttggca gctaccgggt gaaaaagtgc
     3061 cacaagaccg agtataaaac caagattgaa aatatttgat ccgacaatat ttccgacaga
     3121 aatgcttgct tgcccgcgaa gtgctgcatt tacggttaca gcaaattcgg gtgcggaggt
     3181 tccgaatgcg acgatggtaa gaccaataac aagctgagag accccgaagc ggtttgctat
     3241 tttttcggcc gagtctacaa gatagttagc tccaaaccat agcagtacta ttgacacggc
     3301 tagcagggcg aactgttgta ttaataacat tattttttac ctgtttttaa tgtattgttt
     3361 tgtttgtatc aagaaactga ctggataaat ctggtaccga tagaacaatg ctgttaaaca
     3421 gaaaaccagt aacaagaaat tttgaaagat ttttgttaac ctttgcaagt tcaaaaatct
     3481 gatgggtaac aaaaatgctg ttatccatta tctcttttag aagaaccttg ttaaatgcat
     3541 cctgctcttt gaccttgtct ccggcatttt gagctgttgt tacagactca ttgattattt
     3601 cagtcatgat ggtctgaaag aattgatcaa aatcttgttc aacttcaatt atttttcccg
     3661 atttaagttt tatcatttgc atccactttt ttttgaagtt ctttaaattt atcccccaaa
     3721 agtgctccaa gggaagagcc gtgctctgcc tgtttttggc catcaataaa gtcttttagt
     3781 tgttcttttt gttctacttc gcctagctgt ttttcactgg ccaccgcacg acgtctattc
     3841 ttgtcggttt caataagaac aatatcaatt tttgagccaa caatgtaacg tggttcttca
     3901 ctgcgaccta aaagttctcc tttaggtaca aaggcttcca gcccgtttga caatcttata
     3961 acaagcccgg ctggcatact actttcaatt gtagcagtat cccggttatt actcgtatat
     4021 tcaatagagt cccatggatc actctctccc gacatatagt ccagggaaat cctttttttg
     4081 gctatatcaa ctgaaatcac gctaacttcg atctgctgtc ctattgtaaa aatatctttt
     4141 ggatcgttaa tacgcttctg tttcgagagt agcgatttgt gaataaaacc gtcaacaccg
     4201 ggataaattt ctgcgaaaac gccatctttt atgaatccgc atactcttgc ggtcaaattc
     4261 actccttcac tgataagtga tgcgtgtgtg gtccatgggt cttctatgga tgcttttatg
     4321 cttagggtat gtttattggt tgtccaatcc agagatgtaa taacggcttt taccgtatct
     4381 ccaacattga aagattctgg ggtaatgaat ctggaatggt caagttcacg acggggaaca
     4441 agtccttcaa gaccgcaatc aagttttaca aatactccgt actcttcagt tcttgttact
     4501 gttccggtat cactctgtcc aggttttagc tctttagaga gcttttcgat aacaattttt
     4561 tgttcttttt catgcagaac gcgtgaagat acaagatagt tgcctctctt tttttcaatt
     4621 accgcaaagg gcaacaggga atttttattt gcttgataga gtgatagttc tgatttacca
     4681 agttgtgaac caggacaaaa tgctttaagt tcaccaataa gaatatcgta acctgcatcg
     4741 gaggatgaaa cgatacgacc ataaacgggt atctgatttt caatagcgta caaaatcagg
     4801 tcatcggata cgaatcctgc accaattttt ttcgtaagct ctatttccgt gcccctggtc
     4861 tggctgataa tgaaattcgc agcttttttt gtataaatcg agagatcatc ttcagaaaat
     4921 tctgaacgaa gagctattgc ttcgtgtttg tagccaatat ctacaaaaac aagttcatcg
     4981 tcttcattaa caataagccc agttacctga tctcccggct cgtagttatt gtccagtttg
     5041 ctgaaagact ggtttagtag ttcttcaaaa ttttcagtga tttcattatc catgatagtc
     5101 tccgaatagt gctttgacaa taaatcgtcg atatgccaaa actaacattg ttaatctttt
     5161 tatcaaacag aatattttcc gattgccaaa atttaaccgt atcgtatcag attctatcgg
     5221 ttaaagccca aatgggctta ttaaccataa tattctataa aaaatgttgt ttttgatgtt
     5281 aagtctaaat aaattgttga cagtcgccgt tcaatatatt tattattgaa tgaaaggtcg
     5341 ttgtctttta aaaaggcagc ctgttaattt tatgatgcat atatctgttt tgcgttaatt
     5401 ctataaaaac tttgaggtat taaaatgaaa aaggcacttc tattaattat cgcggttact
     5461 gtatcgttcg gtactctctt ttcacaggat tccgaaatgt ctgcgtcgga ttatgtacgg
     5521 atgttgtcca gcaaaaatcc tgccaagata atcgaggcat gtaactggtt tggcgagaat
     5581 gaaaacaaag aaggtttctc gaaaatggta gaactcgtat ctcatagcga tgaatcagtt
     5641 agaatatggt cctctgctaa tcttggtctt ttgaaagatg tagaagccgt taatcctcta
     5701 attgatcaac tagaaaaaga gagttcggct gatgtacgtt acactataat attgtcaata
     5761 acccgtttgg gacttaaaga agatgctcaa aaagatcgtc ttaaagctct aagacgtaag
     5821 gaaaaggatc ctattgttct cgactatatt gcaaaaatgg aagagaagtt aaaatagaat
     5881 tgtaaacaaa aaagttaaat aacaaataac tgtcttgatt aaacctccct attagggagg
     5941 tttttttatg atgaagtttc aaagatatct ttttagatat ttcatgattc ttcttactgt
     6001 gtctctatgg gattcccaca tgatatccgt ttttttcttg aagaccggat cgcaagcaag
     6061 cccctttaat gagcaatcaa cacttatttc agaaaacgcg tgatcaaggt gttcacaatt
     6121 actgataact gtttcagtaa tcaatttcgc tagagctgcc gggatgtaca tcttcatatc
     6181 gcctgttaac acaggtaatt gaacatttgt tttttccgtt attagtaaaa gagctgtacc
     6241 tgtaagagca ataaatgact ctatctcatt catgatcttg attaagggcc ccatatgtgt
     6301 tctaaataca tttttgtttt ttgattcggt taatatccag ctgacaataa acataagaga
     6361 atttgaatgg tagtcacaga ttgtttttag agtaatctcg gaatgagatg tcgaatgaaa
     6421 catgaatctc tggttatgtt ttgcctttaa aagatccttt gcgtcactga aatcgtttaa
     6481 aaacttggat ggagatccgg tattaagatt atagagacta ttaattatag ctttaagatg
     6541 cacaactgca tcaatatgag tttgtagttc ctcattgcgt ctattaggct cgtagttaac
     6601 atatagtgga ttttgattgg ttgttatgtt gttaggtata tcaataatga tattttttat
     6661 gtgttcacgt aactggtttg aggtcatttg ggacatattc aataattgat tatacatacc
     6721 catcttgtta atttcggagg caataaggtc agttatttct gttggagtat gtgtttttgt
     6781 atttatctta atataaatcc agatgtatgt ataaagctcg ctctctatag gagtgctgtg
     6841 tgtcaggctt aagtcggatg atacggattt gggcttttgt atgttgtttt ccaccgagta
     6901 aaaaaaatag agtgagttaa tatcattaag aaattctttt agagcaggag caataacagt
     6961 actaaatttc acttcaaaga gcttgatctt ctgtttgagt tccgatacaa aaatattttt
     7021 ctttctttct aaacgatggt catcaagttg tttgttgtgt tgaatgtcaa ggctggtctg
     7081 atattcaagc gatgatagaa ccttttccat agcatctgta acggtacatg tgttagtaaa
     7141 attgttctga aaaaagacta aaatatcttc cagtgcatgc atattgccct gtttaactgt
     7201 ttcaaacagc cgtaagtatt cgtaaaactt attaatctgt tcggttattt ggtcaacatc
     7261 gtcacgtatt tggtagtcac cttttcgtga tccgttttcg tacgataagt cactcatttc
     7321 gtagaaatat ttttgcagtt tgtatttaat atcgtcggca ttctttattt tttgctgaat
     7381 gtagtcattc ttggaactat atatattttg aggcataaga actccaaatt gtttttttaa
     7441 actttttgct gttatcaaat atgttcgtaa atatagtttc gtataacctt ctctgcgggt
     7501 ttacttaatt ccataaattt aagggtataa tatgatacat cgttttccga aacagtcttt
     7561 tcggcaatga ttcgtgctgg tgctgttatt gcgaaatttt ctagcgtgaa aaatatttca
     7621 catcggttgt catcaatatg attgcctgaa ctgcgtacaa caacttcgtg ttctgccagt
     7681 ttgaatacag tcacttcaac agtttcgggc tcatcaaatt cgttttttag tttcagatct
     7741 ccctttaaaa tcacgttaac aaatggtaat ctgacttcat tgcctcggtt aaaatttatg
     7801 ggcagagcta gggagacgct gtttttgata atttgagtta catttgttgt aaa
//
LOCUS       DAOTGZ010000132         7793 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_3064
            NODE_11236_length_7793_cov_5.475446, whole genome shotgun sequence.
ACCESSION   DAOTGZ010000132 DAOTGZ010000000
VERSION     DAOTGZ010000132.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868088
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 7793)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7793)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:22:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,785
            CDSs (total)                      :: 2,751
            Genes (coding)                    :: 2,732
            CDSs (with protein)               :: 2,732
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 12 of 19
            Pseudo Genes (internal stop)      :: 5 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7793
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11236_length_7793_cov_5.475446"
                     /isolate="AMR_MDS_3064"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(163..375)
                     /locus_tag="P5123_09110"
     CDS             complement(163..375)
                     /locus_tag="P5123_09110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011937537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system HicB family
                     antitoxin"
                     /protein_id="HRX16461.1"
                     /translation="MRRYLVVFEQTETGYSAYVPDLPGCIATGDSKKEVEELIYEAIS
                     FHMEGLKEDNLAIPEGRAEAEVLLFG"
     gene            complement(535..1119)
                     /locus_tag="P5123_09115"
     CDS             complement(535..1119)
                     /locus_tag="P5123_09115"
                     /inference="COORDINATES: protein motif:HMM:NF014068.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DJ-1/PfpI family protein"
                     /protein_id="HRX16462.1"
                     /translation="MNKKVLLFLPRGFELYEASVFTDVFGWSRLSGVTGVDLDTASFE
                     REVQCNWNLTVKSEFLYEDIDPDSYDALAIPGGFKRSGYYNDAYDKRLLELIAGFNRS
                     NKYIAAVCTASLCLAKAGVLNGRSAVTYDLLNGYFQSQLAGSGALVEHQPFFVDNNII
                     TCCGPGAAADCAFKLLSCLTDEDNMNKIKDMMRF"
     gene            complement(1184..1855)
                     /locus_tag="P5123_09120"
     CDS             complement(1184..1855)
                     /locus_tag="P5123_09120"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRX16463.1"
                     /translation="MFKKNLICFVVIFLTFSVFADDVDSFTSVKKRIEDEVLPDDGLI
                     EIEDNPLTVNEALALMIPHYYKVQYAGLIGFMSIGVGYTVKNIYEPVFMLGYLDPYFG
                     NSDVTVTSFAWKNNFVLFGSISPDYLKFYAGTSVLWCYTNGTFGKLPPHYPDDYYFQN
                     RVHLAPHMGLEYKKIFSSGFAKAGGIFMEIGTFDNYIKSGFTNDYVKFYDIWNLAFGL
                     TVYIK"
     gene            complement(1886..2752)
                     /locus_tag="P5123_09125"
     CDS             complement(1886..2752)
                     /locus_tag="P5123_09125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016777527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HRX16464.1"
                     /translation="MKLDGYASRFIKNTNICLVGKLFRILHYKLGILILIISFVGCDL
                     IEYSPFQVDDSVAGKNRNGKAINKIITKSSDTFKPFKIAIIADSHTYYDDLCDQVNTI
                     NKLKDIDFVIHMGDFTDNGIYKEFVWFTEIIDELKLPILVMIGNHEYLGNGEVMYEKV
                     FGERNFTVIYNDCRFVFWDDVVWERNGKDPDFEWLQENIEEGEDYSYQFVFAHIQPWD
                     RQFSAGNAFFYDYLLKTNGVDLSVHGHAHVFLYEEYYEGIPYLIIDSSPAREITVLEI
                     NQDSFEVENIAL"
     gene            complement(2865..3707)
                     /gene="nfo"
                     /locus_tag="P5123_09130"
     CDS             complement(2865..3707)
                     /gene="nfo"
                     /locus_tag="P5123_09130"
                     /EC_number="3.1.21.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004511109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribonuclease IV"
                     /protein_id="HRX16465.1"
                     /translation="MKYIGAHVSAAGGVQNAPLNAQKIGANAFAFFTKNQRQWDAPPL
                     DENSVTLFKENLVKAGIEPQFVLPHDSYLINIGNPDDIKRKRSLDALTDEAERTELLG
                     LQLLNFHPGAHLGLINEEGCMDLIADGINHVLDSTDRTIMVIENTAGQGTGVGHRFEH
                     LAYIISRVKDKNRVGVCIDTCHLFAAGYDIRTQETYSKTFADFDSIVGFEYLRGMHLN
                     DAKSEYQSRVDRHNSLGAGKIGIDAFKMIMRDSRFDNMPLILETIDDSLWPKEIKMLR
                     GFLG"
     gene            complement(3728..6433)
                     /gene="polA"
                     /locus_tag="P5123_09135"
     CDS             complement(3728..6433)
                     /gene="polA"
                     /locus_tag="P5123_09135"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: protein motif:HMM:NF004397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006261 - DNA-templated DNA replication
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase I"
                     /protein_id="HRX16466.1"
                     /translation="MKTLYVIDGHALCYRAYYAFINNPLTNSSGQNTSAIYGFARMVL
                     QLIREQNPDYLAVAFDPPGKTFRFDMFADYKANRQKMPDDLRSQIEEIKNMVTVFDFP
                     VLIQEGFEADDILGSVAQQYSDECQVYLVTGDKDAYQLVNDSVKIYANSKGVSEFIIY
                     DEEAVKEKLGVTPEQVVDYMALVGDSADNVPGVKGIGPKGAAALIQKYGTIENIYENI
                     DELKGKQKQNLLDYKDDAFLSKQLVTIRHDLKLNFALNEAEFKGFDDARCSEYFNKLE
                     MRSIAGDYFGGTPVVYEKFDKSLVKYTCVTTRAQLDAALKTIVQADIIAVDTETTDLH
                     HQKARLVGISVSFKEKQGYYFPVYGGGLFNDCDTEFDSEELLKLIRDPLENEKIKKVG
                     QNIKFDIMILRKAGINLKGVYFDTMIASYLLNPAERHNMDDMAERLLNYNTIKYSEVV
                     GKGKNSRPLAEVPLDEISVYAAEDTDITLRLYNALKPEIEKHEKLKSLFYDIEMPLVE
                     VLADMEYRGVLIDSAHFKKLSLQNDEQLKKTEESIYGHAGAVFNINSTKELASLLFDK
                     ERLGLKAVKKTKTGFSTDISVLEALRKEHPIIEELIRYRTISKLKSTYIDTLPALVEP
                     STGRIHTSYNQTVVTTGRLSSSDPNLQNIPIKDDFGRLIRKGFVAPQGYSIVAADYSQ
                     IELRLAAHISGDPAMCRAFNENADIHTLTASSIFGVSKDKVTADMRRQAKVVNFATIY
                     GVSPYGLSKQIDLSVSEAKHFIDRYFETYPGFKDYMEKVITFARENGFVETTCGRRRY
                     VPEINSKSQFPREGAERVAVNTPIQGTSADMIKIAMISLEKHIHEYSLKSRLVMQVHD
                     ELVFELADEEEFFIDVIKKEMESAIELSVPVLVEVGRASNWEEAH"
     gene            complement(6606..>7793)
                     /locus_tag="P5123_09140"
     CDS             complement(6606..>7793)
                     /locus_tag="P5123_09140"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HRX16467.1"
                     /translation="ANKKLAIERERADLATKREDIFMAKMSHELRTPLNGIMGITDLI
                     IDSQECAHKSSYLDMIREAAETLKYLIDDLLDLSKIENDLIVLNNNTFVLKNFLSGIV
                     AEFFVSAQKKDIRLESNISDELDCRVSTDGHRLRQIYSNLLTNAIKYTNNGGVIHFDA
                     SPLKSPGGDYLLCVRVSDNGVGIPECDHKKIFSSFYRSIESYNNQSSGIGLGLYIVDR
                     LVDLFKGTITVESREGEGSTFTVTIPVAKACEQVDNAADIETVHPKCKPLDILVAEDN
                     RINAVFITSFLEKEGHSITLVDNGNDAVAKACANFYDLVLMDIQMPLLSGTDAVAKIR
                     EYEAKNMRAPLVIAAITAYASENEQIEIRKSGFDYYLSKPLDSAKLRSILRNIPQKNG
                     NAD"
BASE COUNT         2350 a         1758 c         1323 g         2362 t
ORIGIN      
        1 cttaatgaga aagaagggta atgaacaatg aaaagtgaaa agtaaataat ggttattgct
       61 caacaaataa gtactacagc gaattaatcc ctacttgtca gtttactata taaaagtaaa
      121 aagatagcac gatataaata tcggttaaaa acctgttaat agtcaaccaa agagaagaac
      181 ttcagcctca gccctacctt ccggtatagc aagattatct tcttttagac cttccatgtg
      241 aaagctgata gcctcataga tcaactcttc gacctctttt ttactgtcac cagtagcaat
      301 gcaaccgggc aaatccggaa catacgcgct ataaccggtt tctgtttgtt caaaaacaac
      361 aagatatcgt ctcactattt ttctcctttt tccagacctg actgtttcag tatagagttt
      421 aacgtcccag gtgcaacatc aacttcacaa acctgttaat agtcaacagt acaagactaa
      481 aagctttgac agaaccaaca cctaacaaat tagattatat tttaattatg tctttcaaaa
      541 ccgcatcatg tcttttatct tattcatatt atcttcatct gtcaaacagg acaaaagttt
      601 aaaagcgcaa tctgccgctg ctcctggacc acagcaggtt ataatattat tatctacaaa
      661 aaaaggctga tgctcgacga gggctcctga gccagcgagc tgagattgaa agtaaccatt
      721 caaaagatca taggtaacag cactgcgccc atttaaaaca ccagcttttg ccaaacataa
      781 tgaagctgta caaacagcag cgatatattt atttgagcgg ttaaaaccag caatcagttc
      841 aagtaatctt ttatcataag catcattata gtagcccgat ctcttgaatc ctccaggtat
      901 tgcaagagca tcataactgt caggatcaat atcttcgtat agaaattcac tcttaacggt
      961 taggttccaa ttgcattgca cttctcgttc aaacgaagca gtatcaagat ctactcctgt
     1021 aacccccgat aaccggctcc atccaaaaac atctgtaaaa actgaagcct cataaagctc
     1081 gaaacctcgc ggcaaaaaaa gcagtacctt cttattcata gtcaagttat ccacagccgg
     1141 aaattaaata attaagaatt tacctcgcat actccattct tcgtcatttt atataaactg
     1201 ttaaaccaaa agctaaattc catatgtcat aaaattttac ataatcattt gtaaacccgg
     1261 acttaatgta attatcaaaa gttcctatct ccataaatat ccctccggct tttgcaaaac
     1321 cgctagaaaa aatttttttg tattcaagac ccatatgtgg agccaaatga accctgtttt
     1381 gaaaataata atcatcggga taatgcggag gcagtttacc aaatgttcca ttcgtataac
     1441 accaaagaac cgaagttcct gcataaaact tcaaataatc aggacttatt gaaccaaaca
     1501 aaacaaaatt attcttccat gcaaaagatg ttaccgttac atcactgtta ccaaaatatg
     1561 gatcaagata gccaagcata aagactggtt cataaatatt ctttactgta taacctacac
     1621 ctattgacat gaaacctatt aatcctgcat attggacttt ataatagtga ggtatcatca
     1681 acgccagagc ctcattaaca gttaacggat tatcttcgat ttcaatcagg ccatcatccg
     1741 gtaatacttc atcttcaatt ctttttttta cagaagtgaa agaatcaaca tcatcagcaa
     1801 acacagaaaa cgttaaaaaa ataacaacga aacagattaa atttttctta aacataattc
     1861 atcctcaata aacaccatga atcaactata atgcaatatt ctcgacttca aaggaatcct
     1921 ggtttatttc taaaactgtt atctctcttg caggacttga atcaattatt agatagggaa
     1981 taccctcgta atattcctca tacaaaaaca cgtgtgcatg gccatgaaca gataaatcga
     2041 caccatttgt tttaagaaga taatcataaa aaaaggcatt acctgcagaa aattgtctgt
     2101 cccatggttg tatatgagca aaaacaaatt gataactata gtcttcgcct tcctcaatat
     2161 tctcctgcaa ccactcaaaa tcaggatctt ttccattgcg ttcccataca acatcatccc
     2221 aaaatacaaa acggcaatca ttataaatta cagtaaaatt tcgttccccg aaaacttttt
     2281 catacataac ttcgccattt ccaagatact cgtggttgcc aatcataact agaatcggta
     2341 atttcagttc atcaattatt tctgtaaacc agacaaattc cttatatatt ccattatcag
     2401 taaaatcccc catatggata acaaaatcta tatcctttaa tttgttaatt gtattcacct
     2461 ggtcacatag gtcatcgtaa taagtatggc tatcggcaat tatcgcgatt ttgaagggct
     2521 taaaagtgtc agaactcttt gttatgatct tatttattgc tttgccattt ctgttttttc
     2581 cggcaaccga atcatcaact tgaaatggac tgtactcaat taaatcacag cctacaaaag
     2641 aaattataag aattaaaata ccaagtttat aatgcagtat tcgaaataat tttccaacca
     2701 aacagatatt tgtgttttta ataaatcgac ttgcataacc atctaatttc attattttct
     2761 cctataaaca ttactgcaat atatccggtt tttttgcttt accatatatc cacaatacct
     2821 taacaaaata ttacaggtaa atgtccaatt aattttcagg aattttaacc taaaaacccc
     2881 cgaagcatct tgatctcttt tggccacaga gaatcatcta tcgtctcaag gataaggggc
     2941 atattatcaa aacggctgtc cctcattatc attttgaaag catcgatacc tattttacca
     3001 gcgccaagac tattatggcg gtcaacacgg ctttgatatt cacttttagc gtcattcaga
     3061 tgcattccac gcaaatactc aaaccctaca atactatcaa agtccgcaaa tgttttgcta
     3121 tatgtctctt gtgtgcgaat atcataaccc gcggcaaaca gatgacaggt atcaatgcag
     3181 actccaacac gattcttgtc cttaacacgt gatattatgt aggcaagatg ctcaaacctg
     3241 tgaccgactc ccgttccctg gccggctgta ttttcaatta ccattatagt tctgtctgta
     3301 gaatccagaa catggtttat tccgtctgct ataagatcca tgcaaccctc ttcgttaatc
     3361 agccccagat gcgcccccgg gtgaaaatta agcagctgca atccaagaag ttctgtccgc
     3421 tcggcctcat ctgtcaacgc gtcaagagag cgttttcgtt ttatatcatc agggtttcca
     3481 atatttatca gataactgtc atgaggaagc acaaactggg gttcaatacc ggcctttaca
     3541 agattttctt taaaaagagt tacactgttt tcatctaacg gcggcgcatc ccactgccgt
     3601 tggttctttg taaaaaaagc aaaggcattt gctccaatct tttgagcatt aagaggcgcg
     3661 ttttgcactc cacctgccgc cgaaacatgt gctccaatat atttcatagg gattccttta
     3721 ttatctatca atgagcctct tcccagttgg aggcccggcc aacttcaact aacacaggaa
     3781 ctgataattc aatcgcggat tccatctctt tctttattac atcaataaaa aattcttctt
     3841 catctgcaag ttcaaatacc agctcgtcat gcacctgcat cacaagacgg ctcttaagac
     3901 tgtattcatg tatatgtttt tccagcgata tcatggctat ctttatcata tccgctgaag
     3961 ttccctgtat gggagtgtta acagctacac gctctgctcc ctcacgcgga aactggcttt
     4021 ttgaatttat ctctggcaca taacggcgtc ttccacatgt cgtttcgaca aatccgttct
     4081 cgcgcgcaaa agttataacc ttttccatat aatccttaaa acctggatac gtttcaaaat
     4141 agcggtcgat aaaatgtttg gcctcactta ccgaaagatc tatctgcttt gatagcccgt
     4201 aagggctaac gccgtaaatg gttgcaaagt ttaccacctt agcctgtcgt cgcatatcag
     4261 ctgttacctt gtctttactg acaccaaaaa tactggaagc tgtcagcgta tgaatatcgg
     4321 cgttttcgtt aaaagcgcgg cacattgccg ggtcacccga tatatgcgct gccagtctta
     4381 gctctatctg tgaataatca gccgctacga tgctataacc ttgcggtgcg acaaagccct
     4441 tacgtataag acggccaaaa tcatctttga ttggaatgtt ttgcaaattg ggatcacttg
     4501 aacttagacg tcctgttgtt acaacagtct ggttatacga ggtgtgtata cgcccagtcg
     4561 atggttctac aagagcagga agagtgtcga tataggtact tttaagttta cttatagttc
     4621 gataacggat aagctcttca attatcggat gctcttttcg caaggcctct aagaccgata
     4681 tatcggttga aaaaccggtc tttgtctttt ttacagcctt tagaccaagt ctctctttat
     4741 caaacagcag tgatgccaat tcttttgtcg agttaatgtt aaaaacggca ccggcatggc
     4801 catatatgct ctcttcggtt tttttaagct gctcatcatt ttgaagagac aactttttaa
     4861 aatgcgcgct atcaatcagc acgccccggt attccatatc ggccagcact tctacaagcg
     4921 gcatctctat atcgtaaaaa agacttttca gtttttcgtg tttttcgatt tcaggtttaa
     4981 gagcgttata aagtcttaac gtaatatcgg tatcttccgc agcatagacc gatatctcat
     5041 caaggggaac ctccgcaaga gggcggctgt tcttaccttt gcccactact tcagaatatt
     5101 tgattgtatt ataatttaac agccgctcgg ccatatcatc catattatga cgctctgccg
     5161 gattcaaaag ataagatgct atcatggtgt caaaatagac acctttaagg tttatacccg
     5221 cttttcgcag tatcataata tcaaacttta tgttttgccc gaccttttta attttttcgt
     5281 tttcaagcgg atctctgatc agtttcaaaa gttcttccga atcaaactcg gtatcacaat
     5341 cgttaaaaag cccccctcca taaacaggga aataatagcc ctgcttctct ttaaatgaaa
     5401 ccgagattcc aacaaggcga gctttctgat gatgaagatc ggttgtttcg gtgtcgacag
     5461 caataatatc cgcttgaaca atagttttta gcgccgcatc gagctgggcg cgagtagtca
     5521 cacaggtata tttgacaaga gacttgtcaa atttttcata tactacggga gttccaccaa
     5581 aataatcccc agcgatagaa cgcatctcga gcttattaaa gtattcgcta caacgtgcgt
     5641 catcgaatcc tttaaattca gcctcgttaa gcgcgaaatt taattttaga tcatggcgta
     5701 ttgtaacaag ctgctttgat aagaaggcat catccttata atctaaaaga ttctgtttct
     5761 gtttgccttt taactcgtct atgttttcgt atatattttc aatagtgcca tatttctgta
     5821 ttagcgcggc tgcccctttt ggccctattc ccttgacacc cggaacatta tccgcgctat
     5881 caccaaccag cgccatatag tctacaacct gctcaggagt caccccaagt ttttccttta
     5941 cagcttcttc gtcataaata ataaactctg agactccctt gctgttggca tatattttta
     6001 ccgaatcgtt aaccagctga taggcatcct tgtcaccagt aaccagataa acctgacact
     6061 cgtccgaata ctgctgtgca acagagccca gtatatcgtc ggcctcgaaa ccctcctgta
     6121 tcagtaccgg aaagtcaaaa accgtaacca tattctttat ctcttctatc tggctgcgaa
     6181 gatcgtcggg cattttttgc cggtttgcct tatagtcggc aaacatatca aaacggaaag
     6241 ttttacccgg cggatcaaag gcaacagcaa gatagtcggg gttttgctca cgtataagct
     6301 gaagaaccat acgggcaaac ccgtaaatgg cggaagtatt ctgtccgctg ctgtttgtga
     6361 ggggattatt tataaaggcg taataggctc tgtagcagag agcatggccg tcaatcacat
     6421 ataatgtttt cacaggggat tcatcctttt gtttaacatg ttttacagag ctcaacactc
     6481 gtaatcacat tattttacaa aatcccctct gagcgtcaac ccttattgaa agattcgcta
     6541 actgtcatct gaagcaagct ataatgatat tttgaaacag ggtagttttt cgtagttccc
     6601 tagtattaat cagcattccc atttttttgc ggaatattgc gcaaaataga gcgaagcttc
     6661 gcggaatcaa gaggctttga aagataataa tcaaaccccg atttgcgaat ctctatctgt
     6721 tcgttttccg aagcgtacgc cgtaatggcc gcaataacaa gcggcgccct catattttta
     6781 gcctcgtact ctcgtatttt tgcaacggca tctgttcccg aaagaagagg catctgaata
     6841 tccataagaa caagatcgta aaaatttgca caggccttag caacggcgtc gttgccgtta
     6901 tcaaccaagg ttatgctgtg accctctttt tcaagaaaag atgttataaa aaccgcgtta
     6961 atacgattgt cttcggcaac aagtatatca agaggtttac atttcgggtg aactgtttct
     7021 atatcggcag cattatctac ctgttcacaa gcctttgcaa ctggaatggt cactgtaaac
     7081 gtacttccct ctccctcgcg gctttctacc gtgattgtac ccttaaaaag atcaacaagt
     7141 cgatctacta tatataagcc aaggccaata ccggacgact ggttattgta cgactcgatt
     7201 gagcggtaaa acgaactaaa tattttttta tggtcacact ctggtatgcc aactccgtta
     7261 tcgcttaccc ttacgcagag cagataatca ccacctggcg acttcagggg gcttgcatcg
     7321 aaatggatta caccaccatt attggtatac ttgatcgcgt ttgtaagcaa atttgaataa
     7381 atctgtctaa gacggtggcc atctgtgctt acacggcagt caagctcatc cgatatattc
     7441 gattcaagtc tgatatcctt tttttgtgcg ctaacaaaaa actcggcgac aatgccgctc
     7501 agaaagtttt ttaatacaaa cgtattgtta ttcaaaacga tcaggtcgtt ttcaattttc
     7561 gataaatcaa gcaggtcatc tatcagatat tttaaagtct cggctgcctc tctgatcatg
     7621 tcaagatagc tgctcttgtg agcacactcc tgactatcga ttataaggtc ggtgatgccc
     7681 attattccgt taagtggagt acgcagctcg tggctcattt tggccataaa gatatcttcg
     7741 cgtttagtag ccagatcggc tctctctcgc tcgatagcaa gttttttgtt ggc
//
LOCUS       DAOTGZ010000133         7718 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_3064
            NODE_11366_length_7718_cov_4.885945, whole genome shotgun sequence.
ACCESSION   DAOTGZ010000133 DAOTGZ010000000
VERSION     DAOTGZ010000133.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868088
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 7718)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7718)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:22:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,785
            CDSs (total)                      :: 2,751
            Genes (coding)                    :: 2,732
            CDSs (with protein)               :: 2,732
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 12 of 19
            Pseudo Genes (internal stop)      :: 5 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7718
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11366_length_7718_cov_4.885945"
                     /isolate="AMR_MDS_3064"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..296
                     /locus_tag="P5123_09145"
     CDS             <1..296
                     /locus_tag="P5123_09145"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRX16468.1"
                     /translation="IVSYFLDRLGDNQPDTAGKRKSNFSIKTKIANTKIQKKLIISLV
                     FIISLFLSANIYLKVSLGLSGKIAEDLYQSVLISKYVLETQNRMNTIQGIYDD"
     gene            432..1823
                     /locus_tag="P5123_09150"
     CDS             432..1823
                     /locus_tag="P5123_09150"
                     /inference="COORDINATES: protein motif:HMM:NF012245.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein"
                     /protein_id="HRX16469.1"
                     /translation="MIFEAENFVKDYTNLFDNAKSNIALEDAQESIYSVKRIQNHIDN
                     YNAKLDMLKTVTEKNSAQLHELSLKKNELAQKASTSLLLFSLFSGIVISIIISKSILK
                     SISLFQNIFSKGSSGDLNSEYPVYANSKNEFNNLGLMFNSFISKTNIVIKQVKKVSEE
                     LSFSSSDLTDTTSAFSENTQTQAATSEQITATLEEVTAGINSISENTKKQHNKLIEVI
                     KLMDNLSEEISITANSIADTRNKSIDIAKQARRGNDALKIMSKSISEIASSSSEVTEI
                     IQIIGNISSEINLLSLNAAIEAARAGESGKGFAVVADEISKLADQTASSISQIAALIE
                     KNNDEISKGMQNINDTISGISKIIDSVESIDNMMNTIISDVKKQQDANKYVNISIKDL
                     SKLTDEVKFSIEEQRTAANEIMNSMNSINNMIQSDAASAEEIAGNTESLSDMAERLKT
                     DIQFFKLKDNSSL"
     gene            2217..4403
                     /locus_tag="P5123_09155"
     CDS             2217..4403
                     /locus_tag="P5123_09155"
                     /inference="COORDINATES: protein motif:HMM:NF015155.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MMPL family transporter"
                     /protein_id="HRX16470.1"
                     /translation="MKKYYFLIIMALLITIFFSFSLSNNSTESEVYAKNEGELLIPQS
                     NAAIAIVDIKQAGLSLERSADLPILKELHNDFAKLKNVQKVESLLNAFRVISDKDEII
                     VNRVLPDNINSVTDEYLASTMSTINEFPELKSYISKNQDTLIYYIYFSNSTKPRLIYD
                     NLINMQKDWESRLHFDFTGRSPILAATESFLTKDIGTAFPVLIIMIFCIFLLFKNLKV
                     IFISLFTILLSLGAAYGFSRFLGLHDSPLLLLIPVFGLGLLSDYLIHYFYHRLYSPDI
                     ADKKDAKRQLLFPLSLTAISTITGFLSLTLINGSGHVQLGIIIAFSIIIIWFGVFYWI
                     NYQSYPSKNAKLFPGFQKIQTIFFLILLKHRYICYFLILLSLFWGAFQLKNLVVEPYP
                     IEQLPENTTIQIADKRINSEFYGTLPFYLEIDTGEKNGILKKSTLINLQQIHNIMDSS
                     SAGYSFSLLTVLKRMHYYFMGSEETLLSSNEFDDQYDSLIEQYLLYFSSSVDPLEYES
                     LLNNSYRLFSIKGLIYYRDYSDLEKFIGTINEFKKIIPQNWEVNFYGMASELQKEHDN
                     LRNNWIISFLTSGFLIFLTVFIFYRKVDLALISLLPGIISMIISFGIINIAGISIDAF
                     SIIFVAIITGLVVDYSIHTLAAINRIKKITSIEDSFKQIVGYSGLPVFLSFVTSLLSF
                     MVLFISSFRGAQNLGLILITSLGLAFFLSLYLIPLIILPIRFKKES"
     gene            4404..5183
                     /locus_tag="P5123_09160"
     CDS             4404..5183
                     /locus_tag="P5123_09160"
                     /inference="COORDINATES: protein motif:HMM:NF028441.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_process: GO:0042953 - lipoprotein transport [Evidence
                     IEA];
                     GO_process: GO:0044874 - lipoprotein localization to outer
                     membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane lipoprotein-sorting protein"
                     /protein_id="HRX16471.1"
                     /translation="MKKIIITLISFVLIISPLSAGKTVEEILAEFDKTISVPSLQGTF
                     TVKLISKNGDTREIKARAYQKLVDNKQNNRLFIFDFPPRVRGTGLLVHSFLDGRDNNM
                     WIYLPTVKRVKRIALESSGGGYFMGSDFTYRDLIANDLNNMKFELLEEKTMNGTESYV
                     IKCWGKNQEVIKDLGYSYMISYYKKENSVMHRREFFDSDSKLLKIYQVDDFHVSGKYH
                     YPTKITMTNVQTEHKSIIEVTEISTKDIPERYFTTRYLTDK"
     gene            5227..6519
                     /locus_tag="P5123_09165"
     CDS             5227..6519
                     /locus_tag="P5123_09165"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRX16472.1"
                     /translation="MQSRFIAYILITLSILFLVPDCFAVEHGFKFVSFADYYGGIEYS
                     DGYENLRSRLFFRPEYSANFEDFGSEIIISANLWAQPIGDPNFIDPYDIPNEVYFSKK
                     TELFDFTVGQKLITYGFADIYGPFNAPHSTNQTLLSLDDQFDGRRPDPLFQLRFYPTL
                     EDIVEFTYIPLTRPDKEQPDDVYLPETHDTVVWDDSSYLTDNLHSFFMNYKRYGENLD
                     IQLLYGWYTEHTPDFVVTNISSSNSYDIKPEYHHKHTIGAAYATRVGNTTLSQDFALN
                     ITDDFSGKDLAAQDSDITVNTQILANLPWNILSQYTIVYCYFFNHDEYDRQSDSKAAE
                     YLAEQFHDFHNQPYQHILFFVGHFEKSFLREKLKAQLNMGFFFSPNIYVAPRLAYSIT
                     DTSRLETGIEYTFGKPKDIDLRRNPSNDNFFIRFIMQY"
     gene            6542..6907
                     /locus_tag="P5123_09170"
     CDS             6542..6907
                     /locus_tag="P5123_09170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012505408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA thioesterase"
                     /protein_id="HRX16473.1"
                     /translation="METFSMVRPEHLNHHGFLFGGQLLLWVDEYAWLAAARDLPGCRL
                     VTRAMDSISFIRSAPVGSILRFDISQTYKGQTSAKYQVSVFCDPQTGAHEYNIFSTNV
                     IFVNVDQNCNKKPFCNDKN"
     gene            6907..7218
                     /locus_tag="P5123_09175"
     CDS             6907..7218
                     /locus_tag="P5123_09175"
                     /inference="COORDINATES: protein motif:HMM:NF026248.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4491 family protein"
                     /protein_id="HRX16474.1"
                     /translation="MISLNLKGICIGLYTFFLIGILHPIVIKGEYYLGKRICILFFFC
                     GLLFLIFSVLIIDLFFSCCLGITGFMLLWSIKETFDQEKRVIAGRFPKNPKRKYPGKI
                     L"
     gene            7215..>7718
                     /locus_tag="P5123_09180"
     CDS             7215..>7718
                     /locus_tag="P5123_09180"
                     /EC_number="2.3.1.286"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002981981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070403 - NAD+ binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Sir2 family NAD-dependent protein deacetylase"
                     /protein_id="HRX16475.1"
                     /translation="MKRLTVLTGAGISAESGLKTFRDSNGLWEEYDVMQVASIEGWNK
                     DPELVLQFYNDRRKQLEFVYPNRAHLGLMELEKYFEVSIITQNVDNLHEKAGSSRVLH
                     LHGELTKACSVENKNLVKDIGYREILPGETGEDGKQLRPYIVWFGEAVPAIGKAIPLV
                     KEADYLAV"
BASE COUNT         2591 a         1392 c         1299 g         2436 t
ORIGIN      
        1 tgattgtctc ctatttttta gacagattag gcgacaacca gcctgacaca gcgggcaaac
       61 ggaaatcaaa cttcagtata aaaacaaaaa ttgcaaatac caaaatacaa aaaaaactta
      121 taataagtct tgtttttata atttcactgt ttttatccgc taatatatat ttaaaagtat
      181 cgctcgggtt atctggtaag attgcggagg atctgtacca gtcagtatta atatccaagt
      241 atgttcttga aacacaaaac agaatgaata ctattcaagg catttatgat gactgaattg
      301 actatttaaa caataacaat tttactaatg caaatttagt attatccaaa caaacatcat
      361 ctgaattaga taatttcagc aaaaatatta ttgttattaa agaacaactt aatagcgaat
      421 caagtagcaa attgatcttt gaagctgaaa atttcgttaa agattataca aatctttttg
      481 ataatgcaaa atccaatata gctttagaag acgctcaaga aagtatttat tctgttaaaa
      541 gaatacaaaa ccatattgat aactacaacg caaaattgga catgcttaaa acagtaacag
      601 aaaaaaattc ggctcaactt cacgaactta gtttaaaaaa aaatgaattg gctcaaaaag
      661 catctacttc tcttttgcta ttttcacttt tttcaggaat agttatttcg atcattatct
      721 ctaagagcat tttaaaatcc atatctttgt ttcagaatat tttcagtaaa ggaagttcag
      781 gggacttaaa ctctgaatat cctgtttatg caaattcaaa aaatgaattt aacaatcttg
      841 gcctgatgtt taacagtttt atatctaaaa cgaatatagt tataaaacag gtcaaaaaag
      901 tatccgaaga gttgagcttc tcatcaagtg atcttacaga tacaacttct gcattttcag
      961 aaaacaccca aacccaggca gcaacctccg aacagattac cgcaaccctg gaagaggtaa
     1021 cagccggaat taacagcata tccgagaaca caaaaaaaca gcataataaa ctcattgaag
     1081 taataaagct catggataat ctttctgagg aaataagtat tacggctaac agtattgctg
     1141 atacaagaaa caaatcaatt gacattgcca agcaagcacg gagaggaaat gacgctctta
     1201 aaataatgtc gaaaagcatc agtgaaattg cttcaagttc cagtgaagta actgagataa
     1261 tccagatcat tggaaacata tcaagtgaaa taaaccttct ctcgctaaac gccgcaatcg
     1321 aggcagcaag ggccggtgaa tcgggaaaag gctttgctgt tgttgcggat gaaatatcaa
     1381 aactggctga tcaaacagca tcaagcataa gccaaattgc tgctttgatt gaaaagaata
     1441 atgatgaaat ttcaaaggga atgcaaaata tcaatgatac aatttcgggc attagtaaaa
     1501 ttattgatag tgttgaatca attgataata tgatgaacac cataatatcg gacgtaaaaa
     1561 aacagcaaga cgcaaataaa tatgtaaaca tcagcataaa ggatttgagc aaactaacag
     1621 atgaagtcaa attttccata gaagaacaga gaacagctgc aaatgagata atgaactcca
     1681 tgaacagtat aaacaatatg atacaatcag acgcggcaag cgcggaagag atcgcgggta
     1741 acacagaaag cctttctgac atggcggaaa ggcttaaaac agatattcag tttttcaaat
     1801 tgaaagacaa ttcttcttta taattttcca gacagatcga cagatcaata aaatactatt
     1861 taacaagaac ttcacttatt taactaattt gctaaatcat tatttatacg cgatatgttt
     1921 tttctttaaa cccgtgcaac cggttacacg gattaatatt atctatccat aatgaatttc
     1981 aaaaaaattt tggacaaatt tttatagtct attgttttaa aatagtatgt ttacttacgg
     2041 cttaataaaa aatctcacaa ttcagagcaa tactgctcaa aaaacttcaa attcattttt
     2101 tgcaagctta tgtataaaaa tatgttgact gtcttgaaag actcacaatt aaataataga
     2161 taacaggttc tataatttat cgtttttccg cattacttgc ggatggggtt taatttatga
     2221 agaagtacta ttttttgata ataatggctt tgcttataac gattttcttt tctttttctt
     2281 taagtaataa tagcacagag agtgaggttt acgctaaaaa tgagggtgag ctgcttattc
     2341 cacaaagcaa tgcggcaatt gctattgttg acatcaagca ggctggactt tcacttgagc
     2401 gctctgcgga tcttcccata ttaaaagaac ttcataatga tttcgctaaa ctaaaaaacg
     2461 ttcaaaaagt cgaatctttg ttaaacgcct ttcgagtaat atctgacaaa gatgaaatta
     2521 ttgtaaaccg ggttcttccc gataatataa actctgtaac cgatgaatat cttgcttcaa
     2581 ccatgtccac aataaacgaa tttccggaat taaaatcata tatcagcaaa aatcaggaca
     2641 ctttgatata ctatatatat ttctctaaca gtacaaaacc caggcttatt tacgataatc
     2701 ttataaacat gcaaaaagac tgggaaagca gacttcattt tgattttacg ggtcgttccc
     2761 caattcttgc tgcaactgaa tcatttttaa ccaaagatat cggcacagct tttccagttc
     2821 ttattattat gatattttgt atttttctgt tatttaaaaa tttgaaggtt atatttattt
     2881 cactgtttac cattctttta tcccttggag cagcctacgg cttttcccgt ttcttgggac
     2941 ttcatgactc tccactactt ttattaatac ctgtatttgg tttgggactg cttagcgact
     3001 accttattca ctatttttat cacagactct attctccgga tatcgccgat aaaaaagatg
     3061 caaaaagaca actacttttc cccttatccc ttacggcaat atctacaata acgggatttc
     3121 tatcattaac tcttataaac ggctccggac atgtgcaact tggtataata atcgcgtttt
     3181 ctattatcat tatttggttt ggagtttttt actggatcaa ttatcaatct tatccttcaa
     3241 aaaacgcaaa actgttccca ggttttcaaa aaattcaaac catatttttt cttatactat
     3301 taaagcatcg ctatatctgc tattttttaa tcttgctaag ccttttctgg ggtgcgtttc
     3361 aacttaaaaa cctggtagtt gaaccatatc ctattgaaca gctacctgag aatacgacaa
     3421 ttcaaatcgc cgataaaaga ataaattccg aattttacgg gacactgccc ttttatctgg
     3481 aaattgatac tggtgaaaaa aacggcattt taaagaaaag cacattaata aatttacaac
     3541 aaatacacaa tattatggac agttcaagcg ccgggtattc attctccctg ctcactgttt
     3601 taaaaagaat gcactactac ttcatgggtt cggaagaaac gttgctttca agtaatgaat
     3661 ttgatgatca gtacgattct ttaatagaac agtatctgct ctatttttct tccagcgttg
     3721 atcctctgga atatgaatct cttctaaata attcttacag actcttctca ataaaaggtc
     3781 taatctatta ccgcgattat agcgatcttg aaaaattcat cggaacaatt aacgaattta
     3841 aaaaaatcat accacaaaat tgggaagtta acttctacgg catggcatct gaactgcaaa
     3901 aagaacatga caaccttaga aacaattgga taatctcttt tttaacatca ggttttctaa
     3961 tttttttaac ggtttttatt ttttatcgta aagttgatct tgcgctcata agccttctgc
     4021 caggcataat aagcatgata atttcatttg gcataataaa tattgccggt atatctattg
     4081 atgctttttc tataattttt gtggccataa taactggcct tgttgttgac tatagtatac
     4141 acacacttgc tgccataaac agaattaaga agataaccag tatagaagat agcttcaagc
     4201 aaatagttgg ctatagcggc ttacctgttt ttttaagctt tgttacttca cttctttcgt
     4261 ttatggttct ttttatatct tctttccgcg gagcgcaaaa cctgggattg attctaataa
     4321 cttccttagg tcttgctttc tttttaagcc tgtatttgat accgcttata atattaccaa
     4381 tacgtttcaa aaaggagtca tgaatgaaaa aaataattat tactttaatt tcttttgtac
     4441 ttattatctc tcccttgtct gccggtaaaa ctgtagaaga gattcttgcg gaatttgaca
     4501 aaacaatctc tgtaccttct cttcagggca ctttcaccgt taaacttatt tcaaaaaacg
     4561 gtgacaccag agaaattaag gcgagagcct accagaagct tgttgataac aaacagaaca
     4621 acaggttatt catctttgat tttccgccaa gagttcgagg aaccggactt cttgtgcact
     4681 cttttcttga cggaagagac aacaacatgt ggatatatct tccaacagtt aagcgtgtca
     4741 agcgcattgc tctggagagc tctggtggtg gatattttat gggaagtgat tttacataca
     4801 gagaccttat tgctaatgac ttgaataata tgaagtttga acttcttgaa gaaaagacga
     4861 tgaacggcac tgaaagttat gtaataaaat gctggggcaa aaatcaggaa gtaattaaag
     4921 atttaggcta ttcttacatg attagttatt ataaaaaaga aaacagtgtt atgcacagac
     4981 gtgagttttt tgattctgat tcaaaattgc ttaaaatata tcaggttgat gattttcacg
     5041 tatctgggaa ataccactac cctacaaaaa ttactatgac aaacgttcag accgaacata
     5101 aatcaattat tgaggtaact gaaatcagca ctaaagatat tcctgaaaga tattttacaa
     5161 cacgttatct gactgataaa taactaactg ttcattacaa cacagcaaaa ttaccgagga
     5221 tatcaaatgc agtcaagatt tatagcttat atattaatta ctctatcaat actcttttta
     5281 gtacctgatt gttttgcagt ggagcatggc tttaaattcg tctctttcgc ggattattat
     5341 ggtggtatag aatactccga tggttatgag aaccttcgga gtcgcctttt ttttagaccc
     5401 gaatattcag ctaattttga agatttcggt tctgaaataa ttatttcagc aaatctttgg
     5461 gcacagccta tcggggaccc caacttcata gatccatacg atataccaaa cgaagtatat
     5521 ttttcaaaaa aaaccgaact tttcgacttc actgttggac aaaaactgat tacatacggt
     5581 tttgccgata tttatggtcc tttcaatgcc cctcacagta caaaccagac ccttctcagc
     5641 cttgatgacc agttcgatgg aagacgtcct gaccctttat ttcagctgcg cttttatcca
     5701 acacttgaag atattgttga atttacatat ataccgctta ccagacccga caaagaacag
     5761 ccggatgatg tatatttacc tgaaacccac gatactgtag tatgggatga cagctcttat
     5821 ctaacagaca accttcactc tttttttatg aactacaaaa gatacggcga aaaccttgac
     5881 attcaactgc tttacggatg gtacacagaa cacacgcccg attttgttgt tacaaacata
     5941 agcagctcta attcatatga tattaagcca gaataccacc ataagcacac tattggcgcg
     6001 gcctatgcca caagagtcgg gaacacaact ctaagtcagg atttcgccct gaatattacc
     6061 gatgattttt ccggtaagga tttagcagcg caggactcgg atattactgt taacacacaa
     6121 atactcgcca acttgccttg gaatatcctc agtcaataca caatagttta ctgttatttc
     6181 ttcaatcatg atgagtacga caggcaatct gactctaaag ccgcagaata tcttgccgaa
     6241 caatttcacg attttcataa tcaaccctac cagcatattc tcttttttgt aggtcatttt
     6301 gaaaaaagtt ttttacgaga aaaacttaaa gctcaactaa atatgggatt ctttttttca
     6361 cccaatatat atgttgcacc tcgtctggca tatagtatta cagacacgag ccgattggaa
     6421 accggaatag agtacacatt tggaaagccc aaagatattg atcttaggag aaacccttca
     6481 aacgataatt tttttataag atttattatg caatattaaa caaaacacaa ggatatagtt
     6541 gatggaaact ttttcgatgg tccggcctga acatctgaat catcatggat ttctttttgg
     6601 tggacagctt cttctctggg tggatgaata cgcatggctt gcggcagccc gagatttacc
     6661 gggatgccgt ctggttacac gagcaatgga ctctattagc tttattcgct ctgctcctgt
     6721 tggttctata cttcgctttg atataagtca aacatataaa gggcaaacta gtgcaaagta
     6781 ccaggtctcc gtattttgtg atccccaaac cggagcccat gaatataata ttttttcgac
     6841 aaatgttatt tttgtaaacg tagaccaaaa ttgtaataaa aaaccatttt gtaacgacaa
     6901 aaattgatga ttagtttaaa cttgaaaggg atttgtattg ggctttatac tttttttctt
     6961 ataggtattt tacatccaat agtaattaaa ggcgagtatt acctaggaaa acgaatatgt
     7021 atattattct ttttctgtgg actgttattt ttaatattct cagttctgat tattgatctg
     7081 tttttttctt gctgtcttgg aataaccggt tttatgcttt tatggtcaat caaggaaact
     7141 tttgatcaag aaaagcgagt tattgcgggt cgctttccaa aaaatcctaa aagaaaatat
     7201 ccgggcaaaa tattatgaaa aggcttacgg tacttaccgg cgcaggaata agcgccgaaa
     7261 gcggtttgaa aacttttcga gacagtaacg gcctttggga agagtacgat gtgatgcagg
     7321 tagcaagtat tgagggttgg aataaagatc ccgaacttgt tttgcaattt tataatgatc
     7381 gacgtaaaca gcttgaattt gtttatccaa acagagccca tttaggactg atggaactgg
     7441 aaaagtattt tgaggtatcg ataatcacgc aaaatgtgga caacttgcat gaaaaagcag
     7501 gaagtagtcg agtccttcat ctgcatggag aacttacaaa ggcttgctct gttgaaaaca
     7561 aaaatcttgt taaagatatt gggtaccgag aaatacttcc cggtgaaaca ggtgaagatg
     7621 gtaaacagct tcgtccatat atagtctggt ttggagaagc ggtaccagct atcggcaaag
     7681 ctattcctct tgtaaaagag gcggattatc tggcagtc
//
LOCUS       DAOTGZ010000134         7705 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_3064
            NODE_11392_length_7705_cov_4.538431, whole genome shotgun sequence.
ACCESSION   DAOTGZ010000134 DAOTGZ010000000
VERSION     DAOTGZ010000134.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868088
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 7705)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7705)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:22:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,785
            CDSs (total)                      :: 2,751
            Genes (coding)                    :: 2,732
            CDSs (with protein)               :: 2,732
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 12 of 19
            Pseudo Genes (internal stop)      :: 5 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7705
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11392_length_7705_cov_4.538431"
                     /isolate="AMR_MDS_3064"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(419..895)
                     /locus_tag="P5123_09185"
     CDS             complement(419..895)
                     /locus_tag="P5123_09185"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRX16476.1"
                     /translation="MDNVDLVKKRAEIEISCKNKMKNGLHWFYYIAALSLVNMILHNT
                     QTDGFFVAGLGITVVVDSVFSNMQNARIIGLIVNIFIISVFVLFGKYSIKSNFIILTG
                     IVLYSLDTLIFFAVQDWFSFGFHIIAIFGLITGYKAKKEWESIQKLDDHNSSLKTQ"
     gene            1244..1708
                     /locus_tag="P5123_09190"
     CDS             1244..1708
                     /locus_tag="P5123_09190"
                     /inference="COORDINATES: protein motif:HMM:NF025879.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="effector binding domain-containing protein"
                     /protein_id="HRX16477.1"
                     /translation="MKYELLFLNKQKYMGIKTKIMFKDHDNIDFARLQKDVINAGIKN
                     IDRNQRFMAIDSDFTKESFSYTPLVAVSEFEGDGFTRYTREEGEYYCFDVNIKDLGPK
                     WFDTCIKFIEQNGFQIDREYDLEYYPEDYLSRIGNNDFLYSEETIQIIFKKK"
     gene            complement(1713..2279)
                     /locus_tag="P5123_09195"
     CDS             complement(1713..2279)
                     /locus_tag="P5123_09195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003468222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcF family protein"
                     /protein_id="HRX16478.1"
                     /translation="MKTKQKKIAIFFALCAAWFSVHVTLTIYDGLSDNIQKCDVAIVL
                     GNKVELDGTPSKRLKARLDRSVQLYHNRLFQYIIVSGGIGKEGFDEAEAMKEYLINKH
                     VPSEFIILDNAGVNSYATALNSKRIMRQQGLKSAMIITQYFHITRTRLAFKKVGLKTY
                     SAHAEYFELRDFFSIFREFFAYYKYLFI"
     gene            complement(2495..3664)
                     /locus_tag="P5123_09200"
     CDS             complement(2495..3664)
                     /locus_tag="P5123_09200"
                     /inference="COORDINATES: protein motif:HMM:NF012760.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016742 - hydroxymethyl-, formyl- and
                     related transferase activity [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formyltransferase family protein"
                     /protein_id="HRX16479.1"
                     /translation="MKTKKNQKNNPLSPLELSMLDKLVVQKRRNKQTMRIVVYLGKSR
                     PRGRVDYGLKLIEQLERDGHYIAGLMVEQDDPIIMYPGVKVKKHIIMPKELCTYLSVI
                     KKNIYENPSFCNKFSEICKKIQNCQADIGVVFFGYWVPPEIYTIPKNGFINYHPGPLP
                     WLRGMEPDTFAILENWKRIWGSVHKVVAGFDAGSIIARTKKAKIELYDTPVSVLNTLT
                     ASGIDTICNVISSFSRKGNFSSVKLPQLGSLATLKKAYDYSFINWQNDTNDMLRRRLL
                     AFCGQDIGIRLKAGFNDLHYVVYNLELHKGKFKGVPGDILGYYICRGKYLLQPIVRTT
                     EGVCVVLLGDKILPERKIPEGHVHSDWIIPPRKQKMVTHRRWIKKSIEVFNKYSN"
     gene            complement(3674..6541)
                     /locus_tag="P5123_09205"
     CDS             complement(3674..6541)
                     /locus_tag="P5123_09205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015183252.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009190 - cyclic nucleotide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0035556 - intracellular signal transduction
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate/guanylate cyclase domain-containing
                     protein"
                     /protein_id="HRX16480.1"
                     /translation="MRKPLRVILILLASLSIVLLLLIYQADQIITRPLDKTEEIKYPS
                     YVIADSLGDIYCIDDSLRRIIKTNRNGEISYIINGGSRNDNSFYNAVALITDNKQNLY
                     VLTRTLDDQGFYVTRERLIKYNALGEFDSVIFETDYSSVERVSQLVQRGRLTGLGSFD
                     EKIRWFEIEAHSVNQYQYDSDTGELDFSRIMAFKNAQIFISYITRLKSGEYVYSTKKG
                     HIAVHDESKKNLDGRFVFVRSAALKGSVSKEDILLNSAGDPVLYSGDNHLSGGMPSVP
                     WYVTAGDNNKIYYSDLTTGSVSRLSYDGTVKEILTNDLLKREGVPDAESIVYTFYVDN
                     SGTIYAALKNSIVVISSNGKTDVVLQSLKRPSQAVISSSVFQLLFLVAFLVSIVFIIR
                     FYTVVMNRRVSIILKQIGIFIPLTVIALLITAKTVFDNMSERYQQELTNRLSLMVQLM
                     TQAVDTQYFNDIKNQSDFMNDAYSHVRESFHLLLNENRDEWNNGYYFALHRVVKGRIY
                     SMMFLNDGISMFHPFDYLNEPGGVYIRALRGEIAAEKAADAWGLWMDAVGPIYSDEGS
                     VVALLEVGKDYTNYAQENARLFRRVLLNVMIISIIFMMLFATLFYLMLTSIRNLRNGA
                     TMIAEGKLEIDIKAKGNDEVADLTISFNNMARAIKKHVSEIVALNTAYFRFVPEQFLT
                     FLNKESVIDVKLGDQIQAEMSVMFTDIRSFTQMSEKMSPKENFDFLNTYLSVVGPVVR
                     ENNGFIDKYIGDAIMALFPQSADDAVQTAVGILKKLHNFNRDLTGEKKEQIAVGFGIH
                     TGLLMLGILGEEQRVDSTVISDNVNLASRLEGLTKKFGASIIISGTTYQALQNRELVL
                     TRYLGRIQVKGKKSATDIYEVLDGLTEEEKVLRIKNREHLETAIEAILAKDLVTARKS
                     LSIAYRINKVDKAVIYYLKLVKSFEKNPELMKSSGIIMKDK"
     gene            complement(6538..>7705)
                     /locus_tag="P5123_09210"
     CDS             complement(6538..>7705)
                     /locus_tag="P5123_09210"
                     /inference="COORDINATES: protein motif:HMM:NF019310.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HRX16481.1"
                     /translation="FLITLAISMSVAFVPVMMRSLYQPLWNLSEDIIIALPISLEMLF
                     FAVGLITGGSLSSRGRWKHAQIAGIALAAAGLLLSALSVNMYIFFAARSFVGLGSGLI
                     LISLRSIVSSEKDSVLKSHAYAHFYAGSMAGLNVGVFFGAFLADQIGFYDVFYVAFAL
                     AIISGLLMVQLLFGIKIAPNKITLKPVGVFRFIIEPKVILFFLFIILPTYTAGMFLVY
                     YFPLFATEKGLSVSDVGRFFIVNGLFVIYLGPTLSRLFKRHFSDRVSLLAGSAGWAIA
                     MAFFALTGNIAGAVATVIVMGIVEGFAVTAQNDYYLNIDLVKKIGEERAVAYFEVAAK
                     IAEMAAPILFGAILILGPLKGIGMFTVLLVIVTLVYIPISAVGHCKDKAGGETK"
BASE COUNT         2490 a         1537 c         1261 g         2417 t
ORIGIN      
        1 catcttcccc tttcaagaga tgggttaccg agtatcctgc accttccaat tgctgttttt
       61 cgaaaatggc tatcagtggc tcatcttcaa caagaagaat gtttttctta tcatttggca
      121 tttatctgtc ccggttacta atattgattc ccgatatcat tttaacagtt tttataaatt
      181 aaccatgagt acgaatatca taatatataa taatcagcat atactttgta attatatagt
      241 aaaaatatga aagtatcaaa aaaaattaac caatgattct gaattacgat ttatttttga
      301 agattttttt tgtatctgta ttaattgtta ttttatcctt cgtatacgcg actaacttat
      361 aatcaaaatt tgtttaatcg atgttatttg taatttatac aattattaga acaagtaatc
      421 actgtgtttt taacgatgaa ttatgatcat ctagcttttg aatcgactcc cattcttttt
      481 tggccttata acctgtgata aggccaaata ttgcaataat atggaaacca aaactaaacc
      541 aatcctgcac tgcaaaaaaa attagagtat ctaatgaata caatacaatc ccggtcaaaa
      601 taatgaaatt cgacttaatt gagtactttc caaataaaac aaaaactgat ataatgaaaa
      661 tattaacaat taacccaatt attcgcgcgt tttgcatatt actaaatacg ctatccacaa
      721 ccactgtaat accaagtccg gcaacaaaaa atccatctgt ctgtgtatta tgcaatatca
      781 tattgaccaa agacaaagca gcaatatagt aaaaccaatg cagtccattt ttcatcttgt
      841 ttttacaact aatttcaatc tcagctcttt ttttgactaa atcaacattg tccatttaaa
      901 gtaccacctt ccatgtattt atgaagaaat aaatcagata tattatcaag tatggcaatt
      961 tatttgcaat tagaaaattt taaacaataa atattaaaat gcatcatact ttatttgata
     1021 gataatccga ataatttctg ctcaaaacat agaacaatca tcctgattat atattctttc
     1081 gaatatctaa taatctacaa ctactaatca aataattttt tatataatat taaactgatt
     1141 gatcatttta catctattct taatagttat caaaacgatc ggatacaatg gtttatttta
     1201 tataaattgt ataattattt cgtaaatatg gagttattaa cagatgaaat atgagctgct
     1261 ttttttgaac aaacagaagt atatggggat caagacaaaa ataatgttta aagaccacga
     1321 caatattgat ttcgccaggc ttcaaaaaga tgtaataaac gccggtatta aaaacattga
     1381 tcgaaaccaa agatttatgg caattgattc ggattttact aaggagagct tcagttatac
     1441 accactggtt gctgtttcag aatttgaagg agatggtttt acaaggtaca ccagagagga
     1501 aggagaatat tactgctttg atgtaaatat taaagatctg gggccaaaat ggtttgatac
     1561 ctgtataaaa tttatcgaac aaaacggctt tcaaatagac agagaatatg atcttgagta
     1621 ttatccggaa gattatctat cccgcatcgg gaataatgat tttttataca gtgaagaaac
     1681 cattcagatc atttttaaaa aaaagtagcc atttatataa aaagatactt ataataagca
     1741 aaaaactctc tgaaaattga aaaaaaatcc cttagttcga aatattcggc atgagctgaa
     1801 tatgttttta acccgacttt tttaaaggcg agtcttgttc ttgttatatg aaaatattgc
     1861 gtaataatca ttgcagattt taatccttgt tgccgcatta ttcttttaga gtttaatgcg
     1921 gttgcataag aatttacccc cgcattatcc aagattataa attccgaggg cacatgttta
     1981 ttaatcaaat attctttcat tgcttctgct tcatcaaagc cttctttgcc tatgcctccg
     2041 cttacaatta tatattggaa taaacggtta tgatacagtt ggaccgacct gtccagtctg
     2101 gctttcaaac gttttgaagg agttccatca agctctactt tgttgcctaa cacaatagct
     2161 acatcacatt tttgtatatt atcacttaat ccatcataaa tagtcaaagt gacatggaca
     2221 gaaaaccaag ccgcgcataa cgcaaaaaat atggcaattt ttttttgctt ggttttcatg
     2281 gatgcgactc atgagcaatt gctattatac catcccaaga ttcaaaagga tgatgtcttt
     2341 ttataaaaat atcattacgc ggctttttgg cattcatcat ttattacagc tgttttttta
     2401 ttatgttttt tcataattga tttaataaat actattataa taatgtcaag tactatgatc
     2461 tataatatgc cgactgatta ttttaatgat aattttaatt agaatatttg ttgaatactt
     2521 caatgctttt ttttatccag cgccgatggg taaccatctt ctgttttcga ggaggaataa
     2581 tccaatcgct atgaacatgc ccttcgggta tctttctttc aggcaatatt ttgtcaccaa
     2641 gaagtacaac acagacaccc tccgttgtgc gaacaatcgg ttgaagaaga tatttgccgc
     2701 ggcagatata atagccaaga atatcgcccg gaaccccctt gaactttcct ttgtgtaact
     2761 caaggttata aactacataa tgcaaatcat tgaaaccagc cttaaggcga atacctatat
     2821 cctgtccgca aaatgccaat agccttcgtc gcagcatatc atttgtatcg ttttgccagt
     2881 tgataaaaga gtagtcataa gcttttttta atgttgctaa agacccaagc tgcggcaatt
     2941 ttactgaaga aaaattgccc ttacgcgaaa acgaactaat tacattgcag atagtatcta
     3001 taccagaagc tgttaatgtg tttaaaacag aaaccggcgt atcgtagagc tctatcttcg
     3061 cctttttggt acgagcaatt atcgagccgg catcaaaccc agctactacc ttgtgaactg
     3121 atccccatat tcttttccaa ttttcaagaa tagcaaaagt atccggttcc ataccgcgta
     3181 accatggtag aggcccagga tgataattaa taaaaccgtt ttttggtata gtatatatct
     3241 ccgggggaac ccagtatcca aaaaaaacca ctccgatatc tgcctggcaa ttttgtattt
     3301 tcttgcaaat ttcagaaaac ttattacaga acgacgggtt ttcataaata ttctttttta
     3361 taacggacaa atatgtacaa agctcctttg gcattataat atgttttttt acttttactc
     3421 cgggatacat tattatcgga tcgtcttgct ctaccataag acccgcaata tagtgcccat
     3481 ctctttcaag ttgttcaata agtttaagtc cataatcgac tcttccacgc ggacgcgact
     3541 tacccaaata taccactatt ctcatggtct gcttgtttct tcgtttttga acaaccagtt
     3601 tatcaagcat actaagctcg agtggagata aagggttgtt tttttgattt ttttttgttt
     3661 tcaatttatt ctcttattta tctttcatta ttattcctga cgatttcatc agttcaggat
     3721 ttttttcaaa cgatttaacc aatttcagat agtaaattac tgccttatct accttattaa
     3781 ttctgtacgc gattgataaa ctttttcgcg ccgttaccag atcttttgca agaatggctt
     3841 caatagccgt ttccagatgt tcacgattct ttatccgtag gaccttctct tcttcggtaa
     3901 gcccgtctaa aacctcgtat atgtctgtcg cgcttttttt gcctttcact tgtattcgac
     3961 ccagatatcg cgtcaatacc agttctctgt tttgcaaagc ctgataagtt gtcccgctga
     4021 ttattatgga agcaccgaac tttttggtaa gcccttcaag acgagaagca agattaacgt
     4081 tatccgaaat aaccgttgaa tctacccgct gttcctcgcc aagtatgcca agcattaata
     4141 gaccagtatg aataccaaag ccaaccgcta tttgctcttt tttttctccc gtcaagtcgc
     4201 gattaaaatt atgaagtttt tttaagattc cgacagcagt ctgtacggca tcatcagctg
     4261 attgcggaaa aagcgccatg atcgcgtcac cgatatactt atcaataaat ccattgtttt
     4321 cacgaacaac cggacccaca acactaagat atgtgttaag aaaatcgaag ttctctttgg
     4381 gactcatctt ctctgacatt tgggtaaatg agcgaatgtc tgtaaacata accgacatct
     4441 cggcctgaat ctgatcacca agtttaacat caataacgct ttctttattt aaaaatgtta
     4501 aaaactgttc gggtacaaaa cgaaaatagg cagtattaag cgcgacaatc tcggatacat
     4561 gcttttttat cgctctggcc atattgttaa aactaattgt taaatcggca acttcatcgt
     4621 ttccctttgc cttgatatca atttccagtt tgccctctgc aatcatggtt gcgccattac
     4681 gtaaatttct tattgaagta agcattaaat agaatagtgt cgcgaagagc atcataaaaa
     4741 ttatgcttat aatcatgaca ttgagtaaca cacgtcgaaa cagacgcgcg ttctcttgag
     4801 cataatttgt atagtcttta ccaacttcta aaagcgcgac gaccgaacct tcatctgaat
     4861 atatcgggcc tacggcatcc atccagagcc cccaggcatc ggcagccttt tcggccgcta
     4921 tctcgccacg aagagctctt atataaacac cacccggttc attgaggtaa tcaaagggat
     4981 gaaacatcga tataccatcg ttaagaaaca tcattgaata gatgcgtcct tttacaacac
     5041 gatgcaaggc aaaatagtac ccattattcc attcgtccct gttttcattt aataacaaat
     5101 ggaagctctc tctgacatga gaatacgcgt cgttcataaa atcactctga ttttttatat
     5161 cattaaaata ttgtgtatct accgcctgtg tcatcagttg caccattaac gacaagcgat
     5221 ttgtcaactc ttgctggtag cgctcggaca tattatcaaa gacggtttta gctgttatta
     5281 atagagctat cacagttaaa ggaataaata ttcctatctg ctttaatata atagatacgc
     5341 ggcgattcat gactactgta taaaacctga taataaatac tattgagaca agaaatgcca
     5401 caagaaaaag caattgaaaa acagatgaac ttattactgc ttgtgaaggc cgtttcaaag
     5461 actgcaaaac aacatcggtt ttgccgttgg aagagatcac aactatactg ttttttaatg
     5521 ctgcataaat agttccgcta ttatcgacat aaaatgtata tacaattgat tcggcgtcgg
     5581 ggacaccctc gcgtttaaga agatcattag tcaatatttc tttaacagtc ccatcgtatg
     5641 ataatcggga aacggaacca gttgtaagat cagaatagta tattttatta ttgtcacctg
     5701 ctgtaacgta ccagggcacc gaaggcatac caccagatag atggttatct ccactgtaaa
     5761 gaaccggatc gccggcactg tttaaaagta tatcttcttt tgacacactc ccctttaatg
     5821 cagcagagcg tacaaataca aagcggccat ccagattttt tttactttca tcatgcacgg
     5881 caatgtgccc ttttttggtg ctataaacat attcaccgct tttgagtctt gttatataag
     5941 aaataaatat ttgcgcgttt ttgaaagcca taattcttga aaagtccaac tcaccggtgt
     6001 ctgagtcata ctgatactga tttacagaat gagcctctat ttcgaaccat ctgatctttt
     6061 catcaaagga tccaagcccg gtaagtcttc cacgttgtac cagctgtgaa acgcgctcaa
     6121 ctgacgagta atctgtttca aaaattactg aatcaaattc acccaaagca ttatatttta
     6181 taagcctttc gcgtgtaacg taaaatccct ggtcatcaag ggttcttgtt aaaacataaa
     6241 gattttgttt gttatcagta ataagtgcta cagcattgta aaacgagtta tcgtttcttg
     6301 agcccccgtt aataatataa ctgatttcac cattacggtt tgttttgatt attcgccgaa
     6361 gtgagtcgtc aatacaatat atatcaccta acgagtccgc tattacataa gaaggatatt
     6421 tgatctcttc tgttttatct agaggacgag ttataatctg gtcggcctga tagattagaa
     6481 gcaacagaac aatgcttaaa gaagctagta atattaaaat tacgcgcaat ggctttctca
     6541 ttttgtttct ccaccagcct tatctttgca gtgaccaacc gcagagatag gaatataaac
     6601 cagtgtcacg ataacgagta agactgtaaa cattccgata ccttttaatg gtcccagtat
     6661 caatatcgca ccaaaaagta taggagctgc catctccgca attttcgcgg caacttcgaa
     6721 ataggcaact gccctctctt cgcctatttt tttaacaaga tcaatattca gatagtaatc
     6781 attttgtgcc gtgacggcaa aaccctctac aatgcccatt actattaccg ttgcaacagc
     6841 cccggcgata tttcctgtaa gtgcgaaaaa ggccattgcg atagcccaac cggcagaacc
     6901 ggcaagaagg gataccctgt cactgaaatg gcgtttaaat aatcttgata atgtcggccc
     6961 aagatagata acaaaaagcc cgttaacaat aaaaaaacga cccacatctg ataccgaaag
     7021 ccctttttcg gtagcaaaaa gagggaaata gtataccaag aacattcctg ctgtatatgt
     7081 aggtaatatt ataaaaagga aaaagagaat aaccttgggt tcaataataa agcggaatac
     7141 cccaacaggt tttagagtta ttttattagg tgcaattttt ataccgaaaa gcagttgaac
     7201 catcaataaa ccactgatga tcgcaagcgc aaatgcaaca taaaagacat cataaaaacc
     7261 aatttgatcg gcaagaaagg ctccaaagaa aacacctacg ttaaggccag ccattgaccc
     7321 cgcgtaaaaa tgggcatagg catgcgattt taaaacgctg tctttttcgc tgcttactat
     7381 ggaacgcaaa ctgataagta ttaatccgct tccaagacct acaaacgatc ttgccgcaaa
     7441 gaagatatac atattaacag agagagcaga gagcagcaga cccgcggcag caagcgcaat
     7501 cccggcaatc tgggcatgct tccaccttcc ccgtgaagaa agcgaaccgc ctgtgatcag
     7561 ccccaccgca aaaaagagca tttctaaaga gattggcaaa gctataatga tatcctctga
     7621 caggttccat aacggctgat atagagaccg catcattacc ggcacaaaag ctaccgacat
     7681 agatatggca agcgttatca aaaag
//
LOCUS       DAOTGZ010000135         7605 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_3064
            NODE_11574_length_7605_cov_5.436026, whole genome shotgun sequence.
ACCESSION   DAOTGZ010000135 DAOTGZ010000000
VERSION     DAOTGZ010000135.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868088
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 7605)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7605)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:22:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,785
            CDSs (total)                      :: 2,751
            Genes (coding)                    :: 2,732
            CDSs (with protein)               :: 2,732
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 12 of 19
            Pseudo Genes (internal stop)      :: 5 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7605
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11574_length_7605_cov_5.436026"
                     /isolate="AMR_MDS_3064"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(149..1384)
                     /locus_tag="P5123_09215"
     CDS             complement(149..1384)
                     /locus_tag="P5123_09215"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HRX16482.1"
                     /translation="MNILIVEDKQEARYLLEVLLKSRGYVTVSASNGREGLQKLDNNK
                     IDLIISDILMPEMDGFKFCTLVKSDKSKAGIPFVFYTATYVDKRDEDFAMRLGADLFL
                     RKPLEPDVLLSEIDKIIERVNQGGYDSPQVKLEMGSDDKEVYKLYSERLVKKLEDKVD
                     QLEKEVVKRQDAEVKLQKMVSEKEILIKELYHRTKNNMQVISSMLWLYSNKILNEEWG
                     GIVKNVNLKIRTMALVHQELYESSDLSHVNLKEFFKNLVVLLQKNYLPDGKVCEINTD
                     LQDIRVLLDTAIPLGFVLNELITNALKHAFLPDSVEMQGGLTEKSSEHLEHNGRLENH
                     RIDIKLYKGKEGGIVLEVADNGGGLPKDFDFDVESHPGLLLMRDMVVSQLKGKVKYKT
                     DNGLIWTVVIDHECYDERV"
     gene            complement(1403..1780)
                     /locus_tag="P5123_09220"
     CDS             complement(1403..1780)
                     /locus_tag="P5123_09220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020181594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HRX16483.1"
                     /translation="MKRVLVIEDNDANLYLISYILMSNGIDVISCKEGLPGVETALLE
                     QPDLIIMDIQLPDINGLEATRRIREDERGKDLVIVALTSYAMTGDREKALAAGCTGYM
                     EKPINPERFILEINSYLGSEKNL"
     gene            complement(1758..3926)
                     /locus_tag="P5123_09225"
     CDS             complement(1758..3926)
                     /locus_tag="P5123_09225"
                     /inference="COORDINATES: protein motif:HMM:NF012707.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HRX16484.1"
                     /translation="MIKSIKITLLLFVALNSVAIMAQNMDRPLRVGLYNNYPKIYRND
                     EGKPDGVFPSILKQIADAEGWQLEYRFGTWDECLGMLERGEIDIMPDVAVSEARKIIF
                     DFHQKPVLTSWGCIYTKSRLDINSVLDLHGRTVAVLRGSILTDGEDGIYNLVKRYDVE
                     CSFIEADDYNQVFDLVRTGKADAGVVNRFFGAAFDDEYGLVLNNFIFYPTALGFAFPK
                     GASLNTILSDRIDHHLDLWHTDMDSVYYHILTEHKLYPKKKMPVWVVPLIAGLAGLII
                     LFIILSILLKWQVNRKTSLLQHINKKLSDEIDEHHVTMALLEKSRELYRSFAENIPGL
                     VFMYDKDSNGNRKSLIKGKREEEFLGSRVGKKTFADVNNFFEYVEPEDRRIIEELSQK
                     AQAGNGILDVEYRVNIDKDKTKWFRTIGRVTALANGNYRWQGVMLDIDDRKKLERQLD
                     LHRKYLEELVDSRTQDLQLRTEELEKANKNLKEADRLKSIFLASMSHELRTPLNSIIG
                     FTGILLMGMVGELADEQRKQLEIIKKSSNHLLALINEILDISKIEANKAELSLEKIDI
                     KDAVQESLSILNNRVSEKNIKLDYVCEENFTVLSDRRRISQIVINIAGNAIKFTDKGG
                     VTVTVKKINNKFFEISVIDTGCGVKDEDMQKLFEPFQQIDSSLTKKHEGTGLGLHLSQ
                     RIVVMLGGSIRVESEYGNGTSFFVQLPIETEVKIEKSSGD"
     gene            complement(4286..4777)
                     /locus_tag="P5123_09230"
     CDS             complement(4286..4777)
                     /locus_tag="P5123_09230"
                     /inference="COORDINATES: protein motif:HMM:NF012792.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HRX16485.1"
                     /translation="MIKLVNAKADQIARLVDASIAAFESDVEFGAPAGESGPPGFNSI
                     GWHKNMLSCSDGFFSFYVGDNLIGGALVFRDRNRTKKQYNLGRIWIDPEFQHQGYGLK
                     AMELILDKFPDAECWTLETPSWNIRTRNFYLKAGFTCIKEARGELHFVKKMASEQTQQ
                     PAL"
     gene            complement(5214..6566)
                     /locus_tag="P5123_09235"
     CDS             complement(5214..6566)
                     /locus_tag="P5123_09235"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRX16486.1"
                     /translation="MNKIIRSLTWVGLIFLLCFLVACGGDDGKSENDNIPLKTIDITN
                     AQNLFVTADISSRSDEKPTLYKITADGKVEKVSYFDENGKELSCVPNQDGYCDDSSPV
                     YIQNINNNYIVILFGWPSNADYPEFGYLVRKSDGAVFGLGDAVPTGQGGNGFINGKKI
                     QTDSSGNLYFIYYSYDYEKDCLRKINIEDPNKITSERYTPDIDNVHFFTVDSNGNVIY
                     SASLSSSDGSVYYSRIKKTNGGIKDILYDYFAHWKGLDGSIYLSGWDNNNTIEKVTVD
                     GAYNVTFSDYITNFYTTINSTDYYMTCGSAGSYLFKTADKIVIYETDYPVVMWEVDNG
                     SNSPRAIDISAADISSVISATCSDNYYYIVGKSSGSVNFIKIDPTDGTTTTLLSSDLY
                     YVTCFSVSSSNQITFNALRYSDGAKIIGKIDANGSGPYIVTIVDESINAEISVLERIN
                     "
     gene            complement(7300..7527)
                     /locus_tag="P5123_09240"
     CDS             complement(7300..7527)
                     /locus_tag="P5123_09240"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRX16487.1"
                     /translation="MDLLYAVADKIITIEIKAAGTFREEFFKGLDYFDSAISPALKKI
                     VVYDGDRDEERSRGIVTNFNSVRERLIADQL"
BASE COUNT         2315 a         1633 c         1178 g         2479 t
ORIGIN      
        1 atctacttga tacaaatatt ataatcggat ttcttaaaaa tgatccaacg attgtacaaa
       61 aaattagcaa tctataaaga ataactctac caataattac tctcggcgaa atgctttatg
      121 gggcaaattg ttccaataat ctgctcgatt acactcgttc gtcatagcac tcgtgatcaa
      181 ttaccacagt ccatataagt ccattgtccg ttttatattt aaccttgcct ttaagctggc
      241 taacaaccat atcccgcatt aaaagcagcc cagggtgaga ctccacatca aaatcaaaat
      301 ctttcggcag ccccccaccg ttatcggcaa cttcaagcac aatgcctccc tcttttccct
      361 tatacaactt gatatcaatt ctatgatttt ctaaacgtcc gttatgttcc aaatgctcgc
      421 tactcttttc cgttaagccc ccctgcatct ccacgctatc cggcaaaaac gcatgcttga
      481 gtgcgtttgt tataagctcg tttaaaacaa accccagagg tattgccgta tcaagaagaa
      541 cccttatatc ttgcagatcc gtatttattt cgcacacttt accgtcgggt aaatagtttt
      601 tttgtagaag aaccacaaga tttttaaaaa actccttaag gttcacatgc gaaagatcgc
      661 ttgactcata aagctcttga tgtaccagag ccatggtacg tatcttaaga ttcacattct
      721 tgacaatacc accccactct tcattaagga tcttgttaga atagagccaa agcatggagc
      781 taattacctg catattattt ttagttcggt gatacagctc ttttattaga atctcttttt
      841 cagataccat tttttgaagc ttgacttctg catcctgtcg cttaacaacc tctttttcaa
      901 gctggtcaac cttatcttca agttttttta caagcctttc gctatataat ttataaacct
      961 ctttgtcatc actgcccatc tcaagcttta cctgcggcga atcgtaacca ccctgattta
     1021 ctcgctcaat tatcttatca atttcactca gtaaaacatc gggctcaagc ggtttgcgca
     1081 aaaaaagatc cgctccaaga cgcatggcaa aatcctcgtc tcttttatct acatatgttg
     1141 ctgtataaaa aacaaagggt atacctgctt tactcttatc acttttaaca agcgtgcaaa
     1201 acttaaaacc gtccatctcg ggcattagaa tatcagatat tatcagatca attttattat
     1261 tatcaagttt ttgcaacccc tctctgccgt tcgaggcaga cacggtaaca taacctcggc
     1321 tttttagcag tacttcaagc agatatcttg cctcttgttt gtcttcaact atcagaatat
     1381 tcattaaatc tccatacgtc tcttacagat ttttttctga ccccagataa ctatttatct
     1441 ctaaaatgaa cctttcaggg tttatcggtt tttccatgta tcctgtgcag cctgcagcta
     1501 atgctttctc gcggtcaccg gtcattgcgt aagacgttaa agccactatt acaagatcct
     1561 ttccacgctc atcttctctt atacgtcgag ttgcctccag cccattaata tcgggcaact
     1621 ggatatccat aataatcaag tcaggttgct ctaacaacgc ggtttcaact cctggcaagc
     1681 cctctttaca cgatattaca tcaattccgt tactcatcag aatataactt atcagataaa
     1741 gatttgcgtc attatcttca atcaccagaa ctcttttcaa ttttcacctc cgtctcaatt
     1801 ggcagctgta caaaaaaact tgttccattt ccgtattcac tctccacacg gatacttccg
     1861 ccaagcataa caacaatcct ctgggacaaa tgaagcccaa gtccagtgcc ttcatgtttt
     1921 tttgtaagtg acgaatctat ctgttgaaac ggctcaaaca gtttttgcat atcttcatcc
     1981 tttacaccac agcctgtatc tataaccgaa atttcaaaga atttattatt aatttttttt
     2041 actgtaacag taacgcctcc tttatcagta aacttgatag cgttacccgc aatattaatt
     2101 actatctgac ttattcttct tcggtcacta agcacggtaa aattctcttc acatacataa
     2161 tcaagtttta tatttttttc agaaactcga ttattcaata tactcaaact ctcttgaact
     2221 gcgtctttaa tatcaatttt ttcgagagac agctctgctt tgttagcttc tattttggat
     2281 atatcaagta tctcgttaat caaagccaaa agatggttcg agcttttctt gattatctca
     2341 agctgtttac gctgttcatc ggcaagctca cccaccatac ccataagcaa aatccctgta
     2401 aaaccgataa tcgaatttaa cggcgttcta agctcatggc tcatacttgc caaaaagatt
     2461 gattttaatc tatctgcttc tttaaggttt ttgttcgctt tctctagctc ttcagttcgc
     2521 aactgcaaat cctgtgtacg gctatctacc agctcttcaa gatatttacg gtgaagatcg
     2581 agctgacgct ccagtttttt gcgatcatca atatccagca ttaccccttg ccatcggtaa
     2641 ttaccgttag caagagccgt aaccctaccg atagtgcgaa accattttgt cttgtcttta
     2701 tcaatattta ctctgtactc aacgtccaaa ataccgttac ccgcttgggc tttttgtgac
     2761 agctcttcaa ttatccgtct gtcttctggt tccacatatt caaaaaaatt gttaacatca
     2821 gcaaatgttt ttttgccaac tcttgaacca agaaactctt cttcccgttt acctttgata
     2881 agcgattttc tattgccatt agaatccttg tcgtacataa agacaaggcc aggtatattt
     2941 tcagcaaaac tgcggtaaag ctcgcgactc ttttcaagaa gagccattgt tacatgatgt
     3001 tcgtctatct cgtcagaaag tttcttatta atatgttgca gcaaactagt ctttctattt
     3061 acctgccact ttaacaaaat agacaagata ataaatagta taatcaaacc ggcaagacca
     3121 gcaatcagag gcactaccca tacaggcatc ttcttttttg gatacagctt atgctcggtt
     3181 aatatatgat agtagacaga gtccatgtcc gtatgccaga gatcaagatg atgatcgatt
     3241 ctatcagaaa gaatcgtgtt taaagaagca ccctttggaa aagcaaaacc aagcgctgtt
     3301 gggtaaaata taaaattgtt tagtacaagc ccatactcat catcaaatgc ggctccaaaa
     3361 aatcggttaa caactccagc atcggccttg cctgtgcgca caagatcaaa aacctggtta
     3421 taatcatcgg cttcgataaa actacactct acgtcgtagc gcttaaccag attgtatata
     3481 ccatcctcac catcagtcaa aatacttcct ctaagcacag caacggttct tccatgtaga
     3541 tcaagtaccg aattaatatc aagtcgtgat tttgtataga tacatcccca gcttgttaag
     3601 accggctttt ggtgaaaatc aaaaattatt tttcttgctt cagatactgc tacatcgggc
     3661 attatatcta tttcgcctct ttcaagcatc ccaagacact catcccacgt accaaaccgg
     3721 tactcaagct gccaaccttc ggcatcagca atctgtttta atattgatgg aaacaccccg
     3781 tccggcttgc cctcatcgtt acggtaaatt tttggatagt tgttatacag gccaactcgc
     3841 agggggcgat ccatattctg tgccataatt gcaacagaat ttagagctac aaataaaagc
     3901 agggttattt ttatgctctt tatcacagtt tattatcccc gtgtcataaa tgacgatcca
     3961 gacatataca aattactgaa atttgtcttg ttttcaataa aaattacaaa tgaatcactc
     4021 aatagtctgt atccaaaatt aaccttaact attcaacgca ggtaatgcaa agaaatgaga
     4081 tccgaagttt acaacattag gatagaaaga agttataaat attaaccaaa tgagattatc
     4141 agtaacaaca cggcaccaca gtattaatga aaagcagtac cgagttgctc acagtctgtt
     4201 tccagatttt ctgatttaag tctgttgtat aattttcagt gattctatct agattatatg
     4261 atgttgcata agcaataacg gatgtttaaa gagccggttg ctgggtttgt tctgaagcca
     4321 ttttttttac aaaatgtaac tcacccctgg cttcttttat gcatgtaaaa ccggctttaa
     4381 gataaaaatt acgtgtgcgt atgttccaag aaggtgtttc cagtgtccaa cactctgcat
     4441 ctgggaactt gtccagtata agttccatcg ccttaagacc atatccttga tgctgaaact
     4501 ctggatcaat ccagatgcgc cctaggttgt actgcttttt tgttcgattt cggtcacgaa
     4561 acacaagggc tccaccaatc aaattatcac caacataaaa agaaaaaaac ccatccgagc
     4621 acgagagcat atttttatgc cagcctatgc tgttaaaccc cggaggacca ctttcgccgg
     4681 caggcgcgcc aaactctaca tcagattcaa aagccgctat gctcgcgtca acaagccgcg
     4741 caatctgatc cgcttttgcg ttaacaagtt taatcatttt cacatacctc attattaaag
     4801 tatgcttttg tatcatatat ccggttcaaa tacacgtccg gttttgctat ttatgggaat
     4861 ttttaaaaaa acttaggtaa attaccttaa atcaaaagtt gtgtgaaagt gtctcaccaa
     4921 tgtgacaaac gggagggtgt ccggttcgct atttatatac tttgctattt ttttcgaggt
     4981 gtcatctgtt ttagataaaa gattaggact ctttatcttt actttatagt aaagtacgca
     5041 ccataagata ccgtgctaca agggaaaatt cttgtgaaaa gacataagct cgtatattgg
     5101 gagtaaatga aagaatctgt tgacagaaag ccccctgcat catggtggat taatttaaat
     5161 cattgcagcc cctcattctt aaggggctgc aatgattatg gattaaaaca gtatcagttt
     5221 attctttcta aaacagagat ctctgcgtta atggattcat caacaatagt aactatataa
     5281 ggtccgcttc catttgcatc tattttgcca attattttag caccgtctga ataacgaaga
     5341 gcattaaaag tgatttggtt ggagctgctg actgaaaaac aggttacata atagagatcg
     5401 gaggagagca atgttgtagt tgtaccatct gtgggatcaa tttttataaa atttacgctt
     5461 ccagaacttt taccgacaat atagtaataa ttatctgaac atgtagccga tataacagat
     5521 gaaatgtcgg ctgctgatat atctattgcc cgtggagaat tggaaccgtt atcaacttcc
     5581 cacataacaa caggataatc tgtttcataa attacaattt tatctgctgt tttaaaaaga
     5641 taagagccag cactgccaca tgtcatataa tagtctgtac tgttgatcgt tgtataaaaa
     5701 tttgtgatgt aatcagaaaa ggttacgttg taagctccgt caacagtaac tttttcaata
     5761 gtattgttgt tatcccatcc tgaaaggtaa atactgccgt ccagcccttt ccagtgagca
     5821 aaataatcat ataagatgtc ttttatacca ccattggttt tttttatacg gctataatac
     5881 accgatccat ccgatgatga aagagatgca gaatatataa cattaccatt agagtcaact
     5941 gtaaagaaat gaacattgtc aatatccgga gtatatctct cactggtaat tttgttagga
     6001 tcttcaatat ttatttttct taaacaatct ttttcatagt catatgaata atatataaaa
     6061 tataaatttc cagatgagtc ggtttggatt tttttaccat ttataaaccc atttccgcct
     6121 tgtccagtcg gaacggcatc tcccaggcca aatacagctc catcagattt cctgaccagg
     6181 taaccaaatt ccggataatc agcattactg ggccaaccaa agagaattac aatataattg
     6241 ttatttatat tctggatata aacagggcta ctatcatcac aatagccatc ttgattaggc
     6301 acacaagata attcttttcc gttttcatca aaatagctga ctttctcgac ttttccatca
     6361 gcagtaattt tatagagtgt aggtttttca tctgatctgc ttgaaatatc tgctgtaaca
     6421 aagagattct gggcgtttgt aatatcaatt gtttttagtg gaatattatc gttttcgctt
     6481 ttaccatcat cgccaccaca agcgactaaa aaacacaaaa gaaagattaa accgacccaa
     6541 gttagagacc tgataatttt attcataatc accaccacca ccaccatatt acatttttac
     6601 ataattttac gtttaaaaag cgtctatttt taatatacga tcaaataatt gatgatcata
     6661 attttaatca tgcaaatata tagcacatgc gggcttttta tgtcaattgg ccgattggcc
     6721 agtatttgta aaaaatttgt taaataaatg ttaattattg ccaatttctt gaatatcctg
     6781 caaaaaatta taaaagtact ctctccctct ttcctgctac aataagtgtt atacttgttt
     6841 ttgaccacat ttataacttt aatggaactg atcccacttt gacttcatca attaatgtat
     6901 tatttttgtt atataaacca ataacattat aactgctgct aaatgatgaa ttattctata
     6961 ttggtacata tataagaaat tcttcattct cggatcatcc aagaataact ggctcttcta
     7021 aataattact attgttcata aaatatttta tattatatta tagaaaactt cgaaaaatca
     7081 ccattttagg aaaggctata ccttttaaag ggatattaat gacaattttt atcgtagcca
     7141 ctcgaaaaac cgctcataaa tatcgctttg aggtcatcgt tatttatggc tttatataag
     7201 gaatttttcg caagtttctt attaaaaagt tacttatcag agcttgcctg tattctttta
     7261 ggagattatt actacatgaa attaacaaat aatcacactt tacaactgat ccgcgattag
     7321 tcgttccctt acactattaa aattggttac tataccacga cttcgctctt catcacgatc
     7381 accgtcgtac acaacgattt tttttaatgc cgggcttatg gccgagtcaa aatagtccag
     7441 tcccttaaaa aattcttctc taaacgtgcc ggctgcctta atctcgatcg ttataatctt
     7501 gtccgcaaca gcataaagaa gatccacctc atggcctttg gcgtcacgac aagaaagagg
     7561 ttgtttgttt taacattatt aaaactagtc ttgagtattt ctgtt
//
LOCUS       DAOTGZ010000136         7461 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_3064
            NODE_11874_length_7461_cov_4.074264, whole genome shotgun sequence.
ACCESSION   DAOTGZ010000136 DAOTGZ010000000
VERSION     DAOTGZ010000136.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868088
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 7461)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7461)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:22:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,785
            CDSs (total)                      :: 2,751
            Genes (coding)                    :: 2,732
            CDSs (with protein)               :: 2,732
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 12 of 19
            Pseudo Genes (internal stop)      :: 5 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7461
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11874_length_7461_cov_4.074264"
                     /isolate="AMR_MDS_3064"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..372)
                     /locus_tag="P5123_09245"
     CDS             complement(<1..372)
                     /locus_tag="P5123_09245"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025033.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HRX16488.1"
                     /translation="MFKELQKINKRPALYSKYTAQELWDDEHISSQMLETHLDDSIDL
                     ASRSGDFMACSAKWIIEHFKLGSGVSVADFGCGPGLYAEKFSSCGADVFGIDFSRRSI
                     TYAKEVAKKNKSNIEYQCGNYL"
     gene            complement(651..1400)
                     /gene="trpA"
                     /locus_tag="P5123_09250"
     CDS             complement(651..1400)
                     /gene="trpA"
                     /locus_tag="P5123_09250"
                     /EC_number="4.2.1.20"
                     /inference="COORDINATES: protein motif:HMM:TIGR00262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004834 - tryptophan synthase activity
                     [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan synthase subunit alpha"
                     /protein_id="HRX16489.1"
                     /translation="MSTYNGIYLVGNYPDKDTFIKAALKGLELFDFLEVGIPFSDPVA
                     DGPVIVDAAFKALENGETMDSILDSVDVIREQMPSEKKIYFMNYANTAHAPGYSNFCK
                     KCKDHSIAGMIVPDIPFLESVDLKKVATDSGLDYINFITPESTLEQIEEVVTGATGFV
                     YAISMRGITGQDLAFDDATIQKIKHARLKSNVPVVIGFGIRDAAAAKSALNIADGFIM
                     GTEPVMRLGKNFDEFAKFIDELSSKLKTVNK"
     gene            complement(1397..2557)
                     /gene="trpB"
                     /locus_tag="P5123_09255"
     CDS             complement(1397..2557)
                     /gene="trpB"
                     /locus_tag="P5123_09255"
                     /EC_number="4.2.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013682550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004834 - tryptophan synthase activity
                     [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan synthase subunit beta"
                     /protein_id="HRX16490.1"
                     /translation="MRGYFGEFGGRFVPETLINVLNELEKSFESFQKSEEVKTELADL
                     LENYAGRPTPLYFAKRLSEKTGVRIYLKREDLLHTGAHKLNNTLGQIMLARFMNKKRI
                     IAETGAGQHGVATATVCALMGLECVVYMGAVDIERQATNVKRMKLLGATVVPVNSGSK
                     TLKDAVNEALRDWVKSCQNTHYILGSVTGPHPFPKIVREFQKIIGEETIRQFKLKEGR
                     LPDAVFACVGGGSNAAGMFYPFAETDVPLYGVEAGGRSHNPGDHCETLTQGSPGIFQG
                     ARSYLIQDGYGQVLPVHSVSAGLDYPSVGPEHSYWKDANRVTYVSCNDEDALAACDTL
                     TKLEGILPALESSHAVGYLLQNTSTFKDKTVVINLSGRGDKDLGIILGEVQL"
     gene            complement(2575..3180)
                     /locus_tag="P5123_09260"
     CDS             complement(2575..3180)
                     /locus_tag="P5123_09260"
                     /inference="COORDINATES: protein motif:HMM:NF012900.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylanthranilate isomerase"
                     /protein_id="HRX16491.1"
                     /translation="MFCKYCGFTRKEDVDFAVSLRVDAIGLIFYPGSKRYIDASTAAK
                     ITSRVPSSIMKVGIFVNESINVINDVSKSAGLDYVQLFAAEKTKAARIEYPVLLSYRI
                     ENSLDFEKIDCKGPFLLDALSLKGEGGTGESFDWTLLKDFKLISKTIIAGGVNCDNLD
                     KLFQIAKPYGVDLSSGLEDSPGIKSHQKMEQFKKRLEELTK"
     gene            complement(3170..3931)
                     /locus_tag="P5123_09265"
     CDS             complement(3170..3931)
                     /locus_tag="P5123_09265"
                     /inference="COORDINATES: protein motif:HMM:NF012444.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="indole-3-glycerol-phosphate synthase"
                     /protein_id="HRX16492.1"
                     /translation="MTKFKLNKMREIKNSEQDNVKQLLNYSDSRFRPAYPFIPSDAID
                     IIAEVKRSSPTQTLDTSAKAASQAIAYQNGGTAAISVLTDSNFFSGSFEDLRDVCNAV
                     TVPVLCKEFIYFKEQIELAYRYGADMVLLIAQSLTDYELTSLYEYAMERSVVPVVEIN
                     RIEELDRVMAINPQIVMVNMRDLNTLVIDCNAGSKVLKQVPKSVLRISASGINTSDDV
                     KKISTDTGVTVFLIGSALMKSGDPSCFVKELSSVL"
     gene            complement(3936..4940)
                     /gene="trpD"
                     /locus_tag="P5123_09270"
     CDS             complement(3936..4940)
                     /gene="trpD"
                     /locus_tag="P5123_09270"
                     /EC_number="2.4.2.18"
                     /inference="COORDINATES: protein motif:HMM:TIGR01245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004048 - anthranilate
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anthranilate phosphoribosyltransferase"
                     /protein_id="HRX16493.1"
                     /translation="MNTKEMLKKVYDGGVLNKIETTTLFNSLFQGEVAECELAALLIA
                     MKMRKESADEIAGAAVSMREFALSPGITGDNLFDTCGTGGDGIHSFNVSSAVAIILNS
                     MGCPIVKHGNRSVSSLSGSADFYEALGIPINLKAEEARTYFDKTNFIFMFAPNYHPAM
                     KYAVPVRKQLATRTLFNMLGPLTNPTRPKRQMIGIFDKAYLPVYSQAAVQIGFDHLVL
                     YSSQDGMDEVSPFAITQVAEIKGHNIREYEIDPAPYITKEEAKSIPSHYSAQQNAQLF
                     LETVSEKKDTPLVKLLSLNSALALCVLDKASSIEEGFKMAYEQIISGNVMHSIKRLRG
                     "
     gene            complement(4918..5499)
                     /locus_tag="P5123_09275"
     CDS             complement(4918..5499)
                     /locus_tag="P5123_09275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012357237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminodeoxychorismate/anthranilate synthase
                     component II"
                     /protein_id="HRX16494.1"
                     /translation="MICVIDNYDSFTYNVVNYIKKCGEDVQIFSNDCDFSLIDFSRYN
                     GILLSPGPSTPDNSGITLDVLKTITYLPVLGICLGMQAVGLVFGGKVVHAAKTMHGKI
                     DSVKHSGGRLFADIPDSFKVVRYHSLAVSSDELPSTLKITALSSDNEIMAIEHNTLPI
                     YGVQFHPESYLSEHGMKIIKNFTEICHEYKRDA"
     gene            complement(5508..6983)
                     /locus_tag="P5123_09280"
     CDS             complement(5508..6983)
                     /locus_tag="P5123_09280"
                     /inference="COORDINATES: protein motif:HMM:NF012640.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anthranilate synthase component I family
                     protein"
                     /protein_id="HRX16495.1"
                     /translation="MHYPDFNEYQKLAKQYSRIPVYRELNLEEPGHLLILKVMSEAGD
                     VMFLESLGDNSDNTRFSFMGINPRHHYRFESGQMYKDNVHTGSFPEDKVFGYLKKEID
                     RYTSPAFEQFGHFNGGLAGYFGYEMVNYCGILRKPVRENNKIPQIYLMHIDEFICFDN
                     ETKKYYLSVCVYPEDGVQKSYYNALARLEELEEEIINKISETTLPYMPSRAEEVEPRF
                     TESKTNFMSTVAKAVSMIDEGEALQIVLSMRAMIPEDIDPYRFYLRLRKTNPSPYMYF
                     IKVNGIVIVGSSPEVHVRCSGREAMLRPIAGTAKRTGDEKADKLATEAMLADEKECAE
                     HLMLVDLARNDLSRIAIPESVKVTRFMKPENYSHVIHLVSDVESVLDEDKHMIDVLKE
                     SFPAGTVSGAPKVRAIEIIDECETQPRDIYSGAIGYMGFNQYLDTCITIRTAYFTKEG
                     NYLQAGAGIVQDSKPENEYQEIRNKLGALASSLAYAMPRKV"
     gene            7280..>7461
                     /locus_tag="P5123_09285"
     CDS             7280..>7461
                     /locus_tag="P5123_09285"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRX16496.1"
                     /translation="MARKEKLDKKPEKRRTGEQENTGGNGKTRIVRNRENGDGDVFPR
                     NGDGDAFPRNGDGDFFP"
BASE COUNT         2259 a         1624 c         1298 g         2280 t
ORIGIN      
        1 tagatagtta ccacattgat actcaatatt acttttattt ttttttgcca cctctttagc
       61 gtacgtaata gaacgtcgag aaaaatcgat accaaaaaca tccgcaccgc aacttgaaaa
      121 tttttctgca tataaacccg gaccacagcc aaaatcggct acagatacac cagagccaag
      181 tttaaaatgt tcgataatcc attttgctga acaagccata aaatcgccgc ttcttgatgc
      241 aagatctatt gaatcatcaa gatgagtttc caacatttga gacgatatat gttcgtcatc
      301 ccaaagctct tgagcggtat atttactgta taaggcgggg cgtttgttaa ttttctgtaa
      361 ctctttaaac ataatattta ctcactcgct taattctctg attttttttg ctaagcctca
      421 tatggtaaaa ctttttgtca aaactatata tccacaataa ttactaaagt taaaatcgcg
      481 gttagatagt gccaatccaa agatatttta ccataatttt tatattttat agaaaaaaaa
      541 tctgctcttg tagaacagat tttataattt acagcaaaat attgctgtca tattttttct
      601 ttgataaagt taagcttaca gatttttcgc tgagaatcat atttaactaa ctatttattt
      661 acagttttca acttagacga aagctcatca ataaactttg cgaactcatc gaaatttttt
      721 ccaagcctca taactggctc tgttcccata ataaatccat cagctatatt caaggccgac
      781 ttagctgctg ccgcatctcg tatgccaaat cctataacaa caggaacatt actctttaat
      841 cgggcatgct tgattttttg tattgtagca tcgtcaaaag cgagatcttg tcccgtaata
      901 ccacgcattg atattgcgta tacaaatcct gtggctccag ttacaacctc ttcgatttgt
      961 tctaaagtac tctcaggtgt aatgaaatta atgtaatcaa gaccggaatc agttgctact
     1021 ttttttagat ctactgattc taaaaatggt atgtctggca ctatcatacc tgcaattgag
     1081 tgatctttgc attttttaca aaaattagag taaccaggtg catgtgccgt gttagcataa
     1141 ttcataaaat atattttttt ctcggacggc atttgttcgc gaatcacgtc caccgaatca
     1201 agaatagaat ccatggtttc accattttca agagccttga aagcagcatc cacaatgacc
     1261 ggtccatcgg caacaggatc agaaaagggt atgcccactt caagaaaatc aaatagctcc
     1321 aagcccttta acgcggcttt tataaaagta tctttatcgg gataattacc aacaagatat
     1381 attccattat aagtgctcat aattgtacct cgccgagtat tattcctaaa tccttgtctc
     1441 cccttccgga aagatttatt acaacagttt tgtctttaaa agtggacgtg ttttgtagca
     1501 gatagccaac cgcgtgactg ctttcaagtg cggggagtat gccctcaagt tttgttagtg
     1561 tatcgcatgc agcaagcgca tcttcatcgt tacacgacac gtatgtaaca cggtttgcgt
     1621 ccttccaata tgagtgttcc ggacctactg atggataatc aagccctgcc gatactgagt
     1681 gcacaggaag aacctgacca tagccatcct gaataaggta agatcgcgca ccttgaaaga
     1741 taccgggaga cccctgagta agtgtttcac agtgatcacc tggattatgt gatcggcctc
     1801 ctgcctctac accataaaga ggaacatcag tttcagcaaa agggtaaaac atacctgcgg
     1861 cattacttcc tccacccaca caggcaaata cggcatccgg cagacgcccc tctttcagtt
     1921 taaattgacg aattgtctct tctccaatta ttttttgaaa ctctcgcaca atcttaggaa
     1981 acggatgagg tccagtaacc gagccaagaa tatagtgtgt attttggcaa cttttaaccc
     2041 aatcacgcaa agcctcatta acagcatctt taagggtttt agaccccgaa ttcacaggaa
     2101 caaccgtggc tcctaaaagt ttcattcttt taacattggt tgcctgacgt tctatatcga
     2161 cagcccccat ataaacaaca cattcaagcc ccataagagc acataccgtt gcagtggcaa
     2221 ctccatgttg ccccgcacct gtttcggcga tgattctttt cttattcata aaccgagcaa
     2281 gcataatctg accaagtgta ttgttaagct tgtgagcacc tgtatgcaga aggtcttcac
     2341 gtttaaggta tatgcgcaca ccggtttttt ctgataatct ttttgcgaaa taaagcggag
     2401 ttggtcgtcc ggcataattt tccaaaagat ctgctagttc agtcttaact tcttcacttt
     2461 tttggaaact ctcaaaagat ttttccagtt cattaagaac atttatcaga gtttccggga
     2521 caaaccgtcc accaaactct ccaaaatatc ctctcatgtt aggcaaaata ttactcattt
     2581 agttaattcc tccagtcttt tcttaaactg ttccattttt tgatgggatt ttattcctgg
     2641 actatcctct aaaccagacg agaggtctac cccatacggt ttagcaatct gaaataactt
     2701 atccaaattg tcacaattca caccacccgc gattattgtt ttacttatta atttaaaatc
     2761 ttttaaaagc gtccaatcaa atgactctcc agtaccacct tcacccttta acgaaagtgc
     2821 atctaataaa aatggtcctt tgcaatctat cttctcgaaa tcgagactgt tttctattct
     2881 atatgagagt aatacaggat actctatccg tgcagctttt gtcttttctg ctgcaaagag
     2941 ctgtacataa tccaacccgg cactctttga aacatcattg attacattga tgctctcatt
     3001 tacaaatatt cctactttca taattgaaga tggaaccctt gaagttattt tcgcagccgt
     3061 cgatgcgtca atgtaccgtt ttgaaccagg ataaaaaata agccctattg catcaaccct
     3121 tagtgaaact gcaaaatcaa catcttcctt tctggtaaaa ccgcaatatt tacaaaacac
     3181 ttgaaagctc cttaacaaaa catgatgggt ctcccgattt cataagcgcg cttccaataa
     3241 ggaaaacagt tacgccagta tcggttgaaa tctttttaac atcatcactg gtatttattc
     3301 cgcttgctga tatacgtaaa actgatttag gaacctgctt taaaactttt gatcctgcat
     3361 tacagtctat aaccagtgta ttaagatcac gcatattgac catcacaatt tgtggattta
     3421 ttgccataac cctatcgagc tcttctattc tattgatctc gacaactggt actaccgaac
     3481 gttccattgc gtattcataa agcgaggtga gctcataatc agttaaactc tgagcgatta
     3541 aaagcaccat atcggcacca taacgatagg caagctctat ctgctctttg aagtagataa
     3601 attctttaca aagaacaggt acggtaaccg cattacagac atcacgcaaa tcctcaaaac
     3661 tgccagaaaa aaaattagaa tcggttaaca cgcttattgc tgccgttcca ccattttggt
     3721 aagcaattgc ctgtgaagca gcttttgcag aggtatcaag agtttgtgtt ggtgagcttc
     3781 ttttgacctc ggcaataata tcaattgcat ctgaaggtat aaatggatat gcaggtctaa
     3841 accgactgtc actataatta agtaactgct tcacattgtc ttgctcagaa ttttttattt
     3901 cgcgcatttt gtttaactta aattttgtca tttgtctaac cacgaagcct ttttatcgaa
     3961 tgcataacat tgccactgat tatctgttca taagccattt tgaatccctc ttctattgaa
     4021 gaggctttat caagaacgca aagagcaaga gcagaattta atgaaagcaa cttaacaaga
     4081 ggagtatctt tcttttcact aacagtttct aaaaacagct gagcattctg ttgcgcgcta
     4141 taatgtgaag gtattgactt agcctcctct tttgtaatat aaggcgcagg gtcaatttca
     4201 tactctctaa tattatgtcc ttttatctcg gccacttgtg ttattgcgaa tggcgaaact
     4261 tcatccatcc catcttgcga cgaatagaga actagatgat caaaacctat ttgcacagct
     4321 gcctgagaat agactggaag atatgcttta tcaaatatac caatcatttg tcttttaggt
     4381 ctggttggat tggtaagtgg cccaagcatg ttaaaaagtg tccgggttgc gagttgcttg
     4441 cgtaccggaa ccgcatattt cattgccgga tggtagttag gcgcaaacat gaaaataaaa
     4501 tttgttttat caaaatatgt ccgagcttct tcggctttaa gatttatcgg aatgccaagt
     4561 gcctcgtaaa aatctgctga tcctgataat gaactgacag agcggttgcc atgtttgaca
     4621 ataggacagc ccatagagtt tagtataatt gctacagcgg atgacacgtt aaacgaatgt
     4681 ataccatcac cacccgttcc acaggtatca aaaagattgt caccagtaat accaggcgaa
     4741 agtgcaaatt cacgcatcga aacagcagca cccgctatct catccgcgct ttccttacgc
     4801 attttcattg cgattaaaag agcagcaagc tcacactctg caacttcacc ctggaaaagc
     4861 gaattaaaaa gtgtagttgt ttcaatttta ttcaaaaccc caccgtcata gactttttta
     4921 agcatctctt ttgtattcat ggcatatctc cgtaaaattt tttattattt tcataccgtg
     4981 ttcacttaaa tagctctccg gatgaaactg aaccccgtag atcggcaacg tattatgttc
     5041 aatagccata atttcgttat ccgaacttag agccgtaatt tttaatgttg aaggcaattc
     5101 atccgatgag accgcaagtg aatgatatcg caccacttta aaactatcag gtatatcagc
     5161 gaataacctg ccaccagaat gtttaacaga atcaatttta ccatgcattg tttttgctgc
     5221 atgcacaacc ttaccaccaa agacaagacc aacagcctgc attcccaggc aaataccaag
     5281 cactggtaaa tatgtaatag ttttcaatac atcaagggtt attcccgaat tatcaggtgt
     5341 cgatggtcct ggagaaagaa gaatcccatt ataacgtgaa aaatctatca gtgaaaagtc
     5401 acaatcatta gaaaatatct gaacatcttc accacatttt ttaatatagt taacaacgtt
     5461 atatgtgaac gagtcatagt tatcaattac gcatatcatg tttttcctca cactttacgg
     5521 ggcattgcat aagcaagaga agatgccaac gcgcccagtt tatttcttat ttcctgatac
     5581 tcattttccg gcttactatc ctgaacaata cctgcgccag cctgtaaata gttaccttct
     5641 tttgtaaaat atgctgttcg tatggtaatg caagtatcaa gatattgatt aaaacccata
     5701 taaccgatag cccctgaata tatgtcacga ggttgcgttt cacattcgtc aataatttct
     5761 atagccctta cctttggagc cccgctaact gttcctgcag gaaaggattc tttcaaaaca
     5821 tcgatcatat gtttatcttc atcaagaacc gactcaacat ccgaaaccag atgaataacg
     5881 tgtgaataat tttcgggttt cataaaccga gtaactttta cactttcagg tatcgcaatc
     5941 cgagataggt cgttacgcgc aagatcgaca agcataagat gttcagcaca ctctttttca
     6001 tcagccagca tggcttcagt ggcaagttta tctgcttttt catcacctgt acgttttgct
     6061 gtaccggcta ttggccttaa cattgcttca cgcccgctac agcgcacatg cacttctggg
     6121 gaacttccta caattactat gccgttaact tttataaagt acatgtacgg tgagggattt
     6181 gtttttcgca gacgcaaata gaatctgtaa ggatcaatat cctcaggtat catggccctc
     6241 atagagagaa caatttgcaa agcctcacct tcgtcaatca ttgaaacagc tttcgctaca
     6301 gttgacataa agttagtctt gctctctgta aaacgaggtt caacctcttc agctcttgaa
     6361 ggcatatatg gcagtgttgt ttctgaaatc ttgtttatga tctcttcttc aagttcttca
     6421 aggcgagcaa gtgcattgta gtatgacttc tgcacaccat cctccggata aacacaaacc
     6481 gaaagataat atttttttgt ctcgttatcg aaacatataa attcatcgat atgcataagg
     6541 tatatctgag gaattttatt attctctcgt acaggcttgc gaaggatacc acagtaatta
     6601 accatttcgt atccgaaata tccggcaaga cccccgttaa aatgaccgaa ctgctcaaag
     6661 gcaggtgatg tataacgatc aatctctttt ttaaggtaac caaaaacctt gtcctccggg
     6721 aaagagccag tatgaacatt gtctttgtac atctgtccgc tctcgaaccg gtaatggtga
     6781 cgaggattaa tacccataaa agaaaatctg gtattatcac tgttgtcacc aaggctctcg
     6841 agaaacatta cgtcacccgc ttcagacatt acttttagaa ttaatagatg cccgggctcc
     6901 tcaaggttta gttcccgata aaccggaatc cggctatact gcttggcaag tttttgatac
     6961 tcgttaaaat caggataatg cataagtcct cccctggagt ttaaaaaacc gcctataggc
     7021 ggtctgttgt ttaaatacaa aaaaaccgcg aagcccgcgg tatcataaca gtcaaagaca
     7081 aatgtatacc acaggcaaaa ctatatttta ccgcgccacc accagttctt aatatttata
     7141 cttttattaa acattctcgt aatattcctt ttcaatcttg ttcttgttgt ttctatgtat
     7201 agaagcacca aggtgtttct atacatagaa ataccttgca gttattgtca acttttttat
     7261 ggattttttt taaaaaaaca tggcgagaaa agaaaagctg gataagaaac cggagaaacg
     7321 aagaacagga gaacaggaaa ataccggagg aaatgggaaa actaggatag tgagaaatcg
     7381 ggaaaacggg gacggtgacg ttttcccgag aaacggggac ggtgacgctt tcccgagaaa
     7441 cggggacggt gactttttcc c
//
LOCUS       DAOTGZ010000137         7453 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_3064
            NODE_11889_length_7453_cov_4.532036, whole genome shotgun sequence.
ACCESSION   DAOTGZ010000137 DAOTGZ010000000
VERSION     DAOTGZ010000137.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868088
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 7453)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7453)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:22:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,785
            CDSs (total)                      :: 2,751
            Genes (coding)                    :: 2,732
            CDSs (with protein)               :: 2,732
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 12 of 19
            Pseudo Genes (internal stop)      :: 5 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7453
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11889_length_7453_cov_4.532036"
                     /isolate="AMR_MDS_3064"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1246)
                     /locus_tag="P5123_09290"
     CDS             complement(<1..1246)
                     /locus_tag="P5123_09290"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HRX16497.1"
                     /translation="MNRNKKTLLIILLWITIVGVGGSFSAFIILKHNLQLKAANLDLL
                     FNQIISDKDDSSIKREASDIAFKTGLIIYIKVENFIWQSRDEYDYPKMIEGNREKTWF
                     HIRSQKAGYYLKKGKHSALFIVDLFRIRTAIMFIIALIFLCAAPIVLFYVLLKRKNSN
                     VHNSDLLLKGNEMQSPLLQKENYYVEASSQSEQDVISREQTENDYLRSRDALKINNEI
                     SEQKFNQDYSQINRLLSDLVRVKDHLLIDVNQKLKAPLSRIKLAADFLDGDASLEIQK
                     DVKLMQKIIEDVFKTFRTAYTSDALKIENIDLLYFFNGLRQKQRIDRSMLLLKIDQEK
                     TIKADRRLFTILIGNLLDNAFYHAKDSGQPVTVLCETDNGITAITIRNHGPAIPVDDI
                     PYIFDPFYQVKNVSKTEETKTGL"
     gene            1553..2662
                     /locus_tag="P5123_09295"
     CDS             1553..2662
                     /locus_tag="P5123_09295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006421755.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HRX16498.1"
                     /translation="MDRYLEQIIIDDLKKKLVILVGPRQVGKTWLAKKIMFSYKNPSY
                     LNYDNLKDKNIILNQSWLPSTDLLIFDELHKMPQWKNYLKGVYDTRPDSLHILLTGSA
                     RLDTFRHQGDSLLGRFFIHHLLPFCPSEIMDMPLEKFIERGGFPEPVLAESSTDAKRW
                     RNQYMEGIIRDDILDFENVHNFKTLKTLLEMLRQRVGSPLSFKSIAEDLQVAPNTVKK
                     YIGIFESLFIIFRITPYSKNIARSLLKEPKIYFFDTGLVKGDDGLRFENLTAVSLLKH
                     CYTSRDVFGENRTLHYLRTKDGREVDFCIVDDDKISYIVEAKLQDDQPSKSLHYFKER
                     YQLKAVQLTAFLRNEYSKDGIEIVKGERFLRELYS"
     gene            2973..4454
                     /locus_tag="P5123_09300"
     CDS             2973..4454
                     /locus_tag="P5123_09300"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRX16499.1"
                     /translation="MKIISFILVATLFLTCCSSFESETSGKTNKDFPDADGVTSIHVT
                     TTTVLNDLDSATLGININYLTDKNLGLNSRRSINSSLADLNIRILRFPGGEKSDNYLW
                     AQHPFTNANHSLAIKSETAWPNSDDRFCSDPVTGRLTEETLNFDDIIKVASGINAKLM
                     VVVAYDSIFFKGVGKPLKEDLIESAVEWVKYANIDNNFGVNLWMIGNETWNSDSYNGS
                     ATALQYSADLAEYSTAMKAVDPTIKIIANSKGYDYTRVLLENAKEHIDYIGLSNYPIH
                     EWSGGYSEYKKYSGSLLGETDEVLRAIQSYGTAQKIIITEFCAIDWSGEWTSTNDTGH
                     ALCSFEIAGEQLNNPNIEHSFFWTTQWVNNDNHPYHAFDQNGNKTAAGKSLWLWNRFP
                     LTQMVSIESTTLIRCFASYDSLSKKLYLYMINKDNEYNRKISIVLNDFSTKSIGKSYT
                     MKGQSSDDSNPSLTEFLGTAISDSGLNITLPALSITVIELNGE"
     gene            complement(4535..6244)
                     /locus_tag="P5123_09305"
     CDS             complement(4535..6244)
                     /locus_tag="P5123_09305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013036623.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-binding protein"
                     /protein_id="HRX16500.1"
                     /translation="MTGAKILVQSLKDLGVTHVFGYTGAAILPVIDEIGQSDIEFVVN
                     SNEQSAAFSAGGYSRSGSKMGVAVVTSGPAITNTLTAVSDANADSIPLLVIAGQVPEY
                     KLGTDSFQHINVSQIFEGAAKAVVLVDDIFRIESIIKDSWFLAQNGKPGPVVIDIPLN
                     LQKAEGAYQDLPVDLFKSAYQSEAHLSEQQCFSFFELLASSKRPLLYVGGGVNSVAAS
                     ELVRKFSKRFNIPSVNTLMGKGAIDERDDTSLGLLGMFGTPYANKIIEENDLFIALGV
                     RWDDRVAEKVGFAIKSRIAYIDINPAKVKQIRIERQPDFSAVGDVSHILGDLLGYACT
                     KEIDLDFSGWGNHAAKIKRRWPLNYNRESEKIQQANVIDTINRLLPSDTVITTGVGNH
                     QLLAAQYIKMSTPRSFLTSGSFGTMGFGMPAAIGAYYANPGKNVLVIDGDGSFRMNMG
                     EVHTIGQYGLPVKIVLLNNRSDGMVRNLENLSFEGRHSATERQIDADFSKMASVFGFE
                     YSRRISKADEISSALNELFSATGPALLEVITDIDEAIYPLVPVGKGYHEMVLGPYIKE
                     IAD"
     gene            complement(6679..7050)
                     /locus_tag="P5123_09310"
     CDS             complement(6679..7050)
                     /locus_tag="P5123_09310"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF025054.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HRX16501.1"
                     /translation="MYGYFVNDRLCAVQVINTVQEDEYLQVGWTYTDNNPLVLHRLCV
                     SPEYQNRGIARKMILFAENYAEENNYRTIRFDAFMENPISVNIYRKMGYNVAGTVRFR
                     KGDFFCFEKRQFNLTRHKNII"
BASE COUNT         2343 a         1474 c         1321 g         2315 t
ORIGIN      
        1 ccaaaccggt tttagtctct tcggttttag aaacattttt gacctgataa aacggatcaa
       61 agatgtaggg gatatcatca acaggtattg ccgggccatg gtttcgaatt gtaatcgcag
      121 tgatgccatt atcagtttcg cataaaaccg taacgggctg cccggaatct ttagcatgat
      181 aaaaggcatt atccagaaga ttcccgataa gtattgtaaa gagtctgcga tctgctttta
      241 tagttttttc ttgatcaatt tttaacaaca gcatcgacct gtctatccgt tgtttttgcc
      301 gcagaccgtt aaaaaaatag agaaggtcaa tattttcaat ttttaaggca tctgaggtat
      361 aagccgttct gaaagtttta aatacatcct cgattatttt ttgcattagt tttacatctt
      421 tttgtatctc caaacttgcg tctccatcaa gaaaatctgc tgcaagtttt atacgtgata
      481 gaggagcttt tagtttttgg ttaacatcta tcaaaagatg atctttgacc ctgacaagat
      541 ctgaaagaag gcggtttatt tgtgaataat cctgattaaa tttttgctcg gatatttcat
      601 tatttatttt caaagcatct ctgcttcgaa gataatcatt ttctgtttgt tctctagaga
      661 ttacatcctg ctcggattgt gaagatgcct caacataata gttctctttt tgcaataaag
      721 gcgactgcat ctcatttcct tttaataaca gatcactatt atgtacgtta gaatttttcc
      781 gttttaatag aacataaaac aaaacaattg gagcagcgca aagaaaaatc agagcaataa
      841 taaacataat ggctgttcga atacgaaata gatctacaat aaaaagagcg gaatgtttcc
      901 cctttttaag ataataccct gccttttgag accgaatatg aaaccatgtt ttctctctat
      961 taccttcaat catctttgga taatcatatt catcacgaga ctgccagatg aaattttcta
     1021 ctttaatata tataataagt ccggttttaa atgcaatatc tgaagcttct cttttaattg
     1081 aagaatcatc tttatcagat attatttggt taaatagaag atccagattt gctgctttta
     1141 actgtagatt atgtttaagt ataataaatg ccgaaaatga tccacctacg cccactattg
     1201 tgatccaaag cagtattatt aatagagttt tcttgtttct gttcattttt atccttgaaa
     1261 gacgacttgt aatcagattt ttcactattg taataattac ccgataccta tcaggcaatt
     1321 attacaaaaa aataatcaga tattaaatta cacatgcttt accggttctg tcaactgaga
     1381 actgctcgga catgattagg atatataatt tctattaaaa ttgacacagt taattttaca
     1441 ataaattgtt tatttgagct ctattaggca atcatcttct acttgttgac tattttgcaa
     1501 gctattgcta aaatgtcttg actattttgc aatgcaatgc taaatagtcg ttatggacag
     1561 atatcttgag cagataatta ttgatgactt aaaaaagaag ctggtcattc ttgtagggcc
     1621 acgtcaggta ggaaagacat ggctggctaa aaaaatcatg tttagctaca aaaatccctc
     1681 atatctaaac tatgataatc taaaagataa aaacattata ctaaaccaga gctggctacc
     1741 ctctaccgat ttactaatat ttgatgaact tcataagatg ccccagtgga aaaactattt
     1801 aaaaggtgtt tatgatacaa gacctgattc acttcatata ctgttaacag gaagcgcgcg
     1861 tcttgacaca ttccgccatc aaggagattc acttttagga cggtttttta tccatcacct
     1921 gctccccttt tgcccatcag agatcatgga catgccgctc gaaaaattta tagaacgagg
     1981 tggctttcca gagcctgttt tagcggagtc atcaacagac gcaaaacggt ggcgaaacca
     2041 gtatatggaa ggtataatca gagacgatat actcgatttt gaaaacgtgc acaactttaa
     2101 gacattaaaa acacttcttg aaatgttacg ccaacgcgta ggaagccctc tttcttttaa
     2161 atcaattgct gaagaccttc aggtagcccc gaatacagta aaaaaatata tcgggatatt
     2221 tgagtcgctt tttataatat tcagaataac tccttactca aaaaatattg cgcgctcact
     2281 tcttaaggaa cctaaaatat atttttttga tacgggtctt gtaaaggggg atgacggttt
     2341 acgctttgaa aatctgacag cagtgtctct gctaaaacac tgttatacat caagagatgt
     2401 ctttggagaa aaccgtacgt tgcactattt acgtactaag gatgggcgag aagttgattt
     2461 ttgcatagtg gatgatgaca aaatatctta tattgtagaa gcaaagctac aggacgatca
     2521 gccatccaaa tcacttcact actttaaaga acgataccag ttaaaagcag ttcagctaac
     2581 tgctttttta agaaatgaat attctaaaga cggtattgag atagttaaag gtgaaaggtt
     2641 tttacgcgaa ctctatagct gagttgtatt gaatatactg atacagaata aattagcaac
     2701 tttgctctat tttttatatt ctatatacag actggatatt atcattgcaa tataaatgct
     2761 acttgtaaaa tttaactaag tttttggcaa actcaagcaa taatatattc aacaagatag
     2821 cgtatgtaaa cagcaagagt catcttacaa atgagcataa gaacttactg aaaagtctta
     2881 tagaatagat acttaaacga tgttactctt tggagcatcg taattattcg aagttcactt
     2941 aataacaaca attttttgat tatggagtta caatgaaaat tatttcattt attttagtcg
     3001 caacattgtt tctaacttgt tgttcatctt ttgaaagtga aacttctggc aaaacaaata
     3061 aagatttccc tgacgcagac ggggtaacat ctatacatgt tacgaccacg accgtgctta
     3121 atgatcttga ttctgctaca cttggaataa atatcaacta tctcactgat aaaaatctgg
     3181 gtcttaattc aaggcgaagc atcaatagct cacttgctga tcttaatata cgtatcctca
     3241 gatttcctgg gggtgaaaag tcagacaatt atctttgggc gcaacatccc tttacaaacg
     3301 caaatcactc tctggcaatc aaatctgaaa ctgcttggcc aaacagcgac gaccgatttt
     3361 gcagtgatcc cgttactgga agattaactg aagaaactct taattttgat gatattataa
     3421 aagtagcatc tggtattaat gcaaaattaa tggtggttgt agcttatgac tcgatcttct
     3481 ttaagggagt tggtaaacct ttgaaagaag acttaattga atcagcagtc gaatgggtaa
     3541 aatatgctaa tatcgataat aatttcggag ttaatctttg gatgattggc aacgagacat
     3601 ggaatagcga ctcatataac ggtagcgcaa ctgcgttgca gtattcggct gatctcgctg
     3661 agtattcaac agcaatgaaa gcagttgacc cgacaattaa aattatagct aattctaagg
     3721 gatatgacta tactcgagtg ctgctcgaaa acgccaaaga gcatatcgac tatattggac
     3781 taagtaacta cccaattcac gaatggtcgg gaggatactc tgaatacaaa aaatattccg
     3841 gttcacttct gggcgaaact gatgaagttt tacgtgctat acaatcttat ggtacagcac
     3901 aaaaaataat tatcacagag ttttgtgcta ttgactggtc cggagagtgg acttcaacaa
     3961 atgacacagg ccacgcttta tgttcttttg aaattgcggg agaacaattg aataatccga
     4021 atatagagca ctcatttttt tggacaacac agtgggtaaa taatgataac catccatatc
     4081 acgcgtttga ccaaaacggc aacaagactg ctgctggaaa atcactctgg ctctggaatc
     4141 gctttcctct gacgcagatg gtttctatag aatcaacaac tctgataaga tgctttgctt
     4201 catatgactc gttgtcaaaa aagctatatc tatatatgat aaacaaagat aatgaataca
     4261 ataggaaaat ctcgatagta ttgaatgatt tttctacaaa atcaattgga aagagttata
     4321 ctatgaaagg gcaatcttca gatgatagta atccatcatt aacagaattt ttgggaactg
     4381 caatttcaga ttcagggctt aatattacac tacccgctct ttcgataaca gttatcgaat
     4441 tgaacgggga gtaactattt caaaccaatc atctaaacac ataaacttgt ttttgatatg
     4501 gcgtacaaac gtacaccata tcattaatat aagcttaatc tgcaatttcc ttgatataag
     4561 gccctaacac catctcatga taacctttgc cgacggggac aagcggataa attgcttcat
     4621 caatatctgt tatgacttca agaagagcag gtccagttgc ggaaaacaac tcattcaagg
     4681 cactgctgat ttcatctgct ttactgattc gtctgctata ctcgaaacca aaaaccgaag
     4741 ccatctttga aaaatccgcg tctatctgcc tttcagtcgc cgaatggcgt ccttcaaagg
     4801 agaggttctc cagattacgg accattccgt cactgcggtt attcagcaga actatcttta
     4861 ccggtaatcc atactggcct attgtgtgaa cctcgcccat gttcatcctg aagcttccgt
     4921 ctccgtcgat aacaagaaca tttttaccgg gatttgcata ataggcgcca atagctgccg
     4981 gcataccaaa gcccatcgtt ccaaagctgc ccgaagttaa aaaactgcgt ggagtcgaca
     5041 tttttatata ctgtgcggca agcagctgat gattaccaac cccggttgtt attacagtat
     5101 ccgaaggtaa aagccggtta atagtatcta ttacattggc ctgctgaatt ttttcgcttt
     5161 cacggttata gttcaagggc catcttctct ttatttttgc ggcatgattt ccccaacctg
     5221 aaaaatccag atctatctcc ttagtacagg cataaccgag aagatctcca agtatatgtg
     5281 acacgtcacc aacagccgaa aaatcgggct ggcgttctat tcttatctgt ttaaccttgg
     5341 ccggatttat atcaatgtat gcaattctgg acttaatcgc aaaacctact ttttcagcaa
     5401 cgcggtcatc ccagcgaacg ccaagggcta tgaaaagatc gttctcttca attattttat
     5461 ttgcataggg agtgccaaac atgcccagaa gccctaaaga tgtatcgtct cgctcatcaa
     5521 ttgccccctt gcccatcaga gtattaacag aaggaatatt aaagcgctta ctgaatttgc
     5581 gtacaagctc agacgctgca acactattga ctccgccgcc tacatacagc aatggtcttt
     5641 ttgaagatgc cagaagttca aaaaaagaaa aacactgctg ctctgataaa tgtgcttcac
     5701 tctgatacgc gctcttgaaa aggtctactg gaagatcctg ataggctcct tcggcctttt
     5761 gaagatttaa tggtatatca attacaaccg gtcccggttt tccgttttga gccagaaacc
     5821 aggaatcctt gataattgat tcaatacgga aaatgtcatc gacaaggaca accgccttgg
     5881 cagcgccttc aaatatctgg ctgacattga tatgctgaaa agagtcagta ccaagtttgt
     5941 actcaggcac ctgccccgca attaccagaa gcggaatact gtcggcattt gcatcggaaa
     6001 ctgctgttag agtatttgta attgcgggtc ccgaggtcac taccgctacc cccattttgc
     6061 tgcccgaacg tgaatacccg cccgcgctga aagcagcaga ctgctcgtta gaatttacta
     6121 caaactcgat atctgattga ccgatctcgt ctataaccgg aaggattgcg gctccggtat
     6181 aaccgaagac atgagtaacg cccagatcct ttaaagactg aaccagaatt tttgcacctg
     6241 tcatgattgt ctcctatatt ttaatcatat tttattttct atattgtact ctcatctttg
     6301 cctctgtttt aacaactccg gaatctgtta tcacatctgc cattgcaggc atatcataca
     6361 gattctgcct gtttttcgat gtagagagtt cacaaaaaaa aagcccctcc cggttaatcc
     6421 ggaaggggct ttgctgtttt ctttacatca gagatcccta cggtaataac cgtaaaactt
     6481 ttttatgcag gacgctgacg acgactatag aaacagtcga acaatgcaaa gaaatgtctg
     6541 ccaaaaagtt tttcaccggg atcttctccg aatttttgat tattaattaa acaaagaata
     6601 ttatgatatc tgaatcggtc aataaaaata cataattttt ttttaaaaaa aataacttga
     6661 ggtagttagc aaaactgatc atattatatt tttatgtctg gtcaaattga actgtctttt
     6721 ttcaaaacag aaaaaatcac cctttctgaa tctaacggta cccgcaacat tgtaccccat
     6781 cttgcgatag atatttacag atatgggatt ctccataaat gcatcaaacc tgatcgtcct
     6841 gtaattattc tcttcggcat agttttctgc aaagagaatc atcttcctgg cgatacctct
     6901 gttttgatat tctggcgaca cacacaatct gtgaagcaca agaggattat tatcggtata
     6961 ggtccatccg acctgcaaat actcatcctc ctgcactgta ttgattacct gaaccgcaca
     7021 gagtctatcg tttacaaaat aaccgtacat ggatgaagca ttgataggaa catcgaataa
     7081 ttactttttt tagaatagct acgtcaccaa aagcataata taataaggat aatttttctc
     7141 gcaagctcaa aaaaccgcgg taaaaaggca ctttaaggat agtgccattt taccgcttta
     7201 taattgcata atcccctgct gatccatctg agctgttgcg gatctgaata tatcaataac
     7261 atcatccagt tgatcaattg taaccttttt tatgattccg attacctgtc atataagcat
     7321 aatgaatttc attcaggata tcacgctttc tgtttagcag gattcttttg atcggcacca
     7381 ttgccattaa tcggcaacgg catcttctgg ttgtaatccc ttttattgat attttctttg
     7441 attctttcaa tcc
//
LOCUS       DAOTGZ010000138         7400 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_3064
            NODE_11987_length_7400_cov_4.760517, whole genome shotgun sequence.
ACCESSION   DAOTGZ010000138 DAOTGZ010000000
VERSION     DAOTGZ010000138.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868088
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 7400)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7400)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:22:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,785
            CDSs (total)                      :: 2,751
            Genes (coding)                    :: 2,732
            CDSs (with protein)               :: 2,732
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 12 of 19
            Pseudo Genes (internal stop)      :: 5 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7400
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11987_length_7400_cov_4.760517"
                     /isolate="AMR_MDS_3064"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..382
                     /locus_tag="P5123_09315"
     CDS             <1..382
                     /locus_tag="P5123_09315"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="MinD/ParA family protein"
                     /protein_id="HRX16502.1"
                     /translation="ITDAYGIIKSIASQSTEKTIRLIVNRCQSVSEARRVADRIINIA
                     SQFLNIKVENLGFIFEDSFVAKAVRHQKPFVSSYPKSRAAGCVNIIADKIAGREPEGS
                     YGGGISGLFKRLFTQVDDEEDDIE"
     gene            554..1057
                     /locus_tag="P5123_09320"
     CDS             554..1057
                     /locus_tag="P5123_09320"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRX16503.1"
                     /translation="MFILKKIEYKVSAVLAVFALVLSPLVGIISGINAGVVVLRTALM
                     AIFFAGIGFVVIFVLKRFVPEVYSVLNRNQNPLDMDVESIDAGISGEQEYEVYNPEDE
                     TDEDAETVPDSNHGNFDPGLAPSTSESLGIKDRAMGRHILEERGVKYEPKIMAEAIRT
                     MMSKDEK"
     gene            1072..1872
                     /gene="whiG"
                     /locus_tag="P5123_09325"
     CDS             1072..1872
                     /gene="whiG"
                     /locus_tag="P5123_09325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014803734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor WhiG"
                     /protein_id="HRX16504.1"
                     /translation="MNKKKIAEYEAINEDDLWQDFAKSNNQEIRDYFVIKYAPLVKYV
                     AGKVAMGMPQNIEFDDLVSYGIFGLMDAISKYNPDRGIKFKTYAMTRIRGSIFDELRS
                     IDWIPRSIRQKAKEIENIIAELENKLGRTVEDEEISKELGISIEEFHSILNKVSGTSM
                     VSLNDIWYLGDDNDELSIMETLEAPDNMNPDVLVEKEEIKEHIVEAIKKLPDKEKKVI
                     VLYYYEDLTLKEIGQVLDVTESRISQLHTKAIMRLRGRLGRIKSNLVG"
     gene            1874..3838
                     /locus_tag="P5123_09330"
     CDS             1874..3838
                     /locus_tag="P5123_09330"
                     /inference="COORDINATES: protein motif:HMM:NF015894.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FapA family protein"
                     /protein_id="HRX16505.1"
                     /translation="MSNLKSLLKNIATESPSSENELEVYADSIRQGLDLAAKELGIDI
                     SMLDYEVAQRGNSGLFGFGRVPYKLYVRALSEQEAFADLNDLDQKLSKNISDELSREF
                     TDPNAPGTFKVRVTKSGIWFTVKPPKGKGRKVSIADVAQKMDHLQIKDFNQDLVKKSV
                     AAQSGKPVRIGEWVPNIELDGTMYIEISDDEMYAMAHFEPPRFSGRHMELDDVLHSLK
                     NAGVVVGIDEEAINKYLDEMNYSSPLVAAAGVKPKNGTDAYVEYKVRVEKEISFDNDD
                     DSVDFKDLNLIENVVVGQLLAIKRPATNGVEGRTVKNNIIPVRPGKDIAIKHGEGTIL
                     SDDGMELTAERNGQVILKGDKICVDEVLLVQGDVNNVTGNIVMMGSVVITGNVLDEFS
                     VKASGNVEVRGSVQKAVIEAEGDIVIRQGINGRDEGRIETTSGSIYAKFVNRANLIAE
                     KNVVVTEELLQSRVDAGGTVFCNGRRAQIVGGVIRAGDEVNAKQIGSDSYTKTFIYVG
                     MNPKVLQQMNDLSASLDSTAEELEKLELDIKTLTSRKKRSRLSEEQEEKLQLLTDRQA
                     KLTKRHDDIAGEREELEQYLDMLEHKGIVCAEKQLYPGVEIIIKNERLPVQDPYSNVS
                     ISMKDKSWVFGNYIKPDSVDTMTSSSRRRR"
     gene            3839..4165
                     /locus_tag="P5123_09335"
     CDS             3839..4165
                     /locus_tag="P5123_09335"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRX16506.1"
                     /translation="MAVSPIDLQTNMGHMHDVAKQAQSRAENLLNQHQFLDRESELES
                     NRHKDRVDANRESDESKMESAKERESREKSKKKHDQTLKSSKKDDQDSDKPHDPLLGN
                     NIDFTR"
     gene            4174..4788
                     /locus_tag="P5123_09340"
     CDS             4174..4788
                     /locus_tag="P5123_09340"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRX16507.1"
                     /translation="MLNFFMILLTNIFTGVIIYLVLTLKLEKSSTGYHIQKYKREIDE
                     MMREFNNSAETNITLLENRIKLCKKMLKLSGSIESLDIRSIEEDAGRQKIHKQITPVK
                     EEEYPIIHDFNIEQKNKNSGSRSFVIDEEIRIEPGRVEDEIPDIEKQLKNGGNQHETF
                     VSLYRQGYSPLKISEASNIALPEVLLILQLNGIEPEAVGDINGF"
     gene            4778..5647
                     /locus_tag="P5123_09345"
     CDS             4778..5647
                     /locus_tag="P5123_09345"
                     /inference="COORDINATES: protein motif:HMM:NF013867.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008983 - protein-glutamate
                     O-methyltransferase activity [Evidence IEA];
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein-glutamate O-methyltransferase CheR"
                     /protein_id="HRX16508.1"
                     /translation="MDFDLIRRLTPEEFRKFADLIYSESGIFMKDSKITLLSNRLRRR
                     LQSLELETFAEYYEYINSQKDRSREIEELLDVVSTNETYFFRNERHFEALINNCLPEI
                     AAKKKEKVLRIWSAGCSTGEEPYTLAICVNECLSLFPGWRIEIIASDIAHSVLDFAKK
                     GCYSGRRIEKVSKTRLERYFDKVPDADGVFKVKSNVSKLVAFSRINLFKDPFPSNIDI
                     IFCRNVMIYFDNLHQRVLVGNFFRSINKDTGFFFIGHSETLHAISEDFSYVKIKDAPV
                     YVPTRRLSEYKEN"
     gene            5628..6128
                     /locus_tag="P5123_09350"
     CDS             5628..6128
                     /locus_tag="P5123_09350"
                     /inference="COORDINATES: protein motif:HMM:NF015908.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis protein CheD"
                     /protein_id="HRX16509.1"
                     /translation="MNIKKIDVGIGEYAVDRSPVIFRTVLGSCVSLNLYDPLILMGGM
                     AHIVLPQFFKSSGKEDRFADKVIPKMIKDLESGGARRDRLVARMAGGANMYSKPGGPV
                     LFDVGNSNIKAVKKIVTDYDIPILSNICGGQVARIVTFDLSTGRVRVDEIKPEVSFMK
                     GDKWKV"
     gene            6116..6982
                     /locus_tag="P5123_09355"
     CDS             6116..6982
                     /locus_tag="P5123_09355"
                     /inference="COORDINATES: protein motif:HMM:TIGR00255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YicC family protein"
                     /protein_id="HRX16510.1"
                     /translation="MESMTGYASVEGKTEQFSFSIEVKTLNTKFLEIYINLPRIIRQE
                     ENVYSSIVKEYINRGKVEVNFDLFDWNEERQVSVNTALIKAYYDQLLTVENKIMGDRK
                     FNIDAILGFDGVVAKGRTSINPSSFEKLTAAYHKALKNAVKMRREEGKSIEKDILLSL
                     KNIGVNVQKITKLTKNSALEQYQKLKERVSGLVGEGADTQRLYTEFALLSDRLDINEE
                     LSRLKDHMSKFKNTCSEKGPIGKKLDFIAQEMFREINTIASKSNRSDVAHCVVDVKNL
                     IDKIREQCRNVV"
     gene            6979..7230
                     /locus_tag="P5123_09360"
     CDS             6979..7230
                     /locus_tag="P5123_09360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003869230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF370 domain-containing protein"
                     /protein_id="HRX16511.1"
                     /translation="MKSQLINIGFGNAVALNRVVAIVSPSSASGKRIREEARDTRMLI
                     DATHGRKTRSIVIMDSNHIILSGMQPETLINRMNQGKEE"
     gene            7232..>7400
                     /locus_tag="P5123_09365"
     CDS             7232..>7400
                     /locus_tag="P5123_09365"
                     /inference="COORDINATES: protein motif:HMM:NF012832.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="guanylate kinase"
                     /protein_id="HRX16512.1"
                     /translation="MTVIISAPSGAGKSTIIRELLNVDSSLEFAISSTTRPRRETEQH
                     AREYYFVDKDTF"
BASE COUNT         2411 a         1145 c         1679 g         2165 t
ORIGIN      
        1 cattacagat gcctatggta tcataaaatc aatagcatcg caatcaacag aaaagactat
       61 taggttgatt gttaatagat gtcaatctgt ttctgaggct cgtcgtgttg cagacagaat
      121 aataaacatt gctagtcagt ttttaaatat taaagtcgaa aatcttggat ttatttttga
      181 ggacagcttt gttgcaaagg cggtaagaca tcaaaagcct tttgtgtcca gctatcccaa
      241 atcgcgcgcg gcggggtgcg taaatattat tgccgataaa attgctgggc gtgaaccaga
      301 gggatcttac ggtggaggca tctctggtct ttttaagcga ctttttactc aagttgatga
      361 tgaagaggat gatattgagt aatatttcat tctgattgtt tcttaacatc tgtttttttt
      421 gtatattatg agttttactg ttttttttat gtaatttgct ctcttatggt tatccttttg
      481 atgttgatgt aaaaaaaggg ttgatttttt ttgtattgtc caaaattatt tttaaaggtg
      541 acataactat cttatgttta ttttaaaaaa aattgaatat aaggtttcag ctgttctggc
      601 agtgtttgcc ctagtgttgt cacctctagt gggaattatt tccggaataa atgccggtgt
      661 tgttgttttg cgaacagcat taatggctat tttctttgcc ggtatcggtt ttgttgttat
      721 ttttgttctt aagcggtttg ttcctgaagt ttactcggtt ctcaaccgca atcagaatcc
      781 ccttgatatg gatgttgaat ccattgacgc agggatttct ggtgaacagg aatatgaagt
      841 ttacaacccg gaagatgaaa ctgacgaaga tgccgaaaca gtgccggata gcaaccacgg
      901 aaattttgat cctggtttgg caccatcaac gtctgaaagt ctcggtatca aggatcgtgc
      961 tatggggcgc catattcttg aagaaagagg ggttaagtat gaacccaaga ttatggcaga
     1021 agctattcgt acgatgatga gtaaagacga aaaataaccg tggatggtat gatgaataag
     1081 aagaaaattg cagagtatga agcgataaat gaggatgatc tttggcaaga ttttgctaag
     1141 agcaataatc aggagattcg tgattacttt gttattaaat acgcacctct tgtaaagtat
     1201 gttgctggaa aggttgcgat gggaatgccc cagaacattg aatttgacga cttggtttcg
     1261 tacggtattt ttggtcttat ggatgcgatt agtaaatata atccagatcg tggtataaaa
     1321 tttaagactt atgcgatgac acgtattcgc gggtcaatat ttgacgaatt gcgctctatt
     1381 gactggattc cgcgttctat acgacagaaa gccaaagaga tcgagaatat tattgccgaa
     1441 cttgaaaaca agttggggcg tacggtcgaa gatgaggaaa tatctaaaga gttgggtatc
     1501 tcaattgaag agttccattc catactaaat aaagtcagcg ggacttcaat ggtttctctt
     1561 aacgacatat ggtatcttgg tgatgataat gacgagcttt ccatcatgga gactcttgaa
     1621 gcaccggata atatgaatcc ggatgttctt gtcgaaaaag aagaaattaa agagcatata
     1681 gttgaagcta taaagaaact acccgataaa gaaaaaaaag tgatagttct ttactattat
     1741 gaagacttga cactaaaaga gattggtcag gttcttgatg taacagagag tcgtatatca
     1801 caacttcaca ccaaggcaat catgcgtttg cgcggccgtt taggacgtat aaaatcaaat
     1861 ctggtcggtt aatatgtcca atttgaaaag ccttctcaaa aatattgcca cagagagccc
     1921 ttcttctgaa aacgaactcg aagtttacgc cgattctatc cgacaggggt tagatcttgc
     1981 ggcaaaagag ctgggtattg atatatcaat gctcgattat gaggttgctc agaggggaaa
     2041 ctcaggccta ttcggttttg gacgtgttcc atataaatta tacgttcgcg cgttaagtga
     2101 gcaagaggcg tttgccgatc taaacgatct tgatcaaaag ctatccaaaa acatatcaga
     2161 tgaattatct cgtgaattta ctgacccaaa tgccccaggt acatttaagg tgcgagttac
     2221 aaaaagtggt atatggttta ccgttaaacc acctaaaggt aaaggccgta aagtttcgat
     2281 tgccgatgtt gctcaaaaga tggatcatct gcaaataaaa gattttaatc aggatttggt
     2341 caaaaaaagt gttgcggctc agagtgggaa accggtgcga ataggagagt gggttccaaa
     2401 catagaactt gatggaacga tgtatatcga aatcagtgat gatgagatgt atgccatggc
     2461 tcattttgaa ccgccacgtt tttccggacg acatatggag cttgatgatg ttttacattc
     2521 tctcaaaaac gcgggagttg ttgtcggtat tgatgaagaa gctattaata aatatctaga
     2581 cgaaatgaat tattcttcac ctcttgtggc tgcggccgga gttaaaccaa aaaacggaac
     2641 cgatgcctat gtcgagtata aagtcagagt cgagaaagag atatcttttg ataacgatga
     2701 cgattctgta gattttaaag atctgaattt aattgaaaat gttgtagttg gacagctttt
     2761 ggcaattaag cggccggcta caaatggtgt tgaagggcga acggttaaaa ataatattat
     2821 tcctgttagg ccaggaaagg atattgctat caaacatggt gaaggaacca tactctcgga
     2881 tgacggcatg gagcttacag ctgagagaaa tggtcaggta atattgaaag gtgataagat
     2941 atgcgttgac gaagtacttc ttgtacaggg agatgtaaat aatgttaccg gcaacattgt
     3001 catgatgggg tctgttgtta ttacaggaaa tgtactggac gaattttcag taaaagcttc
     3061 aggaaatgtg gaagtgcgtg gttctgtaca gaaagctgtg attgaagcag agggcgatat
     3121 tgtaattcgt cagggaataa atggtcgtga tgaaggaaga atcgaaacta ccagcggttc
     3181 aatttacgct aaatttgtga acagggcaaa tctgatcgcg gaaaaaaatg tagttgtaac
     3241 ggaagagctt ttgcaatcac gagtggatgc ggggggaact gtattttgca acggaagacg
     3301 ggcacagata gtaggtggag tgattcgggc cggtgatgaa gttaatgcaa aacagatcgg
     3361 atcagattct tacacgaaaa cttttattta cgtgggtatg aatccaaagg ttttacagca
     3421 aatgaacgat ctttctgcat cacttgattc aaccgcggaa gaacttgaaa aacttgagct
     3481 tgatatcaag acgcttacat cacgtaaaaa aagatcaaga ttgtcagaag agcaagaaga
     3541 aaaactgcaa cttttaactg ataggcaggc caaacttacg aaacgtcatg atgatatagc
     3601 tggtgaaagg gaagagcttg aacagtatct tgatatgctt gaacataaag gcattgtatg
     3661 tgccgaaaag cagctctatc caggagtaga gataatcata aagaatgaac ggttgcctgt
     3721 tcaagatccc tattcaaatg tcagtatatc aatgaaagat aaaagttggg tttttggaaa
     3781 ttatattaaa cccgattctg tagataccat gacatcctct tcgcgaagga ggcgatagat
     3841 ggcagtatca cctatagatc ttcagacaaa catggggcat atgcatgatg tagccaaaca
     3901 agcacaaagt cgagccgaga atttgcttaa tcagcatcaa tttttagatc gagaatcaga
     3961 actggaatcg aatcggcata aggatcgtgt ggatgcaaat cgtgagtccg atgaaagcaa
     4021 gatggaaagt gcgaaagagc gtgaaagtcg tgaaaaaagt aaaaagaaac atgatcagac
     4081 attaaaaagt tcaaaaaaag atgatcaaga cagtgataag ccacatgatc cccttctggg
     4141 aaataatata gattttactc gataggacct gatatgctta atttctttat gatattgctt
     4201 actaatattt ttactggtgt aattatttat ctcgtgttaa cattaaaact tgaaaaatct
     4261 tcaaccggtt atcatattca gaaatataaa cgggaaattg acgagatgat gcgtgagttt
     4321 aataatagcg ccgagacaaa cattacactt ctcgaaaaca gaataaagtt atgtaaaaaa
     4381 atgttaaagc taagcggatc aattgaaagt cttgatatta gatcaattga agaggatgct
     4441 ggtaggcaaa agatacataa acaaataact cccgttaaag aggaagagta tccaattata
     4501 catgatttta atatagaaca aaaaaataaa aacagcggat cgcgttcatt tgtaattgat
     4561 gaggagatta gaattgaacc gggacgagtg gaagatgaaa tccctgatat agaaaaacaa
     4621 cttaaaaatg gtggtaatca gcatgagaca tttgtttcgc tttaccgtca ggggtattcg
     4681 ccattaaaga tatctgaagc ctcaaatata gctctacctg aagtactttt aattttgcag
     4741 cttaacggta ttgagccgga agccgttgga gatattaatg gattttgatc tgataagacg
     4801 tctcactcct gaagagttca ggaagtttgc tgatcttata tattctgaaa gcggaatatt
     4861 catgaaagat tctaaaatta ccctgctttc taaccggttg cgaagacgcc ttcaaagttt
     4921 ggagcttgag acttttgcag agtattatga atatattaat agtcagaaag atcgatcacg
     4981 tgaaatagaa gagttactcg atgttgtatc aactaacgaa acctatttct ttcgtaatga
     5041 acgccatttt gaggctctta tcaataattg tttgccggag atcgcagcaa aaaagaaaga
     5101 aaaagtttta agaatatgga gtgctggatg ttcaaccggt gaggaaccat atacattagc
     5161 catttgtgtt aatgaatgtc tttcgctttt cccgggatgg cgtatagaga taatcgcttc
     5221 ggatattgct cattctgtgc tcgattttgc gaaaaaagga tgttattccg gtcgcagaat
     5281 tgagaaggtg agtaaaactc gacttgaaag atattttgac aaggttccag atgcggacgg
     5341 tgtatttaag gtcaagagta atgtcagcaa gcttgttgct tttagcagaa taaatctgtt
     5401 taaagatcct ttcccgtcta acattgatat aatattttgc cgaaatgtta tgatctattt
     5461 tgataatctt catcaaaggg tgctggttgg taattttttc cgatctatta acaaagatac
     5521 aggttttttc tttatcggcc actccgaaac tctgcatgca atatctgaag acttctctta
     5581 tgtaaagatt aaggatgcgc ctgtttatgt cccaacccga cggctcagtg aatataaaga
     5641 aaattgacgt cggtattggc gagtatgctg ttgacagatc accggtaata ttcagaactg
     5701 ttctaggttc atgcgtgtca ttgaacctgt atgaccctct aattcttatg ggagggatgg
     5761 ctcatattgt tttgccacag ttttttaagt ctagcggaaa agaggatagg tttgccgaca
     5821 aggtgatacc aaaaatgatc aaagatttgg aaagcggtgg tgccagaaga gataggcttg
     5881 tggcaagaat ggctggtggt gctaatatgt acagtaaacc aggtggtccg gtgctttttg
     5941 atgttggcaa ttcaaacatt aaagccgtta aaaaaatagt aacagattat gatataccca
     6001 tattgtcaaa tatatgcggt ggacaagtgg caaggatagt gacattcgat ttatcaaccg
     6061 gaagagtccg agttgatgaa attaaaccag aagtgtcgtt tatgaaaggt gacaaatgga
     6121 aagtatgaca ggatatgctt cagttgaagg gaaaacagag cagttctctt tctcgataga
     6181 ggttaaaact ttaaatacaa aatttctgga aatatatatt aatttaccta gaattattcg
     6241 ccaggaagag aatgtttatt cttcaattgt taaggagtac ataaacaggg gaaaggtaga
     6301 ggtaaatttt gatctttttg actggaatga agagcggcag gtttcggtaa atactgccct
     6361 tatcaaggca tattacgatc aacttttaac agtagaaaat aaaataatgg gtgatcgaaa
     6421 gtttaatatt gatgcaatac tgggttttga tggtgttgta gcaaaaggaa gaacgtcaat
     6481 aaatccctct tcatttgaga aactaacagc agcttaccat aaagctctta aaaatgctgt
     6541 caagatgcgc cgagaagagg gtaaatccat tgaaaaagat atactcttgt ctcttaaaaa
     6601 tataggtgtt aatgttcaaa aaattacaaa acttacaaaa aatagcgctt tagagcaata
     6661 tcaaaaatta aaagagcgag tatcggggct tgttggagag ggtgctgata cgcaaagatt
     6721 gtatacggaa tttgctctat tatcggatcg gcttgacata aacgaggagt tatcacggtt
     6781 aaaggatcat atgtctaagt ttaaaaatac atgttctgaa aaaggtccga taggtaaaaa
     6841 acttgatttt attgctcagg agatgtttcg tgaaataaat accatcgctt caaaatcaaa
     6901 tcgttcagat gtagcgcatt gcgtagttga tgttaagaat ttaattgata aaattcgtga
     6961 acagtgcagg aatgtggtat gaaaagtcag ctgataaata tagggtttgg taatgcggtc
     7021 gctcttaatc gtgtagttgc tattgtttca ccgtcatcgg catcgggcaa gcgaatcaga
     7081 gaagaggcgc gggatactcg gatgctgatt gatgctacac atggtagaaa aactcgttct
     7141 attgtgatta tggacagtaa tcatattatt ctttctggta tgcaaccaga aacattgata
     7201 aatcgtatga atcaaggtaa ggaagaatag tatgacagtt ataatttctg ctccatctgg
     7261 tgctggtaaa tccacaataa tcagagagct tcttaatgta gatagctcgc ttgaatttgc
     7321 tatatcttca acaacccgac caaggcgaga aactgagcaa catgctcgtg aatactattt
     7381 cgtggataag gacacattca
//
LOCUS       DAOTGZ010000139         7357 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_3064
            NODE_12066_length_7357_cov_4.021501, whole genome shotgun sequence.
ACCESSION   DAOTGZ010000139 DAOTGZ010000000
VERSION     DAOTGZ010000139.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868088
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 7357)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7357)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:22:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,785
            CDSs (total)                      :: 2,751
            Genes (coding)                    :: 2,732
            CDSs (with protein)               :: 2,732
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 12 of 19
            Pseudo Genes (internal stop)      :: 5 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7357
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12066_length_7357_cov_4.021501"
                     /isolate="AMR_MDS_3064"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(3..494)
                     /locus_tag="P5123_09370"
     CDS             complement(3..494)
                     /locus_tag="P5123_09370"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HRX16513.1"
                     /translation="MNINVEEYYVKYGPMVYRRCLAMLKNEDRAADAMQDVFVKILRY
                     KGSLEHKYPSSLLYTIATNICLNIIKADKRVSPAEVEDVLHTIACYNEGESKVIVDEY
                     LDSIFEDEKEGTREIAVMFYIDRMKLQQISDVTGLSVSGIRKRLSKVKQIAASFREDY
                     HEQ"
     gene            complement(531..1163)
                     /locus_tag="P5123_09375"
     CDS             complement(531..1163)
                     /locus_tag="P5123_09375"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRX16514.1"
                     /translation="MKKTIFLLIIFASLPLFCEKLLSDQQNIESSTPRQMETQSLLGD
                     RNYRLTGFGGVCNTVNSDMSFFGARGALVVNERFVLGVQSMNLVHPHDASDIEGVDPE
                     QNEGEIDIRYGGALLGAHFFQKKLFNLSVTSIIGGGSLDITRDQDSDFNSSVFFLIEP
                     TVIGYVNLTRWARAGAGLSYKYTKGIDIQGITDDSFRKMSMVLTIDFGKF"
     gene            complement(1274..2224)
                     /locus_tag="P5123_09380"
     CDS             complement(1274..2224)
                     /locus_tag="P5123_09380"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRX16515.1"
                     /translation="MYQYYRFIFFQFIIVISFLMGNYLRERVKNRDVLTRFLIKSNLF
                     VLEPFLAFWTGWNIRFSSDLLFLPTAGLFVAVMTFAAGMFLSRYTGQSSIRRETFTIS
                     SSLSNQGYTMGAFICYILLGEQGLALALLLIIYFFFFTYGFIFTYASFKGSGKVEKVT
                     LAVLFKKFFTLNNMPMYALIAALLLNRLGINRPEIEINPDIFIFPVVIFSFIMLGLSY
                     NLKSLSFFSRSSMFMFLLKFIAAPFLGIVFTILFNVDPLYTKIIIAESMMPVAVYSVV
                     TANLFKLDSEYAASLFVINTVHFLIISFPLFYFLSRFSFI"
     gene            complement(2337..4100)
                     /locus_tag="P5123_09385"
     CDS             complement(2337..4100)
                     /locus_tag="P5123_09385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014625102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-dependent [FeFe] hydrogenase, group A6"
                     /protein_id="HRX16516.1"
                     /translation="METVNVKFNGKPYEVQKGLTILRAAEAAQIKIPKLCDHPDLEPW
                     AACGLCIVKLENSPKMFRSCAMDVQEGMSIITHDSEIVKVRKTVLELILSRHPNDCLK
                     CGRNGNCELQNLAADFGIREVPYPQMVPDLPKDTSTSSIVLDPSKCVLCGRCAQVCQN
                     MQNVWALEFLGRGFETRIAPAADVDLADSPCVKCGQCSAHCPVGAIYEKDQVAEVWNK
                     INNQCPGCQDIYPVVQIAPAVRVAIGEAFGYEPGEISTGKLYAALRRLGFKAVFDTNF
                     SADLTILEEGTEFVKRFTSNEKIPLITSCCPAWIDYLEKYDHDMIEHFSTAKSPQEMM
                     GAMIKTYFAEQQKVDASKIYSVSVMPCTAKKFECDRNEDMFSSGYQDVDVVLTTRELA
                     RMIKQAGIDFAKLEDEHADEILAEYTGAGTIFGATGGVMEAALRSAYFLITKEELPDV
                     NLVPVRGLDGVKEATIDIKGTSVNVAVAHGLVNVEYVLDKIRDALAKGEKSPYDFVEV
                     MACRGGCVGGGGQPYGATDEIRAKRAAGIYKDDEQSEKRCSHQNPMITKIYKDFLGEP
                     GSHKAHKLLHTSYKPRKLYNK"
     gene            complement(4117..5919)
                     /locus_tag="P5123_09390"
     CDS             complement(4117..5919)
                     /locus_tag="P5123_09390"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014625103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoF"
                     /protein_id="HRX16517.1"
                     /translation="MAYRSYILVCGGTGCESSHADKIYQNLKNEIEANQLFAQVQVIK
                     TGCFGFCEQGPIVKILPENTFYVQVKPEDAKELVEEHIVKGRPVQRLIYNPNEDKNRS
                     SLVTEEKDIQFYQKQFRVVLRNCGMIDPEVIEEYIARDGYAALEKVIFDMKPDDVIEE
                     LKKSGLRGRGGAGFPTWMKWNFTKNATNSETKYVVCNADEGDPGAYMDRSTLEGDPHS
                     ILEAMTIAGFTVGAQKGFIYIRAEYPLAINRLKIAIKQAKEMGLLGENILGSDFSFDI
                     EIRLGAGAFVCGEETALLASIEGKRGMPIPRPPFPATHGLWKKPTVINNVETYANIPM
                     VILKGGDWFSSIGTESSKGTKVFALTGKIKNSGLIEVPMGITLREIIYDIGGGIPGGR
                     KFKAAQSGGPSGGVITEEFLDTPIDYENLAKLGSIMGSGGLIVMDEDDCMLDIAKFYL
                     DFTVDESCGKCAPCRIGGKQLYGILDGISKGNGEIEDIQKLKDISLAMQRASLCGLGQ
                     TAPNPVASTLRYFENEYMEHIVDKKCSAGVCKDLLQFVIHKDKCVGCTVCARKCPVNA
                     ISGERKQPHEIDQSACIKCGACYEACKFQAITRG"
     gene            complement(5934..6329)
                     /locus_tag="P5123_09395"
     CDS             complement(5934..6329)
                     /locus_tag="P5123_09395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013970149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(2Fe-2S) ferredoxin domain-containing protein"
                     /protein_id="HRX16518.1"
                     /translation="MARLTLEELRKLRENKKAEIQKRETDNKDIDVIVGMGTCGIAAG
                     AKEALDAFIAEIDNHNLKNVRIKQTGCMGFCTYEPTVEVKVPDMPDTIYNKVSADVAK
                     HIVEKHLVQKQLVSEYVFDRPSVDILKNK"
     gene            complement(6346..6885)
                     /locus_tag="P5123_09400"
     CDS             complement(6346..6885)
                     /locus_tag="P5123_09400"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HRX16519.1"
                     /translation="MQYSVCDYLLDLTQNSVESGATFITLDILESQDKLEICIGDDGC
                     GMDGQTLQKAKDPFFTDGVKHTNRKVGLGIPFVMQAVESTGGEFDIKSEKGVGTSLYL
                     SFIKAHVDMPPIGDLAGTLRSIMLFDAQYDLLIYRSNNEESYRVSRSELKDAVGEFSD
                     TDSLVLIKQFFSSQEEDIS"
     gene            complement(6895..7338)
                     /locus_tag="P5123_09405"
     CDS             complement(6895..7338)
                     /locus_tag="P5123_09405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018525849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase subunit E"
                     /protein_id="HRX16520.1"
                     /translation="MNQFISEWKEKPGNLIMILHRVQEEYGYIPREVALELSRMLDVP
                     LAKIYGVITFYHFFKLTKPGKNIIQVCMGTACYLKGGADLISELERLLGLPVNGTTDD
                     GEFSLEAVRCIGCCGLAPVMTVNGEVFGKITAEQLPGVISKFKGN"
BASE COUNT         2291 a         1704 c         1234 g         2128 t
ORIGIN      
        1 aatcattgtt catgataatc ctccctgaaa gatgcagcaa tctgttttac cttggaaaga
       61 cgctttctga tgcccgatac agataacccc gtaacatcag atatctgctg caacttcatc
      121 ctatctatat aaaacataac agcaatctcc cgtgtgccct ctttttcatc ttcaaatata
      181 gaatcaagat attcatccac aatcaccttt gattcacctt cgttgtaaca tgcaatcgtg
      241 tgcaacacat cttcgacttc cgctggactg acccgcttgt ctgccttgat aatatttaaa
      301 caaatattgg ttgctatggt atataacaga cttgaaggat atttatgttc cagacttccc
      361 ttgtaccgta aaatttttac aaaaacatcc tgcatggcat ctgctgccct gtcctcgttc
      421 tttagcattg caaggcatct tcgatagacc attggcccat acttaacata atactcttct
      481 acattgatat tcattcagta acccgtttaa gggtacttag taccctttct ttagaacttt
      541 ccaaagtcaa tggttaaaac cattgacatt tttctaaaag agtcgtcagt tattccctga
      601 atgtcaatac cttttgtata tttatacgat agacccgctc ctgctcgcgc ccatcttgtt
      661 agattaacat acccaatcac tgtgggttca ataagaaaga atacactgct attgaaatct
      721 gaatcctgat ctcttgtaat gtctaaagag cccccgccaa ttattgatgt aacagataag
      781 ttgaagagtt ttttttgaaa aaaatgtgct ccaagtaagg ctccaccata tcgaatatct
      841 atttcacctt cattttgttc cggatcgact ccctcaatgt cacttgcatc atgaggatga
      901 acaagattca tactttgaac gcccaagaca aatctttcat taacaaccaa agctcctctt
      961 gccccgaaaa agctcatatc tgaattaaca gtattgcata ctcctccaaa accggttagc
     1021 ctataatttc tatcaccaag cagtgattga gtctccattt gtcttggtgt actactttca
     1081 atattttgct gatcagataa aagtttttca caaaacagag gcaatgaagc aaatatgatt
     1141 aaaagaaaga ttgtcttttt catatgattc ctctccagtc cattattaca taatcttatt
     1201 gccttctgtt tttatctgta ttatataaca gataaaaaca gaaaaacgtt acatcttaaa
     1261 caaaatttta gcactaaata aatgaaaatc ttgataaaaa atagaacaac ggaaacgaaa
     1321 taataagaaa atgtacggta tttatcacaa aaagagaggc ggcatattcg gaatccagtt
     1381 taaacagatt tgccgtaaca accgaataaa ccgcaaccgg catcatcgac tctgcaatta
     1441 ttatttttgt atatagcgga tcgacattaa acagtattgt aaatactatg ccaagaaatg
     1501 gcgcagcaat aaattttaac aaaaacataa acatggaaga ccgtgaaaaa aaacttaacg
     1561 attttagatt ataactaagc cccagcatta taaaagaaaa tattacaaca gggaaaataa
     1621 atatatctgg gttaatctct atttccggac gattaattcc caaacgattc aacaagagag
     1681 cggcaattaa cgcgtacata ggcatattat taagcgtgaa aaatttttta aaaagcacag
     1741 ccaaagtaac cttttctact tttccactac ccttaaaaga agcgtatgta aaaataaacc
     1801 cataggtaaa aaagaagaag tagatgataa gaagcaacgc cagcgccaaa ccttgttcac
     1861 caagaagaat atagcatata aaagccccca ttgtatagcc ctgattggaa agtgaagacg
     1921 atattgtaaa agtttcacgg cgaatactac tttgaccagt ataacgggat aagaacattc
     1981 cagctgcaaa agtcatcact gctacaaaaa gaccagccgt tggaagaaac agaagatcac
     2041 tgctaaaccg gatattccat ccagtccaga atgccagaaa cggttctaaa acaaacaaat
     2101 ttgatttaat tagaaatctt gttaaaacat cacggttttt cactctttca cgcagatagt
     2161 tacccattaa aaatgatata acaataataa attgaaaaaa tataaaacga tagtactgat
     2221 acatatttac ccctaaagta ctttgtctat agccatctgc atcttcgcca aaaaaaaaga
     2281 gcgcctaata ggcactcttt ttttatattg catctctgta ataaacagat caagaactat
     2341 ttgttataca gtttacgcgg tttgtatgac gtatgcaata atttatgagc cttgtgactt
     2401 ccgggctctc caagaaaatc cttataaatc tttgtaatca ttggattttg atgtgaacat
     2461 cttttttcgg actgttcatc atccttgtaa ataccagcag cacgcttggc acgaatttca
     2521 tctgtagcac cataaggctg accaccgcca ccaacgcaac cgccacgaca cgccataacc
     2581 tctacgaaat cgtatggcga tttctcgccc ttggcaagag cgtctctgat tttatccaga
     2641 acatactcaa cattaacaag gccgtgagca acagcaacgt taacgctggt tcccttgata
     2701 tcaattgtag cctctttaac accgtcaagc ccgcgaaccg gaacaagatt cacatctgga
     2761 agctcttcct tggtaatcag gaagtatgcc gaacggagag cagcctccat aacaccacca
     2821 gttgcgccaa aaatagtacc tgcaccggta tattcagcaa gaatctcatc ggcatgttca
     2881 tcttcaagtt tagcaaaatc gataccagcc tgtttaatca tacgggcaag ctctctggtt
     2941 gtaaggacaa catcaacatc ctgatatccg ctagagaaca tatcctcgtt tctatcacac
     3001 tcgaactttt tcgctgtaca aggcataaca gagaccgaat aaattttaga tgcatcgacc
     3061 ttctgttgct ctgcaaagta ggtctttatc atggctccca tcatctcttg aggagacttg
     3121 gcagtagaaa aatgttcgat catatcatgg tcatacttct caagataatc gatccatgcc
     3181 ggacaacatg atgtaataag aggtattttt tcatttgatg tgaaacgctt aacaaactct
     3241 gtaccttctt caagaattgt aaggtccgca gaaaagtttg tgtcaaatac agctttgaaa
     3301 cccaaacggc gcaatgccgc gtaaagttta ccggtagaaa tctctcctgg ctcgtatcca
     3361 aaagcttcac ctattgccac acgcactgcg ggagctatct gaacaacggg ataaatatcc
     3421 tgacaccctg gacactgatt attgatcttg ttccaaacct ctgcaacctg atctttttca
     3481 taaatagcac caaccgggca atgagcagaa cactgaccac actttacaca agggctgtct
     3541 gcaagatcga catcagcagc gggagcaatt cttgtttcaa agccacgtcc caagaattcc
     3601 agcgcccata cattctgcat attttgacaa acctgcgcgc aacgcccgca aagaacacat
     3661 ttagaaggat ctagtactat tgatgatgtc gaagtgtctt taggtaaatc aggaaccatt
     3721 tgaggatacg gaacctctct gattccaaaa tcggccgcaa gattctgcag ctcacagttg
     3781 ccgtttcgac cgcacttaag acaatcattg gggtgccttg aaagaatcag ctccaaaacg
     3841 gtttttctta cctttacgat ctcggaatcg tgagtaataa tgctcatccc ctcctgaacg
     3901 tccatggcac atgaacgaaa catcttggga gagttttcta gctttactat acacaaacca
     3961 caagcagccc aaggctcaag atcaggatga tcacaaagct taggtatttt tatttgagca
     4021 gcttcagctg ctctaagtat cgtgagccct ttttgaactt cgtagggctt accgttaaat
     4081 tttacgttta cagtttccat ttatttccgc tcctccttat ccgcgtgtta ttgcctgaaa
     4141 tttacatgct tcgtaacatg caccgcattt gatacaggca ctctgatcaa tttcatgcgg
     4201 ttgtttacgt tcaccactaa tggcgttaac cggacatttt ctggcgcaga ccgtacatcc
     4261 gacacattta tctttatgga taacaaactg tagcagatcc ttacatactc cggcggaaca
     4321 ttttttatct acgatatgtt ccatatattc gttctcgaaa taccggaggg tagaggcaac
     4381 cggatttggt gctgtctgtc caagaccaca aagagaagct ctctgcatgg caagagatat
     4441 atctttaagt ttctggatat cctcaatttc accgttacct tttgaaattc cgtcaagaat
     4501 tccataaagc tgcttaccac cgattcgaca tggggcacat tttccgcaag actcgtccac
     4561 agtgaaatca agatagaact tcgctatatc gagcatacaa tcgtcttcat ccattacgat
     4621 aagaccacct gatcccatga tagaaccaag ctttgcaagg ttttcataat cgattggagt
     4681 atctaaaaac tcttcggtaa taactccacc tgaaggtcca ccggactgag cggccttaaa
     4741 tttacgacct cctggtatac caccaccaat atcatagatg atttctcgaa gagtaatacc
     4801 cataggtact tcaataagac ccgagttttt gatttttcct gtaagagcaa aaacctttgt
     4861 tccctttgaa gattctgtac caattgaaga gaaccagtcg ccacccttaa gaattaccat
     4921 aggaatatta gcataggttt ctacgttatt tataacagtt ggctttttcc acaaaccatg
     4981 tgtagcagga aaaggagggc gcggtatagg catacccctt ttaccttcga tagaagcaag
     5041 aagagcagtc tcttcaccac agacaaaagc tccagcacca agtcgaatct cgatgtcaaa
     5101 actgaagtcg cttcccagaa tattttcacc taacagaccc atctctttgg cctgcttaat
     5161 ggcaattttt aaacggttaa tcgcaagcgg atactcggca cggatataaa tgaagccttt
     5221 ttgtgcgcca acagtaaaac ctgcgatagt catagcttca agtattgaat gcggatcacc
     5281 ttcgagagtc gatcgatcca tgtaagcacc cggatcaccc tcatcggcat tacaaacaac
     5341 atattttgtt tctgagtttg ttgcattttt tgtgaagttc cacttcatcc aggtcgggaa
     5401 acctgcaccg ccacgtccgc gcaaaccgga tttttttaac tcttctatta catcatccgg
     5461 tttcatatca aatattactt tttcaagagc ggcatatcca tcgcgagcaa tatactcctc
     5521 tataacttca gggtctatca taccacagtt gcgcaaaacg acacggaact gtttctgata
     5581 gaattgaatg tctttctctt cagttaccaa agaagaccgg tttttatcct cattaggatt
     5641 atagatcaga cgctgtactg gacgtccttt cacaatatgc tcttctacga gctccttagc
     5701 gtcttccggt ttaacctgaa cataaaaagt gttttccgga agaatcttaa ctatcggacc
     5761 ctgttcacaa aaaccgaaac agccggtttt gataacctga acctgagcaa acaactgatt
     5821 tgcttcgatc tcatttttta aattttgata aatcttatct gcatgactgg actcacatcc
     5881 ggtaccgcca cagacaagaa tataacttct atatgccatt tgtatccccc ggattactta
     5941 ttttttaaaa tatcaactga tggtctatca aaaacgtact cggaaacaag ctgtttttga
     6001 actagatgct tctctacaat atgtttggct acatcagcac ttactttatt gtaaattgtg
     6061 tctggcatat ccggaacctt tacctcgact gtgggctcat aagtacaaaa tcccatgcac
     6121 ccagtctgtt tgattcggac atttttcaaa ttatgattat ctatttcagc gataaaagcg
     6181 tctagagcct ctttcgcacc tgctgcgata ccgcatgttc ccataccgac aataacatcg
     6241 atatccttgt tgtcagtttc gcgtttttga atctctgcct ttttattttc tctaagcttt
     6301 ctaagctctt caagtgttaa cctggccata ttgtcctccg tcttttcagg atatatcctc
     6361 ttcttgtgaa gagaaaaact gttttattag tacaagcgag tcggtgtcag aaaattcacc
     6421 aaccgcatct ttaagttctg accgcgatac tctgtaagac tcttcattat ttgaacggta
     6481 aattaaaaga tcatattgcg catcaaacaa cattattgaa cgcaaagtac ctgccaaatc
     6541 acctattgga ggcatatcca catgagcttt gataaaagaa agataaagtg aagtaccaac
     6601 acccttctct gatttaatat caaattcacc accagtagac tctactgcct gcatcacaaa
     6661 aggaattccc aggccaacct ttctgtttgt atgtttaaca ccgtcagtaa aaaaaggatc
     6721 ttttgctttt tgaagtgtct ggccgtccat gccacaaccg tcatcaccga tacaaatttc
     6781 aagcttatct tgcgactcca atatatccag agtaataaac gttgccccgg attctacaga
     6841 attctgagtt agatccagaa gataatcaca aacagaatat tgcacaaaga cctcttaatt
     6901 acctttaaac tttgaaatca cacctggaag ctgctccgca gtgattttac caaaaacctc
     6961 accatttaca gtcattacag gagcaagtcc gcaacaacca atacaacgaa ccgcttccaa
     7021 tgaaaattca ccgtcatcgg tagtaccgtt aacaggtaat cccaacaatc tctcaagctc
     7081 actaataagg tcagctccac ctttaaggta gcatgctgtt cccatacaga cctgaataat
     7141 gtttttacct ggcttagtaa gtttgaaaaa atgataaaat gttattacac cataaatctt
     7201 ggctagtggc acatcaagca tacgcgataa ttcaagcgcg acctcgcgag gtatatatcc
     7261 atactcctct tgtacgcggt gaagaatcat aataagattg cccggcttct ctttccattc
     7321 gctgataaac tgattcagct ctgaagaaag ctcaaca
//
LOCUS       DAOTGZ010000140         7334 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_3064
            NODE_12115_length_7334_cov_5.388515, whole genome shotgun sequence.
ACCESSION   DAOTGZ010000140 DAOTGZ010000000
VERSION     DAOTGZ010000140.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868088
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 7334)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7334)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:22:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,785
            CDSs (total)                      :: 2,751
            Genes (coding)                    :: 2,732
            CDSs (with protein)               :: 2,732
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 12 of 19
            Pseudo Genes (internal stop)      :: 5 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7334
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12115_length_7334_cov_5.388515"
                     /isolate="AMR_MDS_3064"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(150..1196)
                     /locus_tag="P5123_09410"
     CDS             complement(150..1196)
                     /locus_tag="P5123_09410"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRX16521.1"
                     /translation="MLTCAFLQFRVPLFSQHGLDGTLVSNVWAKLFRNRNELHYNISS
                     AANKKFNLRLNRYQDPLPLMYFDWQGFQICYYDYYAFNGYSGYTDDSSAFVRIKNRDH
                     TLYQLGGKYYFHGFLYDVNEYLDKKKDPYTLFFHDISGDGKPELVLCAGTRGAYSYWT
                     VYIFEIKAGTVRNILTYNCESAGPIDNGNSKNWQKMWNNKTWDLSNNNQKNLAGHFIK
                     DIDNNGVPEIIMLNSGIEQLLGATHGPRALLVIEWNGNRFIDKTRKFPLLARKAALGY
                     LDDSKERKVTSLEAAMPYYANMILAGEEKKAEAWLLRNGSSEIINWLDNKNAMMLLHR
                     ALRPDLSVKTGPSL"
     gene            complement(1368..1775)
                     /locus_tag="P5123_09415"
     CDS             complement(1368..1775)
                     /locus_tag="P5123_09415"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRX16522.1"
                     /translation="MKFSLVIIALALTISCCNSKTEITAKKVRPDYYPSNNSWEKLSL
                     RDAGCDKKLFKIMEKRLSEEKQINAVVILRNGYRVYEYNGYPVFFAWGAREQYIFVVP
                     DLQLVAVFASAYSGTPAMTPPEYMREYVIAGCN"
     gene            2299..3264
                     /locus_tag="P5123_09420"
     CDS             2299..3264
                     /locus_tag="P5123_09420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002600238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="substrate-binding domain-containing protein"
                     /protein_id="HRX16523.1"
                     /translation="MKSLKKLLVLVVAASLLFGLASCKKSSGSSGDLIKVGIINNPPS
                     ESGYRAANVADFEKVFTKENGYDVSTFYSLKNDEQLNAASQFITDGVDYIFLSAAATD
                     GWASVLSRAKEAGIKVFLFDRMLNADPDLYEAAVVSDMTNQGDTAVKWLKAQNLPEYK
                     VIHIQGAMGSDAQIGRTAALDKEFAAGTMKMIVQQTATWDEAEAKKIVESVINSGEKF
                     NVIYAENDGMAKGAVAALDDAGITHGVDKDVVVMGFDCNKWALRELLAGNWNYDGQCS
                     PFQASVIDEMIKTGNIPSKKVISEEKGFDAKTITQKDIDTYGLGD"
     gene            3398..4918
                     /locus_tag="P5123_09425"
     CDS             3398..4918
                     /locus_tag="P5123_09425"
                     /EC_number="7.5.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016322835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter ATP-binding protein"
                     /protein_id="HRX16524.1"
                     /translation="MKNDTVLSMRGISKSFPGVRALHNVDFTLCEGEIHALMGENGAG
                     KSTLVKVLTGVYQKDTGKINIAGTDKELRIKSPQDAQNAGISTVYQEITLCPNLTVAE
                     NIFIGRGNYRFINWRSQEKRAEEILTKLKIPTSATRQLGTCSLAVQQMVAVARAVDME
                     CKVLILDEPTSSLDEQEVALLFSLMRDLKARGVGIIFITHFLEQVYEVCDRITVLRNG
                     ELVGAYDINELPRVELISKMMGKELEEISELQHQKKTVANEDIPLVYEAFGLSSTQGT
                     KPFDFKIKKGEVNGFTGLLGSGRSESVRAIFGADRITGGKIKINEKDTKISRPKDSMK
                     HGIGYLPEDRKGDGIIEDLSVRENIILALQVLKGFFHPFSRAEAEEFADKYIKLLGIK
                     TASMDTPIKSLSGGNQQKVILARWLLTNPQYLILDEPTRGIDIGTKVEIQKLVLKLAN
                     DGVSVTFISSEIEEMLTTCSRLIVMRDRNIVGELKGEQMTQTKIMHTIAGEAAQNA"
     gene            4911..5978
                     /locus_tag="P5123_09430"
     CDS             4911..5978
                     /locus_tag="P5123_09430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002704118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HRX16525.1"
                     /translation="MLKSLNIKKLSHSQLAIPLFALFLVIVFNVFRDISFFSIHVITN
                     NYGNTVLSGNLVSIINGASELVLLAIAMTLVTSATKGQDISVGAAAAIAGSVFVRVLR
                     GNEITMPVIFLALATACIVAILFGLFNGALVAFFDIQPMIASLILFTAGRPIAYWING
                     GATPNVDSPLLSYIGSFIPGIPIPSPILIILVFGILVTLALRFTNLGLYIQSVGINER
                     AARLNGINPVLMKLLAFMILGVCVTIAGAINVCRIGLINHETILIDIEMDAILAVAIG
                     GNALSGGKFKLAGSIIGAYIIQALTITLYAMKVSSTAVKAYKAVVIIAIVVIGSPVVQ
                     QHAVQLRNRLFKKPIKLTENN"
     gene            5980..7044
                     /locus_tag="P5123_09435"
     CDS             5980..7044
                     /locus_tag="P5123_09435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002600235.1"
                     /note="membrane component of a putative sugar ABC
                     transporter system; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease YjfF"
                     /protein_id="HRX16526.1"
                     /translation="MPIRKLKNLFSRDIGNKPKEPISSTALLLTITICAFSAMYIFAM
                     IVWGGGFLNPQQFLDIFNNNAYLIVISCGLTIVMIAAGIDISVGGVVALITMTCVIFL
                     EDYSGSIAGSIFLALGIGLTIGLVQGFLVAYLKIQPFIVTLAGMFFAKGLTTIVSSVP
                     RTVTNNTFLEFKQFRVVVPGIGSCGKTGNFIPARIEPGVIIALIIVLAVWVMLKWTRF
                     GRNLYAVGGSDQSALMLGINVKRTQFLSYVICGLFAGIGGYVYLLHTGAGNSANAYQA
                     EIQAIASSIIGGTLLTGGVGSVIGTLFGVMSLKTFSNIVIASGLREPYWQSITTGLML
                     CFFILLQSVIIAYRKKKGVG"
BASE COUNT         2281 a         1435 c         1477 g         2141 t
ORIGIN      
        1 gataacttcg cggtatttga ttactcaatt ggacaggata taacagacta tcttgttgta
       61 ataaatacag atgcggatgg taaactggat tatatggcaa tggaaagtta aaactcttaa
      121 aaattcaagg gtaatctact gatcacgatt tataaacttg gcccagtctt taccgaaaga
      181 tcaggccgta atgccctatg taataacatc atggcatttt tgttatcaag ccagtttatg
      241 atttcactgc ttccattacg caaaagccag gcttctgctt ttttttcttc accggcaagt
      301 atcatattgg cataataggg catagctgct tcgagagatg tcacttttcg ttcttttgaa
      361 tcatcaaggt atcccagcgc cgcttttctg gcaagaagtg gaaactttcg tgtcttatcg
      421 ataaatcggt ttccattcca ctctattaca agcagagcac gcggaccatg agtagcaccc
      481 agaagctgtt ctatgcccga gttaagcatt attatttcag gcacaccgtt attatcaata
      541 tcctttataa agtgaccggc gagattcttc tgattattgt tacttagatc ccacgtttta
      601 ttgttccaca ttttctgcca atttttgctg tttccattat caattggtcc cgcgctttcg
      661 caattgtacg taagaatgtt tctaactgtt cctgctttta tttcaaaaat ataaactgtc
      721 cagtaagagt atgctcctcg tgttccagca cacaaaacaa gttcaggttt accgtctcca
      781 cttatatcat gaaaaaaaag agtataagga tctttttttt tatcaagata ctcattaaca
      841 tcataaagaa agccgtgaaa ataatattta ccacccaact gataaagagt gtgatctctg
      901 ttttttatgc gcacaaaagc cgacgagtca tccgtataac cagaataacc gttaaacgca
      961 taatagtcat aatagcagat ctgaaaacct tgccagtcaa agtacataag cggaagagga
     1021 tcctgataac ggttaagacg caaattgaat tttttatttg cagcagagct aatattataa
     1081 tggagctcgt ttctgtttct aaagagcttc gcccaaacat tagaaaccag ggtgccgtca
     1141 agcccatgct gtgaaaacag cggcacccta aactgtaaaa aagcacaggt taacaaaaaa
     1201 aacgtccggc atctcactat tgctttaacc tcacataaga ataacatatc gtgaacttct
     1261 aaaaattaca atgtccaaaa gacagcattc tttaagacat attatagtta atttttttta
     1321 gagactacct ataatcataa aacagtttcc ggatgcatag tgcattgcta attgcatcca
     1381 gctataacat attctcgcat atattctggc ggagtcatgg caggagtacc actatacgcc
     1441 gatgcgaaaa ccgcgaccag ttgaagatca ggtacaacaa aaatatactg ttcacgagct
     1501 ccccacgcaa aaaatacagg gtatccgtta tattcatata cccgataacc attacgaagt
     1561 attactaccg cattaatctg cttttcttca gaaagccgtt tttccatgat tttaaataat
     1621 tttttgtcac agcccgcatc ccgtagagat agtttttccc aactattatt tgaaggataa
     1681 taatctggcc gtaccttttt tgcggttatt tctgtttttg aattacaaca ggaaatggtc
     1741 aacgccaaag caattataac taatgaaaac ttcatcctct aagactcctg tactttaact
     1801 gctgttttta ggtattgaat aatataacaa ataaaattct gcacatatat acagaacaat
     1861 ccgggacagt gggccaggat catcaatcta aaaccagttt gttagctatt tataatagga
     1921 aagtaaaaaa gccaatgtgc aaatatttct cacaatgtac aaaatatgtg catttagtat
     1981 acatttaata gtgagaaaca gtcatctcac accaatccag aattaggttt taacttatta
     2041 cagaaagtta caaaagaaag atacactatt tgttgcttat tatggtcaat tttaactgca
     2101 accaaaaaaa aatgcaataa taatcatttc ggcacggtat ctgcatctat aatagaaatt
     2161 attaactcaa ctgctcaatt attaatcact gtgacatact gtaaagcgtt gttatgcaat
     2221 gaaagattat tataaaataa acttcaggca aaacagcgca agtacacaca atgaaaacaa
     2281 attttttgga ggaagtacat gaagagtctt aaaaagttac ttgttctagt tgttgcggca
     2341 tcattgctgt ttggacttgc aagttgtaag aaatcaagcg gaagcagcgg cgatctaatt
     2401 aaagtcggca ttattaacaa tccgccttca gaatcaggtt atcgcgcagc taacgttgca
     2461 gattttgaaa aagtattcac aaaagaaaat ggatacgatg tttcaacttt ttacagcctt
     2521 aaaaacgacg aacagttaaa tgcggcaagt cagtttatca ctgacggtgt tgactacatt
     2581 tttctttctg ctgcggctac tgacggttgg gcttctgttc tttcacgcgc taaagaagcc
     2641 ggaatcaaag tgtttctgtt tgaccgtatg ttaaacgccg atcctgattt gtatgaagct
     2701 gctgttgtat ctgacatgac taatcagggc gacactgctg ttaaatggct taaggcacaa
     2761 aaccttcccg agtacaaagt tattcatatt caaggcgcaa tgggtagtga tgcgcaaata
     2821 ggccgtactg ctgctcttga taaagaattt gccgcaggta caatgaaaat gattgtacag
     2881 caaacagcaa catgggacga ggcagaagct aaaaagattg tagaatcagt tatcaactct
     2941 ggcgaaaaat ttaacgttat ttacgctgaa aatgatggta tggcaaaagg tgctgttgca
     3001 gcgcttgatg acgcgggtat cactcacggt gttgataaag acgttgtagt aatgggcttt
     3061 gactgcaaca agtgggctct tagagaactt cttgcaggca actggaacta tgatggacag
     3121 tgcagtccgt ttcaagcttc tgtaattgac gaaatgatta aaaccggaaa tattccatcc
     3181 aagaaagtta tatccgaaga gaaaggtttt gacgcgaaaa ctataactca aaaagatatt
     3241 gatacatacg gacttggaga ctgatattta actcctatac tcaaacgtta agaacaattt
     3301 agtattacga ctgcgcggcg ccaattgtat gagtttacca cacacttggc gtcgcaattt
     3361 tttaaaattt ccaaagcata aaggcggttt gtaacaaatg aaaaacgata ctgttttatc
     3421 catgcgtgga atatcaaaat cttttccggg tgtacgcgcg ctgcataatg tagactttac
     3481 tctttgcgag ggcgagattc atgcccttat gggtgaaaac ggagccggaa aatccacact
     3541 ggttaaagta ttaaccgggg tataccagaa agacacaggc aagataaaca ttgccggaac
     3601 cgataaagag ttaagaatta aatcacctca ggacgcgcaa aatgccggaa taagcacagt
     3661 ttatcaagaa attacactct gccctaacct gaccgtcgcg gaaaatattt ttattggccg
     3721 tggtaactac cgttttatta attggcggtc acaagaaaaa agggccgagg aaatccttac
     3781 aaagcttaaa atcccgacaa gcgcgacaag acaacttgga acctgttctc ttgctgtaca
     3841 gcaaatggtc gccgtagcac gcgcagttga tatggaatgc aaggttctta ttttagacga
     3901 gccgacatct tccctggacg agcaggaagt ggctctgctt ttttcactta tgcgcgattt
     3961 aaaagcacgc ggagtaggca taatatttat cacacacttt ctagagcagg tttacgaggt
     4021 ttgtgataga ataacagttt tacgcaacgg agaacttgta ggcgcttatg atatcaacga
     4081 attgccacga gtcgagctta tctccaaaat gatgggtaaa gagcttgaag aaatttcaga
     4141 attacaacat cagaagaaaa ctgtagcaaa tgaagatata ccactagttt atgaagcctt
     4201 tggattatcc agtacgcagg gtacaaaacc ctttgacttt aaaataaaga aaggtgaagt
     4261 caacggattt acagggctgc tgggatccgg gcgaagtgag agtgtacgag ccattttcgg
     4321 tgccgaccgc atcactggtg gtaaaataaa aattaacgaa aaagatacta aaatatcgag
     4381 acccaaagat tcgatgaaac atggtatcgg ttacttgccg gaagatagaa aaggcgatgg
     4441 aataattgaa gatctctccg tacgagaaaa cataatactc gcgttgcagg tacttaaggg
     4501 cttttttcat cctttttcca gagcagaggc cgaagagttt gctgacaagt atataaaact
     4561 attaggaatc aaaacagctt ccatggacac gccgataaaa tcgctatctg gcggtaatca
     4621 acagaaagtg atacttgccc gctggctgct gactaatcct caatacctga tactggatga
     4681 acccacacgg ggaatcgata tcggtacaaa agtcgaaata caaaagctgg tacttaaact
     4741 cgcgaatgac ggtgtcagtg tcacctttat ctcttccgag attgaagaga tgctcacaac
     4801 ctgctcgcga cttatagtta tgcgtgatcg taatatagta ggcgaactaa aaggtgaaca
     4861 gatgacacag acaaaaatca tgcacacaat agcaggggag gcggctcaaa atgcttaaga
     4921 gtttaaatat aaaaaaactg tcacattctc aactggcaat accgctgttc gcgctttttc
     4981 tggttatcgt atttaatgtt tttagagaca tcagtttttt ttcaatacac gtaataacca
     5041 ataattacgg aaacactgta ctttcgggaa acctggttag tataataaac ggcgcctcgg
     5101 aacttgtgtt gctggcaata gcaatgactc tggttacttc ggcaacaaag ggacaagata
     5161 taagcgttgg cgcggcagca gcaattgccg gaagtgtttt tgtgcgtgta cttcgtggaa
     5221 acgaaatcac gatgcctgtt atttttctgg cacttgcaac cgcctgcatc gttgcaattc
     5281 tttttgggct gtttaacgga gcactggtcg ccttttttga tattcagccg atgatcgcgt
     5341 cattgattct ttttacggct ggacgtccaa tagcatactg gataaacggc ggagcaacgc
     5401 ctaacgttga cagtcctctt ttaagctata tcggaagttt tatcccgggt atacccatac
     5461 caagccctat tcttattatc ttagtgtttg gaatattggt tacactggct ctaaggttta
     5521 caaatcttgg gctttacatc caatcagtgg gtatcaatga acgggctgca agactaaacg
     5581 gtattaaccc tgttcttatg aaactattgg cgtttatgat ccttggcgtt tgcgttacaa
     5641 ttgccggtgc aattaacgtt tgtcgaattg gtctaattaa ccacgaaaca atactaatcg
     5701 atatcgagat ggatgccatt ctggctgttg caatcggtgg aaacgcgctt agcggtggaa
     5761 agttcaaatt ggccggttca ataatcggag catatatcat tcaggcatta acgattactc
     5821 tatatgccat gaaagtttca tcaacagccg taaaggcata taaagcagtt gttattatcg
     5881 caattgttgt tataggttca ccagttgtgc aacaacatgc cgttcaactt cgcaacaggc
     5941 tgttcaaaaa accgattaaa ctaacggaga ataattgata tgccaatcag aaaactaaaa
     6001 aatctatttt ccagagatat tgggaataag ccaaaagaac caatttcgag cacggcattg
     6061 ctattaacta taaccatttg cgcattttct gcgatgtaca tatttgcaat gattgtatgg
     6121 ggcggtggtt ttctgaatcc ccaacaattt ttggatatat tcaataacaa cgcttaccta
     6181 atcgttattt cttgtggatt aaccatagta atgatagcag caggtattga catttcagtt
     6241 ggtggtgttg tcgccctgat aacaatgacc tgtgttattt ttcttgaaga ctacagcggt
     6301 agtatcgcag gttcaatatt tctggcatta ggcattggtc ttacaatcgg gcttgttcag
     6361 ggttttcttg tcgcgtattt aaagatacaa ccctttattg ttacgcttgc aggcatgttt
     6421 tttgccaaag gcctgacaac catagtcagc agtgttccac gcacggtaac taacaataca
     6481 tttcttgagt ttaagcaatt tcgcgttgta gtccctggta ttggctcttg cggcaaaaca
     6541 ggcaacttta tccctgcccg aattgagcct ggagtaatta tcgctttaat cattgtactt
     6601 gctgtttggg taatgctcaa atggactcgc tttggacgta acctttacgc tgttggtggt
     6661 agcgaccaga gcgcgttgat gcttggtata aacgttaagc gaacacaatt tctatcttat
     6721 gtaatttgcg gactgttcgc tggaataggc ggttatgtat atctgcttca cactggcgcg
     6781 ggtaactcgg caaacgctta tcaggccgaa attcaggcaa tagcttcatc aattataggc
     6841 ggaacccttt tgacaggtgg agttggcagt gtaataggaa ctctttttgg agtaatgtct
     6901 ttaaaaacat ttagtaatat tgttatcgcc tctggactgc gagagcccta ttggcaaagt
     6961 atcacaacag gccttatgct atgtttcttt atacttcttc aaagcgtaat aattgcctat
     7021 cgcaaaaaga aaggtgttgg ttgagactaa aataaccgga tatacaaatt attatttttt
     7081 gacaaacatg aataatgcat aaacgaaaaa atatccatga taagattttt accattatca
     7141 tggatataaa agaatttttc gtgttacccg tgtggctttt atcttgtgct aaaagctttt
     7201 tttataacaa gaacaaccta caagggcaag ctcctttgtc attcgcccag caccactttt
     7261 aaaagaaaat ggtgctggac aacctctgct ggtcgcaaga tgactcttgc tgctcgcaga
     7321 tgcaacctct gctg
//
LOCUS       DAOTGZ010000141         7306 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_3064
            NODE_12180_length_7306_cov_4.464488, whole genome shotgun sequence.
ACCESSION   DAOTGZ010000141 DAOTGZ010000000
VERSION     DAOTGZ010000141.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868088
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 7306)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7306)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:22:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,785
            CDSs (total)                      :: 2,751
            Genes (coding)                    :: 2,732
            CDSs (with protein)               :: 2,732
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 12 of 19
            Pseudo Genes (internal stop)      :: 5 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7306
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12180_length_7306_cov_4.464488"
                     /isolate="AMR_MDS_3064"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(30..2066)
                     /gene="fliD"
                     /locus_tag="P5123_09440"
     CDS             complement(30..2066)
                     /gene="fliD"
                     /locus_tag="P5123_09440"
                     /inference="COORDINATES: protein motif:HMM:NF018852.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009288 - bacterial-type flagellum
                     [Evidence IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar filament capping protein FliD"
                     /protein_id="HRX16527.1"
                     /translation="MPITMGGMASGVDTDSIISKLVEVESQPIRKIESEKNVFRQKKG
                     ALQNLETHLKDIEKSVKDLYGFRASYDDKKAISSRPEIVEAVAGKLARKGTNYITVLE
                     LAGTHKITTDAISKDKKMDAGVIRISVNGEERSLRFRGGTIDKLSEQMNENFEDIVNT
                     NVVNTTSESRVLVLESKVPGKKGEMRISGDLPFLKSVGLSDGYREGEREQVNLVFDST
                     YFYDYAGVSRPFEEDGTLTVAPDGKEVRFSGVLWREYNLATPFEVKKNSILEFDSNFI
                     EPEKEEDEFKALPFKVETGPTESTTIKGITIEGYNVSRERPVHKVDKVVDPEDTIGVG
                     IVYREDEVRKEELFKISKDVKDKQEIPLGERFEGKLIERVIFYSNTGEVAFKDAAFVT
                     PLDETKELKPKNIVSDAANAKLKVNDIELVRESNEGINDVIPGVTLSLLSADPDNPVE
                     ISIDNDIDKAVDKIKKFVEAYNKYLDYNKQLTKAGMTDRKDSYKKVKRENGPFVGDMT
                     IIQLENSLKQTVSSAYPSRAEEPIRLLSQVGLSTGDLNASWETIKEGKLVLDESKLRS
                     AIMENPQGVEMLFGSDTDGDNRIDNGFGYTMENKLKPYIMPGKSIIAAKINLQDESLE
                     RADERIEKLEMHLKNYEEKLRRKFANMEMSISGSKAQSNWMKNQSGGGGGGGGE"
     gene            2391..3446
                     /locus_tag="P5123_09445"
     CDS             2391..3446
                     /locus_tag="P5123_09445"
                     /EC_number="3.4.11.-"
                     /inference="COORDINATES: protein motif:HMM:NF021535.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase"
                     /protein_id="HRX16528.1"
                     /translation="MTTLFKKYKKCTLIVTLLFIVIIAGVYNKKIIVYGFKQGKGQLN
                     ILCNREPIPKFLKRSSLSEEEIYKIELIEEIVNFAVDELGLDDNGAYRTIYDQQNRPL
                     LYLVTAAPEFSLSPYRWDFPIVGSFSYLGFFDLDDAREEAKALEKKGYDVRIREVSAW
                     STLGYFKDPIFTGMLKRSEGSLANLIIHEMTHGTIFVKNNMRFNENIATFIGDTGAIL
                     FLKKRYGNDSHYLKNHINSQQDRDLFYNHIMSETKKLKELYETFDDTMTYEQKKEQKD
                     NMIRSIMQSIADIPFNDPSNYENLKNYRPTNAWFTGYLTYRADLSKIADIYSDNYHSD
                     LRLFVEALKEKKEGILN"
     gene            3614..5194
                     /gene="cimA"
                     /locus_tag="P5123_09450"
     CDS             3614..5194
                     /gene="cimA"
                     /locus_tag="P5123_09450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003871323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043714 - (R)-citramalate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009097 - isoleucine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="citramalate synthase"
                     /protein_id="HRX16529.1"
                     /translation="MNNKKTVTIFDSTLRDGAQAEGISFSVKDKIDILQTLDDFGVTY
                     IEAGNPGSNPKDLEFFETAKKIKLKNSTLAAFGSTRRRSIKVEDDANVQSLLKAETPA
                     VVIFGKSWDFHVTEILKATLDENLEMIKDTIEFFKKKGKEVIFDAEHFFDGYKNNAEY
                     ALKSLQAAIDGGADSLCLCETNGGAYPDEVAEIVNRVVTTFPDSVIGIHTHNDGGMAV
                     ANSVMAVVNGATQVQGTFIGFGERCGNANLSTIIPNLEIKRGYKCLPEGNLQNLTSVS
                     RKIASIANMQLYDFTPFVGNSAFAHKGGMHIDGVNKNSRTFEHIDPALVGNERRFLVS
                     EVAGRSMILNTIQKIDPSIDKNSPETKEIIDILKSMEHNGYQFEGAESSFELVIHRHL
                     KTYKPFFQLEHFKIIGEEPSADKTACTSAIIKVKVDQTSEITAAEGDGPVNALDIALR
                     KALDRFFPELKNVKLTDYKVRVLDTKNATAAKVRVLIESTDGKEIWTTIGVSTDIIEA
                     SWKALVDSIEYKLLNNNS"
     gene            5824..6750
                     /locus_tag="P5123_09455"
     CDS             5824..6750
                     /locus_tag="P5123_09455"
                     /inference="COORDINATES: protein motif:HMM:NF016648.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FecR domain-containing protein"
                     /protein_id="HRX16530.1"
                     /translation="MKNFLITVLILIFTVSCQNSTESEKKSDEPVAKKAVIKIAFSIG
                     EVTIKDKTDTRKAILGEILEEGSTIITGEKSTADLTFFDTGIIHIAENSTLSVSEISP
                     DADGTTGLDMPTGKAALALSKLKNGDLKIKTPTMVAAVRGTAFVIETTKTGSAVSVVQ
                     GAVETGIIDSGTEKSNITVMTNAGEKTTVKTSDISAYTSAKKELKPVKMKRPDIVIAS
                     KELGLIEANRHDLDPETVEYISEEPVKSIEKLLEESEKSLPSPTNDKEESIDTSEIDK
                     AAREAEAKRLKEERRKKRIEAAKESAIAVPAM"
     gene            6771..7139
                     /locus_tag="P5123_09460"
     CDS             6771..7139
                     /locus_tag="P5123_09460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013926160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MmcQ/YjbR family DNA-binding protein"
                     /protein_id="HRX16531.1"
                     /translation="MDIADIRLYCSQKPGSWEDFPFDETTLVIKTGSKMYALISLTNT
                     PVKINLKCDPDRSQLLRRDYPSITEGYHMNKRHWNTVTSSEESSHDVSDKLLKELIDH
                     SYDLVFSSLTKKEKLTITKN"
BASE COUNT         2420 a         1462 c         1298 g         2126 t
ORIGIN      
        1 cctgcaaagc aatataagca aatgccacat tactctccac cacctccgcc accaccacca
       61 gactgatttt tcatccagtt tgactgagcc ttagaacctg agatactcat ctccatattc
      121 gcgaattttc tacggagctt ctcctcataa ttcttaagat gcatttcaag tttttcaatt
      181 ctttcatcag cacgctcaag tgactcatcc tgaagattaa tcttcgcagc aataatactt
      241 ttacccggca taatataagg cttcagctta ttttccatag tgtaaccaaa accgttatca
      301 atccggttgt caccatcagt atccgatcca aaaagcatct ctacaccctg tgggttctcc
      361 ataatagcac ttctcagttt actctcgtca agtacaagtt taccctcttt tattgtctcc
      421 cacgaggcgt tcagatcccc tgttgaaaga cctacctggg ataagagacg tattggttct
      481 tcggcacgag aggggtaagc agaactgaca gtctgcttaa gagagttttc aagctgaata
      541 attgtcatgt caccaacaaa gggaccattt tctcgcttaa ccttcttata actgtctttg
      601 cggtcggtca tgcctgcctt tgtcagctgt ttgttgtaat ccagatattt attatatgct
      661 tcgacaaatt tttttatctt gtcaaccgcc ttatcgatat cattatcaat acttatctct
      721 accggattat ctggatcagc gctcaacaga cttagagtta cacccggaat aacatcatta
      781 ataccttcgt tggattcacg cacaagctca atatcattaa ccttaagttt cgcatttgca
      841 gcatcagaaa caatattttt cggttttaac tcttttgttt cgtcaagagg cgtaacaaat
      901 gctgcatcct taaaagccac ctcgcctgta ttggaataga agataaccct ttctataagt
      961 ttaccttcaa atcgttcacc aaggggaatc tcttgcttat ctttaacatc ttttgatatt
     1021 ttaaaaagtt cctctttgcg aacctcatct tctctgtaaa caatacccac tccaattgta
     1081 tcttccgggt caacaacctt gtccacttta tgtacaggcc gctctcgaga aacattatac
     1141 ccctctattg taatcccctt tatggttgta gattcagtcg gacctgtttc gaccttaaaa
     1201 ggtaaagctt tgaattcatc ttcctctttt tcgggctcta tgaagttgga gtcaaattca
     1261 agaatagagt tcttcttaac ttcaaaaggt gttgcaagat tatattcacg ccacaataca
     1321 ccactgaatc taacctcttt accgtcggga gcaacagtta aagtaccatc ctcttcaaac
     1381 ggacggctta cacccgcgta atcataaaaa taagtcgagt caaaaaccag attaacctgc
     1441 tctctttcac cttctctata accgtcactc aaacctacgc ttttcagaaa aggcaaatct
     1501 ccactaatgc gcatttcgcc ttttttgcca ggaacctttg actctaaaac aaggacacgt
     1561 gactcggagg tggtgttaac aacatttgta tttacaatat cctcgaaatt ctcattcatc
     1621 tgctctgaca gcttgtcaat agtaccacct ctgaaacgca aagatcgctc ctcgccgttt
     1681 acagatatgc gtatcacacc agcatccatt tttttgtctt tgcttatcgc atctgttgtg
     1741 attttatgtg ttccagccaa ttcaagaact gtgatataat ttgtaccctt acgtgccagc
     1801 ttgccggcaa ctgcttctac aatttcagga cgcgaggata tagccttttt atcatcatac
     1861 gaggcacgga aaccgtacag atccttaaca ctcttttcta tatcttttaa atgagtctca
     1921 agattttgca aagccccttt tttctgacga aaaacattct tttcgctctc tatctttcgt
     1981 ataggctgtg actccacctc aacaagcttg gaaattatcg aatcggtgtc aacccctgac
     2041 gccattcctc ccattgttat aggcatttta cacctcccaa aacccccgaa aatatcagaa
     2101 aaacataatt tatgtcaaaa gaacacgagt aacactaccg aacattcaga agatactatt
     2161 ggaacagccc tagagtaact ccttaacaca tattatgtct actagtaccg atatcggcaa
     2221 ttactttatt tccattagta ttttttaaat tttttttcgc cgatttattt tttttacagt
     2281 taatattttg cctttgtcac actatgtcaa aaaacaggat tcacggttta attatgagct
     2341 aaatcgctgt ttaggtatag cggatattat tacaatacgg gattaacaag atgacgacac
     2401 tatttaaaaa atacaaaaaa tgtaccctga tagttacact tctttttata gtgattattg
     2461 caggagtata taataaaaaa ataattgttt acgggtttaa acagggaaag gggcaactta
     2521 atattctttg taatagagaa cccataccca aatttttgaa acgctcaagc ctaagcgaag
     2581 aagaaattta taaaatagaa ctgatagaag agatagtcaa tttcgctgta gacgagcttg
     2641 gtcttgatga taatggagcc tatagaacaa tatatgatca gcaaaacaga cctctgctct
     2701 atctggtcac ggcagcacca gaatttagtc tttcacccta tcgttgggat ttccccattg
     2761 ttgggtcatt cagctatctg ggtttctttg atcttgacga tgcccgcgaa gaagcaaaag
     2821 cattagaaaa aaaaggatat gatgttcgca taagagaagt tagcgcctgg tcaactcttg
     2881 gatatttcaa ggatcccata tttaccggca tgttaaaaag aagtgaaggc agtctcgcta
     2941 atcttataat tcacgagatg acgcacggta caatctttgt taaaaacaat atgagattta
     3001 acgaaaatat cgcaacattt atcggtgata caggcgccat acttttttta aaaaaaagat
     3061 acggtaacga ttcgcattat ttaaaaaacc atatcaattc gcaacaagac agggatctct
     3121 tttataatca tattatgagc gagacaaaaa aacttaaaga gctatatgaa acctttgacg
     3181 acaccatgac ctacgaacaa aaaaaagaac aaaaagataa tatgataagg tcgattatgc
     3241 aatcaattgc cgatataccc tttaatgatc ccagtaatta tgagaatctg aaaaattatc
     3301 gtcccacaaa cgcatggttt acgggttacc ttacctacag ggcagattta agcaaaatag
     3361 cggatattta ttccgataac tatcattctg acctaagact atttgtagaa gcgcttaaag
     3421 aaaaaaaaga gggtattttg aactaaagcc atattaatca atcgttacaa ttatcttgac
     3481 aatatgtgct tacaataaaa agtattaatt tctgagaatt tcaaataatt gccatgtttt
     3541 aaaatcaatt attgaaattt cagccatttt gcttctataa atttggacaa aattaaacga
     3601 aaaggagaaa accatgaaca ataagaaaac ggtgactatt tttgactcta cacttcgcga
     3661 cggagctcaa gccgaaggta tctccttttc agtaaaagac aagatagata ttttacagac
     3721 acttgatgat ttcggtgtaa cctatatcga agcgggcaat cccggttcaa acccaaagga
     3781 tcttgaattt tttgaaacag caaaaaagat caaactaaag aacagcaccc ttgccgcgtt
     3841 cggcagcaca cgtcgtcgca gcatcaaggt tgaagacgac gcgaatgttc aatcgcttct
     3901 taaagccgaa actccggccg ttgtaatatt cggtaaaagc tgggatttcc atgttacaga
     3961 aatattaaaa gccacacttg atgaaaacct ggagatgatc aaggacacaa tcgagttctt
     4021 caaaaagaaa ggtaaagagg taatatttga cgccgaacat ttttttgatg gatataaaaa
     4081 caatgctgaa tatgcgttaa aatcgctgca ggctgccata gacggcggtg cagactcgct
     4141 ttgtctttgc gaaacaaatg gtggcgctta tcccgatgag gttgcagaaa tagttaacag
     4201 agtagtgact acttttcctg atagtgttat cggaattcat acacataatg acggcggaat
     4261 ggctgtagct aattcggtaa tggctgttgt aaacggtgct acccaggtac agggaacatt
     4321 tattggattc ggtgagagat gtggaaacgc taatctttcg acaataattc cgaacctaga
     4381 gattaaacgc ggttataaat gcctacccga aggaaatctt caaaatctta caagcgtatc
     4441 aaggaaaatt gcttctatcg cgaacatgca attgtatgat tttacccctt ttgtaggcaa
     4501 cagcgccttt gcgcataaag gcggcatgca tattgatggt gttaataaaa actcaaggac
     4561 atttgaacat atagaccctg ctcttgttgg aaacgaacga agatttcttg tgtctgaggt
     4621 tgcaggtcgc agcatgatat taaacacaat acagaagatt gacccatcaa ttgataaaaa
     4681 ttctcccgaa acgaaagaga taatcgatat attgaaaagc atggagcaca atggttacca
     4741 atttgaagga gcagaaagct cttttgaact ggtcattcac cgtcacctga aaacatacaa
     4801 accatttttt caacttgagc atttcaagat tatcggagaa gaaccttcgg cagacaaaac
     4861 tgcgtgtact agcgccatta ttaaagtcaa agttgaccaa acaagtgaga taaccgcggc
     4921 tgagggtgac ggccctgtaa acgctttgga tattgcgctt agaaaggctc ttgaccgttt
     4981 tttcccagaa ctaaaaaatg taaaacttac cgactacaag gtacgagtac tagatacaaa
     5041 aaatgctaca gcagcaaagg tacgggtgct tatcgaatca actgatggca aagagatatg
     5101 gacaacaatc ggggtttcca cagatattat tgaagcgagc tggaaggcgc ttgttgattc
     5161 aattgagtac aaacttttaa ataataattc gtaagatttt tgtttttaaa aataggctta
     5221 attagtggca acctctaaaa attaactttt caacaaggaa cttgcgaaaa attcctttag
     5281 aatagacact taaaccatgt tgccctctgg aacatggtaa tttttcgaag ttccctagtt
     5341 agaatttttg acaaaagagt aattaaaatc aggtaacaga aaaattctat atggagaatt
     5401 aaatcattct ccatatagaa tagtgctgat ggttttaaaa cgtaagacaa tttaatatat
     5461 gataatatat aatcgcccgt ttgaagaata ttttatcttt ggcacctgtt tgtgtcaata
     5521 tatatacaaa aaaacctgcc agacggtctt attttaaaaa taaatcgcta taacatataa
     5581 tcttaatatt gaataattct aactaccacc aatttatact tgaaaatgtg tctgctttaa
     5641 gtatattata acgaagaaaa gactttgaaa ccgcaaacgc ctttgttctt gtcaaagagt
     5701 attagtttaa ttattttcaa agttttaatt tgctcgcatt taatcggtga aactgattac
     5761 atatcattgc tatcattatg tcgcaaaatt agttataatc ccgtattgtt tgggagggag
     5821 tttatgaaaa actttctaat taccgtttta attctgatat ttactgtatc gtgccaaaat
     5881 tcaacagaat ctgaaaaaaa aagcgacgaa cctgttgcaa agaaagctgt tatcaaaatc
     5941 gctttttcta ttggtgaagt taccatcaag gataaaacag acacaaggaa agccatcttg
     6001 ggtgaaattc tggaagaagg ttcaactata ataaccggag aaaaatctac agctgactta
     6061 actttttttg acaccggaat aattcatatc gccgaaaaca gcacactttc tgtttcagaa
     6121 atatcccctg atgctgatgg aacaaccggt cttgatatgc ccaccggaaa agcggctctc
     6181 gcgctttcta agctgaaaaa cggtgatctg aaaataaaaa caccaactat ggttgcggcg
     6241 gtcagaggaa ctgctttcgt aattgaaacg accaagacag ggagcgccgt ttcggttgtt
     6301 cagggggcag ttgaaaccgg aatcattgat tcaggtacgg agaaaagcaa tattacagtc
     6361 atgaccaatg caggtgaaaa gactactgtt aaaacgtccg atatttcggc ctacactagc
     6421 gcaaaaaaag agttaaagcc ggtcaaaatg aaaagacctg acatcgttat agcttcaaaa
     6481 gagttaggac ttattgaagc aaacaggcat gatctggatc cggaaacagt tgaatacatc
     6541 agcgaagagc ctgtaaaatc tattgaaaaa ctattggaag agagtgaaaa aagcttgcca
     6601 tcacctacaa atgataaaga agagagtata gatacttccg agatagacaa ggccgccaga
     6661 gaggcggaag cgaaacggct aaaagaagag agaagaaaaa aacgtatcga ggctgcaaaa
     6721 gaaagtgcta tagcagtacc agcgatgtaa acccacatta ggagattttt ttggatattg
     6781 ctgatatcag gctctactgt tcacaaaaac cgggatcatg ggaagacttc ccttttgacg
     6841 agacgacact agtcataaaa acaggatcaa agatgtacgc tttgatctcc ttaacgaata
     6901 cgcctgtaaa aataaatctt aaatgcgatc ctgaccgctc acagctgtta agacgagatt
     6961 acccttccat aacagaagga tatcacatga ataaacgaca ctggaatacg gttaccagtt
     7021 ctgaagaatc ttcgcatgat gtttctgaca aactgcttaa agagctaatt gaccactctt
     7081 atgatcttgt attttcttct cttacaaaaa aagagaagct tacgattaca aaaaattagc
     7141 atcagatatt ttgccacaaa taagcttgca aacttaccgc tgttttattc tataattaac
     7201 gtctactata gtagcaatta tttttgataa atatttaccc atacatttat agtagaaaaa
     7261 ccatttatct atattttcag gagaacaacc atgaatttca atgaaa
//
LOCUS       DAOTGZ010000142         7183 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_3064
            NODE_12427_length_7183_cov_4.339646, whole genome shotgun sequence.
ACCESSION   DAOTGZ010000142 DAOTGZ010000000
VERSION     DAOTGZ010000142.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868088
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 7183)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7183)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:22:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,785
            CDSs (total)                      :: 2,751
            Genes (coding)                    :: 2,732
            CDSs (with protein)               :: 2,732
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 12 of 19
            Pseudo Genes (internal stop)      :: 5 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7183
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12427_length_7183_cov_4.339646"
                     /isolate="AMR_MDS_3064"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..897
                     /locus_tag="P5123_09465"
     CDS             <1..897
                     /locus_tag="P5123_09465"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HRX16532.1"
                     /translation="NDLQAMIRDTEIDCCSTAFDAISNLNMKAYDLVISEVDLPGDNS
                     FEFYYYLDKNLPYMPVIMTTNRDIDSYFDEIFKNGIGNVLYKPINKQELTGLISKLLR
                     EAPIFGLKNYLPDIVEHKRIRIVSSTQINAAISKITSYIQKWNFEIENMMMMNLILNE
                     IIINAVYHAHGFTQEKLARKKITLPKDKYVDIFFAHNGREYAICVRDYMGTLSSKRIL
                     ESINSVIEQRKTIERAMQEGNFDNFDLAESGRGIELVRKLAKEYYFIIQKGLRTETIL
                     KFDSIHTFESNPITSLKIIERQ"
     gene            complement(956..2155)
                     /gene="ftsZ"
                     /locus_tag="P5123_09470"
     CDS             complement(956..2155)
                     /gene="ftsZ"
                     /locus_tag="P5123_09470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012101728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_process: GO:0000910 - cytokinesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsZ"
                     /protein_id="HRX16533.1"
                     /translation="MLKMQEDRTMNTVIKVVGVGGAGTNAVNRMISTGMEGVEFIVVN
                     TDAQQLSASLAINQVQIGNKLTRGLGAGADPEKGREAANEDRDKIQKLLKGADMVFVT
                     AGMGGGTVTGAAPIIAEIAKELGALVVAVVTKPFKVEGRRRMERAEEGMRNLRDKVDT
                     TITIPNDLLLKIIDKKTPIDEAFKLADDILRQGVQGLADIIQVTGLVNVDFADVRTVM
                     RETGDALMGVGIGHGENKSIDATRMAINSPLLEETSIEGAKAVLINIAGGTDLSLHEA
                     TEVTELITSQVDANANIIFGTTIDPALDERVRVTVIATGFDKKKNALKQKNEHQKGMF
                     ESEKSMNLVQGAFGTELEERKVSGADRAVNYEYLNKVYNNNTKRIYEMPVEDINVPAF
                     LRRGRNS"
     gene            complement(2280..3506)
                     /gene="ftsA"
                     /locus_tag="P5123_09475"
     CDS             complement(2280..3506)
                     /gene="ftsA"
                     /locus_tag="P5123_09475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012645876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0043093 - FtsZ-dependent cytokinesis
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsA"
                     /protein_id="HRX16534.1"
                     /translation="MEDVVVGLDIGSTKTCAVIGFLNENGLVEIAGVGVAPSRGLKNG
                     VIINIDNTQAAIAKAIEDAELMAGCEIAACYVAITGSHIKGENSPGVVAVSGRNRSIS
                     AGEIRRVIQAAQAIVIPADREIMHVLSREFAVDDQRGIKDPIGMTGVRLEAEVHIVTG
                     SRSAIINLTKSVNASGIHCQDIVFSPLAAAEAVLSAEEMELGVAVVDIGGGTTDIAVF
                     SDGGVAYTSVLPVGGRHVTNDISIGLSTPAESAEMIKKKHGLSLVSFVDASETVEVPS
                     VGDRAPRRIMRMELARIIEPRMFEIMEMIERELLVSGKKDFLAAGVVLTGGGAMLEGT
                     VECAERVFRLPVRIGRAKNITGMKDIVSSPQYANAVGLVKYGIKTRQYREAKGGTKKQ
                     SGGLKMKMKKWFQDYL"
     gene            complement(3522..4193)
                     /locus_tag="P5123_09480"
     CDS             complement(3522..4193)
                     /locus_tag="P5123_09480"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRX16535.1"
                     /translation="MKRLILLIFFVLPLNAETESSYQRIRFTGLEYVSKHDIIRSAGV
                     YSKDGRIIVNKEKLDAALKGNSAIADYKIIDNKGILTIVVEENQPEIAVYVVGSGSSR
                     FCEIDNNYNIVTIGEIRSSSTPLIILPVESFEEKRLEEETVKRVENAINLAKSYPVWN
                     QIDSMDFTESNFLRITLKGRESVFEIFEDSYGFERLVACLGYLDTVERYPSLVNLREK
                     IFVFE"
     gene            complement(4194..5567)
                     /gene="murC"
                     /locus_tag="P5123_09485"
     CDS             complement(4194..5567)
                     /gene="murC"
                     /locus_tag="P5123_09485"
                     /EC_number="6.3.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012098698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008763 - UDP-N-acetylmuramate-L-alanine
                     ligase activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate--L-alanine ligase"
                     /protein_id="HRX16536.1"
                     /translation="MIFRNKNKMHFIGIGGVGMSGIAEILINLGYTVTGSDLRESEIT
                     AHLKKAGAEVFVGHYASNVTDQNVVITSTAIDPLNPEIIEARKRSIPIIHRSEMLAEL
                     VRLKHGIGVAGTHGKTTTSSMLSSVLWSCGVNPTSIIGGKVLNFGSNARKGEGDYIVF
                     EADESDGSFLKLLPTIGVVTNIDADHLDYYKYFEGLKDAFLQYINHVPFYGYAVVCID
                     DPAVVSILPKIERPYFTYGISEDADFRASDIRFEDGMTRYNCFFRDEYLGEVALKLLG
                     RHNVVNSLAVVTVARELEIQFDCIQNGLAEFEGVGRRLEILGEAAGVTVIDDYGHHPT
                     EISATLSAVKSLGRRVVTIFQPHRYSRTQLLWDEFGKSFTDTDILFLADIYPASETPI
                     EGVTSALIADSYMKHHGKEAQIIESFEKISGIITDTVREGDIVLTLGAGDIYRCGAEI
                     LKLLEQR"
     gene            complement(5569..6678)
                     /locus_tag="P5123_09490"
     CDS             complement(5569..6678)
                     /locus_tag="P5123_09490"
                     /inference="COORDINATES: protein motif:HMM:NF016027.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine--N-acetylmuramyl-
                     (pentapeptide) pyrophosphoryl-undecaprenol
                     N-acetylglucosamine transferase"
                     /protein_id="HRX16537.1"
                     /translation="MVDKVIMIVGGGTGGHISPGIALYEECNRRKIAVKFLTGRRDKR
                     FKYITDIDREDLLFYDAPALTKNILKLPFFVLRFIIAVIRVKILVKRNGITDVIGMGG
                     YVSGPALFALRKSKINLYLCEQNTVPGRVTTMFAKFAKTIFTTFDDTEDFIKLEYRDK
                     IECVGNPIRSVVLGDISREDARKHYNMQHCDRVILVIGGSQGAVQISELILNMKLTYQ
                     SEFRNIGIIWSTGIESYEHYRKIIHEDNELGAVYLSPFIEDVGIAYHAADIAISRSGS
                     GVMMELAAMKLPAILIPYPYAAMNHQSKNADVFDRSGAAVKIEGKKVNIEEVASVIFD
                     ILGSETRLRQMRRKCAQESRIHAAEDIIANICGGV"
     gene            complement(6692..>7183)
                     /locus_tag="P5123_09495"
     CDS             complement(6692..>7183)
                     /locus_tag="P5123_09495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011524430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsW/RodA/SpoVE family cell cycle protein"
                     /protein_id="HRX16538.1"
                     /translation="RYGSGYHLIQSFIAFKTGGLLGVGLGYGTQKINRLPEPHNDFIF
                     AVIAEETGLVGSVVVILLFTFFFYRGIKIAVSASDDFGRLLAIGLTLNIVIQAYINIG
                     VVTGALPTTGIPLPFISYGGSSLVACMICSGILLNISRYREAAVSEMMLEDPDFVENN
                     DYF"
BASE COUNT         2206 a         1746 c         1110 g         2121 t
ORIGIN      
        1 aatgatcttc aggcaatgat ccgtgacacc gagatcgatt gctgctcaac tgcattcgat
       61 gccataagca atctgaatat gaaagcttat gatctggtaa tatccgaagt tgatcttcct
      121 ggcgataact cctttgaatt ttactattat cttgataaaa atcttccata tatgccagtt
      181 ataatgacaa caaaccggga tatcgatagc tactttgacg aaatttttaa aaacggtata
      241 ggaaatgttc tttataagcc cataaataag caagaactaa cgggtctaat atcaaaactt
      301 cttcgagaag ctcccatttt tggactaaag aactatcttc ccgacattgt tgagcacaaa
      361 agaatacgta ttgtctcctc tacgcagatc aatgccgcca tatccaaaat tacatcttac
      421 atacaaaagt ggaactttga aattgaaaat atgatgatga tgaatctcat tttgaatgaa
      481 attataatta atgcggttta ccatgctcac ggctttaccc aagaaaaact ggcacgaaaa
      541 aaaattaccc tacccaaaga caaatacgta gatatatttt ttgcacataa tggtcgtgaa
      601 tatgctattt gtgttcgtga ttatatggga actctctctt caaaaagaat tcttgaaagc
      661 ataaattctg ttattgaaca gcgaaaaaca attgaacgcg caatgcaaga aggaaacttt
      721 gataactttg accttgctga atcaggccgt ggaattgaac tagtacgtaa actggccaaa
      781 gagtactact ttattattca aaaaggctta agaacagaga ctattctgaa atttgacagc
      841 attcacacct ttgaatccaa tccaataact tcactaaaaa taatcgagcg ccaataataa
      901 aagagaccaa aatggtctct tttatgcgtc caagcaggag gtgcaggaaa aacaactagg
      961 aatttcttcc ccttcttaaa aaagcgggta cgtttatatc ttcaacaggc atttcgtata
     1021 tacgttttgt attattatta tataccttgt tgagatattc ataattaacc gcacgatcag
     1081 cgcccgaaac cttacgctct tcaagctcag taccaaaagc tccctgaaca agattcatgc
     1141 ttttttcact ctcaaacatt cccttttgat gctcattctt ctgttttaat gcgttctttt
     1201 tcttatcaaa acctgttgca attacagtaa ccctgactct ctcatcaaga gcaggatcaa
     1261 ttgttgttcc aaatataata ttcgcgtttg catccacttg gcttgtaata agttccgtta
     1321 cttcggtagc ctcgtgcaac gataaatccg tacccccggc aatattaatc aatactgctt
     1381 ttgcaccttc gatgctagtc tcttcaagaa gaggactatt gatagccatt ctggtagcat
     1441 caatggattt attctctcca tgaccaattc caacgcccat caaagcatca ccggtctccc
     1501 tcataaccgt acggacatca gcaaaatcca cattaaccaa accggtaacc tgaataatat
     1561 ccgcaagccc ttgaactccc tggcgaagta tgtcatcagc caatttaaaa gcttcatcaa
     1621 ttggtgtttt tttatcaatt attttcaaaa gcaggtcatt aggtattgtt atcgttgtgt
     1681 caactttatc acgcaaattg cgcatacctt cttcggcccg ctccatgcga cgacggcctt
     1741 caaccttaaa tggttttgtc actaccgcta ctaccagagc gcccaactct ttggcaattt
     1801 cggcaataat cggagcagct cccgttactg ttccgccccc cattccagcg gttacaaaaa
     1861 ccatatctgc gccttttaaa agtttctgaa tcttgtcccg atcctcattt gcagcctcac
     1921 gacctttttc gggatcagct ccagccccaa gtccgcgagt tagcttattt ccaatctgaa
     1981 cctggtttat cgccagagac gcggatagtt gctgcgcatc cgtattaaca acaataaact
     2041 ccacaccttc catgcccgta gaaatcattc tgtttacagc gttagttccg gctcccccga
     2101 ctccaacaac cttgatgact gtgttcatcg tcctgtcttc ctgcatcttt aacatacgcc
     2161 ccccattaat gtaactattc tatatattat gtctcattat gtcatccaaa aaacgtattt
     2221 gtattaataa aattgtaact tttttttaga ataacatgct attttaatta atcccatact
     2281 taaagatagt cctgaaacca ttttttcatc ttcattttta atccaccact ctgctttttg
     2341 gttcccccct ttgcctctct atattgacga gtctttatac catattttac aagtccaacc
     2401 gcgttagcat actgcggtga agacacaata tctttcattc cggtaatatt ttttgctcgt
     2461 ccaatgcgaa caggcaatct aaaaacacgt tcagcacact caacagtacc ctccagcatg
     2521 gcaccacccc ccgtcaatac tactccagca gccaaaaaat cttttttgcc actaacaaga
     2581 agttcccgtt caatcatctc catgatttcg aacatgcgtg gctcaattat tctggcaagc
     2641 tccattctca taattctacg aggagcacga tctccaacag atggaacttc caccgtctcg
     2701 gaagcatcaa caaacgatac caaagaaagg ccatgcttct ttttgatcat ctctgcagat
     2761 tcagccggtg ttgacaatcc aatcgatata tcatttgtca catgacgccc acctacaggt
     2821 aacaccgaag tatatgcgac accaccatca ctaaacacag caatgtcggt tgttccacca
     2881 cctatatcta caactgcaac accaagctcc atctcctctg ccgataaaac cgcctcagcc
     2941 gcggccaaag gagaaaacac aatatcctgg caatgtatgc cggatgcatt tacactctta
     3001 gtaaggttta ttattgcaga acgcgatcct gtaactatat gaacctccgc ctcaagtcga
     3061 actccagtca tgcctatcgg atctttaatt ccacgctgat catctactgc aaattcacgc
     3121 gagagcacat gcattatttc tctgtccgcg ggtattacta tagcctgagc cgcttgtatt
     3181 acgcgacgta tctctcctgc cgaaattgat cgattgcgtc cagacacagc aacaacaccc
     3241 ggagaatttt cgccttttat gtgactgccg gttatagcga catagcaagc tgctatttca
     3301 caaccagcca tcaactccgc atcctcaata gccttcgcaa tagcggcctg tgtgttatca
     3361 atattaataa taacaccatt tttcaatccc cgagagggag caactcccac tccggctatt
     3421 tcaacaagac cgttttcatt taagaaacct attacagcgc atgtctttgt tgaaccgata
     3481 tcaagaccga ctactacatc ttccatataa accccgataa tctattcaaa aacaaatatc
     3541 ttttccctta aattaaccag cgagggataa cgctctactg tatctaaata tcccaagcaa
     3601 gcaacaagac gttcaaatcc ataactgtcc tcaaaaattt cgaacacgct ttcacgtcct
     3661 ttcaaagtaa ttcttaaaaa attagactca gtaaaatcca tagaatcgat ctgattccat
     3721 accggataac tctttgccaa gtttatggcg ttctctactc tctttactgt ttcctcttcc
     3781 aacctctttt cttcaaaact ttcaaccggt aatattatca gaggagtcga tgaagatcgt
     3841 atttccccaa ttgtaactat gttataattg ttgtcaattt cacaaaacct gctactaccc
     3901 gatccaacta catatacagc aatctctggt tgattctctt ctacaacaat tgttagaatt
     3961 cccttattat caattatctt gtaatctgct attgccgaat ttcctttaag cgctgcatca
     4021 agtttttctt tattaacaat tatccggcca tcttttgaat aaacccctgc tgatcttatt
     4081 atatcatgct tggaaacata ttcaagccca gtaaaacgta tacgctgata actgctctca
     4141 gtttcagcat taagtggtaa tacaaaaaat attagcaaaa ttagacgttt cacctatctt
     4201 tgctccaaca atttgagtat ttcagcacca catctgtaaa tatcaccggc tccaagagtt
     4261 aaaactatat caccctctct aactgtatcg gtaattatcc cagaaatttt ctcaaaactt
     4321 tcaattattt gtgcctcttt accatgatgt ttcatatatg aatctgcaat taacgcggaa
     4381 gtaacgcctt ctattggagt ttcacttgct gggtatatgt cagccaaaaa cagtatatcc
     4441 gtatcggtaa aggattttcc gaactcatcc cacaaaagtt gtgtccgcga atagcgatgt
     4501 ggctgaaaaa tagttacaac tcgtcgcccc aaagatttaa cagcagataa tgtagcagat
     4561 atctctgttg ggtgatgccc ataatcatcg attaccgtca cgccggcagc ctcacccaga
     4621 atttccagtc ttcgccctac accttcaaac tcggccaaac cattttgtat gcaatcgaat
     4681 tgtatctcaa gttctcgagc aacggttaca accgcaagtg aattaacaac attgtggcga
     4741 cctaaaagtt tcagagctac ctcaccaaga tactcatcac gaaaaaaaca gttatacctt
     4801 gtcattccgt cttcgaaacg aatatccgaa gctctaaaat cggcatcttc ggaaataccg
     4861 taagtgaaat atggacgttc tattttaggt aaaattgaaa caaccgctgg gtcatcaatg
     4921 catacaaccg catatccata aaatggcaca tgatttatat attgaagaaa tgcgtctttt
     4981 aacccttcaa aatatttata ataatcaaga tgatcagcat caatatttgt aacaacacca
     5041 atcgttggga gcaatttcag aaaagagcca tccgactcat ctgcctcaaa gacgatatag
     5101 tcaccctcac ctttacgggc attggatcca aaattcaaca ctttaccacc aattattgat
     5161 gtcggattaa caccacagct ccataaaacc gatgaaagca tagaagatgt agtagtctta
     5221 ccatgagtcc cggctactcc gataccatgt ttaaggcgaa ctagttccgc aagcatttct
     5281 gatcgatgta ttatgggaat agatcttttg cgagcctcaa taatttctgg attcaatgga
     5341 tcaatcgcag tagaagtaat tacaacattt tgatcggtaa cgttagaagc gtagtgtcct
     5401 acaaagactt ccgcgccagc cttttttaaa tgtgctgtta tctctgactc acgcaaatca
     5461 gatcctgtaa ctgtatatcc aagatttatc aatatttccg cgataccact cattccaact
     5521 ccgccaatac cgataaaatg catcttgttt ttgtttctga aaatcatatc aaactccgcc
     5581 acaaatatta gctataatat cttccgcggc gtgaatccta gactcctgcg cgcactttct
     5641 tctcatctga cgaagtcgag tttcgctacc aagaatatca aatattacag atgcgacctc
     5701 ttcgatgttt acttttttac cctcaatctt aacagcagca ccagaacggt caaatacatc
     5761 agcattttta ctctgatgat tcattgcggc ataaggatac gggatcaata tagcaggcag
     5821 tttcatagct gcaagttcca tcataacacc tgaaccagat cggctgatcg ctatatcagc
     5881 agcatgatat gctataccga catcttcaat aaaaggagaa aggtaaactg cacccaattc
     5941 attatcctca tgtattatct tacgataatg ctcatatgat tcaatacctg tagaccagat
     6001 aatacctata ttacggaatt cagattgata agtaagcttc atgttaagta tcaattcact
     6061 aatctgcaca gctccctggc ttccaccaat cacaagtatc actctatcgc agtgttgcat
     6121 attgtaatgc ttgcgggcat cctccctgct aatatcacca aggacaacag accgtatagg
     6181 attacctaca cactcaattt tatcacgata ttcaagttta ataaagtctt ccgtatcatc
     6241 aaaggttgta aaaattgtct tcgcaaattt tgcaaacatt gtggttaccc gaccaggaac
     6301 agtattttgc tcacataaat agagatttat tttagatttt cttaacgcga acagagccgg
     6361 gcccgagaca tatccaccca tgccaataac atcagtgata ccgtttcgct taacaagaat
     6421 ctttactcga atcacagcaa taataaatct gagaacaaaa aacggaagtt taagtatatt
     6481 cttagtcaat gcaggagcgt catagaataa caaatcttct ctgtcaatat cggttatgta
     6541 cttaaatctt ttatctctac gccctgtcaa aaatttgact gcaatcttac gcctattgca
     6601 ctcctcatac aaggcaattc cggggctaat atgtccacct gtcccgccac cgactatcat
     6661 tattacctta tcaaccattt tatactcccc tctaaaaata atcgttattt tctacaaaat
     6721 caggatcttc caacatcatt tccgacaccg cagcttcccg gtagcgggat atatttaata
     6781 atattccgga acatatcatg cacgctacta gcgaagatcc tccatagctt ataaatggca
     6841 atggtatacc agttgtagga agtgccccgg ttacaactcc tatatttata tatgcctgaa
     6901 taactatatt cagagtcaat ccaattgcca ataatcttcc aaaatcatcc gaagcactta
     6961 ctgcaatctt tatacctcta taaaagaaaa atgtaaagag caaaattacg acaactgacc
     7021 cgactagccc agtttcttcc gcaataacag caaaaataaa gtcgttatgc ggttcaggca
     7081 gtctgttgat cttttgtgtt ccatatccaa gaccaactcc aagcaaccca cctgttttaa
     7141 atgcaataaa agactgaata aggtggtatc cgctaccgta tct
//
LOCUS       DAOTGZ010000143         7099 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_3064
            NODE_12614_length_7099_cov_4.443782, whole genome shotgun sequence.
ACCESSION   DAOTGZ010000143 DAOTGZ010000000
VERSION     DAOTGZ010000143.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868088
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 7099)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7099)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:22:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,785
            CDSs (total)                      :: 2,751
            Genes (coding)                    :: 2,732
            CDSs (with protein)               :: 2,732
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 12 of 19
            Pseudo Genes (internal stop)      :: 5 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7099
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12614_length_7099_cov_4.443782"
                     /isolate="AMR_MDS_3064"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..311)
                     /locus_tag="P5123_09500"
     CDS             complement(<1..311)
                     /locus_tag="P5123_09500"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRX16539.1"
                     /translation="MLRFFLKGSRDHKGRIQDFAVLLGLFVFSTVLILGLLPKEESSA
                     TSLNTFFMNVMMVVPVIIAVYFIVISFRRKVSTTVSQVSTSIRTKIMLSLLFVAIVPT
                     LP"
     gene            complement(311..574)
                     /locus_tag="P5123_09505"
     CDS             complement(311..574)
                     /locus_tag="P5123_09505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018248728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HPr family phosphocarrier protein"
                     /protein_id="HRX16540.1"
                     /translation="MVEKKVVIQGESGIHARPATMLVKTASKYSCNVFIEKDGTDANL
                     KSILNVLALGLTGGTEIKIKAEGEGENEAVEEIVALIERDFAE"
     gene            complement(567..1517)
                     /gene="hprK"
                     /locus_tag="P5123_09510"
     CDS             complement(567..1517)
                     /gene="hprK"
                     /locus_tag="P5123_09510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013314962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0016791 - phosphatase activity [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HPr(Ser) kinase/phosphatase"
                     /protein_id="HRX16541.1"
                     /translation="MTQKLFLRDLLSNPEDTQLQLLPLTGELGLGREIKSADVNRPGL
                     SLAGFFDHFAADRIQVFGKGESAYIDSLSEKKLHESLDNFFSCNVFCAVFTHGKKPPY
                     LFTELALQKGTPVFVSSLTTAEFIRLFHYVVSETHAPQKNVHGTLVDVFGLGVLIIGA
                     SGVGKSEAALELIERGHRLIGDDIVNVKWVKESMLVGQGSRFIRHHMEIRGLGILNVT
                     DVFGIGSVRNRKRIELVVKLELWDDDKEYERLGLYDTRYELLGMEVPYVLLPVRPGRN
                     IPILIESAALNQRLKKMGVYSAQELDRRIQDWIKVGKNDG"
     gene            complement(1520..2305)
                     /gene="raiA"
                     /locus_tag="P5123_09515"
     CDS             complement(1520..2305)
                     /gene="raiA"
                     /locus_tag="P5123_09515"
                     /inference="COORDINATES: protein motif:HMM:TIGR00741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_component: GO:0022626 - cytosolic ribosome [Evidence
                     IEA];
                     GO_function: GO:0045182 - translation regulator activity
                     [Evidence IEA];
                     GO_process: GO:0009386 - translational attenuation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome-associated translation inhibitor RaiA"
                     /protein_id="HRX16542.1"
                     /translation="MKLKITGRHVTVSDKLKEHVEKKVSKVEKYFNQIISIEVIVFEE
                     KSDKVVEIIIHADGAKFYTSDKSSDYYSCLDSLIDKLDQQIKKYKDKLQMHKGPGTNF
                     IPIVDLDKDGHESVAVVEASPKPLNYIEAYLELKMDNKGFILYKKDNENIDYASRNYA
                     LLYEDDKKYVMVEVPDEMLKNRSFSGDLNCFELDIKNESPSDPKIDLKATRKDIAALN
                     VSQAIAEHEKSDRKLFPFYNTESSSLNLLVKRSNQLEIVVPAQ"
     gene            complement(2310..3740)
                     /gene="rpoN"
                     /locus_tag="P5123_09520"
     CDS             complement(2310..3740)
                     /gene="rpoN"
                     /locus_tag="P5123_09520"
                     /inference="COORDINATES: protein motif:HMM:TIGR02395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase factor sigma-54"
                     /protein_id="HRX16543.1"
                     /translation="MAVSLSIGLKQTQRLAMTQSLRQSIEMLQLSSLELAETISHELE
                     SNPVLEEVSLSRETEYSSELVHDMNLQLSGDTSRADRIEQRDRDFGNTSDAGFQKSYD
                     EDRKQQAIENAFVERESLADHLISQVRLLKGSEQELSFLETLVSLLDSNGFFTEDHES
                     FCAEQSVTPEYLDLSLRKISRLEPIGCGSSSVQQSLLWQCENKFPADELLYEMIRDHF
                     DLLSKLFFDQISKKMDVPLDTIIKKSNLLQNFSPYPGRMFSRNETNYVIPELEVHYID
                     DQIVLMFYDDLVPGIKINKYYTGLVESSKVDKEIKKYLRECITSAKYLLRSISGRRET
                     IEKVVRAIMECQTEFLKKGPGHLKPLIHANIAEITGFNESTISRVTSNKYVQTVWGIF
                     ELKYFFVTRINSDDNEQSSDNVQKLIQDIVTNEDPLNPFTDEQIVTRLDNAGIQTARR
                     TVAKYRGILNIPSSTKRKRINKLKME"
     gene            complement(3790..5400)
                     /locus_tag="P5123_09525"
     CDS             complement(3790..5400)
                     /locus_tag="P5123_09525"
                     /EC_number="6.3.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011172205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003883 - CTP synthase activity [Evidence
                     IEA];
                     GO_process: GO:0006221 - pyrimidine nucleotide
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0006241 - CTP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CTP synthase"
                     /protein_id="HRX16544.1"
                     /translation="MTDKTKFIFITGGVCSSLGKGISTASLGILLEGHGYKISILKMD
                     PYLNIDPGTMSPYQHGEVYVTEDGAETDLDLGYYERFTNSELTRKNSVSTGQIYLSVI
                     ERERRGDYLGRTVQVIPHITNEIKKRIFDVSKSGDLDFVLVEIGGTVGDIESVPFLEA
                     IRQMRMDLGKNNVLFVHLTLVPAITVAGEHKTKPTQHSVKELMQTGILPDILLCRSSR
                     PLDDEMKAKIALFCNVEDGNVISALDSDSIYDIPMMLHGFKYDDIVLEHFNKPKRELD
                     ISEWLEFVDHHNNPEKDVTIGIVGKYVSLQDAYKSIYEALIHGAAYNRARLKLVKIDP
                     EELEGCDGSIDKVFSGVDGVLVPGGFGSRGIEGKIKAIEYARINNIPFFGICLGLHTA
                     VIEFARNVCGLEDANSTEFNPECKNPVISLMEDQKIVQDKGASMRLGAFACKLLSGSG
                     IADIYQKQLIHERHRHRYEFTSRYKKIFEDAGMVFGGINPDLDLVETLELPEHRWFYA
                     VQFHPEFKSKPTAPHPLFKTFITASVLK"
     gene            5706..6092
                     /locus_tag="P5123_09530"
     CDS             5706..6092
                     /locus_tag="P5123_09530"
                     /inference="COORDINATES: protein motif:HMM:NF018894.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0035438 - cyclic-di-GMP binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PilZ domain-containing protein"
                     /protein_id="HRX16545.1"
                     /translation="MHEKRQYHRYPCLLKVRFQYHDGNPEDPYQMAKKPQKGKGRILD
                     ISRGGIFIASYTKTGINLPIRILFKTNNRIISQTGTIVRTGLIRNNPAEVLKKFASLD
                     ISEDAYLAIMFNEPLEDFDSDEITIL"
     gene            6089..7075
                     /locus_tag="P5123_09535"
     CDS             6089..7075
                     /locus_tag="P5123_09535"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRX16546.1"
                     /translation="MRTDFIHIDSIEDIDTSKASVYDLNKRYMDKNGTMYGLRFNRSQ
                     RKVEIIRILRTSNDRAQMIRHRIREKKKQDMKELAELGDIPENSDDQLIEDEIGKENE
                     IDDYYTENNDDSDSDIAATGETMEPGSELSTIETELKNFNPDHFIKEIFDTINTHRDR
                     FNGVINNIHNSKMISPDDRELSNMLEDTHRSLDIDGFQKIEDIISIYREINEYPRPIS
                     FYITHLDPRCRDILNQMQTDKQKYHYILLYEMDQHLKHVYKKLLGFADSLQKIIDEIE
                     MNMASKINALARQNLKDATFTLETTIGEIKTVLTKLTTLEQYIKTERNFIQQ"
BASE COUNT         2248 a         1562 c         1238 g         2051 t
ORIGIN      
        1 ggtaaagtgg gtactattgc gacaaaaaga agtgacagca taatttttgt tctaatggat
       61 gtactgacct gacttacggt agtactgact tttcgcctaa aagaaataac aataaagtat
      121 acagcaataa taaccggcac caccatcatt acattcatga aaaatgtatt taacgatgta
      181 gctgaacttt cttccttagg aagcaagcca agtatcaaca ctgtagaaaa aacaaaaagc
      241 cccagaagaa ccgcgaaatc ctggatacgc cccttgtgat cacgactgcc ttttaaaaaa
      301 aaccttaaca ttactcggca aaatcgcgtt caatcaaagc aacaatctct tcaacagcct
      361 cgttttcacc ttcaccttct gctttgatct ttatttcagt tccacccgtt aaccccaacg
      421 cgaggacatt aagtatggat ttgaggttcg cgtctgttcc atctttttct ataaaaacat
      481 tacaactata cttggatgct gttttaacaa gcatggtagc gggtctcgcg tgaatacccg
      541 actcaccttg tattactacc tttttttcaa ccatcgttct ttcctacctt tatccaatcc
      601 tgaatacgcc tgtcgagttc ctgagccgaa tagaccccca tctttttcaa acgctgattt
      661 aaagcggcgc tttctattaa tatcggtata tttcgaccag gtctcactgg gagcaaaaca
      721 tagggtactt ccattcccag cagttcatac ctggtatcgt aaagccccag tctctcgtat
      781 tctttgtcat catcccacag ctccagtttt actacaagct caattctttt ccggtttctt
      841 acagaaccaa taccaaacac atccgtaaca ttaagtatgc cgagacctct aatttccata
      901 tgatgacgga taaaacgact cccctgcccc accagcatcg actccttgac ccattttaca
      961 ttgactatat catccccaat aaggcgatgt ccacgttcta taagttcaag ggcagcttca
     1021 cttttaccaa ccccgcttgc accgataatc aaaacaccaa gaccaaacac atcaacaaga
     1081 gttccatgca catttttctg tggagcatgc gtctccgaaa cgacatagtg gaacagacgt
     1141 ataaactctg cggtggttaa cgagctgaca aaaaccggag tacccttctg taatgccaat
     1201 tcagtaaaaa gataaggtgg ttttttacca tgcgtaaaaa ccgcacaaaa gacattgcac
     1261 gaaaaaaaat tatcaagtga ttcatgcaac tttttttcag agagtgaatc gatatacgca
     1321 ctttcacctt ttccaaacac ctgaattcta tcggctgcaa aatggtcaaa aaaaccggca
     1381 aggctgagcc ccggccggtt tacatcagca cttttaattt ccctgccaag ccccagctcg
     1441 ccggtaagag gcaaaagctg gagctgtgtg tcctcagggt ttgaaagaag atctcgcaaa
     1501 aacagttttt gagtcatatt tattgagccg gtacaacaat ttccagctga tttgaacgtt
     1561 taaccagcag attcaaagac gatgactctg tattgtaaaa aggaaacagt tttcgatcgc
     1621 ttttttcatg ttcagcgata gcctggctaa catttaaagc agcaatatct ttacgtgtcg
     1681 ctttaagatc gatctttgga tcagatggcg attcgttttt tatatccagt tcaaaacagt
     1741 tgaggtcacc cgaaaatgat ctatttttaa gcatctcatc gggtacctct accataacat
     1801 attttttatc atcttcgtaa agcagggcgt agtttctgct cgcataatct atattttcgt
     1861 tatctttttt gtaaagaatg aatcccttgt tatccatctt cagttcaaga tatgcttcaa
     1921 tatagttaag cggtttaggc gaagcctcaa caacagctac agactcatgt ccatccttgt
     1981 ccagatcaac aatcggaata aagtttgtac ctggaccttt atgcatttgc aatttgtcct
     2041 tatatttttt aatctgctga tcaagcttat caatcaaaga atcaagacag ctgtaataat
     2101 cagaagattt gtcggaagta taaaacttgg ctccgtctgc atgaattatt atctcaacaa
     2161 ctttatcact cttttcttcg aatacaatca cctcgataga gattatttga ttaaaatact
     2221 tttcaacttt tgagactttt ttttccacat gttccttgag tttgtcagat actgtcacat
     2281 gtcttccggt aatttttagt ttcatacgct cactccattt ttaacttatt tattctcttc
     2341 ctctttgttg aagagggaat gttaagaatt cctcgatatt ttgccacagt ccgccttgca
     2401 gtctggattc ctgcattatc taagcgggta acaatttgtt catcggtaaa gggattcaag
     2461 ggatcttcat tcgtaacaat atcctgaatc aacttctgta cattatccga cgactgctcg
     2521 ttatcatcag aattgattcg tgtaacgaag aaatatttta attcaaagat gccccatact
     2581 gtctgaacat atttatttga agttactcga cttattgtcg actcgttaaa acctgtaatc
     2641 tctgcaatat tagcgtgtat caaaggctta agatgtcccg gccctttttt aagaaactcg
     2701 gtttgacact ccattatggc acgcacaact ttctctatgg tttctcgtct tccggatata
     2761 cttcttaaaa gatatttggc agaagtaata cattcacgca ggtatttctt tatctctttg
     2821 tcaactttgg aagattcaac caaaccggtg taatacttat taatttttat tcccggtacc
     2881 agatcatcgt aaaacattaa tactatctga tcatcaatat agtgaacctc aagttcggga
     2941 ataacgtaat tggtctcgtt tcttgaaaac atccgacccg gatagggtga aaagttttga
     3001 agtagattac tttttttgat tatcgtatca agaggtacat ccattttttt tgatatttga
     3061 tcaaaaaaaa gttttgaaag cagatcaaaa tgatcccgaa tcatttcgta aagtaattca
     3121 tcagccggaa atttgttttc acactgccac aaaagagact gttgaacaga tgaagaaccg
     3181 cagcctatgg gctcaagacg ggatattttt cttaaagaaa ggtcaagata ttcaggagtt
     3241 acagactgct cggcacaaaa agactcatga tcctctgtga aaaatccgtt agaatcaagc
     3301 agtgacacaa gagtttctag aaaagaaagc tcttgttctg atcctttcag aagccgaacc
     3361 tgagatataa gatgatccgc caaagattca cgctctacaa atgcgttttc aattgcctgc
     3421 tgttttcgat cctcgtcata acttttttga aaacccgcgt cactagtatt accaaagtct
     3481 cgatcacgct gttcgattcg atctgcacga gaggtatcac ccgaaagctg tagattcatg
     3541 tcatgaacca gctcactgct atattcggtc tctcgggaca aagagacctc ctccaaaaca
     3601 ggatttgact ccagttcatg cgaaatggtc tcagccaact caagggagga gagctgaagc
     3661 atttcgatgg attgacgtaa gctttgcgtc atggcgagac gctgagtttg ctttaagcct
     3721 atggaaagtg acactgccat attcaacctc tgccacaaag gtacaacaga atatctataa
     3781 tttcaagctt tattttaaga cagaagcggt tataaatgtc ttaaaaaggg gatgaggcgc
     3841 agttggtttt gatttaaact caggatgaaa ctgaacagcg taaaaccaac ggtgttcggg
     3901 caattcaaga gtctccacaa gatccagatc gggattgata ccgccgaaga ccatgcccgc
     3961 atcttcgaaa atctttttat acctagaagt aaactcgtac cgatggcggt gcctttcgtg
     4021 aatcagctgt ttctggtata tatcagctat accgcttcca gaaaggagct tgcaagcaaa
     4081 tgctccaaga cgcatggaag ctcccttgtc ctggactatt ttctgatctt ccatcaggga
     4141 aataacggga tttttacatt cgggattaaa ttcggtcgag ttggcgtcct ctaatccgca
     4201 aacatttctg gcgaactcaa taaccgctgt atgaagcccc aaacagatac cgaagaaagg
     4261 aatattattt atacgggcat attctatcgc ttttatctta ccctctattc ctctgctacc
     4321 gaaacctcca ggcacaagaa ccccgtctac tccactaaaa accttatcaa tagagccatc
     4381 acagccctca agctcttcag gatcgatttt cacaagtttt aaccttgccc tattatatgc
     4441 ggcaccatga ataagagctt cgtatattga cttataagcg tcctgaagag aaacatattt
     4501 gcctacaata cctattgtta catccttttc cggattgtta tggtggtcaa caaattcaag
     4561 ccattcagaa atatcaagtt ctctctttgg cttattgaaa tgctcaagaa caatatcatc
     4621 atacttaaaa ccgtgcaaca tcataggtat atcataaatc gaatcactat caagagccga
     4681 tatgacattt ccgtcttcaa cattacaaaa aagtgctatt ttagctttca tctcatcatc
     4741 tagaggccgg gaagaacgac ataataaaat atctggaaga atacctgtct gcataagctc
     4801 ttttacagag tgttgagtcg gtttagtctt gtgctcaccc gcaacagtta ttgcaggaac
     4861 aagcgtaagg tgtacaaaca acacattatt tttacctaaa tccatacgca tctgccggat
     4921 agcttcaaga aaaggaacag actcaatatc ccccactgta ccacctattt cgaccaaaac
     4981 aaaatccaga tctccggact tggacacatc aaaaattcgc tttttaatct catttgtaat
     5041 atgcggaata acctgtacgg ttcgcccaag ataatcgcct ctacgctctc tttcaatgac
     5101 cgaaagatat atttgaccgg ttgatactga atttttacga gtaagctctg agtttgtaaa
     5161 tcgctcataa tatccaagat caaggtcggt ctcggccccg tcttcggtta cgtatacttc
     5221 accatgctga taaggactca ttgtaccggg atcaatattc agatatggat ccatcttcaa
     5281 aatagaaatt ttatacccat gtccttccag gagtattcct aatgaggctg tagatatgcc
     5341 tttcccaaga gaagagcaga ctccaccggt aatgaaaata aactttgttt tatcagtcat
     5401 aagagttacg tctccgatta tataaagata agaacccttg aaaatgtttt taatgtcggg
     5461 tacaggtttt ttcaaaagaa ttttatgcta ctcctttcgt caatgagctt ttacggacaa
     5521 aaaaagtata accgctaatt taacattacc ttaacaatgc taaattaacg tcaaggtttg
     5581 attaacccag taaagggttt gacaaaattg ttggcaccat acatgctttg aaccggaatt
     5641 actttatccg ctacacttta aggtgataga tttatacaaa aatccggtaa atcgggagct
     5701 tcaaaatgca tgaaaaacgc cagtatcatc gctacccctg tctgttaaaa gtaagattcc
     5761 aatatcacga tggcaacccc gaagacccct atcaaatggc taaaaagcca caaaagggaa
     5821 agggtcgaat ccttgatatc agtcgcggcg gtattttcat agcctcttat accaaaacag
     5881 gaatcaattt accaataaga atccttttta agacaaacaa ccgaattatt tcacaaactg
     5941 gtacaattgt ccgaacaggt cttataagaa acaatccggc cgaagttctt aaaaaatttg
     6001 cttccttgga tataagtgaa gatgcctatc tggcaatcat gtttaatgaa cctcttgaag
     6061 atttcgacag tgatgaaata acaattttat gagaacagat ttcatacata tcgactctat
     6121 cgaagacatt gatacatcca aagcgtctgt ttacgacctt aacaaaagat acatggataa
     6181 aaacggcaca atgtacggtc ttcgtttcaa ccgttcacag cgcaaggtcg agattatccg
     6241 aatactgcgt acttccaacg atcgtgctca gatgatacgc cacagaatac gtgaaaagaa
     6301 gaaacaagac atgaaagaac ttgccgaact aggggatatt ccagaaaata gtgatgacca
     6361 gctaatcgag gacgaaattg gaaaagaaaa tgaaatagac gattattata cggaaaataa
     6421 cgatgatagt gattctgata tagccgcaac aggcgaaacg atggaacccg gatcagagtt
     6481 aagtacaatt gaaacagaac tgaaaaattt taaccctgac cattttatca aagaaatatt
     6541 cgacacaatc aatactcacc gtgaccgttt taatggagtg ataaacaata ttcacaactc
     6601 gaaaatgata tcacccgacg accgcgaact ctcaaacatg cttgaagaca cacatcgttc
     6661 acttgacatt gacggatttc aaaaaattga agatataatc tcaatttaca gggaaataaa
     6721 cgaatacccg cgccctattt ctttttatat cacgcaccta gatccccgct gccgtgatat
     6781 tttaaatcag atgcaaacgg acaaacaaaa atatcattac atacttcttt acgagatgga
     6841 ccaacacctt aaacatgttt acaagaagtt attggggttt gccgattctc tgcaaaagat
     6901 aatcgacgag attgaaatga atatggcttc taaaataaac gctctggcgc gtcaaaacct
     6961 caaagacgcg acatttactc ttgaaaccac tatcggagag ataaaaacag ttttgacaaa
     7021 gctaacaaca cttgaacaat atatcaaaac cgaacgcaac ttcatacaac aatagggaca
     7081 ccttcatgga cataccaga
//
LOCUS       DAOTGZ010000144         7079 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_3064
            NODE_12655_length_7079_cov_5.163155, whole genome shotgun sequence.
ACCESSION   DAOTGZ010000144 DAOTGZ010000000
VERSION     DAOTGZ010000144.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868088
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 7079)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7079)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:22:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,785
            CDSs (total)                      :: 2,751
            Genes (coding)                    :: 2,732
            CDSs (with protein)               :: 2,732
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 12 of 19
            Pseudo Genes (internal stop)      :: 5 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7079
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12655_length_7079_cov_5.163155"
                     /isolate="AMR_MDS_3064"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..463)
                     /locus_tag="P5123_09540"
     CDS             complement(<1..463)
                     /locus_tag="P5123_09540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009224277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRX16547.1"
                     /translation="MAYRELDCYQYIEDQAAKLAIRNLIAINLQFKEITMYKKFLQTV
                     LISLVTVTLLLSCSKEKSTEKDNGQLLLKPGEPLVTDNFTADPSAHVFEGKLYIYPSH
                     DIDTGVADSTDGSHYDMKDYHVYSITDFNSPITDHGTVLKLEDIPWASKQLW"
     gene            complement(692..2014)
                     /locus_tag="P5123_09545"
     CDS             complement(692..2014)
                     /locus_tag="P5123_09545"
                     /EC_number="2.7.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013726120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004072 - aspartate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate kinase"
                     /protein_id="HRX16548.1"
                     /translation="MSKRVVCKFGGSSVADTEQFKKIKNIINSDSKRKIVVVSAPGKR
                     NSKETKLTDLFYSTYDLATKCVDISGPWSLICERYLEITKELGLKTEINGDLLKLEKQ
                     LRETPEEVTSDYLVSRGEFLCARIMAEYLEAEFVDSYPLIKFDSRYRVLDQSYRDIRE
                     SLSDDSKIYVVPGFYGSDTKGNLKTFTRGGSDITGAILAKACDAEVYENWTDVSGLLM
                     ADPRIVDNARPMAYVSYREIRELSYSGASVLHDEAIAPCRDKDIKINIRNTNFPEDSG
                     TMIGPEPEGAKQVITGIAGRTAFSMIHIEKSMMNKERGFGRKVLGILENHNLSYEHSP
                     TGIDSMSIVLDSKSFADVEDDVLEDIRLEMNPDSIKVIDNLALIATVGHGMAHKVGVA
                     AKLFGALAEEKINVRVIDQGSSELNIIIGIDENDYEKSIKAIYKAFVD"
     gene            complement(2141..3313)
                     /locus_tag="P5123_09550"
     CDS             complement(2141..3313)
                     /locus_tag="P5123_09550"
                     /inference="COORDINATES: protein motif:HMM:NF019310.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HRX16549.1"
                     /translation="MEKQISFTSKILTVIASFAIMLCIGGVYAWSIFASELRTAYGLS
                     STQTQLVFGLLIAVFPVTMIIAAKIEKKIGTRGIVLLSALFMSAGYFLASLSHGNIFI
                     LIIGIGVLAGAGTGFGYLAALTSPVKCFPEKKGMITGIASAGFGLAAVILSSVAETML
                     NNGINVLQVFRYIAATYLVLILFFTFMISSPYSATGYNGLSLRLLLKAPKFYLLFIGI
                     FMGTFAGLTIVGNLKSIGGVYQIDNHVLVIGVSVFAIANFLGRILWGIVSDYTGAHRA
                     LTVALFFQGASILLMGILKLNGITYLTLCASAGFGFGSNFVLFAKESAQFFGLENLSV
                     VYPYVFLGYALAGLTGPSTGGMIFDLTGSYFIAIIICAGLCFTGGLIFLLNRARPE"
     gene            complement(3464..5359)
                     /locus_tag="P5123_09555"
                     /pseudo
     CDS             complement(3464..5359)
                     /locus_tag="P5123_09555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005489898.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4914 family protein"
     gene            complement(5601..5951)
                     /locus_tag="P5123_09560"
     CDS             complement(5601..5951)
                     /locus_tag="P5123_09560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014450000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2294 domain-containing protein"
                     /protein_id="HRX16550.1"
                     /translation="MTKGQIEAQISDTVIRFEKEYMGRGPIETKTTIIDDMVVVRLKG
                     VLTPAEEKLAQNPDGAGLIKQVRMQLLESAKAMLQVMVGDVTQKKVLSLHTDISTKTG
                     ERIIVFILNGKFEI"
     gene            6248..>7079
                     /locus_tag="P5123_09565"
     CDS             6248..>7079
                     /locus_tag="P5123_09565"
                     /inference="COORDINATES: protein motif:HMM:NF013697.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HRX16551.1"
                     /translation="MKHRITFFIVSAFFVIIFIISLSCNKKDSEAKPITIRWWHINSD
                     KQSQVVFQNIANEFEKTNPDVIIKITMLDNMEYKPKLELDFAAGDPPDIFHSWGGGGL
                     AEQVEAGHLRDITSYVKSDNWQSKISPIALEIYSHNGKIYGFPHDLGAVGFWYNKELL
                     KKVGYTSFPETWHDFYKLCDRLKKSGITPISIGIADRWPVMYYWVYLAMRIGGEDLFS
                     DIHKGNIKFNNPDLIKAGLIMQDLFKHGYFQSSSIGDDFITQSRYMGDGECAMQLMGQ
                     W"
BASE COUNT         2190 a         1496 c         1211 g         2182 t
ORIGIN      
        1 cccagagctg ttttgaagcc cagggtatat cttcaagctt cagaactgtg ccatgatcgg
       61 ttatcggaga gttgaaatct gttatagaat agacatggta atccttcatg tcgtaatggc
      121 ttccatcggt tgaatctgca acccctgtat ctatatcgtg agagggataa atatagagct
      181 taccttcaaa aacatgcgcg gagggatcgg cagtaaagtt atctgtaaca agaggctcgc
      241 ctggtttaag tagcaactga ccgttatctt tttcggttga tttttctttg ctacacgata
      301 atagtaatgt aacggttacc aaagaaatta aaacagtttg taaaaatttt ttgtacatgg
      361 ttatttcctt aaattgtaaa ttaattgcaa ttagatttct tattgccagt ttagcagcct
      421 gatcttcgat gtattgataa caatcaagct ctcgataagc catcaacttt tgaaacccct
      481 ttgtaaaagc tgattataaa ggcagtataa aaaaagctct atttttatca agactattat
      541 aaaatccgat tactgcttag agtgttaaaa aaatagccct tcaataaact ttactgttaa
      601 gaatatcatg gtgtaacaat tttttttcaa ataatcccga aagaaactca ctctgtttag
      661 tagagggttt ctttcggatt aataaatatt cttaatccac aaaagcctta tagattgcct
      721 ttatcgattt ttcatagtcg ttttcatcta taccaatgat aatgtttagc tctgatgaac
      781 cttgatcaat taccctaaca ttaatttttt cttctgcaag agcaccaaaa agtttcgcgg
      841 caacccccac cttgtgagcc attccatggc caaccgtagc aattagcgca agattatcta
      901 tcacctttat actgtccgga ttcatttcca gacgaatatc ctctaacaca tcatcttcta
      961 catctgcaaa tgattttgaa tccaatacga tactcataga gtcaataccg gttggagagt
     1021 gctcgtatga gagattatga ttttcaagaa taccaagtac cttgcgtcca aatcctctct
     1081 ctttattcat catgctcttt tctatgtgta tcattgaaaa agctgttcga ccagctattc
     1141 cggttattac ctgtttagca ccttcaggtt ctggtccgat catagtgcca gaatcttccg
     1201 gaaagtttgt attacgtatg ttgattttga tatctttatc acggcatggt gcaatagcct
     1261 catcatgtaa aacactggct ccgctgtacg atagctctct tatctcgcgg taactgacat
     1321 aagccatggg acgcgcgtta tcaacaatac ggggatcggc cataagaagc ccggatacat
     1381 ctgtccagtt ttcataaact tcggcgtcac aagccttggc aagtattgct cctgtaatat
     1441 cggaaccacc tcttgtaaaa gttttaagat taccctttgt atctgatccg taaaatcccg
     1501 gaacaacata gattttactg tcatccgata atgactccct aatatcacga tacgactgat
     1561 caagaactct gtaacggctg tcaaacttta tcagtggata agaatccaca aattcggcct
     1621 caagatattc ggccataata cgtgcacata aaaattcccc tctactgacc agataatcag
     1681 aagttacctc ttccggagtc tcccgaagct gtttttccag ttttaacaaa tcgccgttta
     1741 tttcggtttt taatccaagc tcctttgtaa tttcaagata acgctcacaa ataagcgacc
     1801 atggtccact aatatcaaca catttagtag ctaaatcata tgttgaatag aagagatccg
     1861 ttaattttgt ctctttggaa tttctcttgc caggtgccga aaccacaaca attttacgct
     1921 tactgtcgga attaattata tttttaattt ttttaaactg ctctgtatct gctacagaac
     1981 ttccaccgaa tttgcatacc actctttttg acatatcacc ttcccttttt taagaatttt
     2041 taggtttagc atcaggatgt ttaccaattg agaacttcaa aaattaccgt ttaaacagat
     2101 tcatatactg ctgatatatt gccaaatact ttttaattga ttattcaggc cttgccctat
     2161 ttaataagaa aattaggcct cctgtaaaac agagccccgc gcaaataatt atagcaataa
     2221 aataactacc tgtaagatca aaaatcatac caccggttga aggtcctgtt aatccggcaa
     2281 gggcgtaacc caagaaaaca tatggatata caacagaaag attctcaagc ccgaaaaact
     2341 gggcactctc ttttgcaaag agcacaaaat tggatccaaa accaaacccc gctgaagcgc
     2401 atagagtaag atatgtaatg ccatttaact tcaaaatccc catcagaaga atagatgcac
     2461 cctggaaaaa aagtgcaact gtcaaagcgc gatgtgctcc tgtatagtca ctgacaattc
     2521 cccacaatat tcttccaaga aaattggcaa tcgcgaagac tgacactcca atgacaagga
     2581 catgattatc aatctgatat acaccaccaa tagatttcag gttcccaact atggttagac
     2641 cagcaaaagt gcccatgaaa attccaataa aaagaagata gaactttgga gccttcaaaa
     2701 gcagacgcag actcaaacca ttataaccgg tcgcagaata gggagagctg atcatgaatg
     2761 tgaaaaagag aattaaaaca agataggttg cagcaatata tctaaatact tgtaatacat
     2821 taattccgtt atttaacatt gtttccgcaa cagaagagag aattacagcc gctagcccaa
     2881 atcccgcaga agcgatacca gtaatcatcc ccttcttttc tggaaagcat ttaaccggag
     2941 aggttaatgc cgccaaatat ccaaatccag tacctgctcc tgcaagaaca ccgattccga
     3001 taattaaaat aaaaatatta ccgtgtgata aagatgctaa aaaatagccc gcactcataa
     3061 atagggctga aagcagtaca attcctcttg tacctatttt tttttcaatt ttggcagcaa
     3121 taatcattgt taccgggaaa accgcgatca gaagaccgaa tacaagctga gtctgtgttg
     3181 aactaagtcc ataagcagtt ctaagttcag aagcaaatat actccatgcg taaacaccgc
     3241 ctatacacag cataatcgcg aaagaagcta taaccgttaa aatttttgat gtaaagctta
     3301 tctgtttttc caatattgat ccacctctta taattgggtt ttaaccggct ctgcttttct
     3361 atcagtgtaa tccatttatc cgaaccggta ataatttatt taattccgtg gtcaaaacct
     3421 atgtaaaact caaaaaataa aacactagcc tactctcaaa gcactatact tcacagaaca
     3481 caggctctgc atctataagc gcctcataat ctttcaccga tcctccgttc ataaagcact
     3541 cgataatttt tttaccaaga gtgcataatt caggatcatt ataatattgt tgaagctcct
     3601 gagcaaaaaa atatgaaagg atttttgcac ccttatcata tgttccaata ccaatttcac
     3661 tctgaagctc cggcattaga agatactttt ctatctcctg accctcaata acaagacgat
     3721 taagagagta gccaagaaga gatgatctgg cttcaacaag ctcatgtggc atgaatttta
     3781 caccacctcg gcgggcaaga tattcacgca ttatccactg aggattaaaa ccgaccttcc
     3841 atgcaccgac atgctgatta ggacatagta catagtgcac ttttccagta ctttcgatct
     3901 gctttaataa cagatttgcc tgggttattt ttttacctgt tgcaaaaggc cagtaagagc
     3961 ctaccccttc agaactcatt gcgccagtat cgattatact cggatttgca tgtccacgag
     4021 gtgagaccaa tctccataac caggcaagag ccggcggtat aacatggaat atacccataa
     4081 taccttatgt aggatcgtca ctatggcaag gcggagtacg tactccaaaa cttcttatat
     4141 ttacacttac cggatgactg actatacccg gcacgacatc acgtgacacg acaaagcgcg
     4201 gatttggaca aggttttcca ggttcatctt cggtatgttc ccataccaga gcagtactgt
     4261 tggccggagc atccaggtta aggaatagaa gcggttttac cggatgtata gccaaagatt
     4321 caatatccgg atccgttccg tagttcttga tatggtcaac tcggataaac caaccagcct
     4381 cggcatctat tacccttaga gtgccgtcat ttttttgtat cgacggatgg cagactgcca
     4441 tatcatcggc aacaggctta agatcacatc cgcgtggcaa tacaagagac agctgttcat
     4501 tgctaagtac attttttgca agagtgattg ttccatcgta ctcgcgatgt atgtgttcgt
     4561 gcatttcgct ttttccgcca ccactagccc cttcgtgcat tatggttact ctattttcgt
     4621 atggcgttac tacctgcaca acagatgaat gtgtagttat ccagttttcc ttctcaccaa
     4681 agtcaatcaa agccgaatag actccttttt tggcacttgg tcccggataa agattataac
     4741 tgaatatctc atgagctttt tttgtccttt catgaacaac gacctgcttt ccatcgaaat
     4801 gggtatgtct aaaaggtgga gctgtataga ttgcgatttt aatatctgtt ccgtcatcaa
     4861 gggtgttcca gtcaacaatt ccttgaagca gacctagtcc aagacaaaaa aatcctgcat
     4921 ttacaggagc aatggcaata ccataatatc ccatatccat ttggcctgcc ttaaaaacaa
     4981 aagcggcgag ctcctgatct tgcatccatt taagagtttc gtctttcaaa agagcaaaat
     5041 cattacctgt tgattcttta tatgttggtt tatctgttga aaaagcgtct ccaattaaca
     5101 tgcttgcagg atcacgccta cgcatgtatg gatcaaaata attcacggat attccatttt
     5161 taactctaga tacaagtgcc tctttaatct caccttttcc cggaatttca taagaaacca
     5221 ttttctcgcc atttacttca tttagtatag acaattgata aagatcatct atattagaag
     5281 ccatacttac agatttgcat ccgtctaaaa cttccttggc aaaatccggt agtttcagtt
     5341 tttgaataat ctctttcatt taatcctcct tttttaaggc atctattttt tcatgctaca
     5401 tattcaatat tagtttcata agcaggttct atttttctgg gcacaaaaaa agccggcacc
     5461 tgagagctaa aactcattag taccggctta cccgacggct ccttttaaaa aggttccatc
     5521 aaatcttcca tatttttaaa actgctccgc agctttttta aagcttgaag acagtgctat
     5581 ttctgtaaat aattaagaaa ctaaatttca aattttccgt tgagtataaa tacgataatt
     5641 cgctcacctg tttttgtact aatatcggta tgaagactca ataccttctt ttgagtcaca
     5701 tctccaacca taacctgaag catggccttg gcactctcaa gtagctgcat tctgacctgt
     5761 ttgataaggc ccgcaccatc cggattttgc gccaacttct cttcagcagg agttaaaacc
     5821 cccttaagcc tgacaacaac catatcatct atgatggttg ttttggtttc tatgggacca
     5881 cgccccatat actccttttc gaagcggata actgtatccg aaatctgcgc ttctatttgc
     5941 cctttggtca tattaccaac cctttcggct gttagaaatg catattttaa atctccgcga
     6001 gaaacagata tttaaaatct tcactctttt ttaccataac attaacagtt ataaaattgt
     6061 caagtttttc atttttttat gaattttgat tttttatttt tttgttacat gaaaaaattt
     6121 atcgaattaa aaataaaaga ctaataaaca ggaaagtttt tgatttttac atttttttaa
     6181 ctaaattagt tctataagcc agatttgctc tatatcaaga aatacttgtt ttaaacggat
     6241 ggataagatg aaacacagga ttacattttt tattgtgtct gccttttttg taataatctt
     6301 tatcatatcg ttatcttgta ataaaaaaga ctcagaggcc aaaccaatta caatacggtg
     6361 gtggcatatc aattcagaca aacaatccca agttgtattt caaaatattg ctaatgaatt
     6421 tgagaaaacc aatcctgatg tgattattaa aattaccatg ttagataata tggaatataa
     6481 accaaaactg gaactggatt tcgctgcagg tgaccctcca gacatatttc atagttgggg
     6541 tggaggaggt cttgccgaac aagtcgaagc tggtcatctt cgagatatta cctcttatgt
     6601 aaaaagcgat aattggcaaa gcaaaataag cccgatagct ctggaaattt atagccataa
     6661 tggtaagatt tatggttttc ctcatgatct tggcgcagta ggcttctggt acaacaagga
     6721 gcttttaaaa aaagttgggt ataccagttt tccagagaca tggcatgact tttacaaatt
     6781 gtgcgaccgc cttaaaaaat cgggaatcac tcctatatct atcggtatag cggatagatg
     6841 gccggtaatg tactactggg tttacttagc aatgcgtatt ggaggtgaag atctgttttc
     6901 tgatattcac aaaggcaata tcaaatttaa taacccggat cttattaaag caggactgat
     6961 aatgcaagat ctgtttaaac acgggtattt tcaatcatca tctatcggtg atgactttat
     7021 tactcaatcg cgttatatgg gagatggcga atgtgcgatg caactcatgg gtcaatggg
//
LOCUS       DAOTGZ010000145         6921 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_3064
            NODE_13007_length_6921_cov_5.613021, whole genome shotgun sequence.
ACCESSION   DAOTGZ010000145 DAOTGZ010000000
VERSION     DAOTGZ010000145.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868088
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 6921)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6921)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:22:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,785
            CDSs (total)                      :: 2,751
            Genes (coding)                    :: 2,732
            CDSs (with protein)               :: 2,732
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 12 of 19
            Pseudo Genes (internal stop)      :: 5 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6921
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13007_length_6921_cov_5.613021"
                     /isolate="AMR_MDS_3064"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..571
                     /locus_tag="P5123_09570"
     CDS             <1..571
                     /locus_tag="P5123_09570"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRX16552.1"
                     /translation="YRKKEDKYIVDYSRVNDIQTERFLFLKQINQLHDMLIVLDTELL
                     GVVNPQMNRDKLPLATNEGQQNILNLFLNKNIPHSIRKKYVDYQKKVFGLPVGKSEQN
                     EEVNLAFYFETGSIDEDIFKQLLCVYDSMIGIESLCDIEVVLDKFRKGEFSYIQDTKS
                     FKYDLVDSLFMRHFYTNYDLKKLPLIVNG"
     gene            complement(554..2053)
                     /locus_tag="P5123_09575"
     CDS             complement(554..2053)
                     /locus_tag="P5123_09575"
                     /EC_number="7.5.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013254229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter ATP-binding protein"
                     /protein_id="HRX16553.1"
                     /translation="MNKKTILEIKNVNKSFKDEFALSNINLKLYSGDVHVIMGENGSG
                     KSLLMKIIAGFFTVDSGEILYNGAVINHPTVNKAIKSGIYYQHQDLPVFENLSAAENI
                     YFDYRISTKKMLKTWNALENISECTKLFKKLNINLSPIKNMSHYGFAERQLLFAAKAF
                     ISDAKVVIFDEPTAGMSEVERNIFFDILALIKQKAEAILYISHRLDEITKVGDVLSVM
                     EHGRLISTNDIRNATKQDIIRLITGKRKIHQFPKISIKPNSECVLSVKNMTSDSILKD
                     VSFNLHKQEILGITGLMGSGRSRLANCLFGIKQTNSGSIFVSGKKHLFMHPLDALLAG
                     ISLIPDDRQANGIFYPLNMRNNMTLPNLKRFLNNSILDDNILRLVVNDYVKKLGITPD
                     DSDNGIQFFSGGNQQKILLARCFMKRSSIYIMDEPTRGIDIASKIDIYNAMNNLIVKG
                     ASVILISSDIDEIIGMCDRILILADGRITAELLQKEATKEKIYSLAIND"
     gene            complement(2050..2838)
                     /locus_tag="P5123_09580"
     CDS             complement(2050..2838)
                     /locus_tag="P5123_09580"
                     /inference="COORDINATES: protein motif:HMM:NF012669.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR family DNA-binding transcription
                     regulator"
                     /protein_id="HRX16554.1"
                     /translation="MFAAERKRIIIKYLLENNKAEVVKISEMLNVSEVTIRRDFEKLE
                     NEGFLIRTHGGAILHNPSEKTYTKEPTVKAAEDEENNEIAETAMALVKENDVIMITSG
                     NISRLLAKKLSAKNGLTILTNDLIVALNASAGGQNRVICLGGEIDQDNVSVTGTITLR
                     NMQKYHVSRVFIEVDGISEKLELSVSSQQKAELIESAIESADHTTLLCNYTLFGTKAF
                     MKLGSVTMVNSLITNPILSDKYKAAIYDKNVVLYTSINAFEGNV"
     gene            3182..4252
                     /gene="chvE"
                     /locus_tag="P5123_09585"
     CDS             3182..4252
                     /gene="chvE"
                     /locus_tag="P5123_09585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013970188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter substrate-binding protein"
                     /protein_id="HRX16555.1"
                     /translation="MKRIVKFVTALITLALIVSVASCGKNSGKKIVGIAMPTQSSQRW
                     IQDGGNMKKILEDRGYQVDLQYAEDSIDAQVAQLENMITKGADCLVIASIDGETLTNT
                     LQKAADAKIPVIAYDRLIRNSEHVSYYATFDNFLVGVQQGGYIVDKLGLADGKGPFNI
                     EIFAGSPDDNNAYFFYNGAMSKLQPYIDSKMLVVKSGQIEFDKVATLRWDGATAQQRM
                     DNILTAHYTSSNVDAILSPYDGISIGILSSLKSVGYGSGSKKLPIVTGQDAELPSVKS
                     MIAGEQTQTVFKDTRTLAKRAADMVDSVLKGEDAEVNDTKTYDNGIKVVPSYLESPVS
                     VDINNYKEALIDTGYYTEDQLK"
     gene            4309..5853
                     /gene="gguA"
                     /locus_tag="P5123_09590"
     CDS             4309..5853
                     /gene="gguA"
                     /locus_tag="P5123_09590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013254226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter ATP-binding protein"
                     /protein_id="HRX16556.1"
                     /translation="MANIHLEMRGITKKFYGVTALDSVDLTVNNQEIHAIVGENGAGK
                     STLMKVLSGVHTHGSFDGEIYFNGQKCSFNNIKDSEKLGIVIIHQELALIPFLSIAEN
                     IFLGNERAVKGVINWNDSISEANKLLAKVGLNEKSSTLVDSIGVGKQQLVEIAKALSK
                     DVKLLILDEPTAALNDEESNKLLNLLVDLKKQGITSILISHKLNEIEKVADAITVIRD
                     GRVIDNLHKSKDIIDEDRIIRSMVGREITDRFPSRSSNIGEEVFRVENWTVYHPEHTD
                     TKAIDNVSFSVRKGEVVGFAGLMGAGRTEIAMSIFGKSWGVKHSGKIIKNNKEINFHN
                     VSDAISHGIAYSTEDRKTYGLILLDNIMRNLTLSSLNTISKNGIINSNEEVLTSQNYR
                     SKLNVRCSGLEQKVEDLSGGNQQKVVLGKWIMANPDLLILDEPTRGIDVGAKYEIYTM
                     INNLVAEGKSVIMISSEMPELLGMCDRIYVITEGRIAGELSAKEASQESIMKCIITDQ
                     RKRSLA"
     gene            5850..>6921
                     /gene="gguB"
                     /locus_tag="P5123_09595"
     CDS             5850..>6921
                     /gene="gguB"
                     /locus_tag="P5123_09595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020612414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HRX16557.1"
                     /translation="MTGLKSYFKNNIRQYGMIIALVFIMVLFQILTNGILLKPMNITN
                     LVLQNAYVLILAVGMLLAIITGNIDLSVGSIVALVGALSAVMMVTLKMPVVFAVGLSL
                     LIGLAAGSFQGLFIAYFKLPPFIVTLAGMLVFRGLTMVILKGQTLAPFPKSFQAIAAG
                     YLPGREKEAILGLTVAALTAIIIGILMSAIYILFELNSRKNKINYGFEVLPKSFEFFK
                     WSIIIITINAFTISLAAYNGIPIVLILLLVIIIIYTFISQKTIAGRHIYALGGNSKAA
                     KLSGVKTKRVMFWVYANMGVLSAVTGLIVAGRLNAATPKAGNMFELDAIAACFIGGAS
                     ASGGIGTVVGAVIGGLVMGVLNN"
BASE COUNT         2197 a         1160 c         1318 g         2246 t
ORIGIN      
        1 atatcgtaaa aaggaagata agtatattgt agactactcc agggttaacg atatccagac
       61 agagcgtttt ttatttttga agcagataaa ccagttgcac gatatgctta tagttttgga
      121 tacagaacta cttggtgttg ttaatccgca aatgaatcga gacaaactac ctttagcaac
      181 aaacgaaggg caacaaaata tacttaattt gtttttaaac aaaaatatac ctcatagtat
      241 tcgtaaaaaa tatgttgatt atcagaaaaa agtgtttggt ttgccagtag gaaaatccga
      301 gcaaaatgaa gaagtaaatc ttgctttcta ttttgaaaca ggatcaattg atgaggatat
      361 ttttaaacaa ctgctttgtg tttatgattc tatgattggt attgaaagtc tttgtgatat
      421 tgaggtagta ttagacaagt ttcgcaaggg agagttttca tatattcagg ataccaaatc
      481 ctttaagtat gatctggtgg attcgctttt tatgcgacat ttttatacaa attacgattt
      541 aaaaaaatta ccattaatcg ttaatggcta aagaataaat tttttctttg gtggcttctt
      601 tttgtaacag ttcggctgtt attcggccat cagctaaaat tagtattctg tcacacatac
      661 ctataatttc atcgatgtca gaagaaataa gtataactga agcgcctttc acaattaagt
      721 tattcattgc gttatagata tcgattttag atgcgatatc tattccgcgt gtcggttcat
      781 ccattatgta gattgaactt cgcttcataa aacatctggc taacagaatt ttctgctggt
      841 taccaccaga aaaaaattga atcccattat cagaatcatc aggtgtaatc cccagctttt
      901 ttacataatc gttaacaacc agcctgagaa tgttatcgtc caaaatgctg ttgtttaaaa
      961 accgtttaag atttggaagt gtcatgttgt ttcgcatgtt tagcggataa aaaataccgt
     1021 ttgcttgcct gtcatcgggt attagagaaa tacctgcgag caaagcgtct agagggtgca
     1081 taaatagatg ttttttccca gagacaaaaa tgctcccgct gtttgtttgt tttataccga
     1141 aaaggcaatt cgcaagacgg gatctgccag aacccatgag gccagtaata cccagaattt
     1201 cctgtttgtg aagattgaaa ctaacatctt taagaataga atcgcttgtc atgtttttga
     1261 cactcaaaac acactcgcta ttcggcttaa tagatatctt aggaaactga tgaatcttac
     1321 gttttccggt tatcaggcgt atgatatcct gtttggttgc atttctgata tcatttgtag
     1381 aaataagtct tccatgctcc attacagata atacatctcc gacttttgta atttcatcca
     1441 gtctgtggct aatatataaa attgcttctg ccttctgttt gataagtgca agaatatcaa
     1501 aaaatatatt tctctcaact tcgctcatgc cggcggtggg ttcatcaaaa attacaactt
     1561 ttgcatcgga tataaaggcc tttgccgcga aaagaagctg tctttcggca aaaccataat
     1621 ggctcatgtt ttttattggc gagagattta tgttaagctt tttaaaaagt ttggtgcatt
     1681 ctgaaatgtt ttctaacgca ttccaggttt ttaacatttt ttttgtactt attcggtaat
     1741 cgaaatagat attttcagca gcagataaat tttcaaaaac aggtagatct tgatgttgat
     1801 aataaattcc gctcttgata gccttgttaa ctgtgggatg attaataact gctccattgt
     1861 atagtatttc ccctgaatca actgtaaaaa aacctgcaat aatcttcata agcagagatt
     1921 tcccagatcc attctctccc ataatcacat gtacatcgcc agaataaagc ttaagattta
     1981 tatttgataa cgcaaattca tctttaaatg atttattgac atttttgatt tccagaattg
     2041 tttttttatt catacattac cttcgaacgc gttaatcgat gtataaagaa caacattctt
     2101 gtcgtatatc gcggctttgt atttatcaga aagaattgga ttagttataa ggctgtttac
     2161 cattgtcact gaaccaagct tcataaaggc ctttgttccg aaaagtgtat agttgcagag
     2221 aagcgttgta tgatcagcac tttcgattgc agactctatg agttccgctt tttgttgtga
     2281 ggagactgac agttcaagtt tttcactaat accatcaact tctataaaaa ctctgctcac
     2341 atgatatttt tgcatgtttc taagagtaat ggttccagtc accgaaacgt tatcttgatc
     2401 gatctctcca ccaagacaaa taaccctgtt ttgcccacca gcggatgcgt ttagggctac
     2461 gatcaaatca tttgttagga tcgttaatcc gtttttcgcg gataattttt ttgccaataa
     2521 tctgctgatg tttccactag taatcattat tacatcgttt tctttaacca gtgccatcgc
     2581 agtttcggca atttcattgt tttcttcatc ttcggcggct tttactgtcg gctctttggt
     2641 gtatgttttt tcagaaggat tatgtagaat tgcaccaccg tgtgttctta tcagaaatcc
     2701 ctcgttttcg agtttttcaa aatcacggcg tatagttact tcggaaacat ttagcatctc
     2761 actaattttc actacttctg ctttgttgtt ttccaaaaga tatttaatta ttattctttt
     2821 tctttctgct gcgaacattt tttacctgag cttttttggg ttattattag gtaatttggt
     2881 catcattata acagtattgt ttatatctca acttaaaatc gcattataca ataagagcgg
     2941 ctgatgaaca aaacgaaaca aaatgatcat tatttttaat caattttacc taattttaat
     3001 aaaaaatgaa taaaacattc aaaaaaagac aagaaatatt ttttaaattt gacaaatata
     3061 acaatatgac ttacgaaatg atcaaatatg aagcaatgcg taacccactg tccgtctcta
     3121 cataactgta ttgcctattt aaaaatccgt ctatcggaaa ataaaaaaag ggaggtttaa
     3181 aatgaaacgt atcgtcaaat ttgtcactgc tttaataact ttagcactta tcgtttctgt
     3241 agcatcatgc ggtaaaaaca gcggaaaaaa aattgtaggt attgcaatgc cgacacagtc
     3301 atctcaaaga tggattcaag atggcggaaa catgaaaaaa attctcgaag acagaggata
     3361 tcaggttgat ttacagtatg ctgaagatag tatcgatgct caggttgctc aacttgaaaa
     3421 catgattact aaaggtgcgg attgtctggt tatcgcttca attgatggtg aaacattgac
     3481 aaacacactt caaaaagccg ctgatgcaaa aattcctgtt attgcatacg atcgtctaat
     3541 acgaaatagt gagcatgtaa gctactatgc aacctttgac aacttccttg taggagttca
     3601 gcagggtgga tatatcgttg ataaacttgg tcttgcagat ggtaaagggc cttttaatat
     3661 cgagattttt gctggttcac cggatgataa taatgcttat ttcttttata atggagcaat
     3721 gtcaaagctt cagccatata tcgatagtaa aatgcttgtt gtaaaaagtg gacagattga
     3781 attcgataaa gttgcaactt tgagatggga tggtgcaaca gctcagcaaa gaatggacaa
     3841 catacttact gcacattaca catcatcaaa tgttgatgca attctttctc catatgacgg
     3901 catctctatt ggtatacttt cttctcttaa aagtgttgga tacggttcag gtagcaaaaa
     3961 acttcctata gttacaggtc aggacgcaga acttccttca gttaaatcca tgatcgctgg
     4021 tgagcaaaca caaactgtat ttaaagatac acgaactcta gcaaaaagag ctgccgatat
     4081 ggttgactca gtgcttaaag gtgaagacgc agaagttaat gatactaaaa catatgataa
     4141 cggtattaaa gttgttccat catatcttga atctccagtt tctgtagata tcaataacta
     4201 taaagaagct cttattgata caggttacta tacagaagat cagcttaaat aaatattagc
     4261 atgaagggaa cctataaacg ggttctcttc atgtttaagg atatcaaaat ggcaaatata
     4321 catttggaaa tgcgcggtat aacaaaaaag ttttatggtg ttacagcgct tgactctgtg
     4381 gatcttacag ttaataatca ggagattcat gccattgtcg gtgaaaacgg tgccggtaaa
     4441 tctacgttaa tgaaagtatt aagcggagtt catactcatg gatcatttga cggtgagatt
     4501 tattttaatg ggcaaaaatg tagttttaac aatatcaagg atagtgaaaa acttggcatt
     4561 gtaattattc atcaagagct ggctcttatc ccctttttat caatagccga aaatatattt
     4621 cttggaaacg agagggcagt aaaaggtgta ataaattgga atgactctat ttcggaagca
     4681 aataaacttc ttgcgaaagt tggcttaaat gaaaaatcct caacattggt agacagtatc
     4741 ggagtaggca aacaacaact tgtagaaatt gcaaaagctc tatcaaagga tgttaaactt
     4801 cttattcttg atgaacctac tgcggcgtta aatgacgaag aaagtaataa gttgcttaat
     4861 ttacttgtag atttaaaaaa acagggtatt acatcaattc ttatctcgca taaacttaat
     4921 gaaatagaaa aagtagcaga tgctattaca gtaatccgtg acggaagagt catcgataat
     4981 ctgcataaat caaaagatat aatagatgaa gatcgtataa ttcgaagcat ggttggtcgt
     5041 gaaattaccg atcggtttcc atctcgaagc tcaaatattg gagaagaagt ttttcgagtt
     5101 gaaaattgga cggtatatca tcccgaacat accgatacaa aagctataga caacgttagt
     5161 ttttccgtta gaaaagggga ggttgttggt tttgcaggtt tgatgggagc aggtagaacc
     5221 gaaatagcaa tgagtatctt tggtaagtct tggggagtta aacactctgg aaagataata
     5281 aaaaacaaca aagaaattaa ttttcataat gtcagtgatg caatttctca cggaattgcc
     5341 tattcaactg aagatagaaa aacctatggg cttatacttc tcgataatat aatgagaaat
     5401 cttactcttt ccagcctcaa tacaatcagt aaaaatggta tcatcaatag caatgaggaa
     5461 gtcttgactt cgcaaaacta tcgtagcaaa ttaaacgtta gatgctctgg acttgaacag
     5521 aaggttgaag atctttcggg tggtaatcag caaaaggttg tattgggtaa gtggattatg
     5581 gcaaatcctg atctcttgat tcttgatgaa ccaacaagag gtattgatgt cggagctaaa
     5641 tatgaaattt acacaatgat aaataatctt gtggctgaag gaaaatcggt tattatgatc
     5701 tcatcagaaa tgccagaact acttggaatg tgtgaccgga tttatgtgat cactgaaggg
     5761 cgaatcgctg gagagctatc ggcgaaagag gcttctcagg aaagcataat gaaatgtatt
     5821 ataacagatc aaagaaaacg gagtttagca tgacaggatt aaagagttat tttaaaaaca
     5881 acattcgtca atatggaatg ataatcgcac ttgtattcat catggttctg tttcaaatcc
     5941 ttacaaacgg tattttgctt aaacccatga atattaccaa tttagttttg caaaatgcct
     6001 acgtattgat tcttgctgtg ggtatgctgc ttgcaatcat taccggtaac atagacctct
     6061 cggtaggttc gatagtcgcc ttagttggcg ccttgtcggc tgtaatgatg gttaccctaa
     6121 aaatgccggt agtttttgct gttgggcttt ctcttctaat agggcttgcg gcagggtcat
     6181 ttcaagggct ctttattgca tactttaaac tacctccatt tattgtgacc ttggccggca
     6241 tgcttgtttt tagaggcctc acaatggtaa tacttaaagg tcagactctt gctcctttcc
     6301 caaaatcatt ccaggctatt gcagcgggat atcttcctgg cagagaaaaa gaagccattc
     6361 tcgggttaac agttgctgcg cttacagcaa ttataattgg aatattaatg tcagcaatct
     6421 acatcctgtt tgaactaaat tccagaaaaa ataaaattaa ttatggtttt gaagttttac
     6481 ctaaaagttt tgaatttttt aagtggagca tcatcattat aacaattaat gcgttcacaa
     6541 taagtcttgc ggcatataac ggaataccaa ttgtgcttat attattgttg gtaatcatta
     6601 ttatatatac ttttatatca caaaaaacga tagcggggcg acacatttac gcgctaggtg
     6661 gaaactcaaa agctgcaaaa ttatcgggtg ttaaaacaaa acgtgttatg ttctgggttt
     6721 atgctaacat gggtgtattg tctgcagtta ccggactcat tgtcgctggt cgtttaaacg
     6781 ctgcgactcc gaaagccggt aacatgtttg aactggatgc aatcgcggct tgctttattg
     6841 gtggtgcatc agcatccggt ggtataggta ccgttgttgg agcggttata ggtgggcttg
     6901 taatgggcgt tttaaataat g
//
LOCUS       DAOTGZ010000146         6908 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_3064
            NODE_13036_length_6908_cov_6.521961, whole genome shotgun sequence.
ACCESSION   DAOTGZ010000146 DAOTGZ010000000
VERSION     DAOTGZ010000146.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868088
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 6908)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6908)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:22:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,785
            CDSs (total)                      :: 2,751
            Genes (coding)                    :: 2,732
            CDSs (with protein)               :: 2,732
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 12 of 19
            Pseudo Genes (internal stop)      :: 5 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6908
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13036_length_6908_cov_6.521961"
                     /isolate="AMR_MDS_3064"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(201..956)
                     /locus_tag="P5123_09600"
     CDS             complement(201..956)
                     /locus_tag="P5123_09600"
                     /inference="COORDINATES: protein motif:HMM:NF016668.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ChbG/HpnK family deacetylase"
                     /protein_id="HRX16558.1"
                     /translation="MKKELILNVDDLGLHIDTVLAVQELWENKRISTVSLLSTSSILD
                     ATIDKLKKIGIPVGVHLILDGDRPILEKNKIPSLIDEQGFMLNDYNAIRKRVKPDEVF
                     NEFSAQIENIQKRGVSVSHLDSHRGFCFLIPKLRKVYRELGEKYNLPLALPDNFIFNK
                     TRRLVPGSTDSLNGIYDLEEETFENRIKAYDLMLSRLGYGKHYCFSHPSPPTKSIMEN
                     LPDFKIRNNDYALFLSDEWSNLLKKYNITLSSF"
     gene            1379..2131
                     /locus_tag="P5123_09605"
     CDS             1379..2131
                     /locus_tag="P5123_09605"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRX16559.1"
                     /translation="MKGKRLIAKSIFMTHVRRIGIIRTAFGGGLMYVSILEFIFLHLT
                     TIVVLYNWILAPALKLKRYKIRNFIVLDRNRIDRMRMFDRINCLFCGYANGTAHLWNI
                     QLDELASASAKKGNIFAKFIGILYSMLLAVFLICNFIFSKLLFLVISAFLGLHWAKTS
                     EIRNKLNEENYAGNFPFLFKKLIRFAKIYAKSLATNLEQIESAWCPLTHMKREEASYP
                     EHHKNFYQWDKLDDVIEVLAKEGTVSERKPKY"
     gene            complement(2141..2731)
                     /locus_tag="P5123_09610"
     CDS             complement(2141..2731)
                     /locus_tag="P5123_09610"
                     /inference="COORDINATES: protein motif:HMM:NF012515.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PfkB family carbohydrate kinase"
                     /protein_id="HRX16560.1"
                     /translation="MYRIPGITASNEIINDEKGERFGVEGAWQNGVYYDFLLSEHDWA
                     YIEEFEIWSTHANGQNYSEALKRKRSNNFLAVDFLDMDTYELLEKGLSSIDIAYFGGT
                     PNQLDELVYISKSFKGIIVLTLGSEGSIAINKGETCIQPALPLNRVIDTTGCGDAFQS
                     AFAGNYLLTGDIKSSLLRGAEAGKTAAMKYGAVCLE"
     gene            complement(2743..2943)
                     /locus_tag="P5123_09615"
     CDS             complement(2743..2943)
                     /locus_tag="P5123_09615"
                     /inference="COORDINATES: protein motif:HMM:NF012515.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PfkB family carbohydrate kinase"
                     /protein_id="HRX16561.1"
                     /translation="MNNIKTACFSVAAMDYFPQQKEYSAGGNSLNQAIGFRNLGFKSS
                     FIGAIGTDKAGDQIEQLLIKKM"
     gene            3256..4680
                     /locus_tag="P5123_09620"
     CDS             3256..4680
                     /locus_tag="P5123_09620"
                     /inference="COORDINATES: protein motif:HMM:NF028468.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MASE3 domain-containing protein"
                     /protein_id="HRX16562.1"
                     /translation="MKLRSSKDDALLLVPVVVFVVLSYFVSGANFLFFHIIIEFVVIL
                     TAFGISVLTLTAGDKVESGFLRFIGSAFLWIGILELFHTLTYRGMNIFVGLDSNHPTQ
                     FWIAARWMEVITFILALLWRKSGVVYWRTSLVYALVTTFVIYVIMMTSWFPDCYIEGE
                     GLTNFKKISEYLISGIFLLLMLHFMFIKKDFSNDIRRQMVLALLFAGLAEIFFTFYIN
                     VYGISNYIGHVLKIISVVIIFRAIVHKGIREPQDILYYDLLQKEEALKAALEEKEVLM
                     REIHHRVKNSLALVASFIAFESKDDNGSECGKKLNSLKERIKSVLLIHEQLSSTGEVG
                     QLDFSVYVKDLVDNLFLSADTGEGRIKWKVDIPQIIMSAKKAMSLGIIINEIVTNSIK
                     YAFPDNRNGTILITMKRLTDKYELIIEDDGVGSTRAKKAGSSGLGKMIIASLVEQLEG
                     TLNVLPLTDVDKVSGTGYLITIPV"
     gene            4775..4990
                     /locus_tag="P5123_09625"
     CDS             4775..4990
                     /locus_tag="P5123_09625"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRX16563.1"
                     /translation="MSMQTKTEVKNEGIVAIAALKAKVYYWTRVSEGLCIKSAGYTDD
                     SLLEIEKYMSLISKLFVVSLVLTLCMF"
     gene            complement(5128..5787)
                     /locus_tag="P5123_09630"
     CDS             complement(5128..5787)
                     /locus_tag="P5123_09630"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRX16564.1"
                     /translation="MEKNYFICPIHAVICIIILFFFSSIYSKEAATLSKRGNMDIETV
                     IRKGKEYIIKTALSVYEDKYKDMPINLSLYSITIWRNSTEIKVVFKRDVTYRPLHKKN
                     DNLYYDIVIEIFDEKVSPFDMFGTDKFFIASEEESVIIDIIKKKMKMPLPFCKNSIYE
                     TEEWYVASITSEHSFGKFFFDKNTLEEKRDMKIQGSYAPRIRPDTPEDPDPWIELFKR
                     K"
     gene            complement(5839..6282)
                     /locus_tag="P5123_09635"
     CDS             complement(5839..6282)
                     /locus_tag="P5123_09635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013071612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4844 domain-containing protein"
                     /protein_id="HRX16565.1"
                     /translation="MKKITTTLLGLTLLLFAACWQGQMKTPSNTMIKFEEFKNKEKFL
                     KDDRLFYPGISDPKLKPVLTEKINLVADDFIKLTDKPNSTDKDYQNAIKIGLERFSDI
                     YLQLDTEDRERVCNYFEELMDIVGLKSSGGHLNDFMYGFDPKKSK"
     gene            complement(6419..6826)
                     /locus_tag="P5123_09640"
     CDS             complement(6419..6826)
                     /locus_tag="P5123_09640"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRX16566.1"
                     /translation="MIRTLFAAVLVFGIFSYICASNQSIEPLIGHWFVAEKNYNYSSE
                     QNVNYEEIVTISFVFNKDNTGLWMIESIKKHTNGKSETNEEKIIYKWKISNKKIVLDF
                     YEMGRKFESDFDISDSILTLSDPEGKTRKYSKK"
BASE COUNT         2030 a         1204 c         1293 g         2381 t
ORIGIN      
        1 tacgtcaacg atgagcctgt tggtatgggc gaagatgatt ctctttgcga tatcggcact
       61 tttcactttg gggtttttcc ttcggatgac aagaatgaga caagtgtcag tcttgataat
      121 ataaagattt ggaacataga aggttggtaa taatcaataa aatttttaga caatcagtcg
      181 tcttcaggat gattgattgt ctaaaaagat gaaagtgtaa tattgtactt ctttagcaga
      241 ttactccatt catccgagag aaaaagggca taatcgttat tacggatttt gaaatcgggc
      301 agattttcca ttatggattt tgttggcgga gacgggtgag aaaaacagta gtgtttccca
      361 tagccaagtc tactgagcat aaggtcgtac gctttaatcc ggttctcaaa agtttcttcc
      421 tcaagatcat agatgccgtt cagtgagtca gtagaaccgg gcacaagcct tcttgtttta
      481 ttaaaaatga aattatccgg aagcgccaag ggaagattgt atttttcgcc aagttcacgg
      541 taaactttac gcagtttggg tattaaaaag cagaatcctc tgtgtgaatc aagatggctg
      601 acgctcacgc ctcttttttg aatgttttcg atttgagctg aaaattcgtt aaatacttca
      661 tccggtttta ctctttttct tatcgcgtta taatcattca gcataaaccc ctgctcatca
      721 ataaggctgg gtattttatt tttttcaagt atcggcctgt caccgtccag aatcaggtga
      781 acaccaaccg ggattccgat cttcttcagc ttgtcaattg ttgcatccag aatagatgat
      841 gttgataata agcttactgt agatatacgt ttgttttccc agagttcctg aactgccaaa
      901 acagtatcaa tatgtaaacc cagatcatcc acgtttaaaa tgagttcttt tttcatgatt
      961 ccaggctccg aaagcatatt ttgaaattga ttgtgaatct gaaggctgat tgatatttta
     1021 aaataatgtt tattggttat gaactgttta tattatttcc atattctatt taattatccg
     1081 ggactcatct ttgtcaatag agtttagcat tattttaagt tgatttatgt tttttgtcaa
     1141 gattgttgtt cctatttttt ataatttcag atgattcaaa tacagctaac gttaaacgtg
     1201 ttgaaagttt tcctgaaatt taaaaaaaag gcaattttag aaatttctcc tggagatatt
     1261 ttcagataat cgtggtagaa taatgttgga aaattttttg tgtacaaaaa caatccggat
     1321 aacctatatg gttaagatat agtaaacata tatatcacaa atggataata aaataaacat
     1381 gaaaggaaaa agacttattg caaaatcaat ttttatgaca catgtccgcc gtattggcat
     1441 aattcgtacc gcgtttggcg gcggtctgat gtatgtcagc atactggaat ttatttttct
     1501 gcatcttaca acaattgtag ttctttacaa ctggatcctt gctccggctt tgaagctcaa
     1561 aagatacaaa ataagaaact ttatcgttct ggacagaaac agaattgacc ggatgagaat
     1621 gtttgaccgg ataaactgtc tgttttgcgg ttacgccaat ggaacggccc atttgtggaa
     1681 tatccagctt gacgagcttg cctcggcgtc agcaaaaaaa ggaaatattt tcgcgaaatt
     1741 tatcggtata ctctattcaa tgcttcttgc ggtatttctg atttgtaatt ttattttcag
     1801 caagctgttg tttcttgtta tctcggcttt tctggggctg cattgggcaa aaacatccga
     1861 aattcgaaat aagctgaatg aagaaaacta tgcaggtaat ttcccctttc tgtttaaaaa
     1921 actgatacgc tttgcaaaga tatacgcaaa atctcttgcg actaatcttg aacagatcga
     1981 atcggcctgg tgcccactga ctcatatgaa gcgggaggag gcctcttatc ctgaacacca
     2041 caagaatttt taccagtggg ataagcttga tgatgtaata gaggttcttg ccaaagaggg
     2101 tacagtttcc gaacggaaac cgaaatactg attcactata ttattccagg cagacagctc
     2161 catacttcat ggcagctgtt ttgcctgctt cggcaccccg taacagagaa ctttttatat
     2221 ccccggtaag cagatagttt ccggcaaatg cactctggaa agcatcgccg catcctgttg
     2281 tgtcaatgac tctgtttaga ggtaaagcag gctggatgca ggtttctcct ttattgatcg
     2341 caatacttcc ttctgatccc aatgtcaaaa ctataatgcc tttgaagctt tttgagatat
     2401 aaaccaattc atcaagctgg ttcggagtgc ctccaaagta tgcaatatct atggagctta
     2461 aacctttttc cagtaattcg tatgtatcca tatctaaaaa atctactgct aaaaaattgt
     2521 tgcttctctt gcgcttaagc gcttcagaat aattttgtcc attagcgtgt gtggaccata
     2581 tttcaaattc ttctatatat gcccagtcat gctctgataa aagaaaatcg taatatacac
     2641 cgttttgcca tgccccttcg acgccgaatc gttcaccttt ctcatcgttg attatttcat
     2701 tacttgcggt gattccaggt attcgataca agtgagatat atctacattt tttttatcaa
     2761 taattgttca atttgatcgc ctgctttatc tgtgcctatc gcaccaataa atgaactttt
     2821 aaaaccaaga tttctgaatc cgattgcctg atttaaagaa tttcctccag cggaatattc
     2881 cttttgctgc ggaaaataat ccatcgctgc tacactgaag caggctgttt taatattgtt
     2941 caatatttct ttcccctctg taaacggttt tactatttta attatcctga ttttttcaat
     3001 ttattgcaga taatattatc cggttgctca attttaaact ttgaacagat caaactgtca
     3061 agactaatag cgttttgggc tttgtaaaag ttattcgcgc aataaaacaa ttcgggccga
     3121 tcctctgctt ttcttgaccg ggcgtacact attaggtaga ttaactttaa ccgtctgaaa
     3181 gcatctaaga agggtttggc ggcaagctgt tggtttgtta tcagattatc tttaaaatca
     3241 gcatggatgg cacaaatgaa attaagatct tcaaaagatg acgcgcttct tttagtgcct
     3301 gttgtggtat ttgtcgtctt gtcgtacttt gtttcggggg ccaattttct tttttttcat
     3361 atcattattg agtttgtagt aatacttaca gctttcggca tctctgtgct tacccttaca
     3421 gccggagaca aggtagaatc aggattttta cgatttattg gttcggcctt tctttggatc
     3481 ggcattcttg aattgtttca tacgctgact taccgtggaa tgaatatttt tgttggtctg
     3541 gattcgaacc atccaacaca gttctggata gctgcacgat ggatggaagt tattacattt
     3601 attctggcac tattatggag aaagtcaggt gtagtttact ggcgaacttc tcttgtgtat
     3661 gctctggtta caacttttgt tatctatgtt atcatgatga cctcatggtt tcctgactgc
     3721 tatatcgagg gagaagggct tacaaacttc aaaaagataa gcgagtatct gatatcggga
     3781 atatttcttt tgctgatgct gcattttatg tttatcaaaa aagatttttc taatgatatt
     3841 cggcggcaaa tggtgctggc tctgttgttc gcgggtttgg cagagatctt ctttaccttt
     3901 tatataaatg tttacggaat ttccaactat attgggcatg ttctgaaaat catatcggtt
     3961 gtaataatct tcagggcaat tgttcacaaa ggtatccgcg agccccagga tattctttat
     4021 tacgatcttt tacagaaaga agaggcgctg aaggctgcac tggaagagaa agaagtacta
     4081 atgagagaga tacatcatcg tgtgaagaac agtcttgcgt tggttgcctc ttttattgcg
     4141 ttcgagagta aggatgataa tggaagtgaa tgtggcaaaa aactgaactc cttaaaagaa
     4201 aggataaaga gtgttttgtt gattcatgag caactttcat ccacaggtga ggtgggtcaa
     4261 ctcgattttt cggtatatgt taaagatctt gtcgataatc tttttttatc tgccgatacg
     4321 ggagaaggca gaataaaatg gaaagtagat atacctcaaa ttatcatgtc cgcaaaaaaa
     4381 gcgatgtctc tgggaataat cattaacgag attgtaacaa acagtataaa atatgctttt
     4441 ccggataatc gaaacggaac gatcctcatt acaatgaaac gtctaacaga taaatatgaa
     4501 ctgataatcg aagacgatgg agtaggttcg acaagagcaa aaaaagctgg ctcaagtggt
     4561 cttggtaaaa tgattattgc ttctctggtt gaacagctgg aagggacgct taatgtgtta
     4621 cctttaacgg atgtcgacaa ggtttctggc acagggtatc ttattacgat acctgtgtga
     4681 agtaataaag gcattgtaat tttttgaaca aataatatat tcgcgccggt tagtagagta
     4741 agtgttaact attttgttat tgtgcagttt cgcaatgtct atgcagacta aaacagaggt
     4801 taaaaatgaa ggaatcgttg cgatcgctgc actcaaagca aaggtatact attggacacg
     4861 cgtctctgag gggttgtgca taaagtctgc tggatacacc gatgactcac ttcttgaaat
     4921 agaaaaatat atgagtttaa tttcaaagct ttttgtggta tcgcttgtac tgacgttatg
     4981 tatgttttaa tcgtgtgatg gttcggataa taaaagcagt aaaaacagtg acagctctaa
     5041 tctaatatac gaaaatgatt ataaaattgg caaaactgct gcgtctgtag gttttaagga
     5101 ataacttaat tgcgtattcg ggttgagcta tttgcgctta aacagctcaa tccagggatc
     5161 cgggtcttcg ggtgtgtccg gcctgatcct cggagcatag cttccttgta ttttcatatc
     5221 tcttttttct tcaagagtat tcttgtcaaa aaagaatttt ccgaatgagt gctcggaagt
     5281 aatactggca acataccact cttcagtttc gtaaatgctg tttttgcaaa aagggagggg
     5341 cattttcatt tttttcttaa taatatcaat tattacagac tcttcttctg acgcaataaa
     5401 gaatttatct gttccaaaca tgtcaaaagg agagactttt tcatcaaaga tttcaatcac
     5461 tatatcgtaa tagagattgt cgtttttttt atgtaaagga cggtatgtaa catctctttt
     5521 aaaaacaact tttatttccg tggagtttct ccatatggta atactatata gcgaaaggtt
     5581 aatgggcata tctttatatt tgtcttcgta aacggaaaga gcagttttga taatatattc
     5641 ctttcctttt cgaattacag tttcgatgtc catgtttccc ctttttgaaa gcgttgcagc
     5701 ttctttagaa tatatactgg aaaagaaaaa tagaataata atgcatataa ctgcatgtat
     5761 tgggcaaata aaatagttct tttccacact gtcctcttat aaaaattaaa aaaacaattc
     5821 ccaatttaca ttctttcatt acttgctttt ttttgggtca aaaccataca taaaatcatt
     5881 caagtgtccg cctgaacttt taagtcctac aatatccata agttcttcaa agtaattaca
     5941 aaccctttct ctgtcctctg tgtcaagttg caagtaaatg tcagaaaatc tttcaagtcc
     6001 aattttaata gcattttgat aatctttgtc ggttgaattc ggtttatctg taagttttat
     6061 aaagtcgtct gcaacaagat taattttttc tgttaaaaca ggtttcagtt tagggtcgct
     6121 aattcctgga taaaacagtc tgtcgtcttt taaaaatttt tctttgtttt tgaactcttc
     6181 aaattttatc attgtattgc ttggtgtttt catttgtcct tgccaacagg cagcgaaaag
     6241 aagcaaggtc aatcctagta atgtggttgt tatttttttc attgtcgtat ttcaaattcc
     6301 gtataatatt tatcgcaaca aaatctgtac gtccgtttta gcggttggta tctgtttatg
     6361 gtcgaaccct atatgtctgt acgttgaagc atgtaattat tagcagtaca gttcaaatct
     6421 actttttaga atactttctt gtttttcctt caggatcaga taaagttaaa atagaatctg
     6481 aaatatcaaa atcactttca aactttcgac ccatttcata aaaatccaag actatttttt
     6541 tatttgaaat cttccattta taaatgattt tttcttcatt tgtttccgac tttccgttgg
     6601 tgtgcttttt tatactttca atcatccata atccggtatt gtctttgtta aagacaaaac
     6661 taattgtaac aatctcttca taatttacat tttgctcact agaataattg taattttttt
     6721 cagcaacaaa ccaatgtcca atcaaaggtt cgatactctg attgcttgca cagatataac
     6781 tgaaaattcc aaatactaga acggcagcaa ataatgttct tatcatttta atatttcctt
     6841 tgatttaatt aaataatact ttcaatatgc ttgatacgaa cggtcagtat aaaaataaca
     6901 gccgacag
//
LOCUS       DAOTGZ010000147         6895 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_3064
            NODE_13073_length_6895_cov_4.680848, whole genome shotgun sequence.
ACCESSION   DAOTGZ010000147 DAOTGZ010000000
VERSION     DAOTGZ010000147.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868088
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 6895)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6895)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:22:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,785
            CDSs (total)                      :: 2,751
            Genes (coding)                    :: 2,732
            CDSs (with protein)               :: 2,732
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 12 of 19
            Pseudo Genes (internal stop)      :: 5 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6895
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13073_length_6895_cov_4.680848"
                     /isolate="AMR_MDS_3064"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..2746
                     /locus_tag="P5123_09645"
     CDS             <1..2746
                     /locus_tag="P5123_09645"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRX16567.1"
                     /translation="KPSADLATCPSQLIVEVSADKRIWSAVIDQRHFFPDSDKLFLWN
                     FNSIPARYLRFEISPIELKKGNFFISIHDIEIHALPEGLNHSHTFSEGPSYASVFQPG
                     LVRFAKDGEDSRDAAIRASDRRLRDATTFFKGIVQFAEDGQATPNTAILASDSRLKPA
                     TELSAGIVQLAYDYEIKPGTVVQASDPRLKDASEKSSGIVKLCPDGAYSELGVVRGND
                     SRLKNATESAFGIVRLAKNGESEGSSVVQANDSRLRDASEISKGIVAFSADGEEIEGK
                     AVQANDRRLKNATTKSKGIVELAEDGEDSPLTAVQGNDKRLKNASTESRGIVELAENN
                     EIKAGAVVQSNDDRLRDASTSYKGIVRLAEDGEESPLAAVQGNDKRLKSATTVAEGIV
                     ELAEDGEVAESKVVQSNDKRLRDATTTYKGIVEMAEDGEVAAERVVQSDDRRLKDATT
                     KTTGIVQFSENGESSPLKAVQSNDDRLRKSSEDYPGIVQLAKNGENSPFKCVQSNDHR
                     LRDGSEEQKGIVQFAHNGEISPLKAVQSDDQRIQPAGTVTAGIVRLAKDGESTEGTVV
                     QSNDSRLHDGSEDEKGIVRFAKHGESRAQFAVQSSDPRLNDAREPLNHEHNYAQKNHN
                     YNEHSGTLSISGEKAEVFGGLTPPSDNSVIIYGKNSSSEAGAIGVSGVALPSKSKKNE
                     FSGYGVVGHSDFVGIRGQATGHEKNPRGAGVLGISRNAPGGVFISEHDYSLLVDGYAS
                     IDDIDPSLKMVGNGKALQVKGESHFHGVIHIDNDKDNDATTNIVEMFELENDDFISHG
                     DVVIASEKGASLLKRTHKAYDRTVIGIIAGKPALSINNSGKETKLYPIALAGKVMCKV
                     DARTQPIAPGDLLVTSDTPGCAMKGTIDSFEKVGSVIGKALDSLESGTGVIAVFLTHQ
                     "
     gene            complement(2797..3468)
                     /locus_tag="P5123_09650"
     CDS             complement(2797..3468)
                     /locus_tag="P5123_09650"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRX16568.1"
                     /translation="MKKIIILTVVLSITGILLGQSEEEPDKATVVATEPGGARWGSSF
                     DEVREVARGKLQFYRKDHYIITKDGEITYTYGFFYEDPEMLGMDPEEEMEPEPATAGA
                     ATEGATTEGATTEGATDDEKTGEAQYLYGITSFPYLAMEDVKKKLIEKYGEPTAESIV
                     RNSGVIIWDLETTQISAWIDAYEKKPYCRKIVYVSKEVSKKLKDYNYKIFNRKEIDTL
                     NRIKP"
     gene            3864..4169
                     /locus_tag="P5123_09655"
     CDS             3864..4169
                     /locus_tag="P5123_09655"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRX16569.1"
                     /translation="MKAVLQEILSAEKEAEKIVRDARDKASKMIRETDAKINEEKIKL
                     KDNIRKSLQKRIIAAAENVYQENENKSIGNHDLPNNSYELAINEISAIICNTSVKEE"
     gene            4174..5307
                     /locus_tag="P5123_09660"
     CDS             4174..5307
                     /locus_tag="P5123_09660"
                     /inference="COORDINATES: protein motif:HMM:NF014094.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATPase subunit"
                     /protein_id="HRX16570.1"
                     /translation="MGGLSDYAYINSRIHALMAKLLAPDFFEKLLKSASLEEALYMFQ
                     STDFNFIPKTYSRTGDLKIVEKELITFLYQDVIKLQKHSPAILIPLVKALSERSRYSA
                     FKTALRLWFDQSFRKRSISDYTEYIPRDIPLEGVSYDSIINASNTESLTEHLRTTPYA
                     CVIQELEKISESGNLLRCEIILENSYYKYLYNTVISYSLSTCSTLFAKIHFYNNFANM
                     QYSSSCLCNSLNLFHSDKNTDLKLLLEQDIDMINLGRLVRLSNIVSPESQNILYIPGG
                     NRIIESDFINDNRPSTAEILNRFSLSSMTDKLTKLEQHELHAYIKQSIQQKTINFYTR
                     VKTRSLFDESLAFVYIRLRENEISRIISTLNTLHYTANIHGGN"
     gene            5307..>6895
                     /locus_tag="P5123_09665"
     CDS             5307..>6895
                     /locus_tag="P5123_09665"
                     /inference="COORDINATES: protein motif:HMM:NF013647.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0033179 - proton-transporting V-type
                     ATPase, V0 domain [Evidence IEA];
                     GO_function: GO:0015078 - proton transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATPase 116kDa subunit family protein"
                     /protein_id="HRX16571.1"
                     /translation="MIFPQSMIRLNAIILRKDTDSVTKALLNEGIIDFTDIHDKTISF
                     SNQLSPFDKDSAETDIIEIRSRIEQLLSQVNIPLPDTSDLNAEKLVPVDPKEVTDRLD
                     ELNLQIRQIRDKQKDIQQSINRKTEIMRQTDLFVSIEASPPPESSVRVATGLIRKSRK
                     EGLTQALSLYPAVIHFSQSTQDEISAIIIYMSRDEERIGTALDRYQWQSVEFPPELTG
                     KKEEILHKLASSIEELNHEQIGFEKEAEDIIRKFAPILTESWKNLTITAQCIKIQNSF
                     GKTSDTVIFSGWLPCHLKNAAEKIIKKITNGKYYIQWVKPEDDPLLNSSIVPVKLNHG
                     TFLKPFQWVVENYSMPQYGSIDPTVIVAFSFLTMFGLMFGDAGHGLVLALCGAIAAFI
                     KRKKHDTAFELARLIMYCGLSSVVAGALFGSYFGLGIFKPLWFDYHGIVLGHKGTGLI
                     SDIYDILGITIKFGVAIIGCGLFLNLINCIRSKRYFSFLFDKGGLLGSWFYAGGVYAA
                     FFFVNSNYKSFPPDKELLILTG"
BASE COUNT         2201 a         1405 c         1334 g         1955 t
ORIGIN      
        1 taaaccctct gccgacctgg caacttgccc aagccaactt attgtagagg taagcgcgga
       61 taaaagaatt tggagtgctg ttattgatca gcgccatttt tttccagaca gtgataaact
      121 ctttttatgg aattttaatt cgatacctgc cagataccta cgtttcgaga tatcacctat
      181 cgaacttaaa aaaggtaatt ttttcatctc tattcatgat atagagatac atgccctacc
      241 cgaaggactt aatcactcac acacattcag cgagggtcca tcatacgcct ctgtttttca
      301 gcccggcctt gttcgttttg ccaaggacgg agaggatagc cgtgatgctg caattcgagc
      361 tagcgaccgc cgtctgcgcg atgcaacaac ctttttcaaa gggatagtcc agttcgcaga
      421 agatggacaa gcaactccaa atacagccat actagcatca gattcacgac tgaaacctgc
      481 tacggagctt tcagcgggca ttgttcaatt ggcatatgat tatgagataa aacctggaac
      541 agttgtacag gcatcagatc ctcgtcttaa agacgcgagt gaaaaaagtt ctggcattgt
      601 aaaactttgt cctgatggag catactctga gcttggagtg gttcgtggca acgactctcg
      661 ccttaaaaat gctactgaat cagcttttgg tattgttagg cttgccaaaa acggagaatc
      721 tgaaggcagc tctgttgttc aggctaatga cagtcgtttg cgtgacgcat ctgagatttc
      781 aaagggcatc gttgcatttt ctgctgacgg tgaagaaatt gaaggaaaag ctgttcaagc
      841 aaatgatcga cgcctgaaaa atgctactac aaaatcaaaa ggaattgttg aactagccga
      901 agacggagaa gactctccat taaccgctgt acaaggtaat gataaacgat taaagaatgc
      961 ttcaactgaa agccgtggaa ttgtggaact ggctgaaaac aacgagataa aggctggggc
     1021 tgtcgtacaa tcaaacgatg atcgcttacg tgacgcatca acaagttata aaggtatagt
     1081 tcggcttgca gaagacggcg aagaatcacc ccttgcggcc gttcaaggta atgacaagcg
     1141 gctaaaaagc gcgactaccg ttgcagaagg catcgtagaa ctcgcggaag atggagaagt
     1201 tgctgaatca aaagttgtac agagcaacga caaaagatta cgtgacgcta caacgacata
     1261 taagggcatt gttgaaatgg ccgaggatgg tgaagttgca gccgagagag ttgtacagag
     1321 tgatgatcgt cgtttaaaag acgcaactac caaaacaaca ggtatagttc aattttcaga
     1381 aaatggcgaa tcctctccat taaaagctgt acaaagcaat gatgaccgct tacgtaaatc
     1441 gtcagaagat tatccgggaa ttgtgcaact tgcaaaaaac ggcgaaaact caccctttaa
     1501 atgtgttcaa tcaaacgacc accgtctaag agacggttcg gaagaacaaa aaggtattgt
     1561 ccagtttgct cacaacggtg agatatcgcc cttgaaagct gttcagagtg acgatcagag
     1621 gatacaaccc gccggaacag taaccgcggg catagtaagg cttgccaaag acggagaatc
     1681 aacagagggt actgttgtgc aatcaaatga ttcccgactt catgacggca gcgaagatga
     1741 gaaaggtatc gtacgcttcg cgaagcatgg cgaatcacgt gcccaatttg ccgttcaaag
     1801 ttctgaccct agattaaacg atgctcgtga accccttaat catgaacata actacgcaca
     1861 aaagaatcac aactataacg aacatagcgg tactctctca atttcaggag agaaggcaga
     1921 agtattcgga ggcttaaccc caccatctga caattcagtg ataatttacg gaaaaaactc
     1981 ctcttcggaa gcaggagcca taggcgtatc cggagtggct ctaccttcta aaagtaaaaa
     2041 aaatgaattt tcaggttacg gtgttgtggg acattctgat tttgtaggca ttagaggcca
     2101 agctaccgga catgaaaaaa accctcgtgg agcaggcgtt cttggaatat caagaaacgc
     2161 accaggagga gtctttatta gtgaacacga ctattctctt cttgtagacg gatacgcatc
     2221 cattgacgat attgatccat ctcttaaaat ggttggaaac ggcaaagccc ttcaggttaa
     2281 aggagaatcc catttccatg gtgtaattca tattgacaat gacaaagata acgatgccac
     2341 aacaaatatt gtagagatgt ttgaacttga aaacgatgat tttatctcac atggagatgt
     2401 tgtgatagct tcagaaaagg gggcaagcct tttaaaacgc acacataagg cttatgatcg
     2461 cacagttatc ggaataattg ccgggaaacc cgcactctct ataaacaact ctggcaaaga
     2521 gacaaaactt taccctatcg ctcttgccgg taaggttatg tgcaaggttg atgcgagaac
     2581 acagcccatc gcaccgggag atcttttagt tacatctgac acacccggat gcgcaatgaa
     2641 aggaacaatc gattctttcg agaaagttgg ttcggttata ggtaaagcgc tggacagctt
     2701 agaaagcggt actggtgtaa tagcagtatt tcttactcac caataatact gaacaaccac
     2761 cgccctaaaa ttcatagggc ggttaaatac ttttttttat ggttttattc tattaagagt
     2821 gtcaatttct ttacggttaa atattttata attataatct ttaagttttt ttgaaacttc
     2881 cttgctgaca tagacaatct ttcggcaata aggttttttt tcgtaagcgt caatccatgc
     2941 agaaatctgt gttgtttcca gatcccatat tatcactcca ctgttgcgca ctatcgactc
     3001 ggcagtaggc tccccatatt tttcgattaa tttcttttta acatcttcca tcgccaaata
     3061 ggggaaagaa gttatgccat ataaatactg agcttcacct gttttttcgt catctgtcgc
     3121 accttctgtg gtagctcctt ccgtggtagc accttctgtt gccgcaccgg ctgttgccgg
     3181 ttccggttcc atttcttctt cgggatccat accaagcatt tcgggatctt cataaaaaaa
     3241 tccataagtg taagttattt caccgtcttt ggtgattata taatgatcct tacggtaaaa
     3301 ctgaagctta cctcgcgcga cctctcgaac ttcatcgaaa ctgcttcccc aacgtgctcc
     3361 accgggttca gtagcaacta ctgttgcctt atccggttct tcttcagact gacctaaaag
     3421 aatgccggta atagataaga caaccgttaa aataatgatt tttttcataa cttcccccgt
     3481 taaaaaaaac tgtcttttct caatatcggt ctatttagaa atttttaaag catattaagt
     3541 tacttgtgaa caaattatta ttggtggtaa cctctattaa tttaattaaa tatgcaatca
     3601 gtcaaacatc tctttgttag taaagttgtg ttggacaaaa aaacaagcga acttacataa
     3661 atttattgta cacacccaaa tatcgctaaa aagcaaaaag ttttttaact acccgaaaaa
     3721 taacattttt acaggttcca tgagaagatt gaaaagatat tgacattctt aagtcgtata
     3781 cctacaataa ggtgaaaatt tgtccgttat ttcttgtgca aacatctttc gggcataaaa
     3841 ttactatttg cggagcattt ctaatgaaag ctgtactgca agagattctc tcagccgaaa
     3901 aagaagcaga aaaaattgtt cgtgatgccc gcgacaaagc ctccaaaatg atcagagaaa
     3961 ctgatgccaa aataaacgaa gaaaaaatca aactcaaaga taatattcgc aaatctttgc
     4021 aaaaaagaat cattgcagca gcagagaatg tatatcaaga aaatgaaaac aaaagtattg
     4081 gtaaccatga tttacctaat aacagctatg aactcgcaat taatgaaata tcggctataa
     4141 tttgcaacac cagcgtaaag gaagaataat attatgggtg gcttatcaga ttatgcctac
     4201 ataaactcac ggatacatgc cctgatggca aaactcctcg ctcccgattt ttttgaaaaa
     4261 cttcttaaat ctgcttctct cgaagaagct ctctatatgt ttcaatcgac tgattttaat
     4321 tttattccaa agacttactc ccgcaccgga gatttgaaaa tagttgaaaa agaacttatt
     4381 acttttttat atcaagatgt tattaagctt caaaagcatt cgccagcaat acttattcca
     4441 cttgttaaag cgctttccga gcgcagccgt tacagcgcct tcaaaacagc tcttaggctc
     4501 tggtttgatc aatcatttcg caaaagatca atcagcgatt atactgagta tattcctcgg
     4561 gatatccctt tagaaggtgt ttcttacgac agtataataa acgcatcgaa caccgagtcc
     4621 cttaccgaac atctcagaac aaccccatac gcttgtgtca ttcaggaact ggaaaaaatt
     4681 agcgaaagtg gcaatctgct tcgctgtgaa attattcttg aaaacagcta ttacaaatat
     4741 ctttataaca cagtaataag ctactctctt tccacctgtt caacgctttt tgctaaaatt
     4801 catttttaca acaactttgc aaacatgcaa tactcatctt cttgcttgtg caatagtctg
     4861 aatctttttc acagcgacaa gaataccgac cttaaactcc tacttgaaca ggatatcgat
     4921 atgattaatc taggacggct tgttcgttta tcaaatattg ttagcccaga atcacagaat
     4981 attctttata ttcctggagg caaccgaatc atagagtcag attttattaa tgataatagg
     5041 ccatccacag cagaaatttt aaatcgtttt tcgctttcat ctatgactga caaattaacc
     5101 aaattggaac agcacgaact acatgcatat ataaaacaaa gcatacagca aaaaacaata
     5161 aacttttata caagagtaaa aaccagatca ttatttgacg agtctttagc atttgtgtat
     5221 atacgccttc gagaaaatga gatctccagg attatttcta ctttgaatac tctacactat
     5281 actgctaata ttcacggagg caactgatga tctttccgca atcaatgatc agattaaacg
     5341 caataatatt acgtaaagat accgattccg ttactaaagc actattaaat gaaggtatta
     5401 ttgattttac cgatatacat gataaaacga tctcattttc caatcaactc tcgccatttg
     5461 acaaagattc agctgaaaca gatatcattg aaatacgttc cagaatagaa caattattgt
     5521 cacaagtaaa tattcctctt cctgacacct cggatcttaa tgccgaaaag ctggttccgg
     5581 ttgatcccaa agaagttacc gaccgccttg atgaactgaa tcttcaaatt cggcaaattc
     5641 gcgacaagca gaaggatatt caacagagca tcaatagaaa aacggagata atgagacaaa
     5701 ccgatctttt tgtttccatc gaagcgtcac ctcctcctga gtcttccgtc agagtggcaa
     5761 caggtctgat acgtaaatcc aggaaggaag gattaactca ggctctatcg ttatatcctg
     5821 ctgttattca cttttcccaa tcaactcaag atgaaatatc agcaataatc atatatatgt
     5881 ccagagacga agaacgaatc ggcacggctc ttgacaggta tcagtggcaa agcgttgaat
     5941 ttccacctga attaacagga aagaaagaag agattcttca taaactcgcc tcttccatcg
     6001 aagaactaaa ccacgagcaa atcggatttg aaaaagaggc agaagatata attagaaaat
     6061 ttgctccgat acttaccgaa tcttggaaaa atctgacaat aaccgctcaa tgtataaaaa
     6121 ttcaaaatag ctttggtaaa acatcagaca ctgtaatttt ttccggctgg ctaccttgcc
     6181 atttgaaaaa tgcagctgaa aagatcatta agaaaattac gaatggaaag tattacatac
     6241 aatgggttaa acccgaagat gatcctttac tcaacagctc gattgttcct gttaaactaa
     6301 atcatggtac ttttcttaag ccgtttcaat gggtggtcga aaactattcg atgccgcaat
     6361 atggaagcat tgatccaact gttattgttg cattctcttt tttaactatg tttggattga
     6421 tgtttggcga cgcgggtcac ggtcttgttc ttgctctttg tggagcaatc gccgcgttta
     6481 taaaacgaaa aaaacacgat actgcctttg aacttgcgag gcttattatg tactgtggtt
     6541 tatcctcggt agttgctgga gctcttttcg gttcatattt tggacttggg atttttaaac
     6601 cactatggtt tgactatcat ggaattgtac tgggtcataa gggaactggt ctaataagcg
     6661 atatttacga tatactgggt ataacaataa aatttggtgt cgcgattatc ggttgcggac
     6721 tttttttaaa cttgataaac tgtatacgtt caaaacgata cttttctttt ctctttgata
     6781 aaggcggtct tttaggcagc tggttctatg ctggtggcgt gtacgccgct tttttctttg
     6841 taaatagtaa ttacaagagc tttccgccag acaaagagtt attgatttta acagg
//
LOCUS       DAOTGZ010000148         6870 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_3064
            NODE_13128_length_6870_cov_6.264563, whole genome shotgun sequence.
ACCESSION   DAOTGZ010000148 DAOTGZ010000000
VERSION     DAOTGZ010000148.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868088
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 6870)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6870)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:22:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,785
            CDSs (total)                      :: 2,751
            Genes (coding)                    :: 2,732
            CDSs (with protein)               :: 2,732
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 12 of 19
            Pseudo Genes (internal stop)      :: 5 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6870
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13128_length_6870_cov_6.264563"
                     /isolate="AMR_MDS_3064"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..560
                     /locus_tag="P5123_09670"
     CDS             <1..560
                     /locus_tag="P5123_09670"
                     /inference="COORDINATES: protein motif:HMM:NF013197.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRX16572.1"
                     /translation="SLAAPAGGFITSALIAAKKDDKQISTCCCETRPDFSGSRIMAFD
                     LKNEGLETQVIPDIQSHYVMKKKLISRVVVVADSIFEKGVFRAHCGCAMLVLAARSLG
                     VPVDLVIPGFLFDKRDYAEVEIEANRASINEVAIVNNGVVAPEGIVIGNYLSDTVDCS
                     DISRFITDKGVFDNWQNTLASLKSS"
     gene            complement(566..1336)
                     /locus_tag="P5123_09675"
     CDS             complement(566..1336)
                     /locus_tag="P5123_09675"
                     /EC_number="3.4.23.-"
                     /inference="COORDINATES: protein motif:HMM:NF013632.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004190 - aspartic-type endopeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="A24 family peptidase"
                     /protein_id="HRX16573.1"
                     /translation="MNSMHVSVASLTGLFFASFLLALADRFISKKYVNNIKGFLCDRS
                     SCDHCGTTIPFYYNIPLAGYIISLGHCRSCNNRISPRYPLSEAYGLILALFSLYRFGF
                     NIQALIFFSTVAVCSAVVYADIKTMEIPDSLLCVLIVAGILFALNGGFTKDSWLGLAS
                     AGGAFALFLVVFPGSFGTGDLKFAALLGFFTGFQFVIVMLETALITGSIFGAVYAITS
                     GRGLRIKIPFAPFIAIGYIIAILYGADILILYHNAFAP"
     gene            complement(1329..2771)
                     /locus_tag="P5123_09680"
     CDS             complement(1329..2771)
                     /locus_tag="P5123_09680"
                     /inference="COORDINATES: protein motif:HMM:NF020650.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HRX16574.1"
                     /translation="MSKISLKTLLLILIFSYSCSQPFSSQKPDNTVTKSLITESLTEK
                     RELSIIVNSYEQNIPLIYANGGFQTATNSFMAKKEISASISQKQSAEESLALLIAGKA
                     DIAVMPLFEYLSARKLKNDDSTIIFMLTGYSSGCHSIVAHSSFSRPTNLKGQKVALVQ
                     NSAEQILLDFFLKNNNVTPDNIQTVYTNTSLEALELFKSKKVQAAALSSFADIPESTL
                     ISTRDASKLIPYVAVANNSFLLSYPDLIRNFTSAILTAGSDFSGNSNDFSSIIKSGLL
                     PEQFARNETIKITQPASYSDNISFFSIDETTVTDINTLLNLSQLLPAQFYEQTTKSIF
                     SIYTPLVIEAVDIPNKIKNISLNRLYAPRSSAYPLIPILLPFKDDNWDITSFHERKLT
                     KITEIATLFPSSVIRLSYIPNRNANSRYAAEQRISVCSKRLYQIMRNRANLRIETNYA
                     SIAAVNSSSYSGPYEELSTVLVEIMAVSNE"
     gene            complement(2848..3153)
                     /locus_tag="P5123_09685"
     CDS             complement(2848..3153)
                     /locus_tag="P5123_09685"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRX16575.1"
                     /translation="MDQIRIWKKIDINDIEGHLLICDDLNAYCSSCKKMGVPIDAKTC
                     PGCNTTLDFITTHEKHNTTTGAQILLKMLQKSQKTVIEYSDYKHASDKKKAKNLFNS"
     gene            3558..4781
                     /locus_tag="P5123_09690"
     CDS             3558..4781
                     /locus_tag="P5123_09690"
                     /inference="COORDINATES: protein motif:HMM:NF014081.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HRX16576.1"
                     /translation="MKKWAIESDVLITPGKPVTKGKIVINNGVIEDVTSRDANSRMLL
                     TIKDGIISPGLINSHDHLMGSYYPKVGNGPYENWLPWDNDLKSAPVYEERQQIANRDL
                     YLIGSYRNLLSGVTSVQDHIPHFVQEPYLDILPIKLISDFAIAHSIASFALNWGGDIQ
                     EEYAYAAANSLPFITHIAEGFDDETRSDLQTLKSKKGFGPNSVLVHGIAFSEQDISEI
                     ASVGASVVWCADSNMFMFNKTANVREMLAAGVNVCIGTDSPMSGGLNILHEMRYCKKV
                     YNELYQEELPCRQIVKMATENPGKAFFLDSGRIEKGMLADITVFRNQGGDPYESIVNA
                     SLADVRLVVIEGMPVYGDAEFAEVFAQQDIEVQHVVVDGSEKVVIGNLTGLLDRVNRA
                     VGFNKKFPFLPVAEA"
     gene            4921..6129
                     /locus_tag="P5123_09695"
     CDS             4921..6129
                     /locus_tag="P5123_09695"
                     /inference="COORDINATES: protein motif:HMM:NF012257.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic nucleotide-binding domain-containing
                     protein"
                     /protein_id="HRX16577.1"
                     /translation="MSDVISGGGIRTRTYKSQAIVYFEGDKSESIYILKKGKVLLTYL
                     RPETGEEIKEEVRPGEFFGVKSALGKYPREENAQTIGETVILILSPVDFERIVLSNIQ
                     VVKKMLRVFSNQLRRIGRAVREVMGETNSVDPANELYKIADHYSGSGRPQQALYAYKK
                     YMEYYPDGKYASNAMRMIRNIESGNYQAPVVAVADEPVMSRPSVSESDDDFFASESSA
                     GAGSSFDDGNANSEFSDFDDYDDLGGLSDFGTEMDSFVSNSPDDGMMDDFGLGEPLSS
                     SPEDKYNEAVAFRNNGDCTHAIKLLDEIKADKSVSDPDLLHKVFIETGRCKLEQGKLQ
                     EALSELSLFLKNYPRSPFVKEALLEAGNVYLKAGSKDKAMAYLKKVAAMPPRDELNQQ
                     ALALLKQHKF"
     gene            6154..6798
                     /locus_tag="P5123_09700"
     CDS             6154..6798
                     /locus_tag="P5123_09700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015681348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0030552 - cAMP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HRX16578.1"
                     /translation="MLLDDHFLERFGVEYAPGEIIFCEYEPGHEFYLIHSGSVKIVKL
                     INNREKIMDVMYAGDVFGEQAILEAEPRSATVIAAEPTKLLKFHRDNFDALLQGNPQL
                     AFKLLVIFSKRIYDAKRRLMILLLNEPALKVMDVLCMLAEANTSVDLHSQVVLETSVK
                     DISSWSGMSVEETQNALTHMVNLGKIEMYPEKIVVKNILDFQRQVNSRRKQMFS"
BASE COUNT         2018 a         1172 c         1582 g         2098 t
ORIGIN      
        1 catcacttgc tgcaccagct ggtggattta ttacctcagc tcttattgca gcaaaaaaag
       61 atgataaaca aatcagtaca tgctgctgtg agacaaggcc tgatttttcg ggttcccgga
      121 ttatggcttt cgacttgaag aatgagggtc tggaaacaca ggttattccg gatatacagt
      181 ctcactatgt aatgaaaaag aaactgatat ctcgtgttgt tgttgttgcc gattcgatat
      241 ttgaaaaagg agtttttcgg gctcattgtg gatgcgcaat gcttgttctg gctgcaagat
      301 ctttgggggt tccggttgat ctggttattc ccggattttt atttgataaa cgtgattatg
      361 ctgaagtaga gatagaagct aaccgggcct caataaacga agtggcaata gttaataatg
      421 gggtagtggc tcctgaagga atagtcatag gtaattacct aagtgataca gtcgactgtt
      481 cggatatttc aagatttata acagataagg gtgtttttga caattggcaa aatactttgg
      541 caagtctgaa gtcctcttag ggtttttaag gagcaaaggc attgtgataa agtatcagaa
      601 tgtcggctcc gtaaagaata gcgatgatgt accctattgc tatgaatgga gcaaaaggaa
      661 tttttattcg aaggccgcgt ccgcttgtta tggcgtatac agctccgaat attgatcctg
      721 taatcaaagc tgtttcgagc attacaatta caaactgaaa acctgtaaaa aatccaagca
      781 aagccgcgaa tttaagatca cctgtaccaa aggaaccggg aaacactact aaaaaaagag
      841 cgaaagcgcc tcccgcgcta gctaacccca accaagaatc ttttgtaaaa cccccgttta
      901 gcgcaaagag tatacctgct acaataagca cacaaagcag tgaatctggt atttccatgg
      961 ttttaatatc tgcgtatact acagcgctac agacagcaac ggttgagaaa aaaataagag
     1021 cttgaatatt aaaaccaaac cggtaaagag aaaaaagtgc aagtataagc ccgtatgcct
     1081 cggaaagagg ataccttggt gatattctgt tattacagga acggcaatgt cccagtgaaa
     1141 ttatataccc tgccaacgga atgttataat aaaagggaat tgttgtgcca caatgatcgc
     1201 acgatgaacg gtcacaaaga aaacctttaa tgttgtttac atattttttg ctgataaaac
     1261 gatccgcaag tgccaacaga aagcttgcaa agaataggcc ggttaaagag gctacagaaa
     1321 catgcatact attcattaga cacagccatt atctcgacaa gtacggttga caattcttcg
     1381 taagggcctg aatacgatga agagttaaca gccgcaattg aagcgtagtt ggtttcgatt
     1441 cgtagattgg ctcggttgcg cattatctgg taaagacgct tgctgcatac agatatccgt
     1501 tgttctgctg catagcgtga attggcattt ctattgggaa tgtacgacaa ccttattact
     1561 gaggagggga acagggttgc tatttcagtt atttttgtca gttttctttc atgaaaagat
     1621 gtaatgtccc agttgtcatc tttaaacggt aaaagaattg gtattagcgg ataagcagat
     1681 gagcgaggcg cgtataatct gtttagtgaa atgtttttga tcttattggg tatgtctaca
     1741 gcttcgatta ctagtggtgt atatattgaa aaaattgatt ttgtagtttg ctcgtaaaac
     1801 tgagcaggca aaagttgaga gaggttaagc agagtgttga tatcggtaac tgttgtttcg
     1861 tcgattgaaa aaaaacttat attgtctgaa taggaggctg gctgtgttat ttttatggtt
     1921 tcatttcgcg cgaattgttc gggtaataat cctgatttga ttattgacga gaaatcgttt
     1981 gaattgcctg aaaaatcgga accagcggtt aaaattgccg aggtgaaatt tctaatcaga
     2041 tctggatatg ataacaaaaa actgttattt gcaactgcaa cataaggaat aagctttgat
     2101 gcgtctctgg ttgaaattag agtggattct ggaatatcag caaacgaaga caatgccgct
     2161 gcttgtactt tttttgattt gaaaagttcc agtgcttcca gggatgtatt ggtataaaca
     2221 gtctgaatat tgtctggtgt aacattgttg ttttttagga aaaaatccaa cagtatttgt
     2281 tcggccgaat tttgaactag agcgactttt tgccctttta gatttgtcgg ccgtgaaaaa
     2341 gacgagtgag ccacaattga gtgacagccg gaggagtaac ctgtaagcat aaaaataata
     2401 gttgaatcgt catttttaag ttttcgcgcg ctaagatact cgaacagtgg cataacggca
     2461 atatcagctt ttcctgcaat aagaagggcc aaactttctt cagcggattg tttctgactg
     2521 atggaggccg atatctcttt ttttgccata aatgagttgg tagcagtttg aaaaccgcca
     2581 ttggcataaa ttagtggtat gttctgttcg tatgagttaa caattataga taactctctc
     2641 ttttctgtta atgattccgt gataagcgac tttgtcacgg tattgtcagg tttctggctg
     2701 ctaaagggtt gcgagcagct atacgagaaa atgagtatta ataataaagt ttttaatgaa
     2761 atttttgaca tataaactct ctgttctaga taacaaggtt ttaacagtaa taagcgaaat
     2821 gaagactgaa caagtaatga gtttctgtta agaattgaaa agattcttgg ccttcttttt
     2881 atctgaagcg tgtttgtaat cggaatattc aataactgtt ttttgtgatt tttgtagcat
     2941 tttcaaaaga atctgtgcac ctgttgttgt gttatgtttt tcgtgcgtag ttataaagtc
     3001 cagtgttgta ttgcaccctg ggcaggtctt ggcatcaatg ggcactccca tttttttgca
     3061 agagctacaa taggcgttaa ggtcatcaca tatcagcaga tgcccttcta tatcgttaat
     3121 atcaattttt ttccagattc ttatttgatc cataacgaga gctccagtta atatataata
     3181 aaaatatcac tttaaagata gtgatagttt ttcacttttt taaatttttc tcgttgccac
     3241 tcaaaaaaac gctcaaaact atcactttaa agatagtgat agtttttcac ttttttaaat
     3301 ttttttcgtt gtcactcaaa aaaacgctca aaactatcgc tttaaaaata gcgatagttt
     3361 ttcacttttt atctattgtt gttgggttgt caataaggaa agaaagtgtt tggaaaattg
     3421 tctttatagc ttaaaactgc tgtaactaat tttacttgac tgtataggca cacggtaata
     3481 gtttttgggg aggttctaca gcaaaaggaa ccaactattt tctatcgttt atttgggtag
     3541 acctgtcggg gaaaaatatg aaaaaatggg ctattgaatc tgatgttctt ataactcccg
     3601 gaaaaccggt tacaaagggc aaaatagtaa taaataatgg tgtaattgaa gatgtgactt
     3661 caagagacgc taatagccgt atgcttctta caataaaaga cggaataatt agtcctggcc
     3721 tgataaattc gcatgatcat ctaatggggt cgtattatcc aaaggtcggc aatggtcctt
     3781 atgagaactg gttgccatgg gataacgacc tgaaaagtgc cccagtttat gaagagcgtc
     3841 agcagatagc gaacagggat ttgtatctga tagggtcata ccgcaatctg ttatctgggg
     3901 tgacgtctgt acaggatcat atcccccatt ttgtccaaga gccctatctt gatattttgc
     3961 caattaagct gatttctgat ttcgcaattg cccattcgat tgcttctttt gcccttaatt
     4021 gggggggaga tattcaagag gagtatgctt acgcggctgc caactctttg ccgtttatta
     4081 ctcatatcgc cgaaggtttt gatgacgaaa cgcgttcgga tcttcagacg ctaaaaagta
     4141 aaaaaggctt cggacctaac tctgttcttg ttcacggaat tgcattttca gaacaggata
     4201 tatccgagat cgcgtctgtg ggggcttcgg ttgtatggtg tgctgattcc aatatgttta
     4261 tgtttaacaa gactgcgaat gtgcgagaga tgcttgctgc gggggttaat gtttgtattg
     4321 gtactgattc tccaatgtcg ggtggactca atattttaca tgaaatgagg tattgcaaaa
     4381 aagtttataa cgaactttat caagaggagc tgccttgccg tcaaatagta aagatggcga
     4441 ctgaaaatcc gggtaaagcg ttttttctag atagtggtcg cattgaaaag ggcatgctcg
     4501 ccgacattac agtttttcgc aaccagggtg gagacccgta tgagtcaata gtaaacgcgt
     4561 cgcttgcaga tgtgagattg gttgttatag aaggtatgcc tgtgtatgga gatgccgaat
     4621 ttgccgaggt tttcgcgcag caggatattg aagttcaaca cgtagttgtt gacggttcag
     4681 aaaaagttgt gattggtaat ttaacaggtc tactagatcg tgtgaaccga gcagtcgggt
     4741 ttaacaagaa atttccgttt ttacctgttg ctgaagcgta gttgtctcag atattttgag
     4801 atgtaaagat ttgattgtta gttgacgata tttgattaag tgctcacttt ttttggtgaa
     4861 agtaatatat aacacttcac atttacattg ccgctttttt tacgcggtta cggagtcata
     4921 atgtctgatg taatatccgg tggtggaatt cgcacccgaa catataagtc tcaggcaatt
     4981 gtttatttcg aaggtgataa gagcgaaagt atctatattt taaagaaggg aaaggtgctg
     5041 ttgacttacc ttcggcctga aacaggagaa gagatcaagg aagaggtcag acccggtgag
     5101 ttttttggtg taaagtcggc tcttggtaaa tatcctcgcg aagagaatgc tcaaaccatc
     5161 ggcgaaactg taattctcat cctttctcca gtagatttcg aaagaatcgt gctttccaat
     5221 attcaggtag tcaaaaaaat gcttagagtt ttttctaacc agcttcgtcg aattggacgc
     5281 gctgtgcggg aagtcatggg tgaaacaaat tctgttgacc ccgcaaacga actatataaa
     5341 atcgcggatc actattctgg aagcggacgc ccacagcaag cattgtatgc gtataaaaaa
     5401 tatatggaat actatcctga cggtaaatat gcgtcaaacg ccatgcgaat gatacgtaat
     5461 atagaatcag gtaattatca ggcacccgtt gtggcggttg ctgacgagcc agttatgagc
     5521 aggccgtctg taagtgaatc tgatgatgat tttttcgctt cagagtcgtc agcaggcgct
     5581 ggttccagtt ttgatgatgg aaatgctaat tcggagttct ctgattttga cgactacgat
     5641 gatttgggtg gcttaagtga ttttggcacc gagatggact cgtttgtttc taacagtccc
     5701 gatgatggca tgatggatga ctttgggttg ggagagccgt taagttcgag tccagaggat
     5761 aagtataacg aagcggtcgc ttttagaaac aacggtgatt gtactcacgc tattaagtta
     5821 ttggacgaga ttaaggcaga caagtcggtt tcggatccgg atcttttaca taaagtattt
     5881 atcgaaacag gcagatgcaa gcttgaacag ggaaaacttc aggaagcact gagcgaacta
     5941 tcactttttt tgaaaaatta cccgcgttct ccatttgtta aggaggctct gctggaagct
     6001 ggcaatgttt atttgaaagc cggtagtaag gataaggcaa tggcctatct gaaaaaagtg
     6061 gccgcaatgc cgccgcgaga tgaactaaac cagcaggcgt tggcactttt aaagcagcat
     6121 aagttttagc cgtggctacc gcaggagagc aagatgttac ttgatgatca ttttttggaa
     6181 cgttttggag ttgaatacgc gcccggcgag ataatatttt gcgagtacga gcctgggcat
     6241 gagttttatc ttatacactc cggaagtgta aaaattgtta aacttatcaa taatcgtgaa
     6301 aaaataatgg acgtaatgta tgcaggtgat gtgtttggtg agcaggctat tttagaagca
     6361 gaaccccgct ctgcgacagt tattgctgcc gagcctacaa aactgctcaa gtttcatcgt
     6421 gataattttg atgcgttgct tcagggtaat ccgcagctgg ctttcaagtt gcttgttatt
     6481 ttctcaaagc gcatatatga cgctaaacgt cgtttaatga tactgctttt aaatgaaccc
     6541 gcattaaagg ttatggatgt cctctgcatg cttgcggagg caaatacttc agtggacttg
     6601 catagccaag ttgttctgga gacgagtgtt aaggatattt caagctggtc aggaatgtca
     6661 gtggaagaga ctcaaaatgc attgacacat atggtgaacc ttggaaagat tgaaatgtat
     6721 cctgaaaaaa tagttgttaa aaacatacta gattttcaac gacaggttaa ctcgcgccgc
     6781 aaacagatgt tttcataaac acaaaactta cgctcccgta gctcaggtgg atagagcagt
     6841 agtttcctaa actatgtgcc ggaggttcga
//
LOCUS       DAOTGZ010000149         6857 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_3064
            NODE_13158_length_6857_cov_3.656572, whole genome shotgun sequence.
ACCESSION   DAOTGZ010000149 DAOTGZ010000000
VERSION     DAOTGZ010000149.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868088
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 6857)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6857)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:22:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,785
            CDSs (total)                      :: 2,751
            Genes (coding)                    :: 2,732
            CDSs (with protein)               :: 2,732
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 12 of 19
            Pseudo Genes (internal stop)      :: 5 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6857
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13158_length_6857_cov_3.656572"
                     /isolate="AMR_MDS_3064"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..870)
                     /locus_tag="P5123_09705"
     CDS             complement(<1..870)
                     /locus_tag="P5123_09705"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRX16579.1"
                     /translation="MIFSKWTMFYLVLTLTVALSSCGSSDSDEDTKKTNPESGVTIAA
                     ASIANSSVLDTIPDSILTSIRNNFEVAYFHTSHGTHVSYGVFGLPDFKDGYDEKFAVS
                     TSDEPDKLYFRDNPGGFSVSDLSNADNDWEGWVEEARTWLEDPDNANVNVFIWSWCNI
                     ANHDATAYCNSMQTLIDEYGAGGSKVGAGLSRDNPVNFVFMTGHANVGNNTGDGQPKN
                     QAAIITDFCETNGYHCIDYYSIDTTAMDGTYYEDAGDNGNSAEYGGNFYEDWQASHTE
                     GVDWYSNRTSPGGS"
     gene            1172..1570
                     /locus_tag="P5123_09710"
     CDS             1172..1570
                     /locus_tag="P5123_09710"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRX16580.1"
                     /translation="MESYTETTWADFYDEAKKYNKLLKGALNKPEKFTPDFIYNIAAM
                     SVEKYFMAYFLKNSVLPPNHTIADLVMFMKQYQVIPDHLETRLLFMNDFQEICSLEQY
                     NRKTPTKDDTVKFADTVDMVFNYIDPMVAM"
     gene            1897..2475
                     /locus_tag="P5123_09715"
     CDS             1897..2475
                     /locus_tag="P5123_09715"
                     /inference="COORDINATES: protein motif:HMM:NF025879.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GyrI-like domain-containing protein"
                     /protein_id="HRX16581.1"
                     /translation="MGDNPSVYRKRKTFSQKEIFPRLSRASIESNKFLEQQTGELVER
                     EALLLVGIPFFYDISMKNDLSLPWSNLVKNINHIMERIIPERYYQVQYWFEDQDPGTL
                     FFFVAAEVKRIDDIPIQFTAKVLPRQTYLRFYHKGRANSVGNTYRYIYETFLPETEYR
                     LPYLYNFEQYGEKTLEPDNEESISEIYIPVSV"
     gene            2659..3282
                     /locus_tag="P5123_09720"
     CDS             2659..3282
                     /locus_tag="P5123_09720"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRX16582.1"
                     /translation="MKCKTIFCFITLFAVSFSTIKAESLNRISVGAGFGSFNTIIYGT
                     GFTATAIIDSELDKLDNKTEESTDEISAGVVFHASYDRKILQYLAIGFIYNYENINYI
                     INYDDNDFSETDLNIHTITPRITLIYGFKKLKIYHAFALGIGIYDNTLTEKIGSQIID
                     KEKMVYEPAIHVTIVGLEYHLTEDIYAFVDAGLGYAGFANVGVGYMF"
     gene            3428..3793
                     /locus_tag="P5123_09725"
     CDS             3428..3793
                     /locus_tag="P5123_09725"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRX16583.1"
                     /translation="MKCKTILCTLITVYVASFSSLSAENKNRFSAGIGSSSGYAFLET
                     LFFPSPGITIDSPNENIEGRIEDDTDFKSGLNLYNVSYDRMVFKHLALGFVYNYENIN
                     YIINYENMILLRLILRFTA"
     gene            3793..4056
                     /locus_tag="P5123_09730"
     CDS             3793..4056
                     /locus_tag="P5123_09730"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRX16584.1"
                     /translation="MPRIKIIHGFQRVKMYHALAVGFAIFDETLTEKVHFSTESYSKT
                     SYEPVWHCTLVGFEFHLTEDAYLYIENGYGYSGFFNIGAGLVF"
     gene            complement(4155..5321)
                     /locus_tag="P5123_09735"
     CDS             complement(4155..5321)
                     /locus_tag="P5123_09735"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF013884.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase"
                     /protein_id="HRX16585.1"
                     /translation="MFGENKKSENGTYYISNIRSTIVILVILLHAALSYSRVTDSWWF
                     IVDPSKTVIADILSGILDAILMPILFFISGYFSLPSLNKSTKKQFIKKKVKNIYFPWL
                     TGILFLNPVLVLIIKHTHGNDLYVSFCSILKYYNNFITIPIHIIQDRSHSSLYFSHYH
                     LWYLSLLFYFFVVFSLFQNTKQKINNIKSGSMLPTLLTLVMVSSVSFTFFYYFFENNW
                     FVLSIIQFQFSRIISYIAFFITGIHFYRSNYLADKKLLKYPFIHLLSAVALSIVYLSA
                     YEYVAKKNQIAINFIYAVCRYSLCVYFLVVCLSLGKKYFNNATTTNRILNRYSFGVYL
                     VHLNIVLVSAYLFRYFDTIPSLLKLCLTFLCSSAVSYLLVITYTRIKTLIISRL"
     gene            complement(5427..6443)
                     /locus_tag="P5123_09740"
     CDS             complement(5427..6443)
                     /locus_tag="P5123_09740"
                     /inference="COORDINATES: protein motif:HMM:NF015531.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S41 family peptidase"
                     /protein_id="HRX16586.1"
                     /translation="MKYLFSCFCISLLITSCALNWEDESDNPVNSFDYFWKEMKYYYP
                     GFIKSDLDWDSVYATYKPLIKDDPTNENLRKILIEITNMINDEHMRIYFSKSDIYPDL
                     PNSDEIFMHSIISNYVVFENSSHEEMYSYGFINDNIGYMWIKRFHGEGMSASAWAKDA
                     AKTIKSFEFCKALIVDVRNNDGGNLLNAEEILKYFITENIMNYSTQYWKTGPEYNDYQ
                     YNKSGSIKKADYTFNKPVILLMNTGCVSTTDFLGVLMKHNDLAILVGESNIISIIGDT
                     YPVELPNGWLVQFGRFAHEIYDGQYSEGDILQMDHEVSNAKEALNEGVDTQLEYALQL
                     VDSM"
     gene            complement(6440..>6857)
                     /locus_tag="P5123_09745"
     CDS             complement(6440..>6857)
                     /locus_tag="P5123_09745"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRX16587.1"
                     /translation="MEIYKLSSSLNGVNDLEYEFFQLGAAPAVKYEFQIMNHTISTGF
                     YFPVINYIVRRQWYIYEGMIENEYYDPLEVLVNCGEIAYANEYLKIPLKIKYKYSFSE
                     KFGISAEYEYTYISYENPRNTRSEYQSIIASIEVTL"
BASE COUNT         2262 a         1104 c         1236 g         2255 t
ORIGIN      
        1 gcttccaccg ggagaagttc tgttgctgta ccagtcaaca ccttcagtgt gggatgcttg
       61 ccagtcttcg taaaagtttc caccgtactc agccgagttg ccgttatcgc ccgcgtcttc
      121 ataataggtg ccgtccattg ctgttgtgtc aatcgaatag taatcaatgc agtgatagcc
      181 gttagtttcg caaaaatcgg taatgatggc tgcctgattt ttgggttggc catcgccagt
      241 attattgccc acgttagcgt gcccagtcat aaaaacgaag ttaacagggt tatctcttga
      301 aaggcctgca ccaacttttg agcctccggc tccatattca tcaataaggg tttgcatcga
      361 gttgcaatat gctgtcgcgt catgatttgc gatattgcac caagaccata taaaaacatt
      421 aacatttgcg ttgtcgggat cttccagcca tgttcgtgct tcctcaaccc agccttccca
      481 gtcgttatcg gcattagaga gatccgaaac actgaatcct cccggattat ctcggaagta
      541 gagtttgtca ggttcatcag aagtgctaac tgcgaatttt tcatcgtagc cgtccttgaa
      601 atcaggaaga ccaaacacgc cgtatgaaac gtgagtccca tggcttgtgt gaaaataggc
      661 aacttcaaag ttattacgga tagaagttaa aatggaatct ggtatggtat ctagtacgga
      721 actgtttgca atagatgctg cagcgattgt aacaccggat tctggattgg tttttttagt
      781 gtcttcatcg ctgtcggaag aaccacagga agagagagca acggttaatg ttaaaactaa
      841 gtaaaacata gtccattttg aaaaaatcat gtaaagcctc cttggagaat tattaaaaat
      901 ttccaagcgt ggagaacttc ggcagatact aggctggtag atcacaagta gaccagtttc
      961 ttatggtctt tcgatcttag taatataaca ataaatggat ctattgataa agtttgtcaa
     1021 ttatttatta cacagcagcg tttaattgtt ttggcagagc attctgtttc ctgcgtgtaa
     1081 aatagaaaaa tcggatggtt tattgtcttg ctttgtaatt gaagtggatg caactgataa
     1141 agcagcacaa taaaatggaa ggggttgaat tatggaaagt tacaccgaaa cgacatgggc
     1201 agatttttat gatgaagcaa aaaagtacaa taagcttctt aaaggcgcct taaacaaacc
     1261 tgaaaagttt acacccgatt ttatctacaa tatcgcagcc atgtcggtag agaaatactt
     1321 catggcatat tttttgaaaa atagtgtact tcctccaaac cacactatag cggatctggt
     1381 aatgtttatg aaacaatatc aggttattcc agatcatctt gaaacaaggc tgctttttat
     1441 gaacgacttt caggagatat gttcactgga acagtataat cgaaagacgc caacaaaaga
     1501 tgatacggta aaatttgcgg atactgtaga tatggttttc aattatattg atccgatggt
     1561 agctatgtaa aagatagtat ctaaaaagat attgacaccc gtatgataat tgctttattt
     1621 catgaatatg gaaacctcaa gttattattc tgataactat aaaatagatg ctttacccgg
     1681 ggaaagcact gatataggaa ttgtaagaat tgataaatct tattgatgtg acgtagtatt
     1741 ttaatatagt acaataaaga tcagaataaa aaatgtttac cgtaaaatat cggaagcggc
     1801 aaaagagata gttgccagtg aaaaaaaatt atcgagatag cctttgatta tggtttcagt
     1861 actcccgaat ctttttcacg tgcctttctg aaaattgtgg gtgataatcc ttccgtttac
     1921 cgaaaaagaa aaacattttc tcaaaaagaa atttttcctc gtttatcgcg tgcatccatt
     1981 gaatctaata agtttctaga gcagcaaacg ggtgagctgg tcgagagaga agctctgctg
     2041 cttgtaggca tccctttttt ttatgatata tccatgaaaa atgatctgtc tttaccctgg
     2101 agtaatcttg taaaaaatat taatcatatt atggaacgga ttattcctga aagatattat
     2161 caggtacagt actggtttga agaccaagat ccgggtaccc tttttttctt tgtcgcggcc
     2221 gaagttaaaa ggatagatga tattccaata cagttcaccg ccaaagtttt accgcgtcag
     2281 acatatcttc gtttttatca caaggggaga gcaaactctg taggcaatac ctacagatat
     2341 atttacgaaa catttttacc ggaaacagaa taccgcctgc cgtaccttta taattttgag
     2401 caatatggcg aaaaaactct ggaacctgat aatgaagagt ctatcagtga gatatatatc
     2461 ccggtcagtg tgtgattatt tttgctaata tcagggctaa ttgtaaactg ggtaaccggt
     2521 ggaaaagcgt attcaatatt atttcagtaa catctatgtc gtctgtgata aaataaatca
     2581 aaaaaaatct gtgttaattt taattgactt ttttcaatag tcaatttatg aactaaagta
     2641 atcaaagggg ataaactgat gaaatgtaaa actatttttt gctttataac actttttgct
     2701 gtctcttttt cgacaataaa agctgaatcg cttaacagga ttagtgttgg tgctggtttt
     2761 ggttctttta acacaataat ttatggaact ggatttacag ccacagccat tatcgattca
     2821 gaattagata aactcgataa caaaacagaa gagagtacgg atgagatatc cgctggagta
     2881 gtttttcatg cctcatacga tagaaaaatt ttgcaatatc ttgcgatcgg ttttatctac
     2941 aattatgaaa acattaatta tattattaat tatgatgata atgatttttc agaaacagat
     3001 ttaaatattc acactattac gccccgtatt acattgatat acggttttaa gaaactgaag
     3061 atataccatg catttgcgct tggaataggg atatatgata ataccttaac tgaaaaaatt
     3121 ggctcgcaaa taattgataa agaaaaaatg gtctatgaac ccgcaattca tgtcacaatc
     3181 gttggattag aatatcactt aactgaagat atttacgctt ttgtggatgc tggtttaggt
     3241 tacgctggtt tcgcaaatgt tggtgtcggc tatatgtttt aaataactgt atgtctcatt
     3301 attaatcaat atcttttatg aatatttatt atagtgaata ttcatgttgt ttgtttataa
     3361 attgttataa cagatatctg tttctatagt cagtttatga accagggtaa tcaaaggaga
     3421 aaaactgatg aaatgcaaaa ctattttatg tacactaatt actgtttatg ttgcttcttt
     3481 ctcttctctt agtgctgaaa ataaaaacag attcagtgca gggatcggaa gttcttccgg
     3541 ttatgcgttt ttagaaacat tattttttcc gagccctgga ataaccattg attcacctaa
     3601 cgaaaatatt gaaggtagaa ttgaagatga taccgatttt aagtctggtt taaacttgta
     3661 taatgtctct tatgatagaa tggtttttaa acatcttgct ttgggttttg tgtataatta
     3721 cgaaaatata aattacataa ttaattatga aaatatgata cttctgagac tgatcttacg
     3781 attcactgct taatgccgcg tattaaaata attcatggtt ttcaaagagt caaaatgtat
     3841 cacgcgttgg cggtaggttt tgcaatcttt gatgaaacat taaccgaaaa agtgcatttt
     3901 tcaacagaaa gttattcgaa gacatcctat gaacctgtat ggcattgtac tctagtaggt
     3961 tttgaatttc acttgactga agatgcttat ttgtatattg aaaatggata tggttatagt
     4021 ggatttttta atatcggtgc tgggcttgta ttttgaattt tacatcgtta tgtaaaacat
     4081 aattctaaat ttatcgataa taaatatact gcaattccgg gttttactat agcggcaaac
     4141 agaagattat aattttataa ccgggatata atcagtgttt taattctggt atacgttata
     4201 acaagcaaat aggatactgc ggaagagcat aaaaatgtca gacaaagttt taataaagat
     4261 ggtattgtat caaaatatct gaaaagatat gctgaaacca atacaatatt cagatgaacc
     4321 agatatacac cgaaagaata tctgttaagt attctgtttg tagttgtagc attattaaaa
     4381 tatttctttc ccaaagaaag gcataccact aaaaaataga cgcaaaggct gtatcggcaa
     4441 accgcatata tgaagttaat tgcaatttga tttttttttg caacatattc atatgccgat
     4501 aagtatacaa ttgaaagagc taccgcactg agtaaatgta taaaaggata ttttagtaat
     4561 ttcttatcag caagatagtt acttcggtaa aagtgaattc ctgtaatgaa aaatgcaata
     4621 taagaaatta ttctggaaaa ctgaaactgg attatagata aaacaaacca gttgttttcg
     4681 aaaaagtaat aaaaaaaagt aaatgaaact gaagaaacca ttacaagggt caataacgtg
     4741 ggcagcatcg atccggactt gatattattg attttttgtt ttgtattctg aaataatgag
     4801 aatacaacaa aaaaatagaa caataacgat aaataccata aatgataatg tgaaaaatat
     4861 agagatgagt gacttctgtc ctgaataata tggatcggaa tagtgataaa gttattgtag
     4921 tattttaata ttgaacagaa agaaacatat agatcgttgc catgtgtatg ctttataatc
     4981 aaaactagaa caggatttaa aaataaaata cctgttagcc atggaaagta tatattttta
     5041 actttctttt ttataaactg tttttttgta cttttattta aagacggcaa ggaaaaataa
     5101 ccagatataa aaaacagaat aggcattagt atcgcgtcta atatgccgga taaaatatcg
     5161 gcaataacag ttttagaggg atccacaata aaccaccatg aatccgttac tctggaatat
     5221 gacaatgccg catgaagcag aataacaaga atcacaatgg ttgaccggat attgctgata
     5281 taatatgtcc cgttttcact ctttttattt tcaccaaaca tatctttttc ctgttgatta
     5341 gttaattaac acgttacgtt taaaaaaaac tgtgttggaa taatacgcaa tcgggtaatg
     5401 aaaaccttaa gctgtatatt atatttttac atggaatcga caagttgcaa agcatattca
     5461 agttgagtat ctactccttc gttaagagct tccttggcat tgcttacttc atgatccatt
     5521 tgcagtatat cgccctcaga gtattggcca tcatatattt catgggcaaa tctgccgaat
     5581 tgcacaagcc atccgttagg aagctctact ggataggtgt cgccaataat agaaataata
     5641 ttgctttctc caacaagtat agcaagatca ttgtgtttca taagtacgcc tagaaaatca
     5701 gttgtgctaa cacaaccggt attcattagt aaaataactg gtttgttgaa agtgtaatcg
     5761 gcctttttta ttgagccgct tttgttatat tggtaatcat tatattccgg accggttttc
     5821 caatattgag tggagtaatt cataatattt tctgttataa agtatttcaa aatttcttca
     5881 gcatttaaca agttccctcc atcattgttt cttacatcaa cgattagagc tttgcagaat
     5941 tcaaaagatt taattgtttt tgccgcgtct tttgcccatg cgctagcgct catgccttcc
     6001 ccatgaaatc tcttgatcca catatatcca atattatcat ttataaaacc atatgaatac
     6061 atctcttcgt gtgatgagtt ctcaaatact acataattcg aaattattga atgcatgaat
     6121 atttcatcgc tatttggcaa gtctgggtag atgtcactct ttgaaaaata tattctcata
     6181 tgttcgtcat ttatcatgtt tgtaatttca attaatatct ttcgcaagtt ttcgttggtt
     6241 ggatcatcct tgatcaatgg cttatatgtt gcataaactg aatcccagtc aaggtcactt
     6301 tttataaaac ctggataata gtacttcatt tctttccaga agtaatcaaa agaatttact
     6361 gggttgtcgg attcatcttc ccagttcaag gcgcagcttg taattaataa agatatacag
     6421 aaacaagaaa ataaatattt cataaagtta cctcaatgct tgcaataatc gattggtatt
     6481 ctgatcgggt atttcgaggg ttctcatacg aaatatatgt gtattcatat tcagcgctta
     6541 ttccaaattt ttcgctgaac gaatatttat atttaatctt taaaggtatt tttagatatt
     6601 catttgcata ggcaatttct ccacaattaa ccaaaacctc aagggggtca taatattcgt
     6661 tttctatcat gccttcataa atataccatt gccgacgtac aatatagttt ataacaggaa
     6721 agtaaaatcc ggtcgaaata gtatgattca ttatttgaaa ctcatattta actgccgggg
     6781 cagcacctag ttgaaaaaat tcatattcaa gatcgttgac tccatttaag gaggagctta
     6841 gcttgtatat ttccata
//
LOCUS       DAOTGZ010000150         6790 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_3064
            NODE_13324_length_6790_cov_4.255234, whole genome shotgun sequence.
ACCESSION   DAOTGZ010000150 DAOTGZ010000000
VERSION     DAOTGZ010000150.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868088
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 6790)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6790)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:22:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,785
            CDSs (total)                      :: 2,751
            Genes (coding)                    :: 2,732
            CDSs (with protein)               :: 2,732
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 12 of 19
            Pseudo Genes (internal stop)      :: 5 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6790
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13324_length_6790_cov_4.255234"
                     /isolate="AMR_MDS_3064"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1562
                     /locus_tag="P5123_09750"
     CDS             <1..1562
                     /locus_tag="P5123_09750"
                     /inference="COORDINATES: protein motif:HMM:TIGR03904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="YgiQ family radical SAM protein"
                     /protein_id="HRX16588.1"
                     /translation="RVLEQQGYRVAICDMPDVKDASSFALFGAPRLFFAVTGGNVDSV
                     MMRYTSFLKVRNDDPFRPETEIERPLRSVISYCNCIRSLYKGIPIVIGGIEASMRRLA
                     HFDFIDNKIRRSILLDSRADILIYGMGEYAVKEVAKRVKKGIAIEGIPGTVIVRKELP
                     DDLKKYKVLPDEEILTAGDTESKKQYGIFFKTIYLSLQTVFVQGSGGRFLIQYPPYKM
                     TGDELDTVYELPYKYSPAPKYLNRKIDAFNMIQNSVNSHRGCVSGCAFCSIALHQGRR
                     VVSRSEKSIIREIETLTRDKNFKGHISDVGGPTANMYAVSCRSNWQCTRESCLYPNRC
                     PSLVVETKKYLKLLEHCRKIPGVKKITIGSGVRYDLFYSDYKEGLEDFIVNYVGGQLK
                     IAPEHTEPEVLHVMRKVPLVPLADFVMFFSKICKKRGVKYFLVPYLMSNHPGSSHASM
                     IRSRDNVKKLFGYVPKQVQSFFPLPLTLSSAVYYTGYDPLTGKKVFVEKDKGARNRQH
                     SLFFEHKKGKK"
     gene            1676..2422
                     /locus_tag="P5123_09755"
     CDS             1676..2422
                     /locus_tag="P5123_09755"
                     /inference="COORDINATES: protein motif:HMM:NF022441.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2807 domain-containing protein"
                     /protein_id="HRX16589.1"
                     /translation="MRKYIIGIIFIVLFMSSCENNRYKSGNIVTVQRSFNGYNGVSLE
                     GKGIIYLKQCDQFVFKAKAPEYVLDIIDVTVRKGVLTISYLENLSSDFIPEYTIELPQ
                     LSYLGVEGAAIVKMENTFTGNQITIESKGATAAEVYVDVNNLTIKAGGNSVVVLNGEA
                     SILEAEIKGKTELRARDFIAEKAVIKSTGFTKSEIGVKEFLDVTISGHGNVKYNGTPK
                     ISTSIAGAGRIESGNYEPARDQKQAATAEE"
     gene            2376..3224
                     /locus_tag="P5123_09760"
     CDS             2376..3224
                     /locus_tag="P5123_09760"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRX16590.1"
                     /translation="MNQQEIKNKLLQLKSDVADFTVILSGKKSGKVDGLYKPDSCEII
                     IHNKNFESENRLIYTAIHEFAHHVQFTESSGTISSKSHTTLFWNLFHSLLFDAEKKGI
                     YNSLFKTDSRFVELTKKIKNDYLGQNAELMKNFGNMLLEAMSLCMETQASFEDYLDRE
                     LGIKRQEAKALIKAFQHNVTSEIGIDNMKLVARIDDDDLRRRALAGFQKGMTEPMVKA
                     EVINPDKYKLQKQDELEVLMKERTRLEKSIAHLIERLENIDQRLSECGADLSNKLASD
                     QDEGDV"
     gene            3217..4608
                     /locus_tag="P5123_09765"
     CDS             3217..4608
                     /locus_tag="P5123_09765"
                     /inference="COORDINATES: protein motif:HMM:NF025030.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HEAT repeat domain-containing protein"
                     /protein_id="HRX16591.1"
                     /translation="MFNRIRAQWYYNRLEHSKGDKFIRSVSRLATIKSSNTITCLLDI
                     LISRNDLAINEKAALAEAFAAIGSEATRFLINSVYDENGMIRYNVVSALGHTKDKEAL
                     PLFMFLLEESDNTIFAKALGDIGSSESLPVLRSKVRQRSSHCRFESLRAIAAIDLSGN
                     SDIYCELLYDDEISIRVQAATYLRDISYKFTDTSDQMLFYAVFNEWNLVQIKDKKTAL
                     GLLHFVDRSDEILCEKIFERVTGYNYPDKTEVWLAYLQELPNELKRGFLDMIDYAPVS
                     MLDILVGLLSVADLHLKRSAEKLLCRSGEDAGRKALEALTNQDIKNELVRTSLIAVAV
                     ETLSDKITEISDYIKKANNRDLPVLLETAAECANPGLAPVIRDLLQKTDDEEIILCSV
                     YALGEIEDKESSGVVSSYLKHEDRDIRIAVYEALRKIGDRRVRSMLDDSLNEVADDEE
                     RELLKNALREISQ"
     gene            4605..5915
                     /locus_tag="P5123_09770"
     CDS             4605..5915
                     /locus_tag="P5123_09770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006928305.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008134 - transcription factor binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HRX16592.1"
                     /translation="MKLPDGIPAEILLDSIADGLFCIDADFRIIMINRAALDILDIEE
                     SDAIGRRCHEVFHASVCETSCVLRETIDSDKPIINREVYIVKEDGSRVPLSVSTSVIR
                     DNKGDVVAGVEIFRDMSMVENLRKAVLENYTFEDIISKNSQMHALFEIMPDIAESEST
                     VLITGESGTGKELFARALHSRSFRTDKKFVALHAAALPEQLLESELFGYVKGAFTGAN
                     RDKPGRIAEAEGGTLFLDEIGDIPPSIQVKLLRFLQFRDYEPVGSNITRRADVRIIAA
                     TNKDLSSGIANREFREDLYYRIAVIRIDIPPLRSRREDIPLLADSILARLQLMRGKGN
                     KTLSGEVKARLMAYDFPGNVRELENIIEYAYVMSRSDRIESSDLPSDIVSASDEEDLR
                     LDHVQNRHILRVLKANSGNRTETARQLGINASTLWRKLKDMDVV"
     gene            6326..>6790
                     /locus_tag="P5123_09775"
     CDS             6326..>6790
                     /locus_tag="P5123_09775"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRX16593.1"
                     /translation="MKIAIPVFNERLSPLLDTAYTFVVYSQKKRESAILTRTNDCSVS
                     RFLQMHGCNAVVYSRMSRDVADMLYGAGIFIISSGPFRIDKLLDLLSGSCLNADTFLK
                     SGCCRRRGVCVIKDGIIKENCMKIALTATGETSDCEVDQRFGRAKGFMIFDED"
BASE COUNT         2063 a         1118 c         1537 g         2072 t
ORIGIN      
        1 ggcgtgttct tgagcagcag ggataccggg tagctatttg cgatatgccc gatgtaaaag
       61 atgcatcttc ttttgctctc ttcggagctc ccaggttgtt ttttgcggta acaggtggca
      121 atgtagattc tgtcatgatg aggtacacct cgtttcttaa agttcgtaat gatgatcctt
      181 ttcgtccaga aacagagatt gaacgccccc tgcgatctgt aatatcttat tgtaattgta
      241 tacggtctct ttataaggga attccgatag ttatcggtgg tatcgaggct tcgatgcgac
      301 ggcttgccca tttcgatttt attgataata aaataagacg ttccattttg ttagattcgc
      361 gggctgacat actcatctac ggcatgggag aatatgctgt taaagaggtt gccaaaagag
      421 ttaaaaaggg tatagcgatc gagggcatac ctggaactgt aattgtacgt aaagagttac
      481 cagatgattt aaaaaaatac aaagttttac cagacgagga aattctaacg gccggtgata
      541 ctgaatctaa aaaacagtat ggtatttttt ttaaaaccat atatctttct ctacagactg
      601 tctttgtgca aggaagtggg ggtcgctttc ttattcagta ccctccttat aaaatgaccg
      661 gggatgaact ggatactgta tatgaattgc cctataaata tagccctgca ccaaaatact
      721 taaaccgcaa gatcgacgca tttaatatga ttcaaaattc agtaaattct catcgaggct
      781 gtgtttcagg ttgcgcgttt tgttcaattg ctcttcatca aggtcgccga gtcgtcagca
      841 gaagtgaaaa gagtattatt cgagagattg aaactctgac tcgtgataag aatttcaagg
      901 gtcatatttc ggatgttggt ggcccaacag ccaatatgta cgcagtaagc tgtcgctcta
      961 attggcagtg tacccgcgag agttgtcttt atcccaatcg ttgtccttct cttgttgtag
     1021 agacaaaaaa atatcttaaa ctgcttgagc attgcagaaa aattcccggt gtaaaaaaga
     1081 ttactatagg ttcgggagtg cgttatgatc ttttttatag tgattacaaa gaaggtctgg
     1141 aggactttat agtaaattat gtgggaggac aacttaaaat tgctccagaa catactgaac
     1201 cggaagtttt gcatgtaatg agaaaggttc ctcttgtccc cttggccgat tttgttatgt
     1261 tttttagcaa gatatgcaaa aaacgtggtg ttaaatattt tttagtgcca tatcttatgt
     1321 ctaatcatcc aggctcaagc catgcatcaa tgatacgatc ccgtgataat gttaaaaaac
     1381 tttttgggta tgtaccaaaa caggttcaat ccttttttcc ccttcctttg actctctctt
     1441 cagctgtcta ctatactggg tatgaccctt tgactggcaa aaaggtcttt gtcgaaaaag
     1501 ataaaggcgc ccgcaacaga caacattcac tattttttga acataaaaag ggtaaaaagt
     1561 agcgttttgt cgccactctc ttgttgtata aagcgataat aggtaataac tacaattcta
     1621 caggatactt ttctgcagag ctaaaagttc ttctatgttg gttcaggagg gggaaatgag
     1681 aaaatatatt attggcatta tatttattgt tctttttatg tcgtcttgcg aaaataaccg
     1741 atataaatcg ggaaatatag tgacagtgca gcgctctttt aacggttata acggtgtatc
     1801 tcttgaggga aagggcataa tatatttaaa acagtgcgat cagtttgttt ttaaggcaaa
     1861 agcccccgag tatgtgcttg atattattga tgtaacggta agaaaaggag tgttgactat
     1921 aagctatctg gaaaatttat cttcggattt tatacctgaa tatactatag agttgcctca
     1981 actaagctat cttggtgtag agggagccgc aatagtaaaa atggaaaata cctttactgg
     2041 caatcaaatc acaattgaaa gtaaaggcgc gactgcagcg gaggtatatg ttgatgtaaa
     2101 caatcttaca attaaagcag gtggaaactc agtagtagta ctaaacggtg aagcttctat
     2161 tctggaagct gaaatcaagg gaaaaaccga gttaagagct cgagatttta tcgcagaaaa
     2221 agccgtgatt aagagcacag gctttaccaa aagcgagatt ggggtaaaag aatttcttga
     2281 cgttacaata tccgggcatg gtaatgtaaa gtataacggt acaccaaaaa tatcaacttc
     2341 gatagccggt gccggaagga tagagtcggg aaactatgaa ccagcaagag atcaaaaaca
     2401 agctgctaca gctgaagagt gatgtagcag attttacagt aatattatca ggtaaaaaat
     2461 ctggcaaagt ggatggcctg tacaagcctg acagctgtga aattattatt cacaataaaa
     2521 atttcgaatc agagaacagg cttatatata ccgcgattca tgagtttgca caccatgttc
     2581 aatttacaga gtcatctgga acaatttctt ccaagtccca cacaaccctg ttttggaatc
     2641 ttttccattc cttgctcttt gatgccgaga aaaagggtat ttataattca ttgtttaaaa
     2701 cagacagcag atttgtggag ctcacaaaaa aaatcaagaa tgactatctt ggccaaaacg
     2761 ccgagttaat gaaaaatttt ggcaatatgc tattagaggc aatgtcttta tgtatggaga
     2821 cacaagccag tttcgaagat tatctggatc gagagctggg catcaaacgg caagaggcga
     2881 aggctctgat taaagctttt cagcataatg tgaccagtga gattggtatc gataatatga
     2941 agcttgttgc acgaattgat gacgatgatc tacgccgtcg agcattggcc gggttccaga
     3001 aagggatgac cgagccgatg gtaaaagcgg aggtaattaa ccccgataag tataaactgc
     3061 agaaacagga tgagctcgaa gtccttatga aagagagaac ccgccttgag aagagtatag
     3121 ctcatcttat agagcgtctt gaaaatattg atcagagact ttcagaatgt ggggccgatt
     3181 taagtaataa gctcgcttct gatcaggatg agggtgatgt ttaaccgtat tcgggctcag
     3241 tggtactata atcgtctgga acactccaag ggtgataaat ttattaggtc tgtatcacgt
     3301 ttggccacaa ttaaatcatc taatacaatt acttgcctgc tagacatact aatctcgcga
     3361 aatgatcttg ctataaatga aaaggcagct cttgccgaag cttttgctgc tattggctcg
     3421 gaagctactc gttttttaat taacagtgtt tatgatgaaa atggaatgat tcggtataat
     3481 gttgtaagcg cgcttggtca tactaaggat aaagaggctc ttcctctttt tatgtttctt
     3541 cttgaagaga gtgataacac tatttttgca aaggctcttg gtgatattgg gtcctccgag
     3601 tcattaccgg tattgcgtag taaagtaaga cagagatcat cacattgccg tttcgaatct
     3661 ttaagagcta ttgccgctat agatttgagt ggaaacagcg atatttactg tgagctgtta
     3721 tacgatgatg aaatttctat acgagtgcaa gctgccacat acttgcgcga tatttcttat
     3781 aagtttaccg acacttccga tcagatgctc ttctacgcgg tttttaatga atggaatctt
     3841 gtacagataa aggataaaaa aacggctttg ggattattgc attttgtaga ccgatctgac
     3901 gaaattttat gtgaaaaaat atttgagcgt gtaactgggt ataattatcc cgacaagaca
     3961 gaagtttggc tggcatattt gcaagaactg ccaaatgagc ttaagcgcgg ttttttggat
     4021 atgatagatt atgcgccagt gagtatgctt gatattcttg ttggccttct ttcggttgct
     4081 gatcttcatc ttaaacgatc agcagaaaaa ttgctgtgtc gctcaggtga ggatgctggc
     4141 agaaaggctt tagaagcttt aactaatcag gatattaaaa atgagttggt tcgaacctca
     4201 ttaatagctg ttgctgttga aactttgtct gataaaatca cagaaattag tgattatata
     4261 aaaaaggcga ataatcgtga tttgcctgtt ttgctcgaaa ctgctgctga atgtgccaac
     4321 ccaggattgg ctcctgtgat tcgtgatttg ttgcaaaaaa cagacgatga agaaataatt
     4381 ttatgcagtg tttatgcgtt aggcgagatt gaagataaag agtcatctgg tgttgttagc
     4441 agttatttaa agcatgaaga ccgtgatatt agaatagcag tatatgaggc acttagaaag
     4501 attggagata gaagagttcg atcgatgctt gatgactcac tgaatgaggt ggccgatgat
     4561 gaagaacgtg aattattaaa aaatgcatta cgggagattt ctcaatgaaa cttcccgatg
     4621 gtatacccgc tgaaatactt ctcgattcaa ttgcggatgg actcttttgt attgatgccg
     4681 attttagaat tattatgatt aatcgtgctg ctttggatat tcttgatatt gaagaaagtg
     4741 acgcgattgg aaggcgctgt catgaggttt tccacgcctc tgtttgtgaa acgtcttgtg
     4801 tgttaagaga gactatagat tcggataaac cgataataaa cagagaggtt tatattgtaa
     4861 aagaggacgg tagtcgagta ccgttatcgg tttcgacatc ggtaatcaga gataataagg
     4921 gagacgtagt cgcaggtgta gaaatttttc gtgatatgag tatggtggaa aatttacgca
     4981 aggcagtttt ggaaaactat acctttgaag atattataag caaaaatagc cagatgcacg
     5041 cactttttga gattatgcct gatattgctg agagtgaatc tactgtgttg attaccggtg
     5101 aaagcggtac tggtaaggaa ctctttgcaa gagcgctgca cagcaggtct tttagaactg
     5161 ataaaaaatt tgttgctttg catgctgctg ctttgccaga acagcttttg gagtcggagc
     5221 tctttggata tgtaaaagga gcttttactg gagcaaatag agataaaccg ggacgtatcg
     5281 ctgaagcgga aggtgggact ctttttttgg atgagattgg agatattcct ccttctattc
     5341 aggtgaagct gttgcgtttt ttgcagttca gggattacga gccagttggc agcaatatta
     5401 ccagaagagc cgatgtccgt attattgcgg ctactaataa ggatctatct tcgggaattg
     5461 caaacagaga gtttcgggaa gatctctatt atcgcatcgc ggttattaga attgatattc
     5521 ctccactaag atcgcgaaga gaggatatcc ctctcttggc tgactccata ctcgctcgtt
     5581 tgcagctaat gagaggtaag ggtaataaga ctctttcggg agaggtcaag gcgaggttaa
     5641 tggcatacga ttttccggga aatgtcaggg agctcgaaaa tattatagaa tatgcgtatg
     5701 tgatgagtcg atctgacaga attgagagtt ccgatttgcc gtctgatatt gtttctgcaa
     5761 gtgatgaaga ggaccttagg cttgatcatg ttcaaaaccg gcatattttg agagttttaa
     5821 aagcaaatag cggcaaccgt accgaaactg caaggcaact tggcattaac gcttctacgc
     5881 tgtggcgtaa actaaaagat atggatgtgg tttagatctg atttatattc catatcgtcc
     5941 ccaaagcttt gacttataac aatggattgt ggtacataaa gaaactattc tgaaattggt
     6001 ttctgataag ccaatttcag aataaggcat ttatatgttt gattaaaaaa ggaactaaca
     6061 tattccaata aaaagattga aatactgttt gtcttgtaac tttgtattga attatgtgga
     6121 ctttgtcttg caatatgcaa caaataatga caaacatatt gcatattgca agagttgata
     6181 aaaaaataac tctaatttca ttaaaataga ttcgtcatat acagtgatta gcgttaataa
     6241 tcctcttttt attcgccaaa cccataaatt tacctgcttt ctagcgtctt ttgataaatg
     6301 gcacgtataa tgcattaata actgtatgaa gatagcaata ccagttttta atgaacgtct
     6361 ttcaccccta ttggatacag cgtacacatt tgtggtgtat tctcaaaaaa aaagagagtc
     6421 tgcaatattg acacggacga acgattgctc tgtctctaga tttttacaga tgcatgggtg
     6481 taatgctgtg gtatacagca gaatgagccg tgatgtggca gatatgcttt atggagctgg
     6541 aattttcatt atttctagcg gaccatttag aattgataag cttttagacc tgctttcggg
     6601 ttcatgtttg aacgcggata catttcttaa aagtgggtgt tgtagacgtc gaggtgtttg
     6661 tgttattaaa gacggaatta taaaggagaa ttgtatgaaa attgcgctga ctgcaactgg
     6721 agaaacaagt gattgtgagg ttgatcagag atttggccgg gctaaaggtt ttatgatttt
     6781 tgatgaggat
//
LOCUS       DAOTGZ010000151         6777 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_3064
            NODE_13363_length_6777_cov_5.130467, whole genome shotgun sequence.
ACCESSION   DAOTGZ010000151 DAOTGZ010000000
VERSION     DAOTGZ010000151.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868088
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 6777)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6777)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:22:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,785
            CDSs (total)                      :: 2,751
            Genes (coding)                    :: 2,732
            CDSs (with protein)               :: 2,732
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 12 of 19
            Pseudo Genes (internal stop)      :: 5 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6777
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13363_length_6777_cov_5.130467"
                     /isolate="AMR_MDS_3064"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..446)
                     /locus_tag="P5123_09780"
     CDS             complement(<1..446)
                     /locus_tag="P5123_09780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002565509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HRX16594.1"
                     /translation="MAKTLLEMKNITKAFTGVIALDDVSLEIEEGEIHALVGENGAGK
                     STLMNVLSGVFPVGSYDGDIEYDGEYCRFNTIRDSEHLGIVIIHQELALVPLLSIGEN
                     LFLGNERGTYSNIDWDETFARADYYLKMVGLQESSRTLIKDISVGK"
     gene            complement(693..1772)
                     /gene="chvE"
                     /locus_tag="P5123_09785"
     CDS             complement(693..1772)
                     /gene="chvE"
                     /locus_tag="P5123_09785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014271575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter substrate-binding protein"
                     /protein_id="HRX16595.1"
                     /translation="MRKKSLIVVLLAASLVLFAGCKQGGSDGVKVGISMPTQSLQRWN
                     QDGANMKAQFEKAGYQVDLQYAGDNDIPTQVAQIENMIATDCKVIVIAAVDGSSLTEV
                     LKQAKQKNIPVIAYDRLIMNSDAVSYYATFDNFKVGTIQGEFLRDALKLESTDGPYNV
                     ELFTGSPDDNNINFFFGGAMSILQPYIDNGKLVVKSGQTSKAQCATPNWSTEEGQKRM
                     ENLITANGYGPTGTKLHAVYSSNDSVAQGITNALIGAGYTKENFPLITGQDCDKTSVK
                     NMLKGQQAMSIFKDTRTLAAQVVKMSDQIIKGQTVDVNDTKTYDNGTGVIPSFLCEPV
                     FATVDNYKKLLIDSGYYTEADLKID"
     gene            complement(2525..3550)
                     /locus_tag="P5123_09790"
     CDS             complement(2525..3550)
                     /locus_tag="P5123_09790"
                     /inference="COORDINATES: protein motif:HMM:NF024823.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HRX16596.1"
                     /translation="MKKTIIALAFVVFFMNGTAEARERITMFNSETETPGWPGVIIGR
                     VLSLGAREIADKELRQLAQEKTKVTVRIDSREGLLPGDELFIIDSKNLIVSRFKVITI
                     NESRSFGYLLVGYGNFRRVQRDFRVAQHSKKALGKNAYIHKARGDYHHANGDVAKAIV
                     EYERSISVNDKYAESHIEIGYIYLEKGIYQYALRAFDKAYKARSFIYDREDLFRLYNG
                     IIKVRSYNAYSLELSNRDIIKRNIEEGIKVAREAVALFPDSFELFYSLGRFYYENPEP
                     HDIDAKNAFLHVLKLQPNHVNSLVMLSELYSKHGNQERAREYAAKAISIDPVNKRARD
                     ILFKYEN"
     gene            complement(3547..4671)
                     /locus_tag="P5123_09795"
     CDS             complement(3547..4671)
                     /locus_tag="P5123_09795"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: protein motif:HMM:NF013700.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M23 family metallopeptidase"
                     /protein_id="HRX16597.1"
                     /translation="MKDKIFMLFITFIVAISGVLQLAAASGKIYIVDGFDSLDGQFRA
                     VEDVLLIDSLEMNSSKSAPEAELSSGEIIYDDNFIDNDEVEAIKKISVKESENSKEKI
                     LKELKKKDYRWHISTHKIKKGENIWNIARAYGVSHKIIISYNSIKNPDRIRPGKELDI
                     PNKRGVEYKVKSGDTLSQIALDYNVSLNTAKKYNKNRDQIKIGQMLFLPDAVQVIEQS
                     ASKNISQVADNKVKTSDNRSRVAAQVSDGIVFGWPLHGDITSHFGMRVHPISGNRQYH
                     NGIDIRADIATAVRSSEKGRVIFCGWKDGYGKMIVLKHEKNYLTVYAHLSGYLVKKNE
                     TVKKGQKIGLSGNTGLSTGPHLHFEIRKYETPLNPIRMIK"
     gene            complement(4709..5851)
                     /gene="hflX"
                     /locus_tag="P5123_09800"
     CDS             complement(4709..5851)
                     /gene="hflX"
                     /locus_tag="P5123_09800"
                     /inference="COORDINATES: protein motif:HMM:TIGR03156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase HflX"
                     /protein_id="HRX16598.1"
                     /translation="MDASVNRIPSSRDEKSFLLSIQLPDISREEALYSLDELKMLVKT
                     AGGSVAGSRLIKRQKYDSSYIAGKGFWEDNVALFEELEIKLVILDVNNLRPGQIRNIE
                     EKIGMRVIGRTEIILDIFACRARSHEAQMQVELAKLGYMLPRLRGYGAVLSRQGGGIG
                     SRGAGESMLEKDKRHIQKRISVLRNNLKKIEKHRELTRKGRHGAIVCAVAGYTNAGKS
                     TLINTLSGDDLFVENRLFATLESYTRTTYIGDGRSILLTDTVGFIRNLPHDLIASFRS
                     TFEEIESADMVLHVVDCCSTDIHTNIKTVLSELERCSDVERPVILYFNKADQISEERR
                     LQLASEYPEGVFGSALDKKNLSELLLSLNRGFEEIASKKYPHYLKV"
     gene            complement(5832..6305)
                     /locus_tag="P5123_09805"
     CDS             complement(5832..6305)
                     /locus_tag="P5123_09805"
                     /inference="COORDINATES: protein motif:HMM:NF014678.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF192 domain-containing protein"
                     /protein_id="HRX16599.1"
                     /translation="MKLRFTILVCMLSILPLVSTVAAGKAQPKLSQCTVTVINNDNKE
                     LKITAEIADTPSARAYGLMFRKELAENEGMLFVFKKDDYLNFWMKNVTLPLSIAFVDS
                     SGKITDLHKMKPLDASISYLSSVPVRYALEVNQGWFKKNNVNKGNRIILHGCISQ"
     gene            complement(6338..>6777)
                     /locus_tag="P5123_09810"
     CDS             complement(6338..>6777)
                     /locus_tag="P5123_09810"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRX16600.1"
                     /translation="TGGSGSEETETAEWDDAITSPEGDKDSFYTYNQMIYGPYLVVTK
                     PIYILDEEQHISFGVRTPVQPEYVPNDTSYFVSLDIPLGGYFALKGEVERLYWNFSRL
                     DQVLFNCGLRYNIFAKVGLELDIIVEKEHGVNRVVRVEYTDEF"
BASE COUNT         2066 a         1455 c         1080 g         2176 t
ORIGIN      
        1 tgttttccaa cacttatgtc tttaatcaat gttcgggaag actcttgcaa gcccaccatt
       61 ttgagataat aatccgccct ggcgaatgtc tcatcccaat caatattact gtatgttcct
      121 ctttcgttgc cgagaaagag attctctcca atagaaagaa gaggtaccag agcaagttcc
      181 tggtgaatga ttacaattcc aaggtgctca ctgtctctga tagtattaaa ccggcaatat
      241 tcgccatcgt actcgatatc accatcatat gacccaacag ggaacactcc gcttaataca
      301 ttcatcaaag ttgattttcc tgcaccattt tcaccaacca gcgcatgaat ttcaccttct
      361 tctatctcaa gactaacatc atctaaagca attactcccg taaaggcctt tgtgatgttc
      421 ttcatctcca acaatgtttt agccaattta accctccatt tccaatacag actaacagaa
      481 aaagacaaac gtataacaga taacccgttc aagactattt ataaataaat ataaaatttc
      541 aatttgcagt cgcagcagac agtacaaaaa acggtaaact atatggcggt aaatcaaaga
      601 tcgaaacagg cattaaattc aaagctaccc ggctgcggcg cggcaacgcc gcagccgggt
      661 aaaatctttt tctgtttaaa cgttactaaa tcttagtcaa ttttaagatc tgcttcagta
      721 tagtaaccgc tgtcgatcag aagtttttta tagttgtcaa ctgttgcaaa aacaggctca
      781 caaaggaatg atggaattac accggttccg ttgtcatatg tttttgtatc attaacatca
      841 acagtttgtc ccttaataat ttggtctgac atcttaacaa cctgagcagc aagagtacga
      901 gtgtctttaa aaattgacat tgcctgctga cctttaagca tgttttttac agatgttttg
      961 tcgcaatcct gaccagtaat caaaggaaag ttttcttttg tgtaacctgc acctataaga
     1021 gcatttgtga taccctgagc tacagagtca tttgaagagt aaacagcatg aagctttgtt
     1081 ccagttggac cgtaaccatt agctgtaata aggttttcca ttctcttctg accttcttca
     1141 gttgaccagt ttggtgtagc acactgggct tttgaagttt gacctgattt aacaacaagt
     1201 tttccgttgt caatataagg ctgtaaaata ctcatagctc caccgaagaa gaaattgatg
     1261 ttattgtcat caggagaacc tgtaaaaagt tctacattgt aaggaccgtc agttgactca
     1321 agtttcaaag catcacgaag aaactcgccc tgaattgttc caactttaaa gttatcaaat
     1381 gtagcgtagt aagaaactgc atcactattc attatcaagc ggtcataagc aataacagga
     1441 atgtttttct gtttagcctg ctttagcacc tctgttaaag atgaaccgtc tacagcagca
     1501 ataacaataa ctttacagtc agttgcaatc atgttttcaa tctgagcaac ctgagttggt
     1561 atgtcattat caccagcata ctgaagatca acctgataac cagctttttc aaactgtgct
     1621 ttcatattgg cgccatcctg attccagcgc tgaagacttt gtgtaggcat ggagataccg
     1681 actttgactc catcagaacc accttgctta cagccggcaa acaataccag cgaagcggct
     1741 agcaaaacca ctattaaact tttttttctc attccgagaa cctcctttgt ttttccgaaa
     1801 ccaagtccgg gttttggtcg acatatttta aaccttatgg taaccaaaat cattttaaat
     1861 gtacataaac tccaattttc caattttctc aagccagaaa atatacacaa taagcaaaac
     1921 cagcaaaaca aatagatatc tcaattaacg caataatcag taatcgcact aaccggtgcc
     1981 atatttcttt gtgataacct gtaacttata ttttgaaaac agatgttatg taccataact
     2041 ataataaaaa taaacgataa cacaatcaaa agtcaataca atattaggcc aaccgttaaa
     2101 ataagtaacc gacaaaaacg tcattacatc taaacacaaa ttattctaaa tttgaaatac
     2161 attgttcgat taaatgcata aaaataacca aaagcatttc tgttatttat actatttttt
     2221 aaaatttgtc aatggaacat ttgaaagcta tatgcacttt ttgaattttc catctcaata
     2281 tgatcatatt agagataatt agcccgtatg gactcaaaaa catacatgca ctttttgaaa
     2341 agcgcattac atttttgagt tttttatcaa aaaccgaccg gccggtttga aatctatatg
     2401 gatcttttga gatttattgt tatttttttt acaattatag attaaattag tttttcttta
     2461 ttaataaaaa aaaatccaaa aaaaatgatt tttttggatt ttttttaata ataacttata
     2521 atttttaatt ttcgtactta aacaaaatat cccgagctcg tttatttacc gggtcaattg
     2581 agattgcttt tgctgcatac tccctggctc tttcttgatt cccatgcttg ctgtacaatt
     2641 ctgacaacat taccaaagaa ttcacatgat tcggctgtaa ttttaataca tgaaggaaag
     2701 cattcttggc atctatatca tgtggttcag ggttctcata gtaaaacctg cctaatgaat
     2761 aaaataactc aaatgaatca ggaaacaaag caacagcttc acgggctacc tttatccctt
     2821 cttcaatatt ccgtttaata atatcacgat tagaaagctc gagagaatat gcattatacg
     2881 atctaacctt tattattccg ttatatagcc tgaaaaggtc ctcgcggtca taaataaaag
     2941 atcgtgcttt atatgccttg tcaaatgccc ttaaagcgta ttgatatata cctttttcaa
     3001 gataaatata tccaatctcg atatgcgact ctgcatactt atcatttaca ctaatagaac
     3061 gctcatattc gacaatagcc tttgcaacat cgccatttgc atgatggtaa tcaccgcggg
     3121 ctttatgaat atatgcattc tttcccaatg ccttttttga atgctgtgcg accctgaaat
     3181 ccctctgaac acgcctaaag tttccatagc ccacaagaag atacccaaaa gaacggcttt
     3241 cattgatagt tataacttta aatcttgata caatcaaatt tttactatct attataaaaa
     3301 gttcatctcc gggaagaagt ccttcccggc tatcaattct cactgtcact tttgtttttt
     3361 cttgtgctaa ttgacgcagc tccttatcgg caatctcccg ggcacctaaa gacagcacac
     3421 gtccgattat cacacccggc cagccgggag tttccgtctc ggaattgaac attgtaattc
     3481 gctcacgggc ttcagctgtt ccattcataa aaaaaaccac aaatgccagc gcaatgattg
     3541 tttttttcat tttatcatcc taatcggatt taaaggagtt tcatattttc ttatctcaaa
     3601 atgcagatga ggtcctgttg ataatccggt atttccgctt aaaccgatct tttgcccttt
     3661 tttaaccgtt tcattttttt taactaaata gccgcttaaa tgagcataaa ctgtaagata
     3721 gtttttttca tgtttcaaaa ctatcatttt cccataaccg tctttccaac cgcaaaaaat
     3781 aacccgtccc ttttctgatg atctgacagc agttgcgatg tctgcacgaa tatcgatacc
     3841 attatgatac tgcctgtttc ctgatattgg atgaacccgc ataccaaaat gtgaagttat
     3901 atctccatgg agaggccatc caaagactat tccgtccgaa acctgagccg ccactcgact
     3961 tcggttgtct gaagtcttta ctttattatc tgcaacttga ctgatattct tacttgcaga
     4021 ttgctcaatt acctgaacag catcaggtaa aaagagcatt tgacctattt ttatctgatc
     4081 tctgttttta ttgtattttt ttgcagtatt taaagataca ttataatcaa gagctatctg
     4141 gcttaatgta tctccgcttt ttaccttata ctcgactcca cgcttatttg gtatgtccaa
     4201 ttctttgccc ggtctgattc tatccggatt cttaatacta ttgtaagata taattatctt
     4261 atggcttaca ccgtaagccc gtgcaatatt ccagatattt tcgccttttt ttattttatg
     4321 cgtacttata tgccatctgt aatccttttt tttcagttct tttaatatct tttctttact
     4381 gttttcgctt tcttttacag agattttttt tattgcttct acttcatcat tatcaataaa
     4441 attatcatca tagataattt cacctgatga taattcagcc tcgggtgcac ttttagacga
     4501 attcatttca agtgaatcaa tcaaaagaac gtcctctaca gcgcgaaact gaccgtcaag
     4561 cgagtcaaac ccgtcaacga tatatatttt accagaggca gccgcaagct gtaaaacacc
     4621 ggaaatagca acaataaagg taataaaaag cataaatatt ttatctttca tgcctttatt
     4681 atcggatatt atgtcatttg gcttaaaact aaactttaag gtaatgagga tatttttttg
     4741 atgctatctc ttcaaaaccc cggtttaacg aaagaagcag ttcggaaaga ttttttttgt
     4801 caagagcaga gccaaacaca ccttcaggat attctgaagc cagctgcaac cttcgctctt
     4861 ctgatatctg atctgcctta ttaaaataga ggataactgg acgctctaca tcactgcatc
     4921 tttccagttc agaaagaaca gtttttatgt ttgtatgtat atctgtggaa cagcaatcaa
     4981 ctacatgcag aaccatatct gcagactcta tctcttcaaa tgtggaacgg aatgatgcta
     5041 ttaagtcgtg tggaagattt cttatgaaac cgacagtatc agtaagaaga atagatcttc
     5101 catctcctat atacgttgtt cgggtataag actccagcgt tgcaaaaagt ctgttttcga
     5161 caaataggtc atcacccgat aatgtattta tgagtgttga tttacctgca ttagtatatc
     5221 ccgccactgc acagacaata gcaccgtgcc ttcccttacg ggtaagttca cgatgttttt
     5281 ctattttttt aaggttgttt cgaagtacag atatgcgctt ttgtatatga cgtttatcct
     5341 tctccagcat cgactcaccg gcacctcgag agcctattcc ccccccctgt cgggacagaa
     5401 cagcgccata gcctcttaat ctgggaagca tataccccaa cttggcgagc tctacctgca
     5461 tctgagcctc atgacttctt gcacggcagg caaaaatatc cagaataatt tctgtgcgac
     5521 cgataacccg cataccgatc ttttcttcga tattccgtat ctgaccagga cgtaaattat
     5581 tgacatcaag aatcacaagc ttgatttcaa gctcttcaaa aagcgcgaca ttatcttccc
     5641 agaagccctt accggcaata tacgaactat catatttttg tcgtttaatt aatcggcttc
     5701 cagcgacaga cccgccggct gttttaacca gcattttaag ttcatccaga gaatacagag
     5761 cctcttctct ggatatatca ggcagctgaa tcgaaagtaa gaaacttttt tcatcacggg
     5821 aggagggaat tctattgact gatgcatcca tgtagtatta ttctgtttcc cttgttaaca
     5881 ttattttttt taaaccaacc ctggttaact tcaagagcat accgtaccgg aacactggac
     5941 aggtatgata tcgaagcatc taaaggtttc atcttgtgca aatcagtaat tttcccggag
     6001 ctatcaacaa aagcgatact caaaggaaga gttacatttt tcatccagaa attaagataa
     6061 tcatctttct tgaatacaaa gagcattccc tcgttttctg ccaattcttt cctgaacatc
     6121 aggccataag cacgcgcgga aggggtatcc gctatttctg cggttatttt gagttcttta
     6181 ttatcattat ttataacagt aaccgtacat tgagaaagct taggctgagc tttccccgca
     6241 gcaacggtag aaaccaaagg aagaattgaa agcatacata ccagtattgt gaatcttaat
     6301 ttcatatctg cttctgcccc cccggtttta accactttta gaattcgtca gtatattcta
     6361 cccttactac tctgttaaca ccatgttctt tttcaacaat aatatcaagt tcaagaccaa
     6421 ctttggcaaa aatattgtaa cgcaatccgc aattaaacaa aacctggtca agccgtgaaa
     6481 aattccagta taatcgttca acctcacctt ttaatgcgaa atatccacct aatggaatgt
     6541 ccagtgaaac aaaatacgat gtatcattgg gaacatattc aggctgaact ggtgttctga
     6601 ctccaaaact gatatgctgc tcttcatcaa gaatatagat tggtttcgta acaacaagat
     6661 atggtccgta aatcatctgg ttgtaagtat aaaaagagtc cttatctcct tcaggactgg
     6721 ttatggcatc atcccattcg gccgtttctg tctcttcaga gccagaacca ccagtct
//
LOCUS       DAOTGZ010000152         6774 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_3064
            NODE_13373_length_6774_cov_5.702485, whole genome shotgun sequence.
ACCESSION   DAOTGZ010000152 DAOTGZ010000000
VERSION     DAOTGZ010000152.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868088
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 6774)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6774)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:22:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,785
            CDSs (total)                      :: 2,751
            Genes (coding)                    :: 2,732
            CDSs (with protein)               :: 2,732
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 12 of 19
            Pseudo Genes (internal stop)      :: 5 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6774
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13373_length_6774_cov_5.702485"
                     /isolate="AMR_MDS_3064"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..436)
                     /locus_tag="P5123_09815"
     CDS             complement(<1..436)
                     /locus_tag="P5123_09815"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRX16601.1"
                     /translation="MNKILRTKFRIITFAFFLTLVNVIFRRLFSIMIFSVGLPPAIHP
                     VASWLLVLIVPWVPRFVFYMPFIIAESFALYMIYYRGVPDNLNAYVGTILNLIMIVLV
                     SETFRYAINRIKTANKKLAIERERADLATKRKDNKYRADAKKK"
     gene            1057..1983
                     /locus_tag="P5123_09820"
     CDS             1057..1983
                     /locus_tag="P5123_09820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015310936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MoxR family ATPase"
                     /protein_id="HRX16602.1"
                     /translation="METIEKIIKNIEQVIIGKRFEIELVIKAIIAEGHILIEDIPGTG
                     KTTLVKALASSIKLNYSRIQFTPDLLPSDITGISVYNRKTDNFEYKKGPVYTNILLAD
                     EINRTSPKTQSALLEVMEEQQISESGNTYTVEKPFTVLATQNPIEYEGTFKLPEAQLD
                     RFLISLTLGYADPAHESRILQSRANSDPLAKITPVADSDTILDLQKKRAEVAVSTEIS
                     NYIVALGIATRDNSQVRLGASTRALIALQKMAQAAALFSGRDYVIPEDVRSHVPYVFG
                     HRVSLSPLSRTDERDKISIIREIADSVKMPGV"
     gene            1985..3124
                     /locus_tag="P5123_09825"
     CDS             1985..3124
                     /locus_tag="P5123_09825"
                     /inference="COORDINATES: protein motif:HMM:NF013998.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF58 domain-containing protein"
                     /protein_id="HRX16603.1"
                     /translation="MISINRAYLLSLVPAGIAAFALLRYPIFFIFFMLLFLGVYSVIL
                     IIVLYKTTIIRFEQPSGILYAGNPVKWSLSADNVFPVPSVKIKLKDRSIPRSLTTMSE
                     GTITSYRNCRIESSSVFPSRGIYNLSHCEILLQDVFKIASCKFTYDAEQKIYIYPALY
                     DTDNLFLAGRDIYRDEKDLLNGRENPEQISEIRGYVHGDSIKKIHWKQSARQDELQVK
                     SFERCAGSSLVILADFNDTNNDFDPSEEEAIADITVSTAYKALKQGISTTVTCNNSLF
                     FNNSYNHYHEFDFFYRSMLTVKSDGKTPFAQFVMDRISGLDRMTQVIAVTLKLDIQKQ
                     KAFDYIINRGNSFAVIYLQPQSNTEAPFYAINGHDIITPDRRIIL"
     gene            3124..5373
                     /locus_tag="P5123_09830"
     CDS             3124..5373
                     /locus_tag="P5123_09830"
                     /inference="COORDINATES: protein motif:HMM:NF013961.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglutaminase-like domain-containing protein"
                     /protein_id="HRX16604.1"
                     /translation="MDSLLARPALHKILIKYLKQKPGQAATAILLLQTVYGLSKIMEI
                     RKGLTASLFFAVLFTYPLLYIFLKKRSDIISSLFFKSVATVSLYLLPAFSVNLFTSGF
                     GGISYFSIFSGIMDKGIHESSEFITLISIATCLLTLIAALLIHLGRDMVAFFIISIPA
                     ILIILNIPEDKAYTISSTVILTTFTLLAFTVNSTRERKNSNHLVYHVTITLLTVFIPV
                     MILTKIFSASGTAALFEDIYKKLDYAKRVQFSQATAGFSEKETELGGPVKTVDKTEFF
                     VNSPVKVKLRGSVKLKYTGTTWETHYASWNTNVTVPAQITYLSNNPAFTRYELTVTPE
                     ESRLTTYLTPLFTQSVESDDIKFSTTGIYKTESEREIEDTYQVVFYHTETDLLEQFTY
                     SPKTEERTLRQMRLKYSDYLLIPKTVTDRTRDLALEIAGTNKNYSEIAKSIEAFLNHN
                     YTYTTDASHLPPDRDFVDFFLFTDRKGYCTYFATAAVILLRINGIPSRYVEGFETQKL
                     QDAKGRYIIKSAMAHAWCEVLIDPQNDIWAVFDPSPSSYYTPSDESYWKHFVYDYLLR
                     TGENVDSLTQDENSANKTVYHLSSPKETVLSRYLPLVYLVIPLLLLFVLTVIIKNLCL
                     ILFSNSLFYFYRFTGSLYQNSKFFDKSSSKTLFEELYSLNNSERFIDTAHTITTEYYT
                     QRFKINKEYKSNVKTRLKLFIVLSLSYFKVSIETPLSAITAALFDLKKTTASGDNRYF
                     KTDLNKQNN"
     gene            5587..6732
                     /locus_tag="P5123_09835"
     CDS             5587..6732
                     /locus_tag="P5123_09835"
                     /inference="COORDINATES: protein motif:HMM:NF012318.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trypsin-like peptidase domain-containing
                     protein"
                     /protein_id="HRX16605.1"
                     /translation="MKAYKKTTISILIIFISSIMLYTFSCKGESGSDFLSGSRPDISD
                     SGALTDATNVQKAFNKIYDLYENTVVFISTEQTIKMRQHPFFNDPFFREFFGGRPMPE
                     EQERKRTGLGTGFILSDDGYIATNHHVVANVDKVEVRIDEKTYEAKIIGSDEATDLAL
                     LKISSSEELTPAFLGDSDKVKVGDWAIAIGNPFGLDRTFTTGVISAVGRNDLDRSGRS
                     SSHLQTDASINPGNSGGPLINIDGQVIGINRMIYSKSGGNMGIGFAIPINTARDILRQ
                     IREKGKVSRPFIGVQIARLTEDYAKKAGLDEPKGALVAEVVKESPAEKGGIRQGDIIV
                     AVNDKKVENFPDLINAVESHSIGTKLNVTVIRDKKRIKVSLIATERP"
BASE COUNT         2172 a         1457 c         1232 g         1913 t
ORIGIN      
        1 ttttcttttt ggcgtcggct ctgtatttgt tatctttgcg tttagtagcc agatcggctc
       61 tctctcgctc gatagcaagt tttttgttgg cagttttgat acgattaatt gcatatctaa
      121 aagtctctga tacaagtaca atcatgataa gattcaaaat tgtaccgaca tacgcattta
      181 agttgtcagg cactcctcta taatagatca tatatagggc aaatgattcc gcaataataa
      241 agggcatata aaagacgaat cgcggcaccc aaggtactat taaaactaga agccaacttg
      301 caacaggatg tatagctggg ggcagaccta cagaaaaaat cattatcgaa aaaagacgtc
      361 taaatatgac atttactaaa gttagaaaaa atgcaaatgt gataatcctg aacttagttc
      421 ttaaaatctt attcacttaa cgtttctcca cccattatga gtatatttaa agccatacac
      481 cgtaccatat ttcaaaagaa ctatattttg tacactaaat attctcgatt agagattata
      541 ctatttatca tactgcttgt taataatgat tatatcacaa atcagcaata attcctaaat
      601 tgctttcgat aaaactattc aagaactaac atctgctaaa accgctgacc atgacagttt
      661 gcataccttg gttcatattg cttattagaa tagttttcca atgtcaaaat cagcaatata
      721 cccttttagc acaaattcat aaaactacaa ttgatgatgc atattaatgt aagctgtgcc
      781 atattttttt caataaaaac taagataatc gccaaaattt actttatact aggctgctta
      841 tataagcgcc tagttaaaaa gttaattatt agatattacc atcacaattt aaaccatgtt
      901 actcactgaa aaattgtaat ctttaaagtt accttatatc gcaattagta ctaaagtagc
      961 agatgaaata agcagttacg cttggcaaca agcgtaattg cattgacatt gcaagagttc
     1021 tactccatta tttgtactca aacaaggaga agccccatgg aaaccattga gaaaatcata
     1081 aaaaatatag aacaggtaat aatcggaaaa cgcttcgaga tagaacttgt tattaaggcg
     1141 attatcgccg agggacatat tcttatcgag gatataccag gtaccggtaa aacaaccctg
     1201 gtcaaggctc tggcctcttc gataaaactg aattacagcc gaatccagtt tacacctgac
     1261 cttctaccat cggatatcac tggaatatcg gtgtataacc gcaaaacaga caacttcgaa
     1321 tataaaaaag gccccgtgta cacaaatatt ttgcttgccg acgagatcaa cagaacatca
     1381 cccaagactc aatcggcact gcttgaggta atggaagagc agcaaatatc tgaaagcggc
     1441 aacacctata cagtcgagaa gccgtttacc gtgctggcaa ctcaaaatcc tatagaatac
     1501 gagggtacct tcaaattacc tgaagcacag cttgaccgtt ttttaataag cctgaccctt
     1561 ggatacgccg acccggctca cgaaagccgc atacttcaaa gccgcgccaa cagcgacccc
     1621 ttggccaaaa tcacccccgt agccgattca gatacaattc ttgatttaca gaaaaaaaga
     1681 gccgaagtag cggtatctac ggaaataagc aattatatag tagctttagg cattgcgaca
     1741 cgcgacaatt cacaggtcag actcggtgcg agtacccgag cactgattgc gctgcaaaag
     1801 atggctcagg ccgcggccct ctttagcgga agggattatg taatacccga agatgtgcgc
     1861 tcgcacgtgc cctatgtctt tggtcaccgc gtaagtctct cacctctctc cagaaccgat
     1921 gagcgcgaca agatttccat aatcagggaa attgccgatt cggtaaaaat gcctggagta
     1981 taggatgatt tccattaata gagcttatct tctttcactt gttccggccg gtatagcagc
     2041 ctttgcttta ttgcgttacc ctatattttt tatatttttt atgcttcttt tcctgggagt
     2101 ctactccgtt atacttataa tagttttata caaaacgacc ataatacggt tcgaacaacc
     2161 ctccggcata ctgtacgcgg gaaatccggt aaagtggagc ctctcggccg ataatgtttt
     2221 tccggtacct tcggtaaaaa tcaaacttaa ggacagatca ataccgcgat ctcttacaac
     2281 catgtccgaa ggtacaatca cgagctatcg aaactgccgt attgagagta gttccgtttt
     2341 cccgtctaga gggatttaca atctgtcaca ttgtgaaatt ttattacagg atgtttttaa
     2401 aatagccagc tgcaaattca catacgatgc ggaacaaaag atttacatat atccggctct
     2461 ttacgataca gataatctct ttttagccgg gcgcgacatt tatcgagatg agaaagacct
     2521 tttaaacgga cgtgaaaatc ctgaacagat atccgaaatt cgcgggtatg ttcatggtga
     2581 ctctataaaa aaaattcact ggaagcagag cgccagacag gacgagctac aggttaaaag
     2641 ttttgaacga tgtgccggaa gctctcttgt aatactggcc gattttaacg atacaaataa
     2701 tgactttgac ccttcagaag aggaagctat tgcagatatt acagtttcca cagcatacaa
     2761 ggctttaaaa cagggaatat ctacaacggt cacttgtaac aattcgctct tctttaataa
     2821 cagctacaat cattaccatg aatttgattt tttttacaga tccatgctta cagtaaaaag
     2881 cgacggcaaa acaccatttg cccaattcgt tatggatcgg atatccggac ttgaccgcat
     2941 gactcaggta atcgcagtaa cactgaaact tgatatacag aaacaaaaag cctttgacta
     3001 cataattaac cgcggaaaca gctttgccgt tatatattta cagccacaat caaatacaga
     3061 ggctcccttc tatgccataa acggacacga catcattacc cctgacagga ggatcatact
     3121 ataatggatt ctcttctagc tcggccggca ctacacaaaa ttttaattaa atatcttaaa
     3181 caaaaaccgg gacaggctgc cacggctatt ctacttttac agactgttta cggcctgtca
     3241 aagattatgg aaatacgcaa agggcttacc gcttcacttt tttttgctgt tctattcaca
     3301 tatcccttac tctatatatt tttgaaaaaa cggtcagaca taatatccag cctatttttc
     3361 aaatcggtgg caacggtatc gctatatctg ctaccggcct tttctgtgaa tctttttaca
     3421 tccggcttcg gaggaataag ctatttttca atattttccg gcataatgga caagggcatt
     3481 cacgaatcat cagaatttat cacattgatc tcgatagcga cctgcctttt gacgcttatt
     3541 gccgccttgc ttatacacct tggtcgtgat atggttgcat tctttataat ttccataccg
     3601 gcaattctca ttatccttaa catccccgaa gacaaggcct ataccatttc gagcacagtt
     3661 atattaacca cattcacatt attggctttt acggtaaaca gcacccgtga aagaaaaaac
     3721 tcaaaccatc ttgtgtatca cgtaaccata acgctgttaa cggtttttat accggtgatg
     3781 atccttacaa aaatcttctc ggcatcaggc actgcagcgc tttttgaaga tatttacaaa
     3841 aaacttgatt acgcaaaaag ggttcagttc tcacaggcaa cagccggttt ctctgagaaa
     3901 gagaccgaac ttggcggccc tgttaaaaca gtcgataaga ccgagttttt tgtgaattcc
     3961 cctgtaaaag tcaaactcag aggaagcgta aaactcaaat atacaggaac aacctgggaa
     4021 acccactacg cctcttggaa cacaaatgtc acagtacctg cacagataac ctatctctca
     4081 aacaacccgg cctttacaag atacgaactt actgtaactc ctgaagagtc acgtttaaca
     4141 acatacctga ctccgctttt tacacaatct gtcgaaagtg atgatattaa atttagtact
     4201 accggcattt acaaaaccga atcggagcgc gaaatcgaag acacctacca ggttgttttt
     4261 taccatactg aaaccgattt gcttgagcaa tttacctact ctccaaaaac cgaagaacga
     4321 accttgagac aaatgcgttt aaaatacagc gactatctgt tgatacccaa aactgttacc
     4381 gaccgaaccc gtgatcttgc acttgaaatt gccggcacaa acaaaaacta ttctgaaata
     4441 gcaaaatcaa ttgaggcttt tttgaatcac aactacacgt acactacaga tgccagccac
     4501 ctgccacctg atcgcgactt tgtagatttt tttcttttta ccgacagaaa aggatactgt
     4561 acctattttg cgaccgcggc cgtgattctt ttaaggataa acggaatacc ctcaaggtat
     4621 gttgaaggct tcgaaacaca gaaattgcag gatgccaaag gacgttatat tataaaatct
     4681 gctatggcgc atgcttggtg cgaagtgtta attgatccgc aaaatgatat ctgggcagtt
     4741 tttgatcctt cgccgtcaag ctattacacc ccttcggacg agtcatactg gaaacatttc
     4801 gtttacgact accttttacg tacaggcgaa aacgtggact cactgacaca agacgaaaac
     4861 tctgcaaata aaacagttta tcatttatcc agcccgaaag agaccgtgtt gagcagatac
     4921 cttcctctgg tatatcttgt tattcctctt ttacttttat tcgttttaac cgttattata
     4981 aaaaatcttt gtctgattct ttttagtaac agcctttttt atttctaccg ttttaccggt
     5041 agcctttatc aaaacagcaa attttttgat aaatcgagtt ccaagactct ttttgaagaa
     5101 ctatattcac ttaataattc agaacgcttt atagatactg cccacacgat aacaacggaa
     5161 tattacaccc aacgtttcaa gataaataaa gagtataaga gtaatgttaa aactcggtta
     5221 aaacttttta tagtattatc actctcttat tttaaggtgt ccattgaaac acccctttcg
     5281 gcaattacgg cggctctttt tgatttaaaa aaaaccactg cttccggcga caacagatac
     5341 tttaaaacag atctaaacaa acaaaacaat taatttgtta aatagcagga ttaacagatt
     5401 ttgattttgt aacttttaaa cctccgaaac ctctgtttaa ttcgatgtat atctggtgtc
     5461 atttacttga tcttgattta ataaatcgtg atctattttc ttaccaaatg ggtaacaaag
     5521 tacaaaacca atatactaaa taaacagaat atgcgataat tacacaattt agcggaggct
     5581 tgactaatga aagcttataa aaaaaccact atatcaatac taataatatt tatctcttca
     5641 ataatgctct atacattttc gtgcaaaggc gaaagcggtt ccgatttttt atctggctcg
     5701 cgacccgata ttagtgattc cggagcacta acagatgcta ccaacgtaca aaaggccttt
     5761 aataaaattt acgatttata cgaaaacact gttgttttca ttagtaccga acaaacaata
     5821 aaaatgagac agcatccttt ttttaacgat ccattttttc gtgaattttt cggcggccgc
     5881 cccatgcccg aagaacagga aagaaaaaga acaggactgg gcacaggctt tatactttcg
     5941 gacgacggtt atatagcgac caaccatcat gtcgtagcta acgttgacaa ggttgaagtg
     6001 cgtatcgatg aaaagacata cgaagcaaaa ataattggaa gcgacgaagc aaccgatctt
     6061 gcgcttctta agatcagctc ttccgaagaa cttacacctg cttttttagg tgattcggac
     6121 aaagttaagg tcggggactg ggctattgcc atcggtaacc cttttggcct agacagaact
     6181 tttacaaccg gcgtaatcag cgccgttggc cgcaacgacc ttgaccgaag tggccgttca
     6241 tcttcgcatt tgcaaacaga cgcttcgatt aatcccggaa actcgggagg ccctctaata
     6301 aatatagacg gtcaggttat cggaataaac cggatgatct actcgaaaag cggtggaaat
     6361 atggggatag gatttgcgat cccgattaat acagcacgcg atattttgcg ccagattcgt
     6421 gaaaagggca aggtgagccg accatttata ggtgtgcaga tagcacgatt aacagaagac
     6481 tatgcaaaaa aagcaggttt agacgaaccg aaaggagctc ttgttgccga agtggtaaag
     6541 gagagcccag ccgaaaaagg cggaattagg caaggtgata ttatagttgc agtgaatgat
     6601 aagaaggtag aaaattttcc cgacctgatt aacgctgttg agtcacactc tattggaaca
     6661 aaactcaatg tcaccgttat cagggataaa aagcgaataa aggtttcact gattgctacg
     6721 gagcgcccat agggcgctct tccctctcta cttattgcag actaatgtgt gaac
//
LOCUS       DAOTGZ010000153         6749 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_3064
            NODE_13439_length_6749_cov_5.352256, whole genome shotgun sequence.
ACCESSION   DAOTGZ010000153 DAOTGZ010000000
VERSION     DAOTGZ010000153.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868088
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 6749)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6749)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:22:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,785
            CDSs (total)                      :: 2,751
            Genes (coding)                    :: 2,732
            CDSs (with protein)               :: 2,732
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 12 of 19
            Pseudo Genes (internal stop)      :: 5 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6749
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13439_length_6749_cov_5.352256"
                     /isolate="AMR_MDS_3064"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(235..1266)
                     /locus_tag="P5123_09840"
     CDS             complement(235..1266)
                     /locus_tag="P5123_09840"
                     /inference="COORDINATES: protein motif:HMM:NF012655.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HRX16606.1"
                     /translation="MKEQKKKIDFYKEIGQYFRADGSFDPASFSDVFERHLDRFRSED
                     PDNFKVSFSVWIESYISGIADYLKSKKMARASYELIRYTVDKCVALGIDTISIPAEIL
                     EELFSRSPKSGVESGDDRKKRVSAVEKRSSIFSAALQVFSEMGFHKATMDTIAAIAGM
                     GKGSLYRMFASKDELLEQLLLEEYRKIVQRFSAIFSPTRDIIEEIQEMIEFWVTYIND
                     NPVVYRLIQNTDLRPDVSEKAHFYQYVAENLPMLKERIVSLNKEHKVKYLNFESVLFG
                     IFGFIDGIVHKWISYKMEYPLIKEIPVILECVFNGFLSEEYPRKHFCRSITDNSGEGY
                     SAQDFAEQH"
     gene            1530..5240
                     /locus_tag="P5123_09845"
     CDS             1530..5240
                     /locus_tag="P5123_09845"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HRX16607.1"
                     /translation="MTDGIDNCGKIYQSLMDGSDDALAIFSEDIEYIYANLKWKELFN
                     LPDKIPIGAKFEKKYFKFDSSPIERAITLALAGKPTLDTVQELRSSDGLLTINLSAIS
                     TNSDSSKQVAVKINLPHVSQNRMGKACSILLEYLNLAESIFVQLDKSLGIELINEAGS
                     TLLGKKCDELIGRDWIKTAVSEDYRETCLHMFKELFEGNISALRTIEYPVISSDNSEN
                     TIQWKHSLLKDSKGNTVSILANGINITPAKKTENRLKDSEERFKALAESIDDILLSVN
                     HSRHIIYWNPVSQNLSGISSNDAEGKELEKVVPEFADQGLVEPLMQTIKASKTHHLIK
                     DVTFDNIVYTMDISFFPNRQGATILARNISSQIRFQEQIFEQAQILDQLSEAVITTNS
                     KGTIVTANNPCSEIFNKEMFEILGTNVTSLFTQQSQGRIQSIFENLSEENDFASDEFK
                     IKTGTDATFPALVHISIKKSIKNEATGFILSIIDISETAAIRQEMLLLNTNLEKTVSI
                     RTRELEEAKQQAEIATRAKTDFLANMSHEVRTPMNTIVGLNQMLENSNLTAKQRKYTQ
                     QINESSNQLLKIINSILDYSRLESGTASFDYHDFSMANLLQQLKTKIIEMVGSKKITP
                     LFIISPSFPATIHSDPGRFQQVIEILIDNAVKFTESGEIILAASDTRISDSETELRIS
                     IQDTGIGIGEEMNQQVFTPFTQADTSKTRKYSGAGIGLALCRQIVSKMGGNITFRSTP
                     GIGTTFTITLKIKKNNSTAQKQNLTDLPQKPILVLESREQYIDSITAILSASDTNADY
                     FTDFGSITDKNYKVLITNMETITTSYSIIKERFISKNLPVICYKAFSQYIPAKFTHGI
                     YYLNLPILQSDLLPLLNRVLSGNTVVSPSNNSIAENSIENISGTKILIVDDNKINSEI
                     LTELLTTHGLKAETVFNGFEALKKLETEIFDIVLLDIKMPLMNGFETIEKIRNEKKLF
                     SLPVLALSADTSPSDVETYLKKGFNGYVKKPFDLNTIFKTLSKWINPGDNQITFTDSH
                     QNNNPLKSIDGINIRKLINKINPSPDKLIEIYKMFYTNNHDKIDKLKALHRNKNREEL
                     ITECHNLLSVSGNIEATDLYNAVKKYKISLCNEDYSKLNFLFADLCFQLDRVLNSIKN
                     YLKPDKESVKISEKGKGKPLPQEIEQLKTYLDNSDTRALVYIDELLKNRYEPTIKVLL
                     SNIKNYCLKFDFDTAVEYVNRNTVIFAGEADG"
     gene            5233..6276
                     /locus_tag="P5123_09850"
     CDS             5233..6276
                     /locus_tag="P5123_09850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006878548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HRX16608.1"
                     /translation="MDDSRSTVLIVDDVPENISILNEILHKDHIVKAAINGSKAIEIA
                     TSKNPPDIILLDIMMPEMDGYEVCRILKENPKTKHIPIIFITALDDTLDEERGLSLGA
                     VDYIKKPVNPSIVKRRIQTHLMLFDQNKILEKRIHERTLELEETRLAIVQRLGRAAEF
                     KDNETGMHVIRVSNYCKHMALSDGVAPESAEILLHAAPMHDIGKIGIPDNILLKPGKL
                     TPEEWKIMMTHPRIGADIIGIHDSEILKAAYTCALYHHEKFDGSGYPNGLKGVNIPYF
                     ARVIAIADVFDALTGERPYKRAWSIEEAVNHINEGSGTHFDPSLVDIFNREIDTILDI
                     KKSYPELAVATES"
     gene            complement(6293..>6749)
                     /locus_tag="P5123_09855"
     CDS             complement(6293..>6749)
                     /locus_tag="P5123_09855"
                     /inference="COORDINATES: protein motif:HMM:NF024682.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="phosphohydrolase"
                     /protein_id="HRX16609.1"
                     /translation="IKHSSIPREISKVLGQNNDQITETLIGDLISSSYGKEYLGFSME
                     IFSALCKLKEFNYKTIYFNPLIKSESKKIHRMFHLLFDLYFESLIKEDESSDIFIYFL
                     KDRKNDYSKKTDPARVVIDFMASMTDSFIVNQFNKLFFPKNFGYSILSE"
BASE COUNT         2319 a         1424 c         1180 g         1826 t
ORIGIN      
        1 ctccatatac tcggcactga aaacaccgga cggattataa accgccaaag gtagtcggga
       61 atccaaggtt cttgaaatca ttaattatct ccttaaaaat cattatatac atatttgttg
      121 cgtatcagaa aatcacgata ttctctggaa ccgaaaatcg aatttatcaa aaacgcaaat
      181 ttatctgcgc aaataaaatg cgcttaaaca ggataaacct gtttataaac ctgtttaatg
      241 ctgctcagca aaatcttgcg ccgaataacc ttcaccgctg ttgtctgtaa tacttctgca
      301 aaaatgtttc cgcggatact cttccgaaag aaacccgtta aaaacacact ctaggataac
      361 cggaatctct ttaataagag gatactccat tttatacgat atccacttat gaacaatacc
      421 atcaataaat ccgaagattc cgaatagcac actctcaaaa ttcaaatatt ttactttatg
      481 ttccttatta agcgaaacta tacgctcttt aagcattgga aggttttctg ctacatactg
      541 ataaaaatgg gctttttccg aaacatcggg tctcaaatca gtattctgaa tcaatcggta
      601 aacaaccgga ttatcgttaa tataggtgac ccagaactct atcatctcct gaatctcttc
      661 aataatatcc cttgtgggtg aaaaaatggc gctaaaacgc tgaactatct ttctgtactc
      721 ttcaagcaaa agctgctcta aaagctcatc cttggacgcg aacatacggt aaagtgaccc
      781 cttgcccata cccgctattg cggctattgt atccatggtt gccttatgaa aacccatttc
      841 agaaaatacc tgtaaagcag ccgagaagat actgctgcgt ttttcgacag cagagaccct
      901 ttttttccgg tcatcaccgc tctctacacc cgattttgga gagcgagaga aaagctcttc
      961 aagaatttca gctggaatcg aaatggtatc aatgccgagt gcgacacatt tatcaacagt
     1021 gtaccttata agctcgtatg aagcccgagc catcttctta ctcttaagat aatcggctat
     1081 accgcttata tacgactcga tccagaccga aaaggagacc ttgaaattat cgggatcttc
     1141 acttctgaac ctgtccagat gtcgttcgaa gacatctgaa aacgaagcgg gatcaaagga
     1201 accatcggct ctaaaatact gaccgatctc cttatagaaa tctatttttt tcttctgttc
     1261 tttcataatc acataccgga ctgaccagtc cgtccagatc acatagtttg cagtgtctgt
     1321 caaataaaaa agctaaaaaa cagattaata gagctattta ccgacataat ttgacattgt
     1381 gtcatttttt atccgtaaaa ctgatatata aataaaccga ataaaacgca attatttgaa
     1441 atattgcgct ttatccgcta tactatagta gatatctttt taagataatt ttcgatcagg
     1501 taagcataat gctcaggaca aggtaaacca tgactgacgg catagataat tgtggcaaaa
     1561 tataccaatc acttatggac ggatcggatg acgctctggc catcttttca gaagatattg
     1621 aatacatcta tgcgaacctg aaatggaaag agctctttaa cttgccagat aaaataccaa
     1681 tcggagcaaa atttgaaaaa aaatatttca aattcgacag ctcacctata gaaagagcaa
     1741 tcacactcgc cttagcaggg aaaccgacac tcgatacggt tcaagagtta agatcaagcg
     1801 atggtctttt aacaattaat cttagcgcga tatccacaaa ttcggattca tcaaaacagg
     1861 ttgcagtaaa gataaacctg ccccatgtct ctcagaacag gatgggtaag gcctgttcga
     1921 tacttttgga atatctgaat cttgccgaaa gcatctttgt tcaactggac aaatcgctcg
     1981 gtatagagct tattaacgaa gcgggttcta cactgcttgg aaaaaaatgc gatgagctaa
     2041 taggccgcga ctggataaaa acagcagtct cggaagatta ccgtgaaacc tgtttgcaca
     2101 tgttcaaaga actctttgag ggcaatataa gtgctcttcg tactattgag tatccggtta
     2161 tctcatcaga caatagcgaa aacaccatac aatggaaaca ttcactttta aaagatagca
     2221 aagggaacac cgtatctata ctcgccaacg gcatcaatat aacccctgcc aaaaaaacag
     2281 aaaaccgcct aaaagacagc gaagaacgtt tcaaagcact agccgaaagc attgatgata
     2341 ttctgttaag tgtgaatcac tcacgccata ttatttattg gaacccagtt tctcaaaatc
     2401 tttcgggtat cagctcgaac gatgcggaag gaaaagaact tgaaaaggta gttcccgaat
     2461 tcgccgacca gggtttagtg gaacccctta tgcagaccat caaggctagc aaaacccatc
     2521 atctgataaa agatgttacc tttgacaata ttgtctatac aatggatatc agttttttcc
     2581 ctaacaggca gggggcaaca atacttgcgc gcaatatttc atcacagata cgttttcagg
     2641 agcagatatt tgaacaggct cagatacttg atcagctaag cgaagccgtt ataacaacta
     2701 acagcaaagg taccattgta accgccaaca atccatgttc agaaatattc aataaagaga
     2761 tgttcgaaat tcttggcaca aatgtgacat cacttttcac acagcaatca caaggacgta
     2821 tacagtcaat atttgaaaac ctttccgagg aaaatgactt tgcctctgat gaattcaaaa
     2881 ttaaaactgg tactgatgcg acattcccag ctcttgtgca tatatccata aaaaagagca
     2941 ttaaaaacga agctaccgga ttcattctct cgataattga tatttctgaa acagccgcaa
     3001 tacgtcaaga gatgctttta ctcaatacca acctagaaaa aaccgtatca ataagaaccc
     3061 gggagcttga agaagcaaaa cagcaagccg aaattgcgac aagagcaaag acagactttc
     3121 tggcaaacat gagccacgaa gtgcgcaccc ccatgaatac gattgttggc ctgaaccaga
     3181 tgctggaaaa tagtaacctt acagcaaaac aacgaaaata tacacaacag atcaatgaat
     3241 catcaaacca gcttttgaaa atcattaaca gcatactcga ctattctcgc cttgaatcgg
     3301 gcacagccag ctttgattac catgattttt caatggcaaa ccttttgcaa cagctgaaaa
     3361 caaaaatcat tgaaatggtc gggtcaaaaa aaattacacc actttttatt atatcaccct
     3421 cttttcctgc tactattcac agcgatccgg gtcgttttca acaggtaata gaaatattaa
     3481 tagataatgc tgttaaattt accgaatccg gagagataat tttagcagca tcagatacca
     3541 gaatttctga cagcgaaaca gaactcagaa tatcaataca ggatactgga atcggtatag
     3601 gagaagagat gaatcagcag gtttttactc cttttactca ggccgatacc tcaaaaaccc
     3661 gtaaatacag cggcgccggt ataggacttg ctctttgtag gcaaattgta tccaagatgg
     3721 gtggcaacat tacctttaga agtactcccg gtataggtac aaccttcact attaccttaa
     3781 aaatcaaaaa aaacaactct accgcacaaa agcaaaattt aactgatcta ccacaaaagc
     3841 ctattctggt cctggaatcc agagaacaat atattgactc tattacagcc attctgtcgg
     3901 ccagtgacac aaatgcggat tactttacag atttcggttc cattacggat aagaactaca
     3961 aggttctgat aaccaatatg gagaccatca ccacttcgta ctcaattatt aaggaaagat
     4021 ttataagcaa gaaccttccg gttatctgtt acaaagcttt cagccaatac atacctgcca
     4081 agtttacaca tggaatctat tatcttaatc ttccaatatt gcagtccgac ttattaccct
     4141 tgttaaaccg tgttctttcc ggaaacacgg tagttagccc gtccaacaac tctattgccg
     4201 aaaattctat tgaaaatatt agcggtacca aaattcttat tgtcgatgat aataaaataa
     4261 atagtgaaat actgactgaa cttcttacaa ctcatggact gaaagccgag accgtcttca
     4321 acggttttga ggccttaaaa aaattggaaa ccgaaatttt tgacattgta cttcttgata
     4381 ttaaaatgcc ccttatgaac ggttttgaaa caatcgaaaa aattcgaaac gagaaaaagc
     4441 tcttttcact acctgtactt gcgctatctg ccgacacatc accatctgat gttgaaactt
     4501 atctaaaaaa aggttttaat ggttatgtaa aaaaaccgtt tgatttaaat acgattttta
     4561 aaaccctttc aaagtggata aatcccggcg acaaccagat cacatttacg gattcacatc
     4621 agaacaataa cccactaaaa tcaatcgatg gtataaacat aagaaagctt ataaacaaaa
     4681 taaatccatc tcccgataaa cttatcgaaa tttacaagat gttttatacc aataaccatg
     4741 ataaaatcga caaacttaaa gcccttcacc ggaacaaaaa cagggaagag ctgataacag
     4801 aatgccacaa cctgctctct gtttctggaa atatcgaagc tactgatctg tacaacgctg
     4861 taaaaaaata caaaatatca ttatgcaacg aagattactc aaaactcaac tttctgttcg
     4921 ccgacctctg ttttcagcta gacagagttc taaactcgat taaaaactac ttaaaacccg
     4981 ataaagaaag cgttaaaatt tccgaaaagg gcaaaggaaa accgctacca caagaaattg
     5041 agcaactaaa aacatatctg gataacagcg acactcgagc gctggtttat atagatgaac
     5101 tgttaaaaaa ccgatacgaa ccgaccataa aagttttact ctcaaatatc aagaattatt
     5161 gtctaaaatt tgactttgac accgccgttg aatatgttaa tagaaatacg gtaatttttg
     5221 cgggggaagc cgatggatga cagcagatca acagttctta ttgtagatga tgttccggag
     5281 aatataagta tattaaacga aattcttcac aaggatcata ttgtaaaggc tgcgattaat
     5341 ggatccaaag ctatagaaat tgcgacatca aagaatccgc ccgacattat ccttctggat
     5401 attatgatgc ccgaaatgga cggttacgaa gtatgcagaa tactgaagga aaacccgaaa
     5461 acaaagcata ttcccataat tttcattaca gcacttgatg acacccttga cgaagaaaga
     5521 ggattaagtc tgggtgctgt tgactatatc aagaaacccg taaacccctc aattgttaaa
     5581 agacgcattc aaactcacct tatgcttttt gatcaaaaca aaatactgga aaaacggatt
     5641 cacgaaagaa ctctcgaact tgaagagaca agactcgcta tagttcaaag actggggcga
     5701 gccgctgaat ttaaagataa tgagaccggc atgcatgtca tccgggtcag taactactgc
     5761 aaacatatgg ctctttctga cggcgttgcc ccagaatcgg cagaaattct gcttcacgca
     5821 gcccccatgc atgacatagg aaaaataggt attccggata atatacttct taaacctggg
     5881 aaattgactc ccgaagagtg gaaaatcatg atgactcacc cccgtatagg cgcggatatt
     5941 attggcattc acgattccga aatattaaaa gcagcctaca catgcgcgct ttatcaccat
     6001 gaaaagtttg acggcagcgg atatcccaac gggctaaaag gtgtaaatat tccctatttc
     6061 gccagagtaa ttgcgattgc cgacgttttt gatgccctta ctggtgagcg cccttacaaa
     6121 agagcctggt caattgaaga agcggtaaat catattaacg aaggatcagg cactcatttt
     6181 gatccctcac ttgtggatat ttttaaccgt gagattgata ccatacttga tataaaaaag
     6241 agctacccag aacttgctgt tgcaacagaa tcttgaccat aattagtgat gattattctg
     6301 acaatatgga ataaccgaaa tttttaggaa aaaacagttt gttaaactgg ttaacaataa
     6361 aactatcagt cattgaagcc ataaagtcga taacgaccct agcaggatca gtttttttgc
     6421 tgtaatcgtt tttacgatct ttcaaaaaat agatgaaaat atcagacgat tcatcttctt
     6481 ttattaatga ctcaaaatag aggtcaaaaa gcagatgaaa catgcggtgt atttttttac
     6541 tctcgctttt tatcaaaggg ttaaagtata tcgttttgta attaaactct ttcagtttac
     6601 acagtgctga gaatatttcc atactaaacc ccagatactc ctttccgtaa ctgctcgata
     6661 tcagatcgcc tatcaaggtt tcggtaatct gatcattgtt ttgccccaga accttgctta
     6721 tctcacgggg tattgatgag tgttttatc
//
LOCUS       DAOTGZ010000154         6666 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_3064
            NODE_13631_length_6666_cov_6.177583, whole genome shotgun sequence.
ACCESSION   DAOTGZ010000154 DAOTGZ010000000
VERSION     DAOTGZ010000154.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868088
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 6666)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6666)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:22:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,785
            CDSs (total)                      :: 2,751
            Genes (coding)                    :: 2,732
            CDSs (with protein)               :: 2,732
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 12 of 19
            Pseudo Genes (internal stop)      :: 5 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6666
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13631_length_6666_cov_6.177583"
                     /isolate="AMR_MDS_3064"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..386)
                     /locus_tag="P5123_09860"
     CDS             complement(<1..386)
                     /locus_tag="P5123_09860"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRX16610.1"
                     /translation="MNRITVGYLTFDLAGTYNHQMWHYLSLEAERRGVRLVTFCGGIL
                     YGKSNHQHEWNKIYDLASKESLDGLIVLSASLGQACSTGEMESFLNRFKPLPMVSIGA
                     ALRGIPSVVVNNEAGMARIVEHMIDA"
     gene            complement(550..1143)
                     /locus_tag="P5123_09865"
     CDS             complement(550..1143)
                     /locus_tag="P5123_09865"
                     /inference="COORDINATES: protein motif:HMM:NF014471.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015109 - chromate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015703 - chromate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chromate transporter"
                     /protein_id="HRX16611.1"
                     /translation="MIYLLLFWTFFKIGAFSFGGGLAMIAPVQAEMEYYGWMSADEFA
                     NIIAVSQITPGPIAVNTATYVGIKTAGFMGGLCATLGVSMPALIIMTLIGHFYKQYKD
                     SVLFISVLTLLKPVTLALIASAVVIVARTAFVSSNDYIVVPESINLGIDLVSIILSNF
                     NLIPIIFCLVMIPLVLKFKLHPVLVIAISAVFGLIFL"
     gene            complement(1140..1700)
                     /locus_tag="P5123_09870"
     CDS             complement(1140..1700)
                     /locus_tag="P5123_09870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010251399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015109 - chromate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015703 - chromate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chromate transporter"
                     /protein_id="HRX16612.1"
                     /translation="MNIGINALFDIFITFFKIGSFTIGGGLAMIPLIERETVQNKGWI
                     KEEEISDVISLCQSVPGVIAINISIFIGYRRAGLIGAFFAALGVILPSFLIIMLLSNI
                     LLKNYSNPYVQKIFAGAKVGVTALLAAVLIKLSRQIIKDVYSFIVAFCAFAAVIVFDV
                     HPFIVIGFGIILGIIYYNVFIRGKVK"
     gene            complement(1712..2491)
                     /locus_tag="P5123_09875"
     CDS             complement(1712..2491)
                     /locus_tag="P5123_09875"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRX16613.1"
                     /translation="MITISKHVTGFIEKIFKISLFCMVLTALSQNAFAQAFIDAEYAM
                     VFTGYNDVQVPSDDGTRFSLKDDIASDTGFAPRMRLGYTFLQRHSLLLLAAPLTIYGE
                     GIIDKDISYRGKTFEAGTKVKSTYRFDSYRITYRYTFINSNQLSLAAGITGKMRDAEI
                     RLIGEDGSAGKSNIGFVPLIHLMAQWKFTDKYSVLLDMDALVTPFGRAEDALFALQYH
                     YNEKTSFRLGYRILEGGSDGGGDVYTFALLNYITAGLTTEL"
     gene            complement(3109..4023)
                     /locus_tag="P5123_09880"
     CDS             complement(3109..4023)
                     /locus_tag="P5123_09880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013388583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF523 domain-containing protein"
                     /protein_id="HRX16614.1"
                     /translation="MNNFLSEKINIGISACQFGSKIRYNGKGQDITGFMGREKSDFIW
                     HPVCPEVLSGMGVPRNPVSLRGGNGDDFWEGTADIKNRGGTNLNDWMKKGVESAVGVL
                     KRANVKVYIFMEGSPTCGVYRTSLKNKRLGHPPGIFGSLLLKEGFFLIPSSDLQSALR
                     WWDWRRRLFAFLWIEDQSFDKISSAVDAWHIVKFLVQELSRKEADIIGKTLAQGKSIG
                     KKELLALKREIMDILRRPSTTARIKQSLWKNYTFMRKHFGMSMDNIKMPTELRGFTHI
                     AEELKSLEIDSRKKGYLFGASPVYYKIN"
     gene            complement(4170..4925)
                     /locus_tag="P5123_09885"
     CDS             complement(4170..4925)
                     /locus_tag="P5123_09885"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRX16615.1"
                     /translation="MRQTKGESFYNLLEFVLTMDENPSISGNDKAIFEHLMIEFFFKH
                     ECEEKSILAIFKNMELPAQFKGLKSLLYMPIDSVSDFVNADTINDTFAGRVMLSRRYL
                     KCVYPLQEPEFKMLPVEIQLPLIDRIKDKNKNIIKAFEKMHKDIEDDRNRTVMKLIAL
                     VIKNVHLKTGFPLKDTKRPAVEIIKENYNGYAQIFNGDPDVVVDLTDDKKIRLLAKEF
                     FLVHSHKDIVEISDLLKKEFKRFVWRISKIMQI"
     gene            complement(4928..5926)
                     /gene="tsaD"
                     /locus_tag="P5123_09890"
     CDS             complement(4928..5926)
                     /gene="tsaD"
                     /locus_tag="P5123_09890"
                     /EC_number="2.3.1.234"
                     /inference="COORDINATES: protein motif:HMM:TIGR03723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     transferase subunit TsaD"
                     /protein_id="HRX16616.1"
                     /translation="MKNIIGLGLESSCDETAAAIVENGSRVLSNRIASQIDLHSEYYG
                     VVPEIASRAHLEMINPLIDSALKESGLTFDDIDYVAATNRPGLIGSLMIGLQAAKTIS
                     YIKKIPLIATNHLEAHLYAPFLNSETISYPYIGLLVSGGNTALYLMKGIGEMMLLGRT
                     MDDAIGEAFDKVSKFMELGYPGGPLIDKLARKAVKKEKLFPKLLAAKNEDYRFSYSGL
                     KTAVINYVKNNPEYDINEVAYSFQESAIEILIRRTFAAARDYGLKNIVIAGGVAANSR
                     LRELFLSECRDGEKIIIPPIDLCTDNAAMIAGVSYHYFCRDQFDTLEADVTAKIRV"
     gene            complement(5923..>6666)
                     /locus_tag="P5123_09895"
     CDS             complement(5923..>6666)
                     /locus_tag="P5123_09895"
                     /inference="COORDINATES: protein motif:HMM:NF015531.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S41 family peptidase"
                     /protein_id="HRX16617.1"
                     /translation="NFYSDTHLDVRKALLDLEKKGVKSLILDLRNNPGGNLETSIHIA
                     DYFLPKNSVIVSTRGKEGSGVSEEYKAEHDVLFKGDILILVNEGSASASEVLSGALQD
                     NKRAILAGETTFGKALVQRVIDIEKGKTGFALTIRKYYTPSGKMIHKAGIKPDYLVKQ
                     DIIPENDKKNLGRVFNDKLIEKFAQKYEKYNEENIELLVSFLKSKNVPLSKKVAAFYY
                     KFELNRDKPDALYDLEFDNQLKDSLKYLK"
BASE COUNT         2161 a         1445 c         1066 g         1994 t
ORIGIN      
        1 tgagcatcaa tcatatgttc gactatccga gccatacctg cttcattatt taccacaaca
       61 gaagggatac ctcgtagcgc ggctcctata ctaaccatcg gtagaggttt aaaccggttt
      121 agaaaagatt ccatttcacc tgtactgcaa gcctgcccca gagaggcact taacacaatc
      181 aaaccgtcaa gagattcttt agacgcaaga tcatatatct tattccattc atgctgatgg
      241 ttactcttgc cataaagaat accaccacaa aaagtaacca gccttactcc acgacgctct
      301 gcttcaagcg acaaataatg ccacatctga tggttatatg ttccagcaag atcaaatgtt
      361 aaatacccga ctgtaatacg attcaatggc caactccttg ctcaatacat aacagtatat
      421 acctttttta agaattaatc aaaataatat tacaccaaac agttatgctt tctgcaacaa
      481 gatgattttc aagtttccta tgaatcgtta aagatgaatt agtaaaatag gggcgatatt
      541 gacgtcatat tataaaaaaa tcaggccaaa aacggctgat atcgcaataa ccagaacagg
      601 atgcagtttg aattttaata cgagagggat catgaccaga caaaatatta tgggtatcag
      661 gttaaaattc gataaaatta tactaacaag atcaatacct aaattaatag attctggtac
      721 tacgatgtaa tcattactgc tgacaaaagc tgttcgtgca acaataacaa ccgccgaagc
      781 aatcaaagcc aaagtaacag gctttaaaag agttaaaaca gaaatgaata gaacgctatc
      841 tttgtactgc ttataaaaat gccctatcaa cgtcatgatt atcaaagccg gcatcgaaac
      901 acccaaggta gcgcataatc cccccataaa tccggctgtt ttaattccaa cataggtagc
      961 tgtattaacc gcaataggcc ctggggttat ctgcgaaacc gcgataatat tggcaaactc
     1021 gtccgcgctc atccagccat aatattccat ttcagcctga accggagcta tcatggccag
     1081 acctccgcca aagctaaacg cgccaatctt aaaaaaagtc cagaataaca acaaatatat
     1141 catttaactt taccgcgaat aaatacatta taatagatta ttcctaaaat tattccaaac
     1201 ccaattacaa tgaaaggatg aacatcaaag actattacag ccgcaaaagc acaaaaggct
     1261 actataaaag aataaacatc cttgattatt tgtctactca gcttaataag aaccgcagct
     1321 aatagtgcag taacccccac cttggcaccg gcaaagattt tctgaacata aggattagaa
     1381 tagtttttca gaagaatatt agataaaagc ataattatta gaaacgaagg aagaataact
     1441 ccaagcgccg caaaaaacgc accgattaac cctgcccgtc tataacctat aaaaatggaa
     1501 atattaatag caataactcc aggtaccgat tgacaaaggg aaataacatc agaaatttct
     1561 tcctctttta tccacccctt gttttgaaca gtctctcttt ctatcagtgg aatcattgct
     1621 aaaccaccac caatagtaaa cgaacctatt ttaaaaaatg ttataaaaat atcaaaaaga
     1681 gcgttaatac ctatattcat tgcaatcaca cttataactc tgtagtcaaa cctgctgtga
     1741 tataatttag cagagcaaaa gtataaacat ctccaccgcc atccgagccg ccttcaagta
     1801 ttcgataacc cagtctaaaa cttgtttttt cattatagtg atactgtaat gcaaaaagcg
     1861 cgtcttcagc ccttccgaaa ggtgtaacaa gagcatccat atcaagtaaa acactgtatt
     1921 tatccgtgaa tttccactga gccataagat gtataagtgg cacaaaaccg atattgcttt
     1981 tgccagcgga gccgtcctcg cctatcaacc ttatttcggc atcgcgcatt ttcccagtta
     2041 tacctgcggc aagtgaaagt tgattactat ttataaaagt ataccggtaa gtaattctgt
     2101 aagagtcaaa tctataagtt gatttcacct tagtaccggc ttcaaaggtc ttcccacgat
     2161 aagatatatc tttatcaatg attccttctc cataaatagt caatggtgcc gcaagaagaa
     2221 gcaaactatg cctttgtaaa aaagtataac caagccgcat tcttggcgca aaaccagtat
     2281 ccgaggcaat atcgtctttt aacgaaaaac gtgtgccatc atcagaagga acctgtacat
     2341 cattgtatcc ggtaaaaacc atagcatatt ctgcatctat aaatgcctga gcaaatgcgt
     2401 tttgactaag cgcagtcaaa accatacaaa acagacttat tttaaaaatc ttttcaataa
     2461 aaccggttac atgttttgaa attgttatca cattctatct ccatcttgac ccggacttaa
     2521 tactccatca ttatatacac acataagctg aatatatcaa ctattttata tccggataac
     2581 attaacatat tcacctatca gcaaattccc cggtataatt aagaaatacc ggttatttcc
     2641 ataattaatt ttccagcata atcaaatact atatatttat atatcagtca acggtcaaac
     2701 caggttaaaa tttcaaagca aattgataaa aacacatatt aatgacttaa acatcattta
     2761 tatattttaa accgacttta taaaccgtga ggattgactt tccatgtaga aatttataat
     2821 cagtattgca ttaaaggatc accatgcagt attgttgagg tgcgttgaaa gtgaaggttt
     2881 ttccagctgg aaaagcatta ttagcaaacc gatatccttg gccgaataag gtactgacac
     2941 ctgtaaaacc aagaacaaag ccgtgttcac aaaccctgaa tcatatgcta tcacccaact
     3001 atgaagcgaa aaatatttta atcagcggta attacagaga agtgaggagc acattttata
     3061 cagttcagtc atactaaaaa gaaccgttta cctaaagtat gcggttcttt agttaatttt
     3121 atagtaaaca ggtgacgcgc caaaaagata gccctttttg cggctgtcta tttcaagtga
     3181 tttcagctct tccgcaatgt gtgtaaatcc gcgcagctca gtcggcattt tgatattatc
     3241 catggacatg ccgaaatgtt ttcgcataaa tgtataattt ttccataatg attgtttgat
     3301 tcgcgctgtt gtagaaggcc ttcgcaaaat atccattatc tctcttttaa gagctaaaag
     3361 ttctttttta ccgatagatt ttccctgtgc cagagtcttg ccgataatat cggcctcctt
     3421 tcgcgacagc tcctgcacaa gaaatttgac tatatgccag gcatccacag cactggatat
     3481 cttatcaaaa gattgatcct caatccataa aaaagcgaac aaacggcgtc tccagtccca
     3541 ccatcgcaat gcactctgaa gatcgcttga tggtattaga aaaaagcctt ccttcaataa
     3601 aagcgaacca aatatgccag gtgggtgtcc cagcctttta ttttttaaag aggtacggta
     3661 aacaccgcag gttggacttc cttccataaa gatataaacc ttaacattgg ctctctttaa
     3721 tacaccaacc gccgactcca cacccttttt catccaatcg ttaagattag tgccgcctct
     3781 gtttttaata tccgctgttc cttcccagaa atcatcaccg ttaccgcccc gcagacttac
     3841 cgggttacga gggacgccca tcccgctcag tacctcagga caaaccggat gccagataaa
     3901 atcactcttt tcacgcccca taaagcctgt gatatcctga ccctttccgt tgtatctgat
     3961 tttagatcca aactgacagg ctgaaatacc gatgttgatt ttctctgata aaaagttatt
     4021 cattgattta ttggaaatcc ttgttatctt acaattttca gtttatagcc attttttaaa
     4081 acttacaagt ccataaaagc aaatttgcag aatatccgag accctaaata agcaagctga
     4141 cctaaactca ctcacactcc atttctccat catatttgca ttatttttga aattcgccaa
     4201 acaaatcttt tgaattcttt ttttaataaa tctgatattt caacgatatc tttatggctg
     4261 tgcacaagaa agaattcttt cgcaagaagt ctgattttct tatcgtctgt caagtcgact
     4321 acaacatcag ggtcgccatt gaatatttgt gcgtatccat tatagttttc tttaattatt
     4381 tctacggcag gtctctttgt gtctttgagg ggaaatccgg ttttaagatg tacatttttt
     4441 attactagcg caataagctt cattacagtc cggtttctat cgtcttcgat atccttatgc
     4501 atcttttcaa aagccttaat gatatttttg tttttatctt taatgcggtc aataagtggc
     4561 aattgaattt cgactggaag cattttgaat tcaggttctt gtaaaggata aacacattta
     4621 agataacggc gtgagagcat aacacgcccg gcaaaagtat catttattgt atcagcattt
     4681 acaaaatcgg aaacagaatc aataggcata tacagaagac tttttagacc cttaaattgg
     4741 gcaggcaatt ccatattttt aaatattgcc aaaattgatt tttcttcaca ctcatgtttg
     4801 aagaaaaact ctatcattag atgctcaaaa attgccttat cgtttcctga aatagatgga
     4861 ttttcgtcca tagttaaaac aaattctaga aggttataaa acgattctcc cttagtctgt
     4921 ctcatgacta taccctaatt tttgctgtta catccgcttc aagtgtgtca aactggtctc
     4981 ggcaaaagta gtgataagac accccggcaa tcatcgcggc attatccgta caaagatcaa
     5041 ttggaggtat tatgattttt tcaccatcac gacattccga caaaaaaagt tctcttaatc
     5101 tgctattggc cgctacgcca ccggcaataa ctatgttttt taaaccataa tctcgtgctg
     5161 cggcaaaagt tcttcgtatt aaaatttcga tagcgctctc ctgaaacgaa taggcaactt
     5221 cattaatatc atattctgga ttatttttca cataattaat cactgctgtt tttaaaccag
     5281 agtacgaaaa tctgtaatcc tcatttttcg ccgccaaaag tttcggaaac aatttctcct
     5341 ttttcacggc ttttcgagcc agcttgtcaa ttaacggccc tccgggataa ccaagctcca
     5401 taaattttga caccttatca aaggcctctc ctatagcatc gtccatggtt cgccctaata
     5461 gcatcatctc tccaattccc ttcatcaggt aaagagcggt attgccgcct gaaacaagaa
     5521 ggccaatgta agggtagcta atggtttcac tgtttaaaaa tggagcataa agatgagctt
     5581 caagatgatt agttgcaata agaggaattt tttttatata actgatcgtt ttggcagcct
     5641 gaagcccgat cataagagag cctatcagcc ctggtctgtt tgtcgcggct acataatcaa
     5701 tatcgtcaaa ggtaagccca ctctctttca aggcagaatc aataagtgga ttaatcatct
     5761 ccaggtgagc tctggatgca atttctggaa caaccccata atactccgag tgaagatcta
     5821 tctggcttgc aatacgattc gataaaacac gcgagccatt ttcaacaatg gcggccgcgg
     5881 tttcatcaca agagctctca agtccaagtc caattatgtt tttcatttaa gatacttgag
     5941 tgaatctttt aactgattat caaattcaag atcgtaaaga gcatccggtt tatcacggtt
     6001 aagctcaaac ttgtaataaa aggccgccac ctttttagac aacggaacat tttttgattt
     6061 cagaaaagaa actaataact caatgttttc ttcattatat ttttcatact tttgagcaaa
     6121 tttttctatt aatttgtcat taaaaacacg cccaaggttc tttttatcat tttcaggtat
     6181 tatatcctgt ttaaccaaat aatcaggctt aataccagct ttatgaatca ttttacccga
     6241 tggagtgtaa tatttgcgaa tagttaaggc aaaacctgtc ttgccttttt caatatctat
     6301 cacacgttga acaagggctt taccgaacgt ggtttctcca gccagtatcg cacgcttgtt
     6361 atcctgaaga gcaccactta gtacttcaga cgccgaagcg cttccctcgt taaccagaat
     6421 cagaatatcg cctttaaaca gcacatcgtg ctcggcctta tactcttcag aaacaccaga
     6481 tccctcttta cctctggtag agactataac agaatttttt ggtaaaaaat agtccgctat
     6541 atgaatagac gtctccagat taccgcccgg gttattacgt aaatccagaa taagtgattt
     6601 tacccctttt ttctcgagat ccagaagagc ttttcttaca tctagatgtg tatcactata
     6661 aaaatt
//
LOCUS       DAOTGZ010000155         6615 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_3064
            NODE_13770_length_6615_cov_4.135366, whole genome shotgun sequence.
ACCESSION   DAOTGZ010000155 DAOTGZ010000000
VERSION     DAOTGZ010000155.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868088
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 6615)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6615)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:22:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,785
            CDSs (total)                      :: 2,751
            Genes (coding)                    :: 2,732
            CDSs (with protein)               :: 2,732
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 12 of 19
            Pseudo Genes (internal stop)      :: 5 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6615
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13770_length_6615_cov_4.135366"
                     /isolate="AMR_MDS_3064"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..372
                     /locus_tag="P5123_09900"
     CDS             <1..372
                     /locus_tag="P5123_09900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013739382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0033179 - proton-transporting V-type
                     ATPase, V0 domain [Evidence IEA];
                     GO_function: GO:0015078 - proton transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATPase 116kDa subunit family protein"
                     /protein_id="HRX16618.1"
                     /translation="PTEFFIKSKKPFKIGIIADWLMDWIVELLELFSGYLSNTLSFLR
                     IAGLGIAHVSLMNAFFQIAASSDSALVSLFVIIIGNILVIALEGLSAGIQSLRLNYYE
                     FFSKYFRGSGKVYEPISLRQK"
     gene            494..865
                     /locus_tag="P5123_09905"
     CDS             494..865
                     /locus_tag="P5123_09905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013255668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP synthase subunit C"
                     /protein_id="HRX16619.1"
                     /translation="MNSNQKVFRKRIALSLLITLFTMLITLFIFVSPAIAAEEAESLS
                     SIANEHQAEVVKWEVIAAAIAFGLGALGAGMAISRVGSAAMGAMSEKPEVGSQALIFV
                     AMAEGIAVFGFIAALMILGKF"
     gene            862..1167
                     /locus_tag="P5123_09910"
     CDS             862..1167
                     /locus_tag="P5123_09910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013255667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit F"
                     /protein_id="HRX16620.1"
                     /translation="MKYFIIGDEDTVLGFGMVGVHGRVATNEPEAQAAFEEALSLSET
                     AIIIITEQASALISGLVNSYTFSHDFPLIVEIPGMDGRDLKKPRLREMVNEAIGINL"
     gene            1174..1809
                     /locus_tag="P5123_09915"
     CDS             1174..1809
                     /locus_tag="P5123_09915"
                     /inference="COORDINATES: protein motif:HMM:NF014093.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0033178 - proton-transporting two-sector
                     ATPase complex, catalytic domain [Evidence IEA];
                     GO_function: GO:0046961 - proton-transporting ATPase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit E family protein"
                     /protein_id="HRX16621.1"
                     /translation="MEQQGQQAVISGIFEEAQKKADEIIRTANVQIDEHQKSWSIQKE
                     NILRDAEIEATRRENEKRNSIISQITVEKKRKALREREALINKLISDTRAHMADKIDK
                     PEYKNILLNWIVEAAIGLNLPRAIIKTSAAEKKIINRDFLDLVNKRASEIRGSETVVS
                     LDESDHLSCQGVVLSAEDGSVAFSNTVNDRIIRNQSDIRQAINKIIDSSDN"
     gene            1866..3620
                     /locus_tag="P5123_09920"
     CDS             1866..3620
                     /locus_tag="P5123_09920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020614151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit A"
                     /protein_id="HRX16622.1"
                     /translation="MEERSIGLVKRVNGPVIEADGITDGMMMELVRVSEARLIGEIIK
                     LSGNNAIIQVYEDTTGVAPGDIIYGSGLPLSVELGPGLIGTIYDGIQRPLEKLAEISG
                     SHIKRGIQSPSIDREKLWNYTPTINDGDFISPGTVLGTVQESDRILHKILCPPDLSGT
                     VNAVLKPGSYTVDTVIAKINDGSTVKDLTMIHRWPIRKPRPVASRIPLRIPLITGQRV
                     IDTLFPLAKGGTVAIPGGFGTGKTMTQHAIAKWCDADIIVYIGCGERGNEMTDVLNEF
                     PKLVDPRTGSSLMERTLLIANTSNMPVSAREASIYTGVTLAEYYRDQGYHVAVMADST
                     SRWAEALRELSGRMEEMPAEEGFPAYLPTRIAEFYERAGFMKTLNEEEGSVTLIGAVS
                     PPGGDFSEPVTQHTKRFVRCFWGLDRHLANARHYPAISWLESYSEYLDEVSPWWAERA
                     GDNWFRNRSRIMELLQREIKLQQVVKLVGPDALPDSQRFVIEICTLFKNAFLQQSAFD
                     KNDCYSTVEKQLRMMDIILKYAELGENSIRKGVTLVNLRRMKVLQDIVKMKLTVPNDD
                     LSLLDRIEARLERSIEKL"
     gene            3628..5043
                     /locus_tag="P5123_09925"
     CDS             3628..5043
                     /locus_tag="P5123_09925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020614150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit B"
                     /protein_id="HRX16623.1"
                     /translation="MIKTDKLDKIHKDLLKGREYIGVHQIDGPIVIIRNTHQVGYREL
                     VECIDQNRQVRLGMVLDTSKTAVAVQVFEGTSGLTLPDTRMRFKGEPLMIPVDVSMLG
                     RVFNGLGKPIDGGPPPVSTIEKDVNGLPVNPTAREYPRDFIQTGISVIDAMNTLIRGQ
                     KLPIFSGNGLPHNQLAAQIARQATLRGEKTDFAVVFAAMGIKHDIARYFIDSFETSGV
                     LDKVALFLSLADDPSIERIITPRTALTLAEHLAYDCGKHVLVIMTDMSNYCESLREIS
                     TIRGEIPSRKGYPGYLYSDLAELYERSGMIKGCSGSITQLPILTMPNDDISHPIPDLT
                     GYITEGQIVFDRDMNSRGIYPPVAGLPSLSRLMKDGIGEDMTRIDHPHLASQLFASYS
                     YVKDVRNLASVIGDEELTALDKQYMEFGDEFERRFVNQRNDENRDIEESLDIAWQCLS
                     ILPPEELTRITEDELDEHYGR"
     gene            5033..5656
                     /locus_tag="P5123_09930"
     CDS             5033..5656
                     /locus_tag="P5123_09930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013739376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit D"
                     /protein_id="HRX16624.1"
                     /translation="MADNRPAPTRTNLIRLKQQLEFASQGYELLDQKRNILIMELLNL
                     IDLAVDFEEKSDFTLQQAHKSLRLAVIENGKLKAAALSSAINIESSITLTSRRIMGVS
                     LPVVRTTFKEHGPYYAPEGTSSWIDNAINDFKQILSIMGELAELKISIMRLAREVRKT
                     IRKVNALEKIAIPDLTQSIRYIQNRLEEDERDRYILMKRVKARLSNN"
     gene            5672..6160
                     /locus_tag="P5123_09935"
     CDS             5672..6160
                     /locus_tag="P5123_09935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020614148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0032559 - adenyl ribonucleotide binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HRX16625.1"
                     /translation="MDNPVERVLVYLDGSIESVTAARYAICLCKKLNAKLYGTFIINT
                     HALNDLVKSRIFIESEQIEYKNDLEADADKYLRNFETEAVSKGLAPECIKESGLVNVK
                     IKELVRSYEIDLFVIGELSRIRSRRDEFHNESERAMRSVPCSVLIVKDEDRVDDIYEN
                     LN"
     gene            6173..>6615
                     /locus_tag="P5123_09940"
     CDS             6173..>6615
                     /locus_tag="P5123_09940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020614147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HRX16626.1"
                     /translation="MTLLEMINEDVIKVPLESSTKHDVFRELIEILHKAGKVKNTESV
                     FDAVINRDNQMSTALENGIALPHAKTEEVNQLVLAIGVSPKGIDCDSHDGNLSHLFFL
                     LLAPPDQSGPHIEALSEISRATKSNAFRRVLLSAQNAAEIVEFFC"
BASE COUNT         2076 a         1286 c         1421 g         1832 t
ORIGIN      
        1 cctacagaat tttttataaa aagcaaaaaa ccgtttaaaa tcggtataat cgcagattgg
       61 cttatggact ggattgtcga actgctagag cttttttcgg gatatctttc taatacactc
      121 tcttttttac gaattgctgg tcttggaatt gctcatgtaa gtttaatgaa cgctttcttt
      181 caaattgcgg cttcatctga ttcagcgctt gtgtcactct tcgtgattat tattggaaat
      241 atactagtaa ttgcccttga agggctatca gccggtatac agtcactgcg cttaaattat
      301 tacgagtttt tctcaaaata ttttcgcggt tcgggtaagg tttacgaacc catttcactt
      361 cgtcagaaat aaataggagg caatagaaac ttctaaaatt actattttta gaatgtaaca
      421 gctaataatt gttattttgg ttattagagg ttgcctaatg ttttaagctt attgataata
      481 caggaaggta aaaatgaact caaatcaaaa agtctttaga aaaagaatag ctcttagtct
      541 tctcattact ctatttacaa tgctaataac gctatttatt tttgtttctc cggcaatagc
      601 cgcggaagaa gccgaatctc taagctctat tgcaaatgag catcaagcag aagtagtaaa
      661 atgggaagta attgcagcag caattgcttt cggtcttggc gctttaggtg ccggtatggc
      721 tatctcacgt gtaggctcgg cagcgatggg cgctatgagc gagaaacctg aagtcgggag
      781 tcaagctcta atatttgtgg caatggctga aggaattgct gtttttggtt ttatcgcagc
      841 gttgatgatt ctgggtaaat tttgaaatat ttcattatcg gtgacgaaga cactgttctc
      901 ggatttggca tggtgggtgt tcacggacgt gtagccacta atgaaccaga ggcccaagcg
      961 gcttttgaag aagcattgtc cttatccgaa acagctatca taattattac agaacaggcg
     1021 tccgccttaa tcagcggact agttaatagt tacacatttt cccacgattt tccactaata
     1081 gtggaaattc caggtatgga cggtcgtgat ctgaaaaaac cccgattacg ggaaatggtg
     1141 aatgaggcta taggtatcaa tttgtaggta aatatggaac aacagggtca acaggcggta
     1201 ataagcggta tatttgaaga agctcaaaaa aaagctgatg aaataatcag aaccgcgaat
     1261 gttcaaattg atgaacacca aaagagctgg tcaatacaga aagagaacat tcttcgtgat
     1321 gccgaaatag aggcaacccg aagagaaaat gaaaaacgga attctataat ttctcaaata
     1381 actgtggaaa aaaaacgcaa agcccttcgc gaacgtgaag cgcttatcaa caaactcatt
     1441 tctgacaccc gagcacatat ggcagacaaa atagataaac ctgaatacaa gaatattctg
     1501 ttaaactgga ttgttgaggc ggccattggc ttgaatcttc ctcgtgctat cattaaaaca
     1561 tctgcagccg aaaaaaaaat tattaacagg gattttctag atttagtaaa taaacgggct
     1621 tcagaaatca gaggcagtga aacagttgtt tcattggatg aatctgatca cctaagttgt
     1681 cagggcgtag tactaagcgc cgaagatgga tctgtagcgt ttagtaatac tgttaacgac
     1741 agaattattc gaaatcagtc agatatacga caggcaatta acaaaattat tgatagttct
     1801 gataactaac aaaatattgt gatgcattat tgtttcgata aggcatcttg caaaatgagg
     1861 tttaaatgga agagagaagc atcggactgg taaaacgggt taacggcccg gttattgaag
     1921 ccgatggaat aaccgacggc atgatgatgg aactcgtacg tgtttctgaa gcaagattaa
     1981 ttggagagat aattaagcta tccggtaaca atgccattat tcaagtatat gaggatacaa
     2041 caggcgttgc accgggcgat attatttacg gttccgggct gccgctttct gttgaattag
     2101 gccccggtct tatagggaca atttatgatg gaatacagcg cccactggaa aaactggctg
     2161 aaatatcggg ttcacatatt aaaagaggta ttcagtcacc atcaatagat cgtgaaaaac
     2221 tctggaacta cactccaaca ataaatgacg gagatttcat atctcccgga acagtactcg
     2281 gaacggtaca ggaaagcgac cgcatattac ataaaatact ctgtccacca gatctgtctg
     2341 gcacagttaa cgctgtactt aaacccggaa gctatactgt ggatacggtt attgcaaaaa
     2401 ttaatgatgg ttcaactgtt aaagatctca ccatgattca tcgctggccg attagaaagc
     2461 cccggcctgt agcaagccgt attcctttac gcattcccct tatcacaggg cagagagtca
     2521 ttgacacact atttcctctt gcaaaaggtg gtacagttgc cattccaggt gggtttggaa
     2581 cggggaaaac catgacccag cacgccattg ccaaatggtg tgacgccgac attatagtct
     2641 atattggttg tggcgagcgc ggtaatgaga tgaccgatgt acttaacgag tttcctaaac
     2701 ttgttgatcc tcgtacagga agcagtctaa tggaacgaac tttactgatc gccaacacgt
     2761 ccaacatgcc tgtatcggct cgagaagcaa gtatctatac cggagtaaca cttgcggaat
     2821 actatcgcga ccaaggatac catgtagcag taatggccga ttctacttca cgttgggcag
     2881 aggccctcag agaactctca ggtcgtatgg aagagatgcc ggccgaagag ggtttccctg
     2941 cgtatttacc taccagaata gcagaattct atgaacgagc cggttttatg aaaaccctta
     3001 atgaagaaga gggctcagta actcttatcg gagcagtttc tcctcctgga ggcgatttct
     3061 cggaacccgt aactcagcac acaaaaagat ttgtccgttg cttctgggga ctagatagac
     3121 atctggcaaa cgctcgccac tacccagcca tatcctggtt ggaaagctac tcagaatatc
     3181 ttgatgaagt ctcgccatgg tgggcagaaa gagccggtga caactggttt cgcaaccgtt
     3241 cacgcatcat ggaactttta caacgtgaaa taaaactaca gcaggtagtt aaacttgttg
     3301 gtcctgacgc actacccgac agccagagat ttgtgataga aatatgcaca ctttttaaaa
     3361 acgcatttct gcaacagagc gcttttgaca aaaacgactg ctattctact gtcgaaaagc
     3421 aattacgtat gatggatata atcctcaaat atgccgagct tggtgaaaac tccattcgca
     3481 agggagtaac tttagttaat ctaagacgta tgaaagtgct tcaagatatt gtaaaaatga
     3541 aattgactgt tcctaacgac gatctttcac ttcttgaccg tattgaagca cgtcttgaaa
     3601 gatcaataga aaaactgtag gtaaaaaatg ataaaaactg ataaactgga taaaattcat
     3661 aaagatcttc ttaagggccg tgaatatatc ggtgttcatc agattgatgg tccgattgta
     3721 attattcgca atacccatca ggttggttac cgcgaactgg tcgagtgtat cgatcaaaat
     3781 agacaggtta gattaggaat ggtactggat acatcaaaaa ctgctgttgc cgtccaagtt
     3841 ttcgagggca ccagcggcct aaccttaccc gacacaagga tgcgttttaa gggtgaacct
     3901 cttatgattc ctgttgacgt atcaatgctt ggacgtgtct ttaatgggct ggggaaaccc
     3961 attgacggtg gtcctccgcc cgtatccaca attgagaaag atgtaaatgg gctacccgtt
     4021 aatcctacag ctcgtgaata tccgcgtgac tttattcaga ctggaatttc tgttattgat
     4081 gcaatgaata ccctgatacg tggacaaaaa cttccaatat tttctggtaa cggacttccc
     4141 cataatcagc ttgcggctca gattgcccgt caagcaacat tacgcggtga aaaaactgat
     4201 tttgctgttg tttttgccgc tatgggcatc aaacatgata ttgctcgcta ttttatagac
     4261 tcctttgaaa cttcaggagt actcgacaag gtcgcgcttt ttttatcttt agccgatgat
     4321 ccctccatag aacggatcat tacgcctcgc acagctctta ccttggctga acatcttgct
     4381 tatgactgcg gcaaacacgt tttggtaata atgaccgata tgtcaaacta ttgtgaatcg
     4441 ttaagagaga tttctacaat acgaggggaa ataccttcca gaaaaggata tccaggctat
     4501 ctctactctg atctcgcaga actctatgag agatcaggca tgataaaagg gtgttccgga
     4561 tcaataactc agcttccgat acttactatg cctaatgatg atatctctca ccctatacct
     4621 gatctaacag gttatataac agagggtcag attgtttttg acagggacat gaatagccgt
     4681 ggtatttacc ctccagtcgc tggattaccc tcactttcga gacttatgaa agacggtatc
     4741 ggtgaagata tgacaagaat cgatcaccct catttggcaa gccaactctt tgcatcttac
     4801 agttatgtaa aggacgtacg taatttagca tcggtaatag gtgatgaaga gctaacagct
     4861 ttagacaaac aatatatgga attcggcgat gaatttgagc ggcgctttgt aaaccagcgc
     4921 aatgatgaaa atcgcgacat cgaagagagc ctcgatatag catggcaatg tttaagtatt
     4981 cttcctccgg aagaacttac acgaataact gaagatgaac tggatgaaca ctatggcaga
     5041 taatcgccca gctcccacac gaacaaatct gattcggctc aaacagcaac ttgaatttgc
     5101 ttcacaggga tacgagcttc tggatcaaaa acgcaatata cttattatgg aacttcttaa
     5161 cttaatagat cttgctgttg attttgaaga aaaatcagat tttacacttc aacaagctca
     5221 caaatcactt cgacttgctg ttattgaaaa cggcaaacta aaagctgctg ctctatcctc
     5281 tgcaattaat attgagtcat caattacact gacttccagg cgtattatgg gagtatcgct
     5341 tccggttgta cgaacaactt ttaaagagca tggcccctat tacgctcctg agggtacaag
     5401 ttcatggatt gataacgcaa ttaatgactt taagcagata ttgagtataa tgggtgaact
     5461 ggcggaactt aaaatatcga ttatgaggct ggctcgtgaa gtacgtaaaa ccatacgcaa
     5521 ggtaaatgct ttagaaaaaa ttgccatacc agatctgaca cagtccatca gatacataca
     5581 aaacagactt gaagaagatg aaagagatcg ttatattctt atgaaaagag ttaaagcccg
     5641 tttaagtaac aattaatgaa tggagataaa tatggataat cctgtagaga gggttcttgt
     5701 ttacctagac ggctctatcg agtcggtcac cgcggcacga tatgccatct gtttatgcaa
     5761 aaaattgaac gcaaaactat acggtacatt tatcattaat actcatgcac ttaatgatct
     5821 tgttaaaagc cgtattttta ttgaatctga acagattgaa tacaaaaacg atcttgaagc
     5881 tgatgcagat aaatatttaa gaaattttga aactgaagct gtttcaaaag gtctggctcc
     5941 cgaatgcata aaggagagcg gattggtcaa tgtaaaaatt aaagagcttg tcagatcata
     6001 cgaaattgat ctatttgtaa ttggtgaact atccaggatt agaagtcgta gagatgaatt
     6061 tcacaatgaa agtgagcgag ccatgcgaag tgtgccctgt tcagttttaa ttgtaaaaga
     6121 tgaagatcga gtcgatgata tctatgagaa tctcaactaa ggggtaacta acatgacatt
     6181 attggaaatg ataaacgaag atgtaataaa agtaccactt gaaagtagca cgaaacatga
     6241 tgtctttcgg gaactgattg agatacttca caaggccggt aaggtaaaga acaccgaatc
     6301 ggtatttgat gctgtaatca accgcgataa tcagatgagc actgcacttg aaaatggcat
     6361 tgctttacct catgcgaaaa cggaagaggt taatcaacta gtactggcaa ttggagtttc
     6421 accaaaagga atagactgtg attcacacga tggcaactta tctcatcttt tctttctatt
     6481 gttggctcca cctgatcaat cgggtcctca tattgaagca ctatcggaga tatcgcgcgc
     6541 aactaaaagc aatgcctttc gacgggtgct tttgtcagct caaaacgcgg ccgaaattgt
     6601 agaatttttc tgtga
//
LOCUS       DAOTGZ010000156         6610 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_3064
            NODE_13782_length_6610_cov_4.847902, whole genome shotgun sequence.
ACCESSION   DAOTGZ010000156 DAOTGZ010000000
VERSION     DAOTGZ010000156.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868088
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 6610)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6610)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:22:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,785
            CDSs (total)                      :: 2,751
            Genes (coding)                    :: 2,732
            CDSs (with protein)               :: 2,732
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 12 of 19
            Pseudo Genes (internal stop)      :: 5 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6610
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13782_length_6610_cov_4.847902"
                     /isolate="AMR_MDS_3064"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(111..1583)
                     /locus_tag="P5123_09945"
     CDS             complement(111..1583)
                     /locus_tag="P5123_09945"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRX16627.1"
                     /translation="MKRALCIISIFLFINIEAAGLKKHIDTFGYDFLNTTITDIEKSE
                     LEKISFENSSPYYPDKIIRLYLIASQINKEPDFTRELNAFESVIKKIEPQTKGLSSYN
                     KAEKILHILHDIYFKTELTGSGAGYNIGIEDTLRNRRFNCYKSALIYNAVLEYFGYES
                     YLVIVPEHIFSVVLINGKQIEVETTNRFGFDPYDRGAPGYKREFDKPGVIFDKKNYSS
                     KTPVDNISIISIIYSNRLLLYTGQKRYTGYSVKPDFERAAALGVLGLYMHGKSTNLAN
                     NIIYPFYRISSGNMKKDPASISKYVKQFSEFLAHSRFAPYRPMYNKNISIAVLESQPA
                     LRESYLSSANPISADTVTDAYIQSLKSVESNSQSAEITETAWNNITIDYNHDLAKQIN
                     TDSIEGIRDYALQQKRLFETLSAYKHSSKYKNMAAYNTTVILNNYILKIYGTKNYQKA
                     LSEIDRGIDLLEKQLSFYDKKVMDSVKKNRAIIVEAIGRQ"
     gene            complement(1630..4851)
                     /locus_tag="P5123_09950"
     CDS             complement(1630..4851)
                     /locus_tag="P5123_09950"
                     /inference="COORDINATES: protein motif:HMM:NF013069.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HRX16628.1"
                     /translation="MEKFFVKFIRLLVNRPLLVNLLLIFIIITGAYSLYTMQRLGLPR
                     VELYMTHITTIYPGASPEDVELNVTKKIETALESVSDIKKYTSVSMENMSFVTVEINA
                     STDNLKEVQDDIERAVNRINDFPEEITDKPVFFEEKSDNWSIMSIGFSSNSKNGKEYV
                     QLAKDLKEELLEVPQVSGIDIEGDYENEIVIKLKKSKLNEYIISIEEVINSIKSNKIR
                     LSAGSLESFTTETGIVTLSEFETVEEIANIIVRINDAGNVIRLGDIATITKQIEEGDV
                     IRKVNGHEGLWLEVIKQGNADILDTVNGINRVIERFKESRHIDETVDIIVLRDESIET
                     RERLFILYTNAGIGLALVLILLFFFFSKRIAFWTSLGIPVAFATAFIVASLAGVSINR
                     VSLLGLVVVLGMLVDDSIVVAENIHRHRLMGYNGKESAARGTAQVLIPVIGTVLTTVI
                     AFVPIYFVPGLAFDFSKEIPTFVIAMLVGSLIESVFVLPVHLAHEKDDKETIEKDPFG
                     MKLLHFMENAYERFLRRMLQHRYLSFGATLLIFAAALLLSLALTKFVMFPIDQATILT
                     IYGETEPASSLSNTDSIISPVNDIIETLPDNVIKSYQTITGKKGRYSSRNLPNYFTFE
                     LTLTTSTERDMTALQVKDYIFSEIDKNGITKIETIDYEIDGGGPPAGKPVSIDVIGND
                     NEKRKKLISQIMKDLENIGLTEIDTDFREGKVEIRLAPDYELIARGDLSVGQVANVIR
                     TAFDGTIVTYMETADEQIPFRVVLDKKDIDIEHPLRGLYTINNRGILIPVENLLRSQK
                     GSTPQTVYRYNGKRKNTVTANIPDGMTSIEIYDKLGSLYTNFTKDNPGFRINLGGEAE
                     ESTNTRNKMIFATVLAIIGIYFVLVLQFNSLIQPAIVISAIPFGLIGILLAFGLQRID
                     LSMLALVGIMGYGGVVVNNSLILVEFINNERKSSDNSNFIENVVNGSKMRLTPIFLTT
                     LTTVAGLMPTAYGWFGGMDSFISPMIMAMAWGLIIGTPAVLFVIPIQYAIIEDVVTFA
                     KRITGIEPKESDFNNRPDSNLETKNNSIATRKNNLVYKKGGNR"
     gene            complement(4905..5501)
                     /locus_tag="P5123_09955"
     CDS             complement(4905..5501)
                     /locus_tag="P5123_09955"
                     /inference="COORDINATES: protein motif:HMM:NF012655.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HRX16629.1"
                     /translation="METKEKILNSAAKIFAEKSYDGSRVDQIAEHAGIPKSLIYYHFK
                     SKESILNTLIEAFISDLVRIQNNIKEIYEHEKTIAPNEQIIEKVVPFFQEKSDIMRII
                     MAESLKVCPSPPIFRVLKTVIDSKRSAACRFNNIDYDNPKTVLREFINYIIPVFTFSC
                     LYNSFSDIYGFDRNLIIKHFIDIMDQNSCENISIFENK"
     gene            complement(5884..6610)
                     /locus_tag="P5123_09960"
                     /pseudo
     CDS             complement(5884..6610)
                     /locus_tag="P5123_09960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000488887.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=2
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein"
BASE COUNT         1824 a         1233 c         1265 g         2288 t
ORIGIN      
        1 cttctattaa ataatacacc ggcgcgtctt tacgatgctg ttctcgctat attcttgtat
       61 gcagaaaaag atctgttttt catttttcat ttttcattat tcactgcctc tcactgcctt
      121 ccaatggctt ctacaataat tgcgcggttt tttttgacgg agtccatgac ctttttgtca
      181 taaaaggaga gttgcttttc gagcaggtct atgccgcggt caatttcgga taacgctttt
      241 tgatagtttt ttgttccata aattttcaga atatagttat taagaattac cgtggtatta
      301 taggcagcca tgtttttgta tttgctgctg tgcttgtatg cggatagtgt ttcaaagagt
      361 cttttctgct gcagtgcgta gtctcgtatg ccctctatag agtcggtatt aatctgcttg
      421 gccagatcat gattataatc aatggtgatg ttgttccagg ctgtttcggt tatttcggca
      481 ctttgtgaat tactctcaac agattttagc gattgaatat acgcatctgt gactgtatcg
      541 gcactaatcg ggtttgcgga cgacaaatag ctttcacgca gagccggttg tgattccaga
      601 actgcaatgc tgatgttttt attgtacatg gggcggtagg gggcaaaacg cgaatgcgcc
      661 agaaattcgc taaactgttt tacgtattta ctaattgatg caggatcttt tttcatgttg
      721 ccggagctta ttctgtaaaa tggatagatt atattgttgg caaggttggt gcttttaccg
      781 tgcatataca gacccagaac tcccagcgcg gctgcacgtt caaaatcagg tttaactgaa
      841 tatcccgtat aacgcttttg accggtgtaa agaagaagtc ggttgctgta aattatactt
      901 atgattgaaa tgttatcaac cggtgttttg ctgctgtagt tttttttgtc aaaaataacg
      961 ccgggtttat caaattctct tttatatccg ggggcgccgc gatcataggg atcaaatccg
     1021 aaacggtttg tcgtttcgac ttctatttgc tttccattga tcagtacaac agagaaaata
     1081 tgttccggaa caatcaccag gtaactttca taaccgaaat attcaagtac tgcgttgtaa
     1141 atcagtgctg acttgtaaca gttaaagcgt ctattacgta atgtgtcttc gataccaata
     1201 ttgtaaccgg caccgcttcc ggttaattcg gttttaaaat agatatcgtg caaaatatgt
     1261 aaaatttttt ctgctttatt ataagaagag agaccttttg tttgcggttc aattttcttt
     1321 ataacagatt caaaggcatt aagttctcga gtgaaatcag gttctttgtt aatctgtgag
     1381 gcaataaggt aaagccttat aattttatcg ggatagtagg ggctactatt ttcaaaactt
     1441 attttctcta attcgctttt ttcaatatct gttattgttg tgttcagaaa atcataacca
     1501 aaagtatcga tatgtttttt tagtccggca gcctctatgt ttataaaaag aaaaattgat
     1561 attatacaaa gtgctcgttt cattatttat tccgtaataa ggttttataa atatagaggc
     1621 ttgatctgct tatctgttac cacctttttt gtaaactaaa ttgttttttc ttgttgctat
     1681 ggagttattt ttggtttcaa ggttagagtc tggtctatta ttaaaatcgc tctcttttgg
     1741 ttcgataccg gttattcgtt ttgcgaaggt aacgacatct tcaattattg cgtattgtat
     1801 gggaataaca aagagtaccg cgggggtacc gattattaac ccccaagcca tagccataat
     1861 cattggtgag ataaacgaat ccatacctcc aaaccagccg tatgctgttg gcattaaccc
     1921 cgcgacagtt gttagggttg tcaaaaaaat aggcgtaagt cgcatcttag atccgtttac
     1981 aacattttcg ataaagttag agttatcact gcttttacgt tcattgttaa taaactctac
     2041 aagaataagc gaattattta caacaacacc gccatagccc attattccca caagagccag
     2101 cattgataga tcaattcgct gtaatccaaa tgctagaagt atgccaatta aaccaaatgg
     2161 tatagccgaa attactattg ctggttgaat caatgaattg aactgaagaa ctagaacgaa
     2221 gtatattcct attatcgcaa gaaccgttgc gaagatcatc ttgtttcgag tgtttgtgct
     2281 ctcttcggct tcaccaccca gattaattct aaaaccgggg ttatcttttg tgaaatttgt
     2341 gtataaggaa cccagtttgt catatatttc gatagaggtc atgccatcag gaatatttgc
     2401 cgttactgtg tttttacgtt ttccgttata tcgataaact gtttgaggag tcgatccctt
     2461 ttgcgaacgt aggaggtttt cgactggtat cagtataccc cgattattga ttgtatagag
     2521 gcctcttaag ggatgttcga tatcaatatc ttttttatca agaactactc taaacggaat
     2581 ttgttcgtcg gcagtttcca tatatgttac tattgttccg tcaaaagctg tacgaattac
     2641 atttgcgact tgaccaacag agagatctcc tcttgctatc agttcgtagt caggagcaag
     2701 tctaatttct actttacctt cacgaaagtc ggtatcaatt tcggtcaggc caatattctc
     2761 aagatctttc attatctgtg aaatcagctt tttcctcttt tcgttatcgt tgccaataac
     2821 atctattgat acaggttttc ctgcgggtgg tcctccaccg tctatttcgt aatcaatggt
     2881 ttcaattttc gttatgccat ttttatcaat ttcagaaaaa atgtaatcct taacctgtaa
     2941 cgctgtcata tctcgttcgg tagaagtggt aagtgtcaac tcgaaggtaa agtagtttgg
     3001 caggtttctt gatgagtacc ttcctttttt gccggtaatt gtctggtaag acttaataac
     3061 attatccgga agtgtttcga tgatatcgtt tactggtgag attattgaat cagtattgga
     3121 aagagagctt gctggttctg tctcgccata tatcgttaga attgttgcct ggtctattgg
     3181 aaacattaca aatttcgtaa gtgccaagga aagtaaaagt gctgctgcaa agataagcag
     3241 agtggcacca aaagataaat aacggtgttg tagcattcta cgtaaaaagc gttcataggc
     3301 attttccata aaatgaagaa gtttcattcc aaaaggatct ttttcaatcg tctctttatc
     3361 atctttttca tgtgctaaat gcactggtaa aacaaaaaca gattctataa gacttcccac
     3421 aagcattgcg attacaaaag taggaatttc tttagaaaaa tcaaatgcca gtcctggaac
     3481 aaaatatatt ggtacaaaag caataactgt tgttaaaaca gtaccgataa ccggtattaa
     3541 aacctgagca gtaccacggg ctgccgactc tttgccgttg taccccatta gacgatgtct
     3601 gtgaatattt tccgcgacta caatggagtc atcaacaagc ataccaagta cgactacaag
     3661 acccagaaga gaaacccggt taattgaaac tccggcaaga gacgcgacaa taaatgctgt
     3721 cgcgaaggct acaggtattc caagagatgt ccagaatgct attcgcttgc taaagaagaa
     3781 aaatagcaga ataagaacaa gcgcaagtcc aatcccggca tttgtgtata atataaaaag
     3841 tcgttcgcgt gtttcgatag actcatcacg caagacgatt atatctactg tttcatcaat
     3901 atgtcggctt tccttaaacc gttcaataac tcggttaatt ccattgacgg tatcaagaat
     3961 atcagcatta ccctgtttta tgacctctaa ccaaagccct tcgtggccat taacttttcg
     4021 tataacgtca ccttcttcga tctgttttgt tattgtcgca atgtctccaa gtcgtattac
     4081 attgccggca tcgtttattc gaacaattat atttgctatt tcttcgacag tttcaaattc
     4141 agataatgtt acgattccgg tctcggtggt aaaagattca agagatccgg cagaaagccg
     4201 tatcttgttt gattttatag agttaataac ttcttcaatg gatattatgt attcgttgag
     4261 tttagatttt ttgagtttaa ttactatttc attttcgtaa tcgccttcga tatctattcc
     4321 agagacttga ggtacctcaa gcaactcttc cttcagatct ttcgcgagct gaacgtactc
     4381 tttgccattt ttgctgttag aagaaaagcc tatagacata attgaccagt tatccgactt
     4441 ctcttcaaaa aagacaggtt tgtctgttat ctcctccgga aagtcattga tacgattaac
     4501 ggcacgctcg atatcatcct gaacctcttt cagattatcg gttgatgcgt taatctctac
     4561 tgtaacaaat gacatgtttt ccatcgagac agaggtatat ttcttaatgt cactgacaga
     4621 ttccagagca gtctcaattt ttttagtgac gttaagttct acatcttccg gagaggctcc
     4681 aggataaata gtagtaatgt gggtcatata gagttcaact cgtggtagtc ctaaccgttg
     4741 cattgtataa agagagtagg cgccggtaat tataataaat atcagcagaa ggttaaccag
     4801 aagcggtctg ttaaccagaa gccttatgaa ttttacgaaa aatttttcca tggttatcct
     4861 ttgtttgaag tattgttatt tttatgtgat atagaaattt aaaactattt attttcaaat
     4921 atgcttatgt tttcacagct gttttgatcc ataatatcta tgaagtgctt gataatcagg
     4981 tttctatcaa atccgtaaat atccgagaaa gaattataaa gacagctgaa agtaaatacc
     5041 ggtataatgt aatttataaa ttcacgtaaa accgttttcg gattgtcata gtcaatatta
     5101 ttaaacctgc atgcggcact tcttttagaa tcgattactg tttttagcac acgaaatata
     5161 ggaggagaag gacaaacttt cagggattct gccataataa ttctcatgat gtcagatttt
     5221 tcttgaaaga acggaactac cttctcaatt atttgttcgt ttggagcgat ggtcttttca
     5281 tgttcataaa tttcttttat attattctga attcttacaa gatccgagat aaatgcttcg
     5341 ataagagtat ttaaaatgga ctctttgctc ttaaaatgat aatagatgag agatttgggt
     5401 attccggcgt gttcagctat ctgatcgact cgggaaccgt cgtagctttt ttcagcaaaa
     5461 attttcgccg cagagtttaa aattttttct ttagtttcca taagttaaac tgaccattca
     5521 gtactaaaac tgtcaatgat ttactattag tttacttttt aaaacagata atttatcaaa
     5581 aagtgttact tttaattcac attattcggt ttatactgtg cttatagggc agagctctac
     5641 ttatcaggca taaagacggc tcatctttgt accggataag tagatatttt cagattaact
     5701 gaaaaatatt ttcgcttagt ctatttcgat tttaatcgac tttatcttct ttttttaatc
     5761 tttatcctgt ttatgcttag acttttccgg gtttttcact ggttcaacgg gcttgctgcc
     5821 tgaatcactc tttttcgtac tttctgtatg ctttaatgtc tcattctttg tatcagataa
     5881 tgttttaaag aagtttacca tactcttaag ttcagatgct tggtctttca tcattttgga
     5941 tatggctgcc agctcttctg aagctgagga tgtgttttgc gttacaatac taagctgttc
     6001 cattcctcca ttaatttggt tgatcccgat atcctgctgt tcggacgcga gtgtgatctc
     6061 ctgtatgagt gaagcagttt ttttaatatc cggtacgatc ttgtttatta aacttccggc
     6121 cttatcagag attacgacac tatcgtcggc taccgagcgg atctcctgag cagaaaactg
     6181 gctcttctct gcgagttttc gtacttctga agcgaccacg gaaaaacccc ttccgtgttc
     6241 accggcacga gcagcttcta tcgcggcgtt taaggcgaga agatttgtct ggtaggcgat
     6301 atcttcgatt atcccaattt tttcggcaat ggatttcatc gcgttaaccg tttggattac
     6361 cgccttccca ccctcttcgg cgttaattga tactccttgt gccagagtgt ccgtattttt
     6421 agagttttcg gtattcatgc ttattgtgga tccaatctct tcgagtgaag aagagatctc
     6481 ctcgacattg gcggcctgtt cgtttgttga ttcgcttaga gacatagccg ttgcgttaac
     6541 ttcgtctgcg tatgttgtta ttgtctgaga agaagcgtga atatgcgaaa ctattgtgtt
     6601 aagtgataaa
//
LOCUS       DAOTGZ010000157         6609 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_3064
            NODE_13783_length_6609_cov_4.419133, whole genome shotgun sequence.
ACCESSION   DAOTGZ010000157 DAOTGZ010000000
VERSION     DAOTGZ010000157.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868088
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 6609)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6609)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:22:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,785
            CDSs (total)                      :: 2,751
            Genes (coding)                    :: 2,732
            CDSs (with protein)               :: 2,732
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 12 of 19
            Pseudo Genes (internal stop)      :: 5 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6609
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13783_length_6609_cov_4.419133"
                     /isolate="AMR_MDS_3064"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..611
                     /locus_tag="P5123_09965"
     CDS             <1..611
                     /locus_tag="P5123_09965"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRX16630.1"
                     /translation="FIASVAFFVFNTIFETSDSTEVSTEHFRLDNHRYPLIDTASGES
                     RFTYKNENDLLSDYEKSRGYIKSGNHNDALLLLNRIMLSNVNISVKDRVVFLKDFIRR
                     VEKKSYDKIDFAKLKADPELYNGFYIIMSGKVANFKSNDESSAFTLLVNEKNDNFDAV
                     AQVIMDGTTSVENGVSVTVKGEFLKSADDGGIIIKADNVIAD"
     gene            755..1525
                     /locus_tag="P5123_09970"
     CDS             755..1525
                     /locus_tag="P5123_09970"
                     /inference="COORDINATES: protein motif:HMM:NF024118.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HRX16631.1"
                     /translation="MPSLKFLGTGTSHGVPEIACSCDICTSTNSKNRRLRSCALLQEN
                     GRNLLIDCSKDFREQALAHKINNITDILVTHAHADHVFGLDELRVYNRLHKSRINLYM
                     SSECDNSLRILFPYIYGQPLQKGGGVSLLDNHVVQPGIAFDAAGFNIIPIPVMHGKLP
                     IYGYRIGDLAYITDCSYISEESFTLAQGVKVLVLGALRYREHPTHFNLDQAVEAAQII
                     GADQTWFVHISHNLDHDKVNSELPENINLAYDGLTLRF"
     gene            1831..2682
                     /locus_tag="P5123_09975"
     CDS             1831..2682
                     /locus_tag="P5123_09975"
                     /inference="COORDINATES: protein motif:HMM:TIGR01784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rpn family recombination-promoting
                     nuclease/putative transposase"
                     /protein_id="HRX16632.1"
                     /translation="MTYLSLKSDYVFKKLFGEEPDILMDLLNSILDLPSEQTIKELTV
                     LNPEIPKELVSEKTSIFDIRALNQNGEQIIIEMQAFPQYFFVQRALFYWAKTYASQLE
                     KGDNYQQLKKVYSINFLDFNLIDTKEYHTVFDIISRDNTDIQLTEDFEMHFLEIKKFP
                     NTTAFTDKLDIWMYIINNTEMIEENVMKTIVDKQPLMKQVFDRLDKMSMNKTLLTEYE
                     IRKRAVMDEKARTQYSYNKGIEDGIEQGIEKEKLQTASKLLAKGFTVDVISDLTGLST
                     DKIEDLR"
     gene            2889..6143
                     /locus_tag="P5123_09980"
     CDS             2889..6143
                     /locus_tag="P5123_09980"
                     /inference="COORDINATES: protein motif:HMM:NF019315.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HRX16633.1"
                     /translation="MWNLYLLRVFKNHFFLSFFMPLILLLSSSNCYSAQDPITLDLTI
                     QDLSSRSFQIDSDWEFYPEKIIFSGQFDGKIYQRKKSKYVWPPNVKYGTYRIQLDLPE
                     HDKKLAILFPHIFGAYRAYLNGKLITKVGEFSTKSENNKISFYTHTVPVDPGLKDAEL
                     IIHVANFSIRQTGFEKPLEIGTLSQLDQKKLQRTLIDLFIAGSLFYMGFYHLVLFFLR
                     KKEKTTLFLGLFCFVMFIRTLIFGERLLYQVSQGSSIIPAFEIMNYLSFYLGIPLLVE
                     FFYRLYPQEFKLIPVRVYQVIGLIAACTLFLQKTNVTFMVVQVYEILSLLIAFYGLFV
                     IILALKRKREDALLFFIGLGIFSLAGLNEALYNNGNINTFNTLGPGLFLFLYIQSYIL
                     ARRFSSSFVKNEMMKVELEKINLQLLSADKLKDEFLANTSHELRTPLIGIIGLTEALL
                     DGVTGPLSRETRNELKLIYINGKRLAMQVNDILDFSRLKNNDIALRCKDTDLQKCAEL
                     VLSLSRFTLGKKKIEFKNSIKEEFAHVFVDESRIQQVLNNLIGNAIKFTDEGVVEVTT
                     ELVLEENGKPMIALSVSDTGIGIAEEKQQEIFNTFVQADGSIIRKYGGTGLGLSITKR
                     LVELHGGNVSVCSKPGYGSCFTITLPVNKCDLPDDAVLDDDILDKKESYIPSDIDHYG
                     YTYNLQSIQTANDSVNSTTIMVVDDEPVNIHLLRNSLILEGYNVITADNGFDALQILD
                     EEAVPDLILLDIMLPKLSGYDVAKKIRERFNVSELPIIMLTAKSQVFDIVAGFDLGAN
                     DYVTKPFEKRELMARIKTLVSLKKSLKEREKLSLLENEMNLAQKVQGSIFASYKDWSK
                     IEKYDIAIQYVPQNSKVSGDYYHITQDSDNSLYLLVADASGHGVQAALRTAQIDVINR
                     ELGIIKDPATKMNQMNSILIERLDHGNYLTAFALNIIDNQAYFSAAGHTHQIVVRAKN
                     CELELIHTKGTLFGLSSNSVYTTKSVTIGEGDIIFLFTDGIFEQFGKSGKILGEEAFY
                     KFVKKTCSSKKGEPIEKITSTLVKMVTDYVGDRELNDDITCIGVRIKKHDVSKS"
     gene            complement(6138..>6609)
                     /locus_tag="P5123_09985"
     CDS             complement(6138..>6609)
                     /locus_tag="P5123_09985"
                     /inference="COORDINATES: protein motif:HMM:NF019559.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="lysoplasmalogenase"
                     /protein_id="HRX16634.1"
                     /translation="DFLLEGVTETHFLLGIAANMVAHVLYIIGFTVECKRLHTLRAII
                     PLVYISGLLSILLPNISGFMSWAVAFYAIIIGLMAWRAAAVIGQSGLNRYDEIIGLLG
                     AWLFITCDTLIAVDKFVVQILYAKYWIMTSYWIGQWCIAYSIIILFPGQKRARL"
BASE COUNT         2083 a         1146 c         1296 g         2084 t
ORIGIN      
        1 tatttattgc aagcgttgct ttcttcgtgt ttaacaccat ttttgagaca tcagattcaa
       61 ccgaggtgtc tacagaacat ttcagacttg ataatcatcg atatccactt atagataccg
      121 catcgggtga aagccggttt acttataaaa atgaaaatga tctgttaagt gattatgaaa
      181 aatcgcgtgg ctatattaag agtggtaatc ataatgatgc cttattgctc cttaatcgga
      241 ttatgcttag taacgttaat atatctgtta aagacagagt tgtgtttctt aaagatttta
      301 tacgtcgtgt cgaaaaaaaa agttacgaca aaattgattt cgcaaaactt aaagctgatc
      361 cagagcttta taacgggttt tatattatta tgtcaggcaa ggtagccaat tttaaaagta
      421 acgatgagtc ttcggccttt actcttttag ttaatgaaaa aaatgacaat tttgatgcag
      481 ttgctcaggt tataatggat ggcacaactt ctgttgaaaa cggagtttcc gttacagtca
      541 aaggcgagtt tttaaaatct gctgatgacg gaggcattat cattaaagct gataatgtta
      601 ttgccgacta aactgagatt ttgtttttct tgtaatataa tatttttcaa taataataaa
      661 ttaagagcaa aattattatt tttatttgtg atgtactata ctatttgtat gacccacggg
      721 gcctttccgt attgatcatt gataggagta aattatgccc tcgttaaaat ttcttggaac
      781 tggtacatcc catggtgttc ccgaaattgc ctgcagttgt gacatttgca catcgaccaa
      841 ctctaaaaac aggcggctgc gctcttgtgc gcttttacaa gagaatggac gtaatctcct
      901 tattgattgc tctaaagatt tcagagagca agctctggct cataaaatta ataatataac
      961 agatattctt gtaacacacg cacatgctga ccatgttttc ggtctggacg agcttcgtgt
     1021 ctataacaga cttcataaaa gtcgcataaa cctatatatg tcttcagagt gcgataatag
     1081 cttacggata cttttcccct atatttacgg gcagccgctt caaaaagggg gcggggtaag
     1141 tttgcttgat aaccatgtag tgcagcctgg tattgccttt gatgctgcgg gatttaatat
     1201 aatcccaata ccggttatgc acggtaagct cccgatttat ggataccgca taggtgacct
     1261 cgcatacatt accgactgct catatatcag cgaagagtcg tttacgcttg cacagggagt
     1321 aaaagttctt gtgttagggg cattgcgtta tagagaacat cccacccact ttaatttgga
     1381 tcaagcagtc gaagcggcac aaataattgg tgccgatcaa acctggtttg tccatatttc
     1441 gcacaaccta gatcatgata aagttaacag tgaacttcca gaaaatatta atcttgccta
     1501 tgacggactc accctgcggt tttagttgaa ttaatgatta atctcccaca atagatttga
     1561 gcattcttct tcttttagtc tgacttttac ccacaattat tatcttgcaa atgtcgagca
     1621 ttaggcccta taaattctcc aatggtaaaa ccttgagccc cctatatccc ccaatggggg
     1681 actttgaata gttgctacat agaatatgat ttttaatata aatataattt tttcattttt
     1741 catttttcac aattcattat tcattgtctt atcctgtaac gcaatgaact ttctgattga
     1801 catttttgga taacctttca taatttatca atgacatatt taagtttaaa atcggattat
     1861 gttttcaaaa agctcttcgg tgaagagccg gatattctta tggatctttt aaactcaata
     1921 cttgatttgc cttcggaaca gaccattaaa gagcttactg tattaaatcc cgaaatccca
     1981 aaagagcttg tgtcggagaa aacatcaatc tttgatatac gggcgcttaa tcagaatggc
     2041 gagcagatca ttatcgagat gcaggcattc ccacagtatt tttttgtgca gcgtgcactg
     2101 ttttactggg caaaaaccta cgcctcacag cttgaaaaag gtgataatta ccagcaattg
     2161 aagaaggtct attcaattaa tttcttggac tttaatctga tcgacacaaa agagtatcat
     2221 actgtatttg atattatttc aagggataac acggatatac agctaacaga agattttgag
     2281 atgcattttc ttgagatcaa aaagtttcct aatacaacag cctttactga caaactggat
     2341 atttggatgt atattatcaa taacactgag atgatagagg aaaatgtaat gaaaacaata
     2401 gtagataaac agccgttaat gaaacaggtt tttgacaggc ttgataagat gtctatgaac
     2461 aagactcttc taacagaata cgagatacgt aagcgagcgg ttatggatga aaaggcaaga
     2521 acacagtatt cgtataacaa aggtattgag gatggaattg agcaaggaat tgaaaaggaa
     2581 aaactccaaa cagcatcaaa gctgctggca aagggattca ctgtcgatgt aatatctgat
     2641 ttaacaggtc tttctactga taagattgaa gatttgcgat agatattaat ctctcaccaa
     2701 agatttgagc acccttctcc tcgaggagaa ggggcggcga cttgcacatg acgggctcac
     2761 tgtgcagttt taggtgagtt tatttctcag gatattttat ctattcttga caaaaattga
     2821 ttaagattaa aaaataacct atattaacag gcatatagtc aatttatgaa cgaccaccgg
     2881 ggaaaaccat gtggaatctt tatttgctaa gagtatttaa aaatcatttt ttcctgtcct
     2941 tttttatgcc attaatcctc ttattatcaa gttctaactg ttattcagca caagatccta
     3001 taacgcttga cctgacaatt caagacttgt catcaagatc ctttcaaatt gactctgatt
     3061 gggaatttta cccagaaaaa atcatatttt ccggacaatt tgatggtaag atatatcaaa
     3121 gaaaaaaatc taaatatgtt tggcccccga atgttaaata tggcacatac cgaattcaac
     3181 ttgatctacc agaacatgat aaaaagttgg caattttgtt tccacatata tttggcgcgt
     3241 atcgtgctta tttaaacggt aaacttatta cgaaagtggg cgaattctcc acaaagtctg
     3301 agaataataa aattagtttt tatacacaca ccgtgcctgt cgatccggga cttaaagatg
     3361 ccgaacttat aatacatgtc gcaaactttt ccattagaca aacaggtttc gaaaaacctc
     3421 tggaaattgg tactttatcg caacttgatc aaaaaaaatt gcaacggaca ttaattgatc
     3481 tttttattgc aggtagtctc ttctacatgg gtttttatca ccttgttctg tttttcttgc
     3541 gaaaaaaaga aaaaacaact ctgtttctgg gactgttctg ttttgtgatg tttataagaa
     3601 cgctgatttt tggtgaaagg cttctatatc aggtttctca aggaagcagc attattccgg
     3661 cttttgaaat catgaattac ctttcgtttt atcttggcat accactttta gttgaatttt
     3721 tctatcggct gtatccccag gagtttaaac tgatacctgt tcgtgtttat caggttattg
     3781 gtctgattgc tgcttgtact ctgtttcttc aaaaaacaaa tgttactttt atggttgtac
     3841 aagtttacga aattttgtct ttgctgatag ctttttacgg tctgtttgta ataattttgg
     3901 cgttaaagcg taaaagggaa gatgctcttc ttttctttat cggtcttgga attttctcat
     3961 tagcggggtt aaacgaagct ttatataata atggtaacat taatacgttc aatacacttg
     4021 gaccggggct tttcctattc ttgtatattc aatcctacat tctggcacga aggttctctt
     4081 cttcttttgt gaaaaacgag atgatgaaag ttgaacttga aaaaataaat cttcagttat
     4141 taagcgcgga taagcttaaa gatgaatttt tggcaaacac ttcacacgag cttcgtactc
     4201 ctcttatcgg gataataggt cttacggaag ctcttttgga cggcgtcacc ggtcctcttt
     4261 caagggagac aagaaacgag cttaagctta tttacataaa tggtaaacgt cttgcaatgc
     4321 aggtaaacga tattctggat ttttcaaggc ttaaaaacaa tgatatagca ttgcgttgta
     4381 aggatacaga tttgcaaaaa tgtgccgagc ttgtattgtc tttatctcgt ttcactctgg
     4441 ggaaaaagaa aattgaattt aaaaattcta taaaagaaga gtttgcacat gtatttgtgg
     4501 atgaatcgcg tattcaacaa gttcttaata atctgatcgg aaacgccata aagtttacgg
     4561 atgaaggtgt tgtggaagtg actacggagc ttgtcttaga agaaaatggt aagccaatga
     4621 ttgctctttc agtgtccgat accggtatag gtatagcgga agagaagcag caggagatat
     4681 ttaatacttt tgtacaggct gacggttcaa ttattagaaa gtacggtgga acagggttgg
     4741 ggctttcgat cactaaacga cttgttgaac ttcatggcgg taatgtatcg gtatgttcaa
     4801 aacccggata cggatcatgt tttaccataa ctttacccgt gaacaagtgt gatttgcctg
     4861 atgatgcagt tttagatgat gatattcttg ataaaaagga gagttatata cccagtgata
     4921 ttgatcacta tggatatacc tacaatttgc aatcgataca aacggccaat gattctgtta
     4981 atagtacaac aataatggtt gtcgatgatg aaccggtaaa tattcattta ttaaggaatt
     5041 cactgatact tgaggggtat aacgtaatta ccgctgataa tggttttgac gctctgcaga
     5101 tactggacga agaggctgtt ccggatctta tattgctgga tatcatgcta ccaaaactat
     5161 cgggttacga tgttgcaaag aaaatcaggg aaagatttaa tgtttcagaa ctgcctataa
     5221 ttatgctaac ggctaaaagc caagtttttg acattgtagc cggtttcgat ctaggtgcaa
     5281 atgattatgt taccaaacca ttcgagaaaa gagagttaat ggcaagaatc aagactcttg
     5341 tttcgctaaa gaagtcgtta aaagagcgtg aaaagcttag tttgcttgaa aatgagatga
     5401 atcttgcgca aaaagttcaa ggttcaattt ttgcttctta taaggattgg agcaaaattg
     5461 aaaaatatga tattgctatt cagtatgttc ctcaaaacag caaggttagc ggtgattatt
     5521 atcatataac gcaggatagc gataattctc tgtatctctt agtggcagat gcgtcgggtc
     5581 atggggtaca ggctgcccta agaactgccc agatagacgt aattaaccgt gaacttggta
     5641 taataaagga tccggctacc aagatgaacc agatgaactc aattttgata gaacgacttg
     5701 accatggaaa ttatctgact gcttttgctc tgaatattat agacaatcag gcgtatttca
     5761 gtgccgcggg tcatactcac cagattgtgg ttagggcaaa aaactgtgag ttggaactta
     5821 tccatacaaa gggaacacta tttggtcttt cttcgaatag tgtttatact acaaaatcgg
     5881 taacaatagg tgagggtgat ataatatttc tttttactga tggaatattt gaacagtttg
     5941 gtaaaagcgg gaagatactt ggcgaagaag ccttttataa atttgtgaaa aagacatgct
     6001 catctaaaaa gggagaaccc atagagaaaa ttacatccac tctggtaaaa atggtaaccg
     6061 attacgtggg cgacagagag ttaaacgatg acattacctg tataggtgta cgaatcaaaa
     6121 aacatgatgt ttccaagtca taatcgggct cttttttgtc ctggaaaaag aataataatc
     6181 gagtaggcaa tacaccactg tcctatccag tagctggtca taatccagta cttggcgtaa
     6241 agtatctgta caacgaattt gtcaactgcg atcaacgtgt cgcaagttat aaagagccag
     6301 gcgccaagta aaccgattat ttcatcataa cggttaagtc cgctttgacc tatcactgcc
     6361 gcggctcgcc atgccatcag accaataatt atcgcgtaga aagcaactgc ccaactcata
     6421 aagccagaaa tatttggtaa aagtatagat aataaaccgg agatatatac aagaggaatt
     6481 atagctcgta atgtatgaag tcttttgcat tcgactgtga acccgattat gtaaagaaca
     6541 tgagctacca tattggctgc tatacccagt aaaaaatgcg tctctgtaac tccctctagt
     6601 aaaaagtca
//
LOCUS       DAOTGZ010000158         6596 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_3064
            NODE_13813_length_6596_cov_5.452530, whole genome shotgun sequence.
ACCESSION   DAOTGZ010000158 DAOTGZ010000000
VERSION     DAOTGZ010000158.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868088
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 6596)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6596)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:22:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,785
            CDSs (total)                      :: 2,751
            Genes (coding)                    :: 2,732
            CDSs (with protein)               :: 2,732
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 12 of 19
            Pseudo Genes (internal stop)      :: 5 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6596
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13813_length_6596_cov_5.452530"
                     /isolate="AMR_MDS_3064"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..193
                     /locus_tag="P5123_09990"
     CDS             <1..193
                     /locus_tag="P5123_09990"
                     /inference="COORDINATES: protein motif:HMM:NF016629.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="Rpn family recombination-promoting
                     nuclease/putative transposase"
                     /protein_id="HRX16635.1"
                     /translation="NGEDITLIVPIIFYHGKQGWKIVPFKENFIGNSNNFYRFILDFD
                     YILTDIFRYDDEIKRRGQA"
     gene            165..335
                     /locus_tag="P5123_09995"
     CDS             165..335
                     /locus_tag="P5123_09995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004478271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRX16636.1"
                     /translation="MKLREEGKLEGKLEGKLKGITKGKLETAQKMLLKGFSVQEIVEI
                     TGLSEKDINSLI"
     gene            556..1341
                     /locus_tag="P5123_10000"
     CDS             556..1341
                     /locus_tag="P5123_10000"
                     /inference="COORDINATES: protein motif:HMM:NF014543.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRX16637.1"
                     /translation="MKKIILISLFFAVLSCGSTTSRLVEADMPKVVSEDGTEYIGDTK
                     NGMRHGKGILKYPGIQFYEGEWKENMRHGHGKQKYYDGREYEGMWVNDKMEGEGTLTF
                     ADKRVLKGIFKNGYVDGTSTVTLPDGSVYKGTVVKNRMEGHGEIVFSDKAVYKGEFSN
                     DRIHGEGEFRTADGRVYTGTFQNGTIHGKGKVVYTDGSVYTGTFRNNKIDGSGVMRYS
                     DGRVYTGSFRNDQMHGKGHMKYKNGRVVNGIWENGKLIRKLKK"
     gene            complement(1352..3925)
                     /gene="mutS"
                     /locus_tag="P5123_10005"
     CDS             complement(1352..3925)
                     /gene="mutS"
                     /locus_tag="P5123_10005"
                     /inference="COORDINATES: protein motif:HMM:TIGR01070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0030983 - mismatched DNA binding [Evidence
                     IEA];
                     GO_process: GO:0006298 - mismatch repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair protein MutS"
                     /protein_id="HRX16638.1"
                     /translation="MDTKLTPMLRQYLDIKKQHPNEILFFRMGDFYEMFFEDAHTASS
                     ILSIALTKRQNDVPMCGIPFHAADSYVSRLIKAGHRVAICEQLETVPSEGKIVKRDVV
                     RIITPGTVVESHLLGSDENNFLAGIVFGKESIGLAFADVSTGDFYHTEITRSFDIFKG
                     ILARFTPREIIMKSFENPDDKKFTEYIDLLEIPLNRINDWLYDKQYLEKTIKDIFKVS
                     SLSGLSLESELSILTAGSILEYIKIAHKRSIEHLKLPRIINSSSHMMLDDATINNLEL
                     ITNAYDRSRSKTLFAVLNRTKSPMGKRLLERTILQPLTDKSEIEERLDCVQYFFEYHE
                     FAQSTVSVIKNINDIERLLSRFSMGKIVPKDFLALRNTILGCEELKALFKKHQESPLY
                     PISQNIIAPVKLAEHIAKAILEEPALSPEHGRVIAEGFNSELDSLHDINRDSRKWILE
                     YQEKEKQALGINTLKIKYNRVHGYYIEVSKSSTNKIPENYLRKQTLVNAERYTTDVLQ
                     QLETKILSASDKIVEIEKKIINEIHSEVLKNRELLQKSASAIAEVDLYLSFAETARAE
                     RYVRPEFEEGRRTEIVNGRHPVVEKYFTNEVFIPNDVLFDGDDNQLKIITGPNMSGKS
                     TYMRMCAVVQLMAQIGSFVPADLAKLSIVDRIFTRIGASDNISRGESTFLVEMNETAT
                     ILNNATDKSLIIMDEVGRGTSTYDGLSLAYAIVEYIATYIKARTLFATHYHELTELER
                     LNGIVNYNVMVQETVRGVDFLHKVVKGAADKSYGIHVAKLAGIPKEITMKAASILKKL
                     ESRKSDKKKTAEPTNANEQVELFNAANHIIIKTLESIDTDAITPIEALNELNRLKNLL
                     S"
     gene            complement(3969..5123)
                     /locus_tag="P5123_10010"
     CDS             complement(3969..5123)
                     /locus_tag="P5123_10010"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRX16639.1"
                     /translation="MGLLFTVKKFRIWTLFLFLMLLLAAPLVASSEHNDYIKIIESAY
                     SDDYGRISWLCRSFYSKYPKSRLIPEIRIIESRTLRDSNAMIKSLKDIIKKYPTFKDN
                     DQCQLKICEILYLSARWSELDREATIAISVYGKRNRFYPFFIHYRSKAAFYTHNYEAG
                     IGFADNAFEADKNFVFYPELRLQNIFSEQKIHYDGDRYVSQLLESYYELEGSGTEVSS
                     LYLLARNSELKNQHSLAYSIYTDLIKRYPRSPEALMAANRISIMKDFKPRYIKNPLAK
                     LDKQKPNIIDQLSPDTPVEKAKFKSYYALSIGPFYNLQEAEKLMKQLKRDFDSVFIIR
                     GYNTFSIYVGQSESSENAMHYKIRLAEEFGINGDIVYIASEENLKFIHKE"
     gene            complement(5092..6423)
                     /gene="miaB"
                     /locus_tag="P5123_10015"
     CDS             complement(5092..6423)
                     /gene="miaB"
                     /locus_tag="P5123_10015"
                     /EC_number="2.8.4.3"
                     /inference="COORDINATES: protein motif:HMM:TIGR01574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016782 - transferase activity,
                     transferring sulphur-containing groups [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (N6-isopentenyl
                     adenosine(37)-C2)-methylthiotransferase MiaB"
                     /protein_id="HRX16640.1"
                     /translation="MNNKNKLPFLSYFIETFGCQMNISDSELIAASLENEGLFKMNMI
                     DADVVVFNTCSVRKHAEDRAVARLREAKAHKRKRGIVIAAGCLSQHIGTRLLEDKICD
                     IAVGPYESPNMTQIIREYIKDNSRNLFISSEKNDFAGRLHPSLVMRPERESWHRFVTI
                     THGCENFCTYCIVPHVRGPLISFNSEEIIDFIKQLPRNGVFEVTLLGQNVNQFGQDSG
                     DISFCQLLEQVSTIPGIEKINFMTSHPKDFNPDIIRQIANNSTIARSIHLPLQSGSDD
                     ILRRMNRGYNVKHYMGLINSIRQAGDIAITTDLIVGFPGETEEQHKESMSLIKEIGYD
                     DAYMYAYSPREGTVSAGYDMQLSKETKNRRLTELITMQREITKSRLNKLIGKKEKIVV
                     ERVSRKNDNEVSGKTFSNRQAVLAGSSDDIGKIFTIQYEKVNGATLYGKKI"
     gene            complement(6416..>6596)
                     /locus_tag="P5123_10020"
     CDS             complement(6416..>6596)
                     /locus_tag="P5123_10020"
                     /inference="COORDINATES: protein motif:HMM:NF021883.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="adenylosuccinate lyase"
                     /protein_id="HRX16641.1"
                     /translation="MVQTNAMRAWGGEGSLKDLVLHDSEITALLSNEEIDNIFDINKY
                     FENIQAVYAKTGIDE"
BASE COUNT         1892 a         1204 c         1339 g         2161 t
ORIGIN      
        1 aaatggggaa gatattactc tgattgtgcc aataatattt tatcatggta agcaagggtg
       61 gaagatcgtt ccgtttaaag aaaattttat aggtaattca aataattttt acaggtttat
      121 tctagatttt gattatattt tgactgatat ttttcgctac gacgatgaaa ttaagagaag
      181 agggcaagct tgagggtaaa cttgagggta aactgaaagg tattacaaaa ggaaaacttg
      241 aaacagctca aaagatgctt ttgaaaggtt tctccgttca ggaaattgta gagattacag
      301 gtctttctga aaaagatatt aattctttga tatagcccaa aagattgcgt agtcttttgg
      361 tgcattacgg acaatttctg gccaatgtca attaaacgca acttttagtc ggttactagc
      421 tttgcacatt cgttataggc tttcggttaa ttcttcttta aaacaactta attcatgcct
      481 tcagattctg tttataaaat ccgatattta tatggtgtac ccggttttgc cggcattatt
      541 tgatggtgtg attatatgaa aaaaataatt ttaatatctc ttttttttgc tgtattatcc
      601 tgcggttcaa caacgtcaag gcttgttgaa gctgatatgc ctaaggttgt atctgaagat
      661 ggtacagagt atattggaga tacaaagaac gggatgcgcc atgggaaagg tattcttaag
      721 tatcctggaa ttcagtttta cgaaggcgag tggaaagaaa atatgcggca tggtcatggg
      781 aagcaaaaat attatgacgg tcgtgaatat gaaggaatgt gggtaaatga taaaatggaa
      841 ggtgagggta ctctcacttt tgccgataag cgtgtcttaa agggaatatt taagaatggc
      901 tatgttgatg gaacttcaac tgtgacttta cctgacggat cagtttacaa aggaactgtt
      961 gtaaaaaacc gtatggaggg gcacggtgaa atagtctttt cggacaaggc tgtttacaag
     1021 ggagaatttt caaacgatcg tattcatggt gagggagagt ttcgcactgc agacggacga
     1081 gtttacacag gaacctttca gaatggtaca attcatggaa aaggcaaggt tgtttataca
     1141 gacggatctg tttatacggg gacttttcga aacaacaaga tagacggaag cggagttatg
     1201 cgttacagcg atggtcgtgt gtacaccggt tcttttagaa acgatcagat gcacggaaag
     1261 ggacacatga aatataaaaa tggccgggtt gtgaacggta tctgggaaaa cggcaaactt
     1321 atacgaaaat taaaaaaata gaagtaattt tttacgataa aagattttta agtctattaa
     1381 gttcattcaa ggcttcgatt ggtgttatgg cgtccgtgtc tatgctttcc agtgttttaa
     1441 taataatatg atttgctgcg ttaaaaagct ctacctgctc gttagcatta gttggctcgg
     1501 ctgttttttt cttgtcactt tttcgggact caagcttctt taaaattgaa gcagctttca
     1561 ttgtaatctc tttgggaata cctgccagtt tggctacatg gattccataa gatttatcag
     1621 cagctccttt tacaaccttg tgtaaaaaat cgacaccgcg tacggtttct tgcaccatta
     1681 cgttataatt aacaattccg ttaagtctct caagctcggt cagttcatgg tagtgggtag
     1741 caaaaagtgt cctggccttt atataggtcg caatatattc cacaatggca tatgccaaag
     1801 atagtccatc gtaggtagaa gtcccccggc cgacttcgtc cataattata aggcttttgt
     1861 ctgtagcatt attcagtatt gttgcagtct cattcatctc aaccaaaaaa gttgattcac
     1921 cacgcgaaat attatcagac gcacctatac gtgtaaaaat tctatccaca atagagagtt
     1981 ttgcaaggtc ggcgggcaca aatgatccaa tttgagccat tagttgaact acggcacaca
     2041 tccgcatata agtcgattta cccgacatat taggaccggt gataattttt agttggttat
     2101 cgtcgccgtc aaagagaaca tcgttgggta taaaaacttc gtttgtaaaa tacttctcga
     2161 caacaggatg tcttccgttt actatttcag ttcgtcttcc ttcttcaaat tctgggcgta
     2221 catatcgttc tgctctagcg gtttcggcaa aagaaagata caggtcaact tcggctatcg
     2281 ccgaggcact tttttgcaga agctcgcggt tttttaatac ttcactgtgt atctcgttta
     2341 taatcttttt ttctatttca acaatcttgt cagacgccga caatatcttt gtttcaagct
     2401 gttgaagtac atcggttgtg tatctctcgg cgttaaccaa agtttgtttg cgaagatagt
     2461 tttctggtat tttgttggtc gaagatttac ttacttcaat atagtatccg tgaacacgat
     2521 tgtacttaat ctttaaggta ttaattccaa gtgcctgttt ttctttctcc tgatactcca
     2581 atatccattt gcgcgaatcg cggtttatat cgtgaagaga gtccagttca ctgttaaaac
     2641 cttcagcgat aactcgtcca tgttcgggtg ataaagctgg ctcttctaag atcgcttttg
     2701 cgatatgttc tgcgagttta actggggcaa taatgttttg agaaatgggg tatagggggc
     2761 tctcctgatg ttttttgaaa agagctttta attcttcgca accaagaatg gtgtttctta
     2821 gtgccaaaaa atcttttggt acaattttcc ccattgaaaa tcttgaaaga aggcgttcaa
     2881 tgtcgttaat attttttatt actgatacgg tcgattgcgc aaactcatgg tattcgaaaa
     2941 aatattgaac acaatcaagc cgttcttcaa tctcgctttt gtcagttagg ggttgaagta
     3001 ttgttcgttc aagaagcctt ttccccatag ggcttttggt tctgtttaaa acagcaaaaa
     3061 gtgttttaga acgagacctg tcgtacgcgt ttgttatcag ttcaagatta tttatagtag
     3121 cgtcatcaag catcatatga gacgagctgt ttattatgcg tggaagtttg aggtgctcaa
     3181 ttgatctttt gtgggcaatt ttaatatact ctagtattga tcccgctgtt aagattgaga
     3241 gttcgctttc aagagagagg ccggaaagcg agcttacttt gaagatatct ttgatggttt
     3301 tctcgagata ttgtttgtca taaagccagt cgttgattcg gtttaatggt atttcaagaa
     3361 gatcaatata ttcggtaaat tttttatcat caggattctc aaagctcttc ataattattt
     3421 cgcgcggtgt aaatcttgct aaaattcctt tgaatatatc aaaagaacgg gtaatctcgg
     3481 tatgataaaa atctcctgtc gaaacatcag caaatgctag accaattgat tcttttccaa
     3541 aaacgattcc ggccaaaaaa ttgttttcat cagaaccaag aagatgcgat tcaacaactg
     3601 tgcctggtgt tataattcgt acaacatcac gtttaacaat cttaccttcg gatggaacag
     3661 tctccaattg ttcacaaatt gcaacacgat gcccggcctt gatcaatcgg gaaacataac
     3721 tgtccgctgc atgaaaaggt attccacaca taggaacatc gttttgtctt ttggttagag
     3781 caatgcttag tatggatgac gcggtatgag cgtcttcgaa aaacatctcg taaaaatcac
     3841 ccattctgaa aaacagtatc tcgtttgggt gttgtttctt gatatcaaga tattgtctaa
     3901 gcattggtgt aagctttgta tccattgaaa cctcaaatga tttttgtaaa aattcaaatt
     3961 aattccggtt actctttatg aataaatttt agattttcct ctgaagcgat atacacaatg
     4021 tctccgttaa taccgaactc ttcagcaaga cgtattttgt agtgcattgc gttttcagat
     4081 gattcgcttt ggccaacata aatggagaag gtattgtaac cacgtataat aaaaaccgaa
     4141 tcgaaatccc gtttaagctg tttcatcaat ttttccgctt cctgaagatt ataaaaaggg
     4201 cctatcgaca atgcgtaata acttttaaat ttggcttttt ctacaggagt gtccggcgat
     4261 aactgatcga taatatttgg tttctgttta tcaagtttgg caaggggatt ttttatatat
     4321 ctgggtttaa aatctttcat tatggaaatg cggttcgctg ccataagggc ttcgggagat
     4381 cggggatatc tcttaataag gtctgtatat atcgagtatg cgagcgagtg ctggttttta
     4441 agctcactgt ttctggccaa aagataaagg gaagatacct cagtgccact gccctctagc
     4501 tcataatatg attccaaaag ttgtgataca taacggtcgc cgtcgtagtg tattttctgt
     4561 tcagaaaata tattttgaag tctaagctcg gggtaaaaaa caaagttttt gtcggcctca
     4621 aaggcgttat cagcaaaacc gatacctgct tcatagttat gtgtgtaaaa cgcggctttg
     4681 gatctgtagt gaataaaaaa cgggtaaaac cggtttcttt ttccataaac agatattgca
     4741 atggtcgctt ccctgtccag ttcgctccac ctggccgaaa gataaagaat ttcgcaaatt
     4801 ttaagctggc actggtcatt atctttaaaa gtggggtatt tttttataat atctttaagc
     4861 gattttatca tcgcgttact gtcacgaaga gttctgctct cgattattct tatttcaggt
     4921 attaacctgc ttttagggta tttgctgtaa aaagaacgac agagccacga gatacggccg
     4981 tagtcatcag agtaggcact ttcaataatt ttaatatagt cattatgttc agaagacgca
     5041 accagtgggg ctgctaaaag cagcataaga aacaaaaaaa gagtccatat cctaaatttt
     5101 tttaccgtaa agagtagccc catttacctt ctcgtattgt atggtgaaaa ttttaccgat
     5161 atcatcactg cttccggcga gtacagcctg ccgattcgag aatgttttac cggatacctc
     5221 attatcattt tttcggctga ctctttcaac aactattttt tcctttttcc caatcaattt
     5281 attaaggcgt gatttggtaa tctctctttg cattgtaata agttcggtta aacgcctatt
     5341 tttagtctct ttggaaagct gcatatcata gccggcgctt acggttcctt ctcttggaga
     5401 gtaggcatac atataagcat cgtcgtatcc aatctctttt atcaaggaca tgctctcttt
     5461 atgttgttct tcggtttctc cgggaaaacc aacaataaga tcggttgtaa tggcaatatc
     5521 cccagcctga cgtatcgagt ttattaatcc catatagtgt ttaacattat aaccacggtt
     5581 cattcttcga agaatatcgt cagacccgct ctgaagaggc aggtgaatcg agcgcgcaat
     5641 ggtgctatta tttgcaattt ggcgtataat atcaggatta aagtccttgg gatgagatgt
     5701 cataaagtta attttttcta taccgggtat tgttgatacc tgctctagaa gttggcaaaa
     5761 acttatatca ccactatcct gaccaaactg gtttacgttt tgaccaagaa gtgttacttc
     5821 gaatactccg tttcgcggaa gctgttttat aaaatcgatt atttcttccg agttgaaaga
     5881 tataagcggt ccccttacat gcggaacaat acaatatgta cagaagtttt cgcatccatg
     5941 tgttatggta acaaatctat gccaagattc cctttcggga cgcattacca gagaggggtg
     6001 aagtctaccg gcaaagtcgt ttttttcaga ggagataaac aagttgcggc tgttgtcttt
     6061 tatatactcc cgaataattt gtgtcatatt gggtgattca taagggccaa cggcaatatc
     6121 gcaaatttta tcctctaaaa gtcttgtgcc gatatgctga gaaagacaac cggcagcaat
     6181 aacaataccc cgttttcgct tatgagcctt cgcctctctt aaccgcgcta cagcccgatc
     6241 ttcggcatgt ttgcgcaccg aacaggtgtt aaatacaaca acatcggcat caatcatatt
     6301 catcttgaaa agaccttcgt tttccaaaga tgcggctatc agttcactat cgctgatatt
     6361 catctggcaa ccgaaggttt cgataaaata gcttaaaaaa ggtaatttgt ttttattatt
     6421 catcaattcc ggtctttgcg tatacagcct gtatgttttc gaaatattta ttaatatcaa
     6481 atatattgtc gatctcttca ttacttaaca gtgcagtaat ctcggaatcg tgtaaaacaa
     6541 gatctttaag agaaccttct ccgccccatg cgcgcattgc gtttgtctga accatt
//
LOCUS       DAOTGZ010000159         6537 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_3064
            NODE_13989_length_6537_cov_4.546745, whole genome shotgun sequence.
ACCESSION   DAOTGZ010000159 DAOTGZ010000000
VERSION     DAOTGZ010000159.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868088
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 6537)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6537)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:22:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,785
            CDSs (total)                      :: 2,751
            Genes (coding)                    :: 2,732
            CDSs (with protein)               :: 2,732
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 12 of 19
            Pseudo Genes (internal stop)      :: 5 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6537
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13989_length_6537_cov_4.546745"
                     /isolate="AMR_MDS_3064"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1758
                     /locus_tag="P5123_10025"
     CDS             <1..1758
                     /locus_tag="P5123_10025"
                     /inference="COORDINATES: protein motif:HMM:NF024821.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HRX16642.1"
                     /translation="NPNLLTGIRLYNAGNYPDAISSFYEVVEGDTSDEEKSEALLYLG
                     RIEIISENYTKGVDYFKRALKFSDKNHNAYLEIARAYTGLNDLDRAIEYIDRAIEKRP
                     DLDDAHTAKGNILFKQKKFKAASDAYRHANKINPDNAYAHYNRALALLKIGERVEAME
                     ELKLASTSGQGRIAYLAYSRIGSLSLEENSIEQAIKYLNLALNLDATSPEDLFNLGLA
                     YLKKGDKDRAIDYFAKAEQAGTDDMVLLEGLAEVYSVSYNNYNKSIDLYKRVAEKNSR
                     NVLILKKLGELYYKSRDLNAALVYFKRVTEMEPASENARQAYLYMGTIYDENERHDDA
                     VEAYKSALDINPRDDETLYFLGQSYKLSGDTINAISTWRKAYSVNPDNPRPLIAMADL
                     LHSTGHLDEAQSEYSKIAEKWPANSEALFSLATILNKKGNSDDAAKYYKQVADLETDK
                     ELARKSLINLALLSADKEGLSSSEEGVSDSVVHIKKALMYDPGNTETLYALGIIYYKK
                     QLYDQAVESFYQVIKGSRDNQETARAYNYIGKSYFKKEDYKKAVRAFSMGIEEDPANE
                     EIRINRRAASDKYEEAISE"
     gene            complement(1768..3708)
                     /gene="priA"
                     /locus_tag="P5123_10030"
     CDS             complement(1768..3708)
                     /gene="priA"
                     /locus_tag="P5123_10030"
                     /inference="COORDINATES: protein motif:HMM:TIGR00595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005658 - alpha DNA polymerase:primase
                     complex [Evidence IEA];
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0003688 - DNA replication origin binding
                     [Evidence IEA];
                     GO_function: GO:0016818 - hydrolase activity, acting on
                     acid anhydrides, in phosphorus-containing anhydrides
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="primosomal protein N'"
                     /protein_id="HRX16643.1"
                     /translation="MNYADVFLPLPQDEFFTYIIPDNLRVTAGIRVTVPFGKRVLTGA
                     VFRTHNKKPTGFQLKTISDVLDDSPVFSDKFIELCSYTADTYCASRGEVFSMALPGRK
                     SGTSRYKEPFSTEPYQFKLSEAQQKICDTILNTGSGAHLVYGVTGSGKTELYMDLARS
                     AIGMGKTVLYLVPEITLSSQLHQRLYSVFGKDLAVYHSGLTQIQRADHWKQFYSGEKK
                     IIVGTRSSIFLQSDNLGLIIIDEEHDSSYKENSSPRYSAKRLALYRMKLENCTVVLGS
                     ATPSVESFFAAKKGVLHYHELKERFGNAALPEIVTVDLAESKDGISTALRVETAQAVR
                     RGEQVVYLLNRRGYSPVLLCGNCKKRVECPDCCVSLNYHSDGLMRCHYCGFSTALPKK
                     CPSCGSDDLNKIGSGTQRIEEQVESLFPTLRVFRLDRDSSRKKGTIVELIDMINNKEV
                     DIVVGTQMIAKGFDFHNITLVGILMADIGMNLPDFRSSERTFSLLMQVAGRCGRGTKP
                     GKVVIQTLDPGNYLFDFVRSQNYVAFYEHEIKLRKLMNYPPFTRIARLVARGKDEPAV
                     IKGVERVASFLKTAFGLTDGIQLLGPAPAPFTRLGGNYRYHIIIKFKNNALVKEKLSE
                     LNRVCKTKDVYIEIDIDPVELL"
     gene            complement(3705..4400)
                     /locus_tag="P5123_10035"
     CDS             complement(3705..4400)
                     /locus_tag="P5123_10035"
                     /inference="COORDINATES: protein motif:HMM:NF015146.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil-DNA glycosylase family protein"
                     /protein_id="HRX16644.1"
                     /translation="MYNQLREILSNDPSSILLYKNNDEYDYLLDWLSREDNVNQNISL
                     PKPATCSKGRSADVAKNTATVFDNVSTCTACPGIIERKKSVGDGSSGVMIILNAPIMM
                     SSFEKNELRGESVEILKKIVASMNLPLPTVYITNMIKCESSDPLLKPSTQLQSCLHFL
                     EDEIEQICPSVVIVMGDLTPLRRTRKKFINSSWFEIPHPLTLIKNPPLKREAWNTLQL
                     IMSVLEELKENKL"
     gene            complement(4403..5755)
                     /locus_tag="P5123_10040"
     CDS             complement(4403..5755)
                     /locus_tag="P5123_10040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011342106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54 dependent transcriptional regulator"
                     /protein_id="HRX16645.1"
                     /translation="MAKVLIVDDEPNILKVLSQILQDEQHIVYEASDGASAQDFIAKN
                     DIDLAFVDLWLPDIDGLEILECIKSSQPDAAVIMISGHASIDVAVKATKMGAYDFMEK
                     PPAYDRIVTITRNALETTMLRKENRTLKTQGDDGEMIGLSPALTEIKETIDRAASTNA
                     RVFITGPNGSGKELVARAIYNKSKRSGRPFVKVNCAAIPDELIESELFGHEKGSFTGA
                     HAKRLGKFEAANGGTIFLDEICDMSLSAQAKVLRVLQESEFERVGANETIKVDVRVIA
                     ATNVDIQKAIEENRFREDLYYRLNVIPIYVPPLIERTSDIPLLVDHYLRIFSNEHGIG
                     DKSISESGMLFLKSYPWPGNVRQLKNIIERVAIMVPDDTINESDIEKYIESADTFHDL
                     PLTEKSSLKKAKEQFEKEFIIKTLNSLNMNVTMAAKELGIERTNLHRKIKQYNIDTSR
                     "
     gene            complement(5766..>6537)
                     /locus_tag="P5123_10045"
     CDS             complement(5766..>6537)
                     /locus_tag="P5123_10045"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HRX16646.1"
                     /translation="LYKTEISDEISVLYNSFNVMVKQLDESRRAMMHMQKLEAWKEVG
                     SKLMHEIKNPLTPIRLSAERIRRKYFEQSDDIENIIKTGTDTIIEEVDALQHILREFS
                     RYTRLPKLNPVFQNINDILKNCVDFFHGHENIVFKLFLDDELPDLRIDRILFRQVLIN
                     IIKNAIEAMDYYGEIVIASQIENGKAIIRVRDNGPGIPPEDLGRLFEPTFSRKQEGTG
                     LGLAIVEKIVMEHGGKIICRSVPGNGAEFILTLPIYNR"
BASE COUNT         2007 a         1432 c         1221 g         1877 t
ORIGIN      
        1 aatccgaacc tactaacagg gataagactc tataatgcag gaaactatcc agacgcgata
       61 agctcttttt atgaagtggt agaaggagat acttctgatg aagagaaatc ggaagctcta
      121 ctctacctag gcagaataga gattataagc gaaaattata caaaaggagt ggactatttc
      181 aagcgtgctc tgaaattcag cgataagaat cataatgctt atcttgaaat agcaagggct
      241 tatacaggcc ttaacgacct tgaccgagcc atagaatata ttgaccgcgc catagaaaag
      301 aggcccgatc tggacgacgc gcacacagca aaaggaaaca ttctttttaa gcagaaaaaa
      361 ttcaaagctg cttcagacgc ctaccgtcac gcaaacaaga taaatcccga taacgcttac
      421 gctcattata accgtgcact tgcactatta aaaattggag aacgggtcga agccatggaa
      481 gagcttaaac tggcctcgac atccggtcaa gggagaatag catatcttgc atactcacgc
      541 atcggatcgc tatccctaga agagaacagc atcgagcagg caatcaaata ccttaacctg
      601 gcactaaacc tggatgccac ctctcccgaa gatcttttta atctgggtct ggcctatctc
      661 aagaaagggg acaaagaccg cgctattgat tattttgcca aagcggaaca agcaggaacc
      721 gatgatatgg ttttacttga aggtttggcc gaagtctact ctgtcagtta taataactac
      781 aacaagagta tagaccttta caagcgtgtt gcagaaaaaa atagccgcaa tgttcttata
      841 ctaaaaaagc ttggggaact ctattacaaa agccgtgacc taaatgcggc actggtctat
      901 tttaaaagag ttacagagat ggaacccgct tcagaaaatg cacgccaggc atatctttat
      961 atgggaacta tttatgacga aaatgaacgc catgatgatg ctgtcgaagc ctacaagagt
     1021 gccttggata taaaccctag agacgacgag accctctatt ttcttggtca atcatataaa
     1081 ctaagtggtg acactatcaa tgctatcagc acctggcgta aagcctactc cgttaatccg
     1141 gataatccac gccctttgat tgccatggct gaccttctcc attctacagg tcatctggat
     1201 gaagcacaaa gtgagtattc aaaaatcgct gaaaaatggc ctgctaatag cgaagcactg
     1261 ttttcactcg cgacaattct taacaaaaaa ggtaatagtg atgatgcggc aaaatattac
     1321 aaacaggttg ccgatcttga aaccgacaaa gagctggcca gaaaatcgct tataaacttg
     1381 gctctcttat cagccgataa ggaggggctc tcttccagcg aagagggagt atctgattcc
     1441 gtagtacata ttaagaaggc acttatgtac gatcctggaa ataccgagac cttgtacgcg
     1501 ctgggaatta tttattacaa gaaacaacta tatgatcaag ctgtcgaatc tttttatcag
     1561 gtaataaaag gcagccgtga caaccaagaa accgcccgtg cttataacta cataggaaaa
     1621 tcatatttca aaaaagaaga ttataaaaaa gcagtaagag cattttcgat ggggatagaa
     1681 gaagatccag caaatgaaga gataagaata aatcgacgcg cagcaagcga taaatatgaa
     1741 gaagcaataa gtgaataaaa gattcaatta caaaagttct accgggtcta tatcaatttc
     1801 gatatagaca tccttggttt tacaaaccct gttcagctca gaaagctttt cttttaccaa
     1861 tgcgttattt ttaaatttaa tgataatatg gtatctataa ttaccaccta aacgtgtaaa
     1921 tggtgctggt gctgggccaa gcaactgaat gccatcggtt aatccgaagg ctgtctttaa
     1981 aaatgacgca actctctcta ccccctttat tacagcaggc tcatccttac cgcgagccac
     2041 cagtcttgcg attcgtgtaa atggagggta attcataagt tttcgtagct ttatctcgtg
     2101 ctcatagaaa gccacataat tttgacttcg cacaaaatca aaaagataat taccgggatc
     2161 aagtgtctga atcactacct tgcccggttt ggtccctcgc ccacatctgc cggcaacctg
     2221 cattagaaga gagaatgttc tctccgatga tctaaaatcg ggcaaattca tgccaatatc
     2281 tgccatcaat atacccacaa gtgtaatatt atggaaatca aatcctttgg caatcatctg
     2341 tgtgccaaca acgatatcca cctctttatt gttgatcata tcaatcagct ccacgattgt
     2401 ccctttttta cgagagctgt cgcgatcaag gcggaaaaca cgcaacgtcg gaaaaagcga
     2461 ttcgacctgt tcctctattc tctgggttcc gctgccaatt ttatttaaat catctgatcc
     2521 gcacgaaggg cacttttttg gtaatgcggt agaaaagcca caatagtggc atctcattaa
     2581 accatcgctg tgataattaa gactcacaca acagtcagga cactcgactc tttttttaca
     2641 gttgccacaa agaagtaccg gagaataacc ccttcggttt agcaaataaa ctacctgctc
     2701 gcccctccga acagcttggg ctgtttcgac tcttaatgct gtcgatatgc catcttttga
     2761 ttcagccaaa tcaaccgtca caatttcagg caaggctgca ttcccaaaac gttctttaag
     2821 ttcatgataa tgtagaacac ccttttttgc cgcgaaaaag ctctctacag aaggggtagc
     2881 cgatccaagt acaaccgtgc aattttcaag cttcattcga tatagagcca atcttttcgc
     2941 actatagcgc ggagatgaat tttccttata agatgaatca tgttcttcat caataattat
     3001 cagaccaaga ttatcagact gtaaaaaaat ggaagatctg gttcctacga ttatcttctt
     3061 ttcaccacta taaaactgtt tccagtgatc tgcacgctga atttgagtaa gaccgctatg
     3121 atagacagca aggtcttttc caaatacgga ataaagtcgt tggtgaagct gggaagagag
     3181 ggtaatttcg ggtacgagat aaagtacagt ttttcccata ccaattgctg atctggcaag
     3241 atccatgtac aattcagttt ttccgcttcc ggtaacaccg taaacaagat gtgcaccact
     3301 accggtattt aatattgtat cgcatatttt ctgctgagct tctgaaagtt taaactgata
     3361 cggttctgtt gaaaaaggct ctttgtaacg gctagttcca cttttccgac ctggtaaggc
     3421 catcgaaaaa acctctccgc gagacgcgca ataggtatca gccgtatacg aacaaagttc
     3481 tataaattta tccgagaaaa ctggagaatc atctaaaaca tcagaaatgg ttttcaactg
     3541 aaacccggtc ggctttttgt tatgagttct gaaaacagcc cctgtaagga ctcttttacc
     3601 aaaaggtaca gtaaccctaa taccagcagt aaccctcaga ttgtcaggaa taatataggt
     3661 gaaaaactca tcctggggaa gaggcaaaaa aacatcggca taattcataa tttattttct
     3721 ttgagttctt caagtacaga cattataagc tgtaacgtat tccaggcctc ccgttttaac
     3781 ggcgggtttt tgatcaaagt caggggatgg ggtatttcga accagctaga attgataaat
     3841 tttttgcgtg ttcgtctcag cggcgttaga tcccccataa ctattactac agaggggcat
     3901 atctgttcga tctcgtcctc aagaaaatgc aggcatgact gaagctgagt acttggtttc
     3961 aatagtgggt cagaactttc gcatttaatc atgttggtta tatatacagt cggaagcgga
     4021 agattcattg aagccactat tttttttagt atctcaacag actcaccacg caattcgttt
     4081 ttttcaaaac tactcatcat aattggagca tttagtatta tcataacacc gcttgaacca
     4141 tcacccacac ttttttttct ttcaataata ccagggcatg cggtacaggt tgatacgtta
     4201 tcaaataccg ttgctgtatt ctttgctaca tcggcggaac ggcctttact gcatgttgca
     4261 ggttttggca gcgaaatgtt ttgattcaca ttatcttcac ggctaagcca atctaaaaga
     4321 taatcatact catcgttatt cttatacagc aaaattgagg aggggtcatt tgataatatt
     4381 tcacgcaatt ggttgtacat aactaccgcg atgtatctat attgtattgt ttaatttttc
     4441 ggtgaagatt ggtacgttca attccaagct ctttggcagc catggtaaca ttcatattga
     4501 gggaattaag agtttttata ataaactctt tttcaaactg ctccttagct ttctttaaag
     4561 aagatttttc cgttaaaggc aagtcatgaa aagtatcggc cgattctata tatttctcta
     4621 tatcagattc attaattgta tcatcgggca ccattatcgc cactcgttcg ataatgtttt
     4681 ttaactgcct tacgttaccc ggccaaggat aactctttaa aaatagcatt ccagattcac
     4741 ttatactttt atcaccaata ccatgctcgt tagaaaatat tcttaaataa tgatcaacta
     4801 gcagaggaat atcggatgta cgctctatca aaggaggtac ataaatagga ataacattaa
     4861 gtctgtaata aagatcctct ctgaaacggt tctcttcaat tgccttttga atatcaacat
     4921 ttgttgcagc aattactctt acatctacct tgattgtttc attagcccca actctctcaa
     4981 actctgactc ttgcaaaacc ctcaatactt ttgcctgcgc tgaaaggctc atatcacaaa
     5041 tctcgtcaag aaagatggta cctccgttag ctgcctcaaa tttaccaagc ctcttcgcat
     5101 gagcaccagt aaaggatccc ttctcgtgtc cgaaaagttc actttctatt aactcatctg
     5161 gaatcgccgc acagtttacc ttaacaaacg gacgacccga gcgtttggac ttattgtata
     5221 ttgctctggc aacaagttcc ttaccgctac catttggccc agtaataaaa accctcgcgt
     5281 ttgtagatgc agcgcgatca attgtctctt taatttctgt aagagctggg gaaagaccaa
     5341 tcatctcccc atcgtcaccc tgtgtcttta gagtacgatt ctctttacga agcattgtcg
     5401 tttcaagagc gttacgggta attgttacta tccggtcata tgcgggcggt ttttccataa
     5461 aatcatacgc gcccatcttg gttgccttaa cagccacatc gatagaagca tgacccgaaa
     5521 tcataataac agccgcgtca ggctggctgc ttttaataca ttcaagtatt tcaaggccat
     5581 ctatatcagg aagccataga tcaacaaaag caagatcaat atcattttta gcaataaaat
     5641 cctgagcaga ggcaccgtcg gaggcttcat atacaatatg ttgttcatcc tgaagaatct
     5701 ggcttaagac cttaaggata ttcggctcat cgtcaacaat caagaccttt gccatagaaa
     5761 cccctctatc tattatatat aggaagcgtc aatatgaact ccgctccatt accgggaaca
     5821 ctacgacata ttatttttcc gccgtgctcc ataactattt tttcaacaat tgccagacca
     5881 agaccagttc cttcctgctt tcgggaaaag gttggttcaa aaagtcttcc tagatcttct
     5941 ggaggtatac ccggaccgtt atccctgact cttataatcg ccttgccgtt ttcgatctga
     6001 ctggctatta caatttcacc ataataatcc atagcttcga tagcattttt aataatattg
     6061 attaatacct ggcgaaacag tattctatcg atcctaagat cgggaagctc atcgtcaaga
     6121 aataatttga acacgatatt ttcatgccca tgaaagaaat cgacacaatt ttttaatata
     6181 tcatttatat tttgaaatac aggattaagt tttggtagcc tagtataacg cgaaaactca
     6241 cgcaaaatat gctgtaaggc atccacctct tcgataattg tatcggttcc ggttttgata
     6301 atattctcga tatcatcaga ctgctcgaaa tattttcttc gaatacgttc cgcagaaagc
     6361 cttatcggcg ttaacggatt cttaatctcg tgcataagtt tcgagccgac ttctttccag
     6421 gcctcaagct tttgcatatg catcatggca cgccggctct catcaagctg ctttaccatc
     6481 acattaaaag agttatacaa aaccgaaatc tcgtccgaaa tttcggtttt gtataac
//
LOCUS       DAOTGZ010000160         6534 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_3064
            NODE_14002_length_6534_cov_5.193703, whole genome shotgun sequence.
ACCESSION   DAOTGZ010000160 DAOTGZ010000000
VERSION     DAOTGZ010000160.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868088
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 6534)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6534)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:22:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,785
            CDSs (total)                      :: 2,751
            Genes (coding)                    :: 2,732
            CDSs (with protein)               :: 2,732
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 12 of 19
            Pseudo Genes (internal stop)      :: 5 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6534
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14002_length_6534_cov_5.193703"
                     /isolate="AMR_MDS_3064"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..242
                     /locus_tag="P5123_10050"
     CDS             <1..242
                     /locus_tag="P5123_10050"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRX16647.1"
                     /translation="YVVWIPLSLEDRGSVYQHDIIGQMFDVRTGEEVGQGKYSVNWFE
                     GAYIGWGAARTREEALKQGAGFAVKEITEKMGMNK"
     gene            280..813
                     /locus_tag="P5123_10055"
     CDS             280..813
                     /locus_tag="P5123_10055"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRX16648.1"
                     /translation="MVSSICAMPSATRRRVNVYASGGYIITRDKYLTGNFSDSSEVKM
                     DYSGGPVVLAGLEYNRQNRLSLGLESGHLNISYRNENINMVPVGINSWSDNSFAQGLF
                     VLRIGFNFSYMYVVDHKFISNGLALGPQLSLCYELYDDLYITTNVKYLYFGMNDTREH
                     GHGSTLVPVTVGVIFRL"
     gene            854..1624
                     /locus_tag="P5123_10060"
     CDS             854..1624
                     /locus_tag="P5123_10060"
                     /inference="COORDINATES: protein motif:HMM:NF024845.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zf-TFIIB domain-containing protein"
                     /protein_id="HRX16649.1"
                     /translation="MYKCPNCNNDLIYKNSKIGVTLHCPKCGGIAISTKTAFEVNKRL
                     FTQIWDITANGLGLPGKLCPYCKKEMIQFPLRHDNSDNNFLVDVCPSSQIFWLDNGEL
                     DKAKNYQKDYTIKINSDELRFLANFQKKIVEESPIINQPQKPQIRPLLYGISGSLVGY
                     VVYIFFSSISSIYFYASIGLLLGLIDGIVDGLLDGDYRNIIGKAMLGLCLVPFFSVIY
                     GNIDIFSINDAVEYILIGFSSGLTYSFGRRFVEASFGK"
     gene            1682..2698
                     /locus_tag="P5123_10065"
     CDS             1682..2698
                     /locus_tag="P5123_10065"
                     /inference="COORDINATES: protein motif:HMM:NF024762.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglutaminase family protein"
                     /protein_id="HRX16650.1"
                     /translation="MNSKKITLLITCFNVVFFLNCSKNNENMSIFEYLEQPEEKWNIG
                     VAALLLSKDMYPDIEFSKYTNIIDGIASRVEVYVQNRTDPEYRIAAINTVLFREYGFG
                     YDKNDFLGRNPYNHFISSVLDRRKGVCTSLPLLYMIIAEKLGYPICAVEAPDHTFCRY
                     VLENGNYLNIEVTSGGGEVPDLFYIDDMNIPKQPFKNGVYMRTLSKKEYVGSLLHKNA
                     VSYMIKGDFKTAVNYLEKSVSLHPKNSHNHKVLAHCYGKICNSLESAKERKPYYLKAI
                     EHEKIAEGLGIAPPCPEDYWKIIVKKDTEIVTSTSGNITKINDESLEEKLKVLEVKYH
                     DYFE"
     gene            2682..3464
                     /locus_tag="P5123_10070"
     CDS             2682..3464
                     /locus_tag="P5123_10070"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRX16651.1"
                     /translation="MITLNKIVIVLLILAFPCISYSRYYDPQTSRWISVDPALTTGEY
                     YPTGDKNNDAELPGMGGVFNPVNLNGYNYSGNNPVKFVDPDGRLLAEKNQNGQIVLKV
                     RDWFKSSFTKASIPTLTGYAFTNDGKSRIEVINNQSDDEGYTSNCHGLTFGKGMYWIQ
                     PKEVPKILKGDNYKQRDVAKGDFPQIGDVAIYSKRGSNDVNDEYFHSGTVLGVDKKNN
                     KVQVMETLGQTEGYRKVWKDIGDTKGFMVKYYHTDEKFKLEK"
     gene            3466..3828
                     /locus_tag="P5123_10075"
     CDS             3466..3828
                     /locus_tag="P5123_10075"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRX16652.1"
                     /translation="MKLDRFIIIIVQVILFFFLGCIKSKNESTTITTEIFNQCMQSCV
                     EHNDPRIRDVIEGIFFIWFRNYEGYSKGFMKPAVTANVIRKKLYSSDEEDGFCSKVDE
                     AHRLPILIISNDVNGKKL"
     gene            complement(3911..5116)
                     /locus_tag="P5123_10080"
     CDS             complement(3911..5116)
                     /locus_tag="P5123_10080"
                     /inference="COORDINATES: protein motif:HMM:NF017984.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019104 - DNA N-glycosylase activity
                     [Evidence IEA];
                     GO_process: GO:0036297 - interstrand cross-link repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="crosslink repair DNA glycosylase YcaQ family
                     protein"
                     /protein_id="HRX16653.1"
                     /translation="MNFSINDIRKYLIIYHNLDERVTPSEIVPLLFKKLGCIQYDPLS
                     ITGRNADLVLQSRVQNYRPSILEKLLYSERKLIDGWDKMMSIYPVSDWALFTRMRNHK
                     EREVKGALEWRKSLHVLEKTSDVIEHLKNNGESKPTDINLGNLDPGKWGHRRESTAVL
                     DYLFHAGQASVARKNGTQKTFDLTQNLLPAKILNSNDPFPDDAVYHRWLVLRRISSVG
                     ILWNRNSVLWQNIGVELKDRGYRQQIIDQLVKENKLEQISIEGVSEIFYINVSNVKLL
                     NSAVGTVLKESMRFIAPLDNLIWDRDMIEQIFGFNYRWEVYMPAAKREYGYYVLPIIY
                     KENFVGRIEPVADRKNSTLVIKNLWKEAESKWTKKMEATLNRELKVFAKFLELNNIEP
                     IRFVARNDH"
     gene            5324..6490
                     /locus_tag="P5123_10085"
     CDS             5324..6490
                     /locus_tag="P5123_10085"
                     /inference="COORDINATES: protein motif:HMM:NF025019.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HRX16654.1"
                     /translation="MYISRNIKDQVQLYLKLFPVILLTGPRQTGKTTFLLNQLSKYKY
                     VTLDDPAEAERAVNMPQQFLDSLSVPVIIDEVQYAPGLFHYIKSAVDANKKPGSYVLT
                     GSQQFNIMHNISESLAGRCGVLSMLTLSFDELSSQQDISLTDYIVQGGYPALYSDSIM
                     KAAYWFPSYINTYLERDVRNIININNLRDFNRFIRALALRSGQILSLSDLARDVGIAP
                     NTAKAWLSTVESSGIVKLLEPYFFNKGKRLVKSPKVYFTDTGLLCHLIGINSYSELLV
                     SPLSGAVWETYAFCQIFHAFLKEGKINPPLWFWRDRTGNEVDFVIEKGGRYILFEAKL
                     TEKPSKKECKGFTKFEAYYGEGSVIRRSIIAPVNKSFPLEDGITVDNGRLATID"
BASE COUNT         1998 a         1126 c         1367 g         2043 t
ORIGIN      
        1 tctatgttgt ttggattcct ctttctttgg aggatagagg ttcagtttat caacatgata
       61 ttataggtca aatgtttgat gttagaacag gtgaagaggt tggacaaggt aaatactctg
      121 tcaattggtt tgaaggcgca tatataggat ggggcgcagc ccgtacaagg gaagaggctc
      181 ttaagcaggg agctggcttt gctgtaaaag agataacaga gaaaatggga atgaataagt
      241 agtatgacaa aaataccatt ttgttttatt tgtttaataa tggtttcatc aatctgcgcc
      301 atgccctcag ctaccagacg tcgggttaat gtctatgctt cggggggata tattattaca
      361 agagataaat atttaacagg aaacttcagt gattcatccg aggttaagat ggattacagt
      421 ggcggtccag ttgtgttagc cggtttagaa tataataggc agaatcgttt gtcattgggt
      481 ttggaaagtg gccatcttaa tatatcgtat cgaaatgaaa acatcaacat ggttcctgtt
      541 ggaataaatt cctggagcga taattctttc gctcaaggat tatttgtatt gcgtattggt
      601 tttaacttct catatatgta tgtggtggat cacaaattca ttagcaatgg tcttgcactc
      661 gggcctcagc tatccttatg ctatgaactg tatgatgatc tttatattac aacaaatgtt
      721 aaatacctct actttggtat gaatgacaca cgtgaacatg gtcacggatc gaccttagtt
      781 ccggtgacgg ttggtgttat ttttaggctg taaatgaaat catgttttaa aactctaatt
      841 aaagagagcc tttatgtaca aatgcccaaa ttgtaataac gatttaatat acaaaaattc
      901 aaaaataggc gtaacactcc attgccccaa atgcggtggt atagccattt ctactaaaac
      961 agcttttgaa gtaaacaaac ggctttttac gcaaatctgg gatataactg caaacggatt
     1021 ggggcttccg ggaaaacttt gcccatactg caaaaaagag atgatacagt ttcctttgag
     1081 acatgataat tctgataaca attttttggt cgatgtttgc ccaagctccc agattttctg
     1141 gctagataat ggtgaacttg ataaagcgaa aaattatcag aaagattata ctattaaaat
     1201 aaattctgat gaactaagat ttcttgctaa ttttcaaaaa aaaattgtcg aagagagtcc
     1261 gattataaat cagccgcaaa aaccgcagat taggcctctt ctttacggta tttccggatc
     1321 tctggttgga tatgtcgtat acattttctt cagcagtatt tcttctatat acttttacgc
     1381 gtcaatcggc ctgttactgg ggttgattga tggcatagtc gatgggctgc ttgacggaga
     1441 ttatagaaat attattggaa aggctatgct tggtctctgt ctggtgccgt ttttttctgt
     1501 aatatacggc aatatcgata tttttagtat taacgatgct gtcgaatata ttctgatagg
     1561 tttcagctct gggctaacct attcgtttgg taggcggttt gtagaagcca gctttggcaa
     1621 atagtcgaca accctgaaag tattttccgg cgcttcagtg cgccagttta taggagatga
     1681 aatgaacagt aaaaagatca ctttattgat tacatgcttc aatgttgtat tttttcttaa
     1741 ttgcagtaag aataatgaaa acatgtcaat atttgaatat ttggaacagc cggaagaaaa
     1801 atggaatatc ggcgttgctg cactccttct atcgaaagac atgtatccag atatagaatt
     1861 ttcaaaatac acaaatatta ttgacggcat tgcttcaagg gttgaggttt atgttcaaaa
     1921 tagaacagac ccggagtata gaattgcggc aatcaacacc gtccttttcc gtgaatatgg
     1981 ttttggatat gataaaaatg attttttagg ccgtaatcct tataatcact ttatttcaag
     2041 tgtattggat cgacgcaaag gagtgtgtac ttcattgccc ttgctttata tgattatagc
     2101 tgagaagtta gggtatccga tatgtgcggt agaggctccg gatcatactt tttgcagata
     2161 tgtattagag aatggaaatt atctcaatat tgaagtaaca agcggaggcg gagaagtgcc
     2221 tgatcttttt tacattgatg acatgaatat cccaaaacag ccatttaaaa atggggtata
     2281 tatgagaacc ctgtctaaaa aagaatatgt cggatcactg ttgcataaaa atgctgtatc
     2341 ttatatgatt aagggagact tcaaaacagc tgtcaattat cttgaaaaat ctgtcagtct
     2401 tcacccaaaa aattcacata atcataaggt actggctcat tgttatggta aaatctgcaa
     2461 cagccttgaa tcggcgaaag aacgaaaacc ttattatttg aaggccatcg aacatgagaa
     2521 aatcgcagaa ggtcttggga tagcgcctcc gtgccccgaa gattattgga agattatcgt
     2581 aaaaaaggat actgaaatag tcaccagtac aagtggaaat ataaccaaaa taaatgatga
     2641 gtctttagaa gaaaaattaa aagtgttgga ggtcaaatac catgattact ttgaataaaa
     2701 tagtgatagt tttactgatt cttgcctttc cctgtatctc atatagcaga tattacgatc
     2761 cccagacaag tagatggatt agtgtcgatc cggcactaac aactggggaa tattatccaa
     2821 ccggtgataa aaataatgat gcagaattac ccggtatggg cggagtgttt aatccagtta
     2881 atttgaatgg atataactac tcaggtaata atccggtcaa atttgttgat cctgacggca
     2941 gacttttagc agagaagaat caaaacggac agattgtatt aaaggttcgt gattggttta
     3001 aaagttcttt tacaaaagca agtataccaa cccttaccgg gtatgcattt actaatgatg
     3061 gaaaaagtcg aattgaagtt attaataatc aaagtgatga tgaagggtat acctctaatt
     3121 gtcatggact cacttttggt aagggaatgt actggattca accgaaagaa gtcccaaaaa
     3181 tattaaaagg tgataattac aaacagaggg atgtcgcaaa aggtgatttt ccacaaattg
     3241 gtgatgttgc gatttatagt aaacgtggta gtaatgatgt gaatgatgag tattttcatt
     3301 ccggtacagt attaggggta gataaaaaaa ataataaagt gcaagttatg gaaacccttg
     3361 ggcaaacgga aggatatcga aaggtctgga aagatattgg agatacaaaa ggttttatgg
     3421 taaaatatta ccatacagac gaaaaattta agctggagaa ataacatgaa gcttgacaga
     3481 tttattatta ttattgttca agttatacta tttttttttc ttgggtgcat aaaaagtaag
     3541 aatgagtcaa caacaatcac tacagaaata tttaatcaat gcatgcaatc ttgtgtcgaa
     3601 cacaatgatc caagaataag agatgttatt gaaggcattt ttttcatttg gtttcgtaat
     3661 tatgagggtt atagtaaggg atttatgaaa cctgccgtta ccgcaaatgt tataagaaag
     3721 aaactttaca gcagcgacga agaggatggt ttttgtagca aagttgatga agctcataga
     3781 ttgccaattt taataatatc taatgatgta aacgggaaaa aattgtaaca agaattatac
     3841 gagtttgaac ttagaattaa accctaatta gcctgctagc gtcctgtgcg caggctgatt
     3901 tatattgttt ttagtggtcg tttcgcgcaa caaatcgtat aggctcaata ttatttaact
     3961 ccagaaattt tgcaaaaact ttgagctctc tgtttaaagt tgcttccatc tttttggtcc
     4021 atttggattc agcttctttc cacaagtttt taataaccaa tgtgctgttt ttacggtcgg
     4081 ctacgggttc gatgcggcca acaaaatttt ctttataaat tattgggagc acgtagtagc
     4141 cgtactccct tttcgcagct ggcatataga cttcccagcg ataattaaac ccgaaaattt
     4201 gctctatcat gtcgcgatcc cagataaggt tatcgagtgg cgcaataaaa cgcatgctct
     4261 cttttaaaac ggtgcctaca gccgaattca atagtttaac gttactcaca ttgatataaa
     4321 aaatctcaga taccccttca atacttatct gttctaattt attttcttta accaattgat
     4381 ctattatctg ctgccgatag cctctgtctt taagttctac tccgatattt tgccaaagta
     4441 cactgttgcg gttccacaag atgccaaccg aagagatacg ccgcaagaca agccagcgat
     4501 ggtagactgc atcgtcggga aagggatcat tagagtttag aattttggcg ggcaacagat
     4561 tttgggtaag gtcaaatgtc ttttgtgtgc cgttctttcg cgccacggat gcctgaccgg
     4621 catgaaacag atagtccaga acggctgttg attcgcgccg gtgtccccac ttgcccggat
     4681 caaggttgcc aaggttaata tctgtcggct ttgactcacc gttattctta agatgttcaa
     4741 ttacgtcact ggtcttttcc agaacgtgaa gtgatttgcg ccattccagt gcgcctttga
     4801 cctccctctc tttatgattg cgcatacgag taaacagagc ccaatcgcta acgggataaa
     4861 ttgacatcat cttatcccag ccatctataa gtttgcgctc gctataaagc agtttttcca
     4921 gtatggatgg tctgtagttt tgtaccctcg attgaagtac caggtcggca ttgcggccgg
     4981 ttatgctaag cggatcgtac tgtatgcatc ccaacttctt gaatagtaat ggaacaatct
     5041 ctgatggggt gactctttca tcaaggttgt ggtagataat taggtatttg cggatatcat
     5101 ttatagaaaa gttcattttg atggagagtc cttttgttca cagaaatcag agcacataat
     5161 caactactca tatgtttagg tcaagagcat ttcggatagt aaatggtgat ctgataaata
     5221 tattgacaat tttgcaattg aattgcaaaa ttgtcaatat atgaatgcaa tatagccaag
     5281 attttgtaaa agtcatctgt aaagctggtt gaggacttaa accatgtata tatccagaaa
     5341 tatcaaagat caggtgcagt tatatctaaa actttttcca gtaattctat taactggtcc
     5401 gcgtcagact ggtaaaacga cttttctgct taatcagttg tctaaatata aatatgtaac
     5461 acttgatgac cctgcagaag ccgaacgagc agttaatatg ccgcaacagt ttcttgattc
     5521 gctttctgtc ccagtaatca ttgatgaggt gcaatacgca cctgggcttt ttcattatat
     5581 aaaatcagct gtggatgcta ataaaaaacc tggctcatat gtattaactg gttctcagca
     5641 gtttaatata atgcacaata tttcagaaag tcttgccggt cgttgcggag tgcttagtat
     5701 gcttactctc tcctttgacg agctttcgtc acaacaagat atcagcttga ccgattatat
     5761 tgtccagggt ggataccccg cgttatattc tgatagcata atgaaggctg catattggtt
     5821 tccatcctat ataaatacat atcttgagcg tgatgtgcga aatataatca atatcaacaa
     5881 tcttcgtgat tttaatcgtt ttatcagggc attggctctg cgttccgggc agatattgtc
     5941 gttatctgat ctggcgcggg atgttgggat cgcgcctaat accgcaaaag cctggttaag
     6001 cacagttgag tcatccggca ttgttaagct tttagaacca tattttttta acaagggcaa
     6061 gcgtctggta aaatcgccta aggtctactt cacagacaca gggcttttgt gccatcttat
     6121 cgggatcaac tcttatagtg aacttcttgt atctccactt tcgggtgcag tttgggagac
     6181 ctatgcgttt tgtcagatat ttcatgcttt tttaaaagag ggtaagatta acccgccact
     6241 ctggttttgg cgcgaccgca ccggaaacga agttgatttt gttatcgaaa agggcggacg
     6301 atatattctg tttgaggcaa agcttacaga aaaaccatcg aaaaaagagt gcaagggctt
     6361 tacgaagttt gaagcgtatt atggagaagg gtctgttatc cgaagatcaa ttattgctcc
     6421 ggttaacaaa tcctttccgc tggaagatgg tatcactgta gataatggcc gtctagcaac
     6481 tattgattaa tcagacctca atgataattg ttcatagata cccctcggta gcta
//
LOCUS       DAOTGZ010000161         6512 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_3064
            NODE_14073_length_6512_cov_4.398173, whole genome shotgun sequence.
ACCESSION   DAOTGZ010000161 DAOTGZ010000000
VERSION     DAOTGZ010000161.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868088
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 6512)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6512)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:22:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,785
            CDSs (total)                      :: 2,751
            Genes (coding)                    :: 2,732
            CDSs (with protein)               :: 2,732
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 12 of 19
            Pseudo Genes (internal stop)      :: 5 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6512
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14073_length_6512_cov_4.398173"
                     /isolate="AMR_MDS_3064"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1434)
                     /locus_tag="P5123_10090"
     CDS             complement(<1..1434)
                     /locus_tag="P5123_10090"
                     /inference="COORDINATES: protein motif:HMM:NF012234.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit"
                     /protein_id="HRX16655.1"
                     /translation="MDYSLNAKKIINELAQGEGRRINSSLLEPEHILLAMLKIPDCGA
                     MKIMNRLGINFDVLRRNLDISLSSKAGPIYIGDIPVSPSFNKILKYARDEAIMLKEFK
                     ILTEHLLLGLFKHKTSAGIKNLIDAGINYNIIVSELKEYSMARDNSRLGSRKKDGSKS
                     SLADFTINLNERAQKGLIDGVIGRESEIERVIQILGRKNKNNPVLVGEAGVGKTVIVE
                     GLAKHIVEGTVPEPLLNKIILSLDMTSVVAGTKYRGEFEDRLKKIMAELREQDNIILF
                     IDELHSIIGAGAAEGALDAANILKPVLARGELQCVGATTLREHKKYIEKDPALERRFQ
                     TVLVNEPSVEDTIKIIMGLQDSYEKHHKVNFTPEALKQSVYLSDRYINDRFLPDKAID
                     VIDEAGSRVRLKNCNPPADISELEEKINSLNEQKIAFVQNQEYEKAASLRDNLHILRN
                     ELEQKKQDWEERRNEYRVTVEEEDVVDV"
     gene            complement(1442..2482)
                     /locus_tag="P5123_10095"
     CDS             complement(1442..2482)
                     /locus_tag="P5123_10095"
                     /inference="COORDINATES: protein motif:HMM:NF012443.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRX16656.1"
                     /translation="MFKNLLDDTSFWSAEGQLSDVVLSSRLRLARNHASLPFPVNNDF
                     QGHAVVEDSLLQFVNNSKFSESLKFYKMSDLDEYDRRFLRERNIITENMENDEQTSLV
                     YDTSQQFCIQVNSGDHLRIQKLCSGLEFYDSYGKIVDIDNELNKYVDYAFNDTFGYLS
                     AFVDNAGTGLKASALLHLPVLSTLGTIFEVKKMAEEMGASFEPVSGDQNQNYGSLYLV
                     CNRKSINHTEPEIIEMLDDIVKMVAGLEMESRDDYSNDYKNQLEDRIWRSYGILKYSR
                     SITYVEALEYLSNIRLGVILSIIKDMNLKDVHDLLVKIQTAHLLKLADRPLQSLMDYD
                     IFRAEYISSVLK"
     gene            complement(2482..3012)
                     /locus_tag="P5123_10100"
     CDS             complement(2482..3012)
                     /locus_tag="P5123_10100"
                     /inference="COORDINATES: protein motif:HMM:NF014232.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UvrB/UvrC motif-containing protein"
                     /protein_id="HRX16657.1"
                     /translation="MLCERCENREATVHLTEIIKGVKSEVHLCEECARTIGLNSKLSN
                     FSLAIPDFLSFLNEDTKEYSDQPEVVICKNCGMDIQTAYKTRKVNCPYCYYFFSEKIS
                     ALLGGSDIHHTGRTPENFTDFSVHNAANKPERSSVNDVSILKSRLDQAVENEEYEEAA
                     LLRDKIKDLEQLCRGN"
     gene            3296..4351
                     /gene="hisC"
                     /locus_tag="P5123_10105"
     CDS             3296..4351
                     /gene="hisC"
                     /locus_tag="P5123_10105"
                     /EC_number="2.6.1.9"
                     /inference="COORDINATES: protein motif:HMM:TIGR01141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004400 - histidinol-phosphate
                     transaminase activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol-phosphate transaminase"
                     /protein_id="HRX16658.1"
                     /translation="MKYWNSTLKSMTEYIPGEQPEDIDEYIKLNTNENAFPPSADILN
                     AITKECSGNLRLYPNPDAETLTKICADTWGLSQSNYFAANGSDELFSLIFRGFIDRDQ
                     IAAFSYPSYSLYYTMSEANGIAYDKIDLKEDFSIDFAKFLKKKYALVIFCNPNNPTGT
                     GVNREGLIKFLEKYKGVAVVDEAYVDFSGNSMIDLVNSFDNLIVTRSFSKSYSLAGLR
                     VGIAAAHRDIIEGFKKIKDSYNVDRLAQAGAIAALKDKKSFNYRVSMINNNKDYLTES
                     LDELGFKTVPSEANFIFTNHSSIDAKTLYEKLKENKILVRHFDGPRLSDYLRITVGSM
                     IEIKAFLKVLRSIIDEI"
     gene            complement(4348..4614)
                     /locus_tag="P5123_10110"
     CDS             complement(4348..4614)
                     /locus_tag="P5123_10110"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRX16659.1"
                     /translation="MKRKYYALSVVIIFVLLVLYYIGTREICYQVCADVCGERECVNQ
                     CTPLYKIVSRTCTINPAEGKVSDEAALGALIRAHGLSAIKKLFR"
     gene            4676..5473
                     /locus_tag="P5123_10115"
     CDS             4676..5473
                     /locus_tag="P5123_10115"
                     /inference="COORDINATES: protein motif:HMM:TIGR01856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004401 - histidinol-phosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol-phosphatase HisJ family protein"
                     /protein_id="HRX16660.1"
                     /translation="MNKYDYHIHGTFSSDSTAAYGDLIQKAINENYQQIAFTDHFDFL
                     PSEALEYGVPSYTEYVKHIENLQTKFKSSMSILLGVEIGEFHRVTSYTDRLFKIRKPD
                     LTIGSIHVLTDGFNVSVPFNEKMTKKQVLDYYNENLALCEQSPINILGHLGIHKRYYT
                     QTPDESYVDSLIDKILETLIKREIALEINYSPFRKTYNSFLPEIPILKRYLTKGGKLV
                     TIGSDSHSVENFDDNYNRTAKILFDCGCKELAIISPNGWATTPLSCF"
     gene            complement(5479..>6512)
                     /locus_tag="P5123_10120"
     CDS             complement(5479..>6512)
                     /locus_tag="P5123_10120"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF021127.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042578 - phosphoric ester hydrolase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0045227 - capsule polysaccharide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="CapA family protein"
                     /protein_id="HRX16661.1"
                     /translation="FAGDIMMHIRVKKCAAINGFDHLFKRISPELESVDYMSGNMEFP
                     VSPPFKSRGIIFNAPEASIPAVKKAGFDLVVLANNHILDQKEKGLLDTIGFLEKYNLP
                     FIGVKKTEKDARLGHVEKINGIRVGMTAYAGLINYPDRNRGSNFYVNWIYKKRNVLED
                     IKEMKKRCDFLVMQVHTGVEYVLEPRQSDRELFQEYLDAGVDLIIGHHPHMLQSMETY
                     KTKDGRTTEIFYSLGNFVHDQTRSVAIRKTKKSISIKSSAIVKLHLTRVGSSIVDRYT
                     VVPIHTVHEFLESGGQKYKNIQTATLKDLVKESEGNAGLSKYYKNETEAVRLTLFKGK
                     IPSNITFIE"
BASE COUNT         1992 a         1357 c         1072 g         2091 t
ORIGIN      
        1 aacatcgaca acatcttctt cttctaccgt tacgcgatat tcgtttcttc gctcttccca
       61 gtcctgtttt ttttgttcaa gctcatttct caatatatgc aaattgtccc ttaaagaggc
      121 cgctttttcg tactcctggt tctgaacaaa ggctattttt tgctcgttaa gtgaatttat
      181 tttttcttcg agttcggata tatcagcagg cggattacag tttttcaatc ttactcttga
      241 accggcctca tcaattacat ctatggcctt gtctggcaaa aaccgatcgt taatatagcg
      301 atctgaaagg taaaccgact gttttaatgc ttcaggtgta aaattcacct tatgatgttt
      361 ttcgtaacta tcctgcagac ccataattat ttttatggta tcctcaactg aaggttcatt
      421 aaccaaaacc gtctgaaaac ggcgttcaag agcaggatct ttttcgatat atttcttatg
      481 ctcacgaaga gttgtagcac ctacgcattg aagctcgcca cgggcaagaa caggttttaa
      541 tatattcgcg gcatcaagtg caccctctgc tgctcctgct ccaataattg aatggagttc
      601 gtcaataaaa agaataatat tatcctgttc acgaagctct gccattattt tcttaagacg
      661 atcttcaaat tcaccgcgat atttggtacc agcaacaaca gaagtcatat caagcgacaa
      721 tattattttg tttaaaagcg gctcgggtac agtgccctca acaatatgtt ttgccaaacc
      781 ttcaacaatt actgtttttc caacacctgc ttcacctact agcacagggt tattcttatt
      841 tttacgcccc aatatctgta ttacacgttc aatctcggac tctcttccaa taactccgtc
      901 gatcaaccct ttttgagcac gctcattaag attaatcgta aaatcagcaa gcgagctttt
      961 agaaccgtct ttttttcggc ttccaagacg cgaattatca cgcgccatgg aatactcctt
     1021 aagctcgcta acaataatat tataattgat acccgcatca ataaggttct ttattccggc
     1081 actggtttta tgcttaaaaa gtccaagtaa cagatgttct gttaagattt taaactcttt
     1141 gagcattatt gcctcatctc tggcatattt cagtatttta ttaaaagagg gagatacggg
     1201 gatatcacca atatagatgg gacctgcctt ggaagataac gatatatcaa gatttcgtct
     1261 caacacatca aagttgatac caaggcgatt catgattttc atggcaccac aatctggaat
     1321 tttaagcatg gccaacaata tatgttcggg ctccaagaga gaagagttga ttcgtctacc
     1381 ctcaccctgt gccaactcat ttattatttt tttcgcattt aacgaataat ccataagcac
     1441 ctcatttaag caccgagctg atatattccg cacgaaatat atcataatcc attaacgact
     1501 gcaaaggtct gtccgcaagc ttaagcaaat gcgcagtttg tatttttaca agaaggtcat
     1561 gaacatcttt cagattcata tctttaataa tactaagaat aacacctaac ctaatattcg
     1621 aaaggtattc aagagcttct acataggtaa tcgaacgtga atatttaagt ataccataag
     1681 aacgccatat gcggtcttca agctgatttt tataatcatt gctgtaatcg tcccttgact
     1741 ccatttcaag accggcaacc atttttacta tatcatcaag catctcgatt atttcaggtt
     1801 cggtatggtt tatagattta cgattacaaa caagataaag tgatccatag ttctgattct
     1861 gatctccact aacaggctca aacgacgcac ccatctcttc ggccattttt tttacttcaa
     1921 aaatagtacc cagagttgat aatactggca aatgcaaaag tgccgacgct ttcagtccag
     1981 tgccagcatt atctacaaaa gcagaaagat aaccaaaagt atcattgaag gcataatcta
     2041 catacttgtt cagttcattg tcgatatcga ctattttacc atatgagtca taaaactcga
     2101 gcccggaaca taatttttgg attcgtaaat gatcaccgct atttacctgt atgcaaaact
     2161 gctgtgacgt atcataaaca agagaggtct gttcatcatt ttccatgttt tcggtaataa
     2221 tgttccgctc tcgtaaaaat cttctatcgt attcatcaag atcactcatt ttgtaaaatt
     2281 tcaatgactc gctgaatttt gaattattta cgaactgtag caaagaatcc tctacaacgg
     2341 catgtccctg aaaatcattg tttacgggga aaggcaaact tgcatggtta cgagcaaggc
     2401 gcaaacgaga ggagagcaca acatcagata gctgcccctc ggcagaccag aaagaggtat
     2461 catcaagtag atttttaaac atcagttacc ccggcagagt tgttccagat cttttatttt
     2521 gtcacgaagg agagccgctt cttcatactc ttcattttcg actgcttggt ctaaacggct
     2581 ttttagaatg gaaacatcgt ttacagacga tctttcaggc ttattcgctg cattatgaac
     2641 cgaaaaatcg gtaaaatttt caggagttct tcctgtatga tgtatatccg aaccacctaa
     2701 taaagcagaa atcttttccg aaaagaagta ataacagtaa ggacagttaa cctttcgggt
     2761 tttgtaagcg gtttgaatat ccattccgca gttcttacat attacaactt ctggctgatc
     2821 actatactct tttgtatctt cgtttaaaaa agataaaaaa tcaggtatcg ctagtgaaaa
     2881 attagatagt ttagaattta gaccaatagt tctcgcgcac tcttcacaaa gatgcacctc
     2941 ggacttaacc cctttaataa tctcggtcaa atgaactgtt gcttctcgat tttcgcatct
     3001 ctcacataac atagcgttca ctcttattat aaagtacctt gatatatatt accgcaatta
     3061 ctgttttatg tctacaaaaa ttttttacag cgttatatta tgtagctctt gactgtttta
     3121 tctttttatc aatgtaaccg attgcattga tttggttgta attaatttaa agttcttttg
     3181 tttttttact ttttacattt ttttcatttt tgattgcaaa ttaaacacaa aagaatattc
     3241 aggacattgt tacattatgt aaacaatacc tttcgtttat tgcaccgagg ttatcgtgaa
     3301 atattggaat agtacactta aatccatgac agaatatatt cccggagaac agcccgaaga
     3361 cattgatgaa tatattaaac ttaatacaaa tgaaaacgcg tttcctccat cagcggatat
     3421 tttaaacgct attacaaaag agtgcagcgg aaaccttcgc ctatacccta atcctgatgc
     3481 agaaactctt acaaagatat gtgccgacac ctggggactt tctcaatcga attatttcgc
     3541 ggcaaacggc tctgatgagc tcttttctct tatttttcga ggttttatag accgggatca
     3601 aattgctgct ttctcgtacc cttcgtactc tctatactat acaatgagcg aagcaaacgg
     3661 tatagcctat gacaagattg atctaaaaga agacttcagt attgactttg ccaaatttct
     3721 aaagaagaaa tacgcgcttg taatattctg taaccccaac aaccctactg gtacaggcgt
     3781 taacagggaa ggtctaataa aatttttgga aaagtataaa ggtgtcgcag tagtcgatga
     3841 agcctatgtg gatttctctg gaaacagcat gattgacctg gtaaactctt ttgataatct
     3901 gattgtaact cgttcttttt caaaatctta ttctcttgcc gggctaagag ttggaatagc
     3961 cgcggcacac agagacatta ttgaggggtt caaaaaaata aaagattcat acaacgttga
     4021 caggttagcg caagctggag caatcgccgc cttaaaggac aagaagagct ttaactaccg
     4081 cgtatcaatg atcaataaca acaaggatta tctaacagaa agtcttgatg aactaggttt
     4141 taaaactgtt ccttcagaag caaactttat ctttaccaac cacagctcta ttgacgcaaa
     4201 gactctctat gagaaactaa aagaaaataa aattcttgtt cgtcattttg acggtccacg
     4261 tttatcggac tacttgcgaa ttactgtagg ttccatgatc gaaataaaag cttttttaaa
     4321 ggtattgcgc tcaattattg atgaaatcta acgaaaaagt ttttttatag cggataatcc
     4381 atgcgcgcgt atcaaggctc ccaatgccgc ttcatcactt accttgcctt ccgcggggtt
     4441 tatggtacaa gttctggaga caattttgta aagaggtgtg cattgattta cacattctct
     4501 ttctccgcat acatcggcac aaacctgata acatatttcg cgtgtaccta tataatataa
     4561 aacaagcaaa acaaatatta taacaacact taatgcataa tattttcttt tcataattaa
     4621 ccagttaata agtattttat attgacacat tattttttta ggataaatta accgcatgaa
     4681 taaatacgat tatcatatac atggaacctt tagcagcgat agcacagctg cctatggcga
     4741 ccttatacaa aaagcaatta acgaaaacta ccaacagatc gcttttaccg atcatttcga
     4801 ttttcttcca tctgaggcac ttgaatatgg tgtcccatct tatacagaat atgttaaaca
     4861 tattgaaaat ttacagacaa aattcaaatc gtctatgtca attttactcg gagtcgaaat
     4921 aggagagttt caccgagtaa cttcttatac cgacagatta ttcaaaattc gcaaaccaga
     4981 tttgacaata ggatcaatac atgttttgac tgacggtttt aatgtctctg tacctttcaa
     5041 cgagaaaatg acaaaaaaac aggtgcttga ctattacaac gagaacttgg ctttgtgcga
     5101 acaatcacca attaatattc ttggacatct tggcatccac aagcgttact acacgcaaac
     5161 tcccgatgag tcttacgttg attctttaat tgataaaata ttagagacct tgataaagcg
     5221 cgaaatcgcg cttgaaatta actattctcc atttagaaaa acctataaca gttttttacc
     5281 ggaaattcct attttgaaac gttacctaac taaaggaggc aaacttgtca caatcggttc
     5341 agactcacat tctgttgaaa attttgatga caactataac cgtaccgcaa agattctttt
     5401 tgactgcgga tgcaaagaac ttgccattat ttcacccaac ggctgggcaa ccacaccact
     5461 aagctgtttt tgataatgtt attctataaa tgttatgtta gacggtattt tacccttaaa
     5521 tagtgttaat ctgacggcct cggtttcgtt tttataatat ttactcagtc cggcattgcc
     5581 ctcactctct ttcacaagat ctttcaatgt cgctgtctgt atattcttgt acttttgccc
     5641 accactctca agaaactcat gaacggtatg tataggcaca actgtataac ggtcaacaat
     5701 cgaactacct acacgagtaa gatgtaattt tacaatagca gagcttttta tacttatact
     5761 tttcttggtt tttcgtatgg ctactgagcg agtttgatca tgaacaaagt taccaagact
     5821 gtaaaatatt tcggttgttc tgccgtcttt ggttttatag gtctccattg attgaagcat
     5881 atgagggtga tgcccgatta ttagatccac cccagcatcc agatactcct gaaaaagttc
     5941 tctgtcagac tgacggggtt ctagtacata ttcaactcct gtgtgaacct gcataacaag
     6001 aaaatcacat ctttttttca tttcttttat atcttctaac acatttctct tcttgtatat
     6061 ccagtttaca taaaaattac tgcctctatt cctgtccgga tagtttatca gaccggcata
     6121 ggccgtcatg cctaccctta tcccatttat tttttcgaca tgaccaagac gcgcatcttt
     6181 ttctgttttt tttacaccta taaatggcaa attatatttt tccagaaaac caatagtatc
     6241 gagtagaccc ttctctttct gatcaagaat atggttatta gccaatacta caaggtcaaa
     6301 acccgctttt ttaacagcag gtattgaggc ctcaggcgca ttaaatataa ttccccttga
     6361 cttaaatggt ggcgaaaccg gaaattccat attgccgctc atataatcga cactctcaag
     6421 ttcgggcgat attcttttaa acagatgatc aaaaccgttg atagccgcac atttctttac
     6481 acgaatatgc atcattatat cgcccgcgaa ac
//
LOCUS       DAOTGZ010000162         6416 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_3064
            NODE_14343_length_6416_cov_4.614369, whole genome shotgun sequence.
ACCESSION   DAOTGZ010000162 DAOTGZ010000000
VERSION     DAOTGZ010000162.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868088
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 6416)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6416)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:22:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,785
            CDSs (total)                      :: 2,751
            Genes (coding)                    :: 2,732
            CDSs (with protein)               :: 2,732
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 12 of 19
            Pseudo Genes (internal stop)      :: 5 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6416
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14343_length_6416_cov_4.614369"
                     /isolate="AMR_MDS_3064"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..2201
                     /locus_tag="P5123_10125"
     CDS             <1..2201
                     /locus_tag="P5123_10125"
                     /inference="COORDINATES: protein motif:HMM:NF012742.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="substrate-binding domain-containing protein"
                     /protein_id="HRX16662.1"
                     /translation="LISYVGDTIDFEDATVMNNNKLYEYISNKRIDGAIVLISSVSSH
                     VNKAGKIEFMRKIAERCPVVSVGDILEAYPSIGVDNHNGMFDAVNHLITRHGKRHIGF
                     VGGPGNSDEASMRFDAYKAALKQNNIAYDERLYYEGNFVFKSGVEAIDAFTSRKVSFD
                     AIVAASDWMAFGAIQQLKQLGYKIPEQIAVCGFDNVESAYVSTPPLSSVKQPVFEMGQ
                     KAITTIVDIIDKKPFDKKTVYPTEFIIRDSCGCLMSKTETENDDENNVKNSNTSENSA
                     RKNLKAQLIEKLHQDLAEGDGHEFLHFFNTAVDNVKRTEDITDYQKLIFEIREDLLKP
                     TAVKAANENQLEEFKKQKKIKHEAVELFLDKISSYTYKIDEYFHRARLIVSRKAEQLQ
                     FVKYLDANNKSSQIISIGQNILTTYSRDYLFSIIKRDFPKVDIQSCSIVEYCEPDAAP
                     KSENSKILYYYNKDRETSSTDEQFNTDLLVPDKYWPEESRSPWIANAVPMSTQDGCVG
                     YVLFESKNRFGLIYQSFASEIVSALKGIKIYEDHEKVSDSIQQRSRRINDLVTPMLES
                     ILQVANISGDEIQAIRNLSDMTAAGLSQLKSANDIIAKAAASINQMLNMIDMIEDVSA
                     NINILALNASIESARAGTYGLGFQVIAEEIKHLSDTTATNTKNMSEVLRSVVSNIEES
                     SSASQENYQVFAKVQDHVEQVTKLLTNVTNKMNELSSASNDILSEIERKI"
     gene            complement(2322..3299)
                     /locus_tag="P5123_10130"
     CDS             complement(2322..3299)
                     /locus_tag="P5123_10130"
                     /inference="COORDINATES: protein motif:HMM:NF013045.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA];
                     GO_process: GO:0009451 - RNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pseudouridine synthase"
                     /protein_id="HRX16663.1"
                     /translation="MRLLECVVDISQDEERLDSYLALRFDYLSRNRWQKLITDEKLTL
                     NGKIVRRPATRIKKGDTIVFEGNIRQEPDVDSNIDIVYQDDQLLVVNKSGDLPVHPAG
                     RYFNNTALRIMESRLNRKLYPVNRIDRETSGILIFALNSSVACLLAEEWNNCHKSYIA
                     LVRGEFIEDRSCNMPLGLANPSCCTDVDKVLKKRAAYEGAPEKAFTSFSRITGTKDFS
                     LVRAVLGTGRQHQIRAHLEYMGYPLVGDKIYGGDERIYLDFIKNGITNQTIEKVGHRR
                     CMLHAYKAEIVTKKGKTAFIVEPPDDFILCAKNLAGIDCMVDIKKILNS"
     gene            complement(3304..3909)
                     /locus_tag="P5123_10135"
     CDS             complement(3304..3909)
                     /locus_tag="P5123_10135"
                     /inference="COORDINATES: protein motif:HMM:NF020893.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DedA family protein"
                     /protein_id="HRX16664.1"
                     /translation="MFFDTLPPFIIYIILFFAGVLENLIPPLPGDTVMLFGAYLSSQG
                     VLSFDFAFISTTAGSVAGYIALFAVAYKYGVQTIRRFGARFFSEKSLSRAEEYLRRYG
                     YLVILINRFLPGLRSVISIVTGYSRCRFLPVLLLSMISASVWNFIWMYTVYKLGGNWE
                     AVKTECLRIIALYNRAAIFVLIVLIVVFFVVWLLKKWIKKA"
     gene            complement(3927..4391)
                     /locus_tag="P5123_10140"
     CDS             complement(3927..4391)
                     /locus_tag="P5123_10140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007231304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HRX16665.1"
                     /translation="MAIQENSVVSMEYCLTDNQGNVIDTTKDGSLFSYIHGSGMIIKG
                     LENELTGKNPGDIFKVAISPENAYGPVYDELIQQVPIEQFNGIENLQKGMQLQAQSSE
                     GNYLVTVAEVGEKEVTLDGNHPLAGVELNFDVEVVAVREATPEELDSGQVNG"
     gene            4878..5720
                     /locus_tag="P5123_10145"
     CDS             4878..5720
                     /locus_tag="P5123_10145"
                     /inference="COORDINATES: protein motif:HMM:NF024376.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate/phosphite/phosphonate ABC transporter
                     substrate-binding protein"
                     /protein_id="HRX16666.1"
                     /translation="MKAFYCVLLTVLNLLFINTLSAQTRISMGISPYTTPLTSNTKQD
                     KFIRYIEEKTDTSITPVVFKNDEELLQYFRQGKIDFAWLGTFQYMKYKTELYSYVIAR
                     PIRQNRDYYRGAFITHSSNEISGFNDLRSKSIAFVSHTSQAGYIFPVLSLYTSGLTLG
                     KDYYPEFLRGHDNVIYAVQQKRYDAGVVFDSALEVYLNDTQRSQIKIIGYTRDIPFEP
                     FIISKRIDKELVETIRNAIIECSSPVLLKDLKVDRFVPDTDTAYKIYNPSSFIIINEV
                     KETP"
     gene            complement(5751..>6416)
                     /locus_tag="P5123_10150"
     CDS             complement(5751..>6416)
                     /locus_tag="P5123_10150"
                     /EC_number="2.7.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013740379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6-phosphofructokinase"
                     /protein_id="HRX16667.1"
                     /translation="TAFSEAVKVVQRAHVEAKGVPNGIGLVKLMGRQSGFIAAHATLA
                     CNDVNFALIPEVPFDLEGEKGFLKILEDRLERSDHAVIVVAEGAGQDLIQSESHGESS
                     FDASGNIKFNDIGLFLKQKIEHYFHSKSKEVNLKYIDPSYIVRSAPANENDSLFCAVL
                     GQYAVHAAMAGKTDMIVGQWNGMITHIPIELAVSKRKQIDPDSRLWYDVLAATGQPLS
                     MKN"
BASE COUNT         2093 a         1402 c         1091 g         1830 t
ORIGIN      
        1 accttatatc ctatgtggga gatacgatag acttcgaaga cgcaaccgtc atgaataaca
       61 acaaattata tgaatatata tccaataaac gtatcgatgg tgctatagta ttgatcagct
      121 cagtatcttc tcatgttaac aaagcaggca aaatagagtt tatgcgcaaa attgccgaaa
      181 gatgccccgt tgtaagtgtg ggcgatattc tagaggcata tccttctatc ggtgttgata
      241 accacaatgg aatgtttgac gcggtaaatc accttatcac ccgccacggc aaacgccata
      301 ttggatttgt cggtggtcct ggcaacagcg atgaagcttc tatgcgattt gacgcatata
      361 aggcagctct gaaacagaac aatatcgctt acgacgagcg gctctactac gagggcaatt
      421 ttgtcttcaa atcaggagtc gaagccattg acgcctttac ttccagaaaa gtcagctttg
      481 acgctattgt tgcagcaagt gactggatgg cttttggtgc aatacagcag ctcaagcaac
      541 ttggttataa aatccccgag cagatagctg tctgcggttt tgacaacgtc gagtcggctt
      601 acgtatcaac tccacctctt tcatctgtaa agcagcctgt atttgaaatg gggcaaaaag
      661 ctattacaac aattgtagac attatagata aaaagccttt tgataaaaaa actgtctatc
      721 ctacagaatt tattatacgt gactcttgcg gctgccttat gtccaagacc gaaacagaaa
      781 atgatgacga aaacaatgta aagaactcga atacaagcga aaactccgct cgaaaaaatc
      841 ttaaggctca gcttatagaa aagctgcatc aggatcttgc cgaaggtgac gggcatgaat
      901 ttcttcattt ctttaacacg gctgtagaca atgttaagcg caccgaagac attactgatt
      961 atcaaaaact aatcttcgaa ataagagaag atttgctaaa gccaaccgct gtaaaagccg
     1021 ctaatgagaa ccaacttgaa gaatttaaaa aacagaaaaa aatcaagcat gaagccgtag
     1081 agctatttct ggataaaatt agttcttata catataaaat tgacgaatat ttccatcgtg
     1141 caaggcttat tgtttcgcgc aaagccgaac aattgcagtt tgttaagtat ctggatgcca
     1201 acaataagtc atctcagata atctcaattg gacaaaacat tctaacaacc tacagtcgag
     1261 attatctctt ttcgattatc aaaagggatt ttcccaaggt agacattcaa agttgctcca
     1321 tagtggaata ctgcgagcct gacgccgcac caaaatcaga aaactcaaag atactatatt
     1381 attacaacaa ggacagggaa acatcttcca cagacgaaca gttcaatact gatcttcttg
     1441 tccccgataa atactggcca gaagagtccc gttcaccttg gatagcaaat gctgtaccga
     1501 tgagcacaca agacggttgc gtgggatatg ttctctttga atccaaaaac agatttggtc
     1561 taatatacca atccttcgct tcggaaatag taagcgcact taaaggaata aagatttacg
     1621 aagatcacga aaaggtttcc gattcaattc aacaacgaag ccgacgtatt aacgatcttg
     1681 ttactcccat gcttgaatcc attctgcagg ttgccaatat ttctggtgat gagattcagg
     1741 ccatacgtaa tctttctgat atgactgcag ctggactttc ccaattaaaa agtgccaacg
     1801 acataatcgc aaaggcagct gcgagcatta accaaatgct taacatgata gacatgattg
     1861 aagatgtctc ggcaaacatc aatattcttg cgcttaacgc ttcaatagaa tcggctcgtg
     1921 cgggtacata cggactgggc tttcaggtaa tcgcggaaga gataaaacac ctttcagata
     1981 caactgcaac aaatacaaaa aacatgtcag aagttttgcg aagtgttgta agtaatatcg
     2041 aagaatcaag ttcggctagc caggaaaact atcaggtatt tgccaaggta caagatcatg
     2101 tcgagcaggt cacaaaacta cttacaaatg taacaaacaa gatgaacgag ctttcttcag
     2161 caagtaatga tattctttct gaaattgaaa gaaaaatata atagaacttc taaaaataca
     2221 atgtttcaga gggttacata gtttaattat ctattataaa gaaatttgac acaagttttt
     2281 tgaggaaaag taaattttta gaacttaaca tagtgtagtc tttatgaatt gagtattttt
     2341 tttatgtcca ccatgcaatc tataccggca agattttttg cacacagtat aaaatcatcg
     2401 ggaggttcaa caataaaggc agttttccct ttttttgtaa ctatttcagc tttataagca
     2461 tgtagcatac atcttcgatg cccaactttt tctattgtct gattggtaat tccgtttttt
     2521 ataaagtcga gataaattct ctcatcacca ccgtatatct tatcaccaac aagcggatat
     2581 cccatatact caagatgcgc ccttatctgg tgttgccgtc ccgttcccag tacggccctt
     2641 acaagggaaa aatcctttgt acctgtaatc ctgctaaaag acgtaaaagc tttttccgga
     2701 gccccttcat aggctgccct ttttttgaga actttatcta catctgtaca acaacttgga
     2761 ttagccagcc ctaaaggcat gttacacgat ctatcctcta taaactcacc cctgacaagg
     2821 gcgatataac ttttatgaca attgttccat tcctcagcca ataagcaggc aaccgaagaa
     2881 ttaagcgcaa atatcaatat ccccgatgtt tcacggtcta tccggtttac aggatagagc
     2941 tttctgttta atcttgactc cattattctt agagccgtat tattgaaata tcgtccagca
     3001 ggatgtaccg gtaagtcacc tgacttattt acaaccaaaa gctgatcatc ctgatataca
     3061 atatcaatat ttgaatctac atcgggttct tgacggatat tgccctcaaa tactatcgta
     3121 tccccctttt ttattcgagt agcaggtcgt ctaacaattt taccattcaa ggtcagcttc
     3181 tcgtctgtta tcaacttttg ccatcggttt cgtgacaaat aatcaaaacg taatgctaaa
     3241 taactatcca gccgttcctc atcttgagaa atatctacca cgcattcaag caaacgcaaa
     3301 acattaggcc ttttttatcc atttttttaa taaccaaact acaaaaaaca ctacgatcaa
     3361 cactataagc acaaaaatag ccgctctgtt atagagggca attatgcgaa gacattctgt
     3421 tttaacagcc tcccaattac cgccaagctt atacacggtg tacatccaga taaaattcca
     3481 aacagaagca cttatcatgg acaacaataa aaccggcaaa aatcggcaac ggctataccc
     3541 tgtcacaata gatataacag agcgtaatcc tggcaaaaac ctgttaatca gaataaccaa
     3601 atacccgtat cgacgcagat actcttcagc acgcgataaa gatttttccg aaaaaaaacg
     3661 ggctccaaac ctccgaatgg tctgaacccc gtacttatat gctaccgcaa aaagagcgat
     3721 ataaccagca acacttcctg ccgtagttga aatgaatgca aaatcaaatg ataaaacacc
     3781 ctgagaagat aagtatgctc caaaaagcat cacagtatca cctggtaatg gaggtatcaa
     3841 attttccaat actcccgcaa aaaaaagaat aatatagatg ataaacggcg gcagggtatc
     3901 aaaaaacata atggcctcta caacaactat ccattcacct gaccactatc aagctcttca
     3961 ggagtagctt cacgaactgc gaccacttct acatcaaaat taagctcaac tcccgcaagt
     4021 ggatggtttc cgtcaagagt tacctctttt tcaccaactt ccgccactgt aacaagataa
     4081 ttaccttcac ttgactgagc ctgcaattgc atgccttttt gaagattttc aataccatta
     4141 aactgttcaa ttggcacctg ctgtattaac tcatcatata caggtccgta tgcattttca
     4201 gggctaattg ccactttaaa tatatcaccc ggatttttac cagtcagttc gttttccaat
     4261 cctttaatga tcatacctga tccatgaata taagaaaaaa gtgaaccatc ttttgttgta
     4321 tcaataacat tcccctgatt gtcggttaaa caatattcca tagaaactac tgagttttcc
     4381 tgaattgcca taataatctc ctgattgttt ttttccactc tgacaataat ttaccataat
     4441 ttgttataga acccactaaa aatcccaaaa atcgttcata tcaaccttca tcaaggtttc
     4501 tctttgaaaa acagtaattc ttctaagttc ctttattata aaatcattgc aaaatggttt
     4561 cattagaaca tacttatgtt ctaagtatat tctatatacc atacttcaaa aactacctgt
     4621 atgtcaaggt tattgatttt atttttttaa tttgaataac atgtcaatag ggaagtatta
     4681 tccattattt tcaagctgca ccaattactg gctttttctg atgtttcaca agccaaagtt
     4741 caacatatca gtttctttct tgacacaaac cctagttcta atgaataaaa ttttatcaag
     4801 atattaaaga aatttaacac cgatttttac aacacataca tataattaac attacgaata
     4861 acatctggag acaagtaatg aaggccttct attgtgtttt actgactgtt cttaatctac
     4921 tgtttataaa cacactttct gcgcaaacgc ggatatctat gggaatatca ccttacacta
     4981 ctccgctaac atcaaacaca aaacaggata aatttattag atacattgaa gaaaaaacag
     5041 atacctcaat aactccggtt gtcttcaaaa atgatgaaga acttctgcaa tactttcgac
     5101 aaggtaaaat cgattttgcc tggctgggaa ctttccaata catgaagtat aaaacagagc
     5161 tctactctta cgttattgct cgtcccatac gacagaacag agactattac cgaggagctt
     5221 ttattacaca ttcttcaaac gaaatcagcg gatttaacga cttacgtagc aaatcaatcg
     5281 cttttgtctc tcacacatct caagctgggt atatttttcc agtactctct ctatacactt
     5341 ctggcttaac actcggcaag gactattacc ctgagttttt acgcggtcac gacaatgtaa
     5401 tttacgctgt acaacaaaaa agatacgatg cgggagttgt atttgactcg gctcttgaag
     5461 tatatctgaa cgacacacaa agatcccaga ttaagataat cggatacaca agagatattc
     5521 cctttgaacc gtttataatt tccaagcgca tagacaaaga gcttgtcgag accattagaa
     5581 acgcaattat cgaatgcagc tcgccagtac tgttaaaaga cctgaaagtt gacaggtttg
     5641 ttccggatac cgacacagcg tataagattt ataacccgtc atcttttata ataatcaatg
     5701 aagtcaaaga gactccctga aacagcattg aaatagaaaa agcctggttt ctagtttttc
     5761 atggataagg gctgaccagt cgcggccagc acatcatacc agagcctcga atcaggatcg
     5821 atctgcttcc ttttggaaac agcaagctcg atcggtatat gcgtaatcat tccgttccac
     5881 tgtccaacaa tcatatccgt tttccctgcc atcgcggcat gcacggcata ctgtcccagt
     5941 accgcacaaa aaagtgagtc gttttcgttg gccggggcac tcctgacaat atagcttgga
     6001 tctatatact taaggtttac ctctttggat tttgaatgga agtaatgctc aatcttctgt
     6061 ttcagaaaca atccgatgtc gttaaacttt atatttcctg aagcatcaaa actggactca
     6121 ccgtgagatt cggactgaat cagatcctga ccggcgccct ctgccacaac aataacagcg
     6181 tgatcacttc tttcaagacg atcttcaagt atttttaaaa atcctttttc accttcaagg
     6241 tcaaacggga cctcaggtat cagcgcgaaa tttacatcat tgcaagccaa tgtagcgtgt
     6301 gctgcaataa acccggattg tctgcccatc agcttaacaa gaccaatgcc gttaggaact
     6361 ccctttgcct cgacatgcgc acgctgaaca acctttacag cttccgaaaa agctgt
//
LOCUS       DAOTGZ010000163         6405 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_3064
            NODE_14368_length_6405_cov_4.770551, whole genome shotgun sequence.
ACCESSION   DAOTGZ010000163 DAOTGZ010000000
VERSION     DAOTGZ010000163.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868088
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 6405)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6405)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:22:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,785
            CDSs (total)                      :: 2,751
            Genes (coding)                    :: 2,732
            CDSs (with protein)               :: 2,732
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 12 of 19
            Pseudo Genes (internal stop)      :: 5 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6405
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14368_length_6405_cov_4.770551"
                     /isolate="AMR_MDS_3064"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1170
                     /locus_tag="P5123_10155"
     CDS             <1..1170
                     /locus_tag="P5123_10155"
                     /inference="COORDINATES: protein motif:HMM:NF025112.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HRX16668.1"
                     /translation="HGTVTVFIDRIPFEITTYRCDGKYVDGRRPETISFSETLEEDIV
                     RRDFTINGLAYDMRDGTIIDYVEGLKDIKRGLITTIGDPLERLSEDGLRSYRACRFAA
                     KLNFQISPETLTGITQTLEISAKVSVERIRDEIMKMMGTKTPSIGIEYMRETGLLKQA
                     LPELDACYGVEQNRFHKYDIYYHCVYSCDAASPGDPLIRFASLMHDVGKVPCRGVGAD
                     GDFTFYNHEIVGARMIRALMRRLKFSNDDINRVVNLVSNHMFYYTDEWTDGAVRRFIR
                     KVGLDNLDDLIALRMADRVGNGSRHGMAQPIIQLQEHITRIIEKDNAFSVRDLDINGH
                     DLMTECGCSEGPMIGRVLNHLLELVLEDPELNNNEILKKQALDFIKSMENEESQS"
     gene            complement(1161..2558)
                     /locus_tag="P5123_10160"
     CDS             complement(1161..2558)
                     /locus_tag="P5123_10160"
                     /inference="COORDINATES: protein motif:HMM:NF014910.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-linked oxidase C-terminal domain-containing
                     protein"
                     /protein_id="HRX16669.1"
                     /translation="MYTKPTENDISFFKETLGDDQVITSTDDIKPYTIDKTKNLVGTA
                     SVVLKPCSTEEISKLLGYCSQKSLAIFTRGGGTGVTGGAVPLSDGVVLSLERMNRILE
                     FDAKNMTLLVEPCVITGNIHGFAQEKGLLYPPDPASSDECAIGGNIAENAGGPRAVKY
                     GVTGNYVLGLEYVLPDGTIASSGGKYVKNAAGYDMVRLLTGSEGTLAVITKALLKLVP
                     APPFAQDVLLSFNTLDEAVDTVTRLLAASPLPAAIEFMENDAIKLVSESSSIKIPFAT
                     AGAHLLIKLEADSAQLLKKQIDNLYKIPGISDSNLKTAKTDKESEYLWSARRGIRDAI
                     SRVSPVFFAEDCVVPRAAIALFIKSIKNELSSYGIDSLLFGHAGDGNIHIDILKGKTE
                     DNRWEELKDELRLMIYRKSLAFKGTITGEHGIGFIRKQYLEEAIGSGAYNMMKKIKEA
                     FDPKGILNPGKIFQD"
     gene            complement(2561..3853)
                     /locus_tag="P5123_10165"
     CDS             complement(2561..3853)
                     /locus_tag="P5123_10165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013907283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IMP cyclohydrolase"
                     /protein_id="HRX16670.1"
                     /translation="MSTKDIKQSYKTIMDDSFPDTLTISFGTTQLTYKKRSWKIEEDG
                     ILIEKGLRYGENPGQEAALYQLVNGNLTIGGCNYIEPGNALVSSLTEENLIQFGKHPG
                     KTNLTDIDNSLNILKYLTGKPTTVIVKHNNPSGVAQADNTAESFTKALMADRVAAFGG
                     CVALNTQLDRETAQLISQNYFEVVCAPSFADGAVDILKARKNLRIVEISRMTDLAGFR
                     GSRFIDFKSLMDGGLIIQQSPLNAIKSKDDLIHAATEYKGKKYSIERMPTESEYADML
                     FGWSVEQGITSNSVIYVKDGVTVGIGTGEQDRVGVAQIAVYKAYQKFADAKAFEKYGR
                     PYNQIKLEVEKGEEDKSVIEEIDSITEEARGGIPGSCMVSDAFFPFRDGVDVGIKEGI
                     TSIIQPGGSMLDHQSIEACNEADPKVTMVYTGQRCFRH"
     gene            3994..4833
                     /locus_tag="P5123_10170"
     CDS             3994..4833
                     /locus_tag="P5123_10170"
                     /inference="COORDINATES: protein motif:HMM:NF039204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5715 family protein"
                     /protein_id="HRX16671.1"
                     /translation="MKKTKIFLISFFIGALAAGGSVLFVSRLIHNQRLELQRGAIGAD
                     IADFNRLAYKVERSVVDNVPIYSDYANSALEKKLRSYLYPYHIKIASKFADSVKDLDQ
                     AQKLIESGKLVPVEESLWYYFYGVKKEHRYLVPTALKALDIVAERFNSNLKSRGVDVT
                     VKLAVSSLLRPEDYQMELTGKNSNAVDESTHSFGVSFDLFFDDYYVVLIEGSSGVSGD
                     IINEIRPRYGFMLGDSLRRQFKAILAETMLELQDEGLVYVIMEFRQRVFHVTFTEKLQ
                     SAK"
     gene            4869..5630
                     /locus_tag="P5123_10175"
     CDS             4869..5630
                     /locus_tag="P5123_10175"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025033.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HRX16672.1"
                     /translation="MSENCSSILSEPGNAYTRLAEHYDYMLNHVDYVKWYTYIKTLMY
                     LYYGYPDTILEIGTGTGRFGGFFSRDNYNIIGMDLSLDMLRVAKMRAFNNFRVFCGDA
                     RSFSLNTNIDFIFSVHDTFNYLLTKKELLAAFNCVANCMHAKSVFLFDLTTEYNIKEN
                     FVDDEQTFKHGGSAIVWKNSYDNRDKVIRSIITFQNGKDFHCEEHLQRVYTKKEIVPI
                     LEESGLYLVDQFADYHFDPPTRKSIMENYIVKKRE"
     gene            5632..6024
                     /locus_tag="P5123_10180"
     CDS             5632..6024
                     /locus_tag="P5123_10180"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRX16673.1"
                     /translation="MGYFFTMAGIVIAVTVFVYFINAVDEGKRKLGEITEPTEIASRG
                     SHKVPGENVPGASVTVKRSTALDSLSSEQKDMIPQRQCPLCRTVLNRDQPLYAGNVER
                     DGKRTILIYGCQFCYKEKKEERENRDNV"
     gene            6074..>6405
                     /locus_tag="P5123_10185"
     CDS             6074..>6405
                     /locus_tag="P5123_10185"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRX16674.1"
                     /translation="MKMFFENSREIGKKDTVLVFTYEGFDFKTLPTKMEFMRTYGTAL
                     YFSELKNPLYLPSDKEADLIVVPLKNGDVIELETIRSASAQAARVAMQHKNSAIKIVL
                     PEIDNISR"
BASE COUNT         1730 a         1273 c         1461 g         1941 t
ORIGIN      
        1 catggcacgg ttacggtttt tattgaccgg atacctttcg aaataacaac ttatcgttgc
       61 gatggaaaat atgtggacgg ccgtcgtccc gagactataa gtttctcgga gacactggaa
      121 gaggatattg tgcgtcgtga ctttactatt aatggtcttg catacgatat gcgtgacggt
      181 acaattatcg attatgttga agggctaaaa gatataaaac gaggtttgat aaccactatc
      241 ggtgaccctc ttgaaagatt atccgaagat ggtctgcgtt catatagggc gtgtcgtttt
      301 gctgccaaac ttaatttcca aattagtccg gaaactctta ccggtataac acagactctt
      361 gaaatatcgg caaaggtgtc ggttgaacgc attcgcgatg aaataatgaa gatgatgggg
      421 acaaagacac catctatcgg tatcgaatac atgcgtgaaa caggactttt aaaacaggct
      481 ttgcctgaac tggatgcctg ttacggggta gaacagaacc gatttcataa atacgatata
      541 tattaccatt gcgtctactc ctgtgacgcc gcctcgcctg gtgatcccct tataagattc
      601 gcgtcactta tgcatgatgt tgggaaagtt ccatgtcggg gagtaggtgc cgacggcgat
      661 tttacttttt ataaccacga gattgtaggc gcccgtatga tccgagctct tatgcgaaga
      721 ttaaagtttt ccaatgatga tatcaaccgg gttgttaatc ttgttagcaa ccacatgttt
      781 tattatacag acgagtggac agacggagcc gtcagacgtt ttattagaaa agttgggctt
      841 gataaccttg atgatctgat cgctttaaga atggccgatc gagttggtaa tggatcgcgg
      901 catggaatgg cgcagccaat aatacaactt caggaacata taactcgcat tattgaaaag
      961 gataacgcct tttcggtcag agatcttgat ataaatgggc acgatcttat gaccgagtgc
     1021 ggatgctccg aaggacccat gattgggcgt gttcttaacc atctgcttga acttgtactt
     1081 gaagatcccg aacttaacaa taacgagatt cttaaaaagc aggcactcga ttttattaaa
     1141 tccatggaga atgaagagag tcagtcctga aagatcttgc cgggattgag tatacctttg
     1201 gggtcaaagg cctcttttat ctttttcatc atgttataag cgccgcttcc gatggcttct
     1261 tccagatact gttttctaat aaaacctatt ccatgttctc ctgtgattgt acctttgaag
     1321 gcgagggatt ttcggtaaat cattagccga agctcatcct tgagctcttc ccatctattg
     1381 tcttcggttt tgcctttcaa aatatcgata tgaatattgc catcacctgc gtggccgaaa
     1441 agaagggagt ctattccgta agagctcagc tcatttttta ttgattttat aaatagggca
     1501 atagccgcac ggggaacaac gcagtcttcc gcgaaaaaaa ctgggcttac cctgcttatt
     1561 gcgtctcgaa taccgcgcct tgccgaccat agatattctg actctttatc ggttttagcg
     1621 gttttaaggt tcgagtccga tatgcccgga attttgtaca ggttatctat ctgctttttt
     1681 agaagttgag ccgagtccgc ttcaagtttt attagaaggt gtgctcctgc cgtggcaaac
     1741 ggaattttaa tgctgctgct ttccgatacc agttttatcg cgtcgttttc cataaattcg
     1801 atagcagcag gcagagggga ggccgcgagc agacgggtta ccgtgtcgac agcctcgtct
     1861 agagtattaa acgacagcag tacatcctgc gcaaagggcg gagcaggaac aagtttaaga
     1921 agagccttcg ttattacggc gagagttcct tcgcttccgg taagaagtct gaccatgtca
     1981 tagcccgccg cgttttttac gtatttgccg ccgctcgaag cgatggttcc gtcaggcagt
     2041 acatattcca atccaagtac atagttcccg gttactccgt attttacagc ccgcggccca
     2101 cctgcatttt cggcaatatt gccgcctatt gcgcattcat cggatgatgc cggatccggt
     2161 ggatacaata gtcctttttc ttgagcaaac ccatgaatgt tgcctgtaat tacgcagggt
     2221 tccactaaca gggtcatatt tttggcatca aattccagta tgcggttcat acgttcaagt
     2281 gaaagtacaa caccgtcact aaggggaaca gctccaccgg tgactcctgt gcctccacca
     2341 cgtgtaaaaa tcgcaagaga tttttgtgaa cagtatccga gcagttttga tatctcttca
     2401 gtcgaacatg gttttaatac gacagatgct gtgccaacaa ggtttttggt cttgtcgatg
     2461 gtataaggtt taatgtcatc cgtggatgtt ataacctggt cgtcccccaa ggtttcttta
     2521 aagaaagaaa tatcgttttc ggtcggtttt gtgtacatga ttaatgccta aaacagcgtt
     2581 gtcctgtata taccattgta actttgggat cagcttcgtt acatgcttca attgactgat
     2641 gatccagcat tgatccgccg ggctgaatta tggatgttat accctctttg attccgacat
     2701 ccacaccatc ccggaaagga aaaaatgcgt ctgaaaccat acaggagccc ggtattccgc
     2761 ctcttgcctc ttctgttatg gagtctatct cttcaataac ggacttgtcc tcttcacctt
     2821 tttcgacttc gagctttatc tggttatatg gtcggccata cttttcaaag gcttttgcat
     2881 cggcaaattt ctgatatgcc ttgtataccg caatctgggc aacacccact cgatcctgtt
     2941 caccggttcc tattccgaca gtaacgccgt ccttgacata aattaccgag ttagatgtga
     3001 taccttgttc aaccgaccat ccaaaaagca tatctgcgta ttcggattcg gtcggcatac
     3061 gttcgatgct atatttttta cccttatatt cggttgcggc gtgaataaga tcgtctttgc
     3121 ttttgattgc attcaaaggg ctctgttgta taataagacc gccgtccata agtgatttaa
     3181 agtctataaa gcgtgagcct ctaaatccgg caaggtcggt cattctggat atctcgacaa
     3241 tccgcaggtt ttttcgtgct ttaagaatat caaccgcacc gtcagcaaaa gagggtgcac
     3301 agacaacctc aaaataattt tgggatatca gctgggctgt ctctcggtca agctgggtat
     3361 taagggcaac acaaccccca aaggctgcga cacggtcggc catcagggct tttgtaaatg
     3421 attcggcagt attgtcggcc tgtgcaactc cgctggggtt attatgtttt acgataactg
     3481 ttgttggttt gccagtaagg tatttaagga tgttgagtga attatcaatg tctgtaaggt
     3541 tagttttgcc tggatgcttg ccgaactgaa taaggttctc ttcggttaat gaagatacca
     3601 gtgcgtttcc aggctcaata tagttgcacc ctcctatagt gaggtttccg tttacaagct
     3661 ggtagagcgc ggcttcctgt ccggggtttt ctccgtatcg gagccctttt tcgataagaa
     3721 taccgtcctc ctcaattttc cagctccttt ttttataagt cagctgagtg gtgccaaacg
     3781 aaattgtaag tgtatcggga aaggagtcat ccataatcgt tttgtacgac tgcttaatgt
     3841 cttttgtgct catgttgtct ccgtgatgtt ttttgttaac ggccattttt taaattgttt
     3901 ttgttgaccc ctgtatgtgt caatacatta ttattccctg aatgcagatt aaacggttta
     3961 gtaaagtgct aaccggtaat atcggataag aaaatgaaaa aaacaaagat attcctaatt
     4021 tccttcttta ttggagccct agcagccggt ggttcggtac tgtttgtaag tcgtcttatt
     4081 cataaccagc ggttagaact tcaacggggg gctatcggtg cggacatagc agattttaac
     4141 cgtctagctt ataaagtaga gcgatcggtg gtagataatg ttcctattta cagtgattat
     4201 gcgaatagtg ccctggaaaa aaaactacga agctatttgt atccctatca catcaagata
     4261 gcttcgaaat ttgctgattc cgttaaggat cttgaccagg cacaaaaact aatagagtca
     4321 ggcaaacttg tccctgttga agagagtttg tggtactatt tttacggagt taaaaaagag
     4381 caccgttatc tggtacccac ggcattaaag gctctggata tcgtagcgga gcgttttaac
     4441 agtaacctca agtcaagagg ggtcgatgtg acagtgaaac tggcggtctc atcactcttg
     4501 cgtccggaag attatcaaat ggagcttacg ggcaaaaaca gtaatgcggt ggatgagtca
     4561 actcatagtt ttggtgtgag ctttgatttg ttttttgatg actattatgt ggtgttgata
     4621 gagggtagtt ccggtgtttc cggtgatatt attaatgaga ttcgaccgcg ttatggcttt
     4681 atgcttggag actcacttcg taggcagttc aaggcgattt tagccgaaac catgttagag
     4741 cttcaggatg agggtcttgt ttatgttatt atggaattta gacagagggt ttttcatgtt
     4801 acatttacag aaaagttaca gtcggcgaag taaatatatt gtgattacag atgacactgg
     4861 ggattataat gagtgaaaat tgcagttcca ttttgtccga acccggaaat gcctatactc
     4921 gtctggcgga acattacgat tacatgctta atcatgtcga ctatgtcaag tggtacacct
     4981 atatcaagac tcttatgtat ttatactacg gctatcccga taccatactc gagataggta
     5041 ccggaacagg gaggttcggc ggtttttttt ctcgtgataa ttataatata atcggaatgg
     5101 atctctctct tgatatgttg cgtgttgcca agatgagagc gtttaataat tttagggttt
     5161 tttgcggcga cgcccgatct ttttcgctaa atacaaatat cgattttatc ttttcggttc
     5221 atgacacttt caattatctt ttaaccaaga aagaacttct ggctgctttt aactgtgttg
     5281 cgaattgtat gcatgctaaa tcggttttcc tgtttgatct tacaacagaa tacaatatca
     5341 aagagaattt tgtggatgac gaacagacat ttaaacacgg gggatctgcc atagtatgga
     5401 aaaacagcta tgataatcgt gataaggtaa tccgttctat aataactttt caaaacggga
     5461 aagattttca ttgcgaagag catcttcaac gtgtgtatac caaaaaagaa attgtgccga
     5521 tacttgaaga gagcgggctt tatctggtgg atcaattcgc agattaccat tttgacccgc
     5581 ctacgcgtaa aagtatcatg gaaaactata ttgtaaagaa aagggagtaa gatggggtat
     5641 ttttttacga tggcaggaat agttattgcc gtaactgttt ttgtttattt tattaatgct
     5701 gttgacgaag gaaagcgcaa acttggtgaa ataactgaac cgacagagat tgcgtcaagg
     5761 ggaagtcaca aggtgccggg tgagaacgtt cctggggctt cggttacagt gaaaagaagc
     5821 acggcacttg attctttgag cagcgaacaa aaagatatga ttccgcagcg acagtgcccg
     5881 ttatgtagaa ctgttttgaa tagggatcaa cccctgtacg cgggtaacgt cgagcgtgac
     5941 ggtaaacgaa ccattctgat ctatggttgt cagttctgtt acaaggaaaa aaaagaggaa
     6001 agagaaaaca gggataatgt ttaactctat cgtgatgtgt ctatcaagaa taatgcaatt
     6061 aggtgataat tagatgaaaa tgttttttga aaatagccgt gagatcggta aaaaagatac
     6121 tgtacttgtc tttacttatg aaggattcga ctttaaaact ctaccaacca aaatggagtt
     6181 tatgcgtacg tatggaacag cactttattt cagtgaacta aaaaatcctc tttatctgcc
     6241 atctgataaa gaggcggatc tgatagttgt acccctgaaa aatggagatg ttattgagct
     6301 tgagacaatc cgttccgctt cggcacaggc tgcccgcgtt gccatgcagc ataaaaatag
     6361 tgccataaag attgttttgc cagaaattga caatatctcc agaga
//
LOCUS       DAOTGZ010000164         6402 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_3064
            NODE_14379_length_6402_cov_5.087285, whole genome shotgun sequence.
ACCESSION   DAOTGZ010000164 DAOTGZ010000000
VERSION     DAOTGZ010000164.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868088
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 6402)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6402)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:22:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,785
            CDSs (total)                      :: 2,751
            Genes (coding)                    :: 2,732
            CDSs (with protein)               :: 2,732
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 12 of 19
            Pseudo Genes (internal stop)      :: 5 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6402
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14379_length_6402_cov_5.087285"
                     /isolate="AMR_MDS_3064"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            537..827
                     /locus_tag="P5123_10190"
     CDS             537..827
                     /locus_tag="P5123_10190"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRX16675.1"
                     /translation="MAVFFGPFFFAIEKERTTPCFVKNKKHSVREKKKGRKKERTESL
                     LLCKYQLYGFNEQLLDFLPNKKNSSKKFNKNTFNPVYIYRNILEAQNGSYRF"
     gene            808..1290
                     /locus_tag="P5123_10195"
     CDS             808..1290
                     /locus_tag="P5123_10195"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRX16676.1"
                     /translation="MEVTDFRTSIDVEVCRLDRIVEYAEKHKMDISDVMNTVLDMYSK
                     KLAKNPVCNNTLNRYHERGTIYKSVHIRFSSISKRKLKDIANLYCYSLSYMLFLAMDF
                     FEEFMDGVKEFVRPCFHAAFVHEFFGFTYFINSWGMNFYEGQDNPSGQQSREVTPLLC
                     "
     gene            complement(1379..1452)
                     /locus_tag="P5123_10200"
     tRNA            complement(1379..1452)
                     /locus_tag="P5123_10200"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1416..1418),aa:Arg,seq:tcg)
     gene            complement(1468..1540)
                     /locus_tag="P5123_10205"
     tRNA            complement(1468..1540)
                     /locus_tag="P5123_10205"
                     /product="tRNA-His"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1505..1507),aa:His,seq:gtg)
     gene            complement(1562..1635)
                     /locus_tag="P5123_10210"
     tRNA            complement(1562..1635)
                     /locus_tag="P5123_10210"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1599..1601),aa:Arg,seq:tct)
     gene            complement(1692..1765)
                     /locus_tag="P5123_10215"
     tRNA            complement(1692..1765)
                     /locus_tag="P5123_10215"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1729..1731),aa:Pro,seq:tgg)
     gene            complement(1906..2712)
                     /locus_tag="P5123_10220"
     CDS             complement(1906..2712)
                     /locus_tag="P5123_10220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011555810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ion transporter"
                     /protein_id="HRX16677.1"
                     /translation="MNNKKIRNTLHDIIFKSDSFLGRLFDIVLLWLILGSIVLLMMQS
                     VESIAQKWGDIFRGVEYFFTSIFIIEYILRIYSSKRIRSYILSFFGIIDFVSIVPTAI
                     ILCLGGVQYLFVIRAFRLLRVFRIFKLSKYLHEGEVLARALKASRYKITLFIGVVLSS
                     VIIMGTVMFVVEGAENGFTSIPLSIYWAIVTMTTVGYGDIAPQTIAGQIISSIIMILG
                     YAIIAVPTGIVTVELSQGSQINETICPRCGLNKHDKDARFCKRCGERISR"
     gene            complement(2729..4141)
                     /locus_tag="P5123_10225"
     CDS             complement(2729..4141)
                     /locus_tag="P5123_10225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010528054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MATE family efflux transporter"
                     /protein_id="HRX16678.1"
                     /translation="MIDMIKGNPLGLIVKFSIPMFAGSVFQQLYNVADSVIVGRFLGK
                     NALAAVGASFPIMFLLIALSMGATMGISVMISQYYGARDYKKVKLCIETSYIFLVIIA
                     LIVTVLGLVLDDIIINMLNTPSEIYADTKLYIDILFGGLFLMFGYNSISAVLRGLGDS
                     RTPLYLLIFATVLNIALDLVFVVCFNWGVAGVAVATVLAQGASFIGGILYLQRINPLF
                     AVNYFKLRFDRNIFLKSIKIGLPTGAQQMFVAAGMMALTRIVNQFGTNALAAFTAAAR
                     IDSFAVMPAMNFSLAMSTFVGQNIGAGKLERVNSAIRIGLIFAVVCALTVSVTVICLS
                     NQLMLLFTTDAEVISIGREYLFIVSGFYIAFMIMFIFNGALRGAGDTLIPMFITLFSL
                     WLIRIPVSTYFSSKMGTRGIWLGIPIAWVLGAIFSCIYFLTGRWRRKSIVHPGNVDVD
                     SVELVNDIECLEMEKKECRL"
     gene            complement(4320..4757)
                     /locus_tag="P5123_10230"
     CDS             complement(4320..4757)
                     /locus_tag="P5123_10230"
                     /inference="COORDINATES: protein motif:HMM:NF012241.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp20/alpha crystallin family protein"
                     /protein_id="HRX16679.1"
                     /translation="MKWGIANRNNDTYDMWSKTFDRIFDDFFSASPVGTSTVPALDIE
                     EEKGRYVLTAELPGMEESDLDISVTDGVLTISGEKKEEKKKEEKCCIVSERRYGSFSR
                     SIPLPKNIDTDKLKAKFEKGVLSIEMPKDEKAAPKKIKILPNK"
     gene            4927..5301
                     /locus_tag="P5123_10235"
     CDS             4927..5301
                     /locus_tag="P5123_10235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020611787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HRX16680.1"
                     /translation="MKIQLDNSSGVPYYRQIIRNIETMKASGRLLPGDKLPTIRALAI
                     ELKINPNTIAKAYNELEIRGIVKTQVGRGTFISEMDPEQDDHKINQEIIDTVKKFLND
                     LRILNVDYNDIPELIRQCQEDK"
     gene            5366..6049
                     /locus_tag="P5123_10240"
     CDS             5366..6049
                     /locus_tag="P5123_10240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013969635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HRX16681.1"
                     /translation="MKCVEISNLTKKYVLNDIIVNALQGVTTSFESGEFTAIAGPSGS
                     GKSTLLHLIGCLDTPSEGSININEADVGIMNRSQLAILRRQSIGFIFQAYNLIPVLSA
                     LENISLPLILLGVDKKQIRSRSLEVLEQVGLENMQDRRPSEMSGGQQQRVAIARALVK
                     KPSLILADEPTANLDSKTGRDILELMYNLNKTENSTFIFSTHDHLVMEYARRLIKLRD
                     GLIDDEVLQ"
     gene            6046..>6402
                     /locus_tag="P5123_10245"
     CDS             6046..>6402
                     /locus_tag="P5123_10245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020611789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HRX16682.1"
                     /translation="MIKNELIMLITMSFRNLGRHKVKTVITSTAIAISVSLYIFMDAW
                     IMGMNIDSRRNIVIYETGAAKIQTRAYFNKKKELPMYESFSDYKKIESVLNNSGYNTA
                     PRFVFSGTIHSGTATAP"
BASE COUNT         2197 a         1323 c         1107 g         1775 t
ORIGIN      
        1 aaataaagaa aacagcatcg taaagacgct cggcaataag gcaatgaata gtgaagagtg
       61 aaaaatgaat aattgtcatt gcacatacat aaataacgta aagtctgaaa ccgtttaaga
      121 caaaatccat atatccagaa tttcagatta cgtgctctga agccggtacg atgcctcaaa
      181 acctcacccc ggctccgccg cgccctctcc gattcggaga gggcttcgta tacaaaattc
      241 acgatgaata cgtaataagt attttaacgt tatctattcc gccttgcaga tattctatac
      301 tgcaacacaa ccaatgatta cgcaaagtct aaaaccgttt aagacaaaat ccatatatcc
      361 agaatttcag attacgtgct ctgaagtcga cacgatgtcg acgctgtagc gattatggca
      421 ggaggccatc atagcggaca ttcaaaagta ggaaatatca ataaagtcac attgaaaaac
      481 cttgcaaagc tgattaataa tccttaccct gcagggtcga ggggcagccg gccctcgtgg
      541 cagttttctt tggtcctttc ttttttgcta ttgaaaaaga aaggacaacc ccttgttttg
      601 ttaaaaacaa aaaacactct gtaagagaaa aaaagaaagg aaggaagaaa gaaaggacag
      661 aaagtttgtt gctctgcaaa tatcaactct acggatttaa tgagcaactg ttagatttct
      721 tgccaaataa aaaaaatagc tcaaaaaaat ttaacaaaaa taccttcaac ccagtataca
      781 tatatagaaa tattctggag gcacaaaatg gaagttacag attttagaac atccatagat
      841 gttgaagtat gcagattgga tagaatagtt gagtatgctg agaagcacaa aatggacatt
      901 tcagatgtaa tgaacacggt tttggatatg tattcaaaaa agctggctaa gaatccagta
      961 tgcaataaca ctcttaatag gtaccatgaa agaggtacaa tttacaaatc agtacatata
     1021 cggttttcaa gcatttcgaa aagaaaactg aaagatatag ccaatctgta ctgttacagc
     1081 ttgtcttata tgctatttct tgcaatggat tttttcgagg agtttatgga tggtgttaag
     1141 gagtttgttc gtccctgttt tcatgctgct tttgttcacg agttctttgg ctttacatac
     1201 ttcataaaca gctggggtat gaacttctat gagggacaag acaatccatc tggacaacaa
     1261 tcccgagaag tgacgccttt gttgtgttaa aatggctttt tctgtgtttt gatcaataaa
     1321 aaacggaaaa ccgaaaggga aaaaagtagg aatgagaatt tcttggagtt tctttatgca
     1381 cgcccggaga gattcgaact cccgacctgc ggattcgaag tccgacgctc tatccagctg
     1441 agctacgggc gcgtataaag aaccatgggt gagtgacggg gcttgaaccc gcgaccacag
     1501 gagccacaat cctgggctct accaactgag ctacactcac cgtaaataaa ctcagaaaac
     1561 gcacgccaga gaggattcga acctctgacc tacggcttag aaggccgttg ctctatccag
     1621 ctgagctact ggcgcgtaat acgcacaact accaaagcag agtgccggaa aagcaagggt
     1681 tgtacgttat gtcggggcga aaggattcga accttcggcc ccctggtccc aaaccaggtg
     1741 cgctaccgga ctgcgccaca ccccgaaaat gttaagccaa gttattatcg gtactgaaag
     1801 ttgtcaaata tattagtatc tcatggaaat attttttcaa ctcactatca aaaaaagtaa
     1861 ttatttaaag tttataatac acttcatctc aaaaccatca taggattatc tgcttattct
     1921 ttcaccacat cgcttgcaaa atctggcatc tttgtcatgt ttgttaagac cgcatcttgg
     1981 acagatagtt tcattgattt gagaaccctg actcaattca acagtgacaa ttcctgttgg
     2041 tacagcaatt atggcgtatc ccaaaatcat tattattgat gaaataatct ggccagcaat
     2101 tgtctgtgga gctatatctc catatcctac cgttgtcatt gtaacaattg cccaataaat
     2161 acttaagggg atactagtaa atccgttttc ggcaccctcc actacaaaca ttactgtccc
     2221 cataattatg acggaactca aaactacccc aataaacaga gttatcttgt aacgactggc
     2281 tttcaaagca cgagccaaaa cctctccttc atgtaaatac ttcgaaagct taaaaattct
     2341 aaacaccctg agcaatctaa aggctcttat aacaaaaaga tattgaacac cacctaaaca
     2401 cagaattatt gcggtcggca caatcgatac aaaatcaata atgccaaaaa aggataagat
     2461 ataacttctt atcctttttg aactatatat tcttagaatg tattctatta taaaaattga
     2521 agtgaaaaaa tattcaacac ctctgaagat atctccccat ttttgtgcta tcgattctac
     2581 actttgcatc atcaatagca ctatacttcc taaaatcagc cataacagaa caatatcaaa
     2641 caatcgccct aaaaatgaat ccgatttaaa aattatatca tgcagcgtat ttctaatttt
     2701 tttattattc atttcacaac aaccttaact acaacctaca ctcttttttt tccatttcaa
     2761 ggcactctat atcattcaca agttcaactg aatccacatc cacattgcca ggatgaacaa
     2821 tagattttct acgccaccga ccggttagaa aatatataca agaaaaaatt gcaccaagca
     2881 cccatgctat agggattccc aaccagatcc cccttgtccc cattttgctt gaaaaatatg
     2941 tagagaccgg aattctgatt aaccataaag agaataaagt aataaacatc ggtatcagtg
     3001 tatcaccggc tccgcgaagc gctccgttaa agataaacat aatcataaag gcaatataaa
     3061 acccgcttac aataaaaaga tattcccgac ctatcgaaat tacttccgca tctgtagtaa
     3121 aaagtagcat taactgattg cttaaacaga ttacagttac cgaaactgtt aaagcacata
     3181 ctaccgcaaa tatcaaaccg atacgtatgg cagagttaac ccgctcaagt ttaccggcac
     3241 caatgttttg accaacaaaa gtagacattg ctaaagaaaa attcattgct ggcataaccg
     3301 caaatgagtc aattcgagct gccgcagtaa aagccgcaag cgcgtttgtt ccaaactggt
     3361 taacaattct ggttagagcc atcattcctg cagcaacaaa catctgctgc gcgccagtag
     3421 gaagccctat ttttatactt tttaaaaaga tatttcgatc aaaacgcaac ttaaaataat
     3481 tcacagcaaa aagcggattt attctttgca gatataaaat tccacctata aaagaggctc
     3541 cctgagccaa aactgtagca accgcaactc cagcaacccc ccagttgaaa caaacaacga
     3601 agaccagatc cagagcaata tttaaaacag tagcaaaaat aagtaaatat agaggcgtac
     3661 gcgaatcccc caatcctctt aatactgcgc ttatagagtt ataaccgaac atcaaaaaca
     3721 gtccaccgaa gagaatatca atataaagct tagtatcggc atatatctct gacggggtat
     3781 ttagcatatt tattataata tcatccagaa caagccccag aacagtaact atcaacgcaa
     3841 taattacaag aaatatatac gatgtctcaa tacataattt gacctttttg tagtccctgg
     3901 ctccatagta ctgtgaaatc attacggata ttcccatggt cgctcccatt gaaagtgcga
     3961 tcaaaagaaa cataatcgga aaagaagctc ccacagccgc taaagcattt tttcccaaaa
     4021 aacgaccaac tataactgaa tctgctacat tgtaaagttg ttggaaaacg ctccctgcga
     4081 acatgggaat cgagaatttt acaatcaaac caagcggatt tccttttatc atatcaatca
     4141 tgtttcaaga cctaccctaa gcaatataaa agaacagaac cgatgtccat cagtcatttt
     4201 aattatttgt catgattata tcagaggata acagttcaac aataaaacta ttaataaaaa
     4261 agggctcaca acagattgag agcccttcat taatagtata atttattcac tactataggt
     4321 catttgtttg gaagaatttt aattttttta ggagctgctt tttcatcttt tggcatttct
     4381 attgatagca cacctttttc gaattttgcc tttagtttgt ctgtgtcgat attttttggt
     4441 aaaggtattg atcttgaaaa cgagccataa cgtctttccg atacaatgca acacttctct
     4501 tctttcttct tctcttcttt cttttcaccg gaaattgtca gtactccatc tgtaaccgaa
     4561 atatcaagat cactctcctc cattcctgga agctctgctg ttaaaacata cctgcctttt
     4621 tcctcttcga tatccaatgc aggaactgtc gaggtaccga ccggtgaagc ggaaaaaaag
     4681 tcatcaaaaa tccggtcaaa agttttgctc cacatatcgt aggtatcgtt gtttctgtta
     4741 gctattcccc atttcatggc caacctcctt tattatatta gcactcatta gccctatgtg
     4801 ctaacaataa tttataaaat attgtttaat ccgtcaacat gtcttaataa atttttaaaa
     4861 aaatattgac ctattgtact attacaatta tatttgtact atgttaatag tacaaggtaa
     4921 tgattaatga aaattcagct cgataattcg tcaggtgttc catattaccg acaaattata
     4981 agaaatatcg aaacaatgaa agcgtcagga aggctattgc cgggtgataa actacccaca
     5041 attcgtgctc ttgcgatcga gctaaagatt aatccaaata caatcgcaaa agcttataac
     5101 gaacttgaaa tcaggggtat tgttaaaacc caagtggggc gtgggacctt tatttcagag
     5161 atggaccccg aacaggatga ccataaaata aaccaggaaa taatcgatac tgttaaaaaa
     5221 tttcttaacg acttaagaat actcaatgtg gactataatg atattccgga actgatccgg
     5281 caatgccagg aggataaata atgtttacta ccaaaagaga aaaaaacaga cacataaaaa
     5341 ataatatttt gtactggagt aaaaaatgaa atgtgtagaa ataagcaatc ttactaaaaa
     5401 atacgtttta aatgatataa tagttaatgc tcttcaagga gtaacgacat cttttgagag
     5461 cggagaattt acagctattg ccggaccatc cggaagcggc aaatctacac ttttgcatct
     5521 aataggttgc ctagatacac cgagtgaagg cagcataaat atcaatgaag cggatgtcgg
     5581 aataatgaac cgttcacaac ttgccattct aagacggcaa tcaattggat ttatttttca
     5641 ggcatataat ctgatccctg tactatccgc gttagagaac atctcccttc ccttgattct
     5701 tcttggtgtc gataaaaaac agataagatc acgttctctg gaggtcttag agcaagttgg
     5761 tcttgaaaat atgcaagatc gtcgcccttc cgaaatgtca ggcggccaac aacaacgtgt
     5821 tgcaatcgct agagctctgg taaaaaagcc ctcacttatt cttgccgatg agccaacagc
     5881 caatcttgac tcaaaaacag gaagagacat tctagaacta atgtataatt tgaacaagac
     5941 cgaaaacagc acatttattt tttcgacaca cgaccatctg gttatggaat atgcgcgcag
     6001 attaattaaa ctcagagatg gacttatcga tgatgaggta ctacaatgat taaaaacgaa
     6061 ttgattatgt taatcactat gtcttttcgt aatctggggc gccacaaggt aaaaaccgtt
     6121 attacttcaa cagctattgc gattagtgta tcgttgtaca tatttatgga tgcatggatc
     6181 atgggcatga atattgactc aaggcgtaac atcgttatat atgaaacagg agccgcaaag
     6241 attcaaacaa gagcctattt taacaaaaaa aaagagctac ccatgtacga aagtttttct
     6301 gattacaaga aaatcgagtc ggttcttaat aatagcggtt acaacactgc gcccaggttt
     6361 gtattttcag gaacgataca ctccggtaca gcaacagcac ct
//
LOCUS       DAOTGZ010000165         6378 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_3064
            NODE_14449_length_6378_cov_3.891349, whole genome shotgun sequence.
ACCESSION   DAOTGZ010000165 DAOTGZ010000000
VERSION     DAOTGZ010000165.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868088
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 6378)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6378)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:22:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,785
            CDSs (total)                      :: 2,751
            Genes (coding)                    :: 2,732
            CDSs (with protein)               :: 2,732
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 12 of 19
            Pseudo Genes (internal stop)      :: 5 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6378
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14449_length_6378_cov_3.891349"
                     /isolate="AMR_MDS_3064"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..233)
                     /locus_tag="P5123_10250"
     CDS             complement(<1..233)
                     /locus_tag="P5123_10250"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRX16683.1"
                     /translation="MIQYGHSLFWYRLMMSLGIVAISIFLNLIIVLSGKILKPYYIVL
                     IYLPAILAAGIYFMPGNPLSTVFYQNGYYRYYT"
     gene            889..1380
                     /locus_tag="P5123_10255"
     CDS             889..1380
                     /locus_tag="P5123_10255"
                     /inference="COORDINATES: protein motif:HMM:NF014116.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016798 - hydrolase activity, acting on
                     glycosyl bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate binding domain-containing protein"
                     /protein_id="HRX16684.1"
                     /translation="MEKYFKKALLLLMLVLFVGLTSCDDPAVVGSDDPNYNQLFEQTE
                     FTETVRPWILETDKGSEAKLMELKDGVVHIRVGDNDVKPSFEYGLQFSYPMLEIQKGK
                     TYKLNFDVMIKNKENVSEKTPHKFNVAIAGDRANVPNQYSDVLGHGGDTLTVAENDEW
                     VSY"
     gene            1402..4272
                     /locus_tag="P5123_10260"
     CDS             1402..4272
                     /locus_tag="P5123_10260"
                     /inference="COORDINATES: protein motif:HMM:NF012961.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 9 protein"
                     /protein_id="HRX16685.1"
                     /translation="MSKATDPAAKFYIRFGGQGTNYDIFIRNLSLVSPKTGDNAHVVR
                     IKEETPSSIIRVNQLGYLPNARKRASIVLDNGDPNYVPQVEDVLPQVVVESIDDNGFE
                     YPVSLVRHGYDVTAGEYIAVVDFSNLSKSGSYRIRASYDGQIEYSHDFEISSDLYSQL
                     KNDAIDYFYYNRIGDVSVKHAARPDLAREKGANHAGVFSALTKKDFDNTDFAPAKNDY
                     LQLVKDFGEDFSTSITSGWYDAGDYGLYVVNGGISAWTLLNWYERTKTFGTSADFGFG
                     EGSLRYQSASDKKEDIIEMVDDEIRFMIAMQEKSGEFEGMVHHKIHRLKWSDFPSTPA
                     QDVGERKIFPVSTAATLNLAAVAAQAARIYKGLSVEAKDRNTAESYGQRSSEYLKAAQ
                     KAWDAADKYNKVYAPNPDWKPGGGPYDDTNLSDEFFWAAAELYITTGDEEKYLNKIFQ
                     ERVPSRQALKSCNFGFILDENGEKFYGAFSWKDTAGLGVMSLALAPKGISGYNVNADV
                     QNAAMFRLYQSSDVWLQQLKKHGYGTPLSCVESKKYPDKKDTQYPWGSNSFVMNMGIV
                     FGYMYDFTGEKKYLDAVVEGMDYILGRNAMDISYVTGYGERAFKNPHHRVWANQYGPE
                     YPKPAPGAVSGGPNTGENCPFGYSYLNGQNTPPQKAYIDHIQAWSTNEITINWNAPLV
                     WLTAYLDEAANNREDVEIEICVPRRSWMTGGAMTPEVWSEVPVYINPDKSIEEIQFFY
                     AFDGAESASDQNRYENAEKIEIFSIRPITRSISAPSADFKTDDGNYALFAKVYLNNGK
                     TYIKKVGTLVTKKTAGKSTDAEVYLMYITGGPTDARLKGYRDGDFVPVVANVIPSYRD
                     EELRTAQTCRSIDYYVIKHGDDMQDPSSWAYIGSIDATQEEPDSSQMDYDAYLIDWQL
                     DGPGEYIYVIRTNWVVSWWGQYAGGGIEYSQYDVSPVLNYGE"
     gene            complement(4370..5665)
                     /locus_tag="P5123_10265"
     CDS             complement(4370..5665)
                     /locus_tag="P5123_10265"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRX16686.1"
                     /translation="MRNLVLVITSFITIILADRVYAFPAYDGTFSFSASADNETENDT
                     KLNPGNILDMGYPTGASLNSDIDIRLLMHRNFTFSIANSSYIRYNYYTDKPEDGETWE
                     HDNTLKQLFGTFHAGEHLIIDAGKKTERNGIMFFRNPSDFFQTGSTTGLSRSKEERDA
                     DLEGSVLVKGEIFIGSNVSVNAVFSPEYERVDNSITQIQCGISYQFDAADFTLTGYKG
                     DVYKLGTSASANIGTSAVIRGEASVSEEKNRFTLSKGTTAPNDPNEGIYYKPEESIMR
                     RPWEASVGGQYTFKDGSDLYAEYYYNHAGYTDIEWNDLMKVIDQCSNVSQFYNRISML
                     QLNGVIRDNGGITAARRHYLFFRYSKNDIIFEDFNFAAATVIGLENAGGLLTLSPQYE
                     ISNNTSLKGDATFFGGEDESEFELMYHRMSFEIMIEYVF"
     gene            complement(5666..>6378)
                     /locus_tag="P5123_10270"
     CDS             complement(5666..>6378)
                     /locus_tag="P5123_10270"
                     /inference="COORDINATES: protein motif:HMM:NF028441.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_process: GO:0042953 - lipoprotein transport [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="outer membrane lipoprotein-sorting protein"
                     /protein_id="HRX16687.1"
                     /translation="ITMLLILNVPLAARDANSIVEAADKYRGFKGSFAQQSTAVYYKN
                     DKEESSTNLKVYINDNRNSLVRFMGPGREKGQIMLMSGNNIWMYIPGTRNPIRLSPQQ
                     RLLGQASNGDVARTNYSDDYDAELDGEEKVENINCHRLILTANEPDTTYQKIVYFVKK
                     TDNRPVKALFYAKSGKLLKTGYFKKPGTFLGRTILRELHLYDELKKNEKTVIKVEKIT
                     REKLPASHFNYKYLPRIR"
BASE COUNT         1901 a         1137 c         1395 g         1945 t
ORIGIN      
        1 tctgtgtagt aacggtaata accattttgg taaaaaaccg tggataaagg gttgccgggc
       61 ataaagtata ttcctgcggc taaaatagcg ggtaaataga tcaaaacaat gtaataaggt
      121 tttagaattt ttcccgataa aacaattata agatttaaaa aaatagaaat cgcaacaata
      181 ccgagagaca tcattaggcg ataccaaaaa agagaatgtc catattggat cattattcca
      241 cagaaaatta caacgcttac aacaatacaa aaaataaaaa aatgtagtga ttgtttgttg
      301 ttagtagacc ttaataaaat atagaaacca aaatatattg tgctggtaaa agcaaaaacc
      361 attatcatga tatataatat aaataactgc atataaattc cttataaaat cttgcaataa
      421 gagaagttag gcgaaaatca atataattat aactaatttg aacaaagtaa acacagacaa
      481 gttatgtgca acttaaaatt caatttaatc atatatcagc atgaattttt tttgattatg
      541 attacccata agtccgtatg ggcttgctat cttgcataaa gtacgtacgg gttggttttg
      601 ggcgtaatca tttttttaat aacgttagtt tatagtgcgt ataacctttc tgttttgcaa
      661 gtagcgttaa aaatattcgg tttttgtatg cttttaagta tttgtacgga tgacaaaatt
      721 ctcctttttg tgatataaaa agcaaaagtt atgtgacaaa caatgaacaa catcaaattt
      781 ctggcagata aggtgatttc ttatagagcc taatcttaaa gaagtttcaa gcgttccttg
      841 ttacgtgctt cattgtttta gattattaca aataattagg agagaattat ggaaaagtat
      901 tttaaaaaag cactattatt gttaatgttg gtgctttttg ttggtctcac ttcttgtgat
      961 gacccggcag tggtggggag tgacgatccc aactacaatc aactgtttga acagacagaa
     1021 tttacagaaa ctgttcgccc ctggattctt gagactgata agggctcgga agctaaatta
     1081 atggaactaa aagacggtgt tgttcatatc cgtgtcggtg ataacgacgt aaaaccgagt
     1141 ttcgagtatg gcttgcagtt ttcttatcca atgcttgaaa ttcagaaggg aaagacttac
     1201 aaactaaatt tcgatgtaat gataaagaac aaagaaaatg taagtgaaaa aacaccgcat
     1261 aaatttaacg tagctattgc cggtgacaga gctaatgtac ccaaccagta tagtgacgta
     1321 ttgggacacg ggggcgatac cttaactgtt gctgagaatg atgaatgggt ctcttattag
     1381 ggatctttta atgaatttac catgtccaag gctacagatc cagcggcaaa attctacatt
     1441 cgttttggag ggcaggggac aaactatgac atttttattc gtaatttgtc actggttagc
     1501 ccaaaaactg gtgataatgc gcacgttgtt cgtattaagg aagaaacgcc atcttcgatt
     1561 attcgtgtta accagcttgg ctacctgcca aacgcacgta aaagagcttc aatagtactt
     1621 gacaacggag atccgaatta tgttccacaa gtcgaagatg tattgccaca ggttgttgtt
     1681 gaatctattg atgataatgg tttcgaatat ccggtttctc ttgtgcgtca tggttacgat
     1741 gttactgccg gtgaatatat cgcggttgta gatttttcta atttatccaa atctggaagt
     1801 tatcgaatcc gtgccagcta tgatgggcag attgaatatt cacacgattt tgagatcagt
     1861 agtgatctat attcacagtt gaagaacgat gcaattgatt acttttatta taacagaatt
     1921 ggtgatgttt cagtaaagca tgctgctcgc ccagatttgg cgcgtgaaaa gggcgctaat
     1981 catgcagggg tcttctccgc gcttacgaaa aaagacttcg ataataccga ttttgctcca
     2041 gctaaaaatg attatttaca acttgttaag gattttggtg aagatttttc aacatccatt
     2101 acttctggat ggtacgatgc aggtgattac ggactttatg tcgttaacgg tggaatatcg
     2161 gcctggactc ttttaaattg gtatgaacga accaaaactt ttggcacgag tgccgatttt
     2221 ggatttggtg aaggttcact acgttatcag agtgcatcag acaaaaaaga agatattata
     2281 gaaatggttg atgatgaaat acgtttcatg atagctatgc aggaaaagtc tggagaattt
     2341 gaaggaatgg tgcaccataa aattcatcgc ttaaaatggt ctgattttcc tagtactccg
     2401 gcacaggatg tgggagaacg gaaaattttc cctgtaagta ctgcggcaac tcttaatctt
     2461 gcggcagtag cagctcaggc agcacgaata tacaaagggc tatctgttga ggcaaaagat
     2521 agaaatacgg cagagtcgta tggtcagcga tcttcagagt atttgaaagc agctcaaaag
     2581 gcttgggatg cagctgataa atacaataaa gtttatgccc caaatccaga ctggaaacca
     2641 ggcggcggtc cttatgatga tactaattta tcagatgagt ttttctgggc agcagccgag
     2701 ctttacatca ctactggtga cgaggagaaa tacctgaata agatatttca ggaaagagtt
     2761 ccatcgcgac aggctcttaa gtcttgtaat tttggtttta tcctggatga gaacggagaa
     2821 aaattttacg gagctttctc ttggaaagac accgccggat tgggcgtaat gtctctggct
     2881 cttgctccta agggaatatc cggatataat gttaatgctg atgttcagaa cgctgcaatg
     2941 ttcaggttgt atcagtcgtc agacgtgtgg ttacaacaac tgaaaaagca cggatatgga
     3001 acgccattaa gttgtgttga atcaaaaaaa tatcctgata aaaaggatac ccagtatccg
     3061 tggggctcaa actcattcgt tatgaatatg ggtattgtgt ttggttacat gtatgatttt
     3121 acaggagaaa agaagtatct tgatgctgtt gtagagggta tggattatat tcttgggcgt
     3181 aatgccatgg atatctctta tgtaacaggt tatggtgagc gtgcttttaa gaatccgcat
     3241 catcgtgtgt gggccaatca atatggacca gaatacccta aacctgctcc aggtgcggtt
     3301 tccggtggac caaacactgg tgagaattgc ccttttggat attcatatct taatggtcag
     3361 aacactccgc ctcaaaaagc atacatcgac catatacagg cttggtctac taatgaaatt
     3421 acaattaact ggaacgcccc gcttgtatgg ctaactgcct atcttgatga agcagctaat
     3481 aatagagaag atgtagaaat agaaatttgt gttccacgtc gatcatggat gactggtggg
     3541 gctatgactc ctgaggtatg gtcagaggtt ccggtttaca ttaatccaga taaatctata
     3601 gaagaaattc agtttttcta tgcttttgat ggcgccgaga gtgcttcgga tcaaaacaga
     3661 tacgagaatg ctgaaaaaat cgaaattttt agtattagac cgataacacg ttctatttcc
     3721 gcgccatccg ctgattttaa aacggatgat gggaattatg cgctttttgc taaggtatat
     3781 ttaaataatg gtaagactta tataaagaag gtcggaacac ttgtcacaaa aaaaacagct
     3841 ggtaaaagta cggatgccga ggtttatctg atgtatatca caggcggtcc aactgatgcc
     3901 agactaaaag gttaccggga tggcgatttt gtacctgttg tggctaatgt aataccaagc
     3961 tatcgtgatg aagagttaag aaccgcccag acctgtagga gtattgatta ctatgtaata
     4021 aagcatggtg atgatatgca ggatccatcc tcatgggctt atatcggatc aatagatgca
     4081 acgcaggaag aacctgatag tagccagatg gattacgatg cttacctgat cgattggcaa
     4141 ctggatggac ccggagagta tatttatgta attagaacca actgggtagt tagttggtgg
     4201 gggcagtatg ccggcggggg tattgaatat agccaatacg acgtcagccc tgttttaaac
     4261 tatggagaat agagttagaa ttgtataaat aattttattc gattacttgt cgctataata
     4321 aggggagcct tcgggttccc cttagcctta aagatatgcc taaaaaattt taaaaaacat
     4381 actctatcat tatttcaaaa ctcattctat ggtacatgag ttcaaactct gattcatctt
     4441 ctcctccaaa gaaggttgcg tcgcctttta atgaagtatt gttagatatt tcgtattgtg
     4501 gtgataatgt aagaagtcct cccgcgttct ccaaaccaat aaccgtggca gctgcgaaat
     4561 tgaaatcttc aaaaataata tcatttttac tgtaacggaa aaaaaggtag tgccgtcttg
     4621 ctgctgttat gcctccgttg tctcgtataa cgccgtttag ctgaagcatg ctgattctgt
     4681 tataaaactg agatacgtta gagcattggt cgatgacttt cataagatcg ttccattcga
     4741 tatcagtata accggcgtgg ttgtagtagt actcggcata gaggtcggag ccatccttaa
     4801 atgtatattg tcctccgacc gaggcttccc atgggcggcg cataatgctc tcttccggtt
     4861 tgtaatagat gccttcgttc gggtcgttgg gggcagttgt acctttggat aatgtaaacc
     4921 tgtttttctc ttctgaaaca gaagcttcac ctctgataac cgcagatgtg ccaatattgg
     4981 cagatgcgga agtgcccagt ttgtaaacgt ctcccttgta tccggttaac gtaaagtcgg
     5041 cggcgtcaaa ctgatacgaa ataccgcact gtatctgggt tatactattg tcaactctct
     5101 catattcagg agaaaagaca gcgttaacag aaacattgct tccgataaaa atttctccct
     5161 ttacaagtac actgccttca agatcggcgt cacgctcctc tttcgaacgt gaaagcccgg
     5221 ttgtcgatcc tgtttgaaaa aaatcagacg gatttctaaa aaacataata ccattacgtt
     5281 cggttttttt tccggcgtca atgataaggt gctcgccggc atgaaaggtt ccgaacagct
     5341 gtttgagagt attgtcgtgc tcccatgttt cgccatcttc aggtttgtca gtataataat
     5401 tataacgtat gtagctgctg ttggctatcg aaaaagtaaa gttcctgtgc atcagaagtc
     5461 tgatgtcaat atcgctattc agtgacgcac cggtaggata acccatgtcc agaatattgc
     5521 cgggattaag cttggtatcg ttttctgtct cgttatctgc agatgccgaa aatgaaaaag
     5581 taccgtcgta tgccggaaag gcgtatactc tgtccgccag gatgatagtg ataaaggaag
     5641 ttataacaag tacaagattt ctcatttagc gtatccttgg aagatattta taattaaaat
     5701 gtgatgcagg aagtttttcc cgggttattt tttcaacttt gatcactgtt ttttcatttt
     5761 ttttcagctc atcgtaaagg tgaagttccc ttagtatggt tcggcctaaa aatgtgcccg
     5821 gttttttaaa gtagcctgtt ttcagaagct ttccgctttt tgcataaaag agtgctttaa
     5881 cagggcgatt gtctgttttt tttacaaaat agactatttt ctgataggtt gtatccggct
     5941 cgttcgctgt gagtattaac cggtggcaat ttatgttttc gactttttct tctccgtcaa
     6001 gttctgcgtc ataatcatcg ctatagttag ttctcgcgac gtctccgtta gaagcctggc
     6061 ctaataaccg ttgctgcgga gagagtctta tagggttgcg tgttccggga atatacatcc
     6121 agatgttatt gccgctcata agcataatct gccccttttc gcggccgggc cccataaagc
     6181 ggacaagtga gttgcggtta tcgttaatgt acactttgag gttggttgat gactcctctt
     6241 tgtcattttt ataatataca gcagtagact gttgtgcaaa actgccttta aagccacggt
     6301 atttgtcggc tgcttctaca atactgttag cgtcacgcgc cgcaagtggc acattcagaa
     6361 tcaatagcat cgtaatca
//
LOCUS       DAOTGZ010000166         6353 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_3064
            NODE_14530_length_6353_cov_5.798349, whole genome shotgun sequence.
ACCESSION   DAOTGZ010000166 DAOTGZ010000000
VERSION     DAOTGZ010000166.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868088
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 6353)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6353)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:22:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,785
            CDSs (total)                      :: 2,751
            Genes (coding)                    :: 2,732
            CDSs (with protein)               :: 2,732
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 12 of 19
            Pseudo Genes (internal stop)      :: 5 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6353
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14530_length_6353_cov_5.798349"
                     /isolate="AMR_MDS_3064"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..448)
                     /locus_tag="P5123_10275"
     CDS             complement(<1..448)
                     /locus_tag="P5123_10275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006869936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GIY-YIG nuclease family protein"
                     /protein_id="HRX16688.1"
                     /translation="MADKYFPSRPEVDPRIYAYMDTNPQYDGLLKIGYTTIDVKARVS
                     QQYPILRPGEKPYKILLSEPAIRNDGTSFTDHDVHKYLKRRGFENPNGEWFKCSVDDV
                     KNAIIGIKERRYVEDDRSLSFSLRPEQKTAIEKTINYIKDFRKENPN"
     gene            complement(451..1947)
                     /locus_tag="P5123_10280"
     CDS             complement(451..1947)
                     /locus_tag="P5123_10280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013685368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0006304 - DNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Eco57I restriction-modification methylase
                     domain-containing protein"
                     /protein_id="HRX16689.1"
                     /translation="MLTDSYNPDVLSCIANLSSDEVFTPPKLANEMLDLLPDEIWHDS
                     TIKFLDPFTKTGVFLREITQRLMEGLKTKIPDVQERADHILKNQVYGIAITELTALLS
                     RRTVYCSKKANGDYSVCSSFNDEQGNILYNKTEHGWENGRCAFCGASQSIYDRVPELE
                     THAYQFIHTYKPEEIFNMKFDVIVGNPPYQMTDGGGTGSSARPIYNYFVEKALELNPS
                     FLCMIIPSRWFSSGKGLDCFRNKMLNDKRVKYLIDYSDSRDCFPSVDIAGGVCYFLWE
                     KDYHGKCTVINFNKGTSTKTIRPLNEFDIFIRDGIAVSIIHKIREKSREYLDSIVSSR
                     KPFGLESKDRPTKNGDLTLIWSGGDGPFPREKVKSGHNYIDKYKVLLSKASFDHGGQP
                     DKDGKRRIFSRVETMAPGTICTESYILVGPFATKTEADLMVKYLKLKLPRFLVSTILL
                     TQNIAKDKFAFVPLIDISQKTKDKDLYNMFGLTHKEIDYIESTIRPME"
     gene            complement(1937..2596)
                     /locus_tag="P5123_10285"
     CDS             complement(1937..2596)
                     /locus_tag="P5123_10285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013685369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SAM-dependent DNA methyltransferase"
                     /protein_id="HRX16690.1"
                     /translation="MSNQERQIISKKRVADHGEVYTSEREVKAMLDLVKSETERVDSR
                     FLEPACGNGNFLIEVLKRKLNVVKQKYNHSRIEYEVYALLTISSLYGIDILEDNVKAC
                     RERLYSFFSQEYGKLFKDKCEKKYYETVSFMLERNIIWGDALTLLRVDIPDEPIIFSE
                     WSLVNEVKFKRRDFTMSHLLECAPLEGKNLFSDLGEDVFIPTPIKEYPLMHYLELNNA
                     D"
     gene            2805..3539
                     /locus_tag="P5123_10290"
     CDS             2805..3539
                     /locus_tag="P5123_10290"
                     /inference="COORDINATES: protein motif:HMM:NF013793.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ParA family protein"
                     /protein_id="HRX16691.1"
                     /translation="MKSYAIHILKGGTGKTTTAGNVAFWLGQTSKTLLIDADMQGNSS
                     TWFCSNKNISVDLVDVLEDRCHVSDALVNIDSKFDMLPVFGSSKIKDFGETTLFKKPY
                     SFAELNEEISKLGYDYVVYDMSPSMSNLERCILLSVKEVITPITPEYFAIDGIELFTE
                     ELKAINKNWRRNILHDKVVVNNINRSINQHVVFSEQIKQMAYKVFEISQSSKIREAQL
                     NNQSIFEYYEKDRTIPQFISLAKAMR"
     gene            3551..3772
                     /locus_tag="P5123_10295"
     CDS             3551..3772
                     /locus_tag="P5123_10295"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRX16692.1"
                     /translation="MVLKSSDKNRKLMEEKVQKSGLKKKEKNEARIFMAISNTDKEII
                     ERYAAENGLSLAAAIRYILSQWMKDNNLK"
     gene            4741..5835
                     /locus_tag="P5123_10300"
     CDS             4741..5835
                     /locus_tag="P5123_10300"
                     /inference="COORDINATES: protein motif:HMM:NF013235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006270 - DNA replication initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="HRX16693.1"
                     /translation="MEMMTSGPDKKLEIVNAVLNKDILVLRKHDKLVRMPNPLTKLQH
                     KIYNIMLVKAQLITDIVDPTQKHFNIDIKTLANLSGMSAARNPTYLKETLSSLLQTDI
                     QIEQDDETYSGINLLGSYSVAKGVLNYNLTDDILKLVKEKERFANLVTQELKKLNNKY
                     SLILYEICINARHRNEIEISLDELKKIMQHPDYEYKYIKSKVINSAILEINTSTNIKV
                     DYKEIKKKGKVVGIKVEIKEIQKPLNSISDHKKENIIITLLEQLKIGHTKKDAKVLAA
                     NYKLENLTKAYHIYKKAKSEPLNPIAYFTNIVKNYEQYGYYTPEEQETLAAIEKDTVS
                     NFQKKLLESIDLETKGLLKGGVSRNPSEEQ"
     gene            complement(5958..6134)
                     /locus_tag="P5123_10305"
     CDS             complement(5958..6134)
                     /locus_tag="P5123_10305"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRX16694.1"
                     /translation="MDPKTIKIIKEWRENMEQAQAMEFALVAEEQYKNAPDEKKIIAE
                     MTAYWKDYFKNKKE"
BASE COUNT         2127 a         1192 c         1047 g         1987 t
ORIGIN      
        1 tattcgggtt ttcttttcta aaatccttaa tatagttgat tgttttttca atagcagtct
       61 tttgctctgg tctcaaagaa aaacttaaac tcctgtcgtc ttcaacatac cttctttctt
      121 taattccaat aatcgcattt ttaacatcgt ctacagaaca tttaaaccat tctccatttg
      181 ggttttcaaa ccctcttcgt tttaaatatt tgtggacatc atgatcagta aaagaagtcc
      241 catcgtttct aatagcaggc tccgataaaa gtattttgta gggtttttcg cctggcctta
      301 agattgggta ttgttgagat acgcgtgctt ttacatctat agtcgtataa ccaattttaa
      361 gaagtccatc gtattgagga ttggtgtcca tataggcata tattcttggg tctacttcag
      421 gtcgagaagg aaagtattta tctgccataa ttactccata ggtcgaatcg ttgattcgat
      481 ataatcaatc tctttatgag ttaaaccaaa catgttatat aaatctttat cttttgtctt
      541 ttgagaaata tcaataagag gcacaaacgc aaacttatct tttgcaatat tttgtgtcaa
      601 aagaattgtt gatacaagaa atcttggcaa ttttaatttc aaatatttta ccattaaatc
      661 cgcttctgtt ttggttgcaa aagggccaac taatatatat gattctgtac atattgtccc
      721 aggcgccatc gtttctactc ttgaaaatat tcttctcttc ccatccttgt caggttgccc
      781 accatgatca aatgatgctt ttgataaaag cactttgtat ttatcgatat aattgtgtcc
      841 agattttact ttttcccttg ggaaagggcc atcaccgcca ctccatatta gtgttaaatc
      901 gccattttta gttggtctat cttttgactc aagtccaaat ggttttctgc tcgacacaat
      961 ggaatctaaa tattctctac ttttttctct tattttatga attattgata cagctatacc
     1021 atctctaatg aatatatcaa attcatttaa cggcctaatc gttttagttg atgtgccttt
     1081 gttaaaatta attacagtac attttccatg ataatctttt tcccacaaga agtaacatac
     1141 accaccagca atatcaacac taggaaaaca atcacgcgaa tctgaataat ctataagata
     1201 tttgaccctt ttatcattca gcattttatt tcgaaaacag tctaatcctt ttccactaga
     1261 aaaccatctt gagggaataa tcatacaaag aaaactcggg tttaattcca gtgctttttc
     1321 aacgaaataa ttatatatcg gtcttgcact tgaaccagtc ccgccaccgt cagtcatctg
     1381 atacggcgga ttcccaacga taacatcgaa tttcatatta aatatctcct ctggtttata
     1441 tgtgtgaata aactgatatg catgagtttc aagttctggt actctatcat aaatgctttg
     1501 actagccccg caaaatgcac atctcccatt ttcccaacca tgttctgtct tgttgtataa
     1561 gatattcccc tgttcatcgt taaaagagct gcaaaccgaa taatctccat ttgccttttt
     1621 agaacaatac acagttcgcc gagataacag agccgtcaat tcggttatag caataccata
     1681 aacctggttt ttaaggatat ggtctgctct ttcctgaaca tcagggatct ttgtctttaa
     1741 cccctccatg agtctttgtg tgatttccct taagaagaca cctgttttcg tgaagggatc
     1801 cagaaattta atagtgctat catgccatat ctcatccggg agcaaatcta gcatttcatt
     1861 agccagcttg ggtggtgtga aaacttcgtc actactcaga ttagctatac aagacaatac
     1921 atccggatta tatgaatcag tcagcattgt taagctccag ataatgcatt aatgggtatt
     1981 ctttaatcgg ggttgggatg aaaacatctt ccccaagatc cgaaaaaaga tttttccctt
     2041 ccaaaggagc acattcaagt aagtgtgaca tcgtgaaatc cctccttttg aattttacct
     2101 cattcactaa agaccattcc gaaaatatga ttggttcatc aggtatatca actctaagca
     2161 atgttaaggc atctccccat ataatatttc tctcaagcat gaaacttact gtttcataat
     2221 attttttttc acatttgtct ttgaatagct tcccgtattc ttgagagaaa aaactataga
     2281 gtctttctct gcaagcctta acattatcct ctaatatgtc aatcccatat aaactggaaa
     2341 tcgttaataa cgcatatact tcatattcaa tgcgtgaatg attatatttt tgtttaacaa
     2401 catttagttt tcttttaaga acttctataa gaaaatttcc gtttccgcat gcaggctcta
     2461 gaaaacgaga atcaactcgc tctgtctcac ttttgacaag atctaacatt gcttttactt
     2521 ctctttctga agtatagacc tccccatgat cagcgactct tttttttgat atgatttgtc
     2581 tttcttgatt actcatttat ttccacccca acctatcgtt tagattatac agcaaccgta
     2641 gttttctgca actcttacaa taattttatt atataccctt gtaatgttaa ataaaggcat
     2701 tataatcata atatgaaaac aaaattcatt cggcattgga attatataaa actaatatcg
     2761 gaacaaaata catattgtat tctatacata taggaggtat ttgaatgaaa tcgtatgcga
     2821 tccatatcct taaaggcgga accgggaaaa ccacaaccgc cggtaatgtg gccttctggc
     2881 ttggacagac ctcaaagacc ttgctcatcg atgctgatat gcagggcaat agctcaacct
     2941 ggttttgcag taataagaat atctcggttg acctggttga tgtgttagag gatcggtgtc
     3001 atgtatcaga tgctctcgtc aatatcgaca gcaaattcga tatgctccct gttttcggaa
     3061 gcagcaaaat caaagatttc ggggaaacaa ctctgtttaa gaagccttac tctttcgcag
     3121 aactcaacga agaaatcagt aaacttggat atgattatgt tgtttatgac atgtcccctt
     3181 ccatgtcaaa ccttgagcga tgcattcttc tcagcgttaa agaagtaatc accccgatca
     3241 ccccggagta ctttgccatt gacggaatag aactgtttac agaagaactc aaggctatca
     3301 acaaaaactg gaggcgtaat atcctgcatg acaaagtcgt agtcaacaac atcaaccgct
     3361 ccatcaacca gcatgtcgtt ttctctgaac aaataaagca aatggcctac aaggtctttg
     3421 aaatatccca atcatcaaag atccgggaag ctcaactcaa caatcaatcc atttttgaat
     3481 actatgaaaa agaccgaacc attccccagt ttatatcact ggccaaagcg atgcgatagg
     3541 aggtattgaa atggttttaa agtcctcgga taaaaacagg aagctgatgg aggagaaggt
     3601 ccaaaaatca ggcctcaaga aaaaagaaaa aaacgaggcc cggattttta tggccatctc
     3661 taataccgat aaagagatca tcgaaagata tgccgctgag aacgggctga gcctggcggc
     3721 tgcgatccgg tatatactaa gccagtggat gaaagacaat aatttaaagt gaaaaacacc
     3781 ccttaaaagc gtttctcttc ttttggcgga aataatatca cccgaacctg acaaacccgt
     3841 ttaaaggaca atctgaagcc tcctaaggct atattatatc catggcttaa ccccggatca
     3901 gcagaagttt atagaaagtg accttttgtg aaaactgttt atagaaagtg accttttcta
     3961 taaaatttat aaaagcctat tttgatgtat aataactaga tagaagcttt taagaaatat
     4021 taaaaagaaa aataaataca tccttgcgta cgaattacgt agaattgcgt agaattgcgt
     4081 agaatgtagg cctcgtaact aacaaatata aagggtttta caacgacaaa ggtcaccttt
     4141 tataaacaca aaggtcacct tttataaaca caaaggtcac tttctataaa caaatacttc
     4201 tttttccggt tgtatttatg ttgtcaatag gagatcaacg aggttctgag tatattctga
     4261 gcttgataaa aaaagaaccc ttgcggagga gaaggtccaa aaatcaggcc tcaagaaaaa
     4321 agatgccagg atctttttgg ccatctcgaa tatcgaaaat atctactatg cataggaaaa
     4381 tttaccctat gaggataacg gactttgtct tttcccggat ttatagaaag tgaccttttg
     4441 tgtaaaatgt ttataaaagg tgactttttc aaaaaaatta tataactttc tatgttgtta
     4501 tatatcatat ggatataatt attatccaca taagagtcat ctatgtattt gtaagcaaga
     4561 attcaagaaa taagtatcta tatataaatc aaaaaaaagt aattactgaa tataataaca
     4621 gttagagaag ataggtcacc ttttataaac acaaaggtca ccttttataa atataaaggt
     4681 cacacaaaat gaaaaaatca cttgattaaa tttatcagat gagtatggtt gttgaatagt
     4741 atggaaatga tgacttcggg ccctgataaa aaactagaaa tagtcaatgc tgtactcaat
     4801 aaggatattt tggtactgcg taagcatgac aagcttgtga ggatgcctaa tccattaaca
     4861 aaattacagc ataagattta taatataatg ctggtgaaag cacaattaat cacagacatt
     4921 gttgatccca ctcaaaaaca ttttaacata gacattaaaa cattagctaa tttatcaggg
     4981 atgtctgcag cgagaaaccc gacatattta aaggaaacac taagttcgtt gttacaaacg
     5041 gatatacaaa tcgagcaaga cgatgaaacg tatagcggaa ttaatttatt aggttcttat
     5101 tcagtcgcta aaggtgtact aaattacaat ctcacagacg atatattaaa actagtaaag
     5161 gaaaaagaaa ggtttgcaaa cctggttaca caagaattga aaaaattgaa taataagtac
     5221 agtttgattt tgtatgaaat atgtatcaat gcacgtcaca gaaacgagat agaaataagt
     5281 ttagatgaat taaagaaaat aatgcagcac ccggattacg aatataaata cattaaatca
     5341 aaagtaataa actctgcaat ccttgagatc aatacatcaa caaacattaa agtggattat
     5401 aaggaaatca aaaaaaaagg caaagtggtt ggaataaaag tagaaatcaa agaaatacag
     5461 aagcctctta attcaatcag cgatcacaaa aaagaaaaca ttataattac tttgctcgaa
     5521 caattaaaaa tagggcacac aaaaaaagat gcgaaagttt tagcggccaa ttataaattg
     5581 gagaatttga caaaagccta ccatatatat aaaaaggcca aatcagagcc tcttaatccg
     5641 atagcttatt ttaccaacat tgtgaagaat tatgaacaat acggatatta tactcctgaa
     5701 gaacaggaaa cattagcggc catagagaag gacacagtat cgaattttca aaagaaacta
     5761 ttagaatcaa tagatttgga gacgaagggt ttattaaaag gcggggtgtc gagaaatcct
     5821 tcagaggaac agtgaataaa aaaacaagac atccgcttat cgtgcgtagg ttatgatcta
     5881 tttctccgat cctatattcc attttttttc acatactacc aacattcata ttttttataa
     5941 tttgagtgga gtttttatta ttctttcttg tttttaaaat agtctttcca atatgccgtc
     6001 atctcggcga tgatcttttt ctcatctggg gcgtttttgt attgttcttc ggccaccagg
     6061 gcgaactcca tagcctgggc ctgctccatg ttctcacgcc attctttaat gattttgata
     6121 gtcttgggat ccataaaata cctccttttc gaatgttttc tgcatttcat gtttgagtga
     6181 attcataagt tttatacgaa ccttttacag aaacggatac ttctcttgat tatatgatga
     6241 tacaaccatt tatcaaggcg ttttctcctg ataaaacaca aaacactcct gaaagaagtg
     6301 gggcctaagc caaaaggcac gacaccgctt aaaaagctcc agaaggcccc tta
//
LOCUS       DAOTGZ010000167         6311 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_3064
            NODE_14643_length_6311_cov_3.887628, whole genome shotgun sequence.
ACCESSION   DAOTGZ010000167 DAOTGZ010000000
VERSION     DAOTGZ010000167.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868088
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 6311)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6311)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:22:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,785
            CDSs (total)                      :: 2,751
            Genes (coding)                    :: 2,732
            CDSs (with protein)               :: 2,732
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 12 of 19
            Pseudo Genes (internal stop)      :: 5 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6311
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14643_length_6311_cov_3.887628"
                     /isolate="AMR_MDS_3064"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..688
                     /locus_tag="P5123_10310"
     CDS             <1..688
                     /locus_tag="P5123_10310"
                     /EC_number="5.4.99.-"
                     /inference="COORDINATES: protein motif:HMM:NF013045.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HRX16695.1"
                     /translation="LKHMLNGMMVDENPVIPLSVSDCTIEIIYEDDLIAVVNKPSGLL
                     TVPGKDNHDSLYSIMKKRYPGATGPLTVHRLDMSTSGLIIIAKDQQTAVNLQRQFMDR
                     KVKKRYIAIIEGTIDDDNGIIKLPLRGDPLNRPYQIVCFDYGKPSVTRYNKIDTNNGQ
                     TRVQLFPLTGRTHQLRVHLAHPQGLGCPIKGDELYGTPSDRLYLHADLIKFYHPASGK
                     KISFSCPPKF"
     gene            complement(696..2780)
                     /locus_tag="P5123_10315"
     CDS             complement(696..2780)
                     /locus_tag="P5123_10315"
                     /inference="COORDINATES: protein motif:HMM:NF014026.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 3 N-terminal
                     domain-containing protein"
                     /protein_id="HRX16696.1"
                     /translation="MNINSKDHFKPLLSHAQASAKAAEVVSRMTLEEKIEYIGGHNYF
                     FIKGCDDLNLPRLYLTDATQGIHIRENIEGQPDKSTAFPAPIALASSWNVDLAHEYAD
                     CIARECRASGIAVLLAPGMNIYRNSQNGRNFEYLGEDPFLAARFVEKYVTGIQNMGVM
                     ATLKHFVCNNSDFRRRRSNSIVNERTLFEIYLPAFKAGIDAGAMAVMTAYNQLNGEWT
                     GQSSYVIKDLLRNRLGFKWLVMSDWWSVWDPAKAIKSGLDIDMPGHGRGGEDDFEDFG
                     GPFLRSNAAKLVSEGEVVEGDIDRMVTNLFATSLAMGFDRPGFKDESFIKNMSVHVKT
                     ALQTAREGTVLLKNSNNVLPVVPQSDKTILLTGDFTTKNAFGGGAAEVEGYDVITLEA
                     AFKQVYGANLVCSVDPDASLIKSADVVFISVGTLDHEGWDSPFELDKSVNDRINNIAS
                     INKNSVVIVQSGRGVNMSEWIDNIAGLLYGWYPGQIGNLAVAEIASGMVNPSGKLPIT
                     IERSFKDSPAYPYIPEGEGFYEGWDVDNDLTYPINDIHYNEGVFTGYRWYQKMAIKPL
                     FAFGFGLSYTNFLYSDIKADKVSAYIGSEIIVSFKITNTGVRDGSEICQLYVRDNESS
                     VERPYMELKGFTKLFVPAGEAVNGSIKLDAASFAFYDEAIHDWKIEPGDFTLYLCSSS
                     DKIECETVVTLK"
     gene            complement(3054..3857)
                     /locus_tag="P5123_10320"
     CDS             complement(3054..3857)
                     /locus_tag="P5123_10320"
                     /inference="COORDINATES: protein motif:HMM:NF016915.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRX16697.1"
                     /translation="MKKRCDTKYPILLVHGAGFRDDSRFYNYWGRIPKALTDEGATIF
                     YSGQDAWGSIEYNGTIIKERIEQVLKITKAGKVNIIAHSRGGLEARYAITKLAMGKYV
                     ASLTTISTPHYGSKTMNIFYKFPKGLYRFVSSIVNLYFKVLGDKNPDFFTSSRQFSTY
                     EAEIFNKNTPNDSSVLYQSYAAKMKKSLSDPLFVLTHLIISFFDGENDGLCPVASAKW
                     GNYRGLITGKNFWGISHAGVIDSYRFNYSGIDLPEFYVNIVEGVKKENL"
     gene            complement(3971..5359)
                     /locus_tag="P5123_10325"
     CDS             complement(3971..5359)
                     /locus_tag="P5123_10325"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase dimerization/phosphoacceptor
                     domain -containing protein"
                     /protein_id="HRX16698.1"
                     /translation="MKKNDNKIESSVTDRSLLNINAFGVLLVSSHGVILDCNRRFSEM
                     INIDIDKLKGKSVSILPYDPQDLKSSLVIYNKIAYGEEETTIRKFILDGNKTIIVSMN
                     TFMNVDGSFYSLFHDITEKKIIEDNLKMKSMFLEAVANSSIDGILVVDLKGKKILQNQ
                     RTLDLWQISPEIAADEDGSKQVDHVLKMTKNPEKFYEEIEYQKKHPYKTTLDELELVN
                     GTVLHRHSAPVLGEDGKNYGRIYHFHDITSFKKAEDKIRSLLEEKEIILKEVHHRVKN
                     YMSTLIGLLVLQTSTLKDDAAIHALDDAKQRLSGMMTLYEKLYVSGSGGSISTKEYLP
                     DLIDEIIANFPESEIVTVNKNIDDIILDAKTLQTISIITNELLTNIMKYAFKGRSSGT
                     IEIKFKVNNHKIIFAISDDGVGINEKILKTKIGGFGLMLVKSLVSNLKGDIDFRYDNG
                     TEVLISFDIINR"
     gene            5791..>6311
                     /locus_tag="P5123_10330"
     CDS             5791..>6311
                     /locus_tag="P5123_10330"
                     /inference="COORDINATES: protein motif:HMM:NF012655.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HRX16699.1"
                     /translation="MKKVNKADKIELKKAEILDALKRCLLKDVYSQISFNDVADEAGM
                     SKGGVRHYFPTKESLFMGLIEDFFNTIEEEQINVIKAATKMSDKALLSTMYSIEKFLM
                     NQDNVIIMMNIIIYSFEDNKIKELVRSFLRHHLQLFENLMKDLANDTNIDQNQVRQIS
                     RTIQVILLSTGII"
BASE COUNT         1971 a         1298 c         1057 g         1985 t
ORIGIN      
        1 acttaaacat atgcttaatg gaatgatggt tgatgaaaat cctgttatac ctttatcagt
       61 ttcagattgc acaatagaaa ttatttacga ggacgatctg atcgctgtgg ttaacaaacc
      121 atcaggactt ttgactgtac caggaaaaga taaccacgat tctctctatt caataatgaa
      181 gaagagatac cccggagcga ctggcccact aacggtacac agactcgata tgtcaacatc
      241 gggattaata ataatagcga aagaccagca aacagctgtt aatcttcaaa ggcagtttat
      301 ggacagaaaa gttaaaaagc gttatatcgc aattatcgaa ggcactatag atgatgataa
      361 tggtatcata aaactaccgc ttagagggga tccgctaaac agaccgtatc agattgtctg
      421 ttttgattat ggcaaaccta gcgttacacg ctacaacaag attgatacaa acaatggtca
      481 aacaagagtc caactctttc cactaactgg gcgcacacac cagcttagag ttcatcttgc
      541 tcatccccaa gggcttggct gccccataaa gggagacgaa ctgtacggaa caccttctga
      601 caggctctat cttcacgcgg atttaataaa attttatcat ccagcaagcg gaaaaaaaat
      661 cagttttagc tgtccaccaa aattttaata tgtttttatt ttaaagtaac tacagtctcg
      721 cactctattt tatctgacga cgaacagagg taaagagtaa aatcgccagg ttcaattttc
      781 cagtcatgaa tcgcttcgtc gtaaaacgcg aaagatgccg catcaagctt tattgatccg
      841 tttaccgctt caccagcagg tacaaagagt tttgtaaaac ccttaagctc catatatgga
      901 cgttcaacag aggattcatt gtcacgaaca tagagttgac agatttcact tccatcacga
      961 acacctgtat ttgtaatctt aaacgaaacg attatttcgc taccgatata agcgctgacc
     1021 ttgtcagcct ttatatccga atagagaaaa tttgtatatg acagaccaaa gccaaaagca
     1081 aacagtggtt tgatagccat tttctgatac cagcgataac ctgtaaatac accctcgttg
     1141 taatgaatat cattaatcgg ataggtcagg tcattgtcca cgtcccaacc ctcgtaaaaa
     1201 ccttcacctt cgggtatata tggataggcc ggtgagtctt tgaacgatct ctcgatagtt
     1261 atgggcaact tacccgacgg attaaccatt ccactcgcta tttcggccac agccagatta
     1321 ccaatctgtc ccggatacca gccatacaag agtcccgcta tgttatcgat ccactccgac
     1381 atattaacac cacgaccgct ttgcacaatt acaaccgagt ttttatttat tgacgcgata
     1441 ttgtttatac gatcgttcac gcttttatcg agctcaaatg ggctatccca cccctcatgg
     1501 tcaagggttc ctacactgat aaaaacaacg tcagctgatt ttatcagtga cgcatcagga
     1561 tcaacactgc atacaagatt ggcaccatac acctgtttaa acgccgcctc taaagttatt
     1621 acatcgtagc cttctacctc ggcagcacca ccgccaaagg catttttagt agtaaaatct
     1681 cctgtcaaaa gaatcgtctt gtcactttgt ggcacaactg gaagtacgtt attgctgttt
     1741 ttaagcagta cagtgccctc tcttgcggtc tgaagcgctg ttttcacatg gactgacata
     1801 tttttaatga agctttcgtc tttaaagcca ggtctgtcaa accccattgc gagcgatgtt
     1861 gcaaagagat ttgttaccat acgatcgata tcaccctcaa caacctcacc ttcgctcaca
     1921 agctttgcag cgttacttct taaaaacgga ccgccaaaat cttcaaaatc atcttcgccg
     1981 ccgcggccat gccccggcat atctatatca agccccgatt taatcgcttt tgcgggatcc
     2041 cataccgacc accagtcgct cataacaagc cacttaaagc ccagcctgtt acgtaaaaga
     2101 tcttttataa cataagagct ctgccctgtc cactcgccat taagctgatt gtaagccgtc
     2161 ataacagcca tagcacctgc gtctatacca gctttaaacg caggaaggta aatttcgaaa
     2221 agggttcttt cgttaacaat ggagttcgag cgacgtcttc taaaatcgct gttgttgcat
     2281 acaaaatgct taagcgttgc catcaccccc atattttgta tacctgttac gtacttttca
     2341 acaaatctag ccgctaaaaa gggatcctca ccaagatatt cgaagtttcg gccattttgg
     2401 gaatttcggt aaatattcat tcccggtgca agtaaaacgg cgattccgct tgccctgcac
     2461 tctctggcaa tacagtctgc atattcatga gctagatcta cgttccaact tgaagcaagt
     2521 gcaatcggag caggaaaagc agtcgactta tcgggctgcc cctcaatatt ctcacgaata
     2581 tgtattccct gagtcgcgtc agtaagatag agtcgtggaa ggttaagatc atcacatccc
     2641 ttaataaaaa aatagttatg tccgccaata tactcgattt tttcttcgag agtcattctg
     2701 cttacaactt ctgcagcctt tgcgctggcc tgagcatgag acaatagcgg cttaaaatga
     2761 tctttactgt ttatattcat gatccttccg ttgatgtttg aaatcgaaca tttacagtgg
     2821 tttctgataa ttactgctat aatattagtt tgcagaattt agaaagacca catgttacat
     2881 gtgttatata accaatgtaa gcattgttga aaaattgaca ataatatttt ttaaaattga
     2941 gattattttg gatagaaagt taaactaaga gtaagtaaat ttacacctta ttctttccat
     3001 ttttaaagag aagaaggcgt tatgatgata attactatat tttttgacaa aagttacaga
     3061 ttttcctttt ttacgccttc aacaatattg acataaaatt cagggagatc aatacctgag
     3121 taattgaagc gataactatc tatgacaccc gcatgggaaa tgccccaaaa atttttgcct
     3181 gttattaatc ccctgtaatt gccccacttg gccgacgcaa cggggcaaag accgtcattt
     3241 tctccatcaa aaaatgatat aataaggtgt gttaaaacaa acagtggatc actcaaagat
     3301 tttttcattt ttgccgcata actttggtat aacacactgg aatcattcgg agtattttta
     3361 ttaaatattt cggcctcata tgtcgagaac tggcggctgc ttgtaaagaa atccggattt
     3421 ttatcaccca aaactttgaa ataaagattc acaatagatg atacaaatct gtacaaacct
     3481 tttggaaact tataaaagat attcattgtt tttgaaccgt agtgcggtgt cgagatagtt
     3541 gtcaaagagg caacatattt acccatagct aattttgtaa tagcatacct cgcttccaga
     3601 ccaccacggg aatgggcaat aatattaacc ttaccagcct ttgttatttt tagaacttgt
     3661 tcgattctct cttttattat agtaccatta tattctatag aaccccaagc gtcttgccca
     3721 ctataaaaaa ttgtagcacc ttcatcagtg agagccttag gtatgcgtcc ccaataattg
     3781 tagaaccgag aatcgtcacg aaaacccgct ccatgaacca aaagaattgg atacttggtg
     3841 tcgcatcttt ttttcatatg ttattccagt atatttagtt ttcaaatggt tcgttaccaa
     3901 aacaccttta gcaagctgat tgaaatcata gattatagtt ttaatattta cagggaagaa
     3961 atttatttaa ttatctattt attatgtcaa atgatattaa tacttcagtt ccattatcat
     4021 atctaaaatc gatgtcacct tttagattgc taacaaggct ttttacaagc atcagcccga
     4081 acccgccaat tttagttttt agtatttttt cgttaatccc taccccatcg tcacttatag
     4141 caaaaattat tttatgatta ttaaccttaa attttatttc tatagtaccg gatgagcgac
     4201 ctttgaaagc gtatttcatt atatttgtta aaagctcatt agtaataata ctgatggttt
     4261 gcagggtttt agcatcaaga attatatcat caatattttt attaactgtt acaatttcac
     4321 tttcaggaaa attagcaatt atctcatcta ttaaatctgg taaatactct tttgtagaaa
     4381 tagagccgcc gctaccggat acatataatt tctcataaag cgtcatcatg cccgaaagcc
     4441 tttgttttgc atcgtcaaga gcatgaattg cagcatcgtc ttttaatgtg gaagtttgta
     4501 ataccagtaa cccgatcaga gtactcatat agttcttaac gcggtggtgc acctctttta
     4561 aaattatctc tttttcttca agaagcgacc ttattttatc ttctgctttt ttaaaactgg
     4621 taatgtcatg aaagtgataa atccgcccat aattttttcc atcttcacca agaaccggag
     4681 cggaatgccg gtgaagcact gttccattaa ccagttcaag ttcatcaagg gtggttttgt
     4741 aaggatgctt cttttggtac tcaatttctt catagaattt ttcggggttc tttgtcattt
     4801 tcaaaacatg atcgacctgc ttgctaccgt cttcatcagc agctatttca ggtgatattt
     4861 gccaaagatc aagagttctt tgattttgaa gaattttttt acctttcaag tcaacaacaa
     4921 ggattccgtc tatagaggag tttgctacag cttcgagaaa catggatttc attttcaaat
     4981 tatcttcaat tatctttttt tctgttatat catggaacag agaatagaaa gagccatcaa
     5041 cattcataaa tgtgttcatg gaaacaataa ttgttttgtt accatccaga ataaatttac
     5101 gtatcgttgt ctcttcttcg ccgtacgcta ttttgttata gataaccaga cttgatttaa
     5161 gatcctgagg gtcatacggc aaaatactga cagatttacc cttaagttta tcaatgtcaa
     5221 tatttatcat ttcggaaaaa cgccgattgc aatcaagaat aaccccatga gatgatacaa
     5281 ggagaacacc aaatgcatta atgtttaata aggagcggtc tgttactgag ctttcaattt
     5341 tgttatcgtt ctttttcatt tgccctctaa atgattaaat gacaaaaata taaacaaaga
     5401 tgcttatgct aatagcaata gtcatccata acatcaaaat tattaaaaaa agcaactcta
     5461 ctgttattga agatatgtca caaaaaaata atgtcaatat tttgggacaa tcataatgca
     5521 tttctatttt tttgctacaa ttttgtaata ttaccatttt cattaaattc atggctaata
     5581 ttcttataaa cttttgactc attttgttta cataattctt aagccagtgt gtcgtaccac
     5641 tatattttgt aaatcccaag accgaaaccg taggccattt attgttcgtt gaccaagcct
     5701 attttaaccc tatttttttt acaaaaaccc tttacttttt tattatatct atttagtttg
     5761 agtaaaatgt aaaaaaatac cggtatatat atgaaaaaag ttaataaggc tgataaaatt
     5821 gaactaaaga aagccgaaat tcttgatgct ctaaaaagat gccttttaaa ggatgtctat
     5881 tcacagatat cttttaatga tgtagcagat gaagcaggaa tgtcaaaagg tggtgttcgc
     5941 cactattttc caaccaaaga gagtctcttt atggggctaa ttgaagactt ttttaataca
     6001 atcgaagaag agcagataaa cgtaatcaag gccgctacca agatgagcga caaggctctt
     6061 ctctctacaa tgtactctat tgagaaattc cttatgaatc aggataatgt cattataatg
     6121 atgaacatta tcatctatag ctttgaagac aataaaataa aagagcttgt tcgctccttt
     6181 ttacgtcacc atcttcagtt gttcgaaaac cttatgaaag accttgccaa cgacacaaat
     6241 atcgatcaaa accaggttcg ccaaatcagt cgtactatcc aggttattct tctttccaca
     6301 ggaatcatac a
//
LOCUS       DAOTGZ010000168         6303 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_3064
            NODE_14668_length_6303_cov_4.263284, whole genome shotgun sequence.
ACCESSION   DAOTGZ010000168 DAOTGZ010000000
VERSION     DAOTGZ010000168.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868088
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 6303)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6303)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:22:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,785
            CDSs (total)                      :: 2,751
            Genes (coding)                    :: 2,732
            CDSs (with protein)               :: 2,732
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 12 of 19
            Pseudo Genes (internal stop)      :: 5 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6303
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14668_length_6303_cov_4.263284"
                     /isolate="AMR_MDS_3064"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..769)
                     /locus_tag="P5123_10335"
     CDS             complement(<1..769)
                     /locus_tag="P5123_10335"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HRX16700.1"
                     /translation="MKRILIFIFLTMNITLIASEYSTGVRLFMRKNPDKAKEHFLAAV
                     EEDSTNGNAYYYLGEIEKNNRNYKQAIEYYIKALDNRLSGKYQKLTHWNLIILTEQIN
                     DLDGMVKTCKIMYEKRGDHAAKKKVDELINKMLWTNNQSAIDAYKSGKSNHSSGNTQN
                     ALDDFKSALSYDNSFLAPRFEIGIINYKSGNVGEALRNLRVIANKIPYYTAVQTLAAK
                     ISMDNKQYSDATTYYSNILKYGFINTNTRLNTLSQRAS"
     gene            953..2515
                     /gene="murJ"
                     /locus_tag="P5123_10340"
     CDS             953..2515
                     /gene="murJ"
                     /locus_tag="P5123_10340"
                     /inference="COORDINATES: protein motif:HMM:TIGR01695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0034204 - lipid translocation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="murein biosynthesis integral membrane protein
                     MurJ"
                     /protein_id="HRX16701.1"
                     /translation="MKKQVIKSTVVVAGSTMLSRILGFFRDAVMANYFGTGASFDGFI
                     VAFRIPNLLRRFVAEGTLTICFVPVYTEYLEIHGKDAALKLAQKTLSFLIVILTVLTL
                     SGIVFSPRLVALFGYGFTDQTLISSTISLNRIMFPYIFMVSLVAFCMGYLNSHRSFFA
                     PAVSPVLLNLGMIFGALVLRNYFENGLWGLAFGVLIGGTLQVVLQLPFMIRNGFRMRI
                     SFDYKHPGIRKIGFMILPAMMSGAVSQLNLLINTVLASMLPGGSVSYLYYADRLTEIV
                     VGVFVVSIAGVALPELSRYASRKDMVNLRSAFARAVTSSLFFSVPASVALMVSGHQII
                     EVLFMRGEFDMVSVSNTYKALFFASAGLFTLTFYKLLVPVFYSLQDTRTPFITAAVAL
                     LINAVTGYTLMQTSLAHAGLALSSVVSIMVQVVLLLVLLRKKMGRIGFRGIIKPAIKL
                     LFASAVMGVWLWFAQGRLVWGEDIVTTRKIAQLVFLIASGGILYITICYIIRVDELKT
                     VLSIIRRKMKRV"
     gene            2518..3762
                     /locus_tag="P5123_10345"
     CDS             2518..3762
                     /locus_tag="P5123_10345"
                     /inference="COORDINATES: protein motif:HMM:NF013697.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HRX16702.1"
                     /translation="MKKVFLVLLPFLFLVCSSDATDELTIAVPFGKEGELLIEQLDQF
                     CHEKNIKNITYTKLPISLNEFDDEFINSYLKTSICADVIPIKNCWTAPLTGMLEILDT
                     YQLDYELDMHLDSYVRNNYYYDNTLYSKLVALPYSADPVVLYINKALSEKYGFDKAIS
                     FDQLEKQCLEVIQKEKNIGNSVEGFVFCYKPNGKLFETVCDMLRLSDGGAVVNESYQS
                     TIGNSAAQNLFTSISRWIGTITPEIEENGKINILDMIGKNEALYVFAPASFSLSDNKK
                     FFAKYRLSAFGYEGGSKLTARGYSYGIKRGCQRMNDALKIIRFISSTKQQMLRYNKGL
                     AFPTYKEFYAAYEQNNKFLEGILSDIYMSESCLSKDLVSNYFYVKELFIDAIMPCITN
                     GQCTDVSNSFEYELLHLYNKAF"
     gene            4071..4613
                     /locus_tag="P5123_10350"
     CDS             4071..4613
                     /locus_tag="P5123_10350"
                     /inference="COORDINATES: protein motif:HMM:NF014669.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin transporter BioY"
                     /protein_id="HRX16703.1"
                     /translation="MNEDNIRKMTLSAVFTVLIIAGSYLVVPIGVVPIVLQNMFVLMA
                     GVMLGPLWGAVSVCAFLVLGALGLPVFSGGRGGAAHILGPTGGYLVAYIPAVIICGVI
                     NLKKSKKIILILFSMVSASLVIYLIGVTWLKLTTSISWDKAVFVGMLPFLLGDALKIA
                     VAAFMTFKLSPLLKNLGIGN"
     gene            4648..5394
                     /locus_tag="P5123_10355"
     CDS             4648..5394
                     /locus_tag="P5123_10355"
                     /inference="COORDINATES: protein motif:HMM:NF014516.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HRX16704.1"
                     /translation="MNLEVTNLCFGFEKNNYLLKNITFSVRTGEFIVLAGENGSGKSL
                     LMKHLNGLHAPSSGALLFNGKEVSKNKSEVRRKVGLVFQNSDAQFVAQTVYDEMAFGP
                     ENLGWEKNEIERKVSHYADIFGITPYLDRHPHFLSGGEKKRIAIAAVMIMRPDLVIFD
                     EPFATLDYKGVISVCNTIVDIYNQGCSVIVITHDLEKILAHATRLMIMKSGMLVYDGK
                     PSLEKRLLLDNNIRVPHGNRRSVESCTWLA"
     gene            5382..6146
                     /locus_tag="P5123_10360"
     CDS             5382..6146
                     /locus_tag="P5123_10360"
                     /inference="COORDINATES: protein motif:HMM:NF014420.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor transporter transmembrane
                     component T"
                     /protein_id="HRX16705.1"
                     /translation="MAGLNIFHYTSGSSILHRHNTLLFFLEFLLLSLVLFNTDGPIIM
                     LFIPFVVFLVILSGIDYASLIRQLKGFIFLGIIIGAAGYYRLEPASVTERLLSGLFDM
                     SRYFVFLLYSIVFIYTRSPRQIYIMIAGLFKFIPFISGRKIASFFLIVFSLLPVVFDT
                     IKDVRESIVSRCGYRGRRVFINTFYTVYPVVVTSLIRSANFADALISRSFTADNTLRQ
                     YEKITRTDVIIFIIITMLSVLVFFVQYSNFFDYSQI"
BASE COUNT         1712 a          976 c         1327 g         2288 t
ORIGIN      
        1 agctggctct ttgtgacaag gtatttagcc gggtatttgt attaataaag ccgtatttga
       61 gtatatttga atagtaagtt gtggcatcag aatactgttt attgtccatg ctaattttcg
      121 cagctagggt ctgaacagca gtatagtagg gtattttatt ggcgattaca cgcaggtttc
      181 ttaatgcttc accaacgttt ccacttttat aatttataat acctatttcg aatctagggg
      241 ccagaaaaga gttgtcgtaa gagagcgcgc ttttgaagtc atccaaagca ttttgagtat
      301 ttccggatga atgattgctt ttacctgatt tataagcatc tatagctgat tgattgttgg
      361 tccaaagcat cttgtttata agttcatcga cctttttctt ggcggcatga tctcctcttt
      421 tttcgtacat aattttgcat gttttaacca ttccgtccag atcgtttatc tgttctgtca
      481 gtattatgag gttccagtgg gtcagcttct gatattttcc gctaagccgg ttatcgaggg
      541 ctttaatata atattctatg gcctgcttgt agttacggtt gtttttttct atttcaccaa
      601 ggtaataata ggcatttccg ttggttgagt cttcttcaac agcagcaaga aagtgctctt
      661 ttgccttatc cgggtttttg cgcataaaaa gacgaacacc tgttgaatat tcacttgcta
      721 tcagggttat attcatagtt aaaaatataa agattaaaat tcgtttcata attgattaag
      781 cctggtatcc ccaaaaatat ctatataagc acggtaaatc tatttgttaa atgtagatca
      841 ttgagctgat tattgtcaat cggtaactta aaaaaaggat aatattttag cggtgtaaag
      901 tatttgaaaa aaaaacgact gcatataatc tgccatttga ctagggggat ttatgaaaaa
      961 gcaggtaatt aagtcgacgg ttgtggtagc cggctcgact atgctaagcc gcatattggg
     1021 gtttttccgt gatgctgtta tggcaaatta ttttggaacg ggagcctctt ttgacggctt
     1081 tattgttgca tttcggattc ctaatctttt gaggcgtttt gttgcagagg gaacactcac
     1141 gatctgcttt gttcctgttt ataccgaata tttggagatt catggtaaag atgctgcttt
     1201 gaaactcgca caaaagaccc tctcttttct tattgtcatt ctgacggttt tgactttgtc
     1261 ggggattgtg ttttcaccac ggcttgtagc acttttcggt tatggtttta cagatcagac
     1321 tttaatttcg agtacaatca gtttaaaccg aataatgttt ccatatattt tcatggtatc
     1381 actagttgct ttttgtatgg gatatcttaa ctctcatcgt agttttttcg cacctgctgt
     1441 ttcccccgtt cttttgaatc ttggcatgat ttttggtgcc cttgtattgc ggaactattt
     1501 tgaaaacgga ttatgggggc ttgcttttgg tgttttgata ggtggcaccc tgcaggttgt
     1561 tctccagctt ccttttatga tacgtaatgg ttttcgcatg cggatatctt ttgattataa
     1621 gcatcctgga attcgtaaaa tagggtttat gatattgcct gccatgatga gcggagctgt
     1681 aagccagttg aatctgctta ttaatactgt gttggcatca atgctacctg gcggtagtgt
     1741 ctcttatctc tattatgccg atcggttaac tgaaatagtt gttggagttt ttgtggtgtc
     1801 aatcgcggga gtagctttgc ccgagctgtc tcgttatgct tcacggaaag atatggtgaa
     1861 tttgcgttca gcgtttgcac gagcagtaac ctcttcgctc tttttttcgg taccggcgtc
     1921 agtcgctttg atggttagcg gtcatcagat aatcgaggta ttgtttatga gaggagagtt
     1981 tgatatggta tctgtgtcta atacttataa agctcttttc tttgcaagtg ccgggctatt
     2041 taccctaact ttttataaac ttttagttcc agttttttac tctcttcagg ataccagaac
     2101 tccttttatt acagcggcag tggcccttct gattaatgcg gtaaccggat atactttgat
     2161 gcaaacatcg cttgcgcatg caggtttagc tctttcgagc gtggtttcaa ttatggttca
     2221 agttgtgctt ctgcttgtgc tattgcgaaa aaaaatgggg cgaatcggtt ttagaggcat
     2281 tattaagccc gctataaaat tattatttgc ttctgcagtg atgggcgtgt ggctttggtt
     2341 tgctcagggg cgtttggtat ggggagaaga tatcgttaca acaagaaaaa ttgctcaact
     2401 tgtgtttttg atagcatcag gtggaatttt atatataact atatgttata taattcgcgt
     2461 tgatgagcta aaaactgttt tgagcattat cagacgcaag atgaagaggg tgtagatttg
     2521 aaaaaagttt ttcttgtttt gttaccgttt ctgtttttgg tatgctcatc tgatgctaca
     2581 gatgagctga caattgctgt gccttttggt aaagagggtg aattgcttat agagcagtta
     2641 gatcaatttt gtcatgaaaa gaacattaaa aatattactt ataccaaact cccgatatcg
     2701 ttaaacgagt ttgatgatga atttattaat agttatttga aaacatcaat ctgtgccgat
     2761 gttattccta ttaaaaattg ttggacggcg cctttaacgg gtatgcttga aatactggat
     2821 acttatcaat tggattatga gctggacatg catcttgatt cgtatgtccg taataactat
     2881 tattacgata atactcttta tagcaagctg gtggctcttc cttatagtgc tgatcctgtt
     2941 gttttgtata taaataaagc tttatccgaa aaatatggtt ttgataaggc tattagtttt
     3001 gatcaacttg aaaagcaatg tcttgaagta attcaaaaag agaaaaacat tggaaacagt
     3061 gttgaagggt ttgttttttg ttacaaacct aatggaaagt tattcgaaac tgtttgtgat
     3121 atgttgcgat tatcggatgg tggggcagtt gtgaatgagt cataccaatc tacaattggc
     3181 aatagcgctg cacagaatct ttttacaagt atttcaagat ggataggtac aataacacca
     3241 gagattgaag aaaacggcaa gataaatata ttggatatga taggtaaaaa tgaagctctt
     3301 tatgtttttg cacccgcttc attttcatta tctgataaca aaaaattttt tgcaaaatac
     3361 agattatccg cttttggata tgaagggggt tctaagctta cggcaagggg ttattcgtat
     3421 gggatcaaaa gaggttgtca acgtatgaat gatgctttga aaataatacg ctttattagt
     3481 tctacaaaac agcaaatgtt aagatataat aaaggtttag cttttccgac atacaaggaa
     3541 ttttatgcag cgtatgagca aaacaataag tttttggaag gtattttgtc ggacatttac
     3601 atgtctgaat catgtctcag taaggatctg gtatcgaact atttttatgt taaggagctg
     3661 tttatcgatg caataatgcc gtgcataacg aacggtcaat gcactgatgt ttctaactct
     3721 tttgaatacg agcttttaca tttgtataac aaggcgtttt agcgtataac atttttataa
     3781 gggctacact attgcttgtt ttaattaaaa ctctttaatc aaaaatatga agtgtcataa
     3841 tgttgacatt ttactgctat tgttgagttt tgatattagt gaacttcgaa aaatttccat
     3901 gttccagagg gtaacatggt ttaagtgcct attctaaagg aatttttcga agttcctttg
     3961 ctggtcgtcc agcactattt tctttaaaaa tggtgctgga ctgcttgcat atgcaaccta
     4021 gttgaaaagt taatttttag aggatgccat tatttagatg gaggaatgat atgaatgaag
     4081 acaatataag aaagatgact ctgtcagctg tttttactgt tttaataatt gcaggctcgt
     4141 atcttgtggt gccaattggt gtggttccaa tagtcttgca gaatatgttt gtgctgatgg
     4201 ccggagtaat gttgggtcct ttatggggtg cggtttcagt atgtgcgttt ttagttttag
     4261 gtgctcttgg acttcctgta tttagtggag gcaggggagg tgccgctcac atattgggac
     4321 cgacaggtgg ttatcttgta gcttatattc ctgctgtaat aatatgtgga gtgataaact
     4381 taaaaaaaag taaaaaaatt atattgatac tattttccat ggtatcggct tcacttgtaa
     4441 tttatttgat tggtgtcact tggcttaaac ttacaacatc tatttcttgg gataaggccg
     4501 tatttgttgg tatgttgcct tttttgctgg gtgatgcgtt aaagattgcg gttgccgcat
     4561 ttatgacttt taaactttca cctttattaa aaaatcttgg aataggcaac tgataagtta
     4621 tctgactcta aaaatgaaga gtgtcttatg aatcttgaag taactaatct ttgtttcggt
     4681 tttgaaaaga ataattacct gttaaagaat attaccttta gtgttaggac aggcgagttt
     4741 attgtgcttg ccggggaaaa tggctcaggt aaatctctgt taatgaaaca tctaaatgga
     4801 ctgcatgctc caagtagtgg agcactttta tttaatggca aagaagtttc caaaaacaag
     4861 agtgaagtca gacggaaagt tggactggtt tttcaaaatt cggatgccca gtttgtagct
     4921 cagacggttt atgatgaaat ggctttcggt ccagaaaatc ttggttggga aaaaaatgaa
     4981 attgagcgaa aggtttcaca ttacgcagat atttttggca ttacacctta tctggatagg
     5041 cacccgcatt ttttatcagg tggtgaaaag aagaggatag ccatagcagc tgtaatgatt
     5101 atgcgtccgg atctggttat ctttgatgag cctttcgcca ctctggatta taaaggcgtt
     5161 atatctgttt gcaatactat tgtcgatatc tataatcagg gatgctcggt tatagttatt
     5221 acacatgatt tggaaaaaat actcgctcac gcaacacggc ttatgataat gaagtcgggt
     5281 atgcttgttt atgatggcaa gccttctttg gagaaaagac tattattaga taacaatatc
     5341 agggttcctc atggaaaccg gcgaagtgtt gagagctgca catggctggc ctgaatattt
     5401 tccattatac ttccggtagt agtattttgc atcgtcacaa tacacttctt ttttttctgg
     5461 aatttctgct tttgagtctg gttttattta atactgatgg tccgataatc atgttattta
     5521 ttcctttcgt ggtttttctt gtgattcttt ccggaataga ttacgcatca ttgattcgcc
     5581 agttgaaagg ctttattttt ctagggataa taattggtgc agccggttat tatcggttgg
     5641 aaccggcctc ggtcacggaa aggttgttaa gtggattatt tgacatgtca cgctattttg
     5701 tttttctgct atacagcata gtatttattt atactcgttc tccaaggcag atatatatta
     5761 tgattgccgg actttttaag tttatacctt ttatatccgg ccgaaaaatt gcatcgtttt
     5821 ttttaatagt gttttcactt cttccagttg ttttcgatac aatcaaagat gttcgtgaaa
     5881 gtattgtatc ccgatgtggt tatagaggaa gacgcgtatt tattaatact ttttatacag
     5941 tataccctgt tgtagtcact tcgcttattc ggtcagcaaa ttttgcggat gcacttatat
     6001 cacgttcttt taccgctgat aatacgttaa ggcagtatga aaaaatcaca agaaccgatg
     6061 taataatttt tataatcatc acgatgctat ctgttttggt tttttttgtt caatactcca
     6121 atttttttga ttattcacaa atttaaactt aaattataaa tagtgatttc ataaaattat
     6181 tattgtagta ggggataaca tatttttagt gcttactata aatgaattaa tagcaagtgc
     6241 ctttttataa attcaattgt tacagatata aaatgtttat ttaagaatag gtgcggtttt
     6301 ctt
//
LOCUS       DAOTGZ010000169         6183 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_3064
            NODE_15050_length_6183_cov_4.684726, whole genome shotgun sequence.
ACCESSION   DAOTGZ010000169 DAOTGZ010000000
VERSION     DAOTGZ010000169.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868088
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 6183)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6183)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:22:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,785
            CDSs (total)                      :: 2,751
            Genes (coding)                    :: 2,732
            CDSs (with protein)               :: 2,732
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 12 of 19
            Pseudo Genes (internal stop)      :: 5 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6183
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15050_length_6183_cov_4.684726"
                     /isolate="AMR_MDS_3064"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..516)
                     /gene="bamD"
                     /locus_tag="P5123_10365"
     CDS             complement(<1..516)
                     /gene="bamD"
                     /locus_tag="P5123_10365"
                     /inference="COORDINATES: protein motif:HMM:NF024915.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamD"
                     /protein_id="HRX16706.1"
                     /translation="MKNFLNCTISYLLKSKWSCPILLTICIIVISTPAFPKSSEAIIR
                     IIAEPQNSSTDEQTLQPSIKPVQQNETASDQSQLVDNTSGQISSTKSDLAGNRSQMDI
                     PAPLSGDTVILESGINAYNSGDYNMAIEKFEELESLFQSSPFIMQAKLYLARSFYKTN
                     NREKALENIRSI"
     gene            complement(513..1175)
                     /locus_tag="P5123_10370"
     CDS             complement(513..1175)
                     /locus_tag="P5123_10370"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRX16707.1"
                     /translation="MKIVYTILASFNRFIEKMPVRQKQTIRQLFGMIIFVSVIAGVVA
                     GISMGKKAADKGGVPLVNTTRDVFEIDISRDKERASFADMIESEQLIEKDTESNEVRV
                     FPETEDTPLTSHKDIIEKDEPKIAEPSPFVSEKFPTPISDTDTKTEEKISPDISSETS
                     TGSKEVIMPESHEIMSSDSPGASAPTKADDSSEELWSPTGEKSEEPSAITPLSRDEEI
                     FE"
     gene            complement(1231..2043)
                     /locus_tag="P5123_10375"
     CDS             complement(1231..2043)
                     /locus_tag="P5123_10375"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRX16708.1"
                     /translation="MKQLFIILLIIATVPMNAGYRPYTEVSIQWDKGIVTARSSDSFE
                     ADVRLKKKGSGSINRLRLLSYSRAQKNTKELLASFLLGIPVEQGYTLEHLLDDSEYTR
                     EKISFMINNSVAFRQYPDGFYKAQCHASLTFKDIISTLPFRYHNENFPVYTEHGQSTL
                     YTSLIIDTRGTDFTPQLFPAILDEGGLEIYNRHKVNIASCGSSGIVNYVYDEESAISH
                     KIAGKHPYFTIALKTVDNKPVIGYKDVRRIFSNLKNSEYLKRCRVILIIDDL"
     gene            complement(2222..3211)
                     /locus_tag="P5123_10380"
     CDS             complement(2222..3211)
                     /locus_tag="P5123_10380"
                     /inference="COORDINATES: protein motif:HMM:NF015983.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HRX16709.1"
                     /translation="MKKNITIPFFIPHRGCPHQCVFCNQHSITGESNQISTELINQRV
                     MEYVADKDLVAQIGFFGGTFTALPLEEQETYLKAACNLKKSGHIHSIRLSTRPDSVSD
                     IICNMLKHYGVDTVEIGVQSFDNDVLYSSERGHSREDSINALKLLRKYGFRTGIQLMP
                     GLPSSTYQSDIESAKTAAKLNPDDLRIYPTVVIKNTRLAWLYQQGLYTPLSLEQAINI
                     CAQIYAIFEPLSTKIIRIGLHALSDAESVISGPYHTAFGFLVKSRFYRNIMEAKLPSS
                     SNENIKFSAELPAQNYEEYIGNKKDNILYLEKKYNIRINYHRGTNLRFEPVSL"
     gene            complement(3211..3750)
                     /locus_tag="P5123_10385"
     CDS             complement(3211..3750)
                     /locus_tag="P5123_10385"
                     /inference="COORDINATES: protein motif:HMM:NF016611.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HRX16710.1"
                     /translation="MKEKNILRLANYFDHHEIVDLTGNVDLILLHAYENMEIDSFQDA
                     ARLFYIYIDEKRESTEALNGLAVCFFEMEQYDHAQTIVDHALVLFPNDPVSLSNKASL
                     MWQEERYDEAVYYYQKSLSADPSMIESKVNLINLYREIGDLLLAYTLCLELCQTNPGD
                     SEINELLNSLLLDIAIFMH"
     gene            complement(3892..4146)
                     /locus_tag="P5123_10390"
     CDS             complement(3892..4146)
                     /locus_tag="P5123_10390"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRX16711.1"
                     /translation="MNISVKLLKNKDRFIATCPELEISCYASSQTEARQRIKDVISFY
                     LHSAKEMGLEVESFDSVMINGKYSGPISPLSIFETGSYLN"
     gene            4618..4956
                     /locus_tag="P5123_10395"
     CDS             4618..4956
                     /locus_tag="P5123_10395"
                     /inference="COORDINATES: protein motif:HMM:NF013231.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HRX16712.1"
                     /translation="MQDLCHVKEVMKEIYSFEKELREQSSLTINEALVLCSLLNSDSS
                     AGHLASEIGLSPARMSKVLSSLEKRELVVRSLGVRDKRIMEFKISNEGKKLITTLKEK
                     KVKVPDFSVI"
     gene            4988..>6183
                     /locus_tag="P5123_10400"
     CDS             4988..>6183
                     /locus_tag="P5123_10400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018336122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HRX16713.1"
                     /translation="MKKVLIVGGVAGGATTAARLRRLDENAQIILFERGEHVSFANCG
                     LPYYIGSTIPERESLFVMSPDGLEKRLNISVRIKNEVISIDRDKKEIRVKDISSGKEY
                     SENYDILLMSPGAEPLRPPIEGIDSNKIFTLRNVADSDKIKGYLDKNRPNDAVVVGAG
                     FIGLEMAENLHKLGIKVTIVEMLDQVMAPLDYEMACIAHNHLRVKGVDLQLGEQVKSF
                     AEKGDHITVNLESGKSIDTGIVILSIGVRPETKLAKDCGLELSDRGAILVNEYMQTSD
                     PNIYAVGDAIAFTNPLSGVITNTLLAGPANLQARIAAGNIAGYKKRTYRGAINTAIAK
                     VFDITAASTGLSEKYCKANNIAYVSNIIHAGDHAGYYPGAIPITIKTVFEPDGGKLLG
                     AQAVGY"
BASE COUNT         1868 a         1036 c         1286 g         1993 t
ORIGIN      
        1 aattgatctg atattttcga gagccttttc tcggttgtta gttttataga aacttcttgc
       61 aagataaagt ttagcctgca taataaaagg tgaagactga aacagcgact caagctcttc
      121 aaacttttct attgccatat tataatcacc gctgttatag gcatttattc ctgattcaag
      181 aatcacggtg tctccagaaa ggggagccgg gatgtccatt tgcgacctat tgcccgccaa
      241 gtctgattta gttgaagata tttgaccact tgtattgtca acaagttgtg attgatctga
      301 tgcagtttca ttttgctgga cgggtttgat acttggttgt aaggtctgtt cgtctgtcga
      361 actgttctgt ggctccgcga ttattctgat tattgcttcc gagcttttag gaaaagcagg
      421 tgtcgaaatt acaataatac aaatagttaa taaaatggga catgaccatt ttgattttaa
      481 taaatatgat atagtgcagt ttaaaaagtt tttcattcaa aaatctcctc gtcacgagat
      541 agcggtgtaa ttgcagacgg ctcttctgac ttttctccgg ttggagacca aagttcttct
      601 gatgaatcat ctgcctttgt cggggcagaa gctcctgggg aatcactgct catgatttcg
      661 tggctttctg gcatgatgac ctctttagag cctgtgcttg tttcagatga aatgtcaggt
      721 gatatttttt cttcagtttt ggtgtctgta tcactaattg gtgttgggaa tttttcagaa
      781 acaaacgggg agggttccgc aattttaggt tcatcttttt caataatatc tttgtgcgat
      841 gttaaaggag tgtcttcggt ttccggaaaa accctgactt cattactctc tgtatctttt
      901 tcaataagtt gttcagattc tatcatatca gcaaaacttg ctcgctcttt gtcacggctt
      961 atatctattt caaacacatc ccttgtagta ttaacgaggg gaactccacc tttatccgcg
     1021 gcttttttcc ccatgcttat tccggcaact acacccgcaa taacggatac aaatattatc
     1081 atgccgaaaa gctgacgtat ggtttgtttt tgacgtaccg gcattttttc gataaaacga
     1141 ttaaaagaag ccaatatcgt atacactatt ttcatggtta aacctttaca tggtaatatt
     1201 tcgaaatttc cattacagaa aagctaataa tcagagatca tcgataatta gtattactct
     1261 acatctttta agatactcac tattttttaa gttactgaat attcttctta cgtccttgta
     1321 tccaataact ggtttgttat ccactgtttt aagggcaatt gtaaaatatg gatgtttacc
     1381 tgctatttta tgagagatgg cgctctcctc atcatagacg tagttaacta ttccagatga
     1441 tccgcatgat gctatattaa ccttatgacg gttataaatc tcaagtccac cctcatcaag
     1501 tatcgcgggg aaaagctgtg gagtaaaatc agttccacga gtatcgataa ttagtgatgt
     1561 atacagagtc gattgtccgt gttcggtata aacaggaaaa ttttcgttgt ggtatctgaa
     1621 aggaagagtg ctaataatat ccttgaacgt tagtgatgcg tggcattgtg ccttatagaa
     1681 accatcagga tattggcgga aggcaacact attgttaatc ataaaagaaa ttttttccct
     1741 ggtgtattcg gaatcatcga gcagatgctc tagggtgtat ccttgttcga caggtatacc
     1801 aagaagaaaa gaggctagta gctctttggt gtttttctgt gcccttgaat acgagagcag
     1861 tctaagtctg tttattgatc cggatccttt tttctttaaa cggacatcgg cttcaaaaga
     1921 atccgaagag cgtgctgtaa ctattccctt gtcccattga attgatacct cagtgtatgg
     1981 gcggtaaccg gcattcatgg ggacggttgc aataattaga agtatgataa atagttgttt
     2041 cataatatat cttcccgaga aattcccttt cattatagta tatcgtatca tttgttaaaa
     2101 tatataaggt aaaaattatg tctcatggga aaaatttgca aaacataaag ttaggaatat
     2161 tgataataaa agaatcaacc attttaagat attatagagc gttattccag gtgtatcttg
     2221 attaaagaga tacaggttca aatcgtagat tagtgcctcg gtgataattg attcgtatat
     2281 tatacttctt ttctaggtaa agtatattat cctttttatt tccgatatac tcttcataat
     2341 tttgcgcggg cagttcggca gaaaacttta tgttttcgtt tgaagatgag ggaagttttg
     2401 cttccatgat gtttcggtaa aaacgcgatt ttactaaaaa tccgaatgca gtatgataag
     2461 gacccgaaat tactgattcg gcgtcagaga gtgcgtgaag acctattctt atgatcttag
     2521 tgcttaaggg ttcaaaaatt gcatatattt gtgcgcaaat gtttattgct tgttcaagtg
     2581 atagaggagt gtatagcccc tgctggtaga gccaggcgag tcgagtattt ttgattacaa
     2641 cggtgggata tattcgaaga tcatcaggat taagcttagc agccgtcttc gcactttcaa
     2701 tatctgattg atatgttgaa gaaggtaatc cgggcattag ctgtataccg gtcctgaaac
     2761 cgtatttccg taagagcttt aaggcattga tggaatcttc acggctatga ccgcgttctg
     2821 atgagtataa tacatcgttg tcaaaggatt gtactcctat ttcaactgta tctacaccat
     2881 aatgtttaag catattgcaa ataatatctg ataccgaatc tggacgggtt gaaagacgaa
     2941 tagagtgtat atgtcccgat ttcttgagat tacatgcggc ctttaaatag gtttcctgtt
     3001 cttccaaagg tagtgcggta aatgtgcctc cgaaaaaacc aatttgtgca acaaggtctt
     3061 tgtcggcaac atactccata actcgttgat taatcaattc ggttgatatc tggttggatt
     3121 cgcctgttat gctatgctga ttgcaaaaga cacactgatg cgggcatcct ctgtggggaa
     3181 taaagaatgg aattgttatg ttttttttca tcagtgcata aatatcgcta tatctagaag
     3241 cagtgaattt aataactcat ttatttctga atcacctgga tttgtctggc aaagttcaag
     3301 acatagtgtg taagctagaa gcagatcacc aatttcacga tacagattta ttaggtttac
     3361 cttggattct atcatggaag gatcagctga taatgatttt tgataatagt acactgcttc
     3421 gtcgtaacgt tcctcttgcc acattaaaga ggccttattg gaaagggata caggatcatt
     3481 agggaaaaga accagagcgt ggtctacaat tgtctgagca tggtcgtact gctccatctc
     3541 gaagaaacag accgcaagac catttaaagc ctctgtggat tctcgttttt catcgatata
     3601 gatataaaag aggcgtgctg cgtcctgaaa cgagtcaatt tccatattct cataggcgtg
     3661 cagaagtatt aggtcaacat tacctgtaag gtcgactatt tcatgatggt caaaataatt
     3721 tgcaagacgc agaatattct tttctttcat ttcacagata gcggatatat tagagtttta
     3781 aaaggcataa aatctgtgta taagctacga ctactgtttt attctgtcaa acagattgct
     3841 gataaactgg taaaaatatg aaagaagaga aaaaaataat tactattgat tttaatttaa
     3901 atagcttccg gtctcaaaaa tggaaagagg ggaaattggg cctgaatatt tcccattgat
     3961 catcacagag tcgaagctct cgacttcgag acccatctct ttagctgagt gcaggtaaaa
     4021 agagatgacg tctttgatac gctgtctagc ttcagtttga gagcttgcgt aacagctaat
     4081 ctccagttcc ggacatgttg caataaaacg gtctttgttc ttaagcagtt tcacagatat
     4141 gttcatattc aattcccgat tatatggttt taacctaagc ggcatatggt gatgcatttt
     4201 gcccgtcaaa caggtgatgc tattattacg taaagtgagt aataattaga tactattatg
     4261 attcagtaca gattattgac aacatctggg ctcttcaaac tagcaccttt attcccataa
     4321 tacttattaa taatcggatt atattctgct tatcttgaaa agaaagctga tgggaaaaaa
     4381 tttatttgtt atagatattt aataataaaa aatattgaca atagcaaata tttatgatat
     4441 taattaagta aggttgttgg gaacctctaa taaataactt tttttcaagg aacttgcgaa
     4501 aaattccttt aaaataggca ttaaaacagt gtgaccttca gaaatattgt aatttttcga
     4561 agttccctgt tattactttt aagttgtttt agtattaatt aaagcgaaag aggctatatg
     4621 caggatcttt gtcatgtaaa agaagttatg aaagaaattt acagttttga aaaagaactt
     4681 cgtgagcagt caagtttgac aataaacgag gctcttgtat tatgttcgct tctaaactct
     4741 gatagcagtg ctggtcattt ggcttccgaa ataggattgt ctccggcgcg aatgtctaag
     4801 gtcctttcgt cacttgagaa acgtgagctt gtagtcagat ctttaggagt tcgggacaaa
     4861 aggattatgg agttcaaaat tagtaatgag gggaaaaaac tgatcacgac cttaaaagaa
     4921 aaaaaagtga aggtacccga tttttcggta atttgaaact atgaaaattc taagtggaga
     4981 aaataatatg aaaaaggtgt tgattgtagg tggtgttgca ggaggagcta ctactgctgc
     5041 aaggcttaga aggcttgatg agaatgctca aattattcta tttgaaaggg gtgaacatgt
     5101 ttcattcgcc aactgtggtt tgccctatta tattggttcc acaataccgg aaagagaatc
     5161 tctattcgta atgagtccgg atggtcttga aaaaagatta aatataagtg tcagaattaa
     5221 aaatgaagta atatctattg accgtgataa aaaagagatt agggtaaaag acatatcaag
     5281 tggaaaagaa tattctgaaa attatgatat tttactgatg tctccaggtg ccgaaccact
     5341 tcgtcctcca atagaaggaa ttgattcgaa taaaattttt actcttcgaa atgtcgctga
     5401 tagcgataaa ataaagggat atttggataa aaacaggcca aacgatgccg ttgtggttgg
     5461 agcaggcttt attggactgg agatggccga aaaccttcat aagttgggca tcaaggttac
     5521 catagtagag atgcttgacc aagttatggc tccactggat tacgagatgg cctgtattgc
     5581 acataatcat ttacgtgtta aaggagtgga cctgcaactt ggcgagcagg ttaaatcttt
     5641 tgcagaaaag ggagaccata ttacggttaa tcttgaatcg ggcaaaagca tagatacagg
     5701 catagtgatt ctttcgatag gtgttcgtcc tgaaaccaaa ctggcaaaag attgcggttt
     5761 agaacttagc gatagaggag caatactggt caatgaatat atgcagacaa gtgatccgaa
     5821 tatatacgct gttggtgatg ccattgcatt tacaaatcca ttatcgggag ttattaccaa
     5881 cacactttta gcaggacctg ctaatcttca ggctagaata gctgctggta atattgctgg
     5941 gtataagaaa agaacctatc gtggtgcgat aaataccgca attgcgaagg tatttgatat
     6001 tactgcggct tcgacaggcc tgtccgaaaa atattgcaag gcgaataata ttgcttacgt
     6061 ttcaaatata attcatgccg gtgatcatgc gggttattat cctggagcta ttcccattac
     6121 tataaaaaca gtttttgaac ctgatggtgg taaattattg ggtgcacagg ctgtggggta
     6181 cca
//
LOCUS       DAOTGZ010000170         6144 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_3064
            NODE_15169_length_6144_cov_5.122680, whole genome shotgun sequence.
ACCESSION   DAOTGZ010000170 DAOTGZ010000000
VERSION     DAOTGZ010000170.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868088
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 6144)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6144)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:22:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,785
            CDSs (total)                      :: 2,751
            Genes (coding)                    :: 2,732
            CDSs (with protein)               :: 2,732
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 12 of 19
            Pseudo Genes (internal stop)      :: 5 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6144
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15169_length_6144_cov_5.122680"
                     /isolate="AMR_MDS_3064"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            45..494
                     /locus_tag="P5123_10405"
     CDS             45..494
                     /locus_tag="P5123_10405"
                     /inference="COORDINATES: protein motif:HMM:NF012579.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HRX16714.1"
                     /translation="MSDIIEYTSTDFIKIVESEDKFGVIEELASLFSEGDVCTDIKAL
                     IAALKDRERIMSTGIGFGIAIPHAKIEQVKNISFAVGISKKGVEFDSMDGKPVHLIIL
                     VAAGDKQHKHYLSLMSNIMTVLKNDEIKEKIIDSTDKVEILRLLGSC"
     gene            505..885
                     /locus_tag="P5123_10410"
     CDS             505..885
                     /locus_tag="P5123_10410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011815048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2721 domain-containing protein"
                     /protein_id="HRX16715.1"
                     /translation="MNLTTPALLFPALSLLLLAYTNRFLTLAGLIRNLCKERSVSNLP
                     EIDGQIKNLRKRIYLIKYMQFLGIASLFFCVISMFFLFLNKELMGEIVFGISLVLMMA
                     SLGFSMREIHISVDALDIQLKSCK"
     gene            complement(957..1439)
                     /locus_tag="P5123_10415"
     CDS             complement(957..1439)
                     /locus_tag="P5123_10415"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRX16716.1"
                     /translation="MEITDFRTSIDVEVSKLEKIENYAQKYNMDISDVMNTVLDMYSK
                     KLAKNPVCNDTLNRYQERGTNYKSVHIRFSSITKRKLKDIANLYCYSLSYMLFLAMDF
                     FEEFMNGVKEFVRPVFHAAFVHHFFGFPYFINSWGMNFYEGQDQPAGQESQKRRPLLC
                     "
     gene            1918..5376
                     /locus_tag="P5123_10420"
     CDS             1918..5376
                     /locus_tag="P5123_10420"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRX16717.1"
                     /translation="MFKEYKDLDDKFKKRIVSQGHGFVCLDGYEDQFYKIESYDKMDP
                     FFMTLTSPEDHWIFISSTGGLTAGRKNSDNAIFPYYTDDKVSENHKNTGSKTIIRVKD
                     EKPVIWEPFSDRYFGVYDIKRNLYKNRLGNILVFEEINFDLGLVFTNAWSSSPKYGIV
                     KISNIANISNREISFSIIDGIRNTLPAGVTEQVQSQMSNLLNAYKRAELAPVKKCLLG
                     IFSLTATLTDLAEPSESLKSTTWWHAGFEPDAVLLSSSQLDRFRKTGMVDTENDIKGS
                     RGAFFVKKDYELPANTRADWIMAGEVNQTHTSICSLMKFLDNKNNNHYGEVMDDINLA
                     LKSLEKIIGVNDGLQMTGDMMTSVHHSANVLFNVMRGGYFNNGYMIQMEDLSSFIKEH
                     SKETYRKFESFLTSFAGEVHFSDVVESADQSGDSDLSRLVREYLPVTFSRRHGDPSRP
                     WNKFSINTRNEDGSARIDYQGNWRDIFQNWEALLFSNPRFAENIFCKFLNAVTADGYN
                     PYRISRSGIDWEKPEPENPWANIGYWSDHQIIYLLKLLELEQKFNPDSLKNILNRPIF
                     THANVPYEIKKFEDVVNNPYDTIVFNEQKDDLIAERVLKFGSDGRLLFASEGELVHTS
                     MVDKLLILLLAKMSNLVPDGGIWMTTQRPEWNDANNALVGKGVSVVTLSYLYRFVGFF
                     SQIISNNDEDLYNINSESYNWYKSEDKVLNTYRTALEKGFDDKNRFAFMRESQKSADE
                     YREILYKKGLTNSVISISRVELISFIENVNAYLKKSLENSIREDGLFHAYNTLEIRGE
                     SAVISRLYEMLEGQVAGISSGLLNAQQSDTIFNDLKKSSMYREDQHSYMLYPNRDLKG
                     YLDKNRFDYEKISNTSLKEKLLSGKLKTIIEKDANTICHFNSSFRNVGDLNRAIENLN
                     QFENDDVVNSNEKQILNQLFEEVFNHASFTGRSGTFYAYEGLGSIYWHMVSKLLLAAQ
                     ETVFVATNNDLAKKLANRYFDIRNGIGFNKSPEVYGAFPVDPYSHTPWGAGAKQPGMT
                     GQVKEEVLTRFAELGLRILEGEMVFDDTLVKEEEFLKNDSDFTYYNEKGQECCLKLSA
                     GSYGFTFCQLPIVVHKKNDSHRSSIKLFMHDGTEKNIEGNRINKPLSASVFSKEAICE
                     RIEVIL"
     gene            5610..>6144
                     /locus_tag="P5123_10425"
     CDS             5610..>6144
                     /locus_tag="P5123_10425"
                     /inference="COORDINATES: protein motif:HMM:NF021454.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2167 domain-containing protein"
                     /protein_id="HRX16718.1"
                     /translation="MKFLSRGLFAAVCYIVISIFSLNLTVSAFADSEENEEQITIDWV
                     AGPADADIGNLAKLKLPEGYVFANGDDTRKIMEYYGNQITDRELGYIAPQDQSWFMVF
                     EFEETGYIKDDEKENLDQDAILDSIKEGTKQGNEWRKQNNIPEMEVVGWHTAPFYNAK
                     TNNLEWGIEFSSNGNQVI"
BASE COUNT         1935 a          985 c         1273 g         1951 t
ORIGIN      
        1 aagcaaaatc taaacgtaaa cgaaaatagg aatggaatat tatcatgtct gatattatag
       61 agtatacatc taccgatttc attaaaatag tagagtctga ggacaagttt ggagttatcg
      121 aagagcttgc ctcgctcttt agtgagggtg atgtatgtac cgatataaaa gcgctgattg
      181 cagcgcttaa agaccgtgaa agaataatga gtacaggtat cggttttggg attgctatac
      241 ctcatgctaa aatcgaacag gtaaaaaaca tctcttttgc ggttggaata tcaaaaaaag
      301 gtgttgaatt tgactcgatg gacggaaaac ccgttcatct gattatactt gtagctgctg
      361 gcgataagca gcacaagcac tatctttcgc tgatgtccaa tataatgacc gtacttaaaa
      421 acgacgagat aaaggaaaaa attatcgata gtaccgacaa ggttgaaata ttgcgacttc
      481 tgggttcctg ttaagaggtt aaaaatgaat ttaacaacgc ctgctctgct ttttcccgct
      541 ctttcacttt tgcttttggc atataccaac cgttttttaa cccttgctgg cttgatccgt
      601 aatctttgta aagagagaag tgtttcaaac cttcccgaaa tagacggcca gataaaaaat
      661 ttgaggaaaa ggatctatct tatcaagtat atgcagtttt tgggtatcgc tagccttttc
      721 ttttgtgtaa taagcatgtt tttccttttt ttaaacaagg agcttatggg cgaaattgtt
      781 ttcggaataa gccttgtgct aatgatggca tcacttggat tttcgatgag agaaatccat
      841 atttctgtcg atgctctcga tatccagctt aaaagttgca agtgattttt gttttcagtg
      901 tttgctttta tttgtggttt ctgtttttat gagtagaaat cagttataaa agcaatctaa
      961 cacagtaagg ggcgtctctt ttgggattct tgtccagcag gttggtcttg cccctcgtaa
     1021 aagttcatac cccagctgtt tatgaagtac ggaaaaccaa agaagtggtg aacaaaagca
     1081 gcatgaaaaa ctggtctgac aaactccttg accccattca taaattcctc gaagaaatcc
     1141 attgctagaa acaacatata cgacaaacta taacagtaaa gattggctat gtccttcagt
     1201 tttcttttcg taatactcga aaatcgaata tgcactgatt tatagtttgt acctctttct
     1261 tggtacctgt taagtgtatc gttgcatact ggattcttag ccagtttttt tgaatacata
     1321 tccagaactg tattcataac atccgatata tccatattat acttctgtgc gtaattttcg
     1381 attttttcca acttactgac ttcgacatct attgatgtcc taaaatctgt aatttccatt
     1441 tataacctcc agattatttc tataaatgta tactggtcta aagggagttt tgttaaaata
     1501 attttatatt tttttaccgt tcattgcctg ttttctctta cagtgtgggt atttattttt
     1561 acaaacggtc ttttctgttc ttcattttat aaagtttaaa caattgagca gtcttttagt
     1621 gaattgtaga cctttttgga tgattagcac gaatatccat aaacaaatca ctaatactgt
     1681 agagttttgg cgacatgaag tttaaaaggt cttttagata tttgtataga gtacatcggt
     1741 tattgacgca tgctgttcaa aatgtggtga ttttttctta gtttcttata ttaataagta
     1801 attgaataac gattttttga atttctttga taaaaaatct atgttacact tgtaattcat
     1861 tgacattgtt ggtataatct aattagttga cacaagaaac aaatagtggg ggttgtaatg
     1921 tttaaagaat ataaagattt agatgataag tttaaaaagc ggattgttag ccaggggcat
     1981 ggctttgtct gtctggacgg gtatgaagat cagttttata aaattgaatc ttacgacaaa
     2041 atggatcctt tttttatgac attaaccagc cctgaggatc attggatttt tatttcatct
     2101 acaggaggtc ttacagctgg tcgtaaaaat tctgataacg ccatctttcc atattatacc
     2161 gatgataagg ttagtgaaaa tcataaaaat acgggtagca aaacaataat tcgtgttaaa
     2221 gatgaaaaac cggtaatttg ggagcctttt agtgatcgct actttggtgt ttatgatatt
     2281 aaacgtaatc tttacaagaa ccgtttgggc aatatccttg ttttcgaaga gataaatttc
     2341 gacttgggac ttgtttttac aaatgcatgg agcagcagtc caaaatatgg tatagtaaag
     2401 atctcgaaca tagcaaatat atccaataga gaaatatctt tctcaattat tgacggtata
     2461 cgtaataccc tgccagcggg tgtaaccgaa caggttcagt cacagatgag taatttgctt
     2521 aatgcgtaca agagagcaga gcttgctcca gtgaaaaaat gcttgcttgg aatattttct
     2581 ctaacagcaa cgctgacaga ccttgcggaa ccaagcgaat ccctaaaatc aacaacgtgg
     2641 tggcatgccg gttttgaacc cgatgctgtg cttctctctt cttcgcagct agatagattc
     2701 cgcaaaaccg gcatggtaga taccgaaaat gatataaaag gaagccgagg ggcgtttttt
     2761 gtaaaaaaag attacgagtt gccggcaaat accagagccg attggattat ggcaggagag
     2821 gtaaaccaga ctcatacatc aatatgttcc cttatgaagt ttcttgataa caagaataat
     2881 aaccattacg gcgaagttat ggacgacata aacttggcct taaaatcgtt ggaaaaaata
     2941 atcggagtca atgacggtct tcagatgact ggtgatatga tgacttcggt gcatcatagc
     3001 gctaatgttc tttttaacgt tatgcgtgga ggatatttta ataatggata tatgatacaa
     3061 atggaagact tatcttcttt tattaaagaa catagcaaag agacttatcg aaagtttgaa
     3121 agctttttga catcttttgc aggcgaggtg catttttccg atgttgtcga atctgcagat
     3181 caatcaggag actcggatct ttcaagactg gttcgcgaat acctacctgt tacgtttagt
     3241 cgacgtcatg gtgatccaag ccgtccatgg aacaagtttt caataaatac ccgcaatgaa
     3301 gacggtagcg cacgtatcga ttatcagggg aactggcgtg atatctttca aaactgggag
     3361 gcgcttcttt tcagcaatcc gcgtttcgcg gaaaatattt tttgcaagtt tttaaatgct
     3421 gttacggctg atggatacaa tccttatcgt atatctcgct caggaataga ctgggaaaaa
     3481 cccgagcctg aaaatccttg ggcaaatata gggtattgga gtgatcatca gataatttat
     3541 cttttaaaac tgttggagct tgagcagaag tttaacccgg attcgctaaa aaatatattg
     3601 aataggccca tatttactca tgccaatgtt ccgtatgaaa ttaagaagtt tgaagatgtg
     3661 gtaaataatc catacgatac aatagttttt aatgagcaaa aggatgattt gattgccgag
     3721 cgtgtattaa aatttggatc tgatggccgt cttctcttcg cgtcggaagg tgaactggta
     3781 catactagta tggttgataa gttgttgatt cttcttctgg ccaagatgag caatttggtc
     3841 cctgatggcg gtatctggat gaccacccaa aggccggagt ggaacgacgc aaataatgct
     3901 cttgttggca agggggtttc tgttgtgact ctatcctatt tgtatcggtt tgttggcttt
     3961 ttttcacaaa ttataagcaa caatgatgaa gatttgtata atataaatag tgaaagctat
     4021 aactggtaca agtctgaaga taaggtttta aacacatata gaacagctct tgaaaaagga
     4081 tttgatgata aaaaccgttt tgcgtttatg cgtgaatccc aaaaatcagc tgatgagtat
     4141 cgtgaaatac tatataaaaa gggtttaaca aattctgtca taagtatatc tcgagttgag
     4201 ctaatttctt ttattgaaaa tgtaaatgcc tatttgaaaa aaagtttaga aaatagtatt
     4261 cgtgaagatg ggctttttca tgcttacaat accttggaga taagaggaga aagtgctgtt
     4321 atatcacgac tttacgaaat gctcgaaggc caggttgccg gtatctcatc cgggctccta
     4381 aatgcacaac aatctgatac aattttcaat gatcttaaaa aaagttcgat gtacagagaa
     4441 gatcaacatt catatatgct ttatccaaac agagacctaa agggctatct tgacaaaaac
     4501 agatttgatt atgagaaaat ttcaaacacc tctcttaaag aaaagttact tagtggtaaa
     4561 ctcaaaacaa taatcgaaaa ggatgccaat actatttgtc attttaacag cagtttcaga
     4621 aatgttggcg atcttaatcg ggcaattgag aatttgaacc agtttgagaa tgatgatgtt
     4681 gtaaactcaa atgagaagca gatattaaac cagcttttcg aagaggtatt taatcacgct
     4741 tcttttacgg gcagatcggg tacattttat gcttacgaag ggcttggcag catctattgg
     4801 catatggttt ccaagttgtt gcttgccgcg caggagactg tttttgtggc aacgaataat
     4861 gatttggcta aaaaactagc caatagatat tttgatattc gaaatggcat cgggtttaac
     4921 aagtctccag aagtgtacgg agcatttcca gttgatccat attcgcacac tccctgggga
     4981 gcaggtgcca aacagccggg gatgacgggg caggtaaaag aagaggttct aactcgcttt
     5041 gctgaattag gacttcgaat cttggaaggt gagatggttt ttgatgatac cttagtcaag
     5101 gaagaggagt tccttaaaaa cgattcagat tttacatatt ataatgaaaa aggccaagag
     5161 tgttgcttaa aactaagcgc ggggagttat ggattcacgt tttgccaact tccgattgtt
     5221 gtgcataaaa aaaatgattc gcaccgatct tctataaagt tgtttatgca tgacggaaca
     5281 gagaaaaata ttgaaggcaa cagaattaac aaaccgcttt ctgcatctgt tttttccaaa
     5341 gaagctattt gtgagagaat agaagttatc ctttaaacag cttcagcaat tttattttct
     5401 cccacataaa tatttgtggg agaaaagatc ttctttccag aaatatgtac taggtaaaaa
     5461 aatattgatt ttaaaaactt tattgactat caatttaaat atataatatg gtggctaatt
     5521 aaaaattgca actgatttgt ttagagaaag tctgttttct gtggttgttt atttatatcg
     5581 tattaaaatg tgatagagag gttgatattg tgaaattttt atcaagaggg ttatttgccg
     5641 ctgtttgtta tattgttata tctatttttt ccttaaactt aacagtgtcg gcttttgcgg
     5701 atagcgaaga aaacgaggag caaatcacta ttgattgggt tgctggccca gctgatgccg
     5761 atattggaaa tttggcaaag ttaaagttgc ctgaggggta tgttttcgcg aatggcgatg
     5821 atacacggaa aataatggag tattatggaa atcagataac cgacagggag ttggggtata
     5881 ttgcacctca ggatcagtct tggtttatgg ttttcgaatt tgaagagacc ggttacataa
     5941 aagacgacga aaaagaaaat ttggatcagg atgcaatact tgattcaata aaagagggta
     6001 caaaacaggg aaacgaatgg cgcaaacaga ataatatacc ggaaatggaa gtcgttggct
     6061 ggcacactgc ccctttttac aatgcaaaaa caaataatct tgagtggggc attgaatttt
     6121 cgagcaacgg gaatcaggta atta
//
LOCUS       DAOTGZ010000171         6090 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_3064
            NODE_15353_length_6090_cov_4.637614, whole genome shotgun sequence.
ACCESSION   DAOTGZ010000171 DAOTGZ010000000
VERSION     DAOTGZ010000171.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868088
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 6090)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6090)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:22:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,785
            CDSs (total)                      :: 2,751
            Genes (coding)                    :: 2,732
            CDSs (with protein)               :: 2,732
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 12 of 19
            Pseudo Genes (internal stop)      :: 5 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6090
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15353_length_6090_cov_4.637614"
                     /isolate="AMR_MDS_3064"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            58..666
                     /locus_tag="P5123_10430"
     CDS             58..666
                     /locus_tag="P5123_10430"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="GAD-like domain-containing protein"
                     /protein_id="HRX16719.1"
                     /translation="MNQDSLFCTMFGKAIDSEPLSSENLKKINEKLNLPIFLKELLHS
                     DGFGGYADDFLWTCDPTYLMNFTQNAISSDYSAIPFMRTSFGDIIFYLRKDNTDIFHF
                     CALNFRHRELVHLFNGSCEDFFNTILTEYGFLFHKLKINLDLYWFTFEKLGAADRNSC
                     YGYITEPADGGKDCPENITKYIVSEYFEKASAMIKTPLGIHI"
     gene            884..1354
                     /locus_tag="P5123_10435"
     CDS             884..1354
                     /locus_tag="P5123_10435"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRX16720.1"
                     /translation="MKKAVICLFILILATAGCSKASDAIDDAIDSTSSAVPGYAEMAN
                     VLNDYTDIQEKYLKDLKNVDSPEDLIKALETYTENMMKIVPMMQELSEKYPELSEVDP
                     PKHIQERLDKIANEIQEEAPKAVMEYQSDPKVQEAFMKMASVMGQSMEEAEGEE"
     gene            complement(1452..2129)
                     /locus_tag="P5123_10440"
     CDS             complement(1452..2129)
                     /locus_tag="P5123_10440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007723138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0015969 - guanosine tetraphosphate
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP pyrophosphokinase family protein"
                     /protein_id="HRX16721.1"
                     /translation="MASDLLIQNQTQFNFEEFFMPYDLAVQTFIIKLEGIRQQYRKKG
                     LHSPIESVTGRVKSCQSIIDKIGLENINEQNLEALYDIAGIRILCKYVDDIAVVAEML
                     RNRRDVDVIKEKDYVHNSKSSGYRSYHLMSNYLVETIDGVKPVRIEFQIRTHAMHLWA
                     TVEHRLKYKYKGEIPPEIRERLVRAASATVSLDEELSAIKNEIEEARSEFTANRGSIC
                     DLMHWNY"
     gene            complement(2404..3084)
                     /locus_tag="P5123_10445"
     CDS             complement(2404..3084)
                     /locus_tag="P5123_10445"
                     /inference="COORDINATES: protein motif:HMM:NF012321.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HRX16722.1"
                     /translation="MERLLKFFMVVTFVSVLSVITACDLEGLKAIVGNYEDVYDEFKQ
                     IADQTGGKIIEAEDAEDVPEQIVQVIKQYAQGQIEAEIMLLIDKTGSMEDDIDAVVEG
                     LNLIIDELPDNARLGLATYGDKNVDGSEWFSFVDLTSDHDTIKGELDKIETTGGGDWR
                     ESVFDGIYETIDRATWWDSAPLRLVICIGDAAPLTGSDTTYSLDDVVTKSKELDPAVN
                     VFMIAIKN"
     gene            complement(3313..4299)
                     /locus_tag="P5123_10450"
     CDS             complement(3313..4299)
                     /locus_tag="P5123_10450"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007810883.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HRX16723.1"
                     /translation="MNYRKLGKTGFDVSEVSLGTWQVGGRWGSGFDDANAEKIINEAI
                     DRGINFIDTADVYEDGKSEAAVGRVVRSRSERVYVATKCGRFINPHVAEGYTPEALRK
                     FVENSLINSGLETLDLIQLHCLPTEVYYRPEIFELFDKLKDEGKILNLGVSVEKVEEA
                     LKAIEYDNVTTVQIIFNLFRQRPSELFFSEAKKKQIGIIARVPLASGLLTGKFSLDTV
                     FDKSDHRYSNRNGEWFDKGETFAGIDYSKGVKAVDRLREVFPDEENLAPRALQWILSF
                     SEVSTIIPGASRVEHLISNLKTLSHNELSQNEIDRMNAIYSEEIQNLVHHLW"
     gene            4672..5892
                     /locus_tag="P5123_10455"
     CDS             4672..5892
                     /locus_tag="P5123_10455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013252644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FprA family A-type flavoprotein"
                     /protein_id="HRX16724.1"
                     /translation="MKAVEIAENIYFIGANIKNGDLFEGMWPIPDGVSLNSYIVKGEK
                     TALIDLVRDWDNAPGALKEQLASLNIDINNIDYLVLNHLEPDHTGWLCEFLKLNPKVT
                     ILTTQLGKNLVKGFYKNEDNVQIVKDGEKLDLGNNMELTFYTAPNVHWPETMVTYNEK
                     SGVLFSCDAFGSYGCVDNPFDDQLDDEKHAFYDRESLRYYANIVASFSPFVQKAIDKL
                     ASLDIKMIAPSHGIIWRENPSVIIERYRRFAEYMNGPAEPEITIIWGSMYGNTESALA
                     PLIDGIKSEGVTVHLHQVPNEDVSFPLTSAYKSAGLVFAMPTYEYRMFPPVAAAIDMF
                     GRKHLKNKKVLRIGSYGWSGGAQKEFDSITADYNWDCLEPVEWQGGCDEDTKKIIYEQ
                     GKKLAKTVKEFCGA"
BASE COUNT         1978 a         1193 c         1043 g         1876 t
ORIGIN      
        1 gaaacggcag cgactgattt tcatgtaatt taatatctat caatcgggga gcttagagtg
       61 aatcaagatt ctttgttttg tacaatgttt ggtaaggcaa tagattcgga accgttaagt
      121 agtgaaaatc ttaaaaagat aaacgaaaaa ctgaatctgc caatttttct aaaagagtta
      181 ttacattcag atggatttgg cgggtatgca gatgattttt tatggacgtg tgatccaaca
      241 tatcttatga atttcactca aaatgcaatt tcctcggatt attctgctat accttttatg
      301 agaacctcct ttggagatat tattttttat ttaagaaagg acaataccga tatttttcat
      361 ttttgcgcgc taaattttag acaccgtgaa ctggttcatc tttttaacgg ttcttgcgaa
      421 gattttttta acacgatatt aaccgaatac ggattcttgt ttcataaact taaaattaac
      481 ttggatcttt actggtttac attcgaaaaa cttggcgcag ctgacaggaa cagctgttat
      541 ggttatataa ctgaaccagc agatggagga aaagactgcc cagaaaatat tacaaaatac
      601 attgtttcag aatattttga aaaggcatct gctatgataa aaactccact cggcatacat
      661 atataaccga aatcggcaca gttttctcaa ttaatatttc ttgaaaaatt ttatacttca
      721 tatttttatt ttacttgcaa tctgaactcg aaaagatata aatgtggcgg gtttttgata
      781 ctttttactc tttttttgtt ataaatataa agagtcttat tgaagtatta tttaagagcc
      841 aaataatgta ttcctctgct tcacacaatt taaggagaaa ataatgaaaa aagccgtaat
      901 ctgtcttttt atactgattc ttgccactgc aggttgctca aaagcttcag atgctattga
      961 cgatgctatt gattctacct cttcagccgt acctggatat gccgagatgg caaacgtgtt
     1021 aaacgattat accgacattc aggaaaaata tcttaaagat ctaaaaaatg tcgacagccc
     1081 tgaagacctg atcaaggctt tagagaccta tactgaaaac atgatgaaga ttgtaccaat
     1141 gatgcaagag ttgtctgaaa aatatcctga gctatcagaa gttgatcctc caaaacacat
     1201 tcaagaaaga cttgataaga ttgcaaacga aattcaggaa gaggcgccga aagctgtgat
     1261 ggaatatcag tctgacccta aagttcagga agcctttatg aagatggcaa gcgtgatggg
     1321 ccagagcatg gaagaagctg agggcgaaga gtaaaaaaaa ttacatttat caagaatttt
     1381 taaaagagaa agagcgccta tggtgctctt ttttatacta agcttttaat aacgtttgat
     1441 tcataaaaaa atcagtagtt ccaatgcatc agatcacaaa tactgccacg gttggcggta
     1501 aactcggatc gtgcttcctc gatctcattc ttgattgccg aaagctcctc atctagagac
     1561 accgtggccg aggcagcacg cacaagcctc tcccttattt caggaggaat ttcgccctta
     1621 tatttgtatt ttagccggtg ctccactgtt gcccagagat gcatagcgtg ggttcttatc
     1681 tgaaactcta tacgaacagg tttaactccg tcaatggtct ctacaagata atttgacatt
     1741 aaatgatacg atctgtaacc acttgatttt gagttatgaa cataatcttt ttcctttatt
     1801 acatcaacat ccctgcggtt gcgaagcatt tcagcaacaa cagcaatatc gtcaacatac
     1861 ttgcatagta tacgaattcc ggctatatcg taaagcgctt ctagattttg ttcattaatg
     1921 ttttccagcc ctatcttgtc tattatactt tgacagcttt tcaccctgcc agtaacagac
     1981 tcaattggcg aatggagccc ttttttgcga tactgttgcc tgattccttc aagcttgatt
     2041 ataaaggtct gaacggcaag atcgtatggc ataaagaact cttcgaaatt aaactgtgtc
     2101 tgattttgaa taagcaaatc actcgccata attcaccgcc caatatccaa taaaataatt
     2161 atactataaa tgtgcactca acaaacatta aatcatgaga ttatcagttt ttcaagggtt
     2221 tttaacaaat aagagaaaaa tttacaaaat agatgcttaa tttactaatc agtggttatt
     2281 gaaaatgcaa attaagtaaa taaaggcaca ttcttgatag atgtataaaa aaaggggggt
     2341 tgccctaata tgcgcccccc attttttata ttactaaact gaaaaaagct ttgttgaaaa
     2401 ttctcagttc tttatcgcga tcatgaaaac atttacagcc ggatcaagtt ctttggattt
     2461 tgttaccaca tcatcaagag aataggtcgt atcgctacct gtaaggggag ctgcgtctcc
     2521 aatacatatt accaaacgta atggtgcact gtcccaccat gtcgcacggt cgattgtttc
     2581 ataaataccg tcaaatactg attcacgcca atcgccacca cctgtagtct caattttgtc
     2641 aagttctccc tttattgtgt catgatccga tgttaaatcc acgaaagaga accattcact
     2701 accatcgaca tttttgtcac cgtatgttgc aagcccaaga cgagcattat caggtaattc
     2761 atcaataata agattcaatc cttcaacaac agcatcgata tcatcttcca tactacctgt
     2821 tttatctata agcaacataa tctctgcctc gatctgtccc tgtgcatatt gttttataac
     2881 ctgaacaatc tgctccggca catcctcagc atcttctgct tcaatgattt ttccaccagt
     2941 ctgatccgca atttgcttaa actcatcata aacatcttcg tagttaccta caatagcttt
     3001 aagaccttca aggtcacatg ctgtaataac acttagaaca gaaacaaacg taaccaccat
     3061 aaaaaatttc aataatcttt ccatttcttc accccaattt attactactt ttataaaaac
     3121 agtagcaaaa taccaaattt aacctataaa acagataacc tataccaaaa cgctactttt
     3181 ttttaaacta atgattacga aatgtctata aattttaatt tatattgatc taccaatcat
     3241 ttaatgttaa aaactgacta gcgttttttt ccgaacaaca agcatggcaa cctacaaaat
     3301 attcaatgtt tttcaccaaa gatgatgaac aagattctga atctcttcgc tatagatagc
     3361 attcatacgg tctatctcgt tttgggaaag ttcattatgc gaaagagtct ttaaattgct
     3421 tatcagatgt tccacacgtg atgccccggg tataatagtg gacacctcgg aaaaagacaa
     3481 gatccattga agtgcacgag gagcaagatt ctcttcatca ggaaaaactt cgcgcaatcg
     3541 atccacagcc tttacaccct tgctatagtc tattcccgcg aaggtttcac ctttgtcaaa
     3601 ccactccccg ttacgattag aataacgatg atcagatttg tcaaaaactg tatccagaga
     3661 aaattttcct gttaacagac cactggcaag aggaacacgc gcaataattc cgatctgttt
     3721 ttttttggcc tctgaaaaga acaactctga tggacgttgc ctaaaaagat taaaaataat
     3781 ttggacagtt gttacgttat cgtattcgat cgccttaaga gcctcctcca ccttttcgac
     3841 actaacccca agattcaaga ttttaccttc atctttaagc ttgtcaaaaa gttcaaaaat
     3901 ttcaggtcga tagtatactt cagttgggag acagtgaagc tgaataagat caagagtctc
     3961 caaaccgcta tttattaggc tgttttcgac aaattttctt aatgcctctg gcgtatatcc
     4021 ctccgccaca tgaggattta taaaacgacc acattttgta gcgacataaa cgcgttcact
     4081 tcttgaacga actaccctgc caaccgctgc ttcactcttg ccatcttcat atacatcagc
     4141 ggtatcaata aagtttattc cccgatcaat tgcttcgttt attatctttt cagcatttgc
     4201 atcatcaaac ccgctgcccc agcgccctcc tacctgccat gtgccaagcg atacctctga
     4261 tacatcaaaa ccagttttgc ctaattttct atagttcatc tttaccccca gaatttcctt
     4321 tattttttta aaacgacaaa gaagcttttc tttattgccg tttttaaaag ttcttaaaat
     4381 attgtccaaa tagaagtaaa aaagttcatt ttataatttc ataacatata tattggcaaa
     4441 tttctagatt ttttcataga acttttttaa tacagaataa tataatggta attattaggt
     4501 aaacaagact tcagtttgac taataatctt atttcttaac ctttaaaata ttttgtttca
     4561 ttacaagctc attgttctga caagaaaggc ctaaaaatga aaatttcttg ttgcaaagaa
     4621 taatgttaac gattatcatt atcaaataca atatataaaa gaggaaccta aatgaaagca
     4681 gtggaaatag cagaaaacat ctattttatc ggagcaaaca taaaaaacgg ggatctattt
     4741 gagggcatgt ggccgatacc ggacggagta tccctaaact catatattgt taagggagaa
     4801 aaaacagcgc ttattgacct tgttcgtgac tgggataatg ccccgggggc tctaaaagaa
     4861 caactggcct ccttgaatat cgatatcaac aatattgact atcttgtact gaaccatctt
     4921 gaaccagatc acactggatg gttatgtgaa tttttaaagc ttaatcccaa ggtaacaata
     4981 cttacaactc agcttggaaa aaacttggta aagggatttt ataaaaatga ggacaacgtc
     5041 cagattgtaa aagatggaga aaaactggat ctaggaaata atatggaact gaccttttac
     5101 acggctccaa acgtacactg gcccgaaacc atggtcactt ataatgagaa gtcgggagta
     5161 ctgttctcat gtgacgcttt cggatcatac ggctgtgtag acaacccttt tgatgatcaa
     5221 ctggatgatg agaaacacgc tttttacgat agggaatcac ttcgctacta tgcaaatatt
     5281 gtagcgagtt tctctccatt tgtacaaaaa gcaatagata aactcgcctc tttggacata
     5341 aagatgattg ctccttcaca tggcattatc tggcgagaaa atccctcggt aataattgag
     5401 cgatatagac gattcgccga atacatgaac ggcccagccg aacctgaaat caccataata
     5461 tggggctcaa tgtacggtaa tacggaatcc gctttggcgc cactaattga tggtataaaa
     5521 tcagaaggag taactgtaca cctgcatcag gtaccaaatg aagacgtatc ctttccactg
     5581 acttcggcct ataaatcagc cggtcttgtt ttcgcgatgc ctacgtacga ataccgtatg
     5641 tttccacctg ttgctgcggc tattgatatg tttgggcgaa aacatctaaa gaacaaaaaa
     5701 gtgctgcgta tcgggtcgta tggctggtca ggtggtgctc aaaaagaatt tgattcaatt
     5761 acagctgact ataactggga ctgtcttgaa cctgtcgagt ggcaaggcgg atgcgatgag
     5821 gatactaaaa agataattta tgaacagggt aaaaagctgg caaaaacagt aaaagagttt
     5881 tgtggcgcat aatttgatga aactaaaata gtattaacat cgatataata tttgttacta
     5941 attttaagta tgtcgggaac aaacgcgata aaatataatt tatgtaacga attattactc
     6001 tttatgtgtt ttaattttat aatcactttt tataacataa tttacagatc ccgatacgga
     6061 gtgttcaaga catgttataa aaagtgatta
//
LOCUS       DAOTGZ010000172         6040 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_3064
            NODE_15510_length_6040_cov_4.120802, whole genome shotgun sequence.
ACCESSION   DAOTGZ010000172 DAOTGZ010000000
VERSION     DAOTGZ010000172.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868088
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 6040)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6040)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:22:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,785
            CDSs (total)                      :: 2,751
            Genes (coding)                    :: 2,732
            CDSs (with protein)               :: 2,732
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 12 of 19
            Pseudo Genes (internal stop)      :: 5 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6040
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15510_length_6040_cov_4.120802"
                     /isolate="AMR_MDS_3064"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            454..1005
                     /locus_tag="P5123_10460"
     CDS             454..1005
                     /locus_tag="P5123_10460"
                     /EC_number="2.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024812.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family protein"
                     /protein_id="HRX16725.1"
                     /translation="MVTIQTERLIIRDNLKKDLNDLYALTSDPVAMRFMKSTKIESIS
                     EAKKNLKQSINESKKKNREKYYFAMINRSTNEYIGAIGYIVEAVYPQGKLVELGYFIK
                     QQYWRMGYTTEAGSAVIKFAFENDNVIKIVAACLKENCASENTMIKLGFRKEGELNNY
                     QFHENIWKTRVFYGLLKEDYLAG"
     gene            1488..2510
                     /locus_tag="P5123_10465"
     CDS             1488..2510
                     /locus_tag="P5123_10465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015722107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SPFH domain-containing protein"
                     /protein_id="HRX16726.1"
                     /translation="MFGFKFIKFQPTDYVLRYKNGKIVKEGLGLSFFYYAPRTSLVSV
                     PIGSAEVPFIFEEVTTDYQAITIQGTVTYKIAEPKKIAEYLNFTLDYTGSSYLSDDPQ
                     KLPQRIINAVHVLTKKEMQNLSLREAIRSSEALVASVSQGLGKSTEITSLGIKVLGIS
                     ILAIKPNKETARALEAEAREQILKEADDAIYTRRNSSVEQERKIKENELNTEIAIENK
                     KRQIKETQMEAEKSVQQKKHELQQSEMLFSIQQEDEKKNLVSIAVQNARAEADASAYA
                     ISAMMKAFEGVDTGVIQALASTGMNPQQLIASAFQGLAEKADKIGQLNISPELLNEIL
                     KKSDRK"
     gene            2519..3457
                     /locus_tag="P5123_10470"
     CDS             2519..3457
                     /locus_tag="P5123_10470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013239078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar kinase"
                     /protein_id="HRX16727.1"
                     /translation="MEPLSENKIVIITRKTRLDDLIARFNSVSQAKFYVEHLGADFSD
                     YEKEHNEYILAVKQASELLSAIGRIHVLDREYLTNYIFGPNDVVVAIGQDGMVANTLK
                     YLSGQSLLGVNPSPDRWDGVLLPFYVEDLPLVLPEVIRGQRGHKEVTIAAARLNDNQE
                     LLAVNDFFIGQKSHVSSRYKIEYAGLTEAHSSSGIIVSTGLGSTGWFKSILAGAQGVV
                     GKISGNDFNIESNDDFSWDSEYLYYSVREPFPSNNTGTELVFGKITKQQPLKITSQMS
                     ENGVIFSDGIEKDFLEFNSGMEAVIGLSERKGFLVV"
     gene            3620..4771
                     /locus_tag="P5123_10475"
     CDS             3620..4771
                     /locus_tag="P5123_10475"
                     /EC_number="6.5.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013220762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008452 - RNA ligase activity [Evidence
                     IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RtcB family protein"
                     /protein_id="HRX16728.1"
                     /translation="MINITTEKVPIKMWLDEIEDGAMQQAKNLANLPFVFKWIALMPD
                     CHQGYGMPIGGVLAAYQVVVPNAVGVDIGCGMCAVKTSLKNINSDQLKKIISLIRARI
                     PLGFNHHKHPQQWQGFDNAPDIPIIQQELQSAQYQLGTLGGGNHFIEMQKGSDGFIWI
                     MLHSGSRNFGLKIATEYHKKAMYYCAQNKYQLTDSNLAYLPVKTEEAKEYFEAMNFAL
                     EFARESRLKMLEVVKDAVASVCINVSFDETINIHHNYAALERHYGHDVIVHRKGATSA
                     RKGQIGIIPGSQGTRSYIVIGKGNPESFMSCSHGAGRKIGRKEAQRKLDLKHEKKILD
                     DQRIIHSIRTVKDLDEASGAYKDIDTVMKNQQDLIDITVSLVPLAVIKG"
     gene            complement(4784..5686)
                     /locus_tag="P5123_10480"
     CDS             complement(4784..5686)
                     /locus_tag="P5123_10480"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRX16729.1"
                     /translation="MKETDNEYSAFGPWIYEIDDSHPLPHALKKYTDKTSNYKMLIKI
                     PVNIERRKLKPGMDMYNRVLGVHNDYFQIISKHGDKVNSEKVHYDKIEAIEYKFDLLE
                     GKFTLFTDKSSFETIFNASSMETISKMAKLVRDTIIKEQAPIDIAQYKTVSTDTSLFF
                     KNIYDELKKENEDFKIIGYHDDIDIRYAAYKLHRFLFQMLLKPKIAGTLHVANGKELA
                     VITHGSHTVKTVKDPAIAGSFLFIPFKSIRQIHKTVSDKYNGIIKYKIVLNNHSFNFY
                     RNEAVQLNYLDKLEAQLRDINSGK"
BASE COUNT         1954 a         1006 c         1218 g         1862 t
ORIGIN      
        1 gaccctgcag tattgtgata atattcaaat tgtaatttta ataagaggat aatgatattg
       61 aataaatttg caaaattact tattgctttt tgcccactgc ttgtattttt gattttgtta
      121 gtgttatatt ttaaatatgt tgaagagata ttatattact atttcgattg tggatgtttt
      181 actcatggca ttaactataa tgtcgcatca attattgttt tatcattagt aaattttttg
      241 tatattgttc tctttgttcg atatgcaggg tgtctaagaa ataaatccaa aatagtgtat
      301 ttgttggtaa atgttttgat atccactctt tttttagtta tagtggttta taactaaaac
      361 acatcgtgag taattatata tattaagttt tcatatttgg atagcatttt aaaaaaatgg
      421 tatttctaat caatgatcag gggtgacaag caagtggtta ctatacaaac agagcgtctt
      481 ataatccgtg ataatcttaa aaaagattta aatgatcttt acgcgctaac gtcagatcct
      541 gttgccatgc ggtttatgaa aagcacaaaa attgaatcca ttagcgaagc aaagaaaaac
      601 ctaaaacaat ccataaacga atccaaaaaa aagaatcgtg aaaaatacta ttttgccatg
      661 attaacagaa gtacaaatga atacatcggt gcaataggct atatcgtaga agctgtatat
      721 ccgcaaggta aacttgtcga gctcggttat tttatcaaac agcagtattg gcgaatgggg
      781 tatacaactg aggctggatc ggctgttatc aaatttgctt ttgaaaatga taatgtaata
      841 aagattgttg cggcatgctt aaaagaaaac tgcgcatccg agaataccat gataaagctt
      901 ggatttcgaa aagagggaga gcttaataat tatcagtttc atgaaaacat ctggaaaacc
      961 cgtgttttct atggattgct aaaagaggat taccttgcag gctgactttt acccatgttt
     1021 attatcttgc aaatgtctga tactaagccc cctaaatccc ccaaaggtgg acttttaata
     1081 gttattgcac agaactgtat ttataataaa atatagatat gctaaataat aaaataaaag
     1141 taaattttac ctaaatgtgt ttttgaattt gcaagacaaa acagataaaa tctagctgtt
     1201 tctgagcaat aaacatggtc acaatctgca ttattgggaa aagatgaatt agaagccccc
     1261 tttgggggtt ggggcatata gattaaaatt tttgtgctgg aaatttattg acagttattt
     1321 aaaagtaaac taattcataa tttatatgcc gtgattagtt acaaacaggt tagaatctgc
     1381 tgtttgccaa agcctttagc tatagtataa attgcttggg tacaaatgta taaataaata
     1441 acttcgatac aaaatataga ttcgagatat attacaagga ggattaaatg tttggtttta
     1501 aatttatcaa gtttcaaccg acggattacg tactgagata taaaaacgga aagatagtca
     1561 aagaggggtt ggggctctct tttttctatt atgcccccag aacatcgctg gtgtcggttc
     1621 cgattggaag cgccgaggtg ccctttatat ttgaagaggt tactaccgat tatcaggcga
     1681 taaccattca gggaacagta acctacaaaa tagcagagcc aaaaaaaata gccgaatacc
     1741 tgaattttac tctggactat accggtagct cttatctgtc cgatgacccg caaaaactgc
     1801 cacagcggat tatcaatgct gtacatgttc ttaccaaaaa agagatgcaa aacctctctc
     1861 taagagaagc aataagatca tcggaagctc tagtcgcgtc ggtttcacaa ggacttggca
     1921 aaagtaccga aataacttca ttgggtatca aggttcttgg aatatccatt ttggctataa
     1981 agcccaataa agagacagcg cgcgcgcttg aggccgaagc ccgtgagcag attttaaaag
     2041 aggcggatga cgcgatctat acaagacgta actcgtccgt agagcaggag cgcaagataa
     2101 aggaaaacga gctaaataca gaaatagcga tcgaaaataa aaagcgtcag atcaaagaga
     2161 cccagatgga agccgaaaaa tcggttcagc aaaaaaaaca tgagctgcaa cagtcagaga
     2221 tgctctttag tatccagcaa gaggatgaaa agaagaacct tgtttcaatt gctgtacaaa
     2281 acgcgcgtgc agaggccgat gcgagtgcgt atgccatttc ggcaatgatg aaagcttttg
     2341 aaggggtaga taccggagtt atacaggcgc ttgcgtcaac cggaatgaac cctcaacagt
     2401 taatagcttc tgcgtttcag ggtttagccg aaaaagcgga caaaattggc cagctcaata
     2461 tttcgccaga gcttttaaat gaaatactaa aaaaaagtga tcgaaaataa tcaaatcaat
     2521 ggaaccttta agtgaaaata agattgttat cattacccga aaaaccagac ttgatgattt
     2581 aattgcgcgt tttaattcgg tttctcaggc aaaattttat gttgaacatc ttggtgccga
     2641 tttttctgat tacgagaaag agcataatga gtatatcctt gcagtaaagc aagcgtccga
     2701 acttctttcg gctattggac gtatacatgt tcttgaccgc gagtacctta ccaattatat
     2761 atttgggcct aatgatgtag tggttgccat tggtcaggat ggtatggttg ctaacacatt
     2821 aaagtatttg tccggtcagt cgcttttggg tgtcaaccca tcgcctgaca ggtgggatgg
     2881 tgttttgttg ccgttttatg tcgaagattt accgcttgtt ttgcccgaag ttatacgtgg
     2941 tcaaaggggg cacaaagagg taacaatcgc ggcagccaga ttaaacgata atcaggagtt
     3001 gcttgctgtt aatgatttct ttatcggaca aaagagtcat gtatcttctc gttacaagat
     3061 tgagtacgcg ggtcttaccg aggctcattc atccagtggt ataatagtct caacaggtct
     3121 aggttctact ggatggttca aaagtattct ggctggagca cagggtgttg tcggaaaaat
     3181 ctccggaaac gattttaata ttgaatctaa cgacgatttt tcatgggatt ccgaatatct
     3241 ctattactcg gtaagagagc cgtttcccag taataatacc ggaacagaac ttgtctttgg
     3301 aaaaattaca aaacagcagc cgctaaagat aacttcacag atgtcagaaa acggagtaat
     3361 attcagtgat ggcatcgaaa aagatttttt ggaattcaat tcgggaatgg aagccgtaat
     3421 aggattatcc gaaagaaaag gatttctggt tgtttgacga tgataagtta cagtgcgctc
     3481 aattacccaa gtgaactttt attttttgaa gtaatccgat tagtattatt aaacgagttt
     3541 cccattaaaa acaacagata ctaaatatcg gtaatcttta ataattaact ttttttcgat
     3601 gatctctgtc aggttataaa tgataaacat tactactgaa aaagttccca taaaaatgtg
     3661 gcttgatgaa atagaagatg gagccatgca acaggcaaaa aatctcgcta atttgccttt
     3721 tgtatttaag tggattgccc taatgcctga ctgtcatcag gggtatggca tgcctatcgg
     3781 tggagttctt gcagcctatc aagttgttgt gcctaacgct gttggtgtag atatcggatg
     3841 tggaatgtgt gctgtcaaga catccctgaa aaatattaat tctgatcaat taaaaaagat
     3901 aataagcctt attagagccc gaataccgct tggatttaat catcataaac atccgcagca
     3961 atggcagggt tttgataatg caccggatat accgattata caacaagagc tacaaagtgc
     4021 acagtatcag ttgggaacac ttggcggtgg taaccatttc atagagatgc aaaaagggtc
     4081 tgatggcttt atctggataa tgcttcattc tggatctcga aactttggtc taaaaattgc
     4141 caccgagtat cataaaaaag ctatgtatta ttgcgctcaa aataaatatc aacttacaga
     4201 tagtaatctg gcttatcttc cagtaaaaac agaagaggcc aaagagtatt ttgaagcgat
     4261 gaactttgct cttgaatttg caagggaaag ccgcctaaaa atgcttgaag tggtgaaaga
     4321 tgctgtagca tcagtttgca taaacgtatc ttttgacgaa actattaata ttcaccataa
     4381 ctatgctgcc ttggaaaggc actatgggca tgacgtcatt gttcacagga agggggcaac
     4441 ttctgcgaga aaggggcaga ttggaataat tcctggttcc cagggaacca gaagctatat
     4501 agtcattggt aagggtaatc cagaatcatt tatgtcttgt tctcatgggg cgggtcgcaa
     4561 aattgggcgt aaagaggctc aacgcaaact tgatttgaaa catgaaaaaa aaattcttga
     4621 tgatcaaagg atcatacatt caataagaac tgtcaaagat cttgacgaag cgtcgggtgc
     4681 gtacaaggat attgatactg taatgaaaaa ccagcaagat ctaattgata ttaccgtcag
     4741 ccttgtacct cttgcagtga taaaggggta gatgctcaag cactcacttt ccagagttaa
     4801 tatccctcaa ctgtgcctcc agcttgtcca gataattaag ttgaaccgct tcgtttctgt
     4861 aaaaattaaa actgtgattg ttaagcacaa ttttatattt aattatacca ttgtatttat
     4921 cagaaactgt tttgtgtatc tgtctaatgc tcttgaaagg aataaacaga aagctgcccg
     4981 ctattgcagg atccttgact gtcttgacgg tatggctacc atgagtgatt actgcaagtt
     5041 ctttgccatt tgcaacatga agggttcccg ctattttcgg tttcaaaagc atttgaaata
     5101 aaaaccgatg caatttgtac gcagcatatc gtatatcgat atcgtcatga taaccgatta
     5161 ttttaaaatc ttcgttctct ttttttagtt catcatagat gtttttgaaa aacaggcttg
     5221 tatctgtcga aacagttttg tattgagcga tatcaatcgg tgcttgttct tttatgattg
     5281 tatctcgaac caatttggcc atcttgctta tggtttccat ggacgaggca ttaaatatag
     5341 tctcaaacga tgatttatca gtaaataatg tgaattttcc ttctagcaaa tcgaacttat
     5401 attctatcgc ctcaattttg tcataatgaa ccttttccga attaacttta tcaccatgct
     5461 tgctgataat ttggaagtaa tcgttatgaa cacccaaaac cctgttgtac atgtccattc
     5521 cgggtttaag ctttcgtctt tcaatattaa caggtatttt tataagcatt ttgtagttgc
     5581 tggttttatc cgtgtatttt tttaatgcat gtggcagtgg atggctatcg tcaatttcat
     5641 agatccaagg cccgaaagcg ctatattcgt tatctgtctc tttcataata tcctccgtta
     5701 cgggatgatc gtgtgggagt aaattaggtg catattgtgg gtttaaaaag aaggcaagct
     5761 agtcccgata gtgttatata ataaaataca tattttccat ggtaaatcaa ataaagttaa
     5821 ataatttatt ggttaagcag ctaaaaaaaa tccaaataat cgttaataat caagtttgct
     5881 tattgacacg atttcaggac ccatagtatc agaaataaat tataagtagt gaatttgacg
     5941 attacttata taaaaaatat aaggataatt attggtttta ttgcggagag cgttttaatt
     6001 gaatctcttt ggttactgat agttgtctga taaaacagcc
//
LOCUS       DAOTGZ010000173         6026 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_3064
            NODE_15561_length_6026_cov_4.710434, whole genome shotgun sequence.
ACCESSION   DAOTGZ010000173 DAOTGZ010000000
VERSION     DAOTGZ010000173.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868088
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 6026)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6026)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:22:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,785
            CDSs (total)                      :: 2,751
            Genes (coding)                    :: 2,732
            CDSs (with protein)               :: 2,732
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 12 of 19
            Pseudo Genes (internal stop)      :: 5 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6026
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15561_length_6026_cov_4.710434"
                     /isolate="AMR_MDS_3064"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..901)
                     /locus_tag="P5123_10485"
     CDS             complement(<1..901)
                     /locus_tag="P5123_10485"
                     /inference="COORDINATES: protein motif:HMM:NF024242.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HRX16730.1"
                     /translation="MISGQKEYINEKETKDHEFEIIQNLVGNASIEQLKNVDYAVIDN
                     FGIFVPSIGPCLYSISPAHTHPSYMIIFNMFNYCNVKMNDQILSVSPYTVSVFSPDIE
                     HQEIIDEDYNRYYAIMIEKTLFEKYLKNFTNHRPSFIGKNFKPDDNYKFYINEFICEF
                     SKNIRNMQQLHLIAERISYLTVSGLLDHTTNQNSEFYQSGMDNAIEFMYHNYSENITV
                     DAISSFCGFSSSYFSRLFKSCTGLSPMTYLKQIRLKKARKLLISSNKSITEIAFLCGF
                     NSSAYFTAAFSEAFETSPTSLRKN"
     gene            complement(1264..1638)
                     /locus_tag="P5123_10490"
     CDS             complement(1264..1638)
                     /locus_tag="P5123_10490"
                     /inference="COORDINATES: protein motif:HMM:TIGR00621.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HRX16731.1"
                     /translation="MSNLSNVTIEGFVTQEPLLKKTKTGKDVCNFSLAINHNSEKGEE
                     AKVSFIDIETWEKTALSCAERVHKGKRVIVMGNIRQDRWQAEDGSPRSKIKVVGNQVR
                     YLTAKKDEELLEKSTQQKAFAV"
     gene            complement(1760..4102)
                     /locus_tag="P5123_10495"
     CDS             complement(1760..4102)
                     /locus_tag="P5123_10495"
                     /inference="COORDINATES: protein motif:HMM:NF024799.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="substrate-binding domain-containing protein"
                     /protein_id="HRX16732.1"
                     /translation="MEIKKRPTIAILVDYTDTEFQRGILAGIDHEARARDINLLIIEG
                     GLAKSDCIIKTRQNIIYNLAKSRKIDALILFSASIGMYLNDEEFESFIKQFEKIPVIS
                     LSREIQGISSILVNNNIGMIDMMDHLVKVHKYSKFLYMRGAVGNYDDEERFSLFSSYL
                     KKNRIPFDDDSVLVGDFILGNAMDSFEGYLKNHKLDHDVIVCANDEMAVGIVRVLNQK
                     GFKVPDDVAIVGFDNFLNSRYLTPPLSTVAQPLFKLGQNAIESASMQILTGKNSPTIY
                     LPSSFIHRESCGCGGIKTINSVPDFSLNSPLDHDTFRNHLINKLQKSIEHSLSVILVK
                     HSHSEITTTAHEFVMSFVKAVFEKKPEVFLDYWSDFLETAKDYEQDYTILRDLIFLIA
                     STASQRICNHDDLFLIQEIWKWATDYLAKKCVLEEKHANLDHDIDGRIIDEIRFNLIS
                     STNLDEYLSLVQKAISHLGIQHCFATLFKEKTVSKTLPEKSTLLFAFENGNSNKVKSG
                     EFDTLDILPEGCLPKNKRFCIHAELLSSSNQRIGLLCADFKIEKIRSLRSFRQVLSNS
                     LFSRNLIIKVNQQQLDLEKNIAIIRRTMGGFIQTLQQTVELRDPYTAGHQRRVSELAR
                     MIAQEMGLSKQIIETVRIGGVIHDLGKINVPVEILNKPGKLRDVEITLLRTHPEIAYE
                     ILQPIDFPWAIADVVVQHHERLDGTGYPKGLTGDNIMLEAKIISVADVVEAMASHRPY
                     RPALGLDTALNEILSKRNSWFDADIVDTCIFLLQSGKYKL"
     gene            4384..5436
                     /locus_tag="P5123_10500"
     CDS             4384..5436
                     /locus_tag="P5123_10500"
                     /inference="COORDINATES: protein motif:HMM:NF012981.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016301 - kinase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase family protein"
                     /protein_id="HRX16733.1"
                     /translation="MKSIYRAIIHSVCQSVWVKEKEYFPHIVINPYAGTIKSRVKRRY
                     VENVISETFGISEYPLDKYEYQLYVTEFAQHGYEISKQIIDYIKHEKRKKHLIVIAAG
                     DGTAHELMSVFVKHEKILKNVLFFRLPFGTGNDASDCENIYQALQIQAGFGMDYPQNI
                     LKVSTAKGRVFYSFNIASLGLDGYVVKMTNRLKKIIPGSFYTLFVDIATLLYEKLYPP
                     QMMQITVTNGKESVKYDGIYLFGAMGVSGYRTYGDHKKILPDRNNICFCRPIPLLQKI
                     MKKSYFFDGRHIMLKNVSMAKGNKMVIDYNHSIPFQTDGEDYLFTREDFPVIFEIPDI
                     RLKVLKHHQPDINNRN"
     gene            5452..>6026
                     /locus_tag="P5123_10505"
     CDS             5452..>6026
                     /locus_tag="P5123_10505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018931674.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRX16734.1"
                     /translation="MIKNRCRFAVRVIITSMFFPLFLYANTLLASDDETEAFKTVPLN
                     ILEDQEVCLKEFGVTVNLPKNNRITGTVRLHGKENPLRGIDIVYTDINGFEHTLKTNR
                     DGRFTLFNIAHPYTVRALEKDYILRSASLDMEKGDSIVHLNLVMNKRANVIAMIFSVI
                     GGLGIFLLGMRYMSEGLQSVSGPTLKRLISL"
BASE COUNT         1843 a         1082 c         1134 g         1967 t
ORIGIN      
        1 gattcttgcg taaagaggtt ggtgatgttt caaatgcttc tgaaaatgcc gcggtaaagt
       61 aggccgagct gttgaaaccg cacaaaaaag ctatttctgt tatactttta tttgagctta
      121 tcaaaagttt acgagctttt tttagcctta tctgctttaa ataagtcatt ggacttaatc
      181 ctgtgcacga tttaaagagc cgtgaaaagt atgaagacga aaatccgcaa aatgatgata
      241 tagcatcaac tgtgatattt tcactgtaat tgtgatacat aaattcaatc gcattatcca
      301 tcccagattg gtaaaattcg ctgttttgat ttgttgtatg gtctaacagg ccagacactg
      361 taagatatga tattcgctcg gctatcaggt gtaattgttg catgttacga atattttttg
      421 aaaattcaca aatgaattca ttgatataga acttgtagtt gtcatccggt ttaaaatttt
      481 taccaataaa tgaaggtcta tggtttgtga aatttttcag atatttttca aataacgttt
      541 tctcgatcat aatagcgtaa taacggttat aatcttcatc gatgatttct tgatgttcta
      601 tatcgggtga gaatacagat actgtatatg gtgaaacaga aagtatctga tcattcattt
      661 tgacattgca ataattaaac atattgaaga ttatcatata cgacgggtga gtatgtgcag
      721 gagatatgga gtacaggcat ggaccgattg atggcacaaa aataccgaaa ttgtcaataa
      781 ccgcataatc tacattcttt agttgttcta ttgaggcatt gcccaccaaa ttttgaataa
      841 tttcaaattc atggtctttt gtttcttttt cgttaatgta ttctttttgg ccacttatca
      901 tggttatctc tgataacaat tttatatttg tgtttagtgt aatatgtaat ggtcgagcag
      961 acaatatcaa cttatttgcc ggtgcttttc ggccggctca ggtacttttg aaaattctgt
     1021 taaaacagac aagtgaatac tgataatcat aaaattagca attcttgaaa gttctcttca
     1081 gtgaaaaatg ccgtaaagtt ttaattataa gtcgtgtgaa attatattct aaaaaaatgt
     1141 tatgcatttt aaagtcaata aatgtttaaa tgtgttacgc taaaattatt agttattggt
     1201 aaatttttaa gattacttac cgttatgctt aaaaaaaacg ggcgcatttg cgcccgtttt
     1261 tgatcagact gcaaaggctt tctgttgtgt acttttttcc agcaattctt cgtctttttt
     1321 tgcggtaagg taacggacct gattgccgac aacctttatt ttggaacggg gggaaccgtc
     1381 ttcggcctgc cacctgtcct gtctgatgtt tcccatcaca atcacgcgct tgcccttgtg
     1441 aactctctcg gcacaagata gagcagtttt ttcccaggtt tcaatgtcga taaaagaaac
     1501 tttggcttct tctccttttt cggaattgtg gttgatggct aatgaaaaat tgcaaacatc
     1561 tttgccggtc ttggtctttt tcaatagcgg ttcctgtgtt acgaaacctt cgattgtaac
     1621 gtttgataaa tttgacatac acaccccttt cccgtttcct tgttgaaacg gataaaaaat
     1681 tttttaactt cctgtctatt acaacgaaga aatcagaaaa aagggcgaat ttttttaaaa
     1741 aaaatgcaaa aaaaatttgt tataatttat atttaccact ttgaagaaga aaaatgcatg
     1801 tatcaacgat atcagcatca aaccaactat ttcttttgct caatatttcg ttaagggctg
     1861 tatcaagccc taaggcgggt cggtaaggtc tatgcgaggc catggcttcc accacatctg
     1921 cgacactgat gatcttggcc tccagcataa tattatcgcc ggtcaaacct ttagggtatc
     1981 ctgttccatc gagccgctca tggtgctgta caaccacatc ggcaatagcc cagggaaaat
     2041 caatgggctg tagtatctcg taagctattt ccggatgtgt gcgtaacagt gtaatttcga
     2101 catcgcgtag ttttccgggt ttgttaagaa tttcaacagg aacattgatt ttacctaggt
     2161 cgtgtataac tccaccaatt cgtaccgttt caataatctg ttttgagagt cccatctcct
     2221 gagcgatcat ccttgccagc tcggaaaccc gacgttgatg tccagcagtg taggggtcac
     2281 gaagctctac ggtttgttga agtgtttgta taaaaccacc cattgtgcgt cttattatgg
     2341 caatgttttt ttccagatca agttgttgtt gattaacctt tataatcaaa tttcgtgaaa
     2401 aaaggctatt gctaagaact tgtctgaagc ttctaaggct tctgatcttt tcaatcttga
     2461 aatcggcaca tagtaatccg attcgctggt tcgaggagga gagaagctcg gcatgtatgc
     2521 aaaatcgttt attcttaggt aaacagcctt cgggcagtat gtccaaagtg tcaaattcac
     2581 ccgatttaac cttgtttgaa ttaccgtttt cgaaagcaaa aagaagtgta gacttttcgg
     2641 gaagtgtttt gcttaccgtt ttttctttaa acagcgtggc aaagcagtgt tggatgccaa
     2701 gatgagatat agccttttga accagtgaaa gatactcatc aagattcgtt gatgatatca
     2761 gattgaatct aatctcatca ataattcggc catcaatatc atgatcaaga ttggcgtgtt
     2821 tttcttccaa cacacatttt tttgcaagat aatcggttgc ccatttccat atttcctgta
     2881 tcagaaaaag atcgtcatgg ttgcatattc tttgagaggc agtagaggca atcagaaata
     2941 taagatctcg taaaattgtg tagtcttgct cataatcttt tgctgtttcc agaaaatcgg
     3001 accaataatc cagaaaaacc tcaggttttt tttcaaatac tgctttaaca aaagacataa
     3061 caaattcatg agcagtagtg gtgatttccg agtgtgaatg tttgaccaat attaccgaca
     3121 aggagtgctc tatcgatttt tgaagcttat ttattagatg gttcctgaac gtgtcatggt
     3181 caagaggtga gttcaaagaa aaatcgggaa cactgtttat agtttttatt cccccacagc
     3241 cgcatgattc acggtgtata aacgagcttg gtaaataaat tgttggtgaa tttttacccg
     3301 ttaatatctg catgctagcg ctctcgatag cgttttgacc caatttgaat agaggctgtg
     3361 ccacggtcga aagcggtggt gtaagatagc ggctgtttaa aaaattgtca aaaccaacaa
     3421 tcgcaacatc atctggtact ttgaatcctt tctggtttaa aaccctgact atgcctacag
     3481 ccatctcgtc gttggcgcat acaattacat catgatcaag tttgtgattt ttaaggtagc
     3541 cctcaaaaga atccatggcg tttccaagta taaaatcacc tactaataca gaatcatcat
     3601 caaagggaat acgatttttt ttaagatacg aagaaaacag tgaaaaacgt tcttcatcat
     3661 cataattacc aaccgctccc cgcatgtaca aaaatttgct gtatttatga accttcacca
     3721 gatgatccat catgtcaatc atgccaatat tattgttcac aagtatggaa gagattccct
     3781 gtatttcacg tgacaagctt attaccggaa ttttctcgaa ctgttttata aaactttcaa
     3841 attcttcatc attaaggtac atacctattg aagcagaaaa gagtattaag gcgtctattt
     3901 ttcttgattt tgcgagattg tagatgatat tttgccgggt tttgataata cagtctgatt
     3961 tagccaatcc gccttcgatt atcagaagat ttatatcgcg tgctcgtgcc tcgtggtcta
     4021 ttcccgcgag aattcctctt tgaaattctg tatctgtata atctacgaga attgctattg
     4081 tgggtctttt tttgatttcc atcgcaaatc ctgtaagcgg tttaatacaa ttataaaaaa
     4141 acgtaaacag tttactctaa aatatatcag ttacacacgt aactataagt ttgttcaagt
     4201 ttgttaaaaa tcaagatttg tatacatata agtatcttat acctcataat tttgtattta
     4261 acaacaaaaa gctcctttag tctgttttgc cggtattttt aacagaaaac aagtgaaaaa
     4321 agtattggaa tattttcagg taaataggat gatgtaatcc gattaacaag caggtgactt
     4381 taaatgaaat caatatatcg tgcgataatt cactcggttt gccaatcagt atgggttaaa
     4441 gaaaaagagt attttcccca tattgtaata aatccttatg caggcacaat caagagccgc
     4501 gttaaaagaa gatatgtcga aaatgttatt agtgaaacat tcggaataag tgagtatccc
     4561 cttgataaat acgagtatca gctatatgta accgaattcg cccagcatgg atacgaaatt
     4621 tccaaacaga ttatcgatta tataaaacac gaaaaacgaa aaaagcatct tattgttatc
     4681 gccgcaggtg acggaactgc tcatgagttg atgagtgttt tcgtcaaaca cgaaaaaata
     4741 ttaaaaaatg tacttttttt tcggctccct tttggaactg gcaatgatgc gtcagattgt
     4801 gaaaatatct atcaagctct gcagatacag gccggttttg gcatggacta tcctcaaaac
     4861 attctcaaag tatcaacggc aaaaggtagg gtattctatt catttaacat agccagcttg
     4921 gggcttgatg gatatgttgt aaaaatgaca aatcgcctaa aaaaaataat acctggatct
     4981 ttttatactc tgtttgtgga catagcaact ctgctttacg aaaagctata ccccccgcaa
     5041 atgatgcaaa taaccgtaac gaatggtaaa gagtctgtaa aatatgatgg aatatatctt
     5101 tttggagcaa tgggtgtctc aggatacaga acctatggag accataaaaa aatattgcct
     5161 gatcgtaata atatctgttt ttgccgtccg attcccttat tacaaaaaat catgaaaaaa
     5221 agttattttt ttgacggaag acacatcatg ttaaaaaatg tctccatggc taagggaaac
     5281 aagatggtaa ttgattacaa tcattcaata ccgtttcaaa cagatggaga ggattatctt
     5341 tttacccgtg aggattttcc ggtaattttt gaaataccgg atatccggtt aaaggtgctt
     5401 aaacatcatc agccagacat aaataaccgt aattaactat aaggtgttaa catgataaag
     5461 aatagatgcc gatttgcagt tcgcgttata attacttcta tgtttttccc ccttttttta
     5521 tatgctaata cgcttctcgc ttcagatgat gaaacagaag catttaagac tgtacctctg
     5581 aatattctcg aagatcagga agtatgtctt aaagagttcg gggttactgt taatctcccc
     5641 aaaaacaaca gaataacggg tactgtccgc ttgcatggta aagagaaccc gcttcgtggt
     5701 atcgatatag tctatactga tataaacggt ttcgaacaca ccctgaaaac aaatagggat
     5761 ggtcgtttta ccctttttaa tattgcccat ccatatacgg tacgcgcttt agaaaaggac
     5821 tatattttgc gcagtgcctc tctggatatg gagaagggag attcaatagt tcatcttaac
     5881 cttgttatga ataaacgcgc caatgtaatc gcgatgattt tcagtgttat aggtggcctt
     5941 ggaatatttc tgcttggcat gcgttacatg tccgaggggc ttcaatctgt ttcaggtcca
     6001 accttaaaaa gacttatcag cctgat
//
LOCUS       DAOTGZ010000174         5836 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_3064
            NODE_16240_length_5836_cov_5.657326, whole genome shotgun sequence.
ACCESSION   DAOTGZ010000174 DAOTGZ010000000
VERSION     DAOTGZ010000174.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868088
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 5836)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5836)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:22:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,785
            CDSs (total)                      :: 2,751
            Genes (coding)                    :: 2,732
            CDSs (with protein)               :: 2,732
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 12 of 19
            Pseudo Genes (internal stop)      :: 5 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5836
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16240_length_5836_cov_5.657326"
                     /isolate="AMR_MDS_3064"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..593)
                     /locus_tag="P5123_10510"
     CDS             complement(<1..593)
                     /locus_tag="P5123_10510"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRX16735.1"
                     /translation="MKLHNTKRFSVYKAVITMLCLVAAFKTNAIAQQQTDSEYDDLFS
                     GMDQSSDSIIEPDKMTFSAMHCYTMPVLTGKNEYGYQPLHISPVTHKLQLKIDDKNIT
                     ALTGVQIYNSTDSDALKDAELAYNQTYIRYSMSGLNITAGNIIYSWGSADSLNPTDRL
                     NPRDERNPYDMTKVPLTSLMVSLFTSDSFSLDFILIPG"
     gene            complement(596..1363)
                     /locus_tag="P5123_10515"
     CDS             complement(596..1363)
                     /locus_tag="P5123_10515"
                     /inference="COORDINATES: protein motif:HMM:NF028441.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_process: GO:0042953 - lipoprotein transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane lipoprotein-sorting protein"
                     /protein_id="HRX16736.1"
                     /translation="MLRFISLFFLVILCSVQNSFCDDKAFAIMKNHFNLKSAPCYTAR
                     ISLMLIDGKGKKLSRSFNQQNIDRDSFEANYIEVTDPADIAGVRLLSVNTNNRDEQRL
                     YLPALAKSRLISGSGNSGKEGRFLGSDINYYDLENKRLDDAVYSYVSEEIIAGTSCHI
                     IDAAPKDKDCPYSKTRFWVDANSWLIHKIQAFDSKNNLIKEIQNRSYTTINDIHFVKE
                     MVIRDIRRNHTTLYKLNTIDIKTRVSPSVFTVQYLER"
     gene            complement(1553..1987)
                     /locus_tag="P5123_10520"
     CDS             complement(1553..1987)
                     /locus_tag="P5123_10520"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRX16737.1"
                     /translation="MYKKSNSKKAIIMIIKDFLVILPVSAFIGIAVGTPVTLYLKDPL
                     LPGIVRSVLTALIIGFFATLAFAWLFRTIRKTPLVAFVAEFIIIAAGSYIGASINGIN
                     SLLTHLFIIFFAELFGIGFGMYMYKKTVRLNSLLKHKQQKLC"
     gene            complement(1980..2516)
                     /locus_tag="P5123_10525"
     CDS             complement(1980..2516)
                     /locus_tag="P5123_10525"
                     /inference="COORDINATES: protein motif:HMM:NF016296.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LytTR family DNA-binding domain-containing
                     protein"
                     /protein_id="HRX16738.1"
                     /translation="MTKFAIIANNKEELLKAGIVESSTSSIALVEEGFTIPNDKEICI
                     VYKANSLPQLIEMILYTGRMASNEVDTISCYDDERIMLVPIDEIEFITADRNRIECYI
                     KKGQILQIKKKIYELEYMLSSKGFFRVNKSTIVNVREIKDISPWFGGRLLLRMKNGTD
                     EIEVSRKYSADFKEYLGV"
     gene            2914..3705
                     /locus_tag="P5123_10530"
     CDS             2914..3705
                     /locus_tag="P5123_10530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016402054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="family 16 glycosylhydrolase"
                     /protein_id="HRX16739.1"
                     /translation="MKKLLSVLFLAVVLIACSSKDDEVKFYYGAEVFSHKPVLFGKFE
                     VRAKLINESGVVSSFFLYDNASWQGRPFPWREIDIESIGKEKGMLQTNIITGFADKRV
                     TSEGETVIENVGTEYKTYTIEWTPDYVAWFVDGKEIRRDTAESSQQVVDLRDTPQTYR
                     MNVWIAIWDEWVGKFKESSFPMYQSINWIKYYEYTPEKSEKFTHKWTDDFDTFDESRW
                     GKGDWSFEGNLVVFVKGNAEIKDGHLVLGLTKDEFGYSGEFLKDE"
     gene            complement(4168..5043)
                     /locus_tag="P5123_10535"
     CDS             complement(4168..5043)
                     /locus_tag="P5123_10535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020775471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyl transferase AbiEii/AbiGii toxin
                     family protein"
                     /protein_id="HRX16740.1"
                     /translation="MHSAVESMLEKYNCKSNFDYIHALKEIIQEITLLGLYRSGFYSK
                     AAFYGETALRIFHGLERFSEDLDFTLTKKNENFDFSSFLKFVKAELESFGFELDIETK
                     TKLKNTNIKSAFIKGGTLIHLLKINSVTPPVSGVHPDEKIKVKFEIDIDPPEGATTEM
                     KYHLIPIPFTVQLYTLPSLFSGKLHAVLCRNWQNRIKGRDLYDLVWYISKGAKVNLNH
                     LNKRMIQTGHLQIDDTFNNTTLVNKLMERICSIDFNQAKLDVLPFIKNPDSVELWSKD
                     FFISIIKDHAFIPSP"
     gene            complement(5043..5636)
                     /locus_tag="P5123_10540"
     CDS             complement(5043..5636)
                     /locus_tag="P5123_10540"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRX16741.1"
                     /translation="MIIDSTYLLKKLENYSSPKTKISRMAQKNDILKIRRGLYIDKSE
                     GFSSVQVIANLLYGPSYISFEYALSYYGLIPEKTVSVTSAVYNKNKRKTYKTDVGYFY
                     YYCIPPAVYPYGIIIEKLENDYFMISTPEKALCDLLYRCKNFNTTHQIEQFLFEDMRL
                     EEDFINKIESSKISFLAPLYNNKNMYLFDTYLQEKER"
BASE COUNT         1742 a          947 c         1240 g         1907 t
ORIGIN      
        1 ccgggtatca gtataaagtc cagagaaaag ctgtcagaag taaagagcga aaccataagt
       61 gaagtgagtg gaacctttgt catatcgtat ggattgcgct cgtcacgcgg gttcagccgg
      121 tcggttgggt taagcgaatc cgcgcttccc caggagtaaa ttatattgcc ggctgtaatg
      181 ttcaggccgg acatactgta acggatatac gtctggttat aggcgagttc tgcatccttc
      241 aaagcatcac tatctgtcga attatagatc tgcactcctg ttaatgcagt gatgtttttg
      301 tcgtctattt tcagttgcag cttgtgtgtg actggtgaaa tatgcaacgg ctgatatccg
      361 tattcgtttt ttccggtcaa gactggcatg gtgtagcagt gcatagctga aaaggtcatt
      421 ttgtcaggtt ctattatgct gtcagaagat tgatccatac ccgaaaaaag atcatcatat
      481 tcggaatcag tttgctgctg tgctatggca tttgttttaa aagctgcaac aagacagagc
      541 attgtaataa cagctttata aacagaaaat cgttttgtgt tatgaagttt cattgttatc
      601 tttccagata ctgtactgta aatactgagg gtgaaacccg ggttttgata tcaatagtgt
      661 tcagtttgta gagtgttgtg tgattgcgtc tgatatccct gataaccatc tctttcacaa
      721 agtgaatatc gttaatggtt gtatatgagc ggttttgtat ctctttgatg aggttgttct
      781 ttgagtcaaa cgcttgaatt ttgtggatca accagctgtt tgcatctacc cagaaacggg
      841 ttttggagta tgggcaatct ttgtcttttg gagcggcatc aattatatga caagaggttc
      901 ccgctataat ctcttcgctg acatacgagt aaacagcgtc atcgagtctt ttgttttcga
      961 gatcgtaata attaatatca gagcctaaaa aacggccttc cttgccgcta tttccggatc
     1021 cgctgataag acgggatttt gcaagcgcgg ggaggtatag ccgctgctcg tctctgttat
     1081 ttgtgttaac cgataacagg cgtacaccgg cgatgtctgc cggatcagtg acttcgatat
     1141 agtttgcttc aaatgaatca cgatcgatgt tttgctggtt aaacgagcgg ctgagttttt
     1201 taccttttcc gtcaataagc atgagagata ttctggctgt atagcaggga gctgatttaa
     1261 ggttaaaatg atttttcatt attgcgaagg ctttgtcatc acaaaacgag ttttgaacgg
     1321 agcataaaat tactagaaaa aaaagtgata taaatcggag catgatattc tcctctgaaa
     1381 aaagtttagc gatacagtaa gtttaggagg tgaacctttg ctgtaatcgt tgtataaaac
     1441 ttatcagaag atatatcttt tgactgttgt ttttaaacaa gatgctttat ggcgcatgca
     1501 agatgcatta ttggaaggta aaaagcagaa taggggtgct ctgcccttgt tgctagcaga
     1561 gcttctgttg tttgtgtttt agaagcgagt taagccgtac agttttttta tacatgtaca
     1621 tgccaaatcc gattccaaac agttcggcaa agaaaataat aaacagatgt gttaatagtg
     1681 aattgatgcc atttattgac gctccaatat acgagccggc agcaataatt ataaattctg
     1741 ccacaaaggc cacaagtgga gttttgcgaa tggttctaaa tagccaggca aaggccagag
     1801 ttgcaaagaa tccaataatc agagctgtta aaacgctacg cactattccg ggtagcaggg
     1861 gatctttcag ataaagagtt acaggcgtgc caactgcaat accgataaac gcggaaacag
     1921 gtagtataac cagaaaatcc ttgatgatca ttatgattgc ttttttgctg ttgcttttct
     1981 tatacaccta ggtactcctt gaaatcagcg gaatatttgc gtgaaacctc tatttcgtcg
     2041 gttccgtttt tcatacgtaa caagagcctt cctccaaacc aaggagaaat gtccttgatt
     2101 tcgcgaacgt tgactatcgt ggatttgtta acacgaaaga atcccttgga cgacagcatg
     2161 tattccaatt cgtaaatttt tttctttatc tgcaagatct gacctttttt aatataacat
     2221 tcgatacggt tccgatcagc tgtaataaac tctatttcat caataggtac aagcataatg
     2281 cgctcgtcgt cgtagcagga tatcgtgtca acttcattgc tcgccattcg tccggtgtaa
     2341 agaatcattt ctattaactg gggcagcgaa tttgctttgt aaacaataca tatctctttg
     2401 tcgttaggga tggtgaaacc ctcttcgaca agcgcaatgg aggaggtgct gctttcgaca
     2461 ataccggctt ttagcagttc ttccttgttg ttcgcgataa ttgcaaattt ggtcattaat
     2521 gggtcacctg cggtaaaaat accaattttt taggttttag tcatttcaag attgttgaag
     2581 caaggcagaa aaaagtaaat aagaattttt ttcactattt gatttggtat taaagaagct
     2641 aggcggcctt ttttggaatg aatgtcaaac cagtataaaa gtataaggga acggtcttct
     2701 ttccttgaca aaatgtttgc gtatatcatt ttggtaaagg ttatttggtt gagagttgat
     2761 gaaagaggcc tgctaattat tatctacgct cttaaagttg aaatcagtga gaatttcggg
     2821 ggtaaaaagg ggcattatat ttaaaatcaa ctctatatta aaatgtttgc gtataacaaa
     2881 acaatataag tcagttgtaa aaggagattg tttatgaaaa agttgttatc cgttctgttt
     2941 ttggcagttg tcttgatcgc ttgcagttca aaagacgatg aggtaaagtt ttattatggc
     3001 gctgaggtct tttcgcataa accggttctg tttggcaagt ttgaagttag agccaagctg
     3061 attaatgaat cgggagtggt ttccagcttt tttctatatg ataatgcctc ttggcaagga
     3121 agaccatttc cttggagaga gatagatatt gaatctatag gtaaagaaaa aggtatgctt
     3181 cagaccaata ttattaccgg attcgcggat aaaagggtaa ccagtgaggg tgaaaccgta
     3241 attgaaaatg tgggcactga atacaagacc tatactatcg agtggacacc cgattatgtt
     3301 gcctggtttg tggacggaaa agagattcga cgcgatacag ccgaaagtag tcaacaggtt
     3361 gtggatctga gggatactcc acagacatac cggatgaatg tctggatagc gatatgggat
     3421 gagtgggttg gaaagtttaa ggagagctcg tttcctatgt atcaatcaat aaattggatt
     3481 aagtattatg aatacactcc tgaaaagagt gaaaaattta ctcataaatg gacagatgat
     3541 tttgatactt ttgatgagag ccgatggggt aagggtgact ggtcatttga gggaaatctt
     3601 gtcgtatttg taaagggcaa tgccgaaata aaagacggtc atcttgtttt ggggctaaca
     3661 aaggacgagt tcggttattc gggagaattt ctcaaggatg agtagtgtct ttgatactat
     3721 ctgatttttt tatagcaggt tagttgatgt ctggaatttt catgagtatt ttgatatgca
     3781 gttattcaaa gagaagctta aattatcaaa atttctccag taagtacagt ttaagtgtac
     3841 ttactggaga atgccaagtg taatagttta taccccaatt tttccttttt tataaataat
     3901 catatcacaa tgccatagaa tttaggtttt aaaatgattt tcgttagaga agtggattgt
     3961 tagccacaag gaagacggat ttgaacagtt ccctggattg ttagccacaa ggaagacgga
     4021 tttgaacatt ttcgtggatt gttagccaca cggaagaggg ttttgaacag ctttttggat
     4081 tgttagccag gcaaaaagtc aatttacggc aaaaaacacc accgtccctg tttatagtaa
     4141 agttacaatg tgattcccgt atgaggatta tggcgatgga ataaaggcat ggtcttttat
     4201 tattgaaata aaaaaatctt tagaccataa ctctacagaa tcgggatttt tgataaaggg
     4261 taagacatcc agtttcgcct gattaaaatc tatggaacaa attctttcca taagcttatt
     4321 tacaagagta gtattgttaa atgtgtcatc aatttgcaaa tgtcctgttt ggatcattct
     4381 cttgttcaaa tggttcaggt tgactttggc tcctttcgaa atgtaccaga ctaggtcgta
     4441 cagatcacgc ccctttattc tgttttgcca gttacggcaa agtaccgcgt gtaatttgcc
     4501 ggaaaaaagc gatggtaatg tatacaattg taccgtgaaa ggaattggaa taagatgata
     4561 tttcatttct gttgttgcac cttcgggagg atcaatatca atttcaaatt ttaccttaat
     4621 tttctcatca ggatgaactc cgctaacggg tggagtgaca gagttgattt ttaataaatg
     4681 gattagggtt cctcctttta taaacgcaga tttgatattg gtattcttca gtttggtctt
     4741 tgtttctata tcaagctcaa atccaaagga ttcaagttct gcctttacaa attttagaaa
     4801 cgaagaaaaa tcaaaatttt catttttttt tgttaaggtg aaatcaaggt cttctgaaaa
     4861 tcgctcaaga ccatgaaaaa tacgaagggc agtttcacca taaaatgcag ctttgctgta
     4921 aaagccgcta cggtaaagac ctagcagagt aatttcttga attatttctt tcaaagcatg
     4981 tatataatca aaatttgatt tgcagttata tttttctaac atagattcta cagcactgtg
     5041 cattatctct ccttttcttg caaatatgta tcgaatagat acatattttt attgttgtaa
     5101 agaggtgcta aaaaggatat tttagaagat tcgattttat taataaaatc ttcttcaaga
     5161 cgcatatctt caaataaaaa ttgctctatt tgatgtgttg tattgaagtt tttacaacgg
     5221 taaagaagat cgcataatgc tttttccgga gtagaaatca taaaataatc gttttctaat
     5281 ttctcaatga ttattccgta gggataaacg gcaggcggta tacagtaata atagaaatat
     5341 ccgacatcag ttttatatgt ctttcttttg tttttattat aaactgcgga agttacagaa
     5401 acagtttttt ccggaataag tccgtaatat gataaagcat attcaaaaga aatatatgat
     5461 ggtccgtaca gcaaattggc aataacctgg acagaagaaa acccttcaga tttatctata
     5521 taaaggcctc ttctgatctt taaaatatcg ttcttttgtg ccatccggct aattttggtc
     5581 tttggagaag agtaattttc aagttttttt aaaaggtatg tcgaatcaat tatcataaat
     5641 atctccagta agtacagttt aagtgtactt actggagaat gtcaagcgta atagtttata
     5701 ccccaatttt tcctttttta taaataatca tatcacaatg ccatagaatt taggttttaa
     5761 aatggttttc gttagagaag tggattgtta gccaccctga gggcgatttt gagtaatttc
     5821 ctggattgtt agccac
//
LOCUS       DAOTGZ010000175         5823 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_3064
            NODE_16297_length_5823_cov_3.960298, whole genome shotgun sequence.
ACCESSION   DAOTGZ010000175 DAOTGZ010000000
VERSION     DAOTGZ010000175.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868088
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 5823)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5823)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:22:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,785
            CDSs (total)                      :: 2,751
            Genes (coding)                    :: 2,732
            CDSs (with protein)               :: 2,732
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 12 of 19
            Pseudo Genes (internal stop)      :: 5 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5823
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16297_length_5823_cov_3.960298"
                     /isolate="AMR_MDS_3064"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(99..668)
                     /locus_tag="P5123_10545"
     CDS             complement(99..668)
                     /locus_tag="P5123_10545"
                     /inference="COORDINATES: protein motif:HMM:NF014620.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HRX16742.1"
                     /translation="MIHISKRRCVGCGICNSECPVGALVVDSDKGYAVVDNSKCLSCG
                     VCIARCPQRAIADITKSIVFAIGTDDGTFIKQKDHVGSSLSFMIYRYSEGKMVLIDKR
                     NNVKYDEDESETHGDPGKAAAVSDVLSGADVIVGSIIGPNVKRLRKRFLPIVVRTDTI
                     ENSLKIITENMPDISEELSKENMNALVLS"
     gene            complement(765..941)
                     /locus_tag="P5123_10550"
     CDS             complement(765..941)
                     /locus_tag="P5123_10550"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRX16743.1"
                     /translation="MRHQVEGELYNQLETFGGEQYRHIGFRDKKKSFGDMLEFLVPET
                     GMTKKVRITIEILE"
     gene            complement(1351..1908)
                     /locus_tag="P5123_10555"
     CDS             complement(1351..1908)
                     /locus_tag="P5123_10555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009779991.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00730 family Rossman fold protein"
                     /protein_id="HRX16744.1"
                     /translation="MNESCIKSLCVFCGSSVGSDPLYRQDAVLLGQMCARNSITLIYG
                     GGNSGVMGQLSKAVVANGGKAVGIIPELLNRNSKHDKEVETIVVDNMHGRKALMYQQS
                     DAFVALPGGIGTIEELFEVYTWKQIGYHEKPVALLNSGGYFDHLIDFLDNMVRKKFLN
                     KEHLADLIVAYNVDELFARLSAFFV"
     gene            complement(1908..2729)
                     /locus_tag="P5123_10560"
     CDS             complement(1908..2729)
                     /locus_tag="P5123_10560"
                     /inference="COORDINATES: protein motif:HMM:NF012334.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HRX16745.1"
                     /translation="MGSNTIKEKVVLITGAGGGLGRDIAKALAEMGGRIIVADINKKA
                     AMQCAEILNKAGLVAEAVVLDVSDRRSVIKCANTIIKKYGRVDILVNNAGIVVGKSFL
                     DSKDEENISTMEINAISNFWTVKAFLPGMIERGTGQIVTISSAAGLIGVPGLADYCAS
                     KFAVYGFHEAVRKEVKKKGKKDIRFTIICPYYINTGMFEGVKKSPLSFLLPLLDQKRT
                     VKKIVKAIVKRKRRLYMPPIVYVLPLLRVFPELVFDFIATLFGVHNSMDTFKGKK"
     gene            complement(2731..4134)
                     /locus_tag="P5123_10565"
     CDS             complement(2731..4134)
                     /locus_tag="P5123_10565"
                     /EC_number="3.1.3.16"
                     /inference="COORDINATES: protein motif:HMM:NF018884.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004722 - protein serine/threonine
                     phosphatase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PP2C family protein-serine/threonine
                     phosphatase"
                     /protein_id="HRX16746.1"
                     /translation="MDSFLTAYLNQNNSKMLRYMAYLFVLLGALFSVQHYFHTWNVLS
                     VVHYRWYMFLYYFLIVVSLFYILMSFTVLNSSKSPLFDRYINLFYVSMYLAVVIGLNI
                     LDLHYSLELVPLMMGLIFLVVLYRESPIVQILIIVLPLASVLTAIHLIVPDQKNVWML
                     TFYLSVFGFVAFFASHMLYRSFIERFIIMSELNDRNCQLDTLNTRLRSYKVSMERDLR
                     IAERIQQEYLQNRPGIPNGWDFDVRFVPKNGVSGDFYDIYCSGSELYGVSLFDVTGHG
                     VASSLVTMLAKRIFYRLFAAYRDTPLSQLAALFNLELLEEIGNSHIYIAGVVLKYDGK
                     GFEYINAGHTDILIKKADGSVRPVLDSHDERNPFFCLSEFTEGYGVKYFELQPGDTII
                     MATDGIYELQPAKGASVISYKGIENFIADLPAKKSAGYYSEKIVSYVSETSGRSDFDD
                     DMTLVVLCRKDKLVIGE"
     gene            4225..4905
                     /locus_tag="P5123_10570"
     CDS             4225..4905
                     /locus_tag="P5123_10570"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRX16747.1"
                     /translation="MNFLSHFYIQSIQNNNEYSVGVSLPDILSLYQNPRPVSSKIITE
                     IATKQNISPQESRLVCGMNAHYRADKIFHVSPWFANSMQSVCSLYTDIFNRHLPQSLS
                     HILVEILLDKYLIETNRDIISNFYDQYEKFDIKTAHCLFERCAFYDKSLFEKSLTRFK
                     TLRYIENYRTHSGIALTLKQISERLDIKTSVNFDLLSDFISSSYRMIEGECFIFFDKT
                     QKKMFKKS"
     gene            complement(4908..>5823)
                     /locus_tag="P5123_10575"
     CDS             complement(4908..>5823)
                     /locus_tag="P5123_10575"
                     /inference="COORDINATES: protein motif:HMM:NF018884.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016791 - phosphatase activity [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="SpoIIE family protein phosphatase"
                     /protein_id="HRX16748.1"
                     /translation="INSLMVFPMIFQEEVMGLMAFCNKEDGTFFTKGEFNLGKALSNH
                     VAIIINNFKFYNQLVKRQRDERELEIAGSIQKNLLPRAIPEYDSVDIFTYSRPAKGVG
                     GDYYNIIQVDENRIDVVIADVAGKGVPASLVMVMISTVLKNIIKSNYGPKRIISFLNK
                     FLCKESTLERYATLSYLSIDVVRDTALYTNSGHSPLLMYKALKDKFEQVDTPGIPLGI
                     DAEQQYYQKQTGLDKGDIMMMFTDGITEAMNESNEMFGLERLRATVLEAKDESAEVIG
                     NTLLERIEMFAAGAEQHDDLTLIIVKKR"
BASE COUNT         1880 a         1288 c          926 g         1729 t
ORIGIN      
        1 cgtacaaatt cgttttgctt aaatcaaaca gactccgtga cttttataac aattatcttg
       61 actgctgaat cataataact tcagtcatta tctgttagtt aactaagcac aagggcattc
      121 atattttctt ttgataattc ctcagaaatg tcaggcatgt tttccgtaat aattttcaaa
      181 ctgttttcaa tagtatcagt acgcacaaca attggcaaaa accgttttcg aagacgctta
      241 acgttaggcc caataatgct accaacaatc acatccgccc ctgacagcac atcagataca
      301 gcagcggcct ttcccggatc accatgagtt tcagactcat cctcatcata cttaacatta
      361 ttccttttat cgattaaaac cattttacct tcagaataac gatatatcat aaaagataat
      421 gaagatccga catgatcctt ttgtttgata aatgttccat catctgttcc aatcgcgaat
      481 actatagact ttgtaatgtc ggctattgct ctctgtggac agcgcgcaat acaaacacca
      541 catgacaggc atttactgtt gtctacaacc gcatatccct tatcagaatc aaccactagt
      601 gcaccaaccg gacactccga attacatatt ccacaaccaa cacatcttct cttactaata
      661 tgtatcataa tcaccgctcc tattctgtgg cagtacagtt acacagaccg gatattacta
      721 tgcattcaat agttataccg tatccggata atcacatatg cacttcattc gagtatttcg
      781 atagtaatgc gtactttttt tgtcataccg gtttcgggca caagaaattc aagcatatct
      841 ccaaatgatt ttttcttatc acgaaaacca atatgcctat attgttcacc accaaaagtc
      901 tccagctgat tgtacagctc tccttcgacc tgatgcctca taactgctcc ccattatttt
      961 atatatccat atattgtgtt acttggatat tgtcaagtta tttccaacta acattgttaa
     1021 ggttggctat atttatctta tttagtgtta attttgatag ttatcgtgca gatatgtttt
     1081 cacggcaggc gaattttact aaaaaagaaa atccaataca aaaaggtttg aattagggta
     1141 aaagtaattt atgaccagca agcctcctta gtccggttaa gaaggcagcc aatcataatt
     1201 atatattttt tattgttttt cacaaaagtg attattacaa ggagattaca aaggagtaca
     1261 aacaagccgg tattttaact aaaccttact gcctggtttt aagaataact tttacattga
     1321 tattgtgtaa acaatcaaca ctgatatttt ttacacaaaa aaagcggata accgggcaaa
     1381 gagctcatcc acgttatacg cgacaataag atccgcaaga tgttctttgt tcaaaaactt
     1441 tttcctgacc atattatcaa gaaaatctat taaatgatca aagtatccac cgctgttaag
     1501 cagcgcaacc ggcttttcgt gataacctat ttgcttccat gtgtatactt caaataactc
     1561 ttcaatagta ccaataccgc ctggtaaagc aacaaaggca tctgactgtt ggtacattaa
     1621 tgcttttcgc ccatgcatat tatcaacaac tattgtttcg acctctttgt catgtttgct
     1681 attgcggttc agcagctcag gaattattcc tactgcttta ccgccgtttg cgacaactgc
     1741 ttttgagagc tgccccataa cgcctgaatt gccgccacca taaataagtg ttatcgagtt
     1801 acgcgcgcac atttgaccaa gtaaaacagc atcctgtcgg taaagaggat cgctaccgac
     1861 acttgatcca caaaaaacac ataacgactt tatacagctc tcattcatta tttttttcct
     1921 ttaaatgtat ccattgaatt atgtacaccg aaaagagtag ctataaagtc aaaaaccagt
     1981 tccggaaaaa ccctaagtaa tggcagcaca tagactatag gaggcatata aagtcgcctc
     2041 ttccttttta ctattgcttt aactattttt ttcactgttc ttttctgatc taacagaggt
     2101 aaaagaaaac tcaacggact ctttttaacc ccttcaaaca tacccgtatt tatataataa
     2161 gggcaaatta tagtaaaacg gatatccttt ttaccctttt tcttcacctc tttgcgtaca
     2221 gcttcatgaa aaccatacac ggcaaacttt gacgcacaat aatcggcaag gcctggcaca
     2281 ccaatcagtc ccgcagccga agagattgtc acaatttgtc ctgtaccgcg ttcaatcatt
     2341 ccaggcaaaa aggctttaac ggtccagaaa ttcgaaatag cattaatctc catcgtgctg
     2401 atattctcct cgtcttttga atcgagaaaa cttttaccaa caacaatacc ggcattattg
     2461 accagtatat cgacccttcc atattttttg attattgtat tcgcacactt tattacagag
     2521 cgcctgtcag agacatcaag cactacagcc tcggcgacaa ggccagcctt gtttagaatt
     2581 tcagcacact gcatagctgc ttttttatta atatcggcaa caataattct accacccatc
     2641 tcggctaagg ctttcgctat atcacgtcca agcccgccac ctgcccctgt tatcaaaact
     2701 accttctctt taattgtgtt agaacccatc ttattctcca ataactagtt tgtctttgcg
     2761 acacaaaacg accagagtca tatcgtcatc aaaatcacta cgaccgctcg tttcacttac
     2821 gtacgaaact attttttcag aataatatcc agcactcttt ttcgcgggca agtcagcaat
     2881 aaaattttca attcctttat agctaattac gcttgcacct ttggctggtt gaagctcgta
     2941 aattccgtct gtagccataa tgattgtatc accaggttga agttcaaagt atttaactcc
     3001 atagccttct gtaaattcac tcaaacaaaa aaacgggttt cgttcatcat gtgaatcgag
     3061 taccggtctc acagatccat cggccttttt tattagtatg tccgtatggc cggcgtttat
     3121 atattcaaat ccttttccgt cgtatttcag aactacacct gctatataaa tatgcgaatt
     3181 tccaatctct tcaagaagtt caaggttaaa tagagctgca agttgcgata atggggtatc
     3241 acggtaagca gcaaagagtc gataaaaaat tctttttgcg agcattgtaa caagcgatga
     3301 agccactcca tgacctgtca catcaaaaag agacactcca taaagctctg agccagagca
     3361 atagatatca taaaaatcac cactaacccc gttttttggt acaaatcgga catcaaaatc
     3421 ccaaccattg ggtattccag ggcggttttg taaatactct tgctgtatac gttcggcaat
     3481 tcgcagatct ctctccatag acaccttgta cgaccgcagc cttgtattaa gagtatctag
     3541 ctgacagtta cgatcattaa gctcactcat tattataaaa cgctcaataa acgaacggta
     3601 aagcatatgt gaggcaaaaa aagcgacaaa accgaatact gatagataaa aggtcaacat
     3661 ccagacgttt ttttgatcag gaacaataag atgaatagcc gtcaacacgg atgcaagagg
     3721 taaaactata ataagaatct gaacaatagg actctctcta tacaatacga caagaaagat
     3781 aagccccatc ataagaggaa caagttccag cgaatagtga agatcaagga tattgagccc
     3841 tataactacc gcaagataca tcgatacata gaacagattt atataacgat caaacaaagg
     3901 tgatttcgaa ctattcaata cagtaaaaga cattaaaata tagaacagtg acaccacgat
     3961 aagaaaatag tataaaaaca tgtaccaacg ataatgcacc acagagagga cattccaggt
     4021 atggaaatag tgctgaacag aaaaaagagc tccaagcaat acaaaaagat aggccatata
     4081 gcgaagcatt ttgctattgt tctgattcag gtacgcggtt aaaaaagagt ccaccaaatt
     4141 caccgttaaa caaaaagata tagacacatt tacgctatca aagcatcgta taatatcaag
     4201 tctcttttgg aaactgaaaa aaatatgaat tttctatctc atttttatat tcaatcaata
     4261 cagaataaca acgaatactc ggtaggtgta agccttcccg atattctatc actgtatcaa
     4321 aatccaagac ctgttagcag caaaataatc actgaaatag caacaaagca gaatattagc
     4381 ccgcaagaga gccgtcttgt ctgcggaatg aacgcccact accgagctga caaaatattt
     4441 cacgtttcac cgtggtttgc aaattcaatg cagagtgtct gtagccttta tacagatatt
     4501 ttcaacagac accttcctca aagcctgtcc catatactcg tagagatact gcttgataaa
     4561 tatcttattg aaacgaatag ggatattatc agtaattttt atgatcagta cgaaaaattt
     4621 gatataaaaa cagcacattg cctttttgag cgatgcgcat tttatgacaa gtcactattc
     4681 gaaaaaagtc ttacacgttt caaaactctt cgatacatag agaattacag aacccactct
     4741 ggcattgcgc taactcttaa acaaatttct gaacgcctgg atattaaaac ttctgtaaac
     4801 tttgatctgt taagcgattt tatttcctcg tcttaccgca tgatcgaagg agaatgtttt
     4861 atattttttg ataagactca aaaaaaaatg ttcaaaaaat cttaatctta gcgcttcttg
     4921 acaattatca gcgtcaaatc atcatgctgc tccgctccag cagcaaacat ttcgatgcgc
     4981 tcaagaagtg tatttccaat tacctcggca ctctcatctt tggcctcaag tacagtcgca
     5041 cgtaagcgtt ctaacccaaa catctcatta ctctcattca tcgcctcggt tataccgtcg
     5101 gtaaacatca tcattatatc gcccttgtca aggcctgtct gtttctggta atactgctgc
     5161 tcggcatcaa tacctaaagg tattcccggt gtatccacct gttcaaattt gtctttaaga
     5221 gccttgtaca taagcaaagg gctgtgacca gagttcgtgt aaagagctgt atcccttaca
     5281 acatctatgg agagataaga aagcgtcgcg tatctttcca gagtactctc tttacaaaga
     5341 aacttgttta agaaactgat aattcgcttt ggtccataat ttgactttat aatattttta
     5401 agaaccgtac ttatcataac cataacaagc gatgccggaa cccctttacc tgcaacatca
     5461 gcaataacca catcaatgcg attttcatct acctgaataa tattataata gtcaccacca
     5521 actcctttgg cagggcggct ataagtaaaa atatcgactg aatcatattc tgggatcgca
     5581 cgtggtaata gatttttctg aatggaacct gcaatctcaa gttcgcgttc gtctcgctga
     5641 cgtttaacca gttggttata aaacttaaag ttatttataa taattgcaac atggtttgat
     5701 agagcttttc caaggttaaa ctcacctttg gtaaaaaagg tgccgtcctc tttattgcaa
     5761 aaagccatca agcccataac ctcttcctga aaaatcattg ggaaaaccat gagtgaattg
     5821 atc
//
LOCUS       DAOTGZ010000176         5791 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_3064
            NODE_16413_length_5791_cov_3.929045, whole genome shotgun sequence.
ACCESSION   DAOTGZ010000176 DAOTGZ010000000
VERSION     DAOTGZ010000176.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868088
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 5791)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5791)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:22:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,785
            CDSs (total)                      :: 2,751
            Genes (coding)                    :: 2,732
            CDSs (with protein)               :: 2,732
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 12 of 19
            Pseudo Genes (internal stop)      :: 5 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5791
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16413_length_5791_cov_3.929045"
                     /isolate="AMR_MDS_3064"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1411
                     /locus_tag="P5123_10580"
     CDS             <1..1411
                     /locus_tag="P5123_10580"
                     /EC_number="6.3.2.13"
                     /inference="COORDINATES: protein motif:HMM:NF001124.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016874 - ligase activity [Evidence IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanyl-D-glutamate--2,
                     6-diaminopimelate ligase"
                     /protein_id="HRX16749.1"
                     /translation="EKSLFSAVEGFKSDGHDYIMRAYKSGCRAFLISSQKSSIKSDYP
                     DACFITASESRVALSRVSALFYGEVSKKIPVIGVTGTNGKTTITYMIESVCKQAGMKP
                     GVIGTVNYRWLQTVEPAPNTTPESRDIHNIMARMMWDGVDVIIMEVSSHGLSLGRVDD
                     VHFAIALFTNLTQDHLDFHKDFEDYYQAKKKLFDLLQKSEAPVTNRYAVINCDDKYGE
                     RLVQELKGAAFELRDAGTGRGYYSLDPESVRAELSGISYHLNTPEASYDLDLHLTARF
                     NISNSLIAFGALDGLGIDHDKIVKGLCSIESVPGRFDRIASPLGFYVVVDYAHTDDAL
                     VKLLKAAREINPKRLITVFGCGGDRDKTKRPLMGHAAVSLSDIAFVTSDNPRTEDPQA
                     IVDDILLGIKADDGNYEVIVDRSEAIKRAVYYARKGDLLVIAGKGHEDYQIIGTQKSH
                     FDDKEVAAKFIEERVISEG"
     gene            1401..2771
                     /gene="murF"
                     /locus_tag="P5123_10585"
     CDS             1401..2771
                     /gene="murF"
                     /locus_tag="P5123_10585"
                     /EC_number="6.3.2.10"
                     /inference="COORDINATES: protein motif:HMM:TIGR01143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0047480 -
                     UDP-N-acetylmuramoyl-tripeptide-D-alanyl-D-alanine ligase
                     activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-tripeptide--D-alanyl-D-
                     alanine ligase"
                     /protein_id="HRX16750.1"
                     /translation="MKVDYCNSLCEIAKITGGELVGNGSVQISSICTDSRLCDNGSLF
                     IPIRGEKFDGHEFIDSLCEQKGIACYLTENNVQENKVPYVLCDNTLYALAKIAGSYRN
                     TFDLPVVGITGTNGKTTTKELIFTVLNGSYSVHRNIKNYNNEIGVPFTLLQFDNSHNA
                     AVIEMGMNHVGEIFRLSAIVRPDTAVITNIGAGHLEFLGTTQNVARAKSEIFSSMNAG
                     STVFINRDTIHYDIVSEAALEKKLVVKSYGLHKESDITPDSYLLKEDSIVLNYKGVQI
                     TAPLYGVHNVMNTLAAIAVGEHMGVTLDLCAERLKGFKNESMRGHITEGRCRVINDAY
                     NSNPLSLENALRSVGTIYSSSRTIAVLGDMKELGSKATDLHFECGAAVARYGFKRLYT
                     LGKLASQIAEGAKENGMDRATIFSFENKEKLVEALLKDIKKDDVVLVKGSRSMKMEEV
                     AEQLIR"
     gene            2779..3876
                     /gene="mraY"
                     /locus_tag="P5123_10590"
     CDS             2779..3876
                     /gene="mraY"
                     /locus_tag="P5123_10590"
                     /EC_number="2.7.8.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014801277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008963 -
                     phospho-N-acetylmuramoyl-pentapeptide-transferase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phospho-N-acetylmuramoyl-pentapeptide-
                     transferase"
                     /protein_id="HRX16751.1"
                     /translation="MFYHLLVPLQKYVSFLRLFQYITFRSLYAAVTALLFVLVFGNLI
                     IGWLRNLKFKEEIRALGPDSHKAKAGTPTMGGVIILGAILFSSMLWSNLLNLYNIVLM
                     SATLLLGAVGFADDYIKAILKRKDGMPGRMKLFFQILIALAAVLIIYFNPSNGEFTTK
                     IFIPFISKVDSSSLIDLGIFWIVFGAVTIVGSSNAVNLTDGLDGLAIGSVLIVSVTLG
                     IISYLTGLAPVAAYLNIPHIPDAGEISVFIAALAGASLGFLWFNSNPASVFMGDTGSL
                     ALGGIIGLIAVMIKKELLLFIVGGVFVIETVSVIIQVGYYKLRKKRIFKMAPIHHHFE
                     LSGWSEQKVVVRLWIIGIILALIGISTLKIL"
     gene            3885..5252
                     /gene="murD"
                     /locus_tag="P5123_10595"
     CDS             3885..5252
                     /gene="murD"
                     /locus_tag="P5123_10595"
                     /EC_number="6.3.2.9"
                     /inference="COORDINATES: protein motif:HMM:TIGR01087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008764 -
                     UDP-N-acetylmuramoylalanine-D-glutamate ligase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanine--D-glutamate
                     ligase"
                     /protein_id="HRX16752.1"
                     /translation="MKIDINKKGKVLVVGLGFRTGLTSANFLSQRGYKVTVSDNKSVA
                     ELTEIIKKLDKNVDVVVGKQSPEILDENFDFLVLSPGVPASIPLIVEANKRALPVIAE
                     VELAYYFMSGKWIAITGTDGKSTTTSLTNHILRTLGYDSREGGNIGVPLISLVEKSKE
                     DSVTVAELSSFQLETVFDFHPGIATLLNLAPDHLDRYDSMDHYLQAKLRIGMNMGSSD
                     YLVYNLDDEVIAYSAQQINAKGLSFSINDCNADCFYDGQTIFLKDENSKVKIDPSNLL
                     IRGIHNIKNFMASLLMIQSYIRLVGDEPDYSTILKAACDYPGLEHRLEYVATIDGVTY
                     INDSKATTVNSVKTAIAACGKDTVFIVGGRAKGENYGLLAEALRTGVRHSVLIGETAD
                     EFEKLFSHISHERAVSMEDAVKKARDFARSGDSVVLSPGCASFDMYKDFEERGRDFKR
                     CVAEL"
     gene            5337..>5791
                     /locus_tag="P5123_10600"
     CDS             5337..>5791
                     /locus_tag="P5123_10600"
                     /inference="COORDINATES: protein motif:HMM:NF013279.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsW/RodA/SpoVE family cell cycle protein"
                     /protein_id="HRX16753.1"
                     /translation="MDTRRKGVPDAVFFLTVFVLAGVGIAMSYSASAVFSLKTHGDSF
                     FILKKQLLWFVVGFVCLVFFQEIDYRHYKKFSKVFLLVSFVLLVMLLVPGVAHVVKGS
                     ARWIRVGGIGFQPSEFVKIFMVIYMAKVFSQDVSESANPLMQLLIPMML"
BASE COUNT         1616 a          954 c         1378 g         1843 t
ORIGIN      
        1 cgaaaaatca ctgttctcgg ctgttgaagg tttcaagagt gatggacacg attatattat
       61 gcgtgcgtac aagagtggct gtcgggcgtt tttaatatca tcacaaaaat cttccattaa
      121 atcagattat ccggatgctt gcttcattac tgcatcagag agccgagtcg cgttatcgcg
      181 ggtttcggct cttttttatg gcgaggttag taaaaaaata ccggttatag gagttaccgg
      241 tacaaacggt aaaacgacta taacatatat gattgagtct gtttgtaaac aagcgggtat
      301 gaaaccggga gttattggaa ctgttaatta ccggtggcta caaactgttg aaccagctcc
      361 taataccact ccggagtcgc gggatattca taatataatg gctcgaatga tgtgggacgg
      421 ggtggatgta ataataatgg aggtgtcatc gcacggctta tcgcttgggc gggtagatga
      481 cgtgcatttc gcgatcgctc tatttacaaa tctcacacag gatcatctag attttcataa
      541 agattttgaa gattattatc aggcgaagaa aaagcttttc gatttgcttc agaaatcgga
      601 ggcgccagtc acaaacaggt atgcggttat taactgtgat gacaagtatg gcgagcgact
      661 ggttcaggag cttaaggggg cagcgtttga attgagagat gcaggtaccg gtcgtggata
      721 ttacagcctt gatcccgaat cggtcagggc agaattgtcg ggtatcagtt atcacttgaa
      781 tactcccgag gcaagttacg atcttgacct tcatttaaca gcacgattta atattagcaa
      841 ttcactaata gctttcggcg ctcttgatgg gcttggcatt gatcatgata aaatagttaa
      901 aggtctatgt tctatcgaat ctgttccagg gcgttttgac agaatagctt ctccgttagg
      961 cttttatgta gttgttgatt atgcgcatac cgatgacgct ctagtaaaac ttttaaaagc
     1021 cgcacgagaa ataaatccca agcggttgat tacagtattt ggttgcggtg gtgaccgtga
     1081 caaaaccaaa cggcctttga tggggcatgc cgctgtttca ctttcggata tagcctttgt
     1141 tacatcggat aacccgcgta cggaagatcc gcaggccata gtcgatgata ttcttttggg
     1201 tattaaagct gatgatggta attatgaggt aatagtggac agaagtgaag ccataaagcg
     1261 agcggtttat tacgcgcgca agggcgatct tctggtgatt gccggtaaag gtcatgagga
     1321 ctaccagatt attggtacgc aaaagagcca ttttgatgat aaagaggtcg ctgccaagtt
     1381 tattgaggag cgtgttatta gtgaaggttg attactgtaa cagtttatgc gaaatagcca
     1441 aaattaccgg tggcgaattg gtcggtaatg gatcggtaca aatcagttcc atttgtactg
     1501 attcgcgatt atgcgataat ggttcgctct ttatccctat tcggggtgaa aagtttgatg
     1561 ggcatgagtt tatagattct ctttgcgagc aaaaagggat tgcttgttac ctaactgaaa
     1621 acaatgtgca agaaaataaa gtaccctacg tgctttgcga taataccttg tatgcgcttg
     1681 caaaaattgc aggcagttat cggaacactt ttgacctacc agttgtgggt ataacaggaa
     1741 caaacggtaa gacaacaacc aaggaactta tctttactgt cttaaacgga tcgtatagtg
     1801 ttcatagaaa tataaaaaat tataataatg aaataggtgt accgtttacc cttttgcagt
     1861 ttgataacag tcacaatgct gccgtaattg aaatgggtat gaaccatgtt ggagaaatat
     1921 tcaggttaag cgcgattgtt cgtccggata cagctgttat tacaaatatt ggagcaggac
     1981 atttggagtt tttaggtaca acacaaaatg ttgcgcgtgc caagtcagag attttcagtt
     2041 caatgaatgc cggcagtaca gtttttatta atcgcgacac aattcattat gatattgtga
     2101 gtgaagctgc acttgaaaaa aaattggtag tcaaaagcta tggactacat aaagagtcgg
     2161 atattactcc tgattcttat cttcttaaag aagattcaat tgttttgaat tataaaggag
     2221 tgcaaatcac agctccacta tacggagtac ataatgtaat gaataccttg gcagctattg
     2281 ctgttggtga gcatatggga gttactcttg acctttgtgc cgagcgttta aaagggttca
     2341 agaacgaatc gatgcgcgga catattacgg aaggtaggtg tcgagttatt aatgacgcgt
     2401 ataactctaa tccactttca cttgaaaacg cattgaggtc ggtaggaact atttattctt
     2461 catcacgaac aattgctgtt cttggtgata tgaaagagtt gggctctaag gcgaccgatc
     2521 ttcattttga atgtggtgct gcagttgcaa gatacggttt taaaaggcta tacactttag
     2581 gtaagcttgc atcacaaatt gctgaaggag caaaagaaaa tgggatggat cgcgcaacta
     2641 tttttagctt tgagaacaag gaaaagctgg tagaggcttt gttaaaagat atcaagaaag
     2701 atgatgtggt gctcgtgaag ggctccaggt ctatgaagat ggaggaggta gcggagcagc
     2761 ttatccgctg aaacgattat gttttatcat ttattggtgc cgttgcagaa atacgtctct
     2821 tttttgagac tttttcaata tatcacattc agatcgcttt acgcggctgt tacagcattg
     2881 ctttttgtac ttgtttttgg caacctgata attgggtggc ttcgcaacct caaattcaaa
     2941 gaagagattc gcgctctggg tcctgattca cataaggcca aggcggggac tcctactatg
     3001 gggggggtta taatacttgg agcgattctg ttttcatcca tgttgtggtc aaacttgctc
     3061 aatctttaca atatagtttt aatgtcggct acgttacttc ttggagctgt aggttttgcg
     3121 gatgactata ttaaggcaat tttaaagcgg aaagatggta tgccggggcg aatgaagctt
     3181 ttttttcaga tacttatcgc tttagcagct gttctgataa tttattttaa cccttcaaac
     3241 ggagagttta caacaaagat atttataccc tttatttcaa aagttgacag cagttcattg
     3301 attgatcttg gaatattctg gatagtattt ggcgctgtaa caatagtagg ctcttctaac
     3361 gcggttaatc ttactgatgg acttgatgga ttggctattg gttctgtact tattgtcagc
     3421 gttactcttg gaataatatc gtacctaact gggctagctc cggtagcggc atatttgaat
     3481 ataccacata tacctgatgc cggtgagata tctgtcttta tcgctgcgct tgctggggcg
     3541 tcgcttggat ttctctggtt taactctaat ccggcttctg tttttatggg cgatactggc
     3601 tcacttgctc ttggtggtat aatcggattg atagctgtaa tgattaaaaa ggaacttctg
     3661 ctttttattg ttggcggcgt attcgtgata gaaacagttt cagtgattat acaggtggga
     3721 tattataaac taagaaaaaa acgtatcttc aagatggctc ccattcatca tcattttgaa
     3781 ttatcaggat ggagtgaaca gaaggtagta gtgcgattat ggattatcgg gattatcctt
     3841 gcgcttattg ggatctcaac attaaaaatt ctataggtat aatagtgaag attgatatta
     3901 ataaaaaagg caaagtgctt gttgtaggtc ttgggtttcg aacaggtctt actagtgcca
     3961 actttctttc acaacgcggt tataaagtta ccgtatcgga taacaagagt gtcgcggaac
     4021 ttacagagat tataaagaaa cttgataaaa atgtggatgt tgttgttggt aaacagtctc
     4081 cggaaattct ggatgaaaat tttgattttt tggttttaag ccctggcgtt ccggcatcga
     4141 ttcctttaat tgttgaagcc aacaagagag cattgccagt aatcgcggag gttgaactag
     4201 cttactactt tatgagtgga aaatggatag ccattaccgg taccgacggt aaatcgacaa
     4261 cgacctcttt aacaaatcat attctaagaa ctcttggcta tgattcaagg gaggggggta
     4321 atattggtgt tcctctgatt tcacttgttg agaagtctaa agaagatagt gttactgtag
     4381 cggagctctc gtcttttcag cttgagactg tatttgattt tcacccaggt attgcgacct
     4441 tgcttaatct tgcgcctgat catcttgaca gatatgactc tatggatcat tatttacaag
     4501 ctaaactaag aattggtatg aatatgggtt cttcggatta tctggtttat aatcttgatg
     4561 atgaggtaat tgcatatagt gctcaacaga taaacgcgaa aggtttatct ttttctataa
     4621 atgactgtaa tgctgactgt ttttacgacg ggcagacaat atttttaaaa gatgaaaaca
     4681 gtaaagtaaa aattgatcct tcaaatcttt tgataagagg tattcacaat atcaagaatt
     4741 tcatggcatc gttgcttatg attcaatctt atattaggct ggtcggtgat gagcctgatt
     4801 atagcacaat tttgaaagct gcgtgtgact accccggttt agagcatcgt cttgagtatg
     4861 ttgcgacaat tgacggcgtt acttatataa atgattcaaa ggcaaccact gttaattcgg
     4921 ttaaaaccgc tattgctgca tgtggcaaag acacagtatt tattgtgggt ggccgtgcaa
     4981 aaggtgaaaa ttacggtctt ttagcagagg cactgcgcac gggtgtgaga cattcagttt
     5041 taattgggga aactgcagac gagtttgaaa aactattttc tcatataagt catgaacgtg
     5101 ctgtctcaat ggaggatgct gttaaaaaag cgagagattt cgcgcgaagt ggtgattccg
     5161 ttgtattgtc accgggatgt gcctcttttg atatgtacaa ggattttgaa gaacgtggaa
     5221 gagatttcaa aagatgcgta gctgaattgt aataatagaa ttgttgtaga tgatatttgt
     5281 gtataaacct tttaactgta acaataggga ggaataatct ggatctcagg actttagtgg
     5341 atacacggcg aaaaggtgtt cctgacgcgg tttttttcct gacagttttt gtgctggctg
     5401 gggtagggat tgctatgagt tattcggcgt ccgcggtttt ttcactaaaa actcatgggg
     5461 attctttttt tattctcaag aaacagttgc tctggtttgt tgttggtttt gtatgtttgg
     5521 ttttctttca ggagattgac tatagacatt acaaaaagtt tagcaaggtt tttctgcttg
     5581 tttcgttcgt tttgcttgtt atgttgttag tgcctggagt tgcgcatgtg gtaaaaggat
     5641 ccgcaagatg gataagggtt ggcggaatcg gttttcaacc ttcggaattt gttaagatct
     5701 ttatggtaat ttatatggcg aaggtttttt cgcaggatgt tagtgaatct gctaatccat
     5761 tgatgcagct gcttattcca atgatgcttt t
//
LOCUS       DAOTGZ010000177         5762 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_3064
            NODE_16527_length_5762_cov_4.719117, whole genome shotgun sequence.
ACCESSION   DAOTGZ010000177 DAOTGZ010000000
VERSION     DAOTGZ010000177.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868088
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 5762)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5762)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:22:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,785
            CDSs (total)                      :: 2,751
            Genes (coding)                    :: 2,732
            CDSs (with protein)               :: 2,732
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 12 of 19
            Pseudo Genes (internal stop)      :: 5 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5762
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16527_length_5762_cov_4.719117"
                     /isolate="AMR_MDS_3064"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1414
                     /locus_tag="P5123_10605"
     CDS             <1..1414
                     /locus_tag="P5123_10605"
                     /inference="COORDINATES: protein motif:HMM:NF013069.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HRX16754.1"
                     /translation="PDLFQIIVEAPDGTSLEETEEMVKVIEKMVIETVPAEVLQGLAS
                     EVGHHDTDYFGVSLGLHSEWALLTVNLIPSSKRKITCEELIEKIIPKAQLLKDKLNFV
                     KIETGIPAALPVGKALDIAVVSDDDNERIVYEKQIIDFLYTLEGVKNIQTNSLVGKDE
                     LVVLPDYKELARMGLTSADVAMAIRSAFEGEVVTTSRIDGEKVNYRVKLLDSRKFRED
                     MLLRIPVANAEGRMVRLEHVVTLAEQKADIMIIRENGKRSVNITADVDADIITSSKLN
                     KKVSDFLTDKPSPQTHFVFGGQAAESAESLSGFLFAGVFVFVSIYFILVILFDSYVLP
                     TLIMSIIPFAVCASLLTLVVHGRPLIMISMLGMLGLIGVVVNDTIVMITNLSANCVAK
                     KYTIKMCAKAASARLRPVVLTTLTTFAGLLPAAYGVGGDIPSVRPMVLVMAWGLLFCT
                     VVTLVFVPVLYSIVRPPAKR"
     gene            1517..3754
                     /locus_tag="P5123_10610"
     CDS             1517..3754
                     /locus_tag="P5123_10610"
                     /inference="COORDINATES: protein motif:HMM:NF012742.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GGDEF domain-containing protein"
                     /protein_id="HRX16755.1"
                     /translation="MALTASCHAYRTVFVWITSRGVGMLAKKIRIAVVMDIAFSVFQE
                     EPFIGVKRYLAETDIEAIFFGVGGLDINNPEDRMRMRFLDMITPEEFDGIILITSTLL
                     HCGGDQLLREKMALCRSSMPIVSIGPPIMGEDALLYNNETGMNQLMNHLIMDHGYREF
                     AYVSGPLSGAESQIRFGAFRDSLKEAGLEFNTNLVYEGNFLPPSGYDAVTEFFDNRKL
                     SPRVIVCANDFMALGVWNALIDRGINVPFDVAVTGYDDSQLSQALSHQFTTVRQSFNT
                     LGYLAAKRLHCLIRKEAVAPVLPLESELRIRSSCGCIEFERRVDKNRAESSVAGLDDI
                     KGKVLSCIKTNDPDCDPDEIYRSWSDIVHGTLRGKRPVYELEDMLRDSSNRLTNIGLD
                     IKNNQLFITLYSLLLEECNQSVFVDFWNENFFSMNLRVSVDNLHAGLMKNPSLYACDD
                     LMDNIAELCGANNFITVYFNDYHDLKSGSKVAYCKGGDFAKEYPGFGCWFPNRGAGSM
                     VANMISYNDECYGYMLLDALVEPANTFDYLRVRFSSISKDIDSLKNIMALNQELVHEI
                     EQRETVQKKLEAALTLVERLSVEDELTGLRNRRGFLALAEQQVKYLRRQDNPFYILYA
                     DLDKLKSINDTYGHHEGDLAIKCAADVLRSALRESDIIGRMGGDEFTVLVNKAKPPTF
                     DMIQQRVQKICDNKNRELNKPWALSMSIGRFYSDENCLLSLEKMIDFADEDLYKEKQK
                     KREMK"
     gene            complement(3758..>4431)
                     /locus_tag="P5123_10615"
     CDS             complement(3758..>4431)
                     /locus_tag="P5123_10615"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="GGDEF domain-containing protein"
                     /protein_id="HRX16756.1"
                     /translation="ASEECWEISNELVLTTQKAYEKKIISFKIISGVLSAITLGLITV
                     VWFIRYYVRKRLEYAVSHDTLTRILNRHSFNIYLDTELARYNRYNTNLSLVIFDIDKF
                     KNINDAYGHKAGDSVLHSVSRAVSKVIRSTDLFFRIGGEEFAIIAPGTSLKESVYLSE
                     KIREAVQDTRCIHKIKVTISVGTVQATRKETSETLFEKADQAMYKAKESGRNQVATNA
                     DTNNI"
     assembly_gap    4432..4531
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(4701..5372)
                     /locus_tag="P5123_10620"
     CDS             complement(4701..5372)
                     /locus_tag="P5123_10620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008506342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-lyxose/D-mannose family sugar isomerase"
                     /protein_id="HRX16757.1"
                     /translation="MKRSQINTIINDAAKFMKSNGFNLPPYAFWTIDDWRANKESCKE
                     ITSVMMGWDITDYGLGRFNECGLVLFTLRNGTPTDRTSKPYAEKIMISRQNQIAPMHY
                     HNNKIEDIINRSGATLAIELYNHTPDNELADTDVTVNSDGCLYNQKAGYIVKLSPGES
                     ITLPQGQYHKFWGYGGDVLVGEVSTINDDNTDNVFYEKVGRFPEIEEDEEPQYLLACD
                     YDKFI"
     gene            complement(5572..>5762)
                     /locus_tag="P5123_10625"
     CDS             complement(5572..>5762)
                     /locus_tag="P5123_10625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011770242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="putative beta-lysine N-acetyltransferase"
                     /protein_id="HRX16758.1"
                     /translation="VTMEKAMRERSMKTSFTIARSHSYGMNITFAKAGYKFCGTLINN
                     TNISGEIESMNVWYKHLA"
BASE COUNT         1546 a         1024 c         1258 g         1834 t
ORIGIN      
        1 tcctgacctg ttccaaataa ttgttgaggc accggacggc acttcccttg aagagaccga
       61 agagatggtt aaggtgatag aaaaaatggt gatagaaact gtacctgcgg aggtgcttca
      121 agggttggct tctgaggttg gacatcacga tacagattat tttggtgttt cattggggct
      181 tcactccgaa tgggctctat taaccgtcaa tctgatcccc tcttcaaagc gcaagataac
      241 ctgtgaagag cttatagaaa aaataatccc caaggcgcaa ttgcttaaag ataagctgaa
      301 ttttgttaaa attgaaacag gtatacccgc ggcgttacct gttggcaaag cgttggatat
      361 cgcggtcgta tcagatgacg acaatgaacg tatcgtttat gaaaagcaga taattgattt
      421 tctatatacg ctagaaggag ttaaaaatat acagacgaat tcactagttg gtaaggatga
      481 gttggtagtt ttgcctgact acaaagagct cgccaggatg gggctaactt ctgccgatgt
      541 tgctatggca attcgctcgg cttttgaggg agaagtagtt acaacgagcc gtatcgatgg
      601 tgaaaaagta aattatcgtg taaagcttct ggatagcagg aagtttcggg aggatatgct
      661 tttaaggata cctgtcgcaa atgcagaggg tcgcatggtt cgcttagaac atgttgtgac
      721 tcttgcagaa cagaaagcag acataatgat aatccgcgaa aacggcaagc gttcggtaaa
      781 tattactgcg gatgttgatg ctgatataat tacctcctca aagttaaata aaaaagtttc
      841 tgattttctt acggataaac cttcgccgca gactcatttt gtatttggag ggcaagccgc
      901 cgagtctgcg gagagtcttt ccggatttct ttttgcggga gtgtttgtgt ttgtttcaat
      961 ctactttata cttgtgatac tttttgactc ttatgttcta ccgacactaa tcatgtcaat
     1021 tattccattt gcagtttgtg cctctttgtt aacactggtt gttcacggca ggccgcttat
     1081 aatgatttcg atgctgggca tgctggggct tatcggtgta gtcgttaatg atacaattgt
     1141 aatgattaca aatctttcgg caaactgtgt tgcaaagaaa tacactatta aaatgtgcgc
     1201 aaaagctgca tccgcgcgtc ttcgtccggt agttttaaca acccttacaa cttttgccgg
     1261 gcttctgcct gcggcttacg gggtaggggg cgatatcccg tctgtgcgtc caatggtttt
     1321 ggttatggct tgggggcttc tgttttgtac agttgtaacg ctggtatttg ttccagtact
     1381 ctattctatt gtaaggccgc cggccaagag atgaccactg ttaatacaat gattttaaca
     1441 aagtagataa ttttaataaa aatttgctaa ttttcgacag atattgtata ttttttctga
     1501 catgattgat gttgtaatgg ctttgacagc tagttgtcat gcctatagaa ctgtttttgt
     1561 ctggataact tcaagaggag taggtatgct tgccaaaaag attcgcattg ctgtagtcat
     1621 ggacatagcg ttttccgtgt ttcaggaaga gccgtttata ggtgttaagc gttatcttgc
     1681 tgaaacagat atcgaagcca ttttcttcgg tgttggcggt cttgatataa ataatcctga
     1741 agatagaatg cgtatgcgtt ttcttgatat gattactccg gaagaatttg atggtattat
     1801 actgattact tcgactcttc ttcattgtgg tggagatcaa ctattaagag aaaaaatggc
     1861 attgtgtcgc tccagtatgc ccatagtctc tataggtccc cctataatgg gtgaagatgc
     1921 gcttctttat aacaatgaaa ctggcatgaa tcagttaatg aatcatctga taatggatca
     1981 tgggtaccgc gagtttgcct atgtctctgg tcctctttcc ggagcagaat ctcagattcg
     2041 gtttggagct ttcagagatt cactaaaaga ggctgggctt gaattcaata caaatcttgt
     2101 ttatgagggt aattttctac ctccatcggg atacgatgcc gtgacagaat tttttgataa
     2161 tcggaaatta tcgcccagag ttattgtttg tgctaacgat tttatggcat taggtgtctg
     2221 gaatgctctg attgaccgtg gcataaatgt accgtttgac gtagctgtga ccggatatga
     2281 tgatagtcag ctttcacagg cgctttctca tcaatttacc accgtacgtc agtcatttaa
     2341 tactcttgga taccttgccg caaaacgtct tcattgtctg attcgaaaag aggctgttgc
     2401 ccccgttttg ccactcgaat cggaactcag aatccgtagc tcttgtggtt gtatagaatt
     2461 tgaaaggcgt gttgataaaa acagggctga atcgtcagtt gctggactag atgacatcaa
     2521 aggtaaggtg ctctcctgca taaaaacaaa tgatcccgat tgtgatcccg atgaaattta
     2581 tcgctcgtgg tccgacatcg ttcatggtac attgcgtggc aaaaggcctg tatacgagtt
     2641 ggaagatatg cttcgtgact cgtcaaaccg tttaaccaac attggtttag atataaaaaa
     2701 taatcagctc tttattactt tatattcctt gttgctggaa gagtgtaatc agtcagtttt
     2761 tgttgatttt tggaacgaaa attttttttc gatgaatctt cgtgtgtctg tggataatct
     2821 gcatgcgggg ttaatgaaga atccgtcact ttatgcatgt gatgatttaa tggacaatat
     2881 tgccgagttg tgtggggcaa ataactttat tacagtatat tttaatgatt atcatgacct
     2941 taaatcaggt tctaaagttg cctattgtaa aggtggtgat tttgcaaaag aatatccggg
     3001 atttggttgc tggtttccta atagaggagc gggatcaatg gtcgcgaata tgatatcgta
     3061 taatgatgag tgctatggtt atatgcttct tgatgcgctg gttgaaccgg ccaatacttt
     3121 tgattatttg cgtgtaaggt tttcaagcat aagcaaagat atagattcgt tgaaaaacat
     3181 tatggcgtta aatcaggaac tggttcacga aattgagcag cgtgaaacgg ttcaaaaaaa
     3241 acttgaggct gctttaacgc ttgtcgaacg tctttcagta gaggatgagc taactggact
     3301 tagaaacaga aggggcttct tagccttggc agaacagcag gtcaagtatc ttcgtcgtca
     3361 ggataatccg ttttatattc tttatgccga ccttgacaaa ttgaaatcaa taaacgatac
     3421 ttatgggcat catgaaggtg atttagcaat caagtgtgct gccgatgtgc tacgctcggc
     3481 tttgcgagaa tctgatataa ttggacgtat gggcggtgat gaattcactg ttcttgtgaa
     3541 taaagcaaag ccgccaacat ttgatatgat acagcaacgt gttcaaaaaa tttgtgataa
     3601 taaaaataga gaactcaata agccctgggc tctatctatg agcataggac gtttttactc
     3661 tgatgaaaat tgcttattgt cgcttgaaaa gatgatcgat tttgcggacg aggatttgta
     3721 taaagagaaa cagaagaaaa gagagatgaa ataaatatta tatattattt gtgtcggcat
     3781 tggtcgccac ctgattccta ccagattctt ttgctttgta cattgcttga tccgcttttt
     3841 caaacagagt ttctgaagtc tcttttcttg tagcttgtac cgtgccaacg ctaattgtta
     3901 ccttgatctt atgtatacat cttgtgtctt gtacagcctc ccgtattttc tctgataggt
     3961 aaaccgattc tttcagggag gttccaggtg ctattattgc gaactcttcg cctcctattc
     4021 taaaaaaaag atcggtagat cgaatgacct tcgatactgc acgcgagaca ctgtgtaata
     4081 ccgagtcgcc tgccttgtga ccataggcgt cgttaatatt tttgaattta tcaatatcaa
     4141 aaattaccag ggataaattg gtgttgtatc ggttgtatct tgctaattca gtatcgaggt
     4201 aaatattgaa ggagtgccgg tttaaaatgc gtgttaatgt gtcatggctt acagcgtatt
     4261 caagcctttt tcttacatag tatctgataa accagactac cgtgattagc cctagtgtaa
     4321 tagcagacaa aaccccagat attattttaa aactgattat ttttttttcg taagcttttt
     4381 gcgttgttaa aaccagctcg tttgatattt cccaacattc ttcagaagca annnnnnnnn
     4441 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     4501 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nctccctcgt attcgcccag ctgtattgat
     4561 aatattgccc tcgtgttcaa atcgcaaaaa ttgaaaatag gcgtatccag ctaatattgc
     4621 tgaaagtata agaattagta aagatatata aaaagccgca tttgtttttt ttgatgagta
     4681 gcgcataata taccaacgta tcaaataaat ttatcataat cacaggcaag aagatattga
     4741 ggctcctcat cttcttctat ttcaggaaac cttcctactt tttcgtaaaa aacattatcg
     4801 gtattgtcgt cgttgatggt cgatacttca ccaactagaa catctcctcc gtaaccccaa
     4861 aacttgtgat actgcccctg tggaagagtt atgctctcgc ctggagaaag ttttacaata
     4921 taacccgcct tttggttgta caggcacccg tcgctgttta ctgtgacatc agtatctgca
     4981 agttcgttgt cgggagtgtg gttgtaaagc tcaattgcta gtgtggctcc gcttctgtta
     5041 ataatgtctt cgattttgtt attgtgataa tgcatcgggg cgatctggtt ttgtcggctg
     5101 atcatgattt tctcggcata gggtttggat gttcggtcag ttggtgtccc gtttcgaaga
     5161 gtaaaaagaa caagtccgca ttcgttaaaa cgtcccaatc cgtaatcggt tatatcccaa
     5221 cccatcatca cagaggtgat ctctttgcag gattctttgt ttgctcgcca atcgtcaata
     5281 gtccagaaag cgtagggtgg caggttaaaa ccgttagact tcataaattt ggcagcatcg
     5341 ttaataatgg tattaatttg tgaacgtttc atggcagcct cgattttttg taagtgtata
     5401 ggtttatata gtgtaaagag atgcctgcga agtaaatcaa tttttcattt tttctagatg
     5461 gactgaattt aaaaaacaga tatgctttag agttagaggg tagtgcttca tattgaaaaa
     5521 cagtgggatc ccgtagttgg cagaccctat cgcacactac gggatcatct tttacgcaag
     5581 atgcttgtac cagacattca tgctttcaat ttcaccggaa atgtttgtgt tgtttataag
     5641 agttccacaa aatttatagc ctgcttttgc aaaggttata ttcattccgt aagagtggct
     5701 ccgtgcgatt gtaaaagatg ttttcatcga acgttctcgc atggcctttt ccattgtgac
     5761 aa
//
LOCUS       DAOTGZ010000178         5762 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_3064
            NODE_16528_length_5762_cov_4.509024, whole genome shotgun sequence.
ACCESSION   DAOTGZ010000178 DAOTGZ010000000
VERSION     DAOTGZ010000178.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868088
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 5762)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5762)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:22:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,785
            CDSs (total)                      :: 2,751
            Genes (coding)                    :: 2,732
            CDSs (with protein)               :: 2,732
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 12 of 19
            Pseudo Genes (internal stop)      :: 5 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5762
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16528_length_5762_cov_4.509024"
                     /isolate="AMR_MDS_3064"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            425..646
                     /locus_tag="P5123_10630"
     CDS             425..646
                     /locus_tag="P5123_10630"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRX16759.1"
                     /translation="MNVVREIVKPINHVITINLPEGFTSNEEVEVIILPILKENSRKE
                     IRVEKFLNLAGKIEIDENAVNGLRENSLL"
     gene            643..876
                     /locus_tag="P5123_10635"
     CDS             643..876
                     /locus_tag="P5123_10635"
                     /inference="COORDINATES: protein motif:HMM:NF013970.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PIN domain-containing protein"
                     /protein_id="HRX16760.1"
                     /translation="MILVDTNIIIDFWKNPESKICDIFIQEDIATCGIILAELIHGAK
                     SEKESAIIQEALSAFNYLDIRKNDWVGIGSLIN"
     gene            complement(953..1573)
                     /locus_tag="P5123_10640"
     CDS             complement(953..1573)
                     /locus_tag="P5123_10640"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRX16761.1"
                     /translation="MKKIIVIMLLVPMILTAQAADKKEDSTKKSGSSTEKDFPTQMID
                     SDEFAIRHVSFNKKVDPAGKGELLQVDFQLENEVDVDLDLYIHIIVTHEELVWVYDSF
                     GEKKIVPKKIDTRYFSSLPDDRSLFEYEIDGEKTIVKYPKNIKAGVNTATGKPYHLSD
                     YMAFRYEFLCHYRRKYEFFNKATILIFDDSEKLLFRQSYSLDKYRR"
     gene            complement(1596..2993)
                     /locus_tag="P5123_10645"
     CDS             complement(1596..2993)
                     /locus_tag="P5123_10645"
                     /inference="COORDINATES: protein motif:HMM:NF012725.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HRX16762.1"
                     /translation="MKKSCVMFPLLLTIMLLRAHYLLAQTVEQYNSSGVEKSKNGNYY
                     EAEKDFNSGVKKSDEGAALVYHNLGWSYEQQGDLKNALINYEEAIKRNPRQIVSYERA
                     GYVSYKLGQHQKAVEIGEEGMRLDPTNEEIPTWLTSAYRERYSRNKYLLDDPNRTFKP
                     TKRNVFTAGVAICGPLGISTDDYSIEYLDNPGPFSADFSIEAYGEYNHKNRWGARLYV
                     GNPYFGTLMPDTIILTERVEGYIIGEAHYIGAGLMVSHYQGDHHFSKDMLLHDFKIGI
                     VYGYENDKNRLDMRWYPRLIPHETGYGSERYLDTALLEVDWVRAYRRSFDLSFGFKSS
                     EFYFFDNKADEPVSDYNGVYEVKAGVIFNNNASAFHRIHLHLIERIYMSDYNNRNPYS
                     SLNGQGMFGINTRKWLKGDPLSGISTLSHVISAKFEQRFSDNFVLYEKIMLEAVMPSR
                     AYHGAALQIGIEGGY"
     gene            complement(2990..4459)
                     /locus_tag="P5123_10650"
     CDS             complement(2990..4459)
                     /locus_tag="P5123_10650"
                     /inference="COORDINATES: protein motif:HMM:NF024824.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HRX16763.1"
                     /translation="MKKYKFKCTDVKIVKFTVIAVLFVVQMFCASPVLSAQTSDSLNK
                     RGVERAKDGLHERARDDFSRAVSIYNRESARLLHNKGVMFEKRGNIDDAIAAFEEAVR
                     RNPRQAVSFERLGYLNYKQGRYSKAVTYGEKALEIDPDNRQVKSWIHDAYRKKIDTIE
                     PTEEKEVAVNRDPIWQGKSPYQRIDPYRGGFYGTFDFSLRFFYQPDEDSVSGYQKSEG
                     ILTNFPYLMQAYYRPFDKIGFFGSLEHPYTGLGFADVVSQSERVECLYYMGRLVVGGG
                     LLLNHYDGNIFQDKKQTLHDYKLGGSLHYEGEDYIMDLLFYPRLLLLDWSFLSGTSYD
                     TAQFEFKYTYLFKPDFIYYTRIASYDYYFINHDDPLSDYYGFFDAAIGATVSNYSFFP
                     GVDLKLTAEVGLRQYIIKTVDDPYSFLNGQSFLGIDKNRDNGSLVSGQDQKTTIFQLR
                     FDERFSENFFIYQSVLLELAGYESDRHEYVIRFGAGYIR"
     gene            4782..5627
                     /gene="bioB"
                     /locus_tag="P5123_10655"
     CDS             4782..5627
                     /gene="bioB"
                     /locus_tag="P5123_10655"
                     /EC_number="2.8.1.6"
                     /inference="COORDINATES: protein motif:HMM:TIGR00433.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004076 - biotin synthase activity
                     [Evidence IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0009102 - biotin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biotin synthase BioB"
                     /protein_id="HRX16764.1"
                     /translation="MNNSHPIAIELCSIVNIHSGNCSENCLFCAQSRHNSCDIEKFDS
                     IDPNNLVNHAKMLQAHGVKYISLVASGRGPSKELLATVTALTEKILSNTTLQVCCSLG
                     LLTKSDANFLKTAGISRYHHNLETGESFFPQVCSTHSFDDRIRTIENAREAGLEICLG
                     GMINIGESMDDRINMVETAIKKGATSVPLNVLLPVEGTPLYGKILMDPDELIYTITTL
                     YSMFPDTIFRFAGGRSQYSEEITIKSLKAGIRGIMTGNYLTKAGIPVDKDIKLIGQNG
                     YSVAS"
BASE COUNT         1814 a         1205 c         1000 g         1743 t
ORIGIN      
        1 atcgacgata tgcgtttcgc tgtaggtgga ctgtttttta tgttatcgga tactattttg
       61 tctcaggata tgctactaaa aaggaggggg caagcttcac ttctagtttt gcttctctat
      121 tacaatgctc agcttttact tactcttgca gtcgctggca ctctttaagt acaagcaacc
      181 gtataattca aataacaatc aacgtctgta tttatccaaa gaatagcttt gtctaaaaag
      241 aagagtatct ttgtctaaaa aagaatagat gcgctttatt gttgacttat tatattaaat
      301 ctaatattta cttataacaa gaagtacttg ctaggattta ttttatactt ttttgtttgg
      361 cttagatact aaataattca gttacaaaag caattataag taaattataa tcgagggaat
      421 cattatgaac gtagtaaggg aaatagtaaa acctattaat catgtaataa cgataaactt
      481 acccgaagga ttcacttcaa atgaagaggt agaagttatt atattgccta ttttaaaaga
      541 aaactctaga aaagaaatca gagtagaaaa atttcttaat cttgcaggaa aaatagaaat
      601 tgatgaaaat gctgtaaatg gattacggga aaacagtctg ttatgattct tgttgataca
      661 aatataatta ttgatttttg gaaaaacccc gaaagtaaaa tttgcgatat atttatacaa
      721 gaagatatcg ctacctgcgg tattattctt gccgaactga tacatggtgc aaagtccgaa
      781 aaagagagtg ctataattca agaagcttta tctgcattta actacctgga tattcgcaaa
      841 aatgattggg taggtattgg ttccttaata aactagtatt gtttcaccca taattactct
      901 tgcggtcgcc ggcactcttt aagtacaagc gaccgtataa ttcaaataac aatcaacgtc
      961 tgtatttatc caaagaataa ctttgtctaa aaagtaattt ttcgctatca tcaaaaatca
     1021 gtatcgttgc cttgttaaaa aattcatact tacgcctgta atggcataaa aattcgtacc
     1081 tgaaagccat atagtcgcta aggtgatacg gttttccagt tgcagtattt acaccagcct
     1141 taatgttttt cgggtacttg acaattgtct tttctccatc aatttcatac tcgaagagtg
     1201 accggtcgtc gggaaggctg ctaaagtatc ttgtgtcaat ttttttcggt acaatttttt
     1261 tctcaccgaa actgtcgtat acccaaacca actcttcgtg tgtaactatg atatgaatgt
     1321 aaagatccaa gtctacatcc acctcgtttt ctaactgaaa atccacctgt aaaagttctc
     1381 ctttacctgc cggatcaact ttcttgttaa acgagacgtg acgaatagca aattcatcgc
     1441 tatctatcat ctgagttggg aaatcctttt cagttgaact tccgcttttt ttagtactat
     1501 cttctttttt gtcggccgcc tgagctgtta aaatcattgg aacaagcagc ataatcacta
     1561 ttatcttttt catatcatcc tccgtgtttt ccgggttaat acccgccttc gatacctatc
     1621 tgaagagccg cgccatgata cgcgcgagaa ggcataacgg cctccaacat tattttttcg
     1681 tacaaaacaa aattatcaga aaacctctgc tcaaactttg cacttatcac atgactcagt
     1741 gtacttattc ccgacagagg atctcccttt aaccatttac gggtgtttat accaaacata
     1801 ccctgtccgt ttaagctcga ataggggtta cggttattat aatcactcat atatattctt
     1861 tcgatcaaat gaaggtgtat acggtgaaag gccgaagcgt tgttattaaa aattacaccc
     1921 gcctttactt cataaacacc attataatct gacacaggct cgtcggcctt gttatcaaaa
     1981 aaataaaatt cagatgattt gaaaccaaaa ctaaggtcaa aacttcttct ataagccctt
     2041 acccaatcaa cttcgagcag agctgtgtca agatatcgct cacttccgta tccagtttca
     2101 tgtggaataa gtctcggata ccagcgcata tcaagacgat ttttatcgtt ttcatatcca
     2161 taaacaattc cgattttaaa atcgtgtaaa agcatatcct ttgaaaaatg gtgatcaccc
     2221 tgataatgtg agaccataag cccagcacca atataatgag cttctccaat aatataacct
     2281 tcaactcttt ctgtcagaat aatcgtatcc ggcattaatg tgccgaagta gggattgccg
     2341 acataaagac gtgctcccca gcgattttta tgattgtatt ctccataagc ttcaattgaa
     2401 aaatcagcac taaaaggccc agggttatca agatactcaa tagaatagtc atcagtgctt
     2461 ataccaagtg ggccacaaat ggctactcca gcagtaaaga cattgcgctt tgttggttta
     2521 aatgtacggt tagggtcatc tagcaggtac ttgtttcggc tgtaacgctc tctgtatgcc
     2581 gaagttaacc atgtaggaat ctcctcgtta gtcggatcaa gccgcatgcc ctcttcaccg
     2641 atttcgacag ctttttggtg ttgacccaat ttataactga cgtatcctgc tctttcatac
     2701 gaaactatct gtcgggggtt acgctttatg gcctcttcat aatttatcag tgcatttttt
     2761 aaatcaccct gctgttcata actccagcca aggttgtgat aaaccagtgc ggcaccctcg
     2821 tctgattttt taactccact attaaaatct ttttcggcct cgtaataatt tccgttttta
     2881 cttttctcta cccccgaact gttgtactgc tccacagttt gtgctaacag atagtgcgcc
     2941 cgcaaaagca taatagtcag aagcagggga aacattacac atgatttttt catcttatgt
     3001 accccgctcc aaacctgatt acatattcgt gcctgtccga ctcataacca gctagctcca
     3061 aaagaacact ctgataaata aaaaagtttt ctgaaaacct ctcatcaaac cgtagttgga
     3121 aaattgttgt tttctgatcc tgtccggaaa caagagagcc gttatcacgg tttttatcaa
     3181 tacccagaaa gctctgcccg ttaagaaacg aatatggatc atcaacagtt tttataatgt
     3241 actgacgcaa cccaacctct gcagtaagtt taagatccac acctgggaaa aaactgtagt
     3301 ttgagacagt cgctccaatt gcagcatcaa agaaaccgta ataatccgat aatggatcat
     3361 catgatttat gaaatagtag tcgtaactcg caattcttgt atagtagatg aaatcgggtt
     3421 taaaaagata tgtgtactta aactcgaact gggctgtatc gtaactggtg ccggatagaa
     3481 agctccaatc cagaagcagt aatctgggat aaaaaagaag atccataata tagtcttcac
     3541 cttcgtaatg caaagagcca cccagtttat agtcatgtaa tgtctgcttc ttgtcttgaa
     3601 aaatattacc gtcgtaatgg ttaagaagca gtcctccccc aactacgagt cttcccatat
     3661 aatagaggca ctcaactcgc tcactctgcg aaacaacatc tgcaaatccc aaacctgtgt
     3721 atggatgttc caaactgcca aaaaaaccaa ttttatcgaa tggtctataa taggcctgca
     3781 taagataggg gaaatttgtt aaaattccct cgcttttctg gtaaccgcta accgaatcct
     3841 catctggctg ataaaaaaag cgcaacgaaa aatcgaatgt tccgtagaag ccgcccctgt
     3901 aaggatcaat tctctggtaa gggctttttc cctgccaaat cggatccctg tttacagcca
     3961 cctctttctc ttcggtaggc tcaattgtat ctatcttctt gcggtacgca tcatgaatcc
     4021 agctcttaac ctgccggtta tccggatcaa tttccagagc cttctcacca taggtaactg
     4081 ccttggaata tcttccctgt ttataattca gatagccaag ccgctcaaaa gatacagcct
     4141 ggcggggatt acgtctaaca gcctcttcaa aagcagctat tgcgtcatca atattgccgc
     4201 gtttttcaaa cattacgcct ttgttatgca aaagacgagc cgattcacgg ttataaatgg
     4261 aaacagcacg cgagaaatca tcacgcgctc tttcatgtag cccgtcctta gccctttcga
     4321 ccccgcgttt attaagcgaa tcacttgtct gggcagacaa aacaggcgat gcacaaaaca
     4381 tctgtactac gaacagtacg gcaatcactg taaatttgac tattttcaca tcagtacact
     4441 taaacttgta ttttttcatg gccacggaac ctgttttatg cccgctataa agagtatcga
     4501 ccctttttaa gaattgtttt atagaaaact tcgaaaaatt aaccatgaaa aacagtgtca
     4561 aaacatttta ttaatggtta taatgacatt ttttaagccc aatcggatca ataatcacca
     4621 taattgaatt gacacataaa gtcgattaca tgtctgttaa tagtgttaac caaaatcacg
     4681 acgtttaaaa attcggttaa tatacaaact aattccattt tactgacttg attacattca
     4741 taatttgttc atacatttta tgtttagaaa caggcaatgc aatgaataac tctcacccaa
     4801 tagcaatcga actatgttcg atagtaaata tccactccgg aaactgctcc gaaaattgtc
     4861 tattttgtgc ccaatcaaga cacaattcct gtgatatcga gaaattcgat tcaattgacc
     4921 ctaataatct tgttaatcac gcgaaaatgc ttcaggctca tggagtaaaa tatatatccc
     4981 ttgttgcaag cggacgcgga ccctcaaaag agcttttagc aactgtcacc gctttaacag
     5041 aaaagatact gtctaataca acactacaag tctgctgttc actggggctt ttgacaaaat
     5101 ctgacgccaa ttttctgaaa acagcaggta tcagtcgata ccatcacaac ctagagaccg
     5161 gtgaatcgtt tttccctcaa gtttgctcga ctcatagctt tgatgaccga attcgtacta
     5221 ttgaaaacgc gcgcgaagca ggccttgaaa tctgtttggg tgggatgata aatattggtg
     5281 aaagcatgga tgatcgtata aacatggtcg aaacagcaat aaaaaaaggt gcaacctctg
     5341 tgcctcttaa tgttctttta cctgtagagg gcacacctct ttacggcaaa attttgatgg
     5401 atccggacga gcttatttac accattacta ctctatattc catgttccca gacacaattt
     5461 tccgttttgc aggtggacga agccaatatt cagaagaaat tacgataaaa agcctaaaag
     5521 ccggtattcg gggaatcatg acgggaaact accttacaaa agcaggtatt cctgtggaca
     5581 aggatattaa attaattgga caaaacggat attctgttgc atcttaacag ataattactg
     5641 atgaaaactc acttattata gcagaaaata gtaaaaatac aaaaacaggc aataccgtcg
     5701 aggtgacaat gtttattaaa aactatgaca aagaagccat tatagaaaat ggtgaagaga
     5761 ta
//
LOCUS       DAOTGZ010000179         5724 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_3064
            NODE_16681_length_5724_cov_4.988181, whole genome shotgun sequence.
ACCESSION   DAOTGZ010000179 DAOTGZ010000000
VERSION     DAOTGZ010000179.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868088
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 5724)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5724)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:22:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,785
            CDSs (total)                      :: 2,751
            Genes (coding)                    :: 2,732
            CDSs (with protein)               :: 2,732
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 12 of 19
            Pseudo Genes (internal stop)      :: 5 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5724
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16681_length_5724_cov_4.988181"
                     /isolate="AMR_MDS_3064"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..542)
                     /locus_tag="P5123_10660"
     CDS             complement(<1..542)
                     /locus_tag="P5123_10660"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRX16765.1"
                     /translation="MKAKLIFLMLVLSLFAAQMSADTATSGLVPTVSLKRATFHDVRY
                     IQAEIIVQNRGKEPASFIVYDKPYTTFQPVVYDVNGREAPVRVAYRLTGLRADQVLGG
                     VASRTILLVPGEKYSCSVNLADYYEFAHGREYKVRLLFLRDAAGKEARLGGNIARLSV
                     SRERLAPVQPQVAIDGIEAA"
     gene            787..1779
                     /locus_tag="P5123_10665"
     CDS             787..1779
                     /locus_tag="P5123_10665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018696169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II fructose-1,6-bisphosphate aldolase"
                     /protein_id="HRX16766.1"
                     /translation="MTSYKDLGLVNTREMFAKAVKGGYAIPAYNFNNMEQMQAIIQAC
                     VETKSPVILQVSAGARQYANQTLLRYMAQGAVEYAKELGYNIPIALHLDHGADFEICK
                     ACVDSGFSSVMIDGSHHPYEKNIEVTKKVVEYAHAHDVTVEGELGVLAGVEDDVVAEK
                     STYTKPEEVEDFVSKTGVDSLAISIGTSHGANKFKPEQCTRNADGILIPPPLRFDILE
                     EIEKRLPGFPIVLHGSSSVPQDLVKVINEHGGKMADAVGIPEEQLRKAAKSAVCKINI
                     DSDGRIAMTAAVRKVFVDSPGEFDPRKFLGPARDSLKELYKLKNINVLGSANQA"
     gene            1896..2975
                     /gene="corA"
                     /locus_tag="P5123_10670"
     CDS             1896..2975
                     /gene="corA"
                     /locus_tag="P5123_10670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010852670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015087 - cobalt ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0015095 - magnesium ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006824 - cobalt ion transport [Evidence
                     IEA];
                     GO_process: GO:0015693 - magnesium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium/cobalt transporter CorA"
                     /protein_id="HRX16767.1"
                     /translation="MAKKLTKLIPKKRIHKSGLAPGSAIFTGDVKMENVVITIINYDK
                     TGIKSFSPSTANEAIETASILDGTIWINIDGLHDVKSIENICSFYDIHKLTIEDIVSV
                     NQRPKLEEHGEYIHLVLKMLDMNATDIIDSEQISLICKGNILLSFQERTGDVFDGVRE
                     RINSGKGFIRQRGASYLLYALTDSVVDRYFYILEFIGERLDRVEIGLLDNPEKAYLND
                     LHLLRKEILKVRRAVYPVKDVALQLEKTSTSSFPQELDIFLRDLYDHTVRVIETVELY
                     RDNSSGLLELYMNSVSNRMNEVMMTLTIIATIFIPLTFIAGIYGMNFTNMPELQYKYG
                     YFVILAIMFFLMLGMLAFFKRKKWF"
     gene            3056..3820
                     /locus_tag="P5123_10675"
     CDS             3056..3820
                     /locus_tag="P5123_10675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013388346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease III"
                     /protein_id="HRX16768.1"
                     /translation="MKTIYSWNVNGIRAVQKKGFIDWIKKESPDIIALQETKARKEQL
                     SEELTDINGYSSHFISAQKPGYSGVALYTKETPLSVEPLGIDQFDSEGRTLIASYNDF
                     ILINCYFPNSQEEGKRLDYKLDFCDAILAKCESLVNDGNNIILMGDYNIAHKPIDLKN
                     PKTNEKNPGYLPQERDWMSKFLDRGFADTFRHYHPDEEGHYSWWSYRFKARERNTGWR
                     IDYHCVNNRFLDKVRKASIHADVMGSDHCPVSIEIE"
     gene            complement(3824..4624)
                     /locus_tag="P5123_10680"
     CDS             complement(3824..4624)
                     /locus_tag="P5123_10680"
                     /inference="COORDINATES: protein motif:HMM:NF016777.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HRX16769.1"
                     /translation="MIVDVHAHIFPDKIAIRAIESLAANSGDYKPFGDGTLDNLLINM
                     NEADIDLAFVANIATKPQQFKSIMDWSVSIRSDKILPLGSVHPQSKDYEKEITSLLDN
                     GFCGIKLHPLYQEFYVDDKKILHFFQALEQSGMFVLMHSGNDIAFPGDDNASPARMAR
                     LLESVPSLDIIAAHMGGWDVWDAVLSDLAGANCWFDTSFVSHIDTDLIYKIVQKHGVD
                     KIVFGSDFPWQRQDSQVNFIKTMFDKTEDCDKIFSGNILKLLKKYKVG"
     gene            4915..>5724
                     /locus_tag="P5123_10685"
     CDS             4915..>5724
                     /locus_tag="P5123_10685"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRX16770.1"
                     /translation="MNKIQILTKYFSKKIETHFPGAYTFTVRLCNLLKRKLLDTGFFD
                     FKFGGANIAVTLIQLYYYIYYQFGKDMPRFRFTTDGFIELITSFLSINLLLLFISCLL
                     SKLPRLKAGIDIFIVWLYYQLMVYQAEAKNTLDFTLVFDNIDITFTTEAYEVIFGSLS
                     ISSYIVSLFIVIILLFFQIKKRNLTKSVQKNRVVFKFGFTIVAYLMILFFQIPTRDNI
                     TFFFQSMIRHSMDNVANIKIEKGTYPFFKHNIKKSNNVDLKRHSTDRPSIIF"
BASE COUNT         1865 a         1187 c         1007 g         1665 t
ORIGIN      
        1 tctgcagctt caatgccatc tattgcaacc tgtggctgta caggtgctaa tcgctcacga
       61 gaaaccgaca gacgcgctat attaccgcct agtctcgcct ctttcccggc tgcatcacgc
      121 aaaaaaagta gccttacctt atattcacgg ccgtgagcaa actcgtaata atccgcaaga
      181 tttacactac agctatattt ttcaccagga acaagaagaa tcgttcgtga agcaacccct
      241 cccaaaacct gatccgctct aaggccagtt aatctgtatg caactctaac cggtgcctcg
      301 cgaccattta catcgtagac taccggctga aaagtcgtat aaggcttatc atacacaata
      361 aaagatgccg gctccttccc gcggttttga acaataatct cggcctgtat ataacgtacg
      421 tcatgaaaag tcgctctttt gaggctgact gtaggaacaa gtcctgatgt tgccgtgtcg
      481 gccgacattt gagccgcaaa aagagacaaa accagcatca aaaatatcaa ttttgctttc
      541 ataaaacacc gttttttttc attatgctac ataataatat tatatttatc ggcattttcg
      601 taaaattatg tctaaatttt ctgccgatat gcggatgcta acctattata attacacaca
      661 attccgatgg ccaaactgtt ttatgacaca ccaggctccg aaaaatgcct tgcaattata
      721 cctttatatg taatttttga ccagactgtt catgcgatta atatacaata atataaggag
      781 acattcatga caagttataa agatcttggg cttgtgaaca caagagagat gttcgctaaa
      841 gccgtaaaag gcggttacgc tattcctgct tacaacttca acaatatgga gcagatgcag
      901 gctattattc aggcatgtgt cgaaacaaaa tcacctgtaa ttcttcaggt ttcagccggt
      961 gcacgtcagt acgcaaatca aacacttcta cgatacatgg ctcagggtgc agtagaatac
     1021 gcgaaagagc tcggttacaa tattcctata gcgcttcacc ttgaccatgg tgccgatttt
     1081 gaaatatgca aagcttgtgt tgacagtggt ttctcgtctg taatgatcga cggatctcat
     1141 catccatacg agaaaaacat tgaagtaact aaaaaggttg ttgagtatgc tcacgcacac
     1201 gatgttactg tcgaaggaga acttggtgtt cttgccggtg ttgaagacga tgttgttgct
     1261 gaaaaatcta catacacaaa acctgaagaa gttgaagact tcgtatcaaa aacaggagtt
     1321 gactctcttg ctatctctat aggaacttct catggtgcaa acaagtttaa acctgaacag
     1381 tgtacccgta atgccgatgg cattcttata ccacccccac ttcgttttga cattcttgaa
     1441 gaaattgaaa aaagattacc aggattccca atagttcttc atggatcgtc ttccgttcca
     1501 caggatcttg ttaaagtaat aaacgaacat ggcggaaaaa tggctgatgc agttggcatc
     1561 cctgaagagc agcttcgaaa agcagctaaa tctgcggttt gcaaaatcaa tatcgactct
     1621 gatggccgca tcgccatgac agctgctgtt agaaaggtat ttgtagattc tcctggagaa
     1681 tttgaccctc gcaaattctt aggacctgct cgtgacagtc ttaaagagct ttacaaactt
     1741 aaaaacatca acgttctagg aagcgctaac caagcttaag aattccataa atatacaata
     1801 aaaaaggccg gatattgtta tccggtcttt ttttcttctt gcacgatatt tttcacggtt
     1861 tatcaaagac aaaactggag aaagcataaa tatttatggc aaaaaagcta acaaaactga
     1921 tacctaaaaa acggattcat aaatcaggac ttgctcccgg ttcagcaatt tttaccggcg
     1981 atgttaaaat ggaaaacgtc gtcataacaa taataaacta tgataaaaca ggtattaaaa
     2041 gcttttctcc gtcaaccgca aatgaagcca ttgaaacagc ttcgattctt gatggcacta
     2101 tctggataaa tatcgacggc ttacatgatg taaaatcaat cgagaacatc tgctcatttt
     2161 atgatattca caagcttacc atagaagaca ttgtaagcgt caatcaacgt cctaaactgg
     2221 aagagcatgg cgaatatatt catcttgttc ttaaaatgtt agacatgaac gcaactgaca
     2281 ttattgactc ggagcagatc agtttaatat gcaaaggcaa cattttacta tcgtttcaag
     2341 aaagaaccgg agatgtcttt gatggtgtaa gagaacgcat taattcaggc aagggattca
     2401 ttcgtcaacg cggagcctca tatcttcttt atgcgcttac cgattcagtt gttgaccgct
     2461 acttctacat acttgagttt atcggtgaac gtcttgatag agtcgaaatc ggactgctag
     2521 acaatcctga aaaggcctat ctgaatgatt tgcaccttct acgaaaggaa attcttaagg
     2581 ttagacgagc agtgtatccg gttaaagatg ttgctcttca gcttgaaaaa acaagcacct
     2641 cgtcttttcc gcaagaactg gatatatttt tacgagatct gtatgaccac acagtacggg
     2701 ttatcgaaac tgtcgaactt tatcgcgaca actcttccgg tttgctggaa ctttatatga
     2761 acagcgtctc aaatcgtatg aatgaagtca tgatgactct aactattatt gctaccatat
     2821 tcataccact cacatttatt gccggaatat acggcatgaa ctttacaaat atgcccgaac
     2881 ttcaatataa atatggatac tttgttatac tggccatcat gtttttttta atgctcggca
     2941 tgcttgcctt ctttaaacga aaaaaatggt tctaacaaac acaaggaacc tcgattattt
     3001 ggcaaatagc tttttttgaa taattacagt cagctcaaaa atgaggaaag cacgaatgaa
     3061 aaccatctac tcctggaacg taaacggtat acgcgccgta caaaaaaaag gttttatcga
     3121 ttggataaaa aaggaatctc cagacataat tgccctgcaa gagacaaagg cgcgaaaaga
     3181 acagctttca gaagagctaa cagatattaa tggatattca agccatttca taagtgccca
     3241 aaaaccaggt tatagtggag tcgccctcta tacaaaagag actccccttt ctgtcgagcc
     3301 tctcggaata gatcaatttg atagcgaagg ccgaacactt atagcgtcgt acaacgattt
     3361 tattctgata aactgctact ttccaaacag ccaggaagaa ggcaaaagac tggattataa
     3421 acttgatttc tgtgatgcca ttctagcaaa atgtgaaagc cttgttaatg acggcaacaa
     3481 cattatcctt atgggagatt acaatatcgc acacaagcct atcgatttaa agaaccctaa
     3541 aaccaatgag aaaaacccgg ggtatcttcc acaggaacgt gattggatga gcaaatttct
     3601 tgaccgtggt tttgccgaca cctttcgcca ttatcatcca gacgaagagg ggcactacag
     3661 ctggtggtct taccgtttta aagctcgtga aagaaataca ggctggcgta tcgactacca
     3721 ttgtgtaaac aacagatttt tggacaaagt caggaaggca tccatacacg cggatgttat
     3781 gggctctgat cactgccctg tgagcataga aatagagtaa aaatcagcca actttgtatt
     3841 tctttaaaag cttaagaata tttcctgaaa aaattttgtc acaatcttca gttttatcga
     3901 acattgtctt gataaaattg acctgactat cttgtcgctg ccatggaaaa tcactaccaa
     3961 aaacaatttt atctacacca tgcttttgga caattttgta aattagatcc gtatcaatat
     4021 gagatacaaa agaagtatca aaccagcaat ttgcgccggc cagatcagac aaaactgcat
     4081 cccaaacatc ccatcctccc atatgagccg cgataatatc caaagacggc acactttcaa
     4141 gcaaacgagc catccgggca ggagaagcgt tatcgtcacc aggaaaagct atatcattac
     4201 ccgagtgcat aagtacgaac atgccactct gctccaaagc ttgaaaaaaa tgtaatattt
     4261 tcttatcatc gacatagaat tcctgataaa ggggatgaag ctttattccg caaaaaccat
     4321 tatccaaaag agatgttatc tctttctcgt aatccttgct ctgtggatga accgatccca
     4381 aaggtaaaat tttatcagat cgaatagaga cagaccagtc cattatcgac ttaaactgct
     4441 gcggcttggt ggcaatattc gcaacaaacg ctaaatcaat atccgcttca ttcatgttaa
     4501 tcaaaaggtt atcgagcgtt ccatctccaa aaggtttata atctcctgag ttagccgcaa
     4561 gtgattcaat ggcgcgaatg gcaatcttgt ctggaaatat atgagcatga acatcaacaa
     4621 tcatatgtac acctatcacg gattataccg ttgcctattg tttagttttc tgttgaaata
     4681 attgaattat aaattctcaa aaatcaactc aatacttgga atatcttttt tacaaacttt
     4741 ttatatagtt tgataattat caatataaaa acaataatcc ttttatatta actggctaac
     4801 aatccacgaa aacggctata caaactatac ctctaccaaa atctttcttg acacggcaat
     4861 tctcaacaat cccattatca ctatatcagt caaacaggat acttataata atatatgaac
     4921 aaaattcaaa tactgaccaa atatttttct aaaaaaatag aaactcattt tccaggtgca
     4981 tacacattca ctgttaggct ctgtaatctt ttgaaaagaa aattattgga tacaggcttt
     5041 tttgatttta agtttggcgg cgccaatatt gccgttaccc tgattcaact ttattattat
     5101 atctattacc agtttggcaa agatatgccg cgattcagat ttacgactga tggttttatt
     5161 gaacttatca ccagttttct ttcaattaat cttcttctcc tgtttataag ctgccttcta
     5221 tcgaaacttc cgagactaaa agcaggtata gatattttta tagtatggct ctattaccag
     5281 cttatggtct atcaggccga agcaaaaaat accctcgatt ttactcttgt ttttgacaat
     5341 attgatatca catttactac agaagcctat gaagtcatat ttggttcgct tagtatatct
     5401 tcttatattg tttcactgtt tattgtaatc atattactgt tttttcaaat caaaaaaaga
     5461 aaccttacga aaagcgttca aaaaaacagg gttgttttca aatttggttt tactattgtt
     5521 gcatacctta tgattctttt tttccagata ccgacccgcg acaatatcac attctttttt
     5581 caaagcatga taagacatag catggacaat gtagccaaca taaaaataga aaagggaacc
     5641 tacccgttct ttaaacataa cattaaaaaa tcaaacaacg tcgatttgaa aagacattcg
     5701 acagacagac cctcaattat tttt
//
LOCUS       DAOTGZ010000180         5700 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_3064
            NODE_16772_length_5700_cov_4.797343, whole genome shotgun sequence.
ACCESSION   DAOTGZ010000180 DAOTGZ010000000
VERSION     DAOTGZ010000180.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868088
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 5700)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5700)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:22:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,785
            CDSs (total)                      :: 2,751
            Genes (coding)                    :: 2,732
            CDSs (with protein)               :: 2,732
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 12 of 19
            Pseudo Genes (internal stop)      :: 5 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5700
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16772_length_5700_cov_4.797343"
                     /isolate="AMR_MDS_3064"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            365..754
                     /locus_tag="P5123_10690"
     CDS             365..754
                     /locus_tag="P5123_10690"
                     /inference="COORDINATES: protein motif:HMM:NF013868.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043856 - anti-sigma factor antagonist
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="STAS domain-containing protein"
                     /protein_id="HRX16771.1"
                     /translation="MECKVIKSGSDVTEAEIKGDCTIYNAAEIKKFISKYYNKSDSLV
                     LNLSGVDEIDSSFFQLLLALKKDARENNKRVSFIAHSIPVLKYIDLYGAAAMFGDRLV
                     VKKEDREKFDFKYGLKTVKGASGNESR"
     gene            741..2981
                     /locus_tag="P5123_10695"
     CDS             741..2981
                     /locus_tag="P5123_10695"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis protein CheA"
                     /protein_id="HRX16772.1"
                     /translation="MNLDSAKKVFFLESRENLQRIEENLLTLLENAHDQEFINALFRE
                     VHTIKGSAGMFGYQDVTNFAHVFENLLDGVRNGEVGVSDQLVEVSLASHDYMKNLIDF
                     AENEKAVPDEVVSEGEVLIERLKAFQSETEKKEKQKAAPINYESVESRKPQTSTSDTS
                     SGVENRYWHISLRFGENIFTTGLDPFSFIGYLKNIGTIVNVIPVTQQIPEFKDFNPEI
                     CYIGFELDFDADKGIDKGKIMDVFEFLTDDCTVIVLPPHSEVDHYCQMIADLPESAVQ
                     IGDILTQSGTLTKAELEEALELQSSKSLDDGEKHPLLGEVMLKEKMIPETVMEAALEK
                     QKENRAKEEQRKRSIRIDADKVDQLITLVGELVISGANIKQRIGQTDDTDLSEAVEQM
                     ARLVDDVRDSTMYIRMVPIGETFSTFKRTVHDLSRDMGKQVELVINGGDTELDKTLVE
                     KIQDPLMHIVRNAVDHGIESPEERLAMGKKEKAVIRLNAFHESGSIVIEVSDDGKGLS
                     RDKIYAKAVVNGLVTSGQNVDDRTLWNMIMLPGFSTAEKVTDISGRGVGMDVVKKNID
                     SLRGVVDIESTEKQGTTIRIHLPLTLAIIDGFLVTVANQKYVFPLDMVLECTDVEFNQ
                     DDKEGGNFFTLRGEVLPYLSLGELFYGDNFKNIDKTNGNRVFSHDDKNKIVIVEYARK
                     RAGFVVDHLLGELQTVIKPLGKLFYNMHWISGATILGNGEVALIIDVPRLIEYVGNNV
                     KLRGKK"
     gene            2978..3499
                     /locus_tag="P5123_10700"
     CDS             2978..3499
                     /locus_tag="P5123_10700"
                     /inference="COORDINATES: protein motif:HMM:NF013731.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis protein CheW"
                     /protein_id="HRX16773.1"
                     /translation="MKEKRIYEKEQHENRTMVQLLSFRQGREIYAIKIHSVREVIDSI
                     AITPIPLVPLYIKGVINLRGNIIPVVDLNARFYGDKGEIVKTTSIIVIEVPFEEELVT
                     IGLMIDAVKEVLMFPEEEIDPTPDFGTNIRTDYISGVARHEDNYLIILNVNSVLDIAE
                     LSDFTCEAAIVGE"
     gene            3501..4604
                     /locus_tag="P5123_10705"
     CDS             3501..4604
                     /locus_tag="P5123_10705"
                     /EC_number="3.1.1.61"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018150987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0008984 - protein-glutamate methylesterase
                     activity [Evidence IEA];
                     GO_function: GO:0050568 - protein-glutamine glutaminase
                     activity [Evidence IEA];
                     GO_process: GO:0006482 - protein demethylation [Evidence
                     IEA];
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA];
                     GO_process: GO:0018277 - protein deamination [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis response regulator protein-glutamate
                     methylesterase"
                     /protein_id="HRX16774.1"
                     /translation="MDLINVAVVDDSALVRQVISELLDNKYGIKVSFKAPNPIIAERY
                     MDKEWPDVIILDIEMPEKDGLTFLKEIMEKRPTPVIICSGVTEKNARVTIEALSSGAF
                     SIITKPKVGMKDFFLESEQLLADEVRAAYNSNRAILKKKTPAVEPVSRLIEKRYTADV
                     VLSAPVKKVDVSGLDRIVVIGASSGGTQAIEAVLSELPKNCPGIAIVQHMPEKFTKAF
                     ADRLNSICNIEVKEAEQDDQIIEGRAIIAMGGKHMSLKKSGNSYKVSILDGPLVSRHR
                     PSVDVLFRSAAKYGGSNILGIILTGMGDDGAAGLLEMKQAGCYTVAQDKESSLVYGMP
                     RVAFERGAALNVVSLGVVPDTIMKFVYAGDEGF"
     gene            4657..>5700
                     /locus_tag="P5123_10710"
     CDS             4657..>5700
                     /locus_tag="P5123_10710"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRX16775.1"
                     /translation="MIAENGKADIDNDISQLEFFSGEIRDNASRINRSTILRKENAQE
                     IIERVKRAMIFSTSIHDDIVQVAAANTMWLNKHNSIYSSCKMLKANIAEQRYCLDLLK
                     RENLINNDDLKTIEESLVSFDNALQSALACLNDVLDKNNDIILLDKILELKKRYQLDS
                     LKTLKKLALAVYIDSETAEKGSLINFSRWTEFYDSIEIIKESFINQDTDKLEHFMTTI
                     RKAGNQTEEVIINSKSQYEFNEQVNHFMERFYNESISIKEVIDKKHSFFEQNLQNSTV
                     LTVIISFEFKKYLELEKTLNSISISFDAPSDIRGIYADLMLLNEIASNDIKVLTELNL
                     DMTEISHITNENET"
BASE COUNT         1848 a          869 c         1286 g         1697 t
ORIGIN      
        1 taggccaaca agcataggga tatgagtttt ccgacaaata tgtaactgca tagagcctcc
       61 tgagttaaac gttatacctt aaaaaaacag ttattgcata tttaattaag gaatagcggc
      121 ttaaaaattt ggagctttat ttaaagttat cttgccatgc cattagtttg agtgtatact
      181 attaaaagaa ttttgcaaaa cctgagtgtc gaatttttca gtaccatatt gtgtgaaaca
      241 atatatcatt tgaaattgtt ttgtaattaa atacatataa agtttttgct ttggtcaaca
      301 agttttattc aaaaaaacaa tgatggatga cttatgtttt aggctgtctg tcggggagaa
      361 tggtttggaa tgtaaggtga tcaagagtgg ctctgatgta acagaggccg aaataaaagg
      421 tgattgtacc atatataatg ccgcagaaat taagaaattt atcagtaaat attataataa
      481 atcggactct ttggtattga atctgtctgg agtagatgaa attgactcgt cgttttttca
      541 gcttctttta gctctaaaaa aagatgcgag agagaataac aaaagggtca gttttattgc
      601 gcattcaata cctgttttaa aatatatcga tctttatggt gcagcagcga tgtttggtga
      661 cagattggta gttaaaaaag aggatcgtga aaaatttgat tttaaatatg gtttaaaaac
      721 tgttaaagga gcgtcaggaa atgaatctcg atagtgctaa aaaagttttt tttctggaaa
      781 gccgggaaaa tcttcaacga attgaagaga atctgcttac gcttcttgaa aatgctcacg
      841 atcaggaatt tataaatgct cttttcaggg aagttcatac catcaaaggg tcggcaggaa
      901 tgtttgggta ccaggatgta acaaattttg cccatgtatt tgagaacctg cttgatggtg
      961 tacgtaatgg tgaagttggt gtaagtgatc aattagtaga ggtttcactc gcgtctcatg
     1021 attatatgaa aaacctgatt gattttgctg aaaatgaaaa agctgttcct gatgaggttg
     1081 ttagtgaagg agaggttctg attgagcgtt taaaagcttt tcagtcggaa acagaaaaaa
     1141 aagagaaaca gaaagccgcg ccaataaatt acgagagtgt tgagtcccga aagccgcaga
     1201 cttcgacgtc tgatacctca tctggagtcg agaataggta ttggcatata tcactccgtt
     1261 ttggagagaa tatttttaca acaggccttg atcctttttc ttttatagga tatttaaaaa
     1321 atataggtac cattgtaaat gtgataccag tgacacaaca aatacccgag tttaaagatt
     1381 ttaacccaga aatatgctat ataggttttg agctcgattt tgatgcagat aaaggcattg
     1441 ataagggcaa gataatggat gtctttgaat ttttaaccga tgattgtact gttattgtac
     1501 tgcctccaca ttccgaggtg gatcactact gtcagatgat agcagactta cccgaaagcg
     1561 cagttcagat aggtgatata cttactcaat ccggaacttt gacaaaggcc gagcttgaag
     1621 aggcgcttga gctacagagt agtaaatctt tagatgatgg tgaaaagcac ccactgttgg
     1681 gcgaagttat gttgaaagaa aaaatgatac ccgaaacggt aatggaagcg gctcttgaaa
     1741 aacagaaaga gaaccgggcg aaagaagagc aacgaaaacg ctctattcgt attgatgccg
     1801 ataaggttga tcagcttata acacttgtcg gtgaactggt aatatcggga gcaaatatta
     1861 aacagcgaat aggtcaaact gatgatactg atttgtcaga ggctgtcgag cagatggcga
     1921 ggcttgtgga cgatgtcagg gattcgacta tgtacattcg aatggttcct ataggagaaa
     1981 cgttttcgac gtttaaaaga acagttcatg acctttcacg tgatatgggt aagcaggtag
     2041 aacttgttat aaacggtggt gataccgagt tggataagac tcttgtcgaa aaaattcagg
     2101 atccactgat gcatattgtt cgtaatgcag ttgatcatgg cattgagtct ccggaagagc
     2161 gtttggccat ggggaaaaag gagaaggctg taataaggct taatgctttt catgagagcg
     2221 gaagtattgt tatagaggtc tctgatgatg gaaagggatt gtctcgtgac aagatttatg
     2281 caaaggctgt ggtcaatgga cttgttacgt ctggacaaaa tgttgacgac cgtacccttt
     2341 ggaatatgat aatgttgcct ggtttttcta ccgccgagaa agttacagac atatcggggc
     2401 gcggtgtggg tatggatgtt gttaagaaaa atatcgattc cttgcgtggt gttgtagata
     2461 tagaatcgac agagaagcag ggaaccacaa taagaattca ccttccattg acactggcga
     2521 taattgatgg ttttctggtt acagttgcaa atcagaagta tgtctttccg ttagatatgg
     2581 ttcttgaatg tacggatgtc gaatttaatc aggatgacaa ggaggggggt aatttcttta
     2641 cattaagggg agaagtttta ccctatcttt cgcttggtga gcttttttac ggagataact
     2701 tcaaaaatat cgataaaact aacgggaaca gggttttttc gcatgacgac aaaaacaaaa
     2761 tagttattgt agagtacgcg agaaaacgag ccggatttgt ggtagatcat ctgcttggtg
     2821 aattacagac cgttattaaa cctttgggta agctcttcta caatatgcac tggataagcg
     2881 gagctacaat acttggtaat ggtgaggttg cgcttattat tgatgtgcct cgtttaattg
     2941 aatatgtggg taataacgtt aagctcagag gtaaaaaatg aaagagaaaa gaatttacga
     3001 aaaagagcag cacgaaaacc gcacaatggt tcagcttctc tcttttcgtc agggaagaga
     3061 aatttacgcg ataaagattc actctgttcg tgaagtgatt gattctatcg cgattactcc
     3121 aattcctctt gtgccattat atataaaagg tgtaattaac cttaggggaa atataattcc
     3181 ggtggttgat cttaacgccc gattttatgg tgataaaggc gagattgtca agactactag
     3241 tataattgtt atagaagttc cgtttgaaga ggagttggtt actattggcc ttatgataga
     3301 tgcagtcaaa gaggttctta tgtttccgga agaggagatt gacccgactc cggattttgg
     3361 aacgaatatc agaaccgatt atatttcggg agtagcaagg catgaagata attatctgat
     3421 aattttgaat gtgaattcag ttcttgatat tgccgagctg tcagacttta catgtgaagc
     3481 agctattgtc ggagagtaag atggatttaa taaacgttgc tgttgttgat gattcagctc
     3541 tggttcgtca ggtaatatcc gagctgcttg ataataagta tggtattaaa gtaagtttta
     3601 aagcaccgaa tccaattatt gccgaaaggt atatggataa agagtggcct gatgttataa
     3661 tacttgatat cgagatgcct gaaaaagacg gactgacctt tttaaaagag ataatggaaa
     3721 aaaggcctac gccggttatt atatgttcag gagtaaccga aaagaatgcc agagtgacaa
     3781 ttgaagcatt atcctctgga gcattttcga ttattacaaa acccaaggtc ggcatgaaag
     3841 atttctttct ggagtccgag cagcttcttg ctgacgaagt tagagccgct tacaattcta
     3901 accgtgcaat acttaaaaag aaaaccccgg cagtagagcc agtttcacgt ttaattgaaa
     3961 aacgttatac ggctgatgtt gtactttcgg ctccggtaaa aaaagtcgat gttagcggcc
     4021 ttgacagaat agttgtaatc ggtgcatcat cagggggtac ccaggctatt gaggctgtat
     4081 tatccgagtt gcctaaaaat tgtccaggaa ttgcaatagt tcagcatatg ccggagaagt
     4141 tcacgaaagc ttttgctgac cgtcttaatt cgatatgtaa tatcgaagtc aaagaggccg
     4201 aacaggatga tcagataatc gaggggcgtg ctattattgc gatgggtggc aaacatatgt
     4261 ctttgaaaaa gtctggaaat tcttacaagg tgtccatact agatggtccc ttggtaagcc
     4321 gacaccgccc atctgttgat gtgctttttc ggtcagccgc taagtatggg ggatcaaata
     4381 ttttgggaat aatattaact ggtatgggtg atgacggtgc cgccgggctt ttagaaatga
     4441 aacaagcggg ttgctatact gttgcgcagg ataaagagag ctcgcttgta tatggtatgc
     4501 caagagtcgc tttcgagaga ggagccgctc ttaatgttgt atctcttggt gttgttcctg
     4561 atacaataat gaagtttgta tatgcaggtg atgaaggttt ttaatatttt ttgtatttta
     4621 gcacaataaa tatatatgtc cgagtggaga atcagaatga tcgcagaaaa cggtaaagca
     4681 gatattgata atgatatatc ccagctggag tttttttcag gagagattcg tgataatgct
     4741 tcacgaataa atcgttcaac catattaaga aaagagaatg ctcaagagat aattgagcga
     4801 gtaaaacgag caatgatttt ttcgacaagc atacatgatg atatagtgca ggtagctgct
     4861 gcaaatacta tgtggttaaa taagcataat tccatttact cgtcatgcaa aatgttaaaa
     4921 gctaatatag ccgagcagag gtactgtctt gatcttttaa aaagagaaaa tctgataaat
     4981 aatgatgatt taaaaacaat agaggagagt cttgtctctt ttgataatgc ccttcaaagc
     5041 gctcttgcct gtcttaatga tgttcttgat aagaataatg atataattct tcttgataaa
     5101 attcttgaac ttaaaaaaag gtatcaactc gattcattga aaacacttaa aaaattggca
     5161 cttgccgttt atatcgattc tgaaaccgca gaaaaaggat cacttataaa cttttcacgg
     5221 tggacagaat tttacgattc tatagaaata ataaaagaat cgtttataaa tcaggatacg
     5281 gacaaacttg aacattttat gacgaccata cgcaaggctg gtaatcaaac agaagaagta
     5341 attattaatt caaagtcaca atatgagttt aacgagcagg taaaccattt tatggaacgt
     5401 ttttataacg agtccatatc aataaaagag gttattgata aaaaacattc cttctttgag
     5461 cagaatctac aaaacagtac tgttttgaca gtaataatat catttgaatt taaaaagtat
     5521 cttgaacttg aaaaaacact aaattctatc tccatatcgt ttgatgcacc atctgatatc
     5581 aggggcattt atgccgactt aatgttgtta aacgaaatag cttcaaacga tataaaagta
     5641 ttaaccgaac ttaatcttga catgacagag ataagccata taactaacga aaatgagaca
//
LOCUS       DAOTGZ010000181         5686 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_3064
            NODE_16814_length_5686_cov_4.857752, whole genome shotgun sequence.
ACCESSION   DAOTGZ010000181 DAOTGZ010000000
VERSION     DAOTGZ010000181.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868088
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 5686)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5686)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:22:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,785
            CDSs (total)                      :: 2,751
            Genes (coding)                    :: 2,732
            CDSs (with protein)               :: 2,732
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 12 of 19
            Pseudo Genes (internal stop)      :: 5 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5686
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16814_length_5686_cov_4.857752"
                     /isolate="AMR_MDS_3064"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..347
                     /locus_tag="P5123_10715"
     CDS             <1..347
                     /locus_tag="P5123_10715"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRX16776.1"
                     /translation="AKNPVCNDSLNKYHERGTIYKSVHIRFSSISKRKLKDIANLYCY
                     SLSYMLFLAMDFFDEFMVGVKEFVRPCFHAAFVHHFFGFPYFINSWGMNFYEGQDQPA
                     GQESGKIPILLC"
     gene            complement(419..1096)
                     /locus_tag="P5123_10720"
     CDS             complement(419..1096)
                     /locus_tag="P5123_10720"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013700.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M23 family metallopeptidase"
                     /protein_id="HRX16777.1"
                     /translation="MKVKLFLAVFVLTFLTVSCISQQNKPSKTIVDIAPDNPYLCQGY
                     YHSDKSPYILPFSIGMEYKLVQGNCTRYSHKGFSRYAYDFGMPIGSEIIAARSGVVTF
                     VETDMYDNTRRGKRRHKRIPNARGNTILIEHEDGTIAAYFHLKYNGALVQKGDLVKQG
                     QPIALSGSSGFTTGPHLHFEVRRSNEDRQSLPVTFRNADPGEDFSLQAGNYYTALPFE
                     QDKFASK"
     gene            complement(1160..1244)
                     /locus_tag="P5123_10725"
     tRNA            complement(1160..1244)
                     /locus_tag="P5123_10725"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1208..1210),aa:Leu,seq:gag)
     gene            1317..3176
                     /locus_tag="P5123_10730"
     CDS             1317..3176
                     /locus_tag="P5123_10730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008277968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphorylase"
                     /protein_id="HRX16778.1"
                     /translation="MENNFQSAPSYLNEPDYRNKQLLVDPLFRKKIFKRMTFLYGEYT
                     AKQFYPEMERIIRVHYSYKSDSLIEKEIQERNSDIFSEKDVILITYGDLLKGKDQSPL
                     STLAKFCDIYLEGAINTIHILPFYPYSSDRGFSIKDFETVDPNLGCWEDIHRIGNKYK
                     LKFDGVINHISSKSKWFQEFLNGNPRFQDFVISYDSPDDISEEEKKMIFRPRTSDILS
                     KVYTINGEKYVWTTFSEDQIDLNYKNPEVLIKVIEILLMYVRHGAKIIRLDAVTYIWA
                     ETGTRCVHLDQTHEIVKLFRDVLDFAAPGTLIITETNVPHEENISYFGNGEDEAHMVY
                     NFALPPLVLHTMYTMDSTALSKWASTLDTCSNNTTYFNFLDSHDGIGLMAVKDILSPN
                     DIDLILDNVRQHGGYISYKTGENEKEIPYEMNITWFSAINNTNNPENNDIAYQVKKFV
                     ASRSIALVLQGIPGIYLHSLIGTVNDIDAVVSSKANRDINRTIIDEQEISYALGDGLS
                     KISRINREYGRIIDIRTKSRAFHPNSKQKVVFKSESLFSVIRFTETKDNIIFTIVNIS
                     NETVHTLLTNNELEEISKNWIDLISGMPYSCDDAGLHLVLQPYDILWLSPEKL"
     gene            complement(3179..4060)
                     /locus_tag="P5123_10735"
     CDS             complement(3179..4060)
                     /locus_tag="P5123_10735"
                     /inference="COORDINATES: protein motif:HMM:NF020252.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HDOD domain-containing protein"
                     /protein_id="HRX16779.1"
                     /translation="MALDLPDLKKILYSRVNELPTLPVVIPKILQMMEDNKSDVKKIS
                     EVISYDPALVSKILKVANSSYYGFPKKISEIDRAVSLLGFNMVKSLAVSLGVVNTLSE
                     PRSKRFFSETGLWFHSVAVATLTRELCLNIIKTKKKDYLFVAALLHDIGKLVFDIFFS
                     DLFEQAVLDVQNSGTYSLAKSEKKIIGVDHGEAGALLLKRWNFPDEISDPVMYHHSQD
                     IPSNVSSEDMSILRICDALCQEFFYESIDDIPDPRISETDLENIAIKNSDIKKLEDFL
                     HNNKQNIADFFQSIYQV"
     gene            complement(4074..5249)
                     /locus_tag="P5123_10740"
     CDS             complement(4074..5249)
                     /locus_tag="P5123_10740"
                     /inference="COORDINATES: protein motif:HMM:NF019604.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HRX16780.1"
                     /translation="MKYCIIGNGAAGTDAALGIRTKNEEADIEIFTSSNHHLYYRPKL
                     VEYVGSQMKPEDLILHNEDLYIQKKIKVNLNCKIVSIENDKKIILDSKGKEHKYDFLI
                     IATGARNFLPPVEGSNLKGVFSLRNFGDADLINSYAESVKKIVIAGGGLLGLETANSL
                     LRVCKDVTVVEIADNLLPRQLDDQGGGFLKQLLIKKGLKFICGTSVKQINGNEKIESL
                     TLSTGEKIEADMLVFSAGIRPEIDLALSSGIKCDKGIVVNNHLETSVKGIYAAGDCIQ
                     MEGKLFGLWISAKEQGKTAGLSACGMSETYKGSYPPVVLKITGIDLFSAGYDPLSEEF
                     ANAEKKVVINDSVYKKIYYKNGKALATMIIGDSSAALMARKLMSGTLSLDEFLNTMK"
     gene            5402..5560
                     /locus_tag="P5123_10745"
     CDS             5402..5560
                     /locus_tag="P5123_10745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005658603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rubredoxin"
                     /protein_id="HRX16781.1"
                     /translation="MEKYVCTVCGYEYDPEIGDPDSGIKPGTSFEDIPDDWVCPVCGV
                     GKDQFEPA"
BASE COUNT         1847 a         1104 c          916 g         1819 t
ORIGIN      
        1 tggctaagaa tcctgtatgt aatgactctc tgaacaagta tcacgaaaga ggcacgattt
       61 acaaatcagt gcatatccgg ttttcaagta tttctaaaag gaaactgaaa gacatcgcta
      121 atctgtattg ttacagcttg tcgtacatgt tgtttcttgc aatggatttc ttcgatgagt
      181 ttatggttgg tgttaaggag tttgtgagac cctgctttca tgctgctttt gttcatcact
      241 tctttggctt tccctacttc ataaacagct ggggaatgaa cttttacgaa ggacaagacc
      301 aacctgctgg acaagaatcc ggaaaaatcc caattttgtt gtgttaaaca gaacatattt
      361 tgttactatt gacatttaaa cagaaaaaag taacaaaaat ctggaaacaa aataaaaatt
      421 attttgaagc gaacttatct tgttcaaatg gtaatgccgt gtagtaattt ccagcctgaa
      481 gactaaaatc ttcacctgga tctgcattcc taaaagttac aggcaatgat tgtcgatctt
      541 cattgcttcg tctaacttca aaatgcagat gaggaccagt tgtaaatcca ctgctgccac
      601 ttaaggcgat aggttgcccc tgtttaacta aatcaccttt ttgaaccaga gcaccattat
      661 atttaagatg aaaataggct gcgatagttc catcctcatg ttcaatcaat atcgtatttc
      721 cacgagcatt aggaattctt ttatgtctac gctttccacg tctggtatta tcgtacatat
      781 ccgtctcaac aaaagtaaca acccccgaac gtgctgcgat aatctcactt cctataggca
      841 taccaaaatc gtacgcgtag cggctaaacc ccttatgtga ataacgtgta cagttaccct
      901 ggactagttt atactccatt ccaatagaaa aaggtaatat gtatggagac ttatcagaat
      961 ggtaataccc ctgacacaaa tatggattat caggagcgat atcgacaatt gttttagacg
     1021 gtttattttg ttgcgaaata cagctaactg ttaaaaaagt taatacaaaa acggcaagaa
     1081 acagcttgac tttcatgatt atttccttaa aattactagc aggcaaatac gatcggagat
     1141 aatttatttt ttagtttatt gctcgggacg agacttgaac tcgtacgaac ttatcgttca
     1201 caaggccctc aaccttgcgt gtctaccaat ttcaccaccc gagcatttta gtagctttgt
     1261 acatttatta tttttgggca ctatttgtca agttttttca tatcggcatg gacatcatgg
     1321 aaaacaactt tcaatcagca ccatcgtatt taaatgaacc cgattaccgc aataaacaat
     1381 tgttggttga ccccctgttt agaaaaaaaa tatttaaacg catgactttt ctttacgggg
     1441 aatatacagc caaacagttt tatccggaaa tggaaagaat tataagagtc cactattcat
     1501 acaaatccga tagtcttatc gaaaaagaga tccaggaacg taacagcgat attttttctg
     1561 aaaaagatgt gatactaata acttacggtg atcttttaaa aggcaaagat caatcacctc
     1621 tatcaacact tgcaaaattc tgtgatatat atcttgaagg ggcaataaat accattcaca
     1681 ttctcccttt ttatccttac tcgtctgata gaggattttc cattaaagat ttcgaaactg
     1741 ttgaccctaa tcttggttgc tgggaggaca ttcaccgtat tggaaacaaa tataaactaa
     1801 agtttgacgg tgtgataaat cacatatcgt caaaaagcaa atggttccaa gaatttttga
     1861 atggtaatcc acgtttccaa gatttcgtta ttagctacga ttctccagat gatatttcag
     1921 aagaagaaaa aaaaatgata ttccgacctc gcaccagtga catcctctca aaagtatata
     1981 caatcaacgg cgaaaaatat gtctggacta ctttttcaga agatcaaata gatcttaatt
     2041 acaagaaccc tgaagtacta ataaaagtta tagaaatact tttaatgtat gttagacacg
     2101 gggcaaaaat aatccgtctt gatgcggtta cctatatatg ggctgagacc ggaacgcgat
     2161 gtgttcatct tgatcagact catgaaattg taaaactttt tagagacgta ctggacttcg
     2221 cagcccctgg aaccttgatt attacagaga caaatgttcc tcatgaagaa aatatctctt
     2281 acttcggaaa tggagaagat gaagcccata tggtttataa ctttgcactt ccccccttag
     2341 tactacatac catgtataca atggacagta ccgcactttc aaaatgggct tcaacacttg
     2401 acacctgctc aaataacaca acatatttca attttcttga ttctcatgat ggaataggac
     2461 tcatggctgt taaggatatc ttaagtccaa atgatattga tttaatactt gataacgtaa
     2521 gacaacatgg gggatatatc agttataaaa ctggagaaaa cgagaaggaa attccttatg
     2581 aaatgaatat aacctggttt agtgcgatta ataatactaa taaccccgag aataacgaca
     2641 ttgcatatca ggtaaaaaaa tttgttgcat caagatcaat tgcccttgta ttgcagggta
     2701 ttcctggtat ttatttgcac agtctaatag gaactgtaaa cgatattgat gctgttgttt
     2761 cttcaaaagc aaatagagat atcaatcgca caattattga cgaacaggaa atctcttatg
     2821 ctttaggaga cggactttct aagatatccc gcattaaccg tgaatacggt cggataattg
     2881 acatccggac aaaaagtaga gcatttcatc caaacagcaa acaaaaggtt gtttttaaat
     2941 ctgaatccct tttttctgtt atacgtttta cagagactaa agacaatatt atttttacta
     3001 tagtaaatat cagtaacgag acggttcata cattattaac aaataatgaa cttgaagaaa
     3061 tttctaaaaa ctggattgac cttattagcg gaatgcctta tagctgtgat gatgcggggc
     3121 tacatcttgt attgcagccc tacgacattt tatggctctc tcctgaaaaa ctataaaact
     3181 atacttgata aattgattga aaaaaatcag ctatgttttg cttattgtta tgtaaaaaat
     3241 cttctagttt ttttatatct gaatttttta tagctatatt ttcaagatcg gtttcactaa
     3301 tacgaggatc cggtatatcg tcaattgact cataaaaaaa ttcctggcat aacgcatcgc
     3361 atatacgcaa tattgacatg tcttcagagc taacgtttga aggtatatcc tgtgaatggt
     3421 gatacataac cggatcagat atctcgtctg gaaagttcca ccttttcaat agcagggcgc
     3481 ccgcttctcc atgatcgaca cctatgattt ttttttctga tttagcgagg gaatatgttc
     3541 cagaattctg cacatccaat acagcttgtt caaaaagatc actaaaaaaa atatcgaaaa
     3601 caagcttacc aatatcatga agaagagcag ctacaaacag ataatctttc ttttttgtct
     3661 taataatatt caaacacaat tctcgagtta aagtcgcgac agccacgcta tgaaaccaaa
     3721 gccccgtctc agaaaagaac ctttttgatc taggttcaga aagagtatta acaacgccaa
     3781 gactaaccgc caaagatttc accatattaa acccaagcaa agacacagca cgatctattt
     3841 cgctaatttt ttttggaaat ccataatatg acgaattggc aacttttaat attttcgaaa
     3901 caagagcggg gtcgtatgaa ataacctcgc taattttttt tacgtctgat ttattatctt
     3961 ccatcatctg aagtatttta ggtataacaa caggtaatgt aggcaactcg tttactcttg
     4021 aatacagtat tttttttaga tcgggtaaat ccagagccat aattcctcca gggctatttc
     4081 atggtattta aaaattcatc aagactcaat gtgccactca taagctttct tgccattaac
     4141 gctgccgagc tatcaccaat aatcatggta gcaagagctt ttccattttt ataatatatt
     4201 tttttataca cagaatcatt tattactacc tttttttcag cattcgcaaa ctcctccgat
     4261 aatggatcat atccggctga aaaaagatca atacctgtta tcttcaaaac tacaggcgga
     4321 taagatccct tataggtctc gctcattcca caagctgata aaccagcagt tttaccctgt
     4381 tccttggcac ttatccaaag cccgaacaat tttccctcca tttgtataca atcaccagca
     4441 gcgtaaattc ctttaaccga tgtttcaaga tgattattaa cgacaattcc tttgtcacat
     4501 tttattccgc tagacagagc aagatcaatc tcaggtctaa tacccgccga aaacacaagc
     4561 atatcagctt caattttttc acctgtagac aaagtcaatg attcaatttt ttcattcccg
     4621 tttatctgtt taacagatgt tccacagata aattttagac cttttttaat caaaagttgt
     4681 tttaaaaatc cccccccctg atcatcaagc tgtcttggta ataaattatc tgctatctca
     4741 acaactgtta catctttaca aacacgcaac agagaattag ctgtttcgag ccccaagagc
     4801 ccgcctccag ctataacaat tttttttaca gactctgcat agctattaat caaatcggca
     4861 tctccgaaat ttcttaaaga aaaaacacct ttaagattcg agccttctac cggaggtaaa
     4921 aaatttctag ctcccgtagc tattatcaaa aaatcatact tatgttcttt tcctttacta
     4981 tctagaatta ttttcttatc attttctatt gaaacaatct tgcaatttaa attaacttta
     5041 atttttttct gtatatataa atcttcatta tgcaatatta gatcttctgg cttcatttgt
     5101 gatccaacat attcaacaag ttttggacga tagtaaagat gatggtttga agaggtaaaa
     5161 atttcaatat ctgcttcttc gtttttcgtc cgaattccca aagcagcgtc agttcctgcc
     5221 gcaccgttac caattataca atatttcatt tttttacctt ttctttatta ctattctttc
     5281 ataatgaatt atacttgtaa aaaaaacagg gctttgatat tgatgaaatt tatacctgct
     5341 tgtctctata ctccactttt atagcgggta caatttttat caaacgattt ttggaggcac
     5401 aatggaaaag tatgtttgta cagtttgtgg ttacgagtac gatccggaaa taggtgatcc
     5461 ggacagcggc ataaagccgg gtacatcatt tgaagatata ccggatgatt gggtctgtcc
     5521 cgtatgcggt gtaggtaaag atcaatttga accagcttaa taaataacaa ataccggttt
     5581 tcaaatatta ttttgataac cggtattttc actctattcc acacttttaa tattaacaac
     5641 atctacacta taaggattac agctcttgcc tgaatatttt acaatt
//
LOCUS       DAOTGZ010000182         5646 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_3064
            NODE_16971_length_5646_cov_5.427115, whole genome shotgun sequence.
ACCESSION   DAOTGZ010000182 DAOTGZ010000000
VERSION     DAOTGZ010000182.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868088
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 5646)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5646)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:22:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,785
            CDSs (total)                      :: 2,751
            Genes (coding)                    :: 2,732
            CDSs (with protein)               :: 2,732
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 12 of 19
            Pseudo Genes (internal stop)      :: 5 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5646
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16971_length_5646_cov_5.427115"
                     /isolate="AMR_MDS_3064"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            153..722
                     /locus_tag="P5123_10750"
     CDS             153..722
                     /locus_tag="P5123_10750"
                     /inference="COORDINATES: protein motif:HMM:NF012894.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015288 - porin activity [Evidence IEA];
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HRX16782.1"
                     /translation="MLPSTYVYALTGITEVHNPEYNDQKVLLSYQRKINVVQGKGAGD
                     TEEISDADMDRIRKILNSIHFEEVLLISDKILFESGSSKILESSYEELDKIVAVLNEK
                     KVSVRIDGHTDNIGSDSKNQQLSLDRAESIRTYLVEKGIDPERLSAAGFGASQPVTDN
                     STKAGRAQNRRVEFIVVEQNSSKGLFWWL"
     gene            738..2279
                     /gene="zwf"
                     /locus_tag="P5123_10755"
     CDS             738..2279
                     /gene="zwf"
                     /locus_tag="P5123_10755"
                     /inference="COORDINATES: protein motif:HMM:TIGR00871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004345 - glucose-6-phosphate
                     dehydrogenase activity [Evidence IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate dehydrogenase"
                     /protein_id="HRX16783.1"
                     /translation="MSDNISATIDIPKSRFIKPCGMTSDNAPLDPVTIVIFGGGGDLS
                     RRKLIPTIYHLFKENLLPDDFSVIGFGSPPDGDDVSYRERIVLSIKEFEPGLYEKEGF
                     DTFLNHVFFEGGYFDDISKYENLKKRLNSSPGHNVIYYMSVHPSFLPQIINNIKSCSL
                     NDTNYNPRIVVEKPFGTDQKSALELNSLLHSCFEENRIFRIDHYLGKETVQNIIYLRF
                     ANIIFEPLWNRNNIDSVQITVAEDIGVEHRGKFYEQAGVVRDIVQNHILQLMAFIAME
                     PPVGFDADAIRDEKVKVFNSIRMMDDEYVSKNCVQGQYGSGMIGGQKVSGYRDEFRVA
                     SDSKTPTFFAAKMFIDNWRWADVPFYVRTGKRLPKRCTEVIICFKRPPLKIFGTACDD
                     IEQSLLRIRIQPNEQISFFTNVKYPNDAYRMSRVSMNFDYKDYFKEKNYPPYSRLILD
                     CLKGDLTLFVREDSIKALWSIVDPIINKLESSEYTCPNYSAGDWGPVEADNLLKRDGR
                     SWYTD"
     gene            2291..3001
                     /gene="pgl"
                     /locus_tag="P5123_10760"
     CDS             2291..3001
                     /gene="pgl"
                     /locus_tag="P5123_10760"
                     /EC_number="3.1.1.31"
                     /inference="COORDINATES: protein motif:HMM:TIGR01198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0017057 - 6-phosphogluconolactonase
                     activity [Evidence IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6-phosphogluconolactonase"
                     /protein_id="HRX16784.1"
                     /translation="MNEIVTNELPTLYELLLKKIENLYEQFVASKGFFTIALSGGSTP
                     LPLYSLLAKTNAIDFNKVFFFIVDERFVDLNSNDNNYNAINKALFSKIDNNSLNILYI
                     DPTFDTPDESAQDYENKIKNFFSEKGTAPAMDVVLLGIGDDGHTASIFPGDSTLFLKN
                     SICTATDLVYNNYFRITLTADFINKSEYVFFLVIGENKKNAVFKVVQKKAKNLPASHI
                     NAKVVNYFLDPLAASCLK"
     gene            complement(3061..3255)
                     /locus_tag="P5123_10765"
     CDS             complement(3061..3255)
                     /locus_tag="P5123_10765"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRX16785.1"
                     /translation="MKFIIMNLLSIFVGVYFFVMGIKNIRNEENELRGVIMALGIIFL
                     PVGALLTIAGIVNFYFMIFK"
     gene            3566..4285
                     /locus_tag="P5123_10770"
     CDS             3566..4285
                     /locus_tag="P5123_10770"
                     /inference="COORDINATES: protein motif:HMM:NF013760.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_process: GO:0015031 - protein transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MotA/TolQ/ExbB proton channel family protein"
                     /protein_id="HRX16786.1"
                     /translation="MKLKKVIFTISAIFASSGMIFAEAVNGEKVFSLLELYKQGGLFM
                     WPILILSAVGLGFVLERIVFFIISKLGSKEFIDKLEHTINSQGLEEVEKLCSQNRNKV
                     SLIIQKGLALKSLGYERVEKTLSVAASVEVAMLERGLNVIAAIANIVPMLGFLGTVTG
                     MITAFNDIASADQVSAKIVAGGIKEALLTTSFGLIAAIPLLLLYNYFVHRTEEFVTQI
                     ERLSSDIVEKIIKGNVVELND"
     gene            4297..4707
                     /locus_tag="P5123_10775"
                     /pseudo
     CDS             4297..4707
                     /locus_tag="P5123_10775"
                     /inference="COORDINATES: protein motif:HMM:NF014525.2"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="biopolymer transporter ExbD"
     gene            4707..5126
                     /locus_tag="P5123_10780"
     CDS             4707..5126
                     /locus_tag="P5123_10780"
                     /inference="COORDINATES: protein motif:HMM:NF014525.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biopolymer transporter ExbD"
                     /protein_id="HRX16787.1"
                     /translation="MIVSRKNKKKSVLPVSAMSDIAFLLLIFFMLSSISEEDKEIQIE
                     LPSSRVSVQEDKKFFNVWVDSKGDIFFNAKKGTPTALTTYALYKLKADPETRVLIRAS
                     RDVSYSVIDSVFESLREAGAYNVVLVSEKRYYKDPNI"
     gene            5148..>5646
                     /locus_tag="P5123_10785"
     CDS             5148..>5646
                     /locus_tag="P5123_10785"
                     /inference="COORDINATES: protein motif:HMM:NF015506.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="energy transducer TonB"
                     /protein_id="HRX16788.1"
                     /translation="MSDSISVSGNYKNMPVFINYFRLRPEFVFSLAWFMLLVCLVYTV
                     AITPSSVDKSEFMDLGQFEMVELTIPVPEAAQPEIKINDKSEEEVKTKAANNMKFGND
                     SGKWDPSAMAATMPKPKTALPVFPDALKSQGVEGVVILEVGIDENGTVVYGKIVKSVH
                     ALLDKL"
BASE COUNT         1844 a          863 c         1161 g         1778 t
ORIGIN      
        1 catagggtga tttaggtttt gaaataggaa aatcacccta tgttttcgaa gaaaatggtg
       61 aagaaactac tgacaatgcg tcagaagaga ctaagaagga gtccggcaag agtgatgaag
      121 agcgtcatga aaaagtaact gatgattcaa ctttgttgcc ttcaacttat gtttacgcgc
      181 ttactggtat taccgaggtt cataatcctg aatataatga tcagaaagta ctactctctt
      241 atcaacgcaa gataaatgtc gttcaaggaa agggtgcggg tgatacagaa gaaatttcgg
      301 atgcagatat ggatcggatt cgcaagattc ttaactctat acacttcgaa gaggtgctat
      361 tgatatctga caagattctt tttgagagtg gaagttcaaa aatacttgag agctcttatg
      421 aagaattaga caagatagtt gctgttttaa atgaaaagaa agtgagtgtt cgtatagatg
      481 gacatactga taatataggt tcagattcaa agaatcagca actttcactt gatcgtgccg
      541 aatcaatacg aacttatctg gtagaaaagg gtatcgatcc tgaacgatta tcagctgccg
      601 gttttggtgc gtcacaacct gttactgata actctacaaa agcaggcaga gcgcaaaata
      661 gaagggtcga gtttattgtt gtagaacaaa acagcagcaa gggtttgttc tggtggttgt
      721 aaaaatcggg ggatttggtg agtgacaata tttctgcgac aatagatata cctaaaagca
      781 ggtttattaa accatgtggt atgacttcgg ataatgctcc gctggatccg gtaactattg
      841 taattttcgg tggaggcggt gatctttcac ggcgtaaact tatacctact atttatcatc
      901 ttttcaaaga aaatctcttg cccgatgatt tttctgttat tggtttcggg tcacctccgg
      961 atggtgatga cgtgtcttat cgcgaaagga ttgttctttc gatcaaggag ttcgaacccg
     1021 gcctttatga aaaagagggg tttgatactt tcttgaatca tgtttttttt gagggcggat
     1081 attttgatga tatttctaaa tatgaaaatt tgaaaaaaag actgaacagt tctcctggac
     1141 ataatgtgat ctattacatg tcggttcacc cctctttctt accccaaata attaataata
     1201 ttaaatcatg ttctcttaat gatacgaatt ataatccacg cattgttgta gaaaaacctt
     1261 tcggtactga ccaaaaatca gctcttgagc ttaacagcct gttacatagt tgctttgaag
     1321 agaaccgcat atttagaatc gatcattatc ttggcaagga gaccgttcaa aacataatat
     1381 atttgcggtt tgcaaatatc atttttgaac ctttgtggaa cagaaataat atcgattctg
     1441 ttcaaataac ggtggcagag gatattgggg tggagcaccg aggcaagttt tacgagcagg
     1501 caggggtggt aagggatatt gtacaaaacc atattttaca gttaatggcg tttattgcta
     1561 tggagcctcc tgtcggtttt gatgctgatg caattaggga tgagaaggta aaggtattta
     1621 actcaataag aatgatggac gacgagtatg tatcgaaaaa ttgtgttcag ggacaatatg
     1681 gatccggtat gattggcggc caaaaagtct ctggatacag agatgagttt agagtagcct
     1741 cagattctaa aactccaaca tttttcgctg caaaaatgtt tattgataac tggcgatggg
     1801 cagatgttcc tttctatgtt agaaccggaa agcgattgcc caaaagatgt accgaggtta
     1861 ttatctgttt caaaagacca cccttgaaaa tatttggaac tgcttgtgat gatattgaac
     1921 agtccttgtt acgaattaga attcagccaa acgaacagat atcttttttt acaaatgtga
     1981 agtatcccaa tgatgcctat cgcatgagcc gggtatcaat gaatttcgat tataaggatt
     2041 attttaaaga aaaaaattat cccccttact caaggttgat cctcgactgt ctcaagggtg
     2101 acctgaccct ttttgtgcga gaagatagca taaaagcgtt gtggagtatt gttgatccga
     2161 taatcaacaa actggaatca tcagaatata cttgtcctaa ttatagtgcg ggcgattggg
     2221 ggcctgttga ggcagataat ctgttaaagc gtgacggccg tagttggtac acggattaaa
     2281 gaggcgggtt atgaatgaga ttgtaacaaa tgagttgccc accttatatg agcttctttt
     2341 aaaaaaaata gaaaatcttt atgaacagtt cgtagcatcg aaaggttttt ttacaattgc
     2401 tctctcgggt ggatcaactc cgcttcctct gtattcactt ttggccaaga cgaatgccat
     2461 tgattttaat aaggtttttt tcttcattgt tgatgagaga ttcgtagatt tgaattctaa
     2521 cgataataac tataatgcaa ttaataaagc actattttcc aaaattgaca ataatagtct
     2581 aaacattctt tatatagatc ccacattcga tacacccgat gaaagtgccc aggactatga
     2641 gaataaaatc aaaaattttt ttagtgaaaa aggtacagca cccgctatgg atgttgtttt
     2701 gcttggtatt ggtgatgatg gacacactgc atcaattttt ccgggcgata gcacactttt
     2761 tttaaagaac agtatttgta cagcaacaga tttggtttat aacaattatt ttagaattac
     2821 attaactgcc gattttatta ataaaagtga gtatgtcttc tttttggtta ttggagagaa
     2881 caaaaaaaat gctgtattca aggttgtaca aaaaaaagca aagaatcttc ccgcgtcaca
     2941 tataaatgct aaagtggtaa actattttct ggatcctctt gcggcatcct gtcttaagta
     3001 ggtaggtatt tatttgaaaa tattgttcgg tgattaactg attagtttat gctgcctagt
     3061 ttatttaaag atcataaaat aaaaattaac aattccagca atagtaagta atgctccaac
     3121 aggaagaaaa attattccaa gagccataat aacgccccgt aattcgttct cctcgtttct
     3181 aatatttttt attcccatta caaaaaaata cacaccaaca aaaatggaaa gcagattcat
     3241 tataataaat ttcatatgat cctcgtttat agattaaaat agctatttca caatatttaa
     3301 taaatcttga gataataaac tgataattaa ccttgttttt gtcaagaagg aagttagtta
     3361 acaattattg aattatcttg caattaatac cttttttaat gtgaatgcgt gattgagatt
     3421 aaaatgcaga ttgcaataat tttgagtaat taacaaaaaa ttaataattt tattaataaa
     3481 tccatacttc gactgtataa atcataataa cagttagcaa ttgtttgcag actgaatatc
     3541 aaaaggaaaa tggatttaac aagaaatgaa actaaaaaaa gtaatattca ctatatcggc
     3601 tatttttgcg tcaagtggaa tgatttttgc ggaagctgta aatggagaaa aagttttttc
     3661 tttgctggaa ctttataagc aaggtggtct tttcatgtgg ccaatactga ttcttagtgc
     3721 ggtggggctt gggttcgtgt tagagcgcat agtattcttt attataagca agttgggctc
     3781 taaggagttt attgacaaac tggaacatac aataaatagc caagggcttg aagaggttga
     3841 aaaactctgt tctcagaacc ggaataaagt ttccttgatt atacagaaag gtttggcatt
     3901 aaagtcactt ggctatgaaa gagtagaaaa aacgttatcg gttgccgctt ctgtggaagt
     3961 tgccatgctg gaaagaggct taaatgttat tgctgcaata gcaaatattg ttccgatgtt
     4021 aggttttcta ggaactgtta ctggtatgat taccgctttc aatgatattg catcggctga
     4081 tcaggttagt gcgaagatag ttgcgggcgg aatcaaagag gctctattga ctacttcgtt
     4141 tggattgatt gctgcaattc ctttactttt gctttataac tattttgtac atcgaacaga
     4201 agaatttgtg acacaaatag agcgtctttc atcggatatt gtagaaaaaa ttattaaagg
     4261 caatgttgtt gaattaaacg actaacgggg ttaacggtga aagttagacg gaatagtaga
     4321 ggtacgagtg agataaatct ttcttcgtca tcggatattg catttttatt gataatattc
     4381 tttatggtga cttcggcctt cattttcaag gatggtttaa aactggttct ttctgctaag
     4441 agtaaagaaa ctaagattat agaaaatctt gaagatattt caattgtatc agttacaaac
     4501 gataaactta ctttgcaata aaaagatatc acaccattaa atcttgaagc agaattaaaa
     4561 gagattattc ataaagattc agagtcggta gtactgctcc aaattgaaaa agatacccgt
     4621 tacgaaagag taatcgaaat agtggatatt atgaaagttg cggatgtaaa aagattatca
     4681 atgaaaataa acaaagagaa agaataatga ttgtgtcacg caaaaataaa aaaaaatcgg
     4741 ttcttccggt ttcggctatg tcagacattg cctttttact tcttattttc tttatgcttt
     4801 cgagtattag tgaagaagac aaagaaattc agatagagct gccaagttca cgagtatcag
     4861 ttcaagaaga caaaaagttc ttcaatgttt gggtagattc aaaaggcgat atatttttta
     4921 acgctaagaa aggaactccg acagctctta caacatacgc tctttacaag ttaaaagctg
     4981 atccggaaac aagggtactt ataagagcgt ctcgtgatgt ttcatattcg gtaatcgatt
     5041 ctgtatttga atcgttaaga gaggccggag cgtataatgt cgttttagtc agcgagaaac
     5101 gttattataa ggatccaaat atttaattat atttttttgg aggctgtatg agtgattcaa
     5161 tttcagtgag tggaaactac aaaaatatgc cggtatttat aaactatttc aggcttaggc
     5221 cagagttcgt tttttctcta gcatggttta tgttacttgt ctgtcttgta tatactgttg
     5281 ctattacgcc atcatctgtt gacaagtccg aatttatgga tcttggacaa tttgagatgg
     5341 ttgaactaac gattccggtt cccgaggctg ctcaaccaga aataaagatt aatgataaaa
     5401 gtgaagaaga ggttaagaca aaggctgcca ataatatgaa atttggtaat gattcaggaa
     5461 aatgggaccc ctctgcaatg gccgctacaa tgccaaagcc caagaccgcg ttacctgtct
     5521 ttcctgatgc cttaaaaagc cagggagtgg aaggagtagt aattcttgaa gttggcatag
     5581 atgaaaatgg aacagttgta tatggaaaaa tagtaaaaag tgtacatgct cttttagaca
     5641 agcttg
//
LOCUS       DAOTGZ010000183         5571 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_3064
            NODE_17292_length_5571_cov_5.468274, whole genome shotgun sequence.
ACCESSION   DAOTGZ010000183 DAOTGZ010000000
VERSION     DAOTGZ010000183.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868088
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 5571)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5571)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:22:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,785
            CDSs (total)                      :: 2,751
            Genes (coding)                    :: 2,732
            CDSs (with protein)               :: 2,732
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 12 of 19
            Pseudo Genes (internal stop)      :: 5 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5571
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_17292_length_5571_cov_5.468274"
                     /isolate="AMR_MDS_3064"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..533)
                     /locus_tag="P5123_10790"
     CDS             complement(<1..533)
                     /locus_tag="P5123_10790"
                     /inference="COORDINATES: protein motif:HMM:NF038196.1"
                     /note="N-terminal region resembles flavodoxins. C-terminal
                     ferrodoxin region binds two 4Fe-4S clusters; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EFR1 family ferrodoxin"
                     /protein_id="HRX16789.1"
                     /translation="MTIFFFTASGNSLKVSKAFITSDCVSISSELRKSPGNYTDDKIG
                     FVFPSYFYGIPRPVLKFIRRSDFNSSYFFTIITCGSTSGATSLQLQNELKKKGISLNY
                     AVEITTINNYVPMFDINKEIYTQSKKNIESILPAAVHDIRASVNRIPSFGILAKFSHT
                     IAQIMYNRMLDKCDKNL"
     gene            729..1766
                     /gene="rlmN"
                     /locus_tag="P5123_10795"
     CDS             729..1766
                     /gene="rlmN"
                     /locus_tag="P5123_10795"
                     /EC_number="2.1.1.192"
                     /inference="COORDINATES: protein motif:HMM:TIGR00048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_function: GO:0016433 - rRNA (adenine) methyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0031167 - rRNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(2503)-C(2))-methyltransferase
                     RlmN"
                     /protein_id="HRX16790.1"
                     /translation="MNNFGSSFIKNFTISEAEKLFRDLDEKPYRATQLFAWLYGKNCD
                     SFDDMSNFSKDLRNYLNQNFIVNPLVCEQKLVSEIDGTEKYLFKTVDDHYIESVLIKN
                     EESDSGRLTICISSQIGCQMGCTFCSTAKIPFIRNLEAAEIIDQICQVRRISGLNNNN
                     IVFMGMGEPFMNYDNVIKAANIMNYSFGLHISVRKITISTCGILPRINQFIDEGHLFN
                     LAVSLNDTDTTKRSETMPVNKKWPIEDIAALFNKKMPKAHNRLTLEYVMRKDNISRAD
                     ALRLKKMFKYSRIKLNLIPLNGEGTPSENDISSFIKELEILNIPITVRKSLGRDINGA
                     CGQLSGQKYRK"
     gene            1776..4076
                     /locus_tag="P5123_10800"
     CDS             1776..4076
                     /locus_tag="P5123_10800"
                     /inference="COORDINATES: protein motif:HMM:TIGR02074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PBP1A family penicillin-binding protein"
                     /protein_id="HRX16791.1"
                     /translation="MDLLKRIYGWIISHKLIVFVITPGAFSLLILFYVFIIYLSWLSD
                     REAALDKLARYKRLIDRTEEMSEGMIYAAGDYGLEEKVVDLPTSIVDRKGEIIGQFFS
                     QKREIVPYDHIPDPLIKSVIASEDRDFYDHNGVNPKGILRAFLINIKNFRVVQGGSTI
                     TQQLGKVLFTDMERNLKRKIYEVFCAQEIEKYYDKQDILSMYLNLIYFGNGAYGVEAA
                     SKMFFGNSVSTLNETECAMIVATISSPRSYSPLSNLPNSIKKTRRILQSMIDAGFMTE
                     KRAEIQYKKFLRKWDVVFDENDVPTSSLVGSFVFSSYRINRAPFLVEQIRRLLVEKFG
                     EDVLKKGGLTVHTTVDAEYQDYALNALRSGVERQREYHRALAAKLGKKKSGLQHSENA
                     KNIQGALVAMDPLSGEILAYVGGHEFTSGNQLDHVSQIRRQPGSSIKPLLYCSAIENR
                     DITPSTIFIDEATTFEGGYSPKNYGGKYYGPIIVLEALKRSVNIIAVKILEKTGYDTV
                     FGYIKKALHMDQSEFNNRFQKTLSFALGTYELSPLENAVLHSTIVNGGQFVKPYTIKT
                     ITDYSGRVIWNNEEEIVRFTKEKRKEYGNIIDPIAAAITVSLAKNVFKPGGTAAGTAA
                     RYKIDFPAGGKTGTSTNYNDAWFVGYTTKFVTAVWIGNSKGAITLGAGRSGGSLAAPV
                     WGEFVSKTFAFDKPDDFPMPQEGVSFQTIDLKTGKVPRVVTDPSTVSMDQIFYEGTEP
                     GVYVDEFTMPGDEDTSEESSIVDGEE"
     gene            4079..4930
                     /locus_tag="P5123_10805"
     CDS             4079..4930
                     /locus_tag="P5123_10805"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013700.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M23 family metallopeptidase"
                     /protein_id="HRX16792.1"
                     /translation="MGIRSLAVCIVILIIINTGFKWPQSEFRITSTFGESRTDHFHDG
                     VDIISSSRSFYPVADGIVLYVWNRAFYPFENYTGSGNYAIIDHGEHASAYLHLEDSPI
                     LKQRVSSNEPLCLYQNTGRSYGSHLHFGIYNRASWNSYNFLKLVDPLEDNIAPVIDHI
                     GFSIDGSIIRVSDNSQIRLTAHHPVLFNMFDSIQNGDRHGIYKLIVYLNGKEIRNITF
                     DELVYGKNGLTISGMMFQDIFEGKDYYKVNGIIYNNGVNKFKVVATDLAGNSTEKEFT
                     LTIRLDF"
     gene            4951..5367
                     /locus_tag="P5123_10810"
     CDS             4951..5367
                     /locus_tag="P5123_10810"
                     /inference="COORDINATES: protein motif:HMM:NF024252.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HRX16793.1"
                     /translation="MIPIIRRDKKISQARLAKMIGVSPSYLCKIEKSLQQPSVDFVKK
                     CSEILDVPENELFPDKINSKEIIKIMSQKNNPLWVERRKLGMKQVELARLVKCSPSYL
                     SKVENNQLVPTDFFQSQCSKVLKKKRSYLFPFIQKK"
     gene            5364..>5571
                     /locus_tag="P5123_10815"
     CDS             5364..>5571
                     /locus_tag="P5123_10815"
                     /inference="COORDINATES: protein motif:HMM:NF013741.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CNNM domain-containing protein"
                     /protein_id="HRX16794.1"
                     /translation="MIIIDPIFLIIFLFLLLSAFFSGSETALFSITKSELYNLAFSEK
                     RSDNLIASIMKKPRKALITILIGNL"
BASE COUNT         1782 a          894 c         1114 g         1781 t
ORIGIN      
        1 tatagattct tgtcgcattt atcaagcatt cgattataca ttatttgggc aattgtatga
       61 gagaatttag caagaatgcc aaaagagggg atacggttaa ctgaggctct tatatcgtga
      121 accgctgctg gcagtatcga ttcaatattc ttttttgatt gagtgtatat ttctttatta
      181 atatcaaaca tgggaacgta gttgttaatg gttgttattt caactgcata attaagagag
      241 attccttttt tcttaagctc attttgcaat tgtaaagaag ttgctccgct tgtcgaacca
      301 catgttataa ttgtaaagaa gtaagagctg ttaaaatcag atctacggat gaattttaaa
      361 actggccttg gaatcccgta aaaatatgaa gggaatacaa agccgatttt gtcgtcagtg
      421 tagtttccag gactttttcg aagctcgctc gatatggata cgcaatctga tgttatgaat
      481 gcttttgaaa cttttaaact gtttccggaa gccgtaaaga aaaaaatggt catagataaa
      541 ctctcaatag catttatttg atcggttgaa accctataaa gtcgtttgat tatgcgtcaa
      601 ttaatatttg gtaataatct ttttttattg gtacataatg gttgtaatct gttgattttt
      661 attaaaactt gcctggctta ttgactattc ttgcttgacg gtagagctct ctttaatagg
      721 tattttatat gaataatttt ggcagttcgt ttatcaaaaa ttttacaatt tccgaagcag
      781 agaaactctt tagagatttg gatgaaaaac catatagggc aacacagcta ttcgcatggt
      841 tatatggtaa aaattgtgat tcattcgatg acatgagtaa tttttctaag gatttacgta
      901 actatttaaa tcagaatttt attgtaaacc cgcttgtgtg tgaacaaaag cttgtttctg
      961 aaatagacgg taccgaaaag tatcttttta aaacagtaga tgatcactat atagaatccg
     1021 ttctaataaa aaatgaagag tcagattcag gacgcttaac tatatgtata tcttcccaaa
     1081 ttggatgtca aatggggtgt actttttgca gtacagcgaa gattccgttt atacgtaatc
     1141 tcgaggctgc ggaaattata gatcaaatat gtcaagttcg aagaatatca ggtttgaata
     1201 ataataatat tgtctttatg gggatgggtg aaccatttat gaattatgat aatgttatta
     1261 aagccgccaa tattatgaac tacagtttcg gattgcatat ttcagtaaga aaaattacta
     1321 tatccacttg cggtatattg cctcgaatta atcagttcat tgacgaaggc catctgttta
     1381 acctggcagt ttcacttaat gataccgata ccactaaaag atctgaaaca atgccggtta
     1441 ataaaaagtg gcctatagaa gatattgctg ctctgtttaa caaaaaaatg cctaaggctc
     1501 ataacagatt aactcttgaa tacgtaatgc gaaaagataa tatttctaga gctgatgctt
     1561 taaggttgaa aaagatgttc aagtattcac ggataaagtt gaatctgata ccccttaacg
     1621 gtgaaggtac tccttcagag aatgatattt cctcttttat aaaagagctt gaaatattaa
     1681 acataccgat tacagttcgt aagagccttg gtcgtgatat caacggtgct tgcggacaac
     1741 tttccggtca aaaatacaga aaataaagga ttattatgga tttacttaaa agaatatatg
     1801 gatggataat ttcacacaaa ctgatagttt ttgtaattac tccgggagcc ttttcgttac
     1861 tgattctgtt ttatgtattt atcatatacc ttagctggct ttctgacaga gaggctgctt
     1921 tggacaagct ggctcgttat aaacgtctta ttgaccggac tgaagagatg agtgagggaa
     1981 tgatctatgc tgccggtgat tatggattgg aagaaaaagt agttgatttg ccaactagca
     2041 ttgtagatcg gaaaggcgaa attatcggtc aatttttcag tcagaaaagg gaaatcgtcc
     2101 cttatgacca tatcccagat ccattaatca aatctgtaat agcctctgaa gatcgtgatt
     2161 tttatgatca taatggagtt aatcccaagg gtatattgcg ggcttttctg attaatatta
     2221 aaaatttcag agtggtgcag ggggggagca ctattaccca acagttaggg aaggttctgt
     2281 ttactgatat ggaacgtaac cttaaacgta aaatttatga agtattttgt gctcaagaaa
     2341 tagagaagta ttatgacaaa caggatatac tctccatgta tctcaactta atctattttg
     2401 gtaacggtgc ctatggtgtt gaagcggcat ccaaaatgtt tttcggtaac tcggtttcaa
     2461 cattgaatga gaccgagtgc gcgatgattg tggcaacaat atcaagccca cgttcatatt
     2521 cacccctctc gaatttgcct aattcaataa aaaaaacaag acgcatttta cagtcaatga
     2581 ttgacgccgg ttttatgaca gagaagaggg cagagataca atataaaaag tttttacgaa
     2641 aatgggatgt tgtatttgat gaaaacgatg tacccacctc ttcattggta ggaagttttg
     2701 tttttagctc gtaccgaatt aatcgtgcac cgtttcttgt agagcagatc agacgattgc
     2761 ttgtagaaaa attcggtgaa gatgttttaa agaaaggtgg tttaacagtc catactacag
     2821 ttgatgcaga gtatcaggat tacgcattaa atgcgctgag aagtggagtt gaaagacaga
     2881 gagaatatca tagggctttg gccgcgaaac ttggtaaaaa aaagtcaggt ttgcaacatt
     2941 ccgagaatgc gaaaaacatt cagggagcgc tcgttgctat ggatccgctt tcaggcgaaa
     3001 ttctggctta tgtaggagga catgaattta cctcaggtaa tcagttagat catgtctctc
     3061 agatacgcag acaaccaggt tcttctatca agcccttgct ttactgttca gcaatagaaa
     3121 atcgtgatat tacaccttct acaattttta ttgatgaagc aacaaccttt gaaggcggtt
     3181 attcgccaaa aaattatggg ggcaaatatt acggacctat tattgtttta gaggcgttaa
     3241 aaaggtcggt aaacattatt gccgttaaaa tcctggaaaa aacaggttat gatacagtct
     3301 ttggttatat aaaaaaagct cttcatatgg atcagagtga atttaataac cgttttcaaa
     3361 aaacactctc atttgctctg ggaacatacg aactatcacc tttagaaaac gcggtattgc
     3421 actctacaat tgtcaatggc ggtcagtttg ttaaacccta tacaattaaa accatcacag
     3481 actatagtgg gcgcgttata tggaataacg aagaagaaat tgtgagattt actaaagaaa
     3541 aacgaaagga atatggaaac attattgacc cgattgctgc cgcaattacc gtttcgcttg
     3601 ctaaaaatgt ctttaaaccg ggtggtacgg cagcgggtac agcggcaaga tataaaatag
     3661 attttccggc tggaggtaaa acaggtactt cgacaaatta taatgatgcc tggtttgttg
     3721 gttatacgac aaaatttgtt accgcagtct ggattggaaa ctcaaaagga gccattactc
     3781 tgggtgctgg aagaagcggt ggttcacttg ctgcgcctgt atggggagaa tttgtttcaa
     3841 aaacatttgc ttttgataaa cccgatgact ttccaatgcc gcaagaaggt gtttcttttc
     3901 aaacaattga tctgaaaact ggaaaagttc cccgagtcgt aactgatcct tcaactgttt
     3961 caatggatca aattttttac gagggaacag aacctggcgt atatgtagat gagtttacta
     4021 tgcctggtga tgaagatact tctgaagaat cgtctatcgt agacggggag gaatagttat
     4081 gggtattcgc tctttagcag tttgtattgt gattttgata ataataaata ccggttttaa
     4141 atggcctcag tcagagttta gaattacctc aaccttcgga gagtctcgca ctgatcattt
     4201 tcatgatggt gttgacatta tatcgtcatc acgttctttt tatcctgtgg cagacggtat
     4261 tgtgctctat gtgtggaaca gggcgtttta tccttttgaa aattacacag gaagcggtaa
     4321 ctacgctata atagatcatg gtgagcatgc gtcagcgtac cttcatcttg aagattcacc
     4381 tattttaaaa caacgggtaa gcagtaatga accgttatgt ttgtatcaga ataccggtcg
     4441 atcttacgga agtcatctgc attttggaat atataatcgt gcaagctgga attcttataa
     4501 ttttttaaaa cttgttgatc cgcttgaaga taatattgcg cctgtaattg atcatatcgg
     4561 attctctatt gatggaagca taataagggt ttcggataat tcacagattc gtcttactgc
     4621 acatcatccg gttcttttta atatgttcga ttcaatacaa aacggagaca gacacggtat
     4681 atataaatta atagtttatc tcaatggaaa agaaattcga aatattactt ttgacgagct
     4741 tgtttatggc aaaaatgggt tgactatttc ggggatgatg tttcaggata tatttgaagg
     4801 taaagactat tataaggtca acggcattat ttacaataat ggtgtcaaca aatttaaggt
     4861 cgtagctact gatttagctg gcaattcgac tgaaaaagaa ttcacattga ctatcagact
     4921 cgatttttaa ggaagtaatt agtgaataac atgattccga ttatcagaag ggataagaaa
     4981 atatctcagg cacgtcttgc taaaatgatc ggcgtatcgc cttcttacct atgcaaaatt
     5041 gaaaaatctc ttcaacagcc atctgtagat tttgtcaaaa aatgttctga aatacttgat
     5101 gtgccagaga atgaactttt tcccgataaa atcaattcta aagaaataat aaaaataatg
     5161 tctcaaaaaa ataatcctct ttgggtcgag cgtcgtaaac ttggtatgaa gcaggtagag
     5221 cttgccagac tggtaaaatg ttcgccttca tatctaagca aggtagaaaa taatcaactg
     5281 gttcctacag atttttttca gtcacagtgt tctaaagtat taaagaaaaa aagatcatat
     5341 cttttccctt ttatacaaaa aaaatgatta taattgatcc aatttttctg ataatatttc
     5401 tttttttatt gctgtctgct tttttttcgg gttctgaaac agcccttttt tcaattacaa
     5461 aatcggaact atataatctt gctttttctg aaaagcgttc cgacaatctg attgcctcga
     5521 taatgaaaaa gcctcgaaag gcattaatca caatattaat tggaaaccta g
//
LOCUS       DAOTGZ010000184         5466 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_3064
            NODE_17711_length_5466_cov_4.078174, whole genome shotgun sequence.
ACCESSION   DAOTGZ010000184 DAOTGZ010000000
VERSION     DAOTGZ010000184.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868088
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 5466)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5466)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:22:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,785
            CDSs (total)                      :: 2,751
            Genes (coding)                    :: 2,732
            CDSs (with protein)               :: 2,732
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 12 of 19
            Pseudo Genes (internal stop)      :: 5 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5466
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_17711_length_5466_cov_4.078174"
                     /isolate="AMR_MDS_3064"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..282)
                     /locus_tag="P5123_10820"
     CDS             complement(<1..282)
                     /locus_tag="P5123_10820"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRX16795.1"
                     /translation="MKKRTKFSISWCDDCAIKRLYRKFMPQSISIQKKIFLLCTLVML
                     ISTVTLSSLFLYITIHKIRQESISQIDIALDFVINDINHKISGLSQNLEN"
     gene            complement(437..1468)
                     /gene="mnmA"
                     /locus_tag="P5123_10825"
     CDS             complement(437..1468)
                     /gene="mnmA"
                     /locus_tag="P5123_10825"
                     /EC_number="2.8.1.13"
                     /inference="COORDINATES: protein motif:HMM:TIGR00420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_function: GO:0016783 - sulfurtransferase activity
                     [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 2-thiouridine(34) synthase MnmA"
                     /protein_id="HRX16796.1"
                     /translation="MNIAVAMSGGVDSTVCASLLKSEGHNVTGFTALMYDGGDVTVHN
                     AKVCAQHIGIEHIVLDLRDEFNKRIIEPFVQSYLNGRTPSPCIRCNRLIKFGLILDSI
                     IDAGIEQIATGHYAAIHRDNKRFYLSQAKDPIRDQSYFLFDLTQEQLKHILFPLANFS
                     KDEIRNIATTHGLPAAKSKDSQEICFIPDNNYRGFIQDRCKTPDQGDITDKSGKSFKK
                     HDGIYRYTIGQRRGLGISHSEPLYVTDIIPEENRIIAGPKTDLFKNGLIAGSINFMKQ
                     SAFNNDNVFAKIRSTQPAIPAKATILENGKLKLIFEQPTMQISPGQAAVLYDNKSNLL
                     AGGWIESAF"
     gene            complement(1484..3850)
                     /locus_tag="P5123_10830"
     CDS             complement(1484..3850)
                     /locus_tag="P5123_10830"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRX16797.1"
                     /translation="MKNIFFIVISFLLVTTYTLKAEERVLWWQNRIDSVSKQRQELEK
                     ESYYPLIADALEYDLKCSRSYLSDLTEEPGTEKKIVNGKSITTLFNEKFNSIFASEYL
                     KTAYKILESNESLEKSGHLLDQSINSKIEIYLEGTTEKERQIITRSAITPQLEKSFKY
                     EMTLLSIISDKPMIMEKTRSMAIKEFTIENSGKPLSRKKAILSLSDHINRALNKVYPA
                     FSFSPKNTNVKQVPSYRDIEWLIDVQLSQINALRSLDKSISVDRAIALLSSPAALDKH
                     FFSATLNTNDTKKSRIITLSLKRQMYTALANYKTAKEAVDSINRSSEAMEAKYKTSLE
                     ESELINNFKGRINEYIKLSFDLKKSDPITAHENIDHRFSTIRRCIKFMEKNLTEGTSE
                     STVFLSNSKAYVEHVRYLSYGIFVLPQYRKVLNNKNLRLSLRNQNELKEFAKGSISTA
                     PSLAKSIKLKMANKEKAIALSLSEDIDNSATSELALLSDIINNYYLTFSSFNNAPDYL
                     SLYIRKYTDMEEKLLKGIITDEIKQCVKNGSIIPSVKASNTVMDNENKAKKFLKDTIA
                     FNIVRFVKINNQYRSKGINVNLPGEIDTLYTIRNKMDDEYSSTVNGWTINENNYTSVD
                     QRLAQRLKKIYRQKVWEKSSDGSLANTGSRKIIWEESGLSFFIPEGWAPLNVINTDER
                     LTASYQNSLDNTKLSIELTTEDSETDAVNNWLRQKGFKAVQLGWEDKDGTMYLWKIGR
                     DKKGNIAKILAYRKKDRIVIVSGISTKKRYPFFHNRIDTVFNSLESFK"
     gene            4138..4848
                     /locus_tag="P5123_10835"
     CDS             4138..4848
                     /locus_tag="P5123_10835"
                     /inference="COORDINATES: protein motif:HMM:NF014045.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NYN domain-containing protein"
                     /protein_id="HRX16798.1"
                     /translation="MRAAIFIDGGYFLNFFKKYDAVPKYEDLADYFLKPLRKTVQLDL
                     LRCYFYYCAPWMSTDPTDTEKRRMEEHDLFMEEIEKLERWSVRLGKLQRRWDGHKEFF
                     EQKRVDVLLSVDMVRHSAAGHIQHAIVVAGDSDFIPAVEATKESGATVTLWHADENTV
                     HKDLLSLADCVNQMNIKKIPREKIKASKKAEKQREMLKKRQTESVAPVPQVQAVPQRS
                     VPKKSNSPRPYNQRKRRG"
     gene            complement(4872..5288)
                     /locus_tag="P5123_10840"
     CDS             complement(4872..5288)
                     /locus_tag="P5123_10840"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRX16799.1"
                     /translation="MPSLHVMIVTWAAHSLVPRITELASDYKNSSDIKEITDYIENRA
                     CLITESILYIKQHSINCIPAALYVMQNLFPGFEDQYEKTFINCLFKTSHPPVKDRGKL
                     ILFMEKLYDKFRNEDPDLNFKQKIINFLNSYPKQYR"
BASE COUNT         1527 a          930 c         1109 g         1900 t
ORIGIN      
        1 gttttcaaga ttttgactta agccggaaat cttgtggtta atgtcgttta ttacaaaatc
       61 tagcgctata tctatttggg atatcgattc ttgtcggatc ttgtgtatgg ttatatataa
      121 aaaaagagac gaaagtgtaa ctgttgaaat aagcattaca agggtacaca gtaaaaaaat
      181 ctttttctga atagaaatag attgaggcat gaattttctg tacaatcttt tgattgcaca
      241 atcatcgcac catgaaatag aaaatttagt tctttttttc atgaatcaac cagtaataca
      301 atcaagtcat tataaaaata atttgcgcag gtttttaaaa acataaaatc ataacctctg
      361 gtatctttct gtcttaatat atccagataa agaggctagg gcaatgtttt tgatctgaaa
      421 atagattatg atgtaattaa aaagctgatt cgatccaacc tccggcaaga aggttgctct
      481 tgttgtcgta gagcacagca gcctgacctg gagatatttg cattgtgggt tgttcaaaaa
      541 tgagtttcag tttcccgttt tcaagaatgg ttgcttttgc cggtatggct ggttgagtag
      601 atctaatctt agcgaagaca ttgtcgttat tgaaagcgga ttgtttcata aaattgatcg
      661 agccagctat gagcccgttt ttaaaaaggt ctgttttagg gccggcaata atacggtttt
      721 cttcaggaat aatgtcggtt acataaagag gctcgctatg ggatatgcca agcccgcgtc
      781 gttgtcctat agtgtaacga taaataccgt catgtttttt gaatgactta cctgatttat
      841 ctgtaatatc tccttggtct ggtgttttgc aacggtcttg tataaagcca cgataattgt
      901 tgtcaggaat aaagcatatc tcttgactat ctttagattt cgctgcaggc aatccatgag
      961 tagttgcaat atttcgaatc tcatctttag aaaaattggc aagaggaaag agaatatgtt
     1021 ttaattgttc ttgtgtaaga tcaaataaaa aatatgactg gtctcgtatc ggatcttttg
     1081 cctgagacag atagaacctt ttgttatcac gatgtatcgc tgcatagtga cccgttgcta
     1141 tctgttcaat tccggcatct attatactgt caagaataag tccgaattta attagcctgt
     1201 tgcatctgat acagggactt ggagtgcggc cgttaaggta actctgtacg aaaggttcaa
     1261 taatcctttt gttaaattca tctctaaggt caagtacaat atgttcgatt ccgatatgct
     1321 gagcgcaaac ttttgcgtta tgtactgtga catcccctcc gtcgtacata agggctgtaa
     1381 aacctgttac gttatgccct tcggatttaa gcagagaagc gcaaactgtt gagtcaacac
     1441 caccactcat tgctacagct atattcatca ttacctcgtt ttgttattta aaactctcca
     1501 gcgagttaaa aacagtgtca atcctgttgt ggaaaaaggg ataccttttt tttgtactaa
     1561 taccacttac tatgacaatt cggtcttttt tgcggtatgc aagtattttc gcgatatttc
     1621 cttttttatc gcgtcctatt ttccaaagat acatggttcc gtctttgtct tcccagccaa
     1681 gttgaacagc cttgaatcct ttttggcgca gccagttatt aactgcatcg gtctcggaat
     1741 cttcagtagt taactcaatg ctgagttttg tattgtccag gctgttttga tagcttgcgg
     1801 taagcctctc atcagtgttt attacgttta gtggagccca accctcaggt ataaaaaagc
     1861 ttagcccgct ttcttcccag attatctttc gactaccggt attggcaagg gaaccgtctg
     1921 aacttttttc ccagaccttt tgcctgtata tttttttcag tctttgtgca aggcgttgat
     1981 cgacgcttgt gtagttgttt tcattaatgg tccaaccgtt tacagtcgag gagtactcat
     2041 cgtccatttt atttctgatt gtgtaaagag tatctatttc gcccgggaga ttgacattta
     2101 ttcctttaga gcgatactga ttgttaattt tgacaaaacg tacaatatta aaggctatag
     2161 tgtctttcaa aaatttcttt gccttgtttt cattatccat gacggtgttg gatgctttta
     2221 cggaaggaat aattgatcca ttctttacac attgctttat ctcatcggta atgatgcctt
     2281 ttaagagttt ttcttccata tctgtgtatt ttcttatgta cagtgataag taatccggag
     2341 cattattaaa agagctaaat gtcaaataat agttatttat tatatctgat agcagggcaa
     2401 gttcagaggt cgcactgttg tctatatctt cgcttaaact aagtgcgatc gctttttctt
     2461 tgttagccat tttaagtttt attgatttgg caagagacgg agctgtagaa atggagcctt
     2521 tggcaaactc cttcagttcg ttctgattgc ggagtgacaa acgaagattt ttattattta
     2581 atacttttct atattgcggc aaaacaaata ttccataact gagatatctg acatgttcta
     2641 catatgcttt gctgttactt aaaaaaaccg ttgattcaga tgttccttct gtcagatttt
     2701 tttccatgaa ttttatgcag cggcgaatgg ttgaaaacct atggtctatg ttttcatgtg
     2761 ctgtgatcgg gtcagatttt tttagatcaa aactcagttt tatatattcg ttaattctac
     2821 ccttgaaatt atttataagt tcagattctt caagcgatgt tttgtatttt gcctccattg
     2881 cttctgagga tcgattaata gaatcaaccg cttctttagc ggttttgtaa ttcgcaagag
     2941 cagtgtacat ttgacgtttt agtgacagag taattatacg actctttttt gtatcgttag
     3001 tatttagtgt ggctgaaaaa aaatgtttat ctaatgctgc gggggaagat aatagtgcta
     3061 tagcgcgatc aacgctgata ctcttatcga gagatctaag cgcatttatt tgagatagtt
     3121 ggacatctat cagccattcg atatctctgt acgatggaac ctgtttcaca tttgtgtttt
     3181 taggcgaaaa gctaaaagct ggatagacct tatttaaggc tctgttaata tgatcactta
     3241 gggataaaat tgcttttttc cgactcaggg gtttacctga gttttcaatt gtgaattctt
     3301 ttatagccat actcctagtt ttttccataa tcatcggttt atcactgata atcgataata
     3361 gagtcatttc gtatttgaag ctcttttcca actgaggtgt gattgcgctt ctggttatta
     3421 tttgtctctc tttttcggtt gtaccttcca aatatatctc tattttgcta ttaatggatt
     3481 gatcaagcag atgtccgctt ttttcaagtg attcgttcga ctcaaggatt ttatatgctg
     3541 ttttcaggta ttcggacgcg aatattgaat taaacttctc gttaaacagg gtggtgatac
     3601 tttttccgtt tactattttc ttttctgttc caggttcttc ggttaaatca gaaagatatg
     3661 atctgctgca tttcaaatca tattcaagcg cgtcggcgat tagtgggtaa tagctctctt
     3721 tttcaagttc ctggcgctgt ttcgaaacag aatcaattct gttttgccac cataatacac
     3781 gctcttcggc ttttaatgta tacgttgtga ccagcaaaaa agagattaca ataaaaaaaa
     3841 tattcttcat aatcgactcc actattgaaa ataacgatac ggatatatac ctttagtaat
     3901 tatcggtcag acagcagaat tgataaagaa aggttttgtt tcactattgt aacagataat
     3961 ctgtttgaca tctctgggtt agtaaggatt actattttaa aaattaatcc gcaaagggat
     4021 cgcaagtttg ttagggagta ttgtatcatt agcggttgta tctggaattt tgcagatttg
     4081 cggtctaatc cgagaaataa taataataat aataataatg tttaagggag tattaatgtg
     4141 cgtgctgcaa tatttataga tggtggatat tttcttaatt ttttcaaaaa atatgatgct
     4201 gtgccaaaat atgaggatct ggccgattat ttcttaaagc ctttacgtaa aactgtacag
     4261 cttgatcttc taagatgcta tttttattac tgcgctccgt ggatgtctac tgatccgact
     4321 gacactgaaa agcgccgtat ggaagagcat gatctattta tggaagagat tgaaaagcta
     4381 gagcgctggt ccgtgcgttt gggaaagctt cagcgaaggt gggacgggca caaagaattt
     4441 tttgagcaaa agcgggtaga tgttctgctt tctgttgata tggtgcggca ctctgcagct
     4501 gggcatattc agcacgcaat agtagtagca ggggacagtg actttatacc tgcagttgag
     4561 gcaacaaaag agtcgggggc aactgttacc ttatggcatg cagatgagaa cacagtacat
     4621 aaggatctgc ttagtcttgc tgattgtgtt aatcaaatga atatcaaaaa gataccaaga
     4681 gagaagataa aggcttccaa gaaagcggaa aagcagcgcg aaatgttaaa gaaaaggcaa
     4741 acagagagtg ttgcacctgt accacaggtg caagctgtac cacagcgttc tgttcccaaa
     4801 aagagtaatt caccccgtcc ctataatcaa cgaaaaaggc gtggttaatt ttataccaat
     4861 atttttagtt actacctgta ctgttttgga taactgttta aaaagttaat aattttctgc
     4921 ttgaagttca gatctggatc ttcattgcga aatttgtcat aaagtttttc cataaacagg
     4981 atcaactttc cccgatcctt aaccggcgga tgacttgttt tgaataggca attaatgaaa
     5041 gttttttcgt actgatcttc aaaacctgga aaaagattct gcataacata gagagccgca
     5101 ggtatgcagt tgatgctatg ctgtttgata tatagaatac tttctgtaat cagacaggct
     5161 ctgttttcaa tgtaatctgt gatttcttta atatcagaac tgtttttata atcggaggct
     5221 agttctgtta ttcggggaac tagggaatgg gctgcccatg taacaatcat aacatggagg
     5281 ctgggcatac agtttaaatg aggatccatc ttatgtatca gggcgaatgt gcggtcgccc
     5341 tttacaggtg cgggtggtgt tgttgtggat tgggattttc tgtatgtgga acctgcggta
     5401 gggtagataa gtttaagttt tttgtgtgat ttttataccg aaataaaaga tttgtattta
     5461 catttg
//
LOCUS       DAOTGZ010000185         5429 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_3064
            NODE_17856_length_5429_cov_4.972460, whole genome shotgun sequence.
ACCESSION   DAOTGZ010000185 DAOTGZ010000000
VERSION     DAOTGZ010000185.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868088
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 5429)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5429)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:22:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,785
            CDSs (total)                      :: 2,751
            Genes (coding)                    :: 2,732
            CDSs (with protein)               :: 2,732
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 12 of 19
            Pseudo Genes (internal stop)      :: 5 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5429
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_17856_length_5429_cov_4.972460"
                     /isolate="AMR_MDS_3064"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1224
                     /locus_tag="P5123_10845"
     CDS             <1..1224
                     /locus_tag="P5123_10845"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="CoA activase"
                     /protein_id="HRX16800.1"
                     /translation="SKNKNIVNDKILLMPCWDHINSRLMQARLIQEGIDARLIPDTIE
                     SIQRSLQHNTGQCIPLNIIAQNAMEYVRQNNLDPAKCVLWMFDSSISCNVRMYPYYLR
                     KLMQQQGGGMENLAVYIGELTFTDFSYSTAIDIYFAHMFGGLLRKMGCRLRPYENIPG
                     STDKAIEESTEILFNMFKTGSDKEEALEKVISLLEKIDITPGSKPKVALFGDLYVRDN
                     DIMNQQLIKTIEQNGGEVVTTPYNEYLKLIASPYIRKWITEGLYSNAALGKIMITVVN
                     MIEKKYSKYFSRILKEEIETHDISAETLLDKYNIKPEHTGESMDNILKICTIAERYPD
                     LALFVQTNPAFCCPSLVTEAMSQRIEADTGIPIITIEYDGTGGNKNDVLIPYLRFPRM
                     ARTEKSQNHNEANAG"
     gene            1230..1949
                     /locus_tag="P5123_10850"
     CDS             1230..1949
                     /locus_tag="P5123_10850"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRX16801.1"
                     /translation="MKMTNLIISLMLTLSIIGIFTEDITPNKPALQLAGFGEALDQLK
                     TSLDRLKTSLDRLKTSLERTEQFITKIYETVKAITSFAGLKTIIVLIITFGIASGIGT
                     TGIAKGKNIFILSLLIVDLLWFFWFGVFYDEKKELLRDMIKSNLLILSPFLLIILLKY
                     IMPPIYRFTRSFNRKNVHTKKFCIKHWNEFNTESSNFITNYNNDIHEANNSVVLSADT
                     TRSIKKLMILLKELGSKEKSV"
     gene            1959..2723
                     /locus_tag="P5123_10855"
     CDS             1959..2723
                     /locus_tag="P5123_10855"
                     /inference="COORDINATES: protein motif:HMM:NF013213.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TatD family hydrolase"
                     /protein_id="HRX16802.1"
                     /translation="MQIFDAHAHLSNVTTTPHLQTANNSDLPLPDYIVCNSTQPQDWS
                     QTIDTCRLDKRLLPALGIHPWYTNNDYMLYLDKLSLYMSDLHAIGETGLDKLRGNFTL
                     QKASFIEHIRLADHLGLTLVVHCVKAFDELYAIISKEKPKTSILIHGYNKSKQLAQQL
                     CTICDCYFSLSPLFFETNNGISEKKRQLLDYLILSNRLLLETDFHFYYYKKEGIEEYQ
                     NLIQDSLNKITLVLKKDKATLSKNIFALSCKLFKAD"
     gene            complement(2775..4787)
                     /locus_tag="P5123_10860"
     CDS             complement(2775..4787)
                     /locus_tag="P5123_10860"
                     /inference="COORDINATES: protein motif:HMM:NF014763.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein"
                     /protein_id="HRX16803.1"
                     /translation="MVNKLVTNKSIGTRLTGGVIGMLICVSIGVGSVGYWQAAKALKG
                     QVEEVIPQTAKNAASIVRAILDRHITTVTQFAETPYIKNLNPDEQMVVLKQANRSGFM
                     QLGFFDTNGDVTLSDGSKTNIKDRPYFGDTIAGKTHASDIFIHRILKIPVMTISVPVR
                     NGNGVIVGGVLAVMKAEWLSMTVDSLGYGEKGYAYIIDKTGTLIAHGNRDFVNNQRNF
                     LEEGKTNPEFAKLSVMFEKMVKGEMGFDEYPFMGSVRFFGYSPIPDSSWSIAVGAHRS
                     DVFHQLPFMRFMVIILTLFFILLGIVVTFIISRSIVQPITAAANYANVLEQGDFTIDI
                     DNRHFERKDETGVLMESLHNMRVKLSDVVRSIQNLASELAVSSEEMSGAAVSFSDNAQ
                     NQAASAEQITATVEEVSAGIENVALHSHSQHDKLDQFEQQMQSLSEIIQQTGEKLDET
                     ASILNQIVKKAQMGEKSLTRMSQSMTKITNSSHEMVGIVAMINDISEQINLLSLNAAI
                     EAARAGDSGRGFAVVADEVSKLAEQTASSIKEIDRNIQINNEEIENGMGSVAEAISTI
                     SEIISGVNNVSQMTNLLVGYMDTQMNTKNRVLRETVSMKERSSEMKSATEEQKVAVGE
                     VVNSISIINELTQGNASGAEEMAGTSESVSSIAEKLKSAVGFFKIS"
BASE COUNT         1713 a         1117 c          918 g         1681 t
ORIGIN      
        1 tctaaaaata aaaatatagt aaatgataaa atcttgctta tgccctgctg ggatcatata
       61 aattcacgtc ttatgcaggc caggttaata caggaaggta ttgacgcacg acttatacct
      121 gacacaatag aatctattca gagaagtttg cagcataata ccggtcaatg cataccgcta
      181 aatataattg cccaaaacgc aatggaatat gtacgtcaaa ataatcttga tcctgcaaaa
      241 tgtgttctct ggatgtttga ttccagtatt tcgtgtaacg ttagaatgta tccttactat
      301 ctgcgcaagc ttatgcaaca acagggcggt ggaatggaaa accttgctgt atacatcgga
      361 gaattaacat ttactgattt ttcttattca actgcgattg acatctactt cgctcatatg
      421 tttggcggat tgttaagaaa aatgggttgt cgtttacgac cttatgaaaa tattccagga
      481 agcactgaca aggcgattga agaatctaca gaaattctat tcaatatgtt taaaacaggt
      541 tcggacaaag aggaggcctt ggaaaaagtt atttctctgc tagaaaaaat agatataact
      601 cccggatcaa aacccaaagt cgccctcttc ggagaccttt acgtacgtga taacgacatt
      661 atgaaccagc agcttattaa aactatcgaa caaaacggcg gagaggtggt tactacaccc
      721 tataacgaat atttaaaact catcgccagt ccttatatcc gcaaatggat cacggagggt
      781 ctctactcca atgcagcgct tggtaaaata atgattaccg ttgtaaacat gatagaaaaa
      841 aaatattcca aatactttag ccgaattctt aaggaagaga tcgaaacgca tgacatatca
      901 gccgaaactc tgcttgataa atataatatt aaacccgagc ataccggtga atccatggac
      961 aatattttaa aaatatgcac aatagccgag cgatatcctg atcttgccct ttttgtgcaa
     1021 accaacccag cgttctgctg tccctctctt gtaactgagg ccatgtcgca gcgtatagaa
     1081 gccgatacag gaatacctat aataacgatt gaatatgatg gaactggcgg taataaaaac
     1141 gatgtgctta taccttacct tcgttttccg cgaatggcaa gaaccgaaaa gagtcaaaac
     1201 cataatgaag caaacgcggg gtaaattata tgaaaatgac aaatttaata atatcattaa
     1261 tgctaacttt atcaataatc ggtattttca ctgaagatat tacccccaac aaaccagcac
     1321 ttcaactagc cgggtttggg gaagcacttg atcaacttaa aacttctctt gatcgactta
     1381 aaacttctct tgatcgactt aaaacttctc ttgaaagaac cgagcagttt atcactaaaa
     1441 tttatgaaac ggttaaagca ataacatctt ttgccggtct taaaactatc atagtactta
     1501 ttattacttt cggaatagca tctggaattg gcacaacggg tattgctaaa ggcaaaaaca
     1561 tctttatact gtcactcctt attgtcgatc tcttgtggtt tttttggttt ggagtatttt
     1621 atgatgagaa aaaagaactt ctcagggata tgattaaatc aaatcttctt atcctttcgc
     1681 cttttttact cataatactt ttgaaataca taatgccacc gatttacaga ttcactcgct
     1741 cttttaacag aaagaatgta catactaaga aattttgtat caaacactgg aacgaattta
     1801 acacggaatc atctaatttt attacaaatt ataataacga tattcatgag gctaacaata
     1861 gtgttgtgtt atcggctgat acaacacgca gcataaaaaa actgatgatt ttacttaaag
     1921 aacttggcag caaagaaaaa agtgtatgac agcagtaaat gcaaattttt gacgcccatg
     1981 cgcatcttag taatgtaact actacacctc atttgcagac ggctaacaat agtgatctgc
     2041 cattaccaga ttatattgtc tgcaattcta ctcagcctca ggattggagc cagacaattg
     2101 atacttgccg tcttgacaag aggcttttgc ctgctttagg cattcatcct tggtatacga
     2161 ataacgatta catgctctac cttgataaat tatctttata tatgtccgat ctgcatgcaa
     2221 ttggcgaaac aggcctggac aaattgcgcg gaaactttac tctgcaaaaa gcttctttta
     2281 tagagcatat tcggcttgca gatcatttag gtctcaccct tgttgtccac tgcgtaaagg
     2341 cttttgacga gctttatgca ataataagta aagaaaaacc taaaacatca attttaatcc
     2401 atggatataa taaatcaaag cagttagccc agcaactatg cacaatatgc gactgctatt
     2461 tttcgttatc acctctattt tttgaaacaa acaacggtat cagcgaaaaa aaaagacaat
     2521 tactggatta tctgatatta tcaaacaggc tacttcttga aacagatttt catttttatt
     2581 attataagaa agaaggcatc gaggagtacc aaaatcttat tcaagatagc ttgaataaga
     2641 tcacactggt attaaaaaaa gataaagcta ccctatcaaa aaatatcttc gctctctctt
     2701 gcaagctttt taaggcagat tgaagagcca aattatttta ctatacgata aaactttttt
     2761 agataacctt cattttagct tatcttgaag aaaccgacag cacttttaag tttttcagca
     2821 atagaagata ctgactccga tgtcccagcc atctcttccg cgcctgatgc gttgccttgg
     2881 gtaagttcgt taattatgga aatagagtta acaacttcac caacggccac cttctgttct
     2941 tctgtagcgc ttttcatctc ggaagatcgc tctttcattg aaacagtctc tcgtaaaacc
     3001 ctatttttag tgttcatctg ggtatccata taccccacta aaaggttggt catttgactt
     3061 acattattca caccagaaat aatctcgctg attgtactga ttgcttcggc aacagagccc
     3121 ataccgtttt cgatctcttc attgttaatc tgtatattgc gatctatctc tttaatgcta
     3181 gaagctgtct gttcggccag ctttgacacc tcatccgcta ccaccgcgaa accacgccca
     3241 gaatcaccgg cacgcgccgc ctcaatagcc gcgttcaatg ataacagatt gatctgttcg
     3301 gaaatatcgt taatcatagc aacgattcct accatctcgt gagagctatt ggttatcttg
     3361 gtcatactct gcgacattcg agtaagtgac ttttcaccca tttgggcctt cttgacaatc
     3421 tggtttaaaa ttgaagctgt ttcatcaagc ttttcacctg tctgttgaat aatttctgag
     3481 aggctttgca tctgctgttc gaactgatca agtttgtcat gctggctatg tgaatgaagt
     3541 gcaacatttt cgattccggc cgaaacctct tcaactgtgg ccgtaatctg ttctgcagag
     3601 gcagcctgat tttgcgcatt atcagaaaag gatacagctg ctcccgacat ctcttcagat
     3661 gatacagcca gttcagatgc aagattttga atacttcgaa caacatcact caacttgaca
     3721 cgcatattat ggagagactc cattaatact ccagtttcat ccttacgctc aaaatgcctg
     3781 ttatcgatat cgattgtaaa atcgccctgc tcaagaacgt tagcgtaatt tgctgctgct
     3841 gtaataggtt gcacaatact tcttgaaata ataaaggtta caactatacc taaaagaata
     3901 aaaaacaggg taaggataat taccataaac ctcataaacg gtagctgatg aaatacatca
     3961 ctccgatgag cacctaccgc tattgaccaa ctgctatcag gtattggaga gtaaccaaaa
     4021 aaacgtactg aacccatgaa aggatactca tcaaacccca tttccccttt taccattttt
     4081 tcaaacatta cagaaagttt ggcaaactca ggatttgttt taccctcttc aagaaaatta
     4141 cgctgattat ttacaaaatc cctgtttcca tgagcgataa gagttccggt tttatcaatt
     4201 atatatgcat agcctttttc accataacca agtgaatcaa ccgtcatgct tagccactct
     4261 gctttcataa cagccagaac tcctccaaca ataactccgt taccatttct cacaggcact
     4321 gaaattgtca taacaggtat ctttaaaatg cgatgtataa aaatgtcaga ggcatgtgtt
     4381 ttgcctgcta ttgtatctcc aaaatatggt ctatccttta tatttgtctt agaaccatca
     4441 ctaagagtaa catctccatt tgtgtcaaaa aaaccgagtt gcatgaaacc ggatctgttg
     4501 gcctgcttta atactaccat ctgctcatcg ggattcaggt tttttatata tggagtttca
     4561 gcaaactggg taactgtcgt tatatgcctg tcaagtatag ctcttacaat agaagcggca
     4621 ttttttgctg tttgaggaat tacctcctcg acctggccct ttaaagcctt agctgcctgc
     4681 cagtaaccaa cgcttccgac gcctatggaa acacagatta gcattccgat tactccgccc
     4741 gttaagcgtg tcccgattga tttattagtg acaagcttgt taaccatttt tgcactctcc
     4801 gtgattgttt agcagtttat ttgatatcat tattgatctc atttgagcat ataaaaaaat
     4861 ggcgaaactc aatgccttta actgtacaat tttacaaatt ttagtatttc cgtgaaatct
     4921 tgcagctaat cattacggga acttctaaaa attaccattt tcggtacggc tatgccattt
     4981 aacttattat aaggataatt tttcgctatt atcttgttta taaactgatt attagcggat
     5041 cccttaactt aaattgcgac ttaactctga taccaataat ttaacgaaac aattgacgca
     5101 atacactata ttgtcggttt ttcatacatc ttttgttaat aatgttttgt ccaatatttt
     5161 ttataatagg gaacttcgaa aaattaccat ttctggaacg gctataccgt ttaaagccaa
     5221 attaaagtta atttttggag attgccaata tatattgcaa cttgatcatt agaagcatga
     5281 aatttacctg atagatatcg ggtaaatatt caatcaatat agtcctcaac ccggcaaatt
     5341 atttccaggt cgaggactat acaaaaatat tcctacttaa atgagctgcg gttatgctct
     5401 ctgattattt tggcaagagc cttgtctcg
//
LOCUS       DAOTGZ010000186         5372 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_3064
            NODE_18121_length_5372_cov_4.837879, whole genome shotgun sequence.
ACCESSION   DAOTGZ010000186 DAOTGZ010000000
VERSION     DAOTGZ010000186.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868088
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 5372)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5372)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:22:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,785
            CDSs (total)                      :: 2,751
            Genes (coding)                    :: 2,732
            CDSs (with protein)               :: 2,732
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 12 of 19
            Pseudo Genes (internal stop)      :: 5 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5372
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_18121_length_5372_cov_4.837879"
                     /isolate="AMR_MDS_3064"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..804)
                     /locus_tag="P5123_10865"
     CDS             complement(<1..804)
                     /locus_tag="P5123_10865"
                     /EC_number="1.3.1.14"
                     /inference="COORDINATES: protein motif:HMM:NF005574.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004152 - dihydroorotate dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006221 - pyrimidine nucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotate dehydrogenase"
                     /protein_id="HRX16804.1"
                     /translation="MEKSKKLSVKFLNCKSDSPLVLPAGIMDVSYGSMLFAVQNGAGF
                     VTMKSCTLEPRSGHAGPVVCEIEGAMINAMGLCNPGIEKGLEEIEKFKKESDVPVISS
                     VFGANRDEFVKLSDYVNDSSADYIELNLSCPNVSSEFGTPLSASKEMVADIVGAVKKN
                     SKLPVLAKLSPNTHDVTGIARAAENAGADGLVMINTLGPGMVIDINARRPVISAKFGG
                     ISGPAVKPVAVKLVYQVTEAVDIPVIGMGGVTTGSDAIEMMMAGASLVGV"
     gene            complement(887..1339)
                     /gene="pyrI"
                     /locus_tag="P5123_10870"
     CDS             complement(887..1339)
                     /gene="pyrI"
                     /locus_tag="P5123_10870"
                     /EC_number="2.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014122648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004070 - aspartate carbamoyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009220 - pyrimidine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate carbamoyltransferase regulatory
                     subunit"
                     /protein_id="HRX16805.1"
                     /translation="MKELKVDAIKCGTVIDHIPPGKVFKVIDLLGIGENDQVMIGTNL
                     SSPKHGRKDVIKIENRYLSIEEINSVSLVARSATISIIEDFKPCKKTKIEIPDVLKGI
                     LVCPNRHCVTNSEPVITVFDVEAKDPLSIRCRYCERVFGEEIGKANSL"
     gene            complement(1339..2283)
                     /gene="pyrB"
                     /locus_tag="P5123_10875"
     CDS             complement(1339..2283)
                     /gene="pyrB"
                     /locus_tag="P5123_10875"
                     /EC_number="2.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013905494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004070 - aspartate carbamoyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009220 - pyrimidine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate carbamoyltransferase"
                     /protein_id="HRX16806.1"
                     /translation="MKQENQTPGQGGAMDIISMRSLSKDNVTSLIKLTEKIESGSISL
                     DISGHLVALLFFEPSTRTAFSFDSAIKRLGGQTIIMQETKSSSVQKGESFADTIKTVS
                     QYADAIVIRSAIEGAARFASEIVNIPVINAGDGANQHPTQTLLDLYTIKKQQKTVENR
                     KVAIVGDLRYGRAVYSLVYGLAMFNAELYLISPEHLKIRAYVRHDLDKMGIKYHESED
                     ISAVLDKVDVMYVTRIQRERFAGQYEYEKVKDSYQITRSMVESASLKNNFTLLHPLPR
                     VNEITTDVDSLPCAHYFAQAKNGMYMRQAIIASLLGVV"
     gene            2563..3342
                     /locus_tag="P5123_10880"
     CDS             2563..3342
                     /locus_tag="P5123_10880"
                     /inference="COORDINATES: protein motif:HMM:NF013700.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan DD-metalloendopeptidase family
                     protein"
                     /protein_id="HRX16807.1"
                     /translation="MFFRVTTVFMLILFLLPIFANERNVSINRRPSGIKESYLKSKGL
                     SPATENGKKSANKTDKNSVKKSSTKSETNTKVLIHTIKKGENLTKIARKYNTTAANIL
                     SLNKIKNPNKLYVGTRLKIPSTKSLSSTNTSKPAAKPISCKLKFIWPAKNIARVVSDK
                     KGTANPIGITIFSGKKTSVICSENGIVKKIGYMRGFGNYIVIGHTERFMTVYANIDSV
                     MVKEGEKVKKGAPIATLTSSLHFQINQGGKPLDALTVLPKR"
     gene            complement(3352..4272)
                     /locus_tag="P5123_10885"
     CDS             complement(3352..4272)
                     /locus_tag="P5123_10885"
                     /inference="COORDINATES: protein motif:HMM:NF016289.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M28 family peptidase"
                     /protein_id="HRX16808.1"
                     /translation="MRLFLKLYDTFTGRDKIVVNCRHIVRALSDYIGERSLRLNENLE
                     RAAEFIESAFALHGTIPDKEKFKAEGHNVVNVIAELPGKKNPEKIIIIGAHYDTVEGS
                     PGANDNGSGIAALLELHRLLKKETHKKTIRFVAFALEEPPFFGTELMGSFQHAKNCKK
                     RKENIELMLCLDMLGIGSRFAKQNYPVEDMKRNYPKKGNYLAVATLPSQSKTAFYFKK
                     YFNKNSFIKMFEMIAPASVPGIDYSDHSSFIKQGYPAIMVTDTGFYRNPRYHTSEDIS
                     STLNYRFLGYTVYSLYKTIKDLASGRKIPN"
     gene            complement(4275..>5372)
                     /locus_tag="P5123_10890"
     CDS             complement(4275..>5372)
                     /locus_tag="P5123_10890"
                     /inference="COORDINATES: protein motif:HMM:NF013100.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding transpeptidase
                     domain-containing protein"
                     /protein_id="HRX16809.1"
                     /translation="VQSYRQPGSSVKPLLYAAAVESRQFTAATAVLDAPIMYLDGDGK
                     TWSPENYEGSYSGEVRLRRALAKSINVISVRIAETIGIGTVMDYYAKLFGMDKESADK
                     RINRNLSIALGTVEVTPLEMARSYAVMANGGKAVIPYGIRYIKNREGTVIENREEEVK
                     KELEELTKKKKIQLIEPATASIMISMMKTVVEAGTGVSANPGRPAAGKTGTTNNWRDA
                     WFVGFTPELTTSIWIGYDSLGMSLGRGQTGGNVAAPLWGAYMRGALEKYPVKDFPHYA
                     ELEHAIIARESGLRPGSGCTDVMEEIFIPGTVPTEIDTMCGSYSSGRELLSREPEEDI
                     TASHKVMIDREIGSTAPEESSIADIGLDLLD"
BASE COUNT         1597 a         1213 c          954 g         1608 t
ORIGIN      
        1 gacccccacc agagaggcgc cggccatcat catctcaatc gcgtcacttc cggttgtaac
       61 accgcccatt cctattacag gtatatctac agcttctgta acctgatata ccagctttac
      121 tgcgacaggt ttaacagcag gtccagatat tccaccaaat tttgccgata taaccggccg
      181 tcttgcattt atatctatta ccatacctgg gcccaatgta ttaatcatca caaggccatc
      241 ggcccccgca ttctccgcag cacgagcaat tcctgtaaca tcatgtgtat tgggagatag
      301 tttggctaat actggtaact tggaattttt tttaacagcg cctactatat ctgccaccat
      361 ctctttagat gcagacaaag gagtaccaaa ctctgacgaa acattagggc atgacaggtt
      421 aagctcaata taatcagcag atgagtcatt tacataatcc gatagtttta caaactcgtc
      481 tctgttcgcg ccaaagacac tcgaaatcac agggacatcc gactcttttt tgaatttttc
      541 gatctcttca agcccttttt ctatgcccgg attgcagagc cccattgcgt ttatcattgc
      601 accttcgatt tcacagacaa caggtccagc atggccactt ctaggttcaa gagtacatga
      661 tttcattgtt acgaaaccag caccattttg taccgcaaaa agcattgaac cgtacgagac
      721 gtccattatg cccgctggta aaacaagagg cgaatcagat ttgcaattaa gaaatttaac
      781 tgatagtttt tttgattttt ccatagacct tgaatgtata ttgattattt tgtaatatgc
      841 caaattgagg actaattatc tttttttata tgtttaaaat ttccttttac aaagaatttg
      901 ccttacctat ctcttctcca aaaacgcgtt cgcaatacct acaacgtatc gaaagaggat
      961 cttttgcttc aacatcaaaa acagttatga ccggttcact gtttgtgaca cagtgccggt
     1021 tagggcaaac taaaattcct tttagtacat cgggtatttc tatttttgtt tttttgcatg
     1081 gtttaaaatc ttctataatc gaaattgtgg cactccttgc cactagtgaa accgagttta
     1141 tctcttcaat agatagatat ctattctcaa ttttaattac atctttacga ccatgtttcg
     1201 ggctacttaa gtttgttccg atcatcactt gatcgttctc tccaatccca agaagatcaa
     1261 taaccttaaa cactttcccc ggtggaatat gatcaataac agttccacac ttaatcgcgt
     1321 caacttttaa ctcttttatc atacaacccc caaaagagag gcaattattg cctgcctcat
     1381 atacatacca ttttttgcct gggcaaaata gtgcgcacaa ggtaaagagt caacatctgt
     1441 tgtaatttca tttacacgag gaagaggatg caaaagtgtg aaattattct ttaaactggc
     1501 cgactctacc attgatcgag taatctgata cgagtccttt actttttcat attcatattg
     1561 accggcgaat cgctcacgct gtatacgagt aacgtacatg acatccacct tgtcaagaac
     1621 cgcactaata tcttcacttt catgatactt gatacccatt ttatccagat catgacgcac
     1681 ataagctcgt attttaaggt gttccggcga gataaggtat agttcggcat taaacatggc
     1741 tagaccataa accagagagt aaaccgcgcg gccataacgc aaatcgccaa caatcgccac
     1801 ctttcggttt tcaacggttt tttgctgttt ctttatggta taaagatcaa gtaatgtttg
     1861 agtcggatgt tgattagccc catcaccagc atttataacc ggaatattaa ctatttccga
     1921 ggcgaatcgt gcagctcctt cgattgccga ccttattacg attgcgtcgg catactggct
     1981 cacagttttg atggtatcag caaaagactc acctttttgc acagatgaac tttttgtctc
     2041 ttgcattatt atggtttgcc cccccaatct tttaattgct gaatcaaaag agaaagctgt
     2101 tcttgtggaa ggttcaaaaa acaaaagagc gaccaaatgg ccgctaatat ccaaactgat
     2161 cgaaccggat tcgatctttt ctgtaagctt gattaatgaa gtgacattat ctttggaaag
     2221 agaacgcatc gagatgatgt ccatagcacc tccctgccct ggcgtttgat tctcttgttt
     2281 cacacccccc ctttttgtca aagaaaaatt agccgatacc ctatttttta cataatcttt
     2341 ctttatatca taaaaaaacc gctctcttct ttaacgatta attaaatttg atttatatgg
     2401 tgtccgatat tcataatacg tctaaaatat tgtcagataa aagaagatta ccaatttttt
     2461 tataatcaac ttatccggta tgaccttaac caattgtacc gattctttgt tgatttcgct
     2521 gtatactgtt tattgcaaaa aaaatcagtg gggttactta tagtgttttt cagagttaca
     2581 actgttttta tgcttatcct ttttttactg cctatatttg caaacgagcg caatgtctcc
     2641 ataaacagac ggccatccgg cataaaagag tcatatttaa agtctaaagg cctatcccct
     2701 gccacagaaa acgggaaaaa atctgctaat aaaacagaca aaaatagcgt caaaaaaagc
     2761 agtactaaat cggaaactaa tacaaaggtt ttaatacaca ctataaaaaa gggagaaaat
     2821 cttaccaaaa ttgctcgcaa atacaacaca acagctgcta acatactctc actaaacaag
     2881 atcaagaatc ccaataagct ttatgttggc acccgattaa agataccatc cacaaaaagc
     2941 cttagttcaa caaacactag caaaccagct gcaaagccga tttcatgcaa acttaaattt
     3001 atctggcccg ccaaaaatat agctcgtgtt gtgagtgaca aaaaaggaac agcgaaccct
     3061 atcggaataa caattttttc gggcaaaaaa acatcggtaa tatgttcaga aaacggaata
     3121 gtcaagaaaa ttggctatat gcgaggattc ggcaactata ttgttatcgg tcatactgag
     3181 cgctttatga ctgtatacgc aaatattgat tctgtgatgg tcaaagaagg agaaaaggta
     3241 aaaaaaggag cgccaatcgc aacattaaca tcttctctgc attttcaaat aaaccagggc
     3301 ggaaagcctc ttgatgccct aactgtactg ccaaaaaggt aataaaattg attagtttgg
     3361 aatctttcgc ccacttgcca aatcttttat agttttgtaa agagaataga cagtatagcc
     3421 caagaaacgg tagtttaagg tagacgagat atcttcactt gtatgataac gtggattacg
     3481 gtaaaaaccg gtatcggtga ccattattgc cggatacccc tgttttataa acgaagagtg
     3541 atcagaataa tcgataccag gaacagacgc gggcgctatc atttcgaaca tctttataaa
     3601 agaattcttg ttaaaatatt ttttaaaata aaaagctgtt ttagattgcg atggaagtgt
     3661 cgcaactgca aggtaattac cttttttggg gtaattgcgc ttcatatctt ctacaggata
     3721 attctgtttt gcaaacctgc ttccaatacc taacatgtca aggcataaca tcaactcaat
     3781 attctcttta cgctttttac agttttttgc gtgctgaaaa gatcccatca actctgtccc
     3841 aaaaaatggg ggttcctcaa gagcaaaagc cacaaatctt atggtttttt tatgggtctc
     3901 tttttttaaa agacgatgca attcaagaag agccgctata ccagatccgt tatcgtttgc
     3961 tccgggtgac ccctcaaccg tgtcgtagtg agcaccgatt atgattattt tttcaggatt
     4021 ttttttgcca gggagttctg cgattacatt caccacattg tggccttcag ctttaaattt
     4081 ctctttatcg ggaattgtgc catgaagagc aaaagccgat tcaataaatt ccgcagcacg
     4141 ctcaagattc tcgtttaagc gcaacgatcg ctcacctata taatcggata aggcacgtac
     4201 aatatgccgg caattaacaa caatcttgtc tcttcccgtg aaagtatcgt atagttttag
     4261 aaatagcctc ataatcaatc caacagatcc aaaccgatat cagctattga actctcttca
     4321 ggagcagtac tgcctatttc ccggtcaatc atgaccttat gtgaagctgt gatatcctct
     4381 tcgggttcac gggataaaag ttcacgacca ctactgtaag aaccgcacat ggtatcaatt
     4441 tctgtaggca ctgttccagg aataaatatc tcttccataa catccgtaca ccctgatccg
     4501 ggccgcagtc cgctctcgcg agcaataatc gcatgttcaa gttcagcgta atggggaaag
     4561 tcttttactg gatatttttc caacgctcct ctcatatacg cgccccaaag gggagccgct
     4621 acattaccgc ccgtttgtcc ccggcctaga gacattccaa gactgtcgta accaatccag
     4681 atactggttg tcagttcggg ggtaaacccg acaaaccagg catcacgcca gttatttgtt
     4741 gtaccagttt ttcccgctgc cggccgacca ggattcgctg aaactcctgt accagcctca
     4801 actacagttt tcatcatgct tatcattata gaggcggtag ccggttcaat cagctgaatt
     4861 ttcttctttt tagttaactc ttccagctct tttttaacct cttcctcccg attctcgata
     4921 acagttccct cacggttttt gatataacgt attccataag gtataacagc ttttccaccg
     4981 tttgccataa cagcatacga acgtgccatc tcaagaggag taacctcgac cgttcccagc
     5041 gcaatggaaa gatttcggtt tatacgctta tcggcagact ctttatccat tccaaaaagt
     5101 tttgcgtaat aatccataac tgtcccgatt ccaatggtct cggcaatacg gactgaaatc
     5161 acgttaattg atttagccaa agcacgtcta agacgtacct cacccgaata actgccctca
     5221 tagttttcag gcgaccatgt ttttccatca ccgtcaaggt acattatagg agcatcaagt
     5281 acagctgttg cggcggtaaa ctgtcttgat tcgacagcag cagcataaag aaggggctta
     5341 accgatgagc cgggttgtcg gtatgattgt ac
//
LOCUS       DAOTGZ010000187         5337 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_3064
            NODE_18280_length_5337_cov_4.265051, whole genome shotgun sequence.
ACCESSION   DAOTGZ010000187 DAOTGZ010000000
VERSION     DAOTGZ010000187.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868088
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 5337)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5337)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:22:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,785
            CDSs (total)                      :: 2,751
            Genes (coding)                    :: 2,732
            CDSs (with protein)               :: 2,732
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 12 of 19
            Pseudo Genes (internal stop)      :: 5 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5337
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_18280_length_5337_cov_4.265051"
                     /isolate="AMR_MDS_3064"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            179..913
                     /locus_tag="P5123_10895"
     CDS             179..913
                     /locus_tag="P5123_10895"
                     /inference="COORDINATES: protein motif:HMM:NF012257.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0030552 - cAMP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HRX16810.1"
                     /translation="MKTEIIDTEKKSLLSLMKANYRLKYVQEEDLLELLSYCHFRKFS
                     KGSYVFMEGDDVESLYIVSSGKIELNMNNHKFQEKIFSIIGPGQLLGLSEIFNCHQIH
                     TTNALCEQDCTVAAISRDHFRSIITALPSLSFAVCIIMGNMIGELRHELSLSSAEVKI
                     MSYLKNLMIKSAVLKDGQIIVPREVRYEKLAKILNITRETTSRVFISLKDRHIIDIHK
                     DYYIILDEEKIMNIVPHYSCLSQSYN"
     gene            complement(940..1788)
                     /locus_tag="P5123_10900"
     CDS             complement(940..1788)
                     /locus_tag="P5123_10900"
                     /inference="COORDINATES: protein motif:HMM:NF021252.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C-GCAxxG-C-C family (seleno)protein"
                     /protein_id="HRX16811.1"
                     /translation="MKSDKTKQASPRMVFWRRGACSTAMCHLLNREYNNDRHAEEEAL
                     AILAGGIALKGQQCGMLWGASLACGIESYQRYGNSDTAIKAAIDASKNIIESFQKRAG
                     SINCQDITNTDWQNRWQFFTYMFKVIIRGFVFSPCFNLIARWTPEALVSADEGLTNQA
                     KCKPSCVSCSSEVLKKMGASEEDTTAAAGFAGGIGLSGYACGALSAVIWYKMLLWCRD
                     NSGQTPSYFNNPVTKKVLSAFYSQTDSEMLCNKITGKHFDSVEQHSEFIKNGGCDKLI
                     SALSAL"
     gene            complement(1841..2074)
                     /locus_tag="P5123_10905"
     CDS             complement(1841..2074)
                     /locus_tag="P5123_10905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018983392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HRX16812.1"
                     /translation="MKNKCTISNKIREHRFFANEMTQQQLAEKTGVSRQTIIAIEAGK
                     YSPSLELAFKIANAFDVKIDQVFSFKVEVNQKD"
     gene            complement(2082..2573)
                     /locus_tag="P5123_10910"
     CDS             complement(2082..2573)
                     /locus_tag="P5123_10910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014270988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRX16813.1"
                     /translation="MAYKEKKTIIMIVTGIAVLTAYCYYSFGCSTAATDTDAILKQQA
                     ITMLKFIGIGIVAMIVIQILFHILYSISLAIASQIKTGNCDDKDIEKMIELEVVEDER
                     DKIIELKSLRVGFTFAGIGFISALVWIVMDGSAAYLLNIIFISFSLGSILEGFTRLYY
                     YSR"
     gene            2811..3101
                     /locus_tag="P5123_10915"
     CDS             2811..3101
                     /locus_tag="P5123_10915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009224871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-sensitive transcriptional regulator"
                     /protein_id="HRX16814.1"
                     /translation="MEVNAKAHHSQQLKQQFDNRLSRIEGQVKALRRMIDDDVYCDEV
                     LNQVTSVMAALNGIRLLLLKKHINSCVREQLMEGHTEVVDELMKTIGRMTKQ"
     gene            3098..>5337
                     /locus_tag="P5123_10920"
     CDS             3098..>5337
                     /locus_tag="P5123_10920"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR01511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0019829 - ATPase-coupled monoatomic cation
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal translocating P-type ATPase"
                     /protein_id="HRX16815.1"
                     /translation="MKNTFILSGMSCASCAEHIRKAAASVEGVNDASVNLASERLTVH
                     SKGGDKILSQIEQKIADAGYLAKRITDNRKVTISIGGMSCASCAKSIEGALSQMDGVT
                     LATVNFATERLTVEYNPEQVRLGMIKQRIIDNGYTPLESEKSDGEDEQKRKDRDIKVL
                     KIKFFIAALFTIPLFYLAMAPMITWFSVPFPKFLMPMTNPLYYALVQLGLALPVIVAG
                     HRFYSGGGKAIIRRSPNMDSLVAMGTSAAFLYSLFSVSRIVAGQISAVHHLYFETAGV
                     IITLVMLGKLMESSAKRRTSGAIKKLMRLAPQTATLIHNGKEVVVPVHEVEVGDVIVV
                     KPGEKIAVDGEITEGQTSIDESMLTGESLPVERGPGDTIYAATINGNGSICFRATKVG
                     DDTALSAIIRLVEEAQGSKAPVALLGDVISGYFVPAVFIIALVSSVAWLISGVPVTFA
                     LTIFISVLLIACPCALGLATPTAIMVGTGKGAEMGVLIKSGEALEVAHKIDSVVFDKT
                     GTITEGKPRVTDIAVADGGDQNLLLQIAASAEKNSEHPLGSAIVNEAKSRNLKLLKVA
                     GFSAVTGLGIEAVIDSKKVLLGNLKFMREKGIYVNELSDKSENFALEAKTPVYVAVDG
                     SLYGIIAVADTIKEGSREAVNRLIKLGMDVIMITGDNSATANAIANQVGIKNVLSEVL
                     PQDKAAAIEKLQKDGKIVAMVGDGINDAPALVRADVGIAIGSGTDVAIESADIVLIRG
                     DVRDVVA"
BASE COUNT         1587 a          994 c         1163 g         1593 t
ORIGIN      
        1 aagctatata gataatcaat ggttgatcct taacgttcta tgggcattta agtaaaaaat
       61 taagtagtct gtttttagaa aacaaatcag gtttaatttt acgatatgag cctgatttgt
      121 tgtgttttct aaacatattt tcattgacac taacattttt gtttctactg tacaatcaat
      181 gaaaacagaa ataattgata ctgaaaaaaa atctctgcta tctttaatga aagcaaatta
      241 caggctcaaa tatgttcagg aagaggatct tcttgaactt ttatcctatt gtcactttcg
      301 taaattcagc aaaggctcct atgtttttat ggaaggagat gatgtagaaa gtctttatat
      361 agtatcttcc ggcaagatcg agcttaatat gaataaccac aagtttcaag agaaaatatt
      421 tagcattata ggccccggtc aactgttagg tctatctgag atttttaact gtcatcaaat
      481 acatacaaca aatgctcttt gtgagcagga ttgtacggtc gctgccatat cacgtgatca
      541 tttccgttca attattaccg cgcttccatc tctctctttt gctgtttgca tcataatggg
      601 aaatatgatc ggagagttaa gacatgagct ttcactctca agtgccgaag tcaagataat
      661 gagctattta aaaaatctta tgattaaatc agcagtatta aaagatgggc agatcatagt
      721 tcctcgagaa gtcagatatg aaaagctggc aaaaattctt aatattacaa gagagactac
      781 aagccgtgtc ttcatctcgc ttaaagacag gcatattata gatatacata aggattacta
      841 tataattctt gatgaagaaa aaatcatgaa catagttcct cactactcct gcttgtcaca
      901 gagctacaac taattaccga tcatattttt cctcttgcat caaagcgcgg ataaggcgct
      961 aatcaattta tcgcatccac catttttaat gaattcagaa tgctgttcca cactatcaaa
     1021 gtgcttgccg gttattttat tgcacaacat ttcagagtcg gtttgagagt aaaaagcact
     1081 caatactttt tttgtaacag gattattgaa ataggaaggt gtttgtccgg aattgtctct
     1141 gcaccagaga agcatcttgt accagataac ggcgctcaat gctccacaag cataaccgct
     1201 aaggccgata ccgcctgcga aaccggctgc ggctgtggta tcttcttcag aagctcccat
     1261 ttttttcagc acctcggaag aacagcttac gcaagagggc ttgcactttg cctgattggt
     1321 caagccctca tcagcggata ctagggcttc cggtgtccac ctggcaatca gattgaaaca
     1381 tggactgaat acaaaaccac ggataatgac cttgaacatg taggtaaaaa actgccacct
     1441 gttttgccag tcggtgtttg taatatcctg gcagttaatc gaaccggctc ttttttgaaa
     1501 agattcaatt atattcttag aggcgtctat cgcggcttta attgctgtgt cgctgtttcc
     1561 gtatctctgg tacgattcaa taccacaagc cagagacgcg ccccaaagca ttccgcactg
     1621 ttggcctttc agggcaattc ctccagccag aattgcaaga gcctcctctt cggcatgcct
     1681 atcgttatta tactcacggt ttaaaagatg acacatcgcg gtagagcagg cgccgcgtct
     1741 ccagaatacc attcttggac tggcttgttt tgttttatcg cttttcatcg gttgtctcct
     1801 taggctgctt tttcttccca aatttagaat aaaagcaatt ttaatctttt tgatttactt
     1861 ccaccttaaa gctgaaaacc tgatctattt tcacatcaaa ggcgtttgcg attttgaaag
     1921 ccagctcaag cgagggtgaa tacttaccgg cttcaattgc tattattgtc tggcgtgaaa
     1981 cccctgtctt ctccgcaagc tgctgctggg tcatctcgtt tgcaaagaag cggtgttccc
     2041 tgatcttatt tgatattgta catttgtttt tcataatatg tttaccggct gtaatagtac
     2101 aggcgtgtaa aaccctccag aattgatccc agactaaaag agatgaatat aatattaagc
     2161 agatatgcag cggatccatc cataacgatc cagacaagag ccgaaataaa ccctattccg
     2221 gcaaaggtaa aaccaacacg taaagattta agctctatta tcttgtcacg ctcatcttcg
     2281 acgacctcca attctatcat cttttctata tctttgtcgt cacagtttcc agttttaatc
     2341 tgagaggcta tcgcgagaga gatcgaatat agaatatgaa aaagtatctg tatcacaatc
     2401 atggcgacaa tgccgattcc gataaatttg agcatagtaa ttgcctgctg ttttaaaata
     2461 gcatctgtat ctgttgctgc tgtcgagcag ccaaacgaat aatagcagta tgctgtcaga
     2521 actgctattc cggttactat cattataatg gtctttttct ctttgtaagc catgttggcc
     2581 tcctttacat aaagtatcac attgttgaca aaatgtataa aatactttac attatgtcaa
     2641 taataattgt caattttgta atattttctg ataactttat actgttagct ccttaaacct
     2701 tggttagatc tatgaaagat tagtctgtaa cattataata tcacttgacc cgtaatatta
     2761 tagaaatagg gtggtagggt atgctaccta atattttgaa ggagtttaaa atggaagtta
     2821 acgcaaaggc gcatcattca cagcagttga aacagcagtt tgataatcgt ctgtcacgta
     2881 tagagggaca ggttaaggct ttgcgtagaa tgatagatga tgatgtctat tgtgacgaag
     2941 ttcttaatca ggtgacatct gtaatggcag cgttaaacgg cataaggctt ttacttttaa
     3001 agaaacatat aaactcgtgt gtcagggagc agttaatgga aggacataca gaagttgtgg
     3061 atgaactaat gaaaacaata gggaggatga caaaacaatg aaaaatacat ttatattatc
     3121 tggaatgagc tgtgcttctt gtgcggagca tatcagaaaa gctgctgcat ctgttgaagg
     3181 tgtaaatgac gcgtcggtca atttagcttc agagcgactt actgttcatt cgaaaggtgg
     3241 tgataaaatt ttgtctcaga tagagcagaa gatagcagat gccggatatt tggcaaaacg
     3301 aattacggat aaccgtaaag ttacgatatc tatcggcgga atgagttgcg cgtcttgtgc
     3361 taaaagtata gaaggagcac tttctcaaat ggatggggta acacttgcaa ctgtaaattt
     3421 tgcaacagaa agacttactg ttgagtacaa tccagaacaa gtcagacttg gaatgataaa
     3481 gcagcgtatt attgataatg gatacactcc actcgaaagt gaaaaaagtg atggtgaaga
     3541 cgaacaaaaa agaaaagacc gagatatcaa agtgcttaaa ataaaatttt ttattgccgc
     3601 gctgtttaca ataccgctat tctatcttgc gatggctccc atgattacat ggttttcggt
     3661 tccgtttccg aagtttttaa tgcctatgac aaatccgctt tattatgccc ttgtgcaact
     3721 ggggctagcg cttccggtta ttgtggctgg gcaccgtttt tatagtgggg ggggaaaggc
     3781 aattattcgc aggtcaccta atatggattc tctggtggca atgggtacca gcgcggcttt
     3841 tttatacagc cttttttctg ttagccgcat tgtggccggt caaatcagtg ctgtgcatca
     3901 cctctatttt gaaacagcag gtgtaatcat aacccttgtt atgctgggta aacttatgga
     3961 atcttcggcc aagaggcgaa catcgggagc aataaaaaag cttatgcggc ttgctccgca
     4021 aacagctact cttatacata atgggaaaga ggtggttgtg cctgttcacg aggtagaagt
     4081 cggtgatgta atagtagtaa agccaggtga aaaaattgcg gtagacggtg aaataactga
     4141 aggtcagacc tcaattgacg agtcgatgct cacaggagag agtctccccg tcgaacgtgg
     4201 ccctggcgat accatctatg ctgcaacaat aaacggcaac ggtagcattt gttttagggc
     4261 tacaaaggta ggcgatgata cggcgctttc tgcgataata cggcttgtcg aagaagcaca
     4321 gggatcaaaa gctccggttg ctcttttggg tgatgtaatc tcaggttatt ttgtgcctgc
     4381 agtttttatt attgctcttg tttcatcagt agcttggtta atttcggggg tgcccgttac
     4441 atttgctctt actatattta tatccgtact tttaatagcg tgtccatgtg cgcttgggct
     4501 tgcgacgcct accgccataa tggtaggaac cggcaaagga gccgaaatgg gggtgcttat
     4561 taaatcagga gaggctcttg aggttgccca taaaatagat agtgtagttt ttgacaagac
     4621 cggaacaatt accgagggta agccacgcgt aactgatata gccgttgctg atggaggtga
     4681 tcagaatttg ctattgcaaa tagccgcgtc agcagaaaaa aattctgagc atcccttagg
     4741 tagtgcgata gtaaatgaag cgaaatcgcg aaatttaaag ttattgaaag tagcagggtt
     4801 ttcagctgtt acaggtctcg gtatcgaggc tgtaatagat agtaaaaagg tgctgctcgg
     4861 taatttgaaa tttatgcgag aaaaaggtat ctatgttaat gagctttccg ataagagtga
     4921 aaattttgca ctagaagcta aaacacctgt ctatgtggca gtggatggta gcctgtacgg
     4981 aataattgcg gtggccgaca caataaaaga aggtagccga gaagccgtaa acaggcttat
     5041 taaacttggt atggatgtta ttatgataac tggtgacaac agtgcgacag caaatgctat
     5101 tgctaatcag gtgggtataa aaaatgttct ctcagaagtg ctgccacagg ataaagcagc
     5161 agcaattgaa aaattgcaaa aagatggtaa aatagtagca atggttggtg atggtattaa
     5221 tgatgcgccc gcgcttgtac gcgccgatgt tggtattgct attggttcgg gaactgatgt
     5281 cgcaatcgaa tcggcagata ttgtgttaat aagaggagat gttcgagatg tggttgc
//
LOCUS       DAOTGZ010000188         5327 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_3064
            NODE_18327_length_5327_cov_6.039454, whole genome shotgun sequence.
ACCESSION   DAOTGZ010000188 DAOTGZ010000000
VERSION     DAOTGZ010000188.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868088
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 5327)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5327)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:22:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,785
            CDSs (total)                      :: 2,751
            Genes (coding)                    :: 2,732
            CDSs (with protein)               :: 2,732
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 12 of 19
            Pseudo Genes (internal stop)      :: 5 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5327
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_18327_length_5327_cov_6.039454"
                     /isolate="AMR_MDS_3064"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1301)
                     /locus_tag="P5123_10925"
     CDS             complement(<1..1301)
                     /locus_tag="P5123_10925"
                     /inference="COORDINATES: protein motif:HMM:NF012798.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HRX16816.1"
                     /translation="MGNIKNKTPVFLSINETNLLLKNTKNERHRIGISLMSYAGLRIS
                     ELTSLKVSDINLSKSFIKIVGKGNKERIIPLTPYLQTQLEKYLQRNSTRLVYDGLLVG
                     GCRSAWHRVVKKNSKKILGKGDVHCHTLRHSYATALYEKGVAIEKIAQLLGHNSIETT
                     MIYSHISLESKRKAVNILDQPKNRYKSKLFKFFNRSVEHQVSLNNVEGLVGRDDLVSE
                     INNQISKKRSFVLSGAQGCGKSALLRQIPDAIFLPEYLRKKSLVYILLLSSPGYLADS
                     EESESLKAELSKMKVDDLIDAVKSVGKIIVIDDISDCSKSDKKAIKEISKGPNVVVSA
                     SSKIADRKLFDTYVEVKPLKRHYTRHILSDMIHMVDHKQKEYVINDILHSAGDNLKEA
                     EYIARQLQFGKSTEEIVTPERSSNQVSIAPLLLLVLLFFFS"
     gene            complement(1456..1608)
                     /locus_tag="P5123_10930"
     CDS             complement(1456..1608)
                     /locus_tag="P5123_10930"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRX16817.1"
                     /translation="MKPLVLKAQAKELYDSLKVVKDINRDNLNDVNQKLLKMMLEVNE
                     MQNKFE"
     gene            1636..2199
                     /locus_tag="P5123_10935"
     CDS             1636..2199
                     /locus_tag="P5123_10935"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRX16818.1"
                     /translation="MIKSILLICSMSIFLSIFAQSSGFLDNEKAKEMSELDQKINKSL
                     ARIRATSESRAGDYLTLLPTVTVSRSAPTSDIQGSETYIGGSISLNGIFSISDASNKR
                     AKLKREGERKVKSLEYQITTLINRKYILKRKVWQKSKIKKALTNPLEIAKYDDDIDDL
                     NMRIEEISMKIEQQYAEIDYVCIEVEG"
     gene            complement(2312..2983)
                     /locus_tag="P5123_10940"
     CDS             complement(2312..2983)
                     /locus_tag="P5123_10940"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRX16819.1"
                     /translation="MSEESQISNELQEITNRIKSNVSRFEKLRREAGKLKQQIADDVL
                     FVAKNKNRLLPNRTLQDYIENDLKISKQYFYSIKRSYDFLIENNKAELIRDTDFRIVD
                     DISRVKDKEKQKELLAKTTFLTRDQIREEIQKYARHTSFDKKYNSKGSSAPEKTIASL
                     FEDLYTLIDYHPEQISKSKKEALSSLIGGEKTGGIIQRMMFTEKQLTAIRDHLDKLIK
                     RKKGY"
     gene            complement(3069..3719)
                     /locus_tag="P5123_10945"
     CDS             complement(3069..3719)
                     /locus_tag="P5123_10945"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRX16820.1"
                     /translation="MSSVKDKLKKNAKNKLAVTAKKPVVDNKVSVLNDIDKITFEIKS
                     NLDEIREIQEWWSEKKNRVVELKKSIMVRLIFVRDNKKNLLGSRTFEDYLVNEVGVSK
                     GYFYDYVRALGIATSYKKPNLLDSTDPSVILLADKIEDDDVKKAVINRAAELSREDVK
                     QIQKSSDLSPDYVSNYVPIANRTCNVKIGKDSIYIKSSEPDVLEKIKKFLSDNNYV"
     gene            complement(3716..4453)
                     /locus_tag="P5123_10950"
     CDS             complement(3716..4453)
                     /locus_tag="P5123_10950"
                     /inference="COORDINATES: protein motif:HMM:NF013793.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ParA family protein"
                     /protein_id="HRX16821.1"
                     /translation="MKTIVFASKKGGVSKTTSAIITALLLSKKAPTLLIDLDSQNALT
                     SFFFEDISSIEGRTILEAIKGEQLFSKVIQKVSDNLDIIPSVLEFEEIDDWQRTGKEL
                     ILKKELNKISEKYTYVVIDTPPSLRTETVLGLVAADVIIIPARLEKMDTRAIDFTIDK
                     TVNQIKEDFNPTLQNIYILPTQYNYQNRTVNDLALESLQEDYSDMVLDIKIQNSSKIS
                     QFLYLGYQQKLNEKDVPEYIELVELIK"
     gene            complement(4450..4617)
                     /locus_tag="P5123_10955"
     CDS             complement(4450..4617)
                     /locus_tag="P5123_10955"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRX16822.1"
                     /translation="MANAIISVKDDKSEYIIKEAKKKCIDEDINFTNATLALLEMWVK
                     GEISIKKGETV"
     gene            4762..5142
                     /locus_tag="P5123_10960"
     CDS             4762..5142
                     /locus_tag="P5123_10960"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRX16823.1"
                     /translation="MNLNQAIFNKINDLGITEKLKEHYYLKMECLGLMDLHVDKLRHE
                     NGTTLISLAHNGIQNGDVMADPDMEVVIDENIQEAYANRFQNDYVGIVTVNNTPENER
                     SMNEFLVTWLDNIKKSNYQFTKIE"
BASE COUNT         1810 a         1027 c          819 g         1671 t
ORIGIN      
        1 tacgagaaaa agaacaacaa caccagtaat aatagcggag caatcgaaac ctgattagaa
       61 gacctctcag gagtcactat ctcctccgta ctcttaccaa attgcaactg acgagcaata
      121 tattccgcct ccttaaggtt atcaccagca gagtgcaaaa tatcatttat aacgtactcc
      181 ttctgtttgt gatccaccat atgtatcata tcagaaagaa tatgtcttgt atagtgcctc
      241 tttaacggtt taacctcaac ataagtatca aacaacttac gatcagcaat cttggacgaa
      301 gccgaaacaa caacattcgg acctttagaa atctctttaa ttgctttctt atcagatttg
      361 gaacaatcag aaatatcatc aataacaata atcttgccaa cagacttaac agcatcaatc
      421 agatcatcta ccttcatttt agataattca gcctttaacg attcggactc ttcactatca
      481 gccaaataac ctggcgatga taataacaga atataaacca gactcttttt acgtaaatac
      541 tccggtaaaa atatagcatc cggaatctgg cgtaaaaggg cagacttacc gcaaccctgt
      601 gccccggata aaacaaaaga ccgcttttta ctaatctgat tatttatttc agaaacaaga
      661 tcatctcgac caacaagacc ctcaacatta ttcaatgaaa cctgatgttc aacagacctg
      721 ttaaaaaact taaacagctt agacttatac ctattcttcg gctgatccag aatattaaca
      781 gctttccgct tagactctaa agatatatgt gaatagatca ttgtagtctc gatactgtta
      841 tgccctaaca actgggcaat cttttcaata gcaacaccct tttcatataa tgcagtagca
      901 taactatgcc ttaatgtatg acaatgcaca tcgcccttac ccagaatctt tttagagttt
      961 ttcttcacca ccctatgcca tgccgacctg cagccaccaa caagcaaacc atcatataca
     1021 agccttgtac tgtttcgctg cagatacttt tccaactgag tctgcaaata aggagtcaga
     1081 gggattattc tttccttgtt acccttaccc actattttta taaacgattt agatagatta
     1141 atatccgata ctttaagaga ggttagttca gagatccgaa gaccggcgta ggacatcaaa
     1201 gaaatgccaa ttctatggcg ttcatttttc gtatttttga gcagtaaatt agtctcattt
     1261 atactcaaaa acacaggagt tttattctta atattaccca tataccacac taatacatga
     1321 catactatat accatattac ccgcgatttt caacaaaata tgtattttgt tgatactgat
     1381 actcaacaaa aacaggctaa aaagacaaaa aaaacggctc ataaacagaa ccgattttgt
     1441 gtaattttag tgaatttatt cgaatttatt ttgcatttca ttaacttcga gcatcatttt
     1501 aagcagtttt tgatttacat cattaagatt atccctatta atatccttaa cgaccttcaa
     1561 agaatcatac aactctttcg cctgcgcttt taaaaccaac ggtttcataa aatctcctaa
     1621 agaaacgagg ttcacatgat taaaagtatt ctattaattt gtagtatgtc aattttttta
     1681 tcaatttttg ctcaaagttc tgggttttta gacaatgaaa aggcaaaaga aatgagcgaa
     1741 ctggaccaga aaatcaataa gtcattagca cgaattagag caacatcaga atcacgcgcc
     1801 ggagactatt taacactatt accaaccgta acagtatcaa gaagcgcgcc aacatcagac
     1861 attcagggtt cagaaacata tataggtgga tcgatcagtt taaacggaat attctcaata
     1921 agtgacgcat caaataaacg cgcaaaatta aaaagggaag gggaacgcaa ggtaaaatcc
     1981 ctggaatacc agatcacaac attaattaac cggaaataca tactaaagcg taaggtttgg
     2041 caaaagagta aaattaagaa agcattaaca aatcccttag aaattgcaaa atatgatgat
     2101 gatatcgatg acttgaacat gagaattgaa gaaatatcca tgaaaataga gcagcagtat
     2161 gcagagatcg attatgtatg tatagaagtc gagggataat aaaagcctga cggacgaacg
     2221 tcaggcttta tgatacaaaa ttgatgcaac tgataatcat taatatactc gtctgtatgg
     2281 aaaattactt aagcattttt tacagaataa atcaatatcc cttctttcgc ttaataagct
     2341 tatccaaatg atctcttatc gctgttagct gtttttcagt aaacatcata cgttggataa
     2401 taccacctgt tttttcccca ccaataagac ttgataatgc ctctttttta gatttgctaa
     2461 tctgttctgg gtggtaatca ataagagtat aaagatcttc aaaaagggag gctatggttt
     2521 tttcaggagc agaagaacct tttgagttat attttttgtc aaaagaagta tgccgggcat
     2581 acttttggat ttcttccctt atttgatctc gagtaagaaa agttgtttta gccaaaagct
     2641 ctttctgttt ttctttatct ttaacacgcg aaatgtcatc aactattctg aaatctgtat
     2701 cacgaatcaa ctctgctttg ttattctcta ttaaaaaatc ataactacgt tttatggaat
     2761 aaaaatactg cttagatatt ttaaggtcat tttcaatata gtcttgaaga gtgcgatttg
     2821 gaagaagtct gtttttattt tttgcaacaa acaaaacatc atcagcaatc tgttgtttaa
     2881 gtttgccggc ttccctacga agtttttcaa accgagatac attactcttt attcgattag
     2941 taatttcttg taattcatta gatatttggc tttcttcaga cataatcaaa actccacaga
     3001 tttactgact aaaaacattt agaaaataaa gaatcaaaaa gtacggcaag gggtgctcgc
     3061 cgtacttttt acacataatt attatcagaa aggaatttct taattttctc taaaacatca
     3121 ggctcagacg actttatata aatactgtct ttacctattt ttacattaca ggtacggtta
     3181 gcaataggaa cataattcga aacatagtcc ggtgataaat cactggattt ttgtatctgt
     3241 tttacatctt cgcggcttaa ttctgcagct cggttaataa cagccttttt tacatcatca
     3301 tcttcgatct tgtctgccag caatatcacc gaaggatctg tactatcgag aagattcggc
     3361 tttttataag aagtagcgat acctaaagca cgtacataat cataaaaata acccttagaa
     3421 acccctactt catttaccaa ataatcttca aaggtacgac ttcccagcag attttttttg
     3481 ttatctctaa cgaaaataag gcgtaccata atgctctttt ttaactcaac aacgcgattc
     3541 ttcttttccg accaccactc ctgaatttct ctgatttcat ccagatttga tttgatttca
     3601 aaagtaattt tatctatatc gttaagaacg gaaaccttgt tatcgacaac cggtttttta
     3661 gctgtaacag caagcttgtt ctttgcgttt tttttcagtt tatctttaac actgctcatt
     3721 taattaactc cactaattca atatattccg gcacatcttt ttcattaagt ttttgctgat
     3781 aaccaagata caaaaactga ctgattttcg aagaattttg aatcttaata tcaagcacca
     3841 tatcactata gtcttcttgc aaagactcaa gagccaaatc attaaccgtc cggttttgat
     3901 agttgtactg agtaggcaaa atatagatat tttgtagggt agggttaaag tcttctttga
     3961 tctggttaac tgttttatca atggtaaaat cgatcgcgcg agtatccatt ttttcaagac
     4021 gtgccgggat aataatcaca tcagcagcaa caagacccaa aacagtttca gttcgtaaac
     4081 tcggaggcgt atcgataaca acataagtat atttctctga gattttatta agctcttttt
     4141 ttagaataag ctctttacca gtcctctgcc agtcatcaat ttcttcaaac tcaaggaccg
     4201 atggaataat atccagatta tccgaaacct tttgaatcac cttagaaaat aactgctcac
     4261 ccttaatcgc ttcaagaatt gtacgacctt caatagagct aatatcctca aaaaagaaag
     4321 aggtcaaagc attttgagag tccaggtcaa tcagtagagt aggggctttt tttgaaagta
     4381 aaagagccgt aataatcgcg ctggtggttt tactgacacc gcctttttta gaagcaaaaa
     4441 ctattgtttt catactgtct cccctttctt gattgatatt tcacctttga cccacatctc
     4501 aagaagtgct aacgtcgcgt ttgtgaaatt gatatcttca tcaatacatt tcttttttgc
     4561 ttctttgatt atgtattctg atttgtcatc tttaacacta ataatcgcgt ttgccatttt
     4621 ttcacctttt caatatatca ttatatcatt aaatatatat ttatatattt gtcaatatat
     4681 tcttgacatt atcacataac actatacatt tgtatagtgt ttaaaaactc taataaccca
     4741 aattaaagga ggttaaaact tatgaatttg aaccaggcaa tatttaacaa aattaatgat
     4801 ctggggatca ccgaaaagct taaagagcac tattatttaa aaatggaatg tttaggatta
     4861 atggatctgc atgttgacaa acttcgccat gaaaatggaa caacattgat ttcacttgct
     4921 cacaacggaa tacaaaacgg agatgtcatg gcagatccag acatggaagt agtaatcgat
     4981 gagaacatac aagaagccta cgcaaacaga tttcaaaatg actatgtagg aattgtaact
     5041 gtaaataata ccccggaaaa tgaacgcagt atgaatgaat ttttggtcac atggttagac
     5101 aacataaaaa aatcaaatta tcaatttact aaaatcgaat aatcaaagcg gtcattacga
     5161 ccgctctaaa atataacgta aataccagca aaatcggtat taaaatcgcc ggtcccagca
     5221 aaagcgaaaa tggcgcgcac tacagtgaag aactataaat atttaagagg ataaataata
     5281 ctggagccca ccaaaaaagg cccccacaga agatgggctt tttttgg
//
LOCUS       DAOTGZ010000189         5317 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_3064
            NODE_18373_length_5317_cov_4.755796, whole genome shotgun sequence.
ACCESSION   DAOTGZ010000189 DAOTGZ010000000
VERSION     DAOTGZ010000189.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868088
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 5317)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5317)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:22:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,785
            CDSs (total)                      :: 2,751
            Genes (coding)                    :: 2,732
            CDSs (with protein)               :: 2,732
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 12 of 19
            Pseudo Genes (internal stop)      :: 5 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5317
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_18373_length_5317_cov_4.755796"
                     /isolate="AMR_MDS_3064"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(19..2859)
                     /locus_tag="P5123_10965"
     CDS             complement(19..2859)
                     /locus_tag="P5123_10965"
                     /inference="COORDINATES: protein motif:HMM:NF021492.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HRX16824.1"
                     /translation="MKRIALIMLIILLGGFISAQQSSSMLYDQALEAIRAKNYKSAEL
                     ILQSIIKNDDGYKERATFNLAKSIHYQKRYKSAVFEFNRYLSISTDDNLKTEARYWIA
                     RSHYLDRNYHHAIEEFKRFISENPNDNYLITDSYRQIGYIYFRQRRYDEALIEWRNAL
                     QVAEDDALADKIRYDIALAYFNTISYNESEKILKTLLSSSKQVDVKTRANILFGRIEQ
                     RRGHHKQALVYFGRVTKELLPRRPYYNVLYYMGTSFIELKDDRRAIAALKKYLSYSSG
                     NDSSLNDKASLDLGRILSRANREDEALEILLELIEKSENQILKVEAINIAGRIYLKRN
                     DLYKARPLAEKILLYEIDYDSRNAYLLLSDIYVQLAMFSRAEKILLSLQKELAFDPEL
                     DMIQYKLSLVYLREKKYDKAIEGFKRIDDFNPFSSYKNEYHYFMAVSSYDKKNYSETI
                     KYCTIYLKKPEILNAYDARILRLDSNMRLKRFDSARLDANFLITNFPKGAKNSGVIYD
                     YALFLESNKRDNTKYINYVVTSFPKSDITFIILRIQGEKHFNNAEYKKAEEVYARLFS
                     AKEKNADIEIVSRYLDILYKNRNYKLIDQITGNAENADERVRPIMVLYAARSAFKRNH
                     FQTSSSLYNGLYISDSIKMLADQDYYYMAKNEIYLDNLEAAKSFTKKIGESNYREKAT
                     RLISDFLIRKNGVEDAAVFAEDVFKVTEKPGLKSEIALIAAENYLLSGNSDKSFKILS
                     TLKKHNHKTVNLMVNILIGKGDYNHLFEFLNKTRDVFSLPDDKSFLLLLGKLSDITLN
                     YKQDSLFLFYNNILMSNKDYRGKAESNFAYFYYINDNANKAFSYSMSALNKNPTDPRS
                     LFIAAEIDYYHYKNYRRSKQLFDRLLLLPYDRGMFVAKGRISYALILFSQKKYSDAIA
                     HLKHLADHYRGGALARASAIIKSHYGYKNETD"
     gene            complement(2861..4540)
                     /gene="argS"
                     /locus_tag="P5123_10970"
     CDS             complement(2861..4540)
                     /gene="argS"
                     /locus_tag="P5123_10970"
                     /EC_number="6.1.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020770766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004814 - arginine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006420 - arginyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arginine--tRNA ligase"
                     /protein_id="HRX16825.1"
                     /translation="MKRIKIVIGELVSDALVSAVKDGKFGDCNASDLPDVKIEYPKEE
                     KFGDYSTPIALESARILKKSPFDIGKILSEYLSNSDLIEKIDVVKPGFINIFVSRNYL
                     VKSVVQAVEQGESYGKSEKQNPLRYSLEFVSANPTGPLNVVSARAAALGDSLANIIEA
                     SGDHVDREFYVNDHGNQVNLFGLSVYSRYREILGEKIDFPDDGYQGEYVKDIAQHVFD
                     NKEFNADEFASEEEIISFFSKYAISFVVKSQQTVMEKFRVKYKQWFRESTLHENGIVN
                     QTFDEMVSRGVIYDEEGKKFFKATDYDDDKDRVVVREDGRPTYLMADIAYHENKYTRG
                     YDSIIDIWGPDHHGYIARLRGAMIALGHDSEKFRVLIAQQVNLIMDGEAVKMSKRLGR
                     FSTMQELIDDIGVDAARYFFVARSMDSHLDFDLELAKRRTSENPVFYLQYAHARICSI
                     FREAEKRDINIDACSLNQDHLDNPEALSLLKMIVRFPEEIADAASVFEVHRLANYLLR
                     LAQDFHRFYTVNRILTDDTERTESMLIICTAVKNVIRNGLHILGVSAPDSM"
     gene            4942..>5317
                     /locus_tag="P5123_10975"
     CDS             4942..>5317
                     /locus_tag="P5123_10975"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRX16826.1"
                     /translation="MINKRDTIITFVIFVTGTFILAWNIGHESFWADEAFTGVMIRHS
                     FADIYEFCKMEMHSAFYYWMLRIFSLIFGSGEISLRVFSLLGTVALAGLGPTAIKRLF
                     NPIAGWMFSAAVFLAPGLVAYSQ"
BASE COUNT         1677 a          997 c          838 g         1805 t
ORIGIN      
        1 ataatatctc gaagacgatt aatctgtttc atttttatat ccatagtgag atttaattat
       61 agcagacgcc ctggctaaag ctccacctct ataatggtcc gcaaggtgtt tcaaatgtgc
      121 tatggcatca gagtattttt tttggctaaa aagaattaat gcatagctaa tgcgtccctt
      181 agcaacgaac attccacgat cataaggaag taaaagtaat ctgtcaaata actgtttcga
      241 tcgtctataa tttttatagt gataataatc tatttcagcc gctataaaaa gcgatctggg
      301 atccgttgga tttttattca gtgcagacat gctgtaactg aatgctttgt ttgcattgtc
      361 attgatataa taaaaatagg caaaatttga ttcagctttt cctctgtaat ctttattaga
      421 cataagtata ttattataaa acaaaaaaag tgagtcctgt ttgtaattca aagttatatc
      481 cgataatttg ccaagtaata ataaaaacga tttatcatca ggtagtgaaa aaacatcccg
      541 agttttattt aaaaactcaa aaaggtggtt gtaatcgccc tttccgatta aaatattaac
      601 cataagattt actgtcttat gattatgttt ttttaatgta gacaatattt tgaaactttt
      661 gtcactattg cccgaaagca aataattctc agcagcaata agagcgattt cgcttttcaa
      721 tccaggtttt tctgtgactt taaatacatc ttcagcaaat acagcagcgt cctctactcc
      781 attttttctt attaaaaaat cagaaataag tcgggtagct ttttcgcgat aattggattc
      841 acctattttt ttcgtaaaac tttttgcagc ttcaaggtta tcaagataga tttcattttt
      901 agccatgtaa tagtaatctt gatcggctag cattttgata gaatcagata tatatagacc
      961 gttgtaaagc gaagatgatg tctggaaatg attacgttta aatgccgagc gagccgcata
     1021 taaaaccatt attggacgaa ctctttcatc cgcattttca gcgtttcctg taatctgatc
     1081 aattaattta taattacggt ttttatataa aatatccaga tatcgtgaaa caatttctat
     1141 atcagcattt ttttcttttg cactaaataa tctcgcgtat acttcttccg cttttttata
     1201 ttctgcatta ttgaaatgtt tttctccctg aatacgtaga atgataaaag taatatccga
     1261 ttttgggaaa cttgtcacta cataatttat atattttgta ttgtctcttt tgtttgattc
     1321 caaaaataac gcgtaatcat aaattacccc tgaatttttt gcacctttag gaaaatttgt
     1381 tatgagaaag tttgcatcta atctagcact atcgaaccgc ttaaggcgca tgtttgaatc
     1441 aagccttaat attcttgcgt cataagcgtt taatatttca ggttttttta ggtatattgt
     1501 acaatatttt atggtctctg aataattttt tttgtcatag ctagaaacgg ccataaagta
     1561 atgatactca tttttataag aagaaaatgg attaaaatca tcaatacgtt taaaaccctc
     1621 aatagcttta tcatattttt tttcacgaag ataaacaagt gagagtttat attgaatcat
     1681 atcgagttca ggatcgaaag ctagttcctt ttgaagagac agaagtattt tttcggcacg
     1741 actaaacatt gccaattgaa cataaatatc ggacaaaaga agataagcat tgcgcgaatc
     1801 ataatcaatt tcataaagaa gaatcttctc ggcaagaggt cgggctttat acaggtcatt
     1861 acgtttaaga taaatacgcc cggcaatatt aatcgcttca acttttagaa tctgattctc
     1921 ggatttttca attaactcaa gaagtatttc aagtgcctca tcctctctgt tcgctcgtga
     1981 taatattcta cctaaatcca gactcgcttt atcattaaga gaagaatcat taccgctaga
     2041 ataactcaaa tattttttta atgctgctat agctcgccta tcgtctttta attctataaa
     2101 agatgttccc atgtaataca gaacattata gtaaggtcgt cttggcaaaa gctctttagt
     2161 tactctgcca aaatatacta acgcctgctt atgatgtccg cgccgctgtt caattctacc
     2221 aaacaaaatg tttgctcttg tttttacgtc aacctgctta gatgaggaca acagagtttt
     2281 taaaattttt tccgattcat tataactaat agtattaaaa tatgccagcg caatatcata
     2341 ccgaatttta tcagcaagtg cgtcatcctc agcaacctgc aaggcgtttc tccattcgat
     2401 taatgcctca tcatatctgc gttgtctaaa atatatgtat cctatttgac gataactatc
     2461 tgtaatcaaa taattatcgt tagggttttc tgaaataaaa cgtttaaatt cttcaatggc
     2521 atgatgataa ttgcgatcaa gataatgtga ccgtgcaatc cagtatcgtg cttctgtctt
     2581 taaattatca tctgtactta ttgaaagata acgattgaat tcgaatacag cagacttgta
     2641 acgcttttga taatgtatgc tctttgcaag attaaaagtt gcgcgttctt tatatccatc
     2701 atcattttta attatggatt gtagtattaa ctcagcagac ttataatttt ttgcacgaat
     2761 tgcttccaga gcctgatcat agagcattga actactctgt tgagcagaaa taaatccccc
     2821 gagcagaata atcagcatta ttaaagcaat tcttttcatt ttacattgag tccggagcac
     2881 taacacccag aatatgcaaa ccattacgaa taacattctt aacagctgta caaataatca
     2941 acattgattc tgtgcgctct gtgtcgtcgg ttaaaattct gttaacagta taaaatctgt
     3001 gaaaatcctg agccaatctt agaagataat ttgcaagacg atgtacttca aaaaccgaag
     3061 cggcatccgc gatttcttct gggaaacgaa caatcatttt tagaagagaa agagcctccg
     3121 gattatccaa gtgatcttga tttaaggaac acgcatcaat attgatatca cgtttttccg
     3181 cctctctgaa tatcgagcaa attcttgcat gagcatactg taaataaaaa acagggtttt
     3241 cacttgttcg tctttttgct aactcaagat caaaatcaag gtggctatcc atggatcgcg
     3301 caacgaaaaa atatcttgcg gcatcaacac ctatatcgtc aatcagctcc tgcatggtag
     3361 aaaatcgccc caaccttttt gacattttaa cggcttcgcc atccattata agattaacct
     3421 gctgagcaat taaaacccta aatttttcac tatcatggcc aagagctatc atcgcgcctc
     3481 ttaatcgggc aatatagcca tggtgatccg gtccccaaat atcgataata gaatcataac
     3541 cacgtgtata tttattttca tgataagcaa tgtcggccat tagataagtt gggcgaccat
     3601 cttctcgaac aacaacacga tccttgtcat catcgtaatc agtagcctta aaaaactttt
     3661 tcccttcttc gtcgtatata acgccccgag agaccatttc atcaaaagtc tgattaacga
     3721 taccgttctc gtgtagagtg ctctcacgaa accactgctt atattttaca cgaaactttt
     3781 ccataacggt ttgctggctt ttaaccacaa aagagattgc atatttacta aaaaaagata
     3841 ttatctcttc ttctgatgca aattcatctg cattgaactc tttattgtca aaaacgtgtt
     3901 gagcaatgtc ttttacgtat tctccctgat agccgtcatc cgggaaatca attttttcgc
     3961 ccaagatttc acgataacgg ctgtaaaccg aaagcccaaa tagattcacc tgatttccat
     4021 gatcgtttac gtaaaattct cgatctacat gatcgcccga tgcttcaata atatttgcaa
     4081 gtgagtcacc aagagcagca gcgcgagccg aaacaacatt aagaggacca gtcggatttg
     4141 ccgatacgaa ttcaagactg taacgaagtg gattttgttt ttctgatttt ccgtatgatt
     4201 caccctgttc aacagcctgt acaacagatt ttaccaaata attacgagac acaaaaatat
     4261 taatgaaacc aggctttaca acatctattt tttctattaa atccgaattt gacaagtatt
     4321 cagagagtat tttacctata tcaaacggtg attttttaag aatacgagca ctttcaagag
     4381 caattggcgt ggaataatcg ccaaatttct cttcttttgg gtactctatt ttgacatccg
     4441 gcaaatcaga agcattgcaa tcaccaaatt ttccgtcttt aacggcactc acaagagcat
     4501 ctgaaactag ttctccaata acaattttta tacgtttcaa tatattaccc cgattctcat
     4561 cctgatattt tgcagttttg acataaacat gatataaata acttacacac atgaattcaa
     4621 ttgatacaaa actactattt aggacactat gatgaagcgt ctaaaaaaga caactttttt
     4681 ctataagccc agcttgccgt taacttaaat tattaaacta aaaaaactct ctcaattctt
     4741 tatttgtttt ccagtcaata cctactattc ttcccaatta ttcaaatttt gtattttttc
     4801 gtgataatgc gaaaattata gtctctttta gtactaaagt tgtatgaatt gtaactactt
     4861 ttttacagta atttctttac ataaattata gatagattat ttatgaataa aaaagcctct
     4921 tgggctcata aggatgtaat catgataaac aaaagagata caattataac ttttgttata
     4981 tttgtaacgg gaacctttat tttggcttgg aatattgggc acgaaagttt ctgggcagat
     5041 gaagccttta ccggtgtaat gattcggcat agttttgctg atatctatga attttgcaaa
     5101 atggaaatgc actctgcatt ctattactgg atgctcagaa ttttttcact gattttcgga
     5161 agcggagaga tatctctcag agttttttca cttttaggta ccgtagctct tgccggttta
     5221 ggaccgactg ctatcaaacg tcttttcaat cctatcgctg gatggatgtt ttcagcagca
     5281 gtttttcttg caccaggtct ggttgcgtat tcacagg
//
LOCUS       DAOTGZ010000190         5311 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_3064
            NODE_18403_length_5311_cov_4.321918, whole genome shotgun sequence.
ACCESSION   DAOTGZ010000190 DAOTGZ010000000
VERSION     DAOTGZ010000190.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868088
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 5311)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5311)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:22:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,785
            CDSs (total)                      :: 2,751
            Genes (coding)                    :: 2,732
            CDSs (with protein)               :: 2,732
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 12 of 19
            Pseudo Genes (internal stop)      :: 5 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5311
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_18403_length_5311_cov_4.321918"
                     /isolate="AMR_MDS_3064"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..262
                     /locus_tag="P5123_10980"
     CDS             <1..262
                     /locus_tag="P5123_10980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010417783.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HRX16827.1"
                     /translation="LTTVTRQISEKGEDIVMSTAMKLREEGKLEGKLEGRLEGKLEGR
                     LEGRLEGKLETAQKMLLKGFSVQEITEITGISEKEIASLKKM"
     gene            521..964
                     /locus_tag="P5123_10985"
     CDS             521..964
                     /locus_tag="P5123_10985"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRX16828.1"
                     /translation="MNVDDACKVKLASKPSNLTVKMMANNDVAEIELPDQSFSVGYSE
                     YEFNIVLPSEYISIYVENNSSYSINKFWYANFTCPENDEWTGSIEYDNDIDPGYWLWI
                     GFFKRENIKAVAFTHTSDHSYDWKYDFFETDPDEYGAYVHLFRLN"
     gene            complement(979..2076)
                     /locus_tag="P5123_10990"
     CDS             complement(979..2076)
                     /locus_tag="P5123_10990"
                     /inference="COORDINATES: protein motif:HMM:NF012568.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyprenyl synthetase family protein"
                     /protein_id="HRX16829.1"
                     /translation="MKTKSPDYIAAIREYLDKYALQIKERYSVYGQAATNEISRYILR
                     EGKYIRPRLFLLTAQLYGADYTPALVQCACSLELFHLFALAHDDIIDGDTTRGSLASL
                     GCGGKYSNNPKTENRILLGDILYSLAFETITRADISPDIKIELQKLMIETSVITAFGV
                     LKEPLLSGENFDTIDKLFRLYWQKTGMYTFASPMAGGYLTRHENYKRSSKNSNGINNK
                     NSKANSRLENREIKLLKELGFLWGCAYQLQDDFNDCRNIKHANLLDLPAEQAIGNSSH
                     KTKKMTESTASNANPNDKLLEELFSNSNTDKEKYGTFENYVTKNITLLLDKARLTAEK
                     LDLPANNKQKLIEFAELFFYKKRAVVERQPF"
     gene            complement(2066..2965)
                     /locus_tag="P5123_10995"
     CDS             complement(2066..2965)
                     /locus_tag="P5123_10995"
                     /inference="COORDINATES: protein motif:HMM:NF013226.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prenyltransferase"
                     /protein_id="HRX16830.1"
                     /translation="MIKELSLYLSSLRAYSFPLSISSALIGPAWAYSSGHRIDTVRLF
                     LVCATVLLFHSAVNMLNDYYDYNKGVDKNERQLAIQRNAPRFLLHYALLLFFIGASFG
                     AILVYLSGLPLLIMGTTGAVLSFFYTGKKLSLKYMALGELTVFITFGPLLGLASLYAV
                     SRTFSLHAALLASAPGLLAALVLFLNNLRDIENDKKAHIKTLPMYFAKKTSTLVALSA
                     LMLPYIIIVCGTISGFFTLYLLLSLLSLPAAIPVIKEIFSNNGTPDYDKALMGTIRIH
                     TLFTVLCITGLVASVFFGRLFEN"
     gene            3363..5135
                     /locus_tag="P5123_11000"
     CDS             3363..5135
                     /locus_tag="P5123_11000"
                     /inference="COORDINATES: protein motif:HMM:NF012869.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HRX16831.1"
                     /translation="MLKILRTFAEYSRPYRGAFILANTCMVIMDLTSYLLPVLIKYAT
                     DTVFDRVTKTGDLKILYIVGSAIIISGVVRGLLSHFMIRSYWYVGESVVKDIRNSLYE
                     KLQHLGASFYAKARTGDLMSRLTTDIQLIRNFYAFGVEHRLRIVLISVTILLIMLYQN
                     WRLALVVYIFLPIIYFVIVYFSSKLQKAVMRKHESAGALNSTLQENLTGIRIVKAFAV
                     EDEQKKIFEKSNLRLFNADLVVTTLQAHLNPLLQLTGGAGTLVILVYGGYQVIQGSMT
                     LGTLIGFMTYLMIIRFPLFILAFNTSLVNLAEGASKRIKEILILEDQREGDNGTVTNK
                     IKGKLEFKNVTFGYGERKPVLRDLSFSIREGERVALFGLNGSGKSSLISLIPRFYCPD
                     SGKILLDDTPLPEWSLEALRLQIGTVLQETFLFSMTIGENIAFGRPDASLQQIKEAAA
                     SAQIDTFIESLPEGYDTYVGESGSGLSGGQRQRIAIARALLQNPRILILDDCTSSLDP
                     QTERNIQNELRNLMSGRTTLIIAQRISTLRLADRIIVLDRGMIQDFDSHENLLKKNEL
                     YKATYETQMVCSESIIEDSLSMRS"
BASE COUNT         1573 a          941 c         1156 g         1641 t
ORIGIN      
        1 cttgacgact gtaactcgac aaatatcgga aaagggtgaa gatattgtta tgtcaactgc
       61 gatgaaatta agagaagagg gtaagcttga gggtaaactt gaaggcagac ttgagggcaa
      121 acttgagggt aggcttgagg gtagactgga gggtaagctc gaaacagctc aaaaaatgct
      181 tttgaaaggt ttttctgtac aggagattac cgagattacc ggtatttcag aaaaagaaat
      241 agcttcttta aaaaagatgt gatgaagtca atgataaata atcagacaat aatcgatctg
      301 atttttgatt gctttgtaaa caggatgtcc atgaccaaaa ctcttttcta cagaatataa
      361 gaatcataaa cgtgctattg ttgataagaa agcgataaag cgatactgtt atagtaaagg
      421 gattgaagat ccaatcgagg atacttatga agctacattt tataaccgta caggtggact
      481 gcttgataat gttactgtaa atggtttttc ttgtggtgaa atgaatgttg atgatgcatg
      541 taaggtcaaa ttagcatcta agccttcaaa tttaactgta aaaatgatgg caaacaatga
      601 cgttgctgaa attgaattac ctgaccaatc ttttagtgtc ggctatagtg aatatgaatt
      661 taatattgtt ttaccttctg agtatatatc tatttatgta gaaaataact caagctatag
      721 tataaataaa ttttggtatg ctaactttac ttgtcctgaa aatgatgagt ggactggatc
      781 gattgaatat gataatgaca ttgacccagg ttattggctt tggattgggt ttttcaaaag
      841 agaaaatatt aaagctgttg catttactca tacatctgac catagttacg attggaaata
      901 tgattttttt gaaactgatc ctgacgagta tggggcgtat gttcatctgt ttcgtttgaa
      961 ttaacacttt tctttatatt aaaaaggctg tcgctcaacg acagctcttt ttttataaaa
     1021 aaataattcc gcaaactcta tcagcttttg cttgttgttt gctggaaggt caagtttctc
     1081 tgctgtaagg cgtgccttat ctaaaagcag ggtgatgttt ttggtcacat aattttcgaa
     1141 tgttccatac ttctctttgt ctgtgttgct gttgctgaaa agttcttcta aaagcttatc
     1201 gttggggttt gcatttgaag cagtactttc agtcattttt tttgtcttgt ggctgctatt
     1261 accgattgct tgttctgccg gtaaatctaa aagattagca tgtttaatat tgcggcaatc
     1321 gttaaaatca tcctgcagtt gataggcaca accccataaa aatcccagct cttttagcag
     1381 tttaatttct ctgttttcaa gtctgctatt tgctttagag tttttattgt taataccatt
     1441 actatttttg ctgcttcttt tgtagttttc gtgtcgagta aggtatccgc cagccattgg
     1501 tgacgcgaat gtgtacatac ctgtcttctg ccaatatagc ctgaaaagtt tatcaatagt
     1561 gtcaaagttt tcgccgctta atagcggttc tttgagtacg ccaaatgccg taataacaga
     1621 tgtttcgatc ataagttttt gtagctctat ttttatatca ggtgaaatat ctgctctggt
     1681 gatggtttcg aaagcaaggc tatagagaat atcaccaagc agtatcctgt tttcggtttt
     1741 ggggttattg ctgtatttgc ctccgcatcc aagacttgca agcgaaccgc gtgttgtgtc
     1801 tccgtcgata atatcgtcat gcgcgagtgc aaacagatgg aaaagttcaa gcgaacatgc
     1861 gcattgaaca agagctggtg tgtagtcggc gccgtaaagt tgggctgtta aaagaaaaag
     1921 acgcggtcgt atgtattttc cttctcttag aatataacgg gaaatttcgt ttgtggcggc
     1981 ttgtccataa acagaatagc gctctttaat ttgaagggcg tacttatcaa gatattcacg
     2041 aatagccgct atgtaatcgg ggcttttagt tttcaaataa cctcccgaaa aaaacagatg
     2101 caactagtcc cgtgatacag agcactgtaa aaagtgtgtg aatccgaatt gtgcccataa
     2161 gagccttgtc gtaatcggga gtgccattat tggaaaaaat ttcttttata actggtattg
     2221 ccgccggcaa agagagcagg ctaagtaaaa gatacaatgt aaaaaaacca gatattgtgc
     2281 cgcaaacaat aataatatag ggcagcatca gagcagagag ggctacgagt gtagaagttt
     2341 tttttgcaaa atacatggga agcgttttta tatgcgcctt tttgtcattt tcaatatctc
     2401 ttaaattatt taaaaaaagt acaagcgccg ctagtaatcc aggtgccgag gccaaaaggg
     2461 cggcatgtag ggagaaggtt ctgctaacag catagagtga ggcaaggccc agaagcggac
     2521 cgaaagttat aaaaacagta agttcgccaa gagccatata tttaagactt aattttttac
     2581 ctgtataaaa aaacgagagt acagcacctg ttgttcccat gataagaagg ggcagtccgc
     2641 ttaaatagac aagaatggct ccgaaagacg cgccaataaa aaatagcaac aaggcatagt
     2701 gcagtaaaaa tctgggagcg tttctctgta tggcaagctg tctttcgttc ttgtctacgc
     2761 ccttgttata gtcatagtag tcgttaagca tgtttacggc actgtgaaat agcagaacag
     2821 tcgcgcaaac cagaaaaagg cggactgtgt caattctgtg gccactgcta tatgcccatg
     2881 cagggccaat aagcgcggag cttattgata gcgggaaaga gtaggctcgt agtgagctaa
     2941 ggtagagaga gagttctttt atcatgtagt tgattaggtt cctgtttgtt tcagtttagc
     3001 tttgtattgt ttaggatttg tctcttttgt caacttttta ctaaaatgta gcaaaagctg
     3061 gattgttagc caggtgaata ggaataataa atggggacgg aggttgaatt agtcatccaa
     3121 tgtgacataa tgcaagttga agctgaattg ttagccactt ttctggattc ttagccagct
     3181 gaaaactcag aaaaaaatga aatatggtta aaattccctt gaaaatgatg ctaacataga
     3241 aggatggtaa ttttatgtac cacgtgtaac ataggttaga atatggtata tacagccata
     3301 ctgtcgatac tgatttagtt atcagcattt taaaatcatc cataatttag tcggagaaaa
     3361 ttatgttaaa gatactaaga acttttgctg aatactcccg tccatatcgt ggagccttta
     3421 ttctggccaa tacctgcatg gttataatgg acttaacctc ttaccttctt ccggttctta
     3481 taaaatatgc aaccgacact gtttttgacc gtgtgaccaa aactggtgat cttaaaattc
     3541 tctatattgt agggtcagca ataataatct ccggtgttgt gcgtgggctt ctttcacact
     3601 ttatgatacg ctcatactgg tacgtgggcg agtctgtagt aaaggatatt cgcaacagcc
     3661 tttacgaaaa gttgcagcat ctcggagcct ctttttatgc gaaagcacgc acaggagatc
     3721 tgatgtcgcg gcttacgacc gacattcagc ttatacgtaa tttttatgcg tttggagtcg
     3781 agcaccgttt gcgaatagtt ttaatatcgg ttacaatact gttaataatg ttatatcaaa
     3841 actggcggct tgcgcttgtt gtatacattt ttcttccgat tatctatttc gtgatagtct
     3901 atttcagtag taaacttcaa aaagctgtta tgcgcaagca tgagagtgct ggagccctta
     3961 actccacact tcaggaaaac ctgaccggta tacgtattgt aaaagccttt gcggttgagg
     4021 atgaacagaa aaagatcttc gaaaaaagta atctccggct gtttaacgcg gatcttgttg
     4081 tgactacttt acaggctcat cttaatccac tgttgcagct taccggtggc gcgggaactc
     4141 tggtaattct tgtatatggc ggttatcagg taattcaagg aagtatgact cttggaacgc
     4201 ttatcggttt tatgacctat ttaatgataa ttaggtttcc gctttttatt ctggcattta
     4261 atacttcgct tgtaaacctc gcagagggag cgtccaaacg cataaaagaa atcttaattt
     4321 tagaagatca gcgtgaaggt gacaatggta ctgttacaaa caagataaaa ggaaaactcg
     4381 aatttaaaaa tgttactttt ggttacgggg aaaggaagcc ggttttgcgc gatctatcgt
     4441 tttctattag agaaggtgaa agggtagcac tctttggttt aaacggatcg ggcaaaagct
     4501 cgctcatatc actgattccg cggttttact gccctgatag tggcaagatc ttacttgatg
     4561 acacacctct tcccgagtgg agccttgaag cattgcgtct tcagatcggc actgtgttgc
     4621 aagagacatt tttgttttca atgacaatag gtgaaaatat cgcgtttgga cgccccgacg
     4681 cttcgttgca gcagattaag gaggcagcag ccagcgcaca aatagataca tttatagaat
     4741 cgcttcctga aggatatgat acctatgtag gtgagtcggg ttccgggctt tcaggcggac
     4801 agcgccaacg aatagccatc gcccgtgcgc ttttacaaaa tccgcgtatt cttatcttgg
     4861 atgactgcac ctccagcctt gatccgcaaa ccgagcgtaa tattcaaaac gaacttcgta
     4921 accttatgag cggacgcaca acgttgatta tcgcacaacg gatatccact cttcgtcttg
     4981 cagatcgtat tatcgttctt gatcgtggca tgatacaaga cttcgattcg cacgaaaatc
     5041 ttttaaagaa aaatgaactc tataaggcta catatgagac gcagatggtc tgcagcgaat
     5101 ccattatcga agacagcttg agtatgaggt catagcatga aagaaaagaa taaaccggca
     5161 aatataaaaa cagataaaac ggctcttagg cttttatccc gattgtttcc atacataaaa
     5221 acaaatgtcg ccggtattgc aattatgtat cttttttcgt taataaatgt tgcagcgaca
     5281 attgccttgc cttttcttat ccaaaagggt a
//
LOCUS       DAOTGZ010000191         5284 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_3064
            NODE_18526_length_5284_cov_4.580226, whole genome shotgun sequence.
ACCESSION   DAOTGZ010000191 DAOTGZ010000000
VERSION     DAOTGZ010000191.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868088
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 5284)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5284)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:22:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,785
            CDSs (total)                      :: 2,751
            Genes (coding)                    :: 2,732
            CDSs (with protein)               :: 2,732
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 12 of 19
            Pseudo Genes (internal stop)      :: 5 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5284
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_18526_length_5284_cov_4.580226"
                     /isolate="AMR_MDS_3064"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..336
                     /locus_tag="P5123_11005"
     CDS             <1..336
                     /locus_tag="P5123_11005"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HRX16832.1"
                     /translation="KESKIYIFFQIKNQECWISFLNTPSPNNREIEGIPEEYASAVFE
                     PFYRIDKRVYDGYKTLDFGLGLTLADKIVRKHGGIIEIQNVEDHSNLAGKEKLKVMVT
                     ARLPLHITI"
     gene            complement(397..774)
                     /locus_tag="P5123_11010"
     CDS             complement(397..774)
                     /locus_tag="P5123_11010"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HRX16833.1"
                     /translation="MNKKTILVVDDTPDNLSVLYEILKNDYRVLVANGGEKALQIIRG
                     GASIDLVLLDILMPDIDGYQVCSQIRLDKSTKNMPVVFSSAIDEDEQKQKAEGLNVSG
                     YISKPYRKDKVLDEIARILSVLG"
     gene            complement(771..4544)
                     /locus_tag="P5123_11015"
     CDS             complement(771..4544)
                     /locus_tag="P5123_11015"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain-containing protein"
                     /protein_id="HRX16834.1"
                     /translation="MNFDIRACFDNIKSAVFVCDKEFKVLYCNIAAKNLLKKKYANNN
                     SLLSDYTTKKFVSQVNQNCKDNCASTIFECSIHNHAYSVTVDTAQDIDNPSKSSSPFY
                     IIKLTSQTFESAQKSDDDNANKVIGKVCPGKGTRHRGSHCAEFELITTLAGIGYWEYD
                     CQTGDVEVSDILLQLAGAPSKARDNLSEYWMSHLHPDDRSKVFAEFKSAQKGDNELLY
                     LEYRIFNENIQRWSWMSAMGKVLKRDSNNMPLKFAGYQKDVSERKNFEKQLKKDSDYL
                     NMIMETTDVGYVVATFDGRILQYNNALLKVFNRTRDEIENGSFYRFLTESSRQDVKDN
                     FKKYIETGENTPAYEIEVYAPDGTIINCLVNPTPVQDESGDYIGSFAWLTDITGFKII
                     TRSLRETGNKLNIALQSAGLGMYEIDLSSGNVWWDKRMYEIYDRDPREFIPTIDSIVD
                     LVVPEDRDYENSIFFHTLQESTDWHQKYRIMDRRGNFRYIEGYGTIIENSDGVPQKVI
                     GVVSDMTERRMSERQKIKNSQQQLIIFDNSPLCMVFIDRNGFVLQSNSNFEKIFNVSF
                     DQVNIEKIDKLNNEQFSNFVKRAREGEDIETEIELDSDNKKVILHLILKPVLPGGMSD
                     VIGILEDITVRKTAESRLKAAILQAEEANRSKSAFLANMSHEIRTPMNAVIGLTYLLK
                     QTNMTTRQMDYASKIESASNNLLALINDILDFSKIEAGKIVVEQIPYSIHSIIDDIKT
                     IAQFNADQKKLSLDISIEDSVPRTVIGDPVRVHQIVLNLLNNAIKFTDHGIVTLTVTG
                     VQPNLKRKRITIIYRVTDTGIGMSKEQQRHIFESFTQADSTTTRNYGGTGLGLAICKG
                     LVMNMNGTISIKSRPGRGSTFTVSIPCRVSDMSLVQSESNNCKSSQEIRNENNEICSL
                     QGLGLLLVEDNTINQQVASELLEKEGIDVTVAQNGKEAIDVMEKRGWDSFDLILMDLQ
                     MPVMDGYEATKKIVTKTKTPIVAMSADVVHGVRERVQSAGMCDYIAKPINVSQLYRVI
                     AAYTNRKPVLTQSKVNEQSPMPQVKRQTGINSENALNNLNGNIELYCSLLESFLGSMS
                     RYPVQVEGMLHEGKVNDALRLIHTMKGVAVNLGLEGLEPLCADAQSAIKQSGAFNEQI
                     KVSLNQIAKYMNSISISIGQFISNNRNTIKILKPKTKLSDFINTMDELISFIKVYDGE
                     AVDIIKKLELMSLPENIKKEVEELSGLVLKFDFEKALEFAMRITTKYKNGFDK"
     gene            complement(4689..>5284)
                     /locus_tag="P5123_11020"
     CDS             complement(4689..>5284)
                     /locus_tag="P5123_11020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012843644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRX16835.1"
                     /translation="PGYYYITICTYNRECIFGKIVNGKMILNQYGQIADNEWLKTGQM
                     RRNIDMGQYIIMPDHIHGIIIIRDYPGGCVGPVGPVDPVGPVGPVGSVDSVGPVDPVD
                     SVDPGRVTMHRDPTGTQTGTQTGRVFEQFGKPTSNSIPTIIRGYKSAVTNRINELRNM
                     PCKPVWQRNYYEHIIRDDKSFWRISRYIKNNPVMYNP"
BASE COUNT         1527 a         1107 c          919 g         1731 t
ORIGIN      
        1 aaagagagca agatatatat cttctttcag ataaaaaacc aggagtgctg gatatcgttt
       61 cttaacacac cttcaccaaa caaccgtgag atagaaggaa ttccagaaga gtacgcttct
      121 gcggtttttg aaccatttta ccgaatagac aaaagagtct atgacggcta taaaacactt
      181 gattttgggc tggggcttac ccttgccgac aagattgtca gaaaacatgg cggcataatc
      241 gaaatacaaa atgtggaaga ccatagcaac cttgccggaa aagaaaaact taaagttatg
      301 gtgacagccc gtttaccgct gcacataact atttaaaaca ttatagcaat ctctaataat
      361 taaattatcc ggatgtatta aaaactgtag gcgttattat cctaaaacag ataagatgcg
      421 agcaatctca tcaagaactt tatctttgcg gtaaggcttg ctaatataac ctgaaacatt
      481 taagccttcg gccttctgtt tttgttcatc ctcatcaata gctgatgaaa aaactacagg
      541 catatttttt gtacttttat ctaatcgtat ttgcgaacaa acctgataac cgtcaatatc
      601 tggcatcagt atatcgagca gcaccaaatc aatagaagcc ccgcctctga taatttgcaa
      661 tgccttttct ccgccattgg ctaccagcac tcgataatca ttttttagta tctcataaag
      721 caccgatagg ttatccgggg tatcatctac aaccagtatg gtttttttat tcatttatca
      781 aatccgtttt tatattttgt tgtgattctc atagcaaatt caagagcctt ttcgaaatca
      841 aattttagca caagtccgct aagctcttca acttcttttt taatattttc aggaagactc
      901 attaattcaa gttttttaat aatatcaaca gcctctccat cgtaaacttt tatgaagcta
      961 atgagttcat ccattgtatt tataaaatcg cttaatttag tctttggttt caagatcttt
     1021 atagtatttc tattgttact tataaactgg ccaatgctta tacttatgga attcatatat
     1081 tttgctatct gatttaaaga tacttttatc tgttcgttaa aagcacccga ttgtttaata
     1141 gcagactgag cgtccgcaca aagaggttca agaccttcaa gccccagatt tactgccacc
     1201 cctttcatgg tatgaatgag ccgtaacgcg tcatttacct ttccttcatg tagcattcct
     1261 tctacctgta caggatatcg gctcattgac cccaaaaaac tttctaacaa agaacagtat
     1321 agctcaatat taccgttaag attgttcaaa gcattttcac tgttgatgcc tgtttgtctt
     1381 ttaacttgag gcatcggaga ttgctcattt actttacttt gagtcaaaac tggctttcga
     1441 ttggtatatg ctgcgatcac acgatagagc tgactgacat taattggttt tgcaatatag
     1501 tcacacatgc ctgccgattg aacacgttct cgtacgccat gtactacatc cgcgctcatt
     1561 gccacaattg gagttttggt tttagtaaca attttttttg tggcttcata tccgtccata
     1621 accggcattt gcaagtccat taatattaga tcaaaactgt cccatcccct tttttccata
     1681 acatcgatgg cctctttacc gttttgcgcg acggttacat caataccttc tttttcaaga
     1741 agttctgatg caacctgttg attaattgta ttatcttcta caagaagcag acctaacccc
     1801 tgtaaagaac atatttcgtt attttcattt ctaatttctt gcgagctctt gcaattattt
     1861 gattccgatt gaactagaga catatccgaa acgcggcatg gtattgaaac ggtaaaagta
     1921 ctccctcttc cgggccggct tttaatcgaa attgtgccat tcatattcat aaccaggcct
     1981 ttacagatag caagaccaag cccggtaccc ccgtaattac gggttgttgt agagtcggcc
     2041 tgagtaaagg attcaaaaat atgacgttgt tgttccttac tcatacctat accggtatct
     2101 gttacgcgat atataatggt tattcttttt cgcttcaagt taggttgaac gcctgttacc
     2161 gtaagagtca ctattccatg atctgtaaac tttatcgcgt tattcagaag attaagcaca
     2221 atctgatgaa cacgaacagg atcaccaatc acagtgcgcg gaaccgaatc ttcgatagag
     2281 atatcaagac tcagtttctt ttgatctgcg ttaaactgtg ctattgtctt tatgtcatca
     2341 ataatagagt gtatgctata aggaatctgc tctacaacta tcttacccgc ttctatcttt
     2401 gaaaaatcca gaatatcatt tattaatgcc aacaagttat tggacgcact ctctatcttg
     2461 gacgcgtaat ccatttgtct tgttgtcata tttgtctgct taagaagata ggtaagaccg
     2521 ataaccgcgt tcataggtgt gcgaatttca tggctcatat ttgctaaaaa agcgcttttt
     2581 gacctgttgg cctcttccgc ctgtagtatt gccgccttaa ggcgactctc ggcagtttta
     2641 cgtactgtaa tatcttctaa aataccgatg acatctgaca ttccgcctgg caaaaccggt
     2701 ttaagaatta aatgaagaat gacttttttg ttgtcactat ccagttctat ttcagtttca
     2761 atatcttcgc cttctctcgc acgtttaaca aaatttgaaa actgttcatt attcagctta
     2821 tctatttttt ctatgttaac ctgatcaaaa ctcacattga aaatcttttc aaaattacta
     2881 ttagactgca aaacgaaacc gttgcgatct ataaagacca tacaaagagg agagttatca
     2941 aaaattataa gttgctgctg actgttcttt attttttggc gttcagacat tcttctttca
     3001 gtcatgtcag aaactacacc aataaccttt tggggaactc catcactatt ttcaattatt
     3061 gttccataac cttctatata tctaaaatta cctctgcgat ccataatacg atacttttga
     3121 tgccagtcag ttgattcttg taatgtatgg aaaaaaatac tgttttcgta atctctgtct
     3181 tcaggaacaa caagatcgac tattgaatct atggtaggta taaattcacg gggatcacga
     3241 tcatatattt cgtacatacg cttatcccac catacattac cgcttgaaag atcaatctcg
     3301 tacattccaa gaccagctga ctgaagagca atattaagtt tgttacctgt ttcacgaagg
     3361 cttctggtaa taatcttgaa accggtaatg tcggtaagcc aggcaaaaga tcctatatag
     3421 tctccgcttt catcctgaac cggtgtcgga ttaacaagac agttgattat ggtaccgtca
     3481 ggagcataaa cctcgatctc gtacgcagga gtattctcac ctgtttcgat atatttttta
     3541 aaattgtcct taacatcttg tcgactggac tcggttaaga atctgtaaaa actaccattt
     3601 tcgatctcgt ccctggtacg gttaaaaacc tttaaaaggg cattgttata ttgtaatatt
     3661 cttccgtcaa aagttgctac aacataaccg acatcggttg tttccattat catattcaaa
     3721 taatcgctat cctttttgag ctgcttttca aagtttttac gttcggaaac atctttttga
     3781 taacccgcaa acttaagagg catgttgttg gaatctcttt tgagaacctt ccccattgcc
     3841 gacatccagc tccaacgctg aatgttttca ttgaatattc tgtattcaag atataaaagc
     3901 tcattatcac ctttctgagc cgacttaaac tcggcgaata ccttgcttct atcatcagga
     3961 tgcaggtggg acatccagta ttcggataag ttatcacgtg ccttagaagg tgctccggca
     4021 agttgaagca gaatatcaga tacctctaca tctccggttt gacagtcata ctcccagtac
     4081 ccaattcccg caagtgttgt aataagctca aattcggcac agtggctacc tcggtgacgc
     4141 gtgcctttcc ctgggcatac ttttccgatg actttattag cattatcatc atccgatttt
     4201 tgagccgact caaacgtttg cgaagtcaat ttaattatgt aaaaaggaga gctgctcttt
     4261 gagggattgt ctatatcttg agcggtatct acagtaacag aatatgcatg attatgtatt
     4321 gaacactcaa atatggtcga agcgcaatta tctttgcaat tttgattaac ctgagacaca
     4381 aatttctttg ttgtataatc actaagcaaa gagttgttgt ttgcatattt tttctttaaa
     4441 agatttttag cagcgatatt gcagtacaat accttgaatt ctttatcaca aacaaaaaca
     4501 gcactcttta tattatcaaa acaggctctt atatcaaaat tcatcggctc ctcatttagg
     4561 tttatccgtt tttttaatat aacaacaaaa accgtaataa gcattaaaga gttaccaaaa
     4621 atggaaaaat attatctgtg atgtggggtc actggtttgt ggggggcacg gcatgctgtg
     4681 accgataatc atggattata cattaccgga ttgtttttaa tatatcgcga tattctccaa
     4741 aacgatttat catcacgaat tatatgttcg taataatttc gttgccatac cggtttgcag
     4801 ggcatattac gtaattcatt aatccggttt gtcaccgccg atttatatcc gcggattatt
     4861 gtcggtatcg aatttgatgt cggtttacca aattgttcaa atacccgtcc ggtttgtgtc
     4921 ccggtttgtg tcccggtagg gtcacgatgc atcgtgaccc taccgggatc aacggaatca
     4981 acgggatcaa cgggaccaac ggaatcaacg gaaccaacgg gaccaacggg accaacggga
     5041 tcaacgggac caacgggacc aacgcatccg ccgggataat cccggatgat tattataccg
     5101 tgaatatggt cgggcattat tatgtattga cccatatcga tattgcgccg catttgaccg
     5161 gtttttaacc attcattatc cgcgatttga ccgtattggt ttaatatcat tttaccgttt
     5221 acgattttac cgaatatgca ttcccgatta tatgtacata ttgttatgta ataataaccc
     5281 ggcc
//
LOCUS       DAOTGZ010000192         5260 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_3064
            NODE_18639_length_5260_cov_6.147935, whole genome shotgun sequence.
ACCESSION   DAOTGZ010000192 DAOTGZ010000000
VERSION     DAOTGZ010000192.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868088
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 5260)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5260)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:22:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,785
            CDSs (total)                      :: 2,751
            Genes (coding)                    :: 2,732
            CDSs (with protein)               :: 2,732
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 12 of 19
            Pseudo Genes (internal stop)      :: 5 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5260
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_18639_length_5260_cov_6.147935"
                     /isolate="AMR_MDS_3064"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            553..2637
                     /locus_tag="P5123_11025"
     CDS             553..2637
                     /locus_tag="P5123_11025"
                     /inference="COORDINATES: protein motif:HMM:NF024683.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chitobiase/beta-hexosaminidase C-terminal
                     domain-containing protein"
                     /protein_id="HRX16836.1"
                     /translation="MTRIVKYFLIALLGFGMLAGCDSCFDDELTDKVDAPNFTLYRNT
                     NYDVVLSSDTKGATIKYTLDGSDPKDSNTAIEGTELTIDFFTNIRAYAYKKGKRDSIT
                     VDFSLPRFGGKRKIFYNSDYTVKSYVNSSNDCNNKRKLEVFASEKGSDNQWFTYDDKI
                     DYYKLYNYDQDTLKECVVYNDPGEDDKWFTSDDQIDKTYDVSISGNDYSFGGEYTEKF
                     DDQMQLVETNEYSDGPYVYTYDGRKPVDFTDGAGNTLVYQLEDNQEYYYLYENNSDGK
                     PDKKIKYLGYGTDTNFGLTKNSYNEDGTLKSSTYYWDNAAKKERAHYIYAYDQGKLVS
                     ETKYKASDKEKLRFWREFEYEDNGAIKRIDYFDKDEARTIYEEFVYNGDLTASMTRYQ
                     RPAVAYKSFKFDFEVTANVVDGGYFIYAGGKFDHEGDPIEDIVDMGEDEQEFIENMTE
                     KVKDIIKNVELKKASVILNGKLGGNAKILVATYDNTINIVFEFNSYNDGDVIINGTID
                     KELTGTIDVSGELGGGTAAGNGKVYGELMIADANPDFTDEDYIICAYSFIELDDQNRF
                     VKETEYDTPGSDGQWDTISDNSIKYIFNKTFSGDVLTEHNCFNRTEDVANLNYRIVYE
                     ESSGVVTKETVTRNYGAYGKQDSGSTNETLEYYRCWSHLWDIQQRWEIDWDDEYWNED
                     VDKKDRVEWCVE"
     gene            complement(2756..3580)
                     /locus_tag="P5123_11030"
     CDS             complement(2756..3580)
                     /locus_tag="P5123_11030"
                     /inference="COORDINATES: protein motif:HMM:NF012451.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="J domain-containing protein"
                     /protein_id="HRX16837.1"
                     /translation="MVKNISEAYTLLQIKPGATNDEIAKAFKKMAMLYHPDKNRDKPE
                     WANSKMAAINSAYNFILGSRFATSEESVKKEPPPKTKPTDYFAQKILREQLIKSFVKV
                     REDTKEHLYRYYQFQLYNIARRDNVMNRSTFKDIVNRIKKSYHTVQSLSNNTKDEELI
                     SHFNVFNRMIFNFYKASECLNILDSYKNVLEVEAYNQYHIGDKYLHAAEIEVFYNRHN
                     RGKFFQGECVENAMNAKLIFLQTIRAFPKSTWAVETEIKLEFTESLLAYINLFFNE"
     gene            3833..4450
                     /gene="lexA"
                     /locus_tag="P5123_11035"
     CDS             3833..4450
                     /gene="lexA"
                     /locus_tag="P5123_11035"
                     /EC_number="3.4.21.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020774430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006282 - regulation of DNA repair
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor LexA"
                     /protein_id="HRX16838.1"
                     /translation="MSKDLTDRQREIFDFIKEIISGSGFPPTIREIGNRFEISVKGAY
                     DHLKAIEKKGYLRCTQNKSRAIELLTECELQYDDNTIRIPLLGTIAAGAPLLAEENIE
                     DYLSLPGPAFSSGQFFGLRVRGDSMIDEGIFDGDIAVIRKQETAINGEIIAALIGDEA
                     TLKTFSKKDGTIRLLPANEKYEPIVPKELVILGKLAGLFRIYRKG"
     gene            4648..5247
                     /locus_tag="P5123_11040"
     CDS             4648..5247
                     /locus_tag="P5123_11040"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRX16839.1"
                     /translation="MELTVGSLVQNSIKKGIKYVGPLVVNVFLFGLTWWIPYLNVGTA
                     IGLFWGIPTKMSRDEEILITEIFDPKYRKNMGEIFLTLSFVSSGVAIGALFMFVPALV
                     IQVSWSLATLLVADKEYGPIDAIKKSNDLTYGKKWTIFFGSLVLKILLFVACGILVSI
                     FSMIHSGLGVLVGLVCILFVASILLGFKGELYGTLAKDA"
BASE COUNT         1647 a          756 c         1106 g         1751 t
ORIGIN      
        1 gtaaataata ctatgatttg gctttaaacg gtatagtcgt tccagaaatg gtaatatttc
       61 tatgtttcca tatgcctgtt cttatgaaat agacaggttt tttataattg tcattggatt
      121 tattcataaa aatttgtcat caatagaggt caataatccg cacaggcggg cttgatattt
      181 tatcttaaat atggaatatt aatctatttt gagttgacaa acggtcgaga acttgtatta
      241 ttaaaacatg tttaaaaagt ttatctgtgt tacaaagaag tggtatcatg ttatactagc
      301 aaattttctg tttaaagaat caaaggtttt ataagttttt tccaatcttc tatgttcttt
      361 gccgcaaagg aactttgata aatttcgatt ttaaaacggt cacgcgcttt tatttccgtt
      421 ttgatagaaa tttctcgtaa gtgccaagct agtgccgtaa ttagttgagg ttttcattga
      481 gttctcttgc cgattattca gatttaataa taaaacagaa gttcttgtga cttctaatgg
      541 tttggagggg ttatgaccag aatcgttaaa tattttctaa ttgccttgtt gggctttggc
      601 atgcttgccg gttgtgatag ttgctttgat gacgagctaa cagataaggt tgacgcacct
      661 aattttaccc tttacaggaa cacaaattat gatgttgtac tttcttcgga tacaaagggt
      721 gcaacaatta agtacactct tgacggatct gatccgaaag atagcaatac tgctatagaa
      781 ggtactgaac ttacgatcga ttttttcacc aatatcaggg cgtacgcgta taaaaaaggg
      841 aagagggatt caattaccgt agatttttca cttcctcgtt ttggaggaaa gagaaagatt
      901 ttttataatt ctgactatac tgtaaaatct tatgtgaatt cttccaacga ctgtaacaac
      961 aaacgtaaac ttgaagtttt tgcctcagaa aagggatctg ataatcaatg gttcacatat
     1021 gatgataaaa ttgattatta caaactgtac aattatgatc aggatactct taaagagtgt
     1081 gttgtatata atgatcccgg tgaagatgat aagtggttca ccagtgatga tcagatagat
     1141 aaaacttacg atgtttcgat aagtggaaac gattacagtt ttggtggtga gtatactgaa
     1201 aaatttgatg atcaaatgca gcttgtcgag acaaatgagt acagtgacgg accctatgta
     1261 tacacatacg atggtcgaaa acctgttgat tttacagatg gagccggaaa tactttggtg
     1321 taccagctgg aagataatca agaatattac tatctctatg aaaacaatag tgacggaaaa
     1381 cccgataaga agatcaagta tttgggttat ggaactgaca caaactttgg tctgaccaag
     1441 aacagttaca acgaggatgg aactttaaaa agctccacat attactggga caatgcggct
     1501 aagaaagaga gagctcatta tatctatgca tatgaccagg gtaaactggt tagcgaaacc
     1561 aaatacaagg cttccgataa agagaagttg cgcttttggc gtgagtttga gtatgaggat
     1621 aacggcgcaa ttaaacgaat tgactatttt gataaagatg aagcgcgtac aatatacgaa
     1681 gaatttgttt ataatggtga tttaacagcg tcaatgacaa ggtatcagag gcctgctgtt
     1741 gcttataaaa gcttcaagtt tgattttgag gtaacagcta acgttgttga tggtggatat
     1801 tttatctatg ctggcggaaa gttcgaccat gaaggcgatc caattgaaga tatagttgac
     1861 atgggtgaag acgaacaaga atttatcgag aatatgacag agaaggttaa ggatatcatt
     1921 aagaatgttg aattaaaaaa ggcatcggta atccttaatg gtaaattggg tggaaatgcc
     1981 aagattcttg ttgctaccta tgataatact atcaatattg tattcgagtt taacagctat
     2041 aacgatggtg atgttattat taacggaact attgacaaag aactgaccgg tacaatagat
     2101 gtttcgggtg agcttggtgg tggaacagca gccggcaatg gaaaagtgta tggcgagctt
     2161 atgatcgcgg atgctaatcc tgattttacc gatgaagatt atatcatttg cgcttactct
     2221 tttattgagt tggatgacca gaaccgtttt gttaaagaga cagagtacga tactcccgga
     2281 tcggatgggc agtgggatac tatttcagat aacagcatca agtatatatt taacaagact
     2341 tttagtggag acgttttaac cgaacacaac tgttttaacc gtacggaaga tgttgctaat
     2401 ttaaactatc gtatcgttta tgaagagtct tctggtgttg ttactaaaga gacagtgact
     2461 aggaactacg gtgcttatgg aaaacaggat tcaggtagca caaacgagac tcttgaatat
     2521 tatcgatgct ggagccatct ttgggacatt cagcaaagat gggaaataga ctgggatgat
     2581 gagtactgga atgaagatgt tgataagaaa gatagagttg agtggtgtgt tgagtaaata
     2641 acagctaaag ctgtaaaaga taaataaaaa aggagccttg gctccttttt tatttatctt
     2701 tataattaca gagagtagtt gattatggtt tcaaataata tttttgtatt atagactatt
     2761 cgttaaaaaa aagatttatg tatgctaaaa gcgattctgt aaattccagc ttgatttctg
     2821 tttcgactgc ccatgtcgat tttggaaagg ctctaattgt ttgtaaaaaa atcaatttag
     2881 cgttcattgc attctcgaca cattcacctt gaaagaattt gcccctattg tgacggttat
     2941 aaaaaacttc aatttcggcc gcatgcagat atttatcacc tatatgatac tgattgtatg
     3001 cttcaacttc gagtacattt ttataagaat caagaatgtt aaggcattcc gacgccttgt
     3061 aaaagttaaa gatcattctg ttgaatacgt taaaatgcga aatcagttcc tcatctttgg
     3121 tattgttgct cagagattga acagtatgat aagacttttt tatacggtta actatgtcct
     3181 taaaagtcga tctgttcata acattatcgc gacgtgcaat attgtacagt tgaaattggt
     3241 agtagcggta taagtgctct ttggtatcct ctcttacttt tacaaaggat ttgattaact
     3301 gttcccgtag tattttttgt gcgaaataat ctgtgggttt cgttttagga gggggctctt
     3361 tctttacaga ttcttcagaa gttgcaaaac gtgatccaag tataaaattg taagcgctgt
     3421 taatagccgc cattttgcta ttggcccact ccggcttgtc gcgattctta tcgggatgat
     3481 agagcatcgc cattttttta aatgcttttg cgatttcgtc atttgttgca ccgggcttta
     3541 tctggagaag agtgtatgct tcagaaatgt ttttaaccat tttttctccg gaattaaaat
     3601 atataaatga gcaaatataa gacattttgt ataaacaaaa actatttgta aagcaaatat
     3661 ggccactagt aagatttcgt gtgtatgtgc taaatttagt tagcgaagca agcgtttata
     3721 gcaaaaaaaa ctaaaaaatt atgccatcat aattacacat cgcttatatt ctctttttgc
     3781 ttgacatcta tactcatgtt tgctatacgt atgtagtgca aaaggaggat ttatgagtaa
     3841 agaccttacg gatcgtcaaa gggaaatctt tgattttatt aaagaaatta tttcgggttc
     3901 tggctttcct ccgactatta gggaaattgg caatcgcttt gaaatctctg ttaaaggtgc
     3961 ctatgatcat ttgaaagcga tagagaaaaa aggatatctt cgttgtacac aaaataaatc
     4021 cagagctatc gagttactca ctgaatgcga gcttcagtat gatgataaca caatacgtat
     4081 acctctttta ggtacaatag cagcgggtgc tccgcttttg gcagaagaga atatagaaga
     4141 ttatcttagt ctaccaggtc ctgcttttag ttcggggcag ttttttgggc ttcgtgtgcg
     4201 aggagacagt atgatagatg agggaatttt tgatggagat attgcagtta tccgaaaaca
     4261 ggagactgct attaatggtg aaataattgc cgctctgatc ggggatgaag caactttaaa
     4321 gacctttagt aaaaaagatg gtactattag gcttttaccg gcaaatgaaa aatatgagcc
     4381 gatagtacct aaagaattgg ttatattggg aaagttggcg ggattgttta gaatatatcg
     4441 taagggttaa gtattattgt agttatatga tggtattttc gaatatattc gtgaccaatt
     4501 tttttgtgct gttctttctc aaagatgtac ttgctgcttt tcatgagtca aaaaatacta
     4561 ttatttactt gattatgctg aaaaaaactt tacttttaaa tttatccctg ttattttata
     4621 acaaataaaa attaaggaga gagagcaatg gaattaactg taggttcttt ggttcaaaac
     4681 agtattaaaa aagggatcaa gtatgttggt ccacttgtag taaatgtttt tctttttggg
     4741 ttaacatggt ggattcctta tcttaatgtt ggaaccgcta ttggtttgtt ttgggggatc
     4801 ccaacaaaga tgtcaagaga tgaggaaata cttataactg aaatatttga ccctaaatac
     4861 cggaaaaata tgggtgaaat ctttttaaca ttgagctttg tttccagtgg tgttgctatt
     4921 ggtgcgctct ttatgtttgt acctgcattg gttatacagg tatcttggtc attagcaaca
     4981 ctgttagtgg cagataaaga gtatggccct attgatgcga taaagaaaag taatgattta
     5041 acatatggaa aaaaatggac tatctttttt ggtagtcttg tattgaaaat attgttattt
     5101 gttgcgtgtg gaatattagt gtcaattttt tctatgattc attctggctt gggagtacta
     5161 gttggtttag tttgtatcct ttttgtagcg agtattcttc taggttttaa gggtgaactt
     5221 tacggaacac ttgctaaaga tgcttaataa ataaccattt
//
LOCUS       DAOTGZ010000193         5251 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_3064
            NODE_18682_length_5251_cov_5.432063, whole genome shotgun sequence.
ACCESSION   DAOTGZ010000193 DAOTGZ010000000
VERSION     DAOTGZ010000193.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868088
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 5251)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5251)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:22:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,785
            CDSs (total)                      :: 2,751
            Genes (coding)                    :: 2,732
            CDSs (with protein)               :: 2,732
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 12 of 19
            Pseudo Genes (internal stop)      :: 5 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5251
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_18682_length_5251_cov_5.432063"
                     /isolate="AMR_MDS_3064"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            256..459
                     /locus_tag="P5123_11045"
     CDS             256..459
                     /locus_tag="P5123_11045"
                     /inference="COORDINATES: protein motif:HMM:NF014021.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase domain-containing
                     protein"
                     /protein_id="HRX16840.1"
                     /translation="MNQLCFPHDSSLYLFGSRTDDTKKGGDIDLFLESNSTVDPKSEI
                     DFLKLIYKNATQRKIDLIVKTPE"
     gene            501..704
                     /locus_tag="P5123_11050"
     CDS             501..704
                     /locus_tag="P5123_11050"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRX16841.1"
                     /translation="MVMLNDSIDTAFLHYNRAKKNHNIIRNDNLNREVFLDDNKIKTI
                     DAFNIMEEILLSVKKYCRKKNLL"
     gene            815..1138
                     /locus_tag="P5123_11055"
     CDS             815..1138
                     /locus_tag="P5123_11055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011368001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease adaptor ClpS"
                     /protein_id="HRX16842.1"
                     /translation="MARIFNTIYNSSEEIEAELNHELEEPSMYTVIMHNDHYTTMPFV
                     VEVLKKVFHKSAEEANNLMMQIHTEGSTVCGIYIYDIANTKVSHVHALAKHAGFPLRC
                     SMEEV"
     gene            1138..3426
                     /gene="clpA"
                     /locus_tag="P5123_11060"
     CDS             1138..3426
                     /gene="clpA"
                     /locus_tag="P5123_11060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010248394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009368 - endopeptidase Clp complex
                     [Evidence IEA];
                     GO_function: GO:0004176 - ATP-dependent peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA];
                     GO_process: GO:0043335 - protein unfolding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit
                     ClpA"
                     /protein_id="HRX16843.1"
                     /translation="MRINQELELILKSSFQEAIHRNHEYLSPEHVIYTSLFFSSSHEL
                     FEDLDIDIEVLRDELNNYLNEKVPRVQGATEREPVETVALQNSMQRAYIHVESAHKGE
                     IDFGDILVAIYEEEGCFARFLLAKQGLTKLDILEYISHHSKFEPKPENDFEDDDEDLY
                     DKKNEDRKKSDSFLEKYTEDLTQKARDGQTEPLIGREDILQRTMQVLCRRLKNNPIHV
                     GEPGVGKTAITEGLAELIANGEVPEALKDSSIYSIDLGSMLAGTKFRGDFEERLKRVI
                     KELEKKSNVIIFIDEIHTVVGAGSVSGGSMDASNLLKPALASGKIRCIGSTTFEEYRK
                     FFEKDKALVRRFQKINIPEPTAKETGKILEGLKSRYENHHNVIYTSDALKAAVDLSIK
                     YLHERHLPDKAIDLIDEAGAFVQLNHKNDKNEETVTIDRKAIEKVVALMTRIPEESLS
                     EGESGKLKNLKKRLKDNIFGQDHAIEELVSAILRSRAGFTDDTKPVASLLFVGPTGVG
                     KTELARQLSEQMAIELHRFDMSEYQEKHTVARLIGAPPGYVGYDEGGQLTEAIRKTPH
                     CVLLLDEIEKAHSDIFNTLLQVMDHATLTDNNGKKADFRNVIIIMTSNAGARDLGKAR
                     VGFTDQTNTGAVDTAVKEFFSPEFRNRLDAIIKFENLSRAMVLRIVDKELDIFRQKLS
                     AKEISLNISKNCREWIADKGFSAEFGAREISRVIQNSFKKPLAEKLIFGILAKGGEIK
                     ATLKNNEVKFSFKAKIEETTRV"
     gene            3432..4160
                     /gene="aat"
                     /locus_tag="P5123_11065"
     CDS             3432..4160
                     /gene="aat"
                     /locus_tag="P5123_11065"
                     /EC_number="2.3.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012676692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008914 - leucyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="leucyl/phenylalanyl-tRNA--protein transferase"
                     /protein_id="HRX16844.1"
                     /translation="MLYKLDDNLIFPDPNKAPADGLLAYGGDLTTDRLLFAYRSGIFP
                     WYNEEPILWWSPNPRFVVFPDKVYVSRSMRKILEKNVFTITVDTAFEQVINNCADIPR
                     AGQPGTWITEEMIEAYCRLFDEGYAHSFEAWKNGELVGGLYGLSMGSIFFGESMFTFA
                     SNASKAAFLSMASFVKKMGFSLIDSQVHTPHVETLGGESISRRKYLEILKNGLQQNDV
                     TGSWSELYSSVTSSGTTLATEDPR"
     gene            complement(4106..>5251)
                     /locus_tag="P5123_11070"
     CDS             complement(4106..>5251)
                     /locus_tag="P5123_11070"
                     /inference="COORDINATES: protein motif:HMM:NF024299.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HRX16845.1"
                     /translation="EAAKLYREASLIQERNDRESINRAIDLYTRVLARYPETRAAIDS
                     TFKIAESYEKVDLYKLAYLKYSYLLKNPYVMANKEKKNEVLARMAALKIKRNYSEEGL
                     NQLYTVLNNSTDSEFRSRIYSEIGYTHLRLGNVDKADNAFDLALRENHDNEEALLGKA
                     RSLRRLGKNSEAFKKYDYFLAHHGAFSSYTPDVKRAYLDQAYNAGLSSYRSASFWPAI
                     NYFGIVLKNFPASKYSENALYWTAESYYSLGRYSRAIGYYNKTLSNGLYHKDEDARIK
                     KGYAYFMQKKYDLAAREFQLYLEKYPRGKHRHIATRWKETSAQELMQKIRKETVEAEP
                     VPEPREVEPRTRYEVVPESKYEEAESGKDVYLGSSVAKVVPDDVTEL"
BASE COUNT         1689 a         1119 c         1005 g         1438 t
ORIGIN      
        1 gaagcgaatt cgctcaatgc ctctttcagt attctctctt tagtatcagc gcccttaata
       61 gccatacaaa cctaccaact gtttggttaa tttacaaaat atataataaa ctgtcaacaa
      121 aaactctgac taaatataaa ctttttttaa tcttaaaacg gcaagagatg acgttataag
      181 ataactcttt tgacttgaca tcatataaac ctaagttaaa cttaaaatta gctagtaaac
      241 atactttgga caataataaa ccaactttgt ttcccgcatg attccagcct ctatctcttt
      301 ggcagtcgca cggatgacac aaaaaaaggt ggcgacatag atctttttct ggagagtaat
      361 tcaactgttg atccaaagtc agaaattgat tttttaaaac ttatatacaa aaatgcaact
      421 caacgaaaga ttgatcttat tgttaaaaca cccgaataac actcaagcaa tatttataat
      481 acagcaaaaa ctgaacgaat atggttatgc tgaatgactc tatagataca gcctttttac
      541 attataatag agctaaaaaa aaccacaata ttattcgtaa tgataatctt aatagagaag
      601 tttttcttga tgataacaaa atcaaaacca tagacgcttt taatattatg gaagagatat
      661 tattatcagt aaaaaaatat tgccgcaaaa aaaatctgct ttaaacaaga gttacacatt
      721 ccactttatc atcactttat tgcttgcagg cttgacattt tattgctata tatataaaag
      781 aacataaagc aacattcatt ataatggaat cgaaatggca agaatattta atacaattta
      841 caacagttcc gaagagatag aagcggagct taaccatgaa ctcgaagaac cttctatgta
      901 cacggtcatc atgcataacg atcactatac cacaatgccc tttgttgtgg aagttctaaa
      961 aaaggttttt cataaatcag ccgaagaggc aaataacctt atgatgcaga ttcataccga
     1021 aggatcaaca gtttgcggca tatatatata cgatattgcc aacacaaaag tcagccacgt
     1081 gcacgcgctt gcgaagcacg ccggttttcc gctgcgatgc agcatggaag aggtataatg
     1141 agaataaatc aggaacttga actaatatta aaatcatcct ttcaggaagc tattcaccgt
     1201 aaccatgaat atctgtcgcc ggaacatgtt atttacacat cactcttttt ctcaagcagc
     1261 cacgaacttt ttgaagatct tgatatagat atcgaagttc tccgggatga gcttaacaat
     1321 taccttaacg aaaaagtccc aagagtgcaa ggtgcgaccg agcgcgaacc tgttgaaaca
     1381 gtagctctgc aaaacagcat gcaaagagcc tatattcatg tagaatcggc acataaaggc
     1441 gaaattgatt tcggagatat tcttgtagca atttacgaag aagagggctg tttcgcccga
     1501 tttcttttgg caaaacaggg tttaaccaaa ctggatatac ttgaatatat ctcgcatcac
     1561 tctaaatttg aaccaaaacc cgaaaacgat tttgaagatg acgacgaaga tctttatgat
     1621 aaaaaaaacg aagaccggaa gaaaagcgac tcttttctgg aaaaatatac cgaagatctt
     1681 acacagaaag ctcgtgatgg acaaacagaa cccttaatcg gtcgtgaaga tattttacag
     1741 cgtacaatgc aagttctttg taggcgacta aagaacaacc cgattcatgt tggtgaacct
     1801 ggtgtaggca aaaccgctat taccgaaggt ctcgccgaac ttattgccaa cggtgaagtt
     1861 cccgaagccc ttaaggattc atcaatttac tcaatcgatt taggctccat gcttgccggt
     1921 accaagtttc gaggtgattt tgaagagagg ctcaaacgag tcattaaaga gctcgaaaaa
     1981 aaatcaaatg ttattatttt tatcgatgag attcacaccg ttgtcggagc cggctctgta
     2041 agcggcggat caatggacgc ttctaatctt cttaaacccg ctctcgcatc tgggaaaata
     2101 agatgtattg gatccactac ctttgaagaa taccgaaaat tctttgaaaa agacaaagca
     2161 cttgtgcgcc gctttcagaa gatcaacatt cccgaaccaa ctgcaaaaga gacgggtaaa
     2221 attcttgaag gattaaaatc ccgatacgaa aatcatcata acgttattta cacctccgat
     2281 gctcttaaag ccgcggttga tctttctata aaatatcttc atgaacggca tttacccgac
     2341 aaggctattg accttattga tgaagcagga gcctttgttc agcttaacca taaaaacgat
     2401 aagaacgaag aaactgttac gattgaccgt aaagcgattg aaaaagttgt cgcattaatg
     2461 acacgtattc ccgaagagag cctaagcgaa ggcgaaagcg gaaaacttaa gaatcttaaa
     2521 aaacgcctta aagacaatat ttttggtcaa gatcatgcta tcgaagagct ggtatccgca
     2581 atattaagat ctcgagccgg atttaccgat gacacaaaac cggtggcatc actgcttttt
     2641 gtaggaccaa caggtgttgg caaaaccgaa ctggctcgcc aactttcaga acagatggcg
     2701 atagaactac accgttttga catgagtgaa tatcaggaaa aacataccgt agcacgtctt
     2761 attggtgctc ctccaggata cgtaggctat gacgaaggcg gccagcttac cgaggcaata
     2821 cgcaaaactc ctcattgcgt actgctgctt gacgagatag aaaaagctca ctctgatatc
     2881 tttaacacac tgcttcaagt tatggatcac gccaccctga cggataacaa cggcaagaag
     2941 gctgattttc gcaatgtaat catcatcatg acatcaaatg ccggagcccg agatcttggt
     3001 aaagcccggg ttggttttac tgatcagaca aacacaggtg ctgtagatac ggctgttaag
     3061 gaatttttct cacccgaatt tcgaaaccgt cttgatgcta taataaaatt tgagaacctc
     3121 tcacgcgcca tggttttaag aattgtggat aaagagcttg atattttcag acagaaactc
     3181 tcggccaaag agataagctt aaacatatca aaaaactgcc gtgaatggat agccgacaaa
     3241 ggattttcgg ccgaatttgg cgctcgtgaa atatcccgag taattcagaa cagcttcaag
     3301 aagcctcttg cagagaagct tattttcggt atattagcga agggcggcga aattaaggct
     3361 accttgaaaa acaacgaagt aaaattcagc tttaaagcaa agatcgaaga gaccacccgg
     3421 gtataacctt tatgctttac aaactggacg ataatttgat ttttccggac cccaacaagg
     3481 caccggccga cggactttta gcttatggag gtgacttaac taccgacagg cttctttttg
     3541 cctaccggtc tggcatattt ccatggtaca acgaagaacc tatactttgg tggtcaccca
     3601 acccacgttt tgtggttttt ccagataaag tttatgtttc cagaagcatg cgcaaaattc
     3661 tggaaaaaaa tgtttttaca ataactgtcg acaccgcctt tgaacaggtt ataaataact
     3721 gcgccgacat cccacgagcc ggacaaccag gcacctggat aacagaagag atgatagaag
     3781 cctactgcag gctgtttgat gagggctatg ctcattcgtt cgaagcctgg aaaaatggag
     3841 aacttgtagg cgggctttat ggtttgtcca tgggatccat tttttttgga gagtcaatgt
     3901 ttacatttgc ctctaatgca tcaaaagccg catttttatc aatggcaagc tttgtgaaaa
     3961 agatggggtt ttcacttatc gactcacagg ttcatactcc acatgtggag actcttggtg
     4021 gcgagagcat aagccgccgt aaatacctcg agattcttaa gaacggctta caacaaaacg
     4081 atgttaccgg cagctggagc gaactctaca gctcggtaac atcatcaggt acaaccttag
     4141 ccaccgaaga tccaagatag acatcctttc cggattcagc ctcttcgtat ttactttccg
     4201 gcacaacctc gtaacgagta cgcggttcaa cctcacgtgg ctccggaacg ggttcagcct
     4261 caacagtctc ttttcgtatt ttttgcatca gctcctgcgc gcttgtctct ttccaacgag
     4321 tggcgatatg cctatgtttt ccgcgcggat acttctcaag atataattga aattcccttg
     4381 ccgcaagatc atatttcttt tgcataaagt aagcataacc cttcttaatt cgcgcatctt
     4441 catccttgtg atacaaaccg ttagacaatg tcttgttata atagccgatt gcccgcgaat
     4501 aacgtcccag agagtaatat gactccgcag tccaatataa ggcattttca ctatactttg
     4561 atgcaggaaa atttttaaga acaataccaa aatagttaat agctggccaa aaactcgcgc
     4621 ttcggtaact gctaagacct gcgttataag cctgatcaag atatgcccgt tttacatcag
     4681 gagtgtagct actaaaggca ccatgatgtg ccaagaagta atcatatttt ttaaaggctt
     4741 cgctgttttt acccaatcgc cttaacgatc ttgccttgcc caaaagagcc tcttcattat
     4801 catgattttc acgaagtgcc aagtcaaaag cattatcagc tttgtcaaca ttacccagcc
     4861 ttaaatgagt ataccctatc tctgaataga ttctgcttct aaactcgcta tccgtagaat
     4921 tgtttaaaac cgtatacagc tgattaagcc cctcttcaga ataattacgc tttattttca
     4981 aggcagccat acgggcaaga acctcgtttt ttttctcttt gtttgccatc acataggggt
     5041 tctttaaaag ataagaatat ttcagataag cgagcttgta aagatcgacc ttctcataag
     5101 actcggcaat tttaaaggta gagtctatcg cagctctagt ttcagggtaa cgcgccagaa
     5161 cacgagtata taaatcgata gcccgattaa tggactcacg atcattgcgc tcttgtataa
     5221 gactcgcctc cctgtacagc ttagcggcct c
//
LOCUS       DAOTGZ010000194         5227 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_3064
            NODE_18785_length_5227_cov_3.604408, whole genome shotgun sequence.
ACCESSION   DAOTGZ010000194 DAOTGZ010000000
VERSION     DAOTGZ010000194.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868088
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 5227)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5227)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:22:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,785
            CDSs (total)                      :: 2,751
            Genes (coding)                    :: 2,732
            CDSs (with protein)               :: 2,732
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 12 of 19
            Pseudo Genes (internal stop)      :: 5 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5227
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_18785_length_5227_cov_3.604408"
                     /isolate="AMR_MDS_3064"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>482
                     /locus_tag="P5123_11075"
     CDS             <1..>482
                     /locus_tag="P5123_11075"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRX16846.1"
                     /translation="ISDFMVTANASYIYSEIKVKDTDLVDYTNNERPLQGQSPYVLNL
                     GINYNNKRSGSSLGLLYNIYGRRIVRVGTTLNFSATDLNGDSYDYSLAEGDVYEEPIG
                     KLDFVFKQNIGKHGSLKFTAANLLDPEINQTQEKQNQSMDSETGRMSGGKTVKHTLLS
                     "
     assembly_gap    483..492
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            645..1847
                     /locus_tag="P5123_11080"
     CDS             645..1847
                     /locus_tag="P5123_11080"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRX16847.1"
                     /translation="MKKDLLKKSLAVFMATLMLLTFAACDDEDDDPKKKTEEPSGDYD
                     GQLKGDITSDYTIKADSTYEMVGTVRVKSGATLTIEEGVTLKANTSGALTYLIIEQGG
                     KINAVGTADKPITFTSDKEEGERSPSDWGGIAINGYATHCEGALTAEGEGDSGTYGGN
                     KDDDNSGTLKYVRIFFAGYAFDPETELNGLALQGVGSGTTIDYIQIHKGKDDGIEMFG
                     GKVDINHIVSTANGDDQIDATSGWRGAVKNAVAIGFSGDNCALEHDNNKGTPTATPRT
                     TITYENLIAVSVGSGDDVAAHIRRGTIADFVNCYFAGDDLANDIFFADGNDTEVSFDS
                     NTYFQNGGVATTDSPTINNSSSAATELDLAEFATLKAFNAAGGLDAIPASAMNVAWAK
                     GWTEFPEN"
     gene            2124..2489
                     /locus_tag="P5123_11085"
     CDS             2124..2489
                     /locus_tag="P5123_11085"
                     /inference="COORDINATES: protein motif:HMM:NF024845.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zf-TFIIB domain-containing protein"
                     /protein_id="HRX16848.1"
                     /translation="MQCAKCGSLMEKVIYESIEADRCLECKGLWLDAEVVNALIHMQG
                     SELVDSGDRSIGKLMNEKREIYCPLCGVLMNHLADPDNPDVEYEKCPACNGMFLDAGE
                     LSSLKGESFLNFVKKFKEN"
     gene            2491..3243
                     /gene="hisA"
                     /locus_tag="P5123_11090"
     CDS             2491..3243
                     /gene="hisA"
                     /locus_tag="P5123_11090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015842380.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003949 -
                     1-(5-phosphoribosyl)-5-[(5-
                     phosphoribosylamino)methylideneamino]imidazole-4-
                     carboxamide isomerase activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformimino-5-aminoimidazole
                     carboxamide ribotide isomerase"
                     /protein_id="HRX16849.1"
                     /translation="MRFRPCIDLHNGQVKQIVGSTLNREAKPVIENFVSSKDAVYYAN
                     MYKESNLTGGHIIKLGPGNDEAALLALKTYPGGMQLGGGVTAGNAVKYLDSGASHVIV
                     TSYVFIDGRINWENLDLLVKTVGKEKLVIDLSCRKKDDFYYVVTDKWQKFTSFTISEK
                     NLDMLSNYCDEFLVHAADVEGKCSGIETELVTMLGKWASIPVTYAGGVSSLGDLEEVK
                     EAGSDRIDITVGSALDIFGGKLPFADVVNFCS"
     gene            complement(3311..4093)
                     /locus_tag="P5123_11095"
     CDS             complement(3311..4093)
                     /locus_tag="P5123_11095"
                     /inference="COORDINATES: protein motif:HMM:NF012907.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl hydrolase family 18 protein"
                     /protein_id="HRX16850.1"
                     /translation="MTGYSITKNGSVRIVSSNFHNLCKKTIPATKFTPVITFDSAKSG
                     SIIFNNKKLRNQLVNSLSDFLNTNSFKTIQFDFEHLSPSNTGHYRSFLSELRKKNRYL
                     YISICLFPQVRWIDKYSKFTDISVLANSADEYMLMSYDQHGTTKKEGPVTSLSWAEEN
                     IRYIIKHIDAQKLILGVPLYGYSWDKNGKTKVITEKSFYRRFKPKEIVHLKEGISILD
                     KPKTYGYIYLSVGDSKTADYFLQLAKKYNLKGTAIWRLGFEK"
     gene            4510..5007
                     /locus_tag="P5123_11100"
     CDS             4510..5007
                     /locus_tag="P5123_11100"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRX16851.1"
                     /translation="MKTVLLVITFLTGLAAFMTDSPVFDGPKKGDRIALVSIYPDEQA
                     IMLPGELCYARVHYRAKEKQNVYVVTRVYSDRDSYRYDQISWTPATDYGNNEVAFSIP
                     QFTGPKGERIFAVDFALIEIDNDGREKVVSRTRKYEYVMGDIRNEKYYEQEDDGDGFI
                     EYALR"
BASE COUNT         1660 a          790 c         1108 g         1659 t
ORIGIN      
        1 atttcagatt tcatggttac tgctaatgct tcatatattt attcggaaat aaaggttaag
       61 gataccgatt tggtagatta tacaaataac gaacgtccct tgcagggaca gtctccatat
      121 gtgctgaatc ttggaattaa ctataataat aagcgtagtg gaagcagttt gggtttgctt
      181 tataatattt acggtaggcg cattgttcgt gtcggtacga cattaaactt tagtgctaca
      241 gatttgaatg gagatagtta tgactattca ttagcggaag gtgatgttta cgaagaaccg
      301 attggaaaac ttgattttgt atttaaacaa aatatcggca aacatgggtc actaaaattt
      361 actgccgcaa atctacttga tccggaaata aatcagacac aggagaaaca aaaccaaagt
      421 atggattcag aaactggtcg tatgagcgga ggtaaaactg ttaagcatac cttgctaagt
      481 tannnnnnnn nntaagttat agagagggac gaacatttag ccttagtctg ggatacagtt
      541 tttagaaatg aaattacgat tatatataac tgtctaataa taaagagtga tgattaatat
      601 aaaacaaggc tttaaaaaat tattaaataa taaagaggtt caaaatgaaa aaggacttac
      661 ttaaaaaatc attggctgta ttcatggcaa cgctaatgct tttaacattt gctgcttgtg
      721 atgatgagga tgatgatcca aaaaagaaaa cagaagaacc gtcaggagat tatgatggtc
      781 aattaaaagg tgacattact tcagattaca ctattaaagc tgattcaaca tatgagatgg
      841 ttggaacagt acgagtaaag agcggtgcta cactaacaat tgaagagggg gttaccctaa
      901 aggctaatac ttcaggtgca ttaacctatc taattattga acagggtggt aaaattaatg
      961 cagttggaac agctgataag ccgattacat ttacttcaga taaggaagag ggagagcgta
     1021 gcccgagtga ttggggtgga atcgcaatta acggatatgc cactcattgt gaaggggctt
     1081 tgactgctga aggtgaaggt gattccggaa cttatggagg taataaggat gatgataata
     1141 gcggtacatt gaaatatgta cgcatcttct ttgctggtta tgcttttgat cccgagaccg
     1201 agttgaatgg acttgctctt caaggtgttg gatctggtac aacaattgat tacatccaga
     1261 ttcacaaagg aaaggatgat ggaatcgaaa tgtttggtgg caaagttgat atcaatcata
     1321 tcgtttcgac tgcaaacggt gatgaccaga ttgacgcgac atcaggttgg agaggggcag
     1381 taaaaaacgc ggttgcgata ggttttagtg gtgacaactg cgctcttgag catgataaca
     1441 acaaaggtac gccaactgcg actcctcgta caactattac ttatgaaaac cttattgcgg
     1501 tatctgttgg atcaggtgat gacgtagcgg cacatattcg tcgtggaaca attgccgact
     1561 ttgttaactg ctatttcgca ggtgatgatc ttgcaaatga tatatttttt gcagatggta
     1621 atgatactga agtatcattt gattcgaaca cttacttcca gaatggagga gtggcaacta
     1681 cagattctcc gactatcaac aatagctctt cagcagctac agaacttgat cttgcagaat
     1741 ttgcgactct taaagctttt aatgcggcag gtggtcttga cgcaatacct gcatcggcca
     1801 tgaatgtcgc atgggctaag ggctggacag aatttccaga aaactaaaga tcatttgcaa
     1861 atgaaaggtg acaaatggcc ggagcttgat gtttcggcct ttttttgcta aatatatctt
     1921 attggttcga catatactgt tagtttcccc acttaaataa tctgcagatt gtacctgcaa
     1981 caaagtgtcc ccttttcctt atttcatgct ttacaaaatt ggtagcattc gtacgttaaa
     2041 gtatttgata ataatagttt gttataaata ggttcgaaca gatattaatg gtatattcca
     2101 ttagtaattt aataggagga gatatgcaat gtgccaaatg tggttctttg atggagaaag
     2161 ttatttatga gtctattgag gccgatagat gcttggaatg taaaggactt tggcttgatg
     2221 ctgaagttgt aaacgcattg attcatatgc aagggtcaga gcttgttgat tctggtgaca
     2281 gatcaatcgg taagttaatg aacgaaaagc gtgaaattta ttgcccctta tgtggtgtgt
     2341 tgatgaacca tttagcagat ccagataacc cagatgtcga atatgagaag tgtcctgcat
     2401 gcaatggaat gtttcttgac gcgggtgaat tatcatcgtt aaaaggagaa tcgtttttaa
     2461 actttgtaaa aaaatttaag gaaaattaat atgcgttttc gcccatgtat tgatttacat
     2521 aatggtcagg ttaaacagat tgtaggaagt actcttaata gggaagcaaa gcccgttatc
     2581 gaaaactttg tttcgagtaa ggatgccgta tactatgcta atatgtataa agagagtaat
     2641 ttaacaggtg ggcatataat caagctaggt cccggaaacg atgaagctgc cctgttggca
     2701 ttgaaaacct atccaggagg tatgcaacta ggaggtggtg taacggccgg aaatgccgtg
     2761 aaatatctgg attccggagc aagtcatgtt atcgttactt catatgtgtt tatagacgga
     2821 cgtatcaatt gggaaaattt ggatcttttg gttaaaacgg ttggcaaaga gaaacttgta
     2881 attgatctta gctgccgtaa aaaagatgac ttctattatg ttgttacaga taaatggcaa
     2941 aagtttacat cttttactat tagtgaaaaa aatttggata tgctttctaa ttattgtgat
     3001 gagtttcttg tacacgcggc agatgtcgaa ggtaagtgct cgggaattga aacagaactt
     3061 gtgacaatgt tggggaagtg ggcaagtatc ccggttactt atgctggtgg agtatcatcg
     3121 cttggtgatc tcgaagaggt aaaagaagca ggttctgacc gaattgatat tacggtagga
     3181 agtgccctcg atatttttgg aggaaaactc ccttttgccg atgttgttaa tttttgctca
     3241 tagataagat tgtttcgtaa tttattaatc ttaaggggtt tatagagtca ccatttgttt
     3301 gctggtttcg ttatttttca aatcccaatc tccagatggc tgtgccttta agattgtatt
     3361 tttttgcaag ttgtaaaaag taatcagccg tttttgagtc tcccacagat aggtagatgt
     3421 atccgtaagt tttaggctta tcaagaatcg agattccctc tttgaggtgt acaatttctt
     3481 tcggtttaaa tctcctatag aaagattttt ctgttataac ttttgttttc ccatttttgt
     3541 cccatgaata gccgtaaaga ggaaccccga gtatcaattt ttgagcatca atatgtttaa
     3601 ttatatatct aatattttct tctgcccaag ataaacttgt cacgggccct tcttttttgg
     3661 ttgtgccatg ctggtcgtag ctcataagca tatactcatc cgcactatta gccaatacag
     3721 aaatatctgt aaatttgctg tatttatcga tccatcttac ttgtggaaaa agacatatcg
     3781 aaatatataa atacctattt ttctttcgta gttccgaaag aaacgatctg tagtgacctg
     3841 tatttgaagg cgagagatgt tcaaaatcaa attgaatagt cttgaaacta tttgtattta
     3901 aaaaatcaga taatgagtta acaagttggt ttctgagctt tttgttgtta aaaataatgc
     3961 ttccggattt cgcagaatcg aaagtgataa ccggagtaaa cttagtagca ggaattgttt
     4021 ttttacaaag attgtggaaa ttagaggaaa caattctaac agagccattt tttgtaattg
     4081 aatagccagt cacataaaga acatcatatt ctaaactata gtttgttatg gtttttttag
     4141 aataatttgc atagtgaacg agatagcccc gtttttcggt ctgttctgct tctaaacaaa
     4201 aagaagattg tataattata ataaaaatta tataaaataa atttattttt attttgatga
     4261 tcaaactgat tttaacctca atttaggtgc gcccatttat ggagagcctt aaaacccaat
     4321 tttaagtgta aaactaattc tgtcagatgc aatataaaat cataataata aaaaaaattt
     4381 caaaaaatat gataactata tttttttggg aacaaaactg acgtctaaat cgtcttatat
     4441 atgagctctt aaaaaaaaac ggccgttttt aaagaaatta tagtaattta ctagatttag
     4501 gagaatttta tgaaaacggt tctattggta ataacttttt taacaggtct tgccgcattt
     4561 atgacagatt cgccggtttt tgatggtcct aaaaaagggg atcgtatagc tcttgtctcg
     4621 atatatccag atgaacaggc tatcatgctg cctggtgagc tctgttacgc aagagttcac
     4681 tatagggcta aagagaaaca aaatgtttat gtagtgacgc gtgtatattc agatagagat
     4741 agctatcggt atgatcagat atcatggact cctgcaacag attacggtaa taatgaagta
     4801 gctttttcga taccgcaatt tactgggcct aaaggcgaaa gaatttttgc cgtcgatttt
     4861 gcccttattg aaattgataa cgatgggaga gagaaggtcg tttcaagaac ccgtaaatac
     4921 gaatatgtta tgggtgatat acgaaacgaa aaatattacg aacaggaaga tgatggagat
     4981 ggctttattg aatatgcctt gcgatagatg tgttcctaat aaaatgggcg ccctcaaagc
     5041 gcccatgaag tcgataaatg tctatttttt tgaaacatta ttagggtttt tcttaatata
     5101 atcattatat gcgtctataa tacccatgat atcaatatct gaaagcggct tgtcgtaagc
     5161 ggctatcata ttatcacatt gaatttcgtc tttgtaatct tcaaagtcgt atgcactgat
     5221 aaaaacg
//
LOCUS       DAOTGZ010000195         5199 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_3064
            NODE_18908_length_5199_cov_5.706260, whole genome shotgun sequence.
ACCESSION   DAOTGZ010000195 DAOTGZ010000000
VERSION     DAOTGZ010000195.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868088
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 5199)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5199)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:22:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,785
            CDSs (total)                      :: 2,751
            Genes (coding)                    :: 2,732
            CDSs (with protein)               :: 2,732
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 12 of 19
            Pseudo Genes (internal stop)      :: 5 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5199
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_18908_length_5199_cov_5.706260"
                     /isolate="AMR_MDS_3064"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..592
                     /locus_tag="P5123_11105"
     CDS             <1..592
                     /locus_tag="P5123_11105"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRX16852.1"
                     /translation="DTGLNLTMEPVFDRVTIFGVDAAYAMGKLNTRFEGIYSPDMIAS
                     EKIDDQQKAAAMAQIMGGSDSAPLQDGTRRNYYSYGVGADYNLWGNYGTVYCEWMQAR
                     FVDESDIEKELQSDILALRIEDSFFSQALNLSAGSMIRIENMSAGYALTAEAEYDFKD
                     GLTATAGAYYFVPNDDSYMKLFEDMSMIYLRMKFNF"
     gene            689..1783
                     /locus_tag="P5123_11110"
     CDS             689..1783
                     /locus_tag="P5123_11110"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRX16853.1"
                     /translation="MSNPFSFSGTVADRGFCNRKQEQKDIIDFINNSENVLLHANRRM
                     GKTSLIYKIFSKLDKNITHLYIDLYGTVSENDFIEALMKNAGQLESRSEKLLKTAKEI
                     FRSSNVSLSIDPVTNLPSLTPVFGSSKKSLLLENAMLLLERYSEKKRMVIVFDEFQEI
                     ENYSGGEFEKRLRGFIQRHDRISYIFAGSRQHILAGMFNSKGKAFYKLAHSYPLSEIE
                     EDDYIKWIKRLFANKKKISDNLIIEVVNLCERHPMYIQQFFYHLWDADLKNPDIIDQT
                     LDLILLRNRNEYMNIWDSLTLNQRKTLKLIAANEGKELYSADSLKSVSLTTPSQVTKT
                     LQSLTQKDIIQKNGVYKIQDVMFKQWILRL"
     gene            1991..3139
                     /locus_tag="P5123_11115"
     CDS             1991..3139
                     /locus_tag="P5123_11115"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF021127.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042578 - phosphoric ester hydrolase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0045227 - capsule polysaccharide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CapA family protein"
                     /protein_id="HRX16854.1"
                     /translation="MKIKSIILAILLSLLVNSCSSAQLSSLFGSGDKNSFSLVAVGDI
                     CHSHTSDYVAEFRALGKKKYIPTRKIISSADLSFANLETPYTTREPVLEKQFAFASDP
                     EELDHILWAGFNLLSLANNHSHDAGQAGIVDTIELLQKKKRSNRQVREPLTWAGTGRT
                     FSEAREPKFIKLPGKNFKIAFLAYGNNSHSLVNTFEVQRAANEIQKIKRSDPNTIIIV
                     SVHSGTEYSHVPGSATISYYRTLIDAGAQVILGHHPHVLQGIEQYRNGIIFYSLGNFS
                     FGSYTSRHIENDAKLYSMIAKMDFRRINSTIDIRLVIYPIYVDNVYPMIVNNRKLEPS
                     PFVPQILQKPFSKPILDSIISWSNQIPGNDIRLSSDETTLNTRIRIDL"
     gene            3266..4324
                     /locus_tag="P5123_11120"
     CDS             3266..4324
                     /locus_tag="P5123_11120"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRX16855.1"
                     /translation="MDKKVFRRTDGMVLILPLIAVCLGFAVYIFISVAKLDPKTTKFL
                     WAAIALPLILPIFLQIFHTITIDDTYITKGLRRKKIKISEIIRFGEGKLTGYLDNRLR
                     SSSTKITTSHFKILDSSGRTMRINWMGYKDKHDLYKLLVDKIGCPPETLYTDGIYGIY
                     FIPLEKCLNCGQENNGKLVKCTYCGRFLKPAPEYDKHLEKLLYPGKDYQPYLTKGFSF
                     KNTTSGKIAGSILTFYALLAAAASNLHFTDNLALYIAPFPYPIHGYIAAFFSIWGFYF
                     LLRHNSKIAIPLLFLCAIILGTQPWVSSLIRSRLPLDAYVEEIAAMLPTYHFIISGTA
                     LATFALLHQKIPFFKIKK"
     gene            4645..>5199
                     /locus_tag="P5123_11125"
     CDS             4645..>5199
                     /locus_tag="P5123_11125"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRX16856.1"
                     /translation="MKDILQKASIRRKLTTLNILLLLPVSIGIGAFAYYQAYNAAYKQ
                     LIDNAPITARYSANQLSNKLETYMIAIEGITKRQEIRSMEFEQQQPVMRQETARLRLK
                     DMSIVYPDGSAIMTSSKTDTFANKDFIKEAFKGKTVHSDIEINSADNSITMYIASPIR
                     GNDNRTAAVLTAKVDAEWLSLAADN"
BASE COUNT         1685 a         1085 c          949 g         1480 t
ORIGIN      
        1 ggacaccgga cttaatctga ctatggaacc cgtttttgat cgcgttacaa tttttggagt
       61 cgatgccgca tacgcaatgg gaaaacttaa tacccgattc gaagggatat actccccgga
      121 catgatcgct tcagaaaaaa ttgacgatca gcaaaaggct gctgcaatgg cacaaataat
      181 gggtggcagt gactccgcgc ctcttcaaga cggaacaagg cggaattact atagttacgg
      241 agtgggcgca gactacaatc tttggggcaa ttatggaaca gtctactgcg aatggatgca
      301 ggctcgtttt gttgacgaaa gcgatataga aaaggagttg caaagcgata ttctggcatt
      361 aaggatcgag gactcctttt tctctcaggc gttgaatctt tccgcaggat caatgatcag
      421 gatagagaac atgtccgcgg gatatgccct taccgccgaa gccgaatatg acttcaaaga
      481 cgggcttacc gcgacagccg gagcatacta ttttgttccc aacgacgaca gctatatgaa
      541 gcttttcgaa gatatgagta tgatatatct tcgaatgaaa tttaattttt gatataggga
      601 gaataccctc tactttttaa aaaaattgtt gctattacac ctcaagtgta gtattttaca
      661 cttaatacaa taacacttga ggtgtaatat gagcaacccg ttcagttttt ccggcactgt
      721 cgcagaccga ggtttttgca accgcaaaca agagcaaaaa gacattattg attttatcaa
      781 taattccgag aacgtactgc tgcatgcaaa tcgcaggatg ggcaaaacat cactgatata
      841 caaaatattt tctaaactcg ataaaaatat aacacatctg tatatcgatc tgtatggaac
      901 tgttagcgaa aatgatttta ttgaagcatt gatgaaaaat gccggtcaac tggagtccag
      961 atctgaaaag ctgttgaaaa cagctaaaga gatatttaga tcgtccaatg tttcactttc
     1021 aatagatcct gttacaaacc ttccctcgtt aacacctgtt ttcggttcat caaaaaagag
     1081 tcttctactt gaaaacgcaa tgcttctact tgagcgttat tcagaaaaaa aacgaatggt
     1141 aatagtcttt gatgagtttc aggagattga gaactattcc gggggcgagt ttgaaaaaag
     1201 gctgcgggga tttattcaaa gacacgacag gatatcatat attttcgcgg gaagccgtca
     1261 acacatcctt gccggtatgt tcaattcaaa gggaaaagct ttttacaaac tggctcacag
     1321 ttatccttta tcagaaattg aagaggatga ttatattaag tggataaaaa ggttgtttgc
     1381 caataaaaag aagatatccg acaatcttat aatagaggtc gtcaatcttt gcgaaagaca
     1441 tcccatgtac attcaacagt ttttttatca tctctgggac gcggatctca agaatcctga
     1501 tataatcgat caaacacttg accttatact gctacgtaat cgaaacgaat atatgaacat
     1561 ctgggacagt ctgacactta atcagcgtaa gacccttaaa ttgatcgcgg caaatgaagg
     1621 caaagaactc tactcggcag actctctgaa atctgtaagt ctgacaacac catcacaggt
     1681 tacaaagaca ttgcaatctt tgacacaaaa ggatataatt cagaaaaacg gtgtttacaa
     1741 aatacaggat gtaatgttta agcaatggat tttaaggtta tgatctgtag attatttgca
     1801 ggttggctat gacggttaaa gggaatttat cgtgttaccc gtgaagactt caccttgtgc
     1861 tcttataaac acacctacaa gagatataca cttttttgtt tgacaccaaa tatataatct
     1921 cagttattat ggtctttcgg attatcttgt aatcactaat acaatcatca actacaaagg
     1981 ctctagcaaa atgaagataa aatcaatcat actcgccatt ctgctgtcac tgcttgttaa
     2041 ctcgtgtagc tcggcacagc tttcatcact tttcggatca ggcgacaaaa actcatttag
     2101 ccttgtagct gttggcgaca tatgccattc gcatactagc gattacgttg ccgaatttag
     2161 agccttaggc aaaaaaaaat atatacccac acgcaaaata atatcatctg ccgacctatc
     2221 tttcgcgaac cttgaaactc cctatacaac gcgcgaaccc gtacttgaaa aacagtttgc
     2281 gtttgcgtca gatccggagg agctagacca tatcctctgg gctgggttta accttttaag
     2341 cctggctaat aatcactcac atgacgcagg ccaggcaggc atcgttgaca caatcgagct
     2401 actccaaaag aaaaagagat ctaacagaca agtccgcgaa cctttaacct gggctggcac
     2461 aggccgaacc tttagtgaag ccagagaacc aaagtttata aaactgcctg gtaaaaattt
     2521 taaaattgcg tttcttgcat atggaaacaa cagccattcg cttgttaaca catttgaagt
     2581 gcaacgagct gcaaacgaga ttcaaaagat taaacgatcc gatccaaaca ctataataat
     2641 cgtctcggtg cactccggta cagaatatag ccatgttccc ggatccgcca ctatatctta
     2701 ttatcgaaca cttatcgacg ctggtgcgca agttatactg ggccaccacc cccacgttct
     2761 tcagggaatc gagcagtacc gtaacggcat tatattttat tcgcttggaa atttttcttt
     2821 tggaagctac accagtcgcc atattgaaaa tgacgcaaag ttatacagca tgattgccaa
     2881 aatggatttt agacgcatca actcgaccat agatatccgt cttgttattt accctatata
     2941 tgtggataat gtttatccaa tgattgtcaa caatcgtaaa cttgagcctt ccccatttgt
     3001 tcctcaaatt ttacaaaaac ccttttccaa acctatactc gattctataa ttagctggtc
     3061 aaaccagatc cccggaaacg acatacggct ttcatccgac gaaacaaccc tgaacaccag
     3121 aatacgcatt gacctttaaa tttcttttta gcaaaaagca tttttcttct ttcatgttga
     3181 tttttaccag tcacagatta acttatatta tataacccga taacatcact gctctattca
     3241 aaaaaaattt tgacggagta atattatgga taaaaaagtc ttccgaagaa cagacggtat
     3301 ggtactgata cttcctctta ttgcagtctg tctcggattt gctgtgtaca ttttcatatc
     3361 tgttgcaaaa cttgatccaa aaacaacaaa atttttatgg gctgctatcg cgttaccctt
     3421 aatacttccc atatttttac agatttttca tacaataaca attgacgata cctatataac
     3481 aaaaggctta cgacgtaaaa aaataaagat ttcggaaata atcagatttg gcgaaggcaa
     3541 actaacaggg tatcttgaca atagactcag atcgtcttct accaagatca caaccagcca
     3601 cttcaagatt cttgactcat ccggacgaac catgcgaata aactggatgg gttataaaga
     3661 caaacacgac ttgtacaaat tgcttgttga taaaataggc tgtcctccag aaacgctgta
     3721 caccgacgga atttacggaa tatattttat tcctcttgaa aaatgcttaa actgcgggca
     3781 agaaaacaac ggcaagcttg taaaatgtac ctattgcgga agatttctga aacctgcccc
     3841 tgaatacgac aaacatcttg aaaagcttct ttatccagga aaagattacc aaccttacct
     3901 cacaaaaggt ttttcgttta aaaacacaac atctggcaag attgctggct caattctaac
     3961 tttttatgca ctgcttgcag ctgcagcgtc taacctgcat tttacagata atctcgcgct
     4021 atatatagca cctttcccct acccgattca cggttacatt gccgcatttt tttcgatatg
     4081 gggtttttac tttttgctaa gacataattc taaaatagca ataccccttc tttttttatg
     4141 cgccattata ttgggcacgc aaccatgggt gtcatcatta ataaggtctc gactccctct
     4201 ggatgcttac gttgaagaaa tagctgccat gctaccaaca tatcatttta tcatttccgg
     4261 tacagctctt gctacttttg cgctacttca tcaaaaaatt ccttttttca aaattaagaa
     4321 atagttgtat attcttaaca aacaaagcag ccaaaagaaa gagccaaaaa aagtttatgt
     4381 atcacaggca atgatttgtt ttctgttttt tacttgtctt ttttacacgg catatcgata
     4441 attaacgtta tcagaagctg ttaaatttat ttgagatcac cttaaattaa tccgcaaatt
     4501 atattttaac catatacgac tcgtgcaatt aaataagcat aatcaggctg tttaaacagt
     4561 ttatatatca tttagcaaat agtcataata tagttgtata ttcccgataa caatgctata
     4621 ttcggtacca atagggataa ttgtatgaaa gatatactgc aaaaggcatc tatcagacga
     4681 aagcttacga ctcttaacat attgctgctg ctaccggtat ctattggcat aggggccttc
     4741 gcctattatc aggcatataa cgccgcctac aagcagctta tcgataatgc tccgattact
     4801 gcccgttaca gtgccaacca gctatccaat aaacttgaaa catatatgat tgctattgaa
     4861 ggcataacga agagacaaga gattcgctcc atggagtttg agcagcaaca gcccgtaatg
     4921 agacaagaga ctgcccgatt aagattaaaa gatatgagca tagtttatcc agatggcagc
     4981 gccataatga caagcagcaa aaccgataca tttgcaaata aagacttcat aaaagaggct
     5041 ttcaagggga aaacagtaca ttcagatatc gaaataaatt ctgcggataa tagcattacc
     5101 atgtatatcg cttcaccaat aaggggcaac gacaaccgaa cagcagccgt attgacggca
     5161 aaggttgatg ccgaatggtt atcacttgct gcggacaac
//
LOCUS       DAOTGZ010000196         5171 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_3064
            NODE_19038_length_5171_cov_3.892690, whole genome shotgun sequence.
ACCESSION   DAOTGZ010000196 DAOTGZ010000000
VERSION     DAOTGZ010000196.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868088
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 5171)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5171)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:22:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,785
            CDSs (total)                      :: 2,751
            Genes (coding)                    :: 2,732
            CDSs (with protein)               :: 2,732
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 12 of 19
            Pseudo Genes (internal stop)      :: 5 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5171
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_19038_length_5171_cov_3.892690"
                     /isolate="AMR_MDS_3064"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..384)
                     /locus_tag="P5123_11130"
     CDS             complement(<1..384)
                     /locus_tag="P5123_11130"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRX16857.1"
                     /translation="MYKKTLFVFLLFLWSSLYANQSKYDPTIILSSGFGARYGASSLG
                     VGTDYYPLNYSNHHLGLICGIGYRYDLFMWTIGSRYAYGNRDRIIVDILYGVMDYDVE
                     MHEDGSYHNAKSFTNPSLLIGYQRLT"
     gene            complement(430..885)
                     /locus_tag="P5123_11135"
     CDS             complement(430..885)
                     /locus_tag="P5123_11135"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRX16858.1"
                     /translation="MSTRSDRGDDAENSGYNKDFTFMSEEAYEKYESKIRDLCDEYML
                     EYEDEEFVELINHDAVRSSGSTHLYARARRSGSYLLGYAYKAESWITGNTPKEMHLVM
                     DINGYSTAVKTVKGKRSISLIKGKGLKRTMYTHHETMSPYIYIKLKVKK"
     gene            complement(1553..2665)
                     /locus_tag="P5123_11140"
     CDS             complement(1553..2665)
                     /locus_tag="P5123_11140"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRX16859.1"
                     /translation="MDEIYSIREISRITCVVHQRLSARLADFLRVSGVNTVFARNGRS
                     VRHFVKKRPFFIPGSSVSLQDSSSDIFQFTLPRKFCENMMSGVVEVCGLEEGGRGTVY
                     SQDMVMYGGHEPNIPDIKEYVEPHFHDDLACITAILSMRGSADVCAGLALELGTCVPV
                     ITHGHGTGMRDKLGLLRITIPPEKELITLLAPQHDADGIIRMLVEGAKLDKPGRGIIY
                     RTTVASGLTDTRIHIGHQKYAASMEQIIAALDKLHFGTSWRHRFADNENLRWSHQLVS
                     GNKEVNVICPEGVVKNLTGIAMENGAGGATTSTLRRTAISDNVPGAAAYEQSTLIIDS
                     QKADSVIKALLASKEFKEAAGSFITITDAPAAFTYR"
     gene            complement(2658..4622)
                     /locus_tag="P5123_11145"
     CDS             complement(2658..4622)
                     /locus_tag="P5123_11145"
                     /inference="COORDINATES: protein motif:HMM:NF019194.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1538 family protein"
                     /protein_id="HRX16860.1"
                     /translation="MARSKGISIPLRSAFYLVVTHFAGLIKDQVKSVAFIILYLVLFQ
                     IIVLDSPLQDAAGVATGVALVIAGLAFFLEGIMLGLMPLGERVGVKITGRGGIASALV
                     FALLIGLGSTFAEPAIAALRTAGSSVTPWDAPLLFVLLGRHADALFTAIGIGVGAAVV
                     LGMLRLYYGLSLKPFLFVSVSAALAISLAMLYDPNLKSVVGLAWDTGAVTTGAVTVPI
                     VLALGIGVSRSSGNTEKAGAGFGTVALASVLPVVTVLLLAVILNSKVPVPTSETEFYS
                     AENRETAMMLFINSEEFYKHGLGSSDLEIRKQLRAEFEDLYNNKNLENNIRDSEFSGE
                     TIRDILKVESFLSVRAILPLTMLLAIALIFVRARPENTDEVVLGVVLAIVGMAILSAG
                     IRLGLAPLGDETGGQLPRTFRTEPEDIGRVVFENFDDKLLFESIDVTGERKQFFYSHY
                     DSEPRAIEFDPNKYNSINKRYVHIVSRPPLFGPDLTLLGIFLVFAFAFGMGWGSTLAE
                     PSLNALGKTVESITVGTVKQKGVVYAVSLGVGLGLVVGVARIMYDVSILWLIVPPYLL
                     LLGLTFFSDEDFTGIAWDCGGVTTGTVTVPLVLAMGLGIGNELDVIDGFGILSMASVY
                     PILSVMIYGLVLKSRQQRTLIAKPGEENNG"
     gene            complement(4796..>5171)
                     /locus_tag="P5123_11150"
     CDS             complement(4796..>5171)
                     /locus_tag="P5123_11150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012059930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="fibronectin type III-like domain-contianing
                     protein"
                     /protein_id="HRX16861.1"
                     /translation="PYGFGLSYTKFDFKDASFNKEDEKIIASCTVSNSGSCEGTEVVQ
                     MYVGFKKSAVDRPVRLLRAFERVSLQSGESRNIKLGCDLSKLCYYDEKSSQMFFEKME
                     YEVYIGNCCSDKHLLAGTVDLS"
BASE COUNT         1693 a         1161 c          858 g         1459 t
ORIGIN      
        1 cgtaagtcgt tgataaccta ttaacaagga tggatttgta aatgattttg cattgtgata
       61 cgacccatcc tcatgcattt ctacatcata atccatgaca ccataaagaa tatctacgat
      121 aatacgatcc cgatttccat aagcatatcg agaaccgatt gtccacataa aaaggtcata
      181 tctgtatcca ataccacata ttaaaccaag atgatgatta ctataattta atggataata
      241 atcagttccg acacctaacg acgaagcgcc atagcgagca ccgaaacctg acgacaaaat
      301 aattgttgga tcatattttg actgatttgc atacaaagaa gaccataaaa atagaagaaa
      361 aacaaataga gtttttttat acatcaattt tccttatttg tagccgatta aaatcggcta
      421 caaaacattt tactttttaa cttttaattt gatataaata tatggagaca tagtttcatg
      481 atgagtatac atagttcgtt ttaatccttt accttttatt aacgatatgc ttcgcttacc
      541 ttttacagtt ttaactgcag tactatatcc attaatatcc ataaccaagt gcatttcctt
      601 tggagtattc cccgtaatcc atgattcagc tttgtatgca tatcccaaca aataactacc
      661 cgatctccta gcccgtgcgt acagatgtgt tgatccagag gatctaaccg catcatgatt
      721 aattaattct acgaattctt catcttcata ttcaagcata tattcatcac ataaatctct
      781 aatttttgat tcatattttt cgtatgcctc ttctgacata aatgtgaaat ctttattata
      841 acctgaattc tctgcgtcat caccacgatc tgatcgagta ctcactgata catcatcaaa
      901 ctcaatttca ttacaatctg aaagctcatt aattattgat tgttgctttt gatttatttc
      961 acttatctgt ttttttgaca aagttggttc ttggtcagtg ctatgttcta tgccacttgc
     1021 atcacaaccc caaaaaccag caattaaaaa ggaaagaaaa ataatgcggg ggattgttct
     1081 aaaactgaga aactgagaaa ctgagaaact gagaaactga gaaactgcct tttcataata
     1141 tttactcctt tattttttta ttaaggtatt gattatatca cattataaaa agtcaataat
     1201 aaaattaaga tatatgtaat tttacacatt actatctttt ctaattatta tttgtcgaaa
     1261 tacaagccca aatgtacttt ttttaatgaa attaataaac catagaaata tctacctaaa
     1321 ataaatatga ataactgccc aataatattt tggtttttat aatttaagta aagattgtct
     1381 ttaaatacat agttttatcc tctgaaaaat aattaacagg aactttagag aaatacctat
     1441 aaattggttt gcaaacaatg ataccctaaa aaatattttt gaagttgtct agtttgctat
     1501 aatttgaagt tttaaattat ctttaactac atcgtatttg ataataacaa aatcatctat
     1561 aggtaaacgc cgccggcgca tcagtaatag taataaacga tccggcagcc tccttaaatt
     1621 cttttgaagc aagaagtgcc ttaatcacag agtcggcctt ttgggaatca attataaggg
     1681 tgctctgttc ataggcagca gcgcctggta cgttatccga tatggcagtc cggcgcagag
     1741 ttgaagttgt agcccctccg gcaccatttt ccattgcaat tccagtaaga tttttcacca
     1801 ctccttccgg acaaataacg ttaacctctt tattaccaga aactaattga tgcgaccatc
     1861 tcaggttttc attatccgcg aatcgatgtc gccatgatgt tccgaaatgc agtttgtcaa
     1921 gagcagcaat aatctgctcc atactagctg catatttttg atgccctatg tgtatccgtg
     1981 tatcagtaag tcccgaggcg acagtggttc tataaataat accccgtcca ggtttatcaa
     2041 gttttgcacc ttcaaccaac atacgaatta taccatccgc atcatgctgt ggagctagaa
     2101 gagtaattag ctctttttca ggaggaattg taatgcgtaa tagtcccagt ttatctcgca
     2161 tacctgttcc atgaccgtga gttattaccg gcacacaggt tccaagctcc agagcaagtc
     2221 ccgcacagac atctgcgctg ccccgcattg ataaaatagc agtaatgcat gcaagatcat
     2281 catgaaaatg aggctccaca tactctttta tatccggtat gttaggttca tgaccgccat
     2341 acatgaccat atcttgtgaa taaaccgttc cccgcccccc ctcttccaat ccacacacct
     2401 ctacaactcc ggacatcatg ttttcacaaa attttcgtgg caaggtaaac tgaaatatat
     2461 cagatgacga atcctgaagg ctcacggatg atcctggtat aaagaaaggg cgcttcttta
     2521 caaaatgtcg aacacttcta ccgttgcggg caaaaacggt attcactccg gacacacgta
     2581 aaaaatcggc aagtctggca cttaaacgct ggtgcacaac acaggttata cggctaattt
     2641 ctcgtattga atatatctca tccattattt tcttctccgg gctttgcgat cagtgttcgc
     2701 tgttgacgtg actttagaac aagtccgtaa atcattaccg aaagaatcgg ataaacagaa
     2761 gccatagaaa gtataccgaa accgtcaata acatcaagct cgttgccgat acctaacccc
     2821 atcgccagga caagtggaac cgttactgtc ccggttgtta caccgccaca gtcccatgcg
     2881 ataccagtaa aatcttcatc actaaaaaag gttaacccga gaagaagcaa atatggtgga
     2941 actataagcc agaggattga gacatcgtac attatcctgg caacacccac aacaaggcca
     3001 agccccaccc caagcgaaac cgcatataca actccttttt gtttaacagt accaactgtt
     3061 atactttcta ccgtttttcc aagagcgtta agtgatggtt cagcaagggt ggacccccac
     3121 cccataccga aggcaaaagc aaaaaccaga aatattccaa gcaatgttaa atctggacca
     3181 aatagcggcg gacggctgac tatatgcacg taacgtttat ttatagaatt atacttatta
     3241 ggatcaaatt cgatagcacg tggttcagaa tcgtaatgcg aatagaaaaa ctgtttacgc
     3301 tcacccgtaa catctatgct ctcaaaaagc aatttatcat caaaattttc aaaaaccaca
     3361 cggcctatat cttcaggctc tgttcggaag gttctcggca actggccacc cgtttcgtca
     3421 cctaacggtg caagcccaag tcttatacct gctgataaaa tagccatacc tactattgca
     3481 agaacaactc ccaatacaac ctcatccgtg ttttccggac gggctcttac gaatataaga
     3541 gcaatggcga gaagcattgt taaaggcaga attgctctca cagaaaggaa agactcgacc
     3601 ttcaatatat ctcttatagt ctcacctgaa aattcagaat ctcttatatt attttccagg
     3661 tttttattat tatacaaatc ttcaaactcg gctctaagct gtttgcgaat ctccaaatct
     3721 gaagaaccaa ggccatgttt gtaaaactcc tcactattga taaaaagcat catggcggtt
     3781 tcccgatttt cagcagaata aaactcggtt tcacttgtag gcacgggaac cttgctgttt
     3841 aaaataacag caagcaacaa gacggttaca accggaagaa cggatgcaag agcaacagtt
     3901 ccaaaaccgg ctccagcctt ttctgtattt cctgatgagc gggaaacacc gataccaaga
     3961 gccaacacaa taggtactgt cacggctcca gtagttacag cacccgtatc ccaagcaagc
     4021 ccaaccacac ttttaagatt agggtcataa agcattgcaa gtgagatggc caaggccgca
     4081 ctgaccgaaa caaacagaaa aggttttagg gataacccgt aatatagtcg cagcattcca
     4141 agaactactg cagcaccaac cccaatacct attgctgtaa aaagagcatc agcatgacgc
     4201 cccaagagca caaacagaag gggtgcatcc caaggagtaa cagaagatcc cgcggtacgc
     4261 aaagcagcta tggccggctc agcaaatgtt gaccccaaac cgattaacaa agcgaaaaca
     4321 agtgcagaag caataccgcc acggccagta atcttaacgc cgactctttc tccaagaggc
     4381 ataagtccga gcattattcc ttcaagaaaa aatgcaagtc ctgcaataac aagggcaacg
     4441 cccgttgcaa ctccagccgc gtcttgtaac ggcgaatcga gcacaataat ctgaaaaagt
     4501 acaagataca aaataataaa cgcgacggat ttcacctgat ctttaattaa accggcaaaa
     4561 tgagtcacaa caagataaaa cgcactgcgt aaaggtatgc taatgccttt ggatcgcgcc
     4621 atagaaatac catctttaaa aattattctt tatagtatcc acaaacggaa atgtgtccac
     4681 aacaaaacca ttaaaaaata ttacagtcga ccaaatagta ctctttagta tccactcgca
     4741 aaaaaaaccg accgtattga ccggttaata ttttttttag ttaaaacgat ctcatttaag
     4801 agagatccac agttcccgct agcagatgtt tatcactaca gcaattaccg atatatactt
     4861 catattccat tttttcaaaa aacatttgcg aacttttctc gtcatagtaa catagcttac
     4921 tcagatcaca gccaagtttt atattccgtg attcacccga ttgaagagag acacgttcaa
     4981 acgctcttag cagacgtacc ggtctgtcaa ctgcagattt tttgaatcca acatacattt
     5041 gaacaacctc ggttccctca caactgccgc tattcgatac tgtacaggaa gctattattt
     5101 tttcatcctc tttattgaaa ctggcatctt taaaatcaaa tttggtatat gacaatccaa
     5161 atccgtaagg c
//
LOCUS       DAOTGZ010000197         5134 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_3064
            NODE_19232_length_5134_cov_7.525694, whole genome shotgun sequence.
ACCESSION   DAOTGZ010000197 DAOTGZ010000000
VERSION     DAOTGZ010000197.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868088
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 5134)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5134)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:22:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,785
            CDSs (total)                      :: 2,751
            Genes (coding)                    :: 2,732
            CDSs (with protein)               :: 2,732
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 12 of 19
            Pseudo Genes (internal stop)      :: 5 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5134
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_19232_length_5134_cov_7.525694"
                     /isolate="AMR_MDS_3064"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1778
                     /locus_tag="P5123_11155"
     CDS             <1..1778
                     /locus_tag="P5123_11155"
                     /inference="COORDINATES: protein motif:HMM:NF014713.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0017004 - cytochrome complex assembly
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="cytochrome c biogenesis protein CcdA"
                     /protein_id="HRX16862.1"
                     /translation="YNEEDSFIKVQLDIDSKGYIYANPKGPGTGKAIQISLQHDLTPV
                     STLLYSQPSVKYQPAVGEWVNIYRDQARFYILVPEILKKEDNTLSITVDALFCDDISC
                     TPFSKTVTTTLNMGEDSRIADNSIPPDFTQSDIPQDPDTNESVTTDKPSSLIPQIDKP
                     WFIERSSVTTVLGAIITGLLAGLFLNIMPCVLPVISLKLMYLIKNSGKSRSMIITNSI
                     FYSLGIIFTFVILASLAAFAGHNWGSLFQNTAFIRVMIVIIFTMALSLFGVFTFSTPP
                     ILSSLSGKFSGNITSALFNGFLATVLATPCSGPFLGATLAWTLTQPPLIIYAVFLSIG
                     TGLALPFFVLTLFPKLISYIPKSGSWSLYSERLFGFILAASAVYFLSILESRALIGTL
                     ILIWFVSIGLWQLGQFATPVSSKKSRTISYIILLFLVLAGYRLSVYYSKPVNSSIGTI
                     DYSHSLLNELNNNETIVAVKFTADWCSNCKYVEMTVFNKDSVKQEFEKKGLSLLKADI
                     TAQGTQAEELLSALGSRSIPFLALFYPGEGRQNPLCLDDIYSENDLLKIIEIATRQAD
                     TQNNSEKEEKADKPEKIEALTEIAP"
     gene            1964..3247
                     /locus_tag="P5123_11160"
     CDS             1964..3247
                     /locus_tag="P5123_11160"
                     /EC_number="6.3.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017854215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004019 - adenylosuccinate synthase
                     activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006164 - purine nucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate synthase"
                     /protein_id="HRX16863.1"
                     /translation="MSCSVVIGAQWGDEGKAKMIDFFSQNADIIVRYQGGANAGHTVV
                     ANGEKHVFHLIPSGILHPGKICVLGNGVVLDPMQLQKEMDNLTAQSIDINYRLLISDA
                     AHLILPYHKALDSALEELRSNKIGTTGRGIGPCYADKCLRTGIRVGDIFLDEKTLEKK
                     VRQTLEVKNLQLVKMHKKDPFSIEEIMDIILDFKKHTQKMIINTGYYLNEEAKKNKTI
                     ILEGAQGFGLDIDHGTYPFVTSSNPTIGGALLGSGLNHSVIDKVIGISKAYITRVGEG
                     PFPTEDFGDDGNTLRDKGGEYGSTTGRPRRCGWFDVELMKQTARICGLTDITLTKIDV
                     LSGFEKVKVAVGYEYKGKKIDMFPSYLLDDIKPIYEELDGWTEDISECKSYEDLPQNT
                     RDYIDFLEKSVGVPMSIISVGPDRENTIVRDIWQQ"
     gene            complement(3368..>5134)
                     /locus_tag="P5123_11165"
     CDS             complement(3368..>5134)
                     /locus_tag="P5123_11165"
                     /inference="COORDINATES: protein motif:HMM:NF013253.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-hydroxy-acid oxidizing protein"
                     /protein_id="HRX16864.1"
                     /translation="ARGVRCVNNKNEISDAFHAAKSASPSGELIMEEYMEGPELSIDA
                     VVFDGEVTFTGIADRIIEDFPYFIEKGHTMPSQLDSEIIEESKRVMKLGIKALGINHG
                     YAKGDIKVTPDGVKIGELAARLSGGFMSTHTFPYSTGVDLMKAAIEIALGQEPGNLEP
                     VISKVSIERAIITEPGIIKSIDNLQEATAIKGINDIFINVVPGDRVVKPKSNVEKAGH
                     IIATANTLEEAEEAVKKARNTISIKIASDNEVSMDIIRAKAREKFGKACICCKDCNGE
                     NCTSSIPGMGAVGTGESFKRNISSLAEYKIITRVIHDVNNPDMSIGLFNKKMDMPVFA
                     SPITGTVTNMNGAIDEFLYNKAVVKGSAQCGIVAMVGDGASPEKYRVGLNAIKEFGGG
                     IPIFKPRKEKKELLTRIKEAEDAGAIAIGVDIDAIVLKTMNLRNQATGARGVDKLKEI
                     ITNSKLPFVLKGVMSVEDAKDAVATGAAGIVVSNHGGRVLDQMPGIMDVLPEICREVK
                     GKITIIADGGFRSGIDVFKALAVGADAVSIGRPICVAAVGMEEDGVTYYLNTIKEEFM
                     KCMILTGCQKVEDINENCIRKC"
BASE COUNT         1496 a         1111 c          940 g         1587 t
ORIGIN      
        1 tatacaacga agaagactca tttatcaagg ttcagcttga tattgactca aaaggctata
       61 tctacgcaaa ccccaagggg cccggaacag gaaaagccat tcaaatttct ttacagcacg
      121 atttaactcc ggtatctact ctgctttaca gccagccttc tgttaaatac cagcctgccg
      181 ttggagagtg ggtgaacatt tacagagacc aagctcgctt ttacattctt gttccagaaa
      241 tccttaaaaa agaagacaat accctgtcaa taacagttga tgctcttttt tgtgacgata
      301 taagctgtac tcctttttcc aaaacagtta ctacaacttt aaatatgggt gaagacagca
      361 gaatcgctga taacagcatc ccccctgatt ttacacagtc ggatattccg caagatcccg
      421 acacaaatga atcagttaca accgacaaac catcctccct aattccgcaa atagataaac
      481 cctggtttat agaacgctct tcggtaacaa cagtgcttgg cgcaattatt accgggcttt
      541 tagcgggcct gttccttaat attatgccat gtgttttgcc cgttatttct ttaaagctta
      601 tgtatcttat aaaaaacagc gggaaaagcc gttcaatgat tattactaat tcgatattct
      661 attcattggg aattattttc acatttgtta tactcgcgtc acttgcggca ttcgccgggc
      721 ataattgggg ttcccttttt cagaatactg cgtttatcag ggtaatgata gtaataattt
      781 tcactatggc tctttctcta tttggagttt tcaccttttc aacaccccct attttatcat
      841 ctctttctgg aaaattttcg ggaaacataa cttctgcact ttttaacgga tttcttgcaa
      901 cagttcttgc cacaccatgt agcggcccct ttttaggagc aacactggcg tggacgctta
      961 cacaaccacc gttgattatt tatgccgtct ttctaagcat aggtaccggt ctggctttac
     1021 ccttctttgt attaacgctt tttccaaaac tgatatcgta tattccaaaa agcggatcct
     1081 ggtcacttta cagtgaaaga ctttttgggt ttattcttgc agcaagtgct gtatactttt
     1141 tatcaatact agagagccgg gctcttatcg gaacattgat tcttatctgg tttgtgtcta
     1201 tcggtctttg gcaactggga caattcgcaa caccagtgag ttctaagaaa agcaggacaa
     1261 tttcttatat tattctgttg tttcttgttt tagccggtta caggctctct gtttattaca
     1321 gcaaaccggt taattcttct ataggtacta ttgattacag ccattcacta ttaaatgaac
     1381 tgaataataa cgaaacaatt gttgctgtta aatttactgc cgactggtgt tctaactgta
     1441 aatatgtaga aatgactgtt tttaacaaag atagtgttaa acaggaattt gaaaagaaag
     1501 gtctctcatt attaaaggcc gatattaccg ctcaaggaac ccaagccgaa gagctactat
     1561 cggctcttgg aagccgttcc ataccttttt tggctctttt ttatccggga gaaggtcggc
     1621 aaaatccatt atgtctggat gatatttact ctgaaaatga tttattgaaa ataattgaaa
     1681 ttgctactag acaagccgat acgcaaaaca attcagaaaa agaggaaaaa gcagataagc
     1741 ccgaaaagat tgaagcgcta acagagattg cgccataaaa gttaaacttt attcttgact
     1801 ataaagccga cctaatattt ttagataccg gatcgaaaga cagcacgtta taatgaatcc
     1861 atagcggctt tataccgcct tccaaatgat ccttaccagg gaccgcgtac cgcttaaagg
     1921 tatttctgtt gctgttataa aatcacagac aaaagaggat attatgagct gttcagttgt
     1981 aattggagcg caatggggag acgaaggcaa agccaagatg atagactttt tctcccaaaa
     2041 cgcggatatt attgtccgct accagggggg agccaatgcc ggtcatactg tagtggcaaa
     2101 tggcgaaaaa catgtatttc atcttatacc ttccggaatt cttcatcctg gtaaaatatg
     2161 tgttttagga aacggggttg ttcttgatcc tatgcagcta caaaaagaga tggataacct
     2221 aacggctcaa tcgatagata taaattaccg gcttcttatt tctgatgccg cacatcttat
     2281 acttccgtat cataaggcac ttgatagcgc actcgaagag cttcgcagca acaagatcgg
     2341 aacaaccggt cgagggattg gtccctgtta tgctgacaaa tgtctaagaa ccggaataag
     2401 agtaggcgac atctttcttg acgaaaaaac tcttgaaaag aaggttcggc aaacactaga
     2461 agttaaaaat ctgcaactcg taaagatgca caaaaaagat ccgttttcta ttgaagagat
     2521 aatggatata attctggatt tcaaaaaaca cactcaaaag atgattatta ataccgggta
     2581 ttatcttaac gaagaggcga aaaagaataa aacaataatt cttgaaggtg ctcagggttt
     2641 tggtcttgat attgatcacg gaacatatcc ctttgtgaca tcttctaatc caacaatagg
     2701 tggagccctt ctgggatcgg gtcttaatca ttcggttata gacaaggtta tcggaatctc
     2761 taaagcatac attacacgtg ttggtgaagg accttttcca actgaagatt ttggtgacga
     2821 cggtaacacc ctaagagaca aaggtggcga atacggatca accactgggc gccccagacg
     2881 ctgcggttgg tttgatgtag agctaatgaa acagactgct cgcatctgtg gtctgactga
     2941 cattactctt acaaagatag atgttctttc aggatttgaa aaagttaaag tcgctgttgg
     3001 atacgaatat aagggcaaaa aaatcgatat gtttccatct tatttgcttg atgatatcaa
     3061 gccaatttac gaagagcttg atggatggac tgaagatata tcagaatgta aaagctacga
     3121 agatcttccg caaaacacca gggactatat agactttctg gaaaaaagcg ttggtgttcc
     3181 gatgtctatt atttcggttg gacctgatcg ggaaaatact attgtacgtg atatttggca
     3241 gcaataaaat aatcttaatt ggatccttca taataataaa gctctctttt tccggagggc
     3301 ttttattatt taactcacaa ttttttcatg attaaaatta ttcataaccg ttttttatta
     3361 tttgcactta acattttctg atacagtttt cattaatatc ttcaactttt tgacaaccgg
     3421 tcaaaatcat acatttcata aattcttctt taattgtatt caagtaataa gtaactccat
     3481 cctcttccat cccaacggcg gcaacacaaa tagggcgtcc aatagaaacg gcatcggctc
     3541 caacagctaa ggctttaaac acatcaatac cacttctaaa accgccgtct gcgattattg
     3601 tgattttccc tttcacctct ctgcatattt caggaagcac atccataatg cctggcatct
     3661 gatccaaaac tcttccacca tgattggata ccacaatgcc agctgctcca gtagcaacgg
     3721 catcctttgc gtcttcaaca gacattactc ccttcaagac gaaaggaagt ttactgttgg
     3781 taattatttc ttttaatttg tcaacaccgc gcgctcccgt tgcctgattt cgtaaattca
     3841 tcgtctttag tacaattgcg tcaatatcca ctccgattgc gatggctccg gcatcttcag
     3901 cctctttaat acgtgtcagt aactcctttt tctcttttcg tggtttaaaa ataggtatac
     3961 cacctccaaa ctcctttatc gcgtttaaac caaccctgta tttttcggga gaggctccgt
     4021 caccaaccat tgcaactatt ccacattgcg ccgacccttt tacaacagct ttattatata
     4081 aaaattcatc aattgccccg ttcatatttg tgacggttcc ggtaatcggg gaggcaaaaa
     4141 ctggcatatc cattttcttg ttaaaaagac ctattgacat atccggatta ttaacatcgt
     4201 gaattactct tgttatgatc ttgtactcgg ccaacgaact tatgtttctc ttaaaagatt
     4261 ctccggtgcc cacagcgccc attccgggaa tcgaagaggt acagttctca ccgttacaat
     4321 ctttacagca gatgcaagcc ttgccaaatt tttcacgggc ttttgccctg ataatatcca
     4381 tggatacttc gttgtctgat gctatcttta tcgaaatagt attacgtgcc tttttaacag
     4441 cctcttcagc ctcttccaat gtattcgcgg tggcaatgat atgaccagct ttttcaacat
     4501 tagattttgg tttaacaacc ctgtctcccg gaacaacatt tataaaaata tcatttattc
     4561 ccttgattgc cgtggcttcc tgtaaattat ctatcgattt aattatgcca ggttctgtaa
     4621 ttatcgctct ctcgattgat accttactta tcaccggttc cagattgccc ggctcttgcc
     4681 caagagcaat ctctatcgcc gctttcataa gatctacacc ggtcgaatat ggaaaggtat
     4741 gggtcgacat aaagccgccg gacaaacgtg ccgcaagttc tccaatcttt accccgtcgg
     4801 gagtaacttt aatatctcct ttggcgtaac catgattaat tcccaatgct ttaattccta
     4861 gtttcattac ccgttttgat tcttcgatta tttcagaatc aagctgcgac ggcatcgtat
     4921 gacctttttc aataaaatat ggaaagtctt cgataatacg atcagcaatg cctgtaaagg
     4981 taacttctcc atcaaaaaca acggcatcta tcgaaagttc tggcccttcc atatactctt
     5041 ccataataag ctcaccagag ggtgaagcac tctttgcggc atgaaaggca tctgaaatct
     5101 cgttcttgtt atttacacag cgaactcctc tggc
//
LOCUS       DAOTGZ010000198         5089 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_3064
            NODE_19458_length_5089_cov_4.913786, whole genome shotgun sequence.
ACCESSION   DAOTGZ010000198 DAOTGZ010000000
VERSION     DAOTGZ010000198.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868088
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 5089)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5089)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:22:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,785
            CDSs (total)                      :: 2,751
            Genes (coding)                    :: 2,732
            CDSs (with protein)               :: 2,732
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 12 of 19
            Pseudo Genes (internal stop)      :: 5 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5089
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_19458_length_5089_cov_4.913786"
                     /isolate="AMR_MDS_3064"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..238
                     /gene="fliN"
                     /locus_tag="P5123_11170"
     CDS             <1..238
                     /gene="fliN"
                     /locus_tag="P5123_11170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013968984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009288 - bacterial-type flagellum
                     [Evidence IEA];
                     GO_function: GO:0003774 - cytoskeletal motor activity
                     [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="flagellar motor switch protein FliN"
                     /protein_id="HRX16865.1"
                     /translation="LTLTAELGRTKYLVKDVLGLGEGSIIELDKLAGEPVDLLVNGKL
                     IAKGEVVVIDENFGVRVTDIVSPSERLTKMGSGS"
     gene            447..1244
                     /locus_tag="P5123_11175"
     CDS             447..1244
                     /locus_tag="P5123_11175"
                     /inference="COORDINATES: protein motif:HMM:NF016250.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthetic protein FliO"
                     /protein_id="HRX16866.1"
                     /translation="MRKRLIDKSSRFRSFLCFAIIVFVCNSIYLQSVYAQQTEEQNEK
                     KVVAESSENNSESMSQALDESLYQYEQPESEDVSYGWMIFRALFIIALLVLGFYLFIR
                     YLTRQGAVQVAGAGVASTISIMPLGQNRQLHIIELGTKVLVLGVCDGGVNLIKEVSDR
                     DEIDRIKLQSSRSSAMPGTGFQQFLKNQIEQIADKVSKGTSARRVISKPSPIKAPVKD
                     NFEEMVASSVADEKQSGERSFFDDDRIDYMRKQRTRLREINRYNDED"
     gene            1261..2001
                     /gene="fliP"
                     /locus_tag="P5123_11180"
     CDS             1261..2001
                     /gene="fliP"
                     /locus_tag="P5123_11180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012063832.1"
                     /note="The bacterial flagellar biogenesis protein FliP
                     forms a type III secretion system (T3SS)-type pore
                     required for flagellar assembly; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar type III secretion system pore protein
                     FliP"
                     /protein_id="HRX16867.1"
                     /translation="MTVAFLLMPMASMAQDVGGVQMPIPKIAFDITEAEKPQDVALAL
                     QVLFLLTILTLAPSIALMMTAFTRIVIVLDFVKRALALQQMPPTQVIVGLSIFLTFFV
                     MAPTFKTINENALQPYMKAEISNEVFFERAMTPMRQFMFKQTRKKDIALFMELGEVEK
                     PQSEEDVPSYCLIPAFMISEMRRAFEIGVRIFIPFIVIDMIVASALMAMGMIMLPPVM
                     ISLPFKIILFVLVDGWNLIIYELVKSFL"
     gene            2014..2286
                     /gene="fliQ"
                     /locus_tag="P5123_11185"
     CDS             2014..2286
                     /gene="fliQ"
                     /locus_tag="P5123_11185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012995275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009288 - bacterial-type flagellum
                     [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthesis protein FliQ"
                     /protein_id="HRX16868.1"
                     /translation="MNDVTVIILLREALMITLLVSSPVLVSGMVVGLIVSVFQTTTSI
                     QEQTLTFVPKMIAIFLAIVLSAAWMIHMLVNYTQRLLMQISEIGAI"
     gene            2286..3065
                     /gene="fliR"
                     /locus_tag="P5123_11190"
     CDS             2286..3065
                     /gene="fliR"
                     /locus_tag="P5123_11190"
                     /inference="COORDINATES: protein motif:HMM:TIGR01400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006605 - protein targeting [Evidence IEA];
                     GO_process: GO:0044780 - bacterial-type flagellum assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthetic protein FliR"
                     /protein_id="HRX16869.1"
                     /translation="MLYFIYNFQAFLLVMIRIHSLFVVAPFFSSGVTPVRLKALLAFF
                     ITLVIFPLIVKNGISVSGNMLEYGLMVVREIIIGVYIGFIASIVFTAFQLAGQYFSVQ
                     IGFGFSEVVDPMAQISVPIIGQIKSMVGLLVFLYINGHHFLISAIYRSFELAPVINFS
                     DTAISDHMRYLTYSFSGMFIVALKIALPILATVFLVSVSMGVLAKAAPQMNIMMLGFP
                     FKIMVAFVLIVVLSPLIIRIMQVALGRSFHFISKVLTYWPV"
     gene            3067..4227
                     /gene="flhB"
                     /locus_tag="P5123_11195"
     CDS             3067..4227
                     /gene="flhB"
                     /locus_tag="P5123_11195"
                     /inference="COORDINATES: protein motif:HMM:TIGR00328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthesis protein FlhB"
                     /protein_id="HRX16870.1"
                     /translation="MAEFIQKKYAILSNDDLKKYSLDLQRFADPEDEGRTEEPTEKKI
                     RDARQKGQVAKTEELPQALVVILGFSFIFFLGEWIFNELAMMTKFYITNFNSFYITDR
                     TTLNEGFRVILFLTKILSPIFGICVVAGVVGNVVQIGFEFSAHPIKVDFSKIKLSPAE
                     MIKKILISKRVAMNLFKTLFKIAVVGFSSYLIISADFGVVLNSPDISVAAALSKVCFI
                     AYKIIISSAILLMVLALPDYIFQKQEFMESLKMSKQEIKEEFKESQGDPHVRARLREM
                     QRDILTRNMIREVPKADVIVTNPTHFSIALKWDQHVMQAPSVIAKGVDSMALRIKEVA
                     RANNIVMIENRPLAQALYKDVEIGQEIPAELFKAVSEVYSILYERGRLGSVV"
     gene            4288..>5089
                     /locus_tag="P5123_11200"
     CDS             4288..>5089
                     /locus_tag="P5123_11200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013968978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009306 - protein secretion [Evidence IEA];
                     GO_process: GO:0044780 - bacterial-type flagellum assembly
                     [Evidence IEA];
                     GO_process: GO:0071978 - bacterial-type
                     flagellum-dependent swarming motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthesis protein FlhA"
                     /protein_id="HRX16871.1"
                     /translation="MAEASMTSVLRNGDWMHRKDLLLGIGVISVVAMLIIPLPTFLLD
                     FLMIISIAIGLLTLLIVMFVPKAYDFSIFPTLLLVTTVFRLALNVSSTRLILLQGAAF
                     DGKIVKTFGSFVVGGNYVIGFIIFIILVAVQFIVITKGATRTAEVAARFTLDSLPGKQ
                     MTIDSDFANGVITEEEANRRRSELRKETDFYGAMDGASKFVQGDVKVGILITFINILG
                     GFIIGMVMRGESFEVASQTYSLLTIGDGLVGQIPALIISTAAGIIVTRS"
BASE COUNT         1397 a          827 c         1122 g         1743 t
ORIGIN      
        1 ccttacatta actgctgaac tggggcgcac aaagtattta gttaaagatg ttcttgggtt
       61 gggggaaggc tctattatcg agcttgataa gctggctggt gaacctgtag acttgttggt
      121 aaatggaaaa ttgattgcta aaggcgaagt cgtggttatt gacgaaaact ttggtgttcg
      181 tgtaacggat attgtttcac cttcggaacg tttaacaaaa atgggtagcg gatcctgatt
      241 cgctactggt tattgtaata taaaaattcc taataaacgc taataaataa atttggcgtt
      301 taacattttc ttataagaag agggtgtctg tttagggaat gtttcttggc gttggtgcgt
      361 tatttaatga gacataagat ttatatctga tgaagatatt cgttttattg gcagaaaata
      421 gttgtgatta gggtaaataa aaagatatga gaaaaagatt aattgataag tcatcaagat
      481 ttcgcagttt tctttgtttt gcgataattg tttttgtatg caattccata tatcttcaat
      541 cggtatatgc tcagcaaact gaagaacaaa atgaaaaaaa agttgtcgca gagtcatcgg
      601 aaaataacag cgaatcgatg tcgcaggctt tagacgagtc tctttatcag tacgagcaac
      661 cggaatccga agatgtctct tatgggtgga tgatttttcg ggcattgttt attattgctc
      721 ttcttgttct tggattttat ctttttattc gttatttaac tcggcaggga gctgtgcaag
      781 tggcgggagc cggggttgct tcaacgattt ctatcatgcc tctcggacaa aatagacagc
      841 ttcatattat cgaacttgga actaaagtat tggttctggg cgtgtgcgat ggtggagtaa
      901 acttgatcaa agaagtttcc gatcgtgatg agatagaccg aatcaaactt caaagttcgc
      961 gctcctctgc gatgccggga acaggttttc aacaattttt gaaaaatcaa attgaacaga
     1021 ttgctgataa ggtttccaaa gggacttctg ctcgccgtgt tatcagtaaa ccgtctccga
     1081 ttaaggctcc tgttaaagat aattttgaag agatggttgc ctcttctgtt gcggatgaaa
     1141 aacagtctgg cgaacgttcc ttttttgatg atgaccgcat cgattacatg cgcaaacaac
     1201 gcacgcgact gcgtgagatt aacaggtaca atgatgaaga ctaggttatt acttctactt
     1261 atgactgtag cgtttctttt gatgccaatg gcatctatgg ctcaggatgt gggtggagta
     1321 cagatgccca tacctaaaat agcgtttgat atcacagagg ccgaaaaacc ccaggatgtg
     1381 gcacttgcgt tgcaggttct cttcttgctg actatattaa cgctggctcc gtcaattgca
     1441 cttatgatga cagcttttac gcgaattgta atagttttag attttgtaaa acgggctttg
     1501 gctctccagc agatgccacc cacacaggta atagtcggtt tatccatatt tttaaccttt
     1561 tttgtaatgg cgccaacatt caaaactatt aatgaaaatg ctctacagcc ctatatgaag
     1621 gctgaaattt caaatgaggt tttttttgaa agagctatga cgccgatgag acagtttatg
     1681 tttaagcaga ctcgtaaaaa ggatattgca ctttttatgg agcttggcga agtagaaaaa
     1741 cctcagtctg aagaggatgt tccttcttat tgccttatcc ctgcgtttat gataagcgaa
     1801 atgagaagag cctttgaaat tggtgtcagg atatttatac cgtttattgt tatcgatatg
     1861 atagtcgcga gcgcgcttat ggcaatgggt atgataatgt tgccgcctgt aatgatctct
     1921 cttccattca aaataatact gtttgttctt gtggatggat ggaatcttat aatctacgaa
     1981 cttgttaagt catttttgta aaggagtccg attgtgaatg atgtaacggt cattatcctt
     2041 ttaagggaag cactaatgat tactctgctt gtctcatccc ctgtgcttgt ctcaggtatg
     2101 gttgtcggac ttatcgtctc tgtatttcag acaactactt ctattcaaga gcagactctt
     2161 acatttgttc ctaaaatgat tgcgatattt ttagctatag ttctatctgc ggcatggatg
     2221 atacacatgt tggttaatta tactcaacga cttttaatgc aaatttcaga aataggtgcc
     2281 atatagtgct ctattttata tataattttc aggccttttt actggtaatg attcgtattc
     2341 attcactgtt tgttgtagct ccgttttttt ccagcggagt aacgcctgta aggctcaagg
     2401 ctctattggc tttttttatt actctggtta ttttcccgct catagttaaa aacgggatta
     2461 gtgtctctgg aaatatgctt gagtacggtc tgatggttgt tcgcgaaata attattggcg
     2521 tttatattgg gtttatcgct tcgattgttt ttacggcttt tcaattggcg gggcagtatt
     2581 tttcggtgca gataggtttt ggtttcagtg aagttgttga tccgatggct cagatctctg
     2641 tcccgattat tggtcagatt aagagcatgg tggggttact tgtgtttctg tatattaacg
     2701 gacatcattt tttaatcagt gctatatatc gttcttttga gttggcacct gtgattaatt
     2761 ttagtgatac cgccatatct gatcatatgc gttatttgac atattcgttt tctggaatgt
     2821 ttattgttgc tctgaaaata gcattaccca ttttggcaac tgtatttctt gtttctgtat
     2881 caatgggggt tttggctaag gctgcaccac agatgaatat aatgatgctt ggttttccat
     2941 ttaagataat ggtggcattt gtattaatag tggttttgtc tcctctgatt atcagaataa
     3001 tgcaggtcgc tttggggcgt agttttcatt ttatatctaa agttttgaca tattggccgg
     3061 tttaaaatgg cagaatttat tcagaaaaaa tatgccattt tatcaaatga tgatcttaag
     3121 aaatatagtc ttgatcttca gaggttcgcg gatccggaag atgaaggtcg taccgaagaa
     3181 ccgaccgaaa agaaaattcg cgatgcgcgc caaaagggac aggttgcaaa aacagaagaa
     3241 cttccacagg cccttgtggt tatacttggt ttttctttta tcttttttct tggagaatgg
     3301 atttttaatg aactggctat gatgaccaag ttttatataa caaatttcaa tagtttttat
     3361 attacagatc gtacaacatt aaacgaaggt ttcagggtaa tacttttttt aacaaaaata
     3421 ttgtcgccaa tatttggaat atgtgtagta gccggagttg ttggcaatgt tgtacagatt
     3481 ggttttgagt tttcggctca tcctattaag gtcgatttca gtaaaattaa attaagtccc
     3541 gcggagatga ttaaaaagat attaatatcg aaacgtgtag cgatgaatct gtttaagact
     3601 ctttttaaaa ttgctgtcgt tggtttttcg tcgtatctga taatttcagc tgattttgga
     3661 gtagtgttaa attcgcccga tatatctgtc gcggctgctc tttcaaaggt ctgttttata
     3721 gcttacaaaa taattatatc atcggctatt ttgctaatgg ttcttgcctt gcccgattat
     3781 atatttcaaa agcaggaatt catggaatct ttgaaaatgt cgaagcagga gatcaaggaa
     3841 gagtttaagg agtcacaggg agatccccat gttagagcaa ggcttcgaga aatgcagcgt
     3901 gatatattaa caagaaacat gattcgagag gtgcccaagg cagatgtgat cgttacaaac
     3961 ccgacacatt tttccatagc attaaaatgg gatcagcatg tcatgcaagc gccatctgtt
     4021 attgcaaaag gtgtcgattc aatggctctt cgtataaaag aggtcgctcg agcaaacaat
     4081 attgttatga ttgaaaatcg gccattagca caagcactct ataaagatgt agaaattggt
     4141 caagagattc ctgctgaact gtttaaggct gtttcggaag tttatagtat actgtacgag
     4201 agaggacggc ttggatcggt tgtctgataa ttaaaatatt ggtaatagtt taacaattct
     4261 aaagataatt attaaaggga gagaacaatg gcagaagcat caatgaccag cgtattgcga
     4321 aacggtgact ggatgcatcg taaagacctg ttattgggta tcggtgttat ttctgttgtt
     4381 gcgatgttga taattccatt acctacgttc cttcttgatt ttctaatgat tatcagtatt
     4441 gctattgggc ttttaacttt attgattgtt atgtttgttc caaaagctta cgacttctcc
     4501 atttttccga cacttctgct cgttacaacg gtatttcgat tggcacttaa cgtctcttca
     4561 actcgattaa ttcttttgca gggtgcagca tttgatggaa aaatcgtcaa aacttttgga
     4621 tcctttgttg ttggaggaaa ctatgtaatc ggttttatta tctttatcat tcttgttgca
     4681 gttcagttca ttgtaattac caaaggtgcg acacgaacag cagaagtagc agcacgtttt
     4741 actttggatt ctttgccagg taaacagatg accatcgatt cagattttgc caatggtgta
     4801 atcactgaag aggaggctaa ccgaagaaga agtgaacttc gaaaggaaac cgacttttat
     4861 ggagcaatgg atggagctag taagtttgtt cagggagatg ttaaggttgg tatacttatt
     4921 acctttataa atattttagg tggttttatt attggaatgg ttatgcgtgg ggaaagtttc
     4981 gaggttgcaa gtcaaaccta ttccctgtta accattggtg atggtttagt aggtcagatt
     5041 cctgctttga ttatttctac cgctgccggt atcattgtta cccgttcag
//
LOCUS       DAOTGZ010000199         5027 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_3064
            NODE_19783_length_5027_cov_4.836082, whole genome shotgun sequence.
ACCESSION   DAOTGZ010000199 DAOTGZ010000000
VERSION     DAOTGZ010000199.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868088
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 5027)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5027)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:22:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,785
            CDSs (total)                      :: 2,751
            Genes (coding)                    :: 2,732
            CDSs (with protein)               :: 2,732
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 12 of 19
            Pseudo Genes (internal stop)      :: 5 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5027
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_19783_length_5027_cov_4.836082"
                     /isolate="AMR_MDS_3064"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..897)
                     /locus_tag="P5123_11205"
     CDS             complement(<1..897)
                     /locus_tag="P5123_11205"
                     /inference="COORDINATES: protein motif:HMM:TIGR00341.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00341 family protein"
                     /protein_id="HRX16872.1"
                     /translation="MDENKKTNQRKNPKDADTEDNTADIESKIGAEESEDESLIASFS
                     KKFSLGKKALQERAESLNNDFQQAVEQTDETIKRINKHSTLLSMITRPADWKSTYSEY
                     KARRNKEKEDLNVYGILFDGASPSIEYYMLTILSCVIATTGLIQGSTAVIIGAMIVAP
                     LMTPILASSLGVIWGDFKLMRMSFFSLVKGAILAILISSALAYIIPLSSYSDEILART
                     TPSLFDILVALASGSVGAYGYANKKINNTLVGIAIAVALMPPLCTIGIGLGTLNSQIA
                     IGATVLFLINYVSISLAGAIIFM"
     gene            complement(935..1888)
                     /locus_tag="P5123_11210"
     CDS             complement(935..1888)
                     /locus_tag="P5123_11210"
                     /inference="COORDINATES: protein motif:HMM:NF024109.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HRX16873.1"
                     /translation="MTITYKPSLLFKNPHINTIYASKFRKVDEVYTSKFRLELSDGDF
                     LNIDTRITGSKRAIVIVHGLGGHSKRTYVMGLAKAFINLGYDIFGVNLRGCGGETNRL
                     IKTYHAGKYDDVDEIVKHISVKYKYQDIGIAGFSLGGVITSRYFIESGSSSLASFGIA
                     ISAPFDLATTSELLSNSRFYMSYFLKKLKREMQKKADIFDEISMEDYDLIKTFAQYDD
                     RYTAPLNGFKSAVDYWKKSSTASRLKTIKKPFLIISSEDDPVLSRESLPYEIDKINSY
                     INVQITKYGGHTGFLEKTGVPCWHEKCSALFVKKIGLTTNN"
     gene            complement(1919..2443)
                     /locus_tag="P5123_11215"
     CDS             complement(1919..2443)
                     /locus_tag="P5123_11215"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRX16874.1"
                     /translation="MGFKIKDTILKATGYIFAIPGLIVFFIGIAMYLVSKDPTEIEAG
                     AGIAIASMAFVLPGIFFLYQGYKATKEEEMVESLAMIIHATRRISIDNIAEKMGVSVP
                     TARRFMLMALKLRLINGFFDRTTGEFFTEDGKTDTSPFKFCPHCGSPFNKIFLHGETI
                     KCPACGSLFSGNNQ"
     gene            complement(2524..3495)
                     /locus_tag="P5123_11220"
     CDS             complement(2524..3495)
                     /locus_tag="P5123_11220"
                     /inference="COORDINATES: protein motif:HMM:NF020476.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1848 domain-containing protein"
                     /protein_id="HRX16875.1"
                     /translation="MKKWPLGFIGDDPQKKPARSPLIISASRATDIPAFYGDWLVNQF
                     DQGFVNWINPYNRKQNPIDLRNCRLIIFWTKNAAPFLKYLSYFDSKGINYYFLHTLNN
                     YETEGYEPGVPPLKKRIRTFKMLSDLIGPEKIIWRFDPILLSDKLTLKELLLRIRQLS
                     KELSPYTRRLIISFIKIERYKSVKRNIIKTSFPVRELNHNEKIFTAESIQHIFSNSSE
                     KPVTVQTCGTAEDFSSFGINHGSCIDYNLITDLFHNDTRLMDFLEYTPSLFDNDNTMP
                     SALKASGQQPFCNCLQSADIGAYNSCPHHCVYCYANHTPDIIKKSLD"
     gene            complement(3523..3840)
                     /locus_tag="P5123_11225"
     CDS             complement(3523..3840)
                     /locus_tag="P5123_11225"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="inorganic pyrophosphatase Ppa"
                     /protein_id="HRX16876.1"
                     /translation="MEQHQYIQFLLENKGSNSIQRVDTRHDFIEFVGTPKKHPVDKNI
                     LVLLTNPFEKNKHYYEFSLDSICDIEELNSVSADNGETAYQVRIWVHKGSLAFKTEPF
                     IVT"
     gene            complement(3947..>5027)
                     /gene="glnS"
                     /locus_tag="P5123_11230"
     CDS             complement(3947..>5027)
                     /gene="glnS"
                     /locus_tag="P5123_11230"
                     /EC_number="6.1.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015852886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004819 - glutamine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006425 - glutaminyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="glutamine--tRNA ligase"
                     /protein_id="HRX16877.1"
                     /translation="RTGDKWCIYPMYDFTHCLSDAIEGVTHSVCTLEFKNNRDLYDWV
                     LQAVNFEHRPRQIEFARLNLNYTVMSKRLLLQLVKEKHVSGWDDPRMPTISGLRRRGF
                     TPESVRDFTSRIGVAKADNIVDIALLEHCLREDLNKKADRAIAVLNPLKLIIENFPEN
                     EELMLEAKNNPEDDSAGSRMLPFTKELFIERDDFKTEAPKKYFRLAPGMEVRLKFAFY
                     VTCTGFSTDDKGNITEVRCTYDPDTKGGWSQDGRKVKGTIHWLSAKHARNAEVRIYDR
                     LFQTANPYDASEGKTFLDNLNPESLTTLKNVKIEESLANAEPGYICQFERTGYFCSDI
                     KDHTHERPVYNRAVPLRDSWAKIDN"
BASE COUNT         1468 a          907 c          993 g         1659 t
ORIGIN      
        1 cataaagatg attgcacctg ctagtgatat tgaaacatag tttattaaaa aaagaactgt
       61 tgctccgata gctatctgac tgttcaatgt gccgagacct attccaatag tgcacagtgg
      121 cggcataaga gctacagcta ttgctattcc gacaagtgtg ttgtttattt ttttatttgc
      181 atagccgtag gctccaacag aaccggatgc aagagcaaca agaatatcaa aaagtgaggg
      241 agttgtgcgt gctagtattt catccgaata tgatgaaagc ggtattatgt aagcaagtgc
      301 tgatgatatc aggattgcaa gaatggctcc cttaacgagt gagaagaaag acatccgcat
      361 cagcttgaaa tctccccaaa taactcctag agaacttgcg agaattggag tcataagtgg
      421 ggcgactatc attgctccta ttataaccgc ggtagagccc tgtatgagac ccgtagttgc
      481 aataacgcag gaaagaatgg ttagcatgta atattcaatt gaaggtgaag caccatcaaa
      541 aaggattccg tatacgttaa ggtcttcttt ttctttgtta cgtcgggctt tatattcact
      601 gtaagtactc ttccagtctg ccggtcgggt aatcatgctt agaagagtag agtgtttatt
      661 gatgcgttta atcgtctcat ccgtttgttc tactgcttgt tgaaaatcgt tgttgagtga
      721 ctcggctctt tcttgaagcg cttttttgcc aaggctgaat ttttttgaaa atgaggcaat
      781 aagactctcg tcttctgatt cttcagcgcc aatttttgat tcaatatcgg cagtgttatc
      841 ttcagtatct gcatctttag ggtttttacg ctggtttgtt tttttatttt cgtccatact
      901 gatcacctca aagtgaattt tcagaatagt aaaactagtt atttgttgtt agcccaattt
      961 tttttacaaa aagagcagaa catttttcat gccaacatgg tactccagtt ttttcaagaa
     1021 aacctgtatg accgccatat tttgttattt gaacgttaat atagctgttt attttatcga
     1081 tttcatatgg aagactctct ctacttaaaa ctggatcatc ttcagaggag ataattagaa
     1141 agggcttttt tattgtttta agtcgtgatg ctgtacttga ttttttccag taatcaaccg
     1201 cagatttaaa tccgttaaga ggagcggtgt accggtcatc gtattgagca aaggttttta
     1261 ttaaatcgta atcttccatg gaaatttcat caaaaatatc tgcttttttt tgcatttcac
     1321 gtttgagttt ttttagaaaa tagctcatat aaaaacgaga atttgagaga agttcagatg
     1381 tcgttgctag atcaaatgga gctgaaattg caatgccaaa agaagcgagt gaagaactgc
     1441 cgctttcaat aaagtatctg gaagttatca ctcctcccaa agaaaaacca gctattccaa
     1501 tatcttgata tttgtacttg acactaatat gctttacgat ttcgtcaaca tcatcatatt
     1561 tgccggcatg atatgtttta atcaggcggt tggtttcacc tccgcaacct ctcaagttta
     1621 ctccaaagat atcatagcca agattaatga atgctttggc cagacccata acgtaggttc
     1681 ttttagaatg tcctcctaat ccatgtacaa ttacaattgc tcgcttggaa ccagtaattc
     1741 gagtgtcgat atttagaaaa tcaccgtctg atagctctag acgaaattta gatgtatata
     1801 cctcatcgac tttacggaac ttggaggcgt aaatagtatt gatatgagga tttttaaata
     1861 gtagactagg tttatatgta attgtcatga gaaagtcctt attaaaagaa ttaagaaatt
     1921 actgattatt acctgaaaaa agagaaccgc atgcaggaca cttgatcgtc tcaccgtgta
     1981 aaaatatttt gttaaatgga gaaccacaat gcggacaaaa cttgaacggc gaagtgtctg
     2041 ttttcccgtc ttctgtgaaa aattcacctg ttgttcggtc aaaaaagccg ttaattaatc
     2101 ttagtttgag agccatcaac atgaatcggc gtgcggttgg aaccgaaacc cccatctttt
     2161 ctgcaatgtt gtctatggat atgcgccgtg tcgcatgaat aatcattgca agcgattcga
     2221 ccatctcctc ttctttcgtt gccttgtatc cttggtaaag gaaaaaaatc ccgggcaaaa
     2281 caaaagccat tgaggctatt gctattccgg caccagcttc aatttctgtc ggatctttgg
     2341 aaactaagta catagcaata cctataaaaa acacaatcag accgggaata gcaaatatgt
     2401 aacctgtagc ttttagaatg gtatctttta ttttgaatcc catgagtgac tccatagtat
     2461 aattgactta acacaattta cactaaatta tagatgagta gcaactaaaa aaccaatttt
     2521 aaatcaatcg agtgattttt ttattatatc aggagtgtga tttgcataac agtatacgca
     2581 atggtgtgga cagctgttat atgctccgat atcggcactt tgaagacagt tgcaaaaggg
     2641 ctgttgtccg gacgccttta aggcagatgg cattgtatta tcattatcaa atagtgatgg
     2701 agtatactct agaaaatcca taagccgggt gtcattatga aatagatctg ttatcaggtt
     2761 gtagtctata cagcttccat gattaattcc aaacgaggag aagtcttcag ctgttccgca
     2821 tgtttgaact gttacaggtt tttctgaaga gttactgaat atatgttgta ttgattctgc
     2881 agtgaaaatt ttctcattgt gattaagttc tcttactggg aaagaagttt ttataatatt
     2941 gcgctttacc gatttatatc gttcaatttt aataaaactt ataattagcc ttcttgtgta
     3001 gggggaaagt tcttttgaca attgcctgat tcttaacaaa agttctttta atgtgagttt
     3061 gtctgataaa agaatagggt caaaacgcca tatgattttt tcagggccaa ttaagtcgga
     3121 taacatcttg aaggtcctaa ttcttttttt tagcggaggt acaccaggct cgtaaccttc
     3181 tgtttcgtaa ttattcaagg tgtgtaaaaa atagtagttt attccttttg aatcaaaata
     3241 cgacagatat ttgagaaaag gtgccgcatt ttttgtccag aaaatgatta ggcgacagtt
     3301 tcgcagatca attgggtttt gttttctgtt atatgggttt atccagttta cgaatccttg
     3361 atcaaattgg ttaacaagcc aatctccata aaaagccgga atatctgtgg ctcgacttgc
     3421 agagataata agaggggaac gagccggttt tttttgagga tcgtccccta taaaaccaag
     3481 aggccatttc ttcataattt tctataatca gaattatgca aatcaggtca caataaatgg
     3541 ttcggtttta aatgccagtg agcccttgtg aacccaaata cgtacctgat aagctgtttc
     3601 tccattgtca gcagaaactg agtttaattc ttcaatatca catatgctat cgagagaaaa
     3661 ctcataatag tgcttgtttt tttcgaaggg atttgtaagt aaaaccagaa tgtttttatc
     3721 aactggatgt tttttgggag tacccacaaa ttcaataaaa tcatgtctgg tatcgactct
     3781 ttgaatggag ttagaaccct tgttttcaag aagaaattga atgtattgat gctgctccat
     3841 aactagactc ctgaaatata ttaatcaaat ttattcgtat cctttttatg ataataaaaa
     3901 tgaacttatg tttcaataca actttctgtt gcttaatatt taaaacttaa ttatcaattt
     3961 ttgcccacga gtcccgtaga ggtacagcac gattgtagac aggtctttcg tgtgtgtgat
     4021 cctttatatc gctgcaaaaa taaccggttc tttcaaattg acaaatatat ccaggttcag
     4081 cgtttgccaa cgactcttca atctttacgt tttttagagt ggtgagtgat tccgggttca
     4141 gattatccag aaatgtttta ccttccgagg catcgtaagg gtttgcagtt tggaaaagcc
     4201 tatcgtatat acgtacttca gcatttcttg catgtttggc tgatagccag tgaattgtgc
     4261 cctttacttt tcgtccatcc tgtgaccagc cgccttttgt atcgggatca tatgtgcaac
     4321 gaacttctgt tatgttgcct ttatcatcgg tactaaaacc ggtacaggtt acataaaagg
     4381 cgaatttaag acgaacttcc atacctggag ctaacctgaa atactttttg ggggcttcag
     4441 ttttgaagtc atcgcgttcg ataaataatt cttttgtaaa tggaagcatt cggctgcccg
     4501 cagaatcatc ctctgggtta ttttttgcct cgagcattag ctcttcattt tctggaaaat
     4561 tttcgataat aagttttagc ggatttaaaa cagcaatggc tctatccgct tttttgttaa
     4621 gatcttctcg aagacagtgt tctaaaaggg cgatgtccac gatattatcc gctttagcga
     4681 caccaatacg tgatgtaaag tcgcgaactg actcgggagt aaatccccgg cgtctgagac
     4741 cggaaatggt gggcatgcgt ggatcatccc atcccgatac gtgtttttct ttgacaagtt
     4801 gaagaagtag ccttttgctc atgactgtat aattaaggtt aagtcgcgca aattctatct
     4861 gtcttgggcg atgttcgaaa ttgactgcct gtaaaaccca gtcataaaga tctcgattat
     4921 ttttaaattc aagtgtgcaa accgagtggg taaccccttc aattgcgtca gaaagacagt
     4981 gtgtaaagtc gtacataggg tagatgcacc atttatcgcc tgtgcga
//
LOCUS       DAOTGZ010000200         5025 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_3064
            NODE_19801_length_5025_cov_5.359356, whole genome shotgun sequence.
ACCESSION   DAOTGZ010000200 DAOTGZ010000000
VERSION     DAOTGZ010000200.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868088
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 5025)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5025)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:22:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,785
            CDSs (total)                      :: 2,751
            Genes (coding)                    :: 2,732
            CDSs (with protein)               :: 2,732
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 12 of 19
            Pseudo Genes (internal stop)      :: 5 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5025
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_19801_length_5025_cov_5.359356"
                     /isolate="AMR_MDS_3064"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            9..1115
                     /gene="ychF"
                     /locus_tag="P5123_11235"
     CDS             9..1115
                     /gene="ychF"
                     /locus_tag="P5123_11235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002779614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="redox-regulated ATPase YchF"
                     /protein_id="HRX16878.1"
                     /translation="MALSIGIVGLPNVGKSTLFNSLSKSAHAEAANYPFCTIEPNSAV
                     VPVPDRRIDALCDIVKPQRALNTTVEFTDIAGLVKGASKGEGLGNQFLSHIRETSAIV
                     HVVRCFEDDNVVHVEGSVDPVRDCEVISTELLLADIQTVEKRIERLEKQSRQDKKLQP
                     MLEFTKQILNHLNAGNPAISFDGRNEDIYEQFSKEIKLITDKKVIFAANVDEDSLANG
                     NSYVDALKEYAIKTGNDHVIMVCAKIEEELISMEEDERIEYLESLGVSAESGLDKIIR
                     VGYDTLGLISYFTAGVKEVRAWTIENGWKAPRAASVIHNDFERGFIRAEVISYDDFIE
                     YKGEAGAKAAGKMRTEGKEYRVADGDIMHFLFNI"
     gene            1521..1718
                     /locus_tag="P5123_11240"
     CDS             1521..1718
                     /locus_tag="P5123_11240"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRX16879.1"
                     /translation="MEAQPEDLHEAVLLYTHTKIELAKRIKEGISYTEFLDYEKKLEQ
                     IDRILKKAERQLEKEVFTGSS"
     gene            2175..>5025
                     /gene="nifJ"
                     /locus_tag="P5123_11245"
     CDS             2175..>5025
                     /gene="nifJ"
                     /locus_tag="P5123_11245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012863031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0016903 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors [Evidence IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA];
                     GO_process: GO:0022900 - electron transport chain
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate:ferredoxin (flavodoxin) oxidoreductase"
                     /protein_id="HRX16880.1"
                     /translation="MGKSMTMDGNMAAAHVSYAFTEVAAIYPITPSSNMAEYTDEWAA
                     HGKKNIFGEPVRLIEMQSEGGAAGAVHGSLQAGALTTTYTASQGLLLMIPNMFKISGE
                     LLPGVFHVSARSVAAHALSIFGDHSDIYSARQTGFAMLATGSVQEVMDLGAVAHLAAI
                     KTRVPFVHFFDGFRTSHEIQKIDTWDYADLEKLVDKEALQKFRDNALNPNNPVTRGTA
                     QNPDIFFQAREACNKFYDAVPDVVADYMKQISKLTGREYKPFMYYGDPKAENIIIAMG
                     SVSDTIKETIDYLNKKGEKVGAIIVHLYKPFSVKHLMEVLPKSVKKIAVLDRTKEPGA
                     EGEPLYLDVKSIFYGKKDAPVIVGGRYGLSSKDTTPSQIIAVYENLKQKESKDGFTIG
                     IVDDVTFKSLPLTAEVNVVPESTKQCMFYGLGSDGTVGANKNSIKIIGDHTDMYAQGY
                     FAYDSKKSGGITISHLRFGKDKIRSPYLVNHADFVACSQPSYLDKYDMLKGIKKGGAF
                     LLNSIWSAEETKANLPDKVKKILAENEVNFYIINATKIAEEIGLGGRTNTILQSAFFK
                     LAEVIPYDDAVKYIKEAIKKSYGKKGDDIVNMNNAAVDKGASELVKVEVPAEWASIKI
                     AADATSDRPDFIKNIADVINGQDGDSLPVSAFIGAEDGTFPAGTTAYEKRGIAVNVPE
                     WQSENCIQCNQCAFVCPHAVIRPLLIDKDEKAKLPSDITTIKAVGKGVEDYEYKLQIS
                     PLDCTGCGNCADVCPAPKGKALVMKPIASQAKEAELWDVVTKNVSYKTDKFPLTNVKG
                     SQFAQPLFEFHGACAGCGETPYIKLITQLYGDRMMIANATGCSSIYGGSAPATPYCKN
                     ANGCGPAWANSLFEDNAEYGLGMYLGVCNMRDRIERLMKEALASGVSGDVEAAFKAWI
                     ENKENGETTREVSTKVEEVLAGKSDAYAKEIMAIKQ"
BASE COUNT         1585 a          795 c         1070 g         1575 t
ORIGIN      
        1 tatggaaaat ggctttatct ataggtatag ttggacttcc taatgtaggg aagtccactt
       61 tatttaacag tctttcaaaa tcggcacatg cggaagccgc gaattatcct ttttgtacca
      121 ttgaaccaaa ttccgcggtt gttcctgtac cagatcgtcg gatagacgcc ctttgtgata
      181 tagttaaacc gcaacgggca ttaaacacga ctgttgaatt tacagatata gctggattgg
      241 taaaaggggc atctaaaggt gaaggacttg gaaaccagtt tttatcacat atcagggaaa
      301 cctcagctat agtacatgtt gtgaggtgct ttgaggatga caatgttgta catgtagaag
      361 gatcggtgga tcctgtgcgt gattgcgaag tgattagtac agaacttctt ttggcagata
      421 tacagactgt tgaaaaaaga atagagcgtc ttgaaaaaca gtcaaggcag gataagaaac
      481 ttcaacctat gcttgaattt accaaacaaa tcttgaatca cctgaacgcc ggcaatcctg
      541 caatatcatt tgatggacga aatgaagata tttatgagca gttctcaaaa gagattaaac
      601 tgattacaga taagaaggtg atttttgcag caaacgttga tgaagattca ttagcaaacg
      661 gtaactcata tgtagatgcg ctaaaagagt acgctataaa aactggaaac gatcatgtta
      721 ttatggtttg cgcaaagata gaggaagagc ttataagcat ggaagaggat gaaaggatcg
      781 aatatcttga atcgttgggt gtttctgctg aaagtggctt ggataagata attagagtgg
      841 gttatgatac ccttggatta attagctatt ttacagcggg agtaaaagag gtaagagctt
      901 ggactattga aaacggctgg aaggcgcctc gggctgcatc ggttatccat aatgactttg
      961 aaagaggttt tatacgtgct gaagttatca gctatgatga ttttattgaa tataagggtg
     1021 aagcaggagc caaggcagca ggaaaaatgc gaaccgaagg aaaagagtac agagtagcag
     1081 atggagacat catgcacttt ttattcaaca tataggactt acaaatcttt gtctttaaaa
     1141 agatttgtaa gtccggtttt cgagcgatag ctcgaaaaat attccatact tcttacaaat
     1201 ctttgtcttt aaaaagattt ttaagtctta cttttcgagc gatagctcga aaaatattcc
     1261 atacttctta caaatctttg tcttaacaaa gtttatacac tcccaaaaca ttaccagcga
     1321 catataattt gtgcaaaaat atgatgagac ataatatttt ctgaaattat tttttgctta
     1381 taatttttga taatatcttt gtctgtaaca aaaaagaatt gattatgtcc tatcatttgt
     1441 tattataaca taagactaaa ctcttggagc ctgcttttcc ccttttttat tgaaaaacaa
     1501 tcaccttttt tggaggcaaa atggaagctc agccagaaga tttgcatgaa gcagtgctgt
     1561 tgtatacaca tacaaaaatc gaacttgcca agcgtataaa agagggtata agttatacag
     1621 aatttttaga ttatgaaaaa aaacttgaac agattgatcg aattttaaaa aaagctgagc
     1681 ggcaacttga aaaagaggtt tttactggct cgagttgaat ttattgttat attttgccta
     1741 aaatttcatt tttgagcatc attttttgtg ggaaaaaatt tttgcgatgt ttttattaag
     1801 agatttgtta ttttactttg taaaagtttc atttgtgtat attttcagtt atttatttcc
     1861 gggaatcccc gatttaattg accgcataag atgtaataaa atattatcct gttactcctt
     1921 ttaaaaaggg gtttttaaaa ccggcttata attttattct gtacaggaat aagctttttt
     1981 gttatttggt tgtgaatgta gtaacaagca gggaagttat aaacttttga attctttaaa
     2041 aataatgact ttcgtctttt cttttctttt atttttagga gtaaatgttt atattctttt
     2101 ctaaaaaaga ctacataatg gcaattttat aataattgtt taaaaaaaat taaatacttt
     2161 aggagagtgt cgtaatgggt aagtcgatga caatggatgg aaacatggct gctgcacatg
     2221 tttcttacgc gtttaccgaa gttgcggcta tttatccaat aacaccgtca tctaatatgg
     2281 cggaatatac ggatgaatgg gcggctcacg ggaaaaaaaa tatttttggt gagcctgtca
     2341 ggctgataga aatgcagtca gaagggggag ccgcaggagc tgttcatggc tctcttcagg
     2401 caggagcttt gactacaacc tacacagctt cgcagggatt gcttttaatg attccgaata
     2461 tgtttaaaat ttctggggaa cttcttccag gtgtttttca tgtaagtgca agaagtgttg
     2521 ctgcacatgc actttcaatt tttggagatc attcagatat ctattctgca cgtcagactg
     2581 gatttgctat gttggcaact ggttctgtac aggaagttat ggatcttggt gctgtagcac
     2641 accttgctgc aataaagact agagttccat ttgttcactt ttttgacggt tttagaactt
     2701 cacacgagat tcagaaaatt gatacgtggg attatgctga cctagagaaa cttgtcgata
     2761 aagaggctct tcagaaattc agagataatg ctcttaatcc aaacaaccct gtaacacgtg
     2821 gtacagctca gaacccagat atctttttcc aggctcgtga agcttgtaat aagttttatg
     2881 atgcagttcc tgatgttgtt gcggactaca tgaagcagat ttctaaactt actggacgtg
     2941 aatataagcc gtttatgtat tatggagacc caaaagctga aaatatcatt attgcaatgg
     3001 gttcagtttc agataccatt aaagagacaa tcgattattt gaataagaaa ggtgaaaaag
     3061 ttggtgcaat aattgttcat ctttacaaac ctttttccgt aaaacatttg atggaagttc
     3121 ttccaaaatc ggttaagaaa attgctgtac ttgatagaac caaagagccg ggtgcagaag
     3181 gtgagccact ttatcttgat gttaaatcaa tattttatgg aaagaaagat gcaccggtta
     3241 ttgttggcgg tcgttatggt ctttcttcga aagatactac tccatcgcag ataattgctg
     3301 tttatgagaa tcttaagcaa aaagagtcta aagacggatt tactatcggt atagttgatg
     3361 atgtgacatt taaatcgctt ccacttactg cggaagtaaa tgtcgttcct gaatctacca
     3421 aacagtgtat gttttacggt ctcggatctg atggaactgt tggtgcaaat aagaattcta
     3481 tcaaaattat tggtgatcat acagatatgt atgctcaagg atattttgcg tatgattcaa
     3541 agaaatcagg tggaattact atttctcacc ttcgttttgg aaaagataaa attcgatcac
     3601 cgtatctggt taatcatgca gattttgttg cctgttctca accatcgtat cttgataaat
     3661 atgatatgct taagggtata aaaaaaggtg gtgcttttct tctaaattca atttggagtg
     3721 ctgaagagac aaaagctaat cttccagaca aggttaaaaa gattcttgcc gaaaatgaag
     3781 ttaacttcta tataatcaat gctacaaaaa ttgctgaaga gattggccta ggtggtcgca
     3841 caaatacaat acttcagtct gccttcttca aacttgcgga agttattcct tacgatgatg
     3901 ctgttaagta tatcaaggaa gctatcaaaa agtcttacgg taaaaaaggc gatgatatag
     3961 taaatatgaa caatgccgca gttgacaagg gtgctagtga acttgtcaaa gtcgaagtac
     4021 ctgctgaatg ggcttcaata aaaattgccg ctgacgcaac aagtgataga ccggatttta
     4081 taaaaaatat tgctgatgta attaatggtc aggatggaga ctcgcttcct gtgagtgcct
     4141 ttattggcgc ggaagacgga actttcccag ccggtacaac agcttatgaa aagcgtggta
     4201 ttgctgtaaa tgtgcctgaa tggcagagtg aaaattgtat tcagtgtaat caatgtgcct
     4261 ttgtttgtcc tcatgctgta attcgtccac ttcttatcga taaagatgaa aaggcaaaac
     4321 ttccatctga tattacaact atcaaggctg tgggaaaagg tgttgaagat tatgagtaca
     4381 agcttcagat ttcgccactt gactgtactg gatgcggaaa ctgtgctgat gtatgtccgg
     4441 ctccgaaggg aaaagcactc gttatgaagc ctatcgcttc acaggcaaaa gaggctgaac
     4501 tttgggatgt agttacaaaa aatgtaagct ataaaacaga taagtttcct cttactaatg
     4561 tcaagggatc gcagtttgct caaccgctct ttgaatttca cggagcttgt gccggatgtg
     4621 gagaaacacc atatattaag cttataactc agctttatgg tgaccgtatg atgattgcta
     4681 atgctacggg ttgttcatcg atttacggtg gttctgctcc agcaactcca tattgcaaaa
     4741 atgctaatgg ttgtggacct gcttgggcaa actcactctt tgaagataat gccgaatatg
     4801 gtcttggtat gtatcttggt gtttgcaaca tgcgtgatcg tatagaaaga cttatgaaag
     4861 aagctttggc ttctggagta tctggtgatg ttgaagctgc ttttaaagcc tggatagaaa
     4921 acaaagagaa cggcgaaact acaagagaag tttctacaaa ggtagaagag gtgcttgctg
     4981 gtaaatcgga tgcctatgca aaagaaatta tggctattaa acagt
//
LOCUS       DAOTGZ010000201         5003 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_3064
            NODE_19937_length_5003_cov_4.232619, whole genome shotgun sequence.
ACCESSION   DAOTGZ010000201 DAOTGZ010000000
VERSION     DAOTGZ010000201.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868088
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 5003)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5003)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:22:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,785
            CDSs (total)                      :: 2,751
            Genes (coding)                    :: 2,732
            CDSs (with protein)               :: 2,732
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 12 of 19
            Pseudo Genes (internal stop)      :: 5 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5003
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_19937_length_5003_cov_4.232619"
                     /isolate="AMR_MDS_3064"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..607
                     /locus_tag="P5123_11250"
     CDS             <1..607
                     /locus_tag="P5123_11250"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRX16881.1"
                     /translation="NFGRRIQQGFDQMDTWIDEGLSESAEHFVLNQPGYSRIDWFKND
                     IDEDIRNGASLCSWDQKISNYALSYTFMQYLRIHAKSGHSLYKKIINYSNGSYSGLAS
                     VMENENPYFINFKTIMLAYRMANLLQQEDNIYGYKSENSIFNLDVKPPTADLETLNLK
                     PGGSIAITTDKISVEQAAGSGRGSNIKLTTVVGGLVIELEK"
     gene            591..1025
                     /locus_tag="P5123_11255"
     CDS             591..1025
                     /locus_tag="P5123_11255"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRX16882.1"
                     /translation="MNWKNKKKTTLLIMTLILLFPLFCMARGANLRTIKTLNRNKLVS
                     VTPRMITSGKSYKIIGRIRLVGSERFQKIVVTTDGELTVELDVSKANKEKILEYQNRY
                     IEVEGVITSKKNRLADESGRGMSYKMKVKTYTILIVKDRDDL"
     gene            complement(1032..2405)
                     /locus_tag="P5123_11260"
     CDS             complement(1032..2405)
                     /locus_tag="P5123_11260"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: protein motif:HMM:NF024642.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent helicase"
                     /protein_id="HRX16883.1"
                     /translation="MEQLNAAQAKAAAFSRRHSLIIAGAGTGKTSTLIAKTAGIITDG
                     CVEPCRVALLTFSKKAAEEMRERLRKRLDKHADSMFVGTYHAFCLKAVRLHEEVFKNV
                     FGYMKFPSLLTSKQEMSINEKFYNDYGKCLCGLPFDIIPTLCENIYSGKISEYDLVKR
                     AGEDLLIAVKHYSKFRSEEKRKIGAFEFDDLITWCNLLYKKNRDFRTWVATRFDYLLV
                     DEFQDTSRNNFLMINNMLSDGATLCAVGDDYQSIYGFRKASPGIMIGFKKYLKDFDRF
                     MLTVNYRSTMEIVTVSNRLIRKNRNQIHKKMVSNMGSGGMVNLGFVSSFDDECRFIAD
                     IINHCDKKFSLAVLYRNNAYGNKVRERLSDICFNDNVSFSTFHSSKGLEYDHVIICGL
                     HNSQVPSPLNCLEEERRLMYVAITRARLRLDVISFAGDRRPLFVDELGIKRQWSKFIP
                     DYIIRDK"
     gene            2661..3857
                     /locus_tag="P5123_11265"
     CDS             2661..3857
                     /locus_tag="P5123_11265"
                     /inference="COORDINATES: protein motif:HMM:TIGR00707.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate aminotransferase family protein"
                     /protein_id="HRX16884.1"
                     /translation="MNNLENVMKLDREYLFQNYGNRLPVEFVRGEDSFLFDSTGKKYI
                     DFLSGISVVNLGYSNKHYTDTLHKQIDQIMHTSNWFFNSNQVQAAKQISDHSFKGRTL
                     FVNSGTEANEAAIKLARKYGQSIDEECYTILTFSSSFHGRTFGAMSATGNEKIRTGFG
                     PVVPGFKYVPFNDKDAFEEAVKQEKVCAVMVEMIQGEGGIKVADKDFIQHVYKIAKDN
                     GILFIADEIQTGIGRTGKLFAFEHYDIVPDIITLAKGLANGIPVGAMHAKEELLSLLG
                     AGTHGTTFGGNQLATAAVCAVFDIITKPSFLEHIHDMSKVVFDRLENIKKSVPLIKEI
                     RGKGLHIGVELESDGMPFVKKALDAGLVINCTAQNVLRIMPPLIIDENTLIQGLDIFE
                     KIVKQG"
     gene            3863..4807
                     /gene="argF"
                     /locus_tag="P5123_11270"
     CDS             3863..4807
                     /gene="argF"
                     /locus_tag="P5123_11270"
                     /EC_number="2.1.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014278406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004585 - ornithine carbamoyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0042450 - arginine biosynthetic process via
                     ornithine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine carbamoyltransferase"
                     /protein_id="HRX16885.1"
                     /translation="MKIPSVNTRHLLKVTDFSSEEIINLFSLAQSLKAAQKKGKKHHL
                     LKGKVLGMIFEKSSTRTRVSFETGIYHLGGTGLFLGKNDLQIGRGEPISDTAAVLSRY
                     LDGIMIRTFEHSKAEDFAKYGSIPVINGLTDDYHPCQALTDYFTMFEKLSKFKGTKLA
                     YIGDGNNMAHSLMLCGAMLGVNVSIASPADYAPNQGITDEARSIAAKNGSEIVITESM
                     DEAAKNADVLYTDVWASMGQEDEAEKRAKSFDGYCISIDLIKKNAPEAYVMHCLPAHR
                     GEEITAEVLDSDHSIIFDEAENRLHVQKAIMTVLMNSR"
     gene            4835..>5003
                     /locus_tag="P5123_11275"
     CDS             4835..>5003
                     /locus_tag="P5123_11275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015414134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004055 - argininosuccinate synthase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006526 - arginine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="argininosuccinate synthase"
                     /protein_id="HRX16886.1"
                     /translation="MGKINKVVLAYSGGLDTSVIVKWLIDTYQCEVVCYAADVGQEEE
                     LDGLDEKARKTG"
BASE COUNT         1657 a          960 c          939 g         1447 t
ORIGIN      
        1 aaacttcggt cgccgcatac aacaaggatt cgaccagatg gacacttgga ttgatgaagg
       61 tctttctgaa tccgcagaac attttgtttt aaaccaaccc ggttacagcc gaattgattg
      121 gtttaaaaac gacattgacg aagatatccg aaatggcgca tctttatgtt cttgggatca
      181 aaaaatcagc aactatgcgc tgtcttatac ttttatgcaa tacttaagaa tacatgctaa
      241 aagcggccat tcattatata aaaaaataat caattacagc aacggctcat attcaggtct
      301 tgcttctgta atggaaaatg aaaatccata ctttattaat tttaaaacta ttatgctagc
      361 ataccggatg gcaaatttac ttcaacaaga agataatata tatggttata aatccgaaaa
      421 cagtattttt aacctagacg ttaaacctcc aactgctgat ttagagactt taaatctaaa
      481 acccggcggt tcaatcgcta ttacaacaga caaaatatct gttgaacaag cagctggaag
      541 cggaagaggt tctaacataa aattaacaac agtggtagga ggacttgtaa ttgaattgga
      601 aaaataagaa aaaaacgaca cttctcatca tgacactaat cttgctattt ccactttttt
      661 gtatggcaag aggagctaat ttgcggacta ttaagacatt gaacagaaac aaacttgtct
      721 cggttactcc aagaatgatt actagtggta aaagctataa aatcatcggt cgtatacgct
      781 tggttggtag cgaacgtttt caaaaaatcg ttgtaacaac agatggcgag ttaacagtag
      841 agcttgatgt gtcaaaagca aacaaagaaa aaatactgga gtatcagaat cgctatatcg
      901 aagttgaagg cgttataaca tctaaaaaaa acagacttgc cgatgaatca ggtcgtggca
      961 tgagctataa aatgaaagtt aaaacttaca ctattcttat agtaaaagac cgcgatgact
     1021 tataattagc attatttatc cctgattatg taatcaggga taaattttga ccactgcctt
     1081 tttatcccca gctcgtccac aaaaagagga cgtctgtctc ctgcgaagct tattacatca
     1141 agccttaaac gagctctggt aatagcgaca tacatcagcc ttctctcctc ttcaagacaa
     1201 ttgagcggag aaggaacctg agaattatgt aacccacata taatcacatg atcatactca
     1261 agcccttttg aagagtggaa tgtcgaaaaa cttacattat cgttaaagca tatatcggac
     1321 agccgttctc gaaccttgtt gccataagca ttattacgat aaagtacagc aagactgaat
     1381 tttttatcac aatggttaat tatatctgca ataaaacggc actcgtcatc aaaactgctc
     1441 acgaaaccaa gatttaccat cccaccggaa cccatattcg aaaccatttt tttgtgaatc
     1501 tggtttctat ttttacgtat aagcctgttc gaaaccgtaa caatttccat ggtagaacga
     1561 tagttcacag ttagcataaa acgatcgaaa tccttaaggt actttttaaa ccctatcatt
     1621 attcccggag aagcctttct aaatccatat attgattgat aatcatcacc tacagcacaa
     1681 agggttgctc catctgacag catattattt atcatcaaaa aattatttct ggatgtatcc
     1741 tgaaactcat ctacaagcag ataatcaaac ctggttgcaa cccatgttct aaaatctctg
     1801 ttttttttat aaagcagatt acaccatgta atcaaatcat caaattcgaa agctcctatt
     1861 tttcttttct cttcacttct gaacttcgaa tagtgcttaa cagcaattag caggtcttct
     1921 ccagctcgtt ttaccagatc atactcacta atctttccgg aatagatatt ttcacaaaga
     1981 gtgggtataa tatcgaatgg caaaccgcaa aggcattttc cataatcatt ataaaatttt
     2041 tcattaatgg acatctcctg ttttgatgtt aaaagcgaag ggaatttcat atacccaaaa
     2101 acatttttaa acacttcctc atgcaaacgg acagccttta gacaaaacgc atgatatgtt
     2161 ccaacaaaca tactgtctgc atgcttatcc agccttttac gaagtctctc tcgcatctct
     2221 tcggccgcct ttttgctaaa agtcaaaagt gcaacacggc aaggctcaac acacccatca
     2281 gttattatac ctgctgtttt tgctatcaat gtagaagtct tgccggtgcc ggctcctgca
     2341 attattaaag aatgcctacg agaaaaggca gccgcctttg cctgtgccgc gtttaactgt
     2401 tccatggttg ctcctgctgt tatttaataa tttgtaaaag ttgtggtgct tagtattaca
     2461 gactgaattt ttaaaaaaaa gggcgatttt ttttgataaa aaacatcttt tctagacttt
     2521 ttatgttaaa aatcaaaata atatcagatt cagcaacttc attattgaca gaaaccgatc
     2581 cgattatgca caatgactat tttttatatg tttggataaa atattctttg caaattttag
     2641 tttattcaca ggaggcataa atgaacaact tagagaacgt tatgaagctt gacagggagt
     2701 acctttttca aaactacgga aaccgccttc ctgtagaatt tgttcgcggt gaagattctt
     2761 ttctttttga cagtacaggc aaaaaatata tagattttct ttcagggata tcagttgtaa
     2821 acctcggata cagtaataag cattacaccg acaccctgca taaacagata gaccagataa
     2881 tgcatacttc aaactggttt ttcaactcaa accaggttca agctgccaag cagatatctg
     2941 accactcatt taaaggacgc acgctttttg ttaacagcgg cacagaggct aacgaagctg
     3001 ctattaaact tgcccgcaaa tatgggcaat ctatcgatga ggagtgttat acgatattaa
     3061 cgttttccag ctcatttcat ggccgtacct tcggagccat gtcggcaacc ggaaacgaga
     3121 agataagaac aggctttggt ccggttgttc ctggatttaa atatgtacct tttaacgata
     3181 aagacgcttt tgaggaagca gttaaacagg aaaaagtttg tgcggtcatg gtcgaaatga
     3241 ttcaaggtga aggcggaata aaagtagccg acaaagattt tattcagcac gtttataaaa
     3301 ttgcaaagga taacggcata ctatttatag ctgacgaaat acaaacaggt attggcagaa
     3361 ccggtaagct ttttgcgttt gaacactacg acattgttcc tgatataatt actctcgcaa
     3421 aaggacttgc aaacggtata ccagttggcg ccatgcacgc aaaagaagag cttttaagcc
     3481 ttcttggtgc aggtacacat ggcacaactt tcggtggaaa ccagcttgct acagctgctg
     3541 tttgcgctgt ctttgatata ataaccaaac catctttttt agagcatatt catgatatgt
     3601 caaaagtagt ctttgataga cttgaaaata tcaagaaaag tgttcctcta attaaagaga
     3661 ttcgcggcaa agggcttcat attggtgttg aactagaatc tgacggaatg ccttttgtta
     3721 aaaaagctct tgatgcaggt ttggtaatca actgtacagc tcaaaatgtt cttcgaataa
     3781 tgcctccatt aataattgac gagaatacat taattcaggg actggatatt tttgaaaaaa
     3841 ttgtaaaaca gggttaatta ttatgaaaat tccttcggtt aacacaagac acctgctaaa
     3901 ggtgacagac ttttcgtcag aggagataat taatctgttc tctcttgctc agagcctaaa
     3961 agcagcacag aaaaaaggca agaaacacca cctgcttaag ggtaaggttc tgggtatgat
     4021 tttcgaaaaa agttctaccc gaacacgcgt atcttttgaa actggtattt atcatctagg
     4081 tggtacaggc ctttttttag gcaaaaatga tcttcagata ggtcgaggag aacctatttc
     4141 agatacagca gccgtccttt cgcgatatct tgacggaata atgatacgga catttgagca
     4201 cagcaaagct gaagattttg ccaaatacgg ctcgataccg gtaataaacg gtctaactga
     4261 tgattatcat ccatgtcagg ctcttactga ctattttaca atgttcgaaa aactgagcaa
     4321 atttaaaggc acaaaattgg cctatatagg cgacggcaac aatatggctc actctttaat
     4381 gctatgtgga gctatgcttg gcgtaaatgt ttccatcgcc tctcccgccg actatgcccc
     4441 aaatcaaggc ataacagacg aagcacgaag cattgcggcg aaaaacggat ctgaaatagt
     4501 aataacagaa tcaatggatg aggcagcaaa aaacgcagat gtcctttata ccgatgtctg
     4561 ggcaagcatg ggtcaagaag atgaggcgga aaaaagagcc aagagttttg atggctactg
     4621 tatatcaata gatcttatta aaaagaacgc acctgaagcc tatgttatgc attgtcttcc
     4681 ggctcataga ggtgaagaga taacagctga agttcttgat tcagatcatt ctataatatt
     4741 tgatgaagct gaaaacagat tgcatgttca aaaagcaata atgactgttc ttatgaactc
     4801 aagataatta ttatatataa caaggagaag cattatggga aaaataaata aagttgtttt
     4861 ggcctattcc ggcggattgg acacctcggt aattgtaaaa tggcttattg acacatacca
     4921 gtgcgaagtt gtatgctatg ctgccgatgt cgggcaggaa gaggaacttg acggtcttga
     4981 tgaaaaggcg cgcaaaacag gtg
//
LOCUS       DAOTGZ010000202         4933 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_3064
            NODE_20339_length_4933_cov_4.424764, whole genome shotgun sequence.
ACCESSION   DAOTGZ010000202 DAOTGZ010000000
VERSION     DAOTGZ010000202.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868088
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 4933)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4933)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:22:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,785
            CDSs (total)                      :: 2,751
            Genes (coding)                    :: 2,732
            CDSs (with protein)               :: 2,732
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 12 of 19
            Pseudo Genes (internal stop)      :: 5 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4933
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_20339_length_4933_cov_4.424764"
                     /isolate="AMR_MDS_3064"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1331)
                     /locus_tag="P5123_11280"
     CDS             complement(<1..1331)
                     /locus_tag="P5123_11280"
                     /inference="COORDINATES: protein motif:HMM:NF013998.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF58 domain-containing protein"
                     /protein_id="HRX16887.1"
                     /translation="MRNLISIIRNIYLERNFFIAAFVLPATAFAVFYFENLLSVFLIC
                     LSLFLGITIFELFFVIISSKKISARRILASRLSNGDDNQVTVEIRSDSNHSLVLRCID
                     ELPYQLQVRDWHFDTRVKPNSVEAWDYKIHPKKRGLYTFGSLHIYVKSFFRFIAYRVS
                     FETDQQVKVYPSIITLKEYDFYACSHKLPEPGLKRIRRSAVTHEFDALRNYRYGDEFR
                     SVNWKATARKGHLIVNNYVEDRSQQIYMVLDTGRSMQMPFEKMSLVDHAVNSALILSN
                     VAIKRQDKAGLITLSPTECSIVKADNRSNQISTILENLYKTENSNYEVNYELLYKTVN
                     NYIHHRSLLILYTNFETVHAMKRVLPILKLLSKRHLLLCVIFRNPILEKKIFIQAKTI
                     SDIYLSAAASDITDNKKIIYKELLQNGIQSIYVNHRSLTVQLINKYLELKAG"
     gene            complement(1328..2302)
                     /locus_tag="P5123_11285"
     CDS             complement(1328..2302)
                     /locus_tag="P5123_11285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013927605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MoxR family ATPase"
                     /protein_id="HRX16888.1"
                     /translation="MPANKTETITAEMASELLIKIKNNIAKAITGQSESVDLILTAMI
                     AKGHILLEGVPGVAKTLLAKTFAASLGLTYSRIQFTPDLMPSDITGTSVFNPKNLEFT
                     FQHGPVFAQTVLIDEINRSPAKTQAALFEAMQEQQVTADGSEYALPEPFLVIATQNPI
                     EYEGTYRLPEAQLDRFLFKIVVGYPSLDEETTILRNSHDRGFTKAEKNISSVISESEI
                     KQIRSLAEKAVVSEKLIEYTAQIIQATRSESKLYLGASPRAGVALLNGAKAYAVIQGR
                     DYVLPEDIQTILLPTLAHRVVLSPETEMEGQTIEDVLSIILKRIPVPR"
     gene            complement(2289..3497)
                     /locus_tag="P5123_11290"
     CDS             complement(2289..3497)
                     /locus_tag="P5123_11290"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRX16889.1"
                     /translation="MKIRLPKAYTLIITVLSLLFFITVFFAPVEQDWSDDFTYSQKKP
                     YGCFALYKLLIDNDLNNRLSVISKSVFDHNKEIFANNKDYNRIFITANANFDQYESET
                     VLAEIYKGATFFIAADYFTNSKIPDRMGFELTQLLFYNRTIEIYHNNDNSTYKFGKGI
                     KGSYISNFNPNTSTVIATTIDTEQKEPVAISIKYGKGKLILCSTPRIFTNYHLKENYR
                     LAFLLLGGLSVKTTFWDEYYKPGNVRQENILNFIDSDTMLSISFYGILVIVIIFALFG
                     AKRRERAIPVILPLKNSSLSFAKSIAALYLTQGSNIQIARKTVQNFYSHFYSQLPYAD
                     TNSIKKHDRQNISALCSQLSGMSTESCYKKICFIDSIEKSKNINSKELIFLYKTIIKL
                     LGKENKSAGK"
     gene            complement(3497..4177)
                     /locus_tag="P5123_11295"
     CDS             complement(3497..4177)
                     /locus_tag="P5123_11295"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRX16890.1"
                     /translation="MIKKIIFFIIFVVTSGVLFADTKIAIPEHDTVLELYASENLNYS
                     SQDRVTLIGSINRKIIEILRDFFRRLSSHDGFDLKEVYLIIIAVIIVLAVLFLLYKIL
                     KGVWFFYSPEKKVNAQISDIGLEVKQNTNYLHLYENALNNEDPDTAIRYLYLHALKDL
                     SDKNMIHLRKDKTNKIYTSEIKNIKAKNNFIKIANIFNSVYYGKNSASVKLALDVKKL
                     CDLLTEAE"
     gene            complement(4174..>4933)
                     /locus_tag="P5123_11300"
     CDS             complement(4174..>4933)
                     /locus_tag="P5123_11300"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRX16891.1"
                     /translation="LSETFNSSPANFIERINIPFLVVSIIIYLVAFMTIAAIFDTVLI
                     RFVALYKDTPYSSITDNAMKEQFKSWFLHVLKRNFQFILVVTLIYVIYFATLFSVRYI
                     PQMAITFIGVYMFYYIFALIWPILFIAYSTVVANEKLAFIKTLKRTFWLLQNNWLSAA
                     GLFYLTVMIAYVISIFMSIPSGIITGIGISLDINEATSKIGTTAIYCIAAILMILYYF
                     TYVVLPTLSFSIKYWQLREKKEGLSLKEAVDNVK"
BASE COUNT         1538 a          803 c          953 g         1639 t
ORIGIN      
        1 cctgctttaa gttccagata tttatttatc aattgcactg taaggcttct atgattaacg
       61 taaattgatt gtattccgtt ttgtaaaagc tctttataga tgattttttt gttatcagta
      121 atatcggaag cagcagctga gagatagata tcgctgatag tctttgcttg aataaatatt
      181 tttttttcaa gaatggggtt acggaatatt acacatagaa gaagatgtcg cttagatagc
      241 agtttcaaaa ttggcagtac tcttttcatt gcatgaacag tttcaaaatt ggtgtacaga
      301 atcagtaggc ttctatgatg aatataattg ttaaccgttt tgtacaagag ctcgtagttt
      361 acttcataat tgctattttc ggttttgtaa aggttttcaa gaattgtgga tatttgattt
      421 gagcgattat cggcttttac tattgagcat tctgttggag atagagtgat aagcccagct
      481 ttgtcttgtc gcttgattgc aacatttgaa agaataagtg ccgaatttac tgcatgatca
      541 acaaggctca ttttttcaaa cggcatttgc atagatcttc cggtgtcaag aaccatgtat
      601 atctgctgag aacgatcttc aacgtagtta ttgacaataa ggtgcccttt tcgagctgtt
      661 gctttccagt ttacagatct aaattcgtct ccgtaacgat agttgcgtaa tgcgtcaaat
      721 tcgtgtgtta ctgcacttct gcgaatccgt tttaacccgg gttcgggcag tttatggcta
      781 caggcataaa aatcgtactc tttcagcgta ataatagaag ggtaaacttt tacttgttga
      841 tctgtttcaa aagaaacacg gtaagcaata aagcgaaaaa aagatttcac ataaatatga
      901 agtgatccaa aagtataaag cccccttttt ttaggatgta ttttgtagtc ccaggcttca
      961 acgctgtttg gttttactcg tgtatcaaaa tgccagtctc ggacttgaag ctggtaaggt
     1021 agctcgtcaa tacatcgcag aacaagagag tggttgctat ctgatctgat ttcgactgta
     1081 acctggttgt catctccgtt agacaaacga cttgccagga tacgccgtgc tgatattttt
     1141 ttagatgata ttataacaaa aaaaagttca aatatagtta tacccaagaa aagggataaa
     1201 catataagaa aaactgataa aagattttca aaataaaaaa ctgcaaaagc cgtagcgggt
     1261 aatacaaatg ctgctataaa aaaattgcgt tccagatata tatttctaat aatactaatt
     1321 agatttctca tcttggtacc ggtattcttt ttagtattat agagagaaca tcttctatcg
     1381 tttgtccctc catctcggtc tctggtgaca ataccacacg gtgtgcaaga gttggcaaaa
     1441 gaatggtttg aatatcttcg ggtaacacat aatcgcggcc ctgaattacg gcataggctt
     1501 ttgctccgtt tagtagagcg acaccggctc ttggagaagc tccaaggtag agtttgcttt
     1561 cagagcgtgt agcttgtatt atctgagcag tatactcgat tagtttctct gataccactg
     1621 ccttttctgc aagagagcgg atctgcttta tctcggattc agaaataaca gaacttatat
     1681 ttttttctgc tttggtgaat ccgcgatcat gtgaatttct taaaatagta gtctcttcat
     1741 caagtgatgg ataaccaact acaattttaa acagaaaacg gtcaagttgt gcctcaggaa
     1801 gccggtaggt tccttcgtat tcaatagggt tctgggttgc tataaccaga aaaggttcgg
     1861 gtaaagcgta ttcgcttcca tcggcagtta cctgctgttc ttgcatagcc tcaaaaagtg
     1921 ccgcctgtgt tttagcgggt gagcggttga tctcgtcaat taaaactgtt tgtgcaaaaa
     1981 cagggccgtg ttgaaaggta aattcaaggt tttttgggtt aaaaaccgat gtgccggtaa
     2041 tgtcacttgg cataagatca ggggtaaact ggatacgcga gtaggttaat ccaagtgaag
     2101 cggcgaaagt ttttgcaaga agggttttag ctacaccggg tactccttca agcagtatgt
     2161 gccccttggc tatcatagcg gttaagataa ggtctaccga ttcactttga ccggtaattg
     2221 cttttgcaat attatttttg atttttatta gcagctccga agccatttca gctgtaattg
     2281 tttctgtctt atttgccggc acttttattc tcctttccaa gcagttttat aatagtttta
     2341 taaagaaaaa taagttcttt actgtttata tttttacttt tttcgatact gtcaataaaa
     2401 caaatctttt tatagcagct ctctgtactc atgccgctta gctgcgagca taaagcggaa
     2461 atgttttggc ggtcgtgttt cttgatgctg tttgtatcgg catatggtaa ttgcgaataa
     2521 aaatgagaat agaaattttg tacagttttt ctcgcgattt gtatattaga cccttgagta
     2581 agatataggg ccgctattga tttcgcaaaa cttagagagc tgttttttag aggcaatatt
     2641 actggtatag cgcgttctct tcttttagca ccgaacaacg caaatattat cacgataacc
     2701 agaatgccat aaaatgaaat tgataacatc gtatcagaat ctataaaatt gagaatattc
     2761 tcttgtcgca cattgccggg tttgtagtat tcatcccaaa aggttgtttt tacagatagc
     2821 ccccctaata gaagaaaggc cagtcgatag ttctctttaa ggtgatagtt ggtgaatatt
     2881 cgaggagtcg agcataatat taacttacct ttgccgtatt tgattgaaat tgctacaggc
     2941 tctttttgct ctgtatcaat ggttgttgca ataaccgtcg aagtatttgg attaaaattg
     3001 cttatatatg agcccttgat tccttttcca aatttataag tagaattatc attattgtga
     3061 tatatctcaa tcgtgcgatt atagaagagt agttgcgtta gttcaaaacc cattctatcg
     3121 ggtatttttg agtttgtaaa ataatccgcg gcaataaaaa aagtggctcc tttatatatc
     3181 tctgcgagta cagtttccga ttcgtactga tcaaaatttg cattagcagt gatgaatata
     3241 cgattgtaat ccttattatt ggcaaatatc tctttattat gatcgaacac agattttgaa
     3301 ataacgctta atctattatt aaggtcatta tcgataagta gtttataaag agcaaagcag
     3361 ccataaggtt ttttttgaga ataagtaaaa tcatcgctcc agtcttgttc aacaggagca
     3421 aaaaaaactg ttataaaaaa aagcagtgat aaaacggtaa taatcaaagt gtaggctttt
     3481 ggaagtcgaa ttttcattat tcggcctccg ttagtaaatc gcataacttt ttaacatcaa
     3541 gtgctagttt tactgacgcg ctgtttttcc cataatagac agagttaaaa atgttagcta
     3601 ttttaataaa attatttttt gctttaatgt ttttaatttc cgaggtgtaa attttgttgg
     3661 tcttgtcttt gcgcaaatga atcatgtttt tatctgacag atctttaagt gcatgcagat
     3721 aaagataacg aatcgctgta tcgggatctt cgttatttaa ggcattttcg taaagatgca
     3781 gataatttgt attttgttta acttccagac ctatgtcaga aatttgagca ttgacttttt
     3841 tttctggtga ataaaagaac cagacacctt ttaaaatttt atatagtaaa aaaaggactg
     3901 ctaaaacaat tataactgct atgattatga ggtacacctc ttttaagtca aaaccgtcgt
     3961 gagaagacaa tctgcgaaaa aaatctctca gaatttcgat tatttttcta tttatcgatc
     4021 caataagtgt gactcgatct tgagaactat agtttaaatt ttctgaagca tataactcaa
     4081 gaaccgtatc atgctcgggt atggctattt ttgtatccgc aaaaagtaca ccggatgtga
     4141 cgacgaaaat gataaaaaaa ataatttttt ttatcattta acgttatcaa cggcttcttt
     4201 aagtgataac ccctcttttt tttcacgtag ttgccaatat ttaatagaaa aagagagagt
     4261 gggtagcaca acataggtaa agtagtacag aatcataaga atagcagcga tgcagtatat
     4321 agcagtagtg cctatttttg atgttgcttc atttatatca agggaaattc caataccagt
     4381 tataatgccg gatggaatgg acataaaaat ggatattaca taagcgatca taacagttaa
     4441 atagaaaaga cctgctgccg ataaccaatt gttttgcaat aaccagaaag ttctttttaa
     4501 ggttttgata aatgctaatt tttcattggc gacaacggtg ctgtaagcaa taaaaagaat
     4561 cggccagatc agagcgaata tatagtaaaa catatagacg ccaatgaagg tgattgccat
     4621 ttgcggaata taccgaaccg aaaatagtgt agcaaaatag ataacataaa taagcgtaac
     4681 taccagtata aactgaaaat ttcttttcag aacatgtaaa aaccatgatt taaactgttc
     4741 tttcattgca ttatcggtaa ttgaagagta gggagtatct ttgtataatg caacaaatcg
     4801 tataagcact gtatcgaaga tggccgcaat agtcataaat gcaactaaat aaatgattat
     4861 agatacaaca agaaagggga tatttattcg ctctataaaa ttagctggag atgaattaaa
     4921 tgtttcagaa agg
//
LOCUS       DAOTGZ010000203         4927 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_3064
            NODE_20367_length_4927_cov_3.821634, whole genome shotgun sequence.
ACCESSION   DAOTGZ010000203 DAOTGZ010000000
VERSION     DAOTGZ010000203.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868088
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 4927)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4927)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:22:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,785
            CDSs (total)                      :: 2,751
            Genes (coding)                    :: 2,732
            CDSs (with protein)               :: 2,732
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 12 of 19
            Pseudo Genes (internal stop)      :: 5 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4927
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_20367_length_4927_cov_3.821634"
                     /isolate="AMR_MDS_3064"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..680
                     /locus_tag="P5123_11305"
     CDS             <1..680
                     /locus_tag="P5123_11305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002656189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="AIR synthase-related protein"
                     /protein_id="HRX16892.1"
                     /translation="EALYDMSTFFDIPMTSGKDSMKNDFKGGGKKISVPPTILYSMAA
                     KIMDVRHCVSSNFKATGDLVYVLGETYDELGGSEFYRLFNELGANVPVVRPELAKKLY
                     IAVSEANRESLIESSHDISDGGLAVCLAECCFGTSFGVAASIDSIATVGRNAAIFGES
                     HSRFVVSVAPQNRVRFEEHFGKRAFLLGNVTDNGKFTLSESGTCLIDSSVSDLLASWK
                     STLEIME"
     gene            677..1504
                     /locus_tag="P5123_11310"
     CDS             677..1504
                     /locus_tag="P5123_11310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006926924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase
                     subunit PurQ"
                     /protein_id="HRX16893.1"
                     /translation="MKKVRSLVITGFGINCEEEMAAAYTLAGAEAVIVHLNDILIDGF
                     SIHDFDILNFPGGFSFGDDIASGRVLANKIRFKKLPSGLTFIDEIKKFIKAGKFVLGV
                     CNGFQVLVKLGLLPDLSGEIKQEVTLTRNDSSKFEDRWCYCKRADNVRTPFLKDIDII
                     YLPVRHGEGKLMIADENVRKKILDNNLAVLSYCDSTGKITDKYPLNPNGSEMSIAGLT
                     DTTGQVFGLMPHPEAFLTLYNHPDWGRLRRENPSIPEDGEGLKIFKNIITHIASQER"
     gene            1506..2891
                     /locus_tag="P5123_11315"
     CDS             1506..2891
                     /locus_tag="P5123_11315"
                     /EC_number="6.3.2.6"
                     /inference="COORDINATES: protein motif:HMM:NF010568.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004639 -
                     phosphoribosylaminoimidazolesuccinocarboxamide synthase
                     activity [Evidence IEA];
                     GO_process: GO:0006164 - purine nucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylaminoimidazolesuccinocarboxamide
                     synthase"
                     /protein_id="HRX16894.1"
                     /translation="MKCLDTFSTPQLELIHKGKVRDSYRIDDKRRMIVVTDRISAFDN
                     VLNNYIPYKGAVLNGISNFWFENTMDIIDNHILEVVDPNINIVKEADPIRVEVIVRGY
                     LTGSMWRGYQNGKRTFSGVTVGEGLACNQKFPEPIVTPTTKEDSDREISPEDIAKEGW
                     VSSDIYQKMADISLKLFERGSSVLAQNGIVLVDTKYEFGLYKGKLILIDEIHTPDSSR
                     FWRASDYEKDPQSAEQIDKEFVRQWMIKNSKNGKYPDTLDDSVVEETSRRYVEIFEII
                     TGQKFDYNQFYDSKHRVAQNLCSAGLIKDGYIAIVMGSASDKTHADKMKSTIEKYDIK
                     CFYRVISAHKNGERLVQLGETLNNSVEPGAVIAVAGRSNGLGGALGANLSIPVISCPP
                     FKDNADIMVNVNSSLMMPSLTPASTVVYPDNAALNALRSLNIQRVRKQMTDEIDRMKK
                     DLIEADKNINK"
     gene            2988..4238
                     /gene="purD"
                     /locus_tag="P5123_11320"
     CDS             2988..4238
                     /gene="purD"
                     /locus_tag="P5123_11320"
                     /EC_number="6.3.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006926919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004637 - phosphoribosylamine-glycine
                     ligase activity [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylamine--glycine ligase"
                     /protein_id="HRX16895.1"
                     /translation="MNIVVIGSGGREHALCWKLKQSPLADEIFALPGNGGIPNSVDID
                     PFDFQAVEDFCKENDISLIVVGPENPLAAGITDYFTKKDIMIFGPDKAGAALEGSKIF
                     AKEFMLKYGVATAGYHKITSSDQAEEVIDKYNGSCVLKYDGLAAGKGVYVCCSKEESS
                     QAVDELLATYGNDANYIIEELLVGDEISIIGITDGKSIKLLSPSQDHKQLCENDEGPN
                     TGGMGAFTPVPFANDKLLESIDNDIVKPTLHGIIKEGYNFKGIVYFGIMVTKNGPKLL
                     EYNVRFGDPETEVLLPALKSDLLEIFLSCFDNTLDQKKVEFNEGYFVDVVLAAGGYPR
                     EYNKGDTISGLDQVSEDTLVFHAGTIKKDGNIITNGGRVLNVVCGGKSLDDAISKVYS
                     ECSKISFDNCYIRKDIGRREQIVK"
     gene            4329..4904
                     /gene="purN"
                     /locus_tag="P5123_11325"
     CDS             4329..4904
                     /gene="purN"
                     /locus_tag="P5123_11325"
                     /EC_number="2.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006926918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004644 - phosphoribosylglycinamide
                     formyltransferase activity [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylglycinamide formyltransferase"
                     /protein_id="HRX16896.1"
                     /translation="MKNIVIFISGRGSNMEAILNNIESGILKGVCNVLCVVSNRADAA
                     GLDTARSYGVKTAVVISRGVSPEEYNNNLSELIDEYNPDYIVLAGYMKILDPDFVKRY
                     PKRIINIHPADTSAHQGLHAYEWAFENRLKESFITVHYVDEGVDTGEVIGKASVDMGG
                     ANSLEEYEKRGLAVEHSFYSEMLKKVFTGII"
BASE COUNT         1504 a          801 c         1142 g         1480 t
ORIGIN      
        1 gtgaggcact ttatgatatg agtacctttt tcgatattcc aatgacatcc ggcaaagact
       61 cgatgaagaa cgattttaaa ggcggcggta aaaagatttc tgtgccgcca acaatactat
      121 actccatggc agcaaaaatt atggatgtgc gccattgtgt gagctcaaac ttcaaggcga
      181 cgggtgacct ggtttatgtt ttgggtgaaa catacgatga gcttggtggc tcggaatttt
      241 acagattatt taacgagctt ggagccaatg tcccggttgt tcgacccgaa cttgcaaaaa
      301 aactgtatat tgctgtatct gaagctaacc gtgaatccct gatcgagtct tcgcatgata
      361 tttcagatgg tggccttgcg gtatgtttgg ccgagtgctg tttcggaact agttttggtg
      421 ttgcagcctc tattgattca atcgcaactg tcggacgaaa cgcggcaatt ttcggtgagt
      481 cccattcaag atttgttgta tcggtggcac cacaaaacag agtacggttt gaagaacatt
      541 ttggtaagcg tgcgtttctg ctaggaaatg ttaccgacaa cggaaaattt acccttagcg
      601 aaagtggcac ttgcctgatt gattcatctg taagtgatct tcttgcttca tggaaaagta
      661 cactggagat aatggaatga aaaaagtacg ctcgcttgta ataacaggtt ttggaataaa
      721 ttgtgaagaa gagatggctg cggcctatac tctggccgga gccgaagctg taatcgttca
      781 tcttaatgat attctgatag atggattttc gatacacgat tttgatatac ttaattttcc
      841 ggggggattt tcgtttggtg acgatatagc ttcgggaagg gtgctcgcaa acaagatcag
      901 gtttaagaaa cttccttcgg gattaacttt tattgatgaa attaaaaaat tcattaaagc
      961 tggtaagttt gttttgggcg tgtgtaacgg tttccaggtt cttgttaaac ttggtctttt
     1021 gccggatctt tcgggagaga taaagcagga agttacctta actcgtaatg attcttcaaa
     1081 atttgaagat agatggtgtt actgtaaaag ggctgataat gttagaactc cgtttttaaa
     1141 ggatattgat atcatatatc ttcccgtgcg tcacggtgaa ggaaaactta tgatagcaga
     1201 tgaaaatgta cgaaaaaaga ttttggataa taatctcgcg gttttatcat attgcgactc
     1261 tactggaaaa attactgata aatatccgtt aaaccctaat ggatccgaaa tgagtatcgc
     1321 cggattaact gatactactg gacaggtttt tggacttatg ccccatccgg aagccttttt
     1381 gactttatat aaccatcccg attgggggcg cctgcgacgt gaaaatccgt cgataccaga
     1441 ggatggtgaa gggcttaaaa tatttaaaaa tataataacg catatcgcgt cacaggagag
     1501 ataagatgaa gtgtcttgat actttttcga caccccagct tgaacttatt cacaaaggta
     1561 aggtacgtga cagttaccgg atcgatgaca aacgtcgaat gatagttgta accgatcgta
     1621 tatccgcgtt tgacaatgtt ttaaataatt atatcccgta caaaggtgca gtgcttaatg
     1681 gaatatccaa cttctggttc gaaaacacaa tggatattat agataatcat atactagaag
     1741 tcgttgaccc aaatattaat atcgtaaaag aggctgatcc tattcgtgta gaagtaattg
     1801 tcagaggtta tctgacaggc tcgatgtggc gcggatatca aaatggaaag agaaccttta
     1861 gtggtgtaac tgttggagaa gggcttgcct gtaatcagaa atttcctgaa ccgattgtga
     1921 ctcccactac aaaggaagat tctgaccgtg aaatatcacc agaggatatt gccaaagaag
     1981 gttgggtaag ttcggatatc tatcaaaaaa tggccgatat ctctttaaaa ctatttgaaa
     2041 ggggaagttc tgttcttgct caaaatggaa tagttctggt tgatacaaaa tatgaattcg
     2101 gtttatataa aggtaagctt atactgatag acgagataca tacaccggat tcatcacgtt
     2161 tttggagagc ttcagattat gagaaagatc ctcagtcggc tgaacagatt gacaaggagt
     2221 ttgtgcgtca atggatgata aaaaacagta aaaacggaaa atatcctgat actcttgacg
     2281 atagtgttgt cgaagagaca agccgccgtt atgttgagat attcgaaata ataacaggtc
     2341 aaaagtttga ttacaatcag ttttatgatt caaaacatcg ggtagcacaa aatctttgca
     2401 gtgccggact gataaaggat ggctatatag cgattgtaat ggggtctgct tcagataaaa
     2461 cccacgcgga caagatgaaa tcaaccatcg aaaaatacga tattaaatgt ttttaccgag
     2521 taatatcggc acataaaaat ggtgagcgtc ttgtgcagtt aggtgagaca ttgaataatt
     2581 cggtagagcc cggtgccgtt attgccgtag ccggacggtc taatggattg ggaggcgcgt
     2641 tgggcgcaaa tttatccata cctgttatta gctgtccgcc atttaaggat aacgccgata
     2701 taatggttaa tgtaaattca tctcttatga tgccgtccct gactccggca tctactgttg
     2761 tttatcccga caatgctgca ttgaatgctt tgcgttcttt gaacattcag cgtgtgcgaa
     2821 agcagatgac tgatgaaatt gataggatga aaaaagattt aattgaagca gataagaata
     2881 taaataaata gttgtacagg attatcagag gcgctttaaa atcagtatct gcagggtggt
     2941 taatcacagg tttttaaagc gctatttttt attttaagga ttgatagatg aatattgttg
     3001 taatcggttc gggcggccgt gaacatgccc tctgctggaa actgaaacag tcacctttgg
     3061 cggatgaaat attcgcattg ccgggaaacg gtgggatacc aaatagtgtt gatatagatc
     3121 ctttcgactt tcaggccgtc gaagatttct gtaaagaaaa tgatatttca ctaatcgttg
     3181 tcggtcctga aaatcctctt gcagctggta ttaccgacta ttttacaaaa aaagatatca
     3241 tgatatttgg acctgacaag gcgggagccg ctctagaagg ttcaaaaata ttcgcaaaag
     3301 aatttatgct aaaatacgga gttgctaccg ccggctatca caagattaca tcttccgatc
     3361 aggcagaaga agttatcgac aaatataacg gtagctgtgt gctaaagtat gatggtcttg
     3421 ccgctggtaa gggggtttat gtatgctgtt ccaaagagga gtcgtcacaa gctgttgatg
     3481 agttgctggc cacttatggc aatgatgcaa attatataat tgaagagtta cttgttggag
     3541 atgaaatatc tattattggt ataactgacg gtaagtccat taaacttctt tctccatcgc
     3601 aagatcacaa gcaactttgt gaaaacgatg aggggccaaa cactggagga atgggagcct
     3661 ttactcccgt tccctttgcc aatgacaagc ttcttgaatc aattgataat gatattgtga
     3721 agccgacttt acatggtatt attaaagagg gttataactt caagggaatc gtctattttg
     3781 gtataatggt tacaaaaaat ggaccaaaat tgttagagta taatgtgcga tttggcgatc
     3841 ctgaaaccga agtactttta cctgccttga aaagtgatct tctagaaatt tttctgagct
     3901 gttttgacaa cactcttgat cagaaaaagg ttgagtttaa cgaaggatat tttgtagatg
     3961 ttgtattggc agcgggcggt tatccccgtg aatataataa aggcgatact atttcagggc
     4021 ttgatcaggt atctgaagat acgctagtgt ttcatgcagg aacaattaaa aaagatggta
     4081 acatcataac taatggtgga cgtgttttga atgttgtttg tggcggaaag agtcttgatg
     4141 atgcaatatc gaaggtttac agtgaatgct caaaaataag ctttgataac tgttacataa
     4201 gaaaagatat tggccgacgt gaacagattg taaaatagtt gtcttttttt aaataattat
     4261 ctcttaaatg ttgaataatg aatcgaatgg ctttagctgg tcaaataata ttgtgtagtc
     4321 ggtaaaaaat gaagaatatt gttatattta tatcaggtcg cggatctaat atggaagcga
     4381 ttctgaataa tattgaatct ggtatattaa agggtgtctg taatgtgcta tgcgttgttt
     4441 ctaatcgtgc agatgctgca gggcttgaca cggcacgttc atacggagtg aaaacagctg
     4501 ttgttatttc tagaggggtt tctcctgaag agtataataa taacctgtcg gagttgattg
     4561 atgaatataa tcccgattac atagtgcttg ccggatatat gaaaatattg gatcccgatt
     4621 ttgtaaaacg ttatccgaaa cgaattataa atattcatcc tgctgatact tctgctcatc
     4681 aaggtttgca tgcctatgaa tgggcattcg aaaacagact taaagagagt tttataacag
     4741 ttcactacgt agatgagggt gtggacacag gtgaggtaat tggtaaggcc tctgtcgata
     4801 tggggggggc taattctctt gaagagtatg aaaaacgtgg gcttgcagtt gagcacagtt
     4861 tttacagtga aatgttaaaa aaggtattta cgggtataat ataagcggct aacaagccgc
     4921 tttttat
//
LOCUS       DAOTGZ010000204         4916 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_3064
            NODE_20417_length_4916_cov_4.201605, whole genome shotgun sequence.
ACCESSION   DAOTGZ010000204 DAOTGZ010000000
VERSION     DAOTGZ010000204.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868088
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 4916)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4916)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:22:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,785
            CDSs (total)                      :: 2,751
            Genes (coding)                    :: 2,732
            CDSs (with protein)               :: 2,732
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 12 of 19
            Pseudo Genes (internal stop)      :: 5 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4916
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_20417_length_4916_cov_4.201605"
                     /isolate="AMR_MDS_3064"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..180
                     /locus_tag="P5123_11330"
     CDS             <1..180
                     /locus_tag="P5123_11330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002692300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AbrB/MazE/SpoVT family DNA-binding
                     domain-containing protein"
                     /protein_id="HRX16897.1"
                     /translation="VKLPKEFQFSGKDVFIQKVGDAVILLPHDKSWEIFLHGLNNFTE
                     DFMNEECDQGKDQER"
     gene            170..304
                     /locus_tag="P5123_11335"
                     /pseudo
     CDS             170..304
                     /locus_tag="P5123_11335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002616824.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="VapC toxin family PIN domain ribonuclease"
     gene            394..921
                     /locus_tag="P5123_11340"
     CDS             394..921
                     /locus_tag="P5123_11340"
                     /inference="COORDINATES: protein motif:HMM:NF013077.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HRX16898.1"
                     /translation="MNVIEAIFARHSYRGKYKQQCIPKEDLITILKAGLAAPSGCNKQ
                     TTSLIAVEDVKVLEKLKSVIDPPMADTAPAMICVLTQRINAYRDKCFAIHDYSAAIEN
                     MLLAIVELGYQSCWIEGHITDQDKIGDKMAKILGVPDDYELVCFLPIGIAETEPSLPL
                     KKEFSQRAWFNKFGE"
     gene            1024..2049
                     /locus_tag="P5123_11345"
     CDS             1024..2049
                     /locus_tag="P5123_11345"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004763437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine protein kinase"
                     /protein_id="HRX16899.1"
                     /translation="MGDYFYELTPEPVFRAIEKGGFNPTGHIMALNSYENRVYDLKLE
                     DGSHIVSKFYRPGRWNEEQIREEHSFLLEIADAEVPVCAPMRFPDGDTLHSVNGIFYA
                     VWRRTGGRSAHDLSDTDIQIIGRMLACIHNVGASKNIQSRHKLDAKSYALDPLEYILQ
                     NNLLPSHCRDRFEKAVHQVARIYTELSENVPFHRIHADCHPGNLLNGTDGWFILDFDD
                     FCSGPAVQDIWMLCGGNDSYGKRHREILIEAYETFRPFNRSWFNLIEPLRALRFIRYA
                     GWIAKRWKDPVFPIAFPHFGTEAYWETETNELEAQLRVIKEGSPDYQGSSEPEEELTN
                     SDFFYDM"
     gene            2067..2189
                     /locus_tag="P5123_11350"
                     /pseudo
     CDS             2067..2189
                     /locus_tag="P5123_11350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008463099.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="Fic family protein"
     gene            complement(2198..3430)
                     /locus_tag="P5123_11355"
     CDS             complement(2198..3430)
                     /locus_tag="P5123_11355"
                     /inference="COORDINATES: protein motif:HMM:NF015451.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HRX16900.1"
                     /translation="MGASNNYNTIVIGAGPAGLFCAGTLGSLKLSLPVLLIEKMKRPG
                     RKLLLSGAGSCNITNDSSIDQFITHYGKNGRFMKHALYSFSPTELIAFFESRNINFIT
                     DKNGKYFPSSKKASDILNSLTDYCRENGVGIQTSTSVTTIVKQNDLFIVQTADKSFTS
                     LNIVIATGGKSCPGTGSTGDGYLLAQSLGHTIIAPVPALASVVVDTYQFCELSGISFE
                     DVILSQNATKNRTRGGLLFTHTGISGPAVLHLSRYLSPKDTIYADLIPDEKEEETLEK
                     IKTAVNQNGRQKFKHALNFLPFSARFMLKLYQNLRIDPDIDAAQVSNKALHDIANTLH
                     NLEFKIRNIESFETCMATAGGISLKEISGKSFESKLVKGLYFIGETIDIDGDSGGYNL
                     QAAFSTAFCAARHISQID"
     gene            complement(3431..>4916)
                     /locus_tag="P5123_11360"
     CDS             complement(3431..>4916)
                     /locus_tag="P5123_11360"
                     /inference="COORDINATES: protein motif:HMM:NF013702.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HRX16901.1"
                     /translation="TETAPMISCPRPGKVKIGNVGYPITNTEVKIINDEICCRGRNIM
                     KGYYNRPEETAEVIYDGWLHTGDKGYFDRKGYLHVNGRIKEIIILSNGKNVNPVEIEQ
                     KLEKNSDLVKEAAVIEENDLLAAVIHPDSEYLQKQGILNIEEHIKQEVIDPYNRSASS
                     YKRIKKVILTSTDFPRTRLGKIQRFKLERTPAPTDKQSYHQTGNSKESTIIMDYILKE
                     TGRKVKVSDHIEIDVGLDSLDKLSLISFIQTRFNMDADEDILSKYPTIQAIADYIGIN
                     KNSGVSETGDEDPLELPKSRFPHITLRNLLCLPLRFLSRIKISGRENIPAGAYIICAN
                     HQSAFDWLFVAHSMHKSELRKSYIFAKVKHFKSALRRFLASRANIILLDVNRDLNASL
                     DNMQNVVKSGKNLVLFPEGTRSKDGSLLEFKKTFAVLSKEANVPVLPLAIDGAYKVLP
                     KSAFFIRPFRTVKVKYLEPVYPSGRSVEEVLSITRSRIEDALNN"
BASE COUNT         1378 a          974 c         1105 g         1459 t
ORIGIN      
        1 gttaaattac caaaagaatt tcaattttcg ggaaaagatg tttttatcca aaaagttgga
       61 gatgccgtaa tactactacc tcacgataaa tcctgggaaa tattccttca cgggttaaac
      121 aatttcactg aagactttat gaatgaagaa tgcgatcagg ggaaagacca agaaaggtaa
      181 aataattgga cccatggatt tattaatagc agctcaggct atatcaaaaa aactgatact
      241 cgttacaaat aatataaaag aatttgaaag agtagagaaa atcaaaattg agaattgggt
      301 ataaaattta aacgcagtat ccagtaactg atacaatact ggttaaaata tacagcacca
      361 aaaataagaa gtttagttgg gagagacggt ttgatgaatg ttatagaggc aatcttcgca
      421 agacatagct accgaggtaa atacaagcaa caatgtattc ccaaagaaga tttgattaca
      481 atattgaagg ccgggcttgc agcgccatcg ggatgcaata agcaaactac aagtctgata
      541 gcagtggaag acgtcaaggt gcttgaaaaa ttgaaaagtg taattgatcc gcctatggcc
      601 gatactgcac ctgcaatgat ttgcgtgctt actcaacgaa taaatgcgta cagggacaag
      661 tgttttgcaa ttcatgatta ttcggcggca atagaaaata tgctacttgc aatagttgaa
      721 ctggggtatc agagctgctg gattgagggg catattactg atcaagataa aatcggagac
      781 aagatggcaa agattttagg tgtaccggac gattacgaac tggtctgttt tctgccgata
      841 ggaattgccg aaacggaacc atcattgccg ttgaagaagg agttttcaca aagggcatgg
      901 tttaataaat ttggagaata gtaaggactg tttactatat atatgtgatc gctttcgcgt
      961 gctttctata agacaattga gaaaagtcgt tgccagtact ccgctgttct atagtattga
     1021 ctgatgggtg attattttta cgaattaact cccgaaccgg ttttccgtgc tattgaaaag
     1081 gggggattta atccgacggg gcacataatg gcccttaata gctacgaaaa ccgggtatac
     1141 gatctcaagc tagaagacgg ctctcacatc gtctctaaat tttaccgccc agggcggtgg
     1201 aacgaggagc agattcgtga agagcactct tttttgcttg agattgctga tgcagaggtg
     1261 cccgtttgtg cacctatgcg gtttccagac ggcgataccc ttcattctgt gaacggtata
     1321 ttttacgctg tctggcgccg caccggcggc cgctcagctc acgatctgtc ggataccgat
     1381 attcagataa tcggccgcat gcttgcctgc atacataatg tgggagcctc aaaaaacatt
     1441 cagtcacgtc acaaacttga cgctaaaagc tacgctcttg atccgctcga atatattcta
     1501 caaaataatt tattaccatc tcattgccgc gacagattcg aaaaggccgt acaccaggtc
     1561 gctcgtatat ataccgaact aagtgaaaac gttcctttcc accgtattca tgccgattgc
     1621 catcctggta atcttcttaa cggaactgat ggctggttta ttttggattt tgatgatttt
     1681 tgctccggcc ctgcagttca ggatatctgg atgctctgcg gtggtaatga ttcgtacgga
     1741 aaacgacacc gcgaaattct tatagaggct tacgagacgt ttaggccctt taaccggtca
     1801 tggtttaacc ttatagaacc tttacgagct ctcaggttta tccgttatgc tgggtggata
     1861 gcgaaacgtt ggaaagaccc tgttttccct attgcgtttc cccattttgg taccgaggcc
     1921 tactgggaaa ccgaaacaaa cgagctcgaa gcgcagctgc gcgtaatcaa ggaagggtca
     1981 ccagattatc aaggctcttc agaaccggaa gaagagttaa caaacagtga ttttttttat
     2041 gatatgtgat tgattcttct tccatgttgc attctgtagg gttggcagct gatgttcatg
     2101 aacgtttggc aaccattcac ccttttatcg ggggaaacgg acggacagca cgtcttgtaa
     2161 tggatattgc tctgatgcaa aaaggttatt tgtcggatta atcgatttga ctaatatgcc
     2221 tggctgcgca aaaagcggtt gagaaagcgg cctgtaaatt atagccaccg ctgtctccgt
     2281 caatatcgat tgtttctcca ataaaatata gtcccttaac aagttttgat tcgaagctct
     2341 ttccggatat ctctttcagg cttataccac ctgctgtggc catacaagtt tcaaaagact
     2401 cgatatttcg tattttaaat tcaagattgt gcagagtgtt ggctatatca tgtaatgctt
     2461 tatttgatac ttgtgcggcg tcaatatctg gatcaattcg caggttttgg taaagtttta
     2521 gcataaaccg agcgctaaag ggtaaaaaat taagagcgtg tttgaatttt tgtcggccgt
     2581 tttgatttac tgctgtttta attttttcaa gagtctcttc ttccttttcg tcgggaatca
     2641 gatcggcata tatcgtatct tttgggctta agtatcggga aagatgtagc accgcgggtc
     2701 ctgaaatacc ggtatgggta aagagcaatc cgccgcgggt tctgttcttt gttgcatttt
     2761 gtgaaagtat tacatcttca aacgagattc cgctaagctc acaaaactga taggtatcaa
     2821 caactaccga agcgagtgcc ggcaccggtg caataatggt gtggcctaac gattgagcca
     2881 gcaggtatcc gtcaccagtc gatccggttc ccggacaact ttttccacct gtggcaataa
     2941 caatatttag tgaggtaaaa gatttatcag cagtttgaac aatgaaaaga tcattctgtt
     3001 ttacaatggt tgtcacggaa gttgatgtct gtatacctac tccgttttca cgacaataat
     3061 cagttaatga gttaagtatg tcggatgctt ttttagaaga ggggaaatat tttccatttt
     3121 tgtctgttat aaaattgata tttctggact caaaaaaagc aataagctcg gtcggtgaaa
     3181 acgagtaaag ggcatgcttc ataaagcgac cgttttttcc gtaatgcgtg ataaactggt
     3241 cgatggaaga gtcgttggtt atgttgcagc tgccagctcc gcttaaaaga agcttgcggc
     3301 cgggtcgttt catcttttct atcaacagaa ccggtaagct gagcttaaga gaaccaagag
     3361 tgcctgcaca gaagaggccg gcaggaccag cccctattac aatggtattg taattattcg
     3421 atgcccccac ctaattgtta agtgcatctt ctatgcgtga acgtgttatg cttaatacct
     3481 cttcaacact tcttcccgag gggtacacgg gttcaagata tttgactttg actgttctga
     3541 aagggcgaat aaaaaaagcc gatttcggta atactttgta agccccgtca atggcaagag
     3601 gaagcactgg cacgttggcc tctttcgaaa gaacagcaaa tgtctttttg aactctagta
     3661 gcgaaccgtc tttgcttctt gtgccttcag gaaaaagaac caggtttttt ccgcttttaa
     3721 caacattttg catattgtca agcgaggcgt taagatcccg gttaacatca aggaggatga
     3781 tgtttgctcg tgaagcaaga aaacggcgaa gggcactttt gaaatgctta acttttgcaa
     3841 aaatatagct cttacgtaac tccgatttgt gcatcgagtg ggcgacaaac agccaatcaa
     3901 aagcactctg gtgattggca caaattatat atgctccggc cggaatattt tcgcgaccgc
     3961 taatttttat gcgcgaaaga aacctgaggg gcaaacagag aaggtttcgc aatgtaatat
     4021 gaggaaagcg gcttttgggt aattcaagag gatcttcatc tccggtttca ctgacaccgc
     4081 tgtttttgtt aatgccgata taatccgcta tggcttgtat agtcgggtat ttggataata
     4141 tatcttcatc cgcgtccata ttaaaacggg tttgaataaa gcttataagc gatagtttat
     4201 caagagagtc cagccctaca tcgatttcga tatgatcact aaccttgacc ttgcgtccag
     4261 tttcctttag tatataatcc ataattattg tggactcttt cgagtttccg gtttgatgat
     4321 agctttgttt gtcggttggt gccggggttc gctcaagttt aaagcgttga atcttgccca
     4381 aacgtgtgcg gggaaaatcg gtagaggtta gaatgacctt ttttatgcgt ttgtacgaag
     4441 aggcagaacg gttatacgga tcgataactt cctgcttaat atgttcttct atattcagta
     4501 tgccctgctt ttgtaaatat tcgctgtcag ggtgaataac agccgcaaga aggtcgtttt
     4561 cttctataac agctgcttct ttaacaagat cactgttctt ctccagtttt tgctcaatct
     4621 ctacaggatt aacgtttttc ccattcgaga ggatgatgat ctccttgatt cggccattaa
     4681 catgcagata accctttctg tcaaaataac ctttgtcacc cgtatgaagc cagccgtcat
     4741 agattacttc ggcagtttct tccgggcggt tataataacc tttcataatg ttacgaccac
     4801 ggcagcatat ttcatcgtta atgattttga cttccgtgtt tgttatcggg tatccaacat
     4861 tgcctatttt cactttgccg gggcggggac acgagatcat cggtgcagtc tcggtc
//
LOCUS       DAOTGZ010000205         4877 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_3064
            NODE_20655_length_4877_cov_4.353380, whole genome shotgun sequence.
ACCESSION   DAOTGZ010000205 DAOTGZ010000000
VERSION     DAOTGZ010000205.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868088
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 4877)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4877)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:22:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,785
            CDSs (total)                      :: 2,751
            Genes (coding)                    :: 2,732
            CDSs (with protein)               :: 2,732
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 12 of 19
            Pseudo Genes (internal stop)      :: 5 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4877
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_20655_length_4877_cov_4.353380"
                     /isolate="AMR_MDS_3064"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..405)
                     /locus_tag="P5123_11365"
     CDS             complement(<1..405)
                     /locus_tag="P5123_11365"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRX16902.1"
                     /translation="MKKNILQIALFYLIMMPCLITAKTTVTTESNPLFPDIELGGGEE
                     TRFKDDFADTVMANAPNFAEAFYLQNMLGYVVGKPHIKGWQAGFVLGSAFANGEYWDR
                     TAKPDTYPAFAPIVNFHLGKELDSRSDVIGKIM"
     gene            complement(479..1243)
                     /gene="folE2"
                     /locus_tag="P5123_11370"
     CDS             complement(479..1243)
                     /gene="folE2"
                     /locus_tag="P5123_11370"
                     /EC_number="3.5.4.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020250983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003933 - GTP cyclohydrolase activity
                     [Evidence IEA];
                     GO_process: GO:0035998 - 7,8-dihydroneopterin
                     3'-triphosphate biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP cyclohydrolase FolE2"
                     /protein_id="HRX16903.1"
                     /translation="MKDIQSQPDDRNMPIDQVGVKDIRFPVRVQDKRNGSQHTVATVN
                     MYVTLPHNFRGTHMSRFLEILNCYEEITPSHIPAILEEMKSKLNAETAYFDIEFPYFI
                     MKSAPVSKAKSVMEYICSIHGDSSAEFYFLKVKVPVMSLCPCSKEISRFGAHNQRSII
                     EISIKTRKKIWFEELITIAESSASSEIYSLLKREDEKYVTENSYNNPAFVEDIVRAIA
                     VKLKDHELIDGFSVESENMESIHNHSAYAKIVYNIL"
     gene            complement(1316..2065)
                     /gene="map"
                     /locus_tag="P5123_11375"
     CDS             complement(1316..2065)
                     /gene="map"
                     /locus_tag="P5123_11375"
                     /EC_number="3.4.11.18"
                     /inference="COORDINATES: protein motif:HMM:TIGR00500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I methionyl aminopeptidase"
                     /protein_id="HRX16904.1"
                     /translation="MRSGIIKSSDDIKRIAEAGAVLRALFEVLYTLDLEGLSTWKVDS
                     VVENFINSHGGRPSFLTLPGYRFASCISVNDEVVHGIPSKKKIIKSGDIVSIDAGVVK
                     NGYFADACRTFTIGSVNEELHYLVEANRSALKKAVSVMEDGAPLNAIGNEISAFAIKS
                     SLVILDSFTGHGVGYALHEEPVVFHGAGSCNDLKLRSGMVLAVEPVMAFKNCKYQKLD
                     DGWTVKTVDGSPTSQFEDTIAITENGPVVLT"
     gene            complement(2446..3561)
                     /gene="recA"
                     /locus_tag="P5123_11380"
     CDS             complement(2446..3561)
                     /gene="recA"
                     /locus_tag="P5123_11380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016545015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008094 - ATP-dependent activity, acting
                     on DNA [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0009432 - SOS response [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase RecA"
                     /protein_id="HRX16905.1"
                     /translation="MAQQKETQKEMNQKEQALSSAISQIEKQFGKGSIMKLGDEANRV
                     PIGSISTGSLELDVALGIGGIPRGRVTEIFGPESSGKTTLTLHIIAEAQKAGGIAAFV
                     DAEHALDPIYAAKLGVNTDDLLVSQPDTGEEALEITESLVRSGAVDIVVVDSVAALVP
                     RAEIEGDMGDSHMGLQARLMSQALRKLTGIISKSKTSVIFINQIRHKIGVMFGSPETT
                     TGGNALKFYSSVRMDIRRIETLKKGEDAYGNRVRVKVVKNKVAPPFRNAEFDIIYDKG
                     ISRQGGILDLGVKFDIINKAGTWYSYGEDRIGQGRENVKTYLEDNPNIAQEIELKVKK
                     ASGLIPDDEKPVKEEKTKEEKAIAEIEVSAEVATENE"
     gene            complement(3712..>4877)
                     /locus_tag="P5123_11385"
     CDS             complement(3712..>4877)
                     /locus_tag="P5123_11385"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRX16906.1"
                     /translation="REKNHQPPIIHIDKNEQYKYLLTSRGSDHEPILSSYPAYAGRVI
                     RFRSARKLVADIVNFKKKYGIIYFYIRDDNFLKDKDYVTTFCNELLRRKVYILWTCYT
                     DVYSLDEKLLVLMKKAGLHRIRLDLVSGSQRILNLYAPGVKIDQIKKAADSIRKVGIY
                     LSVRSFTGLPEEAEADMERTLAFLKRILPFKCNVVRAHYIPGTTLYDEAVMNGKLSPS
                     IWFKSSNPLIFCNEDPVVADWKHEISFGVEILRQQSWFNSKNFVTHRRVNGKDSWVTD
                     FIEGDYYLNTNQTAQADRVFRRIITVHPENCWGYLRMGKVKFRSGHFESAFDYYRTVT
                     DLVPEYYGGWLKAAQSLVADGRFKEAKKWIEEAYRRNRYDLRIHNVRDVLRHH"
BASE COUNT         1460 a         1100 c          784 g         1533 t
ORIGIN      
        1 cataatcttt ccaataacat cactacgtga atccaattcc ttacctaaat gaaaatttac
       61 aataggagca aatgccggat aagtatcggg tttggctgtt ctatcccagt attccccgtt
      121 tgcaaacgca cttcctaaaa cgaatccagc ctgccatccc ttgatgtgtg gcttacccac
      181 aacatatcct aacatatttt gcaaataaaa agcttccgcg aaatttgggg cattagccat
      241 tacagtgtcc gcgaagtcat ccttaaatct agtctcttca ccaccaccaa gctcaatatc
      301 cgggaataga gggttacttt ccgttgtaac tgttgtcttc gcagtaatca gacaaggcat
      361 cataatcaaa taaaacagag ctatttgcag aatatttttt ttcatcggat actcctcact
      421 ctattaacgg aaaacaaatg acctttcttt acacgaagtt atggtaatca ttgagatttt
      481 acaaaatatt atatactatt tttgcgtatg ccgagtgatt atgaatcgat tccatatttt
      541 cagactcaac agagaagcca tcaataagtt catgatcctt aagttttaca gcgattgctc
      601 gtacaatgtc ctctacaaat gcaggattat tataagaatt ttctgttaca tatttttcat
      661 cttcacgttt aagaagagaa tatatttcag aagatgcaga gctctctgct attgtgatca
      721 gttcttcgaa ccatattttt ttgcgtgttt ttatcgaaat ctcgattatc gaacgctgat
      781 tatgcgcgcc aaaacgactt atctcctttg agcatggaca aagggacata acaggcacct
      841 tgaccttaag aaaatagaac tccgctgaag agtctccgtg aatagaacag atatactcca
      901 ttacagattt agctttagat acaggagcac tcttcattat gaaataagga aattctatat
      961 caaaatatgc ggtctccgca tttagctttg atttcatctc ttcaagaata gcaggaatat
     1021 gcgagggcgt tatctcttcg taacaattta aaatttccag aaaacgactc atatgtgttc
     1081 ccctgaaatt atggggcaga gtgacgtaca tattaactgt agcaacagtg tgctgagagc
     1141 cattgcgttt atcttgaact cgaaccggaa aacgtatatc tttaacacca acctgatcaa
     1201 taggcatatt tctatcatca ggctgtgatt gaatatcttt cataaatata tcctcgaaca
     1261 taatagataa gcgaaaagta gcgacaatgc tagattatcc ataaagccat atcattcagg
     1321 taagcacaac cggtccgttt tcagttattg cgattgtatc ttcaaactgt gatgttgggc
     1381 taccatcgac agttttaaca gtccatccat cgtcaagttt ttgatatttg cagtttttaa
     1441 aagccataac cggctcaacc gctaaaacca tgccagatct caatttcaaa tcattacagc
     1501 tacctgctcc atggaataca accggctctt catgaagagc ataacctact ccatgacctg
     1561 taaagctgtc aagtataacc agtgaacttt ttattgcgaa tgcactaatc tcgttaccaa
     1621 tagcatttaa aggtgctcca tcctccataa ccgatacagc tttttttaaa gcggagcggt
     1681 ttgcttctac caaatagtgc agttcttcat ttacagatcc gatagtaaat gttcggcatg
     1741 cgtcggcaaa atatccgttt ttaaccacgc ccgcatcaat agagacaata tcacccgatt
     1801 tgattatttt tttcttactg ggaattccat gcacgacctc atcgttaacc gatatacagc
     1861 tggcaaaacg gtaccccgga agtgttaaaa aagaaggtct tccaccgtga gaattgataa
     1921 agttctcaac taccgaatcg accttccatg ttgaaagacc ctccaggtcg agagtgtaca
     1981 aaacttcaaa aagcgctcgc agcactgctc cggcctctgc aatccttttg atatcatcac
     2041 tgcttttaat tattccggat ctcatcaatc ttacggtttc atgcgaaata aaaccgtatc
     2101 tcccacacta gtttagcttt agttcaattc ttgacttgtc ttctgaatag tagatatata
     2161 atggtttacc aattatgtta atcaaaacac caattattcg attgacttat caattcatca
     2221 actgttattt attttttcgc taagttttaa ttcagatcaa tcttgataaa atccgaaatc
     2281 ctttcaacca tatttactct aaatctagac tataatattt ttttataatt gatacaccag
     2341 aaactttaac tctttactac gtgaaactta cttacacaaa agcccttcgg attttacctg
     2401 aagggctttt ttttatacag acttattatt tgcaaacata aaggtttact cgttttctgt
     2461 ggcaacctct gcactaacct caatctccgc tatagccttc tcttctttag tcttttcctc
     2521 tttaacaggt ttttcatcat caggtatcaa tcctgatgct ttttttacct ttaattctat
     2581 ctcttgggct atatttggat tatcttccag atatgtttta acattttccc ttccctggcc
     2641 aatacgatct tccccgtacg agtaccatgt tccagcttta ttaataatat caaacttgac
     2701 tccaagatca agaatgcctc cttgacgtga aattccttta tcataaataa tatcaaactc
     2761 ggcatttcta aacggtggcg ccaccttgtt ttttacaacc tttacacgaa ccctgttgcc
     2821 ataggcgtct tcaccttttt ttagagtctc gatgcgtctg atatccatcc ttaccgatga
     2881 ataaaactta agtgcattac caccggtagt tgtctcgggt gagccaaaca ttacaccaat
     2941 tttatgacgt atctggttaa taaatataac cgaagtttta gatttagaga taataccggt
     3001 cagcttcctt aacgcttggc tcataagtct ggcctgaagt cccatatgag agtctcccat
     3061 gtcaccttca atctcagcac ggggaaccag cgccgcaaca gaatctacaa ctactatatc
     3121 caccgcaccc gatcttacca ggctctcagt aatctctaaa gcctcttcac cggtatcggg
     3181 ttgagatact agtaggtcat cagtattaac accgagtttt gcggcgtaaa taggatcaag
     3241 agcgtgttct gcatccacaa atgcagcaat tcctcccgct ttttgagcct ccgcgataat
     3301 atgtaatgtc aatgttgttt tccctgatga ttcaggtcca aatatctctg taacacgccc
     3361 gcgtggaata ccacctatac caagtgcaac gtcaagttca agcgaacctg tactgattga
     3421 acctatcggt accctattgg cctcatcccc aagcttcata atggaacctt ttccaaactg
     3481 cttctctatc tgggatatcg ccgacgataa agcctgctcc ttctgattca tctctttttg
     3541 agtctctttt tgttgtgcca ttttcccccc ttagaattat acttttttgc acgaccaaaa
     3601 ccgtgcaggc atgtgcctgc tattattaac tgattttttc aaaaaagtgt cgaacttttt
     3661 tgtcatattg aaatattatc ggaaagaagt caagaactac ttaaattatg tttagtggtg
     3721 acgtagaaca tcccttacat tatgtattct aaggtcatat cgattacgac gataagcttc
     3781 ctctatccac ttttttgctt ctttaaagcg tccatcggcc acaagcgact gtgcagcctt
     3841 aagccatccc ccataatatt cgggaacaag atcagtaact gtacggtaat agtcaaaagc
     3901 actttcaaaa tggccacttc taaatttaac tttgcccatt ctaagatacc cccagcaatt
     3961 ctctggatgt acggtgataa ttcttcgaaa gacccgatca gcctgagcag tctgattcgt
     4021 attaagataa taatcaccct caataaaatc ggttacccaa gaatcttttc cattaacacg
     4081 tctatgagta acaaagtttt tactattgaa ccaagattgc tgacgcaaaa tttcgacacc
     4141 aaaagatatc tcatgtttcc aatctgccac gacaggatct tcattgcaaa atataagtgg
     4201 attagaactc ttgaaccaga ttgacggtga cagtttgcca ttcatgactg cttcatcata
     4261 taacgttgtt ccaggaatat aatgtgccct aacaacatta catttaaaag gcaaaattcg
     4321 tttaagaaac gcaagagttc gctccatatc cgcctcagcc tcttcaggaa gacctgtaaa
     4381 tgagcgaaca gacagataaa ttccaacctt gcgaatacta tcagcagcct ttttaatttg
     4441 atcaatcttt acaccaggag cgtaaagatt caaaatcctc tgtgaaccgc taacaagatc
     4501 aagtcttata cgatgaagtc ccgccttttt cattaataca agaagtttct catcaagact
     4561 gtatacatcg gtataacagg tccataagat atagaccttt cttcttaaca gctcattaca
     4621 aaatgttgta acgtaatcct tatcttttaa aaaattgtca tcgcgaatat aaaaatatat
     4681 aataccatac ttttttttaa agttaacaat atcggcgaca agtttgcggg ctgacctaaa
     4741 acggattacc cttcctgcat aggccggata agatgataaa ataggttcat gatccgaacc
     4801 acgactggta agcaaatatt tatattgctc atttttatcg atatgaataa tgggcggctg
     4861 atggttcttt tcccgga
//
LOCUS       DAOTGZ010000206         4873 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_3064
            NODE_20671_length_4873_cov_5.820257, whole genome shotgun sequence.
ACCESSION   DAOTGZ010000206 DAOTGZ010000000
VERSION     DAOTGZ010000206.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868088
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 4873)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4873)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:22:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,785
            CDSs (total)                      :: 2,751
            Genes (coding)                    :: 2,732
            CDSs (with protein)               :: 2,732
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 12 of 19
            Pseudo Genes (internal stop)      :: 5 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4873
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_20671_length_4873_cov_5.820257"
                     /isolate="AMR_MDS_3064"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(255..731)
                     /locus_tag="P5123_11390"
     CDS             complement(255..731)
                     /locus_tag="P5123_11390"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRX16907.1"
                     /translation="MESTDFRTSIDVEVTRMQRIEEYADKHGMDVSDVMNTVLDKYAE
                     KLAKNPVCNNTLNRYQERGTIYKSVHIRFSIISKRKLKDIANIYCYSLSYMLYLAMDF
                     FEEFMNDVKEFSRPCFHAVFVRLFEFPCFINSWGMNFYDSADHPGQQTRNLFHLLN"
     gene            complement(1344..2039)
                     /locus_tag="P5123_11395"
     CDS             complement(1344..2039)
                     /locus_tag="P5123_11395"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0070064 - proline-rich region binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SH3 domain-containing protein"
                     /protein_id="HRX16908.1"
                     /translation="MSKILQFIFIIIIINITAFSEGYVDINQSGYVNGHMVNVRLNES
                     TSSTSKGYLLHGTVLDVKYRTAKKDTISGSSNYWYFCDHCDGCYGWIFGDFVKLGKNP
                     DDIFTKYVIPNFPDNFTIISEYSWSDIAPQDRNKGERGGRDEFFTIRNNILILDTDYL
                     LVPKYLIEEVNEVSSNHFVLKIRLIAIDSRVNKKETQLFDIHMIYNPKTEILSLNSKS
                     IKQDKLYRCKKLK"
     gene            complement(2290..3093)
                     /locus_tag="P5123_11400"
     CDS             complement(2290..3093)
                     /locus_tag="P5123_11400"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HRX16909.1"
                     /translation="MILTAIKIIISVTVILALIYVSERNAKLGGIFAGLPIGTGIMVF
                     FYAIEQGNNFMLASIPYAISGLSSTLAFTAGFYLGGKFFVNRPVIKILSSLICSMICY
                     FLVSYMISLININLLKSLIIFSLSTLIANLFFSHIPAQKKIAVKKLTVCSISFRIIFI
                     TMSILVITGLANTIGSKWAGIMASFPSALCPLLLVLAFLYDDQLYPSVIKSFSYSITT
                     ISIFYLFILWLVPITGLFIATALTYLICFFYIYLLNLVLNNRIFLRESK"
     gene            3345..4532
                     /locus_tag="P5123_11405"
     CDS             3345..4532
                     /locus_tag="P5123_11405"
                     /inference="COORDINATES: protein motif:HMM:NF014825.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SEC-C metal-binding domain-containing protein"
                     /protein_id="HRX16910.1"
                     /translation="MSHKVGRNDPCPCGSGKKYKKCCMEKADNVLRFPSGTQSGSVPN
                     NLIPPLPGFDPEKFAEYQTFAESWDEADGPVPSFMQYLGTGNAASEVLDDLKNNLKGM
                     DIKSEKQLQALADMHMRSHNNTPIDELLGLNPVQMRYLLDTDFELGSGAIKLSDKVDP
                     DLYVNAPFILWTLWLFSYIEGAGEIQLSEYELLRDEVIDKFIDNILLRGKPHDEVMSQ
                     IKDNKNKNDLIPALREQAFALNVCFLFLSNNGYVKKRKGMYSLTAKIKKMIKAKGFID
                     KLFRECFMWMCYSYNWLGFYDYDIEDIQATLPFSLHIVHRKCQDFVQCDKVADYFFGA
                     FGHIIKDNELSRMAYMVLFLDNFAELFGLIDIKYTKSKSGLLRNISIRESELAKQLFV
                     WNV"
     gene            4700..4873
                     /locus_tag="P5123_11410"
                     /pseudo
     CDS             4700..4873
                     /locus_tag="P5123_11410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006966960.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4143 domain-containing protein"
BASE COUNT         1502 a          784 c          953 g         1634 t
ORIGIN      
        1 agcccgaaaa tgttccatgc atatcaccac tttcgcggag tgccagcgac gcgaagtggt
       61 gaagcagaac aattgcgcaa tcttcaagcg cgattgtttg gacggtttcg ggcgaatagc
      121 ccgaaaatgt tccatgcata tcaccacttt cgcggagtgc cagcgacgcg aagtggtgaa
      181 gcagaacaat tgcgcaatct tcaagcgcga ttgttttgac ggtttctttt atctattccg
      241 ttaaaaaatg ttgatcagtt taataaatga aacaaatttc tggtttgttg tccaggatgg
      301 tcagctgaat cgtagaaatt cataccccag ctgttaatga agcatggaaa ttcgaacaag
      361 cgaacaaaaa ctgcatgaaa acagggtctt gaaaactcct taacatcatt cataaactct
      421 tcaaaaaaat ccattgctag atacaacatg taagacagac tataacaata aatattggct
      481 atatctttta gttttcgctt cgaaataatt gaaaatctta tgtgtacaga tttataaatt
      541 gtaccccgtt cttgatatct atttagagta ttattgcata caggattctt agccagcttt
      601 tctgcatact tatcaagaac tgtgttcatt acatccgaaa cgtccatgcc gtgtttatcc
      661 gcatactctt cgattctttg cattctggta acttctacat ctattgatgt tcgaaaatcg
      721 gtagattcca ttttgttcct ccaaagattg atctagatat gaatactgga ttggaggcgg
      781 ttttgttaaa attttttctc attttctttc ttttctggag ggagagtatg cttttgatgc
      841 aagttggaga aggcggcatc attaatactg ttttaactaa ggctacaaat gcgagtatct
      901 aaaaaggaaa atctaatcgt tgagttatct gcattgaaaa tatagaggac gaaataaatt
      961 acatgtaact atggactgta tagtaatatg ttttggctat ggatatcggt ccgataatag
     1021 tgattgtttt gtctatccat gcagtagtgg gctttccgta cattttagcc caggacttct
     1081 tttccgaaat gttatataaa tttgatcccc tttcggaata actgcccaga ggtcatggtt
     1141 ctattttttg taattgtcga tgttctatct gatccagtct gtaactattt cttcgctaaa
     1201 cggttgggga taatacttta ttgattcggg ataggttagt attacagata attggccttt
     1261 ttcttttgtt gctagttatc agatatagag tctttaggtt tcaataattt ttttataata
     1321 atgtctttta aaatgagcgt tgattatttt aatttcttac atctgtacag tttatcctgt
     1381 tttatacttt tactgtttaa actcaggatt tcagtttttg gattataaat catatgaata
     1441 tcaaaaagtt gagtttcttt tttgttaacc cgactatcta ttgcaatgag tctgattttt
     1501 aatacaaagt ggttagagga tacttcatta acttcttcga ttagatattt tggcaccaac
     1561 aggtagtctg tatctaatat caaaatattg tttcttatag taaaaaactc atctctgccc
     1621 cctctttctc ctttgttacg gtcttgaggt gctatatcgc tccagctgta ttctgagata
     1681 atggtgaaat tatctggaaa atttgggatt acatattttg taaaaatatc atccggattt
     1741 ttccctaatt taacaaaatc accaaatatc caaccataac agccatcaca atgatcgcaa
     1801 aaataccaat aatttgagga tccggaaatc gtatcttttt ttgcggtgcg gtattttaca
     1861 tctaaaaccg tgccatgtaa gagataccct ttagaagttg aactggttga ttcgtttaaa
     1921 cgaacattaa ccatatgtcc attaacatag ccggattgat ttatatcgac atatccttca
     1981 gaaaatgctg tgatattaat aataattata atgaagatga actgtaagat tttgctcatg
     2041 ttgcctctat taaataatct tcagaagagt tgttgtttca gcaagcattt atttgaagac
     2101 ttcttgtcgt acagattgtt aaatatagca atctattttt agttttgaaa tgattaaacc
     2161 ggtctatatt aaatacagtc atcaacttct tttcggagtc aattattatt gcttaagatt
     2221 agtttttcta ttttggttta tacgaattat ataaataatt attttctaaa gtattcaatt
     2281 gcacgtatat tatttgcttt ctcttaaaaa tatgcggttg tttagcacaa ggttaagtag
     2341 gtaaatataa aagaaacaga ttaggtatgt caatgccgtt gcaataaaaa gaccggttat
     2401 gggtacaagc cacaaaataa ataaataaaa aattgatatt gtagtgatgc tgtacgaaaa
     2461 actttttatg acagaggggt ataactggtc atcatataaa aaggccaaaa ccaaaagtag
     2521 aggacagagc gcggagggga aagatgccat aatgcccgcc cactttgaac ctattgtgtt
     2581 tgcgagtcct gttatgacga gtatggacat ggttataaaa attattctga atgatatgga
     2641 acagacagtc agttttttga cggcaatttt tttttgtgcc ggaatatgag agaagaatag
     2701 attggcgata agagttgata gcgaaaaaat aataagactc tttagtaggt taatatttat
     2761 cagagagatc atgtacgaga ctaaaaaata acagatcatg ctacagatta aagatgataa
     2821 tatttttatt acaggtcgat taacaaaaaa tttaccgccg aggtaaaatc cggcagtaaa
     2881 tgccagtgtg ctgcttaatc cggatattgc gtagggaata cttgccagca taaaattgtt
     2941 tccctgttcg attgcgtaaa agaataccat aatgccggtg cctatcggta atccggcaaa
     3001 aatacctccc agtttcgcat ttctttcaga aacatatatt agtgcgagaa ttactgtaac
     3061 cgagatgatg atttttattg ccgtcaatat catggatgtt accctagagt attataaaat
     3121 tttttattaa taattgcatc ataggtgatt agctctttat ttgcgtcaag caattctgtc
     3181 ataaaccatc acgtgtggtt actgcgtttt gcaccatctc taaaggctga tgggtataaa
     3241 tcttgctgca gcttggtaaa gtttagatat aactgagtaa aaaatagttg tataaaccag
     3301 tcattcgttg tacaattttt aaaattataa ccaaggaaca gttaatgagc cataaagttg
     3361 gaagaaacga tccctgccca tgcggtagtg ggaaaaagta taaaaagtgt tgtatggaaa
     3421 aagcggataa tgtgttgcgg tttccgagtg gtactcaatc aggtagtgtt cccaataatt
     3481 tgattccacc tttgcccggt tttgatccag aaaaatttgc tgaataccag acattcgctg
     3541 agtcatggga tgaagctgat gggcctgttc ccagctttat gcagtattta ggtacgggta
     3601 atgccgcaag cgaggtgctt gatgatttaa aaaataacct taaagggatg gacattaagt
     3661 cagaaaaaca gctgcaagca ttggccgata tgcatatgcg gtcacacaac aatacaccga
     3721 ttgatgaact gttaggtctt aaccctgtgc aaatgagata ccttttggat accgattttg
     3781 aactaggatc aggcgccatt aaattgtctg ataaagttga tcccgatctg tacgtaaatg
     3841 ccccttttat tttatggacg ttgtggcttt tcagctatat tgagggtgcc ggcgagatac
     3901 agctctctga atatgaactg ctacgtgatg aagtaattga taaatttatt gataatatct
     3961 tacttcgggg taagcctcat gatgaggtta tgagtcagat aaaggacaac aagaataaga
     4021 atgacctgat accggcattg agagaacaag ctttcgcatt aaatgtttgt tttttatttt
     4081 tatctaataa tggttatgtc aagaaaagaa aaggcatgta ctcgcttacc gcgaaaatca
     4141 agaaaatgat aaaagccaaa ggatttattg ataagctgtt tcgtgaatgt tttatgtgga
     4201 tgtgttatag ttacaattgg ttagggtttt atgattatga tatagaagat attcaggcaa
     4261 cgttaccttt tagtttgcat attgtacatc gtaaatgtca ggattttgta cagtgtgaca
     4321 aagtggcgga ttattttttt ggggcgtttg gacatattat caaagataat gagttatcgc
     4381 gtatggcata tatggttctg tttttagata attttgcgga gttatttggt cttattgata
     4441 taaagtatac aaagtctaaa tcaggtttgt taagaaacat aagcattcgt gaatctgagc
     4501 ttgctaagca gcttttcgtc tggaatgttt agcgttgctg gtctttgtgg attgttagcc
     4561 acctttttcg agtgttcgaa gcaaaaatct ggattgttag ccactttgtt aaggttttgg
     4621 gagtaaaacc tggattgtta gccaccctca gagataatag gggtgcaaat tgcacttgac
     4681 aagttcaatt tgcacccctg aatcacccaa actctatttt tatgatgtag ggctcgccgc
     4741 taatctgctt ggtatcgaaa acaagggaca ggtcgctact catccactgc gcggtgcgct
     4801 ttttgaaaac cttgtaataa cagaaatact caagactagt tttaataatg ttaaaacaaa
     4861 caacctcttt ctt
//
LOCUS       DAOTGZ010000207         4870 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_3064
            NODE_20692_length_4870_cov_5.749117, whole genome shotgun sequence.
ACCESSION   DAOTGZ010000207 DAOTGZ010000000
VERSION     DAOTGZ010000207.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868088
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 4870)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4870)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:22:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,785
            CDSs (total)                      :: 2,751
            Genes (coding)                    :: 2,732
            CDSs (with protein)               :: 2,732
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 12 of 19
            Pseudo Genes (internal stop)      :: 5 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4870
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_20692_length_4870_cov_5.749117"
                     /isolate="AMR_MDS_3064"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..378)
                     /locus_tag="P5123_11415"
     CDS             complement(<1..378)
                     /locus_tag="P5123_11415"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HRX16911.1"
                     /translation="MIKVLKEKSDEHVVRSVLNGNSADFEIIVNRYKDRLFSIGYRFF
                     YNRDDAEDFVQDLFVRVFERLDSFRFDAPFRYWLVRIAYNYGINQKKSKVIELDVSDY
                     EMPDSKSSPENKPIRDEIIALLNE"
     gene            complement(350..718)
                     /locus_tag="P5123_11420"
     CDS             complement(350..718)
                     /locus_tag="P5123_11420"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRX16912.1"
                     /translation="MKVDSATVAVQVMITVIPIVGILMGGAVIILFLILNHKQKMAMI
                     RHNYGSKQPIDIVWISLFAGLLLTFIGFVMSLFFIIKEGVEYSLLSGIVPLAAGMGLM
                     IFFTIARKSGQIDKSSERKE"
     gene            complement(865..2382)
                     /locus_tag="P5123_11425"
     CDS             complement(865..2382)
                     /locus_tag="P5123_11425"
                     /inference="COORDINATES: protein motif:HMM:NF013646.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent monooxygenase"
                     /protein_id="HRX16913.1"
                     /translation="MIKRIQIQMPPDYNQDNLSKVVAEISNIKKPFFSIVSRSLDARK
                     AGNIHYCLNIDVSPNPLPAKDSLKIPLVKKNISAIVVGIGPAGYFAALVLSRAGISVT
                     VIEKGKKVCERENDILHFENSGSLVENSNYLFGEGGAGTFSDGKLTSRSKRISLEKEF
                     ILKTYIEAGADAEIEFDAHPHLGSDRLREMMPELRKILLRQNVQILFETEFKSFIKKN
                     NKFLVEASNCKLESDLLFIAPGHSSYNTYRSLIRSNVKFRTKPFAVGFRVEHPQILIN
                     RAQWKRDSLPGIRSAEYRLSYINGDVRVYSFCMCPGGYIVPAAVSQGHSAVNGASNYL
                     RNGKYSNAAIVSAFTLESRLKKTVLPLEVLDYVEEQTSMLFNCTKSYNMPAVTISDFL
                     KNRVSSVLPECSYPFEIFPYDYNIIFDNRLCSDLREGLKDFCNKLKGFEQGLIVGSEY
                     TTSAPIQCFRDKEGEVEGFEKLYICGEGSGFAGGIISSAADGVRLAMNAVCKISD"
     gene            complement(2379..3665)
                     /locus_tag="P5123_11430"
     CDS             complement(2379..3665)
                     /locus_tag="P5123_11430"
                     /inference="COORDINATES: protein motif:HMM:NF018884.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016791 - phosphatase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SpoIIE family protein phosphatase"
                     /protein_id="HRX16914.1"
                     /translation="MNTLLPPRNIRTIYYTHMMSILYIFLLVVLAIIEFHINYTTLFF
                     FLLSLMFLLVIFNSILFTRHYSVRFYRCPLYAIAAISSVICAVLSKNAVAIAVISAPL
                     PVILISEKSVLRQIIWLAAVPIVWSSLLYIHLCGKYSVFNTDYNDIVIITVFYVLSAT
                     ASVLYRFSIGRTFWGNLSKYRRTDIIDIQMELAQVIQRTLIPEKSKTGDKYQAAFYYH
                     PAYGIGGDYFDIFEMSDGRVGVVMCDVAGKGIPAALMMASVRTLTRALVSQGINDPLE
                     IIRILNDTFRLDFGDEFYVTLAFLCFDSKTGMIDFYNAGHTPFIYYSRVEKRIMEIEL
                     DGYPIGVFDQLYHTGKITEKMYSGDIVVLFSDGLTDFIDSGNHASARSRLFLEIQKNA
                     DLDAQTITDRIIDFFIKDKKSNIVDDDISLVIFKVK"
     gene            complement(3752..4051)
                     /locus_tag="P5123_11435"
     CDS             complement(3752..4051)
                     /locus_tag="P5123_11435"
                     /inference="COORDINATES: protein motif:HMM:NF013531.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Trp family transcriptional regulator"
                     /protein_id="HRX16915.1"
                     /translation="MADIKNIAAVLADCTKSSDMKQLLEELFTPAELSNLELRWELLK
                     RLASGDSQRTISSDLGISLCKITRGSKLIQDNSSQVYKILVGSDKKAKNRTKSSK"
     gene            4271..>4870
                     /locus_tag="P5123_11440"
     CDS             4271..>4870
                     /locus_tag="P5123_11440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012176193.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HRX16916.1"
                     /translation="MITVNENFLKLKASYLFSDIAKKVKEFQNNYPDRELIRLGIGDV
                     TEPLPSACIEAFHKAVDEMSDTNTFHGYGPEQGYAFLREAVSENEYKQHGADISPDEI
                     FISDGSKCDTGNFQELFSNDINIAIPDPVYPVYVDTNVMAGRTGLNNNGRYDNLIYLD
                     SNENNGFVPDPPSTKADLIYLCFPNNPTGAIATKEQLKRW"
BASE COUNT         1625 a          968 c          777 g         1500 t
ORIGIN      
        1 ttcgtttaac agggcaatga tctcgtctct tataggttta ttttcaggag atgatttgct
       61 atccggcatt tcataatctg atacatcaag ctcgataacc tttgattttt tttggttgat
      121 accgtaatta taagcaatac gaaccaacca gtagcgaaag ggcgcatcaa accggaaaga
      181 atctagcctt tcaaagactc ttacaaaaag atcctgtaca aagtcttcag cgtcatctct
      241 gttataaaaa aatctgtaac caatggaaaa taatctatct ttataacggt taacgattat
      301 ttcaaaatct gctgaattac cgtttaaaac cgaacgaaca acatgttcgt cactcttttc
      361 tttcagaact tttatcaatt tgacctgatt tacgtgcaat agtgaaaaaa atcattagcc
      421 ccataccggc cgctagcgga acaataccgc ttaacaatga gtactcaaca ccctctttaa
      481 taataaaaaa aagtgacatc acaaaaccaa taaaagttaa tagtagccct gcaaaaaggc
      541 tgatccaaac aatatctatt ggctgttttg atccgtaatt atgccttatc atagccatct
      601 tttgcttatg attcagtatc aaaaaaagta ttattacggc tccccccatt aaaataccta
      661 caataggtat tacggtaatc ataacctgaa cggcaacagt agcactatcc actttcataa
      721 cctcaatctt ttcacattat tattatattg actccatttt atcaaacggg ttgcatattt
      781 tttaagataa cagagcaaag taagaaaaat aaaaaatata aataatagtc aaaatttatc
      841 tgtcaaatta aatataaatt atacttaatc ggatattttg catacagcat tcatagccaa
      901 cctgacacca tcagcagcgg atgagataat tccaccagca aatccgctac cctcaccgca
      961 tatatacaat ttttcaaacc cctctacttc cccctcttta tcacgaaaac attgtattgg
     1021 tgctgaagtt gtatactcac ttccaacaat aaggccctgt tcgaaacctt ttagtttatt
     1081 acaaaagtct tttaaacctt ctctaagatc agaacatagt ctgttgtcaa aaataatatt
     1141 ataatcgtat ggaaatatct caaatggata cgagcactcc ggtaaaacgg aagagactct
     1201 gtttttcaaa aaatcagata tcgtgaccgc aggcatatta tagctttttg tacagttaaa
     1261 caacatcgat gtctgttctt caacataatc gagaacctca agaggtaata ctgttttttt
     1321 taatctgctc tctagagtaa aggcagaaac aatcgccgca ttagagtatt taccgttacg
     1381 taaataattt gacgctccat ttaccgccga atgtccctga ctaaccgcag caggaacaat
     1441 atatccacct ggacacatac aaaaactgta cactcttaca tcgccgttta tatatgacaa
     1501 cctatattca gcggaacgga ttccaggaag tgaatcacgt ttccattgag ccctgttaat
     1561 caatatctgg ggatgttcca ccctaaaacc tactgcaaat ggtttagttc taaatttaac
     1621 attactacga attaatgatc tataagtatt gtaagaggag tggccgggag cgatgaagag
     1681 caggtctgat tctaatttgc aattggaagc ttccactaaa aatttgttat ttttttttat
     1741 aaaagatttg aattcggttt caaaaagaat ttgaacattt tgtctcagaa gaattttcct
     1801 tagctccggc atcatttccc ttaacctgtc actgcctaga tggggatggg catcaaactc
     1861 tatttctgca tcggctccag cttcaatata agtttttaat ataaattcct tttccagaga
     1921 aattcttttt gaacgcgatg ttaatttccc gtcactaaat gttcccgctc ccccttcccc
     1981 gaaaaggtaa ttcgagtttt ctactaaaga gccactattt tcaaaatgaa gaatatcgtt
     2041 ttctctttca caaacttttt tacctttttc tataacagtt acacttatac cggctcgaga
     2101 taatacaaga gcagcaaaat aaccagcagg accgatcccc acaacaattg ccgagatatt
     2161 ttttttaacc aaagggattt ttaaggaatc cttggcaggt aatggatttg ggcttacatc
     2221 gatatttaaa caatagtgaa tgttaccagc ctttcgtgcg tcaagactac gtgatacaat
     2281 cgaaaaaaaa ggttttttga tattgcttat ttctgcaaca actttcgata agttatcctg
     2341 gttataatcg ggaggcattt gtatctgaat acgttttatc atttaacttt aaatattaca
     2401 agtgagatgt catcatcaac aatattggat tttttatcct ttataaaaaa gtcaattatt
     2461 ctgtcggtta ttgtctgagc atcaagatct gcattttttt gtatttctaa aaacagcctg
     2521 cttcttgccg aagcatgatt tccactatcg atgaagtctg taagcccgtc agagaataat
     2581 acgacaatat caccagaata cattttttca gtaatttttc ccgtatgata aagctgatca
     2641 aatacaccga taggataacc atccaattcg atctccatta ttctcttctc gacacgggaa
     2701 taataaataa agggcgtgtg tccggcatta taaaaatcta tcattccagt tttagaatca
     2761 aaacatagga atgcaagcgt aacataaaat tcatcaccaa aatcaaggcg aaaagtgtca
     2821 ttcaatatgc gaattatttc taatggatca ttaataccct gagatactag cgcacgtgtt
     2881 aaggtgcgta cacttgccat catcaaagca gcaggaatac cctttccggc aacatcgcac
     2941 ataacaaccc ctacgcggcc gtcactcatc tcgaaaatgt caaaataatc accgccaata
     3001 ccataagctg ggtgataata gaaagcagct tgatacttat cgcctgtttt acttttttcg
     3061 ggtatcaatg ttctttgtat aacctgagca agctccatct gaatatcgat gatatcggtt
     3121 cttcgatatt tggaaaggtt accccaaaac gtacggccaa tactgaatct atataaaaca
     3181 gatgccgttg ctgacaatac atagaaaacc gtaataataa cgatatcgtt ataatctgta
     3241 ttaaacacag aatatttacc acaaagatga atgtacaaaa gtgaagacca aacaatagga
     3301 acagccgcca accagattat ttggcgtaaa actgattttt ctgatattag aatgaccgga
     3361 agaggcgcac ttattaccgc aatcgcaacc gcatttttac ttaagaccgc acaaatgact
     3421 gaagaaattg cagcaattgc gtataatgga catctgtaaa accttacaga atagtgtcta
     3481 gtaaagagta tactgttaaa aataaccagt aaaaacatta gcgaaagcag aaagaaaaaa
     3541 agcgtcgtat aatttatatg aaattcaata attgcaagta cgacaagaag aaaaatataa
     3601 agaatcgaca tcatatgcgt ataatagatg gttcttatgt ttctaggggg caagagggtg
     3661 ttcatccccc ttataccata taattttaaa aacagccggt ctctgaattt cactgtcata
     3721 gaatgttctt attaataatt ttgtaaacca attatttaga acttttagtc ctgtttttgg
     3781 cttttttatc ggatccgaca agtattttat aaacctgtga tgagttatct tgtattaatt
     3841 ttgatcctct tgttatcttg cacagcgata tgcctaaatc agaagatatt gtacgttgtg
     3901 aatctcctga agcaagccgt tttaatagtt cccatctcaa ttcaagatta gatagttcag
     3961 ccggagtgaa taactcttcc agcaattgtt tcatatcact gctttttgtg caatctgcca
     4021 aaacagcagc tatattttta atatccgcca taaatccgcc tttttacatt agttaagaaa
     4081 cagaataatt ttacataatt gtaatatatc agttttttga ctccttgaca ccagtacaaa
     4141 cagtaaagga caatattttg tatagaaagt ctctgaacct gtgaatatcc ggtttttaat
     4201 atcttcaaac aaaaaatatt tttgctattc agagattttc ttttaacttc ttagtatata
     4261 aggatactat atgattactg taaacgaaaa ttttcttaaa ctgaaggcat catatctttt
     4321 ttcggatatc gcgaaaaaag taaaagagtt ccagaataat tatcctgatc gtgaactgat
     4381 aaggcttgga attggtgatg taacagaacc cttgccatcc gcatgtattg aagcctttca
     4441 taaggccgtt gacgagatgt ccgacacaaa tacatttcac ggatacggcc ctgaacaagg
     4501 ttacgctttc ttgcgagagg ctgtttctga aaatgagtac aaacaacatg gagcagatat
     4561 tagccccgat gagatcttta taagcgacgg ttcaaaatgt gataccggca actttcaaga
     4621 acttttctca aatgatatta acatagccat tcccgatcca gtttaccctg tatacgttga
     4681 cactaatgta atggctggcc gtaccggact taataataac gggagatacg ataatcttat
     4741 ttatcttgac tcaaacgaaa acaatggatt tgtaccggac cccccctcga ctaaagcaga
     4801 tctgatttac ctctgtttcc ccaataatcc tacaggagca attgccacaa aagagcaact
     4861 taagagatgg
//
LOCUS       DAOTGZ010000208         4856 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_3064
            NODE_20764_length_4856_cov_4.173506, whole genome shotgun sequence.
ACCESSION   DAOTGZ010000208 DAOTGZ010000000
VERSION     DAOTGZ010000208.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868088
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 4856)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4856)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:22:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,785
            CDSs (total)                      :: 2,751
            Genes (coding)                    :: 2,732
            CDSs (with protein)               :: 2,732
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 12 of 19
            Pseudo Genes (internal stop)      :: 5 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4856
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_20764_length_4856_cov_4.173506"
                     /isolate="AMR_MDS_3064"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            77..1084
                     /gene="xylF"
                     /locus_tag="P5123_11445"
     CDS             77..1084
                     /gene="xylF"
                     /locus_tag="P5123_11445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019638606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009897 - external side of plasma
                     membrane [Evidence IEA];
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0015614 - ABC-type D-xylose transporter
                     activity [Evidence IEA];
                     GO_function: GO:0033222 - xylose binding [Evidence IEA];
                     GO_process: GO:0015753 - D-xylose transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-xylose ABC transporter substrate-binding
                     protein"
                     /protein_id="HRX16917.1"
                     /translation="MAVTIFACSSCAKEKVPGKIKIGLSMDSLKEERWQKDRDIFKAV
                     AELMGAEVVIQAAQGNDATQIAQCENLITQGVDVLVIIPHNGEAMAVAVEKAHESGIP
                     VIAYDRLIMNCDLDIYISFDNVRVGELQATEITKLVPKGNYVYIGGAPTDNNALLFKE
                     GAMNILNPLIEKGDINLVFDQFSDDWSASIAQKHMENALTKNNNEIDAVIAANDGTAG
                     GAVQALSEQGLAGKVPISGQDADIAGCMRIVEGTQSMTVYKSIKSIAAEAVKLSIRMA
                     KGESIAAANTVNNNKKDVPSVLLTPVAVTKENMYQVVVRDGHHRLEAVYKNIPKDQWP
                     E"
     gene            1126..2298
                     /gene="gguB"
                     /locus_tag="P5123_11450"
     CDS             1126..2298
                     /gene="gguB"
                     /locus_tag="P5123_11450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013781957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HRX16918.1"
                     /translation="MNKTVSTEIISADNKKSILNINIRDYTMIIALVFIWIVLTVLTD
                     GFFIGSRNLSNLMRSISVTSALSLGIFLIIVAGHIDFSTGSVCGFIGAIAAILNVWLG
                     WGTFTSIVVALFLGMVIGFAQGYLVAYQKVPAFIVTLGGLMIFRGLLVGVTRGITVAP
                     LSASFVAIGQNYVLPVIGWIIAGVAVSAIWVLELRTRSTRLKYGFSVSSFKIVVAKSI
                     LYSGIVLLFVAIMNNYRGIPLPVFIIMLIALSLAFVACRTQFGRYVFALGGNIDAARL
                     SGINVELTTMVLFVVGGFMSGVGGIILTSRLNAATTDAGNMYEFDAIAACIIGGTSIK
                     GGAGTVIGVIVGALVMASLDNGMSLLNTNTFWQHVVKGVILIAAVWFDINAKNKKI"
     gene            complement(2308..3375)
                     /locus_tag="P5123_11455"
     CDS             complement(2308..3375)
                     /locus_tag="P5123_11455"
                     /inference="COORDINATES: protein motif:HMM:NF024242.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HRX16919.1"
                     /translation="MILLKVKNLTKYVDNALCFNQSNLVVHSNEICCIVSDNDSRARL
                     FVSVLADQEKKDSGEVLFKGERVISDHIGNQIGIAWQSKSLFMNLSGFDNIALKGGKH
                     YSNFGIIHRKRLLSDIQSLLKRFDTDIDLGAPIKDYNLNERTIIDLISIILLKPKLSI
                     FYKVSRYLTLNQFETLEQIIEEKKKAGNGIIYIPENLEESQIVGDKFYFLTEDGFVEI
                     TDHKSRSIDELRNQATSSEELNILSIYDPIYNTKKIMCKSLSEESIDFNELAAKVGMH
                     YHVFRKMFKEKTGISPKQFFLRLKIDKAKELLLKTNKKIDEIAEIVGFSDPLYFSRMF
                     KSKEKISPQNFRKQKSKIYFD"
     gene            complement(3467..4651)
                     /locus_tag="P5123_11460"
     CDS             complement(3467..4651)
                     /locus_tag="P5123_11460"
                     /inference="COORDINATES: protein motif:HMM:NF019310.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HRX16920.1"
                     /translation="MKKRDMLLILAAYILQIYMGITIAIRGFLLPEINNDFSINYQSS
                     GLMFLVPLIPALIGSFFGGYLYKIMRRKIILLSGTLLLTLLQVALAYSPNYLFFLAIN
                     SLLGFATLIVILGVNIGVSAFFQAHAYRYRESSIHLLHFFFGAGAIIALAFAKTILDF
                     SGSWRYVYLSFSSVSLLTFIILIIATFPDSEHKNSQKPKEHKFRLPVITSKVMLFFAM
                     AAIGYVGIEFTIINWAPTFMEDFLGQSKDHISKVMIAFFILFTAGRLVSALISHRIKQ
                     EHLFALLSGITLLIVVPAFILPVSIYGCYVFIPLSGLTISGLFPIIQNGIIKRFNSEI
                     DLATGSLYASSNIGSAFMPFFAGAISHQFGLSRGLYLLPFLTLLMILSYLLAGRVAYN
                     PD"
BASE COUNT         1457 a          841 c         1055 g         1503 t
ORIGIN      
        1 tgccatgttt ttatcgtatt atcatgaaat ctggaggtta taggtgattt caaaatcgct
       61 tcgtgttttt gtaataatag ctgtaacgat ttttgcttgt tcgtcttgtg ccaaagaaaa
      121 ggtgcccggc aagattaaaa taggtctttc aatggactca ttaaaagaag agagatggca
      181 aaaagatcgt gatattttca aggctgttgc cgaattgatg ggggctgagg ttgtaataca
      241 ggcggcgcag ggcaatgacg caacacagat tgcccagtgc gaaaatctga ttactcaggg
      301 cgttgatgtc cttgttatta tccctcacaa cggtgaagca atggctgttg cggttgagaa
      361 agcgcatgaa tctggaattc ctgtcatagc gtatgataga ttgatcatga attgtgatct
      421 tgatatctat atctccttcg ataatgtcag ggttggagag ttacaggcta ccgagataac
      481 caagctagtg cccaaaggaa attatgtcta tattggtgga gctcctactg ataataacgc
      541 tctattgttt aaagagggcg cgatgaatat acttaatcca ttgattgaaa aaggcgatat
      601 aaatcttgtt tttgatcagt tttctgatga ttggtcggca tcgattgcac aaaaacatat
      661 ggaaaacgcg ttaactaaaa acaataacga aattgacgct gttattgcag ccaatgacgg
      721 aaccgcagga ggagccgtgc aggcgctttc cgaacagggg cttgcgggca aggtgccaat
      781 atctggacag gatgccgata tcgcgggttg tatgcgtatc gttgaaggaa cacaatccat
      841 gactgtctat aaatcgataa aatccattgc ggccgaggct gttaaattat caatcagaat
      901 ggcgaagggt gagtcgattg cggctgctaa tacggttaac aacaataaaa aggatgtccc
      961 ctcggttctt ttgactcctg tagctgtgac aaaagagaac atgtatcagg tagttgtaag
     1021 ggatggacat cacaggcttg aagcagtata taaaaatata ccgaaggatc agtggcccga
     1081 ataatcccga ttatagtgtt aataccaatc tgaaaggata gccagatgaa taagacggtt
     1141 tctaccgaaa ttatttctgc ggataataaa aagagtattt tgaatatcaa tatccgtgat
     1201 tatacaatga ttattgctct tgtgttcatc tggatcgttt taactgtatt aacggatggt
     1261 ttttttattg gatcccgtaa tttgtctaac cttatgagat cgatatccgt aacttcggcg
     1321 ctttctctgg gaatttttct gattatagtt gcagggcata tcgatttttc aacaggatcg
     1381 gtttgtgggt ttattggagc tattgccgcc attttgaacg tctggttagg gtggggaaca
     1441 ttcactagta ttgtggtggc gctgtttctt ggaatggtga tcgggtttgc ccaggggtat
     1501 cttgttgcct accagaaagt tcccgccttt atagtaactc ttggagggtt gatgatcttc
     1561 agaggcctac tagtaggagt tacgcgtggt atcaccgttg ctccattaag tgcttctttc
     1621 gttgcgattg gtcagaacta tgtgctgccc gttataggct ggattattgc cggtgtggct
     1681 gtgtctgcta tatgggtgct tgaattacgt acaagaagca ccagattaaa atatggattt
     1741 tccgtaagtt cgttcaagat tgttgttgct aaatcgattt tatactcggg aatagtgttg
     1801 ttgtttgttg caataatgaa taattaccgg ggaatacctc tgccggtatt tattattatg
     1861 ttaattgctt taagtcttgc ttttgttgct tgcagaaccc aattcggacg ttatgtgttt
     1921 gctttggggg gaaatataga cgcggcacgt ctgtcgggca tcaatgtcga gcttacaacg
     1981 atggtgttgt ttgttgtggg agggtttatg tctggtgtag gtggtattat tttaacctcg
     2041 cgtctaaatg ctgccacaac agatgccgga aatatgtacg aatttgatgc tatagcggcc
     2101 tgtattattg gtggtacatc aatcaagggg ggcgcaggaa cggttatcgg tgtaatagtc
     2161 ggagctcttg tgatggcttc gcttgacaat ggcatgagtc ttttaaacac aaataccttt
     2221 tggcagcatg tagtaaaagg cgtaatactg attgcagcgg tgtggtttga tataaatgcc
     2281 aaaaataaaa agatataagg cttaaactta atcaaagtat attttggatt tctgcttgcg
     2341 aaaattctgg ggagaaattt tctctttgga tttgaacatg cgtgagaaat atagaggatc
     2401 actgaaacca acaatctcgg caatttcgtc aatcttcttg tttgttttaa gaagtaactc
     2461 ttttgcttta tcaatcttga gtcttaaaaa gaactgcttg ggagagattc ccgttttttc
     2521 tttgaacatt tttctaaaaa catgatagtg cataccaacc ttggcagcaa gttcgttaaa
     2581 gtcaattgac tcttcgctca aagatttgca cataattttt tttgtgttat agatcggatc
     2641 ataaatcgaa agaatattca gttcttcaga agatgttgcc tggtttctta gctcatctat
     2701 actgcgactt ttatgatctg tgatttcaac aaagccgtct tccgttaaaa aataaaattt
     2761 atccccaaca atttgggact cttccagatt ttcaggaata taaattattc cgtttccggc
     2821 ttttttcttc tcttcaataa tttgttccag tgtttcaaac tggttaaggg taaggtatcg
     2881 agagacttta taaaagatgg atagttttgg tttaagcaga ataatactta ttaggtcaat
     2941 tatagttcgc tcattaagat tatagtcttt tattggtgca cctagatcaa tatcggtatc
     3001 aaaacgttta agaagcgact gtatatcgga aagcaggcgc ttgcggtgta tgattccgaa
     3061 atttgaatag tgctttccgc ccttaagagc aatattgtca aatccgctca aattcataaa
     3121 aagtgatttg ctttgccagg ctattccaat ctggttacct atatggtcac tgataactct
     3181 ttcacctttg aagagaacct cgccactgtc tttcttctcc tggtcagcaa gaaccgagac
     3241 aaaaagccta gccctcgaat cattgtcaga tacaatacag caaatttcat ttgagtgcac
     3301 aaccagattt gactgattaa aacatagtgc gttgtccaca tatttcgtca gatttttaac
     3361 ttttagaagt atcattttct ctattctatt cctgtttata ctctttataa acaatacttc
     3421 tgattttgtc tatcattttg ttaaagatgc ctggattgtt atccacctaa tccggattat
     3481 aggctactct tccggccaaa agatatgata aaatcatcaa tagagttaaa aaaggaagaa
     3541 gataaaggcc gcgggagagt ccgaactgat ggcttatagc acccgcaaag aaaggcataa
     3601 aagcggaacc gatattggag ctggcgtaca atgatcctgt ggcaagatct atttcagaat
     3661 taaaacgctt tataatgccg ttttgaatta tcgggaaaag ccctgaaata gtaagcccgc
     3721 tcaaagggat aaaaacgtaa cagccataaa tagaaacagg taaaatgaat gccggaacta
     3781 caatgagcaa cgttattcct gataaaaggg caaaaagatg ctcctgtttt atacggtgag
     3841 aaatcagagc cgaaacaaga cgtcctgctg taaatagtat aaaaaaagca atcatcacct
     3901 ttgaaatatg gtctttgctt tggccaagaa aatcttccat aaaagttgga gcccagttta
     3961 taatggtaaa ttcgatgcca acataaccta tcgcggccat agcaaaaaac agcataacct
     4021 ttgatgtgat aacaggaaga cgaaatttat gttctttcgg tttctggctg tttttatgct
     4081 cgctgtcggg aaaagttgct atgatcaata taataaaagt taataacgaa acagatgaaa
     4141 aagagagata cacataacgc catgatccgc taaaatcgag tatggtctta gcgaatgcga
     4201 gtgcgattat tgcaccagca ccgaaaaaga agtgaagcaa atgtatcgaa ctttctctgt
     4261 agcgataggc gtgagcttga aaaaaagccg atactccgat atttactccg agtattacta
     4321 ttaatgttgc gaaaccaagt aaagagttaa tggccaaaaa aaagagataa ttaggagaat
     4381 aggctaacgc gacctgaagc agggtaagca aaagtgttcc gcttaaaagg attatcttgc
     4441 gtctcattat tttgtacaga taaccaccaa agaaagagcc gatcaatgct ggtattagcg
     4501 gaaccagaaa cataagccct gatgactggt agttaattga aaaatcgttg ttaatttccg
     4561 gcaataaaaa tcctctgatt gctatggtta tacccatgta tatttgcaga atataggctg
     4621 caagaatcaa aagcatatca cgtttcttca tgaagaattg tatcctttaa ataattgaaa
     4681 tgtttgaaaa acagtctgaa ttattcgata attcagatac cgtgttaatt caccgtacaa
     4741 tcattttata ctagtgaact tcgaaaaatt accatttctg gaacggctat gccgtttaaa
     4801 gcggtatatg tcaacatagt tgcgcctttt tttgaaaaaa tttatgcagc gttttc
//
LOCUS       DAOTGZ010000209         4806 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_3064
            NODE_21034_length_4806_cov_4.865292, whole genome shotgun sequence.
ACCESSION   DAOTGZ010000209 DAOTGZ010000000
VERSION     DAOTGZ010000209.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868088
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 4806)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4806)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:22:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,785
            CDSs (total)                      :: 2,751
            Genes (coding)                    :: 2,732
            CDSs (with protein)               :: 2,732
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 12 of 19
            Pseudo Genes (internal stop)      :: 5 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4806
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_21034_length_4806_cov_4.865292"
                     /isolate="AMR_MDS_3064"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..637)
                     /locus_tag="P5123_11465"
     CDS             complement(<1..637)
                     /locus_tag="P5123_11465"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRX16921.1"
                     /translation="MELIVFNDQESEIIWKCQKEVRDRKNEMALQKINKKINALVELA
                     RAISQYPSIFNSSDINGNVRSAGTLVESLCSREEIDEMMHIPTKAVLGKGYLIAKINF
                     FTMLNQLAMRFPNLHEESSAISQDITEIVFTLMCEDVFMGIIEDKTMDDQIRNRTAFL
                     LANIWEYRLNHGVSEFAPLLSSIWESRKKLRPVFGTMMGTSELMTMSFSVDE"
     gene            complement(744..1034)
                     /locus_tag="P5123_11470"
     CDS             complement(744..1034)
                     /locus_tag="P5123_11470"
                     /inference="COORDINATES: protein motif:HMM:NF012725.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HRX16922.1"
                     /translation="METFLYTRYLIVSDQKTVEGQILNRAVKYILESSYHEAGVLLNQ
                     IKGTFHVAVLNNRAVLNEINGNYDEARNYYHKALELDSGNKVVLYNLNSMRY"
     gene            complement(1164..2615)
                     /gene="gatA"
                     /locus_tag="P5123_11475"
     CDS             complement(1164..2615)
                     /gene="gatA"
                     /locus_tag="P5123_11475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015261769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030956 - glutamyl-tRNA(Gln)
                     amidotransferase complex [Evidence IEA];
                     GO_function: GO:0050567 - glutaminyl-tRNA synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0006424 - glutamyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatA"
                     /protein_id="HRX16923.1"
                     /translation="MITSKSVKQLSDLLNKKEISAVEIANAYIDRINKHNKTINAYIT
                     VNPEKLLINASESDKRRANGNELSEFDGIPIAIKDNICTKGLKTSCASKILGDFVPPY
                     NATVIEKLEQKGLLVLGKLNMDEFAMGSTNETSCYGPVKNPHNIEHVPGGSSGGSAAA
                     VAAHMAPAALGSDTGGSIRQPASFCGVTGMKPTYGRVSRYGLVAFASSLDQIGPLARN
                     IDDAALLTSIISGKDPRDSTSVDPGNKISETIVARDIKTVRIGVPEEYFEGVSDETKH
                     ALSKKINELDSAGAQIVPISLKMTEYAVPAYYLIATAEASSNLARYDGVKYGRRSENA
                     STLDELYKLSRSEGFGAEVKRRIILGTYSLSSGYYDAYYLKALKTRTCIINDFKKAFD
                     KVDIIAAPVVPTTAFRLGENISNPLEMYLSDILTISANLAAIPSISIPIAKDSKGLPI
                     GMQLMGTHFDEKTVFEVGKAIEKDITPLTVSLD"
     gene            2847..4598
                     /locus_tag="P5123_11480"
     CDS             2847..4598
                     /locus_tag="P5123_11480"
                     /inference="COORDINATES: protein motif:HMM:NF012437.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009190 - cyclic nucleotide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0035556 - intracellular signal transduction
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate/guanylate cyclase domain-containing
                     protein"
                     /protein_id="HRX16924.1"
                     /translation="MEKKQTKAYLGLLLTGFSFFALNSGSLLLFLKYYSTVMSMQEYD
                     ELRERFFDELMLLPASTLFLYLAFAFYVVFAGYRIINEAKDASTVILVKKMSQLPSLC
                     VRLTLFIWLGFTCVTGWLLWSFDSQFLLTGRVLLFWSVLSMVVVSVLYYASFYINSVM
                     ILPCIRVGVPPLFDGLYKIRLTLTVKFMLFFAAIALVPLMFLLFLYASLTIQFGRYDL
                     YLNIVVMGVFLTVIPLILSFTFSRSIALPLRKLRKAARDLSKGEYDSPVSIYSGDELG
                     QLSCTINHAAMELKEGQRVKESFGIMVDPVIRDHVLKGNLELGGELKFATVLYTDIRN
                     FTGMSENFPPDFIVEVINSYFDRMSECVTLEHGIVNKFIGDALLAVFGEPVIMEDPVD
                     AALRAAIVMRKDLRLLNESLTERRLPVVDAGLGIHTGDVLAGNIGSKRRMEYTVIGDA
                     VNVATRIEKLTKIYRVPVVISESSYYFLMHKELFNIREIDNVRVKGKQEPVRIFEVFD
                     CDNNEVKKKKIKSLMVFNQAVQFYRDGEFNRALKRFNEICRYNEHDTVSSMYVSRCRR
                     LGSKKMKAWDGVSVYGA"
BASE COUNT         1262 a          860 c         1045 g         1639 t
ORIGIN      
        1 cttcatccac actgaacgac atggtcataa gttcgcttgt gcccatcatg gttccgaata
       61 caggtctaag ttttttgcgg gattcccaga tagagcttaa aagcggtgca aattctgata
      121 ccccgtggtt aagccggtat tcccaaatat ttgctaatag aaatgctgtt ctgtttcgta
      181 tctggtcatc catggtttta tcttcaatta ttcccataaa aacatcttca cacatcagtg
      241 taaatacaat ctcggtaata tcctgtgata ttgcgcttga ttcttcatga aggttgggaa
      301 agcgcatagc aagctgattg agcattgtaa aaaagtttat tttcgctata aggtatcctt
      361 tgccaagaac agctttggta gggatatgca tcatttcgtc aatttcctcc ctgctgcaga
      421 ggctttccac aagggttccg gcgctacgta cgtttccgtt tatatctgac gagttaaaaa
      481 tagaggggta ttgagatatc gctcttgcaa gctcaacaag cgcgtttatt ttcttgttta
      541 ttttttgcaa ggccatttcg ttttttcggt cacgaacctc tttctggcat ttccagatta
      601 tttcactctc ttggtcgtta aagactataa gttccatttt aattcctaat ccggctttat
      661 aatgacaaag ccgtttgtaa tatggtaaat cgtcgatgat tctttgtaat caagtcaaat
      721 gataattcct tatcaaaaga tgattaatat ctcatactgt tcagattata tagtaccact
      781 ttattgccac tgtcaagttc caaggctttg tggtaatagt ttcgcgcctc atcatagttg
      841 ccgtttattt cgttaagaac tgctctgttg ttcagtacgg ctacatgaaa agttcctttt
      901 atctgattga gtagtactcc cgcttcgtga taactgcttt ccaatatgta tttgaccgcc
      961 cgatttagta tctgaccttc aacagttttc tgatcgctta caataagata gcgcgtatat
     1021 agaaacgtct ccatttttta cggttatttt cggcagaaga aacatattct ccaatgccga
     1081 taattccgtc gctgtagtat cggtcactga ttcttaccgt ggatgtttta acatccgaag
     1141 aacccgagca gcttataaca atgctaatcg agactaactg taagaggtgt aatgtctttt
     1201 tcaattgcct tgccaacttc aaatacggtt ttttcatcaa aatgagtacc cataagctgc
     1261 attccgattg gaagtccttt agaatcttta gctattggta tgcttatact tggtattgcg
     1321 gcaagattcg ccgaaatagt caaaatatcg gaaaggtaca tctctagtgg gttgcttatg
     1381 ttttcaccca gcctaaacgc ggtagtagga acaaccggtg cagcaataat gtctacttta
     1441 tcgaaagctt tcttaaagtc attaatgatg catgttcgtg ttttaagggc ttttaaatag
     1501 taggcgtcgt agtagccaga tgaaagtgaa taggttccaa gaattattcg tcttttaact
     1561 tctgctccaa aaccttcact tcggcttaat ttgtaaagtt cgtcaagtgt tgaggcgttt
     1621 tcacttcgtc tgccgtactt tacaccatcg taccgagcca ggttcgatga tgcttcggcg
     1681 gtagctataa gatagtaggc gggtacggca tattcggtca ttttaagcga aattggtaca
     1741 atttgcgctc cggccgagtc aagctcgttg atcttttttg acaatgcgtg tttcgtttcg
     1801 tccgaaactc cttcgaaata ctcttcggga acgccaattc ttactgtttt aatgtctctc
     1861 gcgacaattg tctctgatat tttgttacct gggtcaacag aggtcgagtc tcttggatct
     1921 ttgccgctaa taattgatgt taaaagagcc gcgtcatcga tattgcgggc tagaggacca
     1981 atttgatcta gagaggaggc gaatgctacc agtccataac gggatactct tccatatgtt
     2041 ggcttcatgc ccgttacccc gcaaaaagat gctggttgcc tgatagagcc tcctgtatca
     2101 gaaccaagag ctgcaggtgc catatgagct gctactgctg cggcagagcc tccagagctg
     2161 ccgcctggaa catgttctat attatgagga tttttcactg gaccataaca tgatgtttca
     2221 tttgtagaac ccatcgcgaa ctcgtccata ttcagtttgc cgaggaccaa aagtcctttt
     2281 tgttccaact tttcaattac agtggcatta tatggaggaa caaagtcacc aagaattttt
     2341 gaggcacagc tggttttaag gccttttgta caaatattgt ctttgatggc aatgggtatg
     2401 ccatcaaatt cggatagttc atttccattt gcgcgccgtt tatcggattc ggacgcgtta
     2461 atcagtagtt tctctggatt aacagtaata tacgcgttta ttgtcttatt gtgcttgttt
     2521 atgcggtcta tataggcatt cgcgatctca actgcggata tctctttttt attaagcaga
     2581 tcagatagtt gttttactga ttttgatgta atcatcttga gctcctggtt ttgtaataac
     2641 ggtcgaaatg accgaattat tgaagaaata gtgcgaagaa cggcggctca aggtcaatga
     2701 cttttttggg aagaccgttt ttaagtttcg cgtagctgtt tttataccga tctttgttat
     2761 ataatgagaa actgagcatt gattattgaa cgttgtctga atatcacggt tgctcaattc
     2821 taattctgaa ataactggag tatttagtgg aaaaaaaaca aactaaggca tacttgggtt
     2881 tacttttgac cggattctcg ttttttgctc ttaatagtgg tagccttttg ctgtttttaa
     2941 aatattacag tacagtaatg tctatgcaag agtatgatga gttgagagag cgttttttcg
     3001 acgagttgat gttgttaccg gctagcaccc tctttcttta tctcgcattc gccttttatg
     3061 tcgtgtttgc aggctatcga atcattaatg aggcaaagga tgcatctacg gtaattctgg
     3121 tcaaaaagat gtcccaattg ccgtcacttt gcgttagatt gacgctcttt atttggctgg
     3181 gttttacttg cgtgacagga tggttactct ggtcttttga ctcacaattt cttttaacgg
     3241 gaagagtctt gcttttttgg tctgtattgt ctatggttgt ggtttcagtt ctgtactatg
     3301 cttcgtttta tataaattct gttatgattt taccgtgtat cagagtagga gttcccccgc
     3361 tttttgacgg tttgtacaaa ataaggttaa cccttactgt taaatttatg cttttctttg
     3421 cggcaattgc tcttgtgccc ctgatgtttc ttctttttct atatgcttca cttacaattc
     3481 agtttggtcg ttatgacctt tatttaaata ttgttgttat gggagtattt ttgacagtta
     3541 ttccgttaat cttgagtttt actttttcac gttctatcgc attgccttta cgtaaactgc
     3601 gaaaggcggc tcgggatttg tcaaaagggg aatatgattc tccggtttct atctattcag
     3661 gtgatgagct ggggcagttg tcttgtacaa taaatcatgc cgccatggaa ttgaaagagg
     3721 ggcagagagt caaagagagt tttggaatca tggtggatcc ggtaatacgt gatcatgtgt
     3781 taaaaggtaa tttggagctt ggaggtgagt tgaaatttgc tacggtattg tatacagata
     3841 taagaaactt taccggaatg tctgaaaatt ttccaccaga tttcattgta gaggtaatca
     3901 atagctattt cgatcgaatg agtgagtgtg tgactctcga gcatggaatt gtgaacaagt
     3961 ttataggaga cgcgttactt gctgtttttg gagagcctgt gattatggaa gatccggtag
     4021 atgcagcact tcgtgccgca atagttatga gaaaggatct aaggctgctt aacgagtctc
     4081 tgactgaacg aagattgcct gttgttgatg cagggcttgg gatacatact ggtgatgttc
     4141 ttgccggtaa tattggttca aaacgaagaa tggagtatac ggttatcggt gatgctgtaa
     4201 atgtagcgac ccgtattgag aaattaacaa aaatataccg tgtaccggtt gtaataagtg
     4261 aaagttcgta ctattttttg atgcataagg agcttttcaa tattagagag attgacaatg
     4321 tgagggtcaa ggggaagcag gagccggtga gaatttttga ggttttcgat tgtgataaca
     4381 atgaagttaa aaagaagaag atcaaaagtt taatggtttt taaccaggct gtacagtttt
     4441 acagggatgg agagtttaac agggctttga aacggtttaa cgaaatttgc agatataatg
     4501 agcacgacac tgtttcgtcg atgtatgtgt cccgatgcag gcggttaggg tcaaaaaaaa
     4561 tgaaagcctg ggatggagtt tcggtgtatg gagcttagaa gaaagtagta aagttatatg
     4621 actgtgaatt aataaataat aataataaat taatgattaa ttctgttgac accattaagg
     4681 gtaatattta atgtcacatc acatcagagg aaattgcttc tttttttttc tcccatcttg
     4741 attttttcaa gatttttgtg taattttatt gacagagaaa agcagttttg tcataacggt
     4801 tcacgc
//
LOCUS       DAOTGZ010000210         4796 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_3064
            NODE_21090_length_4796_cov_5.170639, whole genome shotgun sequence.
ACCESSION   DAOTGZ010000210 DAOTGZ010000000
VERSION     DAOTGZ010000210.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868088
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 4796)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4796)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:22:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,785
            CDSs (total)                      :: 2,751
            Genes (coding)                    :: 2,732
            CDSs (with protein)               :: 2,732
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 12 of 19
            Pseudo Genes (internal stop)      :: 5 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4796
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_21090_length_4796_cov_5.170639"
                     /isolate="AMR_MDS_3064"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1031
                     /locus_tag="P5123_11485"
     CDS             <1..1031
                     /locus_tag="P5123_11485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014625675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HRX16925.1"
                     /translation="QSGNPPDLFRSWGGGVMNMYAESGLLRDITDELKKDDFIDTISE
                     GPLGVYSYKGKVYGIPYTMGAVGLWYNKKIFKDNGIEPFKTWDDLIAGCKKLKAAGIT
                     PIALGGNDKWPGHFWWVYLAVRTGGKEAFDKAYGRDGSFTDEPFIEAGRLLKQLVEIE
                     PFQKGFLGATYTDQSTLVANGKAAMELMGQWAPSVQAQNTDDKKGLGSDLGFMPFPAV
                     NGGAGKITDAMGGGDGLAVGKNAPDETIEFLKFMMSGDNYKELMSSPISILPTVKGAD
                     KYITDPQEKAVAEMTAKAEYYQLYYDQYLPPAVGEVVKDATQGIFAGTKTPEEAASMI
                     EKAMKEEN"
     gene            1229..2143
                     /locus_tag="P5123_11490"
     CDS             1229..2143
                     /locus_tag="P5123_11490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013967789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HRX16926.1"
                     /translation="MNNKINRSGYFSKGLFSRNSLTAFAFLAIPLAVYTIFVIYPVLQ
                     AIWFSMFKWDGLGPMTDFVGAKNFLKIFADPVFRIAIGNNFKIVFLSLFIQLPLALFI
                     ALIIARKFKGAVVYRAIFFLPYILSEVIAGVLWSFIYNPQFGIQNTILVKWIPFLADF
                     PFLGSPTTVFYSIFFVMIWKYFGLHMVLYIAGLQNIPQEIEEAARLDGVNWWQMNLHI
                     ILPLLKPTIFLSIFFSIVGSFQTFDIVWAMGKGEPVNAAETMVTYMYKFGFQRFQLGY
                     GSAVAIVIFLITLIVGLVYQKLTASKDI"
     gene            2154..2993
                     /locus_tag="P5123_11495"
     CDS             2154..2993
                     /locus_tag="P5123_11495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013967790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HRX16927.1"
                     /translation="MIIGENRAVVSFKYLLMTVFAILTLLPIILVVVGGFKTLAQLRL
                     DLFGIPDPVMWSNYLDVVNPERSMFFVNLLNSCIVMFITVALGLILSSLAGFALSRIK
                     FKGKEIVFTFFLLGLLFPPAVAILPLYIELKTLSLLNNYMGVVLPQVAFALPFQIMLT
                     RSFFNQIPIELEESAYLDNCTPLGFLIHIVMPLSKPILTTVALLAMVTSWNNYFLPLL
                     VFNDEKLFTLPMGVMSFQGQYINDWSLVLAFISLAMIPAIIFYLIAQKYIIDGLTGGA
                     VKQ"
     gene            3023..4525
                     /locus_tag="P5123_11500"
     CDS             3023..4525
                     /locus_tag="P5123_11500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019551824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-N-arabinofuranosidase"
                     /protein_id="HRX16928.1"
                     /translation="MKQAHCLIDKNYIIDDINDELFGSFIEHLGRAVYTGIYEPKHPT
                     SDSKGFRTDVIKLVKELDVPIVRYPGGNFVSGYNWTDGIGPVKSRPKRLDLAWHTIEN
                     NSFGIDEFADWAREAGTEIMAAVNLGTGTPQDAGYMVEYCNHPNGTYWSDLRVQNGHT
                     EPHNIKTWCLGNEMDGHWQICQLSAEDYAKKAREAAKIMKWVDPSIKLVACGSSSSRM
                     PSFPDWDRIVLEYLYDHVDYLSLHSYYEKESTTEDFLASFANMDYLIRTVGGVIDFAK
                     AKTGSKKDVKLSFDEWNIWYQKKQSDHDWQNAPVMFEESYSLLDSLVMGGLLCTLLNN
                     CDRVKMASLAQLVNVIAPITTKPGGGILKQSTYYPFLQVSKYGRGKALKVLSRTPVIE
                     TKQWGEAPLLQIAATMNEETESVTLFVLNTDVNDSINLTLDMRSFGKTEIIEHLIMNG
                     DNLYAANTFENPETVVPKYVPTDTHTNDGGVFNLNLQKLSWNVIRFTIPK"
     gene            complement(4604..>4796)
                     /locus_tag="P5123_11505"
     CDS             complement(4604..>4796)
                     /locus_tag="P5123_11505"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRX16929.1"
                     /translation="WETILYDYTHSYSGGTLVIASQSSPLKTYVNFEVSSLTLSDLES
                     YTESSDDVSRILLMMKTAE"
BASE COUNT         1431 a          960 c          980 g         1425 t
ORIGIN      
        1 tgcagtcagg aaacccacca gatcttttca gaagctgggg cggcggcgtt atgaacatgt
       61 atgccgaatc tgggttgctt cgcgatatta cagacgaact gaagaaagac gattttattg
      121 acacaatatc cgaaggtccg cttggtgttt acagttacaa aggcaaagtt tacggaatac
      181 cctacaccat gggtgcagtt ggcttatggt acaacaaaaa gatatttaaa gacaacggca
      241 tagagccttt taaaacatgg gacgacctaa tagccggatg taaaaaactg aaagctgccg
      301 gtatcacacc aatcgctctt ggtggaaacg acaaatggcc cggacatttc tggtgggttt
      361 atcttgcggt tagaaccggc gggaaagaag cttttgacaa ggcttacgga agagacggat
      421 cttttactga cgaaccgttt atagaagcgg gaagacttct taaacagctt gtcgaaattg
      481 aaccatttca aaaaggattt ttgggcgcga cctatacaga tcagtcaacc cttgttgcca
      541 acggaaaggc cgcaatggaa cttatgggac aatgggctcc aagcgtacag gctcaaaaca
      601 ccgacgacaa gaaaggcctt ggaagcgacc tgggtttcat gcctttccct gcagttaatg
      661 gcggagctgg caaaatcacc gacgccatgg gcggaggaga tggtttagcc gtaggtaaga
      721 acgcacctga tgaaaccatt gaatttttga aatttatgat gagtggcgac aattacaaag
      781 agcttatgag ttcaccgata tctatcttac ctacagtcaa gggtgccgat aaatatatta
      841 ctgacccgca agaaaaggct gttgctgaaa tgacagcaaa agccgaatac tatcagcttt
      901 actatgatca gtatttacca ccggctgttg gtgaagtagt aaaagatgcc actcagggaa
      961 tttttgccgg aaccaagact cctgaagaag cagcatcaat gattgaaaaa gcaatgaaag
     1021 aagaaaatta aaacctccat ttaattatac aatcatctcc gaagacaggg tgagattacc
     1081 accttgtctt tgggatgaca aatggaatca atttaccaca ttacaggggc gatttttaat
     1141 ttgctttcgg atcgcccctt aatattacaa tttcaagatt ttctgtaacc aatccagtta
     1201 ccggtttgca agatctggag tatataacat gaacaataaa attaacagat ccggatattt
     1261 ttcgaaaggt cttttctcaa gaaactcgtt aacagcattt gcctttcttg caataccgct
     1321 tgctgtttat acaatatttg ttatttatcc ggtattacaa gctatctggt ttagtatgtt
     1381 taaatgggac ggtctaggcc ctatgacaga ttttgtgggg gcaaaaaact ttttaaaaat
     1441 ttttgctgat ccggtattca gaatagctat tggaaataac ttcaaaatag tttttctatc
     1501 gctttttata caattaccac ttgccctttt catcgctcta ataattgcca gaaaatttaa
     1561 gggggcggtt gtttatcgcg ctattttctt tttaccatac atcctctccg aagttattgc
     1621 cggagtttta tggtctttta tctataatcc tcagtttgga attcaaaaca ccatactggt
     1681 caaatggatc ccatttttag cagatttccc ctttttaggt tcgcctacaa cagtttttta
     1741 ttcgatattc tttgttatga tatggaaata cttcggcctg catatggtac tttatattgc
     1801 cggtcttcaa aatataccac aggaaataga agaggcggct cgccttgacg gagttaactg
     1861 gtggcaaatg aacctgcata ttatactgcc tcttttaaag cctaccattt ttctttcaat
     1921 attcttttca attgttggat cttttcaaac ttttgatatt gtatgggcta tgggcaaagg
     1981 tgagccggtt aacgcagccg aaacaatggt aacctacatg tataaatttg ggtttcaacg
     2041 ttttcagctt ggatacggaa gcgcggtcgc tatcgtaatt tttctaatta ctcttatagt
     2101 tggacttgta taccaaaaat taaccgcatc aaaagacata taaggaaagt aaaatgataa
     2161 taggtgaaaa cagagcagta gtgtctttta aatatttatt gatgacagtg tttgcaattt
     2221 taacactact tccgataata cttgttgtag tcggtggttt taaaactctc gcgcagttac
     2281 ggcttgatct ttttggcatt ccggatcctg ttatgtggag caactatctt gacgtagtta
     2341 atccagagcg atccatgttc tttgtgaatc ttcttaactc ttgcatagtt atgtttatta
     2401 ctgtcgcttt aggactaatt ctctcgtcac ttgctgggtt tgccctttct agaattaagt
     2461 ttaaaggaaa agagattgtt tttacttttt ttcttcttgg acttcttttc cctccggctg
     2521 tcgccattct tccgttatat atagaattaa aaacacttag cctgttaaac aattatatgg
     2581 gtgtagtatt accccaggtt gcgttcgctt tgccgtttca gattatgtta accagaagtt
     2641 tttttaatca gattccgata gagctggaag aatcggcata tcttgacaac tgcactcctc
     2701 ttggttttct tattcatatt gttatgcctt tatcaaagcc gatactaacg actgttgccc
     2761 tgcttgctat ggttacaagc tggaacaact attttttacc actactcgtt tttaatgatg
     2821 aaaaattatt tacactgcca atgggggtaa tgtcgtttca gggacagtat atcaatgact
     2881 ggtctctcgt tcttgccttt atctcgttgg caatgatacc ggcaattatt ttttatctga
     2941 tcgcacaaaa atacattatt gacggactaa ccggcggagc cgtgaagcaa tagtcaatat
     3001 ataactatca aggataccaa tcatgaaaca agcacactgt ttaattgaca aaaattacat
     3061 aatagatgat atcaatgacg aattatttgg atcatttatc gaacatcttg gacgtgcggt
     3121 gtacaccgga atatacgaac caaaacatcc aacttccgat agcaagggat tcagaaccga
     3181 cgttattaag cttgttaaag aactcgatgt tccgattgtt cgctatcccg gcggcaactt
     3241 tgtttcaggt tacaactgga ccgacggaat tggccccgta aaatcacgcc cgaaacgtct
     3301 tgatctggct tggcatacta ttgaaaataa tagttttggc atagatgagt ttgccgattg
     3361 ggctcgtgaa gccggtacag aaataatggc agccgtgaat cttggaaccg gcactcctca
     3421 agacgcaggc tatatggtcg aatactgcaa ccatccaaat ggcacatact ggagcgattt
     3481 acgcgtccag aacggccaca cagagcctca caacattaaa acctggtgtc ttgggaacga
     3541 gatggacgga cactggcaaa tttgtcagct atcagcagaa gattacgcca aaaaagctcg
     3601 tgaagccgcc aagatcatga aatgggtcga tccgtcaata aaacttgtag cctgcggcag
     3661 ctcgtcctca cgaatgccca gttttccaga ttgggatcgg attgttctgg aatatcttta
     3721 tgaccatgtc gactaccttt cacttcacag ttattacgaa aaggagtcaa caacagaaga
     3781 ttttttggcg tcgtttgcaa atatggatta cctgatccgc acagtgggtg gcgttatcga
     3841 cttcgccaaa gcaaaaaccg gaagcaaaaa ggatgtgaag ttatcttttg acgaatggaa
     3901 catctggtat cagaaaaaac agtcagacca tgattggcaa aacgcacctg taatgtttga
     3961 agagagctac tcgttgcttg actcactggt aatgggcggt cttttgtgca ctctattaaa
     4021 caattgcgac cgtgttaaaa tggcaagcct ggcacagctt gtaaatgtta tagctcctat
     4081 aaccactaaa ccaggcggag gcatcttgaa acagagtacc tattaccctt tcttgcaggt
     4141 ttcgaaatat ggcagaggca aggctttaaa agtgctttca cgtaccccag ttatagagac
     4201 caagcagtgg ggtgaagcgc ccctgctcca aattgccgca acaatgaatg aagagactga
     4261 atctgtaacc ctgtttgttc taaacacgga tgttaacgat tccattaatc ttacactgga
     4321 tatgcgctct tttggtaaaa ccgaaattat cgaacacctg atcatgaatg gtgataatct
     4381 ttatgcggct aacaccttcg aaaaccctga aacagttgtt ccaaaatatg tccctacaga
     4441 tacacataca aatgacggtg gagttttcaa cctgaacctg caaaagctgt catggaatgt
     4501 aatcagattt acaattccaa aatgaatttg agaatattac tacgatacaa aacagcattt
     4561 tattaaaatg ctgttttgta aacctgaagc agttattttg tacttattca gcagttttca
     4621 tcattaaaag aatcctgctg acatcatcac tcgactcggt ataactttca agatcagata
     4681 aagttaatga agaaacttca aaattgacat aagtttttaa agggctactt tgtgaagcaa
     4741 taacaagcgt tccacccgaa tagctgtgag tatagtcata taatattgtc tcccag
//
LOCUS       DAOTGZ010000211         4793 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_3064
            NODE_21107_length_4793_cov_5.347826, whole genome shotgun sequence.
ACCESSION   DAOTGZ010000211 DAOTGZ010000000
VERSION     DAOTGZ010000211.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868088
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 4793)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4793)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:22:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,785
            CDSs (total)                      :: 2,751
            Genes (coding)                    :: 2,732
            CDSs (with protein)               :: 2,732
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 12 of 19
            Pseudo Genes (internal stop)      :: 5 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4793
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_21107_length_4793_cov_5.347826"
                     /isolate="AMR_MDS_3064"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..973
                     /locus_tag="P5123_11510"
     CDS             <1..973
                     /locus_tag="P5123_11510"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF022137.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HRX16930.1"
                     /translation="YKKRYVRLLRLLRQPFFMEYLAILKSAYRPAIDNCGRVFNGNRF
                     GYPGITIDRYNNTLLVQFFDKDYLDNRDKICDSILQFFSNIFEVDALWYKDRTILKDV
                     KMISKIRESVLYYGNNERFAEVKHNGCIIEVDFVSGQNPGIFADMREVRDRLPSYYSE
                     VDSMLNLFCYTSVFSVHALLNGVKNAVNVDTSKQVLERSKINYRKNSLGIDNRDFIHN
                     DSGLYLEKLVKYGRKFDIVIYDPPTFSRNKKFYFSVKNDYKKQLNLISQVSNRYVLSA
                     INSYSVSQQEYLDYHPNKWEMLFFMNEASDFHDPSDPYLKCALWQIS"
     gene            1197..>3100
                     /locus_tag="P5123_11515"
     CDS             1197..>3100
                     /locus_tag="P5123_11515"
                     /inference="COORDINATES: protein motif:HMM:NF009381.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor G"
                     /protein_id="HRX16931.1"
                     /translation="MVKKYAKENIRNVVIVGHAGTGKSTLMDSILFTGNKIKKIGSHN
                     DGSLISDFDIEEKKKKMSVHTAMGFVETEDIKINIIDCPGHADLIGERRAAIQAADAA
                     ILVLDSVDGVQVGTEKVWRYLAENNIPRIIFVNKMDQERASYENVIASMEATLHANLV
                     VAAIPISEGDSFDGVVDIIEMKSMKQKAPGSNEIIRGEIPADLVDKVQEQRSRTVELA
                     AEGDDELIEMFLEGKDFDADKIYRGINEQIRQNKLHPVICGAALKGVGVQTLLHFIKE
                     FMPSPAEKAEMEALDLDKNASPIKIKCNEKEPLTAVVWKTYIDQFAGKFSYFRVISGE
                     VTSEIELMNTDCKEKEKISKIYTMIGKELVEVEGLSTGDIGVFVKLDKTITTDTLCDL
                     SRNIKVPLINLPGPVFGYSIKAKDKKDEEKLGQILNRYDEQYPTLHYSYNSETRQATI
                     YGMGQLHLDITLDEIHEKYKIEFETDVPRISYCETITKKAEAQYKHKKQSGGHGQYGE
                     VYLRLHPKPRGEGYSFKESIVGGVVPKNYIPGVEKGVLEALKEGVVARYPVVDVEVEL
                     FDGSFHPVDSSEMSFKIASQHAFRKAMSDAAPVILEPIMHVSVYVDNQFVGDVMGDIT
                     SRRGRVLGMDS"
     assembly_gap    3101..3110
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <3111..3305
                     /locus_tag="P5123_11520"
     CDS             <3111..3305
                     /locus_tag="P5123_11520"
                     /inference="COORDINATES: protein motif:HMM:NF009379.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRX16932.1"
                     /translation="NKESITRIRALIPHAELLRYSIDLTALTQNKAVFEMEFHGYEPI
                     SGRVADKVIADRESMLAEES"
     gene            3289..4554
                     /locus_tag="P5123_11525"
     CDS             3289..4554
                     /locus_tag="P5123_11525"
                     /inference="COORDINATES: protein motif:HMM:TIGR03991.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative sugar nucleotidyl transferase"
                     /protein_id="HRX16933.1"
                     /translation="MQKSPERIVVTNYGQNKYNPISQTRPVWDILCGCLYAFERIKIL
                     CDNKYPGIDLVFLCSRDMLNFCKEKHPSLDIVDTIADGKATLFLNPFLYDIDFIDMEI
                     QNCVYLNGNTFLAAYTDSANSLRFSPDATDNRLDIVESEIIVPDYIWDMVNQNGFYIH
                     NDISLLKKKNSVTVDYDSLTIVGSSENLYIDNEAVIDPFVVIDTRKGPVYISGGVTIN
                     SFTRVEGPCYLAPGVILYGAKIREGCSIGEGCRIGGEVEDSVFHPWSNKYHDGFIGHA
                     CIGEWVNLGALTTNSDLKNNYSSVAVYQNGNIINTGLVKVGCFIGDHTKMSIGSLINT
                     GASIGCFSMSVHSGRMTPQFIPSFSIFIKNGLRDIKSVETLLDSTEIVMNRRNKSMSN
                     AFKEYIINLYKNNERERVLGLDRWNKSVI"
     gene            4533..>4793
                     /locus_tag="P5123_11530"
     CDS             4533..>4793
                     /locus_tag="P5123_11530"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRX16934.1"
                     /translation="MEQKRDIKFHFFIIVALVVHIYIASFIIMPYYFASYTNSSYVEQ
                     LFKDIISNSTELRDVVVNINPDDQEVMTNETLLSDVTSSAKGK"
BASE COUNT         1561 a          790 c         1016 g         1416 t
ORIGIN      
        1 gtataaaaag aggtatgtaa gacttctaag gctgcttcgg cagccttttt ttatggaata
       61 tcttgcgata cttaaatctg cttatagacc ggctatagat aattgtgggc gtgttttcaa
      121 tggtaacagg tttggttatc caggaattac catcgataga tataataaca cacttctggt
      181 gcagtttttt gataaagatt atttggataa tcgggataaa atatgtgatt cgattcttca
      241 gtttttttca aatatctttg aagtagatgc cctctggtac aaagatcgaa caatattgaa
      301 agatgttaag atgatctcta aaattcggga aagtgtacta tattatggta ataatgagcg
      361 attcgcggaa gtaaaacaca acggttgcat tattgaagtg gattttgtgt cgggacaaaa
      421 ccccggcatt tttgctgata tgagagaagt tagagaccga ttacccagtt actattcaga
      481 agtcgattca atgcttaacc tgttttgcta tacttccgta ttctctgttc atgctctttt
      541 gaatggagta aaaaatgctg taaatgtaga tacaagtaaa caggtattag aaagatccaa
      601 gataaattac agaaaaaaca gccttggtat cgataataga gattttattc ataacgactc
      661 ggggctatac ctggaaaaac ttgtgaaata tggcagaaag tttgatattg tgatatatga
      721 tcccccgact ttttcacgta acaaaaagtt ctatttttct gtaaaaaacg attacaaaaa
      781 acagttaaac cttatatctc aggtctcaaa ccgttacgta ctttcggcga ttaactcata
      841 ttccgtcagt cagcaggaat atcttgatta ccatccaaac aaatgggaaa tgcttttttt
      901 tatgaacgaa gcgtcagatt ttcatgaccc atccgatcca tacctaaaat gcgcgttatg
      961 gcaaattagc taaaaaaata cggacaattc agaagaataa cagttgtgtt ttgccggtgc
     1021 aattttataa aaaagagtcc gcttttgaaa attcctttac atttttacag ctcaattatt
     1081 aaataagtaa gcatcctcgc tgtataattt ttgaacaaat ttgcgataat ttaccgcact
     1141 ctttacagta gtgtatcaga ttattgcggt tatataaaat caaaatcgga ggaattatgg
     1201 taaagaaata cgctaaagaa aacatccgta acgtcgttat tgttggtcac gccggaacag
     1261 gtaaatccac attgatggat tctattcttt tcactgggaa caagataaaa aaaatcggct
     1321 cacataatga tggctcacta atctctgact ttgatattga agaaaaaaag aaaaaaatgt
     1381 cggttcacac agctatgggg tttgttgaaa cagaagacat aaaaataaac attatagatt
     1441 gtccggggca tgcggatttg atcggagaaa gacgagcagc gattcaggcg gctgacgcgg
     1501 ccatacttgt tcttgattcg gtagacggcg ttcaggtcgg aacggaaaaa gtatggcgct
     1561 atctcgcaga aaacaatatt ccaagaatta tttttgtcaa taagatggat caggagagag
     1621 catcttatga aaacgttata gccagcatgg aagcgaccct tcacgctaat cttgtagttg
     1681 ccgcgattcc aataagtgag ggtgactcat ttgatggtgt agtagatata attgaaatga
     1741 aatccatgaa acaaaaagca ccgggttcta atgaaataat tcgcggcgaa atacctgcag
     1801 atcttgtcga caaggttcaa gaacagaggt cacgcactgt agaacttgca gcagaaggtg
     1861 atgacgagct gatagagatg tttctagaag gaaaggattt tgacgctgac aaaatatatc
     1921 gaggtataaa cgaacagata cgccaaaaca aacttcatcc ggtaatctgt ggggctgccc
     1981 ttaaaggagt cggggttcag actctactcc attttataaa agagtttatg ccatcaccag
     2041 cggaaaaagc cgagatggag gcacttgatc ttgataaaaa cgcgtcacct ataaaaatca
     2101 aatgtaatga aaaagagcca cttactgcgg tcgtctggaa aacctatatt gatcagttcg
     2161 ctggcaagtt ctcttatttc agggttatat cgggagaggt aacatctgaa atagaattaa
     2221 tgaataccga ttgtaaagag aaagagaaaa ttagcaagat ttacacaatg ataggcaaag
     2281 aactagtaga ggtagaaggg ctttcgacag gcgatatcgg tgtttttgtg aagcttgata
     2341 aaacaattac tacagatact ctatgtgatc tatcacgtaa tatcaaggtt ccattaatca
     2401 atttgcctgg acctgttttc ggttattcaa tcaaggctaa agataaaaaa gatgaagaga
     2461 agcttggaca gattcttaac agatacgatg agcaatatcc aacgctccat tatagttata
     2521 attctgaaac caggcaagcg actatttacg gtatgggaca attacatctt gatattacat
     2581 tggatgaaat tcatgaaaaa tataaaattg aatttgaaac ggatgttccc cgcataagtt
     2641 attgtgaaac aataacaaaa aaagcagaag cacagtataa acataaaaaa cagtcgggtg
     2701 gtcatggaca gtatggagag gtctatctaa gacttcatcc aaagccccgt ggtgaaggct
     2761 actcctttaa agagagcatt gtcggcggtg ttgttcctaa aaattacatc ccaggtgtcg
     2821 agaagggggt tcttgaagct ttaaaagagg gtgtagttgc aagatatccg gttgttgatg
     2881 ttgaggttga actgtttgac ggatcttttc acccagttga ctcgtctgag atgtcattta
     2941 aaatagcttc gcaacacgca tttagaaagg caatgagtga tgcagcacct gtgattctgg
     3001 aacccataat gcatgtcagt gtctatgtag ataatcagtt tgtaggtgat gttatgggtg
     3061 atatcacctc aaggcgagga cgtgttcttg gtatggattc nnnnnnnnnn aataaagaga
     3121 gtattacgcg cattagggcg ttaatacctc atgctgaact gttacgatac agtattgatc
     3181 ttacagcgtt gactcaaaat aaggctgttt tcgagatgga atttcacggt tatgaaccta
     3241 tcagtgggcg tgttgctgac aaggttatag ccgacaggga gagcatgctt gcagaagagt
     3301 cctgaaagaa tagttgtcac taattacgga caaaataagt acaacccaat atcccagacc
     3361 agaccggttt gggatattct ttgcggttgt ctctatgctt ttgaacgtat aaaaatattg
     3421 tgtgataata aatatcctgg gatcgattta gtgtttctat gttccagaga tatgttaaat
     3481 ttttgcaaag aaaagcatcc ctctctagat attgtggaca caatcgcaga tggcaaggcg
     3541 acgctttttt tgaatccgtt tttatatgat attgatttta tcgatatgga aattcaaaac
     3601 tgtgtctatc tgaatggaaa tactttttta gctgcctata cggattcggc taacagctta
     3661 aggttttcac cggatgcgac agataacaga cttgatatcg ttgaatccga aattatcgtt
     3721 cccgattata tctgggatat ggtcaatcaa aacggatttt acatacataa cgatatctct
     3781 ctattaaaaa agaaaaatag tgttactgta gattatgatt cgttaacaat tgttggctcc
     3841 tccgaaaatc tgtatattga taacgaagcg gtcatcgatc cttttgtagt aatagataca
     3901 cgaaaaggac ctgtttatat atcaggggga gttactataa attcgtttac cagagttgaa
     3961 ggcccttgtt atcttgcacc cggcgttatt ctttacggag cgaaaatcag agagggttgt
     4021 tctatcggtg aaggctgccg tatcggtgga gaagtggagg attctgtctt tcacccatgg
     4081 tcaaacaagt accatgacgg ttttatcggt catgcttgta taggcgagtg ggtcaatctg
     4141 ggtgcattga ctactaacag tgatcttaaa aataattatt cttcagttgc ggtttaccag
     4201 aatggaaaca taattaatac gggactggtc aaagtaggat gttttatagg tgatcacaca
     4261 aagatgagta tcggctcact tattaatacg ggggcttcta ttggctgctt ttcgatgtca
     4321 gttcactctg gcagaatgac acctcagttt ataccttcgt tttcgatatt tataaaaaat
     4381 ggccttaggg acataaaaag tgtcgaaaca ttgcttgatt cgacagaaat tgttatgaac
     4441 agacgtaaca aatcaatgtc taatgcattc aaagagtata taattaatct ttataagaat
     4501 aacgagcgag aaagagtgct cgggctggat agatggaaca aaagcgtgat ataaaatttc
     4561 acttttttat aattgttgcg cttgtagtac atatttacat cgcatccttt ataataatgc
     4621 cgtattattt cgcaagttat acaaacagta gttacgtcga acagcttttt aaagatataa
     4681 tcagtaattc tacggaatta agagatgttg tcgtaaatat caatcctgat gaccaagagg
     4741 ttatgacaaa tgagacttta ctttctgacg taacctcaag tgcaaaaggg aaa
//
LOCUS       DAOTGZ010000212         4753 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_3064
            NODE_21325_length_4753_cov_4.542358, whole genome shotgun sequence.
ACCESSION   DAOTGZ010000212 DAOTGZ010000000
VERSION     DAOTGZ010000212.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868088
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 4753)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4753)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:22:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,785
            CDSs (total)                      :: 2,751
            Genes (coding)                    :: 2,732
            CDSs (with protein)               :: 2,732
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 12 of 19
            Pseudo Genes (internal stop)      :: 5 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4753
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_21325_length_4753_cov_4.542358"
                     /isolate="AMR_MDS_3064"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(68..1747)
                     /locus_tag="P5123_11535"
     CDS             complement(68..1747)
                     /locus_tag="P5123_11535"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRX16935.1"
                     /translation="MMISANFKHKNKLFSAFLSLLFTFTVAGIPGPEYDVRVSGIRFL
                     YGTGVGPTHSGLYNSSNYYRSYGQSGTLIELTAQMNVSGWTHMYGPVAFTVSFSNGSN
                     TASFDVTIPSGQNRVIALVPFSDASGFPDSFITNGQTIPFEYTVTAASGQHADNLISS
                     VSQTIYWDRNDPPQSSVFPPADETEISVSLTTNSARIMWSPLVTSTSGGYYNSDFYEY
                     RIRFREFSEEDQNPWRIWNSSNDPTLKGTVFNPPAYPSDNPSYHFSNGMKYTVIPRLS
                     LFTRYEFYISAVDVFGNESPAPAEYFMMMTQPYSVEASVSDGRVKYDDFTNLNDPSLR
                     PVTESNIRVDLNIVSTDVSPDEVRVWYTAESDSLSIVGADNRVNEDEFPENSLYSVGA
                     VATNQNRWTAYMSSDSNAITDGSTVRFIVETITGGASTFSDADISDLDPNNNEWNFVV
                     RNLHTFTPQPVRILNNVLTDSNPLAYPSYYLTEDAYVTITVHDVKGRRVSTILEKAFR
                     RAGQNIKEKGWNGSNRAGRKLGVGLYYIKIVAKNKNGKTVLSEVKKVVVAK"
     gene            2012..2923
                     /locus_tag="P5123_11540"
     CDS             2012..2923
                     /locus_tag="P5123_11540"
                     /inference="COORDINATES: protein motif:HMM:NF024603.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ig-like domain-containing protein"
                     /protein_id="HRX16936.1"
                     /translation="MLTRSNKHIIFTVLLLLCSCDESKTVVYTPQQQTPGTVDQSTLL
                     PQIQYFYPGTLQTLDVPYPYPTDIPPDSTHMILVFSDIIENRDNQMNEWISLTRNGSA
                     ENFSITPNNSSYIFEIVPDNPVTFSANYSVFISSNAHKEGDDQATLNLSPLVQPPATT
                     INPPDSEKVEYTFETGITASSDFSPPSVLMTDPINGENNVEVNLTTSDGSIVIIFNDN
                     ANPMINPLTVDNTTITLTHVGFGPVSGSLSLASDDYNFKRYNFTPNSLPLVAGSTYLL
                     TISVGNAITDFSGNSVPETTSFFSTAP"
     gene            3350..>4753
                     /locus_tag="P5123_11545"
     CDS             3350..>4753
                     /locus_tag="P5123_11545"
                     /inference="COORDINATES: protein motif:HMM:NF012200.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="choice-of-anchor D domain-containing protein"
                     /protein_id="HRX16937.1"
                     /translation="MKKLFYIILTAIFFLACGKGEDSDITGGGASSDESFSNINITRN
                     SSTLNSGSSEINLGTVRTGEEGSSAEIKISNNGNINLSVTEIQITGADPNDFSTDFDS
                     ETEIAPDESETVTIYFSPATTTIGNRNAELRIRHDDENSENPYTILLTGSVTTTVSDI
                     WVIDNNSNLVKNGLSESNFGTINEGDTTAYRTFTIKNSGSANLNINSINISGSQFDLD
                     TSSTASSLAPDNSTTSFQVRFAPSTSGFFSETITINSNDPDEPNFSFTVSGTALDTGS
                     VMTPRIMLKVDNSGVTGGSTINFGSLRVNPANSPSSETKTITIYNVGSAPLNLSSINT
                     ITGDYTRTSPGSTTIATGGSTSFTITYNPDDILAPTAHTRTLIIANDDPLNSSFTLNL
                     TGLAYDPVMQTISSGTNMGNNASQQTANPTTSSEIVTISNTGNYDLVIQNITATGDTA
                     HFSDTRSGAIGTIAAGGN"
BASE COUNT         1505 a          986 c          821 g         1441 t
ORIGIN      
        1 tcgctgatgg tactgcatgg gtgaccgtgt gggagagtag gtcgctgccg ggattatata
       61 tattttttta cttcgccaca actacttttt taacttcact cagtaccgtt tttccatttt
      121 tattttttgc aactatcttt atataatata atccgactcc cagttttcgt cctgctctgt
      181 tggaaccgtt ccaccctttt tctttaatat tttgtcctgc tcttctaaaa gctttttcca
      241 gaattgtaga tactcttctt ccttttacat catgaaccgt tatggtgaca taggcgtctt
      301 ccgttaaata gtaggaaggg taagccaaag gattactgtc tgtaagtaca ttgttcaata
      361 ttctgacagg ttgaggagta aatgtgtgta aatttcgaac cacaaaattc cattcgttat
      421 tatttggatc aaggtcagaa atatcggcat ctgaaaatgt tgaagcccca ccggttatag
      481 tctcaacaat gaatcttaca gttgacccgt cagttattgc attactatct gaactcatat
      541 aggctgtcca tctgttctga tttgttgcga cagcgcctac tgaatataaa ctattttcag
      601 ggaattcgtc ttcgtttaca cggttatccg ctccgacaat gcttaaggaa tcactttcgg
      661 cagtatacca cactctgacc tcatcgggag agacatctgt cgaaacaata ttcagatcaa
      721 ctcttatatt ggattcagtt acgggtcgaa gcgatgggtc gtttaaattt gtaaaatcgt
      781 catatttaac tcttccatcc gagacagaag cctctacaga gtaaggttga gtcatcatca
      841 taaagtattc tgccggagca ggagactcat taccaaagac atcaacagcc gaaatgtaaa
      901 actcatatct ggtaaacaga gatagacggg gaattacagt atacttcata ccattcgaaa
      961 aatggtatga aggattgtca gaaggataag ctggagggtt aaaaactgtt cctttcaatg
     1021 ttgggtcgtt cgatgagttc caaattctcc agggattctg atcttcttca ctaaattctc
     1081 tgaaacgtat tcgatattca taaaaatcag aattataata ccccccggat gtggaagtta
     1141 ctaatggcga ccacattatt cttgcactgt ttgttgttaa actgacactt atttcggttt
     1201 catcagccgg aggaaacaca ctgctctgag gaggatcatt ccggtcccaa tagatggtct
     1261 gtgacactga tgagataaga ttatcggcat gctggcccga agctgcagtc acggtatact
     1321 cgaatggaat tgtttgacca ttggttataa atgaatcagg gaaaccggaa gcatcagaaa
     1381 atggcacaag cgcgatcacc cggttttgcc ccgaaggtat tgtcacatca aaacttgctg
     1441 tattcgagcc atttgaaaaa gatactgtaa acgctaccgg gccgtacata tgcgtccagc
     1501 ctgaaacatt catctgtgct gtaagctcta tcaatgtacc tgactgacca taagacctgt
     1561 aataatttga cgaattatac aacccactat gagtagggcc gacacctgtt ccataaagaa
     1621 aacgaattcc gctaactctt acatcatact ccgggccggg aataccggca acagtaaacg
     1681 taaacaatag ggacaaaaac gcggaaaata atttgttttt atgtttaaaa ttcgctgata
     1741 tcatcatttt gctattgtac gcctctaaaa caattatata gaaatccgat aaatgagtat
     1801 accttaaaac actctataaa aatattgtag aaaactttga aaaacgctac aagtttcgct
     1861 taatatttat tattttcaca agtttcttgt taaaatgtta aataatagag aatcctgata
     1921 ttatgattgc ttactacaac tattttatta ttatatcata catattattt tatcgggaat
     1981 tattcaaatt atatagagac aaagaaagat aatgttaact agatcaaata aacatattat
     2041 atttactgtt ttgcttcttc tatgctcctg tgatgaatca aaaacagttg tctatactcc
     2101 gcagcagcaa acacccggaa cggttgatca gagcacactt ttgccacaaa tacagtactt
     2161 ttatccagga acactacaga ctcttgacgt cccctatccc tatcctactg acataccacc
     2221 agattctaca catatgattc tggtatttag cgatatcata gaaaatcgcg ataatcaaat
     2281 gaacgagtgg atatcactta cacgtaacgg ttctgctgaa aatttttcca ttactccaaa
     2341 taactcatcc tatatttttg agattgtacc agataaccct gttacatttt cagccaatta
     2401 ttctgttttt atctcttcaa atgcccacaa agaaggcgat gatcaagcta cgcttaattt
     2461 atcacctctt gtgcaaccac cagcaacaac gattaatcca cctgacagcg aaaaagttga
     2521 atacacattc gaaacaggga ttactgcttc atccgacttt tctccgccat ctgttttaat
     2581 gaccgatcca ataaacggcg aaaacaacgt agaagttaat ctaactacat ctgatggttc
     2641 aatagtgatt attttcaatg acaatgccaa ccccatgata aatccactca cagttgacaa
     2701 cactacaatt acactaaccc atgtgggttt tggacctgtt tcaggatctc taagtcttgc
     2761 ttctgatgat tataatttta aacgctacaa ttttacaccc aattcattac ctcttgtcgc
     2821 aggatcaact tatcttctca caatatccgt aggtaatgca attacagatt tttcgggcaa
     2881 cagtgtcccg gaaacgacta gtttcttttc aactgcacct tgatagttcc cacttcttca
     2941 gttgatatac acggattcac agttttcttt tagttttact caccaagatt ttttgttttg
     3001 atagacaatt ttatggaaat aacgattttc aaaaaaatta cagtactgtt cgaattttca
     3061 tttcagagga attcattata catatttacc agtaatatac cgatatttat atatagtatt
     3121 ttttttaata catccattct gcatttgaat atggcagtaa ataattaatt atcaataaaa
     3181 aaaggattgt aactgtcctg tttttatagt aatttataaa atgggtcttt ttgcttatac
     3241 agtaattata caatacgata ttattttgaa aaataacaac tattgaaaac tcgagttggt
     3301 ctttttaagg tttaaaccgg aagaaatcta aggttatggc ttttagggaa tgaaaaaact
     3361 attttatatt atccttactg ctattttttt tctagcctgc ggaaagggcg aagactctga
     3421 tattactgga ggaggcgcga gtagtgatga aagcttttcc aacattaata ttacccgcaa
     3481 ttcaagtact ttaaattcag gaagttccga aatcaatctt ggaaccgttc gcactggtga
     3541 agaaggaagc agtgccgaaa tcaagatatc aaataatgga aatattaacc tttctgtaac
     3601 tgagattcaa attacaggtg ccgaccccaa tgatttctca acagattttg attctgaaac
     3661 ggaaatagct ccagatgaat ctgagacagt aacaatttat ttctctcctg caacgaccac
     3721 aataggaaac agaaacgccg aattacgtat tcgacatgat gatgaaaact ctgagaaccc
     3781 atatactatt ttgcttacag gttcagtaac aactactgta tctgacattt gggtaataga
     3841 taataacagc aatttggtta aaaacgggct atcagaatca aattttggaa caataaacga
     3901 aggcgataca accgcgtaca gaacatttac tattaaaaac agtggttccg ctaatcttaa
     3961 cattaattca atcaacataa gtggctcaca gtttgacctc gatacttcat caactgcttc
     4021 atcactggct cctgataaca gtaccacaag tttccaagta cgatttgccc caagcacttc
     4081 cggtttcttt agcgaaacta taaccataaa cagcaatgat ccagatgaac ctaatttttc
     4141 ttttactgtg agcggcactg ctcttgatac cggttctgta atgactccgc gcataatgtt
     4201 aaaagttgac aatagcggtg ttaccggtgg ctctacaata aattttggct cattacgggt
     4261 taatccagca aacagcccaa gtagcgaaac aaagacgata actatctaca atgtgggcag
     4321 cgcgccactt aatctgagct ctattaatac cataactggt gactatacac gtacaagccc
     4381 gggttccact actatcgcaa ccggtggctc aaccagcttc acaatcacct ataaccctga
     4441 tgatattttg gcaccaactg ctcatacacg gactcttatt atcgcaaacg atgatccgct
     4501 taacagcagt tttaccctta atttgacagg actcgcctat gatccggtga tgcagacaat
     4561 aagcagcgga acaaatatgg gtaataatgc ttcgcaacaa accgcaaacc cgactacaag
     4621 cagcgaaatc gtgaccatat caaacacggg aaattacgat cttgtaatac aaaatatcac
     4681 agctaccggc gacacagccc attttagcga tacaagaagc ggggctatcg ggacaatcgc
     4741 tgccggtgga aac
//
LOCUS       DAOTGZ010000213         4706 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_3064
            NODE_21622_length_4706_cov_4.620942, whole genome shotgun sequence.
ACCESSION   DAOTGZ010000213 DAOTGZ010000000
VERSION     DAOTGZ010000213.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868088
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 4706)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4706)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:22:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,785
            CDSs (total)                      :: 2,751
            Genes (coding)                    :: 2,732
            CDSs (with protein)               :: 2,732
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 12 of 19
            Pseudo Genes (internal stop)      :: 5 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4706
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_21622_length_4706_cov_4.620942"
                     /isolate="AMR_MDS_3064"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..266
                     /locus_tag="P5123_11550"
     CDS             <1..266
                     /locus_tag="P5123_11550"
                     /inference="COORDINATES: protein motif:HMM:NF013468.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003725 - double-stranded RNA binding
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="Sua5/YciO/YrdC/YwlC family protein"
                     /protein_id="HRX16938.1"
                     /translation="KTIGIRIPDNNFLRELISVFNKPVISTSVDTEPGDYMIDPVFLE
                     EKYRKSVDLVLDAGLKNSELSTMIDFSGTSPVILRQGKGIVDI"
     gene            285..605
                     /locus_tag="P5123_11555"
     CDS             285..605
                     /locus_tag="P5123_11555"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRX16939.1"
                     /translation="MDKLTELMTNLVPFAILLCIGAAVSALFYYVKKRDLFGGFAGGM
                     VIAIAGALLGIFLDKFLLEYIIIVLKFLVYQFNVDIIAGFIGAYGAVYMMNKLNHDKK
                     RDRY"
     gene            complement(787..2436)
                     /locus_tag="P5123_11560"
     CDS             complement(787..2436)
                     /locus_tag="P5123_11560"
                     /inference="COORDINATES: protein motif:HMM:NF015983.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HRX16940.1"
                     /translation="MSMAKRYLILDFYVDEPACFGVPPFVSPYPRYIYGALIDAGVNS
                     DMIDYLTVDNLRLTGFKIYGFYKAVFIIGGAVVPGKYLGQKIGDTAEIKKTLLNNTDL
                     FFAAGGLAALQIAGSHNNLQSVNGDIEQYAHDFVCSVPDPSKLRLYSDLDRWAIAGAG
                     LVKRHPHFPNVICEIETYRGCPRSSHCSFCSEHIFKNIVYREQQSILAEIDALIETDV
                     SRFRIGRQADILAYGSRLDYFIKTFPRPCVQEVVSLFKELEKRKNEGKIEVLNVDNAN
                     PGTICHFEQESFDILQAIASAVTPGDTLPLGVESLDPVVIAKNNLKVDAETLIRVVKL
                     MNSAGGWREEGIPKLLPGVNLIHGLPGETIHSFRYNYETLMDILSQGLLLRRINIRSL
                     VPFPTTGMVYNASPKVINRYRYYRQLIRDNVDAVMLRGVFPSGSLLRSVRAEQVQNGV
                     TYCRQIASYAITVKIKEEISTGTVFTAAVCGHEPRSLRAFRNPLDINSASTRLLESVE
                     GIGKKRAGTIVLNRPYENSDQYGDSFSSVPELLKKRIIFKK"
     gene            complement(2433..2996)
                     /locus_tag="P5123_11565"
     CDS             complement(2433..2996)
                     /locus_tag="P5123_11565"
                     /inference="COORDINATES: protein motif:HMM:NF014069.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HDIG domain-containing protein"
                     /protein_id="HRX16941.1"
                     /translation="MDNSHIETAISIPSEEECFELFERYGMLKNIVRHSLRVRDVANA
                     LCSSLINPDSLNCSVVNAASLLHDIAKSITLKTGEHHDILGAKMLMQIGYPEIAAVVS
                     EHVTLKNFSIDTPLREAEIVFYADKRVLHDKLVTVDERISDLFTRYGKTAVSRQHILS
                     TQRILKELETKITHNLICTIQEALLTL"
     gene            complement(3017..3493)
                     /locus_tag="P5123_11570"
     CDS             complement(3017..3493)
                     /locus_tag="P5123_11570"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HRX16942.1"
                     /translation="MWALQGGLLHYFGAKEDLYFELVQQLCDDITAGRRSVLQGIADS
                     NDQASLSALYVIEQFFLDKNNIHVLLNLLLLSFEDESVREVFKKFFHENMGFFQTLIE
                     KKYQDVPQRRKSDLPAADLARILQIIVLAAGIVELVDPVNFDHNHLTTYILSILKG"
     gene            complement(3652..4566)
                     /locus_tag="P5123_11575"
     CDS             complement(3652..4566)
                     /locus_tag="P5123_11575"
                     /inference="COORDINATES: protein motif:HMM:NF012515.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PfkB family carbohydrate kinase"
                     /protein_id="HRX16943.1"
                     /translation="MIYGVGESLVDIIFKNNSAVGSTPGGSVLNSTVSAARLGASASL
                     VTDLGRDTAGQLVMSFLKDNGVSFNHAFIHDEDQTTIALAFLNESGDASYQFYKISSL
                     PSFNVNFNAADVLLAASFYAISPRTSEAVNSLVDDALRASAFVFYDPNYRPGNKEISL
                     EDSRERVIDLINKASAVRLSEEDSEYIFSTKDCSQIVNMIPSLSQKPLILTRAANGVD
                     LYYRGMLRSFDSVRIDPVSTIGAGDTFNAGIVAAVSAINPDARSQLDIEFYGKIIEAA
                     IKLSASVCLSMDNYIQKGDYTSFWNFID"
BASE COUNT         1491 a          972 c          863 g         1380 t
ORIGIN      
        1 agaaaacaat cgggatacgc atacctgaca acaatttttt acgagaactt atttctgtat
       61 ttaataaacc tgttatttca acatcagttg acacagaacc aggtgactat atgattgatc
      121 ctgtttttct agaagaaaaa taccggaaat cagttgatct ggtattggat gctggtttaa
      181 aaaattctga attatcgaca atgatcgact tttcaggaac atcaccggtt attttacgac
      241 agggaaaggg aatcgtagac atttagaaac attggagtgt aaatatggat aaattgactg
      301 aacttatgac caatcttgtg ccattcgcta ttttactatg catcggcgca gcagtttcag
      361 ctctgtttta ttatgtaaaa aaacgtgacc tgtttggtgg attcgcgggg ggcatggtta
      421 ttgctatagc aggagcacta cttggcatat ttctggacaa atttttactt gagtacataa
      481 tcatcgtact taaatttctt gtttatcagt ttaatgtcga catcatagca ggttttattg
      541 gtgcttatgg tgcggtttat atgatgaaca aacttaatca cgacaaaaag cgcgaccgtt
      601 attaatttca tctagttaaa ccgttcaaac gagccaagat ataaattctt tctttgaacg
      661 gtttgataca gattcagctt tttgattttg aaaaaaaatt cccaaaattt gttacggtta
      721 attataaaga cactacttat ttacaggata caccggaact tcaagttaca aatagaatac
      781 aatatcttat tttttgaaaa taattctctt ctttaacagt tccggtacag aagaaaagct
      841 atcaccgtat tgatcggaat tctcatatgg cctgttaagc actattgtac cggctcgctt
      901 ttttcctatg ccctcaacgg attcaagaag tctggtagat gcactattaa tatcaagtgg
      961 attccgaaat gctctcaacg atcgcggctc atgaccacat acagccgctg taaatactgt
     1021 accagtcgaa atctcttctt tgattttgac tgtaatggca tatgaagcaa tctgcctgca
     1081 atatgtcact ccattttgaa cctgttccgc tctaaccgac cgcaaaagtg atcccgaagg
     1141 aaaaacccct cgaagcatta cagcatcaac attatctcgg attaactgcc tgtaataacg
     1201 gtaacggtta atcaccttgg gcgaagcgtt atataccata cctgttgttg gaaacggcac
     1261 aagagacctt atgtttatcc gacgcagaag aaggccttgt gacaatatat ccatcaatgt
     1321 ctcatagtta tagcgaaaag aatgaatcgt ctcgccaggc aggccatgta ttaagtttac
     1381 tcctggcaat aacttcggaa taccctcttc gcgccatccc ccggcactat tcattaattt
     1441 cactacacga ataagggttt cggcatctac ctttaagttg ttctttgcaa ttacgactgg
     1501 gtcaagagat tcgacaccaa gcggcaaggt atcaccggga gtaacagcag aagctatcgc
     1561 ctgaagaata tcaaatgact cttgttcaaa atggcaaatg gttcccggat ttgcattatc
     1621 aacattaagt acctcaattt tgccctcatt ttttcgtttt tcaagctctt taaaaagaga
     1681 aactacctct tgtacacatg gacgtgggaa ggttttaata aaataatcca aacgagatcc
     1741 ataagccaaa atatcagcct gacgacctat cctaaatcgc gacacatctg tttctatcag
     1801 agcatctatt tctgcaagta tactttgctg ttctcggtaa actatgtttt taaaaatatg
     1861 ctccgagcaa aaactgcaat gcgatgaacg agggcaccca cgataagttt caatttcaca
     1921 gataacattc gggaaatgcg ggtgcctttt tacaagacca gcacccgcaa ttgcccaacg
     1981 atcaagatca ctgtaaagac gcaattttga ggggtcgggc accgaacata caaaatcatg
     2041 agcgtactgc tcgatatctc cattaacgct ttgtaaatta ttatgactac cagctatttg
     2101 aagtgccgct aaaccgcctg cggcaaaaaa aagatcggta ttattcaata gcgtcttttt
     2161 aatttcggcc gtgtctccta ttttttgacc aagatactta ccaggaacaa cggccccacc
     2221 gattataaag acggctttat agaagccgta tattttaaaa cctgttaatc gcaaattatc
     2281 gactgttaaa taatcaatca tatcactgtt tacaccggca tctattaacg caccgtatat
     2341 gtaacgcgga tatggagaaa caaatggggg tactccaaaa caggcgggct catccacata
     2401 aaaatcaagt atcaaataac gtttagccat gctcataatg tcaaaagagc ctcctgaata
     2461 gtgcaaatta aattgtgtgt aatttttgtt tccagctctt ttagtattct ttgagtagac
     2521 aatatatgct gtcttgaaac cgcagtttta ccataacgtg taaaaagatc acttatccgt
     2581 tcatctacag ttacaagctt atcatgcaac actcttttat ccgcgtaaaa aacaatttcg
     2641 gcttcccgca gaggggtatc gattgaaaaa ttttttagag taacatgttc ggatacaact
     2701 gctgctattt caggataacc aatctgcatt agcatttttg caccaagtat atcatgatgt
     2761 tcacctgttt taagggtgat tgatttggcg atatcatgca aaagcgacgc tgcgttaaca
     2821 acactacaat taagagagtc gggattaatt aacgaagaac aaagagcatt agctacatct
     2881 cgcactcgca aggaatggcg aacaatattt ttgagcatac cataacgttc gaaaagctca
     2941 aaacactcct cttcacttgg tatacttatt gcagtttcga tatgtgaatt gtccataaaa
     3001 gccagatttg atctaactat cctttaagta tggagagtat atatgtagta agatgattat
     3061 gatcaaaatt cacaggatcc acaagttcta caatacctgc agccaaaaca attatctgca
     3121 aaatacgcgc caagtcggcg gcaggaagat cagacttacg acgttgaggg acatcctgat
     3181 attttttttc gattaaggtc tggaaaaaac ccatgttttc atgaaagaat ttcttaaaga
     3241 cctcacgcac actctcgtct tcgaaactaa ggagaagaag attaagaaga acatggatat
     3301 tgtttttatc aagaaaaaac tgctctataa catagagagc cgaaagacta gcctgatcgt
     3361 tagagtccgc aatcccctgt aacactgaac ggcgaccggc tgtaatatca tcacaaagtt
     3421 gttgaaccag ttcaaaatag agatcttctt ttgctccgaa ataatggagc agcccccctt
     3481 ggagagccca cattcatcgg ctacattctg gatcgtaatg tttgaataga catccctctc
     3541 gagacagcta cgaaaagcat taataatctc gtgttttttc tcttttatct tcttttcttt
     3601 gtccatgata ccgtataatc agtttgtaac gacaataatt gcaaggattt tttaatcaat
     3661 aaagttccaa aatgatgtgt agtcaccttt ttgaatatag ttatccatac ttaaacatac
     3721 tgacgccgaa agtttaatgg cagcttcaat aattttgccg taaaattcaa tatctaattg
     3781 ggatcttgca tccgggttta tggcagaaac tgcggcgaca ataccggcat tgaaagtgtc
     3841 acccgcacct atcgtactta cgggatcgat tctaacagaa tcgaacgagc gcagcatgcc
     3901 tctgtaatat agatccacac cattagccgc acgagttaaa attaatggtt tttgcgacaa
     3961 cgaggggatc atgtttacaa tctgagaaca atcttttgtc gaaaatatat actcagaatc
     4021 ttcttcagaa agtcttactg ctgatgcctt attaatgaga tcaataacac gctcacgact
     4081 atcttccaaa cttatttcct tatttccagg gcgataattt ggatcataaa agacaaatgc
     4141 cgaagcacgc aaagcatcat caacaagact gttaacagct tctgacgtgc ggggagatat
     4201 agcataaaat gaggcagcaa gaagtacatc tgccgcgttg aaattgacat taaatgaagg
     4261 aagagaagat attttataaa actgatagga agcgtcaccc gactcgttaa gaaaagctaa
     4321 tgcaattgtc gtttgatcct catcgtgaat aaacgcgtga ttaaacgaca ccccattatc
     4381 tttaagaaaa gacattacta actgccctgc agtatctcga cccaagtcgg taacaagaga
     4441 tgccgaagct cctaatctgg cagcgctaac tgtcgagtta agaaccgagc caccgggggt
     4501 agaaccaacg gccgaattat ttttaaaaat tatatccact aaagattcgc ctactccgta
     4561 aatcatccaa ccctcttttt ttattttttt ttcgattttg attgacaagt atatgcccaa
     4621 agtatttatt gtcaatacat tggttatgaa attttcccgg tggctataga gagagggtca
     4681 cacctgttcc cattccgaac acagaa
//
LOCUS       DAOTGZ010000214         4652 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_3064
            NODE_21942_length_4652_cov_4.252338, whole genome shotgun sequence.
ACCESSION   DAOTGZ010000214 DAOTGZ010000000
VERSION     DAOTGZ010000214.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868088
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 4652)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4652)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:22:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,785
            CDSs (total)                      :: 2,751
            Genes (coding)                    :: 2,732
            CDSs (with protein)               :: 2,732
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 12 of 19
            Pseudo Genes (internal stop)      :: 5 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4652
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_21942_length_4652_cov_4.252338"
                     /isolate="AMR_MDS_3064"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1990
                     /locus_tag="P5123_11580"
     CDS             <1..1990
                     /locus_tag="P5123_11580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013488923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="glycosyl transferase"
                     /protein_id="HRX16944.1"
                     /translation="ITNTSASPKTLDFVPVVEYTHFEALKQFTNADWVPQTMQSKMDE
                     IDGKTVLIQYPFMRRDTKWNYFTSNHSVSSFETDRAKFLGSNGYGSWLAPESLQQKDL
                     LKSEALRGDNIAALLHELGELAPGESRRIILQLGQTDKINDMRSVISEYESKESVDKA
                     LEELSLFWDEYLSAFEVDTPSEAMNNMLNVHHPKQCFITKNWSRYLSSYQLGLGARGL
                     GMRDSSQDLLGVMSHIPEEASLLMRDLLAIHRPDGSAMHQFYPSTKEANEGDSREMPD
                     RPDYYGDDHLWILISLATYIRETGNIAILEEEIPFYTKKNLPKESGTVRDHIDRAINF
                     TRNNTGKHGLPLLGFADWNDTVNLPTGSESIFNASLYGVALKEMIDLFDYLGDKETSS
                     RYQKYWQEMKDVVNNEAWDGEWFVRYFDQNGSPIGSNKNDKGKIYVNGQSWPVMAGFA
                     REEYGNKALESVYTKLNTENGIKVSWPGYDGFDAEKGGITTYPPGAKENGGIFLHTNP
                     WVMIAETMRGNGNRAFQYYQQINPAVKDIDIYELEPYCYAQNILSDEHPQSGLARNSW
                     LSGTAAWTYRAATWYILGIRPGYNGLIVDPCIPSEWDGFKVTRRLRGAVYNIIVKNPS
                     HIQKGVKSCTVDGVSVSNTAIPYIPKGESCTIEVTMNL"
     gene            complement(2052..3230)
                     /locus_tag="P5123_11585"
     CDS             complement(2052..3230)
                     /locus_tag="P5123_11585"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRX16945.1"
                     /translation="MRNRTIYILLTIMLASTSSTLIADQPVISGISESSVKAGDTCYT
                     GYTWMAEQFANIRMQADLNDKGAIHAAVNAGVSSDKEKKTTTNIEIERLYMSIYGEKS
                     DISTGFMRIPFGYGQAFKPSDFINPQNPLYPEARLKGVLGASAQFYHIQDFKIQLFAA
                     DRSDSAHYMEERSILAGGPSLDYHNSLFSIQTLYAIQQPIDRSSNSPVHFAGLSLKFD
                     ATAGFAIDTLYAHDGEDSGEPDGLKAAFGVDYSLLKGKLYLLMQYFYNGTSSLKSSDK
                     LDKLYGSDDWYKLPPYDRIPVNGFHEYYRRHYLLLNGVYNLSDYTSISAIIVANIEDY
                     SLIPSIRIEHQPFQGLTLGLTFRKPIDPYIINDGSKGELGPHHTGYKNQLLIDASMRF
                     "
     gene            complement(3239..3985)
                     /locus_tag="P5123_11590"
     CDS             complement(3239..3985)
                     /locus_tag="P5123_11590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013969632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_process: GO:0042953 - lipoprotein transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane lipoprotein-sorting protein"
                     /protein_id="HRX16946.1"
                     /translation="MKKTKASLILSLALFMSSIIAQDSPDAKAILDHIDKNERFNTIE
                     YTGEFTVEYKGKTFVKKFKAYGRGNEDSFIEFTNSEDRGTKYLKKDGRLYVYSPDTEQ
                     VMLISGHMLKESMMGSDMSYEDTIENEKLSKRYTAVVSGSETIEGQECYVLDLKAIKK
                     TESYPKQKLWVAKDNKMLIKYELFALSGAKLKEYKLIRSEKIAGKNFPVESQLKDLLR
                     KGSRTVFKLDNVVIDKPISASIFSMRNLER"
     gene            complement(4048..>4652)
                     /locus_tag="P5123_11595"
     CDS             complement(4048..>4652)
                     /locus_tag="P5123_11595"
                     /inference="COORDINATES: protein motif:HMM:NF014717.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="FtsX-like permease family protein"
                     /protein_id="HRX16947.1"
                     /translation="RLQKILGLNDATQQLVIFADKTSYSGTIRNKLTEILSDNSHITE
                     WNENYWVAMFQSMTGIYIIIFAVFQIVAGFLIINTMFMIVHERIKEIGMMGALGFKRS
                     EIVTVFFLEALLLSMLGAFAGAVIGGLGSWIWSLYPIDMNSITGGGMKDFPMSGTFYL
                     YFSVSTIIEGFLFGVIITGLCTLVPSLKSAFIQPVEALRR"
BASE COUNT         1351 a          882 c          971 g         1448 t
ORIGIN      
        1 aataacaaac acttctgctt cgcctaaaac tctggatttt gttcctgttg ttgaatatac
       61 gcattttgaa gctttgaagc agtttactaa tgctgactgg gttccccaaa ccatgcaaag
      121 caaaatggat gaaatagacg gtaaaacagt tcttatccaa tatccgttta tgcgtcgaga
      181 tacgaaatgg aattacttta catcaaatca ttctgtgtct agttttgaaa ccgatcgcgc
      241 aaagtttctt ggaagcaatg ggtacggttc ctggcttgca cctgaatcgc tgcaacaaaa
      301 agatcttttg aagtcggagg ctttaagggg tgataatata gcagctcttt tacacgaact
      361 tggggaactt gcgccaggtg agagtcgcag gatcatattg caacttggtc agacggacaa
      421 gattaatgat atgcgatcag ttataagtga atatgagagc aaagagtctg tcgacaaggc
      481 gttagaagaa ctctcattat tctgggatga atatctttcg gcttttgaag tggatactcc
      541 ttcagaggct atgaacaata tgcttaatgt acatcatcca aaacagtgct ttattacaaa
      601 aaactggtca cgttacctat cgtcatatca gttgggtctt ggagcgagag ggttgggcat
      661 gagggactct tcacaggatc tattaggggt aatgtctcat ataccagaag aagcctcgct
      721 ccttatgcgt gatcttttag ctatacatag accagatggt tcggccatgc atcagtttta
      781 tccttcaact aaagaggcta atgagggtga ttcgcgtgaa atgcccgaca ggccggatta
      841 ttacggagat gatcatctct ggatattaat ttcacttgcc acatatatcc gtgagacggg
      901 taacattgca atactggaag aagaaattcc tttctatacc aaaaaaaacc taccaaaaga
      961 gagcggaact gttcgtgatc atattgatag agccataaac tttacacgta ataataccgg
     1021 aaaacatggg cttcctcttc ttggttttgc cgattggaat gatactgtaa atttaccaac
     1081 aggttcggaa tctattttta atgcgtcact ttatggtgtt gctttaaaag agatgataga
     1141 cctttttgat tatcttggtg ataaagaaac aagtagtcgt tatcaaaaat attggcaaga
     1201 gatgaaagat gttgttaata atgaggcatg ggacggagag tggttcgtaa gatattttga
     1261 tcaaaatggc agccctattg gatcaaacaa aaatgataag ggtaaaatat atgttaacgg
     1321 acaatcgtgg cctgtaatgg cgggatttgc cagagaagaa tatggcaaca aggctttgga
     1381 gtctgtatac acaaagctta acacggaaaa cggcatcaag gttagttggc ctggttatga
     1441 tggttttgat gcagagaagg ggggaataac aacatatcct cctggggcaa aagagaatgg
     1501 cggaatcttt ttacatacga atccctgggt tatgattgct gaaacaatgc gtggaaacgg
     1561 aaaccgtgct tttcaatact atcagcagat taaccctgca gttaaggata ttgatattta
     1621 tgaattggaa ccctattgtt atgcacaaaa tattctgagt gacgagcatc cacaatccgg
     1681 acttgcgcga aacagctggc tttccggaac cgccgcgtgg acttatagag cggcgacctg
     1741 gtacatattg ggaattcgtc ccggatacaa tgggttgatt gtagatcctt gtattccgtc
     1801 tgaatgggat ggattcaagg tgacaagaag attgcgcggc gctgtatata atattattgt
     1861 gaaaaaccct agtcatattc agaaaggtgt gaagagctgc actgttgacg gtgtttctgt
     1921 cagcaataca gccattccgt atatacctaa aggtgagagt tgtaccattg aggttacaat
     1981 gaatctttaa ctcctttaaa atactttgcc tttaagctga ccgcctgcca attttcggca
     2041 ggcgtttttt tttagaaacg catggaagca tcaattagta gctgattttt atatccggtg
     2101 tgatgtggac caagttcacc ttttgatcca tcattaataa tataaggatc aattggtttt
     2161 ctaaaggtta gcccaagtgt tagtccctga aatggttggt gttcgattct gattgaaggt
     2221 attaaagagt aatcttcaat attagcgact attatggcgc ttatacttgt gtagtccgat
     2281 aaattgtaca cgccgtttaa taaaagatag tggcgcctgt agtattcatg aaaaccattt
     2341 accggtatac gatcataagg tggtagttta taccagtcgt ctgatccgta aagtttatca
     2401 agtttgtcac tgcttttaag cgatgatgtt ccattataaa aatattgcat aagcaggtac
     2461 agcttgcctt taagaaggga ataatcaaca ccaaatgcag ccttaagacc atcaggttca
     2521 ccactgtctt ctccgtcatg cgcgtacagc gtgtcaatgg cgaatccggc ggttgcgtca
     2581 aattttaatg atagtcctgc gaagtgaaca gggctattag agctgcgatc tattggttgc
     2641 tgaatggcat agagtgtttg tatgctaaaa agagaattat ggtaatcaag actaggtcca
     2701 cctgcgagaa ttgaacgttc ttccatgtag tgagcagaat cagatctatc ggctgcgaaa
     2761 agctgaattt taaaatcttg tatatggtaa aattgagcac tagctcctaa tacacctttt
     2821 aaacgcgctt ccggatagag cgggttttgg ggattgataa aatcgctggg cttaaacgcc
     2881 tgaccataac cgaacggaat tctcataaag ccggtgctga tatcgctttt ttcaccatat
     2941 attgacatat atagacgctc aatttcgata ttggttgtgg tctttttctc tttatctgaa
     3001 ctgactccgg catttaccgc agcatgaatt gctcctttgt cgttcaagtc agcttgcatt
     3061 cgaatatttg caaattgttc tgccatccat gtataacctg tataacaggt atcaccagcc
     3121 ttaacagagg attccgagat cccggatatt acaggctgat ccgcaattag agtggatgac
     3181 gtacttgcca gcattattgt caacaggatg tagatggttc tgtttctcat gaaaaatttc
     3241 accgttcaag attacgcatg ctgaaaattg aggcggatat gggcttgtcg attactacgt
     3301 tatcaagttt aaaaacagta cggctccctt tccttaatag atcttttagt tgactctcta
     3361 ctgggaaatt tttaccggcg atcttttctg atctgattaa tttatactcc ttaagtttgg
     3421 cgccagataa agcaaagagt tcgtatttaa taagcatttt gttgtcttta gcgacccaga
     3481 gtttttgctt tggataactc tcggtttttt ttatggcttt aaggtcaagt acatagcatt
     3541 cttgcccctc aatggtttca cttccagaga ctacagctgt atatcgtttg gaaagttttt
     3601 cgttctcgat tgtgtcttcg tagctcatat ccgatcccat catcgactct ttaagcatgt
     3661 gccccgatat aagcatcacc tgttctgtat ccggtgaata cacatataac cggccatctt
     3721 ttttcagata tttagtacct cgatcttcgc tgttggtaaa ctcgataaag ctatcttcgt
     3781 tacctcgtcc ataggcttta aattttttca caaaggtttt acccttatat tcgacggtaa
     3841 attcgcctgt atactctatt gtattaaatc tttcgttctt atcgatatgg tcaagaatgg
     3901 ccttagcatc tggtgagtcc tgtgcgatta ttgaactcat aaaaagtgct aatgataaaa
     3961 tcagtgatgc ttttgtcttt ttcattcttt tctcctttat gtttattgta gaaaattatt
     4021 tgttctgtaa aattttttaa ttattaatta tctccttaag gcttctaccg gttgaataaa
     4081 agcactttta agggatggta ctagagtaca aagccctgta attataactc cgaaaagaaa
     4141 cccttcaatt attgtagaaa ccgaaaaata cagataaaat gttcccgaca tgggaaaatc
     4201 cttcatgccc ccaccggtta ttgaattcat gtcaataggg tagagcgacc atatccagga
     4261 accaagacca ccaataactg ctccggcgaa tgctccaagc atacttagaa gcaatgcttc
     4321 taaaaaaaac actgttacta tttccgatct tttaaagccc aatgccccca tcatgccgat
     4381 ctcttttatt cgttcgtgta caatcataaa cattgtgtta ataatcaaga atcccgctac
     4441 aatttgaaaa accgcgaaaa tgataatata tattccagtc atcgattgaa acattgccac
     4501 ccagtaattt tcattccact cggttatatg agaattatct gaaagtattt cggtcagctt
     4561 attcctgatt gtgcctgagt atgacgtttt gtctgcgaag atgacaagct gctgggtagc
     4621 atcgtttagc ccaagaattt tttgaaggcg at
//
LOCUS       DAOTGZ010000215         4615 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_3064
            NODE_22177_length_4615_cov_4.409430, whole genome shotgun sequence.
ACCESSION   DAOTGZ010000215 DAOTGZ010000000
VERSION     DAOTGZ010000215.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868088
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 4615)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4615)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:22:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,785
            CDSs (total)                      :: 2,751
            Genes (coding)                    :: 2,732
            CDSs (with protein)               :: 2,732
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 12 of 19
            Pseudo Genes (internal stop)      :: 5 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4615
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_22177_length_4615_cov_4.409430"
                     /isolate="AMR_MDS_3064"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1066)
                     /locus_tag="P5123_11600"
     CDS             complement(<1..1066)
                     /locus_tag="P5123_11600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013008244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="potassium transporter TrkG"
                     /protein_id="HRX16948.1"
                     /translation="MKPENIFKPLCALLIIIAINMIVPALIAIYYHEFNSLRAFCYVI
                     ASIIILCSTILFFSRKTKESQLQTREGFLFVTLSWISASAMGALPLYISGSIPDFTSA
                     FFEIISGFTTTGATILTDIESLPRSILFWRSQTHWLGGMGIVVLTVAVLPMLGIGGLQ
                     LIKAEAPGPKMDKLAPRVTGTAKILWIIYLGLTVIETFLLMFGGLNLFDALTTTFGTL
                     ATGGFSTYNSSIGYFDSFYVHTVVTVFMVLAGINFSLHYRLITGNIMQILKDSEYKLF
                     ISIFTVSSILIAVNLFYKGLYHSFYDSLRHAAFQTASILTTTGFASTDYSNWPSISLS
                     ILFILMFIGGCSGSTGGGIKI"
     gene            complement(1056..2408)
                     /gene="trkA"
                     /locus_tag="P5123_11605"
     CDS             complement(1056..2408)
                     /gene="trkA"
                     /locus_tag="P5123_11605"
                     /inference="COORDINATES: protein motif:HMM:NF007031.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015079 - potassium ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Trk system potassium transporter TrkA"
                     /protein_id="HRX16949.1"
                     /translation="MQIVIAGAGAVGTQIAKHLITEGKNVVLIESDPSRANYASSTLD
                     CMVIEGSASSPADLLKAGIIKADFFISVTDSDEINMVASGIVKTLFNVKKIIARIRNP
                     EYLSGSIISEEFLNIDYAVNPEAEAANELVEITEFGAHSHIISFKKSNVRLINMAIQN
                     NSVLVGKSLLSIRTSLDPDFFVAAILREDQLIIPSGNTCINENDNIYFMAPDHNIKGL
                     FKFSDYKKIKLRKVLIVGGTPLGTRVLSRLLNNNYNVTLIDKDYKNCKKLSEIYPEAL
                     IINADITDENIYKDELVHKYDLIICLTNNEELNLLSALYAKKSGIKRAIALIVNNNYL
                     NLSMQLGIDSVISPKSSSVDSILKYIRRGNVSSIKSIFNGRAEILEFSLDKKSPAIGI
                     MLKNINMPSSSLIISINKNNEKIVPTGNYIFTENDSLLIITTKESITQIEKIFTANEA
                     "
     gene            2488..3678
                     /gene="dinB"
                     /locus_tag="P5123_11610"
     CDS             2488..3678
                     /gene="dinB"
                     /locus_tag="P5123_11610"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: protein motif:HMM:NF002677.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003684 - damaged DNA binding [Evidence
                     IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase IV"
                     /protein_id="HRX16950.1"
                     /translation="MKRIIIHIDMDAFFAAVEQNDNPKLKGKPVVIGADPEGGKGRGV
                     VSTASYEARKYGIRSAMPVSEAYRRCPDAIFIRGNMYRYKEVSDTIMSVFRSFSPVVE
                     PLSLDEAFLDCSGSSKIFGSPVEIAQKIKNSIYQSTGLTSSAGVSAVKSVAKIASDLR
                     KPDGLTICQEGNEIEFLKELSISRLWGVGKKSYKILTDMGFYKIGDISSRTRKDMELI
                     LGRSGAELWDLSRGIDFRPVALENERRKSISEEITFKEDKKSFDELTHTLKYICDSLS
                     FSLCKEACKARTITLKLRYDSFETITRGKTVAECTRSYNVIFNTVLELMGSCLVIGRR
                     VRLIGVSLSNLEYGKYEAQPDLFSLKESLIESPLDNVINDLKIKYGSKIKRASLLRHG
                     RDNE"
     gene            3671..4474
                     /locus_tag="P5123_11615"
     CDS             3671..4474
                     /locus_tag="P5123_11615"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF021613.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3'-5' exonuclease"
                     /protein_id="HRX16951.1"
                     /translation="MSKENKYIVFDIESVPDGKLIKDVKYPGEDISEDEAISRLQDEI
                     MDKTAGKSNFIPVTMQYPICVVVALIAEDFTVNEIKSLDYGEYRASEIARSFWFRVEV
                     LDKKASLVTFNGRGFDIPIMELMAYRYGHDMKRHLTDKFGTRFRFGTRHIDLQDFIGN
                     YNAIGVFGGLNMLAKTIGLPGKTDTAGDQVYDLYKEGKIEQINDYCIHDVLDTYLVFL
                     RTRVMLGEITLDDEKKIRENCFSYLKESCEYKNAAISYLSHIKDFEPWP"
BASE COUNT         1512 a          768 c          902 g         1433 t
ORIGIN      
        1 taattttaat accgcctcct gttgatccag agcaaccacc aataaacatt agtataaaaa
       61 gaattgataa ggatattgat ggccagtttg aataatctgt cgatgcaaaa cctgtagtag
      121 ttaatataga agccgtttgg aaagccgcat gtcttaagga gtcataaaag ctatgatata
      181 aacctttgta aaaaaggtta acagctatta atatgctcga gactgtaaat attgatataa
      241 acagtttata ttcagaatct ttaagtatct gcataatatt accggttatg agtctataat
      301 gcagagagaa atttatacca gccaatacca taaaaacagt gacgacagta tggacataaa
      361 atgaatcaaa gtaacctata gagctgttgt atgttgaaaa tccaccagtt gcgagagtgc
      421 caaacgttgt tgttaacgca tcaaaaagat ttaaccctcc aaacataagc aaaaaagttt
      481 ctatgactgt taatcccaga tatataatcc ataaaatttt tgcagtacct gtaactcttg
      541 gagccagctt gtccattttt gggccggggg cttcagcttt gatcaactgc aagccgccaa
      601 tacccaacat gggtaaaaca gcaacggtaa gtaccacaat tcccatacca ccaagccaat
      661 gggtttgtga acgccaaaaa agaattgaac gaggaagtga ctcgatgtcg gttaatatag
      721 ttgcgccggt tgttgtgaat ccggaaataa tttcaaaaaa agcggaagta aaatcaggta
      781 tgcttccgga tatatataga ggtaaggcac ccattgctga agcgctaatc cagcttagag
      841 taacaaacaa aaatccttcg cgagtttgca gctggctctc ttttgttttt cgtgaaaaaa
      901 acaagattgt tgaacataaa ataattattg aagcaataac ataacaaaac gcgcgtaaac
      961 tattgaattc gtgataataa atagcaatta gagccgggac aatcatatta atagctatga
     1021 ttattaataa cgcacataag ggtttaaata tattttcagg cttcatttgc ggtaaaaatc
     1081 ttttctattt gggttatgct ctcttttgtg gtaattatca gaagagagtc attttcggta
     1141 aaaatatagt tgccagtagg tactattttt tcattatttt tgtttatcga aataatcaaa
     1201 gaagaagatg gcatattaat atttttaagc atgatgccaa tagccggact ttttttatca
     1261 agagaaaatt ccagaatttc agctcgaccg ttaaagatac tttttataga gctgacatta
     1321 cctcttctta tatatttaag aattgaatcg acagaagagc ttttcgggct aataactgag
     1381 tcaataccaa gttgcattga aaggttaaga tagttattgt ttacaataag tgcaatggct
     1441 ctttttatac cgcttttttt tgcgtataat gcggacagaa ggttcagttc ttcgttgttt
     1501 gtaagacaaa ttatcagatc atatttgtga accagttcat ctttatatat attctcatct
     1561 gttatatccg cattaataat taatgcttca ggatatattt ctgagagctt tttgcaattt
     1621 ttgtaatcct tgtcaatcaa ggttacattg taattattat taagcaatcg gcttaacacc
     1681 ctagttccaa gaggagttcc acctacgatt aatactttgc gtagtttaat cttcttataa
     1741 tcactaaact tgaacaaacc ctttatattg tgatcgggag ccatgaaata gatattatca
     1801 ttttcattta tgcaggtatt acctgatgga ataattaact gatcttctct taagattgct
     1861 gccacaaaaa aatcaggatc aagggacgtt ctaattgata aaaggctttt accaactaaa
     1921 acagaattat tctgtatcgc catatttatt aggcggacat tacttttttt aaaagatata
     1981 atatgactat gagccccgaa ttcggttatt tctactaatt cgttagccgc ctcggcttca
     2041 gggttaacag cataatctat gtttaaaaat tcttcggaaa taattgaacc gcttaagtat
     2101 tcaggattac gtatccgcgc gattattttt tttacattaa agagggtttt tacaattcct
     2161 gaagcaacca tatttatttc atcagaatcg gttacactaa taaaaaaatc ggctttgata
     2221 attccagctt tcaataaatc cgcgggacta cttgcgctgc cttcaattac catgcagtca
     2281 agtgtagagg aggcatagtt agctcttgat ggatctgatt caatcagaac aacattttta
     2341 ccttctgtta ttagatgctt tgctatttgt gtgccaacgg caccggcacc ggctattact
     2401 atttgcatgc agataactta aaacgattgt tattagtgtc aagatatttt acttgacagt
     2461 ggcaattttt acttaacaga tgttagcgtg aaaagaataa tcatacatat cgatatggat
     2521 gctttttttg ctgctgttga gcaaaacgat aatcctaaac taaaaggcaa gcctgtagtc
     2581 atcggtgccg atcccgaggg cggcaaggga cgtggagtag tgtctacggc ttcttatgaa
     2641 gcacgcaaat atggaataag gtctgctatg ccggtaagcg aggcatatcg aagatgtccc
     2701 gatgcaattt ttattcgcgg taatatgtac aggtataaag aagtctcgga tacaatcatg
     2761 tcggttttta gatcgttttc accagttgta gagcccctta gtcttgatga agcctttcta
     2821 gattgcagtg gttcttcaaa aatttttgga tcaccagttg agattgctca aaaaattaaa
     2881 aactccatat atcagtctac aggccttacc tcttcggccg gagtatcggc ggtcaaatcg
     2941 gtagcaaaaa ttgcttctga cctaagaaaa cccgatggtt tgacaatatg tcaggaaggt
     3001 aacgaaatag agtttttaaa agagttgagt atatctcgtc tttggggtgt tggtaaaaaa
     3061 tcatacaaga tactaactga tatgggtttt tacaaaatag gcgatatcag ttccagaaca
     3121 agaaaagaca tggagcttat tcttggaaga agtggggcag aactatggga tctttcaagg
     3181 ggaatcgatt ttagaccagt tgcattagaa aatgagaggc gaaaatcaat aagtgaagag
     3241 ataactttca aagaagataa aaaatccttt gacgaattaa cgcatacatt aaaatatata
     3301 tgtgacagcc tctctttctc tttatgtaaa gaggcttgta aagcacgcac aataacactt
     3361 aaacttcgtt atgacagttt tgaaacaata actcgtggta aaaccgttgc cgagtgtacc
     3421 agatcttata atgtgatttt caatactgtt ttagaattaa tgggcagttg tcttgtgata
     3481 ggtcgtcgtg tcagattgat cggtgtctcg ttaagtaacc ttgagtatgg aaaatatgag
     3541 gcacagcctg atctgttttc tttaaaagag agtttaattg aatcaccact ggataatgtt
     3601 attaacgatc ttaaaattaa gtatggttct aaaataaaaa gagcgtcact tttacgccat
     3661 gggagagata atgagtaaag agaataaata catagtattc gatatcgaat ctgtaccgga
     3721 tggaaagctt ataaaagatg tgaagtatcc tggagaagac ataagtgaag acgaagcaat
     3781 ttccagactt caggatgaga tcatggataa aacagccggc aaatccaatt ttataccggt
     3841 aactatgcaa tatcctatat gcgtggtagt tgctctaatt gcagaagatt ttacagtaaa
     3901 cgaaattaaa tcgctggatt atggagagta cagagcttct gaaatcgcaa gaagcttctg
     3961 gttcagagtg gaagttcttg ataagaaggc gtctcttgta acgtttaatg gacgcggttt
     4021 tgatatacct atcatggaac ttatggctta tcgctacggc catgatatga aacgtcatct
     4081 cactgacaaa tttggaacac gcttccgctt tggtacacgc catattgatc ttcaggattt
     4141 tatcggtaac tacaacgcta ttggtgtatt cggtggactc aatatgcttg ctaaaaccat
     4201 aggtttgcct ggcaaaacag atactgccgg cgatcaggtt tacgacctat ataaagaagg
     4261 aaaaatagag cagataaatg actattgtat tcatgatgta ctggacacat atctggtttt
     4321 tttacgtaca agggtaatgc tgggagagat cactcttgat gacgaaaaga aaattcgtga
     4381 aaactgtttc tcttatctaa aagagagttg tgaatataaa aacgccgcca ttagttatct
     4441 ttcgcatata aaggattttg aaccatggcc ttgatacgtg cttagactac gcaagttcct
     4501 gttgataaat taattgttag tggataccaa tagtttcaaa taaattattc taaagttgag
     4561 gtcttaatgt caaaatcacg tgtatggatt attacaggtg cagcaagagg tatgg
//
LOCUS       DAOTGZ010000216         4561 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_3064
            NODE_22523_length_4561_cov_4.918997, whole genome shotgun sequence.
ACCESSION   DAOTGZ010000216 DAOTGZ010000000
VERSION     DAOTGZ010000216.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868088
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 4561)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4561)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:22:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,785
            CDSs (total)                      :: 2,751
            Genes (coding)                    :: 2,732
            CDSs (with protein)               :: 2,732
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 12 of 19
            Pseudo Genes (internal stop)      :: 5 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4561
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_22523_length_4561_cov_4.918997"
                     /isolate="AMR_MDS_3064"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            240..629
                     /locus_tag="P5123_11620"
     CDS             240..629
                     /locus_tag="P5123_11620"
                     /inference="COORDINATES: protein motif:HMM:NF017020.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HEPN domain-containing protein"
                     /protein_id="HRX16952.1"
                     /translation="MDNKEHIQYWLNSAEHDLDSADALFSAGKYDWCLFLGHLVVGKA
                     LKAKCVSVNDEFPPKSHNLLFLCEKAKIELTDGQKEFLDIVNGFNIDTRYPDYKLTFY
                     KNCSKEFTLKHFTKIKEIYRWIRSLII"
     gene            605..922
                     /locus_tag="P5123_11625"
     CDS             605..922
                     /locus_tag="P5123_11625"
                     /EC_number="2.7.7.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013290234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase domain-containing
                     protein"
                     /protein_id="HRX16953.1"
                     /translation="MDKIPNNIAEIINKYLLSLKENNLIISQAYLFGSYAKGTYEEYS
                     DIDIALVSAKFCGDRMADRGMIRKSTIRNSPLIEVIPFAESDFTMENPFAKEIMNHGL
                     RIA"
     gene            complement(1049..2392)
                     /locus_tag="P5123_11630"
     CDS             complement(1049..2392)
                     /locus_tag="P5123_11630"
                     /EC_number="3.2.1.21"
                     /inference="COORDINATES: protein motif:HMM:NF012457.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008422 - beta-glucosidase activity
                     [Evidence IEA];
                     GO_process: GO:0030245 - cellulose catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GH1 family beta-glucosidase"
                     /protein_id="HRX16954.1"
                     /translation="MKYNFPDDFLFGAATASYQVEGAVNEDGRSPSVWDVFSHTPGKV
                     KNNDNADISCDQYHKYKEDILLMKDIGLQTYRFSTSWSRVLPDGRGKPNQKGLDYYNN
                     LVDGLLEAGIEPWVTLFHWDFPQVLQDEYKGWLSRKSIDDFTEYASLMAKTLGDRVTN
                     WFTMNEFDCFTDKSSLRQEFAPDFKATGKEAAQIRHNAMVAHGKAVLAIRASSPGTVR
                     VGMAENSLCTIPVFETPEHIAAAKKAFRDINARFTTPIMEGAYDPGYLEYIGSNAPDI
                     EPGDMETMSTKTDFFGLNMYTPVYIKAGGELGWQEIHMSADHPKAKPAWLRINPSIGY
                     WTTRFIKELWNVNEIYITENGASFDDIPDQNGKVNDTARIMYLREHLKGIHRAIDDGI
                     PVKGYFLWSLLDNFEWKDGFDERFGIIHVDYKTLKRTPKLSAEYYSEVIKNRSIV"
     gene            complement(2650..3252)
                     /locus_tag="P5123_11635"
     CDS             complement(2650..3252)
                     /locus_tag="P5123_11635"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRX16955.1"
                     /translation="MQPSSKLIESETVSEYRNTETSNRDRIEHLFFENFDLFLRKTDL
                     INANPAMKLYNSHLFVFGGYPVQAVSVYLGTMLRAWNDGQFSESCDCGKTAYVYSCGG
                     SLTSGTVGWRYSYCPDCKKHYNHKKPGSVGLYFKIADNIMRGCSNIKKVKHLVEIPLE
                     YRYGKVIEHNLKPSHSEVIINEPVQTLTMENIVKILKEAD"
     gene            3615..4241
                     /locus_tag="P5123_11640"
     CDS             3615..4241
                     /locus_tag="P5123_11640"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRX16956.1"
                     /translation="MNYIHKTGAFSLFGGSVLLVIYSILVSIMMKGFDGTGDFCNVIR
                     HDYWMVATATVFAGLVLLMLGFAVVYSRIASQSGALGFAGIVVFEFAYLLQAAKVTWE
                     IFIYPQLAYNDNASFLISDRIIWNSMSIMVYLILSSVTLLLGIIIFCVSLYRSGYYTK
                     LSSFMLLIGAVMYGAGELIHMTVAIVGIVIFAAGCFLCGMTLLRKQLT"
BASE COUNT         1268 a          815 c          974 g         1504 t
ORIGIN      
        1 gttcgccagc ttggattagg aaaaaattgc agtaattgtt agattttgct ttgagcagta
       61 tctcggtttc gcactccccg gcaccataat ttaaaaaaaa ttatgatgct gggcaggctg
      121 gacagtgggc gtattggttc tttggttttt tttcaatagt aggtatcagt cgcgactgat
      181 acctactatc gaaaaataca ttcttgacaa atttgtgaac tgatcttaat tttttagtta
      241 tggataataa ggaacatata cagtattggc ttaacagtgc tgaacatgat ctggattctg
      301 cagacgcatt gttttctgcg ggcaaatatg actggtgtct atttctcggg catcttgttg
      361 tcgggaaagc tctaaaggca aaatgtgtca gtgttaatga tgaattcccg ccgaaatccc
      421 acaatttgtt atttctctgt gagaaagcaa aaattgaatt aactgatgga caaaaagagt
      481 ttcttgatat tgtgaacgga ttcaatattg acacaagata tcctgattac aaattgacat
      541 tttacaaaaa ttgttcaaaa gaatttacac tcaaacactt tacaaaaatc aaggagatat
      601 accggtggat aagatcccta ataatatagc tgaaataatt aataagtatt tgttgtcgtt
      661 aaaagaaaat aatcttatta tttcacaggc atatcttttc ggcagttatg cgaaaggtac
      721 ttatgaggag tatagcgata tcgacattgc tttggtctcc gcgaaatttt gtggagatcg
      781 tatggcggat agaggcatga tccgaaaaag tacgataaga aattcaccgc tgatagaagt
      841 tatccctttc gcggaaagtg actttactat ggaaaatcct tttgcaaaag agataatgaa
      901 tcatgggctg cgaatagcct gaatatttac accgttctca agtgaaaata ttcaggcgtt
      961 ttcgggtgat cagcccgaaa attttccaaa taatagagtg ccagcgacgt gaagtggtga
     1021 ctttacgtga tctagaagtg gcgatttgtc agacaatcga cctgttcttt ataacttctg
     1081 aatagtattc agccgaaagt tttggtgtgc gttttaaagt cttgtagtcg acatgaataa
     1141 ttccgaaacg ttcgtcaaag ccgtctttcc actcaaaatt atccagcagg ctccataaga
     1201 aatatccctt aacggggatc ccatcgtcaa tggcacggtg aatacctttc aggtgttcgc
     1261 gcaaatacat gatgcgtgct gtatcgttaa ctttcccgtt ctggtcaggg atgtcatcaa
     1321 aagaggcgcc gttttctgtt atataaattt cgttaacgtt ccacagttcc ttgataaaac
     1381 gggtagtcca gtaaccgata gaggggttga ttcttagcca cgcgggcttg gccttcggat
     1441 gatccgcgga catatggatc tcttgccagc ccagctctcc tccggcttta atatacacag
     1501 gagtatacat attaagtccg aaaaaatctg ttttagtgct cattgtctcc atgtcaccag
     1561 gttcgatatc gggtgcattt gatccaatgt attcgagata tccagggtca taggcgccct
     1621 ccattatcgg agtggtaaaa cgtgcgttta tatcacgaaa cgccttttta gcggcggcaa
     1681 tatgctccgg ggtctcaaac acaggtattg tgcatagaga gttttcggcc attcctacac
     1741 gcacagtgcc cggtgatgag gcacgaatcg ccagcacggc ttttccgtgc gcgaccatgg
     1801 cattatgccg aatttgtgcg gcctcttttc cggttgcctt aaaatcggga gcgaattcct
     1861 gtcttagtga tgatttatct gtaaaacagt caaattcgtt catggtaaac cagtttgtta
     1921 cccggtcgcc caacgttttg gccattaaag aggcatactc ggtaaagtca tctattgatt
     1981 tgcgtgaaag ccagcctttg tattcgtctt gaagaacttg cggaaaatcc cagtgaaata
     2041 acgttaccca gggctcaata ccagcttcaa gaagtccgtc tacaaggttg ttgtaatagt
     2101 ctaacccctt ttggttaggc ttgcctcgtc catcgggtaa tacgcgggac catgatgtcg
     2161 aaaatctgta ggtctgaagt ccgatatctt tcattaatag gatatcttcc ttgtatttgt
     2221 ggtactgatc gcagctaatg tcggcattgt cgttgttttt taccttgccc ggtgtatgtg
     2281 aaaaaacatc ccacacactt ggtgagcggc cgtcttcatt aaccgcgcct tctacctgat
     2341 agctggcagt tgctgctccg aaaagaaaat catcgggaaa gttgtatttc ataatagtct
     2401 ctcttttttg tttttatctg aataaaacac gcattctttt ggtcaaggta aaatatgctt
     2461 agctgattaa aacttttcgg tagcaaatca acattgcagt tgttttttga agctttaaca
     2521 aagacaaata taacaggtgt gatattgtgt caagatcata gtggtttttc attcctaaag
     2581 ggggtgggcg gagagtcctt aattgaaaaa gagttacaga taaggactct cctgaattga
     2641 aaaaaaatat taatcggcct cttttagaat tttgacgata ttttccatgg ttaaagtttg
     2701 taccggttcg tttataataa cttcactatg gctgggtttt aagttgtgct caataacctt
     2761 tccatatcta tattccaacg ggatctctac tagatgcttt acttttttta tattagagca
     2821 gcctctcata atattatcag caattttaaa atagaggcca acgcttcccg gttttttgtg
     2881 attatagtgt tttttgcagt ccgggcaata tgagtagcgc caacccacgg ttcccgatgt
     2941 caaggatcct ccacatgaat agacatacgc agttttgcca cagtcacagc tttcagaaaa
     3001 ctggccgtcg ttccatgctc ttaacatggt acccaaatag accgatacgg cttgtactgg
     3061 gtagcctcca aaaacaaaga gatgagagtt gtaaagtttc attgctggat tggcgttgat
     3121 aagatcggtt tttcttaaaa acaggtcaaa gttttcaaaa aaaagatgtt caattcggtc
     3181 tctgttagaa gtttccgtgt tacggtactc gctgactgtt tctgattcga ttaattttga
     3241 tgatggttgc atatgttacc tccggtttgt tttgtgcggc tttaagccgt aaataaatac
     3301 cgaagattga acggaaggga aataggaatt aatttcgctt tagcccaatc ttttaaagtg
     3361 gatggcaagc tgaataatca aattctccac tatttatatt ataacacatt gatatagttt
     3421 tgtcaaatga ttttagcaaa gttaagagat ttgatttgcc tgcgaatttc ttgaaggggt
     3481 attattgttt cagcacatag gtgctgtgta aaagtcatta aaagtggtta atttggttat
     3541 gtaaatctgt tgacaaaaga taataagatt atttaaagaa atatagatta attgaaaaaa
     3601 atttggagaa aaaaatgaat tatattcata aaactggtgc tttttcactc ttcgggggat
     3661 ctgttttact tgttatatat tcaattcttg tttcaataat gatgaaagga tttgatggaa
     3721 cgggtgattt ctgtaatgtt atacgacatg attattggat ggtcgctaca gcaaccgttt
     3781 ttgccgggct tgttctatta atgttggggt ttgcagttgt ctactcccgt attgcttcac
     3841 aaagtggagc tttgggtttc gctggaattg ttgttttcga atttgcctat cttctgcagg
     3901 ccgctaaggt aacatgggaa atatttatct acccgcagct tgcctataat gataatgcat
     3961 cttttctaat aagtgataga ataatctgga atagtatgtc aattatggtg tatctgattc
     4021 tgtcgtctgt gacattgctt ttaggaatca ttattttctg tgtttcactg taccgatcgg
     4081 gttattacac aaaactgtcc tcgtttatgc ttctgattgg cgctgttatg tatggcgctg
     4141 gcgaattgat tcatatgaca gtagccatag taggaattgt aatatttgcg gctggatgtt
     4201 ttctttgcgg tatgactctg ttaaggaagc aattaactta aaataatcta ttattccgac
     4261 cgtgtactgg tatagtgaat agagttttgc ttttgtttag tttaacgttt tttgcaaaca
     4321 aaatcgtggg cgtcctttgt cagggcgctt tttgttcgtt attttggtgg tgtgtcgtgt
     4381 tttttttgtt ttgcttctgt actatttagt acaaaagctc agattatttc aataaataca
     4441 gtggtttcct ggtttcttgt ccagaaggaa tatcttgacc ctcgtaaaag ttcatacccc
     4501 agctgtttat gaagtaggga aatccgaaga agtgatgaac aaaagctgca tgaaaaacag
     4561 g
//
LOCUS       DAOTGZ010000217         4541 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_3064
            NODE_22644_length_4541_cov_4.246545, whole genome shotgun sequence.
ACCESSION   DAOTGZ010000217 DAOTGZ010000000
VERSION     DAOTGZ010000217.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868088
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 4541)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4541)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:22:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,785
            CDSs (total)                      :: 2,751
            Genes (coding)                    :: 2,732
            CDSs (with protein)               :: 2,732
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 12 of 19
            Pseudo Genes (internal stop)      :: 5 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4541
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_22644_length_4541_cov_4.246545"
                     /isolate="AMR_MDS_3064"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..182)
                     /locus_tag="P5123_11645"
     CDS             complement(<1..182)
                     /locus_tag="P5123_11645"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRX16957.1"
                     /translation="MIKGAARGNENPGKESLSINELIKENPDIVAIIEELGIPRKGCE
                     AQSLSFLCEEAGVDPD"
     gene            complement(179..892)
                     /locus_tag="P5123_11650"
     CDS             complement(179..892)
                     /locus_tag="P5123_11650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020612984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-binding protein"
                     /protein_id="HRX16958.1"
                     /translation="MKLIIVGGPASSGKTSVIIHALNNPIIDKSKVLVAKLDCLVAND
                     SEVYKKNKIASITGLSTYICPDHYLSTNIERISKYGIKKRVDYLIIESAGLCNRCSPY
                     LTDTLGVTILDVLSGIQSPNKIGPMLKSADIVVLTHCDMISQAEREVFRKRISVLNCS
                     AKILEVNGLTGKNSYRLSGEFALAKEFDASVPQHLKYSMPGAVCSFCLGEKRVGETFA
                     SGNVKLMELPGIKDGVWKK"
     gene            complement(889..2163)
                     /locus_tag="P5123_11655"
     CDS             complement(889..2163)
                     /locus_tag="P5123_11655"
                     /inference="COORDINATES: protein motif:HMM:NF024739.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HRX16959.1"
                     /translation="MKNNKLKRNQNTLSLSPDMKIADVIRLWPQTLNTFIRMGFTPLK
                     NPLLRKTVAGLVTIEKAAFTRNIDLNILMLNLQNSITNPTERNEGFIDPDKLRYDENT
                     IPDLNGEINLVGLIPCPISNIVTEMFDAFIQKNYSSKKTRLAWWIAAEGGGTVDIKNF
                     IVSIAKSGQYDSFPDYFMAVGTELFLHDDYCRKMYHEDVFIDGNSTSYKRPEFEKLED
                     PNKKLNLQFLVYFTFYCTPETMDGLPLPATWFDLTNPEYKGKIVLPSLNLPVVPDFLA
                     ALYYHLGDSLFVQFCRNVSFALHPSQSSSRKVKKNSPSIFITPIHFSKIIKSKGHVHI
                     IPEDGPVAVPAYYVKTSSKPKAHSIENFLTSKELMEVYYKNGSFIPNRKDILIEYPLD
                     NIITRPWTSLFDIIPDNLLEQLLQNFKLGESL"
     gene            2271..3512
                     /locus_tag="P5123_11660"
     CDS             2271..3512
                     /locus_tag="P5123_11660"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRX16960.1"
                     /translation="MKSQSDKVYITSIGSCTASGNSEDIFFNAKNKKSSLTVYNQITD
                     DCPVKTVGKVKLKTDDIFAQKVRPFVSRNAELGAYAVSQCLNKIELSRTIPDGLIFGS
                     ASQGIDKIMEVIDNLKDSPFNTLNHSNISSITNAGVCRLLAERFSISGRVFAVEGASC
                     SGMIAFIESFNAIKNKIASNMLAVSGEANLFKPAFLFYNKKVRVNKETYTFFGKVGES
                     IFRRPSEAVVPYCIPEKSDRGVIAEGGAALFLANENHIKEFDLPVFAEIENVFYYYNC
                     TLSSNNDFLGLSNILDKLKNEIFDSIYLPISGCWATDYNLYTACSHYFPNTHCFSVEP
                     VIGHTGAVSGLINIILGALSLKNSILLPTLNFDGKNLDQGFKLKPSEVIIEKDIQRIL
                     VISSGWGGYNGACVLKKYNGK"
     gene            3502..4152
                     /locus_tag="P5123_11665"
     CDS             3502..4152
                     /locus_tag="P5123_11665"
                     /EC_number="2.1.1.100"
                     /EC_number="2.1.1.334"
                     /inference="COORDINATES: protein motif:HMM:NF018649.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isoprenylcysteine carboxylmethyltransferase
                     family protein"
                     /protein_id="HRX16961.1"
                     /translation="MANEKFRLDLLALVMMTSLLLWGFLFIVPVINIKKYIIAAFLLN
                     VSLLIEFVFNVGFQTEKTNDSGFLSLTLFSLVIITTAYYRYLNLPFLIALSIWNNIFV
                     LGIILIIIASAIRIVSKIYLKESFSYNIITTQNQKLITTGPYSIIRHPGYFGTILAIL
                     GATFMYANPITLLFIILSLPVTVKKIRKEERLLVKLFDNEYINYQAKTKMIVPFIL"
     gene            4152..>4541
                     /locus_tag="P5123_11670"
     CDS             4152..>4541
                     /locus_tag="P5123_11670"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF015022.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016758 - hexosyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0030259 - lipid glycosylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HRX16962.1"
                     /translation="MKIKFKKILLCAMGTFGDIAPLLAYGLQLKKAGHTIAFCSANNY
                     KNIADVYNIIFYSIDIDFDLIKNENAKAMGRSILSLLKFKQNLITFSQYEFSTLTEII
                     KKHDEVIASGAVLSAMSICEKHNIPYKH"
BASE COUNT         1481 a          841 c          762 g         1457 t
ORIGIN      
        1 tcatccggat ctactccggc ttcctcacat aaaaaagaaa gtgactgtgc ctcgcaacct
       61 ttacggggaa tacccagctc ttcaattatt gcaacaatat caggattttc ttttataagc
      121 tcatttattg ataaagattc ttttcccgga ttctcattac ctctcgccgc tcccttaatc
      181 attttttcca caccccatct ttaattcccg gaagctccat caacttaaca ttaccgctcg
      241 cgaaggtctc ccccaccctt ttttctccaa gacaaaaaga gcatactgct ccaggcatag
      301 agtacttcaa atgttgagga acagaagcat caaactcttt cgcaagagca aactcgccag
      361 atagcctgta actgtttttt cctgtaagac cattcacctc aagaattttt gcgctacaat
      421 ttaaaacaga tattcttttt ctgaacacct cacgctcggc ttgacttatc atatcgcaat
      481 gcgtcagcac aacaatatca gcagacttta acattggacc gatcttattt ggagattgaa
      541 taccgcttag aacatccaga attgtgactc caagcgtatc agtcaaataa ggcgagcatc
      601 tgttacacag acctgcactt tcaattataa gataatcgac tctttttttt ataccatatt
      661 ttgaaatacg ttcaatattt gtcgaaagat aatggtcggg gcaaatatat gtcgaaagac
      721 ctgttatgga agcaatttta ttttttttgt aaacttccga atcattagcg acaagacagt
      781 caagtttagc aaccaagact ttactcttat ctattatagg attatttaga gcgtgaataa
      841 tcacagatgt cttaccagat gaggcaggtc ctccaacgat tatcagtttc atagcgattc
      901 acctaattta aaattctgca gcaactgctc cagtaaatta tcaggtataa tatcaaaaag
      961 agacgtccag ggtcttgtta ttatattatc gaggggatat tcaattaata tatcctttct
     1021 gtttggaata aatgatccgt ttttataata gacttccatt agctcttttg atgttaaaaa
     1081 gttttcgatg ctatgagcct tcggctttga agatgttttt acataatagg caggtactgc
     1141 aaccggacca tcttcaggga taatatgtac atggcctttc gatttaatga tctttgaaaa
     1201 atgaattggt gtaataaaga ttgaaggcga attttttttc accttccttg aagaagattg
     1261 tgaaggatgt agagcaaaac tcacatttct gcagaactgt acaaaaagag agtcacccag
     1321 atgataatat agggcggcaa gaaaatcggg aaccaccgga agatttaaag agggcaaaac
     1381 aattttgccc ttatattcag gattggttaa atcgaaccag gttgccggta atggaagacc
     1441 atccatggtt tctggagtac agtaaaatgt aaaatagaca agaaactgta aattaagttt
     1501 tttgttagga tcctcaagtt tttcaaattc gggtcgtttg taactggtac tgttaccatc
     1561 aataaataca tcctcatgat acatttttcg acaatagtca tcatgtaaaa aaagctctgt
     1621 gcctaccgcc ataaaatagt ccggaaaaga atcgtactgt ccggattttg ctattgaaac
     1681 aataaaattt ttgatatcaa ctgttccacc accttcagcg gcaatccacc aggctaatct
     1741 ggtcttcttt gatgaatagt tcttttgaat aaatgcatca aacatttcag ttacaatatt
     1801 actgattggg caagggataa gaccaacaag atttatttca ccgtttaaat caggtatcgt
     1861 gttttcgtcg tatctaagtt tatcgggatc tataaagccc tcatttcgct cggtggggtt
     1921 ggttattgaa ttttgtagat taagcataag tatattaagg tcgatattgc gcgtgaatgc
     1981 cgctttttca attgttacca agccagcaac agtttttcgc agaagtggat tctttaacgg
     2041 tgtaaacccc atcctgataa atgtatttaa tgtttgtggc cacaacctta taacatcggc
     2101 aattttcata tccggagaaa gggatagtgt attttgattt ctttttaatt tattattctt
     2161 cataactaaa catctatcga ttgcaaattt gataaagtca atcaaaatac aaattatttt
     2221 taataagcaa atattcttga ctatcgatta aatgattatt taatgctttt atgaaaagcc
     2281 aatccgacaa agtatatatt acatctattg gttcatgtac tgcttcagga aattccgagg
     2341 atattttttt taatgccaaa aacaaaaaat catcacttac agtttataat caaatcacgg
     2401 acgattgccc agtaaagacc gttggcaagg ttaaacttaa aacagatgac attttcgcac
     2461 aaaaagtccg cccttttgta tcgcgcaatg ccgagcttgg agcatacgca gtatcccaat
     2521 gtctgaataa aatcgaatta tcgcgtacta tacccgacgg tcttatcttc ggatctgcat
     2581 cccagggcat agacaaaatt atggaagtaa ttgataattt gaaagacagc ccttttaata
     2641 cccttaacca tagcaatatc agctcaatta caaatgccgg tgtttgccga ctattggcag
     2701 agaggttctc gataagtgga agagtgtttg cggttgaagg tgccagctgc tcgggcatga
     2761 ttgcatttat tgagtcattt aatgccatta aaaacaagat cgcaagcaac atgttagccg
     2821 tatcaggaga agctaacctt tttaaaccgg ctttcctgtt ttataataaa aaagtcagag
     2881 taaacaaaga aacctatacc ttcttcggaa aagttggtga atccatattc aggcgaccat
     2941 ctgaagcggt tgttccatac tgcattcctg aaaaaagtga caggggcgta atcgcagaag
     3001 gaggtgccgc gttatttctt gcaaacgaaa accatatcaa agagtttgat ttgccggttt
     3061 ttgccgaaat cgaaaacgtc ttttattatt ataactgcac attgtcatcc aacaatgact
     3121 ttcttggact ttctaatatt cttgataaat tgaaaaatga aatatttgat tcaatatatc
     3181 ttccaatttc cggttgttgg gcgactgatt acaatttata tactgcttgc agccattatt
     3241 ttcccaacac acactgtttt tcggtcgaac cggtaattgg tcatacggga gcagtatctg
     3301 gactaataaa tatcattctt ggagccttaa gcttaaaaaa cagtattcta ctaccaacac
     3361 ttaactttga tgggaaaaat ctggatcaag gctttaaact caaaccatct gaagttatta
     3421 ttgaaaaaga tattcaaaga attctggtaa taagtagtgg ttggggtggc tacaacggag
     3481 catgcgtgtt aaaaaaatat aatggcaaat gaaaaattta gattagactt attagctctt
     3541 gttatgatga cctcattatt gctttggggg tttcttttta tagtaccagt tataaacata
     3601 aaaaaataca ttattgcagc ttttttatta aacgtatcac tactgattga attcgttttt
     3661 aatgtgggtt ttcaaaccga aaaaactaat gacagtggtt ttttaagttt aaccctcttc
     3721 tcacttgtta taatcacaac tgcctattac agatacttaa atcttccatt tttaatcgca
     3781 ttaagtatat ggaacaatat atttgtttta ggaattatat tgatcattat tgcctctgcc
     3841 ataagaattg tttccaaaat ttatctaaaa gagtcttttt cttataatat aataacaaca
     3901 caaaatcaga aacttatcac aactggtcct tattcaataa ttaggcaccc tggatatttt
     3961 ggaacaatac ttgctattct tggagcaact tttatgtacg ctaatccaat tacccttttg
     4021 tttattatac tttctttgcc agttacagta aaaaaaataa gaaaagaaga acgactactt
     4081 gtaaaactat ttgataatga gtacataaat taccaagcaa aaactaaaat gattgttcca
     4141 tttatactat aatgaaaata aaattcaaaa aaatattatt atgcgccatg ggtaccttcg
     4201 gggatattgc accacttctt gcttacgggc ttcaacttaa aaaggcaggc cacacaatag
     4261 ccttttgctc cgcaaataat tataaaaata ttgctgatgt atacaatatc atcttctatt
     4321 caatcgatat agattttgat ttgataaaaa atgagaacgc taaagcaatg ggacgatcca
     4381 tactcagttt attaaaattt aagcaaaatc taattacatt ttcacaatac gaattttcga
     4441 ctcttaccga aataatcaag aaacatgatg aagttattgc aagcggagct gtgctttctg
     4501 caatgagtat ttgcgaaaag cacaatatac catacaaaca t
//
LOCUS       DAOTGZ010000218         4520 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_3064
            NODE_22775_length_4520_cov_4.495633, whole genome shotgun sequence.
ACCESSION   DAOTGZ010000218 DAOTGZ010000000
VERSION     DAOTGZ010000218.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868088
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 4520)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4520)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:22:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,785
            CDSs (total)                      :: 2,751
            Genes (coding)                    :: 2,732
            CDSs (with protein)               :: 2,732
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 12 of 19
            Pseudo Genes (internal stop)      :: 5 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4520
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_22775_length_4520_cov_4.495633"
                     /isolate="AMR_MDS_3064"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1501
                     /locus_tag="P5123_11675"
     CDS             <1..1501
                     /locus_tag="P5123_11675"
                     /inference="COORDINATES: protein motif:HMM:NF012245.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein"
                     /protein_id="HRX16963.1"
                     /translation="AVLTAKVDAEWLSLAADNIMYGANGYSYIIDEKGTIIGHHNREF
                     VLKKRNFIKESETDDNYSQIATMFKQMVSGESSYQDIHFDGTDSFFGFSPIGDTGWSI
                     AVGTDKSNVFRPIENMRKWIIIVSAIFIIAGIIFAYYVARSITTPILTAVETIKELSE
                     GNLTQRIIAKGKDEIADMAVHFNSFIQKMHTIISSISKSASSLSTESDEISASASTFS
                     ELAQNQAASAEQISATIEEVSAGIENINTESQDQYKSLGEFIIHMKTLYDNMNNMARN
                     LKETLTISADIANTAKSSEDSLKSMTSSMTSILDSSREMTGIVDIINDISEQINLLSL
                     NASIEAARAGESGRGFAVVADEISKLADQTASSIKEIDKFIQQNNEQIDRGQQSVKSA
                     TATISRVIESIEKISEMVNHLGDIMKTQLEVNETVNAEALRMQQRSEQMKGATEEQKN
                     AISEIVHSITSINENTQANATGAIQMAGTVEKLSEMAEELKNDVDFFRT"
     gene            2004..>4520
                     /locus_tag="P5123_11680"
     CDS             2004..>4520
                     /locus_tag="P5123_11680"
                     /inference="COORDINATES: protein motif:HMM:NF037694.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004866 - endopeptidase inhibitor activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MG2 domain-containing protein"
                     /protein_id="HRX16964.1"
                     /translation="MKLPSLSAIAKKISTHRFIIAGGAVAMTSIAAIAMTAVLTLTAD
                     RAPGAPDVFWASHIKGHTGGEISKTSTINVIFNNNIVDKADIGKEVDGVFSFDPSLKG
                     KAEWVSQNELVFVPEKHMPAGEKFLCTLNLDDYIKKTDGVDDFFFGFSIIEPNFDIKL
                     GGFRFPKLENLNIQDYEGSIKTADFEDSKAIEKILEASQKGKNLEIEWKHSSETKTHN
                     FTIKNISRGKSPSELLLKWDGSSINSDRSGEHRIEIHARDVFKVSSIEVISSSEKYVE
                     LTFTDPLKQNQNLRGLIYFEDIDSRTEIDMNIVRVFPTGDKIGDADIRVERGVLNSEG
                     KRLNEAFHRRITFQKIDPGVRFIGDGTILPDTDNLTVPFEAVNVHSLQVTVFKVYDKN
                     MGQFFQVNDFKQNNEIKRVGRYLWRKTIKLNATPAELSKWSRYSLDVSSLYKEHPGSL
                     FRIELSINRSNSALGCSDNPDPAIVEPDFTNQEATIISDSSYWDYWEEYDHSEYWDNR
                     KNPCHDAYYKQSYGNPVFSAKNFLSSNIGITAKYGSGKTLTVVTTDIVDASPTSASIK
                     IYNFQNIEIGSGSTDGNGFADITVSGEGDPFYIVAEKRGQKGYLKLSGASQLIISHFD
                     VGGEKVKNGLKGYIYGERGVWSPGDNIYLTFVLEDKDNLLPKDHPITMKLYDPRGRLK
                     QIQSAYRQANNFYAFELKTDSEDETGSWTARAELGGLSFTKTLKIETVKPNRLKVELD
                     IGGKELHRSKMPVSAKINSQWLHGATAQNLTTDVSARFSGLTTEFSRYTDYVFDDLTR
                     TASSEKTEIFSGSLDSTGKASFNISFSDNSKPAGMLNAYFT"
BASE COUNT         1489 a          901 c          924 g         1206 t
ORIGIN      
        1 agccgtattg acggcaaagg ttgatgccga atggttatca cttgctgcgg acaacattat
       61 gtatggcgca aacggttatt catatataat tgacgaaaaa ggaacaataa tcggtcatca
      121 taaccgtgag tttgtgttaa aaaagcgtaa tttcataaaa gaatcagaaa ccgacgataa
      181 ctattcccaa atagcaacga tgtttaaaca gatggtctcg ggtgaaagca gttatcagga
      241 tattcatttt gacggcacag acagtttttt cggtttttca cctattggtg atacaggttg
      301 gtcaatcgct gtcggtacag acaaaagcaa tgtgtttcgt ccaattgaga acatgcgcaa
      361 atggatcatt attgtttcgg caatatttat tattgcagga attatttttg cctactatgt
      421 cgcgcgctca ataacaaccc ctattttaac tgctgtcgaa accattaaag agctatcaga
      481 aggcaacctt actcagcgaa taatcgcaaa aggcaaggat gaaattgccg atatggctgt
      541 tcatttcaat tcatttatac agaagatgca cacaataatt agcagtatct ccaaaagcgc
      601 ctcatccctt tcaacagaat ctgacgagat ctccgcctcg gcttcgacct tttctgaact
      661 tgcccaaaac caggcggcat ccgcggaaca gatatcggct acaatagaag aagtttcagc
      721 cggaattgag aatattaata ctgaatctca ggatcaatac aaaagccttg gtgaattcat
      781 tattcacatg aaaactcttt atgataacat gaataatatg gctcgaaacc ttaaagagac
      841 acttacaata tctgccgata ttgccaatac agcaaaatcc agtgaagatt ctctaaaatc
      901 catgacgagc agcatgacaa gcatacttga cagctcccgc gagatgacag gtattgttga
      961 tattattaac gacatatctg aacaaataaa ccttttatca cttaatgcat ccatagaggc
     1021 ggctcgcgcg ggcgaatcgg gaagaggttt cgcagtagtt gcggacgaga tttctaagct
     1081 tgctgatcag acagcatcaa gcatcaagga gatagacaag tttattcagc agaataacga
     1141 acagattgac cgcggccaac aaagcgtaaa atcggcaaca gctaccataa gccgtgtaat
     1201 tgagagtatc gaaaaaatta gcgagatggt aaaccatctg ggagacatca tgaaaaccca
     1261 gcttgaagta aatgagacag ttaatgccga agccctgcga atgcagcaaa gatctgaaca
     1321 gatgaaaggc gcgactgaag aacagaaaaa cgccatcagc gaaattgttc actcgataac
     1381 atcaattaac gagaacaccc aggcaaacgc aacgggagcc atacagatgg caggcactgt
     1441 tgaaaagcta tccgagatgg cagaagaact taaaaatgat gtggattttt tccgaacata
     1501 aaaatagaat cgtaacgtct cgttaaagcg aatagacaca aaccttttct caattttctt
     1561 cattatttat gacagcgtat gctgatattt tgttaatacg gttctatcct tgaaattgtc
     1621 accaaaacca gccccaagat agctgattat tttcattatt acttgcaggc gttaacaaaa
     1681 aacggcttga ccttcactct ttttaatata taaaatccga tagtttcttt ttttaggctg
     1741 cttaaacagt taaataacca ggcggtaatc aacagagctt ctttcttatt attattatgg
     1801 taatctttaa tttggcttta aacggcatag ccgttccaga aatggtaatt attcgaagtt
     1861 tccttatatc agaaagcttt atccagtcgc tacaatttta gaacggcaca cactaagact
     1921 atgcgaataa atttactatt gtgaatgcct gtgtaacgct ttttttaaaa aaaccagaaa
     1981 aacaaaaacc ttgaggtatt caaatgaaac tcccttcttt atcagctatt gcaaaaaaaa
     2041 tcagcaccca ccgttttatt atcgcaggtg gagcggttgc catgacttct atcgcggcca
     2101 ttgccatgac agctgtgtta acattaacag cggacagagc cccaggagcg cctgatgtct
     2161 tttgggcttc tcatataaag ggacatactg gcggagaaat atctaagacc agcacaataa
     2221 atgttatatt taacaacaat attgttgata aggccgatat tgggaaagaa gttgacggag
     2281 tattttcgtt tgatccgtct ttaaagggaa aagctgaatg ggtctctcaa aatgaacttg
     2341 tttttgttcc cgagaaacat atgcctgctg gtgagaaatt cctgtgcacg ctaaaccttg
     2401 acgattatat aaaaaagaca gacggggttg atgatttctt ttttgggttt tcaattattg
     2461 aacctaattt tgatataaaa ctgggaggct tcaggtttcc aaagcttgag aatcttaata
     2521 ttcaggatta tgagggctct ataaaaacag ccgattttga agacagcaag gcaattgaaa
     2581 agatactcga agcctctcag aaaggcaaaa atctggaaat tgaatggaaa cactcttcag
     2641 aaaccaagac ccataatttt accataaaaa acatatcccg cggcaaaagt ccctcggagc
     2701 ttctgcttaa atgggacgga tcttcaataa attctgatag aagcggcgaa cacaggattg
     2761 agattcatgc tcgtgacgta ttcaaggtca gtagtatcga agtgatttcg agctctgaaa
     2821 agtatgtcga gctgacattt acagatccac taaaacagaa tcagaacctt cgtggtctta
     2881 tttactttga agacattgat tcgcgaaccg aaatagatat gaatattgta cgcgttttcc
     2941 caactgggga caaaattgga gatgccgata taagagtcga aaggggtgtt ttaaacagtg
     3001 aaggtaaaag actaaacgag gcttttcata gaagaattac atttcagaag atagatcccg
     3061 gcgtacgttt tattggcgac ggcacaatac ttcccgatac cgacaacctc acagtgccct
     3121 tcgaagctgt taacgtgcac tctttgcagg ttacagtttt taaggtttac gacaaaaaca
     3181 tgggacagtt ctttcaggtt aacgacttta agcaaaacaa cgaaataaaa cgtgttggtc
     3241 gctacctgtg gcgtaaaaca atcaaactta acgctacacc ggcagaactc tctaaatggt
     3301 ccagatacag cctagacgta agctctcttt acaaggagca cccaggcagt ctatttcgta
     3361 tagaactttc aattaacaga tcaaattcgg ctctgggctg ctctgataat ccggatccgg
     3421 caattgtgga acccgacttt accaaccagg aagctacaat aataagtgac agcagttatt
     3481 gggactactg ggaagagtat gatcacagtg aatactggga caacagaaaa aacccatgcc
     3541 acgatgctta ttacaaacag tcttatggca atcccgtgtt tagtgcaaaa aatttcttat
     3601 catcaaatat tggaataaca gccaaatacg gatcaggcaa aacgctgacc gttgttacca
     3661 cagatattgt ggacgcgtct cctacgtcgg ctagtataaa aatatacaat tttcaaaata
     3721 ttgaaattgg ctccggctca acagacggaa atggattcgc ggacattacc gtatcgggag
     3781 aaggcgaccc gttttatatt gttgccgaaa aacgcgggca aaagggctac cttaagttaa
     3841 gtggagcgtc acagcttatt ataagccact ttgatgtagg cggtgaaaaa gtcaaaaatg
     3901 gattaaaagg gtatatttac ggagagcgcg gagtttggtc tcctggtgat aatatttacc
     3961 ttacctttgt cttggaggat aaagacaatc tgttaccaaa agatcacccg attaccatga
     4021 agctctatga cccacgcgga cgtctgaagc agattcaatc ggcttacaga caggctaata
     4081 atttttatgc ttttgagctt aagacagata gtgaagacga gaccggctca tggaccgcgc
     4141 gcgccgagct tggaggatta agttttacaa aaacattaaa aatagagacg gttaagccaa
     4201 accgattaaa ggtcgaactt gatataggtg ggaaagagct acaccgttca aaaatgcctg
     4261 ttagcgcaaa aataaattca caatggttac acggcgcaac tgctcaaaat cttacaacgg
     4321 atgttagtgc cagattttcg ggccttacta ctgaattttc acgctacaca gattatgtat
     4381 tcgatgattt aacacgtact gcttcgtctg aaaaaacaga gatattctct ggctcacttg
     4441 actctacagg caaagcctct tttaatatat cgtttagcga caatagcaaa cctgctggta
     4501 tgcttaatgc ctactttaca
//
LOCUS       DAOTGZ010000219         4511 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_3064
            NODE_22832_length_4511_cov_4.251346, whole genome shotgun sequence.
ACCESSION   DAOTGZ010000219 DAOTGZ010000000
VERSION     DAOTGZ010000219.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868088
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 4511)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4511)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:22:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,785
            CDSs (total)                      :: 2,751
            Genes (coding)                    :: 2,732
            CDSs (with protein)               :: 2,732
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 12 of 19
            Pseudo Genes (internal stop)      :: 5 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4511
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_22832_length_4511_cov_4.251346"
                     /isolate="AMR_MDS_3064"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..301
                     /locus_tag="P5123_11685"
     CDS             <1..301
                     /locus_tag="P5123_11685"
                     /inference="COORDINATES: protein motif:HMM:NF012626.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="phospho-sugar mutase"
                     /protein_id="HRX16965.1"
                     /translation="QVDGKDLETVKDYHKQTETNIITGKSSTLSDFPVSDVLQYHYTD
                     GSSFAIRPSGTEPKIKFYISAVTKTTEKDIHNSKESAENSVCNIMQYIQNRIDSL"
     gene            complement(338..796)
                     /gene="ybeY"
                     /locus_tag="P5123_11690"
     CDS             complement(338..796)
                     /gene="ybeY"
                     /locus_tag="P5123_11690"
                     /inference="COORDINATES: protein motif:HMM:TIGR00043.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004540 - ribonuclease activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA maturation RNase YbeY"
                     /protein_id="HRX16966.1"
                     /translation="MNLCQIEIMDNITIPFNGIDDSFISDVSVKIMDILELNNCVITV
                     ILCNNSEIQEINCNYRFKDYPTDVISFAYREESFPGEDSDSSEILGDIFLSLEKALEQ
                     SKELAHDLQTEITRLMVHSILHLIGYDHETGSDDEKIMFKKEDEVLEKLV"
     gene            complement(796..3006)
                     /locus_tag="P5123_11695"
     CDS             complement(796..3006)
                     /locus_tag="P5123_11695"
                     /inference="COORDINATES: protein motif:HMM:NF019317.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HDIG domain-containing protein"
                     /protein_id="HRX16967.1"
                     /translation="MRLSLLFVVFLVAITTIYLSISKFGITYEYQIGDIATENIRVPW
                     DITYLQKQETETEIERVMAAVPLVFDMNRSILLDRIQHINSIFSQIDAVVNSSDGKAI
                     NEDSVKLAIVRDGISTPDKYPDNVLLAFIRFKDIESLRITSIRIINDIYERGILEEEY
                     SNPLKINNRNTVVRTIGENNEYVEERRSLDTLHSLNSITKGLNALCSEYTSAFPKNQQ
                     WAIYLMVKENIVQNIRFNREETKRRLQQAADDVAPVTGMLKKGQVVVREGDSVTSDSL
                     RLISILNKYTSKFNIKYVAGIILFQIIFYFILTFFLKDYDRLIYFNSKSMAVVTILLF
                     AFFTYSFIAARTIDYLNTNMVFSLYLPITFVTMIVTILFSVPLAMITGIYILFFVMMI
                     TGADFSDLAITFSSGFLGIFAVKTIEKRVDFLKTGLLIGIANTMIVSALVFMNNIAEE
                     QFPGNIRLSLISGFVNCIIVMGVFPIFEHFFGLTTRFKLYELTDLNAPIFREMLIKAP
                     GTYNHSILVANMAEAACKKIGANSLLARVGSYYHDIGKLKNPHIFIENMNTKGAVNYA
                     PHVYCKKIINHVKEGVKLAKEHEIPQDVVAFIEEHHGNSVMTFFYHEALDLANKSKKK
                     NKPNVDIDDFRYPGPKPKTKETAIVMLADAIEAASRSVVKPTEEKLEQLVYKIISGRL
                     NDGSLDDADLNMKELSAVRDGFVSVLAGIFHTRMKYPDQKSIKNLEKKVNEHKK"
     gene            complement(3076..4041)
                     /locus_tag="P5123_11700"
     CDS             complement(3076..4041)
                     /locus_tag="P5123_11700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013052660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PhoH family protein"
                     /protein_id="HRX16968.1"
                     /translation="MSDFVFETDNRALYQKICGFKDQNLVSIGNALTTRLTPRGNSII
                     ISGSKEHCIIAEKMLHVMSDYLEDKPENYEFDRYDLKYLVTLAKSGEKINFAAINRLK
                     IYIPESKKSVYPKSLNQIHYLNEIDKKAVTFAIGPAGTGKTYLAVAAALKEYYNGNIR
                     RIVLTRPAVEAGESLGFLPGDLVQKINPYLRPLYDALFDLVSFEKIEVMIEKNIIEIA
                     PLAYMRGRTLNDAFIILDEAQNTTASQMKMFLTRLGNNSKMVISGDITQIDIEKPKRS
                     GLLLVRKILRKVNDVSFVEFNSEDICRHPVVEQIINAYDEFSGEH"
     gene            complement(4041..>4511)
                     /locus_tag="P5123_11705"
     CDS             complement(4041..>4511)
                     /locus_tag="P5123_11705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012674475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRX16969.1"
                     /translation="LFEYDEEDKRFYAVHHPFTAPVASDLAKLDNLKPEDVEEITSDA
                     YDIVLNGSEIGGGSIRINNIDLQKKIFTLLGISEEEAREKFSFLLEALKYGAPPHGGL
                     ALGLDRIVMLLLNRESIRDVIAFPKTQKGQCMMSEAPSPVDKKQLRELSLKIIE"
BASE COUNT         1410 a          879 c          783 g         1439 t
ORIGIN      
        1 tcaggtcgac ggtaaggatc ttgaaactgt taaggattat cataaacaaa ctgagaccaa
       61 cattattacc ggaaaaagct cgaccttaag tgacttccct gtatctgacg tacttcaata
      121 tcactatact gacggatcct ctttcgccat tcgtccatcc ggcactgaac ctaaaataaa
      181 gttttacata tcagctgtta caaagacaac agagaaagat atacataatt caaaagagag
      241 cgctgaaaac tcggtttgca acattatgca atatattcaa aacagaattg attctttgta
      301 aatattttag aaaaatacat gttcaatttt caaaaaatta gacaagcttt tccaaaacct
      361 catcctcttt tttgaacatg attttttcgt catctgatcc ggtttcatga tcgtatccaa
      421 taagatgcag tatagagtga accataagcc ttgtaatttc ggtttgtaaa tcgtgggcaa
      481 gctcttttga ctgttccaaa gccttttcaa gtgataaaaa aatatcgccg agtatctctg
      541 aagaatcact gtcttcaccg ggaaatgatt cttcgcggta tgcaaatgat ataacatcgg
      601 taggatagtc tttgaagcga tagttacaat ttatttcttg tatctccgaa ttattacaga
      661 gaataacagt tataacgcaa ttattcagtt ccagaatatc catgatttta acggaaacgt
      721 cactgataaa actatcatca ataccattaa aaggaatcgt gatattatcc atgatttcta
      781 tctggcacaa attcatcact tcttatgctc gttaactttt ttttccagat tttttatact
      841 cttttggtca ggatatttca tccttgtatg gaatattccc gcgagaacag agacaaaacc
      901 atctcgtaca gcgcttaact ctttcatgtt cagatctgca tcatcaagag aaccgtcatt
      961 taaacgaccg gaaataattt tataaacaag ctgttcgagc ttttcttctg taggcttaac
     1021 aactgatctt gaagcagcct ctatagcatc ggccagcata actatagctg tttccttggt
     1081 tttgggtttt ggtcccgggt atctaaaatc atcaatatcg acattcggtt tattcttctt
     1141 tttactcttg ttagccagat caagtgcttc atgataaaaa aatgtcataa cggaattgcc
     1201 gtgatgctct tctataaacg cgacaacgtc ctgaggaatt tcgtgctctt tcgctagttt
     1261 caccccctct ttcacatgat ttataatttt tttacagtaa acgtgaggag cataattgac
     1321 tgcccctttt gtattcatat tctcaataaa aatatgagga tttttcagtt tcccaatatc
     1381 atgataatag ctacccactc ttgctaataa cgaatttgct ccgattttct tacaagctgc
     1441 ttctgccata tttgcaacaa gtatcgagtg attatatgtt cctggagcct tgattaacat
     1501 ctccctgaaa ataggtgcgt ttaaatctgt aagttcataa agcttaaacc ttgtagttaa
     1561 gccgaaaaaa tgttcaaata tggggaaaac acccattaca atgatacagt taacaaagcc
     1621 agaaattaaa gagaggcgta tattgcctgg aaactgctcc tctgctatat tgttcataaa
     1681 aaccagagca gaaacaatca ttgtattggc gataccgata aggagtccgg tttttaaaaa
     1741 atccacccgt ttttctattg tcttcaccgc aaaaattcca agaaaacccg aactgaatgt
     1801 tatggcaaga tcagaaaaat cggcacctgt aatcatcata acaaagaaaa gaatataaat
     1861 ccccgttatc attgccagtg gtacagagaa tagaattgta acaatcatgg ttacaaaagt
     1921 tattggaaga tataaagaaa acaccatatt agtattaagg taatctatag ttcttgctgc
     1981 aataaacgaa taggtgaaaa aggcaaaaag aagtatcgta acaacggcca ttgattttga
     2041 attgaaatag ataagcctgt catagtcctt caaaaaaaac gttagtataa aataaaatat
     2101 gatctggaaa agaattatcc ctgcaacata ctttatgtta aatttagatg tatatttatt
     2161 tagtatactt attaatctta aagagtctga ggtcacagag tcgccttccc taacaacgac
     2221 ctgccccttc ttaagcatac ctgtaactgg agcgacatcg tcggcagcct gctgaagtct
     2281 tcgttttgtc tcttccctgt tgaatctgat attttgaact atgttttctt taaccataag
     2341 atatatggcc cattgctgat ttttgggaaa tgcactagta tattcggaac ataaggcatt
     2401 aagccccttt gtaatagaat tcaaagaatg cagggtatca agagaacgcc gctcttccac
     2461 atattcgttg ttttcgccaa tagtacgtac taccgtgttt cgattattaa tttttagagg
     2521 gttgctgtac tcctcttcga gtataccgcg ttcataaata tcatttatta ttctgatcga
     2581 ggtaatgcgt aacgattcta tatctttgaa tcgtataaaa gcgagaagca cattatcagg
     2641 atacttgtcc ggagttgata ttccgtctct tacaatagcg agtttaacac tgtcttcatt
     2701 gatagctttc ccgtcagaac tgttaacaac agcatcgatc tgagaaaaaa tagaattaat
     2761 atgctgtatg cgatctagca gaatactgcg gttcatatcg aaaaccagcg gaaccgcggc
     2821 cataaccctt tctatctcgg tttcagtctc ctgcttttga agatatgtga tatcccaagg
     2881 cacacgaata ttttccgtag cgatatcgcc tatctgatac tcataggtaa tgccaaattt
     2941 actaatagaa agataaatcg tggtaattgc gacaagaaaa actacaaata gaagagataa
     3001 tcgcatattt ctgtctttga aagtatagaa gaactggata aaagaacgtg tcaataatga
     3061 tttcattatt agattttaat gttctccgga aaattcgtca tatgcattga ttatctgttc
     3121 gacaacagga tgtcggcaga tatcctctga gttgaactct acgaagctga catcatttac
     3181 cttccgtagt atttttctta caagcagaag tccggaacgc ttgggttttt ctatatcaat
     3241 ctgggtaata tcaccagata ttaccatttt agaattgttt ccaagtctgg tcaaaaacat
     3301 tttcatttgt gaagcagtag tattttgagc ttcatcaagt attataaaag cgtcattaag
     3361 ggttcgccct cgcatatatg ccaagggagc aatctcaata atgttttttt cgatcattac
     3421 ttcgattttt tcgaacgaaa caaggtcaaa aagtgcatca tatagcggtc taagataagg
     3481 gtttatcttc tgaacaagat ctccgggaag aaaccccagg ctttcacctg cctcaactgc
     3541 cggtcttgtc aaaacaatac gccttatatt gccattataa tactctttta atgccgcggc
     3601 aaccgcaaga tatgtttttc cggttccagc aggcccaatt gcaaaagtta cggctttttt
     3661 gtctatttca ttgagatagt gtatctgatt aagagacttg gggtaaacac ttttttttga
     3721 ttcaggtata taaattttta atcggttaat cgcggcaaag ttaatttttt caccgctttt
     3781 tgcgagagtt acaagatatt ttagatcata tctatcaaat tcgtagtttt caggtttgtc
     3841 ttcaaggtag tcggacatta catgaagcat tttttcggca ataatgcaat gctctttaga
     3901 ccccgagata ataattgaat ttccccgggg ggttaacctc gtcgttaaag cattacctat
     3961 tgaaacaaga ttctgatcct taaaaccgca aattttttga tataacgcac ggttatctgt
     4021 ttcaaagaca aaatcagaca ttattcgata atcttgagtg aaagttcacg taactgcttt
     4081 ttatcaaccg gagaaggagc ctcactcatc atgcactggc ctttctgtgt tttagggaat
     4141 gcgataacat cgcgaataga ttcgcgatta agtaaaagca ttacaatacg gtcaagccct
     4201 aatgctaaac ctccgtgagg aggagcacca tattttagtg cttccagcag aaacgagaat
     4261 ttttcacggg cttcctcttc agatattccc aaaagtgtga atattttttt ctgaagatca
     4321 atattgttga tccttataga tcctccccca atttcacttc cgttaagaac aatatcgtaa
     4381 gcatcagaag ttatctcttc tacatcctct ggtttaagat tatccagctt ggcaaggtca
     4441 gacgcgaccg gagccgtaaa tgggtggtga accgcgtaaa atcgtttatc ttcttcatca
     4501 tattcaaaca a
//
LOCUS       DAOTGZ010000220         4495 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_3064
            NODE_22927_length_4495_cov_5.183784, whole genome shotgun sequence.
ACCESSION   DAOTGZ010000220 DAOTGZ010000000
VERSION     DAOTGZ010000220.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868088
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 4495)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4495)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:22:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,785
            CDSs (total)                      :: 2,751
            Genes (coding)                    :: 2,732
            CDSs (with protein)               :: 2,732
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 12 of 19
            Pseudo Genes (internal stop)      :: 5 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4495
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_22927_length_4495_cov_5.183784"
                     /isolate="AMR_MDS_3064"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..368)
                     /locus_tag="P5123_11710"
     CDS             complement(<1..368)
                     /locus_tag="P5123_11710"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRX16970.1"
                     /translation="MRNFIFASLMIVLVSCGTTQKKTDTPADSVITKIDNINTKPEFI
                     RANVSVNITMERMSSLNVSGVAYLQNEPLKVRVNLIEPIFKSTVMDLLADDDRVVVLF
                     PIDQKAQIIKGGLTKESLNGV"
     gene            complement(478..2328)
                     /locus_tag="P5123_11715"
     CDS             complement(478..2328)
                     /locus_tag="P5123_11715"
                     /inference="COORDINATES: protein motif:HMM:NF024579.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRX16971.1"
                     /translation="MKRFLILFFLITIFASSALYSSDTSVSRDYSRALHLITEKKYKE
                     ALVLLVDLEGKDGVSKHHVLYQRALCHYYLGESDLAIDYSKKSLKEKRNFEKPYLLLF
                     DLYNEKNNFDDSTYYLHTLVESVPQQYEHYFTLGIMYGKYIKNNELSKYCFEKVIKIS
                     KKMPVPSNYLENSYLYLSSIYFEEEQNEKAINALNMAVKYNPDHNSRFYNVANYYISK
                     NMLHEAVLSIRFFTDNLTDEQKKEAFIYKVYAFLGRMYYLQDKKEAMLYLRLGAIDSS
                     FDGQICRALFEHLAGNTEKAEKLLLEIVKKNNGYISPYYALAKIMDKNGKKAEAHDYY
                     ITTALMLYNSGLTMSAKTSLLGAIAIKPDQPKTHELLARLYESEKKYASALYHYKKSD
                     PNNEDIEKLIHTSFLYFYINDYKGALKTIDKAMAVDAKNDRVLFISGIVNHDVNNYKK
                     SIEHFERALQINESKAEYYYYMAMSQEKLELYDESVASLKIAINLDKNNPDYYNFLGY
                     LYAERNTNLDESYNLLSEALRLRPFNGAYLDSMGWLYYQKGEYELAERYLVRAYRNLM
                     AIGQRDTVVYDHLGDTYEKMGNKRLAIKFWKRALSLDKTNEKIKQKIENR"
     gene            complement(2525..3988)
                     /locus_tag="P5123_11720"
     CDS             complement(2525..3988)
                     /locus_tag="P5123_11720"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009975 - cyclase activity [Evidence IEA];
                     GO_process: GO:0009966 - regulation of signal transduction
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HRX16972.1"
                     /translation="MDRSIYINPVSDKFLSSDVRDILSELGVFVRSNITYPFIPNNIW
                     YQCGYSDNELSDDSWTTFVHPEERDRVFHSWDSFVKGEKLSFDEIYRFKTKNGKWIWL
                     HNRCTSIERDENGSLLLYAGIDNDITHHKATENELRKAINLADRRVVESEMVRSIVAL
                     VSGSLKVAETAELVLSHIKTLVNYQRAMILIVEDSLIRIVDCAGFDNREKLSKMVFDY
                     PDPHNPVCMTVQNGQALALNFGDIKYADFFKKTENALTSWICVPLKSNEKIIGALVLE
                     HEEKDFFSRRNIELISALSEHIAISIDNAILHENMYNLAMIDSLMKIGSRHNFNYKAK
                     LVFSQALRYQRALTTLLIDVDLFKSVNDTYGHDTGDMVLRKIAESCSGVLRDSDIIAR
                     YGGEEIIVLLPETQCCMGANVAERIRRSVQSGTYEKVKKAVTVSIGLYSVVPDKTVTL
                     ESCIQKADEALYTAKENGRNRVEVHSESMITDSDIIS"
BASE COUNT         1407 a          850 c          693 g         1545 t
ORIGIN      
        1 actccattca aactctcttt ggttagccct cctttaataa tttgagcttt ctgatctatc
       61 gggaacagaa caactactct atcatcatct gctaaaagat ccattacagt actcttgaaa
      121 attggttcaa ttaaattaac ccttaccttt agcggctcat tttgcaaata tgcaacacca
      181 gaaacattta aactgctcat tcgttccatt gtaatgttta cagaaacatt cgcgcgtata
      241 aattcaggtt ttgtatttat attatctatc ttggtaataa cagaatcggc aggagtatct
      301 gttttttttt gtgtagtgcc gcatgaaacc aacactatca ttaaagaggc aaatataaaa
      361 tttctcatta tctatttcct tataaaaaat tagcaattta tgttcagttc acatccattg
      421 ttacataaag catgatagta aattatcatt atactgataa cgtcatttat ggcataatca
      481 tctattctct atcttttgtt taattttttc gtttgtcttg tctagcgaca atgctctttt
      541 ccagaatttg atagccagtc ttttgttacc catcttctcg tacgtgtctc caagatgatc
      601 gtatacaaca gtatcgcgct gaccaatggc cattaggttc ctatatgccc ttacaagata
      661 acgttcggca agttcgtatt cacctttttg ataatacaac cagcccattg aatcgagata
      721 ggctccatta aaaggtcgca accttagagc ttcacttaaa agattatatg attcgtcaag
      781 attagtgttt cgctcagcat aaagatatcc taaaaaatta taataatctg ggttattctt
      841 atccaggttt atcgcgattt tcagcgatgc tacagattca tcatacaatt cgagtttttc
      901 ctgactcatt gccatatagt aatagtactc ggctttgctc tcgtttatct gtaaagctct
      961 ctcaaaatgc tctatagatt ttttataatt attaacatcg tgattaacta ttccagagat
     1021 aaaaagaact ctgtcatttt tggcatcgac cgccattgcc ttatcaattg tttttaaagc
     1081 ccctttgtaa tcgttaatgt aaaaatataa aaaggaagta tgaataagct tctcaatatc
     1141 ttcattattt ggatcagatt ttttatagtg ataaagggca gacgcatatt tcttttcgga
     1201 ttcatagagt cgagcaagaa gctcgtgggt cttgggctgg tcaggtttta tagcgattgc
     1261 tccgagcaat gaagttttag cactcattgt cagtccggaa ttataaagca ttagtgcggt
     1321 tgtaatataa tagtcatgtg cttcggcttt tttgccattt ttatccatta tcttggcaag
     1381 agcataatat ggacttatgt atccattatt ttttttgaca atttcaagaa gcaatttttc
     1441 ggctttttct gtgttaccgg ctaaatgttc aaataacgca cggcatatct gcccatcaaa
     1501 gctagaatct attgctccaa gccgaaggta taacattgcc tcttttttat cctgaagata
     1561 gtacattcgc cccagaaaag cataaacctt ataaataaaa gcctctttct tctgttcatc
     1621 tgttaaatta tcagtaaaaa aacgaataga aagaacagcc tcatgcaaca tgtttttaga
     1681 tatatagtag ttagccacat tataaaacct gctattatga tcagggttat atttaacagc
     1741 catatttaaa gcattgatag ccttttcgtt ttgctcctct tcaaaatata tgcttgaaag
     1801 atatagatag ctgttctcta agtaatttga cggtacaggc attttttttg atatttttat
     1861 aactttctca aaacaatatt ttgaaagttc attatttttt atatatttac cgtacattat
     1921 tccaagcgtg aaataatgct catactgctg cggtacagat tcgacaagtg tatgaagata
     1981 atatgtcgaa tcatcaaagt tgtttttttc gttataaaga tcaaaaagaa gcaggtatgg
     2041 tttctcaaaa tttcgttttt ctttaaggga tttttttgag taatcaatcg caagatcgga
     2101 ttcacccaga taatagtgac acaaggctct ttgatacaaa acatgatgtt tggaaactcc
     2161 atccttacct tccagatcga ccaacaacac aagggcttcc ttatattttt tttcggttat
     2221 taaatgaagt gccctgctgt aatctcgtga aacagaagta tcagaagaat agagagcgct
     2281 gcttgcaaat attgtaatta aaaaaaatag gattaaaaaa cgtttcatat tgtacctgta
     2341 atctatgttt tattgtaagt ctattactag tgttttctcc gtgtaaaaaa gcacaaatta
     2401 aaaagaacat acaagttttt cccgcgccgc tgacgcgata aaacttatta ttttatatag
     2461 ggaacttcaa aaaattacca taaaataaag ctaaaacgaa ttattaattt gcagtgattt
     2521 tttatcaaga aataatatca gaatcagtta tcattgattc agaatgcacc tctaccctgt
     2581 ttcgcccgtt ttctttcgca gtatacaagg cttcgtcagc cttctgtatg caactttcaa
     2641 gcgtaactgt cttatccgga acaacggaat agagaccaat acttacagtc acagctttct
     2701 ttactttttc atacgttccc gactgtacac tgcgccgaat gcgctcagcc acattcgccc
     2761 ccatacaaca ttgtgtttct ggcaaaagga caataatctc ttcaccaccg tatcttgcaa
     2821 ttatatctga gtcacgcaaa acaccagaac aactttccgc tattttacgt aataccatgt
     2881 caccagtatc atgtccataa gtatcattta cacttttaaa aagatcaaca tcaattaaaa
     2941 gcgtagtcaa tgccctttgg tagcgcaaag cctgagaaaa aacaagtttt gccttatagt
     3001 taaaattatg tcggctacca attttcatta atgaatcaat catagcaaga ttgtacatgt
     3061 tttcatgaag tatcgcatta tcgatagata tagctatatg ttctgataaa gcagatataa
     3121 gctcaatatt acgtctgcta aaaaaatctt tctcttcatg ctctagtaca agcgcaccaa
     3181 taatcttctc attactcttt agaggaacac aaatccatga agttaaagca ttttctgttt
     3241 ttttaaaaaa atctgcgtat ttaatatctc caaaatttaa agcaagtgcc tgaccgtttt
     3301 gaacagtcat gcacacagga ttatgtggat caggataatc gaaaaccatt tttgaaagtt
     3361 tttccctatt atcaaagccg gcacagtcca caatacgaat taaagaatct tcaacaatca
     3421 gaatcattgc gcgttgatag ttaacaagag tttttatatg actgagcacc aattctgccg
     3481 tttcggcaac ttttagactg ccagagacaa gtgccactat ggaacgtacc atctctgact
     3541 caacaacacg tcgatcagcc aaatttatag cctttctaag ttcgttttct gttgctttgt
     3601 gatgtgttat atcgttatca attccggcat aaagcagtaa tgagccgttt tcgtcccttt
     3661 ctatggaagt gcaacggtta tgtagccata tccatttacc attttttgtt ttaaaacgat
     3721 atatttcatc aaaagagagc ttttcacctt ttacaaaaga atcccatgag tgaaagaccc
     3781 tatctctttc ttcggggtgt acaaaagtag tccacgagtc atcagatagc tcattatcag
     3841 aataaccaca ctgataccat atattattgg gaataaatgg atatgttatg tttgaacgga
     3901 caaaaacccc aagttcagag agaatatccc ttacatctga ggacaaaaac ttgtccgaca
     3961 ccggattaat atatatagaa cgatccataa tagctccatt ttttcaaata aactcacctt
     4021 ttattatctt ttatacaatc taatgtcaag cctgatttag ctggttaatg tagactattt
     4081 gtgcacttcg caatttttat ttatacataa acgtctatta attcatagtt ataatataaa
     4141 ggttattaat gatgaaaaga tgagttgact ttttttattt gcagcaatct gattgatgtc
     4201 atggtttatt cggtcatgga aatcgattat taaaagtata ttttcttttt gctttaattc
     4261 ataatttcat caatattggg ctattcccaa aaaagttgat tattaaaggc tatctatatt
     4321 atttggagat aactatggaa cagatcaagg gaacaaaact ctttctttat aacttgtcat
     4381 ccgctggaca cgctcttttt gattcaattc ttctcgcttt ttatatgaac tttttgttgc
     4441 cgccttcaga aacggtttcc tctggcgaac ttgaacaata cctacctgag aagat
//
LOCUS       DAOTGZ010000221         4462 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_3064
            NODE_23165_length_4462_cov_5.034264, whole genome shotgun sequence.
ACCESSION   DAOTGZ010000221 DAOTGZ010000000
VERSION     DAOTGZ010000221.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868088
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 4462)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4462)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:22:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,785
            CDSs (total)                      :: 2,751
            Genes (coding)                    :: 2,732
            CDSs (with protein)               :: 2,732
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 12 of 19
            Pseudo Genes (internal stop)      :: 5 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4462
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_23165_length_4462_cov_5.034264"
                     /isolate="AMR_MDS_3064"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1468)
                     /locus_tag="P5123_11725"
     CDS             complement(<1..1468)
                     /locus_tag="P5123_11725"
                     /inference="COORDINATES: protein motif:HMM:NF013069.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HRX16973.1"
                     /translation="MQRLVTFFISHRMFVNILVLTVFIGGAVSAYNSRKEGFPEVSMN
                     RITIETRYPGAAVSDVELNVTVPIENELLTIDGIKEVLSVSEESLSIVEVRADDNSTD
                     RDFAKLFDDIDRAVQSSLDLPDNVEGRPVIREFTSSDVPVLEIGYSGEYEKLKPYIDQ
                     LEVKLRRIPGVGEISIAGVPDMEVQILIDPVKAKSYNVDLRMISAAVGKRNLEGSGGT
                     LESFVGERKVVLFSKFDDYREVLNTNVIMNRDGYGIKLGQMAEVIMVPENRNLIVRNN
                     GRRGASITIKKTGSSDLIKTVDSVYELLNAEVIPDGVDMKILFDQSRLTRNRISLLAG
                     NALMGFALVIIILIGIFNYRTALWTAFGIPFTLFALFIFMKAIGLSINLISLGGFIII
                     IGMLVDDAIVISEETNTNREGGLSPTDAAKMAVKDMWMPVLASSLTTMIAFSPLFFLN
                     GFPGKFIWAIPLMVVVGLAISLFESFFVLRFVLFFQFSR"
     gene            complement(1882..2223)
                     /locus_tag="P5123_11730"
     CDS             complement(1882..2223)
                     /locus_tag="P5123_11730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008512624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HRX16974.1"
                     /translation="MIIRKVDDVPVMETAHNVDARNIYNTENAMVTVIRLKPGQSLKK
                     HITPVDVAFFVLQGEGVVEVGEERAVAGKDSLVESPKDISHCWYNESSEDLVFMVIKA
                     PRPAKKTVFVS"
     gene            2443..2751
                     /locus_tag="P5123_11735"
     CDS             2443..2751
                     /locus_tag="P5123_11735"
                     /inference="COORDINATES: protein motif:HMM:NF021886.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRX16975.1"
                     /translation="MPVTIYRLELPPIRHKLLVQLSWSDRLKTEDIIKLLETYAKQIQ
                     NRLDIYNSTQQTSIAELGRTKRERLLWRALQDNGITAYNAELDWVRRTIEKLKKHELM
                     "
     gene            2757..3128
                     /locus_tag="P5123_11740"
     CDS             2757..3128
                     /locus_tag="P5123_11740"
                     /inference="COORDINATES: protein motif:HMM:NF012730.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005267 - potassium channel activity
                     [Evidence IEA];
                     GO_process: GO:0034220 - monoatomic ion transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="potassium channel family protein"
                     /protein_id="HRX16976.1"
                     /translation="MRKIQDLLKDKKEELILSFVFVFFLLLFTSTLMYYVEHTYQPDK
                     FKSIPESMWWSISTLTTVGYGDIYPISPEGKILGGFISLIGIAIFALPAGILASGFSE
                     NRDKKKITRCPHCGKKIDLKS"
     gene            3332..>4462
                     /locus_tag="P5123_11745"
     CDS             3332..>4462
                     /locus_tag="P5123_11745"
                     /inference="COORDINATES: protein motif:HMM:NF024140.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MCP four helix bundle domain-containing protein"
                     /protein_id="HRX16977.1"
                     /translation="MSFLNNLKMSFRLFLVFLCIILITGAGMVYNLKNLREISGIINS
                     IYNTRLKSINFLIEADRDAYQSSIAVSTSFAEQINSNPELIKKNLDEISENMIQVNER
                     FGKFRDIFLKSESDSHIHDFAYFKTQYGKWEAFSNTVSAHIKNGEYNNAQNIYFGEYW
                     TAFNEMRNTMDKLTEVSLKSAEDEYNTSIKQIENIQKASILMIGITMFFCIFMGVVLT
                     ISILGPIKKVITAMQGVAKGDLTVEIEALGRDELSTMMKTLKETVLSLNTIVSHIHSS
                     SQTITTYADEVNATALSLSESTNEQAANVEEISSSLEEIGSTISMNTENSKNTDSLAQ
                     GVSANAEEGGKAVIQTVDAMKSIAEKIGIIEDIAYQTNLLALN"
BASE COUNT         1487 a          951 c          770 g         1254 t
ORIGIN      
        1 ttcgagaaaa ttgaaaaaat aacacaaaac gaagaacaaa aaaactttca aaaagagaaa
       61 ttgcaagccc caccacaacc atcagaggaa tggcccagat aaattttccg ggaaacccgt
      121 taaggaaaaa caggggggaa aatgcaatca ttgtagtcaa tgatgaagca agaaccggca
      181 tccacatatc tttgactgcc attttagcgg catcggtcgg cgaaagtccc ccctccctgt
      241 ttgtgtttgt ctcttctgaa ataactatag catcatctac aagcatacca attattatta
      301 taaaccctcc aagtgaaata agatttatag acaaaccaat ggctttcata aaaataaaca
      361 gggcaaacag ggtaaaaggt attccaaaag cagtccaaag agctgttctg taattaaaga
      421 taccgattag tataattatt accagagcaa aacccataag ggcattccct gctagaagag
      481 aaatacgatt acgtgttaat cgggattgat caaacagaat tttcatatca acgccgtcag
      541 ggattacttc ggcattcaac agctcatata ctgaatcaac agttttaatt agatctgaac
      601 ttcctgtttt ctttattgtt atagacgcac cgcgtcgccc gttgttacga actatcaggt
      661 ttcgattctc aggtaccata ataacttcag ccatctggcc aagctttatg ccatacccgt
      721 cacggttcat aattacattt gtattaagaa cctcacgata atcatcaaac ttgctaaaca
      781 gcacaacctt gcgctcacca acaaacgatt ccagtgtgcc acccgagcct tccaggttac
      841 gttttccaac agcagcagaa atcattcgca aatcaacatt gtagctctta gctttaaccg
      901 gatcaattaa aatctgcacc tccatatccg gcactccagc gatgcttatc tcacccactc
      961 ccgggattcg cctgagtttt acttcaagct ggtcaatata gggctttaat ttctcatatt
     1021 ctccggaata tcctatctct aatacaggaa catccgaaga tgtaaattca cgtattacag
     1081 ggcggccttc tacattgtcc gggagatcta aagatgattg aacagcacga tcaatatcat
     1141 caaaaagttt cgcgaaatcc cgatcggtag aattatcatc agctctcact tctacaatag
     1201 agaggctctc ctctgataca gaaagaacct ctttaattcc gtcaatcgtc agtagttcat
     1261 tttcaattgg tactgttaca tttagctcga catcactaac agcggcaccc ggatagcggg
     1321 tttcgatcgt aatgcgattc atggaaacct cgggaaaccc ctctttacgg gagttgtaag
     1381 cggatacagc gcctccaatg aatacggtta aaacaagaat gtttacaaac attctgtgag
     1441 aaataaaaaa agtcaccaat cgctgcatcc taaccttcct ataaactaaa aaaaatcgca
     1501 taaaaagccg acagtgagcc gtcattaaaa atatatgtca gcgtttttga gccttcgcga
     1561 gtaaaattat acataataaa acaaaacatg cccaatagtc tgtaatatta cgtaagccaa
     1621 gaaattgtta ttcttgtaat tatattatct gcaagtatcc tcaaataagc caattaatct
     1681 cgcaattatg gagtataaac tagagggaac ttcgaaaaat taccatttct ggagcggcta
     1741 tgccgtttaa agaccatata taaaggaatt tttcgcaagt tccttgttaa aaagttaatt
     1801 attagagatt gcctagatac tattcacgtt tataaccaca aaaattttta atctttgaaa
     1861 aataacgctt ttaaggcata atcaacttac aaaaacggtt ttcttcgccg gacgtggtgc
     1921 tttaataacc ataaatacca gatcctcact gctctcgttg taccagcagt gcgaaatatc
     1981 cttggggctc tctacaagac tatccttgcc agccacagct ctctcttcac ctacctccac
     2041 tacgccttca ccttgcaaaa caaaaaaggc aacatcaacc ggagttatat gtttcttcaa
     2101 agactgtccg ggtttcagcc ggattacagt taccatcgca ttctcggtat tgtaaatatt
     2161 cctcgcgtcc acattatgtg ccgtttccat aaccggtaca tcgtctactt ttcttataat
     2221 catattattt ctcctctatt tttattaaat caccttcaaa atatttcgct attcagaaat
     2281 ttcaatgata gtcattatta atacatatca catcatttac cgtataaaat tgaccaaggt
     2341 caactaaaca gattaaaata caactggcca atttatcatc acgcatatct ctgtgatatt
     2401 attcaaacaa agtaacatta ttaaatccag aatctggaat acttgccagt cacaatatac
     2461 aggttagaac ttccccctat tcgtcacaaa ttgcttgtac aactatcatg gtctgacagg
     2521 ctaaaaacag aagatataat caagcttctt gaaacctatg ccaaacaaat tcaaaacaga
     2581 ttggatatct acaacagcac ccaacagacc agtatagccg agctaggtcg tacgaaaaga
     2641 gagcgtctgc tttggcgcgc tttacaagat aacggtatta ctgcttataa tgcagaactt
     2701 gactgggtgc gaagaacaat tgaaaaacta aaaaaacatg aacttatgta aaagctttgc
     2761 gcaaaattca ggatttactc aaagataaaa aagaggagct tatcctctct ttcgtttttg
     2821 tctttttttt acttctcttc acttctactc ttatgtatta tgtagagcat acttatcaac
     2881 cggacaagtt taaaagcata ccggagtcta tgtggtggag tatttcgacg cttacaactg
     2941 ttggctacgg tgacatttac cctatcagcc ctgaagggaa aatattgggg ggattcattt
     3001 ctctaatcgg aatagcaata tttgcattac ccgcgggcat tctggcttcc ggtttttcag
     3061 aaaacagaga caaaaaaaag ataacaagat gcccacattg cggcaaaaag atcgatttaa
     3121 aatcttagcc ggttgtaact ggtatctgaa gctccgttag ccactcattt tctctatgcg
     3181 acttgttcca tatgccgtca atatatgact cccgcggatt acctgccata atcaagtggg
     3241 cactctcctg ttcggggaga gtcaacaaaa aatttgatca tttattatcc ttttattact
     3301 attagataac cttatgtcag gaggctcaat tatgagtttt ttaaataatc ttaagatgtc
     3361 tttcagactt tttctggttt tcttgtgcat aatcctgatt acaggtgccg gcatggtcta
     3421 caatctcaaa aatcttcgcg aaatatccgg catcattaat tcgatataca acaccaggtt
     3481 aaaaagcata aattttttaa tagaggcgga ccgtgatgct tatcaatcga gtatcgccgt
     3541 cagcacatct tttgccgaac agataaactc taatcccgag ctgattaaaa aaaatcttga
     3601 tgaaataagt gaaaacatga ttcaagtgaa tgaacgtttt ggcaaattca gggatatatt
     3661 tctaaaatct gagtctgatt cgcacataca cgattttgcc tattttaaaa cacagtacgg
     3721 aaaatgggag gcgttcagta acacagtatc agctcatatt aaaaatggtg agtataataa
     3781 tgcgcagaat atctattttg gtgaatattg gaccgctttc aacgaaatgc gcaataccat
     3841 ggacaagctg accgaagttt cacttaaaag tgccgaagat gaatacaata caagcataaa
     3901 gcagatagaa aacatacaaa aggcctctat attgatgatt ggcattacca tgttcttctg
     3961 cattttcatg ggagttgttt tgacaataag catactggga ccaataaaaa aagtaattac
     4021 cgcaatgcag ggagtggcaa agggagacct tactgtcgaa atagaagctc tcggcagaga
     4081 cgaacttagc acaatgatga aaacccttaa agaaactgtt ttatcattaa atacaattgt
     4141 ttcacacatt cacagctctt ctcaaactat tacaacatac gcggatgaag taaacgctac
     4201 tgccctttca ttaagcgaat caacaaacga gcaggctgca aatgtcgaag agatctcttc
     4261 ctctctcgaa gagattggat ccacaataag tatgaacacc gagaattcaa agaatacgga
     4321 ctctctggca caaggagtat cagctaatgc cgaagaaggg ggaaaggcgg taatacaaac
     4381 tgttgacgca atgaagtcaa ttgcggaaaa aatcggtatt attgaagata tcgcctatca
     4441 gacaaatctt ctcgccttaa ac
//
LOCUS       DAOTGZ010000222         4437 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_3064
            NODE_23317_length_4437_cov_5.477179, whole genome shotgun sequence.
ACCESSION   DAOTGZ010000222 DAOTGZ010000000
VERSION     DAOTGZ010000222.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868088
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 4437)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4437)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:22:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,785
            CDSs (total)                      :: 2,751
            Genes (coding)                    :: 2,732
            CDSs (with protein)               :: 2,732
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 12 of 19
            Pseudo Genes (internal stop)      :: 5 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4437
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_23317_length_4437_cov_5.477179"
                     /isolate="AMR_MDS_3064"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            267..953
                     /locus_tag="P5123_11750"
     CDS             267..953
                     /locus_tag="P5123_11750"
                     /inference="COORDINATES: protein motif:HMM:NF013786.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0008897 - holo-[acyl-carrier-protein]
                     synthase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4'-phosphopantetheinyl transferase superfamily
                     protein"
                     /protein_id="HRX16978.1"
                     /translation="MFQEITIKTIKIRVLNIMNFAKINFAELANNYLTSPPHVFKKDR
                     KIIEIFTDTELETINNFKALKKQIEWISGRVCIKTVASEILYTDPTKITISYEKYGAP
                     FIDLFPETSFSISHSGDFAGCAINTQTQSPVGLDIEQLKPIDYPGLSNITDVSFTDKE
                     KGTITDSESLFKIWTIKEAYLKYCRTGFHDTIKNVEFLNNSIMYKNQIVNINHHTQYI
                     DDSHIFSLVF"
     gene            957..2345
                     /locus_tag="P5123_11755"
     CDS             957..2345
                     /locus_tag="P5123_11755"
                     /EC_number="4.1.99.3"
                     /inference="COORDINATES: protein motif:HMM:NF013071.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribodipyrimidine photo-lyase"
                     /protein_id="HRX16979.1"
                     /translation="MANYKTTSSPLHSPETHLDNRTRLLSNGTQGEGVVIYWMERELR
                     ISDNHTLEAAQHHALLHNKKLQIIFIVHNYGTERQARHYRFALQGLLETIDEANRYGL
                     PFSVIVGDPVKEIVNLCTKYKCCALFTDFNPLRHIRLTKKLICEELPMPVFETDARNI
                     IPCFAASPKQEYSAHTLRLKHNRLIDDYHTPLPKIKKSELSKANPHPDIEKIIRDLHL
                     DSKGNYDHPFIPGAKEAKKLLKLFLTKKIHDYSTLDRDPNLDVISNLSPYIHFGQIGT
                     HRILSELIKTEISDDNSDKFYDQLVIRRELSENFVFYNDNYDNYNGIPDWGKRTLLAH
                     RVDPRDYNYRFDQFEAAVTHDELWNAAQKQMIQTGKMHCYLRMYWAKKILEWSASPEE
                     AFNTAITLNDRYSLDGEDPNGYAGVAWSIGGLHDRPWSERPVFGYVRFMNLAGCKRKF
                     NVDKFVKRFMEP"
     gene            complement(2513..3391)
                     /locus_tag="P5123_11760"
     CDS             complement(2513..3391)
                     /locus_tag="P5123_11760"
                     /inference="COORDINATES: protein motif:HMM:NF027232.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YCF48-related protein"
                     /protein_id="HRX16980.1"
                     /translation="MNKIISIVLSSIALLVFALQLGCGKKSNWEKCVISEIDSAQTNV
                     AFISKTEAFSAGYDGATHYSIDGGKSWKNGANETVCRYAIDVMPDGKFVHAGRGGTVG
                     ISNDKGKTWSILPTLLSDNVTLVSFSSEQEGYVVTKKSEIYNTKDGGVSWNPVKKPAS
                     SKFLAIKSNNENEICLVDNKGNFLYSNNNGENWETKPIISVEKCSFIEDYESCMASIQ
                     YADNNIKVAVLMPSSENNDGGVLIVSTSNNFESMKSEVIRSDGVSFMSKVFIENSGQL
                     ITVNNGNNAEAYRNKM"
     gene            complement(3565..>4437)
                     /locus_tag="P5123_11765"
     CDS             complement(3565..>4437)
                     /locus_tag="P5123_11765"
                     /inference="COORDINATES: protein motif:HMM:NF024242.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HRX16981.1"
                     /translation="KVLCDYELYLFIEKNSDLLLNHGIDDYYRLIFFQEGNGSISVNG
                     RVLPFISPALLCLTEKEKITGADSDKLRACNVIFKPDIINSKLTIDILNTKDNNYTST
                     ERMDLFCLRLFLNKAGKRSVIQLGPNSEVRIKDLLSSMYEILDNPVDDYWPCRARSYF
                     LELLNYSNRLDPLTMDKSVTKDDEINKIIAYLYTNYKEKITINEITEKFCIDRTTLSK
                     RFQKASGQTIIEYLNRLRINIAMYLLRDTTLPINDIVYRVGFNDLSYFNKIFRVQAQL
                     APSEYRGKYSVFNN"
BASE COUNT         1430 a          927 c          695 g         1385 t
ORIGIN      
        1 gcattccctg ttgccgcccc ctcaagatag ttacattata attgattcat aattttatct
       61 atttgttgat cttcgataat aattcaatct attaattcat ttattgattc ttgtttaaga
      121 tcttcaaaat ttttttacaa gcttaatatt atgtttgcaa ataaacttcg aacagttaat
      181 aaataattaa taccatattt caactttgca ataaacaaat ccgttatatt agtaacgcag
      241 ataatcatct taaaacagga taaccaatgt ttcaagaaat tacaataaag actattaaga
      301 ttcgtgttct taatattatg aattttgcaa aaattaattt tgcagaactt gcgaataatt
      361 acctgacttc gcctccccat gttttcaaga aagatcgtaa aattatagaa atttttactg
      421 atactgaact cgaaacaatc aacaacttta aggcattaaa aaaacagata gagtggatat
      481 caggtcgcgt ctgcataaaa accgttgcct ctgaaatttt gtataccgat cccacaaaaa
      541 taacaatatc ttatgaaaaa tatggagccc cctttattga tctatttccg gaaacttcat
      601 tttcaatatc ccattccgga gattttgctg gatgcgccat taacactcaa actcaatccc
      661 ctgtcggact tgacatcgaa caattaaaac cgattgatta tcctgggctt tcaaacataa
      721 ccgatgtatc ttttactgat aaagaaaaag gaaccataac agatagcgaa tcacttttta
      781 agatatggac aattaaagag gcatacctta aatactgccg tactggcttt cacgacacaa
      841 taaaaaatgt ggaatttttg aataactcca tcatgtataa aaatcaaatt gtgaatatta
      901 atcatcacac gcaatatata gacgattcac atattttttc actggtattt taatacatgg
      961 caaattacaa aacaacaagc agccctttgc acagtccgga gacacatctt gataaccgca
     1021 cgagacttct gtcaaatgga acccagggag agggagtcgt tatctactgg atggaacgtg
     1081 aactgagaat tagtgacaac catacccttg aagcagcaca acaccatgca cttttacata
     1141 acaaaaaact gcaaataatc tttattgttc acaattacgg aaccgagcgg caagcgcgcc
     1201 actaccgctt tgccttgcag gggttacttg aaacaataga tgaagccaac cgttatggtc
     1261 tgcctttttc ggttatcgtt ggcgatcccg ttaaagaaat tgtcaattta tgcaccaagt
     1321 acaaatgctg cgcactcttt acagatttta atccgctaag acatatccgc ctcaccaaga
     1381 aattgatctg tgaagagctt cccatgcccg tttttgaaac cgacgcccgt aacattattc
     1441 cctgtttcgc agccagccca aaacaggagt actcagcaca tacattaaga cttaaacata
     1501 acagacttat tgacgactac cacacgcccc tgccaaagat taaaaaatca gagctttcaa
     1561 aagccaaccc acatcctgat attgaaaaga tcataagaga tctgcatctg gactcaaaag
     1621 gtaattatga tcaccctttt attcccggtg cgaaagaggc gaaaaaatta ttgaaactct
     1681 ttttaacaaa gaagatccat gactactcca ccctggaccg agaccccaac cttgacgtta
     1741 tttcaaatct ttctccatat atacactttg gccagattgg tactcaccgt attttaagcg
     1801 aactgatcaa aacagaaata agcgatgata acagtgacaa gttttacgac cagctggtaa
     1861 taaggcgtga acttagcgag aattttgttt tctataatga taactacgac aattacaacg
     1921 gcattcccga ctggggcaaa cgcacactat tagcacacag agtggaccct cgcgattaca
     1981 actaccgttt tgatcaattc gaagccgcgg ttacccacga cgagctttgg aacgcagccc
     2041 aaaaacagat gatacaaacc ggcaagatgc actgctattt aagaatgtac tgggcaaaaa
     2101 aaattctgga gtggagtgcc tctcccgagg aagccttcaa tacggcaatc acccttaacg
     2161 accgctactc tctggacggc gaagacccca acggctacgc cggcgtcgcc tggagcattg
     2221 gcggcctgca cgaccgcccc tggtccgaac gccctgtttt cggctatgtg cgcttcatga
     2281 acctcgcagg ctgcaagcgc aagtttaatg tggataagtt tgttaaacgg tttatggaac
     2341 cgtaaaaagg tttttgtatt aacttagtaa atcccatccg catccctata taaaagaaca
     2401 gaatttttta tccacaacct ttttcataat caatccacca gcaagtcagt aatttcgaat
     2461 attaccgagt tttcgtaatc aacactaatc tcgatatcat caggagaatt gattacattt
     2521 tatttctata tgcttccgcg ttgtttccat tatttacagt tatcaattgc ccactatttt
     2581 caatgaaaac cttggacata aacgatacac catcgctcct gatcacttca gatttcattg
     2641 attcaaaatt atttgaagtt gaaacgataa gaactccacc gtcgttattt tcagatgaag
     2701 gcatcaacac tgcaactttt atattattat cagcatactg aattgatgcc atacaacttt
     2761 cataatcttc tataaacgaa cacttctcga cagaaattat cggcttagtt tcccaattct
     2821 caccattgtt gtttgaatac agaaaattcc ctttgttatc cactaggcat atttcattct
     2881 cgttattgct ctttatcgcc agaaattttg aagatgcagg ttttttaaca ggattccagc
     2941 ttacaccacc atctttggtg ttatagattt cacttttttt agttacgaca tacccctctt
     3001 gttcggatga gaagctaaca agagtgacat tatcgctaag cagtgttggt aaaattgacc
     3061 aggtctttcc tttatcgttt gaaattccaa cagttccgcc tcttccggca tgaacaaatt
     3121 tgccatctgg cattacatca atcgcgtatc ggcaaactgt ttcgttagct ccatttttcc
     3181 agctttttcc accatctatt gaatagtgag ttgctccgtc atatccggca ctaaaagcct
     3241 ctgtttttga aataaacgct acatttgtct gagcagaatc tatttcagaa ataacacatt
     3301 tctcccaatt tgatttttta ccacaaccaa gctgtaaagc aaatactagt agtgcaatac
     3361 ttgataaaac tatactgata attttattca ttttatccac ctcaatccat tttttcaaat
     3421 attgtacacg atatccaaaa aatatggaat agaggattat cagttaatcc tggcttattt
     3481 acagtaaaca accgtattta ttgatattct gtctaatttt aagttaacta aaactgactg
     3541 agactaaaaa taattaataa agtatcagtt attaaaaaca gagtatttgc ctctgtattc
     3601 tgagggagcg agttgcgctt gtacgcgaaa aattttatta aaatatgaga ggtcattgaa
     3661 tccaacccga tacactatat catttatagg aagagttgta tcacgaagca agtacattgc
     3721 aatattaatc ctgagcctgt tcaaatattc aattattgtt tgaccagatg ctttttgaaa
     3781 tcttttggat agtgttgttc tatcaataca gaacttttct gttatctcgt ttatggtaat
     3841 tttttcttta tagttggtat atagatacgc aataattttg tttatctcgt catcttttgt
     3901 gactgactta tccattgtca gaggatcaag tctgtttgaa taatttaata gttccaaaaa
     3961 atatgatctg gctctgcatg gccaataatc atctaccggg ttatcaagaa tttcatacat
     4021 tgatgatagc aaatccttaa ttcgcacttc actatttggg ccaagttgaa taaccgaccg
     4081 tttaccagcc ttattcaaaa aaagtcttaa gcagaataaa tccatacgtt ctgtacttgt
     4141 ataattgtta tctttagtat tcagtatatc tattgttaat ttactattta ttatatcagg
     4201 cttgaaaatc acattgcacg ctcttaattt atctgaatct gcacctgtga ttttctcctt
     4261 ttcggttaaa cataatagtg ccggagaaat aaagggaaga acccgtccat ttacgctgat
     4321 tgagccatta ccttcctgga aaaatattaa ccgataataa tcatcaatac catggtttaa
     4381 cagtaaatca ctgttttttt caataaaaag atacagctca taatcacata atacctt
//
LOCUS       DAOTGZ010000223         4414 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_3064
            NODE_23474_length_4414_cov_4.264740, whole genome shotgun sequence.
ACCESSION   DAOTGZ010000223 DAOTGZ010000000
VERSION     DAOTGZ010000223.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868088
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 4414)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4414)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:22:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,785
            CDSs (total)                      :: 2,751
            Genes (coding)                    :: 2,732
            CDSs (with protein)               :: 2,732
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 12 of 19
            Pseudo Genes (internal stop)      :: 5 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4414
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_23474_length_4414_cov_4.264740"
                     /isolate="AMR_MDS_3064"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            332..2920
                     /locus_tag="P5123_11770"
     CDS             332..2920
                     /locus_tag="P5123_11770"
                     /inference="COORDINATES: protein motif:HMM:NF014026.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 3 N-terminal
                     domain-containing protein"
                     /protein_id="HRX16982.1"
                     /translation="MYKDSSEPVEKRVSDLISKMSLEEKIAQLGGAMLLYIVDNNGFN
                     SKKAEYFLKNGIGQISRTQWMLGDIEPEECADLVTAVQKYLTEETRLGIPAIIHDECL
                     TGYQTKGSTAYPQSIGIAATWNEELAFKMTSYIRKEMRAVGATHGLSPVLDIVRDARW
                     GRVEETYGECPYLASAFATAFIKGLQSEDLKEGVLATGKHFIGYGVTEGGMNWSPSHI
                     NRRELLEVYARTFEASIQEANLGSIMNSYSEIDGVPCAASKEILTDLLRDTLGFKGVV
                     VAYYGAVMTAHTYHNLRKSAKEVAVETLKAGLDMELPKLEAYSFLGECVKDGTLDEEY
                     IDTACRRVLTKKFELGLFENPYGKKEDIPVLFGAEKCVALAKDICENSFVLLKNNGAL
                     PIASNTRKIAVVGPNAGSIRNLFGDYTYGGHLEGAFEMLHSFSTSDQSEPTDLQKAEK
                     LITDLYGLDIETCTFNELTEAYNNSDLAIKIKDITGVNPQTTTNPGQLWGAVDTIRHQ
                     RFMLPLERVRKLQKFSNIEELISYLYNPQSILEALKLKLSPDVQVQYAKGCEIIGDKD
                     LDIASAVKAAESSELIIAVMGDRSGIDQASTTGEARDRADLNLPGHQRKLLRELKKCG
                     KPLVLVLINGRPASLEWESENCDAILEVWIPGEHGAASIADTLLGNALPSGKLPISFP
                     RSVGQMPLFYNHKPSGGRSHWYGDYVEMSPKPLYPFGFGLSYTSFKYSSFSLSTDTID
                     KNGSVSVKLKIRNTGDYDGAETVQLYHTQQNVSVTRPVKQLIGYKKVFLKKSQSALIT
                     FTVPTEMFAFYNRENRYGVEEGTVKIMIGRSSEEIEYETDIYIESTFTVTDKIFFSTA
                     DFNIIE"
     gene            3085..3990
                     /locus_tag="P5123_11775"
     CDS             3085..3990
                     /locus_tag="P5123_11775"
                     /EC_number="2.3.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF010621.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase"
                     /protein_id="HRX16983.1"
                     /translation="MKGVFGFFLGISTFCLFITNTLFWCLPLTVFAFFKLIIPINAVR
                     ELITSIVNRIALNWTSVNNFFIKLLLPTRWIVNGLQENIEGWDRSKNYLVIANHCSAV
                     DIVALQMVFNNKIPFFRFFLKKELIWVPFLGLAWWALDYPFMKRYSREFLANNPEMRG
                     KDLDITRKACAKYQNSPVSIMNFPEGTRFTTVKHARQNSSYKYLLNPKAGGVGYVLSA
                     MGDNIHKILNVTLVYPGGIPGFASFFSGKIGKVVIDIEEIPVTEDIIGDYLNDDIFRK
                     DFQQKINRLWRDKDNKIEDLLVLHS"
     gene            complement(3970..>4414)
                     /locus_tag="P5123_11780"
     CDS             complement(3970..>4414)
                     /locus_tag="P5123_11780"
                     /inference="COORDINATES: protein motif:HMM:TIGR01784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="Rpn family recombination-promoting
                     nuclease/putative transposase"
                     /protein_id="HRX16984.1"
                     /translation="KDESDKILTDHLVLHFIELPKVKSNMGKNSLEIWSQYFINEGVD
                     NNIMKTLLKDEPMIVKAHDEFKKFTADERLRDIYESRLKAQRDHAYFMEIAREEGLEK
                     GMKEGIEKGIEKVAITLLDDFDDETIAQKTGLTRDHVRTLRVQNK"
BASE COUNT         1361 a          898 c          898 g         1257 t
ORIGIN      
        1 atgcgagcag caagagtcat cttgcgacca gcataggttg catatgcgag cagcccagca
       61 ccactttctt ttataaatgg tgctggacga tcagcatagg aactaacgga aaattccttt
      121 tgaataggca cttaaacgat gtgccttctg taatatgata atttttcgaa gttcccatat
      181 atcaaaaaca aaagtactct attaaagaat actaacatga agccctccgg ataaatgcaa
      241 tattcatgaa ggctattatt ttattctaaa atattatcac catactaaca gaactacaaa
      301 accaaaaact ttatcaagcg aggttttaac aatgtacaaa gacagctctg aaccagtaga
      361 aaaaagagtc agtgatctga tctctaaaat gagtcttgaa gaaaaaatcg ctcagcttgg
      421 cggggcaatg ttattgtata ttgtagataa taatggcttt aacagtaaaa aggctgaata
      481 ttttttaaaa aacggtattg gtcagatctc gcgaacacag tggatgttgg gcgatataga
      541 gccagaagag tgtgccgatc ttgtaacagc tgttcaaaaa tatcttaccg aagagacacg
      601 tcttggcata ccggctatta ttcatgacga atgtctcaca ggatatcaga caaagggaag
      661 caccgcctat ccgcaaagta tcggaattgc tgcgacctgg aacgaagagc tggcattcaa
      721 aatgaccagc tatattcgca aagagatgcg tgctgtaggg gcgacccatg gtttgtcacc
      781 tgttcttgac atagttcgtg acgcccgctg gggaagagtc gaagagactt atggagagtg
      841 cccctacctt gcatcagctt tcgctacagc ttttattaaa gggctgcaaa gcgaggatct
      901 gaaagagggc gtgctggcaa caggcaaaca ctttattggc tatggtgtca cagaaggcgg
      961 catgaactgg tcaccttcgc atatcaacag acgtgagctg ctggaagttt acgcacgaac
     1021 atttgaagcc tcgatacaag aggcaaatct gggatcaata atgaattcgt attcggaaat
     1081 tgacggagtt ccctgcgcgg catcaaaaga gattcttacc gatcttttac gggatactct
     1141 tggattcaaa ggagtagtag tcgcatatta tggagcagta atgactgcac atacctatca
     1201 taatctgaga aaatccgcaa aagaggtcgc tgtcgagact ttaaaggccg gacttgatat
     1261 ggaactgcct aagctagaag cctactcttt cctgggcgag tgcgttaaag acggaaccct
     1321 tgatgaagaa tatatcgata ctgcctgcag aagggtgtta acaaaaaaat tcgagcttgg
     1381 actttttgaa aatccctatg gtaaaaagga agatattcct gtactcttcg gcgcagaaaa
     1441 atgcgtcgcg cttgcgaaag atatttgtga aaattcattt gttctgctga aaaacaacgg
     1501 tgctttgcca attgcttcta acaccagaaa aattgcggtt gttggaccaa atgccggttc
     1561 gatccgcaat ctgttcggcg actacacata cggaggccac ctcgaaggcg cgtttgaaat
     1621 gcttcactca ttttcgacat ccgaccaatc tgaaccgaca gatttacaaa aggcagaaaa
     1681 acttataaca gatctttacg gacttgatat cgaaacctgc acttttaatg aacttaccga
     1741 agcctataac aacagtgatc ttgccataaa gatcaaagat attaccggtg ttaatcctca
     1801 gacaaccaca aacccagggc aattgtgggg agctgttgac actatacggc atcagcgttt
     1861 catgttaccc cttgaacgtg tgcgcaaact gcaaaaattc agcaatatcg aggagcttat
     1921 ctcttatcta tataaccctc aatcgatact ggaagctctt aaattaaaac tgtcaccgga
     1981 tgttcaggtc caatatgcga aaggatgtga aataatcgga gacaaagatc ttgatatagc
     2041 atctgcggta aaggcagctg aatcatcgga actgataatt gcagttatgg gagaccgctc
     2101 aggaatagat caagcctcca caaccggaga agcgcgtgac cgtgccgacc ttaaccttcc
     2161 cggacatcaa cggaaacttt taagagaact taaaaaatgc ggcaaacccc ttgtgctggt
     2221 tcttattaat gggcggccgg cttcgctcga atgggagtct gaaaactgtg atgcaatact
     2281 tgaagtgtgg attccgggtg agcatggagc ggcttcgatt gccgacactc ttttaggtaa
     2341 cgctttacct tcaggaaaac taccaatatc gtttccccgt tctgttggac agatgcctct
     2401 tttttacaac cataagccat caggagggcg ctcgcactgg tacggtgact atgtagagat
     2461 gagccctaaa ccgctttacc ctttcggttt tggcctaagc tacaccagtt ttaaatacag
     2521 cagcttttca ttatcaactg acactattga taaaaatgga tctgttagcg taaaacttaa
     2581 aatcaggaat accggtgact acgatggagc cgaaaccgta caactctatc acacacagca
     2641 gaatgtctct gtcacacgtc cggttaaaca gcttatcggt tataagaaag tttttctcaa
     2701 aaaaagccaa agcgccttaa taacatttac agttccaaca gagatgtttg ctttttataa
     2761 ccgtgaaaac agatacggtg tagaagaggg aactgtaaaa attatgatcg gtcggtcatc
     2821 agaagaaatt gaatatgaga cagacatata tatcgagtca acttttactg taactgacaa
     2881 gatctttttc tcaactgccg acttcaatat aattgaataa aaattgatct gagaaatatt
     2941 ataaaaaaag tttatagccc cggtgttacg aaacaacctg ggcaccataa actaacaagg
     3001 cataacacaa aacttgcctt gacaacattt catccggatg tgtttattac agcataaaaa
     3061 gtgattttta accggggttt gataatgaaa ggagtatttg gttttttttt gggtattagt
     3121 acattctgcc tttttataac aaacacactt ttctggtgtc tacctctgac cgtctttgcc
     3181 ttttttaaat taattattcc tatcaatgca gtcagagaac ttatcacatc gattgttaac
     3241 cgaattgccc taaactggac atctgttaat aattttttta taaaattatt acttcctacc
     3301 aggtggattg ttaatggttt acaggaaaac attgaagggt gggacagatc aaaaaattat
     3361 cttgttatag ctaaccactg ttcagcggtc gatatcgtcg ctcttcagat ggtatttaat
     3421 aataaaattc ctttcttcag gtttttcctg aagaaagagc ttatctgggt gcctttttta
     3481 ggacttgcct ggtgggctct tgattatcca tttatgaaac gctattcccg ggaatttctg
     3541 gctaacaatc cagagatgag aggcaaggac cttgatatta cgcgtaaagc ctgtgctaaa
     3601 tatcagaaca gcccggtctc aataatgaat tttcctgaag gaactcgctt taccactgtg
     3661 aaacatgcca ggcaaaattc gtcatataaa tatctgctaa acccaaaagc tggcggtgta
     3721 ggttatgttt tatccgccat gggcgataat atacataaga ttcttaacgt aacactggtc
     3781 tatccaggag gcatcccggg gtttgccagc tttttcagcg ggaaaatcgg caaagttgta
     3841 atagatatcg aagaaattcc ggtaacagaa gatataatag gtgactacct taacgacgat
     3901 atttttcgaa aagattttca gcaaaaaata aaccggcttt ggcgcgacaa ggacaataaa
     3961 atagaggatt tacttgtttt gcactcgtaa tgtacggaca tgatcccgtg ttaatccggt
     4021 tttttgcgct atagtttcat cgtcaaaatc atccagcaac gtaatggcta ctttctcgat
     4081 acctttctcg ataccttctt tcataccttt ctccagccct tcttctctag ctatttccat
     4141 aaaataggca tgatctcttt gagctttcaa tcgagactcg tatatatcgc gcaaacgttc
     4201 atctgctgta aactttttaa attcatcgtg ggcttttacg atcataggtt catcttttaa
     4261 tagtgtcttc ataatattat tgtccactcc ttcattaata aaatattgac tccatatttc
     4321 gagtgaattt tttcccatat tgctttttac ttttggtaac tcaataaaat gaagcacaag
     4381 atgatcggtt aatattttgt ctgactcatc tttt
//
LOCUS       DAOTGZ010000224         4380 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_3064
            NODE_23711_length_4380_cov_5.026358, whole genome shotgun sequence.
ACCESSION   DAOTGZ010000224 DAOTGZ010000000
VERSION     DAOTGZ010000224.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868088
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 4380)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4380)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:22:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,785
            CDSs (total)                      :: 2,751
            Genes (coding)                    :: 2,732
            CDSs (with protein)               :: 2,732
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 12 of 19
            Pseudo Genes (internal stop)      :: 5 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4380
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_23711_length_4380_cov_5.026358"
                     /isolate="AMR_MDS_3064"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1399
                     /locus_tag="P5123_11785"
     CDS             <1..1399
                     /locus_tag="P5123_11785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008622895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="helix-hairpin-helix domain-containing protein"
                     /protein_id="HRX16985.1"
                     /translation="KKACDIINRKYVKGAGNDSKIVSDAAIDGYGRLIKPSIENEFRK
                     AVKLKADNEAIKVFAYNVKELLLASPLGQKRLLALDPGYRTGCKVVVLDEKGELLFNT
                     AVYPHPPQQKRIETEDILKKLIKDYKIEAIAVGNGTAGRETEQLLREIAGDIPVFLVS
                     EQGASIYSASKTAREEFPDHDVTVRGAVSIGRRLVDPLAELVKIDPKSVGVGQYQHDV
                     DQKELKKSLEEVVEQCVNSVGVNVNTASRYLLEYVSGIGTTLATNIVEYRRKNGLFKN
                     REELKKVPKMGDKAYEQCAGFLRVSGGDTVLDNTGVHPESYGLVRKIAKDMKTSCEEL
                     IQNRALIESIPVQKYISEKTGEYTLKDIIQELKQPGRDPRPTIELFSFSADIMSIDDI
                     KEGMILPGKVTNVTKFGAFVDIGIKQNGLIHISNLSDSFVSDPGAVVKVNQCVNARVV
                     EVDYERGRIQLSLKN"
     gene            complement(1453..1890)
                     /locus_tag="P5123_11790"
     CDS             complement(1453..1890)
                     /locus_tag="P5123_11790"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRX16986.1"
                     /translation="MKSEEFLRILLFLLSVALLASIYLFFSRRSTLADLLELSEAHET
                     QRLALTDISRDLDAFREQNNLYETIEVTDNEQVETVDVPPSIDDIHNRLRATALEHAQ
                     AKWELAPRIKKLFRIVHLAGKWSLILGGIYLVFIFGYGFHSRY"
     gene            2023..2517
                     /locus_tag="P5123_11795"
     CDS             2023..2517
                     /locus_tag="P5123_11795"
                     /inference="COORDINATES: protein motif:HMM:NF015639.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YkgJ family cysteine cluster protein"
                     /protein_id="HRX16987.1"
                     /translation="MNNRYKALLSRAASQKRETKLFLEQLKRKKIKNLDKLFKKVTEE
                     IFYEIDCLDCGNCCRVLGPRINNSDMDRMSHFLGMRRAGFRNHYLTVDEDGDAVFKSM
                     PCPFLAQDDNTCAIYKARPRACMEYPHTDAKYMEKSLDLVFQNSLYCPAVILILDKIK
                     EEIK"
     gene            complement(2540..3463)
                     /locus_tag="P5123_11800"
     CDS             complement(2540..3463)
                     /locus_tag="P5123_11800"
                     /inference="COORDINATES: protein motif:HMM:NF012422.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LuxR C-terminal-related transcriptional
                     regulator"
                     /protein_id="HRX16988.1"
                     /translation="MFRYLLFFNIATIIMGALAIYTAGILAIKYRQKILKTYSLFLIF
                     YLLSILNSTFYVMEFLFFFRAIDTPNIYLLFFSIQVVIIFSVSSSQFFLTNSFFNSEF
                     WTFKKQIVFVIAIITISAICGLYVFIKRYNWITVNFMTIVSTVILIAASFHFIAIALL
                     NIKKEQLFRRKIIIFTIFLCILTCFLTCIDYIPYFLGASTRHSFIIKGFMFSNLHIFL
                     LYCFSVYAGLPYILKEPISHREMNFQQILNRYSITSREQEILEKIVQGYSNIQISAEL
                     NISLSTVKSHIYNMFQKTSSENRIELINVFK"
     gene            complement(3466..4281)
                     /locus_tag="P5123_11805"
     CDS             complement(3466..4281)
                     /locus_tag="P5123_11805"
                     /inference="COORDINATES: protein motif:HMM:NF015605.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HRX16989.1"
                     /translation="MGATDISVLSMFLFSSIILLPLIAGIFLKLGITKNLIYSLTRMS
                     IQLLLVGVYLKYLFEYNNIFVNTAYIFIMITVAAFSVIRSSSLSLSKTVFPVFWSIVI
                     PQIVVLALFNWTTVGITELFNAKYLVPIGGMILGNCLSGNIITLSSFYKSINKDEKKF
                     LQMISYGADRSEAIAPYLRASMLASINPALSRMATMGIVSLPGMMTGQILGGSVPMVA
                     IKYQILIMIAIFSASFFSGLLSIYFTLPVSFTACGTLNKKIFNLQSNSPTKGK"
BASE COUNT         1453 a          683 c          943 g         1301 t
ORIGIN      
        1 aaaaaaagct tgtgatatta taaacaggaa atatgtgaaa ggggctggta atgattcaaa
       61 aatagttagc gatgccgcta ttgacggata tggccggctt attaaaccat ccatagaaaa
      121 cgagtttaga aaggctgtaa aactaaaagc agataatgaa gctatcaaag tatttgcata
      181 taacgttaag gagttgcttc ttgcttcgcc attaggtcaa aaaagactat tggctctgga
      241 tcccggatat cgtacgggtt gcaaggttgt tgtgttagat gaaaagggtg agctgctgtt
      301 taatacagcg gtttatcctc atccgccaca gcaaaagcga attgaaaccg aagatatatt
      361 aaaaaagctg attaaagatt ataaaataga agcaattgca gtaggaaacg gaacagccgg
      421 tcgggagacg gaacagctgt tacgtgaaat tgctggagat attccggtct ttcttgtgag
      481 tgaacagggt gcttccattt attcggcatc aaaaactgca agagaggagt ttcctgatca
      541 cgatgtaacc gttcgcggcg ctgtctcgat aggtcgaagg ctggtcgatc cgttggccga
      601 gcttgtgaag attgacccta agtctgtagg tgtgggccaa tatcaacatg acgtagatca
      661 gaaagagctt aaaaaaagtt tggaagaagt tgttgaacag tgcgtgaact cggtaggtgt
      721 taatgttaat acggctagcc gttatcttct tgagtatgtt tcgggtatag gaactacttt
      781 ggctactaac atcgttgaat atcggcgaaa aaacggacta tttaaaaacc gtgaagagct
      841 gaaaaaagtt cccaaaatgg gagataaggc ttatgaacag tgcgcgggtt ttttgcgtgt
      901 ttcgggggga gatactgtgc ttgacaatac aggggttcat cctgaatcgt acgggctggt
      961 tcgaaaaatc gcgaaagata tgaaaacaag ctgtgaagag ttgattcaaa acagggcttt
     1021 aatcgagagt atacctgtac aaaaatatat ttctgaaaaa accggtgaat ataccttaaa
     1081 ggatatcata caggagctta agcaaccggg gcgagatccc cgtccgacaa tcgagctttt
     1141 tagttttagc gctgatataa tgtcaataga cgatatcaaa gagggaatga ttttgcctgg
     1201 aaaagtaaca aatgttacga agtttggagc ttttgtggat ataggaatta aacaaaacgg
     1261 tctaattcat atatccaatt tatctgactc ttttgtgagt gatccgggag ctgttgtaaa
     1321 ggtcaaccaa tgtgtaaacg cgagagtagt tgaagttgat tatgagcggg gaagaattca
     1381 actctcgctt aaaaactgac ctgttatatt gcggatggtt ctttggtatt ttgacttgtt
     1441 tttataataa aatcaatagc gagagtgaaa cccataaccg aaaataaaaa caaggtagat
     1501 gccccctaat attagagacc attttccagc aaggtgaaca attctaaata gtttttttat
     1561 ccgtggagca agctcccatt ttgcctgagc gtgttcaagg gctgttgccc gcaagcgatt
     1621 atgtatgtcg tcaattgaag gtggaacatc aactgtttcg acttgttcat tatccgtcac
     1681 ttctatagtt tcgtaaagat tgttttgttc ccgaaaggca tccaggtcgc gcgaaatatc
     1741 agtaagggca agtctttgtg tctcgtgtgc ttcagagagt tccagtaaat cagccaaggt
     1801 acttcgtctc gagaagaaaa gatatattga cgcaagtaat gctacagata gcaaaaagag
     1861 cagtatacgc aaaaattctt cggatttcat ataatcgcct ctgtaatttt tataacacta
     1921 tcagattgta ttatttactg attgtgtaaa gtaataaatt attcgcgttt atatggtttg
     1981 tttttttgct tattggtttt aaaagtaaaa aaggggattc ttatgaacaa tcgttataaa
     2041 gctttattgt ccagggcagc ttctcaaaaa agagagacaa agttgttttt agaacagctt
     2101 aaacgtaaaa agataaaaaa tttggataag ctttttaaaa aagttacgga agagattttt
     2161 tatgaaattg attgcctgga ttgtggaaac tgctgcaggg ttttagggcc aaggataaat
     2221 aactctgata tggatcgaat gtcccatttt ttgggaatgc gtcgtgctgg ttttagaaat
     2281 cattatctta ctgttgatga agacggtgat gccgttttta aaagtatgcc atgtcctttt
     2341 ttggcacaag acgataatac ctgtgcgata tacaaggcga ggccgcgtgc atgtatggaa
     2401 tatccgcata ctgacgcgaa atatatggaa aaatcactgg atcttgtttt tcaaaacagt
     2461 ctctattgcc ctgctgttat actgatactt gataaaataa aagaagagat caaataacat
     2521 cttgttttat aaaacacact tatttgaaaa cattgatcag ctcaatacgg ttttctgaag
     2581 atgttttttg aaacatattg tatatatgac tttttacagt agataaagag atattaagct
     2641 cggcgctgat ctgaatatta ctgtatccct gtacaatctt ttcaagaatt tcctgttcgc
     2701 gtgatgttat tgaatagcgg ttaagtatct gttgaaaatt catctcgcgg tgtgatattg
     2761 gctctttcaa aatatagggg agcccggcat atactgagaa gcagtatagt aaaaaaatat
     2821 gtagattcga aaacataaag cccttaataa taaaagagtg tcgtgttgag gcaccaagaa
     2881 aatagggaat ataatcaata catgttaaaa aacaggtaag aatacaaaga aaaattgtaa
     2941 aaatgataat ttttcgtcta aacagctgtt cttttttaat atttaataat gctattgcta
     3001 taaaatgaaa cgaggcggca ataagtataa cggtagaaac aattgtcatg aaatttacgg
     3061 ttatccagtt ataacgtttt ataaatacgt ataatccgca tattgcagat atagtgataa
     3121 ttgctattac aaaaactatc tgttttttaa aagtccagaa ctcggagttg aaaaaactgt
     3181 tggttagaaa aaactgagac gatgaaaccg aaaaaataat taccacttga atgctgaaaa
     3241 aaagtaaata gatatttggc gtatcaattg ctctgaaaaa aaacaaaaac tccataacat
     3301 aaaaagtact gtttaaaatt gataaaaggt aaaagatcag aaaaagagag tatgttttta
     3361 aaattttctg tctatatttt atagcgagga taccggcagt ataaatcgcc aaggcgccca
     3421 ttattatagt agcaatattg aaaaacagta aatatctgaa catatctatt ttccttttgt
     3481 gggtgaattg ctttgtaaat tgaatatctt tttgtttaaa gttccgcagg cggtaaatga
     3541 taccggaagt gtaaagtata ttgatagtag ccccgaaaaa aaggaggcac tgaatattgc
     3601 tatcatgatt agtatctgat attttatcgc gaccataggt accgagccac caagaatctg
     3661 tcctgtcatc attccgggta gtgagacaat acccatggtg gccattcggc taagtgccgg
     3721 gttaatgctg gcgagcatgc ttgcgcgaag atagggagct atggcttcgc ttctgtcggc
     3781 tccgtaagag atcatctgta aaaatttttt ttcatctttg tttatggatt tatagaatga
     3841 agatagggtg ataatgttgc cggaaaggca gttgcccaaa atcattccac ctattggaac
     3901 aagatatttt gcgttaaata gttcggttat tccgactgtc gtccagttaa atagcgcaag
     3961 tacaactatt tgtggtataa cgattgacca gaaaacgggg aaaaccgttt ttgacaaaga
     4021 taacgaactg gatcgtataa cggaaaaagc cgcaacagta atcattatga aaatatacgc
     4081 tgtattgaca aaaatattgt tgtactcaaa gagatatttg aggtacacgc ctaccaataa
     4141 aagttgtata ctcattcgtg taagtgaata aatcagattc tttgtgatac caagcttaag
     4201 aaatatcccc gcgataagcg gaagaagtat gatcgatgaa aatagaaaca ttgacagtac
     4261 ggatatgtcg gtagcaccca tattaaatct ccagaatatt tatttcggac gaattgtgcc
     4321 attcggggtc atgcgagact attaacgcgg ttttatcgct ttccataatt tttgagacta
//
LOCUS       DAOTGZ010000225         4372 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_3064
            NODE_23770_length_4372_cov_4.176975, whole genome shotgun sequence.
ACCESSION   DAOTGZ010000225 DAOTGZ010000000
VERSION     DAOTGZ010000225.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868088
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 4372)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4372)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:22:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,785
            CDSs (total)                      :: 2,751
            Genes (coding)                    :: 2,732
            CDSs (with protein)               :: 2,732
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 12 of 19
            Pseudo Genes (internal stop)      :: 5 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4372
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_23770_length_4372_cov_4.176975"
                     /isolate="AMR_MDS_3064"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..308)
                     /locus_tag="P5123_11810"
     CDS             complement(<1..308)
                     /locus_tag="P5123_11810"
                     /inference="COORDINATES: protein motif:HMM:NF013731.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis protein CheW"
                     /protein_id="HRX16990.1"
                     /translation="MLRDMQKKETTSLQVVCFKIGEEEYGLEILKVQEILKLPKVTKL
                     PKSASFIIGMIDLRGQVIPIINLSNKFGIKEVSDHKNLRAIVVDIQGKKVGLAIDSVS
                     "
     gene            complement(484..1218)
                     /locus_tag="P5123_11815"
     CDS             complement(484..1218)
                     /locus_tag="P5123_11815"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRX16991.1"
                     /translation="MRGSSNRARANQLVGDNTASSGVYWKRRPSEIKGYQRAFYYNDP
                     DGNYIRVDYNTKSQKVRIFYESADEGGIAYYSVIEKGDVTSERNMTSGRTTSLTAKFS
                     ALAPLFASLPNKEVLRLMGNNYGLADYSKELKEKLKRQKLEKTRQRYFKQEKIDRDPF
                     LGDFDPGKGIGFKDFMDMGAGAVVSLAIYYYIFNYQVLGGFLAIYGISLGLIDIFLRE
                     REPIFFKIIIFLITGVVLFIYGYYIN"
     gene            complement(1477..2415)
                     /locus_tag="P5123_11820"
     CDS             complement(1477..2415)
                     /locus_tag="P5123_11820"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRX16992.1"
                     /translation="MKKFFAFVFIITIIASAGYFAYSSVKFAPKGQILVAYDNSEKRI
                     IALSGNGWIVAPYAVIPGRVQFYSIEPDGFFEVECSLSLGELDILDDPAYNVSVTLVL
                     NYTVDSKVYVPSSSQLKDPSGAIVGRLQSELKSEFADVINPYLEPPFDSEKLTLNWDD
                     ILLAIEKKIRERAIKYGIDVTSLKAQTILQVPSREMYEYGISLRNDLMELRKKHLIER
                     ENLNNALNLKSVETGKYYEHLQKISDIIATNPDILKYIYIEKLAPNVKIIAPINSNGY
                     AFGLDNIDESEKDVKKLEKSDKPESKDKDGEVDNLR"
     gene            complement(2422..2640)
                     /gene="hfq"
                     /locus_tag="P5123_11825"
     CDS             complement(2422..2640)
                     /gene="hfq"
                     /locus_tag="P5123_11825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002740964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_process: GO:0006378 - mRNA polyadenylation [Evidence
                     IEA];
                     GO_process: GO:0016070 - RNA metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA chaperone Hfq"
                     /protein_id="HRX16993.1"
                     /translation="MSVNIQDKILNNARKEKVDITIYLTNGVPLQGKVQAFDNFTVLL
                     RVDTKQNLIYKHAISTIMPSKPLKYEDE"
     gene            complement(2717..3631)
                     /gene="miaA"
                     /locus_tag="P5123_11830"
     CDS             complement(2717..3631)
                     /gene="miaA"
                     /locus_tag="P5123_11830"
                     /EC_number="2.5.1.75"
                     /inference="COORDINATES: protein motif:HMM:TIGR00174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0052381 - tRNA dimethylallyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (adenosine(37)-N6)-dimethylallyltransferase
                     MiaA"
                     /protein_id="HRX16994.1"
                     /translation="MIPGPTASGKSDAALCICDLFTDAFEIVSVDSVQVYRELDIGSG
                     KINASQRKSVPHHLIDVVNPDEHFDFVTYCALADKAVDDIQKRKKIPLFTGGTLLYLD
                     TYFFGYSPMPDSDPLVRDMLLKKMKDLGINALHHELANTDPVSAAAIHPNDSQRILRA
                     LEVHYITGKKMSDLRGQKKGRIGEETIFISPKIDRETLYNRINERVNQMISSGFIDEV
                     QKLIDKGYDKSLNSMNSIGYSQIYDYLKSKCDLNFAIEDIKKKTRKYAKKQITWLKRY
                     DFIKYFDMTDRKGLSSFVNECFLLTDNH"
     gene            complement(3639..3851)
                     /locus_tag="P5123_11835"
     CDS             complement(3639..3851)
                     /locus_tag="P5123_11835"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRX16995.1"
                     /translation="MIIPLDKLNALESNKFVFTKGAMMTVMRLNKIKNYPEPDLNWKI
                     VPNVLKLVLNEEIHYQKNEMRVEKKD"
     gene            complement(3920..>4372)
                     /locus_tag="P5123_11840"
     CDS             complement(3920..>4372)
                     /locus_tag="P5123_11840"
                     /inference="COORDINATES: protein motif:HMM:NF012832.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="guanylate kinase"
                     /protein_id="HRX16996.1"
                     /translation="VDKDTFRKEIENEGFLEWAIVHNNYYGTRKKEIDRISGEGKIPV
                     LDIDVQGASILRKKIQDAVFVFIVPPSLEVLEDRLRNRKTDSDEQIGIRLKASILEMR
                     SCAEFDYAVVNDDVTTAVSDIVSIIRSEKLRIERMKQKISDICDNKNL"
BASE COUNT         1342 a          901 c          649 g         1480 t
ORIGIN      
        1 tggcttacag agtctatagc caatccgact tttttcccct gtatatcaac aactattgct
       61 cgcagatttt tgtgatcact aacttctttt ataccaaatt tgttggaaag attaataatg
      121 ggtattacct gtccacgcag atcgatcata ccaattataa acgaagcaga ttttggtaac
      181 tttgtaacct taggaagttt taatatctct tgaactttta gaatttccaa cccatattct
      241 tcttcaccga tcttgaaaca tacaacctgc aaagaagttg tctctttctt ttgcatatct
      301 ctaagcataa acaccacctg tattacagta aggagacatc gattatttac aatgccccag
      361 gaaagttgtt cttaattatc agcaattaat aaaaaagccg ttaacagatt ctcatagtaa
      421 tcataccaaa gcaataattc aagcataaat taattgaata tgtatttagc agagcaaaag
      481 cagctaattt atatagtaac catatataaa caatacaact cctgtaatta gaaaaataat
      541 tattttgaaa aatattggtt ctcgctctct caagaaaata tcaattaatc caagcgatat
      601 accataaatt gctaagaacc ctcccaaaac ctggtaattg aagatatagt aataaattgc
      661 gagcgatact acagcacctg cccccatatc cataaaatcc ttgaagccta tccccttacc
      721 cggatcaaaa tcacccaaaa atggatccct atcaatcttt tcctgtttaa aatacctctg
      781 gcgggttttt tccagttttt gccgttttaa cttctctttt agttcttttg aataatcagc
      841 taaaccataa ttattgccca ttaaacgaag aacttcttta ttcggcagtg aagcgaaaag
      901 tggcgccaga gcggaaaatt tagctgtcaa ggaagttgtt cttccagaag tcatattgcg
      961 ttcagatgtg acatcacctt tctcaataac agaataatat gcgataccac cctcatcggc
     1021 ggactcgtaa aagatacgca ccttttgaga ttttgtatta taatcaactc ggatatagtt
     1081 tccatcaggg tcattatagt aaaaagctct ctgatacccc ttaatttcgg aaggtcttct
     1141 tttccaatag acaccagatg aggcggtatt gtcaccgaca agctgatttg ctctcgctct
     1201 gttgcttgat cctctcatag tgctcccctt atatccttgt ttcgtaaatt caacaaaaaa
     1261 gctgccctaa ttggacagct tctctaaaga tattcctgtt tgaaatcaaa caaagaaccg
     1321 agtacaaaca ggttatatat tactttttct tatgacgatt ttgtcttctg cgcttttttc
     1381 ttttatgatt agctatcttt tgacgctttt tcttttttcc gcacggcatc gcaaactcct
     1441 tattattgat taaaaccctt ttgcaaagaa aatcagttag cgaaggttat cgacctcacc
     1501 atctttgtct ttactttcag gtttatcact tttttccaat ttttttacat ctttttcaga
     1561 ttcatctata ttgtcaagac caaaagcata cccattagaa ttaatagggg caataatctt
     1621 aacatttggt gccaattttt ctatatagat atactttaaa atatctggat ttgttgcaat
     1681 aatgtccgat attttttgta aatgctcgta atatttccct gtctctacac tttttaaatt
     1741 gagtgcatta ttgaggtttt cacgttctat caaatgtttt tttcgaagtt ccatgagatc
     1801 atttcgtaaa gaaattccat attcatacat ctctctgctt ggtacttgaa gaatagtttg
     1861 agcttttaac gatgtaacgt ctattccata ctttatagcc ctttctctta tctttttttc
     1921 tatagctaaa agtatatcat cccaatttaa agtaagcttt tcagaatcga aagggggctc
     1981 taagtacggg ttgatgacat ccgcaaattc agattttaat tcggattgca acctgccaac
     2041 aatagcaccc gacggatcct tgagctgaga actggaggga acataaacct ttgagtctac
     2101 agtataattc agcacaagag ttaccgaaac attatatgcc gggtcatcca gtatatcaag
     2161 ctctccaaga gagagagaac actcaacttc aaaaaaacca tcaggttcta tggaataaaa
     2221 ttgaaccctg cctggaatta ccgcataagg agcaactatc cacccgttgc ccgaaagagc
     2281 aataatcctt ttttcactat tatcatatgc caccaaaatt tgccctttgg gagcaaactt
     2341 gacagagcta tatgcaaaat agcctgccga ggcaataatc gttataataa aaacaaaggc
     2401 gaaaaacttt ttcacaataa atcactcatc ttcgtatttt aacggcttag aaggcattat
     2461 agtagaaata gcatgtttgt aaataagatt ttgcttggta tcaactctta aaagaacagt
     2521 aaaattatca aaagcctgca ccttaccttg aagaggcaca ccattagtaa gataaattgt
     2581 aatatccact ttttcttttc tggcattatt taagattttg tcctgaatat taacactcat
     2641 attaactccc ccttttttta tctgtatatt tgtagcccga ccttttaaag aaacaaagcc
     2701 acaggaagca gagcctctaa tggttatctg ttaacaaaaa acattcattt acaaaactac
     2761 tcagaccctt gcgatctgtc atatcaaaat atttgataaa atcatatcgt ttcaaccaag
     2821 ttatctgttt ttttgcatac tttcttgtct ttttctttat atcctctata gcaaaattca
     2881 gatcgcattt acttttaaga taatcatata tctgagaata tccaattgaa ttcatcgaat
     2941 tgaggctttt atcgtaacct ttatctataa gtttctgaac ctcatcaata aaccccgatg
     3001 atatcatctg atttacacgc tcgtttattc ggttatatag tgtttcccga tcaatttttg
     3061 gcgatataaa tatcgtttct tccccaattc ttcctttttt ttgtccacga agatcactca
     3121 tttttttgcc ggtgatataa tgtacttcga gcgcccgcaa tattcgctgt gaatcgttag
     3181 gatgtatggc tgctgctgat actggatcag tattggccaa ttcatgatgt aaagcgttga
     3241 tgcctaaatc tttcattttt tttaacaaca tatctctaac caatggatca gaatcgggca
     3301 ttggtgaata tccaaaaaaa taggtatcaa gatataacaa agtacctcct gtaaaaagag
     3361 gtatcttttt tcgtttttga atatcgtcaa ccgctttatc ggccaaagca caatacgtta
     3421 caaaatcaaa atgttcatca gggttaacga catcaataag gtgatgaggt accgacttac
     3481 gctgcgaagc atttatcttg ccgctaccaa tatccagctc ccgataaact tgaactgaat
     3541 caacagacac gatctcaaaa gcatcagtga acagatcaca tatacataag gctgcatcac
     3601 ttttacctga ggccgtaggc ccaggaatca caagggctct aatctttttt ttccactctc
     3661 atttcatttt tttgataatg tatctcttcg ttaagtacca atttaagcac gtttggaact
     3721 atcttccagt taagatccgg ttccggatag tttttaattt tgttaaggcg cataaccgtc
     3781 atcatggccc ctttagtaaa aacaaacttg tttgattcaa gcgcgtttaa cttatccaaa
     3841 ggtataatca ttttttcatc ccgtttatct ataaaaattg tttatgtcgg cataaacatg
     3901 ttttgatcac tataatactt caaaggtttt tattatcaca aatatcgctg attttttgtt
     3961 tcattctttc tatacgtaat ttttctgatc tgattattga tactatatcc gaaacagcgg
     4021 ttgtaacatc atcatttacc accgcgtaat cgaattcggc gcatgatcgc atctcaagaa
     4081 tcgaagcctt tagccgtata ccaatctgtt cgtcagagtc tgttttgcga tttcgtagtc
     4141 gatcttcgag aacttcaaga gaaggaggca ctataaatac aaaaacagca tcctgtattt
     4201 ttttccttaa aattgaggca ccctgcacgt ctatatcaag tacaggaatt tttccttcac
     4261 ccgatatcct gtcaatctct tttttacgtg taccataata attattgtgt acaattgccc
     4321 attcaagaaa accttcattt tcaatctctt tcctgaatgt gtccttatcc ac
//
LOCUS       DAOTGZ010000226         4348 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_3064
            NODE_23946_length_4348_cov_5.270207, whole genome shotgun sequence.
ACCESSION   DAOTGZ010000226 DAOTGZ010000000
VERSION     DAOTGZ010000226.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868088
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 4348)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4348)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:22:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,785
            CDSs (total)                      :: 2,751
            Genes (coding)                    :: 2,732
            CDSs (with protein)               :: 2,732
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 12 of 19
            Pseudo Genes (internal stop)      :: 5 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4348
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_23946_length_4348_cov_5.270207"
                     /isolate="AMR_MDS_3064"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..210
                     /locus_tag="P5123_11845"
     CDS             <1..210
                     /locus_tag="P5123_11845"
                     /inference="COORDINATES: protein motif:HMM:NF014299.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRX16997.1"
                     /translation="IDPDIAYNRVASREKNNNLECFETPTKLRLIDSIYREIIPENTV
                     TIDAALSPDETAKQVYMHIKDYIVP"
     gene            207..1565
                     /locus_tag="P5123_11850"
     CDS             207..1565
                     /locus_tag="P5123_11850"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRX16998.1"
                     /translation="MIKKAYTVLVNVSFTALLTAIAYKAKDLDVRVEYFILFIIIFTV
                     VLFPIIDYFINNTPFLKRKKVRTELYLDTIDSIIQLDSIDELLSLQFNKLLSVISLSE
                     GRMIFYDREKDEYTLYLKTLKPDSFTAIEKLEYNNILLEVLNTPQDIIQKNRIGDALQ
                     FERRLLSEMTRLNTEIIIPMFFSDILTGALLLGKRESPYSDDDLSALKIFASKVASLS
                     MNAAFWKEMTRKIELEKERKTELRVQRSFLPMANRIMPHAQAVIYFLMSSILFDRFYN
                     LFRYKNSIYFISYTIIRDNRSTLIFLPSLAVLFETFIKSDMNIEQSIYEARQILADKG
                     MYESSPRLFVSSLNSDGNFFFINETKVTPWLFRDGFTKLTESTRLQENDICLYMNRYT
                     GNLILTHSEKVKKIIVSNRNNTADKIKTALVDFLSEENRIRENLFLCLFKFTGARNDQ
                     DQ"
     gene            1549..3927
                     /locus_tag="P5123_11855"
     CDS             1549..3927
                     /locus_tag="P5123_11855"
                     /EC_number="3.1.3.16"
                     /inference="COORDINATES: protein motif:HMM:NF018884.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004722 - protein serine/threonine
                     phosphatase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PP2C family protein-serine/threonine
                     phosphatase"
                     /protein_id="HRX16999.1"
                     /translation="MIKISNYKTNAWIRKVAALAILLFLDIYFITSLYDFAYKYPFSR
                     VIIDSTLHHSVDMFNKDHLFKATIVSINDKPVTGSPLKVILDTKVDIRKAVIKKKDLN
                     HTIQFRQNRFNSDFFIFSFFILIIANLHFIWGLILYFAKRLYSSSRYYSAASTALGLF
                     FFSTIHYFISGNSAPLFACAFLFSFIIIRILLHLSRLRFSKLMQVLVALVFIITAFAI
                     ITVVSPLKVIHYFKIYLYLFLGSSLIFGITAIIKNIGNRYRNKALLFLLAASTCGLYI
                     PFLLIIPMLNFDFPIPISITSLLTIIAPIVIGKTLIENSQISELITRNFNSRLIIIDI
                     AASILISILLAGVLFMSQVTRFLYLPMLFALLNIALACRKFIQSGKHHHEVSGKDLQT
                     SSLQRIIETSVMPIDFSQRITRIYREISLYLDVESVKLAVFSSEQLNNTLKSSELVMT
                     LDSDNPLNEHYRNNNDIIKRDMLYNSKIDSFMSKTPDMDKYYLIVPVFFRNSITALFF
                     LTQKVRRTPYFKSEYEYISTAGSIAFQTLENESLIHQSIIKSRYEQDLDNASYVQMRL
                     FPVYVPSETGLELSLYTRPYNKVTGDYLDVVEIDEFRTAIMIGDITGHGLPAAMLHST
                     TSVLITALFKQGKTLTEIFYAINDFLINKYLGHELITMFGAIFDKRTSSMEYVNAGHP
                     PVYVIHPEKETFMRVESKGHILGVTDTAVYTPQKLTLSQKSQLFFFTDGLTEIKKEKV
                     DSNIGEEHLEKMLKNMLKESVEKKMEGLTYFISKVKADCISDDITIAGVEIS"
     gene            complement(3986..4156)
                     /locus_tag="P5123_11860"
     CDS             complement(3986..4156)
                     /locus_tag="P5123_11860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010075348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HRX17000.1"
                     /translation="MAYKITDSCTSCGSCESECPVEAISQGDSQYEIDESACTSCGLC
                     ASVCPVDAIIEG"
BASE COUNT         1397 a          920 c          689 g         1342 t
ORIGIN      
        1 atcgatccag atatagcata taatagagtt gcttcgcggg aaaagaacaa taatctggaa
       61 tgtttcgaaa cacctactaa gctacgatta attgattcaa tttacagaga aattattcct
      121 gaaaataccg ttactattga tgcagctctt tctcctgatg aaaccgcaaa acaggtttac
      181 atgcatatta aggattatat tgttccgtga taaaaaaagc atatacagtt cttgtaaacg
      241 taagctttac agccctactg acagctattg catataaggc taaagatctg gacgtacggg
      301 tcgagtattt catacttttc ataataattt ttactgttgt tctgtttccg attattgact
      361 attttataaa caacactcca tttttaaaac gaaaaaaagt acgtacagag ctctaccttg
      421 acacgattga ttctatcatt cagcttgact caattgatga gctgctaagc cttcagttta
      481 acaaactgct atccgttatt tcgctttcag aaggccggat gatattttat gacagggaaa
      541 aagacgaata caccctatat ctaaaaactc ttaaaccgga ttctttcact gctatcgaaa
      601 aacttgagta caacaacata ctacttgagg tactcaatac gccacaagat atcattcaaa
      661 aaaaccgaat cggtgacgct cttcaatttg aaagaagact gctttcagaa atgacacggc
      721 ttaacaccga aatcataatt ccaatgtttt tttcagatat ccttacaggc gcgcttcttc
      781 taggcaagag agaatcacca tactctgatg atgatctttc cgcgcttaag attttcgcct
      841 ctaaagttgc ctcattgtcc atgaacgccg ctttttggaa agaaatgact cgcaaaatag
      901 aattggaaaa agagagaaag accgagctta gggtacaacg ttcatttctt ccaatggcca
      961 accggatcat gcctcacgca caggctgtaa tctattttct tatgagctca attctttttg
     1021 atcggtttta taatctgttc agatataaaa attccatcta ttttatttcg tacacaatca
     1081 tacgtgacaa cagatccaca ttgatttttc tgccttcact tgctgtcctg tttgaaacat
     1141 ttataaaatc cgacatgaac atagagcagt ccatctacga ggccagacaa atacttgccg
     1201 acaaaggaat gtatgaaagt tcaccgcgcc tttttgtaag ctcgcttaac agtgacggta
     1261 atttcttttt tataaacgag acaaaagtta caccttggct tttcagagac ggattcacaa
     1321 aacttaccga atcgacacgt ttgcaagaaa acgatatttg cctctatatg aaccgctaca
     1381 ccggcaacct tattctcact cattccgaga aagtaaaaaa aataattgta tcgaatcgca
     1441 ataatacagc tgataaaata aaaacagcat tggttgattt tttatctgaa gaaaaccgta
     1501 tccgagaaaa tctttttcta tgtcttttta aatttacagg agcgcgtaat gatcaagatc
     1561 agtaattata aaacaaacgc gtggatccgc aaagtagctg ctctggcgat acttctcttt
     1621 cttgatatat attttattac atccttgtac gactttgctt ataaatatcc attttcacga
     1681 gtaatcatag attcgaccct tcaccactct gtcgacatgt ttaacaagga tcaccttttt
     1741 aaagctacca tagtttcgat caacgataaa cctgttactg gctcaccgct aaaagtaata
     1801 ctcgatacta aggtagacat ccgaaaagct gtgataaaga aaaaagacct aaaccataca
     1861 atccagtttc ggcaaaatcg gtttaacagt gattttttta ttttcagttt ttttatactg
     1921 ataattgcca acctgcactt catctggggg cttattttat actttgccaa aaggctctat
     1981 tcgtcatcgc gatactactc ggctgcatca acagcactcg gtctcttttt tttcagtaca
     2041 attcactatt ttatttcggg aaacagcgct ccgcttttcg cctgtgcttt tttattttct
     2101 tttataatta tcagaatttt attacatctt tcgcggctca gattttcgaa attaatgcag
     2161 gtactagtcg cacttgtttt tataataacg gcttttgcaa taattaccgt cgtctctcca
     2221 ttaaaagtaa ttcattattt taaaatttat ctttatctgt ttttgggaag ctcacttata
     2281 ttcgggataa ccgcgataat aaagaacata ggtaaccgtt accgcaacaa ggctcttctt
     2341 tttcttttgg ccgcttcaac ttgcggctta tatattcctt tcctactgat tattcccatg
     2401 ctgaattttg attttcctat accaatcagc ataacaagcc ttttaacaat aattgcccct
     2461 atcgtcatcg gcaaaacact tatcgaaaac agccagataa gcgaactaat aaccagaaac
     2521 ttcaactctc gtctgataat aattgatata gcagcctcga tactaatttc cattttgctt
     2581 gctggtgttc tttttatgag ccaggttacc aggtttttat atttacccat gctcttcgcc
     2641 ctactcaata ttgctcttgc ctgccgaaaa tttatacagt caggcaagca tcatcacgaa
     2701 gtatcaggca aagatctaca gacttcctcc ttgcagcgaa tcatcgaaac ttctgtaatg
     2761 cctatagatt tttcgcaaag aataactaga atttaccgcg aaatttcact ctatcttgat
     2821 gtcgaatccg taaaactggc tgttttcagt tcagaacaac ttaacaatac ccttaagtca
     2881 tccgaacttg tcatgactct agattccgac aaccctttaa acgaacatta cagaaacaat
     2941 aacgatatta tcaaacgtga catgttatat aattccaaaa tagactcctt tatgagtaaa
     3001 actccagata tggataaata ctatcttatt gtacccgttt ttttcagaaa cagtataacg
     3061 gcactctttt ttttaacaca aaaagttcgg cgaacccctt actttaaatc agaatacgaa
     3121 tatatctcaa cagccggatc aatagctttt cagacactcg aaaacgagtc tcttattcac
     3181 caatcaataa tcaaaagccg atacgaacag gatcttgata acgcatctta tgttcagatg
     3241 cgccttttcc ctgtctatgt gccttccgaa actggattag agctatcttt atatacccgc
     3301 ccatacaaca aggttactgg agattacctt gatgtcgtag aaattgacga attcagaaca
     3361 gcaatcatga tcggagacat taccggacac ggactaccgg ctgccatgct tcactctacc
     3421 acatccgtac ttattactgc tcttttcaaa cagggcaaaa cactaacaga aattttttat
     3481 gctattaacg attttttgat aaataagtat ttggggcacg agctgataac catgtttgga
     3541 gcgatctttg ataaaagaac atcttcaatg gaatatgtaa acgctgggca tcctccggta
     3601 tacgtgatcc atcccgaaaa ggagaccttt atgcgtgtag agtcaaaagg acatatcctc
     3661 ggtgttacgg atactgcagt ttatactcct caaaaattga cactttctca aaaaagccaa
     3721 cttttctttt ttacagacgg gctcaccgaa ataaaaaaag aaaaagttga ttcaaatatt
     3781 ggcgaagaac atctcgaaaa aatgctgaaa aacatgttaa aagaatctgt agaaaagaag
     3841 atggaagggc tgacctattt tatcagtaaa gtcaaagccg attgcataag tgatgatatt
     3901 acaatcgcgg gagtggagat tagctaaaaa ttcagggccg ccaaacggca gccctaatta
     3961 acaacaactc aatactattc gatgattatc cttctatgat agcatcaacc ggacaaacac
     4021 tagcacaaag accgcaactt gtgcatgcac tttcgtcgat ttcgtactga gaatcaccct
     4081 gagaaatagc ctctacaggg cattcgcttt cacaagatcc gcaagaagta catgaatctg
     4141 ttattttata agccatgtaa gttctcctta atcaatatta ttaatgcgac atagcctctt
     4201 aaaaagctat tagtcaagaa tttattatcg gtaaaataca ataattagta ttaataaccg
     4261 ctttagcttt taaatatcta gaaatcagtt atacacagca aacagttaca agtcagactt
     4321 aatcacatgc cgactccttt tcactctt
//
LOCUS       DAOTGZ010000227         4335 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_3064
            NODE_24057_length_4335_cov_3.415654, whole genome shotgun sequence.
ACCESSION   DAOTGZ010000227 DAOTGZ010000000
VERSION     DAOTGZ010000227.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868088
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 4335)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4335)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:22:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,785
            CDSs (total)                      :: 2,751
            Genes (coding)                    :: 2,732
            CDSs (with protein)               :: 2,732
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 12 of 19
            Pseudo Genes (internal stop)      :: 5 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4335
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_24057_length_4335_cov_3.415654"
                     /isolate="AMR_MDS_3064"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..2301
                     /gene="secA"
                     /locus_tag="P5123_11865"
     CDS             <1..2301
                     /gene="secA"
                     /locus_tag="P5123_11865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011670335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006605 - protein targeting [Evidence IEA];
                     GO_process: GO:0006886 - intracellular protein transport
                     [Evidence IEA];
                     GO_process: GO:0017038 - protein import [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecA"
                     /protein_id="HRX17001.1"
                     /translation="EFLGLSVGIIQHNMDHEARKEAYGADITYGTNNEFGFDYLRDNM
                     VQHRSLKVQRELNYCIVDEVDSILIDEARTPLIISGSIEESTKKYYEIDKIIPRLKKD
                     KDYEVNEKDRNVSLTEEGVHSVEHLLGIDNLFDNKNFETVHYVNQALKAHTLFEADVD
                     YIVKDDKVVIVDEFTGRLMEGRRFSDGLHQALEAKEKVTIARESQTLASITFQNFFRM
                     YNKLSGMTGTADTEAVEFHSIYKLDVVVIPTNKPMRRIDHPDMIFRSEREKFNAIAED
                     IRTCSAKGQPILVGTVSIEKSEVLSAMLKNKGIGHNVLNAKFHEREAQIVSEAGSPGM
                     VTIATNMAGRGTDIVLGGSKKHVDEMELYQPVHDEKLWDEFKVLILSNRFDDADNFIN
                     SLEGKDKHKAEAIIRGGREWLVSHKKVLDAGGLYILGTERHEARRIDNQLRGRSGRQG
                     DPGVSRFYLSLEDNLMRIFGSERIYKVMDRLGMEEGQQIESKMVSNAIAGAQKRVEGR
                     NYEIRKHLLEYDDVMNSQRKFIYSLRNEILEGEDISETIKDYMYEAIEDAVETHSMGK
                     GFSDEWDMDSLVSFIKSKFIPDFSFNPKDRKKISFNDFVEETFEKFEAIYKSKAEKIG
                     VENMRQLERMVSLKIIDTKWREHLYQMDELRDGIWTVGYGNKQPLVEYKLKGYDIFQS
                     MVAALKADIAEFLLKVQITESIREEAPAQYKPVGSEHHEEVKQFGAGAGIPTGQSQHR
                     NPNKNRTKSEPVVGGSKRVKSRRNRR"
     gene            2304..3665
                     /gene="glmM"
                     /locus_tag="P5123_11870"
     CDS             2304..3665
                     /gene="glmM"
                     /locus_tag="P5123_11870"
                     /EC_number="5.4.2.10"
                     /inference="COORDINATES: protein motif:HMM:TIGR03990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008966 - phosphoglucosamine mutase
                     activity [Evidence IEA];
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglucosamine mutase"
                     /protein_id="HRX17002.1"
                     /translation="MASELMKSVSGIRGIVGETMTPELVLNIARAFAQYVHCGKVVIG
                     RDGRCTGHAISAIAESVLTMYGCDVVDLGVVPTPTVQIMVEELDAAGGIIISASHNPI
                     EWNAFKLVKNDGTFLNSREIEHFFDILEKGSNRPKVWDEVGQSYTTDNAFTSHIRRVV
                     NNVSVSTIKRRRFKVALDSVNASGSYITAELLNYLNCKTVPVYCEITGTFPRVAEPQA
                     QNLSDLSEAVLRYKCDVGFAQDPDADRLALIDEMGAPLSEEYTLALVTEHLLSKMKGR
                     VVTNLSTTKAVEDIAAKYGVECVRTPVGEIHVVEEMRRGARIGGEGNGGIISPDVHLG
                     RDSLAGIAYILDMMAERKKTLSELFDELPRYHMRKGKVSFSRAKLKGLYAKIKKTFKN
                     EKISDIDGIRIDFKTIEKFENGWVHLRPSNTEPVFRIIAEADTIEKCEAIYNYCGRLI
                     KSL"
     gene            complement(3835..>4335)
                     /locus_tag="P5123_11875"
     CDS             complement(3835..>4335)
                     /locus_tag="P5123_11875"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRX17003.1"
                     /translation="YMQIIVYCSKLAKERKMGYIRFNLPDFHPVYKIAENLGATCDQK
                     YAWQIKIDTVRFLNKISPVLETRLQGTFLEKESFTVKINLYRYYLEIDITKGKIRSII
                     RKPHSGEFDEMFALRECDFYSLITGYESVNEIDKRAHDFFINGYNRSIAEVLFPKQKA
                     FLYYNM"
BASE COUNT         1324 a          768 c          989 g         1254 t
ORIGIN      
        1 gaatttttgg gattatccgt tggcattatt caacataaca tggatcatga agcccgcaaa
       61 gaagcatatg gcgctgatat tacctatgga acaaataacg aatttggatt tgattatctg
      121 cgtgacaata tggttcagca tcgttcactc aaggtgcagc gtgagctgaa ttactgtata
      181 gtcgatgagg ttgactcgat acttatagat gaggctcgta caccacttat aatttccggt
      241 tcaattgaag agagcaccaa gaagtattac gaaatagaca agataattcc gcgtcttaaa
      301 aaagacaagg attacgaggt aaacgaaaaa gaccgtaatg tctcgttaac agaagagggt
      361 gtgcactcgg tagaacatct cctgggtatc gacaaccttt ttgataataa aaattttgaa
      421 accgttcact atgtgaacca ggccttgaag gctcatactc tttttgaggc agatgtcgac
      481 tatattgtaa aagacgacaa ggttgtcatc gttgatgagt ttaccggacg tctgatggaa
      541 ggacggcgtt tttctgacgg acttcatcag gctttagagg caaaagagaa agttacaata
      601 gctcgtgaaa gccagaccct cgcctcgatt acttttcaga actttttccg tatgtacaat
      661 aaactgtccg gtatgacagg aactgccgat accgaagcgg tagaattcca cagtatatac
      721 aaacttgatg tagttgttat accgactaat aaacctatgc gaagaataga tcaccccgat
      781 atgattttca gatccgagcg tgaaaagttt aacgcaatcg cagaagatat acgaacctgt
      841 agcgcgaagg ggcaaccgat tcttgttggt acagtctcta ttgaaaaatc tgaagtgctt
      901 tcggcaatgc ttaaaaataa gggtattggt cataatgtgt tgaatgccaa gttccacgag
      961 cgtgaagcac aaatcgtatc cgaagcgggt agtcccggaa tggttacaat tgcaaccaat
     1021 atggcaggac gtggaacgga tattgtgctt ggtggaagta aaaaacatgt cgatgaaatg
     1081 gaactgtatc agccagtaca tgatgagaag ctttgggatg aattcaaggt attgattcta
     1141 tcgaaccgtt ttgatgatgc agataatttt attaattctc ttgaaggcaa ggataagcat
     1201 aaggccgagg caataattcg cggtggaaga gagtggcttg ttagccacaa aaaggttctt
     1261 gatgcaggtg gcctttatat tctaggaacg gaacgtcatg aagcgcgccg tatcgataat
     1321 cagctaagag gtcgatcagg gcgtcaggga gatcctggtg tctcacgttt ctatctttca
     1381 cttgaagata accttatgcg tatttttggt tcagagcgta tttacaaggt tatggatcgt
     1441 cttgggatgg aagaaggcca gcagatcgaa agcaaaatgg tttctaacgc gattgctgga
     1501 gcacagaaac gtgtcgaagg tcgtaactac gaaatacgta aacatcttct tgaatatgat
     1561 gatgttatga attcacagcg taaatttatc tactctttgc gtaatgaaat tcttgaaggt
     1621 gaagatattt cggaaacaat taaggattat atgtacgagg cgattgagga tgctgtagag
     1681 actcattcta tgggcaaggg attctctgat gagtgggaca tggattcact tgtgtctttt
     1741 attaaatcca agtttattcc tgatttttct tttaatccaa aagatcggaa aaagatcagt
     1801 tttaacgatt ttgttgaaga aacatttgaa aaatttgagg ctatttataa gagcaaggcc
     1861 gagaagattg gtgtagaaaa tatgcgccaa cttgaacgta tggtctcttt gaaaattatt
     1921 gatacaaagt ggcgagagca tctttatcag atggatgagc tacgtgacgg aatttggaca
     1981 gtaggttacg gaaacaagca acctcttgtg gagtacaagc ttaagggcta tgatattttt
     2041 cagtcaatgg tagctgcgtt aaaggccgat atagccgagt ttcttcttaa agttcagatt
     2101 acagaatcaa ttcgagaaga ggctcccgct cagtataagc ctgttggcag tgaacatcat
     2161 gaagaggtca agcagtttgg tgcaggtgcc ggtatcccga cagggcagtc gcaacaccgc
     2221 aatccgaata agaaccggac taaatccgaa cctgtagtcg gtggttcgaa aagggtcaaa
     2281 tcccgccgta acaggagata ataatggctt ctgaattgat gaagagcgtt tcaggtataa
     2341 ggggaattgt gggtgaaacc atgacccccg aactagtgct aaatattgct cgtgccttcg
     2401 cgcaatatgt tcattgcgga aaggtcgtta ttggtcgaga tggaagatgt accggacacg
     2461 ctatatctgc gatagcagag tcggtattga ctatgtatgg atgtgatgtt gtcgatttgg
     2521 gagtagttcc aaccccgact gtacagatta tggttgaaga acttgatgct gccggaggaa
     2581 taattatttc ggcttcacat aatccaattg agtggaatgc ctttaaatta gtcaagaacg
     2641 acggcacgtt tttaaacagt cgtgaaatag agcatttttt tgatatactg gaaaaaggca
     2701 gtaaccgtcc caaagtttgg gatgaggtag gtcaatcata tacaactgat aacgcattta
     2761 cctctcatat tagaagagtt gtaaataatg tctctgtgtc aacgatcaag cgacgccgtt
     2821 tcaaggtagc ccttgattct gtaaatgctt cggggtcgta cattacagct gaactgttaa
     2881 attatcttaa ctgtaaaacc gtgcctgtat attgtgagat tacgggtacc tttccccgtg
     2941 tggccgaacc acaggctcaa aacttgtctg atctgtcaga agcagttcta agatacaagt
     3001 gtgatgtcgg ttttgctcag gatcctgacg ccgaccgtct ggcgctgatt gacgaaatgg
     3061 gagctccgct atctgaagag tacacacttg cccttgtgac ggaacacctg ttatcaaaaa
     3121 tgaagggccg cgttgtgact aatctttcta caaccaaagc agtagaagat atagcagcaa
     3181 aatatggtgt agagtgtgtg cgtacaccgg ttggtgagat tcatgtggtc gaagagatgc
     3241 gacgaggtgc acgaatcgga ggggagggaa acggtggtat tatctctcct gatgttcatt
     3301 tggggcgtga tagcctcgcc ggtatagctt atattttgga tatgatggca gagcggaaaa
     3361 agactctttc agagcttttt gatgagctac cccgttatca tatgcgcaag gggaaggtct
     3421 cttttagccg agccaaattg aaagggcttt acgcaaaaat taaaaagaca tttaaaaatg
     3481 agaagatatc agatattgac ggaataagaa tagattttaa aactattgag aaatttgaaa
     3541 atggctgggt tcatttaagg ccatccaata ccgagcctgt tttcagaata atagctgaag
     3601 ccgatacaat agaaaaatgt gaagccatct ataactattg cggccgtttg atcaagagtc
     3661 tgtagttctt tttaacaaca tcctctccta ataataagat aatttttccc gttattactc
     3721 gaaaaaccgc taaaataaag cactttaact aaagtgcttt attttagctt aattaaaaac
     3781 ggtaaccata ttttttaatt cacagtttcc gttaacttga gcagttaaaa gttattacat
     3841 attgtagtac aaaaatgctt tttgcttagg gaaaagaact tctgctattg aacggttgta
     3901 tccgttaata aaaaagtcat gcgctctttt atcgatttca ttgaccgact catatcctgt
     3961 aattaaacta taaaaatcac actctcttag ggcaaacatt tcatcgaatt ctccagagtg
     4021 aggttttctt ataatagatc taattttacc tttggtgata tcaatttcga gatagtaccg
     4081 atagagatta atcttaacag taaaactttc tttttctaaa aaagtccctt gtaatctagt
     4141 ctctaaaacc ggagagattt tgttcaaaaa ccttacagta tctattttga tttgccatgc
     4201 atacttttga tcacatgtag ctccgagatt ttctgctatc ttgtataccg gatgaaaatc
     4261 gggtaggttg aacctgatat atcccatttt cctctctttg gccaatttgc tgcaataaac
     4321 aataatttgc atgta
//
LOCUS       DAOTGZ010000228         4221 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_3064
            NODE_24890_length_4221_cov_3.790927, whole genome shotgun sequence.
ACCESSION   DAOTGZ010000228 DAOTGZ010000000
VERSION     DAOTGZ010000228.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868088
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 4221)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4221)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:22:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,785
            CDSs (total)                      :: 2,751
            Genes (coding)                    :: 2,732
            CDSs (with protein)               :: 2,732
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 12 of 19
            Pseudo Genes (internal stop)      :: 5 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4221
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_24890_length_4221_cov_3.790927"
                     /isolate="AMR_MDS_3064"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(282..413)
                     /locus_tag="P5123_11880"
     CDS             complement(282..413)
                     /locus_tag="P5123_11880"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRX17004.1"
                     /translation="MLGMTIISPAVMIGIVVTFCLVFAAVVSLVHDNWKPRDPHHRH"
     gene            complement(495..722)
                     /locus_tag="P5123_11885"
     CDS             complement(495..722)
                     /locus_tag="P5123_11885"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRX17005.1"
                     /translation="MPYNRQTDINDLITAKACVADLLLAVNRLTKVYDTFVGDSVEMR
                     VKYNKESSKIIKFPSTPDFETRNTKKKEKNK"
     gene            complement(869..1045)
                     /locus_tag="P5123_11890"
     CDS             complement(869..1045)
                     /locus_tag="P5123_11890"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRX17006.1"
                     /translation="MFYYNEQILKDYRDSNIKCRMDLYEKYPYMREIFNSIDLEECAA
                     RKIEKLKKEKVATV"
     gene            complement(1179..2063)
                     /gene="folD"
                     /locus_tag="P5123_11895"
     CDS             complement(1179..2063)
                     /gene="folD"
                     /locus_tag="P5123_11895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012507969.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004488 - methylenetetrahydrofolate
                     dehydrogenase (NADP+) activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional methylenetetrahydrofolate
                     dehydrogenase/methenyltetrahydrofolate cyclohydrolase
                     FolD"
                     /protein_id="HRX17007.1"
                     /translation="MSANIIDGKQIATEIRSKLAEEIISLSSHGVPGLAVVLIGNNPA
                     SEVYVRMKKKACSDIGVNSYEYSFGADYGEENLLKLIDELNNDSKINGILVQLPLPKG
                     YDEKKVIEAISPLKDVDGFHPENLGRIVIGDRDCFQPCTPYGCQIMIKRTVKDLKGKH
                     VVIVGRSNIVGKPLANMLVQKNDMANCIVTVCHSASEDISKFTLQADILVAAAGSPGL
                     ITADMVKPGAVVIDVGTNRVDDPENPDKCKLVGDVDFEAVKEVAAAISPVPGGVGPMT
                     ITMLLENTVKAFRLHNDI"
     gene            complement(2065..3057)
                     /gene="amrS"
                     /locus_tag="P5123_11900"
     CDS             complement(2065..3057)
                     /gene="amrS"
                     /locus_tag="P5123_11900"
                     /inference="COORDINATES: protein motif:HMM:TIGR04337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AmmeMemoRadiSam system radical SAM enzyme"
                     /protein_id="HRX17008.1"
                     /translation="MSEPALYFSKNNSKYICDLCPHSCVLTDSAESFCRVRGLIGGKP
                     SLLSYGMISSIAVDPIEKKPLYHFYPGSQAFSIGSYGCNLRCPYCQNWQISQSFTNSS
                     IVSPRKLVDMALESGAPSIAYTYSEPLIWYEYIYDCAQLAKEAGLNNILVTNGYIHQK
                     PFEKLLPHIDALNIDLKSFNKNTYKSILKGELEVVLENIKMAVNYAHVELTTLVVTGM
                     NDDMSEMQDACRWIASLDKNMVWHVSRYFPSFQYNKKSTDVSFIEELYVFARSILAHV
                     YTGNMHAENGMQNSYCASCGKVLIDRNGYSVRLVSLKNGRCDACGHPFEAVLEV"
     gene            complement(3059..3454)
                     /locus_tag="P5123_11905"
     CDS             complement(3059..3454)
                     /locus_tag="P5123_11905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002770691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polymer-forming cytoskeletal protein"
                     /protein_id="HRX17009.1"
                     /translation="MAKKIIQINKNPVYDFGMVATVFGADTEFYGDLNYKQSLQINGY
                     FEGEIVSDGFLVIGKGAMVKANVKAKSVIVSGEIHGNIEATDNIEIHENGKLYGNIRT
                     TKLKIADGVVFEGNCEMIKPDGEIKKAAV"
     gene            complement(3467..3688)
                     /locus_tag="P5123_11910"
     CDS             complement(3467..3688)
                     /locus_tag="P5123_11910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010575394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HRX17010.1"
                     /translation="MVSEQKRAVLGHYNEGLSFYRNRKWDEAMAEFTKALEIDAADGP
                     SECYLKRCREYKKNPPPPDWDGVFTMTTK"
     gene            complement(3700..4029)
                     /locus_tag="P5123_11915"
     CDS             complement(3700..4029)
                     /locus_tag="P5123_11915"
                     /inference="COORDINATES: protein motif:HMM:TIGR00377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043856 - anti-sigma factor antagonist
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="STAS domain-containing protein"
                     /protein_id="HRX17011.1"
                     /translation="MKRISEENMEIIELDGRIDQEASEELENTLEECLSGGNTNICID
                     MINVKHICSSALGVLVAVKRKIKDNHGDIKLVIANDNLMKLFQTTMLDKVFEIYETQR
                     ECHTAFD"
     gene            complement(4063..>4221)
                     /locus_tag="P5123_11920"
     CDS             complement(4063..>4221)
                     /locus_tag="P5123_11920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009538735.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosyltransferase family protein"
                     /protein_id="HRX17012.1"
                     /translation="YKPYKKVNCIGKTVLLVDDVVTTGATLNECARIIKKMGANAVFS
                     LTIARVNI"
BASE COUNT         1324 a          903 c          666 g         1328 t
ORIGIN      
        1 aacagaaaac gaattgaaga tctggtgtaa ggataatata tctaattaca aaatccctcg
       61 agcttttatc atagaaaaac aataaagtca ggcaattaat aaagtcttgt tctaatgctt
      121 tattaaaaca gctttataga atttaattaa caggtatcag gccaatactg caaaaataca
      181 aaaagggtct cccgcaaagg agaccctttt tgtatgttat agtaatatta acctttacat
      241 gaacaaataa ctaacctgcc attcaaaact ggtcatctat tttagtgtct gtgatgagga
      301 tcacgcggct tccaattatc gtgaaccaaa gatacaacag ccgcaaaaac aagacagaac
      361 gtaacaacta tccctatcat aacagccgga ctaataatcg tcattccaag cataaacacc
      421 tcctctatcc gatatacgga aaagtaaatg ctatactatt aatataataa acattcgtaa
      481 tatgtcactt tagattactt atttttttct tttttctttg tattccgtgt ttcaaaatca
      541 ggagtagagg ggaactttat aatttttgac gattccttgt tatattttac ccgcatctca
      601 accgagtccc ctacgaaagt atcataaacc ttggttaatc tgtttacggc caacaaaaga
      661 tccgcaacgc aggccttcgc ggttatcaaa tcattaatat ccgtctgtct gttataaggc
      721 acaacacacc tcttattaca gggagtagaa aaaatttctt caaacttttc ggatataaat
      781 ataacaaaaa agaattaaat gtgtatctaa aatataagaa aatgtctttt ttttataatt
      841 ttggcaggtt aactatgaaa gaagcaagct atactgtggc aactttctcc ttttttaact
      901 tttcaatctt tcgagctgca cactcttcaa gatctatact gttaaaaatt tcacgcatgt
      961 atggatattt ttcataaaga tccatgcggc acttaatatt tgagtctcga tagtccttta
     1021 atatttgctc attgtaataa aacatataaa accccattct tcgataaaat aaccacttaa
     1081 tgtctattcg gaagcaatgg ggatttatta tagtggtaaa ttaaggaaac ctcgaataat
     1141 taacttttta acagtcgtaa cttgaaatta ctactttttt atatgtcatt atgcaatcga
     1201 aaggctttaa cagtattttc aagcaacatt gtaattgtca tcggccccac tccgcctggc
     1261 accggcgaaa tagcagcagc aacttcttta actgcctcga aatcaacatc cccgaccaat
     1321 ttacatttgt ccggattttc cggatcatcc acccgattcg ttccaacatc tataacaaca
     1381 gccccaggtt taaccatatc agctgtaatc aaacctggcg agcctgcagc agccacaagg
     1441 atatcagctt gcaaagtaaa ttttgaaata tcttcagaag cactatgaca aacggtaaca
     1501 atacaatttg ccatatcgtt tttttgaacc agcatatttg caagaggttt acctacaata
     1561 ttactccttc ctactataac aacatgctta cctttcaggt ccttaacagt tcgttttatc
     1621 attatttgac aaccatacgg ggtacaaggt tgaaaacaat ccctatcacc gataactatt
     1681 cgacccaagt tttcaggatg aaagccatct acatccttta gaggactaat agcttcaatt
     1741 actttcttct catcatatcc cttaggtaaa ggaagttgaa ctagaattcc attaattttt
     1801 gaatcattat ttaattcatc aattagcttt agtaaatttt cttcgccata atccgctcca
     1861 aaagagtact cgtacgagtt cacaccaata tctgagcatg ccttcttctt catgcgcaca
     1921 taaacttctg acgcgggatt attaccaatt aaaactactg caagacctgg aacgccatgc
     1981 gaagagagag agattatctc ttccgccagt tttgaacgga tttccgtagc gatctgtttt
     2041 ccgtctatta tatttgcact cacattaaac ctccaaaacc gcttcaaagg gatgcccaca
     2101 ggcatcacag cggccgttct ttaacgagac aagcctaaca ctgtagccat ttctgtcaat
     2161 cagcactttt ccgcaagaag cacagtaaga gttttgcatt ccgttctcgg catgcatgtt
     2221 tcctgtataa acatgcgcca atattgaacg tgcaaaaaca taaagttctt caatgaaaga
     2281 gacatctgtg ctttttttgt tgtattgaaa cgatggaaaa tagcgagaaa catgccagac
     2341 catattttta tccagcgaag ctatccacct acatgcatct tgcatttcgc tcatgtcgtc
     2401 attcatacca gttactacaa gtgttgtcag ctcgacatga gcatagttaa ctgccatttt
     2461 gatattttct aaaacaactt caagttcgcc ttttagaata ctcttatatg tgtttttatt
     2521 aaagcttttt aagtcaatgt ttagtgcgtc aatatgtggt aaaagtttct caaacggttt
     2581 ttgatgaatg taaccatttg taaccagaat gttattaagc ccagcctctt tagccaattg
     2641 agcacagtca tatatgtact cataccatat aagtggttca gagtatgtat atgctataga
     2701 aggagctcct gattcaagag ccatatcaac cagtttgcgt ggtgaaacaa tagaagaatt
     2761 ggtaaaactt tgagatattt gccagttctg acaatatggg caacgcaaat tacaaccgta
     2821 actgccgata gaaaaagcct gggaacccgg ataaaaatga tacaaaggtt ttttttcaat
     2881 gggatcaaca gcgatcgagg agatcattcc ataggataaa agggacggtt taccacctat
     2941 aagcccccga acccggcaga atgattcagc cgaatccgtt aaaacacagg aatggggaca
     3001 taagtcacag atgtatttac tattgttttt gctaaagtag agagcaggtt ctgacatttt
     3061 aaacagccgc tttcttaatt tctccgtccg gtttaatcat ctcacagttg ccttcgaaaa
     3121 caacaccatc agcaattttt aattttgtcg tgcgaatatt tccataaagt tttccatttt
     3181 catgaatctc tatattatca gtggcctcta tatttccatg aatttctccc gaaacaatca
     3241 cagactttgc tttaacattt gccttaacca tagccccctt gccaatgaca agaaaaccgt
     3301 cagaaacgat ctcaccttca aaatatccat tgatttgcaa agattgctta tagtttagat
     3361 ctccgtaaaa ttccgtatcg gctccaaaaa cagtagctac cattccaaaa tcgtataccg
     3421 gatttttgtt tatctgaatt atcttttttg ccatcaaaaa aactccctat tttgtagtca
     3481 ttgtaaaaac tccgtcccag tctggtggag gcggattttt tttgtactca cgacatcgct
     3541 ttaaatagca ttccgaagga ccgtcagccg catcaatttc aagtgccttg gtaaattcgg
     3601 ccatagcctc atcccatttg cgattgcgat aaaaagaaag accttcgtta taatggccaa
     3661 gaacagctct tttttgttcg gataccattg tccctccctc taatcaaaag ctgtgtgaca
     3721 ctcgcgttga gtctcgtata tttcaaacac cttgtcaagc atggtagttt ggaaaagttt
     3781 cataagatta tcattggcta ttaccaattt gatatcgcca tggttgtcct tgattttacg
     3841 cttaacagct acaagaacac ccagcgctga actgcatata tgcttaacat tgatcatatc
     3901 aatacatata tttgtgttgc ctcccgacaa acactcctca agagtattct caagttcctc
     3961 agaggcctcc tggtcgattc ttccgtccag ttcgattatt tccatatttt cttcagaaat
     4021 gcgtttcatt aattactccg ttataactag tcatggatat aattatatat tcacacgagc
     4081 aatggtcaat gaaaatactg cattggcacc catttttttt ataattcttg cgcactcatt
     4141 gagcgtggct cctgtcgtga ccacatcatc aacaagcaaa actgttttcc caatacaatt
     4201 aactttttta taaggtttat a
//
LOCUS       DAOTGZ010000229         4219 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_3064
            NODE_24900_length_4219_cov_5.350865, whole genome shotgun sequence.
ACCESSION   DAOTGZ010000229 DAOTGZ010000000
VERSION     DAOTGZ010000229.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868088
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 4219)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4219)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:22:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,785
            CDSs (total)                      :: 2,751
            Genes (coding)                    :: 2,732
            CDSs (with protein)               :: 2,732
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 12 of 19
            Pseudo Genes (internal stop)      :: 5 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4219
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_24900_length_4219_cov_5.350865"
                     /isolate="AMR_MDS_3064"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1008)
                     /locus_tag="P5123_11925"
     CDS             complement(<1..1008)
                     /locus_tag="P5123_11925"
                     /inference="COORDINATES: protein motif:HMM:TIGR01883.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M20/M25/M40 family metallo-hydrolase"
                     /protein_id="HRX17013.1"
                     /translation="MYQVNEKRLYKIFKDLASISSPSMKEEKVIDYLEEFSQKHGLSF
                     TTYPCGKSRNVMMKLKATDVSKKSVLFSAHTDTVTPCENIKVIENETRFTSDGTTVLG
                     GDDKAGIAAVLEAVLYISENKIPHGGLELLFTCAEEIGLVGMKAMDLKIIKSKLCYVL
                     DSGGPIGTAAIQAPYHLFVGTEIKGKAAHAGMEPENGISAITALAGIISKLPSGRIDA
                     ETTTNIGTVSGGKATNIVAADASFALEIRSLSQKKMKALESKIVQTIKNEALKHRVKV
                     KIERNLEYSGFCVKMDSPVIEHFEKACASIKIKPRYEASGGGSDTNVLQSAGLEAVNL
                     SA"
     gene            complement(1184..3688)
                     /locus_tag="P5123_11930"
     CDS             complement(1184..3688)
                     /locus_tag="P5123_11930"
                     /inference="COORDINATES: protein motif:HMM:NF012695.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate nucleotidyltransferase"
                     /protein_id="HRX17014.1"
                     /translation="MIKAVIMAGGEGTRLRPLTSNRAKPMTPVVNKPVIEHAVELLKR
                     HGISDIVISLFYLPENIQNYFGDGSEWDVSITYSVEETPLGTAGGVHLASEGFDDTFI
                     VLSGDGIIDFDLTKIIDFHKQKKSDFTIALKRVKEPTEYGIVITDPVSGKIEKFLEKP
                     SWSEVFSDTANTGVYVIEPGFINKFVQPDKPSDFSFDLFPLFEKQEVSIYGCIVDGYW
                     CDVGNIDSFLSVHRDILNRVADVIIPGKMIDEQVWVGKNVQIEEGVQIQGPVLIGDFV
                     KVKKGAEISDYSIIGNNCVVEARASIRRSVILHSTIIGPGAELRGAVIGKRCFLEENV
                     SIYERAVISDDCHIGAHAEIPSTIRVWPDKVIEEGTRLTTDLIWGRSEKKLLFNNSSI
                     TGSFNVKITPEFSAKLGSAVGAWLGLNTRVIVSRDASGASRLIKRAITSGLLSMGVDV
                     FDLEIESVSVARYASRFIKTDLVINVQTKPQTGLQYIVINFFNKNGFRLNLSAEKKIE
                     GIFYRGDYPRKSAYEVGQIHYSTLYKEAYKDYLSSHIDHELLKSSQMPIIIDCRYGAS
                     SYILPDVLSFFGLKTTVLRGQIQYGDIGIELKNENINERGALSSMCASNGEVGAIVSS
                     TGSNIFVYDEHGDLLSEDEMIMLLSIFFASYRDARVFLLPVTASRKLELILSGFGAKV
                     KRISTKIRSPKKTTQIYSVSEEGVYPSLIIENDIIVTFLMLLELVLKQKKSLAQLRRE
                     LLEINMIHKRIDCTQNEKAQIMRAVTEASVKESVELTDGIRIVREDCWILLLPDGSQP
                     FIHVYAEGNTEKLRDSILSEYTRMIKKFSGSNSRYV"
     gene            complement(3894..>4219)
                     /locus_tag="P5123_11935"
     CDS             complement(3894..>4219)
                     /locus_tag="P5123_11935"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRX17015.1"
                     /translation="HLATSIGCICEQQESSCDQHRLHMRAARVILRVSIGCPAPFFKE
                     NGAGRMSKGTCPCRLFLSDNKLLAQDKSPTGNTKNSFKKLKFEAVLKAKNFSGISSDN
                     EKYCP"
BASE COUNT         1277 a          907 c          746 g         1289 t
ORIGIN      
        1 tgctgacaga ttaacagcct caaggcctgc cgattgtaaa acattggtat cagaaccacc
       61 acccgaagcc tcataacgag gctttatctt tatactcgcg cacgcttttt cgaaatgttc
      121 tattactgga gaatccattt tcacacaaaa acctgaatac tcaagatttc tttctatttt
      181 tacttttact ctgtgtttaa gcgcttcgtt ttttattgtc tgtacaattt tactttcaag
      241 tgccttcatc tttttttgag aaagcgacct tatttcgagt gcaaacgaag catcagccgc
      301 aactatattt gtggctttac caccactgac tgtaccaata tttgtggttg tctctgcatc
      361 aatacgtccc gaaggcaact tggaaattat accggctaaa gccgttatcg ccgaaatgcc
      421 attttctggt tccatacctg catgagcagc cttgcccttt atctcggttc caacaaaaag
      481 gtgataagga gcctgtattg ccgcggttcc aataggacca ccgctatcaa gcacataaca
      541 tagtttggat ttaataattt tcagatccat ggccttcatg ccaaccagac cgatctcttc
      601 ggcacaagta aaaagcagct caagaccacc atgcggaatt ttattttcag atatgtataa
      661 tacagcctca agcacagcag cgatacctgc cttatcatca ccgccaagca ctgttgttcc
      721 atcacttgta aaacgcgttt cgttttcgat tactttaata ttctcacaag gagttactgt
      781 atcggtgtgt gccgagaaaa gcaccgattt ttttgaaaca tccgttgcct ttaacttcat
      841 cattacgttt cgtgattttc cgcagggata tgttgtaaaa cttaatccat gcttttgaga
      901 aaactcctca agataatcaa tgactttttc ttctttcatc gaaggtgaag agatagaagc
      961 aagatcttta aatattttat aaagacgttt ttcatttacc tgatacatta ttttttcctt
     1021 aaagaacctg tttatatgtt ttaaacggca taaccgctct agaaatggta atttttcgaa
     1081 gttccattat aactaaaatt ctttagacgt tatctgatcc aaaagtactt tttttgaaac
     1141 aatgtcaatc gaaactacca tttaaacaaa atttaattgt taactaaaca taacggctgt
     1201 ttgaaccgga gaacttcttt atcatacggg tatattcgga tagaatggaa tcacgaagtt
     1261 tctcagtgtt tccctccgcg taaacatgta taaacggctg tgaaccatcc ggcaataata
     1321 gaatccagca atcttcgcga acaattctta tgccgtcggt caattctact gattctttaa
     1381 cagaagcctc agttacagct cgcattattt gagctttctc gttttgtgta caatcgatac
     1441 gcttgtgaat catatttatt tcaagaagct cccttcttaa ttgagcgaga gactttttct
     1501 gtttaagtac aagctctaac aacattagaa acgtaacaat aatatcattt tcaattataa
     1561 gtgaagggta aaccccctct tctgacacag aatatatctg agtggttttt ttgggcgatc
     1621 gtattttggt tgaaattctt ttaacttttg ctccgaaacc cgaaagaatc aactcaagtt
     1681 ttcgagatgc tgtaaccgga agaagaaaga cacgggcatc gcggtaactt gcgaaaaata
     1741 ttgacaacag catgatcatc tcgtcttcgg aaagaagatc accatgttcg tcataaacaa
     1801 atatattact gccggtagat gatacgatgg caccgacctc accatttgaa gcgcacatcg
     1861 agctcaaagc tccacgttca ttaatattct catttttaag ttctatacca atatcaccgt
     1921 actgaatctg tccgcgcaat actgtagttt ttaatccaaa gaatgaaagc acatcgggca
     1981 aaatatagga tgaagctccg taacgacaat caataataat aggcatttga gacgacttta
     2041 ataattcatg atcgatatgg cttgacaaat aatctttata tgcctcttta tatagggtcg
     2101 aatagtgtat ctgaccaacc tcatacgcag attttcgggg ataatcgcct cggtaaaaga
     2161 ttccctcgat ctttttttct gcagacaaat taagtctgaa gccgttcttg ttaaaaaaat
     2221 taattacgat atattgaagg cctgtttgcg gttttgtttg aacatttatt acaagatccg
     2281 ttttaataaa ccgggaagca taacgtgcca cagagacaga ttcaatttca agatcaaaga
     2341 catcgacacc catagataaa agaccagagg taattgcccg tttgattaaa cgtgaggcac
     2401 ctgaagcatc acggctgaca ataactcgag tgtttaatcc caaccaggct cctacagccg
     2461 agccaagttt tgcggaaaac tcgggtgtga ttttaacatt aaatgagccg gtaatagaag
     2521 agttattaaa aagtaatttt ttttcagaac gcccccagat aagatcagtc gttaatcttg
     2581 taccctcttc tataaccttg tcgggccaga ctcttatagt cgaggggatc tcggcatgag
     2641 ctccgatatg gcaatcatcg cttataaccg ccctttcgta tattgataca ttttcttcaa
     2701 gaaagcagcg tttgccaata acagcaccgc ggagttcggc accaggaccg attattgtac
     2761 tatggagtat aacggatcta cgaatagagg ccctggcttc aacaacacag ttattaccta
     2821 tgattgagta atcagaaatt tccgcgcctt ttttaacttt aacaaaatca cctatcaata
     2881 cgggaccttg tatctgaact ccttcttcga tctgaacatt tttacctacc cagacctgct
     2941 catcaatcat ctttcccgga attattacat ccgcaacacg gttcaaaata tcccgatgta
     3001 cggagagaaa agagtcgata ttaccaacat cacaccagta accatctaca atacatccat
     3061 aaatagagac ctcttgcttt tcaaaaagag gaaacaaatc aaatgaaaaa tcggacggct
     3121 tgtcaggctg aacaaactta ttaatgaaac ctggttcgat cacataaaca ccggtatttg
     3181 ctgtatccga aaagacctca ctccatgacg gtttctccaa aaatttttct atttttccac
     3241 ttacgggatc ggttattact ataccatact cggttggctc ttttacgcgt ttaagagcta
     3301 tcgtgaagtc ggattttttt tgtttgtgaa aatcaattat ttttgtaaga tcaaaatcga
     3361 ttattccgtc accagagagc actataaaag tgtcatcaaa accttccgag gcaaggtgca
     3421 ctccgcctgc agtacctaga ggggtctcct cgaccgaata tgttattgag acatcccatt
     3481 ctgagccgtc accaaaatag ttttgaatat tttccggaag ataaaagagg ctaattacga
     3541 tatcgctgat accatgacgc tttagaagct cgacagcatg ttcgatgact ggtttgttta
     3601 cgaccggagt catgggttta gcacggttgg aagttagagg gcgaagccgg gtaccttctc
     3661 cgcccgccat aataacggcc tttatcataa ataatccctt aaaatatttt ttacaggaag
     3721 acaaaacttt attcacaagc agataagcat atattataat atatagctct caaaatgata
     3781 tgtcaaacaa ctttacaata tgaaagacat ataggttaac ctcaaataat aactatttat
     3841 agggaacttc gaaaaattac cattttcgga acggctatgc cgtttaaagc atattaagga
     3901 caatatttct cgttgtcact cgaaataccg ctgaaatttt tagctttaag gacagcttca
     3961 aatttcagct ttttaaagga atttttcgtg ttacccgtgg ggcttttatc ttgtgctaaa
     4021 agcttattgt ctgataagaa caacctacaa gggcaagttc ctttgctcat tcgtccagca
     4081 ccattttctt taaaaaatgg tgctgggcaa cctatgctca ctcgcaagat gactcttgct
     4141 gctcgcatat gcaacctatg ctggtcgcaa gatgactctt gctgctcgca tatgcaacct
     4201 atgctggtcg caagatgac
//
LOCUS       DAOTGZ010000230         4212 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_3064
            NODE_24959_length_4212_cov_6.665143, whole genome shotgun sequence.
ACCESSION   DAOTGZ010000230 DAOTGZ010000000
VERSION     DAOTGZ010000230.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868088
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 4212)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4212)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:22:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,785
            CDSs (total)                      :: 2,751
            Genes (coding)                    :: 2,732
            CDSs (with protein)               :: 2,732
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 12 of 19
            Pseudo Genes (internal stop)      :: 5 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4212
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_24959_length_4212_cov_6.665143"
                     /isolate="AMR_MDS_3064"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(331..510)
                     /locus_tag="P5123_11940"
     CDS             complement(331..510)
                     /locus_tag="P5123_11940"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRX17016.1"
                     /translation="MKNEKPDMILYAKISLKRHRKDARLSVSFDSVKDRFLYKKSLCG
                     KYPKDARSSVSLDAV"
     gene            937..1746
                     /gene="lepB"
                     /locus_tag="P5123_11945"
     CDS             937..1746
                     /gene="lepB"
                     /locus_tag="P5123_11945"
                     /EC_number="3.4.21.89"
                     /inference="COORDINATES: protein motif:HMM:TIGR02227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006465 - signal peptide processing
                     [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="HRX17017.1"
                     /translation="MIKKRNVFISALLALFSPVTAFLYNGKVVFGGILVSVMYLLFYI
                     LLISGIAHNFYGFLIVGLYIIAFTLFIIIDNVVTAIKNKNYELKKYNRVSVYIISTIL
                     FAFITGSANVLINKYFFQLYSIPAGSMAPAIVEGDSVAVDKSFYRKHKIKRGDLVVFL
                     YPNDRSVTYVKRCVAVSGNNLVIDGKDIIINGKKIVENYPLMYNDEDSIFRDIYDYGI
                     VPDNHIYVLGDNRDNSADSRHFGVVPVKDVIGKPLYIYNSKDRSRIGLELK"
     gene            complement(2018..2671)
                     /locus_tag="P5123_11950"
     CDS             complement(2018..2671)
                     /locus_tag="P5123_11950"
                     /inference="COORDINATES: protein motif:HMM:NF024811.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family phosphatase"
                     /protein_id="HRX17018.1"
                     /translation="MKDRGVIFDFNGTLFWDSEYQESSWDEYLKNHNINMTASQKKEY
                     IHGRNGKDTLEYLFKRELSDIEVEQYTEEKEQLYRKECLKHKMELAPGAVDLLNHLNE
                     QKIPIAIATASGKRNVDFFIDKLNLLDYFSEEHIIHNDGKIKGKPHPDIFEKAISRLG
                     IDSSKTIIFEDSISGIQAAMNSKVANIIIVNSTKENYSGFNFPIIEHFDQFDRNLLT"
     gene            complement(2791..2913)
                     /locus_tag="P5123_11955"
     CDS             complement(2791..2913)
                     /locus_tag="P5123_11955"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRX17019.1"
                     /translation="MKYSKEVLDWFDNFRKRITNRIDSFSELSDSAPIKDTLIF"
     gene            complement(3162..3590)
                     /locus_tag="P5123_11960"
     CDS             complement(3162..3590)
                     /locus_tag="P5123_11960"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRX17020.1"
                     /translation="MTYFALSDAGCIVVSEKLKNTLDENQIDNIDYYPATVVLPKEDS
                     PKSGYYAANIKGLVDAIYLDKSECRAIEHKSRTAIVRMEKLVLKDVEVSHSSIYRLKM
                     FRSPIIVEGKLCSYLSENSFSGIKLVAPEKWDGVYGELES"
     gene            3767..3904
                     /locus_tag="P5123_11965"
     CDS             3767..3904
                     /locus_tag="P5123_11965"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRX17021.1"
                     /translation="MKNYQIINWQRFDELALECFKKQIRSIEDLADNGSLDLYDTWHQ
                     L"
     gene            complement(3922..>4212)
                     /locus_tag="P5123_11970"
     CDS             complement(3922..>4212)
                     /locus_tag="P5123_11970"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRX17022.1"
                     /translation="IDPDKRKSYMDYKIVNIVSAIDCIDFEQSKITCYDDGDVEFIDK
                     LVLSEDKIQANMDIFRLARNLETTVFSERLKMAIEKNDITGCVFYRVDKYSI"
BASE COUNT         1281 a          727 c          737 g         1467 t
ORIGIN      
        1 aaccacatag tcaaaacaat tgcgtaatcc tcaagcgcga ttgttttgac ggattcgggc
       61 gaatagcccg aaaatgttcc atgcttatca ccactttcgc ggagtgccag cgacgcgaag
      121 tggtgactgc cacgaggggc tgccgtccct cgaccctgca gggtgaagat tatttatcag
      181 ctttgcaaag gttttttcaa tgtggtttaa ttgataactc ctacttttgg atgttcgcta
      241 agttgacatc ctgtcaaaag cgctacaacg ccggcatcgt gccggcttca gagtacatta
      301 ttttgaagtg ctggttttat ggattttgtt ctacacggca tctagcgaga cgctcgatcg
      361 agcatcttta gggtactttc cacagagact ttttttgtat aaaaacctgt ctttgactga
      421 atcaaaagag acgctcagcc gagcgtcttt acgatgcctt tttagtgata ttttggcata
      481 cagaatcata tctggttttt catttttcat ttttagttgt tcattgcctt atctgtggag
      541 tgataacgac gcgatgcgat aacatcctgt caaaagcgct accgtgccag cgccagttta
      601 tataatttca attgtaggaa gcaagatctg aaaattcgat taggttaagt cgtcatgaga
      661 gacaatctat agaagaacat attgttactt ttaattgtgg cagtcttata aaggtaaaaa
      721 agcccaaaaa agtagatgaa aacgttacgc aatttattca aaaaaaggcg caattttgtt
      781 gatatatacc aacttcagat acacatacat tcagctttat aacatgacat catgcgcaat
      841 gtttcatttg caatattaat aatgattgtg gttttaatta ctatttacaa taattgatat
      901 attagtttca aaataaaatt gtttgttgga gatattatga ttaaaaaacg taatgtattt
      961 atttctgctt tattggcgtt gttttctcct gtaacggcat ttttgtataa cggtaaagta
     1021 gtttttggcg gtattttggt ctcggtaatg tatctactat tttatatatt attaatttca
     1081 ggtattgctc ataattttta tggattttta attgttggat tgtatattat tgcttttact
     1141 ctatttatta taatagataa cgttgtaaca gcaattaaaa ataaaaatta tgagttaaaa
     1201 aaatataaca gagtatcagt ttatataata agtactatat tgtttgcatt tattacaggg
     1261 tctgcaaatg ttttaataaa taaatatttc tttcagcttt attcaatacc tgccggttca
     1321 atggctcctg ctattgttga aggtgacagt gtagcagtag ataagagttt ttacagaaaa
     1381 cataaaatca agagaggtga ccttgttgtt tttttatatc ccaatgatcg ttcagtaact
     1441 tatgtaaagc gatgtgttgc tgtttctggt aataatcttg ttattgatgg aaaagatatt
     1501 attataaatg gcaagaagat agttgaaaat tatcctctta tgtataatga tgaagattca
     1561 atttttaggg atatttatga ttacggtata gttcctgata atcatattta tgttttgggt
     1621 gataaccgtg ataattcggc cgatagcagg catttcggtg ttgtgccggt taaagatgta
     1681 atcggtaaac cgctctatat ttacaattca aaagataggt cgcgaattgg cctggagcta
     1741 aaatagcgta tgtaaaatag tacatttcaa ttaagtttaa tcctgtaaag attgtcgttt
     1801 tgaattgaga tttgacggct tggaattatt tatataaaac tatacatgta accacattgg
     1861 acagaagata tatcttcgaa tttttggaga tggatcgaac gaagtggtcc atccatatta
     1921 ttttttcccg agttgtaaag taatgatttc aggcatgccg gccatttaca tgaaacagta
     1981 aaattttggt aatggtgctg aaattattca tataggtcta agtcagcagg tttctgtcaa
     2041 attggtcaaa atgttcgatt atggggaaat tgaacccgga ataattctct tttgtagaat
     2101 ttacaattat gatattcgca actttactat tcatggcagc ctgaatgcct gagatagagt
     2161 cttcaaatat gatagtcttt gaactgtcta tgcccaatct ggatattgct ttttcaaata
     2221 tatcagggtg aggttttcct tttattttcc cgtcgttatg aattatatgt tcttcagaga
     2281 agtaatccag taaattcagt ttatcaataa aaaaatccac gttccttttc cctgatgctg
     2341 ttgcgatcgc gatcgggatt ttctgttcat ttaaatggtt taaaaggtct acggctcccg
     2401 gcgcaagttc cattttatgt ttaaggcact cttttctgta cagctgctcc ttttcttctg
     2461 tatattgttc aacttctata tcactcagtt cccgtttaaa aagatattca agcgtatcct
     2521 ttccgtttcg cccatgaata tactctttct tttgagatgc cgtcatatta atgttatgat
     2581 tctttaaata ttcatcccac gagctctcct gatattcgga atcccaaaac aaagtaccat
     2641 taaaatcaaa aattactcct ctgtctttca tgacgctctt cccttgaaaa gaatttacct
     2701 gtcgttaagc atagttatat aaacataata atatttccag aaaaaaatag tattatataa
     2761 gactcattta gaatcttaaa tgcaaattaa ttaaaatata agtgtatcct ttattggtgc
     2821 tgagtcggac agctccgaga agctgtctat tctgtttgtg atgcgcttcc taaaattgtc
     2881 aaaccaatca agcacttctt ttgaatattt cacagttatc tccttctatt ttgacctcca
     2941 atggaggtca tacttattac tgttatgccg gttttctttc catgtttaga atattttcat
     3001 aaaccgttat atttttaaag tcaataattt acgaatcaaa gtttccaaaa agcttttcgt
     3061 taattaaaga tcagaagtgg gttctagagt attaacatga aaccttgatc ttcaataatg
     3121 tacaataagt aaataataag agaattgcgc gataatttat ttcaggattc caattcgccg
     3181 taaacgccat cccacttttc aggggctacc agtttgattc ctgaaaagct attctctgaa
     3241 agatatgagc aaagtttgcc ttcaactata attgggcttc gaaacatttt aaggcggtaa
     3301 atactgctgt gagagacctc aacatccttc aataccagct tttccatgcg gacaatagct
     3361 gttctgcttt tatgttcaat cgctcggcac tctgatttgt ctaaatatat agcatcaaca
     3421 agccctttga tatttgccgc ataatagccg cttttgggag aatcttcttt gggtaaaaca
     3481 actgttgccg ggtaatagtc aatattatcg atctgatttt catccagtgt gttttttagc
     3541 ttttcagaaa caacaataca tccggcatcg cttaacgcaa aatatgtcat agcttttgac
     3601 ggtagggttt cgcagatgaa ttcaaggcgg gacaggtcaa catcacttga gcatattgat
     3661 gaatttcatt attaactgta cgattgacct gtctgcggca taacaagata ttgcttgtaa
     3721 agaaaattgc aactaaaatg ggtgctacga actaggacgc gctatcatga aaaattatca
     3781 aattataaac tggcaaaggt ttgacgaact tgctcttgaa tgtttcaaga aacagatcag
     3841 atcgattgaa gatcttgccg acaatgggag tcttgacctt tatgatacat ggcatcaact
     3901 ttaatatcag tcacatagat tctaaatact atacttatca acccgataaa acacacatcc
     3961 tgttatatca ttcttttcaa tagccatttt aagcctttcg ctaaaaacag ttgtctcaag
     4021 atttcttgct aaccgaaaga tgtccatatt tgcctgaatt ttatcctcac tcaacacaag
     4081 tttgtcaata aactcaacat caccatcatc gtaacaggtt attttcgatt gctcaaaatc
     4141 aatacaatca atcgcgctaa caatgttaac aattttataa tccatataag atttccgttt
     4201 atccggatca at
//
LOCUS       DAOTGZ010000231         4171 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_3064
            NODE_25287_length_4171_cov_4.430029, whole genome shotgun sequence.
ACCESSION   DAOTGZ010000231 DAOTGZ010000000
VERSION     DAOTGZ010000231.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868088
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 4171)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4171)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:22:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,785
            CDSs (total)                      :: 2,751
            Genes (coding)                    :: 2,732
            CDSs (with protein)               :: 2,732
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 12 of 19
            Pseudo Genes (internal stop)      :: 5 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4171
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_25287_length_4171_cov_4.430029"
                     /isolate="AMR_MDS_3064"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(102..1322)
                     /locus_tag="P5123_11975"
     CDS             complement(102..1322)
                     /locus_tag="P5123_11975"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRX17023.1"
                     /translation="MRKFILLLIIIYPFFLSADDDRNNYRFSQTIVVPKTENDYIEVE
                     LDQRHYPVLNKDLSDLRIIDNQEKFHPFIIQKSRRTTVKRTTIYGCDLIDSGKLKRDD
                     ILYHYNDYQITSPPNEQNLNINFINIITDNTEFSVQIEIYGRGERSSWTLLAKDTIYN
                     LQNIKKNDVLFDVTSNHLFYRVLFLAPVNNTTDFDLKAGYDLTVTDTESFNKRFVMNF
                     EIEEDQKQTVITLKNTNLLNLNSLQIKCPGLFKRRYDIRNNNRTIKRGYIYSIINNTD
                     TIEEKTISLETPDNSEELILTIYNEDDNPLSIEEIEAFYLIDKIIFKNDSDTSLTLLF
                     GNSFAEKPVYDMEHYRDTIENSNKTEGTLLEPVENADFIEVKNSGKTLFSILIIITSV
                     IFSGGILLFLLKKR"
     gene            complement(1319..3844)
                     /locus_tag="P5123_11980"
     CDS             complement(1319..3844)
                     /locus_tag="P5123_11980"
                     /inference="COORDINATES: protein motif:HMM:NF021606.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2339 domain-containing protein"
                     /protein_id="HRX17024.1"
                     /translation="MDIIERLEKLRNDHKESYTALDKALTEFERSDLFNENSDLKKEV
                     NKLSRLSSELRKELKDLHEKNSELKEALYEQITSEKISILKLSRKKLETLFSSSENKQ
                     ENLLKTLESNCKNRLNTYNKSIDEKHTEEYNKIRETVQSAVTEIDSIIFRIREKEAEE
                     LKKLRDSADKEYQQLLDTGVDEETVKKRIRQNKIEMKIGLGLINRAGIVLILLGIGAL
                     AQYSYSRFFNNYHKGSFFFLIGLLLSAGGEFTFRKKMRSFSSGLTGGGIAALYGALFY
                     SHFVLEIIGMVPALALSAVIAISSVLLTLRYNSQVIGIIALIGGFLPFFSYSFGFDLT
                     ETTKHGAMLYLLVLNTVLLAISYTKTWRAVTFISYFMNIPCLLYLVFNFENFTASVIY
                     LVIAFIVYQSVVLLKPLRHKTAPNVAEIALMVLHSFVISLSVFLLFELNDYDGYKGLI
                     ALCFTVVFIAEAVLLHFFFSKDHIFTVLIFGAALTFSVLAIPFQFGISWALFGWIIQA
                     VFLIIAGNRYNYPPVEKMGQVIGALTALFFLLYNLVPYYESGIHSSNFSFKFISLTAA
                     SILVMAYYKNLSNRQTDYKIIKTADILSFCNLGIVWLLILTLVPLHTKALAQAEIPLY
                     FYGRSELISFFVMIALTTAHLAFGFYMLIPQLFKGKAYKYLPFVSWSIAAIIIAVANF
                     TIPLGITEKNSTSLHGLAFMILIIYNIIFIAVIAYCAIKILKSLYTSLELFPLITAFW
                     ILTATVATLVVQFDLSFNSIILTGSLLILSLLFITYGFIKRYRLIRVMGLIIVISTLA
                     KFFIMDLSNLKLEYKIVSYFSYGVILIAISFIYQKLKQTVDKI"
BASE COUNT         1341 a          677 c          791 g         1362 t
ORIGIN      
        1 acttgggaaa aagcctatgg aagcatcagc ttttattaat ggagcacggc taagtaatta
       61 tgagtctttt caagaatagt ttcttaatgc tataaccatt cctatctttt tttaagcaaa
      121 aagagcaata tcccaccaga aaaaataaca gatgttataa ttataagtat cgaaaagagt
      181 gttttccctg aatttttgac ctctatgaaa tcagcatttt cgacaggttc aagaagtgtt
      241 ccctccgtct tattggagtt ttcaatcgtg tctctgtagt gctccatatc ataaacaggt
      301 ttttccgcga aactattacc aaaaagaagt gttaaagagg tatcgctatc gttcttgaaa
      361 ataattttgt ctatcagata aaaagcctca atttcttcga tgcttaaagg attgtcatct
      421 tcgttgtaaa tggtaagtat cagctcttcg ctgttatctg gggtctctaa ggaaatagtt
      481 ttttcttcga ttgtatcggt attgtttata atggagtaaa tgtaacccct cttaatggtt
      541 ctattattgt ttcgaatatc ataacgcctt ttaaacagac cggggcattt tatttgtaaa
      601 gaattaagat tcaatagatt agtatttttt aaagttatta ctgtttgttt ctgatcttct
      661 tctatttcaa aattcataac gaatctctta ttaaatgatt cggtatcagt tacggttaag
      721 tcgtagcccg ccttcaaatc aaaatccgtg gtattattta ccggtgcaag aaaaagaacc
      781 ctgtaaaaaa gatgatttga agttacatca aacaatacat catttttttt gatattctga
      841 agattgtata tagtatcctt tgcaagcagt gtccaggatg aacgttcgcc ccgtccgtaa
      901 atctcgatct gtacagaaaa ctctgtgtta tcggtgataa tattgatgaa attaatattt
      961 aaattttgtt cattgggagg tgaagttatt tgataatcgt tataatggta aagaatatca
     1021 tctcgcttta acttgccaga atcaataaga tcacagccat aaattgttgt tcttttaacg
     1081 gtagttcgtc tgcttttttg aattatgaac gggtgaaatt tctcttgatt atcgataatt
     1141 cttaaatctg aaaggtcttt atttaaaaca gggtaatgtc tttgatcaag ctctacttcg
     1201 atgtaatcat tttctgtttt gggaacaact attgtttgtg agaaacggta gttattacgg
     1261 tcatcatctg cagataaaaa aaacggatag atgataatta gcagaaggat aaattttctc
     1321 atattttgtc gaccgtctgt ttcagttttt gatatataaa ggatattgca atcaggatta
     1381 ccccatagct aaaatatgag actatcttgt attcaagttt aagatttgaa agatccatta
     1441 taaaaaattt tgcaagcgta gagattacaa taataagacc catgactcgt atcagtcggt
     1501 atcttttgat aaaaccgtag gtaataaaaa gaagagacaa aataagaagg gaacctgtta
     1561 atattatcga attaaaagag agatcaaact gaactacaag ggtagcaaca gtagctgtaa
     1621 gtatccagaa agcagttata agagggaaaa gctcaagtga tgtataaagt gattttaata
     1681 ttttgatagc gcaataggca ataacagcaa tgaatataat gttataaata atcagtatca
     1741 taaaagctag gccatgaagg cttgtgctgt ttttttcggt tattccaagc ggtattgtga
     1801 aatttgccac agctataatt attgctgcaa tagaccatga aacaaaagga agatatttgt
     1861 atgctttgcc tttgaataat tgcggtatta gcatgtaaaa gccaaaagcc aaatgtgctg
     1921 ttgtaagtgc aatcattaca aaaaaactga ttagctctga tcttccgtaa aaatagagtg
     1981 gtatttctgc ttgggcaagg gctttggtat gcaaaggaac aagggtgagt attaaaagcc
     2041 agacaattcc cagattacag aaactaagga tatctgctgt tttgattatt ttgtagtcgg
     2101 tttgtctgtt tgataagttt ttatagtagg ccattacaag tattgaagct gctgtcagag
     2161 aaataaactt aaatgagaaa ttgctggagt gtataccgct ttcgtagtag ggaaccagat
     2221 tatacaaaag aaaaaacagg gctgttaacg cgccaattac ctgacccatt ttttctactg
     2281 gagggtaatt gtaacggttt cccgcgataa tcagaaaaac tgcctgaatt atccagccga
     2341 acagtgccca gcttattcca aattgaaaag gaatggccag aactgaaaag gttaaggctg
     2401 ctccgaaaat cagaacagta aagatgtgat ccttcgagaa aaaaaagtgt aatagaacag
     2461 cttctgcaat aaagacaact gtgaaacaaa gtgcgatcag tcccttgtag ccatcgtaat
     2521 cgtttaattc gaagagtaga aaaacagata atgatataac aaaagagtgc agcaccatca
     2581 gggctatttc cgcaacatta ggggcagttt tatgccgaag cggtttaagt aaaacaactg
     2641 attgataaac tataaacgca ataacaaggt atataaccga tgcggtgaaa ttctcaaaat
     2701 taaaaacaag ataaagtaaa catgggatat tcataaaata gcttataaaa gttacggcac
     2761 gccaggtttt tgtatatgat attgcaagga gcactgtgtt aagaacaaga agatacagca
     2821 ttgctccatg ttttgtggtt tcggtaaggt cgaaaccgaa ggagtaagag aaaaaaggta
     2881 aaaagccgcc gataagtgca attattccaa tgacctgtga attatagcgt aatgtcagga
     2941 gcacagatga aatggcaatc acagccgaga gtgcaagtgc cggtaccata ccgataattt
     3001 ctaatacaaa atgtgaatag aaaagtgcgc cgtaaagtgc ggcgatacca ccgccggtaa
     3061 gaccggatga aaaggagcgc atttttttcc tgaaagtaaa ctctccgccc gcagataaca
     3121 gcagaccgat aagaaagaaa aagctgcctt tgtggtagtt gttgaaaaac cttgaataac
     3181 tgtattgcgc cagggcgccg atacccagca gaataagaac tattcccgca cgattaataa
     3241 ggccgaggcc tattttcatc tctattttat tttgacgaat acgtttttta actgtttctt
     3301 catcaactcc ggtatccagt aattgctgat actctttatc cgcggagtct ctcagttttt
     3361 tcagttcttc agcctctttt tcgcgaatcc tgaatattat gctgtctatt tctgtaacag
     3421 cagattgcac cgtttcacgg attttgttgt actcttcagt gtgtttttca tcaatgctct
     3481 tgttgtaagt atttagtctg ttcttgcagt tcgattcaag agtttttaac aggttttcct
     3541 gtttgttttc actgctgcta aacagggttt ccagtttttt tcgggataat ttaagtatac
     3601 tgattttttc gcttgtgatc tgttcgtaaa gcgcctcttt tagttcactg ttcttttcat
     3661 gaagatcttt taactctttg cgcaattctg atgataaccg ggagagcttg ttaacctctt
     3721 tttttaaatc gctattttca ttaaaaaggt cacttctttc gaactcggta agagccttgt
     3781 ccagagctgt atatgattct ttatgatcat ttctaagttt ttccagtctt tcgataatat
     3841 ccataaaaac tccagattgg gtaattgttt ttcacaggta agaactgctg aagttattgt
     3901 atataaacgg tttcgggatt gcaatgatat tttgtatgtt gctgttagct ttttcaaaag
     3961 ctaacagccc atccgttacg tgtgtattta catttgtatt gcgcttttgt cctgttaaag
     4021 atttcattat tagttagggc ttattaagtt agatatttct aaaaacaata aagattaact
     4081 cgagcaaatc ttgaatttca cgatttatag tttgataata aattattgtg aaaaaaatca
     4141 caaaaaacca attatttctt gcaaaaatta c
//
LOCUS       DAOTGZ010000232         4169 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_3064
            NODE_25310_length_4169_cov_4.038649, whole genome shotgun sequence.
ACCESSION   DAOTGZ010000232 DAOTGZ010000000
VERSION     DAOTGZ010000232.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868088
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 4169)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4169)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:22:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,785
            CDSs (total)                      :: 2,751
            Genes (coding)                    :: 2,732
            CDSs (with protein)               :: 2,732
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 12 of 19
            Pseudo Genes (internal stop)      :: 5 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4169
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_25310_length_4169_cov_4.038649"
                     /isolate="AMR_MDS_3064"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            336..1970
                     /locus_tag="P5123_11985"
     CDS             336..1970
                     /locus_tag="P5123_11985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006313833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sodium-dependent transporter"
                     /protein_id="HRX17025.1"
                     /translation="MARERWSNRGAFIMAAIGSAIGLGNVWRFPYLVGTSGGGAFFIP
                     YVIALVTTGIPLVALEYYLGIRYQHGPSEAYGFVKKRTNFIGWFALGIAAMITVYYSV
                     VISYALNYIVGSIGVKWAGREKDYFFTDILNISGGHFDFGEQVLPLVIGNAIVWIAIF
                     LIIFKGVKVVGKIVTWTVGIPWVLLAVLIVRGVMLEGAFTGLDFYLSPDFSKLADPQV
                     WLAAYGQIFFSLSLGFGLMIAYASYMPKDSDINTNAWVVSFANCATSFFSGFAIFSTI
                     GYLAAVSGVNVSDVAASGPGLAFVVYSTAIAQLPGGIILQSVFGVLFFIILFTLGIDS
                     AFSLVEGVVTGLKDTFKIDRVKATAAVCVFGFSAGLLITSRAGLYWLDSVDRWMNWGL
                     AIVGLMEAVLIGWFFNVKKASEDIDSTSSIKFGSFWIICVKYITPVILLLIIGTNFYS
                     EFNGIYEGYSKSAIMISGWGLLTFLMFIALVLQDRSSLKNPVVIIGYLVSFAGFLFAF
                     YSFYGPEIQTRFIAAAVMVVSLAIVVFCFKMAGKNQ"
     gene            2007..2096
                     /locus_tag="P5123_11990"
     CDS             2007..2096
                     /locus_tag="P5123_11990"
                     /inference="COORDINATES: protein motif:HMM:NF033493.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MetS family NSS transporter small subunit"
                     /protein_id="HRX17026.1"
                     /translation="MSLGAIVFLVLGCIIFFGGVVYGVIKMNK"
     gene            complement(2180..3529)
                     /locus_tag="P5123_11995"
     CDS             complement(2180..3529)
                     /locus_tag="P5123_11995"
                     /inference="COORDINATES: protein motif:HMM:NF015983.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HRX17027.1"
                     /translation="MMQICLINPPIEDFYYTKVRRQPLGLLYIAGTLRYAGYNVSLID
                     SHSNKKHIMSIPSSFDYLQKYFNHSDPLLRFPFHNYYHFGLSYQEIRRQIIQSKASLF
                     MISSMFTTYHEECENIINTIKEIHPFSFIAVGGYHASLYPDHFLTNTATDFVIEGEGE
                     ESALKLANAIFYNKPFDSIEGLSYKENGGISRTNRKNNLADIDSITSPARELLNLRQF
                     KFYKKNVTPLIASRGCPNNCKFCSGKIIWGNSHRYRNSDNIIKEIDECIKKYNINCFN
                     FEDENLFITKDRAVKLLEALLEYQKKNSIRLEFTAMNGISIENLDEKIISLMKKTGFN
                     EINISLVTHSEKLQLLEKRPFGTEHFFRIISYAKKMCMDVRAYFILGLPGQTKEEVIE
                     TIKYLNSLEIDFYPSVYYNVWAPYNQWKQQRSSAFFNETELLSRADLIRFFNICRKK"
     gene            complement(3562..3990)
                     /locus_tag="P5123_12000"
     CDS             complement(3562..3990)
                     /locus_tag="P5123_12000"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRX17028.1"
                     /translation="MKLKLVSIITFLCITFLLPSCEGEPLANYTIQVLCTASSQPDAT
                     FWGTYTVDGTIKNMNPEKFGTNSYVYEKDVAAEDIIEISATKDTAGSDLQIKIYKDGE
                     KINEAYLDAYKTATDEYVYTLRLEYEIPDDEEDGDNSDSE"
BASE COUNT         1167 a          643 c          847 g         1512 t
ORIGIN      
        1 aatatagtcc ggggtgtcac taacgtgcat tacgagtggg ctttttctat ataaaagatg
       61 ttttggtaca gtgggaatat tgaaaaaata ttttattccg gatatcattt ttgttatcat
      121 tttaattgat ttttacctga gaataatttg gatccatgtt aaagttatac tattattttg
      181 cacaaataaa cctaaaatgc aaataaaaag ttaaaataat tgataaataa taattatgaa
      241 ttattgaaat aattgataat tgtttgacgc gaaaaaattg ccaaccatat aatggcaaca
      301 tatttctggc aaaaacaaaa gtataggagg tttatgtggc acgagaaaga tggtcaaaca
      361 ggggggcttt tataatggct gccattggtt ctgctatagg acttggtaat gtatggcgtt
      421 tcccatatct tgtcggtaca agtggtggag gcgctttttt tataccatat gtaattgctc
      481 tggttacaac aggaatacct cttgtggcgc ttgaatacta tcttggtatt cgatatcagc
      541 atggacccag tgaagcttac ggttttgtta aaaaaagaac caatttcata ggatggtttg
      601 ctcttggtat tgctgccatg attacggtgt actattcggt ggttatctct tacgctctaa
      661 attatattgt cggttctata ggtgttaagt gggccggtcg tgaaaaggat tattttttta
      721 cagatatact taatatatcc ggcgggcatt ttgattttgg agagcaggta ttgccgctgg
      781 ttattggaaa cgctattgtc tggatcgcaa tttttctgat tatcttcaag ggagtgaagg
      841 ttgttggtaa aatagtaact tggacagttg gaatcccatg ggtactgctc gcggttctta
      901 tagtgcgagg agtgatgctt gaaggcgcgt ttacaggtct tgatttttat ctgtcacctg
      961 atttttccaa attggctgat cctcaagtgt ggcttgctgc ttatggacaa attttctttt
     1021 cgctgtcact ggggtttggt ttaatgatag cctatgcttc ttatatgcca aaggattcag
     1081 atattaatac gaatgcttgg gttgtaagtt ttgcaaactg tgcgacttca tttttttcgg
     1141 gatttgccat tttttcgaca attggctatc ttgcggctgt tagcggtgtt aatgtttctg
     1201 atgtagctgc cagtgggccg ggtcttgctt ttgttgttta ttcaacagct attgcccagc
     1261 ttcctggagg aatcatttta cagtctgttt ttggggttct tttctttata attcttttta
     1321 cgttgggtat tgactcggca ttctctttgg tagaaggtgt tgttactggt ttaaaagata
     1381 cattcaagat cgaccgcgta aaagcaactg cggcagtttg tgtgtttggt ttttctgcag
     1441 gtctgttgat tacttcacgt gccggtttat actggctaga ttctgtagac cgttggatga
     1501 actgggggct tgcgattgtt ggtcttatgg aggcagtatt gattgggtgg tttttcaatg
     1561 tcaaaaaggc ttccgaggat atagattcaa catcttcgat taaatttggt tctttctgga
     1621 taatatgtgt taaatatatt actccagtca tattgctact cattatcggt acaaattttt
     1681 acagtgagtt caatggcatt tatgaaggct attctaagtc ggccattatg ataagcggtt
     1741 ggggcttgtt aacatttctt atgtttatag ctctagtttt acaagatagg tcttctttaa
     1801 aaaaccctgt tgtcatcatc ggttatttgg tctcctttgc ggggtttttg ttcgcttttt
     1861 acagctttta tggtccagaa attcaaacaa ggtttattgc cgcggcagtt atggtggtct
     1921 ctctggcaat agttgtgttc tgcttcaaaa tggcgggaaa aaatcagtaa gtttaaatac
     1981 tataatagtt gtaattggag gccaatatga gtttaggtgc gattgttttt cttgttttag
     2041 gatgtataat attctttggc ggagttgttt acggtgttat caagatgaat aaatagtcca
     2101 atatagtctg ccttttaatg tgtaaattgg gtgctgtgat tacagtgccc aatttatttc
     2161 tgagagataa gtcttaactt tatttttttc tgcaaatatt gaaaaagcgg ataagatctg
     2221 cccttgaaag aagttcggtt tcattgaaaa aagcggacga gcgttgctgc ttccattggt
     2281 tataaggtgc ccatacatta tagtacactg aagggtaaaa atctatttca agtgagttaa
     2341 gatattttat tgtttcgatc acctcttctt tcgtttgtcc gggtaaaccc agaataaaat
     2401 aggcacgaac atccatacac atcttttttg cgtaagatat gattctaaaa aaatgttctg
     2461 ttccaaaagg tctcttctcc aaaagttgaa gtttctccga gtgggtaacc agggagatgt
     2521 tgatttcatt gaatcctgtc tttttcatga gagagattat cttttcatca aggttttcaa
     2581 ttgatattcc attcattgct gtaaattcta gtcgtatgga attttttttc tgatattcca
     2641 ataatgcttc aagaagttta accgctctat cttttgtaat aaataggttt tcgtcttcaa
     2701 agttaaaaca attaatatta tattttttta tacattcatc aatctcttta atgatattat
     2761 cactatttcg atatcgatgg ctgtttcccc aaattatctt tcctgagcaa aacttgcaat
     2821 tattagggca tcctcttgat gctatgaggg gtgtaacatt tttcttgtaa aatttgaatt
     2881 ggcgtaaatt taaaagttct cgtgcgggtg aagtaatgga atctatgtct gcaagattat
     2941 tcttgcggtt tgttcttgag atgcccccat tttctttata agataacccc tcaatactat
     3001 caaaaggttt gttataaaaa atggcgtttg ccagttttaa tgctgattct tcaccttcgc
     3061 cttcaataac aaagtctgta gctgtatttg taagaaaatg atccgggtat aatgaggcat
     3121 ggtatcctcc aacagcaata aaggagaaag ggtgtatttc cttaatagta ttaattatat
     3181 tttcacactc ttcatgataa gtggtgaaca ttgatgatat catgaaaagg gaagccttac
     3241 tctgaatgat ttgcctgcgt atttcttgat agctgagccc gaagtggtag taattgtgaa
     3301 acggaaagcg cagtaaagga tcagaatggt taaaatactt ttgaagataa tcaaaagagc
     3361 tgggaatact cataatatgt tttttgttgc tgtggctatc aataagggaa acattatagc
     3421 ccgcatagcg taaagtgcct gctatataaa gcaggccaag aggttgacgt ctgacttttg
     3481 tataatagaa atcttctatt ggaggattaa taagacatat ttgcatcatg attttttatc
     3541 aattataata acattaggca attattcaga atcgctattg tcaccatctt cttcatcatc
     3601 ggggatttcg tactctagtc taagtgtata tacatattca tcagttgctg ttttatacgc
     3661 atcaaggtac gcctcgttta tcttttcacc atccttgtat atcttgattt gtagatccga
     3721 accggctgta tcttttgtag cagaaatttc tataatgtct tcggcagcga catctttttc
     3781 atagacatat gagttggtac caaatttttc cggattcata ttcttgatcg tgccatcgac
     3841 tgtataagta ccccaaaatg tagcatctgg ttgcgaagaa gctgtacaaa gaacctgtat
     3901 tgtgtaattg gcaagaggct ccccttcaca ggaaggcaac aaaaaagtga tacaaagaaa
     3961 tgtaattata gataccagtt ttagtttcat agaacacctt aaataatgaa taaattttca
     4021 ttagaatttg gtgaattcta atgaaaatca tctcttttat aacaaaatta tagtaataac
     4081 aatcgtctgt tgctattata aaaataataa aattctatac gacttgtgtt tgaaagataa
     4141 ttaaaaaaat caggcattta taattgttt
//
LOCUS       DAOTGZ010000233         4168 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_3064
            NODE_25317_length_4168_cov_5.241430, whole genome shotgun sequence.
ACCESSION   DAOTGZ010000233 DAOTGZ010000000
VERSION     DAOTGZ010000233.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868088
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 4168)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4168)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:22:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,785
            CDSs (total)                      :: 2,751
            Genes (coding)                    :: 2,732
            CDSs (with protein)               :: 2,732
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 12 of 19
            Pseudo Genes (internal stop)      :: 5 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4168
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_25317_length_4168_cov_5.241430"
                     /isolate="AMR_MDS_3064"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1234
                     /locus_tag="P5123_12005"
     CDS             <1..1234
                     /locus_tag="P5123_12005"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRX17029.1"
                     /translation="ILGIEQKSNDPVSELIQKALKKRSMLIILDNCEHLILTAARIAE
                     CLLEAGKGIKIIATSREMLQIRGENIIPVPPLMLSDKNEKFSLPKFGQYEAVKLFIER
                     ARAVQPSFSVTSENAPDLADICQRVDGVPLAIELTAAKIQILPLDQIRERIKNPLKLL
                     NSGFRTATPRQQTLRGLIDWSYDILEPDEKILFTRLSVFSSSFDMSAAEAVCGNDCDV
                     LDVLSRLCSKSMVFREGDYRFRLLETLREYAKDKIGTEISIFEQKHFEYFSTLSAENH
                     DLYYGDLFLNKKNKMQTEYENLKQALSWSIQNCPLEGLKMARDIAPAWWSLGNNVEGA
                     RWLELLLEIQKKLSERDLTLEADANCSLGVIYHQMLKLDDSEKCSPRLCFIIKNYNLM
                     IKPKGRLLLQKEVLGLRN"
     gene            1132..2211
                     /locus_tag="P5123_12010"
     CDS             1132..2211
                     /locus_tag="P5123_12010"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRX17030.1"
                     /translation="MFTKALLYYQKLQLDDKAERKIAFAKRGLGLAQLINTFFLNTQD
                     DHSKQIRDCKANFDDSLKLYTKLDDKWGIAEALAGQAMTSAIVDNNYHDALNLMSKSL
                     QIRTDIRDYSGIGWAHFTIADYLQQLAQEENVIEHLEKAKEYFTLAGDRWGLANNGEF
                     LGFCLMIEKRYDDAEAELSQSKMLHSMIGGINQADYAKLYSVMLLIERRDDEDIEEKL
                     WKIINRAGAIGEEFIQIYGFGILLRYFQLEDQTLKVIATIDHFRTISAGMFTNQKNSF
                     IGLAITYLLLAKNNYNQAVQFIAALSKRYPQMSHEKYNIASLEYKRLTTILKEKLGNN
                     DFHDLTAIGESWSNQQFDDQLTETF"
     regulatory      2274..2380
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00162"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="SAM riboswitch class I; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="S-adenosylmethionine"
                     /db_xref="RFAM:RF00162"
     gene            2450..3745
                     /locus_tag="P5123_12015"
     CDS             2450..3745
                     /locus_tag="P5123_12015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012529450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HRX17031.1"
                     /translation="MNEKNFKQATLALHAGFDGDPATNSCAVPIYSTSSYLFDSTEHA
                     ANLFSLKEFGNIYSRLGNPTVDVFEKRVAALDGGVGALAFASGMAAITATICTLAHAG
                     ENIAASTSLYGGTVTLFNCTLAKLGIKVNFFDPDDLESLKNIINNKTRAVFIESIGNP
                     KNDIPDCKAISSIAHDAGIPLVVDNTVLTPIQFRPIDHGADIVVYSATKYLGGHGTHV
                     GGVIVDSGSFIWANDPDKWPEFCKPDPSYHGLVFTEALASIGNIAFIIHVRTHWLRDT
                     GACLSPWAAFLLLQGIETLHLRVPRHIENANIVASYLADHAKVLSVNHPSLKTHPHQS
                     RATEVLKYPGSPIVGFEVKGGIKAGSALIDNVKLARHLANIGDAKTLIIHPASTTHQQ
                     LGEEEQLKAGVSPGYIRLCVGIEDVNDIIADLEQALLAI"
     gene            3844..>4168
                     /locus_tag="P5123_12020"
     CDS             3844..>4168
                     /locus_tag="P5123_12020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007343591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRX17032.1"
                     /translation="MTRTDEQNYHIKEYHKFFTSTELLILKNGDSLSEFTLCYETHGI
                     LNGDSSNAILIFHALSGDSHIAKHHNDDHPGWWDVLVGPDKYIDTNTFFVICANTIGG
                     CSGSTG"
BASE COUNT         1296 a          899 c          850 g         1123 t
ORIGIN      
        1 aattctgggt atcgagcaga aaagcaacga tcctgtatct gaactgatcc aaaaggctct
       61 aaaaaaacgc tccatgctaa ttatcctaga taactgcgag caccttatac taacagcggc
      121 tcgcatcgca gaatgccttc tcgaagccgg taaaggcatc aaaattatag caacaagccg
      181 ggagatgctt cagatccggg gcgaaaatat catacccgta ccacctttaa tgttatcaga
      241 taaaaatgaa aaattttcac ttccaaaatt cgggcaatac gaggctgtta agctttttat
      301 agaaagagca cgcgcagtgc aaccatcgtt ttctgttaca tcagaaaacg ctcctgacct
      361 agcggatatc tgtcagagag tagacggcgt tccactggcc atagagctga ctgcagccaa
      421 aatacagata ctgccactag atcaaattcg cgaacggatc aagaatccgt taaagcttct
      481 caatagcggg tttcgcacag cgacccccag acagcaaacc ctgcgcggcc ttattgattg
      541 gagctacgat attcttgaac cggacgaaaa gattcttttt acaagactat ctgttttctc
      601 tagcagtttt gatatgagtg ccgccgaagc tgtatgcggc aatgattgtg atgtactgga
      661 cgttttatca agactctgtt caaaatcgat ggtatttcgt gaaggcgact accgatttcg
      721 ccttcttgaa acattacgcg aatacgccaa agataaaatc ggtacggaaa tcagtatatt
      781 tgaacaaaag cattttgaat acttcagcac actgtccgct gaaaatcatg acctctatta
      841 tggtgatctg tttttaaata aaaaaaacaa aatgcagacc gaatatgaaa acttaaaaca
      901 agctctgtca tggtccattc aaaactgccc tcttgagggt ttaaaaatgg ctcgcgatat
      961 tgcacctgct tggtggtctt tgggtaacaa tgtcgagggt gcacgatggc ttgaactgct
     1021 gctcgaaata caaaagaaac tttcagaaag agatctgact cttgaagccg acgccaactg
     1081 cagtctgggt gtgatttatc atcaaatgtt aaaattggac gattcggaaa agtgttcacc
     1141 aaggctctgc tttattatca aaaattacaa cttgatgata aagccgaaag gaagattgct
     1201 tttgcaaaaa gaggtcttgg gcttgcgcaa ttaataaata catttttcct gaacactcag
     1261 gatgaccact ccaaacaaat ccgtgactgc aaagcaaatt ttgacgatag cttaaagctt
     1321 tacaccaaac ttgatgataa atggggtatt gccgaagctc tggctggcca ggcaatgact
     1381 agcgctattg ttgacaacaa ctaccatgat gctctgaatc ttatgagtaa aagcctgcaa
     1441 ataagaactg atatcagaga ctactctggc ataggttggg cccactttac aattgccgac
     1501 taccttcaac agcttgctca ggaagaaaat gtaatagagc atctggaaaa agccaaagag
     1561 tactttacgc ttgcaggtga cagatggggt cttgcaaaca acggtgaatt tcttggattt
     1621 tgcctaatga ttgagaagag atacgatgat gctgaagctg agctttcgca aagcaaaatg
     1681 cttcatagca tgataggagg cataaaccag gcagattatg ccaaacttta ttcggtcatg
     1741 ttattgatag aaagaagaga cgatgaagat atcgaagaaa aattatggaa aatcattaac
     1801 agagccggcg cgattggcga agaatttatt caaatttacg gcttcggaat tcttctacgc
     1861 tattttcaac tcgaagatca gaccctaaaa gtcatagcga ctatcgacca ctttagaact
     1921 atttcggcag gaatgttcac taaccaaaag aactctttta ttgggctcgc aattacatat
     1981 ctgcttttag caaaaaacaa ctacaaccaa gctgtgcagt ttattgcagc cctgtctaaa
     2041 aggtatccgc agatgtcaca tgaaaaatac aatattgcct ccctcgaata taagcgtctt
     2101 actacaattt taaaagaaaa gcttggcaat aatgattttc atgaccttac tgccataggt
     2161 gaatcctggt ccaatcaaca gtttgatgat caattaactg aaacttttta gtataatatc
     2221 acctttttag tgattttata cttgactttt taattatttt ttttatatta acaaaataat
     2281 tgagagtgcg taagggaact ggcccgttga cgcacggcaa cctgtcacaa aaataaaatt
     2341 gataaggtgc caatgccagc ccatttgggg acgatttggt tctgtttaca gagcaaccac
     2401 ttccgtatct ccagccttaa cgtactccga aaatttcgga ggttctatta tgaacgaaaa
     2461 aaactttaag caggcaactc ttgctctgca cgcaggattc gatggtgatc ccgcgacaaa
     2521 ctcgtgcgca gtaccgattt actctacaag ctcttatctt tttgatagta ccgaacacgc
     2581 ggccaatctt ttttcactaa aagagttcgg aaatatctat tcacgattgg gaaaccccac
     2641 ggtggatgta tttgaaaaaa gagtagcggc acttgacggc ggtgtcggtg ctcttgcttt
     2701 tgcaagtgga atggcagcca ttactgcaac aatttgtact ttggcacatg ccggagagaa
     2761 cattgcggca tcaaccagcc tttacggagg cactgtaact ctttttaact gcactcttgc
     2821 taaacttgga atcaaggtaa atttttttga cccggatgat ctagagtctc tcaaaaacat
     2881 catcaacaat aagacacgag ctgtctttat cgaatcaatc ggaaatccaa aaaatgatat
     2941 tcccgactgc aaggcaataa gctcaatcgc gcatgatgcc ggcatacctc ttgttgttga
     3001 taatacagtt ttaactccta ttcaatttcg tccaatagat catggtgccg atattgttgt
     3061 ctactcggca actaagtatc tagggggaca cggcactcat gtcgggggag tcattgttga
     3121 cagcggaagc tttatctggg caaatgaccc cgacaaatgg cccgagtttt gcaaacccga
     3181 cccatcttat cacggactag tgtttacaga ggctcttgcg tctatcggca atatcgcctt
     3241 tattattcac gtgcgcacac actggcttag ggatacaggc gcttgcctaa gcccctgggc
     3301 tgcttttttg ctacttcaag gcattgaaac tcttcatcta agggttcctc gccatataga
     3361 aaacgcaaat attgttgcca gctatctagc tgatcatgcg aaagttcttt cggttaacca
     3421 tccgtcactg aaaactcatc cacatcagag ccgagcaacc gaagttttaa aatatcccgg
     3481 aagtcctatt gtagggttcg aggtcaaagg cggcattaaa gcgggatctg cgctaattga
     3541 caatgttaaa ctggcaagac atctagcaaa tattggtgac gcaaaaaccc tgatcattca
     3601 cccagcaagc actacgcatc agcaactcgg cgaagaggag cagcttaaag ccggtgtcag
     3661 tccaggttac ataagactat gcgttggcat cgaagatgtc aatgacataa tagccgacct
     3721 ggaacaggcg ctattggcaa tataaccagt tattaaaact ttacactcag tgcagttata
     3781 ttgtactgca ctgggtataa aatcagaacc gttattacta tataccatac aggacttatg
     3841 cctatgacaa gaacagacga acaaaattat catatcaaag aataccataa attctttaca
     3901 agtactgaac tacttatatt aaaaaatgga gattcactct ctgagtttac actctgttac
     3961 gaaacccatg gaattcttaa cggtgacagc agtaacgcca tactgatttt tcatgcctta
     4021 agcggcgatt cacatattgc aaagcatcac aacgatgacc accctggatg gtgggatgtc
     4081 cttgtaggac ccgacaaata tatcgacacc aatacttttt ttgttatctg tgccaacaca
     4141 attggcggct gtagtgggtc aaccggtc
//
LOCUS       DAOTGZ010000234         4163 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_3064
            NODE_25367_length_4163_cov_4.206670, whole genome shotgun sequence.
ACCESSION   DAOTGZ010000234 DAOTGZ010000000
VERSION     DAOTGZ010000234.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868088
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 4163)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4163)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:22:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,785
            CDSs (total)                      :: 2,751
            Genes (coding)                    :: 2,732
            CDSs (with protein)               :: 2,732
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 12 of 19
            Pseudo Genes (internal stop)      :: 5 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4163
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_25367_length_4163_cov_4.206670"
                     /isolate="AMR_MDS_3064"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..2279)
                     /locus_tag="P5123_12025"
     CDS             complement(<1..2279)
                     /locus_tag="P5123_12025"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRX17033.1"
                     /translation="MKKILFAALTLVVSLHCKMVAQQNYQPPVLQSPEISSLMKEVQT
                     PVSLSSGTVNINVPIYTLKHGEIEVPISLAYDASGVKVDAQPTWVGQNWSLRAGGMVS
                     RIVKGAPDETYVEEEIFLRASGQNHLLNQYPVGYAFNTPAIRNANWNTPSQIESWAKN
                     QMFELEPDEFMFNFCGHSGKFYINENGQVICTDKRYIVDMLVYINIPIYDGRVAFNIN
                     NPSPLSFTYYKSNMLPNGMGGYIDGFVQWRVSRIMGFCITAPDGTKYYFGVYHKDEDG
                     TLNIYTDYFREIEITADFFEQFFIEEFSAWYLAKIVSPKNDTVEFNYEIGDTWVNFSK
                     YYSVNKMSGHATSGVGWIFGGRVSASSFSAGDRINGKFIRPVFLSEISTPNEVIEFNR
                     SNSATLRYNYNEIHNFLYDVAQGWDRYIYIPVFEGQLPIISGYTPLGEKIIDYNYLKT
                     SKLDEIIIPSYKSFKFQYNESLSTRLQLSSINEESGTKTIPFLSFVYNTTIQIPGFLD
                     TKYDHWGYYNNKVAVVDPASSSSMSAYFAFREPDPAYQQAGILEEIRYPTGGYKKFLY
                     EPNKYNRVVKRNVGTGALSIQSSAEKTGGGLRAKEIIENDGTNSYSTHYTYLPGILNG
                     EIQYYWSNYQGKLFNGNTYTAERFFSNSLLPVSSNAEGGSVSYSKVAEYQSGNGRTEY
                     TFTNHDNTLDENSVSIDLQKSTYSPLSSKVVERGKILERLVYNEGGVTPLKRETFEYS
                     ILNNNPEYIRSVYLRRLAL"
     gene            complement(2309..2707)
                     /locus_tag="P5123_12030"
     CDS             complement(2309..2707)
                     /locus_tag="P5123_12030"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRX17034.1"
                     /translation="MSKLQFLLIVLFIMVFNNPKAVGQNIIVKDMKIQATVQTTFEEQ
                     RSVHQSFFQPVDTSKPVYIYKMGDSSNSERADTALINYLTIDKTMKIKLPDATGEDTV
                     NDFSSGPRDTADVKMKKYNHVLNKWVPGSK"
     gene            complement(2879..3280)
                     /locus_tag="P5123_12035"
     CDS             complement(2879..3280)
                     /locus_tag="P5123_12035"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRX17035.1"
                     /translation="MKNTKSSYLFIIPFLLSASILNSAVAYEISNGSKTVPVIIVPED
                     SGEIRPRNVVEAPIYCYYDTSAESVGFVFYEDIGAVTISVRNNTTGEEFFASFPSGQQ
                     NGAIQLSGTNGEYRIHITTSLGKSYSGAFTI"
     gene            complement(3516..>4163)
                     /locus_tag="P5123_12040"
     CDS             complement(3516..>4163)
                     /locus_tag="P5123_12040"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRX17036.1"
                     /translation="GVLHQSLVVAQRDFFQEKSELNAYKLKITKVYWIAAIIIIVLSV
                     SLVIYYLRNVIKANNIEAMRYMEKILEFQASKERMLEQLRELFKERFALVDELCSTYY
                     ERHTTKSEQIAIFNKVKGIIESFATDKSTKKQLEDLVNLYNENVIEKLKTQIPDTTDS
                     EIELFCFLFAGFSPCTISIFINDRIQNVYTRKSRLKKKIAKSNVPDKERFLILLS"
BASE COUNT         1188 a          868 c          772 g         1335 t
ORIGIN      
        1 aacagggcca atcgccggag ataaaccgat cgtatatatt ccggattatt atttaaaatg
       61 gaatattcga atgtttccct cttcagcggg gtaacccctc cctcgttata taccagcctt
      121 tcaagtattt ttcctctttc tactacttta ctggataaag gagaataagt ggatttttgc
      181 aggtctatgc ttacactatt ttcatctaat gtattatcat gattcgtaaa agtgtattca
      241 gtgcggccgt tcccactctg gtactccgca acttttgaat agctgacgct cccgccttca
      301 gcattacttg acacgggtaa gagcgagttt gaaaagaatc tttctgcagt gtatgtattt
      361 ccgttgaata attttccttg ataattgctc caatagtatt gtatttctcc atttagaatg
      421 ccgggcaaat aagtatagtg ggttgaataa gaatttgtcc cgtcattttc gatgatttct
      481 tttgcgcgta acccgccgcc ggttttttca gcagatgatt gtatagacaa tgctcctgtc
      541 cctacatttc ttttcacaac ccggttatac ttattaggtt catacagaaa tttcttatag
      601 ccgcccgtag ggtatcggat ttcttccagt attccggcct gctgatacgc agggtccggt
      661 tccctaaagg cgaaatacgc ggacatactt gaggacgatg cgggatcaac cacagctacc
      721 ttattgttat aataccccca gtgatcgtat tttgtatcca aaaagcctgg gatttgtatg
      781 gttgtattat atacaaatga caggaatgga atggttttgg ttccgctttc ttcattaatg
      841 gaagagagct gtaaccttgt gcttaagctt tcattgtact gaaatttaaa gcttttgtat
      901 gatggtataa taatctcatc aagtttactt gttttcaaat aattgtaatc tataatcttc
      961 tcccctaatg gagtatatcc agatataatc ggaagttgcc cttcaaatac cggaatatag
     1021 atgtatctgt cccagccttg agcgacatca taaaggaagt tgtgtatttc attataatta
     1081 taccttagcg ttgccgaatt tgaacggtta aactcaatta cttcattagg ggtgctgatc
     1141 tctgacaaaa ataccggcct gataaatttg ccgtttatcc gatccccggc tgaaaaacta
     1201 cttgctgaca ccctgccccc gaatatccat ccaactccag atgtcgcatg accggacatc
     1261 ttgtttacac tgtaatactt tgagaaatta acccatgtgt cgcctatttc gtaattgaat
     1321 tcaaccgtat catttttggg tgaaacgatc tttgccaaat accaggccga aaattcttca
     1381 atgaaaaact gctcaaaaaa atctgcggta atttcaattt ctctgaaata atccgtgtag
     1441 atattcaaag tgccgtcttc atccttatga tacacgccaa aataatactt ggtcccatcc
     1501 ggagccgtaa tgcaaaaacc cataattcgg gaaaccctcc attgaacaaa tccatcaatg
     1561 tagcctccca tgccattggg caacatgtta cttttataat aagtaaatga taaaggcgat
     1621 ggattgttta tattaaaagc cactctgcca tcatatatag ggatgtttat ataaaccaac
     1681 atatcaacaa tgtatcgctt atccgtacaa ataacctggc cattctcatt gatatagaat
     1741 tttccgctat ggccgcaaaa gttgaacata aattcatcgg gctcaagctc aaacatctga
     1801 ttctttgccc agctctctat ttgtgatgga gtattccaat tagcatttct tatggctggt
     1861 gtattaaatg cgtaacccac aggatattgg tttagcagat ggttttgccc gcttgctctt
     1921 aaaaaaatct cttcctctac gtatgtttca tcaggggctc cttttacaat gcgactaacc
     1981 attccgccag cccttagcga ccaattctgc cctacccacg tgggctgagc gtcaacttta
     2041 actccggagg cgtcataagc cagggaaatt ggaacctcta tctcgccatg ttttaacgta
     2101 tagattggca cattgatgtt caccgtcccg gaactaagtg ataccggagt ttgtacttct
     2161 ttcatcagcg atgagatttc gggactttgt aaaaccggag gctgatagtt ctgttgtgca
     2221 accatcttac aatgcaggga taccactaag gttaacgccg caaaaagtat cttcttcatg
     2281 tttctgattt ttttagatca tcagtctgtt attttgaacc gggcacccac ttgttcaaca
     2341 cgtgattgta ctttttcatc tttacatcgg cagtgtcccg gggacccgaa gaaaagtcgt
     2401 taacagtgtc ttctccggta gcatctggca atttaatctt cattgtttta tcaattgtca
     2461 gatagttgat cagtgcggta tcagctcttt cactgttgct gctgtctccc attttgtaga
     2521 tatatacggg ctttgatgtg tcaacgggct ggaagaagct ctgatgaacc gacctttgct
     2581 cttcaaaggt cgtttgtact gttgcctgga ttttcatatc cttaacaata atattctgac
     2641 caaccgcctt tggattgtta aagaccatga taaataatac gattagcaga aattgaagct
     2701 tgctcatgac tgtgagtatt tatggttaat gattctgttt gcctgtgaga ttctctcttg
     2761 tataaaaatc aactcctgac ttgatccatt ttttcatacg tacaaatatg tgcagggcat
     2821 tagagggcac tttagcaata atgacataac aaaaaggtca tcaatactat atcattattt
     2881 aaatcgtaaa agctccgctg tatgacttac ccaatgatgt ggtaatatga attctatatt
     2941 caccattcgt cccggataat tgaatagcgc cattttgttg ccctgacggg aaggatgcaa
     3001 aaaattcctc tccggttgta ttgttacgaa cactaatagt taccgcccca atatcttcat
     3061 agaagacaaa cccgacagac tcagcagaag tgtcataata acaatatatg ggtgcttcta
     3121 caacattccg gggacggatc tctcccgagt cttctggcac aataatgaca ggcaccgttt
     3181 tgcttccatt agagatttcg taagcaaccg cactattcag gatcgaagct gaaagtaaaa
     3241 aaggaataat gaacagataa gaggactttg tgttcttcat aaattaggta gtttagtttt
     3301 ggttatttaa cagttgtatc aaatttacaa gttatacaac ccgctacaaa aaaataactc
     3361 ttaaaaaatc tgtcatcccg cagcagtaaa ttaatgagta ttagacgatt aattcagtga
     3421 cagataataa aatctgtcac tgagttacat ctcacattat caatgactta catgtgcatc
     3481 ggacagatag cttcaatcca atgattttca gcgcattagg acagcaggat gagaaacctc
     3541 tctttatcgg ggacgttgga tttggcaatt tttttcttca gccgagactt acgggtataa
     3601 acgttttgta tgcgatcatt gataaaaatg ctaatcgtac agggcgaaaa tcccgcaaat
     3661 aaaaaacaaa ataattctat ttctgaatct gtagtatccg gaatctgggt tttcaacttt
     3721 tctataacat tttcattata gagatttaca aggtcttcta attgcttttt ggtactctta
     3781 tccgtggcaa agctctcgat aatgcccttg accttattga atatggcgat ttgctcgctc
     3841 ttggtggtat gcctttcgta ataggtagag caaagctcat caaccagtgc aaaacgctct
     3901 ttaaacagct ctctgagttg ttcaagcata cgctcttttg aggcttgaaa ctccaggatt
     3961 ttttccatat accgcatagc ctcaatatta tttgctttta taacatttcg caagtaatag
     4021 ataacaagtg ataccgagag cacgataata atgattgccg ctatccaata cactttagta
     4081 atttttagtt tataggcgtt tagttcggat ttctcctgga aaaagtcgcg ttgagcaact
     4141 actaatgact gatgtaaaac acc
//
LOCUS       DAOTGZ010000235         4151 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_3064
            NODE_25440_length_4151_cov_5.238525, whole genome shotgun sequence.
ACCESSION   DAOTGZ010000235 DAOTGZ010000000
VERSION     DAOTGZ010000235.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868088
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 4151)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4151)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:22:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,785
            CDSs (total)                      :: 2,751
            Genes (coding)                    :: 2,732
            CDSs (with protein)               :: 2,732
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 12 of 19
            Pseudo Genes (internal stop)      :: 5 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4151
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_25440_length_4151_cov_5.238525"
                     /isolate="AMR_MDS_3064"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(35..232)
                     /locus_tag="P5123_12045"
     CDS             complement(35..232)
                     /locus_tag="P5123_12045"
                     /inference="COORDINATES: protein motif:HMM:NF013522.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-oxalocrotonate tautomerase family protein"
                     /protein_id="HRX17037.1"
                     /translation="MPIISVELGKGQANENQKREMIERFTEDASQITGLGKEKFTILI
                     NELPKENIGVGGKMLKDLMPD"
     gene            448..669
                     /locus_tag="P5123_12050"
     CDS             448..669
                     /locus_tag="P5123_12050"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRX17038.1"
                     /translation="MRSFYKYLFYTITVLLLFGTSILAQNNDNKPENISPLPLNNEYQ
                     NRTAEHIVRYDISNSELFYTIGFIYTVIP"
     gene            complement(666..1496)
                     /locus_tag="P5123_12055"
     CDS             complement(666..1496)
                     /locus_tag="P5123_12055"
                     /inference="COORDINATES: protein motif:HMM:NF013718.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase PAP2 family protein"
                     /protein_id="HRX17039.1"
                     /translation="MNRLINCLIVVCLMLPGFINAQTAVSGNGALLKNNSVFRVDPVV
                     DGSIMLLSSMVVFVPILTESEIEYRDPESLDRNDVNSLDRGVIDNNSNGFRTASHIGV
                     VAVPLGVYGLTFLYDKNNSSSEIIQDITMISESVMVNAAFNQIMCHSFSRPRPYMYTD
                     ERDEARNDNWDYRSFYSGHTSNAMAACTAIAYIHSVRYPDSPYKTALWAASALGCTAV
                     GASRIAAGDHFYSDVFTGLIAGFSIGVLVPELHRVGRMLNPDISLNLLPAGAGVTLSF
                     "
     gene            1736..2332
                     /locus_tag="P5123_12060"
     CDS             1736..2332
                     /locus_tag="P5123_12060"
                     /inference="COORDINATES: protein motif:HMM:NF012655.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR family transcriptional regulator"
                     /protein_id="HRX17040.1"
                     /translation="MPPLRQYTKTIILNAAFEQLRKDGMESLSARSIAKQLGSSTQPV
                     YSAYKTMDDLKQELLDKAREFSITFLIQEGNTNNAFLNIGIQYLKFMHHERQLFKLLY
                     LDNSSSYGSAFTPLPAKLIEQMRKTDYLSRLDEKSLSRLLFNMQIYTHGLATMIFSGL
                     MQYDEDHVCKLLSNIGKILIKHELSETEQLSNKNHTNK"
     gene            2361..3758
                     /locus_tag="P5123_12065"
     CDS             2361..3758
                     /locus_tag="P5123_12065"
                     /inference="COORDINATES: protein motif:HMM:NF015325.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase"
                     /protein_id="HRX17041.1"
                     /translation="MKITILNGSPKGDMSLTMQYAEFIKKKFPKNKYTTFNIAQKIKK
                     IESEPAEFKKIMATIKESDAVIWAFPLYYMLVHGSYKRFIELIFERKAGAAFKNKHAV
                     TLSTSIKFFDNTAHDYIHAICDDLQMQYAGYFSASMNDLKQNETQKQLTLFTKRLFTA
                     VNNKELFARSYTPINYKQQKYSPGKAKPFIDNSSYKIIVLSGGIKPKSNLENMVNCFS
                     GQFKNDIEIIDLSKEDIKYGCLGCCHCGYDNTCILENKDGYVDLFKSRIQKADIIIVA
                     DEIHDRYLSSTWKAFLDRSFFNTHKPSLLNKQIGFLISGPYSQTGGTLQTILQSWCEF
                     HKAGNIGVATDEQSSSKITDNLIRHFAERASWGLKQQHITTDTFLHVGGFKIFRDEVY
                     GPLRTVFNADHIYYKKNGYYDFPQKKIGLRMLNAVLKILLKITPIRKRVFTQELIPGM
                     VAPLKKIVNNTKAYS"
     gene            complement(3949..>4151)
                     /locus_tag="P5123_12070"
     CDS             complement(3949..>4151)
                     /locus_tag="P5123_12070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014520445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRX17042.1"
                     /translation="LSRQTMRTIKQNLFWAFGYNVLGIPVAAGLFYLFGGPLLNPMIA
                     AAAMSFSSVSVVTNALRLKRFR"
BASE COUNT         1403 a          890 c          698 g         1160 t
ORIGIN      
        1 ccatacccga taccaaacaa attttttgtt tacttcaatc aggcataagg tccttaagca
       61 tcttaccgcc tacaccgata ttttctttgg gcaactcatt aataagtatc gtaaacttct
      121 ccttacccaa tccggttatt tggctcgcat cctctgtaaa tctttcaatc atctctcttt
      181 tttgattctc gtttgcctgg cctttaccaa gctctacact aattatcggc atatcaatcc
      241 tccactacat atattgtacc aaactacaac taatgtattt tactacacat tttgtagcaa
      301 gaattttttt agatttttct aatttttatc tttacacatg acatactggc cggattttga
      361 tacaaaatat aactctaatg ctattgtatt ttttatttac gatttgaata attcgtcacg
      421 atgatatcaa aaattaacgg ggaatatatg aggtcttttt acaaatatct attttacact
      481 attactgtac tactcctgtt cgggacttca attcttgctc agaataatga caacaagccg
      541 gaaaacatct ctcccctgcc ccttaacaac gaatatcaaa acagaaccgc cgaacatatc
      601 gtccgttacg acatttccaa cagtgaactc ttttatacaa ttggatttat atacactgtt
      661 ataccctaaa aactcagggt tactccggct ccggcaggaa gcaagttcaa gctgatatca
      721 ggattcagca tgcggcctac cctgtgcaac tccggcacaa gaacaccaat tgaaaaaccc
      781 gcgataagcc cagtaaaaac atccgagtaa aaatgatcac ccgcggctat tcgagaagcc
      841 ccaacggccg tgcatcccag tgccgatgca gcccacaagg ctgtcttgta tggactgtcc
      901 ggatatctaa ccgaatgaat atatgctatt gcagtacacg cagccatggc atttgatgta
      961 tgtcctgaat aaaaagagcg gtaatcccaa ttgtcattac gagcctcatc acgttcatca
     1021 gtatacatat atggccgcgg gcgcgaaaag gagtggcaca ttatctgatt aaaggcagca
     1081 ttaaccataa ccgattcaga aatcatggta atatcctgaa taatctcgga tgagctgtta
     1141 tttttatcgt ataaaaaagt aagcccgtac acacccaggg gaacagccac tacacctata
     1201 tgcgaggcag ttctaaaccc gttgctatta ttatctatca caccgcgatc cagagaatta
     1261 acatcatttc gatcaagaga ttccggatca cggtattcaa tttcgctctc tgtcaatatt
     1321 ggcacaaaaa ccaccattga tgataaaagc atgattgagc cgtctacaac gggatcgact
     1381 ctgaaaaccg aattattttt taataatgca ccattacctg aaacggcagt ctgtgcattg
     1441 ataaaaccag gcagcattag acatacaaca ataagacagt ttatcagtcg attcatatca
     1501 ctctccgcaa aacctgtacc tgttaaatct ctccgaccgc cataaagttt acaaaaaacc
     1561 tttacggcaa atcttaatct ttattaccag attcttacaa cctgtaactt ttttcaactt
     1621 ataaaatggg gacggtcaat cagctgaaag gtggctaaca atcccagatg aatagttgtt
     1681 gacttataac atgtgttata tatactatgt tatgtatcga tttaggagaa ctgtattgcc
     1741 accattaaga cagtacacaa aaacaatcat attaaacgca gccttcgaac aactgcggaa
     1801 agacggtatg gaaagtttat cagcacgcag tattgcaaag cagctcggtt catccactca
     1861 acccgtatac agcgcctata aaacaatgga tgatctcaag caagaactct tggacaaggc
     1921 tcgtgagttt agtattactt ttttaataca agaaggcaac acgaataatg cttttttgaa
     1981 cataggcata caatatctga agtttatgca tcatgaacga caacttttca aattactcta
     2041 ccttgataat tccagttcat atggttctgc atttactcca ttacctgcca agcttattga
     2101 acagatgaga aaaaccgact acctttctcg acttgatgaa aaatcccttt cgcgcctttt
     2161 attcaacatg cagatataca cacacggtct tgcaaccatg atattttcag ggcttatgca
     2221 atacgatgaa gatcacgttt gcaaactact gagcaatata ggaaagattc taattaagca
     2281 cgaattatca gaaaccgaac aattaagtaa caaaaatcat accaacaaat agttaaaaca
     2341 aggaatagta ggaaaaacag atgaaaatta caattttgaa cggcagccca aaaggcgata
     2401 tgagtttaac catgcaatac gctgaattta ttaaaaaaaa attcccaaaa aacaagtaca
     2461 ctacttttaa cattgctcaa aaaattaaaa aaattgagtc cgaacctgcg gaatttaaga
     2521 aaataatggc tacgattaaa gagagcgacg ctgttatctg ggcttttccg ttatattaca
     2581 tgcttgtaca cggtagctat aaacgtttta ttgagctaat ctttgaacga aaggccggtg
     2641 cggcctttaa aaacaagcac gcggtaacac tctctacctc aattaaattt tttgataaca
     2701 cagcacatga ctacatacac gcgatatgcg atgatttaca gatgcaatat gctggatatt
     2761 tttcagcatc gatgaatgac ttaaaacaaa atgaaacgca aaaacagcta acccttttta
     2821 caaaaagatt atttactgcc gttaataaca aagaactgtt tgcccgcagc tatactccta
     2881 tcaactacaa acagcaaaaa tacagtccgg gtaaagcaaa accatttatt gataatagct
     2941 cgtacaaaat cattgttctt tccggaggca ttaaacccaa aagtaatctt gaaaacatgg
     3001 taaactgttt tagcggccaa tttaaaaatg atattgaaat aatagacctt tcaaaagaag
     3061 acataaaata tggctgcctt ggttgctgtc attgcggata cgacaacact tgcatcttgg
     3121 aaaacaagga cggctacgtc gacctattca aatcaaggat acaaaaagcc gacattatca
     3181 tcgttgcgga tgaaatacac gatcgttacc tctcttcgac atggaaggca tttcttgacc
     3241 gcagtttttt taacacgcac aaaccatcac ttttgaataa gcagatcggg tttttaatat
     3301 ccggtccata cagtcaaacc ggtggcacat tacaaaccat cttgcaaagc tggtgcgaat
     3361 ttcataaagc cggcaatatc ggagttgcta ccgacgaaca gagtagctcc aaaataaccg
     3421 acaatctgat tcgccatttt gcggagagag cttcttgggg attgaaacaa cagcatatta
     3481 caaccgacac ctttttacat gtggggggat tcaaaatatt ccgtgatgag gtttatgggc
     3541 cacttagaac tgtttttaac gcggatcaca tctattacaa gaaaaatgga tattacgatt
     3601 ttccacaaaa aaagatcggc ctgcgaatgt taaacgcggt gttaaaaata cttcttaaga
     3661 ttaccccaat acgcaaacgc gtttttactc aagagcttat tcccggtatg gttgcaccat
     3721 taaaaaagat agtaaacaac acgaaagcct attcataaat gaacgtcata ttacagaaga
     3781 tatttatgct tatgtttaca atgcgagaca acaaatcaaa acttgcgttt gaacacaata
     3841 atctgagctg tacctaagat aaaaattgtg gtgaattttt taaagcagtc tttaataaat
     3901 tcaccacaat cacaattttt tacgttattc atgcctggta gagatttttt accgaaatct
     3961 ctttaaacgc agagcatttg tcacgaccga aactgaagag aacgacatcg ctgctgccgc
     4021 aatcatgggg ttaagaagag gtccgccaaa cagataaaaa agaccagcag ctactggtat
     4081 tccaagcaca ttgtatccaa aagcccagaa cagattctgt ttaattgtac gcatggtttg
     4141 gcgacttaat c
//
LOCUS       DAOTGZ010000236         4138 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_3064
            NODE_25544_length_4138_cov_7.609356, whole genome shotgun sequence.
ACCESSION   DAOTGZ010000236 DAOTGZ010000000
VERSION     DAOTGZ010000236.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868088
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 4138)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4138)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:22:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,785
            CDSs (total)                      :: 2,751
            Genes (coding)                    :: 2,732
            CDSs (with protein)               :: 2,732
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 12 of 19
            Pseudo Genes (internal stop)      :: 5 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4138
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_25544_length_4138_cov_7.609356"
                     /isolate="AMR_MDS_3064"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..384)
                     /locus_tag="P5123_12075"
     CDS             complement(<1..384)
                     /locus_tag="P5123_12075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019152201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HRX17043.1"
                     /translation="MYERAYLKQVKSRIEEPRQFIQVILGPRQVGKTTMINQLLPECS
                     IPYVYESADAITATSPVWLVQVWETARLKMKASGAAEYLLVIDEIQKIENWSEVVKQQ
                     WDKDTREHVNIKVVLLGSSRLLIQKG"
     gene            472..708
                     /locus_tag="P5123_12080"
     CDS             472..708
                     /locus_tag="P5123_12080"
                     /EC_number="2.3.1.234"
                     /inference="COORDINATES: protein motif:HMM:NF014426.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0002949 - tRNA threonylcarbamoyladenosine
                     modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     ATPase subunit type 1 TsaE"
                     /protein_id="HRX17044.1"
                     /translation="MGAGKTTFIKALYQVLWTGDQVNSPTFTLANEYLSIERYSLVSL
                     IFTASKTCRKPGRPPCTFFPPGTETNSEPRLSWC"
     gene            1124..2638
                     /locus_tag="P5123_12085"
     CDS             1124..2638
                     /locus_tag="P5123_12085"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRX17045.1"
                     /translation="MKNKTSVLLTFICLLVGTFSLFSQTSQISSSSYNRNSLSVHYVE
                     FSPALSYSKKAYDMVDVPEKYDDNTIADNVIKLNMRIPSIDGNISLTLSNAIKEQKVV
                     NKILANILLDSKGVPTVEKLAKRGLYNATDRDYLISQSAKEGISLLQQYGLETMLERI
                     YFMVIIPYSFDYGYDKNRECYGYKTAYKSFFYYIDLKNMLDSNFWELFWWQGYDQDKL
                     DYFMNYDFPIKALHVKQGTMFAEKENHSILEIQYMTLSSIINYHMIEVEQENEELQVK
                     TPVFSVSPIASKIGKKEGLKIDNLFKVTEFRQGNDGEISERKVGHVRVKHVADNRHAT
                     TGESEMSLFYKAPAGNVKKGMSLTEVPEFGTMTGFEAGLRGSFIPINDSDRFMYVGIV
                     IEQITHMWKGNRVAAGYNYLMNMETGGYCNLFTAEIKQEIQMNRISIIPGIGYGYAYQ
                     KSQSVKVSGVIASLKIAINLGKYFQINAGPKLFYFLDDSMNGLYLTAGLRLFGF"
     gene            2649..3110
                     /locus_tag="P5123_12090"
     CDS             2649..3110
                     /locus_tag="P5123_12090"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRX17046.1"
                     /translation="MKYYILICINLLIIISCGTSRQNHAIIEKRSTYIYSDADGWEVF
                     EVNSRGRNLREMSKNAKSKIVEELIRYGYKGQININPILTNPRVLKAFEKSNTDIIKK
                     IIGDDGCVQLYTRNVEGRVGSRNQSLNQATFLIKVQVNTIRNIITEYYENY"
     gene            3097..3594
                     /locus_tag="P5123_12095"
     CDS             3097..3594
                     /locus_tag="P5123_12095"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRX17047.1"
                     /translation="MKTIRIIIALAFFAGCYADSAVAQKKGNFSIGNYEVQFIRTGVE
                     GTILFKIFSYGKNYDKALENAKPDAVKAVIFKGIPGSDLMRPMVKEYEISTSQRHFFE
                     DFFSSGYYLNFVSISGDGSVDPRDVLKIKRRQYKVGVVVSVQKNNLRKYLEEADIVKA
                     LDSGF"
     gene            3611..>4138
                     /locus_tag="P5123_12100"
     CDS             3611..>4138
                     /locus_tag="P5123_12100"
                     /inference="COORDINATES: protein motif:HMM:NF040342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6175 family protein"
                     /protein_id="HRX17048.1"
                     /translation="MSLKKYFFGSKTLLLTPLLILASATLGFSQAKKPTIMVVPSVNW
                     CEQNGYTYQYNVQGRPQIMPDYDLAMAKSTELKLVIAKIAGMMGERGFPLKDLEATLQ
                     SIKNLAAEDMVTTSRSGAEIRESPLDQLRRVARADIIMELTWSVNKRGPFQSVSFILR
                     ALDAYTNKQIAESVGT"
BASE COUNT         1377 a          688 c          809 g         1264 t
ORIGIN      
        1 gcctttctga atgaggaggc gggaagaccc cagcaaaaca accttgatat ttacgtgttc
       61 acgagtgtct ttatcccact gctgcttgac gacttcgctc cagttctcta tcttttgtat
      121 ttcatcaata acaaggaggt attcggcagc tccggaggct ttcattttca gcctggctgt
      181 ctcccaaact tgcaccagcc ataccgggct ggttgctgta atcgcatccg cagattcata
      241 tacatagggt atggaacatt caggtaacaa ttggtttatc atagtggttt tacccacctg
      301 ccgaggacca agtattacct gaatgaactg tcgaggctct tcaatccttg acttaacctg
      361 ttttaaataa gcgcgttcgt acattttata caatttactc aatacggcga gtaaatttac
      421 tcaaaatatc cggaattcca aaaaatgagt gtgattgctt ttttcggacg gatgggagcg
      481 ggtaaaacta ccttcatcaa agctttatac caagtcttat ggactggaga tcaggtgaat
      541 agtcccacct ttaccttggc caacgaatac ctgtccatag aaaggtattc gttggtcagt
      601 ttgattttta ccgcatcaaa aacctgccgg aagcctggca ggccaccctg tacgtttttc
      661 cctccgggga cggaaactaa cagtgagccg agactttctt ggtgttgaat atttttgatt
      721 atcagacatg tgtgtgttta taggtgccag attaattagc aaagttactt ccttccaggt
      781 tactcatagt catcagcata ttcaagaaaa atagtgaaaa caacatccca attcaaaaaa
      841 gccacgacat tacagctgtt cacaatcagt agtcgatagt agtgtttaga ttttccatat
      901 ttatttgata gattttatca gattcaacaa gtttttgcaa tctaaacgtg taacattgtc
      961 agactcaaac cgaccgaaat caaacccatg ttaaccgaat tcaaagttgt gtaattaatc
     1021 gcctgcgata attcgtaatt ttattcaata tcggattttt ttatgtataa aagtctcatt
     1081 cttattacgc caataaataa aataatcatt aatcaactat gttatgaaaa ataaaacaag
     1141 cgttttactt acatttattt gtttacttgt aggtactttt tcattatttt ctcaaacttc
     1201 ccaaataagt tcttcaagtt ataatagaaa cagcctctcg gttcattatg ttgaattttc
     1261 gcctgcgctt tcttattcca aaaaagcata tgatatggta gatgtgcctg aaaaatatga
     1321 tgacaacact attgcggaca atgtcataaa gcttaatatg cgtattccat caattgatgg
     1381 taacatatcg ttaactctga gtaatgccat caaagaacaa aaggttgtca acaaaatctt
     1441 ggcaaatatt ctattggatt caaaaggagt gcctacagtt gaaaaattag caaaaagagg
     1501 tttatataat gctactgacc gagattattt aatatcacaa agtgcaaaag aaggaatttc
     1561 tcttctgcaa caatatggtc tggaaacaat gctggaacgt atttatttta tggttataat
     1621 accatattcg tttgattatg gttatgataa aaacagagaa tgctacggtt ataaaacagc
     1681 gtacaaatct tttttctatt atattgattt aaagaatatg cttgacagta atttttggga
     1741 actcttttgg tggcaaggat atgatcagga caagctagat tatttcatga attatgactt
     1801 cccgataaag gctttacatg tgaaacaagg gacaatgttt gctgaaaaag aaaatcattc
     1861 cattttagaa attcaatata tgaccttgtc ttcgattata aattatcata tgattgaagt
     1921 tgagcaagaa aacgaagaat tgcaagttaa aacgcctgtt ttctctgttt ctcccattgc
     1981 atcaaaaatt gggaaaaaag aaggattaaa aatcgacaat ttgtttaagg taacagaatt
     2041 cagacaaggt aacgatggcg aaatttccga aaggaaagtc ggacatgtcc gtgttaagca
     2101 tgtggcagat aatcgtcatg caacaaccgg ggaatctgag atgtctcttt tttataaagc
     2161 acctgctgga aatgtaaaaa aagggatgtc attaacagaa gttcctgaat ttggaacaat
     2221 gactggtttt gaggcgggtt taagaggaag ctttatccct ataaatgatt ctgaccgatt
     2281 tatgtatgtt ggaatagtaa tcgaacaaat aacccatatg tggaagggga atagagtcgc
     2341 agctggttat aattatctta tgaatatgga aacaggaggg tattgtaatt tgtttacagc
     2401 cgaaataaaa caagaaatcc aaatgaacag gatttctata atacccggaa taggatacgg
     2461 atacgcctat caaaaaagtc aaagtgtcaa agtttcagga gtaattgcca gtttaaaaat
     2521 agcgatcaac cttggtaagt atttccaaat aaatgcgggt ccaaaattgt tctatttttt
     2581 agatgattca atgaatggtt tatatctaac tgctggtctt cgattattcg gattctaaat
     2641 ttctagatat gaagtattat attcttattt gtataaatct tttaataata atatcatgcg
     2701 ggacatcacg acagaaccat gctattattg aaaagcgatc tacgtatatt tactcggatg
     2761 cagatggatg ggaggtgttt gaggttaatt ccaggggacg aaatcttcgg gaaatgtcca
     2821 aaaatgcaaa aagtaaaatc gttgaggaac tgattcgtta cggttataaa ggtcaaatta
     2881 atattaatcc tatcctaaca aatccacgag ttttaaaggc gttcgagaaa tcaaacacag
     2941 atataataaa aaaaataatt ggagatgatg gttgtgtaca actctacaca agaaacgttg
     3001 aaggcagagt tggctcaaga aatcaatcac tcaaccaagc cacttttctt attaaggtac
     3061 aggtgaatac gatcagaaat attataactg aatattatga aaactattag aattatcatt
     3121 gctcttgctt tttttgccgg atgctatgca gatagcgcgg tagcacaaaa aaaagggaat
     3181 ttttcaattg ggaattacga ggttcagttt ataagaaccg gtgttgaagg gaccattctg
     3241 tttaaaatat tttcctatgg caagaattac gacaaagctc tggaaaacgc aaaacctgat
     3301 gctgtcaaag ccgttatttt taaaggaata cccggatcag atttaatgag gccaatggtt
     3361 aaggagtatg agatatccac atcgcaacgt catttttttg aagatttttt cagtagcgga
     3421 tactatttaa attttgtgtc tatctcaggt gatgggagtg tagatccgcg tgatgttcta
     3481 aagattaaaa ggcgacaata caaagttgga gttgtggtgt ctgttcaaaa gaataatttg
     3541 cgtaaatacc ttgaagaggc agacattgta aaagccttag attccggttt ctaagcataa
     3601 aaaaacaatt atgagtttaa agaaatattt ttttggaagt aagacgttat tattgacgcc
     3661 tttgcttatt ctcgcgtcag cgacgttagg tttttctcag gcaaagaaac ctactataat
     3721 ggttgtgcca agcgtcaact ggtgtgaaca aaatggttat acttaccaat ataatgttca
     3781 gggtcgtcct caaatcatgc ccgattacga cttagctatg gcaaagagta ctgaactgaa
     3841 actagtcatt gctaaaatag ccggtatgat gggagagaga ggttttcctc taaaagattt
     3901 ggaagcaaca ttgcaaagta taaaaaatct tgctgccgaa gatatggtaa caaccagcag
     3961 aagtggtgct gaaataagag aatcaccgct tgatcagcta agaagagttg cgcgtgcaga
     4021 tattataatg gagttaacgt ggagtgttaa caaaagaggt ccattccaat cagtttcatt
     4081 tatattaaga gctttggatg cctacaccaa caaacaaatt gcagaaagtg ttggaaca
//
LOCUS       DAOTGZ010000237         4130 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_3064
            NODE_25612_length_4130_cov_4.746012, whole genome shotgun sequence.
ACCESSION   DAOTGZ010000237 DAOTGZ010000000
VERSION     DAOTGZ010000237.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868088
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 4130)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4130)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:22:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,785
            CDSs (total)                      :: 2,751
            Genes (coding)                    :: 2,732
            CDSs (with protein)               :: 2,732
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 12 of 19
            Pseudo Genes (internal stop)      :: 5 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4130
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_25612_length_4130_cov_4.746012"
                     /isolate="AMR_MDS_3064"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..449
                     /locus_tag="P5123_12105"
     CDS             <1..449
                     /locus_tag="P5123_12105"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRX17049.1"
                     /translation="EINGQTPEKVSRYQVNTLEYNEYRIDTSIYQNEQQLSEKIKELG
                     ARNLMAKIEFTGPNLWKTCTISDSASLFFNLETDCKYNTDIDSLIHQFRDEISIRGLF
                     YRELELRLANSSEINGIDTTFLNALLNKMNSTEELCEEDICDLYNV"
     gene            428..2533
                     /locus_tag="P5123_12110"
     CDS             428..2533
                     /locus_tag="P5123_12110"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRX17050.1"
                     /translation="MRFIQCIASHNTLFNNTIIQFKEGTNILYGESGSGKTRLVEAIN
                     HTVWNLLSSRVFSNDEINSINLNITISHNDKDFRIIRDKSGIEIQNLNDSSIASLNSS
                     HGEEEIHSIMAKFDDSLYKLLSSVNHDVYRNTSYVSSPVNEKSFFPDFKTVLPLVLSD
                     STHFFNHRIFLNDFLKNEANPFTLNYNKIKKELKEMDHEIEIKNLRFSKFSKIEQEKD
                     ELFEEYSDYEKKTEELLNKIEKLKLLESQKEKYTLLSADKKKLNESFEAEKELEENIN
                     HLLDCIKKQYPLFLSLDNAQKNSLPEIQTTFTSIQKTINLITKRKEAKAKTAQQWKKT
                     MALVTITPLIAAIFFFLNRQISLYGLEALQLALYSSITASVAAISGLTALFFSLKKYD
                     LKDLNEQKSEKDSHLVSLLEKNNIRIGKSSSTELYELILRFFQEFNSFEEMRAEISSL
                     KRSMTPNYKKYYSEKINDVSQSLNNIEDKFLIECKAIGIKCPSIDEFNYDSINADFEH
                     QIETLNEKSNGVKELLSRIDKELEQFNGSNKVGDELSSRRESVKTELNQIQKKVNTFD
                     FIESIFKTILKEKETAFFNRFSETGYVFYSNLCGKNPTLDETEFKDILMKKTDIPLSP
                     VQSHFLYLSLIITLNNFISPNSPSLPLIVDEPGLFMDKKRIDLLLPAVKKISDTRQVI
                     IITHDSNTYESFGHRINIS"
     gene            2535..3518
                     /locus_tag="P5123_12115"
     CDS             2535..3518
                     /locus_tag="P5123_12115"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRX17051.1"
                     /translation="MEENGQLHFSDDPIIPGLNEACSLIETGQLESALLILDGLLNKA
                     PDAPGIEAGYRIARFWLNRAENINDIPDGKDTADFLLEEWQIFLDYARSNNLLNTHPF
                     HAIEKYIHYTAGENYKLAFKNADNPTNNIDAILKLAICFMKLGDYNLTIDTLEYARSS
                     STANPELLALLGESYYHRRDIPKALLLFREAFFIDPTKIDLNILTAEPIIRLKEIAAE
                     EKTGWGDVKEWIPVFGHLTDIFYVKRHLTMQAVDLITKDIYTLEKTYQTMSADRVRGT
                     NTAPRLINKYLWLFDFYKEQNYNFENLSDIRSRLIEIDKKLFVPYFKNIKL"
     gene            3533..3952
                     /locus_tag="P5123_12120"
     CDS             3533..3952
                     /locus_tag="P5123_12120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013682423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="secondary thiamine-phosphate synthase enzyme
                     YjbQ"
                     /protein_id="HRX17052.1"
                     /translation="MKSFRKELFFNTSQRFELINITHDVESAITESGIKEGLCLVNAM
                     HITASVFINDDEHGLHADYFRWLEELAPHEPLSLYKHNNTGEDNGDAHLKRQIMGRSV
                     TVAITNGKLDFGPWEQIFYGEFDGRRRKRVLVKIIGE"
BASE COUNT         1472 a          786 c          718 g         1154 t
ORIGIN      
        1 ttgaaataaa cggacagacc cctgaaaagg tatcccgtta tcaggttaac acccttgaat
       61 ataacgaata ccggatagac acctcaattt accaaaacga gcagcagctt tcagaaaaga
      121 taaaagagct gggtgccagg aaccttatgg ctaaaattga gtttaccggt ccgaacctct
      181 ggaaaacctg taccattagt gattcagcct cactcttttt taatttggaa acagattgca
      241 aatacaatac agatattgac tcactaattc atcaatttag ggatgaaatc tcgataaggg
      301 gactttttta cagagagctt gaattacgat tagcaaattc ttcagagatt aatggcatag
      361 ataccacatt tttaaacgcc cttcttaaca aaatgaactc aaccgaagaa ctatgcgagg
      421 aggatatatg cgatttatac aatgtatagc ctcccacaat acactcttta acaatacaat
      481 tatacaattt aaagaaggca caaacattct ttacggagaa agtggctcag gcaaaaccag
      541 gcttgttgaa gccatcaacc atacggtttg gaacctgctt tcttctagag ttttttcaaa
      601 tgacgaaata aacagcatta acctgaatat tacgatatcg cataacgata aagactttag
      661 aataatcagg gataaaagcg gtattgaaat tcaaaatctg aatgattcct ctatagcatc
      721 gttgaactct tcgcatggtg aagaggaaat tcattcgata atggccaaat ttgatgacag
      781 cctttataag ctattgagct ccgttaacca cgatgtatac cgcaacacct cttacgtatc
      841 ttctcctgta aacgaaaaga gtttctttcc tgactttaaa acagtactgc cacttgttct
      901 ttcagacagt actcattttt ttaaccacag aatatttttg aatgattttt taaaaaacga
      961 agcaaatcca ttcactttaa attataacaa gataaaaaaa gagctaaaag agatggatca
     1021 cgaaatagag atcaaaaatc tccgtttctc caaattttcc aagattgaac aagaaaaaga
     1081 tgaactattt gaagagtatt cggattacga aaaaaaaaca gaagaacttc taaacaaaat
     1141 tgaaaaatta aaactccttg agtcacaaaa agaaaagtac accctgttat ctgctgataa
     1201 aaaaaaattg aatgaaagct ttgaagccga aaaagaattg gaagaaaata ttaaccatct
     1261 gttggattgc ataaaaaaac agtatccact ttttctctca ttagataatg cccaaaaaaa
     1321 ctcattgccc gaaatacaga caacctttac ttccattcaa aaaacaatca atctcataac
     1381 taaaaggaaa gaagcgaaag caaaaacagc acaacaatgg aaaaaaacca tggcactagt
     1441 taccataact ccactaattg ccgctatatt tttctttctt aacagacaaa tatccctata
     1501 tggattagaa gctttacaac tggctctcta ctcatccata accgcatccg ttgccgccat
     1561 aagcgggctt accgccctct ttttttcact taagaaatac gacctgaaag acctgaacga
     1621 gcagaaaagt gagaaagatt cgcatcttgt ttctttacta gaaaaaaaca acatacgtat
     1681 tggcaaaagc agcagtacgg aattatacga gcttatttta cgtttttttc aagagttcaa
     1741 cagttttgaa gagatgcgtg ctgaaatatc aagcctgaag agatccatga cccctaatta
     1801 caaaaaatat tattcagaaa aaataaatga tgtatcccaa tctcttaata atattgaaga
     1861 caaattctta attgaatgca aggccatcgg gataaaatgc ccgtcaatag atgaatttaa
     1921 ctatgattcc ataaacgccg attttgaaca tcagatagag acactaaacg agaagagcaa
     1981 tggtgtaaaa gaacttctct caagaataga taaagaactt gaacagttta acggcagcaa
     2041 caaggttggt gatgagctga gctctaggag agaatcagta aaaaccgaac taaatcaaat
     2101 tcaaaagaaa gttaatactt tcgactttat cgaatcgata ttcaaaacca ttcttaaaga
     2161 aaaagagact gcttttttta acaggttttc tgaaacagga tacgtttttt acagcaatct
     2221 ttgtggaaag aaccccactc ttgatgaaac ggaatttaaa gatattttaa tgaagaaaac
     2281 agatattccc ttgtcaccag tacaatcaca ttttttatac ctgtcgctaa taattactct
     2341 taataatttc atctctccga acagcccatc actgcctctg attgtggatg aacctgggct
     2401 ttttatggac aaaaaaagaa ttgatctttt actgcctgct gtaaagaaga tatctgacac
     2461 tcgtcaggtt attataatta cacatgattc aaacacctac gaatctttcg gtcacaggat
     2521 aaatatcagt taaaatggaa gaaaacggac aattacattt ctctgatgac ccgattattc
     2581 ccggacttaa tgaggcttgc tcgcttatag agacaggaca acttgaatcc gctttattga
     2641 tccttgacgg tcttttaaac aaggcgcctg acgctcctgg aatcgaagca ggttacagaa
     2701 tagctaggtt ttggttaaac cgggctgaaa acattaacga tatacctgac ggcaaggata
     2761 cagcagattt tctacttgaa gagtggcaga tttttcttga ttacgcacgt tctaacaacc
     2821 ttctaaacac tcaccccttt cacgcaattg aaaaatatat tcactataca gccggtgaaa
     2881 attacaaact tgcttttaaa aacgctgaca accctacaaa taatattgac gctatactca
     2941 aacttgcaat ttgcttcatg aaattgggtg attataacct gacaattgac acacttgagt
     3001 acgcccgtag ttcctcaaca gcaaatccgg agcttttggc tctgcttggc gaatcatatt
     3061 atcatcggcg tgatattccg aaagctcttc ttctttttcg tgaagctttc tttattgatc
     3121 ccactaaaat tgatctgaat attcttacag cggaaccgat aatacggctc aaagagatag
     3181 ctgccgaaga aaaaacagga tggggcgacg ttaaggagtg gattccggta tttggtcatt
     3241 tgacagatat attttacgta aaacgacatc tgacaatgca agctgttgat ttgataacaa
     3301 aagacatata tacattagaa aaaacatatc agaccatgtc agcagacaga gtcaggggta
     3361 caaataccgc accacgtctt atcaataaat acctctggtt gttcgatttt tataaagaac
     3421 aaaactataa cttcgaaaac ctttcagata taagaagcag acttatagaa attgacaaaa
     3481 aactctttgt cccctatttt aagaatataa aactgtagca gacgaggaaa atatgaaatc
     3541 attcagaaaa gaactctttt ttaatacttc acagcgattt gaacttataa atatcacaca
     3601 cgatgtagaa agtgccataa ccgaaagcgg tataaaagag gggctttgtc ttgttaacgc
     3661 gatgcatatt acagcttcgg tttttataaa tgatgatgag cacggactgc atgccgacta
     3721 ttttcgatgg ctagaagagc ttgctcctca cgaaccactg tcactttaca aacataacaa
     3781 tacgggcgag gacaacggag acgcgcatct gaaaaggcaa attatggggc gttctgttac
     3841 agttgcgatt actaacggga aactcgattt tggaccatgg gagcaaattt tttatggcga
     3901 atttgacggt agacgccgta aaagggtgct tgttaagata atcggggaat aatgcggaaa
     3961 gagcctgtta tatcattctc tccggatttg aggtatcaaa aagatagtat tcgtaagaat
     4021 tgattatgaa tgtactgttt ctttttatga tacgcttgtg caaaccgtaa ttaagatgct
     4081 catggccatg gatatgataa gcaggctcat atttgtctat aattttaaca
//
LOCUS       DAOTGZ010000238         4129 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_3064
            NODE_25618_length_4129_cov_5.345361, whole genome shotgun sequence.
ACCESSION   DAOTGZ010000238 DAOTGZ010000000
VERSION     DAOTGZ010000238.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868088
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 4129)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4129)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:22:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,785
            CDSs (total)                      :: 2,751
            Genes (coding)                    :: 2,732
            CDSs (with protein)               :: 2,732
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 12 of 19
            Pseudo Genes (internal stop)      :: 5 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4129
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_25618_length_4129_cov_5.345361"
                     /isolate="AMR_MDS_3064"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..784
                     /locus_tag="P5123_12125"
     CDS             <1..784
                     /locus_tag="P5123_12125"
                     /inference="COORDINATES: protein motif:HMM:NF018190.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_function: GO:0030246 - carbohydrate binding [Evidence
                     IEA];
                     GO_process: GO:0016052 - carbohydrate catabolic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="sugar-binding protein"
                     /protein_id="HRX17053.1"
                     /translation="KLFFQLFQKHKDIVTNVTFWGFNNGHTFLTGAPYNRPDWPFPFD
                     ENFQHTMAYKGIMQSDELPEYKPLLAVTEVKKYEAPKGTPVIDANIDAVWKKAPVVKT
                     ETIVVGDKPSIASVRVLWDKDYLYFLAEVEDSDLFLNAPEKYMNDSFEVFIDENNGKT
                     PVYEQDDFQFRVDYENLLTIGGYSDSSMIKSAAKKAGKGYIVEARFKLQTVKGATGLV
                     MGLDFQVNQNDGSGQRTAIAKWNDLTNESWRSTAGWGLVELK"
     gene            1063..1452
                     /locus_tag="P5123_12130"
     CDS             1063..1452
                     /locus_tag="P5123_12130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006065290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004635 - phosphoribosyl-AMP
                     cyclohydrolase activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosyl-AMP cyclohydrolase"
                     /protein_id="HRX17054.1"
                     /translation="MSKEYTTELLLDFGDDGEKLIPVVTQDAKSREVLILAFANKAAF
                     DETRKSGLATYWSRSRNELWKKGMTSGDTLKIEDILINCEQNSLVYLVTPQGSGACHA
                     KKEDGTAHTSCYYRRINIDNRLEFIEK"
     gene            1474..2520
                     /locus_tag="P5123_12135"
     CDS             1474..2520
                     /locus_tag="P5123_12135"
                     /inference="COORDINATES: protein motif:HMM:NF013773.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRX17055.1"
                     /translation="MDNKLTIGMPAGSLANPNRGGNLVDLLERAGFTTKGYESGGPSK
                     FTTVDFLYGWDGRPQEFGSQLAINEMDVAIAGDDWIEERRLEFELEYDTKIELKQIMS
                     LHRGGVRIVGIIDNDRPENTIDEYLLNITKEKKLLIMVSEMPYIALQWTREALKRIGK
                     LDEYNEFTVQKYKTPPAIDSGIVIYETWGKTEAKIKNGGADFGIEITQSGSALRNYGL
                     KIVDEILSSQTSIWINPALLQNIRKKELLQMFLLNLYGCINAENKVLLIFNCETSQSL
                     ALEDYLQKNNLFANEPTKASGNRYSEYTIQVAADDKNLPLAKIRYELLKLGAQNLDTV
                     PVHSSIPDISVIDI"
     gene            2869..3207
                     /locus_tag="P5123_12140"
     CDS             2869..3207
                     /locus_tag="P5123_12140"
                     /inference="COORDINATES: protein motif:HMM:NF013767.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009927 - histidine phosphotransfer kinase
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Hpt domain-containing protein"
                     /protein_id="HRX17056.1"
                     /translation="MRIDSYNMYTAADDLGIDFDKYKSLMRAFLTDNMDEYIENLRQN
                     VVANKFDEITFYAHKIAGASANYQTDEITEKAKKIKDAAKERRLIDYRAIISQMEEDI
                     KKYKKICIGV"
     gene            3207..>4129
                     /locus_tag="P5123_12145"
     CDS             3207..>4129
                     /locus_tag="P5123_12145"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HRX17057.1"
                     /translation="MMRSILVAEDEAGTGEIIKTILEDAGFNVVLRANGEEAFEAYKQ
                     QNFSAVITDLNMPGIDGEELIRLIHTINEDQIILVLSSIDDTDSIIDSMRSGIFDYIV
                     KPANKKKLLYKINRALETNELKKIKRIAEKERVVKLEHELQWYRWNEQIIARDYDRFD
                     RTLFTNLNDSFNQGSGFGTLISLLAFVFDSIKEENGSYTIDKDIYGMLKQNSEAAKQI
                     LKTFTKIATILTETLDAQIIKCTDLFDILLTLKIEMKEMSDIHKNSIHLADNNEISET
                     LMVHVNIKLITEVFRELLINAMKFSAKESKI"
BASE COUNT         1442 a          783 c          793 g         1111 t
ORIGIN      
        1 caaacttttc tttcagcttt tccaaaagca caaggatatc gttacaaacg taactttctg
       61 gggctttaac aatggacata cttttcttac tggcgcgcca tataaccgac ctgactggcc
      121 gttccctttc gatgagaact ttcagcatac aatggcatat aagggaataa tgcagtccga
      181 tgaacttcca gagtacaaac ccctgcttgc cgttaccgaa gtcaagaaat acgaggctcc
      241 caaaggtact cccgttattg acgcgaacat tgacgctgtt tggaaaaaag ccccagttgt
      301 aaaaaccgaa acaatcgttg tcggagacaa gccttcaatc gcctcggtta gagtactatg
      361 ggataaggat tatctctatt ttcttgccga agtcgaagac agcgatcttt ttcttaatgc
      421 gccagaaaaa tatatgaacg actcatttga agtatttata gacgaaaaca acggcaagac
      481 tcccgtttac gaacaagatg attttcaatt tcgtgtagat tacgaaaact tgcttactat
      541 cggcggttat tcagacagct ccatgataaa atcggcggct aaaaaagcag gcaaaggata
      601 tattgttgaa gctcgattta agcttcagac cgtaaaaggt gcgaccggat tagttatggg
      661 acttgatttt caggttaacc aaaacgacgg ttccggtcaa cgcacggcaa ttgcgaaatg
      721 gaacgatcta acaaacgaat catggcgtag cactgccgga tggggattgg tagaacttaa
      781 gtaaaccatt cagaaccgcc gtatgatacg gcggttcttt tacgatgcat aactatagct
      841 gtaaatcaca aaaaaaaagt tatcttgaca cataccctcc actttattta attttaccaa
      901 tatatttgga tacttcgaaa aatcgccatg ttataagtta tcttcgttca agagaaggct
      961 cctggtatat atcattacgc caaaacaata tggcttctta ttacctgtct aataaaaata
     1021 cattataaaa aaccgtgaca ttgccggtta ctggagaaac tgatgagtaa agaatataca
     1081 acagagttgc ttcttgattt tggagatgac ggtgaaaagc taattcctgt cgttacacag
     1141 gatgcaaaat cacgtgaagt ccttattctg gcttttgcaa acaaagctgc ctttgatgaa
     1201 acaagaaaaa gcgggcttgc aacctactgg agccgtagtc gtaacgagct ctggaaaaaa
     1261 ggaatgacct ctggcgacac ccttaaaatt gaagatattc tgataaactg cgaacaaaac
     1321 tcccttgttt accttgtgac acctcaagga agcggcgcat gccatgcaaa aaaggaagac
     1381 ggaacagcac atacatcatg ttattataga cgtataaata ttgataacag actcgaattt
     1441 atcgaaaaat agaaatttta aggacagcta acaatggata ataaacttac aataggaatg
     1501 cctgccggat cacttgccaa ccctaaccgt gggggtaacc ttgttgacct gcttgaacga
     1561 gccggtttca ccacaaaagg ctacgaatcg ggcggcccat caaagtttac aacagtcgac
     1621 tttctttacg gatgggacgg caggccacaa gaatttgggt ctcagttggc tattaatgaa
     1681 atggacgttg ccatagctgg tgacgactgg atcgaagaga ggcgcctgga gtttgaactt
     1741 gaatacgaca caaaaattga gcttaaacag ataatgtcgc ttcatcgtgg aggagtccgc
     1801 attgtcggaa taatcgacaa cgatcggccc gaaaacacga ttgatgaata tcttcttaat
     1861 attaccaaag aaaaaaaact attaataatg gtatcagaga tgccctatat cgcgcttcaa
     1921 tggacgcgag aagcacttaa gcgtattggc aagcttgacg aatataacga atttactgta
     1981 caaaagtaca agaccccacc ggcaattgat tctggcattg ttatctatga aacatggggc
     2041 aaaacagaag caaaaatcaa aaacggtgga gccgatttcg gtatcgaaat aactcaatca
     2101 ggaagcgctc ttagaaacta cggccttaaa attgtagacg aaattttaag ctctcaaacg
     2161 agtatctgga taaaccctgc ccttttacag aatatacgaa aaaaagagct gttacagatg
     2221 tttcttctta atctttatgg ttgtataaat gccgaaaaca aggtgctgct catttttaat
     2281 tgtgaaactt cccaatcttt agccttggaa gattatttgc aaaaaaacaa tctctttgcc
     2341 aatgaaccca ctaaggcttc tggcaacaga tacagtgaat acacaataca ggttgccgcg
     2401 gatgataaaa atttgccact tgcaaagatc agatacgagc ttcttaaact aggagcccaa
     2461 aatctggaca ctgttcctgt gcattcatcg atacccgata tatctgttat cgatatataa
     2521 ccaatggctc cgcaataaca gatatacctc tctctttttt cacagcttag aggcaacttt
     2581 tttgcctctt tttttgtccc ttttttactc tgaaggtaga tttttatcaa taataatgta
     2641 tcttgtatta atactgtttt atcatttaac aaaaaaaatt aataatatcg atcatagttt
     2701 taaaatgata tggctttttt tagtaaataa tgttataaca gaaatattgc gatataaaac
     2761 taaccatgat atgtgggtgt tagctgaacc aaaataaaat tacagcaaaa attaatagat
     2821 atataattaa caagattaga ccaacatatt aataattcgg agacaaaaat gagaattgac
     2881 agttacaata tgtacaccgc ggccgatgat cttggaatag attttgataa atacaagtca
     2941 ctgatgcgcg catttttaac agataatatg gatgaatata ttgaaaacct tagacaaaat
     3001 gtcgttgcta ataagtttga tgaaataaca ttctatgctc acaagattgc aggagcctct
     3061 gcaaattatc agacagatga aataacagaa aaagcaaaaa aaatcaaaga tgccgcgaaa
     3121 gagcggcgac ttatcgatta tcgcgcaata ataagccaga tggaagaaga tataaaaaaa
     3181 tataaaaaaa tctgcattgg agtttaatga tgcgttctat tcttgttgcc gaagatgaag
     3241 caggtactgg tgaaataatt aaaaccatat tggaagatgc cggctttaat gttgttctaa
     3301 gagcaaatgg cgaagaagca ttcgaagctt acaaacaaca aaatttttct gctgttatta
     3361 cagatcttaa tatgccaggc atagacggag aagagctgat acgtctgata cacaccatta
     3421 acgaggatca gattattctg gtacttagca gtattgatga cactgattcg ataatagatt
     3481 ctatgcgcag cggaattttt gactatatag taaaacccgc caataaaaag aaattacttt
     3541 ataaaattaa ccgcgcgctt gaaacgaacg aactaaaaaa aatcaagcga atagccgaaa
     3601 aagagcgcgt agtaaaactt gagcacgagc tgcaatggta caggtggaac gaacagatta
     3661 ttgcccgtga ttacgaccgt tttgaccgaa ccctttttac aaatcttaat gacagcttta
     3721 accagggatc cggttttggc acgcttatat ccctgcttgc cttcgttttt gactcaataa
     3781 aagaagaaaa cggaagttac accatagaca aagatatata cggaatgctt aagcaaaatt
     3841 cggaagcagc caaacaaata cttaaaacat ttacaaagat agccacgata ttaaccgaaa
     3901 cccttgatgc tcaaataatt aaatgtacag atctttttga catattgctt accctaaaga
     3961 tcgagatgaa agagatgtca gatattcata aaaacagcat tcatctcgca gacaacaatg
     4021 aaataagcga aacacttatg gtgcatgtga acattaaact tattaccgaa gtttttcgtg
     4081 agttgctcat taatgccatg aagttttcag caaaagagag caagatata
//
LOCUS       DAOTGZ010000239         4125 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_3064
            NODE_25651_length_4125_cov_4.276658, whole genome shotgun sequence.
ACCESSION   DAOTGZ010000239 DAOTGZ010000000
VERSION     DAOTGZ010000239.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868088
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 4125)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4125)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:22:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,785
            CDSs (total)                      :: 2,751
            Genes (coding)                    :: 2,732
            CDSs (with protein)               :: 2,732
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 12 of 19
            Pseudo Genes (internal stop)      :: 5 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4125
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_25651_length_4125_cov_4.276658"
                     /isolate="AMR_MDS_3064"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..222
                     /locus_tag="P5123_12150"
     CDS             <1..222
                     /locus_tag="P5123_12150"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRX17058.1"
                     /translation="SYLNYYRYSPHRIAALKFIKYCSHSLNRPSMAYNPSELKAVSPD
                     ALRLLSKNYFEDRMKLVKKRAEVQKALQH"
     gene            622..1431
                     /locus_tag="P5123_12155"
     CDS             622..1431
                     /locus_tag="P5123_12155"
                     /inference="COORDINATES: protein motif:HMM:NF022932.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HRX17059.1"
                     /translation="MVVNIVNKLQDLGFSANESRVYAALLEENPVTAYELGKRSGVPS
                     SKIYEVLCRLLEKEMVFELVEANRKRYIPEDPDRFINKYSGRVKQNLDELKSGFDQLE
                     LKPNVSYIISCNCRDELMQRAESLITSAGSSLLISGWAEELNQFQNHFVELAKKGVRT
                     AIVHYGKSVLSGVQCFEHPVEDILYREKGGRGFVIVADSKEAISATVFEDGSVEAAWS
                     SNSGFVSMADDYIRHDIYILKVVRHFGREMKDMFGDNYRLLRNIFADEVIK"
     gene            1428..2297
                     /gene="ilvE"
                     /locus_tag="P5123_12160"
     CDS             1428..2297
                     /gene="ilvE"
                     /locus_tag="P5123_12160"
                     /EC_number="2.6.1.42"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012636020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004084 - branched-chain-amino-acid
                     transaminase activity [Evidence IEA];
                     GO_process: GO:0009082 - branched-chain amino acid
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain-amino-acid transaminase"
                     /protein_id="HRX17060.1"
                     /translation="MKIYIDGKFYDRQDAKISVFDHGLLYGDGVFEGLRIYNGKVFLL
                     EEHLNRLFMSAKAIMLDVGISIKQMSEAVNETVRINERDKPGKSGYIRLVVTRGEGQL
                     GIDPGSCEKASVIIITEDLKLYPQKYYDEGVKLVTSSVRRNRMDSLDPRIKSLNYLNN
                     IMAKIEAKNSGAVEAVVLNDRGYVAECTADNIFIINNGVVSTPLVTDGALKGITRGFV
                     CSEVCSGLGLGVEERSMALYDLYNADECFLTGSGAEVVPAVSIDGRLIGDGKPGKFTI
                     QIAKEFSRMVNSF"
     gene            complement(2433..2822)
                     /locus_tag="P5123_12165"
     CDS             complement(2433..2822)
                     /locus_tag="P5123_12165"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRX17061.1"
                     /translation="MNKLRAFKIITLLSIPVMLTTSFVILYINPGLSKTVPDYKYLFV
                     WIALVSIIGGYLNMVYFSMIEKIGDKQKIDKLLSRRGKYYLKKDPSAFFNEEQLNEYF
                     KWQKRFVIFLVSSVLLLLILLSGKLPQ"
     gene            3114..>4125
                     /locus_tag="P5123_12170"
     CDS             3114..>4125
                     /locus_tag="P5123_12170"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRX17062.1"
                     /translation="MILFSIKLFVLFTGAMLIFVEELRRPSRINKQLLIFAVLLFLKD
                     IASYMLFNYYGRSILVTNFFFYYVAFWIPTGFFYYTVLEYFTDKLRNFRITGIIFGVM
                     LVSSAMLYQFQPLIIPYFKYSLIATRLLYAAAVIWIIVCINMAYVGAQSNFFMRHVPP
                     LNVIFTFLIVNFLTYHLPDSVFHNVVEIITYGSIAFLFFLKNQDGYDDMFKKILEAER
                     ELKSIMNLMSKSGVGSGTAFDFDEITSTILSYIADMLNARSGILLIMSADKKFLIPKS
                     FFGVFPSIDGTGNYASIKKQYLETYNRNVRVKVGEMYLGRVAQTGEAAFYQDAQKDEE
                     IRQ"
BASE COUNT         1234 a          688 c          896 g         1307 t
ORIGIN      
        1 agttacctta attattatcg ctatagtcct caccgtattg ccgcactaaa atttatcaaa
       61 tactgctctc attcgttaaa tcgcccctct atggcttata acccctccga gcttaaggct
      121 gtttctcctg acgctcttcg tttgctttca aaaaattatt tcgaagatcg tatgaagctt
      181 gttaaaaaaa gagccgaggt gcaaaaagct ttgcaacatt gatgcttttg tacttcgggg
      241 tttcttatat ataatgaata aatcgaacca aaacgcgaag aagctgacac ttttgcaaaa
      301 gatatttcaa ttgctaaaaa ggaatatctg gaatttatag aaagtaatag tttttacgaa
      361 aatcatattc ttgattttgc taatagtata aatgtttcac caggtatagt cgttggcaga
      421 ctacaacatg acggcttaat aaaatatagt tttcatgata aattaaaacg aaaagttgaa
      481 ctaaccactt aacaaccttc attggctgtt gtagcttttc attacaggga attaattatt
      541 tactgctgtt agagtcagaa acgctattta aaaaaacaaa gtgattgaca gaaagatact
      601 ctctttacta tattgactac tatggtagtc aatatagtaa acaaattaca ggatctgggg
      661 ttttcagcta atgagtcgcg agtttacgcc gcgctacttg aagaaaatcc ggtaactgcc
      721 tatgagttgg gtaagcgatc aggtgtgccc tcgtctaaga tttatgaggt tctctgtcgc
      781 ctgctggaaa aggagatggt cttcgaactt gttgaagcaa accgaaaaag gtatatccct
      841 gaagatccag atcgattcat taataaatat tcaggccggg ttaaacagaa tctggatgag
      901 cttaagtcgg gttttgatca gcttgaattg aaacctaatg tttcttacat aataagttgt
      961 aattgtcgtg atgagcttat gcagcgagcc gaatccttga tcacgtcggc cggtagctcc
     1021 cttctgattt caggttgggc agaagagctt aaccagtttc agaaccattt tgttgaactg
     1081 gcaaaaaagg gagtgagaac cgcgatagtg cactacggaa aatcggtttt atctggagtt
     1141 caatgttttg aacatccggt tgaggacata ttgtatcgtg agaagggggg ccgggggttc
     1201 gtgattgttg ccgattcgaa agaagcgata agtgcaacgg tctttgaaga tgggtcggtc
     1261 gaagcagcct ggagcagcaa tagtggtttt gtctcaatgg ctgatgatta tatacggcat
     1321 gacatctaca tactgaaagt agtacgccat ttcggcagag aaatgaagga tatgtttggc
     1381 gataattatc gcttgttaag gaatattttc gcagacgagg tgataaaatg aagatttata
     1441 tcgatggtaa attttatgac aggcaggacg caaaaatttc ggttttcgat catggcctgc
     1501 tttacgggga tggagtattt gaaggattaa gaatctacaa cggcaaggtt tttcttctgg
     1561 aagaacatct gaatcgtctg tttatgtcgg caaaggctat catgcttgat gttggaataa
     1621 gcataaaaca gatgagcgaa gctgtaaacg agacggtcag aattaatgag agggataagc
     1681 cgggaaagag cgggtatata cgtcttgtag taacgcgagg tgaagggcag cttggaatag
     1741 atcccggctc ttgcgaaaaa gcatctgtaa ttattatcac cgaggatcta aagctttatc
     1801 cgcaaaagta ctatgatgag ggagtcaaac ttgttacctc gtccgtgcgc cgtaacagga
     1861 tggattcgct tgatccgcgg attaaatctt taaactatct gaataatata atggcaaaga
     1921 ttgaagcaaa aaatagcggg gcggttgaag ctgttgtgct taacgatagg ggctatgtgg
     1981 cagagtgtac cgcggataat atttttataa taaataacgg agtggttagt actcctcttg
     2041 taacggacgg cgcgttaaaa ggtataacac gagggtttgt ctgctctgaa gtatgttctg
     2101 ggctggggct gggagttgaa gaacgttcaa tggctcttta cgatctatat aacgctgatg
     2161 aatgcttttt aaccggaagc ggagctgagg ttgttccggc agtttctatt gacggtcgtc
     2221 ttattggtga cggtaaaccg ggtaaattta caattcaaat agcaaaggag tttagccgga
     2281 tggtaaacag cttttaggaa ttcgcgatat ataacacttc tgtactcttt aatatgcttt
     2341 aaacggcata gccgttccgg aaatggtaat ctttcgaagt taccatatgt cagattcata
     2401 agaaaatgaa tctgacaatc aaatcgctgt ttttactgtg gaagcttacc cgatagaagt
     2461 attaatagca gcaaaacgga agaaacaagg aatatcacaa aacgcttctg ccatttgaag
     2521 tattcattta gctgctcttc gttaaaaaag gcagaagggt cttttttaag atagtattta
     2581 ccccttcttg agagaagctt gtcgatcttc tgtttgtcac caattttctc tatcattgaa
     2641 aaatagacca tattcaaata tccaccgatt atagatacca gtgcaatcca gacaaaaaga
     2701 tatttatagt cgggcacggt ttttgaaagt cccggattaa tatatagaat aacaaaggac
     2761 gtggttaaca ttaccggtat tgataatagg gttattatct taaaggcacg aagtttattc
     2821 ataaaatcag tctcctgaat ttttttacca ctctcacata taatagtgtt taatcaacaa
     2881 cttgttcggt tacggcggtg ttttaactaa tcgtttggta aatacaaaca cctttttact
     2941 gtattaaatt tctaattatt ggtattaatc agaaaatggt ctttataaat gtgccaatac
     3001 atgggccaaa aaaaagtagt ggaaaaatct gcggtagtgt agtatcggtt gttattgtac
     3061 tctgtttatg ggtgctatat tcgattacgt ataattttaa aagaggtagt gatatgattc
     3121 tgtttagcat taagcttttt gtgctcttta ccggagccat gcttattttt gtggaagagc
     3181 tgcgaagacc gagcagaatc aataaacagc tattaatctt tgctgtcttg ctctttttga
     3241 aggatatagc ctcctatatg ctttttaact actacggacg aagcatattg gttaccaatt
     3301 tcttctttta ttacgttgct ttctggatac ccacgggatt tttctattat acggttctag
     3361 agtattttac cgacaagctg cgtaatttca ggattacagg gattattttc ggggttatgc
     3421 ttgtctcatc ggcaatgttg tatcagtttc aaccattaat aataccctat tttaaatatt
     3481 cccttattgc cacccgtctc ttatatgccg ctgctgttat ctggatcata gtttgtataa
     3541 atatggctta tgtgggcgcg cagagtaatt tctttatgcg tcatgttcct ccactgaatg
     3601 tgatttttac atttttaatt gtgaattttt tgacatatca tctgccagat tctgtttttc
     3661 ataatgttgt agagataatt acatacggtt caattgcttt tctgtttttc cttaaaaatc
     3721 aggatggtta cgatgatatg tttaagaaaa ttctggaggc cgaacgagaa ttgaaaagta
     3781 ttatgaacct tatgagtaaa tcaggagtgg gttcaggaac agcctttgac tttgacgaga
     3841 ttacatctac catcttgtct tatatagcgg atatgctaaa cgcgcgctca ggtatacttt
     3901 tgataatgtc ggccgataaa aagtttctga ttcctaaatc atttttcgga gtattccctt
     3961 ctattgacgg aacaggtaac tacgcctcta taaagaaaca gtatcttgaa acgtataata
     4021 gaaatgttcg tgtgaaagtg ggtgagatgt atttgggacg tgttgctcag acaggtgaag
     4081 ctgcttttta tcaagatgcg caaaaagacg aagagataag acaac
//
LOCUS       DAOTGZ010000240         4061 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_3064
            NODE_26163_length_4061_cov_6.452072, whole genome shotgun sequence.
ACCESSION   DAOTGZ010000240 DAOTGZ010000000
VERSION     DAOTGZ010000240.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868088
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 4061)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4061)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:22:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,785
            CDSs (total)                      :: 2,751
            Genes (coding)                    :: 2,732
            CDSs (with protein)               :: 2,732
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 12 of 19
            Pseudo Genes (internal stop)      :: 5 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4061
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_26163_length_4061_cov_6.452072"
                     /isolate="AMR_MDS_3064"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(153..1307)
                     /locus_tag="P5123_12175"
     CDS             complement(153..1307)
                     /locus_tag="P5123_12175"
                     /inference="COORDINATES: protein motif:HMM:NF012678.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-containing alcohol dehydrogenase"
                     /protein_id="HRX17063.1"
                     /translation="MIYPDFHLPTRLYLQPDIIKNVGEIIASRNAHRALIITSSDDYE
                     KYESIIEFIVENLNANGVISIIYDEIPGKANNEYIDSAVYYAKKTNTDIIIGVGLIES
                     LNSAKAIAMLANNYYFCDDIFEYPKVNDPIPYALIPFMPSIGLEIVPCFYMPDIHRNL
                     KRIYYNNKLYPDLTIIDPLLSLQAEDSDFANASVAALSIAVESLISQTTSDITNTYAL
                     KAVDLTYRNLAAAFRDPDNTSYRAPLALASVLSGISLTTSFMSITFAISLALSSYTPI
                     PLSKSMGILLPHIMEYNLTTSTNKYVQIARIMDEDVHDITIIEAAIKAIEAVRKITLD
                     VEIPQRLSEFEISKTLLSKVSELAQTYPFIKNAPRTLNKDELETILVAAF"
     gene            complement(1419..1718)
                     /locus_tag="P5123_12180"
     CDS             complement(1419..1718)
                     /locus_tag="P5123_12180"
                     /inference="COORDINATES: protein motif:HMM:NF016223.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthesis anti-sigma factor FlgM"
                     /protein_id="HRX17064.1"
                     /translation="MVLNKIGNINKIFETKKSNSVNKANTTGSSSDSIQLSTEGLKAA
                     EEAKTLQMVRETPDVRREKVEAIKKQMQDGTYDRHLDDKVLGLVAGKLLNGIFSE"
     gene            complement(1825..2508)
                     /gene="rsmI"
                     /locus_tag="P5123_12185"
     CDS             complement(1825..2508)
                     /gene="rsmI"
                     /locus_tag="P5123_12185"
                     /EC_number="2.1.1.198"
                     /inference="COORDINATES: protein motif:HMM:TIGR00096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008649 - rRNA methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0000451 - rRNA 2'-O-methylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (cytidine(1402)-2'-O)-methyltransferase"
                     /protein_id="HRX17065.1"
                     /translation="MENSPRLYVIATPIGNLKDITLRALEVLKEEVSILFCEDTRVSA
                     RLLSHYGVHLQLISLHAHSTNKPIENAIKALKEGKTVAYVSDCGTPGISDPGSKLVEA
                     VSEAGFTVTPLPGASAVTSLISVSGFSGKQFIFAGFISKKPGRRINELTELKKFDGTI
                     VIYESPHRIKKTLEAICEVFPDTSITIGREITKKFEEIIRTNSANMRFLLDNLTEKGE
                     FSIAIHNKL"
     gene            2778..2972
                     /locus_tag="P5123_12190"
     CDS             2778..2972
                     /locus_tag="P5123_12190"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRX17066.1"
                     /translation="MTSYEKTSYQLAVFALARESKYSRGGDLIRSKIYKKSETVAEAA
                     VFLYLDCRFGGYPENVRRIL"
     gene            2969..>4061
                     /locus_tag="P5123_12195"
     CDS             2969..>4061
                     /locus_tag="P5123_12195"
                     /EC_number="2.3.3.13"
                     /inference="COORDINATES: protein motif:HMM:NF002991.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-isopropylmalate synthase"
                     /protein_id="HRX17067.1"
                     /translation="MKARYAAMSKIDNENRIWPSAKITVSPEWCAVDLRDGNQALPNP
                     MTPEQKMEYFKLLLDIGFKQIEIGFPSASEDDFQFCRDIIEQNLIPNDVTISVLVQSR
                     KHLIERTIEALRGAPRAVVHQYIATSELHMKHVFGMTREDVMKTAVEGCSLIKKGVQQ
                     LREEGSVVGLEFSPEEFSDTDIDFALDVCHAVVEEWNPEEGEKVILNLPMTVERRLPN
                     EYADMIEVFMRRNKYPKKTVVSVHVHNDMGCAVAASTLALMAGAQRVEGTLFGHGERT
                     GNLDLVTIALNLEYLGVNTGLDFSKLDRITSIVERVTDMPVHARQPYSGQLVFTAFSG
                     SHQDAINKGFSKKSELIDTFGGWKIPYLHV"
BASE COUNT         1187 a          684 c          874 g         1316 t
ORIGIN      
        1 caccaccacc aatagttcgt ggcatttcgg gcaagtatat tgttggaatt gccaaagttg
       61 attctggatt tgttgtaata ctggatattg accagatttt ctcacacgaa gagctcagtt
      121 ctataccggg taaataaatg tatggaagcg gtttaaaacg ccgctaccag tattgtttcc
      181 aactcgtctt tgtttagtgt tcttggtgcg ttcttgataa atggataggt ttgagcaagt
      241 tcgctgactt tacttaaaag tgtttttgat atttcaaatt cactcaatct ttgtggtatt
      301 tctacatcca atgtgatttt tctgacagcc tcaatggctt taatagcagc ttcaataatt
      361 gtgatatcat gaacatcctc gtccataatg cgcgcaatct gtacgtattt gttagtggat
      421 gttgtcaggt tatattccat aatatgtggt aacagtatgc ccatgctttt ggacagcggt
      481 ataggcgtat aagaagagag tgcaagtgat atcgcaaagg ttatggacat aaatgatgtt
      541 gttaatgata ttccgctaag tacagacgca agtgcaagtg gcgctcgata gcttgtatta
      601 tcgggatctc tgaaagctgc tgcaagattt cggtaggtga ggtctacggc ttttagggca
      661 tatgtgtttg ttatatcgct tgttgtttgc gatataagtg attcaactgc gattgatagc
      721 gcggcaacgc ttgcattagc gaaatccgaa tcttcggcct gaagcgaaag aagtgggtca
      781 attattgtca gatcaggata aagtttattg ttgtagtaaa ttcttttcag atttcgatga
      841 atatcgggca tgtaaaaaca gggtacaatc tcaaggccta ttgagggcat aaagggaata
      901 agagcataag ggattggatc gtttacctta ggatactcga aaatatcatc acaaaaatag
      961 tagttgtttg caagcatggc gatagctttg gcgctgttca ggctttcaat caggccaaca
     1021 ccaataataa tatctgtatt tgtctttttt gcgtaatata ctgcggagtc gatatattcg
     1081 ttatttgctt tacctgggat ttcatcataa ataatgctga taacaccatt tgcgtttagg
     1141 ttttctacga taaactcaat aatgctctcg tatttttcgt agtcatcgga tgaagtaata
     1201 ataagagcac ggtgggcatt tcttgacgct attatttcac cgacattctt tataatatcg
     1261 ggctgaagat acaatctggt cggaagatga aaatcaggat atatcatatg cgcgctgttc
     1321 ctgttaaatg tttaaggtga ttttttcata ttcttttaca aaaaaaagcg gcatagatat
     1381 accgcttttg tatcataaac cgtcacatac aaacggtgtt attcgctgaa aataccgttt
     1441 agcaacttgc cggccacaag accaagaacc ttgtcatcaa gatggcggtc gtatgtgccg
     1501 tcctgcatct gtttttttat cgcttctacc ttttcgcggc gcacatcagg tgtttctctg
     1561 accatttgaa gagttttggc ctcttccgca gctttaaggc cttcggtcga aagttgaata
     1621 gagtcagacg aagaaccggt tgtgttggct ttattgaccg aatttgattt ttttgtttcg
     1681 aaaatcttgt ttatattgcc aattttattg agtaccattt atataaccct tgtttgatct
     1741 aatactatta tcggtaaagt aagacaaatc tttaactatt aaggtaattt tgtgtaaaat
     1801 aaaataaaac tttatgacac aatatcatag tttgttatga attgcaatag aaaattcacc
     1861 tttttctgtt aaattgtcta ataaaaaacg catattggcg ctgtttgttc tgattatctc
     1921 ttcaaatttt ttagtaatct ctcttccgat tgttatggaa gtatccggaa aaacttcaca
     1981 aattgcctct aatgtctttt ttatgcgatg tggtgattcg taaattacga ttgtaccgtc
     2041 gaatttttta agttcagtga gttcgtttat tcgtcgccca ggttttttgc taataaaacc
     2101 tgcaaaaata aactgtttcc cgctaaaacc agatacagat ataagcgatg ttacagcaga
     2161 tgctccagga agcggtgtta cagtgaatcc cgcttcagaa acagcttcta ctagtttact
     2221 tccgggatca gatatacctg gagttccaca atccgaaaca tatgcaactg ttttgccttc
     2281 tttaagtgct ttaatggcgt tttcgatagg tttatttgtt gaatgagcat gaagagatat
     2341 caattggaga tgtactccat agtgggatag tagtcgggca gatacacggg tatcttcgca
     2401 aaaaagaata cttacttctt ctttaagtac ttcgagggcg cggagagtta tatctttcag
     2461 attacctata ggagtcgcaa taacgtaaag ccgggggcta ttttccatta atattctcca
     2521 gattattgag tgttaaaaac tatatttgat atggatagaa tacaaaggtc actataatca
     2581 tggtatgatg tatatcgtca attacataaa actatgatat tcccacttgt tagaattaag
     2641 ttaatggtta cagttcgcgg ataagagggg atgaacatag tgctaaagaa aagtaaatga
     2701 ataaaatagt acaaaatcta gcctgaaaac aataagagta tttaattatt attgacattt
     2761 atgtgcaaaa ttagtatatg acaagttatg aaaaaacaag ttatcagctc gctgtctttg
     2821 ctcttgctcg agaatctaaa tattcccgcg gtggtgatct gatacgctca aaaatttata
     2881 aaaaatcgga aaccgtcgcg gaagcggcgg tttttttata tttagactgc cgctttggcg
     2941 gttatccgga aaatgtaagg agaatactat gaaagcccgt tatgcagcaa tgagtaaaat
     3001 tgataatgaa aaccggatat ggccttcagc caaaatcacg gtgtcacctg aatggtgcgc
     3061 tgttgatctt cgtgatggaa atcaggcctt gcctaatcca atgacaccgg aacaaaaaat
     3121 ggagtatttc aagttgctgt tagatatcgg gtttaaacag atcgaaattg gttttccttc
     3181 cgcttctgaa gatgattttc aattttgccg tgatataatt gagcagaatc ttattcctaa
     3241 tgatgttact atttccgtgc ttgttcagtc acgcaagcat ctgatagagc ggacaattga
     3301 agcgcttaga ggtgcgccta gagcggttgt tcatcagtat atagctacaa gtgagctgca
     3361 tatgaaacat gtatttggga tgacacgtga agatgtgatg aagactgcag tagaaggctg
     3421 ttcacttata aaaaaagggg ttcaacagtt aagagaagag ggaagtgttg ttgggcttga
     3481 gttttctccg gaagaatttt ctgatacaga tatagatttc gctttggatg tgtgccatgc
     3541 cgttgtcgaa gagtggaatc cggaagaggg tgaaaaagta atcttgaatt tgccaatgac
     3601 ggtcgaacgg cgacttccaa atgaatatgc agatatgatt gaagtattta tgcgtcgtaa
     3661 caaatatccg aaaaaaactg tggtttccgt tcatgtacat aatgatatgg gctgtgcagt
     3721 agctgcttct acattggcac ttatggctgg agctcaaagg gtcgaaggta ctctttttgg
     3781 acatggtgaa agaacaggta atcttgatct tgttacgatt gctttaaacc tggaatatct
     3841 tggtgtgaat acaggccttg atttttcaaa actggatcgg attacatcca tcgttgaaag
     3901 agtaacggat atgcccgtgc atgcacgaca accgtattcc ggccagttgg tgtttacggc
     3961 tttttcaggt tctcatcagg atgctattaa caaaggattc agcaaaaaaa gtgaattaat
     4021 tgatactttt ggcggatgga agatccctta tcttcatgtc g
//
LOCUS       DAOTGZ010000241         4049 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_3064
            NODE_26267_length_4049_cov_4.553580, whole genome shotgun sequence.
ACCESSION   DAOTGZ010000241 DAOTGZ010000000
VERSION     DAOTGZ010000241.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868088
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 4049)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4049)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:22:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,785
            CDSs (total)                      :: 2,751
            Genes (coding)                    :: 2,732
            CDSs (with protein)               :: 2,732
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 12 of 19
            Pseudo Genes (internal stop)      :: 5 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4049
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_26267_length_4049_cov_4.553580"
                     /isolate="AMR_MDS_3064"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..633)
                     /locus_tag="P5123_12200"
     CDS             complement(<1..633)
                     /locus_tag="P5123_12200"
                     /inference="COORDINATES: protein motif:HMM:NF024886.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HRX17068.1"
                     /translation="MFDLIDRFAEYMVNERGASDNTITAYTSDLIQMVDFFLNADKDV
                     YPLDCLVTGEDINIESITTTDLKCYIGFLSDRECDSSTIERKISSTKRFFSYLFNMSI
                     IDCNPARILIYPKKKLRLPSFLNDRHIDKLFNFELKNFIDYRDMALLHIIYSTGCRVS
                     EIAGASVNDLDIASNRLKVHGKGGHDRFTFIGKEALKYYMLYCQKRKDKGF"
     gene            complement(626..2731)
                     /gene="topA"
                     /locus_tag="P5123_12205"
     CDS             complement(626..2731)
                     /gene="topA"
                     /locus_tag="P5123_12205"
                     /EC_number="5.6.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013244953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003917 - DNA topoisomerase type I (single
                     strand cut, ATP-independent) activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I DNA topoisomerase"
                     /protein_id="HRX17069.1"
                     /translation="MDKKTLVIVESPTKVKTITKYLGKDYIISSSRGHIIDLPKSRMA
                     VDVDHNFEPEYITIRGKAKVLNELKKFAKKADTVLLATDPDREGEAISWHLANSLAKF
                     CPNIKRIEFNEITKNAVLDAVKNPRELNMSLVNAQQARRILDRIVGYSISPILWKKVK
                     KGLSAGRVQSVALKVICEREEQIDAFNPEEYWTYDIAFKIKGREYSASLYAFNNEKIS
                     ISTREEIDDITSILENSTFVIESLKSKKRTRKPIAPFITSKLQQDAANRLGFTTSKTM
                     MVAQQLYEGIDLGGEDGQVGLITYMRTDSTRISQGAIESVRKYISENYSDDYLPEEPN
                     SYSNKKGSQDAHEAIRPTEVFRTPESIKKMLSGEQYKLYNLIWKRFVSSQMTPERSEI
                     YTLVINGSGYLFRSSATTVLFDGFAVLDKEGKAKKAVFPKVDEGDEVNVSGHMPEQHF
                     TSPPPRYNDASLVKFLEESGIGRPSTYAPTINTLIKRYYVNRSGRQLVPTVLGKLVNT
                     LVKDNFANLVSIEFTSGMEEKLDSIELEKTEWHQMLREFYDPFIQGVHDAEENIDNLK
                     GVLDEETDLVCELCGAPMLKKLGKFGYFLACSAFPDCRNAKSLPLGRCPQCEGDVVKR
                     SSKKGRPFYACSEYPECEFVTRDLPADKDCPKCGKLLFKKRIRGKGERLVCLNEECGY
                     FMDLLDEVEEASKKEDYSV"
     gene            complement(2962..>4049)
                     /gene="dprA"
                     /locus_tag="P5123_12210"
     CDS             complement(2962..>4049)
                     /gene="dprA"
                     /locus_tag="P5123_12210"
                     /inference="COORDINATES: protein motif:HMM:TIGR00732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009294 - DNA-mediated transformation
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="DNA-processing protein DprA"
                     /protein_id="HRX17070.1"
                     /translation="AIFEDVRPEHRDCVAHIKKIYGADVFKAADKILNRCAKIGVDII
                     PMTSSQYPLLLSRIADPPLVLYRQGEFCAKPAIAIVGSRNSNNKSEDVTARLCCGIAG
                     YNHALVSGIARGIDRSVHVAALQYQAPTIAVTACGLDKYYPGENRDLFKKIRETPQCS
                     LISEHPPGVGVMKWTFVRRNRIISGLCKATVVVQAGINSGALITARCAIEQNRELFVT
                     PCYPYDSSYYGSLNLINQGAIALMSHEQIFSILGDTISNIEESQILPKNEPAERILQS
                     NLNFDTVNGKEDDVTADLLLKIDMDKLSKDGKSLAQFICDGVSDCDELVRLTGISSAR
                     VSEILLEFELENYIKRTANHIMPLTEK"
BASE COUNT         1179 a          899 c          695 g         1276 t
ORIGIN      
        1 aaatccctta tcttttcgtt tttgacaata taacatatag tacttaagag cctctttgcc
       61 gataaaggtg aaacggtcat gcccaccctt tccgtgaacc ttgagccgat tggaagctat
      121 atccaggtca ttaaccgatg cgcctgcaat ctcggataca cggcatccgg ttgaataaat
      181 aatatgcagc aaagccatgt cccggtaatc aataaaattt ttaagttcaa aattaaaaag
      241 tttatcaata tgccgatcat tcagaaacga tggaagcctt aacttttttt taggataaat
      301 aagtatccta gccggattac aatcaattat tgacatatta aacaggtaag agaaaaacct
      361 ctttgtactc gaaattttgc gttcaattgt cgaactgtca cactcacggt cagataaaaa
      421 gccaatataa catttcaaat ctgttgtggt tatagactcg atattaatat cttcccctgt
      481 taccaagcaa tccaaaggat aaacatcctt atcagcattc aaaaaaaaat caaccatctg
      541 tataagatca cttgtatagg cagttatagt attatccgaa gccccccttt cgttaaccat
      601 atactccgca aatctatcga tcaaatcaaa cactataatc ttccttctta gaagcctcct
      661 caacttcatc aagaaggtcc ataaagtatc cacactcttc gtttagacat acaagacgtt
      721 cacccttgcc tctaattctt ttcttaaaaa gaagttttcc acattttggg caatctttat
      781 cagcaggtaa atctctcgtt acaaattcac attctggata ttcactacaa gcatagaatg
      841 gacgtccttt tttagatgac ctctttacta catcgccttc acactgcgga catcttccaa
      901 gcggcaaaga tttggcatta cgacaatcag gaaacgcact gcaagccaga aaatatccga
      961 atttaccaag tttctttaac atgggtgccc cacaaagctc acatacaaga tcagtctctt
     1021 cgtcaagcac tcccttaaga ttatcaatat tctcttcggc atcgtgaaca ccctgaataa
     1081 acggatcata aaattcacgt agcatctgat gccattcggt tttttcaagt tcaatgctat
     1141 ccaatttctc ttccattccc gaagtaaatt caatagaaac aagattcgca aaattatctt
     1201 taacaagagt attaaccagt ttaccaagaa cagttggaac aagctgcctt cctgaccgat
     1261 taacataata tcgtttaatc agggtattga tcgttggcgc atatgtggac ggtctgccaa
     1321 tacctgattc ctccagaaac ttcactaaag atgcgtcatt ataacgtggt ggaggtgatg
     1381 tgaagtgctg ttccggcata tgcccagaaa catttacctc gtccccttcg tcgacctttg
     1441 ggaaaacagc tttttttgcc ttgccttctt tatcaagcac tgcaaaaccg tcaaaaagaa
     1501 cggtagttgc ggatgatctg aatagatatc ctgatccgtt tattacaaga gtatatattt
     1561 cagatcgctc gggagtcatt tgagaagata caaatctttt ccagataaga ttatataatt
     1621 tgtactgttc accagaaagc atcttcttaa ttgattccgg tgttctgaaa acctctgtcg
     1681 ggcgtattgc ttcgtgagca tcttgtgatc cctttttgtt tgagtaactg ttaggttctt
     1741 caggaagata atcatcggaa tagttttctg agatatattt cctaacagat tctatagccc
     1801 cttgagagat tctggtcgag tcggtacgca tatatgtgat aagccctacc tgtccatcct
     1861 ctccgccgag gtctattccc tcataaagct gttgggcgac catcatcgtt tttgaagtag
     1921 taaagccaag cctattagcg gcgtcttgtt gaagttttga agtaataaac ggtgctatcg
     1981 gtttacgagt cctttttttg gatttcaacg actctatcac aaatgtgcta ttttcaagta
     2041 ttgaggttat atcatcaatc tcttctcggg tcgaaataga aattttctcg ttattgaacg
     2101 cataaagaga agccgagtac tcacgcccct ttattttaaa agctatgtca taagtccagt
     2161 actcttcggg attaaatgca tcgatctgtt cttccctttc gcaaataacc tttaacgcta
     2221 ccgactgcac tcgtcctgcc gacagcccct tcttgacttt cttccataat attgggctta
     2281 tcgaataccc cacaatgcga tccagaattc ttctggcctg ttgggcatta acaaggctca
     2341 tgtttaattc gcgcggattt ttgacagcat caagaacagc attcttggta atttcattaa
     2401 attcgattcg ctttatgttt ggacaaaact ttgcaagaga attggcaaga tgccaggaaa
     2461 ttgcttctcc ttcacggtca gggtccgtcg cgagtaaaac tgtatcagct tttttcgcaa
     2521 actttttaag ttcatttaag accttggcct ttccacgaat agtaatatat tcaggttcga
     2581 aattatgatc aacatcaaca gccatacgtg actttggaag atcaataata tgccctcgcg
     2641 atgaagatat tatatagtct ttaccaagat actttgttat tgtttttacc tttgtaggtg
     2701 actccactat gaccaacgtc tttttatcca tattcgatcc tgtgagtttt tattaagtgg
     2761 tttttcattt tttatcagat catcgaaatg ctttgttgcc ttaaaccagt tattttcagc
     2821 ataaacagtt cgacagtcta acattttttt gacaataata atcattttaa tagaagaaac
     2881 caagacaatt agactaaggg aacaaagagt atggatttgt caaacaattt gcgggttaca
     2941 acaaaaagtt ttaattgcgt gttatttttc ggttaatggc ataatatggt tagccgttct
     3001 ttttatataa ttttctagct caaactcaag aagaatttca ctcactcgag ccgaagagat
     3061 ccccgttaat cttacaagtt catcacagtc gctcaccccg tcacagataa actgcgcaag
     3121 actctttccg tccttggaaa gcttatccat atcaatcttc aataacaggt cggcagttac
     3181 atcatcttct tttccgttaa ccgtatcaaa attcaaattg ctctgtaaaa ttctttctgc
     3241 aggttcattt ttcggtaaaa tttgagattc ttcaatatta cttatagtgt cacctaaaat
     3301 gctaaaaatc tgttcgtgac tcattaaagc tatcgcgccc tgatttataa ggttaagcga
     3361 tccataatag gagctatcat acggataaca gggagtcaca aatagttcac ggttctgctc
     3421 tatcgcgcat ctcgccgtta tcaacgcacc actatttata cctgcctgta ctactacagt
     3481 tgctttgcat aaaccactta tgattctatt acgtcgcaca aaggtccatt tcatcacacc
     3541 aacacctgga ggatgttctg aaataagcga acattgcgga gtctctctta tttttttgaa
     3601 tagatcccga ttttcaccag gataatactt atctaatcca catgctgtga cagcaattgt
     3661 cggagcttgg tattgaagag ccgcgacatg aacgctacgg tctattcctc gggcaatacc
     3721 gctaacaagc gcatgattat acccagcaat cccgcaacag agtcttgctg ttacatcctc
     3781 ggacttatta ttgctatttc ggcttcccac aatggctatg gccggtttcg cgcaaaattc
     3841 tccctgcctg tataaaacca gtggcggatc agcaatgcgg gacagcagaa gggggtactg
     3901 actgcttgtc attggaatga tatctacacc gattttggca caacgattta aaattttatc
     3961 agccgcctta aacacatcgg ctccgtaaat tttctttata tgagccacgc aatccctatg
     4021 ttcgggtcta acatcctcaa agatagctt
//
LOCUS       DAOTGZ010000242         4028 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_3064
            NODE_26435_length_4028_cov_4.421596, whole genome shotgun sequence.
ACCESSION   DAOTGZ010000242 DAOTGZ010000000
VERSION     DAOTGZ010000242.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868088
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 4028)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4028)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:22:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,785
            CDSs (total)                      :: 2,751
            Genes (coding)                    :: 2,732
            CDSs (with protein)               :: 2,732
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 12 of 19
            Pseudo Genes (internal stop)      :: 5 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4028
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_26435_length_4028_cov_4.421596"
                     /isolate="AMR_MDS_3064"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1311
                     /locus_tag="P5123_12215"
     CDS             <1..1311
                     /locus_tag="P5123_12215"
                     /inference="COORDINATES: protein motif:HMM:NF037997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005436 - sodium:phosphate symporter
                     activity [Evidence IEA];
                     GO_function: GO:0015114 - phosphate ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0035435 - phosphate ion transmembrane
                     transport [Evidence IEA];
                     GO_process: GO:0044341 - sodium-dependent phosphate
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Na/Pi symporter"
                     /protein_id="HRX17071.1"
                     /translation="FLYLFSRNEKVKYIAFTIMGVGMVFFGLELMKDGFKPIRLLPEF
                     EAMFSMVHATSYFNVLLIALIGCLLTMIVQSSSATLGITIGLAQTGVIGFETAAALVL
                     GENIGTTITAMFASIGTNTNAKRAAYFHTLFNLTGVLWITALFQLYLPLIRSIVGHDP
                     NTMVLKNGNETYPYITAGIASVHTVFNIVNVILFIPFTRKIATLLTKVVKESKVKTTM
                     ITKLQQSLLTSPFAAVQQTKYEVIKMEQVTRGMMNTLRAIINGEKKSQSTINIIFRDE
                     EKLDIMQSEITEFLTDLMSEPLPMEIAEECKEHLRMSDELESVSDYITQILKLLLRLE
                     DNDTKLSDEQVANIIKLHDEVKNFFNLVRDCEKNQTIQVEYDKICAASDDLTALIRTY
                     RNDHWEKASMTKMLPLVNTAYTDILQSYRKIKNHMLNSVEVQAL"
     gene            complement(1354..2928)
                     /locus_tag="P5123_12220"
     CDS             complement(1354..2928)
                     /locus_tag="P5123_12220"
                     /inference="COORDINATES: protein motif:HMM:NF012245.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein"
                     /protein_id="HRX17072.1"
                     /translation="MSFELDQKLEKVIIKARFFFVLLFVLAGTSSLTGGSPIAVWGSL
                     YFLGAIAAFNSIVSSILLKQNRLTLSIIIITMIIDTLIPFFMKIAFSLDPELGLGYGL
                     KEPGSYIVYYFFLIIHALRYNRSLIILSGFIAFTSYLGIVIYSVTLGGLSFVRSIEGF
                     TDLKLLRTANELPKILLLIGFVYYLQIMARFSSNNIKELKLSRINADETLVKMKKLFG
                     TVQTSSEELVSDSQLLKESAHYIYTILESFHGLMNEINVLTQDATDNLHHIKSSSSFQ
                     YDKVKANFKKITEIAELMEHINNDSSVHHINAKEALKLAELNERNIQDSLNAINEMKL
                     NSDKIEEISGTISEIADKTNLLSLNAAIESARAGEHGKGFAVVAEEISKLAAMSIDSS
                     KEISLIIKGTVTNIDHTSATIENLAQFLHKIGSFVRDNSKFMKELNEKTYHEYNESKD
                     LFDSTAAVDQAAKDVIEKAEEQIKFVNNIDNWMENFSSMCDKVTSNISHIREISAKLD
                     NQSVTLNSLVKTDQTN"
     gene            complement(3135..3599)
                     /locus_tag="P5123_12225"
     CDS             complement(3135..3599)
                     /locus_tag="P5123_12225"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF025054.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HRX17073.1"
                     /translation="MELTIIKLNKLMAKEVSSLVKETFDNYVAKDYTKEGIKTYHNYI
                     SPEAMLERSNNGALILVAIYNDSIAGMIEIINNNEISLFYVDSKWQKLGIGKELFIEA
                     IRRVKNNHLDSLTVKASVFAEKVYLSLGFTRTGEETIDNGMTVIPMIYHIGE"
     gene            complement(3601..>4028)
                     /locus_tag="P5123_12230"
     CDS             complement(3601..>4028)
                     /locus_tag="P5123_12230"
                     /inference="COORDINATES: protein motif:HMM:NF013722.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="MaoC/PaaZ C-terminal domain-containing protein"
                     /protein_id="HRX17074.1"
                     /translation="TKLEKRRNPKTVYENNSGITNYDQFPINTIKNHTKQYAKLSRDK
                     NPIHTSRLFAALAGLPSPIMHGMHTMGLVSAALIEKYSESGWNGLDYVSVRFSRYVIP
                     GETITINSYCSSRGRIDFAAQNSNGSKILTDGCLQIKEI"
BASE COUNT         1211 a          731 c          772 g         1314 t
ORIGIN      
        1 tttctatatc tctttagccg aaatgaaaag gtcaaatata tagcttttac gattatggga
       61 gtcggaatgg tcttctttgg tcttgaactc atgaaagacg ggtttaaacc aataagacta
      121 ttacctgagt tcgaagcaat gttctcaatg gtgcatgcga catcatattt taacgttctg
      181 cttatcgcgc taataggttg tttgctaacc atgattgttc agtcttcttc ggcgacattg
      241 ggtattacca taggtcttgc ccagactggt gtaatcggtt ttgagactgc tgcggcactt
      301 gttcttggtg aaaatatcgg tacgactata actgcgatgt ttgcgtccat aggcacaaat
      361 acaaatgcta agagggctgc atattttcat actctgttta atttaacggg agtattatgg
      421 atcacggctc tgttccagct ttatcttcca ctaatacgtt ccattgtggg gcatgatccg
      481 aacacgatgg ttctaaaaaa cggaaatgag acctatccct atattaccgc cggtattgcc
      541 tctgtacaca ctgtgtttaa catagttaac gttattctct ttattccttt tacccgaaaa
      601 atagctactc ttttgactaa agttgttaaa gagtcaaagg taaaaaccac aatgattacc
      661 aagcttcagc agagcctcct tacttcaccc tttgccgcgg ttcagcagac caaatatgaa
      721 gttattaaaa tggagcaggt tacccgcgga atgatgaata ctctacgggc aataatcaat
      781 ggagaaaaaa agtctcaatc gaccattaat ataatatttc gtgacgaaga aaaactggac
      841 attatgcaat ccgagattac cgaattcttg accgacctta tgagcgaacc tttaccaatg
      901 gagattgcag aagagtgcaa agaacatctg agaatgagtg atgagctaga gtcggtaagc
      961 gattatatta cacagatatt aaagcttttg cttagacttg aggataacga cactaagctt
     1021 tcagatgagc aggtagccaa tattattaag cttcacgatg aggtgaaaaa cttttttaat
     1081 cttgtcagag attgtgaaaa aaatcagacc atacaggtcg agtacgataa aatttgtgct
     1141 gcaagtgatg acctaaccgc acttatccga acctatcgta atgaccactg ggaaaaggca
     1201 tccatgacaa aaatgttgcc tctggtaaac acagcttata ccgatattct acagtcatat
     1261 cgaaagatta aaaatcatat gcttaatagt gtagaggtgc aggcgctata aaaataaggg
     1321 atatttatgg gcaagagtca tcttgccctg gtttcaattt gtctgatccg tcttaacaag
     1381 actgtttaag gtaacagact ggttgtcaag tttagcggaa atttcgcgaa tatggctaat
     1441 attacttgtt actttgtcgc acatcgatga aaagttttcc atccagttgt ctatgttgtt
     1501 aacaaacttg atctgttcct ctgccttttc gattacatct ttagcagcct gatcaaccgc
     1561 tgctgtcgaa tcaaaaagat ctttgctctc attatattca tggtatgttt tttcgttaag
     1621 ctctttcatg aatttggaat tatcacgcac aaagctgcct atcttatgta aaaactgcgc
     1681 cagattttct attgtagccg atgtatgatc aatatttgtt acggttccct ttattattaa
     1741 agatatctct tttgaagaat caatactcat tgctgctaat ttcgatatct cttcggcaac
     1801 aacagcgaaa cctttgccat gctcgcctgc tctggccgat tctatcgcgg catttaatga
     1861 aagcagattt gttttgtcgg caatttcgct gatagtgccg gaaatctctt caatcttgtc
     1921 cgaattcaac ttcatttcgt taatggcgtt cagagaatcc tgaatattcc gttcattaag
     1981 ttcggcaagt ttgagagcct cttttgcatt gatatgatgt actgaactgt cgttgttaat
     2041 atgttccata agttcggcaa tttcggtaat ttttttaaag ttggctttta ccttgtcata
     2101 ctgaaaagat gagctgctct ttatatggtg aaggttgtcg gtcgcgtcct gcgttaaaac
     2161 gttgatctcg ttcataagtc cgtgaaagga ttcgagtatt gtgtaaatat agtgagcaga
     2221 ttctttgaga agctgactat cactaaccag ttcttcagag ctagtttgaa ctgtgccaaa
     2281 cagcttcttc atctttacga gtgtttcatc ggcattgatt ctagacagtt taagttcttt
     2341 tatgttgttg gaagagaacc gcgccatgat ttgtaaatag tagacaaagc caataagcaa
     2401 aagaattttt ggaagctcgt tggcggtgcg tagaagctta agatcagtga agccttctat
     2461 cgaacgtaca aaactcagac ctcctagcgt cacactgtat ataacaatcc caagatacga
     2521 tgtaaaggcg ataaagccgg acaagataat aagtgatctg ttataacgga gagcatgaat
     2581 aatcaggaaa aaatagtata caatataact accgggttct tttagtccgt agccgagccc
     2641 cagctcggga tctagagaaa aagcgatttt cataaaaaaa ggtatcaggg tgtcaataat
     2701 catggtgata atgataatcg atagggttaa cctgttttgt tttaatagta tggaagaaac
     2761 gatagagtta aacgcagcaa ttgcgccaag aaaatagagt gatccccaaa cggcgatggg
     2821 tgatcctcca gttaatgagg atgtgcctgc cagaacaaac agcaagacaa agaaaaaccg
     2881 ggctttaatt atgacctttt caagtttttg atctagttca aaactcatat tgggtttcct
     2941 tgaataaatt aaaaaggata taatgacaga aatgtaacaa acatgttaaa tattgttgta
     3001 ataaaaaaaa tttaatataa agtagatatg ctgaataaac ttcaattaag ataattaatc
     3061 gacttcagct taattgtcaa atatattttt ttttatataa atttacgcaa ggttttttta
     3121 acgatacaca cgagtcattc gccgatatga taaatcattg gaataaccgt cattccgttg
     3181 tctattgttt cttctcctgt ccgtgtaaat cctaggctta aataaacttt ttcggcaaaa
     3241 acagaagctt tcacggttaa tgaatccaga tggttatttt tgactctgcg tatcgcctca
     3301 atgaaaagct ctttacctat tccaagtttt tgccatttac tgtctacgta aaatagagaa
     3361 atttcgttat tatttattat ttcgatcatt ccggcaattg agtcgttata tatagcgaca
     3421 agaatcaaag caccattatt tgatctttcg agcatggctt caggtgaaat ataattgtga
     3481 tatgtcttta tcccttcttt tgtataatct ttggctacat aattatcaaa agtctcttta
     3541 accagagagc tgacttcttt tgccattaat ttatttagct ttattattgt aagttccata
     3601 tcagatctcc ttaatttgca ggcagccgtc agtaagaatt ttcgatccat tgctgttttg
     3661 agctgcaaaa tcgatacgtc ctcttgagct gcaataagaa ttaattgtaa tagtttcacc
     3721 gggaataacg tatcgagaaa atcttactga tacatagtct agaccgttcc aacctgattc
     3781 tgaatacttt tcgattaatg cagctgatac aagtcccatc gtatgcatgc cgtgcattat
     3841 tggtgatgga agtcccgcta aagcggcaaa aagtcgtgat gtgtgaatcg gatttttatc
     3901 ccttgatagt tttgcatatt gtttagtgtg attttttatt gtattaattg gaaattgatc
     3961 ataattagta atgccgctat tgttttcata aactgttttt ggatttctcc ttttctcaag
     4021 tttggtaa
//
LOCUS       DAOTGZ010000243         4010 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_3064
            NODE_26590_length_4010_cov_5.464728, whole genome shotgun sequence.
ACCESSION   DAOTGZ010000243 DAOTGZ010000000
VERSION     DAOTGZ010000243.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868088
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 4010)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4010)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:22:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,785
            CDSs (total)                      :: 2,751
            Genes (coding)                    :: 2,732
            CDSs (with protein)               :: 2,732
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 12 of 19
            Pseudo Genes (internal stop)      :: 5 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4010
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_26590_length_4010_cov_5.464728"
                     /isolate="AMR_MDS_3064"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1876)
                     /gene="feoB"
                     /locus_tag="P5123_12235"
     CDS             complement(<1..1876)
                     /gene="feoB"
                     /locus_tag="P5123_12235"
                     /inference="COORDINATES: protein motif:HMM:NF007105.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0015093 - ferrous iron transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006826 - iron ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe(2+) transporter permease subunit FeoB"
                     /protein_id="HRX17075.1"
                     /translation="MNKRNIALVGNPNCGKTTIFNVLTGGKIKTGNWPGVTVEKREGE
                     FKHKGESANIIDLPGIYSFSSGSEDEIIARDYVLSSEPSLIMNIVDATNLERNLYLTI
                     QLIEMKVPIVLVVNMIDCAIKNCIKIDLKTLGKKLGIPVIGISAIKTDDTVLLKNFLV
                     NHLDHPNISSAIISYPNEIETVIDNWSSQLTHCAQKMTIDSRWIAIKLLENNDSIRQQ
                     VINNSDLTVKEISKEQTYIEQILEDSPDIICADYRYGFIHSIARTVIERKQTRRTISD
                     IIDRFVLNRFLGIPIFFLIMYLIFWITITVGGAFIDFFDILTGTVFVEGFGALLNYLK
                     FPDWLITFLANGIGAGIQTVSTFVPIIFFMFLMLSILEDSGYMARAAFVMDRFMRLLG
                     LPGKAFVPLLVGFGCTVPAIMATRTLENRKERYLTVFMAPFMSCGARLPVYALFTAAF
                     FPLNGGLIVFSLYMSGIFFAVLTGLLLKKTLFKNSDSYFVMELPPYHSPRPFHLLTHT
                     WDRLKSFILRAGGVIIIVVTLLSIFNSIGTDGSIGNENTDKSILASIGKTITPVFKPM
                     GIEEKNWPATVGIFTGIFAKEAVVGTLNSLYATITDNKIEVNQEEENFSFSNGVIKAF
                     T"
     gene            complement(1869..2111)
                     /locus_tag="P5123_12240"
     CDS             complement(1869..2111)
                     /locus_tag="P5123_12240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002725872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046914 - transition metal ion binding
                     [Evidence IEA];
                     GO_process: GO:0006811 - monoatomic ion transport
                     [Evidence IEA];
                     GO_process: GO:0055072 - iron ion homeostasis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FeoA family protein"
                     /protein_id="HRX17076.1"
                     /translation="MKKTINQIKMGDCVTIEGYNKTDRIYREKLLSMGLTPGTNFRLV
                     SRAPMGDPVEIEIRGFRLTLRAHEAEILVLQGDNDE"
     gene            complement(2162..2410)
                     /locus_tag="P5123_12245"
     CDS             complement(2162..2410)
                     /locus_tag="P5123_12245"
                     /inference="COORDINATES: protein motif:HMM:NF015956.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046914 - transition metal ion binding
                     [Evidence IEA];
                     GO_process: GO:0006811 - monoatomic ion transport
                     [Evidence IEA];
                     GO_process: GO:0055072 - iron ion homeostasis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FeoA family protein"
                     /protein_id="HRX17077.1"
                     /translation="MNQHSFIIPLILAEESKELCVYELKGGNFFRDKCMSQGILPGHN
                     IILVKKCIKGPYIINVRNTNIILSQNIVRKILVTNNDL"
     gene            complement(2580..3023)
                     /locus_tag="P5123_12250"
     CDS             complement(2580..3023)
                     /locus_tag="P5123_12250"
                     /inference="COORDINATES: protein motif:HMM:NF014762.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent transcriptional regulator"
                     /protein_id="HRX17078.1"
                     /translation="MILSSSLEDYLEAIYDIEITGHEVRITDIGTKLGVEKSSVNYAV
                     NKLCNLKLVLHEPYTDIKLTSAGRKTAKLIKNRHDIIKKFLKNVLLLSEEISDNDACR
                     MEHILSPETFENLSKFVDFYENLKSINNKDIDQLFRETIVNGQKN"
     gene            3337..>4010
                     /locus_tag="P5123_12255"
     CDS             3337..>4010
                     /locus_tag="P5123_12255"
                     /EC_number="2.7.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013313081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6-phosphofructokinase"
                     /protein_id="HRX17079.1"
                     /translation="MKKMDTTIKNLGEKTIPSPLQARFPLTSSIFVHGKMRILYNVLK
                     RDVIAEIEAGEINSFEKGGPRKLIYHDPSKVKAAVVTCGGLCPGINDVIRAIVMESFY
                     SYGINNIVGIPYGYNGLLPQNQYAVIPLDPKKVDNIHNAGGTILGSSRGGTDDISALV
                     DALERMNINILYTIGGDGTQRGAHKIATIARERGYKLSVIGIPKTIDNDISFVERTFG
                     FETAFS"
BASE COUNT         1235 a          685 c          739 g         1351 t
ORIGIN      
        1 aagtaaatgc tttaataaca ccgtttgaaa aactaaagtt ttcttcttct tgattaacct
       61 ctattttatt atcagttatt gttgcatata aagagtttaa tgttccaaca actgcctctt
      121 tggcaaatat tccagtgaat attccgactg ttgccggcca gtttttttct tcgattccca
      181 ttggcttaaa aactggtgtg attgttttgc caattgaagc aagaatcgat ttatcagtat
      241 tctcattacc aattgaacca tctgtaccaa ttgaattgaa aattgataat aatgtgacaa
      301 caattattat cacaccaccg gcacgtaaga taaaactttt gagcctgtcc catgtgtgag
      361 ttagcaggtg gaatggacgc gggctgtgat atggtggaag ctccatcaca aagtaagagt
      421 cgctgttttt aaaaagtgtt tttttcaata atagtccggt taataccgcg aagaagattc
      481 ctgacatgta taatgaaaaa acaatcaagc caccgtttag tggaaaaaaa gcagcagtaa
      541 atagagcgta tacggggagt cttgctccgc aagacataaa cggtgccata aagactgtta
      601 gatatcgttc ttttctgttt tccaaagttc ttgttgccat tattgcaggt actgtgcatc
      661 cgaatccgac aagaagtggg acaaacgctt ttccaggaag tcctaagaga cgcatgaacc
      721 gatccattac aaaagccgcg cgagccatat aaccgctatc ttcaagaatg gacaacatta
      781 aaaacatgaa gaatataatt ggcacaaagg ttgacacagt ttgtatgccc gcgccaatac
      841 cgtttgccag aaatgtaatg agccagtcag gaaattttaa gtaatttaat agagcaccaa
      901 agccttcaac gaaaactgtt ccagtgagaa tatcaaaaaa atctatgaaa gctccgccga
      961 cagtgattgt aatccagaag attaagtaca taatcaaaaa gaagattggg attccaagaa
     1021 aacgatttaa aacaaatcta tcgataatgt ccgatatggt tcgtcgtgtt tgcttgcgct
     1081 caataacggt tctagctata gagtgtataa agccgtaacg atagtccgcg caaattatgt
     1141 ctggcgagtc ttcaagtatc tgttcaatat aagtttgctc tttactaatt tcctttacag
     1201 ttaaatcact gttattgata acctgttgtc ggatgctgtc attgttttcc aaaagtttta
     1261 ttgcgatcca acgtgagtct attgtcattt tttgagcaca gtgcgtgagt tgagaactcc
     1321 aattgtcaat tacagtttcg atttcgttgg ggtatgatat gatagcagag gatatgttgg
     1381 ggtgatccaa gtgatttacg agaaagtttt taagtaaaac tgtatcgtca gttttaatgg
     1441 cgctgattcc tataactgga attccaagct ttttacctag tgtctttaaa tcaatcttta
     1501 tgcagttctt tatcgcgcag tctatcatat tgacgactaa aacaatagga actttcattt
     1561 cgataagctg aatcgttaga tacaggttgc gttcgagatt tgttgcgtcg acgatattca
     1621 taattaatga gggttcggag cttaatacgt aatcgcgtgc gattatttcg tcttcagaac
     1681 cggatgaaaa agagtatatt cccggcagat caataatatt tgctgattcg cccttgtgtt
     1741 tgaattctcc ttctcttttt tcgactgtga cgccaggcca gtttcctgtt tttatttttc
     1801 cacccgttaa aacgttaaat attgtggttt taccgcagtt gggatttcca actaatgcta
     1861 tatttcgttt attcatcgtt atctccttga agtactaaga tttcggcctc atgtgctctt
     1921 aaggttaaac gaaagcccct aatttcaatt tctaccggat ctcccatagg ggcacgcgaa
     1981 acaagtctaa aattcgttcc cggggttaaa cccattgaaa gaagtttttc tctataaatt
     2041 ctatctgttt tgttgtagcc ttcaatggta acgcaatctc ccatcttgat ttgattaatt
     2101 gtttttttca ttttttacct cactaatatg ttagatggct cgaacatatt attaaatttt
     2161 tttacagatc attatttgtt actaaaattt tacgcacaat attttgacta agtataatat
     2221 tggtgtttct tacattgatt atataaggac ctttgataca ttttttaacc aagataatat
     2281 tgtgacctgg aagtattccc tggctcatgc acttatcacg gaagaaatta ccacctttaa
     2341 gttcgtaaac acatagctct ttgctttctt ctgcaagaat cagtggaatg ataaaggaat
     2401 gttgattcaa tttgcacccc caaaagttat attgctctaa caaattaaca aaaaaaatgt
     2461 ttaatatgtt tttaaacggt gtagccgttc tataaacggt taacttaaaa agctccgtat
     2521 gactaagtaa ttatctaaaa gtatgtgaaa atattttatg aatacggcgt tatataaact
     2581 taattctttt gaccgtttac gatagtttct ctgaacaatt ggtcaatatc cttgttgttg
     2641 atagatttta aattttcata gaaatcaaca aatttcgaaa ggttttcgaa tgtttctgga
     2701 cttaaaatat gttccattcg acaagcatca ttgtcagaaa tttcttcaga gagtaatagt
     2761 acgtttttta aaaatttctt tataatatca tgtctgtttt ttattaactt tgccgtttta
     2821 cgccctgcgg aagttaattt aatgtcagta tagggctcgt gtagaacaag cttaaggtta
     2881 cagagtttat taacggcata gtttaccgat gatttttcaa caccaagttt tgtaccgata
     2941 tcggtaattc taacttcgtg tccggtaatt tcaatatcat aaatagcttc cagataatct
     3001 tcaagacttg aactaagaat catttattac ctctcaatgt tagacaactc taacattgag
     3061 agctaatatt gcaacttttt ttttgtaatc taattttttt gaagtaaata ttttaatgtt
     3121 aagtgcttac ttaatattct tgacattggt aagtataata cgctttttat gtaatgttgt
     3181 aattatgttt gcgcttatat cgtagaatgt tgttaactac caaaaaggac aaaataaaat
     3241 ttattatctg aggatagtat tttattaaac agttcgaata aagcaaaccg gttgcatttt
     3301 gttttggcaa atatgataaa gaagggagaa tataaaatga aaaaaatgga tacaacgata
     3361 aagaatcttg gtgaaaaaac tattccgtct cctcttcagg caaggtttcc gttaacatca
     3421 agtatatttg ttcacggtaa gatgagaatt ctttacaatg ttttaaaacg tgatgttata
     3481 gcagaaattg aggctggaga gataaattct ttcgaaaaag gcggacccag aaagcttatt
     3541 tatcatgatc cgtcaaaagt taaagcagcg gttgttacct gcggaggcct atgtcctgga
     3601 ataaacgatg ttattcgcgc tatagtaatg gagtcgttct attcttacgg tataaataat
     3661 attgttggta ttccttatgg ttataacggg cttcttccgc aaaatcagta tgctgttatt
     3721 ccgcttgatc ctaaaaaagt cgataatata cataatgccg gcggcactat cctgggctct
     3781 tcgcgcgggg gtacggatga tattagtgct ctggtagatg ctctggagcg tatgaatata
     3841 aacatacttt atacaattgg cggtgatgga acacagcgcg gtgcccataa aatagcaacg
     3901 attgcccgtg aacgtggata caaactctct gtaatcggga ttcctaaaac cattgataac
     3961 gatataagtt ttgtagaaag gacatttggt tttgaaacag ctttttcgga
//
LOCUS       DAOTGZ010000244         3940 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_3064
            NODE_27228_length_3940_cov_4.382239, whole genome shotgun sequence.
ACCESSION   DAOTGZ010000244 DAOTGZ010000000
VERSION     DAOTGZ010000244.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868088
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 3940)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3940)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:22:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,785
            CDSs (total)                      :: 2,751
            Genes (coding)                    :: 2,732
            CDSs (with protein)               :: 2,732
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 12 of 19
            Pseudo Genes (internal stop)      :: 5 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3940
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_27228_length_3940_cov_4.382239"
                     /isolate="AMR_MDS_3064"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..695)
                     /locus_tag="P5123_12260"
     CDS             complement(<1..695)
                     /locus_tag="P5123_12260"
                     /inference="COORDINATES: protein motif:HMM:NF013069.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HRX17080.1"
                     /translation="MKKILSFLIDQKMFMGLLIFMALVIGYMSLTSRNREAFPEVAFD
                     MVTITTIYPGGSPDEIETLITIPIEKKIREVDGIDKVRSYNIENVSVLAIYLDEDYSD
                     PDAAVQNIKDAVDTVTDLPSGAEAPAVKEIKIDKTTVLRVALFANNDDVSYAKLRETA
                     KQMEDFLYEFKGVAEVTKEGYLEKEYLVEVNPAQLEFYRFAINDVVNTLKNRNMDIPG
                     GPLRIGSDEYVLR"
     gene            complement(996..1763)
                     /locus_tag="P5123_12265"
     CDS             complement(996..1763)
                     /locus_tag="P5123_12265"
                     /inference="COORDINATES: protein motif:HMM:NF021386.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2087 domain-containing protein"
                     /protein_id="HRX17081.1"
                     /translation="MNPIFLESSLTITNLKTGWIFDENAEEFKCIFCSQCFRQGIIYK
                     NKSNFIDAKRAVIDHIKEAHGSSFDVLVNLCKKDNGLSDIQKLIMTGMYRGLSDDKIA
                     RMASSPRALSTIRNHRFTLREKYREARIFTALMEIIEENAAKNPEENFVDYHNALPVS
                     DGRAVITENEREKLINKHMDTKNRILKKFPKKQKEKLVLLREIALWLEKERIYTEREL
                     NDVLKSIYHDYAEIRRYLIEYRFMERKADGSEYRVII"
     gene            complement(2136..3233)
                     /locus_tag="P5123_12270"
     CDS             complement(2136..3233)
                     /locus_tag="P5123_12270"
                     /inference="COORDINATES: protein motif:HMM:NF024895.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane beta-barrel protein"
                     /protein_id="HRX17082.1"
                     /translation="MKKLTIVTALFLTLLCTTAYAADKSKLEWGKMPGVARYDVEVRN
                     TMTGSIKRYTTTEDHIWADIGFGFYEMRVTAFNKAGKEVTATRWIPLTIMKTYQPEVN
                     KLLVKPNGSNIVQGDNYYQQTKVSISKNGSPLNVRMTGGQYPKEVTFAAPLQKNTKYS
                     LLVENPGDYRAKRIAHVFNDSSTMLFNNEDEYTRYFSDFFAQASINYQILMNSYWKDV
                     YEAGLNSGTISVGYHFFSWFGATLQTDFSRYTTTNTAEGDLDITNNYITLQPGIFFSY
                     KNKDYIPYIFGSAGVSYSWLDVKTPINNIEKSSTDLVYSGGAGCKYFVYKSIFTDLSL
                     SYSSVRLEGKTMDSVSIRLGFGSYFKIRLFP"
     gene            complement(3235..>3940)
                     /locus_tag="P5123_12275"
     CDS             complement(3235..>3940)
                     /locus_tag="P5123_12275"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRX17083.1"
                     /translation="TPLSNSYSEGVTHFQLKEGLGLTLISPTINSHFKAGSDITFIWR
                     DQSAALNYQLQISEDNSFKNPVIESKVSTFSDTLRLAEPGIYFARVNILDPSDKTLVF
                     GDIRKFSIPIDLSLPSFNESDMPSDIDTIVTPSVEVSWNSVPGADRYQASLSKAGEAM
                     SETVISTITTTSTSASFSNTGRLTPGRYCIKITALKKEQELLTAVSGEVKHYFNASRG
                     KGLEIKTPETIFILVK"
BASE COUNT         1107 a          705 c          833 g         1295 t
ORIGIN      
        1 atacgtaaga catattcgtc gctgcctatt cttaacgggc cgccgggtat atccatgttt
       61 ctgttcttta aggtgttcac aacatcgttg atggcaaaac ggtaaaactc aagctgtgcc
      121 gggttgacct ctacaaggta ctctttctca agatacccct ctttggtaac ttcggctaca
      181 cctttaaact cgtacaggaa atcttccatc tgtttagcgg tttcgcgcag tttcgcgtag
      241 cttacgtcgt cgttgttggc aaaaagagct acgcggagaa cggttgtctt gtcaattttt
      301 atctctttaa cggcgggagc ttccgcgccg gatggaaggt cggtaacagt gtcaaccgcg
      361 tcctttatat tttgaactgc tgcatcggga tcggagtagt cctcatcgag atatattgca
      421 agcaccgaca cgttttctat attgtaactt cgaactttgt cgattccatc gacttccctt
      481 atcttttttt cgataggtat agtaatcaga gtctcaattt cgtcgggaga gccgccggga
      541 taaatggttg tgatggttac catgtcaaaa gcgacttcag ggaatgcctc cctgttgcga
      601 ctagtaaggg acatgtagcc tattacaaga gccataaaaa taagaagccc cataaacatc
      661 ttctggtcga taagaaatga gagtattttt ttcattaaga gtaacctcca taagaataaa
      721 cagttcagca gtatataatc ggtaacaagc tgtatggatt tatcttgcca tattattggg
      781 aatagatcct taaatagatg gcgataaaat taaaaatatg tattttctca agggtctgtt
      841 tgatgatatt aataatcata ttgatacaga tccataatgg ggatattata gatagctgtc
      901 aattttttgc tacacaaaaa atcaggtttt tgtagttttt agggtttttt tgggtgttgg
      961 cttcataccg aagccgtttt tttcgtattc ttttgtcaaa ttatgacacg gtattcggat
     1021 ccgtcagctt tacgctccat aaatcgatat tcgatcaggt accggcgtat ttcggcgtaa
     1081 tcatgataaa ttgatttcag cacatcattg agctctcttt cggtatatat tcgctctttc
     1141 tcaagccata gagctatttc ccgtaaaaga accagtttct ctttctgttt cttagggaat
     1201 tttttcaaaa tacggttttt tgtatccata tgtttgttta taagtttttc acgctcgttt
     1261 tccgtaataa ctgctcttcc atcactaact ggcagggcgt tatggtaatc gacaaaattc
     1321 tcttcaggat tttttgcagc attctcttcg attatttcca taagggctgt aaatattctt
     1381 gcttcgcgat atttttcacg aagtgtgaag cgatggttac gtatggtcga cagcgctcgt
     1441 ggtgaagagg ccatgcgtgc gattttatca tcggataatc ccctgtacat tcctgtcatg
     1501 atgagttttt gaatatcaga gagtccgtta tcttttttgc ataagtttac aagtacatca
     1561 aacgatgaac catgagcctc ttttatatga tcaataacag ctcgttttgc atcaatgaaa
     1621 ttggatttgt ttttataaat aattccctgc ctgaagcatt gtgagcagaa tatgcatttg
     1681 aattcttctg cattttcgtc aaaaatccaa cccgttttaa gattggtaat tgttagggat
     1741 gattcaagaa atataggatt cattgttaac ctcgatgatc tagatatttt gtgataatgc
     1801 ttgattttat ctaaacaata tatgaatatg tttggataaa tgcaaacaaa atttattaaa
     1861 gtttgctaaa aattacggga tatttgataa gttttaataa atatagcttc tatccattac
     1921 tggaaaaaat agtgatttga ttgaaggaga tgaaatagtg atttgattga aggaaatgaa
     1981 atagtgattt gattgaagga gatgaaatag tcatttgatt gaaggagatg aaatagtcat
     2041 ttgattgaag gagattaaaa atagtgattt gattagaaga gaataaaaaa tgatcagata
     2101 acagaattat ctgatagaga ggcaggagtt ctctatcagg gaaataaacg gattttgaag
     2161 tacgacccaa aacctaggcg tattgacacg gaatccatag ttttgccttc gaggcgtacc
     2221 gatgagtagc ttaaagagag gtcggtaaat attgatttat aaacaaagta cttgcaacca
     2281 gcacctccgc tgtaaaccaa gtcggtggaa ctcttttcaa tattgtttat tggcgtttta
     2341 acatcaagcc atgaatatga aacccccgct gaaccgaata tataaggaat atagtccttg
     2401 tttttgtaac tgaaaaatat tccaggctgc agggttatgt agttgtttgt tatatccagg
     2461 tcaccctcgg cagtattagt ggttgtatag cgggaaaagt cggtttgaag ggttgctcca
     2521 aaccatgaaa aaaagtgata accgacactt attgttccgg aatttaaacc ggcctcgtat
     2581 acatctttcc aatagctgtt cataagaatc tggtaattta tagatgcctg tgcaaaaaaa
     2641 tcagagaagt aacgtgtata ctcatcttcg ttattaaaaa gcatggtgct agagtcgtta
     2701 aagacgtggg caattcgttt agctcgataa tccccagggt tttcgaccaa tagggagtac
     2761 ttggtatttt tttgcaacgg agctgcgaag gtcacctctt tggggtactg tccacctgtc
     2821 attctcacat ttaatggaga gccgttcttg ctaatcgaaa ccttggtctg ctgataatag
     2881 ttatcaccct gaacgatatt tgacccgttt ggtttgacta ataatttatt aacctcgggc
     2941 tgataggttt tcatgattgt aagaggaatc caccttgttg cggtcacctc tttgccagct
     3001 ttattaaaag cagttacacg catttcgtag aagccgaatc cgatgtcggc ccagatgtga
     3061 tcttctgttg tagtatagcg ttttatgctg ccagtcatag tgttgcgtac ttcaacatca
     3121 taacgggcaa cacctggcat tttgccccat tccagttttg acttatctgc agcgtaggca
     3181 gtagtgcata gaagagttaa gaatagtgct gtaactattg tcagtttttt catgttattt
     3241 taccaatata aagatggttt ctggagtttt tatttcgagg cctttgcccc ttgaagcatt
     3301 gaaatagtgt tttacttcac cggatacggc ggttaagagt tcttgttcct tttttagagc
     3361 cgttattttg atacagtatc ggccaggagt taatcgacct gtgtttgaaa atgaagcaga
     3421 tgtcgaagtt gtagtaatgg tactaattac agtctctgac atggcttcgc ccgcttttga
     3481 aagggaagcc tgatatctat ctgcgcctgg aacgctgttc cagctgactt ctactgatgg
     3541 tgtgactatt gtatcaatat cggacggcat atcgctttcg ttaaacgatg gcaaggacag
     3601 gtcaattgga atagaaaact ttctaatgtc accaaaaaca agcgttttgt ctgacggatc
     3661 cagaatattg actctggcga aatagatgcc cggttcggca agtctcaaag tgtcggaaaa
     3721 ggtcgatacc ttactttcaa ttacaggatt tttaaacgag ttatcttcag aaatttgcaa
     3781 ctgataattg agtgccgcgg attgatctct ccagataaat gttatgtcag agccggcttt
     3841 gaagtgagaa tttatggttg gggaaataag tgtaagtccc aatccctctt ttagttgaaa
     3901 atgtgtcacc ccttcgctat agctgttaga tagaggtgtg
//
LOCUS       DAOTGZ010000245         3939 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_3064
            NODE_27239_length_3939_cov_4.114058, whole genome shotgun sequence.
ACCESSION   DAOTGZ010000245 DAOTGZ010000000
VERSION     DAOTGZ010000245.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868088
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 3939)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3939)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:22:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,785
            CDSs (total)                      :: 2,751
            Genes (coding)                    :: 2,732
            CDSs (with protein)               :: 2,732
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 12 of 19
            Pseudo Genes (internal stop)      :: 5 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3939
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_27239_length_3939_cov_4.114058"
                     /isolate="AMR_MDS_3064"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            140..427
                     /gene="hgcB"
                     /locus_tag="P5123_12280"
     CDS             140..427
                     /gene="hgcB"
                     /locus_tag="P5123_12280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005985173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0042192 - methylmercury biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mercury methylation ferredoxin HgcB"
                     /protein_id="HRX17084.1"
                     /translation="MRQKYITKVLSLELNNKLCTGCGMCATVCPHNVFDIMEGKAAAS
                     RKQYCMECGACKLNCRFNAISVNSGVGCGYAVIMGAIKGTEPDCGCSSGCC"
     gene            945..1292
                     /locus_tag="P5123_12285"
     CDS             945..1292
                     /locus_tag="P5123_12285"
                     /inference="COORDINATES: protein motif:HMM:NF024252.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HRX17085.1"
                     /translation="MTLGEKIKHLRKEQNISQTELAKIIGVHQSHIGRYERDESTPTA
                     YVIKKLAEVLKVSADYLLSEEEEEIAASFTSDKELLQHFQDISRLADPDKQFIKRMIK
                     MAINDAKIKEMAS"
     gene            1303..1503
                     /locus_tag="P5123_12290"
     CDS             1303..1503
                     /locus_tag="P5123_12290"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRX17086.1"
                     /translation="MQLDITKDELYTIIKSAVRDVLKEDKVKHILDSAPYVSDEEMED
                     IVSIHGDAPGPKEVSRTEILDL"
     gene            1500..1766
                     /locus_tag="P5123_12295"
     CDS             1500..1766
                     /locus_tag="P5123_12295"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0008657 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) inhibitor activity
                     [Evidence IEA];
                     GO_function: GO:0042803 - protein homodimerization
                     activity [Evidence IEA];
                     GO_process: GO:0030541 - plasmid partitioning [Evidence
                     IEA];
                     GO_process: GO:0051290 - protein heterotetramerization
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HRX17087.1"
                     /translation="MSWSIEYHKEADKFLSKNQNISAPLKETLKEFIRFLQGETASIN
                     LKKLSGKWEGYHRIRKGDVRIIFDFDHNEQSIYVRKIDFRGKVY"
     gene            complement(2419..3228)
                     /locus_tag="P5123_12300"
     CDS             complement(2419..3228)
                     /locus_tag="P5123_12300"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRX17088.1"
                     /translation="MSGLYCDAAVARFVTADTVVDGEYSVSGWNRYSYVSNNPVLYKD
                     PTGHYAWSGSTDLDLTKPVDSQLDTKEKNVIFWSYKKEELNAYKISHGKVNWKKFEAE
                     NAFSVAAQTKKDELIINGYNDKNIIIKRLDNIKDLNHNWTKLENNGYNIKSLNIFSHG
                     VFNGPEVAGGSEKFFDKPKKLKYTKDAKVVFHGCHTAGSAKIFAKQQEVETYGQEKYS
                     SFSKSKKLKIPYNLRIKAIKPSKPLYLYDYEKEKASLIHINENGEGVKSNP"
     gene            3733..>3939
                     /locus_tag="P5123_12305"
     CDS             3733..>3939
                     /locus_tag="P5123_12305"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRX17089.1"
                     /translation="MEVTDFRTSIDVEVSRLEKIQEYAEKHNMDISDVMNTVLDKYSK
                     KLAKNPVCNDTLNKYQERGTIYKSV"
BASE COUNT         1365 a          704 c          633 g         1237 t
ORIGIN      
        1 gcctatcact tcttggtgga tgccgtttgt tatcagaaga gggacagcaa cgttttaaag
       61 acgaaaacat gattgagcac tgttacgact ttgtacgatt tgttattgaa catcttgaaa
      121 acgagatcaa agaagataga tgagacaaaa atacataaca aaggtattat ctcttgaatt
      181 gaacaacaag ttatgtaccg gctgcggtat gtgtgcgact gtttgcccgc acaatgtttt
      241 tgatattatg gaaggcaaag cagccgcatc acgcaaacag tattgtatgg aatgcggagc
      301 ctgcaaatta aattgtagat ttaacgcaat aagtgtaaac agcggagtcg gttgcggtta
      361 tgccgttata atgggagcca ttaaaggaac cgaaccagac tgcggatgtt cgagcggatg
      421 ctgttgatat atcagtggat tattttctgt ttgaagatat tagaaaaaaa ctattaagac
      481 aaaccctcga ccaggaaaca gcggatatag tctcccaaat caaccagatc accgatgaaa
      541 aaaggattca ttactcaaca ttttcaagtc tcttgttatg aagaacaaag tcaaagactt
      601 ggtttctatg gtttcgcaga aattccgtat aaaaagcttg tattatttat aacattgtgt
      661 catattaaat atagagtcct ttagtagtga aagccggctc caatctcacg ggtcagcgta
      721 ctcttgccca ctccgccctt tctgattgct gaacgtgatt atttttggca ttgggggggg
      781 tatcctttga aaaaaaattc aaactttcta tatcatatgt gcatatttta tgcgcgtatg
      841 attgatatcc aagcaaaaaa atgttgattt tttgactttt tattccaaat atacaccata
      901 tgacaccgtt aatgctctgg aatacgacac ataggaataa cacaatgact ctcggagaga
      961 aaattaagca cctgcggaaa gaacagaata tctcgcaaac agaactggcc aagattattg
     1021 gcgtgcatca aagccacatc ggtagatatg aacgtgacga gtcaactcca acagcctacg
     1081 ttataaaaaa gcttgcagag gtattgaaag tatcagcaga ctatctatta tctgaagaag
     1141 aggaagaaat tgcagcgtct tttacttccg acaaggaact tttacaacat tttcaggata
     1201 ttagccggct tgccgaccca gacaaacagt ttataaaacg aatgataaaa atggctatta
     1261 atgatgccaa aataaaagag atggccagct aaaagaggta ttatgcaact tgacataaca
     1321 aaagatgagc tttatacaat aataaaatcc gcggtaagag acgtgctaaa agaggataaa
     1381 gttaaacata tacttgattc agccccctat gtttctgatg aagagatgga agacattgtc
     1441 tccattcatg gtgacgcacc tggcccaaaa gaagtttctc gcacagaaat acttgatcta
     1501 tgagctggtc tatagagtat cacaaagagg ctgacaagtt tctctcaaaa aatcaaaata
     1561 tctctgctcc tctgaaagaa acgcttaagg aatttatccg ttttttgcag ggtgaaaccg
     1621 cttcaatcaa cttaaaaaaa ctgtccggaa aatgggaagg ttatcaccga atcagaaaag
     1681 gtgatgtcag aatcattttt gattttgacc ataacgagca gtcaatctat gtcagaaaaa
     1741 ttgacttcag aggaaaagtt tactagaatc taaaaaaccg gaaaccgtca ggtttttcac
     1801 tattgatctc ttataaaaca aaatttcatc agccgactac agatatattc tgtacaccta
     1861 acatagttta agggaacgtt actaagcaat actattaatt actaacatat cagccacaag
     1921 ggggtatact ttgtttaaat atttattagg ctaaggagaa aaacctgaca aaatcacaaa
     1981 ccactaaaga cctttacaaa tgtttcatga ttagaaataa ttcttactat cttccactca
     2041 tttcttataa atttaacctc aatgactcca cttacttctt ctgaatcatt tttatgttga
     2101 aaatacccag ccatactaaa agacatgctg aataaattat ttttttcaac ctcatgaata
     2161 taaatattac attttatatc gtttctagag aaataggaat tacagttatc tagtgcattt
     2221 tgatatgata actgttttcc ttttaaaaca aatacagcat ttttagaaaa taagtcattc
     2281 ttctctaata ttttttttcc tcgatattct ccccaaaatc caaaaaaact tttttcgtat
     2341 taatttccat taaaagcatc atatataaac tcagaaacac acacaaaaga agaaatgaaa
     2401 ttaataaaat tacttttttc atggattact ttttacaccc tctccatttt cgtttatatg
     2461 aataagtgat gctttttctt tttcataatc atataaatac aaaggtttac ttggcttgat
     2521 tgcttttata cgcaaattgt aaggaatttt tagcttttta cttttagaaa aagatgaata
     2581 cttttcttgc ccataagttt ctacttcttg ttgttttgca aaaatttttg cagatcctgc
     2641 tgtatggcaa ccatgaaata caactttagc atctttagta tatttaagct ttttgggctt
     2701 atcaaaaaac ttttcagacc caccagctac ttcaggtcca ttaaatacac catgagaaaa
     2761 aatattaaga gattttatat tgtaaccatt attttctaat tttgtccagt tatgattaag
     2821 gtctttaata ttatccagac gttttataat tatattttta tcattatatc cattaataat
     2881 taattcatct ttcttcgttt gtgcagctac agaaaatgca ttttctgcct caaatttttt
     2941 ccaatttact tttccatgac taatcttgta tgcattcaac tcttcttttt tatacgacca
     3001 aaagattaca tttttttctt ttgtgtccaa ctgactatcc acaggtttag tcaaatcaag
     3061 atcagtactt ccactccacg cataatgacc agtagggtct ttgtatagta caggattatt
     3121 agaaacataa gaataccgat tccaccctga aacagaatac tccccatcaa caaccgtatc
     3181 ggctgttaca aacctagcta ccgctgcgtc gcaatataaa ccagacaaaa tctctggttg
     3241 ttgttttgaa attttattat caaatcaaca ctaaacgtac gctgcgattt tttcaaaagg
     3301 gaaaaattcg ctacgctaat atttgtattg ataatttcaa aaacaattat atgtctggcg
     3361 ttgtaataat agtactaaca atctggcgtt tcaaaaatca aatatctgct ctcaagttat
     3421 gcaaatttac tgctttctgt caaccttttc acgatcttca caaagatgta ttaaatcatg
     3481 tgtaaattcg tttaatgatc aattactgat acccaacgat ggtctttttt gaccatgtaa
     3541 atagggacgg agttatttta acgattatct gaaaatgaat catgtacaaa ctgttacctg
     3601 atataatcca gcaccaatca ttcgtaccaa aaaatatgct gttatccaaa aattacaaat
     3661 aactcaaaaa aattttaaca aagcggtctt caatccagta tacatatata tacatacttc
     3721 ggaggcgata aaatggaagt tacagatttt agaacatcaa tagatgttga agtaagcaga
     3781 ctcgaaaaaa ttcaagagta tgctgagaaa cataatatgg acatctcaga tgtaatgaat
     3841 acagttctgg acaagtattc gaagaaactg gctaagaatc cggtatgcaa cgatactctt
     3901 aataagtatc aagagagagg tacaatttac aaatcggtg
//
LOCUS       DAOTGZ010000246         3938 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_3064
            NODE_27248_length_3938_cov_5.446819, whole genome shotgun sequence.
ACCESSION   DAOTGZ010000246 DAOTGZ010000000
VERSION     DAOTGZ010000246.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868088
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 3938)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3938)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:22:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,785
            CDSs (total)                      :: 2,751
            Genes (coding)                    :: 2,732
            CDSs (with protein)               :: 2,732
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 12 of 19
            Pseudo Genes (internal stop)      :: 5 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3938
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_27248_length_3938_cov_5.446819"
                     /isolate="AMR_MDS_3064"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..181)
                     /locus_tag="P5123_12310"
     CDS             complement(<1..181)
                     /locus_tag="P5123_12310"
                     /inference="COORDINATES: protein motif:HMM:NF039832.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SAP domain-containing protein"
                     /protein_id="HRX17090.1"
                     /translation="MRPELNLKINPQEFLNYYWLKEELSVFCKQNNLPGSGSKDELTN
                     RIYLFLKTGKITEPVR"
     gene            complement(557..1156)
                     /locus_tag="P5123_12315"
     CDS             complement(557..1156)
                     /locus_tag="P5123_12315"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRX17091.1"
                     /translation="MKKFLVIIGFTVVFAIVLCACDDSGDETKEDRCKKAINNSENIL
                     IEAGFSIDSDMSESEKIAACVEEMVDDQIDCYIDADNADELLSCPGVEVEDFIEEDSS
                     CITLDSSISEHVNKNRFDTFVFMPETSGNYTIYLTELDGDCDLMLYSDSLDLLDSSEN
                     SGQESEQITYAMTAHKTYEIDVENWDNMTVSYTITITKD"
     gene            complement(1495..3366)
                     /locus_tag="P5123_12320"
     CDS             complement(1495..3366)
                     /locus_tag="P5123_12320"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:NF019315.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009975 - cyclase activity [Evidence IEA];
                     GO_process: GO:0009966 - regulation of signal transduction
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HRX17092.1"
                     /translation="MLKRIPKTIPEWRKHLSSCFNHNIFLCIFTIVAVLYSVYLFGYF
                     RFNNGARTGDAVSGVLDLVFADFTNDNVYSLGGQWLFAYGAHIAPEEVDSDKYRTLFQ
                     QVTVPASWKNQKYKGRALPGTGIATYRLKIDIGVNVPDELSLLIPGWESAYRLYVNGK
                     LTAWAGIAAEGPENVVSAWSPRVSRFDVNPGINDIVVHISNFSHARGGPGMMPFVGNI
                     SAITNLRERGIALKLFSFGGLLMISLYHIVIFFLRRKERSALWFSLFCLTMALRSMVI
                     NEHGLLIFLPSISWSVHVRITYLVFVLALPVFIMLLRSLFKEEINRCVVATIAGLSAS
                     YALLIVVAPPLVFTEFVLYFQILISVASVYGLVALFQAMRHKREGATAFLIAFLFYLI
                     CIINDILFHRHMVPTGYIVPFGFFSFVFIQALILAKRYTSAFVKMEELFSEKTKLEGA
                     ALTLRNLSYIDQLTSLANRRRLDEYINDGFKRAIRSGDAVSIIMIDVDFFKSFNDQNG
                     HVSGDEVLRLVASTIQSCLRRPADLVARYGGEEFAVFLPSTGIDGARAVAENMRTATL
                     DLRVSAADNIAQRYVTLSLGCASMKPGPDDDPLQLIRKADQALYRAKEKGRNRVETT"
     gene            3637..>3938
                     /locus_tag="P5123_12325"
     CDS             3637..>3938
                     /locus_tag="P5123_12325"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRX17093.1"
                     /translation="MRKIIIAILFPILLLNCSKKSSSEGEEISDLSMEKESAPMTLSA
                     NDLDDSESETRSKKVSSSVKNDEELDPAGYNNFYPVEGRMLEYSVEMHFESENLVS"
BASE COUNT         1315 a          818 c          649 g         1156 t
ORIGIN      
        1 tccttacagg ctcggtaatt ttacctgttt ttaaaaatag gtaaatacga ttggtaagct
       61 cgtctttgct gccgctacca ggcaggttgt tctgtttgca aaaaacagaa agctcttcct
      121 tgagccagta ataattgagg aactcttggg gattgatttt taaatttagc tctggtctca
      181 ttaatcttct ccggtaatta tttaagaatg agttttaaca gcggtttttt tattatgaac
      241 acagggtatt taagcaagtc taaaagcgga ataggcgatt gattgtttcc atattgacta
      301 cttggttcag agacatttta agaattatat gtatacaaag gcattgcgtt gtcatttacg
      361 cttttattgt tgcctttaat taagcaatag tatacaataa ttaatggtgc aaccttcatg
      421 taatgcggta ttgcttataa caagggcata tccctcttcg caaaccgttg agggttgcac
      481 cgttaacatt tatgcctgct acttataatg taaatttact ctttactgct ttaatacatt
      541 tcttattcaa ggctcattaa tctttggtta ttgtaatcgt ataagaaaca gtcatattat
      601 cccaattttc gacatcaatt tcatacgttt tatgagcggt catcgcgtat gttatctgct
      661 ctgattcctg gccgctattc tcggaagaat ctaaaagatc caaggaatca ctatacaaca
      721 taagatcaca atcaccatct aattctgtaa gatagatagt ataattaccg gatgtttccg
      781 gcataaaaac aaaagtatca aaacgatttt tgttaacatg ttccgaaatc gagctatcta
      841 atgtaataca agatgaatct tcttcaataa aatcttctac ttctacaccg ggacaactta
      901 gcaactcgtc tgcattatca gcatcgatgt agcaatctat ctgatcatct accatttctt
      961 ctacacaggc agcaatcttt tcactttcac tcatgtcaga atctattgaa aatccagctt
     1021 ctattaaaat gttttctgaa ttatttatag cctttttgca acgatcctcc tttgtttcat
     1081 cccctgaatc atcacaagca catagcacaa tagcaaaaac aacagtaaaa ccaattataa
     1141 ctaaaaattt tttcatactc tttttccttg aatttattat gtaaactttt taaaaaatca
     1201 ctggttcatc tattttgcgt ctaaattggt tatcttacaa attataatct ttaaaaaact
     1261 aaatgcttta attaataata gcgaagcctt ttgcaagaaa ttataaacag tataaatcct
     1321 gacaaaaata tgactgtcaa gcaaaagggg aacaaaaatc agccagaaac aaaaaatttt
     1381 agtattttac gtttagctaa tgtgtacaat cgccagtata aggattctaa aacaagactt
     1441 atattataca gctctgtgtc gaaaaattag ttaattctga cgagctctaa attgtcaggt
     1501 cgtctctacc cggtttcgcc ccttctcttt tgcacgatac agcgcctgat cggcctttcg
     1561 aataagctgc aaaggatcat catcgggacc aggtttcata gaggcgcacc ccagacttaa
     1621 tgtaacatat cgttgagcaa tattatcggc agctgacaca cgaagatcaa gcgtagctgt
     1681 acgcatattc tctgccacag cccttgctcc atcaatcccg gtggaaggca aaaatactgc
     1741 aaactcttct ccaccgtatc gggcaaccag atcagctggg cgtcgcaagc acgactgtat
     1801 tgtcgaagca acaagacgca gtacctcatc accagagaca tgtccatttt gatcgttaaa
     1861 cgatttaaaa aagtcaacat caatcatgat aattgaaaca gcgtctccag accttatggc
     1921 acgtttgaaa ccatcattaa tatattcatc aagccgtctt ctatttgcaa gacttgttaa
     1981 ctgatcaata tatgaaagat ttcgcaaagt taaagcagca ccctcaagtt ttgttttttc
     2041 agaaaatagc tcttccattt taacaaaggc agaggtgtac cttttggcca aaattagagc
     2101 ctgtataaaa acaaatgaaa aaaaaccgaa gggtacaata taacctgttg gcaccatatg
     2161 acgatgaaaa agaatatcat taataataca tatcaaataa aataaaaaag ctattaaaaa
     2221 agcagtggcc ccctctcttt tatgacgcat agcttgaaaa agagcgacca gtccgtaaac
     2281 tgaagccacg gatatcaaaa tctggaaata gagcacgaat tccgtaaaaa caagaggagg
     2341 agcaactaca atcagcaaag catatgatgc agaaagcccc gcaatcgttg caacaacaca
     2401 acggtttatc tcttccttaa atagagatct tagcaacata ataaaaacag gaagcgccaa
     2461 cacaaaaacc agataggtta tacgcacatg gacactccag gatattgacg gaaggaatat
     2521 cagaagtcca tgttcattga ttaccatgga ccgtaaagcc atagttaagc aaaacagcga
     2581 aaaccagaga gcgcttcgct cttttcttct taggaaaaag attactatat gataaagcga
     2641 tatcataaga agaccgccaa agctaaaaag cttcagtgca attccccttt ctcgcaaatt
     2701 ggttattgcg gatatatttc caacaaatgg catcatccct gggccacccc tggcatgact
     2761 gaaatttgaa atatgtacta caatatcgtt tataccggga ttaacatcaa aacgtgaaac
     2821 gcgcggagac caagccgaca caacattttc aggaccttca gccgcaattc cggcccaagc
     2881 agtcaacttg ccattaacat atagcctgta agctgattcc caaccgggaa tcagcaagga
     2941 aagttcatct ggcacgttca ccccgatatc gattttaaga cggtaagtcg ctattcccgt
     3001 tcctggcaat gctctgccct tatatttttg atttttccat gaagcgggaa cagtaacctg
     3061 ttgaaaaagg gttctatact tatcactatc cacttcttcg ggcgctatat gagcaccgta
     3121 cgcaaataac cactgccccc caagagaata gacattatca tttgtaaaat ccgcgaaaac
     3181 tagatcaagc accccgctaa cagcgtcacc cgttcgagct ccattattga aacgaaaata
     3241 tccaaacaaa taaacggagt acaaaacagc aactattgta aaaatgcaaa gaaaaatatt
     3301 atggttaaaa catgaggata aatgtttgcg ccattcagga atggtctttg gtattctttt
     3361 tagcatcatg gcacccgact tatcatttaa aaaaacgtac cattttataa ctataaatca
     3421 acttattttt ttctttcagg acttttgata ttggcataaa aggctgaaaa tatgacattt
     3481 tttataatat atcataatca tcatttctta taatcatcat tcctcaacag ctgacttact
     3541 tatcacaaag caaatagctt gacattttta aaacagctcg tctaatcaag cattactctg
     3601 gttttctttt tgttaattaa ccattttgga ggcaatatga gaaaaattat catcgcaatt
     3661 ctgtttccca ttctattact caactgctca aaaaaaagct cttctgaggg ggaagaaatc
     3721 tccgatttat ctatggaaaa agaatctgcc ccaatgactt tatccgccaa tgatttagac
     3781 gattccgaat cagaaaccag atccaaaaaa gtctcttcat ctgttaaaaa cgacgaggag
     3841 cttgacccgg cgggttataa caatttttac ccagtagagg gtcgaatgct tgagtactca
     3901 gtagagatgc acttcgaaag cgaaaatctt gtctctag
//
LOCUS       DAOTGZ010000247         3930 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_3064
            NODE_27318_length_3930_cov_4.927484, whole genome shotgun sequence.
ACCESSION   DAOTGZ010000247 DAOTGZ010000000
VERSION     DAOTGZ010000247.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868088
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 3930)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3930)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:22:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,785
            CDSs (total)                      :: 2,751
            Genes (coding)                    :: 2,732
            CDSs (with protein)               :: 2,732
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 12 of 19
            Pseudo Genes (internal stop)      :: 5 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3930
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_27318_length_3930_cov_4.927484"
                     /isolate="AMR_MDS_3064"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            500..1930
                     /gene="gatB"
                     /locus_tag="P5123_12330"
     CDS             500..1930
                     /gene="gatB"
                     /locus_tag="P5123_12330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013008630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016884 - carbon-nitrogen ligase activity,
                     with glutamine as amido-N-donor [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatB"
                     /protein_id="HRX17094.1"
                     /translation="MELEAVIGLEVHAQLNTKTKIFCGCPTSFGSESNTQICPVCTGM
                     PGVLPVLNREVLHKSILAGLALNCKISEYSVFDRKNYFYPDLPKAYQISQFDKPICIG
                     GVVPIVADGVKRDIQLTRIHMEEDAGKNIHSDDISNPVSYVDYNRTGVPLMEIVSEPD
                     MHTGEEAYQYLSNLRKILRYINVSDCNMEEGSFRCDVNISLRPKGETKLGEKVEVKNM
                     NSFKAVKAAIEYEIKRQTIMIEDKERIKQETRLWNPDKSITESMRSKEEAHDYRYFPD
                     PDLAPITVTQDQIKKIREELPELPVQKLERFKKEYALPDLDAETLCASRSLADYFESV
                     VKSGHDAKKAANWIANELLSFVENIESMDSFKVTPEMMSEMLSLIDKSVISGKIAKKV
                     LSLMIETGKTASRIVEDEGLKQVTDTGAIEAMVDDVLKANPEIVEEIRNGKDKAKGFL
                     VGQIMKAAKGKANPQLVNEILAKKLS"
     gene            complement(1988..3721)
                     /locus_tag="P5123_12335"
     CDS             complement(1988..3721)
                     /locus_tag="P5123_12335"
                     /inference="COORDINATES: protein motif:HMM:NF024140.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein"
                     /protein_id="HRX17095.1"
                     /translation="MKWFSNLKVASKVLLSCLILIIFTVAISVSSLMLLNQADDHFQS
                     FYADRFIPVRELNRMAKNVLQMRINMMAELVAYENNDAAEMDRRMKSSAELAEQNAKI
                     WEEYLKGNIAGDEEVLKNKIEDLEKRRTLVRAEFGRALHNNDLELAAEKSSEWVTIYR
                     ESKTAMDNMMQFQQDNANKLREDMDASSRFALLFNIAMLTISVFLGIIITIVLARAIS
                     RPVAKGLKFAQDLANGDFRNRIDLDQNDELGQLGKALNTAADDLENTMTEIIVGVQNL
                     VQAITEISAGNENLSQRTSEQASSLEEIASTIEENTATINKNADNSIEANKLSKNTSV
                     LAEDGGTVMDEALSSINDINESSKKIEEIISVINEIAFQTNLLALNAAVEAARAGEQG
                     RGFAVVAGEVRNLAQRSGNASKEIATLIQETLGRVGKGTELTNKAGESLREIISSIND
                     VSRFISEIAVASEEQKRGTEQINMAVSELDSMTQQNAGLVEETASASEEMSNQAQELL
                     QKVEKFKVRESKTQSAVQNRAIHIKSLEKKPAAYRQESQQKPNQVQRNGNVKKQDDIE
                     KILTNDGFESF"
BASE COUNT         1183 a          804 c          738 g         1205 t
ORIGIN      
        1 gtatagtttg ctgtgtttgg taactgcagc agattgtaaa tatataaaaa gagcataacc
       61 cgccagcata gtaattaata aaatcactga tagtataagg gcaaatctag tttgactgct
      121 ttttataaaa gataatacgc gttcagctgt cataaataaa tcaccaacta gtcagatata
      181 ttttcaaatc tttataatct attcgtaaca atttatatgg caacaaaata attttcacca
      241 aatcataatg atttttactt ttttatataa ttgcgtctct ttcaaatctg aataactatg
      301 atatttctaa ttatttctca aatacttaaa atattaatcc attcaaatat caccatgatt
      361 gatagcaagc acttttactt gacaaataga cctctgatct tagggtaaca gacgattatc
      421 tgatcttatc ggtttgatgg tttatcaggc taaatataga gctatatctt tttatcataa
      481 tatgatgccg gaggcttaaa tggagttgga agcagttatc ggactggaag tacacgccca
      541 gcttaatacc aaaacaaaaa ttttttgcgg ttgcccaaca agttttggtt cagaatccaa
      601 tacccagata tgtccggttt gtaccggcat gcccggcgtt ttacctgtac taaaccgtga
      661 ggttctgcat aaatccatac ttgccggatt agccctgaat tgtaaaatat cagaatattc
      721 tgtttttgac agaaaaaact acttttaccc tgatcttcca aaggcatacc agatttcaca
      781 gtttgataaa ccgatatgta tcggtggagt tgtacccata gttgctgacg gagttaaacg
      841 tgatattcaa ctaacccgta tccatatgga agaagatgct gggaaaaaca tccattctga
      901 cgatatttcc aacccggtat cttatgttga ctataaccga acaggtgttc cattgatgga
      961 gattgtatcc gaaccggata tgcacaccgg tgaagaagct tatcagtatt tatcaaatct
     1021 tagaaaaata ttacgctata taaatgtttc tgattgcaat atggaagagg gatcttttcg
     1081 ttgtgatgtt aatatttcac ttagaccgaa aggtgaaaca aaacttggtg aaaaagtaga
     1141 agtaaaaaac atgaactcct ttaaggctgt taaggcggct attgaatacg aaattaagcg
     1201 tcagaccata atgattgaag ataaggagcg cattaaacag gagacgaggc tctggaatcc
     1261 tgacaagagt attaccgaaa gcatgcgcag caaggaagag gcgcatgact accgctactt
     1321 ccctgacccg gatcttgcgc ctataactgt gacacaggat caaatcaaaa aaatcaggga
     1381 agaactacca gaattgccgg ttcaaaaact cgaaagattt aaaaaggagt acgcactacc
     1441 cgatcttgac gcggaaaccc tctgtgcttc tcgctcactt gccgattatt ttgagtctgt
     1501 tgtaaaatcg gggcatgacg ctaaaaaagc tgccaactgg attgcaaatg agctactctc
     1561 atttgtagaa aacattgagt caatggacag tttcaaggtt accccagaaa tgatgtcaga
     1621 gatgctatca ctgattgata aatctgttat cagcggtaaa attgcaaaaa aagtactttc
     1681 actaatgatc gaaaccggaa aaacagcttc tcgcattgtt gaagatgagg gtcttaaaca
     1741 ggttacagat accggcgcta ttgaggcaat ggttgacgat gttctaaagg ctaaccccga
     1801 aattgtagag gaaatacgaa acggaaagga taaggccaaa ggatttcttg taggtcagat
     1861 aatgaaagca gctaaaggaa aagccaaccc tcagttagtt aacgagatac tcgccaaaaa
     1921 gctgagttaa tacataaaaa tccctgctat ttaacaggga tttttactag aatattttta
     1981 agcataatta gaatgactcg aaaccatcgt ttgttaatat tttctcgata tcatcctgtt
     2041 ttttaacatt tccgtttcgt tgaacctgat taggcttttg ttgactctcc tgtcgataag
     2101 ctgcaggttt tttttctaac gattttatat gtattgccct gttttgaacc gcagattgcg
     2161 tcttggattc acgaaccttg aacttttcaa ccttctgtaa cagctcctgt gcctgatttg
     2221 acatctcttc ggatgcggat gctgtctctt cgacaagacc tgcattttgc tgtgtcatac
     2281 tatcaagttc gctaactgcc atatttatct gttcagtacc gcgcttctgc tcttcggatg
     2341 caacggcaat ttcggaaata aaccgactaa catcatttat cgaacttatt atttcgcgta
     2401 gcgattcacc agccttgttg gttaattcgg ttcccttgcc aacacgcccc aatgtttcct
     2461 ggatcaatgt agcaatctct tttgacgcat tgcctgaacg ctgtgcaaga ttacgtactt
     2521 caccggcaac aaccgcgaat ccacgcccct gctcgccggc tcgagcagct tcaacggcag
     2581 cattaagagc aagaaggttt gtctgaaagg ctatctcgtt aataacagag attatctctt
     2641 cgatcttttt acttgattcg tttatatcgt ttatggatga gagagcttcg tccataacgg
     2701 ttccgccgtc ctctgccaaa acagatgtgt ttttggaaag cttatttgct tcgattgagt
     2761 tatcggcatt tttattaata gtggccgtat tctcttcaat tgtggaggcg atctcttcaa
     2821 gagatgacgc ctgctctgat gtgcgttgag acaggttttc gtttccagcc gaaatttcag
     2881 ttatggcctg aacaagattt tgtaccccca ctatgatttc ggtcatagtg ttttcaagat
     2941 cgtcagcagc agtgttaaga gctttgccaa gctgtccaag ctcgtcgttc tgatcaagat
     3001 cgattctatt tctaaagtct ccgttagcaa gatcctgcgc gaattttaac cctttagcaa
     3061 ctggtctaga tattgccctt gccaatacta ttgtgataat aattcccaga aataccgata
     3121 tagttaacat tgcaatatta aaaagaagtg caaaccgcga agaagcatcc atatcttcac
     3181 gaagtttgtt ggcattatcc tgctgaaact gcatcatatt atccatggca gttttgcttt
     3241 cgcgataaat ggttacccac tcactgctct tctctgcggc cagctcaaga tcattattat
     3301 gcaaggctct tccaaactct gcgcgcacaa gggtacgtct cttctcgaga tcctctattt
     3361 tgtttttgag aacctcctcg tcaccggcta tattaccttt taagtattct tcccaaattt
     3421 tagcgttctg ttctgccagt tcggcgctac ttttcatgcg acgatccatc tcggcagcat
     3481 cgttattctc ataagccacc agctcggcca tcatgttaat acgcatctga agcacgtttt
     3541 ttgccatccg gttaagctca cgtaccggta taaaacgatc cgcgtaaaac gattgaaaat
     3601 gatcatcagc ctgattcaga agcatcaatg atgataccga aattgctact gtaaatatta
     3661 ttaaaataag acaagataaa agaactttgc tagcgacttt aagattactg aaccatttca
     3721 tagcactcca cctgtaatta tacaaagatg ttaataataa ctatatgcta atacaaaaca
     3781 gttacagtat caaaatatta aatcatactt tttatcggta tggtttccat accatgatta
     3841 ttagataaat ttgaagaaag ttaatcgata ttaataccgc ggttaataac ccattttact
     3901 gccgagcggc ttaatagtgc ggcattgtct
//
LOCUS       DAOTGZ010000248         3904 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_3064
            NODE_27547_length_3904_cov_4.891400, whole genome shotgun sequence.
ACCESSION   DAOTGZ010000248 DAOTGZ010000000
VERSION     DAOTGZ010000248.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868088
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 3904)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3904)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:22:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,785
            CDSs (total)                      :: 2,751
            Genes (coding)                    :: 2,732
            CDSs (with protein)               :: 2,732
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 12 of 19
            Pseudo Genes (internal stop)      :: 5 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3904
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_27547_length_3904_cov_4.891400"
                     /isolate="AMR_MDS_3064"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1518)
                     /locus_tag="P5123_12340"
     CDS             complement(<1..1518)
                     /locus_tag="P5123_12340"
                     /inference="COORDINATES: protein motif:HMM:NF014647.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="BMP family ABC transporter substrate-binding
                     protein"
                     /protein_id="HRX17096.1"
                     /translation="MYNETTVEIYRQAQKQAKTNFLKRVSDGGSGYLPALDGLIRHMD
                     IASEINIGTHEIPLKKIIGTASHSRATAFARNFYPIQQLNTEFASKWMMLYDAHLSDG
                     IRDSITAYEYLNWFYVREGNKRVSVLKYNNAYSINAEIRRIIPKRDPSDMTNTIYYEF
                     LEFNKLTQIFTIWFSRPGRFPKLIQHLSGYNPKLLLNVNKYQKFFSNIYMPFSKIYHE
                     LDGDKLDITTADALLQFLDLYGIPDEITEREHRTTIKKLFPEFETLNSSVATDLTSPQ
                     KRSASNVINAIAKKVTNKKLKIGFINAKSPETSGWTNSHEQGRQHIISMLGKMVETTY
                     INDVPQDENAYDYIDKLAAKKMDVIFTTSPTFKNTTIKAALEHPKIKFFNCSAEDSSK
                     NVRSFFGRIHEVRFLMGLIAGSLTKTDQIGYVASYPIPEVISSINAFALGAAMVNPLA
                     RVHVRWMYRWENESLSFQGAHELSEMGIDLISQDDLPILKSKKKAYGLFSTKRDEK"
     gene            1821..3623
                     /gene="typA"
                     /locus_tag="P5123_12345"
     CDS             1821..3623
                     /gene="typA"
                     /locus_tag="P5123_12345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012861919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA];
                     GO_process: GO:0006950 - response to stress [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translational GTPase TypA"
                     /protein_id="HRX17097.1"
                     /translation="MKKIRNVAIIAHVDHGKTTLIDALLRQSGLYRDNQTLDERVMDS
                     NDIEKERGITIFSKNASIPYDDVKINIVDTPGHADFGGEVQRIIKMVDSVLLVVDAFE
                     GPMPQTRYVLKKALSEGHCPIVVINKIDRPDAQPESVVDKVFDLFVELEAEDNQLDFP
                     VIFASAKEGLAHKTLYDESSDMTPLFQAIIEHVKEPEGDDAQPFQFLVSAIDYDNYIG
                     RLCTGKIANGVLRAGQEVCHIDSEDVKKKYKVTRLMAYQGLQRVEIDEAFAGDVITIA
                     GIDEISLGDTLCDVDNPKKLESITIDEPTIAMTFSVNDSPFMGREGDFVSSRHLTERL
                     NRELKNNVSMKVEKSHLKDSYVVKGRGELQLSILIENMRREGYEFQVSRPQVIMQEIE
                     GKKCEPIEHAIIDVADDFTGVVIEKMGLRKAELLGMTQGTDGYSRLEFKVPARGLIGF
                     RNEFLTETKGTGILNHSFYGYEPFKGALASRNRGVLVAMEAGVSAAYAIFNLQDRGIF
                     FVDSGTEVYEGMIVGEHNRDNDLVVNVCKTKKLTNMRASGSDENVRVAPPRKFSLEQA
                     LEYIQDDELLEITPQNVRLRKKILDENSRRKASR"
BASE COUNT         1132 a          676 c          911 g         1185 t
ORIGIN      
        1 cttctcgtct ctttttgtgg agaacagacc ataagctttc tttttacttt tcagaattgg
       61 aagatcgtct tggcttataa ggtctatacc catttctgat aattcgtgtg caccctgaaa
      121 agagagtgac tcgttttccc agcgatacat ccacctgacg tgaactcggg caagcggatt
      181 aaccatggcc gcaccaagcg caaaggcgtt tatactgctt atgacttccg gtatcggata
      241 cgaggcaaca tatcctatct gatctgtttt ggtaagtgaa ccagctataa gccccattaa
      301 gaagcggact tcatgtatgc gtccgaagaa agagcgtacg ttttttgatg aatcttcagc
      361 agagcagttg aagaacttga ttttggggtg ttctaacgct gccttgatag ttgtgttttt
      421 aaaagtcgga gaagttgtaa agattacatc catctttttt gcagcaagtt tatcgatgta
      481 atcgtaagca ttttcgtctt gcggcacgtc atttatatat gttgtttcga ccattttgcc
      541 aagcatgctt ataatatgct ggcgcccctg ttcgtgtgag ttggtccagc ctgaggtttc
      601 gggagatttg gcgttgataa agcctatttt cagtttttta ttggtaactt tttttgcaat
      661 ggcgttgata acattggatg cagacctttt ttgaggtgat gtcaagtcgg tcgccacact
      721 gctgtttaag gtttcaaact cgggaaacaa tttcttgatt gtggtacggt gctctctttc
      781 tgttatttcg tctggtatgc cgtacagatc aagaaactga agcagtgcat cggcagttgt
      841 tatgtccagc ttgtctccgt caagttcgtg atagatcttt gaaaagggca tatatatatt
      901 ggagaaaaat ttttgatatt tattaacgtt taataataat ttgggattgt atcccgacaa
      961 gtgttggata agttttggaa agcgacccgg acgtgaaaac cagatcgtga atatttgagt
     1021 caatttgtta aattccagaa attcataata tattgtattt gtcatatccg aaggatcgcg
     1081 tttggggatg attcttcgaa tctctgcatt gatggagtag gcgttgttat actttaatac
     1141 ggaaaccctc ttgttacctt ctctgacata aaaccagttg aggtattcgt atgcggtaat
     1201 gctgtcacga atgccatccg aaagatgggc atcgtagagc atcatccact tgctagcaaa
     1261 ttcggtgtta agttgttgta tcgggtaaaa attacgggcg aacgcggttg cacgcgaatg
     1321 agatgctgta cctatgattt tttttagagg tatttcgtgt gtgcctatat tgatttcaga
     1381 ggcgatatcc atgtgtcgta tcagtccgtc aagtgctgga agatatcctg aaccaccatc
     1441 agataccctt ttcaaaaaat ttgttttggc ctgcttttgt gcctgacggt aaatctctac
     1501 tgttgtttca ttgtacatta tgactcctgc gggcagtcct gcaataggtg aaacaccgga
     1561 tttttgcaag gtttatgaac tatatatagt ggattaaaga aaacaggtta attgtcaaca
     1621 cttttagaaa ctgaagatta tttcatcggt tttgaatcct gcattttaac ataatatatc
     1681 tttttttatt tacataaatc attataaaaa tacattgtca tctcttatta tgcgttttaa
     1741 gtgcattccg ctggaacttc ccgcattttc agaagctacc tcgcaggttt agccgaatag
     1801 tcaaggatat agataaacaa atgaagaaga ttagaaatgt cgcaatcatc gcccatgtag
     1861 atcatggtaa aacaacatta attgatgccc ttttgcgcca atcgggattg tatcgagata
     1921 atcagactct cgacgaacgg gtcatggact ccaatgatat cgaaaaagaa cggggaatta
     1981 ccatattctc caaaaatgct tccattcctt atgatgatgt gaagataaac atagtggata
     2041 cacccgggca cgcggatttt ggcggagaag tacagcggat cataaaaatg gtcgattcgg
     2101 tattgctggt tgttgatgcg tttgaaggac caatgcctca aacaagatac gtgttaaaga
     2161 aggccctttc tgaaggccac tgcccgatag tggttattaa taaaatagac cgccccgatg
     2221 ctcagcctga aagtgttgtt gataaggtgt ttgatctttt tgtagagctt gaagcagagg
     2281 ataatcaact cgatttcccg gtaatttttg cttcggccaa agaaggattg gcgcataaaa
     2341 cgctgtatga cgagagcagt gatatgactc cgctatttca agcaataata gagcatgtta
     2401 aagagccgga aggtgatgac gcacagccat tccagtttct tgtctcggca attgattatg
     2461 ataactatat aggaaggctt tgcacgggta agattgcgaa cggagttttg cgtgctgggc
     2521 aagaagtttg ccatattgac tctgaggatg ttaagaaaaa atataaagtt acaagactga
     2581 tggcgtatca ggggctgcag cgtgtcgaga tagatgaggc cttcgccgga gatgtgataa
     2641 cgatcgcagg tattgatgaa atctcgctgg gagataccct ttgtgatgtc gataacccca
     2701 aaaaactgga atcaataacg attgatgaac ctacgattgc catgacgttc tctgtgaatg
     2761 attctccatt tatggggcgt gagggtgatt tcgtgagttc gcgacattta accgaacgtt
     2821 taaaccgcga gctcaaaaat aatgtgagca tgaaggttga aaagtctcat cttaaagatt
     2881 cttatgtagt taagggacgt ggtgagcttc agctatcaat tcttattgaa aacatgcgcc
     2941 gggaagggta tgagttccag gtttcaaggc cacaggtaat tatgcaagaa atcgagggca
     3001 aaaaatgtga accgatagaa catgctatta ttgatgttgc tgatgacttt accggggtag
     3061 ttatcgagaa gatggggctg cgaaaggcag agctattggg aatgactcag ggcactgatg
     3121 gatatagcag gcttgaattc aaggttccag ctaggggatt gatcggtttt cgaaacgagt
     3181 ttttaaccga aactaaggga acgggtattt tgaaccattc gttttatggg tatgaaccct
     3241 ttaagggggc tttggcttcg cgtaacaggg gagttcttgt ggcaatggaa gccggagtat
     3301 cagcggcata cgcgattttt aacctgcagg accgcggtat attttttgtc gattcaggta
     3361 ccgaggttta tgagggaatg atcgttggag aacataaccg tgataacgat cttgttgtaa
     3421 atgtttgtaa aacaaaaaaa cttacaaaca tgcgtgcttc ggggtcagac gagaatgttc
     3481 gtgttgcacc accacgtaaa ttttctttgg aacaggcact cgaatatatt caggatgatg
     3541 aacttcttga gataacaccg caaaacgtca gattaagaaa aaaaattctt gatgaaaaca
     3601 gtcgaagaaa agcatccaga taaacaagga aacagcagat acaaaaataa taacttgtag
     3661 attgttgcaa agtatagaat tgttttttta ttcagttaaa gaggccggtg attaccggtc
     3721 tcttttgata taatgtaaaa aaagtaattg caagttaatg atgaaatggc gaaaactagt
     3781 ttaaaaagcg aaacattggc aaaagatgca ttgaattagg gaaatttcaa ataaccagtt
     3841 tatagtattt gtatgcaaaa aatgtcaaaa gagtctgact tggttatggc tgccatgttt
     3901 ttat
//
LOCUS       DAOTGZ010000249         3861 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_3064
            NODE_27944_length_3861_cov_4.964004, whole genome shotgun sequence.
ACCESSION   DAOTGZ010000249 DAOTGZ010000000
VERSION     DAOTGZ010000249.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868088
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 3861)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3861)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:22:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,785
            CDSs (total)                      :: 2,751
            Genes (coding)                    :: 2,732
            CDSs (with protein)               :: 2,732
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 12 of 19
            Pseudo Genes (internal stop)      :: 5 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3861
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_27944_length_3861_cov_4.964004"
                     /isolate="AMR_MDS_3064"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..801)
                     /locus_tag="P5123_12350"
     CDS             complement(<1..801)
                     /locus_tag="P5123_12350"
                     /inference="COORDINATES: protein motif:HMM:NF015338.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease/exonuclease/phosphatase family
                     protein"
                     /protein_id="HRX17098.1"
                     /translation="MKLKKIATITLSILPLFVFILFITIKLTTYQPKDIEPAPINNQN
                     HAPLLADNSKIKILSWNLQYMAGKKYLFFYDEWDGSGPDKRPSPEEIQKTFKRVVQII
                     NAEDPDILLLQEIDVDSKKTDYDDQLAILLSMISPEYKSYSSCWYWRASFVPHPKIMG
                     KVGLKLAVISKYKIISSERHQLPVMPGNFVIRNLNFKRAILKTEIGLKSGQTLTVMST
                     HLDAFAQGSDTMQKQVQTVSDLLNNLNYNSKNWLIAGDFNLIPPGQSYS"
     gene            1172..2740
                     /locus_tag="P5123_12355"
     CDS             1172..2740
                     /locus_tag="P5123_12355"
                     /inference="COORDINATES: protein motif:HMM:NF037997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005436 - sodium:phosphate symporter
                     activity [Evidence IEA];
                     GO_function: GO:0015114 - phosphate ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0035435 - phosphate ion transmembrane
                     transport [Evidence IEA];
                     GO_process: GO:0044341 - sodium-dependent phosphate
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Na/Pi symporter"
                     /protein_id="HRX17099.1"
                     /translation="MSLADVAVIAGGAGLFLLGMNMMTEGLKAFAGDNLKNLLARFTR
                     GRFSSVALGAVVTTMVQSSHVTTIAAIGFVGAGMISFHNSLGIIFGANLGTTTIGWIV
                     SLLGFKLKIAVISLPIITVGAMMRLLSNDRLNYIGTVFAGFGLIFVGIDFLQTGMTSL
                     VSYIDLGYFNGYTVFGRFMLVIVGVIMTLIMQSSGAAITMTLAALFSSGITIEQGAAL
                     VIGQNIGTTLTAALAGIGGTVQSKRTALAHVLFNVLSAGIAFVILPVFLSIIEYMLKA
                     VKMDEPVYQLSAFHTSFNILGVMIFLPFIKSFASLVEKIIPESKGKFKIKLDDSISSI
                     PSLALGTVKLSLMECLIILLYGLREIITSEHKTSTSQADITDVETMLAEVKKYMSRIR
                     TGRKSSEDAYQLHINLLHALEHLEFIVSAALEKSATKLAKTDETNELIKTFDYIIDTM
                     ENRSANIDVFYLEESAKKINANRYKRRHKILTRTAYGELSPENAYSEIESVRWLDRVS
                     YRVWKSVYYLTIAD"
     gene            complement(2775..>3861)
                     /locus_tag="P5123_12360"
     CDS             complement(2775..>3861)
                     /locus_tag="P5123_12360"
                     /inference="COORDINATES: protein motif:HMM:NF024742.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HRX17100.1"
                     /translation="RLFLIISIIPFTLSTALIFFPPIAGPSVINIIYLSLMFSLFFLF
                     YTMYVAPYIALIPELAHTEEKRLSITTAQGYFALIGGAIAMIGGSALIGVFRKKYTLT
                     KSYQFTVIVLCIIGFIFLLMAILAVNEKKYTKSVPSSLAIIPSIKKTLSNSYFIIFLL
                     SYMSGWFVFNILRSSAVHIGETMMGLNVEESGLNFIILFVSAGLSFVGVMFFSKKYGK
                     KNVMITGLLLFSIFSIGLALTGILPFDKNLYGKVFWGLLGIPTAVFLILPNVFIAELC
                     DYDYKLTGENREGIYFGVNGLFLKLNLGLSTAAASVLYVFFGKDISNPLGIRLSLLLG
                     AAVSIIGTIVLMKYPNKLSEKAIQKAA"
BASE COUNT         1234 a          615 c          738 g         1274 t
ORIGIN      
        1 gctgtaagac tgtccgggag gtatcagatt aaagtctccc gcgataagcc agtttttaga
       61 attataattc aaattattta gcagatctga aactgtttga acctgttttt gcatggtatc
      121 acttccttgc gcaaaggcat caaggtgtgt actcattaca gtcaaagttt gtccggattt
      181 tagccctatt tctgttttta atatggcgcg tttaaaattc aggttacgta taacaaaatt
      241 tccaggcata actggtaatt gatggcgctc agatgaaata atcttatatt tcgaaatcac
      301 ggcaagttta aggccaactt tgcccatgat tttggggtgt ggcacaaacg atgcgcgcca
      361 ataccagcaa gagctgtatg atttgtattc gggtgaaatc attgataata aaatagcgag
      421 ttggtcatcg taatctgttt ttttcgaatc tacatcaatc tcctgtagca aaagaatatc
      481 cggatcttca gcattgatta tttgaacaac acgtttaaat gttttttgta tctcttcagg
      541 cgaaggtctt ttgtcgggac cagacccgtc ccattcatca taaaaaaaca gatatttttt
      601 tccggccatg tattgaagat tccaagataa aatttttatt ttggaattat ccgcaagaag
      661 gggggcgtga ttttggttgt tgataggtgc aggttcaata tctttgggct ggtatgttgt
      721 cagttttatt gtaataaata aaataaatac aaacaggggt agaatagaaa gagtaattgt
      781 tgcaattttt tttaatttca ttttatacca ttataaaaat ctgattgtat ttgttttaaa
      841 acattatgat gatttgtcaa gtattcgaat ataagttgat aaatcatgag tttgagtttg
      901 aatataaaga aagttaaaaa agataatttt tttagttgtc tacaatatta agatataatc
      961 tcgtaattac tgtggttatg cattttagat ttacagctat atttaaatct gtattcagct
     1021 atgtgattat tcgttttata tgacaataat ttacaaacgg ctagaaaatt gtcatatata
     1081 tagttgaaat catttaaaat gtttatattg aacaaaaagt tgaaaatcaa tatttcacta
     1141 ttgatacatt cagaacaaac aggatggttt tatgagtctt gccgatgttg ctgtgattgc
     1201 cggtggagcc ggtctatttt tacttggtat gaatatgatg accgaagggc taaaagcctt
     1261 tgcaggggat aacttaaaaa atcttctcgc gagatttacc agagggcgct tctcttcggt
     1321 tgctttaggg gctgttgtaa ctacaatggt tcaatcttca catgtaacta caatagctgc
     1381 tataggtttt gtaggtgctg gcatgatttc gttccataat tctcttggaa taatttttgg
     1441 agctaatctt ggtactacaa caatcggctg gatagtatct cttttggggt tcaaactaaa
     1501 aatcgcggtt atttctcttc cgataatcac tgtgggcgcg atgatgcgcc ttttatctaa
     1561 tgatcgtctg aactatatag gaactgtttt tgcgggtttt ggtctgattt ttgtaggaat
     1621 cgacttttta caaactggga tgacttcgct tgtttcgtat atagacctcg gttactttaa
     1681 tggatataca gtttttggtc gttttatgct tgttatagtc ggtgttataa tgaccttgat
     1741 tatgcaatca tcaggagctg caattaccat gacacttgct gcactttttt catctggaat
     1801 tacaatagag cagggggctg ctctggtaat tgggcagaat attggaacaa ctctgacagc
     1861 tgccctagcg ggtataggtg gaactgttca atccaaaaga acagccttag cccatgtgct
     1921 tttcaatgtg ttatcggcag gtattgcttt tgttatcttg ccggtttttt tatctataat
     1981 tgagtacatg ttaaaagctg ttaagatgga cgaacctgtt tatcaattgt cggcttttca
     2041 cacttctttt aacatcttag gagtaatgat atttttacct tttattaaaa gttttgctag
     2101 tttagttgag aaaattattc ctgagagtaa gggaaaattt aaaataaaac ttgatgatag
     2161 tatcagttcc ataccatctt tggctctagg aaccgtcaaa ttaagcctta tggagtgtct
     2221 aataatactt ctatatggtt tacgtgagat aattacaagt gaacataaaa ccagcacaag
     2281 ccaggctgat ataactgatg tagaaacaat gttggcagag gtaaaaaaat atatgtctcg
     2341 aattcgtaca ggacgtaaaa gcagtgaaga tgcatatcaa ttacatataa atttacttca
     2401 tgcacttgaa catctagaat ttattgtttc agctgcctta gaaaaatcag caacaaagct
     2461 tgctaaaacc gatgaaacga atgaactgat aaaaaccttt gattacatta tagacacaat
     2521 ggaaaataga tccgccaata ttgatgtctt ttatcttgaa gagtcggcta aaaagattaa
     2581 cgcaaatcgt tacaaaagac gtcataaaat tttaactaga acagcttacg gtgagctgag
     2641 ccctgaaaac gcctattcgg aaattgaatc ggttcgttgg ttagaccgtg tttcttatag
     2701 agtatggaag agtgtctatt acttaaccat tgctgactag gtaatagacg ttttacgctt
     2761 aattgatgta aaaattacgc tgctttttga atagcctttt ctgatagctt gtttggatat
     2821 ttcattagaa caatggtgcc aattattgac acagcggctc ccaataacag tgataacctg
     2881 attccaagtg gattagatat atctttaccg aaaaatacgt aaagtaccga tgctgctgct
     2941 gttgaaagcc caaggttaag ttttaagaaa agaccgttta ctccaaaata tatcccttcc
     3001 ctgttttctc cggttaactt ataatcgtaa tcgcataact ctgctataaa aacattggga
     3061 agaatcaaaa acacggcggt tggaatacca agaagccccc agaacacttt cccgtataag
     3121 tttttgtcaa atggtaaaat tcctgtaagc gcgagaccaa ttgaaaaaat ggaaaacagc
     3181 aataatcctg ttatcatgac attctttttg ccgtatttct ttgaaaaaaa cataacacct
     3241 acgaaagata atccggcgct tacaaaaagt ataataaaat ttaaccccga ttcttcaacg
     3301 ttgagtccca tcatggtttc gccaatatgc actgctgacg aacgaagtat gttgaacaca
     3361 aaccaccctg acatataact tagtaaaaaa attataaaat aactgttgga aagagttttt
     3421 tttatggaag ggattattgc taaagaagaa ggtactgatt tagtatattt tttttcgtta
     3481 accgcaagta tagccatcaa taaaaaaata aatccaataa tgcataaaac aataacagta
     3541 aattgataag attttgttaa tgtgtatttt tttctaaaaa ctccgattag tgcagaaccg
     3601 cctatcattg ctatagcccc accgataaga gcaaaatatc cctgcgcggt agtgattgat
     3661 aatcttttct cttcagtgtg ggcaagttca ggtattaaag caatatatgg tgcgacatac
     3721 attgtataaa acaggaaaaa tagtgagaac ataagggata aataaataat atttattacg
     3781 gaaggtcctg ctatcggtgg aaaaaatatt aatgccgttg atagggtaaa gggtataatc
     3841 gagattataa gaaaaagtcg g
//
LOCUS       DAOTGZ010000250         3850 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_3064
            NODE_28048_length_3850_cov_4.862978, whole genome shotgun sequence.
ACCESSION   DAOTGZ010000250 DAOTGZ010000000
VERSION     DAOTGZ010000250.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868088
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 3850)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3850)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:22:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,785
            CDSs (total)                      :: 2,751
            Genes (coding)                    :: 2,732
            CDSs (with protein)               :: 2,732
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 12 of 19
            Pseudo Genes (internal stop)      :: 5 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3850
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_28048_length_3850_cov_4.862978"
                     /isolate="AMR_MDS_3064"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..567)
                     /locus_tag="P5123_12365"
     CDS             complement(<1..567)
                     /locus_tag="P5123_12365"
                     /inference="COORDINATES: protein motif:HMM:NF012869.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter transmembrane domain-containing
                     protein"
                     /protein_id="HRX17101.1"
                     /translation="MKTFHSLIFLIGKQKTIFIFAILTLLLGVTFEASGRLVIRDIVD
                     TVLADKINLTNMQNLILLFTFCTLMQGTFHFISARWKTSATESIVRSIRNKLFNHIQQ
                     LSFHYHDHTPVGELVQRATSDVDSVRRFYGDQIPGILQIIFRCLIFFGAIALIDIRIA
                     LVTSIIIPVLTSISTFFFHKIYQAYDLLQ"
     gene            1097..2155
                     /locus_tag="P5123_12370"
     CDS             1097..2155
                     /locus_tag="P5123_12370"
                     /inference="COORDINATES: protein motif:HMM:NF024258.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase family protein"
                     /protein_id="HRX17102.1"
                     /translation="MYRYFSEMDRTYKNSLRIPLKNEKYIIFSDFHLGKRNSRDDFKE
                     NSSLIISVLKDYYFKNGYTLILNGDIEELQKNSINAVLNKWKDLYDVFALFAEDSRLF
                     KIVGNHDYKLYSRNYTDSVNSRLYDGLSLDYEGKTIFIFHGHQASKYSGFRNKLIGFI
                     LRYCAKPLGIKNIVRDYGNLKIHKTEERVYKYSRMKGVVSIIGHTHRPLFESLSDIDI
                     IIFTIERLLRNYTNAGKREKTGIEHEIEDNKKQLKRILSYDSMEGAIGSIYDREMIIP
                     VIFNSGCVIGPKGATGIEIDNGNISLIRWYNTYQKEKYYKQVRDERFTFGNRGAYRKI
                     LKQDSLDYIFNRIRLLTK"
     gene            complement(2156..2500)
                     /locus_tag="P5123_12375"
     CDS             complement(2156..2500)
                     /locus_tag="P5123_12375"
                     /inference="COORDINATES: protein motif:HMM:NF016955.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TerB family tellurite resistance protein"
                     /protein_id="HRX17103.1"
                     /translation="MIDTQAVLDIFFMAITVDNEIHISEIKTIKDIIENSPYKFPDDV
                     DKQIQNLTTKDQEIIKKNFDKAIAKLASEAIEDKRAILRLIMKIVDADNIMHQNEVRL
                     ISGFIKEWNIEV"
     gene            complement(2502..3716)
                     /locus_tag="P5123_12380"
     CDS             complement(2502..3716)
                     /locus_tag="P5123_12380"
                     /inference="COORDINATES: protein motif:HMM:NF013316.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="U32 family peptidase"
                     /protein_id="HRX17104.1"
                     /translation="MNNYEIVSPAGNLEKLKFAVLYGAHSVYFGGDSFNLRDKSKNFS
                     HQDIEEALRYCKAHKVKTTFLLNSFVHEDDINQLREYVKSIEHFDFDAIMISDPATLE
                     IVQKSQLNCRIYLSTQMSTLNHLSANFWHRNGISRIVLARETTLDDIKKIRDSSSVEL
                     EIFAHGAVCISYSGRCLLSRYLSGRDANQGACSHPCRWNFALVEEKRPGFHLEIIEHK
                     RDTHILSSKDLCLIHKINSFKDAGVNAFKIEGRMKSLYYAANVTRIYRHALDNPDLNN
                     SFKDFYNRELDLVSHRPYTDDLFNEFSDMENRDVPYIKKVVFLGYISNEEQTDNPVVK
                     TFNPIRPGDIIEAISPLNGDNILDTELTVLEIRNKDTQQPDELARPNSESYIKFDKSI
                     PGNSILRKKMAV"
     gene            complement(3703..>3850)
                     /locus_tag="P5123_12385"
     CDS             complement(3703..>3850)
                     /locus_tag="P5123_12385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005352652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="endolytic transglycosylase MltG"
                     /protein_id="HRX17105.1"
                     /translation="LNPVISDYLYFVARNDGTHYFSKTLSRHNKAVDYYQKNINNGFV
                     DEQL"
BASE COUNT         1267 a          604 c          715 g         1264 t
ORIGIN      
        1 ttgaagtaaa tcgtatgcct gatatatctt gtgaaaaaag aatgtcgaaa tagacgttaa
       61 cacaggtata attatcgatg ttaccagcgc tatccgtatg tcaattaaag cgattgcgcc
      121 aaagaatata agacatcgga atattatctg taaaatgccg ggaatctgat caccgtaaaa
      181 tcttcttact gaatcgacat ccgaagtcgc gcgctgaacc agctcaccaa ccggagtatg
      241 atcgtgataa tgaaaagata gctgctgaat atggttgaac agcttgttac gaattgatcg
      301 tacaattgat tctgttgcag atgtcttcca tcgagcactg ataaaatgaa aggtaccctg
      361 cataagagta caaaaagtaa aaagcagaat taggttttgc atatttgtaa gattaatttt
      421 atcggctagt acggtatcga caatgtccct tataactaga cgtccagatg cttcaaaagt
      481 cacacccaaa agaagggtta atatagcaaa aataaaaatg gtcttttgct ttccaattag
      541 aaatatgagt gagtgaaaag tcttcatgat ttgtctccat taattaaaaa aagccactta
      601 taaaagcggc ttttttacca tgcattaaaa tgcgggcata aaaaaagccg cggtcatccc
      661 gcggctggtt gtactgtttt ataataaaat ttagaaccaa tcgcaccgcg cggacgtacc
      721 tgtctccatg aaagagaaaa cattatgtgt aatagttcta atattcatga gtattcctta
      781 ttaaaattta gttctgtaac aattgattat gacacatagc aggtttaata ttaagttgtc
      841 aacaaaaaaa agtaatcgtg aaactttagt tgtaaaaaaa aattttgcta ttgaataagt
      901 atagaaatga ttcagtttgt aaaaagataa tatttataaa gaatatatcg ttttacttgc
      961 atttatgaaa aaatatttac catatttatg tagaataaat taataatatt tactatgata
     1021 tacttgtaat tatctagaaa tgcatacttg attatcttaa acaaatattt gatacttctg
     1081 taaggaaccg taaattatgt atagatattt ttcagaaatg gataggactt acaaaaattc
     1141 tttgcgaata cctttaaaaa atgagaagta tataatattt agtgatttcc atcttggtaa
     1201 aagaaattcg cgtgatgatt ttaaagaaaa ttcatctctg attatttctg ttttaaaaga
     1261 ctactacttt aaaaatggtt atacattaat tcttaatggt gatatagaag agcttcagaa
     1321 aaatagcata aacgctgtac ttaataaatg gaaagatctt tacgatgtat ttgccctttt
     1381 tgccgaagat agcaggcttt ttaaaattgt aggtaatcat gattataagc tttattctcg
     1441 taactatact gattcggtaa attcgagact gtacgacggt ctttcgcttg attatgaggg
     1501 taaaacaatt tttatttttc atggtcatca ggcttcaaaa tacagtgggt tccgaaataa
     1561 gcttataggt tttatattgc gttattgtgc caaaccactt ggaataaaaa atattgtcag
     1621 agattacggg aatttaaaaa tacataaaac ggaagagcgt gtttataagt actcgcgaat
     1681 gaaaggggtt gtttcgataa ttggtcatac tcatcgtccg ctttttgaat cgttatctga
     1741 tatagatatt attattttta caatagagag gttactgcgt aactatacaa atgcgggaaa
     1801 gcgtgaaaaa acaggaattg agcatgaaat agaagataat aagaagcaat taaaaagaat
     1861 tctttcttat gattcgatgg agggtgcaat tggcagtatt tatgatcgtg aaatgataat
     1921 accggtcatt tttaattcgg gctgcgttat tgggccaaaa ggtgctaccg ggattgaaat
     1981 tgataatgga aatatttctc ttattagatg gtataatacg tatcagaaag agaaatatta
     2041 taagcaggtg cgtgatgaga gatttacctt tggaaaccgg ggtgcataca gaaaaatact
     2101 taaacaggat tcactcgatt atatctttaa cagaataagg ctattgacaa aatagttaaa
     2161 cttcaatatt ccactctttg atgaatccgg aaataaggcg tacttcattt tggtgcatga
     2221 tgttatccgc atccacaatt ttcataatta aacgcaatat cgctcgcttg tcttcaatgg
     2281 cttccgatgc cagttttgca atggccttat caaaattttt tttaattatt tcttgatctt
     2341 ttgtcgttaa attttggatc tgtttatcaa catcatccgg aaatttatag ggggaatttt
     2401 caataatatc tttaatcgtt ttaatttcgg aaatatgaat ctcattatca acagttattg
     2461 ccataaaaaa gatatcaagt acagcctgtg tatcgatcat tttaaaccgc catttttttt
     2521 ctaaggatac tgtttccggg aattgatttg tcaaatttga tataggattc agagttaggt
     2581 ctagccaact cgtccggctg ctgcgtgtcc ttatttctaa tttcaagtac agtaagctcc
     2641 gtgtcaagta tattgtctcc gtttagggga gaaattgctt caattatatc gccagggcgt
     2701 atcggattaa aggttttaac gacaggatta tcagtctgct cttcattgct aatatagcct
     2761 aaaaagacca ctttctttat atacggtaca tcccgatttt ccatgtcact aaactcattg
     2821 aaaaggtcat ctgtataggg gcggtgactg acaagatcaa gctctctgtt ataaaaatct
     2881 ttaaaagaat tattaagatc cgggttgtca agtgcgtgtc tgtatattcg ggttacatta
     2941 gctgcataat agagagattt catacgcccc tctattttaa aagcgttaac acctgcatct
     3001 ttaaaactat taattttatg tataagacaa agatccttgg atgagagtat atgcgtgtct
     3061 cgtttatgtt caataatttc aagatgaaaa cctggtcgtt tttcttcgac tagtgcaaag
     3121 ttccaacggc aaggatgcga gcacgccccc tggtttgcgt ctcttcctga aagataccgg
     3181 cttaataaac aacgaccgga gtaagagata catacggcac cgtgtgcaaa aatttcaagt
     3241 tctaccgaac ttgagtcgcg tatctttttt atatcatcaa gagtcgtttc acgagcgaga
     3301 acaattcgag aaattccgtt tctgtgccag aagtttgcac taagatgatt aagtgtggac
     3361 atctgtgtag ataggtaaat acgacagtta agttgtgatt tttgtacaat ttcgagtgtt
     3421 gccggatctg atatcataat agcgtcgaag tcaaagtgct cgatggattt cacatattcc
     3481 cggagttggt tgatatcatc ctcatgaaca aacgagttta gtaaaaaggt ggttttgact
     3541 ttatgcgcct tgcaatatcg aagtgcttct tcaatatctt gatgagaaaa atttttagat
     3601 ttgtctcgca ggttaaacga atctccacca aaatagacac tatgagcacc gtaaagaact
     3661 gcaaatttta atttttcaag atttccagca ggtgatacta tttcataatt gttcatcgac
     3721 aaatccattg tttatgtttt tttggtaata atccacagcc ttgttatggc gtgacagagt
     3781 tttggaaaaa tagtgtgtgc cgtcatttct tgccacaaaa tagagataat cgcttattac
     3841 aggattaagt
//
LOCUS       DAOTGZ010000251         3814 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_3064
            NODE_28387_length_3814_cov_4.037776, whole genome shotgun sequence.
ACCESSION   DAOTGZ010000251 DAOTGZ010000000
VERSION     DAOTGZ010000251.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868088
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 3814)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3814)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:22:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,785
            CDSs (total)                      :: 2,751
            Genes (coding)                    :: 2,732
            CDSs (with protein)               :: 2,732
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 12 of 19
            Pseudo Genes (internal stop)      :: 5 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3814
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_28387_length_3814_cov_4.037776"
                     /isolate="AMR_MDS_3064"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(139..693)
                     /locus_tag="P5123_12390"
     CDS             complement(139..693)
                     /locus_tag="P5123_12390"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRX17106.1"
                     /translation="MKFLNSILTLLGAVVFYFVFYTLLKSVLPVLPDLNLFRQLDLTF
                     VVKGYRVPAFDMIFFSFGLWVFCHGVLRLASLSEKDHSSKDDLDAGEFGKIRHPVYGA
                     FMIIELGLFVSLRTVYSLCFMAVLIVFQIVASYIEDNYVLKKKFGDSYDEYCNSVKRR
                     FFTPVIIFYYLIAALLTFAGIYFM"
     gene            complement(690..2069)
                     /gene="argH"
                     /locus_tag="P5123_12395"
     CDS             complement(690..2069)
                     /gene="argH"
                     /locus_tag="P5123_12395"
                     /EC_number="4.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004319174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004056 - argininosuccinate lyase activity
                     [Evidence IEA];
                     GO_process: GO:0042450 - arginine biosynthetic process via
                     ornithine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="argininosuccinate lyase"
                     /protein_id="HRX17107.1"
                     /translation="MATKKMWGGRFEGKNSDVTERLSMSVHYDSRLYRQDIKGSKVHA
                     EMLKDMGILTHDEFESIVSGLDTVLKEIESGDFNFRSDYEDIHMNIEARLTELIGDAG
                     RKLHTARSRNDQVAVDTRLYIREETEKIESLLKKFILRLADIAEQQRETVMAGYTHMQ
                     IAQPVRLSQHLLAHAWALSRDLKRFETCRNMHNLCPLGVGALAGVNYVNNRKFLAERL
                     GFAGVTPNSMDTVADRDYIADILYASSLLGVHLSRICEELVLWSTFEFNYIRLSDQVT
                     TGSSIMPQKKNPDLAELIRGKSGRLTGNLVSLLTVMKGLPLTYNRDMQEDKEPLFDTV
                     DTVLLCLEGMIEMFKDIVFNKERMSETLRGNFSTATDIADYLVQKGVPFRTSHEIVGS
                     IVGYCEKEKRNFFELNLEELRKFSNAFEKDALINLDPAGSTERKQSEGGTSLSSVLNQ
                     IELIREIVR"
     gene            2395..2751
                     /locus_tag="P5123_12400"
     CDS             2395..2751
                     /locus_tag="P5123_12400"
                     /inference="COORDINATES: protein motif:HMM:NF018894.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0035438 - cyclic-di-GMP binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PilZ domain-containing protein"
                     /protein_id="HRX17108.1"
                     /translation="MADKRQHQRVYPDSEHPVEIQIMGKGFLDIIFAKDISIKGIGIE
                     VPHKFEGSDIDHPIELIITLPPNSCFKASGIIKHSNIEQTRKGIFGVQFTSIQKGYEQ
                     ILESYITKRISDGGLA"
     gene            complement(2775..3782)
                     /locus_tag="P5123_12405"
     CDS             complement(2775..3782)
                     /locus_tag="P5123_12405"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRX17109.1"
                     /translation="MEVYMNGQSVDIRFEEEKTVKEVAESMSKWAAERDLIFTGMLLD
                     DEFFVADNVPDRPIDKVGICDLGIQSKADLVISSLTEGISYCDRVVEYIGDCSGTDCF
                     DNSDLESFFGGIDWLIDVTTTVFSMLNLDLQEIKHMDRTVAEYLGDIGLLASELQKAA
                     DKKDGDTVLKLLDEQKQLFSLFKGIFKMAMLSDNIKDMVVRSIDSPDSLIKSLIESRD
                     NLPQQLENLEKISEAFQSGHDEEASSLMHPFLDYMYTYSRTSIQSAPVFGVDPDELIV
                     DGISISEKNRQINELLAEIVDVMESDDIISASDILEYELKPALESVPEYINQLLERIG
                     A"
BASE COUNT         1232 a          830 c          663 g         1089 t
ORIGIN      
        1 tgactgacaa ataactacta atcattaatc agcgaatttc aaacaaaacg gctaccggct
       61 attaaacaat aatcgccttt tgcacttcat caaaatacta accaggcacg attgctggga
      121 acatagcccc tttccgcgct acatgaaata gataccggca aaggtaagca aggcagcgat
      181 cagataatag aatataataa ccggtgtaaa aaaacgtctt ttcaccgagt tacaatattc
      241 atcatagctg tcaccgaatt ttttctttaa aacatagtta tcttctatgt agctagcaac
      301 aatctgaaat acaattaaaa ctgccataaa acaaaggctg taaacagtgc gcaatgatac
      361 aaacaatccc aattcgatta tcataaacgc cccgtaaaca ggatgacgta tcttaccaaa
      421 ttcaccagca tcaagatcat ctttagacga gtggtctttc tcggaaagag aggccagacg
      481 cagtactcca tgacaaaaga cccaaagtcc aaaagagaag aagatcatat caaaagcggg
      541 tacgcgataa cccttaacaa caaaggttaa atcgagctgc cgaaaaagat tcaaatcagg
      601 caaaacgggg agcacgcttt ttaaaagggt atagaagaca aaataaaata caacggctcc
      661 taaaagtgtt aatatagagt ttaaaaactt catctgacta tctcccttat caactctatc
      721 tgatttaaaa cacttgataa cgaagttccg ccttctgact gtttgcgttc agttgaacca
      781 gcaggatcca gatttattaa ggcatctttt tcaaacgcat tcgaaaattt acgaagttct
      841 tcaaggttca gttcgaagaa atttcgcttt tctttttcgc agtaacctac gattgaccca
      901 acgatttcat gtgaagtacg aaaagggaca cctttttgta ccagataatc agcaatatct
      961 gttgcagtag aaaaatttcc tcgcagagtt tcgctcattc tttccttatt aaaaactata
     1021 tctttgaaca tctcaatcat tccttcaaga cagagaagaa cggtatcaac ggtatcgaac
     1081 agaggctctt tatcttcctg catatcccgg ttatacgtaa gcggcaaacc tttcataacg
     1141 gtaagaagag aaacaaggtt tcctgttaaa cggccgcttt tcccccgaat aagctctgcc
     1201 aaatcgggat tctttttttg cggcataatt gatgatccgg ttgtcacctg gtcagagagt
     1261 cttatatagt taaattcaaa tgttgaccag agaacaagct cttcacatat acgagaaaga
     1321 tgaacaccca agagggagga agcataaaga atatctgcga tatagtccct gtctgcgact
     1381 gtatccatag agttgggcgt aactccagcg aagccaagac gttctgcaag aaacttgcga
     1441 ttattgacgt aattaacccc ggctaatgct ccaaccccta aaggacaaag attgtgcata
     1501 ttacgacaag tctcaaatct tttcagatct cttgacagcg cccaggcgtg agctaataga
     1561 tgctgtgaaa gtctaacagg ctgtgctatc tgcatgtggg tatagcccgc catcacggtt
     1621 tcacgctgtt gttcagcaat atctgcaaga cgcagaataa attttttcaa taaagactca
     1681 atcttttcag tctcttccct aatgtacagc cgggtatcta ctgcaacctg gtcatttcgc
     1741 gaacgggcag tatgaagttt tcgccccgcg tcacctataa gctcggttaa tcgagcttca
     1801 atattcatgt gtatatcttc ataatcagaa cgaaaattaa aatcgcccga ctcaatctct
     1861 tttaaaacag tatcaaggcc gctcacaatg gattcaaatt catcgtgtgt caaaatcccc
     1921 atatctttaa gcatttcagc atgaactttt gaacccttaa tatcctgacg atatagccta
     1981 gagtcgtagt gtactgacat tgaaagccgt tcggtaacat cggagttttt accttcgaac
     2041 cttccacccc acatcttttt tgtagccata tatcctccag tatcatgcga gagtactaat
     2101 attctaaaac gattatttta aaacggctct ttttgtaaat caataaaaaa ttaggaccaa
     2161 tcactaaatt ttaaaatatg aaaaacaatt ttaagcttta aagacagctt caaatttcag
     2221 cttggtaagc gatattcgtg gctcttttga aaaggaattt ttcgcaagtt ccttgttgaa
     2281 aattctccta ttcggaatta tctttatctt aaattattgc tatttcacgg tttaatgaat
     2341 atgtatggtt tttgttttat ttattgcaca atattaaaag gtaaaggcga tataatggct
     2401 gacaagagac aacaccaaag ggtatatccg gattctgagc atcctgtaga gattcaaatt
     2461 atgggtaaag gatttcttga cattattttt gcaaaggata taagcattaa gggtataggt
     2521 atcgaagttc cccataaatt cgaagggtct gatattgacc atccaataga acttataata
     2581 actcttccac caaacagttg tttcaaagcc agtggaatca ttaaacacag caacatcgag
     2641 caaacaagga agggaatttt cggagttcag tttacttcca tacagaaggg atacgagcaa
     2701 attcttgagt catatattac caaaagaata agcgatggcg ggcttgcata acaagtaccg
     2761 ccatgtctct cttcttaggc acctattctt tcaagtagtt gattgatata ctctggcaca
     2821 gattcaagtg ccggcttgag ctcatattca agaatatccg aagccgaaat aatatcatca
     2881 ctttccataa catcgacgat ctcggccaag agctcattta tctgcctgtt tttttcggaa
     2941 attgaaatac cgtctacgat aagctcgtct ggatctaccc caaaaacggg cgcactctgt
     3001 attgatgtac gcgaataggt gtacatataa tcaagaaacg gatgcataag agagctcgct
     3061 tcctcatcat gtccactctg gaaagcctcc gatatttttt caagattttc gagctgctga
     3121 ggcaaattgt ctcgggactc tatcaatgat ttgatcaaag agtcaggcga atcgatacta
     3181 cgtacgacca tatcttttat attatcagac aacattgcca ttttaaaaat tcccttaaaa
     3241 agagaaaaaa gttgcttttg ttcatcaaga agttttagca cagtatcgcc atcttttttg
     3301 tcggccgctt tctgcaattc tgacgctaaa agtccaatat cacccaaata ttcagcaaca
     3361 gttctatcca tatgcttgat ctcctgaaga tcaagattca acatcgaaaa aacagtagta
     3421 gttacatcaa ttaaccagtc aattccaccg aaaaaagatt caaggtcgga attatcgaaa
     3481 cagtcggttc cactgcaatc accgatatat tccacgacac gatcacaata cgatatcccc
     3541 tcggtcaatg atgatattac cagatcggcc tttgattgaa taccgagatc gcagatccca
     3601 accttatcta tgggtctatc cggcacatta tcagccacaa aaaattcatc atcaagcagc
     3661 attccagtaa aaataaggtc tcgttcggcc gcccattttg acatagactc ggctacttcc
     3721 tttacggtct tttcctcttc gaaacggata tctacagact gcccgttcat atatacttcc
     3781 atatattctc ccgataacta tccaatttta acca
//
LOCUS       DAOTGZ010000252         3803 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_3064
            NODE_28484_length_3803_cov_4.051494, whole genome shotgun sequence.
ACCESSION   DAOTGZ010000252 DAOTGZ010000000
VERSION     DAOTGZ010000252.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868088
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 3803)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3803)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:22:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,785
            CDSs (total)                      :: 2,751
            Genes (coding)                    :: 2,732
            CDSs (with protein)               :: 2,732
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 12 of 19
            Pseudo Genes (internal stop)      :: 5 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3803
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_28484_length_3803_cov_4.051494"
                     /isolate="AMR_MDS_3064"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..369
                     /locus_tag="P5123_12410"
     CDS             <1..369
                     /locus_tag="P5123_12410"
                     /inference="COORDINATES: protein motif:HMM:NF012568.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyprenyl synthetase family protein"
                     /protein_id="HRX17110.1"
                     /translation="IRDDYIGVFLSENESGKSEACDLHEQKVTTVVSEAFMAMAPGQQ
                     QIFATLFRKDKKSSEVVFNLRKMITESGASERTITRLLKISEKFENDVRKLALDPASK
                     DLFTDIVKRICLLPAEASEE"
     gene            369..1271
                     /locus_tag="P5123_12415"
     CDS             369..1271
                     /locus_tag="P5123_12415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010937965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine acetyltransferase"
                     /protein_id="HRX17111.1"
                     /translation="MEKNKENRFFIKELASTLATHEVLSTMYHQDVHSVPMPSLEKLK
                     IIVNKLRSVLFPGYFGTASMRHGSVEFYIGALLDELYELLSVQIRRGFCFICNERSEK
                     CKVCEDKSAELCEEFLKKLPQIRHLLSTDAKAGYEGDPASFSVGEIIFSYPSIKALTN
                     YRIAHELFKLDVPLIPRIISELAHSETGIDIHPGAKIGESCFIDHGTGVVIGETCRIG
                     NNVRIYQGVTLGAKSFPLDENGNPIKGIDRHPKVGNNVVIYAGATILGTVMIGDGAEI
                     GGNVWITRDVPPGGKVRAQTYGNL"
     gene            complement(1446..1595)
                     /locus_tag="P5123_12420"
     CDS             complement(1446..1595)
                     /locus_tag="P5123_12420"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRX17112.1"
                     /translation="MVHSRHDMQNWKSEQISYFKRYLDSFDKGSEQYNVLRKGISELE
                     KTDIL"
     gene            1800..>3803
                     /locus_tag="P5123_12425"
     CDS             1800..>3803
                     /locus_tag="P5123_12425"
                     /EC_number="3.1.11.5"
                     /inference="COORDINATES: protein motif:HMM:NF016169.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease V subunit gamma"
                     /protein_id="HRX17113.1"
                     /translation="MSFYVYQSNRLEKVIPTIASVLAEKGSTQKGSKAQELLSVDYCL
                     VQSIGMGRYFALELASELGIFANYKMVYPNAFMRLLASHLKIDDAADSKESIGWKIFS
                     IIKNGEIENEILAGYIESARIDNKKKYDELKLFTFAYSLADLFDQYSLYRPSMVTLWD
                     RGEPVKTINNIQQPLDKDELWQYELWRKVYDGTMIHKAEIREKIISRINGREDLPFDR
                     FFILGVSTIPLFFLEVIYTISKYCDVYAALLSPSDSYWEDIVNKKYRFFKILEPDLKA
                     KKESTGKSDNHTLTGEDNNFDDYYFTGNTILGSNGMLGRDFNRILSDNFEGSIVQNDS
                     YYFTEESDNILGNIQRDIRELNDRGIFGDKTKKRFIYDNDDRSLQIHGVHSKRRELEV
                     LHDNLLEIMQKNPDIELRDILVMTPSVYDYAPYIKAVFEEFRGTKLYLDYSIADLALD
                     SDNETVPAFLKIISLYKGRFTSGDIISLLRLKPLSAHAKIDDENIENVAEMLNAARFR
                     WGRDGDDRKELLDIEYEEFSFAHAFKRIIHSTIFVRDFDQTYNGVFPSDDFDRDSSVL
                     AGCVLDFVLKLEDFTRECSKPRPVNQWEELLRKMVNDLFSNSNDFSQSRDTLFESISA
                     VCANSAKASDIVSFEIIESLLITHLSGESRAFGFIDGRITFCEM"
BASE COUNT         1188 a          639 c          814 g         1162 t
ORIGIN      
        1 attcgtgatg actatattgg tgttttttta tcagagaatg aatcgggtaa gtccgaagcc
       61 tgtgatctgc atgagcagaa agttacaaca gttgtttcag aagcctttat ggcaatggca
      121 cccggtcagc aacagatatt tgccacgctc tttagaaaag ataaaaagag cagtgaagta
      181 gtttttaatc taagaaaaat gataactgaa agcggcgcgt cggaacgcac aataacccgt
      241 ttgctgaaaa tatcagagaa gtttgaaaat gacgtgcgta agcttgcttt agatcctgca
      301 tcaaaggatc tgtttacaga catagttaaa aggatctgcc ttcttccggc ggaagcatca
      361 gaggaataat ggaaaaaaat aaagaaaacc gtttttttat aaaagagttg gcctcgacat
      421 tggccacgca tgaggtgctt agcacaatgt atcatcaaga tgtgcattcc gttccgatgc
      481 cgtctcttga aaaacttaag attattgtta acaaattacg ttcagtactc tttcccgggt
      541 actttggaac agcttccatg cgacacggtt cggtagaatt ttatattggt gcgcttttgg
      601 atgaacttta tgagttatta tcggtacaga tacgcagggg attctgtttc atctgcaacg
      661 agcgtagtga aaaatgcaag gtttgcgagg ataaatcggc cgagttatgt gaagagtttt
      721 tgaagaagct accgcaaatt cgtcatcttt tatcaacaga tgccaaggcc ggttatgagg
      781 gagatccggc atcttttagt gtgggcgaga taatattcag ttatccaagc attaaggctt
      841 taaccaatta ccgcatagca catgaactgt ttaaacttga tgttccactt atacctcgta
      901 taattagcga gcttgcacat tctgaaacgg gtatagatat tcacccagga gcaaaaatcg
      961 gagaaagctg ctttatcgat catggtacgg gagttgtaat tggagaaacc tgccgtatag
     1021 gtaataacgt tagaatatac cagggagtga ctcttggtgc gaagagcttc cctttggatg
     1081 aaaatggtaa tccgattaaa ggaatagatc gccaccctaa agtcggaaac aatgtggtga
     1141 tatacgcagg agcaacaata ctcggaacgg taatgatcgg tgacggtgcc gaaataggcg
     1201 gcaatgtctg gattacgcgt gacgtgcctc caggaggaaa ggtcagagca cagacatatg
     1261 gcaatctcta atacttaact tatcaacaag gaacttgcga aaaatccctt tatatgtggg
     1321 ctttagacgg catagccgtt ctaaaaatgg taatttttcg aagttccctt acgataaata
     1381 aaaaaaggtt cccgatattt acggaaacct tgtgaaatca ttataggtag tcactaaaaa
     1441 taattttaga gaatatctgt tttttcaagt tcactgatgc cttttcttag cacgttatac
     1501 tgttcagaac ctttgtcaaa actatccaga tatcttttaa aatatgatat ctgctcgctt
     1561 ttccagtttt gcatgtcatg acgtgaatga accatttgtg cctcctatgt ttaatttaga
     1621 cttttgcgat gtcaggatgc ggaataaccg gtattattaa atatgataag cagtcttact
     1681 attttattta catttaggta ttgattaaac cgccgcttac aatattaaat atcggactga
     1741 aagtgttatg agcatataat ttttttgatt aagcgatata atcatctgga gtctatatta
     1801 tgtcatttta tgtttatcag agcaatcgac ttgaaaaggt tatacctaca attgcttctg
     1861 ttcttgctga aaaaggtagt acacaaaagg gctccaaggc tcaggagctg ctttcagttg
     1921 attactgtct ggtgcaaagt atcggtatgg gacgttactt cgcgcttgag ttggcaagcg
     1981 aacttgggat atttgcaaac tataagatgg tttatcctaa tgcttttatg cgcctgcttg
     2041 cgtcgcatct taaaatagac gacgcggcag attcaaaaga gagtataggg tggaaaatat
     2101 tctcaattat aaaaaacggt gaaattgaaa atgagatatt agccggatat attgaatcgg
     2161 ccagaattga taataaaaaa aagtacgatg aattaaaact ttttacattt gcatactcct
     2221 tggcagatct attcgatcaa tattcattgt atcgcccttc aatggttact ctctgggaca
     2281 ggggagagcc ggttaaaacg attaataata ttcaacagcc tttggataaa gatgaacttt
     2341 ggcagtatga actctggcga aaggtctacg atggcacaat gatacacaag gccgaaataa
     2401 gggaaaaaat tataagccgg atcaatggcc gagaagacct tccttttgac agattcttca
     2461 ttcttggagt atcaacaata ccgctctttt ttctcgaagt aatatatact atttcaaaat
     2521 attgtgatgt atacgcggca cttctttcac cctcagactc gtattgggaa gatattgtta
     2581 acaagaaata ccgctttttc aagatattgg aaccagattt aaaagccaag aaagaatcta
     2641 ctggtaagag tgataatcat acgcttactg gcgaagataa taattttgac gactattatt
     2701 ttaccggaaa cacaatactt gggtcgaacg gaatgcttgg gcgcgatttt aaccgtattc
     2761 tttcagataa ttttgaagga agtatagttc aaaatgacag ttactatttt actgaagaga
     2821 gtgataatat tcttggcaac attcaaagag atattcgtga attaaatgat cgtggtatct
     2881 ttggagataa aacaaaaaaa aggtttattt acgataatga tgaccgctca ttacaaattc
     2941 atggtgtgca ttccaaacga agagagttag aggtgcttca tgataatctt ctagagataa
     3001 tgcaaaagaa tccggatatc gagctaaggg atattcttgt aatgacacct tcggtttatg
     3061 attatgctcc gtatataaaa gcagtatttg aagaattcag gggaacaaag ctttatctgg
     3121 actattcgat tgccgatctg gctcttgaca gcgataacga gactgtcccc gcatttttga
     3181 agattatttc actttataaa ggaaggttta cttccggtga tattatttct cttttaaggt
     3241 taaagccttt atcagcgcat gcgaaaatag atgatgaaaa catagaaaat gttgctgaaa
     3301 tgctgaatgc agcacgtttt agatggggaa gggacggaga cgatcgcaag gagcttctgg
     3361 atattgaata tgaagagttc tctttcgcgc acgcatttaa acgaattatt cacagtacaa
     3421 tatttgtccg tgattttgat caaacatata atggagtttt tccttcagat gattttgaca
     3481 gggacagttc tgttttagcc gggtgtgtac tcgattttgt attaaaacta gaagatttta
     3541 caagggagtg ttccaaacca agacctgtta atcaatggga agagttgctt cgtaaaatgg
     3601 ttaacgatct ttttagtaat agcaacgatt tttcacaatc tagggatacg ctttttgaat
     3661 ctatatcggc tgtttgtgcc aatagtgcca aagcttcgga cattgtatct tttgaaatta
     3721 ttgagtcgct tcttattacc catctttcag gtgaaagtag agcctttggg tttattgatg
     3781 ggcgcataac gttttgtgaa atg
//
LOCUS       DAOTGZ010000253         3797 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_3064
            NODE_28540_length_3797_cov_4.086050, whole genome shotgun sequence.
ACCESSION   DAOTGZ010000253 DAOTGZ010000000
VERSION     DAOTGZ010000253.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868088
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 3797)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3797)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:22:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,785
            CDSs (total)                      :: 2,751
            Genes (coding)                    :: 2,732
            CDSs (with protein)               :: 2,732
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 12 of 19
            Pseudo Genes (internal stop)      :: 5 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3797
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_28540_length_3797_cov_4.086050"
                     /isolate="AMR_MDS_3064"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1372
                     /locus_tag="P5123_12430"
     CDS             <1..1372
                     /locus_tag="P5123_12430"
                     /inference="COORDINATES: protein motif:HMM:NF013618.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="transglycosylase SLT domain-containing protein"
                     /protein_id="HRX17114.1"
                     /translation="VQYGKMLSAEADYLYGRSPASAISLYVKLIELDQESELQFSRVC
                     RYLYKRGRSNTKSYLKKFRKKYPDSDNAKLFEWYEARLSIVDNNFKEALPVLRNIAED
                     TSHKYYGNAMYWLYYYHREKDSDIAFDYFRKGVVYSPDSWYMWMLADKLKKDFSVDKL
                     EKSFSEAISSTDYESALFTHFMLYLLQRDNAKKERRLELIKTAGIYPYNVLDDFMLSV
                     KNECLAKQMELYFDAGYEDGITGLLHYMDESEDNRKLKYLLLSRFGDETSHYHYAFLG
                     TIRLLREFGIGEEITLLEDRLVKRLFPKAYSEIVKKTGKKYPKLDHNMIYSVMKSESA
                     FRHDAVSFVGATGLMQVMPATGGDIAKSLKMNSYDLKDPEVSILFGFYYMDWLRRIYD
                     GEFDQMLGGYNAGPGNMNKWKKSFDVSDKIFFAEKVPFDETRFYMFKNQKYLIQYNTI
                     YGENGD"
     gene            1440..2264
                     /locus_tag="P5123_12435"
     CDS             1440..2264
                     /locus_tag="P5123_12435"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HEAT repeat domain-containing protein"
                     /protein_id="HRX17115.1"
                     /translation="MNLLITCTKGIRISGLSLLVIASVSIVSCSRGMSVKQINSAVLS
                     AQKIYDSSSKWQDRERAIKSVKPFETKRALELKMAATEDTHSKVRVQAVMGLAPFVRD
                     DNVKDKLMLMSQKDREIPVRYASLKTLANNPDISMYGLFEKYTYDTDWLFREIAYRGI
                     MGITDEQMNEKSTALLIRGLSDSQETVQITILKNIKLQNDELYQSIRKNFFIKDYEKK
                     ITLLCATLKAVKGYNMDIRVRERVEQLILHQNKEVRVLALRALKSEPDYVNRNNKK"
     gene            complement(2268..2993)
                     /locus_tag="P5123_12440"
     CDS             complement(2268..2993)
                     /locus_tag="P5123_12440"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRX17116.1"
                     /translation="MKKGFVISLFTALTLSFLAFAQAVDGPDHLAKDVIKRLSYENFK
                     NIKLLTAAIFNYGGGEGEFDKLVQSYSEASSLYFSREYERSAKKFQENELEIRETAMN
                     IAKIYKEDSEAMQKEIIERDVRTRISKSLAGKKANESKKKMMSQSTASISLGKDNYER
                     TRPIEAIEAYRIAKDRMLTYMYIEADDLPQDIINECRKDRFERDKCLRDAKEKRRAEV
                     RERYEKIISDNDNKVYISKEKAK"
     gene            3145..3726
                     /locus_tag="P5123_12445"
     CDS             3145..3726
                     /locus_tag="P5123_12445"
                     /inference="COORDINATES: protein motif:HMM:NF013073.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="C40 family peptidase"
                     /protein_id="HRX17117.1"
                     /translation="MRKMFSVIIFILCLVTFIVAFAQPVSREEKTVRMSLIMVANKCV
                     GIGYQFNGTTPKGFDNAGFVLYVYQKNGITLPRTVKGMFSAGDYVDTKDAKPGDLLFF
                     MEDPVTGSSITHVGIYLGKDKFVHMPGEGAYVRIERLVKDSKWSARFAGVRSYHKLFD
                     EFIEKESNLSDKKEKVQALKNMDPEVQFETSDM"
BASE COUNT         1184 a          617 c          814 g         1182 t
ORIGIN      
        1 ggtgcaatac ggaaagatgc tctccgcaga ggctgactac ttgtacggca gaagcccggc
       61 ttcggcgata tcactatatg tgaaattgat tgagcttgat caggagagtg agctgcaatt
      121 ttcgcgtgtt tgccgctatc tttataaaag gggaagatct aatacaaaga gttatctgaa
      181 aaaatttcga aaaaaatatc cagacagcga caatgccaaa ctatttgagt ggtatgaggc
      241 aaggttaagt attgtagata ataattttaa agaggctctt cctgttttaa gaaatattgc
      301 ggaagacact tcacataaat attacggaaa cgcgatgtat tggctatatt actatcaccg
      361 tgagaaagat agcgacatag cttttgatta tttcagaaaa ggagtggttt acagccctga
      421 ttcttggtat atgtggatgc tcgcggataa actcaagaag gatttctcag ttgataagtt
      481 ggaaaaaagt ttttctgaag caatatcatc aacagattat gaatcggcct tgtttactca
      541 ttttatgctt tacctgcttc aaagagacaa tgccaaaaaa gagaggcgcc ttgagcttat
      601 taagacggct ggtatttatc catataacgt attggatgat tttatgttgt ctgttaaaaa
      661 tgaatgttta gcaaaacaga tggaactata ttttgacgca ggatatgagg acggtattac
      721 gggtcttctg cactatatgg atgaatccga agacaacagg aaacttaaat atctgcttct
      781 ttcgcgattt ggagatgaaa catcgcatta tcactacgcg tttttgggca caattcgtct
      841 gttgcgtgag ttcggtattg gagaagagat aactttattg gaagacaggc ttgttaagag
      901 actttttcca aaagcgtata gtgaaattgt aaaaaaaaca ggaaaaaaat atcccaagct
      961 tgatcataat atgatctatt cggtaatgaa atcggagtct gcttttagac atgacgcggt
     1021 ctcttttgtg ggggcaacag ggcttatgca ggtgatgcca gccaccggag gagatatagc
     1081 aaaatcttta aaaatgaata gctacgattt aaaagatcct gaggtttcaa tattattcgg
     1141 attttattat atggattggt taagacgtat ctatgatggt gagtttgatc agatgttggg
     1201 cggttacaat gctggtccgg gtaacatgaa taagtggaaa aaaagtttcg atgtttctga
     1261 caaaatattt tttgccgaga aggttccctt cgatgaaaca agattttaca tgtttaaaaa
     1321 tcagaaatat ctgatacagt acaataccat atatggtgaa aacggtgatt aaagttgcca
     1381 aaaattttgt gatatactgt aatactggtt cactcaaagt attagtatta agcaggtttt
     1441 tgaatttgct gataacctgt accaaaggca taagaatttc aggattgtct cttttggtta
     1501 tcgcaagtgt atcaattgtt tcgtgcagcc gaggaatgtc ggttaaacag attaactcag
     1561 ctgtattgag cgcacaaaag atatatgatt catcctcaaa atggcaggat cgtgaaagag
     1621 ccattaaatc tgttaaacca tttgaaacaa aaagagcact tgagctcaag atggcagcaa
     1681 cagaggatac ccattcaaag gttcgcgttc aggctgtaat gggattggct cccttcgtca
     1741 gagatgataa tgtgaaagat aaattgatgt tgatgtctca gaaggatagg gaaatacccg
     1801 tgaggtatgc ttctcttaag actctcgcaa ataatccgga tatttcaatg tacgggttat
     1861 ttgaaaaata cacttatgat acggattggc ttttcaggga gatagcatac cgaggaatta
     1921 tgggtataac agacgaacag atgaatgaaa aatcaacagc ccttttaata aggggattaa
     1981 gtgatagtca ggagactgtt caaataacga ttcttaaaaa catcaaattg caaaatgatg
     2041 aattatatca gagcattcga aaaaattttt ttattaaaga ttatgaaaaa aaaattacgt
     2101 tattgtgtgc aacattaaaa gcagtaaaag gctataatat ggatatcagg gttcgtgaaa
     2161 gggtagagca gctgattttg catcaaaaca aggaagttcg agttttggct ctacgggccc
     2221 taaaatcaga gcccgattat gttaaccgta ataataaaaa ataatcttta cttggctttc
     2281 tctttggaga tataaacctt gttgtcatta tctgagatta ttttttcata tctttcacgt
     2341 acctcggcac gtcttttctc tttggcatca cgtagacact tgtctcgctc gaaacgatcc
     2401 ttgcgacatt cgttaataat atcctgagga agatcatcgg cttcaatgta catgtacgtc
     2461 agcatacggt ctttagctat gcggtatgct tcgatagctt cgattgggcg tgtgcgttcg
     2521 taattatcct tgccaagtga tatcgatgca gttgattgcg acatcatctt tttcttggat
     2581 tcgtttgctt tctttccggc aagagattta ctgattcttg ttctgacatc acgctcaatt
     2641 atctcttttt gcattgcttc ggaatcttcc ttgtatatct ttgcgatatt catggctgtt
     2701 tcgcgtattt caagttcatt ttcctggaat tttttggctg agcgctcgta ttcgcgtgaa
     2761 aaatagagag aagaagcttc agagtagctt tgaacaagtt tatcgaactc gccttcacct
     2821 ccaccgtaat taaatatcgc cgctgtcagc agtttgatat ttttgaaatt ctcatagctt
     2881 aatcttttaa taacatcctt tgcaagatgg tccggcccgt ctacagcctg ggcaaaggct
     2941 aaaaatgaga gagtaagtgc cgtaaaaagc gatataacga atcctttctt cataatatgg
     3001 gctccttaaa gaaatactat tattatttat tataacatcc gataaattta gtgtgtactc
     3061 aaataaatat tgttaaattt accaataaat gacctaaaaa gtcaaggaaa aataacagta
     3121 tcatctgtca cctggaggat tcgcatgagg aaaatgtttt ccgtcattat atttatatta
     3181 tgccttgtta cgtttattgt cgcattcgcg cagcctgttt cccgagagga aaagaccgta
     3241 cgaatgtctt taataatggt cgcgaataaa tgtgtgggaa ttggttatca atttaatggt
     3301 acaactccga agggatttga taatgctgga tttgtgctat atgtttatca gaaaaacggc
     3361 ataaccttgc ctcgaacagt aaagggaatg tttagcgctg gtgattatgt tgatacaaaa
     3421 gacgctaaac ccggagattt gctctttttt atggaagacc cggttacagg ctcgtcgatt
     3481 acccatgtgg gtatttacct gggtaaagac aagtttgtgc atatgccggg agaaggtgct
     3541 tatgtgcgta ttgaaagact tgtcaaagat tcaaaatgga gtgcaagatt cgccggggta
     3601 agatcgtatc acaaattatt tgatgaattt atcgaaaagg agtcaaattt atctgataaa
     3661 aaagaaaagg ttcaggctct caaaaatatg gatcctgaag tacagttcga gacttcagat
     3721 atgtaattgc tatattatgc gtgttatcag gattgaaagg tcgtcttccg gttcgttgct
     3781 tccggtaaac tggtata
//
LOCUS       DAOTGZ010000254         3748 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_3064
            NODE_29037_length_3748_cov_4.568914, whole genome shotgun sequence.
ACCESSION   DAOTGZ010000254 DAOTGZ010000000
VERSION     DAOTGZ010000254.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868088
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 3748)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3748)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:22:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,785
            CDSs (total)                      :: 2,751
            Genes (coding)                    :: 2,732
            CDSs (with protein)               :: 2,732
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 12 of 19
            Pseudo Genes (internal stop)      :: 5 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3748
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_29037_length_3748_cov_4.568914"
                     /isolate="AMR_MDS_3064"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            363..827
                     /gene="rimI"
                     /locus_tag="P5123_12450"
     CDS             363..827
                     /gene="rimI"
                     /locus_tag="P5123_12450"
                     /EC_number="2.3.1.266"
                     /inference="COORDINATES: protein motif:HMM:TIGR01575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008999 -
                     peptide-alanine-alpha-N-acetyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosomal protein S18-alanine
                     N-acetyltransferase"
                     /protein_id="HRX17118.1"
                     /translation="MKIIKVNREQIHSVYQLEIENYSSPYNQNSLLNELFIEDSLFYC
                     NIDLDNNVTGYIIARLVLGECEIHRITVSKDYRRQGIAQNLLNCIYKICIDQTVHAVF
                     LEVNENNRPAINFYKKNNFIQVGQRKKYYGDSDALILQKRISYVNDPIERIC"
     gene            796..1383
                     /gene="yihA"
                     /locus_tag="P5123_12455"
     CDS             796..1383
                     /gene="yihA"
                     /locus_tag="P5123_12455"
                     /inference="COORDINATES: protein motif:HMM:NF014474.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTP-binding protein
                     YihA/YsxC"
                     /protein_id="HRX17119.1"
                     /translation="MLTIQSSEFVKSVVGSNYPDKNLPEYAFLGKSNSGKSSLINMLL
                     NRKNLVKTGRTPGMTKMLNYFLINKKFHIVDMPGYGFAKVPISVKKEFQKLLRQYIEE
                     RRKNIKILFLLADIRRIPGEDEFFFLDLLAKNGVPAALTLTKVDKLSKSQQIRQIDLI
                     LKATGLDKKYIFTTSALKKTGREDILYAIENSLSN"
     gene            complement(1421..2536)
                     /gene="dnaJ"
                     /locus_tag="P5123_12460"
     CDS             complement(1421..2536)
                     /gene="dnaJ"
                     /locus_tag="P5123_12460"
                     /EC_number="1.8.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004786635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA];
                     GO_process: GO:0009408 - response to heat [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaJ"
                     /protein_id="HRX17120.1"
                     /translation="MASKRDYYEILGVSKSAGEDEIKKAYRKLALQYHPDRNKGNKEA
                     EEKFKEATEAYEVLRDSKKRSQYDKFGHEGVGGFEGFGRGAYSDFSDIFGDFDLGDIF
                     EGFFGGGAFGGRSGSTRRAQRGADIQYDLSLTLEESAVGHEVQIEIPRHEHCETCSGS
                     GSAPGSKSATCPVCNGTGQVRQTQGFFSVTQTCYKCKGEGKIIANPCKTCNGTGLKVQ
                     KRKITVKVPPGVESGSRLKISGEGEQSSGGGVRGDLYVLLHIRKHELFERHGSDLLID
                     VSISIPLACLGGEILVDTIYGKKIKLKVPAGTSSGQIFRLKGQGLPYLGSYGKGDQHV
                     RVNIDIPKKLSARQKELLKEFAKISGEAFDQSRPFYK"
     gene            complement(2929..>3748)
                     /locus_tag="P5123_12465"
     CDS             complement(2929..>3748)
                     /locus_tag="P5123_12465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004513688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="Hsp70 family protein"
                     /protein_id="HRX17121.1"
                     /translation="LTGDVKGLLLLDVTPLSLGIETLGGVMTKLIERNTTIPTKKSQV
                     FSTAADNQPAVTVHVLQGERELASQNRTLGRFELTDIPPAPRGVPQIEVSFDIDANGI
                     VHVSAKDLGTGKEQKIRIESSSGLSEDEINKMVKDAESHASEDKKAKELIEAKNESDT
                     LIYSLEKLLKDNADKVEESEKMKIESEIEKLKSAMAGSDVEAIKAAKSAVEQSSHQFA
                     QRIYQQAGAGAGQPGADAAQSEPETQGGGESQNNSQSQGNVYDADYEVVDDDKK"
BASE COUNT         1205 a          776 c          591 g         1176 t
ORIGIN      
        1 tttatgtaaa caacacattt tttttataat ttggttaata ttgctaattg ctatgctgta
       61 acaacttact taatccttat ctgttataac atttatttag taaagaagat attttttaca
      121 tttattgatc atgaaatttt ataaaatagt gaaagtaaaa caatattttg atctataaat
      181 ctataaaaac atacagtaat ctctgacatt taacttttta acaagttccc atatgataat
      241 ttgtctatcg acaaggaact tggggaaatt attcaataat tctttaaatt caattgccaa
      301 tattatcata ttatgtttct aaattatcct aaaataggaa aaaatccgat aaggtgatca
      361 tattgaaaat aataaaagta aatagagaac agatacatag cgtttatcaa cttgaaattg
      421 aaaattacag ttccccatat aaccaaaact ctttactaaa tgagttattc attgaagatt
      481 ccctctttta ttgcaatatc gacctggaca acaatgtaac cggatatata atcgcacgcc
      541 tagttcttgg agaatgcgag atccaccgta ttaccgtaag caaagattat cgtcgtcaag
      601 gcatcgctca aaatcttcta aattgtattt ataaaatatg tattgaccag accgtgcacg
      661 cggttttttt ggaagttaac gagaataatc gtcccgcaat caatttttat aaaaaaaaca
      721 actttattca agttggacaa agaaaaaaat actacgggga ctctgacgcg ttgatacttc
      781 aaaaaaggat atcctatgtt aacgatccaa tcgagcgaat ttgttaaatc tgttgtcggt
      841 agtaattatc cggataaaaa tttaccggag tacgcatttt tggggaaatc aaacagcgga
      901 aaatcatcgc tgataaacat gctacttaac agaaaaaatc tggtcaagac tggtcgtact
      961 ccaggcatga caaaaatgtt aaactacttt ttgataaaca aaaaattcca tatagttgat
     1021 atgcccggtt acggttttgc gaaagttcct ataagtgtaa aaaaagagtt tcaaaaactg
     1081 ttaaggcagt acattgaaga acgcagaaaa aacattaaga ttcttttttt gctggcggat
     1141 attagaagaa taccgggtga agatgaattc ttctttttgg atcttcttgc aaaaaacgga
     1201 gttcccgcgg ctcttaccct taccaaggtt gataagcttt caaagtctca gcagataaga
     1261 caaatagatc taatattgaa agcaaccggt cttgataaaa aatatatatt tacaacttcg
     1321 gctttaaaaa aaaccgggcg tgaagatatc ctctatgcta tagaaaactc tttatcaaat
     1381 tgattactat tttattatgt aaaaaagcca ctgccgagac ctatttgtaa aatggtctcg
     1441 actgatcaaa tgcttctcca cttatcttgg caaattcttt aagcagctct ttttgacgag
     1501 ccgacaactt tttaggtata tcgatgttta ctctcacatg ttgatcaccc ttgccgtaag
     1561 aaccaagata cggtaacccc tgtcccttca aacggaatat ctgtccgcta gacgtaccgg
     1621 caggaacctt aagttttatt tttttaccat atattgtatc caccagaatt tctccgccaa
     1681 gacaggccaa aggtatagat atggaaacat caatcaaaag atcacttcca tgtctttcaa
     1741 aaagctcatg ctttcgaata tgaagcaaaa cataaagatc tccgcggacg cccccaccac
     1801 tgctttgctc tccttctccg cttatcttaa ggcgagaacc agactctact ccaggtggaa
     1861 ctttaacagt gatcttgcgt ttttgtactt ttaagcccgt tccattacaa gttttgcaag
     1921 gatttgcaat aatcttgccc tctcctttac acttataaca agtctgtgtt actgagaaga
     1981 agccctgtgt ctgacggacc tgtccagttc cattacagac cggacatgtt gctgattttg
     2041 aaccaggagc agaacctgag ccagaacagg tttcacaatg ttcatgtcgt ggaatttcga
     2101 tttggacctc atgtccaaca gctgattcct caagagttaa actaagatcg tattgaatat
     2161 cggctccacg ctgtgccctg cgagtcgaac ctgaacgacc tccgaaagca cctccgccaa
     2221 aaaatccttc aaagatatct cctagatcaa aatctccgaa aatatcagag aaatcagagt
     2281 aagcaccgcg accaaatcct tcaaatccac ctactccttc atgaccaaat ttatcatact
     2341 gggatctttt ttttgaatca cgtaaaacct cataggcttc agtcgcctct ttaaacttct
     2401 cttcagcctc tttattgcct ttattgcgat ccgggtgata ctgcaatgca agtttacgat
     2461 aagctttttt aatctcatct tcaccggcac tcttggaaac acccagaatt tcataatagt
     2521 ctcttttaga agccaaaatt acctcactta taacaacacg gtattaaatt aaatcaaatc
     2581 ttagaactat atattcttaa agtctattca tacactatca atcaaaaact atattggaaa
     2641 tactgtttca atatcagtaa taacttattc tcattaacag gtacttttaa aaactaactc
     2701 tttgtcaagc ttcttacatg tatcatttta ataatatgtt ttatcatgta aaacaaaatt
     2761 aataaaaaca gaatttataa actactaacg atttcgatgc gtataacaac atacaaccaa
     2821 gcaacagttt accactaatt cacacacaca taattcttct gaataacatt atggaaaata
     2881 tttttaaaat ggggcaaaat cattatcaga ttttgcccca tggattattt acttcttgtc
     2941 atcatctaca acttcgtaat cggcatcata gacattgccc tgagactgag aattattttg
     3001 agattcgcca ccaccttgag tttctggttc actctgagca gcatcggctc caggctgacc
     3061 tgcaccagca cccgcttgct gataaattct ttgtgcaaac tgatgagaac tctgctctac
     3121 agcagatttg gcagctttaa tagcttcaac atccgaccca gccatagcac ttttcaactt
     3181 ttcaatttct gattcgatct tcatcttttc ggattcttca accttgtcag cattgtcttt
     3241 taaaagtttc tccaatgaat agataagagt atcagattca tttttagctt cgattagttc
     3301 tttggctttt ttgtcttcag aagcatgaga ctcggcatcc ttaaccattt tatttatctc
     3361 atcttctgat aaaccacttg atgactctat tcggatcttc tgttcttttc cggttcctag
     3421 atcttttgca gatacgtgaa caattccgtt ggcgtcaata tcaaaagata cctcaatctg
     3481 tggaactcca cgaggcgcag gaggtatatc agtaagctca aatcgcccca aagttctgtt
     3541 ctgtgaagcg agttcgcgct caccctgaag aacatgtaca gtaaccgccg gttgattgtc
     3601 cgctgcagta gaaaaaacct gactcttctt agttggaatt gttgtgtttc gctcaataag
     3661 ctttgtcata actccaccaa gagtctctat tccaagagaa agaggtgtaa cgtcaagaag
     3721 gagaagtccc ttcacatcac cggtaaga
//
LOCUS       DAOTGZ010000255         3728 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_3064
            NODE_29262_length_3728_cov_4.565206, whole genome shotgun sequence.
ACCESSION   DAOTGZ010000255 DAOTGZ010000000
VERSION     DAOTGZ010000255.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868088
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 3728)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3728)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:22:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,785
            CDSs (total)                      :: 2,751
            Genes (coding)                    :: 2,732
            CDSs (with protein)               :: 2,732
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 12 of 19
            Pseudo Genes (internal stop)      :: 5 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3728
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_29262_length_3728_cov_4.565206"
                     /isolate="AMR_MDS_3064"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(444..917)
                     /locus_tag="P5123_12470"
     CDS             complement(444..917)
                     /locus_tag="P5123_12470"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRX17122.1"
                     /translation="MKKQINTIAKINELLFFSCLCPFPVFISVVVMITGRVSLTVMQI
                     LYVGFAARGIAFMFLFFSVCTLYDLYVKITKIFTNNQKKTYINYIKHSFFPYLLFFNI
                     TILPFIYIISINNGYNWIVLFVIFCIVICLVSQIFFNVNYLKLLKSESVTRQYLE"
     gene            complement(917..1882)
                     /locus_tag="P5123_12475"
     CDS             complement(917..1882)
                     /locus_tag="P5123_12475"
                     /inference="COORDINATES: protein motif:HMM:TIGR03696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RHS repeat-associated core domain-containing
                     protein"
                     /protein_id="HRX17123.1"
                     /translation="MSSDFAYSEYFPYGEVWVNERSEIDDGNLPYKYTGKEYDPETKL
                     TYYGARYYDAKLSRWISVDPILGSYLSGKPAGGVYNSINLNLYHYAGLNPIKYIDPTG
                     MFKDESECISSSKAGLQQKEYFSKGTVTEGDSLWGIARYQLLKESNGRASISNNFNAN
                     IMARVSYIRKQNQLMSDKLMPGQKLDTGYRQNIISEGAVDSWGSHDIPLLAGALAAKG
                     AVAYGLGAAGKAIMTTEIGLKASFTAGMAFQLAKSRAGDAIVRGTNFLLQHPVEVNQF
                     ISSLISGAGPPSSSVPASYSEIFGEITGRAATAMYPPLQETMQGR"
     gene            complement(2254..2727)
                     /locus_tag="P5123_12480"
     CDS             complement(2254..2727)
                     /locus_tag="P5123_12480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010662612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium-dependent phosphatase-1"
                     /protein_id="HRX17124.1"
                     /translation="MHLIVFDLDFTLWDAAGTWCDHTCPPFQKKGDVVVDCRGGIISL
                     YPDVKKILILLKEQGYRLAIASRTGEPEWASQLMDIFDIKKYFDYLEIYPASKLVHFD
                     KMKRKLGIGYDRMIFFDDEIRNIDEVGALGVATCHVTGGITFRLVERKLMDCLRV"
     regulatory      2914..3062
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF01482"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="The adenosylcobalamin (AdoCbl) riboswitch is found
                     in the 5'-untranslated regions of vitamin B12 (Cobalamin)
                     related genes in bacteria; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="adenosylcobalamin"
                     /db_xref="RFAM:RF01482"
     gene            3063..>3728
                     /locus_tag="P5123_12485"
     CDS             3063..>3728
                     /locus_tag="P5123_12485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011878090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-methyltetrahydropteroyltriglutamate--
                     homocysteine S-methyltransferase"
                     /protein_id="HRX17125.1"
                     /translation="MDKRTLVTGFSRIGENRELKIALENYWAQKIPLPELLSIAKNLR
                     KKHWLEQKERGIDLISSNDFSLYDLMLDTTVMLNAVPDRFKEIQDDTERYFAMARGCK
                     NCTAMEMTKWFNTNYHYIVPELNSKIDFKLNANKVISEYNEAKKLGINTKINLIGPIT
                     YLSLAKSENGDNGLDYIDNILPVYAQLMDQISQLDNQVYVQLDEPIFVNNPSKECLSL
                     IGKV"
BASE COUNT         1254 a          725 c          610 g         1139 t
ORIGIN      
        1 accgaagagc tttttaaaca catagtctga ttttaaactt aaaaatgaca tgagttaatt
       61 atgggtgcta ggtataaaat gtcaatcaga aagtcttaga cagtaaaatc atatttttaa
      121 aaatattttt aataaaacgt ctattaatcc ggatccttac caaaatgtac tgaacctggc
      181 taacaatcca ggaaaacgca gtttaaagtc attagtgttg ttagatgatc agcagatgtg
      241 gtttcgtcca atacagtctt agcgcagctg ttatccgcca ttctttcatc gtgattcttg
      301 taagttattt cggctgatat tcaatcagtt ttttttacga cagctgcgcc aagacttcta
      361 aacataatat tgatgatgct gactatcaat aatctgtccc aaaaaaaata cattagccga
      421 gcagaaaaaa aagttttcaa aaattattca agatattgtc tagtcacact ttcagacttc
      481 aataatttta aataattcac attaaaaaat atttgggaaa caagacaaat aacaatgcaa
      541 aatataacaa atagaacaat ccaattatat ccattgttta ttgaaataat atatataaat
      601 ggcaaaattg ttatattaaa aaatagcaaa tagggaaaaa aggagtgctt aatgtaattg
      661 atatatgttt ttttttgatt attagtaaat atttttgtaa tctttacata taaatcatac
      721 aaagtacaaa cagaaaaaaa taagaacata aaagcaattc cacgtgctgc aaatcctaca
      781 tataatattt gcatgacagt aagactaaca cgccctgtaa tcataacaac aacgctaata
      841 aaaacaggga aagggcacaa acatgagaaa aataacaatt cattaatttt tgcaatagta
      901 tttatttgtt ttttcattat cgtccctgca tagtttcctg aagaggagga tacatggctg
      961 ttgccgcgcg acctgttatt tccccaaaaa tttctgaata acttgctggt acgctgcttg
     1021 aaggtggccc cgcacctgaa attaaacttg aaataaactg attcacctca actggatgtt
     1081 gtagtaaaaa atttgttcct cttactattg catcaccggc acgagattta gctaattgaa
     1141 aagccatacc tgcagtaaaa gacgccttga gaccaatttc tgtggtcata atagctttac
     1201 ctgctgcgcc caagccataa gcaactgcgc ctttagcagc taatgcgcca gctaacaatg
     1261 gtatatcgtg agatccccaa ctatctacag caccttcaga aataatattt tgtctataac
     1321 cggtatcaag tttttgacca ggcatcaact tatcactcat aagttgattt tgttttctta
     1381 tgtaggaaac ccttgccata atattagcat tgaagttatt gcttatactc gctcttccat
     1441 tgctttcttt taacagttgg tatctagcaa tgccccaaag agaatcacct tccgttactg
     1501 ttcctttact aaaatattcc ttttgttgaa gtccagcttt tgaagacgaa atgcattcag
     1561 attcgtcttt aaacattccg gtaggatcaa tatatttaat aggattcaag ccagcatagt
     1621 gatacaaatt aagatttatc gagttgtaaa caccaccagc aggttttcct gacaggtaac
     1681 taccaagtat aggatcaaca gagatccacc tcgacaactt cgcgtcgtag tagcgcgctc
     1741 cataatatgt taatttagtt tcgggatcgt attctttgcc ggtgtatttg tacggtaagt
     1801 taccatcgtc tatttcggag cgttcgttga cccagacttc gccatatggg aagtactctg
     1861 agtaggcaaa atctgacgaa ataagccctt tttagggctc tttttcaggg aatttgcgtt
     1921 aaaaaactcg ggcttcgcga tccggtgaca caaaaaattt caaagatcag ctttacaatg
     1981 ctttataagc tctcaattat atggttaatt ttacatatca tcagcattaa gtcaagcctt
     2041 ttcatatatg ccgcggtttt ccgctaaacg accagtctcc atgttcagat ttttatccca
     2101 agggtgatac tatttcattg tttttcgcaa tttgtgagtt tgcaatggct ctgcctattt
     2161 agcacatatt tatttataat taatttaaca tcaaatgcat attatcattg aaaaacgcta
     2221 tcatatttat cctgtgatct ataattatga tccctaaacg cgtaaacagt ccatcaactt
     2281 tctctcaacc aaccggaagg taattccacc tgttacgtga cacgtcgcaa caccgagagc
     2341 tccgacctca tcgatgtttc ttatctcgtc atcaaagaag atcatccggt catagcctat
     2401 acccaatttt cttttcattt tgtcaaaatg aaccagtttt gatgcaggat atatttcaag
     2461 ataatcaaaa tattttttta tatcaaaaat atccatcaac tgcgatgccc actccggctc
     2521 tccagttcgc gaagcaatag caagtctgta accctgctcc tttaacaata tgagtatttt
     2581 ttttacatca ggataaaggg aaataattcc gccccggcaa tcaacaacaa catcgccttt
     2641 cttttgaaat ggcggacagg tatggtcaca ccaagttccg gcggcatccc aaagagtaaa
     2701 atcaagatca aatacaatta agtgcaatag taaatacctc tttttgttat tatttcccat
     2761 taatttaaaa aaataatata agtcaaaaat taaaaaaata attcgctatt ttaggtttaa
     2821 gaagtgctca gtactctaaa ataagccgat aaagcaacaa gatcaattga tcttaaaaca
     2881 ttatcgttga catttacaag gtgttctttt gtttaaggta aagtattgtt gccgtttatg
     2941 gggaagtcct gtgagaatca ggcacagtcg cgctacggta ttgagaataa cattatttct
     3001 ctaagtccga ttaccatggc agcatataac gaattttgtc gcgcaaacaa aaggagatta
     3061 aaatggacaa aagaacactt gtcaccggtt tttcaagaat cggtgaaaac cgagagctaa
     3121 aaatagctct tgaaaactac tgggcacaaa aaatacccct gcccgagctt ctaagcatcg
     3181 cgaaaaacct gcgaaaaaaa cactggctcg aacaaaaaga gagaggcata gacttaatta
     3241 gctctaacga ttttagcctt tatgacttga tgctcgacac aacagtaatg ttaaatgctg
     3301 tacccgaccg atttaaagag attcaagacg atacggaacg ttactttgcc atggccagag
     3361 gctgtaaaaa ctgtacagca atggaaatga caaaatggtt taacaccaac tatcactata
     3421 ttgttccgga acttaacagc aaaattgatt tcaaacttaa cgctaacaag gttatttctg
     3481 aatacaatga agcaaaaaag ctaggtataa acacaaagat caatcttatc ggtccaatca
     3541 cctatctctc tcttgcaaaa tctgagaatg gagataacgg tcttgattat attgataata
     3601 ttttacccgt ttatgcccag ctgatggatc agatatcaca gcttgacaac caggtctacg
     3661 ttcagcttga tgagcctatt tttgtaaata atccctcaaa agaatgcctt tcactaatcg
     3721 gcaaggtt
//
LOCUS       DAOTGZ010000256         3719 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_3064
            NODE_29352_length_3719_cov_4.254094, whole genome shotgun sequence.
ACCESSION   DAOTGZ010000256 DAOTGZ010000000
VERSION     DAOTGZ010000256.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868088
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 3719)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3719)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:22:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,785
            CDSs (total)                      :: 2,751
            Genes (coding)                    :: 2,732
            CDSs (with protein)               :: 2,732
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 12 of 19
            Pseudo Genes (internal stop)      :: 5 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3719
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_29352_length_3719_cov_4.254094"
                     /isolate="AMR_MDS_3064"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1785
                     /locus_tag="P5123_12490"
     CDS             <1..1785
                     /locus_tag="P5123_12490"
                     /inference="COORDINATES: protein motif:HMM:NF015983.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HRX17126.1"
                     /translation="ASLKTQLDSHVDIAKLLDVQLCDYYAHNSADYIADDLCADAFKM
                     VGFSTYLWNRELVEKICKIIKTKLPETILFAGGAEATALPLSLLESAPFDFVIKGEGE
                     IVLREVMKSLLGNKALDDIAGLFMRGGKHNPDKDQQPVLDLNSLDSPFLSGSLDPAEY
                     SGLLWELSRGCPFKCGFCFESRGVAGVRKFSMDRIKRELELFEEKKVSQVFVLDPTFN
                     SDAARAKLILRMIHKITPLIHFNFEVRTEFLDSEIASLFAEINCTLQIGLQSAHSDVL
                     ASVNRTFNAEAFADKISLLNKEGVLFGLDLIFGLPGDTLNGFKESLDYALALQPNHLD
                     IFRLTVLPGTALYDQAGSLDLNALKDAPYTLVSSSTFSESELEQAESLKLACDLFYNR
                     GGAVGWLFMVLEELELNASDFFSGFVEYMVVQKDPDLLTSDEICELQLSFVKKMFLKE
                     NKDDFFSVIEDIIKINNALNKSLYAGPLVNTDAGIHDDKSIYKLAPGTVTLFLKYDYN
                     DLMSVGELNFEEFLDNYDQQTTCLIIYNCNGIVKSLIITENLITILNSLDGRLSIKNI
                     CDNESEDNCIEIREFLDYAVAEQIVQMC"
     assembly_gap    1887..1986
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            2269..2904
                     /locus_tag="P5123_12495"
     CDS             2269..2904
                     /locus_tag="P5123_12495"
                     /inference="COORDINATES: protein motif:HMM:NF013921.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - transposition, DNA-mediated
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HRX17127.1"
                     /translation="MRKPREIVEGSSYHVTARINRGEYLLQDNFEFDLKKMFIEVVHR
                     THQIYNFRIYVIEVMDNHIHMMIKPEGNSELGNIMNSILSTFARRYNILFKCHGHVWY
                     DRFKSKVIKNTQQLINTIRYICNNPYRAGMVNNPLNYKYSSVYSIYAKIKTELVSLIL
                     SIMDLSDKNIRSIYDDYRKEFSFEKASAVNKLLGFYPNRSGRPKKIAICGE"
     gene            complement(3288..>3719)
                     /locus_tag="P5123_12500"
     CDS             complement(3288..>3719)
                     /locus_tag="P5123_12500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012800782.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YaiI/YqxD family protein"
                     /protein_id="HRX17128.1"
                     /translation="DACPTVIKDILLRAAERKKIPIIFVANCALKLPDSVFVSMHVVE
                     KGPDIADDWIANNANSGDLVISEDIPLADRVIEHGAVVLSTRGVFLDKANIKARLATR
                     DLLSDLRDSGMETGGPPPFDQRTVRTFANGLDRFLQKKCIN"
BASE COUNT         1112 a          631 c          753 g         1123 t
ORIGIN      
        1 gcttcattaa agactcagct ggattctcat gtggacattg cgaaactgct tgatgttcag
       61 ttatgcgatt actacgcgca taacagcgcg gattatattg ccgatgatct ttgcgcggat
      121 gccttcaaaa tggtgggttt ttctacatat ctatggaaca gggaacttgt cgaaaagatc
      181 tgcaaaatta ttaaaacaaa attaccagag acaattttat tcgcgggcgg tgcagaggcg
      241 acagcgcttc ctttgtctct tttggaaagt gctccctttg attttgttat aaagggagag
      301 ggggagattg ttcttcggga agtgatgaaa agccttctgg ggaataaggc tcttgatgat
      361 attgccgggc tgtttatgag aggcggcaaa cataatcccg ataaagatca gcagccggtt
      421 ctcgatttga actctctgga ttcgcctttt ctttcgggaa gtcttgaccc ggcagagtat
      481 agcggactct tatgggaact ttcgcggggt tgtcccttta aatgcggttt ctgcttcgaa
      541 tcaagaggtg ttgccggtgt aagaaagttc tctatggatc gaattaagag ggagcttgag
      601 cttttcgaag agaaaaaagt gtctcaggtt tttgtgctgg atcccacctt taacagcgat
      661 gccgcgcgcg caaaactgat actgcggatg atacataaaa ttactccctt aattcatttc
      721 aattttgaag tacgaaccga gtttctggat agcgagatcg cctcgctgtt tgctgaaatc
      781 aactgcacac tgcagatagg gctgcaaagc gctcattctg atgtgttagc aagtgtaaac
      841 agaacattta acgcggaagc gtttgccgat aaaatatcac tgttaaataa agagggtgta
      901 ctgttcgggc ttgatcttat ttttggtctt cccggtgaca ctctgaatgg gtttaaagag
      961 agtcttgatt acgcgcttgc tctgcagccg aatcatctgg atattttccg actaacagtt
     1021 ttgccgggaa ccgctctcta tgatcaggcc ggttcgctgg atttgaatgc cttaaaagat
     1081 gctccatata ctcttgtatc ttcttcgact ttttctgaat cagagcttga acaagccgaa
     1141 tcattaaagc tggcctgcga ccttttttat aaccggggcg gtgccgtggg atggcttttt
     1201 atggttctgg aagaactgga acttaacgcg tcagattttt tttcagggtt tgtagaatat
     1261 atggttgtgc aaaaagatcc cgatttatta acaagtgacg aaatttgcga gttgcagttg
     1321 tcctttgtaa agaagatgtt tttaaaagag aataaggatg atttcttttc tgtaatcgaa
     1381 gatattatca aaataaataa tgccttaaac aagtcgctct atgccggccc acttgttaac
     1441 actgatgccg gtatacacga tgataagtca atatacaaat tggcacctgg gaccgtgact
     1501 ctttttttaa aatatgatta taatgatctt atgagtgtag gtgaactaaa ttttgaagaa
     1561 ttcttggata attatgacca gcaaacaact tgtctgatta tttataattg taatggaatt
     1621 gtaaaaagtt taataataac cgaaaatctt attactattc taaattcatt ggatggtcgt
     1681 ctctctatta agaatatatg cgataacgag agtgaggaca attgtattga aataagggag
     1741 tttcttgatt atgcagtagc cgagcaaatt gtacagatgt gctgaaaaat gggataaata
     1801 gggtcggtgg tcgtgtgatc atggcacacg gaaatgggga cggtggtcaa accaaaaatg
     1861 gggacggtgg tcatatgatc tgataannnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1921 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1981 nnnnnnaaat ggggacggtg gtcaaaccaa aaatggggac ggtggtcata tgatctgata
     2041 acgggaaatg gggacggtgg ttaaaaagaa taacaaaaat aaaaaaaatt ttaacaaaat
     2101 ttgatttaat cagttataca tattagatac taatattctt ttttgataag gacaagtgtt
     2161 gaaacttgtc ctttgtattt attaaatatc ctgctaattc attttacaga taggtataag
     2221 gataaaataa ttataatctg actaatattt tgtattgagg ggaattctat gagaaaaccg
     2281 agggaaatag ttgaaggctc ttcttatcac gtaactgcac gtattaatcg aggcgaatat
     2341 ttattgcagg ataattttga atttgattta aagaaaatgt tcattgaggt tgtacatcga
     2401 acacatcaga tttataattt tagaatatat gtgattgaag ttatggataa tcatatccat
     2461 atgatgatta aaccggaagg aaattcagaa ttagggaata ttatgaactc aattttgagt
     2521 acttttgcgc gtcgatataa tatattgttt aaatgtcatg gtcatgtctg gtatgatcgc
     2581 tttaagagta aagtgataaa aaatacacaa cagctaataa atacaattcg ctatatttgc
     2641 aataatccat atcgtgccgg tatggttaat aatccgttaa attacaaata tagttcagta
     2701 tattcaatat atgcaaaaat taagacagaa cttgtttcgc ttatattaag tataatggat
     2761 ttatcggata aaaatattcg ttcaatctac gatgattata gaaaagaatt tagctttgaa
     2821 aaggcatccg ctgtaaataa gttattaggg ttttatccaa atagatctgg acgtcctaag
     2881 aaaattgcaa tttgtggtga atgatgatat agtttctcag actgcattta gtttcaataa
     2941 tgaatttaaa gatatatata acaaatcgag agcaccatgt tttgttgtac tacgagccag
     3001 agtttaattt tatggatact ctcttactcc tcataataat tattaatcaa cccctttgat
     3061 ccctttgaag aaacattatt ttactaaaat agatagagaa tttttttaat attcttattc
     3121 agaatacaaa tacataatat ccatcgtccc cataatgaac aaacaccgtc cccaaaggca
     3181 aattgctgtc aaacaaccac cgtccccatt tgcccaataa cacatcggtc tgctgttaaa
     3241 ataaccaccg tccctaaaat tcccagaata accaccgtcc ccaaatacta atttatgcat
     3301 tttttttgca aaaaccggtc aagcccgttt gcaaaagtac gcactgttct ctgatcaaag
     3361 gggggcggac cgccagtttc catgcccgag tcacgcaagt ctgaaagcag gtctcttgta
     3421 gcaagccgtg ccttgatatt cgccttatcc aggaatactc ctcttgtgga aagaacaact
     3481 gcaccatgtt ctatcaccct gtccgcaagc gggatatctt cgctgataac aaggtcgccg
     3541 ctattcgcat tattagcaat ccagtcgtcg gcaatgtccg gtcccttttc aaccacatgc
     3601 atcgaaacaa agacagagtc cggcagtttt agtgcacaat tagcaacaaa tattataggg
     3661 attttctttc gctctgcggc acgtagcagt atatctttaa ttactgttgg gcatgcgtc
//
LOCUS       DAOTGZ010000257         3708 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_3064
            NODE_29470_length_3708_cov_4.575691, whole genome shotgun sequence.
ACCESSION   DAOTGZ010000257 DAOTGZ010000000
VERSION     DAOTGZ010000257.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868088
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 3708)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3708)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:22:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,785
            CDSs (total)                      :: 2,751
            Genes (coding)                    :: 2,732
            CDSs (with protein)               :: 2,732
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 12 of 19
            Pseudo Genes (internal stop)      :: 5 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3708
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_29470_length_3708_cov_4.575691"
                     /isolate="AMR_MDS_3064"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1635)
                     /locus_tag="P5123_12505"
     CDS             complement(<1..1635)
                     /locus_tag="P5123_12505"
                     /inference="COORDINATES: protein motif:HMM:NF024779.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4105 domain-containing protein"
                     /protein_id="HRX17129.1"
                     /translation="MLFRSFFLLLVLSFFAGWGAPLYGEELVNKAVSAKLYNDPYWHT
                     LLHYRFYGYSDGKPVYSSDVKSRSFFLSENGKNDPKAEMTASIQQILIEKKIDIINRF
                     PARSRFLAGKLAPSTYNLDNFLDHNYKHLISTLDDFDSIKLVYTGEYMGNPASFFGHT
                     FLRIDHKNEGALLGTALNYAARVNSEGSIAYIVKGVTGGFDGYYSVNSYFSVVRDYSY
                     RQSRNIWEYSLNIDRQEYRRLLEHLIELSDISSEYYFFDRNCSYMLLYLLEAARPGVT
                     LVDKTGFFVMPVETVKLVEESGLIEETVFRPSIGERLYNRTRKMRTDDVKRAVNIALQ
                     IESTLEVVGDDDLSDSRKASILEFSLDYYRFLEFPRLTEEESDQFNRKIMRLNKTRSL
                     VKHSNEELPLVKSDPLLSHGPFRLSSGCGRSDNEGNFIYMGIRPAVHSLTDPPAGYRF
                     GSSVTALDTEFRIDYERPSTLYVQSIKLLEIVSLEPLTAYSSPLSYRLYTGVIRGKWE
                     DEIYASSISVETGFSLQPVQKLLLFAMPGFTAGYSGG"
     gene            2069..3595
                     /locus_tag="P5123_12510"
     CDS             2069..3595
                     /locus_tag="P5123_12510"
                     /inference="COORDINATES: protein motif:HMM:NF012245.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein"
                     /protein_id="HRX17130.1"
                     /translation="MGLWNNISLKFKILLPFVLMLAIIVSYFVFFFLPDMEDSMYNEK
                     KLMVQNIANAAVNQIAAIDKRHRNGEFTKEEAQEHAKDIIRNMRYGEDGYDYVWINDF
                     EPRMVMHPTTTDLNDRNLSDYEDPTGHKFFQTIVKIAQSEEGGFFEYHWKAKKGDAIV
                     PKYSFVKPYKPWGWIIGTGIYVDEVKAQFEELLQKVLIILGVIAVVFIAIIILITYVI
                     TSPIIENVKVAERISNGDLTSKMRKHGKDEIGRLSDAMDKMQLEVSNVIQSILNSTQI
                     LTSSSEEINQTAIRLSEAANEQAANMEEISSTMEEIGSAITQNSQNAKETDRIAHESA
                     QKTKEGEKAVEETVDAMNQIAEKIHIVEDIAYQTNLLALNAAIEAARAGEHGKGFAVV
                     ASEVRKLAEKSQVAAVEIRDLAVNSVTVANQAGALLQAIVPEITKTADLVQNITIASE
                     EQDSGATQINTGMNQLSQAAQDTAASSEELASTSDLLKDHAVRLNKTVEYFKIKNNEE
                     "
BASE COUNT         1213 a          765 c          753 g          977 t
ORIGIN      
        1 tccaccgcta tagcccgctg tgaatccagg catcgcgaaa agaagcagtt tttgtaccgg
       61 ctgcaggcta aagccagtct ctacagagat agaagatgcg taaatttcat cttcccattt
      121 tcccctgatt actcctgtat ataatcggta agacagtgga ctggaatagg cagtaagtgg
      181 ctctagagaa acaatttcca gtaatttgat actttgtaca taaagcgtgc taggtctctc
      241 ataatcaatt cgaaattcag tgtccaaggc tgtcactgac gagccaaacc tgtaaccagc
      301 tggaggatcg gtcagcgaat gaacggcagg ccggataccc atatagataa aattgccttc
      361 attatcggat ctgccacaac ccgaggacaa tctgaaaggc ccgtgtgaca ataacggatc
      421 acttttaacc aatgggagct cttcattaga gtgttttacc aaacttcgag tcttattaag
      481 ccgcataatt ttacgattga actggtcaga ttcttcttct gttaaacggg gaaattcaag
      541 aaaacggtaa taatcaagtg aaaattcgag aatcgaagcc ttcctactat cgcttaaatc
      601 atcatctcct acaacctcca aagtgctctc tatttgcaag gctatattta cagccctttt
      661 aacatcatcg gttcgcattt tcctggttct attgtacaga cgctccccga ttgaaggacg
      721 gaaaaccgtt tcctctatta atccggattc ctcgaccagt ttgacggttt cgacaggcat
      781 tacaaaaaat ccggttttat cgacaagagt aactcctgga cgggccgcct caagcagata
      841 tagcagcata taagagcaat tgcgatcaaa gaagtagtat tccgatgaga tgtccgaaag
      901 ttcaatcaga tgctccagaa gacgtcggta ctcctgccga tctatattca gagaatactc
      961 ccatatattc cgggattgcc tgtaagagta gtcacgtact actgaaaaat aactattaac
     1021 tgaatagtat ccatcgaaac cgcctgtaac acctttaact atataggcta ttgagccttc
     1081 gctgttaacc cttgctgcat agtttaacgc agtccccaaa agggcaccct cgttcttgtg
     1141 atcaattctt aaaaaagtat gaccaaagaa cgaagcagga ttacccatat attcacctgt
     1201 ataaacaagc ttaatcgaat cgaaatcatc gagagtgctt atcagatgtt tgtagttatg
     1261 atccagaaaa ttatctaaat tataagttga gggagcaagc ttgcctgcaa gaaaccgact
     1321 tcgagccgga aaacgattta ttatatcaat ttttttttct atcagaatct gctgtatcga
     1381 agctgtcatt tcggcttttg ggtcgttctt tccattttct gaaaggaaaa aacttctgga
     1441 ttttacatca ctactgtaca ccggctttcc atccgaataa ccgtaaaatc ggtaatgaag
     1501 cagggtatgc cagtatggat cgttatataa ctttgcggag acagctttat tcacaagttc
     1561 ttcgccatac aggggagccc cccagccggc gaaaaaagac aaaactaata ataaaaaaaa
     1621 agatctaaaa agcatacaca tcctcaaatg ggatttcggt gaaccattat cacagagtca
     1681 cagagacaat taccgaattt ttctctcaaa agaacagact ttaacataac atctgctcat
     1741 gtaatgtttg tgtaaatttt aggtaaccta taaaatattg ctaatttttc gaagttttcg
     1801 taataaatcg aaaccgtaac aattcggtga caaaataatg ctttttaaaa catttttatc
     1861 cctttttatt tggaaaactt accgagtatg aatatcttgt caattagcaa aacacggaac
     1921 attgaaacct cataatgaaa tatgattaac aatttaatta accatatttt cctctggtta
     1981 acaaaccatt tttcatgaaa ctttatgagg gcttgtcgga aagcacttga gatgtatccg
     2041 aaaaattata attataaagg ttattgcaat gggactatgg aacaatatct ctcttaaatt
     2101 caagatttta ttaccctttg tgctgatgtt agccataatt gtttcgtatt tcgtcttctt
     2161 ttttctgcca gacatggaag atagcatgta caatgaaaaa aaactaatgg tgcagaacat
     2221 agccaatgcg gcggtcaacc agattgcggc tatcgacaaa cgccaccgaa acggagaatt
     2281 caccaaggaa gaggctcaag aacatgccaa agatataatt cgaaatatgc gctacggtga
     2341 agacggctat gattacgtat ggattaacga ctttgagcca cgaatggtaa tgcatccgac
     2401 cacaacagac cttaacgacc ggaacctttc tgattatgag gacccaaccg gacataaatt
     2461 ttttcagacc atcgttaaaa tagctcaaag tgaggaaggc ggatttttcg aataccactg
     2521 gaaggcaaaa aaaggtgacg caattgtacc taaatactcc tttgtaaagc cttataaacc
     2581 ttggggatgg ataatcggaa caggaatcta tgtggacgag gtgaaagcac agtttgaaga
     2641 gctgctgcaa aaagttctga taatacttgg agttattgca gttgtcttta tagcaataat
     2701 aatccttata acatatgtaa tcacaagccc gattatcgaa aacgttaagg ttgccgaaag
     2761 aatctcaaac ggtgatttga cctctaaaat gcgtaagcac ggcaaagacg agattggtcg
     2821 cttatccgat gcaatggaca agatgcagct tgaggtctct aacgttatac agagtattct
     2881 taacagcaca cagatactga cctcttcatc cgaggagata aaccagactg caataaggtt
     2941 aagtgaagcc gcaaacgaac aggccgcaaa catggaggag atatcttcga caatggagga
     3001 gataggttct gctattaccc aaaactcaca gaacgccaag gagacagatc gcatcgctca
     3061 tgagtcggct caaaaaacca aagagggcga aaaagcggta gaagagactg ttgacgcgat
     3121 gaatcagata gctgaaaaga tacatattgt agaagatatc gcgtaccaga caaacctgct
     3181 cgcacttaac gcggcaattg aagctgcacg cgcaggtgag catggcaagg gttttgcagt
     3241 tgttgcgagt gaagtaagaa agcttgcaga gaagagtcag gtcgcggcgg tagagataag
     3301 ggatctagct gttaactctg taaccgtagc aaatcaggca ggcgcactgc ttcaggcaat
     3361 agttcccgaa attaccaaaa cagccgatct tgtacagaat ataaccatag cctctgaaga
     3421 acaggactcg ggagcaaccc agattaacac cggaatgaac cagttgagtc aggcggcaca
     3481 agatacagca gcctcatccg aagagctggc atcgacttcg gatcttctga aggatcacgc
     3541 tgtgaggctt aacaagacag tagaatattt taaaatcaaa aataacgaag agtaatatcc
     3601 gaaagccgtc cactttgggc ggctttttta aaccttgagg aaatttcctc gtattgttcc
     3661 aaaaaataag ctactggtaa tctttgataa ttaacttttt aacaggga
//
LOCUS       DAOTGZ010000258         3706 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_3064
            NODE_29495_length_3706_cov_4.367023, whole genome shotgun sequence.
ACCESSION   DAOTGZ010000258 DAOTGZ010000000
VERSION     DAOTGZ010000258.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868088
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 3706)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3706)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:22:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,785
            CDSs (total)                      :: 2,751
            Genes (coding)                    :: 2,732
            CDSs (with protein)               :: 2,732
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 12 of 19
            Pseudo Genes (internal stop)      :: 5 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3706
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_29495_length_3706_cov_4.367023"
                     /isolate="AMR_MDS_3064"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..546
                     /locus_tag="P5123_12515"
     CDS             <1..546
                     /locus_tag="P5123_12515"
                     /inference="COORDINATES: protein motif:HMM:NF039580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRX17131.1"
                     /translation="YRSSDRVLNRDVLIESYTEIMDLLKYEYVLPQEIDDSTVIDKLL
                     ADFFGKRGFISMNGNNLEITSNDNLRSDLMLLATGIEDILESCFCAAHSILKIKNKTV
                     ESRAFTAMLAEHGKALYLTGKISLVESVSVPVFRNLLKLLTEKKIIEEKEISKRKNGI
                     EIIDKDKAELLRKEVAALMVM"
     gene            complement(556..1143)
                     /locus_tag="P5123_12520"
     CDS             complement(556..1143)
                     /locus_tag="P5123_12520"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRX17132.1"
                     /translation="MKKIQGIIIILACLLVFMPGQSFALINMEVFTGSSFEGRVAGLE
                     AEDDVSGMNYGFRFNALYRIPVFDFGLGGFMQGSPLTYEMKGETYKLNKLTLGIDSFA
                     RFKPEIAPVYPYIRAGLAIYDKSETTMVNSDDISITEFNFRASYYGLGVSYPLVPMPV
                     IDVHLFMEYLYDISKVAKDNELRCHKLNIGLFMSI"
     gene            complement(1225..3627)
                     /locus_tag="P5123_12525"
     CDS             complement(1225..3627)
                     /locus_tag="P5123_12525"
                     /inference="COORDINATES: protein motif:HMM:NF012700.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Smr/MutS family protein"
                     /protein_id="HRX17133.1"
                     /translation="MKTITEHELNLLDWKEVTAIYQKRCMTSSGRDLYNNLEELEISE
                     AKLCTTRIDEIRDSIINGLSMPLTPIFNIIKPVMLAYKGSVLCLEEIYQISRTVKTIN
                     EFCCFLDDNKKDFASLMPLRDTLTALPIIEKELTSAITDDVLLNETKYPQIGKLHREI
                     DSIKNEIKSRLQQLVNSPEFSDHVQERIVTTAGNKYVILLKSSSRGKIPGTIIDTSSS
                     GATFYFEPKAASELNNRLLSRENDLNRELFTIMKSLSETAGSCADVIGENLKIIAYMD
                     FLHAAAKTAIDHDFNSVSYTNRPELNLINAAHPLLYTESPVNTVRNTISLGKDHRGII
                     ITGANTGGKTILLKTTAILILLARCGLPIPASADSIIGKYETICVDIGDDQNLQKSLS
                     TFSGQIVSLNSMIKKCDSSSLLIIDEIISGTNPRHAASLAMAVLEFFELRGVPVIIST
                     HYPELKEFASKSNYYTNASVSFDVDSLSPTYRLNIGTPGTSYAFEIARIYQLEESIID
                     RATRYLSENEIISDQTIEKVNRLEHELIEKKQQAEKLEKSLNKQRENYYELNEKLRQK
                     IENIKEEASIDYLKQLETWKSELATRISSIQQLSMKESQQLIETISEQSKTVIKNSHK
                     FKEKRATQDYHDFDPESAQIGSQVFIIPLEKKAKLLEINEQKREVVVALGASIKSRFS
                     FDKIKLCDTNTIFETSKPVCIQTNAQQAKSDETILTIQNEHNSVDLRGMRASDAIMLT
                     EQRLDSMLKSGIPAAVIIHGHGTGALKNAIRDYLSSCSFVTLYRPGGSNEGGDGVTIA
                     YL"
BASE COUNT         1075 a          666 c          730 g         1235 t
ORIGIN      
        1 tatcgttcaa gcgacagggt gcttaaccga gatgttttga tagaatctta caccgagata
       61 atggatcttc taaaatatga atatgtattg cctcaagaga tagacgattc gactgttatt
      121 gataaacttc ttgcagattt ttttgggaaa agaggtttta tttccatgaa tgggaacaat
      181 cttgaaatta ccagcaatga taatcttcgc tctgatctca tgcttttggc aaccggtatc
      241 gaagatattc ttgaatcttg tttctgcgcc gcacattcta ttttaaaaat taaaaataaa
      301 actgtcgaat ccagagcttt tacagcaatg cttgcggagc atggaaaggc gttatatctt
      361 accggaaaaa tatctcttgt tgaatcggta tcggtgcccg ttttccgtaa cttgttaaaa
      421 cttttaacag aaaagaaaat aattgaagag aaagagatat caaaaagaaa gaatggtatc
      481 gaaatcattg ataaggataa agcagagcta cttagaaagg aagtagccgc actgatggta
      541 atgtaaattc acagattaaa tggacataaa gagtccgatg tttagtttgt ggcatcttaa
      601 ttcgttatct tttgcgactt ttgaaatatc gtaaaggtat tccataaaaa gatgaacatc
      661 tatcacaggc attgggacaa gggggtaaga tactccaagc ccatagtatg atgctcggaa
      721 attaaattcc gttatggaaa tatcatcgga atttaccatg gttgtctcgg atttgtcgta
      781 aatagccaaa cccgctctta tgtaaggata tacaggagca atttcaggtt taaatcttgc
      841 aaaagagtct attcctagtg tcaacttgtt taacttgtag gtttcaccct tcatctcata
      901 tgttagagga gatccttgca tgaatccgcc caatccgaaa tcgaaaacag gtatgcgata
      961 taaagcgtta aatcgaaaac cgtaattcat accggaaaca tcatcttctg cctcaagacc
     1021 ggcaacacga ccctcaaaag aagagccggt aaaaacttcc atattaataa gagcgaaaga
     1081 ttgacctggc ataaaaacaa gaagacaggc caaaataatt attattccct gaattttttt
     1141 catatattta tccttgatcg agacataata aatgtcaatc actaaaccca atggaagtct
     1201 tttattctaa attttcctac aaaactataa atacgcaata gttactccgt ctccgccctc
     1261 gtttgaaccg cctgggcggt ataaagttac aaaactgcag gatgaaagat aatcacgtat
     1321 cgcattttta agagctcctg taccatggcc gtgaattata acggctgcgg gtatgcctga
     1381 tttcaacatc gagtccagtc tctgctctgt cagcataatc gcatctgaag ccctcatacc
     1441 gcgcaaatca accgaattat gctcgttctg aatagtcagt attgtctcgt ccgattttgc
     1501 ttgttgagca tttgtctgta tgcacacagg tttagaagtt tcaaatatgg tatttgtatc
     1561 acataatttg attttatcaa aagaaaagcg tgattttatg gaagcaccaa gggcgacaac
     1621 cacttccctt ttctgctcat tgatttccaa tagtttcgcc tttttttcaa ggggaatgat
     1681 gaaaacttgg ctcccgatct gagcggattc aggatcgaaa tcgtgataat cctgtgttgc
     1741 ccgtttttcc ttgaacttat ggctgttctt tattacggtt ttggactgtt cgctgatcgt
     1801 ttcaattagt tgctgtgatt ctttcatgga gagctgttga atagaagata ttcttgtagc
     1861 aagttcggat ttccaggttt ctagctgttt aagataatca atggaggcct cttctttgat
     1921 gttttcgatt ttttgtcgaa gtttttcatt gagctcgtaa tagttttccc tctgtttatt
     1981 aagggatttt tctaattttt cagcctgttg tttcttttcg ataagctcat gctctaatct
     2041 gttaactttt tcgatggtct gatcagatat tatttcattc tcagatagat atcgggttgc
     2101 gcgatcaatt atggattctt caagttgata aatccgggct atttcaaacg cataagatgt
     2161 tcccggcgta ccaatattaa gtcggtaggt aggtgaaaga gagtcgacat caaaactgac
     2221 tgaggcgttt gtatagtaat tgctttttga tgcaaattct ttcagttccg ggtaatgagt
     2281 tgatattata acaggtactc cccttaattc gaagaattcc agtaccgcca tcgcaaggga
     2341 ggccgcatgt cttggatttg tacctgaaat gatttcgtct attattaaaa gtgacgagga
     2401 gtcgcatttt tttatcatgc tatttaatga cacaatttgc ccagaaaatg tcgaaagaga
     2461 tttttgcagg ttctgatcat cccctatatc tacacatata gtttcatatt tcccaataat
     2521 tgaatccgct gatgccggaa tgggtaaacc gcatcgtgcc aaaagaatta aaatagcggt
     2581 tgtttttagt aatattgttt tacctccggt atttgctccg gttataatta tccctctatg
     2641 gtctttaccc agagagattg tatttcggac tgtattgacg ggagattcag tgtaaagaag
     2701 aggatgtgca gcgtttatca gatttaattc cggtctgttt gtatacgaaa ccgaattaaa
     2761 atcatgatca atagcggttt tggctgctgc atggagaaaa tccatatagg ctatgatttt
     2821 aagattttcc cctataacat cagcgcatga accagctgtc tctgaaagac ttttcatgat
     2881 tgtaaaaagt tctctgttta gatcattttc acgacttaat aggcgattgt ttaattccga
     2941 agcggccttg ggttcaaaat agaaggtagc tccacttgag gatgtgtcaa ttattgtacc
     3001 cggtatttta cccctagaag aggactttaa aagaattacg tatttgttgc cagctgttgt
     3061 aacgattcgt tcttgtacgt gatcagaaaa ttccggagag ttgactaatt gttgaagacg
     3121 agattttatc tcgtttttaa tgctgtcgat ttctctatgt aatttaccta tttgtggata
     3181 ttttgtttca tttaacagaa cgtcatcagt aatggcgctg gtcaactctt tctcgattat
     3241 tggtaaagca gttaaagtgt cgcgtaacgg catcaggctc gcaaagtcct ttttgttatc
     3301 gtctaaaaaa cagcaaaatt cgtttattgt tttaactgta cgtgatatct gataaatttc
     3361 ttccagacaa agaacggaac ctttatatgc gagcataacg ggtttgataa tattaaaaat
     3421 aggagtcaag ggcatactaa gaccattgat aattgaatcg cgtatttcat caattcgggt
     3481 agtgcatagt tttgcctctg aaatttcgag ttcttcaaga ttattataga gatctcgtcc
     3541 cgaagaagtc atacatcttt tctgatatat ggcagtaacc tctttccagt cgagtagatt
     3601 tagctcgtgt tcggttattg ttttcatgtt gcatttatcc ttatttcagg tgttcccctg
     3661 ttgaaaaaat aataattatt gccggctttg tgtgtgcaac tgtttt
//
LOCUS       DAOTGZ010000259         3696 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_3064
            NODE_29590_length_3696_cov_5.710244, whole genome shotgun sequence.
ACCESSION   DAOTGZ010000259 DAOTGZ010000000
VERSION     DAOTGZ010000259.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868088
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 3696)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3696)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:22:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,785
            CDSs (total)                      :: 2,751
            Genes (coding)                    :: 2,732
            CDSs (with protein)               :: 2,732
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 12 of 19
            Pseudo Genes (internal stop)      :: 5 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3696
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_29590_length_3696_cov_5.710244"
                     /isolate="AMR_MDS_3064"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            26..1219
                     /locus_tag="P5123_12530"
     CDS             26..1219
                     /locus_tag="P5123_12530"
                     /EC_number="2.7.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004824138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008776 - acetate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006085 - acetyl-CoA biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetate kinase"
                     /protein_id="HRX17134.1"
                     /translation="MIILVINCGSSSIKFQLFDMPTKKDMAKGLIERIGEDDAKITYK
                     YNGDKHVEVLPIKDHKAGLAKLAQMITDDKTGVISSLSEIEAVGHRVVHGGECYNSSV
                     LIDETVIKGINECAELAPLHNPPNLMGINEAMSMFPGAKNVAVFDTAFHQTIPDYAFT
                     YPLPHKYYEDYKVRRYGFHGTSHAYVTERAAELLGKPVDQVNLITCHLGNGCSITAVK
                     NGKSVDTSMGLTPLEGLVMGTRSGDIDPAILFYLAKKENLKIEDFDRILNKESGLLGI
                     SGISNDQRNLEDLQDTNERAKLALDIFDYKLKKYIGSYMAVLNDVDAIVFTGGIGENG
                     SLTRGKSCSGLEALGVQIDDTKNEETIRGKEGEISKPSSKVKILVVPTDEEGRIASDT
                     YQLVK"
     gene            1449..2057
                     /locus_tag="P5123_12535"
     CDS             1449..2057
                     /locus_tag="P5123_12535"
                     /inference="COORDINATES: protein motif:HMM:NF012655.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HRX17135.1"
                     /translation="MDKKQKQLKKGERTKKMILMRAGTLFAQKGYHAVSIDEIIDGLG
                     IAKGTMYQYFDGKLGLLKDLLKFCRTEIDSVFESISVKDNDCRSVIREAYFKLNRLYF
                     DTFDMINLNMSSGNIIIDENYKQIAFHLMDHFVKLLSPFKEEFRIRQDEMPFVVFLQG
                     EHIRGYLRYNFGQSGAEDKDIHESIDFALDILQNGIFSNKIE"
     gene            2172..>3696
                     /locus_tag="P5123_12540"
     CDS             2172..>3696
                     /locus_tag="P5123_12540"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024629.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 39 protein"
                     /protein_id="HRX17136.1"
                     /translation="MKFRDFDNRHLTTRELSIWAILLAAGLFMLIFGITHEGIWYDES
                     YSIAAVDNTFFDLVGMIGRDSHPPLYFVMLKIFVTILGKSILTLRLFSALSIFSMGVL
                     GFTHIRKLISNRVGLIYSAMIFLTPVSVFQAQETRMYALCGAFVMIAMILLFYAINQN
                     SFKTWIAGSIAAYAAALTHYYGLIAVTILYALLLGYFIICNCDLVKRLVITCILLVVF
                     YSPVFYMLVYQTSRISKGFWIDPISLSRALSLFTYYFGYKFEMPFTVFTTIASILAVV
                     FLCLTIVSFYKNRKQTDYFAVISIILFFLTILAGLVLSAAFRPILISRYITAVFGPFL
                     FAMAYGINSLKKPELIASSVILYIIATSPVLYGIYSNNFNGPMNIVYDSIKGEVNKDD
                     IFIHGSEHTFGTFYVYFPDNKHFLYIPEDHNPYSNYEVFELNGDYGVDFNKYLDMAKT
                     RVWLTNRPGDPTNLSAVEILKHPHMKSECDVSRFNTKMGWYEVELSSFRYTHKKDPSD
                     "
BASE COUNT         1153 a          684 c          688 g         1171 t
ORIGIN      
        1 tattatacat attggagaaa ttgtaatgat aatactcgta attaattgcg gctcatcatc
       61 tattaaattt cagctttttg acatgcccac aaaaaaagat atggctaaag ggcttatcga
      121 gcgaatcggt gaagatgacg caaaaatcac ctataaatac aatggtgata aacatgttga
      181 ggttctgcct attaaagatc acaaggctgg gcttgcaaaa cttgcacaga tgattaccga
      241 cgataaaact ggtgtaattt catctttatc agagatcgag gcagtaggac accgtgttgt
      301 tcatggtgga gagtgttaca attcaagcgt cttgattgac gaaactgtaa tcaaaggtat
      361 caacgagtgc gcagagcttg ctccgcttca taacccgcca aaccttatgg ggattaatga
      421 agcgatgtca atgtttcctg gtgcgaaaaa cgtagctgtt tttgatacag cttttcatca
      481 aaccattccc gattacgcgt ttacctaccc tctccctcat aaatattatg aagattacaa
      541 agtcagaaga tacggttttc acggaacttc acacgcctat gtaaccgaaa gagcagccga
      601 acttttaggt aaaccggttg atcaggtaaa tctgataacc tgtcatcttg gcaacggatg
      661 ctcgatcact gccgtaaaaa acggcaagtc agttgatacc tctatgggac tgactccgct
      721 agaagggctc gtgatgggaa caagaagcgg tgatatagac ccagcaatac tgttttattt
      781 ggcaaaaaaa gaaaatttaa aaattgaaga ttttgaccgg atacttaaca aagaatctgg
      841 acttcttggt attagtggca tatcgaatga ccagcgtaac ctcgaggatc ttcaggacac
      901 aaacgaaagg gcaaaacttg ctctggatat ttttgactac aaacttaaaa aatatatcgg
      961 ctcttatatg gctgtactaa acgatgtaga tgctattgtc tttactggcg ggatcggaga
     1021 aaacggttca ctaaccagag gtaaatcgtg tagtggcctt gaagctctcg gagttcaaat
     1081 tgatgacacg aaaaacgaag agactattcg cggcaaagag ggtgaaattt ccaaaccttc
     1141 aagcaaggta aaaattcttg ttgtaccaac cgacgaagag ggccgtattg cgagtgatac
     1201 ctatcagctt gttaagtaat tttttagggc cggttattta aaccggccat tttttataaa
     1261 atttcactaa tcaagccgaa actaacttat ctgcaaaatc caaattttac caaactccga
     1321 cttaataatg ttgacctctg taaatctatt ttatagattc tgataattaa aacaaagggc
     1381 atttctaacg ggaacatcgt ctactttaat tgttagagac tacctataaa caacaggtct
     1441 ttttaattat ggataagaaa cagaaacagc tgaaaaaagg tgagcggacc aagaagatga
     1501 ttcttatgag ggcgggtaca cttttcgcac agaaaggata tcatgctgta tcaatagatg
     1561 agattataga tggtctggga atagctaaag gaacgatgta ccaatacttt gatggcaaac
     1621 tgggtctttt gaaagatctg ttgaaatttt gccgaacaga aattgattct gtattcgaat
     1681 ctatttctgt taaggacaac gactgtcgat ccgtaattcg agaagcatat tttaaactta
     1741 acagactcta ttttgatact ttcgacatga tcaatttaaa tatgagtagt ggtaatatca
     1801 ttatagatga gaactataaa caaattgcct tccatctgat ggatcacttc gttaaactac
     1861 tctccccatt taaagaagag tttcgtatcc gtcaagacga gatgcccttt gttgtatttt
     1921 tacaaggaga gcacattcgc ggatatcttc gttacaattt tggacagagc ggagcagaag
     1981 ataaagatat acatgaatcg atagattttg cgctcgatat tcttcaaaac ggtatatttt
     2041 caaataaaat agaataatta tctggaaatt tatgagcatt gcaaattatt tcaaaaaatc
     2101 ttaaatatct tattgacaaa actgaacttt cagttttact aattgtatct tatcatactc
     2161 ttggaggaat tatgaaattc agggattttg acaataggca cctgacaaca cgggagcttt
     2221 caatttgggc tattttactt gccgctgggt tatttatgct aatatttgga ataactcacg
     2281 aaggcatctg gtacgacgaa tcatattcta tcgcggcagt agacaatact tttttcgatc
     2341 ttgttggcat gatcggtaga gacagccatc ctccgctcta tttcgtaatg ctaaagatat
     2401 ttgttacgat attaggaaaa tcaatactga cacttcgcct tttttcggcc ctttccatct
     2461 ttagtatggg agtactcgga tttacacata taagaaaatt aatatcaaac cgtgtaggtt
     2521 taatttattc tgctatgatt tttttaacac ctgtttcggt tttccaggct caagagacaa
     2581 gaatgtacgc tttatgcgga gcatttgtca tgatagccat gatacttctt ttttacgcaa
     2641 tcaatcaaaa cagctttaaa acttggattg ccgggtctat agcagcttac gcagccgcac
     2701 ttacccacta ttacggattg attgctgtaa caatacttta tgctcttctg cttggctatt
     2761 ttattatctg caattgcgac cttgttaaac gtcttgttat aacttgcatt cttcttgttg
     2821 tgttttattc ccctgttttt tatatgctgg tttatcaaac atcaagaata tcaaaaggat
     2881 tctggataga cccaatctct ttatcaaggg cattatctct ttttacttat tatttcggtt
     2941 ataaattcga aatgcctttt acagtcttca ctacaatcgc ttctatactg gccgttgtat
     3001 ttttatgttt aaccattgtt tctttttata aaaaccgtaa acaaaccgat tattttgctg
     3061 ttatttctat aatactcttt tttcttacaa ttcttgccgg ccttgtttta agtgctgctt
     3121 tcagaccgat ccttatcagt cgctatatca cagcagtctt tggtcctttt ctatttgcaa
     3181 tggcatacgg aataaactct ttaaaaaagc cggaactcat cgccagttca gttatacttt
     3241 atattatcgc tacatcgcct gttctctacg gcatctattc taataacttt aatggaccta
     3301 tgaacatagt atacgattca ataaaaggcg aagtgaataa agatgatatt tttattcacg
     3361 gcagtgaaca tacttttgga acattttatg tctattttcc cgacaacaag cattttttgt
     3421 atattccaga agatcacaac ccttattcaa actatgaagt atttgagtta aacggagatt
     3481 atggggttga ctttaacaag tacctggata tggcaaagac tcgtgtctgg ctaacaaacc
     3541 gccccggtga cccaacaaat ctttcagcgg tcgaaatact aaaacatccg catatgaaaa
     3601 gcgaatgtga tgtttcaagg ttcaatacaa aaatgggctg gtacgaggta gagctgagtt
     3661 catttcgtta tacacataaa aaagatccat ctgatt
//
LOCUS       DAOTGZ010000260         3694 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_3064
            NODE_29604_length_3694_cov_4.856279, whole genome shotgun sequence.
ACCESSION   DAOTGZ010000260 DAOTGZ010000000
VERSION     DAOTGZ010000260.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868088
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 3694)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3694)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:22:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,785
            CDSs (total)                      :: 2,751
            Genes (coding)                    :: 2,732
            CDSs (with protein)               :: 2,732
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 12 of 19
            Pseudo Genes (internal stop)      :: 5 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3694
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_29604_length_3694_cov_4.856279"
                     /isolate="AMR_MDS_3064"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1841)
                     /locus_tag="P5123_12545"
     CDS             complement(<1..1841)
                     /locus_tag="P5123_12545"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRX17137.1"
                     /translation="MKSLLKSMFYLLIILTAVSFAGCSSGGGSSDSPEETGLVDADVP
                     SDVFAEVKGSVIELTWQGNKNDSRYMAAVTKEHEFDIKKAVKVVDIANSPQSIAGFAS
                     GRYYAWVVQVKSDGSNGKAVVANGGAALVIEDPSSNPGDDGQDDPIGDDPEPPLNAVN
                     PDIPENIEIQNPELGQVVVVWDGDSNDGTYIVVIDDDNDLNDDETVSTQEGVSSPVVI
                     DNLESDDYYVWIIPVDADGNQGEPIVVNDGQATEIFGGSLVAAPDTWEVYQGRSAIIP
                     AANLIANDGNTVNEEPLEIIEVKDAVNGTVSLEGTTITFTSTGAVGDDAGFTYVARIK
                     DNDLFKIEGSVTITVNELPAVSAEDHTKEVQQGGELYLTPASLLEGATGNDVQFVNAQ
                     NAVNGNLTVTSETITFKSTGLAGAVAQFDYKIMDNLGTEAVGTVYITVTPLPTVQAFI
                     FDNADLFEEQKITYAPPTMQNVFNSWARFDGEKYFENKDTPGISTNAAAWQLLEEPYR
                     VLMPLNVSPYNGFLSPQHLENYTFEATVTSDDKYDNDNIGLVIAFVRIDGVNYSLTAM
                     RTKGGMHPYSGWGVMYNVDEGASHDNNGNGGKIIEEKTVVSAPSGNWS"
     gene            2085..2870
                     /locus_tag="P5123_12550"
     CDS             2085..2870
                     /locus_tag="P5123_12550"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRX17138.1"
                     /translation="MKTHFIRYLLILLSLSFYIKAFSADNDQLIETAIYTFGSFEYTV
                     MTDREQNNALTDSQMYSFHKYVLKHQNVLLTQFNLSQPAKTRITICNNSKIFESETGQ
                     KAQTATFYDRAKRVLFLQEIRGLKEQNILKQTIQHKMLYLLLDTNRALSVTPGLRVLE
                     EAFCEYHFTKGRKPEVSTLPESYTTFARHLYRAIEGDDAVKQEKAFALLNLWGAHLIK
                     KIGAKAYLDILLKEREDKNELPQKHYDSFRKEYNETIANNGGI"
     gene            complement(2912..3145)
                     /locus_tag="P5123_12555"
     CDS             complement(2912..3145)
                     /locus_tag="P5123_12555"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRX17139.1"
                     /translation="MSTIINSVKKVNTAIEHIVSSSEEQKQGITQINTAITEMDTMTQ
                     QNAALVEETASASEELANQARELLDVVGQFTVN"
BASE COUNT         1145 a          807 c          645 g         1097 t
ORIGIN      
        1 gaccagtttc cactcggagc agacactaca gttttttctt ctattatttt accaccatta
       61 ccattattat cgtgacttgc accctcatca acattgtaca taacgcccca accagagtaa
      121 ggatgcatac caccctttgt tcgcattgca gtaagactgt aatttacacc atctatcctt
      181 acaaatgcta taacaaggcc gatattgtca ttatcgtatt tatcatcaga cgtaactgta
      241 gcttcaaaag tatagttctc aagatgctgt gggctcaaaa atccgttata tggcgaaaca
      301 tttagtggca ttaaaacacg atacggctct tcaagcagct gccatgcagc ggcattagtg
      361 gatatcccgg gagtatcctt attctcgaaa tatttttctc catcaaatcg tgcccatgag
      421 ttaaatacat tttgcattgt cggaggcgcg taagtaatct tttgttcttc aaatagatcc
      481 gcgttatcaa aaataaacgc ctgaaccgtt ggaagaggtg taacagttat ataaactgtt
      541 cctacagcct cggtaccaag attatccatt attttgtaat caaactgcgc aacggcgcct
      601 gccagtccgg tcgatttaaa tgttattgtc tctgaagtta cggtcagatt tccatttaca
      661 gcattttgag cattaacaaa ctgaacatca ttgcccgtag caccttccaa aagtgatgca
      721 ggtgtcaaat agagctcgcc accctgctga acctctttcg tatgatcctc ggcacttaca
      781 gctggcaatt cattaacagt tattgttact gagccttcta ttttaaacag atcattatcc
      841 ttgattcttg caacataggt aaaccctgcg tcatccccaa ctgcacctgt tgatgtaaag
      901 gtgatagtag ttccttcaag agaaacagtt ccgtttacgg catctttaac ttcaattatc
      961 tcaagcggtt cttcattaac tgtgtttcca tcgttagcaa tcaggttagc tgcagggata
     1021 atcgcgcttc gtccctggta cacttcccat gtatcaggag ccgcgacaag agacccgcca
     1081 aaaatttctg ttgcctgacc atcattaacg acaattggct caccctggtt gccatccgca
     1141 tcaacaggta tgatccaaac atagtaatca tcactttcaa gattgtctat aactacaggc
     1201 gagctgaccc cttcctgagt tgaaacagtt tcatcatcat ttagatcatt atcgtcatcg
     1261 ataacaacga tatatgttcc gtcgttagaa tcgccatccc atactacaac aacctgacca
     1321 agttccgggt tctgtatctc aatattttcc ggtatatcag gattaacagc atttaatgga
     1381 ggttcagggt catctccaat cgggtcatct tgtccatcat cacccggatt cgaagaggga
     1441 tcttctatta ctaacgctgc acctccgttc gctacaacag ctttaccgtt tgaaccatcg
     1501 ctttttacct gtacaaccca agcatagtat cgcccagatg caaatccggc aattgattga
     1561 ggtgaattcg ctatatctac aacttttaca gccttcttaa tatcaaactc atgctctttt
     1621 gtgacagcag ccatatatct gctatcattc ttgtttcctt gccaggtaag ctcgataacg
     1681 gaacccttta cctccgcgaa gacatctgat ggtacatctg catctacaag accggtttct
     1741 tcgggagaat ctgaacttcc gcctccgctg ctacaaccgg caaatgatac agctgtaaga
     1801 ataataagaa gataaaacat gctttttaat agtgatttca taattgcccc cataaagaac
     1861 ttctttctca cttattaatt agcataatac tggaaaaaaa caagcaaaaa aagtccgcac
     1921 aggtggatta attcattttt tttattttcc tgctttttcc tgcccgattt ttaaatttcg
     1981 agcaaaaaag tggctaacaa gccagcagtc atggttactt attgacaatt ttagctaaaa
     2041 caagcgagaa taagtaaatt ttttgggtca ggtccaaata catcatgaaa acacatttta
     2101 ttcgatatct attgatatta ttgtctctct ccttttatat caaggcattt tccgccgata
     2161 acgaccagct tatagagacg gcgatctata cttttggtag ttttgaatat acagtaatga
     2221 cggaccggga acagaacaac gctcttaccg atagccagat gtacagtttt cacaaatatg
     2281 ttcttaaaca tcaaaatgtt ctgttaaccc agtttaatct ttcacaacca gcgaaaacca
     2341 gaataacaat atgtaataac agtaaaatat tcgagtcaga aaccggtcaa aaagcccaga
     2401 ctgctacttt ttatgatcgc gcgaaaagag tgctctttct gcaggagata agaggcctta
     2461 aagaacagaa tatattaaaa cagaccatac agcacaagat gctgtacctt ctgcttgata
     2521 ctaaccgggc cctgtcagta accccgggac ttcgagtgct ggaagaggct ttttgcgaat
     2581 atcattttac caaaggcaga aagcccgaag tatcgacctt gcctgaaagt tacacaactt
     2641 ttgctcgcca cctttaccgg gcaattgaag gtgatgacgc cgtcaaacag gaaaaggcct
     2701 ttgcacttct taatctttgg ggagcccatc tgataaagaa aatcggagca aaggcatacc
     2761 ttgatatttt gttaaaagag agggaagata agaacgaact tccacagaaa cattacgact
     2821 ctttcagaaa agagtacaat gagacaatcg ctaacaacgg cggaatataa tattcatttt
     2881 tctgattatt cacaataaac atccggaaat atcagttaac agtaaattgt cccaccacat
     2941 caagaagctc ccgggcctga ttcgcaagct cttcacttgc cgaggcggtc tcctctacaa
     3001 gcgcagcgtt ttgctgagtc attgtatcca tttcagtaat agcagtattt atttgagtaa
     3061 taccctgttt ttgttcttca cttgaagata caatatgctc tattgccgta ttcacttttt
     3121 taaccgaatt aattatggta ctcaaagcct caccgctctg attagccttc tttgtgccaa
     3181 aacagaagta tgtatttcat tgtgacctcc gtacagctgc gcggcgaaaa gaagagcaga
     3241 ccagattgtg ataatgccaa aaaaaccagt cggcagaatg cagtatattt taaattacag
     3301 agaattgatt aaaaaatcat tttttttata cgttttaatg agtattaaaa catattctac
     3361 aatttgttca gatccctctt catagtgcat gttttctcaa ggaataatac attttagata
     3421 tccgctttta attctctacc ataaaggaat agaagcataa ataaagtcag ttaatctagt
     3481 atctgaatat aataaatatg ttgatcaaga aatgaaaaat tattcaaaaa tatttaacaa
     3541 aacagccttc aatccagtat aaatatatac aaactgtatg gaggcataaa atggaagtta
     3601 cagattttag aacatcaatc gatgttgaat taagcagact tgataggata gaagagtatg
     3661 ctgagaaaca taatatgagc atctcggatg taat
//
LOCUS       DAOTGZ010000261         3693 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_3064
            NODE_29614_length_3693_cov_3.973612, whole genome shotgun sequence.
ACCESSION   DAOTGZ010000261 DAOTGZ010000000
VERSION     DAOTGZ010000261.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868088
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 3693)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3693)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:22:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,785
            CDSs (total)                      :: 2,751
            Genes (coding)                    :: 2,732
            CDSs (with protein)               :: 2,732
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 12 of 19
            Pseudo Genes (internal stop)      :: 5 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3693
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_29614_length_3693_cov_3.973612"
                     /isolate="AMR_MDS_3064"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..256)
                     /locus_tag="P5123_12560"
     CDS             complement(<1..256)
                     /locus_tag="P5123_12560"
                     /inference="COORDINATES: protein motif:HMM:NF014654.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="segregation/condensation protein A"
                     /protein_id="HRX17140.1"
                     /translation="MNNEKDNGDIHQSAQVTSDESENTQEKYVIHIDNFEGPLDLLWN
                     LIKRSKIDIIDVSISKITEQYISYLKQMEEQNVAVASEFIA"
     gene            complement(350..712)
                     /locus_tag="P5123_12565"
     CDS             complement(350..712)
                     /locus_tag="P5123_12565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004458304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HRX17141.1"
                     /translation="MARILVVDDAKFMRTLVKDALTPKGHEIIGEAENGNVAIDMYKK
                     FKPELVTMDITMREKDGIEAAEEILKLDPNAKIIMVTALGQENLLTKAIKLGVKDFVV
                     KPFPPERLQKAAEKALNS"
     gene            complement(790..1860)
                     /locus_tag="P5123_12570"
     CDS             complement(790..1860)
                     /locus_tag="P5123_12570"
                     /EC_number="3.1.1.61"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014733664.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0008984 - protein-glutamate methylesterase
                     activity [Evidence IEA];
                     GO_function: GO:0050568 - protein-glutamine glutaminase
                     activity [Evidence IEA];
                     GO_process: GO:0006482 - protein demethylation [Evidence
                     IEA];
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA];
                     GO_process: GO:0018277 - protein deamination [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis response regulator protein-glutamate
                     methylesterase"
                     /protein_id="HRX17142.1"
                     /translation="MDKIRVLVVDDSALVRRIISDALEKDPEIEVVGTANNGKNAIFK
                     SFVLYPDVITMDIEMPVMDGLEALREIMDKNAKPVIMLSSLTQYGAEATFKALEYGAA
                     DFVPKPQAMVQMSFDEISEQLVQKVKAYAKAEINYSAVSLKDKVVIPKAEEKIFDDIA
                     LDTYYEHSKKRIVAIGTSTGGPSALIKIFERLPANLSCPVMVVQHMPPGFTKAFAERL
                     NALSPLSVKEAEDGDILRSGWAYVAPGHSHMELVQRNGVDTISLNQHDKVSGHRPSID
                     VMFRSIARVYSSDVTAVIMTGMGRDGASGLLELSQKGSYTIAQDRATSVVFGMNRAAI
                     EMGAVKNIVPLDEISKKIVEHL"
     gene            complement(1853..>3693)
                     /locus_tag="P5123_12575"
     CDS             complement(1853..>3693)
                     /locus_tag="P5123_12575"
                     /inference="COORDINATES: protein motif:HMM:NF013731.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="chemotaxis protein CheW"
                     /protein_id="HRX17143.1"
                     /translation="SEQGMGTRFIMAFPLTLAIIPAIMVSVKDETYAIPLTDVIETIN
                     ISHKDITTIEGHEVINLRGEILSLVRLSQFVGVPLAFENEDDIPVIVVGYSSRKIGLI
                     VDALEGKLEIVIKSLETNFRTVEGLAGASILGDGSIALILDVSSMINKVINEQERMSP
                     EEKMALIKKQEEHYHAMKALELDAESDVVAEKSEFSLPVQDEDGETVDHMQKDYRTSG
                     EEAYQSDIGSLTLNKKDSVDDISDYNYVKDEASNKQSADSGSSNMDDYSLLKENSEKV
                     SEDSDVDDLQSDITSDIEDYEEEIDKEIEDKGAVDGDTDEDKAVIDNETETVQKSVSS
                     VTDSDSLNEAIKAEDITSSLEAVDVFKNETLPVTGHPLGEADEKVKEALKNFKHELNS
                     NMRSALSNGDPDEHIKKKLSLNEDDLAKIKLLANVGITQAADSLSKIIDKRVDMAIPD
                     VRLVPASKILDIEKHDVQKYVSVYMPLIGDINGTVIFNLPENSAYSLVDDLYSLKTGK
                     TSALDEDGISALKEITNITGSSVINAFSERTGLSILSQVPNLLHDSLEAAISKIADEQ
                     YNVSNDYALTMDTEFYYEDDRIVGHLLILPDVKSLRVLVDNLRDNG"
BASE COUNT         1097 a          839 c          607 g         1150 t
ORIGIN      
        1 tagctataaa ctcagaagca acggcaacat tttgttcttc catctgcttc agataagaga
       61 tatactgttc agtaatttta gaaatagaaa catcaataat gtcaatttta gaccgtttga
      121 taagattcca caacaaatca agtggccctt caaaattatc tatatgaatg acatattttt
      181 cctgcgtatt ttcggattcg tcagacgtta cttgtgcaga ctgatggata tctccattat
      241 ctttttcgtt attcatagaa ctcccggcag gtcaaaaaaa tttaaccggt taaaccggtt
      301 aaaagtattt tttagaataa tagaaagact gataacaaca ttatacaaat cagctattaa
      361 gggctttttc cgcggctttc tgaagtcgtt cgggaggaaa gggtttaaca acaaaatctt
      421 ttactccaag tttaattgcc tttgttaaca gattttcctg cccaagagcc gtaaccatga
      481 taatcttggc attaggatca agtttaagaa tctcctcagc agcctctata ccgtcttttt
      541 cacgcatggt aatatccatt gttacaagct caggcttaaa ctttttatac atatctatcg
      601 caacattacc attctcggct tctccaataa tctcgtgacc tttcggagtt aaagcatctt
      661 ttacgagagt tcgcatgaat ttcgcatcat ctactactag gatcctagcc attactttct
      721 ccacgttcaa attgtaaata aaactaatac tcaaatacta atgatagtac tactacttta
      781 taataactat tacaaatgtt caacaatttt tttagatatt tcgtccaagg gtacaatatt
      841 tttcacagcc cccatctcga tcgcggcacg attcatgccg aaaacaacag aggtcgctct
      901 gtcctgcgca atcgtatagc ttcccttttg agacagttcc aaaagccccg aagcaccatc
      961 tctgcccata ccagtcatta tgacagctgt aacatcggaa gagtaaactc tggcaattga
     1021 tctaaacata acatcaattg aaggacggtg accggaaacc ttatcatgct gattaagaga
     1081 aatcgtgtca actccgtttc tttgaacaag ttccatgtgt gaatgaccag gagccacata
     1141 tgcccatcca ctacgcagta tgtctccatc ttcagcttct ttaaccgata aaggagaaag
     1201 cgcgttaagt cgttccgcaa aagccttggt aaacccaggc ggcatatgtt gaacaaccat
     1261 tacaggacag ctcaaatttg ccggcagacg ttcaaatatt tttattaatg ctgatggccc
     1321 tcccgtagaa gttcctattg caacaatacg cttttttgaa tgttcataat atgtatcaag
     1381 agcaatatcg tcaaagattt tttcttcggc tttaggtatt acgaccttat ctttgagcga
     1441 aacagcggaa taatttattt ctgccttcgc atatgccttg accttttgaa caagctgttc
     1501 ggatatttcg tcaaagctca tctgcaccat agcctgtggc ttgggtacaa aatcagctgc
     1561 accatattca agagctttga acgttgcctc ggccccatac tgagtcaaag agctgagcat
     1621 gataaccggt tttgcattct tgtccattat ctcgcgaaga gcctcaaggc catccataac
     1681 gggcatctct atatccatgg taataacatc aggataaaga acaaaacttt taaagatcgc
     1741 atttttaccg ttgtttgcgg tacctactac ttcaatctcc ggatcctttt cgagtgcatc
     1801 agagattatt ctacgtacca gagcggaatc atcaaccacc agtacacgaa ttttatccat
     1861 tatccctcag gttatctaca agaactctta acgacttgac atcaggaaga attagcagat
     1921 gccccacaat gcggtcatcc tcataataga attcggtatc catagtaaga gcatagtcgt
     1981 ttgaaacatt atactgttca tcagcgattt tacttattgc tgcctcaagc gaatcatgta
     2041 ataaatttgg tacctgcgac aagatagata accctgttct ttccgaaaag gcattaatta
     2101 cggaagaacc tgtaatattt gttatctctt ttaaagccga tattccatct tcatcaagcg
     2161 cactcgtctt accagtcttt aatgaataga gatcatcaac caaagaatat gccgaatttt
     2221 caggaagatt aaatataacg gttccattaa tatcgccaat taaaggcata taaacagaaa
     2281 catatttttg gacatcatgt ttttcgatat ccagaatctt tgatgcaggc accaaacgca
     2341 catcaggtat ggccatatcc actcgtttat caataatttt tgacaaagag tccgcggcct
     2401 gtgtaatgcc aacgtttgcc agaagtttga tcttagcaag atcatcttca ttcaaagata
     2461 gttttttctt tatgtgctca tcaggatctc cgttggaaag agctgatctc atatttgaat
     2521 taagttcatg tttaaagttt ttcagagctt ccttgacctt ttcatcggcc tctcccaaag
     2581 ggtgacccgt aacgggcaat gtctcatttt taaacacatc aacagcttca agagatgaag
     2641 ttatgtcttc cgcctttatt gcttcattca gcgaatctga atctgtaaca gaagaaacag
     2701 atttctgtac cgtctcggtc tcattgtcaa ttacagcttt atcttcatca gtatcgccgt
     2761 caacagcccc tttatcttct atctctttat ctatttcttc ttcataatct tcgatatcag
     2821 atgtaatatc agattgtaaa tcatcaacat cagaatcttc gctaactttt tcagagttct
     2881 cttttaacag actatagtcg tccatattcg aactgcctga atcagcagac tgcttattcg
     2941 aagcttcatc tttaacataa ttataatctg aaatatcatc aaccgaatct tttttgttta
     3001 atgtcaaact accaatatct gactggtaag cctcttcacc cgatgtacga taatcttttt
     3061 gcatatgatc gacagtttct ccatcctcat cctgcacagg aagagaaaac tcgctttttt
     3121 cggcaacgac atcagactca gcatccagct caagagcctt catcgcatga taatgctctt
     3181 cctgtttctt aatcaacgcc atcttctctt caggagacat cctctcctgc tcatttatca
     3241 ccttgtttat cattgacgac acatcaagga tcagagcgat acttccgtct cccagaatgg
     3301 acgcgccagc aagaccttca acagttctaa aatttgtttc aagagatttg attacaattt
     3361 caagtttacc ctcaagggca tcaactataa gaccaatctt gcggctactg tatccaacta
     3421 caattaccgg tatatcatct tcattttcaa acgcaagagg aacgcccaca aactggctaa
     3481 gacgaacaag agatagtatt tctccgcgta aatttatgac ttcgtgtccc tcgatcgttg
     3541 taatatcctt atggctaata ttgattgttt caattacatc ggttaaaggt atggcgtaag
     3601 tctcgtcctt tacagaaacc ataatagccg gaataattgc cagagtaagc ggaaatgcca
     3661 taataaaacg ggtacccatg ccctgttcac tct
//
LOCUS       DAOTGZ010000262         3649 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_3064
            NODE_30078_length_3649_cov_4.854758, whole genome shotgun sequence.
ACCESSION   DAOTGZ010000262 DAOTGZ010000000
VERSION     DAOTGZ010000262.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868088
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 3649)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3649)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:22:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,785
            CDSs (total)                      :: 2,751
            Genes (coding)                    :: 2,732
            CDSs (with protein)               :: 2,732
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 12 of 19
            Pseudo Genes (internal stop)      :: 5 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3649
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_30078_length_3649_cov_4.854758"
                     /isolate="AMR_MDS_3064"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..269)
                     /locus_tag="P5123_12580"
     CDS             complement(<1..269)
                     /locus_tag="P5123_12580"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRX17144.1"
                     /translation="MEKIKLSLKIPDFLSQFKNRNNYEDFFLKERKVLLTNLGIYADL
                     KKDIVKKAPETVYKLVYKPKNAKLFKDAAGNIKGVFYRDGKILKHA"
     gene            complement(292..969)
                     /locus_tag="P5123_12585"
     CDS             complement(292..969)
                     /locus_tag="P5123_12585"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRX17145.1"
                     /translation="MDKTIEFLDAIGTYDKMFVYRTFSVPSTENVQSMSSRAVGEKTS
                     KKNSTKSTFVGMMILVALIVVFVFGLNSYLTYKHDKEVQEKTQYNANMFLLSIINAIS
                     NLEKGMYEIKGGFDEYQDRNNSHTDLDTVMKPYLSKAEWYWDMAETDLSEAFDSMSAL
                     KKLIDDKTYDHFVDMYASTREYKEAVSPYGKNSMQYNNDVSGANTMIHNAIARFKSYI
                     YPITNEE"
     gene            1377..2993
                     /locus_tag="P5123_12590"
     CDS             1377..2993
                     /locus_tag="P5123_12590"
                     /inference="COORDINATES: protein motif:HMM:NF013752.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HRX17146.1"
                     /translation="MKEMYQPNNSHRRKRLFGIENTLPKKLQKKLKTTMYPLFYESVF
                     CQIDESKFAPLYSEKASRPNCPVNIFVGLEIFKHLFVLSDEQLMENFYFDVRYQVAFG
                     IQEMDEALYSLKSLYNFRRRILLYEEKTGINLIKEVFDELVMGFIEATGTSTQIIRMD
                     STQLHSKMKALSRIDIMSRTLPRFIRELSQEEQSKYKEYAPYLNKEELSTCLAKIKHE
                     DESLKTIATEIVIILKRYESSDYAQTKAYGLLERVLTDQTIIIDEQLEIKDNKDIAPD
                     SCQSPVDEDATYRKKGGKGYHGYCANISETADKDNETQLITSVSVEPNIYSDIAFLKD
                     ELEDLKAKTDMDILIVDGGYTNEATRELAKESNTTLIETGIKGKRPEKTTEDFDIDEE
                     KGIMRCPAGKKPMQTRIRSGKAQGLFSHEDCQDCPYRENCFAKKQMKGMKVDIALSRY
                     ARDKQRAYSETKEFYDLKRLRAGVEGAISTLKRKGLNAIQVVGKTRVRMTVTYAAIAS
                     NFKRLFKVTTKRRKAIQKAFSPQSVWVNAV"
     gene            3177..>3649
                     /locus_tag="P5123_12595"
     CDS             3177..>3649
                     /locus_tag="P5123_12595"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF016464.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006486 - protein glycosylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 61 protein"
                     /protein_id="HRX17147.1"
                     /translation="MNKKNLKIIPLKKSVFYKIEELVYPSNSSFMPINVKDFSSYASD
                     YIVSSSALNFVRTRVLGHYGIETKQAVGTKKIIISRKNLKHARLKNERRVLELMSNRG
                     FEIIFPECMSLQEQVRVFSQAGVVAGTTGAAFTNLIYCPKNALIMNIIPKRYNFFL"
BASE COUNT         1231 a          693 c          665 g         1060 t
ORIGIN      
        1 gcatgcttta atatttttcc atccctatag aaaaccccct tgatattccc tgctgcatct
       61 ttgaacaatt tagcattttt aggtttgtac accagtttat atactgtttc tggtgctttc
      121 tttactatat ctttctttaa atcagcatat atccctaaat ttgtaagaag aacctttctt
      181 tcttttagaa aaaaatcttc gtaattattt ctgtttttaa actgtgatag aaaatccgga
      241 atttttaatg atagttttat cttttccata tttcctcctt cctatacgag tctactcttc
      301 attcgtaatt ggataaatgt agcttttaaa tctggcaatg gcattatgaa tcattgtgtt
      361 tgcaccacta acatcattgt tatactgcat ggaatttttc ccgtatggag aaaccgcttc
      421 cttatattct cgagtacttg cgtacatatc cacaaaatga tcgtaggttt tatcatctat
      481 cagttttttc aaggcactca tagagtcaaa cgcctccgaa agatctgttt ccgccatatc
      541 ccaataccat tcagctttgc tcaaatacgg cttcatcacg gtatctaaat ccgtatgaga
      601 attatttctg tcctgatact catcgaaacc tccttttatt tcatacattc ctttctccaa
      661 gtttgaaatg gcattaatta tcgagagcaa aaacatattg gcattatact gagtcttttc
      721 ttgtacttcc ttgtcatgct tgtaggtgag ataactattg agcccaaaca caaaaacaac
      781 tatcaatgct accaaaatca tcattccgac aaacgtcgat tttgtactat tcttctttga
      841 tgttttttca cccactgctc tggatgacat agattgcaca ttttctgtac ttggaaccga
      901 gaatgttcta tacacaaaca ttttatcgta tgtcccaata gcatccagaa actcgatcgt
      961 tttatccaaa gaatatccct tttctctgca gtgaatgatt aactgtacaa gttcgttctt
     1021 ttttatcttg cttatttttt tctcctccgt attatttctt tagtatttta ttttattaat
     1081 tattatatat agtctatatt tataattata catgtataaa atcaaccttt aacttaatat
     1141 ataattaaat aattatattt ataaatgact tttatcgaaa attttgtcag aaaatcacca
     1201 aatattttta gatttctccg cacataatct tttaaaaagg agtacaacat ctaattatta
     1261 aaatttaatg attaccctgc aaaaagcaaa ggcgaacaca aacaaaagaa gcagaagaaa
     1321 aacaatgttt tttaacataa acatggtata atagaggtga taaagcggga caggagatga
     1381 aagagatgta tcaaccgaac aacagccacc gacgaaaacg actcttcgga atagagaaca
     1441 cattaccgaa aaagctgcaa aaaaaactga agacgaccat gtacccactc ttttatgaaa
     1501 gcgtcttctg tcaaatagat gagtcaaaat ttgcaccctt gtacagtgag aaagcgtcga
     1561 gacctaattg tccagtgaac atctttgttg ggctggaaat ctttaaacac ttattcgttc
     1621 tgtccgatga gcaactgatg gaaaacttct actttgatgt cagataccag gtggcatttg
     1681 gtatacagga gatggatgag gcgttgtatt ccctcaaaag cctgtacaat ttcagaagaa
     1741 gaattctgtt gtatgaggaa aaaacaggga ttaacctaat caaagaggta ttcgacgaac
     1801 tggtcatggg attcatagag gcaacgggga caagcacaca gattatcaga atggactcga
     1861 cgcaattaca cagcaaaatg aaggcacttt cacgaataga tatcatgagc cgaacactgc
     1921 cgcgattcat ccgagaactg agtcaggagg aacaaagcaa atacaaggaa tacgcgccgt
     1981 atcttaataa agaggaatta agcacctgcc ttgcgaaaat aaaacacgaa gacgaaagtc
     2041 tcaagacaat cgccacagaa atcgtgatca tcctcaaacg atatgaaagc agtgactatg
     2101 cccaaacgaa agcatacggt ttacttgaac gcgtattgac agatcagacg atcatcatag
     2161 acgaacagtt ggaaataaaa gacaacaagg atatcgcccc tgattcctgc cagagtccgg
     2221 tggatgaaga cgcaacttac cgaaaaaagg ggggaaaagg ctatcacgga tactgtgcaa
     2281 acatctcaga gacagctgac aaagacaacg agacccaatt gattacaagt gtaagcgttg
     2341 aaccaaacat atactctgac attgcctttc tcaaagacga attagaagac ctcaaagcta
     2401 agacagacat ggacatcctc attgtagatg gcggctatac caacgaagca acacgggagc
     2461 tggcaaaaga atcgaacacg acccttatag aaacgggaat aaaggggaag agaccggaga
     2521 aaacaaccga agattttgac atagatgaag aaaaaggaat catgagatgt ccagcgggaa
     2581 agaaacccat gcagacacga atcagaagcg ggaaagctca gggattattt tcacatgaag
     2641 actgtcagga ttgtccatac cgagaaaatt gttttgcaaa gaaacaaatg aaaggcatga
     2701 aagtagatat tgccctctcc cggtatgcaa gagacaaaca gcgcgcttac agcgagacca
     2761 aagaattcta cgaccttaag cgcttgagag cgggtgttga gggagcgatc agtactttga
     2821 aacgaaaagg attaaacgcc atacaagtgg tgggtaagac acgtgtgcgg atgaccgtga
     2881 cttacgctgc gattgccagt aacttcaaga gactgtttaa agtaacaacc aaaaggcgaa
     2941 aggcaattca aaaggctttt tctccacaaa gcgtgtgggt gaatgccgtc taattgaaga
     3001 aaaaagaggt gattatgcca tctgtaagag aaaattgacc ctgttttgag cattattctt
     3061 caaaatgata tcgtttttca gactattttt tcttgtgtct aaatggtctg tgaaatttgt
     3121 tctttttggc tttttctcct ttttgaaaat aagagttttt gcagggtaat catataatga
     3181 ataagaaaaa tctcaaaatc ataccgctca aaaaatcagt gttttataaa atagaagaac
     3241 ttgtatatcc atccaacagt agttttatgc caattaatgt aaaagatttt tcttcctatg
     3301 cttctgatta cattgtttcc agtagtgctt taaattttgt cagaacaaga gttctaggcc
     3361 attacggaat agaaacgaaa caggctgtag gtaccaaaaa aataataatt tcacggaaga
     3421 acctcaaaca tgctcgctta aaaaatgaaa ggagagtttt agagttaatg agtaatcggg
     3481 gatttgaaat tatttttcct gagtgcatgt ctcttcaaga gcaggtaagg gttttttcac
     3541 aagcaggtgt tgtcgccgga acaaccggag ctgcctttac aaatctcatt tattgtccaa
     3601 aaaacgccct gataatgaat attatcccca aaagatacaa ttttttcct
//
LOCUS       DAOTGZ010000263         3612 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_3064
            NODE_30465_length_3612_cov_4.318808, whole genome shotgun sequence.
ACCESSION   DAOTGZ010000263 DAOTGZ010000000
VERSION     DAOTGZ010000263.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868088
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 3612)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3612)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:22:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,785
            CDSs (total)                      :: 2,751
            Genes (coding)                    :: 2,732
            CDSs (with protein)               :: 2,732
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 12 of 19
            Pseudo Genes (internal stop)      :: 5 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3612
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_30465_length_3612_cov_4.318808"
                     /isolate="AMR_MDS_3064"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..183)
                     /locus_tag="P5123_12600"
     CDS             complement(<1..183)
                     /locus_tag="P5123_12600"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRX17148.1"
                     /translation="MNNERLIIERTKNTKGTLLCASFKKTEESTRVQIRHSLCLIHYY
                     VSGSIFFLSSVIKISGS"
     gene            complement(219..1412)
                     /locus_tag="P5123_12605"
     CDS             complement(219..1412)
                     /locus_tag="P5123_12605"
                     /inference="COORDINATES: protein motif:HMM:NF025019.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HRX17149.1"
                     /translation="MKIKRNILNQLLSEQDRAEVSILLGPRQVGKTYLLLELCNIYKN
                     NGLRTKYYNLEIPSHSIEFNKNDNELFEMLTTDVDIVFIDEFHYLKNASKLFKAIYDS
                     DKKIKIYASGSSSIEIHKHLQESLAGRRLVTKIFPLSLDEYKQKKALSHVDNYLVYGG
                     LPELVHFNNKDEQMRILREILETYIQKDIKSLIKEENIRAFNNLLYLIAENQGSIVQL
                     SNLASEVGLSSKTISHHLSILENTYVIYPLHSYSKNLGNELKKSHKYFLYDSGIRNAM
                     LKDFGNPSHREDKGALYESLIMTELFKQLKPNMELKFWRTKQGDEVDFVILINRKPYL
                     IEVKTSLSGIEIPAGIRKFINKYPETIGATVFCLQRKPSIIYQNIEIRFLRHEDVINF
                     LSNYV"
     gene            complement(1521..>3612)
                     /locus_tag="P5123_12610"
     CDS             complement(1521..>3612)
                     /locus_tag="P5123_12610"
                     /EC_number="1.17.4.1"
                     /inference="COORDINATES: protein motif:HMM:TIGR02504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004748 - ribonucleoside-diphosphate
                     reductase activity, thioredoxin disulfide as acceptor
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="adenosylcobalamin-dependent
                     ribonucleoside-diphosphate reductase"
                     /protein_id="HRX17150.1"
                     /translation="LFYEALTSGKFIPAGRILANARENSPMKNYNNCFTIDINDSMTD
                     IYESLKEDALISKMGGGVGFDISKLRPKGQTLSGGGESSGPVSFLRIFDQSAKTIMTG
                     GQRRSAHIALMDISHPDIEEFITAKQGDQNKELTQFNISVKIPDAFIDALNNDEDWNL
                     VFDGKIYKTVKARDLYTLLAKNAFTHNEPGIFYSDKVEQFNNGYWAFKLDRVNPCGEL
                     VMPAYSLCCLGALNLTRFVIDPFSDKARFDHDGFSRCAATGVRFLDNVLERTSYPLEK
                     IETNAKKWRRIGLGFTGLGDLFAMMGIVYGSSTSVKLAGEIAQKLRDASYIASCELAK
                     ERGTFPGFNKEKYLKANFIKELPKHIRDMISTNGIRNIQLNTVAPTGTISLSVGQNCS
                     SGIEPIFSLEYTRHIRNGSGTETKPEKVLDYAWLLYKKIFGDSTVPQYFVTAPSVDPY
                     ASIDIQSAVQKYIDHSISKTLNLPRGTKFDDYQKLFLYAHKKGLKGFTTFNPDGSMKG
                     ILESDDRANKEKVHRRYAPNRPKELPCIVHKLKQASKTYIIITGFLNNSLYEIFCLDD
                     IDNVIQIADGSKAIVRKNGKASYDLIQISSKNRLAYSNFTRKFASPDSSLARFISMAL
                     RHGTPLQFVINQLSRDTSFADFERVLVRILKKYINNGEEVISGVNECPECNDNLVFRD
                     GCVVCPSCGWSSCL"
BASE COUNT          983 a          670 c          751 g         1208 t
ORIGIN      
        1 tgatccggat atttttatta cggagcttaa aaagaaaatg gaaccggaaa cataataatg
       61 tatcagacac agggagtgtc tgatttgcac tctcgtactc tcttctgttt ttttgaaaga
      121 ggcacacagc agtgtgccct tcgtgttctt tgtcctttcg attattaatc tttcattatt
      181 cattgtcgtc caactgtctt accatttctt ggtgataatt aaacgtaatt acttaaaaaa
      241 ttaataacat cttcgtgtct taaaaatctg atttctatat tttggtatat tatgctgggt
      301 ttcctttgta agcaaaatac tgtagcgcct attgtttcag gatacttatt gataaatttc
      361 ctgattccag caggtatttc gatgccgcta agggaagttt ttacctcaat caggtatggt
      421 ttgcgattaa taagaattac aaaatctacc tcgtcaccct gttttgttcg ccagaatttt
      481 agttccatat tgggtttcaa ttgtttaaac agctctgtca ttattaaaga ttcatataag
      541 gcacctttat cttctctatg gctgggattt ccaaagtctt ttaacatggc gtttcttatg
      601 ccgctatcgt aaagaaaata tttgtgtgat tttttaagtt catttcccag atttttggaa
      661 tacgaatgca aggggtatat cacatatgta ttttctaaaa tggataggtg atgactgatt
      721 gtcttggaag atagtccgac ttctgaagcc aggttgctta gttgaacaat agatccctga
      781 ttttcggcta taagataaag aaggttatta aaggctctta tgttttcttc ttttattagg
      841 gatttaatat ctttttgaat gtatgtttca agaatttctc gcagtatacg catctgctca
      901 tccttgttgt taaaatgaac cagctccgga agaccaccat acacaagata attgtcgaca
      961 tgagatagag cttttttttg cttatattca tcaagtgata gaggaaagat tttggtaacc
     1021 agccttctgc ccgcaaggct ctcttgtagg tgtttgtgaa tttcaattga tgaagaaccg
     1081 gaggcgtaaa tcttgatttt tttatcgcta tcgtatattg ccttaaaaag ctttgatgca
     1141 tttttgagat aatgaaattc atcgataaaa acaatatcca catcagtggt gagcatttca
     1201 aagagttcat tatcattttt gttaaactcg atgctgtgcg aagggatttc cagattataa
     1261 tattttgtgc gtaatccatt atttttgtag atgttgcata gctcgagtaa aagatatgtt
     1321 ttgccaactt gtctcggccc cagaagtatt gagacctccg cacggtcttg ttcggaaagt
     1381 aattggttta agatatttcg tttaatcttc atttgactat tctggagtag ttctacagaa
     1441 tagttaaatg atatttgtaa taaataagct tggatgggtc tgcaatataa tcattttaac
     1501 aactaataaa agcttataat ttacaagcag ctgctccatc cacaggatgg gcataccaca
     1561 cagccgtcac gaaatacgag attatcgttg cattcgggac attcgtttac accgcttatt
     1621 acctcttcgc cgttgttgat atattttttg agtatccgca caagaactcg ctcgaaatcg
     1681 gcaaaactgg tatcgcgcga aagttgatta atcacaaatt gaagcggggt tccatgtcta
     1741 agagccatgg atataaaacg agccaatgat gaatctggtg atgcaaattt gcgagtaaag
     1801 ttgctgtagg caagcctgtt cttgctgctt atctggataa ggtcgtaact tgctttgccg
     1861 tttttgcgca caatcgcttt tgagccgtcc gctatttgta taacgttatc aatatcatca
     1921 aggcaaaaaa tctcgtacag cgagttgttt aaaaaacctg tgataataat ataggtcttt
     1981 gaggcttgct tgagtttgtg cacaatacag ggaagttctt tggggcgatt gggggcatag
     2041 cgtcggtgaa ccttctcctt gttggctcgg tcatcgcttt caagtattcc tttcatgctt
     2101 ccgtcgggat taaaagttgt aaacccttta agaccttttt tgtgcgcata caaaaatagt
     2161 ttttgatagt catcaaattt tgttccgcgc ggaaggttaa gtgtttttga gatgctgtga
     2221 tcgatgtatt tttgtacggc cgactgaata tcaattgatg catagggatc aacagatggc
     2281 gctgttacaa agtattgtgg aacggttgag tcaccgaaga tttttttata aagcaaccag
     2341 gcatagtcca aaaccttttc cggtttagtt tcggtaccgc tgccatttcg gatatgtctt
     2401 gtatattcca gtgaaaagat tggctcgatt ccagaagagc agttttgacc aaccgaaagc
     2461 gaaatggtgc ccgttggtgc gacagtattc agttgaatat tgcgtattcc atttgtacta
     2521 atcatatcac gaatatgttt tggtaattct ttgataaagt tagcttttag atacttctct
     2581 ttattaaatc ctggaaaagt tcctcgctct ttggctagtt cgcacgaggc gatgtaagat
     2641 gcgtcgcgaa gtttttgtgc gatttctccg gcaagtttaa cagatgttga tgagccataa
     2701 acgatgccca tcattgcaaa aagatcgccc aaaccggtaa aaccgagacc gatgcgtctc
     2761 catttttttg cgttggtttc aattttttca agagggtagg atgtacgttc cagtacattg
     2821 tcaagaaaac gaacgccagt tgccgcgcat cgtgaaaatc catcatggtc aaatcgtgct
     2881 ttgtcggaaa aagggtcgat aacaaatcgg gtaaggttaa gagcgccaag acagcagagt
     2941 gaataagccg gcataacaag ctcgccacag gggtttaccc tgtccagttt aaacgcccag
     3001 tatccgttgt tgaactgttc taccttgtca ctgtaaaaaa tacccggctc gttatgcgta
     3061 aaggcgtttt tagcaagaag agtgtaaaga tcacgggctt ttactgtttt gtatattttt
     3121 ccatcaaata ctagattcca gtcctcgtcg ttatttaggg cgtcgataaa agcatcagga
     3181 atttttaccg agatattaaa ttgtgtcagc tccttgtttt ggtcaccctg tttggcggta
     3241 ataaactctt cgatatccgg atggctgata tccataagcg caatatgtgc cgagcggcgt
     3301 tgtcctcccg tcattattgt ttttgctgat tgatcaaaga ttcgtaaaaa tgagacaggt
     3361 cctgacgact cgccaccacc agaaagagtc tgccccttag ggcgaagttt cgagatatca
     3421 aaacctacgc caccgcccat tttgcttatt agggcatctt cttttaacga ttcatagatg
     3481 tcggtcatgg aatcatttat atctatggta aagcagttgt tatagttttt catggggctg
     3541 ttttctcgcg cgtttgcaag aatgcggcca gctggtataa attttccact tgtcagggct
     3601 tcgtaaaata gc
//
LOCUS       DAOTGZ010000264         3608 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_3064
            NODE_30500_length_3608_cov_4.874191, whole genome shotgun sequence.
ACCESSION   DAOTGZ010000264 DAOTGZ010000000
VERSION     DAOTGZ010000264.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868088
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 3608)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3608)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:22:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,785
            CDSs (total)                      :: 2,751
            Genes (coding)                    :: 2,732
            CDSs (with protein)               :: 2,732
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 12 of 19
            Pseudo Genes (internal stop)      :: 5 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3608
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_30500_length_3608_cov_4.874191"
                     /isolate="AMR_MDS_3064"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1613)
                     /locus_tag="P5123_12615"
     CDS             complement(<1..1613)
                     /locus_tag="P5123_12615"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRX17151.1"
                     /translation="MDRKGINFILLSGGLIAAVFICYLLRWYIFDLPFSSTQQFNYPS
                     YAITDNDSNIYVIDTSLRRVVKLDGQGRLLFVIEGGRREEGTFFYAADLAVDNEGNLY
                     VLNWLHDEGGFHVEKEEILQYSSDGQFRQIVYSRPYKDRTSTRVQRGQLNALSFIDDK
                     ISWFDIASDSVTRFIFDNKTGSVKETAVLSYPEANIFVADVAVATDTKWAVVNKKGQI
                     TLHDLTGNSSAGLYNADDNLSEDISVPLMVEADAGGGLYYSDIGTGTIQGLGDKQGYI
                     FSDESLKKAGYDVQDMVYYRFDINGDVLTTINDYGVVQTGIDGTVINYYDSIRIPFHE
                     LALKLLSYFMLAAVLMLLVLVLRHVYLYMFQRTVPPVLINALSILFVIGVTTLLVVTV
                     VLKNFAARYTNSLLEKTQQMAQLIPQTVDGDLFHQIQSRDDYMGDAYREVRASLHEAL
                     NENMDPWNDRYYFVLYSVINEKLYGMMYLNDRISMYHPFNYYEETEGAYRLAHNGEIS
                     AETDTDNWGTWIYAVSPIRDSKGKVVALL"
     gene            2046..3251
                     /locus_tag="P5123_12620"
     CDS             2046..3251
                     /locus_tag="P5123_12620"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRX17152.1"
                     /translation="MSDANLRAMGTAVFSEAQSKGKRLLAWYARPFVGDRSLLTLHEL
                     TGIAADSGQSFSGEEDIRLNLIVGSENRKNDFAEGFLPIRVDMGERWINVWMRMSQGL
                     LEEPVNVFKYGNVYFIRDGHHRVSTALRLGRKYIRARVTRLTTPYCLNCCFSRKDLPF
                     FRKLVNFNKKTRFLDNVHHAKFDIRRNNSWNILENEIRKWSPSWVHRHKNHSWVKEDF
                     KRFLPDNENLPENIEQQDVMDRFWYYSTYKYIMDFVKRRSLHYLFPRWGDTDVAMELI
                     NIWNRYENPDQFTLEDIYTIFINHYRKKHFFMVPFQIIAEQIHQIRRTASDERNRFMI
                     NSNIQTFRPEFNPPANSGKKEWRIMYNDLFVTHATLMKNRLKRPPFLAELVSDWYDNV
                     YSVKFKTES"
BASE COUNT         1103 a          730 c          722 g         1053 t
ORIGIN      
        1 tccagcaggg ctacaacttt acctttagaa tcacggattg gtgaaacggc atagatccag
       61 gtgccccagt tgtctgtatc agtttcggca gatatttcac cgttatgcgc cagacgataa
      121 gcgccttctg tttcttcgta atagttaaac ggatgataca ttgagatacg gtcgttcaga
      181 tacatcatcc cgtaaagttt ttcattaata acactgtaca gtacaaaata gtatcgatcg
      241 ttccacggat ccatattttc gttaagcgct tcatgtaatg aagcacgaac ctcccggtaa
      301 gcgtcaccca tatagtcatc ccgggactgt atctgatgaa aaagatcgcc gtcgacagtt
      361 tgaggtatca gctgtgccat ctgctgggtt ttttcaagaa gagagttggt gtaccgggca
      421 gcaaagttct ttaatacaac ggtgacaaca agcaaggttg ttacaccaat cacaaacagt
      481 atggacaagg cattaataag aaccggcggc acagttcgct gaaacatgta cagatagaca
      541 tgccgtaaaa ccagaaccag aagcataaga acggcagcca gcataaaata agataataat
      601 tttaatgcaa gttcatgaaa aggaatcctt atgctgtcgt agtagtttat aaccgtaccg
      661 tcaataccgg tttgaacaac accataatca ttaattgtgg ttaaaacatc accattaata
      721 tcaaaccggt aatagaccat atcctgaaca tcgtaaccgg cttttttaag agactcatcc
      781 gaaaaaatat atccctgttt gtcgcccagc ccctgaattg tccctgttcc aatatcggaa
      841 taatataggc ccccgcccgc atcggcttcg accattaaag gaacagaaat atcttctgat
      901 aaattatcat ccgcgttgta aagaccggct gacgaattgc cggtcagatc gtgcaacgtg
      961 atttgtccct ttttgttgac aactgcccat tttgtatcgg ttgccacagc tacatcggca
     1021 acaaatatat tggcctcagg gtaagataga accgcagttt ctttaacaga acccgttttg
     1081 ttgtcaaaaa taaaacgcgt cacagaatca gaggcaatat cgaaccagct tattttgtca
     1141 tcaataaacg ataaagcgtt taactgcccg cgttgtaccc ttgtagaagt tctatcctta
     1201 tacggtcttg aatagacaat ctgcctgaat tgaccgtcag atgaatattg aagtatctcc
     1261 tctttttcaa catgaaaacc gccctcgtcg tgaagccagt tcagtacata aaggttgccc
     1321 tcgttatcaa ccgcaagatc cgcggcgtaa aagaatgtcc cttcttcacg gcggcctcct
     1381 tctataacga aaagaagcct gccctggccg tccagcttga ctacacgccg caaagatgta
     1441 tcaattacgt aaatattgct gtcgttatct gtgattgcgt atgatggata gttaaactgc
     1501 tgggttgacg agaacggaag atcaaaaatg taccatcgca gcagataaca gataaaaaca
     1561 gctgcgatta aacctccgga aagtagaata aaattaatac cttttctgtc catgattgcc
     1621 taccggagta tagaggatta ttacttaatt ataattcttt tcagaatgga tgtcaaatag
     1681 ttatagttat cttctgataa ttatttatta actggaactt gaggaaaatt gtctttaata
     1741 tgctcaaagc gtaaattctt tccggaaatg gcaattttcc gaagttcact atacattttt
     1801 ttataacaag cagtgtaaag tctgttttaa tgcctgcagc tgtattcttc agctattgtg
     1861 tttccacttt tcatgagccc tgctgacggt ttaaactatt agcgcaacaa ccgacaaaaa
     1921 gttgacattt gacaactttc aggcttatta tggtataaaa gcatacatga tttattaact
     1981 gtttcttctg ttaatgtaaa aaaccaatat catgcgaata gagatactgc tgccggggaa
     2041 taattatgag cgatgccaac ctgcgtgcca tgggtactgc cgtttttagt gaagctcagt
     2101 caaagggcaa aagactatta gcctggtacg cacgcccctt tgtcggtgat cgttctctgc
     2161 tgaccctgca tgaactgacc ggaattgccg cagactccgg acaatccttt tcgggtgaag
     2221 aagatattag gcttaaccta attgtgggca gcgaaaaccg caaaaacgat tttgccgaag
     2281 gttttcttcc tattcgagtt gatatgggag aaagatggat aaacgtctgg atgcgcatga
     2341 gccaggggct gctcgaagaa ccggtcaatg tttttaaata cggcaatgtc tactttatac
     2401 gcgatggcca ccatcgggtt tcgacagcac ttcgtcttgg aagaaaatat atacgggcac
     2461 gggtcacaag actcacaacc ccatactgtc ttaactgctg cttcagccgc aaagaccttc
     2521 cctttttcag aaaacttgtg aattttaaca agaagacaag gtttctggat aatgttcacc
     2581 acgctaaatt tgatattaga cgcaacaata gctggaacat tcttgaaaat gagataagaa
     2641 aatggagccc gtcgtgggtt catcggcata aaaaccattc atgggttaaa gaagatttta
     2701 aaagattttt gcctgataat gaaaacttgc cagaaaatat tgagcagcag gatgtgatgg
     2761 atcgattttg gtactactcg acatacaaat acataatgga ttttgttaaa aggcgatccc
     2821 tgcactatct ttttccccgc tggggtgaca ccgatgtggc aatggaactg attaatatat
     2881 ggaacagata tgaaaatcct gatcagttta ccttggaaga tatttacacc atttttatta
     2941 atcactatcg caagaagcat tttttcatgg ttccttttca gataattgcc gagcagattc
     3001 accaaataag aagaaccgcg tctgatgaac ggaaccgctt tatgataaac agtaatattc
     3061 agacctttcg ccccgaattt aatccgcctg ccaacagcgg caaaaaggag tggcgaataa
     3121 tgtataacga cctctttgta actcacgcga ccctaatgaa aaaccgcctt aaacgccctc
     3181 cctttcttgc cgaactggtt agcgactggt atgataatgt ttatagtgta aaatttaaaa
     3241 cggaaagctg atctaaatat ttgcgcaacc aataaagcta caaaaacagt gcatttttta
     3301 caacatagag accgagtgtc tatgttctgt taggcattca tggtatattg ttatgttgtt
     3361 aagttagttc taatgtttta gtctgacggg actttttttt attgtaatta attttgttgt
     3421 gagtgattat aggtttaagt tttgatatgt ggagactgtg taatattatg tttaaggtac
     3481 aattattttt ttattttatt tttttaggaa agtcctcagt aaagagtact gtagtagaga
     3541 gaaaaactgc gattagtacg accatgtatt tcatagaaac ctccgtaaag agacatactg
     3601 tattttat
//
LOCUS       DAOTGZ010000265         3605 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_3064
            NODE_30534_length_3605_cov_4.555211, whole genome shotgun sequence.
ACCESSION   DAOTGZ010000265 DAOTGZ010000000
VERSION     DAOTGZ010000265.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868088
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 3605)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3605)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:22:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,785
            CDSs (total)                      :: 2,751
            Genes (coding)                    :: 2,732
            CDSs (with protein)               :: 2,732
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 12 of 19
            Pseudo Genes (internal stop)      :: 5 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3605
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_30534_length_3605_cov_4.555211"
                     /isolate="AMR_MDS_3064"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..216
                     /locus_tag="P5123_12625"
     CDS             <1..216
                     /locus_tag="P5123_12625"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRX17153.1"
                     /translation="AMMASCHNRYDAGIVPASVYVIGNSGYMDFVLNGFGQDAQLSVF
                     SIFHYLLFIALLRREAIISCQKHDCAY"
     gene            375..932
                     /locus_tag="P5123_12630"
     CDS             375..932
                     /locus_tag="P5123_12630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015387978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HRX17154.1"
                     /translation="MSRIESFNRIHILGASGSGTTSLAKALCDVLPHHHFDSDDYLWK
                     IKFTETHQKEKRLELLRDGLKNHDNWILSGAIIDWGNPLMPLFDLVVFLSVPDNIRME
                     RLCDREKKLYGDRILPGNDKYECYTEFIDWASRYEAGGTGVRSRKQQELWMANLDCPI
                     LRIEGDPTVEESVARVLDYMQKSDK"
     gene            1240..1779
                     /locus_tag="P5123_12635"
     CDS             1240..1779
                     /locus_tag="P5123_12635"
                     /inference="COORDINATES: protein motif:HMM:NF028509.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cache domain-containing protein"
                     /protein_id="HRX17155.1"
                     /translation="MNLLKFIKYVLKARAIICLILMFIIVTAIFYQRGGLASDSMDSR
                     ITKIVYQAHAFVLENNCDLSIAQKAFETDPRFIDNQKMIYIFMHSYDTVRKEVICIAQ
                     GARPELVGKNMWSLRTPEGRLIFQESITLLKGKEEFWLEYDWLNPYTNLVKTKRAFYK
                     KIVLSDGRKAWVGSGYWKN"
     gene            1802..>3605
                     /locus_tag="P5123_12640"
     CDS             1802..>3605
                     /locus_tag="P5123_12640"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase dimerization/phosphoacceptor
                     domain -containing protein"
                     /protein_id="HRX17156.1"
                     /translation="MLIKTKLWLGGAIILVILIFLASLNIVMHKNILVSLDARDQVRG
                     TLHNAEEYKKWRQDVDAFVTKMVSQNAVPAMPRNFLLLPYDQQTAETDEFIESVKNLI
                     KLVDQYESAVKSNERLFEEHRSNIISLYKKLDHKIATVLAQLQMREVLGEDVSNESSL
                     APYVLKSLNQLTLIALNSLVTRQYPDENKKTVKFNHRFLDMQLKIIDSDGSIAALFKK
                     LFIQIDSIQKLILASSANELKLSAQIGEAKKNYDRAYLLSSQEYRLHRAQSRVEKADH
                     NLQLISSRTLLLTSVFLILVPLLVIGLALLGVNRLIFLPVKNLVKIIRKFEKGDLLAI
                     AKIRSDDEIGMLSSAFNKMAEQIRQKVSDLDLLNRELGESEEKYRLLAINSEDVIFTL
                     NKDLQFTYISPSINRLRGSKESDYIGKKFSDKVPAEALDEVMKITKQLQKRIKTGSNN
                     NSGIKRFLLKLKGKNGKSFWVEITMSLMYGKGKKVFGLLGVARDITDRVRAEEKLKTS
                     LGEKEILLREVLHRTKNNMGVISSFLELQSASYKSEEVKHLVNDSTARIKTMALAHEM
                     LYNGKDISKINMRDYITGLMELLEKSNRFESDRVS"
BASE COUNT         1140 a          598 c          782 g         1085 t
ORIGIN      
        1 gctatgatgg cctcctgcca taatcgctac gacgccggca tcgtgccggc ttcagtttac
       61 gtaatcggaa attctggata tatggatttc gttctaaacg gttttgggca agacgctcag
      121 ttgagcgtct tttcgatttt tcactattta ttattcattg ccttattgcg acgcgaagct
      181 ataatatcct gccaaaaaca tgattgtgcc tattaactga cgaaatggta cacattgcca
      241 aaatacctga tttattaaac tgatttgatc tcagttatac cgttttgtac ttgaaatggt
      301 acatgtttat ggtaaactag cacttttgag tctatgattc tgattaaacc aagctggatc
      361 atctggagta aactatgagc cgtattgagt cctttaacag gatacacatt cttggtgctt
      421 cgggttcggg tacaacttcc cttgcgaagg ctctctgcga tgtattgccc caccatcatt
      481 tcgattcaga cgattatttg tggaagataa agttcacgga aactcaccag aaagagaaac
      541 gtcttgagct tttacgggat gggctcaaaa accatgataa ttggatctta tccggtgcaa
      601 taatcgattg gggcaatccg cttatgcctc tctttgacct tgtggttttt ctgtcagtgc
      661 cagataatat tcgcatggag aggctgtgtg atcgagaaaa aaagctctat ggtgatagga
      721 ttttaccagg aaacgataaa tatgagtgct atacggaatt cattgactgg gcttcccgtt
      781 acgaagctgg aggaacaggt gttcgaagcc gaaaacagca ggagctttgg atggcgaatc
      841 ttgattgtcc gattcttcgc attgaggggg atccgactgt tgaagaaagt gtagcaagag
      901 tgcttgatta tatgcaaaag tctgataaat aaccaatcga tatattggaa tttggaaaga
      961 agtattctgg gtaattttaa aagtttggca tatgccacat gataattaat gactgaaaaa
     1021 tcttttttgt tgatattctg tcgaatattg ttatcataaa cgtatattgg tttgaaataa
     1081 ttatcaatct atctggaaca taaaaaaatt actattttct tagcgcgagt catttatcaa
     1141 attcagttga gatgccggat gggtgtaatg caagcatatt gataattgaa tctaagaaag
     1201 ttataaatgg tgcgaattgt ggcttgctgt gagggacaga tgaatttact aaagtttatt
     1261 aagtatgtat tgaaagcgag agcaataatt tgcctgatcc ttatgtttat tattgtaact
     1321 gctatttttt atcagcgggg ggggcttgct tcggattcca tggatagtcg cataacaaag
     1381 attgtttatc aggcgcatgc ttttgttctt gaaaacaact gtgatttatc tatcgcacaa
     1441 aaagccttcg agacagatcc gcgttttatt gataatcaaa aaatgatcta tatttttatg
     1501 cactcatacg atactgtgcg aaaagaagtt atatgtatag ctcagggggc acgtcccgag
     1561 ttagttggta aaaacatgtg gtcgttacgt acgccagagg ggcgattgat atttcaagaa
     1621 agcataacgc tcttgaaagg caaagaagag ttttggcttg aatatgattg gctcaaccca
     1681 tacacaaatc ttgtgaaaac caaaagagca ttttataaaa aaattgtttt gtcagatgga
     1741 cgtaaagcat gggtgggcag tggatactgg aaaaactgat taggctgtag agagaccttt
     1801 tatgcttatt aaaacaaagc tttggctagg tggagccata atacttgtaa tacttatttt
     1861 tctggcttcc ttgaatatag taatgcataa gaatatttta gtcagccttg atgctcgtga
     1921 tcaggtgcgc ggtacacttc acaatgctga agagtataag aagtggcgtc aggatgtcga
     1981 tgcatttgtg acaaaaatgg tctcgcaaaa cgctgttcct gcaatgccac gaaatttttt
     2041 attactacct tatgatcagc agacagccga aacggatgag tttattgaat cagtcaaaaa
     2101 tttaataaag ctcgttgatc aatatgaaag tgctgtaaag agtaatgaaa gactcttcga
     2161 agaacatcga tcaaacataa tatccctcta caaaaagctt gatcataaaa tcgctactgt
     2221 tcttgctcaa ctgcagatgc gagaggttct cggtgaagat gtctcgaatg aatcttctct
     2281 ggcaccgtat gtgcttaaga gtttgaatca actgacactc attgctttaa acagtctggt
     2341 gacccgacag taccctgacg aaaacaaaaa aactgtgaaa tttaaccatc gttttcttga
     2401 tatgcagttg aaaattattg attcagatgg gtcaatagca gcattattca aaaaactgtt
     2461 tattcaaatt gattctatcc aaaaattgat tttggcatct tctgcaaatg aactcaaact
     2521 gagcgcgcaa ataggtgaag ctaaaaaaaa ttatgatagg gcttatctgc tttcaagtca
     2581 ggagtatcgt cttcaccgtg ctcagtcaag agtcgaaaag gctgatcaca atttacaact
     2641 gattagtagc cgcacccttc ttttaacctc tgtttttctg atactggtac ctcttctggt
     2701 aattggtttg gctcttttgg gtgtaaaccg gcttatattc ttaccagtta aaaatcttgt
     2761 aaagataatc agaaaatttg aaaaaggcga tcttttggca atcgcgaaga taagatcgga
     2821 tgatgagata ggaatgttat cttcagcatt taacaagatg gccgaacaga ttcgtcaaaa
     2881 ggtaagtgat ctggatttac ttaacaggga actgggtgag agcgaagaga aatatcggct
     2941 gttagcaata aactcggaag acgtaatttt tacattaaat aaagatctgc aatttaccta
     3001 tattagccca tcaatcaatc gtcttagggg gtccaaagag tcagattata taggaaaaaa
     3061 attcagtgat aaagttccgg cagaagcctt ggacgaagtt atgaaaataa cgaaacagct
     3121 tcaaaagcgg ataaagaccg gatcgaataa taactcggga atcaagcgtt ttctattaaa
     3181 attaaagggt aaaaatggta aaagtttttg ggtcgagatt accatgtctc ttatgtatgg
     3241 taaagggaag aaggttttcg gacttcttgg tgtagcaaga gatattacag atcgggtaag
     3301 ggccgaagaa aaacttaaga cctctttggg ggaaaaggaa attctgctta gagaggtgtt
     3361 acaccgaaca aagaacaaca tgggagttat ctcctctttt ctggaattgc aatctgcatc
     3421 gtataaaagt gaagaggtaa agcatcttgt taatgatagt acggcacgaa ttaaaaccat
     3481 ggcattggct catgaaatgc tctataacgg taaagatata tcaaaaataa atatgcgcga
     3541 ctatattaca ggacttatgg agcttcttga aaagagtaat cgatttgaat cggacagagt
     3601 atcgt
//
LOCUS       DAOTGZ010000266         3593 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_3064
            NODE_30684_length_3593_cov_3.842849, whole genome shotgun sequence.
ACCESSION   DAOTGZ010000266 DAOTGZ010000000
VERSION     DAOTGZ010000266.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868088
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 3593)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3593)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:22:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,785
            CDSs (total)                      :: 2,751
            Genes (coding)                    :: 2,732
            CDSs (with protein)               :: 2,732
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 12 of 19
            Pseudo Genes (internal stop)      :: 5 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3593
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_30684_length_3593_cov_3.842849"
                     /isolate="AMR_MDS_3064"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..860)
                     /locus_tag="P5123_12645"
     CDS             complement(<1..860)
                     /locus_tag="P5123_12645"
                     /inference="COORDINATES: protein motif:HMM:NF019310.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HRX17157.1"
                     /translation="MNSKKYLLVIPLSFIAFISLGLPDGLMGVAWPGISKEFSVPLDY
                     AGILLLTTMGGYIVSSFFSPVITRKLGVGGLLAASCALTATGLFFYGVSSFWIIFILF
                     SIFLGIGAGAIDAGINTYIAQNHSERLMHWLHASFGIGITIGPLIMTYGIRFTGTWRT
                     GYYITAVAQFILAIIFFIKRSQWEKPTTDITEQNRTEKKDAPITETLKLLPAYLSMLL
                     FLLYTGVELGFGFWIFTLLTEARGIDLQTAGIVTSLYWGSFTAGRVIAGLITKAISGT
                     KLLVLAMITA"
     gene            1669..2223
                     /locus_tag="P5123_12650"
     CDS             1669..2223
                     /locus_tag="P5123_12650"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRX17158.1"
                     /translation="MIKINWPIAAIVTCFISLTGYIVLNDVNYRRTSLYRDVQMNTYI
                     VYSDWHLFVGTLKGLTLTTYGFADTLNEAEQLKKHIETKIKLLEKDSSDLDPQIGEPL
                     EAFLSSIEAGLRLGQQLIDNGYLFLDQPDLPLVFKEGRAGLSGLAGKDATEHMGTLSA
                     YQYYQLIGRLRGMNSLLIRSIRID"
     gene            2232..3167
                     /locus_tag="P5123_12655"
     CDS             2232..3167
                     /locus_tag="P5123_12655"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HRX17159.1"
                     /translation="MANIASRSARLQYNFFILRIILLLFTSIIIILTIFRLYILNRSL
                     KSLADKTSKELDSTRTHLSEVQNYLHNAQFQQSLFQMVAGLSHELNTPLGNCVSLSSY
                     LQSVFSSFKSCTNDGNITKDQFLHDVNKGIEGFELMRDNLEQMKTQIETFKRLASVNH
                     QSSGGKVLLSDYLDHYLQAEVNRKLPGIAAVINSEGCNDLSVRVADLDQIFEQLLNNT
                     AEHTSADKVNISFIKSDDILKIDYYDNGSGVSSDNLSRLPEPFFTTARGKKHMGLGLS
                     IVSSLVNNKLQGSIDFSATGHGLEITIIIQVSAIM"
     gene            complement(3183..>3593)
                     /locus_tag="P5123_12660"
     CDS             complement(3183..>3593)
                     /locus_tag="P5123_12660"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HRX17160.1"
                     /translation="ALSHLMMFAPFPSVKNGKGSIKDAMGGCNGFVIGKNAPDEAIEF
                     LEFFTRAENLQKSFDVFPAIPTVNEVVIKNEGHKAVKKYLNTMSSFNLYPDQLFPLET
                     GSMINEVSARIMLGELTPEEGCKILDRTWEASKD"
BASE COUNT         1144 a          632 c          666 g         1151 t
ORIGIN      
        1 attgccgtga tcattgccaa aactaagagt ttcgttccac tgatagcttt tgttattaac
       61 ccagctataa ctcttcctgc agtaaatgat ccccagtaaa gagatgtaac tatacccgct
      121 gtctgtaaat ctatacctcg agcttcggta agcaaggtga atatccagaa tccgaatcca
      181 agctctacac ctgtatataa caagaacaga agcatactta agtatgcagg taataatttt
      241 aaagtctctg taatgggtgc gtcttttttt tcggttctat tttgttctgt tatgtcagta
      301 gtaggttttt cccattgtga tctttttata aagaaaataa ttgcgagtat aaattgagcc
      361 acagcggtaa tatagtatcc tgtgcgccag gtacctgtaa agcgtatacc gtaagtcatt
      421 atcaatggac caattgttat tcctatacca aagcttgcgt gaagccagtg cataagtctt
      481 tcactgtgat tttgtgcaat ataagtgtta attccggcat caatggctcc ggctcctatt
      541 ccaagaaaaa tcgaaaagag aataaaaatg atccaaaaag aagaaacacc ataaaagaaa
      601 agtccggtag cagttaatgc acagcttgca gcaagcaatc ctccgactcc aagctttcgg
      661 gtgataacgg ggctaaagaa gctcgataca atgtatccac ccattgtagt taaaagtaaa
      721 attccggcat agtcaagtgg aacactaaat tcctttgaaa tgcctggcca agcaaccccc
      781 ataagaccgt cgggtaggcc caaagaaata aacgcgataa atgatagagg tataaccaga
      841 agatattttt tactgttcat gtgaaaccgt tactggatat ttataaagat agaaataaaa
      901 atgtttgaac ctgtttttca taaatcgtat atttgtaaag tccaaaaaga ggtaaaatga
      961 aattgtgttc atttgttatc aaattcaccc tacttaaaat tattataact aaaaaattga
     1021 acagtagtaa gaatatcagt gagctatgat ctgtatatag aacgatattt attattatag
     1081 cataatgcgg tgtaatataa atattaaatc gataaattgc gaaatatcta ggtcatcata
     1141 acatggatat ttcgctgtat ggggatagtt aatacttgct tataattata atgttccaga
     1201 gggaaacttg gcatagttgc ctattctaaa gaaaatttcc gcaagttcct ttgctgaaaa
     1261 tttaatttta atatgttacc atatttttaa aattataaca atatccaagt aattacagca
     1321 gtttttgaag ttgcactgtt gtttctgctc caactttagt attataagct aatttttttt
     1381 taaccaattt tacgttaaca taagactaaa gtttgagatt aatacaaatc ttgcattagt
     1441 gcatacttgg ttgtcattat ttcaattatt cacattgaat gttccttggt tatgaaaaaa
     1501 aatttaaaat tatttgcttg taaattatat agtaaacata acagttttga ttgagtttta
     1561 tctttttgat agtgtggtaa ttaaatagga tatggtttgt tgacaagtgt tgctaataac
     1621 tgtaaatata gagttagttg tttgataaaa taatcaggat gaaactgaat gattaagata
     1681 aattggccta ttgccgctat tgttacctgt tttattagtt taaccggata tattgtttta
     1741 aacgacgtta attatcgcag aacctcgcta tatagggatg ttcaaatgaa tacctatatt
     1801 gtctattcgg attggcacct ttttgttgga accctcaaag ggcttacgct aaccacatac
     1861 ggttttgctg atacgttaaa cgaagccgaa cagcttaaaa aacatatcga aacaaaaatt
     1921 aaattattag agaaagattc ttctgatctt gacccgcaaa ttggtgaacc gcttgaagcg
     1981 tttttaagca gtattgaggc cggattgcgt ttggggcagc aattaattga caatggttat
     2041 cttttcttag atcagcctga tcttcccctt gtcttcaagg agggtagggc cggactttcg
     2101 ggtttggccg gaaaagacgc gacagaacat atgggaactt tgtctgctta tcaatattac
     2161 cagcttatag ggcgcctgcg cgggatgaat tcacttttga tcagatctat tcggatagac
     2221 taaatgagct tttggccaat atcgcttcac gttcagcgag actacaatat aactttttca
     2281 ttttaagaat tatactgctg ctttttacat cgattataat aatattaacc attttccggt
     2341 tatacatact aaatcgttct ttaaaaagcc ttgctgataa gacatccaaa gagctggaca
     2401 gtactcgtac tcatctatcc gaggttcaaa attatctgca taatgcacag tttcaacaat
     2461 cactttttca gatggtggcc ggattgtcac acgaacttaa tactcctctt ggtaattgtg
     2521 tgtctctgtc tagctatctt caatccgttt tttcgagttt taagagctgt acgaacgatg
     2581 gtaatattac aaaggatcag tttttacacg atgtaaataa gggtatcgaa gggtttgaac
     2641 ttatgcgtga caatcttgaa cagatgaaaa cgcagataga aacattcaag cggcttgcga
     2701 gtgttaatca ccaatcatca ggtggtaaag ttttgttgtc cgattatctt gatcactatt
     2761 tacaggcaga agttaatcga aaactgcctg gtatagctgc ggtgataaat tcagaaggat
     2821 gcaacgatct tagtgtgagg gttgccgacc ttgatcagat atttgaacag cttcttaata
     2881 atacagcaga gcacacatcg gcggataagg tcaacatcag ctttattaaa tctgatgata
     2941 ttttgaaaat agattattac gataacggtt ccggagtttc aagtgataac ttatcaaggc
     3001 ttccggagcc cttttttacc accgcaaggg gaaaaaaaca tatggggctt ggactttcta
     3061 tcgtctcatc acttgtaaat aataaacttc agggaagtat cgatttctct gcaaccggcc
     3121 atgggcttga aatcacaatc attattcagg tctcagcaat catgtaataa tgtaattaaa
     3181 aatcaatctt tagaggcttc ccacgttcta tccagtattt tacacccctc ttctggggta
     3241 agctcgccga gcatgatccg tgcggagact tcattaatca ttgaacctgt ctcaagcgga
     3301 aaaagctgat ccggataaag attaaagctg ctcatggtat taaggtattt ttttactgcc
     3361 ttatgcccct cgttttttat aacaacttcg ttaacggttg gtattgccgg aaatacatca
     3421 aaagatttct gaagattttc cgcgcgagta aaaaattcaa gaaattctat tgcttcatca
     3481 ggcgcatttt ttccgataac aaatccgtta caaccgccca tagcatcctt gattgatcct
     3541 ttaccatttt taacagaagg aaatggagca aacatcatta gatgagaaag agc
//
LOCUS       DAOTGZ010000267         3566 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_3064
            NODE_31002_length_3566_cov_6.174879, whole genome shotgun sequence.
ACCESSION   DAOTGZ010000267 DAOTGZ010000000
VERSION     DAOTGZ010000267.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868088
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 3566)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3566)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:22:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,785
            CDSs (total)                      :: 2,751
            Genes (coding)                    :: 2,732
            CDSs (with protein)               :: 2,732
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 12 of 19
            Pseudo Genes (internal stop)      :: 5 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3566
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_31002_length_3566_cov_6.174879"
                     /isolate="AMR_MDS_3064"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..416
                     /locus_tag="P5123_12665"
     CDS             <1..416
                     /locus_tag="P5123_12665"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRX17161.1"
                     /translation="GMPDYNRVNDKDYMNKYYNRTVYRETSGQYSVQRHTVMLYNRWE
                     LLLNRIKIKTGIGYQFSHGLAKESGMGIGAAIGELKALFTLQDALLLEGGIHQCLPLE
                     YSRDSGNFHKDRESELYPLYEANYIYFMLSWKWSR"
     gene            469..1149
                     /locus_tag="P5123_12670"
     CDS             469..1149
                     /locus_tag="P5123_12670"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRX17162.1"
                     /translation="MKTITKTAAIVFSILVVAVLHAEPKIKDGETFTYINTVNGVESR
                     AVCTYHNHANGYSYKYVSDRETWTASTDKKGNPSSVTHETQGNEIHFDYNGKSVTMHG
                     MWNGTTVNESKQFEIRVTSENALLLRTNNFTKNPEITFEMQQPSDYPYLNTIKMKYSF
                     EGIEEVTVQAGTFKCKKIKFSPVGFKAFFFSAYYYITDDDRQIIVKSENMPVNGQSEL
                     VKITTSQE"
     gene            1230..>3566
                     /locus_tag="P5123_12675"
     CDS             1230..>3566
                     /locus_tag="P5123_12675"
                     /inference="COORDINATES: protein motif:HMM:NF014513.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MMPL family transporter"
                     /protein_id="HRX17163.1"
                     /translation="MKKSDKNPTSKRASAADHVFNLLIKLRFIMIALSVVLFFYFGFH
                     MKFITQNSSAKIDSVLAENDKVLQQYNQTKEIFPLTVGQEKLFIVFETESVYSNHFLI
                     FLNDAINSVSEIDGVNKCNGIFSTYNISGEDNELVISPFAKTENLPFSESQLKELRAK
                     IEGNPMTAGRLANSEATCTTVILSAESGLNDEKKQCLYNSVQNILYQKKISSPGNEKI
                     SIHYAGDMVIQQEISLIQSEEKHIFPIMILTVIIILFAVFRTLAGVLIPVSIMIISVT
                     TTIGVKALCGSSMSAIDPMLYALITTISVADSIHFVSSYYNPVLFPLLKKRERIIQVM
                     RHSIYPCFLTSLTTAIGFFAITLSPITQLKSFGLYASTGVMISFAYTVILVPGALCFF
                     DFSVIRHRFVKTNRAWDNLLSRIIDYLSYCAINKYKKTILIFGIIFILSAFFTVFLKT
                     GTNPYSFLKDSHRVNKALSVIEKNFGGIDSIDIIIQSENPDVFKQKKIISQLDHLINN
                     LSKTKGIEHIESFVPLLKQMNSAIYSKQDQSYRLPDKDELIPQYFLLYEMSDDSETLY
                     TWVNSDYSTARIRLTPGSDSNIRELTDQINTVIKESGLDSELDIVITGSAVLWNHVDS
                     QFIKGQLLSLLFSISLITALLFLIFRSVRLGLCAVAVNIFPIIFGLGFMGLFGIKLSM
                     GTVMIASIAIGIAVDDTIHTIFKYKILSSQGEPFNTNIHKVFGALFRPVVFTSLILAA
                     GFLSNTMSAFKPNAWFGLISAIVIIIAMLADIFLLPALL"
BASE COUNT         1140 a          724 c          658 g         1044 t
ORIGIN      
        1 acggcatgcc cgattacaac cgcgtaaacg acaaagacta tatgaacaag tattataatc
       61 gaactgttta ccgggaaaca tccggtcaat acagcgtaca acgtcataca gttatgcttt
      121 ataaccgatg ggaactgttg ttaaatcgca taaagataaa gacaggtatc gggtaccagt
      181 tttcacacgg ccttgcaaaa gagtcaggta tgggtattgg cgcagctata ggcgaattaa
      241 aggctctgtt cactttacag gatgctcttt tactggaagg agggatccac caatgcctgc
      301 ctcttgagta cagccgggat tcaggtaatt ttcacaaaga ccgcgaatcc gaattgtacc
      361 ctttatacga agcaaattac atatacttta tgctaagctg gaaatggtcc cgctaagata
      421 taagtatttg ttttgataag attaaaagta taaaaaaagg agatcaaaat gaaaaccatt
      481 acaaaaacag cagctatagt gttctcaatt ttagtcgtag ccgttttaca cgcagagcca
      541 aaaatcaaag acggtgaaac cttcacctac ataaacacgg taaacggcgt cgaaagccgc
      601 gcggtatgca cataccataa ccacgcaaac ggttattcgt ataaatatgt atcggacaga
      661 gaaacatgga ctgcctctac tgataaaaaa ggaaacccct cgtctgtaac acacgagaca
      721 cagggaaacg agatacattt cgattataat ggcaaatccg taaccatgca tggaatgtgg
      781 aacggaacaa ccgttaacga atcaaaacag tttgaaattc gagtaacttc agaaaacgcc
      841 ctgcttttaa gaactaataa ttttaccaag aatcccgaaa taactttcga gatgcagcag
      901 ccttctgact atccatacct taacacaata aaaatgaaat actcttttga aggaatagaa
      961 gaggtaactg ttcaggcagg aacttttaaa tgtaaaaaaa tcaaattcag tcctgttgga
     1021 ttcaaagcct ttttcttttc ggcatattat tacataaccg atgatgatcg gcagataata
     1081 gtaaaatctg agaacatgcc ggtaaacgga caaagcgagc ttgtcaagat aacaacttca
     1141 caagaataag gccagtcacc tgaaacctta gatacgtaat cgaacagcta ttacgcgata
     1201 tacggtaaaa ccaaagtatg gagcagacaa tgaaaaaatc agacaaaaac ccaactagca
     1261 aacgggcttc cgcagcggat catgttttta acttacttat taaattaagg tttatcatga
     1321 tagcactttc cgttgttctg ttcttttact ttggctttca catgaagttt atcacacaga
     1381 acagctctgc aaaaattgac tctgtactgg ctgagaacga taaggtttta caacagtata
     1441 atcagaccaa agaaattttt ccccttactg tcggccagga aaaacttttt atcgtattcg
     1501 agactgaatc tgtttactct aaccactttt tgattttttt aaacgacgcg attaattctg
     1561 ttagcgaaat cgatggcgtg aataaatgta acggaatttt ctctacatac aatattagcg
     1621 gagaagataa tgaactggtg ataagccctt ttgccaaaac tgaaaatctg ccatttagcg
     1681 aatcacaatt aaaagaacta cgcgcaaaaa tcgaaggtaa tcctatgaca gccggaaggc
     1741 tggcaaacag cgaagctaca tgtaccaccg taattttatc agcagaaagc ggtctaaacg
     1801 acgaaaaaaa acaatgtctc tataactcag tacaaaatat cctgtatcag aaaaagataa
     1861 gttctcccgg taatgaaaag ataagcatac actatgcagg agacatggta atacaacagg
     1921 agatctcgct tattcagtca gaagaaaaac atatttttcc gataatgata ctgaccgtta
     1981 taatcatact ttttgccgtt tttcgcactc ttgcaggggt tctgattcct gtttcaataa
     2041 tgattatatc agtaactacc actattggag taaaggcttt atgcggttca tccatgtccg
     2101 cgatagatcc gatgctctat gctttgatta ccaccatatc agttgccgat tcgatacatt
     2161 ttgtatcttc atattataat cctgtattat ttccgttatt aaaaaaacgc gaacgtatta
     2221 tccaggtaat gcggcattca atttatccct gttttttaac ttctcttacc actgctattg
     2281 gcttttttgc cattacctta agtcccataa cccagttaaa gagttttggt ctgtacgcgt
     2341 ccacaggtgt tatgatctcg tttgcgtaca cagtcattct ggtgcccggt gcactctgct
     2401 tctttgattt ttcggttatt cgtcaccggt ttgtaaaaac aaacagagca tgggataatc
     2461 tgttatccag aatcattgat tatttatcat attgtgccat taataaatac aaaaaaacaa
     2521 ttcttatttt cggtatcata tttatcttgt ccgccttctt tacagttttt ttaaagaccg
     2581 gaaccaaccc ctactcgttc ttaaaggatt cgcacagggt aaacaaggct ctttctgtta
     2641 ttgaaaaaaa ctttggcggt atcgattcaa tagatattat tattcaatct gaaaatccgg
     2701 atgtttttaa gcagaaaaaa attatcagtc aattagatca cctgataaat aatctttcaa
     2761 aaacaaaggg aattgagcac atcgaaagtt ttgtgccctt gttaaagcag atgaacagcg
     2821 caatttacag caaacaggat cagtcgtacc gcctgcctga taaagacgaa ctgattccgc
     2881 aatatttttt gctatacgaa atgtccgatg actcggaaac actttacacc tgggtaaaca
     2941 gcgattactc tacggcaagg attcgtctta ctcccggttc tgattctaat atcagagagc
     3001 tgacagatca gataaacact gtaataaaag aaagtggttt agacagtgag ctcgatattg
     3061 taattacagg atctgctgtt ctctggaatc atgtcgactc gcagtttatt aaaggacagc
     3121 ttttgagcct tctgttttca atctctctta ttacggcttt gcttttcctt attttcagat
     3181 cagtcagact gggactctgc gccgttgcag taaatatttt tccgataatt ttcggactgg
     3241 gatttatggg actgttcggc attaaactca gcatgggcac cgttatgatc gcttctattg
     3301 caatcggcat tgcggtagat gatacaatac atactatctt taaatacaag attctaagtt
     3361 ctcagggaga accttttaac accaacatac ataaggtttt tggtgcgctt ttcagaccgg
     3421 tggtttttac atctttgatc cttgcggccg gatttttatc caacaccatg tcggcgttca
     3481 aacctaacgc ctggtttgga ctcatctcgg caattgtaat tataatagcc atgcttgccg
     3541 acatcttttt actgccggca ctgctc
//
LOCUS       DAOTGZ010000268         3494 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_3064
            NODE_31826_length_3494_cov_6.064263, whole genome shotgun sequence.
ACCESSION   DAOTGZ010000268 DAOTGZ010000000
VERSION     DAOTGZ010000268.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868088
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 3494)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3494)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:22:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,785
            CDSs (total)                      :: 2,751
            Genes (coding)                    :: 2,732
            CDSs (with protein)               :: 2,732
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 12 of 19
            Pseudo Genes (internal stop)      :: 5 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3494
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_31826_length_3494_cov_6.064263"
                     /isolate="AMR_MDS_3064"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..762
                     /locus_tag="P5123_12680"
     CDS             <1..762
                     /locus_tag="P5123_12680"
                     /inference="COORDINATES: protein motif:HMM:NF018004.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1015 family protein"
                     /protein_id="HRX17164.1"
                     /translation="KKEIRELFEDVLKNEPLYEFTSNDGVYHALRAIRADDQISSLVE
                     YFDCLDLYIADGHHRAASAARVGRERAAKGIKDGSDYFLSVVFPDEQLYIMPYNRVVN
                     DLNSHSKDEFFELLSKKYIIEATNNKTPLASSSVAMYIDSEWYMLTPLFEISSDPVES
                     LDVQILQKQVLSPILGIDDPRVSERIDFVGGIRGTDELERLVNSGNFAVAFSMYPTTI
                     DQLISVSDNGLVMPPKSTWFEPKLRSGMVIHAFKK"
     gene            769..1068
                     /locus_tag="P5123_12685"
     CDS             769..1068
                     /locus_tag="P5123_12685"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRX17165.1"
                     /translation="MEQGELNNRLQKLEDENRQLLEVIESYKQVLRLNEKELATQMAI
                     VSMYESAIEYSRVEMIDFKETISANERVAQLCDEEKKQVLAKIRELTDNKFKIKT"
     gene            1087..2445
                     /locus_tag="P5123_12690"
     CDS             1087..2445
                     /locus_tag="P5123_12690"
                     /inference="COORDINATES: protein motif:HMM:NF016362.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HRX17166.1"
                     /translation="MAYISYIIVFLIIIVVTILALYVLSTYFSPRKLDEIAEMIKKGQ
                     FKLAIRKLDEILANDDRNVYAHYLLAEAYRLDKNPQYAILEFRQVVKLGTYNDKVNEV
                     DVREKLALIYKEQNKINDAKKEYMILTQIDPNNYLNYYELGLLFFNASVLDKALDYLK
                     KAASLHQTRSDIFYYIGQIQYRSGNNKDAKNAFINTIKLDASNTKAHYFLGLVLRSEG
                     DVEWAVKEFEIAQRSEDIRSKCFLAKGSCYLETEQYPKAIIEFDKGLRTVRRGSDTEL
                     NMRYFLAAAHEKMRDLQTAIEHWEKIYEQKKNFRDVEQKLRQNAEFRQDDRIKDFMIA
                     SLSNFEHLSRKMVEVMNFEVQELKVLRDTEIEIYAVEKEDNRRNTRRVYRIIRIYRTT
                     NVINDGYMRNLYDMMKPKNAQRVMVISTGEFAQSAIDYSNTRPIELVTKTKLIELLKK
                     VS"
     gene            2445..>3494
                     /locus_tag="P5123_12695"
     CDS             2445..>3494
                     /locus_tag="P5123_12695"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRX17167.1"
                     /translation="MSFSGFANNHLFSFLFKHEEKEFIDCFEPILLKKGAKLTIEPSN
                     IFYYYIESGSVSVSFENGNQTLVMHSGDGFGRLPFAVTSDRYSINVLEESLVFRIDDE
                     RMLLYLLSDLKKIRGYLGAVVNSGIEIINDELVSGLSDTAVIGVVGEVKSGKTLFSAL
                     IAKNLSEKGRVLLIDASRSHSGLSALLGCNSGVPVVIKEQNKEFDYQKYLVEVNSNLS
                     LINFSDGEKTDNSNNLLAALIVNTSFEFDYIVIDGCPASISEYVLANCDCIFNMCRNG
                     NDKRSAGIWLDRFICEGQRIVHVANRLFLDNSDGYSVNLDHITDISRSGFEEATKNIR
                     VDDAVKFIDKKNINFF"
BASE COUNT         1118 a          558 c          764 g         1054 t
ORIGIN      
        1 aaaaaagaga ttcgcgagct gtttgaagat gtgttaaaaa atgaaccact ttacgagttt
       61 acatcaaatg acggggttta ccatgcgttg cgagcaatta gagcggatga tcagatctcg
      121 tcattagttg aatattttga ttgtctggat ctttatatcg ccgacggtca tcaccgtgct
      181 gcttccgctg cacgggtagg tcgtgagaga gccgcaaagg gaattaaaga tgggtctgac
      241 tatttcttgt cagtggtttt ccccgatgag caactttata taatgccgta taacagggtt
      301 gtaaatgatc tgaattctca ttcgaaagat gaatttttcg aacttctttc aaaaaaatat
      361 ataatagaag caacaaataa caaaactcct cttgcttcgt cctctgttgc gatgtatatt
      421 gattctgaat ggtatatgct aacaccgctt tttgaaatat catctgatcc ggtagagtct
      481 cttgatgtgc agatactgca aaaacaggtt ctttctccta ttcttggtat tgatgatccg
      541 agagtttccg aaagaattga ttttgttgga ggaatacgag gaacagatga gcttgagagg
      601 cttgttaatt cgggtaattt tgctgtggct ttttcgatgt atccaacgac cattgatcag
      661 ctgatatcag tatcagacaa tggattggtt atgccgccaa aatcaacctg gtttgaacca
      721 aaactacgca gcggtatggt tatacacgct tttaaaaagt gaggaaacat ggaacaggga
      781 gaacttaata accgtttaca gaaacttgag gatgaaaatc gtcaacttct tgaggtaatt
      841 gaaagttaca agcaggtact acggcttaat gaaaaggaac ttgctacaca gatggccatt
      901 gtttcaatgt atgaaagtgc cattgagtac tcccgggttg aaatgatcga ttttaaagag
      961 actatttctg caaatgaacg cgttgctcaa ctttgtgacg aagaaaaaaa acaggttctt
     1021 gccaaaatca gggaacttac cgataataag tttaagatta aaacctgata aggaactgag
     1081 ttttcaatgg cctatatctc ctatataata gttttcctga taataattgt tgtaacgatt
     1141 cttgcgttat acgttctttc aacatatttt tctccccgaa agcttgatga aattgccgag
     1201 atgataaaaa aagggcaatt caagctggca atacgtaaac tcgatgaaat tcttgccaat
     1261 gatgacagaa atgtttatgc ccactatctt cttgccgaag cataccgctt ggacaaaaac
     1321 cctcaatacg cgattctgga atttcgacaa gtagttaagc ttggaacata taatgacaag
     1381 gttaacgaag tcgatgtaag ggaaaaactt gcattaatat acaaagaaca gaataaaata
     1441 aacgatgcaa aaaaagagta tatgattctg actcagattg atccgaataa ttatttgaac
     1501 tattatgagc tggggcttct cttctttaat gccagtgtgc ttgataaggc gctggattat
     1561 cttaaaaaag ccgcaagcct tcatcagact aggtcggata tattttatta tattggtcag
     1621 atacaatatc gctcaggaaa caataaggat gcaaaaaatg cctttattaa tacaattaaa
     1681 cttgatgcat ctaatactaa agcacactat tttctgggac ttgtattgag aagtgaaggc
     1741 gatgtcgagt gggctgtaaa agagtttgag atagcgcaaa gaagcgaaga tattaggtca
     1801 aaatgttttt tggctaaagg aagctgttac ctagagacag aacagtatcc gaaggcaata
     1861 atcgagtttg ataagggatt gcgaactgta aggcgaggat ctgataccga acttaatatg
     1921 agatattttt tggcagcggc acatgagaag atgcgtgatc tgcagactgc aatagagcat
     1981 tgggaaaaga tttacgagca aaagaaaaat tttagagatg tagagcagaa acttagacag
     2041 aacgcggagt ttaggcagga tgacagaata aaggatttta tgatagcgtc actttccaac
     2101 tttgaacacc tttctcgcaa aatggtagag gtaatgaatt tcgaagttca ggaactgaag
     2161 gtactcaggg acactgaaat tgagatttat gctgttgaaa aagaggataa tcgtcgcaat
     2221 actcggcgag tttacagaat aatcagaatt tatagaacga cgaatgtaat taatgacggt
     2281 tatatgcgaa atctttatga tatgatgaaa ccaaaaaatg cgcagcgggt tatggtaatt
     2341 tcgacgggtg aattcgcgca aagtgccatt gattattcaa atacacgccc aattgaactt
     2401 gttaccaaga caaaactgat agaactgctt aaaaaagtgt cctgatgagt ttttccggat
     2461 ttgcaaacaa tcatcttttc tcttttcttt ttaaacacga ggagaaagag tttatcgact
     2521 gcttcgagcc tattttgtta aaaaaaggtg ccaagcttac tatcgaacct tccaatattt
     2581 tctattatta tatcgagtcg ggcagtgtct cggtttcttt cgaaaacgga aatcagactc
     2641 ttgttatgca ttcaggagac ggttttggac gtctcccatt tgccgtaacc agtgacaggt
     2701 attcgattaa tgtattggaa gagagtctgg ttttcagaat cgatgatgag agaatgctgt
     2761 tgtatctttt atccgatcta aaaaaaatca gggggtatct tggtgccgta gtaaatagtg
     2821 gaattgaaat aattaacgat gaactggttt ccggtctttc agatacagct gtaattggcg
     2881 tcgtgggcga ggttaagagc ggtaaaacac ttttttctgc actgattgcg aagaatctgt
     2941 ccgaaaaggg aagagtatta ctgattgacg cttctaggtc gcatagcggt ttatctgctc
     3001 ttttaggctg taacagtggc gttcctgtag taatcaaaga gcaaaacaag gaatttgatt
     3061 atcagaagta tttagtggag gtgaattcaa acctctcttt aatcaatttt tcagatgggg
     3121 aaaaaactga taacagtaac aatctgctcg ctgctctgat agtgaatact tcatttgagt
     3181 ttgattatat cgttattgac ggttgcccgg cttctatttc cgaatatgtt ttggctaatt
     3241 gcgactgtat tttcaatatg tgtcgtaatg gtaatgacaa aagatccgct ggtatctggc
     3301 ttgacagatt tatttgtgaa ggtcagcgaa ttgtgcatgt ggcaaacagg ttgtttttgg
     3361 ataatagcga tggatattct gtaaatttgg atcatattac agatatttca agatctggtt
     3421 ttgaagaggc aacaaaaaat ataagagtag acgatgctgt caaatttatt gataaaaaga
     3481 atattaattt tttt
//
LOCUS       DAOTGZ010000269         3458 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_3064
            NODE_32269_length_3458_cov_4.704085, whole genome shotgun sequence.
ACCESSION   DAOTGZ010000269 DAOTGZ010000000
VERSION     DAOTGZ010000269.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868088
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 3458)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3458)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:22:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,785
            CDSs (total)                      :: 2,751
            Genes (coding)                    :: 2,732
            CDSs (with protein)               :: 2,732
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 12 of 19
            Pseudo Genes (internal stop)      :: 5 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3458
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_32269_length_3458_cov_4.704085"
                     /isolate="AMR_MDS_3064"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(65..2176)
                     /locus_tag="P5123_12700"
     CDS             complement(65..2176)
                     /locus_tag="P5123_12700"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="7TM-DISM domain-containing protein"
                     /protein_id="HRX17168.1"
                     /translation="MPRLLMAAILLITIVGCTPSSRTEQPAIRNGILDLALWDRDRSP
                     VINLSGNWIFYDNYLLYPSDRSKSDSDHVYATVPGLWNRALTKKRASGTGYATYKLII
                     MAPDNKKELAIKFGSIRSSYICWLNGQFVNNGGVVGSTETSSIPQFSTKLVPVSLQKG
                     TNELIIQVSNYHDNQGGFWDPLYFGDYGNLNRDLITSLSQIFILVGFLAFAVLFLFVI
                     GFFTNSNRDLIPLILFAILLAIRILLEREHFLYNLMGNKVWPVLFKAEYLSGVLALTS
                     VFFYLMNSLLKIKNVKTHLFLTPMYITCMVIIFSSSLFFSKTLNIIYFYMVLQSILIL
                     LTLTIRVKRKSNTIADLLFTSVVVISVGYYTILRILFNESSVAFYFILSLYIFYLIAT
                     RYITLFKSYNNSRSDNIQTTNRYIYGKRLYSSGLLDLFRKPVENLKSGDSIMLDGTLL
                     LLIFSRETSNVFSQSEEQEWFVQLNSVYASITSYIKEHNGIPVYYRDNSMLILFTGTV
                     IEAYKSALNAFDFIQKYNGKGIQISEQSIKSRIVMYKSRFVIALAGSDDTLQPLITGD
                     RIKKMYDSEKIGKDLEAKFIINREIFYYLVADTDSRCPLFFRLLGQATTNLNENGEAI
                     YEIFEHIDSETRDLRESTKDFFENGVCNYMLGKLEKAEQQFAKTINMDPNDRTAQIYL
                     EKVKNRKKISVYEKQYWGEET"
     gene            complement(2257..>3458)
                     /locus_tag="P5123_12705"
     CDS             complement(2257..>3458)
                     /locus_tag="P5123_12705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004812803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="helicase-related protein"
                     /protein_id="HRX17169.1"
                     /translation="LAFFQSKGKLVEAQRLESRTRYDIEMMTEMGYCSGIENYSRIIS
                     RRQPGERPDCLLDYFKGDYMMFVDESHVTLPQVRGMYHGDRSRKQNLVDYGFRLPCAL
                     DNRPLYFEEFENIIDQALFISATPSDYELEKSAIIGEQVIRPTGLVDPIIEVRPATNQ
                     VDDLIGEINKRVEKKERVLVTTLTKKMSEELTTYLQDSGIACRYLHSEIETIERVEII
                     RDLRKGEFDVLIGINLLREGLDIPEVSLVAILDADKEGFLRSARSLIQTAGRAARNIN
                     GYVIFYADKITDSMKKAMDETERRRVIQTEYNSSNNISPKTISKDIYDIIEREYSTVN
                     MEETVVSEFKNRYRSNSIKELEELCTELKSDMLEAADKLEFEQAATLRDQMAKTEKKI
                     LMLKRST"
BASE COUNT         1023 a          666 c          675 g         1094 t
ORIGIN      
        1 tcgctgatgg tactgcatgg gtgaccgtgt gggagagtag gtcgctgccg ggatttaaat
       61 atattcatgt ctcttctccc caatactgct tttcataaac actgatcttc tttctatttt
      121 taaccttttc taaatatatt tgtgcggttc tgtcgtttgg atccatgttt attgtttttg
      181 caaattgctg ttctgctttt tcaagttttc caagcatata gttgcacact ccgttttcaa
      241 aaaaatcctt tgtagactct cttagatcgc gtgtttcaga atcaatgtgt tcaaagattt
      301 catatatagc ttctccgttt tcattcagat tagttgtagc ttgcccgagt aatctgaaaa
      361 ataacggaca tctggaatca gtatcagcga caaggtaata aaaaatctct ctattaataa
      421 taaattttgc ttccagatct tttcctattt tttccgagtc gtacatcttt tttattcgat
      481 caccggtaat aagtggctgt agggtatcgt ctgatccagc aagagcaata acgaatcggg
      541 atttatacat cacgattcga cttttaattg attgttccga tatttgaatc cctttcccgt
      601 tatatttttg tataaaatca aaggcgttta atgccgactt atatgcttct attacggttc
      661 cggtgaatag aatcaacatg ctgttgtctc tataatagac agggataccg ttatgttctt
      721 taatatagga agttatcgat gcataaaccg agttaagctg tacgaaccac tcctgttctt
      781 cagattgtga aaaaacgttg ctggtttccc tagaaaagat taaaagcagc agagtaccgt
      841 cgagcataat ggaatcaccg cttttcagat tctcgacggg ttttctgaag agatctaaaa
      901 gcccggacga gtagagacgc tttccataaa tgtacctgtt agtagtttga atattgtctg
      961 atctggagtt attgtaagat ttaaaaagag ttatatatcg agttgcaatc agataaaaga
     1021 tatacagact tagtataaaa tagaaagcca ctgaagattc attaaagaga attcgtaaga
     1081 ttgtgtagta ccccacggaa attacaacaa cagaggtaaa aagaagatca gctattgtat
     1141 tagattttct ttttacacga atagtcagcg taaggagtat tagtatggat tgtaaaacca
     1201 tatagaaata tattatgtta agagtttttg aaaaaaacaa tgaggatgaa aaaataatta
     1261 ccatacaagt tatatacatt ggagtcagaa aaagatgtgt ttttacattt ttaattttga
     1321 gtaagctatt cataagataa aagaataccg atgtaagggc aagaacacct gaaaggtact
     1381 cggctttgaa taaaaccggc cagaccttgt ttcccataag gttgtaaaga aaatgttctc
     1441 gttcgagtaa aatacggata gcaagtaaga tagcaaacag tatcagagga atcagatctc
     1501 ggtttgaatt tgtgaaaaaa ccaattacaa aaaggaaaag tactgcgaat gcaagaaacc
     1561 ctacaagaat aaaaatctgc gacaaactgg tgattaagtc tcgattaagg ttaccataat
     1621 ctccaaaata gagaggatcc caaaagccac cctgattgtc gtgatagtta gaaacctgaa
     1681 taattaattc gtttgttccc ttttgaagtg aaacaggtac aagtttagtc gaaaactgag
     1741 gtattgaact tgtctcagtc gaacctacaa cgccaccatt gtttacaaac tggccgttta
     1801 accaacagat ataacttgaa cgaatcgacc cgaatttgat cgctagctct tttttgttat
     1861 caggagccat tattataagt ttatatgttg catagcccgt accggatgcc cttttcttgg
     1921 ttaaagcgcg attccacaaa cctggaacag tggcataaac atgatcggaa tccgatttgg
     1981 atctgtcaga tggataaagt aggtagttat cgtaaaatat ccagttgccg ctaaggttaa
     2041 taacaggaga gcggtctctg tcccagagtg ccagatcaag tatgccgttg cgaatagccg
     2101 gctgttcggt tcgggaagag ggggtacaac ccactattgt aatcaataga atagcggcca
     2161 tcaaaagacg cggcataaaa cctccacaga caatattcgc aaatatacaa tcaagataca
     2221 gaaccactaa aaaaaatcaa acaaagtgag ggtcaatcat gtagatcttt ttagcattag
     2281 tatctttttt tctgtttttg ccatctggtc gcgcagggtc gctgcttgtt caaattcaag
     2341 tttatctgct gcttcgagca tgtcagactt cagttccgta caaagttctt caagttcttt
     2401 tatgctgttc gatctataac ggtttttaaa ttccgatact acagtctcct ccatattaac
     2461 agtgctgtac tctctttcta ttatgtcgta aatgtccttg gatattgttt taggtgatat
     2521 attgttgctg ctattatatt ccgtctggat gactcgacgc ctttcagtct cgtccattgc
     2581 ttttttcatc gagtcggtta ttttgtcggc gtaaaaaatt acgtatccat ttatattccg
     2641 agcggctcta cccgctgttt gtataaggga tcgcgcagac cgtaaaaaac cctctttgtc
     2701 tgcgtcgagg atagcaacaa gtgagacttc cggtatatca agcccctccc gaagcaggtt
     2761 gattcctatc agaacgtcaa attctccttt tctaagatcg cgaattatct cgacacgctc
     2821 aatagtttct atctctgaat ggagatatcg gcatgcgatt ccggagtctt gcagataagt
     2881 tgtaagctct tcagacatct tctttgtcaa ggtagtgact agtacccgct cctttttttc
     2941 tactcttttg tttatctcgc ctataaggtc atcaacctga tttgtggcag gtcttacttc
     3001 aattattggg tctacaaggc ctgttgggcg gatcacctgt tcaccaatta ttgcactttt
     3061 ttccagttca tagtcgctgg gggtcgctga aataaaaaga gcctgatcaa tgatattttc
     3121 aaattcctca aaatagagag gtctgttgtc aagggcacat ggcagacgaa agccgtagtc
     3181 tacaaggttc tgtttccggg atctgtcacc atgatacatt cctcgaactt ggggcaaagt
     3241 gacgtgtgac tcgtcaacaa acatcatgta atcgccttta aaataatcta acagacaatc
     3301 ggggcgttct ccaggttgtc ttcttgaaat aatcctggaa tagttctcaa tacctgaaca
     3361 gtatcccatc tcggtcatca tttcaatgtc ataacgggtt ctgctttcta gccgttgggc
     3421 ctcaacaagt ttgccctttg actgaaaaaa tgctaatt
//
LOCUS       DAOTGZ010000270         3369 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_3064
            NODE_33396_length_3369_cov_4.312613, whole genome shotgun sequence.
ACCESSION   DAOTGZ010000270 DAOTGZ010000000
VERSION     DAOTGZ010000270.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868088
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 3369)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3369)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:22:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,785
            CDSs (total)                      :: 2,751
            Genes (coding)                    :: 2,732
            CDSs (with protein)               :: 2,732
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 12 of 19
            Pseudo Genes (internal stop)      :: 5 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3369
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_33396_length_3369_cov_4.312613"
                     /isolate="AMR_MDS_3064"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..902)
                     /locus_tag="P5123_12710"
     CDS             complement(<1..902)
                     /locus_tag="P5123_12710"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRX17170.1"
                     /translation="MIIALYKKDIRSDTTDNNISTSRIRVFTAPGIFKQAVVAPLLVA
                     TMISFADLHAQQQPEDSPARQEQDDLMSLLEESPKNSFDLKGFAEISSDTGLSDNGYG
                     EQSFYNIGARTKFRPAYSSQKFKAYADIDFFAKSYSHVKEYSFETSEFYIEGENSLLW
                     KAGKQRFNWGVADIFSIANPMDQIDLRESFARNNDEKFSGVYALRLTYIKGDYAIEAA
                     ARPVTEPLMRPAGFYAIHVDSQDTPAGTVTPVYNDTAGERQIKNGAFALRGGGTTSGL
                     DWHLFVYSGPQPRSAYSSTLERTDT"
     gene            complement(899..1651)
                     /locus_tag="P5123_12715"
     CDS             complement(899..1651)
                     /locus_tag="P5123_12715"
                     /inference="COORDINATES: protein motif:HMM:NF028441.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_process: GO:0042953 - lipoprotein transport [Evidence
                     IEA];
                     GO_process: GO:0044874 - lipoprotein localization to outer
                     membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane lipoprotein-sorting protein"
                     /protein_id="HRX17171.1"
                     /translation="MKTANKFFLSIAVFATLTTLPLSSQESAEAIMEKNDEIPSGQSF
                     KREMTLLVIKQGVTEKKEFTGFSKKQGRSRRSRMQFTHPTEMGFLVWDNPGEESQQWI
                     KLSGGRVRRIASTDKGKPWMNSHFYNDDVSEKYIEDYKYELIGEESVNGISCYKIRSV
                     KKGSGATYSKTEVYIGKDDYLTYRVDYFEGGRHVKTLTLTRYENIQNIPTARMLTMER
                     ADGKGKSVLYIKSIRYNISVDNRELTREALAK"
     gene            complement(1654..>3369)
                     /locus_tag="P5123_12720"
     CDS             complement(1654..>3369)
                     /locus_tag="P5123_12720"
                     /inference="COORDINATES: protein motif:HMM:NF015155.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MMPL family transporter"
                     /protein_id="HRX17172.1"
                     /translation="FRMFRGVILPTLSVILAIIWTMGLMGHIGIPITMVVNILPTILV
                     AVGSSYSIHLFNQYLHDRNEIHGNKIRQNLIRSMTRISITVLLAALTTFVGFSTLSIN
                     QVVSLKHFGIFSAIGTLFACAISFMLIPSVLSLFPGTESNKTSGIQNAFLRCAIEKGG
                     RFATNHAATVITVSLVIAIGGIIGLTNINIESSPITQFHDDSYVVTSDSHVSRKLDGT
                     VTFNLMIDTGKDGGVKDPEFLKNIDETAKWIVSQKNRDKYNLLASYSFADVIKRMNKA
                     MNAEDDIFYKLPDKQSTIEDYIMLYSGEDRNSSGIPDAMERFVDPHYRVANIMIKTGT
                     YNGRTYSTKVLLESVDAINRYMKNHGYFNGMRYTIAGQAINYAVLNRLVAWGQVVTIS
                     LTLIIVFGIIFFLFKDIRAALISLIPISFSIIVVYGTMGFLDIPLDLPKSIIAAITIG
                     IGIDDTIHMLKTIRTQMTEGMPLRNAILASYGEAGMAIVYTSIALIFGFSVLMFSQFK
                     VIFYFGWLVAMNMTATTIAALFILPAAAYLFNVNFGLTKKEKSASDTIIQATILTKNK
                     AIQRG"
BASE COUNT          934 a          694 c          710 g         1031 t
ORIGIN      
        1 gtgtccgttc tttcgagcgt actggagtag gccgaacggg gctgcgggcc ggaatagaca
       61 aagagatgcc agtcaagccc tgacgttgta ccgccgcctc taagagcgaa ggcgccattt
      121 ttgatttgtc tttcgcccgc agtatcgttg tatacgggtg tgacagttcc agctggagtg
      181 tcttgtgaat cgacatgtat ggcgtaaaag ccagctggtc gcattagtgg ttctgttacg
      241 ggacgtgctg ctgcttctat tgcataatct cctttaatgt aggttaatct taaagcatat
      301 actccactga atttttcatc gttattgcgg gcaaacgatt ctcgcaggtc aatttgatcc
      361 atggggttag ctatcgaaaa aatatcagcc actccccaat tgaaacgctg tttgcctgct
      421 ttccagagca ggctgttctc accttcgata taaaactctg atgtttcaaa cgagtactct
      481 tttacatgtg aatatgattt agcgaaaaaa tcaatgtccg catacgcctt gaatttttgt
      541 gaagagtaag ccggacgaaa tttggttctt gcccctatgt tgtagaaaga ttgttctcca
      601 tacccattat cagacaaacc cgtgtctgac gatatctcgg caaaaccctt caagtcaaac
      661 gagtttttgg ggctctcttc aagaagagac attagatcat cttgctcctg ccgagccgga
      721 ctgtcttcag gctgttgctg ggcatgtaaa tctgcaaagg aaatcatggt tgccactaaa
      781 agtggtgcga ctactgcctg tttaaaaata ccaggtgcag taaaaaccct gattctactt
      841 gtgctgatgt tattgtcagt tgtgtcagat ctgatatctt ttttataaag tgctattatc
      901 attttgccag tgcctcacgt gttaactctc tattgtctac ggagatattg taacgaatac
      961 tttttatgta aagaacgctc ttgcccttgc cgtcggctcg ctccattgtg agcattcgcg
     1021 cggttggaat attttgaatg ttctcataac gtgtcagggt taaggtcttc acatgtcttc
     1081 caccttcgaa atagtcgaca cggtatgtca gataatcatc tttaccgata taaacttcgg
     1141 tttttgaata tgtcgctcca cttccctttt tgacagatct tattttataa catgagattc
     1201 cgttaacaga ctcttcacca attaattcgt acttatagtc ttcgatatat ttttccgata
     1261 catcatcatt ataaaaatgg gagttcatcc atggcttgcc cttatctgtg gaagcgattc
     1321 ggcgaacacg ccctcccgaa agtttgatcc attgctggct ctcttcgcca ggattgtccc
     1381 agaccagaaa acccatttct gtaggatgtg tgaactgcat tcggctgcga cgagatcttc
     1441 cctgcttttt cgaaaagcct gtaaactctt tcttctcggt gacaccctgc ttaattacaa
     1501 gcagggtcat ctctcgctta aagctttgac ctgacggtat ctcgtcattt ttttccatta
     1561 tggcttcggc gctctcttgt gaactgagtg gaagtgttgt taaagttgca aagaccgcga
     1621 ttgaaagaaa aaatttgttt gctgttttca tgattaacct ctctgtattg ctttgttttt
     1681 agtaagtatt gttgcctgga taatggtatc ggatgctgat ttctctttct tagtaagacc
     1741 aaagtttacg ttaaaaagat atgctgctgc tggcagaata aagagtgcgg cgatagttgt
     1801 ggccgtcata ttcatcgcga caagccagcc aaaatagaag atgactttaa attgtgaaaa
     1861 cataagaaca gaaaacccaa aaataagtgc tattgatgtg tatacaattg ccatgcctgc
     1921 ttctccgtac gaagcaagta ttgcgttgcg aagcggcatg ccttcggtca tctgtgtacg
     1981 aatagttttg agcatatgta ttgtgtcatc tatgccaatt ccaatggtga tagcagcgat
     2041 tattgatttg ggaaggtcaa gcggtatgtc aagaaagccc attgtaccgt acacaacgat
     2101 tatgctgaaa gatattggaa taagcgatat cagagccgcg cgaatatcct tgaaaagaaa
     2161 aaagataatc ccaaaaacaa taatcagggt aagcgatatg gtaacaacct ggccccatgc
     2221 aacaagacgg ttaagcaccg catagttaat tgcctgaccc gcaatggtgt aacgcatacc
     2281 attaaaatag ccatgatttt tcatataacg gttaatagcg tcaacagatt caagcaaaac
     2341 ctttgtcgaa taggtgcgtc cgttatatgt tcctgttttt atcatgatgt tggcaacgcg
     2401 atagtgaggg tcaacaaacc gttccattgc gtcaggtatt ccacttgagt tacggtcctc
     2461 tccgctatag agcatgatat aatcttcaat tgtactctgt ttgtcgggaa gtttgtaaaa
     2521 aatgtcatcc tcggcgttca tagccttgtt cattcttttg ataacatcgg caaacgagta
     2581 tgaggcaaga agattgtact tgtctctgtt tttctggcta acaatccatt ttgctgtctc
     2641 atcgatattc tttaaaaact ctggatcctt gactccgcca tctttacctg tatcgatcat
     2701 caaattaaat gttaccgtac cgtcaagttt gcggctgaca tgtgaatcgg aagtaacgac
     2761 ataagagtca tcgtggaatt gtgtaattgg ggatgactcg atattaatat tagtaagccc
     2821 tataatgcca cctatggcta tcacaagact aacagtaatc actgttgctg cgtgatttgt
     2881 agcgaacctt ccaccttttt caattgcaca acgtagaaaa gcgttttgta taccgctggt
     2941 tttgttagac tcagttcctg gaaaaagaga caagaccgac ggaataagca taaacgagat
     3001 agcacaggca aagagtgtgc caattgcaga aaatatgccg aagtgtttta aagacacaac
     3061 ctgattaatt gatagtgtcg aaaaaccgac aaaggtggtc aacgccgcta aaagaaccgt
     3121 aattgagatt cgtgtcattg aacggataag attttgtctt attttgttgc catgaatttc
     3181 attgcggtcg tgtaaatatt gattaaaaag atgtatagaa taagagctgc caacagcaac
     3241 aagtatagta ggtaaaatat ttactaccat ggtaattggt attccgatat gacccattaa
     3301 tcccatggtc catattattg ccaatattac cgacaatgta ggaagtataa ctcctcggaa
     3361 catcctgaa
//
LOCUS       DAOTGZ010000271         3367 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_3064
            NODE_33424_length_3367_cov_3.770531, whole genome shotgun sequence.
ACCESSION   DAOTGZ010000271 DAOTGZ010000000
VERSION     DAOTGZ010000271.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868088
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 3367)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3367)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:22:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,785
            CDSs (total)                      :: 2,751
            Genes (coding)                    :: 2,732
            CDSs (with protein)               :: 2,732
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 12 of 19
            Pseudo Genes (internal stop)      :: 5 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3367
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_33424_length_3367_cov_3.770531"
                     /isolate="AMR_MDS_3064"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1048
                     /locus_tag="P5123_12725"
     CDS             <1..1048
                     /locus_tag="P5123_12725"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRX17173.1"
                     /translation="STDSISMQGDYNSLLALDKGDDSNYYQSTHLQDIEVYVDSKHLG
                     MLNYNESVNLTLLDPDKIDRLYIKNNNGNIEEINYSNSELKNGKILNGIRTKILELKS
                     NSVCFNGPGKYTIGTNNSLENSVSFRLVDVGTISIDNDCIVTISGFDESLTPKSYNIG
                     YYTNLETNVPRCWRKRIPTIEGDDHELKFKSNTDKTKRLRSRVFKTNLKEIIVSPEDG
                     YEWKYFVIDSYDKDNKIYYNANHGKAYDTKSNYKIEFDPKKYYMFAIRPINANSVDDV
                     TKNYITFEETYQRAHLYPGVIKKTDDISPGLYQFKLDKTKIDGKKIIVHVDYRNEFGK
                     VANESEFDFINYSE"
     gene            complement(1227..2222)
                     /locus_tag="P5123_12730"
     CDS             complement(1227..2222)
                     /locus_tag="P5123_12730"
                     /inference="COORDINATES: protein motif:HMM:NF016852.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LicD family protein"
                     /protein_id="HRX17174.1"
                     /translation="MQTDKKKQVDIVNQAIDTIKYGQTKISAPWWTLVHNIKTPDNYI
                     TIRDLWINYGTKVQNSASVMRAISRAAVICGYHEDSRFMLKKSINRSFNAKRKKKFRK
                     KIRYTVKKIINPFYSFSTKASAALKDLKAACDSIDVRMFLISGTLLGYLRDNKIIGWD
                     KDIDVGFFTEEMSPDKLKAFFADHPDFKVAPVDITSDRIRINHKSGVAIDVFPHYMQD
                     GRRWHNGTATRWWNTDFNLKTISFLKTDFYVPEDPDLYLTENYANWQVPDPNFDARID
                     APNAQVTDQDCLETLFYFGLLDSINKNKPVMIRRYCDILTELGEGAWVDNIKKNY"
     gene            complement(2224..>3367)
                     /locus_tag="P5123_12735"
     CDS             complement(2224..>3367)
                     /locus_tag="P5123_12735"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRX17175.1"
                     /translation="LDNYPQESFFYIIRSEYLMFLDDHDSANKMIQDSRFLYPGNAHC
                     AAISVILCYQTTKDINDADKYALDVLRQFPLNSKVLWSVCKDCTTETQFNEIIKIWSN
                     ALDIKKNKEKLYVKTVAPIVNAATRVCLFKKGIELSLKASLLIVQKNARFQKKPKNLG
                     GKNTFTALEDIYQVFEKCGIPFFFASGTALGIVREGRVLDHDGDIDVGVFENHWSYDK
                     ILDAFKKHPRFIVAKPHPKTPKIGLLHRGGADIDIFKYYKEKEYVYHDGVFVRWRNSP
                     FELKKYSVNGLSFNIPEPADKYLTENYGEWRKPDPSFDAFLYAPNTEVLWQEYRDLHA
                     IRTMYNSLCYNKINRAKRVAKDNINLLAMTSEGKMLAEILLKKELI"
BASE COUNT         1074 a          570 c          609 g         1114 t
ORIGIN      
        1 ttcaactgac agcatttcca tgcaaggtga ttataattca ttattagcct tagacaaagg
       61 cgacgattca aattactatc agtctacgca tcttcaggat attgaagtat atgttgattc
      121 aaaacatttg ggtatgttaa attataatga aagtgtaaat ttaacattat tggatccgga
      181 taaaatagac aggttatata taaagaataa taatggaaac atagaagaaa ttaattatag
      241 taatagtgaa ctgaaaaatg gaaaaattct caatggaata cgtaccaaaa tattggaact
      301 aaaaagcaat agtgtttgtt ttaacggtcc aggaaaatat actataggaa caaataattc
      361 acttgagaat tctgtttcat ttagactggt tgatgttggt acaatttcga tagataatga
      421 ttgtattgtt acaattagtg gtttcgatga atctttaaca ccaaaatctt ataatattgg
      481 ttattataca aatcttgaaa cgaatgttcc acgatgttgg agaaaacgaa tacctacaat
      541 agaaggcgat gatcacgagc taaagtttaa gagtaatacg gataaaacaa aacgattacg
      601 gtcacgggtt tttaaaacaa atttaaagga aattatagta tctcctgaag atgggtatga
      661 atggaaatat tttgttatcg atagctatga taaggataat aaaatatatt ataatgcaaa
      721 ccacggtaag gcttatgata caaaaagtaa ttacaaaatt gaatttgatc caaaaaaata
      781 ttatatgttt gcaattcgcc ctataaatgc taacagtgtt gacgatgtga caaagaatta
      841 tattacattt gaagaaactt atcaaagggc acatctttat ccgggtgtaa tcaaaaaaac
      901 tgacgatata tcaccaggct tgtaccagtt caagttagat aagacaaaaa ttgatggtaa
      961 aaaaatcatt gttcatgttg attatagaaa tgaatttggt aaagttgcaa atgagtcgga
     1021 atttgatttt attaattata gtgagtaatc tctgatatgg gctgaaacta taacaaagaa
     1081 taaaagtgtc agtaaatagc cataaatatt ccataacaag atgaaatatt tatggctaaa
     1141 tcacaataat catctaagta atcttttgtg tcaaacgaat gatttcaaaa tgctaattat
     1201 aggtattgat tttattatat gtataactaa taattttttt ttatattatc aacccaggca
     1261 ccttcaccca attctgttaa aatatcacag taacgtctta tcataacagg tttgtttttg
     1321 tttatgctgt caagcagacc aaaatagaaa agtgtttcaa ggcagtcctg gtcggtaacc
     1381 tgcgcgttcg gcgcgtcaat acgggcatca aaattgggat ccggaacctg ccagtttgca
     1441 taattttccg tcagataaag gtcagggtct tccggcacat aaaaatctgt tttcaaaaaa
     1501 ctgatcgttt tcaggttaaa gtcagtattc caccagcgtg ttgccgtacc gttgtgccat
     1561 cttctgccgt cttgcatata atgaggaaaa acatcaatgg caacacctga cttgtggtta
     1621 attcttattc tgtcagatgt aatatccaca ggcgcgactt taaaatcggg atgatcagca
     1681 aaaaaggctt tcagtttgtc cggtgacatc tcttcggtaa aaaaacccac atctatatcc
     1741 ttgtcccagc caattatttt gttatcccta agatacccca gcagggtgcc gcttatcaga
     1801 aacatacgca cgtcaatgga gtcacatgca gctttcaggt cttttagtgc cgcggatgct
     1861 tttgtcgaaa atgaataaaa ggggttaata atttttttta ctgtatatcg tatttttttt
     1921 ctgaatttct tttttctttt ggcattaaag gatcggttta tgcttttttt cagcataaac
     1981 cggctatctt catggtaccc gcaaattact gcggcacgtg atattgcccg cattacagat
     2041 gcgctgttct gtactttcgt cccgtaatta atccaaagat cgcgaatagt gatataattg
     2101 tctggtgttt taatattgtg tacaagagtc caccaggggg cacttatttt tgtctgcccg
     2161 tatttaattg tgtctattgc ctgattcact atatcaactt gttttttctt gtcggtttgc
     2221 attttaaata agctcttttt ttaatagaat ttcagccagc attttgcctt ctgatgtcat
     2281 tgcaagcagg ttaatattat ccttggcaac tcttttggct ctgtttattt tgttataaca
     2341 taaagagtta tacattgttc taattgcgtg aagatctctg tactcctgcc atagaacttc
     2401 tgtattcgga gcatacaaaa atgcgtcaaa agatgggtca ggttttcgcc attctccgta
     2461 gttctctgta aggtacttat ctgcaggttc gggtatgtta aaacttaaac cgttaacaga
     2521 gtattttttt aattcgaaag ggctgtttcg ccatcgtaca aagacgccat catgataaac
     2581 atattctttc tctttgtaat atttaaaaat atcaatatcc gcgccgccgc ggtgtaaaag
     2641 tcctattttc ggtgttttgg ggtgcggttt ggcgacaata aaacggggat gttttttaaa
     2701 cgcgtccagt attttatcat aagaccagtg gttctcaaaa actccaacgt ctatatcacc
     2761 gtcatggtca agaaccctgc cttcacgaac aattccaagc gccgttcccg acgcgaaaaa
     2821 aaacggtata ccgcattttt caaaaacctg atatatatct tcaagtgctg taaaagtatt
     2881 tttcccccca aggttttttg gttttttttg aaaacgcgca tttttctgaa caattaaaag
     2941 tgacgccttt aacgaaagtt cgataccttt tttaaaaaga caaacccggg tcgcggcgtt
     3001 aacaatgggc gcgactgttt ttacataaag cttttcttta ttttttttaa tatcaagtgc
     3061 attactccag attttaatta tttcgttaaa ttgagtttct gtagtgcaat ctttgcaaac
     3121 gctccagaga accttgctgt ttaagggaaa ctgtcttaaa acatcaaggg cgtacttgtc
     3181 tgcatcatta atatctttcg tggtctgata gcaaagaatc accgatatag ccgcgcaatg
     3241 agcattgccg ggatacagaa atcttgagtc ttgtatcatt ttgtttgcgg aatcatgatc
     3301 atcaagaaac attaaatatt cagaacgtat tatgtaaaag aatgactctt ggggataatt
     3361 gtctaac
//
LOCUS       DAOTGZ010000272         3352 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_3064
            NODE_33607_length_3352_cov_5.518047, whole genome shotgun sequence.
ACCESSION   DAOTGZ010000272 DAOTGZ010000000
VERSION     DAOTGZ010000272.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868088
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 3352)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3352)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:22:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,785
            CDSs (total)                      :: 2,751
            Genes (coding)                    :: 2,732
            CDSs (with protein)               :: 2,732
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 12 of 19
            Pseudo Genes (internal stop)      :: 5 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3352
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_33607_length_3352_cov_5.518047"
                     /isolate="AMR_MDS_3064"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1077)
                     /locus_tag="P5123_12740"
     CDS             complement(<1..1077)
                     /locus_tag="P5123_12740"
                     /inference="COORDINATES: protein motif:HMM:NF012551.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endo-1,4-beta-xylanase"
                     /protein_id="HRX17176.1"
                     /translation="MLRHYKNCKCLPKHYCMVRILLQIILITCVTSIFISLFSCDPLK
                     KYDSEIETDITPIKEVFKDDFTVGAAIEPNQLEGAEGALLRYHFSGLTAENAMKFKTI
                     HPKRKVWSFEKADKIVKFARKYDMKVRGHAFVWHHPAEHAHWAFLNMDGSKKSREETL
                     EILKEHITTLMDRYKDDVYCWDVVNEAIDEYEKDGMRKTEWYRRIGPDYVAEAFKIAH
                     KADPKIKLFYNDYENYKPNKRKLIKKFIKGMLDDGVPIHGIGMQLHISNGFPSIQMIE
                     DAINDYRDLGLEIHVTELDISMYAHEYDKIDGTPSEAHLVHQAYRYKQLMELFRKHKD
                     IVTNVTLWGYHNGHTWLTGAPYNRP"
     gene            1385..2098
                     /locus_tag="P5123_12745"
     CDS             1385..2098
                     /locus_tag="P5123_12745"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRX17177.1"
                     /translation="MYTLQKYISDLMREKRIKESVVLERIGYTNLSKGTRRFRAFMDG
                     DMSNSFLINNLHKGIGVDRSEIKKMADKTREEIQQAIEEEVLRRQAKERQNFVPYLYV
                     KTEREIPSPIFAYAIMGFGRLRRILLPENFNYLSELEKSEIIKKIINKWQNAYEGIVP
                     TLGRIRYYILREVYDDIEENRKVYDITGTPALGEEYQDKSIDNGHATLSVKGQSLDNI
                     IKSLHVLEKVQEDPEPFIH"
     gene            2632..3147
                     /locus_tag="P5123_12750"
     CDS             2632..3147
                     /locus_tag="P5123_12750"
                     /inference="COORDINATES: protein motif:HMM:NF023043.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4352 domain-containing protein"
                     /protein_id="HRX17178.1"
                     /translation="MKSKNFKSVIAVLPILILFVLLGLACSDSNEPQIQAKINEKIAT
                     EKFEVTVTSVKSKTKVGTDFIEEVAPEGAVFVIIDFNYKNITDQPINSFSLPDIKIID
                     PNNAAYDEAIGASASYSTQMKLETKIASDLNPGIGQKDAAVFEVSKDIWAQKGWKMII
                     KADKNIEILIP"
BASE COUNT         1086 a          548 c          650 g         1068 t
ORIGIN      
        1 aggacggtta taaggtgccc ctgttaacca ggtatgtccg ttatgatacc cccataatgt
       61 gacgttggta acaatatctt tatgtttgcg gaaaagttcc atcaactgtt tgtatctata
      121 ggcttgatgt acgagatgtg cctctgatgg tgttccatct atcttgtcat attcgtgagc
      181 gtacattgag atatcaagtt cggtaacgtg aatctctagt ccaaggtcac ggtaatcgtt
      241 aatagcgtct tcaatcattt gtatcgaagg aaaaccatta ctgatatgaa gctgcatgcc
      301 aataccatgt attggaactc cgtcatcaag catgccttta ataaacttct taatcagttt
      361 tcgtttgtta ggtttatagt tctcataatc attgtaaaaa agcttaattt tgggatcagc
      421 cttgtgagcg attttgaagg cctctgctac ataatcgggt ccaatacgac gataccattc
      481 agttttacgc ataccgtctt tttcatattc atcgatagct tcatttacaa cgtcccagca
      541 gtagacgtca tccttataac gatccataag tgtagtaata tgctccttaa gaatttcgag
      601 agtctcttcg cgggattttt ttgaaccatc catattcaga aaagcccagt gagcatgttc
      661 ggccgggtgg tgccatacaa aagcgtgtcc gcgaactttc atatcatatt ttctggcaaa
      721 ttttacaatt ttatcagctt tttcaaaaga ccagactttg cgtttagggt gaatggtttt
      781 aaacttcatc gcgttttcgg cggttaatcc gctgaaatga tatctaagca atgctccttc
      841 ggccccttct agctggttgg gctcaatagc cgcaccaact gtaaaatcat ctttaaagac
      901 ttcttttatt ggagtaatat ctgtttcgat ttctgaatca tattttttta ggggatcgca
      961 ggaaaataga gaaataaaga tactggtaac acatgtgatg agtataattt gcaataaaat
     1021 tcgaaccata caataatgtt tgggcaaaca tttgcaattt ttgtaatgtc gcaacataaa
     1081 gacctccggg aaacgggtta tttgattttg taaaacttaa gagtaataaa aggcaagtta
     1141 gatttatttt caaccaacgt tgactggata agggaatttt gtcaacatta attcaaccga
     1201 ttgcacttca tcttagaaat cagcttaatt tggacattta tgatattgat ttagattcat
     1261 ttagtggctt cttcaacata gacattcact tgctaccttt tttacatgct atcttgtatt
     1321 ttgtatttat tgctttgttt gaaaagactt gtacgtcaga aaaatcataa gtggggagga
     1381 ttaaatgtac acattacaaa aatatatcag tgacttgatg agagaaaaaa gaataaaaga
     1441 atcggttgtg cttgaaagaa tcggatatac caaccttagc aaaggaacca gaagatttcg
     1501 ggcttttatg gatggagata tgtcaaactc gtttctgatt aataatctgc acaaaggcat
     1561 tggtgtagac agatctgaaa ttaagaagat ggcagacaaa acacgggaag agatacaaca
     1621 agcaatcgaa gaagaggttc tgcgccgcca ggcaaaggaa agacaaaatt ttgttcccta
     1681 tctttatgtt aaaacagaaa gagaaatacc ttcaccaatt tttgcttacg caataatggg
     1741 ttttggtcgg cttagacgga tacttttacc tgaaaatttt aattatttat cggaacttga
     1801 aaaatctgag attataaaaa agataataaa taagtggcag aacgcatatg agggtatagt
     1861 acctacatta gggagaatcc gttattatat tttgcgggaa gtctatgatg atattgaaga
     1921 aaatcgtaag gtttatgata ttacgggaac tccagccctg ggcgaagaat accaagataa
     1981 aagtatcgat aacggccacg cgactctttc agtaaagggg cagtctctgg acaatatcat
     2041 taaatccctg catgtgcttg aaaaagtaca agaggacccc gaaccattta ttcactaaaa
     2101 tcgtatgtta gaattttttc aaaaaaaatc taattttatc caagatagag gctcagtcgc
     2161 tatcttggat aaaactgctt tggtataatg tttgtatagt tgatttaaaa cgcagcaagt
     2221 ctgttagcct tgattgcatt ttgcaaaaag aacggagttg tttaataaaa tcctaaacag
     2281 gaaaattgat tatgtatgat aaattgttca gtttgttaag tgctatatgt catcaaaatc
     2341 cccaaagatc attcttttgg ggtgacgttc agtttatact ctgcgcgcga tgcactggca
     2401 tctacttagg agtcctattt actttgattt tatgcccact tgttaaagta aatgtctctc
     2461 tcagttttct gtatctgata ctcttctgtg tacttattat caatatgata acatttataa
     2521 caatgtttga taccaatata gttcgactgg tgaccggttc ggctattggt attttatctg
     2581 gttttgtaat tatcaaatca ataaaaaata ttttatcaaa ggagaatcaa aatgaagtca
     2641 aaaaatttta aatcagttat tgcggtttta ccaatactaa tattatttgt attgttaggg
     2701 ttggcctgca gtgatagcaa cgagcctcaa atccaggcca agattaatga aaaaatagct
     2761 acagaaaaat ttgaggtaac ggttacatct gttaaatcaa agactaaagt gggaacagat
     2821 tttattgagg aggtagcacc ggaaggagct gtttttgtaa ttattgattt taattacaaa
     2881 aacataactg atcagccaat caactctttt agtttaccag acataaagat tattgaccct
     2941 aataacgcgg catatgatga agcaatcggg gcatcagcaa gttattcgac tcaaatgaag
     3001 ctcgaaacta aaatagcgag tgatcttaat cctggtattg gtcaaaaaga tgctgcagtt
     3061 tttgaggtat caaaggatat ctgggcacaa aaaggctgga agatgataat taaagctgat
     3121 aagaacattg agatccttat tccataaata atacttatat catcgggccc cggtaatttt
     3181 ttagccgggc ctggcgatat aaggatgtat tgttagcgtt aagagttgaa taaaaataga
     3241 atttggattg taacatgatt acatttaaag atgataacaa gttaatagaa ttatcgatca
     3301 acgattatca gtatccagaa attacagagg gatattatga ctgtgactgg ct
//
LOCUS       DAOTGZ010000273         3314 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_3064
            NODE_34067_length_3314_cov_4.973305, whole genome shotgun sequence.
ACCESSION   DAOTGZ010000273 DAOTGZ010000000
VERSION     DAOTGZ010000273.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868088
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 3314)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3314)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:22:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,785
            CDSs (total)                      :: 2,751
            Genes (coding)                    :: 2,732
            CDSs (with protein)               :: 2,732
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 12 of 19
            Pseudo Genes (internal stop)      :: 5 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3314
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_34067_length_3314_cov_4.973305"
                     /isolate="AMR_MDS_3064"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(66..377)
                     /locus_tag="P5123_12755"
     CDS             complement(66..377)
                     /locus_tag="P5123_12755"
                     /inference="COORDINATES: protein motif:HMM:NF016876.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008657 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) inhibitor activity
                     [Evidence IEA];
                     GO_function: GO:0042803 - protein homodimerization
                     activity [Evidence IEA];
                     GO_process: GO:0009432 - SOS response [Evidence IEA];
                     GO_process: GO:0030541 - plasmid partitioning [Evidence
                     IEA];
                     GO_process: GO:0051290 - protein heterotetramerization
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HRX17179.1"
                     /translation="MYEITYLPIAEQDIAEIIIYISNQLKAPKAAIELLDAFDLSIML
                     LQEFPYTHKIYRLTKPLEEEYRLLPIKNYAVFYVVREQHKIVEIHRVVYAKMDLSQIL
                     K"
     gene            complement(370..657)
                     /locus_tag="P5123_12760"
     CDS             complement(370..657)
                     /locus_tag="P5123_12760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012033535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0110001 - toxin-antitoxin complex
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system Phd/YefM family
                     antitoxin"
                     /protein_id="HRX17180.1"
                     /translation="MLQIRPVSDLRNNFADISKIVHETARPVFLTKNGYGDMVVMSFE
                     AYEKLQFESEIYFKLKEAEMQARLTNQRFSHKEVFNRLRQTLMEKADNENV"
     gene            complement(742..1314)
                     /locus_tag="P5123_12765"
     CDS             complement(742..1314)
                     /locus_tag="P5123_12765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015053762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3795 domain-containing protein"
                     /protein_id="HRX17181.1"
                     /translation="MKEDSQIKIKYPEIGVCGLSCRLCPNYHMNTKSRCLGCKSKDRI
                     AVGCPFITCAVKKRRIEFCWDCKDKKTCEKWKKHREKGKEHDSFKCYQTLEEDISFLE
                     ENGAVEFEKLQKVKERILVKMLEDFNEGRSKSYYCIAASVLKTEELKEALRKAENESD
                     GLDITGKSKVLHKALDEIAGSKKYCLKLRK"
     gene            complement(1342..1638)
                     /locus_tag="P5123_12770"
     CDS             complement(1342..1638)
                     /locus_tag="P5123_12770"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRX17182.1"
                     /translation="MQLLDNYNDQRRKSFFNLLEPQGIKGVIVQIADEIKPDMSVKVK
                     SATAVSLFQAIAKKRNITLKLNKESVKNSRSIYTSLFVKTAAQQWVINIAKQLV"
     gene            complement(1974..>3314)
                     /locus_tag="P5123_12775"
     CDS             complement(1974..>3314)
                     /locus_tag="P5123_12775"
                     /inference="COORDINATES: protein motif:HMM:NF027787.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5050 domain-containing protein"
                     /protein_id="HRX17183.1"
                     /translation="RFLGLESVWNSSEGLKIAKTGVNWEYHKYQEAGKNNIVYSATIA
                     SFKINVNGKQIDNRNEKYPLLLFRNITYFPLTWRFAVDECDWNYSFDQQNGLVINSTV
                     GDITATQVTIPIVTRENGSKGPFTKAGDYLYYEGQQGKIYQTPASDPTDSREVYQLPP
                     SFFDNHYPALASLTTDNGKALLKYHTGGATMGSDHIVWLKEDGSAEEIDSGYSALKLY
                     DEYTLKVDHWAPPIANNLLIKKQGEKQYIEVGDPKCYFGRFFLDYGHGLSSKPSQDLY
                     LIDDKIYVLGYYQNENLPTTTGIYKVDINTGATVRICTEEADGFKIVDGMIYFTDRSQ
                     YLYRVPVSGGKAELLIDKAVSHYEALKGHVYYALADSGQLFTIGSEKSINPGGKLNKM
                     EIQNDYLVVIFDKDSKSPYKMMIIDEDGQELYKTIENVLLVQIENGKVIFVKDN"
BASE COUNT          919 a          731 c          575 g         1089 t
ORIGIN      
        1 gctatctgtg aagaagatat ggggcgctgc tcaaaacttg tagccgacga caaatgcccc
       61 ggccttcatt ttaaaatttg gcttaaatcc atctttgcat aaaccactcg atggatttct
      121 acaatcttat gctgctctcg tacaacataa aataccgcat aattctttat cggcaacaaa
      181 cggtattctt cttctagcgg ttttgtcaac ctgtatattt tgtgggtgta aggaaactcc
      241 tgtaatagca taatcgaaag gtcaaacgcg tccagcaact ctatcgcagc tttcggtgct
      301 ttcaattgat tagaaatgta tataataatt tcggcaatat cctgttcagc aatcggcaga
      361 taggttatct catacatttt cgttatcggc cttttccatt agggtttgcc tcaaacggtt
      421 gaagacttct ttatgggaga atcgctggtt ggttagcctg gcttgcatct cagcctcctt
      481 gagtttaaaa tatatttcac tttcaaactg aagtttttca taggcttcaa aactcataac
      541 aaccatatcg ccatacccgt ttttggttaa aaataccggt cgcgcagttt cgtgaacgat
      601 tttggaaatg tctgcaaaat tatttcgtaa atcggatacc ggtctaattt gtagcataaa
      661 gataacacct ctcactttaa atttagtgta attttatcat aattgcgcta aactacgcaa
      721 gatatatatg ggcataaatt attatttcct caatttcaga cagtacttct tgctcccagc
      781 aatttcatcc aacgctttat gtaatacctt tgatttgcct gtaatatcaa gaccgtcaga
      841 ttcattttct gcttttcgca aagcctcttt caattcttcc gtttttagaa ctgaagcagc
      901 tatgcaataa tagctttttg aacgtccttc gttaaaatcc tccagcattt ttaccagaat
      961 ccgttcttta accttctgca atttttcaaa ttccacagct ccgttttctt caagaaagga
     1021 aatgtcttct tcaagggttt gataacattt gaaagagtca tgttctttgc ctttttctct
     1081 atgttttttc cacttttcac aagttttctt atccttacaa tcccagcaaa actcgattct
     1141 ccttttcttt actgcgcagg tgatgaacgg gcagccgaca gcgattctgt ccttgctttt
     1201 acatcctaga catcggctct ttgtattcat atgataattc gggcaaagcc tgcaggaaag
     1261 tccacacaca ccgatttcag gatatttgat cttaatttga ctatcttcct tcataacact
     1321 cccccctatc ggttttgtct ttcatactaa ttgtttagca atgttaatca cccattgctg
     1381 tgccgcagtt ttaacaaaaa gtgaggtata aatggaacga ctatttttta cgctctcttt
     1441 attcagcttt agtgttatat tacgcttttt cgcaatagcc tggaacaaac taacggcagt
     1501 tgcagacttt acttttaccg acatatccgg ttttatttca tccgctattt gtactatcac
     1561 acccttgata ccttgtggct ctaaaagatt gaaaaagctt tttcgccgtt gatcgttata
     1621 attatcgagt aattgcaatt gatattacca ggaatacaac cggtatggct acaattgtag
     1681 ccagcataac gatgattact cgcacttttt catcattttt aaagattaat gttaagactt
     1741 tataacttaa aataccaact aaaccgccga aggtgttcaa gatgacatcg tcaatatcgc
     1801 ttactccaag tccaaatata tattggatta tttccacgag caagctaatt aaaataacta
     1861 gcctaggaaa ctttggtgcc atccgctatg tggatacatt ggcgtgggta gcccggctcc
     1921 cagccatgga gttatctgga gagataatcc ggggcaaata atataaaaaa cgtttaatta
     1981 tctttgacaa aaatcacttt gccgttttcg atttgcacta acagcacatt ttcaatagtt
     2041 ttatacagct cttggccgtc ctcatcgata atcatcattt tataaggaga cttactgtct
     2101 ttatcaaaaa tcaccaccag ataatcattt tggatctcca ttttattcag ttttcctcca
     2161 ggattaattg acttctcgct gccaatagta aatagttgac cgctatctgc cagcgcataa
     2221 tatacatgtc ccttaagagc ttcataatgg cttaccgcct tatcgattaa aagttctgcc
     2281 ttgccgccgc tcaccggaac ccggtataga tactgactac ggtcggtaaa gtatatcatc
     2341 ccatctacaa ttttaaagcc gtctgcctct tctgtacata tcctaaccgt tgcaccagtg
     2401 tttatatcga ctttgtagat accggttgta gttggtaagt tctcgttttg atagtaacca
     2461 agcacataaa tcttatcgtc aatcaaatat aaatcctgac tgggtttaga tgaaagacca
     2521 tggccataat ccaggaaaaa ccttccaaag tagcatttcg ggtcacccac ttcgatatat
     2581 tgcttttcac cctgtttttt gatcagcaga ttgttggcaa taggcggagc ccaatgatcg
     2641 acctttaagg tgtattcatc atataacttt aaggcagaat aaccgctgtc aatttcctct
     2701 gccgaaccat cctctttcag ccagacaata tggtcacttc ccatggtagc accaccagta
     2761 tgatatttta aaagagcctt gccgttgtca gttgtcagtg aagctaacgc cgggtagtgg
     2821 ttgtcgaaaa aactgggtgg caattgataa acttctctac tgtcagttgg gtcagatgcc
     2881 ggtgtctgat aaattttacc ctgctgtccc tcataataaa gatagtcccc cgctttagta
     2941 aaaggacctt tgctgccatt ttcacgagtg acgataggaa tcgtcacctg ggtagcagta
     3001 atatcaccaa cagttgaatt aatcaccaag ccgttttgct gatcaaatga ataattccaa
     3061 tcacattcgt cgaccgcaaa cctccaagtc aggggaaaat aagtaatatt gcgaaatagc
     3121 agcaagggat atttttcatt gcggttatcg atctgtttac cgttgacatt gattttgaaa
     3181 gaagcaatgg tcgctgaata aacaatatta tttttgccgg cttcctgata tttatggtat
     3241 tcccagttga cgccggtttt ggctatctta agtccctcgc tgctgttcca tacactttcc
     3301 aagcccagga agcg
//
LOCUS       DAOTGZ010000274         3279 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_3064
            NODE_34523_length_3279_cov_5.730769, whole genome shotgun sequence.
ACCESSION   DAOTGZ010000274 DAOTGZ010000000
VERSION     DAOTGZ010000274.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868088
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 3279)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3279)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:22:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,785
            CDSs (total)                      :: 2,751
            Genes (coding)                    :: 2,732
            CDSs (with protein)               :: 2,732
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 12 of 19
            Pseudo Genes (internal stop)      :: 5 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3279
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_34523_length_3279_cov_5.730769"
                     /isolate="AMR_MDS_3064"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1858)
                     /locus_tag="P5123_12780"
     CDS             complement(<1..1858)
                     /locus_tag="P5123_12780"
                     /inference="COORDINATES: protein motif:HMM:NF013106.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglycosylase domain-containing protein"
                     /protein_id="HRX17184.1"
                     /translation="MFQKKYNGTSFRVKRPINDLNSKPVLKKKYAGLNLRAIKDLFSG
                     TPRKSRLSGEKPNFFFMFSSIFSFFKKESSKKVVTEKPSIIKTVKREPSKEKKAMVMT
                     SGGDNHESANNDEKKQDKRVITRSSVSRRPLFRLPSIRFSLPRFSFFKFPSFGGGSSR
                     ASSAGGGSFGTGGSTLYLVWSFIRRYYIAIIACFSIMIIVGSIFFVNYLKEEYKNVEA
                     LTGFQPNVVTRIYDRNGLLISELFTQKRDLVNYEAIPQNLIDAFIALEDNDFYQHYGV
                     NPKGIIRAFFINIASGRVRQGGSTITQQLAKILLTDRERKLTRKIKEACIAIMIEFTY
                     DKRKILEIYLNQIFLGHGAYGVEAASQLYFSKSVSELNLAECALLATLPSAPNRYSPI
                     RHTKRSMGLHKVALAKMVDLGFITVQQAEEAYLSFWPEYQLFINELSPSYNTWSLRVD
                     EAPWVTEYVRRYIVDKFGEDAVYKEGLEIYTTFDLKKQLLAQQLMQERLREQTETSSK
                     LLFTDDDFFTENFASELSFLSLLFDANPISRRGSFENKKFNDHMGQEITDCLEIINYF
                     SGHAGLAGFIDFYRETSGTDRFLLPVEGALVSINQQTGEIEAMVGGSRFSSMN"
     gene            2144..>3279
                     /locus_tag="P5123_12785"
     CDS             2144..>3279
                     /locus_tag="P5123_12785"
                     /inference="COORDINATES: protein motif:HMM:NF012707.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter substrate-binding domain-containing
                     protein"
                     /protein_id="HRX17185.1"
                     /translation="MVTEKREKSKSRFLFPNIIRKFFFLLIFLLFAFTSILLSAITDN
                     ETDYNAAEAVLSAEELIWLEKHPVIKIAPDPEFRPIEFFDSNGTYSGMASDYAMLIGK
                     KLGISFTVVKCKDWNEVMEKVRNQEVDILNAVIRTPDREKYLNFSYPYLRIPSVIIVK
                     DSDSKYRTLDSLKNKTAVMVQNYGYTELIIKQNPLLKIITASNLMEALKTVSLGEADA
                     FIGDLATASYYIRTENLGNLRVSAEIEPPNISGFAVRSDWPELISILDKGISLLSNEE
                     KLIIHRKWINVTPPHAISNDQIRNAVIIVSAIFLFIFAATMTWIHTLRKMVHLKTEQL
                     QNELQEREKMERISAESESRLQAFLSTIPDPVWLKDKDGKFLFC"
BASE COUNT         1044 a          645 c          597 g          993 t
ORIGIN      
        1 ggttcatgga cgaaaagcga cttccaccaa ccattgcctc tatctcgccg gtttgttgat
       61 ttatcgaaac gagagcacct tcaacgggca acaaaaacct gtcggtacct gaagtttcac
      121 ggtaaaagtc tataaatcct gctaatccgg catgtccgct aaaataatta attatttcaa
      181 gacaatcagt aatctcctgc cccatatgat cgttaaattt tttgttctca aaagatccac
      241 gcctgcttat cgggttagca tcaaatagaa gcgataagaa tgaaagctca cttgcaaaat
      301 tttcagtaaa gaaatcatca tcggtaaaca acagctttga tgatgtttca gtctgctcgc
      361 gaagcctctc ttgcataagc tgctgagcca aaagttgttt ttttaaatca aaggtcgtat
      421 aaatttcaag accttcttta tatacagcat cttctccaaa tttatcgact atatatcgtc
      481 gaacatactc tgttacccaa ggtgcttcat ctactcgcag agaccaggta ttgtaagaag
      541 gcgatagttc attaataaaa agctgatatt ccggccagaa agagaggtaa gcctcctctg
      601 cctgttgaac agttataaaa ccaagatcga ccattttggc aagggcaacc ttatgtaagc
      661 ccatagaccg cttggtatgt cgaatcggag aataacggtt tggcgccgat ggaagcgttg
      721 ccaaaagcgc gcactccgca agatttaatt ctgaaacact tttggaaaaa tataactgtg
      781 aagcagcctc tacaccgtat gccccatgcc ccagaaaaat ctgattaaga taaatctcca
      841 gaattttgcg tttatcgtag gtaaactcta tcattatcgc gatacaggct tctttgattt
      901 tacgagtaag ctttcgctcg cgatcggtta aaagaatttt agcgagctgc tgggttatgg
      961 tgctacctcc ctggcgaacc cgtccggagg caatatttat aaaaaacgct cgaattatac
     1021 ctttgggatt aacaccataa tgctggtaaa aatcgttatc ctcaagagca ataaatgcat
     1081 cgataaggtt ctgcggaatc gcctcgtaat taacaagatc tcttttttga gtaaatagtt
     1141 ccgatataag tagaccgttg cggtcataaa tacgagtaac cacatttggc tgaaatccgg
     1201 ttagagcttc aacatttttg tactcttcct tgagataatt gacaaaaaat atagagccga
     1261 caattatcat aattgagaaa caggctataa tcgcaatata gtatcttcga ataaaagacc
     1321 agacaagata aagtgtggat ccacctgttc caaaagagcc gcccccagcc gaagaagcac
     1381 gagatgaacc tccaccaaaa gagggaaatt taaaaaatga gaatcgagga aggctaaatc
     1441 ttatcgaagg caatctgaac aagggacgtc gagatacaga tgatcttgtg attactcttt
     1501 tatcctgttt cttttcatca ttattagcag actcatggtt atcacctcca gaggtcatca
     1561 ccattgcttt tttttcttta gagggttccc gtttaacagt tttgattatg gatggctttt
     1621 cagttactac ctttttcgat gattcctttt taaaaaaaga gaatatagaa gaaaacatga
     1681 aaaagaagtt gggtttctca cccgagagcc tggattttcg tggagtaccc gaaaaaagat
     1741 cctttatcgc gcgcaaatta agacccgcgt attttttctt tagtacaggt ttcgagttta
     1801 ggtcattaat aggtctttta acacgaaaag atgtgccgtt atattttttc tgaaacatta
     1861 tttaaaatta tctcttttat tatattttta caatagtacg cttttatata ctaccttaga
     1921 gagtgtcact ctaatttttt ttatttttat tgaaaagaat attttcaaat tgcgcataga
     1981 cgaaaataag atcgtatcaa atcgtagttc agctataaat cgagttattt ttactagctt
     2041 tgtttctaaa gtcacgtttt gattgattaa taatccaagc actcatagtc tggttactgt
     2101 ttcaataata atattccttt aaaaaacaaa ggattgaatc agtatggtaa cagaaaaacg
     2161 ggaaaaaagt aaatcaagat ttttatttcc taatataatc cggaaatttt tctttttgct
     2221 aattttcctg ttatttgcct ttacctcaat cttgcttagt gcaattaccg ataacgagac
     2281 agattataat gctgcggaag ctgttttatc agcggaagag cttatatggc tggaaaaaca
     2341 tccggttatt aaaatcgctc ccgatcccga attccgccca atagaatttt ttgactcaaa
     2401 tggtacatat tcgggtatgg cttccgatta cgccatgcta attggaaaaa aactgggaat
     2461 cagtttcact gtagttaagt gcaaagactg gaatgaggta atggaaaaag tccgaaacca
     2521 ggaagttgac atattaaatg ctgttattag aacccccgat cgtgaaaaat atcttaactt
     2581 ctcttatccc tatcttagaa ttccatctgt aattatagta aaagacagtg attcgaagta
     2641 ccgcacactt gattcgttaa aaaacaagac ggctgttatg gtccaaaatt atggatatac
     2701 agagcttatc atcaaacaaa accctcttct taaaataatt acagcttcta accttatgga
     2761 agctcttaaa accgtttctc ttggggaagc tgatgccttt atcggagatt tggctacagc
     2821 aagctactat atacgtaccg agaaccttgg caatttacga gtatctgctg aaatcgaacc
     2881 tcccaatatt tccggttttg ctgtacgctc tgactggccc gagctaatta gcatacttga
     2941 caaaggcata agcctgttat caaatgaaga gaaattgatc attcaccgca aatggatcaa
     3001 tgtaacacca ccccatgcaa tttccaatga tcaaattcgc aatgcggtta ttatagttag
     3061 tgccatcttt ctatttattt tcgctgccac tatgacatgg atacacactc tgcgcaaaat
     3121 ggttcattta aaaacagaac agttgcaaaa tgagctacaa gaaagagaaa aaatggaacg
     3181 tatttctgcc gaaagcgagt cacgtctgca ggccttttta agcacaattc ctgatcccgt
     3241 gtggcttaaa gataaagatg gtaaatttct tttttgcaa
//
LOCUS       DAOTGZ010000275         3278 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_3064
            NODE_34550_length_3278_cov_3.681043, whole genome shotgun sequence.
ACCESSION   DAOTGZ010000275 DAOTGZ010000000
VERSION     DAOTGZ010000275.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868088
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 3278)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3278)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:22:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,785
            CDSs (total)                      :: 2,751
            Genes (coding)                    :: 2,732
            CDSs (with protein)               :: 2,732
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 12 of 19
            Pseudo Genes (internal stop)      :: 5 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3278
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_34550_length_3278_cov_3.681043"
                     /isolate="AMR_MDS_3064"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..640)
                     /locus_tag="P5123_12790"
     CDS             complement(<1..640)
                     /locus_tag="P5123_12790"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRX17186.1"
                     /translation="MKIIICSDVNKTEDLFSSLTKNKNHDITFASALDYRKVLKDAPR
                     RTLFYLDLSKLDDSAISKEVNYLIKQEGVFTALIDPKGKIADIASLFHKGLVDYIGKA
                     LYTGNSGIDARRINTVEEYIKKGCPEAEQSAILYDIDEKMIVRKDWSNVKPGTEYTFL
                     FLHLFFDNYYEIEKTYGRAHVLKMVNKFKDYLSHVVSPYGGRVWMWMEASGLV"
     gene            complement(641..1564)
                     /locus_tag="P5123_12795"
     CDS             complement(641..1564)
                     /locus_tag="P5123_12795"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRX17187.1"
                     /translation="MKVKAALVNICIKALAESMVSDMMLSVVKEVMPNYDIYKQTGFP
                     QNMAIPNRDIARQIIMDVNENHFFPPFILELIKLHRMGYRGRPCSISYMDQILKGMRA
                     NGLIYDPKNHIFVEDPNVQRTRNWNVFREGQEAVITLLKIDIVDNSVLVKENPKPKVD
                     AAYEIFRNQVFENIERVNGRVWNWEGDGGIGAFYFGDKKVSALLSAINILHEILYFNL
                     FKNELKNPLQVRIALHDGRIIYSDVEKDLFNNETVKQVINIESKHTRPNTITISDMMT
                     TSYDAMIKDQLESFRGKDNKRYYRYSVQWEE"
     gene            complement(1684..3126)
                     /gene="purF"
                     /locus_tag="P5123_12800"
     CDS             complement(1684..3126)
                     /gene="purF"
                     /locus_tag="P5123_12800"
                     /EC_number="2.4.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015695512.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004044 - amidophosphoribosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidophosphoribosyltransferase"
                     /protein_id="HRX17188.1"
                     /translation="MCGIVGFYGPPSKKAVYDIISGLLSLQHRGQDSVGVVTFDDKFH
                     VKKGLGLVSNVFKKVNLDSLPGSIGMGHVRYTTQGSNEIMNVQPFTVNYPFGLSMVHN
                     GNVINFNYLRKYLYEEHHRLLETSNDLELLLYTLASELELHDLKNLSIDDIFDSVKST
                     QQKVRGAYATITVIANKGLLVFTDPYGIRPLVMGKKDTPDGPCYGFASESTCFDYLDY
                     EVIGDIGAGEIVFIDNDLNVHRRTGFVEKAAHCIFCYIYFSREDSIMRGQLVADLRVK
                     MGRMLAEKMREEGFEPDIVIDVPSSAYFFASGLSEELGIPYRRGFAKNNHIGRSFISP
                     TQSEREKVVRNKLNPIREVVKGKKVAVVDDSIVRGTTSKHIVSLLRKAGADKVYFVSA
                     APPIVSPCIYGIDMATKGELIANCGSIDKINEIIGADATIYQSLEDLQKLCEKVKLPV
                     CDACFSGNYPTGDTREIFAEIEQERLSSRK"
BASE COUNT         1019 a          700 c          519 g         1040 t
ORIGIN      
        1 gaacaagccc cgaagcttcc atccacatcc aaaccctacc cccataagga gacaccacat
       61 gtgaaagata atctttaaat ttatttacca ttttcagaac atgagcacgg ccataagttt
      121 tttctatctc atagtaatta tcaaaaaaca gatgaaggaa tagaaatgta tattcggttc
      181 cgggtttaac attactccag tctttacgca caatcatttt ttcatcaata tcataaagta
      241 ttgcagattg ttccgcttcc ggacatcctt tctttatata ttcctcaaca gtattaattc
      301 tgcgtgcatc aataccgctg tttcccgtat acaatgcttt accaatataa tcgacaagac
      361 ctttatgaaa aagtgaagca atatctgcga ttttaccctt tgggtcaatc aaagctgtaa
      421 aaacaccttc ttgcttaatc agataattta cttcctttga tatagcagaa tcatctaact
      481 tggataaatc caaataaaat agagtccttc gaggggcatc ttttagtact ttgcggtaat
      541 ccaatgctga agcgaaggtt atatcatgat ttttattttt tgttaatgaa gaaaaaagat
      601 cttccgtttt atttacatcg gagcatatta ttattttcat ctactcttcc cattgtacag
      661 aatagcgata gtatctttta ttgtccttac cgcgaaaaga ctcaagctgg tctttaatca
      721 ttgcgtcata cgatgtagtc atcatgtcgc taattgttat tgtattgggt ctagtatgct
      781 tggattctat attgattacc tgcttgactg tctcgttgtt gaaaagatct ttttcgacat
      841 cagaataaat aatcctgccg tcatgcaatg ctatgcgaac ctgaagcgga tttttaagtt
      901 catttttaaa gagattaaaa tagagtatct cgtgaagtat gttgatcgca ctaagcagag
      961 cggatacctt cttatcacca aaataaaagg caccgattcc tccatctcct tcccagttcc
     1021 agacacgtcc atttacacgt tctatatttt cgaaaacctg attcctgaaa atttcataag
     1081 cagcatcgac ctttggtttg ggattttcct taaccagaac cgagttatcc actatatcaa
     1141 tttttaacaa ggtaataact gcttcttgcc cttctcgaaa aacattccaa tttctggtac
     1201 gctgaacatt cggatcctca acaaagatat ggtttttagg atcatatata agcccgttag
     1261 cacgcatccc ttttaatatc tgatccatat aagatataga gcaaggacgc cccctgtacc
     1321 ccatacggtg aagcttaatc agttcaagaa taaatggagg gaaaaaatgg ttttcgttta
     1381 catccataat tatctgacgt gctatatccc tgttggggat agccatattc tggggaaatc
     1441 cagtctgctt ataaatatca tagttaggca taacctcttt gacaaccgac agcatcatgt
     1501 ccgaaaccat cgattcagcc aaagccttta tgcaaatatt taccagtgct gcttttactt
     1561 tcatatcagt tcccgaatca atctagcgat tctttattaa atagctacaa aaactttcct
     1621 tttttttaac tatttgtcat attatgtcaa gaaaatttaa atagaggtgt agcaatctga
     1681 gaactatttt cgagatgaga gtctctcttg ttcaatttcg gcaaatatct ctcttgtgtc
     1741 tccagttgga tagttaccag aaaaacaggc atcacaaaca ggaagtttta ccttctcgca
     1801 taatttctgc agatcttcaa gtgattgata aattgtagca tctgcaccaa taatttcatt
     1861 aatcttatca attgaaccac agtttgctat caactctccc ttggttgcca tatcaattcc
     1921 atagatgcat ggagatacta ttggtggtgc agcagataca aaataaacct tatcggcacc
     1981 agctttacgt aaaagagaca ctatatgttt tgatgtcgta cctcgaacaa ttgagtcatc
     2041 cacaactgca actttttttc cttttaccac ttcgcgaatt ggattaagct tattacgcac
     2101 aactttttca cgttcactct gtgtaggaga tataaaactg cggccaatat gattgttttt
     2161 tgcaaatcct ctacgataag gtattccaag ctcttcggat aaacctgaag cgaaaaaata
     2221 ggctgaagac ggtacatcaa taactatatc gggctcaaaa ccctcttctc tcattttttc
     2281 cgctaacatg cgtcccattt tcacccttaa atccgcgaca agctgcccac gcataattga
     2341 atcttcacgt gagaaataga tataacagaa gatacaatgt gcagcttttt ccacaaatcc
     2401 ggtgcgtcta tgaacattca gatcattatc tataaagaca atttctccag cacctatatc
     2461 accgataact tcataatcca gataatcgaa acaggtagat tccgaggcaa atccataaca
     2521 cggcccgtct ggtgtatctt tttttcccat tacaagtggt cgtattccat atggatccgt
     2581 aaaaacgagg agccccttgt tggctataac cgtaattgtg gcataggctc cacggacttt
     2641 ctgctgtgta gattttactg aatcaaaaat atcatctata cttagatttt tgagatcatg
     2701 cagctcaagt tcgcttgcaa gtgtatataa aagcagttca agatcatttg atgtttccag
     2761 tagtcgatgg tgctcctcgt agaggtattt tcgtaaataa ttgaaattta tcacgtttcc
     2821 gttatgaacc atagacaatc caaagggata atttaccgta aaaggttgca cgttcataat
     2881 ctcattagaa ccttgagtgg tatatcgaac atgccccatt cctattgatc ctggtaaact
     2941 atcaagatta actttcttga aaacattact tactagtcca agaccttttt taacatgaaa
     3001 tttgtcatca aatgtcacca ctccaacaga atcctgaccg cgatgctgta acgagagcag
     3061 tccggaaata atatcgtaaa ccgccttctt ggaaggcgga ccataaaaac caacaatacc
     3121 gcacatataa acccctcacc aaatcaataa actaatagat attttcaata tcaagcaatc
     3181 aaaacattct ggttaaacat aaaagcggct tatcagccgc ttttatgttt aaccagcaaa
     3241 aattttattt agcatttcac tataaaaaca gtgctcca
//
LOCUS       DAOTGZ010000276         3253 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_3064
            NODE_34894_length_3253_cov_3.617573, whole genome shotgun sequence.
ACCESSION   DAOTGZ010000276 DAOTGZ010000000
VERSION     DAOTGZ010000276.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868088
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 3253)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3253)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:22:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,785
            CDSs (total)                      :: 2,751
            Genes (coding)                    :: 2,732
            CDSs (with protein)               :: 2,732
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 12 of 19
            Pseudo Genes (internal stop)      :: 5 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3253
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_34894_length_3253_cov_3.617573"
                     /isolate="AMR_MDS_3064"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            333..1310
                     /locus_tag="P5123_12805"
     CDS             333..1310
                     /locus_tag="P5123_12805"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRX17189.1"
                     /translation="MSLKFLPISIFLLLLVISCSKTKNNSEANTVNTEASLNDATKEN
                     AEDDYFEKYDVKDDSYIKNKNIICWVDNLRIRKHPDEKSETLDMIKQGEIVYSLGKST
                     QKQFEVELRGIKISGNWEYIMTKQKVKGWVFSGGLSLFSNFKDYNIYGLKDTIFIESK
                     KTQKILYNEKLKAIKDQYINFTSIESDENYLIIYTYYDYGDPEYVSIIVFDMKNNQKV
                     CLIDSVNFYIGRAPSDDYFVFDSGTAANNRIITIFSKNKDMIVFNENPFTYDYSEWKT
                     KGVLYYYHSLGSSVEGKPELKDNYIYAQKYIWKNEEITKTNIIEELYME"
     gene            complement(1426..>3253)
                     /locus_tag="P5123_12810"
     CDS             complement(1426..>3253)
                     /locus_tag="P5123_12810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012414518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016772 - transferase activity,
                     transferring phosphorus-containing groups [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="PEP-utilizing enzyme"
                     /protein_id="HRX17190.1"
                     /translation="AGTRTPNPLDDSTKNSQNKHMPSLEQVMPSIYKQLDDIRTNLEK
                     HYKDMLDIEFTIQDGALYMLQCRVGKRTGVAALNMAMDMLDEKMIDEKTAVLRVDPKQ
                     LDELLHPIIDPAEEKKFTSILKGLPAGPGAASGHVVFTAEDAVAWKKQGEKVILVREE
                     TNPEDVEGMRASAGILTARGGMTSHAALVARGWGKCCVVGAGDMKVNAAAKEAKIGDI
                     FLKEGDVITLNGTRGYVYQGSLPMMDSTEDPRFQKFMSMADKFRAIGVRTNADTPADA
                     KIARDYGAEGIGLFRIEHMFYGEGSDAALFALRKMIFSATKDERVRALSELFPFMKKD
                     IKATLEAMEGCPVTIRLLDPPLHEFVPQSEDKMWEIAKALNISLEEAKKRGQSLHESN
                     PMMGHRGVRLGITYPEITEVQVRAIFESAAELLKSGKKVFPEIMVPVTCGFHETAITR
                     IIVDDVYSQVIKASGLDKIEFKYGTMIEIPRAALRADEMAENAEFFSFGTNDLTQMTF
                     GFSRDDVGSFMNDYIEQGILADDPFQVLDRKGVGQLIKIAVEKGRQTRPDIKIGLCGE
                     HGGDASSIEFLCEVGLNYVSCSPFRVPVARLAAAHANLKTEK"
BASE COUNT         1014 a          691 c          626 g          922 t
ORIGIN      
        1 ataatcatcg acaaatcctt actacaggct gaaacctccg aaagtctgtt ggaaaactcg
       61 aaaaagcttc aatcagttat gtcggttttc aacaagtaaa accatgactg tctatctaat
      121 gaaatttatg tacgcaaaag aatgcaaata caacattagt agttataatt catttaaaac
      181 acatactata ccggagggag ttacgcctga tataaattat gataccgggc attgacacga
      241 tgaatctcat ttaaaagtgt attgacaatt cgatcttcaa taacagatac tattgagatt
      301 tgatgatgag taataaatta ttgaggtaca caatgagttt aaaatttctg ccaataagta
      361 tttttttact tttattagta atttcatgct ctaaaacaaa aaataactcc gaagcaaata
      421 ctgttaatac tgaagccagt ctgaatgacg ctaccaaaga aaatgcagaa gatgattatt
      481 ttgaaaaata tgacgtaaaa gatgattcat atattaaaaa taagaacatt atctgctggg
      541 tagataattt aagaataagg aaacaccctg atgaaaaatc agagacatta gatatgataa
      601 agcagggtga aattgtttac tccttgggaa aaagcacaca aaagcaattt gaagttgagc
      661 ttagaggaat taaaatttct ggcaactggg aatacatcat gacaaaacag aaagtaaagg
      721 gatgggtttt tagcggcggt ctatcgttat tcagtaattt taaagactat aatatatacg
      781 gattaaaaga tactattttt atcgaatcaa aaaaaacaca aaaaatatta tataatgaaa
      841 aattgaaagc aataaaagac caatatatca atttcaccag tatagaatcc gatgaaaatt
      901 atctgataat ttatacgtat tatgattatg gtgatcctga atatgtatct atcatcgttt
      961 tcgacatgaa aaacaaccaa aaagtttgcc ttattgacag tgtaaatttt tatataggaa
     1021 gagcaccttc tgatgattat tttgtatttg atagcggcac agctgccaac aaccgaatta
     1081 tcactatctt ttccaaaaat aaagatatga tagtttttaa cgaaaatccc tttacttacg
     1141 attattcaga atggaaaacc aaaggagtac tctactatta tcattcttta ggttcgagcg
     1201 tagaaggcaa acccgaatta aaagacaact acatatatgc acagaaatat atttggaaaa
     1261 acgaagagat aaccaagaca aatattattg aagaattgta catggaataa aatataattt
     1321 tcgcatacaa atcaaattgg aacatgattg gataggtctt ttgtcacaaa aaaacagacg
     1381 cccatacaga acgcctgttt ttttatactc aaattattta aaaaatcatt tttctgtttt
     1441 aagattcgcg tgagctgcgg caagacgcgc aaccggaaca cggaacggag aacaacttac
     1501 atagttaaga cctacttcac acaggaactc tatcgaagag gcatcaccgc catgttcacc
     1561 gcaaagaccg atcttgatgt caggacgggt ctggcggcct ttttcgacag caatcttgat
     1621 tagctgtccc acccctttcc ggtcaagcac ctgaaaagga tcgtccgcaa gaattccctg
     1681 ctcaatataa tcgttcataa aactgccaac atcgtcacgg ctgaaaccga aagtcatctg
     1741 agtcagatcg tttgttccaa aagagaaaaa ctcggcattt tcggccattt cgtccgcgcg
     1801 taacgcggct cttggaattt cgatcattgt tccgtatttg aattcgattt tgtcaagacc
     1861 ggaagccttg attacctgag aatagacatc atccacaata atacgcgtaa ttgcagtttc
     1921 gtgaaaaccg caggtaaccg gaaccattat ctccgggaaa acttttttgc cggatttcaa
     1981 aagctcggca gccgactcga aaattgcccg gacctgaacc tctgttattt caggatatgt
     2041 aattcccaaa cggacaccgc gatggcccat catggggttg gattcgtgta acgactgacc
     2101 gcgctttttc gcctcttcaa ggcttatatt aagagccttg gcaatctccc acattttgtc
     2161 ttcgctctgc ggaacaaact cgtgcagcgg aggatcaaga agccgtatgg ttacgggaca
     2221 cccctccatt gcttcaagag ttgcctttat atcttttttc ataaacggga aaagttcact
     2281 taatgccctg actctctcat cttttgttgc actgaaaatc atcttgcgca acgcgaaaag
     2341 agccgcgtct gaaccttctc cgtaaaacat atgctctatt ctgaaaagac cgattccctc
     2401 tgctccataa tcacgcgcta ttttggcgtc tgccggagta tccgcgtttg tgcgtactcc
     2461 tattgccctg aacttgtcag ccatagacat aaatttctga aagcggggat cttcggttga
     2521 atccatcatc ggcaaagagc cctgataaac atatcctctt gttccgttaa gggttatgac
     2581 gtcaccttcc ttgagaaata tatcgccaat cttggcctct ttcgcggcag cgttaacctt
     2641 catgtctccg gcaccaacaa cacagcactt tccccagccg cgggctacaa gcgcggcgtg
     2701 tgaagtcata ccgccgcggg cagtaagaat tccagccgat gcccgcatcc cctcaacgtc
     2761 ttcgggattg gtctcttcgc gtacaagaat taccttttca ccctgctttt tccaggccac
     2821 cgcgtcttct gcagtaaaaa ccacatgtcc gcttgcggca ccaggccccg cgggaagacc
     2881 tttaagtatc gaggtgaatt ttttctcttc ggccggatca ataatcgggt gaagcagctc
     2941 gtccagctgt ttcgggtcaa cccgaagaac tgccgttttt tcatctatca tcttctcgtc
     3001 aagcatgtcc atagccatat taagagcggc aacaccggta cgttttccaa cacggcactg
     3061 cagcatgtaa agagctccgt cctgaatggt gaactctata tcaagcatat ccttgtagtg
     3121 tttttcaaga ttggttctga tatcatcgag ctgtttgtat attgagggca taacctgttc
     3181 aaggctgggc atgtgtttgt tctggctgtt ctttgtggag tcgtcaagag ggtttggagt
     3241 tctagtgccc gcg
//
LOCUS       DAOTGZ010000277         3242 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_3064
            NODE_35065_length_3242_cov_4.098525, whole genome shotgun sequence.
ACCESSION   DAOTGZ010000277 DAOTGZ010000000
VERSION     DAOTGZ010000277.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868088
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 3242)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3242)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:22:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,785
            CDSs (total)                      :: 2,751
            Genes (coding)                    :: 2,732
            CDSs (with protein)               :: 2,732
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 12 of 19
            Pseudo Genes (internal stop)      :: 5 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3242
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_35065_length_3242_cov_4.098525"
                     /isolate="AMR_MDS_3064"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..624)
                     /locus_tag="P5123_12815"
     CDS             complement(<1..624)
                     /locus_tag="P5123_12815"
                     /EC_number="3.5.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014968186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016812 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds, in cyclic amides
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyclase family protein"
                     /protein_id="HRX17191.1"
                     /translation="MPQKKNTFIDLSHSITDNMPVHPYDGNVSLCQDKFLHKDKYNNF
                     VLQTGMHAGTHIDTAMHLTESNRYIGQISLESFAGRGVLLDVRGENEIMYRESYADNV
                     KQDDIVILNTGFSEYYGTENYYTDHPVVSEELSHFFVKKKIKMLGIDMPSPDRYPFEI
                     HKKLFSADIHIIENMTNLSELLSVVQFQVFAFPLKIMSEASPARVVAR"
     gene            complement(700..900)
                     /locus_tag="P5123_12820"
     CDS             complement(700..900)
                     /locus_tag="P5123_12820"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRX17192.1"
                     /translation="MKAVKLDISKKYYVLLYIDSAGNFDYETTEGQTPGDYYGMAADV
                     TIPDNCAVSYKNSDTSVFTIVP"
     gene            1482..>3242
                     /locus_tag="P5123_12825"
     CDS             1482..>3242
                     /locus_tag="P5123_12825"
                     /inference="COORDINATES: protein motif:HMM:NF014904.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="[Fe-Fe] hydrogenase large subunit C-terminal
                     domain-containing protein"
                     /protein_id="HRX17193.1"
                     /translation="MTLSAVIAVDADKCVNCHACIAACPVKFCNDASGDHVTLNTDLC
                     IGCGNCITACTHDARYGLDDTDAFINAIDSGENIISIVAPAAAASFTEYERLNTWLIS
                     LGISACFDVSFGAELTVKSYIDYIQKESPKTVISQPCPALVTFIQIYHPELIPYLAPA
                     QSPMLHTVKMIREYYPQYKNYSIVVVSPCYAKRREFDETLPNERVYNLTIKSIESLLQ
                     NSGVNLADLEETPFAGTPAERAVVFSTPGGLLETVRRDVPGIENSTRKIEGVPAIYNY
                     LDNLIDSIKTGESPLLIDALNCHDGCNRGPATVVKDLPLDRIESRINERKSKAAKRYN
                     NKDHKKLSKVSREVNRELNKFWKPGLYNRTYQDLSFLNTVQPVPNNKLKEIFFTMNKN
                     SEKDIINCLACGYNECRKMAKAIYNDLNRRENCFYYTNSMLHQHEEVEEALHAVEDKN
                     SRFREFSQNIESLVNTLSRYMNEVETAMSETSVTIGEIIESNKSASAITAMVNEISFQ
                     TNLLALNAAIEAARAGSMGRGFAVVATEVRDLALKSGNSANEIKTLLEGNAGRIISGD
                     ASVKKTIEAFTKIRGDITLFT"
BASE COUNT         1025 a          660 c          601 g          956 t
ORIGIN      
        1 cctcgcgacc acacgcgcgg gggaggcttc ggacatgatc tttaacggaa acgcgaagac
       61 ttgaaattga acaactgata gcagttcaga aagatttgtc atgttttcga ttatatgtat
      121 atctgctgaa aaaagttttt tatgtatttc gaaaggatat ctatcgggcg acggcatatc
      181 aatacctagc atctttatct tttttttaac aaaaaagtgc gataactctt cacttacaac
      241 gggatgatct gtataatagt tttctgttcc gtaatactca ctgaaaccgg tattgagaat
      301 tacaatatca tcctgtttga cattatcagc atacgattcc ctgtacatga tctcgttttc
      361 tccacgaaca tcaagaagca ctcccctgcc ggcaaagctt tccagagata tctgaccaat
      421 gtaccgattt gattcagtca gatgcatcgc tgtatcgata tgcgtgccgg catgcatgcc
      481 ggtttgcagc acaaaattat tatatttatc tttatgtaaa aatttatcct gacagagact
      541 tacattgccg tcatagggat gaaccggcat attatcggtt atcgagtgac ttagatctat
      601 aaaagtattt tttttctgtg gcacaggttc tccttcttag ataaaccatg caacggttgc
      661 agttttactt aatacctgca accgttattt actccaattc tatggcacaa ttgtaaaaac
      721 tgatgtatcg ctgtttttgt aagagacggc acaattgtca ggaatagtta catcagcggc
      781 cataccatag taatcacctg gggtttgccc ttcagtagtc tcgtaatcga aatttccggc
      841 gctatcaata taaagcaata cataatattt ttttgaaata tcaagtttca ctgctttcat
      901 tgtatcctca tccaaaaaca gagcagatcc tgtgccatca tcagaaatct tgaaagtgca
      961 agtccctaga agatcttttt cattattaat tattatctaa tattcaataa aatcaacaat
     1021 tattttgtaa gtgttatgga tcaatttcat gaaactctga taattaatca aaaaataatt
     1081 gacctttttt atgatttgta cattttttct attcatagaa tattttttgg gaattaattt
     1141 atttcaaaat actttgataa ttatactggt gtatttaaat cataatatat agtctgccca
     1201 gcatttaacc aaattattcg atagcaaaat ttcattttac caacattttt ttgactattc
     1261 tctctttttg attactatta tcacttaaca agattattat ttaacagaaa cgggattgta
     1321 agtctgtata tacaaaaacc ataattgata agtttgcaat aatgcgatta ttaataacgt
     1381 tcattataaa tcgcatgtta accaaacaat aatgcagtaa tggtaatcgt aataactttt
     1441 aatacagtcg agcagtagta taccgtcaac caggaggcca catgacgctc tctgccgtaa
     1501 ttgctgtaga tgccgataaa tgcgttaact gccatgcctg tattgctgcc tgtcctgtga
     1561 aattttgtaa cgacgcctcg ggcgatcatg ttactcttaa caccgatctt tgtataggtt
     1621 gcggtaactg tatcacagca tgcactcatg acgcacgcta cggtttggac gataccgatg
     1681 cttttataaa cgcaattgac agtggagaaa atatcatctc tattgttgca ccggcggctg
     1741 ccgcctcttt taccgaatat gaaaggctaa acacatggct tatatctctg ggtatcagcg
     1801 cctgctttga tgtgtctttc ggagccgagc tgacagttaa atcttatatc gattatatac
     1861 aaaaagagag tcccaagact gttatttctc agccctgccc cgcccttgtc acttttatac
     1921 agatctatca tcccgagttg atcccctatc tggctccagc acagagtccc atgctgcaca
     1981 cagtcaaaat gatcagggaa tattatccac aatacaaaaa ctattctata gttgttgtgt
     2041 caccatgtta tgcgaagaga cgtgaatttg atgagacatt accaaatgaa cgggtatata
     2101 acctaacaat aaaatcaatc gaatcacttt tgcagaactc aggtgttaac cttgcagatc
     2161 tggaagagac tccgtttgcc ggaacacctg ccgaacgggc tgttgttttt tcaacacccg
     2221 gcgggctttt agagacagta cgccgtgatg taccgggaat tgaaaacagc acacgcaaaa
     2281 ttgagggggt tccagcaata tacaactatc tagataacct tattgattct atcaaaaccg
     2341 gggaatcacc gcttttgata gacgccctaa actgtcatga cggttgtaac cgaggaccag
     2401 caacggttgt aaaagatctc ccccttgacc gtatagaatc ccgtataaac gaacggaaat
     2461 caaaagctgc aaagagatat aataataaag atcacaaaaa actttcaaaa gtttcacgcg
     2521 aagtaaaccg tgaactgaac aaattctgga aaccaggtct ttataaccga acctatcagg
     2581 atctctcttt tttaaacaca gtacaaccgg tacctaataa taaacttaaa gaaatatttt
     2641 ttacaatgaa taaaaacagc gaaaaggata ttataaactg tcttgcctgc ggttacaacg
     2701 aatgccgcaa gatggccaag gcgatatata acgatcttaa ccgccgcgaa aactgctttt
     2761 actatacaaa ttcgatgctt catcagcatg aagaggtaga agaggctctg catgcggtag
     2821 aagataagaa cagtcgtttt agagaatttt cacaaaacat tgaatcactc gtcaataccc
     2881 tgagcaggta tatgaatgaa gttgaaaccg cgatgagcga gacgagtgtt accattggag
     2941 aaataattga atcaaacaaa agtgcaagtg ccattaccgc gatggttaac gagatctctt
     3001 ttcaaacaaa ccttttagcc cttaacgcgg caatcgaagc ggctcgtgcc ggtagcatgg
     3061 gtcgaggatt cgccgtagtg gcgaccgagg ttagggatct tgctctaaaa agcggaaact
     3121 cagctaatga aatcaaaacc ctgctcgaag gcaacgccgg tagaatcata tcgggagatg
     3181 cctcggttaa aaaaacaatc gaagctttta cgaagattcg aggagacatt acactattta
     3241 ct
//
LOCUS       DAOTGZ010000278         3237 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_3064
            NODE_35137_length_3237_cov_4.530170, whole genome shotgun sequence.
ACCESSION   DAOTGZ010000278 DAOTGZ010000000
VERSION     DAOTGZ010000278.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868088
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 3237)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3237)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:22:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,785
            CDSs (total)                      :: 2,751
            Genes (coding)                    :: 2,732
            CDSs (with protein)               :: 2,732
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 12 of 19
            Pseudo Genes (internal stop)      :: 5 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3237
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_35137_length_3237_cov_4.530170"
                     /isolate="AMR_MDS_3064"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            140..1456
                     /gene="rodA"
                     /locus_tag="P5123_12830"
     CDS             140..1456
                     /gene="rodA"
                     /locus_tag="P5123_12830"
                     /inference="COORDINATES: protein motif:HMM:TIGR02210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein RodA"
                     /protein_id="HRX17194.1"
                     /translation="MLFRSDFNKLDYVLFIAVIMVIAFGIVTIYSAGYDSVLGVNNNM
                     YKRQIIWFVLGFSIMLGLTFLDYRIFGDYSLQIFGFVLVILILTSFFGPRIRNMRAWI
                     SFGAFSIQPSEFMKIATIIVLAKYLELRERDIYRFKELLVPTVIVLIPMLFIMKQPDF
                     GTAMIFVPVLFAMLFAAGADVTHLVAIILIAVIGLFVPMSLTYYEWVNPESESILLNL
                     YYNGGQLYLVSVVFFVIASVTFLLHLFRVNKYFRRIYIPSYVFSIGLFVSVFFQRYMK
                     DYQKKRILVFLKPDLDPLGSGYNVIQSKIAIGSGGFFGKGFMEGTQTRLGFLPEKTSD
                     FIVAVIAEEWGFVGAMFLLLALFTIVFRGLKISMYTKDKFGALLVIGISALFFVHIII
                     NIGMVIGFMPVTGLPLTFVSYGGSNLLAAMMGVGLLLSVSMHRSSM"
     gene            1547..2713
                     /locus_tag="P5123_12835"
     CDS             1547..2713
                     /locus_tag="P5123_12835"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRX17195.1"
                     /translation="MKILRHILFSLLLLTATQCRTVETLSQPPYESIFSTNRKFDILT
                     SATKGNYRLVLGEAAGYLKDYKFYFAGTSIDSGRLRAIVGSSSEDNLTTVIRDNIKQP
                     VDTYDFLLFLDDLIYHYRGGKLRNKRVWITSGRARRAGILLHPDDVFENRPRNYGENS
                     KTLDIDRPKRPDNLRPANDGDLLGANWSARYKNASTSQKRLIELERKNPDFTKRTRLL
                     LNQLEKQGALVAVHSAYRKRERGYLMWGAFLLSRSKSKKETERIISELEKVNLNNKLN
                     VRIVWMDPKGWQQTVKRAKEMADAYDVVYATKHGALKSDHYDSKAVDITATGLPARLK
                     LVSPSGVKKIFDLSGENQSRDLNLTPEIIDWVEKNFYFKKLRSDYPHWSDEKKI"
     gene            2772..>3237
                     /locus_tag="P5123_12840"
     CDS             2772..>3237
                     /locus_tag="P5123_12840"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRX17196.1"
                     /translation="MIANNLWDFLSPVNCAICGNEYSRNDGFICLSCRNKLKLAVYKA
                     DPAEYLSKRYFYFDLLISLYDYDDMVKPLIISFKERDRVELADFVAESASNNAINGLN
                     ADIITAVPLSRSKEFARGYNQSELFAKSLSKYSGISYLPMLACRGKREVQKKM"
BASE COUNT          931 a          539 c          684 g         1083 t
ORIGIN      
        1 ggttatggac cgcatggagc tgatccacga gacgcttatg ttattatggt aatggtcgaa
       61 tacggtattg cgggggctgc tacagcggtt cctgtcgcag aaaaggtgtt ttctaaaatg
      121 atagaacagg gatatttcga tgttgttcag aagtgatttt aacaaactgg attatgtgtt
      181 atttatagct gtaataatgg ttatagcttt tggtattgtt acgatatata gcgcaggata
      241 cgactctgtt ttaggtgtta ataataacat gtataagcgc cagataatct ggttcgtttt
      301 gggtttttca attatgcttg ggttaacatt tctagattac agaatatttg gtgattattc
      361 gctgcaaata tttggttttg tgttggttat ccttatattg acatcatttt tcggcccacg
      421 tatacgtaat atgcgagcct ggatcagttt tggagcgttt tccattcagc cttcagagtt
      481 tatgaaaata gcgacaatta tagttttggc aaaatatctt gagctgcgtg aacgtgatat
      541 atatcgattc aaagagcttc ttgttccaac tgtcattgtt cttataccta tgctttttat
      601 tatgaaacag cctgattttg gaactgcgat gatttttgtt cctgttcttt ttgctatgct
      661 ctttgctgcc ggtgccgatg ttacgcatct tgtagcaatt attctgatag ctgttattgg
      721 actttttgtc cctatgtcgc taacctatta cgaatgggta aatcccgaat cagaaagtat
      781 tttgcttaat ctatattata atggtggtca gctttatctg gtctccgttg ttttcttcgt
      841 tattgcttcc gttacttttc ttcttcatct ttttcgtgta aataaatatt tcagaagaat
      901 atatatcccc tcgtatgtct tttcaattgg tctgtttgta tctgtttttt ttcaaagata
      961 tatgaaagat tatcagaaaa aaaggattct ggttttttta aagccagatc ttgatccctt
     1021 agggtctgga tataatgtta ttcagtcaaa aattgccata ggttcgggag ggttttttgg
     1081 taaaggattt atggagggaa cacagactcg tttggggttt ttgcctgaaa aaacttcaga
     1141 ttttattgtt gccgtaatcg ccgaggagtg ggggtttgta ggagcaatgt tcttattatt
     1201 ggctctattc acgatagttt ttagaggttt gaaaatttcg atgtatacca aagataagtt
     1261 cggagcatta ttggttatag gaatttcggc tttattcttt gttcacatta taattaatat
     1321 aggcatggta atcggtttta tgccagttac cggcttacca ctgacttttg tgtcttacgg
     1381 tggttcaaat cttcttgctg ccatgatggg ggttgggctt ctcctttcag taagcatgca
     1441 taggtcaagt atgtaacgga tcttctttta acatagaaac agttttttat gaaatattcc
     1501 tgcttcacga tctgttgcag catatctttg aactcaggac attaacatga aaatacttcg
     1561 ccatatatta ttttccctat tgctgttaac agcgacgcaa tgtcgaactg tcgaaacctt
     1621 atcccaacca ccctacgaga gcattttttc tactaaccgt aagtttgaca tcttgacatc
     1681 ggccacgaaa ggtaattata gattggttct tggcgaggcc gccggatatc ttaaagatta
     1741 caagttctac ttcgccggta caagcataga ctccggtaga ttaagagcaa ttgttggatc
     1801 atctagtgag gataatctga caacagttat acgggataat atcaagcaac ctgtagatac
     1861 ttatgatttt cttctttttc ttgatgatct tatataccac tatcgaggtg ggaagctgcg
     1921 caacaagcgt gtatggataa catcaggcag agcgcgacgt gcaggaatct tattgcatcc
     1981 tgatgatgta tttgaaaaca gaccacgaaa ttatggtgaa aattctaaaa cattggatat
     2041 agaccgcccc aaacgaccgg ataatcttcg tcctgctaat gatggcgatc ttttaggcgc
     2101 taattggagc gctcgttaca aaaatgcgtc taccagtcaa aagagattaa tcgagttaga
     2161 acggaaaaat ccggatttta ccaaaagaac tcgattactg ttaaatcagc ttgaaaaaca
     2221 aggtgcactt gttgctgttc attcagctta tcgtaaacga gaaaggggat accttatgtg
     2281 gggtgccttc ctgctaagcc gttccaaaag caaaaaagag acagaacgaa tcatatcgga
     2341 actagaaaag gtgaatctta ataataaact gaatgtgcga attgtctgga tggatcccaa
     2401 aggctggcag cagactgtaa aaagagcgaa agagatggcc gacgcttacg atgtggttta
     2461 cgcgaccaaa catggggcat taaagtcgga ccattatgac tcaaaggcag ttgatattac
     2521 agcaacgggt ttaccagcca ggttaaaact ggtatcccca tcaggtgtta aaaaaatttt
     2581 cgatttgtca ggtgaaaatc agtcaaggga tctgaatctt acaccggaaa tcatagattg
     2641 ggtagaaaaa aatttttatt ttaaaaaatt acgtagtgat tatccgcatt ggagtgatga
     2701 aaaaaaaatt taataaaaag taaaaaaatc agtctatata gttagatatc taatttgtat
     2761 cggaggctga tatgattgct aataatctat gggattttct ttctcctgta aactgtgcga
     2821 tatgcggaaa tgaatatagt cgcaatgacg gattcatttg cttatcttgc cgaaataaac
     2881 ttaagttggc cgtctataag gctgatcctg ctgaatattt gtcaaaaaga tacttctatt
     2941 tcgatctgct aatctctctt tatgattacg atgatatggt aaaaccattg attatttcgt
     3001 ttaaagagag ggatcgtgtt gaattggctg attttgttgc cgaatctgca tctaataatg
     3061 ctataaatgg tttaaatgcg gacattatta ctgctgttcc attatccaga tccaaagagt
     3121 ttgcgcgtgg atataatcaa tctgagttgt ttgccaagtc cctgtcaaaa tacagtggta
     3181 taagctattt accaatgctc gcatgtaggg gaaagcgtga ggttcaaaaa aaaatga
//
LOCUS       DAOTGZ010000279         3190 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_3064
            NODE_35841_length_3190_cov_5.792026, whole genome shotgun sequence.
ACCESSION   DAOTGZ010000279 DAOTGZ010000000
VERSION     DAOTGZ010000279.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868088
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 3190)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3190)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:22:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,785
            CDSs (total)                      :: 2,751
            Genes (coding)                    :: 2,732
            CDSs (with protein)               :: 2,732
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 12 of 19
            Pseudo Genes (internal stop)      :: 5 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3190
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_35841_length_3190_cov_5.792026"
                     /isolate="AMR_MDS_3064"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1280
                     /locus_tag="P5123_12845"
     CDS             <1..1280
                     /locus_tag="P5123_12845"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HRX17197.1"
                     /translation="SSSVQENLNGIRVVRAFAQQNYENRMFETKNRAQLNAGLKEVFW
                     HSSYWPVAHILCGTQLCVSILYTGYTVINGENTVGTLVAASFLFNGLIWPLQDLGRLI
                     TEISHSFVSFNRIEEIMTEETEDTENNISRDNKLIQSDNKPLSGRYNNHSESANDSNN
                     FKLPLRLEGHIEFENLCFSYGDTAPVLKNISFECKPGQKIALVGETGSGKTSIVNLLP
                     RFYPYQSGNLLIDRIPVTDYPRNYLRKNIGIVEQNPFLFTTTIRENIEYGTDRDIDFE
                     EVVKAAKAAAIHNDIMRFPLKYDTVVGEKGISLSGGQKQRIVIARTILKDPHILILDD
                     STSAVDADTEQCIRKAINNLLKGRSTFIIAHRIQTLKEADMIIVLKEGEIKQQGSHDE
                     LIKVPGFYKDIFEIQTRIEKELILEIESNRKGK"
     gene            1283..3091
                     /locus_tag="P5123_12850"
     CDS             1283..3091
                     /locus_tag="P5123_12850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013560663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HRX17198.1"
                     /translation="MSIKQKKESREVELFKEHYKGKLYRRLLIISLTKWRLLAGFLTA
                     IVITAFIDSTNTYISKFIIDDGVIPGNTQTMFKYGIIMFVLYLLNSMAIFTFIFCAGR
                     LGEHMQYHLRKEQFSHLQKLSFSFFDRVSSGWLLSRITSDSRRIAELCTWMFLDMTWG
                     AVNIVVALIFMATINLKLMFVVALMTPILVIASIKFRSLILKEYRKVRSINSQITSAY
                     SENISGVRIVKALNREEKNLANFSKITRQMHKRSFRAGWLSAFFLPVVQMITTLAVAA
                     ILYVGGSDISTGALSVGELRAFIGYVLFMMWPIENLSRVFSEMQRSIASAERVFSLLD
                     TPSGIADRKGSHTTANFKGKIDFCSVSFHYEKDSPVLKNINLSVMPGEVIAIVGPTGG
                     GKSTLVNLAARFYEPCKGKILFDGEDYRNWTQEALQHRIGIVLQDPHLFSGTIRENIL
                     YGKPDATEEEIIAAASRVQAHEMICRLPNGYQENVGEAGNLLSIGQKQLISLARTIIS
                     EPDIIIMDEATSSIDSLTEHLIQEGIHHLMEGRTSFVIAHRLSTIREANRILVLESGQ
                     IKESGSHDELLKNRGHYYELYTSQIRSEKSGCAQLA"
BASE COUNT         1021 a          612 c          667 g          890 t
ORIGIN      
        1 tatctagctc ggttcaggaa aatcttaacg gtataagagt agtacgcgcc tttgctcaac
       61 aaaactatga aaaccgtatg tttgaaacta agaaccgcgc ccaattgaac gccggtttga
      121 aagaagtttt ctggcactca tcttactggc cggtagccca cattctatgt ggcacacaac
      181 tttgtgtatc aattctttac acaggttata cagttattaa tggcgaaaat accgtaggta
      241 ctttggtcgc agcaagtttt ctgtttaatg gccttatctg gccattacaa gatttgggca
      301 gattaatcac tgaaataagt cacagttttg tatcttttaa cagaattgaa gagatcatga
      361 cagaagagac cgaggacaca gagaataaca tttcacggga taacaagctg attcaaagcg
      421 ataacaaacc actatccggc agatacaaca atcattctga atctgccaac gacagtaaca
      481 attttaaact gcctctgcga ctggagggtc atatagaatt tgagaatcta tgtttttcgt
      541 atggtgatac ggctcctgtt ttgaagaaca tctcttttga atgtaagccg ggacagaaaa
      601 tagcactggt tggggaaacc ggatctggta aaacttcaat agtaaatctg ttgccccgtt
      661 tttatcctta ccagagcgga aatctattga tagacaggat acctgtaaca gattacccca
      721 gaaattattt acgtaaaaat atcggtattg ttgaacagaa tccgtttctg tttactacaa
      781 caattcgtga aaatatcgaa tacggtacag atcgtgatat tgattttgag gaggtggtca
      841 aagccgcaaa agctgccgcc atacataatg atattatgag atttcctcta aaatacgata
      901 cagtagttgg agaaaaaggt ataagcctct ccggaggaca aaaacaacgt attgtgatag
      961 cccgaacaat attgaaggat ccgcatatcc tgattctgga tgattcgaca tcggctgttg
     1021 acgcggacac cgaacagtgc attcgcaaag ctattaataa tctgttaaag gggcgatcaa
     1081 cctttattat cgcacataga atacagaccc ttaaagaggc cgatatgata atcgtattaa
     1141 aagaagggga aataaaacag caaggcagcc atgatgaact aatcaaggta cctggcttct
     1201 acaaggatat tttcgagata cagaccagaa ttgaaaaaga actgattctt gaaatcgaaa
     1261 gcaacaggaa aggtaaataa taatgagcat taaacagaaa aaggaaagtc gtgaagttga
     1321 acttttcaag gaacattaca agggtaaact gtacagaaga cttctaataa tctcacttac
     1381 aaagtggaga ttattagccg gctttttgac cgccattgtc ataacggctt tcattgattc
     1441 gacaaacaca tatataagta aatttattat cgatgatggt gtgattccag gaaacactca
     1501 aaccatgttt aaatatggca taatcatgtt tgtattgtat ctcttaaaca gtatggccat
     1561 ttttacattt atcttttgcg ccggccgcct tggagaacat atgcaatacc atcttcgaaa
     1621 agagcagttt agtcatttgc aaaaactttc tttttctttt tttgaccgcg tatctagcgg
     1681 atggcttttg tcaagaataa ccagcgactc aagaagaatt gcagagctgt gtacctggat
     1741 gtttcttgat atgacatggg gagctgtaaa tattgtggtt gctcttatct ttatggccac
     1801 tattaatttg aaacttatgt ttgtcgttgc actgatgact ccgatactgg ttattgcctc
     1861 gataaaattc cgttcactta ttttaaaaga gtaccggaaa gtacgttcta ttaattcaca
     1921 gataaccagt gcctactccg aaaacatatc aggagttcgc atcgtcaaag ctcttaaccg
     1981 tgaagagaaa aatcttgcaa atttttccaa aataaccaga caaatgcaca agcgttcttt
     2041 tagagccggc tggctttccg cattttttct gccggtagtt caaatgatta ccactcttgc
     2101 ggtcgcagct attctttacg tgggtggatc agatataagt acaggagctc tctctgttgg
     2161 agagctgcgc gcgtttatcg gatatgtact tttcatgatg tggccgatag aaaacctttc
     2221 acgggtcttt agcgaaatgc aaaggtcgat agccagtgcc gaacgtgttt tttcacttct
     2281 tgatacacct tctggaatag cagaccgcaa aggaagccat acaacggcaa atttcaaagg
     2341 aaaaattgat ttttgttcgg tcagctttca ttacgaaaaa gactcccctg ttttaaagaa
     2401 catcaatctt tcggtcatgc cgggcgaggt aatcgcaata gttgggccaa caggaggtgg
     2461 gaaaagcaca cttgtaaacc ttgcagccag attttacgaa ccgtgtaaag gcaaaatact
     2521 ttttgacggt gaggattacc gtaactggac acaagaggct cttcaacatc gcataggtat
     2581 agttcttcag gatccgcatc tgttttccgg aacaatacgt gagaatattt tgtacgggaa
     2641 accagacgcg actgaagaag agataatcgc agctgcatca cgagtgcaag ctcacgaaat
     2701 gatttgtcgt cttccaaacg gatatcagga aaatgtcggt gaggcaggga accttttatc
     2761 aataggtcag aaacaactga taagcctcgc aagaacaata atatccgagc ccgatattat
     2821 aataatggac gaggccacaa gctctattga ttctttgacc gaacacctga ttcaggaggg
     2881 cattcatcat ctgatggaag gtcgaaccag ctttgttata gcacacaggc tctctacaat
     2941 aagggaagct aaccggatac tggttcttga atccggacaa ataaaggaga gtggaagcca
     3001 cgacgagctg ctaaagaatc gaggacacta ttatgagctt tacacaagtc aaatacgttc
     3061 tgaaaaatcg ggatgcgccc aattagcttg agaatagtca gctccctttt atgaaaagtt
     3121 attgattaat tatcaaaggc tttttatgaa tgtgttaaat gaaagagacc tattttataa
     3181 aactatatag
//
LOCUS       DAOTGZ010000280         3178 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_3064
            NODE_36014_length_3178_cov_4.398655, whole genome shotgun sequence.
ACCESSION   DAOTGZ010000280 DAOTGZ010000000
VERSION     DAOTGZ010000280.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868088
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 3178)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3178)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:22:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,785
            CDSs (total)                      :: 2,751
            Genes (coding)                    :: 2,732
            CDSs (with protein)               :: 2,732
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 12 of 19
            Pseudo Genes (internal stop)      :: 5 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3178
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_36014_length_3178_cov_4.398655"
                     /isolate="AMR_MDS_3064"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            32..1270
                     /locus_tag="P5123_12855"
                     /pseudo
     CDS             32..1270
                     /locus_tag="P5123_12855"
                     /inference="COORDINATES: protein motif:HMM:NF012973.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_process: GO:0009306 - protein secretion [Evidence IEA];
                     GO_process: GO:0044780 - bacterial-type flagellum assembly
                     [Evidence IEA];
                     GO_process: GO:0071978 - bacterial-type
                     flagellum-dependent swarming motility [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthesis protein FlhA"
     gene            1289..2665
                     /gene="flhF"
                     /locus_tag="P5123_12860"
     CDS             1289..2665
                     /gene="flhF"
                     /locus_tag="P5123_12860"
                     /inference="COORDINATES: protein motif:HMM:TIGR03499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006614 - SRP-dependent cotranslational
                     protein targeting to membrane [Evidence IEA];
                     GO_process: GO:0044781 - bacterial-type flagellum
                     organization [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthesis protein FlhF"
                     /protein_id="HRX17199.1"
                     /translation="MKYVKLTAENYNEALKQLREKYGSDAIPISHKNVKEGGLFNNRF
                     FAKEIVELMAAIPEPSQQRPVARKSILDVKVDNDVSDAFSKAGYGNGYNQSESRQPFT
                     RNSSAIRDAEKEIRREAAERQSEFFNKTVSPFDNNGLSGVNGLNSAKKEDVVEISPEE
                     YEKLKNFEKEFHDVKANLDRIMQNQKEYQKTEKKEETGFFSDYMNILKENEYTDDERS
                     ELISAVKKSLSEDDRNDKSLIEKSLKDLIKSKIVTSGPVCHSGRKKIVMFVGPTGVGK
                     TTSLAKMGAHLSLRDKKKVAFITIDTYRIAATEQLKKYAEIMRIPIHVVNDKKELKKV
                     IDHEDADVILIDTSGRSHKNTMKISEIKNFADAVDYDFEKILCVSASTKRTDAESIFN
                     AFKPVDCNSVLITKVDETSFVGNIVKVADKYNKPISYIANGQEVPNDLYAADSQNLAD
                     MIVNGTPQ"
     gene            2684..>3178
                     /locus_tag="P5123_12865"
     CDS             2684..>3178
                     /locus_tag="P5123_12865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010569452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="P-loop NTPase"
                     /protein_id="HRX17200.1"
                     /translation="MDQASNLRKLVVAKNDNKKRTRTIAITSGKGGVGKTSLSVSLAI
                     ALARDKSTVTLLDADLGLANVNVVLGIIPKYNLYHVIKGKKRLNEVIVSVPEGIRIIA
                     GAPGYHQLANLDEKARQKFITQLGDMENDDYLIVDTGAGVSQNVLSFVTSADEIIVVT
                     TPEPT"
BASE COUNT         1063 a          539 c          709 g          867 t
ORIGIN      
        1 gatagcaaaa tagttcagtg cacaacctcg agctcttatc attggcgcgg tagctttagg
       61 tgcttctgct cttatacccg gatttcccaa gatgtccatt attacactct ctcttgctct
      121 tggttttttg gctatgtttt tacaacgtag tcaaaatgaa acagccgaag agattcgtcg
      181 taccgagaca gaaaaaagtg gtgccgaaaa accggaatct gttgttccct atgtttcggt
      241 tgatccactg gaggtcgaga ttggctataa tctgattcct cttgtcgacc ctaaacaagg
      301 gggaactctt cttgaccgta ttacaaagat tcgtcgtatg agtgcgttaa acatgggtct
      361 tattgttcct ccaatacgaa taagggataa tatggagtta gcccctgaat cttactcgat
      421 tctaatcaag ggtgtggata tcggcaacgg aacattgatg gtcggaaaat taatggcaat
      481 ggacaccgga gaggtagttg ataaaataga gggacaggaa ttcatagaac caacatttgg
      541 actaaatgct atatggatag acgcagataa gagggatttt gctgagagaa acggatacat
      601 agttgtcgat tgcccgacta taattgcgac tcacctgaca gaaataataa aacgtcatgc
      661 tgatgagata cttggaagac aagaagttca gcaacttata gacaatatta aaaatgatta
      721 ccctgctgtt gttagtgata taatggataa aaacaagatg tcattgtcag ttgttcagcg
      781 tgttttgcaa aatcttttaa gagaacgcgt gtccatacgt aatcttgtta caatttttga
      841 aactcttgca aattaccagg attacaccac tgatccatcc atgcttacag aatatgtgcg
      901 cgctgcattg gcacgacaga tatgtaatga ttacagtgat aagggaacaa tgtatgcaat
      961 aactgttgat cctgaaatgg aaggaataat ccgggaagga attcatgacg atccggttga
     1021 aggtcgtgtt gtgggactgg atcctcagac tcataccatg gttataaacg cacttcttga
     1081 agcctataaa agctgcaaac agatgggata tgctccagta tatcttgttt cgccgcatat
     1141 aagatcggtt ctttttgctc ttcttgagcg ggaagtaagc gatgcagttg tattgtctta
     1201 caatgagatt acttctaata taaatgttaa tgttgtttcg tctgccatgt tgcagtcggc
     1261 tacagcataa taaaggggag aaaaaaagat gaaatatgta aaattaactg ctgaaaacta
     1321 taatgaggct cttaaacagc ttagagaaaa atacggctct gacgcgatac ctataagtca
     1381 taaaaacgtg aaagaggggg ggctttttaa caacagattt ttcgcaaaag aaattgttga
     1441 actgatggca gcaattccgg aaccttcaca acaacgtccg gtggctcgta aaagtattct
     1501 tgacgtcaag gtagataatg atgtctctga cgctttttca aaagcagggt atggtaacgg
     1561 ttataaccag agtgaatccc gtcaaccttt cactagaaat agtagcgcaa tacgtgatgc
     1621 cgaaaaagaa atcaggcgtg aagctgctga acgtcaatct gaatttttta ataagacagt
     1681 ctccccattt gataataatg gattatcagg tgttaatgga ttaaacagtg ccaaaaaaga
     1741 ggatgtagtt gaaatatctc cggaagagta tgaaaagctt aaaaattttg aaaaagaatt
     1801 tcatgatgtt aaagctaact tggacagaat tatgcagaat caaaaggagt atcagaagac
     1861 cgaaaaaaaa gaagaaacag gtttttttag cgattatatg aatattttaa aagaaaacga
     1921 gtatacagat gatgagagat cggaactgat cagtgcggtt aagaaaagcc tgagcgaaga
     1981 cgatcgtaat gataaatcct tgatagaaaa gagtcttaag gatctaataa aaagtaaaat
     2041 agtaacatcc ggtccggtgt gccatagtgg tcggaaaaaa atcgttatgt ttgtggggcc
     2101 taccggggtt gggaaaacca catcattggc taaaatggga gcccatcttt cgctgcgcga
     2161 caaaaagaag gttgcattta ttacaataga cacatatcga atagccgcta ctgaacagtt
     2221 gaaaaagtac gcggaaatca tgcgtatacc gattcatgtt gttaatgata agaaagagct
     2281 aaaaaaagtt atagaccatg aggacgcgga tgttatttta attgatactt ccggccgttc
     2341 acataaaaat acgatgaaaa tttccgaaat taaaaatttt gctgacgcag tggattatga
     2401 ttttgaaaaa atactctgtg taagcgccag cactaaacga acagatgcgg aaagtatttt
     2461 caatgccttt aaaccagtag attgcaacag tgttttaata acaaaagtcg atgaaacttc
     2521 ttttgttggg aatatcgtga aagttgccga taaatataac aagccgatct cttatatagc
     2581 gaacggccag gaagtaccaa atgatctgta cgcggcagac tcgcaaaatc ttgccgatat
     2641 gattgttaat ggtactcctc aataagagtg atggagaaat agagtggatc aggcatctaa
     2701 tttacgaaaa cttgttgttg ccaaaaacga taataaaaaa aggacacgca cgatagccat
     2761 aaccagtggc aagggtggtg ttgggaaaac gagtctttct gttagcctag cgatagcact
     2821 agcgcgtgac aagagtaccg ttactctttt ggatgctgat ctgggcttgg caaacgttaa
     2881 cgtcgttttg ggaattattc ccaaatataa cctttatcat gtaataaaag gtaaaaaacg
     2941 tcttaacgag gtaattgtat ccgtacccga aggaataaga attattgccg gagcaccggg
     3001 atatcatcaa ctcgcaaatc tggatgaaaa ggcaagacaa aagtttataa cacagcttgg
     3061 cgatatggaa aacgatgatt atctaatagt tgatacgggt gccggtgtgt ctcagaatgt
     3121 gctaagtttt gtaacgtctg ctgatgaaat tattgttgta acaactcctg agccaaca
//
LOCUS       DAOTGZ010000281         3174 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_3064
            NODE_36073_length_3174_cov_5.667522, whole genome shotgun sequence.
ACCESSION   DAOTGZ010000281 DAOTGZ010000000
VERSION     DAOTGZ010000281.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868088
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 3174)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3174)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:22:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,785
            CDSs (total)                      :: 2,751
            Genes (coding)                    :: 2,732
            CDSs (with protein)               :: 2,732
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 12 of 19
            Pseudo Genes (internal stop)      :: 5 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3174
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_36073_length_3174_cov_5.667522"
                     /isolate="AMR_MDS_3064"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..753)
                     /gene="rsmG"
                     /locus_tag="P5123_12870"
     CDS             complement(<1..753)
                     /gene="rsmG"
                     /locus_tag="P5123_12870"
                     /EC_number="2.1.1.170"
                     /inference="COORDINATES: protein motif:HMM:TIGR00138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005575 - cellular_component [Evidence
                     IEA];
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0031167 - rRNA methylation [Evidence IEA];
                     GO_process: GO:0046118 - 7-methylguanosine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(527)-N(7))-methyltransferase
                     RsmG"
                     /protein_id="HRX17201.1"
                     /translation="MVINPTERDYFFSTINQTFKHLGEDVTQNLFKFCEMLVQYNDEY
                     DLSRLKKFKDILEKHFLDSIIISDLINLPESLLDIGTGAGFPGIPLKITQPELQLTLA
                     EPRPLRVKFMRMVIEELKLDSTSVFPHKVNEKMYFPIQGVITRAFESAPATLERCINF
                     LPDGGSVILLKGPGVDEEINDISPIMHEHYRLEKDIPYSLPGTDHKRRLIIYKKTSNY
                     CRRFYQISDIKELTAGTVIESTDNKRYKELKKA"
     gene            complement(747..1694)
                     /locus_tag="P5123_12875"
     CDS             complement(747..1694)
                     /locus_tag="P5123_12875"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: protein motif:HMM:NF013700.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M23 family metallopeptidase"
                     /protein_id="HRX17202.1"
                     /translation="MKLNIFILLFNVFFCLLLTVPLPAIVSKSAIKKDELKLLPISHY
                     YDNETNYILSINNSIRFENVSFSKYTIVPGDNFWKIAKDFNVNIDTLIAANPFWNSLL
                     AVTGQTIVVPSKKGALVFIDKTTTLEELANHYEVDITDIEIQQISNFSKYHDRFGTLP
                     FGIAVFIHGSKPSVETMTEELAHQYTLREMFRSPLGGRFTSFFGNRRHPIFNTRKFHS
                     GVDIAARRGTYVGAASPGKVIAAGWMGGYGKAVIVQHKNGYKTLYGHLSVICTKKGRH
                     VKSGQLIGRVGSTGYSTGPHLHFTLWHNEKLINPMDVLW"
     gene            1932..>3174
                     /locus_tag="P5123_12880"
     CDS             1932..>3174
                     /locus_tag="P5123_12880"
                     /inference="COORDINATES: protein motif:HMM:NF024116.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HRX17203.1"
                     /translation="MNLYELFIGFRYLKSKKHQAFISFNTLLSVGIVFLGVFILVLVI
                     SVMNGFQAQIKDKILDIDSHITINGYDGINFSAGIDDYENVTKKILANPDVVSAEPFF
                     QEEGILSFNRKISGVMIRGIGSEEGIPENIEKFIDEGQMGMENPQRGFTGPNQVFIGK
                     ELSSLKQIYIDDTIEIIVAKQNQTDGLSGFASSAPRIEKLKVVGYFKTGYYDFDTKML
                     ITSLPTTQKIYGYEGKAWGIGVKVRDIYQVNRIAAELQEMLGFDYQTFTVEDRNENLF
                     QALNIERLVMTVILFLTIISAGFTIMGTLVMVVMEKRKAIGILKSLGAKPSSIMTIFI
                     LEGFLIGVIGTVAGLFTGIAAALNLEQIIISIENFINNAGEWLYTAFNLGIWINKHIV
                     PTHVYYIDAIPTEIKPGFVIFI"
BASE COUNT          927 a          531 c          667 g         1049 t
ORIGIN      
        1 ggcttttttt aactccttat agcgtttgtt atcagtagat tcaattacag ttcccgctgt
       61 aagctctttt atatcagata tctgatagaa tcgcctacag taattacttg ttttcttata
      121 tataattaaa cgacgcttgt gatcggtacc aggaagggag tagggaatgt ctttttcgag
      181 cctgtaatgt tcgtgcataa tgggtgagat gtcattgatc tcttcatcaa ctccaggacc
      241 tttaagaaga ataaccgagc ccccatcggg caaaaaatta atacaacgtt ccagtgttgc
      301 cggggcggac tcgaaggctc tggtgattac cccttggatc ggaaagtaca ttttttcgtt
      361 aaccttgtgg gggaaaaccg aagtgctatc aagttttagc tcttcgataa ccattctcat
      421 aaattttacg cgaagtggtc gtggttcagc cagtgtaagt tgtaattcgg gttgagttat
      481 ttttaacgga atacctggaa atccggctcc agttccaata tccagaagag attctggtaa
      541 atttatcagg tcgctgatga ttatagagtc aagaaaatgt ttttccaaaa tatctttgaa
      601 tttttttaaa cgtgaaagat catactcgtc attatattga acaagcattt cacaaaattt
      661 aaacagattt tgtgtaacgt cctctccaag atgtttaaaa gtttggttta ttgtggaaaa
      721 aaaataatcc cgttctgtcg ggttgatcac cataatacat ccattggatt aattaatttt
      781 tcgttatgcc aaagggtaaa atgaagatga ggtccggttg aatagccagt cgaaccgact
      841 cttcctataa gctggcctga tttaacatgg cgtcctttct ttgtgcatat taccgaaagg
      901 tggccataga gagttttata gccgttttta tgttgaacta ttacggcttt cccgtaacct
      961 cccatccagc ctgctgctat aaccttgcct ggacttgctg cgcccacgta tgttccgcgt
     1021 cttgctgcta tatcgacccc actgtgaaat ttgcgggtat taaatatagg atgacgtctg
     1081 tttccgaaaa acgaggtgaa ccttccacct agtggagatc gaaacatttc cctgagtgtg
     1141 tattgatgag cgagctcttc ggtcatggtt tcgactgatg gcttagaacc gtgaataaaa
     1201 actgctatac cgaaaggaag tgtgccaaat cggtcatggt atttggaaaa attgctgatt
     1261 tgttgtatct ctatatcggt tatatctacc tcatagtggt ttgctagttc ttcaagggtt
     1321 gtcgttttgt ctataaatac cagagcacct ttttttgatg gtacgactat tgtttgacct
     1381 gtgactgcaa gcaaagagtt ccaaaatgga ttagccgcaa taagggtatc aatatttaca
     1441 ttaaagtctt tggcaatttt ccagaaattg tctcctggca ctatggtgta tttggaaaaa
     1501 gagacatttt cgaatcgtat actattgttg atgcttaaaa tatagttggt ctcattatcg
     1561 tagtagtgac ttattggcaa aagcttcagc tcgtcttttt ttatggccga ttttgagact
     1621 attgccggaa gtggaacagt taacaaaaga caaaagaata cattaaaaag caaaatgaat
     1681 atatttaatt tcatagtcaa acaacctcgt atgtttaaga cattgtcgta aggtaataaa
     1741 aaaggtcaag cagaaatggt tttgtacatc cctgattaat tatcggatga ttgcctgaat
     1801 aggttaattg tcatagtttc agatatgtga tgctcaagcc tgattacacg gtcttttttc
     1861 cttgacgtag gcaactgtca aatataataa caggtgactt gcctgcttac aataagtcaa
     1921 tggagtagag aatgaatctt tacgagcttt ttataggatt tcgttattta aaatcaaaaa
     1981 aacatcaggc ttttatatct tttaatactc ttttatcagt tggtattgtt tttcttggag
     2041 tttttattct agttcttgtg atttctgtta tgaacggttt tcaggcacag ataaaggaca
     2101 agatacttga tattgattca catataacta ttaatgggta tgacggtatc aattttagtg
     2161 ctggcattga tgactacgag aacgtcacga agaagattct tgcaaatcca gatgtggttt
     2221 ctgctgaacc tttttttcag gaggagggaa tcttaagttt taatcgtaaa atttcgggag
     2281 taatgattcg cggtataggt tctgaagagg gaattcccga aaatattgaa aaatttatag
     2341 atgaaggtca aatggggatg gaaaatcctc agcggggatt tacagggccg aatcaggttt
     2401 ttataggaaa ggagttatcc tcattaaagc agatttatat tgatgataca atagagatca
     2461 ttgttgccaa acagaatcag actgacggtt tatccggttt tgcctcatct gctccgcgta
     2521 ttgaaaaact caaggttgtt ggttatttca aaaccggata ttacgatttt gacacaaaga
     2581 tgcttataac atcgttgcct acgactcaaa aaatatacgg atatgagggt aaagcttggg
     2641 ggataggcgt taaggttcga gacatatatc aggtaaaccg tattgcggca gagttgcagg
     2701 agatgcttgg ctttgactat cagactttta cagtcgaaga tcgtaatgaa aaccttttcc
     2761 aggcactaaa tattgagaga cttgtaatga cggtgatact ttttttaacc ataatatcag
     2821 caggttttac aataatggga acactggtta tggttgttat ggagaaacga aaggcaattg
     2881 gtattctcaa atcgcttggc gctaagcctt cttcgataat gacaattttt attcttgaag
     2941 gttttttaat aggagttatt ggaactgtcg ctgggctttt tactggtata gccgcagctt
     3001 tgaatcttga acagataatt atttcaatag agaatttcat taataatgct ggagaatggc
     3061 tttatacggc atttaatctg ggtatctgga ttaataaaca cattgtgccg actcatgtct
     3121 actatattga cgctataccg acagagataa aacccggttt tgtaatcttt attg
//
LOCUS       DAOTGZ010000282         3154 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_3064
            NODE_36383_length_3154_cov_3.960310, whole genome shotgun sequence.
ACCESSION   DAOTGZ010000282 DAOTGZ010000000
VERSION     DAOTGZ010000282.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868088
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 3154)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3154)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:22:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,785
            CDSs (total)                      :: 2,751
            Genes (coding)                    :: 2,732
            CDSs (with protein)               :: 2,732
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 12 of 19
            Pseudo Genes (internal stop)      :: 5 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3154
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_36383_length_3154_cov_3.960310"
                     /isolate="AMR_MDS_3064"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..625
                     /locus_tag="P5123_12885"
     CDS             <1..625
                     /locus_tag="P5123_12885"
                     /inference="COORDINATES: protein motif:HMM:NF025616.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF4349 domain-containing protein"
                     /protein_id="HRX17204.1"
                     /translation="SSRQLLYDSIAKHGFLQNSRTSLYSTAPSLEINARIKSENFYQM
                     LKELRNAGSLKSEAINVYDLTSDNIMTLITKNRREERIKRRSSTLLNPSLSPKDHSAR
                     ERALEESEDQMDMAEHQLWQLQDRVTWASLTIYVTGPDTGKNIVVPRFKDAFTSLLNG
                     TLEVIYNLILVSPFLIILVIIFIKRKRLWDIFMTQHKSEKNREDYDD"
     gene            615..1106
                     /locus_tag="P5123_12890"
     CDS             615..1106
                     /locus_tag="P5123_12890"
                     /inference="COORDINATES: protein motif:HMM:NF014062.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NfeD family protein"
                     /protein_id="HRX17205.1"
                     /translation="MMIDFYLFQVSIPLVWLAAGIIISSTEFIIPTNISIWPGIAAAI
                     VALLIATGLVNEYSFVWQFFWFAIFTILLTLLWFFVIKKYTKLGSLTTDEHRDATLYN
                     LKGMVTETIRPGRPGKVELFSSFHGIKNWMAESDQTIEPETEISVLESDGIKLIVKKI
                     EGE"
     gene            1109..1990
                     /locus_tag="P5123_12895"
     CDS             1109..1990
                     /locus_tag="P5123_12895"
                     /inference="COORDINATES: protein motif:HMM:NF013324.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SPFH domain-containing protein"
                     /protein_id="HRX17206.1"
                     /translation="MNFYLVIALFVLAGVLILKGVRIVPQAENWIVERFGQYRVTLQA
                     GLNLINPFFSRVVKKIDIREQLIDMPSQSIITADNATVNVNGVVFFKIMDPYKAFYGI
                     DHLEFGIQSLAQTSLRAIMGKLTLDESLSKRDKINSELLAILDDATDAWGTKITRVEL
                     QDIDPPIDIKDAMASQMKAERNKRAQILEAEGQKQSAIEKAEGMKRSAILKAEGEKEA
                     AELEAEARERLAQAEAKALEMVAESLKVVKGDPMMYLLGQEYIKKMGDLAGSPNSKFV
                     VLPSDLQQTIKGLFDKK"
     gene            complement(2232..3065)
                     /locus_tag="P5123_12900"
     CDS             complement(2232..3065)
                     /locus_tag="P5123_12900"
                     /inference="COORDINATES: protein motif:HMM:NF012245.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein"
                     /protein_id="HRX17207.1"
                     /translation="MPYQKKAVKDIAVSADKFSEAAQAQATSTEEITATLEEISAEME
                     NVSDQTTDQLDSVNALFDRIKTLKENIVAVESRVSYTQKETDSILGKAESVKKLLQRM
                     ENKISRVGESSEKMVSIVAIIGNISEQINLLSLNAAIEAARAGNAGRGFAVVADEVSK
                     LAEQTASSLKDIYELINESNLHISEFTDESANVSEEIGIIGYGVININNLMNNVAKDL
                     KKEHKESELVVMDIEEVKVKADMIRNSTYEQKIAVADIVQTLYQKLMSYRKMVQPAPI
                     L"
BASE COUNT          962 a          625 c          600 g          967 t
ORIGIN      
        1 ctctagcagg cagctattgt atgactcaat tgcaaaacac ggctttttac aaaactccag
       61 aacttcacta tactcaactg ctccttcact cgaaattaac gcccgaataa aaagcgaaaa
      121 cttttatcaa atgctaaaag agctgcgaaa tgcaggctcg ctcaaaagcg aagcgataaa
      181 cgtatacgat ctgacttctg acaacataat gactcttatc acaaagaacc gccgtgaaga
      241 gagaataaaa cgccgctctt cgactctttt gaatccgtca ctctcgccta aagaccattc
      301 ggcacgagaa agagcacttg aagagagtga agaccagatg gacatggcgg aacatcaact
      361 ttggcagctg caagatcgtg tcacctgggc aagccttaca atttacgtta ccggccctga
      421 taccggcaaa aacattgttg tgcccagatt caaagacgca tttacatctt tgttaaacgg
      481 aacgcttgag gttatatata acctaatact tgtttcacca tttttgataa tccttgttat
      541 tatctttatc aaaagaaaga gactgtggga tatttttatg acacagcata aatccgaaaa
      601 aaacagagag gattatgatg attgactttt acctttttca ggtttctata cctctggtat
      661 ggctggcagc cggcattatt ataagttcga cagaatttat tattccaacc aatatttcga
      721 tatggcccgg aatagctgcc gcaattgtgg cattactaat tgctaccggc cttgttaacg
      781 aatacagctt tgtctggcaa tttttctggt tcgcgatctt tacaattttg ttgactctac
      841 tctggttttt tgttattaaa aagtatacaa aacttggaag ccttacaact gacgagcacc
      901 gtgacgcaac actatataac ctaaaaggca tggtaactga aacaattaga cctggacgcc
      961 cagggaaagt agagcttttt tcatcttttc atggaatcaa aaactggatg gctgaatccg
     1021 atcagactat cgagccggag accgagatat ctgtgcttga aagtgatggc attaagctta
     1081 tagttaaaaa aatagagggg gagtaaaaat gaacttttat cttgtaatag cactttttgt
     1141 tttagcagga gtgctgatac taaagggtgt acggattgta ccacaggctg aaaactggat
     1201 tgttgaacgt ttcgggcaat acagggtcac acttcaggct ggtcttaact tgataaatcc
     1261 atttttttca agggttgtaa aaaagatcga tattcgagag cagttaatcg atatgccaag
     1321 ccaatctatt attaccgcgg ataatgctac tgttaatgtt aacggtgtgg tttttttcaa
     1381 gatcatggat ccgtacaaag ccttttatgg aatcgaccat cttgaatttg gcattcaatc
     1441 gcttgcccag acatcattgc gcgccattat gggaaaattg acacttgatg aatcgctatc
     1501 aaaacgggac aaaattaaca gcgaacttct tgccatcctt gatgatgcca cagacgcatg
     1561 gggcaccaaa ataacacggg ttgaattaca ggatatagac cccccaattg atataaaaga
     1621 cgcaatggca agccagatga aggccgaacg taataagcgt gctcagattc ttgaggctga
     1681 aggccaaaaa cagagtgcga tcgaaaaagc ggaaggaatg aaacgttcgg caatattaaa
     1741 agccgaaggc gaaaaagagg ctgctgaatt agaagccgaa gcacgcgagc gacttgctca
     1801 agcagaagct aaggctctag agatggtagc cgaatcgtta aaggttgtta aaggtgatcc
     1861 gatgatgtat cttcttggcc aggagtacat taaaaagatg ggtgatcttg cgggatcccc
     1921 aaattccaag tttgttgtac taccgtcgga cttacagcag accataaaag gattatttga
     1981 taaaaaataa ctttttcggc gtacttaata gaaaatacag agcgataaag cgctctgtat
     2041 ttttttgcac actaagtaca aatataaatt atagagagag tagtttttaa aaattttgat
     2101 tacgataatt atattgtata caaaaccaaa ttcgggtttt gatcaattat cgtctttatt
     2161 tgtaatcttg aaatattttg tcttttcatg tatgttatcc gccatgctag ttaattcgac
     2221 ggtacttctg tttagagtat cggcgccggt tgcaccattt tgcgataact cattaacttc
     2281 tgatacagag tctgtacaat atccgctacc gctatctttt gttcataggt tgaatttcgt
     2341 atcatgtcgg cttttacctt gacttcttcg atatccatta caaccaattc actctctttg
     2401 tgttcctttt ttaaatcttt cgcaacgtta ttcataagat tattaatatt aattacacca
     2461 taaccgatta tacctatctc ttcagaaaca tttgccgact cgtcagtgaa ttcagaaata
     2521 tgcaaattag attcatttat taactcataa atatctttaa ggctggatgc ggtctgttcg
     2581 gcaagttttg aaacctcatc tgccacaact gcaaaaccac gccctgcgtt accggcacga
     2641 gcagcctcaa ttgcggcatt tagggataga agatttatct gttctgagat gtttccgatt
     2701 attgcaacaa tggaaaccat cttctcggaa ctttcaccaa ctctacttat cttgttttcc
     2761 attctttgaa gaagtttctt tactgactca gctttgccca atatcgaatc agtctctttt
     2821 tgcgtataag atactctaga ttcaacagct acgatatttt ctttaagtgt ttttatgcga
     2881 tcaaataatg cattaacact atctaattga tcagttgttt gatcagatac attttccatt
     2941 tcagccgaaa tttcttcaag ggttgctgta atctcttctg tagatgtagc ttgtgcttga
     3001 gcagcttctg aaaatttgtc agccgaaaca gcaatatctt ttactgcctt tttttgatac
     3061 ggcaacaaat tcttgaaata tttttaatcc attctcatct tcgtaatcac ttaaattttt
     3121 tccaatcaag tctttatcat aaggatgagc gaca
//
LOCUS       DAOTGZ010000283         3139 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_3064
            NODE_36604_length_3139_cov_4.440661, whole genome shotgun sequence.
ACCESSION   DAOTGZ010000283 DAOTGZ010000000
VERSION     DAOTGZ010000283.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868088
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 3139)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3139)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:22:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,785
            CDSs (total)                      :: 2,751
            Genes (coding)                    :: 2,732
            CDSs (with protein)               :: 2,732
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 12 of 19
            Pseudo Genes (internal stop)      :: 5 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3139
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_36604_length_3139_cov_4.440661"
                     /isolate="AMR_MDS_3064"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..439
                     /gene="glyA"
                     /locus_tag="P5123_12905"
     CDS             <1..439
                     /gene="glyA"
                     /locus_tag="P5123_12905"
                     /EC_number="2.1.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013011589.1"
                     /note="catalyzes the reaction of glycine with
                     5,10-methylenetetrahydrofolate to form L-serine and
                     tetrahydrofolate; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="serine hydroxymethyltransferase"
                     /protein_id="HRX17208.1"
                     /translation="LKEALDPAFKEYASQIIKNAAALANELAANGFNLVTGGTENHLM
                     LIDLRNKGVTGKQMAKAMDKAGIVCNYNSVPNDDRKPFDPSGIRIGTASVTTRGFQEE
                     EMKKIAGWMTKIALDHDNEELLLTIEKEVRALCDSLPVPKVFV"
     gene            477..1253
                     /locus_tag="P5123_12910"
     CDS             477..1253
                     /locus_tag="P5123_12910"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: protein motif:HMM:NF013509.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FKBP-type peptidyl-prolyl cis-trans isomerase"
                     /protein_id="HRX17209.1"
                     /translation="MKKITIILLFVLTLSSCDAIKEYFSSSKSKENEDNKKSDSSLDV
                     EDTLYLLGLDIGRSLQVFSLESDELELVLQGLKDYHTKDRKSLDEKLGKDRNNIRTLA
                     TQRKAVADAENLAKGKEYFDKYSKKSDVKTTESGLAYKIIEPGSSEKPLPTSTVTVNY
                     KGTLIDGTVFDSSYDRGKPATFALNRVIKGWGEGMQLIGKGGKIELVVPASLGYGVRG
                     AGSIPGNSLLIFEVELLDIDNTPKPVAKPKPSPVKIVPSK"
     gene            1603..2817
                     /locus_tag="P5123_12915"
     CDS             1603..2817
                     /locus_tag="P5123_12915"
                     /EC_number="2.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003028082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HRX17210.1"
                     /translation="MELKKSSKLDNVFYDIRGPVLEAADRMIREGHTIIKLNTGNPAA
                     FGITAPDEIVQDVKRNLMDAQPYGDSKGLYSARKAVMQDCQLKGIKDVDVDDIYIGNG
                     VSELIVMSMQGLLNNGDEVLVPIPDYPLWTGAVNLAGGTAVHYVCDEKSNWYPDIDDI
                     RSKVSNRTKAIIIINPNNPTGAVYPKELIEQIIAIAAENKLVVIADEIYDRILYDDAR
                     HYSAASLSDEVLFLTMNGLSKSHRICGFRTGWMIVSGNRTRAKDFIEGLEMLSSMRLC
                     SNMPSQYAIQTALGGYQSLNELISPGGRLYEQREYAHNIFNSIPGVSSVKPDGAIYLF
                     PKIDVKKFNISDDRKFVLDFLVQKKILLVQGTGFNWPDPDHFRVVFLPSVDDLKLVAD
                     RFADFLSTYRQD"
     gene            complement(2821..>3139)
                     /locus_tag="P5123_12920"
     CDS             complement(2821..>3139)
                     /locus_tag="P5123_12920"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="endonuclease/exonuclease/phosphatase family
                     protein"
                     /protein_id="HRX17211.1"
                     /translation="FNLIPPGQSYSLLPEDEKQYFQKETEMELLYNQFCVYPSLSDIQ
                     GENIYKWFTHFPNRQKAPDRIIDYIITSENIIPKDYFVRSNDTLDISDHLPLVMEFTI
                     KSQ"
BASE COUNT          974 a          524 c          695 g          946 t
ORIGIN      
        1 cttgaaagag gcgcttgatc ccgcttttaa agaatacgca tctcagatta ttaaaaatgc
       61 tgctgcgctt gctaatgagc ttgcagctaa cggttttaat cttgttacgg gtggcactga
      121 aaatcatctg atgctgattg atcttcgtaa caaaggggta accggtaaac agatggcaaa
      181 agccatggac aaggcgggta tcgtttgtaa ttataactct gttcccaatg atgaccgtaa
      241 gccgtttgat ccaagcggaa tacgaatagg tacagcttct gttaccacta ggggatttca
      301 agaagaagag atgaaaaaga tagctggctg gatgactaaa attgctttgg atcatgataa
      361 tgaagagttg cttttgacta tcgaaaaaga ggttagagct ctatgtgatt ctttaccggt
      421 tccaaaagtt tttgtataaa aataataaat agctgcatga ataaaaaaga gggaaaatga
      481 aaaaaatcac aataattttg ctgtttgttt taacattatc aagttgcgat gcaatcaagg
      541 agtacttttc ttcatctaaa tctaaagaga atgaagacaa taagaaatcc gattcatctc
      601 tggatgtaga ggataccctt tatcttttgg gactggatat tgggcggtcc cttcaggtgt
      661 tctctcttga gagtgatgaa ttggagcttg ttcttcaagg gcttaaggat tatcatacaa
      721 aagatagaaa gtcactggat gagaagctag gcaaagatcg caataatatt cgtactttgg
      781 caacgcagcg taaagccgtc gcagatgccg aaaaccttgc taaaggtaaa gagtattttg
      841 ataaatactc gaagaaaagt gatgtcaaga ccactgaaag cggtcttgcc tacaagataa
      901 tcgagccggg tagctctgaa aaaccgttgc ctacgagcac agttacagtc aattacaaag
      961 gaacacttat agacggaact gtttttgact caagttacga tagaggaaaa ccggctactt
     1021 ttgcgcttaa tcgagtaata aagggatggg gcgaaggaat gcagctgatc ggcaagggtg
     1081 gtaaaattga attggtagtt ccagcttctc ttggctacgg tgttcgtgga gcaggtagta
     1141 ttcctggtaa ctctcttctt atttttgaag tggaacttct ggatatagat aataccccga
     1201 aaccggttgc aaaaccaaaa ccttcacccg tcaaaattgt tccgtcaaaa tagggtaaag
     1261 tgatatattg gtttggatag tttataggtg ggtttaaccg atatatatag caactcatat
     1321 gattgtttat tagaaaccgt tattgataga aatgggatca gcaagtgctg atcccgattt
     1381 tattaatagg aggtttgttg tgttcaataa aacttcagcc gttgctagct aaaatatttg
     1441 tgcatataaa aagaaaaaag ttacaaaaaa acgggtttag tcataaacaa ataaaaaatt
     1501 attaaatttg tgattgcaaa aagagtgacg actggatttt gtaattagcg ctttgaaatt
     1561 acatactccg taatgaagat tattactacg cgtggagatc caatggaact aaaaaaatca
     1621 agcaagctgg ataatgtatt ttatgatatt agaggccctg ttctagaagc cgccgatcgt
     1681 atgatcaggg aggggcacac aataataaaa ttaaatacag gtaatcctgc tgcttttggt
     1741 ataactgcac ctgatgaaat tgttcaggat gtaaaacgta atcttatgga tgcccaaccc
     1801 tatggggatt caaaagggct ctattcggct cgaaaggctg taatgcagga ttgccagctt
     1861 aagggaatta aggatgtaga tgtagatgat atttatatcg gtaatggtgt cagtgaactt
     1921 atcgttatgt caatgcaggg actcttgaat aatggtgacg aggttcttgt ccctataccc
     1981 gactatccgc tatggacagg agctgtaaat ctggccggtg gtaccgctgt acactatgtt
     2041 tgtgatgaaa aatcaaattg gtatcccgat atcgatgata ttcgatctaa agtatcaaac
     2101 cgcacaaaag ctataattat tataaatcca aataacccaa caggagccgt ttatccaaaa
     2161 gaactgatcg aacagattat tgcaatagca gcagagaata aacttgttgt gattgcagat
     2221 gaaatatacg atagaatact ttacgatgat gctcgtcact attctgctgc atcactttcc
     2281 gatgaggttc tttttcttac catgaatgga ctttcaaagt cacaccgtat ttgtggcttc
     2341 cgtactggct ggatgatcgt tagtggaaat cgtactcgtg ctaaagattt tattgaaggg
     2401 cttgaaatgc tctcctccat gcgtttatgc agtaatatgc cttcgcagta cgctatccag
     2461 acagcacttg gagggtatca gagccttaac gaactgatct ctccgggggg gcgcctttat
     2521 gaacagcgtg aatatgctca taatattttt aattccattc ctggtgtatc ttcggtaaag
     2581 cctgatggag caatttatct ttttccaaaa attgatgtaa aaaaatttaa tattagtgat
     2641 gaccggaagt ttgttttgga ctttcttgtg caaaaaaaga tactgcttgt acagggaacc
     2701 ggttttaatt ggcctgatcc ggatcatttt agggttgtat ttttaccttc ggttgacgat
     2761 ttgaagttag ttgccgatag gttcgccgat tttttatcga cttatcgtca ggactagaat
     2821 ttactgggat ttaatggtaa attccataac tagcggaagg tgatcagaaa tatccagtgt
     2881 atcgttcgat cgtacaaaat agtcttttgg tattatattt tctgatgtaa ttatatagtc
     2941 aataatgcgg tcgggcgcct tctgtctgtt tggaaaatgt gtaaaccatt tgtatatatt
     3001 ctcgccttga atatcactta aagagggata aacacaaaac tggttataga ggagttccat
     3061 ctctgtctct ttctgaaaat actgtttctc gtcttccggt aaaaggctgt aagactgtcc
     3121 ggggggtatc agattaaag
//
LOCUS       DAOTGZ010000284         3120 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_3064
            NODE_36879_length_3120_cov_4.778793, whole genome shotgun sequence.
ACCESSION   DAOTGZ010000284 DAOTGZ010000000
VERSION     DAOTGZ010000284.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868088
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 3120)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3120)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:22:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,785
            CDSs (total)                      :: 2,751
            Genes (coding)                    :: 2,732
            CDSs (with protein)               :: 2,732
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 12 of 19
            Pseudo Genes (internal stop)      :: 5 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3120
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_36879_length_3120_cov_4.778793"
                     /isolate="AMR_MDS_3064"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..529
                     /locus_tag="P5123_12925"
     CDS             <1..529
                     /locus_tag="P5123_12925"
                     /inference="COORDINATES: protein motif:HMM:NF013703.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015297 - antiporter activity [Evidence
                     IEA];
                     GO_function: GO:0042910 - xenobiotic transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="MATE family efflux transporter"
                     /protein_id="HRX17212.1"
                     /translation="IGVLVGQNIGAGKIERAQAVAKLGQWLSFITLTVCGLLAFTFAH
                     HIARFFVPGDADVIREGARFIQIMSFSFGLIGVQMAVNGILRAAGNMMAPMMMTIVSQ
                     WVVLFPLAYILSKHTDFGITGLWYAFPIANVIMVVIALVWFNKIPWQTKNLTKEDHEE
                     IRATQEILIEEGVRG"
     gene            913..2040
                     /locus_tag="P5123_12930"
     CDS             913..2040
                     /locus_tag="P5123_12930"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRX17213.1"
                     /translation="MNGLTSNQRQQLFGSVYNGNDDEILKSVLGIESLPSPYKTSLIP
                     MYERIPDQDMKKYLEKEFSFFVSQGCKFACKFCAAIRTQKDALGTVTKVTEVYRDFDI
                     MEQDLSYLLGRARTLGVSHIDIYMSNLDVFQSPVQLGKFADTVIKVRSNIPEIQVTLR
                     ALATIDSFLNCAEKHPDVITKLVQAGYTTTGFGIDGGAPEVWRKVGKAHNFSSKDSDP
                     AKKSIEALRISKEVFGITPEILMVFGHPDVDTVDSMQSAVDFTHAMVDLYGAVPRPHI
                     AKNLIPGNDHWMNPANQAEVQMFIQEPWLFQALDFCALATEYTHGNKDFAKLVNKYYL
                     AICNIEGNTTQPIYPIELTNKQVTLSGIQNMLAVNLNRFDR"
     gene            2157..2633
                     /locus_tag="P5123_12935"
     CDS             2157..2633
                     /locus_tag="P5123_12935"
                     /inference="COORDINATES: protein motif:HMM:NF013375.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate kinase"
                     /protein_id="HRX17214.1"
                     /translation="MIIFVWGLPGAGKSTVGRMLAEKLNLGFYEMDDYLPDSFKEKMK
                     SGKLITDEDRDSFFSTFTKKLKEIDTGKDFVVSGYVAKKRHRDQLKNIGDLRFFELVA
                     DRALLTERLKKRKDHFFEEETLKKVLKNYEPLNDSVKIDAEKSIAYVVKQIIDSLN"
     gene            complement(2761..3087)
                     /locus_tag="P5123_12940"
     CDS             complement(2761..3087)
                     /locus_tag="P5123_12940"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRX17215.1"
                     /translation="MPENTQNTIRFENKDVSVLGKAKDYTDKKISTINLFMSVVLVVL
                     VLGFIQLIVDSFRFSSVTYKEYSEKTEMVESINNINKELLEQNKSNQQLIIDQQENIL
                     RLIKNK"
BASE COUNT          962 a          532 c          628 g          998 t
ORIGIN      
        1 gattggagta ctggttgggc aaaacattgg tgcaggaaag attgaacgtg cacaggctgt
       61 tgctaaacta ggtcaatggt tatcgttcat cacgctcacc gtatgtgggc ttctcgcgtt
      121 tacgtttgcg caccatattg cgcgattctt tgtgccaggt gacgcagacg ttattcgcga
      181 gggagcgcga tttattcaaa ttatgtcgtt ctcatttggc cttatcggag tacagatggc
      241 agtcaacggc attctacgtg cagcagggaa tatgatggca cccatgatga tgaccatcgt
      301 atcgcagtgg gtcgtattat tcccattagc gtatattctc tcgaaacata ctgattttgg
      361 tattacgggt ctttggtacg catttcctat tgcgaacgtg attatggtgg tgattgcact
      421 cgtgtggttt aacaagattc cgtggcaaac aaaaaatttg accaaagagg atcatgaaga
      481 aattcgtgca acacaagaaa ttttaattga agagggggtg agaggataga attttttgaa
      541 gttatgcttt ttattcgtga ataaaatccc gtaagtgtat attgacatat tattaaatag
      601 ttaatatagt ataagaaact caaactttac aatactatga aaaacaaaat ctttgaccta
      661 ttacagcccc ggcatattta tgctggatca acgggtcttg gccatgttta catgccaaca
      721 tcgttattga ccacgggatc tcggctgtta caagcaggtt atgaggtttc tttgttcgat
      781 gaaaatatac gaccttatag aggtactgct caaaacttgg gtataaacct cttgggatca
      841 ccctatgttc ccgtagtaaa ggatcgatta catacattgt ctcagcaaaa catttttttg
      901 ggaggacagg tcgtgaatgg actaacttcc aatcaaaggc aacaactttt cggttcggtt
      961 tacaatggta atgatgatga aatcttgaag agcgtcttag gtatcgaaag tctgccatcg
     1021 ccctacaaaa catcattaat tcccatgtat gaacgcattc ctgaccaaga catgaaaaag
     1081 tatcttgaaa aagaattttc cttctttgtc tcacaggggt gcaagtttgc ttgtaagttt
     1141 tgcgcggcta ttcgtaccca aaaggatgct ttgggcacgg ttacgaaagt taccgaagtg
     1201 tatcgggatt ttgatattat ggagcaagac ctatcctatt tacttggtcg tgcacgtaca
     1261 ttaggagtgt ctcatattga tatttacatg tcgaatttag atgttttcca atcacctgta
     1321 caattaggaa aatttgccga tacagttatc aaagttcgta gtaatattcc tgagattcag
     1381 gtaactctgc gagcgttggc taccattgat tcttttttga attgtgctga aaagcatcca
     1441 gatgtaatta cgaaacttgt gcaagctggt tataccacta caggttttgg aattgatgga
     1501 ggagcacctg aagtttggcg aaaggttggt aaggcgcata acttttcatc caaagatagt
     1561 gatcctgcaa aaaagtccat tgaagcgctt cggatatcaa aagaagtttt cggtattaca
     1621 ccagagatat taatggtatt tggtcatcca gatgttgata cagtcgattc tatgcaatct
     1681 gctgttgact ttactcacgc gatggttgat ttatacggag ccgttccacg acctcatatt
     1741 gcaaaaaacc tgatccctgg gaatgaccat tggatgaacc cagcaaacca agcagaagta
     1801 cagatgttta tacaagaacc ttggcttttc caagcattag atttttgtgc attagctact
     1861 gaatatactc atgggaataa agattttgcg aagttggtta acaaatatta tttagcaata
     1921 tgtaatattg aaggcaatac aacacaacct atttatccaa tagagttgac aaacaaacag
     1981 gtaacactat cgggcattca aaatatgctt gccgtgaact tgaataggtt cgataggtag
     2041 tttgtctgtt tcagtattaa aaaaccaagg aatattaccc cttggttttt ttatactcat
     2101 tagccaacct acgtagtacg tctttttaac ctaactttgt tataatgaat ggtatcatga
     2161 ttatatttgt ttgggggctg cctggtgcgg gcaaatcgac agtaggaaga atgcttgcag
     2221 aaaaactgaa tcttggtttt tatgaaatgg atgattattt accggatagt tttaaggaaa
     2281 agatgaaatc aggtaagtta ataactgatg aagatagaga ctcttttttc tccactttca
     2341 cgaaaaaact taaagaaata gataccggca aagattttgt agtttctggg tacgttgcaa
     2401 aaaaaagaca tagagatcaa ttaaaaaata ttggcgatct gagattcttt gaattggttg
     2461 cagaccgagc tctcttaacc gaaagactta aaaaaagaaa agatcatttt tttgaggagg
     2521 agaccttgaa gaaagtttta aaaaattacg aacccttgaa cgactcggta aaaattgatg
     2581 ctgagaaaag tattgcctat gtagtgaaac agatcataga cagtcttaac taaaagcccg
     2641 tgtaggcatt aagctagctt tttctgtgag ccttctcgag ctagaactaa ataaatacat
     2701 tacggggtaa agctttttaa ggtattaaaa taatattact aacgatccct ctcgagcatt
     2761 ttatttattt ttgattaaac gaagtatgtt ttcctgctga tcgattatta gctgctgatt
     2821 acttttgttc tgttctaata attctttatt tatgttattt attgactcta ccatttctgt
     2881 cttttctgaa tattctttat acgttacaga actaaaccta aatgaatcta caatcaattg
     2941 aataaaacca agaaccagaa caaccagaac aactgacata aaaagattta ttgttgatat
     3001 ttttttatca gtataatctt ttgctttccc taaaacagag acgtctttat tttcaaacct
     3061 tattgtattt tgtgtgtttt ctggcatggc tatatttcaa tattaggtag taaaacctta
//
LOCUS       DAOTGZ010000285         3103 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_3064
            NODE_37162_length_3103_cov_7.065945, whole genome shotgun sequence.
ACCESSION   DAOTGZ010000285 DAOTGZ010000000
VERSION     DAOTGZ010000285.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868088
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 3103)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3103)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:22:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,785
            CDSs (total)                      :: 2,751
            Genes (coding)                    :: 2,732
            CDSs (with protein)               :: 2,732
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 12 of 19
            Pseudo Genes (internal stop)      :: 5 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3103
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_37162_length_3103_cov_7.065945"
                     /isolate="AMR_MDS_3064"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            299..778
                     /locus_tag="P5123_12945"
     CDS             299..778
                     /locus_tag="P5123_12945"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRX17216.1"
                     /translation="MEATDFRTSIDVEVNRLEKIIDYAEKHNMDISDVINTVLDKYSK
                     KLAKNPVCNDSLNKYQERGTIYKSVHIRFSSITKRKLKDIANLYCYSLSYMLFLAMDF
                     FEEFMDGVKEFIRPCFHASFVRLFEFPCFINSWGINFYNSPDYSSGQETSKLFHLLS"
     gene            complement(840..1385)
                     /locus_tag="P5123_12950"
     CDS             complement(840..1385)
                     /locus_tag="P5123_12950"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRX17217.1"
                     /translation="MIVIINGSVGSGKSTAAYSLQQQFTRSVMLDGDCIGAVHPFQIY
                     DDSRIEYLYDTLLYLIKFHKSNGYQDFVINYVFETSQSLQSLVNRLETVDPKVCCYRI
                     TCSAEEQKKRIINRNSDQVDWELQRFIELNAIQEKAAKTGFIGKKFVTDGLSPSQIAE
                     RIIMDIKENSCREESELLVGS"
     gene            complement(1532..2932)
                     /locus_tag="P5123_12955"
     CDS             complement(1532..2932)
                     /locus_tag="P5123_12955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020260449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidophosphoribosyltransferase"
                     /protein_id="HRX17218.1"
                     /translation="MGGFFGVTSHGDCVADLFYGTDYHSHLGTRRGGMAVCNDKDFMR
                     YIHDISNAQFRSKFELDVLKLTGRSGIGVISDYEDQPLLISSHLGNYAIVTVGAIKNI
                     KELVKKAFANRVTHFSEMVGGEINPTELVATIINQGSTIKEGLKLVQESIEGSCSILL
                     LTTEGIYASRDKYGRTPIVIGKKNGSYAVAMDTCAFPNLEYEIEHYLGPGEITLVKPE
                     GYQTVAPAGDKLKICTFLWIYYGYPASSYEGINSEVVRNRNGRLLAKNDDVVIDSVSG
                     IPDSGVGHALGYAHEAKVPYTRSFVKYTPTWSRSFMPQHQKKRDLIASMKLIPVRELI
                     DGKKLLYCDDSVVRGTQLRDIISRLYNCGAKEIHMRLACPPLVYTCKYLNFSRSRNEM
                     DLAARREISKLEGGVCKNLEAYTDSSSECYKKMVNNIREGLGLTTLKYQDLKSMLDAV
                     GLPHENLCTYCWDGKE"
BASE COUNT          982 a          664 c          528 g          929 t
ORIGIN      
        1 aatccgtcaa aacaatcgcg cttgaggatt acgcaattgt tttgactatg tggttctttc
       61 tttttgccat aaaaaaaaag gacaacaacc ttgttttgtt aaaaacaaga aaacccctct
      121 gaacgagaaa aaagaaaaaa aagaaataac aaaaagattg ttgctctaca aatatcaact
      181 ccacggattt aatgagtaac tgttaaattt cttgccaaat gaaaaaaatg gctaaaaaat
      241 tttaacaaaa cagctttcgg tccagtatat ataaatagaa acaacatgga ggcataaaat
      301 ggaagctacc gattttagaa catcaattga cgtggaagtc aacagactgg aaaaaatcat
      361 agattacgca gaaaaacaca atatggatat ttcagatgta atcaatacag ttcttgataa
      421 gtattcaaaa aagctggcta agaatccagt atgcaatgac tctcttaata aatatcagga
      481 gagaggcacg atttacaaat cagtgcatat acgattttca agtattacaa aaagaaaact
      541 gaaggatatt gccaatcttt actgttacag cctgtcatac atgttatttt tggcaatgga
      601 cttcttcgag gagtttatgg atggtgttaa agagtttatt cgtccctgtt ttcatgcttc
      661 ttttgttcgt ttgttcgaat ttccatgctt cataaacagc tggggtataa acttctacaa
      721 ttcccctgac tattcttctg gacaagaaac cagcaaattg tttcatttat taagttgatc
      781 tatattattt agatgatttg ttcgacaaca aaactcctct ctggataaaa agaacatatt
      841 caactgccaa caagtaattc gctctcttcg cggcagctgt tttccttaat atccattata
      901 atgcgttcgg caatctggct gggagacaga ccatctgtta caaatttctt tcctataaag
      961 cccgtcttcg ctgccttttc ctgaattgca ttgagctcga taaacctttg aagttcccag
     1021 tcaacctggt cactattacg gttaattata cgtttttttt gctcctcagc cgaacaggta
     1081 atccggtagc agcaaacctt cggatcaact gtctccagtc gattaacaag actctgtaaa
     1141 gattgcgagg tctcgaaaac gtaatttatc acaaaatcct gatatccgtt ggatttgtga
     1201 aatttgataa gatataataa ggtatcgtac aaatattcga ttctggagtc atcatatatc
     1261 tggaaagggt gaacagcacc aatacaatcg ccgtccaaca ttaccgatct ggtaaattgt
     1321 tgctgtaaag aataagctgc agtcgatttg cctgatccga cagatccgtt aattatcact
     1381 atcattctga ttatcccgag tttttaagtt aaaaaccagt ttctgataat ttgtttgtaa
     1441 aatcaactgt tttgtacatt tatgtaagtt ttaaggctat aaagctggct aacaagccgg
     1501 caaaaactcc ctttactgcc tgaaaaatct gttactcttt gccatcccag cagtaggtac
     1561 aaagattctc atgaggcaag ccaacagcat caagcatcga tttcaaatct tgatacttga
     1621 gtgttgttaa tcccaaacct tcacgaatgt tgtttaccat cttcttataa cattccgatg
     1681 aactatcggt ataggcttca aggtttttgc aaactccacc ctcaagcttg cttatttctc
     1741 gacgagcagc aagatccatc tcatttcgcg agcgagaaaa atttaggtac ttacaggtat
     1801 aaacaagtgg tggacacgca agtcgcatat gaatctcttt agctccacaa ttataaagac
     1861 ggcttattat atcccgaagc tgtgtacccc ttacaaccga atcatcacag taaagaagtt
     1921 ttttgccatc aatcaattct ctaaccggta tcaacttcat gcttgctatc aaatcacgtt
     1981 tcttctgatg ctgcggcata aaacttcgag accaggttgg tgtatactta acaaacgatc
     2041 tggtataggg aactttggcc tcatgagcat atccaagcgc atgtccaact cccgaatccg
     2101 gaattccgct aacgctatca ataacaacat catcattttt tgccaaaagc cttccatttc
     2161 tgtttcgcac aacttcagaa ttaattcctt cataacttga agcggggtag ccataatata
     2221 tccacaaaaa agtacaaatc ttaagtttgt ctcctgcggg agcaacagtt tgataacctt
     2281 ccggtttaac cagcgttatt tcacccggcc ctaaatagtg ttcaatctca tattccagat
     2341 ttggaaaagc acaagtatcc atggcaacag catagctacc attttttttt ccaataacaa
     2401 ttggcgtgcg tccgtatttg tcccttgaag cgtatatacc ttcagttgta agaagcagta
     2461 ttgagcatga cccttctata ctctcttgta caagtttcaa cccctcttta attgtcgagc
     2521 cctgattaat aattgtcgca acaagttcgg tcgggtttat ctcaccacct accatttccg
     2581 aaaagtgtgt tacccggtta gcaaaggctt tttttaccag ttccttgata ttttttatgg
     2641 caccaacggt tacaatagcg tagtttccaa gatgagaaga tattagaaga ggctgatctt
     2701 cgtaatcact gataaccccg attccgctgc gacctgtgag tttaagaaca tccagctcga
     2761 attttgatct aaactgagcg ttagatatat catgaatata tctcataaag tctttatcgt
     2821 tacaaacagc catgccgccc cgacgtgtac ccaaatgaga atgataatcc gtgccataaa
     2881 aaagatcggc aacacaatct ccatgagacg tcactccaaa aaaaccaccc atatcattag
     2941 ccccaatgca taaattactt agtatcacag gaagagttgt cacagcctta aacaacgcgg
     3001 attttgccaa taacaaaata cttaaacatg atttgctccc gagaggattc gaacctccgg
     3061 cacatagttt aggaaactac tgctctatcc acctgagcta cgg
//
LOCUS       DAOTGZ010000286         3074 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_3064
            NODE_37624_length_3074_cov_7.120570, whole genome shotgun sequence.
ACCESSION   DAOTGZ010000286 DAOTGZ010000000
VERSION     DAOTGZ010000286.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868088
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 3074)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3074)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:22:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,785
            CDSs (total)                      :: 2,751
            Genes (coding)                    :: 2,732
            CDSs (with protein)               :: 2,732
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 12 of 19
            Pseudo Genes (internal stop)      :: 5 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3074
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_37624_length_3074_cov_7.120570"
                     /isolate="AMR_MDS_3064"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(440..778)
                     /locus_tag="P5123_12960"
     CDS             complement(440..778)
                     /locus_tag="P5123_12960"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRX17219.1"
                     /translation="MALLEMSELLSDSVLVVFVPTGLCQSCLSSLFLCLRETEWPMNN
                     VRILSTSDDFLIKKVINSIGCQYFLSNNDYQESTSIIILRKVKGYNPIYIKYSDEYEF
                     ILKLFLAQEI"
     gene            complement(951..2120)
                     /locus_tag="P5123_12965"
     CDS             complement(951..2120)
                     /locus_tag="P5123_12965"
                     /inference="COORDINATES: protein motif:HMM:NF028479.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-bladed beta-propeller"
                     /protein_id="HRX17220.1"
                     /translation="MKNFRSIYVLLLLILLVTSCWSDRNEEETSVYESILPLDSTVEI
                     DGLSQYVDSLEIIPIINNAGVSLNIISKMLVTENNFIVLASGSVFSLTKDGKTVKQYG
                     ESGRGPGEYHSVFDICLDNSEEEVWCLCYSFPPKIQKYDLVSHILIEEIEIEPLIDAT
                     GIIPLKNGGFMLYVPNPDYNDLANSEKDFYCLKIFDRNGLYKGEKLKQKDFNLNAAFF
                     RPVSQSSGNRYVLAPGSSSESCLIYENGELMKRINFDFGDKTLPAGFAFQGVNNPWEN
                     IGVIFSLDYYKYVSSIYFLDNGVFFTAHGKESSVWHFFKGGSSGIRWRSIGKLTPPMR
                     AVGADDTYVYFICYDYGEIPLKEELDPLKRYVIQTKRFPCENEVSIIIKVKFRVE"
     gene            complement(2132..2341)
                     /locus_tag="P5123_12970"
     CDS             complement(2132..2341)
                     /locus_tag="P5123_12970"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRX17221.1"
                     /translation="MDVGGSGGGGYTTGCPGVQNRRKSQAVQCAGGGWVIKPGCCSGY
                     MRCDAMNPCNGQQFICHGGLCDPEL"
BASE COUNT         1014 a          624 c          498 g          938 t
ORIGIN      
        1 cagacttagg atctggtgcc gaaaggcgtg ggggttcgag tccctttacc cgcaccagca
       61 aatcaaccct gcagtttttc tgcagggttt ttatttgtca cgattgataa gggaaacctc
      121 gtcccacgga gggaggtata taatactgtc gtaaacagcg ctatcatcaa cttaatttct
      181 accattcatg aatcgagtat tttaaatact atattttcaa tgcaattata ttaggactca
      241 aatcattgaa atgcctcgtt tcaattttct ggcccataga tattgtctct tcaacatctt
      301 catatcctta aattatatat gaagtaacac aggacatacc tatgaaaacc ccgtaaattg
      361 caggttatca aaggttttga ggttataaaa taaaatctct atatgtacag tttattcgta
      421 taagagctaa aaaacggagt taaatttctt gtgccagaaa aagcttcaat ataaattcat
      481 actcgtcact atattttatg taaatcggat tatatccctt gacttttcgt aatataatta
      541 tacttgttga ttcctgataa tcgttattgg acaaaaagta ttggcaacca atagaattaa
      601 ttactttctt aattaaaaaa tcatcagaag tgcttaagat cctcacattg ttcattggcc
      661 attccgtttc acgtaagcat agaaataaag aagaaagaca tgactggcat aatcccgttg
      721 gaacaaacac tacaagcacg ctgtcgctta ataactctga catttctagc aaggccatat
      781 tctcctcatc acgacttatc aaagagctag agttgagaat ataattactc aaaatatgtt
      841 ctctctgtat aaatacactt ttcaaatcag acgttttctt ttgttgtaac actattaaag
      901 taaacaacaa aagagtaagt gaaacaagag taaaaataac caatgtattt ttattcaaca
      961 cggaatttca cttttataat aattgagact tcgttttcac aggggaatcg ttttgtctga
     1021 attacatatc gttttaatgg atccaactct tccttaagcg gtatttctcc gtagtcatag
     1081 caaataaagt aaacatatgt gtcatccgct cccactgccc gcattggagg cgttaattta
     1141 cctattgatc tccaacgtat gccagatgat cctcctttaa agaaatgcca tactgacgat
     1201 tctttaccat gtgcagtaaa aaatactcca ttatccagaa aataaataga tgacacatac
     1261 ttataataat caaggctgaa aatcactcca atattttccc atggattatt gactccttgg
     1321 aatgcaaaac cagcaggtaa tgttttatct ccaaaatcaa aattgattct tttcatcaat
     1381 tctccatttt catatataag acaggattca cttgaggatc cgggagcgag aacatatctg
     1441 tttccagatg actgtgaaac aggtctgaaa aaagccgcgt ttaagttaaa atctttctgt
     1501 ttcaatttct ctcccttgta aagaccattc cgatcgaaaa ttttcaagca ataaaaatcc
     1561 ttttcggaat ttgcaagatc gttataatca ggattcggta catataacat aaaaccgcca
     1621 ttttttaacg ggatgattcc ggtcgcatca attaatggct cgatttcaat ctcttctatt
     1681 aaaatatgac tcactaaatc atatttttga attttgggtg gaaatgaata acaaagacac
     1741 cagacttcct cttccgagtt gtcgagacat atgtcgaaaa cggaatgata ttctccaggt
     1801 cctcgaccgc tttctccata ttgcttgaca gttttgccgt ccttagtcaa agaaaaaaca
     1861 gaaccgcttg ccaaaacaat gaaattgttt tccgttacga gcatcttcga tattatattc
     1921 aaactaacac cagcattatt aataatgggt attatctcca gagaatcaac atattgagaa
     1981 aggccatcaa tctcaacagt ggagtcgaga ggcaagatag actcgtaaac actagtttcc
     2041 tcttcattac gatctgacca acagctagta acaagtaaga tcagcagtaa aagaacataa
     2101 attgaacgaa aatttttcat gttattcaag attacaattc agggtcacac aaaccaccat
     2161 gacaaataaa ctgttgacca ttacatggat tcatagcgtc acaacgcata taacccgagc
     2221 aacagccagg ttttattacc catccgcccc cagcacattg tacggcttga gacttgcgac
     2281 ggttttgcac tccagggcaa cctgttgtat acccaccacc accactacca cctacgtcca
     2341 tatactccgg gacgtcttgg cacaacgcgg tgattgatat gcttgtctgt aagacactca
     2401 ctgttaaaaa aacagaaaga ctaataacta caagaaaaaa aaaccaaaaa ttaatttttt
     2461 cataattgag aacgtccggc tccggccccg atgagccaaa taatgattta ataaaagtgt
     2521 gaggccttac gcagtaaaac gagcttaaat atcagaggtt taaatcacta gaggagcgaa
     2581 aacactccgg caatcaaaca tagaaattat ttttgataaa tccaaataac cgagtacttt
     2641 ctttattaaa attatcgatc gtgttgcgct ctaagcaacc ttttggcagt gctgcttctc
     2701 aaatcacaaa aggttttagg ttttatagta ggctaagtcg ctttttggtt catatcatgc
     2761 tctgtattcc tgttgtaaca tgaatttata cctgaataac atccaaatcc aattttagtt
     2821 caccatgaaa gtctcaatga agcattgtac ctgctccagt gaggctaacc tttgaatgct
     2881 gaatacttcc tccttctcac aaaaaataaa tgttacaggc acggaaactt ttacaggcat
     2941 cttataatac tgctgtatat tcggttcttc gtcaattttg gtgcaggaga aggtgttcgc
     3001 aaaatggcta aaaaattagc aagaaattga gggtcctgca tttttgcctc catattcgcg
     3061 aggtattcct tttt
//
LOCUS       DAOTGZ010000287         3072 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_3064
            NODE_37659_length_3072_cov_4.658601, whole genome shotgun sequence.
ACCESSION   DAOTGZ010000287 DAOTGZ010000000
VERSION     DAOTGZ010000287.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868088
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 3072)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3072)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:22:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,785
            CDSs (total)                      :: 2,751
            Genes (coding)                    :: 2,732
            CDSs (with protein)               :: 2,732
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 12 of 19
            Pseudo Genes (internal stop)      :: 5 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3072
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_37659_length_3072_cov_4.658601"
                     /isolate="AMR_MDS_3064"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..2778
                     /locus_tag="P5123_12975"
     CDS             <1..2778
                     /locus_tag="P5123_12975"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HRX17222.1"
                     /translation="TIRLYTHDKRLLFYYSAVVPKKTVTGLHIKTITGNDTFVDPDLF
                     SITKEISDDFHIPYYPAPNTIVPYFKSTIPTKRESRLFINNNNVIIRVIEPFSLLKGK
                     NGFIIAEYLISDIDTSLYKRFTRTDINFFTNDSLSCGTLKNAPIPLNDKAYDKIINSE
                     SGLSRSAKKFQLSEVRINKIKYYQGHTRFYVNKNKFGTITASLKKHTELMEILTTVIL
                     VVVSAMTVILITFGLSIFFTKPYLQFIHNMINSVSAVAAGNLDQNIPTHTRDELGILA
                     VSFKDMKDSIQQIISEQKRLVTILETTSDMICMYRSESEIMYMNSSLKRVLSDKDITK
                     FNLYNLYPEWARDLLEHEGYFEAIRTGIWAGESAIIDESGVEIPVSQVIVCHKNNDSV
                     IEYFSTIMRDISDRKKNENLIKQNEENLSITLNSIGDAVIVTDNNGFLVRMNPIAEEL
                     TGWENSETTGKPLDDIFRIVNYLDKTPLPNPVLQVIQNKQIINLQPNALLISKDNSEY
                     RISDSAAPIIRDDGSLLGVVLVFRDETEKIKKDMQLIQSQKMEAVGTLAGGLAHDFNN
                     VLGGIIGTASLLKKQLSNNDTIDKNKLLDHISLIEQSGEQAADIVRQLLTISHNQEFT
                     FETVDLNQSVKSVSEMAKNTFDRSISIKITYLDKPALINADRNQIEQVLLNLFINASH
                     AMTIMRPDADIWGGTLSVDIREISTSDTSGNRIRKIRLDVQDSGTGIRKVDLKKIFTP
                     FFSTKGKGKGSGLGLSMVYSIIEKHGGEISVYSEINRGTTFTLLFPLIESKTTENKKV
                     PKKNIIERGSGLILVIDDEEILRKTSKAMLEECGYEVLTAKSGNEGIALYNENKDRIS
                     GIILDMIMPGLSGKETYQKLKEINPDVKVILASGFKQDERIESAMKMGIRDFIQKPYS
                     LSELSLKAKSF"
     gene            complement(2850..>3072)
                     /locus_tag="P5123_12980"
     CDS             complement(2850..>3072)
                     /locus_tag="P5123_12980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002644741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="MarC family protein"
                     /protein_id="HRX17223.1"
                     /translation="LVIGSLAVLAATALCIATIYILKRVHDYVRTRREALIQRYIEIA
                     GRITALFVGTVAIEMIFQGLKSWIAKMII"
BASE COUNT         1108 a          565 c          539 g          860 t
ORIGIN      
        1 actataagac tttacacaca tgacaagcgt ctgctatttt attattcagc ggtggttccg
       61 aaaaaaaccg ttacggggct tcatataaaa accattactg gtaatgatac ctttgtcgat
      121 ccagatctct tttccattac aaaagaaatt tcagatgatt ttcatattcc atattatcca
      181 gcaccaaata ctatcgttcc ctattttaag agcacaattc ccactaaaag agaatccaga
      241 ctttttatca ataataacaa cgtaataata agagtaattg aacctttttc tcttttaaaa
      301 ggtaaaaacg gctttattat cgcagaatac ctgatatcgg atatagatac aagtctatac
      361 aaaagattta ccagaacaga tattaacttt tttacaaatg atagccttag ttgcggaacc
      421 cttaagaatg cgcccattcc attaaacgat aaagcttatg acaaaataat caactcagaa
      481 tccggtttat ccagatcagc aaaaaaattt caactttcgg aggtacgaat caacaaaata
      541 aaatattatc agggtcatac ccgcttttac gtaaataaaa acaaattcgg aacaataaca
      601 gctagcttaa aaaaacatac tgaattaatg gaaattttaa ctacggttat tttggttgtg
      661 gtatcggcga tgactgttat actgataacg tttggtttat caattttttt cacaaaaccc
      721 tacctgcaat ttattcataa tatgattaat tctgtttcgg ccgttgctgc aggaaatttg
      781 gatcagaata tccccactca cacaagagac gagctcggaa ttcttgcggt atctttcaaa
      841 gatatgaaag attcgataca acaaataatc agtgaacaaa aaaggttagt aaccattcta
      901 gaaaccacaa gcgatatgat atgcatgtat cgcagtgaat ccgaaattat gtatatgaat
      961 agctcactaa aaagggtatt atcagataaa gatataacca agtttaatct gtacaatctt
     1021 tatcctgaat gggctcgtga tctgcttgaa cacgaagggt attttgaagc tataagaacc
     1081 gggatatggg ctggagagtc ggcaataatt gacgaaagcg gcgtagaaat ccccgtatct
     1141 caagtcattg tttgccacaa aaacaacgac tcggttattg aatacttttc tactattatg
     1201 cgggatattt ctgatcgtaa aaaaaatgaa aacctgatca agcaaaatga agaaaatctt
     1261 agtataacat tgaattcaat tggcgatgcc gttattgtaa cagacaacaa tggtttttta
     1321 gttcgtatga atcctatcgc tgaggaattg actggttggg aaaacagtga aacaaccggt
     1381 aaacctcttg atgacatttt cagaatagta aactatctcg acaaaacgcc acttcctaat
     1441 cctgttcttc aggtaataca aaataaacaa ataattaatt tacagccgaa tgcacttctt
     1501 atatcaaaag acaatagtga ataccgtata agcgatagtg cagcacccat tattcgtgac
     1561 gatggatctc ttttgggagt tgtacttgtt tttcgagatg aaaccgaaaa aattaaaaaa
     1621 gacatgcaat taatacagtc acaaaaaatg gaggctgtag gtacccttgc tggaggattg
     1681 gcgcacgact ttaacaatgt actcggcgga ataatcggaa cagcttctct tttaaagaag
     1741 cagctttcaa ataatgacac tattgacaaa aacaaattac ttgatcacat ttcacttatt
     1801 gaacagagcg gcgaacaggc cgccgacatt gttcgacaac ttttaacaat ctcacataat
     1861 caagaattca ccttcgaaac cgttgacctt aatcaatcag taaaatcagt cagtgaaatg
     1921 gcaaaaaaca cttttgaccg atcaattagc atcaaaataa catatcttga taaacccgca
     1981 cttattaatg cagaccgtaa ccaaatcgaa caggttctac tcaatctttt tattaacgcc
     2041 tcacacgcaa tgacaataat gcgtcccgac gctgatattt ggggggggac tctctctgtg
     2101 gatattaggg agatatcgac gagcgatacg tcaggaaata gaataagaaa aattcgactg
     2161 gatgttcaag acagtggcac aggaatacgc aaagtagacc tgaaaaagat atttaccccc
     2221 ttcttttcga ccaagggaaa aggcaaagga agcggattag ggctttcaat ggtttacagt
     2281 attatagaaa aacatggtgg tgaaatttcg gtctattccg aaattaatag aggaacaaca
     2341 tttacgctgt tatttccttt gatcgaatca aaaacaactg aaaataaaaa agtacccaaa
     2401 aaaaatatta ttgaaagagg atccggttta atacttgtca tcgatgatga agaaatatta
     2461 cgtaagacat caaaagcaat gctagaagag tgtggttacg aagtactaac ggctaaaagc
     2521 ggtaacgagg gtatagcctt gtataatgaa aacaaagatc gtatctctgg aattattcta
     2581 gatatgatta tgcccgggct ttcaggtaaa gagacatatc agaaattaaa ggagattaat
     2641 cccgatgtga aagtaatatt agcttccgga tttaaacagg atgaaagaat agaatccgca
     2701 atgaagatgg gaatcaggga ttttatccaa aaaccctatt cgttgagtga gctttctctc
     2761 aaagcaaaaa gtttctaaac ccggataagc acaatttttg aatgacaata aaagcagaac
     2821 caacactata attttattta ttacatatat tatattatca ttttagctat ccaggatttc
     2881 aatccctgaa aaatcatctc tatagcaaca gttccaacaa atagtgctgt tatacgtccc
     2941 gcaatttcta tataccgctg aatcagggct tcacgtcgag tccgcacata atcgtgaact
     3001 cttttcaata tatagatggt tgcgatacaa agagccgttg ccgccaaaac agccagactt
     3061 cctataacaa ga
//
LOCUS       DAOTGZ010000288         3059 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_3064
            NODE_37874_length_3059_cov_4.366844, whole genome shotgun sequence.
ACCESSION   DAOTGZ010000288 DAOTGZ010000000
VERSION     DAOTGZ010000288.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868088
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 3059)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3059)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:22:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,785
            CDSs (total)                      :: 2,751
            Genes (coding)                    :: 2,732
            CDSs (with protein)               :: 2,732
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 12 of 19
            Pseudo Genes (internal stop)      :: 5 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3059
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_37874_length_3059_cov_4.366844"
                     /isolate="AMR_MDS_3064"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..597
                     /locus_tag="P5123_12985"
     CDS             <1..597
                     /locus_tag="P5123_12985"
                     /inference="COORDINATES: protein motif:HMM:NF014860.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRX17224.1"
                     /translation="GVEYDENGILFNRSLRTSASNIYACGDVTGGYMYTNKAEAQASV
                     AVVNALSSIRKKMDYSSLVWGLQTSPMVAHMGMTGSEAQARYGKFGIRIYKFPFKYSM
                     HAALRSESGGLAKIICRPSGRILGAHVFGDNADSIVHELQIAKTNGINFGKISLSMHI
                     FPSYGDVLTKPSLRSYIDQYSRNILINIVRALIPHRRR"
     gene            599..2143
                     /locus_tag="P5123_12990"
     CDS             599..2143
                     /locus_tag="P5123_12990"
                     /inference="COORDINATES: protein motif:HMM:NF018884.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HRX17225.1"
                     /translation="MEDAVSGVFFNEILSHINEYVYSVTLENNRILSVYHSPRSQVLL
                     GYSPEELSMDFALWKSLIYPEDRETVIGFLTYARQRSSTSAIEHRIVHRSGAVRWVTN
                     SCNIVVDAKTKTERLDGVVSDITERKKRVLQLTRLNRVVEQSPSAVVVTGVTGEIEYV
                     NPKCCQITGYNVDELVGQNPRILKSGDKSVEFYRELWNTISSGGEWKGEFKNRAKDGR
                     IFYEMASISPLRDEGGEIVNYIGVKEDITERKLAEIELTKREKELRKRNDKYVFELEQ
                     AQRLHRALMPKESPENEFISSVFLYRPMDEVGGDYFSFSEHMDGSTGLIVADVSGHGV
                     YAALYLTLIKHISEKIVNRLWNMPDRVLEDINNELVSTMELNFVTLLYGCFTYYPESS
                     CVSFHYANGGHTNSIVYYKNENQYELFNAKGTILGMFKEYQVESETLTLHSGDRLFFF
                     TDGLIELWDSSKKMLGYDGLTEILKRNSDNSITVLKNKIETEINSFCGGKPADDDILL
                     VIIEIK"
     gene            complement(2149..>3059)
                     /locus_tag="P5123_12995"
     CDS             complement(2149..>3059)
                     /locus_tag="P5123_12995"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="beta-galactosidase"
                     /protein_id="HRX17226.1"
                     /translation="YDYDALLNEAGDPTPKYYACQEVLSRYTKNEPLKKITPSDRIEK
                     KNIIFPKRALLFESIKHISTPILSPTPLTMEALEQDSGFILYRTKIDGPYEDAELKIL
                     GLHDRALIFINQKFYNTLYRNEISNKIVIDIPVKGAQIDILVENMGRIDYGPYIGEKK
                     GITHGVQLNNRFLFEWEIYPLPLDDLSALPFEINSTYKPETTPTFYSTNFSIERPRDT
                     FIDMDGWVKGVCFINGFNLGRYWQKGPQETLYVPGPLLRKGKNELTIFELHSPLELSI
                     ELVKQPRYGKSSQIKKSILSRLFSRT"
BASE COUNT          904 a          528 c          705 g          922 t
ORIGIN      
        1 ggtgttgaat acgatgaaaa tggaattctt tttaacaggt ctttgcgtac gtccgcttcc
       61 aatatatatg cttgtggaga tgtcacggga ggttatatgt acaccaacaa ggctgaagca
      121 caagcctctg ttgctgttgt aaacgcttta agttcgatac gtaaaaaaat ggattattcc
      181 tctttggtct ggggattgca gacaagcccc atggttgctc atatgggaat gacggggagt
      241 gaagcccagg cgcgttacgg taagtttggt atacgtattt acaagtttcc gtttaaatat
      301 tccatgcacg ccgcccttcg aagcgaaagt ggaggtttgg caaaaatcat ctgcaggcct
      361 tcgggacgga tactcggagc gcatgttttt ggtgacaacg cggattcaat tgtgcacgaa
      421 cttcagattg ccaaaacaaa cggtatcaat ttcggcaaaa ttagtctctc aatgcacatc
      481 tttccttctt atggagatgt gttgacgaag ccctctctgc gtagttatat tgatcagtac
      541 agccgtaaca ttctgatcaa tatagtgaga gctcttatac ctcaccgcag gagataagat
      601 ggaagatgcc gtatccggcg ttttttttaa tgaaatattg tcacatatta atgaatatgt
      661 ttacagtgta acgcttgaaa acaacagaat actgagtgtg tatcactccc cacgctcaca
      721 agtattgttg gggtactctc cagaagagct ctctatggat tttgctcttt ggaaaagtct
      781 tatatatccc gaagacaggg agaccgtaat tggattttta acatatgcca ggcaacgttc
      841 atctacttct gcaattgaac atcgcatcgt acatcgaagt ggagctgtga ggtgggtaac
      901 caacagctgt aatattgttg tagatgcaaa aacaaaaacc gaacgtctcg acggggtagt
      961 gtctgatata accgagcgaa aaaaacgggt tctgcagctt acaagactta atcgtgttgt
     1021 agagcagagt ccatcggctg ttgttgttac tggcgttacc ggagaaattg agtatgtaaa
     1081 tccaaaatgt tgtcaaataa caggctataa tgtggatgag cttgtggggc aaaatccacg
     1141 aattttgaag tcaggagaca agtcggtaga attttatcgt gagctatgga ataccattag
     1201 ttcaggtggc gagtggaagg gagaatttaa aaacagagca aaggacggta gaatctttta
     1261 tgaaatggct tcgatttcgc cattgcggga tgaaggtggt gagatagtaa actatattgg
     1321 cgttaaagaa gatattaccg agcgcaagct ggccgaaata gagttaacca aaagagaaaa
     1381 agagttacgt aaaagaaatg ataaatatgt ctttgaactt gaacaggcac agcggctgca
     1441 tcgcgcgttg atgccgaaag agagtccgga aaatgaattt atcagttcgg ttttcctgta
     1501 tcgtcctatg gatgaagttg gtggagatta cttctctttt tcggaacata tggacgggtc
     1561 aacggggctt attgtggcag atgtttctgg gcatggagta tatgcggcgc tgtatcttac
     1621 gctaattaaa catatttctg aaaagattgt taatcgtctc tggaacatgc cggacagggt
     1681 tcttgaggat ataaataatg agctagtatc taccatggag cttaattttg ttactcttct
     1741 ttacggatgc ttcacctatt acccagagag cagttgtgtc tctttccatt acgcaaacgg
     1801 tggtcatact aactcaattg tatattataa aaatgaaaat caatacgagc tttttaatgc
     1861 caaaggaaca attttaggca tgtttaagga gtaccaggtt gaaagtgaga ctctgactct
     1921 gcatagtggt gatagacttt ttttctttac cgacgggctt attgaactgt gggatagcag
     1981 taaaaaaatg cttgggtatg atggtttaac cgaaatattg aaacgaaaca gtgataatag
     2041 catcactgta ttgaagaata aaatagaaac tgaaattaac tctttttgcg gaggcaaacc
     2101 cgcggatgat gatattttac tggttataat cgagataaaa taaaatactc aggttcttga
     2161 aaacagacga gaaagaatag acttctttat ctgactgctt ttaccgtaac gtggttgctt
     2221 gacaagctca atagaaagct ctaacgggct gtgtagttca aaaattgtga gttcattttt
     2281 accttttcgc agaagaggcc ctggaacata taatgtctct tgagggcctt tttgccagta
     2341 gcgccctaga ttaaaaccat taataaagca cacacctttg acccaaccgt ccatatctat
     2401 gaaggtatca cgcggtctct ctatcgaaaa atttgtcgag taaaaggtcg gtgttgtttc
     2461 gggtttatat gtgctgttaa tttcaaacgg tagtgccgaa agatcgtcaa gaggtaacgg
     2521 gtaaatttcc cattcaaaaa gaaaacgatt attaagctga acaccgtgag ttatgccttt
     2581 tttttcaccg atgtatgggc cgtaatcaat tcgccccatg ttttcgacaa gaatatctat
     2641 ttgggcacct tttaccggaa tatcgataac aattttattt gatatttcgt ttcggtaaag
     2701 agtgttgtaa aatttttggt taataaaaat aagagctcta tcgtgaagac caagtatttt
     2761 tagttcagca tcttcgtaag ggccatcaat ctttgttcgg taaagtataa aaccggaatc
     2821 ttgttccaat gcttccatag ttaatggtgt tggagatagt ataggggttg aaatatgttt
     2881 gattgattca aaaagcaagg ctcgcttggg aaagataata ttcttttttt ctatgcggtc
     2941 tgatggtgtg atctttttta acggttcgtt ttttgtataa cgagatagta cctcctggca
     3001 ggcatagtat ttgggtgtag gatctccagc ttcgtttagc aaagcgtcat agtcgtaac
//
LOCUS       DAOTGZ010000289         3006 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_3064
            NODE_38766_length_3006_cov_3.869197, whole genome shotgun sequence.
ACCESSION   DAOTGZ010000289 DAOTGZ010000000
VERSION     DAOTGZ010000289.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868088
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 3006)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3006)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:22:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,785
            CDSs (total)                      :: 2,751
            Genes (coding)                    :: 2,732
            CDSs (with protein)               :: 2,732
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 12 of 19
            Pseudo Genes (internal stop)      :: 5 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3006
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_38766_length_3006_cov_3.869197"
                     /isolate="AMR_MDS_3064"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            271..>3006
                     /locus_tag="P5123_13000"
     CDS             271..>3006
                     /locus_tag="P5123_13000"
                     /inference="COORDINATES: protein motif:HMM:NF019335.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase regulatory-like
                     domain-containing protein"
                     /protein_id="HRX17227.1"
                     /translation="MNNLLSLLIKAKIKLKSNNQYIMLIRQIMLTSINNKKIKQKSVD
                     MFRSIICIPKNSIVKIMISIGAVFLLLLPISTVSQTNGRITGRVIDSSTGEAMIGVNV
                     VIREAGLYTITDMNGGYILNGVVPGRHEVQFIMPGYQPVRENINVNSGTNNLNISLSY
                     MTVGELVVTGKRVDNTEAALLSKRKNAAVAQDAISAEQISKSPDGDAADVAKRVIGVT
                     IVDGNTVFVRGLGERYSSVVFSGSTISSPNPEKRIVPLDIFPSSLLDNMIVIKTYSPE
                     MSGEFAGGIVQINPKDYPDKRIVKASVGTSIHSNTSINLFGFSTPGDDFYSYKGGKYD
                     FLGYDDGGRQLPSDVTDESLRNYDSDLKERIGEKLDTTYNPDKRDPYIPLSVAFTVGD
                     SYSLGSNTLGILVSGQFKESSKNKKMRYNRYLTDGEYDRWYDIHQSTYSTSTGALFSA
                     GLAGQSNKFRFTSCYSHQSEDTAAFYEGYNSDTLKSGDSSTRNEQKYLLQFTEQTLWF
                     NQLSSEHYIYNGIKAYLKGAFSFAERVEPDTRRATLVDSRNDGTYEVKGVEDLTHSFL
                     SHQDFVYEISPAISFPFKQWNGLDSKIKIGSGYTYRERNANSRFFSWLPTENANIDGT
                     GHDNPSGNIGSYFDDSSIVGQYSDDGYYYLEEKSSSNGSYEGDLTIAAGFAQFDMPLY
                     HNVRFLGGVRYEYADMNLLYYDSQESMMTDLESEPLEQHNIMPAANLTFGLTKKSNIR
                     LAASKTVARPDFREVTPAEYPSLVDNEKIVGNPDLKQTDIYNADLRLEYFPGTTEIIA
                     ISLFYKKMYNPIEVVMRSKGGDAVRYEYKNAEEADNTGIEFELRKGLGMFHDSISDFM
                     VTANASYIYSEIKVKDTDLVDYTNNERPLQGQSPYVLNFGINYDNKRSGSSLGLLY"
BASE COUNT          986 a          479 c          616 g          925 t
ORIGIN      
        1 tgttcttgat tgggcacgta ctgctaaatt ttatccggct attgcgccag atggaactgc
       61 ttttaaatgt cgtttctttt tgcctatgaa gtttaagtta gaataagata atataatctc
      121 ctttgaacgc tatacaagac ctcgtaagtt tacgaggtct tgtttttttt ctggatattg
      181 aaaattaaca taaaatatat cgtttctgta tttttaattc aaatcatttg tgtaaatgta
      241 aattaataat aatttatcag gaagaaaaaa atgaacaatc ttttgtcact gcttataaaa
      301 gcaaaaataa aacttaaaag taataatcaa tacataatgc tgataaggca gatcatgcta
      361 actagtatca acaataaaaa aattaaacag aaatctgtcg atatgtttcg aagcataatt
      421 tgtattccta aaaattcgat agtaaaaatc atgattagca ttggagcagt ttttctgtta
      481 cttttgccta tttctactgt gtctcagacc aatggaagaa taactggtcg tgtaatagac
      541 tcttctacgg gtgaagccat gataggtgta aatgttgtta ttagggaggc cggtttatat
      601 acaataaccg atatgaacgg aggttatatt ttaaatgggg ttgtgccggg aagacatgaa
      661 gtgcagttta ttatgccggg atatcaacca gttagagaga atataaatgt aaatagtggc
      721 acgaataatt tgaatatatc actatcatac atgacggtag gtgaattggt tgttacgggt
      781 aagcgggtag ataatacaga agcagcacta ctaagtaagc gtaagaatgc tgctgtagcg
      841 caagatgcga taagtgccga gcagatatca aagtctcccg atggtgatgc ggctgatgtt
      901 gctaaacgtg taattggagt caccattgtt gatggaaata cagtttttgt tcgaggtctg
      961 ggcgaacgat attcttcggt tgtattttca ggttcaacta tatcttctcc caatcctgaa
     1021 aagagaatag tccctttaga tatttttccg tcatctttgt tggataatat gatagttata
     1081 aaaacatatt caccagagat gagtggagaa ttcgcaggag gaattgttca gataaatcca
     1141 aaagattatc cagataaaag aatagtaaaa gcttcagtcg gaacatcgat tcattctaac
     1201 actagtatta atctatttgg tttctctact cccggagatg atttttattc gtataaaggc
     1261 ggtaaatacg attttttggg gtatgatgac ggaggccgcc aattgccgtc tgatgtaacg
     1321 gatgaaagtc ttcgaaacta tgattccgat ttgaaagagc gaattggtga aaaacttgat
     1381 actacctata atcctgataa gagggatcca tatattccat taagcgttgc atttactgta
     1441 ggagatagtt actcccttgg aagcaatact cttggtatct tggtttcggg tcagtttaaa
     1501 gagtcttcaa aaaataaaaa gatgagatat aacagatatt taactgacgg tgaatatgac
     1561 cgctggtatg atatacatca atcaacatat tctacatcta caggtgctct cttctcggct
     1621 ggattagctg ggcaatcaaa taaatttcga tttactagtt gttattcgca tcaatcagaa
     1681 gatacagcag cattttatga aggttataac agtgatactt taaaatctgg agattcttca
     1741 acgcgaaacg aacagaaata cctccttcag tttacggaac agacactgtg gttcaatcaa
     1801 ctaagcagtg agcactatat ctataacgga ataaaggcct atttgaaagg tgcattctct
     1861 tttgccgagc gtgtagagcc cgatactaga cgggcaacac ttgtagatag ccgaaatgat
     1921 ggcacgtatg aggttaaagg tgttgaagac ctgacgcatt cttttttaag tcatcaggat
     1981 ttcgtatatg aaatatctcc tgcaatttca tttccgttta aacaatggaa cggtcttgat
     2041 tcaaaaataa aaattggaag tggatacact tacagagaga gaaatgcaaa cagtcgattt
     2101 ttctcatggt taccaacaga aaatgccaat attgacggta caggtcatga taatccgtca
     2161 ggaaatattg gatcctattt tgatgattct tctatagtcg gacagtacag cgatgatggc
     2221 tactattacc ttgaagagaa atcaagttcg aacggaagtt atgagggtga tctaacaata
     2281 gctgccggct ttgctcaatt cgatatgcct ctgtatcata atgtgcgttt tttgggtgga
     2341 gtccgctacg aatatgccga tatgaacctt ctctattatg attcacaaga gagcatgatg
     2401 actgatcttg aaagtgaacc tttagagcag cataatatca tgcctgcggc gaatttgacc
     2461 tttggcctta cgaaaaaatc aaatataaga ttggctgcaa gcaaaacagt agcccgcccc
     2521 gatttcagag aagtgacacc tgccgaatat ccatctctgg ttgataatga aaagatagtg
     2581 ggtaatcctg atttaaagca gacagatatt tataacgcag accttagatt agaatatttt
     2641 ccaggcacaa cagaaattat tgcaatatca ttattttata aaaaaatgta taacccaatt
     2701 gaagtagtta tgcgctccaa gggtggtgac gcagttcgtt atgaatataa aaatgccgag
     2761 gaggcagata atacaggtat tgaatttgaa ctaagaaaag ggttaggtat gtttcacgat
     2821 tcaatttcag atttcatggt tactgctaat gcttcatata tatattcaga aattaaggta
     2881 aaagacacag atttggtaga ttatacaaat aacgaacgtc ccttgcaggg tcagtctcca
     2941 tatgtactga actttggaat aaactatgat aataagcgta gtggaagtag cttgggttta
     3001 ctttat
//
LOCUS       DAOTGZ010000290         2999 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_3064
            NODE_38891_length_2999_cov_4.176291, whole genome shotgun sequence.
ACCESSION   DAOTGZ010000290 DAOTGZ010000000
VERSION     DAOTGZ010000290.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868088
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 2999)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2999)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:22:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,785
            CDSs (total)                      :: 2,751
            Genes (coding)                    :: 2,732
            CDSs (with protein)               :: 2,732
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 12 of 19
            Pseudo Genes (internal stop)      :: 5 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2999
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_38891_length_2999_cov_4.176291"
                     /isolate="AMR_MDS_3064"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(130..468)
                     /locus_tag="P5123_13005"
     CDS             complement(130..468)
                     /locus_tag="P5123_13005"
                     /inference="COORDINATES: protein motif:HMM:NF017902.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlbF family regulator"
                     /protein_id="HRX17228.1"
                     /translation="MNEESINDNYEELVQIADELNTLIRSSEIYNEYSALKIVIEEDK
                     KLSEIYKKVMKAGETLEKEGDKAKEVIGEITEVPDIVKRFIDYQKKLADMLDKVINTV
                     SGSVMDKQDI"
     gene            662..2134
                     /locus_tag="P5123_13010"
     CDS             662..2134
                     /locus_tag="P5123_13010"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012744.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HRX17229.1"
                     /translation="MITILEQPKTKYSLDYYAGDPIFSGPVADLKKSAEKAKIKLKDR
                     TIWMINSTAQGGGVAEMLPGLITLMNDLGFNVRWGVINSDDPEFFIITKKIHNMVHGD
                     KSANVNFTENDAQLFEYINRKNADKLKSIIKKGDILVVHDPQPMHLGKIISEECGLLT
                     IWRCHIGLDKHNDATYAAWNFLNKYSSSYAHSIFTAPEYVPDFFRNNHSIIHPCIDPL
                     SHKNQDLTVSKLTGILCNSGLLPAYEPIATPDYKSRIKKILFDGSTGEPGETGLLFRP
                     IIAQISRWDRLKGWLPLLDGFIKLKQSKIVEDNSLHSKRHIKRLQLSRLVLAGPDPTA
                     VSDDPEGLEVVRQVKERFVSLDSELQKDISILLLPMESAKENSLIVNALQRCATVIVQ
                     NSIREGFGLTATEAMWKRKPVLVSSATGLKQQVINNKNGLMVTNPEDSDEISECLVKL
                     IKNPGMAYNLGRNAQRSVYDHFLVFRQISQYLELFTRLLN"
     gene            complement(2198..>2999)
                     /locus_tag="P5123_13015"
     CDS             complement(2198..>2999)
                     /locus_tag="P5123_13015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020619978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="family 43 glycosylhydrolase"
                     /protein_id="HRX17230.1"
                     /translation="YSITDFNSPITDHGTVLKLEDIPWASKQLWAPDAAYKNGKYYLY
                     FPAKDNDGLFRIGVATSDKPYGPFKPEPKPIEGSFSMDPALFIDDDESAYMYFGGLWG
                     GQLESWLSGNYYPGGLGPKADEPAIGPRFVRMSDDMLSFDGPIKEISLLDEDGKPILS
                     SDRERWFFEGAWVHKYKDTYYLSYSTGPTHLIVYATSDKPEGPFTYRGILLLPVSGWT
                     THHSTVEYNGQWYLFYHDSTMSEGKSHLRCIKYSPIKHNSDGTLELVK"
BASE COUNT          892 a          614 c          567 g          926 t
ORIGIN      
        1 aggcagaaag ctacaataag cactattccg ttcaggattt tggggctcca tcatcggccg
       61 aataatcttc cagcaaagag accggtcttt cggtctcttt gaattgcacc aaatattcag
      121 ttccggtttt cagatatctt gtttatccat caccgaacct gatactgtgt ttattacctt
      181 atcaagcata tctgcaagtt ttttctgata gtcaataaaa cgtttcacaa tatccggaac
      241 ttcggttatt tcgccaataa cctctttcgc cttatcaccc tctttttcca gagtctctcc
      301 ggctttcatg acttttttat aaatttcaga cagtttttta tcttcttcga ttactatctt
      361 taaagcagaa tactcgttat atatttcact agacctgatg agagtattaa gttcatccgc
      421 gatttgcaca agttcttcgt aattatcatt tatagactct tcattcatgt ttaactccac
      481 ttaaacaaca tgggctgctg tcgactgtat attatctctt catttgttac acctgcaatt
      541 tgctgtcaaa taattttaac attttgtaat tagctatata gcgttttcat tacacgattg
      601 cacaaaacaa aatgttgatg ccaaattaaa tccttagtat cattgttaat ggaggtcttt
      661 catgataact attctagagc aaccgaaaac taaatattct cttgattatt acgcgggtga
      721 ccctattttt tcaggccctg tagctgacct taaaaaatct gctgaaaaag cgaagattaa
      781 actaaaagat cgtacaatct ggatgataaa ttctactgct caggggggcg gtgtggctga
      841 aatgttacca ggactcatta cccttatgaa cgatctggga tttaacgtta ggtggggagt
      901 tataaactct gatgaccccg aattttttat tattacaaaa aaaatccaca acatggttca
      961 tggtgacaaa tcggctaatg taaatttcac agaaaatgac gcccagcttt ttgaatacat
     1021 taacagaaaa aatgcggata aactgaaatc aattatcaaa aaaggggata ttcttgttgt
     1081 tcatgatcca caaccgatgc atcttgggaa aataatctct gaagagtgcg gtctattgac
     1141 tatttggcgg tgtcacattg gattggataa acataatgat gctacctatg ccgcctggaa
     1201 ttttttaaat aaatactctt catcttacgc gcactctatt tttacggccc ctgaatatgt
     1261 tcccgatttt ttcagaaaca atcattcaat aattcatcca tgcatagacc ctctgtcaca
     1321 taaaaatcag gacctaacgg tttcaaaact taccggaatt ctttgtaaca gcggactttt
     1381 acctgcttac gagcctatag ctacgcctga ctataagagt agaataaaga agattctttt
     1441 tgatggttct acaggagaac caggagaaac cggtcttctt tttcgtccga taattgccca
     1501 aatatcccgt tgggacaggc ttaaagggtg gctgcctctt ctggacggtt ttattaagct
     1561 taaacagagc aaaattgttg aagataatag tttacatagc aaaaggcata taaagaggct
     1621 gcaactatcc cgtctggtac ttgctggtcc cgaccccaca gctgtatctg atgatcctga
     1681 gggacttgag gtagtacgtc aggttaagga acgttttgta agcctggata gtgagcttca
     1741 gaaagatata tcgattttac tgcttcccat ggaatcagca aaagagaatt ctctgattgt
     1801 gaacgcttta caaagatgtg caacagtcat agttcaaaac tctattcggg agggtttcgg
     1861 tcttactgca accgaagcga tgtggaaaag aaaaccagtt cttgtctcat cagcaaccgg
     1921 attgaaacag caggtgataa ataacaaaaa cggattaatg gtaacaaatc cagaggattc
     1981 tgatgaaata tctgaatgtt tggtcaaact cattaagaat ccgggtatgg cttacaatct
     2041 aggcagaaat gcccaaagaa gtgtctatga tcattttctt gtttttaggc aaataagcca
     2101 gtatttagaa cttttcacgc gattgcttaa ttaatggata atttggagtg ccttaacggc
     2161 accccaaaaa aaatttctaa ttgtgtttgc ttatgttcta cttaaccaac tcgagagtac
     2221 catctgaatt atgttttatt ggtgagtatt ttatacatcg cagatggctt ttaccttccg
     2281 acattgtgct atcgtgataa aacagatacc actggccatt atactctact gtcgagtggt
     2341 gagttgtcca ccctgatacc ggcaacagaa gaatacctcg gtatgtaaag ggtccctctg
     2401 gtttatcgga agttgcgtag actataagat gtgttggacc ggtagagtat gataaatagt
     2461 atgtatcctt gtatttatgc acccatgccc cttcgaaaaa ccagcgctca cggtcactag
     2521 acaaaatggg ttttccgtct tcgtctaaca gagatatctc tttaatagga ccatcaaaac
     2581 ttaacatatc atcactcata cgcacaaatc ttggtccaat agcaggctca tcagctttag
     2641 gcccaagacc acccggataa tagttccctg acagccaact ctccaactgc cctccccata
     2701 atcctccaaa atacatatag gcagattcgt catcatcgat aaatagagct ggatccatac
     2761 tgaaactacc ctctatcggt tttggctcgg gcttgaacgg accataaggt ttatctgagg
     2821 tagcaacacc gattcggaaa aggccgtcat tatcttttgc tgggaaatat aagtaatact
     2881 ttccgttttt gtaggcggca tcgggagccc agagctgttt tgaagcccat ggaatatctt
     2941 ctagtttcag aactgtgcca tgatcggtta tcggagagtt gaaatctgtt atagaatag
//
LOCUS       DAOTGZ010000291         2963 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_3064
            NODE_39505_length_2963_cov_5.165750, whole genome shotgun sequence.
ACCESSION   DAOTGZ010000291 DAOTGZ010000000
VERSION     DAOTGZ010000291.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868088
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 2963)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2963)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:22:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,785
            CDSs (total)                      :: 2,751
            Genes (coding)                    :: 2,732
            CDSs (with protein)               :: 2,732
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 12 of 19
            Pseudo Genes (internal stop)      :: 5 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2963
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_39505_length_2963_cov_5.165750"
                     /isolate="AMR_MDS_3064"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1607)
                     /locus_tag="P5123_13020"
     CDS             complement(<1..1607)
                     /locus_tag="P5123_13020"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRX17231.1"
                     /translation="MKLSSRYFVLVSFLLVIVSIACTNNKDNNNPDPILNEPLFNCGE
                     ETDNPDAIIIKESNCKVIIENDDPGYYKNTPGKVNGVTINAGDTICLKAGTRRGTNKG
                     FYFNGLTGTSANPIKIVNLNGFFDLQDDFGYSFKLDNCNHLRITGTGSQDYFYGIRIS
                     STMGNMMEVTGDSSNIEIDHVSFGSRNHIDDPDYFAGKECHDIRFKTDATPAGPCREA
                     ANAIMVKNDPACNSPYNGDTGFFMENIHIHHNRFEYVAQAIYVGYKNYEGTYRNCDDD
                     PTTGENGNETLTVPHDIKNIQVHNNCIMHTMKDGAQFKNLSSIKSRKINDPTNFTDPG
                     VESSIYNNHILFHSEKQLATQMSGLLVGTGSACNIYNNIIANGYGSAMELGIYAGKTY
                     NNLLINPGNPESNITAINSSEKCTDLESIQDSLTSTPGIRILNNDHVDFNDNSIERKI
                     EFYHNTIANGYNGIEIRDNGDFDIKIHNNLIIAARSFDEIFYKAYCEWGWTRENAFIR
                     SLATSFIKVAFGYMVNGETADTDAKNI"
     gene            complement(1917..2864)
                     /locus_tag="P5123_13025"
     CDS             complement(1917..2864)
                     /locus_tag="P5123_13025"
                     /inference="COORDINATES: protein motif:HMM:NF024634.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S dicluster domain-containing protein"
                     /protein_id="HRX17232.1"
                     /translation="MATKTPFKSFIVAPLMLNSVLIIASMIFVFAFKNTTPVPIFMIF
                     MLINTIFMTLYALVPLKYKNGFRLANMLIIGSFLFFLAQLLGRQNFQIEGFFFYLFAG
                     VIGGVIINYANGKLIGPLISGRSWCGWNCWTVMFLDLLPWKKNTTWQTSGIRYIKYIM
                     FALSLALTAVLVLIFKYTIVDTSPEAMATGTKTALIWGAVGNILYYVIGIALAVRFKD
                     NRAFCKYACPVSLFLKASNKFALLKIGTKKEKCTDCKTCSSVCPMSIDIPAYVKDGTR
                     VLSDECIMCMKCVAHCPENALFASVGLDISKKNQLIVIE"
BASE COUNT          862 a          512 c          577 g         1012 t
ORIGIN      
        1 ttgatgtttt tggcatctgt gtcggctgtt tcaccgttta ccatatatcc aaatgcaacc
       61 ttgataaaag atgtggcgag agatcgaata aaagcattct ctcgtgtcca accccattca
      121 caataggctt tgtaaaatat ttcgtcaaaa ctgcgcgcgg cgattataag gttgttgtgt
      181 atcttgatat caaaatcacc gttgtcacga atttcaattc cgttataacc gttagcaatt
      241 gtgttgtggt aaaactctat ttttcgctcg atagagttgt cgttaaagtc tacatggtca
      301 ttgttgagta ttctaatgcc cggagtcgat gtaagagaat cttgtatcga ctcaagatcg
      361 gtgcatttct ccgaagaatt aatagctgta atattgcttt caggatttcc ggggtttatc
      421 aggaggttgt tgtatgtctt tcctgcataa attcccaatt ccattgctga tccgtatcca
      481 tttgcaatta tgttattgta aatattgcaa gctgatcctg ttccaacaag cagtccgctc
      541 atctgagtag caagttgttt ttctgagtga aacaaaatat ggttattgta gatactactc
      601 tctacacctg gatcagtgaa gttagtggga tcgttaattt ttctagattt aattgaagat
      661 aggtttttaa actgtgcacc atctttcatt gtgtgcatta tacagttgtt atgtacctga
      721 atgtttttta tgtcatgagg tactgttaat gtttcgttgc cgttttctcc cgtagttgga
      781 tcgtcatcac agttgcggta ggtaccctcg taattcttgt aaccaacata tatagcctga
      841 gcaacatatt caaagcggtt gtgatgtata tggatgtttt ccataaaaaa acctgtatct
      901 ccattatacg ggctattgca ggcgggatca ttttttacca ttattgcgtt agctgcttca
      961 cggcatggtc cggcgggcgt tgcgtcggtt ttaaatctta tatcgtggca ctcttttcct
     1021 gcaaagtaat ccgggtcatc aatatggttg cgtgaaccaa aagatacatg atcaatttca
     1081 atgttcgatg agtctccagt tacttccatc atgttgccca tagtggacga aatacgtatt
     1141 ccatagaaat aatcttggct tccagtgccc gtgattctta aatggttgca gttgtccagt
     1201 ttaaatgagt aaccaaagtc atcttgcaga tcaaaaaaac cgttaagatt gacaatttta
     1261 attggatttg cactggttcc tgtcaagccg ttaaaataaa aacccttatt ggtgcctctt
     1321 cgcgtacctg ctttaagaca gattgtgtct ccggcattga ttgtgacacc gtttaccttt
     1381 cctggggtat ttttatagta cccagggtcg tcattttcaa ttattacttt gcagttggat
     1441 tctttgatta ttattgcatc gggattgtcg gtctcttcac cacagttaaa aaggggttcg
     1501 tttagtatcg gatcagggtt attgttatct ttattgtttg tacatgctat tgatacgatt
     1561 accagtaaaa atgataccag tacaaaatat ctggatgata atttcattac tactcctgtt
     1621 tgttgctcgg ggattgtatt ttaaagcaac gttgtagtaa ttatttgttt tggtaaagag
     1681 taaaaaaata tttaaactaa tatttgcaac aatatacaaa aaattaacca aacagggggt
     1741 ttagtttaat tgaattagcg atatatgaca ataatgatcg cttggagagg ttttgtaaac
     1801 cgggtattaa gaattgcttc caaaatagta acggtagaag ttcatatagt acagaattgt
     1861 tattattatg gaagcatttg ttttaaaatt ataaatattg catatttttg ttaatactat
     1921 tctataacaa tcaattgatt ctttttacta atatcgagtc caacagaggc aaatagtgcg
     1981 ttttcggggc agtgtgccac acacttcatg cacattatgc actcgtctga aagcactcgt
     2041 gttccatctt taacataagc aggtatgtcg atactcatag ggcaaacact gctacatgtt
     2101 ttacagtcgg tgcatttctc tttttttgtt ccaattttta aaagagcaaa cttgttagag
     2161 gcttttaaga aaagagagac aggacaggcg tatttgcaaa atgcacggtt atccttgaat
     2221 cttacggcca atgcaattcc tataacataa tagagtatgt ttcctacggc tccccagata
     2281 agggcggttt ttgttcctgt ggccatggct tcgggtgaag tgtcaacaat tgtgtatttg
     2341 aatatcagta ccagaacagc agttagtgct aacgataaag caaacattat gtatttaatg
     2401 tagcgaatgc cgcttgtttg ccaggtcgtg ttttttttcc atggcaaaag atcaagaaac
     2461 attactgtcc agcagttcca tccgcaccaa cttcttccag aaataagagg tcctattaat
     2521 ttaccatttg cgtaatttat gataactcca ccgattactc ctgcaaaaag gtaaaagaag
     2581 aaaccctcga tctgaaagtt ttgtcgacca agaagttgcg cgagaaaaaa tagaaaactt
     2641 ccaattatta acatgtttgc caaacggaag ccgtttttat attttaatgg aactaaggcg
     2701 taaagagtca tgaaaattgt atttatcaac atgaaaatca taaatattgg tactggagtt
     2761 gtgtttttaa aagcaaaaac aaatatcatt gatgcgataa tgagcacaga gtttaacatc
     2821 aaaggtgcta cgataaaaga tttaaaaggt gtttttgttg ccatattgtt gcctctgtcg
     2881 gaataattat ttaaatcatt ttgaactttt tgaagctatc tgaagcttga aaagccgatg
     2941 gaatatgatt cagttttaaa tat
//
LOCUS       DAOTGZ010000292         2941 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_3064
            NODE_39912_length_2941_cov_6.022523, whole genome shotgun sequence.
ACCESSION   DAOTGZ010000292 DAOTGZ010000000
VERSION     DAOTGZ010000292.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868088
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 2941)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2941)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:22:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,785
            CDSs (total)                      :: 2,751
            Genes (coding)                    :: 2,732
            CDSs (with protein)               :: 2,732
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 12 of 19
            Pseudo Genes (internal stop)      :: 5 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2941
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_39912_length_2941_cov_6.022523"
                     /isolate="AMR_MDS_3064"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(473..2221)
                     /locus_tag="P5123_13030"
     CDS             complement(473..2221)
                     /locus_tag="P5123_13030"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRX17233.1"
                     /translation="MNYKIKVTDEGNNQVEVLKKITENHYRIEWTFTLKKDSNPIVLS
                     EMYNDDCFVIVNWNAWVRAYSISKKELIYEKKFNGNISSSAALSKDNTELFTAYRCKD
                     GNFLDVISLTDFSTEKSILLPDDLNPGFLACGPGETVLFYKSDVDFGEKASHGYYTVN
                     LISEKISYTEMEYPQLDEFETAAPVISLKHNIGAMPLWDVEIKMDSNNEPLFIMKIML
                     FDLNSFKIIKSIPVREFRVEELSCRESASEETAEALLKKAGSSSNGLLSKFFQKTSSD
                     EDNIDLDEYYESMVEFISGNLYSIFFYDEKSFWVSFRGGIVRRVGFDGSLSPLFKTVS
                     LPDFSNEQMQTPDEILKTFHSHIGSVDNKRVILQAHNELYQMDINESELTSKEAVIEK
                     ALSEKKKEHVEISENDKKKIEDKDATIINVKNIKSNTGLLEALDQIVELTADIDEIKS
                     GHLLIFKVSDGEKELPEEEFFKKAVSIKGAANKIIKIIDNFTTYKEASRLYYNEETTA
                     LAYAVYYLTKSDPQYIDYAIKYLSVIDAEHDFFNGNMLLPMLFEKYSESHGSYLKEEL
                     SKLEDGQLWLEGYEDE"
     gene            2550..>2941
                     /locus_tag="P5123_13035"
     CDS             2550..>2941
                     /locus_tag="P5123_13035"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRX17234.1"
                     /translation="MTRARFYAYPNGRLVVAPWAVDESEKGIISLEIYIKHELSHVQL
                     YQNMSLIAAYYYPQWLLEGIAVYSTNQMGTSWYPDKIKTYEIIKNGNFFPPYLYKTNK
                     EDSIKLNTENRIAFMYSEFGCIVDFLIE"
BASE COUNT          914 a          560 c          460 g         1007 t
ORIGIN      
        1 tctggatctg taacgaaaac tcctgttaga tctgcatctt tatcttcgtt aaccagttcg
       61 tttgatagta tccaatacac atttgcctct ctattcacta aattgagttt caaaattttt
      121 ctacaaagca gcaagttctt cttttaaaaa agctcttcaa tacatttttc actaataaaa
      181 gtatttgtta aacagctcaa atatttatta agccatttac tttcagtgtc aggatccata
      241 ccatcagctt caagcacctc cttggcaaat taccgggcat catatattta tgatttaata
      301 atggttgcct ttttatcagg tggcagacaa aagccccacc tcaaacacag atcatcaagc
      361 agataaagca actattgctt atttgctgaa atatttcttt tattactttt ttcacctttt
      421 gctcacataa taaagggggt cagagccatt cgcgtgatcg aatgcaaaat cactattcat
      481 cttcataacc ttcaagccac aactgtccat cttcaagttt cgaaagttct tctttcaggt
      541 acgatccgtg tgactctgaa tatttttcaa aaagcattgg taaaagcatg tttccgttaa
      601 aaaaatcgtg ttctgcatca ataacagaga gatattttat tgcataatca atatattgag
      661 ggtcagattt tgtcaggtaa tagaccgcat acgctagggc tgtcgtctct tcattgtaat
      721 acaggcgaga cgcttcctta taagtcgtaa agttatcaat tattttaatt atcttatttg
      781 cggcaccttt tatagaaaca gccttcttaa aaaattcttc ttctggaagt tccttttcgc
      841 catcactcac tttaaaaata agcaggtggc cgcttttaat ttcatcaata tctgcagtta
      901 actccacaat ctgatctaga gcctccagta atcccgtatt agacttaatg tttttgacgt
      961 tgataatagt tgcatctttg tcttcaattt tctttttatc attttcagaa atttccacat
     1021 gttctttctt cttttcagaa agagcttttt caataacggc ttctttagat gtaagctctg
     1081 actcgttaat atccatttga tacagttcat tatgagcctg taatatcact cttttattat
     1141 caacagagcc aatgtgactg tggaaggtct tgagtatctc gtcaggagtc tgcatctgct
     1201 catttgaaaa atccggcagc gacacagttt taaaaagcgg cgagagtgaa ccatcaaagc
     1261 caactcgtct gactatccct cctcgaaatg aaacccaaaa tgatttttca tcataaaaaa
     1321 agatagaata gagattgccg gaaataaatt caaccatgct ttcatagtat tcatccaaat
     1381 ctatattatc ttcgtctgaa ctggttttct gaaaaaattt tgatagtaag ccgtttgaac
     1441 tgctgcctgc ctttttcagc aatgcttctg ctgtttcttc acttgcgctt tccctgcaag
     1501 agagttcttc aactcgaaac tccctgacag gaatactttt aataatttta aaagaattaa
     1561 ggtcaaaaag cattatcttc ataataaaaa gaggctcatt gttgctgtcc atttttattt
     1621 caacatccca aagaggcatt gctccgatat tgtgcttaag actaataacc ggagcagctg
     1681 tttcaaactc gtcaagctgg ggatattcca tttcagtata tgaaattttt tcactaatca
     1741 gattgacagt ataataaccg tgtgaagcct tttctccaaa atcaacatct gatttgtaaa
     1801 aaagaactgt ttctccaggg ccgcaagcta aaaatccggg attaagatca tcaggcagta
     1861 atatactttt ttctgtagaa aagtcggtta atgatattac atcaagaaag tttccgtcct
     1921 tacaccgata agctgtaaac agttctgtat tatcctttga cagggcagca cttgaactta
     1981 tattgccatt aaattttttt tcataaataa gctctttttt tgagatactg tatgctctta
     2041 cccaggcgtt ccaatttaca ataacaaagc agtcatcatt atacatctct gataaaacaa
     2101 ttgggttgct atccttttta agtgtgaaag tccattcgat cctgtagtgg ttctctgtaa
     2161 tttttttaag cacttctacc tgattgtttc cttcgtctgt tacttttatt ttgtaattca
     2221 tattacctct tattgtgaat atgtctttat cacttttttg ctgtaaaaag catatttgcg
     2281 gatttattaa taaaatcaat attatttaga tacttgcatt caagtactaa ttgcaatccg
     2341 gatttttaat agtcaactct ttcatcaaag gcattgcttt tgaattctgt gaacacagtt
     2401 ataacagtat aaaattactt ccatataaaa caggaagata attatacctt tcagaaagca
     2461 cttttataaa ttcatagaaa attggcattg aacagcacaa gccgaagata ttcattttct
     2521 ctgataaaaa gagctattta agacgaaatg tgactagggc gaggttctac gcgtatccta
     2581 atggccgcct ggtagttgct ccctgggcag tggatgaatc agaaaaagga atcatatcac
     2641 tggaaatcta tattaaacat gagctatcac atgtccagtt gtatcaaaac atgagtctta
     2701 tagcggcata ttattatccg caatggttgt tagaaggaat agccgtgtac agtacaaacc
     2761 agatggggac atcatggtat cctgataaga ttaaaacata tgaaattatt aaaaacggaa
     2821 acttcttccc gccttattta tacaaaacaa ataaagaaga tagtattaaa ctgaatacag
     2881 aaaacagaat tgcctttatg tattccgagt ttggatgcat tgttgatttt cttattgaaa
     2941 a
//
LOCUS       DAOTGZ010000293         2941 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_3064
            NODE_39920_length_2941_cov_3.748441, whole genome shotgun sequence.
ACCESSION   DAOTGZ010000293 DAOTGZ010000000
VERSION     DAOTGZ010000293.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868088
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 2941)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2941)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:22:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,785
            CDSs (total)                      :: 2,751
            Genes (coding)                    :: 2,732
            CDSs (with protein)               :: 2,732
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 12 of 19
            Pseudo Genes (internal stop)      :: 5 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2941
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_39920_length_2941_cov_3.748441"
                     /isolate="AMR_MDS_3064"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(194..433)
                     /locus_tag="P5123_13040"
     CDS             complement(194..433)
                     /locus_tag="P5123_13040"
                     /inference="COORDINATES: protein motif:HMM:NF012798.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HRX17235.1"
                     /translation="MPFLHIFRHSFASHLLMNGAGVKDVSVLLGHASVDSTVGYTHFT
                     VRSLKKIMKQYHPRENNLYIEIGKLPEIRSVINKS"
     gene            546..752
                     /locus_tag="P5123_13045"
     CDS             546..752
                     /locus_tag="P5123_13045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016876124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system HicB family
                     antitoxin"
                     /protein_id="HRX17236.1"
                     /translation="MRQVIIYPGEDGYFVAECPSLPGCITQGKTKEETIENIKEAIEG
                     YILTLQEDHLPIPAEHFDSMMVVV"
     gene            749..979
                     /locus_tag="P5123_13050"
     CDS             749..979
                     /locus_tag="P5123_13050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011362514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003729 - mRNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system HicA family
                     toxin"
                     /protein_id="HRX17237.1"
                     /translation="MSGLPRLSGKECVKILERGGFYFKRQHGSHIILRRTDPYAQVVV
                     PNHKELDRGTLRSIIRQAGISINEFYELIQKH"
     gene            complement(1270..1443)
                     /locus_tag="P5123_13055"
     CDS             complement(1270..1443)
                     /locus_tag="P5123_13055"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRX17238.1"
                     /translation="MIFVSMRRKKEAELVKLILEKSPDMKIDYKFYKLVKKYYGLDYS
                     NNIIEGSYPFFFE"
     gene            complement(1691..>2941)
                     /locus_tag="P5123_13060"
     CDS             complement(1691..>2941)
                     /locus_tag="P5123_13060"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRX17239.1"
                     /translation="GWNRYMYCAGNPVLYKDPSGHSWQTMQQEFRKGILDSQDTDSRL
                     QTEQKLFDLERTGVDCVDSLKSKLHDNRVESGWSTDMKGADQSALDSYIEDVYKGSTP
                     WFAKDKSNGPVKPVDHPNAYVSSGYYRNDKKWKPALHGAIDVSTGKNEGVQAKAMWKV
                     KLVYKDYQTNKSGTSGYGNRAIMEILEGQYKGSFLLYGHLAKPVAHEKGAVIQKGEEV
                     GIVGGTGRKKGYPIRRSEMFPLNKIKVISIVIIMFVFVITPAEDSNNLYKKLEQRIVE
                     ELGNGYEIIRKEKIHFKENKDVFIDDYKDKRASFNLKAFDVYQYNGKFLIACACINTV
                     NRDKKAAVIIFDSDLQFKKLIMKQVIWKKVYDLFPSLYKVNNAIILDIWYKNGTDWVD
                     SYEFDTKNNNFKQVPSAMDTDWPI"
BASE COUNT          960 a          617 c          420 g          944 t
ORIGIN      
        1 tatcgttact ctcaccttta tctaaccagg tctccccgta tgggaggtac tcgaacctct
       61 ctaaatctga agaaaaaatc accaacaaag gcgcaattaa tgagatttca agtcagatcg
      121 gggtttatgc cctgcaaaat cggtaaaact ttcaaagatc gatatcgaga ccatacaaaa
      181 caaaagcaaa gtgtcaagat ttattaatta cagatctaat ttcgggtaac ttaccaattt
      241 caatatacag attgttctct cggggatgat actgtttcat tatctttttt aagctgcgta
      301 cagtaaaatg tgtgtagcct actgtactgt caacagaagc atgaccgagc aaaacagaaa
      361 catcttttac ccctgcaccg ttcattagta aatggctggc aaagctgtgc cgaaatatat
      421 gtaaaaacgg catcactttt tagacttgac taaaatcaat ttcttaatta gactttaata
      481 atgatattaa tcataaagat ctgctaaacc aacaaactac tgtatctgca taaaaggata
      541 aaattatgag acaagttata atataccccg gagaagatgg atactttgtt gccgaatgtc
      601 ccagcttacc cggctgtatc acccaaggaa aaacaaaaga agaaaccatc gaaaatatca
      661 aagaggcaat tgagggttac attctaacac tgcaggaaga ccatcttcca ataccagccg
      721 aacactttga ctccatgatg gttgttgtat gagtggatta cctcgattat caggtaaaga
      781 atgtgttaag attttagaaa gaggcggatt ttactttaag cggcaacacg gaagtcatat
      841 cattctgcga agaactgatc catacgccca agttgtcgtt cccaaccata aggaacttga
      901 ccggggcact ctccgatcaa tcataagaca agccggcatc tctatcaatg aattctacga
      961 gctgattcag aaacactaaa aaaatttcat tttttttata catcatatat ggtagttttc
     1021 ttacatataa gtgctattct aaagccaaaa aaatcacgat aaattctgta attttatatt
     1081 tgctacccta aaccaaccaa cctcatcaca ctttcgcata attgataagg caagcgacgt
     1141 cgccgactta ccaaaactac aacaccacat aaaaatcggc gatgcgcgaa gcgaacgttt
     1201 gctgacaatt atgcgacagg tgatagtgaa agagtacgac tcaagtatgt agagaccaaa
     1261 agttgtgatt tactcaaaga aaaaaggata actgccttct attatattgt tagaataatc
     1321 aagaccgtaa tatttcttta ctaatttata aaatttataa tctattttca tatcaggact
     1381 tttttcaagt atcagtttta ccagttcggc ctcttttttt cttcgcattg aaacaaaaat
     1441 cattttcgta ttacctttgt aattgtgcat taaaaccata gtgctaatac ctgcaagaaa
     1501 aggtgtccat ctgtaaacta gctaaattaa catccaatga agattgttaa atgataaaca
     1561 taattccgta atggatggcc gtattgaacg gaatacgcat ttacaccaaa aagcggaaag
     1621 acccgattat tacaatttat aaaatatttt tcactctctt aaaaattaat cttatcagct
     1681 atcaattaat ttaaattggc caatctgtat ccatcgcaga ggggacttgt ttaaaattat
     1741 tattcttagt atcaaattcg tatgaatcaa cccaatcagt tccattttta taccagatat
     1801 caagtattat agcattattc actttataca atgacggaaa taaatcataa acctttttcc
     1861 atataacctg tttcattatt aactttttaa attgcaaatc gctatcaaat attattactg
     1921 cggccttttt atctctattt acagtattta tacaagcaca tgctattaaa aattttccat
     1981 tatattgata tacatcaaaa gccttcaaat taaagctagc tcttttatcc ttataatcat
     2041 caataaatac atctttattt tccttaaaat gtattttttc ttttcttatt atctcataac
     2101 cattgcctaa ttcctcaact attctctgtt caagtttttt atataaatta ttactatctt
     2161 cagcaggtgt aataacaaat acaaacataa taattactat agaaattacc ttaattttat
     2221 ttagcgggaa catctctgac ctccgtattg ggtatccttt ttttcttcca gttccaccaa
     2281 caatacctac ttcttctcct ttttgtatta ccgctccctt ttcatgtgca acgggtttag
     2341 ccaaatggcc atatagtaag aaactcccct tatattgccc ttctaaaatt tccataatcg
     2401 cacgattacc ataaccagat gttccactct tatttgtttg atagtcctta tatactaatt
     2461 taactttcca catcgctttt gcttgaacac cttcattttt tccggttgat acatcaatag
     2521 ctccatgtaa agcaggtttc cattttttgt cattcctgta atacccggaa ctaacatatg
     2581 catttggatg atccacaggt ttaactggac cattactttt gtccttagca aaccacggag
     2641 tactaccctt ataaacatcc tcaatataac tatcaagagc tgattggtca gcccccttca
     2701 tatcggtcga ccaaccgctt tcaactcggt tatcatgaag ttttgatttc aaagaatcta
     2761 cacaatcaac accggttctt tctaagtcga agagcttctg ttcggtctgc aaacgactgt
     2821 cagtgtcctg actatcaagt ataccctttc gaaactcctg ctgcatggtt tgccagctgt
     2881 gcccactcgg atccttatac aacaccggat tcccagcaca gtacatatac cgattccacc
     2941 c
//
LOCUS       DAOTGZ010000294         2911 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_3064
            NODE_40464_length_2911_cov_3.656863, whole genome shotgun sequence.
ACCESSION   DAOTGZ010000294 DAOTGZ010000000
VERSION     DAOTGZ010000294.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868088
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 2911)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2911)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:22:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,785
            CDSs (total)                      :: 2,751
            Genes (coding)                    :: 2,732
            CDSs (with protein)               :: 2,732
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 12 of 19
            Pseudo Genes (internal stop)      :: 5 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2911
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_40464_length_2911_cov_3.656863"
                     /isolate="AMR_MDS_3064"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(54..2198)
                     /locus_tag="P5123_13065"
     CDS             complement(54..2198)
                     /locus_tag="P5123_13065"
                     /inference="COORDINATES: protein motif:HMM:NF012464.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000150 - DNA strand exchange activity
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HRX17240.1"
                     /translation="MVKDYKFLDQLRQAKEQRFQKGKKKRVALGVRVSTSKQKKDDAN
                     KPQKDNIPIPVQREALLEWINSNPNWEPAMVNGKILEYVEVKSAYKMTRSQREVLKKI
                     ISDADKDLYDVIVFFKHDRVSRIGNEYTGILQDLWDRGIEPWDYEKKQCLKMQTAPDR
                     IVRSIEGELAQSESENTSFRVTEHMKSYAAEGLWMGGVQPYGYKYKEITSEHLMDLQT
                     GKKKKLRIGIEKNPEEEEIVRRIIELAATGHGSSKICRIINNPPYNYRKRNGKPFDHA
                     TVLNIVKNPICIGKPAWGKTSTQKRYFKRLPPEEWVLPEVPVESLRIVEDSVYEEAIR
                     QIEERKTQVLKGKIISHRSLAAERLLSGIAFCGYCGEPLLSHTYANGEYKLEGYICRS
                     KKRKSLCSSKHSFIRASVLDRLIIDSVSSLFSEKFFEKDLVKKKVKETYLKATQQNSL
                     EVARLKSSINKLEKLKKHYIGQYNLAITGEKTTIPESIISEQLDDIQDQLQEALSRLN
                     SIIIVQPHLAPFDERQLEPIIDELLNWDTAFRTVPVETQKYMLKQITDSITVYDTEVV
                     IQYNLNINHMLNSISPEVASVVPGVDMRIVKHISYIYTNKARSLGIEYWQKVQEWEKR
                     KGLPFGEIIANMRRTKTPQQIIEELGVSRGFLYYNFGEYMDRIKYIENKEGKKIREVI
                     IEKKENGMTMKEISEDLGLSISCLYHNLKHKN"
     gene            complement(2201..2461)
                     /locus_tag="P5123_13070"
     CDS             complement(2201..2461)
                     /locus_tag="P5123_13070"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRX17241.1"
                     /translation="MSRGNPAFFILTVKLPETGEIYPNEKKEENKMSMSNSIKTVQTI
                     TSEQTLLSKTTENASKLTDTFLDAVVNVIVHRYIVATEEKKD"
     gene            complement(2474..2731)
                     /locus_tag="P5123_13075"
     CDS             complement(2474..2731)
                     /locus_tag="P5123_13075"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRX17242.1"
                     /translation="MIVIVAENNSSINECSRVFIDQINRIIGVSNGQNVVLSEYPNLR
                     QAQYSMVMIATAIANKLPIYVMESQKYYPDSYITEDLYPSV"
BASE COUNT          758 a          609 c          458 g         1086 t
ORIGIN      
        1 ctattatatc ataaatacac atttatatat tatgtacata caacaagaaa agcttaattt
       61 ttatgtttta aattatgata taaacaactt atacttaatc caagatcctc agaaatctct
      121 ttcatggtca taccgttttc ttttttttca ataataactt ctctgatttt cttcccttct
      181 ttattttcaa tatacttaat cctgtccatg tattcgccaa aattgtaata cagaaatcct
      241 cgtgagaccc ctaattcttc tataatttgt tgcggagttt ttgttcgccg catatttgcg
      301 attatctctc caaaaggcaa tccctttctt ttttcccact cctggacttt ctgccagtat
      361 tcgattccta atgaccttgc tttattggta taaatatacg aaatatgctt cactatacgc
      421 atatctacac ctggaacgac tgaagccact tctggtgata ttgaattaag catatgatta
      481 atattcaggt tatactgtat aaccacttca gtatcatata ccgtaattga atcggtgatt
      541 tgttttaaca tatatttttg agtttcaaca ggaacagttc taaaagcagt gtcccagtta
      601 agcagctcat ctataattgg ctctaattgc cgttcatcaa acggtgccag atgcggttgg
      661 actataatta tactatttaa tctgctcaag gcttcttgta attgatcctg gatatcatct
      721 aactgctcac taatgattga ttcgggtata gtggtttttt ctcccgtgat agctagattg
      781 tattgcccaa tatagtgttt tttgagtttt tccagtttgt ttatcgatga tttcagccgg
      841 gctacttcca gggaattttg ttgagtagct tttaaatatg tttctttaac cttttttttc
      901 actaaatctt tttcaaagaa tttttcacta aacaaactgg aaacagaatc tataatcaat
      961 ctatctaata cgcttgcacg aataaaactg tgcttagagc tacataaaga ttttcgtttt
     1021 ttactcctgc agatatatcc ctcgagtttg tattctccgt ttgcatatgt gtgtgacaac
     1081 aaaggttccc cgcagtatcc gcaaaaagca ataccgctta acaatctttc agcagccagt
     1141 gatctgtgtg aaattatctt gccctttaat acttgtgttt ttctttcttc aatctgcctt
     1201 atcgcttctt cgtatacact atcctcaact attcgaagtg attcaacagg cacttcgggt
     1261 aacacccatt cttcaggagg cagtctttta aaatatcttt tttgagttga ggtttttccc
     1321 caagctggct taccaataca aatcgggttt ttaacaatat ttaaaacggt agcatggtca
     1381 aatggctttc cgtttctttt tctatagtta tatggaggat tatttattat tctacagatt
     1441 ttactggagc catggcctgt tgctgccagt tctataatcc ttctaactat ttcttcttct
     1501 tccgggttct tctcaatacc gattcgcagt ttctttttct tgcccgtctg tagatccatt
     1561 agatgttccg atgtaatttc tttatattta tagccgtatg gctggactcc gcccatccat
     1621 aaaccttcag ctgcgtatga cttcatatgt tcggtgacac gaaaagaagt attttcactt
     1681 tccgattgtg ccaattcacc ttcaatgcta cggactattc tatcaggcgc agtctgcatt
     1741 tttaaacatt gttttttctc ataatcccac ggttctatac cgcgatccca taaatcttgc
     1801 aggataccag tatattcatt ccctattcgg gaaacacggt catgcttgaa aaatacgata
     1861 acatcataca ggtctttatc tgcatcactt ataatctttt taagcacttc gcgttggctg
     1921 cgggtcattt tataagctga tttaacttcg acatattcta atattttgcc attaaccatc
     1981 gcaggttccc aattaggatt gctatttatc cattctaata gtgcttctct ttgaaccggg
     2041 ataggaatat tatccttttg tggcttattt gcatcatctt ttttttgttt agaggtacta
     2101 actcgtactc cgagggcaac ccgttttttt ttgccttttt gaaatcgttg ttcttttgcc
     2161 tgccttaact ggtcaaggaa tttataatct tttaccatat tcagtccttt ttctcctcag
     2221 tagccactat ataccgatgt actatcacat ttacaacagc atccaaaaat gtatctgtca
     2281 gtttcgaggc attttcagtt gttttactca acagtgtttg ttcagaggtt attgtttgga
     2341 cagtttttat tgagttagac atcgacattt tgttttcctc ctttttctcg tttggatata
     2401 tttcccctgt ttcgggaagt ttaaccgtaa gaataaaaaa agccgggttc ccccggctca
     2461 ttcaagagtt tctctacaca gaaggataga gatcctcagt tatatatgaa tctggataat
     2521 acttttgtga ctccattaca tatataggca gcttattggc tatggccgtc gctatcatta
     2581 ccatgctata ttgcgcttgt cttaaattgg gatactcact taaaacaacg ttttgtccgt
     2641 tggatacccc tattatccgg tttatctgat caataaaaac tcgactgcac tcattaatgc
     2701 tactgttgtt ttccgccaca ataacaatca tggaacataa cctccttccc cgacttaacg
     2761 ggattttaag tccggcccgt aaagggcggg tttattacaa atcaaactca caatcaagag
     2821 ctaaactggc atcttcgaag ccgttttcat tgagccagtt gctaatttgg ttggctgttt
     2881 cttcatcgaa atttttcgta ccatttaact g
//
LOCUS       DAOTGZ010000295         2910 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_3064
            NODE_40475_length_2910_cov_4.009107, whole genome shotgun sequence.
ACCESSION   DAOTGZ010000295 DAOTGZ010000000
VERSION     DAOTGZ010000295.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868088
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 2910)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2910)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:22:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,785
            CDSs (total)                      :: 2,751
            Genes (coding)                    :: 2,732
            CDSs (with protein)               :: 2,732
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 12 of 19
            Pseudo Genes (internal stop)      :: 5 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2910
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_40475_length_2910_cov_4.009107"
                     /isolate="AMR_MDS_3064"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..2101)
                     /gene="carB"
                     /locus_tag="P5123_13080"
     CDS             complement(<1..2101)
                     /gene="carB"
                     /locus_tag="P5123_13080"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015942713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006807 - nitrogen compound metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbamoyl-phosphate synthase large subunit"
                     /protein_id="HRX17243.1"
                     /translation="MPKRDDIKKVLIIGSGPIVIGQACEFDYSGTQACKALRSLGYEI
                     VLVNSNPATIMTDPEMADYTYIEPLNDYALTEIIKKEKPDALLPNLGGQTALNLSLTL
                     ANSGVLDKYNVKVIGVNLKAIEKGEDRTEFKNSMERLGIDMPRSKAVNTVEEAETIAD
                     ELGYPVVIRPAYTMGGTGGGLVYNLEELRIIAARGIAASMVSQILVEESVLGWEELEL
                     EVVRDSKNQMITVCFIENVDAVGVHTGDSFCTAPMLTISEELQKKLQEYSYRIVEDIG
                     VIGGTNVQFAHDPKTGRVVVIEINPRTSRSSALASKATGFPIAFVSALLAGGLTLDEL
                     PYWREGTLEKYTPWGDYVVVKFAKWAFEKFDGLKDKLGTQMQAVGEVMSIGKNYKEAL
                     QKSIRSLETGRYGLGFAKDYNSKTAEELLVMLNQPSSERQFMMYEALRKGVSVQTLSE
                     LTYIKAWFIEQMKELVDLEEKIIKNRDNLPDELFKAAKMDGFSDKYLAKITGKTEDDI
                     RAKRLTLGIEESWEPVPVSGVNDAAYYYSTYNALNQVVPDNKKKIMVLGGGPNRIGQG
                     IEFDYCCVHAAFAIRDEGLDSIMVNCNPETVSTDYDTSNRLYFEPLTVEDVLSIYNKE
                     KPYGVICQFGGQTPLNIADELKRNGVNIIGTSPETIDLAEDRDQFRKIMEKLEIPQPP
                     SGMASTEDEAIAIAAKIG"
     gene            complement(2410..>2910)
                     /gene="ung"
                     /locus_tag="P5123_13085"
     CDS             complement(2410..>2910)
                     /gene="ung"
                     /locus_tag="P5123_13085"
                     /EC_number="3.2.2.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008759768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004844 - uracil DNA N-glycosylase
                     activity [Evidence IEA];
                     GO_function: GO:0016799 - hydrolase activity, hydrolyzing
                     N-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0006284 - base-excision repair [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uracil-DNA glycosylase"
                     /protein_id="HRX17244.1"
                     /translation="DKVNVVIVGQDPYHGEGQANGLCFAVNDGVRKPPSLLNIFKEIE
                     DDLGRKPSADSTLVHWVNQGVFLINATLTVRAHQAASHTNMGWEVFTDAVLRHLTQQK
                     SNLVFILWGSYAQKKGAFIDSRRHLVIKSAHPSPLSAFRGFFGSHPFSKANDYLVSLG
                     KSAIDW"
BASE COUNT          834 a          719 c          528 g          829 t
ORIGIN      
        1 accctatttt cgctgctatc gctattgcct catcctcagt gctcgccatg cccgatggag
       61 gctggggaat ttcaagtttt tccattatct tgcgaaactg atcacgatcc tccgccagat
      121 caattgtctc gggagaagta ccaataatgt taacgccatt tcgcttaagc tcatctgcaa
      181 tattcagagg agtctgccct ccaaactgac atattacacc gtaaggtttt tccttgttat
      241 aaatgctcaa aacatcttcg acagttagag gctcaaaata gaggcggtta gacgtatcgt
      301 agtcggtaga tactgtttca gggttacagt taaccatgat ggaatcgagc ccctcatccc
      361 gtattgcaaa agccgcgtga acacagcaat agtcaaactc tatcccctga ccaatacgat
      421 tgggaccgcc ccccaaaacc ataattttct ttttgttgtc gggcacaacc tgatttagag
      481 cgttatatgt cgaatagtaa taggcggcat cattcactcc gctaacagga acaggctccc
      541 aagactcttc gataccaaga gtcaaacgct ttgcacggat atcgtcttca gttttacctg
      601 tgatttttgc caaatacttg tcagaaaagc cgtccatctt agcagcttta aaaagctcgt
      661 ccggcaaatt atccctgttt tttattatct tctcttcaag atccacaagc tctttcatct
      721 gctctatgaa ccaggctttt atatatgtaa gttcgctcag tgtttgcacc gaaactccct
      781 tgcgaagcgc ctcgtacatc ataaactggc gttcacttga aggctgatta agcattacaa
      841 gaagctcttc tgctgtttta ctgttatagt ctttagcgaa accaagcccg taccgaccgg
      901 tctcaagaga tctaatcgat ttttgcaatg cctctttata atttttacca atactcatga
      961 cttcgccaac agcctgcatc tgagtgccaa gcttatcttt tagaccgtca aatttttcaa
     1021 aagcccattt cgcaaatttg acaacaacat agtcacccca tggcgtatat ttctccaaag
     1081 ttccctctcg ccaataaggc agctcgtcaa gggtcagccc accggcaagc aaagccgata
     1141 caaacgctat tggaaaaccc gtagctttcg atgcaagagc cgacgagcgt gacgttctag
     1201 gattaatctc gatcactacc acgcgtcctg ttttagggtc atgggcaaac tgaacatttg
     1261 ttcctccaat taccccgata tcctcgacaa tacggtacga gtactcctgc aatttttttt
     1321 gcaactcttc cgaaatcgta agcattggtg cagtacagaa cgagtcaccg gtgtgcacac
     1381 caacggcatc aacattttcg ataaaacaga ccgttatcat ctggttttta gaatcacgaa
     1441 cgacctcaag ttccaactct tcccaaccga gaaccgactc ttccacaaga atctgactta
     1501 ccatactcgc agcaattccc ctcgcagcga taattcgtag ttcttcaaga ttataaacaa
     1561 gaccaccgcc tgttcccccc atggtatatg ccgggcgaat tacaaccggg taccccaact
     1621 catcggctat ggtttctgcc tcttccacgg tattaacagc cttacttctt ggcatatcaa
     1681 taccaaggcg ctccatggaa tttttaaact cggtgcggtc ttcacctttt tcgatggcct
     1741 taagattaac accgatcact tttacattat atttatcaag cacaccgctg ttagccagag
     1801 tcaaagagag attgagagct gtctgaccac caagatttgg caaaagagcg tcgggcttct
     1861 ctttttttat gatttcggta agagcataat cgttaagagg ctctatatag gtgtaatcgg
     1921 ccatctcagg atcggtcatt atagtggcgg gatttgagtt taccaaaact atctcgtatc
     1981 ccaaagaccg caaagcctta caggcctgtg ttccagaata atcaaactcg caggcttggc
     2041 cgataactat cgggccagag ccaataatca gtactttttt aatatcatct ctttttggca
     2101 tatagcatat ccatcacatt tattatattt tatacaaaat tctgcaaact agaaaaccag
     2161 catctaataa cacttgattt aattctgttg ctgtatttca tagctttctc ctgtttattt
     2221 caggcgtcaa ctatgttggc acacagggct tccatttttg tctgacttcc gacaaaaaag
     2281 atcaacaact caaattactt tatttgaaat taaaaaaaag taaatttttc agtcaatcct
     2341 ttttatatag attatacaag tcgcaagtcc tgtgcaaaaa tacaaacaag ctaatagaaa
     2401 caacacttct caccaatcaa ttgcagactt tcccaaagat acaagatagt cattagcctt
     2461 tgaaaaagga tgagaaccaa aaaaaccgcg aaaagccgaa agaggcgatg gatgtgcgga
     2521 cttaataaca agatgccttc tcgaatcgat aaaggcccct ttcttttgtg catatgatcc
     2581 ccatagtata aaaacaagat tggatttttg ctgtgtaaga tggcgtaaaa ctgcatcagt
     2641 gaaaacttcc caccccatat ttgtgtggct ggccgcctga tgagctcgta ctgtaagtgt
     2701 agcattaatc aggaaaaccc cctgattaac ccagtgaaca agtgtagaat cggcacttgg
     2761 tttacgcccc agatcgtctt cgatctcttt aaatatattc aaaagagacg gcggtttacg
     2821 tacaccatcg ttaacagcaa aacagagacc gttagcctgt ccctctccat gatagggatc
     2881 ttggccaact ataaccacat tgaccttgtc
//
LOCUS       DAOTGZ010000296         2900 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_3064
            NODE_40654_length_2900_cov_5.150439, whole genome shotgun sequence.
ACCESSION   DAOTGZ010000296 DAOTGZ010000000
VERSION     DAOTGZ010000296.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868088
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 2900)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2900)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:22:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,785
            CDSs (total)                      :: 2,751
            Genes (coding)                    :: 2,732
            CDSs (with protein)               :: 2,732
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 12 of 19
            Pseudo Genes (internal stop)      :: 5 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2900
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_40654_length_2900_cov_5.150439"
                     /isolate="AMR_MDS_3064"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(34..468)
                     /locus_tag="P5123_13090"
     CDS             complement(34..468)
                     /locus_tag="P5123_13090"
                     /inference="COORDINATES: protein motif:HMM:NF012304.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRX17245.1"
                     /translation="MYTDGSCNPAKKLGAWAAIILMFGEEIVLSGTKMTSSHNAMELL
                     AAIKALEYVEKMSPLQELVLYSDSQYVVGAVTRMDALIASDFITGKGTERKNRELLET
                     FFLLIQKLSPRLSKVAAHQKKTAKRNINREVDKLARKLVRKS"
     gene            629..1318
                     /locus_tag="P5123_13095"
     CDS             629..1318
                     /locus_tag="P5123_13095"
                     /inference="COORDINATES: protein motif:HMM:NF013357.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003796 - lysozyme activity [Evidence
                     IEA];
                     GO_process: GO:0009253 - peptidoglycan catabolic process
                     [Evidence IEA];
                     GO_process: GO:0016998 - cell wall macromolecule catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GH25 family lysozyme"
                     /protein_id="HRX17246.1"
                     /translation="MWLLLLSLLLASCSSYRNIRHNYISFSKHPVQGLDVSRHQGEID
                     WAKVSISKFTFVFIKATEGGDLVDKKFAYNFNQAQQHGLIVGAYHFFSFKRTGKEQAK
                     NFISTVPNIPGMLPPVIDLEYMGRSNEKDSKENLHRELDIYIDEIEKAYNVKPIIYTN
                     YRFYRRYLEGHYIEYAIWICDYYKLPQLPDGRPWLFWQYSDRGKVAGIRGFVDFNVFN
                     GEKEELMNFVK"
     gene            complement(1372..>2900)
                     /locus_tag="P5123_13100"
     CDS             complement(1372..>2900)
                     /locus_tag="P5123_13100"
                     /inference="COORDINATES: protein motif:HMM:NF015983.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HRX17247.1"
                     /translation="IKDMNNIETDFLDILANPNFKHVRDNKTKPPIYFGMKVSEIIDY
                     VKSYSPACIAITCTANYFAKDTIKIINLIHRNFPDIIILIGGPDATNDNQMYLEKAPG
                     ITAVIMREGEETFREIIESLINSKSIFGIKGIAYKKNGKIKINPQRPYIDDLDRYKCD
                     YEVLDLEKYFNINKEGFPSRLIYKSPGSHRSIDIVTSRGCQMNCSFCCIHLHMGKKVR
                     AHSVQYVLDEMELLITKYNVKNFHFEDDNLLFDSKRFKDILRGIKGKRWNITWDTPNG
                     VRADLIDEEFLQLCRDTQCAYLMFGIESGSKKVLDKIIKKDIDLKDIERACKLCYDYE
                     IDTLGFFIFGLPGETKQDLLKTYYFAFDLFKKYSTTPIFQLWRPYKNTEMETNIRETE
                     NISQPVIFSLHQNFEIPYSLFYSQVYEDDEITIEFLSYYFKKYIRDGAKYAFLNWVKI
                     SRRKPLIFAHTVIQIIIIFSKSILSPKRLKYRLQQYMVSPGILPFAQLHRIGYKKHKH
                     "
BASE COUNT          876 a          511 c          507 g         1006 t
ORIGIN      
        1 ctgttgggca tgcgtcagca tcaacataga tagtcatgat tttcgtacca gctttcgggc
       61 aagtttatca acttctctgt taatatttct ctttgctgtt tttttctgat gagcagctac
      121 tttagataag cgcggcgaaa gtttttgaat cagcaaaaaa aaagtttcaa gtagctccct
      181 attctttctc tccgttcctt tgccggttat aaaatcactc gcaataagcg catccattct
      241 tgttacagct ccaacaacat actgactatc cgaatagagg acaagttctt gaagagggct
      301 catcttctcg acatactcca gagccttaat cgccgccaga agttccatgg cattatgcga
      361 gctggtcatt ttggttccgc ttaaaactat ctcttcacca aacataagaa tgattgcggc
      421 ccaggcaccg agcttcttgg caggattaca gctgccgtct gtatagattt ctataattaa
      481 attctcctgt acaccttaat atcaattact attctacttg atatttatat cgattattat
      541 tattctatag gtaatatttt catagcattt tttaaagaga gatataaaaa aattgattca
      601 tatgtataat tataaaagat attactgcat gtggctcttg ttactgtcgc tgctgcttgc
      661 ctcttgcagc agttacagaa atatccggca taattacatc tcattttcga agcatccggt
      721 ccaagggctt gatgtttccc gtcaccaggg tgagatagac tgggccaagg tgagcattag
      781 taaatttaca tttgtcttta taaaagccac cgaaggggga gacctggttg ataaaaaatt
      841 tgcctataac ttcaatcagg cccagcaaca tggtctcatt gttggggcct atcatttttt
      901 ttcatttaaa agaacaggca aagagcaggc caagaacttt atctcgacag ttccaaatat
      961 tccaggtatg ctgccgccag ttattgacct tgaatatatg ggtcgaagca atgaaaaaga
     1021 ttcaaaagaa aatcttcacc gtgaactgga tatatacatt gatgaaattg aaaaagcata
     1081 taatgtaaag ccaatcattt atacaaacta cagattttac agacgatatt tggaaggtca
     1141 ttatatcgaa tatgccatct ggatatgcga ttactataaa ctcccccaac tgccggatgg
     1201 gcgtccgtgg ctcttctggc agtattcaga tcgtggaaaa gttgccggta ttagagggtt
     1261 tgttgacttc aatgttttca atggtgaaaa agaagaactg atgaattttg taaagtaggg
     1321 tttctacttc tttcattatg gtaattacaa tataatatgg ttctcgtttt atcaatgctt
     1381 atgcttctta tatccaattc tgtgtaattg cgcgaacgga agtattccag ggcttaccat
     1441 atattgctgc agcctgtatt ttaaacgttt gggagaaagt atagatttgg aaaatataat
     1501 aattatctga ataactgtat gagcaaatat aagtggtttt cgtctggata ttttaaccca
     1561 gtttaaaaag gcatatttag caccgtcacg aatatatttt ttaaaataat aggataaaaa
     1621 ttcgattgta atttcatcgt cttcataaac ttggctgtaa aaaaggctgt atggtatttc
     1681 aaaattttga tgaagtgaga atataaccgg ttgtgaaata ttttctgttt cgcgtatgtt
     1741 tgtttccatt tcggtatttt tatacggtcg ccataattga aaaattgggg tagtgctgta
     1801 ttttttaaaa aggtcaaaag caaaatagta tgttttaagc agatcttgtt ttgtttcacc
     1861 aggtaatcca aaaataaaaa aaccaagtgt atcaatctcg taatcgtaac aaagtttaca
     1921 ggctctttcg atatctttta aatcaatatc tttttttatt atcttatcaa gaaccttctt
     1981 gcttccgctt tctattccga acatcaggta agcacattga gtatctcggc aaagttgtaa
     2041 aaattcttca tcaattagat cggctctgac accatttggt gtatcccagg tgatgttcca
     2101 acgctttccc tttattccac gaagaatatc cttaaaacgc tttgagtcaa acaaaagatt
     2161 gtcgtcttca aagtgaaaat ttttaacatt atattttgtt attaagagct ccatttcatc
     2221 caaaacatat tgaacactat gggctcgaac ttttttgccc atgtgaagat gtatacagca
     2281 gaaactgcag ttcatctgac agcctcggct tgtaacaatg tcaattgatc ggtgtgatcc
     2341 tggtgattta taaatcaaac gagatggaaa cccttctttg ttaatattaa aatatttttc
     2401 aagatcaagt acttcgtaat cacatttata tctgtcaaga tcatcaatgt atggtctttg
     2461 agggttaatt tttattttcc catttttttt atatgcaata cccttgatgc caaatattga
     2521 ttttgaatta attagcgatt caatgatttc tctaaatgtc tcttcgcctt ctctcattat
     2581 tacggcagta attccaggtg ctttttctaa atacatctgg ttgtcatttg ttgcgtccgg
     2641 gccaccgatt agaataatga tatctggaaa attacgatgt atcagattga ttattttaat
     2701 tgtatctttt gcaaaataat ttgccgtaca tgtaattgcg atacatgccg gagagtatga
     2761 ttttacataa tcaataattt ccgaaacttt catgccaaaa tagataggag gttttgtttt
     2821 attgtctcta acatgtttaa aattaggatt cgcaagtata tctaaaaaat cagtttcgat
     2881 attattcata tctttaattg
//
LOCUS       DAOTGZ010000297         2816 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_3064
            NODE_42297_length_2816_cov_6.027888, whole genome shotgun sequence.
ACCESSION   DAOTGZ010000297 DAOTGZ010000000
VERSION     DAOTGZ010000297.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868088
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 2816)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2816)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:22:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,785
            CDSs (total)                      :: 2,751
            Genes (coding)                    :: 2,732
            CDSs (with protein)               :: 2,732
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 12 of 19
            Pseudo Genes (internal stop)      :: 5 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2816
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_42297_length_2816_cov_6.027888"
                     /isolate="AMR_MDS_3064"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..264)
                     /locus_tag="P5123_13105"
     CDS             complement(<1..264)
                     /locus_tag="P5123_13105"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRX17248.1"
                     /translation="MKFVKSDLLWLSICVANAVVGALYGTLCGFHKGAMLIGVSVFVV
                     IFLSLSHTGFLDRLKTSTVLYRSVIIGFFVNALLFPIHVILGIS"
     gene            complement(304..699)
                     /locus_tag="P5123_13110"
     CDS             complement(304..699)
                     /locus_tag="P5123_13110"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRX17249.1"
                     /translation="MLPQYLIARWKGINVLPLPLGRSYLDRFGPNYEAYDNIAGPLYQ
                     ALKKEPNDFNKMWKEAVKLTRERNTEQECMRKMEQALRNECSFLQKAESLLVVETGLQ
                     ATMPLYFYTISFSFHSEFILFSQSLFIVN"
     gene            1200..1454
                     /locus_tag="P5123_13115"
     CDS             1200..1454
                     /locus_tag="P5123_13115"
                     /inference="COORDINATES: protein motif:HMM:NF025120.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HRX17250.1"
                     /translation="MRSITKQQITSIEILGKTIKSERNRRGLTQTELGNITGVKQKTI
                     SSAENGSSGLKLDTLFRILAGLNMSISLNVHEKEKTTGKW"
     gene            1454..2758
                     /locus_tag="P5123_13120"
     CDS             1454..2758
                     /locus_tag="P5123_13120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019233310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system HipA family
                     toxin"
                     /protein_id="HRX17251.1"
                     /translation="MRGSQKTSMLDMYMNGEKIGILHRDSSGRLAIVYAEEWLESEKC
                     RPLSLSLPVTQKRHNGDVVNNFFDNLLPDSRQIINRIQTRFTIPSDNSFDILSYIGQD
                     CIGAIQLLPFNSTAPDIKKINVQTVSDKQIAEILKNYQVMPLGMNSTDNFRISISGAQ
                     EKTGLLRYKGKWCRPLGPTPTSHIFKLPIGMITQSGIDLSESVENEWLCHLLLKEFDI
                     PTADMDIFKYDNVKALIVKRFDRVWSDDKSWLIRLPQEDLCQAKGLSSAFKYESDGGP
                     GIKDILQILQGSSDMQADSKIFMKTVFVFWLLGAIDGHAKNFSIFINPGGSFRLTPVY
                     DVLSAYPLISQKQLPKQKLKMAMSVRGKSVHYKWYNIRLQHWMSNAKYCRFSENIMSE
                     IIDTTVSSIDKAIANVSSKLPLNFPRKISDPIFEFLLKMKYR"
BASE COUNT          977 a          561 c          515 g          763 t
ORIGIN      
        1 agagatgcct aaaataacat gtattggaaa cagtaacgcg tttacaaaaa aaccgattat
       61 tactgatcgg tacaaaacag tacttgtttt taatcgatct aaaaaaccgg tatgagataa
      121 gctcaaaaag atcacaacaa atacagatac tccaattagc attgcccctt tatggaagcc
      181 gcataaagtt ccgtataatg ctccaacaac agcatttgct acgcagattg ataaccacaa
      241 taaatcgctt ttcacaaatt tcataatcca cacctctttt tttaatgtcc tttaattaac
      301 cttttaatta acgataaaaa gcgactgtga aaacagtatg aactctgagt gaaaagaaaa
      361 agaaatcgta taaaaataaa gaggcatcgt cgcctgaaga cctgtctcga ccacaagcaa
      421 agattctgct ttttgtaaaa aggagcattc attacgcaaa gcctgctcca tttttctcat
      481 acactcttgc tcggtattgc gttcacgtgt caacttgaca gcttctttcc acattttgtt
      541 gaaatcatta ggctcttttt ttaaagcctg ataaagcgga ccggctatat tatcgtatgc
      601 ttcatagttt ggaccaaaac ggtcaagata tgagcgtcca agaggaaggg gcagcacatt
      661 aatgcctttc cagcgagcta tcaggtattg gggaagcata tcacgcagca acgcgacaaa
      721 ggcgagaagg tcaattcaga agagatctca aaagcacatg ccgagatcat gagtcgcatt
      781 atggggctga taccaggcag atagctgtcc gttaagtaaa tcatcttatt attataaaaa
      841 tgttcaacct catcccggag caggcggatt tatccttatt ctatgttttc gaattatcta
      901 caagtacaaa tacaatattt catatggcta ttgaagtatg actaaacccg aacggttcct
      961 gacaaaagca cctttgccaa tatatagcaa ctgcaaacag aaaggtaacc attcaatatc
     1021 taccattcaa aaaatataaa ctattacaga aatactgatt aagcaggcaa ataacaggaa
     1081 aatacaaagt cggtgatgtt aacaaaaacc tgttgacaac agtatacccc aagagttata
     1141 tctacctatt attccttgta agggataatt gccaaatata ccctgtatgg aataatcaaa
     1201 tgagatctat aacgaaacaa cagataactt cgatcgaaat tcttggcaaa accataaaat
     1261 ccgaacggaa cagaagaggc cttactcaaa ccgaactggg aaacatcacg ggagtaaaac
     1321 aaaaaaccat atccagtgcg gaaaatggat cgtcgggttt aaaactcgat accctctttc
     1381 gtattcttgc cgggctcaat atgagcatat cactcaatgt tcacgaaaaa gagaaaacaa
     1441 cgggcaagtg gtaatgaggg gttcacaaaa aacatcaatg ctcgatatgt atatgaatgg
     1501 agaaaaaata ggcatattgc accgtgattc gtcgggaagg cttgcgattg tatatgctga
     1561 agagtggctt gagtcagaaa aatgccgtcc tttatctttg tctttgccgg ttacacaaaa
     1621 acggcacaac ggagatgttg ttaacaactt ttttgacaat cttttacctg acagccgtca
     1681 gatcatcaac agaatacaaa cacgttttac catcccttcc gacaacagtt tcgatattct
     1741 ttcatatatc ggtcaagatt gtattggagc aattcaattg ctccctttta attctactgc
     1801 acccgatata aaaaaaataa atgtacaaac agtttcagat aaacaaattg cagaaatact
     1861 aaaaaactat caagtaatgc cgctcggaat gaacagcaca gacaattttc gcatttccat
     1921 atccggtgct caggaaaaaa ctgggttact acgttacaaa ggcaaatggt gtcgaccatt
     1981 aggtcccact ccgacaagtc atatcttcaa actacctata ggaatgatca cgcaaagtgg
     2041 tattgaccta tcggaaagtg tggaaaacga gtggctctgt catttactac taaaagaatt
     2101 tgacatcccg acagcggata tggatatctt taagtatgat aacgtgaaag cacttatagt
     2161 caaacgattt gaccgtgtat ggagcgatga taaatcttgg ctaatcagac tgccacagga
     2221 agatttatgt caggcaaaag gattatcttc ggcttttaaa tacgaaagtg atggcggtcc
     2281 cggcattaaa gatatcctgc aaattcttca ggggtcttct gatatgcaag ccgatagtaa
     2341 aatatttatg aaaacagtat ttgttttctg gcttctcgga gctattgacg gacacgcaaa
     2401 aaatttcagc atatttatta atccgggtgg ctcattccgg ttaaccccgg tatatgatgt
     2461 tctgtccgcc tatcctctta tttcacaaaa gcaacttcct aagcaaaaac taaaaatggc
     2521 catgtcagtc agaggaaaat cggttcatta taaatggtac aatatccggc tgcagcattg
     2581 gatgagtaat gccaaatatt gccggttttc cgaaaacata atgtcagaaa ttattgacac
     2641 tacagtaagc tctattgaca aagcaatagc aaatgtttca tcaaaactac cgctaaattt
     2701 tcctcgaaaa atatctgatc caatttttga atttttgttg aaaatgaaat atcgataagt
     2761 ctgccataac cttcgcgttt gaaaaacaga ctatgggtgg ctaacaatcc aggaaa
//
LOCUS       DAOTGZ010000298         2808 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_3064
            NODE_42455_length_2808_cov_4.386487, whole genome shotgun sequence.
ACCESSION   DAOTGZ010000298 DAOTGZ010000000
VERSION     DAOTGZ010000298.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868088
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 2808)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2808)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:22:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,785
            CDSs (total)                      :: 2,751
            Genes (coding)                    :: 2,732
            CDSs (with protein)               :: 2,732
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 12 of 19
            Pseudo Genes (internal stop)      :: 5 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2808
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_42455_length_2808_cov_4.386487"
                     /isolate="AMR_MDS_3064"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            351..2330
                     /locus_tag="P5123_13125"
     CDS             351..2330
                     /locus_tag="P5123_13125"
                     /inference="COORDINATES: protein motif:HMM:NF024760.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic nucleotide-binding domain-containing
                     protein"
                     /protein_id="HRX17252.1"
                     /translation="MHDLALWKNILIALSPVVVFYLIYARYSSSYPDLSKQLQAFIAG
                     IIAALLIIVIKPYVILLVPSGNLWISAFIKAAFLEKIFVFFLLLAVFRHYPDFTLLEA
                     SLSGILLGIGFSVCENIFYALSYGTNTMIIRIIFTVPLHMTTCGIMGYYIGESLYSGS
                     KIYKSYYFTLSLLVPVFFHGLFDYVLLHQNHYVFFASPLLLIVVIIQEAVLSRSTTVP
                     GLEVMNALALYYEEWNVLSRQPKYDRWMQKSMGRKHTPKVNFFVWRPGAFQAMMIIGF
                     IVLSVIGSWYRKDIGFYFLLTGWEENLLLIIFPLSIAATIVIVNAVNPEFFKYNELKI
                     PIISNAVIEVEDGIEDNHITYDMTAVNVFINTFEPLGVGNKYTLDIEVPHMRSRTIDG
                     TVIWEKHVIGHSPAGSLIRFNNITPGFAYFIIFRYYVSRFIKGIIFNFHLPGFESIKS
                     FFIHPVSTMRDEKIYKAGELIYGEGENSQSFFFLKKGIVEIYKSHQDEEIPLNILNDG
                     SIFGEMSVFGGARRTHTARALTDCLIAVSDKENLHILVKNNTEFALRLFESLSIRITE
                     TENSLYNHIRDLDMKKKENQKLFDSLSMILISTLRDNKDISPELLEFSRLKAIVENIN
                     DEIASKLVSILTCHGQKDIEKIEDSLRYIYSVVEK"
     gene            2386..>2808
                     /locus_tag="P5123_13130"
     CDS             2386..>2808
                     /locus_tag="P5123_13130"
                     /inference="COORDINATES: protein motif:HMM:NF012376.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HRX17253.1"
                     /translation="MKILLLGDKESTYIWDYFDPERFKGVDFVISSGDLKREYLSFVV
                     TMLNVPLYFVPGNHDKNYVELEPEGCVNIDRKIVDHSGFKIMGLGGSQWYNDGVYQYT
                     EKQMYFDYLKLKPKILFNKGIDMLVTHSPALGLNDGNDR"
BASE COUNT          831 a          507 c          557 g          913 t
ORIGIN      
        1 tcaaaataaa gcaaggtgcc gaaaaatggt gatatattca ccaagaacta attaacttta
       61 taacacggat gtatagacga gcagcccagc accattatta aatgagaaaa attgtcttta
      121 atatacatta agcagcatag ccgctccgga aatggtaatt tttgaagtcc cctactgtta
      181 aaacataatg ttctgttata ggtcataatt ttatgttttt ttacagtaaa acagttgccg
      241 atattcagag tgtggaataa aatatgtcat tcttccaaat atctgatcac ttaattttgt
      301 aatgaattga tgtttcgagg ttaacggtat gtccttaaag agagtttacc atgcatgatc
      361 tggccttatg gaagaatatt cttatcgctc tttcaccagt tgttgttttt tatcttattt
      421 acgcacgtta ctcctcttca tatcccgatt tatcgaaaca gcttcaagcg tttattgccg
      481 gtatcatagc cgccctatta attattgtca ttaaacctta tgtaatttta cttgttcctt
      541 cgggtaatct ttggataagc gcttttatta aagcggcttt tctggaaaaa atatttgtct
      601 tttttcttct tttggctgtg ttcaggcact atcccgattt tacactgctc gaagcttctc
      661 taagcggcat tcttttgggt ataggctttt cggtttgcga gaatatcttc tatgctttaa
      721 gttacggaac taataccatg attatccgaa tcatatttac ggttcctctt catatgacaa
      781 cctgtggtat aatgggatac tatatcggag agtctctcta ttccggttcg aaaatatata
      841 aatcttacta ttttacgtta agccttcttg tcccggtttt ttttcacggc ctgttcgatt
      901 atgttttact tcatcagaac cattatgttt tttttgcgtc tccgcttctg cttattgtag
      961 tgattattca ggaggctgtc ctgtccaggt ccacaaccgt tccggggctc gaagttatga
     1021 acgcccttgc cttatattac gaggagtgga acgtgctaag ccgtcaaccc aagtacgaca
     1081 ggtggatgca gaaatccatg ggacgaaaac acactccaaa ggtaaacttc tttgtctggc
     1141 ggccgggcgc ctttcaggct atgatgataa taggatttat tgttctgtcg gtaatagggt
     1201 cgtggtaccg gaaggatatc ggcttctatt tccttttaac aggctgggag gaaaatctgc
     1261 ttctgataat atttccgctc tctatcgcgg caaccattgt aattgttaac gcagttaacc
     1321 ctgagttttt caaatacaac gaattgaaga tacctataat atcgaatgct gttattgaag
     1381 tcgaagacgg catagaggat aaccatatta catacgatat gacagcagta aacgttttca
     1441 taaacacttt cgaaccattg ggggtaggca acaaatatac acttgatatt gaagtacccc
     1501 acatgagatc ccgcaccata gatggtacag tcatttggga aaaacatgtg attggccaca
     1561 gccccgccgg ttctctaatc cgttttaata atataacacc aggatttgcc tattttataa
     1621 ttttcagata ttacgtatca aggtttataa agggcataat ttttaatttt catcttccag
     1681 ggtttgagag cataaagagt ttttttatac atcccgtctc gacaatgcgg gacgagaaga
     1741 tatataaggc cggagagctt atatacggtg aaggcgaaaa ctctcagagt ttcttctttt
     1801 taaaaaaggg tatagtcgaa atttacaaga gtcatcaaga tgaagagatt ccgcttaata
     1861 tacttaatga cggctctatt tttggtgaaa tgtctgtttt cggtggggca aggcgaactc
     1921 atacggcgcg agcgctgact gactgcctga tcgctgtatc agacaaggag aatttacata
     1981 ttctggtaaa aaacaatacc gaatttgcgc ttcgtctttt cgaatctctc tcaatcagaa
     2041 taacagagac agaaaattcg ctatataatc atatcaggga tctggacatg aaaaaaaagg
     2101 agaatcaaaa attgttcgat tctttgtcaa tgattctgat atcaactctt cgagacaaca
     2161 aggatatctc tcctgagctt ctcgaattct cccgcttaaa agcaattgtt gaaaatataa
     2221 atgacgagat cgcttccaaa cttgtcagca ttcttacgtg tcatgggcaa aaggatatag
     2281 aaaagattga agatagtctt cgttatatct attctgttgt agagaagtga aaattggttg
     2341 ataataaaga ccgccctatt aggttgtcca acaggaaaga taattatgaa gatactgttg
     2401 cttggagata aagaatctac atatatatgg gactactttg atcctgaaag gttcaaggga
     2461 gtcgatttcg ttatttccag tggagattta aagcgggagt atctctcatt tgttgtaacc
     2521 atgcttaatg tccctctata ttttgttcct ggtaatcatg ataagaatta tgtagaactc
     2581 gagcccgaag gatgcgtaaa catcgacaga aaaatagtgg atcatagtgg ctttaagata
     2641 atggggcttg gtggttcaca gtggtataat gacggtgtgt atcaatacac ggaaaagcag
     2701 atgtattttg attatttaaa attaaaacca aaaatacttt ttaataaagg tattgatatg
     2761 cttgttacac attctccagc attgggttta aatgacggca atgatcgt
//
LOCUS       DAOTGZ010000299         2803 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_3064
            NODE_42562_length_2803_cov_4.909389, whole genome shotgun sequence.
ACCESSION   DAOTGZ010000299 DAOTGZ010000000
VERSION     DAOTGZ010000299.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868088
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 2803)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2803)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:22:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,785
            CDSs (total)                      :: 2,751
            Genes (coding)                    :: 2,732
            CDSs (with protein)               :: 2,732
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 12 of 19
            Pseudo Genes (internal stop)      :: 5 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2803
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_42562_length_2803_cov_4.909389"
                     /isolate="AMR_MDS_3064"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..305)
                     /locus_tag="P5123_13135"
     CDS             complement(<1..305)
                     /locus_tag="P5123_13135"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRX17254.1"
                     /translation="MKKRIIKNRITLGEAQPRVVVNENFSDFTPSTDRKKHVIQIVTI
                     VTIFLAGITAAFYFIFLHNPKKLDQEPGRTALEDVNWDTVSTPSETGSSLNNPNLLTG
                     "
     gene            complement(406..810)
                     /gene="nusB"
                     /locus_tag="P5123_13140"
     CDS             complement(406..810)
                     /gene="nusB"
                     /locus_tag="P5123_13140"
                     /inference="COORDINATES: protein motif:HMM:TIGR01951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antitermination factor NusB"
                     /protein_id="HRX17255.1"
                     /translation="MGKRRKSREIALQALYMIDSVESSVDQICSFDWLNELYDQDTLN
                     FSEKIIRGTLHKIDQIDDIIKRYAKNWKFDRIERIDKTILRMSIFSLQELTEIPAAVT
                     INEAIELGKIYGGENSGTFINGILDSVRKHEL"
     gene            complement(816..1277)
                     /gene="ribE"
                     /locus_tag="P5123_13145"
     CDS             complement(816..1277)
                     /gene="ribE"
                     /locus_tag="P5123_13145"
                     /EC_number="2.5.1.78"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005902111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009349 - riboflavin synthase complex
                     [Evidence IEA];
                     GO_function: GO:0000906 - 6,7-dimethyl-8-ribityllumazine
                     synthase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6,7-dimethyl-8-ribityllumazine synthase"
                     /protein_id="HRX17256.1"
                     /translation="MKVFEGNLNASGLKIAIAASRFNEFITSKLIGGALDCLKRHECE
                     EENISLAWVPGAFELPSAAARLAKSKKYDAVICLGTIIRGSTPHFDYVAAEASKGIAH
                     VSAETDVPVIFGVLTTDSIEQAVERAGTKAGNKGFDAAMSAIEMANLYKKI"
     gene            complement(1417..2265)
                     /gene="ispH"
                     /locus_tag="P5123_13150"
     CDS             complement(1417..2265)
                     /gene="ispH"
                     /locus_tag="P5123_13150"
                     /EC_number="1.17.7.4"
                     /inference="COORDINATES: protein motif:HMM:TIGR00216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051745 - 4-hydroxy-3-methylbut-2-en-1-yl
                     diphosphate reductase activity [Evidence IEA];
                     GO_process: GO:0009240 - isopentenyl diphosphate
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0015968 - stringent response [Evidence
                     IEA];
                     GO_process: GO:0046677 - response to antibiotic [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-3-methylbut-2-enyl diphosphate
                     reductase"
                     /protein_id="HRX17257.1"
                     /translation="MKLIVDNLAGFCMGVRNAVEKVTTELEKQPLIYIHGDIIHNPQT
                     ISILKKKGLISYKDFNEVPAKSCVAIRTHGIPYNEYVSIKNHASSIVNLCCSRVTKIQ
                     SIIKKAGLTNSHVIICGYHDHPEVKSLQSYASNGVTVIKHPDEIDLVPLTDKLLLISQ
                     TTFDEELFYKISDAAKIKFGNRIDIINTICRATHDRQKNLISLLTKNQVTKLIVIGGR
                     QSSNTKRLADIGKSRKIATYHIEELAEFDLNTVVTDDTIVVTAGASTPDYIINEVINA
                     LNELQK"
     gene            complement(2265..>2803)
                     /gene="gspN"
                     /locus_tag="P5123_13155"
     CDS             complement(2265..>2803)
                     /gene="gspN"
                     /locus_tag="P5123_13155"
                     /inference="COORDINATES: protein motif:HMM:TIGR04411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="type II secretion system protein GspN"
                     /protein_id="HRX17258.1"
                     /translation="KLSYTNTNLFSIMLFKDLKTDFSFSNMTFESNGKSYGGSLSGTT
                     DLELNSQNYMPANGNIRLSLKNLTLSGLKITGFDIPDIKINQLLSEIEFKDLTATFSK
                     TAFNGEDLNGSISGTITLDDKNTNNSRINLIIDIRQDSGLIEKFRMLIASYINPATNT
                     IRISVGGTFSRPDIKFNR"
BASE COUNT          779 a          504 c          529 g          991 t
ORIGIN      
        1 cctgttagta ggttcggatt gtttaaagag cttccagttt cagaaggtgt ggaaacggta
       61 tcccagttta catcttcaag agctgttctt ccgggttcct gatcgagttt ttttggatta
      121 tgtaaaaaaa tgaagtaaaa agccgcggtt atacctgcaa gaaaaatcgt tactattgta
      181 acgatctgta ttacatgttt tttccgatct gttgaaggag taaaatcaga aaaattttca
      241 tttacaacaa cgcggggctg tgcttctccg agggtgattc tgtttttaat gattcttttt
      301 ttcataataa cctgactgat attattagct cttttttaac cagccggctt tattgccata
      361 tgccggcgga ctgttgttaa acagcaccga caatggtact gttttttaca gctcgtgttt
      421 tctgaccgaa tccaggatac cgttaataaa ggttccggaa ttttcgccgc cgtaaatctt
      481 tccgagttct atagcctcat taattgtaac ggctgcaggt atttcagtca actcctgtaa
      541 agagaatatc gacatcctta aaatggtctt gtcaatcctc tcgatacggt caaatttcca
      601 gtttttagcg tatctcttta taatatcgtc aatttgatca attttatgca gtgttcctct
      661 tataatcttt tcagaaaaat tgagtgtatc ttgatcgtaa agctcgttta accaatcaaa
      721 agagcatatt tgatcaacag aactttcgac agaatcgatc atatagagag cctgcaaggc
      781 tatttctcgt gattttcttc gtttacccat ctgatttata tctttttata gagatttgcc
      841 atctctatag cgctcattgc ggcgtcaaaa cccttgtttc ctgcctttgt accggctctt
      901 tccacagctt gttcgataga atctgttgta agtacgccaa aaattacagg tacatcagtt
      961 tctgccgaaa cgtgtgcgat tcccttagag gcttcagcgg caacataatc aaaatgtggg
     1021 gttgatccgc gaataattgt acccaaacag attacagcgt catatttttt cgattttgca
     1081 agccgtgccg cggctgatgg cagttcaaaa gcaccgggaa cccaagccag ggatatattt
     1141 tcttcttcgc attcatgccg tttaaggcag tcaagcgcac cgcctattaa cttcgatgta
     1201 ataaattcgt taaatcgtga tgcagctatt gctattttga gaccggaggc gtttaagtta
     1261 ccttcaaata ctttcattgt tttacccctg tttaaaaaaa ttattgggac gttttacgtc
     1321 attgaagaaa aaaaagattt ccttgtcaat agtgatgtaa aaaaagataa aaattaaatc
     1381 gataagaata gtgattagtt cggctgttat atctttctat ttttgaagtt catttaaagc
     1441 gtttattact tcatttatta tatagtccgg tgttgatgct ccagctgtca ctactatagt
     1501 gtcatcagtt actactgtat taagatcaaa ttcggcaagc tcttcaatat gatatgtggc
     1561 tatttttcgt gatttgccaa tatcagcaag ccgtttggtg ttgcttgatt ggcgaccgcc
     1621 gattacaatc agtttagtta cctggttttt tgttaaaagc gatataaggt tcttttggcg
     1681 atcgtgtgtt gctctgcaaa tagtgttgat aatatcaatg cgattgccga atttaatttt
     1741 tgccgcatca gatattttat aaaatagttc ttcatcaaag gttgtttggg aaattagtag
     1801 tagtttatct gtcagaggaa ccaggtctat ttcatcaggg tgtttgatta ctgttactcc
     1861 gtttgacgcg taactctgta aagatttaac ctccggatgg tcgtgataac cacagataat
     1921 aacatgagaa tttgttaaac ccgccttctt aattatagat tgtatttttg ttactcttga
     1981 acaacagagg ttaacaatag aggatgcgtg attttttata gaaacatact cattataagg
     2041 tatgccatgt gtgcgtattg caacacagct tttcgctgga acttcattga agtctttgta
     2101 gctaataagc ccctttttct ttagaatact aatggtttga ggattatgga ttatatcgcc
     2161 gtgtatataa attagaggct gcttttccag ttcggtggtt actttttcta ctgcattgcg
     2221 tacccccata caaaaccctg ccagattatc aactatgagt ttcattatcg gttaaacttt
     2281 atatcgggac gtgaaaaagt accaccaaca ctaattctta tcgtatttgt cgctgggttt
     2341 atataacttg cgatcaacat acggaatttt tcaattagcc ctgaatcctg tcgaatatca
     2401 attatcaaat taattcggga attgttagta tttttatcat ctaaggttat tgttcctgaa
     2461 atgcttccat taagatcttc gccgttgaat gctgttttag aaaaagttgc cgtaaggtct
     2521 ttaaattcaa tttcacttaa aagctgattt attttaatat ccggtatgtc aaagccggtt
     2581 attttaagac cgcttagggt aaggtttttt agggacaatc ttatattgcc gtttgcaggc
     2641 atatagtttt ggctgttaag ttcaagatct gtggttccgc taagtgatcc accatatgat
     2701 ttgccattac tttcaaaggt catatttgaa aaagaaaaat cagttttaag gtctttaaaa
     2761 agcataatcg aaaacagatt cgtgtttgta taagataatt ttg
//
LOCUS       DAOTGZ010000300         2802 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_3064
            NODE_42583_length_2802_cov_3.728067, whole genome shotgun sequence.
ACCESSION   DAOTGZ010000300 DAOTGZ010000000
VERSION     DAOTGZ010000300.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868088
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 2802)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2802)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:22:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,785
            CDSs (total)                      :: 2,751
            Genes (coding)                    :: 2,732
            CDSs (with protein)               :: 2,732
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 12 of 19
            Pseudo Genes (internal stop)      :: 5 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2802
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_42583_length_2802_cov_3.728067"
                     /isolate="AMR_MDS_3064"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..894
                     /locus_tag="P5123_13160"
     CDS             <1..894
                     /locus_tag="P5123_13160"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HRX17259.1"
                     /translation="GIPRKGYEAQSLSFLCEEAGVDPDDIFSRLKSKTKTADKIEQEI
                     NYLTIEPGTNKTGKKEFKENIEICKGEVIALVGPTGAGKSLMLSDIEGMAAGDSPSSR
                     IIKINGKNYDEFYQGKNIKSPIALISQSMNYLIDLKVNEFLDIHMECCEIAEKHLQKK
                     VVDEACHLCGESFSLDTRMVSLSGGQARALMISDSLNITRAPVILVDEIENAGIRKDK
                     AVELLVNSSSISIIATHDPFIALLANRRIILQNGGISSVIERSESEKETVKILKKINE
                     YSENLKDKLRFGKILDINFEL"
     gene            complement(1005..1730)
                     /locus_tag="P5123_13165"
     CDS             complement(1005..1730)
                     /locus_tag="P5123_13165"
                     /inference="COORDINATES: protein motif:HMM:NF013080.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoethanolamine transferase"
                     /protein_id="HRX17260.1"
                     /translation="MKEAGYTTYFLNNNSHDISLIHYYIKESDNYINTEKPMGPDDKV
                     VPLFEKILKDKSRKKFIIIHLYGSHFIYNERYPSSFSMYKPDRYNRITYANKKEIINS
                     YDNTILYTDYVINSLITELKKTGNSSSLVFFSDHGENLFDTDEKLKFHVNFNKYTYRI
                     PFLIWFSDDFINRREVGLTLETNSKKLTDTKCLFHTVMDIAGMDSKFFDTSKSLLDPN
                     FKAQDSVHYYDNNNEPVAIAYEE"
     gene            1830..2045
                     /locus_tag="P5123_13170"
     CDS             1830..2045
                     /locus_tag="P5123_13170"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRX17261.1"
                     /translation="MYPKQKHLRKEEIKLIHLTMITKPVEEIAKSILKMHMIEKRKGW
                     KEVNQKRVVNRINIKFKEEEYGLVGST"
     gene            2023..2715
                     /locus_tag="P5123_13175"
     CDS             2023..2715
                     /locus_tag="P5123_13175"
                     /inference="COORDINATES: protein motif:HMM:NF026766.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Imm26 family immunity protein"
                     /protein_id="HRX17262.1"
                     /translation="MGWWEAPEDKKIVVGDDVLDMVRHFLKDFSNSYQNSLSRKPSVD
                     ELQYMLNLGFSVNADDEILSDFEEKEIKQVNIKVAKRAKRQKAKQGDIFCFKIDDKRF
                     GFGRIVSEVMLGWVAEIFDYFSDKPVFDYNKLGKWLIDPIILDCYTLFELKLEGDWKI
                     IGSNPGFNPGEEFKNVRFVTGDGLGGNLWSVDIFDNKTRITKEESKGLRRFSACGNYD
                     VLQLVSEALKNR"
BASE COUNT          956 a          379 c          593 g          874 t
ORIGIN      
        1 ggtatccccc gcaagggtta cgaggcacag tcactttctt ttttatgtga ggaagccgga
       61 gtagatccgg atgatatatt ttctagactt aagagcaaaa ccaaaacagc agacaagatt
      121 gagcaagaga taaattatct gacaatagag cctggaacaa ataaaactgg taaaaaagaa
      181 tttaaagaaa acatcgaaat ttgcaaagga gaggtgatcg ctcttgttgg cccaaccggg
      241 gccggtaagt ctctcatgct ttcagatatc gagggaatgg ctgccggaga ctctccttct
      301 tcaagaataa taaaaataaa tgggaaaaac tatgatgagt tttatcaggg gaaaaatata
      361 aagagcccga ttgctcttat ttcacagagc atgaattatc tgattgatct taaggtaaat
      421 gagtttcttg atatacatat ggagtgctgt gaaattgcgg agaaacattt gcaaaaaaaa
      481 gtagtggatg aggcttgcca cctttgtggt gaatcttttt ctttagatac aaggatggtg
      541 tctctgtcgg gcgggcaggc tcgagctctt atgatctctg attctttaaa tattacaaga
      601 gctccggtta ttctggtaga tgagatcgaa aacgccggaa ttagaaaaga caaggccgtg
      661 gagcttttag taaactcaag ttctatcagc ataatcgcga ctcacgatcc atttattgcg
      721 ttattggcaa accgaagaat cattttacaa aacggtggta tttcttctgt tatcgaaaga
      781 tcggaatctg aaaaagaaac agtaaaaata ttgaaaaaga ttaatgaata ttctgaaaat
      841 ttaaaagata aactgagatt cggtaaaata ttagatataa attttgaatt gtaaactctt
      901 aataagctaa cttctaaaaa atattttttt acgctcagat atagcgtttt tcttagagtg
      961 tgttatacct gatcggtttt ccagtgataa caaaacagtt tggattactc ctcgtaagca
     1021 atcgcgacgg gttcgttgtt gttgtcataa tagtgcaccg aatcctgggc tttaaaattg
     1081 gggtcaagta aactctttga tgtatcgaaa aatttagaat ccattccggc gatatccatt
     1141 acagtgtgaa aaaggcattt tgtgtctgtt agttttttac tgttggtctc aagagttaat
     1201 ccaacttcac gtctgttgat gaaatcgtct gaaaaccaga taagaaacgg tattctatat
     1261 gtatatttat tgaaattgac atgaaactta agtttttcgt cagtatcaaa aaggttctct
     1321 ccatgatctg aaaagaaaac caggcttgat gaatttccgg ttttctttag ctcagttatt
     1381 agactattaa ttacataatc tgtatacaaa atggtgttgt cgtatgagtt aattatctct
     1441 tttttgttag cgtatgtaat tctgttatag cgatcaggtt tatacattga aaaacttgac
     1501 ggatatcttt cgttatatat aaaatgtgag ccgtacaaat ggatgatgat gaattttttg
     1561 cggcttttat ctttcaatat tttttcaaaa agcggaacca ccttgtcgtc aggacccatc
     1621 ggcttttcgg tatttatata attgtcgctc tcctttatat aataatgtat tagtgatatg
     1681 tcatgagaat tgttattcaa aaaataggtg gtatatccag cttctttcat agcggtaatg
     1741 atactctttt cggtatagag gtgaggcacg tggaatcgag caggcaaata tagagcatca
     1801 taaaacaaaa acagcagagg ttgggtcgga tgtatccaaa gcaaaaacat ctaaggaaag
     1861 aggaaataaa attaattcat ttgaccatga taacaaaacc cgtagaagag atcgccaaaa
     1921 gtattttgaa gatgcatatg atagagaaaa gaaaaggctg gaaggaagta aaccaaaaaa
     1981 gggttgtaaa tagaataaac ataaaattta aggaggaaga atatgggctg gtgggaagca
     2041 cctgaagata agaaaatagt tgtaggagac gatgttttgg atatggttcg tcattttttg
     2101 aaagacttta gtaattcata tcaaaatagt ctttcaagaa agccatctgt agatgagttg
     2161 cagtatatgc ttaacttagg gtttagcgtt aatgcggatg atgaaatact ttcagatttt
     2221 gaagaaaagg aaataaagca agttaatata aaggtggcaa aacgtgcaaa acgccaaaag
     2281 gctaaacaag gagacatttt ttgctttaag attgatgata aaaggttcgg atttggtcgt
     2341 atagtttctg aagtaatgtt aggctgggtt gctgaaatat ttgactattt ttcagataaa
     2401 ccggtatttg attataacaa attaggaaaa tggcttatag atccaattat tcttgattgt
     2461 tataccttat ttgaacttaa acttgaaggc gattggaaaa taattggaag taatcctggc
     2521 tttaatccag gagaagaatt taagaatgtt aggtttgtga ctggtgatgg cctaggaggg
     2581 aatttgtggt ctgttgatat ttttgacaat aaaacacgaa ttacaaaaga ggaatcaaag
     2641 gggttacgta gattttcagc ttgtggtaat tatgatgtgt tacaattggt ttcagaggct
     2701 ttaaaaaata gataaagttg ttttataagc tataaaaaat agattaagta aatcaaataa
     2761 ggtaattcca aaataatgag gcttgtgatg gtgtatttga ag
//
LOCUS       DAOTGZ010000301         2796 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_3064
            NODE_42705_length_2796_cov_3.221087, whole genome shotgun sequence.
ACCESSION   DAOTGZ010000301 DAOTGZ010000000
VERSION     DAOTGZ010000301.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868088
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 2796)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2796)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:22:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,785
            CDSs (total)                      :: 2,751
            Genes (coding)                    :: 2,732
            CDSs (with protein)               :: 2,732
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 12 of 19
            Pseudo Genes (internal stop)      :: 5 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2796
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_42705_length_2796_cov_3.221087"
                     /isolate="AMR_MDS_3064"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..572)
                     /locus_tag="P5123_13180"
     CDS             complement(<1..572)
                     /locus_tag="P5123_13180"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRX17263.1"
                     /translation="MNRLKIYSALIITALLLSMPTLSQEKVPGKVRQRKGDINSFSVY
                     LKMEKLKFYNSEDIILHVVIKNITDEVASFYVFDPPGREDMFNRERGDTADYNTFKPV
                     VYDMTGRNAELIVPYMASGSDLKETLSWMTRREIKLGPGESFTRSLNLGKMYRLQSDT
                     DYRVKLHFFPFTGDPDEEKISISDNELKVR"
     gene            complement(1021..2034)
                     /locus_tag="P5123_13185"
     CDS             complement(1021..2034)
                     /locus_tag="P5123_13185"
                     /inference="COORDINATES: protein motif:HMM:NF012307.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="reverse transcriptase/maturase family protein"
                     /protein_id="HRX17264.1"
                     /translation="MRRVGNLKETFLSFENIYSGYKKAFKATKNYSACEFTFNLEKEL
                     FLLKEELSSSEYIPRDYRYFTIQEPKEREISVADFRDRVVHHSLVNILEPIYEKRFIY
                     DSYATRKGKGTHRAISKAQQCMKANRWYLKMDVRKYFNSINHEILNGIIARTIKDPFI
                     LNLCAVIISKGGDGKTGLPIGNLTSQFFANVYLDIFDHFIKERLRVKNYVRYMDDFCV
                     FSDDKEYLKNIRNKATEFLDVTLRLEIKDKATLINNRLHGLPFLGVRIFTNMIRYKRK
                     NFNRSFKKIKTREWEYKNGFIDYEKYSSSMQSLTAHLTHYGNSLLKSRIYTGTVSKAG
                     LTA"
     gene            complement(2022..2360)
                     /gene="avd"
                     /locus_tag="P5123_13190"
     CDS             complement(2022..2360)
                     /gene="avd"
                     /locus_tag="P5123_13190"
                     /inference="COORDINATES: protein motif:HMM:NF033474.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diversity-generating retroelement protein Avd"
                     /protein_id="HRX17265.1"
                     /translation="MKDDYPLYVKWRYIVAYMLDISGKFPKNVRFNLADRLTNISLDV
                     LEAIIEAIYAKEKTDILRRANLYIEKIRALLQISVDKKYISIGQYEHISGEINEAGKM
                     IGGWVKSCVE"
     gene            complement(2357..>2796)
                     /locus_tag="P5123_13195"
     CDS             complement(2357..>2796)
                     /locus_tag="P5123_13195"
                     /inference="COORDINATES: protein motif:HMM:NF012779.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="HRDC domain-containing protein"
                     /protein_id="HRX17266.1"
                     /translation="FNSITKTFNTDDLNAFCVNKKVISTKIEFFQDEKHACWTVFLEY
                     EPILEKNGDVPSGLTEAGRLCYEKLREWRKETAEKEGIPPYVIAKNSQLGEIVKKEIK
                     TLEALKQLNGFGAKKIEKYGKDICGIVKAFYSDISEKLDEYIP"
BASE COUNT          729 a          596 c          522 g          949 t
ORIGIN      
        1 atcctgacct tcagctcgtt atcgcttata gaaatcttct cctcatcggg atctcctgtg
       61 aacggaaaga aatgcagttt taccctgtaa tcagtgtcgc tctgcagcct gtacatcttg
      121 ccaaggttaa ggctacgtgt aaaactttca ccgggcccaa gcttaatttc acgccttgtc
      181 atccacgaaa gagtttcttt tagatcactg cccgatgcca tatatggtac aatcagttcg
      241 gcatttcttc cggtcatgtc atagactacc ggtttgaatg tattataatc agcagtatct
      301 ccgcgctccc tgttaaacat atcttcacgt cccgggggat caaacacgta gaatgaagca
      361 acttcatctg ttatgttttt tattacaaca tgcagaatta tatcttcgct gttgtagaat
      421 ttcagcttct ccattttaag gtaaacgctg aatgaattga tgtcgccttt acgctgcctt
      481 actttgccgg gcactttttc ctggcttaaa gtcggcatcg acaataaaag cgctgtaata
      541 atcagggctg aatatatttt taatctgttc atatctctac cggtatatta ctatcttttt
      601 aatatatcga cataatcagg ttaataagtt atgaattatt ttttgttaca aggcattcaa
      661 agtacggttc ggactggaag tagttgtgcg aaagactttg cccccctctt ttgtttctgg
      721 ggaccggcca tccgtggccg aatttttcgt atcaaaaaaa taaaattctc accgcattcg
      781 gaagaaccta ccagaggtga gaaagttgta aatgtttgat cttaaaacaa tcagatgcgg
      841 atcacggtat ctggacttgc ggttatactg ttgaattatc catgaataat tcaatacaca
      901 caaacgcgcc tgtactcgca aagcggaagc ctatgttgtt gttctcgttg ttcggattgt
      961 tgttgttcca gttgccgacc tgcatgttgt cagcagaatt gttccaactg ccgccccgtt
     1021 tcacgcggtt agacccgctt ttgataccgt gcctgtatat attctgcttt tcagcagact
     1081 attaccataa tgtgttagat gagccgtaag gctctgcata ctgcttgaat acttttcata
     1141 atcaataaaa ccgtttttgt attcccactc tcttgttttt atttttttaa aactgcgatt
     1201 gaaatttttt ctcttatatc ggatcatatt cgtaaaaatg cgaacaccga gaaatggcag
     1261 accgtgaagt ctgttgttta tcaatgtggc tttatctttt atctcaagcc ttagcgtaac
     1321 gtctaaaaat tctgtcgctt tgtttctgat atttttcagg tattctttat catcagagaa
     1381 aacacagaaa tcatccatat atctgacgta gttctttact ctgagccgct ctttaatgaa
     1441 atggtcaaaa atatcaagat acacattggc gaaaaactgg ctcgtgaggt tgccgattgg
     1501 caggccggtt ttgccgtcac cgcctttaga aattatgaca gcgcatagat tcagaataaa
     1561 gggatctttg attgtccggg ctataatacc attaagaatt tcatgattga ttgaattaaa
     1621 atatttccgc acatccattt ttagatacca gcggttcgct ttcatacatt gctgtgcttt
     1681 tgatattgcc ctgtgggttc ctttgccttt tctggtagca taagaatcat agataaaccg
     1741 tttttcgtaa attggctcaa gtatgtttac cagcgagtga tgcacaaccc tgtctctgaa
     1801 atcagctaca gatatttcac gctctttcgg ttcttgtatg gtaaaataac gataatcacg
     1861 gggtatatac tcactggaag aaagttcctc tttcagtaga aaaagttcct tttcaagatt
     1921 gaaggtaaat tcacaggcag agtagttctt tgtggccttg aacgcctttt tatagccact
     1981 atagatgttt tcaaaagaaa gaaatgtttc tttcaggttg cctactctac gcatgattta
     2041 acccagcccc ctatcatctt acctgcttcg ttaatttcac cggatatatg ttcgtactgg
     2101 cctatcgata tatacttttt atcaacgctt atctgcaaaa gtgccctgat tttctcgata
     2161 tacaggttcg ccctgcgcaa tatatccgtc ttttcttttg catatatcgc ttcaattata
     2221 gcttctaata catcaagaga tatattggtg agtcggtcag cgaggttaaa acgtacgttc
     2281 tttggaaact tacccgatat atccagcata tacgcgacga tatatctcca ttttacatac
     2341 agcgggtagt catctttcat gggatatatt cgtcaagttt ctcggatata tccgaataga
     2401 atgctttaac gataccgcat atatccttgc cgtatttttc tatctttttc gcgccgaagc
     2461 cgtttaactg ctttaacgcc tcaagggttt ttatctcttt ctttacaatt tcaccaagtt
     2521 ggctgttttt cgctatgaca tagggaggaa tgccctcttt ttccgctgtt tctttgcgcc
     2581 attcacggag cttttcgtag cagagcctgc ctgcctctgt gaggccagaa ggcacatccc
     2641 cgtttttctc taaaatcggt tcgtattcga ggaatactgt ccagcaggca tgcttttcgt
     2701 cttgaaaaaa ctcaattttt gtggaaatca cctttttatt aacgcaaaaa gcgttcaaat
     2761 catccgtatt gaaggttttt gtaatagaat taaagg
//
LOCUS       DAOTGZ010000302         2783 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_3064
            NODE_42928_length_2783_cov_4.423387, whole genome shotgun sequence.
ACCESSION   DAOTGZ010000302 DAOTGZ010000000
VERSION     DAOTGZ010000302.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868088
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 2783)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2783)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:22:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,785
            CDSs (total)                      :: 2,751
            Genes (coding)                    :: 2,732
            CDSs (with protein)               :: 2,732
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 12 of 19
            Pseudo Genes (internal stop)      :: 5 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2783
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_42928_length_2783_cov_4.423387"
                     /isolate="AMR_MDS_3064"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..357
                     /locus_tag="P5123_13200"
     CDS             <1..357
                     /locus_tag="P5123_13200"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRX17267.1"
                     /translation="MAEQRERWNDYLKYIDLEQYIYSFPDYAKAYLSGGDVVKREALR
                     DFSTYLIKSRPDTISEYKIVSETVEDGRAKVSVLIKRAAAGMPFTYNYTYSLEKKSAV
                     WMITGVTASVARDIRQ"
     gene            354..1379
                     /gene="fliM"
                     /locus_tag="P5123_13205"
     CDS             354..1379
                     /gene="fliM"
                     /locus_tag="P5123_13205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020768525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009288 - bacterial-type flagellum
                     [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar motor switch protein FliM"
                     /protein_id="HRX17268.1"
                     /translation="MTEILSQDEIDSLLTAISTGEHEMEDVAPTEQRKVKLYDFRRPD
                     KFSKDQIRTLQMMHETFARLTTTALSAQLRALVSVHVASVDQLTYEEFVRSIPNPTTL
                     AVVNMDPLKGSAVLEIDPSITFTIIDKLFGGEGEATKISRELTDIELSVMEGIVVRIL
                     GNLREAWSNVIDLRPRLGNIETNPQFAQIVPPNEMVVLITLETKVGEVEGMTNLCLPY
                     ITIEPVISKLSAQYWFSSIRKGATDENLQIIQNRLENVTLNVVAEIGDVEVSMQEVLN
                     LSLGDVIKLSKTKTDSDMTLKIEGRRKFKCRPGLVGNRVSVQVGERIEEVPDELLITK
                     KEEDEMM"
     gene            complement(1474..1923)
                     /locus_tag="P5123_13210"
     CDS             complement(1474..1923)
                     /locus_tag="P5123_13210"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRX17269.1"
                     /translation="MEVSDFRTSIDVEAGRLEKIEKYAEKYNMDISDVINTVLDKYSK
                     ILVKNPVCNNTLTKYLERGTNYKSVHIRFSIIAKRKLKDIANLYCYSLSYMLFLAMDF
                     FEEFMDGVKEFIRPCFHASFVHHFFGFPYFINSWGMNFYEGKTHPSG"
     gene            2227..>2783
                     /gene="xseA"
                     /locus_tag="P5123_13215"
     CDS             2227..>2783
                     /gene="xseA"
                     /locus_tag="P5123_13215"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: protein motif:HMM:TIGR00237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009318 - exodeoxyribonuclease VII
                     complex [Evidence IEA];
                     GO_function: GO:0008855 - exodeoxyribonuclease VII
                     activity [Evidence IEA];
                     GO_process: GO:0006308 - DNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII large subunit"
                     /protein_id="HRX17270.1"
                     /translation="MNNTPLSITELSSVIKQTIEGNSLLSNVAVIGEVSNVTNHSSGH
                     IYFTLKDSGAVISAAFFKYANRSCKVRLKEGLKVIALGSVTVYEKRGSYQLIVKSLIE
                     DGIGELQRQIQELKERLLKEGLFDDERKKSLPFLPRRIGIVTSPTGAAFRDILKVLLR
                     RFPNVEVVLAPAKVQGSDASVSIVRA"
BASE COUNT          828 a          521 c          604 g          830 t
ORIGIN      
        1 atggccgagc agcgtgaaag atggaatgat tatttaaaat acattgatct tgaacagtac
       61 atttactctt ttccggatta tgccaaagca tatctttcgg gtggagacgt tgtaaaaaga
      121 gaggctttga gagacttttc tacatattta atcaagagca ggcctgacac cataagtgag
      181 tataaaatag tttctgaaac ggtagaagac ggtcgtgcca aggtttcggt attgataaaa
      241 agagctgcag ctggaatgcc ttttacatat aattatactt attcgcttga aaaaaagagt
      301 gctgtttgga tgattaccgg tgtaacagct tcggttgcga gggatatacg acaatgacag
      361 aaatattatc acaagacgaa atagattccc tattaacggc gatatcgacc ggtgaacacg
      421 aaatggaaga tgttgcgcct accgaacagc gcaaggtcaa actgtacgat ttccgtcgcc
      481 ccgacaagtt ttctaaagac cagattagaa ctttgcagat gatgcacgag acctttgctc
      541 gtcttacaac aacagctctg tcagcccaat tgcgagctct ggtaagtgta cacgttgcat
      601 cggttgatca gcttacgtat gaagaatttg taagatcgat acctaatccg actacacttg
      661 ccgttgtaaa tatggatccg cttaaaggat cggctgtatt agagatagac ccttcaatta
      721 cctttaccat tattgataaa ctgttcggtg gtgagggcga agccactaag atttcgcgtg
      781 aattaacaga cattgagctt tcggtaatgg aaggtatcgt tgttcgtata cttggaaatc
      841 ttcgtgaagc gtggtcaaac gtaattgatc ttcgtcctcg cttaggaaat atcgagacta
      901 atccgcagtt tgcccagatt gtcccaccta atgagatggt ggtacttatc accctcgaga
      961 caaaggttgg tgaagtagaa ggtatgacta acctatgtct tccttatatt acaatagagc
     1021 ctgttatttc aaaactttcg gctcagtact ggttttcgag tattcgtaaa ggtgcaactg
     1081 acgagaactt gcagataatt caaaatcgtc ttgaaaacgt aaccttaaat gttgttgcag
     1141 agattggtga tgttgaagta tccatgcagg aggtattgaa cctttcactt ggtgatgtta
     1201 ttaaactttc gaaaacaaaa accgattcag atatgacatt gaagattgag ggcagacgca
     1261 agtttaaatg ccggcctggt ctggttggaa atcgtgtatc tgttcaggtt ggcgaacgta
     1321 tagaggaagt tcctgatgaa ctgcttatca ccaaaaaaga agaagatgag atgatgtgat
     1381 tttttagttc ttttctgttt tttctgttct ttgttcctgt tttgaataaa aaaatcgaaa
     1441 caaaacccag aaattcgtcg ttttccgggt tttttagcca gatggatggg ttttgccctc
     1501 gtaaaagttc ataccccagc tattaataaa gtatggaaac ccaaagaagt gatgaacaaa
     1561 agaggcatga aaacagggac gaatgaactc cttaacacca tccataaact cctcgaagaa
     1621 atccattgca agaaacaaca tgtacgacaa gctgtaacag taaaggttgg ctatatcttt
     1681 aagctttctt tttgcgataa ttgagaagcg tatatgtact gatttataat tagtacctct
     1741 ctccagatac ttggtaagag tattattgca tacaggattt ttaaccagta tctttgaata
     1801 cttgtccagt acagtattta ttacatccga gatatccata ttatacttct cggcatactt
     1861 ctcgattttt tccagtctgc ctgcttccac atcgattgat gttctaaagt ctgaaacttc
     1921 cattaagtgc ctccagggat ttgtctgtat atatataaac gcagttagtg gcttttttgt
     1981 taaaattttt ttgtaagata ttcaatttgt agaaaaatcg acggatttgt ttcttttccc
     2041 tattgtgttt ttcgtaacgg caaacttgct taaagttaaa tatcagaggt tataacaaga
     2101 tatagctgat ctgatataag gacttgacgg gatttccacc cgattataga gtagaaagac
     2161 cttttcgaaa tccggtagag ggcaggcctg aaaggctgaa aacgcactat gccataacgg
     2221 gatcagatga acaatacgcc actatcaata acagagcttt cctccgtgat aaagcagaca
     2281 attgagggta attccctgct ttcaaatgtt gccgtgatag gagaggtttc gaatgttact
     2341 aatcactcgt ccggccatat atattttact cttaaagatt caggggcggt aataagcgcc
     2401 gcgtttttca aatacgcaaa tcgttcgtgc aaggtgcgtc tgaaagaggg gctcaaggtt
     2461 atagctttgg gttcggtaac cgtttacgaa aaacgtggaa gctatcagct tattgttaaa
     2521 tcgcttattg aagacggaat cggcgagctg caacggcaaa tacaggagct aaaagagaga
     2581 cttttaaaag aagggctctt tgatgacgag cgtaaaaaat cacttccttt cctcccccgg
     2641 cgaataggca ttgtaacatc gccaaccgga gctgctttcc gtgatatttt aaaagttctt
     2701 ttgcgccggt ttcccaatgt agaagttgtt ctggcacccg cgaaagtaca gggttctgat
     2761 gcctctgtat ccattgtccg tgc
//
LOCUS       DAOTGZ010000303         2756 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_3064
            NODE_43490_length_2756_cov_3.624583, whole genome shotgun sequence.
ACCESSION   DAOTGZ010000303 DAOTGZ010000000
VERSION     DAOTGZ010000303.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868088
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 2756)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2756)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:22:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,785
            CDSs (total)                      :: 2,751
            Genes (coding)                    :: 2,732
            CDSs (with protein)               :: 2,732
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 12 of 19
            Pseudo Genes (internal stop)      :: 5 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2756
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_43490_length_2756_cov_3.624583"
                     /isolate="AMR_MDS_3064"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..180)
                     /locus_tag="P5123_13220"
     CDS             complement(<1..180)
                     /locus_tag="P5123_13220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008759768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil-DNA glycosylase"
                     /protein_id="HRX17271.1"
                     /translation="MDVRIEDSWKTRLETEFSRDYFIRLREFVKHEYSVSTVFPPGRL
                     IFRAFDLCPFDKVNVV"
     gene            408..1289
                     /locus_tag="P5123_13225"
     CDS             408..1289
                     /locus_tag="P5123_13225"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF025054.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HRX17272.1"
                     /translation="MDRVLCVGAYDISQAIILLRIRVIKQSNLHYFKDIIMKKADECN
                     NIQDVRDSIDKIDRSLIQLISARSEFVQKAATFKKSEADVRAKDRVASMLIKRRQWAT
                     ENGVAPDFIETLFKMMVSHFIGRELKEWEQDSSPENPILIKKAKETDLPDILFMQKRA
                     FIQEAELNGCDYNIPPITQDIQSITKEYSESLFLKAVLDGQIAGSVRAKMENSVCKIV
                     RLVVEPLYQGKGIGKKLLSAIEYEFAEAKEFELFTSINSTRNRKFYNNAGYVEESEFD
                     IPNSPRMIGMKKTNCQI"
     gene            complement(1333..1797)
                     /locus_tag="P5123_13230"
     CDS             complement(1333..1797)
                     /locus_tag="P5123_13230"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF014566.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0071586 - CAAX-box protein processing
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane metalloprotease"
                     /protein_id="HRX17273.1"
                     /translation="MLLPSIAIFPVYWLAMVFLGGGQEEIGWRGYIMPFLEKRFGVFY
                     GTVILGLVWAIWHLPLWFIPGSNQTYMNFCVFVMLTTGYSFIFSWGLKKAGNRPMSGL
                     VMHGTANAFVPVFPVMVLNHNVSQPRYWIWVSLMLICGITLYLFSNHNNDSE"
     gene            complement(1834..2148)
                     /locus_tag="P5123_13235"
     CDS             complement(1834..2148)
                     /locus_tag="P5123_13235"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRX17274.1"
                     /translation="MNKNEKFPLCYFFLTFIWSWSLWVAVALSLGQTSNLTIESASPL
                     VIIVMVIGAFGPAFGAILSILTIEGKGAIIPFIKRFVDLRFGITAWIVPFLVLSVGTF
                     MA"
BASE COUNT         1002 a          507 c          487 g          760 t
ORIGIN      
        1 aaccacattg accttgtcaa aaggacaaag atcaaaagcc cgaaagatca accgtccagg
       61 cggaaacacc gttgacacag aatattcatg tttaacaaat tcccttaacc tgataaaata
      121 atcccttgaa aactccgttt caagtcgggt tttccaagaa tcctcaattc ttacatccat
      181 ctattagcta cctgtcattt tactgtgaat tttaaaaaat ggcacaaatt gaaagactgt
      241 actataaagt caattatcca aagccttata tatttgaagt gaaccataac agcttacaac
      301 cattttttaa aaattgttag gggtttgtca cttatacaaa aaaatcatat agcctttcta
      361 aaaataataa tttttggcaa atttctcatt aagagagttg ccaaaaaatg gatcgtgtgt
      421 tatgcgtagg agcgtatgat atttcacagg ctattattct acttcgaatt agagtgatta
      481 aacagtcaaa tttgcattat ttcaaggata ttattatgaa aaaagcggat gagtgcaaca
      541 acattcaaga tgtaagagac tctatagata aaattgacag atctcttatt caattgattt
      601 cagccagatc agaatttgta caaaaagcag ccactttcaa gaaatccgaa gccgatgtaa
      661 gagccaaaga tcgtgtagct tctatgctga taaagcgccg tcaatgggcc acagaaaacg
      721 gcgtggctcc agattttatc gaaactcttt tcaaaatgat ggtgagccat tttattggac
      781 gggaactcaa agagtgggaa caggatagct cgccggaaaa tccgatatta attaaaaaag
      841 caaaagaaac agatttacct gatattttat ttatgcaaaa aagagccttt attcaggaag
      901 ccgaattaaa tggttgtgat tataatatcc ctccaattac acaagacatc cagagcataa
      961 caaaagagta ttctgagagc ctattcttaa aagctgtttt ggatggtcag atagctggat
     1021 ctgttagagc aaaaatggaa aacagtgtgt gtaaaatagt acgcctggtt gttgagcctt
     1081 tgtatcaagg gaaaggaata ggaaaaaaat tactttccgc aattgaatac gagtttgccg
     1141 aagcaaaaga gtttgaactt tttacttcta ttaatagtac aagaaacaga aagttttaca
     1201 ataacgcagg ttacgtcgaa gagtctgagt ttgatatacc gaactctccg cgcatgatag
     1261 gcatgaaaaa aactaactgc cagatttgac agttagtttc aaaatatcgg attgaatctt
     1321 ttcacacccg attcattctg aatcattatt atggtttgaa aacagataaa gagtaatacc
     1381 acagataagc atcaaagaaa cccatatcca gtatctcggc tgtgagacat tgtgattcaa
     1441 aaccattaca gggaaaaccg gaacaaaggc attcgcggtg ccatgcataa caagaccaga
     1501 cattggtcta ttgccagctt ttttcaagcc ccatgaaaaa ataaaggagt aaccggttgt
     1561 gagcattacg aaaacacaaa aattcatata tgtctgattt gatcctggaa taaaccagag
     1621 cggaaggtgc catatagccc aaacaagacc taaaataact gttccataaa aaacaccgaa
     1681 acgtttttca agaaaaggca ttatgtaccc cctccaaccg atctcctctt gacctccgcc
     1741 caaaaaaacc atcgctaacc aatagacagg gaaaatcgca atcgatggta gcaacatctt
     1801 tagtctgggt aagccgaaaa gttcgggaat tatctaggcc atgaaagttc ccacagaaag
     1861 aacaagaaat ggaacaatcc atgcagtaat cccaaaacgc agatcaacaa aacgtttaat
     1921 aaaaggaatt atcgcccctt tcccctctat tgttaaaatt gatagaatag caccaaaggc
     1981 aggtccgaaa gctccaataa ccatgactat tataaccaga ggtgacgccg attcaatagt
     2041 tagattcgaa gtttgcccta acgataaagc aaccgcaacc cacaatgacc agctccaaat
     2101 aaaggttaag aaaaaatagc aaagagggaa tttttcgttt ttattcattt tggcatcctt
     2161 atttatatta aaattaaact ttttaaacta attttgaaga ttcattgttg aaatttatat
     2221 caattcaaaa taaacaatca agtaattcaa aagtgctacc ttttatattc acagcaatta
     2281 tctaataaat gattatcgaa aagctataat gattgaatga aatcagatta taatattaac
     2341 agatgattgt gcttacgcga gctttatcgg cagacacttg tttcagtttt ctttggttat
     2401 cttttccact tagcgtctct ggcactccgc aaaaaaggca atgaacaatg aataacgaaa
     2461 aattagaaag aacagtattt accaagggag ctcagctgac caatatttac tggaaataat
     2521 ccgtatattg tcgttcctgc ggatgcagga atttatagag taaattagtc atcgcctggg
     2581 attccggcac tccgaaggaa ggtaatgaat agtaaataat gaaaaacaga tctttatctg
     2641 catacaagaa tatagcgagg agagtatcgt aaagacgcac cggtgaatta tttaatagaa
     2701 actatctctt tttttgtaag gccagttaac tctgctattt cttgtacgtt aaaacc
//
LOCUS       DAOTGZ010000304         2715 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_3064
            NODE_44374_length_2715_cov_5.050752, whole genome shotgun sequence.
ACCESSION   DAOTGZ010000304 DAOTGZ010000000
VERSION     DAOTGZ010000304.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868088
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 2715)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2715)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:22:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,785
            CDSs (total)                      :: 2,751
            Genes (coding)                    :: 2,732
            CDSs (with protein)               :: 2,732
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 12 of 19
            Pseudo Genes (internal stop)      :: 5 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2715
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_44374_length_2715_cov_5.050752"
                     /isolate="AMR_MDS_3064"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            70..930
                     /gene="ispE"
                     /locus_tag="P5123_13240"
     CDS             70..930
                     /gene="ispE"
                     /locus_tag="P5123_13240"
                     /EC_number="2.7.1.148"
                     /inference="COORDINATES: protein motif:HMM:TIGR00154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050515 - 4-(cytidine
                     5'-diphospho)-2-C-methyl-D-erythritol kinase activity
                     [Evidence IEA];
                     GO_process: GO:0016114 - terpenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-(cytidine
                     5'-diphospho)-2-C-methyl-D-erythritol kinase"
                     /protein_id="HRX17275.1"
                     /translation="MKEKAYAKINLHLAVKEKRADGYHNIESVMFSVSLCDEITVLVC
                     EKKKTGGSQLEIEAYGRFGSVIQDLPLEKNLVYKAFILFFENVSYNVYMKIRLNKRIP
                     AGAGLAGGSSDAAAMLRLLNRIFHCYSFKKLMMMGSKIGADVPFCLQKKPAFCIGIGN
                     IISVYNKLRLKIWVVITFNDCHISTKEAYQNLLSCNSNTEDCKKIKELIYLSEFRYYF
                     NSFENYAIKLFSEIANIKKFMYDYGAFFSLMTGSGSSVVGYFKSKQTACKAAGFMNEK
                     GFDSVVSYLI"
     gene            933..1004
                     /locus_tag="P5123_13245"
     tRNA            933..1004
                     /locus_tag="P5123_13245"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:965..967,aa:Gln,seq:ttg)
     gene            1019..1741
                     /locus_tag="P5123_13250"
     CDS             1019..1741
                     /locus_tag="P5123_13250"
                     /inference="COORDINATES: protein motif:HMM:NF012695.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NTP transferase domain-containing protein"
                     /protein_id="HRX17276.1"
                     /translation="MRAIILAAGKGVRMNSSLPKVLHRINNVPLVQHVINSLNKINVE
                     SICVIVGYKADEVQKEIGDSVLYAYQKEQLGTGHAVLQAESCFKNYNGPVIITCGDAP
                     FITPQSFKDMLSLCDDEKCKASVLTMICENPNGYGRIIKDKNGDVAEIVEEKDATDSI
                     KQIKEVNCGTYVVDSCLLFEGLKSVGTDNAQNEYYLPDIVKYIISKGYLVKSAVLSDN
                     REGIGINSKEELKQAEKIYNEF"
     gene            1731..2690
                     /locus_tag="P5123_13255"
     CDS             1731..2690
                     /locus_tag="P5123_13255"
                     /EC_number="2.7.6.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013119053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004749 - ribose phosphate diphosphokinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0006015 - 5-phosphoribose 1-diphosphate
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0009156 - ribonucleoside monophosphate
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0009165 - nucleotide biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-phosphate pyrophosphokinase"
                     /protein_id="HRX17277.1"
                     /translation="MNFDITKKKYDIKIISCNSNLTLANDITDKLGVSLTESDCKKFL
                     DGEIFVKIKESVRGCDVFIVQSTCKPANDNLMELLLMIDAAKRASARTITAVIPYFGY
                     SRQDRKMEPRVPISAKLVANLLMKAGIQRVLTMDLHADQIQGFFDVPVDNLFLTPIAI
                     GYFNSLKIEDLVVVSPDSGGTDRARYLAKHLQCGLAIIDKRRPKANVCEIMNVIGEVE
                     GKNCLLIDDIIDTAGSISGGAKALKQFGAKDIYCIATHPVLSGNAVSNLKGAGFKEII
                     FSDTIPIPEDKMLDNMTILRTCSLFSEAIKRIYSGESVSNLFV"
BASE COUNT          876 a          375 c          588 g          876 t
ORIGIN      
        1 aattaattgt cttgacctgc gaaagggtgc ataatatttt tgctctgaaa ttgactgaga
       61 agtagtgatt tgaaagaaaa agcttatgct aaaattaatt tgcatttggc tgtaaaagag
      121 aaacgagctg atggttatca taacatagag tcagtcatgt tttcggtttc tttgtgtgat
      181 gagataacgg ttttggtttg cgaaaaaaag aaaaccggag gctctcaatt agaaattgag
      241 gcttatggtc ggtttggttc ggtaatacaa gatttacctt tggaaaaaaa tcttgtttat
      301 aaagctttta ttttgttctt tgaaaacgta tcttataatg tttatatgaa gattcggctt
      361 aacaaaagga tcccggcagg tgccggactt gcaggaggca gtagtgatgc ggctgcaatg
      421 ttaagactgt tgaatagaat ttttcattgt tatagtttca agaagttaat gatgatggga
      481 tccaaaattg gagcagatgt tcctttttgt ttgcaaaaaa agccggcttt ctgtataggc
      541 attgggaata taatatcggt atataataaa ctccggttaa aaatatgggt tgtaatcact
      601 tttaatgatt gtcatattag taccaaagaa gcttatcaaa atttattaag ctgcaattcc
      661 aatacagagg attgtaaaaa aattaaagag ttgatatatt tatcagaatt tagatattat
      721 tttaatagtt tcgagaatta tgctattaag ctgttttcag aaatagcaaa tataaagaag
      781 ttcatgtatg actatggagc atttttttct ttaatgactg ggtccggttc gtctgtggtt
      841 gggtatttta aatcaaagca gacggcttgc aaagcggcag gttttatgaa cgagaaagga
      901 tttgattctg tagtatcata tttgatataa attgaggcgt agccaagcgg taaggcaccg
      961 ggttttggtc ccggcattcc aaggttcgaa tccttgcgtc tcagagagaa ggtaaagtat
     1021 gagagcgatt attctggcgg caggtaaagg agtaagaatg aattcttctt taccaaaggt
     1081 tttgcatcgt attaataatg tccctcttgt acagcacgta attaactctt tgaacaaaat
     1141 aaatgttgaa tctatctgtg taattgtggg ttataaggca gatgaggttc aaaaagagat
     1201 tggtgattca gttttatatg cttatcagaa agaacagttg ggaaccggtc atgcggtgtt
     1261 gcaagctgaa tcctgtttta aaaactacaa tgggccggta attattactt gtggagatgc
     1321 tccatttata acacctcaaa gctttaaaga tatgctgtct ctctgtgatg atgagaaatg
     1381 taaagcttct gttttaacaa tgatatgtga gaatccgaat gggtatggcc ggattattaa
     1441 agataagaat ggcgatgttg ctgaaattgt cgaagaaaag gatgctactg attcaataaa
     1501 acagataaaa gaagtaaatt gcggtacata tgtagttgat tcctgcctgc tgtttgaagg
     1561 attaaaatct gttggaacag ataatgccca aaatgagtac tatctaccgg atatcgttaa
     1621 atatatcatt tccaaagggt atcttgttaa gtccgcagtt ctttcggata atcgtgaagg
     1681 tattggaata aatagtaaag aagaactgaa acaggcagag aaaatatata atgaattttg
     1741 atattactaa aaagaaatac gatataaaaa ttataagctg taattctaat ttaacactgg
     1801 ctaacgatat aacggataaa ctgggtgttt ctttaactga gtcagattgt aaaaaatttc
     1861 tcgatggaga aatttttgta aagataaaag agagtgtccg tggatgcgat gtttttattg
     1921 ttcagtccac ttgtaaacct gccaatgata atctaatgga actccttctt atgatagatg
     1981 ctgcaaagcg cgcttcggct cgtactataa cagcagttat tccctatttc ggttattcga
     2041 gacaagatag aaaaatggag cctcgcgttc caatttcagc caaacttgtt gcgaacttat
     2101 taatgaaagc tggaattcag cgtgtattaa caatggattt acatgcggat caaattcagg
     2161 gattttttga tgttccggta gacaatctct ttttaacacc gatagcgatt ggatatttca
     2221 attcattaaa aattgaagac ttagtggttg tttctcctga tagtggaggt actgaccggg
     2281 cgcgctatct tgcaaagcat ttacaatgcg gtttagctat tatcgataaa cgtcgtccaa
     2341 aagctaatgt ttgcgaaatc atgaatgtga ttggagaggt tgaggggaaa aattgtcttt
     2401 taatagatga tattattgat actgcaggtt caatttctgg cggagccaaa gccttaaagc
     2461 aatttggagc caaggatata tattgtattg ccacccatcc cgtgctgtct ggtaatgcgg
     2521 tctcaaatct aaaaggtgcc ggttttaagg aaataatatt ttcagatacg attccgatac
     2581 ctgaagataa aatgttggat aatatgacaa ttcttcgaac atgttctctt ttttcagaag
     2641 cgatcaagag aatatattca ggagaatctg taagtaatct tttcgtataa taatatttta
     2701 gtgaggaaaa aatgg
//
LOCUS       DAOTGZ010000305         2703 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_3064
            NODE_44631_length_2703_cov_4.307402, whole genome shotgun sequence.
ACCESSION   DAOTGZ010000305 DAOTGZ010000000
VERSION     DAOTGZ010000305.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868088
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 2703)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2703)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:22:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,785
            CDSs (total)                      :: 2,751
            Genes (coding)                    :: 2,732
            CDSs (with protein)               :: 2,732
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 12 of 19
            Pseudo Genes (internal stop)      :: 5 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2703
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_44631_length_2703_cov_4.307402"
                     /isolate="AMR_MDS_3064"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(175..1845)
                     /locus_tag="P5123_13260"
     CDS             complement(175..1845)
                     /locus_tag="P5123_13260"
                     /inference="COORDINATES: protein motif:HMM:NF012862.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="caspase family protein"
                     /protein_id="HRX17278.1"
                     /translation="MKRLLFFSIIFLSVLPASLIASSVKRYAFIVGANNGGNDRVLLK
                     YAVSDAATFRSVLNSIGGVADEDVFVLYNPDPRNFYSSLGRMTKKINSDKKKYRKMEL
                     FFYYSGHSDQNGILLGNDKITYAELKKNIKSLDVDVRIVILDSCSSGAFTRTKGGTMK
                     PAFFVDNSYDMKGNAIMTSSSADEVSQESDAIRGSFFTHYVLTGMRGAADVNQDKKIT
                     LNEVYQFAYNSTLSRTQKTTGGPQHPNYHIEMIGTGDVVLTDINRASSKIVMHRDLEG
                     RFFLRDSSNNVVAEFQKSYGEEFSIAVGSGKYVVVNNHYNNSISEASINVKEGSAYNL
                     KTSDFSGGSKEKTRWRGEKEEVFADKVKYKMEKLFGEDGDSGDYLLRSGNVRFSGMGG
                     PMYYMLPFAESHASFMGGAGGVIINDSWVFGAGGFGLVNPTKASDYSDSFQELEEQYL
                     VLGYGGFFFNYYFNPKSIVTYSVGFMLGGGSCTVVSDLDQDPEDYNNSSDFFAFYPFL
                     NVNVNMTRFFRVGAGVGYNYFSKLNVDYIKEDEFNNITINVFAQFGWF"
     gene            complement(1849..2661)
                     /locus_tag="P5123_13265"
     CDS             complement(1849..2661)
                     /locus_tag="P5123_13265"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRX17279.1"
                     /translation="MNNDFITDLTLEQYLLHELPENEYEQVADAIEKDSALRLRLLKI
                     EESNHDFFTAHPVSLNVSLITKAVEKSKSNSDKRIPKAPSFLSQIFSLKNAFALSAAL
                     SVIVALFVFMPRNNKVLTGLPDQMNDVTRIKGSTGIMIYSKGSAGVTLLQNRSSAKPG
                     DHLQIVYQNDNNTFGAILSIDGNGTITKHLPQDGEVSRSIERGSRVVLQNSYVLDNAP
                     HFEKFYFFVSKKPFQIQLLRDRLKEAGNGNPESDPYLDDYQIQYKSFILYKE"
BASE COUNT          881 a          592 c          429 g          801 t
ORIGIN      
        1 aatttcaata gaccaaacaa taaaacaaca aaaaccccaa aaaccccaaa aaccccaaaa
       61 acataaccaa tatttatcgg aaggggttta tagtcaataa atatatttta tcttaaaccg
      121 atttcttctt aatgataacc caaaataatc gaagtactta aagcagatca ggaactaaaa
      181 ccaaccaaac tgtgcaaaaa cattaatcgt aatattatta aactcatctt ccttgatata
      241 atcaacattt agtttagaaa aatagttata acctactccg gctccaacgc gaaaaaacct
      301 agtcatattc acattaacat ttaaaaacgg ataaaaggca aaaaaatcgg aactattatt
      361 ataatcttcg ggatcttgat ccagatcact gacgacagta caacttcctc cccccaacat
      421 aaaacctacc gaataggtta ctatactttt tggattaaaa taatagttaa agaaaaagcc
      481 accgtaccca agcactaaat actgctcctc tagctcttga aaactatcag agtaatcact
      541 tgccttagta ggattcacaa gaccaaaacc cccggcacca aatacccaac tgtcattaat
      601 gataacccca ccggctcccc ccataaagct ggcatgagat tctgcgaaag gaagcatata
      661 gtacatggga ccacccattc ccgaaaacct gacatttcca cttctcagga gataatctcc
      721 ggaatctccg tcttctccga aaagcttttc cattttatat tttaccttat cagcgaagac
      781 ttcttccttc tctccacgcc atcttgtctt ctcttttgaa cctccggaaa aatccgaagt
      841 tttaagatta taggcacttc cctctttgac attaatagat gcttctgaaa tagaattgtt
      901 atagtgatta tttaccacca catattttcc ggatccaact gctatcgaaa attcctcacc
      961 ataagatttt tgaaattcgg caacaacatt attggaactg tctcttagaa agaatcttcc
     1021 ctctaaatcc cgatgcatta caattttaga ggaagcgcga tttatatcgg ttaatacaac
     1081 atcaccggtt ccaatcatct caatatgata attagggtgt tgcggacctc cagtagtttt
     1141 ttgagtcctt gataaggtcg aattataggc aaactgatag acctcgttaa gagtaatctt
     1201 tttatcctga tttacatccg ccgcgccgcg cataccggtc aaaacataat gagtaaaaaa
     1261 ggacccgcgt atagcatccg attcctgaga aacttcatcg gcagaacttg acgtcataat
     1321 agcgtttcct ttcatgtcgt atgaattatc gacaaaaaag gccggtttca ttgtcccgcc
     1381 ttttgttctg gtaaaggctc cggaagaaca tgagtcaaga atcacaatgc gcacatctac
     1441 atccagagac ttgatgtttt tcttaagttc cgcataggta attttatcat ttcccagcaa
     1501 aattccgttc tgatcagagt gccctgaata gtaaaaaaag agctccattt tacggtattt
     1561 ttttttgtca ctgtttatct tttttgtcat tcgtcctaat gacgaataaa aattccttgg
     1621 gtcagggtta taaaggacaa agacatcttc atcagctaca ccaccaatcg agttcaaaac
     1681 tgatcggaaa gtcgctgcat cagaaaccgc gtatttaagc agtacccggt catttccgcc
     1741 attatttgct ccaactataa atgcatatcg tttaactgat gaagcaatta ggctggcagg
     1801 cagtacagat aaaaaaataa tcgaaaagaa taaaagcctt ttcatctttt actccttata
     1861 caagataaaa gatttatact gtatttggta atcatcgaga tacggatcag attcagggtt
     1921 tccgttacct gcttctttta aacgatctct caaaagctgt atttgaaacg gtttttttga
     1981 cacaaagaaa taaaatttct caaaatgagg cgcattatca agaacataag aattttgcag
     2041 cactacccgg cttccccgct caatcgagcg tgatacttcg ccatcctgag gcaaatgttt
     2101 tgttatagtt ccattaccgt cgatagaaag aattgcccca aaagtattat tgtcattttg
     2161 ataaactatc tgaagatggt cgcccggctt cgctgaactt ctattttgaa gtaaggtcac
     2221 tcccgcacta cccttactat aaatcattat accggtgctc ccctttatcc gcgtaacatc
     2281 attcatttgg tcaggcaatc cggtcaacac cttattattc ctgggcataa agacaaaaag
     2341 agcaacaatt acagaaagag ccgctgataa agcaaatgcg ttttttaaag aaaaaatctg
     2401 cgacagaaat gatggtgctt ttggtattct cttatctgaa tttgactttg atttttcaac
     2461 agcctttgtt atcagtgata catttagcga gacagggtga gccgtaaaaa aatcatgatt
     2521 tgactcttct atttttagaa gcctaagacg aagtgcagag tccttttcaa tagcatctgc
     2581 tacttgctcg tactcatttt caggaagttc atgaagaaga tattgttcaa gtgttaaatc
     2641 agtaataaaa tcattgttca tgataatcct ccctgaaaga tgcagcaatc tgttttacct
     2701 tgg
//
LOCUS       DAOTGZ010000306         2690 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_3064
            NODE_44898_length_2690_cov_6.697913, whole genome shotgun sequence.
ACCESSION   DAOTGZ010000306 DAOTGZ010000000
VERSION     DAOTGZ010000306.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868088
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 2690)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2690)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:22:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,785
            CDSs (total)                      :: 2,751
            Genes (coding)                    :: 2,732
            CDSs (with protein)               :: 2,732
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 12 of 19
            Pseudo Genes (internal stop)      :: 5 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2690
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_44898_length_2690_cov_6.697913"
                     /isolate="AMR_MDS_3064"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            449..1210
                     /locus_tag="P5123_13270"
     CDS             449..1210
                     /locus_tag="P5123_13270"
                     /inference="COORDINATES: protein motif:HMM:NF016501.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar filament outer layer protein FlaA"
                     /protein_id="HRX17280.1"
                     /translation="MKREVKTLLVTILAGFLFVLAGDFNIGLIAQDAAGDDAAPAARG
                     GERKKLDGLVVDWMNDFEDSEDWRAVSTCPLGDTKIRKMPGKPNKVNEDGTVEDAKIE
                     LTDENGVTHKDEYVLGAKTYFADRGFDRVEVFPPNEYIVRGKAKEVRVWVLGRKFRHT
                     LYIKLRDYRGKIHKIKVGRLDFWGWKEMSVIVPGWLPQSAKYAMMDKNLHFVSFFVES
                     DKFEVPGTFYFYMDNFRIVTDLSEFTGDPDIKDNW"
     gene            1282..2085
                     /locus_tag="P5123_13275"
     CDS             1282..2085
                     /locus_tag="P5123_13275"
                     /inference="COORDINATES: protein motif:HMM:NF016501.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar filament outer layer protein FlaA"
                     /protein_id="HRX17281.1"
                     /translation="MKRRYFKIIMFILSLSIFGLLVTTVYSQLITNVPPDISIDEIKA
                     LVVEDFESSPTWVVETEPKPYTKGGGDEKAKKNPVPVLEIKIVDGAPSDMKPQEWQAD
                     NKGTKKDKVLGVNFRFKYPGHNTVKMTPPEPINLPGRARGLSIWVHGRGNKYYLECWV
                     KDYEGSTHILKFGEVGFVGWRPMSVEIPEFIPQAVDSYPSTKTLTLEKFVLRSDPRER
                     VGNVFFFFDQLKVLTQTYEVNFDGSGLEDSFESSKGTRETKPAPAGGTE"
     gene            2183..>2690
                     /locus_tag="P5123_13280"
     CDS             2183..>2690
                     /locus_tag="P5123_13280"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRX17282.1"
                     /translation="MQQFIDRLESLLKEESFGRMGASSVGISRFKNLESFFNECVQGN
                     ATKEIYDACDNHLDENPSSVTSLYISGLIAFHCNIIDGKNNLISLIKTFSDLQKWAVV
                     EHLADKVLEYGENSTALKALAMSLEKLKRRKEAVPVWEELIKINRFDADIARKLSQTL
                     EEEDQAKSV"
BASE COUNT          802 a          416 c          611 g          861 t
ORIGIN      
        1 ctaaagatgc ttaataaata accattttct ttagtgctag cagagaggcc aagtgtctct
       61 ctgttttatt ttgtaattat gtcgtattat tgcttttgtt ggtgcatacc tctccttgtg
      121 ctaatataag ttaatgatat accgtccgat cgggattaat acttgacatt gcgcgtatca
      181 catatatatt ttaaactaaa gcttttttgt tcatgtggca gtcttaatat ggcattgatt
      241 tatatgttct taagttttgc cgggtttgga tgtattctgt tttaactcta ctcccgtcat
      301 ttttttttgt atcaaactat ataaaacagt gttttgagtt tatttttcgt attaatatgc
      361 cgattatgaa caaagataac tatattgatg tactgttgtc ttctgaattt atatcagttt
      421 aaatcatatt gtcaaaggag ttgataggat gaaaagggag gtaaagactt tattggtaac
      481 tatcctcgca ggttttttgt tcgtccttgc cggggatttt aatataggcc tgattgcaca
      541 ggatgcggca ggcgatgatg ctgcaccggc agcgcggggc ggtgaacgta aaaaactcga
      601 cggccttgtt gttgattgga tgaatgactt tgaagactca gaggattggc gtgctgtttc
      661 gacctgtccc cttggagata caaagattcg taaaatgccg ggaaaaccta ataaagttaa
      721 tgaagacggt acagttgaag atgctaagat cgagcttacc gatgaaaacg gtgttacgca
      781 taaagatgag tatgtgttag gtgcgaaaac gtattttgcg gacagaggtt ttgaccgcgt
      841 tgaagttttt ccgccaaacg aatatattgt ccgtggaaaa gcaaaagagg tacgggtctg
      901 ggttttaggc cgtaagttta gacatacact ttatatcaag cttcgagact atcgtggcaa
      961 gattcataag ataaaagtcg gacgtcttga tttctggggc tggaaagaga tgagtgtaat
     1021 tgtacctgga tggcttccgc aatctgccaa gtacgccatg atggataaaa atcttcattt
     1081 tgtatctttc tttgttgaaa gtgataaatt tgaggtacct ggaacttttt acttttatat
     1141 ggataacttt cgtatagtta cagatctttc agagtttaca ggagatcctg atattaagga
     1201 taactggtaa gataggggcc gcaggccctt catcttgtaa tgaaaatgaa atgtagcact
     1261 gtagctataa atggaggaaa aatgaaacgg cgttatttta agattattat gtttatatta
     1321 tctctttcta tctttggatt gctggttact actgtttatt ctcagctaat aaccaatgtt
     1381 ccaccagata tatcgataga cgagattaag gctcttgttg ttgaagattt tgagtcttct
     1441 cctacttggg ttgtagagac agaacctaag ccatatacaa aaggcggagg cgatgaaaag
     1501 gccaaaaaga atcctgttcc ggttcttgaa ataaaaattg ttgatggcgc tccttctgat
     1561 atgaagcctc aggagtggca ggctgataac aaaggaacca agaaagataa ggttcttgga
     1621 gtaaatttta gatttaaata tccaggacat aatacagtaa agatgacacc tcctgagcca
     1681 ataaatttac caggacgtgc tcgcggtctt tcaatttggg ttcatggacg cggtaacaaa
     1741 tattatcttg agtgctgggt aaaagattat gaaggtagca ctcatatatt gaagttcggt
     1801 gaagtcggtt ttgttggttg gagaccgatg tctgtagaaa ttcctgagtt tatccctcag
     1861 gcagttgatt cgtatccttc gactaaaaca ttgacacttg aaaaatttgt tttaagatct
     1921 gatccaagag aacgtgtagg taatgtattt ttcttttttg atcagctaaa ggtgcttact
     1981 cagacttacg aggttaattt tgacggttca ggcctggagg attctttcga aagttccaaa
     2041 ggaacaagag agacgaaacc tgctcctgcc ggcggtacag aataaaaaaa gttggtgata
     2101 cttaactgaa gtaccatata aagtatccgc ccgcacaagg cggatacttt cgttattaat
     2161 agacaatcat gggagggaaa taatgcagca atttattgat cgtttggagt ctctattaaa
     2221 agaggagtct ttcggtagaa tgggggcatc aagtgttggc atttcccgtt ttaagaattt
     2281 agaatccttt tttaatgaat gtgtgcaggg taatgcgact aaagaaatat atgatgcatg
     2341 tgataaccat cttgatgaaa acccgtcttc ggtgacttct ctttatatta gcggattgat
     2401 agcttttcat tgtaatataa ttgatggaaa gaataatctt attagtttaa ttaaaacttt
     2461 ttcggatctg caaaaatggg ctgttgtgga acatttagca gacaaggtct tggaatatgg
     2521 cgaaaatagc actgctttga aagctcttgc gatgagtctg gaaaaattaa aaagacgtaa
     2581 agaggctgtt ccagtttggg aagagctaat aaaaattaac aggtttgacg cggatatagc
     2641 gcgaaaatta tctcaaacgc ttgaagaaga ggatcaggct aaaagtgttc
//
LOCUS       DAOTGZ010000307         2672 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_3064
            NODE_45356_length_2672_cov_4.051586, whole genome shotgun sequence.
ACCESSION   DAOTGZ010000307 DAOTGZ010000000
VERSION     DAOTGZ010000307.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868088
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 2672)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2672)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:22:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,785
            CDSs (total)                      :: 2,751
            Genes (coding)                    :: 2,732
            CDSs (with protein)               :: 2,732
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 12 of 19
            Pseudo Genes (internal stop)      :: 5 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2672
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_45356_length_2672_cov_4.051586"
                     /isolate="AMR_MDS_3064"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(270..770)
                     /locus_tag="P5123_13285"
     CDS             complement(270..770)
                     /locus_tag="P5123_13285"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRX17283.1"
                     /translation="MFNKYIWLISCISLFFSCGYTPGIRAQNSDGKINKSETVDTDLP
                     DITGTEGLTLTYTKILGMDKKGNYIKEYSFKDNEKELTVTVIPYVKLTGKIVVVGNEP
                     FTMVMLKTSEGSYYINGNEGDAYRDRQTKDVTIEARVEKRKTNNIKTKRSLSRDLLFP
                     IGPSDK"
     gene            complement(792..2273)
                     /locus_tag="P5123_13290"
     CDS             complement(792..2273)
                     /locus_tag="P5123_13290"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRX17284.1"
                     /translation="MKKILIFLIVLLSFLSCDDKSGSSADRQFWVADDTKDYYSSSAG
                     WYQVPANLVYEGKHCEIYLEEDQDISESIIDNIASAFDNRIYDVVTNNFSTPTDIDTN
                     KKVILLLLDIKDGYSGPGDGYIGGYFAPWDLFSESELGMLGFPSHYKTNECEILYIDI
                     DPADPTSAEIKSTIAHEFQHLVNMSQNVIYENGYQMPTWIDEGFSMAAETIYQNVDLL
                     EEREAWYDADPFLTGEFGITKDQSKSIAAGHPLVAWANDDSVYNNYALSHLFFAWLRI
                     HMLANDKDDTFFKEIMTSDNNTLDKVVDVCSSTIGAEIDSNAKIIQRFYMANILGGNG
                     KTTGIESYLDKNRHTGPYSFGSPSTSVDLLPGAAVHFISSSFNSWSPSDEDPAILFSM
                     ANIDTKDADYDEDFGTGDSNNECLVVINSLDEYTGEDSIQNSGTLPNRSYTPYDKSFT
                     IADTRSNRVKLSKLRDTTKKDLPKGAMRRGLEPKGDSTSRFRN"
     gene            complement(2517..2590)
                     /locus_tag="P5123_13295"
     tRNA            complement(2517..2590)
                     /locus_tag="P5123_13295"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(2554..2556),aa:Met,seq:cat)
     gene            complement(2595..2667)
                     /locus_tag="P5123_13300"
     tRNA            complement(2595..2667)
                     /locus_tag="P5123_13300"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(2632..2634),aa:Met,seq:cat)
BASE COUNT          753 a          529 c          531 g          859 t
ORIGIN      
        1 ggaatcgatt ttgtaatgtt attaataagc tgtgaaccgc cttcggttga cataaatgcg
       61 taataggctc cggctccaaa aaatccgact gccgttaata ctgaagatat ccatgtcggg
      121 ttagaaaaag attttattct cataatggaa cctctttaat aacgctttta tgtttcaagc
      181 agtagaaagc ttaagtgaaa gctgccatta tcaggtgaat taggcagcct tttgcttctt
      241 tgattaaacg gttttagtga aaaagttttt tatttatcac tgggtcctat tggaaataaa
      301 agatcacgag agaggctgcg tttggtttta atgttatttg tcttacgttt ttcgactcgg
      361 gcttcaattg taacatcttt agtctgacga tcacggtatg catcgccttc attaccattt
      421 atatagtaac taccttccga tgtttttagc atgaccattg tgaaaggctc attgccgaca
      481 accacaattt tgcccgttaa tttaacatat gggattacgg ttacggtgag ttctttctcg
      541 ttatccttaa aggagtactc tttgatgtag ttaccttttt tatccatacc taaaattttg
      601 gtataggtga gtgtaagacc ctctgttcca gtaatatcgg gcaaatctgt atctacagtt
      661 tctgatttat ttattttccc atcactgttt tgagcccgta ttccgggagt gtatccgcat
      721 gaaaaaaaaa ggctgataca tgatatcagc catatatatt tgttgaacat ctttataaac
      781 tccctcgtaa tctaatttct aaatctggag gtggaatcac cttttggctc aagacctcga
      841 cgcattgcac cttttggaag atcttttttt gtggtatctc tgagtttaga aagttttacc
      901 ctgtttgatc tggtgtcggc aattgtaaat gatttatcat agggagtata cgaacgatta
      961 ggcagggttc cactgttttg aatactatcc tctccggtat actcatctaa gctgtttata
     1021 actacaagac attcgttatt cgaatcacct gttccgaaat cttcgtcata gtctgcatct
     1081 ttagtgtcga tatttgccat agagaaaaga atcgcagggt cttcatcact tggcgaccag
     1141 ctgttaaaac tcgagcttat aaaatgaaca gccgctccgg gaagaagatc aacactcgta
     1201 ctcggcgacc caaaggagta ggggccggta tgtctgtttt tatcaagata gctttcgata
     1261 cccgttgttt tgccgttacc gccaagaatg ttagccatat aaaaccgctg tataattttg
     1321 gcgtttgaat caatttctgc tcctattgtg ctgctgcaca catcaacaac tttatcaagt
     1381 gtgttgttat cactagtcat aatctctttg aagaaagtat cgtctttgtc attagcaagc
     1441 atatgtattc gaagccatgc gaagaaaaga tggcttagcg cgtagttatt ataaacacta
     1501 tcgtcgttag cccaggcaac aagagggtga cctgcggcta tactctttga ctggtctttc
     1561 gttattccaa attcacctgt caaaaacgga tccgcgtcat accaagcttc acgctcttca
     1621 agaaggtcaa cattttgata gattgtttcg gcggccatgg agaagccctc gtcaatccag
     1681 gttggcattt gataaccgtt ttcgtatatt acattttggg acatattgac caggtgctgg
     1741 aactcgtgag cgatagtgct cttgatttct gctgatgtag ggtcagccgg atctatatct
     1801 atatagagaa tttcgcactc attagtcttg tagtgggaag gaaatccaag cattcccagt
     1861 tcactttcag aaaacagatc ccatggggca aaataaccgc ctatgtatcc gtcaccgggg
     1921 ccggagtagc cgtctttgat atccaacaaa agcagaatga ccttcttgtt ggtgtcaatg
     1981 tccgtaggtg ttgagaaatt attcgttaca acatcgtaaa ttctgttatc aaaagcggac
     2041 gctatattat caattatcga ttcagaaata tcctggtcct cttcaagata tatctcgcaa
     2101 tgtttacctt cgtaaacaag atttgccgga acctgatacc acccggcaga gctggagtag
     2161 taatccttgg tatcatcggc tacccagaac tgtctatctg cacttgaacc acttttatcg
     2221 tcacagctta aaaaagataa taaaactatg agaaaaataa gtattttttt catcattttt
     2281 ctccattttt tgaatgaatg aaagatacag ctaaattaga ttttgtcaac agtattagta
     2341 taattaattg ccaatatggt gtaatataga tttacgtaac aatttaactg ataatttatg
     2401 gtaatatcta ataattattt tatgttgata aatattttaa aaataaaaag tagggtggtt
     2461 taactcgaat tatgcattga agctaataac aggtagaaag ggggaagttg aattcttagc
     2521 gagggaggga ctcgaacccc ccaccccacg gatatgagcc gtgtgctcta accaactgag
     2581 ctacctcgcc ttattagcgc ggacaggact tgaacctgtg acctttgggt tatgagccca
     2641 acgagctacc aactgctcca ccgcgcgatc gt
//
LOCUS       DAOTGZ010000308         2632 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_3064
            NODE_46268_length_2632_cov_4.119907, whole genome shotgun sequence.
ACCESSION   DAOTGZ010000308 DAOTGZ010000000
VERSION     DAOTGZ010000308.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868088
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 2632)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2632)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:22:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,785
            CDSs (total)                      :: 2,751
            Genes (coding)                    :: 2,732
            CDSs (with protein)               :: 2,732
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 12 of 19
            Pseudo Genes (internal stop)      :: 5 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2632
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_46268_length_2632_cov_4.119907"
                     /isolate="AMR_MDS_3064"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            271..1143
                     /locus_tag="P5123_13305"
     CDS             271..1143
                     /locus_tag="P5123_13305"
                     /inference="COORDINATES: protein motif:HMM:NF024883.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain-containing protein"
                     /protein_id="HRX17285.1"
                     /translation="MQFIVDGAIEATNANSGAVYLVDNQKLLLVAANPVLQKQYPISV
                     REASLKDHPHINRAVTSGKHVIMRDCTKTILTNAEREIIKLRNLVSNLYLPIHFGNQV
                     IGVLILSSTTDIDIFSNEDILMLQGFADQAAQVINNIRSFETLKESVSGMDSFESDSC
                     KVEDNIRFCEESQRYFLMNLSDNNFDMLTLSDFAGNIQLGCAKNSLKHSLDDIIGKNI
                     LDYVHPEDIDSVKRVISGLALNKVASGQKYRFKRPDGNYVWFESSAIVLRDKFGNPQK
                     LLLYINDLSQSKLS"
     gene            1323..2165
                     /locus_tag="P5123_13310"
     CDS             1323..2165
                     /locus_tag="P5123_13310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013255553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase"
                     /protein_id="HRX17286.1"
                     /translation="MIQENEVKELQKKAFEIRKMTIDAIGHLGVGHIGGAMSIVEVLT
                     LLYYRVLKNIDPKNPQKEGRDKVVLSKGHAGPTLYSILAEKGYFPKEWLYTLNGSDTR
                     LPSHCDMNRTPGIDMTTGSLGTGASAAIGLVLGNRLNGYNDAYTYLIIGDGESQEGQI
                     WEAAMFAAHNKLGNLIAFTDYNKLQIDGYTQDIMSIDDLNSKWNGFGWHVQRVDGHDL
                     RALDAAVKNAQDTKERPSMIIMDTVKSKGFCFGEGIVGNHNMSFDKAKAEEAIKILNK
                     CQGE"
     gene            2168..>2632
                     /locus_tag="P5123_13315"
     CDS             2168..>2632
                     /locus_tag="P5123_13315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020612202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase family protein"
                     /protein_id="HRX17287.1"
                     /translation="MTIDKVLEIADMRSVYTQALIDAAEKNPKIVLLEADLMSAAGTK
                     SFFEKYPDRSFNCGVAEGNMAGIAGGLSASGFTPFAHTFTAFAARKTYDQFYITCNYA
                     LQNVKLVGSDPGITALFNGGTHMSFGDYGLMTTMPKLTVVEPSDRVSMYKLTQ"
BASE COUNT          785 a          464 c          543 g          840 t
ORIGIN      
        1 aaatttgccg atattccgat tgtttttctc tcttctcata ctgaatcaga atttgttcaa
       61 aaaatggagc agattacttc ttacggatat gttgataaga attccggaat cacggttctt
      121 gacgcttcta taaaaatggc ttttaagctt tttgccctta aacaggatat tagaaatatc
      181 aactcttccc ttgttaatac caaccgcaag ttgaatgtgt ttttaagcat ttgtcaacgc
      241 atgacaagca tgttcgaatt ggttccattg atgcagttta ttgtggatgg cgccatcgag
      301 gcaaccaatg ctaattctgg agctgtatat ctggttgata accagaaact tcttcttgtt
      361 gcagcgaatc ctgttttaca aaaacagtat ccaatatcag tacgtgaggc cagccttaag
      421 gaccatccgc atatcaatag ggcggttacc agcggtaagc atgtaataat gcgtgattgt
      481 accaaaacca ttcttactaa tgccgaaaga gaaataatca agttgcgtaa tcttgtttct
      541 aatctttatt tacccataca ttttggaaat caggtcattg gcgtcttgat cctctcttcc
      601 acaactgata tcgatatttt tagtaatgaa gatattttaa tgctgcaggg attcgccgat
      661 caagcagcac aggttataaa caatataaga agttttgaga cacttaaaga aagcgtttcc
      721 ggaatggatt catttgaaag tgattcttgt aaagttgaag acaatatacg gttctgtgag
      781 gagagtcaac gttatttctt aatgaatcta tcggataata attttgatat gttaacatta
      841 tccgattttg ctggtaatat acagcttggt tgtgcaaaaa attcacttaa acattctctg
      901 gatgatatta ttggtaaaaa tatccttgat tatgttcatc ctgaagatat tgattcggtt
      961 aaaagggtca tttcgggttt agcgcttaat aaagttgcct ctgggcaaaa atatcggttc
     1021 aaaagaccgg acggcaatta tgtctggttt gaatcatcag ctattgttct tcgggataaa
     1081 tttggtaatc ctcaaaaact gcttctttac ataaacgacc tctcacaatc aaaactaagt
     1141 tgatttttac cgcctgtatt gataaaatga ttaatgtagt tacataggta ttttctgtat
     1201 ataataccga ttaaacactt atggacgaaa acaaggtgct tttttatttt actttcatta
     1261 ttgacataat ggcagttgtt tttttaactg cttttaaaaa taattttatt taggtatata
     1321 caatgataca ggaaaatgaa gttaaagaat tacaaaaaaa ggcgtttgaa atacgcaaaa
     1381 tgacaataga tgctattggt catctagggg tcggccatat aggtggcgct atgtctattg
     1441 tagaggttct cactcttctt tattatcgtg tgcttaagaa tattgatccc aaaaatcctc
     1501 aaaaagaggg gcgagacaag gttgtccttt cgaaggggca tgccgggcct acactttact
     1561 ccattttggc cgaaaaaggc tactttccaa aagagtggct ctatacactt aacggaagcg
     1621 atacaaggct tccaagtcac tgtgacatga accgtacgcc gggtatcgat atgacaaccg
     1681 gttctcttgg aacaggggct tctgcagcga ttggtcttgt gctgggcaac cgtcttaacg
     1741 gttataatga tgcctatact tatcttataa ttggtgatgg ggagagtcaa gagggacaga
     1801 tatgggaagc tgccatgttt gcagctcata acaagttggg gaatcttatc gcgtttacag
     1861 actataacaa gcttcagatt gacggttata ctcaggatat catgtcaatc gatgatttaa
     1921 attcaaaatg gaacggattt ggatggcacg tacagcgtgt tgacggacat gatttgcggg
     1981 ctcttgacgc ggctgttaaa aatgcccagg ataccaaaga gcgtccaagt atgattatta
     2041 tggataccgt taaatctaaa ggtttctgtt tcggtgaagg tattgttggc aatcataata
     2101 tgtcttttga taaggctaaa gccgaagagg ccataaaaat actcaataag tgccagggag
     2161 aataaccatg acaatagata aagttttaga aatagccgat atgaggtctg tatatactca
     2221 ggctcttata gatgctgctg aaaaaaatcc aaaaattgtg ttgcttgagg ccgatctgat
     2281 gtcggctgcg ggtacaaaat ctttttttga aaaatatcct gatcgttcat ttaactgtgg
     2341 cgttgcagaa ggaaacatgg ctggtattgc tggtggtctt tctgcctcag ggtttactcc
     2401 ctttgctcat acatttacag cttttgcggc tcgtaaaacc tatgatcagt tttacattac
     2461 ctgtaactat gcccttcaaa atgtaaaact ggttggttca gacccaggta ttacagctct
     2521 tttcaatggt ggtactcata tgtcattcgg agactacggt cttatgacta ccatgccaaa
     2581 attgactgtt gttgagcctt cagaccgggt ttcgatgtat aaacttactc ag
//
LOCUS       DAOTGZ010000309         2625 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_3064
            NODE_46458_length_2625_cov_5.420233, whole genome shotgun sequence.
ACCESSION   DAOTGZ010000309 DAOTGZ010000000
VERSION     DAOTGZ010000309.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868088
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 2625)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2625)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:22:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,785
            CDSs (total)                      :: 2,751
            Genes (coding)                    :: 2,732
            CDSs (with protein)               :: 2,732
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 12 of 19
            Pseudo Genes (internal stop)      :: 5 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2625
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_46458_length_2625_cov_5.420233"
                     /isolate="AMR_MDS_3064"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(619..2373)
                     /locus_tag="P5123_13320"
     CDS             complement(619..2373)
                     /locus_tag="P5123_13320"
                     /inference="COORDINATES: protein motif:HMM:NF012245.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein"
                     /protein_id="HRX17288.1"
                     /translation="MSPHNGKKKTQTIMNLKIKTKLAGSYVLILIFFVFVSVFLTRAL
                     ISSGENTEELYQAVRLSRNSMEAKHEFVKFYNILLETKNALNREEYNNVREIVRNKLK
                     SPEESISSNFLKMKDDVIDADIRKTVIELEELHNRWAKLMSDIDNKLANEDQTGSIDL
                     VNSIVTLMNDYIQSFSRLEAFTEKMSEENHIKSRTQIKSARIVSTTVVIFTVILSMII
                     AFMISRNILNSISMFNSIFVKGSSGDLESTYPVQDNSKDEINALGLIFNTFIKKLNEV
                     IREVSEVAHELNSSSEILFETTTGFSTNTQNQAASSEQITATLEEVTAGINNISNNSQ
                     TQQNKLNEVIILIKELSDKIDIMAERISKTRTQSTQITKQAQTGSEALTRMSVSMREI
                     SQSSNEVTDIIQIINDISGEINLLSLNAAIEAARAGDSGKGFAVVADEISKLADQTAS
                     SINQIASLIEKNNNEIANGMHNVDDTISGISKIITGVESIDKMLNDIIQDVNKQQNAN
                     KSVNLSIMELKHLSDEVKFAADEQRTASEEIMKSMNNINQMVQASAGGAEEIAGNVEK
                     LSNMADHQKSKIAFFKFH"
BASE COUNT          737 a          462 c          492 g          934 t
ORIGIN      
        1 ctcaattagg aaaagagaaa acaattgaac ggttactgaa acagcttcgt gatgctggat
       61 taatcgaatt cattggtgat tcgcctaaac cggaggatat tacataactg atattgaccg
      121 atgacgattg aaaatcaagt cactaccagt aggaaagggg cacgatcgta cctcacggtt
      181 taaactattt tagtcttgac ataatcgttg taatcaccta atttatgcta attgagggat
      241 tgtaattccc ataatttatg caaattacaa gaagttaaat cctttaatta gcaataaata
      301 aaggattatt gtgctgaaat tcagagtttt tgctaaattg agttattatg ctaaaaagaa
      361 ttctaaatat ccaacaattt atccgttcca ataaagttat gatactttct ggcgtacata
      421 tgatcagcac gaaatagatt tgattgaaga gagtgacggt aatttgaatg gttatgagtt
      481 caagtactct tcgaaaaagg tgaaggtgcc aaaggattgg cttgatacat acgaaaatgc
      541 aacgcataca accatagata taagttcatt tgaaaacttt ttattgtaat agttcggtga
      601 taatgctctg ataacaaatc aatgaaattt gaagaatgcg attttgcttt tttggtgatc
      661 ggccatgttc gagagttttt ccacgttgcc tgcgatctct tcggctccgc ccgcgcttgc
      721 ttgtaccatc tgattaatat tattcatgga tttcatgatc tcttccgagg cggtacgttg
      781 ctcgtctgct gcaaatttca cttcgtcaga tagatgttta agttccataa tgcttaagtt
      841 tacagatttg ttcgcgtttt gctgtttgtt cacatcctga attatatcgt ttaacatctt
      901 gtcgatagac tcgacacctg taatgatttt acttattcca gaaatggtgt cgtctacatt
      961 atgcattccg tttgctattt cgttgttgtt cttttctatc agtgatgcaa tttgattgat
     1021 actggatgct gtctggtcgg caagtttgga aatctcgtca gcaacaaccg caaagccctt
     1081 tcccgagtca ccggcacgag ccgcttcgat ggccgcgttt aatgataaaa ggtttatttc
     1141 acccgaaata tcattaatga tttgaataat atccgttact tcgtttgaac tttgtgatat
     1201 ttcacgcata cttacgctca ttcgagtcaa cgcttcactt cctgtctgtg cctgtttggt
     1261 aatttgcgtt gattgtgttc tagtttttga gattctttcg gccataatat ctattttgtc
     1321 agataactcc tttattagta taattacttc gttcagtttg ttttgttgag tctgactatt
     1381 gttagagata ttattaattc cggcagtaac ctcttccaga gttgcggtta tctgttcgga
     1441 agaagcggcc tggttttgtg tgttagtgga aaaaccagta gtagtttcga aaagtatttc
     1501 agaagagctg ttgagctcat gagcgacttc ggatacttct ctgattactt cgttcaattt
     1561 ttttatgaag gtattaaaaa ttaatccaag ggcattgatc tcgtcttttg aattatcttg
     1621 aacagggtaa gtgctctcaa gatcgccaga gcttcctttg acaaaaatac tgttaaacat
     1681 tgatattgag tttaaaatgt ttcttgaaat cataaaagct ataatcattg ataaaataac
     1741 cgtaaaaatt acaactgtag ttgatacaat acgggcactt tttatttggg tacggctttt
     1801 tatatgattc tcttccgaca ttttttcggt gaaagcttcc agacgtgaaa agctttgtat
     1861 atagtcattc atcagggtca ctatgctgtt aacaagatca atgcttccgg tctggtcttc
     1921 atttgcaagt ttgttgtcaa tatcggacat taattttgcc catctgttat gcagttcttc
     1981 aagttcaata acagtttttc gtatgtcggc atcaattacg tcatctttca tttttaaaaa
     2041 attgctgcta atgctctctt cggggctttt taacttgttg cgtacaattt ccctgacgtt
     2101 attgtactct tctctgttta acgcattttt tgtttcaagt agtatattat aaaatttcac
     2161 aaattcatgt tttgcttcca ttgaatttct agaaagtcgt actgcttgat acagctcttc
     2221 ggtgttttcc ccggaactta ttaacgccct tgttaaaaaa acacttacaa aaacaaaaaa
     2281 tataagtatt aatacgtaac ttccggccag cttggtcttt atttttaaat tcataatggt
     2341 ttgtgttttt ttcttaccgt tatgtggcga catggatcct ccaaataaag tgccaagttt
     2401 tgcaaaggtg cattcttgtt taatagtata gcagctaatg atattaaaat acaggataaa
     2461 actaaattct tatgtcagat atcgatgagt aaatcggtaa acagaatctt aagctttttt
     2521 ttttatcagg ttgcatctgc gagcagcaag agtcatcttg cgaccagcag aggttgcatc
     2581 tgcgagcagc aagagtcatc ttgcgaccag cagaggttgc atctg
//
LOCUS       DAOTGZ010000310         2623 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_3064
            NODE_46514_length_2623_cov_4.096573, whole genome shotgun sequence.
ACCESSION   DAOTGZ010000310 DAOTGZ010000000
VERSION     DAOTGZ010000310.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868088
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 2623)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2623)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:22:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,785
            CDSs (total)                      :: 2,751
            Genes (coding)                    :: 2,732
            CDSs (with protein)               :: 2,732
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 12 of 19
            Pseudo Genes (internal stop)      :: 5 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2623
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_46514_length_2623_cov_4.096573"
                     /isolate="AMR_MDS_3064"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..616
                     /locus_tag="P5123_13325"
     CDS             <1..616
                     /locus_tag="P5123_13325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013023624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="helicase-related protein"
                     /protein_id="HRX17289.1"
                     /translation="THVFNYHIPFEAEAYVHRIGRTGRAKKDGIAMMLLTPHELRELK
                     RIQEEMGSELELQSIPQKNASQADKERALADMVRSQRATKAGEALLTALTDKTDLATV
                     ANKLALLLEKTLVEDAAIGRSIEEVGQLLQAVGDDVESESKKSSRRRSSSKKSSSRSR
                     SSSGGRGRRRHSDEKIRRGTGRGEVPEPTINSVGGSRIVARKLY"
     gene            771..1703
                     /locus_tag="P5123_13330"
     CDS             771..1703
                     /locus_tag="P5123_13330"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRX17290.1"
                     /translation="MLKNFKSSWKLIKESTKIFFKYPKFLLPLLLNWTFYALIVLYLK
                     FSVNWELFTMGETFLVAFGVILFFALLLTFSCSVLLELIQQLESGRELSLTVAMINSL
                     NKSLLRSLPIVLIWSILWFILIIIQAFLSKKADQNNSNEEFSAENITKTLSGFERMTF
                     PRVLFKTLGKGMRMVIFLILPAIVWDNLSFKKAIKKGYKVFETHLSAFATGLVLTEVA
                     AFIVFFPSAVILQIASESVAPFPEWVWLIVILYIAFAWSYSMYLEQMFSAELYLWNRK
                     WEKEVNLAGQENRAIPALGDVDRPSLLDEDYELV"
     gene            1770..2372
                     /locus_tag="P5123_13335"
     CDS             1770..2372
                     /locus_tag="P5123_13335"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024880.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HRX17291.1"
                     /translation="MNESTRDYYNAYGEEWGKIRYNSFFHEENFRKFIGYLKKGDSIL
                     DIGCANGISVPLFLGIGHSLQYEGLDISETLLTIARNRYPNQSFLLGDIADASSLLEK
                     TYDGFWAPAVLMHVPRELHNDLFHNLASIMKSGAYGYVTLPEAKKKEKEDHDTRTFEF
                     FSIEEARDMFTSRGWKVLESGPLEGSPRNVWNWFIVSLPE"
     gene            complement(2398..>2623)
                     /locus_tag="P5123_13340"
     CDS             complement(2398..>2623)
                     /locus_tag="P5123_13340"
                     /inference="COORDINATES: protein motif:HMM:NF025846.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HRX17292.1"
                     /translation="LYDILSDDPEFDITHLQPDANIIKQVETAAKKIKQNNNPETFAQ
                     DGSVKLKPIFEELGEQIDYDQIKYALLFLE"
BASE COUNT          747 a          484 c          582 g          810 t
ORIGIN      
        1 cacccacgtc tttaactatc acattccatt tgaagccgaa gcctatgtgc accgtattgg
       61 ccgcaccggt cgtgcaaaga aagacggtat cgccatgatg ttgttgacgc cacatgaact
      121 tcgagaattg aaacgcatcc aagaagaaat gggttctgaa ttagaattac aatcgattcc
      181 gcaaaagaac gcatctcagg ctgacaagga acgtgcgctc gccgatatgg tgcgcagtca
      241 gcgtgcgacc aaggcgggtg aggcgctctt gactgcattg accgataaaa ctgaccttgc
      301 cacggtggcg aacaaacttg cgctgcttct tgaaaaaacc ttggttgaag atgccgctat
      361 tggacgatca atcgaagagg taggtcaact gcttcaggct gttggggatg acgttgaatc
      421 agaaagcaag aaatcttccc gaagacgtag ttcgagcaag aaatcttctt cgcgatctcg
      481 atcatcatcg ggtggtcgtg gacgtcgcag acacagcgat gaaaaaattc gtcgtggaac
      541 ggggagggga gaggtgccgg agccgacgat taatagcgtg ggggggtcac gcatcgtcgc
      601 ccggaaactt tattagttcc ataaaatagt cagatatttt tgtcagcttc aaaaaagagc
      661 gctcacggta gggtaactac cgctcgcgct ctttttcctt gcctcctcaa tctctgaact
      721 attttaagaa actagattga tttattaaaa gataagtata gagttggaat atgttaaaaa
      781 actttaaaag tagctggaaa ttaattaaag aatctacaaa aattttcttt aaatacccca
      841 aattcttact ccccttgttg cttaattgga cattttatgc attgattgtt ttatatttaa
      901 agttctctgt taactgggaa ttatttacta tgggtgaaac ttttcttgtc gcctttggag
      961 ttatcctatt ttttgcactg ctcttaactt tctcttgttc cgtattgctt gagcttatac
     1021 agcaattaga atcaggaaga gagttaagct taacagttgc gatgatcaat tctcttaaca
     1081 agagtctatt aagatcgctc ccaattgttt taatctggtc cattttatgg tttattttga
     1141 taataattca agctttttta agtaagaagg ctgatcaaaa taattccaat gaggaattct
     1201 ctgctgagaa tataactaaa acattgtcag gttttgaaag aatgactttt ccaagggtat
     1261 tattcaagac gctagggaaa ggtatgagga tggttatatt tttaatatta ccagcaatag
     1321 tctgggataa cttaagcttt aaaaaggcta tcaaaaaggg atataaggtt tttgaaactc
     1381 atctttctgc ttttgcgact ggccttgttt taacggaagt tgcagctttc attgttttct
     1441 ttccttcggc ggtaatatta cagattgcca gcgagagtgt ggctcctttt ccggaatggg
     1501 tttggctgat tgtgattctt tatattgctt ttgcttggag ttactcaatg tatcttgagc
     1561 aaatgttttc agctgaattg tatttatgga atcgcaaatg ggagaaagaa gtcaatcttg
     1621 ctggacaaga aaatagagct ataccagctt taggtgatgt tgatagacct tcattacttg
     1681 atgaagacta tgagttggta taagcaagga cattttttat tccaaaaaca acagcgcgta
     1741 tttggtgcaa cttttgatat aatcatcaca tgaatgaatc taccagggat tactacaacg
     1801 cttacggtga ggaatgggga aaaatcagat ataattcttt tttccatgaa gagaatttta
     1861 gaaagtttat cgggtatcta aagaagggcg acagtatttt agatattggc tgcgcaaacg
     1921 gaatcagcgt acctttgttt cttggtatcg gacatagttt gcaatacgaa ggtctcgata
     1981 tcagcgagac cttgctaacc attgcccgca atcgttatcc gaatcaatcg tttcttttag
     2041 gcgacattgc cgacgcgtcg tctttacttg aaaaaaccta cgatgggttc tgggctcccg
     2101 cagtattgat gcacgttccg agggagcttc acaatgatct gtttcataac ctcgcatcta
     2161 tcatgaaatc cggcgcttat gggtacgtaa ctttacccga ggcaaaaaag aaagaaaaag
     2221 aagatcacga tacccggacc tttgaatttt ttagcatcga agaagctcgg gatatgttca
     2281 cttcacgagg atggaaagtt ctcgaaagtg gacctctcga aggaagtcct cgaaatgtat
     2341 ggaactggtt tattgtttct ttaccagaat aaaaatcact cttagatcgg gtgattttta
     2401 ctctaaaaac aacagcgcgt atttgatttg gtcgtaatcg atctgctcgc caagttcctc
     2461 gaaaattggt tttaacttca ccgatccgtc ttgagcgaat gtttcagggt tgttgttttg
     2521 tttgattttt ttggcggcag tttcgacttg tttaatgata ttcgcatctg gctgcaggtg
     2581 ggtgatatca aattcaggat catctgacaa aatgtcgtaa agg
//
LOCUS       DAOTGZ010000311         2618 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_3064
            NODE_46645_length_2618_cov_2.863831, whole genome shotgun sequence.
ACCESSION   DAOTGZ010000311 DAOTGZ010000000
VERSION     DAOTGZ010000311.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868088
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 2618)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2618)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:22:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,785
            CDSs (total)                      :: 2,751
            Genes (coding)                    :: 2,732
            CDSs (with protein)               :: 2,732
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 12 of 19
            Pseudo Genes (internal stop)      :: 5 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2618
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_46645_length_2618_cov_2.863831"
                     /isolate="AMR_MDS_3064"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..606)
                     /locus_tag="P5123_13345"
     CDS             complement(<1..606)
                     /locus_tag="P5123_13345"
                     /inference="COORDINATES: protein motif:HMM:NF013740.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HRX17293.1"
                     /translation="MEKNSNVLLILLVFVVFYLLATLQSILIPLTLALLFALMFQPLI
                     IFLDKKRVPGALIVPILSILTLTAIFVVINIVVSSVSQIFSAKDFFAERFSYKMTLLI
                     DIIESHTQSPLNIKSIVEQALTHFDTSLISSTAGSIVGRLSSFAGSFVMFSIYFIVLL
                     SGMANYKRYLIHVGGKKSAKLLISNYETIQKSIFSYLTIKTF"
     gene            complement(752..>2618)
                     /locus_tag="P5123_13350"
     CDS             complement(752..>2618)
                     /locus_tag="P5123_13350"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HRX17294.1"
                     /translation="MLQPYLFTNRDEYLEELDTHLEGVKNSLKADIVYIMDTNGLVIA
                     SSSAVGSDPLTGNNYGFRPYFRKALRGEVAVYPGLGVTTDQRGIYYSAPLRERGGKII
                     AVVVIKVNVNRLEKELYKYNDPTFIITKEQVIFASNRPELLYHYILPINESTLENIYR
                     SNQFASTELRPFIHITNDDSITFKNRDYHAQMVSLKNTGWKLIKLNSDRSLANSKYCK
                     KIYAIILAFLLFMLALIYYIALYLFNRKEKELNADIKLYKNLFSLGPDAVCVTDIEGN
                     ILSHNEAFSQLFGYEPKTIESMNIRDFYADPETDRPRLLYRLDLKKSLRNYILVMKTK
                     DGKLLRTSNSLIRIDHKGNQVIESVIRDISESEIKKQQLRIAQKREMIGIMSSGLAHD
                     FNNILAAINASASLIRYSNTNNQPGDIEDIVLTIENAVEKGSTLIRRISSLARHEESQ
                     YSLVDLTKLLNEIHDIFRKTVPSDIKTKICINTGKSRIKGDPVQIEQVILNILINASH
                     SMSIMKHNNQTRGGTITTTLDKVEKKDLTDLFDKCESDSYICICISDTGVGIPSDLQE
                     RVFEPFFTTKDKTKGSGLGLSMVFDIIKQHGGLIRFESEENRGTTFFIYLPSADI"
BASE COUNT          781 a          446 c          551 g          840 t
ORIGIN      
        1 aaacgttttt attgtgagat acgagaatat agatttttga atggtttcat aattgctgat
       61 taataacttg gcgctttttt tgccgccgac atgaataaga taacgtttgt aatttgccat
      121 gccagataga agcacgataa aatagatgga gaacattaca aacgatcccg cgaacgagct
      181 aagacgcccg acaatagaac cggcggtact tgatatcagg ctggtatcaa agtgggttaa
      241 agcctgctcg acaatgcttt ttatgtttaa gggggattgt gtatggcttt cgattatatc
      301 tattagtagt gtcatcttgt aggagaaccg ctcagcaaaa aaatcctttg ccgaaaatat
      361 ttgcgatact gatgaaacca caatgttaat aacaacaaaa atagcagtta gtgtcagtat
      421 tgataaaatg ggaactatca gggctccagg aactcttttt ttatccagaa aaataatgag
      481 tggttgaaac attaatgcaa ataaaagtgc cagcgtaaga ggtatcagta tggattgcag
      541 tgttgccaac agatagaaga caacaaatac aagtagaata agcagaacgt tcgaattttt
      601 ttccatgatt tttaccggct cctatagtcg cggttattta aatagcagat gttttttatg
      661 tcaagataag cgtgtttaag agtagtaaaa aacttcgttt ataaatattt gatagatcaa
      721 ttgacaaaaa aaaagattta tctaacattt tttatatatc tgcagatggc agatatataa
      781 aaaaagtagt gcccctgttt tcttcggatt caaatctgat aagaccccca tgctgtttga
      841 ttatatcaaa taccatggag aggcctaacc cgctgccctt cgttttatct tttgttgtaa
      901 aaaaaggttc aaaaacacgt tcttgaaggt cagacggtat gccgacgcct gtatcactta
      961 tgcaaataca aatatagctg tcactttcac atttgtcaaa aagatctgtt aaatcttttt
     1021 tttcgacttt gtcaagagtc gtagtaatcg taccgcctct tgtctgatta ttatgtttca
     1081 taatagacat agagtgggac gcattaatca ggatgtttaa tattacctgt tcgatctgga
     1141 caggatcgcc tttaatccga gattttccgg tattgatgca tattttggtt ttaatatcag
     1201 aaggaactgt ttttctgaat atatcatgta tttcgttcag taatttggtg agatctacca
     1261 gactgtattg gctctcttca tggcgggcca gagaagatat tcttctgata agggtggagc
     1321 ctttttcgac tgcgttttca attgttaaca cgatatcttc tatgtcgcca ggttggttgt
     1381 tggtatttga ataccgtata agagaagctg aagcattaat agctgccaga atattattaa
     1441 agtcgtgtgc aagaccgctg gacattattc cgatcatttc acgtttttga gcgattctaa
     1501 gctgttgctt ctttatttcg gactcagaaa tatcccgtat taccgattca ataacctggt
     1561 tgcccttgtg atcaattcga ataagtgaat tagaagtgcg aagtagtttc ccgtcctttg
     1621 ttttcattac gagtatatag ttgcgaagtg atttttttaa atcaagacgg taaagaaggc
     1681 gagggcggtc tgtctcggga tcggcataga aatcacgaat attcattgat tcaatagttt
     1741 tcggctcata accgaaaagc tgtgaaaacg cctcattatg tgatagtatg ttgccttcaa
     1801 tatcagtaac gcaaactgcg tcagggccaa gcgaaaaaag gttcttatat agcttgatgt
     1861 ctgcgttaag ttccttttct tttctgttaa acaggtaaag tgcaatataa tagattagcg
     1921 cgagcatgaa taaaagaaag gcaagtatta tggcgtaaat ttttttacaa tatttagagt
     1981 tcgcgagaga acgatcggaa tttagtttaa tcaatttcca gccggtgttt tttaaagaaa
     2041 ccatttgtgc gtgatagtcg cgatttttaa aagtgatgga atcgtcgtta gtgatatgaa
     2101 taaacgggcg taactcggtt gacgcaaact ggttggagcg gtagatattt tcgagagtgg
     2161 attcgttaat aggcagtata tagtgataaa gaagttccgg gcggtttgag gcaaatatca
     2221 cctgctcttt ggttattata aatgtgggat cattgtattt gtataattcc ttctccagcc
     2281 ggtttacatt aacctttatt acaacaacgg caatgatttt acctccgcgc tctcttaaag
     2341 gagcgctgta atagatacct ctttgatctg tagttacgcc aagaccaggg taaaccgcta
     2401 cttcacctcg tagagccttt ctaaaatagg ggcgaaaacc atagttgttg ccggttagtg
     2461 gatccgaacc gacagcgctg cttgacgcaa ttaccaggcc attggtatcc attatgtaaa
     2521 caatatcagc tttgaggctg tttttgactc cttctaggtg ggtatctagc tcttcaagat
     2581 attcatcccg gttagtaaaa aggtaaggct gtagcata
//
LOCUS       DAOTGZ010000312         2596 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_3064
            NODE_47172_length_2596_cov_4.351830, whole genome shotgun sequence.
ACCESSION   DAOTGZ010000312 DAOTGZ010000000
VERSION     DAOTGZ010000312.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868088
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 2596)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2596)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:22:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,785
            CDSs (total)                      :: 2,751
            Genes (coding)                    :: 2,732
            CDSs (with protein)               :: 2,732
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 12 of 19
            Pseudo Genes (internal stop)      :: 5 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2596
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_47172_length_2596_cov_4.351830"
                     /isolate="AMR_MDS_3064"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..281)
                     /locus_tag="P5123_13355"
     CDS             complement(<1..281)
                     /locus_tag="P5123_13355"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HRX17295.1"
                     /translation="MGNNVKKVKKLPKQNETVELSQLLKQIKSLEEHNRRLDIENNRL
                     REDKKIISTDRDYYKKIIENAPAGFLILDSSGKVLFANRILLKMTGTKK"
     gene            complement(590..1315)
                     /locus_tag="P5123_13360"
     CDS             complement(590..1315)
                     /locus_tag="P5123_13360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020252671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0017004 - cytochrome complex assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c biogenesis protein CcdA"
                     /protein_id="HRX17296.1"
                     /translation="MVESLFISLTSLMYGTTIAALSAAFLWGVISVILSPCHLGSIPL
                     IVAFINGQRRVGIKKSFLISVIFSSGILISIGLIGLVTGLMGNILGDIGKTGTIIVII
                     VFVLIGLHFIGIIPLPDFFSGDKSKITKKRSLLSAFFLGLLFGCALGPCTFAFMAPVL
                     AVVFNSASEDIGFALALVAVYAVGHSLVFIFFGTFSGAAKKLLKWEERSSATKVIRII
                     KIIIGVIMIFTALYMGYKYLGLF"
     gene            complement(1317..1820)
                     /locus_tag="P5123_13365"
     CDS             complement(1317..1820)
                     /locus_tag="P5123_13365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017950752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin family protein"
                     /protein_id="HRX17297.1"
                     /translation="MFHNITLPMKFIITLCFVTIMVIAIFGSCSRPSDDGEVTLSSVA
                     KVEEPAQEKETTDTAKKDDVSENKSPSGKLPIVTFIELGSVNCVPCKMMQPVMDQVKA
                     TYGDKVDVVFYDVWTEEERTMAEKYRIRAIPTQVFLDANGNEYYRHTGFFPFEELDKV
                     LKAGGVR"
     gene            complement(1820..2200)
                     /locus_tag="P5123_13370"
     CDS             complement(1820..2200)
                     /locus_tag="P5123_13370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013969295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative zinc-binding protein"
                     /protein_id="HRX17298.1"
                     /translation="MSESCSCSCGVSCKPLLYACSGAANTGYLSDAVARKIMGNGNGS
                     MTCLAAVGAKLSGFVETAKSSNNIVIDGCPTACGKKIFDTHNLDYKHIILTDFDVQKG
                     KTEITPDLIEKITIKVESKIQGVT"
     gene            complement(2214..2441)
                     /locus_tag="P5123_13375"
     CDS             complement(2214..2441)
                     /locus_tag="P5123_13375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012102109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015036 - disulfide oxidoreductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin family protein"
                     /protein_id="HRX17299.1"
                     /translation="MVIKILGSGCPKCKQLEEETRKAVSESGTEADIQKVSDMDQIMD
                     YGVMMTPALVIDEEVKSVGKVLKAGEIKKFL"
BASE COUNT          771 a          503 c          467 g          855 t
ORIGIN      
        1 tatttttttg taccggtcat ttttagcagg atgcggttag caaatagaac tttccctgaa
       61 gaatccagta taagaaatcc cgccggagca ttttcgataa tttttttgta ataatctcta
      121 tcagtggata tgattttttt atcttcgcgt aaacggttgt tttcgatatc aagcctgcga
      181 ttatgctctt cgagtgattt tatctgcttc agcaattgtg atagttccac tgtttcgttt
      241 tgctttggca atttttttac tttttttacg ttattaccca ttagcgaccg ttaattgtta
      301 ataatcctgt taataaaaaa ggtatcagat acattatatc actatgattt gaaactcata
      361 ggagaaaata atacacttta tataataatt tcaagaaaaa tgcgatcttc atgaaaatat
      421 ttagtcataa tttgtaaaat gattttaaat ttcggcaaaa aataaattat gcctatgtat
      481 aattgaacga agacattttt ttgggcagat ataatctgcc caaataattt tataacagaa
      541 attttataag tcaatattag aatattgttt aatgaggtat tgttactttt taaaagagtc
      601 ccaggtattt ataacccata taaagagctg taaaaatcat tatcaccccg attattatct
      661 tgatgattct gattactttg gttgctgatg atctctcttc ccattttagc agttttttgg
      721 cagctccgct aaatgtgcca aaaaagataa agaccaatga gtggccaaca gcgtacacgg
      781 cgacaagggc aagggcgaat ccaatgtctt cagaggccga gttgaaaact actgcaagta
      841 caggagccat aaaggcaaat gtgcagggac caagtgcgca gccgaaaagc aggccaagaa
      901 aaaatgcaga caagagtgat cttttctttg ttattttgga tttatcaccc gagaaaaaat
      961 caggaagcgg aatgatgccg atgaagtgaa gtccaattag cacaaaaacg ataattacga
     1021 ttatggtgcc ggttttaccg atatcaccca gaatgtttcc cattagtcct gtaacaagac
     1081 cgatcaaccc tattgagata agtatcccag aactgaaaat cacagaaata agaaaagatt
     1141 ttttgatacc gacccggcgt tggccgttaa taaatgcgac aatcaaaggt attgatccca
     1201 ggtgacaggg gctcaaaata acgctgatta ccccccataa aaatgccgcg ctgagggcgg
     1261 caattgtggt tccatacatc aatgaagtta atgatataaa tagtgattct accatatcag
     1321 cgtaccccgc cggcctttag taccttatct agctcttcaa acgggaagaa gcctgtgtgt
     1381 ctgtagtact catttccatt cgcatccaaa aagacctggg taggtatagc acgtattctg
     1441 tatttttcgg ccattgtgcg ctcttcttcg gtccatacat cataaaaaac aacatccacc
     1501 ttatcaccgt aagttgcttt aacttgatcc atcactggtt gcatcatttt gcaaggaaca
     1561 cagtttaccg aaccgagctc tataaaggta acaatgggta acttccccga aggtgatttg
     1621 ttttcagaga catcatcctt tttcgctgta tccgtcgtct ctttctcttg tgctggttcc
     1681 tcaactttag caacagatga aagcgtaact tctccatcgt ctgatggtct agaacatgaa
     1741 ccaaatattg ctataaccat tattgttaca aagcagagag taattataaa tttcataggt
     1801 agcgttatgt tgtgaaacat caagtgactc cttggatttt gctttcgact tttattgtta
     1861 ttttttcaat aagatccggg gtaatttctg ttttgccttt ttgtacatca aagtcggtta
     1921 gtataatatg tttgtaatca agattatgcg tatcaaatat tttttttccg catgcggttg
     1981 ggcatccatc gatgacaatg ttgttagagc ttttagcagt ctcaacgaat ccggaaagtt
     2041 tcgcgccaac ggcagcaagg caggtcatgc tgccgtttcc atttcccata atttttcttg
     2101 ccacagcatc agaaagataa cctgtgtttg ccgctcccga gcaggcatat aaaagtggtt
     2161 tgcagcttac gccgcaagag catgaacatg attctgacat aatgattctc cttttaaaga
     2221 aattttttga tttcgcctgc cttaagtact ttgccaacag attttacttc ttcatcaata
     2281 acaagtgccg gtgtcatcat tacaccataa tccattatct gatccatgtc ggatactttc
     2341 tgaatatcag cttcggtacc cgattcgctt accgccttac gcgtttcttc ttcaagctgt
     2401 ttgcatttcg ggcatccaga gcccagaatt ttaataacca tatttttttc tccttttgtt
     2461 aaatataata taaaaatttc gataatattc tatttgatga tttatgctct ttccgctttt
     2521 ggctgtaaaa atataattaa ttaaaatcca aaaagtttta aaactgcgtt tatgtgacgt
     2581 tatgtttacg ctgttc
//
LOCUS       DAOTGZ010000313         2556 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_3064
            NODE_48105_length_2556_cov_4.109156, whole genome shotgun sequence.
ACCESSION   DAOTGZ010000313 DAOTGZ010000000
VERSION     DAOTGZ010000313.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868088
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 2556)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2556)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:22:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,785
            CDSs (total)                      :: 2,751
            Genes (coding)                    :: 2,732
            CDSs (with protein)               :: 2,732
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 12 of 19
            Pseudo Genes (internal stop)      :: 5 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2556
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_48105_length_2556_cov_4.109156"
                     /isolate="AMR_MDS_3064"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..638)
                     /locus_tag="P5123_13380"
     CDS             complement(<1..638)
                     /locus_tag="P5123_13380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002659254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trypsin-like peptidase domain-containing
                     protein"
                     /protein_id="HRX17300.1"
                     /translation="MKKFFMILVIVLLAICMPIFDIFSSSLEALDKDLEAIATKVLPS
                     VVSTMGETDDAGSRGIFGRSRPGGSRKSMGSGVIIDKRGYIVTNYHVIRELKEINIVL
                     FDKKEYRCDIVGSDPATDIAVLKIKGKVPSSLPVIEMADSDKLAPGQIAIAVGNSFGL
                     SHTVTMGIVSAVNRDNIGLLDFENLIQTDAAINPGNSGGALVNVKGQLIGINT"
     gene            complement(762..1856)
                     /locus_tag="P5123_13385"
     CDS             complement(762..1856)
                     /locus_tag="P5123_13385"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRX17301.1"
                     /translation="MNNNIKTNIKIVLTGCIVSCFALVSCTATRSSFKQVKYESDIKK
                     IIYYGTLAGNSHNTQPWKVKIIDDKTIEIYADLSRKLNVVDETARGLYISLGAFIENC
                     VLSAGALGYHADVMCPTKISNDYMVSRITIKKAKPTDFDIANIKERSTLRTPFKTDPI
                     SKKDLTYLIDHDNDTILFFEAESEMGKYISQQNLLAYTQQSHDTRAKEELASWIRFAN
                     SDVKKYRDGLTTAGMGIKGFSALFVRNFFSPEDSKSDSFVEAGIKKAEDQLSNHAGWI
                     AIKQPGSTPIDWMEAGRVYQRVHLKCRSRMIGFHPFNAVIEENNYERKFIKKINIKGK
                     VHLLARIGYVDSYPEPVSVRRTVNDILIEE"
     gene            complement(1981..>2556)
                     /locus_tag="P5123_13390"
     CDS             complement(1981..>2556)
                     /locus_tag="P5123_13390"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRX17302.1"
                     /translation="LRINEPHGDTSRKLTGDDLDGIVYSAGGGAAFQFDLAAIVPGEW
                     NHLIFRTYHTCFYRGLTSADNEQSWMFENDEGENRNGYNYYGNYLIGYQMPLKFSFVG
                     VLFEEELRLYETENGSNWGDDLSRWTISPLAVFDFTDKISATCLVQFRTERNFIDGTT
                     DNDFYQTRKIDSDDERDIEFYRAVFIINYKL"
BASE COUNT          771 a          555 c          421 g          809 t
ORIGIN      
        1 gtgttaattc caataagctg cccctttacg ttaacaagag ctcccccaga atttcccgga
       61 tttatcgcag catcggtctg aatcagattt tcaaaatcaa gcaaaccgat attgtcacga
      121 ttaaccgcac ttacgattcc cattgttaca gtatgggaca gcccaaagga gttcccgact
      181 gctatggcaa tctgcccagg agcaagcttg tcggaatctg ccatttcaat tacgggcaat
      241 gaagagggaa ccttaccttt tattttaagc acagcgatat cggtcgcagg gtcagagcct
      301 acaatatcac agcgatactc ttttttatcg aataaaacaa tatttatctc ttttaattca
      361 cgaataacat gataatttgt tacaatgtat cctcttttat cgattataac acccgacccc
      421 atggattttc gcgatccacc aggacgagac cttccaaaga tacctcttga accagcgtca
      481 tctgtctcac ccatggtact tacaacggaa ggcaaaacct ttgtggcaat tgcttctaga
      541 tccttgtcta aggcctcaag tgaagatgag aaaatgtcaa aaatcggcat acatatcgca
      601 aggagaacta taacgagtat cataaaaaat tttttcatgt taaacttccg tctttatatc
      661 atttttaaac atactaataa cctgattatc gactgatact gtaaagctct aaaacagata
      721 atgcgccttc aataataatc cgataattat tactttcatt actactcctc aattagtata
      781 tcattgactg ttcgacgaac actcacaggt tctggataag aatccacgta gccaatgcga
      841 gcaagaagat gaactttgcc ctttatattt attttcttga taaactttct ttcataatta
      901 ttctcttcta ttactgcgtt aaagggatga aacccaatca tgcgggatcg gcatttaaga
      961 tgaacacgct gatacactct ccccgcttcc atccagtcta ttggtgtaga accgggctgt
     1021 tttattgcga tccaacccgc gtgattactc aactgatcct ctgccttctt gattccagct
     1081 tcaacaaatg agtcactctt tgaatcttct ggtgaaaaga aattacgcac aaaaagggca
     1141 ctaaaacctt taatacccat acccgccgtg gtcaagccat cccgatattt cttaacatct
     1201 gaatttgcaa acctgatcca gcttgcaagc tcttctttgg cccttgtgtc gtgcgattgc
     1261 tgtgtataag caagaagatt ctgctgcgat atatattttc ccatttctga ttcggcttca
     1321 aagaagagta ttgtatcatt atcatggtct atcagatagg taagatcttt tttacttatc
     1381 ggatctgttt taaaaggagt tcgcaacgtg gatcgttcct tgatattggc gatatcaaaa
     1441 tctgtaggct ttgccttttt aatggttatt cgcgagacca tataatcatt ggaaattttt
     1501 gtcggacaca taacatcggc atggtatccc agagcaccag cagacaaaac acaattttct
     1561 ataaaagcgc ctaatgagat atagagccca cgcgcagttt catccacaac attaagctta
     1621 cgtgataaat cggcataaat ttcgatagtt ttatcatcga ttatttttac tttccagggt
     1681 tgagtgttat gcgagttacc tgcaagggtt ccatagtaaa ttattttttt tatatctgat
     1741 tcatacttta cctgtttaaa agatgatcgt gtagcagtac aagagactaa tgcgaaacaa
     1801 gagacgatac atcctgttaa gactattttt atatttgttt ttatattatt gttcatgatt
     1861 ttcctctttg aataattttg ataaaataac catataggtt tttatcattc aactatgata
     1921 agtattctta taaaattgct tcaacttatg gtgattaaaa tttccataat taccacttaa
     1981 tcaaagcttg tagtttataa taaaaactgc tcgataaaat tcaatatcgc gttcatcatc
     2041 tgaatctatt ttacgggtct ggtaaaaatc gttatcagtg gttccatcaa taaaattacg
     2101 ttcggttcta aactgtacca gacatgttgc agaaatttta tccgtgaaat cgaacacagc
     2161 aagtgggctt attgtccatc tcgataggtc atcaccccaa ttagaaccgt tttccgtctc
     2221 atagagtctg agttcctcct caaacaaaac acccacaaaa ctgaatttta aaggcatctg
     2281 atatccgatt aaataatttc cataataatt gtatccgttt ctgttttctc cctcgtcgtt
     2341 ctcgaacatc catgactgct cattatctgc cgaagtcaag ccacggtaaa aacaagtatg
     2401 ataggttcta aaaataaggt ggttccactc tcccggtaca atcgccgcca gatcaaactg
     2461 aaaagcagca ccgccaccag cactgtaaac aattccgtcc aaatcatcgc ctgtcagctt
     2521 tcttgaagta tcaccatggg gttcattaat ccttaa
//
LOCUS       DAOTGZ010000314         2539 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_3064
            NODE_48521_length_2539_cov_4.079710, whole genome shotgun sequence.
ACCESSION   DAOTGZ010000314 DAOTGZ010000000
VERSION     DAOTGZ010000314.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868088
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 2539)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2539)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:22:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,785
            CDSs (total)                      :: 2,751
            Genes (coding)                    :: 2,732
            CDSs (with protein)               :: 2,732
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 12 of 19
            Pseudo Genes (internal stop)      :: 5 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2539
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_48521_length_2539_cov_4.079710"
                     /isolate="AMR_MDS_3064"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..797
                     /locus_tag="P5123_13395"
     CDS             <1..797
                     /locus_tag="P5123_13395"
                     /inference="COORDINATES: protein motif:HMM:NF018534.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="penicillin-binding protein 1C"
                     /protein_id="HRX17303.1"
                     /translation="YSQKIAWKTGTSLGHRDAWAIGVSGKYTVGVWCGNAYGEAVSGM
                     TGLQAASPILFDIFGSLPTSEWIISPNTDLKYIDICDVSGFLPNEHCNITQTRVPVSA
                     NPEKQCRYHRLIHLDTSRKYRVTNRCESVNNMVHESMFVLPPVEEFYYRKTNINHVKL
                     PPLREDCMIQQENSSVKQISLIYPSANTKVFVPTDISGKKSELIFKAAHIEDDAIIYW
                     HLDDAYLGSTTEFHNMNVQPDPGKHKLTLVDSKGYSISTIFEVIGE"
     gene            complement(872..1126)
                     /locus_tag="P5123_13400"
     CDS             complement(872..1126)
                     /locus_tag="P5123_13400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020207712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GlsB/YeaQ/YmgE family stress response membrane
                     protein"
                     /protein_id="HRX17304.1"
                     /translation="MSTTSLIIFLLIGLVAGWLAGMIYKGMSFGLVGNLVVGVVGAFV
                     GGFLFNLLEISIGGGIVSAIISALVGALILLFVINLVLKK"
     gene            1504..>2539
                     /locus_tag="P5123_13405"
     CDS             1504..>2539
                     /locus_tag="P5123_13405"
                     /inference="COORDINATES: protein motif:HMM:NF019236.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1565 domain-containing protein"
                     /protein_id="HRX17305.1"
                     /translation="MKKITLIYSIFLFFITGCGTNLYDLADSYKTETEEEDSDTNGAE
                     VEEEDPDINGSPSILVRAVYLSAVVSSNSILPATPVNSAGNTDLIFQIENTGSTVLTV
                     YSITAGSGSFECMEPMPFAVEPGAEHQFTLRITSPGTGSILSQLRVESNDPETPAFDF
                     TINIIAHSSPIYVSADADETGSGTYSNPYRSLSEAIGNVTSDGTIIIGSGNYDGSFVI
                     NLPMKLLGNYRYNGSGWVMVPEDETEGMPVIRNSGPSPDSSTVHIATSLLSSEISIIS
                     LDINTPNASANASSILVDSQTAASGEIVIRNCNISNPDNYNANSSSHAIRLTSGNQNG
                     TVSITDSFITG"
BASE COUNT          812 a          541 c          509 g          677 t
ORIGIN      
        1 tttattcaca aaagattgcc tggaagaccg gaacaagtct tggacaccgc gacgcctggg
       61 caattggagt cagcggaaaa tacaccgtag gtgtctggtg cggaaacgcc tatggtgagg
      121 ccgtttccgg aatgaccgga ctacaggccg cgtcgcctat tctttttgat atttttggaa
      181 gccttccaac gagtgagtgg attattagtc caaacaccga tcttaagtat attgatattt
      241 gtgacgtatc gggttttctt ccgaacgaac attgtaacat tacccaaacc agggtgccgg
      301 tttcggctaa tccggaaaaa cagtgtcgtt atcaccggct tattcatctt gatacaagca
      361 gaaaataccg tgttacaaac agatgtgaat cggtaaacaa tatggtgcat gaatcaatgt
      421 ttgttttgcc gcctgtggag gagttttatt accgcaagac caatattaat catgttaaac
      481 tgcctcccct tcgtgaagac tgcatgatac agcaagaaaa cagcagcgtt aaacagataa
      541 gccttatata cccatccgca aacacaaagg tttttgtacc aacagatatt tcgggaaaaa
      601 aaagcgagct gatattcaaa gcagcacata tcgaagatga cgcaattatt tactggcatc
      661 tggatgatgc atatcttgga agtacaaccg aatttcacaa catgaacgta caacccgacc
      721 ctggaaaaca taaactgaca cttgtggatt cgaaaggtta ttcaataagc acgatttttg
      781 aagttatagg ggagtagcta gaatattttc cgcgtttcat tagaacatta aagtacaaac
      841 cgtataaaac gcggaaattt tctggcaaag tttacttctt aagaacgaga ttaattacaa
      901 atagaagtat aagcgcccca acaagagcag aaattattgc cgagactata ccgccaccaa
      961 tagagatttc aagaagattg aaaagaaaac cgcctacaaa agcacccaca acacctacca
     1021 caaggtttcc aacaagacca aagctcatac ctttgtagat catgcctgca agccaaccgg
     1081 ccacaagacc gattaaaaga aaaataatta atgaagttgt actcataatt cctccggata
     1141 tttgtaaaga ttcactccaa accgccgtat tcaaaatgca taaacgcgga tatatatgca
     1201 gcatactgct ttatgataca taattcaagt ttttgtctaa aatctttctg tttaaccatc
     1261 gtgatggcgc tttatcccgt cgcaaaacag gacaaaacag gtctcgatgc aaggcacatt
     1321 tactcattta caatagatat catcgaatat caaaaatcga ttaaattatt aaaaaatatc
     1381 gtaattttga tgattttaag tatatttcta ttaaattaca tcataccttt tgcattagag
     1441 ctgactgtaa agaaggtaat ttcctgttta tgcccctgct tgaaaacgaa tagggagctt
     1501 aatgtgaaaa agataacact gatttatagt atattccttt tttttattac tggttgcggc
     1561 actaaccttt acgatctggc agatagttac aagacagaaa cagaggaaga agattccgat
     1621 acaaacggag cagaagttga ggaagaagat cctgacataa acggcagtcc gtcaattctt
     1681 gtcagggcag tttatctgtc agcagttgta tccagcaaca gtatactacc ggccactcct
     1741 gttaattcag caggcaatac cgacctgatt ttccagatag aaaataccgg gagcactgtc
     1801 cttacggtat acagcattac tgccgggtcg ggttctttcg aatgtatgga accaatgcct
     1861 tttgcagttg aaccaggcgc ggaacaccag tttacactta gaataacttc acccgggaca
     1921 ggaagcattc tgtctcaact gcgtgtcgaa agcaatgatc ccgaaacacc ggcttttgac
     1981 tttactatta atattattgc tcattcatct ccgatttatg tttcggccga tgctgatgaa
     2041 acaggcagcg gaacatacag caatccctac cggtctcttt ctgaggcgat aggcaacgtg
     2101 acttcggatg gaacaataat aattggcagc ggaaattatg atggatcttt tgttattaac
     2161 ctccccatga agcttttagg caattaccgc tacaacggtt cgggctgggt aatggtaccg
     2221 gaagatgaaa cggagggaat gccggttata cgcaacagtg gaccaagtcc cgacagcagc
     2281 acagtgcata tagcaacatc attgctctct tctgaaatat ccataatctc tcttgatata
     2341 aatacgccga atgcctctgc taacgcatcg tctattcttg ttgattcgca gactgcagct
     2401 tccggtgaaa ttgttattag aaactgcaac ataagcaacc ctgacaatta taacgctaac
     2461 agcagctcac acgcgataag gctgacaagc ggaaaccaaa atggtactgt ttcgatcacc
     2521 gattcgttta ttacgggag
//
LOCUS       DAOTGZ010000315         2533 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_3064
            NODE_48667_length_2533_cov_5.800242, whole genome shotgun sequence.
ACCESSION   DAOTGZ010000315 DAOTGZ010000000
VERSION     DAOTGZ010000315.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868088
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 2533)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2533)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:22:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,785
            CDSs (total)                      :: 2,751
            Genes (coding)                    :: 2,732
            CDSs (with protein)               :: 2,732
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 12 of 19
            Pseudo Genes (internal stop)      :: 5 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2533
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_48667_length_2533_cov_5.800242"
                     /isolate="AMR_MDS_3064"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..504
                     /locus_tag="P5123_13410"
     CDS             <1..504
                     /locus_tag="P5123_13410"
                     /inference="COORDINATES: protein motif:HMM:NF015817.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DbpA RNA binding domain-containing protein"
                     /protein_id="HRX17306.1"
                     /translation="SRDRGSGFGRRDSSSRFSSDKRRTGPGRSRNARSEGSANSRPPY
                     EKKRKPDESYSRMYMNAGRKDGIGVVDLISLLKEKTRVRNIEVGKVDIMDKLTFFEVL
                     KKDEKLVVSSMKNTVLGSRAVRVEPADENKTVTAKGKSSGKSKVERSSKPKGASNKPA
                     GSSRKKD"
     gene            complement(553..1986)
                     /locus_tag="P5123_13415"
     CDS             complement(553..1986)
                     /locus_tag="P5123_13415"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0052621 - diguanylate cyclase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sensor domain-containing diguanylate cyclase"
                     /protein_id="HRX17307.1"
                     /translation="MIRTKLYISLTIIILMFGFILTTLSSFFVSRHSLRSQITSNEIP
                     LTTGTIYSEIKSDLLASKYISSAMANDIFLKDWLISGEKELSRVENYLKQYVRYLGLS
                     SAFVISENSKKYYFQAGILKTVTLEDPRDIWYYNLKKSNNTFYINIATNAANNNTHSI
                     FFNQKLFDKNGKFIGVTGVGVPVETVKQVIDYYHNKYSKNIYLSDIEGRVVLYSSKTG
                     LLGAKIGSIPGLLGKEELILSKSPQSFMVENHGEIIHINSRYIPELNWILIVEQIENS
                     ITANIKISLYINIAVSILICIIILIIMLFLMSRYNKKMRQLATTDKLTDTYNRTAFDL
                     YIEIMRKESIRSKQPFALSILDLDFFKGINDTYGHSAGDQVLRKTTEIIKASIRESDI
                     LFRWGGEEFLILLRNCQLKEAAEIMDKIRKTINKAIFYFNNTPITITISAGVTVYQNT
                     ENIDVTINRADQLLYDAKRSGRNKTRY"
     gene            2296..>2533
                     /locus_tag="P5123_13420"
     CDS             2296..>2533
                     /locus_tag="P5123_13420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009646131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I tRNA ligase family protein"
                     /protein_id="HRX17308.1"
                     /translation="MKENLATVYNSSEIEKDALKSWSDSDSFAADPSKPGDPFAIVIP
                     PPNVTAALHLGHALNNTLQDILIRKNRMEGKNTLW"
BASE COUNT          797 a          446 c          495 g          795 t
ORIGIN      
        1 tcaagagaca gggggtctgg ttttggaaga agagacagct catcaaggtt ctcttcagat
       61 aaaagacgta caggtcctgg aagaagcaga aatgcccgta gtgagggctc agctaacagc
      121 agacctccct acgaaaaaaa acgaaaacct gacgagagct atagccgaat gtacatgaac
      181 gccggccgta aggacggaat tggtgttgtt gaccttatca gcttattaaa agaaaaaacc
      241 cgggtaagaa atatagaagt cggtaaagta gacataatgg acaagctgac tttttttgag
      301 gttctgaaaa aagatgaaaa acttgttgtt tcatccatga aaaataccgt cttaggctca
      361 agggctgtca gggtagaacc ggcggatgaa aacaagactg taactgcaaa aggcaaatct
      421 tctggcaaat caaaagttga acgaagctct aagcccaaag gtgcttcaaa taaaccggcg
      481 ggctcatcgc gaaaaaaaga ttaaaagaat cagattgatc attctatttt ataaaaataa
      541 aacccgggaa tttcagtacc gggtcttgtt tcgtcctgac cgtttggcat cataaagaag
      601 ctgatctgct ctgtttattg ttacgtcaat attttctgta ttctgataaa cagttactcc
      661 agcgcttatg gttattgtta taggggtatt attaaaatag aaaatggctt tgttaatggt
      721 ttttcgaatt ttatccatta tttcagcagc ctcttttaac tggcagtttc tcaaaagaat
      781 cagaaattct tcaccacccc atctgaaaag aatgtcactc tctcttatgg aagcttttat
      841 tatttcagtt gtttttctta aaacctggtc tcctgcagag tgaccgtatg tgtcattaat
      901 gcctttaaaa aaatcaagat ctaatattga aagagcaaat ggctgttttg aacgaatgct
      961 ctcttttctc atgatttcga tatacagatc aaatgctgtg cggttatagg tatctgttaa
     1021 cttgtctgta gtggccagct gtctcatttt tttgttgtaa cgggacatca gaaaaagcat
     1081 tataatcagg attataatac agataagaat actgaccgcg atatttatat ataaggaaat
     1141 ttttatattt gctgtgatgc tgttttctat ctgttcgaca ataagtatcc agttcagttc
     1201 cggaatatag cggctgttaa tatgaataat ttcgccgtga ttctcgacca taaatgattg
     1261 cggtgatttt gacagtatca gctcctcttt gcccagaagt ccgggaatac tgccgatttt
     1321 cgcgcccagc aagccggttt ttgaactgta aagcacgact ctgccttcaa tgtcggaaag
     1381 atagatgttt ttgctgtact tattatgata atagtcaata acctgtttaa cagtttcaac
     1441 cgggactccg acgccggtta cgccgatgaa tttaccgttt ttatcaaata atttctggtt
     1501 aaaaaatatt gaatgagtgt tgttgtttgc cgcatttgta gctatgttta tataaaaagt
     1561 attattgctt ttcttaagat tatagtacca gatatcacgt gggtcttcga gcgttactgt
     1621 ttttaatatt ccggcctgaa aatagtattt ctttgagttt tcagaaatta caaaagccga
     1681 gcttaatcct aaatatcgaa cgtactgttt cagatagttt tctacccggc taagttcttt
     1741 ttctcctgaa atcagccagt cttttaaaaa tatatcgttt gccattgctg atgatatgta
     1801 ttttgaagca agcagatcgc ttttgatctc ggaatagatc gttccggtgg taagcggtat
     1861 ctcgttgctt gtaatctgtg accgcagtga gtgcctggag acaaaaaagc tggataatgt
     1921 ggttaaaata aaaccgaaca tcagaattat tatcgttaaa gatatatata actttgttct
     1981 aatcacgcaa ttctccgtta agtaaaatcg aaatgacgca ttgttcttta ataaaccctg
     2041 atatatttaa aatttttaca aatataagaa acttttgaaa caatatcaac atacaattac
     2101 ctgggctttg aagagaacct tgaaaaaagc atgatatttt tcaagaatat aatcttgctg
     2161 tgttaattct ggtgggcttg gaggcatata cctgctaatc ctggccattt tttaaaaaat
     2221 taattgtcta aaatctaatc tatataagac tgttgtcaaa ataggtaact atacaaacta
     2281 accaggaaga tatatatgaa agagaatttg gctacagtct ataattcaag tgaaatagag
     2341 aaagatgcat tgaaaagctg gtcagactcg gacagttttg ccgctgatcc ttcaaaacca
     2401 ggtgatcctt ttgcaattgt gattcctccg ccgaacgtaa ccgcggcatt acatcttggg
     2461 catgctctta acaacacttt gcaggatatt ctgatacgta aaaaccgtat ggaaggtaaa
     2521 aacacactct gga
//
LOCUS       DAOTGZ010000316         2517 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_3064
            NODE_49104_length_2517_cov_5.450853, whole genome shotgun sequence.
ACCESSION   DAOTGZ010000316 DAOTGZ010000000
VERSION     DAOTGZ010000316.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868088
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 2517)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2517)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:22:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,785
            CDSs (total)                      :: 2,751
            Genes (coding)                    :: 2,732
            CDSs (with protein)               :: 2,732
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 12 of 19
            Pseudo Genes (internal stop)      :: 5 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2517
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_49104_length_2517_cov_5.450853"
                     /isolate="AMR_MDS_3064"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(183..1142)
                     /locus_tag="P5123_13425"
     CDS             complement(183..1142)
                     /locus_tag="P5123_13425"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRX17309.1"
                     /translation="MRVKTKKLLLTMVFITVATSSLCQKTVFASGKHTTIQLPRSKAI
                     INNNFDITQNHHFVLYDTDPTVSISVSIEHFPNNSGQSTLKKRISEIRKANKYFYQLE
                     NKKITIGNNSAQLVHLEYCRDGYSPCNSEQYVLIYTSTESVLICATSSPNDMENNMAI
                     WESMYNSFSFEHSKAYKLPYGSFSPPKSMRDQSFINCRLPEGRSFSINIYNREPYPKT
                     EMFSDSLLLRIYCVIRFWRPFNKSYGSKKKTYTINGRKAYYTESYKIDNFRTKYDVDA
                     QLYVPDDRYPLTITISSSKMSTFDYYRKDMDLLFKSVLESWDD"
     gene            complement(1224..2327)
                     /locus_tag="P5123_13430"
     CDS             complement(1224..2327)
                     /locus_tag="P5123_13430"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="patatin-like phospholipase family protein"
                     /protein_id="HRX17310.1"
                     /translation="MNTKERDSEKYPIIIRAGKRALEHIKKNGLNDDDIDTFAGASGG
                     PKGLMLAGLDQKIFTEWFTKREKALNMIGSSIGSFRLAAIAQKNPAEKIRIFYDRYIN
                     QQYSDTPDAAEVSNEARAILNYYIDKDSIEHILSHPFMRLHIIAARSRGLGKSDCKIT
                     LAPFFAAAYTMNMFDRKNLRFFFERYIFRDCRSAILENQFNDIITKTVNLNKQNFYDA
                     LMASGAIPFAMKRVTDIEDVHGVFRDGGLIDYHFDLPFNDISEKLVLYPHFFDKLCPG
                     WLDKKIKNRKPSPNNYSNVVMIAPNESFIESLPGSKIPDRNDFMLYKNCPEKRIEIWE
                     QVVKRSFELADAFMHLTRTGEIANAATPIEEIL"
BASE COUNT          670 a          451 c          489 g          907 t
ORIGIN      
        1 attattaatc ttgttttata aggttttatt gatttgattc caatacataa tttttgcttt
       61 tgaatgtccg ctaagttgac atcctgtcaa aagcgctaca ccgccgacat cgtgtcggct
      121 tcagtttacg ttatcttgaa gtgctggttt tatagatttt gttctaaacg gttttgtgca
      181 attcaatcgt cccatgattc aagcacgctt ttaaacaaca gatccatatc ttttcggtaa
      241 taatcaaaag tgctcatttt tgaagagcta atcgttatag tcaaagggta gcggtcatcg
      301 ggcacatata actgagcatc gacatcatat tttgttctaa aattatcgat tttatacgat
      361 tctgtataat aagcttttct accattgatt gtatacgttt tctttttgct tccataggat
      421 ttattgaatg gcctccagaa acgaattaca caataaattc ttaataataa tgaatctgag
      481 aacatttctg tctttggata tggctctcta ttgtagatgt ttattgaaaa gctgcgacct
      541 tcgggtaatc tgcagttaat aaagctttga tctctcatcg attttggcgg agaaaaactt
      601 ccataaggaa gtttgtatgc tttagaatgt tcgaaagaga acgagttgta cattgactcc
      661 caaatcgcca tgttgttttc catgtcatta ggacttgacg ttgcacaaat taatacagat
      721 tctgtacttg tatatattaa tacgtattgc tcactattac atggagaata gccatctctg
      781 caatattcca gatgaactag ttgagcggaa ttgtttccta tggtgatttt cttgttttct
      841 aattgataaa aatatttgtt tgctttcctg atttcagaga ttcttttttt taaagtactc
      901 tgcccgctat tattgggaaa atgctctatg gaaactgaaa ttgatactgt cgggtcggta
      961 tcatataaca caaaatgatg attttgggta atatcaaagt tattgtttat tatagctttt
     1021 gaccggggta gttgaattgt agtgtgttta cctgatgcaa acactgtttt ttgacaaagg
     1081 gaacttgttg caacagtaat aaacaccatt gttagtagaa gtttttttgt tttaactctc
     1141 atatatacag ccctacgtat tatcattaca taaatttatc gttttttatc gtcgtatatc
     1201 gcttcattgt ataatggaaa ttatcatagt atctcttcta tcggagttgc tgcattggct
     1261 atttcgccag ttctcgttag gtgcataaaa gcatccgcaa gttcgaagct gcgttttaca
     1321 acctgttccc atatttcgat tcgcttttca ggacaatttt tatatagcat aaaatcgttt
     1381 ctgtctggaa tcttgcttcc gggtaaagat tctataaaac tctcatttgg agctatcatt
     1441 acaacgttgc tgtagttgtt aggtgacggt tttctgtttt tgattttttt atctaaccag
     1501 ccggggcaca gtttgtcaaa aaaatgtggg tacagtacca gcttttcgct tatatcgtta
     1561 aagggtaaat caaagtgata atcgatcagg ccgccgtcac ggaagactcc gtgtacgtct
     1621 tctatatctg tgacacgttt catggcaaag ggaatagctc cgcttgccat cagagcatca
     1681 taaaaatttt gtttattcag atttaccgtt ttggtgataa tgtcgttaaa ctgattttcc
     1741 agaatggcag aacggcagtc ccgaaaaatg tatctttcga aaaaaaatct caggttcttt
     1801 ctgtcaaaca tgttcatcgt gtaagcggca gcgaaaaaag gtgcaagtgt aattttgcag
     1861 tcgcttttgc ccaatccacg gctccgggct gcgataatgt gtaatctcat aaaagggtgt
     1921 gacaaaatat gctcaataga gtctttatca atataataat tcagtatggc ccgagcctcg
     1981 tttgatactt cggctgcgtc gggggtgtct gagtattgct ggtttatata tctatcataa
     2041 aatattctga ttttttcagc tggatttttc tgagcgatgg cggccagcct gaagcttcca
     2101 atggatgagc ctatcatatt aagagctttc tctcttttgg taaaccattc cgtgaagatt
     2161 ttttgatcaa gtccggcgag catcaatccc tttgggccac cggatgctcc tgcgaacgtg
     2221 tcgatgtcgt catcgttcag gccgtttttc ttgatatgtt ccagagccct cttgcctgct
     2281 cttattataa tcggatattt ttctgagtct ctttctttgg tattcatagt aagttcacaa
     2341 atgtaatttt aaaagatttt atgttaaaac ggttctattg cacctggtgt atagcttcca
     2401 gtttcgcgtt tgtttccaaa caggtcggta tcaagttctg taatcgagtt cagatctttg
     2461 cctgatccga taagcgggga atctgatgat ggtgtatagt caagattctc gttaagt
//
LOCUS       DAOTGZ010000317         2507 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_3064
            NODE_49383_length_2507_cov_4.418842, whole genome shotgun sequence.
ACCESSION   DAOTGZ010000317 DAOTGZ010000000
VERSION     DAOTGZ010000317.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868088
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 2507)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2507)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:22:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,785
            CDSs (total)                      :: 2,751
            Genes (coding)                    :: 2,732
            CDSs (with protein)               :: 2,732
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 12 of 19
            Pseudo Genes (internal stop)      :: 5 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2507
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_49383_length_2507_cov_4.418842"
                     /isolate="AMR_MDS_3064"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..260
                     /locus_tag="P5123_13435"
     CDS             <1..260
                     /locus_tag="P5123_13435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011997461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="NGG1p interacting factor NIF3"
                     /protein_id="HRX17311.1"
                     /translation="NELFKTGAGKLSNYDCCAFETAGTGSFRPLEGSNPFIGKTGLIE
                     SVKETKIEMILEKKIMNKVRQCIIEHHPYETPAFHFTKIEL"
     gene            complement(265..>2507)
                     /locus_tag="P5123_13440"
     CDS             complement(265..>2507)
                     /locus_tag="P5123_13440"
                     /inference="COORDINATES: protein motif:HMM:NF018884.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016791 - phosphatase activity [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="SpoIIE family protein phosphatase"
                     /protein_id="HRX17312.1"
                     /translation="YSALSVLFNIFQMIIYGENLSSLHLSITDAHSLVEKVNQNSVLP
                     YIEILESFTSVLNNETIYSEEELTEMFKSHVYHKQPHNNPMFTLFTNLLLSVLFFFFG
                     YKKAAKRRITSMSEAFYSIKYSFIYNDFVFFKSLILAGSLELSDRAEYKKTCLYLEKA
                     IERYRSVICGGKGSSVVRLYLLQAELLRIRGKNLKAEFMYDRAIKNLDIEYNAMAGAI
                     ANEITASFFFSGGRHVIARAYIQDAFYNYQRWGAMSKVRKMERDFPEFINLHTASSLI
                     TKNGEVIANGSDEIDLGAVIKASHLITGEIYYETLITRLMNIIMEHSGAVRGCLILSE
                     GGALSIEAESRVSNSIDTALKSEIIRENTTSVPVSITNYVSRKRKAVILDDALTTPLF
                     SSDIYIISNACRSILCLPIVHRENFAGLLYLENNLAQGVFSSSRVRLLELVCAQAAIS
                     IQNSKYYNELKDYSQLLETRVQSRTRELEQVNENLMDINRLLRDARIEAERDMAMASN
                     VQLSFMPTDYKDKSSWDIAFSYKPMSAVSGDFYDFYEKDGVLHGAGVFDVSGHGISSG
                     LITVIARSIISRIFCENLNLSLKTVIQMINSMLIQEIGHTNNYLTGVLLRLKKNSIEY
                     VNAAHPNIIKYDCRKCRAFELRKEDETWRGGFLGVAAMKLPFGEMNVPMNKGDLVVLF
                     SDGVTEGGTNRSGEFGYEGIIKALEGGIMGSAHDVLDEINKRFFKISGDTGLRDDYTV
                     VVIRKK"
BASE COUNT          829 a          523 c          427 g          728 t
ORIGIN      
        1 aaaatgagtt atttaaaacg ggagcaggca agctgtcaaa ctatgactgt tgtgcctttg
       61 aaactgccgg aacaggctct ttcagacctc ttgaaggcag taatccattt ataggcaaaa
      121 ctggattgat tgagtctgta aaagagacaa aaatagaaat gattctggaa aagaaaataa
      181 tgaacaaagt gcgacaatgt ataatagagc accatccgta cgaaacaccc gcatttcact
      241 tcacaaaaat agagctttag ttggctactt ttttctgata acgactacag tataatcatc
      301 cctaagaccg gtatctccgc ttatcttaaa aaaacgcttg tttatttcat caagtacatc
      361 atgtgcactt cccattatac ccccttcaag tgcttttata attccctcat aaccaaattc
      421 accgcttcta ttggtgccac cctcagtcac accatcagaa aaaagcacta ctaaatctcc
      481 tttattcatg ggaacattca tctcaccgaa aggcagcttc atcgctgcta ccccaagaaa
      541 gccgccacgc caagtctcat cttccttacg taattcgaat gcccgacact ttcgacaatc
      601 atatttaata atatttggat gagccgcatt gacatattcg atactgtttt tctttaacct
      661 taacaataca ccggttaaat agttattggt atgtccaatt tcctgaatta acatgctatt
      721 tatcatctgt atgacggttt ttaacgataa attcagattt tcacagaaga tacgggaaat
      781 tatagaacga gctatgacgg ttatcaaacc ggacgatatc ccgtgacccg aaacgtcaaa
      841 cacacccgct ccatgaagca ctccatcttt ttcgtaaaaa tcataaaaat ctccagaaac
      901 agcgctcatg ggtttgtaac taaaagcgat atcccatgat gatttgtctt tataatcagt
      961 cggcataaag cttagctgaa catttgaggc catcgccata tctcgttcag cctcgattcg
     1021 ggcatcgcgt aaaagacggt taatatccat aagattttcg ttaacctgtt caagctccct
     1081 tgtccttgac tgaaccctgg tttcaagaag ctgggaataa tctttgagtt cattatagta
     1141 tttggaattc tgaattgaaa tagctgcctg agcacagaca agttcaagaa gccgtactct
     1201 cgatgacgag aaaaccccct gtgccaaatt attttccaaa tataagagcc cagcgaaatt
     1261 ctcacgatga actataggaa ggcaaagtat ggaacgacaa gcattagaaa tgatatagat
     1321 atctgatgag aaaagaggcg tagtaagtgc atcatcaaga ataacagcct tgcgtttacg
     1381 cgaaacgtaa ttagttattg aaaccggaac cgatgttgta ttttccctga taatttcact
     1441 ttttaaggct gtatcaatac tgtttgaaac ccgtgactcg gcttcaattg acaaggcccc
     1501 tccttccgat agaataagac aacctcttac agctccggag tgctccataa taatattcat
     1561 aagacgagtg atcaatgttt catagtaaat ctcacctgta ataagatgtg atgctttaat
     1621 aacagctcct aggtcaattt catctgaacc attagcaata acctctccat ttttcgtaat
     1681 caaagacgat gcagtatgaa gatttataaa ttctgggaaa tcacgttcca tttttcgaac
     1741 cttggacatt gccccccatc gctggtagtt atagaaagca tcctgtatat aagctctggc
     1801 tataacatga cggccaccag aaaagaaaaa agaagcagta atctcgtttg caattgcgcc
     1861 agccattgcg ttatactcta tatcaagatt ttttatcgca cggtcataca taaattctgc
     1921 tttaagattt ttcccacgaa ttctaagaag ttctgcttgc aaaagataga gccttactac
     1981 agacgaaccc ttaccaccac aaattaccga gcgatatctc tctattgctt tttcaaggta
     2041 aagacatgtt tttttatatt cggctctatc gctaagctct agcgaaccgg caagaatcaa
     2101 cgacttaaaa aaaacaaaat cgttgtaaat aaaactatac tttatgctgt aaaacgcttc
     2161 agacatcgaa gtaattctgc gttttgcggc ttttttgtaa ccgaagaaaa aaaacagaac
     2221 agaaagcaaa agatttgtaa acagcgtaaa cattggatta ttatgtggct gtttatggta
     2281 aacatgagat ttaaacattt cggtgagttc ttcttcacta taaatggtct cattatttaa
     2341 aacagaagta aaactctcaa gaatttctat atatggcaat acagagtttt ggtttacttt
     2401 ttcaacaaga gagtgagcat ctgttataga aagatgcaga gaagacagat tctctccata
     2461 aattatcatc tgaaaaatat taaaaagaac agaaagcgca ctatatt
//
LOCUS       DAOTGZ010000318         2487 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_3064
            NODE_49889_length_2487_cov_4.143503, whole genome shotgun sequence.
ACCESSION   DAOTGZ010000318 DAOTGZ010000000
VERSION     DAOTGZ010000318.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868088
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 2487)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2487)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:22:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,785
            CDSs (total)                      :: 2,751
            Genes (coding)                    :: 2,732
            CDSs (with protein)               :: 2,732
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 12 of 19
            Pseudo Genes (internal stop)      :: 5 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2487
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_49889_length_2487_cov_4.143503"
                     /isolate="AMR_MDS_3064"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            36..1142
                     /locus_tag="P5123_13445"
     CDS             36..1142
                     /locus_tag="P5123_13445"
                     /inference="COORDINATES: protein motif:HMM:NF013696.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M20/M25/M40 family metallo-hydrolase"
                     /protein_id="HRX17313.1"
                     /translation="MAINRERLIKTTIELIDIYSPSGKEIQIVDHIAEYLKKNGISYI
                     KQPVSDERCNLLINTDTASRDIVFMGHIDTVPAYNLDDYKSTIKDNMVIGLGACDMKG
                     ACAAMIEAFICYKEKYGKLPPISLALVVGEEESGDGAACFCDEYFYEWAVIGEPTSMY
                     PAFSHFGYLEMSITTTGTRKHASMVNFTKNAVHEMLAALNTITKFLDTECRGCVYNLR
                     DVSSSNAGFVVAERCEAWIDIHIPPFYTAQHITEKIKMMIENNFPDSDEFAYDFPTMH
                     TGYSLQKEGIVPEIFSSISDKPDNDSFPSDSDGAILWKAGIKPVIIGPGSLAVAHTQE
                     EFVCIDELVNAAETYFNFSDIYSKQLVDKKTCTD"
     gene            complement(1345..1851)
                     /locus_tag="P5123_13450"
     CDS             complement(1345..1851)
                     /locus_tag="P5123_13450"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRX17314.1"
                     /translation="MDKTDFRTSIDVEISRLERIEEYARKHNMDVSGVMNTVLDMYSK
                     KLVKNPVCNDTLVKYQERGTNYKSVHIRFSSISKRKLKDIANLHCYSLSYMLYLAMDF
                     FDEFMDGVKEFVRPCFHAAFVYHFFGFPYFINSWGMNFYEGQDHSSGQQSRKEPHLLC
                     WDHFFADY"
     gene            2157..>2487
                     /locus_tag="P5123_13455"
     CDS             2157..>2487
                     /locus_tag="P5123_13455"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRX17315.1"
                     /translation="MKKLISFTVNRPLPVLLVLLGITIILFTGIPKLRLEPSSEALMP
                     KDSPEYLYNMRMKQVFGDSRLFMLTLIEPDSKALFSKESFELLNELVEQIEEFRVFNL
                     DREQKRLG"
BASE COUNT          787 a          498 c          474 g          728 t
ORIGIN      
        1 tatgaaaacc aaaaatcatg catcaggaaa aagcggtggc gataaataga gaacggctga
       61 taaaaaccac aatcgaactg attgacatct acagcccttc cggaaaagaa atacagatag
      121 ttgaccatat tgctgaatat cttaaaaaaa acggcatcag ttacataaaa cagccagtct
      181 cggacgaacg gtgtaattta ctcataaata ccgatactgc ttcaagggat attgtgttta
      241 tggggcatat agacactgtt ccggcgtata accttgatga ctataaatcc acaatcaaag
      301 acaatatggt aatcgggctt ggcgcatgcg atatgaaagg cgcttgtgcc gcaatgatcg
      361 aagcttttat ctgctataaa gaaaagtatg gcaaattgcc cccaatatct ctggcattag
      421 ttgtgggtga agaggagtcc ggtgacggtg cagcgtgttt ttgtgacgaa tatttttatg
      481 aatgggcagt tataggcgaa ccgacatcca tgtatcccgc gttttcacat tttggttatc
      541 ttgaaatgag cattaccact accggaactc gcaaacatgc ttcgatggtt aatttcacca
      601 aaaatgctgt gcacgagatg ctggcggcac tcaacactat aaccaaattt cttgatacag
      661 aatgccgggg ctgtgtgtat aatctgcgag atgtcagcag cagcaacgcc ggttttgtag
      721 tagccgaacg atgcgaagcc tggatagaca ttcatatccc accgttttac actgcccagc
      781 acataacaga aaagattaaa atgatgatcg aaaacaattt tcctgactct gatgaatttg
      841 cttatgattt tcccacaatg cacactggat actctctgca aaaagaggga atcgtaccag
      901 aaatattttc gtctatcagt gacaaacctg ataacgattc ttttccaagc gattctgacg
      961 gtgccatact ctggaaagcc ggaatcaaac ctgtaataat cggaccgggc tctcttgctg
     1021 tagcacatac acaggaagag tttgtttgca tagacgagct tgtaaacgct gctgaaacct
     1081 atttcaattt ttctgatatc tattcaaaac agctagtgga taaaaaaacc tgcactgatt
     1141 aggatgctat tatatacggt ttcagctctt attctatatt agtttactgc gtttataaaa
     1201 aagacagaag ggtctccaaa tcattataaa gctgtataaa gttaattttg ttcgtgcgga
     1261 taaaacctga ttgttctgct cataagcggc ctccggtttt gtttcagttg attctttttt
     1321 ttcgtgtttc gttttttatt tttctcaata atcggcaaaa aaatgatccc aacacaacag
     1381 atgcggctct tttcgggatt gttgtccaga agaatgatct tgcccctcgt aaaagttcat
     1441 accccagcta ttaatgaaat atgggaaccc gaaaaaatgg taaacaaaag cagcatggaa
     1501 acagggccgc acaaactcct taactccatc cataaactca tcaaagaagt ccattgccaa
     1561 ataaagcatg taagacaaac tgtaacagtg aagattagcg atatctttca gtttcctttt
     1621 cgagatactt gaaaaccgta tatgcacaga cttataatta gtgcctcgtt cctgatattt
     1681 aacaagagta tcattgcata caggattttt aaccagtttt ttagaataca tatcaagaac
     1741 agtgttcatt acgcccgaaa catccatatt atgcttcctg gcatactctt caattctttc
     1801 cagtctgctt atttcaacat caattgatgt tctaaaatca gttttatcca taacatacct
     1861 ccaaatgctc tatatatgta aacttaattg gaggctgttt tgttaaaatt ttttgataaa
     1921 ttttcataat tcctgtatgc gcaggaatta cggtatagcg attatttcag caatcacata
     1981 aatagcgtga tagaaagtcc cactattaag tatttacatg ttttttagtt tcagaattat
     2041 aaatcagaaa cataactcag ttacagatat ataaatccga aagaccgggg catacattat
     2101 agtgtattct tttgtttaac agggcaacac agccctatca caacaggaga atacatatga
     2161 aaaaacttat cagctttaca gtaaacagac ctttgcctgt tttgcttgtt cttttaggca
     2221 tcacaattat cttgtttacg ggtattccaa agctgcggct ggagccatcg tccgaagcat
     2281 taatgccaaa agattcaccg gaatatctct ataacatgcg catgaagcag gtgtttggtg
     2341 acagcaggct tttcatgctt acacttatcg agcccgacag taaagccctg ttctcaaaag
     2401 agagtttcga actgctgaac gagctagtcg aacagattga agagttccgg gtctttaacc
     2461 ttgaccgtga acagaaaaga ctgggag
//
LOCUS       DAOTGZ010000319         2481 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_3064
            NODE_50045_length_2481_cov_5.685491, whole genome shotgun sequence.
ACCESSION   DAOTGZ010000319 DAOTGZ010000000
VERSION     DAOTGZ010000319.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868088
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 2481)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2481)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:22:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,785
            CDSs (total)                      :: 2,751
            Genes (coding)                    :: 2,732
            CDSs (with protein)               :: 2,732
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 12 of 19
            Pseudo Genes (internal stop)      :: 5 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2481
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_50045_length_2481_cov_5.685491"
                     /isolate="AMR_MDS_3064"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1038
                     /locus_tag="P5123_13460"
     CDS             <1..1038
                     /locus_tag="P5123_13460"
                     /inference="COORDINATES: protein motif:HMM:TIGR03506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook protein FlgE"
                     /protein_id="HRX17316.1"
                     /translation="YTRAGTFSTDRDGTLVNPSNGLKVQGWMATTDADNNPVLNTQGT
                     PDDLVIPIYQKLNAKETSFVRYQCNLDSKLPIIPDSMPAAQRASAAVVTNIDTYDNLG
                     DPHRVTLTLWKTATNEWTASAAMTDAEGPVTLDVNALTGENLPNPNSRMRLTFSPDGK
                     LVSASDDATSDSVNSGNLSANVNYRIAGDPAVRTLRLNLGTSGMINGITQFSSPSTTK
                     GVEQDGYSMGYMEAFNIDNSGVITGVYSNGIQRPIGQVAMSVFTNPQGLTAVGENLFE
                     MSNNSGLANIGEANQGGRGKIVAGTLEMSNVDLSDQFTDMIITQRGFQANSRTITTSD
                     QMLQEVINLKR"
     gene            1415..1762
                     /locus_tag="P5123_13465"
     CDS             1415..1762
                     /locus_tag="P5123_13465"
                     /inference="COORDINATES: protein motif:HMM:NF024857.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043856 - anti-sigma factor antagonist
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="STAS domain-containing protein"
                     /protein_id="HRX17317.1"
                     /translation="MLEIDYVSFSNAPAMIVRGRYDISSITVFEDSFKELLERAGTAV
                     GVNLKDLTYIDSSGIGSLIRCFNLARKRGITCYFYDINSDIMTVFQLSRLDSYLKVVT
                     NEEFELRFSDSSI"
     gene            1790..>2481
                     /gene="trpS"
                     /locus_tag="P5123_13470"
     CDS             1790..>2481
                     /gene="trpS"
                     /locus_tag="P5123_13470"
                     /EC_number="6.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007295080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004830 - tryptophan-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006436 - tryptophanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan--tRNA ligase"
                     /protein_id="HRX17318.1"
                     /translation="MTRILSGIQPSGSLHIGNYLAMMKPMIALQDDNELYCFIVNYHA
                     LTSLSDPEKLKKNTMDAAIDFLALGLDPEKANFWIQSDLPEVQELTWILSCNTSLGLL
                     ERSHSYKDKIAKGLTPNAGLYTYPILMAADILLFQAEKVPVGKDQKQHLEITRDIASS
                     FNANFGETFVLPEPVIEDNLAVIPGIDGQKMSKSYDNTICMFEEEKKMRKRIMKIVTD
                     SKGVDEPKDPDK"
BASE COUNT          787 a          480 c          458 g          756 t
ORIGIN      
        1 tacactcgtg ccggtacttt tagtacagat cgagacggaa cacttgttaa cccttcaaac
       61 ggccttaagg ttcaaggctg gatggctacc acagacgcgg ataataatcc ggtacttaac
      121 acacaaggta ccccggatga cctcgttata cctatttatc agaaacttaa cgcgaaagag
      181 acatcttttg taagatatca gtgtaaccta gattcaaaac ttccgattat tcctgattca
      241 atgcctgctg ctcaaagggc atctgctgct gtagtaacca acattgatac gtatgataat
      301 cttggagatc cacaccgggt caccctaacc ctttggaaaa cagcaacaaa cgaatggacc
      361 gcttctgcag ccatgactga cgctgaaggt cccgttacac tggatgttaa tgcgttaaca
      421 ggtgaaaacc ttccaaatcc gaatagtcgc atgcgtttaa ctttttcacc tgatggaaaa
      481 cttgtttcgg catccgatga tgcaacttca gattcggtaa acagtggaaa tctttctgca
      541 aatgttaatt accgtattgc cggagatcca gcggtacgaa ctcttcgtct taatttgggt
      601 acatcaggca tgataaacgg aattacacag ttttcttctc catctacaac caaaggtgtt
      661 gaacaagacg gatactcaat gggatatatg gaagcattca atatagataa ttcgggtgtt
      721 atcactggag tctattcaaa cggtatccag cgccctatag gtcaggttgc aatgtccgtt
      781 tttacaaacc ctcaagggtt aacagcagtg ggtgaaaacc ttttcgaaat gtcaaacaac
      841 tcaggattgg caaatattgg cgaagccaat caaggtggac gcggaaaaat tgtagctggt
      901 actcttgaaa tgtcaaatgt tgatctttct gatcagttta cagacatgat aattacccaa
      961 cgcggttttc aagcgaactc acgcacaatt acaacatcag accagatgct tcaggaagtt
     1021 ataaacctga aaagataaca gagtgagtat taaaacctat taatgttata aattcactta
     1081 cggcaacaac tgtcttcatt tagaataatc tttcataaaa agagaagagt tagccctctt
     1141 ctctttttgt ataacaaaaa taattgaacc atactcacat attttcaacg ttatgtttcg
     1201 taacaaaaat ttatgtttga cacattttaa atttcatgat tatagataat tagaaaatgt
     1261 tcagattaga tatttttttg ataactatac aaaatattct tgtttgttta aattcagttt
     1321 caaatttggc cttttatttt aaaaatattt tcagtcttac cttgtatatt tgttcaaata
     1381 tgaatctacg gattatacaa aattttggtg tacaatgctt gaaatagatt atgtatcttt
     1441 ctcaaacgca ccggctatga ttgtacgtgg ccgatatgat attagttcga taacggtttt
     1501 cgaagattcc tttaaggagc tccttgaacg cgctggaaca gcggttgggg ttaatcttaa
     1561 agatcttacc tatatcgatt catctggtat aggttcgctg atacgctgtt ttaatcttgc
     1621 cagaaaacgg ggtatcacat gttattttta tgacataaac agcgatatta tgactgtttt
     1681 tcagctatca agacttgaca gttatctaaa agttgtaaca aacgaagaat ttgaattaag
     1741 attcagcgat tcaagcatat aaaactgatc attttacagg agttttttta tgacacgcat
     1801 tctatccggc attcaaccct ctggttctct tcatataggc aattatcttg ccatgatgaa
     1861 accaatgatt gctctgcagg atgataatga actgtactgc tttattgtta attatcacgc
     1921 cttgacatca cttagtgacc ccgaaaaact aaaaaaaaat acgatggatg cagctattga
     1981 ttttcttgct ctgggacttg acccagaaaa agcaaatttc tggattcaat cagatttacc
     2041 tgaggttcaa gaattgacat ggattctttc ttgcaacact tcgcttggtc tgcttgaacg
     2101 aagtcactct tataaagaca agatagctaa aggtctaact cctaacgcag gcctctacac
     2161 atatccgatt cttatggctg ctgatattct tcttttccag gctgaaaagg ttcctgttgg
     2221 caaagaccag aaacagcatc ttgaaataac tcgtgatatc gcatccagct ttaatgcaaa
     2281 tttcggagaa acctttgtct tgcccgagcc cgttattgaa gacaatcttg ccgtaatacc
     2341 cggaattgac ggacaaaaga tgtctaaaag ttacgataat actatctgta tgtttgaaga
     2401 agaaaaaaag atgagaaaac gaattatgaa gatagtcact gattcaaaag gtgtcgatga
     2461 acctaaagat cctgataaat g
//
LOCUS       DAOTGZ010000320         2478 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_3064
            NODE_50145_length_2478_cov_4.244325, whole genome shotgun sequence.
ACCESSION   DAOTGZ010000320 DAOTGZ010000000
VERSION     DAOTGZ010000320.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868088
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 2478)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2478)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:22:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,785
            CDSs (total)                      :: 2,751
            Genes (coding)                    :: 2,732
            CDSs (with protein)               :: 2,732
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 12 of 19
            Pseudo Genes (internal stop)      :: 5 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2478
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_50145_length_2478_cov_4.244325"
                     /isolate="AMR_MDS_3064"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1116
                     /locus_tag="P5123_13475"
     CDS             <1..1116
                     /locus_tag="P5123_13475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013707085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-grasp domain-containing protein"
                     /protein_id="HRX17319.1"
                     /translation="AAKIGYPLMVRPSYVLGGRGMKIVQDEQMLRQYVAAAVGEVSPD
                     RPLLIDMFLEDAIEAEADAISDGEDAFVPAVMQHIEYAGIHSGDSACVIPPVAITDEQ
                     IQTIEEYTRRIAIEMGVIGLMNIQYAICKGVVYILEANPRASRTVPLVSKICNIPMAK
                     IATGIMLGKKLPDFNLSKKNISHFGVKEAVFPFNMFPDVDPVLGPEMRSTGEVLGLAD
                     SFGMAFYKSQEATKSILPTKGTVLLSISGDDKEKVLQPVKDFIALGFKITATDGTAEY
                     LESKGIKTEIIKKVHQGRPNIVDAITNGEIDLVVNTPVGKQGQFDDSYIRKSSIKHNI
                     PYITTIAAASAAVEGIKERCAQNYTVKSLQEYHKGIV"
     gene            1184..1846
                     /locus_tag="P5123_13480"
     CDS             1184..1846
                     /locus_tag="P5123_13480"
                     /inference="COORDINATES: protein motif:HMM:NF024811.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IA family hydrolase"
                     /protein_id="HRX17320.1"
                     /translation="MTDAIIFDMDGVLIDSKPLHYETDIAILKQIGLEKESSYLDRFV
                     GMTNPEMWQQIADENKLDINIEDILNTQIMLKLELLWKQNFSAIDGVVDLIRKIYSNN
                     TVIGVASSSSEIFIENVLKKIDIGKYITSYVSGENVPKGKPAPDIYIHIADVLGVKPA
                     KCIAIEDSYHGVVAAKRAGMKVVGYKNVNSGNQDLSLADMVIDDFVKLALRYNDGFEM
                     FD"
     gene            1857..2378
                     /locus_tag="P5123_13485"
     CDS             1857..2378
                     /locus_tag="P5123_13485"
                     /inference="COORDINATES: protein motif:HMM:NF025675.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YcxB family protein"
                     /protein_id="HRX17321.1"
                     /translation="MQIEYEVTKDDIIHFNKYHLMHSKETKAILIRSRLLQIFYVMVI
                     TIVLAYSTDNWIVFSVFGFIIVVLYMLRSRKNLIKNIEKNVSKSLNSDDNLVILGKQI
                     LDIHEDGLFSKGQASEGKTFWNGLTKIIENDGYIYIYLGTTKAFVIRKESITKGDPDI
                     FITELKKKMEPET"
BASE COUNT          784 a          414 c          551 g          729 t
ORIGIN      
        1 gcagcgaaaa tagggtatcc gctaatggtt cgcccgtctt atgttcttgg tgggcgcgga
       61 atgaaaatcg tgcaggacga acagatgcta cgccaatatg ttgcagcagc ggtgggcgag
      121 gtttctcccg atcgaccttt gcttattgac atgtttcttg aagatgctat cgaagccgag
      181 gcggatgcaa tctcggatgg agaagacgca tttgttcctg ccgtaatgca acatattgaa
      241 tacgctggaa ttcactctgg ggattctgcc tgtgttattc ctccggttgc cataaccgac
      301 gaacagatac aaacaatcga agagtacaca cgccgtatag ctattgaaat gggcgttata
      361 gggcttatga atatccagta tgctatctgt aaaggggttg tttatattct tgaggctaat
      421 cctcgcgcgt cgcgtactgt tcctcttgtt tctaaaatat gcaatatccc aatggccaag
      481 attgccacag gtattatgct gggtaaaaaa cttccggatt ttaatcttag caaaaaaaat
      541 atcagtcact ttggtgtaaa agaggcagtg tttcctttta atatgtttcc cgatgtggat
      601 ccggtactcg gtcccgagat gcgctcgacc ggagaggtgc ttgggcttgc ggattcattc
      661 ggtatggcct tttacaaatc gcaggaagct accaagtcca tactgcctac aaaggggacg
      721 gtgcttttgt cgatatcagg tgatgataaa gagaaggtgc tccagccggt taaagatttc
      781 attgcactcg gattcaagat cacagccaca gacggtacgg ccgaatatct tgaaagcaaa
      841 ggtattaaaa cagagataat caaaaaagtt catcagggtc gtcccaatat tgtagatgcg
      901 ataactaacg gagagataga tcttgtggta aacacacctg ttggtaaaca gggacaattt
      961 gatgactctt atatacgcaa gagctctatc aaacataata ttccgtatat cacaacaatc
     1021 gctgccgcat ctgccgcagt agaaggcata aaagagagat gcgcgcagaa ctatactgtt
     1081 aagtcactgc aggagtatca taaagggatt gtgtagtttg atttgtcttt tatcagaaat
     1141 tgacctgctt gagccagaaa acagatccta aatgggaaac aaaatgacgg atgctattat
     1201 ctttgatatg gacggtgtgt tgattgacag caagccgttg cattatgaga ccgacattgc
     1261 tattttgaag caaattggtc ttgaaaaaga gagctcctat cttgacagat ttgttggtat
     1321 gactaacccg gagatgtggc aacagatcgc ggatgagaat aaacttgata ttaatataga
     1381 agatatttta aatactcaaa tcatgttaaa acttgaattg ctctggaaac aaaatttcag
     1441 tgcgatagat ggcgttgttg atttaataag aaaaatttac tcaaacaata cagttattgg
     1501 tgttgcttct tcttcatcgg aaatttttat tgaaaacgtg ttaaagaaaa tagatatcgg
     1561 caagtacata acaagctatg tttctggaga gaatgtgcca aaaggcaagc ccgcgcctga
     1621 tatctacatt catatcgcgg atgtccttgg cgtgaaacct gcaaaatgta tagcgattga
     1681 agactcgtac catggtgtcg ttgcagccaa aagagctggt atgaaagttg tagggtataa
     1741 aaatgtaaat tcgggaaatc aggatctatc tttggccgat atggtaattg atgattttgt
     1801 aaaattggca ttaaggtata acgacggatt tgaaatgttt gattgaggta ttgagtatgc
     1861 aaattgagta tgaagttaca aaagatgaca ttattcactt taataaatat catctgatgc
     1921 attccaaaga aacaaaggca atattaataa gatcacgtct attacagatt ttttatgtaa
     1981 tggttatcac tattgtgttg gcatacagta ctgataactg gattgtgttt tctgtatttg
     2041 gttttataat tgttgtgctt tatatgttga ggtctagaaa aaacttaata aaaaatatcg
     2101 aaaaaaatgt tagcaagtct ttaaattcag atgacaatct ggtgattctt ggaaaacaga
     2161 tcctggatat acatgaagac ggtcttttct caaaagggca ggcttcagag ggtaagacgt
     2221 tctggaatgg tttaacaaag attatcgaaa atgatggtta tatttacatt taccttggaa
     2281 caactaaagc atttgtaatt agaaaagaga gtatcacaaa aggtgatccg gatattttta
     2341 ttacggagct taaaaagaaa atggaaccgg aaacataact aaggaatcag actccgtcaa
     2401 tctcaattgc tgatataaaa cctataacat cttcgtgtct taaaaatctg atttctatat
     2461 tttggtatat tatgctgg
//
LOCUS       DAOTGZ010000321         2460 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_3064
            NODE_50620_length_2460_cov_5.354262, whole genome shotgun sequence.
ACCESSION   DAOTGZ010000321 DAOTGZ010000000
VERSION     DAOTGZ010000321.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868088
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 2460)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2460)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:22:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,785
            CDSs (total)                      :: 2,751
            Genes (coding)                    :: 2,732
            CDSs (with protein)               :: 2,732
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 12 of 19
            Pseudo Genes (internal stop)      :: 5 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2460
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_50620_length_2460_cov_5.354262"
                     /isolate="AMR_MDS_3064"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..711)
                     /locus_tag="P5123_13490"
     CDS             complement(<1..711)
                     /locus_tag="P5123_13490"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRX17322.1"
                     /translation="MAFTVKDYRKKTGFCIRFVLIALLVLLGGSGHSVYATKSSASIK
                     AGEQKKAEKGLKDNKKFLYYINASVSNTGTAEDKELYSLAVKHDIVARFYYLRFSFSE
                     SFAQIRTAQEILIKLYRSNITAEVDRAMLYLNRIAPEAVYSRDPRARLYLRLAYRNAK
                     HAGIYKNMADHYKLTLYSMRLYKYVEAMKKAKEAKRYAVSAMLQIEMEDKHLLEREYH
                     SYDDIVKLIEKNSRSDIKE"
     gene            927..1847
                     /locus_tag="P5123_13495"
     CDS             927..1847
                     /locus_tag="P5123_13495"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRX17323.1"
                     /translation="MIKKPINSILEHTTSVANGRFTIFVVIIAALIIPSHPILKTVLP
                     WILGCVMFVNFITIKIKITNPLNRTGIAYFVVVAAIIPCAIALSNPGLPAAVQNGLIL
                     AIISPTALSSPVIASFTGADSSRLVTHILIAHIAAPVIYTIHLYYSLNLSDFTIPYFM
                     IILKVVTMILAPLIAACLYKKTLKNIAIPRLVLMKKILFYLLVFTAVSSASEKIKNLE
                     PLFALQLFITVFLLAVLLYFCGYITCYRQNKGITGALMFGHKNTGLSLWLAITYLPGE
                     SSAPLVLYIISHHIINGILISFNNKNPNLN"
     gene            complement(1945..2145)
                     /locus_tag="P5123_13500"
     CDS             complement(1945..2145)
                     /locus_tag="P5123_13500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020006769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold shock domain-containing protein"
                     /protein_id="HRX17324.1"
                     /translation="MEKGVVKWFNEQKGFGFITKEGGDDIFVHHTGIVGEGFRTLNDG
                     DNVEFDVEETEKGQRAVSVSRI"
BASE COUNT          721 a          529 c          385 g          825 t
ORIGIN      
        1 ctctttaata tcagaacggc tattcttttc tatcagtttt actatatcat catagctgtg
       61 atactctctt tcaagcagat gtttatcttc catttcgatt tgcagcatcg cgcttacagc
      121 ataacgtttg gcctctttgg cttttttcat ggcctcgaca tatttataca gcctcatgga
      181 atagagggtc agcttgtaat gatcggccat gtttttgtat atcccggcat gtttagcgtt
      241 ccggtatgcc agacgcaaat agaggcgggc acgaggatca cgcgaatata cagcctccgg
      301 agctattctg tttaaataaa gcatggctct gtcgacttcg gcagtaatat ttgatctgta
      361 gagcttaatc agaatttcct gagccgtacg tatttgcgcg aacgattcag aaaacgaaaa
      421 ccttaaataa taaaaacgcg ccactatatc atgtttaaca gcaagggagt ataactcttt
      481 atcctccgca gttccggtat ttgatacaga cgcgtttata taatataaga attttttatt
      541 atctttcagg cctttttcgg cttttttctg ttctccggcc ttaatgctcg cgctcgattt
      601 tgtggcataa acagaatgtc ctgatccacc caacagcacc agtagcgcga tcaagacaaa
      661 cctgatacaa aaaccggttt tttttctata atctttgaca gtaaaagcca tctatccatc
      721 cattaagaca attcatctct tttcacgcca gcttttatac tacaaaaacg cgtcacttct
      781 caactttatt atcgttgtta cttttcttca attgtacgta aaacagttgc ccaaaattga
      841 tatgcaggtc cagactatcc ctgaatcaaa ttttaaccgt taacaataca attatcgacc
      901 ccaaacgagg caaaagacag cttgcaatga ttaaaaaacc cataaacagt atattagagc
      961 atacaacttc agttgctaac ggccgtttta ccatttttgt tgtaataatc gcagccctta
     1021 ttataccttc tcacccgata ctgaagactg ttttaccatg gattcttggc tgtgtaatgt
     1081 tcgtgaattt tatcacaata aaaataaaaa tcacaaaccc tcttaacaga actggaatcg
     1141 cctatttcgt cgtggttgct gcaattattc cctgtgctat tgcgctatcc aatccgggac
     1201 ttccggcagc agttcaaaac gggctgatac tcgcaataat ctctccaaca gcgctaagtt
     1261 cacctgttat cgcgtctttt acaggagccg atagcagccg acttgttaca catatactta
     1321 ttgctcatat tgctgcaccg gttatttaca ccatacacct ctattacagt cttaatcttt
     1381 cagatttcac cattccctat tttatgataa tattgaaagt agttaccatg attctggcgc
     1441 ctctcatagc agcctgttta tataaaaaaa cactgaaaaa tatcgctatc cccaggcttg
     1501 ttcttatgaa aaagatactc ttttatctac tggttttcac tgcagtgtca agcgcttcag
     1561 aaaagattaa aaatctggaa cccctttttg ccttacaact ttttattacc gtatttctac
     1621 ttgctgttct gctctatttt tgcggatata ttacatgtta cagacaaaac aaaggtataa
     1681 cgggagccct tatgttcggg cacaagaaca caggattgtc actctggctt gcaataacat
     1741 atcttccggg agagagctct gctcctctag tactatatat tatttctcac catattatca
     1801 atggtattct tatttctttt aacaacaaaa accccaatct gaattgattc cggtttacta
     1861 aaaatcagat tttacataaa aaaaccgccc atcgggcgga attttttgta gtttttgata
     1921 atttgcgtaa tttcttatga cttgttatat acgagacaca gaaacggcac gttgaccttt
     1981 ctcggtttcc tctacatcaa actctacatt atcaccgtcg ttaagagttc tgaaaccctc
     2041 accaacgatg cctgtgtggt gaacaaagat atcgtctcca ccttcttttg tgatgaatcc
     2101 gaagcctttc tgctcattaa accactttac aacacctttt tccatacgtc ttaatactcc
     2161 aaattttatt aagccatgtt taaaaaaaca aagctttatc ttagtgaatt tatttaagct
     2221 gttttgtcaa gtttattaat cattaaatgc ccgtttgtta tcttaaacaa aagttaatat
     2281 tttttctcga attcgaatgt ttgacttaat cgggataagg caggtattta ttgactttaa
     2341 taaataaagt tataaaactg ccaataagtt ctataatttg gttaaacata tatataactg
     2401 ctattttttg gtgtaattat caaaaatatg cgaaataatt ctgaagatct gacaatattg
//
LOCUS       DAOTGZ010000322         2444 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_3064
            NODE_51068_length_2444_cov_5.167434, whole genome shotgun sequence.
ACCESSION   DAOTGZ010000322 DAOTGZ010000000
VERSION     DAOTGZ010000322.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868088
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 2444)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2444)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:22:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,785
            CDSs (total)                      :: 2,751
            Genes (coding)                    :: 2,732
            CDSs (with protein)               :: 2,732
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 12 of 19
            Pseudo Genes (internal stop)      :: 5 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2444
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_51068_length_2444_cov_5.167434"
                     /isolate="AMR_MDS_3064"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            65..1438
                     /gene="rimK"
                     /locus_tag="P5123_13505"
     CDS             65..1438
                     /gene="rimK"
                     /locus_tag="P5123_13505"
                     /EC_number="6.3.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012155337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S6--L-glutamate ligase"
                     /protein_id="HRX17325.1"
                     /translation="MKKNIIGCEEWINLPELSIPAIKARVDSGAKTSSLHAVNIKPFN
                     FENNIWVSFEVHPIQNNRKVVVHCKAPVVDKRNIRSSSGHSEKRYVVKTSTRIGQDQW
                     EIELTLTNRDSMGYRMLLGREAMKGRLLVDPEKRFCQGALDEANLAELYSNSRVQVSG
                     LKIALLASNPNLYSNRRIMEAGQQRGHEMIFLDIKKCYMKLEAMLPEVHYHGGEVLGD
                     IDAVIPRIRPAITFYGCALVRQFESLGAYCLNSAEAIAQSRDKLRSLQLMLSQGIDIP
                     LSGFANSPDNTEDLITMVGGAPLIVKLLEGTQGKGVVLAETRKAAESVINAFKSLKAN
                     ILVQEFIKEAQGRDIRCFVVDNKVIAAYQRISAPGEFRANMHLGGSAAKIKITPMERK
                     LAIRAAKVTGLKVSGVDIIRSVDGPRVLEVNSSPGLEGIEGITGMDIAGEMIRFIEKQ
                     IGWKSSI"
     gene            1508..>2444
                     /locus_tag="P5123_13510"
     CDS             1508..>2444
                     /locus_tag="P5123_13510"
                     /inference="COORDINATES: protein motif:HMM:NF012692.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family transcriptional regulator"
                     /protein_id="HRX17326.1"
                     /translation="MLKRDKKSNISKVLHALWIHKNASRVQVSRAIGLDKSTVTNIIN
                     EMIDIGLISVVAEGDSGPQGGRKPVLLKINPGFGYVVGAEIQPELFRMVVSDLDGTIS
                     DYGEYVFDSLADFNELFVKGICPFITNQQFSGKKILGLGIGLPGVVDPYRSMLMQSIP
                     LGISETMQLPGFDEFAEIMGGFTAPIIYENDANCCAWGELALGRSDGIRNFITILIEF
                     TDVSHPSYKTHAIALGVGIVIDGRVYHGKNYSSGEFRSLFWNEGNRTQFSLSGDDVDL
                     IKTDVDVQRRFLEELASHVAFLVNMFNLSYVFFGGD"
BASE COUNT          698 a          400 c          608 g          738 t
ORIGIN      
        1 cacaaaggct ttttgtaggc gatgaaaatt tttggttatt tcttatttta tggatggtta
       61 tttgatgaaa aaaaatataa tcggatgtga agagtggatt aatctccctg aactttcaat
      121 tccggctatt aaggctcgtg ttgattctgg agcaaaaaca tcttctttgc atgcggttaa
      181 tattaagcct ttcaattttg aaaataatat atgggtaagt tttgaggtac atccaattca
      241 gaacaaccgc aaggttgttg ttcattgcaa ggcgcctgtt gttgataaac gaaatatacg
      301 cagctccagt ggacattccg aaaaacgtta tgtagtcaag acgtcaaccc gtattgggca
      361 agatcagtgg gaaatagaac ttacattaac caatcgggac tcgatgggtt atcgtatgct
      421 tttgggacga gaagcaatga agggcaggtt acttgttgat cctgaaaaaa gattctgtca
      481 gggtgcgctg gacgaggcaa atcttgctga gctttacagt aattcaagag ttcaggttag
      541 cgggcttaaa atagctcttt tggccagtaa tcctaaccta tattcaaaca ggcgtataat
      601 ggaagctggt cagcagcgcg gacatgaaat gatctttctg gatataaaga agtgttacat
      661 gaaacttgaa gccatgctgc ctgaggttca ttatcatggc ggtgaggtgc ttggggatat
      721 cgatgcagtt atacctagaa tacgtccagc aataactttt tatggatgtg ccttggttcg
      781 tcagttcgaa tcattggggg cttactgctt aaactctgct gaggcaatag cccagtcccg
      841 tgataaactt agatctcttc agctgatgtt atcgcaagga attgatatcc cgcttagcgg
      901 attcgccaat tctcctgaca atacagaaga tcttattacc atggtcgggg gagctcctct
      961 aattgttaaa cttcttgaag gtacacaggg aaagggtgtg gttttggccg agactcgaaa
     1021 ggcagcagag agtgtaataa atgcctttaa aagtttaaaa gctaatatac tggtgcagga
     1081 atttattaaa gaggcacagg gtagggatat tcgctgtttt gttgtagata acaaggtgat
     1141 tgctgcatat cagcgaattt cggcaccagg tgaatttaga gcgaacatgc atcttggagg
     1201 aagtgccgct aagataaaga taactccaat ggagcgaaaa ttggctatac gagcggccaa
     1261 ggtcaccggt ttaaaggtgt ccggtgttga tattattcgt tcggtagatg gaccacgagt
     1321 tttagaggtt aattcgtcac cgggtcttga aggtatcgag ggtattactg gaatggatat
     1381 tgctggtgag atgataaggt ttatcgaaaa acagataggc tggaaatcat caatttaaaa
     1441 aaaagtttga cagttaagga acatctgaga tggttagttg aaattaaaac caactaacgg
     1501 aagttgaatg ttaaagcggg ataaaaagag caatatttcc aaggttttgc atgcactctg
     1561 gatccataaa aacgcaagcc gtgttcaggt gtcccgggcg attggccttg ataaatcgac
     1621 tgttaccaat attataaacg aaatgattga tattggatta ataagtgtag tcgcggaagg
     1681 tgattcagga ccgcagggtg ggcgcaagcc tgtgttgctg aaaattaatc ctggttttgg
     1741 ttatgtagtt ggtgccgaaa ttcagccgga acttttccga atggtggttt ccgatctgga
     1801 tggtacgatt tctgattatg gtgaatacgt atttgattca cttgctgact ttaatgagct
     1861 ctttgtaaaa ggtatatgcc cctttataac gaatcagcag ttttctggta aaaagatact
     1921 tgggctgggg attggtcttc ctggagttgt tgatccctac cgatctatgc tgatgcaatc
     1981 cataccactt ggaatatccg aaactatgca attaccaggt tttgatgagt tcgcggaaat
     2041 aatgggtggt tttacagcac ctatcattta tgaaaatgac gcaaattgct gtgcatgggg
     2101 agagctggct cttggtcgtt ctgatggaat aagaaatttt attacaatat tgatagagtt
     2161 taccgatgtg tctcatccaa gttacaagac acatgcgatt gcacttggcg ttggtattgt
     2221 aattgatggc cgagtttatc atggaaagaa ttattcctca ggagagttta gaagcctatt
     2281 ttggaatgaa ggaaaccgta cgcagttttc attatctgga gatgatgtcg acttaataaa
     2341 aactgatgtt gatgtgcagc ggcgttttct tgaagagctg gctagccacg tagcgtttct
     2401 tgtgaatatg tttaacctct cttatgtttt ttttggaggg gatg
//
LOCUS       DAOTGZ010000323         2423 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_3064
            NODE_51699_length_2423_cov_5.317145, whole genome shotgun sequence.
ACCESSION   DAOTGZ010000323 DAOTGZ010000000
VERSION     DAOTGZ010000323.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868088
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 2423)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2423)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:22:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,785
            CDSs (total)                      :: 2,751
            Genes (coding)                    :: 2,732
            CDSs (with protein)               :: 2,732
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 12 of 19
            Pseudo Genes (internal stop)      :: 5 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2423
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_51699_length_2423_cov_5.317145"
                     /isolate="AMR_MDS_3064"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..430)
                     /locus_tag="P5123_13515"
     CDS             complement(<1..430)
                     /locus_tag="P5123_13515"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRX17327.1"
                     /translation="MSNNRSKINKILVRQAYRSLSRGSLVEAVGCLEQVAADKISKTG
                     PYVDYLLAVAYLKSDRLAPASELVRNALRRYPEYAPFKEILAYLALKSGTSREQVIGP
                     IIQYEQEYTQSRKIRKLHKKLLSCRDFEVFQRKLKLEECIT"
     gene            complement(423..713)
                     /locus_tag="P5123_13520"
     CDS             complement(423..713)
                     /locus_tag="P5123_13520"
                     /inference="COORDINATES: protein motif:HMM:NF021542.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2273 domain-containing protein"
                     /protein_id="HRX17328.1"
                     /translation="MNQIYRHLKKFFERYPGRAYGASAGFVIGVLLLTIGFKRTLFIL
                     LITLLGFLIGFLIDSNMRIPGFPLRKKSNNGDDDLRVYYEDEDTDESGENIE"
     gene            complement(715..1278)
                     /gene="efp"
                     /locus_tag="P5123_13525"
     CDS             complement(715..1278)
                     /gene="efp"
                     /locus_tag="P5123_13525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013455316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor P"
                     /protein_id="HRX17329.1"
                     /translation="MISSNDFKKGMAIKINSDIFTIVDFQHVKPGKGAAFVRSKLKSL
                     TKGTVLDKTFRAGEKVEDIRVEKRGMQYSYDEGDSLVFMDTETYDEFRVNKDIIGNLL
                     DYIKEGDTVEISVYEEKPISIDPPIFVELLVTYAEPGVRGDTATNVNKPVTVETGAQI
                     HVPIFVNQDDIIKIDTRTGEYVERVKK"
     gene            complement(1293..2363)
                     /locus_tag="P5123_13530"
     CDS             complement(1293..2363)
                     /locus_tag="P5123_13530"
                     /inference="COORDINATES: protein motif:HMM:NF012766.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Xaa-Pro peptidase family protein"
                     /protein_id="HRX17330.1"
                     /translation="MKQINRLRDIIDKQGNFPFVIFDLKNIFYLTGFKGSSGYLVIDK
                     DNAWFITDSRYEEYARKLLTESGIDFVLQTGVVYETIKELVKNIGWKQIYVEDHFLTL
                     EMFNSLAETVSAKVRAGGNPCRDLRVVKDSSELALLRKAASITDNCMDHLCNIIKPGL
                     TEWDVAVELEHYYRTHGATGSSFDPIVASGANSSMPHHETSMTKKIVPGDTLLIDMGC
                     VYEGYNSDLTRTLFVGDISVTMREIYGIVNNARQAAIDSAIAGATTGELDSIARKIIS
                     DAGYGERFGHSLGHGVGLDVHEHPRVKGEDDFVLPAGSVITIEPGIYIPGTGGVRIED
                     LLHITDNGPELLSSSSREIRII"
BASE COUNT          734 a          548 c          389 g          752 t
ORIGIN      
        1 gtgttataca ctcttccaat ttaagctttc gttgaaaaac ttcaaaatcc cgacaggata
       61 aaagcttttt atgcagtttt cttatcttac gactttgagt atactcctgc tcgtattgta
      121 taatcggacc aataacctgt tccctgcttg ttcccgattt taaagcaaga taagccagta
      181 tctctttgaa aggagcatat tccgggtagc gtctcaaggc atttctaaca agctctgatg
      241 ctggtgctaa ccgatcactt tttagatagg caacagcaag aagataatcc acatatggcc
      301 ctgttttact tattttgtcg gcagcaacct gttcaaggca acccacagcc tcgactagag
      361 atccacgaga aagtgaacga tatgcctgac gtacaagaat tttattgatt tttgaacggt
      421 tattactcaa tattttcacc gctctcatcg gtatcttcat cttcgtagta gacacgaaga
      481 tcatcgtcac cattattgct cttttttctt aacggaaatc ccggtatacg catattactg
      541 tcgataagaa agccgattaa aaaaccaagt agggttataa gcaaaataaa aagtgtacgc
      601 ttaaacccaa tggtcagcaa taaaacaccg attacaaaac cggcgcttgc accgtatgca
      661 cgcccaggat atcgttcaaa aaatttcttt aggtgacgat atatctgatt cattctattt
      721 ttttacccgt tctacatact cacctgtacg agtatctatt ttaattatat catcctggtt
      781 aacaaagatt ggaacatgaa tttgtgctcc tgtctcaacg gttacaggtt tgtttacatt
      841 tgtggcagta tcgccacgaa ctcccggttc ggcgtaggtg accagaagtt ctacaaatat
      901 cggtggatca atggatatcg gcttctcttc ataaacagat atttctaccg tatcaccctc
      961 tttgatataa tcaagaagat tacctataat atccttgttt acacgaaatt catcatacgt
     1021 ttccgtatcc ataaaaacaa gactatcccc ttcatcataa gagtactgca taccgcgttt
     1081 ttcaactcga atatcctcga ccttttcacc agctctgaaa gtcttatcaa gtacagtacc
     1141 ttttgtaaga gactttaact tgctacgcac aaaggcagct ccttttccag gcttaacatg
     1201 ttgaaaatca acaatagtaa agatatcgct gtttattttt attgccatac cttttttaaa
     1261 atcattactc gaaatcattt ttacctctta aattaaataa tacgtatttc acgagaagaa
     1321 ctacttagta attccggacc attatcagtt atgtgaagaa gatcttcaat ccgaaccccg
     1381 cctgtccccg gaatatagat gcccggttca atagtaataa cagaacctgc tggcaaaaca
     1441 aaatcatctt ctcccttaac tcgaggatgc tcgtgaacgt caagtccaac gccatgcccc
     1501 agagaatgtc cgaaacgttc gccataaccg gcgtcactga ttattttacg ggcaatcgaa
     1561 tccagttcgc cggtagttgc cccagcaatc gcgctatcaa ttgccgcctg acgggcatta
     1621 ttaacaatgc cgtaaatttc acgcattgta acgcttatat ctccaacaaa cagggttctt
     1681 gtcaagtcag aattataacc ttcatacaca caccccatgt ctatcaacag agtatcacca
     1741 ggaactatct ttttagtcat ggatgtctcg tgatgtggca ttgaagagtt tgcaccagaa
     1801 gccacaatag gatcaaaaga gctcccggtt gctccatgag ttctgtaata atgttcgagt
     1861 tccactgcga catcccattc cgtaagccca ggcttgatta tattacatag atgatccatg
     1921 cagttatcgg ttattgaggc ggctttacgt aatagagcca attcagaact atctttaact
     1981 actcttaaat cacgacatgg atttccgcct gctcttactt ttgcagaaac tgtttccgca
     2041 agagaattaa acatttcaag tgtaagaaag tgatcctcca cataaatctg tttccatcca
     2101 atatttttga caagttcttt gattgtttca taaacaacac ctgtttgtaa aacaaaatca
     2161 attccacttt cggtcaataa ttttcttgca tactcttcat aacgtgaatc tgtaataaac
     2221 caagcattat ctttatcaat taccagataa ccacttgaac ctttaaaacc ggttaaataa
     2281 aaaatatttt ttagatcaaa tattacaaag ggaaaatttc cctgtttatc tataatatct
     2341 cgaagacgat taatctgttt catttttata tccatagtga gattttatta tagcagacgc
     2401 cctgtccaaa gctccacctc tat
//
LOCUS       DAOTGZ010000324         2397 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_3064
            NODE_52445_length_2397_cov_5.846712, whole genome shotgun sequence.
ACCESSION   DAOTGZ010000324 DAOTGZ010000000
VERSION     DAOTGZ010000324.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868088
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 2397)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2397)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:22:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,785
            CDSs (total)                      :: 2,751
            Genes (coding)                    :: 2,732
            CDSs (with protein)               :: 2,732
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 12 of 19
            Pseudo Genes (internal stop)      :: 5 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2397
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_52445_length_2397_cov_5.846712"
                     /isolate="AMR_MDS_3064"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..205)
                     /locus_tag="P5123_13535"
     CDS             complement(<1..205)
                     /locus_tag="P5123_13535"
                     /inference="COORDINATES: protein motif:HMM:NF021253.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="addiction module protein"
                     /protein_id="HRX17331.1"
                     /translation="MKVTIPVDKMSTNEKIEAMELLWDDLCKSTEKVPVLDWHKDILD
                     ERQIAVAEGREKAVDWETATAIWR"
     gene            complement(718..954)
                     /locus_tag="P5123_13540"
     CDS             complement(718..954)
                     /locus_tag="P5123_13540"
                     /inference="COORDINATES: protein motif:HMM:TIGR03831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YgiT-type zinc finger protein"
                     /protein_id="HRX17332.1"
                     /translation="MKINEQCRCGSSLESKYISHLIDFGSSVLVIRNVPALVCSRCGY
                     KLFDDVVAENLEKMEIDFKKRNDLIEVTNYKEVA"
     gene            complement(951..1271)
                     /locus_tag="P5123_13545"
     CDS             complement(951..1271)
                     /locus_tag="P5123_13545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012119498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4258 domain-containing protein"
                     /protein_id="HRX17333.1"
                     /translation="MDFQYNNIIEAVKKRRIRWSDHALEEKRADNLTIAQIKESLLQG
                     EIIEEYPSDKPLPSCLILGFAGDTPIHSVVGFNEENAYIRIITVYIPDTQKFEQNFKI
                     RRKK"
     gene            complement(1306..1875)
                     /locus_tag="P5123_13550"
     CDS             complement(1306..1875)
                     /locus_tag="P5123_13550"
                     /inference="COORDINATES: protein motif:HMM:NF019237.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1566 domain-containing protein"
                     /protein_id="HRX17334.1"
                     /translation="MPEPRVIVLLLDQQQLVIGPFLPSCLCLNDSTPPSLFDPLNGPI
                     IFVYLCKRCRIGQTAAGHRPDNIHTTNDDGSYQTGNDVPSTRFLDNGDGTVTDNMTGL
                     MWVKNANAIGTLHSGFDADGRVTWQNALTFVSEVNNGTYDCGITSVYTDWRLPTVCEL
                     KILTNYSQSAPYTWLKNHGFTAVFFLLIN"
     gene            complement(1904..2269)
                     /locus_tag="P5123_13555"
     CDS             complement(1904..2269)
                     /locus_tag="P5123_13555"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRX17335.1"
                     /translation="MHRDLRELYDIMYQLKDYLDNEKDLPLIMSFLIYMMSFTNITPE
                     RLKTVTRQISDKGGKIVMTTGMKLIEKGKLEGKLEGINEGITKGKAETAIAMIKESIP
                     LEMISRCTGLTEEEIASIK"
BASE COUNT          701 a          505 c          430 g          761 t
ORIGIN      
        1 agcgccagat agctgttgct gtctcccagt ctactgcttt ctctctacct tcagcaacag
       61 ctatctggcg ctcatcaaga atatccttat gccaatccag taccggcact ttttctgtgc
      121 ttttacacag atcgtcccat aatagttcca tggcttcaat tttctcgttt gttgacattt
      181 tatcaacagg tatggtgact ttcacgttat ctctccttta acaggataac tgacttttat
      241 ataagaatac aagtaattga aaggaagtca atgagaaaac agtgagtgtt agaacctcaa
      301 tctcgatatt tcgtctgtat aaaacgcccc ctcgatacaa ttgcggtctt tgagtaggac
      361 gaagtccata cttgtagtga gtttatcgaa ctatcgaaat gcggcaatca cacttcgaca
      421 agctcagtgc aagcaggctc ggcaaccaat aaacacctac ccagcaccat aatttcttta
      481 aattatggtg ctgcggagca cacaggccgc acgtagccgt tatccgtctt atcgctaaag
      541 ctcccgcgta cgcaaaacta tttattcatt atcacgccgg aaggcgatag atcttgtcgt
      601 atatacatcg agtgaatatg aagcattgaa aaaaatgaga acttcttttt acaatgagat
      661 tgaaagaacc ggcagagtgc tctatgaaaa agccggttga agattggctg acagctctta
      721 tgcaacttct ttataattgg ttacttcaat aagatcattt cttttcttaa aatcaatttc
      781 cattttttca aggttttcag caacaacatc atcaaagagt ttatatccgc atctcgaaca
      841 aaccaaagcc ggtacatttc ttataacaag cacagacgaa ccaaaatcaa ttaaatgaga
      901 aatatatttt gactccagag atgatccaca gcgacattgt tcgttgatct tcattttttt
      961 ctcctaattt tgaagttctg ttcaaacttt tgggtatcag gaatatatac agtaataatt
     1021 ctaatataag cattttcttc gttaaaaccc acaactgaat gtattggtgt atcacctgca
     1081 aaacccaata tcaaacagct tggtaatggc ttgtcagatg gatattcctc aattatttca
     1141 ccctgcaata gtgattcctt tatctgagca attgttaaat tgtctgctct tttttcttca
     1201 agagcgtgat cactccaccg aatacgacgc tttttcacag cttcgataat attgttgtat
     1261 tggaaatcca cattatcacc ttattacttt tatgttttaa cagctttaat ttatcaacaa
     1321 gaaaaagact gctgtaaagc catggttttt cagccatgtg taaggggcgc tctgactgta
     1381 gtttgtcagt atcttaagct cgcacactgt gggcagtcgc cagtcggtat agactgatgt
     1441 aatgccgcag tcataggtgc cgttatttac ctcagaaaca aaggtcaagg cattctgcca
     1501 tgtcacacga ccatccgcat cgaaaccgga gtgcagggtt cctatggcat tggcgttttt
     1561 tacccacata agccctgtca tattgtcggt gacggtaccg tcgccgttgt cgagaaaacg
     1621 cgtcgagggt acatcgttac ctgtttgata gctgccgtca tcatttgtcg tatgtatatt
     1681 gtctggccgg tgaccggcag ctgtttgccc gatccggcat cttttgcata aatatacaaa
     1741 aattataggt ccatttagcg ggtcaaacag agacggtggt gttgaatcat ttaaacagag
     1801 gcaggaaggt aaaaaaggac caatgactaa ctgttgttga tcaagaagta aaacaataac
     1861 acgaggttcc ggcacaaggc cggaacgaca ttaaacgaag ttatcattta atcgaagcaa
     1921 tctcttcttc tgtaagaccg gtacatcgag atatcatttc taatggaata ctttctttaa
     1981 tcatggctat ggctgtttct gctttacctt ttgtaatacc ttcgttgata ccttccagct
     2041 taccctcaag tttacctttt tctattaatt tcattccagt tgtcattaca attttacctc
     2101 ctttatctga tatttgtcga gttacagtct ttaagcgttc aggtgtaata tttgtaaaac
     2161 tcatcatata aattaaaaaa gacattataa gcggtaaatc tttttcgtta tccagataat
     2221 ctttcaactg atacattata tcgtacagct cgcgtaggtc acgatgcatc gtgccctact
     2281 acacaccggt gtattattaa taagaattga tctctttttt tgttagacca gtacatttag
     2341 atattaattc atcgtcgtag cgaaaaatat cagtcaaaat ataactaaaa tctgaaa
//
LOCUS       DAOTGZ010000325         2389 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_3064
            NODE_52675_length_2389_cov_3.736504, whole genome shotgun sequence.
ACCESSION   DAOTGZ010000325 DAOTGZ010000000
VERSION     DAOTGZ010000325.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868088
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 2389)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2389)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:22:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,785
            CDSs (total)                      :: 2,751
            Genes (coding)                    :: 2,732
            CDSs (with protein)               :: 2,732
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 12 of 19
            Pseudo Genes (internal stop)      :: 5 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2389
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_52675_length_2389_cov_3.736504"
                     /isolate="AMR_MDS_3064"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..270
                     /locus_tag="P5123_13560"
     CDS             <1..270
                     /locus_tag="P5123_13560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006829824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HRX17336.1"
                     /translation="IVNSRGARITVRGIFHIVVRRARKSGFTSSVTPHALRHSFATEL
                     LNNGADIKAVQDMLGHSSISTTQIYTHTTRARLKQVYQQFHPHAD"
     gene            617..922
                     /locus_tag="P5123_13565"
     CDS             617..922
                     /locus_tag="P5123_13565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002771874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRX17337.1"
                     /translation="MSLDFEKDFNDEVYEDSFYSEDEEELEEDDELVKDELEDEDEED
                     EIEDPVGAVRIDVSFVCDDCDYRWDDTIVQKNNEYNEAEDITDCVCPMCGSMNVAQI"
     gene            906..>2389
                     /locus_tag="P5123_13570"
     CDS             906..>2389
                     /locus_tag="P5123_13570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018132063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrA"
                     /protein_id="HRX17338.1"
                     /translation="MSHKYNSFIHIRGAREHNLKNISIDIPRDSLVVITGLSGSGKSS
                     LAFDTIYAEGQRRYVESLSAYARQFLGIMEKPDVDFIDGLSPAISIEQKSTHRNPRST
                     VGTVTEIYDYLRLLYARIGIPYCPSCNIKITSRSVDQIVGQLLTYADKTKMQLLSPVV
                     QGKKGTHEDTFESLRRKGFVRLRIDGEARTLDEEIKLDKNKKHNIEVIVDRIVIKDGV
                     RPRLADSVETALDLSEGVLVADIDGNELFFSSKHACPECGYSIPELSPRMFSFNSPFG
                     ACEECSGLGFLMRFSEDLIVPDHSVTLDDGAVQVWGKQWTSEQVKFLQRNFNIDTSKP
                     WKKLTKKEQNMVLYGTGDRESRRYPFNRNFEGAIPNLERRYKETNSEDMRHWMESYMV
                     NQPCPACNGNRLKPASLAVRVAETHISDITRLSIKEAFDFFENLIISDTERKIAVQVL
                     QEISLRLKFLKDVGLDYLALERAAGTLSGGEAQRIRLATQIGSS"
BASE COUNT          716 a          416 c          532 g          725 t
ORIGIN      
        1 atcgtgaatt ctaggggggc gagaataacg gttcgcggta tttttcatat agtggtgcgt
       61 agagccagaa agagtggttt cacatcgtcc gtaactccgc atgcattacg ccatagtttt
      121 gcaaccgagt tacttaacaa cggtgctgat attaaggctg ttcaggatat gcttggacat
      181 agctcgatat ctactactca gatatacact cacacaacac gggcgagact gaaacaggtt
      241 tatcagcagt ttcatcctca tgccgattaa acatatcaga ttttaaatac aattttttta
      301 gcttaataac ttaaaaaatg ctttaaaaag agttaaaagt agttaatata tatttaatga
      361 ataacaaaat tatttattgt acttctgtta ttttcaatgt tattaaaaca tcaagatgat
      421 ttgctgttca gtacgtacgt atggatttta tatttgttta atgcttgtaa gttttataaa
      481 cataaaaaag cttataaatc tgtttgacaa atatacttag gtttttttct ttaaaacacg
      541 gtatttgtga ttaattaaat atcttacgtt tgcctttttt agggaaacga tattcttgaa
      601 tgagtcaggt aaaagtatga gtttggattt tgaaaaagat tttaatgatg aagtttatga
      661 ggacagcttt tacagcgaag acgaagaaga acttgaagaa gacgatgaac ttgtaaaaga
      721 tgaacttgaa gatgaggatg aagaagatga gatcgaggat cctgtcggcg ccgtccgcat
      781 agatgtctcc tttgtatgcg atgattgtga ttatcgttgg gatgacacca tagttcaaaa
      841 aaataacgag tataacgagg ccgaagatat cactgattgt gtttgtccaa tgtgcggttc
      901 gatgaatgtc gcacaaatat aacagtttta tccatattcg cggtgcccgt gagcataatc
      961 ttaagaatat atcgatagat attccccgag acagtcttgt ggttatcaca ggattgtcag
     1021 ggtcaggtaa atcttcattg gcttttgata cgatctatgc ggaagggcag agaagatatg
     1081 tagagtcttt gtcagcttat gccaggcagt ttcttggtat tatggaaaaa ccggatgtag
     1141 attttattga tggcctttcc ccagcgattt ctattgaaca aaagagtact caccgtaatc
     1201 cgcgatcaac cgtgggtact gtgaccgaga tttatgacta tttgaggctt ctgtacgctc
     1261 ggataggcat tccttactgc ccctcatgta atataaaaat tacttctcgt tcagttgatc
     1321 agatagtcgg gcagctttta acttatgcag ataagacaaa aatgcaacta ttatctccag
     1381 ttgtgcaggg caagaaaggt actcatgaag ataccttcga atcactcagg cgaaaaggtt
     1441 ttgtgcgttt acgtatcgac ggagaagctc gtaccttaga cgaagagata aagctcgata
     1501 agaataaaaa acacaacata gaagtaatcg ttgatcgtat agtaatcaaa gacggtgtgc
     1561 gtccacgcct cgctgattcg gttgagaccg ctctggatct ttctgaagga gtgcttgtcg
     1621 ctgatattga tggaaacgag ctcttcttct cttcaaaaca cgcctgtcct gaatgtggtt
     1681 attccattcc tgaactttca ccgcggatgt tttcgtttaa cagtcctttt ggtgcgtgcg
     1741 aagagtgcag cggcctgggg tttcttatgc gatttagtga agatctgatt gtaccggacc
     1801 attctgtaac ccttgatgat ggggctgtgc aggtttgggg caagcagtgg acctcagagc
     1861 aggttaagtt tttacaacgc aacttcaata tagacacctc aaaaccctgg aaaaaactta
     1921 caaaaaaaga acagaatatg gttctttacg gaaccggaga tcgagaatca agacgttacc
     1981 catttaatag aaatttcgag ggtgcaatac caaatcttga acgtcgttat aaagagacaa
     2041 attccgagga tatgcgtcat tggatggaga gctatatggt taaccagcca tgtcctgctt
     2101 gtaatgggaa caggcttaaa ccagcatctc tggctgtgcg tgttgctgaa acacatattt
     2161 cagatataac cagactttca ataaaagagg cctttgattt ttttgaaaac ctgattattt
     2221 cggataccga aagaaagatt gctgtccagg ttttacagga gattagctta aggctcaagt
     2281 tcttaaagga tgtgggtctt gattatcttg ctctcgagcg tgcggctggt actttgtcgg
     2341 ggggagaagc tcaacgaatc agacttgcca ctcagattgg atcaagttt
//
LOCUS       DAOTGZ010000326         2369 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_3064
            NODE_53269_length_2369_cov_3.516854, whole genome shotgun sequence.
ACCESSION   DAOTGZ010000326 DAOTGZ010000000
VERSION     DAOTGZ010000326.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868088
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 2369)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2369)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:22:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,785
            CDSs (total)                      :: 2,751
            Genes (coding)                    :: 2,732
            CDSs (with protein)               :: 2,732
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 12 of 19
            Pseudo Genes (internal stop)      :: 5 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2369
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_53269_length_2369_cov_3.516854"
                     /isolate="AMR_MDS_3064"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1169
                     /locus_tag="P5123_13575"
     CDS             <1..1169
                     /locus_tag="P5123_13575"
                     /inference="COORDINATES: protein motif:HMM:NF014026.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="glycoside hydrolase family 3 C-terminal
                     domain-containing protein"
                     /protein_id="HRX17339.1"
                     /translation="SRIPFEKNDHPDHHKLSLESARRSMVLLKNNDILPLKKDKLKSI
                     AVIGPNADDPVMLLGNYSGTPSYTTTPLRAIKDALGDTARVYYAQGCDISKDRVEPLA
                     TEDDRVAEAVSVAKASDVAIVCLGLNSQIEGEQGDVGNSDAGGDKINLSFPGMQNKLL
                     EKIVATGTKTIVAVFAGSAMDLTYANDNADAVIQAWYPGPMGGEAFADLLLGKSDFSG
                     RLPVTFVKKTEDLPDFRDYSMEGRTYRFIKTDPLYPFGYGLSYNNYEYSGLKIEKDVV
                     EQGESVNVSIKVTNKGKFDGYGSVQVYLKDVDATVRVPNYSLAAFKSVYLKAGEKKEV
                     SLVVDSNCMVVFDDDGSTRFEPGLFEIYVGGGAPDSVSCKLMGSSPLKAEFELK"
     gene            complement(1263..1910)
                     /locus_tag="P5123_13580"
     CDS             complement(1263..1910)
                     /locus_tag="P5123_13580"
                     /inference="COORDINATES: protein motif:HMM:NF013933.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysE family transporter"
                     /protein_id="HRX17340.1"
                     /translation="METLVLIFFTSFGVAFSGALMPGPLLTVTISESTKRGMSAGPLL
                     VAGHAVLEMALIVALVFGLAPVFKIDLFFISVAFAGGLAMIIMATGMFRSLSTLTVNN
                     VNDINKSDTPASGNLIFKGIIMSLANPYWTIWWATIGIGMIILSKNLGLAGVVTFFLG
                     HILGDLVWYAAVSIAISKGRRLFTDKMYRGIIATCAVYLTGFAVFLIFSGIRKLI"
     gene            complement(1941..2165)
                     /locus_tag="P5123_13585"
     CDS             complement(1941..2165)
                     /locus_tag="P5123_13585"
                     /inference="COORDINATES: protein motif:HMM:NF023251.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3343 domain-containing protein"
                     /protein_id="HRX17341.1"
                     /translation="MIDVILFHSSSEAMWASRVLKKNKIEHKLISVPRHLGSDCGYCV
                     SLSKTDSPNAMEFIRTEKVPFDRLEQNVTI"
     gene            complement(2162..>2369)
                     /locus_tag="P5123_13590"
     CDS             complement(2162..>2369)
                     /locus_tag="P5123_13590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007623726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRX17342.1"
                     /translation="ILRRIAIVWCSSLVIWIPAVSIIYSLPQNLQLPLFNIILCFFAL
                     ILALVAKGTEKSELEIHKTGTILP"
BASE COUNT          725 a          453 c          535 g          656 t
ORIGIN      
        1 attcacggat acctttcgaa aagaatgatc atcccgatca tcataagctc tctctcgaat
       61 ccgcacgacg ttcgatggta cttttaaaaa ataatgatat attaccgtta aaaaaagata
      121 aactgaagag tattgctgtt atagggccta acgccgatga ccccgtaatg cttcttggta
      181 actatagcgg aacgccttca tacacaacta cccctttgag agcgattaag gatgcgcttg
      241 gagatactgc gcgtgtatat tacgctcaag gatgtgatat cagcaaagac cgtgttgaac
      301 cgttggcaac cgaagatgat cgagtagccg aggctgtaag cgttgcaaaa gcaagtgatg
      361 tcgctatagt ttgtcttggg ctcaattctc agattgaggg cgaacagggt gacgttggta
      421 actcggatgc cggtggagac aagatcaatc tctcttttcc aggaatgcaa aacaagctgc
      481 tcgaaaaaat agttgcgacc ggtacaaaga caattgttgc ggtatttgcc ggcagtgcca
      541 tggatttaac ttacgctaac gataatgcgg atgccgttat acaagcatgg tatccaggcc
      601 caatgggagg tgaggctttt gctgatcttc ttttgggtaa gtccgatttt agcggacgtc
      661 ttccggtaac ctttgtcaag aagacagaag atttgcctga ttttcgtgat tatagtatgg
      721 aggggcgtac ttaccggttt ataaaaactg atccacttta tccatttggc tacgggctta
      781 gttataataa ttatgaatac tccggactta aaattgaaaa agatgttgtt gaacagggag
      841 aatcagtcaa tgtttcaatc aaggttacta acaaaggaaa atttgatggc tatggatcgg
      901 ttcaggtcta tttaaaagat gttgatgcta cagtaagggt tcccaattat tctctggctg
      961 cttttaaaag tgtttaccta aaggcaggcg agaagaaaga ggtaagcctt gttgtcgatt
     1021 caaattgcat ggtggttttt gatgatgatg gcagtaccag gttcgagccg ggactgtttg
     1081 agatttacgt ggggggaggc gctcccgact ctgttagctg taaactaatg ggcagctctc
     1141 cgcttaaggc tgaattcgaa ttaaaatagt tagtttgcag agatatcatt gatcgtagaa
     1201 aatggtggcg ttcgcaaggg cgccgccatt attatctgtt aaaaaattta aagtcgaggt
     1261 gcttaaataa gttttcttat acccgaaaat ataagaaaga ccgcaaaacc tgtaagataa
     1321 acagcgcaag tggcaataat tcccctgtac attttgtcgg tgaacaatct tcttcctttg
     1381 cttatcgcga tagaaacagc agcataccag actaggtctc ccagaatgtg gcccagaaaa
     1441 aaagtaacta ccccagctaa acccaggttt tttgataaaa ttatcatacc tatgcctatg
     1501 gtagcccacc atattgtcca gtagggattt gctaggctca taataatgcc tttgaaaata
     1561 aggttgccac ttgccggtgt gtcggattta ttaatatcgt taacattgtt aacggtaagc
     1621 gtcgaaagag agcgaaacat tccagttgcc ataataatca tggcaagtcc tccagcgaaa
     1681 gcaacagata taaaaaaaag atcaatttta aaaacaggag ctaatccgaa gaccagagca
     1741 acaattaatg ccatctcaag taccgcgtga cctgctacaa gtagcggtcc cgctgacatt
     1801 ccacgttttg tagattcact tatggtaaca gttaaaagtg ggccaggcat tagagctccc
     1861 gaaaaggcaa ctccaaaaga tgtgaagaaa ataagtacta atgtctccat gacgactcca
     1921 tactgttcaa taaatatgat ctaaattgtt acattttgtt ccaatctgtc aaacggaact
     1981 ttttctgtgc gtataaattc cattgcgttt ggtgaatctg ttttggatag ggaaacgcag
     2041 taaccgcagt cagaaccgag atgccgggga acagagatca gtttatgttc aattttattt
     2101 ttttttaaaa cacgggaggc ccacatggct tcgctggagc tatggaaaag tataacatca
     2161 atcatggcaa aatggttcct gttttatgaa tttcgagctc agatttttcc gtgccttttg
     2221 ctaccagagc caaaataagc gcaaaaaaac agagaataat attgaaaagt ggaagttgca
     2281 ggttttgcgg caggctgtat atgattgaaa cagccggtat ccagattaca agactcgaac
     2341 accatacaat ggcaatgcgc cgtaatatt
//
LOCUS       DAOTGZ010000327         2367 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_3064
            NODE_53337_length_2367_cov_3.705882, whole genome shotgun sequence.
ACCESSION   DAOTGZ010000327 DAOTGZ010000000
VERSION     DAOTGZ010000327.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868088
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 2367)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2367)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:22:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,785
            CDSs (total)                      :: 2,751
            Genes (coding)                    :: 2,732
            CDSs (with protein)               :: 2,732
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 12 of 19
            Pseudo Genes (internal stop)      :: 5 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2367
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_53337_length_2367_cov_3.705882"
                     /isolate="AMR_MDS_3064"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..134)
                     /locus_tag="P5123_13595"
     CDS             complement(<1..134)
                     /locus_tag="P5123_13595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009437906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Tex-like N-terminal domain-containing protein"
                     /protein_id="HRX17343.1"
                     /translation="MNIYGYIADQHKLTEKQVVSVVRLLDDGATIPFMARYRKEATGA
                     L"
     gene            complement(234..542)
                     /locus_tag="P5123_13600"
     CDS             complement(234..542)
                     /locus_tag="P5123_13600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012648361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase domain-containing
                     protein"
                     /protein_id="HRX17344.1"
                     /translation="MYGLSDDVLEKLRNIFSEFENIEKVILYGSRAKGNYKSGSDIDL
                     TLVGRELTLANCLYPVIDRIEMLLLPYMVDLSIFTQISNPDLIEHIDGVGKIIYQRSD
                     "
     gene            complement(544..963)
                     /locus_tag="P5123_13605"
     CDS             complement(544..963)
                     /locus_tag="P5123_13605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018968897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase substrate binding
                     protein"
                     /protein_id="HRX17345.1"
                     /translation="MNEDTRWLQRFANYRKALQPLNMAVNLAGSRDLSDLDKQGFIQA
                     FEFTYELAWNVVNDFYESKGEINIQGSIDAFRLAFNRGLVSKGEALLNAVKSRRLTNH
                     TYDEKVAEEIFNSIKNSYIHAFNELESKLLIEEEKDN"
     gene            complement(1030..2220)
                     /locus_tag="P5123_13610"
     CDS             complement(1030..2220)
                     /locus_tag="P5123_13610"
                     /inference="COORDINATES: protein motif:HMM:NF019310.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HRX17346.1"
                     /translation="MKIKKTDIYFLLLFLENYFFFSSNAAINLLPPYLEKLGASHAFT
                     GFFMNINSLTLVVAVVLFSHYIPRLDRKRTLNTVFFISMVANFIMFIFHNNLHVLAVL
                     RFAASFAYLFGFTMHANMAFELIPRHRRAGGIALFGLSGVFSNPTGAVLGELIINNFS
                     YRGLFVLAFCFSTGAFFLSMFIREGVASVTERSSRNFFSILMRKGSRSLYLYGFLLGG
                     AFSTFATFLPGHSKASLGFANLSYFFVSHGVVSIVMRIFFNKWMDRLRPSLLVSVGLF
                     SASIAMFVSLNLVAYNQLIISGLFYGLGHSILYPLLGTLIIHTGSDGLKDTLNNAYIA
                     SYTLGCVGITTLLGVLADLSDLKYVFLVKGIVLFVNSLTALYFIRKIHQQRFGVCPAV
                     AEEE"
BASE COUNT          823 a          488 c          421 g          635 t
ORIGIN      
        1 agggccccgg tcgcctcttt tcgatacctt gccataaagg gaattgtcgc tccatcgtca
       61 agaaggcgaa ccaccgacac aacctgcttt tctgtaagtt tatgctgatc cgcaatatat
      121 ccatatatat tcattatagg aattctcctg tttgtaatct tattcaatgt gacataatgc
      181 ggcaaattta gacaagaata attttgtatt ttgtaataca agttttgaca ggattaatca
      241 gatcgctgat aaatgatttt tccgacaccg tcaatatgct caatcagatc cggattgcta
      301 atctgagtga agatggacag atctaccata taaggcaaca gaagcatttc tatcctgtca
      361 ataacaggat atagacaatt tgcaagagtc agctctcgtc caacaagagt cagatcaata
      421 tcagagcccg atttataatt accttttgcc ctggatccat aaagtataac cttttcaata
      481 ttttcaaact cggagaagat gtttcgtaat ttttccagaa catcatcgct taaaccatac
      541 acattaatta tctttctctt cttcaattaa tagttttgat tctagttcat taaacgcatg
      601 gatataagaa tttttaattg aattaaaaat ctcttcggca accttttcat cataagtatg
      661 gtttgttaaa cgcctgcttt ttacagcatt taacagtgct tcacctttcg agacaagccc
      721 cctgttaaaa gccaatctga aagcatcaat gctcccctga atattgatct ctccttttga
      781 ttcataaaaa tcattcacaa cattccaggc aagttcatac gtgaactcaa aagcctgtat
      841 aaagccctgt ttatcaagat ctgacagatc acggctaccc gcaagattga cagccatatt
      901 taaaggctgt aacgcttttc tataattcgc aaaccgttga agccagcggg tatcttcatt
      961 cattatcgga ttctccttta catatctaat acctgtttta atcaagtcaa caaggccggc
     1021 taaacaaatc tactcctctt cagcgactgc cggacacact ccgaaacgct gctggtgaat
     1081 tttacgtata aaatagaggg ctgtcaatga attaacaaat agaacaattc ctttaaccaa
     1141 aaatacatat ttcaaatctg acaggtcggc taagactcca agaagagttg ttatgccaac
     1201 acatccaagg gtatacgacg cgatataggc attgttcagc gtgtctttaa ggccatcgct
     1261 gccggtatga attatcagtg taccaagaag aggatacaaa atggagtgcc caaggccata
     1321 aaacagacca gaaataatta actgattata tgcaacaaga tttaaagata caaacatcgc
     1381 aatcgaagca gagaagagcc caaccgatac aagcagagat ggtctaagac gatccatcca
     1441 cttgttaaaa aatattctca taactattga tacaacacca tgcgaaacaa agaaataaga
     1501 gagatttgca aacccaagag atgcctttga gtgtcctggc aaaaatgtcg cgaacgtgct
     1561 aaacgctcca ccaagaagaa agccgtaaag ataaagagac cgagatcctt ttcgcattaa
     1621 tatagaaaag aaattacggg atgatcgttc tgttactgaa gccacacctt ctcttataaa
     1681 cattgataaa aagaatgccc ctgttgaaaa gcaaaacgca agtacgaaaa gaccgcgata
     1741 cgaaaaatta tttattatca attcgcccag aaccgctcca gtcggattcg agaatactcc
     1801 tgacaagcca aaaagcgcaa ttcctccggc acgacgatgt cgaggtatca attcgaaagc
     1861 catatttgcg tgcattgtga aaccaaatag ataagcaaaa gatgccgcga aacgaagcac
     1921 tgccagaaca tgaagattgt tatgaaaaat gaacataata aaattggcta ccatgcttat
     1981 aaaaaaaaca gtgttaagtg tgcgtttacg gtcaagacgt ggtatatagt ggctaaaaag
     2041 cacaacagcc acaacaaggg ttaatgagtt tatattcata aaaaagccgg taaaagcgtg
     2101 agaagcgccg agcttttcca gatagggcgg aagaagatta atagccgcat tactggaaaa
     2161 gaaaaaatag ttttccaaaa atagaagaag gaaatatata tcggtttttt tgattttcaa
     2221 gctctgcttc catttgcttc cagatttata caattagtta tataaaaaaa cgacagcata
     2281 atgcaaatca aaaaaatgaa ccacatagtc caaacaattg cgcaatcttc aagcgcgatt
     2341 gtttggacgg tttcgggcga atagccc
//
LOCUS       DAOTGZ010000328         2347 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_3064
            NODE_53915_length_2347_cov_5.201134, whole genome shotgun sequence.
ACCESSION   DAOTGZ010000328 DAOTGZ010000000
VERSION     DAOTGZ010000328.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868088
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 2347)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2347)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:22:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,785
            CDSs (total)                      :: 2,751
            Genes (coding)                    :: 2,732
            CDSs (with protein)               :: 2,732
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 12 of 19
            Pseudo Genes (internal stop)      :: 5 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2347
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_53915_length_2347_cov_5.201134"
                     /isolate="AMR_MDS_3064"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..408
                     /locus_tag="P5123_13615"
     CDS             <1..408
                     /locus_tag="P5123_13615"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRX17347.1"
                     /translation="IPMKMKYFVLIFGTIEFFGTLNSINGQMGRISHVAHFGGLLAGF
                     ILLKTGMSFKSGEPFRANTPKKINFISEYFKKKRVENKRVEIDKRIKAKAIIDKMLEK
                     IAASGLESLTPEERRDLEWARKNYFPGGTDTIH"
     gene            536..608
                     /locus_tag="P5123_13620"
     tRNA            536..608
                     /locus_tag="P5123_13620"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:569..571,aa:Lys,seq:ttt)
     gene            633..705
                     /locus_tag="P5123_13625"
     tRNA            633..705
                     /locus_tag="P5123_13625"
                     /product="tRNA-Glu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:667..669,aa:Glu,seq:ttc)
     gene            960..>2347
                     /locus_tag="P5123_13630"
     CDS             960..>2347
                     /locus_tag="P5123_13630"
                     /EC_number="4.1.1.32"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013314927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004611 - phosphoenolpyruvate
                     carboxykinase activity [Evidence IEA];
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate carboxykinase
                     domain-containing protein"
                     /protein_id="HRX17348.1"
                     /translation="MLELKKGVDIISEVGGIKTMDEANAIFKKKMDDANLAKIAKIKT
                     EDAVIRIANAISMCDPDDVFIISDKEEDYRHVKKRSLETGEEAPLAIKDHTIHFDLPQ
                     DQGRLVKQTFYIVNEGEKTSSIAKTELRAESHKYIKENMTGIMKGLTMYVAFINRGPV
                     GASATIPAIMITSSTYVQTSCELLYRNGFSDFDAEVKRAGVFFTNCHSQGPNRPEDVP
                     NARIYMDRSWQTTYSTFCTYAGNTLLLKKGNHRFACDWAVYQKTGKELSEHMFITGLT
                     GPNGRKTYFAGAAPSGCGKTTTAMVGTDFIGDDLAQMWIDNGGLVRAVNPERGIFGIV
                     EDVNYEGDPYLMKRLREEGTEVIFSNVLIDENKKPRWVGDGEPVPAKGVNFQGEWTPE
                     MKDVPMSHPNSRCTLKNTAIDNYNEKDAEDSKGVQVKVVTYSGRDSDTMPPVWVAKSM
                     DAGVAIGASIVSA"
BASE COUNT          701 a          435 c          525 g          686 t
ORIGIN      
        1 attccgatga agatgaagta ttttgtgctt attttcggaa ccatcgagtt tttcggtaca
       61 ttaaattcaa taaacggtca gatgggtcga ataagccatg ttgcccattt tggtggattg
      121 ttagccgggt ttatcctctt aaaaacagga atgagcttta aaagcggcga gccctttcgt
      181 gctaatacac caaaaaaaat aaattttatt tcggaatatt ttaaaaagaa aagagttgaa
      241 aataaacgag ttgagatcga taagcgtata aaagctaagg ccattattga caagatgctt
      301 gaaaagattg cggcttcggg tcttgagtcc ttgacaccag aagagagacg cgatcttgag
      361 tgggcacgta agaactattt tcctggtggt actgacacaa ttcattaaca ctagttataa
      421 ttttcacgtt tgagtagaat tataagacag aaaaactgtt gtgaataaaa aactttcgta
      481 aaaatctaaa tttgtttgac acaaatatgc tctgttagta catggtacag acgttgaggc
      541 gctagctcag tcggtagagc aactgccttt taagcagtgg gtccggggtt cgatcccccg
      601 gcgcctcaaa tcccgtaagg gtttaacata atgtccccgt cgtttagtgg tctaggacac
      661 cgggttttca tcccggcaac aggggttcga accccctcgg ggatgcataa tgagccgcca
      721 taaggcggct cattattatt ttgttccgct ttttataaac gccttttacc ccgttttcag
      781 aactgttctg aagacaacgg tcttcctttg tccacactaa actttttttc ttgacaattt
      841 acccctatcc tttcgattaa gataaaaccg cttgtatgca gttgatactg ttattttgca
      901 ggtatcttga tttaattctg atagtagcgg ttttaaaaaa tcctagatag gagtaaagta
      961 tgttagaatt gaaaaaagga gtcgatatta tctcggaagt gggcgggata aaaacgatgg
     1021 atgaagcaaa tgccattttc aagaaaaaaa tggatgatgc taaccttgca aaaattgcaa
     1081 aaattaaaac agaagacgct gttatacgta tcgcgaacgc gatatcaatg tgtgatcctg
     1141 atgatgtttt tattatcagt gataaagagg aagactaccg tcatgtaaaa aaacgcagcc
     1201 ttgaaacggg tgaagaggcg cctcttgcta ttaaagacca tactatacat tttgaccttc
     1261 ctcaggatca gggacgtctt gttaaacaga ctttttacat tgtaaacgaa ggcgagaaga
     1321 caagttcaat agcaaaaaca gaacttcgtg ctgagtctca taaatatatt aaagagaata
     1381 tgaccggtat aatgaaaggg cttacaatgt atgttgcttt tattaaccgt ggaccagttg
     1441 gtgcttcagc aactattcct gcgataatga ttacatcatc aacttacgtt cagacttcgt
     1501 gtgagctttt gtatcgtaac ggattctctg attttgatgc agaggtaaag cgtgctggtg
     1561 tattctttac aaactgtcac tctcagggac caaaccgtcc ggaagatgtt cctaatgccc
     1621 gaatctatat ggacagaagt tggcagacaa cctattcaac tttctgtacc tatgcaggta
     1681 acactcttct tcttaaaaag ggtaatcacc gttttgcatg tgactgggcc gtttaccaga
     1741 agactggtaa agagctttca gagcatatgt ttattaccgg tcttacaggg ccaaatggcc
     1801 gcaagaccta ttttgctggc gctgctccat caggttgtgg taaaactaca acagcaatgg
     1861 taggaaccga ttttataggt gatgaccttg cacagatgtg gatcgacaat ggcggtcttg
     1921 ttcgtgcggt taaccctgaa agaggtatct tcggtattgt tgaagatgtt aactatgaag
     1981 gtgatccata tcttatgaag cgtcttcgtg aagagggaac agaagtaatt ttctcaaacg
     2041 tacttataga cgaaaacaag aaacctcgtt gggtcggaga tggcgagcct gttcctgcaa
     2101 agggtgttaa ctttcagggc gagtggactc cagagatgaa agatgttcct atgtcgcatc
     2161 caaactctag atgtactcta aaaaacacag ctattgataa ctataacgaa aaagatgccg
     2221 aagattcgaa aggtgttcag gttaaggtag taacttattc aggtcgtgat tctgacacta
     2281 tgcctcctgt ttgggttgcc aaatctatgg acgcgggtgt tgcgatcggt gcttctattg
     2341 tttcagc
//
LOCUS       DAOTGZ010000329         2344 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_3064
            NODE_54027_length_2344_cov_4.554827, whole genome shotgun sequence.
ACCESSION   DAOTGZ010000329 DAOTGZ010000000
VERSION     DAOTGZ010000329.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868088
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 2344)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2344)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:22:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,785
            CDSs (total)                      :: 2,751
            Genes (coding)                    :: 2,732
            CDSs (with protein)               :: 2,732
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 12 of 19
            Pseudo Genes (internal stop)      :: 5 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2344
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_54027_length_2344_cov_4.554827"
                     /isolate="AMR_MDS_3064"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..252)
                     /locus_tag="P5123_13635"
     CDS             complement(<1..252)
                     /locus_tag="P5123_13635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005951625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016836 - hydro-lyase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate hydratase C-terminal domain-containing
                     protein"
                     /protein_id="HRX17349.1"
                     /translation="MEISLTVPFNQDDLEKLRAGDEVLLSGVIYTARDAAHKRFITAL
                     DSGMELPLDLSGQVLYYTGPTPARPGQVIGSAGPTTSYRM"
     gene            complement(262..1110)
                     /locus_tag="P5123_13640"
     CDS             complement(262..1110)
                     /locus_tag="P5123_13640"
                     /EC_number="4.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007062414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate hydratase"
                     /protein_id="HRX17350.1"
                     /translation="MRNIDYNEIVETVAHACINSNIRLPECTMQYYRTALKRETDGRS
                     QGIIDKYLQNVEIADKQSLPLCQDTGTAVFFVTIGVDVKITGGPTLLVSDAITEGVRK
                     GYTEGYLRKSIVSDPLFDRKNSGDNTPPIIHINLCEEDILKIEYAPKGGGAENMSRLK
                     MMTPADGDDGVVDFVAETVKNAGGNPCPPVVIGVGIGGNFERCAILAKKALFREEPHP
                     DSRYAMLEQRILKAVNALGIGPQGFGGNVTAFDVHIEYESCHIASLPVAVNINCHVHR
                     HALINL"
     gene            complement(1107..1823)
                     /locus_tag="P5123_13645"
     CDS             complement(1107..1823)
                     /locus_tag="P5123_13645"
                     /inference="COORDINATES: protein motif:HMM:NF010071.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate reductase iron-sulfur subunit"
                     /protein_id="HRX17351.1"
                     /translation="MYKRVKILRYKPGSENKPYFESYDVEPDGDTILSLLQQIQKYID
                     PSLKFDYVCRSGVCGSCAMLINGKPRLACRTCLNEVKGSVTIAPLPGFLMLGDLSVDT
                     GSYFIKIHNRQALNVNRSKQQEAELAANSNYENELMMHLYELDRCIECGCCVSACATV
                     QMNADFAGAAELLNAWRLMSDPRDGRANKDWYELYATDNGVFGCTALLACDSYCPKNI
                     PLASSLASFRVALLKLGVKL"
     gene            complement(1816..>2344)
                     /locus_tag="P5123_13650"
     CDS             complement(1816..>2344)
                     /locus_tag="P5123_13650"
                     /inference="COORDINATES: protein motif:HMM:NF014907.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRX17352.1"
                     /translation="TVVSGELAAEGMCEYFSNNGYTPSAAVITKEYKGQCDRLVKLEE
                     KAGSNGVTFSELTFDLRSVMSRYAGVFRNEEGLKLAKKELCRILEKVDGAVVSKNFYS
                     MSPEIENALECRGMILLAMTVVEGALARKESRGCHNRDDYPDMSEEFQKRTLTRWNND
                     TQSVMIDYEDVSLHV"
BASE COUNT          684 a          598 c          407 g          655 t
ORIGIN      
        1 cattctgtac gatgtcgtcg ggcctgcact accaataacc tgtccaggac gcgcgggcgt
       61 tggccccgta taatacaaaa cctggccgct caaatcaagg ggcaactcca tgccggaatc
      121 aagagcagta ataaatcttt tatgtgctgc atctcgggca gtataaataa caccggaaag
      181 aagcacctca tccccagctc taagtttttc cagatcatcc tgattaaacg gtacagtcag
      241 tgaaatttcc attaatttct cctacaagtt aatcaaggca tggcgatgca catgacaatt
      301 gatatttacg gctacgggta aagaagctat atggcaagat tcatattcga tatgaacatc
      361 aaaagcagtt acattacctc caaaaccctg agggccaata ccaagcgcgt taactgcttt
      421 taatatacgc tgctctaaca ttgcgtaacg tgaatccggg tgcggctcct ctctgaaaag
      481 cgctttcttg gccaaaatag cgcatcgctc aaagttacca cctatcccaa caccaattac
      541 aacaggcggg cacggattgc ccccggcgtt tttaacagtc tcggcaacaa agtcaactac
      601 accgtcgtct ccatctgccg gtgtcatcat tttaagacga ctcatattct ccgcccctcc
      661 accttttgga gcatactcaa ttttaagaat atcttcttcg cagagattaa tatgaattat
      721 cgggggagta ttatcgccgc tatttttacg gtcaaaaagc ggatcagata ctatagattt
      781 tcgaagataa ccttcagtgt acccctttcg tacgccttct gttatcgcgt ccgatacaag
      841 cagcgtagga cctccagtta ttttaacatc aacaccaatt gttacaaaaa aaactgctgt
      901 tcccgtatcc tgacaaagcg gaagactctg cttgtcggct atttccacat tttgtaaata
      961 tttatctata atgccttgcg atctgccatc ggtttccctc ttaagtgcag tacgataata
     1021 ttgcattgtg cattccggca atctgatatt ggagtttatg caagcatgcg caactgtctc
     1081 cacaatttcg ttgtaatcaa tgtttctcat aacttgactc ccagtttcaa taacgcgact
     1141 ctaaatgacg caagcgaaga agcaagagga atatttttag gacaatagct gtcacaggcc
     1201 aaaagagccg tacatccaaa aactccgtta tcagtcgcgt aaagctcata ccaatcttta
     1261 ttggcccggc catcacgcgg atcactcata aggcgccaag cgttcaacaa ctcggccgct
     1321 ccggcaaaat cagcattcat ctgcacggta gcgcatgcag acacacagca accacactcg
     1381 atacaacgat caagctcgta aagatgcatc ataagttcgt tttcataatt actattagct
     1441 gcgagctcag cctcctgctg ttttgaccta ttcacattaa gcgcctggcg gttatgtatc
     1501 tttataaaat aacttcctgt atctacagac aaatcaccca acataagaaa accaggcaat
     1561 ggagcaatag tcacgctccc ttttacctcg ttcaaacagg ttctacaggc caaacgaggt
     1621 ttaccgttta taagcatagc gcaacttcca caaacacctg accgacatac ataatcaaat
     1681 tttaaagaag gatcaatata tttctgtatc tgttgcaaaa gcgaaagaat agtatcacca
     1741 tctggctcaa catcatagct ttcaaagtac ggtttatttt cactcccagg cttataacgt
     1801 aatattttta ctctcttata catgcaaact gacgtcctca taatctatca taactgattg
     1861 tgtgtcatta ttccaacgag tcaatgtacg tttctggaac tcctctgaca tgtcaggata
     1921 atcgtccctg ttatggcatc cccttgactc cttacgtgcc aatgcaccct ctacaaccgt
     1981 cattgccaaa agaatcatcc ctctgcattc cagtgcattt tcaatttcag gagacatgga
     2041 ataaaagttt ttagaaacca cagcaccatc cactttttca agaatccggc ataactcctt
     2101 tttagctagt ttgagccctt cttcatttcg aaacactccg gcatacctgc tcataaccga
     2161 tcttagatca aatgttaact ctgaaaaagt taccccgtta ctgccggctt tttcttcaag
     2221 ttttacaagg cgatcgcatt gtcctttata ttcttttgta ataacagcgg ctgatggagt
     2281 ataaccatta ttagaaaaat attcacacat gccctctgca gccaattctc cagaaactac
     2341 agtc
//
LOCUS       DAOTGZ010000330         2322 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_3064
            NODE_54714_length_2322_cov_5.266873, whole genome shotgun sequence.
ACCESSION   DAOTGZ010000330 DAOTGZ010000000
VERSION     DAOTGZ010000330.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868088
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 2322)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2322)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:22:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,785
            CDSs (total)                      :: 2,751
            Genes (coding)                    :: 2,732
            CDSs (with protein)               :: 2,732
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 12 of 19
            Pseudo Genes (internal stop)      :: 5 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2322
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_54714_length_2322_cov_5.266873"
                     /isolate="AMR_MDS_3064"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1022
                     /locus_tag="P5123_13655"
     CDS             <1..1022
                     /locus_tag="P5123_13655"
                     /inference="COORDINATES: protein motif:HMM:NF013079.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="M17 family metallopeptidase"
                     /protein_id="HRX17353.1"
                     /translation="FLGETGEYKEKVLNTALVCNNSNLCRDMVNSGTEEVNPDSIEKA
                     ATVIAKIKNMSMRVLNEKKLISKKMNLICAVGRAGRYRPRILELKYKGGKSSDKWIAF
                     VGKGVTFDTGGLDVKPAAGMLDMRYDMAGAASVLYAIKSIAELKLPVNVYAVIPLVEN
                     AVDSQSYKPGDVFVSYSGKSVHVQNTDAEGRLILADAVTYAEKDLKADYIIDMATLTG
                     ACSIALGSEYAALVTYDDKLRDALVSSSLKTGEKLWQLPINKAYEKLIKSDIADIANI
                     SADRKAGTIVGAAFIKQFIDKSRWAHIDIASTAWADSAGAMYGKYSTGYGVRLLTDFA
                     SGSLL"
     gene            complement(1224..1673)
                     /locus_tag="P5123_13660"
     CDS             complement(1224..1673)
                     /locus_tag="P5123_13660"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRX17354.1"
                     /translation="MEQTDFRTSIDVELSRLAKIEEYAAKHNMDISDVINTVLDTYSK
                     KLVRNPVSNDTLVKYQERGTKYESVHIRFSSIAKKRLKDIANLYCYSLSYMLFLAMDF
                     FEDFMNGVKEFVRPCFHSTFVRNSSGFPCFINSWGINFYEDQDHFSG"
     gene            complement(1817..>2322)
                     /locus_tag="P5123_13665"
     CDS             complement(1817..>2322)
                     /locus_tag="P5123_13665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013313587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="sodium:proton antiporter"
                     /protein_id="HRX17355.1"
                     /translation="LMIIMGKHEVEKFFHEVEWETIFFFIGLFILVGGLVELGVIEKM
                     SKFIIALTKNNINHTAYVIIWVSGIFSGIIDNIPFVATMIPLVENIGDTMGANAIKPL
                     WIALSLGSCLGGNGTLIGASANVISAGICEKSGYKISFLDFTKYGAMVTIITLITSTI
                     YINLVFF"
BASE COUNT          704 a          412 c          506 g          700 t
ORIGIN      
        1 cttttttggg tgagaccgga gaatacaaag agaaggttct taatacagca cttgtatgta
       61 acaacagcaa tctatgtcga gacatggtaa attcgggtac cgaagaggtt aatcccgatt
      121 caattgaaaa ggcggcaact gttatcgcaa aaataaaaaa catgtctatg cgtgtattaa
      181 acgagaaaaa gcttatttca aaaaagatga atcttatatg cgcggtaggt cgtgccggac
      241 gatatcgtcc tcgtatttta gaactaaagt acaaaggtgg taaatcatca gacaagtgga
      301 tagcttttgt aggtaagggg gtcacctttg atacaggcgg gcttgatgtg aaaccggctg
      361 ctggcatgtt ggatatgcgt tatgatatgg ctggtgcggc ttcggtgctg tatgcgataa
      421 agtcaattgc agagcttaaa ctgccggtta acgtttatgc tgttattcca cttgtcgaaa
      481 acgctgtcga ttcacagtcg tacaaaccgg gggatgtttt tgtatcatac tcaggaaaaa
      541 gcgttcatgt tcagaacacc gacgcggagg gacgcctgat tctcgctgat gcggtgactt
      601 atgccgaaaa ggatctaaaa gccgattata ttatcgatat ggcaacattg acgggtgcct
      661 gttctatcgc gcttggaagt gagtatgcgg cacttgttac atatgatgat aagttgaggg
      721 atgccctcgt atcctcttcg ttaaaaactg gtgaaaaact gtggcagttg ccaatcaata
      781 aggcttatga aaaactaatc aaatcggata tagcagatat cgcgaatata tcagcggatc
      841 gtaaagccgg aacaattgtt ggtgccgcct ttatcaaaca gtttatcgac aagagtcggt
      901 gggcacatat cgatatagca agcacagcct gggccgatag tgcaggagca atgtatggaa
      961 agtattcgac tggttatggg gtgcgtctgt tgaccgactt tgcgtcaggg tcattattgt
     1021 aattatgtag ggaagtggcg ctggtaattc ggcgtcattt ctttaaaaaa ggcataccga
     1081 tattgatgca tgctgtttgg agagaatgct tttttaaaat tgatagccga attgtctatt
     1141 tttttatttc tgttctgttt aactgattaa taatcagtta aacaggtaat ctaaatcaaa
     1201 aaaaatagga tttttcgggt ttcttagcca gaaaagtggt cttgatcttc gtaaaagttt
     1261 attccccaac tgttgataaa gcagggaaac cctgaagaat tgcgaacaaa agtagaatga
     1321 aaacagggac ggacaaactc tttgacacca ttcatgaaat cctcaaagaa atccattgcc
     1381 agaaatagca tgtacgacaa gctgtaacag taaagattag caatatcctt taatctcttt
     1441 tttgcaatac ttgaaaaccg gatatgcacg gattcgtatt ttgtacctct ttcctggtac
     1501 ttgactagag tatcatttga taccggattc ctgaccagtt tttttgaata cgtatcaaga
     1561 acggtattta ttacatcaga aatgtccata ttgtgctttg ccgcatactc ttcaattttt
     1621 gccagtctgc ttagttctac atcgattgat gtcctgaagt cggtctgttc cataacatgc
     1681 ctccaaagaa ttatctacat atgtatacgt aattggagac tgttttgtta aaattttttt
     1741 attatttatt cataatgagt ttatcctaac cagagatata gtcggtatcg tctctggtta
     1801 gggttgttgg tgtaaatcag aagaaaacaa gatttatata tatcgttgat gtgataagag
     1861 taataattgt taccatggct ccatatttgg taaaatcaag aaaggatatt ttgtagccgc
     1921 ttttttcgca gataccagcg ctaataacgt tagctgatgc tccaatcaat gttccattgc
     1981 cgccaagaca cgaaccaaga gaaagggcta tccataatgg cttaatcgcg ttggcaccca
     2041 ttgtgtcgcc gatgttttcg accaagggaa tcattgtagc tacaaagggg atattgtcga
     2101 taattccaga gaaaatgcca gatacccata taattacata agcagtgtgg ttaatgttgt
     2161 ttttagtcaa agcaataatg aattttgaca ttttttcgat tacaccgagt tcgactagtc
     2221 cgcctacaag aataaagagt ccgataaaga aaaaaatggt ctcccactca acttcatgga
     2281 aaaacttctc aacttcatgt ttgcccataa tgatcattag ta
//
LOCUS       DAOTGZ010000331         2320 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_3064
            NODE_54801_length_2320_cov_2.928477, whole genome shotgun sequence.
ACCESSION   DAOTGZ010000331 DAOTGZ010000000
VERSION     DAOTGZ010000331.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868088
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 2320)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2320)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:22:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,785
            CDSs (total)                      :: 2,751
            Genes (coding)                    :: 2,732
            CDSs (with protein)               :: 2,732
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 12 of 19
            Pseudo Genes (internal stop)      :: 5 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2320
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_54801_length_2320_cov_2.928477"
                     /isolate="AMR_MDS_3064"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..375
                     /locus_tag="P5123_13670"
     CDS             <1..375
                     /locus_tag="P5123_13670"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRX17356.1"
                     /translation="PQSDSELTFYTNTSTGVSFDLAFLGQIYGFADVNFNFNNKYEHN
                     TLNNLGGSTGLITTYRYFWKSHLYGKYSKVFWGDETKRLEYGVEQTISFSRNSSFVIR
                     GKKYEIFKNAFTEYSLQFNIFF"
     gene            complement(379..1263)
                     /locus_tag="P5123_13675"
     CDS             complement(379..1263)
                     /locus_tag="P5123_13675"
                     /inference="COORDINATES: protein motif:HMM:NF024109.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HRX17357.1"
                     /translation="MKDLHITLGDGRKLAFIEIGDPQGVPVFFFHGTPGSRKCLKEDA
                     TEFIQNGIRIIATDRPGMGLSDPQANRSYRGWACDVKELADYLRIERYHVAGGSGGGP
                     HALACGIYTSASVLSVTLISSAAPPDIPRLSKGMSKGNRLGFFAARYLPFLLRMGFKS
                     YAKLVKKNPDALMQSIKKQLSPWDRAVFEKAGDSDVMQKAFIKELNEAFNQNPLYVAE
                     DFLLVARPWNFDYSQLDMPVFLWHGEADTLMPVAPVRQLVQMIPGCDATFIPEAGHLL
                     IESDEVGKQIMEKIVALK"
     gene            complement(1277..1990)
                     /locus_tag="P5123_13680"
     CDS             complement(1277..1990)
                     /locus_tag="P5123_13680"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013700.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M23 family metallopeptidase"
                     /protein_id="HRX17358.1"
                     /translation="MTDKKSLSDCKLFVDFPLRGEWSVVNTLGDKIPSHGTDSLGQRY
                     AYDFMITEQGSKLKFYKSSSIKYRTIGVALADCYCYRKPIYSPVSGKVVTVKDGVKEP
                     KRLQPVLDILKVILRMVKIAVIGLFVSAEKIELHKFIGNYIIIRFDDHYAFFAHLVPG
                     SIRVKEGDQLKVGELIAEVGHSGNSTAPHLHFHIMDTEDLLKAKGVFCGFKKYRIIKD
                     DVEEDVVNGIPKSDQIIKY"
     gene            complement(1987..>2320)
                     /locus_tag="P5123_13685"
     CDS             complement(1987..>2320)
                     /locus_tag="P5123_13685"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRX17359.1"
                     /translation="DALTSDPILKVWSDREQWHKIIDKCRDSSIGLQEEQISKTMFGS
                     IIKNWQEQGIARADMEPDFVLAMFDSLFLAHLYRDDIGAQHFPALIDTMARFITRGIM
                     VSDKRGRS"
BASE COUNT          692 a          533 c          420 g          675 t
ORIGIN      
        1 cctcaatcag atagcgaatt aaccttctat acaaatacat caaccggtgt cagttttgat
       61 ttagcgtttc taggacaaat ttacggattt gctgatgtaa attttaactt taacaataaa
      121 tacgaacaca atacattaaa taatcttggt ggttctacag gtcttataac tacatatcgc
      181 tacttctgga aaagccattt atacggtaaa tattccaaag ttttttgggg agacgaaacc
      241 aaacgattgg aatacggtgt agaacaaaca atatcatttt ctcggaacag ttctttcgtt
      301 attagaggaa aaaaatacga aatctttaaa aatgcattta ccgagtactc tttacaattt
      361 aatatatttt tttaaatctt acttcagagc aacaatcttt tccataattt gtttgccaac
      421 ctcatcgctt tcaataagca gatgcccggc ctctggtata aatgtcgcgt cgcagccggg
      481 tatcatttgc acaagctgcc ttacaggggc taccggcatg agcgtgtcgg cttcaccgtg
      541 ccacaaaaat acaggcatat caagttgaga atagtcaaag ttccagggac gcgctacaag
      601 caaaaagtct tcggccacat agagtggatt ctgattaaag gcttcgttca attctttaat
      661 aaaggctttt tgcataacat ctgaatctcc tgccttttcg aatactgctc tgtcccaagg
      721 ggataactgt tttttaatgg actgcataag ggcatctgga ttttttttaa caagcttagc
      781 atagcttttg aatcccattc ttagcagaaa aggaaggtaa cgcgccgcaa aaaaaccaag
      841 acggttaccc tttgacatac cttttgaaag cctcggaata tcaggcggag ccgcgctgct
      901 tatcaacgtc acggaaagta cactcgcgga agtatagata ccacaagcca aggcatgagg
      961 ccctcctcca gaaccacccg caacatgata gcgctctatc ctcagataat cggcaagctc
     1021 tttaacatca cacgcccagc ccctatagct tctgttagct tgcgggtcgg acaagcccat
     1081 accgggacgg tctgtagcta taatcctgat accgttttga ataaactctg ttgcatcttc
     1141 ttttaggcat ttacgcgaac ccggtgtacc atgaaaaaag aaaacaggca caccttgcgg
     1201 atcaccaatt tcaataaacg cgagcttacg accatcgccc aaagttatat gtaaatcttt
     1261 cattataagc ttcctcttaa tatttgatta tctgatcaga cttggggatg ccgtttacaa
     1321 catcctcttc gacatcatct tttattatac gatacttttt aaaaccgcaa aagactcctt
     1381 ttgcttttaa caaatcctcg gtatccatta tatgaaaatg gagatgcgga gctgtagaat
     1441 taccggagtg accgacctct gctattagct cacccacctt taactgatca ccctctttta
     1501 cccgtattga tcccggaaca agatgcgcaa aaaacgcata gtgatcgtca aacctgatta
     1561 tgatatagtt gccaataaat ttatgaagtt ctattttttc ggcagagaca aagagcccga
     1621 tgacagcaat ctttaccata cgcaaaatta cctttagtat atcaagtacc ggttgcagtc
     1681 tctttggttc tttaacccca tctttgactg tgacaacctt gccgctaaca ggcgaataaa
     1741 tcggtttacg gtaacagtaa caatcggcaa gagcaacacc gattgtacga tattttatac
     1801 tagatgattt ataaaatttt agttttgatc cctgctctgt tatcataaaa tcgtaggcat
     1861 aacgctgccc aagagagtct gttccgtggc tgggtatttt atcccctaga gtattaacaa
     1921 ccgaccactc gccccgtaac ggaaaatcaa caaagagttt gcaatcgctt aatgattttt
     1981 tatcagtcat gatctacctc ttttatcaga gaccataatg ccccttgtta taaatcgcgc
     2041 catggtatcg attaacgccg gaaaatgttg cgcaccaata tcatctcggt atagatgagc
     2101 caaaaaaagc gagtcgaaca ttgccaaaac aaaatcaggt tccatatcag ctcgtgctat
     2161 cccctgctct tgccagtttt tgattataga accgaacatt gttttggaga tctgctcctc
     2221 ttgtaaacca attgatgaat cgcggcactt gtcgataatc ttgtgccact gctccctgtc
     2281 gctccaaacc ttaagaatcg gatcgcttgt taaagcatcc
//
LOCUS       DAOTGZ010000332         2306 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_3064
            NODE_55228_length_2306_cov_6.155486, whole genome shotgun sequence.
ACCESSION   DAOTGZ010000332 DAOTGZ010000000
VERSION     DAOTGZ010000332.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868088
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 2306)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2306)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:22:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,785
            CDSs (total)                      :: 2,751
            Genes (coding)                    :: 2,732
            CDSs (with protein)               :: 2,732
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 12 of 19
            Pseudo Genes (internal stop)      :: 5 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2306
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_55228_length_2306_cov_6.155486"
                     /isolate="AMR_MDS_3064"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1221)
                     /locus_tag="P5123_13690"
     CDS             complement(<1..1221)
                     /locus_tag="P5123_13690"
                     /inference="COORDINATES: protein motif:HMM:NF016330.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SWIM zinc finger family protein"
                     /protein_id="HRX17360.1"
                     /translation="MPITVEIIKELLDPQIYQRSLDYFYTDHVRSVVTDDYPLVTAMV
                     KGNNRNVYRVTIDLESNKYICSCPYPHTCKHIGAVLLELVDIMDQNPKTYSQEQEATI
                     LRNIINERKKILSQMGEKTVNIKPSQAVANNDFLPEATLRSTTAKDRAGKFTFVLLVR
                     YDKYADRYYLRRGLRYIKKDGSPGRIEEYNSKKEIVPIPKECLLINTMLLHKDEIDLS
                     EATDYIAQANDLPLFFNNNKNYFQLETKKISKITIKFRVSYVEFENGSSQIDTEECVI
                     FKPLIIINKQIKQNQGIPIERFFSSARNFGAVSEDNLLHIGINAPLTSYIIQMLKSKK
                     ELDTNDIQKLQAYAQNNSSANIEVVFNHREVNFRFCAPEPVLYIEGDIVNCSFTLHFK
                     YNTREISAGDPASLL"
     gene            1477..>2306
                     /locus_tag="P5123_13695"
     CDS             1477..>2306
                     /locus_tag="P5123_13695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020611619.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal-phosphate dependent enzyme"
                     /protein_id="HRX17361.1"
                     /translation="MNNYFKSMPDQKGFFKEYGGSFIPPDLQTEMDKITDAYYKISKS
                     HEFIAELRDIRKHFQGRPTPVYYCRRLSNELGGRIYLKREDLNHSGAHKLNHCMGEAL
                     LAKYLGKKRLIAETGAGQHGVALATAAAYFGLECEIHMGEVDIAKEHPNVVRMKILGA
                     EIVPVSAGLKTLKEAVDSAFMSYLKDPINTIYCIGSVVGPHPFPMMVRDFQRCVGIEA
                     KEQFFEMTGEMPDNVVACVGGGSNAMGIFSAFLDEEDTHLIGVEPAGRSLKKGDHAAT
                     "
BASE COUNT          665 a          394 c          500 g          747 t
ORIGIN      
        1 cagtagggat gccggatccc cagcactgat ttctcgagta ttgtatttaa agtgtaatgt
       61 aaaagagcaa ttaacgatat ccccctctat atacaaaaca ggctcaggag cacaaaatct
      121 gaagttaacc tccctatggt taaatacaac ttcgatgttt gcggatgaat tgttttgtgc
      181 gtaggcctgc agcttttgta tgtcattggt atccagctct tttttggatt taagcatttg
      241 aattatatat gatgtcaagg gggcatttat gccaatatga agtaagttgt cttccgagac
      301 agcaccgaag tttcgggccg aagaaaaaaa acgttctatg ggaataccct gattttgctt
      361 tatctgttta ttaataataa taaggggctt aaaaataacg cactcttctg tatctatttg
      421 tgatgatcca ttctcgaatt cgacatagct gactctaaat ttgattgtta tcttcgaaat
      481 ttttttagtt tccagctgaa aataattttt gttattgtta aaaaatagag gcaaatcatt
      541 tgcttgtgcg atatagtctg tcgcttcgga aaggtctatt tcatctttat gaagcagcat
      601 tgtatttatt agtaagcact ctttggggat gggcacaatc tcctttttgc tgttatactc
      661 ttcaatacgc cctgggcttc catccttttt tatataacga agacctctgc gaagatagta
      721 cctgtcggca tacttgtcat accgtacaag caaaacaaat gtaaatttcc cagctctatc
      781 tttagctgtt gttgaacgaa gtgttgcttc gggtaaaaag tcgttattag cgactgcttg
      841 agacggtttt atatttacag tcttttcgcc catttgactt agtatttttt tgcgttcatt
      901 aatgatattt ctaagaatgg tagcttcctg ttcttggctg tatgttttag gattctggtc
      961 cattatatca actagttcaa gaagtacagc ccctatatgt ttgcaggtgt gcggatatgg
     1021 acaactgcaa atatatttgt ttgactctaa atctatggtt actcgataca cattgcgatt
     1081 gtttccctta accattgcag taactagtgg atagtcgtca gtaactacag atctgacatg
     1141 gtcggtgtaa aagtaatcta gggatcgttg ataaatttgt ggatcaagca actctttaat
     1201 gatttcgact gttataggca taaatagact ccattttttt taaagttttt atgcttgctt
     1261 gatgtgaaat aatggggttt ccgtttttta agagaagacg gaaggtagtg tcgagaactg
     1321 ggagcaagaa atatataata taaaaacctt tataaaatag caactatttt ttataaacat
     1381 tacaagtaga aataatcaat gggttatgat tctgttgaca gaaacaatca taaatgaaat
     1441 aatatccata atattgcgag cacaaggaga accgctatga ataattattt taaaagtatg
     1501 ccggatcaaa aaggtttttt taaagaatac ggtggaagct ttattcctcc cgaccttcag
     1561 accgaaatgg ataaaattac tgatgcttat tacaaaataa gtaaatctca cgagtttatt
     1621 gcggaattac gtgatatacg caagcatttt caggggcgtc ctactcctgt ttactactgt
     1681 cggcgattat caaatgagct gggtggccgt atttacctga aacgagaaga ccttaatcat
     1741 agcggagcac ataaactgaa tcattgtatg ggagaggcgt tgttggctaa atatcttggc
     1801 aaaaaaagat taattgccga aacaggcgcg gggcagcacg gtgtagcgct tgcgacggcc
     1861 gccgcgtact ttggacttga gtgtgaaata catatgggtg aggtggatat cgctaaagaa
     1921 catccaaatg ttgttcgtat gaagatactt ggcgcggaaa ttgtgccggt aagcgctggg
     1981 cttaagacac tcaaagaggc ggttgattcg gcttttatgt cttatcttaa ggatcctata
     2041 aacaccatat actgtatagg ttctgttgta ggtccccatc cttttccaat gatggttcga
     2101 gattttcaac gatgtgtggg tatcgaggct aaggagcagt tttttgaaat gactggtgaa
     2161 atgccggata atgttgttgc ctgtgtgggc ggtggaagta acgcgatggg aatttttagc
     2221 gcgtttcttg atgaagagga tactcacctt attggagtag agcccgccgg tcgttcacta
     2281 aaaaaaggtg atcacgccgc gaccat
//
LOCUS       DAOTGZ010000333         2289 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_3064
            NODE_55787_length_2289_cov_4.372874, whole genome shotgun sequence.
ACCESSION   DAOTGZ010000333 DAOTGZ010000000
VERSION     DAOTGZ010000333.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868088
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 2289)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2289)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:22:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,785
            CDSs (total)                      :: 2,751
            Genes (coding)                    :: 2,732
            CDSs (with protein)               :: 2,732
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 12 of 19
            Pseudo Genes (internal stop)      :: 5 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2289
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_55787_length_2289_cov_4.372874"
                     /isolate="AMR_MDS_3064"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..699)
                     /locus_tag="P5123_13700"
     CDS             complement(<1..699)
                     /locus_tag="P5123_13700"
                     /inference="COORDINATES: protein motif:HMM:NF012711.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I adenylate-forming enzyme family protein"
                     /protein_id="HRX17362.1"
                     /translation="MSEIKNVNVFSLLESAAKDEPDSVFFNFEGDTVTYKEGFDKTRI
                     CAQHLISRGVKKGDRVILCLQNTPDFVYSYLAISQIGAVSVLVGPVARRFEIKYIVSH
                     SQPSLIITSHTMLDNFTVNNSFFVDTDKFLLVDDSHKSRSLAGIIEAGKPYEKSEAIT
                     DDSAVSIIYTSAMDGFPLGVQLTHKGILASTKVLAGFLNQDDKCISVLPLFHAFGLTV
                     TLIMPIQGRTPFTLV"
     gene            complement(970..>2289)
                     /locus_tag="P5123_13705"
     CDS             complement(970..>2289)
                     /locus_tag="P5123_13705"
                     /inference="COORDINATES: protein motif:HMM:NF012925.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="family 16 glycosylhydrolase"
                     /protein_id="HRX17363.1"
                     /translation="FIDKSKWTFDLGNGFYNDDEYVPGWGNNEKQYYTDSNCYVRYGS
                     LHIKAKEETTKAEINGEKIRFDYTSTRIKTDGLFSKKYGRFEIRAKLPAGKGLWPAIW
                     LLPQNSEYGSWAASGEIDIMEAKGSDFLKVYGTIHYGSEWPSNRFSSESYEFPFADPP
                     KDITQWHVYALEWEPGELRWYVDGNLYQTQNLWSSNDNPYPAPLDKEFYLLMNLAVGG
                     TYDGDPDDSTKFPATMEIDYVRIYELTGRKYREPVKPDPSEYMDPRPANARPALSDGN
                     EVYNNGFSMNKSGVKNISGLKGSSYWYFLHLEAFGGDGEISFENIESTKYAKIEIAEP
                     GNQFYSIQLIQDVPVVKGHTYKASFKAKATDTRKIMVKISGDQTRDWRSYSPVTVIEL
                     TPELKTYSINFTMPEESDLNARYEFNVGLDKSTVWIGEITLTDISPR"
BASE COUNT          650 a          464 c          433 g          742 t
ORIGIN      
        1 taccagtgta aatggagtcc gaccctgtat aggcatgata agcgtaactg ttaatccaaa
       61 agcatggaaa agtggaagca cgctgatgca tttatcgtct tgatttagaa aacccgctag
      121 aaccttggtg gatgcgagta ttcctttgtg agtaagctga acacccagag ggaaaccatc
      181 cattgctgat gtatagataa ttgatacagc tgaatcatca gtaattgctt cggatttttc
      241 atatggtttc ccggcttcga ttattccggc aaggcttcgt gatttatggc tatcgtcaac
      301 tagcagaaat ttatccgtat caacaaaaaa ggagttattt accgtaaaat tatcaagcat
      361 tgtatgagaa gtgatgataa gcgatggttg cgaatgtgat acaatgtact tgatttcaaa
      421 ccttcttgca acaggaccaa ccagaacact aaccgcaccg atttgcgaaa tcgcaagata
      481 gctgtataca aaatcaggtg tattctgaag acataaaatt accctgtcgc cttttttgac
      541 tccacgtgat attagatgct gcgcacaaat ccttgtcttg tcaaaaccct ctttataggt
      601 cactgtgtct ccctcaaaat tgaaaaagac cgaatctggc tcatccttgg cggcagactc
      661 tagaagcgaa aaaacattta cattctttat ttcagacatt tttatcttcc tattatattt
      721 cggaatcatg ttttataata aacaaaacca aagaatgaat attataagat tatttccgat
      781 taagttacta caagagtaat taaatgataa caattattaa aaagtcatca atcaattgca
      841 cttaattaat agaaattaac cctattaaga gtcaagtaat ttataaactt gtgaaaaatc
      901 gatattcgtg ttatttgtat gtcaaaagtt accgaccgct tccctttagc aggtcacggc
      961 cggctgaact taacggggac ttatatcggt aagagtaatt tctccaatcc atacagttga
     1021 tttatccagg ccaacattga attcgtatct tgcgttaagg tcgctttctt cgggcattgt
     1081 gaaatttatg ctgtaagtct tcaattcggg tgtcagctca attaccgtga ccggcgaata
     1141 tgatctccag tctcgtgtct ggtcaccact gattttaacc ataatttttc ttgtatctgt
     1201 tgcttttgcc ttgaaactgg ctttgtaggt gtgacctttg actactggaa catcctgtat
     1261 aagttgaata gaataaaatt ggttgcctgg ttcagctatc tcaatttttg cgtattttgt
     1321 tgattcaata ttttcaaagc ttatttctcc atcgcctccg aatgcttcca gatgaagaaa
     1381 ataccagtag gagcttcctt tcagtcctga gatatttttt acaccggatt tgttcatgct
     1441 aaatccatta ttatatactt cattgccgtc actcagagcc ggtctggcat tcgcggggcg
     1501 tggatccata tattcgctcg gatcgggttt tacgggttct cgatacttac gtccggttaa
     1561 ttcgtatatt cttacataat cgatctccat tgttgctgga aatttggtgg aatcgtcagg
     1621 atctccatcg taagtaccac caactgcaag gttcataaga agataaaact ccttgtctaa
     1681 tggagcagga taggggttat cattggagct ccataaattc tgtgtttgat aaagattgcc
     1741 atctacatac cagcgtagtt caccgggttc ccactcaaga gcataaacat gccattgggt
     1801 aatgtctttt ggaggatcgg caaatggaaa ttcatacgac tcactactga agcggtttga
     1861 gggccattcc gacccatagt gaatagtgcc atatactttt aaaaagtcac ttcctttagc
     1921 ttccattata tcaatctctc cggaagctgc ccatgaaccg tattcgctgt tttgtggaag
     1981 caaccatatt gcaggccaaa gacctttgcc agcaggaagt ttggctcgta tttcaaagcg
     2041 tccgtatttt ttactgaaaa ggccatctgt cttgatacgt gtagaggtat agtcaaaacg
     2101 tattttctct ccgtttattt cggcctttgt tgtttcttct ttggccttaa tatgcaaaga
     2161 gccatatctt acgtaacagt ttgagtccgt atagtactgt ttttcattgt ttccccagcc
     2221 cggtacatac tcatcatcat tgtaaaatcc gttccctaaa tcaaatgtcc attttgattt
     2281 atcaataaa
//
LOCUS       DAOTGZ010000334         2263 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_3064
            NODE_56664_length_2263_cov_3.909420, whole genome shotgun sequence.
ACCESSION   DAOTGZ010000334 DAOTGZ010000000
VERSION     DAOTGZ010000334.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868088
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 2263)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2263)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:22:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,785
            CDSs (total)                      :: 2,751
            Genes (coding)                    :: 2,732
            CDSs (with protein)               :: 2,732
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 12 of 19
            Pseudo Genes (internal stop)      :: 5 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2263
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_56664_length_2263_cov_3.909420"
                     /isolate="AMR_MDS_3064"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..377
                     /locus_tag="P5123_13710"
     CDS             <1..377
                     /locus_tag="P5123_13710"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRX17364.1"
                     /translation="TMVMVGGFVGQIMAMDPKLTISQAYAAGLVHSSSTMVGGFIGFY
                     ADGVFENCYWDTVETGRNNVAGNGSPTGITGLTDIEVHTESKYVGWNFTTPPADWIMV
                     SDVNDNYPYPMSFYNLWGSDVP"
     gene            832..1347
                     /gene="def"
                     /locus_tag="P5123_13715"
     CDS             832..1347
                     /gene="def"
                     /locus_tag="P5123_13715"
                     /EC_number="3.5.1.88"
                     /inference="COORDINATES: protein motif:HMM:NF001159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042586 - peptide deformylase activity
                     [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide deformylase"
                     /protein_id="HRX17365.1"
                     /translation="MKHHLVYYGHEKLRQNAEPVTEFDSDLVSLSEAMFKIMKKYNGI
                     GLAAPQIALSKKFVTIDLTSYDKEKLVIVNPEIVWRSEKKGPYDEGCLSIPGIFETVD
                     RPIEVVVNAYNLDGKQIEIKADDILARVLQHEIDHLNGIMFVDHLEEYIRKEYTKELK
                     KIKKMNKQALF"
     gene            1408..>2263
                     /gene="fmt"
                     /locus_tag="P5123_13720"
     CDS             1408..>2263
                     /gene="fmt"
                     /locus_tag="P5123_13720"
                     /EC_number="2.1.2.9"
                     /inference="COORDINATES: protein motif:HMM:TIGR00460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004479 - methionyl-tRNA formyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006431 - methionyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionyl-tRNA formyltransferase"
                     /protein_id="HRX17366.1"
                     /translation="MKIGFFGTPEVARGLLAHLCSNYDVCFVVTNCDKPAGRNKKVCK
                     PPVKMCAEENNVEILQPETLKDDVLVEHLKSFNADLFIVFAYGHLIPASVFEMPRYGT
                     INLHPSKLPVYRGAAPVEWALMDGISETAVSIQKITEKLDAGDILAQQNITIDPDMNA
                     KELYEKVMPVGISLVDDVIKGFEEETLNPVKQDESQATYCKKISRSTARIDWNMKALQ
                     IHNLVRAMNPKPGAWSLFRDTEIKVWQTAIVPQDQMIDLKPGQLARLGKKRLVAGCGD
                     GIIEIISIQ"
BASE COUNT          702 a          370 c          503 g          688 t
ORIGIN      
        1 tcacaatggt aatggtgggc ggttttgtag ggcaaattat ggctatggat cccaagttga
       61 caatatcgca agcctatgct gcaggactgg ttcatagcag tagtactatg gtgggtggat
      121 tcattggttt ttatgccgat ggcgtatttg aaaattgcta ctgggatacg gtggagactg
      181 gacggaacaa tgttgcgggt aatggaagtc cgaccggtat tactggttta actgacattg
      241 aagtacacac tgaaagtaaa tatgttggtt ggaattttac gacccctcct gcagattgga
      301 taatggtttc agatgtgaat gacaactatc catatccgat gagcttttat aatctgtggg
      361 gcagtgatgt accctaatga gtagtttgtg atagaatatt atatttaagt atggaacccc
      421 caaatgtttt ttgggggttt gtttttggac aaatccggtt tcttggtaag agagagacat
      481 atctcgattc cagaactgat tacttggttt gttatccagt ttaagcatca tttaaatcgt
      541 tttattgtat tgttagccag ctttccggat ctttgcatgg taaaagtgaa ttgttagaaa
      601 gcataattga atcagaatga tttttcttcg gttttttttc tgaaaaatgg attattaaac
      661 agcatcatta tcaaattgac gaccatcaat caacctcatg agtcaaaatc ttcagctata
      721 taattgcgat tatttctttt ctaaagtgtt gctcaatatg tattcgaaga aaatttattg
      781 aaattaaaga cgaatataat caactggtta agtagttgtt tggaggttaa tatgaaacat
      841 catcttgtgt attacggtca cgaaaagctt aggcaaaatg cggaacctgt taccgaattt
      901 gattcagatc ttgtatcatt gtcagaagca atgtttaaaa taatgaaaaa atataacggt
      961 ataggtctgg cggcgcccca gattgcgctt tcaaaaaaat ttgttacaat tgatcttacc
     1021 tcttatgata aagaaaaact tgttatcgtg aatcctgaga ttgtatggag atcagaaaag
     1081 aaagggcctt atgatgaagg atgcctttct attccgggaa tttttgaaac cgtagaccgc
     1141 ccaatagaag tcgttgttaa cgcgtataat ctagatggca agcagatcga gatcaaggcc
     1201 gatgacatac tagcgagggt attacaacac gaaattgatc atcttaatgg tataatgttt
     1261 gttgatcatc ttgaggagta tatccgaaag gagtatacga aggagctgaa aaaaataaaa
     1321 aaaatgaata aacaggctct cttctaatat ttattgtagt tttcaatgtc agatgatttt
     1381 tattattagt gggttgaggt cttaaatatg aaaatcggct tttttggtac acctgaagtt
     1441 gctcgtggtt tgctggcgca tctttgctct aactatgatg tctgttttgt agttaccaat
     1501 tgcgacaaac cggcgggacg aaacaaaaag gtttgcaagc caccggtaaa aatgtgtgcc
     1561 gaagagaaca atgtcgagat acttcaaccg gaaaccctta aagacgatgt tctggtagag
     1621 catcttaaaa gttttaatgc cgacttattt atagtattcg cttatggtca tcttataccg
     1681 gcttccgttt tcgagatgcc tcgctatgga accatcaatc ttcatccttc aaaactacct
     1741 gtatacaggg gagccgctcc ggtagagtgg gcattgatgg atggaatttc tgaaactgct
     1801 gtctccatac aaaagataac cgaaaaactc gatgcaggtg atatacttgc tcaacagaat
     1861 attaccattg atcctgatat gaatgccaaa gagctttacg aaaaggttat gcctgtaggt
     1921 atatcattag tagacgatgt gataaaagga tttgaagaag aaacattaaa tccggtaaaa
     1981 caggatgaat ctcaggccac ctattgcaag aagatatcac gaagtacggc acgtattgac
     2041 tggaatatga aagcgttaca aatacataat cttgtccgag caatgaatcc caagccaggt
     2101 gcgtggtctc tattccgtga tacagaaata aaggtgtggc aaactgcgat agttccacag
     2161 gatcagatga ttgatcttaa gccgggacag ttagccaggc tgggcaaaaa aagacttgtt
     2221 gccggatgcg gtgatggaat aatcgagatt atctctattc agc
//
LOCUS       DAOTGZ010000335         2182 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_3064
            NODE_59576_length_2182_cov_4.876352, whole genome shotgun sequence.
ACCESSION   DAOTGZ010000335 DAOTGZ010000000
VERSION     DAOTGZ010000335.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868088
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 2182)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2182)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:22:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,785
            CDSs (total)                      :: 2,751
            Genes (coding)                    :: 2,732
            CDSs (with protein)               :: 2,732
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 12 of 19
            Pseudo Genes (internal stop)      :: 5 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2182
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_59576_length_2182_cov_4.876352"
                     /isolate="AMR_MDS_3064"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..339)
                     /gene="grpE"
                     /locus_tag="P5123_13725"
     CDS             complement(<1..339)
                     /gene="grpE"
                     /locus_tag="P5123_13725"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0000774 - adenyl-nucleotide exchange
                     factor activity [Evidence IEA];
                     GO_function: GO:0042803 - protein homodimerization
                     activity [Evidence IEA];
                     GO_function: GO:0051087 - chaperone binding [Evidence
                     IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide exchange factor GrpE"
                     /protein_id="HRX17367.1"
                     /translation="MAEMREKTAEEKKISEQDPVEKSSDKGFPEKEDASVQNGDSGLN
                     EDSSDKESDDEVKNDASSDSKDEEITALKDLMMRRQADFENYKKRCIKQEETNKRMLI
                     RDIALDIIGIN"
     gene            complement(352..1404)
                     /gene="hrcA"
                     /locus_tag="P5123_13730"
     CDS             complement(352..1404)
                     /gene="hrcA"
                     /locus_tag="P5123_13730"
                     /inference="COORDINATES: protein motif:HMM:TIGR00331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_process: GO:0009408 - response to heat [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heat-inducible transcriptional repressor HrcA"
                     /protein_id="HRX17368.1"
                     /translation="MENNGQHRNLTEREAAVLGALVFEYISTGKPVGSRSFVQKYSFN
                     ISPATMRNIMFDLESMEYLAQPHTSAGRVPTDKGYRYYVDRLVGSYELKNADFSGIRS
                     DMLTREIELNKMFESVTRMLSDVSKYAGVILTPKPDFTVVKHIELVPLGHGNLLFILV
                     TRTGIVLNKRVTVSQTLNQDELHRFSKYLTSELNGYSIFSIKDSVLGELRSGLNPNNE
                     MQLAIDIVELALTEDESQELYIEGLENILKIPEMVDESLLLPFLHLIEDRKNLIVILK
                     RNMRSDGVSTLIGEEIDQASITGCSLVTSTYKIGNNHVGVVGIIGPTRMNYKTVVPLV
                     DYTGKVVTELLTKMSK"
     gene            1852..2025
                     /locus_tag="P5123_13735"
     CDS             1852..2025
                     /locus_tag="P5123_13735"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRX17369.1"
                     /translation="MEIKDRELLRQLESIDKLNDEDKDIVKQVLNLIIMKNGFQSLLK
                     HSLSSKEPKVHIL"
     gene            2022..>2182
                     /locus_tag="P5123_13740"
     CDS             2022..>2182
                     /locus_tag="P5123_13740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013032310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system HicB family
                     antitoxin"
                     /protein_id="HRX17370.1"
                     /translation="MNRSFTLEYWIDDDFYIGKIKEVPGVFSQGETLAELEENIKDAY
                     HLMLEEETIP"
BASE COUNT          672 a          431 c          374 g          705 t
ORIGIN      
        1 atttattcct ataatatcaa gggctatatc ccttataagc atacgcttgt ttgtctcttc
       61 ctgtttgata caccgttttt tataattctc aaaatcggcc tgacgcctca tcataagatc
      121 ctttagcgcc gtgatttctt catcttttga gtcagatgat gcatcatttt tcacttcatc
      181 atctgattct ttatcagatg aatcttcatt taaaccgcta tctccatttt gaacagaagc
      241 atcctctttt tctggaaaac ctttatcaga gcttttctct acaggatcct gttcagaaat
      301 ctttttttct tcagccgttt tttctctcat ttcagccatg atttctcctt gctatttcga
      361 catttttgtt aataattctg ttaccacttt gccggtgtaa tcaacaagtg gcacaacagt
      421 tttatagttc atacgggtcg gcccaataat accaactaca cctacatgat tattgccaat
      481 cttgtaagta gaggtaacaa gactgcaccc ggttatactt gcctgatcga tctcttcacc
      541 tataagtgtg gaaacaccat ctgaacgcat attacgttta agaatgacaa taagattttt
      601 acggtcttct atcagatgta aaaaaggcaa tagaagtgac tcatcaacca tttctggtat
      661 ttttagtata ttttcaagac cttcaatata aagctcctgg ctttcgtctt ctgtcaaagc
      721 aagctctaca atatctattg caagttgcat ttcattattt ggattaaggc cggagcgtaa
      781 ttcgcctaaa accgaatctt ttattgaaaa tatcgaatac ccgtttagct ctgacgttaa
      841 gtattttgaa aatctgtgga gctcatcctg atttaaagtc tgagaaacag taacacgttt
      901 gttaagtact ataccagttc tggttactaa aataaacaga agattaccgt gcccaagcgg
      961 aacaagctca atatgcttaa caactgtgaa atccggtttc ggagtcagaa ttaccccagc
     1021 atattttgat acatctgaca acattctagt tacagactcg aacatcttgt taagttctat
     1081 ctcacgcgtt agcatgtcag aacgaatgcc cgaaaaatcg gcatttttaa gttcataaga
     1141 gccgaccaga cgatcaacat aatagcgata gcccttatcc gttggaacac gccctgccga
     1201 tgtatgaggc tgtgctagat actccataga ttcaagatca aacataatat ttcgcattgt
     1261 tgcaggcgag atattaaaag aatacttttg aacaaaagac cgtgagccaa caggttttcc
     1321 ggtagagata tattcgaaaa ccagagcccc tagcacagca gcttcgcgtt ctgtaagatt
     1381 tctatgttga ccgttgtttt ccataatcac cactaagtta gcactcaaat atatagagtg
     1441 ctaataaaat agaaaaaatg gctctattcg tcaagtgaat tatttgagtt tttgcctttt
     1501 tttggatatt ttttttatgt tttgtattta tttgatcttc gtcgcgacgt tctgttgcag
     1561 aaagcattca tgtaattcga caagctctga taattgactt ttcaactaga cacgtatact
     1621 gaacgacctg aaagaaactt tcccacacga cctttcttgt ttctggagtt tttatttcga
     1681 ggcatgaaaa gcctaccggc cgcataaaat gatttgaaaa tcgcatacaa agagggagct
     1741 cccaaggtgt tgagtctgat agtcttttta gaatattaat gatacttaag ccatgactgt
     1801 tggcacagtt gaggctttat ttttttctga gcaaaaagaa gatccgcacg agtggaaata
     1861 aaagacagag aactgttgcg acaacttgag tctatcgaca agttaaacga cgaagataaa
     1921 gacattgtaa aacaagtact caatcttatt attatgaaaa atggctttca atcacttttg
     1981 aaacactcac tctcttcaaa agaaccaaag gtgcatatat tatgaacaga tcttttactc
     2041 ttgaatactg gattgatgat gatttttata ttggaaaaat aaaagaagta ccaggtgtat
     2101 tcagtcaggg cgaaacgtta gcagaacttg aagaaaacat aaaagacgct tatcacttaa
     2161 tgcttgaaga agaaactatt cc
//
LOCUS       DAOTGZ010000336         2158 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_3064
            NODE_60470_length_2158_cov_3.508797, whole genome shotgun sequence.
ACCESSION   DAOTGZ010000336 DAOTGZ010000000
VERSION     DAOTGZ010000336.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868088
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 2158)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2158)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:22:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,785
            CDSs (total)                      :: 2,751
            Genes (coding)                    :: 2,732
            CDSs (with protein)               :: 2,732
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 12 of 19
            Pseudo Genes (internal stop)      :: 5 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2158
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_60470_length_2158_cov_3.508797"
                     /isolate="AMR_MDS_3064"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(150..1328)
                     /locus_tag="P5123_13745"
     CDS             complement(150..1328)
                     /locus_tag="P5123_13745"
                     /inference="COORDINATES: protein motif:HMM:NF014069.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HRX17371.1"
                     /translation="MKTESENYLKNILFDKESNLSKFACLSKEAVDLIPNSDKASPYR
                     TSFGRDIHTIMQTNTYARYMDKTQVFYLFSNQHITHRSLHVQFVSTIARTIGRFLKLN
                     EDLIEAISLGHDLGHPAFGHEGESILNSIALENGVGAFAHNAQSVRTLLNLENNGLGV
                     PVSLQVADGILAHNGEMLQQQYYPCLNKTTDQFKKEFDLCMKDVSYSKTIRPMTMEGC
                     VVRIADVIAYIGRDIEDAIKLKMIKHSSIPREISKVLGQNNNQITETLISDLISCSYG
                     KEYLGFSREIFSALCKLKEFNYKTIYFNPLIKSESKKIDRMFHLLFDLYFDKLKREEE
                     SSDIFIYFLKDRKSDYSKTTDPARIVIDYMASMTDSFIVNQFNKLFFPKNFGYSILSD
                     "
     gene            1621..>2158
                     /locus_tag="P5123_13750"
     CDS             1621..>2158
                     /locus_tag="P5123_13750"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRX17372.1"
                     /translation="MFSAFCKLLSLLSASDKRKLAILIIVILITAFISIAGIASIMPF
                     VAIMGDTSLIMSNEYLNKLYSIFGSPPLHSFVIYLGMAVLILFLAGNLLLTLSVFVKF
                     KVARTISYNLTKAIFTRYLYFPYEFFLARNSSELVKNVFSETETVTNDVITPMLEFIK
                     SFMMSTAIITVLLIMSPGP"
BASE COUNT          658 a          408 c          362 g          730 t
ORIGIN      
        1 tgtaaaccat attaacgaag gatccggcac gcattttgat ccattacttg tggatatttt
       61 taaccgtgaa attgacacca tactggacat caagaaaagc tacccagaac tgactgccga
      121 gacggaatac tgactgcaaa acaaagagat taatcggaca aaatggaata accgaaattt
      181 ttaggaaaaa acagtttgtt aaactggttg actatgaaac tgtctgtcat tgatgccata
      241 taatcaataa ctattcttgc cggatcagtg gttttactgt aatcactttt acggtctttt
      301 aaaaaataga taaaaatatc tgacgactct tcttctctct tcagtttatc gaaataaaga
      361 tcaaaaagaa ggtgaaacat ccggtctatt tttttacttt cactctttat caacggattg
      421 aagtaaatgg ttttgtaatt aaattctttt aacttgcata aagcggaaaa tatttccctg
      481 ctgaaaccca gatactcttt accgtaactg catgatatca gatcgcttat cagggtttcg
      541 gtaatctgat tattgttttg ccccagaacc ttgcttatct cacggggtat tgatgagtgt
      601 tttatcatct tgagttttat cgcgtcctca atatcccgcc caatgtacgc gataacatct
      661 gctattctta ccacacaacc ctccattgtc atgggacgaa tggtttttga ataactcaca
      721 tctttcatac acagatcaaa ctcttttttg aactgatccg ttgttttgtt taaacatgga
      781 taatactgct gctgcagcat ttcaccgtta tgagcaagta taccgtcagc aacctgaagt
      841 gaaaccggaa caccaagacc gttattttcc agattaagaa gtgtccggac actctgagcg
      901 ttatgagcaa aagctccaac accgttttca agggcaatcg aattcaatat agattcgcct
      961 tcatggccaa aggctggatg ccccaaatca tgtccaagag atatagcctc tatcaggtct
     1021 tcattaagtt tcagaaagcg ccctatggtt cgcgctatcg tagagacaaa ctgcacatgc
     1081 agggatcggt gcgtaatatg ctgatttgaa aaaagataaa aaacctgggt cttatccata
     1141 tatcgggcgt aggtatttgt ctgcataatt gtgtgaatat cacgaccaaa tgaagttcga
     1201 taaggagagg ctttatcaga gttcggaatc aaatcaactg cttctttgct taagcatgcg
     1261 aatttggaga gattactctc tttatcaaat aatatatttt ttaaataatt ctctgactct
     1321 gttttcatat tatatgcacc catattgatt atatataatt ttgcacctat atatttcatt
     1381 ccattgtttt aaaaaacaac taataaaccc gaaaaagagt gtttgacaat atgccctacg
     1441 tttattttac tgcatattga tattttttaa aagaaaccac aatcaggaac ttaccaagta
     1501 tcatatttga tacaagaaaa ggattgtctc cttaatttag aaatttttgt atttttcaaa
     1561 gtttcctatt gaagtttcct attgtatttt ccctgtgtaa tagatactgt tggtgatatt
     1621 atgtttagtg cgttttgtaa attattatct cttttatcgg caagcgacaa aaggaagctc
     1681 gctattttga taattgttat acttataaca gcttttataa gtatagccgg tattgcgtcc
     1741 ataatgcctt ttgttgcaat tatgggtgat acttcactaa taatgtcaaa cgaatatctc
     1801 aataaactgt actcaatatt cggcagccct cctctgcata gttttgtaat ttacttggga
     1861 atggcagtat tgattctttt tcttgccgga aatctgctgt taaccttgtc ggtctttgta
     1921 aaattcaagg tcgcacgaac tatctcttat aaccttacaa aagccatttt tactagatat
     1981 ttatatttcc cttatgaatt ttttcttgca cgcaactctt ccgaacttgt aaaaaatgtt
     2041 ttttctgaga cagaaactgt tacaaatgat gtcattacac ccatgcttga atttatcaaa
     2101 agcttcatga tgtcaactgc gataataact gttctgctta ttatgagtcc gggaccag
//
LOCUS       DAOTGZ010000337         2157 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_3064
            NODE_60509_length_2157_cov_4.202188, whole genome shotgun sequence.
ACCESSION   DAOTGZ010000337 DAOTGZ010000000
VERSION     DAOTGZ010000337.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868088
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 2157)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2157)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:22:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,785
            CDSs (total)                      :: 2,751
            Genes (coding)                    :: 2,732
            CDSs (with protein)               :: 2,732
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 12 of 19
            Pseudo Genes (internal stop)      :: 5 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2157
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_60509_length_2157_cov_4.202188"
                     /isolate="AMR_MDS_3064"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..372
                     /locus_tag="P5123_13755"
     CDS             <1..372
                     /locus_tag="P5123_13755"
                     /inference="COORDINATES: protein motif:HMM:NF020133.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRX17373.1"
                     /translation="VYKRLKLSLWVVIVFNDFHICTKEAYKKVLSYNNHYDSSNKIKE
                     LIYLLDFGYYYNSFENYAIEKYPEIACVKKFMYDYGAIFSLMTGSGSSVVGYFKSKQT
                     ACKAAGFMNEKGFDSVVSYLI"
     gene            375..446
                     /locus_tag="P5123_13760"
     tRNA            375..446
                     /locus_tag="P5123_13760"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:407..409,aa:Gln,seq:ttg)
     gene            461..1183
                     /locus_tag="P5123_13765"
     CDS             461..1183
                     /locus_tag="P5123_13765"
                     /inference="COORDINATES: protein motif:HMM:NF012695.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NTP transferase domain-containing protein"
                     /protein_id="HRX17374.1"
                     /translation="MRAIVLAAGKGVRMNSSLPKVLHRINNKPLVQHVIDSLNEVNVE
                     LVCVVVGYRADDVQNEIGDSVSYAFQKEQLGTGHAVLQAESYFKDYSGLIIITCGDAP
                     FVTAQSFKKLISVCSEEKCKAAVLTMICDDPYGYGRIIKDENDNVIGIVEEKDASDSI
                     KQINEVNCGTYVVDSSLLFEGLKSVGTDNAQNEYYLPDIVKYITSKGYIVKSSVLSDN
                     REGIGINSQKELEQAEKIYNEL"
     gene            1173..2132
                     /locus_tag="P5123_13770"
     CDS             1173..2132
                     /locus_tag="P5123_13770"
                     /EC_number="2.7.6.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014262617.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004749 - ribose phosphate diphosphokinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0006015 - 5-phosphoribose 1-diphosphate
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0009156 - ribonucleoside monophosphate
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0009165 - nucleotide biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-phosphate pyrophosphokinase"
                     /protein_id="HRX17375.1"
                     /translation="MNFDITKKKHDIKIISCNSNLTLATDITDKLGVSLTESDCKKFL
                     DGEIFVKIKESVRGCDVFIVQSTCKPANDNLMELLLMIDAAKRASARTITAVIPYFGY
                     SRQDRKMEPRVPISAKLVANLLMKAGIQRVLTMDLHADQIQGFFDVPVDNLFLTPIAI
                     GYFNSLKIDDLVVVSPDSGGTDRARYLAKHLQCGLAIIDKRRPKANVCEIMNVIGEVE
                     GKNCLLIDDIIDTAGSISGGAKALKDFGAKDIYCIATHPVLSGNAVSNLKGAGFKEII
                     FSDTIPIPEDKMLDNMTILRTCSLFSEAIKRIYSGESVSNLFV"
BASE COUNT          694 a          305 c          463 g          695 t
ORIGIN      
        1 gtctataaaa gattgaagtt aagtttatgg gttgtaattg tttttaatga ttttcatatt
       61 tgtactaaag aagcttataa aaaggtatta agttataata accattatga tagcagcaac
      121 aaaattaaag agttgatata tttattggat tttggttatt attataatag ttttgagaat
      181 tatgctatcg aaaagtatcc cgagatagca tgtgtaaaga agttcatgta tgattatgga
      241 gccatttttt cgttgatgac cggttccggt tcgtctgtgg ttgggtattt taaatcaaag
      301 cagacggctt gcaaagcggc aggttttatg aacgagaaag gatttgattc tgtagtatca
      361 tatttgatat aaattgaggc gtagccaagc ggtaaggcac cgggttttgg tcccggcatt
      421 ccaaggttcg aatccttgcg tctcagagag aaggtaaagt atgagagcga ttgttttagc
      481 ggcaggaaag ggtgttcgga tgaattcctc tttacctaag gtgttacacc gtattaataa
      541 taaacctctt gtacaacatg taattgattc actgaacgaa gttaatgttg aacttgtctg
      601 tgtggttgta ggttatagag cagatgatgt tcaaaacgag attggtgatt ctgtatctta
      661 tgcctttcaa aaagagcagc tgggaaccgg tcatgctgta ttacaagcgg aatcctattt
      721 taaagattat agcggactga ttataataac ttgcggtgat gcgccgtttg ttacagccca
      781 aagttttaaa aagctgattt ctgtttgtag tgaagaaaaa tgtaaagctg ctgttttgac
      841 gatgatatgt gatgatccat atggttatgg acggataatt aaagatgaga acgataatgt
      901 tatcggaatt gttgaagaaa aagacgcctc tgactctata aaacagataa atgaagtaaa
      961 ttgcggtaca tatgtagttg actcctccct gctgtttgaa ggattaaaat ctgttggaac
     1021 agataatgcc caaaatgagt attatttacc ggacatagtt aaatatataa cttctaaagg
     1081 gtatattgtt aaatcctcag ttctttcgga caaccgcgaa ggcattggaa taaatagtca
     1141 aaaagaactg gaacaggcag agaaaatata taatgaactt tgatattaca aaaaagaaac
     1201 atgatataaa aattataagt tgcaactcca atttaaccct ggctaccgat ataactgaca
     1261 aattgggtgt ttctttaact gaatctgatt gtaaaaaatt tcttgatgga gaaatttttg
     1321 taaagataaa agagagtgtc cgcggatgtg atgtttttat tgtccagtcg acttgtaagc
     1381 ctgccaatga taatttgatg gagctcctgc tgatgattga tgctgcaaag cgagcgtcag
     1441 cccgtacaat aacagctgtc attccctatt tcggttattc acgacaagat agaaaaatgg
     1501 agccgcgggt tccaatttct gctaaacttg ttgccaattt attaatgaaa gccggaattc
     1561 aacgtgtact gacaatggat cttcacgcag atcagattca gggttttttt gatgttccgg
     1621 tcgataatct gtttttaaca ccaatagcaa taggatattt taattcttta aaaattgatg
     1681 acttagtggt agtatctcct gatagcggtg gaaccgatcg tgcacgttat cttgcaaaac
     1741 atttacaatg cggtttggcc attattgata aacgtcgtcc caaagcaaat gtttgtgaaa
     1801 taatgaatgt aattggagaa gtagagggca aaaattgtct tcttatagat gatattatag
     1861 acactgccgg atcaatttct ggtggagcca aagctttaaa agattttggt gccaaggata
     1921 tatattgtat agctactcat ccggttttat cgggaaatgc agtttctaat cttaagggtg
     1981 ctggttttaa agaaataata ttttctgata caataccgat acctgaagat aaaatgttgg
     2041 ataatatgac gattctacga acatgttctc tcttttcaga agcgattaag agaatatatt
     2101 ctggagaatc tgtaagtaat cttttcgtat aataatattt tagtgaggaa aaaatgg
//
LOCUS       DAOTGZ010000338         2152 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_3064
            NODE_60686_length_2152_cov_4.238436, whole genome shotgun sequence.
ACCESSION   DAOTGZ010000338 DAOTGZ010000000
VERSION     DAOTGZ010000338.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868088
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 2152)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2152)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:22:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,785
            CDSs (total)                      :: 2,751
            Genes (coding)                    :: 2,732
            CDSs (with protein)               :: 2,732
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 12 of 19
            Pseudo Genes (internal stop)      :: 5 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2152
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_60686_length_2152_cov_4.238436"
                     /isolate="AMR_MDS_3064"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..979)
                     /gene="alr"
                     /locus_tag="P5123_13775"
     CDS             complement(<1..979)
                     /gene="alr"
                     /locus_tag="P5123_13775"
                     /EC_number="5.1.1.1"
                     /inference="COORDINATES: protein motif:HMM:TIGR00492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008784 - alanine racemase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alanine racemase"
                     /protein_id="HRX17376.1"
                     /translation="MNYRGTWLEIDTEAIKNNLETIHNITSNRPILLPVKADAYGHGM
                     IETARFAEKSGLINMFGVASIEEGICLRENGINLPILILSYIIPGDEQINALIKYNLM
                     PTIGDIDTAQRCNLVAGAHNIKIKIHIKTDTGMGRLGCPASESVHTCNIINQLENIFI
                     EGFYTHMPVSDESSNPFNNNQIADYKKITEAVRETLGRKPVLHMANSGAVLYYPDTFF
                     DMVRPGIISYGYMPDPASPHDKRFKQSMSLFSTIIMMKKVNAGSALSYGHTYKLNKES
                     WIATIPCGYGDGFHRSLSNNALVVINDRKYPVAGRICMDLLLIDCGDDYF"
     gene            complement(984..1631)
                     /locus_tag="P5123_13780"
     CDS             complement(984..1631)
                     /locus_tag="P5123_13780"
                     /EC_number="2.7.6.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR01378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004788 - thiamine diphosphokinase
                     activity [Evidence IEA];
                     GO_process: GO:0006772 - thiamine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine diphosphokinase"
                     /protein_id="HRX17377.1"
                     /translation="MKKALILCNGRITDYTVLKQALASLMSENPTVIAVDGGAGHAIN
                     LGYKIDTALGDFDSIEPEHMEHITENDIEVISFPADKDMTDSELALHHCIQNGFKNLT
                     VAGFSGTRLDHTIGNIMLLAKLGRDIKIRLVDENNEAFFCTDIIELEAHAGAYISIIP
                     LFGDIVVKNTEGLHYKLSDKRVTPGSTLCISNFAVDRKQIIELAEGQALITISRD"
     gene            1880..>2152
                     /locus_tag="P5123_13785"
     CDS             1880..>2152
                     /locus_tag="P5123_13785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020815358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine decarboxylase"
                     /protein_id="HRX17378.1"
                     /translation="MIKPELLDRWKIKNSEDLYGIKTWGASYFEINDKGEVSVTPFAD
                     KPDVSVSLVDIIKGIKERGLDAPVLLRFENLLDSQLTFLNESFNAAI"
BASE COUNT          597 a          395 c          470 g          690 t
ORIGIN      
        1 caaaataatc atctccgcaa tctataagaa gaagatccat gcaaatacgc cccgcaactg
       61 gatactttcg gtcgttaata acaaccaatg cgttattgga aagtgaacga tgaaagccgt
      121 ccccataacc gcagggaatt gtagctatcc agctctcttt atttaacttg taagtgtgtc
      181 cgtaagagag ggcgctcccg gcattgactt ttttcatcat gattattgtg gaaaatagag
      241 acatgctctg tttaaatcgc ttgtcgtgag gcgatgccgg gtcgggcatg tatccgtaac
      301 ttataatacc cggacgtacc atatcaaaaa aagtatcggg ataatatagt acagctccac
      361 tgttggccat gtgtaaaaca ggcttacgtc cgagggtttc gcgaacagct tcggtaattt
      421 ttttataatc ggcaatctgg ttattgttaa aggggttgct actttcgtca gagacgggca
      481 tgtgagtata aaagccttcg atgaaaatat tttcaagctg gtttattatg ttacaagtat
      541 gaacactctc agaggcagga cagccaagtc gccccatgcc ggtatcggtt tttatatgta
      601 tctttatttt tatgttatgc gcgccagcga caagattaca tctttgagca gtgtctatat
      661 cacctatagt gggcatcagg ttgtacttaa ttagcgcgtt tatctgttca tcaccaggga
      721 tgatgtaaga caatattagt atgggtaaat taattccatt ttctcttaag cagatgccct
      781 cttcaattga ggcgactccg aacatattaa taagcccact cttttccgcg aaacgggctg
      841 tctcgatcat tccgtggccg taggcatctg ctttaacggg caatagtatg gggcggtttg
      901 aagtaatatt atgaattgtt tccagattat ttttgatagc ttctgtatca atttcaagcc
      961 atgtgccacg atagttcatt ttgttagtcc cttgatattg ttattaaagc ttgaccctca
     1021 gctagttcga taatctgctt tctgtcaaca gcaaaattac taatgcatag ggtagaaccg
     1081 ggagttaccc gtttgtcgga aagtttataa tgaagacctt cagtattttt tacaacaata
     1141 tcgccaaaga ggggaattat cgagatgtag gctccggcgt gagcctcaag ttcaattata
     1201 tcggtgcaaa agaatgcttc gttattttca tccacaagcc ggatcttgat atcccttcct
     1261 aatttcgcca atagcataat gttcccgatt gtgtggtcaa ggcgggttcc gctaaaacca
     1321 gcgactgtca ggttcttaaa tccattttgt atgcagtgat gcagagccag ttccgagtcg
     1381 gtcatatctt tgtcggcagg gaaagagatt acttcaatat cattttcggt tatatgctcc
     1441 atatgttcgg gttcgataga gtcaaaatca ccaagtgcgg tatctatttt gtatcccaaa
     1501 ttgatggcgt ggccagcacc cccgtctacc gcgataacgg tagggttttc actcattaag
     1561 gaggctagag cctgtttaag tactgtgtag tctgttatgc ggccgttaca taatattaga
     1621 gcctttttca tttaggcggt tccttgaaaa tatttatttt taaaaaaaat ctgttgtaag
     1681 ttattaataa atttttttgg tattgataga aatagactga taatagtatt ggtatttgta
     1741 caaattgagg gtattaaatg ctttgtccat acttttttgt tgcattggat aaagtgttga
     1801 tatagtataa tcagagttaa gctcaataag gtcatgaacc ggttttgtac cgggtaatta
     1861 atttatttag gaagacgtga tgataaaacc ggaacttttg gatcgatgga agattaaaaa
     1921 ctcggaggat ctgtacggaa taaaaacctg gggagcatct tatttcgaga ttaatgataa
     1981 gggtgaagtc tcagtgactc cattcgcgga taagcctgat gtttctgtta gtcttgtaga
     2041 tataatcaag ggaataaaag agcggggact tgatgctcct gttttgctca ggtttgagaa
     2101 cttgcttgat tctcaactga cttttttaaa cgaaagcttt aatgccgcta ta
//
LOCUS       DAOTGZ010000339         2144 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_3064
            NODE_60992_length_2144_cov_4.307803, whole genome shotgun sequence.
ACCESSION   DAOTGZ010000339 DAOTGZ010000000
VERSION     DAOTGZ010000339.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868088
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 2144)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2144)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:22:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,785
            CDSs (total)                      :: 2,751
            Genes (coding)                    :: 2,732
            CDSs (with protein)               :: 2,732
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 12 of 19
            Pseudo Genes (internal stop)      :: 5 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2144
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_60992_length_2144_cov_4.307803"
                     /isolate="AMR_MDS_3064"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..893
                     /locus_tag="P5123_13790"
     CDS             <1..893
                     /locus_tag="P5123_13790"
                     /inference="COORDINATES: protein motif:HMM:NF014075.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF115 domain-containing protein"
                     /protein_id="HRX17379.1"
                     /translation="SLNRGVLNERLFAFSRNLTKNAALISKHGGLAKVLNCFAGVHVL
                     IVGAGESLSKCLPVIKKLHTRDDFVLIAVDMAYGALVNNGITPDFAITCETSPRDFFL
                     PFDTSNTRLLAFSCSCSSNIRNWQGSISFYNWLIREEPYESLWKRAGYDLGFVSTGSV
                     VTTQALSIVMALPALSITLAGNDLGFTDYPYCMGVSWYTNIMRTANRFNPAENAARKT
                     IRLARDYEIKRESRVYFTNNQFLGAKYWIEDLLKVSKKNVYDLSDPGVSGKYINKITP
                     KQLEKMIIGDRENRKKRRTK"
     gene            890..1102
                     /locus_tag="P5123_13795"
     CDS             890..1102
                     /locus_tag="P5123_13795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008118151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar FlbD family protein"
                     /protein_id="HRX17380.1"
                     /translation="MIEVHRLNGTAFYINCDHIETMEETPDTVIHLTNDKTYVVTEKA
                     EEIIDKIIVYKNNIVKPIIKKNSFLE"
     gene            1204..>2144
                     /locus_tag="P5123_13800"
     CDS             1204..>2144
                     /locus_tag="P5123_13800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005034804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylacetate--CoA ligase"
                     /protein_id="HRX17381.1"
                     /translation="MFNREFEAMPLAEMKKLQLERLKWSLNHAYKNNAYYARKFDASG
                     FDPSSFKSLDDMKRVPFLTKQDMRDNYPFGLFAVPLKDVVRVHSSSGTTGNATVVGYT
                     SKDIDVFAEVVARSLSSYGATSDDILQVAYGYGLFTGGLGLHYGGEKLGCLTVPISGG
                     NTERQLMLIKDFGTSVLACTPSYALNIADYLEKKRPDYDIKKTSLRIGVLGAEPWTEA
                     MRNEIETRLGIEAYDIYGLSEVIGPGVSAECSAKSGMHVFEDHFYVEIIDPDTGEVLP
                     EGEKGEIVYTSLTKYACPVIRYRSRDITRLYHDSCSCG"
BASE COUNT          628 a          383 c          473 g          660 t
ORIGIN      
        1 cctctttaaa ccggggtgtt cttaacgagc ggctttttgc ttttagtcgt aatttgacaa
       61 aaaatgccgc tctaatttca aaacatggcg ggcttgccaa ggtgttgaac tgttttgctg
      121 gggttcatgt cctaatagtt ggggctggcg agtcactttc taaatgttta cctgtaatta
      181 aaaaattgca tacccgtgat gattttgtgc ttattgctgt tgacatggcc tacggtgcgc
      241 ttgtaaataa cgggattact ccagattttg cgattacatg cgaaacctca cctcgtgatt
      301 tttttttgcc ctttgacact tcgaatacac ggttacttgc tttttcttgc agttgcagta
      361 gcaatatacg caattggcag ggatccattt cgttttataa ctggcttatt cgagaagagc
      421 cgtatgagtc tctctggaaa cgcgcgggat acgatcttgg ttttgttagc acgggaagtg
      481 ttgtaacaac ccaggccttg tctatcgtta tggccttacc agctttatcg ataactctcg
      541 ccggtaacga tttgggtttt accgattatc catattgtat gggtgtttct tggtatacca
      601 atattatgcg tacagctaat cgctttaatc ctgctgaaaa tgctgcccgt aaaacaatac
      661 gtcttgctcg tgattatgaa attaaacgtg aaagcagggt atactttacc aataatcaat
      721 ttcttggagc aaaatactgg atagaagatt tacttaaagt atcaaaaaaa aatgtgtacg
      781 atttaagcga tcctggtgta tcaggaaagt atataaacaa aataacacct aagcaacttg
      841 aaaaaatgat tatcggtgat cgggaaaacc gtaaaaagag gagaacaaaa tgatcgaagt
      901 gcacagactt aatggtacgg ctttctatat taattgtgat catattgaaa ccatggaaga
      961 gactcccgat accgtaatac atcttaccaa cgacaaaacc tatgtagtga ctgaaaaagc
     1021 tgaggagatc atagacaaaa taattgtgta taaaaacaat atagtaaagc caattataaa
     1081 aaagaactcc tttttggaat agtaacaccc ataaaaattc tattgacact tgaaaacaaa
     1141 ttccgtccaa ttagacggaa tttgttttta ctatccgttt aaaattttgt tttggagaga
     1201 gccatgttta acagggaatt tgaggcaatg cctttggctg agatgaaaaa acttcagctt
     1261 gagaggctta aatggtcact gaatcatgct tataaaaaca acgcttatta cgcgcgtaaa
     1321 tttgacgcgt ccggttttga tccatcttcg ttcaagagtc ttgacgatat gaagagggtt
     1381 ccatttttaa caaaacagga tatgcgcgac aactatccct ttggtttgtt tgctgtacca
     1441 cttaaggatg ttgtgcgtgt tcactcttct tctggaacaa ctggaaacgc tactgttgtg
     1501 ggttatacat caaaggatat tgatgttttc gcggaggttg ttgcccggtc actgagctcg
     1561 tatggtgcta ccagtgatga tatactgcaa gttgcttatg gttatggtct ttttacgggt
     1621 gggctggggc tccattatgg tggagaaaaa cttggttgtt taactgtacc tatctctggc
     1681 ggaaacaccg agcgtcaatt aatgcttata aaggattttg gaacatcagt tttagcctgt
     1741 acaccgtctt atgccctaaa tatagcggac tatcttgaaa aaaaacgtcc tgattatgat
     1801 atcaaaaaga cttcgcttag aataggggtt ctgggtgcgg aaccgtggac cgaggcaatg
     1861 cgtaacgaga ttgaaactcg tcttggtatt gaggcttatg atatatacgg tttaagtgaa
     1921 gtaataggac ctggagtatc ggcagaatgt tctgctaaaa gcgggatgca tgtgtttgaa
     1981 gaccattttt atgtagagat tattgaccca gatacaggag aggttttacc agaaggtgaa
     2041 aaaggtgaga ttgtgtatac ttcacttacc aaatatgcct gtcccgtaat cagataccgg
     2101 tcacgagata ttacgcgact ttatcatgat agctgctcat gcgg
//
LOCUS       DAOTGZ010000340         2054 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_3064
            NODE_64683_length_2054_cov_3.812406, whole genome shotgun sequence.
ACCESSION   DAOTGZ010000340 DAOTGZ010000000
VERSION     DAOTGZ010000340.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868088
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 2054)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2054)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:22:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,785
            CDSs (total)                      :: 2,751
            Genes (coding)                    :: 2,732
            CDSs (with protein)               :: 2,732
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 12 of 19
            Pseudo Genes (internal stop)      :: 5 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2054
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_64683_length_2054_cov_3.812406"
                     /isolate="AMR_MDS_3064"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            1..123
                     /locus_tag="P5123_13805"
                     /pseudo
     CDS             1..123
                     /locus_tag="P5123_13805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007637203.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate racemase"
     gene            420..1337
                     /locus_tag="P5123_13810"
     CDS             420..1337
                     /locus_tag="P5123_13810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004752038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1837 domain-containing protein"
                     /protein_id="HRX17382.1"
                     /translation="MEFNILFDCAFSEINLDKTLAPIDKKFSVSYLNDFEDGQWRYSK
                     FQSFIWDNIAETSLSHKERESLVNHSLLVAAAKNLRLTDSDKEDIGRGSELAEIVLYG
                     LMKHHYQALPVVPKIFYKQNSQDNAKGADSVHIVLEPNDSYSIWFGEAKFYNSIEDAR
                     LSSIVKSVENSLKTDKLKKENSIITNVQDLDDLVLDESLKEKIKQTLSNKTSIDELKP
                     RLHIPILILHECEITKKATKLDDNYLEEVRLFYRDRATSYFKKQIIQLKTSVHLYSDI
                     TFHIILFPVPSKENIVDKFVVNVQHYKDQ"
     gene            1339..>2054
                     /locus_tag="P5123_13815"
     CDS             1339..>2054
                     /locus_tag="P5123_13815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012667766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HRX17383.1"
                     /translation="MDIFEACSNINDHIQSGNDLSARNELIKVLDFHEKEGISYSPLV
                     NHLIREAGLYPYLDPETSSWQDRFIYESFKVNIGLELPVTLHREQSLLLKKLLEGKSL
                     AVSAPTSFGKSFVIDAFISINRPKNIAIIVPTIALADETRRRLQQKFSDEYKIITTVN
                     IELADKNIFIFPQERAILYIPKLESIDILVIDEFYKASADYDKERAPSLLRAMIQLGK
                     ISKQKYYLAPNIATLNDTPFT"
BASE COUNT          688 a          403 c          403 g          560 t
ORIGIN      
        1 agttgggaaa gcatggttgg ctactatcgt gcgataaacg aaggtgtaaa gaataaactc
       61 ggaggtttgt attcagccaa gattgtcatg tatagcgtcg atttcgatcc catagaaaag
      121 ctgctgtgag atatgcgttt ttgatgtttt catagcaaaa ctcaacgacc accagctaaa
      181 gctggtgggt tcttgcgccg ggcgcagggg actgaaagtc cacccatcac agcgcccggc
      241 gctttaagaa cggcatctct aaagcgatat gattggctct aagtcatgtt cttttgaagg
      301 gcatctgagt ccgcctggag agttgagctg aaagctgacc ggctaaagcc ggtggtttta
      361 accttacata ttgaaataaa aaaacttaac tgagccaacg tagaaaatag cggtaagaca
      421 tggaatttaa tattttattt gattgcgctt tttcagagat taaccttgat aagactctcg
      481 ctccgataga taaaaaattc agcgtaagct acctgaatga tttcgaagac ggccagtggc
      541 gatattcgaa gtttcaaagt tttatttggg ataacattgc tgaaacttct ctctcccaca
      601 aagaacgtga gtcattagtt aatcactcct tgctcgtagc tgcggcaaaa aacttacgcc
      661 ttacggactc tgataaagaa gatattggcc gaggtagcga actggctgaa atcgtactat
      721 acgggttaat gaagcaccac tatcaggcgt tgcccgttgt ccctaagata ttctacaaac
      781 aaaactcaca agataatgcc aaaggtgcag atagtgtgca tatagtacta gaacctaatg
      841 attcatattc tatttggttc ggcgaggcaa aattctataa cagcattgaa gatgcacgac
      901 tatctagcat tgttaaatca gtagaaaact cactaaaaac agacaagctg aaaaaagaaa
      961 attccataat aactaatgtt caggaccttg atgaccttgt tcttgatgag agcttgaagg
     1021 agaaaataaa acaaacactt tctaataaga cctctatcga cgagttgaaa ccacggcttc
     1081 acataccaat tttgatcctt catgagtgtg agataaccaa aaaagcgacg aagttggatg
     1141 ataactattt agaggaagtt cgattatttt atcgcgacag agcgacgagt tacttcaaaa
     1201 aacaaatcat acagctcaaa acttctgttc acttatactc tgacataact tttcatataa
     1261 ttctctttcc tgttccaagc aaagaaaata tcgtagacaa gttcgtggta aacgttcaac
     1321 actacaagga tcaatgacat ggatattttt gaagcctgta gcaacatcaa tgatcatatc
     1381 cagagcggga acgatttgtc agcacgaaat gagctgataa aggttttaga ctttcatgaa
     1441 aaagaaggca tttcttactc gcctctcgtg aatcatctaa tccgcgaagc tgggctgtac
     1501 ccctatctcg atcctgaaac atctagctgg caagatagat ttatatacga atctttcaaa
     1561 gttaatattg gccttgaatt acctgtcaca ttacatagag aacaatctct attactaaaa
     1621 aaattactgg aaggtaaaag cctagctgtt agcgccccaa ccagcttcgg aaaaagtttt
     1681 gtgattgatg cattcatatc aataaataga ccaaagaata tcgcgattat tgtcccgaca
     1741 atagcccttg cagatgaaac aagaagaaga cttcagcaga aattttcgga tgaatacaag
     1801 ataattacaa cagttaatat agaattggca gataaaaata tatttatctt tcctcaggaa
     1861 agagcgattt tatacatccc aaagctagaa tctattgata ttcttgttat tgatgaattt
     1921 tacaaggcaa gtgcagatta cgataaagaa agggcgcctt cgttactgcg agcgatgatt
     1981 cagcttggta aaatctcaaa acagaaatac taccttgccc ccaatatagc cacgcttaat
     2041 gacaccccat tcac
//
LOCUS       DAOTGZ010000341         2041 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_3064
            NODE_65227_length_2041_cov_4.627895, whole genome shotgun sequence.
ACCESSION   DAOTGZ010000341 DAOTGZ010000000
VERSION     DAOTGZ010000341.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868088
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 2041)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2041)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:22:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,785
            CDSs (total)                      :: 2,751
            Genes (coding)                    :: 2,732
            CDSs (with protein)               :: 2,732
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 12 of 19
            Pseudo Genes (internal stop)      :: 5 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2041
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_65227_length_2041_cov_4.627895"
                     /isolate="AMR_MDS_3064"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..931
                     /gene="tgt"
                     /locus_tag="P5123_13820"
     CDS             <1..931
                     /gene="tgt"
                     /locus_tag="P5123_13820"
                     /EC_number="2.4.2.29"
                     /inference="COORDINATES: protein motif:HMM:TIGR00430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008479 - queuine tRNA-ribosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="tRNA guanosine(34) transglycosylase Tgt"
                     /protein_id="HRX17384.1"
                     /translation="YIRPGVDVLDAAGGLHKFMNCKLPILTDSGGFQVFSLSDLCKVR
                     EDGAEFRSHLDGSKHLFTPRSVLDVQRSIGSDIMMVLDQCVEYPADHSYAREACERTI
                     RWAGEAIDYYRESFDKERQKIFAIVQGSTYPDLRKDCAKRLADLDFPGYAIGGLSVGE
                     PKDLYNEMSEITTAELPEDKPRYMMGVGSPLEIIESVSHGIDMFDCVMPTRIARNGTL
                     YTSQGRINIRNSQYEKDFTPLDPECDCYVCRNFSKSYLRHIFRVGEISALIYNTYHNL
                     SYMKKLMMRVRSSIENGNFEELRREIHSYYGRP"
     gene            932..>2041
                     /locus_tag="P5123_13825"
     CDS             932..>2041
                     /locus_tag="P5123_13825"
                     /inference="COORDINATES: protein motif:HMM:TIGR00200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="competence/damage-inducible protein A"
                     /protein_id="HRX17385.1"
                     /translation="MKCSIVTIGTEILLGDIINTNSAYLSRKLAEHGIAVFSHHTVGD
                     NPDRIQDTFKTAFKNCDTVITTGGLGPTEDDITREAVARFLGLELVSDAKAQTEIATF
                     FEQRGRSMSPSNLKQAMFPEGSIICHNSRGTAPGAIASSNGKTVIMLPGPPFELKLMF
                     QNSVLPYIEKLSSQVFRSRVIHFADIGESEMNDRVKDLLDGKNPTVAPYAKTSGCQLR
                     ITAGAKDHDHAMKLIKPLEDEIVNRLPEYIYGYDDDTLESRATELLKKSSMTISCAES
                     CTGGMLSSTLVNYAGISDVFVGSVISYSNTVKIKELSVEEGILEKNGAVSEECARAMV
                     IGCAQKFNTDVAISITGIAGPGGATDDKPVGTVYTG"
BASE COUNT          635 a          345 c          464 g          597 t
ORIGIN      
        1 ttatatacgt ccaggagttg atgtgctcga tgctgctgga gggttgcata agtttatgaa
       61 ttgcaaactg ccgattctaa cggattcggg tggtttccag gttttctctc tgtcggatct
      121 ttgcaaagtg agagaagacg gtgcagaatt ccgttctcat ctggatggat cgaaacacct
      181 ttttacacca cgttcggttc ttgatgtaca aagatcaatc ggctcggaca ttatgatggt
      241 acttgatcaa tgtgtagaat atccagctga ccactcgtat gcgcgagaag catgcgaaag
      301 aacaataaga tgggcaggcg aagctatcga ttattacaga gaaagttttg ataaggaacg
      361 ccaaaagata ttcgccatag tacagggatc gacttatccc gatttacgta aagattgtgc
      421 aaaacgactt gctgatcttg atttccctgg atatgctata ggcggattgt ctgtaggaga
      481 acccaaagat ctttataacg aaatgtcaga gataacaaca gctgaactac ctgaagataa
      541 accacgatat atgatgggtg taggttcgcc acttgaaata attgaatcgg tttcgcatgg
      601 aatagacatg tttgattgtg taatgccaac tagaattgcg cgtaatggga cactttatac
      661 ttcgcaagga cgtattaata tcagaaatag ccaatatgaa aaagatttca caccacttga
      721 tccagaatgt gactgttatg tatgtcgcaa tttttcaaaa tcttatctca ggcatatttt
      781 tagagtgggt gaaatatcgg cacttattta taatacatat cacaatctta gttatatgaa
      841 aaaactaatg atgagagtaa ggagttctat agaaaatgga aattttgaag aattaagacg
      901 tgaaatacac agctattacg ggaggccgta aatgaagtgc tcaattgtaa caatcggtac
      961 ggagatttta cttggtgata taattaatac aaattcagcg tatctttctc gaaagcttgc
     1021 ggaacatggt atagctgtat tttcgcatca tactgttggg gataatcctg atcgtattca
     1081 agatactttt aagaccgctt ttaaaaactg tgacacagta attacgacag gtggacttgg
     1141 cccaacagag gatgatatta cgcgtgaagc ggtagctcgt tttctggggc ttgaacttgt
     1201 ttctgacgca aaggctcaaa ccgaaatagc aacttttttt gaacagaggg gacggagcat
     1261 gtcaccttcc aatcttaaac aggcaatgtt tccagagggc tctattatct gtcataatag
     1321 taggggaact gcacctggag cgattgcttc atctaatggc aaaaccgtaa taatgctgcc
     1381 gggaccaccg tttgagttga aactgatgtt tcagaattca gttttaccat atatagaaaa
     1441 acttagttcg caggttttca gatccagagt aattcatttt gcagatatag gtgaatctga
     1501 aatgaatgat agagtaaaag atcttcttga tggcaaaaat cctacagttg ctccatatgc
     1561 gaaaacatca ggatgtcagt taagaataac tgctggtgct aaagatcatg atcatgcaat
     1621 gaaattgata aaaccgctcg aagacgaaat tgtcaacagg cttcctgaat atatttatgg
     1681 atatgatgat gataccttgg aaagtcgtgc tacagaatta ttaaaaaaat catcaatgac
     1741 aatatcctgt gctgaatctt gtaccggtgg tatgctctcc tcgactcttg ttaactatgc
     1801 gggaatttcg gatgtttttg tgggctctgt tatttcttat agcaatacgg taaaaataaa
     1861 agaactttcg gtagaagagg gtattctgga aaaaaatggg gcggtttccg aagagtgtgc
     1921 aagagcaatg gttattggtt gtgcacaaaa atttaataca gatgttgcga tttctataac
     1981 gggtattgct ggtcccggag gggcaactga tgacaaaccg gttggcactg tgtatactgg
     2041 g
//
LOCUS       DAOTGZ010000342         1994 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_3064
            NODE_67315_length_1994_cov_4.904074, whole genome shotgun sequence.
ACCESSION   DAOTGZ010000342 DAOTGZ010000000
VERSION     DAOTGZ010000342.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868088
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 1994)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1994)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:22:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,785
            CDSs (total)                      :: 2,751
            Genes (coding)                    :: 2,732
            CDSs (with protein)               :: 2,732
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 12 of 19
            Pseudo Genes (internal stop)      :: 5 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1994
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_67315_length_1994_cov_4.904074"
                     /isolate="AMR_MDS_3064"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            69..500
                     /gene="umuD"
                     /locus_tag="P5123_13830"
     CDS             69..500
                     /gene="umuD"
                     /locus_tag="P5123_13830"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006921240.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translesion error-prone DNA polymerase V
                     autoproteolytic subunit"
                     /protein_id="HRX17386.1"
                     /translation="MTRLTLHRNEETPTVIERPLFVSSVQAGFPSPAEDYIESSLDLN
                     AHLIKNPAATFFVRVSGSSMKDALIFPGDILLVDRSITPRSGRVVIAVIDGELTVKRL
                     LLNRTRPVLKAENCEFADIDIAPESEFEIWGVVTHIIHEAL"
     gene            500..1774
                     /locus_tag="P5123_13835"
     CDS             500..1774
                     /locus_tag="P5123_13835"
                     /inference="COORDINATES: protein motif:HMM:NF013015.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Y-family DNA polymerase"
                     /protein_id="HRX17387.1"
                     /translation="MFLLADCNNFYVSCERVFNPSLRDRPVIILSNNDGCAVALSNEA
                     KALGVPRGAPLFKWRDFLLKNNGVWLSSNYTLYGDMSRRVMDILSSFSEDCEVYSIDE
                     AFLRIDISDEKTIQKIARELIETVYKYTGIPLSVGSANTKTLAKAANHYAKKTAKTGF
                     ACITPANRESMLQQIPVSQIWGIGYRHDKLLKSHGVNNALQFTKLCETWIKKRMSITG
                     LRTHKELNGTPCIDLQHVPPSRQSIVSSRSFGTPVSSFNLLNESIHEYGYRAAARMRS
                     MKLVCSSLSVYLCTNRFKDEPQYSNSASVSLEYPTNSSRTITYQAEVILKAIYRSGYK
                     YKKTGIMLHGLIENSEQQMSLFEKKDYMNRDEKLMSVMDSINRRYGSKSVILASEGIS
                     RNWQMKREFLTPSYTTRWSDIPLVKVESNKMF"
BASE COUNT          621 a          432 c          384 g          557 t
ORIGIN      
        1 tcttgcctgt ttatacaata tttataaatt ggtaataact acacatatgt ttagcacaag
       61 gagaatatat gacccgttta accttacatc gtaacgaaga aaccccaacc gtaattgaac
      121 gccccctttt tgtcagctcc gttcaggctg ggtttccgtc tccggccgaa gactatatcg
      181 aaagctctct ggatcttaat gctcacctga taaaaaaccc ggctgccacc ttttttgtac
      241 gcgtgtccgg cagctccatg aaagacgcgc tgatatttcc gggagacatt cttctggttg
      301 accgctcaat cacaccccgc tcgggcagag tggtcatcgc ggttattgac ggagaactga
      361 ctgtcaaacg tttactgctt aatcggacaa ggcctgttct aaaagccgaa aattgcgagt
      421 ttgcagatat cgatatcgct cctgaaagtg aattcgagat atggggagtt gtaactcata
      481 ttattcacga ggcgctgtga tgtttctatt ggccgattgc aataattttt atgtatcatg
      541 cgaacgggta tttaaccctt cgcttcgtga ccgtccggta attatacttt caaataatga
      601 cgggtgcgct gtcgcgctct caaatgaagc aaaagcgctg ggagttcccc gtggagctcc
      661 actttttaaa tggcgcgatt ttcttttaaa aaataacgga gtctggctct cttcgaatta
      721 tactctttat ggtgacatgt ctcgccgtgt tatggatatt ctgtcatctt tttcggaaga
      781 ttgcgaagta tattcaattg acgaagcctt tttaagaatt gatatttcgg atgagaaaac
      841 catacaaaag atagcccgcg aattaataga aactgtttac aaatacaccg gaattccact
      901 ttcggtaggc tctgctaata ccaaaaccct ggccaaggct gcaaatcact acgcaaaaaa
      961 aactgcgaaa acaggttttg catgtatcac accagctaat cgggaatcga tgctacaaca
     1021 gattccggtc tcacaaatat ggggcatagg ttatcgtcac gataaacttt taaaaagcca
     1081 cggcgttaat aacgcgttac agtttacaaa attatgtgag acatggatca aaaaacgcat
     1141 gagtataacc ggtcttcgca cccataaaga gcttaacggc accccttgta tcgacttgca
     1201 acatgtgcct ccgtcccgtc aatccattgt ttcatcgcgt tcgtttggaa cccccgtaag
     1261 ttcctttaac ctgcttaacg aatcaataca tgaatacggc tatcgtgctg cagcccgtat
     1321 gcgatcaatg aaacttgtct gctcttcact ttcagtttat ctttgcacaa acaggtttaa
     1381 agacgaacca caatattcaa actcggcatc ggtatctctg gaatacccta ccaattcaag
     1441 tagaacaata acttaccagg cagaagtcat acttaaagca atttatagat ctggatataa
     1501 atacaagaaa acaggaatta tgcttcacgg cctgattgaa aacagcgagc aacagatgag
     1561 tctttttgaa aaaaaagatt atatgaatcg tgacgaaaaa cttatgagtg taatggattc
     1621 tataaaccgt cgttacggat caaaaagcgt cattcttgct tcggagggca ttagccgcaa
     1681 ttggcagatg aaacgtgaat ttttgacccc tagttacaca acccgttgga gtgatattcc
     1741 gttggtgaaa gttgaatcca ataaaatgtt ctgattctat tactacgttt atacgactgt
     1801 aaccttattc aaaaccaata ctataaagac ggttattcgc tttagatttt cgggctattc
     1861 gcccgaaatc gtaagaaaaa aagacaacta ataacgaata atgaaaaatt aataatgatt
     1921 attgcaaaaa caaaaatagc gcaaagtcta aaaccgttta aaacaaaatc cataacccca
     1981 gcacttcaag ataa
//
LOCUS       DAOTGZ010000343         1991 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_3064
            NODE_67444_length_1991_cov_5.363120, whole genome shotgun sequence.
ACCESSION   DAOTGZ010000343 DAOTGZ010000000
VERSION     DAOTGZ010000343.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868088
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 1991)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1991)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:22:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,785
            CDSs (total)                      :: 2,751
            Genes (coding)                    :: 2,732
            CDSs (with protein)               :: 2,732
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 12 of 19
            Pseudo Genes (internal stop)      :: 5 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1991
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_67444_length_1991_cov_5.363120"
                     /isolate="AMR_MDS_3064"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..273)
                     /locus_tag="P5123_13840"
     CDS             complement(<1..273)
                     /locus_tag="P5123_13840"
                     /inference="COORDINATES: protein motif:HMM:NF024571.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HRX17388.1"
                     /translation="MRKIRPFINTPVIKVFTGIRRCGKSVMLELVQAELKKQAINDKQ
                     IISLNFESASDGRVRTLDSILATIHEQSSKLNNRRLYLFFDEIQELI"
     gene            985..1119
                     /locus_tag="P5123_13845"
     CDS             985..1119
                     /locus_tag="P5123_13845"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRX17389.1"
                     /translation="MNDKINKNIDDKKSKLEDCVKPNNAEAQRNSDQDEPCHEAVDGK
                     "
     gene            complement(1136..1657)
                     /locus_tag="P5123_13850"
     CDS             complement(1136..1657)
                     /locus_tag="P5123_13850"
                     /inference="COORDINATES: protein motif:HMM:NF025052.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HRX17390.1"
                     /translation="MKILERLKSLDLLLICGSYGAGKNELANKYFNNGQWNRVSRNEI
                     RKLMFEMTRFGSQWTAESFNEEDDVLVKHIERKILEHYLHNKRKVLVINTFATIKSRQ
                     RFLSIAKNFGRKTGAIYLNMPLDYCLSRYKTGNPTLPETVIRSIYSRTELPDKKEGFE
                     EVLIINPKEQNRQ"
BASE COUNT          630 a          339 c          378 g          644 t
ORIGIN      
        1 aattaactcc tgtatctcgt caaaaaaaag gtacaaacgt ctgttattaa gtttagaaga
       61 ttgctcgtga atggttgcaa gtatagaatc tagtgttctt acgcgtccgt ctgatgcgga
      121 ttcaaagttg agagagatga tttgtttgtc gtttatggcc tgctttttta actcggcctg
      181 aacaagttca agcatcaccg acttgccaca acgtcgaata cctgtaaaaa ctttgattac
      241 cggagtattt ataaatgggc ggattttccg catatatagt tcgcgtgata tcatttagta
      301 aataaaatac agttatatcg aaaaatcaag taaaaaacac aaaaacattt cgatatatcg
      361 aaaaaacaat agataaaaac gtaattcttt cgatataacg aaagccaagt tgcaatctat
      421 gtagtaatta aagatgaaac cgtgaaacat tactttgaag cagctggtaa gatttatgca
      481 gaacgcgctc ttatttcgcc tttatcccct taaaaaactg atctgcttct tcagatagtt
      541 tttttagtgc taaaatggta cattctactt ctgcttttcg ttttgcattc attaaatgct
      601 tcatcatcac tacttttata gatcgttgca tgcttctctg atattgttgt cgagaatgga
      661 atcaggccag ttcttgaatc cagcaaaaag gcttctacag ttgaaaatgc cttaatgtca
      721 ttgggtttaa aaaacggtat ttctcaaaga tatcactttg aacccggtag actaaaatta
      781 gattgccctg catgtgtaat gtggcttctc ttaaattgga aatagtaatt ttatcgggct
      841 tgataaacga aggaataaag tagctttaca tgctgcatat aaagtcgatt gcgtaggata
      901 aaaacaaaat agtgtgcttt taagtcgcat tgacaaactg taaaaatttc agtatacttc
      961 aggtaggtta ttttggagga tattatgaac gataaaatta ataaaaatat tgatgataaa
     1021 aagtcaaagc ttgaagattg tgttaagcct aacaatgcag aggcgcaacg taatagtgat
     1081 caagatgagc catgtcatga agctgttgac ggcaaataga gagtctatga ttcgctcatt
     1141 gccggttttg ctctttggga ttaataatca gaacctcttc aaagccctct tttttatcgg
     1201 gcagctctgt tcttgagtat atcgatctta ttaccgtttc gggcagggtg gggttccctg
     1261 ttttgtatcg tgaaagacag taatccaggg gcatattgag atatattgct cctgttttgc
     1321 ggccaaagtt ttttgcgatg gatagaaatc tttgacgtga ctttattgtc gcaaaggtat
     1381 taattaccag aacctttctt ttattatgca gatagtgttc cagaattttg cgttcaatat
     1441 gcttaaccag tacatcatcc tcttcattaa agctttcggc agtccactgg cttccgaaac
     1501 gcgtcatctc gaacatgagt ttgcgtattt cgtttcggct aacacggttc cactggccgt
     1561 tgttgaaata tttatttgcc aattcgtttt ttcctgcacc gtaagacccg caaatcagca
     1621 aaagatcaag tgatttaaga cgttccagta ttttcattat attctccaaa acatcagact
     1681 ttagtcgcat ggttgttcgg gaagtattct gtatgaaaaa aatcatttgt taccctaaag
     1741 catataacac aactgtgctt aaaatgcaaa taataattgc agtgattaag aaattaacaa
     1801 caacggtcaa atagcatgtt ataaaattct gaagcacgaa atgttattct gccaggagct
     1861 gataattacc ggaataattt tgaaagttta gcgcatatga agattcatct tgagcagcta
     1921 aaaaacagat tagcagaggt gttatagata gcgtatcgaa gaagaaagct tttaaataat
     1981 catcatttct g
//
LOCUS       DAOTGZ010000344         1989 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_3064
            NODE_67544_length_1989_cov_3.623578, whole genome shotgun sequence.
ACCESSION   DAOTGZ010000344 DAOTGZ010000000
VERSION     DAOTGZ010000344.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868088
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 1989)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1989)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:22:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,785
            CDSs (total)                      :: 2,751
            Genes (coding)                    :: 2,732
            CDSs (with protein)               :: 2,732
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 12 of 19
            Pseudo Genes (internal stop)      :: 5 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1989
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_67544_length_1989_cov_3.623578"
                     /isolate="AMR_MDS_3064"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1127)
                     /gene="purB"
                     /locus_tag="P5123_13855"
     CDS             complement(<1..1127)
                     /gene="purB"
                     /locus_tag="P5123_13855"
                     /EC_number="4.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014857355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004018 - N6-(1,2-dicarboxyethyl)AMP
                     AMP-lyase (fumarate-forming) activity [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate lyase"
                     /protein_id="HRX17391.1"
                     /translation="MIERYSRKEMSNIWNLENKFRIWLDIEIAACEANNKLGIVPDKD
                     LAIIKDKADFDLNRIHEIEAETHHDVISFLTAVAEKVGEPSRFIHYGLTSSDIGDTAL
                     CIQMRQAGELLVKELDRLIEALKEQAVKHKFTPCMGRSHGVHAEPTTFGMKMLLFYEE
                     CKRNRVRLERAIENISFLKMSGAVGTFSNISPEVESMMAEKLNLTPDPVSTQVIQRDR
                     HAEYAAALAITAATLDKMATEIRHLQRTEVREAEEPFQKGQKGSSAMPHKRNPIICER
                     VSGISRVIKSNMFTALDNITLWHERDISHSSAERITLPDSTIALDYLLDKMTFVISGL
                     HIYPEAMLDNINKTGGLFFSQSLLLSIIDKNVSREDAYKMVP"
     gene            1249..>1989
                     /locus_tag="P5123_13860"
     CDS             1249..>1989
                     /locus_tag="P5123_13860"
                     /inference="COORDINATES: protein motif:HMM:NF015983.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HRX17392.1"
                     /translation="MQLKIGYDNRIIRQKNCAGKVKRRQGDMYKYIFGPVVSRRLGLS
                     LGIDLVPFKTCPFDCVYCECGATTNLTVKRAEYVPVNDVCAEIDMYMKNAKSHPDYIT
                     FSGSGEPTLHTGIGDIIKFIKSTFPNQKIALLTNAALLDDSSLRHDILGCDIIVPSLD
                     AVSQQVFKNILGPHLSLDPQKIVNSIAELKKEYSGKLVVELFIVPGQNDTPEELTLIK
                     SALDKIKPDALQINSLDRPGTSSPVTKPD"
BASE COUNT          552 a          380 c          423 g          634 t
ORIGIN      
        1 ggaaccattt tgtatgcgtc ttcgcgtgaa acattcttgt cgataatcga aagcagaaga
       61 ctctgggaaa aaaagaggcc tcctgtttta ttgatattgt caagcatagc ttctggatat
      121 atgtgaagtc cggaaattac aaaggtcatc ttatctaaaa gatagtcaag tgctatggtc
      181 gagtcgggta gggttattct ttcggcagag gagtgtgata tgtcgcgctc gtgccatagt
      241 gttatgttat cgagagcggt aaacatatta gattttatta cgcgcgaaat gccagatacc
      301 cgttcacaaa taattgggtt tcttttatga ggcattgcgg atgatccttt ttgccctttt
      361 tgaaacggtt cttcggcttc gcggacttca gtacgctgaa ggtggcgtat ttcggttgcc
      421 atcttgtcaa gggttgcggc agttattgca agtgcggcag cgtattcggc gtgacggtca
      481 cgctgtatta cctgtgtcga tacaggatca ggtgtcagat taagtttttc agccatcatt
      541 gattcgactt caggtgatat attgctgaat gttccaaccg cacccgacat tttgagaaaa
      601 ctgatgtttt cgattgccct ttcaagccgt acacggtttc gtttgcactc ttcataaaaa
      661 agaagcattt tcattccaaa ggttgtgggt tcagcgtgaa caccgtgaga acgtcccata
      721 catggagtaa acttgtgttt aacagcctgt tctttaagtg cttcgataag gcggtcaagt
      781 tctttaacca gaagttctcc tgcctgacgc atctgaatgc aaagagctgt gtctccgata
      841 tcgcttgagg ttaaaccata gtgtataaaa cgacttggtt cgccgacttt ttcagccaca
      901 gcagttaaga atgaaataac atcatgatga gtttcagctt ctatttcgtg aatacgattt
      961 aggtcaaagt cggccttgtc ttttattatt gccagatctt tgtcaggtac aatgcccaat
     1021 ttattattcg cttcacaagc ggcaatttct atatcaagcc agattctgaa tttgttttcc
     1081 agattccaga tattgctcat ctctttacgg ctgtatcttt ctatcatgtt tttctccggt
     1141 tacaggtaat ctgtttttta ttttttgtga atgcttaagt ttgacaaata aatatgcagg
     1201 ttgttaatgg ataaaacctg ataacaaact ctttattggg tacaaaaaat gcaattaaaa
     1261 attggttatg acaaccggat tatccgccag aagaattgtg ctggtaaggt taaaagaagg
     1321 caaggtgata tgtacaaata tatatttggt cctgtagttt caagaaggct tgggttgtcg
     1381 cttggaatag atctggttcc ttttaagaca tgtccttttg actgtgtcta ctgtgagtgc
     1441 ggggcaacaa caaatctgac cgtaaaacga gccgaatatg tgcctgtaaa tgatgtatgt
     1501 gctgaaattg atatgtacat gaaaaatgct aaatcacatc cggattatat tacattttcc
     1561 gggtcaggag aaccaacact gcatactgga attggtgata taattaagtt cataaaatca
     1621 acttttccga accagaaaat tgctctattg acaaacgctg ccctgcttga tgactcatct
     1681 ttgcgacatg atatacttgg ttgcgatatt atagtgccgt cacttgatgc ggttagccag
     1741 caggttttta aaaacatact aggtcctcat ttgtctcttg atccacaaaa aatagtaaac
     1801 tctatagcag agcttaagaa agaatattct ggtaaacttg tcgttgaact gtttattgta
     1861 cctggtcaaa acgatacacc cgaagaactt acacttatta agtcggcttt agataagatc
     1921 aaacctgacg cacttcagat aaactcgctt gatcgtcccg gtacttcatc tccggtaaca
     1981 aaacccgac
//
LOCUS       DAOTGZ010000345         1845 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_3064
            NODE_74876_length_1845_cov_6.182123, whole genome shotgun sequence.
ACCESSION   DAOTGZ010000345 DAOTGZ010000000
VERSION     DAOTGZ010000345.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868088
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 1845)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1845)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:22:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,785
            CDSs (total)                      :: 2,751
            Genes (coding)                    :: 2,732
            CDSs (with protein)               :: 2,732
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 12 of 19
            Pseudo Genes (internal stop)      :: 5 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1845
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_74876_length_1845_cov_6.182123"
                     /isolate="AMR_MDS_3064"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(164..1279)
                     /locus_tag="P5123_13865"
     CDS             complement(164..1279)
                     /locus_tag="P5123_13865"
                     /inference="COORDINATES: protein motif:HMM:NF018455.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HRX17393.1"
                     /translation="MSNEADAKMNGITLEDILADVANLSRSSNQEELFVIRTAKQCLE
                     DAKHMPIPVDLFKGLVYEGELTILFADTGIGKSILAVQICDEISRSHKVAYIDLELSD
                     KQFQKRYSNNYSDEYQFNENLLRVTFNPLGSIPEGKTYDDFFIESLLKIIHDENIKIV
                     VIDNMTRLVSGDTDSAHIAKPLMDRLSDIKHREGLTLLLLEHTRKTDNTRPISLNDLQ
                     GSKMKANFADSIFSIGRSAIGQDIRYIKQLKNRSDEIIYHSEKVLTARITQQGSFLQF
                     VFMEEQPESAHLRHRDNNDYDELVNSIIELSQTGISQREIARELGVSVATVNRRLKNK
                     ELSANNVTPVSPVTGCNGCNSVTVATENQNEQKTIFE"
     gene            complement(1276..1611)
                     /locus_tag="P5123_13870"
     CDS             complement(1276..1611)
                     /locus_tag="P5123_13870"
                     /inference="COORDINATES: protein motif:HMM:NF024139.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HRX17394.1"
                     /translation="MKTSNNFFDRPLYSLTVSEFTELLRSMQRPEPSRKVFGKYLTIS
                     DLMEITGYKKQTIYQKTANKEIPGKRKIDGRVLFETAAILEWIESNAIATTSETFNQL
                     MHSQGRAEL"
BASE COUNT          504 a          390 c          354 g          597 t
ORIGIN      
        1 gaataatgat aaccacattt ttcttctcga tcacaacgcc caacggaaga atgaagctcc
       61 ttgctggtgt cagagtcaat gtatcttaca aaagtctttt gtccgcacct tgggcaaagc
      121 catttgcctc tctttgccag actatattta tattctgcca taactactcg aaaatggttt
      181 tctgttcgtt ctgattttcc gtagctactg ttacactgtt acacccgtta caccctgtaa
      241 caggtgaaac aggtgtaaca ttattagcac ttagctcttt gtttttaagc cgtctattta
      301 ctgtggctac agacactccg agttctctgg caatttctcg ttgagatatg cctgtttgcg
      361 atagctctat aatgctattt accagttcgt cataatcatt gttatcacga tgcctcaagt
      421 gtgcagattc gggttgttct tccatgaaaa caaactgtaa aaaacttcct tgttgcgtaa
      481 tacgagctgt taatactttc tcgctgtgat atataatttc atcactgcga ttttttaatt
      541 gtttaatata cctgatgtct tggccaatag cgcttctgcc tattgagaat atgctatccg
      601 caaaattggc cttcatcttc gagccctgga ggtcatttaa agatattggc cttgtgttat
      661 ctgttttacg ggtgtgttcg agaagaagta aggttaatcc ctctctatgc ttgatgtctg
      721 aaagacgatc cataagaggt ttcgcaatgt gagcagaatc ggtatcgccg ctgactagtc
      781 gtgtcatatt atcaatgacc actatcttga tattctcgtc atggatgatc tttaataaac
      841 tctcaataaa gaaatcatca taggtcttac cttccgggat ggagcctaac ggattaaaag
      901 ttaccctcaa taagttttca ttgaactgat attcatcaga ataattattg gaataacgtt
      961 tttgaaactg cttatccgat agctcgaggt cgatataagc cactttgtga gatctactga
     1021 tttcgtcaca aatctgcacc gccagaatac tttttcctat gccggtatcg gcaaaaagaa
     1081 tagtcaattc cccttcatat actaatcctt taaaaagatc caccgggatg ggcatatgtt
     1141 tagcatcttc aaggcactgc tttgctgttc taatcacgaa taattcttcc tgattcgaac
     1201 ttcttgaaag attggcgacg tctgctagaa tatcttccag agtaatgcca ttcatcttgg
     1261 cgtctgcttc gttactcata actcagccct cccttgactg tgcatcaact ggttaaaggt
     1321 ttctgatgta gtagcgattg cattggattc aatccattcc aaaatagccg ctgtttcaaa
     1381 aagaacccgt ccatcaattt ttcgcttacc cgggatttcc ttgttggcgg ttttttggta
     1441 aatggtttgc ttcttgtacc cggtgatttc cattaaatca ctgatcgtta aatacttccc
     1501 gaacaccttt cttgatggct cggggcgttg catgctgcga agcagctccg tgaattcact
     1561 cactgtgagt gaatataacg gacggtcaaa aaaattgttt gatgttttca tgaccacaaa
     1621 acaataggtg caaagtggtg gaaagtggtg gcgtaagaaa aatcattacg cacctatatg
     1681 aaagaatatc aaatagatgc aattttatga cacaaaaaaa gtggtggtaa aagtggtggc
     1741 cttattatga tttttactaa atgcaagatt aattccccaa caacaccccg ccggcaatat
     1801 cccagaacct ggtgaagaag tcaatgagtt tgcccaggac ggttt
//
LOCUS       DAOTGZ010000346         1810 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_3064
            NODE_76976_length_1810_cov_4.393732, whole genome shotgun sequence.
ACCESSION   DAOTGZ010000346 DAOTGZ010000000
VERSION     DAOTGZ010000346.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868088
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 1810)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1810)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:22:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,785
            CDSs (total)                      :: 2,751
            Genes (coding)                    :: 2,732
            CDSs (with protein)               :: 2,732
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 12 of 19
            Pseudo Genes (internal stop)      :: 5 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1810
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_76976_length_1810_cov_4.393732"
                     /isolate="AMR_MDS_3064"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            328..1233
                     /locus_tag="P5123_13875"
     CDS             328..1233
                     /locus_tag="P5123_13875"
                     /inference="COORDINATES: protein motif:HMM:TIGR01297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation diffusion facilitator family transporter"
                     /protein_id="HRX17395.1"
                     /translation="MQKEKDIRRVTIAGMLLNIILAAVKFVVGTAGNSQAVIADAFHS
                     LSDIVSDIALIVGIKFWGNPPDDDHPYGHQKIESIVTLFIGILLMLVAVKLVWNAVDS
                     FGNSRSSDTLIIAVAGPLLSIVFKELLYRVTVIIGKRHKSPAVIANAWHHRSDSISSV
                     PALIAVVVTAFFPKLNFVDQIGAVVVSLFILKVSFDIAKPAFEVLTDRTHNAELIASI
                     EKELVNNHEILSFHKIRARNAGGNYFVDLHIQVNGKLTVMEGHRISSELKYSILKSNE
                     DIVDVLIHLEPFARECLNDNVVQNI"
     gene            1365..>1810
                     /locus_tag="P5123_13880"
     CDS             1365..>1810
                     /locus_tag="P5123_13880"
                     /inference="COORDINATES: protein motif:HMM:NF016648.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FecR domain-containing protein"
                     /protein_id="HRX17396.1"
                     /translation="MKRIIIVVLLIVISVVAGFAWYLVKSEKMIYRAPALVHEKAIVT
                     FVVGDVTVKKNGAEEWQHVIVGQSVEPGDELQTGTSALADIRFHSSMAVRITENSHLV
                     MDANSIKKMELNLQSGSLYGKFHKLYDEHALVIKTVTSIAAVRGTD"
BASE COUNT          503 a          285 c          378 g          644 t
ORIGIN      
        1 ttattgtaaa gctgtcaatt ttatatctgt cagttatatg tcgtaaatgt aaaatttatc
       61 ctttgcataa aaacgcataa cgtttatctt tgagatgata aatttgcttg ccactggttg
      121 cataatgggt ttctacgaac taatcataat gttttttacc atgtctattt tctgatttga
      181 ctattaatca aatactattg attgtgctgt gatttcttta tatattgcaa attaacctgg
      241 ttaatatact tattttgttt aaagtgagat ttgactggtc gatattttat ggatgttttt
      301 tatttcattt acccaaaagg agaggtcgtg cagaaggaaa aagatataag gcgtgttaca
      361 atcgcgggta tgctactaaa tattatattg gccgctgtta aattcgttgt tggtacagca
      421 ggaaacagcc aagcggttat cgccgatgct tttcacagtt tatctgatat tgtgtcggat
      481 attgcgctta ttgtcggtat taaattttgg ggaaaccctc ctgatgatga ccatccctat
      541 ggacatcaga agattgaatc aatagttaca ctatttatcg gtattttgct tatgttggtt
      601 gcggtaaaac ttgtatggaa tgcagttgat tcttttggga actctcgatc ttctgatact
      661 ctaataattg ctgtagctgg tccgcttctt agtattgtct ttaaagaact tttgtatcgt
      721 gtaactgtca ttattggaaa aagacataaa tcgccggcag ttattgcaaa tgcctggcat
      781 catcgttcgg attccatcag ttctgttccg gcgttaattg ctgttgtcgt aactgctttc
      841 tttcctaagc tcaattttgt agatcagatt ggtgcagttg tcgtttccct gtttattcta
      901 aaagtatctt ttgatattgc aaaacctgct tttgaggtat tgaccgaccg gactcataac
      961 gcagagctta ttgcttctat tgaaaaagag ttggtaaata atcacgaaat tctttctttt
     1021 cataaaattc gtgctcgtaa tgcgggtggt aactattttg ttgaccttca tatacaggtc
     1081 aacggcaaat taactgtaat ggagggacat cgcataagta gcgagttgaa atactccata
     1141 ttaaagagta atgaggatat tgttgatgtt ctgatccatc tggaaccgtt tgcacgggag
     1201 tgtctgaatg ataatgtcgt gcaaaatatt tagtttatgt tatgaaaata ataactgttt
     1261 gacaaatggt tatttatagg tacaaatgag agcaatattt ctttccatac aattgttgct
     1321 atacaggata aatttcgcgg aattttccgc tgacgcgagg gattatgaaa cgcatcatta
     1381 ttgtagtatt gcttattgtt atatcggttg ttgccggttt tgcatggtat cttgtaaaat
     1441 ctgaaaaaat gatatatagg gctccggcac ttgttcatga aaaggccatt gtgacctttg
     1501 ttgttgggga tgttactgtg aagaaaaatg gtgcagagga gtggcaacac gttatagtgg
     1561 ggcaatctgt cgaaccgggt gatgagttac agactggaac atcagctttg gctgatatac
     1621 gttttcactc ctccatggct gtccgtataa cggaaaattc tcatcttgtt atggacgcaa
     1681 atagtatcaa aaaaatggag cttaatcttc aatcaggttc tctttatggt aaatttcata
     1741 aattatatga cgagcatgcg ctcgtgataa agactgtaac ctcgatagct gctgtacgtg
     1801 gaactgattt
//
LOCUS       DAOTGZ010000347         1805 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_3064
            NODE_77285_length_1805_cov_3.819429, whole genome shotgun sequence.
ACCESSION   DAOTGZ010000347 DAOTGZ010000000
VERSION     DAOTGZ010000347.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868088
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 1805)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1805)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:22:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,785
            CDSs (total)                      :: 2,751
            Genes (coding)                    :: 2,732
            CDSs (with protein)               :: 2,732
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 12 of 19
            Pseudo Genes (internal stop)      :: 5 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1805
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_77285_length_1805_cov_3.819429"
                     /isolate="AMR_MDS_3064"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1805)
                     /locus_tag="P5123_13885"
     CDS             complement(<1..>1805)
                     /locus_tag="P5123_13885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015695503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AIR synthase-related protein"
                     /protein_id="HRX17397.1"
                     /translation="KLSRDMVLSMDIEELRAIQNYYRDEKVLKKREKSGLPSIPTDCE
                     LEILAQTWSEHCKHKEFAAKITYTNRVTGETKEIDSLFKTYIKGSTEKIRENLKKNAN
                     DWLLKVFNDNAGVVELNKKDTFIWKVETHNSPSAIDPYGGAITGILGNNRDPLCTGIG
                     GGRLLFNTNVLCFGDPYYEGELIDTQLHPRRIFEGVVKGIEDGGNKSGIPTVNGSIIF
                     DERYAGKPLVYCGTAGIIPREYKGRNAWEKHIDKGDRIIMAGGRVGKDGIHGATFSST
                     ELNEFSPSSAVQIGSPITQKLLADFLERATERGLIKCSTDNGAGGLSSSIGELATISN
                     GAIVDLENVPLKYSGLKPWEVFLSESQERMSLVVEPRYIKEVFNLADEMEVELTDIGE
                     FSSEGDLVIRYDGKTIASLDLDFLHDGVPRKHMDAQWDGVDLKEPTLPEIDDYNMILK
                     KLLAALNICSKEWVIRQYDHEVKGKSVLKPLMGDGNSPQDAAVVRTSFDSFEGVAVSN
                     GIIPRYGDIDAYQMSAGSFDEAVRQIISAGGELPNLEKSNGIFWTVNDNFCVPDSLYD
                     PVNNPDGKVKLAKLVQMCEALYDMSTFFDIPMTSG"
BASE COUNT          516 a          431 c          312 g          546 t
ORIGIN      
        1 ttgccggatg tcattggaat atcgaaaaag gtactcatat cataaagtgc ctcacacatc
       61 tgaacaagct tagcaagttt aacctttcca tcaggattgt taaccggatc gtaaagacta
      121 tctggcacac aaaaattatc gttaactgtc cagaatattc cattgctctt ttcaaggtta
      181 ggaagctcac caccagcaga tattatctgt cgaacagcct catcaaaaga tccagctgac
      241 atctgataag catctatatc tccataacgg ggaataatac catttgaaac tgcaacccct
      301 tcaaaagaat caaaagatgt tcgcacaaca gctgcgtcct gaggagaatt tccgtctccc
      361 ataagtggtt taagcacgct cttgcctttt acctcatgat catactgacg aataacccac
      421 tcttttgaac atatattaag cgccgccagt aactttttta aaatcatgtt atagtcatct
      481 atttctggaa gcgttggttc ctttaaatcg acaccatccc attgagcatc catatgtttt
      541 cgaggcactc cgtcatgcaa gaagtcaaga tcaagcgaag caatagtttt accatcataa
      601 cggataacaa gatcaccttc agagctgaac tcgccaatat cagtcaattc gacttccatc
      661 tcatccgcca aattaaaaac ctcttttata tatcgcggct caacaacaag actcattctc
      721 tcttgcgatt cggacagaaa gacttcccat ggttttagcc cggaatattt aagtggaaca
      781 ttttcaagat caactattgc accgttagat atcgttgcaa gttcacctat agatgaagac
      841 aatccaccgg ctccgttgtc tgtactacat tttattagac cacgctctgt tgcccgctca
      901 agaaaatcag ctagtagctt ttgtgttatc ggagaaccaa tttgtacggc actcgacggt
      961 gaaaactcgt taagctctgt agacgaaaat gtagcaccat gaattccgtc tttacctacg
     1021 cgtccgcccg ccataattat gcggtcgccc ttgtcgatat gtttttccca ggcattgcga
     1081 cctttgtatt cacggggaat tattcctgct gttccacaat acacaagcgg tttaccggca
     1141 tagcgttcat cgaagattat tgaaccatta acagtcggta ttcccgactt attaccacca
     1201 tcctcgatac cctttacaac accttcgaaa attcgtcgcg gatgaagctg cgtatcaatc
     1261 agttctccct cgtaataggg atcgccaaaa cagagtacat ttgtgttaaa aagaagacgc
     1321 cctccaccga ttccagtaca gagtggatct ctgttgttac ctaaaattcc tgttattgct
     1381 ccaccatatg gatcaatagc cgaaggagaa ttgtgagttt cgactttcca gataaatgta
     1441 tcctttttgt taagttcaac aactccggca ttatcattga aaacctttag caaccagtcg
     1501 tttgcatttt ttttgagatt ttcccgaatc ttttctgtag agcccttgat atatgtcttg
     1561 aaaagagaat caatctcctt tgtttcgcct gtgactctat ttgtatatgt aatcttcgcg
     1621 gcaaattcct tatgcttgca atgttctgac catgtttgag caagaatttc aagctcacaa
     1681 tcggtaggta tggaaggaag tccggacttt tcccgttttt ttaaaacctt ttcatccctg
     1741 taataattct gtatagccct aagttcttca atatccatag aaagaaccat gtctcttgac
     1801 aactt
//
LOCUS       DAOTGZ010000348         1769 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_3064
            NODE_79414_length_1769_cov_6.471995, whole genome shotgun sequence.
ACCESSION   DAOTGZ010000348 DAOTGZ010000000
VERSION     DAOTGZ010000348.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868088
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 1769)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1769)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:22:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,785
            CDSs (total)                      :: 2,751
            Genes (coding)                    :: 2,732
            CDSs (with protein)               :: 2,732
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 12 of 19
            Pseudo Genes (internal stop)      :: 5 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1769
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_79414_length_1769_cov_6.471995"
                     /isolate="AMR_MDS_3064"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..546)
                     /locus_tag="P5123_13890"
     CDS             complement(<1..546)
                     /locus_tag="P5123_13890"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRX17398.1"
                     /translation="MEAIKWNSDSITVLNAQLLPDKSYYHNYKNFREIGAAVEAKIIP
                     GTVSRAIAVVMSASLTAEQSVNLKADQFKEHLLFVVDYFEKITLANPLTGGILSKLRN
                     EVAGSSNNTETVKKFKEMSVSFFSDLHDRCAAVAENVQHTMVSGESILLFSAAGSSLA
                     APAGGFITSALIAAKKNDKQIS"
     gene            complement(524..1471)
                     /locus_tag="P5123_13895"
     CDS             complement(524..1471)
                     /locus_tag="P5123_13895"
                     /inference="COORDINATES: protein motif:HMM:NF024970.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HRX17399.1"
                     /translation="MINEHGGSTKTYTVVVVDDEEIVRYTMRKKLARFGYKVISLDKG
                     EDALYLIKNSAERIDFLVTDIKLRKMDGIELLRHINSLEAPVPVLLTGNGNVEDAVKA
                     LRYGACDFIKKPVDVNQIASIIRHVLKVREEERKYINLGRFSVYEKREFLIPNDPELA
                     NLLAFELTKNLPGIGLCSKAETENILLALREALSNAIYHGNLEVSSDLRESDGGVREF
                     NEEVERRCELSPYKERKVRVQYELTNEFVEYVIIDEGPGFDFGSLPDPKDPENFFKKS
                     GRGLLIIKIHMDEVEWRSPGNFLRLKKYRVETDGSNKVE"
     gene            complement(1461..>1769)
                     /locus_tag="P5123_13900"
     CDS             complement(1461..>1769)
                     /locus_tag="P5123_13900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013637630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     3,4-dihydroxy-2-butanone-4-phosphate synthase/GTP
                     cyclohydrolase II"
                     /protein_id="HRX17400.1"
                     /translation="YHLQEHGFDTVEANIKLGFPSDLRDYGIGAQIIADLGVSTIRLM
                     SNNPKKIIGLEGYGLKVVERVPIEIEPIDENIDYMKTKKDKMEHILNSEKLSGGGDDK
                     "
BASE COUNT          518 a          413 c          288 g          550 t
ORIGIN      
        1 actgatttgt ttatcattct tttttgctgc aataagagcc gaggtaataa atccaccagc
       61 aggagcagca agtgatgaac cagctgcaga aaacaaaaga atggactcac cagataccat
      121 tgtatgttga acattttcgg ctacagccgc gcatctatca tgcagatcag aaaaaaacga
      181 gacggacatc tctttgaatt tttttacggt ctccgtattg ttgctacttc cggctacttc
      241 atttcgtaat ttggagagaa ttccaccagt taagggattt gcaagagtta tcttttcaaa
      301 ataatcgaca acaaataaaa gatgctcttt aaactgatca gcttttaaat taaccgactg
      361 ttctgccgtt aaagaagccg acataacaac agctatagca cgggaaaccg tgccgggtat
      421 aatcttggcc tcaacagccg ctcctatttc cctgaaattc ttataattat gataataact
      481 cttatccggc aaaagttgtg cgtttaacac agttatcgaa tcactattcc actttattgc
      541 ttccatctgt ttccactcta tattttttca gtctaagaaa atttcccgga gaacgccatt
      601 ctacttcatc catatggatc ttgataatta gcaaaccccg gccacttttc ttgaagaaat
      661 tctctggatc cttcggatca ggcaatgagc caaaatcaaa cccgggtccc tcatcaatta
      721 ttacatattc aacaaactca tttgtcaatt catattgaac acgaactttt ctttctttat
      781 aaggtgagag ctcacatctt ctctcaacct cttcgttaaa ttccctgaca ccaccatcac
      841 tttcgcgcaa atctgatgag acctccagat ttccatgata aatagcattg gataaagcct
      901 ctcgaagagc taaaagaatg ttttcggtct cggccttgct gcatagtcct atacctggaa
      961 gatttttagt gagctcaaaa gctaaaaggt tggccaactc gggatcatta ggtattaaaa
     1021 actcccgttt ttcgtaaaca gaaaaacgcc caagatttat atatttacgc tcttcctcac
     1081 gaacttttaa aacatgtcta ataatcgacg ctatttggtt aacatcgaca ggttttttta
     1141 taaaatcgca ggccccgtaa cgcaaggcct taacagcatc ttcaacattg ccattgccag
     1201 tcagcaaaac aggtactgga gcctcaagag aattaatatg tctgagtagc tctattccgt
     1261 ccattttgcg taattttatg tcagtaacca gaaaatctat acgttcggct gaattcttta
     1321 tcaaataaag cgcatcttcg cccttatcaa gagatatgac cttgtaacca aaccgtgcga
     1381 gtttctttcg catggtatat cgtactatct cttcatcatc aacaaccaca acggtatagg
     1441 ttttggtact gccaccatgc tcatttatca tcgccgccgc cagataattt ttcagagttt
     1501 agtatatgtt ccatcttgtc ttttttggtc ttcatataat cgatattctc gtcaatcggt
     1561 tcaatttcaa taggcacacg ctcgacaacc tttaatccgt aaccttccaa tccaatgatt
     1621 tttttaggat tgttgctcat tagccttatt gttgacacgc caagatcagc tattatctga
     1681 gctcctattc cgtaatcacg caaatccgaa gggaacccca gctttatatt cgcctctaca
     1741 gtatcaaatc catgctcttg cagatgata
//
LOCUS       DAOTGZ010000349         1708 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_3064
            NODE_83532_length_1708_cov_3.434362, whole genome shotgun sequence.
ACCESSION   DAOTGZ010000349 DAOTGZ010000000
VERSION     DAOTGZ010000349.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868088
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 1708)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1708)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:22:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,785
            CDSs (total)                      :: 2,751
            Genes (coding)                    :: 2,732
            CDSs (with protein)               :: 2,732
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 12 of 19
            Pseudo Genes (internal stop)      :: 5 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1708
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_83532_length_1708_cov_3.434362"
                     /isolate="AMR_MDS_3064"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            185..682
                     /locus_tag="P5123_13905"
     CDS             185..682
                     /locus_tag="P5123_13905"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRX17401.1"
                     /translation="MKSKKLFYIGCIITVLNLLAVINGMYFFFGMAGFSFVEWFFFNI
                     CAPSTIIFITGFALRKIWLMSAAVPLMIFYGVGGLFIFGWSGTALFSQAGHLLMSAAI
                     IFTGVSIWSNREHLRAGLAGLVAGVLLFMLVFPFQQSFVTNRMDLVKKLQDPAFEEFI
                     KNQGD"
     gene            686..1519
                     /locus_tag="P5123_13910"
     CDS             686..1519
                     /locus_tag="P5123_13910"
                     /inference="COORDINATES: protein motif:HMM:NF018184.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HRX17402.1"
                     /translation="MYSIGEFSRITRISTKTLRHYHQWGILLPDYVDDENGYRYYRPQ
                     SVERAFLISELRRLEFSIADIREILASSSKDCDLLEYLQSRSNEVRNLAAKYHRLEEE
                     LETAINTIRGNEMKESFSLVIKEKDIEDMLFAGYRFTGRYDQVGKAFSTIWKNCGRFV
                     NGKAMTLYYDSEYKERDADIEGGCQVREGCALKKFSDNNINVHKIEGGRAVTLIHSGR
                     YEDIGASYKLIFDYIKDKGYDVRLPTREIYLKGPGIFFKGNPDKYKTEIQIFLKGNFE
                     K"
BASE COUNT          491 a          266 c          381 g          570 t
ORIGIN      
        1 tatattgcaa gtcaatatat atttaagtaa tttcgtgata tttataatac gagttataat
       61 aagtttagaa agtaaggttt ttactgatag aaaaaacctt tgactttacc cccgcgggaa
      121 ccctgatctt tgtttcagca tgtgagtgtt aaaacattgg gtgcaaattc ccttggaggt
      181 tgtaatgaag tcaaaaaaac tgttttatat cggttgtatt ataacagttc ttaatctgtt
      241 agctgttatt aatgggatgt attttttctt tggaatggcg ggattctcat ttgtggagtg
      301 gttctttttt aacatctgtg caccgtctac cataatcttt atcacagggt ttgctctacg
      361 taaaatctgg ctaatgtcgg ctgcagtgcc attgatgatt ttttacggtg tcggaggtct
      421 ctttattttt ggttggagcg gtacggctct tttttctcag gcgggtcatc ttcttatgag
      481 tgctgctatt atttttactg gtgtatccat ctggtctaac cgcgagcatc tgcgagctgg
      541 ccttgccggt cttgtcgcgg gagttctgct ttttatgctt gttttccctt ttcagcaatc
      601 atttgttact aacagaatgg atcttgttaa aaaattacaa gatccggcat ttgaggagtt
      661 cattaaaaat cagggagatt gattgatgta cagtatagga gaattctcgc gaataacaag
      721 aatatcgaca aaaacgctgc gtcattatca tcagtggggt attttgctac ccgattatgt
      781 agatgacgaa aatggttacc gatattatcg accacaatcg gtagaacgtg cctttttgat
      841 aagtgaactt aggcgtcttg aattctccat cgcagatatt cgggagattt tagcatcttc
      901 tagcaaggat tgcgatcttc ttgagtatct tcaaagtcgc agcaatgagg tcagaaatct
      961 tgctgcgaaa tatcaccgtc ttgaggagga gcttgagacg gcaattaata ctattagggg
     1021 taatgagatg aaagaatcat tttcgctggt tattaaagaa aaagatatag aagatatgct
     1081 ttttgcgggt tatcgtttca cgggaagata cgaccaggtt gggaaggcgt tttctacaat
     1141 ttggaaaaac tgtggtcgct ttgttaacgg taaagcaatg accctctatt atgattccga
     1201 gtacaaagag agggatgctg atattgaagg tggctgtcag gttagagagg gttgcgcttt
     1261 gaaaaaattt tctgacaata acattaatgt acacaagata gagggtggaa gagccgttac
     1321 attgattcat tctggtcgtt atgaagatat cggtgcatcc tacaaactta tttttgacta
     1381 tatcaaagat aagggatatg atgtccgtct tccaaccagg gagatttatt taaaggggcc
     1441 ggggatattt tttaaaggta atccagataa gtacaaaaca gaaattcaga tttttttgaa
     1501 agggaacttc gaaaaataac cttttttgga acggctatgc tgtcaatagc tagattaaag
     1561 acaatttttc tcgttaacac tcgaaaaaac gctcataaat atcgctttga ggccagcgat
     1621 atttatggct ttattaaagg gatttttcgc aagttccatg ttgaaaagtt aattaataga
     1681 gattgccaat atagggtagt ttataata
//
LOCUS       DAOTGZ010000350         1586 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_3064
            NODE_92782_length_1586_cov_4.221424, whole genome shotgun sequence.
ACCESSION   DAOTGZ010000350 DAOTGZ010000000
VERSION     DAOTGZ010000350.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868088
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 1586)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1586)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:22:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,785
            CDSs (total)                      :: 2,751
            Genes (coding)                    :: 2,732
            CDSs (with protein)               :: 2,732
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 12 of 19
            Pseudo Genes (internal stop)      :: 5 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1586
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_92782_length_1586_cov_4.221424"
                     /isolate="AMR_MDS_3064"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>1586
                     /locus_tag="P5123_13915"
     CDS             <1..>1586
                     /locus_tag="P5123_13915"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004427470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="chemotaxis protein CheA"
                     /protein_id="HRX17403.1"
                     /translation="FVGLMDLSELAHKMENLLQGVRDKTMKITPEMVDLLFKCFDHIN
                     LVIGKVSQGEEPIVDLSGIIEEIGQMQGKSSLAAEEKKEEAATDTGSSMSENIPKTTF
                     SGQEKRHLKRGVDVGLSCYELTIFIEPDAPMKWVKAQLILNNIKNIAQVVKTIPDEEN
                     LTDEAIRDVLKLIILTDEDLREIRSACDVDLVNRIDILKISMEKKDNKFFIRFGDKQI
                     LSETQDEVVSTENDEKESANSESAVVSYADEEDDADISESETMSVDFSGYNDRRESGG
                     ISRNAPVLRTVKVSVDKLDDLLNNVGELVIANSGFFRLYEDLKKIQLGKNVTNEFKNR
                     IEQMARIAKDLQSGIMKTRMVPIGQVFSRFHRLIRDVSKECGKNVQLVTRGEDTELDK
                     KVIDVIGEPLMHMIRNSIDHGIETPDERKSLKKSEVATVTLSAHQGGNQIFVEVIDDG
                     RGLDTEVIRAKAVAKGLVTADQAGNLDTNEIYNFIFHPGFSTAKKVTDVSGRGVGMNV
                     VREVVQEMGGNITIESEQGM"
BASE COUNT          481 a          261 c          396 g          448 t
ORIGIN      
        1 gttttgttgg tcttatggat ttaagtgagc ttgctcacaa gatggaaaat cttcttcagg
       61 gtgttcgcga taagaccatg aagatcactc cggaaatggt tgatctttta ttcaagtgtt
      121 ttgaccatat taatcttgtt ataggtaagg tttctcaggg tgaagaaccg atagtggatc
      181 tctcgggcat tatcgaagaa attgggcaaa tgcagggtaa aagttctctt gctgccgaag
      241 aaaaaaaaga ggaagccgca acagatactg gaagctccat gtctgaaaac attccgaaga
      301 ctactttttc cggacaggag aagcggcatc taaagcgtgg tgtagatgta gggcttagtt
      361 gctacgagtt gacaattttt attgaacctg atgctcccat gaaatgggtc aaggcacagc
      421 tgattttaaa taatataaaa aatattgccc aggttgttaa gaccatacca gacgaagaaa
      481 accttactga tgaggcaatt cgtgatgttc taaagcttat tattttgacc gacgaagact
      541 tgcgggagat aagaagtgcc tgcgatgtgg atctggttaa ccgtatagat attcttaaga
      601 taagcatgga aaaaaaagac aacaagttct ttatacggtt tggtgataaa caaattttat
      661 cagagacaca ggatgaagtt gtatctactg agaatgacga gaaggaatct gccaattctg
      721 aatcggctgt tgtgtcatat gcagatgaag aggatgacgc agacatttct gaatctgaaa
      781 ccatgtccgt tgatttctct gggtataacg atagaagaga gtccggtggc ataagtcgaa
      841 atgcacctgt tttgagaact gtcaaggtct ctgttgacaa acttgatgat ttgttaaaca
      901 atgtaggtga acttgtaatt gcgaactccg gttttttcag attatacgag gatctaaaaa
      961 agattcagct tggcaaaaac gttactaacg agttcaaaaa ccgtattgaa cagatggctc
     1021 gaattgctaa agatcttcaa agcggtatta tgaaaacccg gatggttcca ataggtcagg
     1081 ttttttctcg gtttcatcgt cttatccgtg atgtctccaa agagtgtgga aaaaatgtac
     1141 agcttgttac tcgtggagag gatacagagc tggataaaaa agttatagat gtaattggcg
     1201 agccactgat gcatatgata cgtaatagta ttgatcatgg tatcgaaact cctgatgaac
     1261 gtaaatccct taaaaagtca gaggttgcta cggtgaccct gagtgctcat cagggcggta
     1321 atcagatttt tgtcgaggtt attgatgacg ggcgtggttt ggatacagag gttatccggg
     1381 ctaaggccgt agcaaaaggt ctggtgacag ccgatcaggc cggtaatctt gatactaacg
     1441 agatatacaa ttttattttt catcccggtt tctcgacagc taagaaagtt actgatgtct
     1501 cgggacgcgg tgtgggaatg aatgttgtac gcgaagttgt ccaggagatg ggtggcaata
     1561 ttaccatcga gagtgaacag ggcatg
//
LOCUS       DAOTGZ010000351         1543 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_3064
            NODE_96493_length_1543_cov_5.359543, whole genome shotgun sequence.
ACCESSION   DAOTGZ010000351 DAOTGZ010000000
VERSION     DAOTGZ010000351.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868088
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 1543)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1543)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:22:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,785
            CDSs (total)                      :: 2,751
            Genes (coding)                    :: 2,732
            CDSs (with protein)               :: 2,732
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 12 of 19
            Pseudo Genes (internal stop)      :: 5 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1543
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_96493_length_1543_cov_5.359543"
                     /isolate="AMR_MDS_3064"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            128..940
                     /locus_tag="P5123_13920"
     CDS             128..940
                     /locus_tag="P5123_13920"
                     /inference="COORDINATES: protein motif:HMM:TIGR01484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IIB family hydrolase"
                     /protein_id="HRX17404.1"
                     /translation="MIRICYSDLDGTFLDHLSYSYKLSLSGYSLLLDRNIPLVFVSSK
                     TFVEIEQLEFMLNSRSPFIFENGAGVAFADDSGKYSYKLLSNFNSSKLDLVKESLKQA
                     AKETVFFDEISPEQLAEISGLSLDRARLSLSRIATLPFYINGSSISFEEIRKIVLPFD
                     LEITKGGRFYHLVPVGCSKGIALKYIDEYYKKKCNDSVVTMGVGDSLNDIPMLEMVDY
                     PFLVLKHDGSCLTDTKYKLIDKPGPAGFSEAVQQFCIDTDTININCESSEEI"
     gene            937..1395
                     /locus_tag="P5123_13925"
     CDS             937..1395
                     /locus_tag="P5123_13925"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRX17405.1"
                     /translation="MSNALTGLFIDVFKGIFDFFMRIFSALLNWFMELIIWERLIIIN
                     FVTAFFAVLLPVAKFYILDFWYGISNPLAINLIGLVFVMFATVFLPFFLTMLVRVTLN
                     VLYIVHLLIIVLSGSLTHAPEYNYSIGMLLNFFAPLAFIILSLLSYRDRK"
BASE COUNT          436 a          234 c          304 g          569 t
ORIGIN      
        1 taaagaatga gtcagactct aaggttgtat accagtacgt aaaagatctg ataaaactga
       61 gagggtatga ggtcgatata aaaattatat ctagtgacaa aaatattatt attgctgaaa
      121 cgaaattatg atacgtatct gctacagtga tcttgacgga acctttcttg accatttaag
      181 ctattcgtat aaactttcct tgtctgggta ctcattgttg ttagatagaa atattccctt
      241 ggtttttgtt tcgagtaaaa cttttgttga aatcgagcaa cttgaattta tgttgaattc
      301 cagatcacca tttatttttg aaaatggtgc gggtgtagct tttgcggatg attcaggcaa
      361 gtatagttat aagcttttat caaattttaa tagttctaag cttgacctag ttaaagagag
      421 tttgaagcaa gctgcaaaag aaacagtttt ctttgatgag attagcccag agcaacttgc
      481 tgaaattagc ggattatccc ttgatagagc acgcttgtcg ttatcgcgta ttgcaacact
      541 gccattttat attaacggtt cgtctatctc tttcgaagaa atcagaaaga ttgttttgcc
      601 atttgatttg gaaattacaa aaggtggtcg tttttatcac ttagttcctg tcggttgctc
      661 caagggaatt gctttgaagt atattgatga atactataaa aaaaagtgta acgattccgt
      721 tgttacgatg ggagtaggtg attcgttaaa cgatattcct atgcttgaaa tggtggacta
      781 tccgtttttg gtattaaaac atgacggatc ctgtcttacc gatacaaaat acaagctgat
      841 tgacaaacct ggtccggcag gtttttctga agcagtacaa cagttttgta tagatacaga
      901 taccattaat ataaattgcg aatcttcgga ggagatatga gtaacgcttt gacaggccta
      961 tttattgatg tatttaaagg aatatttgat ttttttatgc gtattttttc ggctctatta
     1021 aattggttta tggagctaat tatatgggaa agactaataa taataaattt tgttacggcg
     1081 ttttttgctg ttttgcttcc agtagcaaag ttctatatac tggacttctg gtatggcata
     1141 agcaatccgc ttgctattaa tttgattggg cttgtctttg taatgttcgc cactgttttt
     1201 ttaccgtttt ttctcaccat gttagtgcgt gtaacattga atgttcttta tatcgtacac
     1261 ctgttaatta ttgttttgag cggatcactt acacacgccc ctgaatataa ttattccata
     1321 ggtatgcttt taaatttctt tgctccttta gcttttatta ttttgtcatt actttcatac
     1381 agagatcgta aatagagatg tttatcgact ggattggtta tttcgcctca gttttaattg
     1441 ctttgtcact tatgatgagt tcacacataa agttgcgctg gctaaatatg gcgggtgcaa
     1501 ttattatggt cgtatacggt attttgatag aatcgctgcc aat
//
